LOCUS       DAOSIB010000001        67232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_491_length_67232_cov_12.673177, whole genome shotgun sequence.
ACCESSION   DAOSIB010000001 DAOSIB010000000
VERSION     DAOSIB010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 67232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 67232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67232
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_491_length_67232_cov_12.673177"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..638
                     /locus_tag="PLD44_00005"
     CDS             213..638
                     /locus_tag="PLD44_00005"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HOF97754.1"
                     /translation="MITKKRIANFKWLSMISLLMFISIQHSVQAQTSSIPNEIVSALS
                     NGDANRLSAYLNDNVELFIENKNDIYSRQQAVSILNDFFHRFKVSDFKVIHQGNRDNS
                     SFIIGILSTATENFRVYILTRKSDNKTIILQLRIETSNE"
     gene            631..1506
                     /gene="nadC"
                     /locus_tag="PLD44_00010"
     CDS             631..1506
                     /gene="nadC"
                     /locus_tag="PLD44_00010"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOF97755.1"
                     /translation="MNEDYKQLIPLWFAEDIGDGDHTTLACIPETALGKSQLIIKENG
                     VIAGVEVAREIFKTFDPKLKMNVFITDGTEVKIGDIAFNVEGKIQSLLQTERLMLNVM
                     QRMSGIATRTRQYVKALEGTKTRVLDTRKTTPGLRFLEKEAVRIGGGVNHRFGLYDMI
                     LLKDNHIDFAGGIKEAILRTKEYLKNKGKNLKIEIEVRNFDEIEQVMAIGGVDRIMLD
                     NFNIENTKKAVEIIGGRYEIESSGGITFDTLRQYAECGVDFISVGALTHSVKSLDMSF
                     KADIPNLIEGIHKIF"
     gene            1543..2091
                     /gene="rfbC"
                     /locus_tag="PLD44_00015"
     CDS             1543..2091
                     /gene="rfbC"
                     /locus_tag="PLD44_00015"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOF97756.1"
                     /translation="MEIIETPIPDLLIIRPKVFEDARGYFYETYNEKSFMEAGLDIHF
                     CQDNQSKSVYGVIRGLHYQLSPCSQCKLVSAIQGTVWDVAVDLRASSPTFGKWFGIEL
                     SAENHLQFLIPKGFAHGFSVLTETAIFAYKCDAFYNPSLERGILYNDPQLAIDWKIPE
                     DKAIVSAKDAKHPIFSKAEKNF"
     gene            2134..3003
                     /gene="rfbD"
                     /locus_tag="PLD44_00020"
     CDS             2134..3003
                     /gene="rfbD"
                     /locus_tag="PLD44_00020"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOF97757.1"
                     /translation="MQTILITGSKGQLGNELQKAAKRFPNFRYIFTDVDELDICNKSA
                     LDNFVKENRIEIIVNCAAYTAVDKAEEEVEKCFAINAQAVKNIGEIAETYQIKVVHIS
                     TDYVFDGKSYKPYTEEMEVSPDTVYGKSKLQGEKFLQSVCNQYIVIRTSWLYSSYGNN
                     FVKTMLRLGKERNSVNVIFDQIGTPTYAADLAEIILKILSQNNFISGIYHFSNEGVCS
                     WYDFATAIFKIAHVNCLVHPIETKDYPAPAPRPYYSVLNKSKIKNTFGLSIAHWEESL
                     EKCIEILKSEYSL"
     gene            3178..3900
                     /locus_tag="PLD44_00025"
     CDS             3178..3900
                     /locus_tag="PLD44_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOF97758.1"
                     /translation="MGRAFEYRKATKMKRWGNMARVFTKLGKQITIAAREGGPDPDMN
                     PRLRVLFQQAKKENMPKENVERAIKKAVSKEEADNYKEVIYEGYAPHGIAVIIETATD
                     NPTRTVASIRSYFNRYGGSLGTSGSVQFLFDHKCVFRIAPKSNLSIDDLELELIDYGV
                     DEVIQDDEENNIVLYGDFQHYSSIQKYLEENNFEIFSAEFEWIPKETKELNDEQKAQV
                     LKLIEKIEDDEDVQNVFHNMAE"
     gene            4010..5158
                     /locus_tag="PLD44_00030"
     CDS             4010..5158
                     /locus_tag="PLD44_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOF97759.1"
                     /translation="MDISVLFPALASLTWQHILMILIGLTLIYLAIFKEYEPTLLLPI
                     GFGTILVNIPLSSAINQIDGKTGEIIEGILSVFYEGGIATEMFPLLIFIAIGAMIDFT
                     PLFNNPHLLLFGAAAQFGIFMTMFIATLFGFDLKEAASIGIIGAADGPTSIFVASRFA
                     PHLLGPISVAAYSYMALVPIIQPPVIKLLTSKKERLIRMQYSSKKVSRKALIAFPIIV
                     TIISGLIAPASLSLIGFLMFGNLIRECGVLNKLSQSAQNELASLVTILLGITIASTMT
                     AERFVTFGTLTIIALGLLAFVFDTFGGVIFAKLLNLILPAKKKMNPMIGACGISAFPM
                     SARVIHTMGLKEDPHNFLLMPAVSANVGGQIGSVVAGGLILTLVNLFA"
     gene            5171..5308
                     /locus_tag="PLD44_00035"
     CDS             5171..5308
                     /locus_tag="PLD44_00035"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG-related small transporter subunit"
                     /protein_id="HOF97760.1"
                     /translation="MINNVEAALEIGAIGITGIFIFMLIFYAAIKLILKLFPEKENKE
                     E"
     gene            complement(5429..6247)
                     /gene="panB"
                     /locus_tag="PLD44_00040"
     CDS             complement(5429..6247)
                     /gene="panB"
                     /locus_tag="PLD44_00040"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOF97761.1"
                     /translation="MSVHNEDNRKVTTRRLFEMKQRGEKISMLTAYDYTMANIVDEAG
                     IDVILVGDSASNVICGNTTTLPITLDQMIFLAKSAIRGIKRALAVVDMPFGSYQGNSH
                     EAIASAIRIMKETHADGIKMEGGEEIIESVKRIIGAGIPIMGHLGLMPQSINKYGSYN
                     VRAQEEEEAEKLIRDAHLLEDAGCFALVLEKIPAKLGTQIASELSIPVIGIGAGGGVD
                     GQVLVIHDMLGLTQGFSPRFLRRYANLREQMNQAIQHYIADVKSSDFPNEKEQY"
     gene            6463..6927
                     /locus_tag="PLD44_00045"
     CDS             6463..6927
                     /locus_tag="PLD44_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOF97762.1"
                     /translation="MSKITIHSYQELEEYVGKELGVSDYLTITQEQINKFADATLDYQ
                     WIHVNAEKAKQESPYKNTIAHGYLNLSILPYLWGQIMEIRNSKMTLNYGIENARFQQA
                     VVVNDQVRAKAKLKSLVNLKGIAKAEIYTVLEIKNSNKPALEGTFIFLYHFE"
     gene            complement(6936..8612)
                     /locus_tag="PLD44_00050"
     CDS             complement(6936..8612)
                     /locus_tag="PLD44_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HOF97763.1"
                     /translation="MEWFLSLLLNDGIAHTILLFAFVISLGILLGKIKIFGISLGVTF
                     VLFVGLFVGHLGLSVNPEVLEFIKNFGLILFVYSIGLQVGPSFFSSFKKGGMLLNLLS
                     TMIVLLGVGIVIALYYLLHGKVSMPMLVGILSGSVTNTPGLGAAQEALRHVFGSSPGA
                     EIPPIALGYAVAYPIGVLGIIFSMVLLRIIFKVDFHKETEQIKKASEVDEEQVEKISV
                     QVKNEKVFNHTVTDVKRAVGRPFIVSRLMRNGEFFIPQGDSMLYKDDILLIVASSRDI
                     ERITSYIGEKVEMDWKISEEKLVSRRIVITHGKINGKTIGSLKLRTIYGVNITRVNRS
                     GVDLLGTPDLVLQVGDRVMVVGELDAIEKVEKLLGNTLQKLNEPPIITIFVGILLGVL
                     VGSIPFFVPGIPVSVKLGLAGGPLIVAILLGRFGYKLKLITYTTTSANYMLREIGISL
                     FLASVGIGAGEKFVETVISANGLLWMGCAAAIALLPLLIVGIIGRKFLKINYFTLIGL
                     LAGSTTDPPALAYSNSIAGSDIPAVAYSTVYPLTMFLRVVSAQILILAFL"
     gene            8834..10030
                     /gene="hisB"
                     /locus_tag="PLD44_00055"
     CDS             8834..10030
                     /gene="hisB"
                     /locus_tag="PLD44_00055"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HOF97764.1"
                     /translation="MVHFRNKVHYKHINKINMEENTGKTVKKRVLFIDRDGTIIVEPS
                     ETFQVDSLEQLEFLPGVIRNLYFIRQRTDFEWAMVSNQDGLGTSAYPEENFEKVQSKM
                     LKILENEGIIFDKIFIDKSLPEEKLPTRKPGTAMLKEYFSANYDLENSYVVGDRITDV
                     ELAKNLGCKAILLNEDPEILQESKLKKVCVLQTTSWDEIAAFIFAGERTATIQRTTKE
                     TDIFIHLNLDGSGKGNISTGLRFFDHMLEQIARHSGCDLTIQVKGDLEVDEHHTIEDT
                     AIALGEAFAKALGDKRGIERYGFCLPMDDSLCLVAIDFGGRAWLTFDAIFKREYIGDF
                     PTEMIFHFFKSLSDAAKMNLYVKALGENEHHKIEGIFKALAKAIKMAVKRDIYQFELP
                     TTKGII"
     gene            10098..11675
                     /locus_tag="PLD44_00060"
     CDS             10098..11675
                     /locus_tag="PLD44_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOF97765.1"
                     /translation="MIFLLIVQFPLHAQIFPDQPRLVIGIVVDGLQEKHIDLLWQRFD
                     ANGFKKIIEKGTAFENMEYNIVSAGNASDIATLMTGTTPYYHGVTGNNFYSRKQGNVQ
                     SVIFDENEPGIGTKQTVSAHQLLASTITDELMLTYPKQSKTYAIAINAEDAIMLGGHT
                     ANSAMWLDDINMKWVTTSYYTDGLAASADEMNVNGQIEQYASSVWEPMYSPNTYLFPS
                     ANENKKNGFQYFPNSKRKGSNLPILKNTPNANALVADLALKIFEKENLGQDTYPDMLL
                     LQFNVKTPYEKTFALKTAEKEDMYLRLDKDLQYLFQKIDSKIGLSNTLIFILGTQTSA
                     HTPLELGENKIPAGYFNGRRSMALLNTYLMVLYGQERWVEGYYGKNIFLNRQKIEEKK
                     INLQEIQQVVADFMHEFEGIQSAYTSYQIVNLTDPSNREMSKLRNSFQKSSKGDVIIT
                     LLPGWLELDDNNNPIGESNAVISSTPVYFYGWNIEPKKIAAIHSIIDIAPTICKILKL
                     PFPNATTGKPLEEIINK"
     gene            11694..12869
                     /locus_tag="PLD44_00065"
     CDS             11694..12869
                     /locus_tag="PLD44_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOF97766.1"
                     /translation="MKKYDFDEIIDRTNTNAVKIEACRSMFGTSNVIPLWVADMDFRT
                     PDFILDSIRKRCEHPILGYSVPPKDYFPSIMNWLKENHGWEVKKEWLGFMPGVVPGLS
                     FAVQQFSAENDEIVIQPPVYFPFMHVVEKNNRKLIFNPLKEVDGKFQMNFDDLEKKIT
                     SKTKMLILCNPHNPGGRSWGKETLQQLAEICAKHHLIVVSDEIHADMTLPGNVHVPFA
                     SVSERAANISLTYMSPSKTFNIPALQTSYFIIPNEELRNGLKNFLDRSELDNGNIFAY
                     LSTIAAYTEGKWWKMQMLDYVQGNIDFVIQFLQNEIPQIKPMKPEASFLIWLNCSDLG
                     MNEEELFQFFVHKAGLGLNQGITFGPGGEQHLRLNVACPRSTLEKAMNQLKNAVRNL"
     gene            12880..13557
                     /locus_tag="PLD44_00070"
     CDS             12880..13557
                     /locus_tag="PLD44_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOF97767.1"
                     /translation="MWGTLVNALAVIVGGSAGLLLNKKMPQRIQTIYFQVIGLFTIGI
                     GITMVVGMKHILLIVMSLVIGVLIGEWMHLESRLEKWSESLKKRFQIGSEQFSEGFVT
                     AFLLFCIGSMTILGSIQEGLTGSPDLLFTKSLMDGFSAIILASAFGVGVIFSSIPLLI
                     FQGGLTLLAMAASSFFTPDLINGISNIGGILLIGLGINILEIKKLAITNMLPSLVVVI
                     LLIWGFA"
     gene            complement(13645..15234)
                     /locus_tag="PLD44_00075"
     CDS             complement(13645..15234)
                     /locus_tag="PLD44_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOF97768.1"
                     /translation="MKTKITVLLTLMLFCMQAIFSQNQDTLSSHLILFYELNGNGKNS
                     VSDNNHLKLKSQPGNYYYKWTTGKDGKDSTAVVFDGNAQGLKTHLDISPQNLPEITYT
                     AWVFGRPDGFLFGSTLPVNNEKKFSSRSLQISKGAVICGFNKLDSLKNPYFASLRSAT
                     LKKDKWNFIAMTANANDSTLRLYVNDEYYESKPVVFTKKSNFLIIGNTQNSPYNRAFK
                     GQVDEIRVYNKALSSDELSSISGISFADAQKRMERNETIQKVLLAGLIIFLFVSVIYM
                     IVVLITEKRYKPIKTANNTSFSADSKNARIDSEKEDANRLASECVEEAFDTWEPVSEE
                     NGQIFRSPTKRKHFKTTYRALNMAKSYQPTDNEVIDRMNDLGGLLNKLSKRKFYGNWF
                     LAVLSLIFPVVYFLLAKNHLDTKGTIGAVVMFLPTIAYILSNFAPNYVVANRKHNSGG
                     AFGKIIAFIMGAAASAVAVDYYTEKTWSDGSKTTEYDTGSNALQLVIWAVLIIAAILL
                     SMVLVIIAATIAFFRNYVFYL"
     gene            complement(15420..18581)
                     /locus_tag="PLD44_00080"
     CDS             complement(15420..18581)
                     /locus_tag="PLD44_00080"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOF97769.1"
                     /translation="MLKQYLNSLQATFNQGDAREESYYGHLKDLLLNYTDERQTNKVD
                     ITVLPKPTEAGNPDFRIWDGKNHITGYIEAKDPSVTNLDRIEVSGQLKRYRETFPNVI
                     LTNFYEFRLYRNGELIKQVLVGRPELARKLKTAPPVENETVFFDLLDTFFSFSLPHVR
                     NAQSLATELAKRTRFLRDEVVSIELDEEEKRGKKAILGFYEAFRKYLIGTLTKEAFAD
                     LYSQTLTYGLFAARTRSDNSFNRELAFKYIPNTIGILRDVFRFISLEDPPKPLQVIVD
                     DIAEVLSVTDVRKILEDYFEEGKGQDPIVHFYETFLTAYDPSIREKRGVYYTPEPVVR
                     YIVRSVNELLKTHFDMPDGLASKNVTLLDPAAGTLTFPAEAVKLAVKEFTEKYGSGGR
                     NNFIRNQVLENFFAFELMMAPYAIGHLKMSFLLEELGYKLNDEERFKLYLTNTLEMDE
                     LEQISIPGLSSLSEESHLAGKIKKEQPVLVILGNPPYSGISANVNEWTEKLLKQNIDG
                     AQSYYEVDGKTLGEKNPKWLQDDYVKFLRFAQWKIQKAGFGVVGMITNHSYLDNPTFR
                     GMRQSLMKTFNEIYILDLHGNSLKKETAPDGGKDENVFDIRQGTAIAIFVKQKEKSDC
                     KVYHAELFGKRQSKYDWLDAHQLEIKNYQLLKPESPWYFFIPRNTQNIKYYLKWKQIN
                     EIFPVNGVGITTARDNFVIDFRKEALLNKIRLFKNSKFSDNELYQFFHINKKIGWNIR
                     KAWNMLQDIPDNELENFIFDFIYRPFDHRYIFWHDSLVWRTVKQIMSHILKENLSITT
                     VRQVKSGETWQHSLVSDKLIESCYISNKTSEICYAFPLYLYNEHTPKKSRTQYMMMLF
                     EPETEYGNSNKEPNIDKKIYQTLNQNYGHELTPEEILYYIYAVFYSNIYREKYAEFLK
                     IDFPRVPFTTDYKVFSKMAALGNELVDLHLMKSSRLNTLVSKFQGEGENDVIEEIIFR
                     PEESRIYINSEKYFDNVAPQVWDYQVGGYRVLQKYLKDRKGRKMDDFVHYSQIISALN
                     YTIEIQQEIDTFYSEAEKEVIEF"
     gene            complement(18753..18839)
                     /locus_tag="PLD44_00085"
     tRNA            complement(18753..18839)
                     /locus_tag="PLD44_00085"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18803..18805),aa:Ser,seq:cga)
     gene            19137..20474
                     /locus_tag="PLD44_00090"
     CDS             19137..20474
                     /locus_tag="PLD44_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOF97770.1"
                     /translation="MKVEQVIDNLKRRFPNEPEYYQAVQEVLESIVDVYNAHPEFEKQ
                     NLVERLVIPDRILSFRVTWVDDKGNIQTNMGYRVQHNNAIGPYKGGLRFHPSVYPGIL
                     KFLAFEQTFKNALTTLPMGGAKGGSDFNPKGKSDAEVMRFCQAFMLELNKIVGPDTDV
                     PAGDIGVGGREIGYMYGMYKKLRNEANGVLTGKGLEFGGSLIRPEATGYGNIYFLMNM
                     LKTKGIHDLKGQVVAISGSGNVATYTAQKALQLGAKVITFSDSNGYIYDPDGIDEEKL
                     AYVFELKNVYRGRIKEYAEKYGCKYVEGARPWSEKCTIALPSATQNEITGEDAEMLVK
                     NGCIAVSEGANMPSTPEAVDVFLKNKILYAPGKAANAGGVSVSGLEMTQNSMRLNWTR
                     EEVDQKLQQIMQNIHEACVKYGTEADGYVNYVKGANIAGFMKVAKAMMAQGII"
     gene            complement(20541..21419)
                     /locus_tag="PLD44_00095"
     CDS             complement(20541..21419)
                     /locus_tag="PLD44_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOF97771.1"
                     /translation="MFSHIGEIAGLMVALCWTMSALFFEKAGHKIGSLTVNVIRLSLA
                     VVFLGITTFFTRGFFFPTDATHYQWFWLGLSGIIGFFLGDLFLFKSYMVIGSRTAQLI
                     MSLCPMLTALIGWFFLDEKLSLKSILGIVICVTGIIIAISNRNLKLNIPFKGFLLAFG
                     GALGQAVGLILSKKGIGNYDPVAATQIRALFGLVCFALFITFLGRWKQVSAAFTNKSA
                     LKSVTVGTFFGPFVGVALSMFAIQHTKTGIASTLMALVPVFIIWPSAVMFKERITIPQ
                     IIGAFVSIAGVSLFFL"
     gene            21650..22054
                     /locus_tag="PLD44_00100"
     CDS             21650..22054
                     /locus_tag="PLD44_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97772.1"
                     /translation="MPTEDYLLKYLEKLSRVIAAMLGFRDSGFPEDALKMADSTYREL
                     LNFDLKELSIEPTEKFIETVRKANYSASILDLLVQLTYETANALEQNHQSAEANGFYE
                     KTLELYRLLNEKDKTFSFEREMRIGEIKQKLL"
     gene            22079..22849
                     /gene="rlmB"
                     /locus_tag="PLD44_00105"
     CDS             22079..22849
                     /gene="rlmB"
                     /locus_tag="PLD44_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOF97773.1"
                     /translation="MTIYKNKRRPEKEMIFGIRPVLEAIQSGKDIDKILLRRDMSNEI
                     SRELFAATKGLHIPVQFVPLEKLNKFTDKNHQGVIAFTSPVTYQRIEDIIPAVYEVGK
                     MPFIVVLDGITDVRNFGAIARTCECAGVDAIVIPAKGGVSITSDAVKTSAGALLSIPV
                     CRENNLYETLQFLADSGIKLIAATEKGEKNYTAIDFNEPTAIVLGSEDEGISPELLQL
                     CNEKVQIPIFGNISSLNVSVAAGILIYEVIRQREQKNQ"
     gene            22902..23591
                     /locus_tag="PLD44_00110"
     CDS             22902..23591
                     /locus_tag="PLD44_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOF97774.1"
                     /translation="MEILYEDNHIIAVNKTVSEIVQGDKTGDEPLSETLKKYLKNKYN
                     KPGNVFLGVTHRLDRPVSGVVLFAKTDKALSRLNEMFQNKEIKKTYWAIVQQKPPKMV
                     DTLEHFLVRNEAKNKTTAFDRPVDNAKKAILHYKVLTSSDYYYLLEIQLETGRHHQIR
                     CQLAKIGCPIRGDLKYGFPRPNSNGGISLHARKIEFVHPVSKNWIKIVAPAPQDDKLW
                     QFFEESQKEKE"
     gene            23616..24461
                     /gene="lipA"
                     /locus_tag="PLD44_00115"
     CDS             23616..24461
                     /gene="lipA"
                     /locus_tag="PLD44_00115"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOF97775.1"
                     /translation="MNFQRKPDWIRTKLHSEATFSEVQQIIEKNGLHTICTSGRCPNQ
                     SECWNKGTATLMILGEICTRACRFCNTLTGQPLPPDTTEPERIAQSIRSLHLRHAVIT
                     SVDRDDLPDKGASHWAKTIRAVKIVNPAITLETLIPDFDGETTLLDLVIAEKPDIISH
                     NLETVSRLTPKIRSKAKYQTSLKVLQYVSSCGITAKTGIMVGLGETEDEIFSLMDDAL
                     KAGCSVLTIGQYLQPSRKNIPVHEYISPKKFEEYRQIGLAKGFRHVESGPFVRSSYHA
                     EKHVL"
     gene            complement(24668..25609)
                     /locus_tag="PLD44_00120"
     CDS             complement(24668..25609)
                     /locus_tag="PLD44_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOF97776.1"
                     /translation="MWKDFFYFSKGQQVGIIVLLVLIFVVSVVRFALPYLIPSNSNAF
                     DTNFVAEAENFKKSLLSLDSIQQAERQRQFEERYAAYRLNYRKDFSKKENHYELFNFD
                     PNTADSATFVQLGLKSYVASNILKYRSKGGSFKNAEDFSKIYGIPSEKFNELLPYIQI
                     DEKFSNPPVNLGEKAVKENPTSLVELNSADTTLLMQVKGIGKYYAKSIVRFRQNTGGF
                     FSVDQLSEIYGMTPENLEKIKPFCTVNPNLIEKIKVNTSSVDRLNKHPYINFYQAKAI
                     YELRRKKGKLHQFGELKNLSEFTEEQLKKIEPYLSFE"
     gene            26029..27342
                     /locus_tag="PLD44_00125"
     CDS             26029..27342
                     /locus_tag="PLD44_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOF97777.1"
                     /translation="MKNELLKKIEDRKIVVGVVGLGYVGLPLAVEKAKAGFKTIGFDV
                     QPQKVEMVNAGHNYIGDVVDSDLDELVKKGMLSATTDFSFVKDVDFIAICVPTPLDEH
                     QQPDISYVKSSAEAIGKFLKKGTIVVLESTTYPGTTEELIKPILEEESGLKCGIDFYL
                     GFSPERVDPGNLIYKTKNTPKVVGAIGEDALEVIAAMYRAVLEGDVFTVSSPAVAEME
                     KILENTYRNVNIGLINELAMLCHKMNINIWEVIEAAKTKPYGFQPFYPGPGLGGHCIP
                     LDPYYLSWKAREYGFHTSMIESSMMINDRMPEYCVERASKILNRSKKALNGSNILVLG
                     IAYKQDIDDYRESPALNVIDLLKSEGVNVCYFDPYIPEYLRHGKVFKGEKELTSALLA
                     KMDLVIVTAAHSNVDYDFVQQHAKYVFDTKNAMKNVKNRTNIEIL"
     gene            27350..28306
                     /locus_tag="PLD44_00130"
     CDS             27350..28306
                     /locus_tag="PLD44_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOF97778.1"
                     /translation="MNKKICVVGGGKWGQNHIKTLYQLGNLGAIVEANAERLNELLKL
                     YPVEGFTELEQALEQDFDGFTVAVPAPLHYEVAKKIIESGKSVLVEKPMTLSAETSKE
                     LVELAQRKGVQLMVGHVLLFHPAIRKIKEIIDSGELGNLYYLYSNRLNLGTVRTEENV
                     FWSFAPHDISVLDYFVGHPAEKIVSKGSKFLQEEVYDVTITQLDYPNNVHAHIFVSWL
                     HPFKEQRLVVIGSKGMVSFDDATAEKEIKFYDKRIDFEKGIPVKVENPTRIIPYEQKQ
                     PLSEELKYFIEHLDKKIEIADGQSGLEVVKVLEEAEKKIQVG"
     gene            28679..29248
                     /locus_tag="PLD44_00135"
     CDS             28679..29248
                     /locus_tag="PLD44_00135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOF97779.1"
                     /translation="MEKEYFVHESSYIDEQVKIGKGTKIWHFCHVQKGAEIGENCSLG
                     QNVNIANNVKIGNNVRIQNNVSVYEGVELEDNVFCGPSCVFTNVKTPRAHYPVHGIYD
                     KTLIKNGASLGANSTVVCGHTVGRSAMVAAGAVVTKNVKDYALVAGIPARQIGWVCEC
                     GERLEEDLVCKKCGKKYQETENGLKEIYL"
     gene            29497..30486
                     /locus_tag="PLD44_00140"
     CDS             29497..30486
                     /locus_tag="PLD44_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008822898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOF97780.1"
                     /translation="MFLIATGIFFLIKNQSSSKYIFNEGKIHGTYYHAIYMHPQGIDL
                     QEKIEERMKKIDMSLSTFEPQSVISRINRNDDSVLTDDDFETVFKTAQKISEITYGAF
                     DITVAPLVNAWGFGFDSHDRILQPNLDTIMPFIGYQKIRLENHKLIKDYPKIMLDASA
                     IAKGYSTDIAANVLEENGCKNYLVEIGGEIVCKGKNTKGEKWKIGIDKPIDDETNTLN
                     EIQTVISITDKAMATSGNYRQFYIKDGKKYSHTIDPRTGYPVNKNILSATVIAPNCIT
                     ADALATAFMVLGVDSSLMICRTLPEVDCYLIYNDQKGSNKVIYSEGFENYFSK"
     gene            30577..31383
                     /gene="bamD"
                     /locus_tag="PLD44_00145"
     CDS             30577..31383
                     /gene="bamD"
                     /locus_tag="PLD44_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOF97781.1"
                     /translation="MKKYFLVFSILIFLFSSCSEYQKLLKSTDIELKYNKAIEYFNNG
                     DFARATTLFEDISSYYRGTDRSEDILNFLGQSYLGQKDYYTASNYYQTYLKSYPKGRY
                     AENAKFLVGYCYYKDSPDPRLDQTATYQAIASFQEFLDLYPESNKVPQVNKLLDELHD
                     KLAYKYYMNARLYYNLGNYMGNNYLSAVITAQNALKKYPSSSYRDDLSFLILQAKFAQ
                     AQQSILAKKEERYQDTIDEYYSYINEFPNGKFKKQANNIFNDCKKAINLK"
     gene            31397..31726
                     /locus_tag="PLD44_00150"
     CDS             31397..31726
                     /locus_tag="PLD44_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOF97782.1"
                     /translation="MDFKKMNVPSTTITRDINLIGEKVGNIYEAVVIISKRANQISLE
                     IKSELDKKLQEFSAMGDNIEEVFENREQIEISRYYEKLPKPTLIAIEEFLEDKIYYRN
                     PIKEKLK"
     gene            32168..32536
                     /locus_tag="PLD44_00155"
     CDS             32168..32536
                     /locus_tag="PLD44_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOF97783.1"
                     /translation="MAKEVAQNSGIIYNSLTNGSKIVGTITAENDFRIDGEVEGEIFC
                     NGKVVIGQKGYLKGSISCNNAEIIGTVEGEISVQDTLSLRQTAKVTGNVKINVLIIEP
                     GAIFNGQCCMNETDSIQESE"
     gene            32610..33608
                     /locus_tag="PLD44_00160"
     CDS             32610..33608
                     /locus_tag="PLD44_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HOF97784.1"
                     /translation="MENITYKALVVEEDGNRYSRKIKELTTEHLFSYGDVLIRVYYSS
                     LNYKDALSATGNKGVTKNYPHTPGIDAAGVVIASNNEKFQSGEKVIVNGYDLGMNTPG
                     GFGEYICVPSDWIVKLPEGLTLKESMIIGTAGFTAGISLTRLTELVSPDMGEIIVSGG
                     TGGVGSMSLSMLAKLGYQTVAITGKITEKSFFETIGCHKILPRSEFESLPQKPILSAK
                     YAGGIDTVGGGILENIIKQIQPLGAVTTCGSIHSTELNISVFPFILRGISLIGVSAQN
                     YPMNLREKLWKKLAADWKPEQLLNLYTEISLPQLSEAIDNMLQGKLKGRILVNLTA"
     gene            complement(33687..34187)
                     /locus_tag="PLD44_00165"
     CDS             complement(33687..34187)
                     /locus_tag="PLD44_00165"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOF97785.1"
                     /translation="MPKISIIAAVADNYAIGNLNKLPWHLPADLKYFKKLTTGHAVIM
                     GKHTFENLPNGPLPNRKNIVLTSVLSDGICEGYFEANSIEEALELCEKEDEVFIIGGV
                     SIFKQTIDLADKMYITWIHGDFQADAYFPKINFEEWEEISREDHPADEKNPYPYTFSV
                     YQRKKK"
     gene            complement(34295..35089)
                     /locus_tag="PLD44_00170"
     CDS             complement(34295..35089)
                     /locus_tag="PLD44_00170"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOF97786.1"
                     /translation="MKQYLDLLNRVLTEGVHKEDRTGVGTLSVFGHQMRFSLDEGFPC
                     LTTKKLHLKSIIYELLWFLRGDTNVKFLQENGVSIWNEWADEHGDLGPIYGYQWRSWP
                     DYEGGYIDQIAQAVEAIKNNPNSRRIIVSAWNVADLPKMKLPPCHAFFQFYVADGKLS
                     LQLYQRSADIFLGVPFNIASYALLLMMMAQVTGLKAGDFVHTLGDAHIYTNHLEQVKL
                     QLTRTPRPLPTMLINPEVKDIFSFQYSDFQLINYDPYPHIKGEVAV"
     gene            complement(35170..37179)
                     /locus_tag="PLD44_00175"
     CDS             complement(35170..37179)
                     /locus_tag="PLD44_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOF97787.1"
                     /translation="MQFFIKKPSFILYRIISLCLLLLFLQSCGVSSRIKKADSRFELG
                     EYYAAGDLYKSSYGRIPNKDKSLRGYVAFRQGECYRLINNQRAEQAYLNAIRNNFADS
                     TVFLRYAQVLHRNGKYGEALRNYQLYLQKDSSCIIAKNGIRAIQMLEEWQKNATRYVV
                     KYDNQFNVRRASTFSPAFVNTAADELVFTSTRSTDKKATAKADPITGLPKNNLFLARK
                     NATGKWGKPELLEGEINTPETDDGVCSFAANGKTIYFTRAQQSDLVDMGTKIYVSSRA
                     GATWSPPQMLKIFSDSSISVAHPAIAPDGQTLYFVSDKKDGLGGKDIYRATLEGTDCK
                     YIENLGPEINTPGDEMFPVVRADGTLYFSSNGHPGFGGLDIFKAVPQKNGGWKVENMG
                     APVNSKADDFGMTFAGESESGYFSSNRNEMRGYDALWSFHLPELIYLVEGKVFDDQGQ
                     TISDATIRIVGSNGMNARIQTKKDGTFRIKLEKDVDYVMLATARGFLNQKNELSTQGL
                     TDSKTFNVNFKLSSISKPVKIENIFYEFGKWDLTPASEKSLQELVKLLKDNPNITIEL
                     SSHTDYIGDNAFNKNLSEKRAQSVVNYLIKAGIDKERLTAVGYGEEKPIVVDESLAQK
                     YPFLKVGETLTESYILTLTPEQQEIANQINRRTEFRVLKTTYNLY"
     gene            complement(37262..38506)
                     /locus_tag="PLD44_00180"
     CDS             complement(37262..38506)
                     /locus_tag="PLD44_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOF97788.1"
                     /translation="MAIVKPFRGIRPPREIVEKVASRPYDVLNSEEAREEAKGNPMSL
                     YHIIKPEIDFPEGTDEHDERVYAKAAENFKKFRENGWLVQDEEEHFYIYAQTMNGRTQ
                     YGIVVCAHIDDYLNGHIKKHELTRSDKEEDRMKHIRVTNANVEPVFLAYPSNEELNGI
                     VSEIVKSEPVYNFVAPDGFGHHFWKIDDKEIIHLISEIFQKIPSLYIADGHHRSAAAV
                     RVGDEKRRQNPHHTGKEEYNYFMAVCFPDNQLQIMDYNRVVKDLNQLDDEEFLQKLSQ
                     YFIVEKMGTEIYRPNKLHNFSLYLSGNWYSLTAREGTYNDNDPIEGLDVTISSKYILD
                     KILGISDLRTDKRIDFVGGIRGLGELKKLVDSGKMRVALALYPVSMQQLIAIADTGNI
                     MPPKTTWFEPKLRSGLVIHELS"
     gene            38729..39685
                     /locus_tag="PLD44_00185"
     CDS             38729..39685
                     /locus_tag="PLD44_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOF97789.1"
                     /translation="MRVLVSTRLPEEGFSELMKKFEVVFPEKEIFSKEEVIQIIPEFD
                     AFIPTFQFKVDRDVIGAGAQGKLKIIANYGVGYDNIDVDFATKCGIVVTNTPDPVVEP
                     TAEMAIALMLSVARRVSECDRKLRTDNSIKWGVMENLGISLYGKTLGIVGLGRIGKAV
                     ARRAAALGMKIIYFNRNPLDKKTETKLKARYAPLGQLLCESDVISLHVPLTESTFHLI
                     NENTLRLMKPTAILINTSRGPVIDESALTEALTNHKIFGAGLDVFEHEPTIPENLKKL
                     DNVVLSPHNGTATIEDRNAMSRFASQNILRFFEGNKNISRVN"
     gene            39745..40317
                     /locus_tag="PLD44_00190"
     CDS             39745..40317
                     /locus_tag="PLD44_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOF97790.1"
                     /translation="MENDKNIKGEEKLKEQLQDPKRKAAAFSQLVRMYQERLYWHIRK
                     MVLSHDDANDILQNTFMKAWNGIDNFRGDSLVSTWFYRIATNETLTFLANKRMKNINS
                     LDEVEDVLLQQLQADQYFNGNEAQLKLQQAILTLPEKQRLVFNMRYFDDLSYEQMENI
                     LGTTVGALKASYHLAVKKIEKFLLDSNIEI"
     gene            40422..40802
                     /locus_tag="PLD44_00195"
     CDS             40422..40802
                     /locus_tag="PLD44_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97791.1"
                     /translation="MKKEKNILKKIGNDLPFSVPENYFEQFANEIDRQIGYAKEMEVS
                     KSQKRSFAVISKSWLYMAAMFVGIFLLGNVFYLAFKNSERQKQENYEMYLLSQVDEPT
                     MMYYYMNDAYEAENGTETFQNTSN"
     gene            40809..41267
                     /locus_tag="PLD44_00200"
     CDS             40809..41267
                     /locus_tag="PLD44_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97792.1"
                     /translation="MKKFKYILMLMVWITANSLSAEELVNTNIQKMHEKKWAYIVEQA
                     KLTPDEASKVKPVFMNYENAVWELHEKNRETLNNFLKENKTQPNYELLNDEYVNLQIK
                     QAQLLKNYHLKLKKALSPEKLFHYYQAERTFKRKLLHNMPQGRKMQKNRP"
     gene            41345..41415
                     /locus_tag="PLD44_00205"
     tRNA            41345..41415
                     /locus_tag="PLD44_00205"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41377..41379,aa:Cys,seq:gca)
     gene            complement(41493..42449)
                     /gene="tal"
                     /locus_tag="PLD44_00210"
     CDS             complement(41493..42449)
                     /gene="tal"
                     /locus_tag="PLD44_00210"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HOF97793.1"
                     /translation="MNLLEQLKKFTTVVADTGDFASMMAYQPVDATTNPSLIFSASQD
                     PKYEYLMEDAIRYAQSLTSDKEEQLKKAMDKLAVNFGIKILEIVPGRVSTEVDARLSF
                     DVKGTIAKARELIALYESEGISRERILIKIASTWEGIRAAEILEKEGIHCNLTLLFSL
                     TQAVACAEAGVTLISPFVGRILDWYKKDRGVSDIPPEEDPGVKSVTAIYNYFKKFGYA
                     TQVMGASFRNMGEIIQLAGCDLLTISPALLKELEQTEGVLERKLDPEKAKTADIQPLK
                     INEISFRWMMNEDAMATEKLAEGIRNFTKDLLKLENKLAALL"
     gene            complement(42568..43758)
                     /locus_tag="PLD44_00215"
     CDS             complement(42568..43758)
                     /locus_tag="PLD44_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HOF97794.1"
                     /translation="MVSTTGKSARLLSLDVLRGITIAGMILVNNPGNWGSVYPPLRHA
                     EWNGLTPTDLVFPFFMFIMGVSMYLSYKKFDFKFSNRTFLKLFRRSSLLFLIGLAIGW
                     LGLFSGGLRELQGTDIPLGERIWQAATNFENVRILGVLQRLALVSFFGTLIILLIPHK
                     HIPCLTGAILLIYWILIGVTGSYVASENNIIAIVDRAILGASHMYTETLPDGKVIPFD
                     PEGILSTLPCIAHVLIGFWAGKLISESKSKNEQIQNLAIFGTILFFAGFFLHYGFPVN
                     KKIWSSSFVLVTCGLASLLFSLLIWIIDVNNKKKWSVFFESFGVNPLFIYVVADILSI
                     LLSNVVFMYQGNWISIKNFVYFEWLSSVFGNYLGSLIFALCFVFINWIIAHILYKKKI
                     YIKL"
     gene            43977..44375
                     /locus_tag="PLD44_00220"
     CDS             43977..44375
                     /locus_tag="PLD44_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOF97795.1"
                     /translation="MSRPRQKRIIKSPPLMMGYKPFGIPHKELDKIELHFDGFEAIRL
                     LDYEGMTQEEAAGHMNVSRPTLTRIYESARKTIAQALVEGKMIVIKGGNIEFEHQWFR
                     CKYCYQLVKEGETHVHQKYSDGNELLPIES"
     gene            44420..45166
                     /locus_tag="PLD44_00225"
     CDS             44420..45166
                     /locus_tag="PLD44_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOF97796.1"
                     /translation="MSESFLSAVVALFLGSVGSVLLAGLILLLKERQLHRVTHVLTYL
                     AGGTLLGSALLGMIPEASDMMEIKKVLYVVLAGIIFFFVLEKIILWRVCSDASCERNL
                     QTAAPMVLFGDAFHNAIDGVIIASAFLTSNTFGWVVTLAVIFHEVPQELGDFGILLKS
                     GMKRSRALLYNALSGASALIFGIVAYFSLNFAKSLIPYALAFSAAGFLYIALADLIPE
                     MHRKTSFKESMVQIFFILLGIMLIYFFGRD"
     gene            complement(45557..45982)
                     /gene="ybeY"
                     /locus_tag="PLD44_00230"
     CDS             complement(45557..45982)
                     /gene="ybeY"
                     /locus_tag="PLD44_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOF97797.1"
                     /translation="MIQYFSENIPMPALKKQKINLWIKQTAHNYEKKVGEIAYIFCNN
                     ERILEINRQYLQHDYYTDIITFDDSTDSTISGDIFISLETVKSNAEDFCVDFEEELHR
                     VIIHGILHLCGQDDITPELKAEMTRKENKALEELKKCKF"
     gene            complement(46217..47410)
                     /locus_tag="PLD44_00235"
     CDS             complement(46217..47410)
                     /locus_tag="PLD44_00235"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOF97798.1"
                     /translation="MFKVSERLAALSPSETLAMSQKSNELKAQGFDVINLSVGEPDFN
                     TPDHIKAAAKQAVDNNYSFYSPVPGFLELRKAICSKLKNENNLEFTPDQIVCSNGAKQ
                     AICNVLLTILNKDDEVIIPAPYWVSYPEMVKLGEGKSVFIYSDIENDFKISPEQLEKA
                     ITEKTKALILCSPSNPTGSVYTKEELQGLAQVLAKHPQILILSDEIYEHINYVGKHES
                     IAQFEEIRDRVVVINGVSKGYAMTGWRIGWIAAPKWIASACGTLQGQYTSGACSVAQK
                     AAEAAYTGDQSCVETMRAAFERRRNLVVELVRQIPGLKVSVPQGAFYVFPDCSAYLGK
                     SFNGKVIENSADLAMFLLETAHVASVGGVAFGAPQCIRFSYATSEDNLKKAFERIQQA
                     LNKLQ"
     gene            complement(47724..48968)
                     /locus_tag="PLD44_00240"
     CDS             complement(47724..48968)
                     /locus_tag="PLD44_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOF97799.1"
                     /translation="MNRKKVEIITIGDEILIGQIVDTNSAWMAQELNKAGFEVFQITS
                     VHDEASHITNALDEALKRTDIVLITGGIGPTNDDITKKTLCEYFHTKLVFDPSVIENI
                     KQVFANRIDFVFNDLTYSQAMVPENCTVIQNKVGTAPITWFEQDEKVVVSMPGVPYEM
                     MEAMRNEIIPRLQKKFQTQKILHKTIQVFGYSESALAIKLADWEKELPDFIHLAYLPN
                     YGIVKLRLTGTSDDLLSLEFAVNQQIESLKQLLGKAIIAFEDLPIEQIVGNLLTAQGK
                     TTATAESCTGGNIAHRFTSHAGSSEYFKGGIVAYANEVKTEVLQVSSEDLNLYGAVSR
                     QVVEQMARGALQLLHTDTAIATSGIAGPSGGTAEKPVGTVWIAACSKDKMTSRLFHFG
                     NTREQIIQRSTQAALVMLKEIT"
     assembly_gap    49172..49271
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<49272..49810)
                     /locus_tag="PLD44_00245"
     CDS             complement(<49272..49810)
                     /locus_tag="PLD44_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOF97800.1"
                     /translation="MFQVNDTLISENLLEEFFVCDLVSCKGICCVEGDAGAPLEKDEL
                     KIIKDILPEIQEFLPENSKKIIREKGFYYTDEDGDCVTSLVNERECVFAFFEEDGTCK
                     CSLEKAYYQGKTDFCKPISCHLYPVRLQKINDYTAVNLHHWKVCECAFVLGKKLKIPA
                     YRFLKVPLIRRFGEKWYKEV"
     gene            complement(49870..50361)
                     /locus_tag="PLD44_00250"
     CDS             complement(49870..50361)
                     /locus_tag="PLD44_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOF97801.1"
                     /translation="MKRIGILSDTHGYLDPRIYQYFSECDEIWHAGDWGSLEVVNQLN
                     ELKPVRGVWGNIDDVNIRSLFPQHFHFQCEDVAVWLTHIGGYPGKYDRSVRPAIFQQP
                     PDLFVCGHSHILKVQYDKKLNLLHINPGAAGKYGFHAVQTIVRLEIDGKKIQNLEVIE
                     LEK"
     gene            51287..51802
                     /locus_tag="PLD44_00255"
     CDS             51287..51802
                     /locus_tag="PLD44_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97802.1"
                     /translation="MFTTIEISAQEIGNLPEKTPEQEAMQQTSKLQQELDLNAEQVKK
                     IYEINLRYAKQRQSVTKRSEVMELLKRKDGEIENALGKEKYQQLQNKRNERISIDVPT
                     TSGMQNITPSYRSTKRTGNTNLSFPERNLQNDSKTSDGNTEQPKRLTPNNSRRTTDEV
                     ERKNTPPQQRK"
     gene            51809..52522
                     /locus_tag="PLD44_00260"
     CDS             51809..52522
                     /locus_tag="PLD44_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOF97803.1"
                     /translation="MSHPFFQFKQFTVFHDRCAMKVGIDGVTLGAWTPVDNAKKILDI
                     GTGTGLIALMLAQRSTAEIDALDIDKQAIEQARFNVENSAWKNRIRLFETSLQEYHSV
                     CTTAYDVLVCNPPYFINSKKSPIVARNMARHAESLTHEELIDLSFDLLKPDGTLCVIL
                     PVEEGLQAISHALASNWYAHQMVFVFPKPAAKAKRLLIQLGKKEKITRISRLAIETEQ
                     RHEYTDEYKNLVKNFYLKL"
     gene            complement(52543..52857)
                     /gene="trxA"
                     /locus_tag="PLD44_00265"
     CDS             complement(52543..52857)
                     /gene="trxA"
                     /locus_tag="PLD44_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOF97804.1"
                     /translation="MVIEFTDENLNEFIESGKPVLVDFWAEWCGPCRMVGPIVEELAE
                     EYDGKVQVGKLNVDENAEVPTKFGIRNIPTLLFFKDGQLVDKQVGATSKSVLAEKIKS
                     LI"
     gene            complement(52980..56771)
                     /gene="dnaE"
                     /locus_tag="PLD44_00270"
     CDS             complement(52980..56771)
                     /gene="dnaE"
                     /locus_tag="PLD44_00270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOF97805.1"
                     /translation="MNTFVHLHVHSHYSILDGMSSIDQLVDKAMKTGMPAIALTDHGS
                     MFGIKEFYNYVKKKNVPVYENISLLQKQLNKDELSDIQKDEIHKNIETEKRKLFKPII
                     GCETYVAHRSRFSKESQEDRSGYHLILLAKNKTGYRNLCKLISLAWIEGMYYRPRIDH
                     EILEKYSEGLIASSACLGGEIHKKIESGNLEEAEKAVLWYKKVFGDDFYLELQRHKTD
                     KPGGDTSTYEKQTLQNVELIKLARKTNTKLIATNDVHFVEEEHAEGHERLICLSTGKD
                     MDDPTRLRYTKQEWLKTPQQMQEIFSDIPEALENTLEIAQKVEFYDIDSAPMMPTFKI
                     PEEFADENQYRSRYTEAMLREEFGKGFDRLGGMDKVIRIKLEADYLRNLTFEGAKERY
                     GENLDPELEERINFELNVIKTMGFPGYFLIVQDFIAAARNMGVAVGPGRGSAAGSVVA
                     YCLKITDIDPLKYDLLFERFLNPDRISMPDIDVDFDDDGRNEVLRWVTEKYGKDRVAH
                     IITFGTMAAKSAIKDVARVQKLPLSEAERLTKLIPDKLPDTPDGKKMKVNLSNCFKFV
                     PELEIARNSSDENLSSTLRYAEMLEGTVRQTGVHACGIIIGADDLTKFVPLSTAEDKD
                     TKEEILVTQYEGSVIEEVGLIKMDFLGLKTLSIIKDTLENIRKSKNIEVDLDKVPLDD
                     PETYQLFSKGQTVGTFQFESAGMQKHLQALQPTQIEDLIAMNALYRPGPMQYIPQYIN
                     RKHGLEKIEYPFPIMEKRLKETYGITIYQEQVMLLSVDLAGFTRAEADELRKAMGKKL
                     TGKMQALKEKFIAGCQKNGYGPLEKLEQIWSDWVEFSNYAFNKSHATCYSYLAYRTAY
                     LKAHYPSEFMAANLSRNKDDIDEVSKFMDECRNMGINVLGPDVNESDFTFSVNEKNDI
                     RFGLGGIKGVGENAVMAIVNERHAHGKFKNIFDFVERVNLTACNKRAIESLAIAGAFD
                     GLNDIKREQFFVENGKGETVIESLIRYGNKYQADRLMSQNTLFGDMNSIEINKPEIPY
                     APAWAPLNRLNRERELIGMFLSAHPLDQFEFEMNYICNTSTGQLKDLTSLKGKQLKIG
                     GLVTNIRHSVSKAGKPYGVFTLEDYDGSYEFVLYGNNYIEFQKYMIKDMFVFIQAIVQ
                     EKYADSKYRKNTDIGGEQELELKIQKIDFFSDIKDKFVNNLKINIPLQQLTEDTVEML
                     SKIILGNKGNVNVYIQVQDQNSFNKINFFARQHRVDINPKVYRMLKTAKSEGILDFDV
                     N"
     gene            complement(56991..58250)
                     /gene="nqrF"
                     /locus_tag="PLD44_00275"
     CDS             complement(56991..58250)
                     /gene="nqrF"
                     /locus_tag="PLD44_00275"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOF97806.1"
                     /translation="MLLAIQTSTLIASLLVFFVVIFLLVVVLLVAKQYLVASGNVTIT
                     INNEKKLEVPAGGTLLSTLVNQQIYLPSACGGKGTCAQCRCQVLEGGGEILPTETGHF
                     SRKEIKEHWRLGCQVKVKNDLSIKIPESILSIKKWECEVISNKNVATFIKELIVKLPE
                     GEHMNFLPGSYIQIDIPRYELKFSEFEVEERFRPDWDKMHLWDLTCSNDEETMRAYSM
                     ANYPAEGDIIMLNVRIATPPIDRATGKFLDVKPGIASSYIFRLKPGDKVNVSGPFGEF
                     HPIIDSKREMLYIGGGAGMAPLRSHILHLLKTLRITDRKISYWYGARSKNEIFYEEDF
                     RELEKEFPNFSFHIALSEPLPEDNWTGPVGFIHSVILNEYLKFHDEPEEIEYYMCGPG
                     PMAKAVEKMLDDLGVPREMLMFDDFGA"
     gene            complement(58359..58976)
                     /gene="nqrE"
                     /locus_tag="PLD44_00280"
     CDS             complement(58359..58976)
                     /gene="nqrE"
                     /locus_tag="PLD44_00280"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HOF97807.1"
                     /translation="MESILNIFVKSIFVDNMIFAYFLGMCSYLAVSKNVKTSLGLGTA
                     VTFVLFITVPVNYLLQNYVLKEGALQWLSPQLASLDLSYLSFILFIAVIAAITQIVEM
                     AVEKFSPSLYSSLGIFLPLIAVNCSIMGASLFMQQRAFHNIGEATSYALGSGVGWLLA
                     IVGLAAIREKMEYSNVPKPLRGLGITFITVGLMGIAFMCFSGLKI"
     gene            complement(59048..59680)
                     /locus_tag="PLD44_00285"
     CDS             complement(59048..59680)
                     /locus_tag="PLD44_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOF97808.1"
                     /translation="MSTLLSKKSRQVLLGPLSKNNPITIQVLGICSALAVTAKLEPAL
                     VMGISVTIIVAFSNIIVSLVRNTIPNRIRIIVQLVVVAALVTIVSEILKAFAYDVSVQ
                     LSVYVGLIITNCILMGRLEAFAMSNSPWESFLDGLGNGFGYAIILIIVSFFRELFGSG
                     TLLGYNIIPQSFFNVGYMNNGLMMMPPMALILVACIIWVHRAKNKELQEK"
     gene            complement(59677..60402)
                     /gene="nqrC"
                     /locus_tag="PLD44_00290"
     CDS             complement(59677..60402)
                     /gene="nqrC"
                     /locus_tag="PLD44_00290"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HOF97809.1"
                     /translation="MNTNSNTYTLVYATVMVVIVALMLAFVSGALKEKQNSNIELDKK
                     KQILKSLRIDAKQNNIAQLYDQYVIKGLIVNTNGEILSYSKKEAFDIDIAKELSKKVE
                     ERRLPIFIAQVEGETKYIISLRGTGLWGPIWGYVSLNDDKNTIYGAYFSHASETPGLG
                     ANIAEPNFQRQFEGKHIFNEKNEFVSIAVMKVGKKAEGREQVDAISGGTITSKGVETM
                     LLNCIGQYEAFFRQLSTIGEIKQ"
     gene            complement(60450..61658)
                     /locus_tag="PLD44_00295"
     CDS             complement(60450..61658)
                     /locus_tag="PLD44_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HOF97810.1"
                     /translation="MNALRNFMDKIKPYFEKGGKLEKLHSVYDGFETFLFVPTTTAPK
                     YGSHIHDPVDTKRTMSTVIVALVPALLFGMYNVGYQHYLAIGQDVNFWTEFLFGFLAV
                     LPYIVVSYAVGLGIEFIGAQIKGEEIQEGFLVTGFLIPLIIPINTPLWMVAVATAFAV
                     IFAKEVFGGTGMNIFNVALVARAFLFFGYPTHMSGDSVWIRTGTALGFGGGNVVDAFS
                     GATPLGQIATASTSVPHITDTFGHSLGFWDGFVGLIPGSVGETSIIAIMLGAFVLLIT
                     EVASWKTMLSVFFGGALMGIVFNLFGPDTAAAHFPWYNHLVFGGFAFGAVFMATDPVT
                     SARTEKGKWIYGLLIGCLAIIIRVLNPGYAEGMMLAILLMNIFAPLIDFYVVDANVKK
                     RISRAQKSIQ"
     gene            complement(61717..63099)
                     /locus_tag="PLD44_00300"
     CDS             complement(61717..63099)
                     /locus_tag="PLD44_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HOF97811.1"
                     /translation="MTNVIKTTKGLDIPIDGAAQKMIERTVISDIVALVPDYYHGIIP
                     KVLVRAGDRVKAGTPVFHDKIHENLNIVSPVSGKVLAVNRGEKRKLINITIERDVVNE
                     YENFGHPSLESLSGEEIKSALLKAGIWPYIKQRPYDVIANPNKTPKSIFISSFDTAPL
                     APDYNFILKGQFADFQTGIDALAKLTPGKVHLGVKANVPSNEFLKVKNVEITEYEGPH
                     PIGNVGIQINHVDPINKGEVVWTVNVQDMLYIGRFFNKGIIDLKKIIALTGPEVIEPK
                     YYTTLPGTAVAPFINGNVHKGIPLRYILGNVISGIKVDINDFIDPYASQIFVIDEGTE
                     IHEFLGWAMPRLNKFSATKLFFTPLLQKKFFKKIFGDIKFSYDARILGGRRAIIMSNE
                     YDRVLPMDILPEYLFKAMITGNIDKMEALGGYEIAPEDVALCEFVCTSKLPLQLIVRE
                     ALDMMKKELE"
     gene            63594..65372
                     /locus_tag="PLD44_00305"
     CDS             63594..65372
                     /locus_tag="PLD44_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOF97812.1"
                     /translation="MDWKEIYLQAINWREKHIKERQFILILSFLTGIFAALAAFVLKM
                     AIHSVQSLVTESFNRESLNYLYLITPAVGIIITSLFVRHIVKDDISHGVTRILYAISQ
                     RKSILKLHNVWTSLVGSSITIGFGGSVGAEAPIVLTGSAIGSNLGKFFKMNQKTMMLL
                     IGCGAAGAIAGIFKAPIAGLVFTLEVLMLDMTMTSIVPLLISSVTATSVTYILTGDEF
                     MFQFTNYEPFAVERIPYLLMLGVVCGLVSLYFTRGMNWLENFYKKFENPYIKWAIGGS
                     ILSVLIFLFPPLYGEGYDTIASLLNNDISSVVKQSFFLGSENKPLVLLTYLVLIIVFK
                     IFATASTTSAGGVGGIFAPSLFVGCITGFVVAEILNLIGLNMPIENFALAGMAGLMSG
                     VMHAPLTGIFLIAELTGSYYLFMTLIIVSTISYLTIILFEPHSLYAMRLAQKGELITH
                     NKDKAVLTLLKMENVIETNLNVLEPDMKLGEVIKIISHSKRNVFPVVNPKNGVLLGIL
                     TLDEIRNIMFRPELYQRFTVRQLMISPPTFLNVNMPMEKVMQIFEDTGAWNLPVVDDE
                     KHYIGFVSKSTIFNSYRHVLVHFSDE"
     gene            65416..65619
                     /gene="xseB"
                     /locus_tag="PLD44_00310"
     CDS             65416..65619
                     /gene="xseB"
                     /locus_tag="PLD44_00310"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOF97813.1"
                     /translation="MTKNKPTYDEAIAELDKILAELENNSDINMDEIAQKVKRASELL
                     QICKKQLHVLDENLEKLLSELEE"
     gene            complement(65620..66279)
                     /locus_tag="PLD44_00315"
     CDS             complement(65620..66279)
                     /locus_tag="PLD44_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOF97814.1"
                     /translation="MQINISESIRQACPDLTLSVIACEVKNSETTSILWQEIEEEMEK
                     ISSTFTLENIKMRPEISATRQVYRKLGKDPNRYRPSAEALCRRICKGVGIQRVSTLVD
                     IINLISIRSGFSIGGFDAKKIQGDLRLEVGTASDIFQAIGRGILNVEGLPLFKDAEGG
                     IGTPTSDNERTKLTMETRRLLMIINGYSGEKGLQEASEQARMLLEKYAEAKEVNITLV
                     K"
     gene            complement(66284..67075)
                     /gene="pgeF"
                     /locus_tag="PLD44_00320"
     CDS             complement(66284..67075)
                     /gene="pgeF"
                     /locus_tag="PLD44_00320"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOF97815.1"
                     /translation="MRLFIQSEILQPFEEITHFFTTRHGGVSGGEYTSFNLSPYVGDE
                     EENVHRNFEILSRQLGIGTSDIIFPYQVHDCEIREIDRSFFGLSHEQRQHFLNGVDAL
                     FTQLPGVVISITTADCVPLLFYAPEKKVIAVAHAGWRGTCAGIAAKTIETLVGKFDLS
                     PEDIFVVAGPSISPSVYEVGRDVVEEFQKNDWNLSDIFSQNDGKIFLNLWKANEISLL
                     GSGLQKNNIEFLCRCTFTESENFFSARRQGILCGRMLSGIMLKQA"
BASE COUNT        21324 a        12760 c        12414 g        20634 t
ORIGIN      
        1 aatacatgtg ggtttcatat aaaaagtttt accgatcaaa agcaatctaa atcaaggaaa
       61 agcggaaaaa acacgaaacg ctgaaaaaaa tgtaattttg caaaaaatat ttgataattt
      121 tgtccaatta atttttcaag ccatgttaca caaattttct gctgcaaata tgagactttt
      181 ttaaggaatt tccacttaaa aaccaaagaa aaatgatcac aaaaaaaaga attgcgaatt
      241 ttaaatggct atcgatgatt tcactgctga tgttcatcag cattcagcat tctgtgcaag
      301 cacaaacttc gagtattcca aacgaaattg tatcggcatt gagcaatggg gatgccaatc
      361 gtttgtcggc ttatctgaat gacaatgtcg aactctttat cgaaaacaaa aatgatattt
      421 acagccgcca acaagctgtt agcattctca atgatttttt tcatcgtttc aaagtttccg
      481 acttcaaagt catccatcag ggaaacaggg ataattcgag ttttattatc ggtattcttt
      541 ccacagctac cgaaaatttc agggtttata tactgacccg caaatcggat aacaaaacta
      601 ttattttaca actcagaata gaaacatcaa atgaatgaag attacaaaca actgattccc
      661 ttatggtttg ctgaagatat aggagacggt gatcacacta cgctggcatg tattcccgaa
      721 acggcgctgg gaaaatctca actgatcatc aaagaaaacg gtgtaattgc cggcgtggaa
      781 gtggcacgag aaatttttaa gactttcgat ccaaaattga aaatgaatgt atttattact
      841 gatggaacag aagttaaaat cggcgatatt gctttcaatg tggaaggaaa aattcagtct
      901 ttgctgcaaa ccgaacgcct gatgctgaac gtcatgcagc gcatgagcgg cattgcaacg
      961 cgtaccagac agtatgtcaa agctttggaa ggaaccaaaa cccgtgttct cgatacccga
     1021 aaaacgactc ccggacttcg atttctcgaa aaagaagctg taagaattgg cggcggcgtc
     1081 aatcaccgtt ttggtttgta cgatatgatt ttgctgaaag acaatcacat agattttgcc
     1141 ggtggaatca aagaagccat tttacgtacc aaagaatatt tgaaaaacaa gggaaaaaat
     1201 ctaaaaattg aaattgaggt acgcaatttt gacgaaattg aacaggtgat ggcaattggc
     1261 ggcgtggacc gaatcatgct cgacaatttc aatattgaaa ataccaaaaa agctgtcgaa
     1321 ataatcggag gccgctatga aatcgaatct tccggcggca tcacttttga cacacttcgt
     1381 caatatgccg aatgtggcgt tgattttatt tcggtaggag cgcttactca ttccgtaaaa
     1441 agtctggata tgagtttcaa agcggatatt cccaatctta tcgaagggat tcataaaatt
     1501 ttttaatcaa ctcgaattac atttaatttt ttaaatcgat aaatggagat tattgaaacg
     1561 cctattcccg atttgttaat tattcggcca aaggtttttg aagatgcacg tggctatttt
     1621 tacgaaacgt acaatgaaaa aagttttatg gaagccggtc ttgatattca tttttgtcag
     1681 gacaatcaat ccaagtcggt ttatggggtt attcgcggat tacattatca attatcaccc
     1741 tgcagtcagt gcaagcttgt ttctgccatt cagggaacgg tttgggatgt ggcagtggat
     1801 ttgagagctt cttcccctac cttcggaaaa tggtttggta ttgaactttc tgctgaaaat
     1861 catttgcaat ttcttattcc caaaggtttt gctcacggtt tttccgtact aaccgaaaca
     1921 gctatttttg cctacaaatg cgatgcattt tacaaccctt cgctcgaaag aggaattctt
     1981 tacaacgatc ctcagctggc tattgactgg aaaattcctg aagataaggc aattgtttcg
     2041 gctaaagatg ctaaacatcc gatttttagc aaagctgaaa agaattttta agttttgttt
     2101 ttaaataaat gtttttgaac aaataaataa atcatgcaaa ctattctcat caccggcagt
     2161 aagggacaat tgggcaacga acttcaaaaa gctgcaaaac gctttccaaa ctttcgctat
     2221 atttttaccg atgttgatga gctggatatt tgcaacaaat ctgcccttga taattttgta
     2281 aaggaaaacc ggattgaaat aattgttaat tgcgcagcct atacagccgt agataaggct
     2341 gaagaggagg ttgaaaaatg ttttgccatt aatgcacaag ccgtaaaaaa cattggagaa
     2401 attgctgaaa cttatcagat aaaagttgta catatttcta ccgattacgt ttttgacgga
     2461 aaatcttaca aaccttatac cgaagagatg gaggtttctc ccgatactgt ttacggaaaa
     2521 tcgaaactgc aaggcgaaaa atttttgcaa tcagtttgta atcaatatat tgttatcaga
     2581 acctcgtggc tctattcctc ctacggaaat aattttgtta aaaccatgct gcggctcgga
     2641 aaagagagaa attcggtaaa tgtaattttt gaccaaattg gaacgccaac ttatgcagcc
     2701 gacttggcag aaatcatttt gaaaattctt tcacaaaaca attttatttc aggcatttat
     2761 catttttcca atgaaggggt ttgttcgtgg tacgattttg ctactgccat tttcaaaatt
     2821 gcccatgtta attgtttggt tcatcccatc gaaacaaaag attatccggc cccagcgccg
     2881 cgtccctatt acagcgtgct gaataaatcc aaaattaaaa acacgtttgg actatcaatt
     2941 gcccattggg aggaaagttt ggaaaaatgt atcgaaatat taaaatctga gtattcatta
     3001 taatttttcc atcctcattt ttcgtacaac aaataagttt ttcatgcttt gcgcatgaat
     3061 tattttttgc cgaaaaaaac atggaaagta ttaaaacatc ggctgttttt tcatattttt
     3121 gcatatcaaa aaattaagaa caacttttac tcaaacaagc aaaaaatata tttacgcatg
     3181 ggaagagcat ttgaatacag aaaagcaaca aaaatgaagc gttggggaaa tatggcccgc
     3241 gtatttacaa aattgggaaa acagattacc atagctgctc gtgaaggcgg ccccgatccg
     3301 gatatgaacc ctcgcttgcg cgtattgttt cagcaagcca aaaaggaaaa tatgcccaaa
     3361 gaaaatgttg aaagagctat taaaaaagct gtttctaaag aggaggccga caattataag
     3421 gaagttatat atgaaggtta tgccccacac ggaattgcgg taattataga aaccgccact
     3481 gacaatccca cacgaacagt tgcaagtatt cgcagctatt tcaatcgtta tggcggttca
     3541 ttggggactt cgggttcagt tcaatttttg ttcgatcaca aatgtgtatt tagaattgca
     3601 cccaagtcca atctttctat cgacgatctg gagttggaat tgatcgacta tggtgtggac
     3661 gaggttattc aggatgatga agaaaacaat attgtcctat atggagattt tcaacattat
     3721 tcttccattc aaaaatacct ggaagaaaac aattttgaga tattcagtgc cgaatttgaa
     3781 tggattccca aagagacaaa ggaactgaat gatgaacaaa aagctcaggt actgaaactg
     3841 atagaaaaaa ttgaagacga cgaagatgtt caaaatgttt ttcacaacat ggcggaataa
     3901 ttaatttcaa attttacaat cgaatcagtt tgattttttc cttttaataa aaatacatat
     3961 ctattcgacc attaaaaatt caattaactc ttcaattaat acttaaaaaa tggatataag
     4021 tgttttgttc cctgcattgg catccttaac atggcaacat attttgatga ttctcattgg
     4081 actaactctc atatatttag ctatttttaa agaatatgaa cccactttac ttttacctat
     4141 tggttttgga accattctgg taaatattcc actgagttcg gctattaatc aaatagatgg
     4201 caaaaccgga gaaattattg aaggtatttt gtctgttttt tatgaaggag gaattgctac
     4261 cgaaatgttt cctttgctga tttttattgc aattggggct atgattgatt ttacaccttt
     4321 gttcaacaat ccgcatttgt tgcttttcgg agcagcagca caatttggta tttttatgac
     4381 catgttcata gccaccttat tcggattcga cctgaaagaa gctgcttcca taggaattat
     4441 tggcgctgcc gacgggccga cttccatttt tgtcgcttcg cgttttgcac ctcatctttt
     4501 gggccctatt tccgtcgcgg catactcgta tatggcgttg gttcccatta ttcagcctcc
     4561 ggttatcaaa ttgctgacgt caaaaaaaga aagactgatt cggatgcaat acagttccaa
     4621 aaaagtttcc cggaaggcat tgatcgcttt ccccattatt gtaacaatca tttcaggctt
     4681 aattgcccct gcttctcttt ctctgatagg atttttaatg tttggaaatc tgattcggga
     4741 atgtggcgta ctcaacaaat tgtcgcaatc ggctcagaat gaactggcaa gtttggtaac
     4801 catattattg ggtatcacca tagccagtac catgacggct gaacgtttcg taacatttgg
     4861 aaccctcacc atcattgctt tgggattgct ggcttttgtt ttcgatactt ttggtggagt
     4921 aatattcgcc aaacttttaa acctgatttt gccggctaag aagaaaatga atccaatgat
     4981 tggggcttgt ggaatttctg ctttcccaat gtctgcaaga gtaattcata caatgggttt
     5041 gaaggaagat ccacacaatt tcctgctgat gcctgctgtc agtgcaaatg ttggcggtca
     5101 aattgggtcg gtagtagcag gtggacttat acttactttg gtaaatttat tcgcttaaaa
     5161 tattgaacct atgatcaata atgttgaagc tgctctcgaa ataggagcaa tcggtattac
     5221 aggcatattc atttttatgc ttatatttta tgccgctatc aaacttattt tgaaactttt
     5281 ccctgaaaag gaaaacaaag aagaatagga agaaattaaa tcgatttttg attttttcga
     5341 caaccagata tttagtaaaa acgcatttta agagttattt ttaactgctt aaaatgcgtt
     5401 tttttcaatg gaataaatta gtaaaggact aatattgttc tttttcgttg ggaaaatcgg
     5461 aagattttac atccgcaatg taatgttgaa tagcctgatt catttgttct cttaaattgg
     5521 catatcttct caagaaacgt ggagaaaagc cctgcgtaag tcccagcata tcgtgaatga
     5581 ccaacacttg cccatcaact ccaccgcctg cacctattcc aataaccgga atagagagtt
     5641 cggaagcaat ttgagttccc aactttgcgg gaattttttc caacaccaaa gcaaaacaac
     5701 cggcatcctc cagcaaatgc gcatcccgta taagtttttc ggcttcttct tcttcctgtg
     5761 ccctaacatt gtatgaacca tatttgttga tggactgtgg cattaatccc aaatgcccca
     5821 taatgggaat tccggcacca ataattcttt ttaccgattc aatgatttct tcgccacctt
     5881 ccatctttat tccgtcagca tgagtttctt tcataatacg aatggctgaa gcaattgctt
     5941 cgtgagaatt tccctgataa ctgccaaaag gcatatctac gacggcaaga gctcttttaa
     6001 ttccgcgtat ggccgatttt gccagaaata tcatctgatc gagcgttatg ggcaaagttg
     6061 tggtatttcc gcaaataaca tttgaagccg agtctcccac taaaattaca tctattcctg
     6121 cttcatccac aatattggcc attgtgtaat cgtaagctgt aagcatactg attttttcac
     6181 cccgctgttt catttcgaat aaccgacgcg tggttacttt tctgttgtct tcattgtgta
     6241 ctgacatgat aatttgattt gaatttgctg caaagttaca tcaaaatttg gttttctgtc
     6301 tttttatttt attgctgaaa ggaaaagaaa ccgatgataa aaatttcaac gaaaaacaat
     6361 tttttttacc tttgcattca aacaatttta gtgaaacgat atgttctgtg attttgagtt
     6421 ttgttttagc tttgaagtgt ttcaaaaaaa attactggat aaatgagtaa aattaccatt
     6481 cacagttacc aagaactgga agaatatgtt gggaaagaat tgggggtatc ggattattta
     6541 accatcacac aggaacaaat caataaattt gccgatgcca cactggatta tcaatggatt
     6601 catgtgaatg ccgaaaaagc aaaacaggaa agtccttata aaaacactat tgcgcatgga
     6661 tatttgaatc tttccatatt accttatctt tggggtcaga ttatggaaat ccgaaattct
     6721 aaaatgacgc tcaattacgg aattgagaat gctcgttttc aacaggcagt ggtagtaaac
     6781 gatcaggtga gagccaaagc aaaattaaaa tcgctggtca atttgaaagg aatagccaaa
     6841 gcggaaattt acactgttct tgaaattaaa aacagcaata aaccggcttt ggaaggaact
     6901 ttcatttttt tatatcattt tgaataactc atatatcaga gaaacgcaag aattaaaatt
     6961 tgtgccgata caactcttag aaacatggtt aaaggataca ccgtagaata agccacagcg
     7021 gggatatccg aaccggcaat tgaattggaa taggcaagtg ctggtggatc ggtcgtacta
     7081 cctgcaagaa gtcctatcaa agtaaaataa tttatcttta agaatttccg gccaattatg
     7141 cccacaataa gcagtggaag caaagctatg gctgctgcac atcccatcca aagcaaacca
     7201 ttggcagaaa taaccgtttc gacaaatttt tctcctgctc ctatacccac actggcaaga
     7261 aacaaagaaa ttcctatttc acgcaacata taattggcac ttgtagtggt gtaggttatt
     7321 aatttcaact tgtacccaaa acgtcctaac aaaatagcca caatcaaagg gccacctgcc
     7381 aatccgagtt taaccgaaac cggtatgcct ggaacaaaaa atggaatact ccccactaaa
     7441 acacctaata aaattccgac aaaaatagtt atgataggtg gttcgttcaa tttttgcaag
     7501 gtattgccca ataatttttc cactttttca atggcgtcca attctccaac taccattact
     7561 ctgtctccta cctgtaatac caaatcgggt gttcccaaca aatcaacacc tgaacgattt
     7621 acacgggtaa tatttacacc gtaaattgta cgtaatttca gcgagccgat agtcttcccg
     7681 ttgatttttc cgtgcgtaat cacaattctt ctggaaacca gtttttcttc ggatattttc
     7741 caatccattt ccactttttc tccaatatag gatgttatgc gttcaatatc tcgcgaagaa
     7801 gcaactatca gcaaaatatc gtctttgtat aacatggaat cgccttgagg aataaaaaat
     7861 tctccattcc gcattaaacg cgaaacaata aatggccttc caacagcccg tttcacatcc
     7921 gtaactgtat gattaaaaac cttttcattt tttacctgta cagaaatttt ttcgacctgt
     7981 tcttcgtcca cttcactggc tttttttatt tgttcggttt ccttgtgaaa atccacttta
     8041 aaaattattc gcagtaatac cattgaaaaa ataattccca atactcctat cgggtaagct
     8101 accgcataac ccaaagcaat tggcggaatt tctgcacccg gtgaagatcc aaaaacatgc
     8161 cgtaatgctt cctgagcggc tcccaagccc ggagtattgg taaccgaacc ggaaagtatg
     8221 ccgaccagca tgggcatgga cactttacca tgaagaagat aatatagggc tatcactatt
     8281 cctacaccca gcagcacaat catggtcgac aataaattga gcagcatccc accttttttg
     8341 aaagaagaaa aaaaagatgg tcctacttga aggccaatgg aataaacaaa caaaatcaaa
     8401 ccgaaatttt taataaactc caaaacttcc ggattcacgc ttaatcccaa atgacctaca
     8461 aataaaccta caaaaaggac aaaagtaact cccaacgaaa tcccaaaaat tttaattttt
     8521 cccaaaagga tacccaaaga aatgacaaaa gcaaacaaca aaatggtatg tgcaatacca
     8581 tcattcaaca aaagcgaaag gaaccactcc ataaaattat taaaaaattg attttcaaac
     8641 aaataaatta aaattgtcct tattgaaaaa tgaaagcaaa tttaaacaat tttcagcatt
     8701 ggataaaata tatattcaat tgcttctaaa tttgatcgaa cacttcaata tacttaaaat
     8761 gaaacagata aacaaataat tgtagcattc gttttttatt cagacaaaaa aaattacttt
     8821 tgcattaaaa atagtggtac atttcagaaa taaggtacat tacaagcata tcaataaaat
     8881 aaacatggaa gaaaatactg gtaaaaccgt aaaaaaaaga gtgcttttta tcgatcggga
     8941 cggtaccatt atagttgaac cttctgaaac ttttcaagtt gacagcttgg aacagctcga
     9001 atttttgccc ggcgttatcc gcaatttata ttttattcgt cagagaaccg attttgagtg
     9061 ggcaatggtg tccaatcagg atggtttggg gacttcggct tatccggaag aaaatttcga
     9121 aaaagttcaa tccaaaatgt tgaaaattct cgaaaacgaa ggaataattt tcgacaaaat
     9181 ttttattgac aaatctctgc ctgaagagaa attgccaacg cgtaaacccg gaactgccat
     9241 gttgaaggaa tatttttcgg caaattatga cttggaaaat tcgtatgtgg ttggcgacag
     9301 aataaccgat gtggaattgg caaaaaatct cggctgcaaa gcaattcttc tcaatgaaga
     9361 tcctgaaatt ctgcaggaat cgaagttgaa aaaagtctgc gtgttgcaaa caacctcttg
     9421 ggacgaaata gctgcattta tctttgcagg ggaaagaacc gccaccatac aacgaactac
     9481 gaaagaaacc gatattttta ttcacctgaa tttggatggc tcaggaaaag gaaatatttc
     9541 taccggtttg cgttttttcg accacatgct cgaacaaatt gcccgtcatt cgggttgcga
     9601 cttgaccatt caagtgaaag gagatttgga agttgacgaa catcatacca tcgaagatac
     9661 cgctattgct ttgggagaag cttttgccaa agcattggga gacaagcgcg gaatagaacg
     9721 ctatggattc tgcttgccaa tggatgatag tctttgtttg gttgccatcg attttggagg
     9781 acgagcatgg ctgactttcg atgccatttt taaaagggaa tatatcggcg attttcctac
     9841 cgaaatgatt tttcactttt tcaagtcgct gagcgatgcg gcaaaaatga atctttacgt
     9901 aaaagcactt ggcgaaaatg aacatcacaa aatagaagga attttcaaag cattggccaa
     9961 agctattaaa atggctgtaa aacgagatat ttaccaattt gaacttccta caaccaaagg
    10021 aattatctaa tttttaactt atcgacctat cattattgat catggatttt aaaagaaatt
    10081 ctgcaaaatt tttctttttg atttttttac ttatcgttca atttccactc cacgcacaaa
    10141 tttttcctga ccagccgcgt ttggtaattg gtattgtggt ggacggatta caggaaaaac
    10201 acatcgattt gctttggcaa cgttttgatg caaacggttt taaaaaaatc attgaaaaag
    10261 gtactgcctt tgaaaatatg gaatataaca ttgtgtcggc cggaaatgct tcggatattg
    10321 caaccctgat gaccggcact acaccttatt atcacggagt tacaggaaat aatttctatt
    10381 ccagaaaaca gggaaatgtt caatctgtga ttttcgacga aaacgaaccg ggcataggaa
    10441 caaaacaaac cgtttctgcc catcaacttt tggccagtac cattacggat gaattgatgc
    10501 tgacctatcc gaaacaatca aaaacttatg ctatcgccat caatgctgaa gatgccatta
    10561 tgttgggtgg acatacagcc aacagcgcta tgtggctcga cgacatcaac atgaaatggg
    10621 taaccacttc gtattatacc gatggtttgg ctgccagcgc cgatgaaatg aatgtgaacg
    10681 gacaaatcga acaatatgct tcgtccgttt gggaaccgat gtactcgccc aatacttatt
    10741 tgtttccttc agcgaacgaa aacaaaaaaa acggatttca gtattttccc aacagtaaaa
    10801 gaaaaggatc caacctgccc attttaaaaa atacgcccaa tgccaatgct ttggttgccg
    10861 atttggcttt aaaaattttt gaaaaggaaa atctgggaca ggatacttat cccgatatgc
    10921 tgctcttgca atttaacgta aaaactccct atgaaaaaac atttgcgcta aaaactgccg
    10981 aaaaggaaga tatgtatttg cgcttggaca aagatttaca atatttattt caaaaaatcg
    11041 acagcaaaat aggcttgtcc aacacgttaa ttttcattct gggtacccaa acctctgctc
    11101 atacaccttt agaattgggc gaaaataaaa ttcctgcagg atattttaac ggacgtcgat
    11161 cgatggcttt gctcaacact tatttaatgg tgctttacgg acaggaacgt tgggtggaag
    11221 gttattacgg aaaaaacatt tttttgaatc gacagaaaat cgaagaaaaa aaaatcaatt
    11281 tgcaggaaat tcagcaggta gttgccgatt tcatgcacga gtttgaagga atccagtcgg
    11341 cttacacttc ttatcagatt gttaatctta ctgatccatc caacagggaa atgtcgaaac
    11401 tgcgcaattc atttcaaaaa agcagcaagg gagatgtgat cattactttg cttcccggct
    11461 ggctcgagct cgacgacaac aacaatccca taggtgagtc gaatgcggtt atttcttcaa
    11521 caccagttta tttctacgga tggaacatcg aaccaaaaaa aattgcagca atccattcga
    11581 ttattgacat agctcctacc atttgcaaaa ttttgaaact tccttttccc aacgcaacta
    11641 ccggaaaacc tttggaagaa attatcaaca agtaaaaatt tttttagcag ataatgaaaa
    11701 aatacgattt cgacgaaatt atcgacagaa cgaacaccaa tgccgttaaa atagaagctt
    11761 gtcggtcaat gtttggaacg tcgaacgtta ttcctttgtg ggtagccgat atggattttc
    11821 gtactcccga tttcattttg gatagtatcc gtaaacgctg tgaacatccc attttgggat
    11881 attctgttcc tcccaaagat tattttccat ccattatgaa ttggctgaaa gaaaatcatg
    11941 gctgggaagt taaaaaagaa tggctgggat tcatgccggg tgtggtgccg ggactttctt
    12001 ttgccgttca gcaattttct gccgaaaacg atgaaattgt tattcagcca ccggtatatt
    12061 ttccttttat gcatgtggta gaaaaaaaca acagaaaatt gatattcaat ccgttaaaag
    12121 aggttgacgg aaaatttcag atgaattttg acgatttgga gaaaaaaatt acttcaaaaa
    12181 caaaaatgtt gattttgtgt aatcctcaca atcccggagg aagaagttgg ggaaaagaaa
    12241 ctttgcaaca attggctgaa atttgtgcca aacatcatct gatagttgtg tcggatgaaa
    12301 ttcatgccga tatgacgttg cccggaaatg tgcatgtgcc ctttgcttcg gtatcggaac
    12361 gagcagcaaa tatcagtttg acttacatgt cgccaagcaa aacattcaat atcccagcgc
    12421 tgcaaacttc ctatttcatt attcccaatg aggagcttcg aaatggattg aaaaattttc
    12481 tcgatcgttc ggaactcgac aacggaaata tttttgctta cctctctacc attgccgcct
    12541 acacggaagg aaaatggtgg aaaatgcaaa tgcttgatta tgttcaggga aatatcgatt
    12601 ttgtcattca atttttgcag aatgaaattc cacaaatcaa acccatgaaa ccagaagctt
    12661 cctttttaat ttggctcaac tgttcagatt tgggtatgaa cgaagaagaa ttattccaat
    12721 tttttgtaca caaagccgga ttgggtttaa atcagggcat aactttcgga cccggaggtg
    12781 aacagcatct acgactgaat gtggcttgcc ctcgaagtac cttagaaaaa gcaatgaatc
    12841 aattgaaaaa cgcagttaga aatctataaa tcaatatcta tgtggggaac tctggtaaat
    12901 gcactggccg tgattgtggg tggaagtgcc ggtttgttgc taaataaaaa aatgcctcaa
    12961 cgtatccaaa ctatttattt tcaagtaatt ggacttttta ccattggaat cggaattaca
    13021 atggtagttg gtatgaaaca tattttgctg attgttatga gtttggtgat tggtgttttg
    13081 attggggaat ggatgcattt ggaaagcaga ctggaaaaat ggagtgaatc actgaaaaag
    13141 cgttttcaaa ttggcagtga gcaattttcg gaagggtttg ttacggcatt tttattgttt
    13201 tgcattggat ccatgactat tttgggatct attcaggaag gactgaccgg ttcgccagat
    13261 ttgctgttca ccaaatcgct aatggatgga ttttctgcca ttattctggc ttcggctttt
    13321 ggtgtcggag taattttttc ttccattccg ctccttattt ttcaaggtgg acttacactg
    13381 ctggctatgg ctgcatcttc tttttttact cccgatttaa tcaacgggat ttccaacatt
    13441 ggcggaattt tactgattgg tttaggaatc aatatcctcg aaatcaaaaa gcttgccatc
    13501 accaacatgc tcccttcact cgttgtagtt atccttttaa tatggggttt tgcataaaat
    13561 tcgttgtaaa ttaaaaattt tcgctcctga atttcacttt caatgaagaa aaaagcccga
    13621 gacaacccgg gcttaatttt tttactacaa ataaaaaaca taatttcgga aaaaggctat
    13681 ggttgctgct atgataacca aaaccattga aagcaaaatg gctgcaataa tcagaacagc
    13741 ccaaataacc agctgcagag cattggaacc ggtatcgtat tcggttgttt tactaccgtc
    13801 gctccatgtt ttctcggtgt aataatctac tgccactgcg gatgctgctg ctcccatgat
    13861 aaaagcaata attttgccga aagctccacc cgaattatgt tttcgatttg caaccacata
    13921 attgggtgca aaattggaaa gtatataagc tatggtcggt aaaaacatca ccaccgctcc
    13981 tattgtgcct ttggtatcga gatgattttt tgcaagcaga aaatatacca ccggaaaaat
    14041 cagcgataaa actgccaaaa accagtttcc ataaaatttt cgtttgctga gtttgttgag
    14101 caagcctccc aaatcattca tacggtcaat tacttcatta tcggtcggtt gataagattt
    14161 tgccatgttt aaggcacgat aggtagtttt aaaatgtttt cgtttggttg gcgaacggaa
    14221 aatctgtccg ttttcttcac tgacgggttc ccaagtatca aaagcttctt ccacacattc
    14281 cgaagccaac cgattggcat cctccttttc actgtcgata cgggcatttt tgctgtcggc
    14341 actgaaggaa gtattatttg cagtttttat cggtttgtat cttttttcgg ttataagtac
    14401 cacaatcata taaatgaccg aaacgaaaag gaaaataatc aatccggcaa gcaaaacttt
    14461 ctgaatagtt tcatttcgtt ccattctctt ttgtgcatca gcaaaggaaa ttccactaat
    14521 cgacgacaac tcatcggaag aaagcgcctt gttgtaaacc cgaatttcgt ccacctgtcc
    14581 tttaaaagcc cggttgtatg gcgaattttg cgtgtttcca ataatcagga aattgctttt
    14641 ttttgtaaaa actaccggtt tcgattcgta gtattcgtca ttgacataaa gccgtaaggt
    14701 agaatcattt gcgttggccg tcatggcaat aaaattccat ttgtctttct ttaatgtcgc
    14761 acttcgaagc gatgcaaaat agggattttt cagagaatcc aatttattga atccacatat
    14821 tacggcacct ttagaaattt gaagcgatcg ggaactgaat tttttttcat tatttaccgg
    14881 aagtgtcgag ccaaaaagaa atccatctgg acgtccgaaa acccatgccg tataggtaat
    14941 ttcgggaagg ttttgaggtg aaatatccag atgtgtttta agtccttgag catttccatc
    15001 aaataccaca gccgtgctgt ctttaccatc cttgccagtc gtccatttgt aatagtaatt
    15061 gccgggttgg cttttcaatt ttaagtgatt attatcggaa accgaatttt tgccgtttcc
    15121 attcaattcg taaaaaagaa tcagatgaga cgacagcgtg tcctgattct gggaaaaaat
    15181 tgcctgcatg caaaaaagca tgagcgtcag taaaacagtg attttcgttt tcatggttta
    15241 aatttaattg ttaaaaaaat gtttgcattt tgcctgtaaa caaacaaaaa aacaatttat
    15301 ctacacgcag tttcttattt gaattgattt ttcatactga cttacaaaaa caacactaat
    15361 tttcaaattc tgtcgaaatg ccctctcaca atcaagaaaa ttttcaaaaa gctgtcatat
    15421 caaaattcga ttacctcttt ttcagcttca gaataaaaag tatctatttc ctgttgaatt
    15481 tcaatggtat aattcaatgc cgaaattatt tgcgaataat gaacaaaatc atccattttt
    15541 cttccctttc tgtctttcag atatttttga agtacccgat aacctccaac ctgatagtcc
    15601 caaacctgag gtgctacatt atcgaaatat ttttccgaat tgatgtaaat ccgactttct
    15661 tccgggcgaa aaattatttc ttctataacg tcattttcgc cttcgccctg aaatttggaa
    15721 acaagcgtat tgagtcgact gcttttcatc aaatgtaaat ccaccaattc atttcccaaa
    15781 gcagccattt tgctgaaaac cttgtaatca gttgtgaaag gcacacgtgg aaaatcgatt
    15841 ttcaaaaact cggcatattt ttcacgataa atattgctgt aaaagacggc ataaatataa
    15901 tacagaattt cttcgggcgt aagttcatgc ccgtagtttt ggttcaatgt ttgataaatc
    15961 tttttgtcga tattgggctc tttgttgcta ttcccgtatt cggtttccgg ttcaaacaac
    16021 atcatcatgt attgcgtgcg ggattttttt ggagtatgct cattgtaaag ataaagggga
    16081 aaagcataac aaatttcgct tgttttattt gatatatagc aactttcgat taacttatcg
    16141 gaaactaaac tatgttgcca tgtttctccg gactttactt gtcttactgt cgttattgaa
    16201 agattttctt tcagtatatg agacataatc tgcttcacag ttctccaaac caatgaatcg
    16261 tgccaaaaaa tatatcgatg atcaaatggt cggtaaataa aatcaaaaat aaaattttcc
    16321 aactcattat caggaatatc ttgtaacata ttccatgctt tacgaatgtt ccaacctatt
    16381 tttttgttaa tatgaaaaaa ttgatagagt tcgttatcac taaattttga gtttttaaaa
    16441 agtcttattt tgttcaaaag agcttctttt ctgaaatcta taacgaaatt atctctggca
    16501 gttgttatgc caacaccatt tacaggaaaa atttcgttta tttgcttcca tttcaaataa
    16561 tatttgatgt tttgcgtatt tctcggaata aagaaatacc acggactttc aggtttcagc
    16621 aactgataat ttttaatttc caattgatgg gcatccagcc agtcgtattt actttgccgt
    16681 ttgccaaaaa gttcggcatg ataaaccttg cagtcggatt tctccttttg ttttacaaaa
    16741 atggctattg ccgttccctg acgaatatca aaaacatttt catcttttcc tccatccggc
    16801 gctgtttctt ttttcagact gttgccatgc aagtcgagaa tgtatatttc attgaaagtt
    16861 ttcatcaaac tttgcctcat tccgcgaaaa gtcggattat cgagataact gtggttggta
    16921 atcataccca ctacaccaaa accggctttt tgaattttcc actgagcgaa acgcaaaaat
    16981 ttgacataat cgtcctgtaa ccatttcgga tttttctctc cgagcgtttt gccgtccact
    17041 tcataatagc tttgagctcc atcaatattt tgtttaagca gtttttccgt ccattcgttg
    17101 acattggccg aaattccgct gtaaggagga tttccaagaa ttaccagaac gggctgttcc
    17161 tttttaattt tgcctgccaa atgactttct tcactcaacg aactgaggcc gggaatggaa
    17221 atttgctcca actcatccat ttccaacgta ttggtcaaat aaagtttaaa cctttcctca
    17281 tcattgagtt tgtaacccag ctcttccagc aaaaaactca tttttaaatg tccaatggcg
    17341 taaggcgcca tcattagttc aaaagcaaaa aagttctcca aaacctgatt acggataaaa
    17401 ttgtttcgtc caccactccc atatttttcg gtaaattctt ttacagccaa ctttacggct
    17461 tcagcaggga aagtaagcgt tcctgctgcc ggatcaagca aggtaacatt tttggaagcc
    17521 agtccgtcag gcatatcgaa atgcgttttc aaaagttcgt tcaccgaccg cacaatatat
    17581 ctaacaacag gttcaggcgt atagtaaaca cctcgttttt cacggatgga aggatcgtaa
    17641 gctgtcaaaa aagtttcgta aaaatgaaca attggatctt gccccttgcc ttcttcaaaa
    17701 taatcttcca gaattttcct tacatccgta acgctcaaaa cttcagcaat atcgtctaca
    17761 atgacttgta aaggtttggg tggatcttcc agtgaaataa atcgaaaaac gtcacgtaga
    17821 atgccaatgg tattgggaat atatttgaaa gcgagctcgc gattgaagga attatcagaa
    17881 cgcgtacggg cagcaaacaa tccgtacgtc aatgtttgag aatacaaatc ggcaaaagct
    17941 tcttttgtca aagttccaat cagatatttc cgaaaagctt cataaaaacc aagaatagct
    18001 tttttaccac gtttttcttc ttcatcgagt tcgatactta ccacttcatc acgcaagaat
    18061 cgggtgcgtt ttgccaactc tgttgccagc gattgagcat tgcgtacgtg aggcagcgaa
    18121 aaggaaaaaa acgtatcgag caaatcgaaa aaaaccgttt cgttttctac cggaggagca
    18181 gtttttagct ttcttgccaa ctcaggtcgt ccaaccagaa cttgttttat caactctcca
    18241 tttcgataaa gccgaaactc gtaaaaattg gtaaggataa cattcggaaa agtttcgcgg
    18301 taacgtttca actggcctga aacttcgatt ctgtcaagat ttgtaacaga agggtctttg
    18361 gcttcaatat agccggtaat atgatttttc ccgtcccaaa tacgaaaatc gggattgccg
    18421 gcttcggttg gtttgggtaa aacagtaatg tcaaccttat ttgtctgacg ttcatcagta
    18481 taattcagca acaaatcttt caaatgcccg taataactct cttcacgtgc atcaccttga
    18541 ttaaaagtcg cttgaaggct gtttaaatac tgtttcagca taaaataaat aacatgggaa
    18601 aaacacaatt aaaaaactca atcactcaaa gagattggaa aaaaacatgt gtaaaagaaa
    18661 tgaaatatag cgtgttgctt gtaaagacaa agaaatgaag agaaaaatta gggtttcatg
    18721 aaccactaaa ataaaaaact tttgaaaaaa agcggaaagt gagagattcg aactctcggt
    18781 acagttaccc gtacaacggt tttcgagacc gtcccgatcg accactccgg caactttccg
    18841 gtcaacaaaa ttttatgttg caaaaataca gttttttttc taatattaaa gaaaaatctc
    18901 ggacaaaacc aaaaaaatca ttttcataaa atataaatac tgtcgttttg ataggataat
    18961 tttaaattta acaacaatac aaataaattt cttccgaaaa aaataaaaat gtcaacagtc
    19021 atcataagaa aaataataaa aaatattgca aaatttccgc tttttgatat acagatatta
    19081 ctattttgtt ataactttgc aaccgaaaac tataaaaaat aaaaaacata aacaaaatga
    19141 aagtagaaca ggtaattgac aatttgaaaa ggagatttcc caatgagccg gagtattatc
    19201 aggcagtaca ggaagtattg gaatctattg tagatgttta caatgctcat cccgaatttg
    19261 aaaaacaaaa tttggtagaa cgcttggtta ttccggacag aattttatct ttccgcgtaa
    19321 cttgggttga cgacaagggc aacattcaaa ccaacatggg ttacagagtg cagcacaaca
    19381 acgccattgg accttacaaa ggtggtttgc gtttccatcc ttccgtatat cccggaattt
    19441 tgaaatttct tgcttttgaa caaactttta aaaacgcctt aactactcta ccaatgggtg
    19501 gagctaaagg tggttccgat tttaatccca aaggaaaatc ggacgctgaa gtaatgcgtt
    19561 tttgccaggc atttatgctt gaattgaaca aaattgtcgg tcctgatacc gatgtacctg
    19621 ccggcgacat aggcgttggc ggacgcgaaa tcggttatat gtacggcatg tacaaaaaac
    19681 tcagaaatga agccaatggt gttcttacag ggaaaggtct cgaatttgga ggttcactga
    19741 ttcgtcccga agccaccgga tacgggaaca tctacttctt gatgaatatg ctgaaaacaa
    19801 aaggtattca cgatctgaaa ggtcaagtgg ttgccatttc cggttcagga aacgttgcaa
    19861 cctacactgc ccagaaagca ctccaactcg gagccaaagt tattactttt tccgacagca
    19921 acggttatat ttatgacccg gatggcatcg atgaagaaaa actggcttac gttttcgaac
    19981 tgaaaaatgt ttatcgtgga cgtattaaag aatatgccga aaaatatggc tgcaaatatg
    20041 ttgaaggtgc acgtccctgg agcgaaaaat gtactatcgc tttgccttcg gctacgcaaa
    20101 acgaaataac aggcgaagat gccgaaatgc ttgttaaaaa tggttgtatt gccgtttccg
    20161 aaggagccaa tatgccttca actcctgaag ccgttgatgt gttcctgaaa aataaaattc
    20221 tttatgctcc gggtaaagcg gccaatgccg gtggagtttc cgtatcagga ttggaaatga
    20281 ctcaaaattc catgagattg aattggacaa gagaagaagt ggatcaaaag cttcagcaaa
    20341 ttatgcaaaa tattcacgaa gcttgtgtaa aatacggaac tgaagccgac ggatatgtaa
    20401 attatgtgaa aggtgcaaac attgccggat tcatgaaagt tgcaaaagcc atgatggctc
    20461 agggcatcat ttaagcaatt tttttaaaaa acaggaaaag aggttctccg ataattttcg
    20521 agaacctttt tttattctac tcaaaggaaa aacaagctga cgcctgctat gctgacaaat
    20581 gctccgatta tctgtggaat ggtgattctt tctttaaaca taactgccga aggccaaatg
    20641 ataaacaccg gaaccagtgc cattaatgtg gaagctatgc cggtttttgt atgttgaatg
    20701 gcaaacatgg ataacgccac gcctacaaac gggccgaaaa atgtgcctac cgttaccgat
    20761 tttaatgcgg atttgtttgt aaaagcggct gagacttgct tccatcgacc caaaaaagtg
    20821 ataaataaag caaaacaaac tagtccaaac agggctcgta tttgagtagc tgcaacagga
    20881 tcgtaatttc caattccttt tttgcttaaa attaatccta ccgcctgtcc caaagcacct
    20941 ccaaaagcca gtaaaaaacc tttaaaagga atattcagct tcaaattccg atttgaaatg
    21001 gctatgataa tccctgttac acatatcact atgccaagaa tacttttcaa cgaaagcttt
    21061 tcatccaaaa aaaaccaacc gattaaagcc gtcaacatgg gacacaagct cataatcaac
    21121 tgtgctgtac gcgaaccaat caccatatag gatttgaaaa ggaacaaatc gcccaaaaag
    21181 aaacctataa tacccgaaag tccaagccag aaccattgat aatgtgtagc atcagtggga
    21241 aagaaaaagc cgcgtgtaaa aaaagtagtg atacccagaa ataccactgc caacgataaa
    21301 cgaatcacgt tcaccgtaag tgaaccgatt ttatgaccgg ctttctcaaa aaacaaagca
    21361 ctcattgtcc aacagagcgc taccattagt cctgctattt ctcctatatg ggaaaacact
    21421 tttttttaaa tttatgattg ggttaaacaa tattttttgc aaaaaatttt tgcaaagata
    21481 attcaaaaaa actcaggtcg ttcataattt gtaaatatgc tcctatcttt ttacttgtca
    21541 acgaaataac ataattattt ttgttcgcat aaaaaagaaa tgcaattatt tggctaactt
    21601 tgcaaaaaat tttggatttt attgtttttc aaaactgatt acttcaaaaa tgcccacaga
    21661 agattattta ttgaaatatc tcgaaaaatt gagccgcgtc attgcagcca tgttgggatt
    21721 tcgcgacagc ggatttcccg aagatgcatt gaaaatggct gacagtacct atcgcgaact
    21781 tttaaatttt gatttgaaag aattatcaat tgaaccgaca gaaaaattca tcgaaactgt
    21841 tcgcaaagca aactattcgg cttccatttt ggatttgctg gttcaattga cctacgaaac
    21901 tgccaatgct ttggaacaaa atcatcagtc tgccgaagcc aatggttttt acgaaaaaac
    21961 attggaactg tatcgtttgc taaacgaaaa ggataaaact ttttcttttg aaagagaaat
    22021 gcgtattggc gaaatcaaac aaaaacttct gtaacgagaa attgtaaaaa ttaattgcat
    22081 gactatctac aaaaacaaaa gacgacccga aaaagaaatg atttttggca ttagaccggt
    22141 actggaggcc atacagtcgg ggaaagacat tgacaaaatt ttgttgcgac gcgacatgag
    22201 caatgaaatt tcacgtgaac ttttcgccgc taccaaagga cttcatattc cggtgcaatt
    22261 cgttccattg gaaaaattaa ataaatttac cgacaaaaat catcagggtg ttattgcttt
    22321 tacttcgccc gtaacctatc agcgcatcga agatattatt cctgccgtgt acgaagtcgg
    22381 gaaaatgcct tttatagtag ttttggacgg aataaccgat gtacgcaact ttggtgctat
    22441 cgcgcgaaca tgtgagtgcg ccggcgtgga cgctattgtg attcctgcca aaggtggagt
    22501 ttccattact tccgacgctg taaaaacttc tgccggtgct ttgctttcga ttccggtatg
    22561 tcgcgaaaac aatctgtacg aaacacttca attccttgct gattcgggta ttaaattgat
    22621 agctgccacc gaaaagggag aaaaaaatta tacggcaatt gattttaacg aacccactgc
    22681 catcgtttta ggttcggaag atgaaggcat cagtcccgaa ttattgcaac tttgcaacga
    22741 aaaagttcaa attccgattt tcgggaacat ttcatcactc aacgtttcgg ttgcggcagg
    22801 tattttgatt tacgaagtca tcagacaacg agagcaaaaa aaccaataaa aatttttcca
    22861 gctgttaaca aaatatttgc ttcaaaaagc ttcatttttg catggaaatt ttgtacgaag
    22921 acaatcacat tattgccgta aataagaccg tttcagaaat tgttcaggga gacaagaccg
    22981 gagatgaacc tctatcggaa acgctgaaaa aatatctgaa aaacaaatac aacaaaccgg
    23041 gaaacgtttt tttgggagta actcaccgcc tcgaccgacc ggttagcgga gtcgtattgt
    23101 ttgcaaaaac agataaagca ttgtccagac tcaatgaaat gtttcaaaat aaagaaataa
    23161 aaaaaacata ctgggcaatt gttcagcaaa aaccgcccaa aatggtcgat acgctggaac
    23221 atttcttggt gagaaatgaa gctaagaaca aaaccactgc ttttgatcgg ccggtagata
    23281 atgcaaaaaa agccattctt cactacaagg tgttgacaag ttccgactat tattatttgc
    23341 ttgaaattca acttgaaacc gggcgacatc atcaaattcg ctgtcagttg gcaaaaatcg
    23401 gctgtcctat tcgtggcgat ttgaaatacg gttttccgcg tcccaattcc aatggaggca
    23461 tcagtttgca cgcccgaaaa attgaattcg ttcatcccgt ctcgaaaaat tggataaaaa
    23521 ttgttgctcc tgcgccccaa gacgacaaac tttggcaatt ttttgaagaa tcgcaaaaag
    23581 aaaaagaata atttttacaa ttcatcccaa caaacatgaa ctttcagcga aaacccgatt
    23641 ggataaggac caaattacat agcgaagcaa ctttttcgga agtgcaacaa ataatcgaaa
    23701 aaaacggatt gcataccatc tgcaccagcg gacgctgccc caatcaaagt gaatgctgga
    23761 ataaaggtac ggctacgctc atgattttgg gagaaatatg cacacgtgcc tgccgtttct
    23821 gcaacaccct caccggtcaa ccattgcctc ctgatactac agaacctgaa cgaattgctc
    23881 aaagcatacg atcattgcat ttgcgtcatg ccgtcatcac ttcggtggac agagacgatt
    23941 tgcccgataa aggtgcatcc cattgggcaa aaaccattcg tgcggtgaaa attgtcaatc
    24001 cggctatcac cctcgaaacg ctgattcccg attttgatgg cgaaacaacg ttgctcgatt
    24061 tggttattgc cgaaaaaccg gatattattt cacacaatct cgaaactgtc agtcgattga
    24121 cgcctaaaat tcgttccaaa gcaaaatacc aaacaagcct gaaagtgctg caatatgttt
    24181 cttcctgcgg aataacggcc aaaacaggaa tcatggttgg tttgggagaa accgaagacg
    24241 aaatattttc attgatggat gatgcattga aagcgggttg ttccgttctg accattggac
    24301 aatatttgca accgtcgcga aaaaatattc ccgttcatga atacatatct ccaaaaaaat
    24361 ttgaagaata tcggcaaatc ggtttggcaa aaggattccg acatgttgaa agtggacctt
    24421 tcgtgcgatc ttcctatcac gccgaaaaac acgttttgta aaaacgcttt gccataaaga
    24481 aatttttccg gttattcatt aaatgtaatg agggtaccat tgtcctgcag tatatttttg
    24541 gatttcatct tgatcataaa atattggaaa tcagttaatt tatatgaaaa attttacttt
    24601 tttataaatc aacaagtatg tttttctcgc aagatatcag ttcgtcagac caaatagaaa
    24661 tcatttttta ttcaaaactt aaataaggtt cgattttttt caattgttct tcagtaaatt
    24721 ccgacaggtt tttcagctcg ccgaattgat gcagtttgcc tttctttcgc cttaactcgt
    24781 aaatggcttt tgcctgataa aagttgatat agggatgttt gttcaaacgg tcgacagaag
    24841 aagtattgac ttttattttt tctatcaaat tcggattgac ggtgcagaaa ggtttgattt
    24901 tttccagatt ttccggtgtc attccgtaaa tttccgaaag ttgatcgaca gaaaaaaatc
    24961 cacccgtatt ttgtcgaaat cgaacaatgc tttttgcata atatttaccg attcctttca
    25021 cttgcatcaa aagggtggtg tcggcagaat ttaactctac caatgaagtc ggattttctt
    25081 tgacagcctt ttcgccgaga ttgacgggcg ggttagaaaa tttttcatct atttgaatat
    25141 aaggaagtaa ttcattgaat ttctcagaag gaataccata aattttcgag aaatcttctg
    25201 cgtttttgaa actgccgcct ttgcttcggt acttcaagat gttcgaagcc acataagatt
    25261 ttaatccaag ttgaacgaaa gtagcagaat cagcagtatt aggatcaaaa ttaaaaagtt
    25321 cataatggtt ttcctttttt gaaaaatctt ttcgatagtt caacctgtaa gctgcatacc
    25381 tttcttcaaa ttgacgttga cgttcagctt gttgaatgct gtccagcgaa agcagcgatt
    25441 ttttgaaatt ttccgcctca gcaacaaaat tcgtatcgaa agcattggaa ttgctcggta
    25501 tgagataggg gagagcaaaa cgaacaactg ataccacaaa tatcaacacc agtaaaacaa
    25561 taatgcccac ttgttgtcct ttggaaaaat agaaaaaatc tttccacata gtgctgaatt
    25621 tgagtaaatt ttattagaaa ccgttgcaaa tttaaaaaaa tttttattaa cctttcaaat
    25681 tttcagcaaa ctgaatgtct ttttctttct tcctattcca aaacggcggc gatatatttc
    25741 attttctgat ttgtgaagcc gtcattttcc gaaagtgctt ttcaacaagt gaatttttgt
    25801 aaattttccc aaaggcatct tttttttatg aattgtatta tctttgtgga tgcttaacga
    25861 aatgggagtt ttctgaaaaa taaggtaatt tcatcagaat ataatttatt ttttaaagtt
    25921 ctgatggaac ccacatgtct gaatattata ttcattcgtg tttgtgtatg taatacatgt
    25981 tggtttctta ttccttaata caaattatga caattttaaa taaagcatat gaaaaacgaa
    26041 ttgctaaaga aaattgaaga cagaaaaatt gttgtcgggg tagtcggttt gggttatgta
    26101 ggtttacctt tggcagtgga aaaagccaaa gcaggtttta aaaccattgg ctttgatgtt
    26161 cagccgcaga aagttgaaat ggtaaatgcc gggcataatt atattggcga cgtagtggac
    26221 agcgatttgg atgaactggt aaaaaaagga atgctttcgg ctactaccga tttcagcttt
    26281 gtaaaagacg tcgattttat tgctatatgt gttcctacac cgctcgatga acatcaacag
    26341 cccgacatca gttatgtaaa aagctctgcc gaagccatag gcaagtttct aaaaaaagga
    26401 actatcgttg tgttggaatc tacaacttat ccgggaacaa ctgaagaatt aataaaacca
    26461 attcttgaag aggagtcggg attgaaatgc ggtatcgatt tttatttggg tttttctccc
    26521 gaaagagtag atcccggaaa tttgatttat aaaaccaaaa atacgcccaa agtggttggg
    26581 gctattgggg aagatgcatt ggaagtgatt gctgccatgt acagggctgt gttggaaggt
    26641 gatgtattca ccgtttcgag ccctgctgtg gccgaaatgg aaaagatact cgaaaacacg
    26701 tatcgaaacg taaatatcgg cttgataaac gaactcgcca tgctgtgtca taaaatgaat
    26761 atcaatatat gggaagtgat tgaagcagcc aaaaccaaac cttatggttt tcagcctttt
    26821 tatcccggcc cgggtttggg aggacactgc attccgctcg atccttatta tctttcgtgg
    26881 aaagctcgtg agtacggatt tcatacttcc atgattgaat cttctatgat gatcaatgac
    26941 cgcatgcccg aatattgtgt agaacgagcc agtaaaattc tcaatcgctc taaaaaagca
    27001 ttgaatggtt ccaatattct ggttttgggt attgcctaca aacaggatat tgacgattat
    27061 cgagaaagtc cggcactgaa tgttatcgat ttgttgaaat cggaaggagt gaatgtttgt
    27121 tattttgacc cctacattcc tgaatatttg cgtcatggta aagttttcaa aggagaaaaa
    27181 gaacttactt cggcattgct ggccaaaatg gatttggtta ttgttactgc tgctcattca
    27241 aatgtcgatt acgattttgt tcagcaacac gccaaatatg ttttcgatac caagaatgcc
    27301 atgaaaaatg tcaaaaacag aacgaatata gaaatactgt aacattgaaa tgaacaaaaa
    27361 aatatgtgtt gttggtgggg gcaaatgggg acaaaaccat atcaaaacgc tttatcaatt
    27421 gggcaatttg ggcgctatcg ttgaagccaa tgcagaacgt ttaaacgaat tgctgaaact
    27481 ttatcccgta gaaggattta cagaattgga acaggcattg gaacaagatt ttgacggatt
    27541 taccgttgct gttcctgcgc cactgcatta cgaagtggcc aaaaagatta ttgaaagtgg
    27601 aaagtcagta ttggtcgaaa aacccatgac tttgtcagcc gagacttcca aagaacttgt
    27661 tgaactggct caaaggaaag gcgttcaatt gatggtgggt catgtattgc ttttccatcc
    27721 ggctattcga aaaattaagg agataattga cagtggagaa ttgggaaatc tttattatct
    27781 ttattccaac aggttgaatc tcggcacggt tcgtacggaa gaaaacgtgt tctggtcgtt
    27841 tgccccgcac gatatttcgg ttttggatta ttttgtgggt catccggctg aaaagattgt
    27901 ttcaaaaggt tcaaaatttc ttcaggaaga agtttacgac gttaccatta ctcaactgga
    27961 ttatccgaat aatgttcacg ctcatatttt tgtatcgtgg cttcatccgt ttaaagaaca
    28021 gcggctggtg gtcattggta gcaaaggaat ggtctccttt gatgatgcta cggctgaaaa
    28081 agagattaaa ttttacgaca aacgaattga ttttgaaaag ggaattccag tgaaagttga
    28141 aaatcctacc agaatcattc cttatgaaca aaaacaacca ctctccgaag aattgaaata
    28201 ttttatcgaa catctggata aaaaaataga aatagccgat ggacaatcgg gtttggaagt
    28261 ggtaaaagtg ctggaagaag cagagaaaaa gatacaggta gggtaggaga tagtagtttt
    28321 gagttatgag tttcgagttt agagtaaaga gtcaagagtc aagcgttgca agatttggat
    28381 aagatttttt attaatgatt tgaaaatcag cgagtaaata ttcagtagag acgtaacatg
    28441 ctacgtttaa agagttaata ttttggagtt ttgtgtaata agtgaggagt aaagagttac
    28501 aagttttgag tttaaagttc atagtttaaa gttattatta gtattagttg tgagttataa
    28561 taaattaatt tgttattgct cctgcatttt attaaaactc aacactctgt tttctacact
    28621 caatactcat tactcaatat tgaaagactt aaattattga ataaaaacaa aatactttat
    28681 ggaaaaagaa tattttgtac acgaaagcag ttatatcgac gaacaggtaa aaattggcaa
    28741 gggaaccaaa atctggcatt tctgccatgt gcaaaagggt gcagaaattg gtgaaaattg
    28801 ttcgttgggg cagaatgtaa acatagccaa caatgtgaaa atcggtaaca atgtccggat
    28861 tcaaaacaat gtttccgttt acgaaggtgt ggaactggaa gataatgttt tttgcgggcc
    28921 ttcctgtgtg tttaccaatg taaaaacacc tcgcgctcat tatcctgttc atggcattta
    28981 cgacaaaacg ttgattaaaa atggcgcttc actgggagca aacagtacgg tcgtttgcgg
    29041 acatacagtt ggtcggtcgg ctatggtagc tgccggagcc gtcgttacca aaaatgtgaa
    29101 ggattatgcc cttgttgccg gcattcccgc ccgacagatt ggctgggtgt gcgaatgtgg
    29161 cgaacgattg gaggaagatt tggtttgtaa aaaatgcggg aaaaagtatc aggaaacaga
    29221 aaacggattg aaagaaattt atttataagt tgttgatatg aaacccacat gtattgcata
    29281 cacaaacacg aatgaatata atattcagac aggtgggttc catcagacct ttaaaaaata
    29341 aattatattc tgatgaaatt taattttttt taaagcgaat gatcagtcct aattgagtta
    29401 agttttcatt aaatgaattg aaaacctatt taaaaataaa aggaaataag atctttacaa
    29461 atgagtgtta aaagaaaaat atttttaata atctttttgt ttttaattgc aacgggcata
    29521 ttttttctta ttaaaaatca atcttcgtcg aaatatattt ttaatgaagg aaaaattcat
    29581 ggtacctatt atcatgccat ctatatgcat cctcaaggaa ttgatttaca ggaaaaaatt
    29641 gaagagcgca tgaaaaaaat agatatgtct ctttcaactt ttgaaccaca atctgtaatt
    29701 tcccgaatca acagaaatga cgattctgtc ttgacagatg atgattttga aactgtattc
    29761 aaaacagcac aaaaaatttc tgaaattact tatggcgctt tcgacattac tgttgcaccg
    29821 cttgttaatg cttggggttt tggttttgat agccacgata gaattctgca accaaatctt
    29881 gataccatca tgccttttat tggttatcaa aaaatcaggc ttgagaacca taaattgata
    29941 aaggattatc ctaaaatcat gctcgatgca agtgctattg caaaaggtta ttccacagat
    30001 attgcagcaa atgttctcga agagaatggt tgtaaaaatt atttggtcga aataggaggt
    30061 gaaattgtgt gtaaaggaaa aaacacaaaa ggagaaaaat ggaaaattgg gattgataag
    30121 ccaatcgatg atgaaaccaa tacactgaat gaaattcaaa cggtgatttc aatcaccgac
    30181 aaggcaatgg caacttcagg aaattataga caattttata taaaggatgg aaaaaagtac
    30241 tctcatacta ttgatccacg aacaggctat ccggtcaata aaaatatatt gagcgcaact
    30301 gttatagctc caaattgcat tacagccgat gctttagcaa cagcttttat ggtcttggga
    30361 gtcgatagca gtcttatgat ttgtagaaca ttaccggaag tagattgcta tttgatatat
    30421 aacgaccaaa aaggtagtaa taaggtaata tacagcgagg gatttgaaaa ctatttttca
    30481 aaataataac caaaggagtt gatcattccc gcaagctgat caaaatattt ttttattctc
    30541 agtaaaataa ttacttttgc aatatatttg cttgttatga aaaaatattt tttggttttt
    30601 tctattctga tatttctttt ttcttcatgt agcgagtacc aaaaattgct caaaagcacc
    30661 gatatagaat taaaatacaa taaggctatc gaatatttca acaacggtga ttttgctcgt
    30721 gctacaactt tattcgaaga tatttcatct tattatcgtg gaactgaccg atcggaagat
    30781 atattgaatt ttttaggtca gtcgtactta ggacagaaag attattatac cgcttccaat
    30841 tattatcaaa cctacttgaa atcgtatcca aaaggacgtt acgcagaaaa tgcaaaattt
    30901 ctggttggat attgctatta taaagattcg cctgatccca ggctcgacca aactgctacc
    30961 tatcaagcta tagcttcatt tcaagaattt ttggatttat atcccgaaag taataaagta
    31021 ccccaagtaa acaaattgct cgatgaatta catgataaat tggcttacaa atattatatg
    31081 aatgcccgat tgtattataa tcttggaaat tacatgggaa acaattattt gtcggcagta
    31141 ataaccgctc aaaatgcgtt gaaaaaatat ccttccagtt catacaggga cgatctttct
    31201 tttttaattt tacaggcaaa atttgctcaa gctcaacaaa gtattcttgc caaaaaggaa
    31261 gaacgttatc aggataccat tgatgaatac tatagctaca tcaacgaatt tcctaacggc
    31321 aaatttaaaa aacaggccaa taacatattt aatgattgca aaaaggctat caacttaaaa
    31381 taaaattaga atatttatgg attttaaaaa aatgaatgtc ccttcaacaa ccatcacacg
    31441 agatataaac cttataggcg aaaaggtggg gaatatttat gaagcagtag taattattag
    31501 caagcgtgcc aaccaaatta gtttggaaat aaaatcggaa ttagacaaaa aacttcaaga
    31561 gttttcagca atgggtgata atattgaaga agtatttgaa aatcgtgaac aaattgaaat
    31621 ttctcgttat tacgaaaaat tacccaagcc aaccttgatt gccattgaag aatttttgga
    31681 agataaaatt tattatcgca atccgataaa agaaaaatta aaatagggtt tcagccttaa
    31741 actactgttt ttgaagaata tttttcaaaa aaattgattt aaatttattt cgatattttc
    31801 ctcatttgtt gtttgttatt aatagcttca cgctaatagc aaacaacaaa cgaggtcatt
    31861 gagttgaatt tagaagattt tcgagatgca ttcaataagt ttattaaatg tgagaagcag
    31921 gtgaatgatt tgtacttaac attttctcaa tagcttacct tactatcatc aatcaatttg
    31981 attttcactt attaagtttc tctccccacc aaacttcgct ttccatttca gttaaaaaaa
    32041 taatattaaa aacaaaaaag agtaaaattt tgagtgggtt gaatgaccca tacgcctgga
    32101 atttgatttc tgaaaaaaaa cacgtatttt tgggcgaaca aaaaacaatt aaaatttatt
    32161 tcgaaacatg gctaaagaag ttgctcaaaa ttcgggaatc atttataatt cgttgaccaa
    32221 cggaagtaaa attgttggaa caatcacggc cgaaaatgat ttcagaattg acggagaagt
    32281 ggaaggcgaa attttttgta acggaaaagt agtgatcgga caaaagggtt acctgaaagg
    32341 atcaatttcg tgcaacaatg ctgaaattat tggaaccgta gaaggtgaaa tatcggtaca
    32401 agacacattg tctttacgtc aaactgcaaa agttacaggc aacgtaaaaa ttaatgtgct
    32461 aatcatagaa cccggcgcta ttttcaatgg acaatgttgc atgaatgaaa cagattcgat
    32521 tcaggaaagc gaataaacaa ctcgctttat tttcctgaaa ccaatcgaaa ttcaacaatt
    32581 caatttttac catcaaataa aatttgggaa tggaaaatat cacctataaa gctttagttg
    32641 ttgaagagga tggtaatcga tactcaagaa aaattaaaga actgacaacc gaacatttgt
    32701 tttcgtatgg tgatgttctt attcgggttt attattcttc gctcaattac aaggatgcac
    32761 tgtctgccac cggcaacaaa ggcgtaacaa aaaactatcc tcacacgccg ggaattgatg
    32821 cggcaggtgt agttatagct tcaaacaacg aaaaattcca atcgggcgaa aaagttattg
    32881 ttaacggtta tgacttgggc atgaatacgc ccggaggctt tggagaatac atttgcgtcc
    32941 cttccgactg gatcgtaaag cttcctgaag gtttgacact gaaagaatcc atgataattg
    33001 ggacggcagg atttacggca gggatttcac tgactcgact cactgagctg gtttctcccg
    33061 atatgggtga aattattgtt tcgggtggaa caggtggtgt tggttccatg tctctttcca
    33121 tgcttgccaa acttggctat caaaccgttg ccattacagg taaaataacc gaaaaaagtt
    33181 tttttgaaac cattggttgt cataagattc ttccgcgcag cgaatttgag tccttgccgc
    33241 aaaaaccgat tctatcagct aaatatgccg gtggcattga tactgtaggt ggaggaattc
    33301 tggaaaatat catcaaacag atacagccgc tcggtgccgt tactacttgt ggcagcattc
    33361 attccaccga attgaatatt tccgtttttc cctttatttt acgtggcatt tcgctcatag
    33421 gcgtttcggc acaaaattat ccaatgaact tacgggaaaa attatggaaa aaattggctg
    33481 ccgactggaa gcccgaacag cttttaaatc tttacaccga aatttctctt ccccaacttt
    33541 cagaagctat agataacatg ttgcaaggca aactgaaagg tagaattctt gtcaacctga
    33601 ctgcctgaga aaatttacga ataaaaaagt cgtctaaagt caaataacga tagacgaccg
    33661 gttaacataa aaaatttgct gcaaaattac ttctttttac gctggtaaac tgaaaatgtg
    33721 taaggataag ggtttttttc atcggccgga tgatcttctc tgctgatttc ttcccattct
    33781 tcaaaattaa ttttcgggaa ataagcatct gcctgaaaat ctccatgtat ccacgtaata
    33841 tacattttat ctgccaaatc gatggtttgc ttaaaaatgg atactccgcc aataataaaa
    33901 acctcgtctt ctttttcgca caattcgaga gcttcttcaa tggagttggc ttcgaaatag
    33961 ccttcacaaa ttccatcgga caacaccgaa gttaacacaa tattttttcg gtttggcaaa
    34021 ggcccattgg gcaaattttc aaaagtgtgt tttcccatga taactgcatg accggtagta
    34081 agttttttga aatacttcaa atcggccggc aaatgccaag gcagtttatt caaattgccg
    34141 atggcataat tatcggcaac agcagcaata attgaaattt taggcatgat aggtaatttt
    34201 taatccatat tgataattct cacaaaaaca tgcaaactta caaagttata aaaaacttat
    34261 tgtaaaacct aaactatttt ttcagaaaat aatttcaaac agctacttcc cctttaatgt
    34321 gaggataagg gtcataatta attagctgaa agtcggaata ctgaaaagaa aaaatatctt
    34381 ttacctccgg gttgatcaac atggttggca aaggacgtgg agttcgcgta agctgcaatt
    34441 ttacctgctc cagatgattg gtgtaaatat gggcatctcc aagcgtatga acaaaatccc
    34501 cagctttcag tcctgtaact tgggccatca tcatcaggag cagagcataa ctggcaatat
    34561 taaagggaac acccaaaaaa atatcggcac tccgctgata aagttgaagg ctcaatttgc
    34621 cgtcggccac ataaaactga aagaaagcat gacacggagg caacttcatt tttggcaaat
    34681 cggctacatt ccatgcactg acaatgattc gccgtgaatt gggattgttt ttgatagcct
    34741 ccaccgcttg ggcaatctga tcaatataac cgccttcata atcgggccac gagcgccatt
    34801 gataaccgta aattgggccc aaatcaccat gttcgtctgc ccattcgttc caaatactta
    34861 cgccgttttc ctgcaaaaac ttaacatttg tatcgcctct cagaaaccag agcaattcat
    34921 aaataatcga ttttaaatgc aattttttgg tagtcaaaca agggaaccct tcgtccaacg
    34981 aaaaacgcat ttgatgacca aaaacactta aagtccccac tcctgtacgg tcttctttat
    35041 gaactccttc tgtaagaaca cgattcaaca aatccaaata ttgtttcatc ccaaaaaatt
    35101 ttaataagca acaaattcaa gttaataatg caacttttta cgatttgtaa taattttcag
    35161 cgctaaagat caatacaaat tataagtcgt ttttaaaaca cggaattcgg tacgacgatt
    35221 aatttgattg gcaatttcct gttgttcggg ggtgagagtt aatatataac tttccgtcaa
    35281 agtttctccc actttcagaa atggatattt ctgggccagc gattcatcta ccactatcgg
    35341 tttttcctct ccatacccca ccgctgtcaa ccgctccttg tctattccgg cttttatcaa
    35401 ataattcacc accgactgcg cccgcttttc ggaaagattc ttattgaaag cattgtcgcc
    35461 aatgtagtcg gtgtgcgaac tcaattcaat ggtaatattc ggattgtctt tcagcaattt
    35521 aaccagttcc tgcaaagatt tttccgaagc aggcgtcaaa tcccattttc caaattcata
    35581 aaaaatattt tcaattttca ccggtttact gatagatgaa agcttgaaat ttacattgaa
    35641 agttttgctg tcggtaagtc cttgtgttga aagttcattt ttttgattca gaaaaccgcg
    35701 agcggttgcc agcatcacgt aatcgacatc tttttcgagt ttaattcgaa aagtaccatc
    35761 cttttttgtt tgaatgcgag cattcatccc attgctgcca acaatacgaa ttgtagcatc
    35821 agaaatagtt tgtccctgat catcaaatac ttttccttca accaaataaa tcaattcggg
    35881 caaatgaaaa ctccacaaag catcgtaacc tctcatttcg ttgcgatttg aagaaaaata
    35941 accgctttcg ctttcaccgg caaacgtcat cccaaaatca tctgcctttg aattaaccgg
    36001 tgctcccata ttttcaacct tccatcctcc atttttttgt ggaacagctt taaaaatatc
    36061 caatcctcca aatcctggat gtccattcga tgaaaaatac aaagttccat cggctcgaac
    36121 aacgggaaac atttcatcgc cgggtgtatt gatttccgga cctaaatttt caatgtattt
    36181 gcagtcggtt ccttccaatg tggctctata aatatctttt cctcccaagc catctttttt
    36241 gtcggaaaca aaataaagtg tttgtccatc gggtgcaatg gcaggatggg caaccgaaat
    36301 cgaactatcg gaaaaaattt tcaacatttg aggtggactc caggttgcgc ctgctcggga
    36361 agatacataa attttcgtac ccatatctac caaatcggat tgctgagccc gtgtgaaata
    36421 aatggttttt ccgttagcgg caaaggaaca aacgccatcg tcggtttcgg gtgtatttat
    36481 ttctccttcc aacaattcgg gttttcccca ctttccagtt gcattttttc gcgccagaaa
    36541 caggttgttt ttgggtaaac cggtgatggg atcggctttg gctgtggctt ttttatcggt
    36601 agaacgagta gaagtaaaaa ccagttcgtc agcagctgta tttacaaatg cgggactgaa
    36661 agtggaagct cttcgaacgt taaactgatt atcatatttt acaacataac gggttgcatt
    36721 tttctgccat tcctccaaca tttgaatggc acgaatacca ttttttgcaa taatgcagga
    36781 agaatctttt tgcaaataca gctgataatt gcgtaaagct tcgccatatt ttccatttcg
    36841 atgcaatact tgcgcatacc gaagaaaaac ggtgctgtcg gcaaaattgt ttcgaatagc
    36901 attcagataa gcctgttcag cccgttgatt gttgatgagc cggtaacact caccctgtcg
    36961 aaaagcaaca tatccgcgca atgatttatc cttattggga atccgtccat acgaactttt
    37021 atacaaatcg cccgcagcat aatattcccc caactcaaat cgcgaatcgg ctttttttat
    37081 acgtgaactg acgccacaac tttgcagaaa taaaagaagc aaacaaagcg aaataattcg
    37141 atagagaata aaagacggtt tttttataaa aaattgcata aatatttgtt aatcggcaac
    37201 tcgtaaaaat tttgtaatgt cgtaaaatta aaaaatttaa taccggtatt gttttattaa
    37261 tttagctcag ctcatgaatg accagcccgg aacgcaattt tggttcaaac catgtggttt
    37321 ttggcggcat aatgtttccc gtgtcggcaa tggcaataag ttgctgcatc gatacgggat
    37381 aaagtgccaa agcgacccgc atttttccgc tatcaaccaa ttttttcaac tcacccaaac
    37441 cacgaattcc tcccacaaaa tcaatccgtt tatcagtcct caaatcgctt attccaagaa
    37501 ttttatccaa aatatatttt gaagagatag taacatcaag accttcaatg ggatcattgt
    37561 cgttgtaggt tccttcacga gctgtcagcg aataccagtt tcccgacaaa tataacgaaa
    37621 aattatgcaa tttatttggg cgataaattt ctgtacccat tttttcgaca ataaaatatt
    37681 gggaaagctt ttgtagaaac tcttcatcgt caagctgatt caaatctttt accaccctgt
    37741 tgtaatccat aatttgcaac tggttgtcgg gaaaacaaac agccataaaa taattgtatt
    37801 cttcctttcc ggtatgatgg ggattttgtc gtcttttctc atcgcccacc cgaacagccg
    37861 cagctgaacg atggtgccca tcggcaatat aaagagaagg aattttttga aaaatttccg
    37921 agatgagatg aataatttct ttatcgtcaa ttttccagaa atggtgtcca aagccatcgg
    37981 gcgcaacaaa attataaact ggctctgatt taacaatttc cgaaacgatt ccgttcaatt
    38041 cttcatttga aggatatgca agaaataccg gttcaacatt ggcattggta acccgaatat
    38101 gtttcatgcg gtcctcttcc ttgtcgctgc gtgtcaattc atgttttttg atgtgcccgt
    38161 tcaagtaatc gtcaatatgg gcgcaaacaa ctattccgta ttgagtacgg ccattcatgg
    38221 tttgagcgta aatgtaaaaa tgttcttctt catcctgaac caaccaacca ttttcacgaa
    38281 atttcttgaa attttctgct gcttttgcat atactctttc atcatgttca tccgttcctt
    38341 caggaaaatc gatttccggt ttaataatgt gatacagcga catcggattg ccttttgcct
    38401 cttctcttgc ttcttccgaa ttcaatacat cataaggtcg agaagccact ttttctacaa
    38461 tttcgcgtgg aggacggatt cctctgaaag gttttacaat agccataata tattactttt
    38521 ttaagtttat tatttcatcg caaaagtaca caaaaaaatt tattttataa ttcctgtcca
    38581 cgaccaatta tcatccaatc aatgtattga aaaaagaaat agataattta aatctatata
    38641 tttgcatact ttttgacact tccgtaattt tgtatgatca gaccaagagt tccatccatt
    38701 tacaataacc aactattctc taaaaaatat gcgcgtactc gtttcaaccc gtttaccgga
    38761 agaaggtttt tcggaattaa tgaaaaaatt tgaagttgtt tttcctgaaa aagaaatatt
    38821 ttcaaaagaa gaagtcatac aaattattcc ggagtttgat gcgtttattc caacttttca
    38881 atttaaggtt gatcgagacg tcattggcgc cggcgctcaa ggaaaactga aaattattgc
    38941 caattatggg gtcggatacg acaatattga tgttgatttt gccaccaaat gcggcattgt
    39001 tgtaaccaat acgcccgatc ctgtagtgga accaacagcc gaaatggcga ttgccttaat
    39061 gctgtcagta gcacgacgcg tgagcgaatg cgatagaaaa ctgagaacag ataacagtat
    39121 caaatgggga gtaatggaaa atttgggtat ctcactttac ggtaaaacac tgggaattgt
    39181 cgggctggga cgcatcggga aagctgtggc tcgaagagct gctgcattgg gaatgaaaat
    39241 tatatatttc aaccgaaatc ctttggacaa aaaaacggaa acaaaactta aagctcgcta
    39301 tgcaccactc ggacaactgc tttgtgaaag cgatgtcatc agtttacatg tacctttgac
    39361 cgaaagcact ttccatctga tcaatgaaaa cacacttcgt ttgatgaaac ctacggccat
    39421 tttaatcaac acctcacgtg gcccggttat tgacgaatcg gcattgacgg aagctttaac
    39481 gaaccataaa atttttggtg ccggcctcga tgtatttgaa catgaaccta caattccgga
    39541 aaatttgaaa aaactcgata acgtcgttct ttcgcctcac aacggaacag ctaccattga
    39601 ggatcgaaac gcaatgagca gatttgcctc acaaaatatt ctgcgttttt ttgagggcaa
    39661 caaaaatatt tccagggtca attaaacgaa tgacacaaat caaagtcaaa caaaatgaag
    39721 aaaaagtagc ccgaccaact tgaagtggag aacgataaaa atataaaagg cgaagaaaaa
    39781 ctgaaagaac agctgcaaga cccgaaaaga aaagctgcag ctttctcgca actggtacgc
    39841 atgtatcagg aacgtttgta ttggcatatc cgaaaaatgg tgctgtcaca cgacgatgcc
    39901 aacgatattc tgcaaaatac ttttatgaaa gcttggaacg gaatagacaa cttcagaggc
    39961 gactcacttg tttctacctg gttttaccgg attgccacca acgaaacact gacttttctg
    40021 gccaacaaaa ggatgaaaaa catcaattcg ctcgacgaag tggaagatgt gttgctgcaa
    40081 cagttgcaag ccgaccaata cttcaacggt aacgaagctc aattaaaatt acagcaagcc
    40141 attttaactt tgccggaaaa acaaagactg gttttcaata tgagatattt tgatgatctc
    40201 tcttacgaac aaatggaaaa catattggga acaactgtcg gagcattgaa agcatcctat
    40261 catttagcag taaaaaaaat tgaaaaattt ttactcgaca gcaatattga aatttagcaa
    40321 ctaatataac aatttgattt ttatcgattt ataataaatg atgtactaat taatattttc
    40381 aaattaaacc taaaccattt tttacagtct aacaaatagt tatgaaaaaa gaaaaaaaca
    40441 tattgaaaaa aattggaaac gacttacctt tcagcgttcc tgaaaattat ttcgagcaat
    40501 ttgccaatga aattgatcgt caaattggct atgcaaaaga aatggaagtt tccaaatcgc
    40561 aaaaaagaag ttttgccgtt atttcgaagt catggctcta tatggctgct atgtttgtgg
    40621 gaatattttt gctcggcaat gttttttatt tggctttcaa aaactccgaa cgccaaaaac
    40681 aggaaaacta cgaaatgtat ttgctctcgc aagtggacga accaaccatg atgtattact
    40741 atatgaatga tgcttatgaa gcagaaaatg gtactgaaac ctttcaaaac acttcaaatt
    40801 aatttcaaat gaaaaaattc aaatatattt tgatgctaat ggtgtggata actgcaaact
    40861 cgctttcagc agaagaactc gtcaacacca atatccagaa aatgcacgaa aaaaaatggg
    40921 cttacatagt tgaacaggca aaactgaccc ctgatgaagc ctcaaaagtg aaacctgtat
    40981 ttatgaatta cgaaaatgcc gtatgggaat tgcatgaaaa aaacagagaa acattgaata
    41041 attttctgaa ggaaaataaa acgcagccaa actatgaact gctgaatgat gaatatgtta
    41101 atttgcaaat aaagcaggca cagttactca aaaattatca tctaaaatta aagaaagccc
    41161 tttcacccga aaaattattt cattattatc aagcggagcg aacttttaaa cgcaaattgc
    41221 ttcataatat gcctcagggg aggaaaatgc aaaaaaaccg tccctaaggt tttttattaa
    41281 gctacttttc tgcggaaagc tttggagtga aggaaaaaag ttctatcttt gcagaccaaa
    41341 acaaggtact ttggccgaga ggttaggcac cggtctgcaa aaccgcatac ggcggttcga
    41401 atccgccaag tacctcattt ttagcaagct ctctttcgtt tttgcgaaaa aagagcttgt
    41461 ttctttttta tggacagtaa taattccatt tttcacaata aggcggccag tttattttcg
    41521 agtttcagca aatctttggt gaaatttcta attccttcgg ccaatttctc ggtagccata
    41581 gcgtcttcat tcatcatcca gcgaaaacta atttcattaa ttttcaaagg ttgaatgtcg
    41641 gcagtttttg ctttttccgg atctaatttc ctttccagca caccttccgt ttgctcgagt
    41701 tctttgagca aagccggcga aatggtcaac aaatcgcatc ctgcgagctg aatgatttcg
    41761 cccatatttc tgaaactggc gcccatcact tgtgtggcat aaccaaactt tttaaaataa
    41821 ttgtatatgg ctgtaacaga ttttactcca ggatcttctt caggtggaat atcggaaact
    41881 ccacgatctt ttttgtacca atccaggatt cttccaacaa agggcgaaat caacgttact
    41941 ccagcttcag cgcaagcaac ggcctgcgtc aatgaaaaca acaaagtcaa attgcagtga
    42001 atgccttctt tttccagtat ttcggcagcc cgaattcctt cccaagtaga agcaatttta
    42061 ataagaattc gttctcttga aattccttcc gattcatata gagcaattaa ttcgcgggct
    42121 ttggctattg ttccctttac gtcgaacgat aaacgtgcat cgacttcggt tgaaacgcgt
    42181 cccggaacga tttccaaaat tttgataccg aaattaacag ccaatttatc cattgctttt
    42241 ttcaactgtt cttccttatc agaagtcaac gattgggcat agcgaatagc atcttccatt
    42301 aaatattcgt attttggatc ttgagaggca gaaaaaatca atgaaggatt ggttgtagcg
    42361 tccacaggct gataagccat catggatgca aaatcccccg tgtcggcaac cacagtcgta
    42421 aattttttta attgttccag taaattcata agtttaagtt atataaaata aaattttcaa
    42481 tggaataagt taatagataa tttaaatgat ttattgacat tcaattgttt aactgaaaat
    42541 ttatcacatt ttcaaaaaaa tcacctatca aagtttgatg taaattttct ttttgtagag
    42601 tatatgtgca attatccaat tgatgaaaac aaaacataaa gcaaaaatga gtgatcccaa
    42661 ataatttcca aaaacagaag ataaccactc aaaatacacg aaattcttaa tggaaatcca
    42721 attgccttga tacataaata caacattgct tagcaaaata cttaaaatat cggcaactac
    42781 ataaataaac aatggattga ccccaaaaga ttcaaaaaac acactccatt tctttttatt
    42841 gtttacgtcg ataatccaaa ttagcaaaga aaaaagcaag gaagccaagc cacaagttac
    42901 tagaacaaag ctggaagacc aaattttctt gttaacggga aaaccataat ggagaaaaaa
    42961 acctgcaaag aaaagtatag tcccaaaaat tgccaagttt tgaatttgtt catttttcga
    43021 ttttgattca gaaatcagtt ttcctgccca aaaaccaatt aatacatgtg ctatgcaggg
    43081 taatgtgctc aaaattccct ccggatcaaa gggaataact tttccgtcag gtaaagtttc
    43141 ggtatacatg tgtgaagcgc ccagtatagc tctatccaca atcgcaatga tattgttttc
    43201 gctggcaaca taacttccgg tgaccccaat cagaatccag taaattaaca atattgctcc
    43261 cgttaaacag ggaatgtgtt tgtgcggaat caacaaaatg atcaatgtac caaaaaaaga
    43321 taccaaagcc aaccgttgca aaacgcccaa aatacgaaca ttttcaaaat tggtggctgc
    43381 ttgccagatt ctttcaccca atggtatatc tgttccttgc aattcacgta atccgccact
    43441 gaacagtccg agccagccaa tagccaatcc aatcaaaaat agcaatgaag accttctgaa
    43501 taattttaaa aaggtgcgat tagaaaattt aaaatcgaat ttcttgtagg ataaatacat
    43561 ggaaacaccc atgataaaca taaaaaacgg aaaaaccaaa tcggttggag ttaagccgtt
    43621 ccattctgca tgtcgcaagg gtggataaac acttccccaa ttacccggat tattgaccaa
    43681 aatcattccg gcgatggtga tgccgcgcaa tacgtcaagg gaaagcaaac gagcagactt
    43741 gcctgtagtt gataccataa ataatttttt aagcagttaa atttaaggaa aattattgtt
    43801 gcaaatttaa tgaaaatatt ttctttaaat attcatagat taaaaaagat aattcaaaca
    43861 tgaagcatca tgtgaagaaa gaattgcgag ttaattagta gaattttttt aaaataaata
    43921 ataacgaaca taagttcaaa ataatttata tatttgtagt ttgaaaaata aaaaacatgt
    43981 cacgtccccg acaaaagcgt ataataaaat cgcctccgct tatgatgggt tacaagcctt
    44041 ttggcattcc acataaggaa ttggataaaa ttgaactcca ttttgatggg tttgaagcca
    44101 ttcgtttgct cgattatgaa ggaatgacgc aggaagaggc agctggacac atgaatgttt
    44161 cgcgacctac actaacgaga atttacgaat cggccagaaa aaccattgcc caagcattgg
    44221 ttgaaggaaa aatgatagta attaaaggcg gaaatattga gtttgagcat caatggtttc
    44281 gttgtaaata ctgttatcaa ttggtcaaag aaggcgaaac tcatgtacat caaaagtatt
    44341 cggatggaaa cgaactatta cccatagaaa gttagataag taattcattt atttcgtagt
    44401 aaatcaaaaa ataaaattta tgtccgaaag ttttctttct gctgttgttg ctttgttttt
    44461 aggaagtgtt ggcagcgtac tgttggccgg tttaattttg ttgttaaaag agcggcagtt
    44521 gcatagggtt acacacgttt taacttactt ggcaggaggt acattacttg gatcagcttt
    44581 gttgggaatg attcctgaag cttccgacat gatggaaata aaaaaggtgt tatatgtggt
    44641 acttgctgga attatttttt tctttgttct cgaaaaaatt attttatgga gggtatgctc
    44701 cgacgcttca tgcgaaagaa atcttcaaac agcggctcca atggttttat ttggcgatgc
    44761 cttccacaat gccattgatg gggtgattat tgcttcagcc tttttaactt ccaatacttt
    44821 tggttgggtc gtaactttag cggtaatttt tcatgaagta ccacaggaat tgggcgattt
    44881 cgggatactt ttaaaaagcg gaatgaaacg ttccagagca ttgctttaca atgctttatc
    44941 aggagcttcg gctttgatat tcggaatagt tgcgtatttt tcgctcaatt ttgccaagtc
    45001 tttgattcct tatgcattgg cattttctgc tgccggtttt ttgtacattg ctttggctga
    45061 tttgattcct gaaatgcaca gaaaaacgtc attcaaggag tcgatggtgc agattttctt
    45121 tattttgttg ggaattatgc tgatttattt ttttggaaga gattagcaat cactgatttt
    45181 tatatgaaac cctcatgtct tacatataca aacacgaatg aatgtaatat tcagacatgt
    45241 gggtgccatc agacaattaa aaaataaatt atattctgat gaaatattcc aaatccagag
    45301 ggtcgatcac aatggcattt tttgcaggtt aagcagctaa ttcaagagtt aaattttgtt
    45361 tggaaattgt gttatttatt gatttctgga atatacaaca ataacgacaa aaattaggga
    45421 gcagggaaaa atgttttaga gtaaagattt tattttactg ataaccaccc gtatgaatgc
    45481 tacttatgtg tttagtcttt tattggtatt gtttcagatg ccaagaaata aaaatgcaac
    45541 ttttagaaat ttttcgtcaa aacttacatt tttttaattc ctccagtgcc ttattctctt
    45601 ttcgagtcat ttctgctttt aattcgggag taatatcatc ttgtccgcaa agatggagaa
    45661 taccgtgaat gatgactctg tggagctctt cttcaaagtc gacacagaaa tcttcggcat
    45721 tgcttttcac agtttccaaa cttataaaaa tatcgcccga aattgtggaa tccgtactgt
    45781 cgtcgaaagt gatgatatcg gtgtaataat cgtgttgcaa atattgtcga ttgatttcga
    45841 ggatgcgttc gttgttacaa aatatatagg caatttctcc aacttttttt tcgtaattgt
    45901 gggctgtctg ttttatccag aggttgattt tttgtttttt cagtgccggc atcggaatgt
    45961 tttcgctaaa atattgaatc atgacaaaat gaaatataaa taggttttca cataaaagaa
    46021 atttatgaaa ttttcagaca aatatacttg attttgctga agaatgtttt gaaggaactt
    46081 tagttttttc tttttgcatt ttttccaaaa aaaatatcct tttgttcttt tccatagtgg
    46141 gaggaacaaa aggataaaat taattgagca aacaaagaat aaaaaggttt ttcagcgtat
    46201 ttgtaaatta aaaaaattat tgaagtttgt tgagtgcctg ctgaattctt tcaaaagctt
    46261 ttttcagatt atcttcggat gttgcatagg aaaagcgaat acattgcgga gcaccaaagg
    46321 ctacaccgcc aaccgatgca acatgagctg tttcgagtaa aaacatggca aggtcggcag
    46381 aattttcaat aacctttccg ttgaaagatt ttccaagata ggcactgcaa tcgggaaaaa
    46441 catagaaagc accttgtgga acactaactt tcaacccggg tatttggcga accagttcca
    46501 ccaccaaatt tctacgtcgt tcaaaggcag cacgcatggt ttccacgcac gactgatctc
    46561 cggtatacgc ggcttcggcg gctttttgag caacggaaca agctccggaa gtatattgtc
    46621 cctgtaatgt tccgcaggca gaagcaatcc atttcggagc ggctatccaa cctattctcc
    46681 agcctgtcat tgcgtatcct ttggaaacac cgtttataac taccactctg tctcgaattt
    46741 cttcaaattg ggcaatactt tcgtgtttcc caacataatt gatgtgctcg taaatttcat
    46801 cggagagaat gagtatttgt gggtgttttg ccaatacttg agccaatccc tgcaattctt
    46861 ctttggtgta aacgctgcct gtaggattgg aaggagaaca aagaatcagg gctttggttt
    46921 tttccgtaat ggctttttcc aattgttccg gagaaatttt aaaatcattt tcaatgtcgg
    46981 aatagataaa taccgattta ccttcgccca acttgaccat ttcgggatag cttacccaat
    47041 atggagccgg aataatcact tcatcatcct tgttcaaaat tgtcaacagc acattgcaaa
    47101 tggcttgttt tgctccattg gaacaaacaa tctgatcggg ggtaaattcc aagttgtttt
    47161 catttttgag ttttgagcaa atagcttttc tcaattcgag aaaccccggt acaggcgaat
    47221 agaaggagta attgttgtca actgcttgtt ttgcagcagc tttaatgtga tcgggagtgt
    47281 taaaatccgg ttcaccaacg ctgaggttga taacgtcaaa tccctgagcc ttcagttcgt
    47341 tacttttttg agacatggca agcgtttccg aaggtgataa ggctgccaaa cgttctgata
    47401 ctttaaacat tgctttttct aattttttgt agtgattttg atgcaaaaat aatcaaaaaa
    47461 ttacaattta tttgatttta aaggcaattt gttgtaataa ttcttgttta tttttttaat
    47521 tagaattaaa tatctgtttt cctgataaat agataatttt tttatgaatt tttattttct
    47581 ttttttatgg tttggaccca agacccaaca ggtttatggt ttttcgtgaa aaaaattgac
    47641 atgaaaaatt aagaatcagt gattttttca gattcaaaca cacacaaaca cggcaattac
    47701 cttgtatcag ttgagcagta atattatgta atttctttaa gcatgaccag tgcagcctgc
    47761 gtggaacgtt gaataatttg ttcgcgggta tttccaaaat gaaaaagtcg gcttgtcatt
    47821 ttatcttttg agcaggcagc tatccaaact gttccaaccg gtttttccgc cgtaccgcct
    47881 gaaggtccgg caataccgga agtggctatg gctgtgtcgg tgtgcaaaag ctgcaaggct
    47941 cctcttgcca tttgttctac cacctgccgg ctgacagctc catataagtt caaatcttcg
    48001 gaggatactt gcaaaacttc cgttttaact tcattggcat aagctacaat tccgcctttg
    48061 aaatattccg aacttcccgc atgagaagta aagcgatgag ctatgttgcc acctgtacaa
    48121 ctttcggctg tggcagtagt cttgccttgt gctgtcagca aatttccgac aatctgctct
    48181 ataggtaaat cctcaaatgc gatgatggct tttcccaaaa gttgtttcaa agattcgatt
    48241 tgctgattga cagcaaattc caatgaaagc aaatcgtctg atgtgcctgt cagtctgagt
    48301 ttgacgattc cataattcgg cagataggcc aaatgaataa aatcgggcaa ttctttttcc
    48361 cagtctgcaa gttttatggc caaagccgat tcggaatatc caaaaacctg aatcgttttg
    48421 tgcagaattt tttgtgtttg aaattttttc tgtaaacgtg gaataatttc gttgcgcatg
    48481 gcttccatca tttcataggg aacaccgggc atggaaacca ccactttttc gtcttgttca
    48541 aaccaggtaa tgggtgcagt tccaactttg ttttgaatga cggtacaatt ttcaggcacc
    48601 atagcttgcg agtaagtcaa atcgttgaaa acaaaatcga ttcggttggc aaaaacctgt
    48661 ttgatatttt ctattaccga aggatcgaac accaacttgg tatggaaata ttcgcacaat
    48721 gtttttttgg taatatcatc gttggttggg ccgatgccac ccgttatcag cacaatatcc
    48781 gttcgtttta aagcttcatc gagagcgttg gtaatatggg aggcttcgtc gtgaacagaa
    48841 gtaatttgaa atacttcaaa gccagccttg ttgagctctt gagccatcca tgccgagttg
    48901 gtgtccacga tttgaccgat aagaatttcg tctcctatgg ttatgatttc tacttttttt
    48961 ctgttcatgt tttctgtata attttatgta tattttttgt cagtaaaaaa tatctttaat
    49021 gtctaatttt tgcttgatcc gttgcatgca acggatcaac ataataacaa aatactgatt
    49081 atcaaaaaat tttcacgttt aactttaaat tttttagacg tagcatgcta cgtctctact
    49141 gaatatttat ttgctgatta tcaaataatt annnnnnnnn nnnnnnnnnn nnnnnnnnnn
    49201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    49261 nnnnnnnnnn nacttctttg taccattttt caccaaaacg acgtatcaaa gggactttta
    49321 aaaaacgata ggccggaatt ttcaactttt ttcccaaaac aaaagcacat tcgcaaactt
    49381 tccagtgatg cagattgacg gccgtgtaat cgttgatttt ttgcagtcta acaggataga
    49441 gatgacagga aatcggtttg caaaaatcgg tttttccctg ataataagct ttctccaaac
    49501 tacatttaca agttccatcc tcttcaaaga aggcaaaaac acattctctt tcattcacca
    49561 gagaggttac gcaatcgcca tcttcatcgg tataataaaa acctttttct cgaattattt
    49621 tttttgaatt ttccggtaaa aattcctgaa tttcaggaag aatatctttt attattttca
    49681 attcgtcttt ttccaaagga gcgccagcat cgccttccac gcaacaaata cctttacagg
    49741 aaaccaaatc gcaaacaaaa aattcctcca ataaattctc actgataagc gtatcattca
    49801 cttgaaacat agtattcaat ttttttgctt cgattgcaaa ataaacagta tgcaaagtta
    49861 atcaagttct tatttttcca gttcgattac ttcaagattt tgaatttttt ttccgtcgat
    49921 ttcgagacgt acgatggttt gaaccgcatg aaaaccatat ttaccggcag ctccgggatt
    49981 aatgtgcaac aagttgagct ttttgtcgta ctgaactttt aaaatatgag aatggccaca
    50041 tacaaataaa tccggaggtt gttgaaaaat agcagggcga accgacctat cgtattttcc
    50101 gggatagccg ccaatgtgag tcaaccatac cgccacatct tcgcattgaa aatgaaaatg
    50161 ttgcggaaaa agtgagcgaa tatttacatc atcaatattt ccccatactc cacgtacagg
    50221 tttgagctcg ttcagctgat tcactacttc caaagaaccc caatcgccgg cgtgccaaat
    50281 ttcgtcgcat tcggaaaaat attgatatat acgcggatcc aaataaccgt gtgtgtccga
    50341 aagaattcca atccgtttca ttttatttaa atatttgtaa taaagagagt tcgatattac
    50401 gattccatgt ttaaattata taaataattg ataattaata aggtaataag gtaataagtt
    50461 ttattcttgt tgttgatgat agttttatga atattaagac ctcatttcaa acaactgatt
    50521 ttagaaacat gtgaataccg cttttcatca accttatcag attaatttat aattgcttat
    50581 aaaatatttt tatgttgaac ttacgttata attattttgt gttaacgatt taaggatgag
    50641 aaaatttttt atcaaggata attttctgtg aatgaacaaa tcaaatagac tttcaaattc
    50701 gttagctttt acatgcaatg gctctatcaa atatttacac actgattatc agataattaa
    50761 taaaaattat actctgaact cttaactctt cacccgaaac tttttagacg tagcatgcta
    50821 cgtctctaca aaatatttac tcactgatta tcaaataatt aatcaaattt ttactctgaa
    50881 ctcaaaactc ctcactcgaa actttttaga cgtagcatgc tacatctcta ccaaaatatt
    50941 tattcgctga ttatcaaata attaatcaaa tttttactct gaactctgaa ctcaaaactc
    51001 aaaactccta actctaccat cccttcaacc gtaaaacatc tgtttttcat ttgtggctta
    51061 tttcaagaaa taaaattaat caaaaaagtt tagtattttt gctgtttttg aaaatcgtgg
    51121 cacattcgtt gttgtttttc ataaatatag atttacttct aattgaaaaa ttttttttgg
    51181 gaggaattga ctaaaaaaat ttttttaatt catcgaatta attctgtttt tttgaaaggt
    51241 ggatatgaaa atcaaattta ttttacatat atttttctgt tcttgtttgt ttacaacaat
    51301 tgaaataagt gctcaggaaa tagggaactt accagagaaa acgcctgaac aggaagccat
    51361 gcagcaaacc agtaaactac aacaggaatt ggatttaaac gcagagcagg ttaaaaagat
    51421 ttacgaaatc aaccttcggt atgccaaaca gcgtcagtcg gttacaaaac gttcggaagt
    51481 catggaactg ctgaaaagga aagatggtga aatagaaaat gcgctgggca aagaaaaata
    51541 tcagcagctc cagaacaaac gcaacgaacg aatttccatt gatgtcccaa ctacatcggg
    51601 tatgcaaaat attacgcctt cctatcgttc caccaaaaga accggaaata cgaatttgag
    51661 ttttcccgaa agaaatttgc aaaacgacag caaaacttca gatggtaata cggaacaacc
    51721 caaacgtttg actcccaata attcgaggcg taccactgat gaagtggaaa gaaaaaacac
    51781 tccgcctcaa caaagaaagt aaagatacat gtcgcaccct ttctttcagt tcaaacaatt
    51841 cactgttttt catgatcgtt gtgccatgaa agtgggaatt gatggcgtaa cgcttggagc
    51901 ttggacaccg gtagataatg caaaaaaaat tcttgatatc gggacgggca ccggcttaat
    51961 agctttgatg ctggctcaac gaagtacagc tgaaattgat gcgttggaca ttgataaaca
    52021 agctattgaa caagcccgtt tcaacgtgga aaattccgca tggaaaaatc gcattcgttt
    52081 gtttgaaact tctctgcagg agtatcattc agtttgtaca acggcttacg atgtattggt
    52141 atgtaatcca ccctatttca taaattccaa aaaaagtcct attgttgcca gaaatatggc
    52201 tcgtcatgca gaatctctta cacatgaaga gctgattgat ctttcctttg atttgttgaa
    52261 accggacggg acattgtgtg ttattctgcc ggtggaggaa ggtttgcagg caatttcaca
    52321 tgctttggcc agcaattggt atgctcatca aatggttttc gtttttccca agcctgcagc
    52381 caaagccaag cgattgctta ttcagttggg taaaaaagaa aaaatcaccc gtatttcgcg
    52441 acttgccatc gaaacggagc aacgccacga atatacagat gaatacaaaa atctggtgaa
    52501 aaatttttat ttgaaactgt agtagttaaa tcagagaaaa aattaaatca aagatttgat
    52561 tttttcggcc aagactgatt tggaagttgc accaacttgt ttatcgacga gttgtccatc
    52621 tttaaaaaac agtaaggttg ggatattacg aatgccaaat tttgttggaa cttcagcgtt
    52681 ttcatctacg tttaatttcc cgacttgcac ttttccgtca tattcttcgg ccagttcttc
    52741 tacaatagga cctaccattc tacaagggcc acaccattct gcccagaaat caaccaatac
    52801 gggttttcca ctttcaataa attcgttaag attttcgtct gtaaattcaa tgaccataat
    52861 ttttaagttt tgaagtttgc aataattttt tattcaaaaa acaaatatcg gtaacaaaag
    52921 ttttataatt aaacactatt ttgctataga aataatcaat taaagataac attgacttat
    52981 caatttacat caaaatcgag tattccttca cttttggcag ttttcagcat tcgatatact
    53041 ttggggttga tatccacgcg atgctgtcgg gcaaaaaagt tgattttatt gaaagaattt
    53101 tgatcttgta cctgaatata tacatttaca tttcccttat ttccaagaat aattttactt
    53161 aacatttcaa cggtatcttc cgtaagttgc tgcagtggga tattaatttt caggttattg
    53221 acgaatttgt ctttaatatc tgaaaaaaaa tcgatttttt gtattttaag ttcaagttct
    53281 tgttctccgc caatatctgt atttttcctg tatttgctgt cggcatattt ttcctgaacg
    53341 atggcctgaa tgaaaacaaa catgtctttt atcatgtatt tctggaattc tatataattg
    53401 ttgccataca acacaaattc ataggaacca tcgtaatctt cgagagtgaa tacgccgtag
    53461 ggttttcctg cttttgatac ggaatgacgg atattggtga cgagtccgcc aattttcagt
    53521 tgtttccctt ttagtgaagt caaatctttc aattgtccgg tagaagtgtt gcagatgtaa
    53581 ttcatttcaa attcaaactg atcgagtggg tgagcagaaa ggaacatgcc gatcaattct
    53641 ctttccctgt ttaatcggtt aagaggagcc cacgccggcg catagggaat ttcaggcttg
    53701 ttgatttcaa tggaattcat atcgccaaac agtgtatttt gcgacataag tctgtcggct
    53761 tgatatttgt tcccgtaacg aatcaagctt tcgatcaccg tttctccttt gccgttttcc
    53821 acaaaaaact gttcgcgttt gatgtcgttt agtccgtcaa aagctccggc tattgccaga
    53881 ctttcaatag cacgtttgtt gcaggccgta agattgacgc gttccacaaa atcgaaaatg
    53941 tttttaaatt ttccgtgtgc atgtcgttcg ttcactatgg ccatgacggc attttcgccg
    54001 acacctttga taccgcccaa accaaaacga atatcgtttt tctcgttgac agaaaaagta
    54061 aaatcactct cattgacatc gggacccagc acattgatgc ccatatttcg gcattcgtcc
    54121 ataaatttgc tcacttcatc gatgtcatcc ttatttcttg agaggttggc tgccatgaat
    54181 tcggacggat aatgagcttt caggtaagca gtacgataag cgagataaga gtagcaggta
    54241 gcatgcgact tattgaaggc ataattggaa aattccaccc agtcgctcca aatttgttcc
    54301 aatttttcga gcggtccgta gccatttttt tggcaaccag caataaattt ttccttcaaa
    54361 gcctgcattt tgccggtgag ttttttcccc attgcttttc ggagttcatc ggcttcagca
    54421 cgtgtaaagc cggccaaatc gacactcagc aacattacct gttcctgata aatggtaata
    54481 ccgtaagttt ctttcagcct cttttccatg atgggaaacg gatattcaat cttttccaat
    54541 ccatgttttc ggttgatata ctgaggaata tactgcattg ggccgggacg gtaaagcgca
    54601 ttcatggcaa ttaaatcttc aatttgtgtg ggctgaagag cttgcaaatg tttttgcatg
    54661 ccggcagatt caaattgaaa agtacctaca gtttgacctt tgctgaaaag ttgataggtt
    54721 tcaggatcat ccaatggtac tttgtcgaga tctacttcga tatttttgga ttttctgatg
    54781 ttttccaatg tatctttgat aatggaaagc gttttgagac ctaaaaagtc catttttatc
    54841 aatccgactt cctctatgac cgaaccttcg tattgcgtaa ccagaatttc ttcttttgtg
    54901 tccttgtctt cggcagtgga taatggaaca aattttgtca ggtcatcggc accaatgatt
    54961 atgccacagg catgaactcc ggtttgtcgt acagtgcctt ccagcatttc ggcatagcga
    55021 agtgtgctgg aaagattttc gtcggaagaa tttcgggcaa tctccaattc gggaacaaat
    55081 ttgaaacaat tggacaaatt gactttcatt ttttttccgt ccggcgtatc gggcaatttg
    55141 tcgggaataa gtttggtcaa tctttctgct tcggaaagtg gcaatttttg aacccgcgcc
    55201 acatctttta tggctgattt tgctgccata gtgccaaaag tgataatatg ggcaactctg
    55261 tcctttccgt atttttcggt aacccagcgt aaaacttcat ttcgtccgtc gtcatcgaaa
    55321 tcgacatcaa tatcgggcat ggaaatacgg tcgggattca gaaaacgctc gaaaagcaaa
    55381 tcatatttca aaggatcaat atcggtaatt ttcaggcagt aagctacaac cgaaccagct
    55441 gccgaaccac gacccgggcc aacggcaaca cccatatttc ttgccgcagc aataaaatcc
    55501 tgaacaataa gaaaataacc gggaaatccc atagttttga taacgttcaa ctcaaaattg
    55561 atgcgttctt caagttcggg atccagattt tctccatacc gttctttagc accttcaaaa
    55621 gtaaggtttc tcaaataatc ggcttccagc ttgattcgga taaccttgtc catgccaccc
    55681 aaacggtcaa atcccttacc aaattcttca cgcagcatgg cttcggtgta acgtgagcgg
    55741 tattgatttt catcggcaaa ttcttccgga attttgaagg ttggcatcat gggtgccgaa
    55801 tcaatatcgt aaaactctac tttttgagct atttcgagtg tattttcgag cgcttcaggt
    55861 atgtccgaaa atatttcctg catttgttga ggcgttttca accattcctg tttggtgtaa
    55921 cgcaggcgtg tgggatcgtc catgtctttt cccgtactga gacaaatcaa tcgttcatgt
    55981 ccttcggcat gttcttcttc cacaaagtgg acatcgttgg tggcaatcag ttttgtattg
    56041 gtttttctgg ccagctttat caattcaaca ttttgaaggg tttgtttttc gtaggtagaa
    56101 gtgtcgcctc cgggtttgtc ggttttatga cgttgcagtt cgagatagaa atcgtcgcca
    56161 aatacttttt tgtaccaaag aacagctttt tctgcttctt ccaagtttcc cgattcaatt
    56221 tttttgtgaa tttcacctcc cagacaagct gaagaagcaa ttaaaccttc actgtatttt
    56281 tccagaattt catggtcgat acgcggtcga taatacattc cttctatcca agccagcgaa
    56341 atgagtttgc atagatttcg ataacctgtt ttatttttgg ccaacaaaat gagatgataa
    56401 ccactccggt cttcctgact ttctttcgag aaacggcttc tatgggctac ataagtttca
    56461 catccgatga ttggtttgaa aagtttacgt ttttctgttt caatattttt atgaatctca
    56521 tctttttgaa tgtcagataa ttcatcttta tttaattgct tttgcaaaag ggaaatattt
    56581 tcataaacag gaacattttt cttttttacg tagttgtaaa attcttttat tccgaacatg
    56641 ctgccgtgat cggtgagtgc aatagccggc atgccggttt tcatagcttt gtcgaccaat
    56701 tgatctatgg aagacatgcc gtcgagaata gaataatgcg agtgaacgtg caaatgaaca
    56761 aacgtattca tgctaaatag gtgaagtatt gaatattaga aaagctgaaa aactttaaat
    56821 ttaggtgttt aaagttagta aaatttttgt tgtggacaga tttatatcga aggaaaaatt
    56881 tcaaaaaaag gaaataaaat ttattctctt ttgattttca acagtgtctg aaaaataaat
    56941 caggggctgt atgttaataa gcccctgaaa aagaaatatt aaagaatttt tcaagctcca
    57001 aaatcgtcga acatcagcat ttcacgcggg acgccgagat cgtcaagcat tttttcgact
    57061 gctttggcca ttggacccgg cccgcacata taatattcaa tttcttctgg ttcatcgtgg
    57121 aatttgagat attcattgag aataacgcta tgaataaatc ctacaggccc agtccagtta
    57181 tcttcgggaa gaggttcact caaagctatg tggaacgaga aattgggaaa ttctttttcc
    57241 agttcacgga aatcttcttc gtagaaaatt tcgtttttcg aacgtgcgcc ataccagtag
    57301 gaaatttttc ggtcggttat gcgtaaggtt ttcagcaaat gtaaaatatg ggagcgcaaa
    57361 ggagccattc cggcgcctcc accgatgtaa agcatctcgc gttttgaatc gataattgga
    57421 tgaaactcgc caaacgggcc ggagacattc actttatcgc ccggtttaag acggaaaata
    57481 taagaagaag caatacccgg ttttacatct aaaaattttc ctgtagctcg gtcaatggga
    57541 ggggtggcaa tacgaacatt cagcatgatg atatcgcctt cggccggata attggccatt
    57601 gaataggcac gcatggtttc ttcgtcattg ctgcatgtta aatcccaaag atgcatttta
    57661 tcccaatcgg gacggaaacg ttcttccact tcaaattcac tgaatttgag ttcgtatcgt
    57721 ggaatatcaa tttgaatgta gcttccgggc agaaaattca tatgctctcc ctcaggaagt
    57781 tttacaatca gttctttgat aaaggtcgca acatttttat tggaaatcac ttcacattcc
    57841 cattttttaa tgctgagaat ggattcggga atttttattg aaaggtcatt ttttaccttc
    57901 acctgacaac ccaaccgcca atgttctttg atttcttttc gggaaaaatg tcctgtttcg
    57961 gtgggtaaaa tttcgcctcc gccttcgagt acctgacagc gacattgagc acaagttcct
    58021 tttccgccac aggcagaggg tagatatatc tgctgattga ccagtgtact caacaaagtt
    58081 cctccggccg gcacttccag ttttttttca ttattaatgg tgatagtaac gtttcccgaa
    58141 gcgacaagat attgtttggc aaccaataaa accactacca ataaaaatat aactacaaaa
    58201 aataccaata agctggcaat cagtgttgaa gtttgaattg ctaataacat aatttaattg
    58261 acgttatcct tcccgttttt tttgaaggaa taatttattg attaattaat atctattgca
    58321 aataaaatgt ataaaataaa aaaacagaag attttaaatt aaattttcaa gcccgagaaa
    58381 cacataaagg caatccccat taaacctaca gttataaaag ttattcctaa accacgtaac
    58441 ggtttcggaa cattggagta ttccattttt tcacgaatgg ctgccagtcc gacaatagcc
    58501 agcaaccagc cgacgccgga acccaaagcg taggatgtag cttcgccaat attgtgaaaa
    58561 gctctttgct gcataaatag cgatgctccc attatggaac aatttactgc aataagtggc
    58621 agaaaaattc ccaatgagct gtagagtgaa ggagaaaatt tttcaactgc catttcaaca
    58681 atttgtgtta tggccgctat gacggcaata aacaaaatga aagagagata actcaaatcg
    58741 agtgaagcca attgtggact aagccactgc aaagctccct cttttaaaac gtaattttgg
    58801 agtaaataat ttacgggaac ggtaataaaa agaacaaatg taacggctgt ccccagtcct
    58861 aaagatgttt ttacgttttt agatactgcc aaataggaac acattcccaa aaaataggca
    58921 aatatcatgt tatcgacaaa aatcgattta acaaatatat tcaatatact ttccatattt
    58981 tatttgttgt tttcttgata atatgaataa ttattcaatc gttttagtag tcataaaaat
    59041 tatatgacta tttttcttgc aactccttgt ttttggctct atgaacccaa atgatacatg
    59101 ccacgagaat caacgccata ggaggcatca tcatcagtcc gttgttcatg tatcctacgt
    59161 taaaaaatga ttggggaata atattatatc ccaatagtgt gccagaacca aaaagttcac
    59221 ggaaaaatga aacaataatt aaaataatag catatccaaa tccattgcct aaaccatcca
    59281 aaaaagattc ccagggactg ttggacatgg caaaagcttc cagtcgtccc attaaaatac
    59341 agttggtaat aatcagtccg acatacaccg acaattgaac actaacgtcg taggcaaaag
    59401 cttttaaaat ttcgctgact atggtaacta atgcagcaac aactaccagt tgaacgataa
    59461 tacgaatacg attgggaatg gtattacgaa caagcgaaac aataatattt gaaaaagcaa
    59521 ctatgatggt aacagaaatt cccattacca aagcaggctc gagttttgca gtaactgcta
    59581 atgccgagca aatacccaat acttgaatcg tgatgggatt attcttgctt aatggtccca
    59641 ataatacttg tcttgacttt ttggataata atgtactcat tgttttattt ctcctatggt
    59701 tgataattga cgaaaaaacg cttcatattg tcctatacag ttcagcagca tggtttctac
    59761 acctttgctg gtaattgttc caccggaaat agcatctact tgttcccttc cttcagcttt
    59821 tttaccgact ttcataaccg caatcgacac aaattcattt ttttcgttaa aaatatgttt
    59881 accttcaaat tgtctttgaa aattcggctc agcaatgttg gcacccaaac ccggtgtttc
    59941 actggcatga gaaaaatatg ctccgtaaat agtattctta tcatcgttta gtgacacata
    60001 tccccaaatc ggaccccaaa gacctgttcc acgcaaagaa atgatatatt ttgtttctcc
    60061 ttcgacttgt gctataaaaa ttggaagtcg cctttcttca actttttttg ataattcttt
    60121 ggcaatgtca atatcaaaag cttctttttt tgagtaggat aaaatctcgc cattggtatt
    60181 gacaattaaa cctttaataa catactgatc atatagttgt gctatattgt tctgtttagc
    60241 atcgatacgc aaagatttta aaatctgctt tttcttgtcc aattctatat tggaattttg
    60301 cttttccttc agcgctcccg atacaaatgc cagcatgagg gcaactatta ccaccattac
    60361 tgtagcataa accagtgtgt aagtattact gttcgtattc attttaatgt atattatttt
    60421 tttgggttat tgctgatccc atcattactt tattgaatgg atttttgtgc tctacttatc
    60481 cgttttttta cattggcatc tacaacataa aaatcgataa gcggagcaaa aatattcata
    60541 agcaaaattg ccagcatcat tccttccgcg tatcccggat tgagtactcg aataatgatg
    60601 gccaggcatc ctattaataa accgtatatc catttccctt tttcggtacg ggctgaagtt
    60661 accggatcgg ttgccataaa taccgcacca aaggcaaatc caccaaaaac cagatggttg
    60721 taccaaggaa aatgtgcagc agcagtatca ggcccaaata gattgaaaac aatgcccatc
    60781 aaagcacctc caaaaaatac agaaagcata gttttccaac tagccacttc ggttattaac
    60841 aaaacaaaag ctcccagcat gatcgctatt atagaagttt ctcccacaga accgggaatc
    60901 aggccaacga atccatccca aaatcccaat gagtgaccaa aagtgtccgt tatatgggga
    60961 acagaagttg aagcagtagc tatctgcccc aaaggagttg cccctgaaaa agcatctaca
    61021 acatttccgc ctccaaatcc caaagcagtt ccggttcgta tccaaacgct gtctccggac
    61081 atgtgagtag gatagccgaa aaataaaaat gccctggcta ccagtgctac attaaaaata
    61141 ttcattcctg ttccgccaaa tacttctttg gcaaaaatta cggcaaatgc ggtagctact
    61201 gctaccatcc aaagcggagt gttaatggga ataattaatg gaataagaaa acccgttacc
    61261 aaaaaacctt cctgtatttc ctcacctttt atttgtgctc caataaattc tattcccaag
    61321 cccactgcat aagaaactac gatatacgga agaacggcca aaaaaccgaa taaaaattcg
    61381 gtccaaaaat ttacatcctg acctattgca aggtagtgtt gataaccaac attatacatg
    61441 ccgaacaaaa gagcaggcac taaggcaaca atgactgtac tcatggttct cttggtgtct
    61501 actggatcgt gaatgtggct gccgtattta ggtgctgtgg tagtaggaac aaataaaaat
    61561 gtctcaaacc catcataaac agaatgaagt ttttctaact tgccgccttt ttcaaagtac
    61621 ggttttattt tatccatgaa atttcttaat gcgttcatat ccaaaattat ttctgctttt
    61681 ttgttgccgt ttaaacggtt gtttttatga atttttttat tccagttctt ttttcatcat
    61741 atccaaagct tcacgtacaa ttagctgcag aggcaatttg gaagtacaaa caaattcgca
    61801 caaagcaaca tcttcgggtg ctatttcgta accgcctaaa gcctccattt tatcaatgtt
    61861 tccggtaatc atggctttaa atagatattc cggcaaaata tccataggca gtacccgatc
    61921 atattcgttc gacataataa tggctcttct tccgcccaaa atgcgtgcat cataagaaaa
    61981 tttgatatct ccaaagattt ttttgaaaaa tttcttttgt aacaggggag taaaaaataa
    62041 tttggtagca ctgaatttat tcaaacgggg catggcccag cccaaaaatt catgaatttc
    62101 tgttccttca tctataacaa aaatttgaga agcgtaaggg tcaataaagt cattaatatc
    62161 aacttttatt ccacttatta catttcccaa aatatatctc agaggaatac ctttatgtac
    62221 gttaccgttt ataaaaggag ctactgctgt tccgggcagg gtagtgtaat attttggttc
    62281 aattacttct ggaccagtta aagctataat tttttttaaa tcaatgatgc ccttattaaa
    62341 aaatctgcca atgtaaagca tatcctgaac attgacagtc caaacaacct cacctttgtt
    62401 aatgggatcg acgtgattga tttgtattcc tacatttcct atgggatgag gtccttcata
    62461 ttcagtaatt tcaacatttt ttactttcaa aaattcgttt gaaggaacat tggcttttac
    62521 gcccaaatga acttttcccg gtgtcaattt ggccaatgca tcaattcccg tttgaaagtc
    62581 tgcaaactgc cctttcaaaa taaaattgta atccggagcc agtggagccg tatcgaaaga
    62641 ggaaataaaa attgactttg gcgttttatt gggattggct attacatcat aggggcgttg
    62701 tttgatgtaa ggccaaattc cagctttcaa caaggctgat ttgatttcct ctccacttaa
    62761 actttccaat gatggatgtc caaaattttc atattcgttg acaacatcac gctctattgt
    62821 aatatttatc aattttcttt tctcacctct gtttacagcc aatactttgc cgctaacagg
    62881 cgatacgatg tttaaatttt cgtgaatttt atcgtgaaaa accggtgtgc cggcttttac
    62941 acgatctcct gcccgtacta atacttttgg aattattcca tgataatagt ccggaacgag
    63001 tgcgacaatg tcagaaatca cggtacgctc aatcatcttt tgagcagcac catctatagg
    63061 gatatccaaa cccttggtag tcttaataac attagtcata aaaagaaaaa tttggtttta
    63121 aaattttgga taaaattatc cctcaacttt tttgctaaaa tatttgtcct gaaaaacggt
    63181 gcaaaattac acatttttat tgttattacg gagagtttat tggagaaatt tttcattaat
    63241 ttttcgtggc ttctttaaaa ttgtaaatgt tatatacgat ttttatttca gaaatattat
    63301 aatgaatcaa taaatatagg aagaacagtg ttttgcggat aaagttggat taattttatt
    63361 atttgagtat aatttgaaag gttttcagaa ttaattggtt tttaaacggc attatagaat
    63421 gattcggcca aaagcattct tagacaagac aaatgaagaa atccggttac taatttgtag
    63481 gaggaaattt catggatttg gcgtaaacat gcaatgaagg gtaagagaag gatattaaat
    63541 tttgtgtaaa ttaaattatc atgtaaattt gccatctgtt tttcataaaa actatggatt
    63601 ggaaagaaat ttatttacaa gcaataaatt ggcgcgaaaa acacatcaaa gaacgccagt
    63661 ttatcctgat attaagtttt ctgacaggaa tttttgctgc gttggcagct tttgttctaa
    63721 agatggccat tcattccgtg cagtcattgg taacagaaag ctttaatcgt gaaagcctca
    63781 attatcttta tttaatcaca cctgcagtcg gtattattat tacatctcta tttgtcaggc
    63841 atatagtaaa agatgatatt agtcacggcg taacacgtat tctttacgcc atctctcaac
    63901 gaaaaagcat ccttaagttg cacaatgtgt ggacgtcatt ggtgggcagt tccattacca
    63961 ttggatttgg tggatcggtt ggagccgaag cgccaatagt tttgaccggt tcggccattg
    64021 gttccaattt gggaaaattt ttcaaaatga atcaaaaaac catgatgctc ctcatcggtt
    64081 gcggtgctgc cggtgctatt gcgggtattt tcaaagctcc cattgccggg ttggtcttta
    64141 cgctcgaagt tttgatgctc gacatgacca tgacttccat tgtacctttg ctcatttcgt
    64201 cggttacggc aacctcggtt acctatattt tgacgggcga tgaattcatg tttcaattta
    64261 ccaactacga accttttgcc gttgaacgca ttccttattt gttgatgttg ggtgtagttt
    64321 gcggattggt ttcgttgtat tttacaaggg gcatgaactg gctggaaaat ttttacaaaa
    64381 agtttgaaaa tccttacatc aaatgggcca ttggcggaag tatactgagt gttttgattt
    64441 ttctctttcc tcctctttat ggcgaaggat acgataccat cgcctccttg ttgaataatg
    64501 acatttcttc ggtagtcaaa caaagttttt ttctcggttc ggaaaacaag cctttggttt
    64561 tactcaccta tttggtgctg attattgtat ttaaaatttt tgcaactgcc tctaccacaa
    64621 gtgccggtgg tgttggaggg atttttgctc catcgctttt tgtgggttgt attactggtt
    64681 ttgttgtggc cgaaattctc aacctgatag gtttgaatat gccgatagaa aacttcgcat
    64741 tggccggtat ggcaggtttg atgtcgggtg tgatgcatgc tccgctgacc ggcatttttt
    64801 tgattgccga gcttaccgga agttattatt tgtttatgac gctgattatt gtttccacca
    64861 tttcttatct caccattatt cttttcgaac cacacagtct ttatgccatg cggctggctc
    64921 aaaaaggcga gttgattacg cacaacaaag acaaggctgt attgaccctc ctgaaaatgg
    64981 aaaatgtaat agaaacaaac ctgaacgtat tggaaccgga catgaaactg ggagaagtaa
    65041 ttaaaataat ttcgcattcg aaacgaaatg tttttccggt ggtaaatccg aaaaatggag
    65101 ttcttttggg gattttaacg ctcgacgaaa tcagaaatat catgttccga cctgaattgt
    65161 accagcgttt cacggtacgt cagctcatga tttcacctcc gacttttctc aatgtgaaca
    65221 tgccaatgga aaaagtcatg cagatttttg aagacacggg agcatggaat cttcccgtcg
    65281 tggacgacga aaaacattat atcggatttg tttcaaaatc caccatattc aattcttatc
    65341 ggcatgttct ggtacatttt tccgatgaat aaccgaagaa tttattatcg acaaacaacc
    65401 aacatttaaa ataagatgac caaaaacaaa cctacttacg acgaagccat tgctgaattg
    65461 gataaaattc ttgcagaact ggaaaataac tcggacatca acatggatga aatagctcaa
    65521 aaagtaaaac gagcttccga attattgcaa atatgtaaga aacagctgca cgtactggat
    65581 gaaaatctgg aaaaactcct ctcagagttg gaagaataat tatttgacaa gtgtaatatt
    65641 cacttctttt gcttcagcat atttttcgag caacattctg gcctgctcgg acgcttcttg
    65701 caaccctttt tctccgctat aaccattgat aatcattaac agacggcgag tttccattgt
    65761 taattttgtt cgttcgttgt cgctggtagg cgttccgata cctccttcag catctttgaa
    65821 aagcggtaat ccttccacat tcaaaatacc gcgaccaata gcttgaaaaa tatcgcttgc
    65881 cgttcccact tccagccgaa ggtctccctg aatttttttt gcatcaaatc ctccaatgga
    65941 aaatccgctt cggatggaaa tcaggttgat gatatccacc aatgtgctta ctcgctgaat
    66001 acccactcct ttgcaaattc ttctgcacag cgcttcggcc gaaggacgat agcgattggg
    66061 atcctttccc aactttctgt aaacttggcg cgtagcggaa atttcgggac gcatttttat
    66121 attttccaat gtaaaagttg aagaaatttt ctccatttcc tcttctatct cttgccacaa
    66181 aatacttgtg gtttcggaat ttttcacttc gcaggcgata acgcttaaag tcaaatcggg
    66241 acaagcctga cgaatggatt cgctaatgtt gatctgcata ggattatgct tgttttaaca
    66301 tgatgccgct caacattcgt ccgcatagaa taccttgacg ccgggcagaa aaaaagtttt
    66361 ccgattcggt gaatgtacag cgacacagaa actcaatatt atttttttgt aatccgcttc
    66421 ccagtaatga tatttcgttg gctttccata aattgagaaa aattttccca tcattttggg
    66481 agaaaatgtc cgacaaattc caatcgtttt tctgaaattc ttctaccaca tccctgccaa
    66541 cttcatatac cgaaggcgaa atggaaggcc ctgctaccac aaaaatatct tccggcgaaa
    66601 ggtcaaattt accaaccagc gtttcaatgg tttttgcggc tattccggca caagttcccc
    66661 gccaaccggc atgagccaca gcaatcactt ttttctcggg agcatagaac agcaatggca
    66721 cacaatcagc agtagtaatg ctgataacta ctccgggcaa ttgagtaaac agtgcatcaa
    66781 caccattgag aaaatgttgc ctttgctcgt gcgataatcc gaaaaatgaa cgatcaattt
    66841 cgcggatttc gcaatcgtga acctgatagg gaaatattat gtcggaagtt ccaattccca
    66901 attgccggga caatatttca aaatttcggt gcacattttc ttcttcatca cccacatagg
    66961 gactaagatt gaacgaagtg tattcaccac cacttacccc accatgacgc gttgtgaaga
    67021 aatgtgttat ttcttcgaaa ggctgcagta tttctgattg aataaacaaa cgcattgaaa
    67081 atcgatttat aataagtaaa ttatctctca gagaagaaag aaaaatttga ttttacgcca
    67141 gaaagacaat ttaattttgc tgaataatta tataaaatat gcgaaaattc ggtaaattca
    67201 ttgctcctat tcatctttat cactaatcaa aa
//
LOCUS       DAOSIB010000002        55536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_671_length_55536_cov_11.125052, whole genome shotgun sequence.
ACCESSION   DAOSIB010000002 DAOSIB010000000
VERSION     DAOSIB010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 55536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55536
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_671_length_55536_cov_11.125052"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1213)
                     /locus_tag="PLD44_00325"
     CDS             complement(140..1213)
                     /locus_tag="PLD44_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOF97816.1"
                     /translation="MMASKINPAFQDKLKKYGAVNFNACYNCGTCTAVCSLSTPEESF
                     PREMVRLSSLGLENDIKSSLKPWECYYCGECTENCPQTANPGELMMSLRRWLTSVYDW
                     TGLSGLLYRSVTFTISALIMVFIAVLVLGNAMSFQLAEMMHYGHLFEMYVVFAVLAII
                     FIPNIARMWNFTILRPKVKANLKIYIKSLPDLFVHMFTQKRARDCDKEDNFRWLEHFI
                     LVLAYLSLLFTTVFLDWFGTKSFFVVVLGYVESFFVFVITADFVSSRIRKAKALNKFS
                     QPSDWLFVIWLMLMGLTAFLVRLFIDTHMIDNNVWMYIVHLTVLAQWAIIIVPFGKWS
                     HFLYRSFGMYFSSIKAASEKRKG"
     gene            complement(1210..1491)
                     /locus_tag="PLD44_00330"
     CDS             complement(1210..1491)
                     /locus_tag="PLD44_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97817.1"
                     /translation="MMEAEIGKTAKYLRDKQGIPEKAKENLKEFVRIKKALIDALKNG
                     EMTIPQLSEKLSMSKEDVTFYLMTLVKYGDVAVGEIDDNDEYFTYKLNK"
     gene            complement(1496..3223)
                     /locus_tag="PLD44_00335"
     CDS             complement(1496..3223)
                     /locus_tag="PLD44_00335"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF97818.1"
                     /translation="MKKNLGVYICHCGGNISDYVDVEKVRQAIEHEEGVVLAKTTMFA
                     CSDSNQKEMVEDIHQYQLDGVIVASCSPKLHLVTFRNVTETAGLNKYTYVHANIREQA
                     SWAHSDDKEGATEKAIRLVRAAISKSRYAEALYPNKFEAEKAIAVIGAGMAGMKAALS
                     IAEKNMSVVLIEKENTVGGHVAEWGSLFMTNEKGSELVQRIHNDLLQHENISILTGTE
                     VVSSKGSIGNFTLTLQKKHQPVDNLRETMEINVGSVVIATGFDHYIPQRGEFSYLNNE
                     NVISLPDFKKLTDQSTGKTLLYNGKEIHDIAYIYCVGSRQSEGENKYCSRFCCISTIH
                     TAIETKKKFKGIRNFHLHRGIRTYGKQELLYDEALQNGDIFIQFHDDFPPEVSFKNKK
                     TSLKVKDLLFSDKELLLDADLVVLVTGMLPRIDQSIGQLFKLPKGRDNFYNEIHMKLR
                     PVETVIDGITLAGTCQGPKNISESVNSALSAATKAFSSISNGLVETEPIVAEINGNSC
                     VWCGECLKACPFEAIEQKEIDGKIIAVVNKSVCKGCGMCAPVCVPNAIDLIGYSDKEI
                     FSMIDALVQ"
     gene            complement(3232..4530)
                     /locus_tag="PLD44_00340"
     CDS             complement(3232..4530)
                     /locus_tag="PLD44_00340"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOF97819.1"
                     /translation="MEERRKKIEFDALVIGAGIAGGETALNIANNGYKVLLVEKDHSI
                     GGKMILLSKVFPTLDCAACITTPKVSEIARHPNITVFTESEVTSIVKKTESLFIAKIT
                     KNPRYVHVEDCTACQLCEKACPVSVRDEYQFGLVGRKAAYIPFSIANPKAAVIDLEQC
                     TLCGACERVCPTHCIDFTMETECFELQVKTVVLATGFQLFDPLKIPRFGYGKYKNVVT
                     SMQMEREIAPTRPYHTILRPGDGKIPDQIAYVLCTGSRDDSVGNPVCSQVCCMYSMKQ
                     AQLLMGALPMADITIYYIDIRSFGKGFEEFYQQAKDMGVNFVKGKIGKISEKENGNLI
                     LRYEDIQEGIVKEAEHDMVVLSVGMLSNQSVHQIFDGEMLQFDSLNFIWQSDLLGSPA
                     KTNIDGVFVAGTASGPMDIPDSILSAGSASSEAISYLIQH"
     gene            complement(4534..4947)
                     /locus_tag="PLD44_00345"
     CDS             complement(4534..4947)
                     /locus_tag="PLD44_00345"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOF97820.1"
                     /translation="MTSFVKKNAKILVFSTEKISDPAIDMAGLLKLHYPPTVYTITVP
                     CSSGIKPNWILHAFEKGFDGVFIAADGTDCVFGESCTEKTGKVVAMTHEKMKQNNLDP
                     ARLRMAALCSVCSDAFVRQVRSLNEYLIKTRYSEK"
     gene            complement(4959..5210)
                     /locus_tag="PLD44_00350"
     CDS             complement(4959..5210)
                     /locus_tag="PLD44_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOF97821.1"
                     /translation="MTTEELKNLTVAKSVDARGTACPGPLLEAKKAIGTIQSGEILEV
                     LSADEGTKNDLPKWCNKQGNEFLGMVEENGYYKLYLKKK"
     gene            complement(5249..5818)
                     /locus_tag="PLD44_00355"
     CDS             complement(5249..5818)
                     /locus_tag="PLD44_00355"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOF97822.1"
                     /translation="MEKSNEITMEKLLAELENLKAKTTQLENSAKKDQLSIAIISGDM
                     DKIMAGMIISLAAAAMDTKVKLFFSFWGLAALRDPKKTVKGKNFVSKMFGCMLPKGYG
                     KLKLSKHNMCGMGPMMMKNLMKKQHVLSLDEMFKEAAELGIEITACEMTMNLMGFKKE
                     EFIDYPHFRIAGAATFVADAGESAMQWMI"
     gene            complement(6019..6741)
                     /gene="recO"
                     /locus_tag="PLD44_00360"
     CDS             complement(6019..6741)
                     /gene="recO"
                     /locus_tag="PLD44_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOF97823.1"
                     /translation="MQVKTEGIVFHSLKYSDNSAIVTIFTRRFGRVAYMIYGRGKRKS
                     SWKPAFFQPLSIVELEVSHDMRKNIQQMKDVRVLVPYQSLTIDPLKRSIAMFIAEVLL
                     KVLSQSEADESLYLFLRQSLEFLDNSKQKTANFHLVFLLKLSRYLGFEPNIDGQTTLF
                     FDLVNGVFLSSPPAHRHYLFAEEAGKMAQLLQTDYEHMTEIKLSRQQREKLLEDIVSY
                     YRFHLPDFPEIHSLEVLKTLFD"
     gene            7036..7110
                     /locus_tag="PLD44_00365"
     tRNA            7036..7110
                     /locus_tag="PLD44_00365"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7069..7071,aa:Glu,seq:ttc)
     gene            7141..7395
                     /gene="rpsT"
                     /locus_tag="PLD44_00370"
     CDS             7141..7395
                     /gene="rpsT"
                     /locus_tag="PLD44_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOF97824.1"
                     /translation="MANNNSAIKRIRQANKKRLHNRYYIKSTRTAIKRLRTTTDKEKA
                     AELLPQVSSMLDKLAKRNMIHKNKANNLKSKLAIHVNKLA"
     gene            complement(7452..8105)
                     /gene="upp"
                     /locus_tag="PLD44_00375"
     CDS             complement(7452..8105)
                     /gene="upp"
                     /locus_tag="PLD44_00375"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOF97825.1"
                     /translation="MKIINLSDNNSILNQFIKEIRSVEIQKDSMRFRRNMERIGEIMA
                     YEISKAMDYVEEKVQTPLGIAPVKVIDEKVVISTILRAGLPFHQGFLNYFDGSENAFV
                     SAYRKYRNAENFEIFVEYIASPGLEGKTLILTDPMLATGSSMEQTFSALTTKGFPSKI
                     HIATVIASRHAINYLTTHFPENITTIWAAAIDPEINSHSYIIPGLGDAGDLAFGEKL"
     gene            complement(8167..9822)
                     /locus_tag="PLD44_00380"
     CDS             complement(8167..9822)
                     /locus_tag="PLD44_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOF97826.1"
                     /translation="MLEKYLKQTEFKDFDDFKANYQLIVPEHFNFAYDVIDEWAAKEP
                     DKLAMIWTNDQGECRKFSFAEMKKYSDQTASYFQSLGIGKGDVVMAILKRRYEFWFTI
                     LALHKLGAITIPDTHLLTPKDLIYRNNAADIKAIVAVGEEPIVSHINDSMAESPTVEM
                     LISIGPIVPENWLDFHKGIDEATPFTPIHHVNENTDPLIISFTSGTTGNPKMVLLDCL
                     YPLGHIITAKYWHNLHENSIHLTIADTGWLKALWGKIYGQWIVGACVFVYDHEKFTPA
                     NMLEMIEKYHVTSLCAPPTIFRFLIREDISKYDLSSLEYATTAGEALNASVYEKLYEL
                     TGIKLHEGYGQSETTVCVYTTPWIEPKPGSMGLPGPHYDVDIQMPDGRSAEVGEQGEI
                     VIKLHKNYPVGLFKGYYRNEELTREAFRGNVYHTGDMAWKDEEGYFWFVGRTDDVIKS
                     SGYRIGPFEVESALMTHPAVVECAITGVPDELRGQVVKATVILAKEYKDKAGEELVKE
                     LQNHVKKVTAPYKYPRIIEFVDELPKTISGKIRRVEIREKDKQ"
     gene            complement(9928..10476)
                     /locus_tag="PLD44_00385"
     CDS             complement(9928..10476)
                     /locus_tag="PLD44_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOF97827.1"
                     /translation="MNIQIKQIAERLRALRESLELTPEQAAEKCGATTEEYQQYESGD
                     YDIPMSFICQAADSFGVATTALLVGDDPHAQVFFVTRKGTGASVERTKAYKYQALAYG
                     FRHPKAEPFEVTVEPNDNPLTLNTHFNQEFNYVLEGTLLLRIAGNDLILNEGDSIYFD
                     ATRPHGMKALNGKRVKFLAVII"
     gene            complement(10522..11322)
                     /gene="proC"
                     /locus_tag="PLD44_00390"
     CDS             complement(10522..11322)
                     /gene="proC"
                     /locus_tag="PLD44_00390"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOF97828.1"
                     /translation="MEIKNLKIAIIGAGNMGGALARGWSKSDILSAENIAISDISQEK
                     LDQLTAFNPHFTVSTSNREIIRQADIVLLAIKPWLVEAVAKEISKYIDYKKQVIISIA
                     AGVSFETLTHFFDSNGVFFRVIPNVAVEIGQGVSVVSSFNACKEHQQLLLEIFNVLGK
                     TFFVSENQMDAFMALTSCGIAYAFRYVRASVAGAVEMGISPQMAQEAVIQTVRGAVDL
                     LELHQSHPETEIDKVTTPGGFTIKGLNEMEANGFSNAVIKGLKASYSK"
     gene            11636..11890
                     /locus_tag="PLD44_00395"
     CDS             11636..11890
                     /locus_tag="PLD44_00395"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOF97829.1"
                     /translation="MKTNVGSTDKVVRLALAIVLIVLFYKGIFSVILGIVALIVAFVL
                     TLTSLIGFCPLYTLFGINTAKKKEQSENAEQPTKKRQPKN"
     gene            12118..13119
                     /gene="gap"
                     /locus_tag="PLD44_00400"
     CDS             12118..13119
                     /gene="gap"
                     /locus_tag="PLD44_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOF97830.1"
                     /translation="MIKVGINGFGRIGRLVFRAAQERNDIEVVAVNDPFLDKDYLIYM
                     LKFDSVHGKFQGTAENKDGKLIINGKEVLFFAEKDPANIGWGAAGAEYVVESTGVFTT
                     VEKASAHLKAGAKKVVISAPSNDAPMFVCGVNLDAYTKEMDIVSNASCTTNCLAPLAK
                     VIHDNFGIVEGLMTTIHAATNTQKTVDAPSNKDWRGGRSILNNIIPSSTGAAKAVGKV
                     IPSLNGKLTGMSMRVPTADVSVVDLTVRLEKAATYEEIKAAVKAASENEMKGILGYTE
                     DAVVSTDFIHDPRTSIFDANAGIALNSNFVKLISWYDNEWGYSNKVLDLICHMDSVK"
     gene            complement(13267..13797)
                     /gene="rplQ"
                     /locus_tag="PLD44_00405"
     CDS             complement(13267..13797)
                     /gene="rplQ"
                     /locus_tag="PLD44_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOF97831.1"
                     /translation="MRHNKKFNHLSRTSSHRKALLSNMASSLILHKRISTTLPKAKAL
                     RVYVEPLLTRAKEDTTQSRRLVFSYLQNKEAVTELFQNISVKIANRPGGYTRILRTGF
                     RLGDNAEMCIIELVDYNENMLKDKTTKKTTRTRRSSSRKKAETEVQAAPATSEVSAPK
                     PVEQAPAENQAPTTEE"
     gene            complement(13803..14795)
                     /locus_tag="PLD44_00410"
     CDS             complement(13803..14795)
                     /locus_tag="PLD44_00410"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOF97832.1"
                     /translation="MSILAFQKPEKVIMLEADDFHGKFEFRPLEPGYGITIGNALRRI
                     LLSSLEGFAITSIKIEGIDHEYSTIPGVIDDVTNIILNLKKVRFKQVVEGVDNEKVSI
                     TVSDTAVFKAGDIGKHLTGFEVLNPELIICELDPKGSFQIDIAINKGRGYVPAEENRT
                     SNLEIGVIPIDSIFTPIRNVKYAIENYRVEQVTDYEKLVIEILTDGSIPPKEALKEAA
                     KILIQHFMLFSDEKIEVETVEGETTSEFDEEVLHMRQLLKTKLTDLELSVRALNCLKA
                     ADIETLGQLCSFQRNDLLKFRNFGKKSLAELEEKLISLNLSFGMDVSKYKLDKD"
     gene            complement(14807..15412)
                     /gene="rpsD"
                     /locus_tag="PLD44_00415"
     CDS             complement(14807..15412)
                     /gene="rpsD"
                     /locus_tag="PLD44_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOF97833.1"
                     /translation="MARYIGPKTKIARKFGEAIFGPDKVLSKKNYPPGQHGNNRRKKT
                     SEYGIQLREKQKAKYTYGVLEKQFHIIYERARRASGVTGVILLQMLESRLDNLVFRMG
                     IAPTRAAARQLVNHKHITVDGKVVNIPSYQVKPGQVVGVREKDKSMEVIQNSLSGFNH
                     SKYPWIEWNASTLSGTYLHIPEREDIPENIKEHLIVELYSK"
     gene            complement(15575..15964)
                     /gene="rpsK"
                     /locus_tag="PLD44_00420"
     CDS             complement(15575..15964)
                     /gene="rpsK"
                     /locus_tag="PLD44_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOF97834.1"
                     /translation="MAKKTVAAKKRVVKVDAAGQLHVHSSFNNIIISLTNENGEVISW
                     SSAGKMGFRGSKKNTPYAAQMAAQDCAKIAFDLGLRKVKAYVKGPGNGRESAIRTVNG
                     IGIEITEIVDVTPLPHNGCRPPKRRRV"
     gene            complement(16013..16393)
                     /gene="rpsM"
                     /locus_tag="PLD44_00425"
     CDS             complement(16013..16393)
                     /gene="rpsM"
                     /locus_tag="PLD44_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOF97835.1"
                     /translation="MAIRIVGVDLPQNKRGEIALTYIFGIGRSSANKILSKAGVDRDI
                     KVKDWTDEQAAKIREIISSEYKVEGDLRSEVQLNIKRLMDIGCYRGIRHRLGLPVRGQ
                     STKNNARTRKGKKKTVANKKKATK"
     gene            complement(16420..16536)
                     /gene="ykgO"
                     /locus_tag="PLD44_00430"
     CDS             complement(16420..16536)
                     /gene="ykgO"
                     /locus_tag="PLD44_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOF97836.1"
                     /translation="MKVRASIKKRSEDCKIVRRKGRLYVINKKNPRFKQRQG"
     gene            complement(16553..16771)
                     /gene="infA"
                     /locus_tag="PLD44_00435"
     CDS             complement(16553..16771)
                     /gene="infA"
                     /locus_tag="PLD44_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOF97837.1"
                     /translation="MAKQTAIEQDGTIIEALSNAMFRVELENGHIITAHISGKMRMNY
                     IKILPGDKVKVEISPYDLTKGRITFRYK"
     gene            complement(16777..17562)
                     /gene="map"
                     /locus_tag="PLD44_00440"
     CDS             complement(16777..17562)
                     /gene="map"
                     /locus_tag="PLD44_00440"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOF97838.1"
                     /translation="MIYLKSEEEIELLRISNQLVAKTLAELAKHITPGISTLELDRIA
                     DEFIRDHGAVPSFLDYNGYPNSICTSVNEQIVHGIPSDKVFLKEGDIISIDCGAYING
                     FHGDSAYTFCVGEVDQQIVNLLKTTKESLYKGIEQAQEGHRTGDIGYAVQSYCEQRGY
                     SVVREMVGHGLGREIHEAPEVPNYGRKGNGVMLKYGMVICIEPMINMGTRHIVFEKDG
                     WTTRTLDRKPSAHFEHAVAIRQGKADILSSFEYIEQVLGERAI"
     gene            complement(17580..18923)
                     /gene="secY"
                     /locus_tag="PLD44_00445"
     CDS             complement(17580..18923)
                     /gene="secY"
                     /locus_tag="PLD44_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOF97839.1"
                     /translation="MNKLIETLKNIWKIEDLRNRLLTTLLFVMIYRFGSYVVLPGIDA
                     GALTALKAQTSGGLLALLDMFSGGAFANASIFALGIMPYISASIVIQLLTIAVPYFQK
                     MQREGESGRRKMNQITRYLTVGILLLQGPSYLINLSVQLRQAGSALPSGFFFQATSTI
                     ILCAGSMFVMWLGERITDRGIGNGISFIILVGIIARLPQAIVREFASRLTDVGGGLIM
                     FIIEILFLLLVIGASIMLVQGTRKVPVQYAKRVVGNKQYGGVRQYIPLKINAAGVMPI
                     IFAQAIMFIPITIVGFSKSESMQGFVGAFMDNTGFWYNFVFAILIILFTYFYTAITIN
                     PTQMAEEMKRNNGFIPGVKPGKKTAEYLDMIMSRITLPGSIFLAIIAIMPAFASIFGV
                     NHAFAQFFGGTSLLILVGVTLDTLQQIESHLLMRHYDGLLKSGRIKGRTSGVSAY"
     gene            complement(18925..19374)
                     /gene="rplO"
                     /locus_tag="PLD44_00450"
     CDS             complement(18925..19374)
                     /gene="rplO"
                     /locus_tag="PLD44_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOF97840.1"
                     /translation="MDLHNLTPAAGSVKSNKRKGRGSGSGMGGTSTRGHKGAKSRSGY
                     AHKIGFEGGQMPIQRRLPKFGFKNINKIEYKAINIDTLEILAQENQLSKIGIDDLRQA
                     GFISKNQLVKILGRGKLTVKLDVEAHAFSKSAENAIVGAGGTAVKIS"
     gene            complement(19404..19580)
                     /gene="rpmD"
                     /locus_tag="PLD44_00455"
     CDS             complement(19404..19580)
                     /gene="rpmD"
                     /locus_tag="PLD44_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOF97841.1"
                     /translation="MATIKIKQVKSKIKSPKVQKLTLEALGLHKINDVVEHEATPQIL
                     GMVEKVKHLVKIVD"
     gene            complement(19593..20111)
                     /gene="rpsE"
                     /locus_tag="PLD44_00460"
     CDS             complement(19593..20111)
                     /gene="rpsE"
                     /locus_tag="PLD44_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOF97842.1"
                     /translation="MAKMMNRVKTTTDLELKDRLVAVNRVTKVTKGGRTFSFSAIVVV
                     GNEDGIVGWGLGKASEVTAAIAKGTEVAKKNLIKVPVLNGTIPHEQEAKFGGARVFIK
                     PASSGTGVTAGGAMRAVLESVGITDVLAKSKGSSNPHNLVKATIAALSELRDAYTVAQ
                     NRKVSLDKVFNG"
     gene            complement(20118..20459)
                     /gene="rplR"
                     /locus_tag="PLD44_00465"
     CDS             complement(20118..20459)
                     /gene="rplR"
                     /locus_tag="PLD44_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOF97843.1"
                     /translation="MTKLDRRQKIKAHIRKKIFGTAQRPRMTVFRSNAQIYVQLIDDE
                     KGITLASASSLAIKEKITKQEQAGKVGALIAQAAKEAGITEVVFDRNGYLYHGRVKQL
                     ADAAREGGLKF"
     gene            complement(20481..21032)
                     /gene="rplF"
                     /locus_tag="PLD44_00470"
     CDS             complement(20481..21032)
                     /gene="rplF"
                     /locus_tag="PLD44_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOF97844.1"
                     /translation="MSRIGKLPIKIPAGVNVTIKNNLVSVSGPKGTLSQQIHPNISVE
                     VKDGFCTLTRIDDEKENRAMHGLYRSLINNMVEGVSNGFKKTMELVGVGYRVSAQGQV
                     LEFALGYSHSIFLKVPPEVKVETKSERNQNPLIILESCDKQLLGQVCAKIRSFRKPEP
                     YKGKGIKFQGEVLRRKAGKSAGK"
     gene            complement(21051..21446)
                     /gene="rpsH"
                     /locus_tag="PLD44_00475"
     CDS             complement(21051..21446)
                     /gene="rpsH"
                     /locus_tag="PLD44_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOF97845.1"
                     /translation="MTDPIADYLTRLRNAIKANHRVVEIPASNLKREITRILFEQGYI
                     LNYKVVEDGPQGTIKIALKYDPVNKVNSIKKLIRVSKPGLRKYVGYQDIPRVLNGLGI
                     AILSTSSGVMTNKEAAKLKIGGEVLCYVY"
     gene            complement(21567..21836)
                     /gene="rpsN"
                     /locus_tag="PLD44_00480"
     CDS             complement(21567..21836)
                     /gene="rpsN"
                     /locus_tag="PLD44_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOF97846.1"
                     /translation="MAKESMKAREVKRNKMVAKYEAKRSLLLAEGNYEALQSIPKNAS
                     PVRLHNRCQLTGRPKGYIRQFGVSRIVFREMASKGLIPGVKKASW"
     gene            complement(21850..22395)
                     /gene="rplE"
                     /locus_tag="PLD44_00485"
     CDS             complement(21850..22395)
                     /gene="rplE"
                     /locus_tag="PLD44_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOF97847.1"
                     /translation="MNTASLKQEYKERIVPALMKEFAYKSVMQVPRLEKIVLNQGLGI
                     AVADRKIIDTAINEMTTITGQKAVATVSKKDISNFKVRKKMPIGVRVTLRGDRMYEFL
                     ERLIRVALPRIRDFKGIENKLDGRGNYTLGITEQIIFPEINIDSISRLLGMNITFVTT
                     AKTDEEGFALLKEFGLPFKKN"
     gene            complement(22398..22709)
                     /gene="rplX"
                     /locus_tag="PLD44_00490"
     CDS             complement(22398..22709)
                     /gene="rplX"
                     /locus_tag="PLD44_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOF97848.1"
                     /translation="MTKLHIKKGDIVYVNTGVDKGKTGRVLEVLVKEQRAIVEGINLV
                     SKNMKPNAKYPQGGIVKQEAPIHISNLNPVENGRPVRVGRKLNNEGKLVRISKKTGEE
                     I"
     gene            complement(22732..23097)
                     /gene="rplN"
                     /locus_tag="PLD44_00495"
     CDS             complement(22732..23097)
                     /gene="rplN"
                     /locus_tag="PLD44_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOF97849.1"
                     /translation="MIQQETRLLVADNSGAKEALCIRVLGGTGKRYATVGDTIVVSIK
                     SVIPSSDIKKGMVSKAVVVRTKKEVRRVDGSYIRFDDNACVLLNNAGELRGSRIFGPV
                     ARELRATNMKIISLAPEVL"
     gene            complement(23100..23357)
                     /gene="rpsQ"
                     /locus_tag="PLD44_00500"
     CDS             complement(23100..23357)
                     /gene="rpsQ"
                     /locus_tag="PLD44_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOF97850.1"
                     /translation="METRNLRKERIGVVTSNKMDKTITVAVRWKEKHPIYGKFVNKTK
                     KYYAHDEKNECNIGDTVSIMETRPLSKLKRWRLTEIIERAK"
     gene            complement(23372..23566)
                     /gene="rpmC"
                     /locus_tag="PLD44_00505"
     CDS             complement(23372..23566)
                     /gene="rpmC"
                     /locus_tag="PLD44_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOF97851.1"
                     /translation="MKTREIRELSTKELLERLETEKENLVRLKLNHSISPLDNPMQIK
                     VVRRNIARIATELRLREINQ"
     gene            complement(23570..24004)
                     /gene="rplP"
                     /locus_tag="PLD44_00510"
     CDS             complement(23570..24004)
                     /gene="rplP"
                     /locus_tag="PLD44_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007365583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOF97852.1"
                     /translation="MLQPKKTKYRRQQKGRMKGNAQRGNQLAFGSFGIKSMDTVWLTG
                     QQIEAARIAVTRYMQRQGQVWIRVFPDKPITKKPAEVRMGKGKGSPEGFVAPITPGRI
                     IIEVDGVPYAVAKEALRLGAQKLPIITKFVVRRDYDLTDQNL"
     gene            complement(24029..24781)
                     /gene="rpsC"
                     /locus_tag="PLD44_00515"
     CDS             complement(24029..24781)
                     /gene="rpsC"
                     /locus_tag="PLD44_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOF97853.1"
                     /translation="MGQKTNPISNRLGIIRGWDSNWYGGKKYGDTLLEDSKIRKYLNV
                     RLSKASVSRIIIERTLKLVTITVCTARPGIIIGKGGQEVDKLKEELKKITDKEIQINI
                     FEIKRPELDAVIVASNIAHQLEGKIAYRRAVKMAIASTMRMGAEGIKVQVSGRLNGAE
                     MARSEMYKEGRTPLHTFRADIDYACAEALTKVGLIGIKVWICRGEVYGKRDLAPSYVT
                     GQKEGSRTERRSGDHHSGGDHGNKNSSRRKKK"
     gene            complement(24790..25203)
                     /gene="rplV"
                     /locus_tag="PLD44_00520"
     CDS             complement(24790..25203)
                     /gene="rplV"
                     /locus_tag="PLD44_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOF97854.1"
                     /translation="MGARKKISADRRKEARQTLYFAKLNDVPTSPRKMRLVADMIRGM
                     EVNKALSVLKFSSKEASRRMEKLLKSAISNWEIKTEQKADNANLFVSKVYVNEGTTLK
                     RLRTAPQGRGYRIRKRSNHVTLYVDSRNNNDTNQI"
     gene            complement(25247..25513)
                     /gene="rpsS"
                     /locus_tag="PLD44_00525"
     CDS             complement(25247..25513)
                     /gene="rpsS"
                     /locus_tag="PLD44_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOF97855.1"
                     /translation="MSRSLKKGPYISLKLEKKILDMNESGKKTVVKTWARASMISPDF
                     VGHTIAVHNGNKFIPVYITENMVGHKLGEFALTRTFRGHAGKKK"
     gene            complement(25536..26360)
                     /gene="rplB"
                     /locus_tag="PLD44_00530"
     CDS             complement(25536..26360)
                     /gene="rplB"
                     /locus_tag="PLD44_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOF97856.1"
                     /translation="MAVRKLLPTTPGQRNKIISKFDTITKREPEKSLVYGKRKTGGRN
                     NNGKMTMRYIGGGHKKKFRVIDFTRKKDGIPATVKAIEYDPNRSARIALLYYADGEKS
                     YILAPNGLQVGQKIISGSNVAPEVGNALPLENIPLGTIVHNIELRPGQGAAIARSAGT
                     FAQLVSREDKYVIIKLPSGEVRKILGACKATVGSVGNSDHALEKSGKAGRSRWLGRRP
                     RVRGVVMNPVDHPMGGGEGRASGGHPRSRKGLLAKGYKTRHPKKQSSKYIIERRKK"
     gene            complement(26370..26660)
                     /gene="rplW"
                     /locus_tag="PLD44_00535"
     CDS             complement(26370..26660)
                     /gene="rplW"
                     /locus_tag="PLD44_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOF97857.1"
                     /translation="MGVILKPLVTEKLTKLGDKFNRYGFKVQKDANKLQIKQAVETMY
                     NVTVVDVNTMIVPSKKRSRYTKSGVISGKRPAYKKAIVTVKKGEEIDFFSNI"
     gene            complement(26675..27307)
                     /gene="rplD"
                     /locus_tag="PLD44_00540"
     CDS             complement(26675..27307)
                     /gene="rplD"
                     /locus_tag="PLD44_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOF97858.1"
                     /translation="MELSVLNIKGEDTGRKVSLNDAIFGIEPNDHAIYLDVKQFLANR
                     RQGTHKAKARSEISGSTRKLGRQKGGGGARPGDIKSPLRVGGGRVFGPVPRDYSFKLN
                     KKVKQLARKSALSYKAKNGAITVVENFEFEAPKTKKMLELAKNLQVADKKPLIVLSSA
                     NKNVYLSARNLPSINVITTSELNTYNVLNAKTLVLTEGSVTILEQIFKVE"
     gene            complement(27310..27927)
                     /gene="rplC"
                     /locus_tag="PLD44_00545"
     CDS             complement(27310..27927)
                     /gene="rplC"
                     /locus_tag="PLD44_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOF97859.1"
                     /translation="MPGLIGKKIGMTSVFSADGKNVPCTVIEAGPCVVTQIKSIETDG
                     YEAVQVGFEEKKPKHTNKPEAGHFKVAGVAPQRHLVEFKGFEGDFKLGDVITVEIFDG
                     STFVDVVGTSKGKGYQGVVKRHKFSGVGETTHGQHNRQRAPGSLGASSFPSRVFKGMR
                     MGGRMGGDKVTVQNLQVLKIIPEHNLLLVKGSVPGAKGSIVIINK"
     gene            complement(28047..28352)
                     /gene="rpsJ"
                     /locus_tag="PLD44_00550"
     CDS             complement(28047..28352)
                     /gene="rpsJ"
                     /locus_tag="PLD44_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOF97860.1"
                     /translation="MSQKIRIKLKSYDYNLVDKSAEKIVKTVKATGAVVSGPIPLPTH
                     KRIFTVNRSTFVNKKSREQFELSSYKRLIDIYSSTSKTVDALMKLELPSGVEVEIKV"
     gene            complement(28422..30545)
                     /gene="fusA"
                     /locus_tag="PLD44_00555"
     CDS             complement(28422..30545)
                     /gene="fusA"
                     /locus_tag="PLD44_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOF97861.1"
                     /translation="MAKVDLSYTRNIGIMAHIDAGKTTTSERILFYTGLTHKIGEVHD
                     GAATMDWMEQEQERGITITSAATTTYWNYNQHQYKINLIDTPGHVDFTVEVERSLRIL
                     DGAVATFCAVGGVEPQSETVWRQADKYNVPRLCFVNKMDRSGADFFEVVRQIREVLGA
                     KPCPIQIPIGAEEKFKGVVDLIKMKAIFWHDETMGAEYSEEEIPADLVNDAREWREKM
                     LEIVAEYDDAVMEKFFDDPSKITEDEIKAAIRKGTLAKEINPVICGSAFKNKGIQTML
                     DAVCAYLPSPLDNPEIVGKDPRYEDKTVVRHPDPNEPLCALAFKIATDPYVGRLCYFR
                     VYSGKLESGSYVYNPRTDKKERISRIFQMHSNKQNPVDMISAGDIGAGVGFKDIKTGD
                     TLCDENHPIVLESMDFPEPVIGISIEPKTQKDLDKLGVGLSKLAEEDPTFTVHTDEQS
                     GQTIISGMGELHLEIIIDRLRREFKVECNQGRPQVAYKEAITQPVEIREVYKKQTGGR
                     GKFADIIVRVEPADNDFEGSLQFIDMVKGGNIPKEYIPSIQKGFQSAMKNGVLAGFPV
                     DRLKVTVLDGSFHPVDSDQLSFEICAQIAFKNACTKAKPVLLEPIMKIEVVTPEESMG
                     DVIADLNKRRGQVEGMESSRTGARIIKAKVPLAEMFGYVTALRTITSGRATSSMEFSH
                     YAEVSNSLAREVVAEAKGKVELLHY"
     gene            complement(30565..31041)
                     /gene="rpsG"
                     /locus_tag="PLD44_00560"
     CDS             complement(30565..31041)
                     /gene="rpsG"
                     /locus_tag="PLD44_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOF97862.1"
                     /translation="MRKSKPKRRVVLPDPIFNDPMVTKFVNHLMFDGKKSIAFDIFYS
                     ALENVKNKLPNEEKSSLEIWKKALENVTPLVEVRSRRVGGATFQVPTEIRPERKESIS
                     MKNLILYARKRGGHSMSDKLAAEIVDAFNSQGGAYKRKEDIHRMAEANRAFAHFKF"
     gene            complement(31233..31643)
                     /gene="rpsL"
                     /locus_tag="PLD44_00565"
     CDS             complement(31233..31643)
                     /gene="rpsL"
                     /locus_tag="PLD44_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOF97863.1"
                     /translation="MPTIQQLVRKGRTELVDKSKSPALDSCPQRRGVCIRVYTTTPKK
                     PNSALRKVARVRLTNQKEVNAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGTL
                     DTAGVNGRLQRRSKYGAKRPKKGQPAAASTGKKK"
     gene            complement(31947..33740)
                     /gene="argS"
                     /locus_tag="PLD44_00570"
     CDS             complement(31947..33740)
                     /gene="argS"
                     /locus_tag="PLD44_00570"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOF97864.1"
                     /translation="MNLEHTIQQLVEQALQQLYGLEIGQNPLQVQKTRKEFEGDMTVV
                     LFPFIKLLRKSPEQIGKEIGDNLLKSSSVIEDYNVVKGYLNLKISVSYWLERLNEIFL
                     QKDFWKVKLKENADLMMIEYSSPNTNKPLHLGHVRNNLLGWSIAQIQEANGWKVVKTN
                     LVNDRGIHICKSMLAWKLFGNGETPQSSGLKGDHLVGKYYVLFDQAYKQQVRELVEEG
                     WSEEEAKKSAPLMQEAQKMLLAWENNDPEVRKLWETMNGWVYEGFNETYRKLGIDFDK
                     IYYESETYLVGKKIVEEGLGKGIFYRRPDGSVWADLRDDGLDEKLLLRADGTSVYMTQ
                     DIGTAKVRYDDFPIKKMVYVVGNEQNYHFQVLSILLDKLGFEWGKSLVHFSYGMVELP
                     NGKMKSREGTVVDADDLIDEMLRTARETSEELGKLDGCSPEEVENIVRMVALGALKYF
                     ILKVDPRKNMTFNPKESIDFNGNTGPFIQYTHARIRSVLRKAQEQSISFGKLNNLNLS
                     ISQKERYLVQLLTEFPLMVKEAGDEFNPALIANFLYELVKEYNQFYHDYSILKEENTD
                     LRNFRLVLSSAIADVIKDGMALLGIEVPERM"
     gene            complement(34001..34400)
                     /gene="ssrA"
                     /locus_tag="PLD44_00575"
     tmRNA           complement(34001..34400)
                     /gene="ssrA"
                     /locus_tag="PLD44_00575"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(34417..35937)
                     /gene="rny"
                     /locus_tag="PLD44_00580"
     CDS             complement(34417..35937)
                     /gene="rny"
                     /locus_tag="PLD44_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOF97865.1"
                     /translation="MNLIIAIIISFGVGVGGSFIYFKRNHKKALKELEAEAELIKKNN
                     LIEVKEKFIALKAEHEQQVQQRNAKIQAAETRIQQREMQLNQKQGELQRKMNEVDVMK
                     ENLESQFLTVENKKKELEHLIRQAQEQLETVSGLSSEQAKERLIESLKEEAKTEAMAY
                     VNDIMDEAKMTANKEAKRIVIQSIQRLATETAIENSVTVFHIESDEIKGRIIGREGRN
                     IRALEAATGVEIIVDDTPEAIVLSAFDPVRREIARLSLHQLVTDGRIHPARIEEVVNK
                     VRKQLEEEIIEVGKRTTIDLGIHGLHPELIRLVGKMKYRSSYGQNLLQHSRETANLCA
                     TMAAELGLNVKKAKRAGLLHDIGKVPDDEPELPHAILGMKLAEKYKEKPDICNAIGAH
                     HDEIEMESLIAPIVQVCDAISGARPGARREIVEAYIKRLKDLEDLALSYPGVLKTYAI
                     QAGRELRVIVGADKIDDKEAELLSFDIAKRIQDEMTYPGQVKITVIRETRAVSYAK"
     gene            complement(36091..36387)
                     /locus_tag="PLD44_00585"
     CDS             complement(36091..36387)
                     /locus_tag="PLD44_00585"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOF97866.1"
                     /translation="MNENDTFLINVQIGGYRLPLRIERKEEELYRLAEKVLGKYLEVY
                     QKKYSHRSAEEVFIFVAYELAVMLVRKMMQQDTAPLAEKVSSLNQELEKLLAEK"
     gene            complement(36395..36691)
                     /locus_tag="PLD44_00590"
     CDS             complement(36395..36691)
                     /locus_tag="PLD44_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97867.1"
                     /translation="MTHSYDDLVATFESNLRKLMEDFKSLQEENSLLRAQLSNKEQDI
                     ELLKVKFSELQKNYDHLRIAKTLSLSDEDRIKSKQQINKLVREIDKCLALLLND"
     gene            36894..38678
                     /gene="ispG"
                     /locus_tag="PLD44_00595"
     CDS             36894..38678
                     /gene="ispG"
                     /locus_tag="PLD44_00595"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOF97868.1"
                     /translation="MDPFNYQRRPSTEVRIGQLLMGGTQPIRIQSMTNTDTNNIEASL
                     EQCIRLIRAGSELVRFTTQGNREAESLKKIHDLLRDKGYQTPLSADVHFNANVADVVA
                     QYVEKVRINPGNYLGSNRGNITSDYSDEEYKAEYERIRKRFKQLLNICNKHQTALRIG
                     VNHGSLSERIMNRYGDTAEGMVESCMEFLRICREENFHNVVISMKASNTLTMIHSVRM
                     LVERMEAENFHFPLHLGVTEAGNEEEGRIKSAVGIGTLLSEGIGDTIRVSLSEDPEAE
                     IPVARKLISYIDAKKNHEPIIAEKNFQHSPFNYHRRTTHAVENIGGNNVPVVIAHTKE
                     NFEITPDYYFAENQIINIHNEVFPIFDIDEKEKIIKHSSRIKFLKMSFPELTKENVHF
                     LKNTADTVVILESHHKNAPAEQKAFFHSLMNAGCTVPVIVTQSYKENIEEDLEIKAGV
                     DFGMLFVEGFGDGIFLKNEGTIPSEKLNSIAFGILQATRARISKTEFISCPGCGRTMF
                     DLQTVVSRIKSKTSHLKGLKIGIMGCIVNGIGEMADADYGYVGAGRGKVSLYKKKICV
                     EKNIPEEEAVEKLIELIKINGDWTEPIS"
     gene            38732..39196
                     /locus_tag="PLD44_00600"
     CDS             38732..39196
                     /locus_tag="PLD44_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97869.1"
                     /translation="MKEKLIVLLLASASFMGLNAQVRGQALGVRLGYGGEVSYQHPLN
                     SQNRIEGDLGFYYDHGFNLAGVYQWVFDLSELSPGFNWYVGLGPQIGSWSKHNNKDYD
                     GLALGIVGQIGIEFNFNIPLQLSLDYRPGWYVLPSSYGGAWEGIALGVRYNF"
     gene            complement(39327..39746)
                     /gene="secG"
                     /locus_tag="PLD44_00605"
     CDS             complement(39327..39746)
                     /gene="secG"
                     /locus_tag="PLD44_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOF97870.1"
                     /translation="MYLLFTILIIIASILLILIVLVQNSKGGGLAAGFSSSNQYMGVR
                     KTADFLEKATWGLIAFIVVLSIGATGIHANQKSGGSDSEIKEQYQKAQEELPGVVAPD
                     FGETQGAGNNQQDNNGGQQNNGNQQKAPQKLPVVPAK"
     gene            complement(39760..40416)
                     /locus_tag="PLD44_00610"
     CDS             complement(39760..40416)
                     /locus_tag="PLD44_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97871.1"
                     /translation="MTQAEFLTLLQHPESITSEHTPDLKDLVEHYPYFSQARMLYLKA
                     LQVSGSIHFEKTIKTAVVYTSQRRWLYHFLYPEVFAKEVYRRPEKGKLTGSYFDLLDV
                     VEKEGGDSGQTLKNLADQLKKARAMVMENPPAPDEEEPIKKKGSTKSSALQSEYFAEE
                     IIPELEISEESVKRLIKNRKYTEALEILKKLNLNNPKKSVYFADQIRFLEKVIENLKI
                     "
     gene            complement(40456..41016)
                     /locus_tag="PLD44_00615"
     CDS             complement(40456..41016)
                     /locus_tag="PLD44_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOF97872.1"
                     /translation="MELNRKPRNSFFGRNTKSVKLLSAIFCGTFLLLASCTVSYKFNG
                     ASIDYTKIKTISIADFPNVSELVYPPLSQQFSEALRDIYTKQTQLRLVRQNGDLNLEG
                     EIVGYQLTPMSISADTYASETKLTVTVKVRFTNKVNPADDFEKTYSAFQVFDSSKMLN
                     DVQEELVKAIITEITENIYNDTVAKW"
     gene            complement(41003..42298)
                     /locus_tag="PLD44_00620"
     CDS             complement(41003..42298)
                     /locus_tag="PLD44_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOF97873.1"
                     /translation="MQPSEIQAVKQRFGIIGNSELLNRAIDVAVQVAPTDLSVLITGE
                     SGVGKENFPQIIHYYSHRKHGPYIAVNCGAIPEGTIDSELFGHEKGSFTGAMADRKGY
                     FEVADGGTIFLDEVGELPLSTQVRLLRVLETGEFIKVGSSKIQKTNVRVIAATNLNMT
                     QAVRDGRFREDLYYRLNTVPIYVPPLRERKEDIVLLFRKFAADFAEKYHMPPIKLTED
                     AKKLLTEYYWNGNVRQLKNITEQISVIEQQREITAEILKQYLPKDELERFPVLLSNHR
                     SDQKTFTSEREILYQVLFDMKQDMIELKKLVYNLMNAQGNQELLQKDFPLSSSYVAPH
                     ETFVPATPVPVDVMKPVNNLGREVNQFPNTNKQNSFEEAAEFQEIEDIQEPMTLEDME
                     RQMIKKTLEKHKGKRKAAAEELKISERTLYRKIKEYGIE"
     gene            42637..43380
                     /locus_tag="PLD44_00625"
     CDS             42637..43380
                     /locus_tag="PLD44_00625"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOF97874.1"
                     /translation="MMEDNFIRLMKAQQKSPADEVINKIKELIESGELKPGDRLPAER
                     KLASDFGFGRTHIREALQKLEFYGIVKTLPQSGTILNGLDINSLNGLLSDVLNLQSYD
                     FYSLVETRCILEMNAIRLCAERRTEEDIEAMEKSHQNFIKFHNDEILRVSYDFAFHRN
                     IAEGSHNPVIKSMLMTIIPDILIIYNKERVCNTDPFVVIKEHAEMINAVKMQNGELAS
                     SLLYQHVQGVMTLARTKLENLKVKSLSMA"
     gene            43692..46931
                     /locus_tag="PLD44_00630"
     CDS             43692..46931
                     /locus_tag="PLD44_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOF97875.1"
                     /translation="MKRTKKPKFLKREVLVLIALFISTISAFSQIKVTGTVLDTKREP
                     LIGVNITVKGSRTGTISDFNGKFNIDVPDRQSVLVFSYIGYKTIERTIPASGQLTVIL
                     EEETQTIDEVVVVAYGTQKKSHLTGAVSSLKNEKMDEIPVSRVDQALQGKIAGVQINN
                     VNPEAGAAPKIRVRGLGSISASSDPLIVIDGFPVPDGLSMVSMADVQSVEVLKDASSA
                     ALYGSRAAGGVILITTKSGEINKPKYNFKMYSGFRNPLKLPDMLSTEEYVALLYREAD
                     MRKLDPAVNGIATETMAFNKITEPEKAGYLIMKYLDDQPTDWLNEGLRDRGTMQNYQL
                     SASGGDKNLKYFVSGNYVNEDGIMLNSTYDKFTFRAKLDAKLSKAATLGVNLSPTYSR
                     QERPAVDLTDYMRFPSWLPIRHNEATAALTGKVAGDYAQPSDFNGINISGIGYNNEVW
                     SLPGVTVWNSSNQNPVSIRERTSIFTDDYRFQGNIYLTLNLLPGLQFKTSNGAYLLFR
                     EYNKKEQTSANKAGNPNSLTRQLTTHRELLSENTLNYNKKISNHEFDALLGFTMQQTN
                     DNYNRIVGTNFPDEQILSFNLASQILLDSPSIDGTTSFYYTESLVSLLGRLNYAYLGK
                     YMMSASFRADGSSKFYKGNKWGTFPAFSAGWRASEEKFMKNFEWLSNLKLRVSYGLTG
                     NNNIPQYAYMNSINTSNYVLGSGNGTLVQGMVSNSSSLGNPDITWEQTSEANYGIDFG
                     FLNSRLNFSIEYYNSNTIQLLLQQPAMYITGHQSYWNNIGKVNNKGIEIELNTTNIAN
                     KKFTWKTSANFSTNKNKLLNYGDKEYEDNFGERSEVYRAIVGEPAIQYYGYKTDGVYT
                     TFEEVAAAKALKDKNGNPFTYTKFAPIVGGIKVVNINGDNKIDTEDRTVLGDPFPDFT
                     WGITNTFTYKNFDLSFLIQGVQGIDIINGNVNYNEQLRYNKAYTNNRYVSPMFPGDGK
                     TVYSNTTSGSDLMLTDYCIEDGSYGAIRDFTLGYTLPQNLLKSLKLRNLRVYFSAENL
                     VYIMAPSYRGINPEARRTASNYTSPLVDGYQRGAFPLNRTFTAGLDINF"
     gene            46976..48514
                     /locus_tag="PLD44_00635"
     CDS             46976..48514
                     /locus_tag="PLD44_00635"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOF97876.1"
                     /translation="MKNKIFITAISLCGFFLTSCEDFLTDKPESVLTQVDFYTTPIRI
                     NQGVIGCYAGLANIQKDEWMYTELRSDNSCQSETGSSATIRIDQTDIASFRLSPSLPA
                     LRDYWYKIFQNISNINAVLPSVRDNSYVANESLRAQYEGELLFLRAYHYYTLVNLFGD
                     MFKITKVIGPNEAKKIPRSPVSEIYNEIIIPDLKKAVEELPSTYSATDLGRATKWAAK
                     GMLAKAYMMLGGKDNLSLAKKELEDILTLSPHGLLTGTGAYANIFSISNEMNKEIIFA
                     VRYKGGSSGIGSPFWGTFAPLGSANLFLKIGTPLGYNNPTYELMNLFNANPNDTRKDA
                     CFKVWNKSSTVSVPYISKYMDASMTQPLQSENDWIILRFADIKLLYAEILAQDGNFAN
                     AHNPLNDVRARAGLDGIGPFGSKEEALDAVYAERRLELAFENQRWFDLLRMAKSYDNP
                     DKPMEILRRHTFETDWAVLYSKYNPMQPPEERFYIKERLLLPIPQAEIDTNNEMVIPQ
                     NPTY"
     gene            48542..49141
                     /locus_tag="PLD44_00640"
     CDS             48542..49141
                     /locus_tag="PLD44_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97877.1"
                     /translation="MKNIKKFSLFFLFIGLIGASCSTDEENTVVPKTLEQYKQDFSQF
                     VSAEKTKVQNCKMGYNKGDFKYSSDSTNFKTYCADYLNVLIAAEAVLAKSDLTIADIV
                     KANGTLASPGKLFNSSLWISDRRPLNDAIIAAQELLAATPEGTDPGQAPAEARTAFTN
                     AITAAKKVRDSSYTIERQVQEAVQKLEEAKNAFLAAIIH"
     gene            49168..51555
                     /locus_tag="PLD44_00645"
     CDS             49168..51555
                     /locus_tag="PLD44_00645"
                     /inference="COORDINATES: protein motif:HMM:NF025943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 6 family protein"
                     /protein_id="HOF97878.1"
                     /translation="MKKTFGILLFMFLPFSVILGTSYKVMNLDQFNEKSKLLLPGDSI
                     ILSNGVWKDAQLVFKGNGEKNKYIYLLAENSGKVSLEGESCLRMSGNWLYVSGLVFRN
                     GHTPRKTVIEFRTGSKEYAFNSVLAECVVDQFNQKVKDSTDHWIALYGQKNTIRNCYF
                     AGKTNLGTTLIVCPDDENSINNQHLIYRNYFGFRPRLGSNGGETIRIGTSEVCTNSSE
                     TIVKGNYFEHCSGETEIISNKSTDNSFLNNTFYECEGSLVLRHGNNATVAGNWFIGNG
                     KAFTGGIRVINEGHKIFNNYFYRLAGDDFRSSLAIMNGIPNSPANGYAPVKNVIVANN
                     TYYDCTSPWSFGVGFGFRNRIAKPENTLLLNNLVYCPNTDELIKYYDQMDGIMLDNNI
                     LVNSKGNLEMKGTVKGEIFQSKMNDLEIVYTNTTAKRLAFIKEDILGQVRTTPVIGAF
                     QNNIVNPTIAMATKHNCGPEWYRNKITETTKTEKRFGKATVVEAGLDLLSKAVKNAGD
                     NDTLLLMPGEHVVTKKMAINQNLVIRSADNKVKPIIIMKADKENAYMFEISNNVKLQL
                     EGVKIDGNSESNFPTKYAFISSREGAAHFSLIVNQCEISGFNVKTGSIYRAYKGSYAD
                     SIVVTNTVMKDCFRGFSLSEEKDDKGTYNAEIVKFENSVFDNFTQHALDYYRGGNDES
                     TLGGFLFVNHCVFNNIGQDEKQSMLKLNGIVNVSIANSIFSNSMAKTSVKLSGNKNII
                     QNCCFYNCPAPKVENGAVSQNLYFDNPRFEKKSFLLSKKSPLIGKATDGGNIGLR"
     gene            51682..52770
                     /locus_tag="PLD44_00650"
     CDS             51682..52770
                     /locus_tag="PLD44_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOF97879.1"
                     /translation="MQTAIQRSEEQLSAAIVQFRDSNKTPRTFENGKIVLVSYKDWTS
                     GFFPGSLWYIYELTGNKKFEKTAEHFTTLLNKAQYRTDTHDLGFILNCSYGNAYRITK
                     NTDYKTVLINGANSLMKRYHPKVGCIKSWDFGNWQYPVIIDNMMNLEFLMNVGKMTDN
                     QPMINAAISHADKTMKNHFRSDFSCFHVVDYDTLTGKVIKKQTHQGFNDSSSWARGQS
                     WALYGFTMMFRETGKVEYLHQAEKIASFLLNHPRLPKDKIPYWDFDAPNIPFEPRDAS
                     AAAIMASALIELSTFVKDKPEYFKTATIILKNLSSDKYLSKKGKNGFFILKHSTGNYP
                     AKSEIDVPLSYADYYFLEALQRYLEYKK"
     gene            52864..53967
                     /locus_tag="PLD44_00655"
     CDS             52864..53967
                     /locus_tag="PLD44_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97880.1"
                     /translation="MKNKYLIIPTIIIFTSFTLKSQTLLEANGPGNTYELINSIFAPN
                     GGNVVETPDCAHPEFGRHIAEVWDEDLQKYVFEFYIHVTPDNDRCINFDRQRVEIKTY
                     EPSPENLKGTSGETVVYKWFFKIPTGFQPSSNFTHLHQIKPVGGDEDTPIFALTARKG
                     NPNRLELNYYESSNQSAIKLKTIYLSWLENSWVEVTERIKIDPENGNYAITIKRINDG
                     IVLLDYENQHLLTIRPDNNFIRPKWGIYRSLLSSQDLRDESVRFADFSIEEVNSTALN
                     KTILENSNFSFFNDSKNKKLKVNFTLNQPSDVAFSCMLLNGQRAKEFSSYKNLPVGEH
                     QAYLDFGNMLPGIYLLSMKTSYGVISKKMLIQP"
     gene            54181..55038
                     /locus_tag="PLD44_00660"
     CDS             54181..55038
                     /locus_tag="PLD44_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOF97881.1"
                     /translation="MSSSQEKLPVCIFSKHLQFLDWRSLGEKVTEMGFAGIDLTVRKD
                     GHVEPAAVETDLPKAVDEIKKGGSTCVMLSTTIENVHNEQDRNVIESAAKCNIPFYRT
                     NWFKYPADKSQPEALEMYASNLKEMSELNRKLGIVGCYQNHAGTLVGASLWEVYKMLE
                     KVDKDYFGVQYDIRHAMVEGANSWINGFELIRSQIKTIVLKDYKWGQVNGKWKVVNTP
                     IGEGMVDFKNYFKLLKKYRINVPISLHCEYDLGGAEKGNRKISVYPQVVFDAMTKDLK
                     TIQQLWEEA"
     gene            55077..>55536
                     /locus_tag="PLD44_00665"
     CDS             55077..>55536
                     /locus_tag="PLD44_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97882.1"
                     /translation="MNFDKLKILTSAICFFCGSFLFAQQRIPSDVVISPENLAQYLND
                     SVKNKLSKNGKITETVLTEYFRQKFAERFFYDWHSVPSRFQQYRQLYDNESGHLSRAK
                     ELMTDFSATTSWLLPFNHPNGEAVKPHPFQHLARQHKMVDVGFAYFYDQKN"
BASE COUNT        16722 a        11264 c         9957 g        17593 t
ORIGIN      
        1 tattttccaa atgtcaataa attcggtaaa agattgaata acattgatgg ataatgattt
       61 gaacttcacg acagaaatgt tgaaagctgt ctttcttttc taaaaaaatc tatttgtatt
      121 tttctacaga aacgattttt taaccttttc gtttttctga agctgctttt atgcttgaaa
      181 aatacatgcc aaaagaccga tacaggaaat gagaccattt tccaaatgga acaataatta
      241 tagcccattg agccaaaacg gttaaatgaa cgatatacat ccatacatta ttgtctatca
      301 tatgggtatc gataaataaa cgtacaagga aagctgtaag ccccataagc ataagccata
      361 taacaaacag ccagtcggaa ggctgcgaaa atttgttcag tgcctttgct ttcctaattc
      421 gacttgaaac aaaatcggca gtaatgacaa atacaaaaaa actttccaca taacccaaca
      481 ctactacaaa gaaacttttt gtgccaaacc agtctaaaaa tacggttgtg aaaagcagcg
      541 aaagataagc caaaaccaga ataaaatgct ccagccaccg aaaattatct tctttatcac
      601 aatctcgggc gcgtttttga gtgaacatgt gaacaaacag atccggtaat gattttatgt
      661 aaatttttag atttgctttt acttttggtc ttaaaatggt aaaattccac atcctggcta
      721 tattggggat aaaaattatt gcgagcactg caaaaaccac atacatttca aaaagatgtc
      781 cataatgcat catttctgcc aattgaaatg acatggcatt acccaaaaca agtacggcaa
      841 taaataccat tatcagagca gaaatagtga atgtaacgga acgataaagc aaacccgaaa
      901 gtcccgtcca gtcataaact gaagttaacc atcgtcgcaa ggacatcatg agttctcccg
      961 gattggctgt ttgaggacaa ttttcggtac attcaccaca ataatagcat tcccatggtt
     1021 ttaaagaaga tttaatatca ttttcaagtc caagactgct aagtcttacc atttcgcgag
     1081 gaaaagattc ttccggtgtg gacagtgaac atacggcagt acaggtaccg caattgtaac
     1141 aggcgttgaa atttactgct ccgtatttct tcaatttatc ctgaaatgca ggattaattt
     1201 ttgatgccat catttgttca atttataggt aaaatattcg tcattgtcat caatttctcc
     1261 cactgcaacg tcgccatatt taaccagcgt catcaaataa aatgtaacat cctcttttga
     1321 catggatagt ttttccgata gttggggaat ggtcatttct ccatttttca gggcatcaat
     1381 gagtgctttt ttaatcctga caaattcttt aagattttct tttgcctttt cgggaatacc
     1441 ttgtttgtcc cttaaatatt tggccgtttt tcctatttct gcttccatca tttttttatt
     1501 gaaccagtgc atctatcata ctaaaaatct ccttatcgga atagccaatc aaatcaatag
     1561 cattgggtac gcatacagga gcgcacatac cgcaaccctt gcataccgat ttgttgacta
     1621 cagcaattat tttcccatca atttcttttt gttcaattgc ttcaaaagga caagctttta
     1681 aacattcacc gcaccaaaca catgaattac cgttaatttc agcaactatc ggctccgttt
     1741 ctaccaatcc gttactgatg gatgaaaagg cctttgtggc agcagacaag gccgaattga
     1801 ccgattcgct gatatttttt ggcccttggc aagttccggc cagtgttatt ccatcaatca
     1861 ccgtttccac cggacgcaat ttcatgtgaa tctcgttgta gaaattatct ctgccttttg
     1921 gcagtttaaa aagttgaccg atcgactgat caattctggg taacattccg gttaccaaca
     1981 ccactaaatc ggcatccaga agaagttctt tatcggaaaa cagtaaatct ttaactttca
     2041 gggaagtttt tttattcttg aaactgactt caggaggaaa atcatcatga aactggataa
     2101 aaatatcccc attctgtaaa gcttcgtcat aaagtaattc ctgtttgccg taagtgcgaa
     2161 taccccgatg aagatgaaaa ttgcgtatcc ccttgaattt cttttttgtt tcgatagcag
     2221 tatgaattgt cgaaatacag cagaaacgcg aacagtattt attctcacct tcggattgac
     2281 gacttcccac gcaatagata taagcaatgt cgtgaatttc ttttccgttg tataaaagtg
     2341 ttttacctgt cgattgatct gtcagttttt taaaatcggg caaagaaatt acattctcgt
     2401 tattgagata ggaaaattcg cctcgttgcg gaatataatg atcaaaacct gtagcaatca
     2461 ctacagaacc tacgttaatt tccatagttt ctctaagatt gtcaacaggc tgatgttttt
     2521 tttgaagagt cagcgtgaaa tttccaatgc tgccttttga agaaacgact tcggtacctg
     2581 tgagaataga aatattttcg tgttgcagca aatcgttatg aatacgttga acaagctccg
     2641 aacccttttc atttgtcatg aaaagcgatc cccattcagc cacatgccct ccaacagtat
     2701 tttctttttc aatcagcact acagacatat ttttttcggc aatggaaaga gctgctttca
     2761 ttcccgccat tcctgctccg atgactgcaa ttgctttctc tgcttcaaac ttattggggt
     2821 acaaagcttc tgcgtaacgc gattttgaaa tggctgccct taccagtcga atcgcttttt
     2881 cggtagctcc ttctttatcg tccgagtgtg cccaagaagc ctgctcacga atattggcat
     2941 gaacgtaggt atatttgttc aaaccggctg tttcagttac atttctaaaa gtaaccaaat
     3001 gcagttttgg agagcaggat gccactatga ctccatccaa ctgatattga tgaatatctt
     3061 ccaccatttc cttctgattg ctatccgaac atgcaaacat ggtggtttta gctaaaacga
     3121 caccctcttc atgttcaatt gcctgacgta ctttttctac gtcgacataa tcggaaatat
     3181 ttccaccaca atgacagata taaacgccta aattcttttt catcaatctt attaatgttg
     3241 aattaagtaa ctgattgctt cggacgaagc cgaaccggca gatagaatgg aatcgggaat
     3301 atccattgga ccggatgccg taccggcaac gaaaactcca tctatatttg ttttagccgg
     3361 acttcccagt aagtcgctct gccaaatgaa attcaaagaa tcaaattgca acatttcgcc
     3421 atcaaaaatt tgatgaactg actgattgga caacattcct accgacaata ccaccatatc
     3481 gtgttcggct tctttcacta ttccttcctg aatatcttca taacgcagta ttaaattccc
     3541 attttctttt tcggaaattt ttccaatttt tccttttaca aaattgacac ccatatcttt
     3601 ggcttgctgg tagaattctt caaagccttt gccgaaagaa cgaatatcaa tgtaataaat
     3661 ggtaatgtct gccattggaa gcgcacccat taacaattgt gcctgcttca tggaatacat
     3721 gcaacatact tgtgagcaaa caggattacc aacgctgtcg tcgcgcgagc cggtacaaag
     3781 aacataagca atctgatcgg gtatttttcc gtcgcccggt ctcagaatag tgtgataagg
     3841 tctggtagga gctatttctc tttccatttg catggaagta accacatttt tgtattttcc
     3901 atagccgaat cgtggaattt taagcggatc aaacagttga aaacctgtag ccagtacgac
     3961 tgtctttacc tgtaattcaa aacattccgt ttccatagta aagtcgatgc agtgagtagg
     4021 gcaaaccctt tcgcatgcgc cgcacaaagt acattgttca aggtcgataa cagcagcttt
     4081 gggattggct atgctgaaag gaatgtaggc agcttttcga ccgaccagac caaactgata
     4141 ttcatcccga acactaacgg gacaagcttt ttcacacaat tggcaagccg tacagtcttc
     4201 cacatgaacg taacgcggat tctttgtaat ctttgctata aataacgatt cggttttttt
     4261 tacaatactt gtaacttcac tttcagtaaa aactgtaata ttgggatgac gggcaatttc
     4321 agaaactttg ggtgtggtaa tacaggcagc acaatccaat gttggaaaaa ctttgctcaa
     4381 caaaatcatt ttaccaccaa tagaatgatc tttttctacc aatagcacct tataaccgtt
     4441 gtttgcaata ttcagggcag tttcgccgcc ggcaataccc gcaccaataa ccagcgcatc
     4501 gaattctatt ttttttcttc tctcctccat tgtttatttc tccgaataac gggttttaat
     4561 caaatattca ttcagactgc gcacttgcct gacaaaagca tcgctacaaa cggagcaaag
     4621 agcagccatt ctcaagcggg cgggatccaa attgttctgt ttcatttttt cgtgggtcat
     4681 tgctactact ttaccggttt tttcggtgca gctttcgcca aaaacgcaat cggtaccatc
     4741 tgcagcaatg aaaacgccat cgaagccttt ttcgaaagca tgcaggatcc aattgggctt
     4801 tattccgctt gaacatggaa cagttatggt ataaacggtt ggcggataat gcagtttaag
     4861 cagcccggcc atatcaatag ccggatctga aattttctcg gtagaaaata ccagaatttt
     4921 tgcatttttc tttacgaagc tcgtcatgga ggaaaaaatt atttcttttt cagatacaat
     4981 ttataatatc cgttttcctc taccatgcca agaaactcgt ttccttgctt gttgcaccat
     5041 tttggcaaat cattctttgt gccttcatcg gccgacaaaa cttccaaaat ttctcccgat
     5101 tgtatggttc caattgcttt tttagcttcc aaaagtgggc cagggcaggc agttccgcgt
     5161 gcatctaccg atttggctac ggtaagattt ttcaattctt cggtggtcat aattctataa
     5221 tttttaatgt ttgtttatgg gttattactt atatcatcca ttgcatggcg ctttcgccgg
     5281 catctgccac aaaagttgct gctcctgcaa ttcggaaatg tggataatcg ataaattctt
     5341 cttttttgaa gcccatcaaa ttcatggtca tttcgcaagc cgtaatttct attcccaatt
     5401 cggcggcttc cttaaacatt tcgtccaatg aaagtacatg ctgttttttc atcaaatttt
     5461 tcatcatcat gggtcccata ccacacatat tgtgtttgga caatttcagt ttcccatatc
     5521 ctttgggcaa catgcatccg aacattttgg aaacaaagtt tttccctttc acagttttct
     5581 tgggatcacg taatgctgcc aatccccaga atgagaagaa cagttttacc tttgtatcca
     5641 tagctgccgc cgccagcgat ataatcattc ctgccataat tttgtccatg tcgcctgaga
     5701 taattgctat cgacaattgg tcttttttgg ctgaattttc caattgcgtt gttttggctt
     5761 tcaagttttc cagttcagcc aaaagttttt ccatggttat ttcatttgat ttttccataa
     5821 taatagtatt tttaaattga tttctgcaca aattaacgaa aaaatttatg aatctgctat
     5881 atcttacagg aatatttatt cggaaaaatt caaaataaat cggataaaca acttttgaag
     5941 aaggttgtcg gaaatagctt ttttgtatcc gcaaaaaaaa aattaaaaag tcaataattc
     6001 ccatattgac aaacgccatt aatcaaaaag agttttcagc acctccagcg aatgaatctc
     6061 gggaaaatcg ggaagatgaa aacgataata actcactata tcctccaata atttttctcg
     6121 ctgctgacgt gaaagtttga tttcggtcat gtgctcatag tcggtttgca acaactgagc
     6181 cattttcccg gcttcttcgg caaagagata atgccgatga gcaggcggag aggacaaaaa
     6241 aacaccgttt accaaatcga aaaaaagtgt cgtctgtcca tcgatattgg gttcaaagcc
     6301 caagtatcgc gaaagtttaa gtaaaaatac cagatgaaaa ttcgccgttt tttgcttact
     6361 gttatccaaa aactccaatg attgccgcag aaaaaggtag agtgattcat cggcttcgct
     6421 ttgagaaagc actttcaaaa gcacttcggc gataaacatg gcaatagaac gcttgagagg
     6481 atcgatggtc aggctctggt aaggcacaag cacccttaca tccttcattt gctgaatatt
     6541 ctttcgcata tcgtgcgaaa cttccagctc tactatcgac aaaggttgaa aaaaagcagg
     6601 tttccacgaa gactttcttt tccctctgcc ataaatcatg taagcaactc gtccaaaacg
     6661 acgggtaaaa atggttacta tcgcagaatt gtccgagtac tttagtgaat gaaaaactat
     6721 accttcggtt ttgacttgca cagctcgctc ctttttttga aaacaaaatt acgggaaaaa
     6781 tctcgttttt tacctgattc gatgaagaaa ccaatgcaaa acttaatgct aaattctcac
     6841 ttttaagttg aacttagcat ccgtccttcc ccaaaaacaa aaaaaattcg tttcaaaacg
     6901 acatttaaaa tcaagcaaca acccatatcg ctgaaaaatt aaaaaattta aaacaaagtc
     6961 tttgatattt caaaaatctg ccgtatattt gcacccgcaa ttgtgcaaaa cagttgaagt
     7021 tttcataaca tttttggccc attcgtctat cggttaggac gcaagatttt cattcttgaa
     7081 agagcagttc gattctgcta tgggctacca tattacaaca ctttaatatc tcaaaaaaca
     7141 atggctaaca ataattcagc tattaaaaga attcgtcagg caaacaaaaa aaggcttcac
     7201 aaccgctatt acatcaaatc aacacgtacg gcaattaaaa gattgcgtac aactaccgat
     7261 aaagaaaaag ccgcagaatt gttgccacaa gtttcttcca tgctcgacaa gctggctaaa
     7321 cgcaatatga ttcacaagaa caaagccaac aacttaaaat cgaaattggc tattcatgtg
     7381 aacaagctgg cgtgaaccga taaaaaatga atttttaaag ctcttcatcg acaggtgaag
     7441 agctttttta tttacagttt ctcgccaaaa gccaaatctc cggcatcccc caaaccagga
     7501 atgatgtaag aatgactgtt tatttcggga tcgatggcag cagcccagat agtggttata
     7561 ttttcaggga aatgggtagt gagataattt atcgcatggc ggctggcaat aaccgtagcg
     7621 atatgaattt tagagggaaa tcctttggtg gttagcgctg aaaaagtctg ctccatcgag
     7681 cttccggtag ccagcatggg atcagtgaga atcaacgttt ttccttccaa accgggagaa
     7741 gcaatgtatt ctacaaaaat ttcaaaattt tccgcattcc tgtattttct gtaagctgag
     7801 acaaaagcat tttccgagcc atcgaaataa tttaaaaaac cttgatggaa aggaaggccg
     7861 gcacgaagaa tagtagaaat taccactttt tcgtcgataa ctttcacggg agcaatccct
     7921 aaaggagtct gaactttttc ctccacataa tccatagctt tgctgatttc ataagccatg
     7981 atttccccaa ttctctccat attccgcctg aaacgcatgg aatctttctg aatttctacc
     8041 gaccggattt cttttataaa ctgatttaaa attgaattgt tatcggacag atttataatt
     8101 ttcatatatc gacaaacatc aaataattat tttctcttct ctctctccat caaaacaaca
     8161 gtttttttac tgtttatcct tttctcttat ttccacacgg cgaatttttc cgctgatggt
     8221 tttgggcagt tcgtccacaa attcgataat acggggatat ttatagggag cggtaacttt
     8281 tttcacatga ttttgaagtt cttttacaag ctcctctcct gctttatcct tgtattcttt
     8341 ggcaagtatt accgttgctt tcactacttg accgcgcaat tcgtcgggta ctccggtaat
     8401 ggcacattca acaactgcag ggtgagtcat cagtgcactt tccacttcga aaggcccgat
     8461 acggtaaccg gaacttttga tcacatcatc cgttctacca acaaaccaga aataaccctc
     8521 ttcatctttc caagccatat cgccggtatg ataaacattt cccctgaaag cttcacgtgt
     8581 cagttcttcg ttccgataat aacctttgaa caaacccaca ggataatttt tgtgcagctt
     8641 gattacaatt tccccttgtt cacccacttc agccgatcgt ccatccggca tttgaatatc
     8701 cacatcgtaa tgaggtcccg gcaatcccat cgaaccgggt tttggttcaa tccaaggagt
     8761 ggtatataca caaactgtcg tttcactttg cccgtaaccc tcgtgcagtt taatgcctgt
     8821 caactcgtaa agtttttcgt aaacggaagc attcagggct tctcctgccg ttgtagcata
     8881 ttccagagaa gacaaatcat acttgctaat gtcttccctg atcagaaaac ggaaaatggt
     8941 tggaggcgca caaagagaag ttacatgata tttctcgatc atttcgagca tatttgcggg
     9001 agtaaatttt tcgtggtcat atacaaatac acaagcaccc acaatccact gtccataaat
     9061 tttcccccaa agtgctttca accatccggt atcggctatc gtcagatgaa tactgttttc
     9121 atgcagattg tgccagtatt tggctgtaat aatatgtccc aacggataaa gacaatcaag
     9181 caacaccatt tttggattac ctgtagttcc ggacgtaaaa ctgataatca acggatcggt
     9241 attttcattc acatgatgaa tgggtgtaaa gggagtcgcc tcatcaattc ccttgtgaaa
     9301 atcgagccaa ttttccggca cgataggccc aatggaaatc agcatttcaa cagtagggga
     9361 ttcagccatt gaatcgttaa tgtggcttac aatcggttct tctccaactg caactattgc
     9421 cttaatatca gcagcattgt ttcggtagat caagtctttc ggcgtaagca aatgcgtgtc
     9481 tggaatagta atagcgccca atttatgtaa agccagaata gtaaaccaga actcatatcg
     9541 acgtttgagt atggccatca ccacatcgcc ttttcctata cccaaacttt ggaaataaga
     9601 agcagtctgg tcactgtatt ttttcatttc agcaaaagaa aattttctgc attctccctg
     9661 atcattggtc caaatcatgg cgagtttgtc cggctctttg gcagcccact cgtctatcac
     9721 gtcgtaagca aaattaaaat gctcgggaac gatcaattga taattagcct taaaatcatc
     9781 aaaatcctta aattcagttt gttttaaata tttttccaac atattttgaa tagaataaat
     9841 tactaacgat aagttcaatt aataaaattt gctacgtctt ttaaaattat tttttatccg
     9901 aaaaaagctt tctttcgaga ctattaatta aatgataaca gccagaaatt tgactctttt
     9961 cccgttcaaa gctttcattc cgtgtgggcg tgttgcatca aagtaaatgc tgtcgccttc
    10021 gttcagaata aggtcgttgc cggcgatgcg caatagcaaa gtgccttcca gcacataatt
    10081 aaactcctga ttgaaatggg tattcaatgt aagcggattg tcgttgggtt ctacggtaac
    10141 ctcaaaaggt tctgccttgg gatgacgaaa accgtaagca agtgcctgat atttgtaagc
    10201 ttttgttctt tctacgcttg ctcccgttcc cttacgggta acgaaaaaaa cctgtgcgtg
    10261 aggatcatcg ccaacaagaa gagcagtagt tgcaacgccg aaagaatcgg cagcctgaca
    10321 gataaaactc atcggtatat cgtaatcgcc cgattcgtat tgttgatatt cttctgttgt
    10381 agcaccacat ttttctgcag cctgttcggg agtcagctca agtgattcgc gcaatgcacg
    10441 taatctttcg gcaatttgtt tgatttgaat attcatggat gtaaaactta aaaaaaattg
    10501 ttttttgcag aatctatttt ttcatttgga ataagatgct ttcaaccctt tgataacagc
    10561 atttgaaaat ccattggctt ccatttcgtt caaacctttg atggtaaagc ctccgggcgt
    10621 agtaacctta tctatttccg tttctggatg agactgatgg agttcaagca aatccacagc
    10681 acctcttacg gtttgaataa ccgcttcctg agccatctga ggcgaaatgc ccatttcaac
    10741 agctccggct accgaagccc gcacataccg aaaagcataa gcaatgccac atgaagttaa
    10801 tgccataaaa gcatccattt gattttcgga cacgaaaaat gttttcccta aaacattaaa
    10861 aatttccagt aacaactgtt ggtgttcttt gcaggcattg aacgatgaaa caacagaaac
    10921 accctgtcct atttcgaccg ctacattggg aataacacgg aaaaacactc cattagaatc
    10981 aaaaaaatgc gtcagggttt caaaactaac gcctgccgca atggaaatga ttacttgctt
    11041 tttataatcg atgtacttgc ttatttcttt ggcaaccgct tcgaccagcc aaggcttgat
    11101 agcaagcaaa acaatatcgg cctgcctgat gatttcccta ttagaagtac ttacggtaaa
    11161 atgtggatta aaagcagtca attgatccaa cttttcttga gaaatatcgc taatggcgat
    11221 attttcagca cttaaaatgt ctgattttga ccagccgcgt gccaatgctc cacccatatt
    11281 tccggctcca ataatggcaa tttttaaatt tttgatttcc ataagctcat taaaatgata
    11341 aatatccgag aacttcgtga accaaatgca aacttacaca aaattttgga cttattttct
    11401 gcagcgaaac aaaaagtatt ggttcaactg caattctttt tagcagcaag aaaactttaa
    11461 attgtttcaa tttaaacaat tattctaact tgctgatttt tatttaattg ttctaaacat
    11521 tttaaaatat ctatcaaaaa cattccaaaa aaatattttc acaagatttg gtaatgaaaa
    11581 tttaaacata agggctcgaa tatttgtttt aaaagtaaaa tcaaatctca aacttatgaa
    11641 aacaaatgta ggctcgaccg acaaagtagt acgcttagca ttggctatcg tgctgattgt
    11701 tctgttttac aagggtattt tctccgtcat tctgggaatt gttgctctta ttgtcgcttt
    11761 tgtacttact ctaacctcgc tgattgggtt ctgcccgctt tacacacttt ttggaataaa
    11821 tacagcaaag aaaaaagagc agagtgaaaa cgctgaacaa cccactaaaa aacgacaacc
    11881 caagaactga ggacatcgat gacgtcagga atcggaattt tcggtacacg aaattgagtt
    11941 atctccatgc atttcattcg acaaaggata caaatgcttg taaaagggaa agggagaaaa
    12001 aaatcttcca attacttaaa aatttcttta attaaacaac ctgttccttt gaaaatcgga
    12061 caaaaattcg taaatttgtg cggtttttta agtaactaat ctctaaactt aaataaaatg
    12121 attaaagtag gtattaatgg ttttggccgc attggacgtt tggttttccg tgcagcccag
    12181 gaaagaaacg atattgaagt cgttgctgtg aacgatcctt ttttggataa ggattatctg
    12241 atctacatgt tgaaatttga cagcgtacat ggcaaatttc agggaacagc cgaaaataag
    12301 gatggaaaat taattatcaa cggaaaagaa gttcttttct ttgcagaaaa agatcctgca
    12361 aacattggct ggggagctgc cggtgctgaa tatgtagtag aatcaaccgg tgtattcacc
    12421 actgttgaaa aagcttctgc tcacctgaaa gccggtgcaa agaaagttgt tatttctgct
    12481 ccatcgaatg atgctcccat gtttgtgtgc ggtgtaaacc ttgacgcata caccaaagag
    12541 atggatatcg tttccaatgc ttcatgtact accaactgct tggctccact tgccaaagtt
    12601 attcacgaca atttcggaat tgttgaaggg ttaatgacaa ccattcatgc cgctaccaac
    12661 actcaaaaaa ctgttgatgc tccttcaaat aaagactgga gaggtggccg ttctattttg
    12721 aacaacatta ttccttcttc tacgggagct gcaaaagctg ttggaaaagt tattccttca
    12781 ctgaacggga aactgacagg tatgtcaatg cgcgttccga ctgctgatgt ttcggtagtt
    12841 gaccttaccg ttcgtttgga aaaagctgct acctacgaag aaattaaagc tgccgtaaaa
    12901 gctgcttctg aaaacgaaat gaaaggtatt ttgggttaca ccgaagatgc tgtggtttca
    12961 accgatttca ttcacgatcc cagaacgtca attttcgatg ccaatgccgg tattgcattg
    13021 aacagcaatt tcgttaaact gataagctgg tacgacaacg aatggggtta ttcaaataag
    13081 gttctcgact taatctgcca catggacagc gttaaataaa gagctttaac agaaaaaaaa
    13141 gaatagtttt tttgaactct ctcaaaatta atttttgagg gagtttttta ttcaattact
    13201 cccaacaaaa aagggaacag cttgcgtggt ccccttttta atatgattaa caaaaaatct
    13261 tcaattttat tcttctgtcg ttggtgcctg attttcggca ggtgcctgtt caacaggttt
    13321 aggagccgaa acttccgaag tggcaggtgc tgcctgaact tcagtttctg ctttctttct
    13381 agaagaagaa cgacgtgtac gagttgtttt cttggtagtt ttatccttga gcatgttttc
    13441 gttgtaatca acaagctcaa taatgcacat ttctgcatta tctcccaagc ggaaacccgt
    13501 ccggagaata cgcgtataac cacccggacg atttgcaatt ttcaccgaaa tattttgaaa
    13561 caattcggtt acagcttcct tgttctgcaa ataactgaaa accaaacgtc ttgattgagt
    13621 cgtatcttcc ttggctctgg tcaaaagagg ttcaacgtaa actcttaaag ctttagcttt
    13681 aggaagtgta gtggagattc ttttgtgcaa aatcaacgat gaagccatat tggacaataa
    13741 agctttcctg tgagaagacg tacgacttaa atggttgaat ttcttattat gtctcatttc
    13801 atttaatctt tatctaattt atactttgaa acatccatcc cgaaggaaag atttaaacta
    13861 atcaattttt cttccagttc ggcgagcgat tttttgccga aattacggaa cttcaacagg
    13921 tcgttgcgtt gaaaactgca aagttgtccc aatgtttcaa tatcggcagc tttcaaacaa
    13981 ttcaatgctc tgactgaaag ttccaaatcg gtaagtttgg tttttaacaa ctgacgcatg
    14041 tgaagaacct cttcgtcaaa ttcgcttgtt gtttcacctt ctactgtttc aacttctatt
    14101 ttctcatcag agaacaacat gaaatgctga atcagaattt ttgctgcctc tttcaatgct
    14161 tccttaggag gaatagaacc atcggttaaa atttcaatta ctaatttttc gtaatctgta
    14221 acctgttcca cacgataatt ttcgatggca tatttcacgt tacggatagg tgtaaaaatt
    14281 gaatcgatgg gaataacgcc aatttcgaga ttggaagttc tgttttcttc agccggtaca
    14341 tatccacgtc ccttgttgat ggcaatgtca atctgaaaac ttcctttcgg atcaagttcg
    14401 caaataataa gctccggatt gagaacttca aaaccggtaa gatgtttacc aatatctccg
    14461 gctttaaaaa ctgctgtatc cgaaacggta atcgaaactt tttcgttatc aacaccttct
    14521 accacttgtt tgaaacgcac ttttttcagg ttgagaatga tattggttac atcatcaatc
    14581 actcccggaa tggtagaata ttcatgatct attccttcga ttttgataga agtgatggca
    14641 aaaccttcca aagaagacaa gagaatacgg cgcaatgcat tgccgatagt aatgccatag
    14701 ccgggttcca gagggcgaaa ttcaaatttt ccatgaaaat cgtctgcctc cagcataata
    14761 accttttcgg gtttttgaaa tgctaatata gacatgaatt tacaaattat ttagagtata
    14821 actcaacgat taaatgttct ttgatatttt cagggatatc ctctctttcg gggatatgca
    14881 gataagttcc tgaaagcgtt gaagcattcc attcaatcca cggatatttg ctgtggttga
    14941 aaccgctcaa cgaattctga atcacttcca ttgatttgtc tttttcgcgt acacccacca
    15001 cctgaccagg tttaacctga taggaaggaa tgttaactac cttaccatcc actgtgatgt
    15061 gtttgtggtt taccaactga cgggcagcag cacgtgtagg tgcaattccc attctgaaaa
    15121 ccaaattatc aagacgcgat tccagcattt gcaacaaaat tacgcctgtt acgccggaag
    15181 cacgacgtgc acgttcataa atgatatgaa actgcttttc aagaactccg taggtatatt
    15241 tagccttttg tttttcgcgc agctgaatac cgtattcaga agttttcttt cttcggttgt
    15301 tcccgtgctg tccgggagga tagttctttt tgctcaatac tttatcgggt ccaaaaatag
    15361 cttcgccaaa cttacgtgct atttttgttt taggtccaat atatcttgcc attttttcta
    15421 aaattgattg ttataattaa tttattcggg tatccacatt gtaaaaactg cttcagccgc
    15481 cattgcagag aggaaaaaac ttgcaatgaa ttttttaaat ttttacctgt cggcattacc
    15541 ctttttgatt catacaaaaa gtggaaaatt gatattatac tcttctcctt ttgggtggac
    15601 gacaaccatt atgtggaaga ggtgtaacat caacaatctc agttatttcg atacctattc
    15661 cgttaacggt tcggatagct gattcgcgtc catttcccgg ccctttgaca taggctttta
    15721 ccttacgtaa acccaagtcg aaagctattt ttgcacaatc ctgagctgcc atttgcgcag
    15781 catagggagt gtttttcttt gaacctctga atcccatttt tccggcagag gaccaagaaa
    15841 taacttcacc attttcgttg gtaagagaaa taataatgtt gttgaaagat gaatgaacgt
    15901 gcaattgacc tgcggcatcc actttcacta ctcttttttt ggctgcaact gttttctttg
    15961 ccataattat ttatatcttc tttttctgat gattataatt tccagttatc tgttatttgg
    16021 ttgctttttt cttgtttgca acagttttct tcttcccttt tcgagtgcgg gcattgtttt
    16081 tggtgctttg acctctaaca ggtaaaccga gacgatgacg gatgccgcgg tagcaaccta
    16141 tatccatcaa tcgtttaatg tttaactgaa cttctgagcg caaatctcct tctaccttgt
    16201 attcggaaga aattatctcc ctgatttttg cggcttgttc atctgtccag tctttcactt
    16261 ttatgtctct gtcaacaccg gccttactta aaattttatt tgcgctgctg cgaccaattc
    16321 cgaaaatata agtcaacgca atttcacctc gcttgttttg aggtaagtct actccaacaa
    16381 ttcttattgc catattaaaa tttttttcat gtaaattaat taaccctgac gttgtttgaa
    16441 tcggggattc tttttattaa tcacgtaaag acgacctttt cggcgaacga ttttacaatc
    16501 ttcagaacgt ttttttatgg atgctctcac tttcatctct gatagtattt ttttatttgt
    16561 atctaaatgt aattcttcct ttggtcaaat cataaggcga tatttcaact ttcaccttat
    16621 ctcccggtaa aatttttatg taattcatcc tcattttgcc ggaaatatgg gctgtaataa
    16681 tgtgaccgtt ttcaagttca acacgaaaca tggcattcga taatgcttct attatcgtac
    16741 catcctgttc tattgctgtt tgtttggcca taaaaattaa attgctcttt ctcctaaaac
    16801 ttgttcaatg tattcaaaac ttgatagtat atctgctttt ccctgacgaa tggctacggc
    16861 gtgttcaaaa tgtgccgaag gctttctgtc cagcgttcgg gtagtccatc catctttttc
    16921 aaaaacaata tgtcgtgttc ccatattaat catgggctct atgcaaatca ccattccgta
    16981 tttcagcatt acgccgttgc cttttcttcc ataattcggt acctcaggtg cttcgtgtat
    17041 ctctctaccc agaccatgac caaccatttc acgcactacg gaatagccgc gttgttcaca
    17101 ataactctgt acagcataac caatatcacc agtacgatgt ccttcctgag cctgctcaat
    17161 acctttataa agtgattctt tggttgtttt cagcagattg acaatctgtt gatctacttc
    17221 tccaacacag aaggtataag cagaatcgcc atgaaaaccg ttgatataag caccacagtc
    17281 aatcgaaatt atatcgcctt cctttaaaaa aactttatca gaaggaattc catgtactat
    17341 ctgttcgttt accgaagtac agatggaatt aggatatccg ttataatcaa gaaaactcgg
    17401 cactgcacca tgatcacgaa taaattcatc ggctattcga tcaagttcca acgtactgat
    17461 tccgggtgta atatgttttg cgagttcagc taacgtttta gctaccaatt gattactgat
    17521 tctaagtaat tctatctctt cttccgattt gagataaatc atttttaatg ttatcttatt
    17581 taataagcag aaactccact tgttcgtcct ttgatacgtc ctgacttcaa taatccgtcg
    17641 taatgtcgca tcaacaaatg actttcaatt tgttgcaaag tatccaacgt aacaccaact
    17701 aaaattaaca gggaggtgcc tccaaaaaat tgtgcaaagg catggtttac accgaaaata
    17761 ctggcaaacg caggcataat ggcaatgata gccagaaata ttgaaccggg caatgtaatt
    17821 ctggacataa tcatatcaag atattcagcc gtctttttcc caggttttac acctggaata
    17881 aatccattat tacgtttcat ctcttctgcc atctgcgttg gattaatagt gatagccgta
    17941 tagaaatacg taaacaaaat aatcaggatg gcaaaaacaa agttgtacca gaaacctgta
    18001 ttatccataa aagcaccaac aaatccctgc attgactcgg attttgaaaa tccaacaatt
    18061 gtaatgggaa taaacatgat agcttgagca aaaattatcg gcataactcc ggctgcatta
    18121 attttcagcg ggatatactg ccttacgcca ccatactgtt tatttcctac cacacgtttt
    18181 gcatattgaa caggaacttt acgtgttccc tgaaccagca ttatagaagc tccgataacc
    18241 agtaaaagga aaagaatttc tataataaac atgatcagac ctccgcccac atcggtcaat
    18301 cgagaggcaa attcccttac aatagcttgc ggcagacggg caataatacc gactaaaata
    18361 ataaacgata ttccgtttcc aattcctctg tctgtaattc gttcaccaag ccacataaca
    18421 aacatactac ctgcacagag aataatggta gaagttgcct gaaagaaaaa acctgatgga
    18481 agtgcggaac cagcctgcct taactgaaca cttaaattaa ttaaataaga aggtccttgc
    18541 aagagaagaa ttcctactgt aagataacgc gttatctgat tcatttttct tctgccactt
    18601 tcaccttcac gttgcatttt ttgaaaatat ggtactgcta tagtcagcaa ctgaataacg
    18661 atagaagccg aaatatacgg cataattccc aacgcaaaaa tggacgcatt ggcaaacgct
    18721 ccacctgaaa acatatccaa taaagcaagt agtcctccgc ttgtttgagc tttaagagct
    18781 gttaatgctc ctgcatcaat acccggaagc acaacataag aaccaaagcg ataaatcatg
    18841 acaaacaata atgttgtcaa taacctgttg cgcaagtcct ctatcttcca aatattttta
    18901 agtgtctcaa tgagtttatt catcttatga aatttttact gccgttccac cggctccaac
    18961 aattgcattt tcggcagatt ttgaaaaagc atgagcttca acatctaatt ttacggtcaa
    19021 ttttccgcgt cctaagattt ttaccaactg atttttagat ataaatccgg cttgacgaag
    19081 atcatcgatc ccaattttag ataattgatt ttcttgagct aaaatttcta aagtatcgat
    19141 attgattgct ttatattcaa ttttatttat attcttaaaa ccaaacttgg gtaaacgtct
    19201 ttgaattggc atctgcccac cttcaaaacc gattttgtgc gcataccccg agcgagattt
    19261 tgccccttta tgtccacgtg tagatgtgcc ccccattcca gatccagaac ctctaccttt
    19321 tcttttattt gattttaccg agcctgcagc tggggttaaa ttatgtaaat ccatatctcc
    19381 tttttattaa agattaaaat tttttaatcg actatcttta ccaaatgttt aactttctca
    19441 accattccca aaatttgtgg agtcgcttca tgttcaacaa catcattgat tttatgtaat
    19501 ccaagtgctt ctaaagtcaa tttttgaact ttaggagatt ttattttact tttaacttgt
    19561 ttaattttaa tagttgccat gattatctga aattatccat taaaaacctt atccaaagaa
    19621 acctttctgt tctgagcgac agtgtatgca tcgcggagtt cggagagggc agcaatcgtg
    19681 gctttcacca ggttgtgagg attggatgaa ccctttgatt ttgctaacac atccgtaata
    19741 cctacacttt cgagcactgc acgcattgca ccgcctgcag ttactccggt acctgaagaa
    19801 gctggtttga taaaaacgcg cgcaccacca aattttgctt cctgttcgtg aggaatagtc
    19861 ccattcagca ccggcacttt gattagattt ttcttggcta cttctgttcc tttagcaatt
    19921 gctgcggtaa cttcgcttgc ttttccaagt ccccagccta cgatcccatc ttcatttcct
    19981 accaccacaa tagctgagaa gctaaaagtt cgtcctcctt tggtaacttt ggttacccga
    20041 ttgacagcta ctaatctgtc ttttaattct aaatccgttg ttgtttttac tctattcatc
    20101 atttttgcca tacattctta aaatttaaga ccgccttcac gagcagcatc ggccaattgt
    20161 ttaactctac cgtgatataa ataaccattg cggtcgaaca ctacttcagt gataccggct
    20221 tccttagcag cctgagcaat tagagcaccc accttaccgg cttgctcttg cttggttatt
    20281 ttttccttaa tagctaatga cgaagctgaa gccaatgtta ttcccttttc atcatcaatt
    20341 agctgaacat aaatttgagc gttgcttcta aatacggtca ttcggggtcg ctgagcagtt
    20401 ccaaaaattt ttttccgtat atgagccttt attttttgtc ttctgtctaa ttttgtcata
    20461 acttcacaaa tttacgtata ttatttaccg gctgattttc ctgcttttct tcgcagtact
    20521 tcaccctgga atttaattcc ttttccttta tacggttcag gtttacggaa agaacgtatt
    20581 tttgcacaaa cctgtcccaa aagctgctta tcgcaacttt ccaaaataat cagcggattc
    20641 tgattacgtt ccgactttgt ttctacctta acttcgggcg gtacttttaa aaaaatactg
    20701 tgcgaatatc ctaaagcaaa ttccaaaacc tgaccctgag cagaaacacg atagcctaca
    20761 cctaccaact ccatagtctt tttaaagcca ttagacactc cttcaaccat attgttgatc
    20821 aaagaacgat acagcccgtg catggcacga ttttctttct catcatcgat gcgggttaaa
    20881 gtacaaaaac catcttttac ttcaactgaa atatttggat gaatttgctg acttaaagtt
    20941 cccttaggcc cgcttactga aaccaaattg ttttttatcg taacgttgac tccagccgga
    21001 atttttatgg gtaattttcc tattcttgac atatcatttt cctccgcctc ttaataaaca
    21061 taacataaaa cttcgccgcc aattttcaat ttggctgctt ccttattggt cataacgcca
    21121 ctggatgttg aaaggatagc tatacccaat ccatttaaca cgcgaggtat atcctggtat
    21181 ccaacatatt tccgaagacc gggtttagat actctgatta atttctttat tgaatttact
    21241 ttgttaacag ggtcatattt caacgcaatt ttgattgttc cctgtggacc atcttcaaca
    21301 accttgtaat tcaggatata gccttgttca aataaaatac gcgtaatttc acgctttaaa
    21361 tttgaagcag gaatttctac aacccgatgg tttgctttaa ttgcatttcg caaccgagtt
    21421 aaataatctg ctattggatc tgtcataaat gtttaaactt taaattaatc tcgactgccg
    21481 agagaatatt taatatatgg taaaattcta aatgtaattt ttataaaccg aaaaatcact
    21541 aatccaggat atttttgaag ggaaacttac cagctcgcct tctttacgcc gggaataagt
    21601 ccttttgaag ccatttcacg aaatacaata cgagatacac caaactgacg gatatatcct
    21661 tttggacgtc cggtaagctg acatctgttg tgcaaacgta cgggagaagc attcttgggt
    21721 atggattgaa gcgcttcata atttccttcg gccagcaaca gacttcgctt ggcctcgtat
    21781 tttgccacca ttttgtttct ctttacctcg cgggctttca ttgattcttt tgccattttt
    21841 attccttttt taattttttt tgaatggtaa accaaactct tttaaaagag caaatccttc
    21901 ttcatcagtt tttgcggtag taacaaaagt tatgttcatt cccagtaatc gactaatgga
    21961 atcgatattg atttcaggga agataatttg ctccgtaatt cccaatgtgt aatttccacg
    22021 accatcgagt ttgttttcta ttcccttaaa gtcacgaata cggggaaggg caactcgaat
    22081 cagacgttcc aaaaactcat acattctgtc cccacgcaag gttactcgaa cgccaatggg
    22141 catttttttt cgaaccttga aatttgaaat atcttttttg gaaacggttg ctacggcttt
    22201 ttgtccagta atggtcgtca tctcattgat tgcggtatca ataatcttcc tgtcggcaac
    22261 ggcaataccc aacccttgat taagaacaat tttttccaac ctgggaacct gcatcaccga
    22321 tttgtaagca aattccttca ttaaagccgg aacaattcgt tctttgtatt cctgttttaa
    22381 acttgctgta ttcattctta aatttcctct cctgtctttt ttgaaattcg aactaattta
    22441 ccttcattat tcagttttcg accaacacgc accggtcgac cattttctac cggatttaaa
    22501 ttggaaatat gaatgggcgc ttcttgttta acaataccac cttgtggata tttggcattg
    22561 ggtttcatgt tttttgacac caaattaatg ccttcgacaa tagcacgttg ttccttaaca
    22621 agcacttcca atacgcgtcc ggttttacct ttgtctactc ccgtatttac gtaaactata
    22681 tcgccttttt taatatgtaa cttcgtcatc tctatataaa tttttcttat gttaaagcac
    22741 ttccggtgcc aaagaaatga ttttcatatt ggtggcacgc aactcacgag ctaccggtcc
    22801 aaaaatacga ctcccacgaa gttcaccagc attgtttaac aatacacacg cattgtcatc
    22861 aaagcgtata tatgaaccat caacacgtcg aacttctttt tttgttcgaa ctactactgc
    22921 ttttgatacc attccttttt taatgtctga tgaaggtatt acacttttta ttgaaacgac
    22981 aatggtatcc cctactgttg catatcgctt gccggttccg cctaaaactc ggatgcacaa
    23041 agcctctttg gctccgctat tgtcggcaac gagaagtctt gtttcttgct gtatcataat
    23101 tatttggctc tttctattat ttcagtcaat ctccatcttt ttaacttact cagcggacga
    23161 gtttccataa tgctgacggt atcacctata ttgcattcgt ttttttcgtc gtgagcataa
    23221 tactttttgg ttttatttac aaatttaccg taaatagggt gtttttcttt ccaccttaca
    23281 gctacagtta tggttttatc cattttgttg ctggtaacta caccaattct ttcttttctt
    23341 aaatttcttg tttccatcgg gtgtacttaa tttattgatt aatttctcgc agacgcaatt
    23401 ctgtagcaat acgtgcaata tttctacgaa caacttttat ttgcattgga ttgtccaatg
    23461 gactgatcga atgatttagt tttaaacgaa ctaaattttc tttttcagtt tctaatcgtt
    23521 ccaatagttc tttggtgcta agttctctga tttctcgcgt tttcattttt tacaaatttt
    23581 gatctgtgag atcataatct cttcttacta caaattttgt tataatgggc aatttttgag
    23641 cacctaaacg taatgcttct tttgcaacgg catagggtac tccatccact tctataatta
    23701 tcctgccagg cgttattgga gcaacaaaac cttcgggaga acctttacct tttcccatac
    23761 gtacttctgc aggttttttg gttataggtt tatcgggaaa aacacgaatc caaacctgac
    23821 cctgacgttg catataacga gttacagcaa tacgtgcagc ttctatttgt tgtccggtca
    23881 accaaactgt atccatagat ttgataccaa atgatccgaa agccagttga ttcccgcgct
    23941 gcgcatttcc tttcatgcgc cctttctgct gtcttctgta ttttgttttt ttaggttgta
    24001 acatgatgcc ttatatcaaa ttatattttt attttttctt tcttctggaa gaatttttat
    24061 ttccatgatc gcctcccgag tggtggtctc ccgaacgacg ttcggtacgt gagccttctt
    24121 tttgtcctgt aacataggaa ggagccaaat cgcgcttacc gtaaacttcg cctctgcaaa
    24181 tccacacttt tatgcctatc aaacctactt ttgtcaaagc ttcggcgcaa gcataatcaa
    24241 tatcggctct gaaagtatgc aacggtgttc tgccttcctt gtacatttcc gaacgtgcca
    24301 tttcagcacc gttcaaacga ccggaaacct gaactttgat tccttctgct cccattcgca
    24361 tggtagaagc aatagccatt tttacggctc ttcggtatgc aatttttcct tccagctgat
    24421 gagcaatgtt actggcaact atcactgcat caagttctgg gcgcttgatt tcgaaaatat
    24481 tgatttgaat ttccttatcg gtaattttct tcaattcttc ctttaactta tctacttcct
    24541 gtcctccttt acctataatg ataccgggac gagccgtgca aactgttatg gttacaagct
    24601 taagggttcg ttcgataatg attcgggata cacttgcttt ggataggcgg acatttaaat
    24661 attttcttat cttactgtct tcgagcaagg tgtcgccata ttttttccca ccgtaccaat
    24721 ttgaatccca accacgtata attcccaagc gattgcttat tggattagtt ttttgtccca
    24781 ttgagctatt taaatttgat ttgtatcgtt attatttctt gaatccacat ataaagtaac
    24841 gtgattggaa cgtttgcgaa tacgataacc gcgtccttgc ggagccgtac gtaatctttt
    24901 taacgtagtt ccttcattca catatacttt gcttacaaac aaattagcat tatcggcttt
    24961 ttgttcggtc ttgatttccc aattgctgat ggccgattta agtaatttct ccattctgcg
    25021 agaagcttcc tttgatgaaa atttcaacac acttaaagcc ttatttactt ccattcctct
    25081 gatcatgtcg gcaacaagcc tcattttgcg cggagaagta ggaacgtcat tcaacttagc
    25141 gaaatagagc gtttgacgag cttcttttct tctatcagca gatatttttt ttctagcacc
    25201 catttttacc aattttttta attttctaac ttactgcttc aatggtttat tttttctttc
    25261 ctgcatgtcc acggaaagta cgcgtcagtg caaattcacc cagtttgtgt ccaaccatgt
    25321 tttcggtgat atacaccgga ataaatttgt ttccattgtg aacagcaata gtatgaccca
    25381 caaaatcagg cgaaatcatg gaagcacgtg cccatgtctt gacaacggtt ttctttccgc
    25441 tttcattcat gtcaagtatt ttcttttcaa gtttcagact gatataaggt ccttttttta
    25501 atgaacggct catattgatt tacttcatta ttgaattatt ttttccttct ttcaattatg
    25561 tacttgctgg actgcttttt gggatgacgt gttttgtacc ctttagccaa aagtcctttg
    25621 cgcgaacgag gatgaccacc ggaagcacgg ccttctccac cacccattgg gtgatctaca
    25681 ggattcatta caacacctct cacgcgagga cgtctaccca accaacggga gcgacctgcc
    25741 ttacccgatt tttccagcgc atggtctgaa tttcctacac ttcctacagt ggctttgcat
    25801 gcaccaagaa tttttctgac ttctcccgaa ggcaatttta taatgacata tttatcttca
    25861 cgggaaacca actgagcaaa tgtacctgct gaacgggcta ttgcggctcc ctgaccggga
    25921 cgaagttcta tattgtgaac tatcgttccg agcggaatgt tttccaaagg aagtgcatta
    25981 cccacttcag gtgctacatt gctgcccgaa ataattttct gacctacctg caaaccgttg
    26041 ggagctaaaa tgtatgattt ttctccatct gcataataaa gcagtgcgat gcgtgccgaa
    26101 cggttgggat catattcaat agccttcact gtagccggga ttccatcttt ttttctggta
    26161 aaatcgataa ctctgaattt ctttttatga ccaccaccaa tataacgcat ggtcatctta
    26221 ccattattat ttcttccacc ggtcttacgt tttccgtaaa ccagggattt ttccggttct
    26281 cttttcgtaa tagtgtcaaa cttgctaata attttatttc tctgccccgg tgttgtgggc
    26341 aataatttac gtacagccat tttagttttt taaatattgc taaaaaaatc gatttcttct
    26401 ccttttttta cagtaacaat agctttttta taagccggtc ttttccctga tattactcca
    26461 ctctttgtat agcggctccg ttttttggaa ggaacaatca ttgtgttgac atcaactacc
    26521 gttacgttgt acatggtttc aacagcctgc ttaatttgca gtttattggc atccttctgt
    26581 accttaaagc catagcgatt gaacttgtcg ccaagctttg tcaatttttc tgttaccaat
    26641 ggttttaata tgactcccat ttctatattc ctccttattc tactttaaaa atttgctcca
    26701 aaatagtaac cgatccttcc gtcaatacaa gagtttttgc attgagtaca ttgtaagtgt
    26761 ttagttcaga agtcgttatt acatttattg aaggtaaatt acgagccgac aaatatacgt
    26821 ttttatttgc acttgataaa acaattaaag gctttttatc agcaacttgt aaatttttag
    26881 ccaattcgag catttttttt gttttcggag cttcaaattc aaaattttca actacggtaa
    26941 ttgctccatt tttggcttta taagaaagag cagatttgcg agccaactgt ttaacttttt
    27001 tattcaactt gaagctgtaa tcgcgtggaa ccggaccaaa aacacgtccg ccaccaactc
    27061 tgagaggaga tttaatatcg cccggacgag caccgcctcc gcctttttgt cttcccaatt
    27121 tacgggtgct gcccgaaatt tcgctgcggg ctttagcttt atgcgttccc tgacgtcggt
    27181 tggccaaaaa ttgttttaca tccaaataaa tagcatggtc gttcggttca atgccgaaaa
    27241 tagcatcatt caacgacact tttctgccgg tgtcttctcc tttgatattt aatacactta
    27301 attccatgat tatttattga tgattacgat tgaaccttta gctccgggaa cagaaccttt
    27361 gaccagcaat aaattatgtt ccggaataat ttttaatacc tgcaaatttt gaacggttac
    27421 tttatcgcct cccatgcgtc cacccattct cataccttta aatacacgcg acggaaaaga
    27481 agaagctcct aaagaaccgg gcgctctctg tcggttgtgc tgaccgtgag ttgtctctcc
    27541 aactcccgaa aatttatgtc tttttacaac gccttgatac ccttttcctt ttgaagtacc
    27601 tactacatcg acaaaagtac ttccgtcaaa gatttcaact gtgataacat cacccaattt
    27661 aaaatctcct tcaaatccct tgaactcaac caagtgtctt tgtggcgcta ctcctgcaac
    27721 tttaaaatgt cctgcttccg gcttgttggt atgttttggt ttcttttctt caaaacctac
    27781 ctgaacagct tcataaccgt cggtttcaat gcttttaatt tgagtaacaa cacaaggacc
    27841 agcttcgata acagtgcatg gaacattttt tccatcagca ctgaaaacgg atgtcattcc
    27901 gatttttttc cctattaatc ctggcatttc aattgtttgt ttttttctgt ctttcgtaaa
    27961 cgagagacag aaagttgata gcttatttta taattaaata tttttcaaat ttcctgatct
    28021 ttgaggttta taaccctcaa attttgtcag actttaattt caacttcaac tccgctgggt
    28081 aattccaact tcatcagtgc atctaccgtt ttgctggtgg aactgtaaat atcgatcaac
    28141 cttttgtagg atgacaattc aaactgttcg cgcgactttt tgttgacaaa ggtggaacga
    28201 tttaccgtaa aaattctttt atgggtggga agcggaattg gaccacttac tactgcacct
    28261 gttgccttca ccgtttttac gattttttcg gctgacttgt caaccagatt gtaatcgtat
    28321 gatttcaatt taattctaat tttttgactc atcgctgatg attttgtttt agtgtgttta
    28381 atttttaatt aagagtcatt tactaattaa aaatgtattc attaataatg taataattca
    28441 acttttcctt ttgcttcagc cactacttcc cttgctaacg aattggagac ttccgcataa
    28501 tgtgaaaatt ccattgaaga agttgcacga ccggatgtta tggttcgaag tgcggtaaca
    28561 taaccgaaca tttctgccaa aggaactttt gcctttataa tacgtgctcc ggttcggctc
    28621 gattccatgc cttcaacctg tcctctccgc ttattcaagt cggcaattac gtcgcccatg
    28681 ctttcttctg gagtaaccac ttcaattttc atgataggtt ccaataaaac cggtttggct
    28741 ttcgtgcagg cattcttaaa tgcaatctga gcacaaattt caaacgaaag ttgatcagaa
    28801 tcgacaggat ggaacgagcc atccaatacc gtaaccttta agcggtcgac agggaatcct
    28861 gctaaaactc catttttcat agccgactgg aaacctttct ggatggaagg aatatattct
    28921 ttgggaatat ttccgccctt taccatatct ataaattgca aacttccttc aaaatcgtta
    28981 tcggcaggct ccacgcgaac aataatatcg gcaaatttac cgcgtccacc ggtctgcttt
    29041 ttatacactt cgcgtatttc tacaggctgt gtaatggctt ccttgtaggc aacctgtggc
    29101 cttccctgat tgcattcaac cttaaactca cgtcgcaatc ggtcgataat aatttccaaa
    29161 tgaagttcac ccattccact aataatggtc tgacctgatt gctcatcggt atgtacagtg
    29221 aacgttggat cttcttctgc caattttgac aatcctacgc caagcttatc gagatctttt
    29281 tgtgttttcg gttcaatgct gattccgata actggttcgg ggaaatccat cgattccagc
    29341 acaatgggat ggttttcatc gcaaagggta tctcctgttt taatatcctt aaatccgaca
    29401 ccggctccaa tatctcctgc ggaaatcata tctacaggat tctgtttgtt ggaatgcatt
    29461 tggaaaatac gggaaattct ttccttttta tctgttcgcg gattgtatac gtaagaaccc
    29521 gactcaagtt ttccagaata aacacggaaa tagcacaaac gtcccacata agggtcggtg
    29581 gcaattttga aagccaatgc acacagaggt tcattggggt cgggatgacg aaccactgtt
    29641 ttatcttcgt aacgcggatc ttttccaacg atttcgggat tgtcgagtgg actcggaagg
    29701 taagcacaaa ctgcatcaag catggtttgt attcccttat ttttaaatgc agaaccgcaa
    29761 ataaccggat taatttcctt tgctaatgtt cctttacgaa tagctgcctt aatttcatct
    29821 tcagttattt tagaaggatc atcaaagaat ttctccatca ctgcatcatc atattcagct
    29881 acaatttcca gcattttttc tcgccattcg cgggcatcat tcaccaaatc ggctggaatt
    29941 tcttcttccg aatattcagc tcccattgtt tcgtcgtgcc agaaaatggc tttcattttt
    30001 atcaagtcca ccacaccttt gaatttctct tcagcgccta tggggatttg aattgggcag
    30061 ggtttagcac ccaagacttc tctgatttgg cgaaccactt caaagaaatc ggctcccgag
    30121 cgatccattt tattgacaaa acataaacga ggaacattat atttgtcggc ttgccgccaa
    30181 actgtttccg attgtggttc aacaccacca acggcacaaa aagttgcaac ggctccgtcg
    30241 agaattcgaa gcgaacgttc tacttcaaca gtgaagtcaa cgtgccccgg ggtgtcaatc
    30301 aaatttattt tatattgatg ctgattgtaa ttccaataag tagtagttgc agcggatgta
    30361 atggtaatgc ctcgttcctg ttcctgttcc atccagtcca tagtggcagc gccatcatga
    30421 acttctccga ttttatgtgt taaaccggtg taaaacaaga ttcgttcgga ggtagtggtt
    30481 ttcccggcat cgatgtgagc catgatgcca atatttcttg tataacttaa atctactttc
    30541 gccattttaa aaaaattcct tcaattaaaa tttaaagtgt gcaaacgcac gattagcttc
    30601 cgccatacga tgaatatctt ctttgcgttt ataagctcca ccctgactgt tgaaagcatc
    30661 cacgatttcg gcagccagct tgtcggacat ggaatgccct cctcgtttac gagcatatag
    30721 aattaaattt ttcatcgaaa tggattcttt ccgttcagga cggatttctg tgggaacctg
    30781 gaaagtagct ccaccaacac ggcgagacct aacttcgacc aaaggtgtta cattttcaag
    30841 tgcttttttc cagatttcaa gagaggattt ctcttcgttg gggagtttgt tttttacatt
    30901 ctcgagtgca gaataaaaaa tatcgaaagc aatggatttt ttgccatcga acataagatg
    30961 gttgacaaat tttgttacca tcggatcatt gaaaatcgga tcaggtaata cgacccttct
    31021 tttaggtttc gattttctca ttttttatta aaaatttccg tttttgtttt tagttgcttt
    31081 cttagtatgc ttcaacgact tctttaagtc atttactcaa ctatcatctg cttcctgaaa
    31141 aactcaaaca agtgtttaaa cttgaattga ttttatatat tcaaaaaaat ataaccttat
    31201 tatttaaaaa acttcacttc tgaagtttaa ttttattttt tcttgcctgt tgatgcagct
    31261 gcgggttgtc cttttttagg acgtttggct ccgtattttg aacgacgctg cagacgacca
    31321 ttcactcccg ccgtgtcgag cgtacctcta acaatgtgat aacgaacacc cggcaaatct
    31381 tttacacgac ctccgcgaac cagtacaata gagtgttcct gcaaattgtg tccctcgccc
    31441 ggtatatagg catttacttc cttctgattt gtcagacgca cacgagcaac cttgcgcaaa
    31501 gcagaatttg gtttcttggg cgtagtagtg taaacacgga tacatactcc tctgcgttgt
    31561 ggacatgaat ccaaagcagg tgatttactt ttatcaacga gttctgtcct tccttttcta
    31621 actaattgtt gaattgtagg cattttatta ttagttgtat aaatattttt gagttcttga
    31681 tttcttattc aaacgggttg caaagttagg aattattttt ttatttacaa tgcaatcctc
    31741 aatatttttt taaaaattca ttatctattt gcttttctgc ctcttaaaag cattcgaccc
    31801 gaaaagaatt tggcttagtg agtccggaat ttccgcaaaa aaaccttctt gcggcaaaaa
    31861 agagaatcgg tttttcaatt gtttataaca aaaacgatga ttttagaaat gagaaattat
    31921 ttcagcacgt gaaaaaattt tttctgttac attctttccg gaacttctat acccagcaat
    31981 gccataccat ccttaatcac gtcagcaata gccgaagaaa gaaccaatct gaaattgcgc
    32041 aaatcggtat tttcttcttt caaaatggaa taatcgtgat aaaactgatt gtattctttt
    32101 actaactcat aaaggaaatt ggctatcagg gcaggattaa attcatcacc tgcttccttc
    32161 accattagag gaaattcggt aagaagttga acaagatagc gttccttttg agagatggaa
    32221 aggttcaagt tattcaattt accaaaagaa attgactgtt cttgagcttt tcgcaaaacc
    32281 gaacgaatcc gtgcatgggt atattgaatg aaaggccctg tatttccatt gaaatctatc
    32341 gattcttttg gattaaaagt catgttcttt cgtggatcca ctttcaatat aaaatatttt
    32401 aaggcaccca atgctaccat acgaacgata ttttcaactt cttcgggaga acaaccatca
    32461 agttttccca gttcttcaga agtttcacgc gctgttcgaa gcatttcatc aattaaatca
    32521 tctgcatcta ccaccgtacc ttcacgactt ttcatttttc cgttcggcaa ttcaaccata
    32581 ccgtaggaaa aatgtaccag actttttccc cattcaaatc ccaacttgtc gagcaaaatg
    32641 gaaagaacct gaaaatgata attttgttca ttccctacca cataaaccat tttcttgata
    32701 ggaaaatcat cataacgaac ttttgccgtg ccaatgtcct gagtcatata cactgaagtg
    32761 ccatcagctc tcagcagtaa tttttcgtcc agaccgtcat cccttaaatc agcccaaacc
    32821 gaaccatcgg gacgacgata aaaaatgcct ttgcccaagc cctcttcaac tatttttttc
    32881 ccaacaagat aggtttcgga ttcgtaataa attttatcaa aatcaattcc caatttacga
    32941 taggtttcat taaaaccttc atagacccaa ccattcattg tttcccaaag tttacgaact
    33001 tcaggatcgt tgttttccca tgccaaaagc attttctgag cttcctgcat caacggagcc
    33061 gattttttgg cttcctcttc gctccatcct tcttcgacga gttccctgac ttgttgctta
    33121 tacgcctgat cgaacaacac ataatatttc cccaccagat gatcaccctt taaacctgaa
    33181 ctttgaggcg tttcgccatt accaaacaat ttccaagcaa gcatggactt acaaatgtga
    33241 atgcctcgat cgtttaccaa gtttgtttta acaactttcc atccattggc ttcctgaatt
    33301 tgagcaatac tccatcccag caaattgttg cgtacatgtc ccagatgcaa gggcttgttg
    33361 gtgttgggtg aagaatattc aatcatcatc aagtcggcat tttctttcag cttaactttc
    33421 caaaaatcct tctgtagaaa tatctcattc aacctttcga gccaatatga aacactaatt
    33481 ttcagattca gataaccttt caccacatta taatcttcaa tcacggatga actcttcaac
    33541 aaattatcgc caatttcttt ccctatctgt tcaggagact tgcgtaataa cttgatgaac
    33601 ggaaaaagca caaccgtcat atcgccttca aattcctttc gcgttttctg aacttgaaga
    33661 ggattttgac cgatttccaa cccatataat tgttgtaaag cttgttcaac cagctgttga
    33721 atggtgtgtt ctaaattcat ttccaattgt tttgaaattt ggggcacaaa gttacaaaaa
    33781 aaaatgcatg ttaagatggc gttagactaa tgaagcaaga taaaaagctt ttaccaaaat
    33841 gatgaaaaaa actatttcaa catgagaaaa aatgaaaaac aagcaaactt ccatttaaag
    33901 atattcaaaa agcttactca aataaaaaac gcacaaacaa atgcaacaat ttgcataaat
    33961 ttgtgcgatt cctaataaag taattgaaaa ttaatgaatt ttgtggagct ggagggagtt
    34021 gaaccctcgt ccaaacatgg aaacaatatg ctttctacat gcttagcttc gcttggtttt
    34081 tcgtgtacag gcaaggccga agctaccaac ctgtacctta tcctctatgg ttttcattgc
    34141 atttacgagg ctaaatgcaa ctatcctcga ttttgctgca cctccgtatc aagctgcctc
    34201 gaggagaggg cgcttggaag atgtctcgtc cccgcacctt gtgcagggat aaagcttaat
    34261 ctactatact tcgatcaagc agcgagagcg taattagttt cgccagttaa ttttttgaga
    34321 ttcgggatta acgagccgta aactcagcgc tcgacatgct tacatacctt ttctacatgt
    34381 tgtcaaatcc atgcagcccc ataatttcaa agatccttac ttggcatagc ttactgctct
    34441 tgtttctctg ataacagtaa ttttaacctg accgggatat gtcatttcat cctgtatgcg
    34501 ttttgctata tcgaatgaaa gcaattctgc ttccttatca tctattttat cggcaccgac
    34561 aatcacccgc aattctcttc ctgcctgaat ggcataagtt ttcagaacgc ccggataaga
    34621 aagtgccaaa tcttccaaat ccttcaatcg ctttatgtaa gcttctacaa tttcgcgacg
    34681 agctccggga cgagcgcccg atatggcatc gcatacctga acgataggag ctatgagcga
    34741 ttccatttcg atttcgtcgt gatgggctcc aatggcgttg cagatatcgg gtttttcctt
    34801 gtatttttca gcaagtttca tgccaagtat tgcatgtggc aattccggct catcgtccgg
    34861 aactttacct atgtcatgca gcaatccggc tcttttcgct ttttttacat tcaagcccaa
    34921 ctcggcagcc attgttgcac acaaattggc tgtttctcgt gaatgttgca acaaattttg
    34981 accataggaa gaacgatatt tcatttttcc caccaaacga atcagttcag gatgaagtcc
    35041 atgaatcccc aaatcgatgg tggtacgttt ccctacttca ataatttctt cttccagctg
    35101 cttacgcacc ttgttgacca cttcttcaat gcgtgccgga tgaatacgtc catcggtaac
    35161 caactgatga agagaaagcc gtgcaatctc gcgtcgaacg ggatcaaaag ccgacagcac
    35221 aatggcttcg ggcgtatcgt ccacaatgat ttcaactccg gtggcagctt caagtgcacg
    35281 aatatttctt ccctcgcgcc caataatgcg acctttgatt tcatcggact ctatatggaa
    35341 caccgtaacg gaattttcga tggccgtttc agtagccaac cgttgaatgc tctgaatgac
    35401 aattcttttg gcttccttgt tggcagtcat cttggcttca tccataatat cgttgacata
    35461 agccatagct tccgttttgg cttcttcttt cagcgattcg ataagccgtt ctttagcctg
    35521 ctcggaagaa agaccagaaa cagtttccaa ttgttcctga gcctgacgga tcaaatgttc
    35581 gagttccttt ttcttgtttt cgacagttaa aaactgactt tccaagttct ctttcatcac
    35641 atccacttca ttcatttttc gctgcaattc tccctgtttt tggttgagtt gcatttctcg
    35701 ctgttgaatt cgggtctcgg ctgcctgaat tttggcattt ctttgctgta cctgttgttc
    35761 gtgttcggct ttcagcgcaa taaacttttc ctttacttca atcaggttgt tctttttgat
    35821 caactccgct tcggcttcga gttctttcaa agctttttta tgattgcgtt tgaagtaaat
    35881 gaaactaccg cctacaccaa caccaaaaga tattataatg gcaataatta gattcatttc
    35941 gtatatatat ttatttgata aacaattaat tacaatttca atttgataat aaaaaaacgc
    36001 attaacgttg acaacttcgg gttaaaattt ttttaaccaa ctaaacaacg taatgcgaga
    36061 aaaattgttt gaagaacgat tcttcgaaaa ttacttttca gccaataatt tttccagttc
    36121 ctgattcaga gaagacactt tttctgccag aggcgcagta tcctgctgca tcatttttct
    36181 gacaagcatt actgcaagct cgtaggcaac aaaaataaaa acttcttctg ccgaccggtg
    36241 actgtatttc ttttgataaa cttccagata ttttcccaaa actttttctg ccaatcgata
    36301 caactcttct tctttgcgtt caattctcaa tggcaacctg taaccgccta tctgtacatt
    36361 tattaaaaaa gtatcgtttt cgttcataac cgctctaatc atttaacaaa agagccaaac
    36421 acttatcaat ttcccgcacc agtttgttga tttgctgctt tgacttgatc cgatcttcat
    36481 cagaaaggga caaagtttta gcaattcgca aatggtcata atttttttga agctcggaaa
    36541 acttgacttt taagagttca atatcttgtt ctttattgct caactgcgct cttaacaacg
    36601 aattttcctc ctgcaatgac ttgaaatcct ccatcagctt tcgcagattg gattcaaaag
    36661 ttgcgaccaa atcatcataa gaatgcgtca ttttatcaaa aaaatctata caaaaataca
    36721 tttttcagtc gaaattcata tacgaaatta cctttttttt cgtatattga taataattat
    36781 tgtttttaaa ttatttttaa taaatgtatt tgtgctctaa attgtttaaa taacaggatt
    36841 cgagaataaa taatccgtat ttttgcaaaa aatttcgtaa catcatgtgg aaaatagatc
    36901 ctttcaatta tcagcgtcgc cccagcaccg aagtccgcat cggccaactt ttgatgggag
    36961 gcactcaacc gatcagaatt caatcgatga ccaataccga cacaaataac atagaagcat
    37021 cgttagagca atgcatccga ctgattcgtg ccggcagtga actggttcgc tttaccactc
    37081 aaggaaacag agaagcggaa agtttaaaaa aaattcacga tttattgcgt gataaagggt
    37141 atcaaactcc gttgtcggcc gatgtgcatt ttaacgccaa tgtggccgat gtggtggctc
    37201 aatatgtaga aaaagtccgt atcaatcccg gtaattatct gggaagcaac agaggaaaca
    37261 ttacttccga ttatagtgat gaagaatata aagctgaata tgaaaggata agaaaacgct
    37321 ttaaacagtt actgaatatt tgtaataaac atcaaacggc gcttcgtatt ggtgtcaatc
    37381 atggttcttt gagcgaaaga attatgaatc ggtatggaga tacggctgaa ggcatggtag
    37441 aatcatgcat ggaatttctt cggatttgca gagaagaaaa ttttcacaac gtggtgattt
    37501 ccatgaaagc atccaacacc cttaccatga ttcattcggt tagaatgctg gtggaaagga
    37561 tggaagctga aaattttcat ttccctttgc atttgggcgt tacggaagca ggcaatgaag
    37621 aggaaggccg aatcaaatcg gcagtgggaa ttggcaccct tctttccgaa ggaattggag
    37681 ataccataag ggtttcgctg agcgaagacc cggaagcaga aattcctgta gctcgaaaac
    37741 tgatttctta tattgacgca aaaaaaaatc acgaaccaat aattgccgag aaaaacttcc
    37801 aacattctcc tttcaattat catcgacgga caacacatgc cgtagaaaat ataggaggaa
    37861 ataatgttcc tgtggttata gctcacacaa aagaaaattt tgagattact cccgattatt
    37921 atttcgccga aaatcagata ataaacatcc acaatgaagt ttttcccatt tttgacatcg
    37981 atgaaaaaga aaaaataata aaacattcct cccgcatcaa gttcctaaaa atgtcgttcc
    38041 ccgaactgac aaaagaaaat gtgcattttc ttaaaaacac ggccgataca gtggttattc
    38101 tcgaaagcca tcataaaaat gcaccggctg aacaaaaagc tttttttcat tcacttatga
    38161 atgccggttg cacagttccc gtaatcgtta cgcaaagcta taaagaaaac atagaggaag
    38221 acttggaaat caaagccggg gttgattttg gtatgttatt tgttgaggga ttcggcgatg
    38281 gaattttttt aaaaaatgaa ggaaccatcc cgtccgaaaa acttaattcc atagcgtttg
    38341 gaattttgca ggcaacaagg gcaagaatct caaaaaccga atttatttcc tgtcccggtt
    38401 gcggtagaac aatgttcgac cttcaaaccg ttgtttcccg aataaagtca aaaacttctc
    38461 acttaaaagg attaaaaatc ggcattatgg gttgcattgt caatggaata ggagaaatgg
    38521 ccgatgccga ttacggatac gttggagcag ggcgaggtaa agttagttta tacaagaaaa
    38581 agatttgtgt tgaaaaaaat attcctgaag aagaagcggt ggaaaaattg atcgaactca
    38641 ttaaaataaa tggcgactgg acagaaccta tttcttaatt ctgtgcttcg caattttttc
    38701 tgtatttatt atcaaccaaa aaatgaaaaa tatgaaagaa aaattaattg ttttgttact
    38761 tgcttcggca tcttttatgg gattaaatgc tcaggtcaga ggtcaggcgc ttggtgtgag
    38821 actgggatac ggaggggaag tttcctatca gcatcccttg aacagtcaga acagaattga
    38881 aggtgatttg ggattttact acgatcacgg attcaatttg gcaggagttt atcaatgggt
    38941 ttttgacttg tcggaacttt ctcccggttt taactggtat gttggcctgg gaccgcaaat
    39001 tggcagttgg tcaaaacata acaataaaga ctatgatgga ctggcattgg gaattgttgg
    39061 acaaatcggt attgaattca atttcaatat tcccttgcaa ctttcattgg attatcgtcc
    39121 cggctggtac gtactgcctt ccagttacgg aggagcatgg gaaggtattg cgctgggggt
    39181 gagatataat ttctaatcaa acgattcata aaaaagaaag acagatggga aaagtttcct
    39241 gtctgtcttt tttgtttcat gatcgataaa aaagcacatc gaatattcaa cgtgctttcc
    39301 aaaattaagt tgactatttt ttctaattat tttgcaggta caacaggtaa tttttgagga
    39361 gccttctgct gatttccgtt attttgctgg ccgccattat tgtcttgctg attgttacct
    39421 gctccttgag tttcaccaaa atcgggagct acaacgcccg gtaattcttc ctgggctttt
    39481 tgatactgtt ctttgatttc ggaatcgctt cctccggatt tctgattggc gtgaatgccg
    39541 gtggcaccaa tggaaagcac cacaatgaaa gcaattaatc cccatgtagc tttttccaga
    39601 aaatcggctg ttttgcgaac tcccatgtat tgattggaag aagaaaaacc cgctgccagt
    39661 ccacctcctt ttgaattttg taccaacaca ataagtatca ggagaatgga ggcaattata
    39721 attaaaatag taaacaaaag gtacataaaa gtatattatt taaattttca agttttctat
    39781 tactttttcc aagaaacgaa tttggtctgc aaagtaaaca ctttttttcg gattattcaa
    39841 atttaatttt tttaaaattt ccaatgcttc agtgtatttt ctgttcttaa ttaacctttt
    39901 aaccgattct tctgaaattt caagctcagg aatgatttct tccgcaaaat attccgattg
    39961 gagtgcggac gattttgtag accctttctt cttaatgggt tcttcttcat ctggtgccgg
    40021 tggattttcc atcaccatag ctcgcgcttt tttcaactga tcggccaaat ttttcaaagt
    40081 ctgacctgaa tctcctcctt ccttttcaac tacatccaat aagtcgaaat aagaacctgt
    40141 aagttttcct ttttcgggac gacgataaac ttcctttgca aaaacttccg gataaagaaa
    40201 atgatagagc cagcgacgtt gagaagtata gacaactgca gttttaatgg ttttttcaaa
    40261 atgaatacta cccgacactt gaagagcttt cagataaagc atccttgcct gcgaaaaata
    40321 gggataatgc tctaccaaat cttttagatc aggagtatgt tccgatgtaa tgctttcggg
    40381 atgctgcaaa agtgttaaaa attctgcctg tgtcattatt cctaaatgct caaaaaaacc
    40441 agaaaaaaca tttccttacc atttagcaac agtgtcgtta taaatatttt cagtaatctc
    40501 ggtaataatc gccttcacca actcttcctg cacatcattc aacatttttg agctgtcgaa
    40561 aacctgaaaa gccgaatagg ttttttcaaa atcatctgcc ggattcacct tattggtaaa
    40621 acgaacctta acggttaccg tcagtttggt ttcggaagca taggtgtcgg cactaatgga
    40681 catgggcgta agctgatagc cgacaatctc gccttccaaa ttcaaatctc cattttgacg
    40741 gacaagccga agttgggtct gcttggtgta aatatcgcgg agcgcttcgg aaaactgctg
    40801 cgacaaaggc ggataaacca actccgaaac atttggaaag tcggcaatgg aaatagtttt
    40861 gattttagtg taatcgatag aagctccatt gaatttgtat gaaacggtac aggaagccaa
    40921 aagaagaaaa gttccacaaa aaattgccga taatagtttg accgatttcg tgtttcttcc
    40981 aaaaaatgag tttctcggtt ttctattcaa ttccatattc ttttattttg cggtacaggg
    41041 ttcgttctga aattttcaat tcttcagcag ctgcttttct tttgccctta tgcttttcga
    41101 gcgtcttctt tatcatttgg cgttccatat cttcgagcgt cattggttct tgtatatctt
    41161 caatctcttg gaattcagca gcttcttcaa aagaattttg tttgtttgta ttgggaaact
    41221 gatttacttc gcgccccaaa ttattgacag gtttcatcac gtccaccggc accggtgtag
    41281 ccggaacaaa agtctcatgc ggagcaacat aagaagacga taaagggaag tctttttgta
    41341 gcagttcctg attgccctga gcattcatta aattgtaaac cagttttttc agttctatca
    41401 tatcctgttt catgtcgaaa agcacctgat aaaggatttc tctctcggaa gtgaaagttt
    41461 tctgatcgct tcgatgattg gacaacaaaa caggaaaacg ctccaattca tccttcggca
    41521 gatattgttt caaaatttct gctgtaattt cccgttgctg ctcaatgaca gaaatttgtt
    41581 cggtaatatt tttaagttgc cgaacattac cattccaata atattcggtg agcagttttt
    41641 ttgcatcttc cgtaagttta atgggtggca tgtggtattt ttcggcaaaa tcggctgcaa
    41701 attttctgaa caacagaaca atatcttctt ttctttccct caaaggcggg acataaatgg
    41761 gaacggtatt taaacgataa tacaaatctt cccgaaaacg tccgtcccta actgcctgcg
    41821 tcatgttcaa attggtggca gcaataacac gaacattggt tttctgaatt tttgaagaac
    41881 caaccttgat aaattcacct gtttcgagca cacgcaataa gcgaacctga gtggaaagcg
    41941 gtaactcacc cacttcatcc aaaaaaatag tacctccgtc tgctacttca aaatagccct
    42001 ttcggtcggc catagctccg gtgaatgaac ctttttcgtg cccaaacagt tccgaatcaa
    42061 tcgttccttc aggtatggcg ccacaattga cggcaatata aggtccgtgt ttgcgatgac
    42121 tgtaataatg aataatttga ggaaaatttt ccttgccaac gccgctttcg ccggtaatca
    42181 ataccgataa atccgttggc gccacctgaa cggccacgtc gatagcccga tttaacaatt
    42241 cagaatttcc aataattcca aaacgttgtt ttacagcttg tatttctgat ggttgcatgt
    42301 ttctaacaat aatttactgc aataatgaca taattgtctg acaaaattaa tgaaaataaa
    42361 aggattttgt ttattccaca tcatgaaaaa gtctaataaa agaatttatt tcccaatttt
    42421 tgattaccga atttgaaaaa tgttgttttt tggaattgag aaaaagaaat ttaaaacgaa
    42481 gaaaacaaat tattggaaaa aattttgaag gtaaaaaaat ttgtgtaatt ttggtcaacc
    42541 aattaaacgg gttaaaaaat aattttaaaa tttttccttt ttttcggcag agaaatattc
    42601 ggaacaaata agtcaataga aaaaactaca aatattatga tggaagataa ttttatccga
    42661 ttgatgaaag cccagcaaaa atcgcctgca gatgaagtaa tcaataaaat taaagaatta
    42721 attgagtcag gtgaattaaa acccggagat cgattgcctg ccgaaaggaa attggcaagc
    42781 gactttggtt ttgggcggac acacattcgc gaagcattac aaaaactcga attttatggc
    42841 atcgtaaaaa cacttccaca gagtggaact attctcaatg ggctggatat aaattcatta
    42901 aacggcttgc tttctgatgt tttaaatctt cagagttacg atttttattc gttggttgaa
    42961 accagatgca tcctcgaaat gaatgccatt cggctatgtg ccgaaagacg taccgaagaa
    43021 gacattgagg caatggaaaa atctcatcag aattttatca aattccataa tgatgaaatt
    43081 ttacgtgtca gttacgactt tgcttttcac cgcaacatag cagaaggcag tcacaatccg
    43141 gtcatcaaat ccatgcttat gaccattatt ccggatattc tcatcatcta caataaagaa
    43201 agggtatgta ataccgaccc ttttgtagta attaaagaac acgccgaaat gatcaatgca
    43261 gtgaaaatgc aaaatggaga attggcctca tccttgcttt accaacatgt gcaaggcgtt
    43321 atgactctcg ctcgcacgaa acttgaaaac ttaaaagtga aaagtttgag tatggcttga
    43381 gatatctaaa ctgaatcgtt aagacttcct tagactcaac ttaaatggaa taaagctgct
    43441 ctcaaattgt taaaaaggca gcttttttta ttctcaacaa gtctgaaaat atgttttgca
    43501 acatatttcg taaaataacc tgaaaaaaat ttttttttat tataaaaaca ccgtattttt
    43561 ggtcgaccaa taattggttg accaattttt ttgtcttata aagaatgaaa tcaaaacaat
    43621 aataatttac tcattttcaa taaaaataca aagatttata gtaaaacaga ttcgtataaa
    43681 accataaaaa catgaaaaga acaaaaaaac caaaattctt aaagagagaa gttcttgtat
    43741 taatagcatt atttattagt acaatcagtg cattttctca aattaaagtt actggaacag
    43801 tgttggacac taaaagagaa ccattaattg gtgtcaatat tactgtaaaa ggctccagga
    43861 cggggacaat ttcagatttc aatggcaaat tcaacattga cgtccccgat agacaatcag
    43921 tactggtttt ttcttatatt ggctacaaaa caatagaaag aacaatacct gccagtggac
    43981 aacttactgt tattttggaa gaagaaaccc aaaccattga cgaagttgtt gttgtagctt
    44041 atggcactca aaaaaaatct cacttgacag gagctgtctc ttctttaaaa aacgagaaaa
    44101 tggatgaaat tccggtttcc agagtagatc aggcgttaca aggtaaaatt gccggcgttc
    44161 aaatcaataa tgtaaatcca gaagccggtg cagctccaaa aattcgtgtt cgtggcttgg
    44221 gttccatcag tgcaagcagt gatccattga tagtaataga tggatttcca gttcccgatg
    44281 gcttatcgat ggttagcatg gcagatgttc agtctgttga agtgttgaaa gatgcttcct
    44341 ctgcagcatt gtacggttca agagccgcag gaggagtcat actgatcacc acaaaaagtg
    44401 gagaaattaa caaacccaaa tacaatttca aaatgtattc aggttttcgg aatccattga
    44461 aacttccaga catgttaagt actgaagaat atgtggctct tttgtatcgt gaagctgaca
    44521 tgagaaagct ggatcctgcc gttaatggaa tagccaccga gacaatggca tttaataaaa
    44581 taacagaacc tgaaaaggca ggctacctga ttatgaaata tttggatgat caacctaccg
    44641 attggctcaa tgaagggttg agagatcgtg gaaccatgca gaattaccaa ctgagtgctt
    44701 caggaggtga taaaaatttg aaatattttg tttcgggaaa ctacgtaaat gaagatggga
    44761 tcatgttgaa cagtacttat gacaaattta cttttagagc caaattggat gccaagttat
    44821 ctaaagcagc cactttagga gtaaatcttt cacctactta tagccgacaa gaacgacctg
    44881 ctgtggattt aactgattat atgcgattcc cgtcttggct gccaatcagg cataacgaag
    44941 ccaccgctgc cttaaccggt aaagttgctg gagattatgc tcagccaagc gactttaatg
    45001 gcattaatat tagcggtata ggctacaaca atgaggtttg gagcttgccg ggagttacgg
    45061 tttggaattc aagtaatcag aatcctgtct ccatcaggga acgaaccagt atctttactg
    45121 acgactacag atttcagggt aatatttatt tgactttaaa tctattaccc ggattacaat
    45181 tcaaaacttc caatggagca tatcttttat ttagagaata caacaaaaaa gaacaaacct
    45241 ctgccaacaa ggcaggaaat ccaaattccc ttacccgaca actgactacg catagggaat
    45301 tactttctga aaataccctg aactacaata agaaaatcag caatcacgaa tttgatgcct
    45361 tattgggttt caccatgcaa caaaccaatg acaattataa caggattgta ggtaccaatt
    45421 tccccgacga acaaattctt tctttcaatc ttgcttctca aattctattg gacagtcctt
    45481 ctattgatgg aacaacttct ttttattata ccgaatcgct ggtttctctc ttaggccgtc
    45541 ttaattatgc ttatcttggc aaatacatga tgtcagccag ttttagagca gatgggagct
    45601 caaagtttta caaaggtaat aaatggggta cattccccgc attttcagca gggtggcgtg
    45661 cttctgaaga gaaattcatg aagaattttg aatggctatc caatttaaaa ttacgcgtca
    45721 gttatgggtt aacaggaaat aacaacattc ctcaatatgc ttacatgaac agtatcaaca
    45781 ccagcaacta tgtgctgggt tcaggaaatg gaacgttggt acaaggaatg gtttctaaca
    45841 gttcttcgtt agggaatccc gatattactt gggaacaaac atccgaagcc aattacggaa
    45901 tagattttgg ctttttgaac agcagactga acttttcgat agaatattat aattccaata
    45961 cgattcaatt attactacaa caacctgcca tgtatattac cgggcatcaa tcttattgga
    46021 acaatattgg gaaggtaaac aacaaaggaa tagaaataga gctcaatacg accaatatcg
    46081 ccaataaaaa atttacatgg aaaacttcag cgaatttttc caccaacaaa aataagctct
    46141 taaattacgg agacaaggaa tatgaagata atttcggaga aaggagtgaa gtatatcgtg
    46201 ccatagtggg agagccggcc attcagtatt acggttataa aaccgatggc gtatatacaa
    46261 cttttgaaga agtagcagca gcaaaggcct taaaagataa aaacggcaat ccgtttactt
    46321 acaccaaatt tgcacctatt gtaggaggaa ttaaagtagt gaatatcaat ggcgacaata
    46381 aaattgatac cgaagaccgt actgttttag gagatccatt ccctgatttt acatggggaa
    46441 taaccaatac gtttacttat aaaaactttg atttaagttt tttgattcaa ggcgttcaag
    46501 gtattgacat tatcaatggc aatgtaaatt ataatgaaca actgcgctac aacaaggctt
    46561 acaccaacaa tcgatatgtg agtcccatgt ttccaggaga tggaaaaacc gtttattcca
    46621 atactacttc tggtagcgat ttgatgttga ctgactactg catagaagat ggttcttatg
    46681 gcgcaattcg tgatttcact ttaggatata ctttacctca aaacttattg aaatcattga
    46741 aattaagaaa tttaagagta tatttttctg ccgagaattt ggtttatatc atggcaccga
    46801 gttatagagg tattaatcct gaagcaagga gaacagctag caattacact agcccattgg
    46861 tagacggtta tcaacgtggt gctttccctt tgaacagaac ttttacggca ggattggata
    46921 ttaattttta attaaggagc tttttattat gaccatacta aataaaaata caaatatgaa
    46981 aaataaaata tttattacag caatttcgct gtgtggattc tttttgacaa gttgtgaaga
    47041 cttccttacg gacaagcctg agtcagtact tacacaggtt gatttctata caacccctat
    47101 acgaatcaat cagggagtaa tcggctgcta tgctggcctc gcaaatatac agaaagatga
    47161 atggatgtat accgaactca ggagcgacaa ttcatgtcaa agtgaaacag gctcgagtgc
    47221 aacaatcaga attgatcaaa ccgatatagc ttcttttagg ctttctccaa gtttacctgc
    47281 tttgcgcgat tactggtata aaattttcca gaatatttcc aatattaacg cagtgctacc
    47341 ttctgtaaga gacaatagtt atgttgcaaa tgaatcactc agagcacaat atgaaggtga
    47401 acttttgttt ttgagagctt atcattatta cacgctggtc aatttgtttg gcgatatgtt
    47461 taaaataacc aaagttatcg ggccaaatga agcaaaaaaa attcctcgtt cgccggtttc
    47521 tgaaatctat aatgaaataa ttattcccga tttgaaaaag gcagttgaag aattaccttc
    47581 cacctactca gcaacagact tgggaagagc caccaagtgg gcagccaaag gaatgttggc
    47641 caaggcttac atgatgttgg gcggaaagga taatttatca ttggctaaaa aagaattgga
    47701 agacattcta acactatcgc cacatggttt gttaactggt acaggtgctt atgcaaatat
    47761 tttcagtatt agcaacgaaa tgaacaagga aataattttt gccgtacgat acaaaggcgg
    47821 tagttccggc attggttcac cattctgggg cacttttgca cctttgggtt ctgcaaactt
    47881 attcttaaaa atcggaacac cgcttggtta taataatccc acttatgagt tgatgaactt
    47941 gttcaatgca aatccaaatg atactcgtaa ggatgcttgt ttcaaagtat ggaataaatc
    48001 gtcaactgtt tcagtgcctt atatttccaa atatatggat gcatctatga cccaaccgct
    48061 tcaaagtgaa aatgactgga ttattttacg ttttgccgac ataaaattgc tttatgccga
    48121 aattttggct caggacggga attttgcaaa tgcgcacaat ccgcttaatg atgtcagagc
    48181 aagagccgga ctagatggaa ttggtccttt tggttcaaaa gaagaagctt tagatgctgt
    48241 atatgccgaa agacgcttgg agcttgcatt tgagaatcag cgctggtttg atttgcttcg
    48301 catggctaaa tcgtatgaca atccggacaa accgatggag attttaagga gacatacctt
    48361 tgaaacagat tgggcagtgc tttattcaaa atacaatccc atgcaaccgc ctgaagaaag
    48421 attttatatc aaggaaaggt tgttgctccc gattccacaa gctgaaatcg acaccaataa
    48481 tgaaatggta attcctcaaa atccaaccta ttaaaaattt tcaaacaaaa attttatcgt
    48541 catgaaaaat ataaagaaat tttccttatt ctttttattc atcggattaa ttggagcatc
    48601 gtgttcaacc gacgaagaaa acacagtagt tccaaaaaca ttggaacaat acaaacagga
    48661 tttcagtcaa tttgtgtcgg cagaaaaaac taaagttcaa aattgtaaaa tgggttacaa
    48721 taaaggagat tttaaatact cgagcgattc aacaaatttc aaaacatatt gtgcagatta
    48781 tttgaatgtc ttgattgctg cagaagcagt tttagcaaaa tcagatttaa ctatagctga
    48841 tattgttaag gcaaatggaa ctttagcatc gccaggtaaa ttatttaaca gcagtttatg
    48901 gatctctgac agacgtcctc tgaatgatgc aataattgca gctcaagagt tattagctgc
    48961 tacacctgaa ggtactgatc ccggacaagc accagcagaa gcaagaacag cttttaccaa
    49021 tgccattaca gcagccaaaa aagttagaga ttcgtcttat acaattgagc gacaagtaca
    49081 agaggcggtt caaaaattgg aagaagctaa aaatgccttt ttggcagcca ttattcatta
    49141 attttccatc ataactattt aaatttaatg aaaaaaacat tcggcatttt attattcatg
    49201 tttctcccat tttcggtgat attgggaaca agttataaag taatgaactt ggatcaattt
    49261 aatgaaaaat ccaaattgct actacccggc gacagcatca ttttgagcaa tggagtttgg
    49321 aaagatgctc aattggtatt caagggaaat ggggagaaaa acaaatacat ttatcttctg
    49381 gctgaaaatt cggggaaagt cagtttggaa ggagaatcgt gcttaagaat gtcgggaaac
    49441 tggttgtatg tttccggtct tgtatttcga aatggacaca ctccaagaaa gacggtaatt
    49501 gagtttagaa ctggctccaa agaatatgcc tttaattcag ttttagccga atgtgtcgtt
    49561 gaccaattca accaaaaagt gaaagattca accgaccatt ggattgccct ttatggacaa
    49621 aaaaacacga taagaaattg ttattttgcc ggcaaaacaa atctgggaac cactttaatc
    49681 gtttgtcccg atgacgaaaa ctccatcaac aatcaacatt tgatttatag aaattacttc
    49741 ggttttcgac ctcgtctcgg ttccaatggt ggagaaacca ttcgtattgg taccagtgaa
    49801 gtttgcacca acagttcaga gacaatagta aaagggaatt attttgaaca ttgcagtgga
    49861 gaaacggaaa ttatatccaa caaatctaca gataattcat tccttaataa tactttttac
    49921 gaatgcgaag gtagtttagt gcttcgtcac ggaaacaatg ctacagtagc aggtaattgg
    49981 tttattggta atggtaaagc ttttaccgga ggaataaggg ttatcaatga aggacataaa
    50041 attttcaata attattttta tcgtttggct ggagatgatt tccgttcgag tttggctatt
    50101 atgaatggca ttcctaattc accagccaat ggttatgctc ctgtaaaaaa tgtaatagtt
    50161 gccaataata cgtattatga ttgtacatct ccttggtcat ttggagtagg atttggattt
    50221 cgaaaccgca ttgcaaaacc tgaaaatacc ttgttattga ataatttggt ttattgccca
    50281 aataccgatg aactgattaa atattacgac cagatggacg gtatcatgtt ggacaacaat
    50341 attttggtga atagcaaggg gaacctggaa atgaaaggaa cggtaaaagg tgaaattttt
    50401 caatcaaaaa tgaacgattt agagattgta tataccaata caacagctaa aagacttgct
    50461 tttattaaag aagacatttt gggacaagtt agaacaactc ctgttatcgg cgcttttcag
    50521 aacaatatcg taaatccgac tatagcaatg gcaacaaaac ataattgtgg tcctgaatgg
    50581 tatagaaata agataaccga aactacaaaa acagaaaaac gttttgggaa agccacagtt
    50641 gtagaagcag gacttgattt actttccaaa gctgtaaaaa atgccggcga taacgatacc
    50701 ttgttactga tgccaggaga acatgtagtt acaaaaaaaa tggctattaa ccaaaatttg
    50761 gttattcgct cagctgacaa taaggtaaaa ccaatcataa ttatgaaagc cgacaaggaa
    50821 aatgcttaca tgttcgaaat ctcaaataat gtaaagctac aacttgaagg tgtgaagatt
    50881 gatggtaaca gtgaatcgaa tttcccaacc aaatacgcct ttatttcttc tcgtgaaggt
    50941 gctgcgcatt tttctttaat agtgaatcaa tgcgaaattt ctggttttaa cgtaaaaact
    51001 ggtagcattt atcgcgctta caaaggttca tatgctgact caatagtggt aaccaataca
    51061 gtgatgaaag actgttttcg tggttttagt ctgagcgaag aaaaagatga taaagggaca
    51121 tacaatgcag aaatagtaaa atttgaaaat tcggttttcg acaattttac ccaacacgct
    51181 ttggactatt acaggggagg gaatgacgaa tctactttgg gaggttttct ttttgtaaat
    51241 cattgtgttt ttaacaatat cggccaagac gaaaaacagt ccatgctaaa gctaaacggt
    51301 attgtgaatg tttccattgc caattctatt ttttccaact caatggcaaa gacaagtgtc
    51361 aaactttcag gtaataaaaa cataattcag aactgctgtt tttataattg tccggcacca
    51421 aaagtagaaa acggagctgt ctcacaaaat ctttatttcg acaatccccg atttgagaaa
    51481 aaaagttttc tcctttcaaa gaaatctcct ttaataggga aagctactga cggaggaaat
    51541 attggtttga gataaatttt tgttatttca ggagaaatat tcattatgat aaaagctaaa
    51601 cgagtttcga tttttttctt aatattcatt tttttgtatc ccatttctct ttcagcgaaa
    51661 aatccgctga aagagaaatg ggtgcaaacc gcaattcaaa gatccgaaga acaattgtcg
    51721 gctgctattg tacaattcag ggattccaat aaaacaccca gaacattcga aaacgggaaa
    51781 atagttttag tgtcctacaa agactggaca agtggttttt tccctggaag tttatggtat
    51841 atttacgaac ttactggtaa caaaaaattt gaaaaaactg ctgagcattt taccacttta
    51901 cttaacaaag cacagtatcg tactgatacc cacgacttgg gattcatttt aaattgcagc
    51961 tatggaaatg cctatagaat tacaaaaaac acggattata aaacggttct tatcaacgga
    52021 gcaaattcat taatgaaacg ttatcatcct aaagttggtt gcatcaaatc atgggatttt
    52081 ggcaactggc aatatccagt aattatagat aatatgatga atttggagtt tctgatgaat
    52141 gttgggaaaa tgacagataa ccagccaatg ataaatgcag caatttctca tgcggataaa
    52201 acgatgaaaa atcattttcg ttcagatttt agctgttttc atgtagtgga ttatgacacc
    52261 ttaacaggga aagtgattaa aaaacaaact catcaaggat ttaatgacag ttcatcatgg
    52321 gcaagaggac aatcttgggc gctatatggt tttaccatga tgtttcgtga aacagggaaa
    52381 gttgagtatt tacatcaagc agaaaaaatt gcttcttttt tattaaatca tcctcgatta
    52441 cccaaagaca aaataccgta ttgggatttc gatgcaccca atatcccctt tgaaccacgc
    52501 gatgcttccg ctgctgccat tatggcatct gctttgattg aacttagtac ttttgtaaaa
    52561 gacaaacccg aatattttaa aacagctact atcattctta aaaatttgtc atcagataaa
    52621 tatctatcta aaaaggggaa aaatggtttt tttattctta aacattctac aggaaactat
    52681 cctgcaaaaa gtgaaattga tgttcctctc tcgtatgctg actattattt tttagaagct
    52741 ttgcagcgat accttgaata caagaaataa cttaggaagt tatcgaaaat tttttgttat
    52801 caaattgatt aacaaaatat taatgttaaa ttttgaatta atcttaaaaa tcaaaagaac
    52861 aaaatgaaaa acaaatatct catcattcca acgataatta tatttacttc cttcactcta
    52921 aagtctcaaa ctttattgga agctaacgga ccggggaata cctatgaatt gattaacagc
    52981 atttttgctc caaatggcgg taatgtagta gaaacgcccg attgtgctca tccggaattt
    53041 ggtcgtcata ttgcggaagt ttgggatgaa gatttacaaa aatatgtctt tgaattttat
    53101 attcatgtaa cccctgataa tgaccgttgt ataaattttg accggcaaag ggtagaaatt
    53161 aaaacgtatg aaccatcacc ggaaaattta aaaggaacaa gtggagaaac agtggtttac
    53221 aagtggtttt ttaaaattcc tactggattt caaccttcct ccaatttcac tcatcttcat
    53281 cagataaagc ctgtgggagg agatgaagat acgccaatct ttgctttaac tgcccgaaag
    53341 gggaatccaa acagattgga actaaattat tacgaaagtt ccaatcaaag tgcaatcaaa
    53401 ttaaaaacta tttacctctc atggttagaa aattcttggg tggaagttac agaacgaata
    53461 aaaatcgatc ctgaaaatgg gaattatgcc atcaccatca aaaggattaa tgatggtatt
    53521 gttttattgg attacgaaaa tcaacattta ctaactatcc gacctgataa taattttatt
    53581 cgtcccaaat ggggaattta tcgaagtctt ttgtccagcc aggatttaag ggatgaatcc
    53641 gtacgctttg ccgatttttc gattgaagaa gtaaattcaa cagctttaaa taaaaccatt
    53701 ttggaaaatt ccaatttttc tttttttaac gattcaaaga acaaaaaatt aaaagtcaat
    53761 tttaccttaa accagccttc ggacgtagca tttagttgca tgttacttaa tggccaacgg
    53821 gcaaaagagt tttcctccta taaaaattta ccggttggag agcaccaagc atatttagac
    53881 tttggaaata tgttgccagg tatttatctt ttaagcatga aaacctctta cggagtgata
    53941 tcgaaaaaaa tgcttattca gccttagaaa ttcagaggca ataatataat ttttttgatg
    54001 tctgaaagtc agtagaaaac acaatttttt tttaaaaact tttggaagaa aatcaacatt
    54061 tgaattttca tcaacttacc gcttattatg gaaagtatta caagaagaga attcttaaaa
    54121 agaagtttgt tgacagtcgg gaccctgccg ctgttaggtt taaacgaaaa attgtgggca
    54181 atgagcagta gtcaggaaaa acttccggtt tgcatttttt ccaaacatct tcaatttctc
    54241 gattggaggt cgttgggtga aaaagtaacc gaaatgggat ttgctggcat cgatttgacc
    54301 gttcgtaagg atggacatgt cgaaccggct gctgttgaaa cggatttgcc aaaagctgtg
    54361 gacgagatta aaaagggagg ctcaacttgt gtaatgttat caacaacaat agagaatgtt
    54421 cataacgaac aggacagaaa tgtgatagaa agtgcagcaa aatgtaacat tccattctat
    54481 cgaacgaact ggttcaaata tcctgccgat aaatcgcaac ccgaagcttt ggaaatgtat
    54541 gcttccaatt tgaaagaaat gagtgaattg aatcgaaaac tgggaatcgt gggttgttat
    54601 caaaaccatg ccggaacgct tgtaggcgct tctctttggg aagtttacaa aatgctcgaa
    54661 aaagtggata aggattattt cggcgtacag tacgacattc gtcatgcaat ggtggaaggt
    54721 gccaattcgt ggatcaatgg ttttgaattg attcgatcac aaataaaaac cattgtactg
    54781 aaagattata aatggggaca ggtaaatggc aagtggaaag ttgtcaatac tccgattggt
    54841 gaaggaatgg ttgattttaa aaattatttc aagcttttga aaaaatatcg tatcaacgtt
    54901 cccatctcat tgcattgcga atacgatttg ggaggagcag aaaaaggaaa tagaaaaata
    54961 tcggtttacc ctcaagtagt atttgatgct atgacaaaag atttgaaaac gattcaacaa
    55021 ctttgggaag aagcataaat gatttaacgt taatttaaaa agtaaaactc aaacaaatga
    55081 attttgataa gttgaaaatt ttaacttccg ccatttgctt tttttgcgga tcattccttt
    55141 ttgcacaaca acgaattccg tcagatgtgg ttatttctcc tgaaaatttg gctcaatacc
    55201 tgaatgacag tgtgaaaaac aaactgtcga aaaacgggaa aatcactgaa actgtgttaa
    55261 cggaatactt tcgacaaaag tttgcggaac gtttctttta cgattggcat tctgttccgt
    55321 ctcgttttca acagtacagg caattgtatg ataacgagtc cggtcattta agccgtgcta
    55381 aggaactgat gaccgacttt tcagcgacaa cttcctggct attacctttc aatcatccta
    55441 acggcgaagc ggtaaaacca catccttttc aacatcttgc tcgacagcat aaaatggtag
    55501 acgttggatt tgcctatttt tatgatcaga aaaatc
//
LOCUS       DAOSIB010000003        54562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_693_length_54562_cov_13.218559, whole genome shotgun sequence.
ACCESSION   DAOSIB010000003 DAOSIB010000000
VERSION     DAOSIB010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 54562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54562
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_693_length_54562_cov_13.218559"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..3241
                     /locus_tag="PLD44_00670"
     CDS             32..3241
                     /locus_tag="PLD44_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOF97883.1"
                     /translation="MAIIPLLKAQPTLKPNDYDITKDRLLYTVGYAHLDTEWNWDYPT
                     TINEYILYTMVENFALFEKYPDYVFNFTGSRRYKMMKEYYPQLYNQVKNYVEQGRWKV
                     SGSSVDEGEVNISSSESLVRQVLYGNEFFKNEFGKESVDFMLPDCFGFLWNLPSVLNH
                     CGLIGFSTQKLTWRSAAGVPFNVGVWKGPDDKGIVAALNATRYTSKVEERLDKNPQWV
                     ERLNQDKEKTGYAFDYRYYGVGDKGGAPRINDVKRAVNSLHNDDSNFKVLLTSSDQMY
                     KDITPEIKAKLPVYSGDLLLIEHSSGSLTSQAFMKRMNRKNELLASSAELASVAADWM
                     GTAAYPFGKLNNGWDLVLGSQFHDILPGTSIPKAYEYAWNDEFIAANCFAEALKNSLS
                     SIAGKMNTQTEGKAIVVYNPVSRARQDVVTAQLEYKSLPQTVGVFDKNGKEVPSQIIE
                     RNGNGIKILFLADMPSASIKVYDVREISNAVKQTALSISENSLENEYYKVKVAPNGDI
                     LSIYDKKNTKELLAKPARLEFLAETPDEWPAWNMDWKDRQKPAIDFMDKNAKISVVEQ
                     GPVRVTLKVERAGQNSTITQFYSLAAGEAGKRLEVSNKIDWQSRAVSLKATFPLSVSN
                     KNATYNLGVGTISRGNNEEKKFEVPSKQWFDLTDQSGKYGVTILEDCKYGSDKPDDNT
                     LRLTLLYTPGIHLPQWQRTMYQSTQDWGIHDVKYAVYGHQGDWRKGESAWQAQFFNQP
                     LMAFESTKHDGTLGNSFSMISISRPEVGLMAFKKMESSEGYVIRVNELSGKDIKNAKI
                     TLPGEILQAYEVNGQEKRIGDVNFTKNSIQFDLSHYTIKSFAVKLKKANEAVLPQQPV
                     TIPYNADIMSYDENRDDGVMFDGQSFPAELLPEYVESEGIRFKIGNKQDEQNNAVACN
                     AQTIALPAGNFTKLYLLAAAANDVKADFYVDGNPINLSIQKWTGFVGQFYNRILSLDQ
                     NKVLKMNEPFTKTDNIAWFASHCHNKYPMKNEAYQYSYLYKYEIPIVAGAKNLTLPNN
                     RNIKIVAITVAAPKADNLKTLQAMYDDFENNPTFHLREK"
     gene            3712..4659
                     /gene="cysK"
                     /locus_tag="PLD44_00675"
     CDS             3712..4659
                     /gene="cysK"
                     /locus_tag="PLD44_00675"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOF97884.1"
                     /translation="MSKIAKNLTELIGNTPLLQLTHFVSNKGLQANIIAKLESFNPAG
                     SVKDRIALAMIEDAEEKGLLKQGSEIIEPTSGNTGIGLAMIATVKGYKLTLTMPETMS
                     VERRNLLKALGANIVLTPGAEGMKGAIAKAHELHDQNPSSFMPQQFENPANPLKHFHT
                     TGEEIWRDTEGKVDIFVAGVGTGGTISGVGAALKKHNPAIKIVAVEPASSPVLSGGKP
                     GPHQIQGIGAGFVPKNFDPTVVDEIIQVGNDDAIRTSRQLAQQEGLLVGISSGAAAFA
                     ASLLSQRPENKGKNIVVLLPDTGERYLSTALYAFDEYPL"
     gene            4693..5994
                     /locus_tag="PLD44_00680"
     CDS             4693..5994
                     /locus_tag="PLD44_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOF97885.1"
                     /translation="MNINNLKFETLQVHAGQEVDKTTKSRAVPIYQTSSYVFDDAADG
                     ADLFALRKFGNIYTRLQNPTTDVFEKRMAALEGGISALATSSGQAAEFLALNNIVQAG
                     DNFVATSHLYGGTYNQFKNQFKRLGVDVKFTANDEPETFEKLIDDKTKAIYIETIGNP
                     DLNIPDFEAIAAVADKYDIPLIVDNTFGAGGYLFRPIEHGASIVVESATKWIGGHGTS
                     LGGVIVDSGKFNWGNGKFPAFTEPSESYHGLVFWDAFGADSPFGNIAFNIRARVEGLR
                     DWGCVISPFNSFLLLQGLETLSLRMERHVQNALELARWLQTLSAVEYVNYPGLENNPY
                     HEMAKKYLKKGFGGVLAFKLKGDASMADKFINNLKLISHLANVGDAKSLIIHPATTTH
                     EQLSPEDKIAAGVFPGLLRFSVGIENIDDIKADIEQALSNI"
     gene            complement(6209..7546)
                     /gene="sufD"
                     /locus_tag="PLD44_00685"
     CDS             complement(6209..7546)
                     /gene="sufD"
                     /locus_tag="PLD44_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOF97886.1"
                     /translation="MEQQYIDFYKEYHTLIKQHSAALMNNFRDVAFEKFERLGFPTSK
                     LENYKYTDMSAPLSIEYGLNINRLQFQINPYDVFKCDVPAIHSYLFFVLNDTFYPVNS
                     TAKQHLPEGVIIESLRTAAENYPQLVEKYLGKLSNEKNDGWIAFNETFAQDGFFMYVP
                     KNVKLSKPVQLINIMRSDVDFMANSHNLIILEEGAKAQLLVCDHTMDNVHFFSNRLTE
                     VFAGKNAVYEHYKLENTNILNTNVSNLLIHQEDSSNVLTNVITLHNGMTRNGIEMIID
                     GENCESLLCGMVIGDKNQDVDNFTEIVHKKPNSQSRELFKYVLDDSSKGAFAGKIQVA
                     KNAQKTSSFQTNKNILLSKTAKMRTKPQLEIYADDVKCSHGATIGQLDEAAKFYMQSR
                     GISESEARLLLMFAFTADVMDNIRINALRDRMKLLVEKRLRGELSRCEGCMIC"
     gene            complement(7581..8345)
                     /gene="sufC"
                     /locus_tag="PLD44_00690"
     CDS             complement(7581..8345)
                     /gene="sufC"
                     /locus_tag="PLD44_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOF97887.1"
                     /translation="MLEIKDLHASINGKEILKGVNLTIRAGEIHAIMGPNGAGKSTLA
                     WVLCGNPAVEVTHGEIIFNGKNLLEMSPEDRSREGIFLSFQYPVEIPGVSMVNFMRTA
                     VNEQRKYKNLEPLSAADFLRLMREKKELVEMDNKLTSRSVNEGFSGGEKKRNEIFQMA
                     MLEPKLAILDETDSGLDIDALRIVANGVNKLKRPDNATLVITHYQRLLDYIIPDFVHV
                     FYDGRIVKTGDKNLALELEEKGYDWIKKEVETPVEQ"
     gene            complement(8401..9837)
                     /gene="sufB"
                     /locus_tag="PLD44_00695"
     CDS             complement(8401..9837)
                     /gene="sufB"
                     /locus_tag="PLD44_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOF97888.1"
                     /translation="MSEDIINQVTQGEYKYGFTTDIDTEVFPMGLNEEIIRLISVKKN
                     EPEWLLDFRIKSYRHWLTMKMPTWAHLEIPPIDFQKISYFAAPRKKSPKSMDEVDPEI
                     LKTFDKLGIPLEEQKTLTGMAVDAVMDSISVKTTFKENLAELGIIFCSFSEAVQHHPD
                     LVQKYLGSVVPYSDNFFATLNSAVFSDGSFVYIPKGVRCPMELSTYFRINASNTGQFE
                     RTLIVADEDSYVSYLEGCTAPMRDENQLHAAIVEIVALKNAEVKYSTVQNWYPGDKEG
                     KGGIYNFVTKRGICKGENSKISWTQVETGSAITWKYPSCILQGDNSSAEFYSVAVTNH
                     FQQADTGTKMIHLGKNTRSHILSKGISAGFGQNSYRGLVRVSKNADNARNFSQCDSLL
                     LGDKCGAHTFPYMDVNNDTAIVEHEATTSKISEDQIFYCNQRGISTEDAVGLIVNGYA
                     REVINKLPMEFAVEAQKLLQITLEGSVG"
     gene            complement(10119..11126)
                     /locus_tag="PLD44_00700"
     CDS             complement(10119..11126)
                     /locus_tag="PLD44_00700"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOF97889.1"
                     /translation="MRVAIVGASGAVGQEFLRVLAERNFPLTSLSLFGSSRSAGSVYD
                     FKGEKLTVKELKHNDDFKDIDLAFTSAGAGISKEFAEDITKYGALMIDNSSAFRMEND
                     VPLVVPEVNASDAKNRPRGIIANPNCTTIQMVVALKAIENVSHIKRVHVATYQAASGA
                     GAAAMAELELQYRQVLAGETPTVNKFPYQLAFNVIPQIDVFTENGYTKEEMKMFNETR
                     KIMHSDVVVSATCVRVPSLRAHSESIWAETERPLSLEEVKAAFENAEGVVVMDNPAEK
                     LYPMPLFLSGKDPVYVGRIRKDLANPNGITFWCVSDQIKKGAALNAVQIAEYLIKEKE
                     F"
     gene            11292..13073
                     /locus_tag="PLD44_00705"
     CDS             11292..13073
                     /locus_tag="PLD44_00705"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOF97890.1"
                     /translation="MKKTCLIFMYLLPVFSFGQVLDDFSDGNFTENPSWSGTTGNFKI
                     NEYFQLQSNAPTTSVSYLFTPSEVFYDAEWQAWFKINYSTSSSNYSAFYIISSSPDNL
                     GNAYFVKIGDTQDDVSLWRQQGTSKIKIIDGTDKRTDGTQVEIRVKVTRDSLGYFSLY
                     SKLPSESDFVLEGTVRDTTIESSSYIGLLYSNTTTTTSAYFFDDIAANGKKAVDKKPP
                     VWEKLAIIEPDTLQLQFSEPVDLSNSIFILNENEITPTKIDILSTKPFLMNVALNENF
                     DKGKIYTLELLQVTDLAGNTLLDNIKKTGIIEPLAQGDLLLNEVMFNAPPTATEYIEI
                     YNASAKTLDVSGIVITTRKSDGSLNTLRHIPDGKLLAAGGYLAVCQHADSVMNYFGLP
                     NDGSIVSISWTALNNQQSTIVLANSKADTVYDELTYNEKWHHLMIKNRQGVALERINP
                     ALPTQSAESWHSAASEVKYGTPGYKNSQYREIENTFSNKQFVWVEPEAFSPDNDGTDD
                     VCFIKYRTPTEGFVANAQIINATGTKVFQLVSNALLSSEGFFSWDGRTDSGKIANAGI
                     YVLYFEMIQPKTGQKKQMKIPIVVSAR"
     gene            13171..13893
                     /locus_tag="PLD44_00710"
     CDS             13171..13893
                     /locus_tag="PLD44_00710"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOF97891.1"
                     /translation="MRKIFVLSLLAIFLITSAEAKKPKKIQEEPPVQKLTNTIDSMSY
                     ALGLSIGSDFIKNLKTIPGGQFNIDWLIQGFTGGMKKDSTVMTTAFANNYFREYITKA
                     QSLESAKKKEVADAFMEKNKTRESVITTESGLQYEILACANGPKPSINDTVEVNYQGF
                     LPDSTKFDSTFDRGEPAVFALNQVIPGWSEGLQLMTVGSKYKFYIPYELAYGEQGAGG
                     VIPPYSPLIFEVELLQINPAKK"
     gene            13934..14461
                     /locus_tag="PLD44_00715"
     CDS             13934..14461
                     /locus_tag="PLD44_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HOF97892.1"
                     /translation="MKKQIGFMLATVLAVFLLASCSKKYEAKKVQLKTQNDSLNYTLG
                     LANGEGIKNYYLQNDSSDAPIKALINALDKAYSSKEVDDQMYQLGYQIGTSLKYQKEN
                     GLMGDSTLVFNTKLFKLGLVNGLHGFDGGMTPQQAQEYLQKTMTELQAKKSAQQAPAN
                     QPQPAPESQKTDSVQ"
     gene            14511..15374
                     /locus_tag="PLD44_00720"
     CDS             14511..15374
                     /locus_tag="PLD44_00720"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOF97893.1"
                     /translation="MKKLNLFLLVAISATFTFSSCNSDKPGKPTLKSQIDSLNYAFGV
                     ANGDGIKNYYLQGDSTGKALNSLLKGINDGLKSKVDKENMELASIGSQIGYSLKEQSK
                     SGLMGDSSLSVNINLVKQGLINGMKGSNIQMTPQEASEYLQSTMQSLQEKKIEKQYSA
                     NKEAGEKFLLENAKKSGVITTESGLQYEIIKKGKGPIPTDSSKVKVHYHGTLIDGTVF
                     DSSVERNQPAEFYVNQVIKGWTEALKLMPVGSKFKIYVPYQLAYGSANQGAIKPFSML
                     IFEVELLAIEK"
     gene            complement(15814..16551)
                     /locus_tag="PLD44_00725"
     CDS             complement(15814..16551)
                     /locus_tag="PLD44_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOF97894.1"
                     /translation="MLNDTSDDLMENHPADDIYNNIWTSERLNPYHFPVDSLPDSVRI
                     DCSNFILPIYGKVTSKFGRRRYRYHYGIDLKLNVGDSVRCAFEGKVRIIDYEHGGYGH
                     YVVVRHNNGLETVYAHLSKVLVELNQIIGAGEVIALGGNTGRSTGPHLHFEMRYLGNA
                     IDPDNLVDFEKGSIRQFSYLITKKNTFKYQKDLKSLQMARYHVVHKGDTLSLIASRYG
                     TTVKQLCNLNQISSKKLLKPGQKIRVR"
     gene            complement(16724..17185)
                     /gene="ndk"
                     /locus_tag="PLD44_00730"
     CDS             complement(16724..17185)
                     /gene="ndk"
                     /locus_tag="PLD44_00730"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOF97895.1"
                     /translation="MEKTLVIIKPGAIQRELIGEIITRFERKGLHLCGIKMYQFTDKV
                     VSEHYAHLADRPFFKRIKDSMMVTPVIVCCWEGLDAVRIVRSMSGVTNGREAAPGTIR
                     GDFSMSVQENIVHASDTVENAKVEIDRFFKPEEIFEFKPNNFPFVYANDEV"
     gene            complement(17286..19382)
                     /gene="recG"
                     /locus_tag="PLD44_00735"
     CDS             complement(17286..19382)
                     /gene="recG"
                     /locus_tag="PLD44_00735"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOF97896.1"
                     /translation="MDFFTQDIQFIPGVGPKRAELFKKELNIRTAEDLLRHYPYKYVD
                     RSRFYMISEISENMPFIQLKGKILKFEKVGEGCSQRLIGVFTDGRQYIELVWFKGLKF
                     VAERYKTNIEYVVFGKPTLFNGKYNIVHPDIEILSQLPPVEQMGLQPFYNTSEKMKTH
                     FLNSKAIQKIIYSLLPTLQNGIPETLPAYILNRYGLLNLKDALVNIHFPKNSHLLNRA
                     RQRLKFEELFYIQLDIVKQTAWRNQRFKGFVFKHVGYYFNTFYRDYLPFELTNAQKKV
                     IREIRSDVASGKQMNRLLQGDVGSGKTLVALFAMLMAVDNGFQACMMAPTEILAHQHF
                     ESLKQFLGNIGIQIALLTGSTRPKERAAIHEKLLSGELNILLGTHALIEETVQFKNLG
                     LVVIDEQHRFGVAQRARLWSKNVQPPHILVMTATPIPRTLAMTIYGDLTVSVIDELPP
                     GRKPVQTFHFYDNKRQALYKFMVKQIEAGRQIYVVYPLIQESETMDLKNLEEGYQHLS
                     EIFPQYQISMVHGKMKPAEKEAEMQKFVAGKTQIMVATTVIEVGVNVPNASVMIIENA
                     ERFGLAQLHQLRGRVGRGADQSYCILLTGYKLSNLTRKRIETMVNTNDGFQIAETDLQ
                     LRGPGDLDGTQQSGLPFELKIANLAQDGKMLEMARKVAMDIIEEDPQLQKKENLILSE
                     QLKKMKTSSLNWSVIS"
     gene            complement(19415..20149)
                     /locus_tag="PLD44_00740"
     CDS             complement(19415..20149)
                     /locus_tag="PLD44_00740"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOF97897.1"
                     /translation="MELTKIVLIVAGGKGERMQSDIPKQFIEIAGKPILMHTIEAFAR
                     YDFDLPVFVVLPVTQIEFWKNLCSKYSFKFSHQVVIGGETRFHSVKNGLEAVAAFLAS
                     LYPDKSEEENNSALVAVHDGVRPLVSVETIRRCFDEAEKFGSAIPSMDLVDSLRTIHT
                     KYSQSLDRSAYKLVQTPQVFDFNILKKAYEQEFSPLFTDDASVVEAIGVNVHLTEGNR
                     ENIKLTTAFDLELASFLLAKRENSSY"
     gene            20349..20780
                     /locus_tag="PLD44_00745"
     CDS             20349..20780
                     /locus_tag="PLD44_00745"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOF97898.1"
                     /translation="MSGGLQAQDESTSVRQNIFEVLQASDGAGKVIIHQDKRIENLIN
                     QKQTAKTGREATLGNGYRVQVFSSNEQRTGKVEAFKIEKEIREAFPNTGVYVNYNSPF
                     WKVRVGDFSSVDEAQAFRNKLLEVFPDLRSQTYVVRDQIVK"
     gene            20804..21397
                     /gene="folE"
                     /locus_tag="PLD44_00750"
     CDS             20804..21397
                     /gene="folE"
                     /locus_tag="PLD44_00750"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOF97899.1"
                     /translation="MDFDITSSRPFDEKKTGEIAQHYQEIIRLLGENPDRQGLLQTPQ
                     RVARAMQFFLQGYEQDAEEIIRTATFTENYRQMVIVKDIDFYSLCEHHLLPFFGKAHV
                     AYIPNKKITGLSKIARVVEIYARRLQVQERMTTQIKQCIQNTLDPMGVMVIIEAQHLC
                     MQMRGIQKQNSITTTSDFTGVFQEARTREEFMNLINR"
     gene            complement(21698..24247)
                     /locus_tag="PLD44_00755"
     CDS             complement(21698..24247)
                     /locus_tag="PLD44_00755"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOF97900.1"
                     /translation="MKPKTYTAEEVKKASFEYFQGDELAATVWSTKYALKDSYGNFYE
                     LTPDDMHKRLAREIARIEKQYPNPLTEDELFELFRHFKYIVPQGSPMTGIGNDFQIAS
                     LSNCFVIGFDHDADSYGGIIKIDEEQVQLMKRRGGVGHDLSHIRPKGSPVKNSALTST
                     GIVPFMERYSNSTREVAQDGRRGALMLTVSIKHPDSESFIDAKLEAGKITGANISVKI
                     HDDFMQAALAQETYTQQFPIDDDHPWFKKEIDAHSLWKKIIHNAWQSAEPGVLFWDTI
                     TRESVPDCYTDLGFKTISTNPCGEIPLCGYDSCRLLAINLYSYVNHPFTPEASFDFDL
                     FKKHVAVAQRIMDDIIDLEMEKIDKIMAKINADPEDEEIKRTELKLWEKIKNKTSMGR
                     RTGVGTTAEGDMLAALGLRYGTNSAIDFSEQVHKILALAAYRSSVTLAKERGAFSIYD
                     AKREEKNPYILRLREADPELYDEMVKHGRRNIACLTIAPTGTTSIMTQTTSGIEPVFM
                     PVYTRRRKVNPNDKNVRVDFVDENGDSWEEYVVFHHKFVTWMEANHYSTTQKYTKAEL
                     DELVEKSPYYKATANDIDWLKKVQMQGRIQKWVDHSISVTINLPADTTEELVGQLYEE
                     AWRCGCKGVTVYREGSRAGVLLSVEEKKSEKSGERKICFPQSEEHLIRPKELDCDVVR
                     FQNAKDKWIAFVGLKDGRPYEIFTGLADDEEGIALPKTVTQGKIIKTVDENGNKRYDF
                     QFTNKRGYKTTIEGLSHKFDKEFWNYAKLISGVLRYGMPIDQVIKLVSGLQLDSESIN
                     TWKVGVERALKKYIPDGTEANGKNCPSCGQKTLIYQEGCLTCKNCGYSHCG"
     gene            complement(24547..25638)
                     /locus_tag="PLD44_00760"
     CDS             complement(24547..25638)
                     /locus_tag="PLD44_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOF97901.1"
                     /translation="MYIESINIVNYKNIRQAELSFSPKINCFIGNNGMGKTNLLDAVY
                     FLSFCKSHSNFIDSQNIFHEADFAVLQAKYRMGEATEEIYCGMKRNQKKQFKRNKKDY
                     ERLSDHIGLLPLVLISPEDSVLISEGSDERRKFVDGVISQYDKKYLHNLLLYNNALKQ
                     RNALLKVETPVDESLFEILEEQMADYGSYIFEQRTRFIDQLIPVFQQYYRYISSHENE
                     EIDITYLSHFQQNDIKKQMKSTRERDKMLGFSTRGIHKDELEMLLNGYPIKRVGSQGQ
                     NKTFLVSLKLAQFKFLKQTHHQPPILLLDDIFDKLDADRVRKIVELVSQETFGQIFIT
                     DTNREHLDQILYQLDQEAKIFKVENGIIL"
     gene            complement(26182..28479)
                     /locus_tag="PLD44_00765"
     CDS             complement(26182..28479)
                     /locus_tag="PLD44_00765"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOF97902.1"
                     /translation="MTKLTKEDALLYHSQGKPGKIEVVPTKPHSSQRDLSLAYSPGVA
                     EPCLEIEKDPATVYDYTNKGNLVAVISNGTAVLGLGDIGAMAGKPVMEGKALLFKIFA
                     GIDVFDIEVNEKDPEKFIQAVKAISPTFGGINLEDIKAPECFMIEERLKAELDIPIMH
                     DDQHGTAIISGAGLINALEIAGKRMEDVKIVVNGAGAAAVSCTKLYIRLGAKPENIVM
                     LDSKGVINKQRTDLNETKKAFATDREINTLEEAVKGADVFLGLSVAGVLSIEMVRSMN
                     ENPIVFALANPNPEISYEDAISAREDIIFATGRSDHPNQINNVLGFPYIFRGALDVRA
                     TCINEEMKLAAVYALAELAKKPVPDVVNAAYNLKRLSFGRDYIIPKPLDPRLLTIVAP
                     AVARAAMESGVARKHITDWAGYNAKLRETMGYDDKMLRSFYETARQNPKRVVFSEANH
                     LNMLKAAEMAYNEGVCFPVLLGNEEKINSIAREYNIDLSGIEIINLRHDREEARRIAY
                     AEKLAKKLGREGMTYDEAYEKMYERNYFGMMMVETGEADALITGVYTKYADAIQAAKD
                     VIGIRPGSKHIAAMHIMNTKKGTFFMADTLFNRHPSTETLIDIAKLTYRTVKFFAHEP
                     VMAMLSYSNFGSDKQGSPASVHEVVETLHREVPEMIVDGEMQVNFALNKELRDRKYPF
                     TRLAGKDVNTLIFPNLSSANSAYKLLLEMGISEAVGPIQMGLNKPVHILDVESSVRDI
                     VNMTAIAVLDAIVEEKITTENLERI"
     gene            28906..30855
                     /locus_tag="PLD44_00770"
     CDS             28906..30855
                     /locus_tag="PLD44_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOF97903.1"
                     /translation="MKEIFKFLNNTDTFSHLASLKYLPRWVVLFIDITISAISFLAAY
                     LVASSFIKHLPDQLLLPLFPRLILLLFTQSVFFWLFHTYSGILRYSTMTDAAKLFLAI
                     FGNCIFLSALNFVCSLYFPAPLFYYASILIYGVISFILLFGLRLTVRNIFNYFVETSA
                     QIIPVMIYGIDEAGLGIARMILSSVNSKKQYKLVGFIDGNENIVNKMVMGLRVYKHDD
                     HTIKTKVANKVNTVIVSPIKMKEINPDVDLDIFIQHHISLLTSQPMDIWEGEMPSVRQ
                     IKPLKIEDLLEREPIQISFDNIAAELKSKTVMVTGAAGSIGSEIVRQLCNFTPKLIVL
                     VDNGETAIHNLKIELQAFSTSQHFNFVLGDVRNKERMEYIMDLYRPDFVFHAAAYKHV
                     PMMEDFPCESVQVNVLGTKIMADLALKYRVSRFVMVSTDKAVNPTNVMGASKRIAEIY
                     VQSLARKMQKTGTETTKFITTRFGNVLGSNGSVVPFFQKQIEKGGPVTVTHPDIIRYF
                     MTVPEASSLVLEAGVMGEGGEIYVFDMGNPVNISHLAKKMIQMAGLIPGVDIQIAYTG
                     LRPGEKLYEELLSHKEKVRSTQHPKIMVASVAEYDFDKISVDIDNLIKYSYLCKDFLV
                     VSMMKQIVPEFKSNNSQYELLDISN"
     gene            30992..32071
                     /locus_tag="PLD44_00775"
     CDS             30992..32071
                     /locus_tag="PLD44_00775"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOF97904.1"
                     /translation="MNYLYNFIQQNPYLLILFSFLLGKAFMPSVVRIAKKHNFVVKPN
                     KRTTHKGLIPYTGGFDIFISFIFTILIFCFPLMEKLQYIPIGIFIILMTGFIDDLLNI
                     KPTSKLLGEFVAGVFLIVFSDIRITNFFGVFGIHELPLSISYIFSFLVFLLIINSINL
                     IDGVDGLASGLGIFYNLFFGIYFHLTGNLSLAMIAYTLTGSLAVFFIYNVFSKKNKIF
                     MGDTGSLFLGFMLTFYLFEFCEMNAYQSVPQSMYMAAAPAVAICVLIVPLYDTLRVVI
                     TRLKKGVSPFKADKNHIHHLLLKIGFSHRQVTFVLLAITAFFTAFALLARQWSNLVLA
                     FIAFLLATGMIFWLWRVINRKMQRK"
     gene            32123..34072
                     /locus_tag="PLD44_00780"
     CDS             32123..34072
                     /locus_tag="PLD44_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOF97905.1"
                     /translation="MISVEQLCVEFGGTTLFDNITFLIHPKEKIALVGKNGAGKTTLL
                     RILAGKQQPTRGNISYQKDITIGYLPQHMIHSEGTTLWQETEKAFGNIVQLRQQLEDI
                     QQQLVERTDYESSAYHSLLDKLSQLTEYLQISGNGNYQAEMEKTLLGLGFLRSDFYRQ
                     CSEFSGGWRMRIELAKILLSHPDVLLLDEPTNHLDIESIQWLENFLFTSGSSLLLVSH
                     DKAFIDAVTTRTLEISLGKLYDYDVNYSKYIQLRKERHEQQLRAYENQQKLIQDTEDF
                     IERFRYKATKAVQVQSRIKQLEKLQRIEVDLEDNANMHLKFPPAIHSGKIPLEIEHLS
                     KAYREHLVLDDVNLMMHRGEKVAFVGKNGEGKSTLVKCIMNEIEYSGTLKLGYNVKIG
                     YFAQNQASLLDENLTVFETIDRVAVGEIRTKIRDILGAFMFGGENSEKKVKVLSGGER
                     SRLAMIKLLLEPVNFLILDEPTNHLDIPSKEVLKEAIKAFDGTAIIVSHDREFLDGLV
                     TKVYEFGNKKVKEHLGGIYDFLQAKKMESLHELERNVSSEVKNEGEAKPESENKLNYE
                     SRKELNRKIRKLEKDVEATEGLITDLDNEIQQMNTDLQIPEKASDPEFVSTLQKKRAE
                     LERKMYEWEILSEELEKIRKEWQGQ"
     gene            34140..34844
                     /locus_tag="PLD44_00785"
     CDS             34140..34844
                     /locus_tag="PLD44_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOF97906.1"
                     /translation="MKTLYLIPTPLGNTSFDRIFPPVNLEIIRNLRYFIVEDIRTARR
                     FLKQVDVEIDIDKLFFYTLNEHTSPTEVDGFLQPLREGEDMGLLSEAGCPAVADPGAD
                     MVAIAQKNAFRVVPLVGPSSILLALMASGFNGQNFAFVGYLPVSPAERIKAIRKLESR
                     AYSENQTQIFIETPYRNLKLFEQILQNCRPSTLLCIAANVTLPDESIVTRSVAEWKTI
                     TPDIDKKPCIFLIYKG"
     gene            complement(35075..36307)
                     /locus_tag="PLD44_00790"
     CDS             complement(35075..36307)
                     /locus_tag="PLD44_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOF97907.1"
                     /translation="MNELKEKTNFSIDDFDYVLPEERIAKYPLKKRDASKLLIYRDGT
                     ITENVFSNIASFLPEKALLVYNNTKVIQARIHFRKETGALIEVFCLEPAQPADYALSL
                     SATKECVWKCFVGNQKKWKNGTLSKELDINGTQFEFSAELLERKGNVNFVRFSWNNEN
                     IHFAEILEKAGELPIPPYLHRPTEESDLITYQTVYAKIKGSVAAPTAGLHFTPEVFES
                     LKSRNIETAELTLHVGAGTFQPVKTKQISDHQMHTEVIEIQKKTIEKLLKNIGKIIAV
                     GTTSVRTLESLYHIGLLIEKQQFPDEKHPYFFIPQWMPYQTECVLTPEASLQLILNEM
                     EKRNLSVLHAQTQLIIQPGYTFRLTNGMITNFHQPKSTLLLLVSAFVDGNWRKIYDYA
                     LSHDFRFLSYGDSSLLMK"
     gene            36447..37532
                     /locus_tag="PLD44_00795"
     CDS             36447..37532
                     /locus_tag="PLD44_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HOF97908.1"
                     /translation="MIKVGDKVRFLNSTGGGIVTRIDEREKIVYVSDADGFEIPMLER
                     ECVVIPEVNENTNFPKKDFKSASAAFMNENESPAAKSINLPPKPEIIEEEIFETPEGE
                     NLRVFLAFVPEDIKQLQTTSNDFYLVNDSNYFLFYNLALFENECCKSVANGLIEPNMQ
                     EWMAEIKKEELNAWEKIRVQIIPFKKHKTYRPQPVIDIDLKLNLMKFYKLHSFGENEY
                     FDVPAMMLNPLEEIETHQLHDISAEEIKQAISQKEKPERRPRISKPAKNPEIVEVDLH
                     INQLVDTTVGMNNADMLELQLEKFESVLNDYKNRKGQKIVFIHGKGEGVLRKEIEKLL
                     RTKYKSYYFQDASFQQYGFGATMVTIR"
     gene            37808..38698
                     /locus_tag="PLD44_00800"
     CDS             37808..38698
                     /locus_tag="PLD44_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97909.1"
                     /translation="MIDKFFIKRYFLFVFSLILSVSFFSCGEKEENEPDITNSIYVTQ
                     VFEYVYAPGQHAQLAKPADIEHFIGEPSDEKGFLYLGGFGGYVIAGFDHDIQNKDGYD
                     LEIFALPGASPEPAIVYVMPDDNGDGKPNDTWYELKGNQFENEKRNYWVRYYKPATSN
                     DNIQWKDSEGENGELISGFGAPNTSSWWWATCMEDSITFEGSRLPDAYNNKSSESAQS
                     WIVPTDRFIWGYAENNFGTDYDTHHHSNKLDISNAVDSSGKAVYLEKIRFIKIQTAVF
                     QRAGWVNEVSAEIRGAAELK"
     gene            38828..40270
                     /locus_tag="PLD44_00805"
     CDS             38828..40270
                     /locus_tag="PLD44_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOF97910.1"
                     /translation="MENLEPSVVWKFFGEITQIPRPSKKEEKIREYLLDVAQQYRLEA
                     KTDQAGNVLICKPATFGYENHKKIILQSHMDMVCEKDGDVQHNFDSDPIQTFIDGDWI
                     KAEGTTLGADNGIGMAMMLAVLTSDNISHPALECLFTVDEETGLTGASAIEDHFLSGQ
                     ILINLDSEDDGVIFIGCAGGIDTTINFPFIAEKSPNNYFSFSLTVKGLKGGHSGDDID
                     KRHGNAIKILNRFLWNVNKKLDLRIHTFRGGNLHNAIPREAEVVASVPFEDKEKLRVL
                     LNEFIAEIEGELKEDEPALQILISSEEIPDQVLDKKSSDKLLNALYACPHGVKAFSRD
                     MPNLVETSTNLASVRMQENNSFLIITSQRSSVESAKFDIAHQVESVFTLAQAQATHGE
                     GYPGWKPNLNSEILQKAKVCYKNITCEEPKVRAIHAGLECGLFLKKYPYLDMISIGPQ
                     MYGVHSPNERLSISSTRKCWQWLLKILEKS"
     gene            40272..41540
                     /locus_tag="PLD44_00810"
     CDS             40272..41540
                     /locus_tag="PLD44_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOF97911.1"
                     /translation="MTNISGNNSLGESRIQPNKLKIINDPVFGFINIPDDFLYEIIQH
                     PYFQRLNRIKQLGVSFYVYPGAQHTRLQHSLGAMHLMGEAIEELRQKGHVISGEEEQA
                     ARACMLLHDIGHGPFSHALENSLVKGIHHEEISIMLMQQLNKEMNNNLALALEIFSGH
                     YPKKFLHQLVSGQLDMDRMDYLSRDSFFCGVSEGIIGSARIIKMLNVFHGELVVELKG
                     IYSIEKFLVARRLMYWQVYLHKTLVATEKMLTNILKRAKELASVGETLFASPALHYFL
                     YHHLDKQNFVRSTEALDYFVQLDDSDVICAIKVWAHHPDKVLSFLCNAFINRKLFKVE
                     MLQEPLDETKKEELLKKYSRYFDVSQQEAEYFMGEEIVSTDTYDPVDEKINILYKDGT
                     VKDIADASDMLNINVLTKKVYKNYFCYCRI"
     gene            41655..42707
                     /gene="lpxD"
                     /locus_tag="PLD44_00815"
     CDS             41655..42707
                     /gene="lpxD"
                     /locus_tag="PLD44_00815"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOF97912.1"
                     /translation="MEFTAQQIADFLGGQVEGDPSVKVSNFSKIEEGKPGTITFLSNP
                     KYSHYIYDCNASIVLVNKDFKPEKEIPATLIRVNNAYECLAQLFSLVEQSKPKKKGIS
                     PLAFIASTAQIGEDVYIAPFVYVGENVKIGKNTQIHSHSNIEDNSSVGENTILFSGVH
                     VYPDCSIGNNCILHSGVVIGADGFGFAQSEDGTYKKITQIGNVVLEDDIEVGANTTID
                     RATLGSTIVHKGVKIDNLVQIAHNDEIGENTVIVAQTGIAGSTKIGKNCVIGGQVGIV
                     GHLQIADNTQIGAQSGVANSVKTPGQIIIGSPAIPIQNFRRSSIVFKNLSDLQKMVIE
                     LQNKVEELEKTKTSEK"
     gene            42768..44150
                     /locus_tag="PLD44_00820"
     CDS             42768..44150
                     /locus_tag="PLD44_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOF97913.1"
                     /translation="MTRQRTLKESFTLEGKGLHTGLDIVITFLPAPENHGIKLKRIDL
                     EEPVIIEALAENVTHTVRGTVLQKGDVQVSTVEHALAALYAFGIDNCLIEVNAPEFPI
                     LDGSARFYAEKIKEVGIIEQKAEKDFYVVRKKIEYSLPDSNSKITLLPDDVFSVDVHI
                     GFDSAVLANQYASLDSLDKFADEIASARTFVFVHEIEPLLSMNLIKGGDLKNALVIYD
                     KELSQDTMDRLTDKLHQPRIDATKLGYINGELNYPNEPARHKLLDVIGDLSLIGKPLK
                     AKVIVQYPGHKINTNLAKIIRKEIKKQEVSAPVYDDSVPPVLDINAIRKLLPHRWPFL
                     LVDKIIDINERVIVGVKNVSGNETFFMGHFPNEPVMPGVLLVEAMAQTAGILVLNKVE
                     EPEKYSTYFMKIDNVKFRQKVVPGDTVIFRLEMMDEIRHGCTDMKGYAFVGGKIVAEA
                     QFMAQIIKNK"
     gene            44175..44954
                     /gene="lpxA"
                     /locus_tag="PLD44_00825"
     CDS             44175..44954
                     /gene="lpxA"
                     /locus_tag="PLD44_00825"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOF97914.1"
                     /translation="MKQPLAYIHPDAKIAPSVVIEPFVTIDKNVEIGEGTRIGPNVTI
                     LEGARIGKNCTIFPGAVIAAIPQDLKFKGEDTLAIIGDNTTIRECVTVNRGTASKGKT
                     QIGDNCLIMAYCHIAHDCFVGSNVIMSNATQLAGEVFIDDYAVISGGVLVHQFTHIGS
                     HVIIQGGTKVNKDVPPYVKAGREPLSFEGINSVGLRRRGFTNEQIGFIQEIYRYIFLS
                     KMNVSNAIDRIEAELPATKERDDILLFIKNSQRGIIKSFLE"
     gene            45010..46701
                     /locus_tag="PLD44_00830"
     CDS             45010..46701
                     /locus_tag="PLD44_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOF97915.1"
                     /translation="MKINEEETPKKNLNFIEAIVDQDVKEGKNGGRVQTRFPPEPNGY
                     LHIGHAKAICLNFDIAKRYGGICNLRFDDTNPVKEDVEYVDSIMEDIQWLGYQWKNVY
                     YASDYFQKLWDLAVQFIKKGLAYVDEQSAETIAKQKGTPTVPGIESPYRNRPIEENLA
                     LFQKMTNGEIPEGGMVLRAKIDMASPNMHFRDPIMYRIITSHPHHRTGWQWKVYPMYD
                     FAHGQCDYFEGVTHSLCTLEFEVHRPLYDWFIDQYQDTDYRPRQIEFNRLNLTYTVMS
                     KRKLLQLVQEKFVSGWDDPRMPTLCGLRRRGFTPESIHNFIDKIGYTKFEALNDIGLL
                     EHAARESLNKTAMRVNAVLHPVKLIVTNYPEGKVEMMTTQNNPEDPEAGTREVVFSRE
                     LFIERDDFMENPPKNYFRLMPGQEVRLKSAYIVKCTGCKKDENGVVTEVYCEYDPMTK
                     SGMPESNRKVKGTLHWVSAAHCVEAEVRLYDRLFNVENPSADERDFRELLNPDSLQIL
                     TGCKLENSLKDAKPLEHFQFQRIGYFTVDLDSTPDKLIFNRTVSLKDSWSKELNK"
     gene            47388..47963
                     /locus_tag="PLD44_00835"
     CDS             47388..47963
                     /locus_tag="PLD44_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOF97916.1"
                     /translation="MDFSLIQIASAFMVLFAIIDILGSVPIILAIKSKGENVHAGKTS
                     LVALVILVVFLFAGDWILQLFNVDIESFAVAGSFVIFIFSLEMILDVEIFRNKGPEGA
                     SSVVPIAFPLIAGPGSFTTLLSLRAEYAIENIIIALVLNICFVFFVLRFTSKIEKWMG
                     KGGIYILRKFFGIILLAVSVKLFTTNIRFLL"
     gene            complement(48026..49192)
                     /locus_tag="PLD44_00840"
     CDS             complement(48026..49192)
                     /locus_tag="PLD44_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOF97917.1"
                     /translation="MKNQRYDLRGVSASKEDVHNAIRNLDKGIFPKAFCKIIPDILGN
                     NPDYCNIMHADGAGTKSSLAYAYWKETGDLSVWKGIAQDALIMNTDDLLCIGAVDNIL
                     VSSTIGRNKNLIPGEVVSAIINGTDELIQQLGEMGVKLYFTGGETADVGDLVRTIIVD
                     STVTCRMKRADVIDNANIRPGDVIVGLSSSGQASYEKEYNGGMGSNGLTSARHDVFAH
                     YLAEKYPECYDDKIPSELVFSGSYHLTDKIENLGIDVGKLVLSPTRTYAPIVKKMLEK
                     LRPQIHGMVHCTGGGQTKILHFIENLRVIKNNLFPLPPLFQIIQQESQTDWKEMYKVF
                     NMGHRMEIYLPEAYAEEVIDISRSFNVEAKIIGYCEPSDKKELIIESEFGKFEY"
     gene            complement(49282..49500)
                     /locus_tag="PLD44_00845"
     CDS             complement(49282..49500)
                     /locus_tag="PLD44_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97918.1"
                     /translation="MNKPTLKSLLKTLLYGLGVFGVYIVIIVLLRLFSGRVPDEAVIF
                     NFFTKNDLLIGLLLACILTFTHQRNKKI"
     gene            complement(49520..52381)
                     /gene="uvrA"
                     /locus_tag="PLD44_00850"
     CDS             complement(49520..52381)
                     /gene="uvrA"
                     /locus_tag="PLD44_00850"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOF97919.1"
                     /translation="MNNTNTDTSEKIEVIGARVNNLKNIDVSIPRDALTVITGLSGSG
                     KSSLAFDTIYAEGQRRYIETFSAYARNFLGNLQRPDVDKITGLSPVISIEQKTVNKNP
                     RSTVGTTTEIYDFLRLLFARAGEAFSYVTGEKMVKYSEDQIVELILKQYEGKRTYLLA
                     PLVRGRKGHYKELFEQIRKKGFLHVRIDGEMKELVPGLRVDRYKNHNIEVVIDRLVVA
                     EKDRRRLKDSMQKAMQQGNGIVMLLEKETDKVRYFSKKLMCPTSGISYDDPDPHHFSF
                     NSPHGACPTCKGLGFVNQVDMDKIIPDKSVSIYNGGIAPLGKYRNTVIFWQIEAILEK
                     YGCQLKTPVKDIPEEAIDEIMNGSEEPLMIKNHSLGITNYSLNYDGILKYIEMQQDDR
                     ASATEQKWANQYSKSVKCPECNGARLRKESLHFLIHEKNIADLAAMDIVELLNWLGNV
                     ENYLTEKQKAIATEILKEIRTRLQFMVDVGLHYLSLGRSSESLSGGESQRIRLATQIG
                     SQLVNVLYILDEPSIGLHQRDNFRLINSLKQLRDSGNSVIVVEHDKSMMLAADYIVDM
                     GPRAGRLGGEVVFAGKPEEMLKAKTLTSAYLNGEKRIEIPARVRKGNGKFLTIIGAKG
                     NNLKNIDVSFPLGVMICITGVSGSGKSSLITETLQPILSQHFYRSLQDPLPYDRMEGL
                     ENIDKVVEVDQSPIGRTPRSNPATYTNVFSDIRNLFATLPEAKIRGYKPGRFSFNTAG
                     GRCETCSGNGYKTIEMNFLPDVYVVCETCNGKRYNRETLEVRFKGKSIADVLDMTINQ
                     AVEFFENQPSILNKIKTLQDVGLGYIKLGQSSTTLSGGESQRVKLATELSKRDTGKTL
                     YILDEPTTGLHFEDIRALLDVLNRLVDKGNTVIIIEHNMDVIKSADYIIDLGPEGGNA
                     GGKVICTGTPAEITRHPESETGKYLKMELDENFMEDLID"
     gene            complement(52552..53274)
                     /locus_tag="PLD44_00855"
     CDS             complement(52552..53274)
                     /locus_tag="PLD44_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOF97920.1"
                     /translation="MKINNFLFSVYQWIICVPIVIVLTLLTSIFTIILSPIFPNSNIS
                     YLPARWWSKATCGLFFIKVKVKGLEKIERGKSYVFAANHQSMFDIFVIYGWLPVRFKW
                     IMKKELRKVPFVGLASEMAGHIFVDRSNAQAAKLSIEKAEKQLKKGNSIVIFPEGTRS
                     TNGQMRKFKKGAFQIAADLALPIVPVTIKGSFERLSKTSFKVKPGVIEMIIHQPIDVT
                     PYLPDRIPELMKTSWEMIHSVL"
     gene            complement(53453..54346)
                     /locus_tag="PLD44_00860"
     CDS             complement(53453..54346)
                     /locus_tag="PLD44_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOF97921.1"
                     /translation="MSRFKSLSLYASIVAAMLIWAFSFIWTKEALTSFRPMLLITCRL
                     IIASVLMLIVSILSGRFQKIQKQDLKWFLLLALFEPYLYYIGETFGLTMVSSTLASVI
                     ISTIPVFSPLLAFLFLKERIGWFNVAGIILSLTGVFLVIFEPSGNFHVQPLGIVLLFF
                     AVFSAVCYAVVLQKISIHYYTLNIILYQSLFGLMFFIPTTLLADYPYFSTLHFTLSSI
                     EALLMLSIFASVIAYVLFARVVRRIGVARSNVFTNLIPVFTAIFSWLLLNEQLTILKW
                     IGIATVVGGLFVSQARKIKFK"
BASE COUNT        16649 a        10351 c        10728 g        16834 t
ORIGIN      
        1 gaaaaaacaa ttatttacaa ttttgatttg tttggcaata attccattgc taaaggctca
       61 gccaacgtta aaaccaaatg attatgacat aacaaaagac agacttttgt acacggtggg
      121 ttatgctcat ttggataccg agtggaattg ggattatcca acaaccatta atgagtatat
      181 tttatataca atggtcgaaa attttgctct cttcgaaaaa tatcccgatt atgtattcaa
      241 ttttaccgga tcaaggcggt ataaaatgat gaaagagtat tatcctcaat tgtataatca
      301 ggtaaaaaat tatgttgaac aaggacgttg gaaagtatcc ggctcttcgg ttgacgaagg
      361 tgaagtcaat atttcttctt cagaatcact tgttcgacaa gtgctctatg gaaatgaatt
      421 ttttaaaaat gagtttggga aagaaagcgt agatttcatg ttgccggact gtttcggatt
      481 tttatggaat ttgccttccg ttctgaatca ttgcggattg atcgggtttt caactcagaa
      541 actgacttgg cgttctgctg ctggcgttcc attcaacgta ggcgtttgga aaggccctga
      601 cgacaaaggt attgttgctg cattaaatgc gaccagatat acttccaaag ttgaagagcg
      661 tctcgataaa aatcctcaat gggtggaacg tctgaatcag gacaaggaaa aaacgggtta
      721 tgcgttcgat tatcgctatt atggcgttgg cgataaaggt ggagcaccgc gtataaacga
      781 tgtgaaaagg gctgtcaaca gtttgcacaa tgacgacagt aattttaagg ttttgttgac
      841 ttcttccgat caaatgtaca aagatattac gcctgaaatt aaggcaaaac ttccggtata
      901 ttcgggcgat ttacttttga tagagcacag ttcgggttca ttgacttctc aagcttttat
      961 gaaaagaatg aaccgtaaaa acgaactttt ggcttcgtcg gcagaacttg caagtgtcgc
     1021 tgccgattgg atgggaacag cagcttatcc gttcggtaaa ctaaacaatg gttgggattt
     1081 ggtgctggga agccaatttc atgacatttt gccgggaacg tctattccaa aagcctatga
     1141 atatgcatgg aatgatgaat ttatcgctgc caattgtttt gctgaagcgc tcaaaaacag
     1201 cctgagttcc attgccggta aaatgaatac acaaactgaa ggaaaagcca ttgtagttta
     1261 caatccggta agtcgggcaa gacaagatgt tgttaccgct caattggaat acaaatcgct
     1321 accacaaacc gtcggtgttt tcgataaaaa tggaaaggaa gttccttcgc aaattattga
     1381 aagaaatgga aatggtatta aaattttgtt tttggccgac atgccttctg caagcataaa
     1441 agtgtatgat gtacgtgaaa tttcaaatgc agtaaaacaa actgcacttt ctatttccga
     1501 aaattcactt gaaaacgagt actacaaagt gaaagtagct cccaatggcg atattctcag
     1561 tatttatgat aaaaaaaata cgaaagaatt gttggcaaaa ccggctcgtc ttgaattttt
     1621 ggcagaaaca cccgacgaat ggccggcatg gaacatggac tggaaagatc gacaaaaacc
     1681 ggctattgac tttatggata aaaatgcaaa aatatccgtt gtcgaacaag ggccggtaag
     1741 ggtaactttg aaagtggaaa gagcaggaca aaattcgacc atcacgcaat tctacagttt
     1801 ggctgccggt gaagcaggta aacgtttgga agtgagcaac aagattgact ggcaatcacg
     1861 tgctgtcagt ttgaaagcaa cttttcctct atccgtttcc aataaaaatg caacctacaa
     1921 tttaggtgtt ggtaccattt ctcgaggaaa taatgaggag aaaaaatttg aagttccttc
     1981 taaacaatgg ttcgacttga ctgaccaatc aggaaaatat ggcgtaacga ttttggaaga
     2041 ttgcaaatat ggttccgaca aaccggatga caataccttg cgtctgactt tactttacac
     2101 gcccggtatt catttacccc agtggcagag gacaatgtat caaagtactc aggattgggg
     2161 tattcatgat gtgaaatacg ccgtttatgg acaccaaggt gattggagaa aaggagaatc
     2221 agcatggcaa gctcaatttt tcaatcagcc tttaatggct tttgaatcca ccaaacacga
     2281 tggaacattg ggcaacagtt tctcgatgat ttcgatcagt cgtcccgaag taggtttaat
     2341 ggcctttaag aaaatggaat cgagcgaagg atacgttatt cgtgtgaatg aattgtcggg
     2401 aaaagatata aaaaatgcga aaatcacatt gccgggcgaa atactgcaag cttatgaagt
     2461 gaacggacag gaaaaacgaa taggcgatgt aaattttaca aaaaacagca ttcaattcga
     2521 tttaagtcat tatacgataa aaagttttgc cgtcaaactt aagaaagcaa atgaagctgt
     2581 attgccacag caaccggtaa ctattcctta caatgcggat ataatgagtt acgacgaaaa
     2641 tcgcgacgac ggagtcatgt tcgatggtca gtcgtttcct gccgaacttt tgccggaata
     2701 tgtcgaaagc gaaggcattc gtttcaaaat tggtaataaa caggacgaac aaaataatgc
     2761 tgttgcttgc aacgctcaaa ccattgcttt gcctgccgga aattttacca aactttatct
     2821 tttggctgct gctgcaaatg atgtgaaagc cgatttttat gtggatggta acccaatcaa
     2881 tttgtcgatt caaaaatgga caggttttgt cggtcaattc tacaatcgta ttctgagttt
     2941 ggatcagaat aaagtgctga aaatgaatga gccttttaca aaaacggata atatcgcatg
     3001 gttcgcttcg cattgccaca acaaatatcc gatgaaaaac gaagcttatc agtacagcta
     3061 tttgtataaa tacgaaattc caattgttgc cggagccaag aatttgactt taccaaacaa
     3121 cagaaatata aaaattgttg cgattacggt ggctgctcca aaagctgaca atttaaaaac
     3181 tcttcaggca atgtatgacg atttcgagaa taatccgaca tttcatttga gagaaaaata
     3241 atttttaaag gtgattgcta ttgataattt cgataaatag cacaaaaacc gaattgataa
     3301 atttctcttt aaaacttgtc aaaagattta cagagaaatt tcatcagaat ataatttatt
     3361 ttttaaaggt ctgatggaac ccacatgtct gaatattata ttcattcgtg tttgtgtatg
     3421 caatacatat gggtttcata ataagcaaaa tggaaattct tttcgtttgt ttctcaaatt
     3481 aattatattc ttgatttaca aattattata taaatttgaa tcctaatttt ctccattatt
     3541 gttatttgaa attttgacta taccatcatc gtggtagatt aaattaaccc attcttgtga
     3601 ttgacttgtt tttagttgtt ttgtaccttt gccaaaagaa aaacaaaatc tttagtcaga
     3661 tatttttttc ggtaaaaaag attttgtaaa ttaattaaaa atcaaaagtt tatgtcgaaa
     3721 attgctaaaa atcttactga acttattggc aatacacctt tgttgcaatt aacacatttt
     3781 gtttcaaata aaggattgca agccaatatt attgccaagc tggagtcatt taatccggca
     3841 ggaagcgtaa aggacagaat tgccttagcc atgattgaag acgcagagga aaaaggactt
     3901 ttaaaacaag gttctgaaat tatcgaacct accagcggaa atacaggtat tggtttggct
     3961 atgattgcta cggtgaaagg ttacaaactt acccttacca tgcccgaaac catgagtgtt
     4021 gaacgaagaa atttactgaa ggctttgggt gccaatattg ttttaacgcc cggagctgaa
     4081 ggaatgaaag gtgctattgc caaagcgcat gaattacatg atcagaatcc atcttcgttt
     4141 atgccgcagc agtttgaaaa tcctgccaat ccattgaaac attttcatac cacaggcgag
     4201 gaaatttggc gtgatacgga agggaaagtc gatatttttg ttgccggtgt gggaaccgga
     4261 ggaacgataa gtggagtagg agctgcgttg aaaaaacata atcctgcaat aaaaattgtt
     4321 gcggtcgaac ctgcaagttc tcccgtactt tcgggaggga aacccggacc ccatcaaatt
     4381 cagggaatag gagccgggtt cgtacccaaa aattttgatc cgacggtggt agatgaaatt
     4441 attcaggttg gcaatgatga cgctattcgt acttcacgcc aattggctca acaggaaggc
     4501 ttgctggtgg gaatttcgtc gggtgcggca gcttttgccg cttctttgtt gtctcaacga
     4561 cccgaaaaca aaggaaaaaa cattgtcgtt ctgttgcccg atacgggcga aagatacctt
     4621 tccactgcac tttatgcttt tgacgaatat cctttataaa tttcaagaat cgaaatctaa
     4681 ataattagca atatgaatat caacaattta aaatttgaaa ccttgcaggt tcatgccggt
     4741 caggaagtgg acaaaactac caaatcgcga gcagtaccca tttatcagac ttcttcctac
     4801 gttttcgacg acgcagccga tggagccgac ctttttgctt tgagaaaatt tggaaatatt
     4861 tatacccgtc tgcaaaatcc tactaccgac gtgtttgaaa aacgtatggc tgccctcgag
     4921 ggaggcatca gcgcattggc aacttcatcg ggacaggctg cagaatttct tgctctaaac
     4981 aatattgtac aggctggaga taattttgta gcaacttctc atctttacgg cggaacttac
     5041 aatcagttta aaaatcaatt taaacgtctg ggtgtggacg ttaagtttac ggcaaatgac
     5101 gaaccggaaa cttttgaaaa attgattgac gacaaaacaa aagccattta tatcgaaacc
     5161 attggcaatc ccgatttgaa tatccccgat tttgaagcaa ttgctgctgt agccgataaa
     5221 tatgatattc cgctgatagt ggacaatact tttggcgcag gcggctatct tttccgtccc
     5281 atagaacatg gagcttccat tgtggtagaa agcgctacca aatggattgg aggacatggt
     5341 acttcgctgg gaggtgtaat tgtggacagt ggtaagttta attggggaaa tggcaaattc
     5401 ccggctttta ccgaaccttc tgaaagttat catggattgg ttttttggga tgctttcgga
     5461 gcagatagcc catttgggaa tattgctttc aatataagag cacgtgtaga aggtcttcgc
     5521 gattggggat gcgttatcag tccttttaat tcgtttttgt tgctgcaggg tttggagact
     5581 ttgtctttga gaatggaacg acatgtccaa aatgcgttgg aacttgctcg ctggttacaa
     5641 acattgtcgg ctgtggaata tgttaattat cccggtctgg aaaataatcc ttatcacgaa
     5701 atggcaaaaa aatacctgaa aaaaggtttt ggcggcgtat tggctttcaa attgaaaggc
     5761 gatgcttcaa tggcagacaa attcatcaac aacttaaagc tgataagcca tttggccaat
     5821 gtgggcgatg caaaatctct tattattcat ccggctacca caactcacga gcaactttcg
     5881 cccgaagaca agattgcagc gggagttttt ccgggattac tgcgtttttc agtgggaatc
     5941 gaaaatattg acgatatcaa agccgatatt gagcaagccc tttccaacat ttgattttta
     6001 tttcttaatt ccttatggct gttggaacaa aaccggcagc cttttttaat aaatgaaaag
     6061 ttgtaaatcg atgtgttatc agaaaacata aattgttccc cataaaaaaa ttgacccaaa
     6121 tttattaatc aacacaaaaa aataccggag aaaatcgaca aaaatatttt acaaaaaaca
     6181 gagaattaca ataatttcac cgaaaaagtc aacaaatcat gcagccttca catcgggaaa
     6241 gttcaccgcg aaggcgtttt tctaccaata atttcattct gtctctcaaa gcattgatac
     6301 ggatattgtc catcacatcc gcagtaaagg caaacattag cagcaaacgt gcttcgctct
     6361 ccgaaatacc acgcgattgc atgtaaaact tggctgcttc gtccaattgt ccgatggtag
     6421 cgccatgcga acatttcaca tcgtcggcat agatttcgag ttgcggtttg gttcgcattt
     6481 tggcagtttt gctgaggaga atatttttgt tggtttgaaa ggatgaagtt ttttgggcgt
     6541 tttttgcaac ctgtattttt cctgcaaatg ctcctttgga agaatcatcc aaaacatatt
     6601 taaacaattc gcgactttga gaatttggtt ttttgtgaac aatttcagtg aaattatcca
     6661 catcctgatt tttatcgcca atcaccatgc cacaaagcaa actttcacaa ttttctccat
     6721 caattatcat ttcgatgccg ttgcgtgtca tgccattatg aagagtaatg acgtttgtca
     6781 gtacattcga cgaatcttcc tgatgaatca gtaagtttga aacattggtg ttgaggatat
     6841 tggtgttttc gagtttataa tgttcgtaaa cagcattttt tcctgcaaaa acttcggtga
     6901 gacgattgga aaagaagtgc acattgtcca tagtgtggtc gcaaaccagc aactgagctt
     6961 tggctccttc ttccagaatg attaaattgt ggctgttggc catgaaatcc acgtcggagc
     7021 gcattatgtt tatcaattgt acaggcttgg aaagtttcac gtttttggga acgtacataa
     7081 aaaatccatc ttgagcgaaa gtttcattga aagcaatcca accgtcattt ttttcattgc
     7141 ttaattttcc aagatatttt tctaccagtt gtggataatt ttctgcagct gttcgcagac
     7201 tttcaataat tacgccttct ggcaaatgct gtttggcagt gctgttgacg gggtaaaaag
     7261 tatcatttaa cacaaaaaac aagtaggaat gaatagccgg cacatcacac ttaaaaacat
     7321 cgtaaggatt gatttgaaat tgcaatcggt tgatgttcag tccatattct atcgacaaag
     7381 gtgccgacat atcggtatat ttgtagtttt cgagctttga agtgggaaaa ccgagccgtt
     7441 caaatttctc aaaggcaaca tcgcgaaaat tattcatcaa agcagccgaa tgttgcttta
     7501 tcagggtatg atattcttta taaaaatcga tatattgttg ttccatgatt tttcaaattt
     7561 cggatagtta agaagaattt ttactgctct acaggagttt caacttcctt ttttatccaa
     7621 tcgtaacctt tttcttccag ctcaagtgcc aagtttttat cgcctgtttt tacgattcgt
     7681 ccgtcgtaaa acacatgtac aaaatcggga ataatatagt cgagcaagcg ctgataatgg
     7741 gttatcacaa gtgtggcatt gtcaggccgc ttgagtttgt tcactccgtt ggcaacaatc
     7801 cgcagcgcat caatatcgag tccggaatct gtttcgtcaa gaattgccaa tttgggttcc
     7861 agcattgcca tttgaaatat ttcgtttcgt ttcttttctc cgccggaaaa accttcattc
     7921 acggaacggc tggtcagttt attgtccatt tcgaccaatt ctttcttttc gcgcatcaag
     7981 cgcagaaaat cggcagccga aagaggttcg agatttttgt atttacgttg ttcattgacg
     8041 gcggtacgca tgaagttgac catgctgaca cctggaattt cgaccggata ttgaaaactc
     8101 aggaaaattc cttcgcgcga acgatcttcc ggcgacattt cgagcaaatt tttgccgtta
     8161 aaaataattt cgccgtgtgt tacctccaca gccggattgc cgcacaaaac ccacgccaaa
     8221 gtggattttc cagccccatt cggacccata atggcatgta tttcaccggc acgaattgtt
     8281 aagtttactc cctttaaaat ttcttttccg ttgatggatg catgtaaatc ttttatttcc
     8341 aacataatgt atttttctaa taatctgtaa taaaaatttc tttcaaaagg ttaacaggtt
     8401 ttatcccaca gaaccttcca acgttatctg taataatttt tgtgcttcta cggcaaattc
     8461 cataggcaat ttattgataa cctcccttgc gtaaccattt acaatcaagc caacagcatc
     8521 ttcagtggaa attcctcttt ggttgcagta aaaaatctgg tcttcactga ttttggaagt
     8581 ggtggcttcg tgttccacta tggctgtatc gttgttgacg tccatgtatg gaaaagtatg
     8641 agcaccacat ttatcgccca gcaggaggct gtcgcattgc gaaaaatttc gagcattgtc
     8701 ggcgtttttt gaaactcgca ccaacccgcg atagctgttc tggccgaaac ctgcagatat
     8761 tccttttgag agaatgtggc tgcgggtgtt ttttcccaaa tgaatcattt tggtgccggt
     8821 atcggcttgc tgaaaatgat tggtaaccgc taccgaataa aattcggccg acgaattgtc
     8881 gccctgtaaa atgcaactgg gatatttcca agtaatggcc gagccggttt caacttgtgt
     8941 ccacgaaatt ttggaatttt ctcccttgca aatgccgcgt ttggttacaa aattgtaaat
     9001 tccgcctttc ccttctttgt ctcccggata ccagttttgt acggtagaat attttacttc
     9061 ggcatttttg agtgctacga tttccacaat ggcagcatga agctgatttt cgtcgcgcat
     9121 tggagccgta caaccttcaa gatatgaaac ataactgtcc tcgtcggcaa cgatcagtgt
     9181 tctttcaaac tgaccggtat tggaagcgtt gatgcgaaag taggtggaaa gttccattgg
     9241 acagcgaaca cctttgggaa tgtacacaaa agaaccatcg ctgaaaacgg cactattgag
     9301 cgttgcaaaa aaattgtcgg aataaggaac aactgaaccc agatattttt gaaccaaatc
     9361 gggatgatgt tgcacggctt cgctgaatga acagaaaata atacccaatt ctgccagatt
     9421 ttccttgaac gttgttttta ccgaaatact gtccatcacg gcatcaacgg ccattcccgt
     9481 cagcgttttc tgttcttcga gcggaattcc cagcttgtcg aaagttttca aaatctcagg
     9541 atcaacttcg tccatacttt ttggcgattt ttttcgtggc gccgcaaaat aggatatttt
     9601 ctgaaaatca atgggcggaa tttccaaatg agcccaagtc ggcattttca ttgtcagcca
     9661 atggcgatag gatttgatac gaaaatcgag caaccattcc ggttcgtttt tctttaccga
     9721 aataagtcga ataatttctt cgttaagtcc catcggaaaa acttctgtat cgatgtcggt
     9781 agtgaaaccg tacttgtatt ctccctgtgt aacttgattt attatatctt ctgacataaa
     9841 ttgaaaaatt ttttacttgt caaaaaagtt gcttgtaaat tttagaaatt gtatcagaaa
     9901 ttctaaaatt tcattgtatt tttgataaca tttaaaactc agattttgtt ttggtaaatt
     9961 tcttttttgt agaattaatt gacctccctt ttatcgcaac ttcatgaaaa actttgattt
    10021 ttaatctcaa aaacaaaata agttgaaaac aaaaaccgcc ataccaacat gttatgacgg
    10081 tttttgttaa aattgtagaa gaaaaaagtt ctccttcatc aaaactcttt ttccttgatc
    10141 agatattcag ctatttgaac tgcattcagt gctgctcctt tttttatctg atccgaaacg
    10201 caccagaatg ttattccgtt gggatttgcc aaatccttgc ggatacgtcc tacatataca
    10261 ggatctttac ccgacaggaa aagtggcatg ggatataatt tttcagccgg attatccatc
    10321 accaccacac cttcggcatt ttcgaaagca gctttcactt cttccaagct cagaggacgc
    10381 tcagtttcgg cccagatgct ttcggaatgc gcacgcaatg aaggcacgcg cacacaggta
    10441 gcactcacca ctacgtcgga atgcatgatt tttctggttt cgttgaacat tttcatttct
    10501 tcttttgtat agccgttttc agtaaacaca tcaatttgag gaattacatt gaaagccagc
    10561 tgataaggaa atttattcac tgtcggtgtt tcaccagcca atacctgacg gtactgcagt
    10621 tccaattcgg ccattgcagc ggcacctgca ccacttgcag cctgatacgt ggcaacatga
    10681 acgcgtttga tgtgtgaaac gttttcaatg gcttttaaag ctacaaccat ttgaatggta
    10741 gtgcagttgg gattggcaat gatgccgcgg ggacgatttt ttgcatcgga agcatttact
    10801 tcaggaacta ccaacggcac gtcattttcc atccgaaaag cagatgaatt gtcgatcatc
    10861 aatgccccgt atttggtgat atcttcggca aattctttgg atattccggc tcctgcagag
    10921 gtaaatgcca agtctatgtc tttaaaatcg tcgttatgtt ttaattcctt tacggtaagt
    10981 ttttctcctt taaaatcata tacgctgccc gcactacggg aagagccaaa caggctcaag
    11041 gaagtcaatg ggaaattgcg ttcggctaaa acgcgcaaaa attcctggcc aacagctccg
    11101 ctggcgccta caattgctac tctcattgtt gtaaaatttt ggttatttgt atgtttggta
    11161 aaaagaaata tgcacgcaaa taattttctt tgttaagaaa tttttatttt tgcagtatat
    11221 tgtcttaaaa aattgattct tcttcttttt tcggtacaaa attaatctaa atcatagaat
    11281 tttcataaaa aatgaagaaa acctgtctta tttttatgta tttactgcct gtcttttctt
    11341 tcggacaagt acttgacgac ttttctgatg ggaattttac tgaaaatcca tcatggagcg
    11401 gaacgacagg aaattttaag ataaacgaat attttcagtt gcaatccaat gctccaacaa
    11461 cttccgtttc ctatttgttt acgccatcgg aagtttttta cgatgccgaa tggcaagcat
    11521 ggtttaaaat taattattcc acttcttctt ccaattattc cgctttttac attatttcga
    11581 gttctcccga taatttagga aatgcttatt ttgtaaaaat tggtgatacg caggacgatg
    11641 tttcgttgtg gagacagcaa ggaacgtcaa aaatcaaaat cattgatggc acagataagc
    11701 gcacagatgg tacacaggtt gaaattcggg taaaagttac ccgcgattca ctgggttact
    11761 tttctcttta cagtaaactc ccttccgaaa gtgattttgt gctggaagga acggttcgcg
    11821 atacaactat cgaaagcagc tcatatattg gtttgcttta ttccaataca acgactacaa
    11881 cctcagctta ttttttcgat gatattgctg ctaacgggaa aaaagctgtg gataaaaaac
    11941 cacctgtttg ggaaaaactt gccataattg aacctgatac cttgcagttg caattttctg
    12001 aaccggttga cttgtccaat tcaattttca ttttgaacga aaatgaaatt actcctacaa
    12061 aaatcgatat tttatcaacc aaaccttttt tgatgaatgt tgcgctgaac gaaaattttg
    12121 ataagggaaa aatttatacg cttgaacttt tgcaagtaac cgatttggca ggaaatacgt
    12181 tacttgacaa tatcaaaaaa accggtatta ttgaaccttt agcgcaggga gatttattgc
    12241 tgaacgaagt aatgtttaat gcacctccta ctgctactga atacatcgaa atctacaatg
    12301 cttcagccaa aacgctggat gtttcgggaa tagtcataac tacacgcaaa tcggatggtt
    12361 cattgaatac tttgcgccat attcctgacg gtaaattgtt ggcagccgga ggctatttgg
    12421 ctgtatgtca gcatgccgat tcggtaatga attattttgg cctgccaaac gatggcagta
    12481 tcgtttccat ttcgtggacg gcactcaaca atcagcaaag taccattgtg cttgccaatt
    12541 ccaaagccga tactgtatat gatgaattga cgtacaatga aaaatggcat cacctaatga
    12601 taaaaaaccg gcagggtgtg gcattggagc gaattaatcc ggctctgccc acacaatcgg
    12661 cggaaagttg gcactcggca gcctctgaag ttaaatacgg aacacccggc tataaaaatt
    12721 cacaatatcg tgaaatcgaa aacacttttt caaacaaaca attcgtttgg gttgaacccg
    12781 aagctttttc tcccgataac gatggtacgg atgatgtctg tttcataaaa tatcgtactc
    12841 cgacagaagg ttttgtggct aacgctcaaa ttattaatgc gaccgggaca aaagtttttc
    12901 agttggttag caatgcgttg ctatcctcag aaggtttctt ttcttgggat ggccgcaccg
    12961 atagcgggaa aattgctaat gcaggaattt atgtactcta ttttgagatg attcaaccaa
    13021 aaaccggcca aaaaaagcag atgaaaattc caattgtggt ctctgcacga taattttgaa
    13081 aaattaacat gttggaatgc aataaatcag ttatctttgc tgaaattttt aaagttttaa
    13141 tattctaaaa atcaattatt cactataaaa atgagaaaaa tatttgtttt atctttattg
    13201 gcaattttct tgataacttc ggcagaggcc aaaaagccca aaaaaattca agaagaacct
    13261 ccggttcaga aactcaccaa cactatcgat tccatgagtt atgctttggg tttgagcatt
    13321 ggatctgatt ttattaaaaa tttgaaaacc attccgggtg gtcaatttaa tattgattgg
    13381 ttgatacagg gatttaccgg cggtatgaaa aaagattcta ctgtaatgac cactgctttt
    13441 gccaacaatt attttcgtga atacattact aaagctcaat ccttagagtc ggcaaagaaa
    13501 aaagaagttg ctgatgcttt tatggaaaaa aacaaaacca gagaaagtgt aataacaact
    13561 gaaagtggtt tgcagtacga aatccttgca tgtgccaatg gtccaaaacc atctatcaat
    13621 gatacggttg aggttaatta tcaaggtttc cttcccgata gtaccaagtt cgacagtact
    13681 tttgatcgag gagaacctgc agtttttgct ctcaatcagg taattccggg atggagtgaa
    13741 ggcctgcaac tgatgaccgt cggttccaaa tataaatttt atattccata tgagcttgct
    13801 tacggcgagc agggtgccgg aggcgttatt cctccttatt ctccactgat tttcgaagtg
    13861 gaattattgc aaataaatcc agctaaaaaa taaactaaaa atttttgtat cataaaaaaa
    13921 taagtcagtc aaaatgaaaa aacaaatcgg ttttatgttg gcaacagttc ttgccgtttt
    13981 tttgttggct tcttgtagta aaaaatacga agcaaaaaaa gtccaattaa aaactcaaaa
    14041 tgacagtctc aattacacgt tgggtcttgc caatggagaa ggaattaaga attactattt
    14101 acaaaacgac tcatcggatg ctcccattaa agctttgata aatgctttgg acaaagctta
    14161 ttccagtaaa gaagtagatg atcagatgta tcagttgggt tatcagattg gaacttctct
    14221 gaagtatcag aaagaaaatg ggttgatggg tgattccacg ttggttttca ataccaagtt
    14281 gtttaaactc ggtcttgtca acggacttca cggtttcgat ggtggaatga cacctcaaca
    14341 agctcaggag tatttacaaa aaacaatgac cgagttacaa gcaaaaaaat cggctcaaca
    14401 agcacctgct aatcagcctc aaccggctcc agaaagtcaa aaaactgaca gtgtgcagta
    14461 attcaaatga attaagacat tacaataaaa tctcatataa tttttgtaag atgaaaaaat
    14521 taaatttatt tcttctggta gccatttcag ctactttcac tttttcttca tgtaacagcg
    14581 ataaacccgg caagccgact cttaaatcgc aaatcgacag tttgaattat gcttttggcg
    14641 ttgccaatgg tgatggaatc aaaaattatt atttacaggg cgattctacc ggcaaagctt
    14701 taaattcttt gttaaaagga atcaacgatg gtttaaaatc caaagtggac aaagaaaaca
    14761 tggaacttgc cagtattggt tctcaaattg gatactctct taaagaacag agcaaaagtg
    14821 gtttaatggg cgactcttct ttatctgtca atatcaattt ggttaaacaa ggtttaatca
    14881 atggcatgaa aggctcgaac atccaaatga ctcctcagga agccagcgaa tatttgcaat
    14941 ctaccatgca aagtcttcag gaaaagaaaa tagaaaaaca atatagtgcc aacaaggaag
    15001 ccggtgaaaa gtttttgctt gaaaatgcaa aaaaatcggg agtaataact acagaaagtg
    15061 gtttacaata cgaaatcatc aaaaaaggca aaggtcctat cccaaccgac agcagcaaag
    15121 tcaaagttca ttatcatggg acgttgattg atggaactgt tttcgacagc tctgttgaaa
    15181 gaaatcaacc tgccgaattt tatgtcaatc aggtgattaa aggttggact gaagcattga
    15241 aattgatgcc ggtaggttcc aaatttaaaa tttatgttcc ttaccaattg gcttacggaa
    15301 gtgccaatca aggtgccata aaaccttttt ccatgctgat ttttgaagtt gaattacttg
    15361 cgatcgaaaa gtaatttttc ctaaatttta ttcttacaac atcccttgtt tcttaaaatt
    15421 agaaacaggg attttttttt tgaacatata taatacagaa atactttaaa gcacagcaaa
    15481 aagaattttc atgtggtttt ggtttgattt aggaaaagta agttatactt tttgaggttt
    15541 tgattatcct tttcatcaat cgaatcgaaa atgatgcttg caggtcaaac caaaactaaa
    15601 caatggctaa caggcataga tatggtttgc atgcaaaatg atgtaaagga agtaattgcg
    15661 aattataaaa aaggaaaaat aattgttgcg aggatttaat ctactttatg cctctctttt
    15721 acatgaagag cctaaaacta ttattctaac ctgtgaaata agcaaaaaat ttttcattac
    15781 ctaaaaagga gagcatttca tagttcccct ctcctatcga actctgattt tttgaccggg
    15841 ttttaataac tttttagatg atatctgatt taaattgcaa agttgtttta ccgttgtgcc
    15901 atagcgtgaa gctataagac taagtgtatc acccttatga acaacatggt agcgtgccat
    15961 ttgaagactt ttcaaatctt tttgatattt gaaagtattt ttcttggtaa tcaggtaaga
    16021 aaattgtctt atggaacctt tttcaaagtc aaccaaatta tcaggatcaa tggcgttccc
    16081 caaataacgc atttcaaaat gtaaatgtgg tcctgtggaa cgaccggtat ttcctcctaa
    16141 agctattacc tctccagctc caattatttg attcagttct acgagaactt ttgaaagatg
    16201 agcataaacc gtttccagtc cgttattgtg tctaactact acataatgac cataaccacc
    16261 atgttcataa tcaataatgc gtacctttcc ttcaaaggca caacgaactg aatcgcctac
    16321 atttagtttt aagtcgattc cgtaatgata acgatatcga cggcggccaa atttagacgt
    16381 tacttttccg taaataggta gaatgaaatt ggaacagtca attcttacgg aatcaggcag
    16441 actatctacc ggaaaatgat agggatttaa cctttcggaa gtccaaatgt tgttgtaaat
    16501 atcgtctgcc gggtgatttt ccatcaaatc atcagatgtg tcattcaaca tttggtggta
    16561 aatattaaga gtatctttta aatttgaagt tgtcgagttg ggaatattaa ctttttctgc
    16621 gtttattgca ttgcaaaaca acaaaaaacc aagcaataaa aaaaagaatt ttaaacactt
    16681 cgaaatatac tccatcatca aaacttttct tttaataaaa aaattaaact tcatcattag
    16741 cataaacaaa aggaaaatta ttgggcttaa actcaaaaat ttcttcaggt ttgaaaaaac
    16801 ggtcaatttc taccttggca ttttctaccg tatcggaagc atgaacaata ttctcctgca
    16861 cgctcatgct gaaatcgccc cttattgttc cgggagctgc ttcacgtcca ttggttacac
    16921 ctgacatgga acgcacaatt ctaacggcgt ccagtccttc ccaacaacaa acaatcacgg
    16981 gcgtaaccat cattgaatcc ttgatacgct taaaaaaagg ccgatcggcc aaatgagcat
    17041 aatgttcaga aaccactttg tcggtaaact gatacatttt tattccgcac aaatgcaatc
    17101 ctttcctttc aaatcgggta atgatttcac cgatcagttc gcgctgaatg gcaccgggct
    17161 taataataac aagcgttttt tccattaatt taaattgtaa tgaaaggaag ttttatatac
    17221 aggagatttt tggtttccca aaaataaaaa aaaatgaatc aaaaacaaaa ccttattagt
    17281 tttttttaac taataacgct ccaatttaag gatgaggttt tcattttttt taattgttca
    17341 gagagtatta aattttcttt tttctgcaac tgcgggtctt cttctataat atccatagct
    17401 acttttcgtg ccatttcgag catttttcca tcctgtgcaa gattggcaat tttcaactcg
    17461 aaaggtaatc cgctttgctg tgtcccgtcc aaatcgcccg gtccccgtaa ttgcaaatcg
    17521 gtttctgcta tctgaaaacc atcattggtg ttcaccatag tttcaattct tttgcgggtc
    17581 aaattgctca atttgtatcc cgtcagcaaa atgcagtacg actggtcagc tcctcttccc
    17641 actctgcctc tgagttgatg caactgagcc aaaccaaagc gctcggcatt ttcaataatc
    17701 attaccgaag cattgggaac attcacccca acttcaatca ctgtagtagc aaccattatt
    17761 tgggtttttc cggcaacaaa tttctgcatt tcggcttcct tttcggcagg tttcatttta
    17821 ccgtgtacca tactgatttg gtattgcgga aaaatttcgc tcaaatgttg gtaaccttcc
    17881 tccagatttt ttaaatccat agtttcagat tcctgaatca acggataaac aacataaatc
    17941 tgtcttccgg cttcaatttg tttcaccata aatttataaa gcgcctgcct tttgttgtcg
    18001 tagaaatgaa aagtctgaac aggttttctg ccgggcggca attcatcgat aacggaaaca
    18061 gtaaggtcgc cgtaaatggt catggccagg gtacggggaa ttggtgtggc tgtcattacc
    18121 agaatatgcg gaggttgaac atttttactc caaagtcttg cacgctgagc cacgccaaaa
    18181 cgatgctgtt cgtcaatcac aaccaacccc agatttttga actgaaccgt ttcttctatc
    18241 aaggcatgtg tacccagcag aatattcaat tcaccgctca gcagcttttc gtgaatcgcc
    18301 gctcgttcct ttggtcgcgt cgaacccgtg agcaaagcaa tttggatacc aatatttccc
    18361 aaaaactgct tcaaactttc aaaatgttga tgggcaagaa tttcggtggg agccatcata
    18421 caagcctgaa aaccattatc aaccgccatc agcatggcaa aaagtgctac cagcgttttc
    18481 ccgcttccca catctccttg aagcaatctg ttcatttgtt tgccggaagc aacatcggag
    18541 cgaatttctc gaatgacttt tttttgggca ttggtcaact caaaaggcaa ataatcgcga
    18601 tagaaagtat tgaaataata gccaacatgt ttgaaaacaa accctttaaa ccgctgattt
    18661 cgccatgcag tttgtttcac aatatccaac tgaatgtaaa aaagttcttc aaatttcagt
    18721 cgctgtcgtg cgcgattcaa caaatgactg ttttttggaa aatgtatgtt gactaaggca
    18781 tctttcaggt taagcaatcc ataacgattc agaatgtatg ccggcaaggt ttcaggaatt
    18841 ccattttgca aagtcggcaa cagtgaataa ataattttct gaatggcttt ggaattcaga
    18901 aaatgagtct tcattttttc ggaagtattg tagaaaggct gtaagcccat ttgctcaacc
    18961 gggggaagtt gtgaaagaat ttcgatgtcg ggatgaacta tattgtactt tccgttgaaa
    19021 agcgtaggtt ttccgaaaac aacatattcg atattggttt tataacgttc tgctacaaat
    19081 ttcaaacctt taaaccacac cagctcgata tattgccttc catcggtaaa taccccaatc
    19141 aaacgctgac tgcatccttc accaaccttc tcaaacttca gaattttccc tttcagttgg
    19201 atgaaaggca tattttcgga aatttcgctg atcatgtaaa aacgacttcg gtcaacgtat
    19261 ttataaggat aatgtctcag caaatcctca gccgttcgga tgttcaattc ctttttgaaa
    19321 agctcggccc gcttggggcc aacgcccgga ataaattgta tgtcttgggt aaaaaaatcc
    19381 acttatgtaa aaaatcttca aaaaattttc caaatcaata actgctgttt tctcttttcg
    19441 ccaacagaaa agatgccaat tccaaatcaa aagctgtagt cagtttaata ttttcccgat
    19501 ttccttcggt gagatgaaca tttacaccga tagcttcaac cacagaagca tcgtcggtga
    19561 aaagtggaga aaactcctgc tcataagcct ttttcaaaat gttaaaatca aaaacctgcg
    19621 gcgtttgcac cagtttgtag gcacttctgt ccaacgattg gctgtacttg gtatgaattg
    19681 tgcgaaggct atcaaccaaa tccatactcg gtatggccga accaaatttt tcggcttcgt
    19741 caaaacaacg ccgaatggtc tccacactta ccaaaggccg aacaccatcg tgcacagcaa
    19801 ccaatgctga attattctct tcctcagatt tatccggata aagcgacgct aaaaaagcag
    19861 caacagcttc caacccgttt tttacggaat gaaaacgcgt ttcgccacca ataaccactt
    19921 ggtgagaaaa tttgaaagaa tatttcgaac aaagattttt ccaaaattca atttgagtta
    19981 caggcaaaac cacaaataca ggtaaatcga aatcgtaacg tgcaaaagct tcaatggtgt
    20041 gcatcaatat gggttttccc gctatttcaa taaactgttt tggaatgtcg gactgcatgc
    20101 gttcaccttt tccgccggca acaataagca caatttttgt aagttccata aattgggaaa
    20161 aatagttacg aacaaagata aacattaaac ttcaaaaaat gatgaccaaa tttagttcaa
    20221 tctttccatt atttcataat ttcgtcgaaa agctatcgga tttacccgat attttataac
    20281 tttgcactcc aatcatgaat gaaatgatga aaaattttaa atttttactt ttcacaattt
    20341 ttctcgcagt gtctggcggg cttcaggctc aggatgaaag cacatctgtt cgacagaata
    20401 tttttgaagt tcttcaagca tcggatggag caggcaaagt tatcattcat caagacaagc
    20461 gcatagaaaa tcttatcaat caaaagcaaa ccgccaaaac gggtcgtgaa gcgacgttgg
    20521 ggaatggtta ccgcgtgcag gttttttcga gcaacgaaca gcgcaccgga aaagttgaag
    20581 ctttcaaaat agaaaaagaa atacgtgagg ctttccccaa taccggcgtt tatgtcaact
    20641 acaactcgcc tttctggaaa gttcgcgtag gcgacttcag ttcggtagat gaagcgcaag
    20701 cctttcgcaa caagctgctg gaagtctttc ccgacctgcg aagtcagact tatgtggtga
    20761 gagatcagat tgtaaaatag ctttttgaaa aaaaatcagt ttcatggatt tcgacattac
    20821 cagttcccgt ccttttgacg aaaaaaagac aggagaaatt gcacaacatt atcaggaaat
    20881 tatccgtctg ctgggcgaaa accccgaccg tcagggtttg ctgcaaacgc ctcagagagt
    20941 ggcacgcgcc atgcagtttt tcctacaagg ttacgaacag gacgctgaag aaattatacg
    21001 cacggccact ttcacagaaa actaccgtca gatggttatt gttaaggata tagattttta
    21061 ttcgctttgc gaacatcatt tgcttccttt tttcggaaaa gcgcatgttg cctatatccc
    21121 caacaaaaaa attaccggtt tgagcaaaat agcacgcgta gtagaaattt atgcccgtcg
    21181 attgcaagta caggaacgta tgaccacaca aataaaacaa tgtattcaaa acacgctcga
    21241 tcctatgggg gtaatggtta ttatcgaagc gcagcatctg tgcatgcaga tgagaggaat
    21301 acaaaaacaa aattctatta caaccacttc cgattttaca ggcgtttttc aggaagcgcg
    21361 cacacgtgag gaatttatga atttgattaa ccgatagccc gaaatcataa aacatcaact
    21421 ttcccctaca cgcagcaaca tacaattaaa agctatattt aaaagtcttt ttgtaagttt
    21481 tttaaaaaca agacaaaagt ttaaacaatc tttttaaatg ttcctttttc ccaaagacaa
    21541 atcaaattag agtccgctcc gaaaagtcaa aaaagctaaa ttcagcaact ttttgccctt
    21601 aatttaaatg taggttgctg aaaaatgagt tttctttatc gtttgggcga agcgaatttt
    21661 caaaaataaa acttttcgga gcggactcaa attatattta tccgcaatgc gaatatccac
    21721 agtttttgca ggttaaacaa ccttcttgat agatcagtgt tttttgtcca caggaaggac
    21781 aatttttacc attagcttct gttccatccg gaatatattt tttcagggca cgttctactc
    21841 caactttcca agtgttgatt gattcgctgt ccaactgcag tcccgaaacc aattttatta
    21901 cctggtcaat cggcattccg taacgcagca cgccagaaat taatttggca taattccaaa
    21961 attccttgtc aaacttatgg gacaagccct caatggttgt tttataccct cttttgttgg
    22021 taaactgaaa atcgtatctt ttgttgccgt tttcgtccac cgttttaatt atttttccct
    22081 gagtaacagt tttaggcaat gcaattcctt cttcgtcatc agccaatccg gtaaaaattt
    22141 cataaggacg cccatctttc agccctacaa aagcaatcca tttatctttg gcattctgaa
    22201 atctgaccac atcgcaatcc aattctttcg gacgaattaa atgctcttcc gactgaggaa
    22261 agcaaatttt tctctcgcct gatttctcgg attttttttc ttctaccgac agcaaaacac
    22321 cggctcgtga accttcacga taaacggtta ctcccttaca tccgcaacgc catgcttctt
    22381 cataaagttg accaaccagc tcttcggttg tatcggcagg taaattgatg gttacgctaa
    22441 tggaatggtc cacccatttt tgaattcgtc cctgcatctg aacttttttc aaccagtcaa
    22501 tatcgttggc tgttgctttg taatagggag atttttccac caattcgtca agttctgctt
    22561 tggtatactt ctgggtagtg gaataatgat tggcttccat ccatgtaaca aacttatgat
    22621 gaaaaaccac atattcttcc catgaatcgc cattttcgtc cacaaaatct acgcgaacat
    22681 ttttatcatt gggattcact tttcgacgac gggtataaac cggcataaaa accggttcga
    22741 tacctgaagt tgtctgtgtc atgatgctgg ttgttccggt tggagcaatg gtaaggcagg
    22801 caatgtttct tcttccatgt tttaccattt catcgtacag ttcaggatcg gcttcacgta
    22861 aacggagaat atagggattt ttttcttcgc gttttgcatc ataaatgctg aaagcaccgc
    22921 gttccttggc aagagtcaca gaagaacgat aagccgccaa agcaagaatt ttgtgtacct
    22981 gttcagaaaa atctatggca gaattggttc cataacgtaa acccaacgca gccaacatgt
    23041 ctccttcagc tgtggtaccc acaccggtac gccgacccat agaagtttta tttttaattt
    23101 tttcccaaag tttgagctcc gtgcgtttga tttcttcatc ttcgggatcg gcatttattt
    23161 tggccataat tttatcgatt ttctccattt ccagatcaat aatatcgtcc ataattcgct
    23221 gagctactgc gacatgcttc ttaaacaaat cgaaatcgaa tgaagcttcc ggcgtaaatg
    23281 gatgattgac atacgaatac agattaatag ccagcagtcg gcagctatcg tatccgcaca
    23341 gtggaatttc gccacaagga ttggtagaaa tagttttgaa acccaaatcg gtataacagt
    23401 cgggaacaga ttcgcgggta atggtatccc agaaaagtac acccggttcg gctgattgcc
    23461 aagcattgtg gataattttt ttccacaggg aatgggcatc tatttctttt ttgaaccacg
    23521 gatggtcatc atctatggga aattgctgag tataagtttc ctgcgctaaa gcagcctgca
    23581 taaaatcatc gtgaattttc accgaaatat tggcacccgt aattttccct gcttccaatt
    23641 ttgcatcgat aaacgattcc gaatcgggat gtttaatgga aaccgtcaac atcaaagcac
    23701 cacgacgacc atcctgtgca acttcacgag tagaattgga ataacgctcc ataaaaggta
    23761 caattcccgt agaagtaagc gcggaatttt tcaccggcga acctttgggg cgaatgtgag
    23821 aaagatcatg ccccactcca ccccgacgct tcatcaattg aacctgttct tcatcaattt
    23881 taatgatacc gccataagaa tcggcatcat ggtcaaaacc aatgacaaaa caattggaaa
    23941 gtgaagctat ttgaaaatcg tttccaattc cggtcatggg gcttccttgc ggaacgatat
    24001 acttgaaatg ccggaaaagt tcaaaaagtt catcttctgt cagcggattg ggatattgtt
    24061 tctcgattct ggcaatttca cgagccaatc ttttgtgcat gtcatccggc gtcaactcgt
    24121 aaaaattccc gtaagaatct tttaacgcat attttgttga ccaaacagtg gcagccaatt
    24181 catctccctg aaaatattca aaactggctt tttttacttc ttcggcagta taagtttttg
    24241 gtttcacaac aaaaattttt aattgggtta ataaataaag gtttaagaaa aattagcgac
    24301 gcatttttta caactgcaag tttataaata aaaaaatgat tatccaataa tttcaataat
    24361 tatttttcct tttcggtaaa aattttctat ttcaaaaatt atattattga ttattaataa
    24421 tttaactttt tatattaaat aaaaaatttc cttatttgaa aacttttgca tataattaat
    24481 tttcaataaa ggaaatctat aacctaataa tataaataga aaaaagatag atgagattaa
    24541 taaaacttac aaaattattc cattctctac cttaaaaatt ttggcttcct gatcgagttg
    24601 gtaaagaatt tgatccaaat gttcgcgatt ggtgtcggta ataaaaatct gaccgaaagt
    24661 ttcctgtgaa acaagttcaa caatttttct aacacgatcg gcatcgagtt tgtcaaaaat
    24721 atcatccaat aaaagtatcg gtggttgatg atgggtttgt ttaagaaatt tgaattgagc
    24781 caatttcaac gaaaccaaaa aagttttatt ttgtccttgt gaacccacac gcttaatcgg
    24841 ataaccattc aacaacattt ccaactcatc cttatgaatg cctcgtgtgg aaaaaccaag
    24901 cattttatcc cgttcacggg ttgacttcat ttgttttttt atatcgtttt gctgaaaatg
    24961 cgacaaataa gtgatgtcaa tctcctcatt ttcatgggaa gaaatatagc gatagtactg
    25021 ttgaaacaca ggaatcaact gatcgataaa acgggttcgt tgttcaaaaa tgtaagaacc
    25081 atagtcggcc atttgttctt cgagaatttc aaacaaagat tcgtcgacgg gcgtttcgac
    25141 cttcaacaag gcatttcgct gcttcagcgc attgttgtaa agcaataaat tatgcagata
    25201 ttttttgtcg tattgcgaaa tcacgccatc aacaaacttt cttctctcgt cgctgccttc
    25261 ggaaataagg accgaatctt cgggcgaaat caataccagc ggtaacaatc caatgtggtc
    25321 ggatagacgt tcgtagtcct ttttgttgcg tttaaattgc tttttctgat tgcgtttcat
    25381 tccacaataa atctcctctg ttgcttcgcc catacgatat tttgcctgca atacggcaaa
    25441 atcggcctca tgaaaaatat tttgtgaatc aataaagttg gaatgacttt tacaaaaaga
    25501 caaaaaatat acggcatcca gcaaattggt tttccccatc ccgttgttgc caataaagca
    25561 attgattttc ggggaaaagc tcaattcagc ctgacggatg tttttataat tgacaatgtt
    25621 aatcgattcg atgtacatga atgctaaatc aaaactttgt gttcacaaat ttacgggata
    25681 atttttgaat ttcacacggt aaaaaacaaa aatacatgaa taacgctgac gtcggcttct
    25741 ttagcatgaa atttcgaaat tcaggggatt aaaccaggat taagctttga caatttaaaa
    25801 aaggaaagtc aagctcagaa gagctgtccc atattcatca aaaccatgct atcccttttt
    25861 ggtaaaaagc aattttgcat caatgtttat tatgaatcat ttcaaaacgc atccggaggc
    25921 caaaagtatt tatcgcataa aaaacttttg attcgcaaac aggtaattgg ttagaccaaa
    25981 ccgaacgaaa tagaaaggat accatcagag aataaggagg ctgcagaaat tattggtttg
    26041 tgtataattt aaatctaaaa attctgcaag cctgtttgga aatggaaatt atgaatgatg
    26101 agcggcaaaa cgattgaaaa taattaaaaa tgctcgacca tttttcctat gatcgagcaa
    26161 ttgtttagaa agaaaaaatt attaaatacg ttcgagattt tcggttgtaa ttttttcttc
    26221 tacaatggca tcaagaacag cgatagcagt catattgaca atatcgcgga cggagctttc
    26281 cacatccaaa atatgtacgg gcttattcag tcccatttga attggcccaa cagcttccga
    26341 gattcccatt tcgagcaata gcttgtaagc agaatttgca gaacttaaat tggggaaaat
    26401 caatgtattg acatctttcc ctgctaatct ggtaaaagga tacttgcggt cgcgcaattc
    26461 tttgttcagt gcaaaattca cttgcatttc accgtcaact atcatttcag gtacttcgcg
    26521 gtgcaatgtt tcaactactt cgtgaacgct ggctggactt ccctgtttgt cggaaccaaa
    26581 attggagtag gaaagcatgg ccatcaccgg ttcgtgtgca aagaatttta ccgtacggta
    26641 agttaatttg gcaatatcga ttaaagtttc ggtagaagga tgacggttaa acaatgtgtc
    26701 ggccatgaaa aaagttcctt tttttgtatt cataatgtgc atggctgcaa tatgtttgga
    26761 cccaggcctg atgccgataa cgtcctttgc tgcctgaatg gcatcggcat attttgtata
    26821 aactccagtg ataagggcat ccgcttctcc ggtttcaacc atcatcatgc cgaaataatt
    26881 gcgttcatac attttttcat aagcttcatc gtaagtcatt ccttcgcgtc ccagtttttt
    26941 tgcaagtttt tcggcatagg caatacgtct tgcttcttct ctgtcgtggc ggagattaat
    27001 tatctcgatt cctgacaagt cgatgttgta ttcgcgggct atgctgttga ttttttcttc
    27061 attacccaac agaacaggga aacaaacacc ttcgttgtaa gccatttcgg cagctttaag
    27121 catattcaga tggtttgctt cggaaaatac gacccgtttt ggattttgac gtgctgtttc
    27181 gtaaaagctt cgcagcattt tatcgtcata acccatcgtt tcacggagtt ttgcattata
    27241 gccggcccag tcggtaatgt gtttgcgtgc aacccccgat tccatcgctg ctcgagctac
    27301 agccggagcc acaatggtaa gtaagcgggg atcgagaggt ttgggaataa tataatcgcg
    27361 tccaaaactt aatcttttca agttgtaggc agcattcacc acgtcaggaa ctggtttttt
    27421 tgccaattca gcaagtgcat aaacggcagc caatttcatc tcctcattga tgcaggttgc
    27481 cctcacatca agtgccccac ggaaaatata aggaaatccg agtacgttgt ttatttgatt
    27541 gggatgatcg gaacgaccgg ttgcaaaaat aatatcttcg cgtgccgaga tagcatcttc
    27601 ataagatatt tcaggatttg gattggctaa ggcaaaaaca atgggatttt cattcattga
    27661 tcttaccatt tcaatagaca gaacgccggc aaccgataaa cctaaaaata catctgctcc
    27721 ctttacagct tcttccagtg tattaatttc tctgtcggta gcaaaggctt ttttcgtttc
    27781 attcaggtcg gtgcgttgtt tgttaattac tccttttgag tcgagcatga caatattttc
    27841 aggtttagcg cccaatctga tatataattt tgtgcaggaa acagctgcgg ctccggctcc
    27901 attaacgacg attttaacgt cttccattct tttgccggca atttcaagtg cattaatcag
    27961 tccggcgccc gaaataatgg ctgttccgtg ctgatcgtcg tgcataatgg gaatatccag
    28021 ttctgctttt aatcgttctt ctatcatgaa acattccgga gctttaatat cttccaggtt
    28081 gataccgcca aaagtaggtg agatagcttt tactgcctga ataaattttt cgggatcttt
    28141 ttcgtttact tcaatgtcga aaacatcaat accggcgaaa attttaaaca gcaaagcttt
    28201 tccttccata accggtttcc ctgccatcgc gccaatatcg cccagcccca atacggctgt
    28261 accgttggaa attactgcta ccaaattacc tttgtttgtg taatcgtaaa cagtagcggg
    28321 atctttttct atttccaaac aaggctccgc tacgcccggc gaataggcaa gcgataaatc
    28381 tctttgagaa ctgtgaggct tggtaggaac aacttcgatt tttcccggtt ttccttgcga
    28441 atgataaagg agcgcatctt ctttggttaa tttggtcata tagttttttg aatcaattag
    28501 aaattaaaaa atattaatgg gtttgtttta aaaattgtcg taaaattaca tctttttatg
    28561 acgatgtgca attataaact taactctttc tatattttcc gaaaagaact gcttcctttt
    28621 aaggaaaaat aaattttctt aacccacttt ctgtattttg tctgaactta tcgtaaaaaa
    28681 attttatatc aagccttttt gccaatctat ggaattagtt ttttaaaatt gaaacaattt
    28741 taactgtata ctgtttataa ttcagcttta attcactccc ttcgattttt ttttcgaaat
    28801 ttattgacaa gaactttata tttttaaatt tttaaactta tctttgcatt gcttttgact
    28861 gtgcacagga aataacctta caaaaattta tccttttggt tttttatgaa agagatattc
    28921 aaatttttaa acaatacgga tacgttctct cacctggctt ctttaaaata tttacctcgc
    28981 tgggtcgttt tatttataga tattacaata tctgccatat cttttttagc agcttatctc
    29041 gttgcttcat ctttcataaa acacttgcct gaccaacttc ttttacctct ttttccacgt
    29101 ttgattttat tgctttttac acaaagtgtt tttttctggc tttttcatac ttattccggt
    29161 atcctcagat attctacaat gacagatgct gccaaacttt ttttggcaat atttggtaat
    29221 tgtattttcc tctcagcgtt gaactttgtt tgttctcttt attttcctgc accacttttt
    29281 tattatgcat ccattctgat ttatggtgtt attagtttta ttctgttatt cggtctcagg
    29341 ctcaccgtaa gaaatatttt caattatttt gttgaaacct cggctcagat cattccggtg
    29401 atgatttatg gcatcgatga ggcgggtctg ggtatagcca gaatgattct ttccagtgtg
    29461 aattctaaaa aacaatataa attggttggt ttcattgatg gcaatgaaaa tattgtcaac
    29521 aaaatggtaa tgggattgag agtgtataaa catgatgatc atactattaa aactaaagtt
    29581 gccaataaag taaataccgt tattgtttct cccataaaaa tgaaggaaat taatccggat
    29641 gtggatttgg atatatttat tcagcatcat atttcattgc taacatctca gccgatggat
    29701 atttgggaag gagaaatgcc ttccgtgaga caaatcaaac cgttgaaaat agaagattta
    29761 ttggagagag aaccgattca gatttctttt gacaatattg ctgccgaatt gaaatcgaaa
    29821 actgtgatgg ttaccggagc agcaggttcc attggcagtg aaattgtccg tcagttgtgt
    29881 aattttactc caaagttaat tgtgttggta gataatggag aaacggccat tcataacctg
    29941 aaaatcgaat tgcaagcctt ttctacatct caacatttta attttgtatt gggtgatgtt
    30001 cggaataaag agcggatgga gtatattatg gatttatatc gtcccgattt tgtatttcat
    30061 gctgcagcct acaaacatgt tccaatgatg gaagattttc cctgtgaaag tgttcaggtg
    30121 aatgtgctgg gcaccaaaat tatggccgat ttggcattga agtatagagt ttcacggttt
    30181 gtcatggttt ctaccgataa agcagtgaat ccgaccaatg tgatgggagc ttccaaacgt
    30241 atcgccgaaa tttacgtgca atcattggcc agaaaaatgc agaaaaccgg aactgaaacc
    30301 acaaaattta ttactacgcg ttttggaaac gtattgggat cgaatgggtc agtggttccg
    30361 ttttttcaaa aacaaatcga aaaaggagga cccgttacag taacacatcc tgatattatt
    30421 cgttatttca tgactgttcc cgaagcaagc agcttggtgt tggaagcggg tgtaatgggc
    30481 gaaggcggag aaatttatgt atttgatatg ggaaatccgg taaatatcag tcatctggca
    30541 aagaaaatga ttcaaatggc cggattaatt cccggtgtgg atattcagat agcttacacc
    30601 ggcctgcgtc cgggtgaaaa attatacgaa gaacttttaa gtcataaaga aaaagtgaga
    30661 tctacccaac atccaaaaat tatggttgct tcggttgcag aatacgattt tgataaaatc
    30721 tccgttgata tagataactt gattaaatac agctatctct gcaaggattt tctggttgta
    30781 tccatgatga agcagattgt cccggagttc aaaagtaata attctcaata cgaattattg
    30841 gatatcagta attaagacac aagtttaagc agatcttttg gttgtaagcg tttgaaaata
    30901 attaatttct tttttcatta ttttcagacg attttttatt gatttttttt tttcgcagaa
    30961 acagagaaat aaatatactt aacaacacaa aatgaattat ttatataatt ttattcaaca
    31021 aaatccttat ttgctgattc ttttttcttt tcttttggga aaagctttca tgccttccgt
    31081 tgttagaatt gccaagaaac ataattttgt ggtaaaaccc aacaaacgaa caactcataa
    31141 aggtttgata ccctatacgg gcggttttga tatttttatt tcttttatct ttacaatttt
    31201 aattttttgt ttccctttga tggagaaatt acaatatatc cccattggta ttttcattat
    31261 tttgatgacc ggttttattg atgacttgct gaatattaaa cccacatcca aattattggg
    31321 agaatttgtt gcaggagttt ttcttattgt gttttccgac atcagaataa ctaatttttt
    31381 tggtgttttc ggtattcatg aacttccttt gtcgattagt tatatttttt cttttttggt
    31441 ttttttactg attatcaatt ccattaattt aattgatggg gtagatggtt tggcttccgg
    31501 actcggaatt ttttacaatc tttttttcgg catttatttt catctgaccg gcaatttgtc
    31561 actggccatg attgcttata cgcttacggg ttcgttggcc gtatttttta tttacaacgt
    31621 attcagcaag aaaaacaaaa tttttatggg agataccggt tccctgtttt tgggatttat
    31681 gctgactttc tatctctttg aattctgtga aatgaatgct tatcagagtg ttcctcaaag
    31741 tatgtatatg gctgctgctc cggctgtagc tatttgtgtg ctgattgttc cgttatatga
    31801 cactttgcgg gttgttatta ctcgtttaaa gaaaggggtt tctccattta aggccgataa
    31861 aaatcatata catcatttat tgctgaaaat tggattttct caccgacagg ttacattcgt
    31921 tttattagct attaccgcct tttttacggc tttcgcactg ttagctcgtc aatggtcaaa
    31981 cttggtgttg gcgtttattg cctttttgtt ggctacgggt atgatttttt ggttatggcg
    32041 agttataaac agaaaaatgc aaagaaaata actgcttgca agtttattct tacattttct
    32101 attttttact ttttgaatat ttatgatttc agttgagcaa ctttgtgtcg aatttggcgg
    32161 aaccacactt tttgataaca ttacttttct gattcatccc aaagaaaaaa ttgcgttggt
    32221 agggaaaaat ggtgctggta aaactacttt gttgcgtata ttggcaggca aacagcaacc
    32281 cacacgcgga aacatatctt atcaaaaaga cattaccatt ggctatctac ctcaacacat
    32341 gattcatagt gagggaacaa ccctctggca ggagacggaa aaggcttttg ggaacattgt
    32401 gcaacttcga cagcaattgg aagacattca gcagcaattg gttgaacgta cagattatga
    32461 aagttcagca tatcattcct tgttggataa actctctcag ctgaccgaat atcttcaaat
    32521 ttccggaaat ggcaattatc aggccgaaat ggaaaaaact ttgttgggac ttggttttct
    32581 gcgttccgat ttttaccgac agtgttcaga attcagtgga ggttggcgca tgagaattga
    32641 acttgccaaa attttgcttt cccatcccga tgtgttatta ctcgatgaac ctaccaatca
    32701 tttggatatt gaatccattc aatggctcga aaattttttg ttcacttcgg gcagctctct
    32761 tttacttgtt tctcacgata aagcttttat agatgcggtt actacacgta ctttggaaat
    32821 ttcgttggga aaactgtatg attacgatgt gaattattca aaatatattc agttgcgcaa
    32881 agagcgacac gaacaacaat taagagctta cgaaaaccaa cagaaattaa ttcaggatac
    32941 ggaagatttt attgaacgtt tccgatacaa agctaccaaa gctgttcagg tacaatcgcg
    33001 tatcaagcaa ctcgaaaaat tgcagcgtat cgaagtggat ttggaggaca atgccaatat
    33061 gcacttgaaa tttccgccgg caatacattc cggaaaaatt cctctcgaaa tagaacacct
    33121 ttccaaagct tacagggaac atttggtgct ggacgatgta aatttgatga tgcatcgagg
    33181 agagaaagta gcatttgtcg gaaaaaacgg agaagggaaa agtacattgg taaagtgtat
    33241 catgaatgag atagaatatt cgggaacttt aaaattggga tacaatgtaa aaatcggcta
    33301 ttttgctcaa aatcaggctt ctttgctgga cgaaaatttg acggtttttg aaaccatcga
    33361 ccgcgtagcc gttggagaaa ttcgtacaaa aatccgggat attttgggcg cttttatgtt
    33421 tggtggcgaa aattccgaaa aaaaagtcaa ggtactttcc ggtggtgaac gcagcagatt
    33481 agccatgata aagcttttac tcgaacctgt gaattttttg attttggacg agccaacgaa
    33541 tcatctggat attccatcga aagaagttct taaagaagcc ataaaggcat ttgacggtac
    33601 ggcaattatt gtttcgcacg accgtgaatt tctggatggt ttggtaacaa aagtgtatga
    33661 gttcggcaat aaaaaagtga aggaacattt gggtggtatt tacgatttcc ttcaggcaaa
    33721 aaaaatggaa agtttacatg aacttgaaag aaatgtatct tctgaagtga aaaatgaagg
    33781 tgaagcaaaa ccggaaagtg aaaacaaatt gaattatgaa agtcgcaaag agctgaatcg
    33841 aaaaattaga aaattggaaa aagatgtgga agcaaccgaa ggcctgataa cagatttgga
    33901 caatgaaatt caacaaatga acacggattt gcaaattccc gaaaaggctt ctgatcccga
    33961 atttgtttca actcttcaga aaaagcgtgc cgagttggaa aggaaaatgt atgagtggga
    34021 aattttgagt gaagagttgg aaaaaattag aaaagaatgg caaggacaat aaatctcttt
    34081 tcgttccatt gtcggtaatt cgtttttaaa tttcaatcaa tgtattaaag ccgattttta
    34141 tgaaaacgtt gtatttaatt cccactccac ttggcaatac ttctttcgat agaatttttc
    34201 ctcctgtcaa tctggaaatt ataagaaatt tacgttattt tattgttgag gatattcgta
    34261 ctgctcgtag gtttttaaaa caggtcgacg tcgaaattga tattgataaa ttattttttt
    34321 atacgctgaa tgaacatact tctcccactg aagttgacgg atttttacag cctttacggg
    34381 aaggcgaaga catgggactt ctctccgaag ccggatgccc tgctgtagcc gatcccggcg
    34441 ccgatatggt agctatcgcc cagaaaaatg cttttcgcgt tgtgccttta gttgggcctt
    34501 cttcaatttt gctggcgcta atggcttccg gtttcaacgg gcagaatttt gcttttgtcg
    34561 gatatttgcc cgtatcgcct gcagaaagaa taaaagcgat tcgaaaactt gaatcaagag
    34621 cctattccga aaatcaaacg cagattttta ttgagacacc ctatcgtaat ttaaaacttt
    34681 ttgaacaaat tttgcaaaat tgccgtcctt ccactttgct ttgtattgca gccaatgtta
    34741 ctttgccgga tgaatccatc gttactcgaa gtgttgcaga gtggaaaaca atcactccgg
    34801 atattgataa gaaaccatgt attttcctga tttataaagg ttaaaagcac ttcaatctaa
    34861 ttttttttgg ttgataagaa attttcctcc tgacttcgac aatgcgtcac cctaaaaatt
    34921 tttttaaaat ttcatcagaa tataatttat tttttaatgg tctgatggaa cccacatgtc
    34981 tgaatattat attcattcgt gcttgtgtat gtaaaacatg tgggtttaat attctctaag
    35041 taaaatgatt attgctattg aaaattacaa aaaactattt cattaacaga gaactgtctc
    35101 cgtagctcag aaaacgaaaa tcgtgagaaa gggcataatc gtaaattttt cgccagttgc
    35161 catcaacaaa ggcagaaacc agcagtagca aagtactttt tggttggtga aagttggtaa
    35221 tcattccgtt ggtcagtcgg aatgtgtaac cgggttgaat aatcagttgc gtttgagcat
    35281 gaagtacgga gagatttctt ttctccattt cgttcaatat taattgcaat gatgcttccg
    35341 gagtcagaac acattcagtc tggtaaggca tccattgcgg gataaaaaag taaggatgct
    35401 tttcgtcggg aaattgttgc ttctcgataa gtaaaccaat gtggtacaaa ctttccagtg
    35461 ttcgtacgga agtggttccc acggcaatga tttttccgat attttttaac agcttttcta
    35521 ttgttttttt ttgaatttca atgacttccg tgtgcatttg gtgatcggaa atttgttttg
    35581 tttttaccgg ttgaaaagtt ccggcgccaa catgtaaagt caattcggca gtctctatat
    35641 tgcgggattt caaactttca aaaacttccg gggtaaaatg caatcctgcc gtaggagcag
    35701 ccaccgaacc tttgattttt gcataaaccg tttgataggt tatcaaatcg ctttcttccg
    35761 taggcctgtg caagtaggga ggaataggca attcgcctgc cttttccagg atttcggcaa
    35821 agtgaatatt ttcattgttc cagctaaaac gaacaaaatt tacattcccc tttctttcga
    35881 gcaattcggc ggagaactca aattgcgttc cgtttatgtc gagttctttg gacaaagtgc
    35941 cgtttttcca ttttttttga tttccgacaa aacatttcca aacacattct ttggttgccg
    36001 agagagataa tgcgtaatcg gccggctgag ctggctccag gcaaaagact tctatcaacg
    36061 cgccagtttc tttcctgaaa tgaatacggg cttggataac ttttgtattg ttgtaaacca
    36121 gcaatgcctt ctcgggcaaa aaagaagcaa tattgctaaa aacattttcg gtgattgttc
    36181 catctctgta aatcagcaac ttcgaagcat cgcgcttttt taacggatac ttggcaatgc
    36241 gttcttcggg taagacgtaa tcgaaatcat caatggaaaa gttcgttttt tcttttaatt
    36301 cgttcacggg agatacaaaa atattgttgt aattttgtgc aaagatacga aaaatcgaaa
    36361 aaatttccga tccgttcgta tctcactgca caaaagcagt ttggaagata aagatgtaag
    36421 aatgtttttt tgaaaaaccg aacaacatga taaaagtagg agataaagta cgttttctca
    36481 attctactgg tgggggaatt gttaccagaa tagatgaaag ggaaaaaatc gtgtacgttt
    36541 ccgatgctga cggatttgaa attccaatgc tggaacgtga atgtgtggtc attccggaag
    36601 tgaatgaaaa taccaatttt cccaaaaagg attttaaatc ggcttcagca gcttttatga
    36661 atgagaacga atcgccggcc gctaagtcca tcaacttacc accaaaacct gaaattatag
    36721 aggaagaaat ctttgaaacg cctgaaggag aaaacctgag agtttttctg gcttttgtgc
    36781 ctgaagatat caagcagttg cagaccacct cgaacgactt ttatttggtg aacgacagca
    36841 attattttct gttttacaac cttgcattgt ttgaaaacga atgctgcaaa agtgtggcca
    36901 acgggttgat tgaacccaat atgcaggaat ggatggcaga gattaaaaaa gaagagctga
    36961 atgcttggga aaagattcga gttcaaatca taccctttaa aaaacataaa acttatcggc
    37021 cgcaaccggt tattgatatc gatctcaaat tgaacctgat gaagttttac aagcttcaca
    37081 gtttcggtga aaatgagtat ttcgatgtgc ctgccatgat gttgaatcct ttggaagaaa
    37141 ttgaaacgca tcaattgcac gacatttcag cggaagaaat taaacaagcc atttcccaaa
    37201 aggaaaagcc cgaacggcga ccacgcatca gcaaacctgc aaaaaatccg gaaattgttg
    37261 aagtcgatct gcatatcaat cagcttgtgg atactacggt cggtatgaat aatgccgata
    37321 tgctggaact ccaactggaa aaatttgaat ccgttttgaa tgattacaaa aatcgaaaag
    37381 gacaaaaaat tgtcttcata cacggaaaag gagaaggagt gctgcgaaag gaaattgaaa
    37441 aattgcttcg taccaagtat aaaagttatt attttcaaga tgcttccttt caacaatatg
    37501 gctttggagc cacaatggtt actatccgtt aatcattcga tacttttgtt ttaggttatc
    37561 ggaatattta aaatttcgat ctgtccaatc attatcaggt ttcaactttc ttctcaaaac
    37621 cattgatttt tttaaataac agattttctg tctgttttca aaacaataaa gtttaacaga
    37681 cttataatca atttattaat aaaacactta actcctaact ctttactcct aactctttac
    37741 taaaaactcc taactccaaa ttcaaatctt catttcatta aaaaatattt acttaaacaa
    37801 atttagcatg atagacaaat tttttattaa acggtatttt ctttttgttt tcagtcttat
    37861 tttatcagtg tcgtttttct cgtgtggcga aaaggaagaa aatgagccgg atattaccaa
    37921 ttctatttat gttacgcagg tttttgaata tgtttatgca cccggccaac atgcccaact
    37981 ggcaaaacct gccgatatcg agcattttat tggtgagcct tcggatgaaa agggattttt
    38041 gtatttgggc ggttttggcg gatatgtaat tgccgggttc gaccacgata ttcaaaacaa
    38101 agatggatac gatttggaaa tatttgcttt gcccggagct tcgccggaac cggcaattgt
    38161 ttatgtaatg cccgacgaca atggtgatgg taagcccaac gatacctggt atgagttaaa
    38221 gggaaatcag tttgaaaatg aaaaaagaaa ttattgggtt cgttactaca agccagcgac
    38281 atccaatgac aacattcagt ggaaagacag tgaaggagag aacggagaat tgatttcggg
    38341 atttggagct cccaacactt cctcctggtg gtgggcaacc tgtatggaag acagtattac
    38401 ttttgaaggt tcacgtttgc cggatgctta taacaacaaa tcttctgaaa gtgcacaaag
    38461 ttggattgtt ccaactgatc gttttatatg gggttatgcc gaaaataatt ttggaaccga
    38521 ttatgatacc catcatcatt ccaataaact ggacatttcc aatgccgtcg attcttcagg
    38581 taaggctgtt tatctggaaa aaattcgctt tatcaaaatt caaacggctg tttttcagcg
    38641 tgccggctgg gtgaacgaag tttcggccga aattcgcgga gcagctgagt tgaaataatc
    38701 agacccttct tccataagct ttggaaacct gcataaaaat tcaaaattac tgtcggtctt
    38761 tctgttgtta caaaaatttt aaatatcttt gtgagtttaa ttttcctcaa cgtacaacaa
    38821 tcaatttatg gaaaatctcg agccttcagt tgtttggaaa tttttcgggg aaatcaccca
    38881 aattccccga ccttcaaaaa aagaagaaaa aataagggaa tatttgctgg atgttgccca
    38941 acaataccga cttgaggcaa aaacagacca ggcagggaat gtgctgattt gtaaaccggc
    39001 aacattcggt tacgaaaatc acaaaaaaat cattcttcag tcgcacatgg atatggtttg
    39061 tgaaaaagat ggcgatgtgc agcacaattt tgactccgac cccattcaaa cgtttattga
    39121 tggtgattgg ataaaggcgg aaggtacaac tttaggagcc gacaacggca taggcatggc
    39181 tatgatgctt gctgtgctta cttccgacaa catttcacat ccggcgctcg agtgcctttt
    39241 tactgttgat gaagaaacag gactgaccgg agcttcggct attgaagatc atttcctcag
    39301 cggacaaatt cttatcaatc tggattcgga agatgatggt gttattttta tcggttgtgc
    39361 cggaggtatc gatacgacaa taaattttcc ttttatagct gaaaaatctc cgaataatta
    39421 cttttccttc tcgctgaccg tgaaaggatt gaaaggtgga cattccggcg acgacattga
    39481 caaaagacac ggaaatgcca taaaaattct gaatcgtttt ctgtggaatg taaacaaaaa
    39541 acttgatttg agaatccata cttttcgggg aggaaatttg cacaatgcca ttccacgcga
    39601 agccgaagtc gtagcttccg taccttttga ggataaggaa aaacttcgtg tgttactcaa
    39661 cgaatttatt gcggaaatag agggtgaact caaagaagat gaaccggctt tgcaaatcct
    39721 tatttcttca gaagaaattc ccgatcaggt actcgacaag aaatcttccg ataagctttt
    39781 aaatgcactt tatgcctgtc cacatggagt aaaggctttt agcagagata tgcccaatct
    39841 ggtggaaact tccaccaatc tggcttctgt ccgaatgcag gaaaataatt cttttcttat
    39901 cataaccagc cagcgcagtt ccgttgaatc ggccaagttc gacattgccc atcaggttga
    39961 atctgtcttt acacttgcac aagctcaagc aacgcatggt gaaggatatc cgggctggaa
    40021 acccaatttg aattccgaaa tattgcagaa agccaaagtt tgttacaaaa atatcacgtg
    40081 tgaagaaccg aaagttcggg ctattcatgc cggactggaa tgcggacttt ttctgaaaaa
    40141 atatccttat ctcgacatga tttccattgg accgcaaatg tatggagttc attctcccaa
    40201 tgagcggtta agtatctctt ctacccggaa atgttggcaa tggttgctta aaattctcga
    40261 aaaaagctga aatgacaaat atctcgggaa acaactcgct cggagaaagt cggatacaac
    40321 ccaacaaact caaaattatc aatgatccgg tttttggttt tatcaacata ccggatgatt
    40381 ttttatatga aattattcag catccttatt ttcaaagatt aaaccgcatc aagcaattgg
    40441 gtgtttcgtt ctatgtttat ccgggagcgc aacatacccg tttgcaacat tcgttggggg
    40501 ccatgcactt aatgggtgaa gccattgaag agttgcgtca gaaaggtcat gtgatttccg
    40561 gcgaagaaga gcaggctgcc cgggcgtgca tgttgctgca cgacattgga cacggcccgt
    40621 tttctcatgc tttggaaaac agcttggtaa aaggaattca ccatgaagaa atttcaataa
    40681 tgctcatgca gcaattgaat aaagaaatga ataataattt ggctttggcg ctggaaatat
    40741 ttagtggcca ttatcctaaa aaatttcttc atcagttggt ttcgggacaa ctcgacatgg
    40801 atcgaatgga ttacttgagc agagacagct ttttttgcgg agtcagtgaa ggtatcattg
    40861 gttcggcgcg tattatcaaa atgttgaatg tttttcacgg agaattggtc gttgagttga
    40921 aaggaattta ttccattgaa aaatttctgg tggcacgtcg tttgatgtac tggcaggtgt
    40981 atttgcacaa aacgctggtt gctaccgaaa aaatgttgac aaatattctc aagcgtgcca
    41041 aggaacttgc ttccgttggt gaaacattat ttgcttctcc tgctttgcat tattttctct
    41101 atcatcatct ggacaagcag aattttgttc gttcaaccga agcattggat tattttgttc
    41161 agcttgatga ttcggatgtg atttgtgcca tcaaagtttg ggctcaccat cccgataaag
    41221 ttctttcttt tctttgcaat gcttttatca acagaaaatt gtttaaggta gaaatgctgc
    41281 aggagccttt ggacgaaaca aaaaaagagg aattattgaa aaaatacagc cggtattttg
    41341 atgtttcgca gcaggaagcc gaatatttca tgggagagga aatcgtcagc acagatacgt
    41401 atgaccctgt tgatgagaaa attaacattc tatacaaaga tggaacggtg aaagatattg
    41461 ccgacgcgtc cgatatgctt aatataaatg ttttgaccaa gaaagtttac aagaattatt
    41521 tctgttattg ccgaatttag ccgacagcaa gatatttttc tgtaaaataa aattgtagaa
    41581 taaaaataac aagtgttaca aagtgaataa ttaaaaattc tttcgtactt ttgtacccaa
    41641 aatttttaaa aactatggag tttacagctc agcaaattgc agatttttta ggtggccagg
    41701 tggaaggaga cccatcagta aaggtaagta atttttctaa aatagaggaa ggaaaaccag
    41761 gaaccataac atttttgtcc aatccaaaat acagtcatta tatttacgat tgtaatgcaa
    41821 gcattgtatt ggtaaacaag gatttcaagc ctgagaaaga aattccagcg acgcttattc
    41881 gtgtcaacaa tgcgtatgaa tgtttggcgc agcttttttc gctggtcgaa cagagcaaac
    41941 ccaaaaaaaa gggcatttct ccattggctt tcatagcttc tactgcccaa attggtgaag
    42001 atgtttacat tgctcccttt gtttatgtgg gtgaaaatgt aaaaatagga aaaaacacgc
    42061 aaattcattc tcatagcaat atcgaagaca attcttcggt aggcgaaaat accatattgt
    42121 tttcaggtgt acatgtttat cccgattgta gcattggcaa caattgcatt ctgcattccg
    42181 gtgtggtaat tggtgccgat ggttttggtt ttgcccagtc ggaagatggt acttacaaaa
    42241 aaattaccca aatagggaat gtcgtgttgg aagacgatat agaagttgga gccaacacca
    42301 ccattgatcg cgctacgttg ggaagcacca ttgttcacaa aggtgtgaaa attgataatc
    42361 tggtgcaaat agcccataac gacgaaattg gtgaaaacac agtaattgta gcgcaaacag
    42421 gcattgccgg ttcaaccaaa atagggaaaa attgtgtgat aggcggacag gtcggtatag
    42481 ttggacattt gcagattgct gacaataccc aaatcggggc acagtcgggt gttgccaatt
    42541 cggtaaaaac tcccggtcag attattatcg gttctcctgc tattcccatt cagaattttc
    42601 gccgttcttc cattgtattt aaaaatctct ccgatttaca aaaaatggtg atagaattac
    42661 aaaataaggt agaagagctg gaaaaaacta aaacttcaga aaaataattt tttctgaaat
    42721 ttttttttga ctgcctcaaa tgcataaaaa ttgttaatca aaaacaaatg acgagacaaa
    42781 gaacattaaa ggaatctttt acattagaag gaaaaggctt acacacaggt ttggatattg
    42841 tgattacctt tttgccggct ccagaaaatc atggaataaa gttaaaacgt attgatctgg
    42901 aagaacccgt tatcattgaa gcattggcag aaaatgttac ccacacggta cgtggaactg
    42961 ttctacaaaa aggcgacgtt caagtaagca cggtagaaca tgcgttggct gcgctttatg
    43021 cttttggtat cgacaattgc ctgatagaag tcaatgcacc tgaattcccc attttggatg
    43081 gaagtgcccg cttttatgca gaaaaaatta aagaggtcgg aattattgaa cagaaagcgg
    43141 aaaaagattt ttatgtggta agaaaaaaaa ttgaatattc acttcccgat tccaactcca
    43201 aaatcacttt gttgcccgat gatgttttca gtgtagatgt tcacatagga ttcgattctg
    43261 ccgtacttgc caatcagtat gcaagtttgg attcactcga taaatttgcc gatgaaattg
    43321 cttctgctcg tacttttgtg tttgttcacg aaattgaacc gctcttgagc atgaatctca
    43381 tcaaaggtgg cgatttgaaa aatgcacttg tcatttacga taaggaattg tcgcaggata
    43441 cgatggacag actcaccgac aaacttcatc agccacgtat tgatgcaacc aaacttggat
    43501 atatcaacgg tgaactgaat tatcccaatg agcctgctcg acataaatta ctggatgtta
    43561 tcggagacct ttccctgatt gggaaaccac tgaaagcaaa agttattgtg cagtatcccg
    43621 gtcataaaat aaataccaat ctggccaaaa tcattcgcaa ggaaataaaa aagcaggaag
    43681 tttcggctcc tgtgtacgac gattcggttc cgcccgtatt ggacattaat gctattagaa
    43741 aacttttgcc gcatcgctgg ccttttctgc tggtcgataa aattatcgat atcaacgaaa
    43801 gagtgattgt gggtgtgaaa aatgtatccg gcaacgaaac gttttttatg ggacattttc
    43861 ccaacgaacc tgttatgccc ggagttcttc tggtagaagc tatggcgcag actgccggaa
    43921 ttttggtgtt gaacaaagtg gaggaacccg aaaaatattc tacttatttt atgaaaatag
    43981 acaatgtaaa attccgtcag aaagttgtac cgggcgatac agttattttt cgattagaaa
    44041 tgatggatga aattcgtcat ggatgcaccg atatgaaagg ttacgctttt gtaggcggaa
    44101 aaattgttgc tgaagcccag tttatggcac aaatcatcaa aaacaaataa aactaaaaga
    44161 tttacaccca tacaatgaaa caaccgctgg catatattca tccggatgca aaaattgcac
    44221 cgtcggtagt cattgaacca tttgttacca tagataaaaa tgtggagata ggcgaaggaa
    44281 cgcgtatcgg tccgaatgtt accattttgg aaggagcccg aatcgggaaa aactgtacta
    44341 tttttcccgg tgccgtaatt gcagctattc cacaggattt gaaattcaaa ggtgaagata
    44401 ctttggccat tattggcgat aataccacca ttcgcgaatg tgttaccgta aacagaggca
    44461 cagcttcaaa aggaaaaacc caaattggcg acaattgcct gatcatggct tattgccaca
    44521 ttgcacatga ttgctttgtt ggcagcaatg tcattatgtc caatgccacg cagcttgccg
    44581 gcgaagtatt cattgatgat tacgcggtga tcagtggagg cgtcttggtg catcaattta
    44641 cccatattgg gtcgcatgta attattcagg gtggaacaaa agtgaataag gatgtaccac
    44701 cttacgtgaa agcagggcgt gagccgctgt catttgaagg aatcaattcg gtgggtttgc
    44761 ggcgacgcgg atttaccaat gaacaaattg gttttattca ggaaatttat cgctatatct
    44821 ttttgtcgaa aatgaatgtg agcaatgcca tagatcgtat cgaagccgaa ttgcccgcta
    44881 ccaaagaacg tgatgacatt cttcttttca taaaaaattc gcaaagaggg attatcaaaa
    44941 gttttctgga ataaacttga tttcatttct ctgtttttga gaaaaaattt tttatatcat
    45001 tcagaagcta tgaaaattaa cgaagaagaa actccaaaaa aaaatctgaa tttcattgaa
    45061 gctattgttg atcaggacgt taaggaaggg aaaaatggcg gacgtgttca gacaaggttt
    45121 cctcccgaac cgaacggtta tttgcatatc ggacatgcta aggctatttg tctgaatttc
    45181 gatattgcaa aaagatacgg aggaatttgt aatcttcgtt ttgatgatac caatccggta
    45241 aaagaagatg ttgaatacgt tgattccata atggaagaca ttcaatggtt gggttatcag
    45301 tggaaaaatg tttactacgc ttccgattat tttcagaaac tttgggattt agcagttcag
    45361 tttatcaaaa aagggttggc ttatgtagac gaacaaagtg ccgaaaccat agccaaacaa
    45421 aaaggtacac ctactgttcc cggaatagaa agtccttatc ggaatcgtcc gatagaagaa
    45481 aatcttgcgc tttttcaaaa aatgacgaat ggtgaaattc ccgaaggcgg catggtgttg
    45541 agggcaaaaa tcgatatggc ttctcccaat atgcactttc gcgatcccat catgtacagg
    45601 attattactt cccatccgca tcatcgtacg ggttggcaat ggaaagttta tccgatgtat
    45661 gattttgctc atggtcagtg cgattatttt gaaggagtta ctcattcttt gtgtacgctt
    45721 gagtttgaag ttcatcggcc actttacgac tggtttatcg atcagtatca ggataccgat
    45781 taccgaccgc gacaaataga attcaatcgt ctcaatctta cttataccgt catgagcaag
    45841 cgtaaacttt tgcaacttgt tcaggaaaag tttgtcagtg gctgggacga tccccgcatg
    45901 ccgacacttt gtggtttgcg tcgacgtggt tttacacctg aatccataca taattttatt
    45961 gataaaatag gctatacaaa atttgaagcg ctgaacgata ttggattgct tgaacatgcg
    46021 gcccgcgaaa gtctcaacaa aacggcaatg cgtgttaatg ccgtacttca tcccgtcaag
    46081 ctgattgtta ccaattatcc tgaaggaaaa gtagaaatga tgacaacgca aaataatcct
    46141 gaagaccctg aagctggaac cagagaagtt gtcttttcga gagagctttt tattgaaaga
    46201 gacgatttta tggaaaatcc tccaaaaaat tattttcgcc tgatgcccgg tcaggaagtt
    46261 cggctgaaaa gtgcttatat tgttaaatgt acgggttgca aaaaagatga aaatggtgtt
    46321 gttaccgaag tttattgcga atatgacccc atgacaaaaa gcggcatgcc tgaaagcaat
    46381 cgcaaggtga agggaacgct tcactgggtt tcggctgctc attgtgtgga agctgaagta
    46441 cgcctctacg accgcttatt taacgttgaa aacccttctg ccgacgaacg cgattttcgc
    46501 gaattactca atcccgattc cttacaaata cttaccggtt gcaaactgga aaattcactt
    46561 aaagatgcca agcctctcga acatttccag tttcaacgca ttggatattt tacagtcgat
    46621 ttggactcaa ctccggacaa gttgatattc aatcgtactg tctctctcaa ggatagctgg
    46681 agtaaagaac tcaataagta aaatcacctc aaatactggt caacaatgtt gcaataaata
    46741 tttgcagcat tgttgacttt tgtagtggtc tggattggaa ttcaggaatt ttttatgcaa
    46801 taaatttcta aattgagaat tctgtgttat attcgcagat gaattgcgaa taaagagaac
    46861 gaacttgatt gaaaaagttc attaacttat agtgctgtat ttcggatcta taaatgataa
    46921 agaaagcata ttcaatagcg caatgaatcg attccaatta aatttcttaa aaaatgttgg
    46981 agctcatccg agtcgattcg aaaatgtaca ccaaaaaaat ttggaagaat ggcgtgaata
    47041 ttatttcgat aatctcgggt caaaagaata tattattgaa cttgggaaaa agcttttatt
    47101 tagataactg aagtgatcca tgcagaagtt gcctaaataa ccgaacagga ttccatcgat
    47161 tacatgatgc aacaggtaat tgacagaatt tatgatggtt atgtaaccca aagttaaacg
    47221 atttatggac aacttcaaaa tatactacct gtaaaagtag aacttgcacc cgatgagcgg
    47281 gacagaagat ttaacatgga ctttttattc aaatagggga aaaattcatt cgactttaaa
    47341 ttgaaactgt ttctttaaga aatagaactc aacaaacaat taaaactatg gacttcagtt
    47401 taatacaaat tgccagtgcc tttatggtac tgtttgccat tattgatatt ttgggctcgg
    47461 tgcctattat tttggctatc aaaagtaaag gtgaaaatgt tcatgccgga aaaaccagct
    47521 tggtggcttt ggtcattctg gtggtttttt tatttgccgg tgactggatt cttcaacttt
    47581 tcaatgtcga tattgaatct tttgctgttg cgggttcttt cgtgattttt attttttcgc
    47641 tggaaatgat tctcgatgtg gaaattttca ggaacaaagg gccggaagga gcttcttcgg
    47701 tagtgccgat cgcttttcca ttgatagcgg ggccaggttc gtttaccaca ttactttcct
    47761 tacgcgcaga atatgccatc gaaaacatta taatagcgct cgtgctgaac atttgttttg
    47821 ttttcttcgt gttacggttc acatccaaaa tagaaaaatg gatggggaaa ggaggaattt
    47881 atattttaag gaaatttttc ggcattattt tgctcgccgt ttcagtaaaa ctatttacta
    47941 ccaacattag atttttgctg tagttggcgc gttttttcgg cattttttgg aataaaatat
    48001 attggcagaa attatcttgc tgctatcaat attcaaattt cccgaattcg ctttctatga
    48061 ttaattcttt tttgtcggat ggttcgcaat acccgataat ttttgcttcg acattgaatg
    48121 agcgggaaat gtcaatgact tcttctgcat aagcttcggg cagataaatt tccatgcgat
    48181 gtcccatatt gaaaacctta tacatttctt tccaatcggt ttgggattct tgctgtatga
    48241 tttgaaaaag cggcggcaag gggaaaaggt tatttttaat taccctgaga ttttcaataa
    48301 aatgtaaaat ttttgtctga cctcctcccg tacaatgaac cattccatga atttgcggac
    48361 gaagtttttc cagcattttt tttacaatcg gagcatacgt tcgggtaggt gaaagcacca
    48421 gttttccgac atcgataccg aggttttcaa ttttgtcggt taaatggtaa gaacctgaaa
    48481 aaaccagttc ggaaggaatt ttgtcatcgt agcattcggg atatttttcg gccaaataat
    48541 gtgcaaaaac gtcatgtcgg gcggatgtca aaccattgct gcccatgccg ccgttgtact
    48601 ccttttcgta actggcctga ccacttgagg aaagccccac aattacatcg ccgggacgaa
    48661 tattggcatt gtctattaca tcggctcttt tcattcggca agttacagtg ctgtccacaa
    48721 taatggttcg aaccaagtcg cccacatcgg cagtttctcc tcctgtgaaa tacagtttta
    48781 cgcccatttc gccaagttgt tgaatcagtt cgtccgttcc gttgatgata gccgaaacaa
    48841 cttcgccggg aatcaggttt ttgtttcttc caatggtgga ggaaaccaaa atgttatcca
    48901 ctgctcctat acacaacaaa tcgtcggtat tcatgattag cgcatcctga gcgatacctt
    48961 tccataccga caaatcgcct gtttctttcc agtaggcata agccaacgaa gattttgtgc
    49021 ctgctccgtc agcatgcatg atgttgcaat aatcgggatt gtttcccaga atatcgggaa
    49081 tgattttgca aaaagccttt ggaaaaattc ccttatccaa attccggatc gcattgtgga
    49141 catcttcttt tgatgctgaa actccgcgta aatcgtaacg ttggtttttc ataacagttt
    49201 taaatggatt tttaatattt tatttgcaaa tcatcggttt aattttcgca agaaataaga
    49261 acttcaactc atgtcaaaaa attatatttt cttgtttctt tgatgggtga aagtcaggat
    49321 acaggccaat aaaagaccaa tcaacaaatc gtttttggta aaaaagttga atatcacagc
    49381 ttcatccgga acgcgaccac tgaaaagtcg gagtaaaaca ataattacaa tatatactcc
    49441 aaatactcca agtccgtaaa gaagcgtttt caacagcgat ttcagtgttg gtttgttcat
    49501 aatttatgag tttaattttt caatcgatca aatcttccat aaaattttcg tccagttcca
    49561 ttttaagata tttccccgtt tcgctttcgg gatggcgagt tatttcggcc ggagttccgg
    49621 tgcaaataac ttttccgccg gcatttccgc cttcagggcc caagtcgatg atataatcgg
    49681 cacttttgat aacgtccatg ttgtgttcga ttataatcac cgtatttcct ttatcaacca
    49741 gccgattcaa aacatcgagc aaagcgcgta tatcttcaaa gtgcaagccg gtggtcggtt
    49801 cgtccaaaat ataaagtgtt tttcccgtat cgcgtttgga cagctcggtt gccagcttca
    49861 cgcgttgact ttctcctcct gaaagggtgg tgctggattg tccgagttta atatagccaa
    49921 gtcctacatc ctgaagtgtt tttattttat tgagaatgga tggctgattt tcaaaaaatt
    49981 ctaccgcctg attaatggtc atgtccaaca catcggcaat ggattttccc ttaaatcgga
    50041 cttccagtgt ttctctgttg tagcgtttgc cgttacaggt ttcacaaacc acgtaaacat
    50101 cgggaagaaa attcatttca atagttttgt aaccgtttcc ggagcatgtt tcgcaacgac
    50161 cgccggcagt gttgaatgaa aatcgaccgg gtttgtagcc gcgaatttta gcttcaggca
    50221 aagttgcaaa taaatttcga atatccgaaa aaacgtttgt ataggttgcc ggattggatc
    50281 tgggcgtgcg tccaatagga gattgatcaa cttccacgac tttgtcgata ttttccaatc
    50341 cttccatccg gtcgtaagga agcggatcct gtaacgaacg gtaaaaatgc tggctcaata
    50401 tgggttgaag ggtttccgtg attaatgaag attttccgct acctgaaact ccggtaatac
    50461 aaatcatgac gcccaaaggg aacgacacat cgatattttt caagttattt cctttggccc
    50521 cgataatggt cagaaatttc ccgtttcctt tgcgcacacg agcaggaatt tctatacgtt
    50581 tttctccatt cagataggca gaagtcaatg ttttggcttt caacatttct tccggtttac
    50641 cggcaaaaac tacttctcca cccaagcgac cggctcgtgg ccccatatcg actatataat
    50701 cggcagccaa catcatactt ttgtcgtgtt ccactacaat aaccgaattt ccggaatcgc
    50761 gcagttgttt caatgaattg atcagccgaa aattatcgcg ttgatgcagt ccgatgctgg
    50821 gttcgtccag aatatacaac acatttacca gttgtgaacc aatttgcgtt gcaagcctga
    50881 ttcgctgact ttctccacct gaaagcgatt cggagcttcg acccagagaa agatagtgta
    50941 accctacatc gaccatgaac tgcaaacggg tacgaatttc ctttaaaatt tcggtggcta
    51001 tggctttttg tttttcggta agataatttt caacgttccc taaccaattc aacaattcga
    51061 caatatccat tgcggcaagg tcggcaatgt tcttctcgtg aataaggaag tggagggatt
    51121 cttttcgcag tctggctcca ttgcattccg gacatttcac cgatttggaa tattgatttg
    51181 cccatttctg ttcggtggca gaagctctgt catcctgctg catttcgata tattttaaaa
    51241 taccgtcata attcaaggaa taattggtga tacccagact gtggttttta atcattaaag
    51301 gttcttccga gccattcatg atttcgtcga tagcctcttc gggaatatct ttgacaggag
    51361 ttttcagttg gcagccgtat ttctccaaaa ttgcttctat ttgccaaaaa atcacggtat
    51421 ttctgtattt tcccaatgga gctataccgc cgttgtaaat ggaaaccgat ttatcgggaa
    51481 taattttgtc catgtcaacc tgattgacaa atcccaatcc tttgcaagtg ggacaggcac
    51541 cgtgaggcga attgaaagag aaatgatgcg gatcggggtc atcgtaagaa attccgctgg
    51601 ttggacacat taattttttg ctgaaataac gtaccttgtc ggtctctttt tccaaaagca
    51661 tcacaattcc attgccttgc tgcatggctt tttgcatgga atccttgagt cggcgacgat
    51721 ctttttcggc gaccaccagt cggtcgataa caacttctat attgtggttt ttgtatcggt
    51781 caacgcgaag tccgggaacc aattccttca tttctccgtc gatacgtaca tgaagaaatc
    51841 ctttttttcg gatttgttca aacaattcct tataatgtcc cttgcgccca cgcaccaatg
    51901 gtgccagcaa gtaagtgcgc ttgccttcgt actgtttcaa aatcagttct acaatctgat
    51961 cttccgaata ttttaccatt ttttctcccg taacatagga aaaagcttca ccggcacggg
    52021 caaaaagtag tcggagaaag tcgtaaattt cggtagtggt gccaacggta gaacgtggat
    52081 ttttgttgac cgttttttgc tcaatcgaaa tgacaggact tagacctgta attttatcaa
    52141 catcaggacg ttgcagatta cctaaaaaat ttctggcata cgccgaaaaa gtttcgatat
    52201 agcgtcgctg tccttcagca taaatggtat cgaaagcgag ggatgatttt ccgcttccac
    52261 tcaatccggt gatgacggtg agggcatcgc ggggaatgga aacatcgata ttttttaagt
    52321 tgttgacacg tgctccaatg acttcaattt tttctgaagt atctgtattc gtgttattta
    52381 tattgttgtt gtttgacaaa gtcagcctcc tttaaattga acctgcaaag gtacaaaaaa
    52441 tgaaggtagt ttcaaatatt ggaataactc aaagcaatgc aaaaattgaa tattttgttt
    52501 tttcgaaaaa tattcttctc aataagccaa gagtaaaaaa ttttaataaa attataaaac
    52561 agaatgaatc atttcccaag aagttttcat cagttcggga atgcggtcgg gcagataagg
    52621 tgttacatca attggctgat gaataatcat ttctataacg cctggtttga ctttgaaact
    52681 cgtttttgat aatctttcaa agcttccttt gatggtaacc ggaacaatag ggagtgccag
    52741 gtcggcagca atttggaaag cgcctttttt gaattttctc atttgtccgt ttgttgaacg
    52801 cgtcccttca ggaaaaataa cgatggagtt tccttttttc aattgttttt cggctttttc
    52861 gatgctgagt tttgcggctt gtgcattcga acggtctacg aaaatatgtc cggccatttc
    52921 gctggccaat cctacaaaag gaacttttct cagttctttt ttcatgatcc atttgaaccg
    52981 tacgggaagc cagccataaa ttacaaaaat atcgaacatg ctttgatgat tggcagcaaa
    53041 aacataggat tttcctcttt caattttttc cagtcccttg actttcactt tgatgaagaa
    53101 taatccgcag gttgctttcg accaccatcg tgccggcaaa taagatatat tgctgttcgg
    53161 gaaaattgga gataaaatta tggtaaaaat agatgtcagc aaagttaaaa caattacaat
    53221 gggaacacat ataatccatt gatatacaga gaataaaaaa ttattgattt tcatggattt
    53281 gaaattattt ggaatagaaa aaaatcaatc aaagaatttt ataataaaac ttttttaaaa
    53341 tttttcgaga ttaaatcaat agcaagaaaa gaaaattttt cggtgcaaag atatatgttt
    53401 cccgtttatt tcaaaaagct tttagattaa acaaacaagt acggttgatt gtttatttga
    53461 acttgatttt acgcgcctga ctaacaaaca gtccaccaac aacagtagca attcctatcc
    53521 acttaagtat ggttaattgt tcgtttaata atagccagga gaaaatagcc gtaaaaaccg
    53581 gtatcaaatt ggtaaaaaca ttgctgcgag ctactccgat tcttctcact acccttgcaa
    53641 agagaacata agctattacc gaagcaaaaa tggacaacat aagcaatgct tcgatggaag
    53701 atagggtaaa atgcaaagtt gagaaatagg gataatcagc cagcaatgta gtgggaataa
    53761 aaaacatcaa tccgaaaagg ctttgataaa gaataatgtt cagcgtgtaa taatgaatgg
    53821 aaattttttg caaaactacg gcataacaaa cggccgaaaa aacggcaaaa aagagtaaaa
    53881 caatgccgag tggctggacg tggaaattgc cgcttggttc aaaaatgaca agaaaaacac
    53941 cggtaagcga caaaattatt cctgctacat tgaaccagcc gatgcgttct ttcaaaaaca
    54001 aaaaagccag tagcggcgaa aataccggaa tagttgaaat aataaccgat gccagtgtag
    54061 aagaaaccat tgtcaaaccg aaagtttcgc caatgtaata gaggtatggt tcaaacagag
    54121 ccagcagcaa aaaccatttc aaatcttgtt tctgaatttt ctgaaagcga cctgaaagta
    54181 tcgaaacgat gagcattagt acagaagcta tgattaatcg acaggttatc agcaacatgg
    54241 gtcgaaatga agtcaacgct tcttttgtcc agataaacga gaaagcccag ataagcatgg
    54301 cagcaacaat ggaagcatat aaactcaatg atttgaacct tgacatatat ttaagaatca
    54361 gcttgttatt ttaatttttg tttttatttt ttctatcgac aaagaatttt aaatcaaatt
    54421 tcaatcggaa atttttgtgt atatcaaaaa agaatattac ttttgccttc gctttttgaa
    54481 agcaatatca aattcttcct tagctcagtc ggttagagca tctgactgtt aatcagaggg
    54541 tccttggttc aagtccaaga gg
//
LOCUS       DAOSIB010000004        47739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_876_length_47739_cov_12.377569, whole genome shotgun sequence.
ACCESSION   DAOSIB010000004 DAOSIB010000000
VERSION     DAOSIB010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 47739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47739
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_876_length_47739_cov_12.377569"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1870)
                     /gene="gldG"
                     /locus_tag="PLD44_00865"
     CDS             complement(182..1870)
                     /gene="gldG"
                     /locus_tag="PLD44_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HOF97922.1"
                     /translation="MRNIIQHKQLFFWGVFLLLSLVLSFVHYRVDLTSEKRYSLSEPS
                     KKLMKKLDFPLKVKIYLDGSLNSGFLRLKKSTVEMLEELKVYSSKPIAIEFEDPSSVV
                     KPEEREKHYAELESRGLTPTNVYERDNDGKIIRKTIFPWLEMEYKGRKVLVNLLKNIP
                     GNSGDENLNISIIELEYEITDAVRRLVNTSVDKIAFLEGHDELSEAATYDISKSLSRY
                     FQIDRGRIGNDASILHPYKVVIVAKPQLPFSEKEKLIIDQYIMNGGKVLWLIDGVRLA
                     EENFSVTGTSPAIPLDLNLSDLLFRYGIRIKPVILQDVQCAQMPVNVAKTKEAPHFEP
                     LPWFFAPLLLTSYAHPVTRNLATVRSEFCSAIDLVGENQQVSAVLLLATSDNTHVIGT
                     PTTVDLRDMPSANDKNYFNTGYVPVGVLLEGNFESVFTNRMLPDSVVNISSIRPVSVN
                     TKQIFVADGDIIKNEISIVQGDSIYLPLGFDRLTGLQYGNRDFIENAVLYLADDEGWM
                     QLKNKTLKLRLLNRQVVYDQKLKWQLVNVLLPLVILAIFGFVFQLIRKRKFTRS"
     gene            complement(1967..2851)
                     /locus_tag="PLD44_00870"
     CDS             complement(1967..2851)
                     /locus_tag="PLD44_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOF97923.1"
                     /translation="MKKKIAFIINPMAGVRTKSFIPQHIHDELNRDLFDPTVAFTDYR
                     GHGSLLARDFAAKGFDSVVAVGGDGTINEVARGLVQTSVSLGILPIGSGNGLARHLEI
                     PMNMRKAIQMLNFSEEIFIDYGLVNQRPFFCTCGTGFDAYIAAEFAKGHRRGIVAYLE
                     KIVKGYFAYHSENYRIIGKNIDFTTKTFLMTIANASQWGNNVYIAPKASVQDGRLDVS
                     IMSNFPIIALPTLALQLFTKTISNDLFINTFRMSEITVFRPAPGPFHYDGEPYEEGKE
                     INIRVVPAGLKVLVKKRF"
     gene            complement(3294..4586)
                     /locus_tag="PLD44_00875"
     CDS             complement(3294..4586)
                     /locus_tag="PLD44_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOF97924.1"
                     /translation="MKTKKNFHSFPFKLWSLVLLSLPVCLPSQNNFQKNFQKNDSDFV
                     EFSGKVVDVKNGDVLPFATLSVEGSNITTVSNSEGEFSLKIPKEFQDKTLTVSYIGYK
                     NKILKISELKQENSRITLEPMIVSLPEINVISTDAENLVLSVLENRYKNYPEEELLMT
                     AFYRETIKKNRSYVSLSEAIVDILKQPYSSFRQDQAELYKSRKQSDYKKMDTLVFKLM
                     GGPYNSLYMDVMKNPDYLFTESILDNYEFSFDRTTYIDHQPIYIVNFKQRPFVNIPLY
                     YGKLYIDAESLALRSAIFHLDLTDKEKSASMLIRKKPLNAKVFLTNASYRMDYIEKDG
                     KWYYNYSRIELDFKIDWKRKLFNSNYYTVVEMAVTNWEKATEEKKLKNKLRPTVIIAD
                     EASGFSDPEFWGSYNVIEPEKPIESAIKKIQKQLEKRK"
     gene            complement(4876..5958)
                     /locus_tag="PLD44_00880"
     CDS             complement(4876..5958)
                     /locus_tag="PLD44_00880"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOF97925.1"
                     /translation="MKFSFCFIKKISVVVLTFFSLTLSAEIPEGYYRTAEGKTGAELK
                     TALHNIIRQHVILNYDTSTTVWWYTYFKTTDWHPDGYFWDMYSNNKRSTYNSSVMNRE
                     HCMPRSWWGTSSNYSTFDANGDLVNLSPSDAEANSAKSNYPLGIVGTASFDNGVVKVG
                     NNTYPGYTGLVFEPANQYKGDFARTYMYMVTCYEDYHSYWRGTGTSSMLDRNTYPVFK
                     QWAIKMLLEWSRNDPVSDKEINRNDGVYRIQQNRNPFIDHPELAEYIWGTSKGEVWNG
                     TAGNNDLYVNLSVYPNPASTSVLVNLNKPESSYYRIYDLSGISVKIGNFSSDGKLDVS
                     ELQIGSYLLVVYYGNQRKTIKLMIAH"
     gene            complement(5973..6593)
                     /locus_tag="PLD44_00885"
     CDS             complement(5973..6593)
                     /locus_tag="PLD44_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOF97926.1"
                     /translation="MYRFPSGATGSSLIVEAVKNGNYVRIDSIGSVNTSKNYPMYTFQ
                     ESDDIHSFRFIYKKVSGNLALDDVEATYKQQDTVFVLKNFQVSGSQFKVEGLNPNSVY
                     FYRVRSTIHPYTSDFSETIQVKTGFPSALENIPASQYKIYISSGDIKLSNLQGNERVQ
                     LFNIAGVCLFDQHSSGSEMSIRIPHRGIFIVRISNKDFTFARKLIK"
     gene            complement(6639..8807)
                     /locus_tag="PLD44_00890"
     CDS             complement(6639..8807)
                     /locus_tag="PLD44_00890"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOF97927.1"
                     /translation="MKKFFSTLFLILIVWLNCQIAFSEVPAGYYYLAHNKKKAELKSA
                     LSQIASPLRVLNYGSGKGYTWEGFYYADQNPDGSVKDMYSSEIRYFKDFSSVAGMHIE
                     HSLPKSWWGGHENNAYKDLFHLYPADGVTNSTKNNLPLGEVTGTPNFDNGVSKIGKNG
                     FGGIYTDNCFEPADEYKGDFARSYLYVSTVYENFATLWNSPMMNNNTYPVWKKWAIDL
                     LLKWNREDPVSQKERDRIEVVYNIQGNRNPFIDYPDLAEYIWGADTTKFFPFPEETEP
                     FLIYPKRGDQMDFGVILQTDSIQQHLTVSGLNLQNNLNIRLKGKSKSFKISKASLTKE
                     EVINGTEVIITFRPVVSGWNRDTLLLTSDGWKDSLRIPLQGLASTDFLALEPQNITPT
                     GGTLRWIFDPDADNYKLNLYQGDQQAGDLIISAYVEGSSWNKALELFNGTGKNIDLSN
                     YSLQKQSNGDGNFGATLKLNGMLENGKTYVLVHKSCSNNDLKAKANLLTDSLLSFNGN
                     DAVALLRNGVFIDVVGIANAGADVYWGQDLTLKRKATVTHPSSQFRDIEWNFYGIDDF
                     QMLGNHQMNLQPSSNYLLNDFETGKTDHFTISQLLPENTYTYSVTAIYPDEEKKALNT
                     MQIHTSKLEVPVIMNATDVRANSFVAGWEDCLYTSDYLLDVFQIQSGKDTTETESFDN
                     IGSNGKPLPEGWSGTASGNYTTAASSGKNPNALAFKNDGE"
     gene            complement(8893..9948)
                     /locus_tag="PLD44_00895"
     CDS             complement(8893..9948)
                     /locus_tag="PLD44_00895"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOF97928.1"
                     /translation="MKKNFLPLLLLFLNLTVVFSQPKQGYYNQAVGKSGAELKTTLYS
                     IISSGYVSKSYDFLYTIYETSDVTPEGLIWDMYSDCTWVPGQKKCGNYSKVCDCYNRE
                     HSIPQSWFNSQSPMVSDAFHVYPTDGKVNGQRSNFPYGECSGGITLSGGRGRLGNSTF
                     TGYSGTVFEPDDEYKGDFARTYFYFATRYQNIMTTIDGDSFNKTTYPALSDWAKALFL
                     KWHRQDPVSKKETDRNNAVEVYQKNRNPFIDHPELAEYIWGTKVGEPWSLSSGIENLK
                     IDFSISPVPAQDFLNIKTDENELMCTIFNIQGQELMKTLIDGNSSISVSQLNNGMYLL
                     QLEKDGKKTIQKFMVSR"
     gene            10366..10836
                     /locus_tag="PLD44_00900"
     CDS             10366..10836
                     /locus_tag="PLD44_00900"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOF97929.1"
                     /translation="MEFQEKMMRKAIALSLENIKKGGGPFGAVIVKDGKIIATGVNRV
                     TSNNDPTAHAEITAIRKAAGKLKNFDLSGCEIYTSCEPCPMCLGAIYWAHLDKMYFAN
                     TKADAQNAGFDDSFIYEEINLKPEDRKLKSQQLLREEALAAFETWKNTTNKTEY"
     gene            10925..14251
                     /gene="mfd"
                     /locus_tag="PLD44_00905"
     CDS             10925..14251
                     /gene="mfd"
                     /locus_tag="PLD44_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOF97930.1"
                     /translation="MLRVKEWANSLEQNLKISGLSGSSSALVFSSLFKAVPQHHIIIM
                     NDADEAGYLYNDLKLLLSENEIYFFPSSFKRAIKLSQLDAANDILRTEVLNRLANNSQ
                     PCIVVTYPDAIMQKIVSLQSLRQKTLKLHIGENIGIDFINELLFDFGFQRVDFVYEPG
                     QFSIRGGIVDIFSFANELPYRCDFFGDEIESIRVFDIETQLSKEEKQEIEIVPDLQRE
                     NKLPHVSFFEFIPKNTLLSFTHPNFIHDRINQLYDEFLVKANSEGHETPHLHKTMITG
                     DQWLELIEPFRKINWGTDRTFFKTSSQISFNTSPQPVFHKNFDLISDHLKKKIEEGYR
                     IYIMSDSVKQTDRIAAIFADRGDNIHFQPIKSTLHEGFVDHDLSLLCYTDHQIFDRFH
                     KYQLSTDKTRQGKVVMTLKELNQLKIGDYMVHVDHGIGVFGGLVRTEVNGKMQEMVKL
                     IYKDDDILFVSIHSLHRISKYKGKEGESPHINKLGSGAWERLKERTKSKVKDIARELI
                     KLYAQRKAEEGFRFSPDTYLQQELEASFIYEDTPDQTKATVEIKKDMESTLPMDRLIC
                     GDVGFGKTEVAIRAAFKAVADNKQVAVLVPTTVLAVQHYNTFKDRLHDFPCNVEYLTR
                     ARSAKETKEILERLAKGEIDIIIGTHKLIGKSVKFKDLGLLIIDEEQKFGVAVKEKLK
                     QMKVNVDTLTMTATPIPRTLQFSLMGARDLSIISTPPPNRYPVQTEIHRFDEDVIREA
                     IQLEMNRNGQVFFVHNRIQNIYSIQAMIKRLVPGCRVAVGHGQMPPDELEEVIVDFIN
                     YDYDVLVATTIIESGIDIPNVNTIIINNAHTFGLSDLHQLRGRVGRSNRKAFCYLLTP
                     EFQLLTTEARHRLEAIETFADLGSGFNIAMQDLDIRGAGNLLGAEQSGFIADLGYETY
                     QKVLNEAVQELKDEEFADLYAEVEEKTTTGGTYVTDCQIDSDLELLFPPDYVENVSER
                     INLYRQLDSIENENQLAAFEKELEDRFGKIPPQGKSLILMVRLRWMAINLGIEKMVLK
                     NQQMTTYLVSNQKSHYYQSDTFGEILRYAASHPHQCQLREKNGRRSIIFLKVTSVEKA
                     WEILSEIYRNLETAKLQKAV"
     gene            14696..14836
                     /locus_tag="PLD44_00910"
     CDS             14696..14836
                     /locus_tag="PLD44_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97931.1"
                     /translation="MKLFEQLEPHRNDYFGSFSVGTLELVFNDWYQRKKKVKTLHRFK
                     VE"
     gene            15094..15951
                     /locus_tag="PLD44_00915"
     CDS             15094..15951
                     /locus_tag="PLD44_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF97932.1"
                     /translation="MIQIKHVEFSYSRNAQLFTGLELSLSPGHIHGILGKNGEGKSTL
                     LKMISGMIFPQKGEIEVLGYEPRKRQPEMLREIFFLPEELPPITLSIENYEKVYAPFY
                     PHFNHEQFQGYLQEFEINSGKSQINKLSYGQKKKVFMAFGLATNTKIVLLDEPTNGLD
                     IPSKAQFRKMTASALEEERIMLISTHQVRDLDSLIDSIIIMNSHQIVFNELCENITEK
                     LLFSYGKEKSEGEEVLYSEENLRGFDQVSINKTGEESKLDIELLFNTVLKNHQRIREI
                     FSNQNNKRF"
     gene            15964..16731
                     /locus_tag="PLD44_00920"
     CDS             15964..16731
                     /locus_tag="PLD44_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97933.1"
                     /translation="MNQKFSLKRIGLLLRRYFIENQNYELYLWSILTIVFMIFRNGNS
                     ASAILIVTGALYAVRFFKVFNFAPNGIQYLLIPATQLEKTISVFLLNTVYFILMFTVV
                     FSVGNVIGNLLEQLIFSTSDTVALQFFQSSKGFEFNIQGLKNIWISFGVFAAIQAIFT
                     LGSLYFKRNAILKTLLSLFVVALALGILEYFIFKITFGSVTETLKALNAGDFVWDENI
                     FPKALTETWKIFWSISGYLFIPFCWIVSYFRLTEKEI"
     gene            16733..17107
                     /locus_tag="PLD44_00925"
     CDS             16733..17107
                     /locus_tag="PLD44_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOF97934.1"
                     /translation="MEFNDAQAIYLQIADYISEHILLGKWKEGERLLSVRELGTELEV
                     NPNTVMRAYDFLQSNEIIYNKRGIGYFVADDAKEKILNYRRKRFLEKELPILFKNIHL
                     LNIDVEEIKKRYNDYLQKQQAL"
     gene            complement(17508..18161)
                     /gene="nth"
                     /locus_tag="PLD44_00930"
     CDS             complement(17508..18161)
                     /gene="nth"
                     /locus_tag="PLD44_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOF97935.1"
                     /translation="MTTEERYLHILDWFMENMSNAQTELNYSNPFELLVAVILSAQCT
                     DKRVNMVTPQLFSDFPTPEAMASTTPEKLLEYIRSVSYPNSKAKHLVEMSRKLVADFE
                     GKVPDNVDKLQTLPGVGRKTANIIVSVIYNQPAMPVDTHVFRVSERLGLTTDSKNPLQ
                     TERELVRHIPPQLLPIAHHWLILHGRYVCHARKPKCNVCGLKSWCRYYFNHSLEQDI"
     gene            complement(18307..18762)
                     /locus_tag="PLD44_00935"
     CDS             complement(18307..18762)
                     /locus_tag="PLD44_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOF97936.1"
                     /translation="MEQLFEKVSADIKTAMLAHDKVTLEALRGIKKEFLEAKTAKGSD
                     GTLSDEAAIKILQKMVKQRKETAQIYKEQNRPDLEQNELAEIAVIEKYLPAQMSESEL
                     EATLREIIAQTGATGPQDLGKVMGVATRQLVGKADGKAISEMVKKLLSS"
     gene            complement(18814..20094)
                     /gene="ftsZ"
                     /locus_tag="PLD44_00940"
     CDS             complement(18814..20094)
                     /gene="ftsZ"
                     /locus_tag="PLD44_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOF97937.1"
                     /translation="MINIEELVEMLPLELPEIENSIIKVIGVGGGGGNAVNHMYRQGI
                     TDVSFVVCNTDNQALIKSPVPLKIQLGANTTSGLGAGGKPEVARQAAEESIDKIQNIL
                     QDNTKMVFITAGMGGGTGTGASPVIAKAAHDMGILTVGIVTIPFAFEGNLKIRQALEG
                     VAALSEHVDAILVINNEKLKEIYPDLELSNAFAKADDVLTNAAKGIAEIITIPGYINT
                     DFADVYSIMKDGKVAIMNTGYASGENRITKAIENALNSPLLNTSDVSGASKVLLSLYC
                     SSTHQIKMEEVQQIHDFMRKVGENVQVIWGATFDDELEDRVKITLIATGYDVSDIPGM
                     PASVIKNKKRLETTPDKIEVFSELKPKENQPESKEKIIENAVNSYYGKSIPDEKPIEE
                     PELDLPVVNLEDLENDQTLKYIEKFPSWKRKKGK"
     gene            complement(20124..21386)
                     /gene="ftsA"
                     /locus_tag="PLD44_00945"
     CDS             complement(20124..21386)
                     /gene="ftsA"
                     /locus_tag="PLD44_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOF97938.1"
                     /translation="MNQNTYIALDLGSSRITAMAAEVLESGAVKVLATESKAADDVYH
                     GIIEHTSGAAFKISELLKLLQNSAKIRDIQQVCVALNAKSMKLSKSSTTRFIGQGKIV
                     TDALLDEMKEECERKNSDENNVVFDVIPVSFEIDGKITTDPVGQSGNQITGNYHVVKG
                     NARILQQLKSCFVRNQIQIEYAPLSVEALSTVLTEEEERQNGCALIDFGASTTTLAVY
                     KNDVLQDLLVVPLGSKNITYDIAELGIEESNAERLKCLTGVAMEKFVEKPVYVQVAPS
                     SGTDPVRISTLFLAQIINARLDEMTEPVFKVLEVLSFPLESGIIITGGGAKLKFLKEF
                     VEEKSGLKVRWGNHSDWLEDQAPDYLADSSYSQLIGTILLANENRRLHPLKSENTTKK
                     GQKNKKKITERITDGFLRFFDDETTWNK"
     gene            complement(21431..22180)
                     /locus_tag="PLD44_00950"
     CDS             complement(21431..22180)
                     /locus_tag="PLD44_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HOF97939.1"
                     /translation="MKPVFKYILITFGILLVTGYLAWALLTFPDPKANEVCNRFHIVF
                     LDQSGVKLISEKEVAEMIDIKGLNPMGKTLKHVRTEEIEELLTKHPFIKQAECFKTPS
                     GGVYLFLQQRHPKFRLAGNENFYVDDEKNIIPVSYNDAARLPVVSGLVTKSMAKGELF
                     GFVSYLEENPFWNAQIEQIYIRPDRKIELVPRVGTGIILLGNLKDYEKKLHKLKILYE
                     KGFNEFGWNYYKTIDLQYENQIVCTKTDNID"
     gene            complement(22296..23678)
                     /gene="murC"
                     /locus_tag="PLD44_00955"
     CDS             complement(22296..23678)
                     /gene="murC"
                     /locus_tag="PLD44_00955"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOF97940.1"
                     /translation="MDFTKYYFLGIGGMGMSALARYFNSKGFQTAGYDRVQSKVTADL
                     QAEGIQINFDPNVDQIPISFRYPENTLVVVTPAIPDDLPQLVYFRENGFTIKKRAELL
                     GDITRKASTICIAGTHGKTTTSTLTAHLLYQSHISCNAFLGGIANNYNSNLLLSKDSN
                     LFVIEADEYDRSFHQLSPYMAAITSADPDHLDIYQTPEAYRESFEHFTSLISPGGVLL
                     MKKGVNIQPRLQKGVKQYTYSMNDGGDFYAENIHLVNHEFHFDFVTPSERISDIRLKI
                     PIRINIENSVAAIALAWLNGVTKEEIRTAIASYSGIYRRFNLVFRSEQTIFMDDYAHH
                     PKELKASILSIKELFPEKKITGIFQPHLYSRTRDFASDFAEALSLLDELILLDIYPAR
                     EEPIEGISSEMIFRNVKLDNKTLCSRENLLSLLKDKHPEVLVTFGAGDIDKLVPLIKK
                     QLMEQTNGNA"
     gene            complement(23725..24831)
                     /gene="murG"
                     /locus_tag="PLD44_00960"
     CDS             complement(23725..24831)
                     /gene="murG"
                     /locus_tag="PLD44_00960"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOF97941.1"
                     /translation="MPYRFIISGGGTGGHIFPAISIANALKKQLPDAEILFVGALGRM
                     EMERVPAAGYPIKGLPVSGFDRKNPFRNIKVLLNLRRSLQMARQIIRDFKPDAVIGVG
                     GYASAPTLKAASDLGIPTLIQEQNSYAGITNKLLAKKAARICVAYEGMERFFPKEKLV
                     LTGNPVRQELLSIPEKSKEAFEFFGFRPEKPTLLVVGGSLGARTINRSIISHLQELSQ
                     ADIQIIWQTGKFYIEEARKAAEPFSSPQLIVTDFVSRMDLAYSVADLVISRAGAGSIS
                     ELCLLGKPAILVPSPNVAEDHQTKNALALVQKEAAVMIKDEEAEKMLIPKALELIKDA
                     DSLNKLGQNIRKLAQKDSAERIAAEVLQLISKKN"
     gene            complement(24914..26146)
                     /locus_tag="PLD44_00965"
     CDS             complement(24914..26146)
                     /locus_tag="PLD44_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOF97942.1"
                     /translation="MENFLKKYLKGNATIWIVFILLCLVSILEMYSASSTLAFKSSNT
                     AMPLLRHAGFLLFGVILAFLVHLIPYKYIRLLAYLGLFLSFILLILVYYKGQSANDAT
                     RWFEIGGLQFQPSELAKLSVIIVVADFMSRIKNEAADEKKYFRYILIVLSAICPLILF
                     ENFSTAMLLFGVVFLMMFIGGISIKKLGLLLLSITLFVLGGYAMIKTVPEESMPKIFD
                     RAYTWSARIDRYTEEKKQEENKYVITDENRQVQHGRIAIARGGMLGVMPGNSVERDYL
                     PQAYSDFIYAIIVEEMGLVGGVIIILFYLVLLYRAGRIAVKSETVFPALLVIGLSLMI
                     VLQAFVSMAVATSLGPVTGQPLPLISRGGTSIIITCIYFGIILGVTRQLKGEQHVEEE
                     IPEEQERNFPVVDLNELS"
     gene            complement(26183..27517)
                     /gene="murD"
                     /locus_tag="PLD44_00970"
     CDS             complement(26183..27517)
                     /gene="murD"
                     /locus_tag="PLD44_00970"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOF97943.1"
                     /translation="MKRIVVLGAGESGAGAAVLAKQQGFDVFVSDLSEIKENYKELLN
                     QYQIEWEEKQHTEALILNADEVIKSPGIPDKAPIIKNLKEKNIPIISEIEFAGRFTNA
                     KMICITGSNGKTTTTMLTYHILKNAGYNVGLAGNVGQSLAYQVATSSRDYYVVEISSF
                     QLDGMYDFKADVAILLNITPDHLDRYEYNFQNYVDSKFRIIRNQTSEDAFIFWDDDPV
                     IQSEITKRSILAAKYPFSLKKKEHCKAYIENNNLIIETSNNPLIMPVKELSLKGIHNT
                     YNMMAAGLAASAVNVKKEFIRQSLQDFQGVEHRLEYVATVKNVTFINDSKATNVNSCW
                     YALESMKTPVILILGGTDKGNDYSEIESLVKEKVKALVFLGVDNTPLHRFFDGKIDKI
                     VDVRSMEDAVKAAYAMAEEGDTVLLSPCCASFDLFQNYEDRGRQFKTCVRNL"
     gene            complement(27557..28819)
                     /gene="mraY"
                     /locus_tag="PLD44_00975"
     CDS             complement(27557..28819)
                     /gene="mraY"
                     /locus_tag="PLD44_00975"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOF97944.1"
                     /translation="MFYHLFRYLDKFYDIPGAGMFNYISFKTGLSFIFALLLSTLFGR
                     RIIDFLQKKQIGETIRDLGLEGQLSKKGTPTMGGIIIINAILIPTLLFADLTNIYIIL
                     MLITTVWMGAIGFVDDYIKVFLKNKEGLSGKAKIVGQVGLGLIVGLVMYFSPDVVMRE
                     NTEIRGELRVEDVVYSKKDIKATRTTIPFVKNNDLDYSNAFKNAGKNAQKYGWILFVL
                     VAIFIVTAVSNGANLTDGLDGLSAGSSAIMGGALGVLAYLSGNINYSGYLNIMYIPGT
                     GELLVFAGAFLGATIGFLWYNSFPAQIFMGDTGSLTLGGIIAVFAIAIRKELLLPILC
                     GVFLAESLSVICQVSYFKYTKKRYGVGKRIFKMTPLHHHFQKPGNAGIEALIQRPLQA
                     VPEPKIVVRFWIVSMILAALTIITLKIR"
     gene            complement(28914..30359)
                     /locus_tag="PLD44_00980"
     CDS             complement(28914..30359)
                     /locus_tag="PLD44_00980"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOF97945.1"
                     /translation="MILKVALKKIFPTEIIGNTEMEISGVQSDSRKVDKGNIFVATRG
                     TNVDGHNYISPAIEKGASAIVCETFPETIQQGIVYIRVENSLEALGLLACAWYGNPSE
                     KLTLVGVTGTNGKTTIATLLYQMFRQLGYKAGLLSTVRNYVVDEPVEATHTTPDPLEL
                     NHLLAQMVEAGCQYAFMEVSSHAIDQRRIAGLLFKGGIFTNITRDHLDYHLTFENYLK
                     AKKKFFDDLPENAFALTNLDDKNGLVMLQNTKAKKYTYSLRSMADFKTRILEQDFEGM
                     LLEMNGKQVHVSFVGRFNASNLTAVFGAAVLMGEEETEVLRILSLLHPVSGRLETLHA
                     PSGYTVIVDYAHTPDALNNVISTIHKVLQNKGRLITVVGCGGNRDKGKRPLMAKEAVT
                     GSWKTILTSDNPRFEDPMDILNDMIAGLTDEERKTSLVIPDRRQAIHTACSLAQPGDV
                     VLIAGKGHEDYQIIKGEKHHFDDREVVKECF"
     gene            complement(30377..32518)
                     /locus_tag="PLD44_00985"
     CDS             complement(30377..32518)
                     /locus_tag="PLD44_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOF97946.1"
                     /translation="MADQHKNIVFRFGIVYVFIVVAFLLVIYKIITIQFFERENWLAL
                     AAKNVKTNIEVKANRGNIFSSDGRLMASSIPTYYIYMDLRVPALQENNGQLFHENIDS
                     LSLCLSKFFKDKSQAEYKKDITEAYNKKLGAFRIYPKKISYSQLKELKTFPLFRLGKN
                     KSGLITKEQFRRVKPFGSLASRTIGDIYADETKGGKNGLELKFDSLLRGTPGIAIRQK
                     VANTWQETVQVEPIDGIDVITTIDVDIQDIAEKALLDSLRSFDAQEGYVIVMEVQSGE
                     VKAIVNMQKNENGTYSENRNGAVSDMVEPGSTFKVVSLMAALDDGKVKVTDSIDTGNG
                     IFQFANAFMKDHNYNRGGFHKITIAEAIHASSNIGISRMIVNAYGNNPAAYVDKLYRM
                     KVNEPMNLEIPGTAAPNIRHPLDKSKYWSKTTLPWMSIGYETQIPPIYTLAFYNSIAN
                     DGKLIRPYFVKALSKNGQILKTFQTEIINEQICKPSVLQTIRETLLGVVEGRMGTAKN
                     VRSKYVRIAGKTGTAQISKGALGYRSGGKSHQVSFCGYFPAEHPLYTCIVVIREPRIG
                     YPSGGKMSGSVFKDIAERIMFLRSTLKPEHFVPDTMRNVPKVPYALWGSFSDLEKVTS
                     EMRLPISGTAGSFVNVLPDEKNNGLEISKKTVYRQLMPDLKGMGAKDAVFVAEKLGLK
                     VMISGRGKLVSQSISPGTKITKGQILSLHFQ"
     gene            complement(32527..32907)
                     /locus_tag="PLD44_00990"
     CDS             complement(32527..32907)
                     /locus_tag="PLD44_00990"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOF97947.1"
                     /translation="MSWFSKIIENIKGSEDFSELKSSSFRDILNGNIFTKRFFIKQIG
                     LLIMIVVLFIFYIGNRYEYESELTYQNKLKKEVQDKKYESLSISAELMQVSRQSNVEK
                     MLKERGIELHEPVSPLVVLDDSIQ"
     gene            complement(32955..33875)
                     /gene="rsmH"
                     /locus_tag="PLD44_00995"
     CDS             complement(32955..33875)
                     /gene="rsmH"
                     /locus_tag="PLD44_00995"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOF97948.1"
                     /translation="MSEKEYHIPVMLKETLQGLVLNPDGIYVDVTFGGGGHSRGILDL
                     LSKKGKLIAFDQDQDACQNIFEDDRFLFVQSNFCYLKNFLRYHGISLVDGILADLGVS
                     SHHFDESERGFSFRLDGELDMRMNRKTELTAATVLNTYTEEQLADVFYHYGELSNARK
                     IAATIVKARDREPFQKILPFVEMMKRFFPRQKEKKGLACVFQALRIEVNHELEALQEF
                     LQQTPDVLKPGGRLAVLTYHSLEDRMVKNFMKTGNFEGILNKDFFGNIIAPFKPISGK
                     AITPQLSEIEKNPRARSAKLRIAELIDKTN"
     gene            complement(33890..34339)
                     /locus_tag="PLD44_01000"
     CDS             complement(33890..34339)
                     /locus_tag="PLD44_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOF97949.1"
                     /translation="MSTFIGKYEAKADVKGRIFIPSAYRKILGVDDREQIIMRKDAGN
                     ECIVFYPESIWNQKVENLKSKLDEWNPSDQLLLMQFVADAEWLDIDAQGRVLISKKHL
                     QTIGVENAEVLFVGMIDRFALWSKSRYEQMQLSAENFAALLKEKMSK"
     gene            34679..35509
                     /locus_tag="PLD44_01005"
     CDS             34679..35509
                     /locus_tag="PLD44_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOF97950.1"
                     /translation="MNSKPDFLIDIEKVVKEKAPNTFIPSFLVSYLRKIVHEKEINEL
                     LTTYSHQKNVDFLESAVKYFQVNVSIKNRENLPHDDKLYIFAGNHPLGGMDAIMTGSL
                     LGREYDGKIRFMANDMLMFIEPLKDLFIPVNKVGSQGKNHVRELNDFFSSDCHLITYP
                     AGKCSRKSNGKIQDEAWNKNFVVKAIQHQRDIVPIYFDGKNSNFFYNLANLRKFFHIK
                     FNIEMLYLSDEMFKQKGKSFSLVIGEPIRWQTFDRSKTYGKWAQWVKEKVYALSEKTF
                     "
     gene            complement(35597..39895)
                     /gene="rpoC"
                     /locus_tag="PLD44_01010"
     CDS             complement(35597..39895)
                     /gene="rpoC"
                     /locus_tag="PLD44_01010"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOF97951.1"
                     /translation="MAFKTDNKIKSNFSKISIGLASPEEILKLSHGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIQ
                     LVVPVAHIWYFRTLPSKIGYLLGMSSKKLESIIYYEKYVIIQGGILENGDNIATGELL
                     TEEEYLDILDSLPKDNQQLEDTDPNKFIAKMGAEAIYDLLSRIDLDALSYELRHKANT
                     DTSQQRKAEALKRLQVVEAFRASKNRNRPEWMIVKIVPVIPPELRPLVPLDGGRFATS
                     DLNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLFDNSRKASAVKTEAN
                     RPLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKMHECGLPKDMAAELYKP
                     FIIRKLIERGIVKTVKSAKKIIDRKDPVIWDILEYVMKGHPVLLNRAPTLHRLGIQAF
                     QPKLIEGMAIQLHPLACTAFNADFDGDQMAVHLPLSNEAVLEAQLLMLASHNILNPAN
                     GAPITVPSQDMVLGLYYITKPRPNALGEGLIFYSPEEAEIAYNEHKVDIHARVKVRTK
                     DLDENGNSVEKLIETTVGRIIFNKYVPEEVGYINELLSKKSLRDIIGRVIETCGMART
                     AHFLDDIKELGYNMAFRGGLSFNLDDVIIPKDKEKLIEEGNREVEEILNNYNMGFITY
                     NERYNQIIDTWTHVNSKLSDVLMKELSSDNDGFNSVYMMLDSGARGSKEQIRQLSGMR
                     GLMAKPQKSVSEGGQIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKTADAGYL
                     TRRLVDVAQDVTITEEDCGTLRGLIATEMKNNEEVISSLYERILGRVSVHDIYHPLTG
                     ELIVSSGEEITESLAKKIQDSPIEQVEIRSVLTCESKKGVCAKCYGRNLATGRMVNIG
                     EAVGVIAAQSIGEPGTQLTLRTFHVGGIASNIAAESKIVLRYDGRLEFDELRTVQPSD
                     DQSYRVVVSRLAEMRVVDPNTGIVLTTMNIPYGSKLYVKEGDICTKGQLICEWDPFNA
                     VIIAENEGKIEFEDVVENVTFKVETDEATGLREKIIIESKDRNKVPTAHILDKDGNIL
                     KTYNLPVGAHIVFENGDYIKTGQILVKIPRAVGKAGDITGGLPRVTELFEARNPSNPA
                     IVAEIDGEVTFGKIKRGNRELSITSKTGEVKKYLIPLSKQILVQENDFVRAGTPLSDG
                     SITPTDILQIKGPTAVQDYIVNEVQDVYRLQGVKINDKHFEIIVRQMMRKVEIIEPGD
                     TRFLEKQVVDKNDFMEENDRIWGKKVIVDAGDSPTLKPGQIITPRKLRDENSMLKRKD
                     LRPVQARDAIPATSNQILQGITRAALQTNSFMSAASFQETTKVLNEAAINAKVDKLEG
                     LKENVICGHLIPAGTGQRDFEKIVVYSREEYEKKVFARKSLIDVEEGEVVEENN"
     gene            complement(39977..43789)
                     /gene="rpoB"
                     /locus_tag="PLD44_01015"
     CDS             complement(39977..43789)
                     /gene="rpoB"
                     /locus_tag="PLD44_01015"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOF97952.1"
                     /translation="MSSSTNNQRINFASIKNQLEYPDFLEVQVKSFQDFFQMDTSSDK
                     RKSEGLYKVFTENFPIADTRNNFVLEFIDYYIDPPRYSIEECIERGLTYSVPLKAKLK
                     LYCTDPEHEDFDTVIQDVFLGPIPYMTPRGTFVINGAERVIVSQLHRSPGVFFGQSIH
                     ANGTKLYSARIIPFRGSWIEFATDINNVMYAYIDRKKKLPVTTLLRAIGFESDKDILE
                     IFNLAEEIKVNKSNLKKAVGSKLAARVLKTWLEDFVDEDTGEVVTIERNEIIIDRETV
                     LENEHIDLILQSGAQTILIHKEEANQNDYSIIYNTLQKDPSNSEKEAVLYIYRQLRNA
                     EPPDDATAREVINNLFFSEKRYDLGEVGRFRINQKLNLTTSMEVKVLTKEDIIEIIKY
                     LIDLVNSKAEVDDIDHLSNRRVRTVGEQLYNQFSVGLARMARTIRERMNVRDNEVFTP
                     GDLINAKSISSVINSFFGTNALSQFMDQQNPLAEITHKRRMSALGPGGLSRERAGFEV
                     RDVHYTHYGRLCPIETPEGPNIGLISSLCVYAKINELGFIETPYRKVNNGVVDLSENG
                     IGYYTAEEEEDLVVAQGNAPLDENGKFIRSRVKARLGADFPVVDPMEVHLMDVSPSQI
                     ASIAAGLIPFLEHDDANRALMGSNMMRQAVPLLRCEAPIVATGIEGRVIHDSRTQITA
                     EGDGVVEYVDANCIKIRYDRTEEEEFVSFDSAVKEYKLPKYQKTNQNTTIDLRPIVRR
                     GDRVHAGMIITEGYGSESGELALGKNLKVAFMPWKGYNFEDAIVINEKVVREDVFTSI
                     HVVEQLLDVRETKRGTEEFTSDIPNVSEDATRNLDENGIIRVGARVEPGDIIIGKITP
                     KGESDPSPEEKLLRAIFGDKAGDVKDASLKATPSLSGVVIGKRLFSKAQKNRKSKLAD
                     KAVLPKLDEEFETQANALRETLIEKLMKILTNKTSTGVKDFLGTDIIPRNSKFTVERL
                     REIDYTTVQLGKWTNDAHTNDMVNSLITNFLRKFKELDAQLKRQKFNITIGDDLPSGI
                     MQMAKVYIAKKRKIRVGDKMAGRHGNKGIVSRIVREEDMPFLEDGTPVDIVLNPLGVP
                     SRMNLGQIFESVLGWAGKELGVRFATPIFDGATLNDLNAWTDKAGLPRAGKTYLYDGG
                     TGERFDQPATVGVIYMMKLGHMVEDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEME
                     VWALEAFGAANILREILTVKSDDVNGRARTYEAIVKGDPMPTPGIPESLNVLLHELRG
                     LCLSINLE"
     gene            complement(43901..44278)
                     /gene="rplL"
                     /locus_tag="PLD44_01020"
     CDS             complement(43901..44278)
                     /gene="rplL"
                     /locus_tag="PLD44_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOF97953.1"
                     /translation="MADLKAFAEQLVNLTVKEVNELAQILKNDYGIEPAAAAVAVAAG
                     PSAAAAEAVEEKTAFDVVLKSPGANKLAIVKLVKELTGLGLKDAKDLVDAAPSVIKEG
                     ISKDEAEALKNQLVEGGAEVEIK"
     gene            complement(44317..44832)
                     /gene="rplJ"
                     /locus_tag="PLD44_01025"
     CDS             complement(44317..44832)
                     /gene="rplJ"
                     /locus_tag="PLD44_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOF97954.1"
                     /translation="MKKEDKSTIIDQIESVLSEYPHFYLTDIGGLNAAQTSELRKLCY
                     KEKVKLVVVKNTLLKKALDRSSVDFSELYPTLKGETALMLSDMANVPAKLIKNFSAKN
                     KKPVLKAAYVEESFYIGENQLDTLVNVKSKNELIADLIALLQSPAKNVVSALQSGANT
                     IHGVLQTLSEK"
     gene            complement(44844..45539)
                     /gene="rplA"
                     /locus_tag="PLD44_01030"
     CDS             complement(44844..45539)
                     /gene="rplA"
                     /locus_tag="PLD44_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOF97955.1"
                     /translation="MSTLTKNRKYALEKIEAGKAYPLIEAAKLVKEVTTTKFDASVDI
                     DVRLGVDPRKANQMVRGVVSLPNGTGKQVKVLALCPPELEAAAKEAGADYVGLDEYIE
                     KIKGGWTDVDVIITTPAVMGKLGALGRILGPRGLMPNPKSGTVTNDVAKAIKEVKQGK
                     IDFKVDKFGIVHASIGKVSFTAEQIAENAQEFISTLIKLKPSTAKGTYIKSIYLSSTM
                     SPGIKVDPKSIEI"
     gene            complement(45561..46001)
                     /gene="rplK"
                     /locus_tag="PLD44_01035"
     CDS             complement(45561..46001)
                     /gene="rplK"
                     /locus_tag="PLD44_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOF97956.1"
                     /translation="MAKEIAGVIKLQIKGGAANPSPPVGPALGSKGINIMEFCKQFNA
                     RTQDKAGKILPVVITYYTDKSFDFVVKTPPVAIQLLELTKLKSGSPEPNRKKVAEISW
                     DQVKEIAQDKMVDMNCFELESAMRMVAGTARSMGITVKGTFPGK"
     gene            complement(46055..46603)
                     /gene="nusG"
                     /locus_tag="PLD44_01040"
     CDS             complement(46055..46603)
                     /gene="nusG"
                     /locus_tag="PLD44_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOF97957.1"
                     /translation="MSDTTNFKWYVLRAISGKENKVKEYIETEIKNSDLGQYVRQVLI
                     PTEKVFQIRNGKKIAKERNCMPGYVLIEANLMGEALHRVRETPNVLGFLGEKNNIPTP
                     IRTSEINRILGTMDELQESEEEMITPFMVGENVKVIFGPFSGFSGTIEEVNTEKKKLK
                     VMVMIFGRKTPLELAFTQVEKE"
     gene            complement(46629..46820)
                     /gene="secE"
                     /locus_tag="PLD44_01045"
     CDS             complement(46629..46820)
                     /gene="secE"
                     /locus_tag="PLD44_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOF97958.1"
                     /translation="MKIINYIKECYSELVHKVSWPSLSELTNSAIVVLIASIILALIV
                     WVMDLSFESIMTFIYEKIF"
     gene            complement(46836..46912)
                     /locus_tag="PLD44_01050"
     tRNA            complement(46836..46912)
                     /locus_tag="PLD44_01050"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46876..46878),aa:Trp,seq:cca)
     gene            complement(47039..>47739)
                     /locus_tag="PLD44_01055"
     CDS             complement(47039..>47739)
                     /locus_tag="PLD44_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HOF97959.1"
                     /translation="DGDNTPVIRGSALGALDGDPKWEAKIVELMDAVDNWIELPQRDV
                     DKPFLMPVEDVFSITGRGTVATGRIETGIVKVGDEVQIIGLGHEAKKTVVTGVEMFRK
                     ILDQGQAGDNVGLLLRGIDKDEIKRGMVITHPGAVTPHKKFKAAVYILKKEEGGRHTP
                     FHNHYRPQFYIRTMDVTGEITLPEGVEMVMPGDNIEFTVELIYPVACSVGLRFAIREG
                     GRTVGSGQITEILD"
BASE COUNT        14214 a        10028 c         8739 g        14758 t
ORIGIN      
        1 attaacggga aagtgttttt gcagtcctat tacaaaggat tattggaacg ttagcaatta
       61 ttttcatttc caaaaattaa gtttccacta acaatttttt gaaaaaaatt gatttttcac
      121 aatcgacaaa tagctgaata tcagtcaaaa aaattgaaat aaaatttatg gaataatttt
      181 ctcaggagcg cgtaaacttt ctttttctga taagttgaaa tacaaagccg aaaatcgcaa
      241 gaataacaag cggtagcaac acgttgacaa gttgccattt tagtttttgg tcgtaaacaa
      301 cctgtctgtt gagcaaacga agttttaatg ttttgttttt cagttgcatc catccttcat
      361 catcggcaag gtagagcact gcattttcaa taaaatccct attgccgtat tgcaaacctg
      421 tcaatctgtc gaaacccaaa ggcaagtaga tactgtcacc ctgaactatg gaaatttcat
      481 ttttgataat atctccatct gccacaaaaa tttgttttgt gtttacactt acaggacgaa
      541 tactcgaaat gttcaccacg ctatcgggca acattcggtt ggtaaaaacc gattcaaaat
      601 tgccttccaa aagaacacca actggaacat atccagtatt gaaataattc ttgtcgtttg
      661 cagatggcat atcccgaaga tcaacagtgg ttggagttcc aatcacatga gtattgtcgg
      721 aagttgcaag tagcaaaacg gctgaaactt gttgattctc accaaccaaa tcgatggcag
      781 aacaaaattc ggagcgaacc gtcgcaaggt tacgggtaac gggatgagca taagaagtga
      841 gcaaaagtgg agcaaaaaac cagggaagag gttcgaaatg aggagcttct tttgtttttg
      901 ccacattaac aggcatttgt gcacattgaa catcttgcaa aataaccggt ttgatacgaa
      961 ttccatatcg aaaaagcagg tcggaaagat tcaaatcgag cggaatggca ggtgatgtgc
     1021 cggtaacaga aaaattttct tcagccaggc gcaccccatc aatcaaccaa agcacttttc
     1081 ctccattcat aatgtattga tcgataatca gcttttcttt ttcactgaaa ggaagttgag
     1141 gtttggcaac aattaccact ttatagggat ggagaatgga cgcatcattt cctatgcgac
     1201 ctcgatcgat ttggaaataa cggctcaacg atttgctaat gtcataagtc gcagcttcgg
     1261 acagttcgtc atgtccttcc agaaaagcaa ttttatcaac tgacgtgtta accaaacgac
     1321 ggacagcatc cgtaatttca tactccagtt cgataatgga aatattcaga ttctcgtcgc
     1381 ccgaattgcc gggaatgttt ttcaacagat ttaccaaaac cttgcgtccc ttgtattcca
     1441 tttccaacca cggaaaaatt gttttcctga ttattttgcc gtcgttatcg cgttcatata
     1501 cattggttgg tgtcaatccg cgcgattcca attcggcata atgcttttct ctttcttcag
     1561 gcttgacaac agatgaagga tcttcaaatt caattgctat gggcttggaa ctgtaaactt
     1621 tcagttcttc caacatttcg accgtagatt ttttcaacct taaaaagccg gaattgagac
     1681 tgccatcgag ataaattttt actttcagcg gaaagtcgag ctttttcatc agttttttgg
     1741 atggttccga caaactgtag cgcttttcgg acgtcagatc tactctgtaa tgaacgaagg
     1801 aaagcaccag cgacaacagc aaaaaaactc cccaaaaaaa taattgcttg tgttgaatta
     1861 tattacgcat gttacagaag attcttcagt aaaaatcgag aagaaattcg gatgcaaaat
     1921 taatcatttc ctttgagcaa aaacgcatcc tgaaaaaatt tggtattcag aatcttttct
     1981 ttaccaatac tttcaaaccg gcaggtacaa cccggatatt tatttcctta ccttcttcat
     2041 agggctcgcc atcataatga aaagggccgg gcgcaggtcg aaaaaccgta atttcactca
     2101 ttcggaaagt attgataaac aagtcgttgc ttatagtctt ggtaaaaagt tgcagtgcaa
     2161 gagtaggaag agcaataatg gggaaattac tcataatgga aacatcgagc cgtccatcct
     2221 gaacgctggc ttttggggca atgtacacat tatttcccca ttgagaagca ttagcgatgg
     2281 tcataagaaa agttttggtg gtaaaatcga tatttttccc aataatacgg taattttcag
     2341 aatgataggc aaaataacct ttcacaattt tttccagata agccacaatc cctctgcgat
     2401 ggcctttggc aaattcggca gcaatgtaag cgtcgaaacc ggttccgcat gtgcagaaaa
     2461 aaggtcgttg atttaccaat ccgtaatcga tgaaaatttc ctccgaaaaa ttgagcattt
     2521 gaatggcttt tcgcatattc attgggattt ccaaatgtcg cgccaaacca tttcccgaac
     2581 caatgggtaa aattcctaac gaaaccgaag tttgaaccag tccgcgggca acttcgttaa
     2641 tcgttccatc accaccaact gccaccacgc tatcgaaacc cttagcagca aaatctctgg
     2701 ccaataagct tccatgacca cggtagtcgg taaacgccac agttggatca aacaaatctc
     2761 tgtttaattc gtcgtggata tgttgcggga tgaaggactt tgtccttaca ccggccatag
     2821 gattgataat gaaagcaatt ttctttttca tggcagaaaa acgtgcgttc aaaaaacggc
     2881 tgcgaagtta caaaaaacta ttaaactgca aaattcatca ataattattc aacattatag
     2941 aatccggtta agttcaactt agaagtgcga attaagcaaa aagttttaca ttgtttttta
     3001 ataaacccaa agcaccacag ctgataggca aacgactgga aggaagtaaa agcttgaaag
     3061 agcattaaaa tgaaccgaca acataattca ccaaaaaagg agaccgaagt cttcctaaaa
     3121 ttaatactgt ttatgaaacc cacatgtatt gcatacacaa acacgaatga atataatatt
     3181 tagacatgtg ggttccatca gacctttaaa aaataaatta tattctgatg aaagacaaaa
     3241 atttgagcaa tcggtagtcg caaagtttct cggcagagaa aatgacgaaa aaattacttt
     3301 cttttttcga gttgtttctg aatctttttt attgctgatt ctatgggttt ttcgggttca
     3361 atcacattgt aactgcccca aaattcagga tcagaaaagc cggaagcttc atctgcaata
     3421 attaccgtcg ggcgcaactt gtttttcaat ttcttttctt cagtagcttt ttcccaattg
     3481 gtaacggcca tttccactac cgtatagtaa ttggaattga ataattttct cttccagtca
     3541 attttaaaat ccaattctat ccgactgtag ttatagtacc attttccatc cttttcaatg
     3601 taatccattc gatatgaggc atttgttaaa aacacttttg cattaagggg ttttttgcgt
     3661 atcagcatgg aagctgattt ttctttgtcg gtaagatcaa gatgaaaaat ggcacttctc
     3721 aaggcaagac tttcagcatc aatgtaaagt tttccataat ataacggaat gttgacgaat
     3781 ggccgttgct taaagttgac aatataaatt ggctgatgat cgatgtaagt agtccgatca
     3841 aaagaaaatt cataattatc caagatactt tcagtaaaaa gatagtcggg atttttcatc
     3901 acatccatgt aaagcgaatt gtaaggacca cccatcaact taaaaaccaa tgtatccatt
     3961 tttttatagt cggattgttt cctgctttta tacaattctg cctgatcctg ccggaatgaa
     4021 gaataaggct gtttaagaat atccacaatg gcttcagaaa gcgatacata agagcgattt
     4081 tttttgatgg tttctctgta aaaagctgtc attaagagtt cttcttccgg ataatttttg
     4141 tagcgatttt ccaatactga cagtaccaaa ttttctgcat ctgttgaaat cacatttatc
     4201 tcgggcaaag aaacaatcat gggttccaaa gttatgcgag aattttcctg ttttagctcg
     4261 gaaattttaa gaattttgtt tttgtatcct atataactga ccgttagtgt tttatcttga
     4321 aattcttttg gaattttcaa ggaaaactct ccttcggaat tggaaaccgt agtgatattg
     4381 cttccttcta ccgaaagagt ggcaaatggc aatacatctc cgtttttaac atctactact
     4441 ttacctgaaa attccacaaa atcagaatcg ttcttctgaa aatttttttg aaaattattt
     4501 tgcgatggca agcaaacagg gagcgataac aaaactaagc tccataattt aaacgggaaa
     4561 gaatggaaat ttttttttgt tttcatggcg gcaagttata aagagtagaa ccggataaat
     4621 tgttgaatct acgtgaaaaa ttcttacaaa caaagataac acattcaaca tggaattgtg
     4681 ctatatttca cttaaaaata acaatttggc tggacaaaag tttgagacat caaatgccgc
     4741 aaaaacaaat aagaaaccca catgtattgc atacacaaac acgaataaat ataatattca
     4801 gacatgtggg ttccatcaga ccttaaaaaa ataaattata ttctgatgaa aaattgcgaa
     4861 aaaaaccaaa aattatcagt gagctatcat taactttatt gtttttcgct gatttccata
     4921 atatactacc aaaaggtacg aaccaatttg aagttcggaa acatccaatt tcccatctga
     4981 agagaaatta ccgattttca cacttattcc gctgaggtcg taaatacgat aatatgaact
     5041 ttcgggtttg ttgaggttaa ccaacacaga agtcgatgcc ggattgggat aaaccgacag
     5101 gttcacatac aggtcattgt tgccggctgt tccgttccat acctcgcctt ttgatgtgcc
     5161 ccaaatgtat tctgccagtt caggatggtc aataaatgga ttccgattct gttgaattcg
     5221 ataaacacca tcattccggt tgatttcctt gtcgctgacc gggtcatttc tgctccactc
     5281 cagcaacatt tttatggccc attgtttgaa tacgggataa gtatttctgt ccaacatgct
     5341 cgaagttccg gttcctctcc agtacgaatg ataatcttcg taacaggtaa ccatatacat
     5401 gtaagttcgt gcaaaatcgc ctttgtattg attagccggc tcaaaaacca acccggtgta
     5461 accgggataa gtattgtttc caacttttac caccccatta tcgaaactgg cagtaccaac
     5521 aattcccaat ggataattag atttggccga gttggcttcg gcatccgaag gagaaagatt
     5581 caccaaatcg ccattggcat cgaatgttga ataattcgaa cttgttcccc accagcttcg
     5641 cggcatgcag tgttcacgat tcataacgct gctattataa gtggaacgtt tgttgttgga
     5701 atacatgtcc cagaaataac cgtccggatg ccagtcggtt gtcttaaaat aagtgtacca
     5761 ccaaacggta gtactggtgt cgtaattcag aataacgtgt tgtcgaatga tattgtgcaa
     5821 ggcagttttt aattcagccc ctgtttttcc ttcagcagtc cggtaataac cttcaggtat
     5881 ttcggccgaa agagtaaggc tgaaaaacgt cagaacaact actgaaattt ttttaataaa
     5941 gcaaaaagaa aatttcatgt actaagaaaa atttatttta ttaattttcg tgcaaaagta
     6001 aaatctttat ttgagatacg aacaataaaa atccctctgt gcggaattct tatactcatc
     6061 tccgatcctg atgaatgttg atcaaacaaa catacgccgg caatattaaa aagttgaaca
     6121 cgctcattgc cttgcaagtt cgacaattta atatcgccac tggatatgta aattttatat
     6181 tggcttgcag gtatattttc caaagctgaa ggaaatccgg tcttcacctg aatagtttca
     6241 gaaaaatccg atgtatatgg atgtatggtt gaacgaaccc gataaaaata aactgaattc
     6301 ggattcagcc cttccacttt aaattgactt cccgaaacct gaaaattttt caacacaaaa
     6361 actgtgtcct gttgcttgta agttgcctcc acatcgtcaa gtgccaggtt gcctgatact
     6421 tttttgtaaa taaacctaaa ggaatgaata tcatccgatt cctgaaaagt gtacatcgga
     6481 taattttttg aagtatttac cgatccaatg ctgtcgatac gaacataatt tccattcttt
     6541 actgcttcga caataagcga tgaaccggtt gctcctgaag ggaaacgata cataaatttt
     6601 agttgcgaaa cccagctcgg ataagttttg gtttgtaact attctccatc atttttaaat
     6661 gccaacgcgt tgggattttt accggagctg gcagcggtag tataatttcc gcttgctgtt
     6721 ccactccacc cttcaggcaa aggtttcccg ttgcttccaa tattgtcaaa cgattcggtt
     6781 tcagttgtat ctttgccgga ctgtatttga aaaacatcca gcagataatc ggacgtgtac
     6841 aggcaatctt cccaaccggc aacaaaactg ttggctcgaa catccgttgc attcataatt
     6901 acaggcactt caagtttgga agtatgaatc tgcatggtgt tcaaagcttt tttttcttca
     6961 tcgggatata ttgccgttac ggaataagta taagtatttt cgggtaacaa ttgcgatatg
     7021 gtgaaatgat ccgttttccc ggtctcaaaa tcatttaaca gatagttgga ggacggttgt
     7081 aagttcattt gatgatttcc aagcatttga aaatcatcta ttccgtaaaa attccattct
     7141 atatctctga attgagaact tggatgcgta accgtagctt ttcgtttcaa agttaaatcc
     7201 tgaccccaat atacatcagc acctgcattg gcgatgccta caacatcaat aaaaacacca
     7261 tttctcaaca aagcaacggc atcgtttcca ttaaaactga gtaatgaatc cgttaacaaa
     7321 ttggcttttg ctttcaaatc gttgtttgaa cagcttttgt gaaccaacac ataagttttt
     7381 ccgttttcca gcattccatt caatttcagt gtagctccaa aatttccatc tccattggat
     7441 tgtttttgga gactgtaatt ggataaatcg atatttttcc cggttccatt gaacaattcc
     7501 aaagctttgt tccagctact gccttccaca taagcggaaa taatcaaatc gccggcttgc
     7561 tgatcgcctt gatacagatt cagtttgtag ttgtcggcat cggggtcgaa aatccatcgt
     7621 aatgttcctc ccgtaggagt gatattttgc ggttctaagg caagaaaatc agtcgaagcc
     7681 aaaccttgca gtgggatacg caaagaatct ttccaaccgt cggaagtcag caaaagcgta
     7741 tctctgttcc agcctgaaac cacaggacga aaagtgataa ttacttcagt gccattgata
     7801 acttcttctt tcgtcaacga agctttggaa attttaaagg atttactttt gcccttaagt
     7861 ctgatattca aattgttttg taaattcaat cctgaaacag ttaaatgctg ttgaatggaa
     7921 tcggtttgca atattacgcc gaaatccatt tgatcaccgc gtttgggata aatcagaaac
     7981 ggctctgttt cttcaggaaa aggaaaaaac tttgttgtat cggcacccca aatatattct
     8041 gccaaatcgg gataatcaat aaaaggattg cgatttccct gaatgttgta aacaacttca
     8101 atacgatcgc gttctttttg actgacaggg tcttctctat tccatttcag caataaatcg
     8161 atagcccatt ttttccacac cggataagtg ttgttattca tcatgggcga attccacaag
     8221 gtagcaaaat tttcgtaaac agttgacacg tacagataag aacgcgcaaa atcgccttta
     8281 tattcatcgg caggctcaaa gcaattgtcg gtataaattc ctccaaaacc atttttccca
     8341 attttgctta ccccattgtc gaaatttggc gtgccggtaa cttccccaag cggaagattg
     8401 ttttttgtag aattggtaac tccatcggcc ggatacaaat gaaacaaatc cttgtatgca
     8461 ttattttcgt gtccgcccca ccaacttttt ggcaatgagt gctcaatatg catgcctgcg
     8521 acggaagaaa aatccttgaa gtaccgtatt tccgaagaat acatatcttt taccgaacca
     8581 tccgggtttt gatcggcata ataaaaccct tcccatgtat atcctttccc actgccataa
     8641 ttgaggacac gcaatggaga tgcaatttga gaaagagctg attttaattc tgccttcttt
     8701 ttgttgtggg ctaaataata ataaccagcc ggcacttccg aaaatgcaat ctgacagttt
     8761 agccaaacaa ttaaaattaa aaaaagcgtg ctgaaaaatt ttttcatttt ttcgttcaaa
     8821 ttaaatgcga gacgttttta agatcatcct tatttatcat cactattttg taaaatataa
     8881 aagttgctgc cattacctgc tgaccataaa tttttgaatg gtttttttcc catctttctc
     8941 tagttgcagc aaatacattc cgttatttag ttgagataca gatatagaag aatttccatc
     9001 aatcaaggtt ttcattaatt cctgcccctg aatgttgaaa atagtacaca ttaattcgtt
     9061 ttcgtcagtc ttgatattca gaaaatcttg agccggcaca ggacttatgg aaaaatctat
     9121 tttcagattt tcaattcctg aagaaagcga ccaaggttca ccaactttcg tgccccaaat
     9181 atattctgcc aactccggat gatcaataaa aggattcctg tttttttgat aaacttctac
     9241 ggcattgttt ctgtcggttt ccttttttga aaccggatcc tgccggtgcc atttcaaaaa
     9301 taatgctttc gcccaatccg agagcgccgg ataagttgtt ttattaaatg aatctccatc
     9361 aatggtagtc ataatgttct gataacgtgt ggcgaaataa aaataagtac gtgcaaaatc
     9421 gcctttatat tcatcatcgg gttcaaaaac tgttcccgaa tatcctgtaa aagtggaatt
     9481 tcccaatctt cctcttccac cggaaagggt aattccaccg ctacattcgc cataagggaa
     9541 attgcttcgc tgcccattca ctttcccatc ggtgggataa acatgaaaag catcgcttac
     9601 cataggcgat tgagaattaa accagctttg cggtatggaa tgttcgcggt tgtaacaatc
     9661 gcaaactttg ctataattcc cgcacttttt ttgtccgggc acccaagtgc agtcggaata
     9721 catgtcccaa ataagacctt cgggagtaac atcgctggtt tcgtaaatgg tgtacagaaa
     9781 atcataactt ttgcttacat aacctgatga aataatggaa taaagagtgg ttttgagttc
     9841 ggcaccgctt tttcctacgg cctgattgta ataaccctgc ttgggttgag aaaaaacaac
     9901 tgtcagattc aaaaaaagca acaacaatgg gagaaagttc ttcttcatga tgaattaatt
     9961 caattaaaca aatgagtaaa tgagttttta tgcaataaac aaataaaaat gtttaaaaaa
    10021 tgaatattta taaaatttcc taaaaattta gggcttcaaa gataaaacat tatttttaaa
    10081 tggcaagtta aaaaaagaca atagaaggct gtagagttaa tcggcaaatg tgaaattaat
    10141 aaaatgtttg aaaaattcat tctaaggaaa aattaaaacc tataattcag gtacattcag
    10201 ctaaaattga ccaaaataac gtgaatattt aatccgaatt tttttttaaa aggtttattc
    10261 aaaaaatagt atgtaactag caggtataac ctgtttactg aaaaaatatt atctttgtat
    10321 tgatgtttca agaaccaaaa agaaactgta gaatacgtca acgacatgga gtttcaagaa
    10381 aaaatgatgc gtaaagcaat tgcattgtct ttggagaata taaaaaaagg aggaggcccg
    10441 tttggtgcgg taatcgtgaa agatgggaaa atcattgcaa ccggtgtcaa ccgtgttacc
    10501 tccaacaacg atcccactgc acatgccgaa ataacggcca tcagaaaagc agccggcaaa
    10561 ctgaaaaatt ttgacctgtc gggttgcgaa atttatactt cgtgcgaacc ctgtccaatg
    10621 tgtctgggtg ctatttattg ggctcatctc gacaaaatgt attttgccaa caccaaagcc
    10681 gacgcgcaaa atgccggatt tgacgattcc ttcatttacg aagaaataaa tctgaagcct
    10741 gaagacagaa aattaaaatc gcaacaactg ctcagagagg aagcccttgc agcatttgaa
    10801 acatggaaaa acacaacaaa taaaacagaa tactgaaagt tgcttttggc aactgcattt
    10861 ataatagtta atttgcatat tgaatatctc tgaatttcaa caactttatg ctcatcaccc
    10921 gcagatgttg cgggtaaaag aatgggcaaa ttcactcgaa cagaatttaa aaatttccgg
    10981 tttgagcggt tcatcatctg cacttgtttt ttcgtcattg ttcaaagccg ttcctcagca
    11041 ccatattata ataatgaatg atgccgacga agcaggatat ctgtacaacg acctgaaact
    11101 gttgctgtct gaaaacgaga tttatttttt cccttcctct ttcaaaagag ccatcaaact
    11161 gtcgcaactc gacgctgcca acgatatttt aagaaccgaa gtactgaacc gccttgccaa
    11221 taattctcag ccgtgcattg ttgttaccta tccagatgcg atcatgcaaa aaattgtttc
    11281 gcttcaaagt ttgcgtcaaa aaaccttgaa acttcacatc ggcgaaaata tcggtatcga
    11341 ctttatcaac gaattgttgt tcgactttgg ttttcaacgg gttgacttcg tatatgaacc
    11401 cggacaattt tcgattagag gaggaattgt ggatattttt tcctttgcca atgaactgcc
    11461 ctatcgatgc gacttttttg gcgacgaaat agaatccatt cgtgttttcg atatcgaaac
    11521 ccaactgtcc aaagaggaaa agcaagaaat tgaaattgtc cccgatcttc agcgggaaaa
    11581 taaattgcct catgtttcct tttttgagtt tattcctaaa aataccctgc tgagtttcac
    11641 gcacccaaat tttattcatg accgcataaa tcaactttat gacgaatttt tggtaaaagc
    11701 aaattccgaa ggacatgaaa cgccgcatct tcacaaaaca atgattaccg gagaccagtg
    11761 gttggaactc attgaaccgt ttagaaaaat aaactgggga accgatagaa cttttttcaa
    11821 aacttcgagc caaatttctt tcaatacctc accacagccg gtttttcata aaaatttcga
    11881 tttgataagc gatcatttga aaaagaaaat cgaagaaggt taccgcattt acatcatgag
    11941 cgacagcgtg aaacaaacgg atcgcattgc tgccattttt gccgatcgag gcgacaatat
    12001 ccattttcaa cccattaaat ccactcttca cgaaggtttt gtcgaccacg acctttctct
    12061 tttgtgttac accgatcatc agattttcga ccgttttcac aaataccagt taagtaccga
    12121 taaaacccgt cagggaaaag tggtaatgac cttaaaggag ctcaatcagc tgaaaattgg
    12181 cgactacatg gtacatgtcg atcacggtat cggagttttt ggcggcctgg tgcgtacgga
    12241 agtaaacgga aaaatgcagg aaatggttaa attgatttac aaggatgacg atattctttt
    12301 tgtcagcatt cattccctgc atcgcatttc aaaatacaag ggaaaagaag gtgaatctcc
    12361 ccacatcaac aaattgggct cgggagcttg ggagcggctg aaagaaagaa ccaaatctaa
    12421 agttaaagat atagcacgcg aactgataaa actttatgct caacgaaaag cagaagaagg
    12481 atttcggttt tcgcccgaca cttatcttca acaggaattg gaagcctcat ttatttacga
    12541 agatacaccc gaccaaacca aagctacggt tgaaataaaa aaagacatgg aatcgacttt
    12601 gcctatggat cggttgattt gtggcgatgt cggttttggc aaaacggaag tagccatcag
    12661 agcagctttc aaggcagttg ccgacaacaa gcaagttgcc gttctggttc cgacaaccgt
    12721 gctcgccgta caacactaca atacattcaa agaccgatta cacgattttc cctgcaatgt
    12781 ggaatatctc accagagctc gatcggcaaa ggaaacaaaa gaaattctcg aacgtttggc
    12841 caaaggtgaa atcgacatca tcatcggaac ccataaactg attggaaaat cggtaaaatt
    12901 caaagatctc ggattgctga ttatcgacga ggaacagaaa tttggcgttg cagtgaaaga
    12961 aaaattgaaa cagatgaaag ttaatgtgga caccttaacc atgactgcca cacccatccc
    13021 tcgaacctta caattttcgc tgatgggagc cagagacctg tccatcattt ccactccgcc
    13081 gcccaatcgt tatccggttc aaaccgaaat tcaccgtttc gacgaagatg taattcgtga
    13141 agccattcag ctggaaatga acagaaacgg acaagtattt tttgtacaca atcgcataca
    13201 gaacatttat tctattcagg ccatgataaa aaggcttgta cccggttgcc gcgttgccgt
    13261 aggacacggt caaatgccac ccgacgaatt ggaagaagtc attgtcgact ttatcaatta
    13321 cgattatgat gtgctggtag caaccactat catcgaatcc ggcatcgaca ttccgaatgt
    13381 caacaccatt attatcaaca atgcacatac tttcggactg agcgatctgc atcaactcag
    13441 aggaagagta ggtcgcagca accgaaaagc tttctgttat ctgctgacgc ccgaatttca
    13501 attgcttact accgaagccc gacatcgact cgaagccata gaaacttttg ccgatttggg
    13561 aagcggattc aatattgcca tgcaggattt ggacattcgt ggcgcaggga atttgttggg
    13621 agcagagcaa agcggattta tagccgacct cggttacgaa acttatcaga aggtactcaa
    13681 cgaagccgtt caagaactga aagatgaaga atttgccgac ctgtatgctg aagtggaaga
    13741 aaaaaccaca actggtggta cttatgtaac cgattgtcag attgattccg atttggaatt
    13801 actgtttcct cccgattatg tggaaaatgt ttccgaacgt atcaatctgt accgacagct
    13861 cgacagtatt gaaaacgaaa accaactggc agcttttgaa aaggaactgg aagaccgctt
    13921 tggtaaaatt ccacctcagg gaaaaagcct tattttgatg gtacgtttgc gatggatggc
    13981 cataaatttg ggtatcgaaa aaatggttct gaaaaatcaa caaatgacga cgtatctcgt
    14041 ttccaatcaa aaatcccatt actaccaatc cgatactttt ggcgaaatac tgagatatgc
    14101 tgcttcgcat ccgcatcaat gtcaactccg tgaaaaaaac ggacgtcgct ccattatttt
    14161 cctcaaagta acatcggtag aaaaagcttg ggaaattctt tcagaaattt atcgcaacct
    14221 cgaaacggca aaattacaaa aggcggtata aacaaagggt agaaaaaacg aaaaccgcac
    14281 tgtttgttgt gagaaaactc ctattgaata ggatttgaga cgttgacttt ctttgtaatc
    14341 tgccgttcgt cattgctgac gaatacccga aggcgcagcc cgccattgaa agtcagactt
    14401 ttctcggttt gaattcttta ctgatgagtt ttccagtctc taaacaatgc ggctcgtttt
    14461 tctttaatgc aaatataaaa cattattctc gatttgatgc cctttttcat agaaaatttt
    14521 gttaaaaatg cagtttttcg cctttgtcaa caaaacaaac tgataatcaa accgataatt
    14581 ttttcttctc ccaaaatgta catttcttcc ggctttcata tattttaaaa ttttgtaatt
    14641 ttagagcaga aaaaattgaa acaatccttt atcggatttt agcaaataaa ataaaatgaa
    14701 acttttcgaa caactcgaac cacacagaaa cgattatttt ggttcctttt ccgttggtac
    14761 gctcgaattg gttttcaacg actggtacca aagaaaaaag aaggtaaaaa cattacaccg
    14821 ttttaaagtg gaatagaaca aatttcctcg ccaacaaacc tatctctctg cccgcaagtt
    14881 gatgcacttg gaaaaaatga gacgaaatat ttcaaaactt tttggttttt cttctcagtt
    14941 ttccaaaatt aaacacgctg ataatcttat tgttgcaaat taattttgat aattaacaaa
    15001 atttaactta tgtatttgaa caactttata aaaaagcaat tacttttgtg gtgtactatt
    15061 taactattac actaattcaa taaacaattt attatgattc agatcaaaca tgtcgaattc
    15121 agctacagcc gcaacgctca attgtttacc ggtttggaac tgtcgctttc tcccggccac
    15181 attcacggca tactgggaaa aaacggggaa ggcaaaagta ctttgttgaa aatgatttca
    15241 ggcatgattt ttccacaaaa gggagaaatt gaagttctcg ggtacgaacc ccgaaaacgt
    15301 cagcccgaaa tgttgcgtga gatttttttc ctgcccgaag aattgccgcc cataactttg
    15361 agcatcgaaa actacgaaaa agtttatgct cccttttatc ctcatttcaa tcacgaacaa
    15421 tttcaaggat atttgcagga atttgagata aattcgggaa aatcgcaaat caacaaactt
    15481 tcgtacggac agaaaaagaa agtttttatg gctttcggat tggccaccaa caccaaaatt
    15541 gtattgctcg acgagccgac caatggtttg gatattcctt ccaaagccca attcagaaaa
    15601 atgacggctt ctgctttgga agaagaacgc attatgctga tttccaccca tcaagttaga
    15661 gatttggaca gcctgatcga cagcattatc attatgaact ctcaccaaat tgttttcaac
    15721 gaactgtgcg aaaacattac cgaaaaatta cttttcagtt acggaaaaga aaaaagtgaa
    15781 ggtgaagaag tgctttattc agaagaaaat ttgcgcggct ttgatcaggt tagtatcaac
    15841 aaaaccggtg aagaaagcaa actggatatt gaactgctgt ttaataccgt gctgaaaaat
    15901 caccagcgca tcagagaaat attttcgaat cagaacaata aacgttttta aaaattcttc
    15961 aatatgaatc agaaatttag tcttaaaaga atcggattgc tcttgcgccg atattttatc
    16021 gaaaatcaaa attacgaact ctacctttgg agcattttga ccattgtatt tatgattttc
    16081 agaaacggaa attctgcaag cgccatttta attgttacag gtgcacttta tgcagtaaga
    16141 tttttcaaag ttttcaattt tgcacccaat ggcattcaat atctactgat tccggctaca
    16201 caactggaaa aaacaatttc tgtgtttttg ctcaataccg tttactttat tttaatgttc
    16261 acggttgttt tttcggtagg aaacgtgatt ggaaatttac tggaacaact tattttcagt
    16321 acaagcgata cggttgcttt gcaatttttt caatcctcaa aaggttttga gtttaatatt
    16381 caaggcttaa aaaatatttg gatttcattc ggcgtttttg ccgccataca ggctattttc
    16441 actttggggt cgctgtattt caagcgaaac gccatactca aaaccttgct gagtctgttt
    16501 gttgtagccc tagcattggg catattggaa tattttattt ttaaaattac gtttggctcc
    16561 gttacagaaa ccctcaaggc cttgaatgcc ggtgactttg tatgggacga aaacatcttc
    16621 ccaaaagcac taacggagac atggaaaata ttttggtcta tatcgggata tttgtttata
    16681 cccttttgct ggatagtaag ttacttccgt ttaacagaaa aagaaattta aaatggaatt
    16741 taatgatgca caagctattt accttcaaat agccgattat atcagcgaac acattttgct
    16801 gggcaaatgg aaagaaggag aaagattgct gtcggtaagg gaactgggta cagaattgga
    16861 agtgaatccc aacactgtta tgcgagctta cgattttctg caaagcaacg aaattattta
    16921 caacaaaaga ggaatcggat attttgtggc tgatgacgca aaagaaaaaa ttctcaatta
    16981 tcgacgaaaa cgatttttgg aaaaagaact cccgattttg tttaaaaaca ttcatctgct
    17041 caacatcgat gttgaggaaa taaaaaaaag atataatgac taccttcaaa aacaacaagc
    17101 attatgaaaa caattaaaga aatgaagacg agcaacaaat tactggcggt cttttttatc
    17161 tatataatta taataatgat tatcaacaat ttaatcttaa aaagtcaaat aaaccgcatg
    17221 ttcgaatcaa cacaggaaca aaagacagaa accggtttag gcagccatga tgcatcgaaa
    17281 ttgtagggag tatttttttc agcaaaaaca aaaaaaagca ggttgtaaat tcgagatttt
    17341 ggaaaagaaa aattttcgaa aaacttcaaa acaaagatat aacgccctta ttctttgaag
    17401 ccacaaaaac ttttaccgcc aaaaattaaa tcttaatact gaaatatcag gctctttcag
    17461 aaaagatgac aaaaacagta aaattcgatt caaatttttc tctcatttta aatatcttgc
    17521 tctaatgaat gattgaagta ataacggcac catgacttta agccacatac attgcattta
    17581 ggttttctgg catgacaaac ataccgacca tgcaaaatta gccaatggtg agcaatgggc
    17641 aacaattgag gcggaatgtg gcgaaccaat tctcgttcag tctgcaatgg attttttgaa
    17701 tctgttgtca gtcccaagcg ttcggaaacc cgaaacacgt gtgtatcgac tggcatggca
    17761 ggctgattgt agatgactga aacaattata ttggctgtct ttcttcccac acccggaagc
    17821 gtttgtagtt tgtccacatt atccggaact tttccctcaa agtcagctac caattttcgc
    17881 gacatttcca ccaaatgctt agctttactg ttgggataag atacgctgcg aatatattcc
    17941 aataactttt caggtgtagt tgaggccata gcttctggcg tgggaaaatc ggagaaaagt
    18001 tgaggcgtaa ccatatttac ccgtttatca gtacattgtg ccgataaaat tacagctacc
    18061 aataactcga aagggttaga atagtttaac tccgtttgag cattggacat attttccata
    18121 aaccaatcta aaatgtgcaa ataacgttct tcagtggtca tgaaataaaa tcaatagttt
    18181 agaaaaattc tctttttatt gagaacaaaa aacgctatga aacccacagg tattgcatac
    18241 acaaacacga atgaatataa tattcagaca tgtgggttcc atcagacctt aaaaaaataa
    18301 attatattat gatgaaagca attttttaac catttccgaa atcgctttcc catctgcttt
    18361 tcccaccagc tgtcgagtgg caactcccat tacttttccc aaatcctgag gccctgtggc
    18421 accggtttga gcaatgattt ccctcagagt tgcttccagt tcgctttcgc tcatttgagc
    18481 cggcagatat ttttcaatga cggcaatttc tgccaattca ttttgctcga gatcgggacg
    18541 attttgttct ttgtaaatct gcgctgtttc cttgcgctgc tttaccattt tctgcaaaat
    18601 ttttatggct gcttcatccg ataatgttcc atcacttcct ttggcagtct tggcttccag
    18661 aaattccttt ttaattcccc gaagggcttc caacgtaact ttatcgtgcg ccagcatggc
    18721 agtctttatg tcggcactta ccttttcaaa tagctgttcc atttgtattt taaattagta
    18781 gatttgtctt ttttaattct cggaaaaaca cttttatttt cccttttttc tcttccaaga
    18841 aggaaatttt tcaatgtatt tcaaagtttg atcattttcc aaatcttcca ggttcactac
    18901 cggcaagtcc aattccggtt cttctatagg cttttcatcg ggaatggatt ttccataata
    18961 gctgtttacg gcattttcaa taattttttc cttgctttcc ggttgatttt ctttgggttt
    19021 taattcggag aaaacttcaa ttttgtcagg agtagtctct aacctttttt tatttttaat
    19081 aaccgatgca ggcatgccgg gtatatcgct cacatcataa ccggtggcaa taagggttat
    19141 tttaactcga tcttccaatt cgtcatcaaa agttgcgccc cagatgactt gaacattttc
    19201 accaactttt cgcatgaaat catggatttg ttgtacttcc tccatcttaa tttgatgagt
    19261 cgaagagcaa tacaagctca agagaacttt agaagctccg ctcacatcac tggtattgag
    19321 caatggggag ttaagtgcat tttcaatggc tttggtgatg cggttttcac ccgaagcata
    19381 accggtgttc ataatggcta ctttgccatc tttcatgata ctgtatacat cggcaaagtc
    19441 tgtgttgata taacccggga tggtaatgat ttctgctatt ccctttgctg cattggtcag
    19501 cacgtcgtca gctttggcaa aagcattgga cagttctaag tcgggataaa tttctttcaa
    19561 tttttcgttg ttgatcacca gaatagcatc tacatgttcg ctcaaagctg ctacaccttc
    19621 caatgcctgc ctgattttca gattcccttc aaaggcaaaa ggaatggtaa cgatgccaac
    19681 agtaagtatt cccatatcgt gagctgcttt ggcaattacc ggagaagctc ctgttcctgt
    19741 gcctccaccc ataccggcag tgataaatac catttttgta ttgtcctgca aaatgttttg
    19801 aattttgtcg atagattctt cggctgcctg acgtgcaact tcaggttttc cgccggctcc
    19861 aagtcctgat gtggtattgg cacccaactg aattttaaga ggaacaggcg actttatcaa
    19921 tgcctgattg tcggtgttgc aaacaacgaa cgacacatcc gtgattccct gacgatacat
    19981 gtggttgacg gcatttcctc caccaccgcc tacgcctatt accttgatga tggaattttc
    20041 tatttcggga agttccaaag gcaacatttc aactaattct tctatattga tcatatcaat
    20101 ttataatgtt cattaaaaat ttctcattta ttccaagtgg tttcatcatc gaaaaacctg
    20161 aggaaaccat cggtaattct ttctgtaatt tttttcttgt ttttttgtcc tttttttgtt
    20221 gtgttttccg attttaacgg atgaagacgt cgattttcgt ttgccagtaa aatagtgcca
    20281 ataagttgag aataagatga atcggccaaa taatcgggtg cttgatcttc caaccagtcg
    20341 gagtgattgc cccaacgtac cttcaaaccg cttttttctt caacaaattc ctttaaaaat
    20401 ttcagtttgg cgcctccgcc ggttatgata attcccgatt ccagtgggaa tgacaatact
    20461 tccagtactt taaaaacagg ttcggtcatt tcatccaatc ttgcattgat aatctgagcc
    20521 agaaaaagtg tggaaattct taccggatca gtgccggaag aaggagcaac ctgaacataa
    20581 acaggttttt cgacaaattt ttccattgct actcctgtca aacatttgag gcgttcggca
    20641 tttgattctt caatgccgag ttctgcaatg tcataggtta tgttcttgct tccaagcgga
    20701 acgaccagta aatcctgcaa aacatcattc ttgtaaacgg ccaatgtagt agtggaagcc
    20761 ccaaagtcta tcagcgcaca tccattctgc cgttcctctt cttctgtcag tacggtagat
    20821 aaagcttcca ctgaaagagg tgcatattcg atctgaattt gattgcgaac aaaacagctt
    20881 ttcaattgct gcaggatgcg tgcatttcct tttacaacat gataattgcc tgtaatctga
    20941 ttcccggatt gtccaacagg atcagttgta attttcccgt cgatttcgaa tgaaaccgga
    21001 attacatcaa acactacgtt gttttcatcg gaattttttc gttcacattc ttctttcatt
    21061 tcatccagca aagcatctgt aacaattttt ccctgaccaa taaaacgagt ggtcgaagat
    21121 ttggaaagtt tcatgctttt ggcattcaga gccacacaaa cttgttggat atcgcggatt
    21181 tttgcgctat tttgcaaaag tttcaaaagt tcgctgattt taaaagcagc gcctgaagtg
    21241 tgttcgataa tgccgtgata aacatcatcg gctgctttcg attctgtggc aagtaccttt
    21301 actgctccac tttccaatac ttcggcagcc atagcagtta tacgcgaact tcccaaatca
    21361 agtgctatat atgtgttctg attcataaat atttatctta aaaaaatatc aaaaaacttc
    21421 agccattgca tcaatcaata ttgtcggttt ttgtgcagac aatttgattt tcgtactgaa
    21481 gatcaattgt tttgtaataa ttccaaccaa attcattgaa ccctttttcg tagagtattt
    21541 ttagtttgtg gagttttttc tcataatctt ttaaattgcc cagcaaaatg atgccggttc
    21601 caacacgagg aacaagttca atttttctgt cggggcgaat gtaaatttgt tcgatttgag
    21661 cattccaaaa aggattttct tccaagtaag acacaaaacc gaacaattcg cctttcgcca
    21721 tcgattttgt aaccaatccc gaaactacag ggagacgtgc tgcatcgtta taggaaacgg
    21781 gaattatatt tttttcgtca tccacgtaga agttttcatt gcctgccagt ctgaattttg
    21841 gatgacgctg ttgcagaaat aaatacactc cacctgatgg cgttttgaaa cattcagctt
    21901 gtttgatgaa aggatgtttt gtcaataatt cttctatttc ttccgtcctt acatgtttga
    21961 gcgtttttcc cataggattc aaacctttaa tatcgatcat ttcggcaact tctttttcgg
    22021 agataagttt aacgcctgat tgatccaaaa aaacaatgtg gaacctgttg caaacttcgt
    22081 ttgcttttgg gtcggggaaa gtcaacaatg cccaagccaa atatcctgtt accagcagaa
    22141 taccgaatgt tatcaatata tatttgaaaa caggtttcat gactttattt cagtttcatt
    22201 atttaagaac gctttcaact tgttgaaaaa tatattttca tatacgcttc aacccaattt
    22261 cgattttttt tcagaaaatg ggaagatatt gttcatcaag catttccatt ggtttgctcc
    22321 atcagctgtt ttttaatgag cggaaccaac ttatctatgt cgccggctcc aaaagtaacc
    22381 aaaacttccg gatgcttgtc ctttaacaaa gataatagat tttctctgga acatagtgtt
    22441 ttattgtcca gtttaacatt acgaaaaatc atttcagagc ttattccttc aatgggttct
    22501 tctcttgccg gataaatatc cagcagaatc aactcatcca gcaatgataa tgcttcagca
    22561 aaatcggaag caaaatcacg cgtccgacta tagagatgag gttgaaatat tcccgtaatt
    22621 ttcttttcgg gaaataattc tttgatggac aaaatgctgg ctttcaattc ttttgggtga
    22681 tgagcataat catccatgaa aattgtttgt tccgaacgaa aaaccagatt aaatcttcga
    22741 taaataccag aataagaggc tattgctgtg cgtatttctt cttttgtaac gccattcaac
    22801 catgctaaag caatggcagc aacactattt tcgatgttga tgcgaatcgg aatttttaac
    22861 cgtatatccg aaatgcgttc ggaaggtgtt acaaaatcga aatggaattc atgattcacc
    22921 aaatgaatat tttcagcgta aaagtctcct ccgtcattca ttgagtaggt atattgcttt
    22981 actccttttt gcagacgcgg ttggatattg actccctttt tcatcaacaa aacgccacct
    23041 gggctgatga gcgacgtgaa atgctcaaaa ctctcgcgat aagcttcagg cgtttgatag
    23101 atatccaaat ggtcggggtc ggcggaagta atagctgcca tataaggaga aagttgatga
    23161 aaggaacggt cgtattcatc agcttctatt acgaacaaat tgctatcttt cgataacaaa
    23221 agattggagt tgtagttatt ggcaattccg cccaaaaaag cattgcagga tatatgcgac
    23281 tggtaaagca aatgagccgt caaagttgaa gttgtcgttt tgccgtgtgt tccggcaata
    23341 cagatggtag aagctttgcg ggtaatgtcg ccaagcagtt cagctctttt tttaatggta
    23401 aatccgtttt ctcggaaata aacgagttgt ggcagatcat cagggatagc cggagtaacc
    23461 accaccaatg tgttttcggg atatctgaac gagatgggaa tttgatccac attggggtcg
    23521 aaattgattt gtatcccttc cgcctgcaag tcggcagtaa cttttgactg aacgcggtca
    23581 taaccggcag tttgaaatcc tttggaattg aaatagcgag ccaaagcgct cattcccatt
    23641 cctccaatac ccaaaaaata atattttgta aaatccattt tctaaaatta aaattggttt
    23701 aggtcgtatt cgatttgtta tgaatcaatt cttttttgaa atcagttgca atacttctgc
    23761 cgcaattcgt tcggcagaat ctttctgcgc cagcttcctg atattttggc ccagtttatt
    23821 gagtgagtcc gcatctttta ttaactccaa cgctttcggt atcagcattt tttcggcctc
    23881 ttcgtctttt atcatgacag cagcttcctt ctgaaccagt gcaagcgcat tttttgtctg
    23941 atgatcttcg gccacattgg gtgaaggtac cagaatagcc ggcttcccca gcagacacaa
    24001 ttcggaaatc gaaccggcgc cggcacgcga tataaccaaa tcggctacgg aatatgccaa
    24061 atccatccga gaaacaaaat ctgtaacaat taattgaggc gacgaaaatg gttcggcagc
    24121 tttcctggct tcttcaatat aaaacttccc ggtttgccat ataatttgga tgtcggcttg
    24181 ggaaagttcc tgcaaatgcg atattatgct gcgattgata gttcgtgctc ccaaacttcc
    24241 tccgactacc agaagggttg gtttttccgg acgaaatccg aaaaattcaa aggcttcttt
    24301 acttttttcg ggtattgaca gcagttcctg acgtacagga ttgccggtta ataccagttt
    24361 ttctttaggg aaaaatcgtt ccattccttc gtaagccaca caaatgcgtg cagctttttt
    24421 ggccagcagt ttgttggtaa taccggcata cgaattctgt tcctgaatca gtgttggaat
    24481 acccaaatcg gaagcagctt tcagggtcgg ggcactggcg tatcctccaa ctccaataac
    24541 tgcatcgggt ttgaaatcgc gaatgatttg acgagccatt tgtaaacttc gccgaagatt
    24601 caacagcact ttgatgttgc gaaacgggtt ttttcggtca aaaccactta caggtaaacc
    24661 tttgatggga taacctgcag caggaactct ttccatttcc atccgaccca aagctccgac
    24721 aaaaagtatt tctgcatcgg gtagctgctt tttcagagcg ttggcaatac tgatggcagg
    24781 gaagatatga cctcctgttc ctccaccgct gatgataaat ctataaggca ttgttttaaa
    24841 ttataattgt cagtaaaact gaaatttaca tcaataattt tctaaaatgt tgtataaaaa
    24901 acaatttaca aaattaggat aattcgttca aatccacaac gggaaaattg cgttcctgtt
    24961 cttcaggtat ttcctcttcg acatgttgtt ctcctttgag ttgtcgagtt acgcctaaaa
    25021 taattccaaa atatatgcag gtgataatga tggaagttcc acctctgctt atcaacggca
    25081 aaggctgacc ggtaacgggg cccaaacttg tggctacagc catgctgaca aaagcctgta
    25141 aaactatcat cagggaaagt cctataacca gcaaggcagg aaataccgtt tcacttttta
    25201 cggcaatgcg gccggctctg taaagtagaa ccaaataaaa cagaatgatt attacccctc
    25261 caaccaatcc catttcttct acaataatgg cgtaaataaa atcggaataa gcttgaggaa
    25321 gataatccct ttccacactg ttgccgggca ttactcccaa cattccgcct cgggcaatag
    25381 ctattcttcc gtgttgcact tgccggtttt cgtccgtaat gacatatttg ttttcttcct
    25441 gtttcttttc ttcggtgtat cggtcgatgc gtgccgacca tgtataggca cggtcgaaaa
    25501 ttttgggcat cgattcttca ggaacggttt taatcatagc gtacccaccc aacacaaaaa
    25561 gtgtaataga cagcagcaat aatcccaatt tttttataga tattcctcca ataaacatca
    25621 taaggaatac gacaccaaaa agaagcatgg cagtagaaaa attttcaaaa agaatcaaag
    25681 gacatattgc agacagtaca atgagaatat accgaaaata cttcttttcg tctgctgctt
    25741 catttttgat gcgcgacata aaatccgcca ctacaataat gaccgaaagt tttgccagtt
    25801 ctgaaggctg aaattgcagt ccgccaattt caaaccaacg tgttgcgtcg tttgccgatt
    25861 gacccttgta ataaaccaga atcaggagta tgaacgacaa aaacagtccc aaataagcca
    25921 gcaatcgaat atatttgtat ggaatcagat ggacaagaaa tgccaaaata acgccaaaga
    25981 gcaaaaaacc ggcatggcgc aaaaggggca tggccgtgtt ggaagatttg aatgccagtg
    26041 tgctcgaagc actgtacatt tcaagaatgg aaaccaggca caacaaaatg aatacaatcc
    26101 aaattgttgc atttcctttt aaatattttt tcagaaaatt ttccatctgt tttttcgctt
    26161 aaacgcataa attattgata aatcacagat tccttacaca agttttaaat tgtcttcctc
    26221 tgtcttcata attctgaaat aaatcaaagc tggcacaaca tggcgatagc aaaaccgtat
    26281 ctccttcttc ggccattgca taagctgctt tcacggcatc ttccatcgag cggacatcca
    26341 cgattttatc tatttttcca tcgaaaaaac gatgtaaagg cgtgttgtct actcccaaaa
    26401 aaaccaatgc tttcactttt tctttcacca gactttcaat ttcagagtaa tcgttgcctt
    26461 tgtccgtccc gcccaaaatc agaataacgg gtgttttcat gctttccagc gcataccagc
    26521 atgaattgac attggtagct ttcgagtcgt tgatgaaagt tacgttttta actgtagcta
    26581 catattcgag gcgatgttcc acgccttgaa aatcctgtaa cgactggcga ataaattctt
    26641 tttttacatt cacagccgat gccgccaaac cggcagccat catattgtag gtgttgtgta
    26701 ttccttttaa cgacaattct tttaccggca taatcaatgg gttgttagaa gtttctatga
    26761 tcaggttgtt gttttcgata taagctttac aatgctcttt ctttttgagt gaaaagggat
    26821 attttgctgc gagtatagag cgttttgtta tttctgactg aataaccgga tcatcgtccc
    26881 aaaaaatgaa agcgtcttca gaggtttggt tacggatgat acggaatttt gaatcgacat
    26941 aattttgaaa attatattca taacggtcca aatggtcggg tgttatgttg agcaatattg
    27001 ctacatctgc tttaaaatca tacatcccgt ccaactgaaa actgctgatt tctaccacat
    27061 agtaatcgcg cgaacttgtt gctacctgat aggcaagact ttgtcctacg ttgcctgcca
    27121 gaccaacatt gtaacctgca tttttcaaaa tatgataagt tagcatggtt gtggttgttt
    27181 tcccattact gccggtaata caaatcattt tggcatttgt aaaccgccct gcaaattcaa
    27241 tttcggaaat aatgggaata ttcttctctt tcaaattttt gataattggt gctttatcgg
    27301 ggattcctgg actttttatt acttcatcgg cattcaaaat cagcgcttcg gtatgttgtt
    27361 tctcttccca ttctatttga tactggttga gcaactcttt gtagttttcc tttatttcgg
    27421 acaagtcgga aacaaacaca tcaaaacctt gctgttttgc caatacggca gcgcctgctc
    27481 cactttctcc cgctccaagt acaacgattc gtttcatatc ttttttgaac aaaattttct
    27541 gaaattttcc aagaatttat ctgattttta atgtaataat agttaatgca gccaaaatca
    27601 tgctcacaat ccagaatctg actacaattt ttggttcggg aactgcttgc aagggtcgtt
    27661 gaattaacgc ttcaattccc gcatttccgg gtttttgaaa atgatgatgc aaaggtgtca
    27721 ttttaaaaat tcttttgcca acaccgtacc ttttttttgt gtatttaaaa tagctcacct
    27781 ggcagattac cgacaggctt tcggccaaaa agacaccgca caaaatgggt aacaacaact
    27841 cttttctgat agcaatggca aaaacagcaa tgatacctcc caacgtcaaa ctccccgtgt
    27901 cgcccataaa aatttgtgca gggaaagaat tgtaccatag aaaaccaatg gtggcaccca
    27961 aaaaagcacc ggcaaagacc agcagttctc cggttccggg aatatacata atattcaggt
    28021 aaccggaata gttgatattg cccgacaagt aggcaagtac gccaagcgca cctcccataa
    28081 ttgccgacga accggctgaa agtccatcca atccatcagt caaatttgca ccatttgata
    28141 ctgccgttac aatgaaaata gcaaccagta caaacagaat ccatccatac ttttgtgcat
    28201 ttttccccgc attcttaaac gcatttgaat aatcgaggtc attgtttttc acaaagggaa
    28261 tggtggttcg ggtagctttg atatcttttt ttgaatatac tacatcttca actcgcaatt
    28321 ctcctctgat ttcggtgttt tcgcgcataa ccacgtcagg actgaaatac attaccaatc
    28381 caactatcag tcctaatcct acctgaccaa ctatcttcgc tttgccgcta agtccttcct
    28441 tgtttttcag aaatactttt atgtaatcgt caacaaatcc aattgctccc atccaaacag
    28501 tagtgatgag catcagaatg atataaatgt tcgtaaggtc ggcaaaaagt aaagtcggga
    28561 taagaatagc attgataata ataattccac ccatagtggg agttcctttt tttgaaagct
    28621 gcccttccaa tcccaaatcg cggattgttt cgccaatttg cttcttttgc aaaaaatcaa
    28681 tgattctccg tccaaaaagc gtggaaagca acaaggcaaa aataaaggac aaacccgttt
    28741 tgaacgaaat gtaattgaac attcctgctc cgggaatatc gtaaaacttg tccaaatagc
    28801 gaaataaatg atagaacatg tccgtttctt tttttgactt cgccttttct gaaaacagga
    28861 aagtcttggt tagtaatttg aatttttttt cttttatagc cgaataaaat cttttaaaaa
    28921 cattctttta ccacttctcg atcatcaaaa tgatgttttt cgcccttaat aatctggtag
    28981 tcttcgtgtc cttttcctgc aatcagcacc acatcgccgg gttgagccag cgagcaggcg
    29041 gtatgaatgg cctgacggcg gtcaggaatc acaaggcttg tttttctttc ttcgtcggtc
    29101 aatccggcaa tcatatcgtt cagtatgtcc atcggatctt caaaacgtgg attgtcggaa
    29161 gtcagaatgg ttttccaact gccggttacg gcttcttttg ccatcaaggg acgttttccc
    29221 ttgtctcgat ttcctccaca gccaactacc gtaattagtc ggcctttgtt ttgcaaaacc
    29281 ttatggatgg tgctgattac attgttcaaa gcatcgggag tatgtgcgta atcaacaata
    29341 acggtatatc cagacggagc atgcaacgtt tccaatcgcc ctgaaacagg atgaagcaaa
    29401 ctcagaattc tcagtacttc tgtttcttct tctcccatca gtaccgctgc tccgaatacg
    29461 gcggtcagat tgcttgcatt gaaccgtcct acaaatgaaa cgtgtacttg tttgccgttc
    29521 atttccaaaa gcattccttc aaaatcctgt tccagaatac gggttttaaa atcggccatc
    29581 gatctgagag aataagtata tttttttgct tttgtgtttt gcagcatgac cagcccgttc
    29641 ttatcatcta agttggttaa agcaaaagca ttttcgggca aatcgtcaaa aaattttttc
    29701 tttgctttca ggtaattttc aaaagtcaaa tggtaatcca gatgatcacg cgtaatattg
    29761 gtgaaaattc ctcctttaaa aagcaatccg gcaattctac gctgatcgat ggcgtgagaa
    29821 cttacttcca taaaggcata ttgacaacca gcttcaacca tttgagcgag cagatgattt
    29881 aactccaacg gatcgggagt ggtatgggtt gcttctacag gctcatccac aacataattt
    29941 cgaacagtcg acagcagtcc ggctttgtat ccgagctgac ggaacatctg gtaaagcaag
    30001 gtggcaatgg tggtttttcc gttggtgccg gtaacgccga ccaaagtcag tttttcagat
    30061 ggattgccgt accatgcaca agccaaaagc cccaatgctt ccaaactgtt ttcaacgcga
    30121 atatagacga ttccctgctg gatagtttca ggaaacgttt cgcatactat ggcagaggct
    30181 cctttctcaa tggctggaga aatgtaattg tgtccatcaa cattggtgcc acgagtagca
    30241 acaaaaatat tccctttatc cacttttctg gaatcggact gaacaccgga aatttccatc
    30301 tccgtatttc caataatttc agttgggaaa attttcttta aagcgacttt caaaatcata
    30361 actttttgtt tttaatttac tgaaaatgaa gagaaaggat ttgacccttc gttatttttg
    30421 tgccgggact gatactttgc gaaaccaatt ttccgcgacc acttatcatt acttttaagc
    30481 ctaatttttc agccacaaaa acagcatctt tcgctcccat tccttttaaa tcgggcatca
    30541 attgcctgta aacggttttt tttgaaattt ccaaaccatt atttttttca tcgggcaaca
    30601 cgttgacaaa acttcctgca gttcccgaaa taggtagtcg catttccgac gtaacttttt
    30661 ccaaatcgct gaaactgccc cataaagcat aaggaacttt tggtacattt cgcatggtat
    30721 ctggtacgaa atgttccggt tttaatgtgg atctcaaaaa catgattcgt tctgcgatat
    30781 ctttgaatac cgaaccactc attttgccgc ccgaaggata gccgattctc ggttcacgaa
    30841 tcacgacaat gcaagtatag agtggatgtt cggccggaaa atatccgcag aaagacactt
    30901 ggtgtgattt tcctcccgaa cgataaccga gcgcaccttt tgaaatttgt gccgttcccg
    30961 tttttcctgc aatgcgaacg tatttggaac gtacgttttt agccgttccc attcgccctt
    31021 ctacaacccc caacaacgtt tctcgaatgg tttgcaaaac agatggcttg caaatttgtt
    31081 cattgatgat ttcggtctga aaggttttta aaatttgtcc gtttttactc agcgctttta
    31141 caaaataagg ccgtatcagt tttccatcat tggcaattga attgtaaaaa gccaaagtat
    31201 atatgggtgg aatttgggtt tcatatccta tcgacatcca cggtaaagtg gttttcgacc
    31261 aatattttga tttgtcgagc ggatgacgga tatttggagc ggctgttccg ggaatttcca
    31321 aattcattgg ttcgttcact ttcattctgt acagtttatc tacatacgca gccggattgt
    31381 tgccgtaagc atttacaatc attcgggaaa ttccgatgtt agaagaagca tgaattgctt
    31441 cggctatggt gattttatga aaaccgcctc tgttgtaatt gtgatctttc ataaaagcat
    31501 tagcaaactg aaaaattccg tttccggtat ctattgaatc ggtaacttta actttaccgt
    31561 cgtcgagtgc agccatcaga gaaacgactt taaacgtaga gcccggctcc accatatcgg
    31621 aaacagcgcc gtttcgattt tcggaatagg ttccattttc atttttctgc atgtttacaa
    31681 tggctttaac ttctcccgat tgaacttcca ttacaataac gtaaccttcc tgcgcatcga
    31741 aagaccttaa actgtccaac agcgcttttt cggcaatatc ctgaatgtca acatcaatag
    31801 tggtaatcac atcaatcccg tcaatgggtt ccacttgtac cgtttcctgc catgtattgg
    31861 caactttctg gcgaatggct attccgggtg ttcctcttaa aagggagtcg aatttaagtt
    31921 caagaccgtt tttcccgcct ttggtttcgt cggcataaat atctccaatg gttcgggagg
    31981 cgagcgaccc gaaaggtttt actcttctaa attgttcttt tgttatcaaa ccgcttttgt
    32041 tttttcccaa acgaaacaac ggaaaagttt ttaattcctt taattgacta taagagattt
    32101 ttttcggata aatgcgaaaa gctcccaact ttttgttgta agcttccgta atgtcttttt
    32161 tgtattcggc ttgtgactta tctttaaaaa attttgacaa acaaagcgac aatgagtcaa
    32221 tattttcgtg aaacagttgt ccgttgtttt cttgcagcgc cggcacccgt aagtccatgt
    32281 aaatgtaata agtaggtatg gaactggcca tcaaacgtcc gtcggaagaa aaaatatttc
    32341 ctctgttggc tttcacttct atattggttt ttacattttt tgctgccaaa gccagccaat
    32401 tttcgcgctc aaaaaactga atggtaatta ttttgtaaat caccagcaga aaggcaacga
    32461 ctataaaaac atatacaatg ccaaagcgaa atacaatatt tttatgttgg tctgccatat
    32521 caattcttat tgtatcgaat cgtccaatac taccaaagga gaaaccggct cgtgcagttc
    32581 gatgcctctt tctttcaaca ttttttccac gtttgactga cggctgacct gcatcagttc
    32641 tgccgagatg ctgagcgatt catatttttt gtcctggact tccttcttca atttgttttg
    32701 gtaggtcaat tccgattcgt attcgtagcg gttgccaata taaaatataa aaagcaccac
    32761 aatcataatc aaaagaccta tttgcttgat aaaaaatctt ttggtgaaaa tatttccgtt
    32821 gaggatatcc cgaaatgagc tcgacttgag ttcggaaaaa tcctcactgc cctttatgtt
    32881 ctctatgatt ttactgaacc aactcattcg ttatttcaaa gtttaactct ttcattaaat
    32941 gttcaaaaaa tcaatcaatt agttttgtca atcaattcgg caatgcgtaa tttggcgctt
    33001 ctggctctcg gatttttttc aatttccgaa agctgtggcg taatagcctt gccgcttata
    33061 ggcttgaagg gagcaatgat attcccaaaa aaatctttgt ttaaaatacc ttcaaagttc
    33121 cctgttttca taaaattttt caccatgcgg tcttcgagcg aatgataagt gagcacggcc
    33181 aatcttcctc cgggtttcag cacatcgggg gtttgctgca gaaattcctg caaagcttcc
    33241 aattcgtgat ttacttcaat gcgcaaagcc tgaaaaacgc aagcaagtcc ttttttttct
    33301 ttctgacggg ggaaaaagcg tttcatcatt tctacaaacg gaagaatttt ttgaaaaggt
    33361 tccctgtctc tcgcttttac tatggttgct gcaatttttc gtgcattgga caattctccg
    33421 taatgataaa aaacatcggc caactgttct tcagtgtagg tgttcagcac ggtagcagcc
    33481 gtcagttccg tttttctgtt catgcgcata tccaattctc catcaagccg gaacgaaaac
    33541 ccccgttcgc tttcgtcaaa gtgatgcgaa gaaacgccca aatcggctaa aatgccatct
    33601 accagagaaa taccatgata acgtagaaag ttttttaaat aacaaaaatt actttgaacg
    33661 aacaaaaatc tgtcatcctc aaaaatattt tggcaggcgt cctggtcctg atcaaaagcg
    33721 ataagttttc cctttttgct cagtaaatcg agaataccac gtgaatgtcc tccgccgccg
    33781 aaggttacat ccacatagat accgtccgga ttgagtacca atccttgcag cgtttccttc
    33841 agcatgacgg gtatgtgata ttccttttca gacatgaaat tataattagt tattttgaca
    33901 ttttttcctt cagcaaagcc gcaaaatttt cggctgacaa ttgcatttgt tcgtaccgac
    33961 ttttgctcca aagcgcaaaa cggtcaatca tgcccacaaa aagcacttct gcgttttcaa
    34021 cgccaatggt ctgcaaatgt tttttcgaaa tcaatacacg tccctgcgca tcaatatcca
    34081 gccattccgc atctgccaca aactgcatca gtaaaagctg atcggaagga ttccattcgt
    34141 ccaatttcga ttttaagttt tctacctttt gattccagat actttccgga tagaacacaa
    34201 tacattcatt ccctgcgtct ttgcgcatga tgatttgttc acggtcgtct acgcccaaga
    34261 tttttcgata agcggaagga atgaatatgc gaccttttac gtcggctttg gcttcatatt
    34321 ttccaataaa tgtagacatt gcagtagcta tattttttca actgtaaaaa tatggaatta
    34381 ttttcaattc cccacattca cccacaaaaa agttataaac aattttttta aattatttta
    34441 gcttgttttc aaccgctttt caacaattta tcaacaacaa gaaattagac aattattttg
    34501 taaacatcag aaaataaaga ttttatattt tattcattca tttttatatg gtaagtggtg
    34561 aaaaatattt gtgaatttca aaaatgttga taacttttca acgaaaaatg caatgataaa
    34621 aaacacaaaa atttcaaatt attgtaactt tgcaatagaa caacaccatt ttattttcat
    34681 gaatagtaaa ccggattttt taattgatat agaaaaagtt gtcaaggaaa aagcgcctaa
    34741 tacttttatc ccttcttttt tggtgagtta tttgcgtaaa attgttcacg aaaaggaaat
    34801 caatgagttg ctgaccactt attcgcacca aaagaatgta gattttctcg aaagcgcagt
    34861 aaaatatttt caggtcaatg tttccattaa aaacagagaa aatttgcctc atgacgataa
    34921 actttatatt tttgccggaa atcatccgtt gggaggaatg gatgccataa tgaccggtag
    34981 cttgttgggc agggaatatg atgggaaaat tcgatttatg gccaacgata tgttgatgtt
    35041 tatcgagccc ttgaaggact tgtttatccc ggtcaacaaa gtcggttcac aggggaaaaa
    35101 ccatgtccgc gaattgaacg attttttcag ttccgattgt catctgatta cttatccggc
    35161 cggaaaatgc agcagaaaat ccaacggaaa aattcaggat gaagcatgga acaagaattt
    35221 tgttgtcaaa gctattcaac atcagcggga tattgtcccg atttattttg acggaaaaaa
    35281 ttcaaatttc ttttacaacc tggccaatct gagaaaattt tttcatataa agttcaatat
    35341 cgaaatgctc tatctgtccg acgaaatgtt caaacaaaaa gggaaaagtt tttcgctggt
    35401 tatcggcgag cctattcgtt ggcaaacttt tgaccgttca aaaacttacg gcaaatgggc
    35461 gcaatgggtc aaagagaaag tttatgcctt gtccgaaaaa actttctaat aaaattacga
    35521 ttattcctaa aagggcatta ataaaaaaaa gacagagcaa aaaaagccct gccttttgaa
    35581 aaaatagata taagttttaa ttgttttctt ctactacttc gccttcttca acatcaatta
    35641 agcttttgcg tgcaaaaact ttcttttcgt attcttcacg ggaatagaca acaatttttt
    35701 caaagtcgcg ctgtccagta cctgcaggga ttaaatgacc gcaaataacg ttttctttca
    35761 agccttccag cttgtccact ttggcattaa tggcagcttc gttcagcact tttgttgttt
    35821 cttggaaaga agcagccgac ataaagctgt tggtttgaag tgcagcacga gtaattccct
    35881 gcaaaatctg attggatgtt gccggaatgg catcacgagc ctgaaccgga cgcaagtctt
    35941 tccgtttcaa catactgttt tcatccctca actttctggg cgtaataatt tggccgggtt
    36001 tcaaagtagg agaatctccc gcatccacaa taactttctt tccccaaatt ctgtcgtttt
    36061 cttccataaa atcatttttg tcaactacct gtttttcgag gaaacgggta tctcccggtt
    36121 caatgatttc aaccttgcgc atcatctgac gaacaataat ttcaaaatgt ttgtcgttaa
    36181 ttttcacccc ttgcaggcgg taaacatcct gaacttcgtt gacaatgtaa tcctgaacgg
    36241 cagtaggtcc cttaatctgc aaaatatcgg ttggcgtaat tgagccatca gaaagaggcg
    36301 tacctgcacg aacaaaatcg ttttcctgta ccagaatttg ttttgaaaga ggaatcagat
    36361 attttttaac ttcaccggtt ttggaagtga tgcttaactc acgatttcca cgtttgattt
    36421 ttccgaaagt aacttctcca tcaatttcag ccacaatggc aggattagaa ggatttcgag
    36481 cctcaaacaa ttccgtaact cgaggaagac caccggtaat atctccggct ttcccaacag
    36541 cacgaggaat ttttaccaga atctgaccgg tttttatata atcaccgttt tcaaacacaa
    36601 tgtgagctcc aaccggtaaa ttataagttt tcaagatatt gccatcctta tccagaatat
    36661 gagctgtagg aactttgttt cggtctttcg attcgatgat aattttctct cgaagtcccg
    36721 tagcttcatc ggtttcaacc ttaaaagtaa cgttttcgac tacatcttca aattcgatct
    36781 ttccttcgtt ttctgcaatt ataacagcat taaaaggatc ccattcgcaa ataagctgac
    36841 ctttagtaca aatatctcct tccttcacat acaatttcga tccataaggg atattcatgg
    36901 tagtaagcac aatacccgta ttaggatcca caacgcgcat ttcggccaaa cgacttacta
    36961 caacgcgata gctttgatca tcagagggct gaacggtacg aagttcgtca aattcgagac
    37021 gtccatcata acgaagtaca atttttgatt cggcagctat atttgaagca atacctccaa
    37081 catggaaggt acgaagtgtc agctgagttc ccggctctcc aattgactgg gcagcaatga
    37141 cacctacagc ttctccaatg tttaccattc ttcctgttgc caaatttcgt ccgtaacatt
    37201 ttgcacagac tccttttttg gattcgcagg tcaatacgga acgaatttca acttgttcga
    37261 taggagaatc ctgaattttt ttggccagtg attctgtaat ttcttctccc gaagatacga
    37321 ttaattctcc ggtaagcgga tgataaatat cgtgaacgga aacacgtccc aaaattcttt
    37381 catacaaaga agaaattact tcttcattat ttttcatttc ggtagcaatc aagcccctca
    37441 atgttccgca atcttcctca gtaatggtta catcttgagc aacatcgacc agacgtcgag
    37501 tcagataccc tgcatcagcc gttttaagtg ccgtatcggc caaaccctta cgggcaccat
    37561 gcgtggagat aaagtattcc aacaccgaaa gtccttcctt aaagttggaa agaatcgggt
    37621 tctcaataat ttgtcctcct tccgataccg atttctgagg ttttgccatc aaaccacgca
    37681 ttcccgacaa ctgacgaatc tgttcctttg aaccgcgcgc tcccgaatca agcatcatat
    37741 acacagaatt gaatccatca ttatcgctcg acaattcctt catcagcaca tcggacaatt
    37801 tggaatttac gtgcgtccaa gtgtcgataa tctgattata acgttcgtta taggttatga
    37861 atcccatatt gtagttattc agaatttctt ctacttccct gttgccttct tcaataagtt
    37921 tttccttatc ttttggaata atcacatcat ccaaattgaa ggaaagtccg cctctaaagg
    37981 ccatattgta gcccaattcc ttgatatcgt ccaaaaaatg agccgtacgc gccattccac
    38041 aagtttctat aaccctgccg ataatgtctc tcaaggattt ttttgaaagc aattcgttga
    38101 tatagccaac ttcttccgga acatatttat tgaaaataat tcgtccaaca gtggtttcaa
    38161 tcagtttttc caccgaattc ccgttttcgt ccaaatcttt ggtacgaact tttacgcgag
    38221 catgaatatc tactttatgt tcattataag caatttctgc ttcttcggga gaataaaata
    38281 tcaaaccttc acccaaagca tttggtcgag gttttgtaat ataatacaat cccaatacca
    38341 tatcctgtga aggaacagta ataggagctc cgttggccgg attcaaaata ttgtgagaag
    38401 ccagcatcaa caattgagct tccaaaacag cctcattgct caacggtaaa tgcacggcca
    38461 tttggtctcc atcaaagtcg gcattgaaag ccgtacaagc taaaggatgc aattgaatgg
    38521 ccatcccttc gataagtttg ggttggaatg cctgaattcc cagccggtgc aatgttggag
    38581 ctcggttaag taaaaccgga tgtcctttca ttacatattc cagaatatcc cagataacag
    38641 ggtccttgcg atcaataatt ttttttgccg attttacagt ttttactatt cccctttcaa
    38701 taagttttcg gataataaac ggtttgtaaa gctcggcagc catatctttg ggcaatccac
    38761 attcatgcat tttcaattcc gggcctacca caataactga acgagccgaa tagtcaacac
    38821 gttttcccag caagttctga cgaaaacgtc cctgtttccc tttcaatgaa tcggaaagtg
    38881 attttaaagg acggttggct tcggtcttca cggcacttgc tttacgcgaa ttgtcgaaca
    38941 acgaatccac agcttcctgc aacatacgtt tttcattgcg taaaattact tccggtgcct
    39001 tgatttcaat cagacgtttc aggcgattat tgcgaataat tactcgacga taaagatcat
    39061 ttaagtctga agtggcaaaa cgaccgccat ccaacggaac caacggacgc aattcaggag
    39121 gaataaccgg aacaatcttc acaatcatcc actcgggacg atttctattt ttagaagctc
    39181 tgaaagcttc caccacctgc aaacgtttca aagcttcggc tttacgctgc tgagaagtat
    39241 cggtgttggc cttatgacgc aattcatagg aaagcgcatc caaatctatt ctgctcaaca
    39301 gatcgtaaat ggcttctgca cccattttag caataaactt attgggatcg gtgtcttcca
    39361 gttgttgatt atcttttggc agcgaatcca aaatatccaa atattcctct tcggtgagta
    39421 gttctccggt ggcaatattg tctccatttt caagaatgcc tccctgaata atcacatatt
    39481 tttcgtaata aataatcgat tccagttttt tgctcgacat tcccagcaga tagccaattt
    39541 tactcggtaa agtgcggaaa taccatatat gggcaacagg aacaaccagt tggatatgtc
    39601 ccattctttc acggcgaact tttttctctg taacttctac cccgcaacga tcacaaacaa
    39661 ttcccttgta tcgaatacgc ttgtattttc cgcaatgaca ttcatagtcc tttacagggc
    39721 caaaaatgcg ttcgcaaaac aaaccgtctc tttcaggctt gtaagttctg tagttgattg
    39781 tttcgggttt gagtacttca ccatgagaca atttcaaaat ttcttcgggt gaagctaatc
    39841 cgatagaaat tttcgagaaa ttgctcttta ttttattatc agttttaaaa gccatatttt
    39901 ttatttcaat ttaattaaat tgacaatttt aaattaattt tggataggaa tgaacgttat
    39961 gataaattca cgttccctat tccaaattaa tgctcaagca cagaccgcgt aattcgtgaa
    40021 gtaatacatt caatgattcc gggattccgg gagtaggcat tggatctccc ttcacgattg
    40081 cttcataagt ccgggcacga ccattgacat catccgactt aacggtaaga atttcacgca
    40141 ggatattggc agccccgaat gcttcgagtg cccatacttc catttctcca aatcgctgtc
    40201 cgccgaactg ggcttttcct cccaaaggtt gctgcgtaat cagagaataa ggtccaatag
    40261 aacgagcgtg catcttatct tccaccatgt gtcccagttt catcatgtaa ataacgccta
    40321 ctgtggcagg ttggtcgaag cgttcgccgg ttccgccatc gtacaaataa gttttacctg
    40381 cacggggcaa ccctgctttg tctgtccaag catttaagtc gttgagcgtg gcaccatcaa
    40441 aaattggcgt tgcaaaacga actcccaatt ctttacctgc ccatcccaaa acactttcaa
    40501 aaatctgacc caaattcata cgcgaaggta cacccaacgg gttgagcaca atatcaacag
    40561 gcgttccatc ttcgaggaaa ggcatgtctt cttcgcgtac aatccgcgaa acaatacctt
    40621 tattcccgtg acgacctgcc attttatccc caacacgaat tttacgtttt ttggctatat
    40681 aaactttggc catttgcata atgccacttg gcaaatcatc tccaatggta atgttgaatt
    40741 tttgcctttt cagctgcgca tccaattctt tgaattttct taagaaattg gtaatcaaac
    40801 tgttcaccat gtcatttgta tgagcgtcgt ttgtccattt tccaagctga accgtagtat
    40861 aatctatttc gcgcaaccgc tcaacagtaa atttactgtt acgtggtata atatcggtac
    40921 ccaaaaagtc cttcacaccg gttgaagtct tgttggtcaa aattttcatc aacttttcta
    40981 tcaaagtttc acgcaacgca ttggcttgtg tttcaaactc ttcgtccaat tttggaagaa
    41041 ctgccttgtc agctaatttt gatttacgat ttttctgagc tttggagaat aatctttttc
    41101 caatgactac ccccgacaac gatggtgtgg ctttcagaga agcatctttt acatcgcctg
    41161 ccttatcgcc gaatattgca cgaagcaact tttcttcggg agaaggatcg gattctcctt
    41221 ttggtgttat ttttccgata attatatcac cgggttctac gcgtgcacca actcgaataa
    41281 tcccgttttc gtcaagattt ctggtagcat cttcactcac attgggtatg tcggaagtaa
    41341 attcctcagt gccacgcttg gtttctctaa cgtccaacaa ttgttccact acgtgaatgg
    41401 aagtaaagac atcttctcga accacctttt cgtttatcac aatagcatcc tcgaaattgt
    41461 atcctttcca cggcataaaa gcaactttca agtttttgcc caaagccaac tctccacttt
    41521 cgctgccata gccttcggtg ataatcattc ctgcgtgtac acggtctccc ctgcgaacaa
    41581 ttggacgaag gtcaatggtg gtattctgat ttgtcttttg atatttagga agtttatatt
    41641 ccttaacagc agaatcgaaa cttacaaatt cttcttcttc agtgcggtca tagcgaattt
    41701 taatgcaatt tgcatcaaca tattcaacta caccatctcc ctcagcagtt atttgcgtgc
    41761 gcgaatcatg aatcactctc ccttctatgc cggtggccac aatcggggct tcacaccgta
    41821 gcaacggaac tgcctgcctc atcatgttcg aacccattaa ggcacgattg gcatcatcat
    41881 gttccagaaa aggaattaat ccggctgcaa tagacgcaat ttgcgaagga gaaacatcca
    41941 tcaaatgaac ttccattggg tcaactaccg gaaaatcagc gccaagacgt gctttcactc
    42001 tcgaacgaat aaattttccg ttttcatcaa gaggagcatt cccttgtgct acaaccaaat
    42061 cttcttcttc ttcagcagta tagtaaccaa ttccgttttc actcaaatcg actactccat
    42121 tattaacttt acggtaagga gtttctataa aaccgagctc attgattttt gcgtacacac
    42181 acaaagatga tataagaccg atatttggac cttcaggagt ttctattgga cacagacgac
    42241 cgtaatgggt ataatgaacg tcacgcactt caaaaccggc tctttcgcga gacaaaccgc
    42301 cgggccccaa agccgacatc cttcgcttgt gggtaatttc agcaagcgga ttctgctgat
    42361 ccatgaattg cgataaagca ttggttccaa agaatgaatt aataaccgat gaaatactct
    42421 tggcattgat cagatcgccg ggagtaaaaa cttcattatc gcgaacattc attcgttccc
    42481 gaatggtacg tgccatacgt gccaatccaa cgctaaactg attgtataat tgttctccaa
    42541 ctgttctgac tcttctgttg ctcaggtgat cgatatcgtc cacttcagcc ttagagttca
    42601 ccaaatcgat cagatatttt ataatttcaa taatatcttc tttggtgagc actttcacct
    42661 ccatacttgt agtgaggttc agcttttgat tgatacggaa acgtccaact tcacccaaat
    42721 cgtatctttt ttccgaaaag aacagattgt tgattacttc acgggcagta gcatcatctg
    42781 gtggttcagc atttctcaat tgcctgtaaa tataaagtac agcttctttt tccgagttgc
    42841 ttggatcctt ttgcagcgta ttgtaaataa tgctgtaatc gttctgattg gcttcttctt
    42901 tatgtatcag tatggtttga gccccggatt ggagaatcag atcaatgtgc tcgttttcta
    42961 aaacggtttc acgatcgata atgatttcat tacgttcaat ggttactact tcaccggtat
    43021 cttcatccac gaaatcttcc aaccatgttt tcagcacacg ggcagcaagt ttactgccca
    43081 cagccttttt cagattggat ttattgactt tgatttcttc agccagatta aaaatttcca
    43141 aaatatcctt atcactttca aacccaatgg ctctgagcaa cgtggttacg ggcaattttt
    43201 tctttctgtc aatataagca tacatgacat tgttgatgtc ggtagcgaat tctatccacg
    43261 agccacgaaa tggaataatt ctggctgaat acaattttgt tccgttggca tgaatacttt
    43321 gtccaaaaaa tacccccggg gaacggtgta attgagaaac aataaccctt tcggctccat
    43381 tgataacaaa agtacctctg ggagtcatat aaggtattgg acccaagaaa acatcctgaa
    43441 taacggtatc gaaatcctca tgttcaggat cggtgcaata aagtttaagt tttgccttta
    43501 aaggcacact gtaagttaaa cctcgctcaa tgcattcttc gatagaatag cgcggtggat
    43561 ctatgtaata atctataaat tcgagtacaa aattatttcg agtatcagcg atggggaaat
    43621 tctcggtaaa cactttgtat aaaccttccg attttcgttt gtcggaggaa gtatccattt
    43681 ggaaaaaatc ctgaaaagat ttcacttgta cttccagaaa atcaggatac tccaactgat
    43741 tttttatgga agcaaaattt attctttgat tgttagtaga tgaagacatt ttcaaagagg
    43801 ttattggggt tcaatgaact cactatattg tatagacaaa aaggtttaga acctcccgtt
    43861 gcgaaaggtt ctaaaccaag ttactgatat tcagctgaca ttatttaatt tcgacttcag
    43921 ctccgccttc aaccaactga ttttttaagg cttcagcttc atctttcgaa attccttcct
    43981 ttataacaga aggagcagcg tcaactaaat ctttggcatc tttaagtccc aaaccggtaa
    44041 gttcttttac taattttaca attgccaatt tgttggcacc cggcgatttc aatactacat
    44101 caaaagctgt tttttcttca actgcctctg ctgctgcagc tgacgggcct gcagcaacag
    44161 caacagctgc tgcagcgggt tcgataccat aatcattttt taaaatctgt gctaactcgt
    44221 tcacttcttt tactgtcaag ttaaccaatt gttctgcaaa agctttcaaa tctgccattt
    44281 tattctattt tttgatttgt ttttaattta aaaaaattat ttttcagaaa gtgtttgtaa
    44341 aacaccatga atagtattag ctcctgattg tagagctgaa acgacatttt tagccggaga
    44401 ttgcaacaaa gcaatcaaat cggcaatcag ttcattcttg ctcttcacat tgacaagtgt
    44461 atcgagttga ttttcgccaa tgtaaaaact ttcttcaaca taagcagcct ttaaaacagg
    44521 ttttttgttc ttggcactga aatttttaat cagttttgcc ggaacattag ccatatcgga
    44581 aagcatgaga gcagtttctc cctttaaagt tggatacagt tcactgaaat caaccgatga
    44641 gcgatccagt gcttttttca acaaagtatt tttaaccact accagtttca ccttttcctt
    44701 gtagcaaagt tttctcaact cactggtttg agcggcattt aaaccgccta tgtcagtaag
    44761 gtaaaaatga ggatactcgc tcagaaccga ttcaatctga tcaatgattg tgcttttatc
    44821 ttccttcttc ataagtttaa tctttaaatt tcaattgatt tgggatctac ttttataccc
    44881 ggactcatcg tactagaaag ataaatgctt ttaatgtaag ttcctttggc tgtagaaggt
    44941 tttagtttta tcagcgtaga aataaattcc tgtgcatttt cggcgatttg ttctgccgta
    45001 aaggaaactt ttccgatgga agcatgaaca attccgaact tgtcgacttt gaaatctatt
    45061 tttccctgtt taacttcctt aatagccttg gcaacatcat tcgttactgt accgcttttg
    45121 gggtttggca ttaaaccgcg gggcccaagt attcttccaa gagctcccaa tttccccatt
    45181 acagcaggtg tagtaatgat gacatcaaca tctgtccagc cacctttaat tttttcgatg
    45241 tattcgtcca gtccaacgta atcggcgccg gcttctttag cagcagcttc caattcagga
    45301 gggcacaaag caagtacttt cacctgtttc cctgtcccat tgggaagtga aacgactcca
    45361 cgtaccattt gattggcctt acggggatca acacctaaac gcacatcgat atccaccgaa
    45421 gcatcaaatt tagtagtagt aacttctttt actaactttg cagcttcaat gagtggatat
    45481 gcctttccag cttcaatttt ttctaaagcg tattttcgat tttttgttaa tgtactcatt
    45541 gtaattgaag tgttaaattg ttatttacct ggaaaagtac ccttcacggt aatacccata
    45601 cttcttgctg tcccagctac cattctcatg gctgattcca attcgaaaca gttcatgtca
    45661 accattttat cctgtgcaat ttccttcacc tgatcccaag aaatttcggc aaccttttta
    45721 cggttgggtt cgggagatcc acttttgagt ttggtaagtt cgagcaattg aatggctacc
    45781 ggaggcgttt taacaacaaa atcgaaagat ttgtccgtat aataggtaat gacaacgggt
    45841 aagattttac cggctttgtc ctgagtcctg gcattaaatt gtttacagaa ctccataatg
    45901 ttgatccctt tagaacctaa agcaggtcct acgggaggag atggatttgc agcacctcct
    45961 tttatctgca atttaattac tccagcaatc tcttttgcca taattgtttt gatttttgat
    46021 tgttatcgga ataatcgact tgtaaccgag ccaactattc cttttctact tgagtaaacg
    46081 ctaattcgag cggagttttc cgaccgaata tcataaccat aactttgagt tttttctttt
    46141 cggtgtttac ctcttcaatc gttccactaa atcccgaaaa aggtccgaag atgactttta
    46201 cattttctcc taccatgaaa ggagttatca tttcttcttc actttcctgc aattcatcca
    46261 ttgtccctaa aattcggtta atttcggaag tacggatagg cgtgggaatg ttgttttttt
    46321 cgcctaaaaa ccccaaaaca ttgggtgttt cgcgaacacg atgaagagct tcacccataa
    46381 gatttgcttc tatcaaaacg taacccggca tgcaattcct ttctttagca atttttttgc
    46441 cgttacgaat ctgaaaaact ttttcagtag ggatcaaaac ctgacgtaca tactgtccaa
    46501 ggtcagaatt tttgatttct gtttctatat attcctttac cttgttttct ttcccactaa
    46561 tggcacggag tacataccat ttaaaatttg tcgtatcaga cactttctcg acctccactg
    46621 attaaagatt aaaagatttt ttcgtaaatg aacgtcatga tagattcaaa acttaaatcc
    46681 ataacccaga caatcaacgc aaggatgatg gaagcaatca atacaacaat tgcgctgttg
    46741 gtaagttccg agagcgaagg ccaagaaact ttatgaacca gttctgaata gcactctttg
    46801 atatagttaa ttattttcat gttttttgta caaattggca cgggcagaga gactcgaact
    46861 cccgacacct ggttttggag accagtgctc taccaaactg agctacgccc gtaggtaatt
    46921 tttcctttta cagactttga tgatttgaaa gcctgtaaaa ggaaaagagc ttaaaaagat
    46981 taattcatgt ttatttttta gtgatgtcca aaaaagtgaa cgacatccgt ctgtcaaatt
    47041 aatccaaaat ttctgtaatc tgaccagaac ctaccgtacg tccgccttca cgaatagcaa
    47101 aacgcaaacc tacgctgcaa gctaccggat aaatcagttc tacagtaaat tcgatattgt
    47161 cgccgggcat taccatttcc actccttccg gaagagtaat ttcaccggta acgtccattg
    47221 tacgaatata gaactgagga cgatagtgat tgtggaaagg agtatgacgt ccaccttcct
    47281 ctttcttcag aatataaacg gcagctttga atttcttgtg aggtgtaacg gctcccggat
    47341 gggtaataac catacctcgt ttgatttcat ccttatcgat acctctcaga agtaaaccta
    47401 cattatcgcc ggcctgtcct tgatccaaaa tcttacggaa catttcaaca cccgttacaa
    47461 cggttttctt ggcttcgtgt cccaatccga taatctgaac ttcgtcgcca acttttacaa
    47521 ttccagtttc aattctgcct gtagcaacag ttccacgacc tgtaatagag aatacgtctt
    47581 caacaggcat caagaatggt ttgtcaacat cacgttgagg taattcaatc caattgtcaa
    47641 cagcatccat caactcaaca attttagctt cccatttagg gtcaccatcc aatgcgccca
    47701 aagctgaacc acgaataacc ggagtattgt cgccatcga
//
LOCUS       DAOSIB010000005        47264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_895_length_47264_cov_11.766782, whole genome shotgun sequence.
ACCESSION   DAOSIB010000005 DAOSIB010000000
VERSION     DAOSIB010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 47264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47264
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_895_length_47264_cov_11.766782"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1776
                     /locus_tag="PLD44_01060"
     CDS             169..1776
                     /locus_tag="PLD44_01060"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOF97960.1"
                     /translation="MKKSQFNILQTFALLIISIIMSSCEDFLSSIRNENSQSIESLLY
                     NINDIDVCINGAYGAFCSNSYYNNVVLTQTLGSDEITNTKNPSILPDFLSISAYNYHF
                     SYQCNTDNYQAGSVLMWGSFVTNNSNVVIKSIENKLPNLRNFNDTLNAERLLGEAYLL
                     RACVEYYNNLYVGRQYHPSTLQLPASLYRKRAILGFEDIPEPRKTVEQVHQYIIEDLQ
                     NARKLLPQYYEKTIHPVAYQYRCKRDVAIAMLAKAYFQQNQFDSALVYINQLLGNQTG
                     ASNKYPLQTTNNYNQLFRIVDKTNYQANSKSEIIMGFHGNSAFRPTSTQEWSMFHWSA
                     FQNLQNSDLTNLRFRIVMDSALIKSFLKGDTLNDVRFKNMIYITKNIGKESPAGQWTS
                     LKHAYPTSNVVWLRAAEFHLMRAEIYLHKDNLSSSINELNIVRKRAGLDNYTFAGKTQ
                     LFQAIIDERFREMHFEGIRRSDNIRLASLSDSNAASYLPMPYKLGNIPLGNRALLITD
                     SILHWNDERLYCLIPENEYIYNPALKR"
     gene            1843..5226
                     /locus_tag="PLD44_01065"
     CDS             1843..5226
                     /locus_tag="PLD44_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOF97961.1"
                     /translation="MSKIELRRKQNIFNRLLLIVIIICLNLASAKAWAQKHDEKISLN
                     FENADFKQIMREIELKWDVVFVYNTSDLNEFGIKSLQTNNLDLYETLNTLFAGTDITY
                     TIKDVYVFLRAKKDTNNLTLKESKSIMGVIVDELQQQLPGVYITDKQGNILGISNEDG
                     SFNIYSIPKDSSLYFFMHGFKRETLKLLNNNNIYKVTMRSNNLLLSELLVIGYGSMQK
                     RETTGAIQSLPKFAIKNNVGGNIGNSFEGNVAGLWASNQNYRIRGVSSINSSADPLVV
                     IDGIPQTIGLNDINPNDIASVEFLKDAASAAIFGSRASNGVIVISTKSGDLNSKQKTI
                     AEYRTSINYVTNKPKFIKGKELISLLDAAYYNRYPENKTLEDNNPIKYFPFSPDYANF
                     LGFNRDWLNTYLLNNPLGTDWESALTQAAISQDVRLSFQGGQKSNSYYISLSHKSDNE
                     LVDNKNKKRSTLVLKNDFKISERINTGFNINFTLNTSKNSTFDKLESLFSRSSLLPIY
                     APDGSGKYFDARNINDKKGSNPLYRMSETWDDNINFNGLLLGYFDIKLMQKFAFRSEL
                     SMTPGTSRYRYFQSKDFYREDEAIDPSKSGIILYSRTLSYGFNVNNLLNYNTKINNRH
                     VIKVMLGNNIQSFNSDFNVARFEGFPTNYFQLTNANTEKVLTRQSAGMDGYRFVSFFN
                     RTQYALDEKYFFELNARADGTSRFINDNRWGFFPGLGMSWMLSEENFFSQIKSIDYLK
                     FRTSYGFVGNAEVGNFPAESRVLNWAEYAGSPGFVLDRIANLDVRWEKQLQLNVGFNI
                     SALKNKISASLDWFLKRNSDLLLNYNIGKFQGYFNTEVPINSGNLNNNGFDLDLKTTN
                     YDGLFKWKTEFNISMFNTKISKLSTQQNYIENGINRAYVGYPLGLYYLPLWAGVNPET
                     GHEMIYEVIGPETNKTKTGKLLDAEMLTSVEYRNQSVLITDKTPYPKLYGGVSNFFAY
                     KKFELNIHFSFQLGNWIYHNGMRQNSYVNTYDINNKMVSLQNYWTPENKNSNTPLLYN
                     SQMANRNSTRYLMNASYLRLRNLSMNYTFDSKNTKKFGLGKIIAQINCQNLITLSKFS
                     NGSPELSLQNAGAEANISLGNIGLNYGVATLSAGIKLEF"
     gene            complement(6005..7051)
                     /gene="ilvC"
                     /locus_tag="PLD44_01070"
     CDS             complement(6005..7051)
                     /gene="ilvC"
                     /locus_tag="PLD44_01070"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOF97962.1"
                     /translation="MAVINFGGVDENVITREEFPLSKAREVLKDEVIAVIGYGVQGPG
                     QSLNLRDNGFNVIVGQRKGGKTWEKAVADGWVPGETLFDIEEAAERGTIIQYLLSDAA
                     QIEVWPRIKPYLTPGKALYFSHGFGITFKDRTGIIPPPDIDVIMVAPKGSGTSLRRLF
                     LEGRGLNSSYAIFQDATGKAFDRVIALGIGVGSGYLFETTFEREVYSDLTGERGTLMG
                     AIQGLLSAQYEVLRAHGHSPSEAFNETVEELTQSLMPLFAENGMDWMYANCSTTAQRG
                     ALDWMGPFHDATKPVFEKLYNEVASGREAQRSIDSNSRPDYRERLEEELKALRESEMW
                     RTGAVVRKLRPENN"
     gene            complement(7124..7855)
                     /locus_tag="PLD44_01075"
     CDS             complement(7124..7855)
                     /locus_tag="PLD44_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOF97963.1"
                     /translation="MEKEFSYIFKIAPSNVDFQGKLTLASLIELLMEAAGRHADGNGF
                     GMRQLNEFNASWVVLRIALEINYYPEQYDEIKISTWIEKIQPIKTIRNFCIENAQGEI
                     IASATSEWIMMDLTQRKPKDLLSLEGFEEFATGKEISAGKLEKLPPVKGELVDKITVK
                     YGDIDINGHVNSIRYVQWLCNCFNLSDYREKQIERFEIHYISELHENEIIKIFREEKK
                     KNDFYFEIDSDKKTTCKARISFSEK"
     gene            complement(7897..8469)
                     /gene="ilvN"
                     /locus_tag="PLD44_01080"
     CDS             complement(7897..8469)
                     /gene="ilvN"
                     /locus_tag="PLD44_01080"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOF97964.1"
                     /translation="MMEKTLYTVIIFSENTVGVLNQITVIFTRRGLNIETLSVSPSAI
                     AGIHKFTITVITTQDVIEKVVQQIDKRVDILKAYYNTDEDLVHQEIALYKVATDKLLE
                     LGSIEKMIRDYQIHVLELTENCVVFEKTGHYQETQKLFEELSSTIGVLQFIRSGRIAI
                     TKSKVERLSDMLEKMSKLEQKVNATTETQE"
     gene            complement(8533..10218)
                     /gene="ilvB"
                     /locus_tag="PLD44_01085"
     CDS             complement(8533..10218)
                     /gene="ilvB"
                     /locus_tag="PLD44_01085"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOF97965.1"
                     /translation="MKMTGAKALIESLLQEGVETIFGYPGGAILPVFDALYDYDRKVN
                     HILTRHEQGAAHAAEGYARVSGKVGVCLITSGPAATNAITGISDAMLDSVPLLVITGQ
                     VGASLLGTDAFQEIDVIGITQPITKWSYQIRRAEDVAWAVARAFYIASTGRPGPVVLD
                     FTKNAQSEEVDFQYKPCKFIRSYVPVPEVNFSQIEKAAELINAAEKPFAVVGHGVILG
                     QAEKDLMEFLEKADIPAAWTLLGASAMPSDFRLNKGFVGMHGNVAPNMKTNECDVLIA
                     IGMRFDDRVTGDLKTYASQAKIIHIDIDAAELNKNLKVEVPVLGSVKDVLPALTKLVK
                     PAKHTEWIDSFDIFEKTEYEKVIEREVFPKSGKITMGEVIHKVSEATQHNAICVTDVG
                     QNQMASARYFKFSQSRSLITSGGLGTMGFGLPAAIGAKIAAPDRTVCLFAGDGGFQMT
                     IQELGTIMENNIGVKMIVLDNKFLGMVRQWQELFYEERYSFTKMKNPDFVAIGKAYNI
                     DGKLVNEREELDEAIADMLKDDRPYLLVVNVETKGMVYPMIPAGTSVTNIILE"
     gene            complement(10300..12129)
                     /gene="ilvD"
                     /locus_tag="PLD44_01090"
     CDS             complement(10300..12129)
                     /gene="ilvD"
                     /locus_tag="PLD44_01090"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOF97966.1"
                     /translation="MISRLRSTTSTSGRRMAGARALWRAAGMKNEQMGKPVIAIVNSF
                     TQFVPGHVHLHEIGQKIKVEIEKLGCFAAEFDTIAIDDGIAMGHDGMLYSLPSRDLIA
                     DSVEYMMNAHKADAMICISNCDKITPGMLMAAMRLNIPTVFVSGGPMEAGELDGVQLD
                     LIDAMVQSADDSISDEQIQRIENAACPTCGSCSGMFTANSMNCLTEAIGLALPGNGTI
                     VATHVNREKLFVQAAKLIVENAYKYYRDGDASVLPRSIATREAFLNAMKLDIAMGGST
                     NTVLHLLAVAHEGEVNFTMDDIDELSRHTPCLCKVAPNTQKYHIQDVNRAGGILGILA
                     ELSRGGLIDTNVRRVDGMTLKEAIHKYDITSPHVSAEIIQLYKSAPANRFNLVMGSQN
                     SYYKELDTDRTNGCIRDLDHAYTKDGGLAILKGNLAQDGCVIKTAGVDESIWKFSGPA
                     KVFSSQDAACEGILSGKVVAGDVVVINFEGPKGGPGMQEMLYPTSYIKSRHLGKECAL
                     ITDGRFSGGTSGLSIGHISPEAAAGGNIALLRDGDMIEIDIPARSIQVKLSEEELVKR
                     RQEELSRGKEAFTPKDRQRSVSKALKAYASMVSSADKGAVRLI"
     gene            12661..14553
                     /gene="dxs"
                     /locus_tag="PLD44_01095"
                     /pseudo
     CDS             12661..14553
                     /gene="dxs"
                     /locus_tag="PLD44_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965312.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
     gene            14676..16013
                     /gene="trkA"
                     /locus_tag="PLD44_01100"
     CDS             14676..16013
                     /gene="trkA"
                     /locus_tag="PLD44_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOF97967.1"
                     /translation="MKIIIVGAGEVGTHLAKLLARESMEITLSDENPDRLMDLESNYD
                     LLTSVGSSTSINHLREIGVENANLFIAVTPYESVNMTACMLATNLGAEKTLARIDNYE
                     YLFPENKDFFKKLGVDYLIYPEVLAAKEISESLKTSWLRQNMSFCNDALILLGIKVRS
                     NSEIINKKFSTGYFDHDKYRVVAIKRGMQTIIPKGSDEILDNDIVYFITTPQYLNFVR
                     EQAGKEDFPIKNVMFLGGSRIAQKTLKRLPDHIHTKVLEINNERSMELAEKFPDTLII
                     NCDGRNIDVLREEGIQEMDAFVAATGNSEMNLLACMVAKRFGVKKTIAEVENIDYIPL
                     AESMDIGTVINKKMIAASYIYQITLNASVLNVRNLTSADAEVVEFIAKAGSKITRSKI
                     KNLKLPENVNIGGIVRNGKGMIVSGETSIEPEDHVIVFCISSAIRKLEPFFNT"
     gene            16036..17505
                     /locus_tag="PLD44_01105"
     CDS             16036..17505
                     /locus_tag="PLD44_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017140927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOF97968.1"
                     /translation="MNREINFKLVIRMLGSLLLSEGTALLLVFFISLIYGENDGVYFI
                     FTSLLCYVVGFAGFFFGKNAPMSVGKREGSLVVTSAWIIFSLFGSLPFWLSKSIPLFT
                     DAFFETISGFTTTGASILNNIEELSHSMLFWRSLTHWMGGLGIIVISLALLPMFGTSG
                     AQLFAAESSGPTKDKIHPKISETAKRLFVIYFLLTFSETILLRLGGMTWFDAVCHSFA
                     TIATGGFSTKQNSIQYWDSPYIQYTIIIFMILSGVNFSLYYFGFKNKLQKIRENEELR
                     YYLIVLFGFAIIVALSMIDFSRPISFHKVEESWRNALFTVGSLMSTTGFTTVDYMQWK
                     PFVWVILLIVMLTGASAGSTSGAIKMVRIVIVFKFCYYEFKRLIHPKAVVPVRYSGKI
                     VFQDVVTRVLVFVLLYLIAAILGILILCMSGVNFIESVGGLVACLGGVGPGLGMVGPV
                     GNYSYFPVFAKWFLSFMMLLGRLELFTVLLLFTPAFWKK"
     gene            17597..20491
                     /locus_tag="PLD44_01110"
     CDS             17597..20491
                     /locus_tag="PLD44_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOF97969.1"
                     /translation="MESFLYRIAQTYYNHYEDKISDFTFVFPNRRAGMFFQRYISQIA
                     KKPLFSPEILTINDCFTSVAQFHLADRLSLLFRLYRIYKEKSGTNENFDSFAYWGEML
                     LNDFDDIDKYRIDARQLFTNVTELKQIDRMFNYLSENQLDAIRQFWKNFIPIPKEKTQ
                     EDFIQIWKILYPVYQDFTEELKRENMATEGMIYRNLAERLESEEKIQEWEGKQFVFIG
                     FNALNRCEKTLFEVLQKKEKADFYWDYEAPALRDADNPASTFFMENTKTFPSKFSIET
                     PVEERKKQYFELIAVPSEIGQIKQIYEILNRIYPQDASVQQESWIKTAVVLPNENLLL
                     PALYSLPEHIEKVNVTMGFPLKSTPVSGLIEHVFDLQKRIRNSDEEVLFYHQPVTEIL
                     QHSYITLLCGDDSKNILQQMVENNSIYIRPEQLQINELFTAIFRPQNDAREMLTYLLN
                     ILRILRKNFQQIGFGNHQYRMETDFVIQYYRFLNRMSDILQYQSEGIEMKMDTLMRLI
                     FQYSAFITLPFVGEPLEGLQIMGVLETRGLDFDNILLTSFNEGVFPKNAPTHSFIPYN
                     LRRGFGLPAKEHQDAISAYNFYRLIHHAQKVYFLYNSSTEGFQTGEVSRFLYQLEYLY
                     DTKVERKNITYDIFLKDAVSASVEKDSLIREKLQRFVSSGDDKLYLSASSINAYIDCP
                     LKFYYAQIARIKETEEITETVENDLFGTLFHSSAELLYKPFKDVQLGKEDFEKILRQP
                     SLVDEAISLAFSKHFFKNEQNGKIPPLEGNNLLISKILKKFLMRLLYVDQQLTPFRYL
                     GAEEFCSIRFPLETLNLRVNIKGYIDRIDETENFLRIIDYKTGTGNTTFKLLDDLFDG
                     SLPMKNRPKHIFQVFMYCLLYRSKAEKKIISPAIYYLKEIFSSGFSSSIQFKPEKHTI
                     QVEDFGIFEHEFKEKLTLLLNEIFNPEIPFKQTQIKENCDNCSFMEICNR"
     gene            20669..21142
                     /locus_tag="PLD44_01115"
     CDS             20669..21142
                     /locus_tag="PLD44_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOF97970.1"
                     /translation="MKIETERKFLVTGAFKSQASKSYRIIQGYLCSDPERTVRVRIMD
                     ISAFLTIKGKSNLSGLSRFEWEKEIPVDEAQELMKICEPGIIEKKRYEVVYRGQRFEV
                     DEFFGENEGLILAEIELENESQEVLKPDWLGEEVTGKPEYYNAMLKKNPYRSWKK"
     gene            21274..22041
                     /locus_tag="PLD44_01120"
     CDS             21274..22041
                     /locus_tag="PLD44_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOF97971.1"
                     /translation="MKKFFSLLSLFLFVSILSQAQNELFLKKLFIYKGDTLPYRILYP
                     ENYNKSASYPLVLFLHGAGERGNDNEKQLVHGASLFTDSQNRQKYPAIVVFPQCPENG
                     FWAPIKERDNGFSFINSKEPTPSMQMVIRLLKQIRKEEAVDSRRIYVMGLSMGGMGTF
                     DLICRQPKTFAAAIPICGGVSLERLKKVKHMPIRIYHGGSDDVVSPEHSRNAYIELKA
                     LGSTKVEYIEFPGVKHNSWDFAFAQPDFLSWLFSQKK"
     gene            22175..22402
                     /locus_tag="PLD44_01125"
     CDS             22175..22402
                     /locus_tag="PLD44_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOF97972.1"
                     /translation="MAKIKGTVVVDTERCKGCNLCVVACPPKALSLSKEANSRGYNYS
                     FLSNPDACVGCVACAYVCPDACITVYKMRVQ"
     gene            22450..23556
                     /locus_tag="PLD44_01130"
     CDS             22450..23556
                     /locus_tag="PLD44_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOF97973.1"
                     /translation="MNKNSKTDNKEEVLLMKGNEAIAHAAIRCGCDGYFGYPITPQSE
                     VMETLMELQPWTTTGMVVLQAESEVASINMVYGAAGCGKRAMTSSSSPGVSLMQEGIS
                     YMVGAELPCVIVNVMRGGPGLGTIQPSQGDYFQAVKGGGHGDYKLLTLAPASVQEMAD
                     FMPLSFDLAFKYRHPVMILADGIIGQMMEKVVLPPFQPRRTDDEIREQCPWATLGKPS
                     NRPQNVISSLALEAEAMEAINLRIQKRYREMEKNEVRYETYMADDADYLIVAFGTCAR
                     VSQKAVEMARENGIKAGLLRPITLFPFPKDMINQLASSVKGMISIEMNAGQMVEDVRL
                     AVNGKVPVEFFGRLGGIVPSPDEVYDALVSKLIK"
     gene            23631..24392
                     /locus_tag="PLD44_01135"
     CDS             23631..24392
                     /locus_tag="PLD44_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOF97974.1"
                     /translation="MSNNEIISPENLVYAKPKSLKETYMHYCPGCSHGVVHRIIAEII
                     DEMDIQDKTIGIAPVGCAVFAYNYIDIDWQEAAHGRAPALATAIKRLLPDRYVFTYQG
                     DGDLAAIGTAETIHACNRGENIVIIFINNGIYGMTGGQMAPTTLPDMVTSTTPYGRKT
                     ELNGYPLNITNLLAQLPGTCYVTRQSVQSVAGVRKAKKAIQKAFENSMLNKGTSIVEV
                     VSTCNAGWKMTPENANKWMEENMFPAYPLGDLKDI"
     gene            24529..25074
                     /locus_tag="PLD44_01140"
     CDS             24529..25074
                     /locus_tag="PLD44_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOF97975.1"
                     /translation="MKEEIIISGFGGQGVLSMGKILAYSGLMQGQEVSWMPSYGPEQR
                     GGTANVTVILSDEKISSPVLSSYDTAIILNQPSLERFESKVKPGGTLIFDGNGFHKFP
                     TRTDINVYRIDATEYATAHNAAKTLNMIILGGFLKVRPIVKIENVLEGLKKSLPERHH
                     HLIPQNEKALQIGMELIKKVN"
     gene            25338..26591
                     /locus_tag="PLD44_01145"
     CDS             25338..26591
                     /locus_tag="PLD44_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOF97976.1"
                     /translation="MNNFTAVLITLFFSAFFSGMEIAFVSSNKLRFELDKKQRSITSS
                     ILTIFYKHPQQYISTMLVGNNISLVIYGLLMAKILSPLLQILGSQFLITFTQTIVATA
                     LVLVTGEFLPKSIFRVNPNFWLSIFSPLLLFFYVILYPITLLATSLSFGIMRLMGIKI
                     NPLANENAFSRVDLNYLVHESFENTEHKQDVDNEVKIFQNALDFSKVKLRDCLVPRTE
                     IVALDYNVDLETLKQTFIETGFSKIPIYKENIDNIIGYIHSSEMFQHQNDWREHINQI
                     PIVPENMAAQKLMKIFLKEKKSIAVVVDEFGGTAGIVTLEDIMEEIFGEIEDEHDTHD
                     FVAEKVGENEYVFSGRLEVKEINEKFHLNLRESDAYDTIAGFILYHHQHFPKLNESIQ
                     IDNYTFKCIKITNNRIELVKMKLQK"
     gene            26715..28829
                     /locus_tag="PLD44_01150"
     CDS             26715..28829
                     /locus_tag="PLD44_01150"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOF97977.1"
                     /translation="MATLEKIRSKGVLLIVVIGLALLAFIVGDFLNSSSTFFNQSKEK
                     IAVIGDENISIHEFSAAIDQMTEVYKIETGQTNLNEEVVSQLRASVWENMLMEKILTH
                     EAKKIGLSVSPEELSERLIGKNIHPIILQRGAFMDETGQFSRSLLVQFLNSLDVTPTN
                     NEMKQQIDQAKNYWLFWEKNVKNAILQEKYNKLITSAISANSIDAKMNFEARKITVDA
                     AYVMQPYYSVPDSIFKVSSKELRDLYNKRKELFKQEKSASINYVALEIKPSQEDFQEA
                     EEWINKLEDEFRTTDDVVGLVNTNSDIMYDGRYYSEATVPAMLKDFAFRGKKNDVFGP
                     VFENDTYTMARIMETDILRPDSVKLRHIFLTQENEGKADSIVKALRAGADFATLARKY
                     SAVPQTAANGGEIGWLPDGAQGVDKQILENAFKKPLNEVFVIKDIQGTQIMQVTDRTP
                     LRRKVKLAILQRKVTPSSKTYSQIYNQAKQFAAELDAEKFVSRAQEKGYIVQKAENLN
                     ENTEQINNLPQSRQIVRWAFKGGKGDVSDVFDCGNYYVVAMITEKNEKGYMPIEKVSA
                     QLKSEIIRDKKAAQMINNISEQLKKTPDLEALAASLNAEIKMAENVNFTSYQFGLAGF
                     EPYIIGKATIVPLEKVSGPFQGNAGVYVMKTSNKKENTETFNAQQEIIQLNARMLYSL
                     PYLILENLRERTKIEDYRSNFY"
     gene            29049..30074
                     /gene="rlmN"
                     /locus_tag="PLD44_01155"
     CDS             29049..30074
                     /gene="rlmN"
                     /locus_tag="PLD44_01155"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOF97978.1"
                     /translation="MPIPLLGASLEELTDIVSQLEMPTYTAKQISDWLYKKRVTDINE
                     MSNISVKNRDLLKEKYKVGRLAPVKVTVSKDETKKYLFRTEKDHFIETVFIPEEHRNT
                     LCVSSQIGCKMNCVFCMTGKQGFMGQLSASEILNQILSVPEAEKITNLVFMGMGEPFD
                     NTVELLRTLNILTANYGYAWSPYRITVSTIGLIPGMQTFLEKSNCNLAISLHSPFHSE
                     RLELMPIEKAYPISQVVEVLKNYNFGRQRKLSFEYIMFDKFNDTMRHASELVRLLKGM
                     NARVNLIRFHAIPNVSLRSSSDERMIWFCDYLNNNGILATIRKSRGEDISAACGLLST
                     LENKKMS"
     gene            30255..33176
                     /locus_tag="PLD44_01160"
     CDS             30255..33176
                     /locus_tag="PLD44_01160"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOF97979.1"
                     /translation="MEKISVDNIYINNREKKIIIKANEKFGYMPFREENVKHIYRVLK
                     QIFSPSYKNYKIVCMVDGQTIEELIPNFYRTKRKRDETRIFDPKKSKYSLVKNLSKPY
                     NVTNGLERKNIALWASHGLYYEQSISRWEWQRARVMQTVEDLFTLSIVYPYLIPMLEN
                     AGATVLTPRERDMQSNEVIVDNNNKDSLSYREFNLQEVWTESQDFGFANFKSFYLQGE
                     NPFNMGSYRQIHSVEKENKASFVEYIPDFPKAGKYGIYVSYKTLPQSTENAHYTVYHK
                     GGKTKFSVNQTMGGGTWIYLGQFNFDKGRNENGKVVLSNFTGEKEEIVTADAVKFGGG
                     MGNIARSPDQNGILENHKSSEIKNPALKKEMVLSWETEAEISGYPRFAEGARYWLQWA
                     GFPDSIYSPNRGANDYIDDFQSRAKWVNFLTAGSSLHPKGKGLKIPLDLSLALHTDAG
                     TTKNDSVIGTLGIFTVGKGNGKSYNNKVSRWASRDLTDIIQTQIVEDIRMSFNDSWIR
                     RGLWNKSYYEARVPEIPTMLLELLSHQNYADMKLGLDPRFQFLVSRSIYKGILKYLSA
                     LNDEEDYMVQPLPIRQFSCIFVDKNKVKLRWKTRMDSLEPSAVAEHFVVYTRIDDGDF
                     DNGVLVSDTEYLKVLEQGKIYSFKVTSVNRGGESFPSEILSVCSHPHEKGSVLIINGF
                     ERVAPPAGFVIDTTYAGFLNDMDAGVPYLYGYNFTGKQYEFRRDRKWVDDDAPGFGAS
                     HANYETQILAGNSFDYPFLHGKAICAAGYSFVSCSVEAVMNDDINLNDFQTVDLILGK
                     QKQTDVGTRENISLFKTFPLALQQKINLFCHQQGGNLLVTGASIASDMFDRNDALPDD
                     IRFLENTLKIKYRVHQAAITGNVKFVSSPFKQFVRHQFSYYSEPNQYSYFVESPDAIE
                     PAARDAYTFCRYAENNLSAGVIYSGKYKICALGFPFETIVSDAEREAFMKSVLDFFAK
                     P"
     gene            33263..34381
                     /locus_tag="PLD44_01165"
     CDS             33263..34381
                     /locus_tag="PLD44_01165"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOF97980.1"
                     /translation="MKRAFLRFHARIVERIRVSNISTLKFNFILTKTKTTFVLTLLLI
                     MSGCNSNKSSLPGITGSMYEVLVVMDHQQWISAPGRTLVDLLDQDMEALPQPEPVMDI
                     IPCRPEGFSNMFKSSRNIIISDISEKYSQPKVVYARDKWAHPQAIVRITAPNDSSFVE
                     AVQKFGKNILDFFITQERQRQMEYNKSFVNLEARAEIEKIFGIQIDIPSEFKKAVKKK
                     DFYWITNDNPKIRKDIVIYSYPYTDKNTFTKDYLLAKRDSVMKYNVQGEIEGSYMGTE
                     YKYYPPIFREIWVNGGYCAEIRGLWKMFHGAAMGGPFYSHTRLDEINQRVITIEGFVF
                     APGEKKRNAIRQLESVVYSAKLPQEINALKEISVTPKK"
     gene            34405..35463
                     /gene="pdxA"
                     /locus_tag="PLD44_01170"
     CDS             34405..35463
                     /gene="pdxA"
                     /locus_tag="PLD44_01170"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOF97981.1"
                     /translation="MEQKIILGITHGDFNGIGYEVILKALSDPVILDFFTPIIYGSSK
                     AAGYYRKMLDIQNLNLNIIGSVKELNHKRVNIINCVDENIKVEVGQSTPEAGEAAYIA
                     LERAVEDLKNGEIHALVTAPINKKNIQSEKFHFPGHTEYLAQHFGNTENALMLMVNDV
                     MRIAVVTGHIPLREVPSALTSELVLKKLQILNHSLKEDFMVVRPRIAILGLNPHAGDD
                     GLIGDEEKTILLPAMKEAEKAGIICVGPYPTDGFFGSGSFTKFDGILAMYHDQGLTPF
                     KTISMDNGVNFTAGLPIIRTSPAHGTAYDIAGQNVASENSFRQALYLACDVYKNRELN
                     HQLKSNQLKFESKEKENG"
     assembly_gap    35610..35709
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            35871..36983
                     /gene="ald"
                     /locus_tag="PLD44_01175"
     CDS             35871..36983
                     /gene="ald"
                     /locus_tag="PLD44_01175"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOF97982.1"
                     /translation="MKIGVPKEIKPQEGRVAMVPAGVMDLVCRYGHEVFVQKGAGEES
                     GFSNEAYLSAGAVMLDTIEEVYNMADIIVKVKEPIEREYSLIKRNQILFTYFHFASSK
                     KLTEAMMQSGAVCIAYETVMEKDGSLPLLMPMSEVAGRMSVQQGAKYLEKPQNGKGIL
                     LGGVPGVRPANVLIIGGGVVGTQAAYMAAGLGANVTMLDVNLYRLRYLDDVMPANVIT
                     QYSSKYAISELLPTADLVIGAALTVGAKAPHLITREMLKLMQKGTVMVDVSVDQGGCF
                     ETTHPTTHTDPVYEVDGILHYCVANIPGAVPITSTRALTNATLPYVIKLAELGWEKAC
                     KNHEDIKHGLNIVDGKIVFKNVAESLELPYTEYQIS"
     gene            36985..37962
                     /locus_tag="PLD44_01180"
     CDS             36985..37962
                     /locus_tag="PLD44_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOF97983.1"
                     /translation="MVHWGFIGCGDVTEKKSGPAFQKVEGSDVVAVMSRRLDKAKDYA
                     LRHGVKKWYDDADKLIHDPEVTAVYVATPPSSHAEYAIKAMKAGKPVYVEKPLASSWL
                     EGQKIVQVAEETHVPCFVAYYRRTLPYFLRVKKLIDEGTLGKISSVQIRFALPPSQND
                     FRPDTLPWRVKKEIAGGGYFYDLAPHQFDLLDFILGPISEAQGFTTNIAGLYEVEDAV
                     SGIFRFSNGILGSGMWTFSAPENFRCDEILFVGTKATLTCSTFDFSPIVLENGEKVEQ
                     FSEPKPENIQFYLIESIINFLNGKGTEPVSNCYSAIRTNWVMDTILKKI"
     gene            38162..38653
                     /locus_tag="PLD44_01185"
     CDS             38162..38653
                     /locus_tag="PLD44_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOF97984.1"
                     /translation="MHKILLLMSVIMTLHMSVFSQKSEPSTIVQSDGLFFTDLSQTKL
                     YTGDYREFYDNGSLRLEMYIKDGKPEGDYIIYFPNGKPNEIRSYRNGKFHGTWRTYNE
                     SGVLISEAFYRNDKKHGTWHIWDDNGIMRYEMHYHEGEKIGTWFMWDEKGKLISEKNY
                     STP"
     gene            38762..39109
                     /locus_tag="PLD44_01190"
     CDS             38762..39109
                     /locus_tag="PLD44_01190"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOF97985.1"
                     /translation="MKKNFFAVVFFALSVFLGGNVMAGENNSSEAKNVSNTKTVIEGK
                     VVDKNTLETLAGVKIMLNGQKVYTDLDGRFTISTFPTGKARLKASLISYEEQDVEIDL
                     STSNSLQIKLAQH"
     gene            39428..40117
                     /locus_tag="PLD44_01195"
     CDS             39428..40117
                     /locus_tag="PLD44_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOF97986.1"
                     /translation="MAPTRILVVDDEEDLCEILQFNLETEGFQVDTAYSAEEALKKDL
                     TVYNLILLDVMMGEISGFKMARMLHDNPKTASIPIIFLTAKDTENDKITGFNIGADDY
                     ISKPFSLREVIARVKAVLRRSESMQMDETTNKILTFEKLEMHLNNKKVSLEGNEIPFT
                     KKEFEILKLLLENKNRVFSREEILAKVWTDDVCVLDRTIDVNITRLRKKIGPYGKNIV
                     TRLGYGYCFEG"
     gene            40189..41916
                     /locus_tag="PLD44_01200"
     CDS             40189..41916
                     /locus_tag="PLD44_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF97987.1"
                     /translation="MKLNKKIFLYFFSIFFILFVIVTYFQYHREKEFSRSQLDGQLNS
                     YVHTIHNFIQSEQPTWEELSRFVRLFPDSTLRVTIVDSLGNVIFDSFLPKGTLFQNHL
                     DRPEIAKARKEKIGEAIRYSASTGKNYYYMAQRFPEYYIRCALPYNVNLIAALRANMF
                     FLYFMLFMFVITVFALFIISKNFSRSIDRLRIFTQKAEQDDILDTDIQFPNDELGEIS
                     NNIVQIYRRMLESKADADRQREKLFKHLQISREGLGIFSSDKKEILANTHFIQYTNIL
                     SDQEVKSSDQIFSISEFSEINAFIDDCFSNNETKRKRITIEKNGHSFYVQCIVFQDDT
                     FEISINDISAQEHENELKRHLTQNISHELKTPVSSIMGYMESILENPNISPQRERFFI
                     ERAYQQTQRLNALLQDISTLNKLDEAYKLFAKEPCSLSEVIDEVLNDVHVQIEQKKCR
                     IIKNYPSDMKINGNNSLLYSIFRNLIDNALTYGGDNITVEINCYREDNEFYYFSFSDN
                     GRGVAEEHLNRLFERFYRVDKGRSRKFGGTGLGLAIVKNAVLFHKGNISAKNQPGGGL
                     SFIFSLRKF"
     gene            42148..42372
                     /locus_tag="PLD44_01205"
     CDS             42148..42372
                     /locus_tag="PLD44_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HOF97988.1"
                     /translation="MQRVGFLKNIYSFYSEGFKNMTVGKTLWIIVLIKLFIMFGILKV
                     FFFPDYLNEHFRDDAGKANHVRMELINKSK"
     gene            42396..43955
                     /locus_tag="PLD44_01210"
     CDS             42396..43955
                     /locus_tag="PLD44_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOF97989.1"
                     /translation="MELLLDASLVNWSRAQFAMTAMYHWLFVPLTLGLGMIMSIMETL
                     YVKTGKEEWKKAAKFWMTIFGINFAIGVATGIILEFEFGTNWSNYSWFVGDIFGAPLA
                     IEGILAFFLEATFISIMFFGWKKVGKNFHLASTWLTTLGATISALWILVANAWMQYPT
                     GMTFNPDTVRNEMIDFWSILFSPVAINKFFHTVISGWVLGALFVVGISCWFLLNKREK
                     DFALRSIRVSALFGLISSLLLLYTGDGSAYNVAQRQPVKLAAMEGLYEGKNGAGLIAI
                     GILNPEKKSYNDTLAPYLFKVEIPKLLSFLGYRDANAFVPGLKDIIEGGYQTASGETL
                     SFEEKRERGKQAIQALADYQQASQKKDTVAAKMYKDRLMKNYAYFGYGYLDHPQQLIP
                     NLSLVFYPFHIMVILGIYFIVLFAGVLILSYRKKLEHLSWLQWICLLTIPLGYVAGEA
                     GWMVAEIGRQPWAIQDILPVQVAVSSISAESVRLTFFLFLILFTSLLVAEIGIMVKQI
                     KKGPEAEKIEE"
     gene            44003..45151
                     /gene="cydB"
                     /locus_tag="PLD44_01215"
     CDS             44003..45151
                     /gene="cydB"
                     /locus_tag="PLD44_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOF97990.1"
                     /translation="MITYSFLQHYWWFLIALLGGLLSFLLFVQGGQSMIFSLSKTEDE
                     KRLLVNVLGRKWEYTFTTLVTFGGAIFASFPLLYSTSFGGAYWVWMIILFCFTLQAVS
                     YEFQTKAGNLLGKNTYRFFLFLNGLLGTFLLGVVIATFFTGSDFVVDKANISNPLSPI
                     ISRWQHPWHGLEALLNLRNVCLGLAVFFLSRTLAALYFINRVDHEMLEKRSRKYLWYN
                     AIPFVVFFLAFVIWTFLSDGFAVHPVSGEVYLSPHQYFLNLVEMPAVMTTFLLGIVGV
                     LYGIIRTLFSENYRKGIWFAGAGTVLAVLGLLLLVGFNNTALYPSATHVQSSLTLSNA
                     SSSEFTLKVMSIVSLIIPFVVIYIFYAWRALEKKRTNVEEINEDPHAY"
     gene            complement(45399..46529)
                     /locus_tag="PLD44_01220"
     CDS             complement(45399..46529)
                     /locus_tag="PLD44_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF97991.1"
                     /translation="MKIIRRILQDKIIDKIAPGKVVLIFGARRVGKTILMHQILESYQ
                     GKTMLLNGEDYDTLTLLENRTIANYRHLFNGIDMVAIDEAQNIPQIGSILKLIVDEIP
                     GISVLASGSSSFDLLNKTGEPLVGRSSQFLLTPFSHREILQMETMLETRQNLEARLIY
                     GSYPEVVMMDNFDRKTDYLRDIVGAYLLKDILAVDGIKNSSKMRELLRLIAFQLGNEV
                     SYDELGKQLGMSKTTVEKYLDLLEKVFVIYRLGAYSRNLRKEVTKAGKWYFYDNGIRN
                     AIIGAFSPLAIRQDVGALWENYLISERRKANLNEELHKEFYFWRTYDKQEIDLIEESS
                     NDLTALEFKWRNKIPKIPKAFQETYPHAKFYVVNKDNYLEFI"
BASE COUNT        14905 a         8516 c         9093 g        14650 t
ORIGIN      
        1 ttattattat aactatcaac aaaaatgaat ataatttttc aaatcaatac attgtaaaag
       61 ccacaatatc aatgttaaaa aacaaacaaa aacttaggga ttagactttt tttgtatatt
      121 tgcatcttag aggcttgaaa aagtttcgtg ataaatcaat ttatagcaat gaaaaaatca
      181 caattcaata ttttacaaac tttcgcatta cttataattt caataattat gagttcgtgc
      241 gaggatttct tgtcgtctat acgcaatgag aatagccaaa gcatagaatc gttgttatac
      301 aatataaatg acattgatgt ttgtataaac ggtgcttatg gagctttttg ctcaaactct
      361 tattacaaca atgtagtttt aactcaaaca ctaggatcgg acgagattac gaacaccaaa
      421 aacccaagca tattacctga ttttctatct atctcagcgt acaattatca cttcagctac
      481 cagtgtaaca ctgataatta tcaggctggt tcagttttaa tgtggggatc atttgtcacc
      541 aacaacagta atgtagtgat caagtcaatt gaaaataaat taccaaattt acgtaatttt
      601 aatgacacgc tcaatgccga aagacttttg ggagaggcat atttattgcg tgcctgtgtt
      661 gagtattaca acaacttgta tgttgggcgt caataccacc caagcacact tcaacttcca
      721 gcaagcttat atagaaaaag agccatttta ggcttcgaag atatacccga acctcgaaaa
      781 acggttgaac aggtgcatca gtatattata gaagatctac aaaatgcacg caaactactg
      841 ccacaatatt acgaaaaaac aatacatccc gtagcatatc aatatcggtg taagcgagac
      901 gtagcgatag caatgttagc taaagcatat tttcaacaaa accaatttga ttcggctttg
      961 gtatatatca accaattgct tggaaatcaa acgggagctt cgaataaata cccgcttcaa
     1021 actacgaata actataatca actttttcga attgtagata aaacaaatta tcaagctaat
     1081 agtaaaagtg aaattattat gggttttcat gggaactctg cattccgtcc tacatctaca
     1141 caagagtgga gcatgtttca ttggagtgcg tttcaaaatc ttcaaaatag cgacttaaca
     1201 aatctccgtt tcagaattgt tatggacagt gcattaatta aaagtttttt gaaaggcgat
     1261 actttaaacg atgttagatt taaaaacatg atatatatta cgaaaaacat aggaaaagaa
     1321 tcgcctgcag ggcagtggac tagtctaaaa catgcatacc caacttcaaa tgtggtatgg
     1381 cttcgtgcag cagagtttca tcttatgcga gcagaaattt atctccacaa ggataatctt
     1441 agttcttcaa ttaacgaact taacatagtt agaaaacgtg caggacttga caattatact
     1501 ttcgcaggaa aaactcaatt atttcaagca atcatcgatg aaagattcag agaaatgcat
     1561 ttcgaaggta ttagaaggag tgataatatt cgcttagcat ctttgagcga tagtaatgcg
     1621 gctagttact tacccatgcc atacaaattg ggtaatatac ctttaggaaa tagagctttg
     1681 ttaataactg acagtatatt acattggaac gacgaaagac tgtactgctt aatacctgag
     1741 aatgaataca tttacaatcc tgcactaaaa agataaagaa taataaaatg tatataatcc
     1801 gaataagtat aatgcacggc aagctataat acggagccta ttatgtcaaa aatcgaattg
     1861 agaagaaaac aaaacatatt caatcgctta ttactcatcg taattatcat ttgtttaaat
     1921 cttgcgagcg ctaaagcgtg ggcacaaaaa catgatgaaa aaatatcgtt aaatttcgaa
     1981 aatgctgatt tcaagcaaat aatgcgcgaa attgagctta aatgggatgt agtatttgtt
     2041 tacaacacat cggacctgaa tgaatttggc attaaaagtt tacagacaaa taatcttgat
     2101 ttgtatgaga ctttaaatac attgtttgca ggaaccgaca ttacttatac gataaaagat
     2161 gtttatgtgt ttttgcgtgc caagaaagat actaacaatt tgactctaaa agagtctaaa
     2221 agtattatgg gcgtaattgt tgatgaatta caacaacaat tacctggcgt ttacatcacc
     2281 gacaaacaag gcaacattct aggtataagt aatgaagatg gcagttttaa tatttatagc
     2341 ataccaaaag attcaagctt atattttttt atgcatggtt tcaagcgaga aacactaaaa
     2401 cttttgaata acaataacat ttacaaagtg acgatgcgct ccaacaactt attgctcagc
     2461 gagttattag ttatagggta tggcagcatg caaaaacgag aaactacagg agctatacaa
     2521 tcattaccga aatttgcaat taaaaacaat gttgggggaa atattgggaa tagtttcgaa
     2581 ggtaatgttg caggactttg ggcatcgaat caaaattatc gcataagagg tgtttcgtca
     2641 ataaactcgt cagccgaccc acttgttgtt atagacggga ttcctcaaac cattggctta
     2701 aatgacatta accctaacga tattgcttca gtagagtttc taaaagatgc agcctcagcg
     2761 gctatttttg gctcacgtgc ttccaatgga gttattgtta taagcacaaa atcaggtgat
     2821 ttgaattcga aacaaaaaac aattgccgaa tatagaacat caatcaatta cgtaacgaac
     2881 aaaccaaaat tcattaaggg caaagaacta atttcattac ttgatgcagc ttactataac
     2941 aggtatcctg aaaataaaac attagaagat aacaatccta tcaaatattt ccctttttca
     3001 ccagattatg ctaatttttt aggttttaac cgcgattggt tgaatactta tttgcttaac
     3061 aaccctcttg gaaccgactg ggaatcggca ttaacacagg ctgcaatttc tcaagatgtc
     3121 cgcttatcgt ttcaaggtgg acaaaagtca aatagttact acattagttt atcgcataaa
     3181 tcggacaatg aactagttga taataaaaac aagaaaagaa gcactttagt attaaaaaat
     3241 gattttaaga tatcggagag aattaacact ggatttaaca taaatttcac tctaaatact
     3301 tcgaaaaatt caacttttga caagttggag tctttatttt cacgttcttc attgctccca
     3361 atatatgcgc cagatggttc gggtaaatat ttcgacgcac gcaatataaa tgacaaaaaa
     3421 ggcagtaatc cactttatag aatgagtgaa acttgggatg acaatattaa ttttaatggt
     3481 ttattacttg gttatttcga tattaaattg atgcaaaaat ttgcatttag atctgaactt
     3541 agtatgacac ccggtactag caggtatcga tattttcagt caaaagactt ttatcgcgaa
     3601 gatgaagcta tcgacccatc aaaaagcggt ataatactat attcaagaac cttaagctat
     3661 ggatttaatg taaacaattt actgaattac aatacaaaaa taaacaaccg gcatgtcata
     3721 aaagttatgc tgggtaataa cattcaatcc tttaacagcg attttaatgt tgcacgcttt
     3781 gagggttttc caaccaatta ctttcagcta actaatgcaa acaccgaaaa agtactaaca
     3841 cgacaatcgg caggaatgga tggctatcga tttgtatcct tttttaacag aactcaatat
     3901 gcattagacg agaagtattt ttttgaatta aacgcacgag ccgacggcac atctcgattc
     3961 attaatgata atagatgggg gtttttcccg gggcttggaa tgagttggat gttaagtgaa
     4021 gaaaactttt tttcacagat aaaatcaatt gattatttaa aatttagaac aagttatggt
     4081 tttgtaggaa atgcagaggt tggtaatttt cctgccgaat cgcgagtact caattgggct
     4141 gaatacgcag gtagccctgg ctttgtgttg gacagaatag caaacttgga tgtgcgttgg
     4201 gaaaaacaac tacaattgaa tgtaggattc aatatttcag ctttaaaaaa caaaatatca
     4261 gcaagcctag attggttttt aaaacgtaac tctgatttac ttttgaatta caatattgga
     4321 aaattccaag gatactttaa cacagaagtt ccaataaatt cgggtaattt gaataacaat
     4381 ggtttcgatt tagatttaaa aactaccaat tatgatggtt tattcaaatg gaaaaccgaa
     4441 tttaacattt caatgtttaa tacaaagatt tcaaaacttt cgactcagca aaactatatt
     4501 gaaaatggaa taaatagagc ttatgtgggt tacccactag gtttatacta cctaccatta
     4561 tgggcaggtg tcaaccccga aactggacat gaaatgatat atgaagttat aggacccgaa
     4621 acaaataaaa ctaaaacagg taaattattg gatgccgaaa tgcttacatc tgttgagtat
     4681 cgcaaccagt ctgtattgat aacagacaag acaccctatc ccaaattata cggaggagtg
     4741 agtaatttct ttgcatacaa aaaattcgaa ctcaacatac acttttcatt tcaactcgga
     4801 aattggatat atcacaacgg tatgcgacaa aattcatacg taaacaccta tgacataaac
     4861 aataagatgg tttcgctaca aaattattgg acgcctgaaa ataaaaactc aaacacacca
     4921 cttctttata actctcaaat ggcgaaccgt aatagcacac gttatttgat gaatgcatcg
     4981 tatttgcgat tgcgcaattt gagtatgaac tatactttcg actcaaaaaa caccaaaaaa
     5041 ttcggacttg gaaaaataat agctcaaatt aattgtcaaa acttaataac attatcaaaa
     5101 ttttcgaatg gttcacctga gttgtcatta caaaatgcag gcgctgaagc aaacatatcg
     5161 ctaggaaata ttgggcttaa ctatggagtt gctacattaa gtgctggaat taaacttgaa
     5221 ttctaatact tttcctctca tttcatctca tctcataatc ccaaattgtc cattagagct
     5281 atcgagtaga aaaatcgaat taaaaattcg atgatttttt caaagttttc gtaaaaatta
     5341 ttaacggtca tttaaaaaac agaccatcgc ttaaatcgta atttctacaa aggaattaca
     5401 tgcgataaaa tgtaatgctt gcagcagttg catttaactt taagaggatg atgaataaat
     5461 agaaatcatc tttttggctc gaattaaaaa aaaattgttg acttgataaa cgctatgttt
     5521 taccaagtca ataataactt aataaaaaat ggacttttca aggctcgact gattattttt
     5581 catgtttccg cctttcaaaa aagaaagacg caggctttag ctttgtcttt tttacagaat
     5641 taaatatcac tgataatctg attatcaaaa caatacgatt atcttattca aaatattaac
     5701 ctaagattta tagaggaaag atatgataat atgtaactta caaccttttt aacaaccaat
     5761 ttgacttact agtcaaaatt aggacatttt tcttaatcaa tttattcttc cttcggcgaa
     5821 aaataagatt ttaatttttg ctccaacttt ttaatttaca cagttggtga aatactgcca
     5881 tgattgtcat ataaacaaca tgaaaataat ataataaagc taagagggct ttttataaaa
     5941 aacctgtcgt ttgaattaat ttcaaaacag acaggttttt agtgaaattc ttaatcaaaa
     6001 aaatttaatt gttttcgggg cgaagttttc taacaacagc gcctgttctc cacatttcgc
     6061 tttcgcgcaa agccttaagt tcctcttcaa gccgttccct gtaatcgggg cgggaattgg
     6121 aatcaatgga acgttgtgct tcacgacctg aagccacttc gttataaagt ttttcgaata
     6181 ccggtttggt ggcatcgtgg aatgggccca tccagtccaa tgcaccgcgc tgagctgtag
     6241 tggaacagtt ggcatacatc caatccatac cgttttcggc aaaaagtggc atcagcgatt
     6301 gcgtcagttc ttccacagtt tcattgaaag cttccgaagg agaatgtccg tgtgctctga
     6361 gcacttcata ttgagccgac aaaagtccct gaatagctcc catcaaagtt ccgcgttcac
     6421 cggtaagatc ggaataaact tctctttcaa aagtagtttc aaataaatag cctgaaccta
     6481 cacctattcc gagtgcaatc actctgtcaa aggctttccc tgtagcatcc tgaaaaatgg
     6541 cgtaggaaga attcaatcca cgtccttcga gaaacaatcg acgtaaagaa gttcccgaac
     6601 ctttgggagc aaccataatt acgtcaatat cgggaggagg aataatgccc gtacgatcct
     6661 taaaggtgat gccaaatcca tgagaaaaat acaaagcctt gccgggtgtc agatacggtt
     6721 ttatacgtgg ccaaacttct atttgggctg catccgaaag caaatactga atgatggttc
     6781 ctctttctgc agcttcttca atatcgaaaa gtgtttcgcc gggaacccag ccatcggcaa
     6841 cagctttttc ccaagtttta ccgcccttgc gttgtccaac aatgacatta aaaccattgt
     6901 cccgcaaatt gagcgattga cccggacctt gaacaccgta accaattacg gcaattactt
     6961 catccttcag tacttcacga gctttgctca aaggaaattc ttcgcgggtt attacatttt
     7021 cgtcaacacc gccaaaatta attactgcca tttttttgta gtttttaaaa attatttgtt
     7081 attaagttgt ttgttatcaa ttgtttgcta aaaattttat ttgttacttt tccgaaaagc
     7141 ttattcttgc tttgcaagtc gtttttttat cactgtctat ttcgaaataa aaatcatttt
     7201 tcttcttttc ttctcgaaaa attttaataa tttcgttttc atgtaattcg gaaatatagt
     7261 gaatctcaaa gcgttcaatt tgcttttccc gatagtccga caaattgaag caattgcata
     7321 gccattgtac gtaacggata ctgtttacat gtccgttaat atcgatatcg ccatatttca
     7381 cggttatttt gtctaccagc tcacctttca cgggaggaag tttctccaat ttaccggccg
     7441 agatttcttt tcctgtggca aattcttcaa aaccttccag agaaagcaaa tcttttggtt
     7501 tgcgttgggt caaatccatc ataatccatt ctgaagtggc tgaggcaata atttctccct
     7561 gtgcattttc aatacaaaaa ttgcgtatgg tttttatcgg ctgaattttt tcaatccatg
     7621 tcgatatttt tatttcgtca tattgttcag ggtaataatt tatttccaat gcaattcgta
     7681 gcaccaccca cgaagcattg aattcattca attgccgcat cccaaaacca tttccatcgg
     7741 catgtctgcc tgctgcttcc attaaaagct ctattagcga ggctaaagtg agctttccct
     7801 gaaaatcgac attggacgga gcaattttaa aaatatatga aaactctttt tccactcgta
     7861 tttttaagtt ggtattgcaa caatttgtcg ttgcaattat tcttgtgttt cggttgtggc
     7921 attgactttt tgttcaagtt tactcatttt ttcgagcata tcgctcagcc gttccacttt
     7981 cgacttggtg atggcaattc gtcccgaacg aataaattgc aaaacgccaa tagtcgagct
     8041 caattcttca aaaagcttct gtgtttcctg ataatgtccg gttttctcaa aaaccacaca
     8101 gttttcggtg agttccaaca catgaatttg gtaatctcga atcatttttt cgatagaccc
     8161 caattcgagc aatttgtccg ttgccacttt gtacaatgca atttcctgat gtacaagatc
     8221 ttcatcggta ttgtagtaag ccttcagaat atcgacgcgt ttgtcgattt gctgcactac
     8281 tttttcgata acatcctgtg tggtaatgac ggtaatcgta aatttatgaa tacctgcaat
     8341 ggccgatgga gaaaccgaca aagtctcaat attcagacct ctacgcgtga agatgacggt
     8401 aatttggttc agtacgccaa ccgtattttc cgaaaaaatg ataacggtat ataatgtctt
     8461 ttccataatg caaagtttta aaaaatgctg ataaatctct gaagttatat ttttgaaatt
     8521 tcagtaataa atctactcca gaataatgtt ggttacactt gttccggcag gtatcatcgg
     8581 gtataccatt ccttttgttt ctacatttac aaccaacaga taaggtctgt catccttcag
     8641 catatcggca atggcctcat cgagttcttc ccgttcgtta accaattttc catcaatatt
     8701 ataagccttt ccaatagcta caaaatcggg atttttcatt ttggtgaaag agtagcgttc
     8761 ttcataaaat aattcctgcc actgacgcac catgcccaaa aacttattgt cgagcacaat
     8821 catttttacg ccgatattgt tttccataat ggttccaagt tcctgaatag tcatttggaa
     8881 accgccatcg ccggcaaaca gacaaaccgt tctgtccgga gcagcaattt ttgcgccaat
     8941 agctgccgga agtccaaaac ccattgtgcc cagtccacct gaggttatca aacttctgga
     9001 ttgtgaaaat ttaaaatagc gagccgatgc catttgattt tgccccacat cagtaacaca
     9061 aatagcattg tgctgagtgg cttcggatac tttgtggata acttctccca ttgtaatttt
     9121 tccgcttttg gggaatacct cgcgttcaat tactttttcg tattccgttt tttcaaaaat
     9181 atcaaaggaa tcaatccatt cggtatgttt ggcaggttta acaagttttg ttaaagccgg
     9241 tagcacgtct tttaccgagc ccaaaactgg cacttccact ttcagatttt tattcagttc
     9301 agctgcatca atatcgatat gaattatttt ggcttgactg gcatacgttt tcagatcgcc
     9361 tgtaacacgg tcgtcgaagc gcataccgat ggcaattaat acatcacatt cattggtttt
     9421 catattggga gctacatttc cgtgcatgcc tacaaatcct ttgttcagcc gaaagtccga
     9481 tggcatggcc gaagccccca gtaaagtcca tgcagccgga atatcggctt tttccaaaaa
     9541 ttccatcaag tctttttctg cctgaccgag aatcacgcca tgaccaacca cagcaaaagg
     9601 tttttcagcg gcattaatca gttcggctgc tttttcgatt tgggaaaaat taacttctgg
     9661 aaccggaaca tagctgcgga taaatttaca aggtttgtat tgaaaatcta cttcttccga
     9721 ctgtgcattt ttggtaaaat cgagtactac aggtccggga cgtccggtgg atgcaatgta
     9781 aaaggcgcga gcaactgccc atgccacatc ttcggcccgc cgaatctgat atgaccattt
     9841 tgttataggt tgtgtaatac caatcacatc aatttcctga aatgcatccg taccaagcaa
     9901 acttgctcca acttgtcctg taatgactaa caatgggaca ctgtcgagca tggcgtcgct
     9961 gattccggta atagcattag ttgctgccgg tcccgaagtt atcaggcaaa ctcccacttt
    10021 ccccgaaacg cgggcatatc cttcagctgc atgagcggca ccctgttcgt gacgcgtaag
    10081 aatatgattt actttccgat catagtcgta taaagcatca aaaacgggca aaatggcacc
    10141 gcccgggtat ccgaatatgg tttctactcc ttcttgcagc aatgattcta tcaatgcttt
    10201 tgctcctgtc attttcatat tattattcct ccttttttca tttgggaatc tttgaatcaa
    10261 ataataaact ttttgtactg taaaaaatca tttttcattt taaatcaacc gcactgcgcc
    10321 tttatcggcc gaacttacca ttgaagcata ggcttttaga gcttttgaaa ccgaacgttg
    10381 acggtctttc ggcgtgaaag cttcttttcc tctcgacaac tcttcctggc ggcgttttac
    10441 caattcttct tcactgagtt tcacttgtat ggaacgtgcc ggaatatcga tttctatcat
    10501 gtcgccatcg cgcaataatg ctatattgcc tcctgctgcg gcttcaggcg aaatatgacc
    10561 tatagaaagc cccgaagttc cgccagaaaa ccttccgtcg gtaatcaatg cacattcttt
    10621 tcccaagtgg cgcgatttga tataggaagt cggatagagc atttcttgca ttcccggccc
    10681 tccttttggt ccttcaaaat tgatgactac cacatcacct gccacaactt ttccacttaa
    10741 aattccttcg caagctgcat cctgagaaga aaatactttt gccggaccgg agaatttcca
    10801 tatactttca tctacgccgg ccgttttaat aacacatcca tcctgagcca aatttccttt
    10861 caaaattgca agtccgccat cttttgtata agcatgatcc aaatcacgaa tacatccgtt
    10921 tgttctgtcg gtgtccaatt ccttataata agaattttga gaacccatta ccaaattaaa
    10981 ccggttggcc ggtgcacttt tatagagctg aatgatttca gccgaaacat ggggcgatgt
    11041 aatatcatat ttatgaatgg cttcctttaa agtcattcca tcaacgcgac gaacattggt
    11101 gtcaatgagt cctccacgac tgagttctgc cagtattccc aaaattccac ctgcacgatt
    11161 gacatcctga atgtggtatt tttgagtgtt tggtgctacc ttacataaac aaggtgtatg
    11221 gcgggaaagt tcatcaatat cgtccattgt aaaatttact tcgccttcgt gagcaacagc
    11281 cagcaagtgc aaaacggtat tggtagagcc tcccatggca atatccagtt tcatggcgtt
    11341 aagaaaagct tcacgtgttg ctatggaacg aggcagaaca gaagcatccc catcccgata
    11401 gtatttataa gcattttcaa cgatcagttt ggcagcttgt acaaacaatt tttcccggtt
    11461 gacgtgagta gctactattg ttccgttacc cggaagtgcc agtccaatag cttcggtcag
    11521 acagttcatc gaattggcgg taaacattcc tgaacaagaa ccacaagtag gacaggccgc
    11581 attttcaatt ctttgaattt gttcgtcgga aatcgagtcg tcggccgact gtaccattgc
    11641 atcaatcaaa tcgagctgta caccatccaa ttcgcctgct tccattggcc cacccgatac
    11701 aaaaacagta gggatattca atcgcatggc cgccattaac ataccgggag taattttatc
    11761 gcaattgctg atacatatca tggcatcagc cttgtgagca ttcatcatgt attccacact
    11821 gtcggcaatc aaatcgcggg aaggcagcga gtaaagcatt ccatcatgcc ccatggcaat
    11881 gccatcatca atggctatag tatcaaattc tgctgcaaaa catccaagtt tttctatttc
    11941 aacttttatt ttctgaccaa tttcgtgtaa gtgaacgtgt cccggaacaa actgagtaaa
    12001 agaattgact atcgcaatca cgggttttcc catttgttcg tttttcatgc cggcggctcg
    12061 ccacaaggct cgcgctccgg ccattcttct tcccgatgtg ctggttgtac ttctcaacct
    12121 gcttatcatt gattgttctt caacactgtt tattgaagtt cccattcaaa aattatttta
    12181 aaaatttata ttacttactg attttaaatc aattatttgt ttgcttttta cttttttcta
    12241 tgtaaaaaag taaattcaat aaaaacagaa aaacctttct ctatgaaggt gagaaaggtt
    12301 ttatctgtta tgctttatta tataaaaata ataagaacca tcctcacctt tgattgtgga
    12361 ccaaaagtag cacgacgatt cctaaaaaat tatttttccg atacatttct tcaaaattga
    12421 atttcggtgc aaagatagat gaaaaaatta ctttatcaaa aaaaatcata ttttttttca
    12481 atttgttata taaggcagtc ggaaataatt aaaaaataat tttatagctt tcaattattc
    12541 tgtgtataaa cttgtctttt ttataaaggc ggtataaaaa caaagcaata aattatttgt
    12601 ttaaaaagta gcaaacatta aaaaatgtat taattttgtg cacaatcata atctcagaaa
    12661 atggaaaata aaaactttac ctatatatct cacattgaat catctaagga tttgaaagtt
    12721 cttaaaaaag aagaacttaa agcggtttgc cgagaattaa gaacacttat tatcgacgaa
    12781 gtttcccaac atccgggtca tttggcatcc agtcttggtg ccgtggaact cacggttgct
    12841 ctacattatg tatttaatac tccctacgac cgcattgttt gggatgtagg gcatcaggcg
    12901 tatgcgcaca aaattcttac cggtcgtcgt gataaatttc acaccaatcg tgaatttaaa
    12961 ggaattagtg gcttcccttc tcccaaagaa agtgaatatg atgcttttgg agttggtcat
    13021 gcttcaactt ccatttcagc atcattggga atggatattg cttccaaact gaaaaatgaa
    13081 aaagacaggc atgtcgtagc cgtcattggt gatggagctc ttaccggcgg acttgctttt
    13141 gaagggttga acaatgtgtc aatgagtaaa aatatcaacg acttgttgat aattgttaac
    13201 gataatcaga tggctattga ccccctgaaa ggtggtatta ccgaatactt gattgatatt
    13261 actacttctc agacctacaa caaaataagg tacaaaattt ataaaatgct gaaagggttg
    13321 aatttgattg atgagtcgag aaaagacaat atcattcgtt tcaacaatgc gctgaaagct
    13381 acccttaccc gacagcacaa tctttttgaa ggattgaata ttcggtattt cggaccggtg
    13441 gatgggcacg atgttaatga attggtgaga attctctcag aaattaagga ttataagggg
    13501 cctaaaattt tgcattgtct aaccaaaaaa ggaaaaggtt acgagcctgc cgaaaaagct
    13561 gtaaccgaat ggcattcacc gggaaaattc tgtgtggaaa caggagaaaa aacttcttcc
    13621 aattctgttt tcgagcctcc tctttttcag catgttttcg gagaaaccct gctggaattg
    13681 gctaaaagca acgatagaat tgtaggggtt tcacctgcca tgctcagtgg ttgttccatg
    13741 aacatcatgc aaaaggaatt tccggaaaga gtatttgatg taggtatagc cgaaggccat
    13801 gccgtaactt tttcggccgg attggcgaaa gaaggaatga ttcctttctg taacatttat
    13861 tcgacttttt gccaacgagc ttatgacaat gtgattcacg atgtagcatt acaaaatttg
    13921 catgtagtga tttgtctcga tcgtgcggga ttggtgggtg ccgatggccc cactcatcac
    13981 ggtgtattcg atttggccta tatgcgttgt attcccaata tgaccattgc cgctcctcga
    14041 aatgagattg aattgcggaa tttgatgttt acggcgcaac aacccgactt gggaccgttt
    14101 gtaattcgct atcctcgtgg agccggatct gttgtcgatt ggagaaaacc aatgcagaga
    14161 cttgaagtag gtaaaggtga atgtttgaaa gagggtgaaa atttggctgt tttaactatt
    14221 ggaacaatgg cagtgaaagc cgaaaaagct attgcgaaag ttgaaaacga gtgccatttg
    14281 tcaattgcac attatgacat gcgttttctt aaacctttgg atgaaaatat gctccataaa
    14341 atcggagcaa aattttcata cgttttgact attgaggatg gcgttataca aggggggctt
    14401 ggcagtgcag tgctggaatt ttttgccgat cataattatt cggttcgggt gaaaagactt
    14461 ggcatacctg acaattttat tgaacaaggt tcgcctgacg aattatatgc catgctggat
    14521 atggacgaaa atgggatatc aaaaaccatt atcgacatgc tacaaaaaaa cactgaaaac
    14581 gataatccaa aaaaacaaca gatagtttct tccaataatt ttggaaaaaa taaaaattct
    14641 ataatcaaca gaattttcag aaaagatttc aaagaatgaa aattattatt gttggggcag
    14701 gagaagtcgg aacgcactta gcaaaattgc tggctcggga aagtatggaa ataaccctaa
    14761 gtgatgaaaa tcctgaccgg ctgatggatt tggaaagcaa ctacgattta ctcaccagtg
    14821 tgggttcttc cacttccatc aatcacttga gggaaattgg cgtagaaaat gccaatttgt
    14881 ttatagctgt tactccctat gaaagtgtaa acatgactgc ctgcatgttg gccaccaatt
    14941 tgggcgccga aaaaacgctt gcgcgtattg ataattacga atatcttttc cccgaaaaca
    15001 aggatttttt caagaaattg ggggtcgatt atttgattta ccctgaagtg cttgccgcaa
    15061 aagaaatttc ggaatcattg aaaaccagtt ggctacgaca aaatatgagt ttttgcaatg
    15121 atgctctgat tttgctgggt attaaggttc gaagcaattc ggaaattatc aacaaaaaat
    15181 tcagtaccgg ttattttgat catgacaagt acagagtagt cgccataaaa cgtgggatgc
    15241 agaccattat acccaaaggc agcgacgaaa ttttggacaa tgatattgtg tattttatca
    15301 ctacacctca atatttaaat ttcgtaaggg aacaagcagg caaggaagat tttcctatta
    15361 agaacgttat gtttttgggt ggcagccgaa ttgcacaaaa aacattaaaa agacttccgg
    15421 atcatattca taccaaagtt ttggaaataa ataatgaaag gagtatggag ttggctgaaa
    15481 agttcccgga tactctcatt atcaattgcg atggtcgaaa tattgatgtc ctgagagaag
    15541 aaggaattca ggaaatggat gcttttgtgg cagcaacagg aaattctgaa atgaatttgt
    15601 tggcatgtat ggttgccaaa cgttttggtg taaaaaaaac gattgcagaa gttgaaaaca
    15661 ttgattatat cccacttgca gaaagcatgg atataggcac ggttatcaat aaaaaaatga
    15721 tagctgccag ttatatttat caaatcactt taaatgcttc tgtcttgaat gtcagaaatt
    15781 taacttctgc cgatgctgaa gttgtggaat ttattgccaa agcaggttca aaaattacac
    15841 gttctaaaat aaagaattta aaactgcccg aaaatgtaaa tatcggtggt attgtgagaa
    15901 atggaaaagg aatgatagtc agtggagaga caagtataga acccgaagat catgtgatag
    15961 ttttttgtat ttcttcagct attcgtaaac tcgaaccctt tttcaacacc tgaccccatt
    16021 ttattcacta aaaccatgaa tcgtgaaata aattttaaac tggtgattcg tatgttgggc
    16081 agtcttctac tgagtgaagg aactgcttta ttgttggtgt tttttatttc attgatttac
    16141 ggggaaaatg atggtgttta ttttattttc acctctttgc tttgttatgt tgtcggcttt
    16201 gcgggtttct tttttggaaa aaatgctcct atgtctgttg gtaaacgcga aggttcgctg
    16261 gtggtaactt cggcatggat aattttttca ttgtttggat ctcttccctt ttggcttagt
    16321 aaaagcattc ctctttttac agatgccttt tttgaaacaa tttcgggatt tacaacaacc
    16381 ggcgcttcca ttttaaataa tattgaagaa ctgtcccact ccatgctgtt ttggagaagt
    16441 cttacccact ggatgggtgg tttgggcatc atcgttattt ctttggcttt gctgcctatg
    16501 tttggcacaa gcggggcaca actttttgca gccgaaagca gtggccctac gaaagataaa
    16561 attcatccca aaatcagtga aacagctaaa agattgtttg taatttattt tttgctcact
    16621 ttttccgaaa ctattttgtt gcgattggga ggaatgactt ggtttgatgc cgtttgccat
    16681 tcgtttgcca ccatagctac aggcggattt tcaaccaaac aaaacagtat ccaatattgg
    16741 gattcgcctt acattcaata caccataatt attttcatga ttttatccgg tgtcaatttc
    16801 agcctttatt atttcggatt taaaaacaaa ctgcagaaaa taagggaaaa tgaagaattg
    16861 cgttattatc tgattgtatt gtttggcttt gctattattg ttgcactttc catgattgac
    16921 ttttctcgac ccatatcttt tcacaaagtg gaagaatcat ggagaaatgc cttattcaca
    16981 gttggttcac ttatgtcaac cacaggcttt actactgttg attatatgca atggaaaccc
    17041 tttgtgtggg taattttgct gattgtcatg ctgacgggtg cttcggcagg ttctaccagt
    17101 ggggctatta aaatggtgcg tatcgtgatt gttttcaaat tttgttatta cgaattcaaa
    17161 agacttatcc acccaaaggc agtcgttccg gtgcgttaca gcggaaaaat tgtatttcag
    17221 gatgtggtta ccagagtgct ggtttttgtt ttgctttatt tgattgcagc cattctggga
    17281 atcttaattc tttgcatgtc gggtgttaat tttattgaat cggttggcgg acttgtggct
    17341 tgtttgggcg gcgttgggcc aggcttggga atggttgggc ctgtgggaaa ttattcttat
    17401 ttcccagttt ttgccaaatg gttcctgtcg tttatgatgt tgttgggacg tcttgaactt
    17461 ttcacggttt tgctgctttt tacaccagcg ttctggaaaa agtaattttt cgatgaaatt
    17521 aaaattttaa tctcagctgc ttttgttgta attttggtaa attcaaccaa agcaggtttt
    17581 attttattat tcaacaatgg aatcttttct ctatcgtatt gctcaaacat attacaacca
    17641 ctatgaagat aaaatttccg attttacctt tgtctttccc aacaggcgcg caggcatgtt
    17701 ttttcagcgc tacatttcgc aaatagccaa aaaaccgctt ttctcgcccg aaatattgac
    17761 cattaacgat tgttttacct ctgtagccca atttcatttg gctgacaggt tgagtctcct
    17821 gtttcgtctg tatagaattt ataaggaaaa aagtggtact aacgaaaatt tcgattcttt
    17881 tgcttattgg ggcgaaatgt tgctcaacga cttcgacgat atcgacaaat atcgcattga
    17941 tgcccgccaa ctgtttacca atgttactga actgaaacag attgaccgca tgttcaatta
    18001 tctttccgaa aatcagttgg atgctattcg tcagttttgg aaaaacttta tcccgattcc
    18061 taaggaaaaa acgcaggaag attttataca aatttggaaa attctttatc ctgtttatca
    18121 agattttacg gaggaattga agcgggaaaa catggcaacg gaaggtatga tttatcgcaa
    18181 cctagccgaa cgactcgaaa gcgaagaaaa aattcaagaa tgggaaggaa aacaatttgt
    18241 ttttatcgga ttcaatgcgc tgaatcgctg cgaaaaaact ctttttgaag tcctccaaaa
    18301 aaaagagaaa gccgattttt actgggatta tgaagctccg gctttacgcg atgccgataa
    18361 cccggcctca acttttttca tggaaaatac caaaactttt ccttccaaat tttccattga
    18421 aactcctgtc gaagaaagga aaaaacagta ttttgagctg atagccgtgc cttctgaaat
    18481 tggtcagatc aaacagattt atgaaattct aaatcgcatt tatcctcagg atgcatccgt
    18541 tcagcaagaa agttggatta aaacggctgt tgttttgcca aatgaaaatt tgctcttgcc
    18601 tgcgctttac tccctgcccg agcacatcga aaaggtaaat gttactatgg gttttccgct
    18661 caaaagcacc cctgtttccg ggttgattga gcatgttttc gacttgcaga aacgaattcg
    18721 caattccgat gaagaagtgt tgttttatca tcaaccggta accgaaattt tgcagcattc
    18781 ctacattacg ttgctttgtg gagacgacag caaaaatata cttcagcaaa tggtagaaaa
    18841 caactcgata tacattcggc ctgaacaact tcaaatcaat gagttgttta ctgctatttt
    18901 tcggccgcaa aacgatgcac gtgaaatgct gacgtatttg ttgaacattt tacgtatttt
    18961 acggaaaaat tttcagcaaa ttggttttgg aaatcatcaa tatcgaatgg aaaccgattt
    19021 tgtgattcaa tattatcgtt ttctaaaccg catgtccgat attttgcaat atcagtcaga
    19081 aggaatcgaa atgaagatgg atactttgat gcgattgatt tttcaatatt ctgcctttat
    19141 tactttacct tttgtcggtg aaccactgga aggtttgcaa attatgggcg tgttggaaac
    19201 caggggtttg gattttgata atattttgct gacttctttc aatgaaggtg tttttccaaa
    19261 aaatgctccc acccacagtt ttattcccta caatttgcgt cgtggtttcg gcttgcctgc
    19321 aaaggaacat caggatgcca ttagtgccta caatttttat aggctgattc atcatgccca
    19381 aaaggtttat tttttatata attccagtac tgaaggtttt cagacgggag aagtcagcag
    19441 gttcctttat cagctcgaat atctttacga cacaaaggtt gaacgtaaaa acattactta
    19501 cgatattttt ttgaaagatg cagtctccgc ttcggtagaa aaggactcac ttatccgcga
    19561 aaaattgcag cgttttgttt cttccggcga tgacaagttg tacctttctg ccagctccat
    19621 caatgcttac atagattgtc cgctgaaatt ttattatgct caaatagccc gaatcaaaga
    19681 aacggaagaa attactgaaa cagtggaaaa tgatttgttc ggtactttgt tccactcttc
    19741 tgctgaattg ctttacaaac catttaaaga tgtgcagctg ggaaaagaag attttgagaa
    19801 aattttgcgt cagccttcgt tggtggacga ggccatttca ctggcttttt caaaacattt
    19861 ttttaaaaac gaacaaaacg gaaaaatccc gccattagaa ggcaataatt tactcatcag
    19921 caaaatactg aaaaagtttc tgatgcgttt attgtatgtc gatcagcaat tgacaccttt
    19981 tcgttatctc ggcgctgagg aattttgcag tattcgcttc ccgctcgaaa cgctaaactt
    20041 gcgagtgaat ataaaaggct atatcgatcg gattgatgaa acggaaaatt ttttgcgtat
    20101 cattgattat aaaacgggaa cgggaaatac aacctttaaa ttgttggatg atttgtttga
    20161 tggttctctc ccgatgaaaa atcggccaaa acatattttt caggttttta tgtattgcct
    20221 gctttatcgt tccaaagccg aaaagaaaat tatttcaccc gccatttact atttgaagga
    20281 aatttttagc agcggttttt cttcttcaat tcaattcaaa ccggagaagc acactattca
    20341 ggtggaagat ttcggcattt tcgagcatga atttaaggaa aaactgactt tattgctgaa
    20401 tgaaattttt aatcctgaaa ttccttttaa gcaaacgcaa attaaagaaa actgcgacaa
    20461 ttgttctttt atggaaattt gcaatcgtta aaaattttct ttttcgacag ggaaaattgc
    20521 aaaagttgtt tcaaacctga gaagtcgagt atcaaaaatg agtagagaaa acatttttct
    20581 tcgaagttga gcatgaaaaa atttcaaaat cagaatttag tggaaaaatg tgctacagat
    20641 attttaaatc gacaaatatc ttttaaaaat gaaaattgaa acagaaagaa aatttttggt
    20701 aacgggagca tttaaatctc aagcttccaa aagttatcgc attattcagg gttacctgtg
    20761 ttctgatcct gagcgtacgg tcagagttcg tattatggat atttctgctt tccttactat
    20821 taaggggaaa agtaatcttt cgggattgag ccgcttcgag tgggaaaaag aaatccctgt
    20881 tgacgaggcg caggaattaa tgaaaatttg tgaacccggc attatcgaaa aaaagcgtta
    20941 cgaagttgtt tatcggggac agcgttttga agtggacgaa ttttttggcg aaaatgaagg
    21001 attgatttta gccgaaattg aattggaaaa tgaaagccaa gaggttttaa agcccgattg
    21061 gttgggagaa gaagttacag gaaaacctga atattacaac gccatgctca aaaaaaatcc
    21121 ttatcgatcg tggaaaaaat agaactttta gaaagtattt ttgttatatc aaattcatat
    21181 ttatccaaca gctacaacta aaatttcctt aaaccaaaac caaaaaataa tatgataaat
    21241 tgaaaaacaa tagtttgtaa aaatcatcag tttatgaaaa agtttttttc tttgttgagc
    21301 ctttttttgt tcgtgagtat attgtctcag gctcaaaacg agttgtttct gaaaaaatta
    21361 tttatttaca aaggagatac cttgccttat cgcattttat accccgaaaa ttacaacaag
    21421 tccgcttctt atccgcttgt tcttttcttg catggagctg gagaaagagg aaatgacaat
    21481 gaaaaacagc tggtgcatgg cgcttccctt tttactgatt cacagaaccg acaaaaatat
    21541 cccgccattg tagtttttcc gcaatgtcct gaaaatggtt tttgggcgcc aattaaagaa
    21601 cgggacaacg gattttcttt cataaattcc aaagagccaa caccatccat gcaaatggtt
    21661 attcggttac ttaaacaaat caggaaagaa gaggctgtag atagccgaag gatttatgtt
    21721 atgggacttt ccatgggagg aatgggaaca ttcgacctga tctgtcgtca gcccaaaacg
    21781 tttgcagcag ccattcctat ttgtggaggt gtaagtctgg aaaggttgaa gaaagttaaa
    21841 cacatgccca tcagaattta tcatggtggc agcgatgatg tggtttctcc agaacattcg
    21901 cgcaatgctt atattgaatt aaaagcgctt ggatcgacta aagttgaata tattgaattt
    21961 cccggagtaa aacataacag ttgggatttt gcttttgctc aacctgattt tttaagctgg
    22021 ttgttcagcc aaaagaaata actaaaaatc aattaattac attttaaaat ttttaaaaat
    22081 atttttcatt tgaagaattt ggataaaaaa attccaacaa ttttctcaaa tttcactact
    22141 tttgcgaaga aaaaaaattt aaaatataaa cagaatggct aaaataaaag gaactgtggt
    22201 cgtagatacg gaacgctgta aaggatgcaa tctgtgcgtc gtagcttgcc cacccaaagc
    22261 tttatcgctg tcgaaggagg caaattcaag aggatacaac tattctttcc tgagtaatcc
    22321 tgatgcatgt gttggttgcg ttgcttgcgc ctatgtttgt cccgatgctt gcatcacggt
    22381 ttataaaatg agagtacaat aaatttctac tcttcaaccg tatttattca atgtttaaat
    22441 tgtaaaaaaa tgaacaaaaa ctcaaaaaca gataacaaag aagaagtcct tctgatgaag
    22501 ggaaacgaag ccattgcaca tgcagccata cgttgtggat gcgatggata ttttggttat
    22561 cccattactc ctcagtcgga agttatggaa accttaatgg aactgcaacc ctggacaaca
    22621 accggtatgg tggtattaca ggccgaaagc gaagtagctt ccatcaacat ggtttatgga
    22681 gctgcaggtt gtggaaaaag agccatgact tcctcatcca gtcccggtgt cagtttaatg
    22741 caggaaggaa tttcctatat ggtgggtgca gagttaccct gcgtaattgt taacgtaatg
    22801 cgcggaggtc cgggtttggg aacgattcaa cccagtcagg gcgattattt tcaggcagta
    22861 aaaggtggtg gacacggtga ttataaactt ctaacgttag ctccggcttc cgtgcaggaa
    22921 atggccgatt ttatgccgct gtccttcgat ttggctttta agtacaggca tcccgtcatg
    22981 attctggccg atggaattat tggtcagatg atggaaaaag tggtgttgcc tcctttccaa
    23041 ccacgtcgca ccgacgatga aattcgtgaa caatgtccgt gggcgacgct gggtaagcca
    23101 tccaaccgac cacaaaatgt aatttcttcc ttggcgcttg aagctgaagc aatggaagcc
    23161 attaatctga gaatacagaa acgttatcgg gagatggaaa aaaatgaagt tcgttacgaa
    23221 acttatatgg ctgatgatgc cgattacctt attgtagctt ttggaacttg cgctcgcgtg
    23281 agtcaaaaag cggttgaaat ggcacgcgag aacggaatca aggccggttt gctgcgtccc
    23341 attacccttt tcccttttcc aaaagatatg attaatcaat tggcttcgtc ggtcaaggga
    23401 atgatttcta tcgaaatgaa tgccggacaa atggtggaag atgtccgtct ggcagttaat
    23461 ggaaaagttc ccgttgaatt ttttggtcga ttgggcggaa tagtaccttc acccgatgaa
    23521 gtttacgatg ctttggtatc caaactgatc aaataaaaat aattcttcag ccggttaatt
    23581 catttgctga agtttaatta acagtattat tcattcaaaa atttagaaaa atgtcgaaca
    23641 acgaaataat tagtcccgag aatttggttt atgcaaaacc caagtcactc aaagaaactt
    23701 atatgcacta ttgtccgggc tgcagtcatg gagtagttca ccggattatt gccgaaataa
    23761 ttgatgaaat ggacattcag gataaaacca taggaatagc tccggtagga tgtgccgtat
    23821 ttgcctacaa ttatattgac atcgactggc aggaagctgc tcacggacga gcaccggctt
    23881 tggcaactgc cattaaaaga ttattgcccg atcgttatgt gtttacctat caaggagatg
    23941 gcgatttagc tgctattgga acagccgaaa cgattcatgc ttgtaatcgt ggtgaaaata
    24001 ttgtgatcat tttcatcaat aacggtattt acgggatgac aggtggacaa atggcaccaa
    24061 ccacactgcc ggatatggta acttctacta ctccctatgg gcggaaaacg gaattgaatg
    24121 gttatcctct gaatatcacc aatttgttgg cacaattgcc cggaacttgc tatgttacgc
    24181 gtcaaagcgt tcagtcggta gccggagtgc gaaaagcaaa aaaagccatt cagaaagctt
    24241 ttgaaaattc catgttaaat aagggaactt ccatagtaga agtagtttct acctgtaacg
    24301 caggctggaa aatgacacct gaaaatgcca acaaatggat ggaagaaaat atgttccctg
    24361 cctatccgct gggagattta aaggacattt aatcgatgtt tcaatactct ttaaataccg
    24421 aatttctaca ctgatcataa attagaataa taacaataaa tcatattgat tttaaggtaa
    24481 acttatttgt tttaaaagag aaagtattat tgaaaaaaca ataaacagat gaaagaagaa
    24541 atcattattt caggttttgg tggacaaggc gtgctttcca tggggaaaat tctcgcatat
    24601 tcaggcttga tgcaaggcca ggaagtgagt tggatgccat catacgggcc tgaacaacga
    24661 gggggaacag caaatgttac ggttatttta agcgatgaaa aaatcagctc gccggttttg
    24721 agctcctatg atactgctat cattttaaat cagccttcac tggaaagatt tgaaagcaag
    24781 gtgaaaccgg gcggtacatt gatatttgac ggaaacggat ttcacaaatt ccctacgcgc
    24841 accgatatca acgtgtatcg aatcgatgca acagaatatg caacagctca taatgctgcc
    24901 aaaacgctca atatgattat tttgggagga tttttaaaag tcaggcccat tgtgaagata
    24961 gagaatgtgc tggaaggttt gaaaaaatcg ttgcccgaac gtcatcatca tttgattccg
    25021 caaaacgaaa aagctttgca aataggaatg gaattgatta aaaaagttaa ttgacttttt
    25081 tcctctggtt ttgaacatca atagactttt tttgaaaaaa agcgtctatt gatgttcttt
    25141 gttatttatt gtgtaacttt tgataaattg aaacaacaaa aaatttaata cttctcttca
    25201 aaaaaaatct cattttttct cccttttgaa aaaaaaatca ggttttaaga gtggcttctt
    25261 tttttataat ttatttaaac gctctgtatg ttactttaaa tattgtatct ttgcaagtgt
    25321 aaagctaatt ttttaaggtg aataatttta cagctgtttt aataactctt ttcttctccg
    25381 cttttttctc tgggatggag attgcttttg tttcttcaaa taagcttcga tttgagctgg
    25441 ataagaaaca gcgcagtata acttcatcca tacttactat tttttataag catccccagc
    25501 agtacatttc taccatgctt gttggaaata atatatctct tgttatctac gggttgctga
    25561 tggcaaaaat tttgtcccca ttgctacaaa tacttggtag tcaattttta attactttca
    25621 cacaaactat agtggctaca gctcttgttt tggttacggg agaattttta cccaaaagta
    25681 tttttcgtgt aaatccaaat ttttggttaa gcattttttc tcctttgtta ttgttcttct
    25741 atgtaatttt ataccccatt accttgttag caacgtctct ttcgtttgga attatgagac
    25801 taatgggtat aaaaatcaac cctttggcta atgaaaatgc cttctctcgc gtagatttga
    25861 attatttggt acacgaaagt tttgaaaaca ccgaacacaa gcaggatgtt gacaacgaag
    25921 taaaaatttt tcagaatgca cttgattttt caaaggttaa attacgcgat tgcttggttc
    25981 cacgtaccga aatagttgca ttggattaca atgtggattt ggaaacgtta aagcaaacct
    26041 tcattgaaac gggtttctct aaaattccta tttacaaaga aaatattgac aacatcattg
    26101 gttatattca ttcttctgaa atgtttcagc atcaaaatga ttggagagag catattaacc
    26161 agatacccat tgttcccgaa aacatggcag ctcaaaaact aatgaaaatt tttctgaaag
    26221 agaaaaaaag tatagcagtt gtggtggatg agtttggagg aacggcaggt attgttacat
    26281 tggaagatat tatggaagaa atttttggag aaatagaaga tgagcatgac acacatgatt
    26341 ttgttgcaga aaaggtcggc gaaaacgaat atgttttttc aggccgcctg gaagttaagg
    26401 aaattaatga aaaatttcat ctcaaccttc gcgaatcgga tgcttacgat actattgccg
    26461 gttttattct ttatcatcat cagcattttc ctaagttaaa cgagtccatc caaattgata
    26521 attatacttt caaatgtata aaaattacca ataatcgtat tgaactggtg aaaatgaaac
    26581 tacagaaata atttttttgt ctttttgaag tcaagaaatt caatcaaaaa tcaaagtatc
    26641 aagaattttt cgtatattcg tgcccttaaa attgaaaaaa taatagtttt atagcaaata
    26701 attttaaatg taaaatggca actttagaga agatcaggag caaaggagtt ttgcttattg
    26761 tagttatcgg acttgcttta ttggctttta ttgttggaga ttttcttaat tcgagctcca
    26821 ctttttttaa tcagtcgaaa gaaaaaattg ccgtcatagg tgatgaaaac atcagcatcc
    26881 acgagttcag tgcagccatt gaccagatga cggaagtgta taaaattgag acagggcaaa
    26941 cgaatttgaa cgaagaagtt gtatcccaat taagagcatc ggtgtgggaa aacatgctga
    27001 tggaaaaaat tttaactcac gaagcaaaaa aaatagggct ttccgtaagt cccgaagaac
    27061 tttctgaaag actgatcggg aaaaacatac atcctatcat tctacagcgc ggggctttta
    27121 tggatgaaac aggacaattt agtcgctctt tacttgtcca attcctcaac agtcttgatg
    27181 ttactcctac caataatgag atgaagcaac aaatagatca agctaaaaat tactggcttt
    27241 tttgggaaaa aaacgtaaaa aatgctattt tacaggaaaa atacaataag ttaattactt
    27301 ctgccatatc tgccaacagt atagatgcaa aaatgaattt tgaagctcga aaaattactg
    27361 ttgacgcggc ttatgtaatg caaccgtatt attcagtgcc tgattctatt tttaaagtaa
    27421 gttctaaaga acttagagac ctttataaca aacgaaaaga acttttcaaa caggaaaaat
    27481 ctgcatctat caattatgtt gccctggaaa tcaagccttc acaagaagat ttccaagaag
    27541 ctgaggaatg gattaacaag cttgaagacg aatttagaac aacagacgat gtggtaggtc
    27601 tggtaaatac caattccgac attatgtacg acggcagata ttattctgaa gcaactgtac
    27661 ctgccatgtt aaaagatttt gcatttagag ggaaaaaaaa tgatgtgttc ggacctgttt
    27721 ttgaaaacga cacttacact atggcgcgca tcatggaaac cgatattctt cgtcccgatt
    27781 cagttaagtt acggcatatt ttcctaactc aggaaaacga aggaaaagct gacagcatag
    27841 taaaagccct tcgagcgggt gccgattttg caactttggc cagaaagtat tctgctgttc
    27901 ctcaaactgc tgcaaacgga ggtgaaatag gctggttacc cgacggagct caaggagtgg
    27961 ataagcaaat tttggaaaac gcatttaaaa agcctctgaa tgaagttttt gtaataaaag
    28021 atattcaagg aactcaaatc atgcaggtaa ctgaccgcac gcccctacgc agaaaagtga
    28081 aacttgccat tcttcaaaga aaagttacac caagtagtaa aacatacagt caaatataca
    28141 accaagccaa acaatttgca gccgaattgg atgcagagaa atttgtttca agagcccaag
    28201 aaaaaggtta tattgtgcaa aaagccgaaa atctgaatga aaataccgaa caaataaaca
    28261 atttacccca atcccgtcaa atagttcgct gggcctttaa agggggaaaa ggcgatgttt
    28321 ctgatgtttt cgattgcggc aactattacg tagtggcgat gataaccgaa aaaaacgaaa
    28381 agggttatat gccgatagag aaagtttctg ctcagttgaa aagcgaaata ataagagata
    28441 aaaaggcggc gcaaatgata aataatattt cagagcaatt aaagaaaaca cctgacctcg
    28501 aagctttagc tgcttcactg aatgcagaaa ttaaaatggc cgaaaacgtt aattttactt
    28561 cttatcagtt tggattagcc ggatttgaac cttacatcat aggaaaagct acgattgttc
    28621 cactcgaaaa agtttcggga ccatttcaag gaaatgctgg agtttatgta atgaaaacat
    28681 caaacaaaaa ggagaatacc gaaacattca atgctcagca ggaaataatt caactaaatg
    28741 caagaatgct ttattctttg ccctatttga ttttagaaaa tttgagagaa aggactaaaa
    28801 ttgaagatta ccgttccaac ttttattgag gtattgagga gtttttcaat caatatttcg
    28861 ttaaaaaggt attgttaaat aaaaattgta aaaacgggat gaaactcttt cattccgttt
    28921 ttctttttta tggtcagttt ttttaacttt gcattggcaa caaatgtctg acaaaacgtt
    28981 tagataatta ttgttgttat aaaaacagat atttattatt ccctatctca ttaaaaatat
    29041 atttccctat gccaattcca cttttaggag cttcgttgga ggagcttacc gatattgttt
    29101 cacaactgga aatgcccact tacacggcaa aacaaatttc cgactggctt tataaaaaaa
    29161 gggtaactga tattaatgaa atgtcaaata tttccgttaa aaacagggac ctgttaaaag
    29221 aaaaatataa agttggccgt ttggctcctg ttaaagttac agtatcaaaa gatgaaacaa
    29281 aaaaatatct gtttcgaaca gaaaaagatc attttatcga aactgttttt atccctgaag
    29341 aacatcggaa taccttgtgt gtttcttctc aaattggatg taaaatgaat tgtgtgtttt
    29401 gtatgactgg gaaacaagga ttcatggggc aactttcggc atcggaaatt ctaaatcaga
    29461 tactttctgt tcctgaagct gaaaaaatta ccaatcttgt tttcatgggc atgggcgaac
    29521 cttttgacaa tacagtagaa ttattgcgta ccttaaatat tttaactgcc aattatggtt
    29581 atgcatggag cccctaccga attacggttt ccactatagg attaataccc ggtatgcaaa
    29641 cttttttgga aaaatccaat tgcaatttgg ctattagttt gcattctcct tttcatagcg
    29701 agaggctcga actaatgccc atagagaaag catatcccat ttcacaagtg gtagaagtgc
    29761 ttaaaaatta caattttgga cggcaaagaa aactttcttt cgagtatatc atgttcgata
    29821 agtttaatga cactatgcga catgcctcag aattggtaag acttttaaaa ggcatgaacg
    29881 cacgggtaaa tttaatccga tttcatgcca taccaaatgt ttctttaaga agttcaagcg
    29941 atgaacggat gatatggttt tgcgattatc tgaataataa tggaattttg gctaccattc
    30001 gtaaatccag aggagaagat atttcagctg cctgcggact gctttctacg ttggaaaata
    30061 aaaaaatgtc ataaccaatc tttcaaatga gaaaaataat caataaaaat aaatttcacc
    30121 tgatttctga aaaaacgatt ttgctattta tcattgtttt agcgggatta ttacctaaac
    30181 ccgtttttgc acaatcgtta ttagaaaaac aaatttcaga ctcgctaacc tctattgcga
    30241 ataaatatac atttgtggag aaaatatcgg tagataatat ttatatcaac aatagagaaa
    30301 aaaaaattat tatcaaagcc aatgaaaagt ttggttatat gccttttaga gaagaaaatg
    30361 taaaacatat ctaccgggta ctcaaacaaa tttttagtcc gtcctacaaa aactataaaa
    30421 tagtttgtat ggtcgacggt caaactatag aagaattaat tccaaatttt tatcgaacca
    30481 aaagaaaaag agacgaaacc agaatttttg atccaaaaaa atcgaagtat tcattggtaa
    30541 aaaatctttc aaaaccatat aatgtaacca acggattaga aagaaaaaat attgctctat
    30601 gggctagtca tggtttgtat tacgaacaaa gcatttctcg ttgggagtgg cagcgtgccc
    30661 gtgtgatgca aacagtggaa gatcttttta ccctatccat tgtttatccg tatctgatac
    30721 ctatgcttga aaatgccgga gccactgtat taacaccacg cgaaagagat atgcaaagca
    30781 atgaagttat tgtagacaat aacaataaag attcactctc atatcgggaa tttaacctac
    30841 aagaagtttg gacagaaagc caagattttg gatttgcaaa tttcaaaagt ttttatttac
    30901 aaggcgaaaa tccttttaac atgggaagtt accgtcaaat acattcggta gaaaaagaga
    30961 acaaagcaag ttttgttgaa tatatccccg attttcctaa agcagggaaa tatggcattt
    31021 atgtttctta taaaacacta ccccaaagta ctgaaaatgc tcactatacg gtctaccaca
    31081 agggaggtaa aaccaaattt tccgtgaatc agactatggg tggcggaact tggatttatc
    31141 taggacaatt taatttcgac aaaggaagaa atgaaaacgg caaagtggta ctatcgaatt
    31201 ttacagggga aaaagaagaa attgtaacag ccgatgctgt gaaatttggt ggaggaatgg
    31261 gaaatattgc ccgttctcca gatcagaatg gaatattgga aaaccataaa agttctgaaa
    31321 taaaaaatcc tgcattaaag aaagaaatgg tattgagttg ggagactgaa gccgaaataa
    31381 gcggttatcc tcgctttgca gaaggagcac ggtattggtt acaatgggct gggttccccg
    31441 acagcatcta cagccccaat cgcggagcaa acgattatat tgatgacttt cagtcaagag
    31501 ctaaatgggt aaattttctc acggcaggtt cttcgctcca tcccaaagga aaaggattga
    31561 agattccatt ggatttgtct ttggctcttc ataccgatgc cggaacaacc aaaaacgatt
    31621 ctgtaattgg cactttggga atattcacag tcggcaaggg taatgggaaa tcctataata
    31681 ataaagtatc gcgttgggcc tcgcgtgatt tgaccgatat tattcaaact caaattgtag
    31741 aagacatacg catgagcttt aatgatagtt ggataagacg cggactttgg aataaatctt
    31801 actatgaagc cagagttcct gaaattccga ctatgttgct cgaattactt tcgcatcaaa
    31861 attatgcaga catgaaatta ggactcgatc cccgttttca gtttttggta agccgctcca
    31921 tttacaaagg aattttaaaa tacctgtcgg cattgaatga cgaagaggat tatatggtgc
    31981 agcccttacc catacgacaa tttagctgca tttttgtaga taaaaacaaa gttaaacttc
    32041 ggtggaaaac aagaatggat tcattagaac ctagtgctgt agcagaacat tttgtggtgt
    32101 acacgagaat tgacgatggt gactttgata atggggtact tgtgtcggat accgaatacc
    32161 ttaaggtgtt ggaacaaggg aaaatataca gttttaaagt aacatccgta aatcgtggtg
    32221 gagagagttt tccttcagaa attttgtcgg tatgctctca tccgcacgaa aaaggcagcg
    32281 ttctcatcat aaatggtttt gaaagagtag ctccgcctgc cggttttgta atcgatacta
    32341 cctatgcagg ctttttgaac gacatggatg caggtgtccc ttatttatat ggctataatt
    32401 ttacgggaaa acaatatgaa tttagacgtg atagaaaatg ggtcgacgat gatgcaccgg
    32461 gtttcggagc tagtcatgcc aattatgaaa cccaaatact ggcaggaaat tcctttgatt
    32521 accctttttt gcatggcaaa gctatttgtg ccgccggtta ttcttttgtt tcatgcagtg
    32581 tggaagctgt tatgaatgac gatataaatt tgaacgattt tcagactgta gatttaattt
    32641 tgggaaaaca gaagcagact gatgttggca ctagagaaaa tatttcttta tttaaaactt
    32701 ttccattagc tttacagcaa aaaattaatt tgttttgtca tcagcaaggt ggaaatctac
    32761 tggttacagg ggcatcaatt gcgtctgata tgtttgacag aaatgacgcc ttaccagacg
    32821 atattcgttt tttagaaaac actcttaaaa tcaaatatcg ggtacatcaa gcggctatta
    32881 cggggaacgt aaaatttgta tcttctccat ttaaacaatt tgttcgacat caattttctt
    32941 attactccga acccaatcaa tattcttatt ttgtcgaatc acctgatgcc attgaacccg
    33001 ctgcaaggga tgcttatact ttttgtcgtt atgcagaaaa caatctgagt gcaggagtaa
    33061 tttattcggg gaaatataaa atttgtgcgt taggatttcc ttttgaaacc atcgtttcag
    33121 atgcagaaag agaagctttc atgaagtcag ttctggattt ttttgcaaaa ccatgaagac
    33181 aaataatgtc ccaaataaat aaaagatatc aaaaacagac caataaattt aagcgatttt
    33241 ttaaacttaa aaacaaaaga atatgaaacg agcattctta agatttcacg caagaatagt
    33301 agaacggatt cgcgtgagca atatttcaac tcttaaattt aattttattt tgaccaaaac
    33361 aaaaacaaca tttgttctga ctctactttt aatcatgtca ggttgcaatt ccaataaaag
    33421 ttccttgcca ggaattaccg gttcaatgta cgaagttttg gttgtaatgg atcatcaaca
    33481 gtggatttca gcaccaggtc gtacgttggt tgacttgttg gaccaagaca tggaagcgct
    33541 accgcaaccg gagcctgtaa tggatattat tccttgtcga ccggaagggt tctccaatat
    33601 gttcaagtct tcgagaaata ttattatttc cgatatttct gaaaaatact ctcaaccaaa
    33661 agtagtgtat gcacgtgata aatgggctca tccacaagcc attgtcagaa ttacggcacc
    33721 caatgacagt tcgtttgttg aagctgtgca aaaattcggg aaaaatattc ttgatttttt
    33781 tattactcag gaacgacaaa gacaaatgga atacaacaaa agttttgtga atttagaagc
    33841 tagagcagaa atagaaaaaa tttttggtat tcagatcgat attcctagcg aatttaaaaa
    33901 agcagttaaa aaaaaggatt tttactggat tacgaacgat aatccgaaaa taaggaaaga
    33961 tatagtaata tactcctatc catataccga caaaaacact tttacaaaag attacttatt
    34021 ggcaaaacgg gattcggtaa tgaaatataa tgttcaggga gaaatagaag gctcttatat
    34081 gggaaccgag tacaaatatt atccaccaat cttcagagaa atctgggtga atggtggtta
    34141 ttgtgcagag attagaggat tatggaaaat gtttcatggt gcagctatgg gaggtccatt
    34201 ctacagtcat acccgtttgg atgaaattaa tcagcgggta attactatcg aaggttttgt
    34261 ttttgctcct ggtgagaaaa aaaggaatgc cattcgtcag ctcgaatcag ttgtttactc
    34321 agctaaatta ccacaggaaa tcaatgcatt aaaggaaata tcggtaacac caaaaaaata
    34381 aattttcgat ttttacatta gaatatggaa caaaaaatta ttttaggaat cactcatgga
    34441 gattttaatg gaataggtta tgaagtgata ctcaaagcat tgtccgatcc tgttatcctc
    34501 gactttttta caccgattat ttatggttcg tccaaagctg ccggatatta tagaaaaatg
    34561 ctggatattc aaaatctgaa tctcaacatc attggttcgg taaaagaatt gaaccacaaa
    34621 agggttaata ttatcaattg tgtagatgaa aatattaagg tcgaagttgg acaatccaca
    34681 cccgaagccg gagaagctgc ctatattgcc ctcgaacgtg cggtagaaga tttgaaaaat
    34741 ggagaaatcc atgcgttggt tactgcaccc atcaacaaaa agaatatcca atcggaaaaa
    34801 tttcactttc ccggtcatac cgaatatctt gcccagcact tcgggaacac ggaaaatgcg
    34861 ctgatgttga tggtcaacga tgtgatgcgg atagctgttg taacggggca tattccacta
    34921 cgcgaggtcc cgtccgcttt aacttcggaa ttggtattga aaaaactgca aattctcaac
    34981 cactcactga aagaggactt tatggttgtt cgaccacgca tcgccatttt gggactaaat
    35041 ccacatgccg gcgatgatgg attgattggc gacgaagaaa aaactattct tctccctgcc
    35101 atgaaggaag ccgaaaaggc cggaataatc tgtgtcggtc cctatccaac tgacggtttc
    35161 ttcggatcgg gaagttttac aaagttcgac ggcattttgg ccatgtatca cgatcaaggc
    35221 ctgacacctt tcaaaacaat ttctatggac aatggggtga atttcactgc cggacttccc
    35281 attatccgca cttctcccgc tcatggcact gcttacgaca ttgccggaca gaatgttgct
    35341 tcagaaaatt ctttccgaca agctttgtat ctggcatgcg atgtttacaa aaacagggaa
    35401 cttaaccatc agctaaaatc aaatcagctc aaatttgaaa gcaaagaaaa agaaaacgga
    35461 taaataaaaa gtttttacgg aaacagataa gcgaaaatgg cgaatatcta ttgtagccga
    35521 atcgaagttc catttcatta atagaaaagg ggaaatgatt ttgatttgtt ttaaaacaaa
    35581 atgaaagtta caaaaaagtt aaaaattcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35701 nnnnnnnnna aaattcattt atttaagtgc ctttttgtcg gcttcattag gcctgttgct
    35761 gttttcgatt tgatgttcac aaaattattt caaattgttt ggtgagaaga agttgtttgt
    35821 ttgtattgta cactgtttgt tatcaaatag ctaattaaat ataaaagatt atgaaaatag
    35881 gagttcccaa agagataaaa ccgcaagaag gtcgggtggc aatggttccg gccggtgtta
    35941 tggacttggt ttgtcgatat ggccatgaag tttttgtaca aaagggagca ggagaagaga
    36001 gcggattttc caatgaagct tatttatcgg ctggagccgt tatgttggat accattgaag
    36061 aagtgtacaa catggctgat ataattgtga aagtgaagga accaattgaa agggaatatt
    36121 ctcttatcaa aaggaatcag atactgttta cttattttca ttttgcttcg agtaaaaaac
    36181 ttaccgaagc catgatgcaa agtggggctg tatgtattgc ttacgaaacc gtaatggaaa
    36241 aagatggaag tttgccgcta ttgatgccga tgagtgaagt agcagggcgc atgtctgttc
    36301 agcaaggcgc caaatatctc gaaaagccac aaaacgggaa gggaattttg ctgggaggcg
    36361 ttcccggtgt tcgtccagcc aatgtgctca ttattggtgg aggtgtagta ggaactcagg
    36421 ctgcatacat ggctgccggt ttgggagcaa acgttaccat gctcgatgtc aatctgtatc
    36481 ggcttcgtta tctcgacgat gtcatgcctg ccaatgtaat aactcaatac tcgagtaaat
    36541 atgccatttc cgaactttta cccacagccg atttggtgat tggtgcagca ctcacggttg
    36601 gagccaaagc gccgcatctc attacaagag aaatgctcaa acttatgcag aaaggaaccg
    36661 ttatggtgga tgtatctgtt gatcagggtg gttgttttga aaccactcat ccgactaccc
    36721 acaccgatcc tgtttacgaa gtggacggca tattgcatta ttgcgtcgcc aacattccgg
    36781 gtgcggttcc cataacttct acccgcgcac tgacaaatgc cacattgcct tacgttatca
    36841 agctggctga acttggttgg gagaaagctt gcaaaaatca cgaagatata aagcacggtt
    36901 taaacattgt cgatggcaaa attgttttta aaaatgtagc cgagtcattg gaattacctt
    36961 acaccgaata tcaaatttca taaaatggta cattggggat ttataggttg cggtgatgta
    37021 accgagaaaa aaagtggacc ggcttttcag aaagtcgagg gttcggatgt tgtggccgta
    37081 atgagccgtc gtctggacaa agccaaagac tacgctttac gtcatggagt gaaaaagtgg
    37141 tacgacgatg ccgacaagct gatacacgat ccagaagtta cggcagttta tgtggcaacg
    37201 cctccttctt ctcatgccga atatgccatc aaagccatga aagccggcaa acctgtttat
    37261 gtagaaaaac ctcttgcttc ttcctggctg gaaggtcaga aaattgttca agttgccgaa
    37321 gaaacccatg tgccttgttt tgttgcttat taccggcgaa cattacctta ttttttacgt
    37381 gttaaaaagt tgatcgacga gggaacgctt ggaaaaatct cttccgtaca gattcggttt
    37441 gctctgccac cgtcacaaaa tgattttcgt cccgatactt tgccttggcg agttaagaag
    37501 gaaattgccg gtggcggata tttttacgat ttggctccgc atcaattcga tttgctcgat
    37561 tttattcttg gtcctatttc ggaagcgcag ggttttacaa ccaacattgc cggactgtat
    37621 gaagtggaag atgcggtgag cggaattttt cgcttcagca acgggatttt gggtagcgga
    37681 atgtggactt tttctgctcc tgaaaatttt cgatgcgacg agattctttt tgtaggaaca
    37741 aaggcaacac tcacctgttc aactttcgat tttagtccaa tagtgcttga aaatggagaa
    37801 aaagtagaac aattttctga accaaaaccg gaaaacattc aattttattt gatagaaagt
    37861 attatcaatt ttttgaacgg gaaaggcact gaacctgtaa gcaattgcta ttcggccatt
    37921 cgaaccaatt gggtgatgga caccatattg aagaaaattt aaacttcaat taattttaaa
    37981 tcaagtaaaa attcataaga cacttattat ttttatcttg ctaagtcatt ttcgctttct
    38041 actaaaactt gagaaatttt ttctgttttt tgtaacatgt attaaatttt ttgatgatga
    38101 aaatttttca catatatttc acttacattt gtagaggtat taacgataaa aaatataagt
    38161 tatgcacaaa atattgttat tgatgtcggt aataatgact ctccacatga gcgttttttc
    38221 acaaaaaagc gaaccatcta ccatagtgca aagcgatggt ttgtttttca ccgatttgtc
    38281 ccaaaccaaa ctttatacag gggattatcg cgagttttac gacaatggat ctttgcggct
    38341 cgaaatgtac atcaaggacg gtaagccgga aggagattac attatttatt tccccaacgg
    38401 gaagcccaac gaaatacgtt cctaccggaa cggaaaattt cacggaacat ggcgcacata
    38461 caacgaatct ggagtactga tatctgaagc tttttaccgg aacgacaaaa aacatggtac
    38521 ttggcatata tgggatgata atggtatcat gcgctatgaa atgcattatc atgagggcga
    38581 aaaaatcgga acgtggttta tgtgggacga aaaagggaaa ttaatttccg aaaaaaacta
    38641 ttctacccct taattttttc atgtatttgt attttttttg taatactatt gtcatataca
    38701 gtaacttact ttgtatccaa acaaagggga ttaatttcaa acaaattaaa atacaaatca
    38761 aatgaaaaaa aatttttttg ctgtagtttt tttcgcattg tcggtttttc tcggaggcaa
    38821 tgttatggcc ggagagaata attcttcgga agcaaaaaat gtttcgaaca caaaaactgt
    38881 tatcgaaggt aaggtagtgg ataaaaatac ccttgaaaca ttggcaggcg taaaaataat
    38941 gctgaatggt caaaaagttt ataccgattt ggatggtcgt tttacaattt ctacatttcc
    39001 aaccgggaaa gctcgattga aagcaagttt gatttcatac gaagaacagg atgtggaaat
    39061 agatctgtca acatccaatt cacttcaaat taagttggca caacattgac atttttttta
    39121 aatctttttt gcaaaggcag ttctttttcg aattgccttt ttcaatatgt acattcccga
    39181 aaattcctgt ttcctctttt aaagtccaat atacgaatcg attgtatttt ccatcggtaa
    39241 aagtataatt atgccaaatt ctaaattcaa ctattttagt cagagaattc aatttttgtc
    39301 atatatttgt aatatatatt acataatatc gaaataattc ttcagttact ttgcaacatg
    39361 aataaaattt ttgctccaaa gtgagcataa attggttaat ttattatttc taaagtagaa
    39421 atttatgatt gctcccacac gtattttagt agtagatgat gaagaagatt tgtgtgaaat
    39481 tcttcaattt aatctggaaa cagaaggttt ccaagtagat acggcatatt ctgccgaaga
    39541 agctttaaaa aaggatttaa ctgtttataa ccttattttg ctcgatgtta tgatgggtga
    39601 aatctcgggt ttcaaaatgg ccagaatgtt gcacgataac ccgaaaacag cttctattcc
    39661 cattatattc ctgacagcaa aagacacgga aaacgataaa ataacaggat ttaatattgg
    39721 tgccgacgac tatatttcca aaccattttc tttacgggaa gttatagcaa gagtaaaagc
    39781 ggtgctgaga agatccgagt cgatgcaaat ggatgaaacc acaaataaaa ttcttacttt
    39841 tgaaaaactc gaaatgcatc tcaacaacaa aaaagtatcg ctggaaggaa atgaaattcc
    39901 atttacaaag aaagaatttg aaattttaaa attgcttctg gaaaacaaaa acagggtatt
    39961 ttcgcgtgaa gagattcttg caaaggtttg gacagacgat gtttgtgtgc ttgatcgcac
    40021 cattgatgta aatattaccc gattacgaaa aaaaatcgga ccttacggaa aaaatatcgt
    40081 tacccggttg ggttatggct attgttttga gggataaaca gatgatttta tttggtttta
    40141 gatgaggtgt ttttttaact cttttccttg caaattcgac gaccctgcat gaaactaaac
    40201 aaaaaaattt tcctatactt tttcagtata ttttttatcc tttttgtaat agttacctat
    40261 tttcagtatc atcgcgaaaa ggaattcagc agatcgcaac tggacggaca actgaacagc
    40321 tacgtgcata ccatccataa cttcattcaa agtgaacaac cgacttggga agaactcagc
    40381 agattcgttc gcctgtttcc tgacagcaca ctcagagtaa ccattgtcga ttctttgggt
    40441 aatgtaattt ttgattcctt tctgcccaaa ggaactcttt tccaaaatca tttggaccgc
    40501 ccggaaatag caaaagcccg caaggaaaag attggagagg ccatacgtta ttctgcttcc
    40561 acaggaaaaa actattacta tatggcccaa cgtttccctg aatattacat acgttgcgct
    40621 ctcccttata atgtcaatct cattgccgct cttagggcaa atatgttttt cttgtatttt
    40681 atgttattca tgtttgttat tacagttttt gcattgttta tcatttcgaa aaatttttca
    40741 cgctctatcg acagattacg aatatttact caaaaagccg aacaagacga tattctcgat
    40801 accgacattc agtttccgaa tgatgaatta ggcgaaatca gcaataatat tgttcaaatc
    40861 tatcgacgta tgctggaatc gaaagctgat gccgatcgtc aacgggaaaa actgtttaag
    40921 cacttgcaaa tctcacgcga gggattgggc attttctctt ccgacaaaaa agaaattctg
    40981 gcaaacacac attttattca atataccaac attctttccg atcaggaagt taaaagttct
    41041 gatcaaattt tttccatttc cgaattttcc gaaataaatg cttttattga cgattgcttt
    41101 tctaacaatg aaacaaaacg aaaacggatt acgatagaaa aaaatggaca ttctttttac
    41161 gttcagtgta ttgtatttca ggatgacacg tttgagatat ccataaatga tatttctgcc
    41221 caggaacatg aaaatgaact taaaaggcat cttacacaaa atatctccca tgaactgaaa
    41281 acgcctgtaa gtagcattat gggttatatg gaaagtattc ttgagaatcc caatatctca
    41341 cctcaacgtg aacgtttttt tatcgaacga gcttatcagc aaacccagag attaaatgct
    41401 ttgcttcagg atatatctac cctcaacaag ctcgacgaag catacaaatt gtttgcaaaa
    41461 gaaccgtgct ccctttctga agtcattgat gaagttctca atgacgtaca tgtccaaatc
    41521 gagcaaaaaa agtgcagaat tataaaaaat tatccttccg acatgaaaat aaacggtaat
    41581 aattctttac tgtactctat ttttcgcaat ttaatagaca atgctttgac ctatggtggt
    41641 gataatatta cggttgagat aaattgttac cgtgaagata acgagtttta ttatttctca
    41701 ttttcagaca atggacgtgg tgtagctgaa gagcatttaa acagactatt tgaaagattc
    41761 tatcgcgtgg acaaaggccg aagtagaaaa ttcggcggca cgggtttggg attggccatt
    41821 gtaaaaaatg cagttttatt tcacaaagga aatatctcag ctaaaaacca gccgggagga
    41881 ggtctaagtt tcatattttc tttaagaaaa ttctaaccca ctgattccta ataattttta
    41941 ttttctactg ctcgattttt ttgagggcta atatactgtt gcaaattttc ggttaaaata
    42001 gagtaaagta aactgtttta cgaatgtttg caggtgcgta ttttaatttt tttaatctaa
    42061 aatttgaatg ataaagaaat tgaaagaaaa aatcaggcta ttgcttgtaa atctgaacaa
    42121 attgttattt ttgtcaaaaa aatatttatg cagagagtcg ggtttctaaa aaatatttat
    42181 tcgttttatt ccgaaggttt taaaaacatg acggtgggaa aaaccttatg gataatcgtt
    42241 ttgataaaac tgttcatcat gtttggcatt ctgaaggttt ttttctttcc cgactatctt
    42301 aatgaacatt tcagggatga tgccggcaag gcgaaccatg tgcgcatgga acttatcaac
    42361 aaatcgaaat agaaagaatc aataaactga aatttatgga attattactg gatgcatcct
    42421 tagtaaattg gtcgcgggca cagtttgcca tgacagccat gtatcactgg ctgtttgtcc
    42481 cgctcacatt gggtttaggc atgattatgt cgattatgga aactctttat gtgaaaacgg
    42541 ggaaggaaga atggaaaaaa gcagcaaaat tctggatgac cattttcggg atcaactttg
    42601 caattggggt agcaaccggc attatcctcg aatttgaatt tggcaccaac tggtcgaact
    42661 acagctggtt tgtaggcgat atttttggag ctccgctggc catagaaggg atattggctt
    42721 tctttttgga agccacattt atttctatca tgttttttgg ttggaaaaaa gtggggaaaa
    42781 attttcattt ggcttctaca tggctaacca cactgggcgc aactatttcg gcattgtgga
    42841 ttcttgttgc caacgcttgg atgcaatatc ctacgggaat gacatttaat cccgatacgg
    42901 ttcgcaacga aatgattgat ttttggagta ttctcttttc tcctgttgcc attaacaaat
    42961 tttttcacac ggttatttcg ggctgggtat tgggtgcatt atttgttgtg ggtatcagct
    43021 gctggttttt gctcaataaa cgcgaaaagg atttcgcttt gagaagcatt cgcgtcagtg
    43081 ctttattcgg tttgatttct tctttgcttc tgctttatac cggagatggc tcggcttata
    43141 atgtggctca gcgtcagccg gtgaaactgg cagctatgga aggactttat gaagggaaaa
    43201 atggtgcagg cctgatagcc attggtattt tgaatcccga gaaaaaatca tacaacgaca
    43261 ctcttgctcc ttatctgttt aaagttgaaa tcccaaaact cctttcattt ctgggctatc
    43321 gcgatgccaa tgcttttgtg ccgggattaa aagatattat tgaaggtgga tatcaaactg
    43381 catcgggaga aacattatcg tttgaagaaa aaagagagcg cggtaaacag gccattcagg
    43441 cactggccga ttatcagcaa gcaagccaaa aaaaagatac ggttgcagcc aaaatgtata
    43501 aggacaggct catgaaaaac tatgcctatt ttggttatgg ttatctggac catcctcagc
    43561 aattgatccc gaatctgtcg ctggtatttt atccattcca tattatggtg attttgggaa
    43621 tttattttat tgtgcttttt gcgggggtac ttattttatc ttatcgaaaa aaattagagc
    43681 atttaagctg gttacaatgg atttgtttgt tgacgatacc attggggtat gtagccggag
    43741 aagccggttg gatggttgcc gaaattggtc gtcagccttg ggcgattcag gatattttgc
    43801 ccgttcaggt tgccgtgtcg agcatttcgg ccgaatcggt aagactgact ttcttccttt
    43861 tcctgatttt gtttacttct ttactcgttg ccgaaatcgg tattatggtc aaacaaataa
    43921 agaagggacc ggaagctgag aaaatagaag agtaattttt taggagaaaa ttcattttgt
    43981 ctttccttaa atttagaata ctatgataac ttattctttt ttacagcatt actggtggtt
    44041 tctgattgct ctgctgggag gtcttctatc ttttctattg tttgtacagg gtggtcaatc
    44101 catgattttt tcgctttcaa aaacagaaga tgaaaaacga cttctggtaa atgttctggg
    44161 acggaaatgg gaatatactt ttacgactct tgttacattt ggcggcgcca tttttgcttc
    44221 ttttccgctg ctttactcaa ccagttttgg tggtgcttac tgggtgtgga tgattattct
    44281 gttttgtttc accctgcagg cagtttctta tgaatttcaa accaaagcgg gaaatctgct
    44341 gggaaaaaat acgtatcgtt tttttctttt tctgaatgga ttattgggaa cttttttatt
    44401 gggcgtggtg attgccacgt tttttacggg ttcggatttt gtagtggaca aagcaaatat
    44461 ttccaatccg ctctcgccta ttatcagccg ttggcagcat ccgtggcacg gactggaagc
    44521 tttgctcaat ttgagaaatg tttgtctggg actggcggtt ttctttctgt cgcgcacact
    44581 ggcggctttg tattttatca atcgggtgga tcatgaaatg ctggaaaaac gttcacgaaa
    44641 atacttgtgg tacaatgcca ttccttttgt agttttcttc cttgcatttg ttatctggac
    44701 ttttctttcc gatggttttg ccgtacatcc tgtttcaggt gaagtatatt taagtcctca
    44761 ccaatatttt ctcaatctgg tggaaatgcc ggcagtgatg acaacatttt tactgggtat
    44821 agtgggtgtt ttgtatggaa ttattcgcac gctgttttca gaaaattaca gaaaaggaat
    44881 ttggtttgca ggagctggga ctgttctggc ggtgctggga cttctgttgc tggtgggctt
    44941 taataatact gcgttgtatc cttcagcaac acacgtgcaa agttcgctaa ccctttccaa
    45001 tgcttcttcc agtgaattta ccctgaaggt tatgtccatt gtttcgctga ttattccgtt
    45061 tgtggtaatt tatatttttt atgcttggcg ggcacttgag aaaaagcgta ccaatgtgga
    45121 ggaaatcaat gaagatccac atgcttattg agaaaaaggg gaaatagatt cggtaaattt
    45181 tcaattcgtt gaaatgaagt ttattttttg aacttcattt aattcgttga atgattgata
    45241 atccggagat tttattttta atacgacgtg gtcggaaggc tacgccgaat tatgggctat
    45301 acggaaaaga gaaacccgct tgaaaaaatg tgtcaatgta ttataatacg gagatgtagt
    45361 tttttcaaaa cttatttttg ttggaaatat ccttgaaatt atatgaattc cagataatta
    45421 tctttattta ccacataaaa ctttgcatga ggatatgtct cttgaaaggc ttttggaatt
    45481 ttcggaatct tgtttctcca tttgaactcc agtgctgtca gatcatttga actttcttca
    45541 attaaatcga tttcctgttt atcgtaagta cgccagaaat aaaactcttt gtgtaattct
    45601 tcattaagat ttgctttacg tctttcgctg atgagataat tttcccataa tgcacctaca
    45661 tcctgtcgga tagccagcgg cgagaatgct cctataatgg catttcggat accgttatca
    45721 taaaaatacc atttcccggc tttcgttact tccttgcgca gattacgaga ataagctcct
    45781 aaacgataaa taacaaatac tttttccaaa agatcaagat atttttcgac ggtcgttttg
    45841 ctcatgccta actgtttccc taattcgtcg taggaaacct cgttgcctaa ctgaaatgcg
    45901 atcagccgca acagttccct catcttacta gaatttttta ttccgtcgac agccaaaata
    45961 tcctttagta gataagctcc gacaatatca cgcaggtaat cggtcttgcg atcgaaatta
    46021 tccatcatta ctacttccgg atatgaaccg taaatcagcc gagcttcgag gttctgtcgt
    46081 gtttcaagca tggtttccat ttgcaatatt tcccgatgag aaaaaggtgt aagcaaaaat
    46141 tgcgaactgc gtccgaccaa tggttcaccc gttttgttga gcaggtcgaa tgacgaagaa
    46201 ccactcgcca aaacgcttat gccgggtatt tcatcaacaa tcaatttcaa aatgctgccg
    46261 atctgtggaa tgttctgagc ctcgtctata gctaccatat caattccatt aaacaaatgt
    46321 cggtagttgg ctatggtacg gttttccaac aatgtcagcg tatcgtagtc ttcgccgttg
    46381 agtaacattg ttttaccttg ataagactcc agaatttgat gcattaaaat cgttttccct
    46441 acacgccttg ctccgaaaat caacacgacc ttaccgggtg cgattttatc tattatctta
    46501 tcttgaagta tccttctaat aattttcata ctattttttt gttgcaaata taaaaaaagt
    46561 tattcaactg gcaatttttt cataaaaaaa agtattttca actggcgatt tttacaaata
    46621 acccataata aattgaatac caagttagat tttcaatacg gcagcacatt acttacaaag
    46681 cttaaatttg cggtaaacct tgaaaataat ggcaaatcct ttgtggcggg gattttttta
    46741 ttgagtcaat gttggttgtt tttcttcaat atcatgtcca tatctcctcc ataccttgtc
    46801 catgtttcct tcgtaccatg ttcttatctt ctccatattc agatcatact ttttatttga
    46861 acagaatgat gattttctct aactctataa aatgctttgt tcataatagt gccttttatt
    46921 gaaaattaat atgaaaccca catgtattgc atacacaaac acgaatgaat ataatattca
    46981 gacatgtggg ttccatcaga ccttaaaaaa ataaattata ttctgatgaa atgacatcat
    47041 tccttcaacc ttattttgct caaaccttaa taacaagaac actccttcca ctatccaaaa
    47101 ccttattagc atattaaaaa tctggaaatt ttgtttttac ttatgacgta gcctggaggc
    47161 tacgccgaac aaaaaggtaa aaatatgggg ctttgcccaa aaattccact tttatatgag
    47221 ccgttgcatg caacggctct actgaaagaa tggacgtttg ttgg
//
LOCUS       DAOSIB010000006        42520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_1056_length_42520_cov_12.003485, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000006 DAOSIB010000000
VERSION     DAOSIB010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 42520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42520
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1056_length_42520_cov_12.003485"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1748)
                     /gene="scpA"
                     /locus_tag="PLD44_01225"
     CDS             complement(<1..1748)
                     /gene="scpA"
                     /locus_tag="PLD44_01225"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOF97992.1"
                     /translation="MLQTEENWLTPEQIPVKPFYEKNDWKGMEHLHYAAGIPPFLRGP
                     YSSMYVTRPWTIRQYAGFSTAEESNAFYRRNLAAGQKGLSVAFDLPTHRGYDADHERV
                     VGDVGKAGVSICSVEDMKILFDGIPLNKMSVSMTMNGAVLPILAFYIVAGLEQGAKLE
                     ELSGTIQNDILKEFMVRNTYIYPPQFSMRIIADIFKYTSQNMPKFNSISISGYHMQEA
                     GATADLELAYTLADGLEYLRTGINAGLDIDAFAPRLSFFWGIGMNHFMEIAKMRAARL
                     LWAKIVKQFHPKNPKSMALRTHSQTSGWSLTEQDPFNNVGRTCIEAMAAALGHTQSLH
                     TNALDEAIALPTDFSARIARNTQIYLQEETNICRQIDPWAGSYYVESLTKELAEKAWD
                     LIQEIENLGGMAKAIETGIPKMKIEEAAARTQARIDSGKQIIVGVNRYTLEKEEPIEI
                     LEIDNTAVRMQQIERLKQLRASRNNEEVKKALDAITECARTGNGNLLELAVEAARVRA
                     TLGEISDACEKVAGRYKAIIRTISNVYSSETKNDANFQRASQLTEEFAKKEGRRPRIM
                     IAKMGQDGHDRGAKVVA"
     gene            complement(1980..2600)
                     /locus_tag="PLD44_01230"
     CDS             complement(1980..2600)
                     /locus_tag="PLD44_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOF97993.1"
                     /translation="MVMKRIIIPILIAIIFWFVMFSPWTKEYVNFWLTMTAAGITLTL
                     MSAFLGKDFKKQFSLSLKDVLIGLGSAVVLYVVFFLGDFFSKLLFDFAKDQVGQIYMM
                     KEGENPLLLSLLLILLIGPAEEIFWRGYVQRMLEPKFGSWMALIVTTLIYTLVHIWSF
                     NFMLIMSAMVCGAFWGLLYKYNKNLVTLIVSHAVWDVSVFILFPIV"
     gene            complement(2591..3493)
                     /locus_tag="PLD44_01235"
     CDS             complement(2591..3493)
                     /locus_tag="PLD44_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOF97994.1"
                     /translation="MKEKIKNGLLATRPWSFPASTMPALLTISYVFFLKNELGSEVNW
                     WFGVLALLGAAIFQASGNLIGDYFDYQHHVDRKETFGSSRMLVDQVFQPKTILHFGIV
                     LLAVGVVLGLFLCYRTGIDLLWIGAIGAMATYFYYKLKFAALGDLTIFIIYGPLIGLG
                     TAYVMTGQLMWFAVVLNIPVAFLVVNILHANNTRDIRDDGKANIKTQAMLLGIKGAKI
                     QYVVLSIGAYVGVVLMIIFKMIHPVALLMLLSLPLAVKNIKKMMTAEVEKPEKIFDLD
                     AQTAQLVLVFSLLFSISNFIAAWL"
     gene            complement(3570..5465)
                     /gene="mutA"
                     /locus_tag="PLD44_01240"
     CDS             complement(3570..5465)
                     /gene="mutA"
                     /locus_tag="PLD44_01240"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase small subunit"
                     /protein_id="HOF97995.1"
                     /translation="MAEKKLNLLADFPPVSTQEWLDKIIADLKGADFEKKMIWRTNEG
                     FNVRPFYREEDVDDLKITELLPGAFPYVRGTKSDNEWFVRQDIKVENAQEANAKALYI
                     LNRGVNSIGFQLKKADLSKEFVATLLKDIAADCVELNFTICISKAAELADILSEYFIA
                     QNYEVKKLQGSINFDPINRMLLAGKKLTKEEIAEKAKAVVQAISRLPFYRVIGVNATT
                     LSNAGSTITQELGYALAWGNEYLSMLIDAGVDKAVAAKKIKFNFGIGSNYFMEIAKFR
                     AARMLWALIVDAYQPPCPRPDCPNTVNGICKCAAKMRIHAETTIFNQTIYDAYVNMLR
                     TQTEAMSATIGGVDSLSVLGYDTAFKPSDEFSERIARNQQLLLKEEAHFSEVVDPAAG
                     SYYIETLTAEIANQAWKLFLNVENEGGFLAAVQNGKVQQTIKATANERLKAISNRKEI
                     LVGTNQYPNLLETAAEKIDKELQQCGCCSHEEGTEKLLPLRGAVEFENLRLAVEKTSK
                     RPKVFMLTVGNLAMRSARAQFSGNFFGCAGYEIIDNLGFSSVEEGVKAALDAKAEIVV
                     LCSSDEEYATLAPEAYKLLKDKTIFVVAGAPACMEDLKKEGIENFISIKSNVLETLKD
                     FNKKLGL"
     gene            5763..6989
                     /locus_tag="PLD44_01245"
     CDS             5763..6989
                     /locus_tag="PLD44_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOF97996.1"
                     /translation="MQKLIIFIFSVLFAFPLYAKRVMTGIEVLRADNFKLLEGKRVGL
                     ITNPTGVDGNLKSTIDILYEARNVKLVALYGPEHGVRGDVYAGDKVVNATDEKTGLPV
                     YSLYGTTRKPTSEMLKGIDVLVYDIQDVGCRSYTYISTMGLAMEAAAENDIEFIVLDR
                     PNPLGGEKIEGNLTEEPFISFVSQFKIPYIYGQTCGELALMLNEENALKKKCKLTVVK
                     MKNWKRSMKWEDTGLEWVIASPHIPHKHSAYYYPVTGILGELGYVSVGVGYTLPFQVV
                     AAPWIKAQEFADAMNRLHLPGFIFRPIFFTPFYGTLKGENCQGVQIHIVDFKKAHLSL
                     VQFYIMQELARLYPDKKVFDNANKERFSMFDKVSGTDQMRKLFQVNNSVSDIQHYWDK
                     DIQSYYQLSKKYYLYH"
     gene            6999..9833
                     /locus_tag="PLD44_01250"
     CDS             6999..9833
                     /locus_tag="PLD44_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HOF97997.1"
                     /translation="MINSFLSEIKSFIPEKRIYTDELRLLAWGTDAGFYRLIPKAVVQ
                     SASEMEVSLLLRAATKHHLPVTFRAAGTSLSGQAISDSVLILAGSNWERYELAPDASF
                     IRLQPGIVGERVNQILKPFRKKFPPDPASIKSAMVGGIIVNNASGMSCGIRENSYRML
                     ISTRIVLPDGSVLDTGDEESKAVFAKTHPEFLRKIEDLRDEIRNDVTLKQLIEKKYRI
                     KNVTGLSINSFIDFDDPFDIITHLMVGSEGTLAFLSEAVMKTTTDYPYKASAMAYFEN
                     MHLACETVKNLKSFPVSAVEFFDRKAIRSVEDKFETISELKTLPDNSAALLIKIEASD
                     GNELNERIRQVEKVLNDAHTLLPVSFTDKESEYKKYWDMRSGIFPSVGSTRPVGTTCL
                     IEDIAFCIEDLAEATDDLHEIFIKNHYEDAVIYGHALDGNLHFIISQRFDTEEYIHQY
                     EKMMNEVIEMVVKKYHGSLKAEHGTGRNMAPFVKKEWGEKAFDIMLQVKQLFDPEGIL
                     NPGVIFNDNPRCHLENIKPMPAVHPLIDKCIECGFCEVNCVSAGFTMSSRQRIVVQRE
                     IKRLKTSDENSRRLKELEKSYRFMGEQSCAGDGLCATSCPVSINVGDYIHVLRNENMK
                     NKTMAQFLGRWTAKHFNWVASGLKSVLYIASAAQHILGNKAMGAITRGIHKWSGGVFP
                     LWTPAMPKRRNKIEVSTSTSTDSSLKVVYFPSCLNQMMGVSKNDPELTPLVEKTVSLL
                     HKAGYQVVFPKNMEKMCCGSIWESKGLLSVDEQKSSELEKALREASENGKIPILCDQS
                     PCLYHMRKSMKQLSLYEPVEFIHDFLLDKLDFYPIDEPITIHATCSTIKMGLQPKLVE
                     IAKLCSTQVLVPEEVGCCGFAGDKGFTMPELNAHALRKLRPQIEKAHVKVGYSNSRTC
                     EIGLNTNTGIPYQSIVYLVDKCTKNKINRS"
     gene            9854..11371
                     /locus_tag="PLD44_01255"
     CDS             9854..11371
                     /locus_tag="PLD44_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOF97998.1"
                     /translation="MRKFRLSVVLLFFAFSFLQAQPKREMRAVWIATVANIDWPSSNR
                     LLPTQQRIEIVNMLDRLTENNINAIVLQIRPMADAFYPSNLEPWSHWLTGRQGVRPSP
                     FYDPLQFIIEEAHKRCMEVHVWLNPYRVTTTGDSMMLSKEHLFYKQPELFVRYGGKYY
                     FNPGLDETRNFLNKVVEDIVEHYDVDGVHFDDYFYPYPVKGEDFPDDETFMKFPRGFA
                     PNQKADWRRNNVNMAIAEVQQTIKNLKPWVEFGVSPFGVWRNDNVDPRGSATRAGIQN
                     YDDLYADILKWLAEGTIDYVTPQLYWEIGKKVANYEVLLKWWIENSYGKNLYIGLIPS
                     SLGDKKNPAWTTGNEIIRQIKMNRNSSQVDGAVFFSAKTFLQNRQGLNDSLQSNYYRY
                     PALCPINRNILGEPSAQPQNLRVLRDGKDAFLLWDEVNEEGGCQTAYYVVYAFKGKEV
                     GDLNDPVNIIARTTENCIDLRILPHKFKGNYTFVVTAVNRFRQESIPTQGITRKL"
     gene            complement(11396..12820)
                     /gene="cls"
                     /locus_tag="PLD44_01260"
     CDS             complement(11396..12820)
                     /gene="cls"
                     /locus_tag="PLD44_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOF97999.1"
                     /translation="MSEIISIFFIALYVYTILTTISVLLLENRNPSKSLSWMLIILFL
                     PVIGLILYLIFGQDLRKQKIISRKSIHSLDHRDISSFDFSKINSDLLDAHQMNLIKLL
                     YRNSEAVGYTYNKIEVFSDGKEAFESIFQAVEQAREHIHLEFYIISDDSITNRLKDLL
                     IKKSHEGVRIRIIYDFIGSFNLSRKFLKTLTKAGIYVRPFFPLRFRISRSRINYRNHR
                     KLLVVDGKVAFTGGMNIADRYVYGNRLGYWRDTFLKIQGSAVHGLQHLFLSDWYFVEK
                     KLITDKKYYPVPAQFDNNLVQIVSSGPDTEWEAIMQGIAKAIMSAKKYIYIQSPYYMP
                     TELIEGCLQLAALSGIDVRIMIPTQSDSPIVIASTSSYLGKVLQAGVRVFYYRKGFLH
                     AKTVVMDDFVSIVGSCNIDYRSFYQNFEANAFVYEEKTALTLKELFLEDIHYCEEITL
                     EQWNNRKKTKKLMDSVSRLFSPLL"
     gene            complement(12821..13876)
                     /gene="ribD"
                     /locus_tag="PLD44_01265"
     CDS             complement(12821..13876)
                     /gene="ribD"
                     /locus_tag="PLD44_01265"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOF98000.1"
                     /translation="MDIEIKYMQRCLQLASLGDGYTAPNPLVGAVVVYNDTIIGEGFH
                     QHFGQAHAERNAIFSVKQPELLKDATLYVNLEPCSHYGNTPPCASLIVEKGIPRVVIG
                     TIDPNPKVSGKGIQILKDAGIETYVGVLEEKCRELNKRFFTFQEQKRPFIVLKWAQTI
                     DGFIDRIRNDVSEPPLIISNEITKQLTHKMRSENQSIMVSTNTVLLDNPSLTVRNWTG
                     KTPIRITLDRQGRIPENYHLLDGLVPTLVFTERQPAKLNNVEYIQIDFKKEGLRGILK
                     EIFDRNIHSVLVEGGAQLLNSFIAENLWDEANVEVAPIEIGNGIAAPVMTCQPASRKN
                     FDGHEWLHFYNPKKTIK"
     gene            13966..14805
                     /gene="prmC"
                     /locus_tag="PLD44_01270"
     CDS             13966..14805
                     /gene="prmC"
                     /locus_tag="PLD44_01270"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOF98001.1"
                     /translation="MENILQYIHSELKGIYPESEIRFIGYLLLEKLTGFSRTEILLNK
                     NTQISCEAAKQLDGFLKKLKNHEPVQYVLGATEFCGLSFNVNPSVLIPRPETEELVEW
                     IYSENDRNKPLKMLDIGTGSGCIAISLKKNFPLAEIDAFDVSPEALKVAKENAKLNRV
                     NVNFREIDIFNPPAFSKKWDLIVSNPPYVPENEKSEMQRNVTDYEPSLALFVPDNHPL
                     KFYETITNFARLNLAKGGKLYFEIHYRSGKNMLDLLSQNGFREVQLKKDLSGKDRMIS
                     GIF"
     gene            complement(14987..15625)
                     /locus_tag="PLD44_01275"
     CDS             complement(14987..15625)
                     /locus_tag="PLD44_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOF98002.1"
                     /translation="MNQRFVINIGRQLGSGGHLIGQGIARELGISFYDKELIQIASQE
                     SGLAKEFLEQLDEKKGQGFFRGMFGLRNSIMIDEYYFNDNYPSSEMLFKIQSDVIRSL
                     AEKQSCVFVGRCADYVLKDHPRAVNIFITADLPDRIARVCRNQKMTEEKAMDYIKKMD
                     KKRADYYNYYTDKQWGAAASYHLCINSSALGIEETISYIIEFVEKKLHFDKK"
     gene            complement(16016..17146)
                     /locus_tag="PLD44_01280"
     CDS             complement(16016..17146)
                     /locus_tag="PLD44_01280"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HOF98003.1"
                     /translation="MKKIFLYLATVLMAGCSSSIKTIEISNTGSFDRNDEIIQINDKE
                     FLQLFEESDKWILKNNHNQEIGYQLVKNENGNLETFIFQAAVPANKKVAYHLKKGNPS
                     PVEPKTFARFVPERKDDFAWENDLAAYRMYGPALAGENPSNGVDLWLKRTKELIVDTF
                     YFNELQKGQSYHIDHGKGLDCYKVGHTLGAGGIAPFWNDSLWIGNYFNSYKVHYVGPL
                     QSKFSLFYDSIKVGDKFYKEQLTITVSAGTLLNKAEVTFNGYQQSLPLAAGIFLHENT
                     GKLSMNQEEGTAAYCEDAVSDAKVPSGRNYVGIFVPAPITKAIIKNNHALLISEYQPG
                     NTFVYFFGGGWNKWGFPTDNDWINAVNLFAQSYRSPLEVLIK"
     gene            complement(17221..18000)
                     /locus_tag="PLD44_01285"
     CDS             complement(17221..18000)
                     /locus_tag="PLD44_01285"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HOF98004.1"
                     /translation="MNQFDLKGKIALITGASYGIGYAIAKAFHAAGAVIVFNDINQEK
                     VDMGLASYKADGIDAHGYVFDVTDEEAVTEAIAKITKEVGTIDILVNNAGIIKRIPAL
                     EMSAKDFKQVVDIDLNGPFIVSKAVAPGMIAKGGGKIINICSMMSELGRETVSAYAAA
                     KGGLKMLTKNLACEWGAFNIQVNGIGPGYISTPQTAPLREEGHPFNTFIVTRTPAGRW
                     GTPEDLQGPAVFLASSASDFVNGHILYVDGGILAYLGKQPS"
     gene            complement(18080..18922)
                     /gene="kduI"
                     /locus_tag="PLD44_01290"
     CDS             complement(18080..18922)
                     /gene="kduI"
                     /locus_tag="PLD44_01290"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HOF98005.1"
                     /translation="MKTNYEIRYASHPQDAKNYDTSRLRKEFLVERIFTINEVNMVYS
                     LFDRMLVGGAMPVAEVLTLESIDPLKSDFFLERREIGIFNVGGKGCINVDGTNYELDY
                     KEALYIGRGNKKVTFESVDGSNPAKFYFNSATAHKACPTRKITKENAVILEMGSLEGS
                     NHRFINRMMVSQVLETCQLQMGMTELEPGSVWNTMPAHTHSRRMEAYFYFELPDDQAV
                     CHFMGEPEETRHIWMKNDQAVISPQWSIHSAAATSNYTFIWGMAGENLDYDDQDFYKK
                     TDLK"
     gene            complement(19207..19836)
                     /locus_tag="PLD44_01295"
     CDS             complement(19207..19836)
                     /locus_tag="PLD44_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOF98006.1"
                     /translation="MRKISIFLCIFLFSANLFAQPSSEKPEIYFGTTQGISASMINFS
                     PSVNQGMLLGYNGGLIFRHITEKNVGLQVELNFFQRGWKETDSIYSRRLNYIELPFMT
                     HIYFGNKSRFFFNIGPKISYLLTENVLKNETVSSEKVQHVKSVENPFDYGICGGLGFM
                     FRIRKNVLQLDARANYSLSDIFSNNKTDFFDSSNNINFSVNLGWLWQVK"
     gene            complement(19841..21778)
                     /locus_tag="PLD44_01300"
     CDS             complement(19841..21778)
                     /locus_tag="PLD44_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOF98007.1"
                     /translation="MKKNIFCLLTVIILSLTGFSMEQTNSYPIKKVNGIEYYVYTVQS
                     GDGLYAISRKFGIAPEEISKVNPEIQKGLKSGQKILIPIQKNIQENEDASQRLKNELS
                     LLQEKSFEENQKNLHQQTDNSLPSTTLFLQHKVEKNQTLFGISKKYNVSEEEIIQYNP
                     QSKNILREGDVLFIPYKTQTTKNETEIKGDLVEVITPSGKKELNEKQLPSKDKPQSIK
                     HLVKPNETLFSISRTYNVTIDDILKLNPDAFPVLKIGTELTIPTSILKKDSIPSTNLN
                     NNEMDSALQDIENPEIATAISTENNIKIAFLLPFNLDTPSQQNANGRFRDFYAGSLLA
                     IKKAKENNYSLEIFTFDTEKSDEKLKEILAKPEMKSVDLIVGPAYSNQISLISDFAKE
                     NKINVLIPFSSKVYEIGINPYLFQFNPNDTQIEIDFLVDKIIQKTYRNANIVFVNLPG
                     ISILDEGNLFMERLKNRLLKERHKFNEFNFSSSNKNQSIPEVLKRNQKNLLFFNTDNS
                     SLITPFLKEFYVYIDEFDMTLYQEYGWENQYIERFDRIYVSPFKPEIDASALEEYQKT
                     FHQFFEWEVESERPRYDLLGYDLLNCFIPILKNYENNLTKKLKEITFDEGIQSDFHFK
                     KQSEFSGYINDKLYMGERKAK"
     gene            21975..23222
                     /locus_tag="PLD44_01305"
     CDS             21975..23222
                     /locus_tag="PLD44_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOF98008.1"
                     /translation="MKKLTISFCFYLIALSVSAQLVATDKYAVYQNFTGVDYLFVFNG
                     ILPGTEIRFTGSGDNVKWYRFSDTTTPYSTLPYIFPEDASGYILDVDGHQTSVWVIDY
                     QNYLPVFHTLASDESSDNRCNELTLLLDANVPELYYYTLSGIRRKINRNFTLSYQTLE
                     WDNEWKTVETTKNVTLPVTEITVPAPLCNTTFSLQGDQFTSEPSLGIQPIGIISSMYA
                     AVSLECHIKTSTTARTEKNEDERPTSDALSGSAPLEMLFNAEVNNAGTIFYKWDIYKD
                     NQLLVSRSDKEHRYTFTEAGTYNVKLTVNNDFCSSSDSVSISVSTSDLQVPNVFTPNG
                     DGINDEFRVAYKSLISFDARIYNRWGRMVYHWNDPQKGWDGTIGGKKAPVGAYFYVIK
                     AVGADGIKYSKKGDINLLRGREN"
     gene            complement(23295..24062)
                     /gene="ubiE"
                     /locus_tag="PLD44_01310"
     CDS             complement(23295..24062)
                     /gene="ubiE"
                     /locus_tag="PLD44_01310"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOF98009.1"
                     /translation="MNKEKTTSQYTFQEVKPYNEKEGKTEQLVRMFDTIAAQYDKFND
                     LMSWGFAKLWRKRALSTLKRFRPKHILDVAAGTADMCIKAFRLLNPDKVTGIDISPKM
                     LEIGKEKIEHAQLSDKIKLKVMDCAQLNFDDNSFDAVTIAFGVRNFEKLEESLHQIFR
                     VLKPGGHLLILELNEPQKGWLMKGYQLYIKLFVHQTAKYLSSDQNAYRYLTSSMHAFP
                     KGKEMIEILQNNGFELVEYKKFTFGVCSMYLMEKPTH"
     regulatory      24244..24463
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            24629..25357
                     /locus_tag="PLD44_01315"
     CDS             24629..25357
                     /locus_tag="PLD44_01315"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HOF98010.1"
                     /translation="MKKKSLIVLSTLLLIFFTSCENDSDFKLKTTVDFEDVELGENGY
                     WNGSDFSKKFVSNGFIFLNDFTSSPWGDFWSGFACSSLTDTITAGYDNQYSVAAGSGA
                     WGSNKFAVAFDDSATVNCPKNELGTYEAKSIMLCNSTYAYLDMRNGSAFSKKFGDGDW
                     FKVTIKGFLNNNATGAVDYYLADFRNGKHYLCHQWVKVDLSSLGKVDQLIFLFDSSDK
                     GTFGVNTPKYVCIDNLEFEQDAKN"
     gene            25712..26296
                     /locus_tag="PLD44_01320"
     CDS             25712..26296
                     /locus_tag="PLD44_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HOF98011.1"
                     /translation="MEYTTLQGKLIMPEYGRNIQQMIAYAMTIEDREERTRCVKTIIN
                     IMGNLFPYLRDVNDFKHKLWDHVAIMSDFKLDIDFPYEVLRPENLYTRPEKIPYKNSD
                     IRYRHYGRTLEEMIEKVADYPDGEEKNELIRLIANQMKKCFLTWNKEVVDDRKIFDDL
                     RELSKEKLDISEDFLKLVESKEVLHNKKNKKKNK"
     gene            26347..27657
                     /gene="murA"
                     /locus_tag="PLD44_01325"
     CDS             26347..27657
                     /gene="murA"
                     /locus_tag="PLD44_01325"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOF98012.1"
                     /translation="MSSFQIEGGHRLSGSITPQGAKNEALQVICATLLTSESVIIRNI
                     PDILDVSHLLSLLKDLGVKISKIEKGTYSFKADSLNMDYLQSEDFYDKSSSLRGSVMI
                     LGPLLARFGKALVPKPGGDKIGRRRLDTHFSGIQKLGADFRYNETKKQYEVSAKKLKG
                     TYMLLDEASVTGTANILMASVLAEGKTTIYNAACEPYIQQLCRMLIAMGAKISGVGSN
                     LLTIEGVKQLNGCEHTILPDMIEVGSFIGMAAMTGSEITIKNVAYDELGIIPDSFRRM
                     GIQVLQKNDDIYIPAHESYTIETFIDGSIMTIADAPWPGLTPDLLSVFLVIAAKANGS
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLGNQLRLRASVMSSPDIRAGI
                     ALLIAALAADGTSIIHNIDQIDRGYEHIDTRLNALGANIERIQF"
     gene            27693..28205
                     /locus_tag="PLD44_01330"
     CDS             27693..28205
                     /locus_tag="PLD44_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOF98013.1"
                     /translation="MLNDKITEMLNNQINKEFFSWYLYLDISNYYANKNLDGFANWFS
                     IQAQEEYAHAQLFLKYLLNNGEKVKLLPIAAPESSFLDFKTPLSAAFEHELFITASIN
                     EIYAEAYAQKDFKTMQFLDWFVKEQGEEEKNVDDLCKRFDLFGTDPRGLYLLDNELAS
                     RVYTAPSLVI"
     assembly_gap    28516..28615
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            29034..30293
                     /locus_tag="PLD44_01335"
     CDS             29034..30293
                     /locus_tag="PLD44_01335"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HOF98014.1"
                     /translation="MKNILFAVVFVFLFVNSSLTAANPYRTKILKPNIKTLQIRISGT
                     DFGFPILKLNGTETLTVEFDEMSHNVRSFGYTVLHCNADWTVSNLNSNEYVDGFTRGN
                     IDDYAFSQNTTFQYIHYRFTIPNQDMRFKISGNYVVLIYEDNRVDDPVATACFSIVEP
                     KVEIDARVRANTDIELSGRYQQLDIEVNLANYVVQDPASEVKLVVRQNNRFDNEVANI
                     TPTFFNAGKLSFVNNKALIFEGGNEYRHFDISSFYAAGEGVDRIIFNRKYYEAFLKED
                     LMQKSRTYISQPDANGRFIINFQEAFQDVQTEADYVQVHFSLLSEQPFFDGQVFIGGD
                     YNYNLLDDTARMQYDFDAQRYTKTFLLKQGGYSYQYWFLLKGKISASAGKIEGSFWQT
                     KNEYTIYFYHRAWGERYDRLIGYSVID"
     gene            30596..32092
                     /locus_tag="PLD44_01340"
     CDS             30596..32092
                     /locus_tag="PLD44_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOF98015.1"
                     /translation="MNQEDTFPTTDSLKDFQQNFTKEPAIENLKNALNNEIFNIISDI
                     ADEAHQECYVIGGFVRDLLLHRPSKDIDILVVGNGIELAEKVVNQLKQLNKPCKFAIF
                     KNFGTAQIKLKEMDVEFVGARKESYRSDSRKPIVEDGTLQDDQNRRDFTINTLALCLN
                     KSRFGELVDPFNGLRDLQNKVIRTPLNPDITFSDDPLRMMRCIRFATQLNFFIETNTF
                     EAIGRNKKRIEIISKERITDELNKIILSPKPSIGFILLDKSGLLDLILPELSALKGVE
                     TKDGIGHKDIFYHTLMVLDNLSQTSDSLWLRWAALLHDIGKPRTKRFDKRQGWTFYNH
                     NIVGEKMIADIFRRMKLPTNEKMKYVQKMVSLHMRPIVLSEEEVTDSAVRRLLFDAGD
                     DIDDLMLLCEADITSKNPEKVKRYRENFQLVRKKLKEIEEKDRIRNFQPPIDGKEIME
                     TFHLPPCALVGEIKMKIKDAILDGIIPNEYEAARSYMMQIAAEMGLTN"
     gene            32106..33380
                     /locus_tag="PLD44_01345"
     CDS             32106..33380
                     /locus_tag="PLD44_01345"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOF98016.1"
                     /translation="MKRFFYFIPLLLLSCAIMAKNNGNPQKFKHSERSFAAFDTERLA
                     RLDTVFQDLVRKGEIPHAVTFVAHRGNIVHFKAFGWRDKEKNIPCQRDDIFRMASQTK
                     AITATGVLMLMEEGKILLDDPVKKYIPEFAHPQVLVSFNEKDSTYTTRPANKDITIRH
                     LLTHTSGISYGTDSNNPMMKIFAKKGIPPLNSLDPVTLKEVVKKLASCPLEHDPGERF
                     TYGMSVDVLGYLIEVVSKMPVDQYFKEKIFQPLGMNDTYFYVPKEKQNRMVTLYQKVQ
                     GKPLQPSSSVLYQTFPYSGAQTLFSTGAGLSGTIEDYAKFCQMILNKGEFNGKRILGR
                     KTVELMCRNQVGDLRGEIGFGLAFDDFRPQYLHLTMASEGSLRWGGMFGTDYLIDPKE
                     DLILLFYINWQTDNSVPDVKVLFHNLVYQALK"
     gene            33411..35525
                     /locus_tag="PLD44_01350"
     CDS             33411..35525
                     /locus_tag="PLD44_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOF98017.1"
                     /translation="MKAVIILSFSVVFMAACHSFSKIAYPHTQKENVTDRYFGTDVAD
                     PYRWLENDTSAATAAWVKAQNEVTFKYLEKIPFRNALKNRLTQLNDYPKLGTPFKKHG
                     KYYFFKNNGLQPQSILYQQSTLYSEPEILLDPNTFSSDGTVALSSVSFSNVGNYMAYS
                     VSKSGSDWNEIFVLNLDNRQTLSDHIQWTKFWNVAWKDNGFYYIAYDAPQKGKELSAK
                     NEFPKVYYHQIGQLQSKDLLIYQDKNHPLREFGPFVTEDEKYLFLIITETTTGNALLM
                     KDLTDPKAEFVSLVDGFENDYSVIDHINGKIYILTNWKAPNKRIMEVDPAHPSRENWR
                     EIIPEQKTVLESVEIIDGKFFCLYLKDAAHRLYAYDLSGKLLYEVKLPAHGSVVSLTG
                     NKDDSEAFFAFSSFTYPPTIYRLDAGKNVSEIFQKAQVKFKPEDFVTEQVFYTAKDGT
                     KIPMFLSYRKGIKKDGKNPLMLYGYGGFNISLTPSFSISRIPFLENGGIYAVANIRGG
                     GEYGESWHMAGTKMQKKNVFDDFIAAAEFLIAEGYTSSKKLAINGGSNGGLLVGACLT
                     QRPDLFAVAVPEVGVLDMLRYHLFTIGWSWASDYGTSEESREMFEYLKSYSPLHNVVK
                     GTKYPAVLIMTGDHDDRVVPAHSFKFAATLQEANAGNKPVLIRVDSNAGHGAGKPLGK
                     LIDAQTDMWSFVMYNLGMKPKW"
     gene            complement(35580..37271)
                     /locus_tag="PLD44_01355"
     CDS             complement(35580..37271)
                     /locus_tag="PLD44_01355"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOF98018.1"
                     /translation="MNDSYPYRNFGFRNNLDKVKRSRQIISVFVKFGLDYLFEASRVN
                     FLSKLRKRRKGYHKLSTAERLRLAFEELGPTFIKFGQILSTRPDFLPAEYIRELEKLQ
                     DQVLPMETPLVRQCIEKELKHTLEELFLDFDQSPVASASLSQVHKAVLQNGDTVALKI
                     QRPDIHKTIETDLSILESFAEILEKRLRNSSLYHPTAIVAELKKTIRKELDFMNEAHN
                     FEKFRKNFKNVDYIQVPKVYWQFTTEKLLTMEFIEGVKITEITQEKYQHRYEPKKVAD
                     RAAKALLKQILIDGFFHADPHPGNLFIQPPATIVMLDVGMTGHLDEESIITIARILRA
                     VNEKDSNHVIKGLQELGIVVKETDKRQLRHDLTELIEMYADRPFRTISLNRMNQQLLE
                     IMARHDMALPSNLVMMLRAMSIAESIGQQLEPDFDMFAIVEPFVKRIYLKLYSPKSFR
                     KRSMNVMDESIELIEHFPQNISDLLGKLKEGQFEIKLEHQNLENLQDEMKSSSNRISA
                     SLIIAALIVGSSLILQQRIGPLISDISILGVIGYLLAGIMGLGLLLTIFKTRRKK"
     gene            complement(37276..37593)
                     /locus_tag="PLD44_01360"
     CDS             complement(37276..37593)
                     /locus_tag="PLD44_01360"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOF98019.1"
                     /translation="MADVIKNAFLAGLGLVSLTREKAEEFAKDLIKRGELTETEKPKF
                     IKDLLEQSEKTKTEMEEKVEKAVDALLKKMNIPSRKEFEELKNKVEELTQLLNKKQEE
                     SEK"
     gene            complement(38268..38555)
                     /locus_tag="PLD44_01365"
     CDS             complement(38268..38555)
                     /locus_tag="PLD44_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98020.1"
                     /translation="MILKINKGRVEKYSTSGSYQGTITDNAIDADSNENLVVVVKSNG
                     RVEKYSTSGSYQGSITDNANRARISGDIIIVTKNNGRTEKYSFSGSYKGSI"
     gene            complement(39168..40574)
                     /locus_tag="PLD44_01370"
     CDS             complement(39168..40574)
                     /locus_tag="PLD44_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98021.1"
                     /translation="MRKIFLTFIVFAFANTVFSQNKQTTVIRPETNIEKTVREANNTV
                     GFINALGKWAQQVKADRLNKVKNSKYSVFVEQKLETWKKKGTFEKTADWQKRLNDSTT
                     IKKAEIYESALDEFVQVLEVLNNDNTSLNGFFYNSKGQYDSDKEVLVVNTFWGNIPIP
                     IPLDKAKNWQNIQNVNALKAHFFIQNDQLALLSLSTYDETYTWENPSQLAQIARKKML
                     ELEQKKKLELERMDSLELATYNQKLDSIFNNCNTKLLQNPYNLSKKLIIDYQKVTGKN
                     DRKYNYDKSVSSIKYSYERITDDIIQEYENEYSKNGNLFESKIEFDTFYKKGKGDYQS
                     EVERKKSEVERKKIFNYLTSNFQFIESMDFQKEKKESVASTMGKVFLGIATNTFTTNM
                     DYTSVNEIRKNILSVISDSQNKTYYPQVIDFVIATNKALNKEWNKNGQYFKNKEEFYN
                     AYLSEDYKNILKREKKQR"
     gene            complement(41155..42267)
                     /locus_tag="PLD44_01375"
     CDS             complement(41155..42267)
                     /locus_tag="PLD44_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98022.1"
                     /translation="MEWIILLVTFILCVPLVLMMVAGSCSDEVFEVIESVLNHLGGFG
                     DWCFKKLEAYRTSKAGKREDFAYADSFKDGVTYTSKEISNGVEREVSWTHSVPLSNGG
                     ISNLLFLGILPMLCVGYWLWFFSFGPDVPNWDIQDISFTIVWSLIVSAVLSGLGLFIG
                     LLRIINPVRVSVPDPNALNNKILVKSNPKVYKWVVRICVVSLAGTLYLFNYCGFGNVL
                     IKDFGDYDSGLAKGSSIRFYPRSAEDALADAFQYAVSESKNEDNKQRQEENIFFNGEQ
                     TEYYKLYYYGEDPSHEWQALNVKIIFGETEIKVITSEETITYILAGNPKYDDCTTYYV
                     YYSADPEKQIQEIIRCKDETQIIHESNCGEYEIFKK"
BASE COUNT        12822 a         8016 c         8046 g        13536 t
ORIGIN      
        1 gctacaactt ttgcaccgcg gtcgtgtcca tcttgcccca ttttggcaat cataatacgt
       61 ggacgacggc cttccttttt ggcaaattct tcagtaagct gcgaagcccg ctggaaattt
      121 gcatcgtttt ttgtttctga tgaatacacg tttgaaatag ttcttatgat tgctttataa
      181 cgacctgcca ctttttcgca ggcatcggaa atttcgccca gtgttgctct gacacgtgct
      241 gcttctactg caagctcaag caagttgccg ttaccggttc tggcacattc tgtaatggca
      301 tccaaagctt ttttcacttc ttcgttgttt ctggaagctc ggagctgttt cagtctttca
      361 atctgttgca tgcgtactgc cgtattgtcg atttcgagaa tttcgatggg ttcttctttt
      421 tccagcgtat atcggttaac tccaacaatt atttgtttcc cggaatcgat gcgtgcttgt
      481 gtacgggcag cagcttcttc aattttcatt ttcgggatac cggtttcaat ggctttggcc
      541 attcctccca gattttcgat ttcctgtatc aagtcccatg ctttttcggc caattctttg
      601 gtcaaggatt ctacataata agaacctgcc caggggtcaa tttgtctgca aatattggtt
      661 tcttcctgaa gataaatctg cgtgttgcgg gcaatacgtg ccgaaaagtc ggtaggcaag
      721 gcaatagctt catccaaagc gttggtatgc agcgattgtg tatgacccag cgctgctgcc
      781 atggcttcga tacaggttct tcctacattg ttgaaagggt cctgttctgt aagtgaccat
      841 cccgaagttt gggagtgcgt acgaagtgcc atagactttg gatttttagg atgaaactgt
      901 ttcacgattt tagcccagag aagtctggcc gcacgcattt tggcaatttc cataaaatga
      961 ttcattccga taccccagaa gaaagataga cgaggtgcaa aagcatcgat gtcgagccct
     1021 gcattgatac cggtacgcaa atattccaaa ccatcggcaa gtgtataggc cagttccaaa
     1081 tcggcagtag cacctgcttc ctgcatgtgg taaccggaaa tggagatgga attgaacttg
     1141 ggcatatttt gcgaagtata tttgaaaata tcggcaataa ttctcatgga aaattgtggc
     1201 ggataaatat aggtgtttcg caccataaac tccttcaaaa tatcgttctg gatggttccg
     1261 gaaagttctt cgagtttagc tccctgttcg agccctgcca caatatagaa agcaagaatg
     1321 ggcagtacgg cgccgttcat ggtcatggaa acggacattt tattcaaggg aattccgtca
     1381 aagagaattt tcatatcctc tacggaacaa atggatacgc ctgctttccc cacatcgccc
     1441 acaactcttt catggtcggc atcgtaaccg cgatgcgttg gcaagtcgaa tgctacggaa
     1501 agcccttttt gacctgcagc caaattacga cgataaaaag cattggactc ttcagcagta
     1561 gaaaaacctg catattggcg aatagtccac ggacgcgtta catacatgct gctgtaagga
     1621 cctctcaaaa aaggaggaat tccggctgca taatgtaaat gttccattcc tttccagtcg
     1681 tttttctcat aaaaaggctt aacgggaatt tgttcgggcg ttagccaatt ttcttcagtc
     1741 tgaagcatgg cagctccact tgaagtaatg ttgctgacat caatgttgtt aaaattcggt
     1801 ttcatatttt ttttgttttt tgaatcagac tttccgacga cgaaaattca atggtcagtc
     1861 gaaaatactg tatttcaatg tttacttttt tctaaaataa aatcattaat cgataataaa
     1921 aaatctaata ggacaagtta ttgtaatttt tcagttttgg acgttgataa tttcattttt
     1981 cacacgatag ggaataatat aaaaacggaa acatcccaca ccgcatgtga aacaatcaag
     2041 gttaccaaat ttttattgta tttgtaaagc aatccccaaa atgcaccgca aaccatggca
     2101 gacataatca gcatgaagtt aaacgaccaa atgtgtacca aagtataaat cagcgttgta
     2161 actatcaatg ccatccatga accaaatttt ggttccagca ttcgctgaac gtatcctctc
     2221 caaaaaattt cctctgccgg gccaatcaaa agaatgagca ataaactcaa caacaaagga
     2281 ttttctcctt ctttcatcat gtaaatctgc ccaacctgat cttttgcaaa atcgaacagc
     2341 aattttgaaa agaaatcgcc caagaaaaac accacatata aaacaacagc cgatcccaga
     2401 ccaatgagta catctttcag cgaaagcgaa aattgttttt tgaaatcttt tccgagaaaa
     2461 gcactcatca aagtcagcgt tatgccggca gccgtcatgg tcagccagaa gttgacatat
     2521 tctttggtcc agggagaaaa catgacaaac caaaaaatga tagcaatcag tatgggaata
     2581 ataattcttt tcataaccat gcagcaataa aatttgaaat agagaacaac aagctgaata
     2641 caagtaccaa ctgtgcagtt tgtgcatcca aatcaaaaat cttttcgggt ttttccactt
     2701 ctgcggtcat catttttttg atgtttttca cagccaaagg caacgaaagc aacatcagca
     2761 aagcaaccgg atgaatcatt ttgaaaataa tcatcaacac cacacccaca taagctccaa
     2821 tactcaaaac aacatactgt atttttgcac ctttgatacc caacagcatg gcttgtgttt
     2881 tgatgttggc ttttccgtcg tcgcgaatgt ctcttgtgtt gttggcatgt aaaatgttga
     2941 caaccaagaa ggcaacaggt atattcaaca ccactgcaaa ccacatgagt tgtccggtca
     3001 tgacgtaagc tgtacccaac ccgatcagcg gcccgtagat gataaatata gtcaaatcac
     3061 ctagcgcagc gaatttcaat ttgtaataga aataagttgc cattgcgcca atcgctccga
     3121 tccacaacaa atcgatgccc gttcggtaac aaagaaaaag tcccagcaca acacctaccg
     3181 ccagcaaaac aatgccaaaa tgcaaaatcg ttttgggttg aaaaacctga tcgaccaaca
     3241 ttcggcttga accaaacgtc tctttacggt ccacatggtg ttgatagtcg aaataatcgc
     3301 caatcagatt tccgctggct tggaaaatgg cggcacccaa aagtgccaac accccaaacc
     3361 accagttaac ctcgcttcca agctcatttt tcagaaaaaa gacatacgaa atggtcaaca
     3421 atgcaggcat ggttgaagca ggaaaagacc agggcctggt agccagcaat ccgtttttaa
     3481 ttttttcttt cattttgaag atgttggata gaaaaaattt tttgactgga gaatttattt
     3541 tttataaatg aaaatgatat acaaaaactt tacagaccca gttttttgtt gaaatccttc
     3601 aaggtttcca acacattgga ttttatgctg ataaagttct caatcccttc ctttttcaaa
     3661 tcttccatac aagcgggagc gcctgccaca acaaaaatcg ttttgtcctt caacaatttg
     3721 taagcttcgg gggctaatgt ggcatattct tcgtcgctgg agcaaagcac aacaatttcg
     3781 gcttttgcat ccaacgctgc ttttacgcct tcttccacag aagagaatcc gagattgtcg
     3841 ataatttcgt atccggcaca tccaaagaaa ttgcccgaaa attgagctct tgccgagcgc
     3901 atggctaaat ttccaacggt caacataaac acttttggac gcttggacgt tttttcgacc
     3961 gctaagcgta agttttcaaa ttccacagca ccacgcaacg gaagaagctt ttctgttcct
     4021 tcttcatgcg aacagcaacc acactgttgc aattccttgt cgattttttc ggcagcagtt
     4081 tccaataaat ttggatattg attggtgcct accaaaattt ctttccggtt cgagatagct
     4141 tttaaacgtt cattggctgt agccttgatg gtctgttgaa cctttccgtt ttgaacggca
     4201 gcaaggaatc cgccttcgtt ttccacattg aggaaaagtt tccaagcctg attggctatt
     4261 tcggcagtta aagtttcgat ataataggaa ccggctgccg gatcaacaac ttcactgaaa
     4321 tgagcttctt ctttgaggag taattgctga ttgcgggcaa ttctttccga aaattcgtcg
     4381 gaaggtttga aagcagtgtc ataaccgagc accgacaaag aatcaacacc tccaatagtt
     4441 gcactcatgg cttcggtttg tgtacgaagc atgttgacat aagcatcgta aatggtttga
     4501 ttgaaaatgg tcgtttcggc gtggatacgc atttttgctg cacatttgca aattccattt
     4561 actgtattgg gacagtcggg acgaggacag ggaggttgat aagcatcaac aattaatgcc
     4621 cacaacatgc gtgcagcacg gaattttgca atttccataa aatagttgct gccgatacca
     4681 aaattgaatt ttattttttt ggcggcaacc gctttatcca ctcctgcgtc tatcaacatg
     4741 gaaaggtatt cgtttcccca tgccaaagcg taaccgagtt cctgggtaat ggtagaacct
     4801 gcgttggaga gagtggtggc attcacgcca attacacgat aaaaaggcaa tctgcttatt
     4861 gcctgaacaa cagctttagc cttttcggca atctcttctt tcgtcaattt tttgcctgcc
     4921 agcaacatgc gattaatcgg gtcgaaattg atagaaccct gcaatttctt gacctcataa
     4981 ttttgagcaa tgaaatactc ggagagaata tcggcaagtt cggcagcttt gctgatgcaa
     5041 atcgtgaaat tcaactccac acagtcagct gcaatatcct tgagcaaagt ggcaacaaat
     5101 tctttagata gatctgcttt tttcagctga aatccaatgg agttcacccc tcgatttaga
     5161 atatacaggg ctttagcatt ggcttcctgt gcgttttcaa cttttatgtc ctgccgtaca
     5221 aaccattcat tatcgctttt tgtccccctc acataaggga aagctccggg caacaattcg
     5281 gtgattttca gatcatccac atcttcctcg cggtaaaagg gtctcacatt gaaaccttca
     5341 ttggttctcc agatcatttt tttctcaaaa tcggctccct tgaggtctgc aatgatttta
     5401 tccagccatt cctgcgtgga gacaggagga aaatcagcta ataaatttaa ttttttttct
     5461 gccataatat caaattttta tcgaaatttc tttgagctgc aaaattacta ctttaaatcg
     5521 attctttaag attttttggg attaaataaa gaaaaaaagt ggtaattagt tttgaatcta
     5581 agctgaattt atataaacaa tgacgagaaa tttttcctaa gaaaaaaatt aaatacttgg
     5641 catacaatta tctgacaaaa aatgaactaa aaaatatttc aactgccttt attcaattca
     5701 atttttttaa ttacatttgt ttttcaatgc aaacgtttgc gtcaatttaa aaaatatgaa
     5761 tcatgcaaaa actaattatt tttatttttt cggttttatt tgcctttcct ttatacgcca
     5821 aacgcgtaat gacagggatt gaagtactca gagcagataa tttcaaattg ttggaaggca
     5881 aaagagtagg tttgattacc aatccgacag gtgttgatgg aaatttaaaa tctaccatcg
     5941 atattttata tgaagcacgc aacgtaaaac tggtagcctt gtacgggcct gaacacggcg
     6001 taagaggtga tgtttatgca ggcgataaag ttgtgaatgc cacagatgag aaaacaggtt
     6061 tacctgtgta ttcgctttat ggaactacac gcaaaccaac ttctgaaatg ttaaaaggaa
     6121 ttgatgtgct ggtttatgac attcaagatg tgggttgtcg ttcctatacc tatatcagca
     6181 ccatgggttt agctatggaa gctgctgccg aaaacgatat agaatttatt gtgctcgatc
     6241 gtcccaatcc actcggtgga gaaaaaattg aaggcaacct gacggaagaa ccttttattt
     6301 cttttgtcag tcagtttaaa attccttata tttacggtca aacttgtgga gaactggccc
     6361 taatgttaaa tgaagaaaac gcactgaaaa agaaatgcaa actgacggtt gtcaaaatga
     6421 agaactggaa aagaagtatg aaatgggaag acaccggatt ggaatgggtc attgcttcac
     6481 ctcacattcc gcataaacat tcagcttact actatccggt taccggaatt ttgggtgagc
     6541 tgggttacgt atcggtaggc gttggctaca cccttccatt tcaagttgtt gcagcgccct
     6601 ggataaaagc acaggaattt gccgatgcca tgaatcgttt acatttgccg ggcttcattt
     6661 ttcgacccat ctttttcact ccattttatg gaactttaaa aggagaaaat tgtcagggag
     6721 tgcagattca cattgtcgat ttcaaaaaag cccatctgtc tttggttcaa ttttatatta
     6781 tgcaggagtt ggcacgtttg tatccggata agaaagtatt cgacaatgcc aacaaagaaa
     6841 gattttccat gtttgataag gtaagcggaa ccgatcaaat gagaaaactg tttcaagtta
     6901 acaattcggt atccgacatt cagcactatt gggacaaaga tattcagtcc tattatcaac
     6961 tttcaaaaaa atattatttg tatcactgat caaatgaaat gataaactcc tttttatccg
     7021 aaattaaaag ttttatcccc gaaaaacgaa tttacaccga cgaattacgt ttactcgcat
     7081 ggggcacgga tgccggcttc tatcgtttga taccgaaagc agttgttcaa tctgccagcg
     7141 aaatggaagt cagtttgctt ttacgcgctg caaccaaaca tcatcttccc gtaacttttc
     7201 gtgctgccgg taccagcctt tcgggacagg caatttccga ttcggtgctg attcttgccg
     7261 gtagcaactg ggaaagatac gaacttgctc ccgatgcttc tttcatccgc ctgcaaccgg
     7321 gaattgtggg agaacgcgtt aatcagattt taaaaccttt tagaaaaaaa tttccacccg
     7381 accctgcttc aattaaatcg gccatggtgg gaggaataat cgttaacaat gcttcgggaa
     7441 tgagttgcgg aatacgcgaa aacagttata gaatgttgat ttcgacaaga atagttcttc
     7501 ccgatggcag tgtgttggat acgggcgacg aagaaagtaa agcggttttt gctaaaactc
     7561 atcccgaatt tcttcgcaaa atagaagatt tgcgagacga aatacgcaac gacgtaacat
     7621 taaaacaact gattgaaaag aaataccgga taaagaatgt taccggttta tccatcaatt
     7681 cttttatcga ttttgacgac ccattcgata ttattacaca tttaatggtc ggttcggaag
     7741 gaacactcgc ttttctttcg gaagcagtaa tgaaaacaac taccgactat ccgtacaagg
     7801 caagtgctat ggcatatttc gagaacatgc atttagcttg tgaaaccgta aaaaatttaa
     7861 aatcttttcc tgtcagtgct gtcgaatttt tcgaccgtaa agccattcgt tcagtagaag
     7921 acaaatttga aacgatatct gagctaaaaa ctttgccgga taatagtgca gctttactca
     7981 taaaaattga agcatcagat ggaaatgaat tgaatgaaag aattcgacaa gtggaaaaag
     8041 tcctgaatga tgctcatacg cttctacctg tgagctttac cgacaaagaa tccgaataca
     8101 aaaaatattg ggacatgcgt tcaggaattt ttccttcggt gggtagcacc cggccagtag
     8161 gaacaacttg cctgatagaa gatatagctt tctgcataga agatttagcg gaagcaacag
     8221 acgatttgca cgaaattttt ataaaaaacc actatgaaga tgccgtcatt tacggacatg
     8281 cactcgatgg aaatcttcat ttcattatca gtcagcgttt tgatacggaa gaatatattc
     8341 atcagtatga aaaaatgatg aatgaagtaa ttgaaatggt ggtaaaaaaa tatcacggtt
     8401 cgctcaaagc cgaacacgga acgggcagaa atatggctcc atttgtaaaa aaggaatggg
     8461 gagaaaaggc tttcgacatt atgctgcaag tgaaacaact tttcgatccg gaaggaatcc
     8521 tgaacccggg agtaatattc aatgacaatc cgcgttgtca tttggaaaat attaaaccca
     8581 tgccggctgt gcatccgctt atcgataaat gtatcgaatg cggcttttgc gaagtaaatt
     8641 gtgtaagtgc cggtttcacc atgtcttcgc ggcaacgaat tgttgtgcaa cgcgaaatta
     8701 aaagattgaa aacttctgat gaaaattccc gacgcttgaa ggaattggag aaaagttatc
     8761 gctttatggg agaacaaagt tgtgccggcg atgggctgtg tgcaacatct tgtccggttt
     8821 cgatcaatgt tggcgactat atccatgtgc ttcgcaatga gaacatgaaa aacaaaacaa
     8881 tggctcaatt tttgggacgc tggactgcca aacatttcaa ttgggtggct tcaggtttga
     8941 aatcggtact ctatattgct tccgctgcac aacacatatt gggtaacaaa gcaatgggtg
     9001 caattactcg tggaatacac aaatggagtg gcggtgtttt ccctttgtgg acaccggcca
     9061 tgcccaaacg aagaaataaa atagaagtat caacttcaac ctcgactgac tcatcattga
     9121 aagtagttta ctttccaagt tgtttgaatc agatgatggg cgtaagtaaa aatgacccag
     9181 agctgacgcc acttgtagaa aaaaccgtat ctttgctcca taaagcaggc taccaagttg
     9241 tttttccaaa aaacatggaa aaaatgtgct gtggaagcat ttgggaaagt aagggattgc
     9301 tttcggtgga cgaacagaaa tcgtcagaat tggaaaaagc actacgtgaa gcttccgaaa
     9361 acggaaaaat tccgattctt tgtgatcaaa gtccttgtct ttatcacatg cgcaagtcga
     9421 tgaaacaact ctcactatat gaaccggttg aatttataca cgattttcta ctggataaat
     9481 tggattttta ccctatcgat gagcccatta ctatccatgc cacttgctcc accatcaaaa
     9541 tgggattgca gccaaagctt gtagaaatag ccaaactttg cagtactcag gttttggtac
     9601 ctgaagaagt tggatgttgc ggctttgccg gcgacaaagg atttaccatg ccggaactga
     9661 atgctcatgc gttgagaaaa cttcgcccac aaatagaaaa agcccatgtt aaagtgggtt
     9721 acagcaacag ccgtacctgc gaaattggtt taaataccaa caccggaatt ccataccagt
     9781 cgatagtata tttggtggat aaatgcacaa aaaataaaat aaacagatct taaaaaattt
     9841 cacgaataaa atcatgagaa aatttcgttt gtcagtagtt ttattattct ttgcattctc
     9901 gtttttgcag gctcagccga agcgggaaat gagggcagtg tggatagcaa cagttgccaa
     9961 catcgattgg cccagctcca atcggttgtt acccacccag cagcgaatcg aaattgtgaa
    10021 catgctcgat cggctgactg aaaacaacat taacgccata gttttgcaaa ttcgtccgat
    10081 ggctgacgct ttttatcctt ctaatctgga gccctggtcc cattggctta ctggccgaca
    10141 gggcgtgcgg ccttcacctt tttacgatcc gcttcagttt attatagaag aagctcacaa
    10201 acgctgcatg gaagttcatg tctggctaaa tccctatcgg gtaacaacaa ccggtgattc
    10261 catgatgcta tcgaaagagc atttgtttta caaacaaccg gagctttttg ttcgttatgg
    10321 cggaaaatat tatttcaatc ccggactgga tgaaacacgg aattttttaa acaaagtggt
    10381 ggaagatatt gtagaacatt atgatgtaga tggggtacat tttgatgatt atttttatcc
    10441 ttatccggtg aaaggtgaag attttccgga tgatgaaact ttcatgaaat ttccaagagg
    10501 ttttgctccc aatcagaaag ccgactggag acgtaacaat gtaaatatgg ctattgccga
    10561 agtacaacaa accattaaaa acctcaaacc ttgggttgaa tttggcgtaa gtccttttgg
    10621 cgtatggaga aacgacaatg tcgatccacg tggctctgca actcgcgctg gcattcaaaa
    10681 ttacgacgat ttgtatgccg atatattgaa atggctggcc gaaggaacga tagattatgt
    10741 tacaccgcaa ttgtattggg aaatcggtaa aaaagttgct aactacgaag ttttgttgaa
    10801 atggtggata gaaaattctt acggaaaaaa tctgtatatc ggattaattc cgtcctcttt
    10861 gggcgataaa aaaaatccgg catggacaac aggaaatgaa attattcgac aaatcaaaat
    10921 gaatagaaat tcctcacaag tggacggagc cgttttcttc agtgcaaaaa cctttctgca
    10981 aaacagacag gggttaaatg acagcttgca atcgaactat tatcgttatc cggcactatg
    11041 tccaataaac agaaatattt taggcgaacc ttctgcgcag ccgcaaaatt tgcgggtttt
    11101 gagagatggc aaggatgctt ttctgttgtg ggatgaagtg aatgaagaag gaggctgcca
    11161 aactgcctat tatgtggtgt atgcttttaa gggaaaagaa gttggcgact tgaatgatcc
    11221 ggtcaacatt atagctcgaa caaccgaaaa ttgcatcgat ttgcgaatcc taccccacaa
    11281 atttaaggga aattatacat ttgtggttac tgctgtcaat cgtttcaggc aagaaagtat
    11341 tccgacgcaa ggaatcacac gtaagttgta atttctgatt tgcgaaaaaa taattttaaa
    11401 gaagaggact gaaaagacgc gaaacggaat ccatcagttt ttttgttttt tttcggttgt
    11461 tccactgttc cagcgtaatt tcctcacagt aatgtatatc ttccaagaac agctctttaa
    11521 gtgtaagggc tgttttttct tcatatacaa aagcattcgc ttcgaaattt tggtaaaaac
    11581 ttcgataatc aatgttacaa gagccaacaa tggaaacaaa atcatccatc accacagttt
    11641 ttgcatgtaa aaaacctttt cgataataaa aaacacgaac tccggcctgc agcacttttc
    11701 ccagataact ggaagtactg gcaatgacaa ttggcgaatc ggattgtgtc ggaatcatta
    11761 tcctcacatc tatgccactc aaggcagcca actgcaaaca gccttctatc agttccgtcg
    11821 gcatgtaata gggtgactga atataaatat atttttttgc cgacataatg gctttggcta
    11881 tgccttgcat aatggcttcc cattcggtgt cggggccact cgaaacaatt tgtaccagat
    11941 tgttgtcaaa ctgcgctggt accggataat actttttgtc ggtaatcagt tttttttcca
    12001 caaaatacca gtcgctcaga aaaagatgct gtaatccatg aacagcagaa ccttgaattt
    12061 tgagaaaagt atcgcgccaa taacccagac ggttgccata cacgtatcgg tcggcaatat
    12121 tcattcctcc ggtaaaagct acttttccgt caacaacaag taatttgcga tgattgcggt
    12181 aattgatccg gctgcgcgaa attcggaatc gaagcgggaa aaagggacga acgtaaattc
    12241 ctgcttttgt caaagttttt aaaaattttc gagaaagatt aaaacttcca ataaaatcgt
    12301 aaataattct gattctgact ccttcgtgcg atttttttat caataaatct ttcagccgat
    12361 ttgtaatgct gtcgtcgctg attatgtaaa actccaaatg aatatgctcc ctggcctgtt
    12421 ccacagcttg aaaaatggat tcaaaagctt ctttcccgtc agaaaaaact tctattttat
    12481 tgtaggtata acctactgct tcgctattac ggtacaacag tttgattaaa ttcatttgat
    12541 gggcatccag caaatccgaa ttgattttac tgaaatcgaa tgaagaaatg tcgcggtgat
    12601 cgagactgtg aatactttta cgtgaaataa ttttctgttt gcgcaaatcc tgtccgaaaa
    12661 tcaggtaaag aatcaatcca ataaccggca gaaaaagaat aatcagcatc cacgacaagg
    12721 atttcgacgg attcctgttt tcgagcaaaa gcacagaaat tgttgtcagt atggtataga
    12781 catacaaggc tataaaaaaa atcgaaataa tttccgacat ttacttaatt gtttttttcg
    12841 ggttataaaa atgaagccat tcgtgtccgt caaaattttt tctgctcgcc ggttgacaag
    12901 tcataactgg tgcggcaatt ccgttgccaa tttcgattgg agcaacttct acattggctt
    12961 catcccacaa attttcggct ataaaactat tcagcaattg cgctccacct tccaccagaa
    13021 cagaatgaat attccgatca aaaatttcct ttaaaattcc gcgtaatcct tcttttttaa
    13081 aatcaatttg aatatattcc acattgttaa gttttgcagg ttgcctttcg gtaaaaacca
    13141 acgtaggaac caaaccatcg agcaaatgat aattttccgg aatccttccc tgtctgtcga
    13201 gcgtaattct gatgggtgtt tttcctgtcc aattccttac ggtcaaactt gggttgtcga
    13261 ggagcacagt attggtgctc accataatag actgattttc agaacgcatt ttgtgggtca
    13321 gttgtttggt gatttcgttt gaaattatca aaggcggttc gctgacatca tttctaatgc
    13381 gatcaataaa tccatcaatg gtttgtgccc attttaaaac aatgaagggt cgtttttgtt
    13441 cctgaaaggt aaaaaaacgt ttgttcagtt ctctacattt ttcttcaagt actcccacat
    13501 acgtttcaat gccggcatct ttcagtattt ggattccttt tcccgaaact ttgggattgg
    13561 ggtcaatcgt gccgataaca acgcggggaa tacctttttc aacaattaaa cttgcacatg
    13621 gaggcgtatt gccatagtgc gaacaaggtt ccaaattaac gtacagcgta gcatctttca
    13681 gtaattcggg ttgttttacg gaaaaaatag cattgcgctc ggcgtgtgct tgcccaaaat
    13741 gttggtgaaa tccctctccg ataatggtat cgttataaac cactaccgca ccaacaagcg
    13801 gatttggagc agtgtatcca tccccaagcg aagccagttg caggcatcgt tgcatatatt
    13861 tgatttcaat atccatgtcg gttctttata aaatcagttc taaacaaaag aaattgtaac
    13921 aaaaaaagct aacgagaaat tttggatatt ctttatcttt gtatcatgga aaatattctt
    13981 caatacatac attccgagct gaaaggaatc tatcctgaaa gtgaaattcg atttatcggc
    14041 tatttgttgc tcgaaaaatt gaccggtttt tcacgaacag aaattttgct aaacaaaaat
    14101 acacaaattt cttgtgaagc agccaaacaa ttggatggtt ttttaaaaaa actaaaaaat
    14161 catgagccgg tacagtatgt tttaggagct accgaattct gcggactgag tttcaacgtg
    14221 aatccgtcgg tactgattcc acgacccgaa acagaagaac tggtggaatg gatatattct
    14281 gaaaacgacc gaaacaaacc gttgaaaatg ctggatattg gcaccggaag cggatgtata
    14341 gccatcagtc ttaaaaaaaa ctttccattg gccgaaatag atgcttttga tgtttcgcca
    14401 gaagctttga aagtggcaaa agaaaatgcc aaactcaatc gggtaaatgt caatttcaga
    14461 gaaatagata tttttaatcc tcctgcattc agtaaaaaat gggatttgat tgtcagtaat
    14521 ccgccttatg tgcccgaaaa cgaaaaaagt gaaatgcaac gcaatgttac cgattatgag
    14581 ccttccttgg cattgtttgt gcctgacaat catcctctga aattttatga aacaattacc
    14641 aactttgctc gattaaactt ggcaaaaggc ggaaaactat attttgaaat tcactatcga
    14701 agtggaaaga acatgctgga tttgctttcc caaaacggat tcagagaagt tcaattgaaa
    14761 aaggaccttt ccggaaaaga ccgaatgata agtggaattt tttagaattt gtttgaaatt
    14821 tccgatgatt tttctgctga caaaagccga aaaaaacata cagcaaattt ttaataaacc
    14881 aatccgttgg aaagaacccg gttcaatcat gaaacaactc tactttaaag ggtctttaaa
    14941 tgatatttga atgcttttta aatgttgttt tttgtggaaa gcatatttat tttttgtcga
    15001 aatgtaattt tttctcaaca aattcaataa tataggagat tgtttcttcg attcccaatg
    15061 cggacgaatt gatgcacaaa tgataggaag cagccgcacc ccattgttta tcggtataat
    15121 aattgtaata atcggctctt ttcttatcca ttttttttat gtagtccatc gctttttctt
    15181 ctgtcatttt ctgattgcgg catacgcggg caattctgtc aggcaaatcg gcagtaataa
    15241 atatatttac tgctcttgga tgatccttta gcacataatc cgcacaccga cctacaaaaa
    15301 cgcatgattg cttttcggcc aaactgcgaa tcacatcgct ttgaatctta aacagcattt
    15361 cactgctcgg ataattatca ttaaaataat attcatcaat cataatggaa tttcggagtc
    15421 caaacattcc tctgaaaaat ccctgacctt ttttttcgtc cagctgttcc agaaattctt
    15481 ttgccagccc actttcctgc gaggcgatct gaatcagttc cttgtcataa aacgatattc
    15541 ccaattcgcg ggcaattcct tgcccgatca aatgaccgcc gctacccaat tggcgtccaa
    15601 tgtttataac aaatctttga ttcataaata tagttaataa cttccactct ttttcgacaa
    15661 atttttactt catttattcg gtttttaaag caaaacaaaa tattttttcc ccttttgctg
    15721 taaaaaaata ttttattggt cttcattctt taaagaaaca aggatgagga tgaaaaattt
    15781 taacataaaa caaaagtaca aaatttggat taaaattcaa atgcgatcga aataaaaaac
    15841 tcctgtcaga tgaaactttg ttcttaattt tgcttgttgc ctattataaa tttcttcaag
    15901 aaaaatgtcg agactgacaa agaaaatata caataggatg aattctggcg tttgcttttt
    15961 ttacttttta tcaaaatgtt tatcggtcaa tagtttattg tattgatatg attttttatt
    16021 ttatcaacac ttctaatgga gaacgataac tttgtgcaaa aagatttact gcatttatcc
    16081 agtcgttgtc cgttggaaaa ccccatttgt tccaacctcc gccaaaaaaa tagacaaaag
    16141 tatttcccgg ttgatattcg gaaataagca gagcgtgatt gtttttaatt attgctttcg
    16201 taattggagc cggtacaaaa attccaacgt aatttctgcc tgaaggaact ttggcatccg
    16261 aaacggcatc ttcacaataa gccgctgttc cttcttcttg gttcatagag agtttaccgg
    16321 tattttcatg gagaaatatt ccggctgcca atggtaagga ctgttgatag ccgttaaagg
    16381 taacttcggc tttatttagc aatgttcccg cactgacggt aatagttaat tgttctttgt
    16441 agaacttgtc tccaaccttt atcgaatcgt aaaataaaga gaatttcgat tgaagcggcc
    16501 caacataatg aactttgtaa ctgttgaaat aattcccaat ccaaagagaa tcgttccaaa
    16561 aaggagcaat gccgccagcc cccaatgtgt gcccaacttt gtagcagtca agtcctttcc
    16621 catgatcaat gtggtaggat tgtccttttt gaagttcgtt aaagtaaaat gtatcaacaa
    16681 tgagttcttt ggtacgtttt agccataaat ctactccgtt gcttggattt tctccggcca
    16741 aagccggccc gtacattcga taggctgcca agtcgttttc ccatgcaaaa tcatcttttc
    16801 tctcaggtac aaagcgagca aaagtctttg gttctaccgg cgaaggattt ccttttttca
    16861 agtgataggc aacttttttg tttgcaggga cagcagcctg aaaaataaaa gtttcaagat
    16921 ttccattctc atttttaacc aattggtaac caatttcctg attgtgattg ttctttaaaa
    16981 tccatttgtc gctttcttca aacagttgca aaaattcttt gtcatttatt tgaattattt
    17041 cgtcgtttcg atcaaatgaa cccgtattgg aaatttcaat tgttttaatt gaagacgaac
    17101 aaccggccat gagtacagta gccagataga ggaatatttt tttcatgatc taaaagaatt
    17161 ggagtcaatt atttttcctg tacggaaatt taattgactc gttgaaaaat ctttgtttaa
    17221 ttaagaaggt tgttttccca aataagccaa aattccgcca tctacataca gaatatgccc
    17281 atttacaaaa tcggaagcgc tggaagccag aaaaacagca ggtccctgca aatcttcagg
    17341 agttccccaa cgtccggcag gtgtgcgggt aacaataaat gtattgaaag gatggccttc
    17401 ttcgcgcaaa ggagcagttt gaggggttga gatataaccc ggcccgattc cgtttacctg
    17461 gatattgaaa gctccccatt cacaagccaa attttttgta agcattttca atccaccttt
    17521 ggcagcagca taagccgaaa cggtttcacg acccaattcg ctcatcatag aacagatatt
    17581 gataattttt ccgccgcctt tggctatcat acccggagca acggcttttg aaacaataaa
    17641 aggaccgttc aaatcaatat ccaccacttg tttaaaatct ttggcgctca tttccaatgc
    17701 tggaattcgc ttaatgatac cggcattgtt taccagaata tctatggttc cgacttcttt
    17761 ggtgattttg gcaatggctt ctgttactgc ttcttcgtcc gtaacatcaa aaacgtaacc
    17821 atgtgcatca atgccatcgg ctttgtaact ggccaatccc atgtcaactt tttcctgatt
    17881 gatatcgttg aaaactatta ctgctccggc agcatgaaaa gcttttgcaa tggcatatcc
    17941 aatgccgtag gatgctcctg taatcagagc aatttttcct tttaaatcga attgattcat
    18001 gtcttttaat tgttattttg attcgttgat aatttattga ttttcatgta aataattaaa
    18061 tattaatcga atttaaatat tatttcagat cggttttttt gtaaaaatcc tgatcatcat
    18121 aatccaaatt ttcacctgcc attccccaaa taaaagtata attgcttgta gcagcagccg
    18181 aatggatgga ccattgtgga gaaataacag cctgatcgtt tttcatccag atatggcggg
    18241 tttcctcggg ttcgcccata aaatgacaaa cagcttgatc atcaggcaac tcaaaataaa
    18301 aataggcttc cattctacga ctgtgagtat gggcaggcat ggtattccaa acacttcccg
    18361 gctcaagttc ggtcattccc atttgcagtt ggcaggtttc cagaacctga gaaaccatca
    18421 ttctgttgat aaaccgatga ttggagcctt ctaaactccc catttccaaa attaccgcat
    18481 tctcttttgt gatttttctg gtggggcagg ctttatgagc tgtagctgaa ttaaagtaaa
    18541 attttgcggg attggaacca tcaacgcttt caaaagtaac ttttttgttc cctctgccaa
    18601 tgtacaaagc ttccttataa tccaattcat aatttgtccc gtcaacgttg atacatcctt
    18661 taccaccaac attgaaaatg ccaatttctc tccgttcgag gaaaaaatcg gattttaaag
    18721 gatcaatcga ttccaatgta agtacttcgg caacaggcat ggcaccgccc accaacatcc
    18781 tgtcgaacag ggagtaaacc atattgacct cgtttatggt gaaaattctt tcaaccaaaa
    18841 attctttcct tagtcgtgag gtgtcgtaat tttttgcatc ttgtggatga gaagcatacc
    18901 gaatttcata attagttttc ataattaatt tttttgaatg ttatttgtaa tttttatgtg
    18961 tgcatgcaca taaaacattg tatgatatac gttttaattt ttgattttca tttttagttc
    19021 aacgaaattt tgttgattga gaaaatttca ttgtagtttc ccgataaacc aacttttagt
    19081 ttaactctca gtaaattaaa ctattatgac taaaaaataa aaagtaaaat aattttcgtt
    19141 tgaataaaca aagttacaaa aattttcgag accagagaat ttcaagttcc atgaatttaa
    19201 tgctgtttat tttacctgcc acaaccagcc taaattaacg gaaaaattta tattgttgga
    19261 actgtcaaaa aaatctgttt tattgttgga aaaaatatcg ctcaagctgt aattggctcg
    19321 agcatcaagc tgcaaaacgt tttttctgat tctgaacata aatcccaatc ctccacaaat
    19381 gccgtaatcg aaaggatttt caacagattt gacatgttgg actttttcag aagaaacggt
    19441 ttcgtttttt aaaacatttt ccgtaagtaa ataactgatt ttaggcccaa tattgaaaaa
    19501 aaatcttgat ttattgccaa aataaatatg cgtcataaat ggaagttcaa tataattcag
    19561 ccggcgactg taaattgaat cggtttcttt ccatccacgt tgaaaaaagt tcaattcaac
    19621 ctgtaaacct acattttttt cggtgatatg ccggaatatc aatcctccat tataaccaag
    19681 cagcatgccc tgattgaccg atggcgaaaa attgatcatg gaagcagata ttccctgagt
    19741 ggttccaaaa taaatttccg gtttttcaga gctgggttgt gcgaataaat tggcagaaaa
    19801 taaaaaaata caaaggaaaa tggatatttt gcgcatgaca ttatttggct tttcgttctc
    19861 ccatataaag tttgtcgttg atataaccgg aaaattccga ttgttttttg aaatgaaaat
    19921 ctgattgtat tccttcatca aaagttattt ccttcaactt tttagtaaga ttattttcgt
    19981 aatttttcaa aatgggaata aaacagttca acaaatcgta tccgagcaaa tcataacgtg
    20041 gtctttcact ttctacttcc cattcaaaga actgatgaaa tgttttttgg tattcttcaa
    20101 gagcggaagc atcgatttca ggtttaaaag gagacacata aattctgtca aacctttcta
    20161 tatattgatt ttcccagcca tattcctgat aaagcgtcat atcgaactca tcaatataaa
    20221 catagaattc tttcaaaaat ggggtaatta ggcttgaatt atctgtattg aaaaagagaa
    20281 gatttttttg atttcttttt aaaacttcag gaatactttg atttttgtta gaagaagaaa
    20341 aattaaattc gttaaactta tgacgttctt tcaacagcct gtttttcaat ctttccataa
    20401 acagattccc ttcatcaaga atgctgatgc ccggtagatt cacaaaaaca atattagcat
    20461 tgcgataagt tttctgaata attttatcta caagaaaatc tatctctatt tgagtatcgt
    20521 tgggattgaa ttgaaaaaga tacggattaa tgcctatttc atacactttt gaagaaaaag
    20581 gaataagaac gtttattttg ttttcttttg caaaatcact tatcaacgaa atttgattgc
    20641 tgtaagcagg tccaacaatc aaatcaacac ttttcatttc tggttttgct aaaatttctt
    20701 tcaatttttc gtccgatttt tcggtatcga aagtgaaaat ttccaatgaa taattattct
    20761 ctttggcctt ttttattgcc agtaagctgc cggcataaaa atctctaaag cgtccgtttg
    20821 cattttgctg agaaggagtg tcaagattaa aaggcaacaa aaaagctatt ttgatattat
    20881 tttcagtaga aatggccgta gcaatttcag gattttcaat atcctgcaat gcagagtcca
    20941 tttcattgtt attcagattt gttgatggta tcgaatcttt tttcaaaata gaagtcggaa
    21001 tcgttagttc cgtgccgatt ttcagtacgg gaaaggcatc cggatttagc ttcaaaatat
    21061 catcaattgt aacattatac gttctgctga tagaaaataa ggtttcattg ggttttacca
    21121 aatgtttgat agactgaggt ttatcttttg atggaagctg cttttcgttc aattcctttt
    21181 ttccacttgg agtaatcact tcaactaagt ctccttttat ttctgtctca ttttttgttg
    21241 tttgggtttt atatggaata aaaaggacgt ctccttccct aagaatattt ttagattgtg
    21301 gattatattg gattatttcc tcttcactga cattgtattt tttactgata ccgaaaagtg
    21361 tttgattttt ttcaacctta tgctgtaaga ataaagttgt tgaaggaagg gagttgtccg
    21421 tttgctgatg aagatttttt tgattttctt caaaagattt ttcttgcagt aatgaaagct
    21481 catttttcaa tctctggctt gcatcctcat tttcctgtat atttttttgt attggaatca
    21541 aaattttttg ccctgatttt agtccttttt gtatttcagg attaactttg ctaatttctt
    21601 caggggcaat gccgaatttg cgacttatgg catacaaacc atctcccgat tgcaccgtgt
    21661 acacataata ttcaatacca ttcacttttt taatagggta ggagttggtt tgttccattg
    21721 aaaaacccgt aagcgacaat atgattacag tcagcaaaca gaagatgttt tttttcattt
    21781 gatggatatt tggagaaaaa tacttgtttt atgacaattg ataaagtttc tatgaatccg
    21841 gctgctaaac cttgcaaaaa tataaaaaat taatgaaaag aggaaagcag gcaataaatc
    21901 aaaggttttt ttgtttttga aaagaaaaat gtacctttgc taaactgtaa atctgacgtt
    21961 atcattcagg ttatatgaaa aaacttacga tttccttttg tttctatctt attgctttat
    22021 ccgtttcggc tcaacttgtt gcaacagata aatatgctgt ttatcagaat tttacaggtg
    22081 tcgattatct cttcgttttt aatggaattt tacccggaac cgaaatacgc tttacaggaa
    22141 gtggtgataa tgtgaagtgg tacagatttt ctgacacgac aacaccatac agtactttac
    22201 cctatatatt tcctgaagat gcaagcggat atattttgga tgtcgatggt catcaaactt
    22261 ctgtctgggt gattgattac caaaattatt tgcccgtttt tcatacgctc gcttctgacg
    22321 aatcgagcga taatcggtgc aacgagttaa ctttattgct cgatgctaat gtgcctgaac
    22381 tgtattacta taccttatcg ggcattcgta gaaaaattaa tagaaatttc acgctcagtt
    22441 accaaacttt ggaatgggac aacgaatgga aaacagtcga aacaacaaaa aacgttactc
    22501 ttccggttac agagattact gtgccggctc ctttgtgtaa caccacattt tctttgcagg
    22561 gtgatcaatt tacatccgaa ccatctttgg gtattcagcc aatcggaata atttcttcaa
    22621 tgtacgctgc cgtttctctg gaatgtcata taaaaacctc taccacagca cgtacagaaa
    22681 aaaatgaaga tgaacgtcca acttcagatg ctttgagcgg ttcagctcca ttggaaatgc
    22741 ttttcaatgc cgaagtcaac aatgcaggaa ccatttttta caaatgggat atttataaag
    22801 acaatcagtt gcttgtttcc cgttccgata aagaacatcg ttatactttc acagaggccg
    22861 gcacttataa tgttaagctg acggtaaaca atgatttttg ttcgtcatcc gattcggtat
    22921 cgatttcagt gagcacttca gatcttcaag ttccaaatgt gtttactccc aatggagatg
    22981 gaatcaacga cgaattcagg gtggcttata aatcattgat tagttttgat gcgcgtattt
    23041 acaatcgatg gggaagaatg gtttatcatt ggaacgatcc acaaaaaggt tgggatggaa
    23101 ccataggagg gaaaaaagcg cctgtcggag cttatttcta tgtgataaaa gctgttggtg
    23161 ccgatggcat taaatattcc aaaaaaggcg acattaattt gttgagagga agagaaaatt
    23221 aaaatttaaa cttttccgtt ctccaaaaaa taatcggaaa aattcttgaa ttgtcctttt
    23281 caaccatcaa ttttttaatg ggttggtttt tccatcaaat acatactgca aacaccaaaa
    23341 gtaaattttt tgtactcaac caattcaaaa ccattgtttt gaagaatttc aatcatttcc
    23401 tttcctttgg ggaaagcgtg catggaagag gtgaggtagc gataggcatt ttggtcggaa
    23461 gaaagatatt ttgccgtttg gtggacaaaa agtttgatgt aaagttggta tcctttcatc
    23521 aaccagcctt tttgaggttc attcagttca agtatcaata aatgacctcc cggtttcagc
    23581 acacgaaaaa tttgatgcaa gctttcttcc aatttttcaa aatttcgtac cccaaaagct
    23641 atggttacgg cgtcaaaaga gttgtcgtca aaattgagtt gagcgcagtc catcaccttc
    23701 agttttattt tatccgaaag ttgggcatgt tcaatttttt ctttgcctat ttcaagcatt
    23761 ttgggagaaa tatctatccc tgtaacttta tcgggattca gcaaccgaaa agccttgata
    23821 cacatatcgg cagttcctgc agccacatca agaatatgct tggggcggaa gcgctttagt
    23881 gtacttaatg cacgttttct ccataatttt gcaaaacccc acgacatcaa atcgttaaat
    23941 ttatcgtact gggcagctat ggtgtcgaac atgcgcacca attgttctgt ttttccttct
    24001 ttttcgttgt atggttttac ttcctgaaaa gtatattggc ttgtggtttt ttctttattc
    24061 atttgatttg taatttgttt gcaaaattaa ttacaaattt cggtaatcaa agcatttcat
    24121 tttatcaaaa aaagaaccaa agaaatttta cttatctatg aatctatcag tttcttaaaa
    24181 gtaaaattta aattttcgtg aaaagttgat gaaaaaaatt ttgtgttttg accttatttt
    24241 gtaattttgc aatcgattat tggtgtttat tttttgttct gaaaagtaaa caaaagggaa
    24301 tccggtgaaa atccggaaca gtacccgctg ctgtaagctc ctttataacg tactgaacaa
    24361 tactttggcc actttttcca caaaaagaat ctattttggg aaatgggaag gcgttcagat
    24421 acgggagcaa gtcagaagac ctgccaaaat catttttgct tttttgcttt cgggaataaa
    24481 tggcaaagaa agtcgaaaca tatctccctg attgagaata tattttttca tctgatgtgt
    24541 ttttcttctt ttttattttc atgaattttc cttaacaaat ttagcaagtt tttgtagtcg
    24601 aattgtttaa ttttaaaatg gaataaaaat gaaaaagaaa agtttgattg ttttatctac
    24661 attattgttg atttttttta cttcttgcga aaatgattcg gactttaagc tcaagaccac
    24721 tgtagatttt gaggatgttg agctgggaga aaatggttat tggaatggat ccgatttttc
    24781 gaagaaattc gtttccaacg gttttatttt tctgaacgat ttcacttcat cgccctgggg
    24841 cgatttttgg tcggggtttg cctgctcatc tttaaccgat accattactg ccggttacga
    24901 taatcagtac agtgtggctg ccggttcagg tgcatggggc tcaaataagt ttgctgttgc
    24961 ctttgatgat tcggccactg tgaattgtcc gaaaaatgaa ttgggaactt acgaagcaaa
    25021 aagtatcatg ctttgcaatt caacttatgc atatctcgat atgcgcaatg gatcggcttt
    25081 tagtaaaaaa tttggcgatg gagactggtt taaggttacc ataaaaggct ttttgaataa
    25141 taatgctaca ggtgcggttg attattattt ggccgatttt cggaacggga aacattatct
    25201 ttgtcatcaa tgggtaaaag ttgacctttc ttctcttgga aaagtagatc agttgatttt
    25261 tcttttcgat tccagcgata aaggaacttt tggagtgaat acaccaaaat atgtttgcat
    25321 agataatctt gaatttgagc aagatgctaa aaattaattt ttcaaaagac tttcaacgta
    25381 tgatattgct tttacctgcc ataatatcag catttcatac ttttgagacc gggtgtaaaa
    25441 tcccggtttt tttctttttg gaagtttgac ttttagagaa attgctcctg tttttgaaat
    25501 gaatgatgtg cagcattaaa atattctact tgaaaaaact cttcaatagt ttaataaaat
    25561 tcaattcttt tgcaataaaa tagataaatt caaaaactta tcgctatttt tgcatagtac
    25621 aatagtgaaa aaatggttat caaataatta aatgattgat aatcaaaaat taattttgta
    25681 tccaatcaag taacacctat ttataaacga catggaatat acaactttgc agggaaaact
    25741 gatcatgcct gaatatgggc gtaatattca gcaaatgata gcttatgcca tgacgataga
    25801 agacagggaa gagcgaaccc gttgtgtaaa aaccattatc aacattatgg gaaatttgtt
    25861 tccatatctg cgcgatgtga acgatttcaa acataaattg tgggatcacg tggctatcat
    25921 gtccgatttt aaactggata tcgattttcc ttatgaggtt cttcggcctg aaaatcttta
    25981 tacccgtccc gaaaagattc catataaaaa ttcagatatt cgatatcggc actatggccg
    26041 cacgttggaa gaaatgatag aaaaagtagc cgattatccc gatggagaag aaaaaaacga
    26101 attaatacgc ctgatagcca atcagatgaa aaaatgtttc cttacatgga ataaggaagt
    26161 agttgatgac cgaaaaattt tcgacgattt gcgtgaatta tccaaagaaa agctcgatat
    26221 ttcggaagat tttctgaagt tggttgaatc gaaagaagta ttacacaaca agaaaaataa
    26281 aaagaaaaac aaatagtttt cttttaccct tttccttttt ttaatcatca accaaccaca
    26341 aattatatgt cgtcatttca aatagaaggc ggtcatcgac tttcgggatc aattacaccg
    26401 cagggagcca aaaatgaagc gttgcaggtt atttgtgcaa ccttgcttac atccgagtca
    26461 gtcattattc gaaacattcc cgatattctt gatgttagcc atttactgag tctgttaaaa
    26521 gatttgggtg taaaaatttc aaaaatagaa aagggaactt attcgtttaa agccgactcc
    26581 ctaaacatgg actatttgca atcggaagat ttttatgaca aaagttcttc gctgcgtggt
    26641 tcggtgatga ttttgggccc gcttctggct cgatttggaa aagcgttggt tccaaaaccg
    26701 ggaggagaca aaataggacg tcgacgtttg gatacccatt tttcgggcat tcagaagctg
    26761 ggtgccgatt ttcgatacaa tgaaacaaaa aaacagtatg aagtatctgc aaaaaaactg
    26821 aaaggcacct acatgctgct ggatgaagcc tcggtaaccg gaacggcaaa tattttaatg
    26881 gcttcagtgc tggccgaagg taaaactaca atttacaatg ctgcctgcga accttatatt
    26941 caacagcttt gcagaatgtt gatagcaatg ggtgcaaaga tttctggagt tggctccaat
    27001 ttattgacta ttgaaggagt gaaacagttg aatggctgcg aacacactat tttgccggat
    27061 atgatagaag tcggcagttt tatcggcatg gcagccatga ccggttccga aattaccata
    27121 aaaaatgtgg catacgatga attgggcatt attcccgaca gttttcggcg aatgggtatt
    27181 caagtgcttc agaaaaatga tgatatttat attccggctc acgaaagcta taccatcgaa
    27241 acatttattg atggctccat aatgactatc gctgatgcgc cctggcccgg actgacaccc
    27301 gatttgttga gcgtttttct ggtaattgct gcaaaagcga acggaagtgt tttgattcat
    27361 caaaaaatgt ttgaaagcag attgtttttt gtcgataaac tgatcgacat gggtgcgcag
    27421 attattcttt gcgatccaca tcgggcaacc gttatcggac tgggaaatca gcttcgttta
    27481 cgggcatcgg taatgagttc gcctgatatt cgggcaggaa ttgccttgct tatagctgca
    27541 cttgcggccg atggaacaag tattattcac aatattgacc aaatcgacag gggctatgag
    27601 catattgata cccgtttgaa cgctttggga gcaaatatcg agagaatcca attttaaaaa
    27661 tttcatatac ttaatttaag ggaggaaata ttatgctgaa tgataaaatt accgaaatgt
    27721 tgaacaatca aatcaacaag gaattttttt catggtatct gtatttggat atttcaaatt
    27781 attacgccaa caaaaatttg gacggatttg ccaattggtt ttctattcag gcgcaggaag
    27841 aatatgctca cgctcagctt tttctaaagt atcttttaaa taatggtgaa aaggtgaagc
    27901 ttttacctat tgcagctcct gaaagctcat tcctcgattt caaaacacct ttgtcggctg
    27961 cgtttgaaca cgaattgttt atcacagctt ccatcaacga aatttatgcc gaggcatacg
    28021 ctcagaaaga ttttaaaacc atgcagtttc tcgactggtt tgtgaaggaa cagggagaag
    28081 aagaaaaaaa cgtggacgat ttgtgcaaac ggttcgacct tttcgggacc gatccacgtg
    28141 gtttgtattt gcttgataac gaattggctt cccgtgtata tactgctccg tcgctggtaa
    28201 tttaaagaaa tttttttctt tctgctgaag ttaaaccact ccaatcaaat gagttaaggg
    28261 attattttgc aacaaaaaat tttctttatt acgttgaaac ttatcggaat tttcacctca
    28321 accagaatat aggtttgggg ttttgctgat ttactctttt gacttttaag gatggcttaa
    28381 tcattcaaaa tcgacaaatt gtagtataga cagataggta ttggttaaaa ttagaaggct
    28441 tggcaggtat taacgatttg atatcgaatg gttttcttct gttagaaaaa aacacaacaa
    28501 tttggttcat agtaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncatct
    28621 tattatttgg aaaaacagat gctctgtcca tttttcacaa caatgatttc actcattcaa
    28681 ctgatatagt atttgggatt gtaaaaatga aaaaacacat tgttttttag aatatgaaca
    28741 gatgaaatac ttctatagga acatacaaga aaaaggatga agactgtcgt caattacttt
    28801 gtagaagaaa acctatggaa catatctaac gcaaaagcct tattactatc gacagagtta
    28861 cctctggatt tagcagaaag aaatgcataa cattagaaga atttaataaa aaaatgaact
    28921 tgaattggtt ttggttatta aaacggtttg gaagtgagcg tagaactaaa tctgtaaatt
    28981 gctaaattat cccgtacatt tgtaaaaacg atttttttga aactgtgaga atcatgaaaa
    29041 atattttgtt cgctgttgtt tttgtttttt tattcgttaa ttcgagttta acagccgcca
    29101 atccctatcg aaccaaaatt ttgaaaccca atatcaaaac tttacaaatc agaatttcgg
    29161 gtaccgactt cggtttcccc attttgaaat taaatggaac agaaacactt actgtggagt
    29221 ttgatgaaat gtctcacaat gttcgctctt ttggttacac ggttttgcat tgcaatgcag
    29281 actggacggt ttccaattta aacagcaacg aatatgtcga tggttttaca cgtggtaata
    29341 ttgacgatta cgccttttcg caaaatacta ctttccaata tattcattat cggtttacga
    29401 taccgaacca agacatgcgt tttaaaattt ccggaaacta tgtggtactc atttatgaag
    29461 acaatcgtgt ggatgatccg gtagccacag cctgtttttc cattgttgaa ccaaaagtag
    29521 agatagatgc ccgcgtcaga gccaataccg atatcgaatt gagtggacgc tatcagcagc
    29581 tcgacataga agtgaatctg gcaaattatg ttgttcagga tcctgcttcg gaagttaaac
    29641 ttgttgttcg acaaaataac aggttcgata acgaggtggc aaacataacg ccgacttttt
    29701 tcaatgccgg gaagttaagt tttgtcaata acaaagcatt gatttttgaa ggaggaaacg
    29761 aataccgtca ttttgatatt tcgtcttttt atgcagcagg agagggggtg gacagaatta
    29821 tttttaatcg caagtattat gaagcttttc ttaaggaaga tttgatgcaa aagtcgcgta
    29881 cctatatttc gcaacctgat gccaatggcc gatttatcat aaattttcag gaagcttttc
    29941 aggacgtaca aacggaagcc gattatgttc aggtacattt ttctttactt tcggaacaac
    30001 cgttttttga tggacaggta ttcattggtg gcgattacaa ttataatttg ctggatgata
    30061 cagcgcggat gcaatatgat ttcgatgctc aacgttatac caaaacgttt ttgctgaaac
    30121 agggcggcta cagttaccaa tactggtttt tgttaaaagg taaaatttcc gcttctgccg
    30181 gaaaaattga aggcagtttc tggcaaacta aaaacgaata taccatttat ttttaccatc
    30241 gggcttgggg tgaacgttac gaccgattga tcggttacag cgtaatcgac tgattataat
    30301 ggttggacaa aacattttat tttgtaacga tggttttttg caagcaacac cagttttttt
    30361 ggatgatgga ggttttttga agtttaattt tattgaccta cctttgcata agggaaattt
    30421 catcagaata taatttattt tttaaaggtc tgatggaacc cacctgtctg aatattatat
    30481 tcattcgtgt ttgtgtatgc aatacatgtg ggattcatat ttccgaaaaa cgataaatat
    30541 aattgtttgg aaagcaatta attatttttt actaagttaa atatcagatt tctacatgaa
    30601 tcaggaagac acatttccga caaccgactc cctaaaagat tttcaacaga attttaccaa
    30661 agaaccggca atcgaaaacc tgaaaaatgc tttaaacaat gaaattttca acattatttc
    30721 ggatattgcc gatgaagcac atcaggaatg ttatgtaatt ggcggtttcg tgcgcgattt
    30781 gctgctgcat cgaccatcaa aggatattga tattcttgtg gtgggcaatg gtattgaatt
    30841 ggcagagaaa gtggtcaatc aactcaagca actgaacaag ccttgcaaat ttgccatttt
    30901 taaaaatttt ggtacggcgc aaatcaaact gaaggaaatg gatgttgaat ttgttggcgc
    30961 ccgcaaggaa tcgtatcgtt ccgattcgag aaaaccgatt gtggaagatg gaactttgca
    31021 ggacgaccag aatcgccggg attttaccat caacaccctt gccctgtgcc tgaataaaag
    31081 tcgtttcggt gaattagtcg atccattcaa cggtctcagg gatttgcaaa acaaagttat
    31141 tcgcacccct ttgaatcccg atattacttt ttccgacgat ccgttgcgaa tgatgcgatg
    31201 tattcgtttt gcgacacaac tcaatttttt tattgaaacc aatacttttg aagccattgg
    31261 tcggaataag aagcgcattg aaattatttc gaaagaaaga attacagacg aattgaacaa
    31321 aattattctt tctcccaaac cttctatcgg atttattttg ctcgacaagt cgggtttgtt
    31381 ggatttgatt ttacccgaac tttcggccct caaaggtgta gaaaccaaag atgggattgg
    31441 gcacaaagat attttttatc ataccctcat ggtgttggac aatctcagtc agacttcgga
    31501 ttcactttgg ctgcgatggg ctgctttgct tcacgatata ggaaaaccgc gcaccaagcg
    31561 ctttgacaaa cgtcagggct ggacttttta taatcataac atagttggtg aaaaaatgat
    31621 agccgatatt ttcagaagaa tgaaacttcc caccaacgaa aaaatgaaat atgttcagaa
    31681 aatggtttcc ctgcacatgc gtcccattgt actcagtgaa gaagaggtta ccgattcggc
    31741 cgtcagaaga ctgctttttg atgcgggcga cgacattgac gacctaatgc tgctttgcga
    31801 agccgatatt acctctaaaa atccggaaaa ggtaaaacgt tacagggaaa attttcagct
    31861 ggttcggaaa aagttgaagg aaatagaaga aaaagacagg ataaggaatt ttcaacctcc
    31921 cattgatgga aaagaaatca tggaaacttt tcatcttcct ccctgtgctt tggttggaga
    31981 aattaaaatg aaaattaagg atgccattct ggatggtatt attcccaatg aatatgaagc
    32041 tgcacgaagc tatatgatgc agattgccgc agaaatggga ttaaccaatt aaaaacgtta
    32101 taaaaatgaa aagatttttt tattttattc cgctgttgct tttgtcctgt gcaataatgg
    32161 caaaaaataa tggaaatcct cagaaattta agcattccga aagaagtttt gctgctttcg
    32221 ataccgaacg tttggctcgg ttggacactg tttttcagga tttggtaagg aaaggcgaaa
    32281 ttcctcatgc tgttactttt gtggcacatc gtggcaatat tgttcatttc aaagcatttg
    32341 gctggcgcga taaggagaaa aatattccat gccaaagaga cgatattttt cgtatggctt
    32401 ctcaaaccaa agctattacc gcaacaggtg tgctgatgtt gatggaagaa ggaaaaattc
    32461 ttttggatga tccggtaaaa aaatatattc ccgaatttgc ccatccccaa gttctggtgt
    32521 cgtttaacga aaaagattcc acttatacta ccaggcctgc aaacaaggat attaccattc
    32581 gtcatttgtt gacacacaca tcaggaattt cttatggaac agattccaat aaccctatga
    32641 tgaaaatttt tgctaaaaag ggaattccac ccttgaacag cttggatccg gttacattga
    32701 aagaagtggt gaaaaaactt gcttcctgtc ctttggagca cgatcccgga gaaagattta
    32761 cctatggaat gagcgttgac gtgttgggtt atttgattga agtggtttca aaaatgcctg
    32821 ttgaccaata ttttaaggag aaaatttttc agccgcttgg aatgaatgat acttattttt
    32881 atgttcctaa ggaaaaacaa aatcggatgg ttacgcttta tcaaaaagtg cagggcaaac
    32941 ctttgcagcc cagttcaagt gtgctttatc agacatttcc atacagtggc gctcaaactc
    33001 tgttttcgac cggtgccggc ttgtcgggaa cgattgaaga ttatgcaaaa ttttgtcaaa
    33061 tgattctcaa caagggagaa tttaacggga aacgcatact gggaagaaaa acggtcgaat
    33121 tgatgtgtcg caatcaggtt ggagatttac gcggagaaat tggctttggt ttggcattcg
    33181 acgattttcg tcctcaatat ttgcatctta caatggcttc cgaaggttct ttacgctggg
    33241 gtggcatgtt cggaaccgat tatcttatcg atccgaaaga agatttgatt ttactttttt
    33301 atatcaattg gcaaaccgat aacagtgtcc ccgatgtcaa agttttgttc cacaatttgg
    33361 tctatcaggc attgaaatga ttgcactata ttttataatt ttacagtaaa atgaaagcag
    33421 ttattatcct ttctttttcc gttgttttta tggcagcttg tcattctttt tcaaaaattg
    33481 cttatccaca tacccaaaag gaaaatgtaa ccgatcgtta ttttggtact gatgttgccg
    33541 atccatatcg ctggctcgaa aatgatacgt cggcagcaac tgcagcttgg gtaaaagctc
    33601 agaatgaagt tactttcaaa tatctggaaa aaattccttt ccgaaatgcg ctgaaaaacc
    33661 gtctgactca actgaacgac tatccgaaat tgggtactcc gtttaaaaaa cacggcaagt
    33721 attatttttt caaaaataat ggattacagc cgcaaagtat tctttatcag cagtcaacct
    33781 tatattcgga acctgaaatt ttgctcgatc ccaacacttt ttcttccgat ggtacggttg
    33841 ccttgtcatc cgtttcgttt tcaaacgttg gcaattatat ggcttattcg gtgtcaaaaa
    33901 gcggttctga ttggaatgaa atttttgtct taaatctgga taaccgacaa actttatccg
    33961 atcacattca gtggacaaaa ttctggaatg ttgcatggaa agacaacgga ttttactata
    34021 tagcctacga tgctccgcaa aaaggcaagg aattgtcggc aaagaacgaa tttccaaaag
    34081 tttattatca ccagatagga caacttcaaa gcaaggattt gttgatttat caggataaaa
    34141 atcatccgtt gcgcgaattt ggcccatttg taacggaaga tgaaaaatat ctttttttga
    34201 tcattacgga aacaaccacc ggcaacgctc tgctgatgaa ggatttaacc gatcctaaag
    34261 cggaatttgt ttcattggtg gatggttttg aaaatgatta ttcggtcatc gaccacataa
    34321 acggcaaaat ttatattctt accaattgga aagcgcccaa taagcggatt atggaagttg
    34381 acccggccca tccttcgcgc gaaaactggc gtgaaattat tcccgaacaa aaaacggtac
    34441 tcgaatcggt tgaaattatc gatggaaagt ttttttgcct ttatctgaaa gatgctgctc
    34501 atcgtctgta tgcttacgat ttaagcggga aacttcttta cgaagtaaaa ttgccggcac
    34561 atggctcggt tgtaagcctg acgggaaaca aagacgacag tgaggctttt tttgctttca
    34621 gttcttttac ctatccacct accatttatc gcttggatgc cggcaagaat gtttccgaaa
    34681 tttttcaaaa agcgcaggtg aaatttaagc cggaagattt tgttaccgaa caggtttttt
    34741 atacggctaa ggacggtaca aaaattccca tgtttctttc ctatcgcaag ggaataaaaa
    34801 aagacggaaa aaatcctttg atgctttatg gttatggagg tttcaatatc agcctgacgc
    34861 cttctttcag tatttcgcgt attccttttc tcgaaaatgg aggtatttat gccgttgcca
    34921 acattcgtgg cggcggagag tatggagaat catggcacat ggcaggaaca aaaatgcaga
    34981 aaaagaacgt tttcgatgat tttattgccg cggcggaatt tttaattgca gaaggttata
    35041 cttcttcaaa aaaactggcc attaatggtg gctcaaatgg cggtttgctg gtaggagctt
    35101 gtttgacgca acgtcccgat ttgtttgcag tggccgttcc tgaagtaggt gtgctggata
    35161 tgcttcgtta ccatttgttt actattggct ggtcgtgggc aagcgattac ggtacgagcg
    35221 aagaaagcag ggaaatgttt gaatatctga aatcctattc tccgttgcat aatgtggtaa
    35281 aaggcacaaa atatcctgca gttctgatta tgacaggtga tcacgatgac cgtgttgtgc
    35341 cggctcattc attcaaattt gctgctactt tgcaggaagc caatgccggt aataagcctg
    35401 tattgattcg cgtggatagc aatgcgggac acggagcggg gaaacctttg ggaaaactaa
    35461 ttgatgcaca aacagatatg tggtcgtttg taatgtataa tttgggaatg aagcccaaat
    35521 ggtgaaaagg tttattaggt aaggaagtaa aaatcagatt tcaaaccatg taggaaaact
    35581 tatttttttc tgcgggtttt gaaaatggtc aaaagaagtc ctaaacccat gatgccggcc
    35641 aacaagtaac cgataacgcc caaaatggaa atatcggaaa tcagggggcc gattctttgt
    35701 tgcaaaatta acgaagaacc tacaatcagt gctgcaataa ttaaactggc cgatattctg
    35761 ttgctcgaac ttttcatttc atcttgcaaa ttttcgaggt tttgatgttc cagtttgatt
    35821 tcaaactgtc cctctttcaa ctttccaagc aaatcggata tattttgtgg aaaatgttct
    35881 attagttcaa tgctctcatc catcacgttc atgctgcgtt ttctgaaact ttttggagaa
    35941 tagagtttca gataaattct tttcacaaac ggttcaacaa tggcaaacat atcgaagtca
    36001 ggctccagtt gctgcccgat tgattcggca atagacatgg ctcgcagcat catcaccaaa
    36061 ttggaaggaa gcgccatgtc gtgacgagcc ataatttcca gcaactgctg gttcatccgg
    36121 ttcaggctaa tggttctgaa cggtctgtcg gcatacattt cgatcagttc ggtgaggtcg
    36181 tgccgcaatt gacgcttgtc ggtttcttta actacgattc caagttcctg taagcccttg
    36241 attacgtgat tgctgtcttt ttcgttgacg gcacgtaaaa ttcgggcaat ggtaatgatg
    36301 gattcttcgt cgagatgtcc cgtcataccc acatccaaca tcacgatggt ggcaggaggt
    36361 tggataaaaa gatttcccgg atgcggatcg gcatggaaaa aaccatctat cagaatttgt
    36421 ttcagcaatg cttttgccgc tctgtcggca acttttttgg gctcgtagcg gtgttggtat
    36481 ttttcctgtg tgatttcggt aattttcact ccttcaatga attccattgt cagtaatttc
    36541 tcggtagtaa attgccaata cacttttgga acttgtatgt aatccacatt tttgaaattt
    36601 ttcctgaatt tttcaaagtt gtgggcttca ttcatgaaat ccagttcttt tcgtatggtt
    36661 ttcttcagtt cggccacaat tgcagttgga tgatacaggg aactgttgcg aagtcttttt
    36721 tccagtattt cagcaaagct ttcgaggatg gagaggtctg tttcgatggt tttatgaata
    36781 tcgggccgtt gaattttcag ggctaccgta tcgccatttt gcagaactgc tttgtgcacc
    36841 tgacttaaag aagcggaagc aaccggcgat tggtcgaaat ccaaaaaaag ttcttcaagg
    36901 gtatgtttca gctctttttc aatacactgc ctcaccagcg gagtttccat aggtaaaacc
    36961 tgatcctgaa gtttttccaa ttcccggata tattccgcag gaagaaaatc gggacgggtg
    37021 ctgagtattt gcccaaattt tatgaaagtt gggcccaatt cttcaaaagc cagacgaagc
    37081 ctttcggcag tcgaaagttt atgataacct tttcgtcgtt tcctcaattt tgaaagaaag
    37141 ttcactcgag aagcttcaaa aagataatcc agaccaaatt tcacaaaaac ggaaatgatc
    37201 tgacggcttc gtttgacttt atccaagttg tttctgaaac cgaaattcct gtatggatag
    37261 gaatcgttca ttggtttatt tttccgattc ttcctgtttt ttgttgagca gttgcgtcag
    37321 ctcttctact ttgtttttca gttcttcgaa ttcttttcgt gaaggaatat tcattttttt
    37381 cagcaatgca tcgactgcct tttcaacctt ttcctccatt tcggttttag ttttttctga
    37441 ttgttccagc aaatctttga taaatttcgg tttctccgtc tcggttaatt ctccgcgttt
    37501 tatcaggtct ttagcgaatt cttcggcttt ttctcgcgta agcgaaacca atcccagccc
    37561 tgccaaaaaa gcatttttaa ttacgtctgc cataataata tcctcctgtt tttagaatat
    37621 tgatgatatt tttgtttgaa aaagttttaa ttggaaaaaa tgtcggtatc cgacttcaaa
    37681 cttaagatat tagttttgaa tttacaagat ttgtgctaaa aatttttatc ggttttattc
    37741 agcgatttcg aagcgttgtt tgatgaatga aacaaggtgg gccaacggca agaatttggg
    37801 attcggagca atgttagcgg taaattattc gatctacaat aattgattgt tggttggatg
    37861 aaaaagaaaa cttacagatt cctgtaacct gctatgtttc ttggaatagg tgctgatttt
    37921 ccaggtcgat ggaatttgca atttcatcca taaaaattat caggaattga attttgaaaa
    37981 aaataaacgt ttaagtaatt agcaatacta aattccaaat ccactgatta acattggtac
    38041 ggatgggaaa ttcttgcttc gaggattgtg cataacggct agcgcatttg cgatggcaag
    38101 gcttaaaatc accaaagctt tatgcctgca ctaagatgag aaaaagggca gaagttgaat
    38161 gaagaaattt cgccctgcta ttgctaatgc gctgttatag gcaggttttt accaatggat
    38221 ttaaatgaat attctttaca taattccgtg actttgtcat ttccaagtca aatagatcct
    38281 ttgtaacttc cactgaatga atacttttca gtccttccat tgttttttgt aacaataatg
    38341 atatcgccgc taattctggc tctatttgca ttatcggtta tagagccctg ataactccct
    38401 gatgttgagt atttctcaac tctgccattt gacttgacga ctacaactaa attttcgttt
    38461 gaatcagcat caatagcatt gtcagttata gtaccctgat agcttcctga agttgagtac
    38521 ttctcaactc tgcctttgtt tatttttaaa atcatattag acattttcaa aattttagtt
    38581 taattcctac ttgtaaggtc tttcggttac accccgttta ttaaagtggt ttctggtctc
    38641 cactttttgt ttttcaggca ggcaacactg tcaagaagtg tcaaaccgaa ttgtcggtgg
    38701 ttctcgtgtc tgtccgctat gccgtttttt aaacttgccc ataacttctt atttatgcca
    38761 taaatataca acatttgtaa ataaaaacaa taaattatga gaattatttt atgtttttgt
    38821 taatatgaat gataaatcac atattatcaa tacgatagac tgttgatttt tttataaaat
    38881 atttacattt acgaatgttt tgtaagtaac taacataaaa caatatcacc gaattctttc
    38941 tcacttgcat agtgagtcgt acattgatat agttgttgtt cggcttcaaa tataagacct
    39001 ctttcaacca tatgataagg aatacaattc aattttctgt aaaaatatgc tcttgtttat
    39061 gaatgatttt ttccacgata taattgtgcg ggagcagggg gaaagtattg ccctgtcctg
    39121 aaatcccact caccgctgct tgctgagaca aagtacctgc tgttgtttta tctttgtttc
    39181 ttttctcttt tcagaatatt tttatagtct tctgagagat atgcattata aaactcctct
    39241 ttgttcttaa aatattgtcc attcttattc cattccttgt ttaatgcttt attggtggca
    39301 atcacaaaat ctattacttg aggataatat gtcttatttt gactatcact tatcacagat
    39361 aatatgtttt ttcttatttc attcacactt gtataatcca tattagttgt aaacgtattt
    39421 gtggcaattc ccaaaaaaac ttttcccatt gtagaagcaa cagattcttt tttctccttt
    39481 tgaaagtcca ttgattctat gaactgaaaa ttactagtaa ggtaattaaa aatttttttt
    39541 ctctcaacct ctgatttttt tctctcaacc tccgattgat aatcaccttt tcctttctta
    39601 taaaaagtat caaattcgat cttgctttca aatagatttc cgtttttgct atactcattt
    39661 tcgtattctt gtatgatatc atccgttatt cgttcataac tgtattttat cgatgataca
    39721 cttttgtcat aattgtattt tctatcgttt ttgcctgtta ctttttgata atcgattatt
    39781 aactttttac ttagattata aggattttgt aataatttgg tgttacaatt attaaaaata
    39841 gaatccaact tttgattata agttgcaagt tccaacgaat ccatacgctc tagttcaagt
    39901 ttcttttttt gttccagctc gagcatcttt ttgcgagcaa tttgtgctaa ctgagacgga
    39961 ttttcccaag tatatgtttc atcatatgtt gatagtgaaa gcagcgccaa ctggtcgttt
    40021 tgaataaaaa aatgagcttt taatgcgttt acattctgaa tgttttgcca gttttttgcc
    40081 ttatcaagag gaattggtat cggtatattt ccccaaaaag tattgacaac tagcacttct
    40141 ttgtctgaat catattgtcc ttttgagtta taaaaaaaac cattaagcga agtgttatca
    40201 ttgtttagga cttccaacac ctgtacaaat tcatccaaag cagattcata aatttcagct
    40261 ttctttattg ttgtgctgtc gttcaatctt ttttgccagt ctgccgtttt ttcaaatgtg
    40321 cccttttttt tccaagtttc cagcttttgt tctacaaata cactgtattt gctgtttttt
    40381 accttgttta atctatccgc tttcacttgt tgtgcccact ttcctaatgc attgataaat
    40441 cctactgtat tattcgcttc acgcactgtt ttttctatat tagtttcggg acgaataacg
    40501 gttgtttgct tattttgcga aaaaacggta tttgcaaaag caaacacgat aaaagttaaa
    40561 aatatttttc tcataagata ttacggttat ttaattgttg attattgatt caataaatcg
    40621 ttcccaatcg ccatccacat ccaatataaa tacattttca gtcccaaaca cctctctaca
    40681 ttcttttgca aatggctcgt gcgtttcttt taaggataaa tacaagtatt gaaatttatt
    40741 tttttcgata agtttcctgt atttcttgta tttttctgtg tctcctttgc taaatattcc
    40801 cgaatgtttt atctctacaa ggtatttcct atctttatag aaaattgcta aatctgccgt
    40861 tcctctgctt ttcgtttcat cttaaatttc gaagcttgaa aatttatact gctgctcgat
    40921 ttttgaaatg ttgcttaatt cttcctgcaa acatttagcg acagtttgtt caaacaccca
    40981 gccttttagt aatttgtcaa cttcgagtgt ttggcaaaaa gacaaagaag ttaaggcttg
    41041 ttttgctttc tcgaagtttt tctcaaactc ttctttaatt tttttgttaa atcattttag
    41101 tagaacttta tacttgcgtc tatttcgcag accgttaatt ttattaatta attcctattt
    41161 tttgaaaatt tcatattccc cacaattgct ttcatgaatg atttgtgttt catctttgca
    41221 tcttattatt tcctgtattt gcttctcagg atcagcgcta taataaacat agtatgtagt
    41281 acagtcatca tatttaggat ttccagctaa aatgtaagtt attgtttctt cagaagtaat
    41341 aacctttatt tctgtttctc caaaaataat ttttacgttt aatgcttgcc attcatgact
    41401 tggatcttcc ccgtaataat aaagtttata atattctgtc tgttctccgt tgaaaaaaat
    41461 attttcttct tgccgttgtt tgttatcttc atttttactt tcgctcacag catactgaaa
    41521 agcatccgca agagcatctt cagcactgcg gggataaaat ctgatagaag aacccttagc
    41581 cagaccagaa tcataatctc cgaagtcctt tattaaaaca tttccaaaac cacagtagtt
    41641 aaacaagtat aaagtacccg ccaaagaaac aacgcaaatt ctgacaaccc acttgtaaac
    41701 cttagggtta gacttaacaa gaatcttatt gttcaaagca tttgggtctg gaacagaaac
    41761 acgaacagga ttgatgatac gtaacaaacc aataaacaac cccaacccac tcaaaacagc
    41821 agaaacaatt aaactccaaa caatagtgaa agaaatgtcc tgaatatccc aatttggaac
    41881 atctggtcca aaactgaaaa accacaacca ataacccaca cacaacatag gcaaaatccc
    41941 caaaaaaagc aaattggaaa tacctccatt agacaaagga acagaatgag tccaagaaac
    42001 ttccctctct accccattag agatctcctt tgaagtgtag gtaacaccgt ccttaaaact
    42061 atccgcataa gcaaaatcct cacgcttccc agcctttgaa gtgcgatatg cctccaactt
    42121 cttgaaacac cagtcgccaa aacctcccaa atgattgaga acagactcaa taacctcaaa
    42181 aacctcgtcg ctgcaagaac ctgcaaccat catcaaaact aacggcacac acagaataaa
    42241 agtgaccaat aaaataatcc attccataat ttttttgttt agagtccact ccttttgcag
    42301 actaaaaaat attaaatctc gtttttacgg cttctcaagt gccttgccta tttagaattg
    42361 gatacaattt cggggttctt acaaaggatt ttttgatttg ctatttttct tgttaccccc
    42421 atctcggtat ccgttgtcac tattagctgt cagttactgt ccttcgctgt gtcgcctgcc
    42481 acgtgcaccc aactttttgg cggtatgttt ttgttgccga
//
LOCUS       DAOSIB010000007        42458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_1061_length_42458_cov_12.764710, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000007 DAOSIB010000000
VERSION     DAOSIB010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 42458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42458
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1061_length_42458_cov_12.764710"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(861..1328)
                     /locus_tag="PLD44_01380"
     CDS             complement(861..1328)
                     /locus_tag="PLD44_01380"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HOF98023.1"
                     /translation="MIQRIQTLYLFLVLILSSVTLFSPVAVLFNKESATLYEISYKGL
                     CLLDESSKVPVSSVWALTAISAIVPVVTLIIIFLYNKRLIQIRLSIFNMIIMLGYYVI
                     LFIYLFYAAKTLHADWSLKIVSAFPLVNIILTLLAMRAIAKDEALIKSLNRLR"
     gene            complement(1395..2381)
                     /locus_tag="PLD44_01385"
     CDS             complement(1395..2381)
                     /locus_tag="PLD44_01385"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HOF98024.1"
                     /translation="MADNSLKKILKHYFKKIKTFFLSKDIFIFLLFFVFSAGLWFVNA
                     LGKERERIISIPLNYTGIPQHIAIVNNPPKSLSVKIKDEGMHLLQYRQKRLKPLDIYF
                     SQIFYEKGKILITPDQISNYLLRYLQPTTLVLETKPDSIVVEYEKLSQKLLPVECKIK
                     YSLAQQYMIVDKILLQPSKITVFGTAQQINSLKTLKTEPLLLSNLNDTVNTICRLQPI
                     HGVRFTTEAIQVFIPIEKFTEKKVRLPIQVLNVPNYITIRTFPTQVEATFNIALSRFS
                     SFDPAQIQLAVDYHQIKESASDKLQVFVIDHPSYVQNIRLSPQQVEFLVEKF"
     gene            complement(2391..2720)
                     /gene="yajC"
                     /locus_tag="PLD44_01390"
     CDS             complement(2391..2720)
                     /gene="yajC"
                     /locus_tag="PLD44_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOF98025.1"
                     /translation="MNLTAILLQATPAANGTGGGYTGILMMILIFVVFYFFLIRPQTK
                     RQKEIKKQRESMKVGDHVVTSGGIYGKIKEVKDNTMIIEIAENVRIKVDKNSVFLSPE
                     DIQSQAK"
     gene            complement(2797..3747)
                     /gene="nusB"
                     /locus_tag="PLD44_01395"
     CDS             complement(2797..3747)
                     /gene="nusB"
                     /locus_tag="PLD44_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOF98026.1"
                     /translation="MINRILIRIKIVQVLYSFYKNDEKSFTVAEKELFHSIEKTYDLY
                     FHLLQLAVEITRFANLRIDARKNKLRPSPEDLNPNMRFVENSFVEQLSTNKQFTKYLT
                     ERGLSWSDYPEVIKRLYEQITASDFYAEYMNAPFVDYEVDKNLWKKIYKKIILQSEEL
                     DSSLEEQNIYWINDIELVVSFIIKTIKKFQLSAGAQQELLPMFRDEEDVSFAAKLLKN
                     AISNCDEYRALIDKHTKNWELDRIAFMDIIIMQAALAELMEFPTIPVNVTLNEFIEIA
                     KIYSTEKSGTFINGILDNIVGQLKKENKLIKVVMFNENKK"
     gene            4014..4376
                     /locus_tag="PLD44_01400"
     CDS             4014..4376
                     /locus_tag="PLD44_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOF98027.1"
                     /translation="MEDVTRKEDLNKQPNEIVYSRAIKAGKRIYYLDVKKNKNDNFFL
                     AITESKKKVLGSDEDAQVSFEKHKIFLYRQDFEKFLEGLQDVMKFIRLQQPEEPYAES
                     HGKSSETTDEQDDFFLTK"
     gene            complement(4571..4753)
                     /locus_tag="PLD44_01405"
                     /pseudo
     CDS             complement(4571..4753)
                     /locus_tag="PLD44_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769314.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
     gene            complement(4834..5433)
                     /locus_tag="PLD44_01410"
     CDS             complement(4834..5433)
                     /locus_tag="PLD44_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98028.1"
                     /translation="MKKYIYLAFLCSFTLISCDENKEPAVTLKNEIKVKVFSTDTWDP
                     TTNKMDTVVGATVNLISDSGTSSVITDNKGIATFSNVTEDDYIIVAKKDNLCNLINTS
                     LEGDMTIGNLIIGVYTSEKDIEQSAFYANARVGGNKLADANGDGLINNLDKVRGQSSE
                     FEYQYKDLNGDGTIDAKDLSNGNLIQIDHQVEETIFIGK"
     gene            complement(5553..6812)
                     /locus_tag="PLD44_01415"
     CDS             complement(5553..6812)
                     /locus_tag="PLD44_01415"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOF98029.1"
                     /translation="MNATNHSYDWKEAALNAARTLQLVDDKAISETLLALADEIAIGS
                     EKILKANAEDLAAMEKAYPMYDRLLLNEQRLQNIAADIRNVAGLPSPLGKTLMETVRP
                     NGMKISKITVPFGVIGIVYEARPNVTFDVFSLCLKSGNSCVLKGGSDAYHSNAAIVSL
                     IREVLNRFGLNPDIITLLPSGHEATADLLNATGYVDLVIPRGGKGLIDFVRTHSKIPV
                     IETGAGICHTYFDEFGNTEKGSKIILNAKTRRVSVCNALDCLIIHEKRLSDLPALCKK
                     LAEKNVVIYADAKAYTFLVGKYPVHLLQKATQESFGTEFLDYKMAIKTVESLQEAIQH
                     INCYGSKHSECIVSENEANIALFDAMIDAACIYTNVSTAFTDGAQFGLGAEIGISTQK
                     MHARGPMALNELCTYKWIIKGNGQVRE"
     gene            complement(6882..7637)
                     /locus_tag="PLD44_01420"
     CDS             complement(6882..7637)
                     /locus_tag="PLD44_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOF98030.1"
                     /translation="MPEFENVLENREFQTLPSVSVDCVIFGYEGNLLKVLVRREYVPF
                     EGNILIEWKLPGNHVRRNEQIDATASRILKEQTGLENIFMKQFMVFSDPDRLKRRERD
                     YEWVKPRLQDDRVLTVGFYSLVNIHDVDNSKLLEEAKWENAYEIRELMFDHNDIFNEA
                     LKKLRYDLLHEPLIFELLPEKFTLTQMQKVYEAIFDTTYDKRNFRRKVNKMPYLIQTD
                     ELQSGVSHKPARYYTFDREIYENTRSERFDFRM"
     gene            7874..10186
                     /locus_tag="PLD44_01425"
     CDS             7874..10186
                     /locus_tag="PLD44_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOF98031.1"
                     /translation="MKNKRLNLFYLLLFFIPIFSAISAQESVSDRVEELLSKMTIEEK
                     VGQMAQITLDAIGNGATVFESKIPFAIDSVRLHKAIVDYHVGSVINTTNNYALSALQW
                     NSIIDQIQKTATTKTTLRIPVIYGIDAIHGVTYTSGATLFPQQIAIAATFNPGNAYNM
                     AKVTAYETRASGIAWNFSPVLDLGADPRFSRQYEGFGEDPYLASVMGRQAIRGYEGIT
                     DSIGHAEKVAACAKHFLGYSVPFSGKDRTPAYIPDHILREYHLPSFKAAIDEGVESVM
                     INSGIINGVSVHANYNIITKLLKEELGFQGVVVSDWMDIINLEKRDKIAKNNKEAIAL
                     AINAGIDMSMVPYDYEQFCKDLTALVKEGKVKESRIDDAVRRILKLKFKLGLFENPVT
                     KLSDYPDFGSDKHEKLAYKAAADAITLLKNKNNILPLNKNIKILVTGPNANSMRSLNG
                     GWSYSWQGEKTERYAAQYHTILEAVQQKIGEKNVLYEPGVSYVETGQYFEEKENGLQK
                     AVAAARKVDAIVLCLGENSYAEKPGDLNDLNLSELQIKLAKSLSAVNKPIILVLNEGR
                     PRCFSAIESKMNAIVQTYLPGNYGGDALADILFGDVNPSGKLPYTYPAYPNSIVNYYH
                     KFSEEQKPSEGAYKYESDYNPQYEFGFGLSYTTFAYSNLKISSSVLYPNELVEVSVDV
                     TNTGTREGKETVLLFTSDVIASITPDMRRLRRFEKINLKPNETKTVKFTLSANDLAFV
                     NTDNQWTIEPGEFEIKIGNLKKTINYQSTN"
     gene            10302..13214
                     /locus_tag="PLD44_01430"
     CDS             10302..13214
                     /locus_tag="PLD44_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006847815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOF98032.1"
                     /translation="MPLALSAQKTTITGVVVDSNNEPVIGATVLIKGTTTGTVTNYEG
                     KFTIEASSNDVLVVSYIGMVPQEIKVGNSKNINVVLKEDIKILSEVVVVGYGTQKKKD
                     LTTAVSTVSAEDFNVRPIVSAGQAIQGKAAGVQVIQPNGAPGGETVIRVRGTTSFNGN
                     SDPLYVVDGVPVDNINFLSPNDIENINILKDASSAAIYGSRAANGVVIITTKQGKSGE
                     SKIAFNAQLTAKNVTNKIESLNAADYKDLMEEVRPGSSSQMSSVDITNWYDEVYRTGL
                     QQSYQLSFSNSTEKTSYFLSAGYLDEKGIFPASFFKRYSFRANMTNEIRKWLSIGSNI
                     SYSDNSNNGITTGAGSNRGGVVLALINLPTSLPVINPETGYYNRSFYGQNITNPVEAM
                     ENGKDDITRENRLIASGNAMITFMKGMNFKSTFTLDRRHGLQTGFSPVIHVTGIDEYG
                     SAWDNRNMNTVLTWDNIFNYQKSFDKNNIELMMGSSWTDSKYTNSWINGSHYRNNKIK
                     TLNVANKISWNNTGTGGSTWGIMSYFGRVSYNYNSKYLITANFRADGSSRLHPDHRWG
                     YFPSFSAAWRISSEEFMQGLNWLDDLKIRGGWGQTGNQLGLGDYAYLQRYSVTRQQWW
                     ITGQENILPIITASNLRTTDLTWETTTQSNVGIDFTAFKSRLNIIMDYYYKKTTDMLL
                     NITLPSGANVTSIQRNGGLMRNTGFELSVNSINITNKNFKWTSDLEFSLNRNKLVKLD
                     LTKIYYDAATSDAFHGIQIVRNQEGRSLGGFYGYISDGVDSETGELIYRDINGDNKIT
                     ASDRTFIGDPNPDFIYGFTNTFTWKGFSLSIFLQGTYGNDIFNASRIETEGMYDLKNQ
                     STRVLDRWRRPGMITTIPKANFNLQPSSYFVEDGSYLRLKDLTLSYNVTAPFLKKSGI
                     SRLQPYITASNLLTFTKYLGMDPEVNEWGNSNAVQGIDWGTYPQAKSFVFGFNIEF"
     gene            13228..14778
                     /locus_tag="PLD44_01435"
     CDS             13228..14778
                     /locus_tag="PLD44_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOF98033.1"
                     /translation="MKIIKYILISAVVFVAASCSLDYNPIDTYSDITEGIDTTSAASQ
                     VILKDKAAVLNQRQVLYQRLKDGQEHWYLDMLLLAESHSDNAYAGSPNAETTPFEINS
                     IEGSNPNLKRDWNGHMDNIAQANRLIQGIDELKDPSLTEAEAKQIRAEGLIFRSLVYF
                     DMVRIWGRVPLITTVGKDITADNIEEVYPAYFPQQSEEIDVYRQIEEDLLEAVRYAPN
                     NDPSNKTLLTKSVARALLAKVYAEKPLRDYNKVIAYADSVAADGFELVSDFSDLWGVQ
                     LQNPSAPPGENNFAIDAKKRNTSEAILEIQYPVGSGNWVTWMFGRSLDNWNFWFDWAK
                     WITPSRDLINAFQAAGDIKRYNESVVWYSCSWNIYYPSNNYPFMYKTRSAYNSIIKIR
                     YADILLLKAEALIQTGDLSGAASIINDKIRNRAGLAPLPASVTNDKNALLSAYLNERR
                     LELAFEGQRWFDLVRLEKVEEVLNTLPSRDSGRPALRYPYDKYSYKLPIPQDIIDQNP
                     NLVQNPGY"
     gene            14798..16225
                     /locus_tag="PLD44_01440"
     CDS             14798..16225
                     /locus_tag="PLD44_01440"
                     /inference="COORDINATES: protein motif:HMM:NF027723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5016 domain-containing protein"
                     /protein_id="HOF98034.1"
                     /translation="MKNIIKLTIFGLLGLFATSCQQEELRVHYPASKPVFDSISVPET
                     NIVYGDSITLYVGVSDPLTPLSTLEIKVVVNDELIASETIRTKGNQATYQQRYGIPFV
                     ARMPDNAEVEIHLTSINVEGTKTDSIYVNTIAKRPTITDLYLVVPLKSPVKMKLTDAQ
                     NYIYTADNLSLENTVSFRLATKLKFGISIDWTGLVFGKVGDGIGLIDINGSPITLSDA
                     TLIGYKKITVDLFNFTVKGEGDKLQPATAMDISTFGTVSLSSTDYLNVTTKEDWKTKK
                     MYLGKGTEMTISGIQTTNLANNLAPDFFEVTGSNTAKFLGETGIYTVYYLPRLNFVFV
                     EQPDAVYPDAMWLCGVGFGPARTPHVKTSSWNWNTPLEYQFCRKVSDGVYQATFYALH
                     QIDDTAAEPWRLKFSVKFFHQRMWGGEEDARTYTLNVNGLLFAPTESDQGNFAGTDAF
                     KDKPGIYRLTIDVNNKTANFQKISD"
     gene            16283..17731
                     /locus_tag="PLD44_01445"
     CDS             16283..17731
                     /locus_tag="PLD44_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HOF98035.1"
                     /translation="MNKKSIFVFLFLLWAFIFFTGCKHSGDEPVPTPTPQDSVGDVSV
                     WLTTGNESCLLKKMAPINFSRDFADFTIVIDTTTTYQYIDGFGAALTGSSAHLIHQMS
                     AQNRKKLLDDLFDPEKGIGINYLRITIGSSDFSIGTYSYCDSPDINKFDIPEIDRNEL
                     LPVLKEILEINPSMKILASPWSAPAWMKSNNSMYGGSLKGEEVYNDFAEYFVRYVQVF
                     EQEGIHIDALTIQNEPMHETGGYPTMKMLWQEQNKIIRDYLGPKFIANGIQTKIVIWD
                     HNFDMSYYPINILNDPVTRQYVNGTGWHGYGGNASAIDQVTALYPDKETYFTEISGGG
                     WNTDTRMGNMFYYMKDFIMATVNRGSKNFLMWNLALNPQHGPTTTTWGGCQDCRGVVT
                     INENETYIRNEEYYLLGHFAKVVRLGAVRVNNSCYNLPSGMTISSFMNADGSKVVVIL
                     NRSGSSRKYNVRCGTRKFYYQQVDESVVSFKF"
     gene            17777..19150
                     /locus_tag="PLD44_01450"
     CDS             17777..19150
                     /locus_tag="PLD44_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98036.1"
                     /translation="MKIRALFFLMAFAIVTNCISAGKVTVWSSLPDTKALLVKKSMKL
                     NSKPVEIKNKVEIFPDQTCQSVEGVGAALTHSSAYVFHHNLTAQRRDSLFKVLFTPAG
                     IGINYTRLCIGASDFAFNLFSYSETEDFSLSNFNIQEDEKDVIPMLKEILAINPNLQI
                     LASPWSPPGWMKTSGSMVGGKLRPDCYDVYAEYLIKYIEAYRQHGITIHALTVQNEPE
                     YGTAAYPCMDMTAKEQQIFIRYFLGPKMQKKGIKTKIILFDHNCDNPDYPISILNDPN
                     TKKFVDGSGFHLYKGEISALCKVHEAHPDKNIYFTEQSGGGWAPDFDQNVRWYVGELF
                     AGAMNCWSKNVLLWNLALDENDGPKNFGCQNCWGVVRVTSDGRIEKNAEFYAIGHYGK
                     VVQPGAVRLVSNQDILKGVAFRNPDNSFAYLAVYYGTTPQTIGIQCGNSSFVYTFKPG
                     EVVSFLW"
     gene            complement(19473..20198)
                     /locus_tag="PLD44_01455"
     CDS             complement(19473..20198)
                     /locus_tag="PLD44_01455"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOF98037.1"
                     /translation="MLTENVFYIPQTVSTNVLCWKMLKERDIPEGFVVVADYQTAGKG
                     QAGNSWESARGKNLLFSIVLYPHHIPIEEQFLISQAVSVGIKNVLDTVSDDITIKWPN
                     DMYWKDSKLAGILIETSLFRGKISKAVIGVGLNVNQTEFFSDAPHPISLKQIAGKSFN
                     RKKLLHRIVASILEVYGQWESEKIRSVYAQSLYRKDGYNLYQAGTEIFRAKISRIYPD
                     GALELETEKGTSAKYYFKEVKFL"
     gene            complement(20209..21618)
                     /gene="dacB"
                     /locus_tag="PLD44_01460"
     CDS             complement(20209..21618)
                     /gene="dacB"
                     /locus_tag="PLD44_01460"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOF98038.1"
                     /translation="MKKIFFNVFVSFFVLNLVAQTPVDNFIHHPLLKNANVSLLIRET
                     GKGKVVGRYNANKSIIPASTLKLVTTASALELLGPNFRFQTKLEISGNVTKEHVLQGN
                     LIIKGSGDPTLGSEFLGEKNFLDQWVLAIKQAGISKIEGKIIADASIYNQEGVSPFWI
                     WEDIGNYYAPSIFGISYLDNTFRLFLRSGPIGTTPAIIRTLPDLPDLVIENYLKSSKI
                     TFDSAYFYGMPNSNLRRLYGEIPANRAEFVVKGDIPNPVFLLAKQFSDKLIQSGITVN
                     NPPVAEFSTPFDGQVVYTHFSPPLRDIIKQTNERSNNHYAECLFRYLGLGEGVPVTGR
                     GGVKTIRNLWKLKGLPVDQLFMCDGSGLSPMNAVSAQFITELLDYMYSKSAFSKDFFN
                     SLPIAGKTGTLVSFLAGTPLEGKVFAKSGSIEKVKCYAGYVKNEGKTYTFVVMVNNAQ
                     GTSRAVTKKIEELLLSVFK"
     gene            22069..24249
                     /locus_tag="PLD44_01465"
     CDS             22069..24249
                     /locus_tag="PLD44_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOF98039.1"
                     /translation="MKNFTFLLLFSVFGGELLCHASGEQANIISLKNDSLLFSSDQIE
                     LDVFDSIPEKNIKEVVVYGISSGRLTFPLSLVSKTSLLNSSFTTPADALQRETGIFMS
                     RDGIWATSVNIRGLSEQRLLFLVDEDRLLTATDIAAALSTVDMNALERIEVIKGAGSV
                     LYGTGAMGGVVNFVIERPAFTPFLESHGNIHSSFNSSNQLWSNHAKLQLTNRQWYLSL
                     NGSYRTADNIRTPEGKIPNSQFNDASWGMQAGMSSVANQELLVNYQHFEAWNVGLPGG
                     SAFPPTATVRYKNVQRNLFDAEYVFYEITDYLKQLRLKAYTQNISRDVENIVNTSTAI
                     FPSSNNITSGIKAVGDWEFTAYHKLTLGTEGWLRDAKTSRIKKVNSGTQINVFGEQPT
                     PNAQVQDMGIFAQYSWEFSPRYWMLDAGIRLDYMKTTNDSAFQPVYKYSYFPENPSMR
                     IYEDIPRSLLFKADEKNEFSYSAHIDLIYHPTRRQMLALSVSNSYRAPSLEELFKYID
                     QAGTLRVGNPDLRPEKGFFSNFSYTLSEDHFYIKTDLFANYLFDLIAEQHGTYTYQDE
                     KGKVTQREALINTNIDKALFLGGEMELQWKINPVFSVLANLSYTQGRNIDKDTFLPQI
                     PPMHGLFSVNYHQKELFDASVSALWAAKQGKVAPGENPTNGHLIFNADLHTEKFRINR
                     NYLRFFAGAENIFDKAYYNHLSTTRGVLRLEPGRNIYVKAELGW"
     gene            complement(24434..25018)
                     /locus_tag="PLD44_01470"
     CDS             complement(24434..25018)
                     /locus_tag="PLD44_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOF98040.1"
                     /translation="MKKNTIIIVIAALALFFLWGVSRYNKLVSAEEAVSSQWGNVENQ
                     YQRRADLIPNLVATVKGYAAHESQTLEAVTAARAKATQITVDPASLTPEKLQEFQAAQ
                     GELSSALGRLLMITENYPDLKANQNFIELQAQLEGTENRIAVERNRFNEIAKNYNATI
                     RRFPTSLIAAMFGFEKKPYFEAQEGSEKAPEVKF"
     gene            complement(25109..26422)
                     /locus_tag="PLD44_01475"
     CDS             complement(25109..26422)
                     /locus_tag="PLD44_01475"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOF98041.1"
                     /translation="MKKIFILLLTSFAICSIWSQTYTVETIPNPKKLNRFAFVSNPDG
                     ILNPETESQINNLLDSLEKKTGAEVAIVLVNSIGNEDIKSFAEQLFNSWKIGKEKEDN
                     GLLVLFVMDQRKVTFEVGYGLEGILPDAICKRIQIESMIPEFKKGNYDAGILAGVQRI
                     YATILKEPVQVEKTAPVNWKEILPLALGFYLIWLVLTWLWMGNAMNKVKMNPRLKSNM
                     ARYIAAKNERNGIVSVMAIFLPIAAFIAILVLSQAIYLLLLLPLPFMTIPPLIYANYR
                     LQKIRKEPFPCSVCGGQMHFVPEKEEDVYLKLSQQFEEQLHAVDYDVFVCDNCKNEAI
                     FTIDRPSAYSECPKCGTKAFILKDKKLTMAPTYFNAGTERLTYRCEFCGYEENKNRRI
                     PRLTRANSTFIGGAAAGSIFSGSGGFGGGSFGGGMSGGGGATSGW"
     gene            complement(26451..26906)
                     /locus_tag="PLD44_01480"
     CDS             complement(26451..26906)
                     /locus_tag="PLD44_01480"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOF98042.1"
                     /translation="MKPKSFFLFISTLLIFSFAGCKENNYEDWKIVNDQWMLKHQSDS
                     GFVVTSSGLCYQIIHQSVGRKPNASSWITANYKGYLIDGTLIEKGTYDYYLSSSISGW
                     QEGITKMRDGSTFIFYIPSSLAYGKDGSGSKIPPYSALIYEVTLVSSYY"
     gene            complement(27019..28392)
                     /locus_tag="PLD44_01485"
     CDS             complement(27019..28392)
                     /locus_tag="PLD44_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HOF98043.1"
                     /translation="MKSAKKFYLDASKIAFDEVHRKKIDFNMSRYDLAVETGMKRYKD
                     VEAAKNQAAAIKHEVLSHLDEYLLQFEKNISSHGAEVLWARNSEEAIAYVEKILVEND
                     ARLLVKSKSMTTEEIELNAAAAKLDCESVETDLGEFIVQTAGEKPYHIVTPAMHKSKS
                     EIADLFHEKFDTPPDSTPEQLSDFVRRLLRKKFTQAEIGVTGANFLIADIGGISVTEN
                     EGNALMSTSFPKIHIVFAGIEKIIPKMEQLGLFYPLLAVHGTGQQMTAYNTIFTGPRQ
                     TGETDGPEKMYVILLDNGRIGIFADDEAYQSLACIRCGACLNGCPVYRTIGGYTYNTT
                     YSGPIGSAISPFLKGFRDFYHLSYASTLCGKCAEVCPVKIPLPDILLANRRKAVEQHL
                     SPALETTVMKGFRLIASKRKGFDFFPAVMKNSAFYPLNFWGWGPERTVPKFAKKSFSQ
                     LYRKNNK"
     gene            complement(28413..29300)
                     /locus_tag="PLD44_01490"
     CDS             complement(28413..29300)
                     /locus_tag="PLD44_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98044.1"
                     /translation="MKKNNLILIVLSVVVLLLIGTVIFFVQVNKQKDAEMSEVVEMMN
                     FEKEQLEKDYSDLALEYDTYSSTIRNDSLVQLLQNEKMKVQQLLQELRITKATNARRI
                     AELRNELATLRTVMAQYVHQIDSLSAANKVLRVENVEVKRKYEAASETVKQLSKEKEN
                     LTEVVTRASMLEISNFSMTPLNSKNRKTGRYSQITTLQFNYTIQKNITCPPGMKTLYL
                     RLTRPDGEVMTKDPDDVFFYENKYIAYSAKKDFEYAGEALSDVIYWKVDEILQVGTYR
                     ADFFVDGFRIGSFTFDIKK"
     gene            complement(29356..30660)
                     /gene="murF"
                     /locus_tag="PLD44_01495"
     CDS             complement(29356..30660)
                     /gene="murF"
                     /locus_tag="PLD44_01495"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOF98045.1"
                     /translation="MQLYDIFLQHPIITTDSRNCPENSLFFALKGENFDANAFAHSAL
                     EKGCAFAVVDEKEYAVDNRFILVDDVLKTLQQLAHFHRQQMDIPVLGITGTNGKTTTK
                     ELITAVLREKFNILSTQGNLNNHIGVPLTLLQLKPEHQLAVIEMGANHPGEIDQLCQI
                     AAPNYGLITNVGKAHLEGFGSFEGVMRTKGELYDYVFRHGKKIFIDKNNNFLREMAFS
                     AGFSTSERIIEYGLNIPNKEPAAISGEILENSVFLKMNCHTPEGTFIVETRLIGAYNA
                     ENILAAIAVGSFFGVENNAIQRGIENYIPQNNRSQLTITQKNKLIVDAYNANPSSMSA
                     AIKNFARMEGEPKTLIIGDMLELGKQSEEEHRNIILLLQQNGFKNVLLVGSQFQKVND
                     NYRTFQNVEDLIKYLESSPLQGQNILIKGSRGIHLEKVLPFL"
     gene            complement(30818..>31006)
                     /locus_tag="PLD44_01500"
     CDS             complement(30818..>31006)
                     /locus_tag="PLD44_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98046.1"
                     /translation="KTDDEGNSRQSRFDIQMGVGAGIKVNGVQLRVGYDWGLLDTNNS
                     ENYTLTRDEFKASIGFDL"
     assembly_gap    31007..31106
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(31271..31933)
                     /locus_tag="PLD44_01505"
     CDS             complement(31271..31933)
                     /locus_tag="PLD44_01505"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOF98047.1"
                     /translation="MKKSLKPVFILTGILYFYLPLSAQTYRIEAGYLNHVRSGSGLSS
                     TYFDGAQVGVSSDFALKNNFSYSVGGYYSFLYSFKEQLFPHDTYVRYTTYSHSLDIPV
                     RLNYTLTIKKVKLFAYAGPNFNIGLYQPQKTVSTLTPELTELVQEFQHVTIPLGTTHS
                     ELYNDNVLRRLNIQLGAGGGIQWRKYRLKSGYDFGLLDLYKSKTASLHQSGWFVSFGY
                     EF"
     gene            complement(32055..33173)
                     /locus_tag="PLD44_01510"
     CDS             complement(32055..33173)
                     /locus_tag="PLD44_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOF98048.1"
                     /translation="MNLYPKLILETLSHVRYPGKGQDIVSLGMVQDNIRIEGNKVSFS
                     LIFEKPNDPFVKSLVKAAEQAVLTYVGKEVDIKGNITAVFKKEIPTAKENSILPGVSN
                     IIAVFSGKGGVGKSTIAANLAVALASLGYKVGLLDADIHGPSVPKMFGLEDARPFLEE
                     VDGKELIVPIEKYGIKLLSIGFFVDPDNALVWRGSMASNALKQLITDAHWGELDYFIM
                     DLPPGTGDIHLTLVQTMGITGAIVVTTPQEVALADARKGINMFTNEKVNVPVLGLVEN
                     MAWFTPAELPENKYYIFGKEGGKRLAEEFNIPLLGQIPIVQSIRESGDAGKPIATEED
                     SILSVSFRQLAENVVAAVEQRNKIMEKTKKVEINHPRK"
     gene            complement(33195..33956)
                     /gene="trmB"
                     /locus_tag="PLD44_01515"
     CDS             complement(33195..33956)
                     /gene="trmB"
                     /locus_tag="PLD44_01515"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOF98049.1"
                     /translation="MSKNKLAKFAEMETFPHVFQFPAKIVRNEDSNFEMQGKWNEKFF
                     GNSNPIVLELGCGKGEYTVGLAEIFPEKNFIGVDIKGARMWRGAKYSFEKKIPNVAFL
                     RTRIEMLHYFFGENEISEIWLTFPDPQMKKITKRLTSSGFLKLYSRFTAENALIHLKT
                     DSNFLFTYTMELLKLNHFPIEFSTDNLYEIASDHPILSIKTFYEEQWIERGIPIKYIE
                     FKLEKRIEILEPEVEIEHDQYKSFGRRPKHETTKN"
     gene            complement(33974..34465)
                     /locus_tag="PLD44_01520"
     CDS             complement(33974..34465)
                     /locus_tag="PLD44_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HOF98050.1"
                     /translation="MNKTKKITTTSKFVLLLSITCLIVSCNRNDVYLQYRSLPTKGWN
                     KDSVLTFDVPITDTTSLYNVYVNIRHRGEYPYQNLWFFLRQATPLMDTLQKDTIECYL
                     ADQRGKWLGSGVGSVYEMPILYRQKVSFPRAGIYRYQIIQGMRDSILAGINDVGIKIE
                     KVK"
     gene            complement(34566..35648)
                     /gene="ricT"
                     /locus_tag="PLD44_01525"
                     /pseudo
     CDS             complement(34566..35648)
                     /gene="ricT"
                     /locus_tag="PLD44_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773373.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
     gene            complement(35752..36903)
                     /gene="holB"
                     /locus_tag="PLD44_01530"
     CDS             complement(35752..36903)
                     /gene="holB"
                     /locus_tag="PLD44_01530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOF98051.1"
                     /translation="MLFSQVIGQEEVKKRLIRTVKEQKIPHAQLFYGPEGIGKLPLAI
                     AYAQYICCEHPLENDACGVCPSCVQFGKLAHPDLHFVFPIIKSEKKKLLVCDDFMDSF
                     RQIFAERIYFSQNDWYQKIYGEAKQGMIYTSEGDEIIRKVNLMTYESKYKVILIWLPE
                     RMNDQCANKLLKILEEPPENTVFLLVSNSHEEIIATILSRTQQVKIPRLSNEEIAEAL
                     VSRYQISENEAANAAQISEGSFLKALALIDENNVNRENFEHFVNIMRLAWIVGNKGDY
                     ASLKKIRQWSEEMAAASVGREGQKKFLSYAQRMIRENFIFNLHQPELNFMTSFEKEFS
                     VGFAPFINETNAEDMMNEFALAERHIEQNVNSKMVFFDLALKITKLLKK"
     gene            37432..39876
                     /locus_tag="PLD44_01535"
     CDS             37432..39876
                     /locus_tag="PLD44_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOF98052.1"
                     /translation="MAPKKPVKNGGRTTSGKVQKKSEKKSSGSGFGKKIASFFTDERT
                     HFIVGFMLLLVMLYLMLAFVSYFFTGAADQSKIDLSWSELHDKRTLIQNWTSVTGAIA
                     AEHLINRCFGVSAFFIPIFGIWVALRLMKAHEKSVWKAFFHCAFWLVWLSVTLGLTLS
                     PYFSDVLFFSLAGGHGDYAAQWLVSYIGASGSFLLMGGSFIVYLVIASASSLPFFRKL
                     FGKKNVSHTSSEETLVEPAVAVGTEIVPEEWISSGIETEVYEEFSKNQEESQTEKVAF
                     EVETTENNEESKPVDLDGNGENESLQEDPYYNLAKLGKYDPTLDLSHYHFPTLDLLKV
                     YGTDNETPIDMAEQIANKNRIINTLLNFDIEIETIKATVGPTITLYEIVPKAGVRISK
                     IRNLESDIMLSLAATGIRIIAPIPGKGTIGIEVPNNDPQIVSMHSIIASKKFQESKFE
                     LPIAFGRTITNEIFMVDLVKMPHLLVAGATGQGKSVGLNAIITSLLYKKHPSELKLVL
                     VDPKKVEFNIYAGIEKHFLAKLPDGSEAIITDTSKVVETLNSLCGEMDMRYDLLKKAH
                     ARNIKEYNEKFILRHLNPEKGHRFLPYIVVIIDEFGDLIMTAGKEVEMPIARIAQLAR
                     AVGIHMIIATQRPSVNIITGIIKANFPARVAFRVSSMMDSRTILDAPGANQLVGRGDM
                     LFSQGNDLIRVQCAFVDTPEVENIVHFISEQQSYPTAFYLPEYVKAEGEMGVASADLS
                     KRDPLFEEAARLIVANQQGSTSLIQRKFSIGYNRAGRIVDQLEIAGIIGPNEGSKARQ
                     VLIPDEYHLEQILKQL"
     gene            40051..40665
                     /locus_tag="PLD44_01540"
     CDS             40051..40665
                     /locus_tag="PLD44_01540"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOF98053.1"
                     /translation="MLLSIISIQPALSQNDPEAQRIIDSFLNTARTGAITTRFTVSVI
                     EKNGVNSYTNSGTFTLKGNRFVLEMQEMKVWFDGKTQWAYQKSGNEVTITEPSEQELA
                     QTNPMAILGNYRNKCNIRFCKTSSEYWCIQLTPKSIKNEDIAEIEVQFLKTDGIPQSI
                     TVSQKNGTRTVLMLSQFQKNAKVSEQIFTFDAKKYKDAYINDLR"
     gene            40692..41636
                     /gene="trxB"
                     /locus_tag="PLD44_01545"
     CDS             40692..41636
                     /gene="trxB"
                     /locus_tag="PLD44_01545"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOF98054.1"
                     /translation="MTTNEKIRCLIIGSGPAGYTAAIYASRANLSPVLYQGMEPGGQL
                     TTTNEVENFPGFPEGITGPELMDKMQKQAERFGADIRFGMVTSTDLSASPYRVTIDNE
                     KIIEAQSIIIATGATAKYLGLPDEQKYAGSGVSACATCDGFFYRNRPVAVVGGGDTAC
                     EEALYLANIASKVYMIVRKPFLRASKIMQERVLQHPKIEVLFEHETLGLTGEKVVEGA
                     DLVKRRGQPDEEKVHIAIDGFFLAIGHKPNSEVFKPWIETDENGYIKTIPGTPRTNVE
                     GVFAAGDVADPHYRQAVSAAGSGCQAAIEAERYLVDKK"
     gene            complement(42249..42395)
                     /locus_tag="PLD44_01550"
                     /pseudo
     CDS             complement(42249..42395)
                     /locus_tag="PLD44_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217920.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT        12774 a         8094 c         8191 g        13299 t
ORIGIN      
        1 ttcgctcgat aaaggggtta cttcgcgaaa agcattaata ttttccataa taaaaatatt
       61 ttgaaaacaa ataaaagtaa cttactttat agcaaaaaaa ttgatttatt actacttttg
      121 ttactttaaa caagtaaaga tacaaaaatg aaacattaaa atacattgtt gtatatagat
      181 taatataaat taatatacat caaagaatat taacattaca ttttgtgtga ttgctgaatc
      241 aaatcagaaa cgcaactttt tggttaaacg aatttcatca gaatataatt tattttttaa
      301 aggtctgatg gaacccacat gtctgaatat tatatttatt cgtgtttgtg tgagcaatac
      361 atgtgggttt cagaaaaaat caagtggagt taaaaattta tctaagtaca tgaatgaata
      421 aatttaaaat tatggatatt tgactgaaaa ccaaaattta aaatccgtgt tgcgatgaaa
      481 agaagatcat acttaatatg actttttccc gcttactgat ggtggcagag gaagtatgaa
      541 aaattagacc taattgaaaa tttgtaaaat aactttcttt aatttacttt ctaatgtgaa
      601 catttgttac ggcattcgaa tgataaacct gttgcataat tttttggttt aattttgaat
      661 aaattaatca agtttttcat tcgtatgttc ttttttaaaa ttatgttgct ggttaacgga
      721 gcgtgctttg gagtatgtcc aacgccattt cgtaacccac cgatactgct aaattcacac
      781 ttctatatag aacttagctc tcaacttttt aatttacaca cttcgtgaaa tagtgtccaa
      841 tttcctgtct ttacacaatt ttatctgagc ctattaagtg atttaattaa agcttcatct
      901 ttggctatag ccctcatggc caacagggta agaatgatgt ttaccaatgg aaatgccgaa
      961 acaattttta aactccagtc tgcatgaagt gttttggctg cataaaataa ataaataaac
     1021 agtattacat aataacccag catgatgatc atattgaaaa tagaaaggcg tatttgaatc
     1081 aatcgtttgt tgtaaagaaa gatgataatc agggttacca ccggaactat tgcactaatg
     1141 gcagttaatg cccaaaccga agaaacaggt acttttgaac tttcatccaa taaacacaag
     1201 cccttatagc tgatttcgta cagtgtggca gattccttgt taaagagcac tgcaacaggc
     1261 gaaaaaagag taacggaaga aagaattagc accaaaaaca aatataaagt ttgaatgcgt
     1321 tgaatcataa atttcgattt aagtggtttt gaaaagtaaa tttacgatga tgaaaacatg
     1381 aaaataaaaa attattaaaa tttttcaacc aaaaactcta cttgctgagg agaaagtcga
     1441 atattttgga cataggatgg atgatcaatg acaaaaacct gtaatttgtc ggaagctgac
     1501 tctttaattt gatgataatc tacagccaat tgaatttggg caggatcaaa ggaactgaac
     1561 ctgcttaagg cgatattaaa agtagcttcc acttgagtag ggaaagtacg tattgttata
     1621 taatttggta cattcaatac ttggataggc aaacggactt ttttttcggt aaacttttct
     1681 atcgggataa aaacctgaat ggcttccgtt gtaaaacgaa ccccatgaat gggttgaagt
     1741 cgacaaattg tatttacagt atcattgaga tttgaaagaa gaagaggctc tgtttttaac
     1801 gttttcaagc tgttgatctg ctgcgcagtc ccaaaaacgg ttattttgct tggttgaaga
     1861 agaattttat cgactatcat gtattgttgt gccaaagaat atttgatttt gcattctaca
     1921 ggcaacaact tctgactaag cttttcgtat tccacaacaa tagagtcggg tttggtttcg
     1981 agcaccagcg tagtaggctg aagatagcgt aaaagatagt tactgatttg atcgggagtg
     2041 atcaaaattt ttcctttctc gtaaaaaatt tgagaaaaat aaatgtcaag aggcttcaat
     2101 ctcttttgcc gatattgcaa aaggtgcatc ccttcatcct taatttttac tgacaaagat
     2161 ttgggtggat tgttaacgat agctatatgt tggggaatgc cggtatagtt caatggaatt
     2221 gaaataattc tttcacgttc ttttcccaaa gcatttacaa accaaagtcc tgccgaaaac
     2281 acaaaaaaaa gcaaaaagat aaaaatatct tttgacagaa agaatgtttt tatctttttg
     2341 aagtaatgtt tgagtatttt ttttaagctg ttgtcagcca taataccctc ttattttgct
     2401 tgcgattgaa tatcttcagg acttaggaac acggaatttt tatctacttt gatacgaaca
     2461 ttttcagcta tttcgattat cattgtattg tcctttactt ccttgatttt tccgtaaatg
     2521 ccacctgaag taaccacgtg atcacctact ttcatcgatt cgcgctgttt cttgatttct
     2581 ttttggcgtt tagtctgtgg gcgaatcagg aagaagtaaa acaccacaaa aattaaaatc
     2641 atcattaaaa ttccggtata tcctccgcct gttccgttgg ctgcaggagt tgcctgcaat
     2701 aaaattgctg ttaaattcat agatctataa tttatttgtt atttaatatt tgatatggta
     2761 aagtgtttct tttttaatga gttgcaaata taagtattat tttttgtttt cattaaacat
     2821 taccactttt attagtttat tttctttttt taattgtcca acaatattat ccaaaatacc
     2881 attgataaaa gtaccacttt tttcggtact gtaaattttt gcaatttcaa taaattcatt
     2941 tagagtaaca tttacaggaa tagtaggaaa ttccatcaat tcagctaatg cagcctgcat
     3001 aataatgata tccataaagg caatcctgtc caattcccag tttttggtat gtttgtcgat
     3061 cagcgccctg tactcatcac aattgctgat ggcatttttt aaaagtttgg cagcaaacga
     3121 tacatcttct tcgtctctaa acatgggtaa aagttcctgc tgagcacctg ccgaaagttg
     3181 aaacttttta atggttttaa tgataaaact gacaaccaat tctatgtcgt ttatccagta
     3241 aatattttgt tcttccagcg agctgtccaa ttcttcactt tgaagaataa tttttttata
     3301 aatttttttc cagagatttt tgtcaacttc ataatcaaca aagggagcat tcatgtactc
     3361 ggcataaaaa tcggatgccg taatctgctc gtaaagcctt tttattactt ccggataatc
     3421 cgaccaggat agtccgcgtt cagtcaaata tttggtaaac tgcttgtttg tagataattg
     3481 ttctacaaaa ctattttcaa caaaacgcat gttgggattc aaatcttcgg gtgaaggacg
     3541 caatttgttc tttcgggcat ctatgcgtaa attggcaaac cgtgtgattt ctacagccaa
     3601 ctgtagcaga tgaaaataaa gatcgtaggt tttttcaata ctgtgaaaaa gttctttttc
     3661 agctacagtg aacgattttt cgtcgttttt gtaaaaagaa tataaaactt gaacaatctt
     3721 aatccgaatt aaaatacgat taatcataaa atcagtggac gcttataaat aacgcacaaa
     3781 gataagtatt ttttttgttc cctcaacttt tgctctgcag caaaaaatcg atcaactatt
     3841 tcagaaaaaa accaaaatat gtccctattt agagaaaatg agaaatttat ttgaataatt
     3901 aaaaaaaaat actgaaattt ggagaaaatt gacttcaact ataaaatttt attataaatt
     3961 tttatagtta tttgaaatac agtgttttaa tttaaaaaga gaggttgaaa attatggaag
     4021 acgtaacaag aaaagaagat ttgaataaac aacccaacga aattgtttat tcacgagcca
     4081 taaaagctgg aaagagaatc tactaccttg atgttaagaa aaacaaaaac gacaactttt
     4141 ttttggccat taccgaaagc aaaaaaaagg ttcttggcag tgatgaggat gctcaggtat
     4201 cattcgagaa acataaaatt tttctttacc gtcaggattt tgaaaaattt cttgagggac
     4261 tgcaagatgt aatgaaattt attcgtcttc agcaacctga agaaccttat gctgaatcac
     4321 atggaaagtc ttctgaaacc acagacgaac aggatgattt ttttttgaca aaataaattt
     4381 ttaggaaaaa acaaagagaa aattcaatag gaggatagct ttttcgttgt cctttttact
     4441 cgattaattt ttacaagtaa gagaaggtac atttatgaaa ataaccatgt gattaaactt
     4501 tttccgttat atgttatttt gcaatcaaat aaattaggaa gttaatgtaa tttcactctt
     4561 ccggatattt ttattctctt acttgcccat tccctttgat gatccatttg taggtacaaa
     4621 gttcgtttaa cgccattgga ccgcgagcgt gcattttctg cgtactgatg cctatttcag
     4681 cacccaatcc gaactgagcg ccatcggtaa aagcggtaga aacattggta taaatgcagg
     4741 ctgcatcgat cattgcatcg aataaaaaag agagattgct ttacgcgttt aaatcttagt
     4801 gcctttttgt cagaaggctg ccgaagatta cttttatttt cctataaaaa ttgtttcttc
     4861 aacttgatga tcgatttgga tcagattgcc gttagataaa tctttcgcat caattgttcc
     4921 atctccattc aaatctttat attgatattc aaattccgaa gattgacctc gcactttatc
     4981 gagattgtta atcaatccat ctccattggc atcggcaagt ttatttcctc ccactcttgc
     5041 atttgcgtaa aatgctgatt gttcaatgtc tttttcagaa gtataaacac caataatcaa
     5101 atttccaata gtcatgtctc cttccaaaga agtattgatc agattgcaca aattatcttt
     5161 cttagccaca attatatagt catcttctgt aacattagaa aaagttgcaa ttcctttatt
     5221 atctgtaatt acagaagatg ttccagagtc cgatattaaa ttgacagttg cgccgacaac
     5281 cgtgtccatt ttgtttgtgg taggatccca tgtgtctgtg ctgaagactt ttactttaat
     5341 ttcgtttttc aaggttactg ccggttcttt gttttcatcg cagcttatca aggtaaatga
     5401 gcaaaggaat gcaagataaa tgtacttttt cattttttta gattgttatt gtaaattaac
     5461 ttttaaatat tttgacaaag ataattataa gaacatgaaa taaatacatt tcaggaacat
     5521 aaattttctt tttgattaat ttttttaaaa atttattctc ttacttgccc attccctttg
     5581 atgatccatt tgtaggtaca aagttcgttt aacgccattg gaccgcgagc gtgcattttc
     5641 tgcgtactga tgcctatttc agcacccaat ccgaactgag cgccatcggt aaaagcggta
     5701 gaaacattgg tataaatgca ggctgcatcg atcattgcat cgaataaggc aatatttgct
     5761 tcgttttcgc tgacaataca ttcactgtgt tttgagccat aacagttgat gtgttgaatg
     5821 gcttcctgaa gggactcaac agttttgatg gccattttgt agtcaaggaa ctcggtgccg
     5881 aaactttctt gtgtagcttt ttgaagcaaa tgcacgggat attttcctac aagaaaagta
     5941 taggctttgg cgtctgcata tataactaca tttttttctg ccagtttttt acataacgct
     6001 gggaggtcgc tcaatcgctt ttcgtgaata atcaaacaat cgagcgcatt gcagacactt
     6061 actcgtcggg ttttagcatt gagaattatt ttgctgcctt tttctgtatt gccaaattca
     6121 tcaaaataag tatgacaaat gccggctccg gtttcaatta ccggaatttt tgagtgtgtc
     6181 cgtacaaagt cgattaatcc ttttcctcca cgtggaataa ctaaatctac atatcccgtt
     6241 gcattgagca aatcagctgt ggcttcgtgc ccagatggca acaaggtaat gatatccgga
     6301 tttagaccaa aacgatttag aacttcacga atcaggctga caatggcagc attggaatgg
     6361 tatgcatcac tgccaccttt cagtacgcag gaatttcccg atttcagaca gagtgaaaaa
     6421 acatcaaaag ttacattggg acgagcttcg taaacaatac caatcacgcc aaacggaacg
     6481 gtaatttttg aaattttcat gccatttggg cgaacagttt ccatcaatgt ttttcccaaa
     6541 ggactaggaa gaccggcaac atttcgaata tctgccgcta tattttgaag ccgctgttcg
     6601 ttgagcagaa gccgatcgta cataggatat gccttttcca tagcggcgag gtcttctgca
     6661 tttgctttta aaattttttc cgaaccgata gctatctcat cggcaagggc aagtaaggtt
     6721 tcggaaattg ctttatcgtc gaccagttga agggtgcggg cagcatttag ggcagcctct
     6781 ttccagtcgt aagaatgatt ggtagcgttc atcatcaaaa aattttatag gttgagagct
     6841 aaaagttaac tgaatgactg caaaatgaac atgcaaagag tttacattct gaagtcgaaa
     6901 cgttcactgc gggtattttc ataaatttcc cgatcgaagg tataatacct ggccggtttg
     6961 tgggaaactc cgctttgcaa ttcgtccgtt tgaatcagat aaggcatttt gttgaccttg
     7021 cgtctgaaat tgcgtttatc gtatgttgtg tcaaaaattg cttcataaac tttttgcatt
     7081 tgagtcagtg taaatttttc gggcaaaagt tcaaatatta gtggttcgtg cagcaaatcg
     7141 taacgtaatt ttttcaaagc ttcgttaaaa atgtcattgt gatcaaacat caattcccga
     7201 atttcataag cattttccca ttttgcttct tcaagcagct tgctattgtc cacatcatga
     7261 atatttacca gcgaataaaa tccaaccgtt aaaactctgt catcctgcaa gcgcggtttt
     7321 acccattcgt aatctctttc gcgtcgcttg aggcggtcag gatcactaaa aaccataaac
     7381 tgtttcatga aaatattttc aagcccggtt tgctctttca aaatacgaga agcggttgca
     7441 tctatttgct cgtttcgacg gacgtggttg ccgggcaatt tccattcgat gagaatattt
     7501 ccttcaaatg gaacatattc ccttcgcacc agtactttca gtaaattgcc ttcgtaaccg
     7561 aaaataacac aatcaaccga aacgcttggc agagtttgga actctctatt ctctaaaacg
     7621 ttttcaaatt cgggcatgat aaaaacaata taatttttaa cccgacaaag ttaagaaaaa
     7681 aagatatttt tcgctaaaaa attttttctc aaaaaattga aaatcacggt ttcaaaaaaa
     7741 aatttttgta cttagtgtaa aaaaaacaca tttttatttg gagaataaaa ttttagcctc
     7801 tatatttgtc cttgaaaatt tgttttagct acataaaaat ttaatcaatt atttttttaa
     7861 aactattaat atcatgaaaa acaaaaggtt aaatctgttt tacctccttt tattcttcat
     7921 tccgattttt tctgctatta gtgctcagga atccgtcagc gatcgtgttg aagaattgct
     7981 ttccaaaatg actattgagg aaaaagtcgg gcagatggct cagataactt tggatgctat
     8041 tggcaacgga gctaccgttt ttgaaagcaa aatacctttt gccattgatt cggtgaggtt
     8101 gcataaagcc atagtcgatt atcacgtcgg ctccgtaatc aataccacaa acaattacgc
     8161 tttgagtgcg cttcaatgga atagtattat tgaccaaatt cagaaaacag caacaactaa
     8221 aacaactctg cgaataccgg taatttacgg aatagatgct attcacgggg taacttacac
     8281 ctccggagct actcttttcc cgcaacagat tgccatagcc gccactttta acccgggaaa
     8341 tgcctataat atggcaaaag ttacagctta tgaaacacga gcttcaggca ttgcgtggaa
     8401 tttttctcct gtgctcgact taggggctga tcctcgattc tcacgacaat atgaaggatt
     8461 tggagaagat ccttatttgg cttcggttat gggaaggcaa gcaataagag gttatgaagg
     8521 aattaccgat agcattgggc atgcagaaaa agtagctgcc tgtgccaaac attttttagg
     8581 ttattcggtt cctttttcgg ggaaggatcg cacgcccgcc tatattcccg atcatattct
     8641 cagagaatat catttgcctt cctttaaagc tgccatcgat gaaggagtag aatcggtcat
     8701 gatcaattca ggaattatta atggagtttc ggttcatgcc aattacaata tcattaccaa
     8761 actgcttaaa gaagaacttg gatttcaggg tgttgtggta agcgactgga tggacattat
     8821 caatctcgaa aaaagggata aaattgcaaa aaacaataag gaagccattg ctttggctat
     8881 caacgcaggc atagatatgt cgatggttcc ttacgattac gaacagtttt gcaaggattt
     8941 gacagcgctt gtgaaggagg gaaaagttaa agaaagcagg atagatgatg ccgttcgtcg
     9001 aattttaaag ttaaaattca aactcggttt atttgaaaat cccgttacaa aattaagcga
     9061 ttatcccgac tttggttccg acaagcatga aaaattggct tacaaggctg ccgctgatgc
     9121 cattacgttg ctgaagaata aaaacaatat tcttccgttg aacaaaaata tcaagatatt
     9181 ggtaaccggt cccaatgcca acagcatgag aagtttaaat ggaggatgga gttattcgtg
     9241 gcaaggagaa aaaaccgaac gctatgctgc tcaataccat actattttgg aagctgtaca
     9301 gcaaaaaatt ggcgaaaaga acgtccttta tgagccgggc gtaagttatg tggaaacggg
     9361 tcaatatttt gaagagaaag aaaacggttt gcaaaaagca gttgctgcgg ctcgtaaggt
     9421 agatgccatt gtgctttgtt taggggaaaa ttcttatgcc gaaaagccgg gagatttaaa
     9481 cgatttgaat ttgagcgaac tgcaaatcaa attggccaaa tcgctttcgg cagtcaataa
     9541 acctattatt ttggttttga atgaagggcg tccgcgttgc ttcagtgcta ttgaatccaa
     9601 aatgaatgcc attgtacaaa cttatcttcc gggcaattac ggaggcgatg cattggccga
     9661 tattcttttt ggagatgtca atccttcggg aaagcttcca tatacttatc cggcttatcc
     9721 taattcgata gtgaactatt atcataaatt ttcggaagaa caaaaacctt ccgaaggagc
     9781 ttataaatat gaatcggatt ataatccaca gtatgagttt ggattcggac ttagttacac
     9841 tacatttgcc tacagcaatt tgaaaatttc ctcttcggtt ctttatccca acgaattagt
     9901 tgaagtcagc gtggacgtta ccaatactgg aaccagagaa ggaaaggaaa cggtactttt
     9961 gtttaccagt gatgtaatag ccagtataac accggatatg agaagattgc gacggtttga
    10021 aaaaatcaat ttgaaaccca atgaaacgaa aacggttaaa tttactctgt cggccaatga
    10081 cttggctttt gttaacacgg ataaccaatg gacaattgaa ccgggagaat ttgaaattaa
    10141 aataggcaat ctcaaaaaaa caataaatta tcaatcaaca aattaaccaa ctaatttatc
    10201 aatcaatgga aaatgtaaag aaaaattccg gaaaaaaaat cgtgtctcgg tatgttacgc
    10261 gatttttaaa aagatggttg cccttactgg tgattattgt tatgccgctg gcattgtcgg
    10321 cgcaaaaaac aacgattaca ggagttgttg tcgacagcaa taacgagccc gttattggtg
    10381 caactgtttt gatcaagggg actactacgg gaaccgttac caattacgaa gggaagttca
    10441 ccatcgaggc atccagcaat gatgtgctgg tagttagcta tattggaatg gttccgcagg
    10501 aaattaaagt gggaaattca aaaaacatca atgtagtgct gaaagaagac attaaaattc
    10561 tttctgaagt tgtagtagtg ggatatggaa cgcaaaagaa aaaagattta actaccgcag
    10621 tatctaccgt ttctgctgaa gattttaatg ttcgacccat cgtttcggca ggtcaggcaa
    10681 ttcagggaaa agctgcaggg gttcaggtaa ttcaacctaa tggagcaccg ggaggagaaa
    10741 cggtgataag agtacgtgga acgacttctt ttaatgggaa tagtgaccca ttatacgttg
    10801 tagatggagt gccggttgat aatattaatt tcctttcacc caatgatatc gagaatatca
    10861 atatacttaa agacgcttct tcggcagcta tttatggctc tcgtgcagca aatggagttg
    10921 ttattattac tacaaaacaa ggcaagtcgg gtgagtcgaa aattgcgttt aatgcccaat
    10981 tgacagccaa aaatgttact aataagatag aatctcttaa tgccgctgat tataaggatt
    11041 taatggaaga ggttcgcccg ggttcgtctt ctcaaatgag ttccgttgat ataaccaatt
    11101 ggtatgatga agtttaccgc accgggttgc aacaaagtta tcagctttct ttctcaaata
    11161 gtactgaaaa aacgagttat tttctttctg ccggctacct tgatgagaaa ggtattttcc
    11221 ctgcatcttt ttttaagcgt tactctttca gagcaaatat gaccaatgag ataagaaaat
    11281 ggctgagtat tggttccaat atatcgtatt cagataattc caacaatggc ataactaccg
    11341 gtgcaggttc aaatcgaggc ggagtagtgt tggctttaat taatcttcca acaagtttac
    11401 cggttatcaa tcctgaaaca ggatattata atcgtagttt ttatggtcag aacattacca
    11461 atcccgtaga agcaatggaa aacgggaaag acgatattac gcgtgaaaac cgattaatag
    11521 cttcaggtaa tgccatgata acttttatga aaggaatgaa tttcaagtca acttttacgc
    11581 tcgacagaag gcatgggtta caaacgggtt ttagtcctgt gatacacgtt acaggtattg
    11641 acgaatatgg aagcgcatgg gacaatagaa atatgaatac ggttctgaca tgggataata
    11701 tttttaatta ccagaaatcg tttgataaga ataatattga attgatgatg ggttcgtctt
    11761 ggactgattc aaagtacacc aatagttgga taaacggttc tcattataga aacaacaaaa
    11821 taaaaacgct taatgttgcc aacaaaatat cgtggaataa tacgggaacc gggggctcta
    11881 catggggaat tatgtcttac tttggacgtg tttcgtacaa ttacaacagt aagtatttaa
    11941 ttactgccaa tttcagggct gatggttctt ctcgtttaca tcccgatcat cgatggggat
    12001 attttccttc tttttcggct gcatggcgta tatcgtccga agaatttatg caggggctca
    12061 attggctcga cgacctcaaa atacgtggtg gttggggaca aacgggtaat caactcgggc
    12121 tcggcgatta tgcctatttg cagagatatt ccgttacccg tcaacaatgg tggataaccg
    12181 gtcaggaaaa tattttacca atcattactg ccagcaattt gcggactaca gacttgactt
    12241 gggaaactac aactcagtca aatgtgggta ttgattttac ggcatttaag agcaggttga
    12301 atattattat ggattattat tacaaaaaga ccaccgatat gttgctcaac attactttgc
    12361 cttcgggggc taatgtaaca agtattcagc gtaatggcgg attaatgcgc aatacaggtt
    12421 ttgaactttc cgtaaactca atcaatatca cgaataaaaa tttcaagtgg acttcagatt
    12481 tggaattttc gctcaatcgg aataaactgg taaagcttga tttaacaaag atttattatg
    12541 acgctgctac ttccgatgct tttcatggga ttcagattgt acgcaatcag gaaggacgtt
    12601 ctcttggcgg tttctacggc tacattagcg atggggtaga ttcagaaacg ggggaactta
    12661 tctatagaga tatcaatgga gacaataaaa taactgcgtc tgaccgaact tttattggag
    12721 atcccaatcc tgattttatt tatggattta ccaatacatt cacatggaaa ggtttcagtc
    12781 ttagtatttt cttgcaagga acttatggaa acgatatttt caatgcttca agaatagaaa
    12841 cggaaggaat gtacgatttg aagaaccaat cgacgcgtgt tctggatcga tggagacgtc
    12901 cgggtatgat aaccaccatt cccaaagcca acttcaatct tcaaccttcg tcctattttg
    12961 tagaagatgg aagttattta agattgaaag atttgacatt atcctataat gtaacggctc
    13021 catttttgaa aaaatcggga atctctcggt tacaacctta tataactgct tccaatttat
    13081 tgacttttac aaaatattta ggtatggatc ctgaagtcaa tgagtggggt aattcaaacg
    13141 ctgttcaggg aattgactgg ggaacatatc ctcaagcaaa atcctttgtt tttggtttta
    13201 atattgaatt ctaaaaaatt tattactatg aaaataataa aatatatact gatttccgct
    13261 gtggtatttg ttgcagcatc atgctcgctc gattataacc ccattgatac ctattcggat
    13321 attactgaag gaattgatac cacatctgca gccagtcaag tgattttaaa agataaggct
    13381 gccgttctga atcaacgaca ggtactttat cagcgactga aagacggtca ggaacattgg
    13441 tatcttgata tgttgttgtt ggcagaatct cattccgata atgcttacgc cggatcccca
    13501 aatgcggaaa caacaccttt tgaaataaat tctattgaag gatctaatcc aaatcttaaa
    13561 cgtgattgga atggacacat ggataatatt gcccaagcca atcgacttat tcaaggcatt
    13621 gacgagttga aagatccttc cttgacggaa gcagaagcta aacaaattcg tgccgaagga
    13681 cttatttttc gctcactggt ttacttcgat atggtcagaa tttggggtag agtgcctctt
    13741 ataactactg taggaaaaga cataacggcc gataatatcg aagaagttta tcctgcgtat
    13801 tttcctcagc aaagtgaaga aattgatgtt tatagacaga tagaagaaga tttgttggaa
    13861 gcagtacgat atgcacccaa caatgatccg agcaataaaa ccctacttac caaatcggtg
    13921 gcaagagctc ttttggcaaa agtgtatgcc gagaaaccat tgcgtgatta caataaagtt
    13981 atcgcttatg ccgattctgt tgcagccgat ggttttgaat tggtttccga cttcagcgat
    14041 ttgtggggtg tgcaacttca aaatccttct gcacctccgg gcgaaaacaa ttttgccatt
    14101 gatgcaaaaa aaagaaacac atcggaagcc attcttgaaa tccagtatcc tgttggatca
    14161 gggaattggg taacatggat gtttggccgt tcgcttgaca actggaattt ctggttcgat
    14221 tgggctaaat ggataactcc ttctcgcgat ttgatcaatg cttttcaggc tgccggcgac
    14281 ataaaacgtt ataacgaaag tgtggtttgg tattcttgca gttggaatat ttattatcct
    14341 tccaacaatt atccatttat gtacaaaacc cgttcggcct ataacagcat tattaaaatt
    14401 cgttatgccg atattctgtt attgaaagct gaagcgttaa ttcaaaccgg agacttgagt
    14461 ggtgccgctt caattataaa tgataaaatt cgaaaccgcg cagggcttgc tcccttgcct
    14521 gcttctgtaa caaatgacaa aaatgcgttg ttgagtgctt atctgaatga acgtcgtctt
    14581 gaattggctt tcgaaggaca acgctggttt gacttggtaa gattggagaa agtggaagaa
    14641 gttttgaata cgcttccttc aagagattca ggtcgccctg cgctccgtta tccttacgat
    14701 aaatactcat ataaactccc tattcctcag gatattatag atcagaatcc aaatttggta
    14761 caaaatccgg gttattaata attaaaattc tattcaaatg aaaaatataa tcaaactaac
    14821 aatattcgga ctgctggggt tattcgcaac atcttgccag caagaagaac tgagagtgca
    14881 ttatcctgcc tccaaacccg tttttgacag cattagcgta ccggaaacca atattgttta
    14941 tggcgattcc atcacgttat atgtaggcgt ttccgatcct ctgactccat tatctacgct
    15001 cgaaataaaa gtggttgtaa acgacgaact cattgcttcc gaaacgatca gaacaaaagg
    15061 gaatcaggca acctatcaac aacgttacgg aattcctttt gtggcgcgta tgcccgataa
    15121 tgcagaagtg gaaattcatt tgacttccat taatgtggaa ggaaccaaaa cggatagcat
    15181 ttacgtaaat accatagcca aacgccctac tattacggat ctttatttgg ttgttccact
    15241 taaatcgcct gtcaaaatga aattgaccga tgctcaaaat tatatttata cggctgacaa
    15301 tttaagtctg gaaaatactg tttctttccg actggctacc aaattgaaat ttggaataag
    15361 catcgattgg acaggtttgg tattcggcaa ggtaggagat ggaattggat tgatcgatat
    15421 taatggaagt cccattactc tgtctgatgc aactttgata ggttacaaga aaattacggt
    15481 tgatctgttc aactttactg ttaaaggaga aggcgacaag ctgcaaccgg ctacagcaat
    15541 ggatatcagt actttcggaa ctgtttcgct ttcgtcgaca gattacctga atgttactac
    15601 caaagaagac tggaaaacga aaaaaatgta tcttgggaaa ggcaccgaaa tgaccatcag
    15661 cggtattcaa acgacaaatc ttgccaataa tctggctccg gatttctttg aggttacagg
    15721 ctcgaatacg gcaaaattcc tgggcgaaac cggcatttat acggtttatt atttgcccag
    15781 actcaatttt gtatttgttg aacagcccga tgccgtatat ccggatgcca tgtggctttg
    15841 tggggtaggt tttgggccgg cacgcacacc tcatgttaaa acatcgtcat ggaattggaa
    15901 cacgccgctc gaatatcagt tctgcagaaa agtttccgat ggcgtttatc aggcaacatt
    15961 ctatgcactt catcaaattg acgatactgc agcagaaccc tggagattga agttcagtgt
    16021 caaattcttc catcaaagaa tgtggggcgg agaggaagat gcccgcacct atactttaaa
    16081 tgtcaatggg ttgctctttg ctccgactga gagcgatcaa ggtaattttg ccggaaccga
    16141 tgcgtttaag gataaacccg ggatttatcg acttaccatt gatgtgaaca ataagacggc
    16201 taactttcaa aaaataagtg attaattatt tacatccatc ccgtaaacaa cttttttgcg
    16261 ggatggataa tttttttttg agatgaacaa aaaaagcata ttcgtatttt tatttcttct
    16321 ttgggctttt atttttttta cgggatgtaa gcattcggga gatgaaccgg ttcctactcc
    16381 tacacctcag gattcggtag gggatgtaag tgtatggttg accaccggca atgaatcttg
    16441 tctgttaaaa aaaatggcac cgattaattt ttccagagat tttgccgatt ttaccattgt
    16501 catcgacacc acaacaactt atcaatatat tgatggtttt ggtgctgcgt tgaccgggtc
    16561 ttcagcacat ttaatccatc aaatgagtgc ccaaaacagg aaaaaacttt tggacgatct
    16621 gttcgaccct gaaaaaggaa tcggtatcaa ttatttgcga ataacaattg gttcttccga
    16681 tttttctata gggacataca gttattgcga cagtcccgat attaataaat tcgatattcc
    16741 cgaaatagac cgaaatgaac ttttgccggt attgaaagaa attcttgaaa ttaatccatc
    16801 gatgaaaatt ctggccagtc cctggagtgc gccggcatgg atgaaaagta ataacagcat
    16861 gtatggcgga agtttgaaag gtgaagaagt ttacaatgac tttgcagaat attttgttcg
    16921 ttatgttcag gtttttgaac aggaaggcat tcatattgat gcgctgacca ttcaaaacga
    16981 gcctatgcat gaaacaggag gatatccgac catgaaaatg ctttggcagg aacaaaacaa
    17041 aattattcgc gattatttag gccctaagtt tattgccaac ggcattcaaa cgaaaattgt
    17101 gatttgggat cataatttcg atatgtccta ttaccctatc aatattctaa atgatccggt
    17161 tacccgacaa tatgtaaatg gtaccggctg gcatggatac ggaggaaatg cttcggccat
    17221 cgatcaggta actgcacttt accccgataa ggaaacttat tttaccgaaa tctcgggtgg
    17281 aggatggaat acggataccc gcatgggaaa catgttttat tacatgaaag atttcattat
    17341 ggctacggtc aatcgtggct ctaaaaattt tcttatgtgg aatctcgctt taaatcctca
    17401 acatggacca actaccacaa cttggggtgg ctgtcaggat tgcagaggtg tggtaactat
    17461 caacgaaaat gagacttaca ttcgcaacga ggagtattat ttgctgggac attttgctaa
    17521 agtggtacgt ttgggagctg tacgtgtaaa taattcctgt tacaatttac cttccggcat
    17581 gaccatatct tcctttatga atgccgatgg cagcaaagtg gtggtcattt tgaacagaag
    17641 cgggtcatct cggaaatata atgttcgctg tggaacccga aaattttact atcagcaagt
    17701 agatgaatcg gttgtatcat tcaaattctg atttttatcg agaaattatt ttttttgtta
    17761 aataaaaagt ttatccatga aaataagagc tttatttttt ttaatggcct ttgccattgt
    17821 taccaattgc atttcggcag ggaaagtaac tgtttggtca tcgctgcccg ataccaaagc
    17881 tttattggta aaaaagagca tgaagttgaa ttctaaacct gtagaaatta aaaacaaggt
    17941 tgaaattttt cccgatcaaa cctgtcaatc ggttgaaggt gttggagctg ccctcaccca
    18001 ttcttcagct tatgttttcc atcacaatct gacagcccaa agaagggatt cattgtttaa
    18061 ggtgcttttt actcctgcag gcatcggcat caattatacg cggctttgca ttggtgcttc
    18121 cgactttgcc tttaatcttt tcagttacag cgaaacggaa gatttttcgt taagtaattt
    18181 taatattcag gaagatgaaa aagatgtaat tcccatgctg aaggaaattt tggccatcaa
    18241 tccaaacttg caaattttgg catcaccctg gagccctccc ggatggatga aaacttccgg
    18301 aagtatggtt ggcggcaaat tgcgtcccga ttgctacgat gtgtatgccg aatatttgat
    18361 caaatacatt gaggcttatc gacaacatgg gataaccatt catgcgctta cggtgcagaa
    18421 cgaaccggaa tacggaactg cagcttatcc ttgtatggat atgacggcaa aggaacaaca
    18481 gattttcatc cgttattttc tgggccccaa aatgcagaaa aaaggaataa aaaccaaaat
    18541 cattcttttc gatcacaatt gtgacaaccc ggactatccg ataagtattt tgaatgatcc
    18601 caatacaaag aaatttgtag atggttcagg atttcatctt tataaaggcg aaatatctgc
    18661 gctttgtaaa gttcatgaag cacatcccga taaaaatatt tatttcaccg aacaatcggg
    18721 tggaggttgg gctcccgatt ttgatcaaaa cgttcgttgg tatgttggag aattgtttgc
    18781 cggagccatg aactgctgga gtaaaaacgt tctcctctgg aatttggctt tggacgaaaa
    18841 cgacggacca aaaaatttcg gttgccaaaa ttgttggggc gtagtacgcg ttacttcgga
    18901 tggaagaatc gaaaaaaatg ctgaatttta tgccattggt cattatggga aagtggtaca
    18961 accaggagct gtccgactcg tttcaaatca ggatatttta aaaggcgtgg cttttcgcaa
    19021 tcctgataac agttttgcct atctggccgt ttattatggc acaactccgc aaaccatagg
    19081 tattcagtgt ggaaacagtt cttttgttta tacttttaaa cccggagaag tagtatcttt
    19141 tctgtggtag atgtaaaaaa ttgctatcgg ttgcagttca gataaaaatc tgtccaagcc
    19201 gatagcaatt ttttgtgaat tttcgagcga aactttcgct gaatatcttt ctaaaagata
    19261 atttttttca agaaagcaat ttttttacca tttccgaaat cgctttccca tctgtttttt
    19321 cccctccagc tggcgagtgg aaattcccac tacttttcat aaattttgag cccctgtggc
    19381 accggtttgg gctatgattt atctgaaagt tactctctga aaatctcagc ttactttttt
    19441 acacctgtaa gtccaatttt gaaaaaacgc aatcaaagaa attttacttc cttaaaataa
    19501 tatttggctg aggttccttt ttcggtttcg agttccaaag cgccatccgg ataaatgcgg
    19561 ctgattttgg cccgaaatat ttctgtcccc gcctgataaa gattatagcc atctttgcga
    19621 tacaaggatt gtgcgtaaac acttctgatt ttttcggatt cccactgccc gtacacctcc
    19681 aaaattgacg caacaattcg atgcagcaac tttttccgat taaaactttt gccggcaatt
    19741 tgtttcagag aaatcggatg gggtgcgtcg ctgaaaaatt cggtctgatt gacattcaag
    19801 cctacaccta ttaccgcttt tgatattttc ccccgaaaaa gggaagtttc gatcagtatt
    19861 ccggccagct tgctatcttt ccaatacata tcgttgggcc atttgatggt aatatcgtcc
    19921 gaaactgtgt ccaagacatt ttttattccc acactcaccg cttgcgaaat cagaaactgc
    19981 tcttcaatag gaatatgatg cgggtacagt actatgctga aaagcagatt ttttccgcga
    20041 gccgattccc aagaatttcc tgcctgtcct ttccctgctg tctgataatc tgcaaccaca
    20101 acaaatcctt ccggaatatc tctttctttc aacattttcc aacacaacac attggtggat
    20161 accgtttgag gaatataaaa aacattttcc gtgagcatgc aaaacaattt atttgaaaac
    20221 cgacagcaac agttcttcga tttttttggt taccgctctt gaagttccct gcgcattgtt
    20281 taccatgacg acaaaagtat aggtttttcc ttcgtttttt acataaccgg cataacattt
    20341 cactttttca atgcttccac ttttggcgaa aactttccct tccaatggcg taccggccaa
    20401 aaacgaaaca agagttcctg ttttcccagc aatgggtaaa gaattgaaaa aatctttact
    20461 gaaagcactt ttggaataca tgtaatccag caactcggta ataaactgag ctgaaacagc
    20521 attcatgggc gaaagtcctg aaccgtcgca cataaagagc tgatctacgg gaagtccttt
    20581 caatttccac aaattacgta tggttttaac tccgcctctg ccggtaacgg gaacaccttc
    20641 accaagtccc aaataacgaa aaagacattc tgcataatga ttgttgcttc tttcgttggt
    20701 ttgcttgata atatctctca atggaggaga aaaatgcgta taaacaactt gcccatcaaa
    20761 aggcgttgaa aattcggcaa caggaggatt attaactgta atcccactct ggatcaactt
    20821 atcggaaaac tgctttgcca aaagaaaaac cggattggga atatcgcctt tcactacaaa
    20881 ttctgctctg ttggctggaa tttcgccata aagccgacgc aaattggaat tgggcatacc
    20941 gtaaaaatag gcactgtcga acgtaatctt tgaacttttt aaataatttt ctatcaccaa
    21001 gtccggcaaa tcgggaaggg tgcggataat ggcaggcgtc gtccctatcg gaccggaacg
    21061 taaaaacaaa cgaaaagtat tgtcgagata agaaattccg aaaatggaag gagcataata
    21121 atttccaata tcttcccata tccagaaagg actgactcct tcttggttgt aaatactggc
    21181 atcggctata atttttccct caattttact gattcctgct tgttttatag ccaacaccca
    21241 ttggtcaaga aaattttttt ctcccaaaaa ttccgaaccc aatgtggggt cacctgaacc
    21301 tttaataatt agattcccct gtaaaacatg ttctttggtt acattgccgg aaatctccag
    21361 tttcgtttga aatctgaaat tcgggcccag caattcgaga gccgaagctg tagtaaccag
    21421 ttttaaagta gaagccggaa taatgctttt gttggcattg taacgtccca ccactttccc
    21481 ttttcccgtt tcgcgtatca gaaggcttac attggcattc ttcaacaaag gatgatgaat
    21541 aaaattatcg acaggagttt gagcgacaag gttgagcaca aaaaagctaa caaaaacgtt
    21601 gaaaaaaatt ttcttcataa aataaaaatt tcagatcatg ttgatttata gataaatgta
    21661 tatctcactg taatgctgta aaaaataaat ggtaatagaa gtacattgtt tgccgtgacc
    21721 tgttctatag caatagttaa tctgcacaaa aacaaccaaa ttatgttccc aaaccatttc
    21781 aatatttatt tctcaataaa tttttatttt gtacaaattc gacccttatc ttgggtattg
    21841 agtcaaaagt agaagtttca aatgtacaat atttttttaa atttgtcatt ccttccggtg
    21901 aattaaaaat atagcaatta tcgtttcaca gcaaagagaa tgattttaat attttttcat
    21961 tgattaaagt tttgcttatt ttcaacttaa aaattgcgtc aagttagtgt tataattaaa
    22021 tttaatcttg taactttgcg aaggttcgtt atctttactg aaaaaagtat gaaaaatttt
    22081 actttcctgt tgttgttttc cgtttttggc ggggaacttt tatgccatgc ttcaggagaa
    22141 caagcaaata taatttcact aaaaaatgat tctttgcttt tttcatcaga tcagatcgag
    22201 cttgatgttt tcgattctat tcctgaaaaa aacataaagg aagtagttgt atatggcatt
    22261 tcttctggta gattgacttt cccactttcg ttggtaagta aaacatcact tctgaatagt
    22321 tcttttacca cccctgccga tgcgttgcaa agagaaaccg gcattttcat gtcccgcgat
    22381 ggaatttggg ctacttcagt taatatcaga ggtttgtccg aacaaagact tctttttctg
    22441 gtagacgaag atcgattgct aaccgcaacc gatattgcgg cagcattgtc cacagtggat
    22501 atgaatgctt tggaaagaat agaagtgatc aaaggagccg gctctgtttt atacggcacg
    22561 ggagctatgg gcggagtggt gaattttgtg attgagcgtc cagcatttac tccttttttg
    22621 gaaagtcacg gaaatattca ttcgtccttt aattcttcca atcaactttg gagcaaccac
    22681 gcaaaattgc agttaaccaa tcgccagtgg tatttgtcgt tgaacggaag ttatcgtact
    22741 gcggataata tacgtacacc tgaaggaaaa attccaaaca gtcagtttaa cgatgcttcc
    22801 tggggaatgc aagccggcat gtcgtcggtc gccaatcagg aattattggt taattatcag
    22861 cattttgaag catggaatgt cggattgccc ggcggtagcg cttttccgcc aacggcaaca
    22921 gttcgataca aaaacgtaca aagaaattta tttgatgcag aatatgtttt ttacgaaata
    22981 accgattatt taaaacaact tcgccttaaa gcttatacgc aaaatatcag cagagacgta
    23041 gaaaatattg tcaatacatc tacagcaatt tttccttcca gcaataatat tacttcaggc
    23101 atcaaagctg tgggcgactg ggaatttact gcataccaca aactgacatt aggaactgaa
    23161 ggatggttga gggacgccaa aacatcacgc atcaaaaaag ttaattcagg aacacaaata
    23221 aacgtttttg gagaacaacc cacacccaat gcacaagttc aagatatggg aatttttgca
    23281 caatacagtt gggaattttc gccacgttac tggatgttgg atgccggaat aaggttggat
    23341 tacatgaaaa ccaccaacga cagcgctttc cagccggtgt acaaatattc ttattttcct
    23401 gaaaatccat ccatgcgaat ttatgaagat attcccagaa gtttgctttt caaagccgat
    23461 gaaaaaaatg aattttccta ttctgcccat atcgatttga tttatcatcc cacacgtcgt
    23521 caaatgttgg cactttctgt ttccaattcc tatagggcgc cttctttgga agaattattc
    23581 aaatatatcg atcaagccgg aaccttgcgt gttgggaatc ctgacctaag gcccgaaaaa
    23641 ggcttttttt caaacttcag ctatactttg tcggaagatc atttttatat aaaaaccgat
    23701 ctttttgcca actacctctt tgatttgatt gccgagcagc atggaactta cacttatcag
    23761 gacgagaagg gaaaagttac gcaaagagag gcactgatca atacaaatat cgacaaggca
    23821 ctctttttag gaggagaaat ggaactgcaa tggaaaataa atcctgtgtt ttccgtgttg
    23881 gctaatttgt cctacacaca gggaagaaat attgacaagg atacttttct tccacaaata
    23941 ccacctatgc atggattgtt ttcggtgaac taccatcaaa aagaactttt tgatgcctct
    24001 gtttcagctt tgtgggcagc aaaacaagga aaagttgctc cgggtgaaaa tccgacgaat
    24061 ggccatttaa tttttaatgc cgatttacat acagaaaaat tcagaatcaa cagaaattat
    24121 cttcgttttt ttgccggtgc agagaatatt tttgataaag cctactacaa tcatctttcc
    24181 acaacacgtg gagtgctacg tctggaaccg ggaagaaata tctatgtaaa ggccgaattg
    24241 ggttggtaaa agagtttttt ctgaacgaat tatttcatta tttcacagaa aaatagataa
    24301 aagaggagta aaaaaatcat cgaaagctga ctttttactc ctcttgtttg aattttaatt
    24361 ttggtcttta ttattttcga aaatcagaga aaagattttt ctttgctttt catcagttca
    24421 aaaaaattct ctattaaaac ttcacttcag gagctttttc gcttccttcc tgtgcttcaa
    24481 aatatggttt tttctcgaaa ccgaacattg ctgctatgag ggaagttgga aaccgtcgaa
    24541 tggtagcatt atagtttttt gctatttcgt taaaacgatt acgttccaca gcaattcgat
    24601 tttctgttcc ttccagttgt gcctgcaatt ctatgaaatt ctgattggct ttcaaatcag
    24661 gataattttc ggtaatcatc agcaatcttc ccaatgcgct gctcaattct ccctgagcag
    24721 cctgaaattc ctgcaatttt tccggcgtaa gcgaggctgg atccacagta atttgagtgg
    24781 ctttggcacg agctgccgtt acggcttcca aagtttgact ttcgtgtgca gcataccctt
    24841 tgacggttgc taccagattt ggaattaaat cggcacgtcg ttgatattga ttttcaacat
    24901 ttccccattg gctcgaaaca gcttcttctg ccgaaaccaa tttgttgtaa cgggaaactc
    24961 cccataaaaa aaacagtgcc agagcggcga taacaatgat aatggtgttt tttttcataa
    25021 tttttttatt tagaaaatgt gtaattcatt gaaaaaagta gaaattgtta tagtttttga
    25081 aattgaagaa ttttaaatat gtcttatctt accaaccgct ggttgcgcct ccgcctcccg
    25141 acatgccgcc accaaaactt ccaccaccaa aacctccgct tccactgaaa atactgccgg
    25201 ctgctgctcc tccgataaag gtactgttgg ctcgggtaag tcgcggaata cgccggtttt
    25261 tgttttcttc gtagccgcaa aattcacaac gataggtaag cctttctgtt ccagcattga
    25321 aataggtagg ggccatagtg agttttttgt ctttcagaat aaaagctttg gttccacatt
    25381 ttggacattc tgagtaagcg ctgggcctat cgatagtaaa aatggcttca tttttacaat
    25441 tgtcgcaaac aaatacatcg taatcgacag catgaagttg ttcttcaaat tgttgggaaa
    25501 gtttcaggta aacatcttct tctttttccg gaacaaagtg catttgtccc ccacaaacac
    25561 tgcagggaaa aggttctttt ctgatttttt gaagcctgta attggcatat atcagtggag
    25621 gaattgtcat gaaaggaaga ggaaggagca aaagcagata aatagcttgc gataagacaa
    25681 gaatagcgat aaatgcggca ataggtaaaa atattgccat aacgctgaca atgccgtttc
    25741 tttcattttt tgcagcaatg tagcgtgcca tattactctt gagtcgcgga ttcatcttca
    25801 ccttattcat agcgttaccc atccacaacc aagttaatac caaccatata agataaaaac
    25861 ccaatgcaag cggcaaaatt tctttccaat ttacaggagc tgttttttca acttgaacgg
    25921 gttctttcaa aatagtggca tagatacgtt gaacgccggc cagtatgcct gcatcataat
    25981 ttcccttttt aaattcggga atcatgcttt caatttgaat acgtttacag atggcatcag
    26041 gaagaatccc ttccagtcca taaccgactt caaaagtaac tttccgttga tccattacga
    26101 aaagtacgag taaaccgttg tcttcctttt cttttcctat tttccacgaa ttgaagagtt
    26161 gctcggcaaa tgatttaata tcttcattgc caatagaatt gactaaaacg attgcaactt
    26221 cggcacctgt ctttttttct aatgaatcaa gcaaattgtt gatttgactt tcggtttcgg
    26281 ggttcaaaat accatcagga ttactgacaa aggcaaaacg attcagtttt tttggattgg
    26341 gaatcgtttc aacggtgtag gtttgactcc aaatggaaca tattgcaaag cttgtcagca
    26401 aaagaatgaa aatttttttc atttcctgat ttcttgaata aatttgtgtt tcaataatag
    26461 gaagaaacga gggttacctc ataaatcaat gcagaatatg ggggaatttt tgaacccgaa
    26521 ccatctttcc cataagcaag tgaactggga atgtaaaaaa tgaaagttga accatccctc
    26581 attttggtaa ttccttcttg ccaaccactg atggaactcg aaagatagta gtcgtatgtt
    26641 cctttttcga tcaatgttcc atcaattaaa tatcctttgt agtttgccgt gatccaacta
    26701 cttgcatttg gctttcttcc cacgctctga tgaatgattt ggtagcaaag tccactggat
    26761 gtaaccacaa atcccgaatc ggattgatgt ttcaacatcc actggtcgtt cacgattttc
    26821 caatcttcat aattattttc tttacatcct gcgaaagaaa aaatcagtag ggtcgaaata
    26881 aaaagaaaga aactctttgg tttcataagt cgattatttt tacgtttatt ggtaagaaaa
    26941 attggattca acattttttt ttaacaaaat gttttatatt tttcctcctt gcatatattt
    27001 gataatgaat aaattaaatt atttgttgtt ttttctgtag agctgtgaaa acgatttttt
    27061 tgcaaatttc ggaacagttc tttcgggtcc ccatccccaa aaatttaagg gataaaaagc
    27121 tgaatttttc attaccgcag ggaaaaagtc aaatcctttt cgtttcgagg caatcaaacg
    27181 aaatcctttc attactgttg tctccagcgc aggagaaaga tgttgctcaa ctgcttttct
    27241 cctatttgcc aacaaaatgt ccggaagcgg aatttttacc gggcaaactt ctgcacattt
    27301 tccacaaagt gttgaagcat agctcaggtg ataaaaatca cgaaacccct ttaaaaacgg
    27361 cgaaatggcc gagccaatag gcccactgta agtggtgttg tacgtgtaac cgccaatggt
    27421 tctgtacacc ggacagccat tcagacaagc gccgcagcga atacatgcca atgattgata
    27481 agcctcatcg tctgcaaaaa taccaattct cccattatcg agcaaaatta catacatttt
    27541 ttcagggcca tctgtttctc ccgtttgacg cgggcctgta aaaatcgtat tgtaggccgt
    27601 catttgttga ccggtaccat gaacggcaag caacggataa aaaagtccca attgttccat
    27661 cttcggaatt attttttcga tgccggcaaa aacaatgtga attttcggga aagaagtact
    27721 cattaaggca ttaccttcat tttctgtaac ggaaattcca ccaatatcgg caatgagaaa
    27781 attggcgccg gtaactccta tctcggcttg cgtgaatttt tttcggagca gacgacgaac
    27841 gaaatccgat aattgttctg gagtactatc gggtggggtg tcgaatttct cgtgaaacaa
    27901 atcggcaatt tcactttttg atttgtgcat ggcgggtgta acgatatgat agggcttttc
    27961 tcctgccgtt tgaacaataa attcacccaa atcggtttct accgattcac agtccaattt
    28021 tgctgcagca gcattcagtt cgatttcttc cgtagtcatc gatttgcttt tcaccagcag
    28081 gcgggcatcg ttttcaacca ggattttttc cacataagcg attgcttctt cgctgttgcg
    28141 agcccaaaga acttcggcgc catgagaaga aatgtttttt tcaaactgca acaggtattc
    28201 gtcgagatgc gaaagaactt catgttttat cgcagcagcc tggtttttgg cggcttctac
    28261 atctttgtag cgtttcatgc ctgtttccac agccaagtca taacgcgaca tgttgaagtc
    28321 gatttttttt cgatgaactt catcaaaagc aattttagag gcatcgaggt aaaatttttt
    28381 ggctgatttc atgacaataa ttcgagaacc ggttactttt tgatgtcaaa agtgaaactt
    28441 ccaattctga aaccatccac aaaaaaatcg gcacgatatg ttcctacttg aagaatttca
    28501 tctactttcc aataaattac gtcgctcaat gcttcgccgg catattcaaa atcttttttt
    28561 gccgaatagg caatatattt gttttcgtag aaaaaaacgt catctggatc ttttgtcatt
    28621 acttcaccat cgggcctggt cagacgcaaa taaagcgttt tcattcccgg cggacaggta
    28681 atgttctttt gaatggtata attaaactgt aaagtggtta tttgtgaata tctgcctgtt
    28741 ttccgatttt tgctattcaa aggggtcatg ctgaaatttg atatttccag cattgaagca
    28801 cgagttacca cttctgtcag attttctttt tctttggata actgtttcac tgtttcgcta
    28861 gcagcttcgt atttacgttt cacttccacg ttttctacgc gcaatacttt gttggctgca
    28921 ctcaacgaat cgatttgatg aacatactgg gccatcactg tccgcaaagt ggccaattca
    28981 tttcttaatt ccgcaattct ccgggcgttg gttgcttttg ttattctaag ctcctgtaaa
    29041 agctgctgca ctttcatctt ttcattctga agcagttgca ccaacgaatc attccgaatg
    29101 gttgacgaat aagtgtcgta ttccaatgca aggtctgaat aatctttttc cagctgttcc
    29161 ttttcaaaat tcatcatttc cactacttca gacatttcag catctttttg tttgtttact
    29221 tgtacgaaaa aaattactgt accgattaac agcaaaacga caaccgacag tacaataaga
    29281 atcaaattat tcttcttcat tcagatagac ataattaagt tgttaaatga tagaaattca
    29341 ttaaatgaaa taaacttata aaaatggcaa aactttttcc agatgtattc ctctggaacc
    29401 cttaataaga atattttgtc cttgcaacgg gcttgattcc aaatatttga ttaaatcttc
    29461 cacgttttga aaagtccgat agttgtcgtt gaccttttga aactgagatc caacgagcaa
    29521 aacgttttta aaaccatttt gttgaagaag tagaataata ttccgatgtt cttcttcgct
    29581 ttgtttacca agttcgagca tgtcgccaat aatcagcgtc ttgggttctc cttccatccg
    29641 agcaaaattc tttatggcag cgctcatgct cgaaggattt gcgttgtaag catccacaat
    29701 caatttgttt ttttgcgtga tggtcagttg cgaccgatta ttctgtggaa tataattttc
    29761 aatgcctcgt tgtatggcat tgttttctac accaaagaat gaacctacag ctatggcagc
    29821 caaaatattt tcagcgttgt atgcgccaat caatcgtgtt tcaacaataa aagtgccttc
    29881 gggtgtatga caattcattt taagaaaaac ggaattttcc aaaatttctc ccgaaattgc
    29941 agccggctct ttattgggaa tgttcaaacc atattcaata attctctcac tcgtcgaaaa
    30001 acctgcactg aaagccattt cacggaggaa attattattt ttgtcgataa aaattttttt
    30061 gccatgccga aaaacatagt catacaattc gccttttgtg cgcatgacgc cttcaaagga
    30121 cccaaaacct tccaagtgtg cttttccgac attcgttatc agcccataat tcggagccgc
    30181 gatttggcac aattgatcaa tctcaccggg atgattcgct cccatttcaa taactgccaa
    30241 ctgatgttcg ggcttcaatt gcaataaagt caatggcaca ccgatatgat tgttcagatt
    30301 tccctgcgta gaaaggatat tgaacttttc tcttaacact gcggtaataa gctcttttgt
    30361 agtggttttc ccgtttgtgc cggtaatccc cagaacagga atatccattt gttgacggtg
    30421 aaagtgtgcc agttgctgaa gtgttttcag tacatcatca accaatataa aacgattgtc
    30481 aacagcatat tctttttcgt caaccacagc gaaagcacaa cctttttcca acgctgaatg
    30541 agcaaaagca ttggcatcga aattttcacc tttcaatgcg aaaaaaagag aattttccgg
    30601 acagtttctg ctatcggtag taataatagg gtgttgtaga aatatatcgt atagttgcat
    30661 gaagtcaaat aaaatttttt aggctaattc atcacaaaaa tagtgataat cctctgaatg
    30721 aatggaaaca ttcaaatcat tttaacaaaa aaaggccaaa cacattcata gagttaagcc
    30781 tttttataga atgtagaaaa tttatttttc tcgcagttta gagatcaaat cctattgaag
    30841 ccttgaattc gtcgcgagtc aaagtgtagt tttcactgtt gttggtatcc agcaatcccc
    30901 agtcgtagcc cactcgaagt tgaacgccat taacttttat accggctcca acacccattt
    30961 gaatatcaaa acgcgattgc cggctgttgc cttcgtcgtc agttttnnnn nnnnnnnnnn
    31021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31081 nnnnnnnnnn nnnnnnnnnn nnnnnnaaca aaaaacttaa ctcttttcat actttttgat
    31141 tttaagttga taactgaatt ttagttaaga accaaaaaca aaaaatatac caatgagtaa
    31201 taaagaagtt tttacaagtt aaaaagtcga ttttagattc aggataagtt gtttacaaac
    31261 aatagtttaa ctaaaattca taaccaaaag aaacaaacca cccactttgg tgaagacttg
    31321 cagttttgga tttatacaaa tcgagcaatc caaaatcgta accactcttt aaacgatatt
    31381 ttctccactg aataccgcct ccggctccca gttgaatatt caaccttctc aatacgttat
    31441 cgttatacaa ttcggaatgt gtggtgccca gaggaatggt aacgtgttga aattcctgaa
    31501 caagttcggt gagttcgggt gttaatgtag aaacggtttt ttggggctga tataatccga
    31561 tattaaaatt tggtccggca taggcaaaca attttacctt ttttatggtc aaggtgtagt
    31621 taagtctgac cggaatatcc aatgaatgtg agtatgtggt atagcgaaca tacgtatcgt
    31681 gtggaaaaag ctgttccttg aaggaataca gaaacgaata atagccacct acagaataac
    31741 tgaaattgtt tttcagggca aagtcggatg atacacctac ttgtgcacca tcaaaataag
    31801 tggagcttaa ccctgaacca ctgcgcacat gattcaaata gcctgcttcg attctgtatg
    31861 tctgggcgga aagaggaaga taaaaataca atatcccggt caaaataaat acgggtttta
    31921 aagacttttt catgtatatg caattgtaaa aagttttctg aggcaaagat aagtcttttt
    31981 attttaaaga gaaacaagcc ctaaagtgaa aactaatgaa gttatgcttt atcttgttaa
    32041 cgccattttc tgtattactt acggggatga ttgatttcca cctttttggt tttctccata
    32101 attttatttc tttgttcgac agcagctact acattttcag ccagttgtcg gaaagaaacc
    32161 gataaaatgc tgtcttcttc cgttgcaatc ggtttcccgg catcgcccga ttcccgaatg
    32221 ctttgtacta tcggaatttg tccgagcaac gggatgttga attcttcagc cagtcgtttt
    32281 cctccttctt ttccgaaaat atagtatttg ttttccggaa gttctgcagg cgtgaaccat
    32341 gccatatttt ctaccaagcc aagtaccgga acgttcactt tttcgttggt aaacatattg
    32401 atgccttttc tggcatcggc caaagctacc tcttgtggcg ttgttaccac aattgctccc
    32461 gtaatgccca ttgtttgtac cagtgtgaga tgaatatcgc cggttccggg cggcaaatcc
    32521 atgataaaat aatccagctc gccccaatgt gcgtcggtga tcagctgttt gagggcatta
    32581 cttgccattg aaccacgcca taccaaagca ttgtcgggat cgacaaaaaa gccgatggac
    32641 agaagtttaa tgccgtattt ttcgatagga acaatcagtt ctttcccgtc aacttcttcc
    32701 aaaaaaggcc gtgcatcttc caatccaaac attttgggaa cggaaggtcc gtgaatatcg
    32761 gcatcgagaa gtccaacttt gtatccgagc gaagcaagcg ctacggcaag atttgcagca
    32821 atggtagatt ttcccacacc tcccttgccc gaaaaaacgg caataatatt acttactccc
    32881 ggaagaatgg agttttcttt ggctgtcgga atttcttttt tgaaaactgc tgttatgttt
    32941 ccttttatgt ccacttcttt tcccacataa gtcaacacag cttgttcagc tgctttaaca
    33001 agcgatttta caaacggatc gttgggcttt tcaaaaatca gagaaaagct tactttgttt
    33061 ccttcaatac ggatattatc ctgcaccata cccaaagata caatatcctg acctttgccc
    33121 ggataacgta cgtgtgaaag ggtttccaga attaatttag gatatagatt cattatttta
    33181 atgttctaaa atttttaatt tttagttgtt tcatgttttg gccttcttcc aaaactttta
    33241 tattgatcgt gctcgatttc tacttcaggt tcgagaattt cgattctttt ttcgagtttg
    33301 aattcgatat attttatggg gattcctctt tcgatccact gttcttcata aaaggttttg
    33361 atgctgagta taggatgatc actggcaatt tcatataaat tgtcggttga aaattctata
    33421 gggaaatggt tcaatttcaa cagttccatc gtataggtaa acaaaaaatt gctgtcagtt
    33481 ttcaaatgaa tcaaagcatt ttcggcagtg aaacggctat acagtttcag aaatccggat
    33541 gaagtaagtc ttttggtgat ttttttcatt tgtggatcgg ggaaagtcaa ccatatttca
    33601 ctgatttcat tctctccaaa aaagtaatgg agcatttcaa tgcgtgtacg caaaaaagct
    33661 acatttggaa ttttcttttc aaacgaatat ttagctcccc tccacatacg tgctccctta
    33721 atatcgacgc caataaagtt tttttcagga aaaatttcag ccaagccgac ggtatattct
    33781 cctttaccac atcccaattc gagaacaatg ggatttgaat tgccaaaaaa tttctcgttc
    33841 catttcccct gcatttcgaa atttgaatcc tcgttcctca caattttggc aggaaactga
    33901 aaaacatgtg gaaaagtttc catttcggca aattttgcca gtttgttttt actcacggta
    33961 ccgaaatttc ttgttacttg actttttcga ttttaatacc cacatcgtta atgccggcaa
    34021 ggatggaatc tcgcatccct tgaataattt ggtatcgata aattccggca cgaggaaaac
    34081 taactttttg acgataaaga atcggcattt cataaacgga accaactccc gacccgagcc
    34141 attttccacg ctgatcggca agatagcatt caatggtgtc tttttgcaag gtgtccatca
    34201 aaggagtagc ttgacgaagg aaaaaccaaa gattctgata cggatattcg cctcgatgac
    34261 ggatattgac gtaaacatta taaagagagg ttgtatcagt aatgggtaca tcgaaagtca
    34321 gcaccgaatc tttgttccaa ccttttgtgg gcagtgaacg atactgcaaa taaacatcat
    34381 ttcggttgca ggaaactatc aaacaggtga tggacaaaag caaaacaaat ttacttgttg
    34441 ttgttatttt ctttgttttg ttcatttttc ttgattggtt ttgcaatttt atgttcggag
    34501 gctggttgtc cgtttgtgtc gggattattt ttctgttttt tgtttttatt tttgaagcgt
    34561 ggatttttcc gttttcgctc gtcaaaacgg gtaacacttt cctgacctac aacattttca
    34621 aaatcctgta ctactcgagg ttgcgatgtt tcctccgaaa tttcctcgag tttttccggt
    34681 ttctgacctt ttttgttcat ggcaatcacc tctttagctc tttgaactga aatagttata
    34741 atgttggcac caaaattttg agcagtggaa taggttattt gtcccttgaa aacatctgtt
    34801 ttgaaatgat aataagtatt gtcaagcgtt tcgagctgaa tttctttgga tggaaattct
    34861 ttcatggctt cctggtaggt atccagttcg taattcatac agcactttaa tttgctgcat
    34921 tggccggcca atttttgtgg attgagcgaa atatcctggc agcgggctga gctggtggca
    34981 acagaattga aacaggtcat ccacgaagcg cagcacaatt ctcttccaca agggccaatt
    35041 cctcctatac gccctgcttc ctgacgggca ccaatctgtt tcatttcaat gcgcacatgg
    35101 aaagtttctg caaatacctt tatcagttcc ctgaaatcta cccgttcgtc ggcgatgtag
    35161 taaaaaatgg ccttatttcc atcgccctga aattctacat ctccaatttt cattttcagg
    35221 ttcatccttt cggctatttg tctggcctga atcatggtag cgtgttccct gtttttggct
    35281 tcctgatatt tttgaaggtc ggtatctttg gcttttcggt aaattcgcct gatttccatc
    35341 ttttcgggat gaacattgtt ctttttcatt tgcagcaata ccagcttccc taccagcgta
    35401 acttcgccaa tgtcgtgtcc cggtgatgat tcgactgcca ctatgtcccc tttctcaaga
    35461 ggaattttgg tactgttgag atagtatcct tttctggtgt ttttaaactg cacttccacc
    35521 aaatcggttt ctttctgcga ctccggcaaa tcggccatcc agtcgtgaac gctaagtttc
    35581 agattgccgc atcggttaca gcttttcttt tttatttggt aaccgcctat atctaatcga
    35641 taatccataa tttttgaatg ataatatttt attttccgga agacccaaat gaaaaataga
    35701 ttttattgtg ttcgattatt tctttaaagg ttttccgtct gttcataaat tttatttttt
    35761 aagcaattta gtgattttca acgccaaatc aaaaaaaacc atttttgaat tgacgttttg
    35821 ttcgatgtgc cgttctgcta acgcaaactc attcatcatg tcttcggcat tggtttcgtt
    35881 gataaatgga gcaaatccga ctgaaaattc tttttcaaac gaagtcataa aattcagttc
    35941 gggctgatgc agattgaaaa tgaaattttc gcgaatcatg cgttgtgcat aactcaaaaa
    36001 ttttttctgt ccttctctgc ccactgatgc ggcagccatt tcttcggacc attgccggat
    36061 ttttttcaat gatgcataat cgcctttgtt gccaactatc catgctaaac gcataatatt
    36121 tacaaaatgt tcaaaatttt ccctgttcac gttgttctcg tctatcagag ccaaagcttt
    36181 cagaaaactt ccttccgaaa tttgagctgc attggccgct tcattttcag aaatctgata
    36241 acggctaaca agtgcttcag caatttcctc attgcttaag cggggaattt taacctgttg
    36301 ggtgcgagat aaaatggtgg caataatttc ttcgtgagaa ttggaaacta aaagaaaaac
    36361 ggtgttttcg ggcggttctt ccagtatctt cagaagctta ttggcacatt ggtcgttcat
    36421 cctctcgggc aaccagataa gtataacttt gtactttgat tcataagtca tcaggttaac
    36481 ttttcgaata atttcgtctc cctcgctggt ataaatcatt ccttgcttgg cttcgccgta
    36541 aattttctgg taccagtcgt tttggctgaa ataaatacgt tctgcaaata tttgcctgaa
    36601 actgtccata aaatcatcac aaaccagtaa tttttttttc tccgatttga tgatagggaa
    36661 gacaaaatgc aagtcgggat gcgccagttt accgaattga acgcaggacg gacaaactcc
    36721 acacgcatcg ttttcgagcg gatgttcgca acagatgtat tgagcatagg ctattgccaa
    36781 cggaagtttt ccaatccctt caggtccata gaatagttgg gcatgaggaa ttttctgttc
    36841 cttcaccgtg cgaatcaatc gcttttttac ttcttcttgt ccgataactt gagaaaacag
    36901 catgacttgt cggaaaaatt attctctata ttcatgcaaa tttacgagat aattttgaaa
    36961 ttttaaatcc agcaaagaaa ataaagactt tattcgtaga gaatgaaatt caggttcctt
    37021 gagacagatg aaaatgtttt aatcgtaaga attcctcgat gcttgcggcg aggatagtca
    37081 attttaagga atttctttat ttcatgtagt ttgggtggat aaaagtatct tgctcatatt
    37141 atttcgctca gaaaaaattt gtttttgcaa aattttaaaa catcgcaaac cactaaaatg
    37201 ttgtaaattt gcatgaataa aatctttttc aataaatttt taatggacaa gtttttttga
    37261 tagtaaaaga aggcagatta tcatgaaaaa ttgaattttt tcgacaacaa acaaaatctc
    37321 aaattttctc aaactaaata aggttgttat gttttttgtc gaaaataaaa caaatcaaaa
    37381 aacgttaaat aaaatatcta attatcaatt gtttgcaaaa aaccataaaa tatggcacca
    37441 aaaaaaccag tgaaaaacgg cggacgaacg acttccggca aagttcagaa gaaatcggag
    37501 aaaaaatctt ccggcagcgg ttttggtaag aaaattgcaa gtttttttac cgacgaacgt
    37561 acacatttca tagtcggatt catgcttttg ctggtaatgt tgtacctgat gttggctttt
    37621 gtatcctatt tttttacggg cgcagccgac caaagcaaaa tcgatttgtc gtggagcgag
    37681 ttgcatgata agcgaacttt aattcagaac tggacttccg ttaccggagc cattgctgcc
    37741 gagcatctca tcaatcgttg ttttggcgta tcagctttct ttattccgat tttcggcatt
    37801 tgggtggctt tacgcttaat gaaagcgcat gaaaaatctg tttggaaggc attttttcac
    37861 tgtgcatttt ggcttgtgtg gctttcggta acgctcggat tgacactttc gccttatttt
    37921 tccgatgttt tgtttttttc gttggcgggc ggacatggcg attatgcagc acagtggctg
    37981 gtttcctata ttggtgcatc gggttcgttt ctgcttatgg ggggaagttt tatcgtttat
    38041 ctggtcatag cttctgcctc gtcgcttcct ttctttcgaa aacttttcgg aaagaaaaat
    38101 gtatcccata catcttccga agaaactctc gtagagccgg cagtggcagt gggtaccgaa
    38161 attgttcccg aagaatggat ttcatcaggc atagagactg aagtgtacga agaattctca
    38221 aaaaatcagg aagaatcaca aaccgaaaaa gtggcttttg aggtggaaac gaccgaaaac
    38281 aacgaagaaa gcaagccggt agatttggat ggcaacggag aaaatgaaag tttgcaggaa
    38341 gatccctatt acaaccttgc caaattggga aaatatgacc ctacactcga tttgtcgcac
    38401 tatcatttcc ctacgctcga tttgctcaaa gtttatggta ccgacaacga aacgcccatc
    38461 gacatggcgg aacagattgc caacaaaaac cgtattatca acactttgct caatttcgat
    38521 atcgaaatag aaaccatcaa agcaacggtc gggccaacca ttacactcta cgaaattgta
    38581 ccgaaagcag gggtgcgcat ttccaaaatc cgaaatctcg aatcggacat tatgctcagc
    38641 ctggccgcta ccggtattcg tattattgct cccatacccg gcaagggaac cattggaata
    38701 gaagttccca acaatgatcc gcaaattgtt tcgatgcatt ccatcattgc ttctaaaaaa
    38761 tttcaggaat cgaaatttga acttcccatt gctttcggtc gtaccattac caacgaaatt
    38821 ttcatggtcg atttggtaaa aatgccacac ctgctcgtag ccggagcaac aggacaggga
    38881 aaatcggtag gattgaatgc cattatcaca tcgttgcttt acaaaaaaca tccttccgaa
    38941 ctgaaactgg tgctggtgga tcccaaaaaa gtggaattca atatttatgc cggcatcgaa
    39001 aaacattttc tggcaaaact gcccgatggc agcgaagcca tcataaccga tacaagcaaa
    39061 gtggtcgaaa cgctgaattc tctttgcgga gaaatggata tgcgttacga tttgctgaaa
    39121 aaggcgcatg ccagaaacat aaaggaatat aacgaaaagt tcattttgcg tcatttgaat
    39181 ccggagaaag gacatcgttt cctgccctac attgtggtga tcattgacga atttggcgat
    39241 ttgatcatga cggcagggaa agaagttgaa atgcccattg cgcgaattgc acagctggct
    39301 cgtgctgtcg gcattcatat gattattgcc acacagcgtc cttcggtcaa tatcattacc
    39361 ggaattatca aagccaattt cccggcgcgg gttgcttttc gcgtttcgtc gatgatggat
    39421 tcgcgaacca ttcttgatgc tcccggtgcc aatcagctgg ttggacgtgg cgacatgctg
    39481 ttttcgcaag gaaacgacct gatacgagtt caatgtgctt ttgtcgatac tcccgaagtg
    39541 gaaaatatcg ttcattttat cagcgaacag cagagttatc ctactgcttt ctatttgccc
    39601 gaatatgtga aggctgaagg agaaatgggc gtagcgtctg ccgacttgag caaacgtgat
    39661 ccacttttcg aggaagcggc tcgacttatc gttgctaatc agcagggctc tacttcgttg
    39721 attcagcgaa aattttccat tggctacaat cgtgccggaa gaattgtcga ccagctcgaa
    39781 attgccggca ttatcggccc aaacgaaggg agcaaggctc gtcaggtact tattcccgac
    39841 gaatatcatt tggaacagat tttgaaacaa ctttgatggc tcaacaaaaa agcttgagat
    39901 aatcttttta attatcaaca aatttaaaat tttaactttt tccggtcgat atttccggat
    39961 ttaatcaact taaatcacca ctaaaaaatt ctgattatgc agttttcatt tcataaccca
    40021 agtcgttggg taaaatcatt catttattct atgctgttat cgataatttc gatacaaccc
    40081 gctctttcac agaatgatcc tgaagctcaa agaattatag acagtttttt gaataccgca
    40141 cgcaccggtg ccattactac gcgctttacc gtttcggtaa tcgaaaaaaa cggcgtaaat
    40201 tcgtacacga attccggaac atttacctta aagggtaacc gtttcgtatt ggagatgcag
    40261 gaaatgaaag tgtggtttga cggaaagaca caatgggctt accagaaatc aggcaatgaa
    40321 gttaccatta ccgagccgtc agagcaggag ttggctcaaa ccaatccgat ggcaattctt
    40381 ggtaattatc ggaacaagtg caatatccgt ttctgcaaga cttcttcgga gtattggtgc
    40441 attcagctga cacccaaatc gataaagaat gaagatattg cggaaattga agtacaattt
    40501 ctcaagacag acggaatacc gcaatccata acagtatctc aaaaaaacgg aacaagaaca
    40561 gttctgatgc ttagtcagtt tcagaaaaat gccaaagttt cggaacagat attcacgttt
    40621 gatgcaaaaa aatacaaaga cgcctatatc aacgatttga gataaaaatt tttaaaaaac
    40681 tacaaaaaga aatgacaact aacgaaaaga tacgatgcct gatcatagga tcgggaccgg
    40741 caggttacac tgctgccatt tatgcttcaa gggccaatct ttcgcccgta ctttatcagg
    40801 gtatggaacc gggaggtcag ttgacaacaa ccaacgaagt ggaaaatttt ccgggtttcc
    40861 ccgaaggaat taccggacct gagttgatgg acaaaatgca gaaacaggct gaacgttttg
    40921 gtgcggatat tcgcttcgga atggttacgt caaccgattt gtcagcttct ccctatcgcg
    40981 ttaccatcga taacgaaaaa attattgaag cccaaagcat tattattgca accggtgcta
    41041 cggcaaaata tcttggattg cctgacgagc agaaatatgc cggttcgggc gtatcggcct
    41101 gtgccacgtg cgacggattt ttttatagaa atcggccggt ggcagtagtg ggaggaggag
    41161 atacagcttg tgaggaagct ctttatttag ccaatattgc ttccaaagtc tatatgattg
    41221 tgcgcaagcc atttctcaga gcttcgaaaa taatgcagga acgcgttttg caacatccga
    41281 aaatcgaagt cctttttgag cacgaaacac tcggattaac aggcgaaaaa gtggtggaag
    41341 gggctgattt ggtgaaacgt cgcggacaac ccgacgaaga aaaagtgcac atcgccattg
    41401 acggtttttt tctggctatc gggcacaaac ccaattcgga agtatttaaa ccctggattg
    41461 aaaccgacga aaacggatat atcaaaacta ttcccggtac accacgaacc aatgtagaag
    41521 gagtttttgc agctggtgat gttgctgatc ctcattaccg acaagccgtg tcggctgccg
    41581 gttcaggctg tcaggcagct atcgaagctg aaagatattt ggtggataaa aaataaaagt
    41641 tagtaaagca gttttaattt tgctgtcgga aattaagttg aatgagggag ttaatactgt
    41701 ttttccctgc acaaatttat atgctgtgaa gttgtttttc tgtcaatgaa aaataattct
    41761 taacactatt ttcaaataaa atgaaaccca catgtattac atacggttgc ggtatatgaa
    41821 aagttgggca tttcggagca attaactgtc aaaccgcgca aagttaaatg cgagttaaac
    41881 acctcaaatt tagcactatc tgcccaattt tttatatacc gtgttatgca ctggcggttc
    41941 ttgtctgttt gtatgttaga gtcaaattgc actttcatga gaatataatt tattttttaa
    42001 aggtctgatg gaacccacat gtctaaatat ttatattcat tcgtgtttgt gtatgcaata
    42061 catgtgggtt tcatgttgtt gcttttgctt atgagtcaat ggcaaaaacc atgttgaata
    42121 cttactttga aatgtatcca caattatttc tttttatttt tataattaat ctttttctcc
    42181 gcagcaattt ttaccgattt ttcctcaatt gaatattcct ttgttttctt gcccttgccc
    42241 tgcgcttgcc gtatgagttt cttttctgct gccaatttct ctttctcggc ttttattctt
    42301 tcgagcaata cacttgccgg ctcgtcgttt gggtcttgtg ccaccagctc gccacgaaat
    42361 gctttggcta aaatactttg gggtagttta tccaacatgg ctttggcttt ggtgtaacgt
    42421 gcttctattt tatcggcaaa ggcaaagagt tgttcgac
//
LOCUS       DAOSIB010000008        38403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_1234_length_38403_cov_14.126213, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000008 DAOSIB010000000
VERSION     DAOSIB010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 38403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38403
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1234_length_38403_cov_14.126213"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            467..1690
                     /locus_tag="PLD44_01555"
     CDS             467..1690
                     /locus_tag="PLD44_01555"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOF98055.1"
                     /translation="MNKEEIEHIRADFPILSEKVYNKDLIYFDNAATTQKPRCVVEKI
                     EYAYFHLNANIHRGVHYLSLKATEAHEAARLTAAEFLHAEKKEEIIFTRGTTESINLV
                     AFSFGEAFCHPGDEVIVSAMEHHSNIVPWQMLCERKGMKLRVIPVNENGELDMVAYSS
                     LLNEKTRIVSVAHVSNVLGTINPVKEIIRIAHEKNIPVLIDGAQAVPHLSVDVRELDA
                     DFYVFSGHKIYGPTGIGILYGKEKWLNAIPPYQGGGEMISTVTFEKTTYNELPFKFEA
                     GTPDYVGSTALAEALRYINNIGLDNIAAYENELLHYAEQQLMEIENMHIIGTAKNKCS
                     VLSFLVDGIHPYDIGMLLDKLGIAVRTGHHCAQPIMDMFGIPGTVRASFAFYNTKEEI
                     VRFTEALKKVVNMLK"
     gene            1758..2180
                     /locus_tag="PLD44_01560"
     CDS             1758..2180
                     /locus_tag="PLD44_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOF98056.1"
                     /translation="MTINELQDEIIEQFSQFDDWMDKYALLIDMGNSLEPLENEYKTP
                     QNLIEGCQSRVWLDAQMKDGKIYFRGDSDAVLVKGIVYLLIHVLSGHTPDEILNADLY
                     CIDKIGLREHLSPTRSNGLLSMLKQMKMYALAFKSKQE"
     gene            complement(2187..2933)
                     /locus_tag="PLD44_01565"
     CDS             complement(2187..2933)
                     /locus_tag="PLD44_01565"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOF98057.1"
                     /translation="MIYFLSDAHLGSRLVENPREHEQKLVRWLEQVRRDAEAIYLLGD
                     IFDFWFEYKTVVPKGFVRFLGKLAELSDQGIEIHFFTGNHDIWTFGYLEEEIGMKVHR
                     EPLTVKLGNKLFYLAHGDGLYTNEPKFLFIRKIFHNSFAQRLFQLVPPRLGQKFGYWW
                     SKKSREKVLSLDNKYLGEENENLVIFAKNYILNHEVDFLVFGHRHIALDLQLQQQKRV
                     IILGDFVGIFSYGVFDGENFRLEYFEPQGE"
     gene            complement(3011..3325)
                     /locus_tag="PLD44_01570"
     CDS             complement(3011..3325)
                     /locus_tag="PLD44_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HOF98058.1"
                     /translation="MSDFLETEEKIVQMLRTVYDPEIPVNIYDLGLVYNIDVDEDGHV
                     EIDMTLTAPNCPVADFIIEDVRMKVESIEGIKDVDVNIVYEPAWDKSMMSEEAKLELG
                     LL"
     gene            complement(3420..3839)
                     /locus_tag="PLD44_01575"
     CDS             complement(3420..3839)
                     /locus_tag="PLD44_01575"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOF98059.1"
                     /translation="MTYTYELEMKVRDYECDLQGIVNNAVYQNYFEHTRHEFLQANHL
                     SFSELHQQGIDAVVARIEIAFKKPLLPGEVFLSKLYLRKEGVKYSFYQALFKKAEGTL
                     CAKAKVDTVVVINGKLASAFEPFEKLLADNHAVKGEE"
     gene            complement(3845..4471)
                     /locus_tag="PLD44_01580"
     CDS             complement(3845..4471)
                     /locus_tag="PLD44_01580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOF98060.1"
                     /translation="MSLEDYILQHSDSEPAYLTKISRDTHVKLLNPRMVSGHLQGRVL
                     SMLCKMIQPHKALEIGTFTGYSALCIAEALTDEDVLHTIEIDDELEDLILENFAGSEH
                     GHRIKLHIGNALEIIPTLNEVFDLVFIDADKREYTAYFEAVFPKLRNGGFILADNTLW
                     NGKVVTEAKPNDRQTMEILRFNDLIASDDRIEKVILPLRDGLTIIRKK"
     gene            complement(4686..7181)
                     /locus_tag="PLD44_01585"
     CDS             complement(4686..7181)
                     /locus_tag="PLD44_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOF98061.1"
                     /translation="MKIIHYLLLTCILASSCSRQVSLREVIDFTQDWKFTLSDSAMDA
                     SSPAFDDGAWRTLNLPHDWSIESDFSEKFPATAGGGALPGGIGWYRKTFFVPQSEQNK
                     CVYVDFDGVYRNSEVWINGHYLGKRPYGYISFRYELTPFLKFGEKNTLAVRVDNSKQP
                     NSRWYSGSGIYRNVWLTITQPIHVDLWGTYFTTPVVNENAATIQIQTSVRNSTGERRL
                     VKVEQMLLDNEGRKITKNETTIEVLPDSLAVASQSFSVNKPILWDIEHPYLYKMITKL
                     SVEGKETDEYETSVGFRYFNFDAQKGFSLNGKHLKINGVCNHHDLGALGAAINKRALE
                     RQLQLLKDMGCNAIRCSHNPPAPELLQLCDSMGFIVMDEIFDMWFKKKSAYDYAQDFP
                     KWYKTDMTDWIKRDRNHPSVFMWSIGNEVMEQWEDNITTDNMNLDQANLLLNNLKISD
                     SPKAKISESSLLTKHLSDIVKLLDPTRPVTAACNGTQKNNPLFLSGGLDIIGFNYHHN
                     EFMDVPKNFPGKPFIVTESVSAVQTRGFYLMPSDSIYIWPESWDKPFFNPLQKCSAYD
                     NSHVPWGSTHEKTWKIVKKHDFISGQFIWTGFDYLGEPTPYSWPSRSSYFGIIDLAGF
                     PKDIYYMYQSEWTDKDVLHVFPHWNWQKGQTIDVWAYYNNADEVELYLNGRSLGKRDK
                     KGDDLHVMWRVKYEPGTLKAISRKDGKEVLAYEVKTAGAPVKIRLTADRSTISSNSKD
                     LSFVTVELLDKDNNVHPLADQLVKFTLKGPGMIAGTDNGNENDSISLKKPQRHLFFGK
                     CMAIVQSTEKKGKITLKATVDGLPDEQIVITSK"
     assembly_gap    7528..7627
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7779..8387
                     /locus_tag="PLD44_01590"
     CDS             7779..8387
                     /locus_tag="PLD44_01590"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOF98062.1"
                     /translation="MLIKIYETNPNPHEIHRVIETLRDGGTIIFPTDTVYAFGCSLFN
                     KHGIEYIARIKKKDLRSSQLSFVCESIKQISEYTKLDDETFKLIKKNLPGPFTFILKG
                     NHRLPKLFKDKKTVGIRMPKNNIALTIVHELGNPLMTSSIFYHNNSLEYIVDPEMIEE
                     KYGHLVDLVIDGGPGGYIPSTIVDCTCEEPVILRQGAGELIW"
     gene            9172..9711
                     /gene="hpt"
                     /locus_tag="PLD44_01595"
     CDS             9172..9711
                     /gene="hpt"
                     /locus_tag="PLD44_01595"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOF98063.1"
                     /translation="MEIVQIKDKKFKKFIPEQEILSSIRKIGQEINKTLKDKNPLFIS
                     VLNGAFMFTSDLMKVVNIPCEITFIKLSSYEGLYSSGSVKEVMGLNENIVGRNVVILE
                     DIIDTGLTMEQIIDSLKAKGAGEIRVATFLQKPDALQRNIKIDYIAMKIPNEFIIGYG
                     LDYNGYGRNLKDVYTLITD"
     gene            9732..10313
                     /locus_tag="PLD44_01600"
     CDS             9732..10313
                     /locus_tag="PLD44_01600"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOF98064.1"
                     /translation="MLNIVICGAPGSGKGTQSKLIAEKYNLKHLSTGDMLRNEIKLQT
                     EIGKKAEEFISRGQLVPDEMIIAMLDHVLDKEMYHYKGIILDGFPRTYAQAEALEKLL
                     KKRNEEISLLLDLKVNDEELISRLLKRAEISNRSDDKLDVIRKRLEIYYQNADEVNNF
                     YKKTNKYVAIEGIGTVEEIFSRISQIIDSKINS"
     gene            10349..11500
                     /gene="obgE"
                     /locus_tag="PLD44_01605"
     CDS             10349..11500
                     /gene="obgE"
                     /locus_tag="PLD44_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOF98065.1"
                     /translation="MSGSNFVDYVKIFCRSGKGGAGSTHFHREKFAPKGGPDGGDGGR
                     GGHVILRGNKNYWTLLHLKYARHVFAEDGENGSKNRSFGKDGNDKIIEVPCGTVAYDG
                     ETGEFICDIQEDGQEFVLLQGGRGGLGNWHFRSATQQTPRFSQPGEPRKERTVILQLK
                     ILADVGLVGFPNAGKSTLLSVVSAAKPEIADYPFTTLVPQLGIVAYRDNKSFVMADIP
                     GIIEGAAEGKGLGLRFLRHIERNSILLFMIPAEEVDIREKYNILLNELKKYNPSLLEK
                     SKILAISKSDLLDAELKEEMRKNLPADVPTVFISSLTGEGILELKDLIWDELNKPSNQ
                     IIEISHAPIPISNQENEEELEEDFFPDNISSDMEETDRFEKMDWEDWDE"
     gene            11695..13419
                     /gene="recJ"
                     /locus_tag="PLD44_01610"
     CDS             11695..13419
                     /gene="recJ"
                     /locus_tag="PLD44_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOF98066.1"
                     /translation="MTNKWIFKSLSEEEKIIQKNLVESLSINPTLAQLLVQRGIQTFE
                     EARSFFRPDLAELHDPFLMMDMDKAIERLNKAIDNKEKILIYGDYDVDGTTSVALVYQ
                     FLKKYYSDIDFYIPDRYTEGYGISFQGIQFAAEQNFSLIIALDCGIKATEKVKHALNL
                     GIDFIICDHHTPDKELPPAVAILDPKREDCPYPYKHLSGCGVGFKLLQAFTMKKGIDF
                     SELTPLLDLVGLSIASDIVPITGENRILAYYGLKRLNSLPSLGIRSIINVCGLADREI
                     TISDIVFKIGPRINASGRMKSAREAVELLIANDAEIARQKSGNINEYNNDRKDIDKNI
                     TDEAISIINSQDVYKKRKSIVVYQPHWHKGVIGIVASRLSDEYYKPTVVLTRSNGLIS
                     GSARSVPGFDLYKAIDSCRDLLENFGGHMYAAGLSMKEENIQAFTERFEQFVSENILE
                     EQTYPQLEIDAVLEFKDITPKFFRILKQFAPFGPENMKPLFVSKKIFDYGTSRLVGKE
                     QEHLRMELIDSSSENVMNGIAFGMHEYNDHLKALHPLDICYTLEENNFNGNSTIQLMI
                     RDIKTEEY"
     gene            complement(13457..14629)
                     /locus_tag="PLD44_01615"
     CDS             complement(13457..14629)
                     /locus_tag="PLD44_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOF98067.1"
                     /translation="MMEQTERPYTFDRVVRLVITLTVLTALFFLTQRLSGVLLPFLIA
                     WLLAYLLQPIVHFFQYKLKFKYRFVAIFATLLLFIIILTGLGFLLIPMIINEIEKAVV
                     LINSFLRNVDVNSFLPIAWQNQLSEYFRQLDLQTLLGSEKFMSLVQKLLPEVWNFVGN
                     SLNFILGLAVIVIIFLYLFFILMDYEKISEKWIELVPMKYRPLITNIVQDIQDGMNRY
                     FRGQALIAMCVGILFMIGLSIIRLPLAIVIGLSIGILNLVPYLELLGIIPCAFMALLK
                     ASETNQNFWMIALSIAAVWLIVQSIQDLILVPRIMGKYTGLHPALILLSLSIWGSLLG
                     FAGMIIALPFTSLILSYYKRFIIKGENIEHPNVPQPEQTVSPVSSEECVSEEPKQD"
     gene            complement(14650..15240)
                     /locus_tag="PLD44_01620"
     CDS             complement(14650..15240)
                     /locus_tag="PLD44_01620"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006045252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOF98068.1"
                     /translation="MFYSEQTSHASRRGSIEVICGSMFSGKTEELIRRLKRAQFAKQR
                     VEIFKPVIDTRYSESEVVSHDRNAIRSTPVGSSGSILLLSANVDVVGIDEAQFFDDGL
                     VEVCNQLANQGIRVIVAGLDMDYQGVPFGPIPALCAIAEDVFKVHAICVRCGALAYVS
                     HRLVDSNKRVLIGEMQEYEPICRNCFNEIQRSQMKI"
     gene            15427..16320
                     /locus_tag="PLD44_01625"
     CDS             15427..16320
                     /locus_tag="PLD44_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98069.1"
                     /translation="MNKDLILALLHKNIQDLDMLTESFMEMNELPPAILHLAMQKVDE
                     IHQYLEELNENNSTKKPAGKEIFPSEDLKDKKENHFLHFSEHSTNDFAEPSENVSQQT
                     EIEPIQEMEFQVGEENIAQETEKTEFSFTESPINEKLTEWDETEKETAEIEQEPLENE
                     KNRFVSTQPEIGDETTEITDSEAQEKEEPSANEGQKTAPTSIPLQEKFSKIPDNSLGT
                     TFANQKIDDIKKAISIGDRFRFQRELFRGNGEDMNKTLNYLNQLATLEEAVSFLQAKY
                     KWDGENETVQDFFQILKRRFL"
     gene            16448..17191
                     /gene="rsmI"
                     /locus_tag="PLD44_01630"
     CDS             16448..17191
                     /gene="rsmI"
                     /locus_tag="PLD44_01630"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOF98070.1"
                     /translation="MSQQKNKTSLAGTTINNFSASKEGGKLYVVPTPIGNLEDMTFRA
                     VKILKKVNLILAEDTRTSSFLLKHFGIETPMQSHHKFNEHKTVENIVQQLKSGQILAL
                     ISDAGTPAISDPGFLLVRECVENGIEIECLPGATAFVPALVTSGLPNDRFCFEGFLPV
                     KKGRQQRLKELETEKRTMIFYESPFRLLKTLTQLSEVFGENRKASVSREISKMFEETV
                     RGTLAELIQHFTEKTPKGEFVLIVAGFEE"
     gene            17322..18215
                     /locus_tag="PLD44_01635"
     CDS             17322..18215
                     /locus_tag="PLD44_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98071.1"
                     /translation="MNKNISYRNIRVFSVIFLVAFLTVSCGKQSSDYKKLKAENDSLL
                     KEKERLEREVDDYFSSINQIQENLEKIKSTENVISVEPAGEELSQDARTRINEDINYL
                     NELLQTNKDELARLKSRLSKSSLKLTELERTITRLTKTLEEETARIAFLEKKLAEKDT
                     LIAQMGSTISQMGENINTLTTENKSKEEKIKQLDENLHSAWYVFGSRKELIDQKILTS
                     KGIFRKAEVLKDSFNKDYFVRIDARNAKTIPLYASKAKILTTHPTSSYTIEKQDGKLV
                     LIIVDPTAFWSVSKYLVIEVD"
     gene            18339..19046
                     /locus_tag="PLD44_01640"
     CDS             18339..19046
                     /locus_tag="PLD44_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HOF98072.1"
                     /translation="MNNKHPYRYIFLDLDDTIWDFHANARLSLQKMYEQQTLNRYFEN
                     FDEFFRIYAMRNMELWEMYGKGEITKDFLMIERFRYPLAKMGLDDLQLAEAIGKQYLE
                     ILPEQTTLLPFARELLDYLHSKYPLTLISNGFVEVQYKKLKSCQIEHYFSHVVLSEQA
                     GALKPDPEIFNYALHLNQASPEEAIMIGDSFSADIVGAKNAGIDQIYLQKNNMLPYTK
                     QQATFIVKSLEEIFSIL"
     gene            19167..19499
                     /locus_tag="PLD44_01645"
     CDS             19167..19499
                     /locus_tag="PLD44_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98073.1"
                     /translation="MNTNEDNLLEYSDEEAVKFILKSLPAEVKDRIDEDTVQYVLDVI
                     YDFYNEKGYIDEDDTAEETSIDEEEMYEYILENALKDEINLTEDDIQMILDAEFDYGK
                     SLGIYREE"
     gene            19611..20492
                     /locus_tag="PLD44_01650"
     CDS             19611..20492
                     /locus_tag="PLD44_01650"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOF98074.1"
                     /translation="MIYPLLWLLSLLPLKVLYAFSPLIYFLIYRVAGYRKTVVRQNLQ
                     NSFPELSASERRKIEKNFYRFFADLLLEIVKLLHISDKELLQRVKFVNVEEFFECCRQ
                     GKYPILMLGHYGNWEWILTISLLMPENCESFTVYKRLKNRKFDQFMKSLREKRGAVMV
                     EKRNLARNMIRWAKEGKFGAYGFIADQSPSGKNLRNWTTFLHQPTAILPGAEQLARKF
                     NYPVFYARVSRPKRGYYSCEILPIAREPRTTSDNEISTRFMQLLEEDILAHPELWLWS
                     HRRWKIKPLIDEVKKDN"
     gene            20645..21679
                     /locus_tag="PLD44_01655"
     CDS             20645..21679
                     /locus_tag="PLD44_01655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOF98075.1"
                     /translation="MQVSVVILNWNGKNYLQQFLPILLEKTRLSYVEIVVADNGSTDG
                     SLQLLKEQFPMVRIILFDKNYGFAEGYNKALSQIKSDYYVLLNSDVEVTDNWLLPLVD
                     YMEKHPQTAACQPKILSYFNRTHFEYAGAAGGFIDYLGFAFCRGRIFGEVEEDKGQYD
                     DPMNVFWTSGACMMVRAGLYHKMGGFDADFFAHMEEIDLCWRFNSRGYQLTCVPESMV
                     YHIGGGTLQSEHPFKTYLNFRNNLLMLYKNLPKKQLKSVFFKRAILDYLAVFQFLASA
                     KPKNARSILKARSDFRKMKPNFLDKRKENILFSTATSFPMIYPRSIVVDYYLRKKKKF
                     TTLKFDQKPN"
     gene            complement(21688..24111)
                     /locus_tag="PLD44_01660"
     CDS             complement(21688..24111)
                     /locus_tag="PLD44_01660"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOF98076.1"
                     /translation="MRKIKICRISEKSLLLFRFMMGILLTEVLFVAGLQAQETIVVGQ
                     VIDKYDKHPLSSVSIYFKGTSVGTTSNEEGYFLLRHTGKENILVFSAIGYKKTEIKIK
                     PGSNIGLTVEMEESNTLLQEVFILPGANPALDLMRKVRLLRKVNDETKLPHYEVLATE
                     QNAIFLSRSQHRSISKQIFRQLQEGNLLSSDSSLLVPLYMDEIKYQLTSTAKTELSKK
                     IFSSSEEGNKILEQLFGELNPSLNFYENSIPVFNKNIISPLASIGNMYYNYYLADSMV
                     TSSGKQYEIHFRSRNPRNPAFNGKFCLDSASLALTYIEAELPSEANINFIRHLSIKQE
                     FVPFSSKRWVRNKDQLSLVLNLDILTDSVPAGSQIFFKRSTIYNTGSIQIPESFAQSG
                     YSEETLNQKLQQLGKTPLMNAAKWLADIIFTGYIPVGKIDIGKIQYLARTTDVEGLRL
                     TLPFRTNEKMWKNFTVGGFVGYGFKNEAIKYSGFTQYKLPAEKRRIIGLNYTNDYRRI
                     DYNYNNFLYLENPLVSGDEDIAGTIFALRSAGRLNERKEWTLSFSGDWNRNIESGLYF
                     RSLQQLPSDALPLKKDGVDINNFQQQSFTLSTRFSFNEKKYEDHLQRIYINNRNPIIY
                     GILEVGKYELGNTEKYYGKVSGILKQNVQFDLGQVNYWMEAGSIFGSVPYPLLAYQPG
                     AEGGYGFYRFSMMNFMQFAADKYFDFHGELIMNGLILNHIPLLKYLNMREMFSVKFGY
                     GKMSNDHLSVMDFPAYVYPFNRPYTEVGVGLANILHLFNLQAIWRLTDIKQFSGDNFA
                     IRGSLRISF"
     gene            complement(24080..25003)
                     /gene="xerD"
                     /locus_tag="PLD44_01665"
     CDS             complement(24080..25003)
                     /gene="xerD"
                     /locus_tag="PLD44_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOF98077.1"
                     /translation="MNSLLTDYHYYLKFEKALSENTIQAYERDLEKLNSYLSDFHVLL
                     QDATIELLRDFIIEIGSLGIHPRSQARIISSISSFYRFLVYKNIISTDPSELLESPKI
                     GLRLPQILSVEEIDAMISAIDLSKPEGQRNKAIIEVLYGSGVRVSELINVKLSQLYLN
                     EGYMMVEGKGSKQRLVPLSPNSIKQIELWKIDRNHLEIKKGHEDFLFLNRRGEKLTRA
                     MIFKIVKDLATMAGIQKNISPHTFRHSFATHLLENGANLRAIQQLLGHESITTTELYT
                     HIDVQFLRETILKYHPLNQSKNEENKNLSDF"
     gene            25193..25978
                     /locus_tag="PLD44_01670"
     CDS             25193..25978
                     /locus_tag="PLD44_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HOF98078.1"
                     /translation="MLCKVKTLFVILFCAINFALSAYDVTGHRIIASIAYQNLNAATR
                     NRVDKLLGKQGIVYEAGWADEIRSDDNYAYSYQWHYQNLKDSLTSKDLKFLLEHPKVE
                     GKHLFFALDSLSGELKKNPNNAEALKFIIHFVGDLHQPMHLGRVDDLGGNKIMVKWFG
                     AKSNLHRVWDSQLLESSKYSYSEYSIYLQNKFEPQRATYQKFSLLQSAEAVYGLRNQI
                     YAYDLTTETNNYKYIYLFSDKLDEMLYLGGIQLANVLNGIYSK"
     gene            complement(25984..26760)
                     /locus_tag="PLD44_01675"
     CDS             complement(25984..26760)
                     /locus_tag="PLD44_01675"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOF98079.1"
                     /translation="MKKVLIIILLFGVQISFFVVHAQKNGLIGKSVIPNSKSIFFAAA
                     GPAYCFGDVGGSHYEQFLNGINDWDVLNTRFLFSLGYGQTFKHNIGYKLIFNYGNFTG
                     NDKNSRNEARQYSFNSEVSSLSLQAEYTFLGGIHSNYYDYYSLYLFGGIGVMNSNAEV
                     KKNGVAISQPPPDRIYDKIKLNETAAFIPFGIGYKYSITPKFSIGAELSWNYAFTDFI
                     DGISTVTSKENDLLANLSITFSYKFFDSLTPACRCLPYNQ"
     gene            complement(26853..28514)
                     /locus_tag="PLD44_01680"
     CDS             complement(26853..28514)
                     /locus_tag="PLD44_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOF98080.1"
                     /translation="MGKTVTIYCKNTRSYHDFPLGTSLIEIYRSLNIQLKYQVVAARV
                     NYKVEDLNFLIYKPKDIEFIDLSTSSGMRVYVRTLTMVVAKAVSELFPKGNIRIEHPL
                     SKGYYCVLNNIDEPLTQSLVDKIKERINLIISQGKTIICEEKQTSEVIKLFADQKDKI
                     VLFQTLGNPYCRYFRIDDYIDYYNGVLLPSTDYLNLYDLIPYYDGLLLRVPNRENPTE
                     LEEIVPQPKLYDIFKEYVEWNKIMNLSNVGEFNVACRNKQAFSLIKISEALHEKKVAT
                     IADTIAQRKDKVKFILISGPSSSGKTTFSKRLSVQLAVCRIKPVAISLDNYFVDRDKT
                     PRDENGELDFEHLHALDLELLNQQLRELIEGKEIELPSFNFEEGRRYYRGDKLKLAED
                     SLVILEGIHALNPKLLPEIPAEATFKIYVSALTTISIDNHNWIPTTDTRLLRRIIRDS
                     RYRNYSARETIARWESVRKGEEKWIFPFQENADVMFNSALLFEFAVLKRYAEPLLAEV
                     PKYCDEYTEAHRLIKFLNYFVPIPDREIPPTSLLREFVGGSSFRY"
     gene            28646..30445
                     /locus_tag="PLD44_01685"
     CDS             28646..30445
                     /locus_tag="PLD44_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOF98081.1"
                     /translation="MHYTFYDLLTLIGSLGLFLYGMKTMSEGLQKATGDTLRSIMAAM
                     TRNRFTGILTGLLITSLIQSSSATTVMVVSFVNAGLLSLYQAITVIMGANIGTTITAW
                     MISIFGFKVSIAAFSIPLIGISIPLIFSSKSRNKSIGEFLIGFALLFMGLELLKNSVP
                     DIQGNPEVLNFLAKYTNFGFGSILLFLLIGTMLTIIVQSSSATMAITLIICSKGWISY
                     ELGAAMVLGENIGTTITANLAAIPTNVSAKRAALSHLIFNIFGVIWMLIAFRPFINLV
                     TMLITQFGPGDPTQLTAFSNSLDPDTLKVISSGSENLSPEQIAVRNQLENYQIATSYG
                     LSLFHTLFNITNTLVLIWFAKIIEKISTLLIKKKDTDEEFQLQFISTGLLSTSELSLI
                     QAWKEIKVYAERSHRMFSLVRELLQEKNENDFIKKFSRVQKYENISDRMEVEIAHYLT
                     KVAEGKLSEKGKFQIQTMLKVISEIESIGDSCYNLARTIKRKKDDKAEYTKEMDDNLN
                     EMMNLVEDALLQMRAAIDNNHVSKEELNRAENLENEINNFRNQLKNKNIDDVKENKYD
                     YQASVTYMDMLEECEKLGDYIVNIVEALYEASK"
     gene            30667..31602
                     /locus_tag="PLD44_01690"
     CDS             30667..31602
                     /locus_tag="PLD44_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HOF98082.1"
                     /translation="MDYILIISGFAVLVLGANLLVDGASSLAKKLKISDLIIGLTVVA
                     FGTSAPELVVNVIASVKPNATDIAISNVIGSNIVNIFIILGFSALFHPIKSEKTTTLF
                     DIPLSFFAIVVTYLLAFTGDRILSRTDGLVMLGFFIAFIYFTTRRSIKHPLIPLEENI
                     KIRKTGISILMISCGLAALIGGAEIIVPSAIRIAENLGISQAVIGLTIVALGTSLPEL
                     ATSVVAAIKKNSDIALGNIIGSNIFNIFLILGVSSVIRPLPIYRGIGIDLTMGALGSV
                     MVFVFVFGSKSHSIKKWQGLLLIFIYLMYAIFKIF"
     gene            31643..32767
                     /locus_tag="PLD44_01695"
     CDS             31643..32767
                     /locus_tag="PLD44_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOF98083.1"
                     /translation="MNLFDVYPLFDIEIVKGQGCRIYDKNDTEYLDFYGGHAVISIGH
                     SHPYFVKKLADQIQKLVFYSNSVKNSLQVELAEKLGKIAGYDDYSLFLINSGAEANEN
                     ALKLASFHNGRSKVIAFGKSFHGRTSAAVGVTDNPAIQAPINRNFEVEFLPLNDIEAV
                     LQSLKNRDVCAVIIEGIQGVGGIQIPQPAFLEAISAACKETGTVLILDEIQSGYGRSG
                     KFFAHQYADIRPDIITTAKGMGNGVPIGGVIISPMFKPTYGMLGTTFGGNHLACTAAI
                     AVLDVIEQEHLMENALQVGNYLINELQKFSRLKEVRGKGLMIGIEFDEPVKKLRERLL
                     FEKKIFTGVSGSNIIRLLPPLCLTMQDAELFLDHFAEVIK"
     gene            32790..>33403
                     /locus_tag="PLD44_01700"
     CDS             32790..>33403
                     /locus_tag="PLD44_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98084.1"
                     /translation="MKKPIALLFILFFVNSWTVFSQSVSKERDKVVMKTGEIYYGEVV
                     FRNDEMLILQTDEGVRYQFSIAEVKAIYKETVDGSDELEKMQQEGQSDEIQNFFAVLD
                     ACGGISSAKNSFDVSSNTQVAFVLGKDKVFSENFFVGLGVGYNSSYLKSNSSLNFLPV
                     FLRLQNTFTNNRISPFVRMDAGYAFSMTSGWDGGALVKAAAGISS"
     assembly_gap    33404..33503
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <33504..34181
                     /locus_tag="PLD44_01705"
     CDS             <33504..34181
                     /locus_tag="PLD44_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOF98085.1"
                     /translation="ALNEQACAEKAQHHLRFFKTAPGEYGEGDKFIGVNVPDQRKIAK
                     KYFREINLNELEVLLTNEIHECRSTALFMLCYKFDKAGEAERTLMADLYLNNLSFVNN
                     WDLVDASAANILGCYLMDKKRDLLYDFVQSNDLWKQRIAIIATFYFIRCGEFVDTFKI
                     SEMLLHHPHDLIHKAVGWMLREVGNRDKEAEINFLLKHYRQMPRTMLRYAIEKFDEGL
                     RRQFLKG"
     gene            34411..36870
                     /locus_tag="PLD44_01710"
     CDS             34411..36870
                     /locus_tag="PLD44_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HOF98086.1"
                     /translation="MKLKEIAQILGNSHLKVPEVEINWLLTDSRALSFPTRSLFFALR
                     TRRNDGHRYIQELYDQHLRYFVVSETLPEFEKMHDAIFIRVSDTLEALQHLAAVHRNA
                     FQIPIVGITGSNGKTIVKEWLYQLLHSDFNITRSPRSYNSQIGVPLSVWGLNNNTELG
                     IFEAGISQPHEMERLEPIIRPTIGIFTNLGDAHQENFSSLKQKCEEKLKLFAHAQVLI
                     YCSDNRLVDITVAQSNFKGKLFSWGRNENAILQLVKTKKLSDSTEVVYKFSGKESAVS
                     IPFSDDASIENAMHCVALMLHLGIDSEKIRQRIAKLESVEMRLELKEGIHGCLLINDS
                     YNSDLNSLAIALDFLNQQAATKNLPRTLILSDILESGKQSDDLYNAVAQLVKSKSIQR
                     FVGIGPEIVKHADKFEVEQQNFFEFTEDFLKSPLIHELQKEIILLKGSRKFHFEKISE
                     KLELIAYETTLEVNLNALINNFNYFRSKLRPETKVMSMVKAFAYGSGSVEVARTLQLH
                     HCDYLAVAVADEGAELRREGIRIPIVVMNPEKGSFGMIFEHQLEPEIYSFRILESFLS
                     AAEKLGVTDYPVHIKIDSGMHRLGFEPHEIVPLIQRLKAQNNLKVRSVFSHLAGADEK
                     RFDDFTRQQVKVFTECADLFAQSFEHPIIRHILNSAGIERFPEFQFDMVRLGIGHYGV
                     SALPNVKLEPVCALKSIILQIKNVKKGETVGYSRKGIANEDKRIAVIPVGYADGFDRK
                     LGNGVGEVLINGKRAKVMGNVCMDLTMVDITGIEAKEGDSVEIFGNNLSVIEVADKLK
                     TIPYEVLTSVSKRVKRVYFQE"
     gene            36918..37688
                     /locus_tag="PLD44_01715"
     CDS             36918..37688
                     /locus_tag="PLD44_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOF98087.1"
                     /translation="MILIFVQNTTPPVALLQSVATLSVQLKKEWGILCLTENADLVSV
                     YENQYGEFLKTNGFPEVSIFVRQARCEQLAEFCEQLEASFLFFQLHDASHRYLRRALN
                     FCRDLRIPYLFFKNSFSVLNLEKVVVPIGFLEEEIEKAQFASAFGRFSNAEIILLPAN
                     DYGSKAATTTDKMKTILDKFQLPYTVEKAQSDSFKIDKEAIEKAVEMGAGLVIISASR
                     DYGLDDILFGPKELHLIRKSPVPLLLVNPRSDLYALCD"
     gene            complement(37705..38355)
                     /locus_tag="PLD44_01720"
     CDS             complement(37705..38355)
                     /locus_tag="PLD44_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOF98088.1"
                     /translation="MKSLHIGYIPDLDKADITTASVILEKNGTKAAIESQNWAKEFPY
                     KPIAYFHIAQSKEALFIKFFVLCNALRAVYSRDQEPVYEDSCVEFFCKTIESEKYINF
                     EFNCIGTCSASRRKGRNDQVEPLSETEMKSIERFPSIVPQPFEEIKGNFDWELTVKIP
                     FKLIGIDPNNLPEKILGNFYKCADGTESPHYLSWNPIKTEKPDFHRPEFFGELFLK"
BASE COUNT        11996 a         7205 c         7361 g        11641 t
ORIGIN      
        1 tttattaaga atcaaaatag agatcacaag taaaaaataa ctttgtgatt aaacttattt
       61 cgaattattt gcaacggatg ttaagcatag atttaattta cttcgccatt gattgagaaa
      121 atctttgttt ttgcgtaaca ttcgagtgtt tggtctgata aaatgagatt gtgccaaatg
      181 tttaatgatt ttcatcagaa tataatttat tttttaaagg tctgatggaa cccacatgtc
      241 tgaatattat attcattcgt gtttgtttat gcaatacatg tgggtttctt aacttttatt
      301 gacagaggaa ccctatttcg tttttaaagt ttatagatta ctttccttga aagtccaaat
      361 taaaactcat tgataaaaac ttctcacaaa atttacaaaa gagtgtttct tcattttttt
      421 gtaattttgc aaaaacaaaa acagacttcc gatagttgta taaaacatga ataaagaaga
      481 aattgaacat atcagggccg attttccgat tttaagcgag aaggtttata acaaggattt
      541 gatttatttc gacaatgctg ccaccacaca aaaaccccgt tgcgttgtag aaaaaatcga
      601 atatgcttat tttcacttga atgccaatat tcatcgtggt gttcactatc tgagtctgaa
      661 agccacagaa gcgcacgaag cagctcggct gaccgctgcc gaatttcttc atgcagaaaa
      721 aaaagaagaa attattttta cacgaggcac cacagaatcc atcaatctgg tagccttttc
      781 atttggtgaa gctttttgtc atcctggcga cgaagtgatc gtttcggcaa tggaacatca
      841 ttccaacatt gtcccttggc aaatgctttg cgagcgcaaa ggaatgaaac tgcgcgtaat
      901 tcctgtcaac gaaaacggag aactcgacat ggttgcctat tcttctctgt tgaacgaaaa
      961 aacgcgtatc gtatcggtgg ctcatgtttc caatgtattg ggaaccatta atccggtgaa
     1021 ggaaattatt cgtattgctc acgaaaaaaa cattcccgtt ttgattgatg gagcgcaggc
     1081 tgttccacat ctttcagttg acgtacggga attggatgcc gatttttatg ttttttcggg
     1141 acataaaatt tatggcccta ccggcatcgg cattctttac ggcaaggaaa aatggttgaa
     1201 tgccattccg ccttatcaag gtggcggaga aatgatttct accgttacct ttgaaaaaac
     1261 cacttacaat gaacttccat tcaagtttga agccggaaca cccgattatg tcggctcaac
     1321 agctttggcc gaagcgcttc gatatattaa caacatcgga ctcgacaaca ttgccgctta
     1381 tgaaaatgaa ctgctccatt atgccgaaca gcagctgatg gaaattgaaa atatgcacat
     1441 tatcggcaca gcaaaaaaca aatgttccgt tctttctttt ctggtcgatg gaatacatcc
     1501 ctacgacatt ggcatgttgc tcgacaagct gggaatagcc gttcgaaccg gtcatcactg
     1561 cgctcaaccc attatggata tgtttggaat tcccggcacc gtgcgtgctt catttgcttt
     1621 ttacaatacc aaagaagaaa tcgtacgctt tacggaagcc ctcaaaaaag tggtaaatat
     1681 gctgaaataa atgttgcaga aaaaaataca atttcatgaa aatttttcca aaaaaacatt
     1741 aaattatcag aaagaaaatg acaatcaacg aattacagga tgaaataata gaacagttca
     1801 gtcagtttga cgactggatg gataaatatg ctttgcttat cgatatggga aattcgctgg
     1861 aaccactcga aaatgaatat aaaacgcctc aaaatctaat tgaaggctgt caaagtaggg
     1921 tgtggctgga tgcacaaatg aaggatggaa aaatctattt tcgaggcgat agcgatgcgg
     1981 tacttgtaaa gggaattgtt tatctgctca tccatgtttt gtcgggacac acgccggacg
     2041 aaattttgaa tgccgatttg tattgcatcg ataaaatagg cttgcgcgaa catttgtcgc
     2101 caacacgttc caatggtttg ttgtccatgc tgaaacaaat gaaaatgtat gcgttggctt
     2161 tcaagtcgaa acaggaatga atcggattat tctccttgcg gttcaaaata ttcgagtcga
     2221 aaattttctc catcgaaaac gccataagaa aaaattccga caaaatcgcc aagaataatt
     2281 accctttttt gttgttgcaa ctgcaaatcg agtgcgatat gtcgatgtcc gaaaaccaaa
     2341 aaatccacct catgattcaa aatataattt ttggcaaaaa tcacaagatt ttcattttct
     2401 tctcctaaat atttattatc caggctgaga actttctccc tgcttttttt cgaccaccaa
     2461 taaccaaatt tctgccccag tcgcggcggc acgagctgaa acaatcgctg agcaaaggaa
     2521 ttgtgaaaaa tttttctgat aaaaagaaat ttaggttcat ttgtgtacaa accatctccg
     2581 tgagccagat aaaaaagttt gtttcccaat ttcaccgtca aaggctcacg atgcactttc
     2641 atcccaattt cctcctccaa atacccaaat gtccaaatat cgtgattgcc ggtaaaaaaa
     2701 tgaatttcaa ttccctgatc gctcaattcg gccagtttgc ccaaaaagcg aacaaaccct
     2761 tttggaacga ccgttttgta ttcaaaccaa aagtcaaaaa tatcacccaa caggtaaatg
     2821 gcttctgcat cccgtcgcac ctgctccaac caacgaacaa gcttctgttc gtgctcgcgg
     2881 ggattttcca ccaaacgtga acccaaatga gcatcggaaa gaaaataaat catactttct
     2941 tccctcctct cgctgcagta gttttacgat tgaaatttat atgtgaaata tttctcaaaa
     3001 tatatttttt ttacagtaat ccaagttcca gctttgcttc ttcgctcatc atgcttttgt
     3061 cccacgccgg ctcgtacaca atgtttacat ccacatcttt tatgccttca atactttcca
     3121 ctttcatacg gacatcctca ataataaaat cggcaacggg acaattgggt gccgtcaaag
     3181 tcatatcaat ttctacatgt ccgtcttcgt caacatcgat gttgtacacc agacccaagt
     3241 cataaatatt gacagggatc tccgggtcgt aaaccgttcg gagcatttga actatctttt
     3301 cttccgtttc caaaaaatca ctcatgttaa tggttgttgt ttgttccatc aataaaaaaa
     3361 tcctacagct tttcaaacac ttcgagaagt caaattgttg tcggcacaag aaaaattttt
     3421 tactcttcgc cttttacggc atggttgtcg gccaaaagtt tttcaaaagg ttcgaaagcc
     3481 gaagccaatt ttccgttgat cactactact gtatcaactt ttgctttggc gcaaagtgtt
     3541 ccctctgcct ttttaaataa agcctgataa aaactgtatt ttactccttc ctttctcaga
     3601 taaagtttgg aaagaaatac ttcgcccggc aataaaggtt ttttaaatgc aatttcgatg
     3661 cgggcaacta ctgcatcgat gccttgctga tgcaattccg aaaaactgag atgattcgcc
     3721 tgcaaaaatt cgtgccgcgt atgttcaaag taattttgat aaaccgcatt atttacaatt
     3781 ccctgcaagt cgcactcgta gtcgcgcacc ttcatttcca gttcataagt ataagtcatg
     3841 gcagttattt ttttctgatt atcgttaacc catctcttaa gggcaatata actttttcta
     3901 ttctatcatc ggatgcgatt aaatcgttaa accgcaaaat ttccatcgtt tgtctgtcgt
     3961 tgggttttgc ctccgtaacg acttttccat tccacagggt gttgtcggcc aaaataaaac
     4021 cgccatttct gagttttgga aaaaccgctt caaaataagc tgtgtattct cgtttgtcgg
     4081 catcaataaa taccaaatca aaaacttcat tcagagtcgg aataatttcc agtgcattcc
     4141 ctatatgcaa cttaattctg tgtccatgtt ccgaaccggc gaagttttcc aaaatcaaat
     4201 cttccagctc atcatcaatt tctatcgtat gcaaaacatc ctcatccgtc aatgcttctg
     4261 caatgcacaa agccgaatat ccggtaaaag tacctatttc aagcgcttta tggggttgaa
     4321 tcattttgca caacatggac aatactctgc cttgcaaatg tcccgaaacc attctgggat
     4381 tcagaagctt gacatgtgta tcacgactga tttttgtcaa ataggcaggt tcactgtccg
     4441 aatgttgcaa tatgtaatct tccagcgaca tggatgctta atctttaaaa aatgaaattc
     4501 ggttacaaat caaccaaatg tacccttctc ctttaagaaa tattttcttt ctgatggtgc
     4561 aaaattatcc attttttgtc atatttcatt gattcgaaat gtcaagctct gttttttata
     4621 aacaatgttc agggaaaaac taaaccctgt ttataaaatg actataaata gggttgagtt
     4681 tatctttatt tacttgtaat aacgatttgt tcatccggca aaccatctac cgttgctttt
     4741 agtgtaattt tccctttttt ttcggtgctt tgaacaatgg ccatacattt tccgaagaac
     4801 aaatgacgtt gaggtttttt taatgaaatt gagtcgttct cgttgccgtt atcggtgccg
     4861 gcaatcatgc ccggtccttt tagcgtaaat ttcaccaatt gatcagccag tggatgaaca
     4921 ttattatctt tgtcaagcag ctctactgta acaaatgaca aatctttact attggaagag
     4981 atagtgcttc tatcggctgt caggcggatt tttaccgggg ctccggctgt tttgacttca
     5041 tacgccagca cttcttttcc atccttgcgt gaaatagctt tcaacgttcc gggttcatat
     5101 tttaccctcc acataacgtg caaatcgtct ccctttttgt ccctctttcc taaagaacgt
     5161 ccattaagat aaagttctac ttcgtcggca ttattgtaat aagcccacac gtctatcgtt
     5221 tgaccttttt gccaattcca gtgtgggaaa acgtgtaaaa catctttgtc tgtccattca
     5281 ctttgataca tgtaataaat atcttttggg aagccggcca aatcgataat tccaaaatag
     5341 gaacttcttg atggccagga gtaaggagtc ggttccccca aatagtcaaa acccgtccaa
     5401 ataaattggc cagaaatgaa atcgtgtttt ttaactattt tccatgtttt ttcgtgggta
     5461 gagccccagg gaacatgaga attgtcatag gcagaacatt tttgcaaagg attgaaaaaa
     5521 ggtttatccc aactttcggg ccaaatatat atgctgtcgg atggcataag gtaaaatcct
     5581 cttgtttgta ctgccgatac agattctgta acaataaagg gtttgccagg aaagttttta
     5641 ggaacatcca taaattcgtt gtgatgataa ttgaaaccaa taatatcaag tcctccagac
     5701 aaaaaaagcg gattgttttt ttgggttcca ttgcaagctg ctgttaccgg ccttgtagga
     5761 tccagtaact taacaatatc ggaaagatgt ttggttagca aggaactttc tgaaatttta
     5821 gcttttgggg aatcggatat ttttaaattg tttagcaaaa gattcgcttg atcaagattc
     5881 atgttgtcgg tcgtaatgtt gtcttcccat tgttccatca cttcatttcc aatactccac
     5941 ataaaaacgg aaggatgatt gcgatctctt tttatccaat cggtcatatc tgttttatac
     6001 catttcggaa agtcttgagc ataatcataa gccgatttct ttttgaacca catgtcaaaa
     6061 atttcatcca ttacaatgaa tcccatacta tcgcaaagct gcaacagttc gggagcagga
     6121 ggattatgtg agcatctaat agcattgcaa cccatgtctt taagcagttg caattgacgt
     6181 tctaaagccc ttttgttgat ggcagctccc aaagcgccta agtcgtgatg attacaaact
     6241 ccgttaattt ttaaatgttt tccatttaat gaaaaaccct tttgagcatc aaaattaaaa
     6301 tatcgaaatc cgacagatgt ttcatattca tctgtctcct tgccttcaac tgaaagtttt
     6361 gtaatcattt tgtacaaata aggatgttca atatcccaga gaatcggttt attgaccgaa
     6421 aatgattgag aagccacagc tagcgaatcg ggtaaaactt ctatcgtagt ttcgtttttt
     6481 gtaattttcc gcccttcatt gtctaacaac atttgttcaa cttttacaag ccgtcgttcg
     6541 cccgttgaat ttcttactga agtttgaatt tgaatagtcg ctgcattttc gttcacaacg
     6601 ggagttgtaa aataagttcc ccacaaatcc acatgaatgg gttgtgttat ggtcagccaa
     6661 acattacgat atatgcctga tcctgaatac cagcgcgaat tgggctgctt ggaattgtca
     6721 acgcgtacgg ctaaggtatt tttttctcca aattttaaga aaggtgttaa ttcatagcgg
     6781 aacgagatgt atccataagg tcgtttgccg agataatgac cattgatcca aacttcacta
     6841 ttgcgataaa caccgtcgaa atcgacatat acgcatttat tttgctcgga ctgaggtaca
     6901 aagaaagttt ttctatacca gccaatgcca ccgggaagag ctcctccacc ggctgtggcg
     6961 gggaactttt cagaaaaatc ggattcaata ctccaatcgt ggggtaaatt aagagttctc
     7021 catgcaccat catcgaatgc cgggcttgaa gcatccattg cggaatcgct taaggtgaat
     7081 ttccagtctt gggtaaagtc gatgacttca cggagtgata cttgtcggct gcatgaactt
     7141 gcaagaatac aggtcaacaa taaataatga ataattttca taactataga ttttttttaa
     7201 ggttaacaat aataaattct gattttttct gataaactgg tgtctttttt ttaaatttaa
     7261 ctgctcataa ttagaacatt aattttaact gccatgaaac gtgataaatt aaaattcggg
     7321 tacccggcca aattcttttc gtaatcatta tcattgccca tgttccaatg tttgaagttt
     7381 tgcaattcaa atatacaaca atagggtgaa tgcttaagca tttgtcccat tcattttaca
     7441 tttttcttaa aaatgtttat cggtgaggag taatattttg ttacaatata attcttaatt
     7501 tactaattat taacttatta atcaattnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnata ttttgttaca atataattct taatttacta attattaact tattaatcaa
     7681 ttatttgttg aatcattggt tgtgaaaaaa aatattttat attacggatt ttttatatct
     7741 ttgacaaatt ttttcgatca aatccttttc gacagaatat gctaattaaa atttatgaga
     7801 ccaatcccaa tccccacgaa atacatcgtg taattgaaac tttacgcgac ggaggaacca
     7861 ttatatttcc taccgataca gtttatgcat tcggatgcag tttgttcaac aaacacggca
     7921 tagaatatat tgcacgcatc aagaaaaaag atcttcgcag ctcccaactt tcatttgttt
     7981 gtgaatccat aaaacaaata agcgaatata ccaaacttga tgacgaaacc tttaaactga
     8041 taaaaaaaaa tctgcccggc ccgtttactt tcattctcaa agggaatcat cgcctgccta
     8101 aactttttaa agataaaaaa acggttggaa tacgaatgcc aaaaaataat attgcactca
     8161 ccattgttca tgaattagga aatccattaa tgaccagttc tattttttac cacaacaatt
     8221 cactggaata tattgttgac cctgaaatga ttgaagaaaa atatggccat ttggtcgatt
     8281 tggtaattga tggaggaccc ggaggttata ttccatctac cattgttgat tgcacctgcg
     8341 aagaaccggt tattttgagg caaggcgctg gagaactgat ttggtaagta tttttttatt
     8401 tttttatgtt gctaatcaat aacacaaaaa cttaaagaaa acgaacaaga tggtattata
     8461 caattatttt taactcttcg aaacttatcg aaagcatttt tgcataagca ttgatgatca
     8521 tgatttgtac gatcagcatt ttacttcgga aagtattttt taagttacct cccctaaagc
     8581 ttattaaatt tttaaagaaa caaagaaaat tttctggcac aaacaaaaac ttgtaagacg
     8641 ctaattttaa cccccattac acattaacca atttaaaggg aaagccaaag tcagaagagc
     8701 tgttccataa tcaggaaaac caagctctgc ttatttaaaa caattttgag aaccagttta
     8761 ctgttgattt tttcaagata tgcatcaact gcagaagttc tttgtaaaag ttctatatct
     8821 tagataaagt tttctctgtt tttatacaag atttacacct ttttcctgtt gcggatgcag
     8881 cgataaaaag gaaactactc tgataaaact tcttttttgt tttaagacaa agaaatttaa
     8941 ggatggacta aagtttaaat ttttttaatg acggaagata ttgcaagaca actttagcta
     9001 atgtcgataa atttcagtcg atgttaaaag aaacctcaat gacatgataa ttaaaatacg
     9061 ggaaaagcaa gcaattaaaa cttaactttc aaaaaccaat catttttact acttttgcaa
     9121 attatttttt taaaactcat cgttaaatca tccaaaagca actgttggga aatggagatc
     9181 gttcaaatca aagataaaaa gttcaaaaaa tttattcctg aacaggaaat tctttcttcc
     9241 atcagaaaaa ttggtcagga aatcaataaa accctgaaag ataaaaatcc actctttatt
     9301 tctgttctca acggagcttt catgtttacg tccgacttaa tgaaagtggt gaacattccg
     9361 tgtgaaatca ccttcatcaa actttcctct tacgaagggc tttacagttc aggcagtgtg
     9421 aaagaagtga tgggattgaa cgaaaatatc gtaggccgta atgtagtgat tttggaagat
     9481 attatcgaca cgggactcac aatggaacaa atcattgatt cgctcaaagc aaaaggtgcc
     9541 ggagaaattc gggtggcaac atttttacaa aaacccgatg ccttgcagcg caacatcaaa
     9601 atagattaca ttgccatgaa aattcccaat gaatttatta ttggctacgg actcgactac
     9661 aacggatatg gaagaaattt aaaagatgtg tatactttga taacggatta atacagaaaa
     9721 cagattaaca aatgttgaat atcgttattt gtggagcacc gggaagtgga aagggaacac
     9781 agagcaaact gatagcagaa aagtacaatt taaaacattt atccaccgga gatatgctcc
     9841 gaaatgaaat taaacttcag acagaaattg gcaaaaaagc cgaagagttt atctccagag
     9901 ggcaattggt tcccgacgaa atgattattg ccatgctcga tcatgttctc gacaaggaaa
     9961 tgtaccatta caagggcatt attttggatg gttttccccg aacttacgct caggctgaag
    10021 ctttggaaaa actgctgaaa aaaagaaatg aagagattag tctgctcctc gacttgaaag
    10081 ttaacgatga agaattaatt agccgattgc ttaaacgcgc cgaaatatcg aacagaagtg
    10141 atgacaaact ggatgtgatt cggaaaagac tcgaaatcta ctatcaaaat gccgacgaag
    10201 ttaacaattt ttacaaaaaa acgaataaat atgtagccat agaaggcatt ggcacagtgg
    10261 aagaaatttt ttctcgtatc tctcaaatta tcgattcaaa aataaattcc tgataacctt
    10321 tgtctttcat taaattcctt tttccaaaat gtcaggttca aactttgtcg attacgtaaa
    10381 gatattttgc cgttcaggca aaggcggtgc aggttccact cattttcatc gggaaaaatt
    10441 tgctcccaaa ggtggccctg atggcggcga tggtggtcgt ggcgggcacg ttattttgcg
    10501 tggaaacaaa aattactgga cgttgcttca tctgaaatat gcccgacatg tatttgccga
    10561 agatggagaa aatggaagta aaaaccgtag ctttggcaag gatggaaacg acaagattat
    10621 tgaagtgccc tgcggaacgg ttgcgtacga tggcgaaacg ggcgagttta tctgcgacat
    10681 tcaggaagac ggtcaagagt ttgttttgtt gcaaggcgga agaggaggtt tgggaaattg
    10741 gcacttcaga agcgctactc agcagacgcc cagattttct caacccggag aaccgcgaaa
    10801 ggaaagaaca gttattcttc agttgaaaat attagccgac gttggtttgg ttggatttcc
    10861 caatgcggga aaatccacgc tgctttcagt cgtttctgca gcaaaaccag aaattgccga
    10921 ttatcctttc accacacttg tccctcaact gggaattgtg gcttaccgcg acaataaatc
    10981 ttttgttatg gccgatattc cgggaattat tgaaggcgca gccgaaggaa aaggactcgg
    11041 acttcgcttt ttacgccaca ttgaacgtaa ttccatttta ctgttcatga ttcctgcgga
    11101 agaggttgat attcgggaaa aatacaatat tttgctgaac gagctgaaaa aatataaccc
    11161 ctccttactg gaaaagtcga aaattctcgc catcagcaaa agtgatttgc tggatgcaga
    11221 actgaaagag gaaatgagaa aaaatttgcc ggccgatgtg ccgaccgttt ttatatcttc
    11281 acttaccggc gaaggtattc ttgaacttaa agatttgatt tgggatgaat tgaataaacc
    11341 ttccaaccaa ataattgaaa tttctcacgc tcccattccc atctcgaatc aggaaaatga
    11401 agaagaattg gaagaagact ttttccccga caacatttcg tctgatatgg aagaaaccga
    11461 ccgttttgaa aaaatggatt gggaggattg ggacgaataa tcttcgtttt ttgaaatttt
    11521 ctaagtttcc caccaatatt taaagatttt tcttgccttc aaaaaaggca tcagaaaaaa
    11581 ttttgtcatc gcctcagctt tttttgctat ttttgtaatg cctgagaaac gaaagttaca
    11641 gtatcttctt ttaagttccg ttgtcgaatt ctttaatttt gaaattatac tgacatgacc
    11701 aataaatgga tatttaagtc tctctccgaa gaagaaaaga taattcaaaa aaatctggta
    11761 gaaagtttga gcatcaaccc tacccttgcc caattacttg ttcaacgcgg tatccagact
    11821 tttgaagaag cacgcagttt tttccgtccc gatttggctg agcttcatga tcctttcctt
    11881 atgatggata tggacaaagc cattgaaaga ttgaacaaag ccatcgacaa caaggaaaaa
    11941 attctgattt acggggatta tgatgttgat ggaacgactt ctgtggcatt ggtctatcaa
    12001 tttttgaaaa aatattattc cgatatagat ttttatatcc ccgaccgcta taccgaagga
    12061 tacggcattt cgtttcaggg aattcaattt gccgctgaac agaatttttc gttgattatt
    12121 gctttggact gcggaataaa agccacagaa aaagttaaac atgcgctcaa tctcggaatc
    12181 gatttcatta tttgcgatca tcatactccc gacaaagaat tgcctcctgc tgtagctatt
    12241 ctcgatccca agcgtgaaga ctgcccttat ccttataaac atctttcggg ttgcggagtg
    12301 ggatttaaac tcctgcaggc tttcaccatg aaaaagggaa tcgatttttc agaactcaca
    12361 cctttgctcg atttagtagg actgagcata gcttccgata ttgttcccat aaccggagaa
    12421 aaccgcatcc ttgcctatta tggtttaaaa agacttaatt ccctaccttc attggggatc
    12481 cggagtatca tcaatgtatg tggacttgcc gacagggaaa ttaccattag cgatattgtg
    12541 tttaaaattg ggccacgcat caatgccagc ggacgtatga aatcagctcg tgaagctgtg
    12601 gaattactaa ttgccaacga tgcagaaatt gcacgacaaa aaagtggaaa tatcaatgaa
    12661 tacaacaacg accgcaagga tatagacaaa aatattaccg acgaagccat ttccatcatc
    12721 aactcgcagg atgtttacaa aaaacgcaaa tccattgtcg tatatcaacc tcattggcac
    12781 aaaggagtaa ttggtattgt tgcgtctcgt ctatccgacg aatactacaa acctaccgtt
    12841 gtcctcacgc gttcaaatgg cttgatttcc ggttctgctc gctcggtgcc gggtttcgat
    12901 ttgtacaaag ccattgattc gtgcagagat ttgctcgaaa atttcggcgg acacatgtat
    12961 gcagccggtc tttctatgaa ggaagaaaat attcaggctt ttaccgaacg cttcgaacaa
    13021 tttgtttcag aaaatattct ggaagaacaa acttatcctc aattggaaat cgatgctgtt
    13081 cttgaattta aggatattac tcccaaattt ttccgaatac tgaaacaatt tgcccctttt
    13141 gggccggaaa atatgaaacc tcttttcgtt tccaaaaaaa tctttgatta cgggacaagc
    13201 cgactggtgg gaaaagagca ggaacatctg agaatggaac tgatcgactc aagctcggaa
    13261 aacgtgatga atggaatagc ttttggtatg cacgaataca atgatcactt gaaggccctc
    13321 catccgcttg atatttgtta tacactggaa gaaaacaatt ttaatggaaa ttctaccatt
    13381 caattgatga tcagagatat aaagaccgaa gaatattaat tgaaacttcg ctcgaaaaga
    13441 ctgtgcttta ataagtttaa tcttgtttgg gctcttctga aacacattct tccgaagaaa
    13501 caggcgagac cgtctgctcg ggctgcggaa cattgggatg ttcaatattt tcgcccttta
    13561 taatgaagcg tttataataa gacaatatca aagacgtgaa gggcagtgca atgatcatcc
    13621 ctgcaaatcc taacagcgaa ccccaaatgg acaaggaaag caaaatcaaa gccggatgaa
    13681 gtcccgtata ttttcccatg attctcggaa ctaaaattaa atcctgaatc gattgcacta
    13741 tcaaccaaac tgctgcaatg ctgagagcaa tcatccaaaa attctgattg gtttccgaag
    13801 ctttcagcaa agccataaaa gcacatggaa taatacccag caactctaga tagggaacaa
    13861 gattgagaat accgatgctc aaaccaatca caattgccaa aggtaaacga atgatactca
    13921 gcccaatcat aaaaagaatt cccacacaca tggctattaa agcctgccct ctgaaataac
    13981 gattcatccc gtcctgaata tcctgaacaa tattggtaat caaggggcgg tatttcatgg
    14041 gaacaagttc aatccatttt tcggaaattt tctcgtaatc cattaaaata aagaatagat
    14101 aaagaaaaat aattactatc acagcaagtc ccagaataaa attcaaagaa tttccgacaa
    14161 aattccatac ttctggcaat aatttttgaa caagggacat aaatttttcg ctgcctaaaa
    14221 gtgtttgtaa atcaagttgc ctgaaatatt cagacaactg attttgccag gcaatgggca
    14281 aaaaagaatt tacatctaca tttcttaaaa aagaattgat aagcaccacc gctttttcta
    14341 tttcattgat gatcatcgga atcaacagaa aacccagacc cgtgagaata attatgaaaa
    14401 gcagcaaggt tgcaaaaata gctacaaaac gatacttgaa tttaagtttg tactgaaaaa
    14461 aatggactat aggctgaagc agataggcga gtaaccatgc aatcaaaaaa ggaagcaaaa
    14521 caccgcttaa tcgctgggtt aagaaaaaaa gagcggtaag aacggtcagc gtgatgacaa
    14581 gacgcaccac ccgatcaaat gtataaggtc tttcagtttg ttccatcata ttgcagaatt
    14641 atagaaaagt taaattttca tttgactgcg ttgaatttca ttaaaacagt tacgacaaat
    14701 tggttcatat tcctgcattt cgccaatcaa aacccgcttg ttgctgtcca ccagtcgatg
    14761 cgaaacataa gccaatgcac cacatctcac gcaaatggcg tgaaccttga acacatcttc
    14821 cgcaatagca cacaatgccg gaataggacc aaaaggtaca ccttgataat ccatatcgag
    14881 acccgccaca atgacacgaa tgccctgatt ggccaattga ttacacactt ccaccagccc
    14941 atcatcaaaa aactgtgctt catcaatacc cactacgtcc acatttgcag acaacaagag
    15001 aatacttccc gacgaaccta ccggggttga acgtatggca ttgcgatcgt gagaaacaac
    15061 ttcgctttcc gaataacgcg tatcgataac cggtttaaat atttctactc tttgcttggc
    15121 aaactgcgcc ctctttagtc gccgaatcaa ctcttctgtt ttccccgaga acatcgaacc
    15181 acaaatcact tctatactac cacgtctcga tgcatgagaa gtctgttctg aataaaacat
    15241 atcatttatt ttgttgaaat ttacgctttc tttgtatatt ttctatttca attattgtaa
    15301 ctttaccgtc taaaacgttt taaagacaat ttttttatag tctgaaaaat ttcaggttgg
    15361 aaaaataata tcttggacaa aattaaaaaa aataacaagt actgaaattc ttttaaagaa
    15421 aaaaatatga acaaagattt aatattggct ttgctccaca aaaacattca ggatttggac
    15481 atgctcaccg aaagtttcat ggaaatgaac gaacttccac cggccattct tcatctcgcc
    15541 atgcaaaaag ttgatgaaat tcatcaatat ctcgaagaac tgaatgaaaa caactcaacg
    15601 aaaaaacctg ccggtaaaga aatttttcct tcggaggatt tgaaagacaa aaaagaaaac
    15661 cactttctac atttttctga acattcgaca aatgattttg cagagccctc agaaaatgtt
    15721 tcgcagcaaa ccgaaattga accaatacaa gaaatggaat ttcaagtcgg cgaagaaaat
    15781 attgcccaag aaacagaaaa aacggaattt tctttcactg aaagtccaat taatgaaaaa
    15841 ctaaccgaat gggatgaaac agaaaaagaa acagcagaaa ttgagcaaga accgctcgaa
    15901 aacgaaaaaa accgatttgt ttctacccag cccgaaatag gagatgaaac taccgaaata
    15961 acagattcgg aagcccagga aaaggaagaa ccatcggcca atgaaggcca aaaaactgca
    16021 ccgacttcaa tcccgctgca ggaaaaattt tccaaaattc ccgataactc tttggggact
    16081 acatttgcca atcaaaaaat cgacgacata aaaaaagcca tcagcattgg cgaccgtttt
    16141 cgatttcagc gtgaactttt cagggggaat ggcgaagata tgaataaaac actgaattac
    16201 ctcaatcagc tggcaactct ggaagaagct gtttcttttt tgcaagccaa atacaaatgg
    16261 gatggagaaa acgaaacggt gcaggatttt tttcaaattc tcaaacgaag atttttataa
    16321 tttcatcaga atataattta ttttttaaag gtcttatgga acccacatgt ctgaatatta
    16381 tactcattcg tgtttgtgta tgcaatacat gtgggtctca tctgaaaaga atttttttta
    16441 aaaaatcatg agtcagcaaa agaacaaaac ttcattggcg gggacgacaa tcaataattt
    16501 ttccgcatcc aaagaaggag gtaaacttta tgtggttcct actcccatag gcaatttgga
    16561 agatatgaca tttcgcgccg taaaaattct caagaaagtg aatcttattt tggctgaaga
    16621 cacacgcacc agcagttttc tgctgaaaca cttcggcata gaaaccccca tgcagtcgca
    16681 tcacaaattc aacgaacaca aaacggttga aaatattgta caacagctga aaagcggcca
    16741 aattttagcc ctgatatccg atgccggcac tccggcaatt tccgacccgg gttttttgct
    16801 ggtacgcgaa tgtgtagaaa atggcataga aattgagtgt ttacctggag caacagcttt
    16861 tgttccggct ttggtaactt caggcttgcc taacgatcgc ttttgttttg aaggtttttt
    16921 gcccgtaaaa aaaggacgtc agcaacgttt aaaagaattg gaaacagaaa aaagaacgat
    16981 gattttctat gaatcgcctt tccgcttgct gaagactttg acacaactat cagaagtatt
    17041 tggcgaaaac cgtaaagctt ccgtttcacg tgaaatttcc aaaatgtttg aagagacagt
    17101 cagaggtacg ttagccgagt tgatacagca ttttactgaa aaaacgccaa aaggagaatt
    17161 cgttttgata gttgccggtt ttgaagaata gctgtatctt tgcacagtat tttaaagtgt
    17221 aaaattttta ttttaattta ttgattatca tattattaat ttttttcatt caacaattcg
    17281 tattttctcc atactccaaa ccattataat tttccatccc catgaacaaa aatatttcat
    17341 accgcaacat cagagttttt agcgtaattt ttctggtagc ttttctgact gtttcgtgtg
    17401 gaaaacaatc gtccgattac aaaaagctca aggcagaaaa cgactccttg ctgaaagaaa
    17461 aggaaagact cgaacgcgaa gtagatgact atttttcgag catcaatcag attcaggaaa
    17521 atctggaaaa aattaaaagt accgaaaatg ttatctcagt tgaaccggca ggagaagaat
    17581 tatcacagga tgcacgcact cgaatcaacg aagacatcaa ttaccttaac gagttgctgc
    17641 aaaccaacaa ggatgagctg gcacggctga agtcgagatt gagcaaaagt tcattaaaac
    17701 tcaccgaact ggaaagaacc attacccgcc tcacaaaaac attggaagaa gaaacggcgc
    17761 gcattgcttt tctggaaaaa aagctggccg aaaaggacac ccttatagct caaatgggca
    17821 gcaccatcag ccaaatggga gaaaatatca atacactgac taccgaaaac aaatctaagg
    17881 aagaaaaaat taaacaactc gatgaaaatc ttcattcggc atggtatgtt tttgggtcac
    17941 gaaaagaact cattgatcag aaaatactta catctaaagg aattttcaga aaagccgaag
    18001 tattaaaaga ctccttcaac aaggattatt ttgtcaggat tgatgcccgc aatgccaaaa
    18061 ccattccgct gtatgcttcc aaagctaaaa tactcacaac acatccgact tcatcttata
    18121 caattgaaaa acaagatggc aagttggtgc tcatcattgt cgatccgact gctttctgga
    18181 gtgtgagcaa gtaccttgtt atcgaagttg attaacaaca tttttttgtc tgaaagaatt
    18241 ttaaaaaaac aggcgtcgga aaaacttcaa gtctttttta ggctgtcgga ataatcaagt
    18301 atgattttta gaaatttatt tctgaaaaat gctaccgaat gaataacaag catccttacc
    18361 gatacatttt tctcgatttg gacgacacaa tctgggattt tcatgccaac gcccgactgt
    18421 cgttgcaaaa aatgtacgaa cagcagacgc tcaaccgtta ttttgaaaat ttcgacgaat
    18481 ttttccgaat ttatgccatg cgaaatatgg aactttggga aatgtacggg aaaggagaaa
    18541 ttaccaagga ttttttgatg atcgaacgtt ttcgctatcc gcttgccaaa atgggacttg
    18601 acgacttgca actggctgaa gccattggaa aacaatatct ggaaattctt cccgaacaga
    18661 cgactctgct tcccttcgcc cgagagctgc tggattatct ccattccaag tatccgctga
    18721 cactcatttc aaacggattt gtagaagtgc aatacaaaaa actgaaatcc tgccaaatcg
    18781 aacattattt ctctcacgtg gtactttcgg aacaagccgg cgcactcaaa cccgacccag
    18841 aaatattcaa ctacgctctt cacctgaatc aggcctcacc cgaagaagcc attatgattg
    18901 gcgacagttt ttcggccgat attgtgggcg ccaaaaatgc aggaatcgac cagatttatc
    18961 ttcaaaaaaa caatatgctg ccttatacca aacaacaggc cacttttatc gtaaaatcgc
    19021 tcgaagaaat tttttcaatt ttgtagcaga caaaattccc tgcatgttta tctacaattc
    19081 aaaaaaagct caaaaaattt attttttgac gaaaaccaaa aaaagcacta tctttgaacg
    19141 tttttaatag ttaaaaccat tgcatcatga atacaaacga agacaattta ctcgaataca
    19201 gcgatgaaga agccgtaaaa ttcattctaa agtctcttcc ggccgaagtg aaagaccgca
    19261 tcgacgaaga tactgttcag tacgtgttgg atgtgattta cgatttttac aacgaaaaag
    19321 ggtatatcga cgaagatgat actgccgaag aaaccagcat tgacgaagaa gaaatgtatg
    19381 aatacatttt ggaaaacgcc ttgaaagatg aaatcaacct tacggaagac gatattcaga
    19441 tgattttgga tgctgaattt gattacggca aatcattggg tatttacaga gaagaataat
    19501 ttcgtttttc acatacctac tttttccctc aacaccaacg catgcaggtt ttgcttggtg
    19561 tttttttatc tttggtattt ttgtaaaata acaaaaaaca tatgttttat gtgatttatc
    19621 cgcttttgtg gttgttgtct ctgcttccgc tgaaagtgct ttatgccttt tctccattaa
    19681 tatatttcct gatttatcgt gtagcaggtt acaggaaaac agtggttcga caaaatcttc
    19741 aaaattcatt tcctgaactt tcggcatccg aaagaagaaa aatagaaaag aatttttacc
    19801 gtttctttgc cgatttgttg ctcgaaatcg ttaaacttct tcacataagc gacaaagagt
    19861 tgctgcaaag ggtgaaattt gtcaatgttg aggagttttt tgaatgttgt cgtcagggaa
    19921 aatatccaat tttgatgctc ggtcattatg gcaattggga atggatattg accatcagtt
    19981 tgttaatgcc cgaaaattgc gagtctttta ccgtgtacaa gcggttaaaa aacagaaagt
    20041 tcgatcaatt catgaagtcg cttcgtgaaa aacggggagc agtaatggtg gagaaacgca
    20101 atctggcaag gaacatgata cgttgggcaa aagaaggaaa atttggtgct tacggattta
    20161 ttgccgacca aagtccgagt ggaaaaaatc ttcgaaattg gacaactttt ttgcaccagc
    20221 cgactgcaat tttgcccggc gccgaacaat tggcaagaaa attcaattat ccggtttttt
    20281 atgcacgcgt gagccgacca aaacgtggat attattcctg tgagattttg cccatagccc
    20341 gagagccacg aaccacttcc gacaatgaaa tttctacacg tttcatgcaa ctgctcgaag
    20401 aagacatctt ggctcatcct gaactttggc tttggtcgca tcgccgatgg aaaattaaac
    20461 ctctgattga tgaggtgaaa aaagataatt gacaatcgct ttcgctttga cacaaacatc
    20521 agctgttgca gcaaaacgac agattttgat tttttttctg aacgtctgaa tgttaatcga
    20581 cattttaaaa aaaatttttt aaaattaaaa ccgcaaaaaa caaataattc ttttatagtt
    20641 gactatgcaa gtttcggtag tgatattgaa ttggaacggg aaaaattact tgcagcaatt
    20701 tcttcccata ctgttggaaa agacgcgttt gtcgtatgtg gaaatagtgg tggccgacaa
    20761 tggttctaca gatggttcac ttcagttgtt gaaagaacag tttccaatgg tgcgcatcat
    20821 tttattcgat aaaaattatg gttttgccga aggctacaat aaagccttgt cccaaatcaa
    20881 atcggattat tacgtgctgt tgaattccga tgtggaagtt acagacaatt ggttgctccc
    20941 gctggtggat tatatggaaa aacatccaca aactgcagct tgccaaccaa aaatactttc
    21001 ttattttaat cgaacacatt ttgaatatgc aggtgctgcc ggcggtttta tcgattatct
    21061 gggatttgcc ttttgtaggg gacgcatttt tggagaagtg gaagaagaca agggacaata
    21121 tgatgacccg atgaatgttt tctggacttc cggagcctgt atgatggttc gcgcagggct
    21181 ttatcataaa atgggcggat ttgatgcaga tttttttgcc cacatggaag agatagatct
    21241 ttgctggcga ttcaacagtc gtggctatca attgacctgt gttcctgaaa gcatggttta
    21301 ccatatcgga ggaggcactt tgcaatcgga acatccattc aaaacttacc tcaactttcg
    21361 gaataatttg ttgatgcttt ataaaaatct accgaaaaag caattaaaaa gcgtgttttt
    21421 caagcgagcc attctcgatt acctggctgt ttttcaattt cttgcctctg ccaaacccaa
    21481 aaatgcacga agcatactga aagctcgctc cgatttcaga aaaatgaaac caaattttct
    21541 ggataaaaga aaggaaaata tcctcttctc caccgcaaca tcctttccca tgatttatcc
    21601 ccgaagtatt gtggtggatt attatttgag aaagaagaaa aaattcacaa cactaaaatt
    21661 cgatcaaaaa ccaaactaaa aaatatttta aaaactgatt cttaagcttc cacgaatagc
    21721 aaaattgtcg ccggaaaatt gcttaatatc ggtcaatcgc cagattgcct gtaaattgaa
    21781 tagatgcaga atattggcca gtccgacgcc aacttcggtg taaggacgat taaaaggata
    21841 aacgtaagcc ggaaaatcca tgaccgacaa gtggtcgtta ctcatttttc cgtaaccgaa
    21901 cttaacggaa aacatttcgc gcatgttcag gtacttgagg agcggaatat gatttaatat
    21961 cagcccgttc ataatcagtt ctccgtgaaa atcaaaatat ttgtcggctg caaattgcat
    22021 aaaattcatc atgctgaaac ggtaaaatcc gtatccgcct tccgctccgg gttgatatgc
    22081 caacagtgga taaggaacgc ttccaaaaat acttccggct tccatccaat aattaacttg
    22141 tcccaaatcg aactggacat tttgtttaag aatacccgaa acttttccgt agtatttttc
    22201 agtatttcca agttcatatt tccccacttc caaaatgccg taaattattg ggttgcggtt
    22261 attgatataa attcgctgta aatggtcttc gtattttttt tcgttgaacg aaaaacgtgt
    22321 tgaaagggta aaagattgtt gttggaaatt gttaatatcg actccgtcct tttttaacgg
    22381 taaagcatcg gatggaagct gctgcaaaga cctgaaatac aatccggatt ctatattcct
    22441 gttccagtcg ccggagaaag aaagcgtcca ttcttttctt tcgttcaatc gaccggcact
    22501 gcgaagggca aaaatggtgc cggcaatatc ttcatcgcca cttacaagcg gattttcgag
    22561 atacagaaaa ttattgtaat tataatctat acgacggtaa tcgttcgtgt aattcaaacc
    22621 gataatgcgt cttttttccg ctggcaattt atattgtgtg aaacctgaat attttatggc
    22681 ttcgtttttg aatccgtaac ccacaaaacc gccaacagta aagtttttcc acattttttc
    22741 gttggttctg aagggcaatg tcagtctcag tccttccaca tcggtagtgc gggcaagata
    22801 ttgaattttt cctatatcaa tttttcctac aggaatgtaa cctgtaaaaa taatgtcggc
    22861 caaccattta gctgcattca tcaggggtgt ttttcctaat tgttgaagct tctgattgag
    22921 agtttcttcg ctgtacccgc tttgagcaaa actttcggga atttggatac ttccggtatt
    22981 gtaaattgta cttcttttaa agaaaatctg agaaccggcc ggaacactgt cggttaaaat
    23041 gtcaaggttc aataccagtg aaagctgatc tttgttacgc acccagcgtt ttgacgaaaa
    23101 gggcacaaat tcctgcttta tgctcaggtg tcgaataaag ttgatgttgg cttccgaagg
    23161 gagttctgct tcaatgtagg taagtgccag cgaagccgaa tcgagacaga attttccatt
    23221 gaaagccgga tttctcggat ttcggcttct gaaatgtatt tcgtattgtt ttcccgaaga
    23281 tgtaaccatg ctgtcggcaa ggtaataatt gtaatacata ttgccaatgc ttgccagtgg
    23341 gctgataatg tttttattga aaacaggaat ggaattttcg taaaaattca gtgatggatt
    23401 gagttcgcca aaaagctgtt ccaaaatttt gttgccttct tcagaggaag aaaagatttt
    23461 ttttgaaagc tctgtttttg cagtggaagt caattgatat tttatttcgt ccatgtaaag
    23521 cggaacaagc aaagaagaat cggaagagag cagatttcct tcctgaagct ggcggaaaat
    23581 ctgtttgctg atggagcggt gttgcgaacg agagagaaaa atagcatttt gttcggttgc
    23641 caaaacttcg taatgaggca gtttggtctc atcatttact tttctcaaca gtcggacttt
    23701 tctcattaaa tcgagagccg gattagcgcc gggcaaaata aaaacttcct gaagcagtgt
    23761 gttcgattct tccatctcaa cagtcaggcc aatatttgaa cccggtttta ttttgatttc
    23821 cgtctttttg tatccgatag ccgaaaatac gagtatattt tcctttcccg tgtgtctaag
    23881 aagaaaataa ccttcttcgt tgcttgttgt tccaacggat gttcctttga agtaaatgct
    23941 gaccgatgaa agcgggtgct tgtcgtattt gtcgataacc tgacctacca caattgtttc
    24001 ttgagcctgc aaaccggcaa cgaatagaac ttctgtcaag agaattccca tcataaaacg
    24061 gaaaagcaac aaactttttt cagaaatccg acagattttt attttcctca tttttcgatt
    24121 ggttcaaggg atgatatttt aaaatggttt ctctaaggaa ctgcacgtcg atatgggtat
    24181 aaagctcggt tgtagtaatg gattcgtgcc ccaacaactg ttgaatggcg cgcaggttgg
    24241 ctccattttc cagcaagtgg gtagcaaaag aatgacgaaa agtatgcgga ctgatgtttt
    24301 tttgaatgcc tgccatagtt gccaaatcct tcacaatttt gaaaatcatg gctctggtca
    24361 gtttttcgcc tcttcgattc agaaaaagaa aatcttcatg tccttttttg atttccaaat
    24421 gattgcggtc gattttccaa agttcgattt gctttatcga attgggcgaa agcggaacaa
    24481 gccgctgttt actccctttt ccctctacca tcatgtaccc ttcgttaaga taaagctgcg
    24541 aaagtttgac gttaatcaat tcggaaacgc gcacgcccga accgtagaga acttcaatga
    24601 tggctttgtt gcgttgacct tcaggttttg aaaggtcgat ggctgaaatc atggcatcaa
    24661 tttcttctac cgacaatatt tgtggcaacc gaagtccgat tttaggactt tcgagcaatt
    24721 cgctcgggtc agtagaaatt atatttttgt aaaccaaaaa acgataaaac gaactgatgc
    24781 tcgatatgat gcgggcttgt gaacgcggat gaattcccag actaccaatt tcgattatga
    24841 aatcacgcaa aagctcgatg gttgcatctt gcaacaatac atgaaaatcg gacagatagc
    24901 tgttgagttt ttccaaatct ctttcgtaag cctgaatggt attttcggaa agtgcttttt
    24961 caaacttcag ataataatga taatcggtga gtaaggagtt catagacaat ttttaaaacc
    25021 gttgcaaaga tacaaaatct cgaaggaact tttgtaggaa agttaaaact acatttttat
    25081 gcaaaatatt taatttttct ccattttgtc aaacatctta aaagtttttc taattttgtc
    25141 ctccaacttg ttccatcttt tcgattgttt aatcaatata ctaaaaatca atatgctgtg
    25201 taaagtaaaa actttatttg tcattttatt ttgtgccatt aattttgctc tttcagctta
    25261 tgatgtaaca gggcatcgca ttattgccag cattgcctat cagaatctca atgcagctac
    25321 acgcaatcgg gtggacaaat tgcttggtaa gcagggaatt gtttacgaag cgggttgggc
    25381 tgacgaaata agaagcgacg ataattatgc ctattcttac cagtggcatt atcagaactt
    25441 gaaagacagc cttacatcga aagatttaaa gtttttgctg gaacatccca aagtcgaagg
    25501 caaacatttg ttttttgcgc tcgattcgtt gtccggtgag cttaaaaaga atcccaacaa
    25561 tgcggaagct ttaaaattta tcattcattt tgtcggcgat ttgcatcaac ccatgcattt
    25621 gggacgtgtt gacgatttgg gaggaaacaa aatcatggta aagtggtttg gagccaaatc
    25681 caatctgcat cgcgtgtggg atagtcagtt gctggaaagt tccaagtatt cctattctga
    25741 atacagtatt tatctgcaaa acaagtttga accacaaaga gccacgtatc aaaaattcag
    25801 tttgttgcag tcggcagaag ctgtatatgg gcttcgcaac cagatttatg cttacgattt
    25861 gacaacagaa acgaataact acaagtacat ttaccttttc tcggataaac tggatgaaat
    25921 gctttattta gggggcattc agctggcaaa tgttttaaac ggaatttact cgaaataaaa
    25981 aaattattga ttgtaaggaa gacaacgaca agcaggtgtc agactgtcaa agaatttata
    26041 actgaaagta atgctgagat tagccaaaag atcattctct ttggaagtaa cagtagaaat
    26101 accatctata aaatctgtaa aagcataatt ccaggatagt tcggcaccaa tagaaaattt
    26161 aggtgtgatt gaatatttat atcctattcc aaatggaata aaagctgctg tttcattgag
    26221 ttttatctta tcgtatatcc tgtctggcgg aggttgagaa atagccactc catttttctt
    26281 tacttcagca ttactgttca tcacacctat gcccccaaaa agataaagcg aataataatc
    26341 gtagtaattt gaatgtatgc ctcccaaaaa agtatattcc gcttgcaacg acaagcttga
    26401 aacttcagaa ttaaaggaat attgtctggc ttcgtttctg gaatttttat cgttaccggt
    26461 aaagttcccg taattaaaaa tcagtttata accaatatta tgtttaaatg tctgaccgta
    26521 gccaagcgaa aacaaaaatc gggtattcaa cacatcccaa tcgttaatac cgtttaaaaa
    26581 ctgctcataa tgcgaaccac caacgtctcc aaagcaataa gcaggcccgg cagcagcaaa
    26641 aaaaattgat tttgagttgg gaattacact tttacctatt aatccatttt tctgtgcatg
    26701 gaccacgaaa aatgaaattt gtacaccaaa aagcaatatt atgatcaaaa cttttttcat
    26761 gggtgtgttg gcaatggatt ggttgcaaaa ttaaaaattt ttttagtatg aaacattaaa
    26821 aacaattttt ttagtgtttt cttagtattt tcttaatacc tgaaactgct tcccccaaca
    26881 aactcacgca aaagtgaagt aggaggaatt tccctgtcgg gaataggtac aaaataattt
    26941 aaaaatttaa tgagccgatg agcttcggtg tattcgtcgc aatattttgg gacttcggca
    27001 agcaaaggtt cagcatagcg ttttaacacg gcaaactcaa aaagcaaggc agagttgaac
    27061 atgacatcgg cattttcctg aaacgggaaa atccattttt cttcaccttt tctgacgctt
    27121 tcccaacgcg caatggtttc ccttgcggaa taatttctgt accttgaatc gcgaataatc
    27181 cttcgcaaca atcgagtgtc ggttgtaggt atccaattgt gattgtcgat ggagatggtg
    27241 gtcagagccg aaacgtatat tttgaatgta gcttctgctg gaatttcggg aagaagtttc
    27301 ggattcagtg catgaatccc ttctaaaatc accagactgt cttcagcaag tttcagttta
    27361 tctccacgat aatagcgccg accctcttca aaattgaaac tgggaagttc aatttcttta
    27421 ccttcaatca gttctcgaag ttgttgattt aacaactcta aatcaagtgc atgcagatgc
    27481 tcgaaatcta actctccatt ttcatcacga ggtgttttgt cgcggtcaac aaaataattg
    27541 tccagcgaaa ttgctaccgg tttgattcta caaacggcca actgaaccga aagtcgctta
    27601 ctgaaagtag tttttcccga agaggaaggc cccgaaataa gtataaattt tactttgtct
    27661 ttccgttggg caatggtgtc ggcaatagta gccacctttt tttcgtgtaa agcttcggaa
    27721 atttttatca gtgaaaaagc ctgcttatta cgacaagcaa cgttgaattc acctacattg
    27781 ctcagattca tgattttgtt ccattccaca tattctttaa atatatcata aagttttggc
    27841 tgtggaacaa tttcttccaa ttctgttgga ttctcccgat tcggaacacg caacagcagt
    27901 ccgtcgtaat aaggaatcaa atcatacagg ttcaaataat ctgtcgaagg cagcaggacg
    27961 ccgttgtaat aatcgatata atcatcgatt ctgaaatatc ggcaatacgg attacctaaa
    28021 gtctggaaga gaacaatttt atctttttgg tcagcaaata gcttgattac ttcagaggtt
    28081 tgtttttctt cacaaattat ggttttacct tgagagataa ttaaattaat tcgttcttta
    28141 attttatcga ccaagctttg tgtgagaggt tcatcgatgt tgttcagtac gcaataataa
    28201 cctttcgata aaggatgttc gatgcgaatg ttgcctttgg ggaataattc agaaacggct
    28261 ttggcaacta ccattgtcag cgtgcgaaca taaacgcgca tgcccgaaga ggtgcttaaa
    28321 tcaatgaact caatgtcttt gggcttataa atcaaaaaat ttagatcttc gaccttataa
    28381 tttacgcggg ctgcgacaac ctgatatttc aattgaatgt ttaacgaccg gtaaatttca
    28441 attagtgaag ttcccaaggg gaaatcgtga tatgaacggg tatttttgca ataaatggtt
    28501 acggtttttc ccatgagtaa gataaaaaaa ttgttttcaa tccaatttaa aatatgtcgg
    28561 taaaattata tatttttttt tgcatttctt tcaagaaaaa attttttaaa gtacttttgc
    28621 aaaaaaatct gtaattagaa ataaaatgca ttacacattt tatgatttac ttacattaat
    28681 tggttcattg gggttgtttc tttatggaat gaaaaccatg agcgaaggac tgcaaaaagc
    28741 taccggcgac acgctccgtt cgataatggc tgcaatgaca agaaatcgtt tcacggggat
    28801 tcttaccggg ttgcttatta cctcacttat tcagtcttct tctgccacta ctgtaatggt
    28861 ggttagtttt gtaaatgcgg gattattgtc tctttatcaa gcaataaccg tcattatggg
    28921 agcaaacata ggaacaacca taacggcatg gatgatttcg atttttggat ttaaagttag
    28981 tatcgctgct ttttctattc cattgatagg catttccatc ccgcttattt tctcctctaa
    29041 aagtcgcaat aaatccatag gcgaattttt aattggattt gccttattgt ttatgggttt
    29101 ggagttattg aaaaattcag tccccgatat tcagggcaat ccggaagtct taaattttct
    29161 tgcaaaatat acaaattttg ggtttggttc gatattactg tttttactca taggcaccat
    29221 gctgaccatt atagttcaat catcgagtgc tactatggct ataacattaa tcatatgcag
    29281 caaagggtgg atttcttatg aacttggagc tgccatggta ttgggtgaaa atataggaac
    29341 aactattacg gccaatctgg cagctattcc tacaaatgtt tcagctaaaa gagccgcctt
    29401 atctcattta atcttcaaca tttttggtgt tatttggatg ttgatcgctt tcaggccatt
    29461 catcaatttg gttacaatgt tgataactca atttggaccg ggcgacccta ctcaattaac
    29521 tgctttctcc aatagccttg acccagatac gctaaaagtt atttcttcgg gaagtgaaaa
    29581 tctttcaccc gaacaaattg cagtcaggaa tcaactagaa aattatcaaa tagcaacctc
    29641 atacggcttg tcattatttc ataccttatt caacataacc aatactttgg ttttgatatg
    29701 gtttgctaaa attatagaga aaatttcaac cttgctaata aaaaagaaag atacggatga
    29761 agaatttcag cttcaattta tttctaccgg attgctttct acttcagaac tttcacttat
    29821 acaggcatgg aaagagatta aggtttatgc tgaacgttcc caccgtatgt tttcattggt
    29881 aagagaattg cttcaagaaa aaaatgaaaa tgattttata aaaaaattta gtcgagttca
    29941 gaaatatgaa aatataagcg accgaatgga agttgaaata gcccactacc ttacgaaagt
    30001 ggccgaaggc aaattaagtg aaaaggggaa atttcagata caaaccatgt tgaaagtaat
    30061 ttcggaaatt gagagtattg gtgatagttg ttacaacttg gccagaacta tcaagcgaaa
    30121 aaaagacgac aaagctgaat ataccaaaga aatggatgat aatttgaatg aaatgatgaa
    30181 tttggtagaa gatgccctct tacaaatgcg agcagctatc gacaacaacc atgtatcaaa
    30241 agaagaattg aatcgcgctg aaaatttgga aaacgaaatc aataatttca ggaatcaact
    30301 caaaaataaa aatattgatg acgttaaaga aaacaaatac gattatcagg caagcgttac
    30361 atacatggat atgcttgaag aatgcgaaaa acttggagat tatatcgtaa atatagttga
    30421 agcactttac gaagcttcca aataaatgac gaaaggaatc aaattccatg tttaatccgt
    30481 ttccatctct gccgatccac caaaagtaat gttatccatt tttctgcctc gcacaaaatt
    30541 tcttttcata aaaaagccgt aattttgctt ttgaaattga caagtttttc aactctttct
    30601 tttttatatc aataacttga aaagtcgaac gaaaaaaatt atcgtctaac actactctca
    30661 tttaaaatgg attacatatt gattatttcg ggttttgctg ttttggtttt gggtgctaac
    30721 ttattagttg atggtgcaag cagcttggcc aagaaattaa agatatccga tttgatcatc
    30781 ggacttactg tggtggcttt tgggacttcc gcccctgaac tggtggtaaa tgtaatagct
    30841 tctgtgaaac ctaatgcaac agatatagca atttccaatg ttattgggag caatattgtt
    30901 aatatcttta tcattcttgg attttcagct ctgtttcatc ccattaagtc ggagaaaact
    30961 acaactttat ttgatatacc cttaagcttt tttgccattg ttgttacata tcttcttgct
    31021 tttacgggag ataggatttt atcaagaacc gatggacttg ttatgcttgg tttttttatc
    31081 gctttcatat attttaccac ccgcaggtcg ataaaacatc cattgatacc tttggaagaa
    31141 aacatcaaaa ttcgtaaaac gggaatttcg attttgatga tttcttgtgg attggcagcc
    31201 cttataggtg gggctgaaat tattgtaccc agcgccattc gtatagcaga aaatttgggt
    31261 atcagtcaag cagtcattgg attgaccatc gttgcattgg gaacatcgct ccctgagttg
    31321 gccacctctg tagttgctgc tatcaaaaag aattccgata ttgcacttgg gaatattatt
    31381 ggctcaaata tttttaatat tttcctgatt ttgggtgtga gcagtgttat tcgcccatta
    31441 cccatctata ggggcatagg aatagacttg actatgggtg cacttggcag tgtgatggtt
    31501 tttgtttttg tttttggaag taagtcccac agcataaaaa aatggcaagg tttattgctg
    31561 attttcatat atctaatgta cgcaattttc aaaatttttt aatttaaagt atccttaaaa
    31621 aattatttaa caactcctcg ttatgaatct ttttgatgtg tatccgcttt ttgatataga
    31681 aattgttaaa ggacagggat gtcgtattta cgacaaaaac gatacggaat atctggattt
    31741 ttatggaggt catgccgtca tttccattgg gcattcgcat ccctattttg taaaaaagct
    31801 ggcggatcag attcagaaac tggtttttta ttccaattct gtgaaaaaca gtttacaggt
    31861 tgaattggcc gaaaagctgg gaaaaatagc tggttacgat gattattccc tgtttcttat
    31921 taattcgggc gcagaagcaa acgagaatgc gttgaaactg gcttcgtttc acaatggacg
    31981 cagcaaggtg attgctttcg gaaaaagttt tcatggcaga acttcggctg cggtcggagt
    32041 tactgacaat ccagccattc aggcgcctat taatcgaaat tttgaggtag aatttctacc
    32101 gttgaacgat atagaggcag ttcttcaatc tttgaaaaac agagatgttt gtgcggtgat
    32161 tattgaaggc attcaaggag taggagggat tcagattcca caaccggcgt ttcttgaagc
    32221 gattagcgca gcctgcaagg agaccggaac cgtacttatt ctggacgaga ttcaatcggg
    32281 ttatgggcga agtggaaaat ttttcgctca tcaatatgcc gatattcgcc ccgatattat
    32341 taccactgcg aaaggcatgg gaaacggagt tccgataggt ggagtaatta tcagcccaat
    32401 gtttaagcct acttacggaa tgttgggaac cacttttgga ggaaatcatc ttgcctgtac
    32461 cgctgccatt gctgtattgg atgttattga acaggaacat cttatggaaa atgccttgca
    32521 ggtgggaaat tatttgataa acgaactcca aaaattttcc cgattgaaag aagtcagagg
    32581 aaaaggtttg atgataggga ttgaatttga cgaaccggta aaaaaattga gagaacgttt
    32641 attgtttgag aaaaaaatat ttacaggtgt gagtggttcc aatattatcc gtttgttgcc
    32701 gcctctttgt ctgactatgc aggatgcaga acttttcctt gatcattttg ctgaagtgat
    32761 aaaataattt tgtaatttct cttagtttca tgaaaaaacc gatagccctt ttgtttatcc
    32821 ttttttttgt aaatagttgg actgttttct ctcagtctgt ttcaaaagaa cgggataaag
    32881 tcgtgatgaa aaccggtgaa atttattacg gagaagttgt ttttcgaaat gacgaaatgc
    32941 ttattttgca aacagacgaa ggagttcgat atcagttttc tattgcggaa gtgaaagcca
    33001 tttacaaaga aacggtagat ggttctgatg agttagaaaa aatgcaacag gaaggtcagt
    33061 ctgatgaaat acagaacttt tttgcagtgt tggatgcgtg tggaggaatc tcgagtgcca
    33121 aaaacagttt tgatgtttct tctaatactc aagtagcatt tgttttgggg aaagataagg
    33181 ttttttcgga aaattttttc gtgggtttgg gtgtcgggta caattcttct tatttaaaga
    33241 gtaattcgtc actgaatttt ctccccgttt ttcttcgctt gcaaaatact tttactaaca
    33301 atcggatttc gccttttgtt cgcatggatg caggatacgc tttttctatg acttccggct
    33361 gggatggagg tgcattggtt aaagcggctg ccggtatcag ttcnnnnnnn nnnnnnnnnn
    33421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33481 nnnnnnnnnn nnnnnnnnnn nnngcattaa atgaacaggc gtgtgcagaa aaagctcagc
    33541 atcacctccg gtttttcaaa actgctccgg gcgaatatgg cgaaggagac aaatttattg
    33601 gtgtgaatgt tcccgatcag cgaaaaattg ccaaaaaata tttcagagaa attaatttga
    33661 atgagctgga agttttatta acgaatgaaa ttcacgaatg ccgttctaca gctcttttta
    33721 tgctttgtta caaatttgac aaagccggtg aagcggaaag aactttgatg gcggacttat
    33781 acctcaataa cctatctttt gtaaataatt gggatctggt agatgcttct gctgcaaata
    33841 ttttgggttg ttacttgatg gacaaaaaaa gagacttgct ttacgacttt gttcaaagta
    33901 acgatctttg gaaacagcgc atagccatca tcgctacgtt ttattttatt cgttgtggtg
    33961 agtttgtgga cactttcaaa atttccgaaa tgctgcttca tcatcctcac gacttgattc
    34021 ataaagccgt tggttggatg cttagagaag tggggaacag agataaggaa gcagaaatca
    34081 actttttact aaagcattat cggcaaatgc ccagaaccat gcttcggtat gccatcgaaa
    34141 aatttgatga aggtttgcgc cggcaatttt taaaagggta aatttaccta taaattccta
    34201 ttgcattcgt tcaattttat taggtgtgat caaagatgct tttcctactt tttatagagt
    34261 cggactttat tttttcgtcg gggaaaaaag attatccaaa gtccggagat attattttaa
    34321 ttctggagcg ttatttaaaa aattatggag taattttgtg agttcaaatg aatgcttctt
    34381 tatggaagcg acttaaaaat ttcccgcaaa atgaaattaa aagaaattgc ccaaatactt
    34441 gggaattcac atttaaaagt tcctgaagtt gaaataaatt ggttattgac cgatagccga
    34501 gctctttcgt ttccaacaag aagtttgttt tttgctttgc gtacccggcg aaacgacgga
    34561 catcgttaca tacaggaact ttatgaccag catttaagat attttgtggt cagtgaaacg
    34621 cttccagaat ttgaaaaaat gcacgatgcc attttcattc gggtaagtga tacgctggaa
    34681 gctttgcagc atttggccgc agttcacaga aatgcttttc aaattccaat tgtgggaata
    34741 acggggagca atggtaaaac aattgtcaag gaatggttgt atcaactgct tcattccgat
    34801 ttcaacatta cccgatcgcc acgaagttac aattctcaaa tcggtgtgcc gctatcggtt
    34861 tggggattga acaacaatac cgagcttgga atttttgaag ccggtatttc tcaaccgcac
    34921 gaaatggaac gtttggaacc aattattcga ccgaccatcg gaatttttac caatctcggc
    34981 gatgcacatc aggagaattt ttccagcttg aaacaaaaat gcgaagaaaa gcttaaactt
    35041 tttgcccatg ctcaagtttt gatttattgt tcggataata gattggtgga tattacggtt
    35101 gctcagtcga atttcaaagg caaattattt tcttggggaa gaaatgaaaa tgctatttta
    35161 caacttgtta aaacaaaaaa actatccgat tcgacggaag tagtttataa attttcagga
    35221 aaagaatcgg ctgtttccat tcctttttcc gacgatgctt ctatagaaaa tgccatgcat
    35281 tgcgtagcat tgatgttaca tctcggaatc gattcggaaa aaattcgtca acgcatagcg
    35341 aaactcgaaa gtgtagaaat gcgccttgaa ttgaaggagg ggattcacgg atgccttttg
    35401 attaacgaca gttataattc cgatttgaat tcgttggcaa ttgcgttgga ttttctcaat
    35461 cagcaggctg caaccaaaaa tttgccgcga acgttgattc tttccgacat tttggaaagt
    35521 gggaaacagt cggatgattt atataatgcc gtagctcagt tggttaaaag taaaagcata
    35581 cagcggtttg ttggtatagg cccagaaatt gtcaaacatg ccgataaatt tgaggtagag
    35641 caacaaaatt ttttcgaatt tacggaagat tttttgaagt cgccacttat ccacgaattg
    35701 cagaaagaaa tcatattatt gaaaggttca cggaaatttc attttgaaaa aatttccgaa
    35761 aagttggaac tgattgctta cgaaaccaca ttggaagtaa atttgaatgc tttgatcaac
    35821 aacttcaact attttcgttc caaactccgt cccgaaacca aagttatgtc tatggtaaaa
    35881 gcttttgcct atgggagcgg atcggtagaa gttgcacgaa ccttgcaact ccatcattgc
    35941 gattatttgg ctgtagcagt agccgacgaa ggagccgaat tgcgccggga aggaattcgt
    36001 atcccgatag tggttatgaa tcccgaaaaa ggaagtttcg gaatgatttt cgagcatcaa
    36061 cttgagcctg aaatttacag tttcagaatt ttggaatcat ttctgtcggc agccgaaaaa
    36121 ttgggtgtaa ccgattatcc ggttcacata aaaattgaca gtggcatgca tcgattggga
    36181 tttgaacccc acgaaattgt tcctttgata caacggctga aagcacaaaa caacctgaaa
    36241 gtgcggtcgg tattttctca tttggccggt gccgacgaaa aacgtttcga tgattttacg
    36301 cggcaacagg taaaagtttt cactgaatgt gccgatttgt ttgctcagtc gtttgagcat
    36361 cccattataa ggcatattct caactctgcc ggcatagaac gttttcccga atttcaattt
    36421 gacatggtgc gtttgggaat cgggcactat ggtgtcagtg cgttgcccaa cgttaaactg
    36481 gaacctgttt gtgcactgaa atccattatt ttacagatta aaaatgtaaa aaaaggagaa
    36541 acggtcgggt atagtcgcaa aggaattgcc aacgaagaca aacgaattgc cgttattccg
    36601 gtgggttatg ccgatggctt cgacagaaaa ttgggaaatg gtgtcggcga agttttgatt
    36661 aacggaaaac gcgccaaagt gatgggtaat gtatgtatgg atttgacaat ggttgatatt
    36721 accggtattg aagcaaaaga gggagattcg gtggaaatat tcggcaacaa cctgtctgtc
    36781 atcgaagttg ccgacaaatt gaaaaccatt ccctacgaag tgctcaccag tgtttccaaa
    36841 cgagtgaaaa gagtttattt tcaagaataa attcagtctg ttttggtatt taagttgagt
    36901 tttatttaaa aaattagatg atccttattt ttgtacaaaa tacaaccccg ccggttgcac
    36961 ttctgcaaag tgttgccaca ttgtctgtcc aattgaaaaa ggaatggggc atactttgtt
    37021 tgacggaaaa tgccgatttg gtttcagttt acgaaaatca atatggagaa tttttaaaaa
    37081 caaacggatt tccggaagta tccatatttg tgagacaagc tcgttgtgag caacttgcag
    37141 aattttgtga acaactggaa gcttcttttc ttttttttca gttgcatgat gcatcccacc
    37201 gatatcttcg tcgggcatta aatttttgtc gtgatttgcg cataccgtat ttgtttttca
    37261 aaaattcctt ttcggtgctt aatttggaga aagttgtagt tccgataggt tttttggaag
    37321 aagaaataga aaaagcgcag tttgcatctg cttttgggcg atttagcaat gctgaaatca
    37381 ttttgctgcc tgccaacgat tatgggtcta aagctgcaac gacaaccgat aaaatgaaaa
    37441 ctatcctcga caagtttcaa ttgccctaca cggttgagaa agcacaatcg gacagtttca
    37501 aaatcgacaa agaagcaata gaaaaagcag ttgaaatggg agcagggtta gtcatcattt
    37561 ctgcttcgcg agattacggg ctggatgata ttctttttgg gcccaaagaa ttgcacttga
    37621 tacggaaatc tcccgtgccg cttttactgg ttaatccgcg aagcgatttg tatgcactct
    37681 gcgactgaga atagtttaca gtgtttattt caaaaaaagt tcgccaaaaa actccggacg
    37741 atggaaatcg ggtttttcgg tttttatggg attccaactt aaataatgtg gcgattctgt
    37801 tccatcagcg catttgtaaa aatttcccag aattttttcg ggtaaattat tcgggtcaat
    37861 gcctatcagt ttaaagggaa tttttactgt caactcccaa tcgaaatttc cttttatttc
    37921 ctcaaaaggc tgtggcacga tggaagggaa tctttcaatg gatttcattt ctgtttccga
    37981 aagaggttca acttgatcgt ttcggccttt acgccgggaa gccgagcaag tcccaatgca
    38041 attgaattca aaattaatat atttctcact ctcgatagtt ttgcagaaaa attctacgca
    38101 actgtcttcg taaaccggtt cctggtcgcg cgaataaacg gcacgcagcg cattgcacaa
    38161 aacaaagaat ttgataaata aagcttcttt cgactgcgca atgtggaaat aagcaatggg
    38221 tttgtaagga aactcttttg cccagttttg ggattcaatg gcggcttttg ttccgttttt
    38281 ttccagtatt acgctcgctg ttgttatgtc tgctttgtct aaatccggaa tataaccgat
    38341 atgcaatgat ttcatcagaa tataatttat tttttaaagg tctgatggaa cccacatgtc
    38401 tga
//
LOCUS       DAOSIB010000009        37870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_1248_length_37870_cov_10.596113, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000009 DAOSIB010000000
VERSION     DAOSIB010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 37870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37870
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1248_length_37870_cov_10.596113"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1127)
                     /locus_tag="PLD44_01725"
     CDS             complement(165..1127)
                     /locus_tag="PLD44_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOF98089.1"
                     /translation="MKLKYVVILLSLIFLSCRKGKIEDQETNKVLVPLADPYILLYDG
                     MYYAYGTGADNGIEVMTSSDLKTWKRRTNTTSGLALYKDDSYGEKWFWAPEVYYVNEK
                     FYMYYSADEHICVAISDSPLGPFRQVEQKPMLEEEKAIDNSLFVDDNGKAYLFFVRFN
                     DGNNIWVAELEDDLMTLKKETMHHCISVSQPWEEIWPRVNEGPFVIKHNGIYYMTYSA
                     NSYESPFYGIGCATATNIMGDWTKYPDNPLLQKPGNLVGVGHNSLFFDKEGNLRIVFH
                     AHHDINTIHPRNMYISNVGFKHVDGIDKMFINPNYLTPFLEVIR"
     gene            complement(1129..2706)
                     /locus_tag="PLD44_01730"
     CDS             complement(1129..2706)
                     /locus_tag="PLD44_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOF98090.1"
                     /translation="MKKYLYLLMIASTFLLNGCDVDVINPNTLTQATFWKTQRDAEYG
                     VNAIYNMFYKPGTYSRWIWFRLDLTSDEGWSQSPWAELKEWTRFQYVNYNFWEGNAWT
                     YRDCYEAIFRANQALENIPNIEMDETVKNQLLGQAYFLRGLYYYDLALLWGSTNNSLP
                     INLKPSKPADTPEGHTGEAVYNQAISDLTQAVNLLPEQWDSNNLGRATKGAAYALRAK
                     CYMQLHDWTKAKNDLYWLVEGDGKQYYDLVANYEDNFTKDTENNKESVFEIQYSDVHK
                     APAGDGDFDVDPNLGLNRGQFFAPPGIGWTDGELRPWIVEEFKKERDLNGNFDIRLKY
                     TAFYEGMNQDFTDNNRIYRYRLDGADANIWNLQNWKGRVFFRKYGSDYYRDYDDYHNP
                     TNVRFIRYADVLLMYAECIANTENDFSKAVDLVDRVRQRANMPKLSVNHLDATQSKEK
                     FLKRLQTERALELCSEGHRWADIKRWGLVDTQNGLNELIARDADFQNFVIGKHAVLPI
                     PSDDVNNNPNQQQNPNY"
     gene            complement(2733..5735)
                     /locus_tag="PLD44_01735"
     CDS             complement(2733..5735)
                     /locus_tag="PLD44_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOF98091.1"
                     /translation="MKNKFYLLLVLLSFPLLIFAQAQREVKGKILNENGEPVIGANIF
                     ESGNPTNGTISDLEGNFVIKISPQSAIEISYIGYVPQKIKDPKSPLTIQLEVDQRSLD
                     EVVVIGYQTVKKSDLTGAVSVFKPETMKNTVVTGTIGEALYSVPGIFVRSAGAPGNEG
                     FIQIRGTSTFGTSNPLYIIDGIDVGTANRDFNYNDIESIQVLKDASAAAIYGSRAANG
                     VIIITTKKGSKGKMKIDFSAKNTFQWLPQYNLTNREQWITLNDLAFKNAGRQPANHSD
                     GNTNWQDEVFKTGILQDYDLSFSGGGETNTYYLSANYQTNSGTTYGTNSERITARINT
                     SSFRDFGKNVRFTIGENLIISNYFVDELNTNPITDVWRMLPTIPVYDPSNPGGFGYGD
                     GSKDVTFGTNPIARENLENTTNENLRIRGNAFAELSFFKSLKYKFNLGLVTSSDSHMY
                     LRKVGNWTYNQPIDPSSLNRNKALYKFMVYDNTLEFNKTFGVNNIAAVAGTSFSTEDY
                     SQIWGTKNNVLTSGDDYFTQLDAALLDPKTGSYQNLAKLFSIFGRINYSYDDKYILSA
                     TVRRDASSKFGPNYRNGVFPSIAGAWRISKEKFFNVSWIDDLKIRANYGILGSSNIGY
                     WDWVPFINIFPQAIFGAGQNINTGMTQVKLVNTDLKWEELHQTNAGFDAYLLNRRLEV
                     VGDYFNKTTQDVLTPMQILLTTGNNGGNPMVNAATLKNTGFEVSVNWRDRINDLNYSV
                     GLSTSYLKNEIVELGYGRESFTQWDTKSFVGKPIGDWYLIKTDGIFRSTDEVLNHVNS
                     KGQVIQPSAKPGDIRFVDFNDDGQITDADRQYCGHSFPAWNMALNGTLEFKGFDLMFQ
                     FTSSFGKDFKLFNGPRSGYDRFDDNSNYRADYDGYDAVTNPNGVDPRPLYADARNARG
                     DQDRWLENGSYLRLKQVGIGYTFPKKAFNNVLSNFRLFVNAQNLFTITKYRGLDPEFL
                     NSNIWDRGYDSAPFPNPYGITVGTQISF"
     gene            6248..7333
                     /locus_tag="PLD44_01740"
     CDS             6248..7333
                     /locus_tag="PLD44_01740"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HOF98092.1"
                     /translation="MNFDISFTNKEITPWGGMIFLKQMLEKIGFRDLITSNPDLPVSG
                     SNRGYKTSTIIEGFITSVWCGANRFLHTEVTRHDAALGKIFGWKNTPGQDTYKRFFSK
                     FTQVTNQKVSDYFFSWIFDNVHFNHFILDVDSSVMTRYGEQQGAKKGYNPQKRGRASH
                     HPLIAFVADLKLVANMWLRSGDTSSANNFLSFLEDTLSKLKNKTIGLIRMDSGFFQTE
                     ILDYLEEKAMDYIVAVKFTRPIQRFISQSKNWIVLDKGIEISEQLYQSESWEKPRRIV
                     IVRQKLKDRPKAPGKQLRLFEEEEVYRDYRYSAYVTNMKLSAPEIWRIYRGRGDAENR
                     IKELKYDFGFDSFNLKNFFARKAVLIL"
     gene            complement(7665..9032)
                     /locus_tag="PLD44_01745"
     CDS             complement(7665..9032)
                     /locus_tag="PLD44_01745"
                     /inference="COORDINATES: protein motif:HMM:NF039698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6242 domain-containing protein"
                     /protein_id="HOF98093.1"
                     /translation="MLRNQLKILALLGTIFFLNSCLGDGNVKEISSDPSFVSLTFSAN
                     NKIPNLKDAVFSLQYDSVIVNLDSLPYQTRIDSVYATFTFKSTYAAYLVYENDSTALI
                     SGNEAFDFTKIKAIINIASDGVTRRKYLIKVNVHQVEPELYIWQKVNDAAHSLNVQHQ
                     KAVYFNDRLYLFVNDRQQNYLFVSPNGVSWTQDVISGLPTNATFEDITVFNNQLYVTC
                     GNSNIFSSGDGLTWTRQTYSEYDFTSLIFVLNDRLWAVSHSLTDGKLRFASSVDGTEW
                     LIYNEIPENFPVRGFASVVFNTKVNKQKGLVLTGFSSDGTALKTNWNTEDGIYWVDFS
                     AENASLDSIGRGASVINYDDKLLLFGQDNKPIPESYFRQSVDQGFSWQVPDSNLNNLP
                     KEMGIRHYSSVVVYRPRTYSSTDSKQEIESSNWIFIIGGKNAYDEYLTDVWKGKVNRK
                     NFLRQ"
     gene            complement(9150..9914)
                     /locus_tag="PLD44_01750"
     CDS             complement(9150..9914)
                     /locus_tag="PLD44_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOF98094.1"
                     /translation="MKIISYNVNGLRAALTKDFIQWLQAENPDVLCLQETKAQPDQID
                     TLLFAELGYTAYLHSAQKKGYSGVATFTKIPPDYVYFGMDNPEFDTEGRVLRTDFGDI
                     TVINVYVPSGSSGGERQDFKMKFLEEFLKFIQNLLRERPNLVVCGDYNICHKPIDINH
                     PERQIGVSGFLPEEREWMDRFQALGMVDSFRVFDQSPEKYSWWSYRGGARYRNAGWRI
                     DYHWVSEPLRSRLKAAAILSEAFHSDHCPVMVELDV"
     gene            complement(9955..10722)
                     /locus_tag="PLD44_01755"
     CDS             complement(9955..10722)
                     /locus_tag="PLD44_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF98095.1"
                     /translation="MIEVKNIIKTFDGVPALQDISAVFETGKVNMIIGQSGSGKTVLM
                     KCIIGLHQIDSGCIEYDGRNLPKMKPQEILMLRREVGMLFQGAALFDSMTVLENVMYP
                     LEMFAHESKEEIRERAYFCLRRVNLEERSYDLYPSEISGGMQKRVGIARAIALRPKYL
                     FCDEPNSGLDPLTSMIIDDLIYEITHEFNITTIINSHDMNSVMEIGEKIIYIEKGVKA
                     WEGTKDEIFDAENESFNNFVFASKLFKQLKMLKQNPY"
     gene            complement(10847..11587)
                     /locus_tag="PLD44_01760"
     CDS             complement(10847..11587)
                     /locus_tag="PLD44_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF98096.1"
                     /translation="MNLFQETGKYFLLMKRVFVQPDRSKMFFRQFPKELDKLGLQSLP
                     IVLIISVFIGAIMTIQTKLNTENPLLPTYSTGLVTRDTLLLEFSSTIMCLILAGKVGS
                     NIASEIGTMRITEQIDALEIMGVNSANYLILPKIVAFVVMMPFLVIFSMAVGLIGGYL
                     VGVFSDVITVADYLLGIQYAFNPFYVFYAMVKSLVFAFIISSVSSYYGYYAYGGALEV
                     GKASTNAVVNSSILILFFNLLITNVMLG"
     gene            12293..13813
                     /gene="purH"
                     /locus_tag="PLD44_01765"
     CDS             12293..13813
                     /gene="purH"
                     /locus_tag="PLD44_01765"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOF98097.1"
                     /translation="MEKKVIKKALISVYHKDNLEEIITKLHAAGVEFISTGGTKSFIE
                     SLDIPCEAVEDLTGYPSILGGRVKTLHPKIFGGILARRDNAQDVQQMSEYEISGIDLV
                     IVDLYPFEATVAAGADEPTTIEKIDIGGISLIRAAAKNFKDVVVIASKEQYEPFLEIL
                     NSSGAQTSIEERRWFAKEAFAVSSQYDTAIFNYFDEPYQSAFRWYENHPKVLRYGENP
                     HQRGIFFGKFSEMFEQLQGKEISYNNLLDIDAAVNLINEFDDITFAVLKHNNACGIAS
                     RPSLLEAWKAALAGDPVSAFGGILITNAIIDKETALEINKIFFEVIIAPDYDLEAIEI
                     LSQKKNRIILIMKNNTLRPKQFRSLLNGVLMQDRDLHTETAADLKVVTEKSPSEAEIE
                     DLLFANKIVKHSKSNAIVLAKNKQLCASGVGQTSRVDALVHAIDKARSFDFDLKGAVM
                     ASDAFFPFPDCVQIAHEAGITAVIQPGGSIKDQDSIDFCNQKGLSMVFTGYRHFKH"
     gene            13860..14879
                     /locus_tag="PLD44_01770"
     CDS             13860..14879
                     /locus_tag="PLD44_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOF98098.1"
                     /translation="MGFFSFTQEIAIDLGTANSIIVYNDQIVVDEPSVVALDKRTDRL
                     IAVGERARQMQGKENESIRTIRPLRDGVIADFYAAEMMIRGLIKMIPSKNRFFTPSLK
                     MVIGIPSGSTEVEMRAVRDSSEHAGGREIYMIYEPMAAALGIGIDVEAPDGCMVVDIG
                     GGTTEIAVISLGGIVLNKSIRIAGDDLTDDIVEYMGRMHNIKIGERTAELIKINVGAA
                     LTDLEDAPEDYIVRGPNRMTALPIEVPVSYQEIAHCLEKSISKIEAAILSALELTPPE
                     LYSDIVQHGIFLSGGGALLRGLDKRLTDKINIPFHIADDPLHAVARGTGIALKNVDKF
                     SFLLR"
     gene            14991..15836
                     /gene="mreC"
                     /locus_tag="PLD44_01775"
     CDS             14991..15836
                     /gene="mreC"
                     /locus_tag="PLD44_01775"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOF98099.1"
                     /translation="MKNLINFLIHHSGFFVFLLLEILSLMLVFNNRDYQKSVFFSSSN
                     TIISSFYNWNNYIVGFFNLSKSNQQLAEENTQLKNQLLDLQNKLEALQPSGTDSLSYK
                     IPPEKQYRFIEAKVINNSTNKLQNYITLNKGALDGVKPDMGVINDEGIVGIVKNVSQH
                     FSVVIPVLNPKIQINCKFKRNNYIGSLVWDGKDYRFASLTDIARHVPIKMGDTLVTSG
                     LTTTFPEGIPVGTIENFNLKESDAYYEIKVKLATNFRTLSYVKVIDFKNAGELKELEK
                     TAYEE"
     gene            15838..16338
                     /gene="mreD"
                     /locus_tag="PLD44_01780"
     CDS             15838..16338
                     /gene="mreD"
                     /locus_tag="PLD44_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOF98100.1"
                     /translation="MAITVFQNIIRFVLLMMVQIFVLNNIQLSGYINPYIYILFILSL
                     PYRTPQWLTLILAFVTGLMVDIFMNTMGTHAFATVLMAFTRNGIINLITDFEKDENPT
                     PSFYTFGIGVYIRYIVLLVFIHHTALFFLEAFSLAHFGIVIKKIFFSSVVSILLILGI
                     QSLKKK"
     gene            16342..18222
                     /locus_tag="PLD44_01785"
     CDS             16342..18222
                     /locus_tag="PLD44_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOF98101.1"
                     /translation="MINDSLQNRRYVIAGIISVVIALYIIRLFSIQVIETKYKQGAVS
                     NAFLNKTLYPPRGLIYDRNQNLLVYNKPAYDIAVIMREIHDLDTLAFCRALNIDQTFF
                     NKRMAEIKDRKKNLGYSAYTPQLFLTQLDIQDIATIQQMMYKFPGFYIQSRTLREYAY
                     PYAAHVLGSIGEISQSRLDEDNYYVRGDYVGRDGLEYTYEKDLRGEKGVEVLLRDAKG
                     RIKGKYENGKHDVASKTGKNLITTLDIQLQMLGEQLLNGKIGGIVAIEPKTGEILAMV
                     SSPTFNPSSLVGRQRTKNYIKLANDPAKPLLNRATQAQYSPGSTFKTIQALVCQQMGG
                     IDTKTLFPCNGPASSPIKCTHHHGSPVNLLNAIEQSCNPYFWGAFRTTLEKDGYGEKN
                     KNFKRNYQIWRSYVLSFGFGKKFTDGDIYQQSSGNIPTEKYFNRYFGETGWRAMTIRS
                     LAIGQGEILVTPLQLSNAMAIIANKGYFVTPHLNKADSLLNRVHRTSVEKNYFDIVDE
                     GMWRVCEFGTARHYKVPNLSMCGKTGTVQNSRGKDHSIFVGYAPRENPQIAIAVVVEN
                     AGFGATWALPIASLMIERYINGKIERTDLLERIASATLSASATRLTNKTNLTDFINGQ
                     DF"
     gene            18206..19654
                     /gene="rodA"
                     /locus_tag="PLD44_01790"
     CDS             18206..19654
                     /gene="rodA"
                     /locus_tag="PLD44_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOF98102.1"
                     /translation="MARTSNIKYDWITIIYYVLLVFMGWLSIYGASYDFEHPAIFDFS
                     QRAGKQFIWILTAFGIAGILLLIDDRIYNYFSYFIYGFTILLLIATIFVAQDIKGSRS
                     WIVIGPISFQPAEIAKMSTSLALAKFMNTYGFKLRSWKNLIPLLVIVFIPLILIMLQN
                     ETGSALVFLSFFLMFYREGMNGIVLLIGALAAFFFVVVIRFSIVPIAFGTGTLGIVLA
                     MTLIIVGQFIYAYFFVKNKKEALYLLGGSSLVALVAYLLSLKFKVNFNSVAFFTVLAS
                     CVYWMVMELKFRHKKFLYLSLISLGFSLFSFSSDYIFEKTLEPHQQTRIKVLLNMEND
                     PMGAGYNINQSKIAIGSGGFWGKGFLNGTQTKLKYVPEQDTDFIFCTVGEEHGFFGST
                     IVLILYWLFLMRLISIAERQRETFNRVYAYCVVSIFFFHIMVNIGMVLGIMPVIGIPL
                     PFFSYGGSSLWGFTILLFILLRLDASRFEHIH"
     gene            19756..20496
                     /locus_tag="PLD44_01795"
     CDS             19756..20496
                     /locus_tag="PLD44_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF98103.1"
                     /translation="MKKLIIISLPLILFLFSMKASSQKFEKDVFSTSAGNLTITFIGH
                     GTLMFEIDNKVIHIDPTAMFADYSTLPKADLILITHEHADHFDMATIDKISKKETEII
                     LTTTCKNQLQKGKVMKNGDSYNWKKFIHIQAVPAYNIQHKTNTGQLFHPKGQGNGYIL
                     TFGNFKVYVAGDTENIPEMKSLKNIDVAFLPMNLPYTMTPKMTADAALSFMPKILYPY
                     HYGNTDTNELVNLLKNRPEIEVRIRKLN"
     gene            20564..21259
                     /locus_tag="PLD44_01800"
     CDS             20564..21259
                     /locus_tag="PLD44_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275865.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOF98104.1"
                     /translation="MKNRIVEAAIIAVGIICLGLCIRNGIKIFSQKDRAVEVKGLAEM
                     EVPANKVICPILYKDLGNDLAQLYVDMNKKNQAIISFIKENGIQENEISLSAPEIVDM
                     AAERFVENKSPYRYNLTSVITISTPNVDKVLALMSRQAELLKKGIAVTVNNYSVRYEY
                     TKLNEIKPQMIEEATKNARKAAEKFAKDSGSKLGKIKSATQGQFIISDRDENTPYIKN
                     VRVVSTITYYLED"
     gene            complement(21573..22247)
                     /locus_tag="PLD44_01805"
     CDS             complement(21573..22247)
                     /locus_tag="PLD44_01805"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOF98105.1"
                     /translation="MKSLTLSFIALFGAMVMAFGQFQLPKLDYGYSDLEPYIDSTTMY
                     IHYNNHHATYANNLNKTLEKYPELYKKDIVELLENINELPADIQLSVRNNGGGYYNHC
                     LFWSMLAPAGSTTISPKLEKILIKNFGSIDNFKNEFEKAALGQFASGWAWLIKESSGK
                     LRIISTSNQDNTLMPFSSVKGKPVLTLDVWEHAYYLKYQSKRASYVKAFWNVVNWKEV
                     EKLIEK"
     gene            22517..23107
                     /gene="ruvA"
                     /locus_tag="PLD44_01810"
     CDS             22517..23107
                     /gene="ruvA"
                     /locus_tag="PLD44_01810"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOF98106.1"
                     /translation="MIDYIKGQIVELTPTYVVLEANGLGYFISIPLPAYTLLSHQLEA
                     KLFVHEVVREDAYLFFGFITKKEREVFQLLISVSGIGVNTARMIMSSYSVEEIQEIIA
                     AGNVSALNAIKGIGSKTAQRIIVDLKDKILKGKDDGGSLSLLSDKNAQIKEEAVSALV
                     MLGFPVGASQKAIENILKEQSQLEVEQLIKMALKIL"
     gene            23187..30620
                     /gene="sprA"
                     /locus_tag="PLD44_01815"
     CDS             23187..30620
                     /gene="sprA"
                     /locus_tag="PLD44_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOF98107.1"
                     /translation="MTNRKKSSSIFVYFGIFLSSTVGFVYASLQQPVPAETRSNALKQ
                     QDSVQQHFPVKKLVQDTYEDITATYPLDAPVPSNIKTEIEYDPSTASYILRTYLGNIE
                     IATPFRMTEQEYLDYSGRQEMQRYWYEKNSSAQQNVEDKFSVTDMKFDIGPADKIFGP
                     GGVQIRTQGSAEISMGFKTNKIDNPALTERMRKSTILDFDEKIQMNVNGKVGDKVNFN
                     MNYNTESSFDFDQKLLKLNYKGKEDDIIKNIEIGNVSMPLNSSLITGSTALFGVKTDL
                     QFGKLSISAVASQQQSQTQTVSSKRGAQTTDYEIGIDDYDENRHFFLGHYFRNNFENA
                     MSKLPFVTSGVTITRVEVWVTNKRGNYDQARNIVAFMDLGETQRLDNHYWNVLSSTSL
                     PQNKANSLYDEVTSIPNVRDIRQTNNVLSSQYAADSIFGGEDYEKIESARRLDPSEYT
                     LNSSLGYISLRTALNPDEVLAVAYEYTYGGQVYQVGEFSTDAVTAPNALIVKLLKSTA
                     QSPSLATWDLMMKNVYNLGALQIQPENFQLDIVYRNDSVGTDLKYITEGNIQNQLLLR
                     VMNLDRLNTKNAAQPDGKFDYVEGYTVLSSSGRIIFPVLEPFGSHLRKAIGNDQIAEK
                     YVFQELYDSTLVAAREYSEKNKFRIVGEYKASSGNEIRLNAMNIPRGSVTVTAGGVTL
                     VENVDYTVDYTMGTVNIINTSILESGSNIDVKLENQSMFSMQRKSLVGTHLEYQFNRD
                     FSLGATVMHLSEMPLTTKVNTGDEPISNTIWGLNTSWRKESQWLTNQLDKLPFVNATK
                     PSTLALNAEFAQLIPGHSNVISSAGLAYIDDFESTKTTIDLHYPVFWHLGSTPFDPNG
                     GLFPEAALSNNVDYGKNRALLAWYYVDPILNANSRNTPENLRNNLESQSNHLTRNVLE
                     EEIFPNKERLATQTSRMTIMNLSFYPNERGPYNLDWEGINEDGSLKNPEKRWGGIMRK
                     LDVTDFENSNIEYIEFWMMDPFITDKDGTQKGGDLYINLGDISEDILKDGKKFFEQGL
                     SIDGDVTKSDTTVWGRVPKTQSTVKAFDNTPGARKYQDVGLNGLSTDEEFEYPTYKKY
                     VEQITRKISPQTLQKWQDDQFSPLNDPAGDNYHFYRGSDYDEQKKDILSRYKHYNGTE
                     GNSPEAENVNESYTTSATSLPDMEDLNEDNTMNEYEKYYQYRVQIRRDAFQVGKNYIA
                     DKITSNVKLANGNVEPVTWYQFRIPIREYNDKIGSIRNFKSIRFIRMFLTDFEKDIHL
                     RFATFDLVRGEWRSYTKSLYPNGLTPPSEGKLEVQAVNIEENADRTPVNYVLPPGVTR
                     ETDPGQPQLLQLNEQSMVLRVRDLAPNDAKAVYKTTSYDMRQYKRLQMFVHAEKLIDD
                     ISNLQDNELTCFIRIGTDMVNNYYEYEIPLRLTPHGKYLGENTEQREIVWYPENMFDF
                     AFSKLINVKLQRNKTLQASAGNVSNLVPYTIFDPDKPKNRITVVGNPTISDVQNIMIG
                     VRNRSGSIKSGEIWVNELRMSEFDESGGWAAMGNLAVGLSDIGTLNLSGRIETSGYGS
                     LESNVMNRNLEDLRQMNFAAAIELGRFLPERAKLQIPTYFSYSNETVSPKYNPLDQDV
                     ELSDALDNLPTKLDRDSLMNLSQTVSTSKSFNVANAKLNIKSKKPQFYDPANLKFTYA
                     YIENNEHNSEIEQNLIKNQRAAVDYNFNFAAQPWEPFKDVKALNSPAFKIIKDFNLYY
                     LPSSLNYNANLNRSYSQIKLRSFDVYSGSYTNDELSNLTFSKDFMFNRQFDIKYDLTR
                     SLHFSFQTVMNANIDEPYFTPEMGKEYYEAWRDTVWHNIKKLGTPYVYQQVFTASWNV
                     PLNKIPITNWMQANVSYNSNYNWNRSAQMEGGSNIGNTATSTGSWQFDGQLNFETLYN
                     KSKFLKNVLNRNKVTSARPQPFKPGNYTQTVQLKSDQPLTIQHRLGSERIRLTAKDKD
                     GNSVLLSYKVKNASSVEILPKQNMDSITINIVSLDPNQQSVVNKTRDITLGALMMVRR
                     FSINYRENNSTVLSGFMPETGFMGQQNYNGIDAPGYTFAFGFQEKDFIKKAFERGWLN
                     AGDSVVNPATTAFTSDLDIKATLEPIPGFKIDLNGRRYTASNTSVFYMYDGMPTTYSG
                     SFNITQIGLSTAFKKLGNATDNYNSETFNAFLANRQKVADRLNQKYIGTRYPQSGFIK
                     SSNLAGKEYDPSLGAYQLNSADVLIPAFLAAYTGRDATKISTNPFLSISSILPNWRIS
                     YDGLSKIEWFKNHFRNVNLTHAYSFRYSIGSYSSYSTWVPMEDESSLGYVRDVQTDNP
                     IPSSAYDFTSVSLNEQFSPLLGINATMNNSLSAKIEYRKQRNLALNLSSTQLIESASD
                     EYVLGFGYLLKDFDVILRLKSDKQSKIKNDLKLSADISYKDIKTLLRKVEENITQASS
                     GNKMITIKFLADYVFSSKVNIQLYYDHQSSTPLVSSSFPVSSSNFGASLRFMLTR"
     gene            30733..32733
                     /locus_tag="PLD44_01820"
     CDS             30733..32733
                     /locus_tag="PLD44_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOF98108.1"
                     /translation="MKKRFFLVLCCFFGFLFSEAQIFEPIHWDISHKLTGKTTADIIF
                     KASIDEGWHLYGLSLPPNGPKPTAIVFEKIENARKVGELQAVSNLEKKFDPNFGMELN
                     WFTKEAVFIQKITFSSKQNVRLEGYVEYMACNDKSCLPPTSEPFSIRLSTTEFNSINS
                     GEQAPSSESEKIQPTEDFKNAKQVDLSSEDTALTSSEWWTPVVKELNSFGNVVYHKET
                     TSLWYILLAGLLGGFLALLTPCVWPIIPMTVSFFLKRSAVPSRGRKDAVLYGLSIIVI
                     YVALGLLITLIFGASALNSMSTNAIFNLFFFVLLVVFALSFFGAFELTLPSSWSTKVD
                     AKSESTVGLLSILLMAFTLVLVSFSCTGPIIGTLLVEVASTGSLLAPAVGMFGFAFAL
                     AVPFTLFAFFPSWLESLPRSGGWLNRVKVVLAFIELAFALKFLSVADLAYGWHILDRE
                     VFVSLWIIIFALLGIYLLGKIRFPHDSESKHTSVGGLMLGIISLAFAVYMVPGLWGAP
                     LKSISAFAPPLYTQDFNLYKDEVHPAFTDFEEGMAYAAMKKLPVIIDFSGYGCVNCRK
                     MEASVWTDPQVKALLDKNFVLISLFVDDKTPLEKPFEINENGKTRKIKTVGDKWSYLQ
                     RYKFGANAQPFYVMIDYHGKPLNKPYVFDTNPSHFVNWLNRK"
     gene            33000..33581
                     /locus_tag="PLD44_01825"
     CDS             33000..33581
                     /locus_tag="PLD44_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOF98109.1"
                     /translation="MEKENKASEKNQSAENEKKHQQQHKENEDNIIPQSENSEAENTE
                     KELERLREECEDLADKNLRLLAEFDNYRKRMLKEKADLLKNGGENVLVNILPLVDDFE
                     RALKAIETSDDIEAVKEGIHLIYGKFVDFLNKNNVKEIPTENVEFNTQYHEAVSKIPA
                     PSEELKGKVIECLSKGYTLNDKVIRYAKVVVGE"
     gene            33655..34806
                     /gene="dnaJ"
                     /locus_tag="PLD44_01830"
     CDS             33655..34806
                     /gene="dnaJ"
                     /locus_tag="PLD44_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOF98110.1"
                     /translation="MAKRDYYEILGVSRTATEVEIKKAYRQKAIQFHPDKNPGDKKAE
                     ENFKEAAEAYEVLSDPEKRERYDRYGHAGVGSSASSGSYGGGMTMEDIFSHFGDIFGG
                     HFGGFSGFGGGSRSSAPRMRRGTDLRVKVKLTLAEIAEGVEKKIKVKKYVPCSHCQGT
                     GAAEGSAPVTCSTCHATGRVTRVQQTILGQMQTTSDCPTCHGEGKVIKEKCIHCNGEG
                     IIHDEEIVTINIPAGVAEGMQLTLSGKGNAARRGGINGDLIVVIEEEPHSELIRDGND
                     LIYNLLLSVPQAALGGAVEVPTVNGKVKVNIDAGTQPGKVLRLRGKGLPDVNRYGNGD
                     LLINIGVYIPEKLSKEEKTIMEKLEQSSNFKPSSSAQKGFFSRFKDMFD"
     gene            complement(35034..>37870)
                     /locus_tag="PLD44_01835"
     CDS             complement(35034..>37870)
                     /locus_tag="PLD44_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOF98111.1"
                     /translation="FVFSFVFPQFTKAVPAYPFPVKVVQPDGTEISVKLNGDEFFHYQ
                     TTLDGYLITRDKDGFFKYGKLSKKKEIAPSKYRVNNIEKRTKEEINFIKQLPKGIDFS
                     KQNQLNRIQRASSVTVTEKSGRKAYPLTGSPKSLVILVNFSDNSFTVSSPKEAFTKLL
                     NQNGYSENSSTGSARDYFYDTSTGKFNPQFDVVGPYTLPQTMGYYGENGSDGMDKNPQ
                     QMIIDACRLADNDDVDFSQYDTDRDGYVDNIFVYYAGYNEAEGAPANTIWPHRWTLAN
                     YNTKFDGVVVFDYACSSELRSSRGANMCGIGTFCHEFGHVLGLADYYPTDGGTHFTLS
                     YWNIMDAGAYLNDGKTPPTYSAFDRFFLNWLTPIELKSSGNFTLQPLTSSNSAYLISK
                     DGNHNLVGNSPNPSEFFMLENRQKSGWDAYLPGHGLLVTHINFNQSDWENNTPNNIAG
                     KLGYKIVEADGIGSEQTLAGDPFPGINNITTCSLVLRNGTKLKKSLTNITENNYINLD
                     FMGEENSPIVGAMSNFSAFETVQGTPSNAQLLKISGKKLKENILLAFSENKHFEMQLE
                     NDFNAEWTKNLILAPIDSVVDTTRILVRYNPTEPSYLDVHNEIISITSKDATPIYLNL
                     QGKSTRPVYVIPPVATEATDVTISSYVAHWEPVTDATGYYFTAYYISNESSETTEGFD
                     NGLTPSSGWTITAQALSTSASYSGKEVPAIQFKNSGEMIQTENYVLPAIKLSFFVKSF
                     SGNGALKVEAKNDSDKWITIDNVSVTTSLSAIKTYTFTESDHYNQFRLTYQKESGYLI
                     IDDITVGFAKKINYNEKDKWITSTTDTVINLPSNSDCYYKVKASDKTLRNSVILYENI
                     TDFSNLIYVHTLPDVGKQLKAYVNREDKTVTITVPNTSTVVRIYDVTGKLIVSEIPRY
                     NKFTIDALKNRIPGQLYIIVAGDRTAKIIM"
BASE COUNT        11800 a         7072 c         7326 g        11672 t
ORIGIN      
        1 gatggttctt tgttcttgaa ccctccttta gtgcctatta taagcttcta tagaataaaa
       61 gactgaaatt tatagaggaa atttatcata aaaaagcgtt ggagcaattt aatgacgaat
      121 aaatcgagca aatcctccaa cgcatttatg aattcaaatc aaaattagcg aattacttct
      181 aaaaatggag tgaggtaatt tggattaata aacattttat ctatcccgtc cacatgctta
      241 aaacctacat tgctgatata catgtttcgc ggatggatag tatttatatc gtgatgagca
      301 tgaaacacaa ttctcaagtt gccttctttg tcgaaaaaaa gcgaattgtg tccaacacca
      361 accaaatttc ccggcttttg caacaaagga ttatcggggt acttggtcca atcacccata
      421 atatttgttg cagtagcaca accaattcca taaaaagggc tttcgtaact attggccgaa
      481 taagtcatgt aatagatacc attgtgtttg ataacaaacg gaccttcgtt tactcgtggc
      541 caaatttctt cccacggttg tgaaacactg atgcaatgat gcatggtttc ctttttcaaa
      601 gtcatcaaat catcttccaa ttcagccacc caaatattgt ttccatcatt gaagcgaaca
      661 aaaaacaagt aggcttttcc attatcatcc acaaataagg aattgtcaat ggctttctcc
      721 tcttcaagca tgggtttctg ttcgacttgt cgaaatgggc ccaaaggaga atcggagata
      781 gcaacgcaga tatgttcatc agccgaatag tacatgtaaa atttttcatt cacataatac
      841 acttccggtg cccaaaacca tttctcgccg taggaatcat ccttgtaaag tgcaagtcca
      901 cttgttgtat ttgttctccg tttccatgtc ttgaggtcgg acgaagtcat cacttcaata
      961 ccattgtctg cgccggtacc atatgcataa tacattccat cgtaaagcaa aatatacgga
     1021 tcggccagag gaaccaggac cttatttgtt tcctgatctt caatttttcc ttttctgcac
     1081 gacagaaaaa tcaaactcaa caatataaca acatacttta acttcatttt aataattggg
     1141 attttgttgt tgattaggat tgttgttcac atcatccgaa ggaattggca acacagcatg
     1201 ttttccaata acaaaatttt ggaaatctgc atcacgcgca atcagctcgt ttaaaccatt
     1261 ctgagtatct accaatcccc aacgttttat atctgcccaa cgatgacctt ctgaacacaa
     1321 ttctaaagca cgctcggttt gtaatctttt caaaaatttt tcttttgatt gtgtcgcgtc
     1381 aagatgatta acagacagtt taggcatatt tgctctttga cgaaccctgt caaccaagtc
     1441 aacagctttt gaaaaatcat tttcagtatt ggcgatacat tcggcataca taagcaatac
     1501 atcggcataa cgaatgaacc ttacattggt cggattgtga taatcatcat aatcgcgata
     1561 ataatcagaa ccatattttc taaaaaatac tcgtcctttc cagttttgta aattccaaat
     1621 attagcatca gcaccatcaa gcctataacg ataaattctg ttattatcgg taaaatcttg
     1681 attcatacct tcatagaaag ctgtgtattt taaacgaatg tcgaagttcc cattcaaatc
     1741 tctttctttc ttaaattctt ccactatcca tggccgtaat tctccgtctg tccatccaat
     1801 tccgggagga gcaaaaaact gtccacgatt caatcctaaa ttaggatcga catcaaaatc
     1861 accatcaccg gcaggggctt tgtgaacgtc ggagtattgt atttcaaaca cggattcttt
     1921 attgttttcg gtatcctttg taaagttgtc ttcataatta gctaccaaat cataatattg
     1981 tttgccgtct ccttcaacca accagtataa atcatttttt gcttttgtcc aatcatgcaa
     2041 ttgcatataa cattttgctc ttaaggcata agcagctcct tttgtagcac gtcccaaatt
     2101 attgctgtcc cattgttccg gcaataaatt taccgcctgt gttaaatcgc tgattgcctg
     2161 attgtaaaca gcttctcctg tatgtccctc aggagtatca gcaggtttgg atggttttaa
     2221 gttaatcgga agactattgt tggtacttcc ccaaagtaaa gccaaatcat aataatacaa
     2281 acctcttaaa aaataagcct gtcctaaaag ctgattttta actgtttcat ccatttcaat
     2341 attcgggata ttttccaaag cctgattagc tctgaaaatt gcttcgtagc aatctctgta
     2401 agtccatgca ttcccctccc agaaattgta atttacatat tgaaaacgtg tccattcttt
     2461 aagctctgcc cagggacttt gactccatcc ttcgtcggaa gtcaaatcca gtcggaacca
     2521 tatccaacgg ctgtaagtgc ctggtttata aaacatgtta taaattgcat tgacaccata
     2581 ttccgcatca cgttgcgttt tccaaaatgt cgcctgagta agtgtattgg gatttataac
     2641 atctacatca catccattta aaaggaaagt tgatgctatc atcaataaat aaagatattt
     2701 tttcattgtg ttctaattta aatggtcaat tattaaaaag aaatttgtgt tccaactgtt
     2761 attccataag gatttgggaa aggtgcacta tcataacctc tgtcccagat attggaattg
     2821 agaaattctg ggtctaaccc gcgatatttg gtaatggtga aaagattttg agcattgaca
     2881 aacaatctaa aattactaag aacattattg aaagcctttt ttggaaaagt atatccaatt
     2941 ccgacttgtt tcaagcgtag atagctgcca ttttccaacc accgatcctg atcaccacga
     3001 gcatttctgg catcggcgta aagaggtcgt gggtccaccc cgtttggatt ggtaaccgca
     3061 tcataaccat cataatcggc tctgtaattt gaattgtcat caaacctgtc ataaccactt
     3121 cttggtccat tgaacagttt gaaatctttt ccgaaagaac tggtaaactg aaacattaaa
     3181 tcaaaacctt tgaattcgag cgttccgttt aaagccatat tccaagctgg gaacgaatgt
     3241 ccgcagtatt gacggtcggc atccgtaatc tgaccatcat cattaaaatc aacaaaacga
     3301 atatctccgg gcttagcaga cggctgaatc acttgtccct tactattaac atggttcaac
     3361 acttcatctg tagatctgaa aattccgtcc gttttgataa gataccaatc accaatcggt
     3421 ttcccgacaa aagatttggt atcccattgt gtaaagcttt cacgaccata tccaagttct
     3481 acaatctcat tttttaagta tgaagtactc aaaccaacag aataatttaa atcatttatg
     3541 cgatctctcc aattaaccga aacttcaaaa ccggtatttt ttaaagtggc ggcatttacc
     3601 attggattac ctccattatt acccgttgtc aacaatatct gcatgggagt caaaacatcc
     3661 tgagtggttt tgttaaaata atcacccaca acttccaaac gacgattcaa caaataagca
     3721 tcgaatcctg catttgtttg atgtagttct tcccatttca aatcagtatt gaccaattta
     3781 acctgagtca tacctgtatt aatattttgg cctgcaccaa aaattgcctg aggaaatata
     3841 ttgataaatg gaacccaatc ccaataacct atattggaac ttcccaaaat accataattt
     3901 gctctgattt ttaaatcatc aatccatgac acattgaaaa atttttcctt actaattctc
     3961 catgctccgg ctattgatgg gaaaactcca tttctgtaat ttggaccaaa ctttgaagag
     4021 gcatcccgac gaaccgttgc actgaggatg tatttatcat cataactata gtttattcgt
     4081 ccaaaaattg agaataattt tgccaaattt tgataacttc ctgtttttgg atccaacaaa
     4141 gcagcatcca attgtgtgaa atagtcatct cctgaagtta gcacattatt ttttgttccc
     4201 caaatttgag aataatcttc agtggaaaaa cttgtacctg ctacagcagc aatgttattt
     4261 acgccgaaag tcttattaaa ttccagtgtg ttatcgtaca ccataaattt gtataaagct
     4321 ttatttctgt tcaaactgga aggatctatt ggttgattat acgtccaatt accaactttg
     4381 cgtaaataca tgtgagaatc tgaactggta accaagccaa gattaaactt atatttcaat
     4441 gatttaaaga aacttaattc agcaaatgcg ttacctctga ttcttaaatt ttcgttggta
     4501 gtattttcca aattttcacg agcaattgga ttagtaccaa aagtaacatc tttggaccca
     4561 tctccataac cgaaccctcc gggattagat ggatcataaa ccggtatggt tggcaacatt
     4621 ctccaaacat ctgtaatcgg gttggtattc aattcatcta caaagtaatt gctgataatt
     4681 aaattttcac caattgtaaa tcttacattc ttcccgaagt cgcgaaaaga agaggtattt
     4741 atacgggcag ttattctttc actgttggtt ccgtaagtag ttccactatt ggtttggtag
     4801 ttggcagata agtaataggt attggtttcc ccaccacccg aaaaactcaa atcataatcc
     4861 tgtaaaatac cggttttgaa aacttcatcc tgccaattgg tattaccatc gctatggttg
     4921 gctggctgcc ttccggcatt cttgaaagca agatcattca aagttatcca ctgttcacga
     4981 ttggttaaat tatactgagg taaccattga aaagtgtttt ttgctgaaaa atcgattttc
     5041 atctttcctt tgctaccctt cttggtggta ataataataa ctccattagc tgcccttgac
     5101 ccataaatag cagctgctga agcatctttc aatacttgaa tgctttcgat atcattatag
     5161 ttgaagtctc ggttagctgt tccaacatcg attccatcta taatatataa aggattactt
     5221 gtgccaaaag ttgaagttcc cctgatttga ataaaacctt catttcctgg agcaccggct
     5281 gatcgaacaa aaatacccgg aacgctatat aaagcttcac cgattgtacc ggtaacaacc
     5341 gtgtttttca ttgtttcggg cttaaaaacc gaaacagcac cggtcaaatc cgattttttc
     5401 acggtctgat aacctatgac tacgacttca tccaaagaac gttggtccac ttctaactga
     5461 atggtaagag gtgattttgg atctttaatt ttttgtggca cataaccaat atacgatatc
     5521 tcgatagccg attgcggaga tatctttatt acaaaattgc cttccaaatc agaaatagtc
     5581 ccattcgttg gattacccga ttcaaaaata ttggccccta tgacaggttc tccattttcg
     5641 ttcaaaattt ttcctttgac ttctctttgc gcttgagcaa atattaaaag cggaaaggat
     5701 aaaagaacta gcaataaata aaatttgttt ttcatagatt taatttttta aattgataaa
     5761 tttcacaaaa atttgctcca taaaagtaga tactaaaata aattcaacat agaatttttg
     5821 tatcaaaaaa taaaaaaata ttatcaatta ttcgattttt taaacttatt agtttgccag
     5881 ctacaggtat caaaattcga aggttatgca attttctctt ttttaaagaa tgatttctac
     5941 tcaacaattt aatactcata tcctaaattc ccgatgtatt gctctttctt aaattatttg
     6001 ctaatgcata aattttcttg tcttcaaaag agctgaattt gacctgatga tgttatctct
     6061 ttgtttgtaa agtgtaaagt atatattcaa gtttgtaact cgctgtgttt tattgctact
     6121 cattttcttt taaaactttt ttctcggtta aagatttgtg ggtgagttta acccaaaatc
     6181 agcaataggg aaaaaagttt taattttgta ggaaactcta atgcgtagca atagaaaaaa
     6241 ggcgtgtatg aactttgata tatcctttac aaacaaagag ataacaccat ggggtggaat
     6301 gatttttttg aaacaaatgt tggaaaaaat cggttttcgt gatttgataa caagtaatcc
     6361 tgatttaccg gtttcaggtt caaatcgggg ttataaaaca tccacaatta ttgaaggatt
     6421 catcaccagt gtatggtgtg gagccaatcg atttttacat acggaagtta cccgacacga
     6481 tgcagcactg gggaagattt ttggttggaa aaatacgccg ggacaagaca cctacaaacg
     6541 tttcttctcc aagttcacac aggttaccaa tcaaaaagta agcgattact ttttttcatg
     6601 gattttcgac aatgttcatt tcaatcattt tattttggat gttgattctt cagtaatgac
     6661 ccgctatggg gaacaacaag gtgcaaaaaa aggctataat cctcaaaaga gaggcagagc
     6721 aagtcatcat ccgcttatag catttgtagc agatttaaaa ctggtagcta acatgtggtt
     6781 gcgaagcgga gacacttctt ccgccaataa ttttctttca tttttggaag atactctttc
     6841 aaaattaaaa aataaaacca ttggattgat tcgtatggat agtggttttt ttcaaaccga
     6901 gatattggat tatctggaag agaaggcaat ggattatata gtagcagtaa agtttacccg
     6961 acctatacaa cggtttattt ctcaaagcaa aaattggatc gtcctggata agggaataga
     7021 aattagtgaa caattatacc aaagcgaatc atgggaaaaa ccgcgtagaa tcgttattgt
     7081 tcgtcaaaaa ttaaaagaca ggccaaaagc accggggaag caacttcgtc tgtttgagga
     7141 agaagaagtt tacagagatt atcgttattc tgcttacgta accaacatga aactttcagc
     7201 accagagata tggagaatat acagaggaag aggtgatgct gaaaacagaa taaaggaatt
     7261 aaaatatgac tttggctttg acagttttaa tctaaaaaac ttttttgcaa ggaaagccgt
     7321 tttaattttg tgatgatagc ctataatctt atgtcgctgt ttagaatgtt cattttgcag
     7381 gaaaaaacgc agaaaacttt atctacactt cgatatagaa cttttgccat cggtgcatat
     7441 tttgaaaaaa tcaacaacaa actggttctc aaaatcgctt taaataaaaa aaggcgagcc
     7501 tggttttccg gcctatggaa cagttcttct gagtttgact ttccttttaa attggctaat
     7561 gagtaatctg ggtttaactt ttttctatat tatgggctgc caattaataa aaaaagcgcc
     7621 ttttatgaag gcgcttagta agtaattagc ttatagttaa acttttattg tcgtaaaaag
     7681 ttttttcggt ttactttacc tttccaaacg tcggtgagat attcatcata agcattcttt
     7741 ccgccaataa taaatatcca gttagaagat tcgatttcct gctttgagtc agtagagctg
     7801 taagtacgtg gccgataaac tacgactgaa gaataatgac gtattcccat ttctttgggg
     7861 agattattga gattactgtc tgggacttgc caactgaaac cctgatcgac agattgacgg
     7921 aaataacttt ctggaattgg tttgttatcc tgtccgaaaa gtaagagttt atcatcgtaa
     7981 tttataaccg aagctcccct ccctatagaa tcaagcgaag cattctcggc gctgaagtca
     8041 acccagtaaa tgccatcttc tgtgttccaa tttgttttca aagcagttcc atcggaagaa
     8101 aaaccggtca acaccagtcc tttctgcttg ttcactttcg tattgaatac cactgaggca
     8161 aatccacgaa ccggaaaatt ttcaggaatt tcattgtaaa tcagccattc tgtaccatcg
     8221 acagaagaag caaaccgaag ttttccatct gtcaaagaat gggaaacggc ccaaagccga
     8281 tcgtttaaaa caaaaatcag tgaagtaaaa tcgtattcgg aataagtctg tcttgtccat
     8341 gtcaatccat cacctgaaga aaaaatgttc gaattcccgc atgttacata aagctgattg
     8401 ttgaaaactg taatatcctc aaaagttgca tttgtaggaa gacccgaaat aacatcttga
     8461 gtccatgata ccccattggg agaaacaaac aagtaattct gctggcggtc gtttacaaaa
     8521 agatacaacc gatcgttgaa ataaacagcc ttttgatgtt gaacatttaa agagtgagcc
     8581 gcatcgttta ccttttgcca aatatacaat tccggttcca cttgatgaac atttaccttg
     8641 atcagatatt tgcgccttgt tactccatca gaagcaatat taataattgc cttgatcttt
     8701 gtaaagtcga aagcttcatt tccggaaata agggctgttg aatcattttc ataaaccaaa
     8761 taggcagcat acgtgctttt aaaggtgaaa gtggcataaa cactgtcaat tcttgtctgg
     8821 tagggtagcg aatcaaggtt gacaatcacc gaatcgtatt ggagactaaa aacagcatct
     8881 tttagattgg gtatcttatt gttggccgaa aaagtaagcg aaacaaaact tggatcagaa
     8941 gaaatttcct ttacattacc atcccccaag caggaattta gaaaaaaaat agtacctaac
     9001 aaagcaagta ttttcaattg atttcgcagc atgaacaata aattttgaat gaaggagcaa
     9061 agttaaaaaa catttcagtt gttgcgccga aaaggtcgat attttattgt tttttattac
     9121 tttagcggat aagtattact taaaattaat taaacatcga gttccaccat gacaggacaa
     9181 tgatcggaat gaaaagcttc gctaaggata gcagccgctt tcagtcgaga cctcagcggt
     9241 tcgcttaccc aatgataatc gatgcgccag ccggcatttc tgtaacgcgc accaccacga
     9301 taactccacc agctatattt ttccggactc tggtcgaaaa cccgaaacga atctaccatt
     9361 cccaaagcct gaaatcgatc catccattcc cgctcttcgg gtagaaaccc tgatactcct
     9421 atttgacgtt cgggatgatt aatgtcaatg ggcttgtggc agatgttgta atcgccacaa
     9481 accaccaaat tgggtctttc acgaagcaag ttttgtataa atttcagaaa ttcttctaga
     9541 aatttcattt tgaaatcctg tctttctccg cctgatgatc cggaaggaac atatacattg
     9601 ataaccgtta tatcgccaaa gtctgtcctt aatactcttc cttcagtgtc aaattcggga
     9661 ttatccattc cgaaataaac ataatcgggc ggaattttag taaaagtagc aacgccactg
     9721 tacccttttt tttgtgccga atgaagataa gcggtataac ccagttcggc aaaaagtagg
     9781 gtgtcgattt ggtcgggctg tgcttttgtt tcctgcagac aaagcacatc aggattctcc
     9841 gcttgcagcc attgaataaa atctttggtc agtgcagctc ttaaaccgtt tacattgtag
     9901 gagataattt tcatggatat tgcttgcttg aggttcaaat tttcagcaaa tgaattaata
     9961 aggattttgt tttaacattt ttaactgttt aaataacttg gatgcgaaaa caaagttatt
    10021 gaagctttca ttctcagcat caaaaatttc atcttttgtg ccttcccaag ctttcactcc
    10081 tttttcgata taaatgattt tttcgccaat ttccattacc gaattcatgt cgtgtgagtt
    10141 gataatggtg gtaatattga attcgtgtgt aatttcataa atcaaatcgt caataatcat
    10201 agaggtaagg ggatcgagtc cggaattggg ttcatcacaa aaaagatatt taggtcgtaa
    10261 agcaatggca cgcgcaatac ccacccgttt ttgcatcccg ccactgattt cggaaggata
    10321 aagatcgtaa gatctttctt cgagattaac gcgacgaagg caaaaataag ctctttccct
    10381 tatttcctct ttactttcat gggcaaacat ttccagtgga tacattacat tttccagcac
    10441 cgtcatggaa tcgaataggg cagctccctg aaaaagcatg cccacttcac gtcgcagcat
    10501 aagtatttct tgaggcttca tttttggtaa atttcgtcca tcgtactcta tgcagccgct
    10561 atcaatttgg tgaaggccga taatacattt catcaaaacc gttttgcccg aaccactttg
    10621 tccgatgatc atatttacct tgccggtttc aaaaacggca gaaatgtcct gcaatgccgg
    10681 aacgccatca aaagttttta ttatgttttt tacttcaatc atgcttggtt tgaagttggt
    10741 ctgttatttt taaaagttat ttctccctta tacaaacatt gtttttagaa aaaaatcttt
    10801 tcattttaaa gtttctgctg aagattgaaa gcgcagaaag tattttttat cccaacatta
    10861 cattggtaat caatagatta aaaaacaaaa ttaagatact gctgttaact acggcgttgg
    10921 tacttgcttt acccacttcc aaagctcctc cgtaagcata atagccataa taggaagaaa
    10981 cggaagaaat gatgaaagca aaaaccagcg atttaaccat tgcataaaac acataaaaag
    11041 ggttaaaggc atattggata cccagcagat aatcggccac cgttatgaca tcgctgaaaa
    11101 cgccgaccaa ataaccgcca attagtccca cagccatgct gaaaattacc aaaaacggca
    11161 tcataacgac gaaagccaca atttttggaa gaataagata attggcagaa ttgacgccca
    11221 taatttcgag tgcatcaatt tgttcggtaa tacgcatggt accaatttcg gaagcgatat
    11281 tggaaccgac tttgccggcc agaatcagac acatgatggt agaagaaaat tcgagcagca
    11341 atgtatcgcg ggttaccaat ccagtgctat aggttggaag taagggattt tccgtattga
    11401 gtttggtttg aatggtcatg atagccccga tgaatacaga aatgattaat actatgggca
    11461 aagattgtaa acccagttta tccaattctt tgggaaattg gcggaaaaac attttcgaac
    11521 gatcaggctg tacaaacaca cgtttcatca gcaaaaaata ttttcccgtt tcttgaaata
    11581 aattcatacg caaattttca aaatttcaag cagttaaaaa attgtcgaca aaccatccaa
    11641 caagttttga agcaaaaagt tcttgttttg tttcgcaaac aagttgcaaa tatacttaat
    11701 ttttgcaatt gatgaattag tacactacta ttcaaaagca aaaaagaatg tgttagaatt
    11761 tcttatttta gcgataaaat caaaataaac agaataataa aatttcacag gcttcagtaa
    11821 aaaataaaca gaatcttgga atctttacga taaagcatgg atttatttac ttgatagcct
    11881 gtctcgtgtc attcggttat tcatgcaata ttaaccgaag gtgcaaaagt agatatattc
    11941 aaaaattatt ttgataagaa taagtttgaa atgggtcagg attatttatg tatcaataaa
    12001 atagaagcaa atcgtgtaag aagggctcgt ttccaaaaaa tttctgattg gggataaagt
    12061 atggaataaa attaaaaagt tcgatcatta gggactaatt ttactttgtc tttttgtttg
    12121 aagatttgca aaagcaactt ttaaaacaca ataagatttt ttaatagttt tttctgtaaa
    12181 agttatggtg tgatgaacaa atttacaagg aaaaatttcg attttacctt gtctgtgtgt
    12241 aaattgaaaa ttttcgtgta actttgcatc ccataaccaa agtaacaaag gcatggaaaa
    12301 aaaggtaatc aaaaaagcat taatatcggt ctatcacaaa gacaatctgg aagaaataat
    12361 caccaaactg catgctgctg gagtagaatt tatttctacc ggaggcacaa aatcgtttat
    12421 tgaatcgctg gacattcctt gcgaagcagt agaagatttg actggctacc cttcaattct
    12481 tggaggaaga gtaaaaacgc tgcatccgaa aatctttgga ggtattctcg cgcggcggga
    12541 caatgctcag gatgtgcagc aaatgtctga atatgaaatt tcgggaatcg atttggtcat
    12601 tgtagatttg tatcccttcg aagccactgt tgcggcaggt gcagatgaac caacaaccat
    12661 tgaaaaaatt gatattggtg gaatttcgct tatccgtgct gcagcaaaaa atttcaagga
    12721 tgtggttgtc atcgcctcca aagagcaata cgaacctttt ctggaaattt taaacagcag
    12781 tggagctcaa acttccatcg aggaacgtcg atggtttgcc aaagaagctt ttgctgtttc
    12841 ctctcagtac gatacagcca ttttcaacta tttcgatgaa ccttatcaaa gtgcttttcg
    12901 ctggtatgaa aatcatccaa aagttttacg ttatggcgaa aatccgcatc aacgcggaat
    12961 tttctttggc aaattcagcg aaatgtttga acaacttcag ggaaaggaaa tttcctacaa
    13021 taatctgctc gacattgacg ccgctgtaaa tcttatcaat gaatttgacg acataacctt
    13081 tgctgttctc aagcacaaca atgcctgcgg aattgcatca cgtccttcac ttcttgaagc
    13141 atggaaagct gcgctggctg gcgatccggt ttctgctttc ggcggaattc tcatcaccaa
    13201 tgccatcatt gacaaagaaa cagctctgga aatcaacaaa atattttttg aagtaatcat
    13261 tgctcccgat tatgatttgg aagccattga aatattaagt caaaagaaaa atcggattat
    13321 tttgataatg aaaaacaata cactgcgtcc caagcaattc cgttccttac tgaatggagt
    13381 gctgatgcag gatagagact tgcataccga aacggctgct gacttaaagg tagttaccga
    13441 aaaatcgcct tcagaagctg aaattgaaga tttacttttt gcaaataaaa ttgtaaaaca
    13501 ttcaaaatca aatgccattg tactggcgaa aaacaaacaa ttgtgtgcca gtggtgtcgg
    13561 acaaacttcg agagtggatg cattggttca tgccattgac aaggcgcgtt ctttcgattt
    13621 cgacttaaaa ggtgccgtca tggcttccga tgcctttttc ccgtttcccg actgtgttca
    13681 aatagcacat gaagcaggaa tcactgccgt cattcaaccg ggtggttcca tcaaagacca
    13741 ggactccatc gatttctgca atcaaaaggg tctttcgatg gtatttacag gttatcggca
    13801 ttttaaacat tgacaatttg caaataaaaa ttttcttaaa aacataaaca cacaaacaaa
    13861 tgggtttttt ttcatttaca caggaaatag ccatcgattt aggtactgcc aacagcatta
    13921 tcgtttacaa tgatcagata gtggtggacg aaccgtctgt tgtggctttg gacaaacgta
    13981 ctgaccgatt aatagccgta ggagaacgtg cacgccagat gcagggaaag gaaaacgagt
    14041 cgattcgtac catacggcct ttacgcgatg gggtaatagc cgatttttat gctgccgaaa
    14101 tgatgattcg gggacttatc aaaatgattc cttcaaaaaa tcgttttttc actccttcct
    14161 taaaaatggt cattggtatc ccatcaggca gcactgaagt agaaatgaga gccgtcaggg
    14221 attcttcaga acatgcggga gggcgcgaaa tatatatgat ttacgaacca atggcagccg
    14281 ctttgggaat tggaatcgac gtggaagctc ccgacggctg catggtggta gatattggtg
    14341 gaggaacaac agaaatagcc gtaatttcat tgggcggaat tgttttgaac aagtctattc
    14401 gcattgccgg agacgatctg accgacgata ttgtggaata tatgggacga atgcacaata
    14461 ttaaaattgg agaacgtacc gccgaattga taaaaattaa tgttggagct gcattgaccg
    14521 atttggaaga tgctccggaa gactatattg ttcgcggacc aaacagaatg acggcattgc
    14581 ccattgaagt tcctgtatct tatcaggaaa ttgcacattg tctcgaaaaa tcaatttcaa
    14641 aaattgaagc agcaatatta agcgcattgg aactgacgcc tcccgaactc tattccgaca
    14701 ttgttcagca tggtattttt ctttcgggtg gcggagcttt gctgcgtgga ttagacaaaa
    14761 gactcacgga taaaatcaac attccttttc atattgccga tgacccgctt catgcagtag
    14821 cacgcggcac cggaatagct ttgaaaaatg tcgataaatt ctctttcttg ttaagataaa
    14881 attgttaata ttcgattctg ccggactaca agaatgtatt ttcttactgc tccaaagaat
    14941 cggatatttt tgtgtttgca gaaaaaagcc gcaaagatta caaacttttt atgaaaaatc
    15001 tcattaattt tttgattcat catagcggat tttttgtatt cttgctgttg gaaatccttt
    15061 cactgatgct ggttttcaat aacagagatt atcagaaaag tgtatttttt tcttccagca
    15121 acaccataat ttcttctttt tacaactgga acaattatat tgtcggcttt ttcaatttaa
    15181 gcaaatcgaa tcagcaactg gccgaagaaa acacacaact caaaaatcag ttgctcgact
    15241 tgcaaaataa actggaagcg ttgcaaccgt cagggactga ttcgctttcg tacaaaatcc
    15301 ctccggaaaa gcaataccga tttattgaag caaaagtgat caacaattct accaataaac
    15361 tgcaaaatta tattacgttg aacaagggag ctttggatgg cgtgaaaccg gatatgggag
    15421 taatcaacga cgaaggcata gtaggcattg taaaaaatgt ttctcagcat ttttccgttg
    15481 taattccggt tttaaatccc aaaattcaaa tcaattgcaa atttaagcga aacaattaca
    15541 ttggttcgct tgtatgggat ggaaaagatt atcgctttgc ctcgttgacc gatatagcgc
    15601 gacatgttcc gataaaaatg ggagacacat tggttaccag cgggctgacg actactttcc
    15661 ccgaaggtat tccggtaggc accatagaaa attttaacct caaggaaagc gatgcctatt
    15721 atgaaatcaa agtgaagctg gcaacaaatt ttcgtaccct ttcctatgtt aaggtgattg
    15781 atttcaaaaa tgccggcgaa ttaaaagagc ttgaaaaaac agcatacgaa gaataagatg
    15841 gcaattacgg tttttcaaaa tattattcgt ttcgttttac tgatgatggt tcagattttc
    15901 gtactgaaca acattcagtt aagtgggtac atcaatccct atatttacat tcttttcatt
    15961 ctttcgttgc cctatcgcac acctcagtgg ttaactttga ttcttgcttt tgtaaccgga
    16021 ctcatggtgg atatttttat gaacacaatg ggaacacatg cttttgcaac tgttttaatg
    16081 gctttcacca gaaacggaat catcaatttg attaccgatt tcgaaaaaga tgaaaatcct
    16141 actccatctt tctatacttt tggcattggc gtgtatatca gatatattgt attgcttgtt
    16201 ttcatacatc ataccgccct tttttttctg gaagcatttt cactagctca ctttggcatt
    16261 gttatcaaaa aaattttttt tagctcagtt gtatcaattt tgctaatttt gggcattcaa
    16321 tcgctgaaga aaaaataatc aatgataaac gattctttac agaacagaag atacgtaatt
    16381 gcaggcatca tttctgtagt cattgccctg tacatcattc gtttgttctc tattcaggta
    16441 atagaaacaa aatataaaca gggagcagtc agcaatgcat ttctgaataa aacactttat
    16501 ccgcccagag gtttaattta tgacagaaat caaaacttgc tggtttacaa caaacctgcc
    16561 tatgacatag ccgtcatcat gcgcgaaatt cacgacttgg acacgctggc attttgtcgt
    16621 gctctgaata tagaccaaac gtttttcaat aaacgaatgg ccgaaatcaa ggacagaaaa
    16681 aagaatcttg gctattctgc ctataccccg caattgtttt tgactcaact cgatattcag
    16741 gatatagcta ccattcagca aatgatgtac aaatttccgg gattttacat ccaaagccga
    16801 actttgcgcg aatatgccta tccttatgcc gcacacgttt tgggaagtat tggagaaatt
    16861 tcacaatcgc gattggatga agacaactat tatgttagag gcgattatgt tggaagagat
    16921 gggttggaat acacctacga aaaagatctc agaggagaaa aaggagttga agtcttgctg
    16981 cgtgatgcaa aaggcagaat aaagggaaaa tacgaaaacg ggaaacacga tgtggcatcc
    17041 aaaacaggga aaaatcttat tacgacgctt gacatacagc ttcaaatgct gggagaacag
    17101 ttgttgaacg ggaaaatagg tggaattgtt gccatcgaac ccaaaaccgg cgaaatattg
    17161 gctatggttt ccagtcctac ttttaatcct tcttcacttg tgggaaggca acgaacaaaa
    17221 aattacataa aactggcaaa tgaccctgcc aaacctttgt tgaatagggc tacacaagcg
    17281 caatactcac ccggttctac ctttaaaact attcaggcac tggtatgtca gcaaatggga
    17341 ggaatcgata cgaaaacgct ttttccttgt aatggacctg catcctctcc catcaaatgc
    17401 acgcatcatc atggttcacc ggttaatttg cttaatgcta tagagcaaag ctgcaatcct
    17461 tatttttggg gagcttttcg taccacattg gaaaaagacg gatacggcga aaaaaacaaa
    17521 aattttaagc gaaattatca aatctggcga agttatgtat taagtttcgg ctttggaaaa
    17581 aaattcaccg atggcgacat ctatcagcaa tcttcaggca atattcccac cgaaaaatat
    17641 ttcaatcgct attttgggga aaccggatgg agagcaatga ccattcgatc attagccatt
    17701 ggtcagggtg aaattcttgt tactcctctt cagttgtcaa atgccatggc tattattgcc
    17761 aacaagggtt atttcgttac tcctcatctc aacaaagccg actcgttgtt aaatagagtt
    17821 catcgtacca gcgtggaaaa aaattatttc gacatagtag atgaaggaat gtggcgggtt
    17881 tgcgaatttg gaacagcgcg acactacaaa gttcccaatt tgtccatgtg cggaaaaaca
    17941 ggaaccgtac aaaacagtcg gggaaaagat cattctattt ttgttggata tgcgccaaga
    18001 gaaaatcccc aaattgcaat agccgttgtg gtggaaaatg cgggatttgg agcaacatgg
    18061 gcgctcccaa ttgccagttt aatgattgag cgatatatta acggaaaaat cgaacgtacc
    18121 gatttgctgg aacgaattgc atcggcaact ctttcagctt ccgcaactag attaacaaat
    18181 aaaaccaatc ttacagattt tatcaatggc caggacttct aatatcaaat acgactggat
    18241 aaccatcatt tactacgttt tgctggtttt tatgggttgg cttagcattt atggtgcaag
    18301 ttacgacttc gaacatccgg caatttttga cttcagtcag cgtgcaggga aacaatttat
    18361 ttggatactg acggctttcg gcattgcagg cattctattg ctaattgatg accggattta
    18421 caactatttc agctacttta tttacggatt cactatttta ttgcttattg ccacaatttt
    18481 tgttgctcag gacatcaaag gttcgcgttc gtggattgtc ataggtccca ttagctttca
    18541 gcctgccgaa attgcaaaaa tgtccacatc tcttgcgttg gccaagttta tgaacacata
    18601 tggtttcaaa ttgcgatcgt ggaaaaactt gattcctttg ttggtaatcg tatttattcc
    18661 tttgattctc attatgctgc aaaatgaaac cggatcggct ctggtttttt tgtcgttttt
    18721 tctgatgttc tatcgagagg gaatgaatgg aattgtttta ttgattggtg ctttagctgc
    18781 atttttcttt gttgtggtta ttcgtttcag catcgttcca attgccttcg gcacaggcac
    18841 attgggaatt gttttggcta tgacgttaat tattgttgga cagtttatat atgcttattt
    18901 ttttgttaaa aataaaaaag aagcgcttta tttgttgggt ggaagttcat tggttgcttt
    18961 ggttgcctat ctgcttagtc tgaaatttaa agtgaatttc aattcggtag cttttttcac
    19021 agtgctggct tcctgcgtat actggatggt gatggagctg aaatttcgcc ataaaaagtt
    19081 cctttatctt tcgctgattt cacttggttt ttcacttttc agtttttcgt cagattatat
    19141 ttttgaaaag acactcgaac cgcatcaaca aacccgtatt aaagttttgc tgaatatgga
    19201 aaacgatcct atgggagcgg gttataatat caatcagtcg aaaattgcca ttggttcagg
    19261 tggtttttgg ggaaaaggtt ttttgaacgg gacccaaacc aaattgaaat acgttcccga
    19321 acaggatacc gatttcattt tttgtaccgt tggagaagag catggttttt tcggttccac
    19381 gatagtactt attttatatt ggctttttct gatgagactt ataagtattg ccgaacggca
    19441 gcgagaaacc ttcaacaggg tatatgcata ttgtgtagtg agtattttct ttttccatat
    19501 tatggttaac attggtatgg tactcggcat tatgcctgtt attgggattc ctttgccatt
    19561 tttcagttat ggcggctctt ccttgtgggg ttttacgatt ctacttttta ttcttttgcg
    19621 actcgacgct tcaagatttg aacatattca ttgatacaaa tcaataatat tttttatatt
    19681 tacgccgata ttttggataa tttattgaat caacacttat attcgttttt attaaaatcc
    19741 attgaaaaca atattatgaa aaagttaatt atcatttcat tgccgcttat tttattttta
    19801 ttttctatga aagcgtcatc ccaaaaattt gaaaaagacg tattttccac atcagcggga
    19861 aatttaacaa tcacgtttat cggacatgga acattgatgt ttgaaatcga caataaagtt
    19921 atacacattg accccactgc catgtttgcc gattattcta ctcttcccaa agccgatttg
    19981 atacttatta cacatgaaca tgccgatcat ttcgatatgg ctaccatcga caaaatcagt
    20041 aaaaaggaaa ccgaaatcat tcttaccaca acctgcaaaa atcaactgca aaaaggaaaa
    20101 gttatgaaaa acggggactc ttacaattgg aagaagttta ttcatattca agctgttccg
    20161 gcttacaaca ttcagcacaa gacaaatact ggccaactct tccatcccaa agggcaagga
    20221 aacggttata tattaacgtt tggaaatttt aaagtttatg tggccggcga caccgaaaac
    20281 atacctgaaa tgaaatcttt gaaaaacatt gatgtagctt ttctacccat gaacctgccc
    20341 tacacaatga cgcccaaaat gaccgccgac gcagccctta gctttatgcc aaaaatttta
    20401 tatccctatc attacggtaa taccgataca aacgaattgg taaatctgct gaaaaacaga
    20461 cccgaaattg aagtgagaat tcgaaagttg aattaagatt gatttataaa aatgtttctc
    20521 ttctaaacaa aaatattgtt tcacaaatta aaaaaacaat tccatgaaaa acagaatcgt
    20581 tgaagcagcc attattgctg ttggaataat ttgtctggga ctctgcatcc gaaacggcat
    20641 taaaattttc tcacaaaaag accgcgcagt ggaagtaaaa ggacttgcag aaatggaagt
    20701 tcctgccaac aaggtgattt gccccattct ttacaaggat ttgggaaatg accttgctca
    20761 gctttatgta gatatgaata aaaagaatca ggccattatt tctttcataa aggaaaacgg
    20821 aattcaggaa aatgaaatat cactttcggc accggaaatt gtggacatgg cagccgaaag
    20881 atttgtagaa aataaatcgc cttatcgata caatttgact tcggtcatta caattagcac
    20941 accaaatgtg gataaggtac ttgctttaat gtcgcgtcaa gctgaacttc tcaagaaagg
    21001 aatagctgtt acggtcaaca attacagcgt tcgatacgaa tatacgaaac tgaatgaaat
    21061 taagccccaa atgatagagg aggcaacaaa aaacgctcga aaagcagccg aaaaatttgc
    21121 caaggattca gggagcaaat taggaaaaat taaaagcgcc actcagggtc agtttattat
    21181 ttccgacaga gacgagaaca cgccttacat aaaaaatgtc cgcgttgttt ctaccataac
    21241 ctattatttg gaagactaaa aaatttttta taattatccg tattgcgata tattataatg
    21301 attatccgca atgattttta ttgtaaattc ttgacttgaa atcggagtgt agtttacggc
    21361 agcaataagc aatctgtaga agataacttc ttgaaaataa cgttaattga atttatctca
    21421 gaaccaattt gtttactaaa actgctaaaa tagagtaaac tgaaaagcga aaaaatcgtt
    21481 gttctcgaaa acttgtgtta ttgacgacat gcacaaaaaa aggtaacgtt tagcatatta
    21541 cctttttttt aaaaaaatca aagtgaagaa atttactttt caatcagttt ttcaacttct
    21601 ttccaattta ccacattcca aaaggctttg acataggatg ctcgttttga ctgatatttc
    21661 agataatacg catgttccca tacatcgagc gtcaaaaccg gctttccttt taccgaagaa
    21721 aatggcatta aggtattatc ctgattggaa gtggaaatga ttctcaattt tcctgaagat
    21781 tcttttatca gccatgccca tcctgaagca aattgtccta atgcagcttt ttcaaattcg
    21841 ttcttaaaat tatcgatgct gccgaaattt ttaatcagta ttttttcgag tttgggagaa
    21901 atggttgtgg aaccggccgg tgccagcatc gaccagaaaa gacaatgatt gtaataacct
    21961 ccaccattgt tgcgtaccga caattgaata tcagcaggca attcgtttat attttccaac
    22021 aattctacga tatccttctt gtaaagttca ggatattttt cgagtgtttt attcaaattg
    22081 tttgcgtaag tcgcatgatg gttgttgtag tgaatgtaca tggttgtact gtcaatatac
    22141 ggttcaagat cgctgtagcc ataatcaagt tttggaagct gaaattgtcc gaaagccatg
    22201 accatagctc caaataatgc tatgaatgat aatgttaagg atttcatttt tataatgttt
    22261 ttagagttat tatatttata aacaacagac ttatttcctt tgttcagtga tgaaaaaaaa
    22321 tctataaggt tgatgccatt ttttaccatc atggaagtcg gcttttcttc cttttaacgt
    22381 tgtaattgta atttttaatc aaatacagcg ttattttaat cagtacttct gtcaatgggt
    22441 tggaggttgt cttttggagc gtatgtaaat aaatttgtat ttttacacac aaaaactctt
    22501 ttttaagttt tgtcggatga ttgattatat taaggggcaa atagttgagt tgaccccaac
    22561 ttatgtggtg ctggaggcaa acggactggg atatttcatt tcaatccctt tgccggccta
    22621 tactttattg agtcatcaat tggaagcaaa gctttttgtg cacgaagtag tgcgggaaga
    22681 tgcttatttg ttttttggtt tcattaccaa aaaggagaga gaagtttttc aacttttaat
    22741 ttccgtttcg ggcataggcg taaatactgc cagaatgatt atgtcttctt attcggtaga
    22801 agaaattcag gaaataattg ccgccggcaa tgtgtcggcg ctgaatgcca ttaaaggcat
    22861 tggcagcaaa acggcccagc gtattattgt ggatttgaag gacaaaattt taaaagggaa
    22921 agatgatggt ggttcgctgt ctttgctttc cgacaaaaat gcccaaataa aggaagaggc
    22981 agtgtcagca ttggtcatgc ttggttttcc ggtcggtgct tcgcagaaag caatagaaaa
    23041 tatcttgaaa gaacagtctc agcttgaagt tgaacaattg atcaaaatgg ccctgaaaat
    23101 tttgtgaggg tcagcaaaat ttgaaattca agaaaacttc atttcagtga agacaaattg
    23161 ttaagaacaa ggaatagaaa ttttatatga caaatagaaa aaaatcatct tcgatttttg
    23221 tttatttcgg tatttttttg agttcgacag ttggatttgt ttatgcttcc ctgcaacaac
    23281 ccgttccggc cgaaacccgt tccaatgctc tgaaacagca agattccgtt cagcaacatt
    23341 ttcctgtcaa aaaattggtg caggatacct acgaagatat cactgctact taccctctcg
    23401 acgcacccgt tccttcaaat attaaaacgg aaattgagta cgacccctca acagccagtt
    23461 atattttgcg tacttatttg ggaaacatcg aaatagctac tccgtttcga atgacggaac
    23521 aggaatatct cgattattcc ggaaggcaag aaatgcaacg ttattggtac gaaaaaaatt
    23581 cttctgcaca acaaaacgtt gaagacaaat tcagtgttac ggacatgaaa ttcgatattg
    23641 gccctgccga taaaatattt ggtcccggtg gagttcaaat cagaacacaa ggttctgcag
    23701 aaatttctat gggtttcaaa accaacaaaa tcgacaatcc ggcattgact gagcggatgc
    23761 gcaaaagtac tattctcgac tttgacgaga aaattcagat gaatgtaaat ggcaaggttg
    23821 gcgacaaggt gaacttcaat atgaattaca ataccgagtc gagtttcgat tttgaccaga
    23881 aattgttaaa actgaattat aaaggcaagg aagacgatat cattaaaaac attgaaattg
    23941 gtaatgtcag catgccgtta aacagttctc tcattaccgg aagcacggca ctgttcggtg
    24001 ttaaaaccga tttgcagttt ggcaagttaa gcattagcgc cgtagcttct cagcaacaat
    24061 cgcagacgca aaccgtaagt tcaaaaagag gagcacaaac taccgattac gaaattggta
    24121 ttgatgatta tgatgaaaac cggcatttct ttttgggaca ctatttcaga aacaattttg
    24181 aaaatgccat gagcaagctc ccatttgtaa cttcaggtgt taccattaca cgtgttgaag
    24241 tttgggtaac caacaaacgt ggaaattatg atcaggcaag aaacattgtt gcatttatgg
    24301 atttggggga aacacagcga cttgacaatc attattggaa tgtgctttca tccacatctt
    24361 taccacaaaa caaagccaat tctctttatg atgaagttac ttctattcct aatgttcgcg
    24421 acatacggca gaccaacaat gtactttctt ctcaatatgc cgccgatagt atttttggag
    24481 gagaagatta cgaaaaaatt gaaagtgccc gacggctcga tccttcggaa tataccttga
    24541 attcttcttt gggttatatt tcgttacgta ccgctttgaa tcccgatgaa gtgttggctg
    24601 ttgcctatga atatacttac ggaggacagg tttatcaggt aggagaattt tctaccgatg
    24661 ctgtaactgc tccgaatgct ttgattgtta aacttttgaa aagtactgca cagtctcctt
    24721 cacttgccac ttgggatttg atgatgaaaa acgtctataa tttgggcgct ctgcagattc
    24781 aaccagaaaa ttttcagttg gatattgttt atcgaaacga ttcggtggga accgatctga
    24841 aatatattac cgaaggaaac atacaaaatc agcttttgct gcgtgtgatg aatctcgatc
    24901 ggcttaacac aaaaaatgca gctcagcccg acggaaaatt tgattatgtg gaaggctaca
    24961 ctgtcctttc ttccagcgga agaattattt tccctgtgct cgaacctttt ggttcccatt
    25021 tgcggaaagc gattggcaat gatcagattg ccgaaaaata tgtttttcag gaactttatg
    25081 attctacatt ggtagcggct cgtgaataca gcgaaaaaaa caaatttcgt attgtgggag
    25141 aatacaaggc cagttcggga aacgaaattc ggctgaatgc catgaatata ccgcgaggct
    25201 ccgttaccgt aacggcaggt ggagttactc ttgttgaaaa tgttgactat actgttgatt
    25261 acacgatggg aacggtcaac attatcaata cttccatttt ggaatcaggt agcaatattg
    25321 atgtcaaatt ggaaaatcag tcgatgttca gcatgcaacg aaaatcgttg gtaggcacac
    25381 atttggaata tcagttcaat cgtgattttt cgttgggtgc taccgtgatg catctttccg
    25441 aaatgccttt aaccactaaa gtcaacacag gcgatgaacc catttccaat accatttggg
    25501 ggctcaatac ttcgtggaga aaggaatctc aatggctcac caaccagctc gacaagctgc
    25561 cttttgttaa tgccaccaag ccttcaacat tggctctgaa cgccgaattt gcccagttga
    25621 ttcccggaca ttcaaatgtt atcagttcgg cggggctggc ctatatcgac gattttgaat
    25681 cgaccaaaac caccatagac cttcattatc cggtattctg gcatcttggc agcacgcctt
    25741 ttgaccccaa tggtggactt tttcctgaag ccgcactgag caacaatgtg gattatggta
    25801 aaaatagagc cttactggca tggtactatg tggatccgat attgaatgcc aacagtcgta
    25861 atactcccga aaatttaaga aacaatctgg aatcacaatc caatcattta acgcgcaatg
    25921 tgttggaaga agaaattttt ccgaataaag aaaggcttgc aacgcaaaca tcccgaatga
    25981 ccattatgaa tctttccttt tatcctaacg aaagaggtcc gtacaatcta gactgggaag
    26041 gcataaatga agatggttcg ttgaaaaatc ccgaaaaaag atggggtggt atcatgcgta
    26101 aactggacgt aacggatttt gaaaattcca acatagaata tatcgaattt tggatgatgg
    26161 atcctttcat taccgataaa gatggcacgc agaaaggtgg tgatttgtat ataaatttgg
    26221 gggatatcag tgaggatatt ttaaaagatg ggaaaaagtt ttttgaacag ggcttgagta
    26281 tcgatggcga tgttaccaaa agcgatacta ccgtgtgggg aagagttccg aaaacgcaat
    26341 caacggtaaa ggcttttgac aatacaccgg gagcgagaaa atatcaggat gttggtttaa
    26401 atggactatc caccgatgaa gaatttgaat atcctactta caaaaaatat gtagaacaaa
    26461 ttacacgaaa aatcagtcct caaaccctcc aaaaatggca agacgaccaa ttttcgccat
    26521 tgaatgatcc tgcaggcgac aactatcatt tttatcgtgg ttccgattat gacgaacaga
    26581 agaaagatat tctttcccga tacaagcatt ataatgggac ggaaggaaat tcacctgaag
    26641 cagaaaatgt taacgaaagt tatacaactt cagctacttc gcttcccgac atggaagatt
    26701 tgaatgaaga caataccatg aacgaatacg aaaaatatta tcagtatcgg gttcagattc
    26761 gcagagatgc ttttcaggta gggaaaaatt atatagccga caaaattact tccaatgtaa
    26821 agttggccaa tggaaacgtt gaacctgtaa cttggtatca gttccgtatt cccatccgtg
    26881 agtacaacga taaaatcggg agcattcgca attttaaatc cattcgtttt atcagaatgt
    26941 ttcttaccga ttttgaaaaa gacattcatt tgcgttttgc tactttcgat ttggtaaggg
    27001 gtgaatggcg aagttacacc aagtcattgt atccgaatgg actgactccc ccttcggaag
    27061 ggaaattgga agttcaggct gtgaatattg aagaaaacgc cgatcgtacc ccagtaaatt
    27121 atgtattgcc tcctggagtt accagagaaa ctgatccggg acaaccacag ctattacagt
    27181 tgaacgaaca atcgatggtt ttaagagtca gagatttggc gccaaatgat gccaaagctg
    27241 tttataaaac tacttcatat gatatgcgtc agtacaagcg tcttcaaatg tttgtacatg
    27301 cagaaaaatt gattgacgat atttccaatt tgcaggataa cgaattgacc tgtttcatcc
    27361 gaatcggaac cgatatggtg aacaattact atgaatacga aattcccttg cgattgacgc
    27421 ctcacggaaa atatctgggt gaaaatacag agcagcggga gattgtatgg tatcctgaaa
    27481 acatgtttga ttttgcattt tcaaaactga ttaatgtcaa actccaacgc aacaagactc
    27541 ttcaggccag cgcaggaaat gtttcgaatc ttgtgcctta taccatattt gatcccgata
    27601 agccaaaaaa tagaattacg gttgtgggaa atcctaccat ttccgatgtt cagaatatta
    27661 tgattggcgt tcgcaacaga agtggcagca taaaatcggg agaaatttgg gtaaacgaat
    27721 tacgcatgtc ggaatttgat gaatcgggtg gttgggctgc aatgggaaat ttggcagtcg
    27781 gattatcgga cattgggaca ctgaatcttt cgggaagaat tgaaacttcg ggttatggga
    27841 gtcttgaaag taatgttatg aatagaaatc tggaagattt gcgtcagatg aattttgcag
    27901 ctgccataga attgggcaga tttttacctg aacgggctaa attgcagatt cctacttatt
    27961 tctcgtattc caacgaaacc gtatcgccaa aatataatcc gctcgatcag gatgtcgagc
    28021 tgtccgatgc tttggataat cttcccacaa aattagatcg ggattcctta atgaatttgt
    28081 cgcaaacggt gagcacttct aaaagtttta atgttgccaa tgccaaatta aatataaaaa
    28141 gtaaaaaacc acagttttat gaccctgcaa atttgaagtt tacctatgcc tatattgaaa
    28201 acaatgaaca taattcggag attgagcaaa atttgataaa aaaccagcgt gctgcagtcg
    28261 attataattt caactttgca gcccagccgt gggaaccgtt taaagatgta aaagctttga
    28321 acagtccggc atttaaaatc atcaaggatt ttaatctgta ttatctgcct tcttctttaa
    28381 attataacgc aaatcttaat cgatcgtatt ctcaaatcaa acttcggagt tttgatgttt
    28441 attcgggcag ctataccaac gatgaattgt ccaatctcac tttcagcaaa gattttatgt
    28501 tcaatcggca gttcgatatc aaatatgatt tgactcgttc gttacatttc agttttcaaa
    28561 cagtaatgaa cgccaacatc gatgaaccct attttacgcc cgaaatgggg aaggagtatt
    28621 acgaagcatg gcgcgataca gtgtggcaca atatcaaaaa acttggaaca ccctatgttt
    28681 atcagcaggt atttactgct tcttggaatg ttccactcaa taaaataccc attacgaact
    28741 ggatgcaagc aaatgtttcc tataattcca attacaactg gaatcgaagt gcacaaatgg
    28801 aaggaggatc caatattgga aatacagcta ccagcaccgg ttcgtggcag tttgatggac
    28861 aactgaattt tgaaaccctt tacaataaat ccaagtttct gaaaaacgtt ttgaaccgaa
    28921 ataaagttac tagtgccaga cctcagcctt tcaaaccggg aaattatact cagaccgttc
    28981 aactcaagtc cgaccagccg ctcaccattc agcatcgctt gggcagtgag cgcatcaggc
    29041 tgacagcaaa agataaggat ggaaattctg tgttactctc ctataaagta aaaaatgctt
    29101 cttcggtaga gattttaccc aaacaaaata tggacagcat cacaatcaac attgtcagtc
    29161 tggatcccaa tcagcaatcg gtagtaaaca aaacgcgtga tattacattg ggtgctttaa
    29221 tgatggttcg acggttttct atcaattatc gagaaaacaa cagcacggta ttgtcgggat
    29281 ttatgcctga aactggattt atggggcagc agaattacaa tggtatcgat gctcccggat
    29341 atacgtttgc ttttggattt caggaaaaag attttataaa aaaagctttc gaaagaggtt
    29401 ggctgaatgc aggtgattct gtggttaatc cggccacaac tgcttttact tccgatcttg
    29461 atatcaaagc aacgctggaa cctattccgg gctttaaaat tgatttgaat gggcggcgat
    29521 acacggcttc aaatacttcg gtgttctaca tgtacgacgg tatgccgact acttacagcg
    29581 gaagttttaa tattacgcaa attggcttgt ccactgcctt taaaaagctg ggaaacgcta
    29641 ccgacaatta caattcggaa acttttaatg ctttccttgc taatcggcaa aaagttgccg
    29701 accgcctgaa tcagaaatat ataggcacac gatatccaca aagtggattt atcaaatctt
    29761 ccaatttggc aggcaaagaa tatgatccat cattgggtgc atatcagctg aattctgccg
    29821 atgtgttgat tccagctttt ctggctgctt ataccggacg tgatgctaca aaaatttcta
    29881 ccaatccgtt cctttctatt tcgagtattt tgcccaactg gcgaatcagc tatgatggac
    29941 tttcaaaaat agaatggttt aaaaatcatt tcagaaatgt gaatctgacc catgcctatt
    30001 cttttcgtta cagcattggt tcctattctt cttattctac atgggtgcct atggaggatg
    30061 aaagttcgtt gggttatgtt cgcgatgttc aaaccgacaa tccgattcct tcgagtgctt
    30121 acgattttac tagtgtttcg ctcaacgaac agtttagtcc acttctgggt atcaatgcta
    30181 ccatgaacaa cagtttgtcg gcaaaaatcg aataccgcaa gcaaagaaac cttgctttga
    30241 atctttccag cactcagctg atagaaagtg ccagcgacga atatgttttg ggatttggtt
    30301 atctgttgaa ggattttgat gtgattttgc gattgaaatc ggacaagcag tcgaaaatca
    30361 aaaacgattt aaaactttcg gccgatattt cctataaaga tattaaaacc ttgttgcgca
    30421 aagtggagga gaatattacg caggcttcga gtggaaataa gatgataacc ataaaattcc
    30481 tggccgatta cgtattcagt tcgaaagtta atatccagct ttattacgac catcagtcga
    30541 gtaccccact tgtttcctct tcatttccgg tttcatcttc caattttggg gctagtctca
    30601 ggtttatgct gaccagataa aagtgattac atgttgtttg ttgaattgtt aaattttttg
    30661 aatatcaaac ctcaattaaa ctttggctga ttattattgt tggaaggtta atagaaattt
    30721 taatcacgtg aaatgaagaa aagatttttt ctggtactgt gttgcttttt cgggttcttg
    30781 ttttccgaag cccaaatttt tgaaccgata cattgggata tcagtcataa actgaccgga
    30841 aaaactacgg ccgacattat tttcaaagct tccatagatg aagggtggca tttatacggg
    30901 ttatctttac ctccaaatgg tccgaaacct accgcaatag tttttgaaaa aattgaaaat
    30961 gcccgcaagg ttggtgaatt gcaagccgtt tcgaacttgg agaaaaagtt cgatccaaac
    31021 tttggcatgg agctgaactg gtttacgaaa gaggctgttt tcattcagaa aattacattc
    31081 tcttccaagc aaaatgttcg tttggaaggg tatgtagaat acatggcctg taacgacaaa
    31141 agttgtttgc ctcccacttc cgaaccgttt tcgattcgat tgagtaccac tgaatttaat
    31201 tccatcaact ccggggagca agcaccttct tcggaatccg aaaaaattca gccaacggaa
    31261 gatttcaaaa atgccaaaca agtagattta agttccgaag acactgcctt aacttcttct
    31321 gaatggtgga caccggtagt gaaagagttg aacagctttg ggaatgtggt ttatcataag
    31381 gaaacgactt cgctttggta cattttgctg gccggtttat tgggcggatt tctggctttg
    31441 cttactccat gtgtttggcc aattattccc atgacggtaa gtttcttcct gaaacgttct
    31501 gccgttcctt cacgcggaag aaaagatgca gttttatatg gtttgtccat tatagtcatt
    31561 tatgtggcat tgggattgtt gataacgctt attttcggcg ccagtgcatt gaacagcatg
    31621 tccaccaatg ccatttttaa tttgtttttc tttgttttgc ttgttgtttt tgccttgtcg
    31681 ttttttggtg ctttcgagct gactttgcct tcatcgtggt caacaaaagt ggatgccaag
    31741 tccgaatcga cggtaggttt gctgagtatt ttgttaatgg cttttacttt ggtgttggtt
    31801 tccttttcct gcaccggtcc tattataggg actttgctgg ttgaagttgc atccaccggt
    31861 tctttgcttg ctccggctgt agggatgttt ggatttgctt ttgcgttggc tgttcctttc
    31921 actttgtttg cattcttccc ttcgtggctc gaatcgttgc cacgctcagg cggttggctg
    31981 aacagagtaa aagtggtatt ggctttcatc gaacttgctt ttgcactgaa atttctttcg
    32041 gttgccgatt tggcttatgg ctggcatatt ttggacagag aagtatttgt ttcgctgtgg
    32101 attataattt ttgctttgct gggtatttat ttgctcggga aaattcgttt tccacacgat
    32161 agcgagtcga aacatacttc tgttggtgga cttatgttgg gaatcatttc tttggctttt
    32221 gccgtttaca tggttcccgg actttgggga gcaccgctga aatcgatcag cgctttcgct
    32281 ccgccacttt atacgcagga ttttaattta tacaaagatg aagttcatcc agcttttacc
    32341 gattttgagg aaggaatggc ttatgctgca atgaaaaaac tgccggttat tattgacttt
    32401 tcgggttatg gttgtgtaaa ttgccggaaa atggaagctt cggtttggac tgatccacag
    32461 gtgaaagcat tgctcgacaa aaattttgtg ctgatttcgc tgttcgtcga tgataaaacg
    32521 ccattggaaa aaccttttga aattaacgaa aatggtaaaa caagaaaaat taaaaccgtt
    32581 ggtgataagt ggagctatct gcaacgatat aaatttggag ccaacgcaca acctttttat
    32641 gttatgatag actatcacgg aaaacctctg aataaaccct atgtttttga caccaatcct
    32701 tctcattttg tcaattggtt gaatcgcaaa tagtacattt tgtcttaatc agccctatgt
    32761 gtttttattt ttctgttatt ttgtcattta attcattgtt tggtagaaac attgtattca
    32821 aaatagttga ttggcaaact tattgtttaa tatgagaatg agaaattttt tgagaaactt
    32881 atcagagaat tgtcttgtaa ttctgattta gaaaaaataa gttggaatgg attttttgtg
    32941 ttatatagaa attgattagc tgaacttaag agtttaccaa ttaaaaaagg gaggaaaaaa
    33001 tggaaaagga aaataaagcg tcagaaaaaa atcaatctgc cgaaaacgaa aaaaaacatc
    33061 agcaacaaca taaggaaaat gaagacaaca taattccaca atcggaaaat agtgaagcag
    33121 aaaatactga aaaagagttg gagcgattga gggaagaatg cgaagatttg gcagacaaga
    33181 atttgcgttt gttggccgag tttgacaatt atcgcaaacg tatgctcaag gaaaaagccg
    33241 atttgctgaa aaatggcgga gaaaatgtgt tggtgaatat tttgccgctt gtcgatgact
    33301 ttgaacgggc tttgaaagct attgaaacca gcgatgacat tgaagctgtg aaagaaggaa
    33361 ttcatttgat ttacggtaag tttgtcgatt ttttgaacaa aaataatgtg aaggaaattc
    33421 ctactgaaaa tgtagaattt aacactcaat atcacgaagc agtatccaaa attccggcac
    33481 cttcggaaga attaaaaggg aaagtaattg agtgtttatc gaaaggctac acgttgaacg
    33541 acaaggtgat tcgatatgca aaagtcgtag taggcgaata attttattcg ccttttaatt
    33601 ttttgaattt tttttgttta caaatatgtt gaggatgagg gagaattaag ttttatggca
    33661 aaaagagatt attacgaaat cttaggtgtt tcacgaacag ccacagaagt agaaattaag
    33721 aaagcatacc gtcagaaagc cattcagttt catccggata agaatccggg cgacaagaag
    33781 gctgaagaaa acttcaagga agctgctgaa gcttacgaag ttttaagtga tcctgaaaaa
    33841 cgtgaacgct acgatcgcta tggacatgcg ggagtaggaa gttcagcttc gagtggaagt
    33901 tatggtggcg gcatgactat ggaagatatt ttttcccact ttggcgatat ttttggcggt
    33961 cattttggcg gcttttcggg ttttggaggt ggatcgagaa gcagcgctcc ccgtatgcga
    34021 agaggaaccg atttgcgggt aaaagtcaaa ttaacattgg ctgaaatagc tgaaggagtc
    34081 gagaaaaaaa taaaagtcaa gaaatatgtt ccctgttctc actgtcaagg aaccggagca
    34141 gccgaaggtt cggcgcccgt aacctgttcg acgtgtcatg caaccggaag ggttacgcgc
    34201 gttcagcaaa ccattctcgg acaaatgcag actacttccg attgtccaac ctgtcatggg
    34261 gaaggaaaag tgattaagga aaaatgtatt cactgtaacg gagaaggaat tattcacgac
    34321 gaagaaatag ttaccatcaa cattcctgcg ggtgtggctg aaggaatgca gctaactttg
    34381 agcgggaagg gaaatgcggc tcgcagaggt ggaatcaatg gcgatttgat agtggtgata
    34441 gaggaagagc ctcattcgga actgattcgc gatggaaatg atttgattta caatttgttg
    34501 ctgagtgttc cgcaagcagc attgggtgga gctgttgaag ttccaaccgt taacggcaaa
    34561 gtgaaagtta acatcgatgc cggcacacaa ccgggtaaag tactgagatt aaggggcaaa
    34621 ggattacccg atgtcaacag atatggtaat ggcgatttgc ttattaatat tggcgtttat
    34681 atccccgaaa aattgagtaa ggaggagaaa accattatgg aaaaactgga acagtcgagt
    34741 aattttaaac ccagttcctc agctcaaaag ggttttttca gtcgttttaa agatatgttc
    34801 gattgaattc taaatttgta tttcatttcg aaaaacaggg gaaaaggaga gaaaaagtga
    34861 tttctttcct tttttggtaa gaaaataatt tgccgattct gccatagagt attaaccgaa
    34921 tcttaaattt cttaaatatc aacaagtata tccaattaaa aacaaattag attgtaaaat
    34981 ttttatttta taaatgcaat tatctacttc aacaataagc tttaataaac tttttacatg
    35041 ataattttcg ctgttctgtc acctgccaca ataatataga gttgtcccgg tattctgttt
    35101 tttaatgcat caatcgtaaa tttgttgtaa cgaggaattt ccgatacaat caatttccct
    35161 gtaacatcat atattcttac taccgtactg gtattcggaa ccgtaatggt taccgtttta
    35221 tcttctctgt tgacgtaagc tttcaattgt ttgccaacat caggcaaagt atgcacataa
    35281 atcaaatttg aaaaatcagt gatattttca taaagaatta ctgaatttct caatgttttg
    35341 tcgcttgcct tcactttata gtagcaatcg gaattggatg gtaaattaat aaccgtatcc
    35401 gttgttgagg taatccattt atccttttcg ttataattta ttttttttgc aaaccccacc
    35461 gtaatatcgt cgatgatcaa atatccgctt tccttttgat aagttaatcg aaattgatta
    35521 taatggtcag attcggtaaa ggtataagtt ttaatagcac tgagactggt agttactgaa
    35581 acattgtcaa tggttatcca tttgtctgag tcattttttg cttcaacctt aagtgctcca
    35641 ttcccagaaa aagatttgac gaagaaagat aattttatgg ccggcaatac ataattttcc
    35701 gtctgaatca tttcacctga gtttttaaat tgaatagccg gaacttcttt tccactgtat
    35761 gaagcggacg tactcaaagc ttgagcggta attgtccatc ccgatgatgg agtcaaacca
    35821 ttatcaaaac cttcagtcgt ttctgatgat tcattggaaa tataataggc agtgaaataa
    35881 tatcccgtgg catctgttac tggttcccaa tgagctacgt aactgctgat cgttacatcg
    35941 gtagcttcag tggcaacagg aggaataaca taaacaggtc ttgtggattt tccctgtaaa
    36001 ttcaaataga ttggagtggc atctttagaa gttatagaaa taatttcatt gtgaacatca
    36061 agataagacg gttcggttgg gttataacgt accaaaattc gggtagtatc cactacggaa
    36121 tcaataggag caaggattaa gtttttagtc cattctgcat taaagtcatt ttccaattgc
    36181 atttcgaaat gtttattttc tgaaaaagca agtagaatat tttcttttag tttttttccg
    36241 gaaattttta aaagttgtgc atttgatggc gttccctgaa cggtttcaaa agctgaaaaa
    36301 ttactcatag ccccaacaat cggagaattt tcttccccca tgaaatctaa atttatataa
    36361 ttgttttcag taatatttgt gagagatttt tttagtttcg taccatttct taaaaccaac
    36421 gaacaggttg taatgttatt tattcctgga aacggatctc cggcgagtgt ttgttcagaa
    36481 ccaataccgt cggcttcaac aattttataa cccagctttc cggcaatatt attgggtgta
    36541 ttgttttccc aatcgctttg attgaaattg atatgggtaa caagcaagcc gtgtccgggt
    36601 aaataagcgt cccaccctga cttttgcctg ttttccaaca taaaaaactc tgaaggattg
    36661 ggtgaatttc caaccaaatt atgattacca tcttttgaaa taagataggc gctgttggat
    36721 gaggttagag gttgcaatgt gaaatttccc gaacttttca gttcgatggg tgttaaccaa
    36781 ttcaggaaaa atcgatcgaa agccgaataa gtgggaggag ttttcccatc attcaaataa
    36841 gctccggcat ccatgatatt ccagtaagat aaggtaaaat gtgttcctcc atcggtagga
    36901 taataatcag ccagaccaag cacatgacca aattcatgac agaaagtacc gatgccgcac
    36961 atgtttgcgc ctctgcttga acgcaattca gaagaacagg cataatcgaa aacgacaaca
    37021 ccatcgaatt ttgtattgta attggccaaa gtccagcgat gtggccatat ggtattggct
    37081 ggtgctcctt ctgcttcatt gtagccggca taatagacaa aaatattatc tacgtatcca
    37141 tccctgtcgg tatcgtattg gctaaaatct acatcatcat tgtcggccaa tctacatgcg
    37201 tcaataatca tttgttgggg atttttatcc atgccatctg agccattttc accgtaatag
    37261 cccattgttt gtggcaaagt atagggccct accacatcaa attgggggtt aaactttccg
    37321 gttgaagtgt cgtaaaaata atcacgcgcc gagccggttg aactattttc ggaatatcca
    37381 ttttgattca aaagtttggt aaaagcttct tttggtgaac tgacagtaaa tgaattgtct
    37441 gaaaaattta ctaaaattac caatgatttc ggacttccgg tcaaaggata agctttacga
    37501 ccggattttt cggtaacagt aacagatgaa gctcgttgta tgcggttcaa ttgattttgt
    37561 ttcgaaaaat ctatcccttt gggaagttgt tttataaaat taatttcttc cttcgtacgt
    37621 ttttcgatat tgtttacgcg atatttagac ggagctattt cctttttttt acttaatttt
    37681 ccgtatttga aaaatccgtc cttgtccctg gtaatcaaat agccatctaa agtagtctga
    37741 taatgaaaaa attcgtctcc attcagttta acagaaattt ctgtaccgtc aggctgaacc
    37801 acctttaccg ggaaaggata ggcagggact gcttttgtaa attgtggaaa aacaaaacta
    37861 aaaacaaata
//
LOCUS       DAOSIB010000010        37458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_1267_length_37458_cov_10.494639, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000010 DAOSIB010000000
VERSION     DAOSIB010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 37458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37458
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1267_length_37458_cov_10.494639"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PLD44_01840"
     CDS             complement(<1..602)
                     /locus_tag="PLD44_01840"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOF98112.1"
                     /translation="MKSKFQNFSVKTNVHSEFKKGVFLFLIMSILSVSVSAQKGNNEV
                     RAGNKKYNNKKYIEAEIDYRKGLEKNPKSFEGNYNLGNSLFRQKKYEEAYQQYEKAAS
                     YAPKTEKEKLAATYHNMGNSLLMQKKIEESIEAYKQALRHNPKDNDTRYNLAYAQSLL
                     KKQQQQQNKNDKNKDQKQDQNQDNQQNKQNDNQKDQKNQQ"
     gene            complement(592..1626)
                     /locus_tag="PLD44_01845"
     CDS             complement(592..1626)
                     /locus_tag="PLD44_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOF98113.1"
                     /translation="MFRFAHPEYFFLLIVVPVLIGTFLYTQALKKRRLKKFGDPELLA
                     ELMPNVSYRRPVVKFFLQLFAVILLIIVLAQPQFGSKVENVKRQGIDVMIALDVSNSM
                     MAQDIQPNRLEKAKQILSKLIDQMNDDRIGLIVFAGDAYTQLPITVDYVSAKMFLSAI
                     STNMVPRQGTAIGTAIDMAIKSFGQKSQSGKAIIVITDGENHEDDAISAAKLAAQNGI
                     NVHVIGVGSPEGAPIPIPGTMSFKKDKDGNVVVSKLNEEMCKNIAAAGNGIYVRADNT
                     NNAYRIISKQLDTLAKSTTESKVFSDYSEQYQSFALIALIVLIIDFFLFDRKNKKLSK
                     IKIFDIENEK"
     gene            complement(1683..2666)
                     /locus_tag="PLD44_01850"
     CDS             complement(1683..2666)
                     /locus_tag="PLD44_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOF98114.1"
                     /translation="MTFHNPGYLFLLLLLLPIIYWYIKEMHKSDASLQISSLQDLTKF
                     PKSKRIKMRHLPFILRLFTLIFLIIAIARPQSSNSLRNETTEGIDIMIALDISGTMLA
                     EDLKPNRLEASKAVATEFILSRPNDNIGLVIFAAESFTQCPLTTDHDVLINLFKGVQY
                     GMIEDGTAIGLGLANAVNRIKDGKAKSKVIILLTDGSNNRGDIAPVSAAEIAKTFGIR
                     VYAIGVGSHGMVRVPVPTPLGMQYQLMESEFDEQTLKAIANITGGKYFRATDNLKLRS
                     IYQEIDQLEKTKISVREYSKKNEIFYIFALAAFVCLISEILLRNTVLRKIP"
     gene            complement(2719..3717)
                     /locus_tag="PLD44_01855"
     CDS             complement(2719..3717)
                     /locus_tag="PLD44_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HOF98115.1"
                     /translation="MRKRNKHIKLLINLCISLLVSTTLSAQKFSVTVKVDSSVIWIGD
                     QTGFTLEVSQAIGQKVNFPLFSDTLIHGLEIVEPLKIDTVAGDNNSIRVLHKYIITAF
                     IDTLYYIPSYPFVEGTDTVWSNSVSLKVVQPFQIDTASNSITDIKPVFQPKYDWSGLF
                     KKILLTLLVLAVLAAAYYFIRKKIQKKPVILIHKEEPAIPPHEAALHALDKIKEEKLW
                     MKGKYKEYHTQLTDVIREYIERRFLINSMEMTSSEILDNLPKNIYKEQKTAFSALHQL
                     LQLADLVKFAKWVPSPEENISSLNNAYLFVEQTKEETPLTEEKQTEKPVDKKEGEE"
     gene            complement(3736..4614)
                     /locus_tag="PLD44_01860"
     CDS             complement(3736..4614)
                     /locus_tag="PLD44_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOF98116.1"
                     /translation="METSEILKKVRKIEIKTRGLSQDIFAGAYHSAFKGRGMTFSEVR
                     EYQYGDDIRTIDWNVTARFGRPYIKIFEEERELTVVLLIDVSGSQEFGTVSRMKKDMM
                     TEIAATLAFSTIQNNDKIGVIFFSDKIEKFIPPKKGRKHVLYIIRELIDFRPESNKTN
                     LAGALRYFTNAIKKRSTAFVISDFMDSNFDNELTIANRKHDVVALQIYDERETEMPDI
                     GLVKLKDAETGQRIWIDTADKHLRTFYKHHWGERQLNLQRAFTKAKVDAVSMNTSDDY
                     VQALMRLFQMRSKIGR"
     gene            complement(4734..5729)
                     /locus_tag="PLD44_01865"
     CDS             complement(4734..5729)
                     /locus_tag="PLD44_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOF98117.1"
                     /translation="MDQSVDIRELNERIERESSFVDALVMGMNKVIVGQKHLIESLLI
                     GLLADGHILLEGVPGLAKTLAIKTLSDLIKGNFSRIQFTPDLLPADVVGTMIYSQKKE
                     EFSIRKGPIFANLVLADEINRAPAKVQSALLEAMQERQVTIGEKSFKLEEPFLVMATQ
                     NPIEQEGTYPLPEAQVDRFMLKVIINYPKKDEEKLIIRENLQKNMPSVQPILSPEDIV
                     KARDIVRQIYIDEKIEHYIVDIVFATRFPEEYGLESLKNMISFGASPRASINLALAAR
                     AYAFIKRRGYVIPEDVRAVCYDVMRHRIGLSYEAEANNMTSEEMITEILNTVEVP"
     gene            complement(5962..6879)
                     /locus_tag="PLD44_01870"
     CDS             complement(5962..6879)
                     /locus_tag="PLD44_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
                     /protein_id="HOF98118.1"
                     /translation="MNIKFHFLLFTAIWLSECLPFPLNAAKPQKETIKILAIGNSFSD
                     DAVEYLDELAIADSVPLIVGNLYIGGCSLQRHWKNATELLPDYSYRKSNLGIFTNQDH
                     KTLLDGLLDENWDIVVVQQVSQLSGKYDTYFPYLPNLMRFVKTHAKNPKVKFALHQVW
                     AYAQNSTHEGFANYDRDQMKMYLAITETVKKVAATTGIKIIIPSGTAIQNCRTSEIGD
                     HLNRDGYHLDLHIGRYVAACTWFETFFHRSCVGNPFKPAGVTDRQAKIAQYAAHYAVQ
                     RPFEITLMTNFAWTFLPSSEKTISEFLVY"
     gene            7356..9104
                     /locus_tag="PLD44_01875"
     CDS             7356..9104
                     /locus_tag="PLD44_01875"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOF98119.1"
                     /translation="MENDALLKEVMSKAQKWLDGNYDENTKNEIRRMMAAEDKTELIE
                     AFYKELEFGTGGLRGIMGVGTNRMNIYTVGSATQGLSNYLKAQFSDLPQISVVIGHDC
                     RNNSRHFAEISANIFSANGIKVYLFENLRPTPETSFAIRYLGCQSGIIITASHNPKEY
                     NGYKAYWNDGAQMVSPHDVNVIAEVNKITRVEDIKFEGNPELIEIIGEKIDKAFIDAI
                     KTLSLSPEAIRRHSNLKIVYTPLHGTGITLIPRALEEYGFKNIIHVPEQDVISGDFPT
                     VVSPNPEEPAAMEMAVNKAKEVDADIVMASDPDADRMGIAVKNDKGEWILVNGNQTAL
                     LFVYYLIRRWKELGKLTGNEYVVKTIVTTELIKNIADRNGVECFDCYTGFKWIASVIR
                     ENEGKKVYIGGGEESYGFLPEDFVRDKDAVSCCALMAEITAWAKDQGKSLFELLQDIY
                     LEYGYGKEKGISIVKKGKSGADEIQEMMRNFRNHPPKEIAGSKVSLIKDFQTLKMINL
                     KTNEENSLNMPATSNVLQFFTEDGTKISVRPSGTEPKIKFYIEVRGEMKSRADFDKAE
                     TEAMKKIEAVRASLGI"
     gene            9158..10147
                     /locus_tag="PLD44_01880"
     CDS             9158..10147
                     /locus_tag="PLD44_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567067.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOF98120.1"
                     /translation="MTLFYLLPFMQNFSRMGMDTGFQQRYLNYNQVLKSEFDERIQKI
                     SVHAGFTCPNRDGTKGWGGCTYCNNQTFVPEYCKPVKSITQQLDEGVNFFRHKYKSQY
                     YLAYFQSYTNTYDSFENLKSLYEEALHHPKIKGLVIGTRPDCVSEDLLDYFERISKNS
                     YVLIEYGIESTSDATLEFIRRGHDFACAEFAVKETARRGIKTGAHLILGLPFESKEII
                     LEHADKISRLPLTTLKLHQLQIVKGTVMTVQYRQHPEWFQLFTVDEFVDLVVDFLERL
                     SPNIAVERFVSQSPEGLVIAPQWKIKNFEITSKIEKQLIKRNTFQGRCWKGKD"
     gene            complement(10225..12645)
                     /locus_tag="PLD44_01885"
     CDS             complement(10225..12645)
                     /locus_tag="PLD44_01885"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOF98121.1"
                     /translation="MNFIKRYGLLIFLAIFAVSSPSQNLSGIKICVNPGHGGYDSDDR
                     NVVIAPFTSGDPNGFWESKSNLDKGLMLKSMLENAGATVIITRTTNTTADDLPLSQIV
                     AMANSANADYMLSIHSNAGNGIANYVLQLYAGLDPNDTYTYPTPTPFSDESRAISTIV
                     AQNLYSNQITNWSATYAVRGDKTFARTAMGWSDGYGVLRGLAVPGAISEGSMHDYIPE
                     TYRLMNMEYKWLEAWHFFKSFCQYYQAGNIPTGNIAGSVRDSRNIIQESYYKLKDTRD
                     ELLPLNGATLTLLETNETYTTDQLHNGVYMFKNLAPGVYHVKAEANGYHAQIFETTVT
                     ANNTTYLNIDLNKVRNTPPQVISYSPNIAPPDSAECSTDIVLTFNWDMDVTSTTEAFS
                     ITPSVEGTITFEDSQRILRFKPLLPLEKATLYTVRLAKTAKHPDNLSMTEDFTFQFLT
                     KNRNRLKLLAAYPYEGNHKVYTKPQFRFIFDRVLNTSNLQTAVKVLDKNGTELTKNAR
                     SVLNNKVQAPYGSTYFELTNALTPDEEYKVLIAGNVKDEIGMPLVEPIEINFKASNVL
                     VTDQPIVEKFETTNSYSYSNDQSLNVSSASVSRNTSKKLFEASSYLFSYSFSEPEAYA
                     TYLATTLGFVATHDKVVGLHVYGDLSGNSIELRFVAGAEVKYVKLCDLNFFGWEFVET
                     KLDHLQEGLPYEFTGFRVVRNEGILSGNGELYFDNMLLYDEPIMAIPQVLKNKILIYP
                     NPASNMVMVKTSDQFISQMQLYSLQGKLLKETNKTNLDISNVAAGTYVLKIKTEKDIV
                     SFPLIIVH"
     gene            complement(12660..13577)
                     /locus_tag="PLD44_01890"
     CDS             complement(12660..13577)
                     /locus_tag="PLD44_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98122.1"
                     /translation="MKTFKICLLGLFLIINSLSAQNVNIVKRYKVNGNDIKGSFPVLS
                     PAGDKLLFSSENHKGLWLYDLNSKELITICENEGAGYAPIFNADGTKVFYRKTIYQDK
                     RKFDAIESFDLVKKQKVEMISPQRNLSLLQNYHNGFLVSTDRKLLKATFGKTMNNIPP
                     YVCTEDLKLVVYANGKRTELNPIGENDARYIWVSLSPDSEKILFTVAGKGTFVSDLEG
                     KIVASLGYLNAPVWYDNNFVVGMQDKDDGHFITSSKVLMVSLNGKTKMTLSLDSEIAM
                     YPSASSKGNKVAYNKLEGDIIIVELNVKP"
     gene            complement(13658..16102)
                     /locus_tag="PLD44_01895"
     CDS             complement(13658..16102)
                     /locus_tag="PLD44_01895"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOF98123.1"
                     /translation="MNKKNFLFLLVSLVSFIGSAQTIDIDGKIYSIDTLANYQVGPGT
                     TYTGLRLKSNARLDVYFLKTAIHHPYVTIKTTLARDSIYGTETPSAMATRKSKEGSVY
                     FAGTNGDFFTTSGSYLGMPIGGCMADAELATMPVARKIFAFDENKSSYVGQMSYSGNL
                     KFHQSDFTIHHVNHLRATNELVLYNRNNGKYTHTNAYGTEVLVELLEGEHWKTNSSVK
                     AKVTKIEKNKGNMPIPAGQAVLSGHGTSQTILDNLSLNDTVEISIGLKLNNEIIAPFT
                     EMIGGDNRKEMLKDGVIETSEVWNELHPRTAIGYSQNKDTVIFCVVDGRGVSAGVTTK
                     QLAILMKSAGAYSAINLDGGGSSCMYVKYFGIMNSPSDGNERAVANGIFAVACAPTDN
                     VIAEIKSYTPIIQLPRYGVIMPKFLGYNQYGVLINTDLKGVTLSCTPDVGQILDDGRF
                     VATGSQGGILTAHYNNIETKLIIKLINSAEIAFRLDSVLVDQRSNYPIQVQTTIGINT
                     MDVLPAALSWTIRDPEICSINENGQLTGLKNGSTMVIGSLGDFKDSLKVTVQIPTSGR
                     MIGDNFNIENWTLNASTPLNAVLNTQNLPANWTHGAAVNYVYTSTRAPFIKLLRNAPM
                     FGLPDTLKLVVNVGDINISKALLSLRANNASSFVSKEFTVIPKNTDTELSVAMSELFD
                     ATDIAIYPVWFDNINFYLGTQTAGQAYTLALKEIVLCYKGIEISGLPSVLLSQYEVFP
                     NPLEGNTLNIRIKNNDAKLLQAQLFHLNGQCVASQQLNPMNGIATFKIGKLPAGNYFL
                     KIINKETSETINLLIK"
     gene            complement(16329..17759)
                     /locus_tag="PLD44_01900"
     CDS             complement(16329..17759)
                     /locus_tag="PLD44_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98124.1"
                     /translation="MKKQVLFGIALLCSAFLGNVRAANPLQDTTYNAMGPNGEYTLTN
                     KWLYSVTLGNYVAAGDKIGTSGFVRGMAVKDGKMLFIDREKKQITVVNGETGLKEDPI
                     ILASNIFTYIGRNKADTADSIYVAGTLPYNDIKVDNAGNVLIGNCITSSAGRFQVWKI
                     NLTDGSGTLIIDQANLATLFPASTIRFDAFGVWGDVNTNAIIMASNASAMEAYKWTIT
                     NGVLSDPVMIEIDNVTEGTYLTGLPNPGSAPQIFPLDDNYFYLDGNATYPTLIDKDGN
                     VIDGFYNNPSALKDSTTMKDKILTMNQGHNGIAEFQVGDEYFVVMAATNTAGTPPSSF
                     RLFKFADANKAFSGLDCLWTFPMKGMGGSSNAYRTGMPAVEVSGNTAKIYVYTGENGY
                     GMYEFRVTQSGKPEISLQPVTVSVNGNTLKLSETVANLSIYNITGQLIGKFANVSSVE
                     APGKGIFVVKATTLHGETAIQKVIVK"
     gene            complement(17832..20636)
                     /locus_tag="PLD44_01905"
     CDS             complement(17832..20636)
                     /locus_tag="PLD44_01905"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOF98125.1"
                     /translation="MKKSIHLFVLSFIFLICCPFAEAQSPKREMRAVWLATVWQLDWP
                     SITVPKATGSNESARQAAINKQKNELTVIFDSLKKANMNSVFFQVRGMCDAMYTSSYE
                     PWSAYISSERGANPGYDPLAFAIEEAHKRGLELHAWLNPYRYSSSVYSHGNTSIDYSI
                     THPEWLLDYGNYVKILNPGLPEVRERIKCIIGEIVNNYNVDGIVFDDYFYAYGGTPNN
                     LDAAAQALYKPANQTIGDWRRENVNKMIAGVYDTIQKVKPYVTFGVSPFGTWTTDQNV
                     AAQRGIPLPIGVGTTGNMYAEIYCDPVAWLEQGTVDYLSPQLYWTTYSAYPYGILAEW
                     WSKLTNRFGKHFYSSHSLSSMTASSAPAAVASKIKINNEEIPTSAFSTLELELYGAKK
                     STSVSAAPSAVNFLPSEIGLQIDFNRDYDINDAPGSVFYATSKFMNTSGFISYLRNQK
                     FIHPALRPAISWKTHTDLPLISNLTISGNTLSWTYENDNVCFAVYAVPNENRNLPGIF
                     TSSDYLLGVSYNKQFILPASVNENSHKIAVTGMDRFGNEGSPRIYGESLTPSVAAQLI
                     YPENNTETVIPCVFQWETVQEADSYIWQLAEDENFTNLVCSRQTKENSFFSGKQTNLK
                     DSTKYYWRVKTLKANAEEIWSEIRNFTSHKFKILSPTNGSTNVSLLPAFKWDSISPTA
                     SYTLEISSVWNFLESKRVFITTVKANSFVVPYGTLVGGTKYYARVTVNDGDISAVTES
                     ISFSTVEETIPVPQILLPTNGSTIQGNSIQVCWAEQPARGFRVELSTSSAFPPRSTTV
                     KTVDAYTFCTTYEQLTNNTYYIRVKASTPNGLTEASDTVAVTLKDNTSIGNNALMVNW
                     KGYFVKNGNNIHLMLDVPSTEKVSIKLYSITGTLLFQKEITLQEGINQIPISTEHLLQ
                     GIYPLEITGEKNRLILKLIY"
     gene            complement(20697..22367)
                     /locus_tag="PLD44_01910"
     CDS             complement(20697..22367)
                     /locus_tag="PLD44_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOF98126.1"
                     /translation="MKTKQIIKYLGIFGLLVFFTGSCKDEMPAIEEFPSPDVTFTYSV
                     NDNSYQLDYYVGAEIKFETTSATTGCTWDFGDGTNATGTTVIHKYTQAGTYQVKLTVE
                     GKGYVIQKIFISDIRPIMKLKPIEGGMCEVQTTYVSFEVELPNPEGLEEEYLWTFPEG
                     TLDENGNSRETYTGKDPGKVKFSNVGSQTVRLQVKLGGRQLEESSINVQVAFNRAVPT
                     LYYAVKTGNIMALKLSSSTPEGMKIYPFDMGVKSGQHPLNILFNDTSLYILDCGKQFT
                     YINDIDGNLGDGRITVMSKSGSKVETMLTNRGAAFDDPYYGYIESGKLYFSDRNTGFA
                     TISLTERNKSFSRSEFPYYVQNATLGYYGKGLSYGAMNACFGKINNVWYWCKTFNGNG
                     IFRFTDDDILKAPITGNGTPPAAGVALDGMSPKSFVWDSKNNVIYFSIFDQGYGGLYR
                     CTLEQLNAIKTFQDLTPYRCRLANGKMLQPVVEAGKGEGSNGEYIGICQLALDETDGC
                     VYFGYRSSDATIKSGLMRYNPAKGYVEPVIEGVEIYGVAINKTPSKLF"
     gene            complement(22456..23313)
                     /locus_tag="PLD44_01915"
     CDS             complement(22456..23313)
                     /locus_tag="PLD44_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98127.1"
                     /translation="MKNIQLKIAVLLSVFMGFFLTSCEKNDTLNDNVFIGEMAPQVYW
                     EINSSTVNAGSEVPFFVQYYTSGKEPIDRLEVWYSVEEEESKTVSCPWTQTFTFQLSS
                     TTSDNKRILQKISEYPHNESYWNDSLHAYSFNASFPTSSTLSSISWIKPATFDSTKMI
                     KYFGEGFMQHFKDSLYTKMKALDFQKMYSGLNLVENFKVFLDSTFNENSGSYDYHFQK
                     DSLGNEVIPTEIQNIYQTIPFADLIYNASNNNYDVEFSRSYQLNALIKAIDRKGTAGL
                     STKSIITLN"
     gene            complement(23338..25122)
                     /locus_tag="PLD44_01920"
     CDS             complement(23338..25122)
                     /locus_tag="PLD44_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOF98128.1"
                     /translation="MNTTIMKGNIFKVISLILLVSIGSLLSSCEDWLDINVSKDKPVK
                     VTPDQSLPLVLLYASQLCYDHAEYGVYLSQALTTAGRSQTGSYAYKSGWEFLSMNRHP
                     QWRRHFYDIGVNAKGMIETAEEIGSKNFVLIGRTILLHSTLLTTDAFGDMPRSQVYLS
                     NSPSYDTQASIYEWMLQEADELISFYNNDEWVNNPNNKRINKNMDRMFEGDLGKWRAF
                     AKALKARILIRKLPNWENTPETCQKIIDAVDAALNDPSYTDAIYKYDGGSAEKNCPWG
                     PAQPSLNLGWPQGRQNLLSEAIPSKFFTYAILGSYSSYRATTGYALDPRAVELMTPRS
                     NDNNVKVLRYLENNIGMPATMKITFYPDLYATEGHNPYTRNNGYIMLMSREELLFIKA
                     EAQYWKGDKTAAYQTTIDAVNVNMERLGVLPTSAATKPYYDRFFSIKLPSANTFTIAD
                     LMQQKYVAMYLQPEQWTDMRRYNYSSKTNGIAYDGQFVYTVTTCHDGSRYVNPDNFKV
                     EFSLTRPYNLYEAYWTTPDNYGTNAKFSPNAWITRLNYDPETEEKYNRKELQRLGAYK
                     NPDWLKKRMIWAYKNNDKVTAADQTEWK"
     gene            complement(25137..28271)
                     /locus_tag="PLD44_01925"
     CDS             complement(25137..28271)
                     /locus_tag="PLD44_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOF98129.1"
                     /translation="MKKILLLFAVLFLSLQVSLAQKVTVTGTVTSKEDGLPIIGASVL
                     EKGTTNGTITNYNGVYTISVPIGATLVFSYVGMDKVERKVTGEGKIDVIMNPTAIAID
                     EVIVTAMGVKAEKKKLNFAVQSVDAEAITDGRSPNFVNSLQGKISGVSVTSSGGSPNA
                     GSQILLRGISSINPSQNNEPLFILDGMVLSGGGSAAADINPSDIENVTVLKGAAASAL
                     YGQNAANGAIMITTKTAKAGKMSLSANASIQSDTPTRLLELQSIYGPGALGFYRPQES
                     GGYGGWGPLLDKDEPVYNNVKNYFQSGLYQKYDFSINGGSDKFQSYASVNYSRHDGIV
                     PKDYLDKMGLLLKGNYEINNKISANFMTNIVKNSYRGAGSISAIYSWPINDDITHYKN
                     SDGSIRYRYISIPKKENSPISPLWSRYMDYGLNSSTRNLLQGSLIYKPLKGLEITGRL
                     SLDQNNYMYDGYQVPRFDDSIILDNPPKESDYTNLDAYAQALKEYNDYYYSVPYFNEK
                     DYASVDKDLFGNYSYSQSKRELVTATLMGNYKIPLPKDFNLEVLAGTEIKTDNRISSS
                     INGRDFVIPGTYSISNVREIVGTNDVSLNHWEKRNAGVFGELRSDYKGLLNLSFTSRW
                     DWSSTIAYKYNPYFYPSVTGGIIFSEIFGLSNDWFSYGKLRGNWARVGKDVATPYLFD
                     RKYTQYPTLPDGGYGADPTMSVASNLQPEISDSWEIGLETRFFSSKTRLDVAYYSTTV
                     DNQIVTVRVSPASGYILQTRNEGNIKNHGVEISLDQDILKNKDLLWTGTLNFGFNRGK
                     VLSLPEDIVELQGTQYGDIFPTAYLNGSTTAISGKDYLRNEKGEIICTPDGYPKINPT
                     KSVLIGNREPDFLMGLTSTLKYKGISLSFLVDGRKGGDVMNVTGRSLWSSGQHKMLEF
                     YRGRQVVWYGVVEQADGTYVKNTTPIVLDNQTIINYYYNVSSNFIEDGSYIRLSYVTL
                     GYDFTNKLKNNRYIHGLRCSLTGTNLFLLTKYSGSDPQINASTSEGGTGSMGIDNYPV
                     PNTRGINFTINANF"
     gene            complement(28425..29249)
                     /gene="murQ"
                     /locus_tag="PLD44_01930"
     CDS             complement(28425..29249)
                     /gene="murQ"
                     /locus_tag="PLD44_01930"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOF98130.1"
                     /translation="MAFEKITEQNSLHNDLDKKDIRQLLVEMNEEDKKVPFAVERAIP
                     QIEQLIEKLVERMKIGGRLFYIGAGTSGRLGVLDASEIPPTFGMPPHLVIGVIAGGDT
                     ALRNAVEFAEDDMEQGWEDLKKHNISSIDTVVGITASGTAPYVMGALRKAKQHGILTA
                     SIACNPDSPVSHEADLKIELIVGPEFVTGSTRLKSGTAQKLTLNMISTVTMIKLGRVK
                     GNKMVNMQLTNSKLIDRGTRIIVEELNIDYEQARNILLIHGSVKKAIDAYKIQNKI"
     gene            complement(29262..30113)
                     /locus_tag="PLD44_01935"
     CDS             complement(29262..30113)
                     /locus_tag="PLD44_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOF98131.1"
                     /translation="MNIIADSGSTKTHWCVIEGENTVKEFYTKGINPFYQTKEEILAE
                     LKENLYPRIMQSTIDGVHFYGAGCAFAEQKKMLSEIFRDILGKELVVTIESDLLGAAR
                     SLFGKESGIACILGTGSNSCYYNGKGIEKNVPPLGFILGDEGSGAILGKKLTSDALKN
                     QLPPDLVSKFMKEYDLTLEKVLDHVYKKPFPNRYLAQFVPFIKENIQYPPLYNIVYSS
                     FNDFFNRNICQYPHSEKTPLGFVGSVAFHFADILQTIARERHFRIIDIAQNPMKGLIK
                     YHSQKNK"
     gene            30272..32254
                     /locus_tag="PLD44_01940"
     CDS             30272..32254
                     /locus_tag="PLD44_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOF98132.1"
                     /translation="MRTNLSSKITMTRIPRRYYQPDDVFELSRVTRLEKIPTEIFDTE
                     KEGSAKVAEEIAALIKTKQAAHEKCVLGLTSGKSMVGIYAELVRLHKKENLSFNNVVV
                     FLLFEYYPLTGKLKGSYGQLKNMLLDHVDIPEENIFYPDGTMDKHQIMQACAEYEEKI
                     ASFGGIDLQLLGIGRSGNVGFNEPGSLANSQTRLILLDTLSREDAAEFFSNVEMVPVS
                     AITLGISTILKAKRIVLVAWGEHKSTVVKNAVEGTMSEAIPASYLQLHSNVKIVIDLH
                     AASELTRISMPWLVTSCEWDSKLIRRAIVWLCMKLKKPILKLTNKDYNDNGLSELLAI
                     YGSAYNVNIRIFNDLQHTITGWPGGKPNADDSNRPERALPYPKRVLIFSPHPDDDVIS
                     MGGTFKRLIDQNHKVHVAYQTSGNIAVADHEVIRFLSFVKGFKDLFDSNNDVIQTKYE
                     AMKNFLLYEKKENDMDTPEILEVKALIRRGEAKAACRYLGLSSQNIHFLNLPFYETGT
                     VKKNDLSQKDIDIIKDLLQQIQPHQIYAAGDLADPHGTHKICLDAVLAAIDELKDEEW
                     MKDCRVWMYRGAWKEWEIDHIEMAVPLSPEELKNKRNAILRHQSQMESAPYLGNDERL
                     FWQRSEERNRATAELYTQLGLASYEAIEAFVRYKPV"
     gene            32296..33759
                     /locus_tag="PLD44_01945"
     CDS             32296..33759
                     /locus_tag="PLD44_01945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOF98133.1"
                     /translation="MKPINCFIVYDDAVQAEITIENLLQSELVNAVYLLSKRENIQVN
                     EKCFPILIDSLYSSNTIRKIAEKSDTPYSLVYLKNTPLHLGQFALERFFQIAQDTKAG
                     MVYSDYYQVSGGVRSKLPLIDYQTGSVRDDFNFGSVLFFNAEVLKQASAQMNENFLHA
                     GLYDARLRISQKAELVHIDEYLYSEVESDNRKSGEKLFDYVDPKNRQRQLEMEHACTE
                     HLKNIGAYLKPQFKTILFDENNFEMEASVIIPVKNRATTIEDAVRSALTQKTSFPFNV
                     IVVDNHSTDGTSEIVEKLAKEDSRVRHIIPQREDLGIGGCWNVAVHSPFCGKFSVQLD
                     SDDVYQDENTLQIMVNAFYEQNCGMVIGSYTITDFEMNVIPPGLIDHREWTPENGRNN
                     ALRINGLGAPRAFYTPLLRKIKVPDSNYGEDYALGLYFSREYQIGRIFQSVYLCRRWR
                     DNSDAELDIVTLNTYNHYKDKIRTIEIKARQQINKLS"
     gene            complement(33896..35230)
                     /gene="gdhA"
                     /locus_tag="PLD44_01950"
     CDS             complement(33896..35230)
                     /gene="gdhA"
                     /locus_tag="PLD44_01950"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOF98134.1"
                     /translation="MDIEKFMQVLSAKHPNENEYLQAVREVLTTINEVYSQHPEFEKA
                     KIAERLVEPDRIFTFKVPWVDDQGNVQVNIGYRVQFNNAIGPYKGGLRFHPSVNLSIM
                     KFLGFEQIFKNALTTLPLGGAKGGSDFNPKGKSNAEVMRFCQAFMTELWRYIGPETDV
                     PAGDIGVGAREIGYLYGMYRKLAHENTGVLTGKNLEFGGSLIRPEATGFGALYFVREM
                     LQRAGMDIRGKTVAISGFGNVAWGAALKASQMGAKVVSISGPDGYVYDENGISGEKID
                     YMLELRASCNDIVSPYAEKFGVPFIPNRRPWELKVDIALPCATQNELNEEDARNLIEN
                     KVWCVAEVSNMGCTPEAIDLFIKNKIMYAPGKAVNAGGVSVSGLEMTQNAMHLSWTAE
                     EVDKQLHQIMNKIHEQCVNFGKEEDGYINYLKGANVAGFIKVARAMLAQGIV"
     gene            35535..>37458
                     /locus_tag="PLD44_01955"
     CDS             35535..>37458
                     /locus_tag="PLD44_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOF98135.1"
                     /translation="MNTLSINKLYFKDTSFANLMTHRIYNILLYASKYDAFVLEEDGR
                     IDEQIFNEYTALNLRYPPRFTLVSNEEEANELLRTRTFELIISMPSGDSINPFEWAKD
                     VKKEYPEIPIVVLTPFSSSISQRLAHEDLSAIDYVFSWLGNSDILLAIIKLIEDRMNV
                     EQDVNSVGVQVILFVEDSIRWYSSIVPHLYKYVFKQSRSFMTEALNEHEQMLRMRGRP
                     KILLARSYEEAISIYNKYSQNMLGVITDVSFPEKNVKDKKAGLHLCSEIRRNDKFIPL
                     IVVSTEEENRQTAEELNAVFLNKMSKTLFMELREKITDNFWFGDLIFFNPQTGVEEAR
                     VHNLRSLQDCIFKVSDEALYYHVSRNNISKWLYSRAMFPLAEFLKNIQVEPGMSENMK
                     NVRQIIFDAIVHYRKIKNRGIVAVFQRDRFDMYSNFARIGEGSLGGKGRGLAFLDAMI
                     KRNESLDKFENVQIAIPRTVVLCTDIFSDFMEMNQLYPIALSDIPDEEILAHFLKARL
                     PQKLLADLYAFLGAVSKPIAVRSSSLLEDSHYQPFAGVYSTYMVPFDANSKTHTLQMI
                     TEAIKAVYASVFYRDSKTYMAATKNLIDEEKMAIVLQEICGSAYENRYYPSFSGVARS
                     LNYYPIGNEKPEDGIAN"
BASE COUNT        10857 a         7162 c         7316 g        12123 t
ORIGIN      
        1 tgctgctgat ttttttggtc tttttggttg tcgttctgtt tattttgctg attgtcctga
       61 ttctgatctt gtttttgatc tttgtttttg tcgtttttat tttgttgctg ctgttgtttt
      121 ttcaacaacg attgggcata agctaaattg tatctggtgt cgttgtcttt tggattgtgt
      181 ctcagagcct gtttataggc ttcaatactt tcttcaatct tcttctgcat caacagggaa
      241 tttcccatgt tgtggtaggt ggcagccagt ttttcctttt ctgtttttgg tgcatacgaa
      301 gcagcctttt cgtattgctg ataagcttct tcatattttt tttgccgaaa aagcgaattt
      361 cccaaattgt agtttccttc aaacgatttg ggattttttt caagtccttt tcggtaatcg
      421 atttcggcct cgatgtattt tttattgttg tattttttgt ttcctgctct cacctcgttg
      481 ttcccctttt gagctgaaac agaaaccgaa aggatactca ttatcaaaaa gagaaatact
      541 ccctttttaa attccgaatg tacatttgtt ttgacagaaa aattctgaaa tttacttttc
      601 attttcaata tcaaaaattt tgattttgct caatttttta tttttcctgt cgaagaggaa
      661 aaagtctatg attaatacta tcagtgctat caaagcaaat gactgatact gttcgctgta
      721 gtcggaaaaa acttttgatt cagtagtcga tttggcaagc gtgtcgagtt gttttgaaat
      781 aatccgatat gcattgtttg tattatcggc tctgacatat atcccgttgc cggctgcagc
      841 aatattttta cacatttctt cattcagttt ggagacgacc acatttccat ctttgtcttt
      901 tttgaaactc atggtaccgg gaattggaat tggcgcacct tcagggctgc cgacgccaat
      961 tacgtgtaca ttgataccgt tttgggcagc cagttttgca gcgctgatgg cgtcgtcttc
     1021 atgattttca ccatccgtaa taacaataat ggcttttccg ctttgcgatt tctgtccaaa
     1081 agatttaatc gccatgtcaa tagccgtgcc aatggctgtt ccctgtcttg gcaccatgtt
     1141 ggtcgatatg gcggaaagaa acatttttgc cgatacgtaa tcaaccgtta tgggcaattg
     1201 ggtataagcg tcgccggcaa aaacaatcag tcctatgcgg tcatcattca tttggtcaat
     1261 cagtttcgac aatatttgtt tggctttttc gagtcggttg ggttgaatgt cctgcgccat
     1321 catggagttt gaaacatcca atgcaatcat gacatcgatg ccctgccgtt taacattttc
     1381 cacttttgac ccgaattgtg gttgtgccaa aacgataatc aacaagatta cggcaaaaag
     1441 ctggagaaaa aatttgacaa ctggacgccg ataagacacg tttggcatca attcagccag
     1501 caattccggg tcgccgaatt ttttcaaacg tcgtttcttc aaagcctgtg tgtagagaaa
     1561 agtccctatc aacacaggaa caacaatcag cagaaaaaaa tattccggat gagcaaatcg
     1621 aaacataaaa tatctgatta atttttaatc tatttctttc atttttaatg caaaagattt
     1681 tgtcagggaa tttttcgcaa taccgtattt cttaacagta tttcagaaat caggcaaaca
     1741 aaggcagcca gcgcaaaaat gtaaaaaatc tcgtttttct tgctgtattc gcgaacactg
     1801 atttttgtct tttccaattg atctatttcc tggtatatac ttctaagttt caggttgtcg
     1861 gtggcacgaa aatatttacc tccggtaata ttagcaatag cttttaaagt ttgttcgtca
     1921 aattctgatt ccataagctg atattgcatg ccgaggggag taggaaccgg aactcttacc
     1981 attccgtgag aaccaacacc gatggcataa accctgatac cgaatgtttt tgcaatttcg
     2041 gcagccgaaa ccggagcaat gtcgcctcga ttgtttgagc cgtccgtcaa caaaataatg
     2101 actttcgatt tggcctttcc atcttttatc cgattcacgg cattggccaa cccaagtccg
     2161 atggctgttc catcttctat cattccgtac tgaacacctt taaaaaggtt gataagtacg
     2221 tcgtgatcgg tagtgagcgg acattgggta aaactttcgg ctgcaaaaat caccaatccg
     2281 atgttgtcgt tcgggcggga aagaataaat tccgtagcta cggcttttga tgcttccaat
     2341 cgattgggtt tcaaatcttc tgccagcatg gttcccgaaa tatcgagggc tatcattatg
     2401 tcgatgcctt ctgttgtttc gtttcgtaaa ctattggagg attgaggtcg ggcaatggca
     2461 ataatcagga atatgagggt aaataaacgc aaaataaaag gtaaatgcct catttttatc
     2521 cttttacttt tcggaaattt tgtcaaatct tgcaatgaag atatttgcag actcgcatcc
     2581 gacttgtgca tttccttaat gtaccaataa ataatcggta gcaacagcaa caagagaaat
     2641 aaatatcccg gattatgaaa agtcattttt tattgtaagc gttttttatc tgtttaacca
     2701 aatttttttg ctttatcttt actcttcgcc ttctttttta tccacaggct tttcggtttg
     2761 cttttcttct gtgagtggcg tttcttcttt ggtttgttcc acaaacaaat aggcattgtt
     2821 caaactcgaa atgttttctt cgggcgatgg aacccacttg gcaaatttca ccaaatcggc
     2881 aagctgtaaa agctgatgta aagctgagaa tgccgttttt tgttctttat aaatattttt
     2941 cggcaaatta tccaaaattt cgctggaagt catttccatg ctgttgatca ggaagcgtct
     3001 ttcgatgtat tcccgaatga catccgtcag ctgggtatgg tattctttgt attttccctt
     3061 catccaaagc ttttcttcct tgattttgtc gagagcatgg agagctgctt cgtgtggcgg
     3121 aatggccggt tcttccttgt gaatgagtat gacaggtttt ttctggattt tttttctgat
     3181 gaaataataa gccgctgcca gcacagcaag caccagcaat gtcaacagta tttttttgaa
     3241 taatcccgac cagtcatatt ttggctgaaa aacgggctta atgtctgtta tggaattgga
     3301 agccgtatcg atttgaaatg gctgcactac ctttaacgac acagaattgg accaaaccgt
     3361 atctgtacct tcaacaaaag gatacgaagg aatataatag agggtatcta taaaagccgt
     3421 aataatgtat ttgtgcaaaa cgcgaattga attgttgtct cctgcaaccg tatctatttt
     3481 tagcggttca acaatttcca acccgtgaat aagggtatcc gaaaagagcg gaaagttaac
     3541 tttttgccct atagcttgag atacttccaa cgtaaaacct gtttgatcac caatccaaat
     3601 gacggaagaa tcgactttaa cagtaactga aaatttttga gccgacaggg tagtggaaac
     3661 caataaactg atacacaagt ttatcagcag ctttatatgt ttattccttt ttcgcatttt
     3721 ttaaaaaatt ccgtattaac gtccaatttt acttctcatc tggaacagcc tcatgagcgc
     3781 ctgtacataa tcgtcggaag tattcatgga gacggcatct acctttgctt tggtaaatgc
     3841 acgctgcaag ttcagttgtc tttctcccca atggtgtttg taaaatgttc gtaaatgctt
     3901 gtcggccgta tcaatccaga tacgttgacc ggtttctgca tcctttagtt ttacgagtcc
     3961 gatgtccggc atttccgttt ctctttcgtc gtaaatctgc agtgcaacta catcatgttt
     4021 acggttggct atggtcagtt cattgtcaaa attgctgtcc atgaaatcgg aaatgacaaa
     4081 agcggtcgag cgttttttta tggcattggt aaaataccgc aaagctcctg cgagatttgt
     4141 tttgttgctt tcggggcgga aatcgatcag ttctctgatg atgtaaagta cgtgttttcg
     4201 tccttttttc ggaggaatga atttttctat tttgtcggag aaaaaaatca cgccaatttt
     4261 atcgttgttc tgaattgttg aaaaagccaa tgtagctgca atttctgtca tcatgtcctt
     4321 tttcatgcgc gaaactgttc caaattcctg actgccggaa acatcaatga gcaaaacaac
     4381 tgtcagttcg cgttcctctt caaaaatttt aatgtagggg cgtccaaaac gggcagtaac
     4441 attccagtcg atggtacgaa tatcgtcgcc atattgatat tcacgcactt cggaaaaggt
     4501 cattcctcga cctttaaaag cggaatgata agcgcccgca aaaatgtctt gagacaatcc
     4561 gcgggttttt atttcaattt ttcgaacttt ctttaaaatt tcactcgttt ccataaatta
     4621 acgtgtagat ggaaaatttt ctccactttt ttccgacagt tttagctgca tatttttctc
     4681 ttttctgtct ccggtggaac atttttaaac actcttaatg acaaagccgt tttttatgga
     4741 acttcaaccg tattcaaaat ttcggtaatc atttcttcgg aagtcatatt gttggcttcg
     4801 gcttcataac ttaaaccgat gcggtggcgc attacgtcgt agcaaacggc acgaacatct
     4861 tcgggaatga cgtatccgcg tcgtttgata aaggcataag cacgtgctgc caacgccaaa
     4921 ttgatggaag ctcttggtga tgcaccgaac gaaatcatat ttttgagcga ttccaatccg
     4981 tattcttccg ggaatcgggt agcaaacaca atatccacaa tataatgttc tattttctca
     5041 tcgatataga tttgtcgcac aatatcgcgc gcttttacaa tgtcttcggg gctcagtatg
     5101 ggttgaacgg aaggcatgtt tttttgcagg ttttcgcgaa taatcagttt ttcttcatct
     5161 tttttcggat aattgatgat gactttcagc atgaaacgat cgacttgcgc ttcgggaagc
     5221 ggatatgttc cttcctgttc gatgggattt tgtgtggcca tcaccaaaaa aggttcttcc
     5281 aatttgaaag atttttcgcc aatcgttacc tgccgttcct gcatggcttc gagcaaggca
     5341 ctttgcactt tggccggagc tctgttgatt tcgtccgcca aaacgaggtt ggcaaaaata
     5401 gggcctttac gaatgctgaa ttcttctttt ttctgactgt aaatcattgt tccgacaacg
     5461 tcggcaggca gcaggtcggg tgtaaactga attcggctga aatttccctt aatcaagtcc
     5521 gaaagtgttt tgatggccag cgttttggca aggccgggta ccccttccag taaaatatgc
     5581 ccgtcagcca gtaaaccgat aagcaatgat tcgatcaaat gtttttgtcc tacaattacc
     5641 ttgttcattc ccataacgag agcatccaca aatgagcttt cccgttcaat acgttcgtta
     5701 agttccctta tatcaaccga ttgatccata ttgttttttc tttgtttgtt tattgaattt
     5761 gttgagcaaa tatacagcta tttgaaacgt taaaaatctg atttaataac ttttaacctg
     5821 aatttagatg attaagtaat atccttctta gcgctatttt gcagtgaaaa atgctttatt
     5881 cttctcatag ttggcttatt gatatttttc acttttcaac tgtacagata gtggaaataa
     5941 taaattgaaa tttttcttaa cttaataaac caaaaattcc gaaattgttt tctctgaaga
     6001 tgggagaaaa gtccatgcaa aattggtcat caatgtaatt tcaaagggac gctgaacggc
     6061 atagtgagct gcatactgag ctatttttgc ctgtcggtcg gttacgccgg caggcttgaa
     6121 agggttaccg acacagcttc gatgaaaaaa ggtttcgaac catgtgcaag ctgcaacata
     6181 ccggccgata tgcaaatcga ggtgataacc gtcgcgattg agatggtcgc caatttcgct
     6241 ggttctgcaa ttctgaatgg ctgttccgga aggaataatt atttttatcc ccgtagtggc
     6301 tgcaaccttt ttcactgttt cggtgatggc cagatacatt ttcatttgat ccctgtcgta
     6361 attggcaaaa ccttcatggg tagaattttg tgcgtatgcc caaacctgat gcaatgcaaa
     6421 ttttactttt ggattcttgg cgtgtgtttt gacgaatctc atcaagttag gcagataagg
     6481 gaaataagta tcgtattttc ccgaaagttg gctaacctgc tgcactacca caatatccca
     6541 attttcgtcg agcagtccat ccaacagcgt tttgtgatcc tgattggtaa aaatgcccaa
     6601 attgcttttg cggtaagaat aatcgggaag gagttccgta gcatttttcc agtgcctttg
     6661 cagcgagcaa ccaccaatat acaaattgcc aacaatcagc ggaacgctgt cggctattgc
     6721 cagctcatcc aaatattcta cggcatcatc ggaaaaactg ttgccgatgg ccagaatttt
     6781 aatggtttcc ttttgcggtt tagcggcatt caatgggaaa ggcaggcatt cgctcagcca
     6841 aatagcggta aacaataaga agtgaaattt tatattcatg gtggtttggt gtttttcgtt
     6901 gatagtgaaa actttcttta ctgcaaaaat agcattttca tgcaaagcct ttgatggaaa
     6961 tagtcttgtt ttaatctaat ttaatggcca aagcggattt ttcacttttt tatagagaaa
     7021 agatggtcaa atttgaactt aacatcgaat ttatcgagaa aaatttgatt ttaattaccc
     7081 aatacttaaa aatgagttca tgaaattatt aatgcaattt cgacttgata gttgaatcta
     7141 ttagttctaa aaaacagtct attttttgac ctaagaccta aatttattat ttttgtcgga
     7201 gaaaagccga atttgtttga tattttaatc tgcaggtaat ttcctgattt aaaaatttca
     7261 tttgaaattt gatcgaaaca gaaacaaaaa acaagtgggg cagaagcgtt tttgaatttt
     7321 agaaattaaa aagttaacat caaaaatata aaaatatgga aaatgatgca ttattaaagg
     7381 aagtgatgtc gaaagcacaa aaatggctgg atggaaatta tgatgaaaat accaaaaatg
     7441 aaatccgcag aatgatggct gccgaagaca aaacagagtt aatagaagct ttctataaag
     7501 aattggaatt cggtaccggt ggtttgcgcg gcattatggg cgtggggacc aatcggatga
     7561 atatttatac cgtaggatca gccacgcaag gcttaagtaa ttatttaaaa gctcaattct
     7621 ccgatttacc tcagatcagt gttgtaattg gacacgattg ccgcaacaat agccgtcatt
     7681 ttgccgaaat ttcggcgaat attttttcgg caaacggcat taaggtttat ttgtttgaaa
     7741 acttaaggcc tacgcccgaa acttcttttg ccattcgtta tttgggttgt cagagtggaa
     7801 ttattattac agcttcgcac aatccgaaag aatataatgg ttacaaggcc tattggaacg
     7861 atggcgcaca gatggtttca cctcatgatg tgaacgtaat tgccgaagtc aataaaatca
     7921 ctcgggtgga agacataaaa tttgaaggca atcccgaatt gattgaaatc atcggagaaa
     7981 aaatcgacaa agcttttatc gacgcaatta aaactctttc cctgtcgcct gaggccattc
     8041 gtcggcatag caatttgaaa atcgtttata cgccacttca cggaacggga attaccctta
     8101 taccgagagc attggaagaa tacggtttta aaaatattat tcatgtgccg gaacaggatg
     8161 taattagtgg tgattttcct actgttgttt ctccaaatcc cgaagaaccg gctgcgatgg
     8221 aaatggctgt caacaaagcc aaagaagtag atgccgatat tgtgatggct tccgatccgg
     8281 atgccgacag aatgggaatt gccgtaaaaa acgacaaagg cgaatggata ttggtcaatg
     8341 gcaatcagac agctcttttg tttgtgtatt acctgattcg gcgctggaaa gaattgggaa
     8401 aactcacagg caacgaatat gtcgtaaaaa cgattgtaac aacagaactt atcaaaaata
     8461 ttgccgatcg gaacggagta gaatgtttcg actgctatac cggttttaaa tggattgctt
     8521 ccgtcattcg cgaaaatgaa ggcaaaaaag tttacattgg tggaggagag gaaagttacg
     8581 gtttcttgcc ggaagatttt gttcgcgaca aagatgccgt ttcctgctgc gctttgatgg
     8641 ctgaaattac tgcatgggca aaagatcagg gaaaatctct ttttgaactt ttgcaggata
     8701 tttacctgga atacggatac ggaaaagaaa aaggaatttc cattgttaaa aaaggaaaat
     8761 caggtgcgga tgaaattcag gaaatgatgc gcaacttcag aaatcatcct cccaaagaaa
     8821 ttgctggctc aaaggtttcc cttatcaaag attttcaaac tttgaaaatg ataaatctta
     8881 aaaccaatga agaaaactca ttgaatatgc ctgccacatc gaatgtattg cagtttttta
     8941 cggaagatgg caccaaaata tcggttcgtc cttcaggtac agagcctaaa atcaaatttt
     9001 atattgaagt caggggcgaa atgaaatctc gtgccgattt tgacaaggct gaaactgaag
     9061 ctatgaagaa aattgaagct gttcgcgctt cgttaggaat ttaacgatat tgatttttta
     9121 gaatattgaa agcgtcattc tagataatta ttaacgaatg acgctttttt accttttacc
     9181 tttcatgcaa aattttagca gaatgggaat ggataccggc tttcagcaac gatatttaaa
     9241 ctacaatcag gttttgaaat cagaatttga tgaacgcatt caaaaaattt ccgttcatgc
     9301 cggattcacc tgtcccaatc gagatgggac aaaagggtgg ggtggctgta cttactgcaa
     9361 caatcagacg tttgtacccg aatactgtaa accggtgaaa agtatcaccc aacaactcga
     9421 tgaaggagta aatttttttc ggcataaata caaaagtcaa tattatttag cttattttca
     9481 atcatataca aatacatacg atagttttga aaatctgaaa tcgctgtacg aagaagcttt
     9541 gcatcatcca aaaataaaag gtttggtaat cggcactcgt cccgattgtg tgagtgaaga
     9601 tttattggat tatttcgaaa gaatttctaa aaacagctat gttcttattg agtatggcat
     9661 tgaatcaact tcggatgcta cattggaatt tattcgtcgc gggcatgatt ttgcttgtgc
     9721 cgaatttgcg gtgaaggaaa ctgctcgcag aggcatcaaa accggtgctc atttgattct
     9781 gggacttcca tttgaaagta aggaaataat acttgaacat gccgataaaa tttcccgatt
     9841 gcctttgact acattgaaac ttcatcaact tcagatagtg aaaggaacag tgatgacggt
     9901 tcaatatcgt caacatcccg agtggtttca actttttact gttgacgaat ttgtcgattt
     9961 ggtggtagat tttctggaga gactttctcc aaacatcgct gtcgaacgat ttgtttctca
    10021 atcgcccgaa gggttggtga tagccccgca atggaaaata aaaaattttg aaataacctc
    10081 aaaaatagag aaacaactca tcaagcgaaa tacttttcag ggaagatgct ggaagggaaa
    10141 agattgaaaa aataggatta taatataaat atcagtgaag agtgtacata ataagtaagc
    10201 tcttcactga tatttgtgtt ttttttagtg aacgattatt aacggaaaac tgacgatgtc
    10261 tttctctgtc tttattttca gcacataagt tccggcagcg acgttgctga tatccaaatt
    10321 tgttttattt gtctctttca ataattttcc ttgcaaagaa taaagctgca tttgagaaat
    10381 gaattggtcg gaagttttaa ccattaccat gtttgaagca ggattcggat aaatcagtat
    10441 tttatttttc aatacttgcg gaatagccat tattggctca tcataaagaa gcatattgtc
    10501 aaaatacaat tctccattac cagacaaaat cccttcattt ctaactactc taaaacctgt
    10561 aaattcatat ggcagccctt cttgtaaatg gtctaattta gtttctacaa attcccaacc
    10621 aaagaaattc aaatcacaca acttaacata tttgacttct gcacctgcaa caaatcgaag
    10681 ttctatcgag tttcccgata aatctccata cacatgaagg ccgactacct tatcatgtgt
    10741 agcaacaaat ccaagtgttg ttgccaggta agttgcatag gcttctggtt ctgaaaaact
    10801 gtagctgaat aaataagagg aagcttcaaa aagtttttta gaagtgttac gagacacgga
    10861 agcagaggaa acatttaaac tttggtcatt tgaataagag taactatttg ttgtttcaaa
    10921 tttttcaaca ataggttgat ctgtaactaa aacattagaa gctttgaaat tgatttctat
    10981 tggttccacc aaaggcatgc caatttcatc ttttacattg cctgcaatca acactttata
    11041 ttcttcatct ggagttaagg cgttggttag ttcaaagtag gtagaaccat aaggagcttg
    11101 aactttatta tttaaaacgc tacgtgcatt tttagtcaat tcagttccat ttttgtctag
    11161 tacttttaca gcagtttgta agttgctggt atttaaaact ctatcgaata tgaatctgaa
    11221 ttgaggtttt gtataaactt tatgattacc ttcataggga taagcagcaa gtaattttaa
    11281 acgatttcta tttttggtta aaaactggaa agtaaaatct tcagtcatgc ttaaattgtc
    11341 ggggtgttta gcagtttttg ccaatcgaac agtgtaaagc gttgcttttt caagcgggag
    11401 caatggtttg aatcgcaaaa tgcgctgact gtcttcaaaa gtgatggttc cttctactga
    11461 cggcgtaatc gaaaaagctt ctgttgttga agttacatcc atatcccaat tgaaagtcaa
    11521 aacaatatcg gtagaacatt cggcactgtc gggaggagca atattaggag agtaagaaat
    11581 tacttgtggt ggagtatttc ttactttatt caaatctata ttcaaatatg tggtattatt
    11641 tgctgtaact gtagtttcaa atatttgagc atgataacca ttggcttccg cttttacatg
    11701 ataaactccc ggagcgagat ttttaaacat ataaactcca ttgtgaagtt gatctgttgt
    11761 ataagtttca tttgtttcca aaagtgttaa tgttgcacca tttagaggaa gcaactcatc
    11821 tctggtgtct ttcagcttat aatagctttc ctgaattata tttcgactgt cgcgaacgga
    11881 gccggcaatg tttcctgttg ggatgttccc tgcctgatag tactgacaaa aggatttaaa
    11941 gaaatgccag gcttcaagcc atttatattc catattcata agcctgtagg tttcaggaat
    12001 atagtcatgc atagatcctt cagaaatagc tcctggaaca gctaatccac gcagcacacc
    12061 atatccgtcg ctccaaccca tagctgtgcg tgcaaatgtt ttatcaccac gcactgcata
    12121 agttgccgac cagttggtta tctggtttga gtaaagattt tgtgcaacaa ttgtgctgat
    12181 ggctctgctt tcatccgaaa aaggtgtggg tgtaggatag gtataggtat cgttaggatc
    12241 gaggcccgca taaagttgta gaacatagtt tgcaatacca ttccctgcat tgctgtggat
    12301 agaaagcatg tagtctgcat ttgccgaatt tgccatagct acaatttgag ataatggtaa
    12361 atcgtctgct gtagtattgg tggttcgggt aataataacc gtcgcaccag cattttccaa
    12421 cattgattta agcattaatc ctttgtccaa attgcttttc gattcccaaa atccgttcgg
    12481 atcgcctgaa gtgaaaggag caataactac atttctgtca tcgctgtcgt aaccgccatg
    12541 accgggatta acacatattt ttatacctga taaattttgg gatggggaag aaacggcaaa
    12601 aatcgctaaa aaaattagca agccatacct tttgataaaa ttcatgattt tttactgatt
    12661 tatggtttta cattcaattc cactattatg atatctcctt ccaatttatt gtaggcaact
    12721 ttatttcctt tagaagaagc tgaaggatac atggcaattt cgctatcgag gctcaaggtc
    12781 attttagttt taccgttcag cgaaaccatt agcactttcg aggaagttat aaaatgtcca
    12841 tcatctttgt cctgcatgcc cactacaaag ttattgtcat accaaaccgg cgcattcagg
    12901 tatccgagcg aagcaacgat ttttccttcc aaatcggata caaaagtccc ttttcctgcg
    12961 acggtaaaga gaattttttc gctatcggga gagagtgaaa cccaaatgta tctggcatcg
    13021 ttttctccaa tcggatttaa ttccgttctt tttccattgg cataaactac cagtttcaag
    13081 tcttccgtac aaacataggg aggtatatta ttcattgttt taccaaaagt agctttcaat
    13141 agttttcggt cggtagatac caaaaatccg ttgtgataat tttgcagaag agacaaattt
    13201 ctttgtggtg aaatcatttc gactttttgt ttttttacta aatcaaaact ttcgatggca
    13261 tcaaattttc gtttgtcctg ataaatagtc tttctgtaaa aaacttttgt accatcagca
    13321 ttaaatatcg gagcataacc tgctccttca ttttcacaaa tagtaatcaa ttctttagag
    13381 tttaagtcgt acagccacaa tcctttgtga ttttccgatg agaacaagag cttatctccg
    13441 gcagggctta ataccggaaa cgaacctttg atgtcgtttc cattcacttt gtagcgcttc
    13501 acaatgttta cattttgagc agataatgag tttattatca aaaatagccc cagtaaacag
    13561 attttgaatg ttttcattgg attctgtttt aaaatgatta taatagtgaa ttgaaatatt
    13621 tgaaattgtt aaatttatta tgatagtgaa taagattcta tttgatcaga agatttattg
    13681 tttctgaagt ttctttatta ataattttca agaaataatt gcccgcaggt aacttcccaa
    13741 tcttgaaagt agctattcca ttcattggat ttagttgttg agaagccaca cattgaccat
    13801 ttaaatgaaa gagttgagct tgcagaagtt ttgcgtcatt attttttatt cttatgttca
    13861 acgtattccc ttccagtgga tttggaaaga cttcgtattg agaaagcaat acagaaggca
    13921 gccctgaaat ttctattcct ttatagcaaa gaacaatttc tttgagagcc agcgtataag
    13981 cttgtcctgc agtttgtgta ccgagataga agttaatgtt gtcgaaccag acgggataaa
    14041 tggcaatatc tgtagcgtcg aaaagttcgc tcatggctac tgaaagttca gtgtcagtat
    14101 tttttggaat aacagtaaat tctttcgata caaatgaaga agcgttgtta gctctgagag
    14161 acaaaagagc ttttgagata tttatatctc caacattgac aaccagtttt aatgtatctg
    14221 gtaatccgaa catgggagca tttcttagaa gtttgataaa aggagctctt gttgaagtat
    14281 atacataatt gactgcagct ccatgcgtcc agtttgcagg caagttttgc gtattgagta
    14341 ctgcatttaa aggagtagaa gcattcaatg tccagttttc tatattgaag ttatcgccaa
    14401 tcattcttcc tgaagttgga atttgaactg ttaccttgag agagtcttta aaatcgccca
    14461 aactgccgat gaccatagtg ctgccatttt tcaaccctgt caattgtcca ttttcgttga
    14521 tggaacaaat ttcaggatca cgaatggtcc agctcaaggc agcaggaaga acatccatcg
    14581 tatttattcc aatagttgtt tgaacctgaa tgggataatt gctgcgttga tctaccaaaa
    14641 ccgaatccag tcgaaatgct atttcagctg agtttattaa ttttataatt aattttgttt
    14701 cgatattgtt ataatgagcc gttaaaattc ctccttgaga accggttgca acaaatcttc
    14761 catcatccaa aatttgaccg acatccggcg tgcaactcaa ggttactcct ttcaagtcgg
    14821 tatttatcag aacgccatat tgattatatc ccagaaattt gggcataatt actccatatc
    14881 ggggcaactg aataatcggc gtgtaggatt tgatttctgc aattacatta tcggttgggg
    14941 cacaggctac ggcaaaaatt ccattggcaa ccgcacgttc gttgccgtcg ctgggagagt
    15001 tcataatacc gaagtatttg acatacatgc aacttgaacc accgccatcg agattaatgg
    15061 cagagtaagc gcccgcactt ttcattaaaa tggccagttg tttggttgta actccggcag
    15121 aaacgccacg accatctaca acacaaaaaa tcacggtgtc tttattttgt gaatatccta
    15181 tcgccgttcg aggatgaagt tcattccaaa cttctgaagt ttcaattacg ccatctttta
    15241 acatttcttt tcgattatct ccgccaatca tttctgtgaa tggtgcaatt atttcgttat
    15301 ttaatttcag gccaatagaa atttccactg tatcattcaa agacagatta tccaatattg
    15361 tctgggaggt accatgcccg gatagaacgg cttgccctgc tggaataggc atatttcctt
    15421 tgttcttttc aattttggta acttttgctt ttactgagct gttagttttc caatgttctc
    15481 cttcgagcaa ttcaaccaaa acttctgttc cataagcatt ggtatgagta tattttccat
    15541 tgtttctatt gtaaagaacc aattcattag tagcgcgtaa atgattcaca tgatgaatcg
    15601 taaaatctga ctgatgaaat tttaaatttc cactataact catttgacct acataagatg
    15661 atttattttc atcaaaagca aatatttttc tggccacagg catggtggcc aattcggcat
    15721 ctgccatgca tccgccaatc ggcatgccaa gatagctgcc tgaagtggta aaaaagtcgc
    15781 cattggttcc ggcaaaatac actgagcctt ctttactttt gcgggtagcc atagcagaag
    15841 gagtttctgt tccataaatg gaatcgcggg ccaaagtggt ttttatcgtt acatagggat
    15901 gatggatagc tgtttttaaa aaatatacat ccaaacgcgc attgcttttt agtcgcaggc
    15961 cggtataagt agtaccggga cccacttggt aattggccag tgtatcaata ctgtatattt
    16021 tcccgtcaat atcaatggtt tgtgccgaac caataaaaga aacgagactc acaagaagga
    16081 aaagaaagtt ttttttattc atggctgttg acttttctga taattttcta agaaatcaaa
    16141 ataaggtatg caaatacgaa tgaatttccg agtgaaaaat gaggatctta ttttttgaga
    16201 aaacagaccg attgaaattt tttgtcaagg caggtataga aacaatttaa gtagaatata
    16261 actgaaaatc tgaaattcaa acatgtggcg aatttatcgc cacatgtttg aaaaaaatga
    16321 aagaagaatt atttaacaat aactttctgt atggctgttt caccatgtaa tgtagttgct
    16381 tttacaacaa atattccttt acccggagct tcaacggaag aaacattggc aaactttcca
    16441 attaactggc cggtaatgtt atagatgcta aggttagcta ccgtttccga aagttttaac
    16501 gtgtttccat taactgatac ggttactggt tgcagcgata tttccggttt ccccgactgt
    16561 gtaactctaa attcatacat tccgtatcca ttttcaccgg tgtaaacata aatctttgcg
    16621 gtattgccgc ttacttcaac tgctggcatt cctgtacgat aagcatttga tgagccaccc
    16681 atgcctttca ttggaaaggt ccacaagcag tccaaacccg agaaagcttt gttggcatcg
    16741 gcaaatttaa acaaacggaa tgatgatgga ggtgttccgg cggtattggt agcagccatt
    16801 actacaaaat attcatctcc aacctgaaat tctgcaattc cgttgtgtcc ctgattcatg
    16861 gtcagtattt tatccttcat ggtagtagaa tcttttaaag cagaaggatt attgtaaaat
    16921 ccgtcaatta catttccatc cttgtctatt aacgtgggat aagttgcatt tccgtccaaa
    16981 tagaaataat tatcgtccaa tgggaaaatt tgaggagctg agccagggtt aggcaatcct
    17041 gtcaaatagg taccttcagt aacattatca atttcaatca ttacggggtc agataaaact
    17101 ccgttggtaa tggtccattt gtaagcctcc atagctgaag catttgaggc catgattatt
    17161 gcattggtat tgacatcacc ccatactccg aaagcatcaa aacgaattgt agatgccggg
    17221 aacaaagttg ccaagtttgc ctgatcaatg ataagtgtac ctgaaccatc tgtcagattt
    17281 attttccaaa cttggaaacg acctgccgat gaggttatgc aattgcctat gagtacattg
    17341 ccggcattat cgacttttat gtcgttgtat ggaagagtcc ctgcaacata tatactgtca
    17401 gcagtatcgg ctttgtttct accaatgtag gtaaaaatgt tgcttgccaa aattatcgga
    17461 tcttccttca aaccggtttc gccgtttact acggtaattt gtttcttttc ccgatcgatg
    17521 aaaagcattt tgccatcttt tacagccatt ccgcgaacaa atccggatgt accgattttg
    17581 tcgccagcag caacataatt tcccagcgtt accgaataca gccacttgtt ggtgagggtg
    17641 tattctccgt ttggtcccat tgcattgtaa gtggtgtcct gcagcggatt cgctgctctt
    17701 acattgccca ggaatgcact gcatagcaat gctattccaa aaagtacttg ttttttcata
    17761 atctaataaa atttaaaagt tagtaaatat aaaattaata aataattaga aattcccttt
    17821 tttgtcaaaa gttaataaat cagtttcagt atcagtctgt ttttttctcc ggttatttcc
    17881 aatggataaa taccttgcag caggtgttct gtagatattg gaatttggtt aattccttcc
    17941 tgcaaagtta tttccttttg gaagagtaaa gttcccgtga tggaatacaa ttttatcgaa
    18001 actttttctg tagaaggtac atccagcatt aaatgaatgt tgttgccatt tttcacgaaa
    18061 tatcctttcc aatttaccat aagggcattg ttgccgatcg atgtattgtc tttcagtgtt
    18121 accgcaacag tatccgaagc ttcggtcaac ccattcgggg tggaagcttt cacacggata
    18181 taatatgtgt tattggtcag ttgttcataa gttgtacaaa aagtataggc atcaacggtt
    18241 tttacggttg tgctacgtgg aggaaaagcc gatgaggtag ataattctac tctgaatcct
    18301 ctggcaggtt gttcagccca gcaaacttgt atggaatttc cctgaatggt actgccattg
    18361 gttggtagca atatctgagg tacaggaatg gtttcttcta cagtagaaaa acttatagat
    18421 tccgttacag ccgaaatatc cccatcgttt accgttaccc gtgcataata ttttgttcca
    18481 ccaaccaatg tcccataagg aacaacaaaa ctgtttgcct tcactgttgt gatgaaaacc
    18541 cgtttggatt ccaaaaaatt ccatacggaa gaaatttcaa gtgtatagga agcggttggg
    18601 ctaatggagt cccatttaaa agcaggcaaa agtgaaacat ttgtgcttcc gtttgttgga
    18661 gaaagtattt taaacttatg actggtgaaa tttcgaattt cagaccagat ttcttctgca
    18721 ttggccttta aggttttcac tcgccagtag tattttgtgg agtcttttaa atttgtttgt
    18781 tttcctgaga agaaactatt ttcttttgtt tgcctcgaac aaaccaaatt cgtaaaattt
    18841 tcatcttctg ccagttgcca gatgtaactg tcagcttcct gaaccgtttc ccattgaaat
    18901 acacaaggaa tgactgtttc agtgttattt tccggataaa taagttgagc tgcaacagaa
    18961 ggcgtaagcg attctccata aattctgggt gaaccttcgt ttccaaatct gtccatcccc
    19021 gttacagcaa ttttatggga gttttcattt acacttgccg gtaaaatgaa ttgtttgttg
    19081 taactcacac caagtaagta atcagaggaa gtgaaaattc cgggtaaatt tctgttttcg
    19141 ttgggtactg cgtaaacggc aaaacagaca ttatcatttt cataagtcca cgacaaagta
    19201 tttcccgaaa ttgtcaaatt gcttatcaaa ggaagatcag tatgtgtttt ccagctgata
    19261 gccggacgca aagccggatg aataaatttt tgatttctca aataagatat aaatcctgag
    19321 gtattcatga atttgctggt agcataaaaa acgcttcctg gagcatcgtt gatatcataa
    19381 tctctgttga aatcaatttg aaggcctatt tcggaaggca gaaaattgac cgcacttgga
    19441 gctgcgctaa cagacgttga ttttttagcc ccatacaatt ccaattcaag agtagaaaat
    19501 gcagaggtag gaatttcttc gttgtttatt tttattttgc ttgctacagc tgcaggcgcc
    19561 gacgaagcag tcatcgaaga aagtgaatga ctggaataaa aatgtttacc gaagcgattg
    19621 gtcagtttag accaccattc ggccaaaatt ccataaggat aggcactgta agtggtccaa
    19681 taaagttggg gagagagata atctactgtt ccctgttcga gccatgctac gggatcgcaa
    19741 tatatttcgg catacatatt ccctgtcgtt ccgactccga ttggcagagg aattcctctt
    19801 tgagctgcca cattttggtc ggtagtccaa gttccaaaag gtgaaacgcc aaaagtaaca
    19861 taaggtttca ctttttgaat agtatcgtaa actccggcaa tcattttgtt gacattttct
    19921 cttcgccaat cgccaatagt ctgatttgca ggcttatata aagcttgtgc tgctgcatcc
    19981 aaattgttag gtgtacctcc ataagcatag aaatagtcat caaaaactat tccatctaca
    20041 ttatagttat taacaatttc acctatgatg cattttattc tttcccttac ttcgggtaat
    20101 ccaggattta gaattttaac ataatttccg tagtcgagta accattcggg atgagtaatg
    20161 gaataatcga tggaagtgtt gccatgagaa tataccgaag aagaatatcg ataaggattt
    20221 aaccacgcat ggagttccaa tccgcgttta tgggcttctt caatggcaaa ggccaatgga
    20281 tcataaccgg gattggctcc gcgttcgctc gaaatataag cactccaggg ttcgtaggaa
    20341 gaagtataca tggcgtcgca cattcctcta acttgaaaaa aaacggaatt catatttgct
    20401 ttcttcagtg aatcgaaaat cactgtcaat tcattttttt gtttattgat ggcggcttga
    20461 cgtgccgatt cattactgcc cgttgctttg ggaaccgtaa tgcttggcca atcgagttgc
    20521 cataccgtag ccagccatac agctcgcatc tcgcgtttgg gagattgtgc ttcggcgaaa
    20581 gggcaacaaa tcaaaaaaat gaaagataaa acaaatagat gaatgctttt tttcatgcct
    20641 atatttcttt ttttaaagtt attttcaact ttcaaaattt cttttaaatt tccgaattag
    20701 aataatttcg atggtgtttt attaatggct actccgtaaa tttccacacc ttcaatgacg
    20761 ggttcaacat atcctttggc cggattgtat cgcattaagc ctgatttaat ggttgcatca
    20821 cttgagcgat aaccaaaata tacacatcca tcggtttcgt cgagtgccaa ttgacaaatg
    20881 ccgatatatt caccatttga accttctcct ttccctgctt ccaccaccgg ttgaagcatt
    20941 ttcccattag ccagcctgca acgatagggt gttaaatcct gaaatgtttt gatggcattc
    21001 aattgttcaa gtgtacatct gtaaagtcct ccataaccct gatcgaaaat ggaaaaatag
    21061 ataacgttgt ttttactgtc ccaaacaaac gattttggcg acattccatc caatgcaacc
    21121 cctgctgcag gcggcgttcc attgccggta attggagctt ttaaaatatc atcatccgta
    21181 aaacgaaaaa ttccgtttcc attgaaagtt ttgcaccaat accagacatt gttgattttt
    21241 ccgaaacagg cattcatagc tccataactt aatcctttgc cataatagcc cagcgtggca
    21301 ttttgaacgt aatagggaaa ttcgctgcgc gaaaaagact tattgcgttc agtaagcgaa
    21361 atggttgcaa atccggtgtt tctgtcagaa aaatataatt ttcctgattc gatatatccg
    21421 taataagggt cgtcgaaagc tgctcccctg ttggttaaca tggtttctac cttacttccg
    21481 cttttcgaca tgactgtgat tcgtccatcg cccaagttac catcaatatc gttgatgtat
    21541 gtaaattgct ttccgcaatc aagtatgtac aaagaagtgt cgttaaataa aatattcagc
    21601 ggatgttgtc ccgattttac acccatgtcg aaaggataaa ttttcatgcc ttccggagta
    21661 gaagaactta atttcagcgc catgatatta cctgttttta cggcataata aagcgttgga
    21721 actgctctgt tgaaggcaac ctgaacatta atgctgcttt cttcgagttg gcgaccgcct
    21781 aattttactt gcaaccgaac agtttgagaa ccaacattgc tgaattttac ttttcccgga
    21841 tctttgcctg tataagtttc tctggaattt ccattttcat caagcgttcc ttccggaaaa
    21901 gtccaaaggt attcttcctc aagaccttcg ggattgggta attccacttc aaaacttaca
    21961 taggtagttt gaacttcgca cattcctcct tctatgggtt tcaacttcat aataggacga
    22021 atatcgctga taaatatttt ttgaatcacg tatcctttgc cttctacagt cagtttaacc
    22081 tgatatgttc cggcctgagt atatttatgt attactgttg tgccggtagc atttgtgcca
    22141 tctccaaaat cccatgtaca accagtagta gcagaagtgg tttcaaattt tatttctgcc
    22201 ccaacataat aatctaattg ataggaatta tcattcaccg aataagtgaa agttacatcc
    22261 ggtgaaggga attcttcaat tgccggcatt tcgtccttgc aacttccggt aaaaaagaca
    22321 agcaaaccga atatgcccag atattttatt atttgtttcg ttttcataag cgaattattt
    22381 aatagtttca cgaattatga atgttcccgc gattgaattg ataaagtatg agatacattc
    22441 catgctttca attggttaat taagtgttat aatcgatttg gttgacaatc ccgctgttcc
    22501 ttttctgtcg atcgctttta tgagcgcatt caattgataa ctgcgtgaaa attcaacatc
    22561 gtaattgttg ttggaagcat tataaattaa atcggcaaaa ggaatagtct gataaatatt
    22621 ttgaatttcg gttggaataa cttcattccc gagagaatct ttctggaaat gataatcgta
    22681 actgccggaa ttctcattaa aagttgaatc caaaaacact ttgaagtttt ctaccaaatt
    22741 cagccctgaa tacatttttt gaaaatcgag tgctttcatt tttgtgtaga ggctgtcttt
    22801 gaaatgttgc ataaatcctt caccaaaata ttttatcatt ttggtgctgt cgaaagttgc
    22861 aggtttgatc catgaaatag aacttaatgt tgaactagta ggaaaagagg cattgaaaga
    22921 ataagcatgc aaagaatcgt tccagtaact ttcgttgtga ggatattcgg agattttttg
    22981 caatatgcgc ttgttatccg aagttgttga agaaagctga aaagtgaagg tttgagtcca
    23041 cggacacgaa actgtcttgg actcttcttc ttctacgctg taccatacct caaggcggtc
    23101 aatcggttct tttcctgaag tataatactg cacaaaaaac ggaacctcac tgcctgcatt
    23161 taccgtgctg ctattgattt cccaataaac ttgcggagcc atttcgccaa taaatacgtt
    23221 atcgtttaat gtgtcatttt tttcacagga agtcagaaaa aagcccatga atactgacaa
    23281 cagaacagct atttttaatt gtatgttttt catactcgta atttttaaag tttaaattta
    23341 tttccactct gtttggtctg cggcagtaac cttgtcgttg ttcttgtatg cccaaatcat
    23401 tctttttttc aaccaatccg gattcttata agcgccaagt ctttgtaatt ctttgcggtt
    23461 atatttttct tccgtttccg ggtcgtaatt caagcgggta atccatgcat tgggcgagaa
    23521 tttggcattg gtgccgtaat tatcaggtgt tgtccaatag gcttcgtaga gattgtaagg
    23581 tctggttaaa gaaaattcca ccttgaaatt atcggggtta acgtaccttg aaccatcatg
    23641 acaggttgta acagtataaa caaattgacc atcataagct atgccattgg ttttgctgct
    23701 gtagttgtat cggcgcatgt cggtccattg ttcgggttgc aggtacatgg ccacatactt
    23761 ttgctgcatc aagtcggcaa tggtaaaggt attggcactt ggtagcttga tagaaaagaa
    23821 acgatcgtaa taaggctttg tagcagcact ggtaggtaat acacccaaac gttccatgtt
    23881 cacatttacc gcatcaattg ttgtttgata ggcagcagtt ttatcacctt tccaatattg
    23941 ggcttctgct ttgataaaca gcagttcttc acgcgacata agcataatgt aaccgttatt
    24001 ccgtgtgtag ggattatgtc cttcagtagc atataaatct gggtagaaag taattttcat
    24061 ggttgccggc atgccaatgt tattttcgag atagcgtaaa acttttacgt tgttatcgtt
    24121 tgaacgaggg gtcatcagct ccacagcacg ggggtcgaga gcgtaaccag ttgtagccct
    24181 gtaacttgag tagcttccaa gtatggcata agtaaaaaat ttggaaggaa tcgcttcaga
    24241 aagcagattt tgtcgtccct gtggccaacc caaattcaag gatggttgag ccggacccca
    24301 cggacaattt ttttcggccg aacccccatc atacttgtag atggcatcgg tataggaagg
    24361 atcatttaat gctgcatcta ctgcatcgat aattttttga caagtttcag gggtgttttc
    24421 ccagttggga agttttctga tcagtattct tgcttttagg gcctttgcga atgccctcca
    24481 ttttcccaag tcgccttcaa acatgcggtc catgttttta ttaatgcgct tgttgtttgg
    24541 attgttcacc cattcgtcat tattataaaa gcttatcagt tcatctgctt cctgcagcat
    24601 ccattcgtat atggaagctt gtgtgtcgta cgaaggtgaa ttggacaggt aaacctgtga
    24661 acgtggcata tcgccaaaag catcggtagt tagcaaggtt gaatgcaaaa gaatggtacg
    24721 cccaatcaat acaaagtttt ttgaaccgat ttcttcagca gtttcaatca ttcctttggc
    24781 gttgacgcca atatcgtaaa aatgccttct ccactgagga tgacggttca tcgataaaaa
    24841 ttcccatcca cttttgtagg cgtacgaccc tgtttgactg cgtcctgcag tagtaagtgc
    24901 ctgtgataga tatactccgt attcggcatg gtcatagcaa agttgggatg catacagcag
    24961 aaccagcggc aacgattggt cgggagtaac tttcaccggt ttgtctttgc tgacgttaat
    25021 atccagccag tcttcgcaac tgctaagcag gctgccgatg gaaacgagca gtataaggct
    25081 gataaccttg aaaatgtttc ctttcataat tgtggtattc attgttgtat tgagatttaa
    25141 aagttggcat taattgtaaa gttgattcct ctggtattgg ggacaggata gttgtctatt
    25201 cccatcgagc cggttcctcc ttcgctagta gaagcattga tttgaggatc ggaaccggaa
    25261 tatttggtta aaagaaataa attggtaccg gttaaggaac atctaagtcc gtgaatatag
    25321 cgattgtttt ttaacttgtt ggtaaaatca tatcccagtg ttacataact caaccgaata
    25381 tatgaaccat cctcaatgaa atttgaactt acattgtaat aataattaat gattgtttga
    25441 ttgtcaagaa cgatgggagt cgtgtttttt acgtatgtgc catcagcctg ttctaccact
    25501 ccataccata ctacctgccg tccacgatag aattccagca ttttgtgctg tccactcgac
    25561 caaagacttc ggcccgttac attcatcaca tcccctcctt ttcgtccatc aacaagaaaa
    25621 cttaaagaaa ttcctttgta tttcagtgta ctggtcaatc ccatcaaaaa atcaggttcg
    25681 cgatttccaa tcaacacact tttggttgga ttaattttag gatagccatc gggtgtacaa
    25741 attatttctc ctttttcgtt acgcaaatag tcttttcctg aaatagctgt ggtagagccg
    25801 ttcaggtaag ccgtaggaaa aatatctccg tattgggttc cctgcagttc cacaatgtct
    25861 tcaggaaggc ttaaaacttt tccgcgatta aagccaaaat ttaaggtacc agtccacaat
    25921 aagtccttat ttttcaaaat gtcctgatcc aaagagattt caactccgtg atttttgata
    25981 ttgccttcat ttctggtttg caaaatatat cccgaagctg gcgacacacg aactgttacg
    26041 atctgattgt cgacagttgt agaataataa gctacatcta atctggtttt gctggagaaa
    26101 aagcgcgttt ccaagccaat ttcccaagag tcggaaattt caggttgtaa attgctggct
    26161 accgacatgg taggatcggc tccataacca ccatccggca aggttggata ttgagtgtat
    26221 ttacggtcga acagataggg cgttgctaca tctttaccca ctcttgccca attgccgcgt
    26281 aattttccgt aagaaaacca gtcattactt aatccgaaaa tttcggaaaa tattattcct
    26341 cctgttacgg atggataaaa gtagggattg tatttgtatg ctatggtaga agaccagtcc
    26401 cagcgcgaag taaagcttaa atttaataat cctttgtaat cacttctcag ttcgccgaaa
    26461 acaccagcat ttcttttttc ccaatggttc aacgacacat catttgttcc cacaatttcc
    26521 ctgacattgg atatactgta tgtacccggt attacaaaat ctctaccatt gatggaagaa
    26581 gaaatacgat tatcggtttt aatctctgta ccggcaagta cttctaaatt aaaatctttt
    26641 ggaagaggaa ttttgtaatt tcccatcaaa gtggcagtaa ccaattctct tttgctttgt
    26701 gaataactgt aattgccaaa caagtcctta tcaacactcg cgtagtcttt ttcattaaaa
    26761 taagggacag aataataata gtcgttgtat tctttcaaag cctgagcata agcatccaaa
    26821 ttggtgtaat cgctttcttt cggaggatta tcgagaatga tggaatcgtc aaaacgcggc
    26881 acttgatatc cgtcatacat gtaattgttt tgatcgagac ttaatcgacc cgtaatttcc
    26941 agccctttga gtggcttgta aattaatgaa ccttgtaata aattgcgggt agaagaattt
    27001 aatccgtaat ccatatacct gctccacaaa ggactaatgg gtgaattttc ttttttggga
    27061 atactgatat atcgatatcg gatgcttccg tcagaatttt tataatgggt aatatcatca
    27121 ttgattggcc aagaataaat ggcactaatg cttcctgccc ctcgataact gttctttaca
    27181 atattagtca tgaaattggc ggaaatttta ttgttgattt cgtaatttcc tttcaaaagc
    27241 aaacccattt tgtcaagata atctttcggt actataccat catgtcgtga atagtttaca
    27301 gatgcataag actgaaattt atcacttcca ccatttatac tgaaatcgta tttttgatat
    27361 aatccgcttt gaaaataatt ctttacattg ttataaacag gttcatcttt gtctaatagt
    27421 ggtccccaac caccatatcc tccactttcc tgaggtcgat agaatcccaa agcgcctggt
    27481 ccataaattg attgcaactc cagtagcctc gtaggcgtat ctgattgtat gctagcattg
    27541 gctgaaagcg acattttccc tgcttttgct gtttttgttg taatcattat tgctccatta
    27601 gctgcatttt gtccatataa tgctgaagca gccgctcctt ttaaaacggt tacattttca
    27661 atatcactgg gattaatgtc tgctgctgca gaacctccac cggaaagaac catcccatcc
    27721 agaataaata aaggctcatt gttttggctt gggttgatgg aagaaattcc tctcaataaa
    27781 atttgtgaac cggcattggg ggaacctccg gaactggtta cactaactcc tgaaattttt
    27841 ccttgcaatg aattgacaaa attaggagaa cgtccgtctg taatagcttc agcatctacc
    27901 gactgtactg caaaatttaa tttctttttc tctgctttca cacccatagc agtaacgatt
    27961 acttcatcaa tggcaattgc tgtgggattc attattacat ctattttgcc ctctccagta
    28021 actttgcgtt ctactttatc catcccaaca taactaaata ccaaagtagc tcctatagga
    28081 actgaaattg tataaacccc attataattt gtaatggtcc catttgtcgt tcctttttcc
    28141 agaactgaag cgccaatgat aggaagtcca tcttccttag atgttaccgt acctgtaact
    28201 gttaccttct gtgccaaaga cacttgaagg gaaagaaaaa gaacagcaaa taatagaaga
    28261 atttttttca taggtactaa aataattaat tgtttgaatt gcgttgtttg atgtagcctt
    28321 ttttattagg gaaataatgt ttttaataaa catttgtgat tgcaaataaa caaaaaaaat
    28381 cttttactgc aaacgtttgt tttctattta acaaaatttt aatgttaaat tttattttga
    28441 attttgtagg catctatggc ttttttcact gagccatgaa tcaacagtat atttcgggct
    28501 tgttcgtagt cgatattcag ttcttctaca atgattcttg tgccgcgatc aatcaacttt
    28561 gaattggtta gttgcatgtt gaccatttta ttgcctttca ctcttccgag ttttatcatg
    28621 gtaacggtgg aaatcatgtt gagtgtcaac ttttgggcag ttcccgattt caaacgtgta
    28681 ctgcccgtta caaattccgg cccgacaatg agttctattt ttaagtcggc ttcgtgagat
    28741 acgggcgaat cgggattgca ggcaatggaa gctgtcaaaa ttccatgttg ttttgctttt
    28801 ctcaaagctc ccatcacata gggtgccgtt ccggaagcgg tgatgccaac tacggtatcg
    28861 atggaagaaa tattgtgttt tttcaaatct tcccaacctt gttccatatc gtcttcagca
    28921 aattcaaccg cattgcgtaa agccgtatca ccacctgcaa tcacgcctat taccagatgt
    28981 ggaggcatgc caaaagtggg tggaatttca gaagcgtcca atacacctaa acgtccgctg
    29041 gtgcccgctc cgatatagaa taaacgtcca cctattttca ttcgttctac cagtttttca
    29101 atcagttgct caatttgtgg aatggctctt tctactgcaa atggtacttt tttgtcttct
    29161 tcattcattt caacgagcaa ttggcgaatg tcttttttgt ccaaatcgtt gtgcagcgaa
    29221 ttttgttcgg taattttttc aaatgccata ttttcaaata attatttgtt tttttgcgaa
    29281 tgatatttaa tcaatccctt cattggattt tgcgctatat caattattct gaaatgtctt
    29341 tcccgtgcaa tggtttgcaa aatgtcagca aagtgaaaag ccactgatcc gacaaatcct
    29401 aagggtgttt tttcactatg tgggtattgg caaatatttc tgttaaaaaa atcgttaaag
    29461 ctgctatata caatattata taaaggcgga tattgaatat tttcctttat aaaaggcaca
    29521 aattgagcca aatagcgatt cgggaaaggc tttttgtaca catggtcgag aaccttttcc
    29581 aatgtcaaat catattcttt catgaatttt gacaccaaat cgggcggtag ttgatttttt
    29641 aaagcatcag atgtcagttt tttcccaaga atggctccac tgccttcatc tcccagaata
    29701 aatcccaatg gcggaacgtt tttttctatt ccttttccgt tgtaataaca tgaatttgaa
    29761 ccggttccaa gaatacaggc aattccgctt tcctttccaa acaatgagcg agcggctccg
    29821 agtaaatcac tttctatggt tacgacaagc tcttttccca aaatatctct aaaaatttcg
    29881 cttaacatct ttttttgttc ggcaaaggcg caacctgctc cgtaaaaatg aactccatca
    29941 atcgtacttt gcatgattcg tgggtaaaga ttttctttca gctcggcaag gatttcttct
    30001 ttggtttgat aaaatggatt gatgcctttt gtgtaaaatt cctttacggt attttcacct
    30061 tctataacac accagtgggt tttggtcgat ccgctgtcag ctataatatt cattgttcgg
    30121 taagttgaat ttaattttct gcacaaatta aagcaaaaga attcaccctg ccaaagaaaa
    30181 tcaagaaaaa aacaaaattt aacgcaaacg tttgcatttt aattgaaatt gtattacttt
    30241 tgaaaccaaa ttcatcatta tcaaggtact tatgagaaca aatttaagct caaaaattac
    30301 gatgacgcgt atacctcgac gatattatca acccgatgat gtctttgaac tttcgagggt
    30361 tacccgtctt gaaaaaatac cgaccgaaat ttttgacaca gaaaaagaag gctcggcaaa
    30421 agtggcagaa gaaatagcag ccttaattaa aacaaaacag gcagcccatg agaagtgtgt
    30481 tttaggtctt acttcgggta aatcgatggt gggaatttat gctgaactgg ttcgattgca
    30541 caaaaaggaa aacttgagtt ttaataatgt tgttgttttt ttattgtttg aatactatcc
    30601 gctcaccggc aaattaaaag gaagttatgg acaattaaaa aacatgctgt tggatcatgt
    30661 tgatattccg gaagaaaata ttttttatcc ggatggcaca atggacaaac atcagattat
    30721 gcaagcttgt gctgaatatg aagaaaaaat agcttctttt ggcggtatcg atttacagct
    30781 tttgggaatt gggcgctctg gaaatgttgg tttcaacgaa ccgggctcat tggcaaattc
    30841 tcaaacccgc cttattttgc tcgatacact ttcaagagaa gatgctgctg aatttttcag
    30901 caatgtcgag atggtccctg tttctgccat tactttggga atttcaacta ttctgaaagc
    30961 caaaagaatt gttttggttg cctggggcga acataaatct acggtcgtaa aaaatgcggt
    31021 tgagggcact atgtcagaag ccattcccgc atcctatctt cagcttcatt ccaatgtaaa
    31081 aatagttatt gatcttcatg ctgccagtga attgacgcgc atcagcatgc cttggctggt
    31141 aacatcctgt gagtgggaca gcaaactgat acgtcgtgcc attgtttggc tttgcatgaa
    31201 gttgaaaaaa ccgattctga aattgactaa taaggactac aacgacaatg gattaagtga
    31261 attattggct atttatggct ctgcctacaa tgtaaatatc cgaattttta acgatttaca
    31321 gcacaccatt acaggatggc ccggtggaaa accaaatgct gatgattcta atcgtcccga
    31381 aagggcactt ccttatccca aacgtgttct catttttagc ccgcatcccg atgatgatgt
    31441 tatttcaatg ggtggaactt tcaaaaggtt aatagatcaa aatcataaag tgcatgtagc
    31501 ttatcaaaca tctggaaata tcgcagttgc cgatcatgaa gtcattcgct tcctttcttt
    31561 tgtgaaagga tttaaagatt tattcgacag caataatgat gtaattcaga ccaaatatga
    31621 agcaatgaag aattttctcc tttacgaaaa aaaggaaaat gatatggata caccagaaat
    31681 tctcgaagta aaagcattaa tcaggcgggg agaagcaaag gcagcttgta gatatttggg
    31741 tttaagctct caaaacattc attttcttaa tcttcccttt tatgaaacgg gaacagtaaa
    31801 gaaaaacgat ttatcgcaaa aggatatcga cattattaaa gacttgctgc agcagattca
    31861 accccatcag atttacgcag ccggtgattt ggccgaccct cacggaactc acaagatttg
    31921 cttggatgcc gtattggccg ctatcgacga actgaaagat gaagaatgga tgaaagactg
    31981 ccgagtttgg atgtatcgtg gtgcatggaa agaatgggaa atagatcata tcgaaatggc
    32041 tgttccctta agtccggaag agttgaaaaa caagcgaaat gctattttgc gtcatcaatc
    32101 ccaaatggaa agtgctccct atttgggaaa cgatgagaga cttttctggc aacgttcgga
    32161 agaaaggaat cgggctaccg ccgaattgta cacccagttg ggtttggcct cttatgaagc
    32221 cattgaagcc tttgtccggt ataagccagt ataattattt gtctgcgtta caaatcaact
    32281 tattaaatat ttgttatgaa acccattaat tgttttattg tttacgatga tgctgttcag
    32341 gctgaaatta ccatagaaaa tttgttgcag tcggaattgg ttaatgctgt gtatttgctt
    32401 tccaagcgag aaaatattca ggtcaacgaa aaatgttttc cgattttgat cgattcgctt
    32461 tacagcagca atacaattag gaaaattgct gaaaaatccg atacgcctta ttcgcttgtt
    32521 tatttgaaaa atactccttt acatctggga cagtttgctt tggaacgttt ttttcaaatt
    32581 gctcaggata caaaggcggg aatggtttat tccgattatt atcaagtgtc gggaggcgtt
    32641 cgcagcaaat taccgttgat cgattatcag accggaagcg ttcgcgacga ttttaatttt
    32701 ggctccgttt tgttttttaa tgcggaggtg ttgaagcagg cctctgccca aatgaacgaa
    32761 aattttcttc atgccggttt atatgatgcc agattaagaa tttcacagaa agccgaattg
    32821 gttcatatcg atgagtattt gtattcggag gtagagtcgg ataatcgaaa aagtggagaa
    32881 aagttgtttg attatgtaga tccgaaaaat cgccagcgtc aattggaaat ggagcatgcc
    32941 tgcaccgaac atttgaaaaa tatcggtgcc tatctcaagc cacagtttaa aacaattctt
    33001 ttcgatgaaa acaattttga gatggaagct tccgtaatca tcccggtaaa gaacagggca
    33061 accaccattg aagatgccgt tcgttcggct ttgactcaaa aaacgtcttt ccctttcaat
    33121 gtgattgtgg tggataatca ttctaccgat gggacttcgg aaattgtgga aaaattggca
    33181 aaagaagaca gtcgagtgcg gcatataatt cctcaacgtg aggatttggg tattggcggt
    33241 tgttggaatg ttgccgttca ttctcctttt tgcggaaaat tctccgttca gttggatagt
    33301 gacgatgttt atcaggatga aaatacgctt caaataatgg taaatgcttt ttacgagcaa
    33361 aattgtggta tggttatcgg ttcttatacc ataacagatt ttgaaatgaa tgtgataccg
    33421 ccgggcttga ttgatcacag ggaatggact cctgaaaacg gaagaaacaa tgctctgaga
    33481 attaacgggt tgggtgcacc aagagctttt tatacaccgc tactgagaaa aattaaagtc
    33541 cccgattcca attatggtga agattatgct ttaggactgt attttagccg tgaatatcaa
    33601 attggcagaa tttttcaatc ggtttatctt tgccgtcgtt ggcgcgataa ttctgatgct
    33661 gagcttgata ttgttacttt gaacacgtac aatcattata aggataaaat tagaaccata
    33721 gaaatcaaag ctcggcagca aatcaataaa ttgtcctgaa aaatgaaaat tcttaatgca
    33781 aaaaggagga gagatcaaaa atgattttcc tcctttttag tttttcgaaa tcattatttc
    33841 acaaacagaa aaaataattt cctttcgttt tttgaattgt aaacatgtag aaaaattaaa
    33901 cgattccctg agccagcatg gccctggcaa cttttatgaa tcctgcaaca tttgctcctt
    33961 tcaggtaatt aatatatccg tcttcttctt ttccaaagtt aacgcattgc tcgtgaattt
    34021 tgttcatgat ttgatgcaat tgtttgtcca cttcttctgc cgtccaagaa aggtgcatgg
    34081 cattttgtgt catttccaaa cccgaaaccg acacgccacc ggcatttaca gcttttcctg
    34141 gagcatacat tattttgttt ttgataaata aatcaatggc ttcgggtgta caacccatgt
    34201 tggaaacttc ggccacacac caaaccttat tttcaatcag attccgcgca tcttcttcgt
    34261 tcagttcgtt ttgtgtggca caaggcaggg caatgtccac ttttaattcc cacggacgtc
    34321 gatttggtat aaatggaacg ccaaactttt cggcataagg agaaacaatg tcgttacaac
    34381 ttgcccgaag ctccaacata taatcaattt tttcgccgga aataccgttt tcgtcatata
    34441 cataaccatc cgggcccgag attgaaacaa cttttgctcc catttgtgat gctttcagcg
    34501 cagctcccca tgccacattt ccaaaaccgg agatggctac ggtttttccc cttatatcca
    34561 tacctgctct ttgaagcatt tctcttacaa aataaagagc tccaaatccg gttgcttctg
    34621 gacgaattag agagccgcca aattccaaat tttttccggt taaaactccc gtattttcat
    34681 gggcaagttt gcgatacatt ccatacaaat agcctatttc acgagctcca acaccaatgt
    34741 ctcctgccgg cacatccgtt tcggggccta tatatcgcca caattcggtc atgaaagctt
    34801 gacagaaacg catcacttca gcattcgatt ttccttttgg attgaaatcg gaacctcctt
    34861 tggcacctcc cagaggtaaa gttgtcagcg catttttaaa tatttgttca aaaccaagaa
    34921 atttcattat ggaaagattc acggaagggt gaaaacgcaa accgcctttg taaggtccga
    34981 tggcattgtt gaattgcaca cgatacccaa tgttgacctg aacattcccc tgatcgtcaa
    35041 cccaagggac tttaaaagta aaaattcggt caggttcaac caacctttct gcaatttttg
    35101 ccttttcaaa ttcgggatgt tgagaataca cttcgttaat ggtggtaagc acttctctta
    35161 cggcctgtaa atattcgttt tcgttgggat gtttcgccga aagaacctgc ataaattttt
    35221 ctatgtccat aatattagat tttagttttt tgttgaaata tagatttgtg tagctcaaat
    35281 ttttgaaaat ttttacatat tgtcatttat ttcttggttg acaagcataa agttgttggt
    35341 ttttcttgaa aattttgatt tcatttgtgc tttttctgtc attttatttc tttgcaagtt
    35401 taaaattttt atttgggatt atcaaacttt ttgagctaat tttgaaaaaa tatttcgata
    35461 aacttgtgtt ttgaagttca gttgattttt ttattgagat tcattggctt caaaataaca
    35521 gaaaacattt atttatgaac actttaagta ttaataagtt atattttaaa gacacttctt
    35581 ttgccaactt aatgacgcat cggatttata atattttact ttatgccagt aaatacgatg
    35641 cttttgtgct ggaagaagac ggacgcattg atgaacaaat tttcaacgaa tatacagctt
    35701 tgaatttgcg ttatccgccc cgttttacac ttgtttcgaa tgaggaggag gccaatgagt
    35761 tgcttcgtac ccgtactttt gaactgatta tttccatgcc gagtggcgat agtataaatc
    35821 cttttgaatg ggcaaaagat gtaaaaaaag aatatcccga aatccctatt gtggtgctta
    35881 ctcctttttc ttcatccatt tctcaacgat tggcacacga ggatttgagt gccatcgatt
    35941 atgttttcag ctggttgggt aattccgata ttttgttggc tatcatcaaa ctgattgaag
    36001 acagaatgaa tgtggaacaa gatgtcaatt cggttggtgt tcaggttatt ctttttgttg
    36061 aggactccat acgctggtat tcatccattg ttcctcattt atacaaatac gtttttaagc
    36121 agtcgcgttc atttatgact gaggctttga acgaacatga gcaaatgtta cgcatgaggg
    36181 gacgcccaaa aattttgttg gcacgctctt atgaagaagc tatttccatt tataacaaat
    36241 atagccagaa tatgcttggc gtgattacgg atgtaagttt tccggaaaaa aatgtcaaag
    36301 acaaaaaagc cggtctacat ttatgcagcg aaatacggag aaatgacaaa tttattccgc
    36361 tgattgtggt ttctactgaa gaagaaaatc gacaaaccgc agaagaattg aatgctgttt
    36421 ttctgaacaa aatgtcgaaa acgcttttta tggagttgcg ggaaaaaatc acagataatt
    36481 tttggtttgg cgatctgata ttttttaatc ctcagacagg ggtggaagaa gcacgcgttc
    36541 acaaccttag aagtttacag gactgtattt ttaaggtgag cgacgaagct ttgtattacc
    36601 atgtttcccg taacaacatc tccaaatggc tgtattcgag agccatgttc ccattggcag
    36661 aatttctgaa aaatattcaa gttgaacccg gaatgtccga aaacatgaaa aatgtacgtc
    36721 aaattatttt tgatgccatt gttcattacc gaaaaataaa aaatcgtgga atcgtagctg
    36781 tttttcaacg cgacaggttc gacatgtatt ctaattttgc ccgcattggc gaaggttcat
    36841 tgggaggcaa ggggagaggg ttggcttttt tggatgccat gatcaaacgc aacgaaagtc
    36901 tcgataagtt cgaaaatgtt cagattgcca ttcccagaac ggtcgtactg tgcaccgata
    36961 ttttttccga cttcatggaa atgaatcagt tatatcccat tgctttgtcc gatattcccg
    37021 acgaagaaat tctggcacat tttttaaaag ctcgattgcc acagaaactt ttagccgatc
    37081 tttatgcttt tttgggagct gtaagcaaac ccattgccgt aagatcctca agtttgttgg
    37141 aagattctca ttatcagcct tttgcaggag tttattcaac ctacatggta ccttttgatg
    37201 ccaacagcaa aactcataca ctgcaaatga taacggaagc cattaaagct gtttatgcct
    37261 cggtttttta tcgtgacagt aagacttaca tggcagccac caagaatttg atcgatgaag
    37321 aaaaaatggc cattgtgttg caggaaatat gtggcagtgc ttacgaaaat cgttattatc
    37381 cttctttttc gggagtagct cgctcgttga actattatcc gattggaaat gaaaagcctg
    37441 aagatggaat tgccaata
//
LOCUS       DAOSIB010000011        37283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_1273_length_37283_cov_11.395858, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000011 DAOSIB010000000
VERSION     DAOSIB010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 37283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37283
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1273_length_37283_cov_11.395858"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(389..1639)
                     /locus_tag="PLD44_01960"
     CDS             complement(389..1639)
                     /locus_tag="PLD44_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98136.1"
                     /translation="MNINQQIIDNLHSPEELEKIYREHKREFSDFFKNNKGNYDSELY
                     RFWQVRLAAETSHSRHKALKSDIIFLMLVSLFSGLLLKLPDFFAQLDKNSFYLRNLSL
                     ILFNALILYAFWQNKLFKRHEMLIYSISIVALAIYVNLLPVDKSDAVRLALLHVPVLL
                     WYLFGWVLVSFDRNNLNKRIGYIRYSGEYLVMTGLILIGGALLTALTINMFSAIGMNI
                     ENFWMEYVVIFGGAAMPVVSAFLIHIYPDVTGKITPVLARIFSPLALVTLAVYLVFLL
                     MGNSNIMKDRNLLMALNVVLFTVLVLVVFSISEIDQSKKKNAGVFVLFLLTLLAILVN
                     VVALIAIVSRLFNGFTPNRTVVLGFNVLIFINLILLLPKLFRSFSHPEMLEEVEQSVA
                     RYLNVYFLWTLMVVFVLPVLFRFR"
     gene            2037..2945
                     /locus_tag="PLD44_01965"
     CDS             2037..2945
                     /locus_tag="PLD44_01965"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOF98137.1"
                     /translation="MNKQTHKRTPLPSTKKGQEMKVSENDLLLDFLLKSMNHKSRNSV
                     KSLLKHGQIVVNGKTITRYDYPLKLGDIITVGNHQSIVEKNIPQMRILYEDDDIIVID
                     KEAGLLTITTGNGIDITAVSLLKDYVQKQSPYNKIYTVHRLDQMTSGVLVFAKNSEAQ
                     HQLRDNWHNMVTKRTYVAVVEGKMDESAGKISSWLTENKKNYMVYSSPVDNGGKLAIT
                     RYKVLQTSDQFSLLELELETGRKNQIRVHLKDLGHPIVGDRKYGASTSIGRIALHARV
                     LEFYHPVSGEIMHFETPVPHLFLRLL"
     gene            complement(2965..3603)
                     /locus_tag="PLD44_01970"
     CDS             complement(2965..3603)
                     /locus_tag="PLD44_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOF98138.1"
                     /translation="MKILKNIVFDFGGVLIDWNPRYLYRNVFETEDEMEYFLEHICPY
                     DWNLMQDAGRLLAEATLSKQKEHPEYSNEIALYYGRWIEMIGGIFEENVKLLKPLKEK
                     YNLYGLTNWSAETLPLAQAKYDFFDLFDGIVVSGAEKIVKPDPELYRILIDRYRINPQ
                     ETLFIDDNPQNIQTAERLGFHTLYLTPQINLEQELKNRGIIDCWKKEFRSEK"
     gene            complement(3779..4192)
                     /locus_tag="PLD44_01975"
     CDS             complement(3779..4192)
                     /locus_tag="PLD44_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOF98139.1"
                     /translation="MKSEVRIDKWLWAVRLFKTRSLAAEACKKGKILIHGQPVKASHS
                     IRVGDVVGVKRNPVLFSYQVLALTENRLNAKLLPEYLLDVTTPDQRELFELGKLNNSG
                     RIRGSGRPTKKERRDLEDFVEPFFLDDDEDGWEDL"
     gene            complement(4205..4768)
                     /gene="pth"
                     /locus_tag="PLD44_01980"
     CDS             complement(4205..4768)
                     /gene="pth"
                     /locus_tag="PLD44_01980"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOF98140.1"
                     /translation="MKFLIVGLGNIGSEYENTRHNIGFRILDAFAEASNVFFTDQRYG
                     AVSEVKLKGHTLILLKPSTFVNLSGNAVRYWMQKEKIDVEHLLVVVDDIALPFGTIRM
                     KPKGSDAGHNGLKNIQEILGHNHYARLRFGIGNDFPRGRQVEYVLGKWTEEEAAQLPE
                     RIEKAIEVIKSFCLIGVEKTMALYNNK"
     gene            complement(4889..5461)
                     /locus_tag="PLD44_01985"
     CDS             complement(4889..5461)
                     /locus_tag="PLD44_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOF98141.1"
                     /translation="MKEFDLKGALRNGFGKKASKLERSSEKVPCVLYGNGENVHFTVS
                     EVDVLKLIVNPDVFVVNLDIEGKKCKAIVKEVQFHPVTDKILHIDFFMISEAKPVVVE
                     LPVKLEGLAEGVKAGGRLSLEMRKLKVKGIYTKLPEKVVIDVSSLGLGKTIQVGSLSF
                     NDFEILNAKNAVVAQVKVTRASYAAAAGGK"
     gene            5850..6377
                     /gene="infC"
                     /locus_tag="PLD44_01990"
                     /pseudo
     CDS             5850..6377
                     /gene="infC"
                     /locus_tag="PLD44_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
     gene            6435..6632
                     /gene="rpmI"
                     /locus_tag="PLD44_01995"
     CDS             6435..6632
                     /gene="rpmI"
                     /locus_tag="PLD44_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOF98142.1"
                     /translation="MPKKKTNSGAKKRFTLTGTGKIKRKHAFKSHILTKKTKKQKRNL
                     THSALVHKANLESVKELLNLK"
     gene            6719..7069
                     /gene="rplT"
                     /locus_tag="PLD44_02000"
     CDS             6719..7069
                     /gene="rplT"
                     /locus_tag="PLD44_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOF98143.1"
                     /translation="MPRSVNHVASRKRRKRILNLTKGYFGSRKNVWTVAKNTWEKGLL
                     YAYRDRKAKKRSFRALWIQRINAAARLEGSSYSRLMGALHKSGIVINRKVLADLAMNH
                     PEAFKAIVQKAKNA"
     gene            complement(7306..8304)
                     /locus_tag="PLD44_02005"
     CDS             complement(7306..8304)
                     /locus_tag="PLD44_02005"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOF98144.1"
                     /translation="MKTKVILFDAKSYDEIFFKEKNQQYGFDIKFQKSHLSKDNAILT
                     KGADVVCIFVNAEVDAEVIDILVENGVKLIALRSAGYNNVDLKAAKGKIKVVRVPAYS
                     PHSIAEHTIALMLTLNRKIHKAYTRTREGNFALEGLMGFEMNGKTAGIIGTGKIGKIV
                     IQILKSMGMNVLAYDLYPDTQFAETNGIRYTQLDELYGLSDVISLHCPLTKETEYLIN
                     EKSIHKMKEGVMIINTGRGKLIKTKALIDALKSKKIGYAGLDVYEEEGDYFYEDHSDI
                     VIDDDILARLLTFNNVIVTSHQAFFTKEALSEIARITLQNIQDFVDGKPLVNEISA"
     gene            complement(8435..10483)
                     /locus_tag="PLD44_02010"
     CDS             complement(8435..10483)
                     /locus_tag="PLD44_02010"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOF98145.1"
                     /translation="MKLKVNKKKFETVLKLLLAFALVIIIVELYPHESTFNYQFEEGK
                     PWSYELLTAPFDFPIYKSNEQIEKEKKEILKNFTPYFNLDTTVMHIQVNKFKIDSSRS
                     GNISPDLTTFIEEKLKSIYSKGIISNDDYNNLQKWNVKKIYRILPGRISQLATLNEIY
                     TPKTAYEEIVKSNQENIKELDINNYLVENLRYDSITSETAKNELLKNLSLTVGMVQSG
                     ERIIDKGEIVSPEAYAILRSLQIENENRKSAFEQTAIVLTGKILLVIGLITLLILYIY
                     LFRPRILDNFNNLLFLSLLIILIVGLTSLTIRYSSLSYYIVPFALLPIIVRVFYDSRT
                     ALFVHIIVIFIVSFMTDNPFQFALLQITAGMVAVSSLKDMTQRSQLAQTALYIFLSYT
                     VVYLSLELMTEGNISKIDWYPIISFAVSSLFLLFAYLLIYIFEKMFGLLSAVTLVELT
                     NVNSDLLMKFAEVAPGTFQHSLQVSNLATEAAKKINANSLLVRTGALYHDIGKMKNPE
                     FFTENQIGGKNPLLSMQYEEAAKLIIEHVANGVEIARKNHLPNQIINFITTHHGTSKT
                     KYFYVSFINDNPGIEPPEDVFSYPGPLPNTKETAILMMADAVEARSRTLSEYTEESID
                     QMVESIINTQIAEGQLKDAPISFRDVETVKKVLKEKIKSIFHSRITYPEINKNLSTNV
                     "
     gene            complement(11334..12881)
                     /gene="dnaB"
                     /locus_tag="PLD44_02015"
     CDS             complement(11334..12881)
                     /gene="dnaB"
                     /locus_tag="PLD44_02015"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOF98146.1"
                     /translation="MAEKKTYNKKQNSAPTVIAPNEMGKLPPQALELEEAVLGAIMIE
                     KDAYFLVSEILKPVCFYKPTHQKIYEAIMTLALHQEPIDMHTVTEQLRRDGTLEEIGG
                     PYYITLLTAKVSSAAHLEYHAHIILQKFLARELIRISSEIQTKAFDDKTDVEDLIEES
                     EEMLFEIAQRNLEKEVIQIKPVIDEAIQRIQAAANKQGTSGVPSGFHSLDKITSGWQK
                     SDLIIIAARPAMGKTAFVLSMAKNMAVDYNVPVGVFSLEMSSVQLANRLIMNVCEIDG
                     VKIKNGDLSPYEWDKLDKNIKDLIDAPVFIDDTPSLSISELRSKARRLVKEHKVQCIF
                     IDYLQLMNASEMRFSSREQEVSTISRSLKSLAKELDIPIIALSQLNRSVEGRSGVDGK
                     RPQLSDLRESGAIEQDADVVCFIHRPEYYNLTTDKDGNSMIGIAEIIVAKHRNGATDT
                     IHLKFKKEFAKFLNLDDIDSDDIFNENYQVISSKMNVPEGNFYENTSGSPALDNLLKN
                     NNSEVPF"
     gene            13160..14893
                     /gene="lysS"
                     /locus_tag="PLD44_02020"
     CDS             13160..14893
                     /gene="lysS"
                     /locus_tag="PLD44_02020"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOF98147.1"
                     /translation="MNIELSDQEQYRRQSLRELRNLGIEPYPAALYPTNAFSTDIKSN
                     FDENNPQRMEVCVAGRIMSRRIMGKASFVELQDAKGRIQIYITRDDINTEAQPEMYNT
                     VFKKLLDIGDFIGIKGYVFRTQTGEISVHAKELTVLSKSLRPLPIVKYKDGVAYDAFD
                     DPEQRYRQRYVDLVVNEGVKDIFLKRTKVYQSMREFFNTRGYVEVETPILQPIPGGAA
                     ARPFITHHNALDMPLYLRIADELYLKRLIVGGFEGVYEFSKNFRNEGMDRTHNPEFTA
                     MEIYVAYKDYFWMMDFTEQMIEKICLDVNGTTEVQVGDKTISLKTPYKRISMIDAIKE
                     NTGIDIEGMDENDLRKVCEDLQIDVDEMMGKGKLIDKLFGEKCEANYVQPTFITDYPL
                     EMSPLCKRHRNNPELTERFELLVNGKELANAYSELNDPIDQLERFQEQLRLSEKGDDE
                     AMFIDMDFVRALEYGMPPTSGMGIGMDRLVMLLTGQQAIQEVLLFPLMKPEKVQKVDT
                     KEKFMELDIPEAWAEVLQKMGIKQVDQLKDLNPNKLHQDICGFNKKNKLALTNPSKEE
                     VSQWIDNAAKK"
     gene            15273..16238
                     /locus_tag="PLD44_02025"
     CDS             15273..16238
                     /locus_tag="PLD44_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOF98148.1"
                     /translation="MFEIVFELSSLADTAVFYGINNSNMQLIKNLHPNLRIVARGNAI
                     KIIGNQQDADKLIVNLQKLEKFSIDNNQLTEKNIIELIKGENPKDISFQNLIMHGVGG
                     KSIFARTKNQQRLVEEFEKNDLLFAIGPAGSGKTYTAIALAVRALKNKQIKKIILSRP
                     AVEAGEKLGFLPGDMKEKIDPYLQPLYDALQDMITPLKLKEYMENGTIQIAPLAFMRG
                     RTLSDAVVILDEAQNSTIMQIKMFLTRMGLNTKMIVTGDITQIDLPRDQKSGLIDALN
                     LLGNIKGIARIDFDVNDIVRHRLVQQIVQAYDNRAMNERQKNTKE"
     gene            16312..17265
                     /locus_tag="PLD44_02030"
     CDS             16312..17265
                     /locus_tag="PLD44_02030"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOF98149.1"
                     /translation="MNTLVRTDFHFEGQTNVYHGKVRDVYFIQDDLLVMVATDRISAF
                     DVILPKGIPYKGQILNQIAAKFLDLTADIVSNWKLACPDPMVTVGLKCEGFPIEMIVR
                     GYLCGSAWRAYKNGVREICGVKLPENMRENQRFETPIITPTTKAELGTHDEDISRQEI
                     LAKGLLTPEEYDLLEKYALALFERGTQVAAERGLILVDTKYEFGKRNGNIYLIDEIHT
                     PDSSRYFYAEGYEERFEKGEPQKQLSKEFVREWLMENGFQGKEGQKVPEMTDEIVNSI
                     SERYIELYEQITGEPFQKTDTRNLAERIESNVVKYLQSLKK"
     gene            complement(17359..18912)
                     /locus_tag="PLD44_02035"
     CDS             complement(17359..18912)
                     /locus_tag="PLD44_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOF98150.1"
                     /translation="MFKNHPKGLIAASLANMGERFGFYTMMAILSLFLMAKYGLDETK
                     AGLIYSVFYFSIYILAFVGGLIADKTRNYKRTILWGLILMSIGYFLVALPTPTPVPQN
                     RYGFFLGLTCFGLLTIALGNGFFKGNLQALVGQMYDNPKYEKMRDSGFSLFYMFINVG
                     AIFAPFAAIGVRNWWLHSHGFAYNPDLPALCHDQLNGIITPEAAQRMHELAVNAGYTG
                     SNMTSFADQYLNLFTTGFHYAFMVAIGAMFVSLIIFVTNKKSFPQPRNLSSTATVTLD
                     QNGKEMDAKEIKQRLYALLAVFAVVIFFWFSFHQNGLTLTFFAKDYTNLSKVSVNLGF
                     TTLQGAEIFQSINPFFVVFLTPLVMALFGYLRSKGKEPSTPRKIAIGMGIAALAYVVM
                     TLGSLGLPSKEEITLRGGLPDAERVTPLLLLGTYFILTVAELFISPLGISFVSKVAPQ
                     KYQGIMQGGWLAATALGNQLLFLGALMYKNIPIWMTWSVFVGACLISMFTMLAMLKWL
                     ERITGEKQG"
     gene            complement(19060..19590)
                     /locus_tag="PLD44_02040"
     CDS             complement(19060..19590)
                     /locus_tag="PLD44_02040"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOF98151.1"
                     /translation="MQSNKDNRIFLIGYMGAGKTTIGEQLAERLGYKFLDMDHVIEEK
                     MQQSVSQIFSEKGEKEFRRLERECLDKISTEQNVVVATGGGAPCFYDNMERMNAYGLT
                     VYLCLSPEQLQERLELSHKNKRPLLAQREGKELLDFIRQNLSEREPFYRKAALVVSGT
                     DEEMFQQILSFLSSHS"
     gene            complement(19771..20313)
                     /locus_tag="PLD44_02045"
     CDS             complement(19771..20313)
                     /locus_tag="PLD44_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98152.1"
                     /translation="MKKQFVLLSFMAVVLLTGCNIDGNSNYTPAIQFLAFPYTQHGDT
                     LNIKTTNESGVYLLDSIQVGDTVFFQLGLNAYTNNLVSFRLTKTVDSAGDFLFPPVAS
                     LDSVFSHTNSDYGQGKFVFLSGFNAVYFPFHFAAKSATNDEILQFSLVSDAQFDDVYG
                     SNVTTFKLKTPIKPAAVVEE"
     gene            complement(20393..20465)
                     /locus_tag="PLD44_02050"
     tRNA            complement(20393..20465)
                     /locus_tag="PLD44_02050"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20430..20432),aa:Pro,seq:cgg)
     gene            20668..21396
                     /locus_tag="PLD44_02055"
     CDS             20668..21396
                     /locus_tag="PLD44_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOF98153.1"
                     /translation="MFTEAIQNYLQRHDYKSFYPKAVFFDMDGVLIDSMKNHSRAWMQ
                     AMKESNLPFTEEDAYMHEGCIGATTINDTFMKHLNREATEEEQKQIYSAKSRIFESLE
                     PIEKMPFAYELLKKLKSEEMKIYVVTGSGQPKLIDSLNTLFPEIFTKETMITGFDVKE
                     GKPSPEPYLLALKRSGFQPWETIVVENAPMGILSASKAGLFTLVVNTGPFDDDFLYSY
                     GADLVLDSMEHLYNEWDNFRKSWI"
     gene            21398..22429
                     /locus_tag="PLD44_02060"
     CDS             21398..22429
                     /locus_tag="PLD44_02060"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOF98154.1"
                     /translation="MKKTGLELFLSPVFLSLILFFSSTVIFAGETDSASFHPQIEGTI
                     RAKYEYNTSLNASRFQVRNARFSVNGNFNSLVSYKTEIDLSDEGQTKMLDAYIRLQPQ
                     KWFTFTIGQQKIPFSTDNLRPPHLLYFANRSFIGKQLTALRDVGTTLSFVNNKTLPFE
                     FITGLYNGEGLYNQKIWQTSFSYAFRLVLHPCENLHFSLNHNSIRPEEMRMNLYDLGG
                     ILQLKNWHLETELFYKTYQQNFFPTTKGFFVFSSYDIYLHNKESIKIIRPLIRYDSMT
                     DNNKGYKSENGSYESDDMARSRITGGITLCLDKPFLNDIRLNYEQYFYKKGIQNNDNK
                     FVVEFVLRF"
     gene            22502..23815
                     /gene="hisD"
                     /locus_tag="PLD44_02065"
     CDS             22502..23815
                     /gene="hisD"
                     /locus_tag="PLD44_02065"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOF98155.1"
                     /translation="MQVIKYPAREFWSTLLSRPAIDRKSLFEKVGSIVSEVKQNGDKA
                     VKKYTLQFDKVGIEELEVSQEEIEEASLQIPNQLKEAIEMCRRNIWKFHSAQQHDLPE
                     IQTTPGVVCWQKAMPIEKVGLYVPGGTAPLFSTVLMLGVPAQIAECKEIVLCTPPDKE
                     GKIHPAVLYAAKVAGIHRIFKIGGIQAIAAMAYGTETVPKVYKIFGPGNQYVTAAKQL
                     IALDGIAIDLPAGPSEVMVIADESARPCFLAADLLSQAEHSVDTQSILITVSENLVEP
                     VLLEIEEQLNRIPKKEIALKALENGKIIVLQSLEEAVAMANEYAPEHLIISTRYSLGV
                     ASNISNAGSVFLGHYTPESAGDYASGTNHTLPTNGYAKAYSGVNLDSFMRKVTFQQIS
                     REGLSNLSNAIILMAENEGLYAHSNAVKVRLDKEESEEKPENRLC"
     gene            23891..24925
                     /gene="hisC"
                     /locus_tag="PLD44_02070"
     CDS             23891..24925
                     /gene="hisC"
                     /locus_tag="PLD44_02070"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOF98156.1"
                     /translation="MKINELVRENIRNMQPYSCARSEFKGEASVYLDANENPYNSPFN
                     RYPDPLQTELKKEISKIKKVPVENIFLGNGSDEAIDLLYRAFCEPKIHNVVAIEPSYG
                     MYRVCAEINNVEYRPVLLDENFDVEAYKLMDASNDKTKIIWLCSPNNPTGNSLNFSEI
                     NKLLSWFRGIVVIDEAYIDFSTKKSLLNYLTLFPQLIVLQTMSKAWGNAGIRLGMAFA
                     DPEIIGILNKIKYPYNINYLTQQHALQALQNPMQVEKWVALILEEKEKLIKELQKLKL
                     VEKIYPSDANFILVKVENANAIYRFLIQNGIVVRNRSNMPLCNNCIRITVGTPEENKA
                     LIDTLKKFHV"
     gene            complement(24985..25058)
                     /locus_tag="PLD44_02075"
     tRNA            complement(24985..25058)
                     /locus_tag="PLD44_02075"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25022..25024),aa:Met,seq:cat)
     gene            complement(25392..26402)
                     /locus_tag="PLD44_02080"
     CDS             complement(25392..26402)
                     /locus_tag="PLD44_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOF98157.1"
                     /translation="MEETKYMPADFKTDQQPRWCPGCGDYAIFNALVKTMAELGVSPH
                     ETVIISGIGCSSRLPYYTNTYGFHTIHGRGACVATGVKVANPALTVWQVTGDGDCLAI
                     GGNHFIHSVRRNVDINVLLFNNQIYGLTKGQYSPTTKKGFVTKSSPYGTVERPFRTAE
                     LTFGAGGTFFGRLLDVDLKTSQMVMKAAALHKGTSVVECLVNCVIFNDGAHGWLAEKE
                     TRDDRIVVLEHGKPMIFGKNKDKGLILEKSELKVVTIGENGITDKDILVHDAKCKDTF
                     LHTKLALMEGPEMPIAMGIIRDVEEETYEMAMENQINEIKKQTSVKSFDELIDACEQW
                     EI"
     gene            complement(26436..28280)
                     /locus_tag="PLD44_02085"
     CDS             complement(26436..28280)
                     /locus_tag="PLD44_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOF98158.1"
                     /translation="MNKAQTIQLDNIVIRFSGDSGDGMQLTGNLFAQLSAELGNEIST
                     FTDFPSEIRAPQGTLGGVSGFQVHVGANKIYTPGDEIDVLVTLNPAALKVNAKNLKKG
                     SIVIFDLDSFQPSDLKKANFSTDNPFEELSLSDTIQLIPVPLTTLTKSSLEDFGFDNK
                     TVLRSKNMLALGLVCWLFNRPLDKAIHFLENKFRKKTQILQANIKALTDGYNYGENLH
                     LTVSNFVIEKSHEMPKGKYTSINGNKATAWGLIAAAEKAGLQLFLGSYPITPATDIMQ
                     ELALQKGIGVKTVQAEDEIAGITMAIGASFAGCLAATSTSGPGMSLKTEGIGLAVMAE
                     LPLVIVDVMRSGPSTGMPTKTEQGDLLQALYGRSGESPLAVLAAPTPDSCFHYAFMAS
                     KIALENMTPVILLTDAFIGNGSSLWRLPKLADLPEIHPHFVPSNMENYKVTTRNEDTK
                     IRYWSIPGMKGFQHCNGGLEKDYTTGNLSTDPENHEKMVLTRKQKVDNIVNVIPELEI
                     EGNTDADLLVVGWGGTYGHLKSAVDNLNNKGYKIALAHFTYLFPLPANTENVIGKYEK
                     IVVCELNTGQFASYLRSKIPGKTFTQFNKIQGQPFKTEELENHFISLL"
     gene            complement(28341..29747)
                     /gene="asnS"
                     /locus_tag="PLD44_02090"
     CDS             complement(28341..29747)
                     /gene="asnS"
                     /locus_tag="PLD44_02090"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOF98159.1"
                     /translation="MELMKRTKIVDALKKIDFGEDICVKGWVRTRRGNKNVSFIELND
                     GSTVHNIQIVADNNKLGIDFLKPITTGACIGVTGKLAQSLGKGQSVEIQAEDIVIYGT
                     ADPETYPLQKKEHTMEFLREIAHLRPRTNTFGAVFRIRHHMAMAIHTFFHERGFFYFH
                     TPIITGSDCEGAGQLFQVTTLNLSDLPKAENGCIDYSNDFFGKQTGLTVSGQLEGELG
                     ALALGAIYTFGPTFRAENSNTPRHLSEFWMIEPEVAFNELEENMQLAEDFVKYCVRWV
                     LEKCSDDLEFLNNRYDPELIDRLKFVIENDFVRLTYSEGIKILEDAMAKGQKFEFPVG
                     WGIDLQSEHERYLVEKHFKKPVILTDYPKNIKAFYMKLNDDGKTVRAMDVLFPKIGEI
                     IGGSQREDDYEKLKARAKEMEVPDKDIWWYLETRQFGNAPHSGFGLGFERFLLFVTGM
                     SNIRDVIPFPRTPKNADF"
     gene            complement(29937..31451)
                     /locus_tag="PLD44_02095"
     CDS             complement(29937..31451)
                     /locus_tag="PLD44_02095"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOF98160.1"
                     /translation="MRVKVLIKVSTFCLALLFLGCAGQSNKEGYSRVTGWKYNDEKGA
                     GFAVRKDFKTNVPPGMVEIEGGSFTIGEKGEVVTAPRNSRRRRITVSSFYMDQYEIRN
                     VDWREYTHWMNLVFGKTAPKLVEKTYPNGKVWREELAYNEPYIEHYFTHPAFDQYPIV
                     GVTWEQAMDYCSWRTDRVNELALVKAGIIAPPDFASLKNETNFDNIINNFVFNTQKYL
                     KQASYQPPVGKNPKKDLTGEIRKVDMSDGILLVSDFRLPTEAEWEYAAYGLKAGKDGF
                     IPEGKIYPWNGHQVRNPSRKQLGQMMANFVRGRGDMMGTAGSLNDRATITAPVNSYYP
                     NDFGLYNMAGNVNEWVLDVYRATSFEDVAEYNSFRGNMYLTPIVEKEDEVGNKIFKID
                     SLGRIATTVLEGDDVRNYKDGDLTSKISTDFQLYADSSDIAAITNMKKIDPTDVLAPQ
                     ISDKTRVYKGGSWKDRIYWLNPSTRRYLDQDQCSNDIGFRCAMSKMGNIEPVKR"
     gene            31812..32663
                     /gene="folP"
                     /locus_tag="PLD44_02100"
     CDS             31812..32663
                     /gene="folP"
                     /locus_tag="PLD44_02100"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOF98161.1"
                     /translation="MFLNLNGRLVDVSSPVVMGIVNVTPDSFYTGSRFLSEKSILTSV
                     DNILSNGGTIVDVGAYSSRPSAESVSVEEELKRLSRALEIILKQFPNVILSVDTFRSK
                     VARYVIENFHIAMINDIGGGTLDELMFETVADLRVAYVLMHMRGTPQTMQTLTHYENN
                     VVSEVFSFLQKGLAQLHLLGVKDVIVDPGFGFAKTLEQNYELLHKLHYLKELNAPILV
                     GLSRKSMIYNLLRLSPEEALTGTIAANMLSLIGGASILRVHDVKEALQTIRIFEQYQN
                     FSTFIND"
     gene            32675..33487
                     /gene="cdaA"
                     /locus_tag="PLD44_02105"
     CDS             32675..33487
                     /gene="cdaA"
                     /locus_tag="PLD44_02105"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOF98162.1"
                     /translation="MFFQIEFKDIVDIFLVAFLLYEIYKLLRNSGAINIFIGILTFIV
                     CWFFVSYVFKMQLLGEIMDTVVNVGAFALIVLFQDEIRRFFTQIGTKRENRFLSKMKK
                     IFGESEYDKKETDFDLLQVVLACRNMSRTNTGCLIVITRRNALEFYVQSGELIEAKIN
                     SRLIESIFFKNSPLHDGALIISRHTIKAAGCILPVSQNQSIPKRLGLRHRAALGITEH
                     TDAVAIVVSEETGHISWAVNGNLTHNVKPEELEYFLNTQLQGSNKVEEEKIK"
     gene            complement(33556..34548)
                     /locus_tag="PLD44_02110"
     CDS             complement(33556..34548)
                     /locus_tag="PLD44_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOF98163.1"
                     /translation="MVHYTELGLVNSRELFKKAIEGKYAIPAFNFNNMEQLQAIISAC
                     VETKSPVILQVSSGARKYANQTLLRYMAQGAVEYAKELGYNIPIVLHLDHGDTFELCK
                     SCIESGFSSVMIDGSHLPYEENIALTKKVVEYAHQFDVTVEGELGVLAGIEDDVVAEH
                     HTYTDPDQVIDFVTRTNVDSLAISIGTSHGATKFKPEQCTRDENGILIPPPLRFDILE
                     EIEKRIPGFPIVLHGASSVPHEYVEKINKYGGALKDAIGVPEDQLRRAATSAVCKINI
                     DSDGRLAMTAAVREIFATKPAEFDPRKYLGPARDELKKLYMHKTVNVLGSAGKA"
     gene            34784..35203
                     /gene="mscL"
                     /locus_tag="PLD44_02115"
     CDS             34784..35203
                     /gene="mscL"
                     /locus_tag="PLD44_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOF98164.1"
                     /translation="MKLWKEFKEFAMRGSVIDLAIGVIIGSAFGKIVSSLVADVIMPP
                     IGLLISGINFTDLKLVLKPAEMVNDVQTPAVTLNYGNFLQVAFDFLIVAFVIFLLIKG
                     INNFLKKKEEKPVPEATAQPSKEEILLTEIRDILKKK"
     gene            complement(35229..35549)
                     /locus_tag="PLD44_02120"
     CDS             complement(35229..35549)
                     /locus_tag="PLD44_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98165.1"
                     /translation="MQTINQLFQLLLKDIQEKMNDEFDRNFERNTFFAEAWNPNKHNS
                     IGLPMMRTGALRKSLRSEKIDIYSIWLQSLLPYAELHNSGKTFNRTKRKLCFCLCSEV
                     CYAT"
     gene            36399..>36795
                     /locus_tag="PLD44_02125"
     CDS             36399..>36795
                     /locus_tag="PLD44_02125"
                     /inference="COORDINATES: protein motif:HMM:NF023583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HOF98166.1"
                     /translation="MTHQNGLDVKTLENWLWEAACKIRGPIDAPKYKDYILPLIFLKR
                     LSDVFEDEINRLSQNYGSREIVEQFLSNDHNLVRFYLPPETRWEAIAKHTTGVGEYLT
                     NAVRKIARENPKLQSVIDIVDFNATAGGQR"
     assembly_gap    36796..36819
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <36820..>37283
                     /locus_tag="PLD44_02130"
     CDS             <36820..>37283
                     /locus_tag="PLD44_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOF98167.1"
                     /translation="LINVLGKYRLGLDDVEPDILGRAYEYLLRKFAEGSGQSAGEFYT
                     PSEVAVLMSYILDPKPGDEIYDPCCGSGGLLIKAFLRSKEKFDDNPSFEPVRFYGQEI
                     LHATYAMAKMNAFIHDMEAEIALGDTMNRPAFLTSAGALRKFDLVTANPMWN"
BASE COUNT        11927 a         7146 c         6782 g        11404 t
ORIGIN      
        1 caattcggtc tcccagcatg acgcacaacg tcttatttac gccataaata tacaaatttc
       61 gtaaatgcaa aatttaaaac atgcgtaaac cacttttaag tttttgctta ttattaatgg
      121 taaaaagcat gatatcaata ggataggtta ttcaaaaaaa ttacatttac gaataggtat
      181 tgaccttcct taattgtgag gttcgtatgt catttcatat ttgttttctg aatcagctat
      241 ctcagcaaaa tcttattcct tctttttctt tctctacctg cctactattt ttttaataat
      301 ttcacaaata tacacaacaa ttctattcac acaaaacaat gatttaaaaa aaattaattt
      361 tgaaatttcg actttgatat tgtccaactc accgaaacct gaacaacacc ggcaatacaa
      421 ataccaccat cagtgtccag agaaaataca cgttcaaata tcgggcaacc gactgttcca
      481 cttcttcgag catttcagga tgagaaaagg aacgaaacag ttttggcaac aaaagaatca
      541 aattaatgaa aatcaatacg ttgaaaccta aaacgacggt tctgtttggt gtgaatccgt
      601 tgaaaagtcg ggagacaatg gcgattaaag ctaccacatt gaccaaaatt gccaaaagag
      661 tcagcagaaa caacacaaaa acgcctgcat ttttcttttt cgattgatcg atttccgaaa
      721 tcgaaaaaac aaccaacacc aaaacggtaa aaagaacgac attcagtgcc atcaataaat
      781 ttctgtcttt cataatatta ctgttcccca tcaaaagaaa aaccaaataa acagccagcg
      841 taaccaatgc gagcggtgaa aaaatgcggg caagaaccgg tgtaattttc ccggtaacat
      901 ccggataaat atgtatcaga aaagccgaaa caacaggcat ggcagctccg ccaaaaatta
      961 caacatattc catccaaaaa ttttcgatat tcattccaat agcagaaaac atgttgatag
     1021 tcaaagcggt gagcagtgcc cctcctatca ggattaatcc tgtcatcacc aaatattctc
     1081 cactgtaacg aatataaccg attcttttgt tcaagttatt gcggtcaaac gaaacaagca
     1141 cccagccaaa cagataccag agcaataccg gaacatgcaa caaggcaaga cgaacagcat
     1201 cacttttgtc caccggaagc agatttacat atattgccaa agcaactatc gaaattgaat
     1261 aaatcaacat ttcatgcctc ttgaacagtt tattttgcca gaaagcataa agaatcaaag
     1321 cattgaaaag aattaacgaa aggttacgca aataaaaaga atttttatcc aactgtgcga
     1381 aaaaatccgg tagttttaaa agcaatccac tgaatagtga aacgagcatc aaaaaaataa
     1441 tatccgattt cagcgcttta tggcgactat gtgaggtttc tgccgccaaa cgtacttgcc
     1501 aaaaccgata aagctccgaa tcgtagtttc ccttgttgtt tttaaaaaaa tccgaaaatt
     1561 cccttttgtg ttctctgtaa attttttcca actcttccgg agaatgcaga ttgtcgataa
     1621 tttgctgatt tatattcatt taagtaattt gaatgaaatg gttttttgta caaaacagaa
     1681 aaaattgata attaatttac tgtattgtcg ccttgtgcaa attccaatta ttttcacaca
     1741 gtggttgact atttaagaaa taaacgtaca cttttccctt aaattttaaa aaaaatcaaa
     1801 aatatactcg aataattttt tggcaccttc attcttcaag ggaaatgcaa tgctataatt
     1861 tttgtaattt tgcgacagga ttaaaactgt gattgcattg aaaaattaat agaaaaagtc
     1921 ggaattatct aaaatgattt cgatcgacgt tgcatcattc ttcaaaataa tcttcaggca
     1981 atggctaact gaatggctca tgggtttata aaagattacg aaaacaactt atcgaagtga
     2041 acaaacaaac gcataaaagg actcctcttc catcaacgaa aaagggacaa gaaatgaaag
     2101 tgagcgaaaa tgaccttttg ctcgattttt tgctgaaaag catgaaccat aagagccgaa
     2161 attcggtaaa atcattactc aaacatggtc aaatagttgt gaacggcaaa accattacgc
     2221 gatatgatta tccgttaaaa ttgggcgata taattacagt tggcaatcat caaagtatcg
     2281 ttgagaagaa tataccccaa atgcgcattc tttatgaaga tgatgacatc atcgtcatcg
     2341 acaaagaggc cggactgctt accatcacca ccggaaatgg aatagatatt acggcagttt
     2401 cgttgcttaa ggattatgta caaaaacaat ctccctacaa taaaatttac acggttcatc
     2461 ggctcgatca aatgacttcg ggagtgctgg tttttgctaa aaacagcgaa gctcagcatc
     2521 agttgcgcga caattggcac aatatggtta ccaaacgcac ttatgtggct gttgtagagg
     2581 ggaaaatgga tgaatcggcg ggaaaaatct cgagttggct gaccgaaaac aaaaaaaact
     2641 acatggtgta ttccagtcct gtggataatg ggggaaagct ggctatcaca agatataaag
     2701 ttttgcaaac atccgaccaa ttttcgctat tggaattgga actcgaaaca ggacgaaaaa
     2761 atcagataag ggttcatctc aaagatcttg ggcatcccat cgttggcgac cgcaaatatg
     2821 gagcatccac atctataggc cgcatcgctc tgcatgcgcg cgtgttggaa ttttatcacc
     2881 ctgtttcggg tgagatcatg catttcgaaa cacctgtacc ccatcttttt ttacgtttgc
     2941 tatgaaggaa gcttccgctt ataattattt ttctgagcgg aattccttct tccaacaatc
     3001 gataatgcct ctatttttca gttcttgttc taaattgatt tgaggcgtca agtacaacgt
     3061 atgaaatccg agccgttcgg ctgtttggat attttgagga ttatcgtcga tgaacagtgt
     3121 ttcctgagga ttgattcgat aacgatcgat caatatccga tagagttccg gatctggttt
     3181 tacaattttt tccgcacccg atacaacaat tccgtcaaac aaatcaaaaa aatcgtattt
     3241 agcctgagca agcggaagcg tttcggccga ccagttcgta agtccataca aattgtattt
     3301 ttctttcagc ggcttcagta atttcacatt ctcttcgaaa attccaccta tcatttcaat
     3361 ccatcgacca taatataacg caatttcgtt gctgtattcg ggatgttctt tttgtttcga
     3421 cagcgtagct tcggcgagca aacggccggc atcctgcatc agattccagt cgtatggaca
     3481 aatgtgctcg agaaaatatt ccatttcatc ttcagtctcg aaaacgtttc gatacaaata
     3541 acgaggattc cagtcaataa gtacacctcc aaagtcgaaa acgatatttt tgagaatctt
     3601 catatgacgt aagtttgaat tttatagaaa agtaaatctt aaaaattgaa aacagaattc
     3661 agttttcatt cgccctaaca gcagggttta ttaaactata aaaattggta tattagtgaa
     3721 taggaaagaa taaaaagcaa cctattaaac ggattgaaga aatttcaggt aagtatgttt
     3781 acaaatcttc ccagccatct tcgtcatcgt cgaggaagaa aggttcaaca aaatcttcca
     3841 aatcgcgacg ttcctttttt gtaggacgtc cactgccgcg tattcttcct gaattattca
     3901 gttttccgag ttcgaacaat tcgcgttgat cgggagtagt aacatcaagc agatactcgg
     3961 gcagcaattt ggcattcaat ctgttttcag tcaaggccag aacttggtaa gaaaaaagta
     4021 cgggatttcg tttcacacca accacatcgc caacacgaat cgaatgcgaa gctttcaccg
     4081 gttgcccgtg aatcagaatt ttcccttttt tacaggcttc ggcagccagc gaacgggttt
     4141 taaacagcct gacagcccac agccatttgt cgatgcgcac ttccgatttc ataaacagag
     4201 gaaattattt gttgttgtac aatgccatcg ttttttcaac cccaatcaaa cagaaacttt
     4261 ttattacttc tatggctttt tcaattctct cgggcaattg ggcagcttct tcttccgtcc
     4321 attttccaag tacatattca acttgtctgc cacgagggaa atcattgcct attccgaaac
     4381 gcaatcgggc atagtgatta tgacccagta tttcctgaat atttttcaag ccattgtgtc
     4441 cggcatccga acctttgggt ttcatacgaa ttgtgccaaa cggaagagca atatcgtcca
     4501 ccactaccaa taaatgttct acatcgattt tttccttttg catccagtat ctcacggcat
     4561 ttccgctcag attgacaaaa gtggaaggtt tcaacaaaat gagcgtatga ccttttaatt
     4621 tcacttcact gactgctcca taacgttggt cggtaaaaaa aacattggac gcctcagcga
     4681 aagcgtccaa tatcctaaaa cctatgttgt ggcgggtatt ttcgtattca cttccaatat
     4741 tgcccaaacc tacaattaaa aacttcatat acaattattt tcaaatcggt tcattattga
     4801 acttctgaaa aatttacgaa caaatcggtt gttacatttg ccgaattctt tcttttaaaa
     4861 gaaaaacgac aaaccattaa aacaaaaatt attttccgcc tgctgcagca gcataagaag
     4921 ctctggtaac cttaacctgt gcaacaacgg cattttttgc attcagaatt tcaaaatcgt
     4981 taaaggaaag acttccaacc tgaatggttt tgcccaatcc aagcgaagaa acatcaataa
     5041 ctactttttc aggaagtttg gtataaattc ccttcacttt caattttctc atttcaagac
     5101 tcaaacgtcc accggctttc acaccttcag ccaaaccttc cagtttaaca ggcaactcga
     5161 caaccacagg tttagcttca gaaatcataa agaagtcgat atgcaaaatt ttgtccgtta
     5221 ccgggtgaaa ttgaacttcc ttaacaatag ctttacattt tttcccttct atatcgaggt
     5281 ttaccacaaa tacatcagga ttaacaatca actttaaaac atccacttca gaaactgtga
     5341 aatgaacatt ttctccgttt ccgtacaaaa cacacggaac tttttcagaa cttctttcca
     5401 gcttgctggc ttttttccca aacccgttac gtaaggcccc ttttaaatca aattctttca
     5461 ttttaacttt aaattaatgt gttactcaaa atttaagcat taaaaaaaca tgtgattagt
     5521 agcttaattt cccatcacac tttctttttt caaaaaaagt gctgcaaagg tagttaattt
     5581 tttgatgtaa aacaagataa aaactaccca aattattcga ataaacctat caattacaat
     5641 caaaataaag aacaaaaaac tggtcattat tattaattta tacattatta gaaagttaga
     5701 tttgatttat cagcactctg tcggtaaaaa tattcgacag aataatggaa aattaaaaaa
     5761 aaacatttat atttgcagtc ttaaaatttt aaaataatac gtttgaaaat taataacgag
     5821 gaggaacagt tatagctaaa caattatctg tgaaacaaca aggtaagtca acagaacagc
     5881 tccatagaat taacgaaaat attaagggag taaaagaagt tcgtctggtt ggcgaaaatg
     5941 tcgaaccggg aatttatcct atacagcgag ctttagaaat agccgaagaa caaaatttgg
     6001 acttggttga aatttcagcc aaagcagatc ctcctgtatg caaggtgatt gattatcaga
     6061 aatttctata tcagctcaaa aagcgtgaaa aggaactgaa agccaaaacg gctaaagtgg
     6121 tagtaaagga aattcgtttt ggaccacaaa ccgacgagca cgattacaac ttcaagctga
     6181 aacacgcaca aaactttctg aaagaaggtt gcaaagtgaa agcttatgta tttttcaagg
     6241 gtcgttccat tcttttcaaa gaacaggcag aagtgctgct gctacgtttc gccaacgatt
     6301 tggaggaata tgccaaagta gagcaattgc cgattttgga aggaaaaaga atgacaatac
     6361 agttatcgcc aaaaaaatca aaataaagac attaatttaa ttcaattatt caatttacta
     6421 aatatttaag cgaaatgcca aagaaaaaga caaactctgg agcaaagaaa agatttacgt
     6481 tgaccggaac aggaaaaata aaaaggaaac acgcttttaa gagccatatt ttgactaaaa
     6541 agacaaaaaa acaaaaacgt aacttaaccc acagcgctct cgttcacaaa gctaatttgg
     6601 agagtgtgaa agagttatta aatttaaaat aaagtaattt ttttattcag taatcagaat
     6661 gcttagccca aagtccgttg aaaaaagacg gcgctgacat tcataaattt tatcgattat
     6721 gccaagatct gttaatcatg ttgcttcgcg caaaagaaga aaaagaattc ttaacctgac
     6781 aaaaggttat ttcggcagcc gcaagaatgt gtggactgtt gccaaaaaca cttgggaaaa
     6841 aggcttgctc tatgcttatc gcgaccgcaa ggccaaaaaa agaagtttcc gcgctttatg
     6901 gattcaacgt atcaacgctg ctgcacggct cgaaggttca tcttattccc gattgatggg
     6961 agcactacac aagtccggta tcgtaattaa ccgcaaggtg ctcgccgatt tggccatgaa
     7021 tcatcccgaa gcattcaaag ctatcgtaca aaaagcaaaa aatgcataaa ccgttttccc
     7081 ttgtaaaaaa cctttacggg gtttattgga aaattttaag taaaaaaggc tgactagaag
     7141 taggacagcc ttttttattg gatattgaaa tgaaaatccg caaaactccc ggtttcaatg
     7201 tctcggtaaa atttcaatta atagctgata aacagtaaat tttataaaac attaaattta
     7261 tacaattaac tgatattcat tattttgtac tgtaataaaa gccagttaag ccgaaatttc
     7321 attcaccagc ggttttccat ccacaaaatc ctgaatgttt tgcagcgtga tacgggcgat
     7381 ttccgaaaga gcttctttgg taaaaaatgc ttgatgagat gtaacgatta cattgttgaa
     7441 agtaagaaga cgtgccagaa tgtcgtcgtc gatcacaata tcggaatgat cctcgtaaaa
     7501 ataatctcct tcttcttcgt acacatccaa gccggcataa cctatttttt tacttttcag
     7561 tgcatctatc agagctttgg tcttaattag ctttcctcta cccgtattga taatcattac
     7621 gccttctttc attttatgga tggatttttc attaataaga tattccgttt ctttggtcaa
     7681 cggacagtgt aaagaaataa catcggaaag tccatacaat tcatctagtt gggtatatcg
     7741 aataccgttt gtttcggcaa attgcgtatc gggatacaaa tcataggcaa gtacgttcat
     7801 tcccatgctt ttcagaattt gaattacaat ttttcctatt tttcccgttc cgattattcc
     7861 ggctgtttta ccgttcattt caaatcccat taatccttcc aatgcaaaat ttccctcacg
     7921 tgttcgagtg taagctttat gaatttttcg gttcagcgta agcatcaagg caatggtatg
     7981 ctctgctatg gaatgagggg aataggccgg cacacgcaca acctttattt tgccttttgc
     8041 agcttttaaa tcaacattgt tgtagcctgc cgagcgaagt gcgatcagtt ttacaccatt
     8101 ttctactaga atatcaatta cttccgcatc aacttccgca ttgacaaaaa tacaaaccac
     8161 atcggctcct ttggtcaaaa tggcattgtc tttacttaaa tgactttttt gaaatttaat
     8221 atcgaaacca tattgttgat ttttttcttt aaaaaaaatt tcgtcgtatg attttgcgtc
     8281 aaataagata acttttgttt tcatcataat ataatttatt tttttaatgt ctgatggaac
     8341 ccacatgtct gaatatattc attcgtgttt gtgtatgcaa tgcaggtggg tttcatgttg
     8401 ttatttttat ccgatttatt tgcttagttg caatttaaac atttgttgat aagtttttgt
     8461 ttatctccgg ataagtaatt cgagaatgaa agatactttt tattttttct ttcagaacct
     8521 ttttaacagt ttcgacatct ctaaaactaa tgggagcatc ttttaattgt ccttcggcaa
     8581 tttgcgtatt gataatactt tccaccattt gatctatgct ttcttctgta tattcgctca
     8641 aagtacggga acgggcttcc actgcatccg ccatcatcaa aatggccgtc tccttagtat
     8701 ttgggagagg accgggatat gaaaacacat cttcaggggg ttctatgccg ggattatcat
     8761 tgataaagga aacataaaaa tattttgttt tacttgtgcc gtgatgtgta gtaataaaat
     8821 taataatttg attgggaaga tgatttttcc tggcaatttc tactccattg gcaacatgct
     8881 caattatcag ttttgcagct tcctcgtact gcatcgagag caaaggattt tttcctccta
     8941 tttgattttc ggtaaaaaat tcggggtttt tcatttttcc tatatcatgg taaagagctc
     9001 ctgttcttac aagtaaacta ttggcattga ttttttttgc ggcttcagtt gccaggttag
     9061 acacctgaag tgaatgctga aaagtaccgg gagcaacttc ggcaaatttc atcaacaaat
     9121 cgctattgac attggttagt tctaccaatg ttacagccga aagtagtcca aacatttttt
     9181 cgaatatata tatcagcaaa tatgcaaaca aaaggaaaag ggaactgaca gcaaaactaa
     9241 taataggata ccaatcgatt ttggaaatat ttccttctgt catgagttcg agcgaaagat
     9301 atacaacggt ataacttaaa aaaatgtata gcgcagtttg tgccaattgg gaacgctgtg
     9361 tcatatcctt taaactggag acggcaacca tgccagcagt tatttgcaac aaagcaaatt
     9421 ggaatggatt gtcggtcata aaagacacga taaaaataac gataatatgt acgaaaagag
     9481 ccgttcgaga atcgtaaaat acacgaacta ttatgggtaa taaagcaaaa ggaacaatat
     9541 agtagcttaa cgaagaatat cgaatagtca atgaagtaag ccctacaatt aggatgatga
     9601 gcaaggacaa aaacagtaaa ttattaaaat tgtccaaaat tcttggtcga aaaagataaa
     9661 tgtaaagaat caggagcgta attaaaccga ttaccaataa aattttgcct gttaaaacta
     9721 tagccgtttg ctcaaatgcc gattttctgt tttcgttttc gatttgtaaa gatcggagta
     9781 tggcataagc ttcgggagaa acgatttcac ctttatcgat aattcgttcg cctgattgca
     9841 ccatgccaac agtaagtgaa agatttttaa gcaattcgtt tttggctgtt tcggaagtga
     9901 tgctgtcata gcgtaggttt tctaccagat aattgttaat atctaattcc ttgatatttt
     9961 cctgattgct tttaactatt tcttcatatg cagtttttgg cgtgtaaatt tcatttaagg
    10021 tagcaagttg cgaaattctt cccggtaaaa tacggtagat ttttttcaca ttccattttt
    10081 gcaaattatt gtaatcgtca ttcgaaataa ttcctttcga gtaaatcgat ttcaactttt
    10141 cttcaatgaa agttgtcaaa tccggggaaa tattgcctga cctgctgctg tcgattttaa
    10201 atttatttac ctgaatatgc attacagtgg tatccagatt gaagtaaggt gtaaaattct
    10261 tgagaatttc ctttttctct ttttcaattt gctcattgct tttgtatatc ggaaaatcaa
    10321 aaggtgcagt caaaagttca tacgaccaag gtttaccttc ttcaaactga taattgaaag
    10381 tgctttcatg cggataaagt tctacaatta ttattaccaa agcgaaagca agcaaaagtt
    10441 ttaaaacggt ttcaaatttc tttttgttga ctttcagttt catgactttt tgttttttga
    10501 tttactactg taaaaatctt tatttctcta atcagcaaaa atttgtggaa tgttcagatt
    10561 ttgcttgtta ataattcctg ttttttcatc agaatataat taatttttta aaggtctgat
    10621 ggaatccaaa tgtcaatatt atattcattc gtgtttgtgt atgcaataca tgtaagtttc
    10681 atttaaaatt ttgttttgta aagatttcga cgccgtaaaa aagctaaaaa ttaaaaaaac
    10741 acgccttatg cttatcagtt ttatttactt gtgaatcaaa aattaagaat atgagccact
    10801 atagaaaaaa gagtgaaaat accaaaagaa ttgggttgat agcattatgt tgcatgtaac
    10861 atgtaaaata tagttattca gatggtaaag atacaacaaa aaattatttt gaaggccgaa
    10921 tttgaatcaa cgataaaatg ttttcatgcc accaatttga tgcgatgttt aatctgtggg
    10981 taagagatat ataattttac attgttgtcc ggtaaacatt tataaaaatt agtgaaaacc
    11041 aatatagaaa atgtcttact ataatatcgc ccctaatggg gcttagattt tgtgggttga
    11101 atttttctac cataatttcg ctgcaatgca gctttctctt aagtcccatc gggacgatat
    11161 tatggtagtc aataagttac aaatatcatt agagctccgt aggagcgaca ctatttgctc
    11221 ctttattttt atcggacaac attaataatt ttaaaaattc ttatgatgaa tggagcccgc
    11281 tttaatttat attgaaaagc agatgttttt caagatgtga ttggtaattt ctatcagaaa
    11341 ggaacctcgc tattattatt tttcagcaaa ttatccagtg caggcgagcc agaagtattt
    11401 tcataaaaat taccctcagg cacattcatt ttggaagaaa taacctgata gttttcgttg
    11461 aaaatatcat cggagtcgat gtcatcaaga tttaaaaact tggcaaattc ttttttgaat
    11521 tttaaatgga tggtatcggt agcaccgttt cggtgttttg cgacaataat ttcggctatg
    11581 ccaatcatgg aattgccatc tttgtcggta gtcaagttgt agtattcggg acgatgaata
    11641 aagcagacca catcggcatc ctgttctatg gcacctgatt cacgcaaatc ggacaattgc
    11701 ggacgcttgc catcaacccc gcttcttcct tccacacttc gattcagctg cgaaagcgct
    11761 ataatgggaa tatccaattc tttggcaagg cttttcaacg agcgggaaat ggtacttact
    11821 tcctgttcgc ggcttgaaaa tcgcatttca ctggcattca tcaattgcag ataatcgata
    11881 aaaatacact gtactttgtg ctccttgacc aaccttcgtg ccttgctcct caattcggaa
    11941 atcgaaagac ttggcgtatc gtcaataaaa acaggtgcat caatcaaatc ttttatgttt
    12001 ttatcgagtt tatcccattc ataagggctc aaatcgccat tttttatttt tacaccatca
    12061 atttcgcaaa cattcattat caaacggttt gctaattgta cactggacat ttcgagcgaa
    12121 aaaacgccaa ccggaacatt gtagtcaact gccatatttt tagccataga cagaacaaaa
    12181 gcagttttac ccattgcagg acgagcagca ataataatta aatcggactt ttgccagccg
    12241 gaagtaattt tatccaaact gtgaaaacca cttggtacac cacttgttcc ctgtttattg
    12301 gcagcagctt gtatgcgctg aatggcttca tcaatcaccg gtttgatctg aataacctcc
    12361 ttttcgagat tacgctgcgc aatttcaaac aacatttctt ccgactcttc tatcaaatcc
    12421 tctacatcgg ttttgtcgtc gaaagctttg gtctgaattt cactggaaat acgaataagt
    12481 tcccgagcta aaaatttctg caggataata tgcgcatgat attccaaatg tgcagccgaa
    12541 gaaacttttg cagtcaacaa cgtaatataa tatgggcctc caatttcttc gagcgtacca
    12601 tcccgacgca actgctccgt tacagtgtgc atgtcgatag gttcctgatg caaagccagc
    12661 gtcataatgg cttcataaat tttctgatgg gtgggtttgt aaaaacacac cggtttcaaa
    12721 atttccgaaa ccagaaaata ggcatctttt tcaatcataa tcgctcccaa aacagcctcc
    12781 tccagctcaa gcgcctgagg tggaagtttt cccatttcat ttggagcaat cactgtgggt
    12841 gcactatttt gttttttgtt gtatgttttt ttttcggcca ttggtatttt ctatatgttt
    12901 atgtaaagtt tgaattaact tttcaaaagt acaattattt tccaaatttg agagaatttt
    12961 ttgtcaacag ctgttgataa gttttatagg aaaacaccaa ataatcctga aaaatatgac
    13021 cttaagctgt aaaaattttt atcgtttcat ctgttttttc cataaaaagt tcaaacacca
    13081 aagatttgac aggcctcaca gtgtgagaat tgaaaattat catgtaactt tgcatgaaaa
    13141 ttgaaattac ttcgtacaca tgaatataga acttagcgat caggaacaat acagaagaca
    13201 aagtctcagg gaattacgaa atttaggcat agagccctat cctgctgcac tttatccaac
    13261 caatgctttt tctacagata taaaatcgaa ttttgacgaa aacaatcccc agcgcatgga
    13321 agtttgcgta gccggtagaa tcatgagtcg gcgcatcatg ggaaaagctt cgtttgtaga
    13381 actgcaggat gcaaaaggac gtattcagat ttatattaca cgggatgata tcaataccga
    13441 agcacaaccc gaaatgtaca acacagtttt caaaaaactg ttggatattg gcgactttat
    13501 cggtatcaaa ggttatgttt tccgtaccca aacaggtgaa atcagtgtcc acgcaaaaga
    13561 attgacggta ctttccaaat cacttcgtcc actgcctata gtaaaatata aggatggtgt
    13621 agcctacgat gcttttgacg atcccgaaca gcgctacagg cagcgttatg tagatttagt
    13681 agtgaatgaa ggagtgaaag atatttttct taaacgaaca aaagtttacc aatccatgcg
    13741 cgaatttttt aatactcgcg gttatgtgga agtggaaact ccgattttgc aacctatccc
    13801 cggtggcgct gctgctcgac ctttcatcac ccaccacaat gctttggaca tgcctctgta
    13861 tctgagaatt gcggatgaac tttatttgaa aagattaatt gtgggcggtt ttgaaggtgt
    13921 ttatgaattt tctaaaaatt tcagaaacga aggtatggat cgtacccaca atccggaatt
    13981 tactgctatg gaaatttatg tggcatataa ggattatttt tggatgatgg actttacgga
    14041 acaaatgatt gaaaaaattt gtctggatgt aaatggaacg accgaagttc aggtgggtga
    14101 taaaacaata agcctgaaaa cgccttacaa gcgtatttcc atgattgatg ccattaagga
    14161 aaatacaggt atcgatattg aaggaatgga tgaaaatgat ttgagaaaag tatgtgaaga
    14221 tttgcaaata gatgtagacg aaatgatggg aaaaggaaag cttattgaca aactttttgg
    14281 agaaaaatgc gaagccaatt atgttcaacc aacttttatt acggattatc ctttggaaat
    14341 gagtccgttg tgcaaacgtc atcgcaacaa tcccgaactt acggaacgtt ttgaattgtt
    14401 ggtgaacgga aaagaactgg ccaacgccta ttccgaactc aacgatccga ttgaccagtt
    14461 ggaaagattt caggaacagc ttcgactgag tgagaaagga gatgacgaag ccatgtttat
    14521 tgacatggat tttgtccgcg cattggaata tggaatgcct cctacttcag gaatgggtat
    14581 cggtatggac agactagtga tgctgcttac cggtcagcaa gccattcagg aagttttgct
    14641 ttttccgctg atgaaacctg aaaaagttca aaaagtcgat accaaagaaa aattcatgga
    14701 attggatatt cctgaggcat gggctgaagt acttcaaaaa atgggaataa aacaggttga
    14761 ccaattgaaa gatttgaatc ccaataaatt gcatcaggac atttgcggat tcaacaaaaa
    14821 aaataaactt gcattgacca atccttccaa agaagaagtc agccagtgga ttgacaatgc
    14881 agcgaaaaaa taatcattca gaatcgtttt acagtcttat attgttatct ttagtttact
    14941 tgtatttttt agagtttaat tctttgtatt gagttggttt ttaaattaaa aaaaattaat
    15001 gttgtccgat aaaaataaag gagcaaatag tgtcgctcct acggagctct aatgatattt
    15061 gtaacttatt gactaccata atatcgtccc gatgggactt aagagaaagc tgcattgcag
    15121 cgaaattatg gtagaaaaat tcaacccaca taatctaagc cccattaggg gcgatattat
    15181 agtaagacat tttctataat ggttttcact aatttttata aatgtttacc ggacaacaat
    15241 gaaaaaaatc ttttttatga tgctcaaatc ttatgtttga aattgttttt gaactttctt
    15301 ctttggccga tactgcggtt ttttatggaa taaacaattc caatatgcag ttgataaaaa
    15361 atttacatcc caatttacgg attgtggcga gaggaaatgc tattaaaata attggaaatc
    15421 agcaggatgc ggacaaacta attgttaatt tgcagaagct tgaaaaattc agcatcgaca
    15481 acaatcaact tactgaaaaa aatatcatcg aattaataaa gggtgaaaat ccgaaggata
    15541 tttcgtttca aaacctgatt atgcacggtg ttggaggaaa atcaattttt gcacgcacca
    15601 aaaatcagca acgtctagta gaagaatttg aaaaaaatga tttgctcttt gccatcgggc
    15661 ctgccggttc gggaaaaacc tatacggcaa ttgctttggc cgttcgggca ttgaaaaaca
    15721 aacaaatcaa aaaaattata ttgagtcggc cggctgtaga ggccggcgaa aagttgggtt
    15781 ttcttccggg cgatatgaaa gagaaaatcg acccctatct gcaaccgctt tacgatgctt
    15841 tgcaggatat gattaccccg ctcaagctga aagagtacat ggaaaacggt accatacaaa
    15901 ttgcaccgct tgctttcatg cgtggacgaa ccctgagcga tgccgtcgta attctcgacg
    15961 aagcccaaaa ttctaccatc atgcagataa aaatgtttct cacccgcatg ggactgaata
    16021 ccaaaatgat agttaccggc gatattaccc agattgattt gcctcgcgac cagaaatcgg
    16081 gtttgattga tgcactgaat ttgcttggca acatcaaagg cattgcccgc atcgattttg
    16141 atgtcaacga tattgttcga catcggctgg ttcagcaaat tgttcaggct tacgacaatc
    16201 gggcaatgaa cgaaagacaa aaaaatacta aagaataaat aaccgaacga atcatttgtg
    16261 attttttcct ttttttcttt aaaactatat caaccctcaa aactaaaaat tatgaatacc
    16321 cttgttcgca ccgattttca tttcgaagga cagaccaatg tttatcatgg taaagttcgc
    16381 gatgtttact ttatacagga tgacctgctg gtaatggttg ctaccgatcg tatttcggct
    16441 ttcgacgtta ttttgcctaa aggaattccg tataagggac aaattttgaa tcagattgca
    16501 gccaagtttc tcgacctcac agccgacatt gtttccaatt ggaaactcgc ctgccctgat
    16561 ccaatggtaa ccgttggatt aaaatgtgaa ggttttccca tcgaaatgat tgtgagaggt
    16621 tatttgtgcg gcagcgcttg gcgtgcttat aaaaatggcg ttcgagaaat ttgcggagtt
    16681 aaacttcctg aaaacatgcg cgaaaatcag cgttttgaaa cacctattat tacgcccact
    16741 acaaaagccg aattgggaac gcacgacgaa gatatttcca gacaggaaat tctcgcaaaa
    16801 ggtttgttga ctcccgaaga atacgatttg ttggaaaaat atgcactggc actttttgaa
    16861 cgtggcaccc aagtggctgc ggaaagagga ttgattttgg tagataccaa gtacgaattt
    16921 ggaaaaagaa acggaaacat ttatctgatt gatgaaattc atacacccga ttcttcccga
    16981 tatttctatg cagaaggtta tgaggaacgt tttgaaaagg gtgagccaca aaaacagctt
    17041 tcgaaggaat ttgttcgtga atggttgatg gaaaacggtt ttcagggaaa agaaggacag
    17101 aaagttcctg aaatgactga tgaaattgtc aacagtatca gtgaaagata tatcgaactc
    17161 tatgaacaaa ttacgggtga gcctttccag aaaaccgata cacgaaatct tgccgaacgc
    17221 atcgaaagca atgtagtgaa atatctgcaa agcttaaaaa aataaaccta cgcatcggta
    17281 ggccgataat aaagaatcca ctctttaact gaaacctttc gggctttaaa gagtggattt
    17341 tttattggaa atttccattt atccttgttt ttcaccggtt atccgttcga gccatttcaa
    17401 catggccagc atggtgaaca tggaaatcag gcaagccccg acaaaaacgc tccaagtcat
    17461 ccaaatcgga atgtttttgt acatcagtgc accaagaaaa agcaactgat ttcccagtgc
    17521 cgtagcagcc agccaaccac cttgcatgat tccctgatat ttttgtggtg ccacttttga
    17581 aacaaaagaa attcccagcg gactgataaa cagctcggca accgttaaaa tgaaatacgt
    17641 tcccaacagc agtaatggcg taacgcgttc agcatcgggc aatcctcctc ttaaagtaat
    17701 ttcttctttc gaaggcaagc ccagcgaacc cagcgtcatc acaacataag ccaaagcagc
    17761 tattcccata ccaatcgcaa tttttcgtgg cgttgaaggt tcttttccct tgctgcgcaa
    17821 gtaaccaaac aaagccataa ccaacggggt gagaaatacc acaaaaaacg ggttgatgga
    17881 ttgaaaaatt tctgctccct gcagcgtagt aaatccaaga ttcacggaaa ctttgctcag
    17941 gttggtataa tctttggcaa aaaaagtcag ggtcaagccg ttttggtgga aagagaacca
    18001 aaagaaaatt accactgcaa atactgccaa caaggcatac aaacgttgtt tgatttcttt
    18061 ggcatccatt tctttaccgt tttggtccaa agttactgtt gcggttgaag ataaattacg
    18121 gggttgagga aaggattttt tgtttgttac aaaaattatc aacgaaacaa acatggcgcc
    18181 aatggcaacc ataaaagcat aatgaaaacc ggtggtaaac aaattcagat actgatctgc
    18241 aaaagaagtc atatttgaac ctgtgtaacc ggcattaaca gccaattcat gcatccgttg
    18301 agctgcttcg ggcgtaatga tgccattcaa ctggtcatga cacaaagcag gaagatcagg
    18361 attataagca aagccatgag agtgcaacca ccagtttctc acgccaatag cagcaaaagg
    18421 agcaaatatg gctcccacat taatgaacat gtaaaacagt gaaaaacccg aatcacgcat
    18481 tttttcgtac ttcggattat catacatttg ccctaccaat gcctgcaaat tccctttaaa
    18541 aaatccattc cctaaagcta tggtcaacaa tccgaaacag gttaatccga gaaaaaaccc
    18601 gtatctgttt tgtggaacag gagtgggtgt aggaagcgct actaagaagt aaccaattga
    18661 cattaatatt agcccccaca agatggttct tttataattt cttgttttat cggctatcaa
    18721 tcctccaaca aatgccaaaa tataaattga aaaataaaaa acagaataaa ttaagcctgc
    18781 ttttgtttca tccaatccgt attttgccat caaaaacaac gacaaaatgg ccatcatggt
    18841 ataaaatcca aaacgctccc ccatattggc aagcgaagca gctataagtc ctttgggatg
    18901 atttttaaac ataaacttga tctcatttta gtgaaactat cagaagaata atttataaaa
    18961 atgcaaaagt gaaaaattta tttattccaa tccatatcct gaccaaaaaa attaaattgt
    19021 tttaactaat tatttcatct gacaacaatt caataaaaat taagaatggc ttgacaaaaa
    19081 cgacagaatt tgttgaaaca tttcttcatc cgtaccactg acaaccaacg ctgccttacg
    19141 ataaaaaggt tcccgttccg acaagttttg tcgaataaaa tccaataatt ctttaccttc
    19201 tcgctgagcc agcaagggac gtttattctt gtgtgaaagt tcgagccgtt cctgtaattg
    19261 ttcagggctt aaacataaat aaacggttaa gccataagca ttcattcgtt ccatgttatc
    19321 gtaaaagcag ggcgctccac caccggttgc aaccaccaca ttttgttcgg tggatatttt
    19381 atctaaacat tctctctcca atctccgaaa ttctttttct cctttttcag agaaaatttg
    19441 cgaaacggac tgttgcattt tttcttcaat cacatgatcc atgtccaaaa acttataacc
    19501 caaccgttca gccagttgct caccaatagt ggttttgccg gctcccatat agccaattaa
    19561 aaatattcga ttgtctttat tagattgcat ttttataatt ttcttttatc gcttttgtcc
    19621 gcaaagttaa atgtttaccc tcaacgaacc taaaattgtg catttattct gcaattagcc
    19681 ctaaaaaatt gctaaatcaa tgaaataaaa aaaccctgca agaaaatata tcttcacagg
    19741 gttaaatatt taaaaatgaa aaacaattaa tcattcttcc acaactgcag caggttttat
    19801 gggcgttttt aatttaaaag tagtaacgtt gctcccatat acatcatcaa actgagcgtc
    19861 ggaaaccaat gaaaattgaa gtatttcatc attggttgcg cttttagcag caaaatgaaa
    19921 cggaaaatag acggcattga acccactcag aaaaacaaat tttccctgcc cgtaatcgga
    19981 attggtgtgt gaaaaaacgg agtcgaggct tgctaccgga ggaaataaaa aatcaccggc
    20041 actgtcaacg gtttttgtca aacgaaatga aaccagatta tttgtatagg cgtttaatcc
    20101 gagttgaaaa aagacagtat cacctacctg aatactatcc aaaagataaa ccccagattc
    20161 attagtagtt ttaatgttta acgtatcacc atgttgggtg taggggaaag ctaaaaattg
    20221 aatagcagga gtgtaattgg aattgccatc aatattgcac ccggttaata aaacaaccgc
    20281 catgaacgat agaagaacaa actgtttttt cataatattt tgaaatttga agtgaatgaa
    20341 aggaagtaac aaaaaacata ccaatcaacc tgaaacatca aatccaactt tgtcggggtg
    20401 acaagactcg aacctgcgac ctctacgtcc cgaacgtagc gcgctaccaa ctgcgccaca
    20461 ccccgccttg attattcggc tgcaaattta catcattttt tgatatttcg agcatgaaaa
    20521 taagctaaaa ttattcacag atggatatcc gcataatttt aaccattatt tatgtgtttg
    20581 tatattagtt atttaacaat tttttgaaac tataaattag gagaataagc gttgaaatta
    20641 ctacctttgt gaaaaataat taaattcatg tttacagagg ctattcaaaa ttatctgcaa
    20701 cgacacgact acaaaagttt ttatcccaaa gccgtatttt tcgatatgga tggagtgctg
    20761 atcgactcca tgaagaacca ttccagagca tggatgcaag ccatgaaaga atcaaacctg
    20821 ccttttaccg aagaagatgc ctacatgcac gaaggttgta ttggtgctac caccatcaac
    20881 gatactttta tgaagcatct gaacagggaa gctactgaag aagaacaaaa acaaatttat
    20941 tcagcaaaat caagaatctt tgaatcgctt gagcccatcg aaaaaatgcc ttttgcttat
    21001 gaattgctca aaaagttgaa atcggaagaa atgaaaattt atgttgttac cggctcggga
    21061 caacccaaat taatcgacag tctgaatacg cttttccccg aaattttcac aaaagaaacc
    21121 atgataacgg gtttcgacgt aaaagaaggc aaaccatcac ccgaacccta tttattggcg
    21181 ctgaaaagat ccggatttca accctgggaa acgatagttg ttgaaaatgc accaatggga
    21241 attttatccg cttcaaaagc cggcttgttt actttagttg tcaatacagg cccgtttgac
    21301 gatgatttcc tttatagcta tggagctgat ttggtactcg acagcatgga acatttgtac
    21361 aatgaatggg acaatttccg taaatcgtgg atttgaaatg aaaaagacag gtttggaact
    21421 tttcctttct cccgtttttc tttccctgat tttattcttt tcttcaaccg ttatttttgc
    21481 cggagaaact gattctgctt cctttcatcc ccaaatagaa ggaacgatca gggcgaagta
    21541 cgaatacaac accagtttga acgcttcccg ttttcaggta cgtaacgctc gttttagcgt
    21601 caacggaaat tttaattccc tcgtttccta caaaacggaa attgatcttt ccgacgaagg
    21661 tcaaaccaaa atgctggatg cctatattcg actgcaacct caaaaatggt tcactttcac
    21721 catcggacag caaaaaattc ctttcagcac cgataatctt cgtcctcctc atttgctgta
    21781 ttttgccaat cgatcgttta tcgggaaaca actcaccgca ctcagagatg tgggcactac
    21841 attaagtttt gtaaataata aaactttacc ttttgaattt ataaccggtt tgtataatgg
    21901 cgagggtctt tataatcaga aaatttggca aacttcattc agttatgctt ttagattggt
    21961 tttacatcct tgcgaaaatc tacatttttc attaaatcat aattctattc gtcctgaaga
    22021 aatgcggatg aatctatatg atttgggagg tattttacag ctcaaaaatt ggcatctcga
    22081 aaccgaattg ttctataaaa cctatcaaca gaattttttc cctaccacca aaggtttttt
    22141 tgttttttcg agttatgata tatatctaca taataaagaa agtattaaaa ttattcgtcc
    22201 acttatacga tacgacagta tgacggacaa taacaaaggt tacaaatcgg aaaacggaag
    22261 ttatgaatca gacgacatgg caagatcaag aattacaggc ggaataactt tgtgtcttga
    22321 caaacctttt ttaaatgata ttcgattaaa ctatgaacag tatttttata aaaaaggcat
    22381 tcaaaacaat gacaataaat ttgtggtaga atttgtactt cgtttttaat aaaaaagcat
    22441 taaatttgca atacgttttt tattttctcc aaaaaaacaa aaatcaggaa gaaaatcaat
    22501 tatgcaagta ataaaatatc cggccagaga attttggtca acccttctct cccgccccgc
    22561 cattgataga aaaagtttat ttgaaaaagt tggaagcatt gtttcagagg tgaagcaaaa
    22621 cggagataaa gcagtcaaaa aatatacctt acagttcgac aaagttggca ttgaagaact
    22681 tgaagtgagc caagaagaaa ttgaagaagc atccttgcag attccaaacc agttaaagga
    22741 agccatagaa atgtgcagac gaaatatttg gaaatttcat tctgcacaac aacacgattt
    22801 accggaaatt cagactacac cgggagtggt ttgctggcaa aaagccatgc ccatagaaaa
    22861 agtggggtta tatgttcccg gaggaacggc tcctttgttt tcgaccgttt taatgttagg
    22921 cgttcccgca caaattgccg aatgcaagga aattgttctt tgtacacctc ccgataaaga
    22981 aggcaaaatt catccggctg ttctttatgc tgcaaaagtt gcaggcatac acagaatttt
    23041 caaaattgga ggaatacaag caattgcagc tatggcttac ggaacagaaa ctgtaccgaa
    23101 agtgtataaa atttttggac cgggaaacca gtatgtaact gctgccaaac aattgattgc
    23161 gttggatggt attgcaattg accttccggc aggtccatcg gaagttatgg tgatagcaga
    23221 cgaaagcgca cgaccctgtt ttctggctgc cgatttgctt tcacaagccg aacacagtgt
    23281 cgacacccaa tcaattctca ttactgttag cgaaaatctt gtagaaccgg ttcttttgga
    23341 aattgaggaa cagctcaacc gcattccaaa aaaagaaatc gctctcaaag ctttggaaaa
    23401 tggtaaaata attgtgctgc aatcgttgga agaagcagta gcaatggcga acgaatatgc
    23461 acccgaacat ctgattatct ccactcgtta cagtttggga gttgcatcca atatcagcaa
    23521 tgctggctcg gtatttctcg gacactatac gcccgaaagt gccggagatt atgcttcggg
    23581 taccaatcac accctgccca ccaacggtta tgcaaaagcc tacagtggag ttaatttgga
    23641 cagttttatg cggaaagtta cttttcagca aatttctcgc gaaggacttt ccaatttgag
    23701 taatgccatt attttgatgg ccgaaaatga aggtctttat gcccacagta acgccgttaa
    23761 agtgcgattg gataaagaag aatcagagga aaaacccgaa aaccggcttt gttaatacat
    23821 gcattacact tttcttcgtt tgaacaacaa atttagaatc aaaaaaatca catgataaaa
    23881 ttagattaga atgaaaataa atgaattagt aagagagaac attcgtaata tgcagccata
    23941 ttcgtgtgct cgcagcgaat ttaagggcga agcttccgtg tatcttgatg ccaacgaaaa
    24001 tccgtataat tcacctttca atcgctatcc tgacccgctg cagactgagc tgaaaaaaga
    24061 aatttcaaaa attaagaaag ttcccgttga aaatattttc ttgggtaatg gcagcgatga
    24121 agcaattgat ctcttgtaca gagctttttg tgaacccaaa attcacaatg tggttgccat
    24181 tgaacccagc tatggtatgt acagggtttg tgctgaaata aataatgttg aatacagacc
    24241 ggtcttgctt gatgaaaatt ttgatgtgga agcttataaa ttaatggatg ccagcaatga
    24301 caaaacaaaa attatatggc tatgttcacc caacaatcct accggcaaca gtttgaattt
    24361 ttcggaaatc aacaaacttc tttcatggtt cagaggaatt gtggtaatcg acgaggctta
    24421 tatagatttt tctacaaaaa aaagtttact gaattacctg actcttttcc cgcaattgat
    24481 agtattgcaa accatgtcca aagcgtgggg aaatgcagga atacgtttgg gaatggcttt
    24541 tgccgatccg gaaataatcg ggatactgaa taaaatcaag tatccttaca acatcaatta
    24601 tcttacccag caacatgctt tacaagcgtt gcaaaatcct atgcaggtag aaaaatgggt
    24661 agcattaatt ttggaggaaa aagaaaaact gataaaagaa cttcagaaat taaaattggt
    24721 agaaaaaatt tatccttctg atgcaaactt tattttggta aaagttgaaa atgccaatgc
    24781 aatttatcga tttctcattc aaaacgggat tgtcgtacgc aacagaagta atatgccttt
    24841 gtgtaacaac tgcatacgca ttactgtagg aaccccagaa gaaaacaaag cattaatcga
    24901 tacactgaaa aaatttcacg tgtaaaattt tactctccat tacgaaaaaa gcgcttgaaa
    24961 ggaatccttt caggcgcttt tttgtagcgg gaacgagagt tgaactcgtg acctccgggt
    25021 tatgaatccg acgctctaac caactgagct atcccgcctt tttgtttttg cggtgcaaag
    25081 atagcttttt ttaataattc cgacaaccct ttgcaataaa aatctatttt ttcaatctta
    25141 taattcggta gccggttcac cctttaacat tactaaacaa gctattttaa caatgaaact
    25201 caagcaaaaa tattgttccc gaaccatgat acagttattc caagttatac attctattta
    25261 tctcattatc agcaataaat agataagcat agaaaactat tttgcaaatt tcgggttgaa
    25321 ataaaaaaag aattgatatt caatcaatgc ggttttttcg tgggtattga tttattgtaa
    25381 ccagcagatg attaaatttc ccattgttca caagcatcaa tgagttcatc aaacgatttt
    25441 acggaagttt gtttttttat ttcattgatt tgattttcca tagccatttc ataagtttct
    25501 tcctccacat cacgaataat tcccatagct atgggcattt ctggaccttc catcaatgcc
    25561 agtttggtat gtaaaaaagt atctttgcac ttggcatcat gaaccagaat atccttatct
    25621 gtaattccat tttcaccaat cgttacgact tttagttctg atttttccaa aatcaatcct
    25681 ttatctttat tttttccgaa aatcatgggc ttaccatgtt ccaacacgac aattctatca
    25741 tctctggttt ctttttcggc caaccatcca tgcgcaccat cattgaaaat cacgcaattt
    25801 accaaacatt ctactacaga agttccttta tgaagggcag cagccttcat gaccatttga
    25861 gaagttttca aatcgacatc caataatctt ccaaaaaatg ttccaccggc tccaaaagtc
    25921 agttcagccg tacgaaaagg tcgctcaact gtgccataag gagaagattt ggtaacaaaa
    25981 ccttttttgg tagtaggaga atattgtcct ttggtaaggc cgtaaatctg attattaaaa
    26041 agtagaacat taatgtccac atttctcctt acgctatgaa taaaatgatt tcctccaata
    26101 gccaagcaat caccatcacc ggtaacttgc caaaccgtaa gcgcaggatt ggcaacttta
    26161 acaccggttg ctacacaagc gcctcttccg tgaatggtat gaaatccata agtattggtg
    26221 taataaggga gacgggaaga acaaccaatt cccgaaataa tgaccgtttc gtgtggtgaa
    26281 acacccaact ctgccattgt cttgaccaga gcattgaaaa tggcataatc tccacaaccg
    26341 ggacaccagc gaggttgctg gtctgtttta aagtcggcag gcatatattt tgtctcttcc
    26401 ataatttcta tgattttttt ggatgcatga aataattata gcagcgaaat gaaatgattt
    26461 tccaattctt ccgttttaaa aggctggcct tgtattttgt tgaattgggt aaaggttttt
    26521 ccgggaattt tggatcgcag atacgaagca aattgaccgg tattcagttc gcaaacaact
    26581 attttttcgt attttccaat aacattttca gtattggcgg gcagcgggaa aagataagta
    26641 aaatgagcca acgctatttt atatcctttg ttgttcaagt tgtccacagc acttttcaaa
    26701 tgtccgtaag ttccacccca accaactacc aacaaatcgg catctgtatt tccttcgatt
    26761 tcgagttctg gaattacatt tacgatattg tcaaccttct gttttctggt taaaaccatt
    26821 ttttcatgat tttcgggatc ggttgaaaga tttccggttg tataatcttt ttccaaacct
    26881 ccgttgcaat gttgaaaacc tttcattccc ggaatactcc aataacgaat cttggtatct
    26941 tcgtttcgag tggtaacctt gtagttttcc atattcgatg gaacaaaatg aggatgaatt
    27001 tctggtaagt cggcaagttt tggcaagcgc caaagagaac ttccatttcc aataaaagca
    27061 tccgtcagca gaataacggg cgtcatattt tccaatgcaa ttttgctggc cataaaagca
    27121 taatgaaagc acgagtcggg agttggagct gccaaaacag caagcggact ttctccactg
    27181 cgtccatata atgcctgcaa caaatcaccc tgttcggttt tagtaggcat acctgttgat
    27241 ggaccactgc gcatgacatc tacaattacc aaaggcagtt cagccattac agccaaacca
    27301 attccttctg ttttcaacga catgcccggc ccggaagtac ttgttgcagc caagcagcca
    27361 gcaaaagaag cccctattgc cattgtaatg cctgcaattt catcttcggc ttgtacggtt
    27421 ttaacaccaa tacctttttg caaagccaat tcttgcatta tgtctgtggc cggtgtaatg
    27481 ggataacttc ccaaaaaaag ctgaagtccg gctttttcgg ctgcagcaat caatccccaa
    27541 gccgtagctt tgttgccgtt aatacttgtg tatttgccct ttggcatttc gtgcgatttt
    27601 tctatcacaa aattggatac ggtcagatga agattttcgc cataattgta tccatctgta
    27661 agagccttaa tatttgcttg aagaatttgc gttttttttc tgaatttatt ttccagaaaa
    27721 tgaatagctt tatccagcgg tcggttgaaa agccagcaaa ccagccctaa agcaagcatg
    27781 ttcttgctgc gcaatacggt tttgttatca aaaccaaagt cttcgagact gcttttggta
    27841 agtgttgtca gtggaacagg aataagctga atggtgtctg aaagagaaag ctcttcaaaa
    27901 ggattatcag tggaaaaatt ggcttttttt agatcagatg gctgaaaaga atccaaatca
    27961 aaaataacaa tgctgccttt tttgagattt tttgcgttca ctttcagcgc agccggatta
    28021 agtgtcacca agacgtctat ttcgtctccc ggagtgtaaa ttttgttggc cccaacatga
    28081 acctgaaatc cggaaactcc acccagagtg ccttgaggag ctctaatttc ggaaggaaaa
    28141 tcagtaaagg tagatatttc gtttcccaat tctgccgaaa gttgagcaaa caaatttcct
    28201 gtcaactgca tgccatcacc cgaatcgcct gaaaatcgta taacgatatt gtccaattga
    28261 atggtttgtg ctttattcat ttttgaaaat agtgaatgaa ttaaataata tcttacaaaa
    28321 acttttttaa ctatgtgtat ttaaaaatcg gcattttttg gcgtgcgtgg aaatggaatc
    28381 acatcacgaa tattggacat gccggttaca aaaagaagaa atctttcaaa tcccaatcca
    28441 aatcccgaat gcggcgcatt gccaaattga cgtgtttcga gataccacca aatatcttta
    28501 tcgggaactt ccatttcctt tgctctggct ttgagttttt cataatcgtc ttcccgttgc
    28561 gatccaccaa tgatttcacc aattttcggg aataaaacat ccatagcgcg aacggttttt
    28621 ccatcgtcgt tcagcttcat gtaaaatgct ttgatatttt ttggataatc ggtgagaata
    28681 accggttttt tgaaatgttt ttcaacgaga tatctttcgt gttccgattg taaatcaatt
    28741 ccccaaccta ccggaaattc aaatttttgt ccttttgcca tggcgtcttc cagaattttt
    28801 attccttcag agtaagttag ccgaacaaaa tcattttcta tgacaaattt cagacggtca
    28861 atcaattcgg gatcataacg attgttgaga aattccaaat catcactaca cttttccaac
    28921 acccaacgca cacagtattt tacaaagtct tcagccaatt gcatgttttc ttccagttca
    28981 ttgaatgcca cttctggttc gatcatccaa aattcggaca aatgtcgggg agtattggaa
    29041 ttttcggcgc ggaaagtggg gccaaaggta taaatggctc ccaaagccaa agcccccaat
    29101 tcgccttcca actgaccgga aactgtcaac ccggtttgtt tgccaaaaaa gtcattgctg
    29161 taatcgatac atccgttttc agcttttggc agatcggaaa gatttaatgt ggtaacctga
    29221 aacaattgtc ctgcaccttc acaatcagaa cctgtaataa taggtgtatg aaaatagaag
    29281 aatcctcttt catggaaaaa agtatgaatg gccatagcca tgtgatgccg aatccggaaa
    29341 actgctccaa aagtattggt tcgtgggcgc aaatgtgcaa tttcgcgaag aaattccatt
    29401 gtgtgttcct ttttctgcaa aggataggtt tcaggatcgg ctgttccata aattacgata
    29461 tcttctgcct gtatctcaac gctttgtcct tttcccagcg attgggccaa tttcccggtt
    29521 acacctatac aggcaccggt ggtgatcggt tttaaaaaat cgattcccaa tttattgttg
    29581 tctgctacaa tttggatatt gtgtacggta gaaccgtcgt ttaattcaat aaagcttaca
    29641 tttttatttc ctcttcgggt gcgtacccat cctttaacgc aaatatcttc accgaagtcg
    29701 atttttttta aagcatcaac aatttttgtt cttttcataa gttcgatatt tttattttcc
    29761 tctgaaattt tttgttaaca tcggagaaat caattctttg tcccttcttt taccctttaa
    29821 cttttttaca aagatgcaaa attacaaatt tcaaacgaaa aaccagcctt gtcgacataa
    29881 aaaaagcgtc tgaaatacta tgatcaggcg ctttttaaat tatattattc aaaaaatcaa
    29941 cgttttacgg gctctatatt tcccatttta ctcatggcac aacggaaacc tatatcgttg
    30001 gaacattgat cttgatcaag ataacgacga gtggaaggat ttaaccagta tattcggtct
    30061 ttccacgaac cacctttgta aactctagtt ttgtcggaaa tttgaggagc caaaacatct
    30121 gtaggatcaa tttttttcat attggtaatg gctgcaatat cggaagaatc agcataaagc
    30181 tgaaaatcgg tcgaaatttt tgaagtaaga tctccatcct tataattacg cacatcatct
    30241 ccttccagca ccgtagtggc aattctaccc aaagagtcga ttttaaaaat tttattgccc
    30301 acttcatctt ctttttcaac aataggcgtc aaatacatat ttcctctaaa agaattgtat
    30361 tcggcaacat cttcaaaact ggtagcgcgg taaacatcaa gaacccattc gtttacatta
    30421 ccggccatgt tgtaaagtcc aaaatcgttg ggataatatg aattgacggg agccgtgatg
    30481 gtagctctat cgttcaggct acctgccgtt cccatcatat cgccacgtcc acgaacaaaa
    30541 ttagccatca tttgacccaa ttgtttgcga gatggattac gtacctggtg cccattccat
    30601 gggtaaattt ttccttcagg aataaatcca tctttgccgg ctttcaaccc ataggcagca
    30661 tattcccatt cggcttcagt tggaagtcga aaatcggaaa ccagtaaaat accgtccgac
    30721 atatccactt ttctgatttc gccggtcagg tctttttttg gatttttccc aaccggaggt
    30781 tgatagcttg cttgtttaag atatttttgc gtattaaaaa caaaattgtt aataatgtta
    30841 tcaaaattag tctcattttt taatgaggca aaatcgggtg gtgctattat acctgcttta
    30901 accaatgcca attcgtttac ccgatcggta cgccaactgc aataatccat tgcctgttcc
    30961 caagtaacgc ccacaatggg atattgatcg aaagcaggat gtgtaaaata atgttcaatg
    31021 taaggttcat tgtaagccaa ctcttcccgc catactttgc catttggata ggttttttcc
    31081 accaattttg gagctgtttt tccaaaaacg agattcatcc agtgagtgta ttctcgccaa
    31141 tcgacatttc tgatttcgta ttgatccatg tagaatgaac tcacggtaat tcgccttctt
    31201 cggctgtttc gaggagcagt caccacttct cccttttcgc ctattgtgaa tgagcctcct
    31261 tctatttcta ccatacctgg aggtacattg gttttaaaat cttttctgac agcaaatccg
    31321 gctccctttt catcattgta tttccaaccc gttacgcgtg agtatccttc tttgtttgat
    31381 tgacccgcac aacctaaaaa cagaagagct aaacagaatg tcgaaacttt aattaagact
    31441 tttactctca tggttttttt tgaataattg ttttttgaac tatgtttaac agaataaacg
    31501 atttataaat ttataaattt tgtcgtaata ttaatttgtt ttgaaaactt ctatgttctt
    31561 atctgaatct ataaaacaaa ttatttgcaa atatagtact ttttaaataa tactttcgtt
    31621 atgaagttcc aaaaaaatat ttttcctgaa gttatttgtc tcgattcctt tgttattaac
    31681 tgttttttta gaaaaaacgt ataattttca tttagttttt tcttttttta aagataaatc
    31741 aaaagttcgt cagctaaatt tcttattttt gcaatttgta gttgattccg aatccacaat
    31801 ttttaaaatt catgttttta aatttaaacg gccgtttggt tgatgtttct tctcctgttg
    31861 taatggggat tgtgaatgtt actcccgatt cattctatac gggtagtaga tttttgagcg
    31921 agaaatctat attaacttca gttgataata ttttaagtaa cggaggtact atcgtagatg
    31981 taggagcata ctcaagtcgt ccatctgctg agtcggtttc ggtagaagaa gaattgaaac
    32041 ggctgtcgag agcattagaa atcatattga aacaatttcc aaatgttatt ttatcggttg
    32101 atacttttcg atcaaaagtg gccagatatg taattgaaaa ttttcatatt gccatgatca
    32161 acgatatagg cggtggtaca cttgacgaat tgatgtttga aacagtagcc gacttgagag
    32221 ttgcttatgt tttaatgcac atgcggggta ctccgcaaac aatgcaaaca ttaacgcatt
    32281 atgaaaataa tgttgtttct gaagtttttt cgtttttaca gaaaggtctg gctcagcttc
    32341 atctattggg tgtgaaagat gtgatagttg atccgggttt tggttttgcc aaaactttgg
    32401 aacaaaatta tgaattgttg cataaactgc attatttaaa agagttgaac gccccgatat
    32461 tagttggttt gtcaagaaaa tcaatgattt acaatttact tcgactttca cccgaagagg
    32521 ctttaaccgg aacaattgca gccaatatgc tatctttgat aggcggagcc tctattttga
    32581 gagttcatga tgttaaagaa gctttacaaa ctatacggat atttgagcag tatcaaaact
    32641 tttcgacttt tataaatgat taaaaaaaga atttatgttt tttcagattg aatttaaaga
    32701 tattgtcgat atttttcttg ttgctttttt gctttatgaa atttacaaat tattgagaaa
    32761 ttcaggggct ataaatattt ttatcggcat attgacattt attgtttgtt ggttttttgt
    32821 gtcgtatgtt tttaaaatgc agttgcttgg tgaaattatg gatacggtag taaatgttgg
    32881 agcatttgct ttgattgtcc tttttcaaga tgaaatccga cgttttttta ctcaaattgg
    32941 cactaaaaga gaaaacagat ttttatcgaa aatgaaaaaa atttttggag aatcagaata
    33001 cgataaaaag gaaacagatt ttgacttgtt gcaggttgta ttggcttgtc gcaatatgtc
    33061 gagaacaaat accggatgtt tgattgttat tacaaggagg aatgctttgg aattttatgt
    33121 tcagtcgggt gaattgattg aagcaaaaat caattcaaga ttaatagaaa gcattttttt
    33181 taaaaacagt ccgcttcacg atggggcgtt gataatttcc cgacacacca taaaagctgc
    33241 cggctgcatt cttccggttt ctcaaaacca atcgatacca aaaaggttag ggttgagaca
    33301 tagagcggcg ctgggcatta ccgaacatac tgatgccgta gctattgtag tttccgaaga
    33361 aacaggtcat atatcttggg cagtcaatgg aaatttgact cacaacgtta aacccgaaga
    33421 gttggaatat tttttaaata cccaacttca gggaagcaac aaagtggagg aagaaaaaat
    33481 aaaataaacc gatacttttt ccttagattt ttttagaaaa agtatcggta aattatttaa
    33541 tcaaatatca tgttttcagg cctttcctgc acttcctagt acattcaccg ttttgtgcat
    33601 gtacaatttt ttcaattcat cgcgtgcagg tcccagatat tttcttgggt caaattctgc
    33661 cggcttggta gcaaaaattt cgcgaacagc tgctgtcatg gccagacgac catcagaatc
    33721 aatattgatt ttgcacactg cagaagtagc tgctctacgc aactgatctt cgggaacacc
    33781 aatggcgtct ttcaatgcgc caccatattt gttgattttt tccacatatt cgtgaggtac
    33841 ggaggatgca ccgtgtaaaa cgatggggaa acccggaata cgtttttcta tctcttccaa
    33901 tatatcaaag cgcaatggcg gtggaatcaa aattccgttt tcatcgcgtg tacactgttc
    33961 gggtttaaat ttggtagccc catgtgaggt ccctatggaa atggccagtg aatcgacatt
    34021 ggtgcgggtt acaaaatcaa ttacctgatc cgggtcggtg taggtatggt gctcggcaac
    34081 aacatcatct tcaataccgg ctaaaacacc caattctcct tcaacagtta catcaaactg
    34141 atgggcatat tccacaactt ttttagttaa agcaatattt tcttcgtaag ggagatgaga
    34201 accatctatc attaccgaag aaaaacctga ttcaatacag cttttgcaga gttcaaaagt
    34261 atctccgtga tccaaatgaa gtacaatggg aatattgtaa cccagctctt tggcatattc
    34321 aacagctcct tgtgccatat agcgcaataa tgtttggttg gcatatttgc gtgcaccgct
    34381 cgacacctgc aaaattaccg gcgattttgt ctctacacaa gcagaaatga tagcctgcag
    34441 ctgttccatg ttgttgaaat taaatgccgg gatagcgtat tttccttcaa ttgctttttt
    34501 aaataactct cttgaattga ctaatccaag ttcagtgtaa tgtaccatat tttttgaatt
    34561 ttaaataatt aaataatcaa actttgcaaa attacgaatt tttcctgaca aatgaaaaga
    34621 aaaatcttca ctttgttttt tcattccaat caattttcta tcaactaaaa cagaattttc
    34681 ttgccaattg tttgaaatag taaaataatt tttcttaaaa attttattaa tcaacctctt
    34741 aatttaattt tgcacattat caaacatttt aaatttaaag attatgaaac tttggaaaga
    34801 gtttaaagaa tttgccatgc gtggcagcgt aatagatttg gcaattggtg taattattgg
    34861 aagtgctttt ggaaaaattg tttcttctct tgttgctgat gttatcatgc caccaattgg
    34921 gttgctcatc agtggtatta attttacgga tttgaaattg gttttaaaac cggctgagat
    34981 ggtaaacgat gttcaaactc ctgccgtaac attaaattat gggaattttc tgcaagttgc
    35041 ttttgatttc ctgattgtgg cttttgtgat ttttcttttg ataaaaggta tcaacaattt
    35101 cttgaaaaag aaagaagaaa aacctgtccc tgaagcaaca gcacaaccga gtaaagaaga
    35161 aattttgctg accgaaattc gagatatttt aaaaaagaaa taaaaaacaa acaaaagaga
    35221 tgcttggttt atgttgcata acaaacctca gaacagagac aaaaacacaa tttccttttg
    35281 gtgcgattaa aagtttttcc gctgttgtga agttccgcat aaggaagtaa ggactgcaac
    35341 cagatggaat agatgtcaat cttttcggaa cgcaatgatt tgcgcaatgc gcctgtccgc
    35401 atcatgggca aaccaatgga attgtgtttg ttcgggttcc atgcttcggc aaagaaggtg
    35461 ttgcgttcga aattacgatc gaattcgtca ttcattttct cctgaatatc tttgagtaat
    35521 aattgaaaaa gttggttaat tgtttgcatt ttaaaaattt atattgtatg ttttcttttg
    35581 gttgagggga acatcacccc ccaaccccga ccttgggatt agctgtaaaa gttaatccca
    35641 ttgtattttt aacttaccgg acaatatttc ctccctgaga tattgttttt caaaaatatc
    35701 ttggtagatg acaaaaaatt gtgcaaacat ttatattatt tattttcaga tatttaatga
    35761 cggaaatttg ccacaaaccc cacacaagaa aaattggcgt tcgttggcta aaatctttta
    35821 aactcgctcc ttcgtcgctc aaacagaaaa gattttttta acgccaactt ctttttagct
    35881 acgctgaccg ttggttcggc tcaccggctc aggtcgcttg tttgcttcaa acaccgtttt
    35941 gtcggatttg caatatgaca ggatattgcc ggtggaggca taaaatcagc ggaaaaaatc
    36001 agcgtaaatc agcatgagaa aaacctctct cgccgatgat tgctgataaa tatcgcagat
    36061 aaacgcagaa aatcaataaa aatcaccgga agtctgcgtg agaaaataaa tgtgggttta
    36121 ttcaaacctt atttaccacc actttaataa cacaacaaat ccaccaccca aattgaattt
    36181 tatgagctta ttaaaaatta aaaaatccgg agattttatt tttacctgct acgcaggcag
    36241 gaagatatga caaatagaaa atgttcaact aaaaaatgtt tgtccttttc ttcgtctgtt
    36301 ttaaaaattt aacgttatat ttgttatctt tttagcaata cctcaatttc ataaaaacaa
    36361 ctgcaacatt aaataaaagc aataaacaca aaaattttat gactcatcaa aacggacttg
    36421 acgtaaaaac gctcgaaaac tggttgtggg aagccgcctg caaaattcgc ggacccattg
    36481 atgcccccaa atacaaagat tacattttac cgctcatttt cctaaaacga ctttccgacg
    36541 tattcgaaga cgaaatcaat cgtctttcgc aaaattacgg ttccagagaa atagtggagc
    36601 aatttctatc caacgaccat aatcttgtgc gtttttatct gccgcccgaa acccgttggg
    36661 aagccatagc taaacatact acaggcgtgg gcgagtattt gaccaatgcg gtgagaaaaa
    36721 ttgccagaga aaatcccaaa ttgcaaagcg tcatcgacat tgtggatttt aatgctacag
    36781 ccggcggaca aagaannnnn nnnnnnnnnn nnnnnnnnnc cttatcaacg tgctcggaaa
    36841 atatcgtttg ggattggacg atgtggaacc cgacattttg ggacgagctt acgaatattt
    36901 gctcagaaaa tttgccgaag gttcgggaca aagtgccggc gagttttata cacccagcga
    36961 agtagccgtt ttgatgtcgt atattctcga tccgaaaccc ggggacgaaa tttatgatcc
    37021 ctgctgcggt tcaggcggct tgctcatcaa agcttttttg cgaagcaagg aaaaattcga
    37081 cgataatccc tccttcgaac cggtacgatt ttacgggcag gaaattctgc atgctacgta
    37141 tgcgatggca aaaatgaatg cttttattca cgatatggaa gccgaaatag cactgggcga
    37201 caccatgaac cgtccggctt ttttaacgtc ggcaggtgcc ctgcggaaat tcgacctggt
    37261 tacggccaat cccatgtgga acc
//
LOCUS       DAOSIB010000012        36508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_1321_length_36508_cov_9.566538, whole genome shotgun sequence.
ACCESSION   DAOSIB010000012 DAOSIB010000000
VERSION     DAOSIB010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 36508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36508
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1321_length_36508_cov_9.566538"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..933)
                     /locus_tag="PLD44_02135"
     CDS             complement(10..933)
                     /locus_tag="PLD44_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOF98168.1"
                     /translation="MGFSGWSHAEKTIVVDINGTGDFRNIQQAIDSVRAFNPDGWVTI
                     YIKNGCYKEKIEIPTHICNIRLIGEDRDKTIISFDDHAKINNMGTFKTYTLKICGNDM
                     LVENLTVENAAAPVAQAVALHVEGDRIVFLNCRFLGNQDTVYAGRENSRQYFENCYIE
                     GTTDFIFGPATCWFEKCQIYCKRNSFITAASTPQNIPYGFIFNNCTVSLADSVKAVYL
                     GRPWRPYAMTVFMNCYLPKGIVPASWHNWKNPENEKTARYYECNNSGEGSRTDLRVKW
                     AKQLTKTECEKITIENVLGNFVEEMKKMKNE"
     gene            complement(983..2395)
                     /locus_tag="PLD44_02140"
     CDS             complement(983..2395)
                     /locus_tag="PLD44_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOF98169.1"
                     /translation="MKIFKELVFVCCLFTVGISASAQIPAFPGACGGGVFASGGRGGK
                     VLFVTSLADDNSEGTLRWAVNQKGARTILFKVSGIIPLTKTLTIKEPDLTIAGQSAPG
                     DGICIKNHDVFIAADNVIIRFIRFRLGNEILHNESDAVGGRNCKNVIIDHCSMSWSID
                     ECASFYTNEDFTLQWCIVSESLNNAGHAKGPHGYGGIWGGKNASFHHNLIAHNLSRNP
                     RFNGWRREGLKYNTSIDEERVDFRNNVIYDWGDNSSYGGENGKYNIVGNYFKYGPATK
                     PSVRFRITHVDMDSHPEMVSPGYGQYFIDKNFVYGCPECKKDNWECVSYAEGVNKQLC
                     KATHPFECTPIIEQSAEVAFKKVLQFAGASFRRDAVDKRIVKETQKGKATYKGSINGR
                     PGIIDSQNDVGGWPVYRSTEPYPDTNQDGIPDGWLEKNYPGKSATDMNEEGYTYLEVY
                     LNSLVEKITKSEYTNALSNK"
     gene            complement(2407..4050)
                     /locus_tag="PLD44_02145"
     CDS             complement(2407..4050)
                     /locus_tag="PLD44_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOF98170.1"
                     /translation="MFAQIPSFPGAEGDGMYTTGGRGGKILYVTKLTDDGTTGTLRWA
                     INQSGARIIMFKVAGIIPLTSELRIKNGNLTIAGQSAPGDGICLKNYPVVIQASNVII
                     RFIRFRMGDEAAAGNTSYSWDGADAIWGRNQSNIIIDHCSMSWSIDECSSFYDNKNFT
                     LQWCVIAESLRKSVHGKGTHGYAGIWGGAPATFHHNLLAFHDSRNPRFCGSRFSNSPS
                     TERVNFTNNVIYNWGSNSGYGGEGGYYNMMNNYYRSGIARSSGSTSYRIFSPNADDGT
                     NSQPKGVWGHFHLSGNIMINTPSVTDNNWLGFQPVGTYSDTIKSEVPFDFPPYLRLQR
                     ADSAYLSVTAFAGASLKRDAVDTRIIDNVLNGTVTVTQGSNGSTGGFIDSQADVGGWP
                     EYTYDPATVPTDTDGDGMPDDWETAKGLNPNDASDGSTKTIDGQYTNVEVYLNELAAP
                     VVNRQYLDANTAVNEVYANSSLRIYPNPFKSGNLNIKAAEKINKVEFYTLSGAKILEK
                     EMNGTEISFTMQQLNAGIYIVKVKMEDGIILPTLLIVEP"
     gene            complement(4118..7333)
                     /locus_tag="PLD44_02150"
     CDS             complement(4118..7333)
                     /locus_tag="PLD44_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOF98171.1"
                     /translation="MKKIFYFLLLFFFVGFSIIKAQTKIDVWDFGAAQFDPTIYNNML
                     DEATINSWYPGVTPGTTGKNLPSSFTVGILSWTSTSSTSDRLRTSNTNLTRYDSNGVP
                     VTVASTSETLTGYLYVNASAATGRYLSLNLNEDDEVTIYAKSQNGTGKMTFEYTSDNS
                     QKDIVSLTSSPEIYKFVAKKAGVYKIYDSVDKPFYYRILRKPATYVSVSGTFDLTNAS
                     DIPSGYTINFTNAAGKVWSVAPDASNNYSINLPAGYTYSLSLSNANGYIISNSDPLSV
                     SGNTTYNPVILKVTLYTLSGNITGLPSEKLAKLGLIFTPSDTRIYVPKPVVNASAGTY
                     SVQLEANCDYTISATGVNDYYISNNNVNISSDINTDIVFVAKPVYTVLLNINGLDATQ
                     LSKLSVTFSNLNESGYVYSFTDLNNIQLRDGVYSISCSGLDEYPVQLGLTSNLKVNGA
                     ETSKDLSFIPVTDWSFDDATITSGVTTAYKGMLFTGSVYNEKSKGHLVLTGNATAKVP
                     VSPGEKMIVYYYYSAKFTVDGGSEISTNSGSTNRIESHEFVYAESTPGYMTIANVTGT
                     TTYLTEIKMVKSVTYKPIITVGTDKEYQSINDALAAVRCMTRENTDTVKIMIDPGNYE
                     EMLVIDIPNVTLVNASTTPSIALLNKGVDIDENAVRITSYYGHGYNYFSMGTDQKWNA
                     DVLRVNKENGYTTYSNTGSGTTNGSYWNATVVVSASGFRADNIIFENSFNQYISRKES
                     EDVVQEWSTGGKGTRPTTYGSTDVQNKNFVERAAAIAFTASADRAILNKCRVVGRQDS
                     FYGAEGARIAVYKGVLMGATDYLFGGMTLTCYQTDLAMNTSEASSDVAYITAAQQNSA
                     RGFLMYECNITSAVPSVETASLYRSKPGYFGRPWQPTTSEVVFYSTTIETTNNPSFYG
                     QSLIVPTGWNNSLGGTSDKVYEYNTIEKSGVDNSASRVSWSKVLTEPVLSDGTEITTF
                     NFTKGTDNWDPINTLKSNDINASVSLQKKDDFIRIFSDGSKVFISNVKNPSKIKIYSV
                     NGSLSFVKEISGDDEISLDKGLWIVNVVNNESIKTAKVLIK"
     gene            complement(7372..8457)
                     /locus_tag="PLD44_02155"
     CDS             complement(7372..8457)
                     /locus_tag="PLD44_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOF98172.1"
                     /translation="MADSEMKRNPESWMLDFSKKLKWDYCNGLELQAFLKVWEKTGDE
                     KYFQYAKSYADTIIEENGQIKGYKLEEYNLDRINSGKFLFTLYEKTKDEKYKKAIELL
                     RSQINTQPRTSEGGFWHKKIYPHQMWLDGIYMASPFLAEYAYRFNEPALFDEVAFQIL
                     LIAKHCYDPATGLYYHGWDESRQQKWADPVKGTSPNFWSRSMGWYAMAIVDVLDFLPE
                     NHPKRPEIIKVLTDLCYSLDKYRDPKTGMWFQVTDKINEKGNYVESSGSAMFIYTWVK
                     GAQKGYLPKEFLKKGKKAYKQFVKTFIVENSDGTISLKNACAVAGLGGEKNYRDGSYE
                     YYISEPIRDNDPKAVAPFIMVSVLLNK"
     gene            complement(8583..9374)
                     /locus_tag="PLD44_02160"
     CDS             complement(8583..9374)
                     /locus_tag="PLD44_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HOF98173.1"
                     /translation="MKFRFSAITILGVLMLFFLASAPKPVKVLMAGDSTMADKVLYKT
                     VFDSLTGKSYEEPFLERGWGQLLPELLNDKALVKNYARNGRSTRSFIEEGLWTELLNN
                     TEKGDFVIIQFGHNDESVEKTGRYTNPAQFRLNFVAFVAEVRLKGATPVLCTPVARRN
                     FDNTGRLVPTYGSYPEIIRSVARDYRVPMIDLEKLTSEWLQNEGVEGSKKYFHKIAPG
                     ISRLYPNGLDDNTHFNEAGARTVAEMFVKEVATQKIKGFEKILKK"
     gene            complement(9371..10963)
                     /locus_tag="PLD44_02165"
     CDS             complement(9371..10963)
                     /locus_tag="PLD44_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HOF98174.1"
                     /translation="MLASAILITAHLNAKNPYEYLFKNLPFEMPKIQPPTFKNNVVRI
                     EDFGGKGDGKTLNTEAFKQAIDALARKGGGKLIVPAGVWFTGPIELKSNINLHLEKGA
                     LILFSKDFDLYPLVDTYFEGLATKRCQSPISGRNIENVAITGEGSINGSGEAWRPLKK
                     SKVTASEWKKVVNSGGVVKEGNYWFPSEKSYKGYSISDMNVPRAKLTDEEWMEIRDFL
                     RPVMVSFIQCKNVFLQGVLFENSPSWNIHPFMCENVIVDNIIVRNPDYAQNGDGIDVE
                     SCKNVIIVNSYFDVGDDAICIKSGKDEEGRRRAVPTENLIVHNCKVFKAHGGFVVGSE
                     MSGGVRNISVSCCQFLGTDVGLRFKSKRGRGGVVENIFVDGIYMFDILTDSFLFDLYY
                     GGKSATEAMEEGNDNQKDAVLMPVTEETPVFRNIFVKNIVSRNAHRAMFFNGLPEMKI
                     SNIQVENAIITATYGAELNNSKDIVFKNIYVEPTIGPALRLNRIENFKAENFTYPEKL
                     QNAIELKGEENENIIISNVKIK"
     gene            complement(11085..12692)
                     /locus_tag="PLD44_02170"
     CDS             complement(11085..12692)
                     /locus_tag="PLD44_02170"
                     /inference="COORDINATES: protein motif:HMM:NF027642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4957 domain-containing protein"
                     /protein_id="HOF98175.1"
                     /translation="MKKIFKNIPILFSLLIVFTVSSCKDDLPEEITELVTDRLFSPVG
                     VEAKVINKTQVVLNWTKNPKAESYVIEVYENDSLTFAGSPVMIFTNITNDDIPDTIKS
                     GLIGATQYSVRIKAVGSEIAESKWSAVYFKTGEEQIMNAVSEESVSAHSVKLTWKAGE
                     TATKIILTAGQSTLEHVVTAEEIAAGEATLSGLIEDTDYTARLMKDDKVRGSATFTTK
                     IDLNAANTIVVHPDEDLVVAIQGAASGSRIVLVASDDGSNEFLKSAGQTINIDKGISI
                     RGYLKSNKPTLHMQFRLTAENASLTVKDVILNGQSDADMRDHVVQLTQSVACGDILFK
                     DCIITNYGKSLISGASGIAVSVRSFTIQNCKVSNILTSSADCIDVRSGCIENATLLNS
                     TFYNCAPDRDFFRLDDASSSFPGKSTIIKVNRCTFSKVNNDAARRLLYVRFANNEITF
                     TNNVVANSVGYYTNQTATNIVLFNKNNYFNAPNYLVYTGTALNVKTDNSGTYLQQNPG
                     FVDEANGNLTITNATLLEQGIGANISW"
     gene            complement(12716..14503)
                     /locus_tag="PLD44_02175"
     CDS             complement(12716..14503)
                     /locus_tag="PLD44_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOF98176.1"
                     /translation="MKKTIFISAILTLFLMGIMSSCEETLEAPTKSSLDEQVIFSTPV
                     LAEGALMGIHQSLAETNSYRGRYIPFYGINTDIEWYNSSENVSDDRAKLTGYDPLPTN
                     TQMNMENNAWAKMYEAIERANLCIRGLRLYGNVEKNPELAQILGEALTLRAMIYNDLI
                     KAWGDVPARFEPISPSTLYLPKSDRDVIYKQIIADLAEAENYVAWPNETAVTRTTERV
                     NKAFVKGLRARICLAAAGYSQRPDGTIRRSNDPELSVENLYPIVKQECIDVIESKTCK
                     LGTFEQNFRNLCQDNVTAGLESLYEIPFSDGRGRVLYTFGVKHTTTDKYTQQAQGGQN
                     GPLPYVYYDFDKDDIRRDITCVPYEWTNGKQVPRKLSSWCFGKLRYEWMKRVVTSTND
                     DGINWQCMRYADIYLMAAEAINELDGPEAAAPYLREIRKRAFPNNPEKVDAYMASVTA
                     SKEAFFNAIVNERALEFCGESLRKADLIRWNLLSTKMNEAKAKLTRLANRQGEYADLP
                     EKIYYKTASDGESLIIYGLEHGHTDTEGAALNYESSKGWFVSNGVNTLTDDKINSLFQ
                     RDPDTRQFWPIWQVFIDSSNGLLKNDYGY"
     gene            complement(14525..17770)
                     /locus_tag="PLD44_02180"
     CDS             complement(14525..17770)
                     /locus_tag="PLD44_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOF98177.1"
                     /translation="MRTQKKFISILFFILVGFFVQVYGFTDQNNLIQQNERTIKGTVK
                     TTDGEPLIGVSVIVAGSPSAGTVTNLNGDFTITAPANATKLQFSYVGMKPLTVEIGNR
                     SVINVVMTEEAIGLNEVVAIGYGTVKRKDITGSVVSVSSDAIMNIPVSSPAEAISGKL
                     AGVQVTTTEGSPDAEIRIRVRGGGSITGSNKPLLIVDGFPVQSIDDISPYDIESIDIL
                     KDASSTAIYGSRGANGVVIITTKSGKEGKITANYNFYTAAKKIAKTLDVLSPSDYAKW
                     QYELALLKNDGDASSYEKYFGNYQDIDLYNNVPSNNWQDLVFGRTGTTFNHNLSINGG
                     DSKNKFSFSFSHIDDKAIMQMSSFKRDNINFKYTTNPSKNVSMNFSTRYINTKINGGG
                     MNEQKEVSSADSRLKYAMIYPPFPLKDLTSEAGTSDDTEIGNLYNPLVAISDNDRQQK
                     RNNLNMAGDITWKAFDNFKLKVEFGYENYRAEDQRFYGTTTYYIKNIPSSENQGYPAA
                     ELNNTFLNTFRNTNTVSYDFKKLLGTSHHLDVLLGEEYIFNSKQTLTNTIHGYPKSFT
                     STNAFRLTSQGTPYNIENYYSPDDILLSFFGRANYDYLSRYLMSVTFRADGSSKFSKG
                     NKWGYFPSAALAWRISSEPFMESTQSWLDDLKLRLSYGTAGNNNIPSGQMTQSFDSKS
                     TSWINNVASYWSPSKIMANPDLKWETTVTRNLGLDFVLLKSKLNGTIEGYYNTTSDLL
                     INFPVPGTGYDTQYRNMGETQNKGVEFTLNWIAIDKKNYGLSFSGNIGFNKNKINSLG
                     IMSDFGAASGWASTEIGNDYWIAAGGSVGQMYGYRSDGRYEVSDFEGYDSVKKKWILK
                     EGVVDCSSVIGSDALRPGAMKLKDITNDSIVNSKDYTIIGNANPLHTGGFTINGRLYG
                     FDLSANFNWSYGNDVYNANKIEYTSTSKYQYRNMLAIMADGKRWTNLKSDGTISNNPA
                     ELEAMNANTTMWSPYMSKYVFSDWAVEDGSFLRLNTLTLGYTLPVHLIRQIKIQQLRF
                     YVTGYNLYCWTNYSGFDPEVSTRRKTELTPSVDYSAYPKSRQVVFGLNLNF"
     assembly_gap    18590..18689
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18752..20002
                     /locus_tag="PLD44_02185"
     CDS             18752..20002
                     /locus_tag="PLD44_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HOF98178.1"
                     /translation="MSIKFRLILMNFLQYFIWGSWLISLGSYAGNILNFSGVQIGSIF
                     ASMGIVSLFMPGLLGIVADRWIPAHKMLGICHLLGSIFLIAASFQTQYMAFYSFMFIS
                     VMLYMPTISLSNTVAYNALEKNHYDIVKDFPPIRVWGTIGFICAMWTVDFTGFKFSQA
                     QLYVAATAALILGVYSFTLPPCTVNRSNEKKSVIDVLGLRAFTLFKHKKMAIFFIFSM
                     LLGASLQITNGFGDLFLRSFSEIPEYMNSFGVRHSVVLLSISQISETLFILTIPFFLK
                     KFGIKNVMLMSMFAWVFRFALFGMGNPGDRLWMLILSMIVYGMAFDFFNISGSLFVEQ
                     STDSSIRASAQGLFMIMTNGLGATFGSYLAGAIVDKFVTHAEVPNWSTAWYIFAGYAL
                     IVGILFAILFKYKHNTQEVTEKFH"
     gene            complement(20171..22762)
                     /gene="clpB"
                     /locus_tag="PLD44_02190"
     CDS             complement(20171..22762)
                     /gene="clpB"
                     /locus_tag="PLD44_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOF98179.1"
                     /translation="MNFNNFTIKSQEAIQEAVDIVQSKGQQAVETPHLLKGVLLKGED
                     VIQYLFGKMGVNLSMLNSVIDRMIETYPRVSGGEPYLGRETNAVLQKAIDISSKAGDE
                     FVALESILMALVVENNSVKKALNDAGVNEKSLSEAINELRKGSKVNSASAEDTYNSLN
                     KYAINLNERARSGKLDPVIGRDEEIRRVLQILSRRTKNNPILIGEPGTGKTAIVEGLA
                     HRIVRGDVPENLKSKQIYSLDMGAVIAGAKYKGEFEERLKSVVNEVVNSNGEIILFID
                     EIHTLVGAGKSEGAMDAANILKPALARGDLRAIGATTLAEYQKYFESDKALERRFQIV
                     MVDEPDIPSSISILRGLKERYENHHKVRIKDDAIIAAVELSSRYITERFLPDKAIDLM
                     DEAAAKLRLEIDSVPEELDVIERNIKQLEIEREAIKRENDTKKLEQLSQEIANLKEQA
                     DALRAKWASEKSLIDKIQQAKIDIENLKFEAEKAEREGNYGKVAEIRYGKIKALESTI
                     AQYQEELAKIQGNNPLIKEEVDSEDIAEIVSRWTGIPVTKMLQSEKEKLLHLEEELHK
                     RVVGQDDAIQAVADAIRRSRAGLQDPKRPIGSFIFLGTTGVGKTELAKALAEYLFNDE
                     NMMTRIDMSEYQEKFSVTRLIGSPPGYVGYDEGGQLTEAIRRKPYSVVLFDEIEKAHP
                     DVFNVLLQVLDDGRLTDNKGRTVNFKNTIIIMTSNIGSSLIRENFEKINEYNHDEIIE
                     RTKNEVFELLKQTIRPEFLNRIDELIMFKPLDRSQIEDIVRLQLDGVRRMLEQNGIRL
                     ETTDRAVKFIADNGYDPQFGARPVKRAIQKFILNDLSKKIIAGEIVSNQVVVVDADEN
                     GIVFKNK"
     gene            23055..25778
                     /gene="ppdK"
                     /locus_tag="PLD44_02195"
     CDS             23055..25778
                     /gene="ppdK"
                     /locus_tag="PLD44_02195"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOF98180.1"
                     /translation="MDNKKRVYTFGNGQAEGSASMRNLLGGKGANLAEMNLLGIPVPP
                     GFTITTEVCTEYYALGKDKVVELLKPEVEKAMEGVEKLMRSKFGDKKNPLLVSVRSGA
                     RASMPGMMDTILNLGLNDEIVEGLAEKTGNPRFAWDSYRRFIQMYGDVVLGLKPANKN
                     DIDPFEAVIEQLKEERGVKLDTELTVDDLKELVKRFKALVAERTGKEFPLSVYDQLWG
                     AICAVFDSWMNERAIFYRKMEGIPSEWGTAVTVQAMVFGNMGNTSATGVCFTRDAATG
                     EDVFNGEYLINAQGEDVVAGIRTPLQITKQGSQQWAKLTGISEEERKEKYPSMEEALP
                     EIYKELNEVQEKLEKHYKDMQDMEFTVQEGKLWLLQTRNAKRTGTAMVKIAIDMLHQG
                     IIDEQTALLRVDPLRLDELLHPIFDKNAVKKAKVIAKGLAASPGAASGKIVFNADDAA
                     EWAARGEKVIMVRIETSPEDLAGMAAAQGILTARGGMTSHAAVVARGMGKCCVSGAGS
                     LKIDYAKKTMEADGIVLREGDYISLNGSTGDVYLGQIPTQEAELDADFAELMTLADKY
                     SSMKVRTNADTPHDAAVARKFGAVGIGLCRTEHMFFEGEKIKAMREMILAENEEGRRK
                     ALGKILPYQQADFEGIFEVMDGFPVTVRLLDPPLHEFVPHDEKGQKEMAEAMGLPLQK
                     IQQRVEELREQNPMLGHRGCRLGNTYPEITEMQTRAILGAALKLKSKGIKIIPEIMVP
                     LVGIKKEFLEQERIIRETAEKLFAEKGDRIEYKVGTMIEVPRATLIADQIAENAEFFS
                     FGTNDLTQMTFGFSRDDIASFVPDYIEKKIIEKDPFQVLDQEGVGQLIKLGVEKGRST
                     RSDLKIGICGEHGGEPSSIEFCYRVGMNYVSCSPFRVPIARLAAAQAALKN"
     gene            26321..26677
                     /gene="rsfS"
                     /locus_tag="PLD44_02200"
     CDS             26321..26677
                     /gene="rsfS"
                     /locus_tag="PLD44_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOF98181.1"
                     /translation="MEQSEQIVNKIVEGIQEKKGKKVVIINLTGLKNAPCSYFVICEG
                     TTSIQVDAIGTSIKDTVREKMNIKPYAIDGLRNSEWVAMDYGSVIVHIFRPQIRNFYD
                     IEHLWEDAEITEIYDE"
     gene            26735..28738
                     /gene="ftsH"
                     /locus_tag="PLD44_02205"
     CDS             26735..28738
                     /gene="ftsH"
                     /locus_tag="PLD44_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOF98182.1"
                     /translation="METKSPQNSPQKPGKGPKFNITWLYVIIIGILIGSYLFSDTAPV
                     KEVPFSDFEKYMYNNKMEKIDVYSSKNIAEALIIKDSLKQVFGEKYEMYEKEPKIRTR
                     IPSAEEFSKIYQKAKEQYGLTAVVDYKESRNYVDIFLYSILPFLLLILFFVYMNRRMA
                     NQMGGGSGGGIFNVGKSKAQLFDKGDVANRITFKDVAGLEEAKQEVEEIVEFLKNPSK
                     YTELGGKIPKGVLLVGPPGTGKTLLAKAVAGEADVPFFSISGSEFVEMFVGVGASRVR
                     DLFRQAKEKAPCIIFIDEIDAIGRARGKNPNFGSNDERENTLNQLLTEMDGFASNSGV
                     ILLAATNRADILDKALLRPGRFDRQIHVDLPDVHGRKEIFKVHLRPIKIDKSVDIDFL
                     AKQTPGFSGADIANVCNEAALIAARKNKSFVEKQDFLDAVDRIIGGLEKRTKITTKFE
                     KKSIAYHEAGHATISWMLEHASPLVKVTIVPRGEALGAAWYLPEERQLTVRAHMLDEM
                     CSTLGGRAAEELFLNQTSTGAINDLERVTKRAYAMVTYFGMSEKLPNLSYYDSSGSAE
                     YSFTKPYSEKTAELIDEEVKNIVSREYERAKEILREHAEGHNRLAELLIEREVIFSED
                     LERIFGKRPWASRSDELLAQNGENGKSSERMEDNTVENENKEE"
     gene            28719..29384
                     /locus_tag="PLD44_02210"
     CDS             28719..29384
                     /locus_tag="PLD44_02210"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HOF98183.1"
                     /translation="MKIKKNDLINEEELSSREIILKKISDILCTRTSETIFSEYFSHQ
                     IYREIAPDMLTCFMEEIKAVSGECFVYDSPSNLFVALKKFLLGKGFSKIHCRNEKITE
                     YLKSFDIPFSSSEADFEIMEVGITDCEFLVARTGSVIVSSFNSGRKMNIFPPVHIVIA
                     HRSQLVSYLDDAYRQILQKYKNNLPSLISTITGPSRTSDIEKTLVLGAHGPKQLLVFL
                     LDN"
     gene            29449..30261
                     /locus_tag="PLD44_02215"
     CDS             29449..30261
                     /locus_tag="PLD44_02215"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOF98184.1"
                     /translation="MNEMLKRAISGFIYVGLIVTSILVHPLAFAFVFGILTYCALHEF
                     YKITYRPSDIEVPISLASMGGLLLFVSTFLFKSGFSSPLIFSYYAIFVIWMFIRELFL
                     KKSNPIHNWAYFILGQVMVALPMSLLIFILFVDGYQPMLLLSIFILTWLNDTGAYLIG
                     VKFGRHRFFEHISPKKTWEGFFGGIFFSLTGGFILSLFIAELSIFQWLALCFITVLAA
                     TLGDLTESLLKRTLNIKDSGNLLPGHGGLLDRLDSMLLVSPFVLIFFLYIFV"
     gene            30350..31519
                     /locus_tag="PLD44_02220"
     CDS             30350..31519
                     /locus_tag="PLD44_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOF98185.1"
                     /translation="MEMNFDDIRCYTNEEVHEVLEKLTKEKSFMKVVSTAYPLLPKEI
                     IKERLLQYNNQQEFQKDFIFPFLQYLEENTTKGIELFGLEKINVSKAYLYLSNHRDIV
                     MDSSFFCKKLLENSIDTVEIAIGDNLLIYPWIEDFVKLNKSFIVKRGLNTRQTLESSK
                     LLSAYLLHTICEKNQSIWMAQREGRAKDSDDYTQESVLKMLNLSAKDNNFIQHILQFN
                     ICPLSISYEYDPCDYLKAKEFQLKRDFPDYKKSSTDDLENMVIGIMGYKGKVVFRITG
                     CINDELKKIEQKTSNRHEQISQTAQLIDLKIHANYEIYSVNKIAFDTLTGENRFAHEY
                     SNMERIDFEQYLMKQIDKIQLENKDEKFLRQKLLEMYANPLINHLKTLQHSQPSV"
     gene            31772..32671
                     /locus_tag="PLD44_02225"
     CDS             31772..32671
                     /locus_tag="PLD44_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007483621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98186.1"
                     /translation="MRKLLLLLVLGAQTIFAQNIQVTAVSPINTGESGGMYHPVFSPT
                     GEYLLFTSENYEGLKMLSFTDGQVKTLTTEKGAGYGVKISKDGKTIAYRKNELINQRL
                     FSSVKLYSLSEGKQQQLVAPTREPLAVGFSGNKPLLVKNRQLVKSQLSAVEIVPIVTI
                     EDRKIVLYTGNSRKILTPNGENASYIWPSISPDGKRIVYVAAGKGTFVCDLNGNNVAA
                     LGKLNAPQWLNNQWLVGMNDIDNGEILLSSSLVAASIDGKVYQTLTTPDLIAMYPAVS
                     PDGKQIVFNTERGDLYLLNVVLK"
     gene            32687..36358
                     /locus_tag="PLD44_02230"
     CDS             32687..36358
                     /locus_tag="PLD44_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOF98187.1"
                     /translation="MKKIISFFFVLMLIFSAKAVDFTGIKIYINPGHGGYNGANDRNV
                     QTIPFALGDTLGFWESASNLTKGLALRDLLQAANATVYMSRTQNREEDDRALSEIAEE
                     ANANNVDAFLSIHSNALGTNTGTNYLLLLYHGYDDQPTVAASLPMAQTAWPRLISNQL
                     TVWTHYTTSTNIRGDFSFYGNTSGLGVLRPLTVPGFLSEGSFHDYLPETHRLLNKDYR
                     KLESNNFYRYFCDYFQTNLPSTGIIAGFAKSSNEKMNNPLYIYKAFTHDEKVPINGAK
                     VKLMNQNGDSLNVYYVDTLYNGIFAFYNLAPGTYKLHLEAKDYQPKDTTVTVTAASTT
                     YAKMFLPNLNVVIPKDTTPDYPDPIQEAGVLPMKHYNFGDQEVVVPEWLNQQPIKKVL
                     YRDEKLYVLTTEPKITIYNAQSLAKIREMDLSGIESGVNLVSDIAFTSDGYLLACNKD
                     TISLPENKGRYFKVYTWDNDSVAPSLLFKTQQQANWSNGIMGETFAVSGPRWKCTIYV
                     PSVTTGSSKAIRIVGYLYEEGNALGYKYMMDAANYTEALWGENMKFTISPSGKDHFIL
                     DSEKVLPVEYQFDWNAADRSPLIKKGEFSEQNGFNLAVCASGIHFFRHAQHTFMVSPT
                     CDADTTKVGAVLFDVTNGIQQSLKVSEKLPANGLGTAKASYMVAAAKVSGYDLDVMVL
                     AQNEGMARFRTVAGPAVANIYASELKVEKTETDFMLKFTLNENATSVKIDILDGENII
                     KTIDAGALNKGVQSVPVPLTELPSGSFTWSVTASTEAVDRPVKISDDSRPELQFYSPR
                     GVAVDNNFESPFFGRVYATETVGGNTTNRTTQDGVYILNAALEDVTQQGAQSYAGSVS
                     WAGSSSPMRLAVAEDGKVYVTDWSDAHPGVWIMDPAAPSAPFVPVFSGLTKATSGLSS
                     INGVNVHGSISHCWVLGKGEDTRLYTFDEDYVDAVATSAGNVLQYNIGTLATSWQQAP
                     SAIVYNDALNGNLQQNYNSCIAPDGRGGWWISQYRSADAANIPSLIHVNTDGLVDFNS
                     GKTPTLIENSYTGGMAVTVDGKTLAMGCNNEVKIFSVTFSEDGIPSLTRLYSIKPAMG
                     TNTAGLAFDRAGNVYVISNSTERLGVWSLPKIENVFTTPAPASEKLDVISEVPQLEKE
                     KTEILLYPNPATDHVVISSKTAHLKTIAVYDTKGRLIRAMNAAGNQMNLSLEGMAPGL
                     YILQVQTDERLNSFRLIKR"
BASE COUNT        11095 a         7154 c         6679 g        11480 t
ORIGIN      
        1 aagaagattt tattcgttct tcattttttt catttcttcc acaaaattac cgagtacatt
       61 ctctattgtt attttttcac attccgtttt tgtaagttgt tttgcccatt ttacgcgtaa
      121 gtcagttctg cttccttcgc cggaattgtt gcattcataa taccgggctg ttttttcatt
      181 ttccggattt ttccagttgt gccaactggc agggacaatt cctttgggta aataacaatt
      241 cataaaaaca gtcatggcat aaggtcgcca cggacgtccc agataaacag ccttaaccga
      301 atcggcaagt gaaacggtac aattgttaaa aataaatccg taagggatat tctgtggtgt
      361 actggctgct gtaatgaagg aatttcgttt gcagtaaatc tgacatttct caaaccaaca
      421 agtagccggt ccaaaaataa aatcggttgt tccttcaata taacagtttt caaaatactg
      481 acgtgaattt tcgcgtccgg cataaacagt atcctgattt cccaaaaaac ggcaattcag
      541 aaaaacgatt ctgtctcctt ccacatgcaa agctaccgct tgagccacag gtgcagcagc
      601 attttcaacc gtcaaatttt caaccagcat atcgttacca caaattttta acgtataggt
      661 tttaaatgtt cccatgttgt tgatttttgc atggtcgtca aaactgataa tagttttgtc
      721 tctgtcttcc ccaatcaagc ggatgttgca aatatgagtc ggtatttcga ttttctcctt
      781 gtaacatccg ttttttatgt aaatggtaac ccagccatcc ggattaaaag cccgaacaga
      841 atctatggct tgctgaatat ttctaaaatc gcctgtgccg tttatatcca ccacaatagt
      901 cttctcggca tgggaccatc cagaaaaacc catgaaaaaa atcaacaata ttggaaaaaa
      961 ctttttcatt tttatccaca atttatttat tggacaaagc attggtatat tccgatttgg
     1021 tgattttttc aaccaaactg ttcaaataaa cttccaaata agtataacct tcttcgttca
     1081 tatcggtagc agatttgccg ggataatttt tttcgagcca tccatcagga atcccatctt
     1141 gattggtgtc aggatagggc tctgtagaac gataaacagg ccagccaccc acatcatttt
     1201 gagaatcgat aataccgggt ctgccattaa tcgaaccttt ataagttgct tttcctttct
     1261 gcgtttcttt cacaattcgt ttatccacag catctcttct gaatgaagct cctgcaaatt
     1321 gcaatacttt tttgaaagca acctccgcag attgttcaat aataggcgta cattcaaaag
     1381 gatgtgttgc cttacaaagc tgtttgttaa caccttcggc ataagacaca cattcccagt
     1441 tgtctttttt acattctggg caaccataca caaaattctt atcaataaaa tattgtccat
     1501 agccgggaga aaccatttca gggtgactat ccatatccac atgcgtaatt ctgaatctga
     1561 ccgatggttt ggtggcaggt ccatatttga aataatttcc aacaatgttg tattttccgt
     1621 tttcgccgcc ataagaacta ttgtcgcccc agtcgtaaat cacattattt ctgaaatcga
     1681 cgcgttcttc atcaatggaa gtattgtatt tcaatccttc ccgtctccaa ccgttaaatc
     1741 gtggattgcg acttaagtta tgcgcaatca gattgtgatg aaaactggca ttttttccac
     1801 cccaaattcc gccgtatcca tgtggacctt ttgcatgacc tgcattattc aaggattctg
     1861 aaacaatgca ccattgcaag gtgaaatctt cgtttgtata aaaagaagca cactcatcaa
     1921 tgctccaact catggaacaa tggtctatta tcacattttt acaatttctt ccgccaacag
     1981 catcgctttc attgtgcaat atttcattcc ctaatctaaa gcgaataaaa cgaataataa
     2041 cgttatcagc cgctataaaa acatcatgat ttttaataca aattccgtcg ccgggtgcac
     2101 tttgcccggc tattgtcaaa tcaggttctt tgatggttaa tgtttttgtc aacggaatga
     2161 ttcccgaaac cttaaaaaga atggttcttg cacctttttg attgacagcc caacgcagcg
     2221 taccttccga attatcgtcg gcaagcgaag ttacaaaaag cacttttcct ccccgtccac
     2281 cggaagcaaa tactccgcct ccacaagctc cgggaaaggc cggtatctga gcggaagcag
     2341 aaataccaac cgtaaaaaga caacacacaa aaaccaattc tttaaagatt ttcattttga
     2401 aaaatattat ggttcaacta tcaaaagtgt tggaagaatt attccgtctt ccattttaac
     2461 ttttacaata taaattccgg catttagctg ctgcatggtg aatgaaattt ctgttccatt
     2521 catttccttt tctaaaattt ttgcaccgct caaggtatag aattcaactt tattgatttt
     2581 ttcagcagct ttgatattca aatttcccga tttgaaagga ttgggataaa tgcgcaaaga
     2641 agaatttgca tatacttcat ttacagcagt atttgcatcc aaatattgtc gatttacgac
     2701 tggtgcagcc aattcgttca aataaacttc aacattagtg tattgtccgt caatcgtttt
     2761 tgtacttcca tctgaagcat catttggatt caagcccttt gccgtttccc aatcgtcagg
     2821 catgccatcg ccatcggtat ccgttggaac agttgccgga tcataagtat attcgggcca
     2881 accaccaaca tctgcctgag aatcaataaa accaccggtg cttccattgc ttccttgtgt
     2941 aaccgtaaca gttccattca gcacattatc aattattcgt gtatccactg catcacgctt
     3001 taaactggca ccggcaaaag ccgttacgct caaataagca ctgtcggcac gttgcaatct
     3061 caaataaggc ggaaaatcaa aaggaacctc tgatttaatc gtatcggaat aagttccaac
     3121 aggttgaaat cccaaccagt tattatcggt aacactgggt gtattgatca taatgtttcc
     3181 gcttaaatgg aaatgtcccc aaacgccttt tggttggcta ttcgttccgt catcagcatt
     3241 tggactgaaa attctataac ttgtagaacc gctacttcgg gctatgccgg agcggtaata
     3301 attattcatc atgttataat agcctccttc acctccatag ccggaattgc ttccccaatt
     3361 gtaaatgaca ttatttgtaa aattaactct ttcagttgat ggactatttg aaaatctgct
     3421 tccacaaaaa cgtggattcc tgctatcgtg aaaagccaat aaattatgat gaaaagtagc
     3481 aggcgccccg ccccaaattc cagcataacc atgtgtacct tttccgtgaa cagattttct
     3541 cagactttca gctattacac accattgaag tgtgaagttc ttgttatcgt aaaaagatga
     3601 acattcatca atactccaac tcatggaaca atgatcaata atgatattgc tttgattacg
     3661 cccccaaatg gcatcggctc catcccaact gtaagaagtg tttcctgcgg ccgcttcatc
     3721 gcccattcga aaacgtataa aacgaataat cacgttactt gcctgtatta ccaccggata
     3781 atttttcaga caaattccat cacctggagc actttggccg gcaatggtca gattcccgtt
     3841 ttttatcctg agttcggaag taagcggaat aattccggcg actttgaaca taatgattct
     3901 tgcacccgac tgattaatag cccagcgcaa tgtgcccgtt gtcccgtcat cggtgagttt
     3961 ggttacataa agaatttttc ctccgcgtcc gccggtagtg tacatgccgt cgccttcagc
     4021 acccggaaaa gacgggattt gggcaaacat ctgtaacaaa aaaatgtttg aaactaaaac
     4081 acacaaaatt gttaaaattc tatttttcct catcatttta ttttattaaa acttttgctg
     4141 ttttaatgct ttcgttattc accacattta caatccacaa tcctttatcc aatgaaattt
     4201 catcatctcc ggatatttcc ttaacaaaag ataaagagcc atttacggaa taaattttta
     4261 ttttggaagg attttttaca tttgaaataa agactttgct cccatctgaa aatatccgaa
     4321 taaaatcatc tttcttttgc agtgaaacac ttgcattaat atcattagat tttaaagtgt
     4381 ttattggatc ccaattatca gttccttttg taaaattgaa agtagtaatt tctgttccgt
     4441 cgcttaaaac cggttctgta agcacttttg accaagaaac gcgagaagca gaattatcaa
     4501 ctccagattt ttctatggtg ttgtactcgt aaaccttatc ggaagttcca cccaaagaat
     4561 tattccatcc cgtagggaca atcaaggatt gcccgtaaaa actcggatta tttgtagttt
     4621 caatggttgt agagtaaaat accacttcgc tggtagttgg ttgccacgga cgaccaaagt
     4681 atccgggttt ggagcgataa agtgaagctg tctccacaga aggaaccgcc gaagttatat
     4741 tgcattcata cattaaaaat ccgcgagcag aattttgttg tgcagctgta atgtaagcaa
     4801 catccgagct tgcttcactt gtattcattg ctaaatcggt ttggtagcaa gtcaatgtca
     4861 tccctccaaa caagtaatcg gtggcaccca taagtacgcc cttataaaca gcaattctgg
     4921 cgccttcagc tccataaaat gaatcctgac gaccaacaac tcgacatttg tttaaaattg
     4981 ccctatcggc acttgccgta aatgcaatag ctgcagctct ttctacaaag ttcttattct
     5041 gtacatccgt actcccgtaa gttgtaggac gggtaccctt tcctccggtt gaccattcct
     5101 gaactacatc ttccgattct tttcttgaaa tatattgatt aaaagaattt tcaaaaatta
     5161 tattgtcagc tctgaaaccg gaagctgaga ctaccactgt tgcattccag taagaaccgt
     5221 tagttgtgcc acttccggta tttgaataag ttgtataacc attttcctta ttgactctca
     5281 gaacatctgc attccatttt tggtcggtgc ccatgctgaa ataattgtat ccgtgtccat
     5341 aatacgaagt aattctcaca gcattttcat caatatccac acctttgttt aaaagagcaa
     5401 tacttggtgt tgtagaagca ttaaccaaag taacgtttgg aatatcaatc accaacattt
     5461 cttcgtaatt tccgggatct atcattattt ttacagtatc tgtattttca cgcgtcatac
     5521 aacgaacggc agctaatgcg tcattgatac tctgatattc cttatcggtt ccaaccgtta
     5581 ttattggctt gtaggttaca gattttacca ttttaatttc agttaaatag gtggtcgttc
     5641 ctgtaacatt agcaatggtc atgtaaccgg gagtagattc cgcataaaca aattcgtgcg
     5701 attcaatgcg gttggtactt ccgctgttgg tagaaatttc acttcctcca tccacagtga
     5761 acttggcaga ataatagtaa taaactatca ttttttcacc gggtgaaaca ggaactttag
     5821 ccgttgcatt acctgtgagt accaaatgtc ccttcgattt ttcattgtaa accgaaccgg
     5881 taaacaacat tcctttataa gccgttgtaa caccacttgt aatggtggca tcatcaaaag
     5941 accaatccgt aacgggaata aacgataaat cttttgaagt ttcagctcca ttaactttta
     6001 agtttgacgt aagtcccaat tgtacaggat attcgtccaa accactgcac gaaatggaat
     6061 agactccatc acgcaactga atattattca aatcggtgaa agaataaaca taacccgatt
     6121 cattcaggtt ggagaaagta acagaaagtt tggaaagttg cgtagcgtct aaaccgttaa
     6181 tatttaacaa aacagtataa accggcttag ctacaaatac aatatcggta ttaatatctg
     6241 aagagatatt tacattattg ttggagatat aataatcatt tacacctgta gcagaaatcg
     6301 tataatcgca atttgcttcc aactgaacag aataagtccc ggcagaagca ttcactacag
     6361 gtttggggac ataaattctg gtatcagaag gagtaaaaat taatcctaat ttcgccagtt
     6421 tttcggaagg caatccagta atattcccac ttaatgtgta aagtgtaact tttaagataa
     6481 caggattata agttgtattt ccactcaccg acaaagggtc ggaattggat ataatatagc
     6541 cgttggcatt actgagactt aaagaataag tgtatcctgc aggcagattt atggaatagt
     6601 tgttagaagc atcaggagct actgaccaaa cctttccggc agcattggta aaattaatcg
     6661 tgtatcctga aggtatatcc gaagcgttgg tcaaatcaaa cgtaccactg acggaaacat
     6721 acgtggcagg ttttctcaaa attctgtaat aaaatggttt atcaacggaa tcataaattt
     6781 tatatacccc cgctttcttt gctacaaatt tatatatttc cggtgatgaa gttaaagaaa
     6841 cgatgtcttt ctgagaatta tccgacgtat attcaaaagt cattttcccg gttccatttt
     6901 gagatttggc ataaatggta acctcatcgt cttcgtttaa atttaagctt aaatatcttc
     6961 cggtagcagc gctggcatta acataaagat atccggttaa agtttctgaa gttgaagcta
     7021 ccgttaccgg aacaccattt gaatcgtaac gcgtaagatt ggtattggat gttctcaagc
     7081 ggtcgcttgt agaagaagta ctggtccagc ttaaaatacc gacggtaaat gagctgggta
     7141 aatttttccc tgtggttccg ggtgttacgc ccggatacca actattaatt gttgcttcat
     7201 ccaacatgtt gttgtaaata gtaggatcga attgtgccgc accaaaatcc caaacatcta
     7261 ttttggtttg agcctttata attgaaaaac ctacaaagaa gaataatagc agaaagtaaa
     7321 atattttttt cattggtatt tttttaaaat ttattgataa ggtatgtctc tttatttatt
     7381 cagcaataca ctcaccatta taaaaggggc aaccgctttc gggtcattgt ccctgatggg
     7441 ttcggaaatg taatattcat aactgccgtc gcgataattt ttttcgccac ccaaaccggc
     7501 tacagcacag gcatttttca atgaaatagt tccatcagaa ttttcgacaa taaaagtttt
     7561 tacaaattgc ttgtaggctt ttttaccttt cttgagaaat tctttgggca aataaccttt
     7621 ttgcgctcct tttacccaag tataaataaa catggcagaa ccgctcgatt cgacataatt
     7681 tcccttttca ttgattttat ccgtaacctg aaaccacatt cctgttttcg gatcacgata
     7741 tttgtcgagc gagtagcaca aatcagtcaa aactttaatt atttcgggtc gttttggatg
     7801 attttcaggt aagaaatcca gcacatccac tattgccatt gcataccagc ccatgcttct
     7861 gctccagaaa ttgggtgaag ttcctttcac agggtcagcc catttttgtt ggcgactctc
     7921 atcccaaccg tgataataca gcccggtggc aggatcataa caatgtttgg caataagcag
     7981 aatttgaaaa gctacttcgt caaataatgc cggttcatta aaccggtaag catattcagc
     8041 caaaaaaggt gaagccatat aaataccatc cagccacatt tgatgcggat aaatcttttt
     8101 gtgccaaaaa ccgccttcgc tggtgcgagg ctgcgtattt atctggctac gaagtagttc
     8161 aattgctttt ttgtattttt catctttagt tttttcatat agagtgaaaa ggaatttgcc
     8221 agagttaatt cgatccaaat tgtattcttc aagtttgtac ccttttattt gtccattttc
     8281 ctcaataatg gtatcggcat aacttttggc atactgaaaa tatttttcat cgccggtctt
     8341 ttcccagact tttagaaaag cctgcaattc aagcccgttg caataatccc atttcagttt
     8401 ttttgaaaaa tcaagcatcc agctttcagg attacgtttc atttccgaat cagccattcg
     8461 aacgtaatat tttgattctt ttggagttaa actgaagcac gttaatgaaa atattaccag
     8521 caaataaagt tttctcattg ataatcggtc ttttaaatta aaaataaact tattttaaaa
     8581 attcattttt tcaaaatttt ctcaaatcct ttgatttttt gagtcgcaac ttcttttaca
     8641 aacatttcgg ctaccgttcg agctccggct tcattgaaat gagtattgtc atccaatcca
     8701 ttgggataca ggcgactaat acccggcgct attttatgga aatatttttt tgaaccttct
     8761 acaccttcgt tttgcaacca ttcagaagtg agtttttcca aatcgatcat tggaacccga
     8821 taatctcgag caacactgcg tataatttcg gggtaactgc cgtatgttgg tacaaggcga
     8881 cctgtgttgt cgaaatttct tcgggctacc ggagtacaaa gaacaggagt cgcacctttc
     8941 aatcttactt cggcaacaaa tgccacaaaa ttcagcctga attgcgccgg attggtataa
     9001 cgacccgttt tttctacaga ctcgtcattg tgtccaaatt gaataatcac aaaatcacct
     9061 ttttcggtat tgttcaataa ctctgtccac aatccttctt ctataaacga tctggtactt
     9121 ctgccatttc tggcataatt tttcaccaaa gccttatcgt tgagcaattc gggcaataat
     9181 tgtccccatc cccgctcaag aaaaggttct tcataagatt ttcccgtcaa actgtcaaaa
     9241 actgttttat acagcacctt atcggccatt gtagaatcac ctgccatcaa aaccttgaca
     9301 ggtttaggtg ctgaagcaag aaaaaacaac atcaaaacac caagaatcgt aattgctgag
     9361 aacctgaact tcatttgatt ttaacatttg aaataattat attttcgttt tcctctcctt
     9421 tcagttcaat tgcattttga agtttttcgg gataggtgaa attttcggct ttaaaatttt
     9481 ctatccgatt taaacgcaga gcaggaccga ttgtcggttc aacataaatg tttttgaata
     9541 ctatatcttt ggaattattc aattctgctc cataagtagc tgtgataatt gcattttcaa
     9601 cctgaatgtt gctgattttc atttcaggca acccgttgaa aaacattgct ctgtgtgcgt
     9661 tgcgcgaaac tatgtttttt acgaaaatat ttctgaaaac gggtgtctct tctgtaacag
     9721 gcatcaatac agcatctttc tgattatcgt ttccttcttc catagcttct gtagcggact
     9781 tgccaccata atacaaatca aataaaaatg agtcggttaa aatatcgaac atgtaaattc
     9841 cgtcaacaaa aatattctca acaacgccac cacgtccacg tttacttttg aagcgaagtc
     9901 ccacatccgt tcctaaaaac tgacagcaac tgacagaaat atttcttact ccacccgaca
     9961 tttcacttcc aaccacaaaa ccaccatgag ctttgaaaac cttacagttg tgaacaatta
    10021 aattttcggt aggaacagct ctacgacggc cttcttcgtc tttgcccgat ttaatgcaaa
    10081 tggcatcatc tcctacatca aaataactat tgactatgat gacattttta caggattcca
    10141 catcaattcc atccccattt tgagcataat cgggatttcg gacgataatg ttatcaacaa
    10201 tcacattttc acacataaaa ggatgaatgt tccagcttgg agaattttca aataaaacac
    10261 cctgcaagaa aacatttttg cattgaataa aactcaccat aaccggacga agaaaatccc
    10321 ttatttccat ccattcttca tcagtcagtt ttgcacgcgg aacattcatg tcgcttatcg
    10381 agtatccttt gtatgatttt tccgatggaa accaataatt tccctctttt acaactccgc
    10441 ccgaattgac cacttttttc cattccgaag ctgtaacttt tgattttttc aaaggcctcc
    10501 atgcttcgcc agatccattg atggaacctt cgcccgtaat ggctacattt tcaatgttcc
    10561 ttccggaaat gggtgattgg cagcgtttgg ttgccaaacc ttcaaagtag gtatctacca
    10621 aaggatataa atcaaaatct tttgaaaaaa gaattaatgc tcctttttcc agatgaagat
    10681 taatatttga ttttaattca ataggtccag taaaccatac acctgcaggt acaattaatt
    10741 tacctccacc ttttcgggca agagcatcaa ttgcctgttt aaaagcttca gtgtttaaag
    10801 tttttccatc accttttccg ccaaaatctt ctatcctcac cacattgttt ttaaaagtgg
    10861 ggggctggat ttttggcatt tcaaaaggta aatttttgaa aagatattcg taaggatttt
    10921 ttgcgtttaa atgggcagtg attaaaattg cacttgccag cattaaaaat aatgacttaa
    10981 atttcatcac ttttaagatt tagagatcaa taaaatcgat tttttaaaga agcataaaaa
    11041 cagcaataaa actgtcttta tgcttctact cagctatgaa aaaactacca ggaaatattg
    11101 gctccaatac cttgttccaa caaggtagca tttgttatgg ttaaattacc gtttgcttca
    11161 tcaacaaaac cgggattttg ctgcaggtaa gtgcccgaat tatcagtttt tacattcagc
    11221 gctgtacccg tataaacaag ataattgggt gcattgaaat aattattttt gttgaaaagt
    11281 acaatattgg tagctgtctg gttggtataa tatcccactg aattagccac tacattgttg
    11341 gtaaatgtaa tttcattatt agcaaatcgt acgtataata agcgacgagc agcatcatta
    11401 tttactttac taaaagtaca acggttgact ttaataatgg tcgactttcc agggaagctg
    11461 gaagaggcat catcaagccg gaaaaaatca cggtcgggag cacaattata aaaagttgaa
    11521 ttcaataacg ttgcattttc aatacatcca gatcgaacat caatgcaatc ggcactactg
    11581 gtcaaaatgt tggatacttt gcaattttga atggtaaatg atcttaccga aacagcaatt
    11641 ccacttgcac ccgaaatcaa agatttacca taatttgtaa taatacaatc cttgaataaa
    11701 atatcaccac aagccacact ttgagtgagc tgcaccacat gatcacgcat atcagcatcg
    11761 ctctgaccat tcagaataac gtcttttaca gttaaagaag cgttttctgc agttaatcta
    11821 aattgcatat gaagagtagg tttattgctc ttcaaatatc ctctgatact gatacctttg
    11881 tctatattaa ttgtttgacc ggcacttttt aaaaattcat tgcttccatc gtctgaggca
    11941 actaacacaa ttctgctgcc ggaagctgca ccctgaatag caacaaccaa atcttcatca
    12001 ggatgaacaa caatggtatt tgctgcattt aaatcaattt tggtagtaaa agtagcactt
    12061 ccgcgtactt tgtcatcttt catcagcctg gcagtatagt cggtatcttc aatcaatccg
    12121 cttagggtag cttctccagc tgctatttcc tctgccgtta ccacatgctc taaagtactt
    12181 tggccagccg ttaaaataat tttcgtagct gtctctccgg ctttccatgt caattttaca
    12241 ctgtgtgctg aaaccgattc ttcggatacg gcgttcataa tttgttcttc gccggtttta
    12301 aaatacacag cactccattt cgattctgca atttcacttc ccacggcctt gatacgtact
    12361 gaatattgcg ttgctcctat caatccactc ttaatggtat ccggaatatc atcattcgta
    12421 atattagtaa aaatcattac cggacttcca gcaaatgtca agctgtcatt ttcatatact
    12481 tcaattacat aactttctgc ctttggattt tttgtccagt taagaaccac ctgtgttttg
    12541 tttatcactt ttgcttcaac accaacagga gagaacaatc tgtctgtcac caattctgta
    12601 atttcttccg gtaaatcatc tttgcatgaa cttacagtga agacaatcaa taaactaaat
    12661 aggataggaa tatttttaaa tatctttttc atgttgtaga atttaataag gttagttaat
    12721 agccataatc atttttcagc aagccgttac tcgaatcgat aaacacttgc catatgggcc
    12781 aaaattgacg agtatctgga tctctttgaa aaagagaatt aattttatca tcagttaagg
    12841 tgttgacacc attgcttaca aaccaaccct tgctcgattc gtagtttaaa gccgctcctt
    12901 cggtatcggt atgtccatgc tccaatccgt atatgataag cgattcacca tcgctggctg
    12961 ttttatagta aattttttca ggtaggtcgg catattcgcc ttgacgatta gcaagtcggg
    13021 tgagtttagc ttttgcttca ttcatttttg tagaaagtaa attccagcga atcaaatcgg
    13081 ccttcctcaa cgattcacca caaaactcca gagcacgttc gttgacaata gcattgaaaa
    13141 aagcttcttt gctggcagtt acgcttgcca tgtaagcgtc aactttttca ggattatttg
    13201 ggaaagcccg tttgcgaatt tctctcaaat aaggtgctgc tgcttccgga ccatcaagtt
    13261 cgttaattgc ttcagcagcc attaaataaa tatcggcata acgcatgcat tgccaattaa
    13321 ttccatcatc attggtcgaa gtaaccactc ttttcatcca ctcgtaacgc aattttccaa
    13381 aacaccacga actcagtttg cgtggcactt gtttgccatt tgtccattca taaggcacac
    13441 aagtaatatc acgacgaata tcatctttat caaaatcgta ataaacataa ggaagcggtc
    13501 cattctgtcc accttgtgct tgttgagtgt atttatcggt agtagtatgt tttactccaa
    13561 atgtatataa cacccgacca cgtccatcgg aaaatggtat ttcataaagt gattctaatc
    13621 ctgcagtaac gttgtcttga caaaggtttc tgaaattttg ttcaaaagtt cccaatttac
    13681 aggtcttgct ttcaatgaca tcaatacatt cttgtttaac gattggataa aggttttcaa
    13741 ccgaaagttc aggatcatta cttctgcgaa tcgttccatc gggacgttga gaatatccag
    13801 cagcagccag acaaattctg gctcttaaac cttttacaaa agccttgttt actcgttcag
    13861 tggttcgtgt aacagccgtt tcattaggcc aagccacata attttctgct tcggccaaat
    13921 cggcaattat ttgcttgtaa attacatctc tgtccgattt tggcaaataa agcgtagaag
    13981 gagaaatggg ttcaaaccgc gcagggacat caccccacgc ttttatcaaa tcattgtaaa
    14041 tcatggctct taaagtaagg gcttcgccca gtatttgggc aagttctgga tttttctcca
    14101 catttccata aagtcgcaaa cctctaatac ataaattagc acgttcaata gcttcataca
    14161 ttttagccca tgcattgttt tccatattca tctgagtatt ggtaggaagc ggatcgtaac
    14221 cggtcagttt tgcacggtca tcacttacat tttcagatga attgtaccac tcgatatcgg
    14281 tgttaatccc gtaaaaaggt atgtaacgac cacgatagga attggtttct gccaaagatt
    14341 gatgaatgcc catcaaagct ccttctgcca aaacaggcgt agaaaaaatt acctgttcgt
    14401 ccaatgacga tttggtggga gcttctaaag tttcctcgca tgaactcatt attcccatta
    14461 agaaaagagt gagtattgca gaaataaata ttgtcttttt cattttatca aattatttac
    14521 aaggttaaaa atttaaattt aagccaaata ctacctgtcg gctttttgga tatgccgaat
    14581 aatcaaccga tggtgtcaat tcggttttac gtcgagttga aacttcagga tcgaaaccgg
    14641 aataatttgt ccagcaatac aaattataac cggtaacata aaaacgcaac tgttgaattt
    14701 tgatttgtct gattaaatga accggtaaag tataacctaa agtaagcgta tttaacctca
    14761 agaaagaacc atcttcaaca gcccaatcgg aaaaaacata ttttgacata tacggagacc
    14821 acattgttgt attggcattc atggcttcca attctgccgg attgttgcta atggttccgt
    14881 cagatttcaa attagtccag cgttttccgt ctgccatgat ggctaacatg tttcgatatt
    14941 gatatttact ggtagaagtg tattcgattt tattggcatt gtacacatca ttcccataac
    15001 tccagttaaa atttgcagaa aggtcaaatc cgtacaatct tccattaatt gtaaatccac
    15061 cagtatgtaa gggattggca tttcctatta tcgtataatc ttttgaattt acaatgctgt
    15121 cattggtaat gtctttcagt ttcattgctc cagggcgaag cgcatctgat ccaattacgg
    15181 aagaacaatc aacaactcct tccttcaata tccatttttt ctttacagaa tcgtatcctt
    15241 caaaatcaga tacttcatat cttccatcgg aacggtagcc atacatttgc ccaacagaac
    15301 ctcctgcagc aatccagtaa tcgtttccta tttcagttga agcccagcca ctggctgctc
    15361 caaagtcgct cattattccc agagaattga ttttgttttt attgaatcca atgtttccac
    15421 tgaaactcaa accataattc tttttgtcta ttgctatcca attcagtgta aattcaacgc
    15481 ccttattttg cgtttctccc atattacggt actgagtatc ataacctgta ccaggaacgg
    15541 ggaaattgat caacaagtcg gaagtggtat tataataacc ttcaattgtt ccatttaatt
    15601 tacttttcaa gaggacaaag tccaatccca agtttcgcgt tacggttgtc tcccatttca
    15661 aatcgggatt tgccataatt tttgagggag accagtaaga agcaacgtta ttgatccatg
    15721 aagtagactt ggaatcgaaa gattgtgtca tctgaccgga tggtatgttg ttgtttcccg
    15781 ctgttccata gctcaacctg agctttaaat catccaacca agattgagta gattccataa
    15841 aaggttctga agaaattctc catgctaagg cagcagaagg aaaatatccc catttattgc
    15901 ctttggaaaa cttactcgaa ccatcggctc taaatgttac actcatcaga taacgactta
    15961 aataatcata atttgctctt ccaaagaatg ataaaagtat atcgtcgggt gaataataat
    16021 tctcgatatt atagggagtt ccttgagagg ttaacctaaa agcattggta gaagtaaagc
    16081 ttttgggata tccgtgaata gtattagtta acgtttgctt gctattaaaa atgtattctt
    16141 ctcccagcaa tacatccaaa tgatgactcg ttcccaataa tttcttaaag tcataactta
    16201 ccgtattggt atttctaaaa gtattcaaaa atgtattgtt caattcggca gcgggataac
    16261 cctgattttc ggaggaagga atatttttaa tgtaataagt ggtagttcca taaaaacgct
    16321 ggtcttctgc acgataattc tcgtatccaa actcaacttt cagcttaaaa ttatcaaatg
    16381 ccttccacgt aatatcccct gccatattaa gattgttccg cttttgttga cgatcgttat
    16441 cggaaatagc caccaatgga ttatataaat ttcctatttc cgtatcatca ctggtaccgg
    16501 cttctgatgt cagatccttc aaaggaaaag gaggataaat catggcatat ttcaaacgcg
    16561 aatcggcact tgaaacttcc ttttgttcat tcatacctcc accattgatt tttgtgttga
    16621 tgtatcgagt agaaaaattc attgaaacat ttttggaagg attggtagtg tacttaaaat
    16681 taatattgtc acgcttgaaa cttgacattt gcataatggc cttgtcatct atatgactga
    16741 aactaaaaga aaatttgttt ttggaatcgc ctccgttgat gcttaaattg tgattaaaag
    16801 ttgttcctgt cctaccaaat acaagatctt gccaattgtt ggaaggtaca ttgttataaa
    16861 gatcaatatc ctgataattg ccaaaatact tttcgtaaga gcttgcatct ccatcgtttt
    16921 tcaacaaagc cagctcatac tgccacttag cataatctga tggagacaaa acgtccaatg
    16981 ttttggctat tttttttgct gcggtataga aattataatt tgctgttatt ttgccttctt
    17041 ttcccgattt ggtagtgatg ataactaccc cattagcacc acgagaacca taaattgccg
    17101 tagatgaggc atctttcaaa atgtcaatag attcaatatc ataaggagaa atgtcatcaa
    17161 tagattgtac aggaaatcca tcaactatca acaaaggttt gttgcttccg gtaatggagc
    17221 ctccaccacg aacacgtatt ctgatttccg catcgggaga tccttcagta gttgtaacct
    17281 gcacaccggc caattttcct gaaattgctt ctgctggaga agatacggga atgttcataa
    17341 tcgcatccga actaaccgaa actacagaac cggtaatgtc ttttcttttc accgtgccat
    17401 atccaatggc aacaacttca ttcaatccta tcgcttcttc ggtcattacc acattaatga
    17461 cgcttcgatt ccctatttca acagttaacg gtttcattcc aacataggaa aactgcaact
    17521 tggtggcatt agccggagca gtgatagtaa aatctccatt taaattggta acagtacctg
    17581 cagaaggaga tcccgcaaca ataactgata ccccaattaa tggctctcca tcagtagttt
    17641 ttacggttcc tttaatcgtt ctttcatttt gctgaattaa attgttctgg tcagtaaaac
    17701 cataaacttg aacaaaaaaa ccaaccaata tgaaaaacaa aatggaaata aatttttttt
    17761 gagttctcat aaaattagat ttaattaaaa gttaagtatt taaaattgat gtgattctta
    17821 tccttctaaa tgtttcaata taaaaaataa tcttttaatt ttatcgatgc taaaattaaa
    17881 atgtgttcgc acacggtttt tatttcaaaa ttatttttaa tgtgttgaaa aagcaatgaa
    17941 atatatgatt atacattttt ttaaacttat tactcgtaat aactatatgt ctctgattaa
    18001 caagtaatta tattgttttt caaaaataat gtgtacgcac acgattttat cacaacaaat
    18061 gtaatatata attctctatt taattcactt tgaaaaatta aataatgtta aatatatatt
    18121 gttgttacat tcttgttttt gtaattctct gattttgtga tttttaaaat aaaattttat
    18181 ttccgaaaaa tttatgtttt gcacgtaagc agcgaaaaat aaaaaaagaa cctctgaatg
    18241 ctcatgctga taaaatatta ccatcttaaa ttattgctaa ataatgtttg atttgcatgg
    18301 aaaatttaat aacataaata aggttgtatt ttgcaattga ctttatagcc cgaattggca
    18361 attatatcat ttgtaaacaa aataaggaat aaaactttga aaatttgctt ttccaacttt
    18421 ttatctatct ttgctcccaa aaaatttgtg gagattaatt tccttaatat tgtgaaaagg
    18481 catcttttaa ttaacttttt caattatttg tagatactat cgctttttta aaaactacca
    18541 atcttgtggt gaatttttaa agaaaaatca aatataatca cttaaattan nnnnnnnnnn
    18601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc taccaatctt gtggtgaatt tttaaagaaa
    18721 aatcaaatat aatcacttaa attaatacgc tatgagtatc aaatttcgct tgattcttat
    18781 gaatttcctt caatatttta tttggggttc ttggttgatt tctttgggta gttatgcagg
    18841 aaatattctt aactttagcg gagtccagat tgggagtatc tttgcctcaa tgggtattgt
    18901 ttcacttttc atgcctggat tgcttggcat tgttgccgac agatggattc cggcacataa
    18961 aatgctgggt atttgtcatt tattaggttc aatatttctg attgcagctt catttcaaac
    19021 ccaatatatg gcattttaca gcttcatgtt cataagtgta atgctataca tgcctactat
    19081 ttcactaagc aacacagtgg catacaatgc tttggaaaaa aatcactacg atattgtcaa
    19141 agattttcct ccaattaggg tatggggaac cattgggttt atttgtgcca tgtggacagt
    19201 tgacttcacg gggttcaaat tttcacaagc gcaactttat gttgctgcta cagcggcttt
    19261 aattttggga gtctattctt ttacattacc accctgcact gttaaccgaa gcaacgaaaa
    19321 aaaatcggta atagatgtat tggggttaag ggcatttact ttatttaagc acaaaaaaat
    19381 ggccatcttt tttatatttt ccatgctatt gggggcttca cttcaaatta ccaatggttt
    19441 tggagactta tttttgagaa gtttctccga aattcctgaa tatatgaatt ctttcggagt
    19501 aagacattct gttgtgttac tatccatttc acaaatatcc gaaacccttt ttattcttac
    19561 tataccattc tttttgaaaa aatttggaat caaaaatgtt atgctaatga gtatgtttgc
    19621 ttgggtattt agatttgcac tttttgggat gggcaatcca ggagacagac tttggatgct
    19681 tattctttca atgattgttt atggcatggc ttttgacttt ttcaatattt ccggatctct
    19741 ttttgtcgaa caatcgaccg attcttccat tcgggcatct gctcaaggat tatttatgat
    19801 catgaccaac ggactgggcg caacttttgg atcctatcta gccggcgcta ttgttgataa
    19861 atttgttacc catgccgaag ttccaaattg gtcaactgcc tggtatattt ttgccggtta
    19921 tgccttaata gtgggaattt tatttgctat tctcttcaaa tacaaacaca acacacaaga
    19981 agttaccgaa aaatttcatt gataaaacac ttttttcatt gaaatgaaac aacgtaaatc
    20041 caagtaaatc aattgttttt tcaaataatg aaagtattcg gttaaattca ccaaattccg
    20101 ttttgtaaca taaaaagggg ccgtctcaaa aaattaggat agccccctct ttatttttca
    20161 agttttttat ttatttgttt ttgaaaacaa taccattttc gtcagcatca acaaccacta
    20221 cttggttgct aacaatctct ccggcaatta ttttcttcga caaatcgttc aaaataaatt
    20281 tttgtattgc tctctttacc ggccgggctc caaactgtgg atcgtagcca ttgtcggcaa
    20341 taaacttcac tgcacgatcg gtagtttcca accggatacc gttttgttcg agcattcggc
    20401 gtacaccatc caattgcaga cgaacaatat cttctatctg cgaacgatca agtggtttga
    20461 acatgatcag ctcatcgatc cggttcaaaa attccggacg aatggtttgt ttcagcaatt
    20521 caaacacctc atttttcgtt ctttcgatga tttcatcatg gttgtattcg ttaatttttt
    20581 caaaattttc acgaatcaat gatgaaccaa tgttggacgt catgatgata attgtatttt
    20641 tgaaatttac agtacgtccc ttgttatcgg tcaaacgtcc atcgtccagt acctgaagca
    20701 aaacattgaa gacgtcagga tgagcttttt ctatttcgtc gaaaagcaca acggaataag
    20761 gttttcttcg tatggcttcg gtgagttgtc caccttcgtc gtaacctaca tatcccggag
    20821 gcgaaccaat aagtcgtgtt accgaaaatt tttcctgata ctcgctcata tcgattcgtg
    20881 tcatcatgtt ttcgtcgttg aacagatatt cagccaatgc tttggccaat tccgttttac
    20941 cgacaccagt ggtaccaaga aagataaacg atccgatagg tctttttgga tcttgcaatc
    21001 cggctctact gcgacgaatt gcatcggcaa cagcctgaat agcatcgtcc tgtcccacca
    21061 ctcttttatg taattcttct tccaagtgca aaagtttttc cttttcactt tgcaacatct
    21121 tggttacggg gattccggtc cagcggctta caatctctgc aatatcttcg ctgtccactt
    21181 cttccttaat caacggatta tttccctgaa tttttgccag ctcttcctga tattgagcta
    21241 tggtggactc taatgctttt attttgccat aacgaatctc ggccactttg ccataattgc
    21301 cttctctttc ggccttttcg gcttcaaatt tcaaattttc aatgtctatt tttgcctgtt
    21361 gaattttgtc aatcagcgat ttttcacttg cccatttggc tcttaaggca tcggcctgct
    21421 ctttcagatt ggcaatttcc tgactaagtt gttcgagttt ttttgtatcg ttttcacgtt
    21481 tgatagcttc acgttcaatt tccagttgct taatgtttct ttcaatcaca tccagttctt
    21541 ccggcacaga atcaatttcc aaacgcaatt tggcggcggc ttcgtccatc aaatcaatag
    21601 ccttgtcggg aagaaaacgt tcggtaatat aacgactcga aagttcaacg gctgcaatta
    21661 tggcgtcatc cttaattctc accttatgat gattttcgta gcgttctttc aaccctcgca
    21721 aaatggaaat agaactcggt atatccggtt cgtccaccat gacgatttgg aatcgacgtt
    21781 ccaatgcttt atccgattca aaatattttt gatattctgc caaagttgtt gcaccaattg
    21841 ctcgcaaatc gcctctggca agtgctggtt ttaaaatgtt ggcagcatcc attgctcctt
    21901 cgcttttccc tgcgcctacc agcgtatgaa tttcgtcaat aaagagaata atttcgccat
    21961 tggaattaac cacttcattc acaaccgatt ttagacgttc ttcaaattca cctttatatt
    22021 tggcacctgc tatgacggca cccatatcca atgagtaaat ctgtttcgat tttaaatttt
    22081 ccggaacatc gcctctcaca attctgtgtg ccaaaccttc cacaatggca gttttacctg
    22141 ttccgggctc tcctatcaaa ataggattat ttttggtacg acggctcaat atttgcaaca
    22201 ctcgacgaat ttcttcatcc cttcctatta cgggatccaa cttgccgctt ctggcacgtt
    22261 cgttcaggtt aatggcatat ttgtttaaag aattgtatgt atcctccgcc gaagccgaat
    22321 ttacttttga gccctttctc aattcattaa tggcttcgct taacgacttt tcgttaacac
    22381 ctgcatcatt cagtgctttt tttacactgt tgttttccac aaccaatgcc atcagaatag
    22441 attccaatgc cacaaattca tcaccagctt ttgaagaaat atcaatggct ttctgcaaaa
    22501 cagcatttgt ttctcgtccc agataaggtt cgcctccgga aacgcgtgga taagtttcta
    22561 tcattcggtc gattacagaa ttcagcatcg acaaattaac gcccattttt ccaaacaaat
    22621 actgaataac atcctcgcct ttcaacaaaa cacctttcag caaatgaggc gtttcgacag
    22681 cctgctgtcc tttgctctga acaatgtcca cagcttcctg aatagcttcc tgcgacttga
    22741 tggtaaaatt attgaagttc ataagtttta tgttttaaaa attttttaac tatttcaatt
    22801 gtttttcctt tttcgttcaa aacacttacc aatcatcaaa agaggaaata ttgacgtgat
    22861 ttttcattca attaactgat aaacaagaaa atatttcata attactgaaa atatgtcatt
    22921 aatgctgcga tagcaaattg caatataagg atgacaattt ccccgttttc ctttccattt
    22981 ttaatcaaaa taattatttt tgtagtttga agttgttcat tcaaactata aaaattttta
    23041 aatgaaaaaa aaatatggat aataagaaaa gagtttacac atttggtaat gggcaagctg
    23101 aaggaagcgc cagtatgagg aatttattgg gaggaaaagg tgcaaattta gccgaaatga
    23161 atttgctggg tattcccgtt ccaccgggtt ttaccataac caccgaagtt tgtacggaat
    23221 attacgcttt aggaaaggat aaagtagtgg aactactcaa acccgaagtg gaaaaagcta
    23281 tggaaggcgt tgaaaaacta atgcgttcca aatttggaga caaaaaaaat ccattgttgg
    23341 tttcggtgcg ctccggagca agagcttcca tgcccggaat gatggacacc attttgaatc
    23401 ttggtttaaa tgatgaaatt gtagaaggct tggctgaaaa aaccggcaat ccgcgttttg
    23461 catgggattc ataccgccgc tttattcaaa tgtatggtga tgttgtattg ggcttaaaac
    23521 ctgccaacaa aaacgatatc gatccatttg aagcagttat cgaacaattg aaggaagaac
    23581 gaggcgtaaa actggacacc gagctaacgg ttgatgactt gaaagaattg gtcaaaagat
    23641 ttaaggcttt ggttgccgaa cgaacgggca aagaatttcc gctgtcggtt tacgaccaac
    23701 tgtggggagc tatttgtgcc gttttcgatt catggatgaa tgaacgcgcc atattttacc
    23761 gaaaaatgga aggaataccc agcgaatggg gaactgctgt taccgttcag gctatggtat
    23821 ttggaaatat gggaaatact tctgctacag gtgtttgttt cactcgtgat gcggctaccg
    23881 gcgaagatgt tttcaatgga gaatatttaa tcaatgcgca gggagaagat gtagtggccg
    23941 gtattcgtac tccgctgcaa atcaccaaac aaggttctca acaatgggca aaacttacag
    24001 gcatttcaga agaagaacga aaagaaaaat atccttcgat ggaagaagcg ctgccggaaa
    24061 tctataaaga actgaatgaa gtgcaggaaa aactcgaaaa acattacaaa gatatgcagg
    24121 acatggaatt caccgttcag gaaggcaaac tctggctact gcaaacccgt aatgccaaac
    24181 gtaccggaac agcaatggtg aaaattgcca tcgatatgct tcatcaagga attattgatg
    24241 aacaaactgc cttgttgaga gtcgaccctt tgcgtttgga cgaactactc cacccaattt
    24301 ttgacaaaaa tgccgtaaaa aaagcaaagg tcattgcaaa aggactggct gcttcacccg
    24361 gagccgccag cggaaaaata gttttcaatg ctgatgacgc tgcagaatgg gctgcacgcg
    24421 gagaaaaagt tattatggtt cgtatagaaa cttcgcctga agatttagcc ggaatggccg
    24481 cagctcaggg aattcttact gctcgtggcg gaatgacttc acacgccgca gttgtagctc
    24541 gcggaatggg aaaatgttgc gtttcgggtg ccggttctct taaaattgat tatgctaaaa
    24601 aaacaatgga agccgatggc attgtactcc gagaaggaga ttacatatcc ctaaatggtt
    24661 caacaggtga tgtttatctt ggccaaattc ctacgcagga agccgaattg gatgccgatt
    24721 ttgccgaact gatgacgctt gccgataaat attcgagcat gaaagttcgc accaatgccg
    24781 atacgccaca cgatgcagcc gtagctcgaa aatttggtgc ggttggcata ggtttgtgcc
    24841 gtacggaaca catgtttttt gaaggagaaa aaatcaaggc catgcgtgaa atgattttgg
    24901 ccgaaaatga agaaggacgc cgcaaagcac tgggaaaaat tctgccttat cagcaagctg
    24961 attttgaagg aatttttgaa gtgatggatg gttttccggt tacggtgcgt ctgctcgatc
    25021 ctccattgca cgaatttgtc ccccatgatg aaaaaggaca gaaggaaatg gctgaagcta
    25081 tgggacttcc cttacaaaaa atacaacaac gcgtcgaaga acttcgcgaa caaaatccca
    25141 tgttgggaca tcgtggatgt cggttgggaa acacttatcc agaaattacc gaaatgcaaa
    25201 cacgcgctat tttgggagct gccttgaaac ttaaatccaa aggaatcaaa atcatacctg
    25261 aaattatggt acctttggtt gggataaaaa aagaatttct ggaacaggaa cgcataatcc
    25321 gagagactgc cgaaaaactt tttgccgaga aaggtgatcg tatcgaatac aaagtgggca
    25381 ccatgattga agtaccgcgt gcaacactga ttgccgatca gatagctgaa aatgctgaat
    25441 ttttctcgtt tggtaccaat gacctcacgc agatgacttt cggcttctca cgagacgata
    25501 ttgcctcttt tgttcctgat tacatcgaga aaaaaatcat tgaaaaagat ccgttccaag
    25561 ttctcgatca ggaaggtgtc ggacagctta ttaaattagg agttgaaaaa ggacgttcta
    25621 cacgcagtga tttgaagatt ggaatctgtg gtgaacacgg aggagagcca tcttccattg
    25681 aattttgcta tcgcgtggga atgaattatg tcagttgttc tcctttccgc gtgccaattg
    25741 cacgcttggc tgcagctcaa gcagctttga aaaattgagg tggcaaaata cgattaaagt
    25801 taattcgaaa acacgcctac tcttattctt tgcaccgtgc atgctttatg cacggttttt
    25861 ttgcgttttt ccattattat tttctttgaa ttacatggtt tgtggtaacc tttttatctt
    25921 ttgggtgaaa aatttatcct ttttacacgg attggggaac aagaaagcaa ttacttttgc
    25981 aacaatttca agcattattc aataaaatat caaacggcat tttaagcaat caagaaaaaa
    26041 aagaaaaaat caagtcaata aatccgactt tttatctgtt gttttcatta ctgatggtaa
    26101 tagggttgtc ttttgcagac agccttattt ttttgtagca gcgccacgac gtatcgggtg
    26161 caaaagcgta aaaaaattgt tgtttgttaa ggctgtaatc aaaattatat ttacaccatt
    26221 aataaattga tttaaaaaag attatgttta aaaggttaag ttttaatttt aataattcat
    26281 catttcaaat tatctttgta ctaaaaattg gttgtcaata atggaacaaa gtgaacaaat
    26341 agttaataaa attgttgaag gaatacaaga aaaaaaagga aagaaagtag tcattattaa
    26401 tttaaccggg ttgaaaaatg cgccttgcag ttattttgta atatgtgaag ggacaacttc
    26461 gatacaggta gatgccattg ggacttcgat aaaggataca gtaagagaaa aaatgaatat
    26521 aaaaccttat gctatagatg gtttaaggaa tagcgaatgg gtggccatgg attacgggtc
    26581 ggtaattgtt catatttttc ggccacagat tcgtaatttt tacgatattg aacatttatg
    26641 ggaagatgcc gaaataacag aaatttacga tgaatgataa aagcaaaaca acaatttaaa
    26701 aatattcaaa caaaaaaatt aaagagaatt tacaatggaa accaaatctc cacaaaatag
    26761 tcctcaaaaa cccggcaaag gaccaaagtt taatattaca tggctttacg tcatcataat
    26821 aggtattctg ataggttcat atttgttttc ggatactgct ccagtaaaag aagtcccatt
    26881 ttctgatttt gaaaagtaca tgtataacaa caaaatggaa aaaattgatg tgtattcttc
    26941 taaaaatatt gctgaagcac ttatcataaa agattctttg aagcaggttt ttggcgaaaa
    27001 atatgaaatg tacgaaaaag aaccaaaaat caggacaaga attccttctg cagaagagtt
    27061 ttcaaaaata tatcaaaaag ccaaagagca atatggcctt acagctgtgg tcgattataa
    27121 ggaaagccgc aattatgtag atattttcct ctacagtatt ttaccttttc ttttgctgat
    27181 attgtttttt gtttacatga acaggcgcat ggcaaatcag atgggcggag ggtcaggagg
    27241 aggcattttt aatgtaggta aatccaaggc acaacttttc gataaagggg atgtggccaa
    27301 caggattacc ttcaaagatg tagccggatt ggaagaagcc aaacaagaag ttgaagaaat
    27361 tgtggaattt cttaaaaatc catccaaata tacggaattg ggaggtaaaa ttccaaaagg
    27421 agttttattg gttggtcctc cgggaacagg aaaaacttta cttgcaaaag ctgtggccgg
    27481 agaggctgat gtgccttttt tctctatttc aggatcggaa tttgtagaaa tgttcgtggg
    27541 tgtaggagct tcacgtgttc gagacctttt ccgacaagca aaagagaaag ctccctgcat
    27601 tatttttatt gatgaaatag acgcgatagg acgtgcaaga ggtaaaaatc cgaatttcgg
    27661 gagcaatgat gaaagagaaa atacactcaa tcaactactt accgaaatgg atggttttgc
    27721 ttcgaacagt ggggtcattt tgttggcggc aactaaccgt gccgatattc ttgacaaggc
    27781 gttattgcgt ccgggtcggt tcgacagaca aattcacgta gatcttccgg atgtacatgg
    27841 aagaaaagaa atatttaaag ttcatttacg tccaataaaa atcgacaaat cggtagatat
    27901 agatttttta gctaaacaga cgccggggtt ttcgggtgca gatattgcca atgtttgtaa
    27961 tgaagctgcc ttaattgcag ccagaaaaaa caaatctttt gtagaaaaac aggatttcct
    28021 tgatgccgtt gacagaataa taggaggttt ggaaaaacga accaaaatca ctacaaaatt
    28081 tgaaaaaaaa tccattgcat accatgaagc cggtcatgca accatcagct ggatgctcga
    28141 acatgcaagt cctttggtga aagttaccat tgtgcctcgt ggagaagctt tgggggcagc
    28201 atggtatctt cccgaagaac gtcagcttac cgttagagca catatgcttg acgaaatgtg
    28261 ttctacattg ggtggaagag cagcagaaga actattttta aatcaaactt ctacaggagc
    28321 cattaacgat ttagaaagag taaccaaaag agcttatgcc atggttactt acttcggaat
    28381 gagcgaaaaa ttacccaatt taagttatta cgattcaagt ggttcagccg agtattcgtt
    28441 tacaaaaccc tatagcgaaa aaacagccga gttgattgat gaagaggtta aaaacattgt
    28501 cagtcgagaa tatgaacgag ccaaggaaat tttacgggaa cacgccgaag gacacaacag
    28561 actggccgaa ctattgattg aaagagaagt tattttttcc gaagatttgg aaagaatttt
    28621 cggtaaacga ccttgggcat cacgcagtga tgaattgctg gctcaaaacg gggaaaatgg
    28681 gaaatcatcc gaacggatgg aagacaatac cgttgaaaat gaaaataaag aagaatgatt
    28741 tgattaatga agaagaacta tcttcgcgag aaatcatttt aaaaaagatt tcggatattc
    28801 tttgcacccg tacatcagaa actatttttt cagaatattt ctcacatcag atttataggg
    28861 aaattgctcc cgatatgctt acctgtttta tggaagaaat aaaggcagta tcgggagaat
    28921 gttttgtcta tgacagtcca tcgaacttat ttgttgccct gaaaaaattt ttgttgggaa
    28981 agggattttc caagatacat tgtcgtaatg aaaaaataac ggaatatttg aaaagttttg
    29041 atattccttt ttcatcttcg gaagccgatt ttgaaataat ggaagtgggt attacggact
    29101 gtgaatttct ggttgcccgt accggaagtg taattgtttc gtctttcaat tccggaagaa
    29161 aaatgaatat ttttccacct gtgcatatag ttatagctca tcgttcacaa cttgttagtt
    29221 atctcgatga tgcttacagg caaattcttc agaaatacaa aaataatttg ccatcactta
    29281 tttctaccat aaccggccca agtcgtactt cagatatcga aaagactttg gttttaggag
    29341 cacacgggcc aaaacaattg ctggtcttcc tgttagacaa ctaagacgct gtttttcttt
    29401 ataataaaaa tattgtgtaa gtttgtaatt ttgaaaacat aaatccatat gaacgaaatg
    29461 ttaaaacgag caattagcgg ctttatttat gtaggattga tagtaacaag cattcttgtc
    29521 catccacttg cttttgcttt tgttttcggc attcttactt attgcgcact tcatgagttt
    29581 tacaaaatca cctatcgacc ttcagatata gaagttccca tttctcttgc ttcaatgggt
    29641 ggcttacttt tgtttgtttc tacttttttg tttaaatctg gttttagttc acccctcatt
    29701 ttttcttatt atgcaatttt tgttatatgg atgtttatca gggaattgtt cctcaaaaaa
    29761 agtaatccta ttcataactg ggcatatttt atattgggac aggtgatggt ggcactgcca
    29821 atgtcgctgt tgatttttat tttatttgta gatggttatc aacccatgct tttgttgtcc
    29881 atatttattt tgacatggct caatgatacc ggggcctacc taattggcgt taagtttggg
    29941 aggcatcgat tttttgaaca tatatctccc aaaaaaacgt gggaaggttt tttcggagga
    30001 atctttttta gtttaacggg aggatttata ttatctcttt ttatcgctga actgagcatc
    30061 tttcaatggc ttgccctatg ctttataaca gtattggcag ccaccttagg tgatttaacc
    30121 gaatcattat tgaaaagaac tttaaacata aaagactcgg gaaacctttt acccggtcat
    30181 ggtgggttac tcgaccgttt agacagcatg ttgttggttt caccctttgt tttgattttt
    30241 tttctttata tttttgtttg aatttgctgt tctattgtct tttatgaata ataagtaaat
    30301 ttattgctga gaaaataaaa ccatattgaa ttgaaaataa acagaatcta tggaaatgaa
    30361 ttttgatgac atacgttgct atacaaacga ggaggtacat gaggtgttgg aaaaactaac
    30421 caaagaaaaa tcgttcatga aagtggttag cacagcttac cctttgctac ccaaagaaat
    30481 aattaaagaa cgattacttc aatataacaa tcaacaggaa tttcagaaag attttatttt
    30541 tccttttttg cagtatcttg aagaaaacac taccaaaggt attgaattgt tcggtttgga
    30601 aaaaatcaat gtctctaaag cttatttata cttatccaat catcgcgata ttgttatgga
    30661 ttcaagtttt ttttgcaaaa aattattgga aaattcaatt gatacagtag aaatagctat
    30721 aggagataat ttgcttattt acccgtggat tgaagatttt gtgaagctca acaaaagttt
    30781 tatcgtaaaa cgtgggctga atacacggca aacgttggaa agttccaaat tactttctgc
    30841 ctatctgctt catactattt gtgaaaaaaa tcagtcgata tggatggctc aaagagaagg
    30901 aagagcaaag gattcggacg actatactca ggaaagtgta ttgaaaatgt tgaatttaag
    30961 tgcaaaagat aataatttta tccagcatat tttacaattt aatatttgtc ccttatccat
    31021 ttcatacgaa tacgatcctt gtgattacct gaaagccaaa gaatttcaat taaaaagaga
    31081 cttccccgac tacaaaaaat catcaacaga cgatttggaa aacatggtta tcggcattat
    31141 gggatataaa gggaaggtgg tgtttcgtat taccggctgc atcaacgatg aattaaaaaa
    31201 aatagaacaa aaaacatcca acaggcacga acaaatttcc caaacagcac aattgataga
    31261 tctaaaaata catgccaatt atgaaattta ttcggtgaat aaaattgcat tcgatacgct
    31321 tactggtgaa aatagattcg ctcatgaata ctccaatatg gaaagaattg attttgaaca
    31381 gtatttgatg aagcaaatag acaaaattca actggaaaat aaagatgaaa aatttttgag
    31441 gcaaaaattg ttggaaatgt atgccaatcc cttaataaat catttgaaga ccttgcaaca
    31501 ttctcaacct tccgtataaa aaagaaaaaa tagaccggaa cgattttttc aacacaaaaa
    31561 atcgaactta ccaaattcac tctccataga tacaatgttt accatttgta atcatcagtc
    31621 attattacaa atgggatggt gtttaccata attttttatg gaagttgtcc tgaaattgtt
    31681 tgaaaaatat gcaatcgttt gcgttagttg aaaaaatatt ctacatttac caccgaaaaa
    31741 accaaaatta attttttaaa atgttaatat catgagaaaa ctactactgt tattggtttt
    31801 gggtgctcag acaattttcg ctcaaaacat tcaggttaca gctgtttctc cgataaacac
    31861 cggagaaagt ggcggcatgt atcatccggt atttagtccg acgggagaat atttgctttt
    31921 cacttctgaa aattatgaag gactgaaaat gctttcgttc actgatgggc aggtaaaaac
    31981 tttgacaacc gaaaaaggtg cgggctatgg agttaagata agcaaagatg gaaaaaccat
    32041 tgcctacagg aaaaacgaat tgattaacca gcggctattt tcttccgtta aattgtattc
    32101 gctttctgaa ggaaaacaac aacaacttgt tgctcccacg cgagaacctc ttgcggtagg
    32161 tttttcagga aacaaacctt tattggtaaa aaatcgtcaa ctggtaaaaa gtcagctttc
    32221 tgccgtcgaa attgtaccta ttgttaccat cgaagaccgt aaaatagttc tttatacggg
    32281 aaattcgcgt aaaattctta ctccgaatgg tgaaaatgcg agctatattt ggccttccat
    32341 ttcgcccgat gggaaacgaa ttgtctatgt tgctgcagga aaaggaacgt ttgtttgcga
    32401 cttgaacggg aacaatgttg cggctctggg taaactgaat gctccacaat ggctcaacaa
    32461 tcaatggttg gtgggaatga atgacattga caacggggaa atactccttt cttcttctct
    32521 ggtagcagca tcgatagatg gaaaagtgta tcaaacactc actacacctg atttgatagc
    32581 tatgtatcct gctgtttctc ctgatggaaa gcagattgta tttaatactg agagaggtga
    32641 tttatattta cttaatgttg ttttaaaata aggaggagga aacaccatga aaaaaataat
    32701 ttcatttttc ttcgttttga tgctgatttt ttctgcaaaa gctgttgatt ttacgggaat
    32761 taaaatttat atcaacccgg gtcatggagg atacaatggg gcaaacgaca gaaatgttca
    32821 gaccataccc tttgcattgg gcgacacact tggtttttgg gaatcggcaa gtaatttgac
    32881 caaaggattg gcactacgcg atttgttgca ggcagccaat gctaccgttt acatgtcgcg
    32941 tactcaaaac agggaagaag acgatagggc tttgtcggaa attgccgaag aagccaatgc
    33001 caacaacgtg gatgcctttc tttccataca cagcaatgca ttgggaacca atacaggcac
    33061 caattattta ttacttttgt atcacggtta tgatgatcaa cccactgtgg cagcgagttt
    33121 accaatggct caaaccgcct ggcctcgttt gatttcaaat caacttacag tttggacaca
    33181 ttacaccacc tctactaata ttcggggcga tttctcattt tatggaaata ccagtggact
    33241 gggcgtcctt cgccctttga ctgttcccgg atttttgtcc gaaggttcat tccacgatta
    33301 tttaccggaa acccaccggc tgctcaataa agattacaga aaattggaat ctaataattt
    33361 ttacaggtat ttctgcgatt attttcaaac caatttacct tctacaggta ttattgcagg
    33421 gtttgccaaa agttccaatg aaaaaatgaa caatcctttg tacatctaca aggcttttac
    33481 ccatgatgaa aaagtaccta ttaatggagc taaggtgaaa ttgatgaatc agaatggaga
    33541 ttcactgaat gtatattatg tagatacgct gtacaacgga atttttgctt tttacaattt
    33601 agctcccgga acttacaaac tgcatttgga agcaaaggat tatcagccaa aggatacaac
    33661 tgttacggta acggcagcat caactactta tgccaaaatg tttttaccta acttaaatgt
    33721 ggtaattcca aaagatacca cgcctgatta tcctgatccg atacaggaag ccggtgtact
    33781 tccaatgaag cattataatt ttggagatca agaagtagtt gttccagaat ggttgaatca
    33841 gcaacctatt aaaaaagttt tgtatcgtga tgaaaaatta tatgtgctaa ccacagaacc
    33901 aaaaatcact atttacaatg cgcaatcact tgctaaaatt cgcgaaatgg atttatccgg
    33961 cattgaaagt ggcgtaaacc tggttagcga tatcgctttt acttccgatg gctatttgtt
    34021 ggcctgcaac aaagatacca tatctctacc cgagaacaag ggacgttatt ttaaagttta
    34081 cacatgggat aacgattcgg tagctccttc attgcttttc aagacgcaac aacaggcaaa
    34141 ctggtcaaat ggcattatgg gcgaaacatt tgctgtaagc ggtccccgct ggaaatgtac
    34201 tatttacgta ccatcagtta ccaccggatc atccaaagcc attcgcattg taggttacct
    34261 ttatgaagaa ggaaatgcct tgggctacaa atatatgatg gatgctgcca attacaccga
    34321 agcattgtgg ggagaaaata tgaaatttac tatttctcca agtgggaaag atcattttat
    34381 tctggatagc gaaaaagttt taccagtcga atatcagttt gattggaatg cagccgatcg
    34441 ttctccttta attaaaaaag gtgaattttc agaacagaat ggattcaacc ttgctgtctg
    34501 tgcttccggc attcatttct tccgccatgc acaacatacc tttatggttt cgcctacttg
    34561 cgatgccgat acaacgaaag tgggtgctgt gctctttgat gtaaccaatg gcattcagca
    34621 atcgttgaaa gtttccgaaa aattgcctgc taatgggctg gggactgcaa aagccagtta
    34681 tatggttgct gctgcaaaag tcagcgggta tgatctggac gtgatggtgc tggcacaaaa
    34741 cgaaggaatg gctcgtttca gaactgttgc cggacctgcg gtcgccaata tttatgcttc
    34801 agaattgaaa gtcgaaaaaa ccgaaaccga ctttatgctg aaattcacat tgaatgaaaa
    34861 tgctacctcc gtaaaaattg atattctgga tggtgaaaat atcattaaaa ccattgatgc
    34921 tggagctttg aacaaaggag tacagtctgt tcccgttcct ctgacggaat tgccctcagg
    34981 gtctttcact tggagcgtca cggcaagcac tgaagccgtt gatcgtcctg taaaaatttc
    35041 tgatgattcg cgtcctgaac ttcaattcta ttcaccacgc ggcgtggctg tcgataataa
    35101 ttttgagagt cctttctttg gacgggtcta tgctaccgaa actgtaggtg gtaacacaac
    35161 caatcgtacc acacaggatg gtgtgtatat tttgaatgca gccctcgaag atgttacgca
    35221 gcagggcgct caaagttatg ccggttcggt aagctgggca ggcagcagca gtccaatgcg
    35281 actggcagtt gctgaagacg gtaaggtata tgtaaccgat tggagcgatg cccatccggg
    35341 cgtttggatc atggacccgg cggctccttc tgctcctttt gttccagtct tttccggact
    35401 gaccaaagct acaagcggtt tgtcttcaat taatggcgtc aatgtacatg gttctatttc
    35461 tcattgctgg gttttgggca aaggagaaga tactcgttta tatactttcg atgaagatta
    35521 tgtggatgca gtggcaacca gcgccggcaa tgtattgcaa tacaacattg gcacgctggc
    35581 tacttcatgg cagcaagctc ccagtgccat tgtttacaat gatgcgctga atggcaactt
    35641 gcaacagaat tacaattcct gcattgctcc cgatggccgt ggcggttggt ggatttctca
    35701 atatcgttct gccgatgcag ccaatatccc ttcgttgatt catgtcaata cggatggact
    35761 ggtagatttt aactccggaa aaacaccgac gttgattgaa aattcctata caggcggcat
    35821 ggcagtaact gttgatggaa aaacgctggc tatgggctgc aacaatgaag taaagatttt
    35881 tagtgttact ttctctgaag atggcatccc ttctttaacc cgactttact ccattaagcc
    35941 ggctatggga accaatactg caggtttggc tttcgacagg gcaggtaatg tatatgtcat
    36001 ctccaattct acggaaaggc tgggtgtgtg gtcattgcca aaaatagaga atgtattcac
    36061 cacaccggct cccgccagcg aaaaattaga tgtaatcagt gaggttccgc agcttgaaaa
    36121 ggaaaaaaca gaaattctgt tgtatcccaa tcctgcaacc gatcatgttg tgataagcag
    36181 taagacagct cacctgaaaa caattgctgt ttacgacacc aaaggaagat tgatacgcgc
    36241 aatgaatgcc gccggaaatc aaatgaacct gtcgctggaa gggatggctc caggattgta
    36301 tattcttcag gtacagactg acgaaagact gaattctttc aggctaatca aacgataaaa
    36361 cttgtttatt cttttatttc ttcgtgtggc agccttacga ctactacacg aagaaataaa
    36421 aatatgttac aaaacataaa aaatattttt gcaaacgttt gatatttaaa aaataaatgt
    36481 gtacatttcg caactttatt cactttaa
//
LOCUS       DAOSIB010000013        34914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_1417_length_34914_cov_13.534955, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000013 DAOSIB010000000
VERSION     DAOSIB010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 34914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34914
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1417_length_34914_cov_13.534955"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(897..1556)
                     /locus_tag="PLD44_02235"
     CDS             complement(897..1556)
                     /locus_tag="PLD44_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOF98188.1"
                     /translation="MKRFIYSAVMLFFSVSVFSQIDFGVKAGYNSSVGLNELSSVTSG
                     NYNVDSIRADVKNNFQLGVFGRLNFGKIYFQPELLYTMGKKEYTVTLQDTQNQSASYD
                     KVVNISNIEVPLLVGYKVIDLIKMVNVRVFAGPKLTFNAGSSLKFENLKNITAAELEK
                     DVKNAQLGLEAGLGVDVLKFTLDARFNVIKDMYQTKIQDVTVDDFQANTIVISLGWKL
                     F"
     gene            complement(1647..2567)
                     /locus_tag="PLD44_02240"
     CDS             complement(1647..2567)
                     /locus_tag="PLD44_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOF98189.1"
                     /translation="MIRKFFVLFFGFNIFIDSLLWAIDPVTALPIPKQVVFANETVSL
                     ERIDMRERYDREQTVIAYNHSTSILLLKRANRFFPVIEPILKNNGIPDDFKYLAVVES
                     NLDLRAFSPAQAAGIWQLMPKTAEQYGLEVNDQIDERYYLEKATVAACQFLKSAYAKY
                     HSWVTVAASYNAGMAKISGEQEKQQVEDAYDMLLSTESSRYIFRILAMKQFFENPKLF
                     GYQLKKDDFYPYIPTKEIVVSTSVEDWTEWAKQYHLTYFQLKEFNMWLRDRKLTNPKG
                     KAYRIQIPDTADLKFNPQHLKIHQPIWAVW"
     gene            2926..3378
                     /gene="coaD"
                     /locus_tag="PLD44_02245"
     CDS             2926..3378
                     /gene="coaD"
                     /locus_tag="PLD44_02245"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOF98190.1"
                     /translation="MKRAIFPGTFDPFTIGHYSIVKRGLKLFDEIIIGIGVNQAKKTL
                     FSIEKRQDIISQTFKNEPRVKVSIYDSLTVDFARSVDAGFILRGLRSVSDFEYEHNIA
                     DANRKISGIETVILFTDYEYSYISSTVVRDLIVYGKDISIFLPPNVKI"
     gene            3588..5240
                     /locus_tag="PLD44_02250"
     CDS             3588..5240
                     /locus_tag="PLD44_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOF98191.1"
                     /translation="MKKKKFFLFLLIFCTPFLLVAQQDNQRTFRISKNLNIYNSLLRE
                     LDMFYVDTLNYDKLIKTSIDEMLNTLDPYTIYLPEEETEDLTFMTTGEYAGIGALIMK
                     NGNEIVVSEVYEGMPAQRNGIRPGDIIVEVDGKKTDGLAVNDVSNLLRGTPNSQINIK
                     IKRYGEKKIISKTFLREKIQINPIGYAAEVDDKIGYLLLNEFTENAALGVKSAVNDLI
                     KNHHIESLVLDLRNNGGGLVDEAVQILGYFLPKGTTVVTTKGKNNILDRIYKTPTEPV
                     FPNLKLAILVNRSSASASEILAGAIQDLDRGVIVGERTFGKGLVQNIRNISYGGHLKV
                     TTAKYYIPSGRCIQAIDYSHRNEDGSVGYVPDSLTSEFLTKNGRKVRDGGGIIPDKQT
                     ESKGKLNVAHYIFAQNLYFDFATRYASLHPTIASPKDFKLSEDDFNNFVDYLHEKNFT
                     YSYQTNKYFQQLYQVAKIEGLDSIAQSEFEALKTKLTPNVDADIRENKDEIEALLSLE
                     IIKRYYYQKGQIEFSLRTDDDLKEAIQILKSEETYRKILSAI"
     gene            5356..5952
                     /locus_tag="PLD44_02255"
     CDS             5356..5952
                     /locus_tag="PLD44_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOF98192.1"
                     /translation="MAVITPFIMEKKNIGEKILALCKSRNLSIQDLVDTTGLSEVQIN
                     RVIESETIPSLAPLIKIARALGVRLGTFLDDAEELGPVVHRSGEVHQPASFSSQLSDA
                     NSHLDFYALASRKTNRHMEPFFIDIQPSSTHEPIFSSHEGEEFIFVLEGTAKIDYGNQ
                     TFFLHPGDSIYYDSIINHLVSSADEKPAKILAVVYTPL"
     gene            6056..7705
                     /locus_tag="PLD44_02260"
     CDS             6056..7705
                     /locus_tag="PLD44_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOF98193.1"
                     /translation="MELFEKTIGEWLEYWANETPEREYLVYADRNLRFTWKSFNERVD
                     NMAKGLMAIDVKKGDHVGIFAQNVPDWLTFLYACAKIGAIAVTLNTSCKEHELAYIIE
                     NSDMHTLCMTEGDNGNDFIKTIHSLIPELKTSERGRLKSNRFPVLKNVVFIGQEKHRG
                     MYTTAEILLLGTYQNNEEYFQLKKTINCQDVVNMQYTSGTTGFPKGVMLTHHNITNNG
                     FYTGEHMKFTNDDKLCICVPLFHCFGIVLAVMNCLTHGCTQVMVEKFDALLTLASVHK
                     ERCTALYGVPTMFIAELNHPMFNLFDLTSLRTGIMAGALCPIELMRNVNEKMYMNITS
                     VYGLTETSPGMTQTRVDDSFEVRCTTVGREYEFVEVAVLDPKTGAPCPDGVEGEMCCK
                     GYNVMKGYYKNPEATHQVIDANGFLHSGDLGVRDKNGNYRITGRIKDIIIRGGENISP
                     IEVEDFLYQMPGIKDVQVVAIASPRYGEDVAAFIIKKDGFDIREEDVRDFCKDKIARY
                     KIPRYVFFVDEYPLTGSGKIQKFKLREMALQLCKERGIEIV"
     gene            7764..8543
                     /locus_tag="PLD44_02265"
     CDS             7764..8543
                     /locus_tag="PLD44_02265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOF98194.1"
                     /translation="MKKLWRIYRYFLHRFTTRNTHGFGVHSPFLFHFICFVVEEKNPY
                     YIFSSIENLRDRLLKDRTKIRIEDFGTGKSRTSTIATVVNKSVKSKKYGQLFFRITLH
                     TKAQSVLELGTSLGITTAYLAAASSQLHCISLEGSQTLADLAKKNFQQLGLKNISLIE
                     GNIDENLEKVLESTPSFDLIFIDANHTSQAVIEYFNMCLEKIHTHSIMLVDDIYWSAD
                     MLKAWKYIKQHPKVTATIDLFETGIVFFDPNLYKKHYKVRY"
     gene            8692..9252
                     /locus_tag="PLD44_02270"
     CDS             8692..9252
                     /locus_tag="PLD44_02270"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOF98195.1"
                     /translation="MKLYTKTGDEGETGLIGGTRVPKNHIRLEAYGTVDELNSFIGLL
                     TTFQIDNSDLLFLQSIQNKLFIIGSYLATDFSKYSKTKVSPLLEKDITNIETEIDRLT
                     SEVPPLNNFVLPGGSQASALCHICRTITRRLERRLYDLHEQIPVDKNILIYINRMSDY
                     FFALSRYLLFKNNIQEILWQKNEEND"
     gene            9340..9561
                     /locus_tag="PLD44_02275"
     CDS             9340..9561
                     /locus_tag="PLD44_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOF98196.1"
                     /translation="MYWTLELASKIEDAPWPATKDELIDYAIRSGAPLEVIENLQEIE
                     DEGEIYESIEDIWPDYPSKEDFFFNEEEY"
     gene            9743..10690
                     /locus_tag="PLD44_02280"
     CDS             9743..10690
                     /locus_tag="PLD44_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOF98197.1"
                     /translation="MKKIVLTVVVFMVSSVSIISQTASQWSQYMFNSSSFNPAAVGEK
                     GMIEAVWQHRIQWVGMPNAGQSDIINLNLPVDFLSTTHGVGLKVIDDKVGLFSNTSVY
                     LQYAYKLKTTSGVWSFGTELGLINAGFEGDSVHSVNIGDYHESTDPVVPSTNVQGMGF
                     DIGLGAWYSSPKFEAGISYAHLNQPVISWSDQLDYQEKGVLFITGSYNWTLIDPKMVI
                     KPTTLFKTDFVSYQWDASARMEYDNKYWGGLSYRWGDAVVFFAGINAIAGLNIGYAYD
                     LPVSQIIKASSGSHEFLISYSFEYKLNKAKHKYKSIRIL"
     gene            10749..12116
                     /locus_tag="PLD44_02285"
     CDS             10749..12116
                     /locus_tag="PLD44_02285"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOF98198.1"
                     /translation="MKRNVLISILFGSLLLAGCNKPAGELVGVGNRGNFREANPYGMV
                     FIKGGSFLMGANDQSAIGAINDKSIHVTVDAFWMDQTEITNDEYKQFVNWVRDSIILR
                     SLVMAGRDEFRLKLRNQDELPPETAPLNWKAKIPWKSQDEEIREVLNTFYYEDVNSLS
                     GRQLNPTKFRYRYEWINYDQAALATNKYDVNTGAYPKNAKVRVDTSYVDENGVIHHQT
                     IERPLRTRKDLISTKIVNIYPDTIMWIRDFQYAYNEPKMKMYFSHPGFANYPVVGVTW
                     EQAQAFCQWRTHIYNGTNKISAQDYRLPTEAEWEYAARGGRQMALYPWGGNYVRDKRG
                     CFMANFKPMRGSYNDDTGTTTMKVGSFLPNDFGLYDMAGNVAEWTSSAFNVSSSNIVY
                     DINPNFQFNAKNSDPQVLKRKVVKGGSWKDIAYYLQCGARTYEYQNESRPYIGFRCVR
                     SYNGE"
     gene            12143..13021
                     /gene="gldL"
                     /locus_tag="PLD44_02290"
     CDS             12143..13021
                     /gene="gldL"
                     /locus_tag="PLD44_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HOF98199.1"
                     /translation="MSNNAINSKKQSGFSEWWNSPKGRKIVNAIYSVGAGVVIIGAMF
                     KILHLPGANVVLGTGMICEAIIFSMGALDKPFKEYHWDKIFDFENGTARFQTELIGSA
                     TISASPAHPSINYSETLSEEDVEKLSEGIKNLSATARQLASVSDLTAPTSEFIRNITS
                     ASESVSNFSKDQNNLNSSLRNLSGIYVEIGTEMKKAEENTKDYTGEIEKINKNLSSLN
                     AIYEIHLKNVEEQSTKIGNSNKELENILSNLKKINSFSSASAEESEKYKNETVKLAQQ
                     ISDLNKVYGNMLNALS"
     gene            13084..14619
                     /gene="gldM"
                     /locus_tag="PLD44_02295"
     CDS             13084..14619
                     /gene="gldM"
                     /locus_tag="PLD44_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HOF98200.1"
                     /translation="MSGAKNCPETTRQKMIGMMYLVLTAMLALNVSSEVLEGFTMVDN
                     SLHNTIISASVRNEDLYKQFEYLYNQNPTKVKEWLDKALEVKKQSDSLYNYIQNFKYE
                     ILKMVDNKNADPEARRVINKENYDAPGEYALVKGNGKILKEKIDNYRNFLISLSGNSP
                     AKQQMYATIFSTAPSENGVPWEYALFEMMPVSAVITVLTKYQSDIRMAEAELIQYLKA
                     QTDVSDFRVNKIEALVVPNSRYVFRGSKYSAQIVLSAVDSTKTPEFYVGNTPVKNGIY
                     EVNCNQTGSFTYTGFVQLTGNDGVIRKFPFTSDYIVGEPSATISNEDLNVVYRGIDHK
                     FSISVPGVASENISVRVAGATVQKTAGGKFIIRPTQDGEINVAVYAKVEGKEILMGNS
                     IYRVRYLPDPKAYLQYSDAGGITRLIQDGNLNKRLLRGDNVAIVASYGEDELIQAKFN
                     IISFTMLTIYGSVETTGSRLSNRQLADIDRLEGGDYITFKNIKAVGPDGKVRNLGLVQ
                     VQI"
     gene            14669..15571
                     /gene="gldN"
                     /locus_tag="PLD44_02300"
     CDS             14669..15571
                     /gene="gldN"
                     /locus_tag="PLD44_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HOF98201.1"
                     /translation="MKKIGVLTLFLAFILLPGSIKAQINEIPFFDSKGNVNIETTELD
                     TLADTIAVVHHRRDDIVWSRVVYRVIDMREKQNYQLYFPTRPNKEYRNLFTVMIDAIC
                     DGVNVYKKNPRDIKPEFNELLEGEELSRAFAFEEDNTSNLIQIDPITNQRTFNNYRYI
                     QYVRNQLKYLIQEIIFFDKHTSRMYSKIIGIAPLYALHPDNVESNETMGYFRGSVLCW
                     FAFDELRPYLAKQYVIPNGNDTQRMTFDDFFSQKLYASYLLGDSNMFNRMLLDYVIDP
                     AKVRKEQNRIENELLNFEQDLWEY"
     gene            15774..16424
                     /locus_tag="PLD44_02305"
     CDS             15774..16424
                     /locus_tag="PLD44_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOF98202.1"
                     /translation="MKNIILSDNQYISKAGWLYLISTYSLEATIREAATKKELEKLLV
                     EEQESLVILDYTLFDFENVNELIIWSMRFEKTDWLLFSEELSEEFLQQIIYNTHSFSV
                     LTKNSNKEEINLALKETLSGNRFICNFVSNLLIDCQKHLKNDGKPVLTPTEREILKEI
                     ALGKTTKEIASERFVSVHTIMTHRKNIFRKIKVNNVHEATKYAMRAGIVNLAEYYI"
     gene            16441..17502
                     /gene="mnmA"
                     /locus_tag="PLD44_02310"
     CDS             16441..17502
                     /gene="mnmA"
                     /locus_tag="PLD44_02310"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOF98203.1"
                     /translation="MKIAALLSGGVDSSVVVHLLKDQGYTPSLFYIKIGRDEDELLNC
                     SSEEDIEMASLVAQKYGCKLEIIDLHKDYWETVVAYTISKMKEGLTPNPDVMCNKLIK
                     FGIFEQKVGKEFDKTATGHYATITEKDGKIFLSTAKDPIKDQTDFLAQIDYLQVSKLM
                     FPIGHFMKSEVRSIAQKAGLPSAKRQDSQGICFLGKINYNDFIRRYLGEKPGPIVEWE
                     TGKILGKHKGYWFHTIGQRKGLGLSGGPWYVIKKDIAANIVYVSCGYDVETQYGNEFS
                     MRNFHFITENPWQNQTEEANITFKIRHTPEFTKGKILHTNSGWHVISEKKIQGIAPGQ
                     FGVIYDKDSHICIGSGEIF"
     gene            17568..18794
                     /locus_tag="PLD44_02315"
     CDS             17568..18794
                     /locus_tag="PLD44_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOF98204.1"
                     /translation="MTYQTHTLPNGLRIIYLQKKSEVTFCGITINAGSRDENREEEGC
                     AHFAEHLLFKGTRKKNAREIINRMEDIGGELNAYTSKEETVVYAAAMKKYTRRMIELM
                     ADMTLHSTFPQKELEKEREVILDEIASYHDNPSELIFDDFEELIFKDHPLGHNILGNA
                     KLLKKMNSESVSNFVKRCYQPHQMIVFVVGNLNFEHFVKWCEEYFVSESAENINEKQK
                     RNPPEQYRAEKLKLKKKTHQIHTVIGNRCFDLYHPNRLPMYLLNNILGGPGMNSLLNL
                     SLREKHGLVYTAESNYQPFTDSGWWCVYYACDERNDEKCEQLVLEQLQKIVDRPLPES
                     KLRKYKQQLIGQMTVSRENTENLALSLGKSYLRFGKVEEMEEIKKQLENIQPKDLQYI
                     AQEIFNEEQLSVLKYY"
     gene            complement(18949..19932)
                     /locus_tag="PLD44_02320"
     CDS             complement(18949..19932)
                     /locus_tag="PLD44_02320"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HOF98205.1"
                     /translation="MTNYLFRTPVELAPSQQKITYKDKIMMLGSCFSENIGKQLHEAF
                     FQVDVNPFGVLYNPLSISLSLERLIENKGFSRSDIFECQCLWHSFFHSSSFSDVSPEK
                     TLSNIQARFDESCSFLKNTDFLLLTFGTAWVFEDRKTQTVVSNCHKLPSQHFNRRRLS
                     IEEIVEHYTLLIAQLQQLNPSLHLIFTVSPIRHWKDGAHENNLSKSILLLSIEELQKK
                     FKNVDYFPAYEIQMDELRDYRFYASDMLHPSETAINYIWEKFSDTYFSEKTKQIKQRL
                     EMLREKMNHRPLHPNSPEYVSFLQQIELEKNNILNEYPFLKERIKDVNSRN"
     gene            20418..20912
                     /locus_tag="PLD44_02325"
     CDS             20418..20912
                     /locus_tag="PLD44_02325"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HOF98206.1"
                     /translation="MLNTLNLQNYFAFNNDHILEGFSLWSDENIFSYGIKRYVFMGGA
                     HGLSTQTFLNFDLKTGKTLTEKDLFIPDYKEKLTTLIRERLIEENDEISEYRDLNQTD
                     YWVDSIQPNGNFYISDEGINYLFNPYEIGPYYLGETEVRIPYARLKDILKNGNPLEYL
                     YENR"
     gene            20924..21451
                     /locus_tag="PLD44_02330"
     CDS             20924..21451
                     /locus_tag="PLD44_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOF98207.1"
                     /translation="MNCFFNLAKSRRSIRKFLPQKVEPEKISMITKVALMAPASKRSN
                     PWEFVVVQQPEMLQKLSLCRPKNSQFLAGAPLAIVVLADTEKSNVWMVDAAIASIMMQ
                     LQAHDLGLGSCWIHVHDRQMDENTSSEDYIRGLLQIPTQYAILNILAVGYPNETKNPH
                     DEEKLAYEKVHFEKF"
     gene            21467..21790
                     /locus_tag="PLD44_02335"
     CDS             21467..21790
                     /locus_tag="PLD44_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98208.1"
                     /translation="MASKKKSSNLAWGITLLLFGLLFLLRQLQIVPPEIGVFVFDIKN
                     FPFIAGVIFLLFHPNISIGIVLLAVGVLFRLSEIIAWTKNFSEFILPALLIIAGSILI
                     FSKRK"
     gene            21876..22670
                     /locus_tag="PLD44_02340"
     CDS             21876..22670
                     /locus_tag="PLD44_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009434040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOF98209.1"
                     /translation="MKRVVIVGAGNVATHLGRALQNKNFVVSQIFSRTENSATQLASD
                     LGVPFTTDLQLLDREADIYFYCVKDAVLCDVINKIDIPDALHVHTAGSLPLSIFESHL
                     QYRRYGVFYPLQTFSKTRKVNFDEIPIFVEGNSLEAEEEIRQIAQTLSKRVFSSDSEQ
                     RKKIHLSAVFACNFVNHLFSIAEELVKTAGYSFDILLPLITETVNKLNELSPYDAQTG
                     PAVRNDQNIIRQHLLLLENKPEFSEIYSVLSESIYRMHKKYIQTRE"
     gene            22730..23716
                     /locus_tag="PLD44_02345"
     CDS             22730..23716
                     /locus_tag="PLD44_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HOF98210.1"
                     /translation="MKQYLQLLRLPNLLFIAFVQFAMSEAVILPLLMTYGFDAGMSSS
                     KLLLLILSTILIAAGGYVLNDYFDVKIDTINRPDKMIVGKTIPRASAMRYYQILTALG
                     LLSGFILAFLVKSFSLGFIFLVVPGMLWFYSATYKRQFLTGNIIVAFASALSVLLPAI
                     LELAVLESQYGKLIFETPIPKQIYAWVGGFAVFAFFCTLLREIIKDAEDIAGDREMEC
                     HTLPIVWGIKKTKIIVYCLILLIVSGLLAVDFFLIDFSSSLTTRYLIFGLIIPFVCLG
                     YLIFKAENARDFHQASTLSKFIMLLGVSYSFVFYFLQAQQYHFPIFHIFMVQ"
     gene            23851..24621
                     /gene="yaaA"
                     /locus_tag="PLD44_02350"
     CDS             23851..24621
                     /gene="yaaA"
                     /locus_tag="PLD44_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HOF98211.1"
                     /translation="MLMLLSPAKIQNTKLPKNSYPATIPQFLDDAEYLVEKVRELSLR
                     ELAQLLGVNSQLAELNRDRFYSWHRPFTVDNARQAVLVYNGEVFRGLNAASMTENELT
                     YLQQHLRILSGLYGVLRPLDLIQPYRLEISSRLPTFAGNDLYDFWGKKITDAVSQAFQ
                     FSGQPEILLNLCSSEYIKAIDRDFFDGKIVDIEFLENDHGNFRPIVVYLKKARGLMVR
                     YVAQYRIENVEDLKGFDVEGYWYHPELSNDNKMVFVRG"
     gene            complement(25089..26147)
                     /locus_tag="PLD44_02355"
     CDS             complement(25089..26147)
                     /locus_tag="PLD44_02355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOF98212.1"
                     /translation="MSNLLIFGDNLKGLKYLLEEKELKGQIDLVYIDPPFATGGNFVI
                     SNGRATTISNSKNGDVAYSDNVKGSDFIEFLRQRLEILKDLLSERGSIYLHTDYKIGH
                     YVKVMMDEIFGVENFRNDITRIKCNPKNFSRIGYGNIKDMILFYTKTSNPIWNEPKEK
                     HKEVNIEKLYPKKDKNGRRYTTVPIHAPGETSSGDSSKPFKGLLPPPGRHWRTDIKTL
                     EKWDNEGLIEWSSTGNPRKIIYADESEGKRVQDIWEFKDPQYPDYPTEKNSDMLDLII
                     KTSSNENSIVLDCFCGSGTTLKSAHLNNRNWIGIDQSSLAIDAAKRKLGEISASLFVP
                     KPEYEFVDLMENLLAYNS"
     gene            complement(26152..26934)
                     /locus_tag="PLD44_02360"
     CDS             complement(26152..26934)
                     /locus_tag="PLD44_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019036314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOF98213.1"
                     /translation="MNKWINLSIEYANQKSYLDDLFQVYPTIPEGIREIDVQIWREVE
                     NAFIKRDNYRLIKELLKLDLFPIKDSYIAYLKRDSSAIDRNPRTINRICGRIYELGLN
                     KLYERCSEPKETNRQIGPMFKDWLRNKSLGILPVEIDEFMSGNNDAILDGGDKVLMDF
                     AKEKLGYYHDKGLDFVARFNGKYVIGEAKFLTDFGGHQNAQFNDAISTLEVKKVKAIK
                     VAILDGVLYIKGNNKMYKSITQKFNNYNIMSALVLREFLYQL"
     gene            27288..28199
                     /locus_tag="PLD44_02365"
     CDS             27288..28199
                     /locus_tag="PLD44_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOF98214.1"
                     /translation="MGITQLVDIDLKEIVPFIDWNFFFLAWRLPGKYEGIENLCNCTS
                     CEVGWLQQFPENNRTKAEEALKLFRDAQQMLQDFLHEKSISIHAIFGFFRAVSQGDDL
                     LISFGDQTYILPTLRQQHASSDGFCYSLADFINTENDYMGAFATTVEGAETMAENFER
                     EDDLYRAIMVKTLSDRLAEAAAEWLHYKVRTEFWGYAPDEPLEIKNILKGKYQGIRPA
                     VGYPSLPDQSIIFELQSLLGLEKIGIRLTENGAMFPNASVCGLYFSHPKSRYFMIGKI
                     NDEQLEDYARRRGKTVQEMKKWLAANI"
     gene            28275..28487
                     /locus_tag="PLD44_02370"
     CDS             28275..28487
                     /locus_tag="PLD44_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOF98215.1"
                     /translation="MLEKSLKIKGMSCSHCATRVEKALNSLDGVEAKVDLASNSAKLT
                     LAKELSNEEIKKAVENVGYEVTEISE"
     gene            29005..29994
                     /locus_tag="PLD44_02375"
     CDS             29005..29994
                     /locus_tag="PLD44_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98216.1"
                     /translation="MKLEEAYSWIDPLYQFASLLVYPMYYIYFRLLTVDRKFSFKKHF
                     VYLLPSTILFVLYSIAVFFTPQEEYKAFLFGEEHHSLNHLSYIEIVSFLIRIVFLIQV
                     ILTVIGNFRLIRKYGHKAAQYYSNMEDSSISKVRLLNYTLIITAISSFILGMLGRGFF
                     ASEITYLAIASVVFSSMLFAIGWLGNKQKSLNPAYETDSITETKQKSEELSLIRQEEL
                     LNKIEYFFNDRKIYLNSSLTILDVARLTGTNRTYVSSLINHHFHQNFSAFVNSYRLKE
                     LEEKILFNPEYPHQILAETCGFGSIDSMKRAINLRYKLSLQEFKSEVLLKKVV"
     gene            complement(30761..30991)
                     /locus_tag="PLD44_02380"
     CDS             complement(30761..30991)
                     /locus_tag="PLD44_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98217.1"
                     /translation="MNNTDYIYENFQKGNEIYIMDDIEEVAIRYSYSKDGYKTFAKFK
                     GGREYKIDETSNIVTRADMGGTILTKEQYKKF"
     gene            complement(30988..31200)
                     /locus_tag="PLD44_02385"
     CDS             complement(30988..31200)
                     /locus_tag="PLD44_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98218.1"
                     /translation="MIFAKEKPEDAKKIIGEKRWNEIHKETGLEGGLGHKFYEEYRST
                     SKISKEGRKRRNELIDLSTKYYNKFR"
     gene            complement(31473..32702)
                     /gene="clpX"
                     /locus_tag="PLD44_02390"
     CDS             complement(31473..32702)
                     /gene="clpX"
                     /locus_tag="PLD44_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOF98219.1"
                     /translation="MPKNVRHCNFCGRSEAEVEFMILGQDGVQICNECAIQAAEIVRE
                     SARKSKSSHLHIEKIPKPKEIKQYLDQYVIGQEEAKKYLSVAVYNHYKRLMQKTEEDE
                     VEIEKSNIIMVGPTGTGKTLLARTIAKKLHVPFTIVDATVFTEAGYVGEDVESILTRL
                     LQVADYDVKAAERGIVFIDEIDKIARKSDNPSITRDVSGEGVQQGLLKLLEGSVVNVP
                     PQGGRKHPEQKMIPVNTQNILFICGGAFDGIEKKIAARLNTRVVGYGASEENKLIDRN
                     NLLQYIAPQDLKSFGLIPEIIGRLPILTYLEPLDRDALRRILTEPKNSIIKQYVKLFK
                     MDNIELIFEDEVLDYIVDKAIEFKLGARGLRSITETIMMDSMYEMPSKKSTKWVVTLP
                     YAQSKIEKTNVHRLKIA"
     gene            complement(32713..33411)
                     /gene="clpP"
                     /locus_tag="PLD44_02395"
     CDS             complement(32713..33411)
                     /gene="clpP"
                     /locus_tag="PLD44_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOF98220.1"
                     /translation="MNKKNIRSDFRNYATRHLGMNGLAIDQYAGFINDSYISPTILEE
                     RQLNVTQMDVFSRLMMDRIIFLGTEIDDYASNVIEAQLLYLDSAEPGKDISLYLNTPG
                     GSVYAGLGIYDTMQYISSDVSTICTGMAASMGAILLVAGTKGKRFALKHSRIMIHQPM
                     GGAQGQASDIEITAREIQKMKQELYNIIAEHSGNPYEKIEKDADRDYWMTSQEALEYG
                     MIDKVLINERKSKK"
     gene            complement(33529..>34914)
                     /gene="tig"
                     /locus_tag="PLD44_02400"
     CDS             complement(33529..>34914)
                     /gene="tig"
                     /locus_tag="PLD44_02400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOF98221.1"
                     /translation="KNYKYQKTNSMNIVKKDLDQNNAVVTLHVEKADYAENVEKNLRD
                     LRKKANIPGFRPGMVPMSLLKKMYGKSVMADEINKLIAEKLYDYIRENDLNILGDPLP
                     NETEQPEIDFNTQEEFDFVFDLGLAPDFDIELSKEIKINYYNIAVTDQMIEDRIKSYS
                     QRFGRYEEVETVEENDMLRGEVIELENGEEKQNGLQVSEAILTPAYIADPEQKALFLN
                     AKKGDTVIFNPAKAFGNETELASFLKISKEQAKEITSDFSFKINDITRYFDSEINQEL
                     FDKVFGENAVTNEQEFREKVKETIATNLKENSDYRFGIDAKKTLVEKFKNLSFPDAFL
                     KRWLLASNKNLTNEQLESDYPKMIEDLTWQLIKEKLIKKFDIKVNEVEVENYAKEFAR
                     AQLAQYGISGADEELISNYAKSLLEKEDTLRNFIERAGDNKVIVTIKDSVEVEVKEIS
                     PEEFDKLFEEN"
BASE COUNT        11136 a         6298 c         6359 g        11121 t
ORIGIN      
        1 tgtattctat gttactgctc aaaaattggt gttatttctc cgtaattatt tccattatac
       61 tcttaaaaca acttaccatg caaggataac gtaacgttaa agtttagtta aaaacagact
      121 gatattggtt agtttggcaa tcaaatacag tcgattaaaa ttctaacata ttggccttag
      181 tacatgacaa aaaaattaaa aacatttata ttttttattt tcagatattt aataacgggg
      241 ctttgcctta aaccccactt acttttttgt cttgaaacaa ataagtaagc aaaaaaattt
      301 acttagttga agcagctacg ctcaacataa atcaaataaa tttgattttg ttttcgctta
      361 aacgctgctt tcaagactgt gtccacttca ctcgaaaaac ttacgctcga aaagctgaaa
      421 tcgcccaaac tcattccgac gaaatacgtc ggaactcaaa caaggacgat ttttaaacgc
      481 ttttctcact tgtttttcgg ctcaccggac aaagtcaacc ccaagagctt ggcttatttg
      541 ttctgtttta tttacaaata tataaaacaa atgtctcaat ttttgtcatg tactaaaact
      601 attttcatat gagaacagtc agtagccttg acattcaaaa aattacatga aaatcgaggc
      661 atttgcaaac gtgtgaaaat aaaaatcaag ttcacaagcc tttagaaatt taccgttcca
      721 tacttctgag cacaaatatt accttaactg catttaactt caacaaagat gttaaatata
      781 tggaaaccat ctttttggct cgaatttaaa atacaaattg ttgacttgat aaacgctacg
      841 ttttaccaag tcaacaattc tttaatacaa aaatgggctt tttaaggctc tactaattaa
      901 aacaatttcc aacccaacga aatgacaatg gtattggctt gaaaatcatc taccgttaca
      961 tcctgaattt tagtttgata catgtcttta atcacattga atcgggcatc caacgtaaat
     1021 tttaaaacat ctactcctaa accggcttcc aatcccagtt gagcattttt tacatccttc
     1081 tcaagttcgg cggcagttat attcttcaaa ttttcaaatt tgagtgaaga gcctgcatta
     1141 aaagttaatt ttggtccggc aaaaacccga acattgacca ttttaatcaa atcgatgact
     1201 ttgtatccaa ccagcaaagg aacttctatg ttgctgatgt ttacaacctt gtcgtaactt
     1261 gcgctttgat tctgcgtatc ctgcagggta acggtatatt ccttcttacc catagtgtag
     1321 agcaattcgg gttgaaaata aattttccca aaatttaatc gtccaaatac tccaagctga
     1381 aaattgtttt ttacgtctgc tcgaatggaa tccacattgt aattaccgga agttacagaa
     1441 ctcaattcat tcaatccgac agaggaatta taaccggctt tcacgccaaa atcaatttgg
     1501 ctgaataccg aaaccgaaaa gaaaagcatt acggcagaat aaataaatct tttcataatt
     1561 tttttatttt aagagttaat aacttaaaaa caatatatat aacgaaaata aattgaaatt
     1621 cttatttgcc aatattttaa tttccctcac caaactgccc aaatgggttg atgaattttc
     1681 agatgctgcg gattaaactt taaatctgcc gtatcgggaa tttgaattct gtacgcttta
     1741 cctttggggt tggtcagctt tcggtctctc aaccacatat taaattcttt cagttgaaaa
     1801 taggtcaaat gatattgttt cgcccattcg gtccaatctt caacggaagt acttacaaca
     1861 atttcttttg tgggaatata cggataaaaa tcatcctttt tcaattgata accaaaaagt
     1921 tttggatttt caaaaaactg tttcatggcc agaattctga aaatatatcg cgaactttcg
     1981 gttgaaagca acatatcgta ggcatcttct acctgctgtt tctcctgttc acccgaaatt
     2041 tttgccatac ctgcattata ggaagctgcc accgtaaccc aactgtgata ttttgcatag
     2101 gcactcttca aaaattggca ggccgcaaca gtagcctttt ccaagtaata acgctcatca
     2161 atttgatcgt tgacttccaa cccgtactgt tcggcggtct tcggcattaa ttgccaaata
     2221 ccggcagcct gagccggaga aaaagcacgc agatcgagat tgctctctac aacagccaaa
     2281 tatttaaaat catccggaat accgttgttt tttaaaatag gttcgattac ggggaaaaac
     2341 cgattggcac gcttcagcaa taaaatgctg gtggaatggt tataagctat caccgtctgt
     2401 tctctatcgt aacgttcacg catatcgatt ctttccaaag aaacagtttc attggcaaaa
     2461 acaacctgtt tgggtattgg cagtgccgta accggatcaa tagcccacaa aagggaatca
     2521 ataaaaatat taaaaccaaa aaacaaaaca aaaaattttc gtatcatcac aaaatctttc
     2581 aattaattgc agcaaagtta ataaaccatt ttaaaactga aaataaaatg caaaaaaatg
     2641 cacttcgatg cagaatttag ttggccataa aagaattgaa aaaataaaac ctgaaaccaa
     2701 tgaacgacgt ctgaaaattc tgtaagggga actcgagaaa aaccaatttt atcagcgata
     2761 tacagaaatt gaaaattatc atgtaaattt gcacataaat aaatttaatc aaaaatgaat
     2821 ggacatccct tgttgcaaaa aatggtttcc ttagtagcaa aaaagtgcga aatgagggca
     2881 actttccttc aaccaagttc ttgtcgattc aaaataattt agatcatgaa acgagccatt
     2941 ttcccgggaa cgtttgaccc atttaccatt ggacactaca gcattgtcaa gcgaggattg
     3001 aagcttttcg atgagataat tattggcatt ggagttaatc aggcaaaaaa aaccttattc
     3061 tccatcgaaa agaggcagga cataataagt caaacattta aaaatgagcc aagagtgaag
     3121 gtttctattt acgacagcct cacagtggat tttgcccgtt ctgttgatgc aggtttcata
     3181 cttcgaggat tgcgctctgt tagcgatttt gaatatgaac acaatattgc cgacgccaac
     3241 cgaaaaattt ccggaattga gacagtcatt ctgtttaccg attacgaata ttcgtatatt
     3301 tcttcaacgg tggtgcgaga tttaattgtt tacggaaaag atatttcgat tttcctgccg
     3361 cctaatgtaa aaatttaata aattcatttt cgatcattta tattcattca aacgaaaaga
     3421 aataaaaata atttccctaa gaattttgtt ttcctacaaa atccattcaa gaaaaaaatt
     3481 aatgacaaac gcatgaaacc cacatgtatt gcatacacaa acacgaataa atataatatt
     3541 cagacatgtg ggttccatca gacctttaaa aaataattta tattctgatg aaaaagaaaa
     3601 aattcttcct gttcctccta attttttgca ctccattttt acttgtggct cagcaagata
     3661 atcaacgtac atttcgtatc agcaaaaatc tgaatattta caattcattg ctgcgtgagt
     3721 tggacatgtt ttatgtcgat accctgaatt acgacaaact gataaaaact tctatcgacg
     3781 aaatgttgaa cacgctggat ccttatacaa tttatctgcc cgaagaagaa accgaagacc
     3841 ttacctttat gacgacaggc gaatatgccg gtattggtgc acttatcatg aaaaacggaa
     3901 atgaaattgt tgtttccgaa gtttatgaag gcatgccggc gcaacgtaac ggtattcgac
     3961 ccggggacat cattgtggaa gtagatggga aaaaaactga tggccttgcc gtcaacgacg
     4021 ttagcaattt attgagagga actcccaata gccaaatcaa cataaaaatc aaacggtacg
     4081 gagagaaaaa aatcatcagc aaaacttttc tgcgtgaaaa gattcaaatc aatcccattg
     4141 gctatgcggc agaagttgat gacaaaatag gctatctgct gctcaacgaa ttcaccgaaa
     4201 acgcagcgtt gggagtaaaa tcagctgtaa acgatttgat aaaaaatcat catattgaat
     4261 ctcttgtact cgatttgcgc aacaacggag gcggattggt ggacgaagcc gttcaaattt
     4321 tgggatattt tctgcccaaa ggcacaacag tggttaccac caaaggtaaa aacaatatac
     4381 tcgacagaat ttataaaact cccaccgaac ctgtttttcc caacctcaag ctggcaatct
     4441 tggtcaaccg atcttcagcc tctgcctccg aaattcttgc cggagccatt caggatctcg
     4501 atcgcggtgt aatagtcgga gaacgtacat ttggcaaagg attggttcaa aacattcgca
     4561 acatcagtta cggcggacat ttgaaagtta cgacagcaaa atattacatt ccgagtggca
     4621 gatgcataca agccatcgac tacagtcatc gcaatgaaga tggaagtgtg ggatacgtac
     4681 ccgacagcct gacttccgaa tttctgacaa aaaacggtcg aaaagttcgc gacggtggcg
     4741 gaattatccc cgacaaacag accgaaagta aaggaaaatt gaacgttgcc cactatattt
     4801 ttgcacaaaa cctgtatttt gattttgcga cccgctacgc cagtttacat cctactattg
     4861 cgtctccaaa ggattttaaa ctttctgaag acgactttaa caattttgtg gattacctgc
     4921 acgaaaagaa ttttacttac tcatatcaga ccaataaata ttttcagcaa ctctatcagg
     4981 ttgccaaaat agaaggctta gattcaatag cacaatctga gtttgaagca ttaaaaacca
     5041 aattaacacc caatgtagat gccgatattc gggaaaacaa ggatgaaatt gaggcattgc
     5101 tcagtctgga aataattaaa agatactatt atcaaaaagg acaaattgag ttttcacttc
     5161 gaactgatga cgatttgaaa gaagcaattc aaattttaaa gtccgaagaa acctatcgaa
     5221 aaattttgtc ggccatttaa aaaacagttg atattttata agttaagtaa aggtttttga
     5281 ttggaaataa aatatttttc cttcaaaaac cttcatttta taaattttta tcgtactttt
     5341 gttcccgttt taaatatggc agttataaca ccttttatca tggagaaaaa aaatatcgga
     5401 gaaaaaattc ttgccctttg taagagtaga aatctaagca ttcaagattt ggttgacacc
     5461 acaggtttat ctgaagttca aattaataga gtgatcgaaa gcgaaactat tccttcgctg
     5521 gccccactta taaaaattgc acgcgcattg ggcgttcggt tgggaacttt tcttgatgac
     5581 gcagaagaat tgggacctgt tgtccatcgt tccggcgaag ttcatcaacc ggcttctttt
     5641 tccagtcagc tttcggatgc caattctcat ctcgattttt atgcgttggc atcgcgcaaa
     5701 accaaccgac acatggaacc cttcttcatc gatattcaac cttcatcgac acacgaaccc
     5761 attttttctt cccacgaagg agaagaattt atttttgttc tggaagggac agcaaaaata
     5821 gattatggca accaaacttt tttcctccat cccggcgaca gcatttatta cgactctatt
     5881 atcaaccatc tggtcagctc ggctgatgaa aaaccggcca aaattctggc tgtcgtttac
     5941 acacctcttt aatcttttca ccttgttgaa ttattgagaa ttttattgct gttttttccg
     6001 gtctgaaaaa attttatttc catcaagcac cataaagctt tatacaaatc ttatcatgga
     6061 attatttgaa aaaaccatag gcgaatggct cgaatattgg gcaaatgaaa ctcccgagcg
     6121 tgaatacctt gtttatgccg atcgaaattt gcgtttcaca tggaaaagtt ttaacgaacg
     6181 tgtggacaat atggccaaag gtttaatggc catcgatgta aaaaaagggg atcatgtcgg
     6241 tattttcgct caaaacgtgc ccgactggtt gacttttctt tatgcctgcg ccaaaattgg
     6301 agcaatagcc gtaacactca ataccagttg taaagagcac gaacttgcct atattattga
     6361 gaattcggac atgcatacat tgtgtatgac agaaggcgac aacggaaatg actttattaa
     6421 aaccatccat tcgttgattc ctgagctaaa aacttcggaa cgcggaagac tgaaatcaaa
     6481 ccgctttcct gttttgaaaa atgttgtctt tatcggacag gaaaaacaca gaggcatgta
     6541 caccactgca gaaattctgc ttcttggaac ttatcagaat aatgaagaat attttcaact
     6601 gaaaaaaacg atcaattgcc aagatgtggt caatatgcaa tatacttccg gaactaccgg
     6661 tttcccaaag ggagtaatgc ttacccatca caacataacc aacaacgggt tttacacagg
     6721 agaacacatg aaatttacca atgacgacaa actgtgtatc tgtgttcctc ttttccactg
     6781 ttttggcatt gtattggcag taatgaattg tctgacacat ggttgtaccc aagtaatggt
     6841 tgaaaaattt gatgcacttt taacgctggc ttctgttcat aaagaacgtt gcacggcact
     6901 ttacggagtc ccgactatgt ttatcgccga gctgaatcat cccatgttta atttgtttga
     6961 cctcacctca ttgcgtaccg gcatcatggc aggtgcactc tgtcccatag aactgatgcg
     7021 caacgtaaat gaaaaaatgt atatgaacat caccagcgtt tacggactca ccgagacatc
     7081 tcccggtatg acgcaaacac gagttgatga ttcgtttgaa gttcgctgta ccaccgttgg
     7141 aagagaatat gaatttgttg aagtggcagt tctcgatcca aaaacaggcg ctccctgccc
     7201 cgatggtgta gaaggcgaaa tgtgttgcaa gggctacaac gtaatgaaag gttactacaa
     7261 gaatccggaa gccacccatc aggtaattga tgccaatgga tttttgcatt caggcgattt
     7321 gggagtcagg gacaagaacg gaaattatcg aattacggga cgaattaagg atattattat
     7381 tcgaggtggc gaaaatattt cacccatcga agttgaagat tttctttatc aaatgcccgg
     7441 aataaaggac gttcaggtcg tggccattgc ttccccgcgt tacggagaag atgttgcagc
     7501 ttttattatc aaaaaggatg gatttgacat tcgggaagaa gatgtcagag atttttgcaa
     7561 agataaaatt gcccgttaca aaattccgcg ttatgtattt tttgttgatg aatatccatt
     7621 gaccggcagc ggaaaaatac aaaaattcaa actgcgcgaa atggctttac aactctgcaa
     7681 ggaaagagga attgaaattg tttgaacgta acggtgaaaa taaaaatgta cctttgtaag
     7741 gtttcttgat attttttttc gcaatgaaaa aactatggcg tatttacaga tattttttac
     7801 atcgttttac tacaagaaac acgcatggtt ttggcgtaca ttctccattc cttttccatt
     7861 ttatttgttt tgttgttgaa gaaaaaaatc cctattatat tttttcttcc atcgaaaatt
     7921 tgagagatcg cctcctcaaa gacagaacaa aaatacgaat tgaagatttc ggtacgggta
     7981 aaagcaggac ttcaaccatt gcgacggtag tcaacaaatc ggtaaaatca aaaaaatacg
     8041 gtcaactttt ttttcggata acactccaca ccaaagcgca atctgttctt gaactgggaa
     8101 cttcactcgg tattacaacg gcatatctgg ctgcagcttc ttctcagctt cattgcataa
     8161 gtctggaagg gtcccaaaca ttggccgact tggcaaaaaa aaatttccaa caattgggac
     8221 tcaaaaatat ttccttaatc gaaggaaata ttgatgaaaa cttggagaaa gttcttgaaa
     8281 gtactccgtc attcgactta attttcattg atgccaatca tacgtcgcaa gccgtaatag
     8341 aatacttcaa catgtgcctt gaaaaaattc acactcattc cataatgctg gttgacgata
     8401 tttattggtc tgccgatatg ctgaaagcct ggaaatacat caaacaacat ccaaaagtaa
     8461 cagctaccat cgatttgttt gagacaggaa tcgtattttt cgaccccaat ctatacaaaa
     8521 aacactataa agtgcgttac taaattgcta ttgcacaaaa catattaata tattcgttca
     8581 acaaaaaatc acgaaaaatt gttatgtggt cagattgcca ttgaaaaaca aatcaacaaa
     8641 ttaaaaaaca actgattttc aaaacaatag aaaaaaaaga aatcaaacgg tatgaaacta
     8701 tatacaaaaa caggagatga aggtgaaacg ggattaattg gagggacgag agttccaaaa
     8761 aatcacattc ggctggaagc ttatggtacg gtagatgagc taaattcatt tattggcttg
     8821 ttgaccactt ttcaaataga taattcggat ttactttttc tgcaatccat tcaaaataaa
     8881 ctttttatca ttggctctta tttggcgacc gatttttcta aatattctaa aacaaaagtt
     8941 tcgccactgt tagaaaaaga tataacaaac atagaaacgg aaatagaccg attgacgtcg
     9001 gaagttccgc cattgaataa ttttgttcta cccggtggca gtcaagcctc agccttgtgc
     9061 catatctgca gaaccattac acgtcgtttg gaacgacgtt tgtacgactt acatgaacaa
     9121 atacctgtcg acaaaaatat actgatttat atcaatcgca tgtccgatta tttctttgcc
     9181 ttgtcgcgat atttgttgtt taaaaacaat attcaggaaa tattatggca aaaaaatgag
     9241 gaaaacgatt aatttgtaac ttttttaatt atttttgcgc aaaatttaga ggaaaaataa
     9301 ttattcaatc aaaaaaattg aacctaaata tttagctata tgtattggac gttagaatta
     9361 gcttcaaaaa tagaagatgc accctggccg gcaactaagg atgaattgat agattatgct
     9421 atccgctccg gtgcgccatt ggaagtaatc gaaaacttgc aggaaattga agatgaaggt
     9481 gaaatctatg aatccatcga ggacatttgg cccgattatc cgagcaaaga agactttttc
     9541 ttcaatgaag aagaatatta atctttcaaa attctttttt tacaaaactt tacttatttt
     9601 tgttgagcta aaaataagga ttaggagtaa tggatacggt gaaagtgcaa tttttttgtt
     9661 aaattgaagt tatttataaa gttgacaagc ttttttgatc gaaaaaaagg ttgaataaaa
     9721 acttaataat aagaatcatc tcatgaaaaa aatagtactc actgttgttg tatttatggt
     9781 ttcatcagtg agtattattt ctcaaactgc atctcagtgg agccagtaca tgtttaattc
     9841 ttcttccttc aatccggcag cggtcggcga aaaaggaatg attgaagccg tttggcaaca
     9901 tcgtattcag tgggttggaa tgcctaatgc gggtcaatcg gatattatta acctcaactt
     9961 gcctgttgat tttttgtcca ccactcatgg tgtcggttta aaagtcatag atgataaggt
    10021 tggcttattt tctaacactt cggtatatct gcaatatgca tataaactaa aaacaacttc
    10081 aggagtttgg agttttggaa cagaattggg tttgatcaat gcagggtttg aaggagacag
    10141 cgttcattca gtcaacatag gtgattacca cgaatcgacc gatcctgtgg taccatcaac
    10201 caatgttcaa gggatgggtt tcgatattgg gttgggagct tggtattctt ctccaaaatt
    10261 tgaggcagga atttcctacg cacatctcaa tcagcctgtc attagttgga gcgaccagct
    10321 tgattatcag gagaaaggtg ttctttttat cacaggtagt tacaattgga ctttgattga
    10381 tccgaaaatg gttataaaac cgacaacgct ttttaaaacc gattttgttt cttatcagtg
    10441 ggatgcttct gcacgtatgg aatatgacaa taaatactgg ggaggacttt cctaccgatg
    10501 gggcgatgcg gtggtatttt ttgctggaat caatgccatt gctggtttaa atattggtta
    10561 tgcttatgat ttaccggttt cgcaaattat aaaggcaagt tccggttctc atgaattttt
    10621 gataagttat agttttgaat ataaattaaa taaagcaaaa cacaagtaca aaagtattcg
    10681 tattttgtga caaaagtatt ttttcagaaa aaagattaaa aaaacaatca ctttattgtt
    10741 atacaagaat gaaaagaaat gtgctaatta gtatcctttt cggatcattg cttctggccg
    10801 gatgcaacaa gcctgccgga gaattggttg gagttgggaa cagaggaaat tttcgcgaag
    10861 ccaatcccta tggtatggtt ttcataaaag gaggttcgtt tttgatggga gccaatgacc
    10921 aatcagcaat tggagctatc aatgacaagt cgatacatgt aacggtcgac gccttttgga
    10981 tggatcagac cgaaattact aatgacgagt acaaacaatt tgtgaattgg gtgcgtgatt
    11041 ccatcatttt acggtctctt gtaatggctg gccgagacga atttcgcctg aagttgagga
    11101 atcaggatga gcttccacct gaaacggcac ctttaaattg gaaagctaaa attccatgga
    11161 aatcccaaga tgaagaaatt agagaggtgc tcaatacatt ttattatgag gacgtaaact
    11221 cactttcagg cagacagttg aatccaacca aattccgtta tcgttatgaa tggattaatt
    11281 atgatcaagc tgctttggct accaataaat atgatgtaaa cacgggagcc tatccaaaaa
    11341 atgcaaaagt tagagttgac acctcttatg ttgacgaaaa cggagtgatt catcatcaaa
    11401 ctatcgaacg tcccttgaga actcgtaaag atttgatttc cacgaaaata gtaaatatat
    11461 atcccgatac tatcatgtgg attagagatt ttcagtatgc ctacaacgag ccaaaaatga
    11521 aaatgtactt ttcccatccc ggctttgcca attatcctgt ggtaggggta acatgggaac
    11581 aggcacaggc attttgtcaa tggagaacgc atatttacaa cggtacaaac aaaatcagtg
    11641 ctcaggacta tcgtttgcct accgaagccg aatgggaata tgcagctcgg ggtggccgtc
    11701 aaatggcttt gtatccttgg ggtggcaatt atgttagaga caaaagagga tgttttatgg
    11761 ctaatttcaa acccatgcgt ggaagttata acgatgatac cggaacaaca accatgaaag
    11821 ttggttcatt tcttccgaac gattttggac tgtacgatat ggcaggaaat gtagcagaat
    11881 ggacttcatc agctttcaat gtctccagca gcaacattgt ttacgatatt aacccgaatt
    11941 ttcagttcaa tgcaaaaaac agcgatcctc aagtactgaa acgcaaagtg gtaaaaggcg
    12001 gatcatggaa agacattgcc tactacctgc aatgcggtgc acgtacctat gaatatcaga
    12061 acgaaagcag accttatatt ggatttagat gtgtacgatc atataatggc gaataaaagt
    12121 gtaaacttta ataattacaa acatgtcaaa taatgctata aattcaaaaa aacaatcagg
    12181 attttccgaa tggtggaatt ctcccaaagg gagaaaaatt gttaatgcca tatacagcgt
    12241 tggagccgga gtagttatca tcggtgcaat gtttaaaata ctgcacttgc cgggtgcaaa
    12301 cgtggtgcta ggtaccggca tgatttgtga ggcaattatt ttttcaatgg gagcattaga
    12361 taaacctttt aaagaatacc attgggataa gattttcgat tttgaaaatg gaacagcccg
    12421 atttcaaact gaactaatag gaagtgcgac aatatccgct tctccggctc atccatctat
    12481 aaattattct gaaacgcttt ctgaagaaga tgtagaaaaa ttatcggaag gaataaaaaa
    12541 tctttctgcc acagcacgac aactagcatc tgtttctgat ttaactgccc caacttcgga
    12601 gtttatccgc aacataactt ctgcttctga atctgtaagc aatttttcga aagaccaaaa
    12661 taacctgaac tcttctcttc gtaatttatc agggatttat gtggaaattg gtactgaaat
    12721 gaaaaaagca gaagagaaca caaaagatta cactggggaa attgagaaaa tcaacaagaa
    12781 tttaagttca ttgaatgcca tttacgaaat tcatttgaaa aacgtggaag aacaatcaac
    12841 taaaatagga aattccaaca aggaattgga aaatattcta tcgaacttga aaaaaattaa
    12901 ttcattttcc tctgcaagtg ctgaagaatc agaaaagtat aaaaacgaaa ctgttaaact
    12961 ggcacaacaa atttctgatc tcaacaaagt ttatggcaat atgttgaatg cattaagctg
    13021 ataacaaaga gatttttatt ttttaaattt tcttaaaata agacattaga aataaaataa
    13081 aagatgagtg gagcaaaaaa ttgtcccgag accacccgac aaaaaatgat cgggatgatg
    13141 tatttggtgc tgacagccat gctggcattg aatgtatcaa gtgaagtgct ggaagggttt
    13201 actatggttg acaacagctt gcataacact atcatttcag cttcggtaag gaatgaagat
    13261 ttatacaaac aattcgaata tttatacaat cagaatccca caaaagtaaa agaatggtta
    13321 gataaagctt tggaggttaa aaaacaatcg gattctcttt acaactatat tcaaaatttc
    13381 aaatatgaaa tattgaaaat ggttgataat aaaaatgctg atcctgaagc gcgtagggtg
    13441 attaataaag aaaattacga tgcacccggt gaatatgcat tggtaaaagg taatgggaaa
    13501 attcttaagg aaaaaattga taattataga aattttttga tctcattatc tggcaatagt
    13561 ccggcaaaac agcaaatgta tgctactata ttttcaaccg ctccatcaga aaacggagta
    13621 ccttgggaat atgctttgtt tgaaatgatg cccgtttcgg cggttataac cgtactaaca
    13681 aaatatcaaa gcgatattcg aatggcagaa gccgaattaa tccagtacct caaagcacaa
    13741 actgacgttt ccgatttcag ggttaataaa attgaagcct tagttgttcc aaattctcgt
    13801 tacgtattta gaggaagcaa atattcggct caaattgttc tttcagccgt tgattccact
    13861 aaaacgcccg agttctacgt aggtaatacc cctgtaaaaa atggaattta tgaagttaat
    13921 tgcaatcaga caggcagctt tacttatact ggtttcgttc agttaacagg gaacgatggt
    13981 gtgataagaa agtttccctt tacaagtgat tatattgtcg gtgagccttc ggcaaccatt
    14041 tccaatgaag atctgaatgt tgtttatcgc ggcatcgacc ataaattcag tatctctgtt
    14101 ccgggagtag cctctgaaaa tatttcagta agggtggcgg gagcaaccgt tcaaaaaact
    14161 gctggaggta aattcattat tcgtcccaca caggatgggg aaatcaatgt tgctgtgtat
    14221 gccaaagttg aaggaaaaga aattttaatg ggtaacagta tttatagagt gagatatttg
    14281 cctgatccaa aagcttattt acaatattct gatgccggtg gaattacaag attaatacag
    14341 gatggaaatc ttaataagcg tttattgaga ggcgacaatg tagccattgt ggcaagttat
    14401 ggtgaagacg aactcattca ggcaaaattc aatattattt cttttacaat gctcactata
    14461 tatggttcag ttgaaaccac cggatcaagg ttaagtaata gacaattggc tgatattgat
    14521 cgtttggagg gtggagatta tattacattt aaaaatatta aagccgtcgg tcctgatggc
    14581 aaagtaagaa atcttggatt agttcaagta caaatttagt tttttaaaat tttttgcaaa
    14641 aataagcaaa ccaaatagaa tgttcagtat gaaaaaaatt ggtgttctaa ctttattctt
    14701 ggcttttatt ctactaccgg gttccataaa agctcaaatt aatgaaattc ctttttttga
    14761 tagcaaagga aatgttaaca ttgaaactac cgagctcgat acattggcgg acaccattgc
    14821 agttgttcat catcgtagag acgatattgt ttggtcgaga gtggtatata gggttataga
    14881 tatgcgagaa aaacaaaact atcaactata ttttccaact cgacccaata aggaatatcg
    14941 caatcttttt acggtaatga tcgacgcaat ttgtgatgga gttaacgtgt ataaaaaaaa
    15001 tccgcgcgat atcaaacctg aattcaacga attgttggaa ggggaagaat tatccagggc
    15061 ttttgctttt gaagaagaca ataccagcaa tcttattcaa atagatccca taaccaatca
    15121 gcgaaccttc aacaattacc gttatattca gtacgtgcgc aatcagttga aatatctgat
    15181 tcaggaaatt attttcttcg ataagcatac ctccagaatg tattccaaaa tcattggtat
    15241 tgcccctctt tatgctttac atcccgataa tgtcgaatct aatgagacaa tgggatattt
    15301 cagaggttcc gttctctgtt ggtttgcatt tgacgaactg cgtccctatc tggccaaaca
    15361 atatgttatc ccaaatggga acgacaccca acgcatgact tttgacgact ttttctctca
    15421 aaaactttat gcaagttact tgctgggcga cagcaacatg tttaacagaa tgctgctcga
    15481 ttatgttatt gaccctgcca aagttagaaa agaacaaaac aggatagaaa acgaattgct
    15541 gaactttgaa caggatttgt gggaatattg aaaaaagctt tcaaatatta ttcaaatatt
    15601 caaaaagacg gctgttgagt cgtctttttt tgcttaacgt atgaaactac attgcaaaaa
    15661 tttttttgga agtatatttt tatcgaacaa gtgaacaaaa ttattcttaa acgattataa
    15721 tcaaaaaaac acaaacgtcg taaagatttt ttaaaaaaag ttattcataa aaaatgaaga
    15781 atatcatact ttccgataat caatatattt ctaaagccgg gtggctttat ctgatctcaa
    15841 cctattctct ggaggcaacc attagggaag cagccacaaa aaaagaactg gaaaaactcc
    15901 ttgttgaaga gcaggaatca ttggtaatat tggattatac actttttgat tttgaaaacg
    15961 tcaatgaatt gataatatgg agcatgcgtt ttgaaaaaac cgactggtta ttattttccg
    16021 aagaattaag cgaagaattt cttcaacaaa ttatttacaa cacccactcc ttcagtgtac
    16081 tcacaaaaaa cagcaataaa gaagaaataa atctggcatt aaaagaaaca ttgtccggaa
    16141 acaggtttat ttgtaacttt gtatccaatt tgctgattga ctgccaaaaa catctgaaaa
    16201 atgatggcaa accggtttta acacctactg agcgtgaaat tctgaaagaa attgcactgg
    16261 gaaaaaccac gaaggaaata gcatccgagc gttttgtgag tgtacacacc attatgacac
    16321 acagaaaaaa tattttccgc aaaataaaag tcaataacgt acacgaagcc acaaaatatg
    16381 caatgcgggc tggaattgtc aatctggctg aatattacat ctaaaatcag gctaaaaaaa
    16441 atgaaaatag cggctttact ttccggaggg gttgacagtt cggtagtagt tcatttgctg
    16501 aaggatcagg gttacactcc ttcactgttt tatattaaaa tcggaagaga cgaggacgaa
    16561 ctgctgaatt gctcttcaga agaggatata gaaatggctt cactcgtagc acagaaatat
    16621 ggttgcaagc tcgaaattat cgacctgcac aaggattatt gggaaactgt tgttgcctac
    16681 accataagca agatgaagga aggattgacc ccaaatcctg acgtgatgtg caacaaacta
    16741 ataaaatttg gcatttttga acaaaaagtt ggaaaagaat tcgataaaac agccaccgga
    16801 cattatgcca ccatcaccga aaaagatgga aaaatttttc tttccactgc aaaagatccg
    16861 ataaaagacc aaacggattt tcttgcacaa atcgattatc tgcaggtttc aaaactgatg
    16921 tttccgattg gacacttcat gaaaagtgaa gttagaagca ttgcacaaaa agccggtctg
    16981 ccaagtgcca aaagacagga cagtcaggga atctgttttc tgggaaaaat caattacaac
    17041 gattttattc gtcgctactt gggagaaaaa ccaggtccta ttgtggaatg ggaaacggga
    17101 aaaattcttg ggaagcacaa aggatattgg tttcatacca ttggacaacg caaaggtctt
    17161 ggactatcgg gagggccgtg gtatgtgata aaaaaagata ttgctgccaa tatcgtttac
    17221 gtttcctgcg gatatgatgt agaaacacaa tatggaaatg aattttcgat gcgcaatttt
    17281 catttcatta ccgaaaatcc gtggcaaaac caaacggaag aagcaaatat aactttcaaa
    17341 atcagacata cacccgaatt tacgaaagga aaaattttgc ataccaacag cggctggcat
    17401 gtgatttccg aaaaaaaaat tcagggcatt gctccgggac aattcggcgt gatttacgac
    17461 aaggattccc acatttgcat cggcagtggt gaaatattct gaaaaaacat cccgtctttt
    17521 ttcacacaaa tgtaaccatc aattatttca acaaacaaat tctgcagatg acttatcaga
    17581 cccatacgct tcctaatggt ttacgaatca tatatctcca aaagaaaagt gaagttacct
    17641 tttgcggcat aacaataaat gccggttcac gcgacgaaaa tcgggaagaa gaaggatgcg
    17701 cgcattttgc cgaacatttg ttgtttaaag gtacacgaaa aaaaaatgca cgtgaaatca
    17761 tcaaccgaat ggaagacatc ggaggagaac tgaatgccta tacatccaaa gaagaaacgg
    17821 tggtttatgc agcggccatg aaaaaataca cccgtcgcat gattgaattg atggccgaca
    17881 tgaccctaca ttccaccttt cctcaaaaag aactcgaaaa agagcgggaa gtaatcttgg
    17941 acgaaatagc gtcctatcat gataatccgt cggaacttat ttttgacgat ttcgaagaat
    18001 tgatttttaa agatcatcct ctggggcaca atatattggg aaacgcaaaa ttattgaaaa
    18061 agatgaattc ggaatcagtc agtaattttg taaaaagatg ctatcaaccc catcaaatga
    18121 ttgtttttgt ggttgggaac ctgaattttg aacactttgt aaaatggtgt gaagaatatt
    18181 ttgtatcgga aagtgcagaa aatattaacg aaaaacaaaa acgcaatccc cctgaacaat
    18241 atcgggcaga aaaattgaag ctgaagaaaa agacccatca aatccatacc gtaattggaa
    18301 atcgctgttt cgatttgtac catcccaaca ggttgcccat gtatttactc aacaatatac
    18361 ttggggggcc gggaatgaac agtttgctca atttatcgct gcgcgaaaaa cacggacttg
    18421 tttataccgc agaatcgaat tatcaacctt ttaccgacag tggatggtgg tgtgtttact
    18481 atgcatgcga cgaaagaaac gacgaaaaat gtgaacagct ggttttggaa caacttcaaa
    18541 aaatcgttga ccgacccttg ccggaaagta aacttcgaaa atacaaacag caactgatcg
    18601 gacaaatgac tgtttcgaga gaaaacactg aaaatcttgc acttagtttg ggaaaaagtt
    18661 atttgcgctt cgggaaagtg gaagaaatgg aagaaataaa aaaacagttg gagaatattc
    18721 aacctaaaga tttacaatac attgctcaag aaatttttaa tgaagaacaa ctatccgttt
    18781 tgaaatatta ttgaaattat cttcaacaaa aaaatgaatc gaaaatccga aaaggaaaaa
    18841 ccgagtttgc tattttgcct gattatggat atattgggtt gtattaccta cattttgcct
    18901 tgaaaaataa tttccattta ccttttattt cctctgaaaa aagttgcatt aatttcttga
    18961 atttacgtct tttattctct ctttgagaaa cggatattcg ttcaggatat tgtttttttc
    19021 caattcgatt tgttgtagaa aagaaacata ctccggtgaa ttgggatgca gtgggcgatg
    19081 attcattttt tccctcaaca tttccaatcg ttgttttatt tgttttgttt tctccgaaaa
    19141 ataagtatcc gaaaattttt cccaaatgta attgatggcg gtttccgaag gatgtaacat
    19201 gtccgaagca tagaaccgat aatcacgcaa ttcatccatc tgaatttcat aggcagggaa
    19261 ataatcaaca tttttgaatt ttttctgcaa ttcctctatc gataacagca aaatgctttt
    19321 acttaagttg ttttcgtgag cgccatcttt ccaatggcgt atagggctga ctgtaaaaat
    19381 gagatgaagc gatggattca attgttgaag ttgggcgatg agcaaagtgt aatgttcgac
    19441 tatttcttca atggatagcc ttcggcggtt gaaatgttgt gaaggtagct tatggcagtt
    19501 ggaaactaca gtctgtgttt tccggtcttc aaaaacccac gctgtgccaa aggtcagcaa
    19561 aagaaaatct gtatttttca gaaatgaaca agattcatca aaccgggctt gaatattaga
    19621 gagtgttttt tcgggtgaaa catcagaaaa tgaactactg tgaaaaaaac tatgccaaag
    19681 acattggcat tcaaaaatat ctgaacgtga aaaacctttg ttttcaatta atctttcaag
    19741 actcaaagag atggaaagcg gattgtaaag cactccgaaa ggattgacgt caacctgaaa
    19801 gaaagcttca tgaagttgtt tacctatatt ttcagaaaag caagagccta acatcatgat
    19861 tttatcctta taagtaattt tttgttggga tggagcaagt tcaacagggg ttctaaaaag
    19921 atagttagtc ataaaattta ctttattttt aaaaatttgt cagatttcat attagcttta
    19981 acagattata aacgtatttt ccgtaaaaaa tcatttattt attttacaaa agtaataaat
    20041 gttcatagtt attgttgtaa atttgtagct ctaaaaatga tatacaaaat atgaaaaaga
    20101 acaaaaacat cttattgttt atctgttttt tatcgatttc ttttttttgg gggtgtgtgt
    20161 caaaaacagt taaaacaaaa aatttcgagt atgcgaataa attttacctc accgatgatt
    20221 caacaaaagg agctttaaat ttgattatta atgttgagat accagtaaat ttttctaata
    20281 aaaccgtttt ggattcagtt cgcaataaaa tattgttgtt tctatttgga gaaaattatg
    20341 tgaacatgcc tttggattcg gttatcccag cctttgtccg agaacaaagt tccgattatc
    20401 gaaataacaa tctttttatg ttgaatacac tcaatttgca gaattatttt gctttcaaca
    20461 atgaccatat tttggaagga ttttcgttgt ggagtgacga aaatattttt tcatacggaa
    20521 taaaacgtta cgtattcatg ggtggagctc acggattatc aacacagact ttcctgaatt
    20581 ttgatttgaa aacgggaaaa actcttacag aaaaagactt gtttattcct gattataaag
    20641 aaaaactgac tactttgatt cgtgaaagac tgattgagga aaatgacgaa atttcggaat
    20701 atagagattt gaatcaaacc gattattggg tggactccat acagcctaac gggaattttt
    20761 acatttcgga tgaaggaatc aactatttgt ttaatcctta tgaaattggg ccttattatc
    20821 tgggcgaaac agaagttcgg attccttatg ctcgattaaa agatatttta aaaaatggta
    20881 atccgttgga gtatctatat gaaaatcggt aagttaaatg aatatgaatt gttttttcaa
    20941 tttggccaaa tcgcggcgga gcattcgcaa atttcttcct caaaaagttg aacccgaaaa
    21001 aatttcgatg ataacaaaag tggctttgat ggctcctgca tcgaaaagaa gcaatccctg
    21061 ggagtttgtg gtggttcaac aacctgaaat gttacaaaaa ttatcgctgt gtcgtcccaa
    21121 aaattcgcaa tttctggcgg gggctccgtt ggcaattgtt gtgctcgccg atacggaaaa
    21181 aagtaatgtt tggatggttg atgctgctat cgcttccatt atgatgcagt tgcaggcaca
    21241 cgatttggga ttgggcagtt gctggattca tgttcatgac cggcaaatgg atgaaaatac
    21301 aagctcggaa gattatattc gcggtttgct gcaaattcct actcagtatg caattctgaa
    21361 tattctggct gtgggttatc ccaacgaaac aaaaaatccg cacgacgagg aaaaactggc
    21421 ttacgaaaaa gttcatttcg aaaaattcta actttatttt taaaatatgg cttccaaaaa
    21481 gaaaagcagt aatctggcct ggggaataac attactgtta tttggcttgt tgtttctttt
    21541 acgtcaattg cagattgttc cgcccgaaat cggcgttttt gtttttgata ttaaaaattt
    21601 tccgtttatt gccggagtta tttttcttct ttttcatccc aatatcagta tcgggatagt
    21661 tttactcgca gtaggtgtgc tgtttcggct ttcagaaatt attgcatgga caaaaaattt
    21721 ttcggaattt attcttcccg ctttgctgat aattgccggt agcatattga tttttagtaa
    21781 aagaaaataa tcaattgcca caggtgagtc aaattttttg taagtatcta ttccttaatg
    21841 cttttaaatt ctgatacagt tatagttttt tcatcatgaa acgggttgtt attgtcggag
    21901 cgggaaatgt agctactcat ttggggcgag cgttacaaaa taagaatttt gttgtgagtc
    21961 agatttttag tcgtactgaa aattctgcaa cacaacttgc ttctgacttg ggagttcctt
    22021 tcacaacaga cttgcaatta ctcgacagag aagccgacat ttatttttat tgtgtaaaag
    22081 atgccgtatt gtgcgacgtc atcaataaaa tcgatattcc cgacgctttg catgttcata
    22141 cggccgggag cttgccttta agcattttcg aaagccatct gcaataccgt cgatatggag
    22201 ttttttatcc acttcaaact ttttcaaaaa caagaaaagt taattttgat gaaattccta
    22261 tttttgtgga agggaattca ttggaagcag aggaagaaat caggcaaatt gcgcaaacat
    22321 tatccaaaag agttttttct tccgattcgg aacaacgaaa aaaaatccat ctttctgccg
    22381 tatttgcctg caattttgtc aatcatcttt tttccattgc agaagaattg gtgaaaactg
    22441 ccggatattc ctttgatatt ttgttgccgt tgattactga aacagtaaat aaactgaacg
    22501 agctgtctcc ttacgatgcg caaaccgggc cggccgtgcg aaatgatcaa aacattattc
    22561 gtcagcattt gttgttgctt gaaaataaac ccgaattttc ggaaatttac agcgtgttgt
    22621 ccgaaagcat ttatcggatg cacaaaaaat atattcaaac aagagaatag tcaattttat
    22681 tgtaaatcag aaaattgcct tttcttttta tcagctaaat tcaatataca tgaagcaata
    22741 tttgcaatta cttcggttac caaatttact ttttatcgct tttgttcagt ttgccatgtc
    22801 cgaagcggtt attttgccgc ttttgatgac ctatggtttt gatgccggca tgagttcatc
    22861 caaacttttg ttgttgatat tgagtacgat attaattgct gccggaggat atgtattgaa
    22921 cgattatttt gacgtaaaaa tcgataccat caatcgtccc gataaaatga ttgtgggaaa
    22981 aaccattccc cgtgcttctg ccatgcggta ttatcaaatt ttgacggctc tgggattgtt
    23041 gtcagggttt attcttgcct ttcttgtaaa aagtttttct cttggcttta tttttctggt
    23101 ggttccgggt atgttgtggt tttattcggc cacctacaaa aggcagtttc tgacgggaaa
    23161 catcattgta gcttttgcca gtgctctttc cgtacttttg cctgccattc tcgaactggc
    23221 tgtattggaa agtcaatacg gaaaattaat ttttgaaacg cctattccga aacagattta
    23281 tgcttgggta ggaggatttg ccgtttttgc ttttttttgt actttattga gggagattat
    23341 aaaggatgcg gaagatattg ccggcgaccg ggaaatggaa tgtcatacct tgccaattgt
    23401 ttggggaata aaaaagacca aaattattgt ttattgcctt attttgctca ttgtttccgg
    23461 cttgctcgca gtcgactttt tcctcattga ttttagcagc agccttacta ccagatacct
    23521 gatttttggt ttgataattc cttttgtttg cttggggtat ctgattttta aagctgaaaa
    23581 tgcccgcgac ttccatcaag cttctactct ttcaaaattt atcatgttgt tgggtgtttc
    23641 ctatagtttt gtgttttatt ttttgcaggc gcagcagtac catttcccta tttttcatat
    23701 ttttatggtt caataaatcg ggaaaaatca tccatcactg tcattcattg acaattggta
    23761 atcgggcttt atcgttttat gataaaattg aactaacacg tttttggctt tttctcaaaa
    23821 aaaaaattca cctcaaaaga atttacagct atgttaatgt tactttctcc cgcaaagatt
    23881 caaaacacca aactcccaaa aaattcatat cctgccacca taccgcaatt tttggatgac
    23941 gccgaatatc tggttgaaaa agttcgcgaa ctttcactgc gggaactggc tcaattgctt
    24001 ggggtcaact cacaattggc cgaactcaat cgcgatcgtt tttacagttg gcatcgccct
    24061 tttactgtcg acaatgcaag gcaggctgtg ttggtttaca acggggaagt ttttagagga
    24121 ttgaatgctg cttccatgac agaaaatgag ttaacctatt tgcagcagca tttaagaatt
    24181 ttatccggtt tgtacggcgt tttgcgtcct ctcgatctta ttcagcctta tcgcttggaa
    24241 attagcagcc gcttgccaac ttttgcaggc aatgatttat acgatttttg gggaaagaaa
    24301 atcacagatg ccgtttccca agcttttcaa ttttccggtc aacctgagat tttacttaat
    24361 ctctgttcca gtgagtatat taaagccata gacagagatt ttttcgatgg taaaattgtt
    24421 gatatcgaat ttttggaaaa tgatcatggg aattttcgtc caattgtcgt ctatttgaag
    24481 aaagcacgcg gcttgatggt gcgctatgtt gcccaatatc ggatagaaaa tgtggaggat
    24541 ttgaaaggtt ttgatgttga agggtattgg tatcatccgg aattgtctaa cgacaacaaa
    24601 atggtttttg tcagaggata aggcaaattc tctcgttgtt tttccacaaa tctatgccga
    24661 ttgaatctca tttaaagcaa aatcaaacaa tttagaaata aattaaaaag acatttagat
    24721 acatttttgc ttcaaaagtg gaagcatttt ttggctttat caaacataaa tactactgtc
    24781 cgataaaaaa atagaaaagg aaagaataaa aaaggggaag ttactcaaat tagataactt
    24841 ccccgacata tagtattttt gtttttgcac aaacataaaa actatatggg caaaaatcca
    24901 gaaagaaatt tggtctttca gccggacttt cggattgaca tttaatgaag ttcttttgat
    24961 tgatcttacg acgataaggc tatttaggga cattctcaag gaagttgggc gaaatgccca
    25021 aaaagcccga cacaaaaaaa atggtttttt ccattgtggc aacattagta cggatagatt
    25081 taatcaattt atgaattgta cgccaacagg ttttccatca aatcgacaaa ttcatattca
    25141 ggcttgggca caaacaaact ggcagaaatt tcacccaatt ttctttttgc tgcatctatt
    25201 gccagagatg actggtcaat gcctatccaa tttctgttgt ttaagtgagc tgattttaaa
    25261 gttgttcctg acccacaaaa acaatcgagt acaatactgt tttcgttcga ggaagtttta
    25321 ataattaaat ccagcatatc tgaatttttt tctgtaggat aatcgggata ctgaggatcc
    25381 ttaaattccc aaatatcctg cacccttttc ccttcacttt catctgcata aataattttt
    25441 ctcggattac ctgtcgaaga ccattcgatt aagccttcat tatcccattt ctcaagcgtt
    25501 ttaatgtctg tacgccaatg ccttccgggc ggaggcaata aacctttaaa aggtttactc
    25561 gaatctccac ttgatgtctc accaggagca tgaatcggaa cggttgtgta tcgccttccg
    25621 tttttgtctt ttttcggata aagtttttcg atattaactt ctttatgttt ttcttttggc
    25681 tcattccata taggatttga tgttttggta tagaataaaa tcatatcctt aatattccca
    25741 taacctatgc gagaaaaatt tttcggatta catttaattc ttgtaatatc gtttctgaag
    25801 ttttctacac caaaaatttc gtccatcatt actttaacgt aatgtcctat tttataatca
    25861 gtatgtaaat aaattgagcc tcgttctgaa agcaaatctt ttaaaatttc aagcctttgc
    25921 cttaaaaact caataaaatc actacctttc acgttatcgg aatatgcaac atctccattc
    25981 ttagaattgc tgatagttgt tgccctcccg ttagaaataa caaaattgcc tcctgtagca
    26041 aatgggggat caatatacac caaatctatt tggcccttta attccttttc ttccaataaa
    26101 tacttaagtc ccttaagatt gtctccgaaa ataagtaggt tgctcataga tttataattg
    26161 gtaaagaaat tctcttaata ctaaagcgct cataatgttg taattattaa acttttgagt
    26221 gatagattta tacattttgt tgtttccctt tatatataaa acaccatcaa gaatagccac
    26281 cttgatcgct ttaacttttt taacttcaag agtagaaatg gcatcgttaa attgcgcatt
    26341 ttgatgtcct ccgaaatcag ttaaaaactt cgcttcacct attacatact tcccattgaa
    26401 tcgggcaaca aaatcaagtc ctttatcatg ataataaccc agcttttctt tagcaaaatc
    26461 cattaaaact ttatcgcccc catccaaaat cgcatcatta ttaccggaca taaattcatc
    26521 tatttcaaca ggcaaaattc ctaaagattt gtttcgcaac caatctttga acattggccc
    26581 aatttgtcga ttcgtttctt tgggctcgct acatctttca taaagtttat tgagccctaa
    26641 ttcataaatt cttccacaaa ttctgttgat ggttcttggg ttcctatcaa tagctgaact
    26701 atctcttttc aaatacgcaa tataactatc ttttatagga aaaagatcca attttaaaag
    26761 ttcttttatc agtcgataat tgtccctctt tataaaggca ttttcaacct ctctccatat
    26821 ctgcacatct atttctcgta ttccttccgg aattgttgga tatacttgaa ataaatcatc
    26881 gagatatgat ttttgattgg catattcaat actaagatta atccatttat tcatttataa
    26941 ataattttta aatatcaatt tgaaaaactt gctcatatat ttttcccctt ccgaatacct
    27001 tacaaattta agcgaaaatt ctcaattttc ataccgtcaa aagctcgttg attttttaag
    27061 catattcatt caggcaatta gtattattcg ttagattaca aaattttatt ttaaaccgac
    27121 tggcaaaaat cttataatta aattttcccc gaacaaaaga attcttgaaa aaaattcctt
    27181 ttgtttctgt aaatttacaa caattttgag gcttttgttg ttaaagtcaa atgattaatt
    27241 cgatttattt atagaaaatg aatacgttag tggcagcacc aaaaaaaatg ggaataactc
    27301 aattagttga tattgactta aaagaaattg ttccttttat cgactggaac tttttctttt
    27361 tagcatggag attacctggc aaatatgaag gaatcgaaaa tctttgcaat tgtacctcct
    27421 gtgaagtagg gtggttgcag caatttccgg aaaataatcg taccaaagct gaagaagcac
    27481 tcaaattgtt ccgcgatgct cagcaaatgc tccaggattt tcttcatgag aagtcgatat
    27541 ccattcatgc catttttggt ttctttcgtg ctgtttcaca aggcgatgac ttattaataa
    27601 gttttggcga ccaaacgtat attttgccta cacttcgcca gcaacacgct tcatcagacg
    27661 gtttttgcta ttcgttggcc gattttataa atacagaaaa tgattatatg ggagcttttg
    27721 caacgactgt agaaggtgcg gaaacgatgg ccgaaaattt tgagagagaa gacgatttat
    27781 atcgtgccat tatggttaaa acgctctccg atcgactggc agaagctgcc gccgaatggt
    27841 tgcattataa ggttagaacc gaattttggg gctatgcacc cgacgaacca ttggagataa
    27901 aaaacatatt gaaaggaaaa tatcagggta tcagaccagc agttggttat ccttctcttc
    27961 ccgatcagtc tataattttt gagttacaat ctttgttggg actcgaaaaa attggaattc
    28021 gcctgacaga aaatggagcc atgtttccca atgcttcagt gtgtggtttg tatttttctc
    28081 atccaaagtc gagatatttt atgattggga aaataaatga tgaacaactg gaagattatg
    28141 cccgaaggcg gggaaaaacg gttcaggaaa tgaaaaaatg gctggctgca aatatttaga
    28201 tttaattgat taacaacaaa aaatagctaa atataatttc attattaaaa atttcttgaa
    28261 aaggagataa aatcatgtta gaaaaatcat tgaaaatcaa aggaatgagt tgctctcatt
    28321 gtgcaactcg cgtagagaag gcacttaatt ccttagatgg cgttgaagct aaagttgatt
    28381 tggcttccaa ctcggcaaaa cttacacttg ccaaagaatt gagcaacgaa gaaataaaaa
    28441 aggctgtgga aaatgttggt tatgaagtga cagaaatttc ggaataattt cggcttgtca
    28501 aaaagtttta aaaatagggt tgcccgaaaa acggacaacc ctatgctgcg agaatgtaaa
    28561 acaaaacaga taaaaaatca aaactttaga aaatcaacag acaaataaat tttgaatagt
    28621 tttgattgtg aaaatttcat tctgcaaaga tttattcaat caagattgac gtcaaaaaca
    28681 tgttaatttt ataagaagct tgaagaatta ttcaaagata gttcattcga aaatttttgg
    28741 ctacgcagat ggtaaaattt tatcagtaaa aggtaaaatt ttgtagcaaa tttgtttttt
    28801 tatttcattt ctttagttta attttgtttc agatgaaata aaattatgct aaaaattatt
    28861 ttgttgctct ctccggttta tgttactctt ttttggtgta tagttttaaa cactcaagga
    28921 gataattatt ctgctcccaa gtcttttttg gggaagttca tgtttttttg ttttattgta
    28981 tattcatctc atttcttgta ctttatgaaa ttagaggaag cttatagctg gatcgatcct
    29041 ctatatcaat ttgcttcttt gctggtttat cccatgtatt acatatattt tcgtttactc
    29101 accgtcgaca gaaaattttc atttaaaaaa cattttgttt atctgctgcc atccactatt
    29161 ttgtttgtgc tttattcgat agctgtgttt tttactcctc aagaagaata caaagcattt
    29221 cttttcggcg aagaacatca ctcactgaat catttaagct atattgaaat cgttagtttc
    29281 ctgatacgga tagttttttt gattcaggta atattaacgg taataggcaa ttttcgtttg
    29341 attaggaaat atggacacaa ggctgcacaa tattattcca atatggaaga cagcagcatt
    29401 tccaaagtca gattactgaa ttatacgctt atcatcaccg ctatttcttc ctttattctg
    29461 ggaatgctgg gtcgtggatt tttcgcctcc gaaataactt atctggctat agcttcagta
    29521 gttttttcgt ccatgctgtt tgcaatcgga tggcttggga ataaacaaaa atctttaaat
    29581 cctgcttatg aaacagacag catcactgaa accaaacaaa aatccgaaga actgtccctg
    29641 atccgccagg aagaattact aaataaaatt gaatatttct tcaacgacag aaaaatatat
    29701 ttgaacagca gccttactat tttggatgta gcccgattaa ccggcacgaa tcgaacgtac
    29761 gtttcctcac tcattaatca tcattttcac caaaatttca gtgcatttgt aaatagttat
    29821 cgcttaaagg aactggaaga aaaaatttta tttaatccgg agtatcccca tcagattttg
    29881 gcagaaactt gtggtttcgg atcgattgat tctatgaaac gtgccattaa tcttagatac
    29941 aagctgtcgt tgcaggaatt caaatcagag gttctcctca aaaaagtggt ttaacgggtt
    30001 aaacaaacaa taaaaatact ttactattct caagcatcaa agtttttaaa aattctcaac
    30061 aatcaatcga aataatttac tgagacctaa atttttcaca atttcatcag aatataattt
    30121 attttttaaa ggtctgatgg aacccacatg tctgaatatt atattcattc gaatttgtgt
    30181 ttgcaataca tgtgggtttc atttgattaa aaacttgtta tattttcctt ttttatgaaa
    30241 aatttttgag aaagacctgg aaaaaataat taaaaacatt tatctttgca gtgtgatttt
    30301 tttcatggta ttagatttaa ggttaataag gaagattggt ggtcaggcgt ggctaccatt
    30361 cttttttata gcaatttttt tcgtattaat tgctttttca ataaggatgc cgtacatgtt
    30421 aacaaactgt tcgcctgctt taataacagc tctttttgaa atttcttgtg catgattact
    30481 gggctttcta attttattgt ttccattttc tgttattttt agtggttatt ttgatatcat
    30541 aaggataata gatatatttg ttatatagaa atttttagtg ttaaattttc gacctgaaac
    30601 tttgttttat gattaattaa cgttttgtct ttcatgtatt tgcataaaaa atgatggaat
    30661 tttgttgcaa taaaaaaagg cggtgttacc cgcctaaaca tccgattagc cactcgcatc
    30721 ccaccgcctt gtttcatgcg caaatagcct tgtccgctaa tcagaatttc ttatattgct
    30781 ccttagttaa tatcgtacct cccatgtcgg ctctggttac aatatttgaa gtttcatcaa
    30841 tcttatattc ccttcctcct ttaaattttg caaacgtctt gtaaccgtct tttgaatagg
    30901 aatatcgtat tgcgacttct tcaatatcgt ccatgatgta aatctcattc cctttttgga
    30961 agttttcgta aatgtaatct gtgttgttca tctaaatttg ttataatatt ttgttgacaa
    31021 atctattagc tcatttcttc tttttctgcc ttctttagat attttagatg tacttctgta
    31081 ttcctcataa aatttatgcc ccaatccacc ttcaagacct gtttctttgt gtatttcatt
    31141 ccaccttttc tcaccaataa ttttttttgc atcttctggt ttttctttcg caaatatcat
    31201 tcggtcggta ttaaattgaa tctctccgac gattccatct tttgttctta catttgctaa
    31261 cactcctgaa tagccttgaa atttttctgg tgtttgattt tttacacgta taaagatagt
    31321 atttttttat attatcaata atcaaccaag aaaaagattt aattacgaat cgcctatttt
    31381 tatatttaat caaaaagttg cattttctac ttgaatgttg ccaataaaat attctgtatt
    31441 tttctattta tctatttttt cagaaagtta cattacgcaa tttttaatcg gtgaacattt
    31501 gttttttcga ttttactttg agcgtaaggg agcgtaacca cccatttggt agattttttg
    31561 gatggcattt cgtacatcga atccatcatg atggtttcgg taatggaacg cagacctctg
    31621 gcgcccaatt tgaactctat agctttatcc acaatgtagt ccaacacttc atcttcaaaa
    31681 atcaattcta tgttatccat tttgaatagt ttgacatatt gtttgataat cgaatttttt
    31741 ggttcggtaa gaatgcgacg caacgcatcg cgatcgagcg gctccagata agttagaatg
    31801 ggcaaacgtc cgataatttc cggaataagg ccgaaagatt tcaagtcttg cggagcaata
    31861 tattgaagaa gattgtttct gtcgatgagt ttgttttctt ccgaagctcc gtatccgacc
    31921 actcttgtat tcaatctggc agcaattttc ttttctattc catcaaaagc gccaccacaa
    31981 ataaacaaaa tgttttgcgt gttgacggga atcattttct gttccggatg ttttcgccca
    32041 ccttgaggcg gaacattcac caccgatcct tccaacaatt tcagcaaacc ttgctgaacg
    32101 ccttctccgc ttacgtcgcg agtaatactc ggattatcac ttttgcgggc gattttgtcg
    32161 atttcgtcga taaaaacaat tcctctttcg gccgctttta catcataatc ggcaacctgc
    32221 aacaatcggg tcaaaatact ttctacatct tcgcccacat aacctgcttc ggtgaaaacc
    32281 gttgcatcaa cgatggtgaa aggtacatgc aactttttgg caatggttcg tgccagcaac
    32341 gttttacccg ttcctgtggg gccaaccatg ataatgtttg atttttctat ttcaacttca
    32401 tcttcttccg ttttctgcat caaacgttta taatgattgt agacagccac cgaaagatat
    32461 ttttttgctt cttcctgacc gataacatat tggtcgagat actgcttaat ctcttttggt
    32521 ttgggtattt tttcgatatg cagatgggaa gatttggact ttcgggcact ttccctgaca
    32581 atctcagctg cttgaatggc gcattcatta caaatttgca cgccatcctg acctaaaatc
    32641 ataaattcta cttcagcttc agagcgtccg caaaaattgc aatgtctaac gttttttggc
    32701 atacttgtaa tattattttt tactctttcg ctcgttaatc aacactttat ctatcattcc
    32761 gtattcaagt gcttcctgtg aagtcatcca ataatcgcgg tcggcatctt tttcaatttt
    32821 ttcgtaagga tttcctgaat gttcggcaat aatgttgtaa agttcctgtt tcattttttg
    32881 aatttcgcgt gctgtaattt ctatatcgga agcttgtccc tgagctccgc ccataggttg
    32941 atgaatcatg atacgcgagt gtttcagggc aaaccgtttg cctttggttc ctgctaccag
    33001 cagaatggcg cccatagacg ctgccatacc cgtgcaaatg gttgaaacat cggaactgat
    33061 atactgcatg gtgtcgtaaa ttcccaatcc ggcataaacg gatccgcccg gagtattcag
    33121 gtaaagagaa atatctttcc ccggttcggc cgaatcgagg taaagcaact gtgcttcaat
    33181 aacgtttgaa gcgtaatcat cgatttcggt gcccagaaaa ataattctgt ccatcattaa
    33241 tcgggagaaa acgtccattt gtgtaacatt cagctgacgt tcttccagaa tagtgggaga
    33301 aatatagctg tcattaataa aaccggcata ctgatcgata gctaaaccgt tcattcccag
    33361 atgacgggtt gcataattgc gaaaatcgct tcttatattt tttttgttca tattgtttcc
    33421 tttcttttta atttaaggtt tttttttaat atctacctga aaaagttctg atataaacaa
    33481 attaaaaagg tttttgtttt caatgaagtg ttttcaatga aaattttttt aattttcctc
    33541 aaataatttg tcaaattctt caggactgat ttcttttacc tctacttcaa cactatcttt
    33601 tatggttaca ataactttat tgtcgcctgc gcgctcgatg aaatttctca gggtatcttc
    33661 cttttcaagc aagctcttgg catagttgga aattaattct tcgtcggctc cgcttatacc
    33721 gtactgagca agctgtgctc tggcaaattc tttggcgtaa ttttcaactt ctacttcatt
    33781 cactttgatg tcgaattttt ttatcagctt ttccttgata agttgccaag tgaggtcttc
    33841 aatcatttta gggtaatccg attccaattg ttcgttggtc aaatttttgt tggaagccaa
    33901 caaccagcgt ttcaggaaag catcagggaa agaaagattc ttgaattttt ccaccaatgt
    33961 tttcttggca tcaatgccaa atcgatagtc gctgttttct tttaaattgg tggcgatagt
    34021 ttccttgact ttttcgcgaa attcctgttc gttggttact gcattttctc cgaaaacttt
    34081 atcgaacaat tcctgattga tttcgctatc gaagtagcgg gtaatatcgt taattttgaa
    34141 actgaaatcg gaagtaattt ctttagcctg ttcctttgaa attttcagga atgaagccag
    34201 ttctgtttca ttgccgaatg ctttggctgg gttgaagata acggtatcgc ctttttttgc
    34261 atttaaaaac aacgcttttt gttcaggatc ggcgatgtag gctggtgtga ggatagcttc
    34321 cgaaacttgc aagccgtttt gcttttcttc accgttttcc aattcaatga cttcgccccg
    34381 aagcatatcg ttttcttcta ccgtttccac ctcttcgtag cgaccaaatc tttgactata
    34441 gctttttatg cggtcttcta tcatttgatc ggttacagca atgttgtaat aatttatttt
    34501 gatctctttc gacaattcta tatcaaaatc gggagccagc cccagatcga aaacaaaatc
    34561 aaactcttcc tgagtattga aatctatttc gggctgctca gtttcgttgg gcagtggatc
    34621 gcccaatata ttcaaatcgt tttcgcgaat ataatcataa agtttttctg caatcagctt
    34681 gtttatttcg tcagccatga cagatttgcc atacattttt ttcaaaaggc tcatcggaac
    34741 cattccgggt cgaaagcctg gaatattggc ttttttgcgt aaatcgcgaa gatttttttc
    34801 aacattttcg gcgtaatcgg ctttttcaac gtgcagggtt actactgcat tgttttgatc
    34861 gaggtctttt ttaacaatgt tcatactgtt agttttctga tatttataat tttt
//
LOCUS       DAOSIB010000014        32650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_1545_length_32650_cov_11.568891, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000014 DAOSIB010000000
VERSION     DAOSIB010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 32650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32650
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1545_length_32650_cov_11.568891"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1308)
                     /gene="argG"
                     /locus_tag="PLD44_02405"
     CDS             complement(109..1308)
                     /gene="argG"
                     /locus_tag="PLD44_02405"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOF98222.1"
                     /translation="MKEKVVLAFSGGLDTSFCVKYLSEEKGFDVYTAVANTGGFSDAD
                     LQNIEKRAYQLGAVKHVVLDVTKDYYEKCIRYMVFGNVLRNGTYPISVSSERTFQAIA
                     IINYAKEIGANYVAHGSTGAGNDQVRFDLTFSVLAPEIKILTPIRDLALSREYEINYL
                     RQHGFEADFKKMEYSINKGLWGTSIGGKETLKSDQTLPEEAYPSKVEKQGEEILTIEF
                     EEGEICGVNGQKNSDKIALINLVEKIGSQYGIGRDMHVGDTIIGIKGRVAFEAAAPML
                     IINAHKLLEKHVLTKWQQYWKEQLGNWYGMFLHEAQYLEPVMRNMEKFLESSQQNVTG
                     TVILKLRPYCFTLVGVDSPFDMMKTDMGEYGEVNKGWTADDVKGFTKILSNQLKIYYT
                     LQNKNQQ"
     gene            complement(1403..1999)
                     /locus_tag="PLD44_02410"
     CDS             complement(1403..1999)
                     /locus_tag="PLD44_02410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF98223.1"
                     /translation="MENNFSMDEITVQVADESYIKYVDTILKTIEEAAKIRGTGIARR
                     SPEYIEQKMKEGKAIIALHGEEFAGFCYIETWSDKKFVANSGLIVVEKFRGHGLAKRI
                     KQKAFELSRERYPDAKIFGLTTGLAVMKINSELGYKPVTFSELTNDEAFWAGCQSCVN
                     YDILQRTGRKYCLCTGMLFDPAAEQKKSSTPTSEKEEK"
     gene            complement(2070..2552)
                     /locus_tag="PLD44_02415"
     CDS             complement(2070..2552)
                     /locus_tag="PLD44_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HOF98224.1"
                     /translation="MKNKRNRLKIISDILRSNVVGSQEELLKLLKEQQCEITQATLSR
                     DLKVLKVAKTPLSNGTYKYVLPSYNKPLPPPSANTLNGSLANGVILSLEFSQQLAVLK
                     TKPGYASAIAWDIDNKASDYILGTIAGDDTILVVPREGVEREVVVEIMNDIFGEPKAV
                     "
     gene            3026..4225
                     /gene="coaBC"
                     /locus_tag="PLD44_02420"
     CDS             3026..4225
                     /gene="coaBC"
                     /locus_tag="PLD44_02420"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOF98225.1"
                     /translation="MLQDKKIILGITGGIAAYKSATIVRLLLKQGAEVKVVMSASAKE
                     FITPLTLAALSKNPVLTDFFDPTNGQWNSHVDLGLWADAYLIAPATANSLAKMAAGIA
                     DNLLLTTYLSAKCPVFLAPAMDLDMFAHPATQRNLQILRSYGNLVIEPSFGELASGLE
                     GKGRMEEPENIVHFLDDYFTPKQLAGKKILITAGPTYEKIDPVRFIGNFSSGKMGFAL
                     AESCAAHGADVCLIAGPVQLSLQHKNIQLIKVSSAQEMFDAVTEKFPEMDGTILSAAV
                     ADFTPVAIAEDKIKRKGDRLTIELKPTPDIAATLGKIKTEKQFLVGFALETSNEIENA
                     QLKLKNKNLDFIVLNSLRDEKAGFGYDTNKITILHNTGKSVSFDLKPKKEVAEDIVNE
                     LVKILNR"
     gene            4222..5124
                     /locus_tag="PLD44_02425"
     CDS             4222..5124
                     /locus_tag="PLD44_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOF98226.1"
                     /translation="MKKKFAILIFLSFCFSFHAYSQELNCNISINSDQVQGTNKSVFN
                     TLQKSVSEFVNNRKWTEMTFLTNERIECSMNIIVKSVDGDNFTAEIQVQSRRPVYNSN
                     YNTTLFNFKDNDFTFSYKEFEPLEWNEASISSNLTAVLAYYAYIIIGYDMDSFSRLGG
                     TPFFQAAENIVNSAQGSNLPGWRAFESSRNRYALANNLTDEAFKNFRNYFYEYHRLGL
                     DEMANNPVNARARIAQGISVLREANRARPSAVIISTFLDAKTDELINIFSKGTDKEKA
                     LVVEILSDVNPTQTQKYEQILNKK"
     gene            5151..6809
                     /gene="recN"
                     /locus_tag="PLD44_02430"
     CDS             5151..6809
                     /gene="recN"
                     /locus_tag="PLD44_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOF98227.1"
                     /translation="MLKTLHISNYALISEINIGFNSGFSVITGETGAGKSIIIGALSL
                     ILGQRAESRFIKEGEEKAIVEAEFDIRDYRLEHFFEINELDFHPEQCIIRREITASGK
                     SRAFINDTPVSLQLLRELSAKLIDIHSQHENLLLSDDQYQLQVVDNMAQNASILKQYQ
                     QYYSQWKNLQTQLSQLQKTADKQLSELDYLKFQFQQLEEAHLSENEQDDLEMELETLS
                     HTEEIKQELQKAVFLLTDEQKSLVLLKEVIDAVGRIKSYISEGENWHKRLESVYIELK
                     DIAQEITSLEENIDFHPDRLEWVDQRLNEIYTLEKKFKLSSISELLSLKENLKEELQH
                     IESFDEEIDALKKKIAEAHKNVHQQAELLTKSRLNAVEPIEKFLKEQLSRLGMPNIQF
                     KVEITDAAEFSETGKDTVQFLFSANKNQSLQPVAQIASGGEVSRLMLSIKSLMAHRAG
                     LPTIIFDEIDTGVSGEIAHRMAEIMKEMSKNMQVIAITHLPQIAAKGNQHYRVFKDDS
                     GKTTQTHINLLDREERIWEVAQMLSGENVTEVALGNAKELLKSE"
     gene            6873..7748
                     /locus_tag="PLD44_02435"
     CDS             6873..7748
                     /locus_tag="PLD44_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOF98228.1"
                     /translation="MIQSMTGFGKATCEYEGKKIVVEIKSLNSKQLDLSLKIAAAYRN
                     KELEIRTEILQKLERGKIDVAIYIDNSGKDVANQINPSVFLSYMQQIKSLSQNLELPE
                     PTNWFDILLRLPDVMKTDNIELEENEWQALKKSIDKAIEQVISFRIQEGKSLENVFIS
                     KITNIGDLLEKISPYEAERIEKIRSRLKDNLQALSDKVDYDQNRLEQELIYYIEKLDI
                     NEEKVRLRNHLNYFFETMKLEPSAGKKLGFIAQEIGREINTLGAKANHSEIQKIVVKM
                     KDELEQMKEQVLNVL"
     gene            7813..8379
                     /gene="gmk"
                     /locus_tag="PLD44_02440"
     CDS             7813..8379
                     /gene="gmk"
                     /locus_tag="PLD44_02440"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOF98229.1"
                     /translation="MSGKLIILSAPSGTGKSTLVQYLLTRPFDLEFSVSATSRPPRGS
                     EQNGKDYYFLSKEEFQKKIAQNEFLEYEEVYPGCYYGTLRSEIDRIAAKGKNIIFDVD
                     VLGGLNIKKQYNGRALAIFIAPPSLEELKNRLTIRGTDPPEMIAKRLEKAEFEMSFAP
                     KFDVVIVNDDLEKAKKATEEVVRNFLEK"
     gene            8393..8971
                     /gene="nadD"
                     /locus_tag="PLD44_02445"
     CDS             8393..8971
                     /gene="nadD"
                     /locus_tag="PLD44_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOF98230.1"
                     /translation="MKKVGLYFGSFNPFHLGHQKLAEFLLDHQVVDEVWFVVSPRNPL
                     KKENDLLDENLRLEMVKIAIQDNPKLKVSDLEFSMPKPAYTIDSLHAFSSKYPENDFY
                     LLIGSDNAKVFDQWKNFKQILDEYPVLVYPRPGFDKEEIAKIYPQMKWLEGPQYNISS
                     TEIRQNIKQGKEVKDLLQPAVYQYIFKKKLYV"
     gene            complement(8974..10860)
                     /gene="ftsH"
                     /locus_tag="PLD44_02450"
     CDS             complement(8974..10860)
                     /gene="ftsH"
                     /locus_tag="PLD44_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOF98231.1"
                     /translation="MTKRAKYSWIYWLVIMFLFILMDWLIFPTTQGNRELSYNEFVDS
                     VRNGKVERIIFLQDKIVGEFKSDTNHISSMTQAPTAPWRIRIPDLEEQVQKQFVVSRI
                     PNMNEEYLINDLLAAKVNFKGHFQDNTVLNFFLNWILPFVLFMLLWGYFFRKMGNKNP
                     MLEIGRNKAQIQAEKPANPITFKDVAGIDEAVEEVKELVDFLKNPSKYTRLGGKLPKG
                     VLLVGPPGTGKTLLAKAVAGEADVPFFSLSGSDFVEMFVGVGAARVRDLFNQAKAHAP
                     CIIFIDEIDAIGKSRAHSGYYAGGYDERENTLNQLLVEMDGFDASSGVIIMGATNRPD
                     VLDPALLRPGRFDRQVLVDKPDMNGREAIFKVHTKNIKLASDVDLKRLAAQTPGFAGA
                     EIANVCNEAAILAVRNNRDEVTMMDFEAAIERVIAGLEKKNKLINEKERKIVAYHESG
                     HAIVGYFTPGADEVQKVSIVPRGIGALGYTMQMPLEDRYLLSESELIGKIKGLLGGRA
                     AEELIFGEVSTGASNDLERATQLARNMITVYGMSKKLPNISLVNQSTTGFLGGGPGIE
                     RRSEYVEKMIDEEMTEIIRSCYENAKKLLSEKKDLLEKMAITLLEKEVLSYNEIKEIL
                     GEKN"
     gene            complement(11041..13266)
                     /locus_tag="PLD44_02455"
     CDS             complement(11041..13266)
                     /locus_tag="PLD44_02455"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOF98232.1"
                     /translation="MENKVFFLVPVSAVLALFFAWYFFHQMKKESEGTISMQKIAKYV
                     REGAMAYLKQQYKVVGIVFLIITIIFSFMAYYLKIQNPWVPFAFLTGGFFSGLAGFIG
                     MRTATYASARTANAAQQSLNAGLRLAFRSGAVMGLVVVGLGLLDISIWFYVLQFVIGA
                     LDNATNLTIASDSSMKLIIITTTTLTFGMGASTQALFARVGGGIFTKAADVGADLVGK
                     VEENIPEDDPRNPATIADNVGDNVGDVAGMGADLYESYCGSILSTAALGAVAFAAKGT
                     AVQFNGVIAPMIIAAVGIILSIIGIFMVKSKEDASQKQLLSSLSRGINFSAIFIAIFS
                     FLILKYLNIPNYLGIWGSMLAGLFGGVAIGKITEYFTSAGFKPTQRISESSKTGPATV
                     IIAGIGTGMISTAFPVLVVGISIILSYLFASGFDFSNISMGLYGIAISAVGMLSTLGI
                     TLASDAYGPIADNAGGNAEMSGLAPEVRRRTDALDALGNTTAATGKGFAIGSAALTAL
                     ALMASYLEEIKIAIARIPEKTELFFNAMQTDIHQASIPQLIEYFQINLMNPRVLVGAF
                     IGAMAAFLFCGLTMNAVGRAAQNMVQEVRRQFREIAGIMEGKAEPDYARCVAISTKGA
                     QKEMLLPSLLAIIIPILTGVIFGVSGVMGLLLGSVSTGFILAIFMANSGGAWDNAKKY
                     IEEGNLGGKGSENHKAAVVGDTVGDPFKDTSGPSLNILIKLMSMVSIVMAGLTVAWSL
                     L"
     gene            complement(13340..14581)
                     /locus_tag="PLD44_02460"
     CDS             complement(13340..14581)
                     /locus_tag="PLD44_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOF98233.1"
                     /translation="MSLVEILFYLILFFIVGDFILERYLSFLNFQYSKNELPDNLKDI
                     YDADKYGKQQEYFRSNIRFGTISSTINFILILIFFCLGGFGWLDTLLRRITEHPILLP
                     LLYFGVLFVAETLISIPFQWYDTFVIEQKFGFNKVTPKLFVQDHLKEFALSVFLGGIL
                     LGAIVWIYQLTPKYFWLIAWSVVTLFSLFMSMFYSQIIVPIFNKQKPLEEGELRDEIE
                     KFATKAGFKLDNIYIIDSSKRSTKANAYFTGLGNKKRVVLYDTLLDKLTSEEIVAVLA
                     HEIGHYKKKHTLYQFLISLPESLLLFFLLGLILKSDVFAQVLGGTTASFHLNVIAFGL
                     LYTPVSLLLSICTNILSRKFEYQADAFAANFGWAKQLQSGLKKLAATSLSNLMPHPLY
                     AFFHYSHPTLYQRLTKLSGAQ"
     gene            14742..15701
                     /locus_tag="PLD44_02465"
     CDS             14742..15701
                     /locus_tag="PLD44_02465"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF98234.1"
                     /translation="MKRLQIFLFILITLFSLSCSNKTASDKISIAIPEGPSVVSFIKM
                     MDDSVSIEGKKVEYIVKSDPQQIQALMMQNKVDFAILPTNMAANLFNKGIKYKMIACP
                     VWGTLYILSNLPEVKTLNDLQNRKVSIFGQGTTADILFQKLIQKKGIEKVFIDYRFTG
                     NFELSQALRLKKTNIAVISEPLVSKLLEQDSTIHIVEKLSCEEYLNEKDRDIFVQTAF
                     LVNEKLERNNPQIIKEICNAYRASCNYVTQHPEEAAVLLVKHGILPETNVAKKSILLC
                     SINYVAAFAIEDEIYSYLKIFYDFNPESIGGKLPSENFIFQVF"
     gene            15713..17113
                     /locus_tag="PLD44_02470"
     CDS             15713..17113
                     /locus_tag="PLD44_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF98235.1"
                     /translation="MKNQNKKIFPFILSVFSLAIIWQIISISIGYPAIFPSIFDLISD
                     TLHLFTRSEFYFALLGTVSRGLLGFVITFFIALFFGSLSVFNSFFKDFFHPIVVFTRS
                     IPVISIVLVALLWFSPPFLPVFVAFFSMFPILFQSIQSGLENVDKKWVEMGKVFGLSS
                     RKRFLHIYYPAARSTIFDGISSAMGFGWRAVIIGEALAQPVHGIGSQMKAAQAYINVS
                     EIIAWTLAAILISYIFEKIILFLKKCNRPIQLPKSEECKLHKFNQIVDKKEITIYNLK
                     KKFNDYTVFSSFNENFKDTEINFIKGPSGIGKTTLLRLIARLEKPDEGTIYYSSKFSF
                     GYSFQDVRLLPWLTVKENIAFPLLYKKTDRQELTDNIDWLLNNMELTEHQNKFPDQLS
                     GGQLQRVGLARALVAQPDVLLLDEPFTGFDDDLKTRIQDFLSKWFEDFQPLVIWATHE
                     NILFPEKNRREIQLKN"
     gene            complement(17221..17742)
                     /gene="rimM"
                     /locus_tag="PLD44_02475"
     CDS             complement(17221..17742)
                     /gene="rimM"
                     /locus_tag="PLD44_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOF98236.1"
                     /translation="MISFTEVIPIGQIIKLHGINGEMAFMSNYEGLDFDNIPFLIFDM
                     HGILVPFYLKEWRFNSENSGFLKIEDVNNEKKAKNFLGLTIYLHKKFLNILESDVDMD
                     NNTFIEFRIFDKDNNFIGIVTDIDTSTQNVLFIVDNGEKQILIPACQDYILQIDEMKK
                     IIYMDLPEGLLEL"
     gene            complement(17811..19568)
                     /locus_tag="PLD44_02480"
     CDS             complement(17811..19568)
                     /locus_tag="PLD44_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98237.1"
                     /translation="MKKKLVFLSIFSCVNFAISLAQDSVFTRNITVEREYQPILKNTG
                     KLNVTPKLIEPEAKPLPAQYTDLFIPLSINNNIHPLSSTEFFFNNRVEQNNFLQLGLG
                     NFYNSSANFLYSLLNRPNTKLDFYMNHIGTFGDKLHSTTKSGLVFDQQLKSLHLFAGV
                     ETQHERLKFYGFNFNDSASVQLNELAKNHGSSTYIERQFTSITRAPHSFTLQELSETP
                     GTETFWHLNAFGGLQSLPLTSDINYSAKLNYHFFQNKFGIKENRIHLTGNVIMPFHEN
                     NIGLDVQLHQIKYTANEAPLFNFWDYYSVIMLNPFYSIEQTNWKLRMGLKAAFSMKHG
                     RSFSPAADIAAEWNVFPQWMTLYGGLSGDYEINSMDRIFSENCFLFSDTRIKDTYTPF
                     LSYGGVKIKPAKNLLVDFYADYRFTDQQYFYVNKEYITEELNAPDSVLFTNRFNVAYS
                     DAYHFKIGGRAAYNDEKNRFNMEFRTTYNWWDTKDTALPWLQPTWETDLRANLGITSN
                     ITTSLYFFYKTGRYAQLGNRSVKMDNIMDVNLGISYIVSKSLSVYLHLNNLLNNKYQY
                     FYGYDVQGFNLMTGVAFSF"
     gene            complement(19661..22705)
                     /locus_tag="PLD44_02485"
     CDS             complement(19661..22705)
                     /locus_tag="PLD44_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOF98238.1"
                     /translation="MAKKNIPVLTLILFLCLSNLFLSAQHTLFFASEDKDFIQGKELY
                     FQQKYAASYRSFEQFLEKKTFPSDGQFQEAEYFLSANAYELRKENALSLLQTFLTKHP
                     YTPFSEEIYFMIGVLNCEQKNYTVARENFSQVNEQELSENKIPEFLFYKGYTAIQTQH
                     YEEAGKIFSRLKLQNSPFRVDATYYYAYSEYMMGNYQKALPDFLAIENHPDYQISVPY
                     FLVQIYYVEKQYAKLKEKADFLLQKYPNNNNNAEIFRILGELAYGEQNYAQAISYFKK
                     YENLSPKVLRNDVYLLGLSYFQLKDYSNAIQYLSKVTTEPDEISENAYLHLGTSYVFL
                     NDKTNARLSFEAALQTHFNPTVREEALFNYALTCYETTSAFGESVAAFTRFLSEFPNS
                     KYADEAYQYLTSVYLTTKNYELAYQSILKIKKLNTSLTETKQYLLYQMGVNAFAQKDY
                     GKAIEYFSLAHTSAPKGKYSAECLFWRAECYYRTNNPDQAIVDLETFIKNPLSRNSEN
                     LIYAYYSLGYGYFDQKNYIKALEWFSKYLENENSEKSMLYADALTRAGDCHFYARNFK
                     NALSFYEKAAFTSPNTADYSIFQSAYVEGLQKNYLNKIYRLEQLLLKYPDSEYGDDAL
                     YEIGRSYLMLKNEKMALSTYERLMNQYPSSVLSRKAALESGMIYFNRQDFENAIVIFK
                     YVAEKYPDTEESYIALKSMESAYIELNDVNGFLQYTKNSGRIVRPLSVMQEDSISYIA
                     AEKQYINGRYTVAISGFENYLNNFCPGGRYCTIAQFYLADSYYRTQQRDKALKAYEVV
                     LKTNAAEYVEQAALHCAEITYDRKDYASAFNYFKQLKQVAQNSANKQAAHLGMLRCSY
                     FTNDYQSLIDIANVILQDPYSEAIVKTEARFYRAKAYLLHQQLNAAIEDLKIISADTK
                     TAFGAESKYLLASVYFQQEKLTEAENEILDFAKKNTPHQFWLARSFVLLADIYIKKGN
                     DFQAKQYLLSLKRNYTVQDEIQGLIDERLSAISQREKKNIIH"
     gene            complement(22779..23321)
                     /locus_tag="PLD44_02490"
     CDS             complement(22779..23321)
                     /locus_tag="PLD44_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOF98239.1"
                     /translation="MDFFSKFSFCPSCGSADFEINNEKSKRCKACGFVYYLNASAAVA
                     AFILNEKKELLVCRRKKDPAKGTLDLPGGFVDNYETAEEALKREIMEELQLEVENPTY
                     LFSLPNEYEYSNLLIPTLDLFFLCQIKKDLIPIPSDDVEDCFYVPLEKIQLELFGLKS
                     VKKAVEIFLREQIGKKCQNI"
     assembly_gap    23434..23533
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23625..24992
                     /locus_tag="PLD44_02495"
     CDS             23625..24992
                     /locus_tag="PLD44_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOF98240.1"
                     /translation="MGKKLLLPGDDEFKDLVHRYEQYLSGNANGYFDVEEFENIAEYY
                     LLRGRTKDSSRAIDFGMKLHPGSTALKAKRAKIYLAVDEVKKAYQLLSSFTESDYETL
                     LLKIEALVRMGRHAEADKLIKGLTTEKTADLDNIFLDIAYIYLENNKFEKASEFLLKG
                     FQINPKNIDLLFELAFSFEQMEEKEKAIEIYNRILDLDAFSGEAWFNIGQIYFNLQEF
                     PKALEAYDFVLAIDPNDSLAHLQKAHVHYQLEEFYTAIEYYAHYAAMTDDPASTSLYI
                     AECYEKLEQYDKAVDYYKYLLEQDPDNTDLLISIGSCLLEQEKFSASIEYLSRAIELN
                     DSLADAWVYLAEAYLAIDNLDIALKSYLLALQLDSEQPEVIMAVANIYLEKGDYETAL
                     NFYLAVEAMSNSLEIVNVFIAATYYKLGNKTEAYRYLKKGIEDNEDVLNLFLEICPEA
                     IETIH"
     gene            25094..25831
                     /locus_tag="PLD44_02500"
     CDS             25094..25831
                     /locus_tag="PLD44_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOF98241.1"
                     /translation="MRLYGLIGYPLKHSFSKRYFAEKFRREGIDARYDLYELQRIDDF
                     PELVKMPGLCGLNVTIPYKESVIPFLNELDEVAEKIRAVNVIKFLRKNNHLWLKGYNS
                     DIIGFEKAIIPFLKTFHDKALLLGTGGAAKAISFVLAAHNIKVTHVSRKPGINKITYN
                     DLNEELMREHKLIINATPLGTYPNLCSCPDIPYEHLTPQHILFDVVYNPPKTLFLKKG
                     EEQGATIINGEAMLRGQAEAAWEIWNK"
     gene            complement(25979..26803)
                     /locus_tag="PLD44_02505"
     CDS             complement(25979..26803)
                     /locus_tag="PLD44_02505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOF98242.1"
                     /translation="MKKTIISIMFGIMLLLPVFGTAQNNNSNKKSDIQTKSISYKIGD
                     QNFRGYLAWKDDGKKTKPGILVVHEWWGFNEYIKMRTDMLAELGYVAMAVDMYGDGKV
                     YDTAAEAQNAAGYIFSHPELLKERMEKGYEILTSEKTVDKKHVSAIGYCFGGTVVLNA
                     AAMGLPLETVVSFHGGLNGFKASPAMKNTKVLICNGAADMFVTQADIDNFKSEMQKYN
                     ITYEFKNYEGATHAFTNKYSTEAGKKFNMPIAYNEAADKASWQDMILFFNKYFPNK"
     gene            complement(26895..27623)
                     /locus_tag="PLD44_02510"
     CDS             complement(26895..27623)
                     /locus_tag="PLD44_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HOF98243.1"
                     /translation="MENKNNFVIKVEIWSDVVCPFCYIGKHKFEKALEKFPYRENVQV
                     EWKSFQLDPFAVTNPELKTMEHLAAEKGWSMEQALQSTAFITQMAKEVGLEFHFEKAI
                     VVNSFKAHRLLHLAKKHGKQNEMEEKLFAAYFTDGKNIDDNEILLQLAKEVGLEATET
                     NEVLLSDAYSDEVKHDIAHAHQLYVRGVPYFLFDDKFAVSGAQDESVFLQALNRALAE
                     HKKTDIIEFSGENGGVCGPDGCEI"
     gene            complement(27650..28612)
                     /locus_tag="PLD44_02515"
     CDS             complement(27650..28612)
                     /locus_tag="PLD44_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOF98244.1"
                     /translation="MTIIVTFKTFPEQKEYLKNVLGKEMQIYFKEDCTKEELENLMTK
                     ADVLLSWNPVQEGINKQNLPMDNIRFMQLLSAGYDHIRLEDFPEKLQIAANQGAYAEP
                     MAEHVMAMLLALSKRLYVYHNDLAKGNFSQIKSQTKFLKGSVAGIIGFGAIGKATARL
                     LRPFGVKLMATNSSGKTDEEVDFIGTLKDLDYVLQNADSLILSIALNEETEGLINKER
                     LELMKEDAVLVNVARGAIVEEAALYEHLKTHPNFYAGIDAWWVEPFKDRKFELHYPFF
                     ELPNLLGSPHNSAMVHNALLIGTEHAVQKILKFIHGEKVPGLIR"
     gene            complement(28624..29259)
                     /locus_tag="PLD44_02520"
     CDS             complement(28624..29259)
                     /locus_tag="PLD44_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HOF98245.1"
                     /translation="MEQNKITIEFFHDVLCAWCYALSPRVEKLVEKYFDRIEVIHRSF
                     ALAPEPNGIEKIFGSKEEGKIQILGHWRAANMNDDAHRMHPELMATRTFDYPYSTRGL
                     LACKAAELQGGNEMHHKIFNRIQKAHLTECLNINDVEVLKMCAREVGLNVEQWEKDFH
                     SEKVKQMLDEDFYLAYQYGINSVPTLIANGKYKLTGAQPYETLEKWINQIK"
     gene            complement(29267..30085)
                     /locus_tag="PLD44_02525"
     CDS             complement(29267..30085)
                     /locus_tag="PLD44_02525"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HOF98246.1"
                     /translation="MKISEKLPVFFIPHGGGPWHVMDDFMGDPQGYGMLRNYLKKIGN
                     DFHDKIKAILVVSGHWEESVPTVNFGEHPSLLYDYYGFPEFTYQLSWPAPGDPQLALK
                     IENLLLEAGFTTARENLRGFDHGVFVPLMVAFPQADIPVVQLSLIKGLRPAQHIAMGK
                     ALESLRSEGVLIIGSGMSFHNLRGMMSGNQAISKISEQFDKWLTHAVENKNPEIRNQL
                     LTQWLSAPGARESHPRSEHLVPLFVVAGAAGNDLGKQDFSGTLLNVKISSYRFG"
     gene            complement(30133..30693)
                     /locus_tag="PLD44_02530"
     CDS             complement(30133..30693)
                     /locus_tag="PLD44_02530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOF98247.1"
                     /translation="MIKIGIIVGSTRPGRNSEAVAKWVYEVASKRNDAIFEIVDIADY
                     NLPLLDEMIPASMHQYQKEHTKKWAEKINEMDAYVFVTPEYNHAPTAALKNAIDFVYA
                     EWNNKAAGFVSYGSAGGARAVEQLRQIMGEIQIADVRAQVTLSLFTDFENFSVFKPAS
                     SHEGSLHAMLDQLIAWGTALKTVREK"
     gene            complement(30701..30934)
                     /locus_tag="PLD44_02535"
     CDS             complement(30701..30934)
                     /locus_tag="PLD44_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98248.1"
                     /translation="METKKKLRCPLGIPGGIIAALIGLIGIIVNIVAFNWFQLIISLA
                     LLLLALPFVRVTMMVHMANDRLDELEEKINSKK"
     gene            complement(30950..31216)
                     /locus_tag="PLD44_02540"
     CDS             complement(30950..31216)
                     /locus_tag="PLD44_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOF98249.1"
                     /translation="MNKKTTIELFYTLTCPNCKLTKQMLKEVLPQFEDKFSLKTTLAN
                     SPSGMIRTMKLGIHTVPTLLIDNKVVFKSVPTKEELIEKLKNYQ"
     gene            31732..32505
                     /locus_tag="PLD44_02545"
     CDS             31732..32505
                     /locus_tag="PLD44_02545"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOF98250.1"
                     /translation="MDLVGFFILGIAAGILSGLFGIGGGIVIVPSLIVIFGLDILDAN
                     AVSLAAMLLPVGILGVWAYYKEGYVNIGESLWVALGLFLGSFVGGGIAVNIGESLLAK
                     LYAGILIYIAISYFQLPKYFRKKQPIEKEEKPREKKSFWAFVLIGLAAGVFAGLFGKG
                     GGLVIVPMLIHFFHYDSRSAAATSLAALQLPVGLPSVLIYAENGHLNLLFAALIALGI
                     FVGVLIGSKIGIKIPPSIFKKIYAVFLLAIAVYMAFEYI"
     gene            complement(32551..>32650)
                     /locus_tag="PLD44_02550"
     CDS             complement(32551..>32650)
                     /locus_tag="PLD44_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_091910270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOF98251.1"
                     /translation="YFGDAIFDRLLVAAVSYNTDFKSRIYNRNHCG"
BASE COUNT        10287 a         6087 c         5760 g        10416 t
ORIGIN      
        1 tactaaaaac aaaaaatatt tttttgtctt aaacttacca ataaaaaaac ctctgtcgaa
       61 gtagatcccg acagaggtta aaaaaatttt aattctggta ataaaaactt actgctgatt
      121 tttgttctgc aacgtgtaat agattttcaa ctgattggaa agtattttgg taaacccctt
      181 tacatcgtcg gctgtccagc ctttattcac ttcgccatat tctcccatat cggttttcat
      241 catgtcgaag ggactgtcaa cgcctaccaa agtaaagcaa taaggccgaa gtttcaaaat
      301 aacggttccc gtaacgtttt gctgtgaact ttcaagaaat ttttccatgt tgcgcatgac
      361 cggttcgaga tattgggctt cgtgtaaaaa catgccgtac cagtttccca actgctcttt
      421 ccagtattgc tgccatttgg tcaacacatg tttttcgagc aatttatgag cattgataat
      481 aagcatgggt gctgcagctt caaaagctac ccgtcctttg atgccgataa tggtatcgcc
      541 cacatgcata tcgcgaccaa tgccgtattg agaaccaatt ttttcaacca aattgattaa
      601 tgcaatttta tcggaatttt tttggccatt cactccgcaa atttctccct cttcaaattc
      661 gatagtgaga atttcttcgc cctgtttttc cactttgctc ggataagctt cttccggcag
      721 cgtttgatcc gatttcagag tctcctttcc gccaatgctt gtgccccaca atcctttgtt
      781 gatggaatat tccatttttt tgaaatcggc ttcaaatccg tgttgtctta aataattgat
      841 ttcgtattcg cgactcaagg caagatcacg aataggtgtc agaattttta tttccggtgc
      901 cagtacactg aaagtcaaat cgaaacgaac ctgatcgttg ccggctcctg tactgccatg
      961 tgccacgtaa ttggctccaa tttctttggc ataattaatt atggctatgg cctgaaaagt
     1021 acgctccgaa ctcactgaaa ttggataggt tccatttctt aacacattgc cgaaaaccat
     1081 gtatcgaata catttttcgt aataatcttt tgttacatcg agcaccacat gtttcactgc
     1141 tcccagttga taagctctct tctcgatgtt ttgcaaatcg gcatcactaa atccccctgt
     1201 attggcaaca gcggtatata catcaaatcc tttttcttcc gaaagatact ttacgcaaaa
     1261 cgaagtatcc aatccaccac taaaggctaa aacaactttt tctttcattt tttttttgaa
     1321 tatcttattt ttctattgat tttctgattt ttttcttttt taaacgggat ttaaaaccca
     1381 tttaattcga ttttaaaagg gattattttt cttctttttc cgatgtcggg gttgaagatt
     1441 ttttttgttc agcagcaggg tcaaacaaca tgccggtaca aaggcaatat tttcgtccgg
     1501 ttcgttgcag aatatcatag tttacacaac tttggcaccc ggcccaaaaa gcttcatcat
     1561 tggtaagttc agagaaagtt accggtttgt aacccaattc ggaatttatt ttcatcacag
     1621 ccaatcctgt agtaagacca aaaatcttgg cgtcaggata acgttcgcgc gaaagttcaa
     1681 aagctttctg cttaattctt ttggccaatc catgtcctct gaatttttct accacaatca
     1741 aaccggaatt ggctacaaat tttttgtcgc tccaagtttc aatgtagcaa aaaccggcaa
     1801 actcttctcc atgaagagca ataatggctt ttccttcctt cattttttgc tcaatatatt
     1861 cgggcgaacg acgtgcaatg cctgttcctc tgatttttgc tgcttcttcg atggttttca
     1921 atatggtatc cacatatttg atgtaacttt catcggcaac ctgaacggta atctcatcca
     1981 tgctaaagtt attttccata atttttttgg attcctccta tcttcttcat ttataaattt
     2041 ttatctacga atttcatcta tcaaaaaaat caaactgctt ttggctcgcc aaaaatatcg
     2101 ttcataatct ctacgactac ttcccgctcc actccttcac gcggcactac caaaatggta
     2161 tcgtcgccgg ctatggttcc caaaatgtaa tccgatgctt tgttgtcaat atcccaagct
     2221 atcgcgctgg catatcccgg tttcgtcttc aacacggcca gttgctgcga aaattccaaa
     2281 cttagaatca ctccattggc caacgaacca ttcagcgtat tggccgaagg tggtggcagg
     2341 ggcttgttgt acgaaggcaa cacgtatttg tatgtcccat tggaaagcgg agttttggcc
     2401 actttcaaca ctttcaggtc gcgcgacaga gttgcttgcg taatttcgca ctgctgttcc
     2461 ttcagtaatt tcaacaattc ttcctggctg ccaaccacat ttgaacgaag aatgtcggaa
     2521 ataattttta agcggtttcg tttgtttttc ataaaatttt aatatataag tatttgtttg
     2581 taattacttt tctgatgaaa cccacatgta tttcatacac aaacacaaat gaatataata
     2641 ttcagacatg tgggttccat cagaccttta aaaaataaat tatattctga tgaaaacgaa
     2701 tgtcttatat gaaaccaaac tgattctcta ctcaaatatt tttagtatat ttataagaat
     2761 attttgcaaa tatacgaaat ttattccaaa atcacgaata aataactaat aaatatcctt
     2821 attaatgcca ttatttaaca attattcgat taaacaggta tatttatgca ggaaaaatct
     2881 aaataattac tcaaaacata aaagaatatt aaatgaataa aaagtgggta acactatcag
     2941 tattatgttt tttgaagtat tacaggaaaa ttgaaaatta tcttgtaaat ttgcaggata
     3001 taattcatcc ataataaagg ttgatatgct gcaggataaa aaaatcattt tgggcattac
     3061 gggtggaatt gctgcttata agtctgccac gatagtccgt ttactgttga agcagggagc
     3121 tgaagtaaaa gtggttatgt cggcttcggc aaaagaattt attacaccct tgacacttgc
     3181 tgcactttcc aaaaatccgg tattgaccga ttttttcgat ccaacaaatg gtcaatggaa
     3241 cagccatgtg gatttgggct tgtgggctga tgcatattta attgcaccgg caacagccaa
     3301 ttcattggct aaaatggcag ccggcattgc tgataatttg cttttgacta cttatctttc
     3361 ggccaagtgt cctgtttttt tagctccagc aatggatttg gatatgtttg ctcatcctgc
     3421 tacacagcgc aacttgcaaa ttcttcgttc gtatggaaac ctcgttatag aaccttcatt
     3481 tggtgaattg gcaagtggtt tggaagggaa ggggagaatg gaagaacccg aaaatattgt
     3541 tcattttctt gacgattatt ttacacccaa acaacttgcg gggaaaaaaa ttctgattac
     3601 tgccggtcct acctatgaaa aaatagatcc ggttcgtttt attggaaatt tctcatccgg
     3661 aaaaatgggt tttgcgttgg ctgaatcatg tgctgctcat ggagctgatg tttgtctgat
     3721 tgcagggcct gttcaattaa gtttgcaaca taaaaatatt caactcatta aagtttcatc
     3781 cgctcaggaa atgttcgacg ctgttacgga aaagtttccc gaaatggatg gtaccatatt
     3841 aagtgctgca gtagcagatt ttacaccggt ggctattgcc gaagacaaga taaaacgtaa
     3901 gggcgatcgg ttgaccatag aactaaaacc aactccggat attgctgcta cactcggcaa
     3961 aataaaaacc gaaaaacaat ttttagtagg ttttgctctc gaaacttcca atgaaattga
     4021 aaatgcacaa ttgaagttga aaaataaaaa tttggatttt attgttctta attctcttcg
     4081 tgacgaaaaa gccggatttg gctacgatac caataaaatt accattttgc ataataccgg
     4141 caaaagcgtt tcctttgatt taaagccaaa aaaagaagtg gcggaagata tagttaatga
     4201 gttggttaaa attttgaatc gatgaaaaaa aagtttgcta ttctgatttt cttatcattt
     4261 tgtttttcct ttcatgctta ttctcaggaa ttaaactgca atattagcat taattccgat
     4321 caggtgcaag gaacaaataa atctgttttc aacactttgc agaaatcggt ttccgaattt
     4381 gtgaacaacc gcaagtggac ggaaatgact tttttaacca acgaacgaat tgaatgttca
     4441 atgaatatca ttgtaaaatc ggttgatgga gataatttta ctgctgaaat tcaggtacag
     4501 tcgcgtcggc cggtttacaa ttcgaattac aacacaactc ttttcaattt caaagacaac
     4561 gattttacgt tctcatacaa ggagtttgag ccgttggaat ggaacgaggc atccatcagc
     4621 tccaatctca ctgcggtatt ggcttattat gcctatatca ttatcggcta cgacatggat
     4681 tctttttcaa ggttgggagg cacaccattt tttcaagccg ccgaaaatat agttaattct
     4741 gctcagggaa gtaatttacc cggatggcga gcttttgaaa gcagccgaaa tcgttatgct
     4801 ttggcaaata atttgactga tgaagctttc aaaaattttc ggaattattt ctacgaatat
     4861 catcgtctgg gattggacga aatggccaat aatccagtta atgcccgtgc ccgaattgct
     4921 caaggaattt ccgtgttgcg cgaagccaat cgtgcccgcc catcggcagt aataatttcc
     4981 acatttttgg atgccaaaac ggatgaattg atcaatattt tttctaaagg aactgacaag
     5041 gaaaaagcat tggtcgtaga aattctgtcg gatgtaaacc ccacacagac acaaaaatac
     5101 gaacaaattt taaataaaaa ataaatactt tttctgacta ccacatattt atgcttaaga
     5161 cgcttcatat cagcaattac gcattgattt ccgaaattaa tatcggtttt aattccggct
     5221 tttcagttat tacaggcgaa accggtgctg gaaaatccat cataatcggt gcgctttcgt
     5281 taatactcgg acagcgggct gaaagtcgtt ttatcaagga aggagaagaa aaagctatag
     5341 ttgaagccga atttgatatt cgggattatc gtttggaaca tttttttgaa ataaacgaat
     5401 tggattttca tcctgagcag tgcattatcc gtcgtgaaat aacagcatcg ggaaaatcaa
     5461 gagcttttat caatgatacg cctgtaagtt tacagctttt acgtgaactc agcgccaaat
     5521 tgatcgatat acattcacaa cacgaaaatt tattgctttc agacgaccag tatcaattgc
     5581 aagtggttga caacatggca caaaatgctt cgattctcaa gcaatatcaa caatactata
     5641 gtcaatggaa aaacctgcaa acacagcttt cccaattgca gaaaactgcc gataaacaat
     5701 tgtctgagct cgattattta aaatttcagt ttcaacagtt ggaagaagcc catttgtcgg
     5761 aaaacgagca agatgacctt gaaatggaac tggaaacatt gagccacacg gaagaaatta
     5821 aacaggaatt gcaaaaagcc gtttttttac ttaccgatga acaaaaaagt cttgttttgc
     5881 ttaaagaagt tatcgacgct gtaggacgaa taaaaagtta catttcggaa ggagagaatt
     5941 ggcataaacg tttagagtcg gtttatatcg aattgaaaga tatagctcag gaaataacaa
     6001 gtttagagga aaatattgat tttcaccctg atagattgga atgggtcgac caaagattga
     6061 atgaaattta tacgttggaa aagaaattta aactgagttc tatttccgaa ttattatcgc
     6121 tgaaagaaaa tttaaaagaa gaattgcagc acatcgaatc ttttgatgaa gaaatcgacg
     6181 ccttgaagaa aaaaattgcc gaagctcata aaaatgttca tcaacaagcc gaattattga
     6241 caaaaagtcg gttgaatgcc gttgagccga tagaaaaatt tttgaaagag cagctttcac
     6301 gtttgggaat gccgaacatt cagttcaagg ttgaaattac ggatgctgcc gaattttcgg
     6361 aaacagggaa agacacggtt caatttcttt tttcggccaa taaaaatcaa tcgttgcagc
     6421 ctgttgctca aatcgcttcg ggaggtgagg tttcacgttt aatgctctcc atcaaatctt
     6481 taatggcaca tcgagccggt ttgcctacca ttatttttga cgaaatcgat acgggcgttt
     6541 ccggcgaaat tgctcatcga atggctgaaa ttatgaaaga aatgagtaaa aacatgcagg
     6601 taattgctat cacacattta cctcaaattg ctgccaaagg gaaccaacat tatcgtgttt
     6661 ttaaagacga ttcaggcaaa actacacaga ctcatatcaa cttgctcgat agggaagaac
     6721 gaatttggga agttgcgcaa atgctcagcg gtgaaaatgt tacggaagtt gcattgggca
     6781 acgccaaaga attgcttaaa agcgaataaa aaaccggaaa tctgaattac ggtaacataa
     6841 acaattgcaa aaatcacttt ttacaaaata ttatgataca atccatgaca ggtttcggca
     6901 aagctacctg tgaatatgaa gggaaaaaaa ttgtcgttga aattaaatca ctcaacagca
     6961 aacaactgga tctttctttg aaaatagcgg ctgcataccg aaataaagaa ctcgaaatca
     7021 gaaccgaaat tcttcaaaaa ttggaacgcg gaaaaattga tgtggctatt tacatagata
     7081 actcaggcaa agatgttgct aatcaaatca atccatctgt ttttttatct tacatgcagc
     7141 aaatcaaatc tttatcacag aatcttgaat tgcctgagcc aacaaattgg tttgatattt
     7201 tgcttcgatt gccggatgta atgaaaaccg ataacatcga attggaagaa aatgaatggc
     7261 aagcacttaa gaagagtatt gacaaagcga tagaacaggt tatttctttc cgtattcagg
     7321 agggaaaatc tctggaaaac gtttttatct caaaaattac aaatattggc gatttattgg
     7381 aaaaaatttc gccttacgaa gcggaaagaa tagaaaaaat acgttccaga ctcaaagaca
     7441 atctgcaagc actttctgat aaggtagatt atgatcaaaa tcgtttagag caggaattga
     7501 tttattacat tgagaaactc gatattaatg aagaaaaagt tcgtttgcga aaccatctga
     7561 attatttctt tgaaaccatg aaactggaac catcggcggg caagaaactc ggttttattg
     7621 ctcaggaaat tggtcgtgaa atcaatacgc ttggagccaa agccaatcac agtgaaattc
     7681 aaaaaattgt ggtgaaaatg aaagacgaac tggaacaaat gaaggaacaa gtattgaatg
     7741 tcctttaatt tgataaattt cttttattat aaggtaaaac aattgtacta cgtttaaaat
     7801 ttcactttct tcatgtccgg aaaattaatt attttatccg caccatcagg cacaggcaaa
     7861 agcacgctgg ttcaatacct tctgacacgt ccttttgatc tggaattttc agtgtcagct
     7921 accagtcgtc caccgcgcgg aagcgaacag aacgggaaag attattattt tctttctaaa
     7981 gaagaatttc agaaaaaaat agcgcaaaac gaatttttgg aatacgaaga agtttatccg
     8041 ggatgttatt atggaacttt gcgaagcgaa attgatcgca ttgcagcgaa ggggaaaaat
     8101 atcattttcg atgtggatgt gcttggagga cttaatatta aaaaacaata caatggcagg
     8161 gcattggcca tttttattgc tcccccaagt ttggaagaat taaaaaatcg tctgacgatt
     8221 cgaggtaccg atcctcccga aatgattgcc aaacgacttg aaaaagctga atttgaaatg
     8281 agtttcgctc caaaatttga tgtagtaatt gtgaatgacg atttggaaaa agctaaaaaa
     8341 gcaaccgaag aagttgttcg caactttttg gaaaaataag ttgtcccgca aaatgaaaaa
     8401 agttggatta tattttggtt ctttcaatcc ttttcatctc ggacaccaaa aactggccga
     8461 atttcttctt gatcatcaag tggttgatga agtttggttt gttgtttctc ctcgcaatcc
     8521 gttaaaaaag gaaaatgatt tgctggacga aaatcttcgc ttggaaatgg taaaaatagc
     8581 cattcaggac aacccgaaat taaaagtttc cgacctcgaa ttttctatgc ctaaacctgc
     8641 atataccatt gacagtctgc atgctttttc ttccaaatac cctgaaaacg atttttacct
     8701 gctcattggt agcgataacg caaaagtctt tgatcagtgg aaaaacttta agcaaattct
     8761 cgatgaatat cccgtgttgg tttatccgcg tcccggattc gataaagaag aaattgccaa
     8821 aatctatcct caaatgaaat ggttggaagg tccacaatac aatatttctt caacggaaat
     8881 tcgccaaaac attaaacaag gtaaagaagt aaaagatttg cttcaacctg ctgtttatca
     8941 atatattttt aaaaaaaagc tatatgttta atgctaattt ttctctccca aaatttcttt
     9001 tatttcatta taactcaaaa cttctttttc taaaagtgtg atggccattt tctccaataa
     9061 atcttttttt tctgacaata atttttttgc attttcataa catgagcgaa tgatttcggt
     9121 catttcttca tcaatcatct tttcaacata ttccgaacgt cgttcgatac cgggtccgcc
     9181 acccaaaaaa ccggtagttg actgatttac gagggaaata ttaggcaatt ttttactcat
     9241 tccataaact gtaatcatgt ttcgagccaa ttgagtagct ctttccaaat cgttggaagc
     9301 gccggttgac acttcaccaa aaatcaattc ttcagcagct cttccgccca aaagtccttt
     9361 tatttttcct atcaattcac tttcagagag tagatatctg tcttccagag gcatttgcat
     9421 ggtatatccc aaagctccta tacctcttgg aactattgaa actttctgaa cctcgtctgc
     9481 tcccggagtg aagtatccga caatagcatg gccactttca tgataagcta caatttttcg
     9541 ttccttttcg tttataagtt tgttcttttt ctccaaaccg gcaattacac gctcaatagc
     9601 ggcttcaaaa tccatcatgg taacttcatc acgattgttt cgtacagcta aaattgcggc
     9661 ttcattacaa acgttggcaa tttcggcgcc tgcaaatccg ggcgtttgtg cagctaatct
     9721 ctttaaatcg acatccgaag ctaatttaat attttttgta tgtactttaa aaattgcttc
     9781 tctcccattc atgtcgggtt tatcgaccaa tacctgtcga tcaaaacgtc ctgggcgcaa
     9841 caacgccgga tccagcacat ctggccgatt tgtggctccc atgataatta ctccggaact
     9901 ggcatcaaat ccatccattt caaccaaaag ttgattcagc gtattttccc gttcatcata
     9961 accgcccgca taatatcccg agtgagctct gcttttaccg atagcgtcaa tttcatcaat
    10021 aaaaataata cagggtgcat gagcctttgc ctgattgaac aaatctctaa ctcgggcagc
    10081 cccaacccct acaaacattt cgacaaaatc ggaaccgctc agactaaaaa atggaacatc
    10141 cgcttcgcct gctactgctt tagccaacaa ggtttttcct gttcccggag ggccaaccag
    10201 caggactcct tttggtaatt ttccgcccaa acgtgtatat tttgaagggt ttttcagaaa
    10261 atcaaccaac tcttttacct cttcaacggc ttcgtcaatc cctgctacgt ctttaaaagt
    10321 tattggattt gcaggttttt ctgcctgaat ttgtgctttg tttcttccaa tttcgagcat
    10381 ggggtttttg ttgcccattt ttctgaaaaa atatccccat aacagcataa acaacacaaa
    10441 tggtaaaatc cagttgagaa aaaagtttaa aacagtattg tcttgaaaat gacctttaaa
    10501 attaactttt gccgccaaca aatcattaat gagatattct tcgttcatat ttggaattct
    10561 gctgacaaca aattgctttt gtacttgttc ttccaaatca ggaattctga ttctccacgg
    10621 ggcagtggga gcttgtgtca ttgaggaaat atggttggta tccgatttga attctccaac
    10681 tattttatcc tgtaaaaaaa taattctctc cactttgccg tttcgtacag aatcaacaaa
    10741 ctcgttatag cttaattccc tgttcccttg tgtggtcgga aaaataagcc aatccatcaa
    10801 aataaacaaa aacatgataa ccagccagta aatccatgaa tattttgcac gttttgtcat
    10861 tcaaataaat ttttttaggt ttataaaatt tcgacgaaaa ttagaaaaaa aatcgggaaa
    10921 ctcaatgaaa aaccgatttt attatttgat ttttttacgt ttattatttc caaaacacaa
    10981 aaaatcccga ctttttaaat ttttgagccg ggatttttaa aataattcgt taaaaaacaa
    11041 tcacaaaagg ctccatgcta cagtgagacc tgccattacg atggaaacca tgctcatgag
    11101 ttttattaaa atattcaagg aagggcctga agtatcctta aaaggatctc caactgtatc
    11161 accaaccact gcggctttgt gattttccga acctttaccg cccaaatttc cttcttctat
    11221 atattttttg gcattgtccc atgcaccacc ggaattggcc ataaaaattg ccagaatgaa
    11281 accggtagaa acacttccca gcaacaatcc catgacaccc gaaacgccaa atatcacgcc
    11341 ggtaagaata ggaataatga ttgctaacaa ggaaggaagc aacatttcct tttgcgctcc
    11401 ttttgtagag atggctacac atcgagcata gtcgggttca gctttgcctt ccatgatacc
    11461 ggcaatttca cgaaattgcc gacgaacttc ctgaaccata ttttgagcag cacgtccgac
    11521 tgcattcatt gtcaagccac aaaacaaaaa agcagccatt gcaccaataa atgctccaac
    11581 taaaactcgt gggttcatca aattaatttg gaaatattct attaattgag gaatagaagc
    11641 ctgatgaata tcggtctgca tggcattgaa aaataattct gttttttcgg gaatacgagc
    11701 aatggcaatt ttgatttctt ccaaataaga agccatcaat gccaaagccg taagggcggc
    11761 agaaccaatg gcaaatccct ttcctgtggc agctgtagta tttcccaaag cgtccagtgc
    11821 atccgtgcgt cttctaactt caggagccaa tccgctcatt tctgcatttc ctccggcatt
    11881 atcggcaata gggccataag catcagaagc tagcgtgatt cccaatgtag aaagcattcc
    11941 tacagccgaa atggcaattc catacaatcc catgctgata ttggaaaaat cgaaacccga
    12001 agcaaacaga taggacaaaa ttatagaaat acccaccacc aaaaccggaa aagcagtcga
    12061 aatcattcct gttccgatac cggcaataat taccgtagca ggtccggttt ttgacgattc
    12121 agaaattcgc tgtgttggtt taaaacctgc cgaagtgaaa tattcggtta tttttccaat
    12181 ggcaacacct ccaaaaagtc cagccagcat cgatccccaa atgcccaaat aattaggtat
    12241 attcaaatat ttcaaaataa gaaatgaaaa tatggcaatg aaaatggcgc tgaaattgat
    12301 tccacgactt aaagagctta acaattgctt ttgagaagca tcttctttgg attttaccat
    12361 aaatattcca ataattgata gaataattcc aacggctgca ataatcatgg gcgcaattac
    12421 gccattaaat tgaactgctg ttccttttgc cgcaaaagcc acagctccta atgcagcagt
    12481 agaaagtatc gatccacaat atgattcgta caaatcagcc cccataccgg ctacatcgcc
    12541 tacattatca cccacattat cggcaatggt ggcaggattt cgaggatcgt cttccggaat
    12601 attttcttcc acttttccca ccaaatcggc tcccacatca gcagctttag taaaaattcc
    12661 acccccaaca cgcgcaaaca aagcctgtgt agaagctccc attccaaaag ttaaagtagt
    12721 agtagtgata ataatcagtt tcatggaaga atcggaagct atagtcaaat tagtagcatt
    12781 atccaaagct ccaataacaa actgcaaaac ataaaaccaa atggaaatat ccaataatcc
    12841 gagccctaca acaaccaatc ccataacggc tccggaacga aatgcaagtc tcagtccggc
    12901 attcaaactt tgctgagcag cattagcagt acgagcagaa gcataagtag ctgttcgcat
    12961 tcctataaat ccggctaagc ccgaaaaaaa tcctcctgtc agaaaagcaa atggcaccca
    13021 cggattttgt attttcaaat aataggccat aaaggaaaaa attatagtta ttataagaaa
    13081 tacaatgcct actactttgt attgttgttt caaataggcc attgcacctt ccctgacata
    13141 ttttgctatt ttttgcatac taatggtacc ttcactttct tttttcatct gatgaaaaaa
    13201 ataccacgca aaaaataatg ccaatacagc agaaacaggt accaaaaaga aaactttatt
    13261 ctccataacg tattgatttt aagtatatta attaaaattt caaaatttat tcattttgat
    13321 aaaaaattca aatttaaaat tattgagcac ccgataactt tgttagcctt tgatataaag
    13381 taggatgtga ataatgaaaa aaagcataca agggatgagg catcaaattg ctaagtgaag
    13441 tggcagccag cttttttaaa ccactctgca gctgctttgc ccagccaaaa tttgcagcaa
    13501 aagcatctgc ctgatattcg aattttctgg ataaaatatt ggtacaaata ctcaacaaca
    13561 gcgaaacagg tgtatataaa aggccaaaag caatgacgtt caaatgaaag gaagcagttg
    13621 ttccacccaa aacttgagca aaaacatcac ttttcagtat caatcccaat aggaaaaata
    13681 gtaaaagact ttccggaaga gaaatcagaa attgataaag tgtgtgtttc tttttataat
    13741 gaccaatttc atgcgccaaa acagctacta tttcttcaga agtaagcttg tcgagcaaag
    13801 tatcgtaaag cactactctt tttttgtttc ccaaaccggt gaaataagca tttgcttttg
    13861 tcgaacgttt tgaactgtcg ataatataaa tattgtccaa tttgaatccg gctttggttg
    13921 caaatttttc tatctcatcg cgtaattctc cttcttcaag cggcttttgc ttgttgaaaa
    13981 taggtacaat gatttgtgaa taaaacatgg acatgaacaa actaaacaag gtaacaaccg
    14041 accaagcaat caaccagaaa tactttggag ttaattgata tatccaaact attgcgccta
    14101 ataaaatgcc tcccaaaaat acacttaaag caaattcctt taaatgatcc tgaacaaaga
    14161 gctttggtgt taccttatta aatccgaatt tctgttctat aacaaaagta tcgtaccact
    14221 gaaaaggaat actgataagt gtttctgcaa caaaaagcac tccgaaatac aacaacggca
    14281 ataaaatggg atgctcggtt attcggcgca gaagcgtatc tagccaacca aatccgccta
    14341 aacagaaaaa aattaatatt aaaataaagt taatggtgga agaaatggtt ccgaaacgta
    14401 tattggatcg aaaatattct tgctgtttac cgtatttgtc ggcgtcataa atatctttta
    14461 aattatcggg aagttcattt ttggaatatt gaaaattcaa aaaagacaaa taacgctcca
    14521 aaataaaatc acctactata aaaaataaaa tcaaataaaa caaaatttct accagtgaca
    14581 taatctttcg tttcaattat ttaataaatt gagtgcaaat ataattaatt taatgatttc
    14641 ccaacccttt gggctcaaaa tttacataaa aagcatatct ttgcctttcc tttataaaaa
    14701 tttcaatttg tatgctgtca gtcatggaaa tccttaaata tatgaagaga ttacaaatat
    14761 ttttatttat tctcataact cttttttccc tttcctgttc caataaaaca gcttccgata
    14821 aaatcagcat tgccatcccg gaaggtcctt cagttgtcag ttttataaaa atgatggacg
    14881 attccgtgtc aattgaaggg aaaaaagtgg aatatattgt gaaatccgac ccacagcaaa
    14941 ttcaagccct catgatgcaa aacaaagtgg actttgccat tttaccaacc aatatggcgg
    15001 ccaatctttt caacaagggc attaaatata aaatgatagc ctgccccgtg tggggaactt
    15061 tgtatatttt gtcgaacttg cctgaagtaa aaactttaaa cgatttgcaa aacagaaaag
    15121 taagcatttt tggtcagggc actacagccg atatcctgtt tcaaaaattg atccagaaaa
    15181 aaggaataga aaaagttttt attgattatc gttttacggg aaattttgaa ctttcccaag
    15241 cgcttcgttt aaagaaaacc aatatagccg ttatttccga acctttggta agtaaacttt
    15301 tggaacagga ttctaccatt catatcgtag aaaaattgtc atgcgaagag tatctaaatg
    15361 aaaaagatag agatatattc gtacaaactg cttttttagt caatgaaaaa ttggaacgga
    15421 ataatcctca gattataaag gaaatatgta atgcttatcg tgccagttgc aattatgtta
    15481 ctcaacatcc ggaagaagct gccgttttac tggttaaaca tggcatttta ccggaaacaa
    15541 atgtggccaa aaagtcaatt ttgctgtgca gtatcaatta cgtagctgcc tttgccatcg
    15601 aggatgaaat ttacagttat ctgaaaattt tttacgactt caatcctgaa agcattggag
    15661 gaaaattacc ttcagaaaat ttcatttttc aagtttttta gccttaacgg atatgaaaaa
    15721 tcagaataaa aaaatttttc cttttattct ttctgtgttt tcgctggcaa ttatttggca
    15781 aattatttct atatctattg gttatcctgc tatttttccc tccatttttg atttaatatc
    15841 agatacgtta catttattta cccgctccga attttatttt gcattattgg gaaccgtcag
    15901 cagagggtta ttgggttttg ttatcacttt ctttattgct ctcttttttg gaagtttaag
    15961 tgttttcaat tcatttttca aggatttttt tcatcccatt gtggttttta cgcgctccat
    16021 accggtgata tctatcgtgc ttgtcgctct tttgtggttt tctccccctt ttttacctgt
    16081 ttttgtagca tttttcagca tgtttcccat tctgtttcaa agtatccaaa gcggattgga
    16141 aaatgtggat aaaaaatggg tcgaaatggg caaggttttt ggactttcat ctcgaaagcg
    16201 ttttttacac atttattatc ccgctgccag atcgactatt tttgacggca tcagttctgc
    16261 aatgggattt ggatggagag ccgtaattat aggcgaagca ctggctcaac ctgttcatgg
    16321 cattggttcg caaatgaaag ctgctcaggc ttatattaat gtttccgaaa ttatagcatg
    16381 gacacttgca gccattttga taagttatat tttcgagaag ataattcttt ttctgaaaaa
    16441 gtgtaatcgt cccattcaac tacccaaatc agaagaatgt aagttgcata aattcaatca
    16501 aatagttgat aaaaaggaaa ttactattta taacttaaag aaaaaattta atgattatac
    16561 tgttttttcg tctttcaatg aaaactttaa ggacaccgaa attaatttta tcaaagggcc
    16621 atcgggcatt ggaaaaacaa ctttattgag gttgatagca cgacttgaaa aacccgacga
    16681 aggaacgatt tattattctt caaaattcag ttttggatat tcgtttcaag atgttcgttt
    16741 attgccttgg cttactgtta aagaaaatat cgcatttcca ctgttgtata aaaaaacaga
    16801 tagacaagaa ctcaccgata acatcgattg gctgctaaat aatatggagt tgacagaaca
    16861 tcaaaacaag tttcctgatc aattgagtgg aggacaattg caacgcgtcg gattggctcg
    16921 cgctttagtt gcgcaacccg acgttttgct tttggacgaa ccgtttacag gttttgacga
    16981 cgatttaaaa acaagaattc aagatttctt atcgaaatgg tttgaagatt ttcagccact
    17041 cgttatttgg gcaacacatg aaaatattct ctttccggaa aaaaatagaa gagaaattca
    17101 acttaaaaat tgaaacgatg gatatctgct gaaaacatcg aatttggaaa ttaaacaaaa
    17161 gcaacaattt tgttgggata gttgttaaat ttacgttgat ttgactgatg taaataattg
    17221 ctacagctcc aataatcctt cgggcaaatc catgtaaata atttttttca tttcatcaat
    17281 ttgtagaatg taatcttggc aagctggaat cagtatctgc ttttcgccat tgtcaactat
    17341 aaaaaggacg ttttgtgtac tggtatcgat atctgtaacg ataccgatga aattattgtc
    17401 tttatcgaat attcgaaatt cgataaatgt attgttatcc atatcaacat cactttccaa
    17461 gatattcaaa aattttttat gcaaataaat agtcaatcct aaaaaatttt ttgctttttt
    17521 ttcattgtta acatcttcaa tttttaaaaa gccgctattt tcagaattga aacgccattc
    17581 tttcaaataa aatggaacaa ggatgccatg catatcgaaa atcaaaaatg ggatattatc
    17641 aaaatccaat ccttcgtaat tggacatgaa agccatttcc ccgttaattc cgtgtaattt
    17701 aattatttgt cctatgggta taacttctgt aaaagaaatc atctcagaaa aattttaaaa
    17761 atccggaaaa aaattttttt caacaaatca agccattttc cgaaaaatcg tcaaaatgaa
    17821 aaggctacac ccgtcatgag gttgaatcct tgaacatcgt agccataaaa atactgatat
    17881 ttgttgttca gaagattatt caagtgtaaa tagactgaaa gcgacttgct gacaatatag
    17941 gaaattccca agttaacgtc cattatgttg tccattttca ctgaacgatt gcctaattga
    18001 gcatatctgc cggttttgta aaagaaataa agggatgttg tgatatttga agttattccc
    18061 agattggctc tcaaatcagt ttcccatgtc ggctgtaacc agggcaatgc cgtgtctttt
    18121 gtatcccacc aattataagt ggttcgaaat tccatgttaa aacggttctt ttcgtcattg
    18181 tatgccgccc tgcctccaat tttaaaatga taggcatcgc tgtaagcaac attgaaccga
    18241 ttggtaaata aaaccgaatc gggagcattc aattcttcgg taatgtattc cttgtttaca
    18301 taaaaatatt gctgatcagt aaatcgataa tcggcataaa aatcgacgag taaattttta
    18361 gcaggtttta ttttgacacc tccatagctg agaaaagggg tgtaagtgtc ttttattcgt
    18421 gtgtcggaaa ataggaagca attttccgaa aaaattctgt ccatgctatt gatttcataa
    18481 tcgcccgaaa gaccaccata caaagtcatc cactgaggaa aaacattcca ctcggcagca
    18541 atatcggctg caggactgaa agaacgccca tgtttcattg aaaaagcggc tttcagtccc
    18601 attcgtaatt tccaattggt ttgttctatg gaatagaagg gattaagcat aataacggaa
    18661 taataatccc aaaagttgaa tagaggagct tcattagcgg tatattttat ttgatgaagt
    18721 tggacatcca agccaatatt gttttcgtga aaaggcataa taacattgcc tgtaagatgt
    18781 attcggtttt ctttgatacc aaatttattt tgaaagaaat gataattcaa tttggcagaa
    18841 taattgatat cggaagttaa aggtaatgac tgcaaacctc cgaatgcatt gagatgccag
    18901 aaagtttccg taccgggagt ttcggacaat tcttgtaagg taaaagaatg aggcgcacgg
    18961 gtgatggagg taaattgccg ttcaatatag gtggatgaac catgattttt tgctaattca
    19021 tttagttgaa cagaggcgga atcgttaaaa ttaaagccat aaaatttcaa tctttcatgt
    19081 tgagtttcta ctccggcaaa caaatgtaag cttttcaatt gttggtcaaa aaccaatccc
    19141 gatttggttg ttgaatgaag tttgtcgccg aaagttccaa tatgattcat gtaaaagtcg
    19201 agctttgtat tgggacgatt aagtaaagaa tataaaaaat ttgcggaaga attatagaag
    19261 tttcccaatc ccaactgtaa aaaattgttt tgttctaccc tgttattaaa aaaaaactca
    19321 gttgacgaca acggatgaat attgttatta atggaaaggg gaataaaaag atcagtatat
    19381 tgagccggta gaggttttgc ttcgggttca ataagtttgg gtgtaacatt taattttccg
    19441 gtatttttta gtataggttg atattccctt tcaactgtaa tgtttcgggt gaaaacagaa
    19501 tcctgagcca aagatatggc aaaatttaca cagctgaata tagataaaaa tactaatttt
    19561 ttcttcaagt ttatcatatc aatttacggt ttgtgaaaat aaatacaatt ttgtctaaaa
    19621 aaaaaacatt tttgtcaatc aaagagttat tcctaaaatg tcaatgaata atatttttct
    19681 tttctctttg gctaatagct gacaatcgct catcaatcaa accctgaatt tcatcctgaa
    19741 cggtataatt tctttttaag cttaaaaggt attgttttgc ttgaaaatca ttcccttttt
    19801 ttatgtatat gtctgccagc agtacaaaac tacgagccaa ccaaaattga tgaggcgtat
    19861 ttttttttgc aaaatccaaa atttcatttt ctgcttcagt cagtttttct tgttgaaaat
    19921 ataccgaagc taacaagtat tttgattctg ctccaaaagc tgttttggta tcagcagaaa
    19981 taattttcag gtcttcaatg gctgcattta gctgttgatg aagaagatat gctttagcac
    20041 ggtaaaacct tgcttcggtc tttacaatag cttccgaata tggatcttgc aaaattacat
    20101 tggcaatatc gataagactt tgataatcat tagtaaaata actacaacgt aacattccca
    20161 aatgagcagc ctgtttgtta gccgaattct gagctacttg tttaagttgt ttgaaataat
    20221 tgaaggcaga tgcatagtct ttccggtcat aggtaatttc tgcacaatgc agggcagctt
    20281 gttcaacata ttcggcagca ttggttttca gtacaacttc ataggctttc agagccttat
    20341 ctctttgctg tgtacgataa tagctgtcag ccagataaaa ttgagcaatg gtacaatacc
    20401 tgcctccggg acaaaaatta ttcaaataat tttcaaaacc tgaaatagca acagtatatc
    20461 taccatttat gtattgtttt tcggctgcaa tgtaagaaat ggaatcttct tgcataaccg
    20521 ataagggacg aactatacgc cccgaatttt tggtatattg caaaaaacca ttcacatcgt
    20581 ttaattctat atacgcactt tccatgcttt taagagcgat atatgattct tcggtgtcgg
    20641 gatatttttc cgctacatac ttgaaaataa caatcgcatt ttcaaaatct tgtcggttga
    20701 aataaatcat accactttca agtgctgctt ttctggataa tacagatgaa ggatactgat
    20761 tcattaatcg ctcataagta gacaaagcca ttttttcgtt tttaagcatc aaataagaac
    20821 gaccaatctc atagagggca tcatcgccat actctgaatc gggatatttc aacaacaatt
    20881 gctcgagacg ataaattttg ttcaaataat ttttttgtaa tccttctacg tatgctgact
    20941 gaaaaataga ataatccgct gtattgggag atgtaaaagc agctttttca taaaaggaaa
    21001 gtgcattttt aaaatttctg gcataaaaat gacaatctcc tgctcgagtt aacgcatcag
    21061 catagagcat tgatttctct gaattttcat tttcaagata tttggaaaac cattccaatg
    21121 ccttgatata atttttctga tcaaaatatc cataacccaa agaataatag gcatatatta
    21181 aattttcgct gtttctggac aagggatttt taatgaatgt ttccaaatct acaattgctt
    21241 gatcgggatt gttggtgcga tagtagcatt cggctctcca gaataagcat tcggctgaat
    21301 attttccttt gggtgcagat gtatgggcaa gagaaaaata ttcgatagct tttccataat
    21361 ccttctgagc aaaggcattg acgcccatct gatagagaag atattgtttg gtctcggtta
    21421 aagatgtatt taattttttt attttcaaaa tagattgata agccagttca taattttttg
    21481 tagttagata aacggaagtc aaatattgat aagcctcgtc agcatatttg gagtttggaa
    21541 actcggagag aaaacgggta aaagctgcaa ccgattcccc gaatgctgaa gttgtttcat
    21601 aacaagttaa agcataatta aataaagctt cttctcgaac cgttggatta aagtgcgttt
    21661 gcaaagcggc ttcaaaagaa aggcgggcat tggttttatc gttcaagaaa acatatgatg
    21721 ttcccagatg aagataggca ttttcggaaa tttcatcagg ttcggtggta acttttgata
    21781 gatattggat ggcattggaa taatctttca gttgaaaata ggacaatccc aataaataaa
    21841 catcgtttct gagaacttta ggggacagat tttcatactt tttgaaataa gatatggctt
    21901 gtgcataatt ttgttcgcca taagccaatt ctccaagtat tcggaaaatt tctgcattgt
    21961 tattattgtt gggatatttt tgaagtagaa agtcagcttt ttctttcaat tttgcgtatt
    22021 gtttttcaac ataataaatt tgtaccaaaa aataaggaac actaatttga tagtcaggat
    22081 gattttctat agctaaaaaa tcggggaggg ctttttgata atttcccatc atatattccg
    22141 aataggcata gtaatatgtt gcatccacac gaaagggaga attctgaagt tttaatcgag
    22201 aaaagatttt tccggcttct tcgtaatgtt gtgtttgaat tgcggtatag cctttgtaaa
    22261 aaaggaattc gggaatttta ttttcagaca gctcctgttc gtttacttga gaaaaatttt
    22321 ctctggcaac tgtgtagttt ttctgttcgc agttaagtac cccaatcata aaataaattt
    22381 cctcagaaaa aggtgtataa ggatgtttgg tgagaaaagt ctgtaaaagt gaaagagcgt
    22441 tttcttttct aagttcgtat gcattggccg acaggaaata ttcggcttcc tgaaactgtc
    22501 catcagaggg aaatgttttt ttttctaaaa actgttcaaa acttcggtaa gaggcagcgt
    22561 atttttgttg aaaataaagt tctttccctt gaatgaaatc tttatcttcg gaggcaaaaa
    22621 ataaggtgtg ttgtgcggaa agaaataagt tgcttaaaca aagaaaaaga ataagagtta
    22681 aaacaggaat attttttttt gccattttta tagaatttat tttttacaaa caaaagagca
    22741 taattaagca ggacaaaatg tggaaattta ttgcaaaatt aaatgttttg acactttttg
    22801 ccaatttgtt cccgtagaaa aatttcaact gcttttttaa ccgattttaa accaaaaagt
    22861 tcaagttgaa ttttttcaag aggcacataa aagcagtctt ccacatcatc cgaaggtatg
    22921 ggtatcaggt ctttttttat ttgacagaga aaaaacaaat cgagcgtagg aataagcaaa
    22981 ttgctgtatt cgtattcgtt aggtaaggag aaaaggtaag ttggattttc aacttcaagc
    23041 tgtaattctt ccatgatttc ccttttcaat gcttcttctg ctgtttcata attgtctaca
    23101 aagcctccgg gtaaatccag tgttcctttg gcgggatctt tttttcgacg acaaacgagc
    23161 aactcttttt tttcgtttaa aataaaagca gccactgctg ctgatgcatt caggtaataa
    23221 acaaaaccac aagccttaca acgttttgat ttttcgttgt ttatctcaaa atctgcagaa
    23281 ccacaggaag gacaaaaaga aaatttcgaa aaaaaatcca ttacttgttc tttcccttga
    23341 tatgtttttt tcatttaaag aatatcttta aaattaaaga ttacagttga aaattgtagg
    23401 catgtcgtac aataaacatg ccatttgata attnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23521 nnnnnnnnnn nnntttgtaa ttgaaaaagt tgtttataac tttgtcttaa ctcgtttatt
    23581 tattgaattt aaaatgttat ttttgcaaaa aaattaatac gtatatgggc aaaaagttat
    23641 tgcttcccgg agatgatgag ttcaaagacc ttgttcacag gtatgagcag tacctttcgg
    23701 gcaatgcaaa tggatacttc gatgtggaag aatttgaaaa tattgcggaa tactatttgc
    23761 tgagaggaag aaccaaagat tcttccaggg ctattgattt tggcatgaaa ttacacccgg
    23821 gcagtacagc actcaaagct aaacgggcaa aaatatattt ggctgttgat gaagtaaaaa
    23881 aagcttatca attgctttca tcttttaccg aaagcgatta cgaaacctta ttgttgaaaa
    23941 ttgaagccct tgttcgcatg ggtcgtcatg ctgaagctga taaattgata aaaggcttga
    24001 caacagaaaa aacagccgat ttggataata tctttctcga tatagcttat atttatctcg
    24061 agaacaataa atttgagaaa gcttcggaat ttttattgaa aggctttcaa attaatccaa
    24121 agaatatcga tttacttttt gaattagctt ttagttttga acagatggaa gaaaaagaaa
    24181 aagctattga aatttataac cgtattcttg atttggatgc tttttccgga gaagcttggt
    24241 ttaatatagg acaaatttat ttcaatttgc aggaatttcc taaggcttta gaagcttatg
    24301 attttgtgct ggctattgat cccaatgaca gtctggccca tctgcaaaag gcacatgttc
    24361 attatcagct cgaagagttt tatactgcca ttgaatatta tgcccattat gctgccatga
    24421 ccgatgaccc agcttcgact agcctctata ttgccgaatg ttatgaaaaa cttgagcagt
    24481 acgacaaagc tgtagattat tataaatatt tacttgaaca agatccggat aacaccgacc
    24541 tgcttatttc cattggatct tgtttattgg aacaagaaaa attctcagca agcattgaat
    24601 atctttcacg tgccatagaa cttaatgatt cattggccga tgcttgggtt tatctggctg
    24661 aagcttattt agccatcgat aatctggata ttgcattaaa gtcttatttg cttgcacttc
    24721 aactcgatag tgagcagcct gaagttataa tggctgtagc aaatatttat ttggaaaagg
    24781 gagattacga gactgcatta aatttttatc tggctgttga ggcaatgagc aattcattgg
    24841 aaattgtcaa tgtttttatt gctgccactt attataaatt ggggaataag accgaagctt
    24901 atcgttactt gaaaaaagga atagaggata atgaagatgt gctaaatctg tttttggaaa
    24961 tttgtcccga agctatcgag acgatccatt aatttcctat agatgcagtt attcaaaaat
    25021 ttttttaccg cgtattgtaa ttttttttta tgccggtggc taccttatac ttaagaaata
    25081 aattttgatg aaaatgagac tgtatgggtt gattggttat ccgctaaaac actctttttc
    25141 aaaacgttat tttgcagaaa aatttaggag ggaaggaatt gatgctcgat acgatttata
    25201 tgaacttcaa cgcatagatg atttccctga actggtcaaa atgcctggcc tttgcggctt
    25261 gaatgtaacc attccatata aggaaagcgt tattcctttt ctaaatgaat tggacgaagt
    25321 ggctgaaaag ataagggcag taaatgtcat taagttttta aggaaaaaca atcatttgtg
    25381 gctcaaaggc tataattcgg atattattgg ttttgaaaag gccataatcc cttttttgaa
    25441 aacttttcat gacaaagcat tgctactcgg aaccggagga gcagccaaag cgatttcttt
    25501 tgttcttgca gctcataata ttaaagttac tcatgtttca cgaaagccag gcatcaataa
    25561 aataacctac aatgatttaa acgaagagtt gatgcgtgag cataaattaa ttattaatgc
    25621 cacaccactt ggaacctatc ctaacctttg ctcatgcccc gacatccctt atgaacatct
    25681 tacgccacaa catatattgt ttgatgttgt ttacaaccca cctaaaactt tattcttgaa
    25741 aaaaggggaa gaacaaggag cgaccataat caatggcgaa gccatgttga gaggacaagc
    25801 agaagctgca tgggaaatat ggaacaaata gaagatagta tagtttattt tttcagagca
    25861 agagaggaag gactctttcc ccttttgctc cgatggaaaa aaaatgacta taattgttca
    25921 ccaaacgttt tgataggcaa tcattaaaat tatgctgatt tttttgatta tactttcctt
    25981 atttattggg aaaatattta ttgaaaaaca atatcatatc ctgccatgaa gccttatcgg
    26041 ctgcttcgtt atatgcaatg ggcatattga attttttccc tgcttcagta gaatatttat
    26101 tggtaaaagc atgagtagca ccttcataat ttttgaattc gtaagttata ttgtattttt
    26161 gcatctcact tttaaaatta tctatatctg cttgtgtaac aaacatatct gctgctccat
    26221 tacaaataag cacttttgta tttttcatcg ccggactggc tttaaagcca ttgagcccac
    26281 catgaaaact tacaaccgtt tccaaaggca gtcccattgc agcagcattt agcaccactg
    26341 tgcctccaaa acaatagcca attgcactaa catgtttttt atcaacagtt ttttctgaag
    26401 tcagaatttc ataacctttt tccatccgct ctttaagtaa ttctggatga gaaaaaatat
    26461 atcccgctgc gttttgagct tctgcagcag tatcatatac cttaccgtct ccgtacatat
    26521 caactgccat agccacatag cctaactctg caagcatgtc tgtacgcatt tttatgtatt
    26581 cgttaaatcc ccaccactca tgcacaacca gaatgccggg ttttgttttt tttccatcat
    26641 ctttccatgc aagatatcct ctaaaatttt gatcgccaat tttatacgaa atactttttg
    26701 tttgaatatc tgattttttg tttgagttgt tgttttgagc tgttccaaaa acaggtaata
    26761 acagcatgat gccaaacata attgatataa ttgtcttttt cattgtttta aaattaattt
    26821 attatttata ctttgattga ttgatttgat ttttgaaaat atataaaatc tattaatgag
    26881 aatagaaatt gttgttaaat ttcacatcca tccggtccgc aaactccgcc attttcaccg
    26941 gaaaactcaa taatatcggt ttttttgtgt tcagctaaag ctctgttgag cgcttgtaaa
    27001 aaaaccgatt catcctgtgc tcccgaaacg gcaaatttat cgtcgaacag aaaatatggc
    27061 acacctctga cgtagagctg atgcgcatgg gcaatatcgt gtttgacttc atcgctataa
    27121 gcatccgaaa gtaatacttc gtttgtttcc gtagcttcca aacccacttc ctttgccaat
    27181 tgaagtaaaa tttcattgtc atcgatattt ttcccatcag taaaataagc agcaaaaagt
    27241 ttttcttcca tttcgttttg cttgccgtgt tttttggcca gatgcaataa tcgatgagcc
    27301 ttgaaggaat ttactacaat tgccttttca aaatgaaatt ccaatccgac ttccttagcc
    27361 atctgtgtaa tgaaggcagt ggattggagc gcttgctcca ttgaccaacc tttttcggca
    27421 gccaaatgtt ccattgtttt caactcagga ttggtaacgg caaaaggatc gagttgaaaa
    27481 cttttccatt ctacttgaac attttccctg taagggaatt tttcaagcgc tttttcaaat
    27541 ttatgtttgc caatatagca gaaagggcaa actacatcac tccagatttc aactttaatc
    27601 acaaaattat ttttattttc cattttttat tgttaaatta ttgtcagttt tagcggataa
    27661 gtccgggaac tttttcgcca tgaataaatt ttaatatttt ttgtaccgca tgttcagtcc
    27721 caatcagcaa ggcattgtga accatagccg aattgtgggg tgaacccaac aagttgggta
    27781 attcaaaaaa cggataatga agttcaaatt ttcgatcctt aaatggttct acccaccagg
    27841 catcgatccc cgcataaaaa ttcggatgtg ttttcagatg ttcatacaac gcagcttcct
    27901 caacaatcgc accgcgtgcc acattcacca gcacggcatc ttctttcatc aattccaatc
    27961 tttctttgtt gatcaaacct tctgtctctt cattcagcgc tatggaaagg atgagactat
    28021 cagcattttg cagaacatag tccaaatctt tcaaggttcc aataaaatcg acctcctcat
    28081 ccgttttacc ggaactgttg gtcgccatca acttcacacc aaaaggtcgc aataatctgg
    28141 ctgtagcttt tccgatagct ccaaaaccaa ttattccggc aaccgaacct ttcagaaatt
    28201 tggtttgact ttttatctga ctgaaattgc cctttgccaa gtcgttgtga taaacataaa
    28261 gccttttgct gagcgccaac aacatagcca tcacatgttc ggccattggt tcggcataag
    28321 ccccctgatt ggcagctatc tgaagtttct caggaaaatc ttccaaccga atatgatcat
    28381 agccggcaga aagtaattgc ataaacctaa tattatccat aggcaaattt tgtttgttaa
    28441 tgccttcctg caccggattc cacgacaaaa ggacatcggc tttagtcatc agattttcca
    28501 attcttcttt tgtgcaatct tccttaaagt aaatttgcat ttcctttccc aatacattct
    28561 ttaaatactc tttttgttcc ggaaaagttt taaaagttac tattatagtc attttctttt
    28621 ttatcatttt atttgattga tccacttttc gagtgtttca taaggctgtg caccggttaa
    28681 cttgtatttg ccgttggcaa tcagtgtggg taccgaattg atgccatact gataagcaag
    28741 ataaaagtcc tcatcaagca tttgttttac tttttcgctg tgaaaatctt tctcccattg
    28801 ttcgacattc aatcccacct cacgcgcaca catttttagc acttcaacat cattaatatt
    28861 aagacattca gtcaaatgag ctttctgaat acgattaaat attttgtgat gcatttcatt
    28921 tccaccctgc aattcggcag ccttacaagc cagcaatccc cgagtagaat acggatagtc
    28981 aaaagttcgg gttgccatta attcgggatg catgcgatga gcatcatcat tcatgttggc
    29041 agctctccaa tgccctaaaa tttgtatttt tccttcctcc ttcgaaccga aaattttttc
    29101 tatcccattt ggttcgggag ccagtgcaaa tgatcgatga ataacctcaa tgcgatcaaa
    29161 atatttctct actaattttt ccactctggg cgaaagtgca taacaccatg cgcaaagtac
    29221 atcgtggaaa aattctatgg taattttatt ttgttccata atttttttaa ccaaaacgat
    29281 acgacgaaat tttaacgttc aaaagtgttc ccgaaaaatc ctgtttgcct aagtcgtttc
    29341 cggcagctcc ggcaaccaca aaaagcggaa ccaaatgttc gcttcgcgga tgcgattctc
    29401 ttgcaccggg cgcacttaac cactgagtaa gaagttgatt acgaatttcc ggatttttat
    29461 tttctactgc atgagtcaac catttgtcaa actgttccga aattttcgaa atagcctgat
    29521 tgcccgacat cattccacgc aaattatgaa aactcattcc ggaaccaata ataagtaccc
    29581 cttccgagcg caaagattca agtgcttttc ccatagcaat atgctgagcc ggtcgaagtc
    29641 ccttaatgag cgaaagttgt accacaggaa tatcagcttg aggaaaagct accattaacg
    29701 gtacaaaaac gccgtgatca aatccgcgaa gattttcacg agctgttgta aaaccggctt
    29761 cgagcaacag gttttcaatc tttaacgcca gttgaggatc gccgggagca ggccagctca
    29821 attgataagt aaattcgggg aaaccgtaat aatcgtacag caatgacgga tgctcaccaa
    29881 aattgacggt tggcaccgat tcttcccaat gacctgagac gacaagaatg gctttaattt
    29941 tatcatgaaa atcattgcca atttttttta aataattacg cagcattcca tatccctgag
    30001 ggtcgcccat gaagtcatcc atcacatgcc atggcccacc tccatgagga ataaagaata
    30061 ccggtaattt ttctgaaatt ttcatgttgt tgaatcaatc tgaaaataga cagattgtgt
    30121 ccattctcaa ttttactttt ctctgactgt ttttaaagca gttccccaag ctatgagctg
    30181 atccagcatg gcatgcaagc tgccttcgtg tgaagaagca ggtttaaaaa cactgaaatt
    30241 ttcaaaatcc gtaaagagtg aaagtgttac ctgagctcta acgtcggcta tctgaatttc
    30301 acccatgatt tggcgaagct gttctacagc tcttgcaccg ccggcagagc cataacttac
    30361 aaatcctgct gctttgttgt tccattcggc ataaacaaaa tcgatagcat tttttaaagc
    30421 tgctgtcggt gcatgattat attctggtgt tacaaaaaca tacgcatcca tttcgttgat
    30481 tttctctgcc catttttttg tatgttcctt ttgatactga tgcatggaag ccggaatcat
    30541 ttcatcgagc aacggcaaat tataatccgc aatatcaact atttcaaaaa ttgcatcatt
    30601 acgtttactg gctacctcat aaacccattt tgcaacagct tccgagttac ggcccgggcg
    30661 cgtacttcca actattattc ctatttttat cataatcttt tcatttttta ctgtttattt
    30721 tttcttccaa ttcatccagc ctatcgttgg ccatgtgcac catcatggtt acccgtacaa
    30781 aaggcaaagc caacaagagc aaagccaacg agataatcag ttgaaaccag ttgaaggcaa
    30841 cgatatttac aataattccg ataagtccaa ttaaagcggc aataattcca cccggtattc
    30901 caagtggaca tctgagtttt ttctttgttt ccattttttt gatttttaat tactgataat
    30961 tttttaattt ttctatcaac tcttctttcg tcggcacgct tttgaacacc accttgttgt
    31021 caatcaacaa agtcggaacg gtatggatac ccaatttcat ggttcgtatc attccgcttg
    31081 gtgaattggc gagggtggtt ttcagtgaaa atttatcctc aaactgaggt aaaacttctt
    31141 tcagcatttg cttcgtaagt ttacagttcg gacaagtcaa cgtataaaag agttcgatag
    31201 ttgttttttt attcatttta aaggatttat aaaatttctt tatgcttaaa aagtataaat
    31261 attactcacg acaaaaataa aattgaaaaa aatattcttg agactgtaat ttttttgtaa
    31321 aatttgccgt tccttgtact tttcgtaagt ctgttgtctg caaaatattt ttttataaat
    31381 aaatttattt caaatttgta taaataacat gcattaagga aaaaagttca tgtttttttg
    31441 ataaagcatt tgccatttac aaaataaaat tgctgttttt taaagcaaat tattgtaacg
    31501 tattgtaaca ataaaaaatt taacgacttt cttcagctta aattttgctt ttgctttgtt
    31561 tccatattca aagtttaagg agaaaggtaa aaaatgtatt tttattcgtt agttcaaaaa
    31621 ttcaaaaata tcccgtaatt ttgcaccgca aaaaagaaat taactgcaat tgccttctta
    31681 cctcgttttt tcttcggaag tataaggttt aaaaacagct caatattatt aatggatttg
    31741 gttggatttt ttattcttgg aatagcggca ggaattttgt cgggattgtt tggcattgga
    31801 ggaggaatcg ttatcgttcc ctcgttgata gtgatttttg gtttggacat tttggatgcc
    31861 aatgctgttt cgctggcagc catgttgttg ccggttggaa ttttaggcgt atgggcttat
    31921 tacaaagaag gttatgtaaa tattggcgag tcgctttggg tagcattggg gttgttcttg
    31981 gggtcatttg ttggaggtgg aattgccgta aatattggcg aaagtttgct tgccaagtta
    32041 tatgccggaa ttttaatcta tattgccatc agttattttc aattaccaaa gtattttcgg
    32101 aaaaagcagc cgatagaaaa agaagaaaaa ccacgcgaaa aaaaatcatt ctgggcattt
    32161 gttctgatag gtttggcagc cggagttttt gccggattat ttggcaaagg cggcgggctg
    32221 gttattgttc cgatgctgat tcattttttt cattacgaca gtcgttctgc tgctgcaact
    32281 tcattggctg ccttgcaact tccggtcggc ttgccaagcg tactgattta tgctgaaaac
    32341 ggacatttga atttgctgtt tgccgcactg atcgctttgg gtatttttgt aggagtttta
    32401 ataggttcaa aaatcggcat taaaattcca ccatcaattt tcaaaaaaat ctatgcggtt
    32461 tttctgctgg caattgctgt ttatatggca tttgaatata tttgaaacaa ttgatttcac
    32521 cgaatcgaaa tactttaata aactttatga ttatccgcaa tgattcctat tgtaaattct
    32581 tgacttgaaa tcagtgttgt agctcacagc agcaacaagc aacctgtcga agattgcatc
    32641 accaaaataa
//
LOCUS       DAOSIB010000015        31939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_1596_length_31939_cov_12.062853, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000015 DAOSIB010000000
VERSION     DAOSIB010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 31939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31939
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1596_length_31939_cov_12.062853"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..474
                     /locus_tag="PLD44_02555"
     CDS             184..474
                     /locus_tag="PLD44_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4491 family protein"
                     /protein_id="HOF98252.1"
                     /translation="MINFNGLLVGFAVFLLIGIFHPIVIKAEYFWGVRSWWIFFIIGS
                     IAAVVSLFVEKEIPSILLGVFAFSCFWSIREIFQQKRRVEKGWFPKNPKRKK"
     gene            613..1323
                     /gene="pyrH"
                     /locus_tag="PLD44_02560"
     CDS             613..1323
                     /gene="pyrH"
                     /locus_tag="PLD44_02560"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOF98253.1"
                     /translation="MLYYRRILLKLSGESLMGGKQYGLDENRLQEYAAQIAEVAKMGV
                     QVGIVIGGGNIFRGLSGTKKGFDRILGDQMGMLATVINSLALHSALQAEGATSHVLTA
                     IRMEPVGEYYSKQKAVSSLEKGEIVIISGGTGNPFFTTDTASSLRAIEIGADIMLKGT
                     RVDGVYTADPEKNSDAERFEEITYDEVYRRNLKIMDLTATTLCKENNMPIYVFNMDTI
                     GNLKKVILGEKIGTLIKN"
     gene            1434..1994
                     /gene="frr"
                     /locus_tag="PLD44_02565"
     CDS             1434..1994
                     /gene="frr"
                     /locus_tag="PLD44_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOF98254.1"
                     /translation="MQDVKPILDEAKESMETTIQFLENALAHIRAGKANPRILDAVRV
                     DYYGVHVPLANVASITTPDAKTILIQPWEKAMLKPIEKAILNSDLGINPTDNGEVIRL
                     SMPPLTEERRKQLVKQVRMEVEEARIRIRNARRNAIDHIKKMIKEGLPEDVEKDAEEQ
                     VQKLHDDYIKIIDEMLALKEKEIMTV"
     gene            2142..3080
                     /gene="rsgA"
                     /locus_tag="PLD44_02570"
     CDS             2142..3080
                     /gene="rsgA"
                     /locus_tag="PLD44_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOF98255.1"
                     /translation="MLGTVVKSTGSWYLVETEEKERVECKIKGNFRLKGIRSTNPIAV
                     GDRVEFEKNEDGIGLINRIYDRKNYIIRRSSNLSKEFSIMAANLDIAALIITLNYPET
                     STVFIDRFLAVTEAYNVPACLIINKTDLYGKEDVEYMEALAYLYENIGYPVFKISVLK
                     GENLSAIKEFFTGKTVLLAGNSGVGKSSLLNALSPEARAKTADISFYHNKGMHTTTYS
                     EMYRLPDGGYIIDTPGIKGFGLIDMEESEVDHYFKEIFKISSQCKYDNCLHIQEPECA
                     VREAVEKGEISWSRYQSYLSVLEDCHAEKYRKDEYH"
     gene            3210..9773
                     /locus_tag="PLD44_02575"
     CDS             3210..9773
                     /locus_tag="PLD44_02575"
                     /inference="COORDINATES: protein motif:HMM:NF040284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOF98256.1"
                     /translation="MKKYLRVFLALTEMIFFSSYSFAAFHFTDPNENFLYQKNIFSFY
                     PVNNGNTLSATINADKLGVCINGTSPIITFTATNGTAPYVFTYKINNGALQTITSESG
                     KNTASIHVPTNTAGIFNYVLVSISDATPITQNISNQQVTVTVGNIPSVDFTFSDENCA
                     GKNITFTPINLPAGADYSFMWEFGDNTTSTDSVAHHIFMTAIGNGTQTFTVKLTVTDN
                     LTACTNSKQKTITLSQHPDPKLETSAESDTLNGQVTFMSCSKVATEFVFTNPNENNPY
                     IVNYTIDWGDGSPIFSESTWSLLTHTYNLGMNTLKYTVQGQNGCSVTKEYKIFVGSNP
                     AGGILSPGNTDICVNEQLTFPIAGIQNNPPGTIYMVSFNDGTPEEVFYDNTRTQITHT
                     FTNGSCGTYSYDGRNYYPNSFMVSMTAKNPCSPSGTSSAVTPIYVSIPPEANFNANNS
                     TCVNTDFFIQNTSRRGYVASSSGCTSGDKFVWSISPTEGVTVVSGSLGNTRGLNDEQY
                     SSWISGSDNVILKFANPGNYTIKIKIANRCGASDTTQTICVEPVVTPSFTLDKNKGCA
                     PFTVKVNNTTDTTKLCSNPSYNWTVGYQPANCGTTSSFDYVDHTSTSSKNPHITFNNP
                     GIYTLTMNVYTNCSVQTVSQQVTVTRPPLVSIGNIPDFCGSAEISPIATIQTCIPATD
                     SVSYEWSFAGGTPASSTAASPGKITYSSPGNYTVSLTVKNGCGSTVATKTFKINVVPQ
                     ITNTELSQTICSGDSSQSVPLTSTVPGTTFSWTAQASTGITGFISSGTSNTIPAQNII
                     TSANVSGNVTYSIVPKVGTCTGDTVRYVINVYPRPYFTKQPVSSILCKDGAATLLSVS
                     FSKGEGTFSFQWYSNNVKSTSGGTPIPGAVDSVYTPSTSTVGTTYYYCIIEFSSGYCR
                     EIISDIATVTVNEPPQITQQPLASQKICVGGSLPQPLKVKYSGGAGPSFQWYSNNVDS
                     NVGGTAISGANDSIFTSSSFTATGTFYFYVTITFSGNNCDPVTSETAKVEVIPDPSIA
                     QQPLASQTICQNSIAEELSVKVTGGIGKYAYQWYQNTTNSNSGGSIIPNANDSIYMPS
                     TTNTGTLYYYCEITQPNGPGCNAVSNTSQVIVKEGPAITEQPSSLVVCKNEISPKLVV
                     SWKNGVGIPSYQWFSNTLNSNTGGTLIPTATDSVYMPSTAVVGTIYYYCVISFSSGGC
                     SELVSNPAAVTVNQFPVISDINVSICSGQSFNIIPENSGSNIVPTGTTYTWSMPAISP
                     SNSITGASAQSNPQPQVSQTLTNITTSIAIVTYTVKPSANGCDGAEFKISVTVYPAAH
                     IEANVHNITCNGANDGMISLTITGGVPFATGDPYQITWNGPNGFTSHNANISNLSAGD
                     YQLQVLDAGGCPYQATYTIIEPEKIKISGITQKNVSCYGNADGEISFNVSGGTGNYVY
                     SWTKDGTTFTGTNHLTALQAGTYAVSVTDGNACAPQSEEFTIIEPLPLTVSLSDKKNV
                     LCYGDATGSIDVEVTGGTKAEITSGDYDYYYSWTGPENYTSNNKNLTNLKAGDYFLTV
                     RDASNCTVTSDAFTVTQPQELIVSATSTAITCFGDDNASITLNVTGGVEPYSVQWSNY
                     GEGMVQENLSPGTYDITVTDNNGCQKSLQVIIPEPFRFSVKPVVKQISCYGANDGSIK
                     LNFVGGMPPISFAWDDDPNAGVDRNQLKPGVYTVRIHDAQPCDIIRSFTIIEPQPLTV
                     SAQVKDATDCQNVNSGSILLAVTGGRPPYKYEWSNGMTTQNILNVPPGNYSVLVTDSF
                     GCNVTVQHSIQRQLPLYVQVNSDIEFDCLTKNIIQVNQATVTGGVPPYQISWSSGTIS
                     GANNEIMTTGQNGLVFVHVTDALQCEITTSINIQIPEQGISYQLLDCNQRSYQFNALI
                     PIEKADYIYSWDFGDGGVSDIKNPQYNFSKPGNYTVKLKVQNAECSLYYEKTISVEAP
                     PVLSLDKEPIFCIGDSLLLHVSGADYYRWNDGSTADNLLIVKPGTYSVEGTSKAGCSS
                     ILTFMASYFDLFHYTIQTDQEMVTDRQPTIEFWSEDTSNAIYYWDFGDGYKTQGNHVS
                     HSYIISRDGYYEVNLQVINPNGCREYATKRIWIGAPTLPNTITPNGDGKNDLLLKGFH
                     IQVFNRNGLLLFDGREGWDGTYKGKPVSEGTYFYVLMYATPEGLKFASNFVTVIR"
     gene            9784..10692
                     /locus_tag="PLD44_02580"
     CDS             9784..10692
                     /locus_tag="PLD44_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOF98257.1"
                     /translation="MKIRFLIVIFLFAVIVSMFGQSNIRVNNYWENTYYINPASIHDQ
                     YNGEISMAARNQWINFPGSPTTFFGHGTVFLSDFHTQIGMKAMADKIGYTNTYDLAFS
                     YAYQLTLNPTWNLSMGLTVNYQGLNYDLSQVNLTNGDDPSAYEQFKFESNFNSNLGVE
                     LNNKYWKIGLAGQNIFSLFYSFSELFNNTNFLYASYKPYTRNFFSVGGGMCLIQYGNM
                     MQAELNFNGFFKMTPENNAFQLGLFYRTWHEAGALFGIDLTNSFRLSYSYDYNAGAIR
                     RNSYGTHELILTYKIKGEPKCINCWY"
     gene            10892..11395
                     /locus_tag="PLD44_02585"
     CDS             10892..11395
                     /locus_tag="PLD44_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOF98258.1"
                     /translation="MAQHQIDRLDRKILQLISQDARIPFLEVARECNVSGAAIHQRIQ
                     KLRNLGIIKGSEFVIDTYKVGFQTCAYIGIILSDIKMFKSVVEELKKIPEIVECHYAT
                     GKYSMFIKIYAKDNRHLLQIILEKLTVIPGVAHTETFQISLDEIFRRQLFVFDTENEV
                     ENLEEEI"
     gene            11535..11720
                     /locus_tag="PLD44_02590"
     CDS             11535..11720
                     /locus_tag="PLD44_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98259.1"
                     /translation="MKQAKWWTLQQIEENLQEGIFTENFLIEFDLLKKSGVLENNICD
                     CACKLKETIFKAPHLFR"
     gene            complement(11745..12458)
                     /locus_tag="PLD44_02595"
     CDS             complement(11745..12458)
                     /locus_tag="PLD44_02595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOF98260.1"
                     /translation="MEMNQIYALSCLEGLKKLPDHSIDLIFTDPPYYQYRAQNIKGLK
                     NHKDVVTEFEFDDFKSEEEYLQFLEEVLKECYRVSKPGASGYLWCGDDFVSFLNRMVE
                     RAGFQFRKVIHWHKTNPFPAVHPRKMFANSMELIVHFSKGTPKTWNYKSTNEMHNFIE
                     APICMGKERTIHRTQKPLKVCIPYIEISSNEGDLVLDPFMGSGTTAVACKMLKRNFIG
                     FEINPLYCEIANQRLQKVE"
     gene            complement(12489..13598)
                     /gene="meaB"
                     /locus_tag="PLD44_02600"
     CDS             complement(12489..13598)
                     /gene="meaB"
                     /locus_tag="PLD44_02600"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOF98261.1"
                     /translation="MHFHQKHPENDPSFKNLSVNDGVNKPPIVNPYQKVKQKRKIYSA
                     SEYVKGILSGDISLLSQAVTLVESSLPEHQNIAQEVIEKCLPYSGKSVRLGITGVPGA
                     GKSTFIEALGMYLVQKGHRLAVLAIDPSSERTKGSILGDKTRMEELSVSKNAFIRPSP
                     SAGTLGGVARKTRESIILCEAAGFDRVFVETVGVGQSETAVHSMVDFFLLLQIAGAGD
                     ELQGIKRGIMEMADGIVINKCDGNNIEKAQLAKIQHQNALQLFPPAESGWKPEVLTCS
                     AIEKTGIEDVWNMVERYVEYTKANGFFEYKRNDQAKYWMYETINEQLKAHFYQNEEIR
                     KLLPEIENKILTNELTSFVAAKKLLETYFGKINKK"
     gene            complement(13679..14767)
                     /locus_tag="PLD44_02605"
     CDS             complement(13679..14767)
                     /locus_tag="PLD44_02605"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOF98262.1"
                     /translation="MNRYIKKTVSIIFMMLLFVSASNAQKAVIQFQEKIHDFGQIKEE
                     DGKVSYQFKFTNTGTGPLVISRVQASCGCTTPNWTKEPIEPGKSGSIMVTYNPLGRPG
                     IFNKTITVFSNATEEQVQLQIKGEVIPKTDENSDYPISVSGIRLKTKIVQMNNVNKGT
                     IQSRSISFKNTTSQNVRITFENLPSYVTSEVIPDITQPNEEGKITFYLNSKNCQQWGP
                     ISDDFYLIVNGQKKFSEDNKLTLYANIVEDFSKLSVSERQNAPILDIPSTTLSLGTFK
                     AGSKVSGKFLIKNIGKDELKIHRIVNNNKELTVSSVQTIKGGSKGELKVTVNTANLAS
                     GNYKKTITLQTNDPNNSFVILVVNWTIS"
     gene            complement(14969..15508)
                     /locus_tag="PLD44_02610"
     CDS             complement(14969..15508)
                     /locus_tag="PLD44_02610"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF98263.1"
                     /translation="MIDFKRMQSSSDAFFQQFYRLYSSAFPENERRSLQNLEKELNNP
                     NFHAYVLMNKNSFVGIFNYWNFEKFIYIEHFAIEEKLRSQYLGTEALKSFLEKVNLPV
                     ILEVEMPNTDAAKRRIEFYHRHEFVELQNYYLQPPYESGGFPIPMQLMSNDYEFAKNN
                     FETIKQTIYLEVYHFKPEV"
     gene            complement(15548..16366)
                     /gene="nagB"
                     /locus_tag="PLD44_02615"
     CDS             complement(15548..16366)
                     /gene="nagB"
                     /locus_tag="PLD44_02615"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOF98264.1"
                     /translation="MRLIIQPDYPTLSQWAANYVAAKILHAQPSAQKPFVLGLPTGSS
                     PLGMYHYLIELNKKGIISFENVITFNMDEYVGLPREHEESYYSFMWKNFFSHIDIKPE
                     NVNILNGNAEDLIAECAAYEEKMKSVGGIELFVGGIGPDGHIAFNEPGSSLSSRTRQK
                     TLTTDTILANSRFFNNDINQVPKTALTVGVGTIMDAREVLILVNGHNKARALRHAVEE
                     PISQMWTVSALQLHPKSIIVCDYDACIELKVGTYKYFLDIEKDYLNPESFNIFP"
     gene            16553..18781
                     /locus_tag="PLD44_02620"
     CDS             16553..18781
                     /locus_tag="PLD44_02620"
                     /inference="COORDINATES: protein motif:HMM:NF016945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase"
                     /protein_id="HOF98265.1"
                     /translation="MKNHLLSILLLVACHQSIYASSYQSFVKNSEALIQRVLPEKANH
                     FVIEQISSEHGKDVFEIAISENGKIVLRGNNELSVAMAFNRYLRDVAHVSYDWFSVSP
                     LQIKGELPLPISTIRMACSAQERFFNNTCTFGYTFPFWNWERWEKFIDWLAMNGVNRP
                     LMLAGQEKVWLNVWQSFGLKKEDIQAYFSGPAHLPWHRMANLDKWGGPLPVSYIEGQY
                     RLQKKILARCRELSMKPVLPAFAGHVPEQLKQIYPEANIHQISPGWGGMDADYTTYFL
                     DPSEKLFSEIQYRFLKEQEKLYGISHRYSADPFNEIDPPSWAPEYIASVAKTIYRSMK
                     NADPEAIWYQMSWTFYYDSEHWTQERLKAMVESVPKGKLIFLDYVAEEEEFYKRTENF
                     FGAPFIWCYLGNFGGNTHLVAPMKKVSKRIVEALKVANCLGVGATLEGLNVNPEIYEM
                     VLEIPCSNQISSFNLDEWIKNYAVRRAGQEDSAVIEAWKLLKEKVLVDEAQVIGNHSV
                     VFQVLPVMDLKESSWTTNPFVPYDNPDLAKALQIMLQANEESRKNDAYCFDVVNLARQ
                     ALGNYGSCLYEKMMTAYREQNLSDFQKYSHQFISLGMEIDLLLGTRKEFLLGSWLSDA
                     RRWGKTREEKIYYERNAREIITTWHKSGGELVDYANRQWNGLMRSYYLPRWEKFIELL
                     QKSLINGTSFEQEDFSAWCIDFERKWVNGEIQENFLEKPSGNAIETAQRLFEKYSTEM
                     LK"
     gene            complement(19251..20462)
                     /locus_tag="PLD44_02625"
     CDS             complement(19251..20462)
                     /locus_tag="PLD44_02625"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOF98266.1"
                     /translation="MKILVLNCGSSSVKYRLLDLENNKVLGSGGVEKIGMKGSLLKHI
                     RQDNQIIILEGEVLEHQTAIEYILGVLTSAKHGCIKSLEEIDAVGHRVVHGAEKFNSS
                     VLITDEVIKKIEECIDIAPLHNPPNLAGINAIGELLPTVPQVAVFDTAFHQTMPEYAY
                     MYAIPYSLYTKYGIRRYGFHGTSHRYVSHRACEFLNMDYVQTKIITVHLGNGASITAI
                     ENGKSVDTSMGFTPLEGLMMGTRSGDVDLGIATFLMEKEMIGPSALSTLFNKHSGVLG
                     VSGISSDMRDIETAIANGNERAKLALDMYEYRIKKYIGAYAAALNGVDLIVFTGGIGE
                     NQAKTRQNIIKGLTYLGIDVDEKANAEARGKEALISSAESRVKVAVIPTDEEFMIASD
                     TMKIINEMKRG"
     gene            complement(20475..21431)
                     /locus_tag="PLD44_02630"
     CDS             complement(20475..21431)
                     /locus_tag="PLD44_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOF98267.1"
                     /translation="MSQPIIEPIEKYGLSKRSRRRTLFSKIGVVGCGNEGSAIATIAL
                     LNGIEVVFLEPTEEKIANAYSRIERKLNKRIQNWGLTENEKKIALSRVHGTTKYKDFA
                     GCDFVIEAVRYDDQTGERHVEERKEVFLQLEKVLSPQAIIASNISTVVITELASELQY
                     KERCIGLHFLSNVPGSNIIEIVRGLYTSDETYEKICQFARLINHQYVAVAESAGLVSI
                     RLFLIELNEACAMLMEGISNVEDIDKVLTVGFGHRLGVFRTADQMGIEKIVKLMENLY
                     QEYGNSKYKPSPVLLRLSRAKHFGIKKGKGFYEYDEFGNVIK"
     gene            complement(21458..22456)
                     /gene="pta"
                     /locus_tag="PLD44_02635"
     CDS             complement(21458..22456)
                     /gene="pta"
                     /locus_tag="PLD44_02635"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOF98268.1"
                     /translation="MDLLNKILERAKANIKRIVLPEGDETRTLQAAEIILKEKAAHLI
                     LLGKEDVILKTASNLHLNHIAEAEIIDPETSPRRAEYIDLLYNIRKNKGITYQEAEEL
                     TKNPLYFACLMIKNGDADGELAGARNFTGDVLRPAFQIIKTLPGIQVVSGAILMFTKT
                     PQYGENGLLVFADCAVNPNPTAEELAQIAVCTAKTTRQIGEIEPRIAMLSFSTKGSAS
                     HELVDKVVKATRLACEMDPTLLIDGELQADAALVPSVAHRKAPESAVAGKANVLIFPD
                     LQSGNIGYKLVERFGGAEAVGPILQGMAAPVNDLSRGCSVEDIFHMITITSNLAIK"
     gene            complement(22648..24720)
                     /locus_tag="PLD44_02640"
     CDS             complement(22648..24720)
                     /locus_tag="PLD44_02640"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOF98269.1"
                     /translation="MKFKTILMAIAVLGAGCTEKENHSVIGKQIPNIENGRMTPEVLW
                     SFGRVGGVQISPDRTKILYSVTYYSIPENKGNTELFIMNVDGTNKHQITHTATKEAEA
                     RWMKDGEHIAFLSSESGSNQLWTMKIDGSERKQISNRDGGINGFLFSPDESKVLFIAD
                     VKYGKTAADKYPDLPKATGRIVTDLMYKHWDEWVESIPHPFIADFKDNTLKNEQDIMK
                     GEPYECPMKPFGGIEQLDWSPDGKIIAYTCRKKTGKEYALSTNSDIYFYYLENGKTEN
                     KTEGMQGYDINPKFSPDGKWMAWESMERDGYESDKNRLFVMNVQSGEKLDLSEGFDQN
                     CNAISWSADSRSIYFVSCWHAVTQIYKTDLQTKSITPITHGVHDYGFVAEARNGLIGV
                     KHSMSKPDEIYFIDPVTGKEAELSFENKELLSKLKMGKVEERWITTTDKKQMQTWVIY
                     PPDFDPAKKYPALLFCEGGPQSTVSQFWSYRWNFQLMAANGYIIVAPNRRGVPGFGKA
                     WLEQISGDYGGQNMKDYLSAIDALSKEPFVNKNKLGCVGASYGGFSVYWLAGHHNKRF
                     KAFIAHDGMFNLEQQYLETDELWFVNWDLGGPYWDRKNPVVRRTYANSPHLFVDKWDT
                     PILIIHGEKDYRILASQAMAAFNAAVLQGVPAEMLIFPDENHWVLQPQNGILWQRTFF
                     EWLDKWLK"
     gene            24984..25334
                     /gene="rplS"
                     /locus_tag="PLD44_02645"
     CDS             24984..25334
                     /gene="rplS"
                     /locus_tag="PLD44_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOF98270.1"
                     /translation="MDLLKIAEQSFEQEVKTYPEFRSGDTITVAYRIKEGNKERIQEF
                     KGDVIKISGHGGKKHFTVRKMSGNVGVERIFPMDSPFIESITVNKHGKVRRAKLYYLR
                     NLTGKKAKIKERRV"
     gene            complement(25420..25746)
                     /locus_tag="PLD44_02650"
     CDS             complement(25420..25746)
                     /locus_tag="PLD44_02650"
                     /inference="COORDINATES: protein motif:HMM:NF027794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5056 domain-containing protein"
                     /protein_id="HOF98271.1"
                     /translation="MTKNEQTEEKLKEFFKTHKTEIPDNGFSLKVMRRLPTEKDHSWI
                     VWLIGATGISFLLYHYIHTGILLNFIENLLVMHFDMLLISIAVFPIVCGIVMLLWGKK
                     EKTFFG"
     gene            complement(25733..26278)
                     /locus_tag="PLD44_02655"
                     /pseudo
     CDS             complement(25733..26278)
                     /locus_tag="PLD44_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668052.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(26262..26888)
                     /locus_tag="PLD44_02660"
     CDS             complement(26262..26888)
                     /locus_tag="PLD44_02660"
                     /inference="COORDINATES: protein motif:HMM:NF039701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HOF98272.1"
                     /translation="MKKFVLLAFSLILFSGIGFAQNKSDESSHPSLPAALENLTQREK
                     DSILLNKLSPEQLLELKKAELENERKKIEGNAREDMPLTGFQIFMIVVAPFLFVIILI
                     WIIYAAKNKESKRKFELYMKALEMGQPVPEDFFQKDKKDKPNSNLKKGVLWTAVGLAL
                     LIYFIINHNNDGLIVGIVPTFVGIGYLVVHFYDRSKEKQPIEQNEQQG"
     gene            27114..29399
                     /locus_tag="PLD44_02665"
     CDS             27114..29399
                     /locus_tag="PLD44_02665"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOF98273.1"
                     /translation="MKTEKFDVVGMSCAACAAHIEKEVGKLEGVKKVNVNLLTNSMVV
                     DFEESNVDTDKIEEAVVEAGYEAHVKSSAEQAASRETKKIDFVQKEQQEMQKRWWISF
                     GFWLPLLYLSMGSMLGWYIPAFFRGEENAMVFAFTQFLLTLPIAMSNEKYFRNGFKML
                     FKAAPNMDSLIAIGSSAAILYGIYGLYKIAYGFGHAQPELVHQFAHDMYFESGATILT
                     LITLGKYLESRSKSQTSRAITQLMDLSPKTATVIRDGTEVEVPAEEVRVGDIVVVRPG
                     QRIPVDGVVESGSSTVDESALTGESIPVLKQSGDRVLSASINKSGYITLKATQVGNDT
                     TLSHIISLVEEASSSKAPISKLADRISRIFVPVVIFIALTAGIVWFFLGYSFDFSLSV
                     AISVLVISCPCALGLATPVAIMVGTGKGAEHGILIKSAVSLEMAHKIDTVVLDKTGTL
                     TMGKPQLTDIIPSPNITENELLQIAASLEKLSEHPLAEAILEETDKRKLETFSVKNFT
                     SLFGKGIVAEAGENFFLIGNRQLMEEQKIDVQNFDNIATQLAEEGKTPLFVASREKVI
                     GVIAAADPLKPTSKEAVNQFRSLGLEVVMLTGDHKKTAAAVQRKLGISTVFSEVLPQD
                     KDKVIVNLQKGGKKVAMVGDGINDAPALMRADLGIAIGAGTDIAIESADVVLMRNDLM
                     DAVSAIRLSNAVIRNIKENLFWAFFYNIIGIPLAAGIFYPLLGWKLSPIFAAAAMSFS
                     SVTVVLNALRLRFFKPSEVKS"
     gene            29679..30137
                     /locus_tag="PLD44_02670"
     CDS             29679..30137
                     /locus_tag="PLD44_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008765078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98274.1"
                     /translation="MENQINGPKKQYFEPMHTCEHILNQTMIRMFGCGRSQNAHIERK
                     KSKCDYLLPQAPTEEQIRELSDRVNAVIDSHLPVTIEFLKREEASGVVDLSKLPDDAS
                     DVLRIVRVGDYDACACIGQHVNNTSEIGHFEIISHDYEEGRWRVRFRLID"
     gene            30152..30874
                     /gene="mtgA"
                     /locus_tag="PLD44_02675"
     CDS             30152..30874
                     /gene="mtgA"
                     /locus_tag="PLD44_02675"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOF98275.1"
                     /translation="MFRKAFKFIKNCVVLFFLISIGWVIVDRFVPVVVTPLMLIRTAE
                     SVFSGETPKISKKWVPIDKISHNMIQAAVAAEDDQFLHHHGFSFEAINQAIEHNKRSR
                     RIRGGSTISQQTAKNVFLWPQRSYVRKGLEAYFTVLIELFWSKERIMEVYLNVIEMGD
                     GIYGVEAASMEYFGKHASKLTKSEAALIAACLPNPRRFNPAHPSGYVLRRQGRIMAMM
                     NKIEPVHFERPEFKFLKHKKQK"
     gene            30877..31542
                     /gene="lipB"
                     /locus_tag="PLD44_02680"
     CDS             30877..31542
                     /gene="lipB"
                     /locus_tag="PLD44_02680"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOF98276.1"
                     /translation="MTRQIEFLDWGLTEYADAWKRQEDLFQLLLQNKKNADFNTHYVV
                     FCQHFPVYTLGKNGHEENLLLNQIQLQTRGASFFRTNRGGDITFHGPGQVVGYFIFDL
                     EKLQLGLKVFIYKLEEVIIHLLEHYGLKGTRLHGATGVWLDAETPRARKICAIGLRSS
                     HFVTMHGFALNVNTDLNYFNYIHPCGFVDKGVTSLQKELFQTVEEEEVKNLLKKQIEK
                     EFL"
BASE COUNT         9604 a         6053 c         6307 g         9975 t
ORIGIN      
        1 aaatcgggat gcccttttta attctatgta agtgttttaa acacatatca gatttgttga
       61 agttgatcca acaaaatttt caaaattact gaatgcgaaa actatttgcc gaattctcga
      121 caaatagttt tttaatttta taaaattatt ttcgatcttt gtatcaaaaa aacaactcat
      181 cttatgataa atttcaatgg tttgttggtt ggctttgcag tttttcttct gataggaatc
      241 tttcacccaa ttgttataaa agccgagtat ttttggggtg tccgaagctg gtggatattt
      301 tttataattg gttcaattgc cgccgttgtt tctttatttg tggaaaaaga aataccctca
      361 attttattgg gagtttttgc attttcttgt ttctggagca tacgcgaaat ttttcaacag
      421 aaacggaggg tggaaaaagg ttggttcccc aaaaacccca aaaggaaaaa ataattttaa
      481 attttgctgt ttttgtgttt gtcataagtt tgtaaatact atttatttat ttatttttgt
      541 gtggttatct gtagtttgtt ctgcataaca acgaaaaaac aaaaatttct tgaaccttaa
      601 aaacatatca aaatgttgta ctacaggcgg atattgttaa aactgagtgg agaatcttta
      661 atgggaggaa aacaatacgg acttgacgaa aaccggctgc aggaatatgc ggctcaaatt
      721 gcagaagttg caaaaatggg agttcaagtt gggatagtga ttggtggagg aaatattttt
      781 cggggtttga gtggaacaaa aaaaggcttt gaccgcattt taggcgacca aatgggaatg
      841 cttgccaccg ttatcaacag tttggctctt cattcggcct tgcaggcaga aggagcgact
      901 tcacacgtgc tcaccgccat acgcatggaa ccggtaggag aatattacag caaacaaaaa
      961 gccgtttcat ctttagaaaa aggtgaaatc gtcattattt ccgggggaac gggaaatccg
     1021 ttttttacca ccgataccgc ttcatcactt cgtgccatag aaatcggtgc cgatatcatg
     1081 ctgaaaggaa cccgagtgga tggcgtttat actgccgatc ccgaaaaaaa cagcgatgca
     1141 gagagatttg aagaaataac ttatgatgaa gtttaccgga gaaatctgaa aataatggat
     1201 ttaaccgcca caaccctatg caaggaaaac aatatgccta tttatgtatt caacatggat
     1261 acaattggaa atcttaaaaa agttattttg ggagaaaaaa ttggcacttt aataaaaaat
     1321 tgataccata aagaaaaatc aatttaaatt gaattgaatt gaattggttt gaaaatcctt
     1381 atcgaaaata aataatcacg gaagaagaat cttaaaaact aaattttatg gacatacaag
     1441 atgtaaaacc aattttggat gaagcaaaag aaagtatgga aacaaccata cagtttttag
     1501 aaaatgcatt ggcacatatt cgtgcaggaa aggctaatcc tcgaattctg gatgcagttc
     1561 gtgtggatta ttatggcgtt catgttcctc ttgccaatgt tgcttccatt acaactcccg
     1621 atgcaaaaac aatccttatt caaccttggg aaaaggcaat gctcaaaccc attgaaaaag
     1681 ctattttaaa ttctgatttg ggaattaatc ccaccgataa tggtgaagtc attcggcttt
     1741 ccatgccacc actgactgaa gaaagacgca aacagctggt taaacaggtt agaatggaag
     1801 tcgaagaagc ccgtattcgg ataagaaatg ctcgcagaaa tgccattgac catatcaaaa
     1861 aaatgattaa ggaagggctt ccagaagacg ttgaaaaaga tgcagaagaa caagttcaaa
     1921 aacttcatga tgattacatt aaaatcattg atgaaatgct tgctctcaag gaaaaggaga
     1981 ttatgaccgt ttaacaaggg tattttcaat ggtaagaatt gataaaactt gctttcgata
     2041 taacctcgga tcaagtttta tcaatctttt tatgaaattt caatgccgta attccaaatc
     2101 tcaaaaaaag gttgttaaaa taaatttgaa gtgaacatgt aatgttgggc accgtcgtaa
     2161 aaagcaccgg cagttggtat ctggttgaaa ctgaagaaaa agaacgggta gaatgtaaaa
     2221 ttaaagggaa ttttcgttta aaagggattc gaagtaccaa tcctattgct gtgggcgacc
     2281 gtgtggaatt tgaaaaaaac gaagatggaa taggcctgat taacagaata tacgacagaa
     2341 aaaattatat tattcgtcgc tcgtccaatt tatccaaaga attcagcatc atggccgcaa
     2401 atctcgatat agcggctctt atcattacgt taaattatcc cgaaacttct accgttttta
     2461 ttgaccgttt tttggctgta accgaagcgt ataatgttcc tgcttgtctg ataatcaaca
     2521 aaactgatct ctatggtaag gaagatgttg agtatatgga agcacttgct tatctttatg
     2581 aaaacattgg gtatcccgtt tttaaaatat cggttttaaa aggtgaaaat ctttcggcaa
     2641 taaaagaatt ttttaccggt aaaacagtac ttttggcagg caattcggga gttggaaaat
     2701 cttcattgct caatgcccta tcgcctgaag ctcgtgccaa aacagccgat atttcttttt
     2761 atcataacaa ggggatgcat actaccactt attcggaaat gtatcgatta cctgatggag
     2821 gttatattat tgacacaccg ggtatcaagg gatttggctt gatcgacatg gaagaatcgg
     2881 aagtagatca ttattttaag gagattttta aaatttcttc acaatgcaag tacgataact
     2941 gtctgcatat acaggaaccg gaatgcgctg taagagaagc tgttgaaaag ggggaaatca
     3001 gctggtcgcg ctatcaaagc tatttaagtg ttttggaaga ttgccacgcc gaaaaataca
     3061 ggaaagatga atatcattaa tttatgcaaa ttattctcaa taaaactatt ttttaaaata
     3121 acttaattat ctttacaagg ttttaatcaa taaaaaatat atgacaacca aataaattta
     3181 atactctgaa aatcaatttt ttataattaa tgaaaaaata tcttcgtgtt tttctggcat
     3241 tgacagaaat gatatttttt tcttcttatt catttgctgc ctttcatttt acagatccga
     3301 acgaaaattt tctatatcaa aaaaatatat tttcatttta ccctgtcaat aatggaaata
     3361 cactttctgc aaccatcaat gcagacaaat tgggcgtttg catcaatgga acatctccga
     3421 taattacttt cacagcaaca aatggaacgg ctccttatgt cttcacttat aaaataaata
     3481 atggagcttt acaaaccata acgtcagaaa gtgggaaaaa cactgcttcc attcatgttc
     3541 cgacaaatac tgccggaatt tttaactatg ttctggtaag tatttcagat gctacgccaa
     3601 ttactcagaa tatttcaaat cagcaagtta ctgttacagt aggcaatatt ccttctgtcg
     3661 attttacttt ttccgacgaa aattgtgcag gaaaaaacat cacatttact cctatcaact
     3721 tacctgccgg cgctgattat tcttttatgt gggaatttgg agacaatacc acttctaccg
     3781 acagtgtagc acatcatata tttatgacgg caataggaaa cggcactcaa acttttacgg
     3841 taaaactgac cgtcactgat aatttgactg cctgtaccaa ttcaaaacaa aaaaccatca
     3901 ccttatctca acatcccgat ccaaaattgg aaacatccgc agaaagtgat acgttgaatg
     3961 gtcaagttac tttcatgtct tgtagcaagg tcgccaccga atttgtattt accaatccca
     4021 atgaaaataa tccctatata gtaaattata ctattgattg gggtgatggt tctccaattt
     4081 tttcggaatc cacatggtca ttactgactc atacttataa tttgggaatg aatacattaa
     4141 aatatacagt tcaagggcaa aatggttgct ccgtaaccaa agaatacaaa atatttgtag
     4201 gctccaatcc tgcaggtggc attcttagtc cgggtaatac ggatatttgt gtaaatgaac
     4261 aattgacttt tccaattgcc ggtattcaaa ataatcctcc cggtaccatt tatatggttt
     4321 catttaacga tggtacacct gaagaggttt tttatgataa tacccgaacc caaataaccc
     4381 atacttttac taatggttct tgtggtactt acagttatga tgggagaaat tattatccca
     4441 attcgtttat ggtgagtatg actgccaaaa atccttgcag cccttcagga acatcatctg
     4501 ctgttactcc aatctacgtt tctattcctc cggaagcaaa tttcaatgcc aacaattcta
     4561 cttgtgtaaa taccgatttt ttcatacaaa acacctcaag aagaggatat gtggcatcaa
     4621 gttcaggatg tacttcagga gataaatttg tatggtctat atctccaacc gaaggtgtta
     4681 ctgtggtaag tggctcatta ggaaatacac gagggttgaa cgatgaacaa tacagctcct
     4741 ggatttctgg gagtgataat gtcattctaa aatttgcaaa tccgggaaac tataccatca
     4801 aaattaaaat tgccaatcgt tgcggagcaa gcgatactac gcaaaccatt tgtgtagaac
     4861 cggtagttac tccatctttt acattggata aaaataaggg ttgtgctcca tttaccgtaa
     4921 aagttaacaa cacaaccgat acgacaaaac tatgcagtaa cccttcatac aattggactg
     4981 tcggttacca gcctgccaat tgcggcacta catcctcatt tgactatgtt gatcacactt
     5041 ctacctcatc aaaaaatcca catattactt ttaacaatcc cggaatatat acattaacaa
     5101 tgaacgttta cacaaactgt tctgttcaaa cggtcagcca gcaagttacg gtaacacgtc
     5161 cacctcttgt ttcgatcggc aatattcccg atttttgcgg tagcgccgaa ataagcccta
     5221 tcgcaactat tcaaacctgt attcccgcta ccgattcagt cagttatgaa tggagtttcg
     5281 cgggaggaac acctgcaagt tccacagccg caagtccggg aaaaattacc tattcatctc
     5341 cgggaaacta caccgtttca ttgacagtaa aaaatggttg tggaagtaca gttgcaacaa
     5401 agacattcaa aataaatgtt gttcctcaaa ttacaaatac cgaattaagc caaaccattt
     5461 gttcaggcga cagttctcaa tccgttccat tgacttccac tgttccggga acaacatttt
     5521 cgtggacagc acaagcctca accggtatta ctggctttat ttcttccgga acaagcaata
     5581 caattccggc tcaaaatata ataaccagtg caaatgtttc cggcaatgta acttattcca
     5641 ttgtaccaaa agtaggtact tgcacaggag atactgtcag atatgtcatc aacgtttatc
     5701 cacgtcccta ttttacgaaa caacccgttt ccagtattct ttgtaaagat ggagcagcaa
     5761 cacttttatc ggtttctttt tcaaaaggag aaggaacttt ttctttccag tggtattcca
     5821 ataatgtaaa atccacttca ggtgggacgc ctattccagg agctgtggac tctgtttata
     5881 caccatcaac ttctacggtt gggacaactt attattattg tatcatagaa ttttcgtcag
     5941 gttattgcag agaaataatt tccgatattg ctacagtaac tgtgaatgaa cctccccaaa
     6001 ttactcaaca accccttgca tcacaaaaaa tatgtgttgg aggtagtttg cctcagccac
     6061 ttaaagtaaa atacagtggt ggtgctggtc catcttttca gtggtatagt aataatgtag
     6121 attccaatgt tggaggaaca gctatctcgg gcgcaaacga ttctatattt acatcatctt
     6181 catttactgc caccggcact ttctattttt atgtaacgat tactttttcg ggcaacaatt
     6241 gcgaccccgt tacaagtgaa acggcaaagg ttgaagttat acccgatccg tccattgcgc
     6301 aacaaccttt agcatcgcaa actatttgtc aaaattcaat tgcagaagaa ctcagcgtaa
     6361 aagttaccgg aggaattggg aaatatgcct atcaatggta ccaaaacacc acaaatagta
     6421 acagtggagg gtcaataatt cctaatgcaa atgacagtat ctatatgcca tccacaacaa
     6481 ataccggaac tttatattac tattgtgaaa ttacgcagcc caatgggccg ggatgcaatg
     6541 cagtcagcaa tacttcgcaa gtaattgtga aagaaggtcc cgctattaca gaacaacctt
     6601 catcgttggt ggtttgtaaa aatgaaattt cacccaagtt ggtagtttca tggaaaaatg
     6661 gagtaggaat tccttcctat caatggtttt cgaatacgtt gaactcgaat accggtggaa
     6721 ccttaattcc aacagcaact gattcagttt atatgccttc tactgctgtt gtcggaacaa
     6781 tttattatta ttgtgttatt tctttttcat cgggaggatg ttccgaatta gtttccaatc
     6841 cggctgcagt aacggttaat caattccctg tcatttcaga tattaacgtt tcaatttgca
     6901 gtggacaaag tttcaatatt attcctgaaa attccggaag taacatcgtt ccgacaggta
     6961 caacttatac atggtcaatg ccggctattt ctccatcgaa ttcaattact ggtgcaagtg
     7021 cccaatcaaa tcctcaacct caagtaagtc aaactttgac caatataacc acttctatcg
     7081 ctattgtaac ctatacggtc aaaccttcag ccaacggttg tgatggtgca gaattcaaaa
     7141 tttcggttac cgtgtatccg gcagctcata ttgaagcaaa tgttcacaac attacgtgta
     7201 atggagcaaa tgatggaatg ataagtctga caataacagg tggtgtacct tttgctacag
     7261 gcgatccgta tcaaataaca tggaacggac ccaacggatt cacttcacat aatgcaaata
     7321 tttcaaattt atcggcaggt gattatcaac ttcaagtgtt ggatgccgga ggttgtccct
     7381 atcaagctac gtatacaatt atagaacctg aaaaaatcaa gatttccgga ataactcaaa
     7441 aaaatgtgag ttgctatggc aatgccgacg gtgaaatcag cttcaacgta agtggaggaa
     7501 cgggaaatta cgtttactca tggacaaaag atggaacaac gttcacgggc accaatcatc
     7561 tgactgcact tcaagcagga acttatgccg tatcggttac tgacggcaat gcgtgtgctc
     7621 cgcaatcgga agaatttaca attattgaac cgctaccctt aacagtttct ctttccgaca
     7681 aaaagaatgt tctttgctac ggagatgcaa ccggaagtat tgacgtggaa gtaacaggag
     7741 gaaccaaagc cgaaataact tcaggtgatt atgattatta ctattcgtgg acaggacctg
     7801 aaaattatac cagcaacaat aaaaatttaa ccaaccttaa agcgggagat tattttttga
     7861 cagtgcgaga tgcttcaaat tgtactgtaa cttctgacgc ctttacagtt acgcaaccgc
     7921 aagaactgat tgtttcggct acatctaccg ctattacttg ttttggagat gacaacgctt
     7981 ccattacact caacgttacg ggcggtgttg aaccctattc ggttcagtgg agcaactatg
     8041 gcgagggaat ggtgcaggaa aatctttctc ccggaacata cgatataacg gttaccgata
     8101 ataatggatg ccaaaagtca ttacaggtca tcattcccga accattccga ttctccgtaa
     8161 aaccggtggt aaaacaaata agttgttatg gagccaatga tggcagtatc aaactcaatt
     8221 ttgtaggagg aatgccaccc atttcttttg catgggatga tgatccaaat gccggagtag
     8281 ataggaacca attgaaaccg ggcgtttata cggttcgcat tcatgatgcc caaccttgcg
     8341 atattatccg cagttttacc ataatagagc cgcaaccgct gactgtatcg gctcaagtta
     8401 aggatgctac cgattgccaa aacgtaaata gcggttctat tctgcttgca gttacaggag
     8461 gaagaccacc ctacaaatat gaatggtcaa atggaatgac aacacaaaat attttgaatg
     8521 ttcctccggg aaattattca gtattagtaa ctgattcttt tggatgtaat gtcactgttc
     8581 agcattccat tcaaagacaa ctacctttat atgtgcaagt gaatagcgat attgaatttg
     8641 attgcttaac taaaaatatt atccaagtca accaggcaac agtcactgga ggagttccac
     8701 cctaccagat ttcgtggtca agtggaacga ttagtggcgc aaacaatgag atcatgacga
     8761 ccggtcaaaa tggtttggtt tttgttcatg ttactgacgc attgcaatgc gaaatcacaa
     8821 catctattaa cattcaaatt ccggaacaag gtatcagcta tcagcttctc gactgcaatc
     8881 agcgatccta tcagtttaat gcattgattc ccatagagaa agcagactat atctattcat
     8941 gggattttgg cgatggcgga gtttctgata ttaaaaatcc tcaatataat ttttcaaaac
     9001 cgggtaatta tacggtaaaa ttgaaagttc aaaacgcaga atgttcgctg tattatgaaa
     9061 agacaatttc tgttgaagct cctccggtgc tttcactcga taaagaaccc atattctgta
     9121 ttggtgattc tctgttgctt catgtttcag gggcagacta ttatcgctgg aatgatggtt
     9181 ctacagcaga taatttattg attgtcaaac cggggacgta ttctgttgaa ggaacttcaa
     9241 aagccggatg cagttcaatt ttgactttca tggccagtta tttcgattta ttccattata
     9301 ccatacaaac cgatcaggaa atggtaaccg atcgccaacc aacgatagaa ttctggtcgg
     9361 aagacacatc caatgctatt tattactggg attttggtga tggatacaaa actcagggta
     9421 atcacgtttc tcatagttac attatttcaa gagatgggta ttatgaagtt aatttacaag
     9481 tgatcaaccc caatggttgc cgggaatatg ctacaaaacg aatttggatt ggagctccaa
     9541 ctttgcccaa taccattacg cccaatggag atggaaaaaa cgatctgttg ctgaagggat
     9601 ttcatattca ggtattcaac agaaacggac tactgctgtt tgatggcaga gaaggctggg
     9661 atggaacata caaagggaaa ccggtgtctg agggaacata cttttacgtt ttaatgtatg
     9721 ctacacccga aggattgaag tttgcttcaa attttgttac agtaattcgc taaaagcaat
     9781 ttaatgaaaa tacgttttct gatagtgatt ttcttgtttg cagtaattgt ttccatgttt
     9841 ggacagtcaa atattcgcgt aaacaattat tgggaaaaca cttattatat caatcctgct
     9901 tccattcacg atcagtataa tggagaaata agcatggctg ccagaaatca atggataaat
     9961 tttccgggct cgcccactac atttttcgga catggcaccg tttttctttc cgattttcat
    10021 acgcaaattg gaatgaaggc aatggccgat aaaattggtt acaccaatac ctacgatctc
    10081 gctttttcct atgcttacca gttaacatta aatcctacat ggaatctgag tatgggattg
    10141 accgttaatt atcaaggatt gaactacgac ctgagtcagg tcaatttgac gaatggtgat
    10201 gacccttcag catacgaaca atttaaattt gaatccaatt ttaattctaa tctgggtgtg
    10261 gagttaaata acaaatactg gaaaatcggg ttggcagggc aaaatatttt ttcactcttt
    10321 tactctttca gcgaactgtt caataacacc aattttttat atgcctccta caaaccctat
    10381 actcgaaatt ttttcagtgt gggtggagga atgtgtttga ttcagtatgg caacatgatg
    10441 caagcagaat tgaactttaa tggcttcttc aaaatgaccc ctgaaaacaa tgcttttcaa
    10501 cttggtttat tttatcggac atggcacgaa gctggtgccc tttttggcat tgacctgacg
    10561 aattcctttc gcctttccta cagctacgac tataatgccg gtgcaatcag acggaactct
    10621 tacggtacgc acgagttgat tttgacttat aaaatcaaag gagaacctaa atgtatcaat
    10681 tgctggtatt gaaattcgac attttagtag tggactcaat attgctttat aaaataattc
    10741 agactttttc ggattaaaaa atacagaaaa gatgaatatt tttaattttg actatcaatt
    10801 tgtagaaaaa atttagataa ttcaaataaa ttaaatacat ttgtagagaa aaatttttta
    10861 attatttatt tctgagctta aagattaagt tatggcacaa catcaaattg atagattgga
    10921 tcgtaaaatt ttgcaattga tttcacaaga tgcccgtatc ccttttctcg aagttgcacg
    10981 cgaatgtaat gtttcggggg cagcaattca tcagcgcata caaaaacttc gcaatctagg
    11041 aataattaaa ggttctgaat ttgtgataga tacctataag gttggatttc aaacctgcgc
    11101 ttatattggg attattttaa gcgatattaa aatgtttaag tctgtggtag aggagttgaa
    11161 aaaaattcca gaaattgttg aatgccatta tgcaacagga aaatattcca tgtttattaa
    11221 aatttatgca aaagacaacc gacatttgtt acagattata ctcgagaaat taaccgttat
    11281 accaggtgtg gcgcatacag aaacctttca gatttctctg gatgaaattt ttcgtcgtca
    11341 gctttttgtt ttcgatacgg aaaatgaagt agaaaattta gaggaagaga tataataatt
    11401 tctattagct taaattaaaa aattccggtt ttctggattg caacacacaa attacaaaat
    11461 tgttgctgac taccaatata taatataaaa atcctgaaaa ttttgaaaat aaaatagacc
    11521 tccaatcgga aaaagtgaaa caggcaaaat ggtggacgct acaacaaatt gaagaaaatt
    11581 tgcaggaagg aatttttacc gaaaactttc tgatagaatt tgatttgttg aaaaagagtg
    11641 gagttttgga gaataatatt tgcgattgcg catgcaaatt gaaagaaacc attttcaaag
    11701 ctcctcatct gtttcggtaa ggcaaaacct aaaaaatttc ttggttattc aactttttgc
    11761 aatctttggt tggctatttc gcaatagagt ggattgattt caaagccaat aaaatttctt
    11821 ttcagcattt tgcaggccac tgccgtcgtt cccgaaccca tgaacggatc cagtaccaaa
    11881 tcgccttcgt tactgctaat ttcaatgtac ggaatgcaca ctttcaatgg tttttgtgtt
    11941 cgatgaatgg ttctctcttt ccccatgcag ataggtgctt caataaaatt gtgcatttcg
    12001 ttggtgctct tgtaattcca tgttttggga gtgccttttg aaaagtgtac aatcagctcc
    12061 atgctgttgg caaacatttt tcggggatgt acggcaggga aaggattggt tttgtgccag
    12121 tgaatgactt ttctgaattg aaagcctgcc cgttcaacca tacgattgag aaaagacaca
    12181 aaatcgtcgc cgcaccataa atatccgctg gcgccgggtt tgctcacacg ataacattcc
    12241 tttaatactt cttccaaaaa ttgcaaatac tcttcttccg atttaaaatc atcaaattca
    12301 aactcggtaa ccacatcctt atgattttta agtcctttta tgttttgagc ccggtattga
    12361 taatatggtg gatcggtaaa gataagatca atactatggt cgggtaattt ttttaaacct
    12421 tccaaacacg acaaagcata aatttgattc atttccatgt ggggaaaaag ttattgaata
    12481 tttgtctgtt attttttgtt gatttttccg aaatatgttt cgagcaattt ttttgctgcg
    12541 acgaatgatg taagttcatt ggtaagaatc ttgttttcga tttcaggcaa taatttccta
    12601 atttcttcat tttgataaaa atgagccttt agctgttcgt tgatggtttc atacatccag
    12661 tattttgcct gatcgtttcg tttatattcg aaaaaaccgt tggctttggt atattccacg
    12721 tatctttcca ccatattcca cacgtcttcg attcccgtct tttcaatggc cgaacaagtc
    12781 agcacttcgg gtttccatcc cgattcagcg ggaggaaaaa gttgaagggc attttgatgc
    12841 tgtatttttg ccagttgtgc tttttcgata ttatttccgt cgcatttgtt gataacaatt
    12901 ccatcagcca tttccataat gccgcgtttg ataccttgca attcgtctcc ggctccggca
    12961 atttgaagca gcagaaagaa atcgaccata gaatgaacgg cggtttccga ttgtcccacg
    13021 ccgactgttt ccacaaaaac cctgtcgaaa ccggcagctt cgcaaagaat aatgctttcg
    13081 cgcgttttgc gggcaacacc gcccaacgtt cctgcagaag gagaagggcg aataaaagca
    13141 ttttttgaaa cggaaagttc ttccattcgc gttttgtctc ccaaaatgct tcctttggtt
    13201 ctttcgctgc tggggtcgat agccaaaact gccagtctgt gtcctttttg aaccaaatac
    13261 atgcccaatg cttcgataaa ggtacttttg ccggctccgg gaactcccgt tattcccaat
    13321 ctgaccgatt tcccggaata tggcagacat ttttcgatta cttcctgagc aatattttga
    13381 tgttcgggca atgaactttc caccagcgtt acggcttggc tcaacaagga aatatcgccc
    13441 gataatatgc ctttcacata ttctgatgca gaataaattt ttcttttttg tttaaccttc
    13501 tgatagggat tgacaatagg tggcttattt acaccatcat tgacacttaa atttttgaat
    13561 gaaggatcat tttcgggatg tttttgatga aagtgcatag ttgaattttt ggaataaaat
    13621 agccagaatg taaccttttt tacatcctgg ctcgtaattt tctaaaaaga attttttgtc
    13681 agctgattgt ccaatttacg accaggataa caaaactgtt gttcggatcg ttggtttgca
    13741 gcgtaatggt ttttttgtag ttgcccgatg ccaagttggc ggtgttgacg gtaactttca
    13801 gttctccttt gcttccacct tttatggttt gaacggaaga aacagtcaac tctttattgt
    13861 tgttaacaat tcggtgtatt ttcaattcgt cttttccgat attttttatt aagaatttac
    13921 cgctgacttt tgaaccggcc ttaaaggttc ccagtgataa ggtggtggaa ggaatatcca
    13981 gaattggagc gttttgcctt tcgctgacgg aaagtttgct gaaatcttcc actatattgg
    14041 cataaagggt cagtttgttg tcttccgaaa atttcttttg accattgaca atcagataaa
    14101 aatcgtcgga aattgggccc cattgctgac agttttttga gttgagataa aaagtaattt
    14161 tcccttcttc attgggttgt gtaatatcgg gaataacttc tgaagtaaca tacgaaggca
    14221 agttttcaaa agtaattctt acgttttggg aagttgtatt tttaaaggag atgcttctgc
    14281 tttgaatggt tcccttgttg acattgttca tttgaacgat tttggttttc agtctgattc
    14341 cactcacaga tatcggataa tcggaatttt catcagtttt cggtatgact tctcctttaa
    14401 tttgaagttg tacctgttct tctgttgcat tgctgaatac ggtgatagtt ttgttaaaaa
    14461 ttcccggacg tcccaaagga ttataggtaa ccataataga accggattta ccgggttcaa
    14521 taggttcttt tgtccaattc ggtgtggtgc aaccgcaaga tgcctgtact cggctgatta
    14581 cgagcggtcc tgttcctgta ttggtgaact tgaactgata gctcactttt ccgtcttctt
    14641 ccttaatttg accaaagtcg tgaatttttt cctgaaattg aatcactgct ttttgcgcat
    14701 tggaagcgga aacaaataaa agcatcataa aaatgatgga aacggttttt tttatatatc
    14761 tattcataac ttgagaattg tttattctga aaatattttg aaaatttctc tattcagact
    14821 acaaaaatag gtaaaatgcg ctttctttgc acctgaattt tgtctatatc tgattaataa
    14881 tagttaaaaa agaattttcc tttcacctca aatatcttaa ccaggaggag cattataatt
    14941 ctcttagcat tgttggagaa acaaatattt aaacttccgg tttaaagtga taaacttcta
    15001 aataaatggt ttgtttaatt gtttcaaaat tattttttgc aaattcgtaa tcgttgctca
    15061 tcaactgcat ggggatgggg aaacctccgc tttcgtaagg tggctgaagg tagtaattct
    15121 gtaattcgac gaattcatgc cggtgataaa attcaattct tctttttgca gcatcagtat
    15181 tgggcatttc aacttccagt attacaggca aatttacttt ttcaagaaac gatttcaatg
    15241 cctctgtgcc caaatactgg cttctcaact tttcttcaat agcaaaatgt tcgatataaa
    15301 taaatttttc aaaattccag taattgaaaa ttcccacaaa gctgttttta ttcatcaata
    15361 cgtaagcatg aaagtttgga ttgttcagtt ccttttccag attctgtaag cttctgcgtt
    15421 cattttcagg aaaagcagaa gaatacagcc tgtagaattg ctgaaaaaac gcatcagaag
    15481 aggattgcat ccttttaaaa tcaatcatgg caaacaggta ttggtatgtt tgttactacg
    15541 gtttttttca aggaaaaatg ttgaaacttt ccgggttcaa atagtctttt tcaatatcca
    15601 gaaaatattt gtaagtgccc actttcagct caatgcaggc gtcgtaatcg catacgataa
    15661 tacttttggg atgcagttgc agtgcgctca ccgtccacat ctgactgata ggttcttcta
    15721 ctgcatggcg cagagcgcga gccttgttgt gtccattaac gagaatcaaa acttcccgag
    15781 catccattat agttcccaca ccaacggtaa gagcagtttt gggaacctga ttaatatcgt
    15841 tattgaagaa acgcgaattg gcaagtatgg tgtcggtagt caatgttttt tgtcgcgtac
    15901 gcgatgaaag agatgagccc ggctcgttaa atgcgatgtg tccgtccggc cctattcctc
    15961 ctacaaaaag ctcaattcct cccaccgatt tcattttttc ttcgtaagct gcacattctg
    16021 ctatcaaatc ttcagcattg ccattgagaa tatttacatt ttcgggtttg atatcaatat
    16081 ggctgaaaaa gtttttccac atgaaactgt aatagctttc ttcatgttca cggggtaacc
    16141 caacatattc gtccatgttg aaagtgataa cattttcgaa ggaaataatg ccttttttgt
    16201 ttaattcgat taaataatgg tacatgccga gaggcgaaga tcccgttggc aaacccaaaa
    16261 caaaaggttt ttgagcgctt ggctgggcat gaagaatttt tgctgccacg taattggctg
    16321 cccactgcga taaagtcggg taatctggct gaatgatgag tctcatgatg aaaaaggttt
    16381 tttatagttt tatagttttt tacaaaagta cgattatttc tttcgtttta aaagaatatt
    16441 ttaaaaaata ttagtccaac attttttgtt gcacaaacaa attttatctc ttctcatttt
    16501 tgtcgggatg atttgttatt tttgttctga gaaaaccaaa ttttccgtta ttatgaaaaa
    16561 tcacctattg tctattttat tgttggtagc ctgccatcaa agcatttatg catcttctta
    16621 tcaatctttt gttaaaaatt ctgaagcgct cattcaacgc gtgttgcccg aaaaggcgaa
    16681 tcattttgtg attgaacaaa tttcttctga gcacgggaaa gatgtttttg agatcgctat
    16741 atccgaaaat ggaaaaattg tattgcgggg aaacaatgaa ctgtctgttg ctatggcttt
    16801 taatcgttat ttgcgggatg ttgcgcatgt ctcttacgac tggttttccg tatcaccttt
    16861 acaaataaaa ggagaattgc ctttgccgat ttcaacaata agaatggcat gtagtgcgca
    16921 agaaagattt tttaacaata cttgtacttt tggttatact tttccgttct ggaattggga
    16981 acggtgggaa aaatttatcg actggctggc aatgaatggg gtcaaccggc cattaatgct
    17041 ggccggacag gaaaaagtct ggttaaatgt atggcaatct tttggattga aaaaagaaga
    17101 tattcaggct tatttttccg ggcctgcgca tttgccgtgg caccgaatgg cgaatttgga
    17161 taaatggggc ggccccttgc cggtttcgta tatcgaaggt cagtatcggc ttcagaaaaa
    17221 aattctcgcc agatgcagag aattgtctat gaaacctgtt ttgccggctt ttgccggaca
    17281 tgttcccgaa cagctaaaac aaatttatcc ggaggctaac attcatcaaa tttctccggg
    17341 ttggggtgga atggacgccg attatacaac ctattttctc gatccttcag aaaaactatt
    17401 ttccgaaatt caatatcgtt ttctgaaaga acaggaaaaa ctttatggca tttcacatcg
    17461 ctattcggcc gatcctttca acgaaattga cccacccagc tgggctcctg aatatattgc
    17521 ttctgtggca aaaacaattt accgatcgat gaaaaatgct gatccagaag ctatttggta
    17581 tcagatgtcg tggacttttt actacgacag cgaacactgg acgcaggagc ggctcaaagc
    17641 tatggttgaa agtgttccga aagggaaact gatttttttg gattatgtgg cagaggaaga
    17701 agaattttac aagcggacag aaaacttttt tggagcgcca tttatttggt gttatttggg
    17761 aaattttggc gggaataccc atttggttgc gcccatgaaa aaagtttcca aaagaattgt
    17821 agaagcacta aaagtggcaa attgtcttgg cgtgggtgct acgctggaag ggctgaatgt
    17881 gaatcccgaa atttatgaaa tggtattgga aattccttgc agcaatcaaa tttcttcgtt
    17941 caatctggac gaatggataa aaaattatgc tgtcagaaga gccggacagg aagattcggc
    18001 tgtcattgaa gcatggaagt tgttaaaaga aaaagtcttg gtggacgagg ctcaagtaat
    18061 tgggaatcat agcgttgttt ttcaggtttt gccggtcatg gatttaaagg aaagttcatg
    18121 gactaccaat ccatttgttc cttatgacaa tcccgatttg gcgaaagctt tacaaatcat
    18181 gttgcaagca aatgaagaaa gtcgaaaaaa tgatgcctat tgttttgatg tcgttaattt
    18241 ggccaggcaa gcactcggaa attatggcag ttgcctttac gaaaaaatga tgactgctta
    18301 tcgagaacaa aatctctctg attttcaaaa atattctcat cagtttattt cacttggaat
    18361 ggaaattgac ttgttgttgg gaacaagaaa ggaattttta cttggcagtt ggctttcaga
    18421 tgcacgtcgc tggggaaaaa cgagagagga aaaaatctat tacgaaagaa atgcgcgtga
    18481 aattatcacc acatggcata agtccggcgg cgaattggta gattatgcca atcgccaatg
    18541 gaatggcctt atgcggagtt attatttgcc acgttgggaa aaatttattg agctcttgca
    18601 aaagtccttg ataaacggca cttctttcga acaagaagat ttttctgcct ggtgtatcga
    18661 ttttgagaga aaatgggtga atggagaaat tcaagagaat tttttggaaa aacccagtgg
    18721 aaatgctatt gaaacggctc aacgtttgtt tgaaaaatat agtaccgaaa tgctgaaata
    18781 aaaatcgagt agttaggggg attactctac caccctttca cattaccatg cgtgttctta
    18841 cacacagcgg tttcttttaa ttgtttaact tctccagctg aacacattga taaacaagca
    18901 aacatactgt ctggaggatg agtttgggta ctacaaattt gttctaaaaa ataaaactca
    18961 ttgaaatcca gtatgttaca attttaatcg actgaatttg actaccaaac taaccaatat
    19021 cagtgtgttt ttaacaaaac tttaacgtta cgttattcaa atttggaaag ttgagctaaa
    19081 gaaccattga taatagatta aatttaaaat gaatggttgt gtaggacaga tttaatgcct
    19141 aaaaagtaag atagtatttt cttacgaagg aattaagttt tgaagagcga accatatgtc
    19201 ttttattttg aaatacaaaa gaatacggat atcaaattac tgtttgcaaa ttatcctctt
    19261 ttcatttcat tgattatttt catcgtatcg gaagcaatca tgaattcttc atctgtagga
    19321 attacggcaa ctttcaccct cgattctgca gaacttatga gtgcttcttt gccacgtgct
    19381 tcagcattgg ctttttcatc aacatctatc cccagataag tcagtccctt tattatgttt
    19441 tgtctggttt tggcctgatt ttctccaata ccaccggtaa aaacgattaa atctactcca
    19501 ttcaaggcag cagcataagc tccaatatat ttttttatcc ggtactcata catgtcaaga
    19561 gcgagttttg ctctttcatt gccatttgca atggcggttt caatatcgcg catatcggat
    19621 gaaatgcctg aaacgcccaa tacaccgcta tgtttgttga aaagtgtaga aagtgcagaa
    19681 ggcccaatca tttctttttc catcagaaaa gtggcaattc ccaaatccac atctccggag
    19741 cgtgtaccca tcatcaatcc ttccaatggt gtaaatccca tcgaagtatc aaccgattta
    19801 ccgttttcta tggcggtaat ggaggctcca tttcccagat gtacggtgat gattttcgtt
    19861 tgtacgtaat ccatattcaa gaattcacag gcccgatgtg aaacgtagcg gtggctagta
    19921 ccgtgaaacc cgtatcggcg aataccgtat ttggtataaa gagaatatgg aattgcatac
    19981 atgtaggcat attcgggcat tgtttgatga aaggccgtgt cgaaaactgc tacttgtgga
    20041 accgtcggca atagttcgcc gatagcatta ataccggcca aatttggtgg attgtgcaag
    20101 ggagctatat cgatgcattc ttctattttt ttgattactt cgtcagtaat cagcacactt
    20161 gaattgaatt tttcggcacc gtgaactact cgatgcccta cggcatctat ttcttccagc
    20221 gacttaatac aaccatgctt tgcgctggta agcactccca atatatattc aatggctgtc
    20281 tgatgttcca atacttcacc ttccagaata attatctgat tgtcttgtcg gatatgtttc
    20341 aaaagggaac ctttcatgcc tatcttttcc actccacccg aaccaagaac tttgttattc
    20401 tccaaatcga gcaatcggta ttttacggag gaacttccgc aattcaacac taaaattttc
    20461 attttttatc ctccttattt tataacatta ccaaattcat catattcgta aaatcccttt
    20521 ccttttttta tgccaaaatg ttttgcgcga gaaagacgca acaagaccgg cgaaggtttg
    20581 tatttagaat tgccatattc ctgatacaaa ttttccataa gtttgacaat tttttcgatt
    20641 cccatttggt cggctgttcg gaatacaccc aagcgatgac caaaaccaac ggtaagtact
    20701 ttgtctatgt cttcaacgtt ggaaatgcct tccattaaca tggcacaggc ttcgttgagc
    20761 tctattaaaa acaaacgaat actcaccagc cctgccgatt cggcgactgc aacatattga
    20821 tgattgatca gacgggcaaa ttgacaaatt ttttcgtacg tttcgtcgga agtgtaaagc
    20881 cctctgacga tttcaataat atttgatccg ggaacattgg aaaggaaatg aagccctatg
    20941 caacgttcct tatattgcag ttctgatgca agttccgtga taaccacagt ggaaatattg
    21001 gaagcaatga tggcttgagg agacaggact ttttcgagtt gaagaaaaac ttcttttctt
    21061 tcttcaacgt gcctttctcc cgtttggtca tcatagcgta ctgcttcaat gacaaaatcg
    21121 caaccggcaa aatctttata tttcgttgtt ccatgtacgc ggctgagagc aatttttttt
    21181 tcgttctccg tcaatcccca gttctgaatt cttttattca gtttcctttc aatccggctg
    21241 taagcattgg caattttttc ttctgtaggt tccaaaaaaa caacttctat tccgttgagc
    21301 aaagcaatgg ttgcaatagc acttccttcg tttccgcaac cgaccactcc gatttttgaa
    21361 aacaaagtac gtcggcgact cctcttgctc agaccatatt tttctatggg ttcaataatt
    21421 ggttgggaca tattttttta ttgtatggta aaaacactta ttttatcgcc agattggaag
    21481 taatggtaat catgtgaaag atatcctcta ccgagcagcc acgagaaaga tcattgacag
    21541 gtgctgccat tccttgcagt attgggccaa cagcttctgc tccgccaaag cgttccacca
    21601 atttgtatcc aatgtttccc gactgaagat cagggaaaat caatacattt gcttttcctg
    21661 ctactgcact ttcaggcgct tttcgatggg caacagaagg gacgagagct gcatccgcct
    21721 gaagttctcc gtcaattaaa agtgtcgggt ccatttcaca ggcaagacgg gtagctttca
    21781 ctaccttatc taccaactca tggctggcac tgcctttggt ggaaaaactc agcatggcaa
    21841 tgcgtggttc tatttctcca atttggcgag tcgttttagc tgtacagacc gcaatttgag
    21901 ccaattcctc agctgtagga ttgggattta ctgcacagtc ggcaaaaaca agcaatccgt
    21961 tttcgccgta ttgaggcgtt ttggtaaaca tgagaatggc accggaaaca acctgaatgc
    22021 cgggtaaagt tttaattatt tgaaatgcag ggcgaagcac atcccccgta aaattccgag
    22081 ctccggcaag ttccccgtca gcatctccgt ttttaatcat cagacaggca aaataaagcg
    22141 gatttttcgt caattcttca gcttcctgat aggttatgcc tttgtttttg cgaatattgt
    22201 acagcaaatc gatgtattct gctcttcgag gacttgtttc gggatcgata atttctgcct
    22261 cagcaatatg atttaaatgc aaattcgatg ctgtttttag aatgacatct tcttttccca
    22321 gtaaaattaa atgagcagcc ttttctttta atattatttc agctgcctgc aaggtacggg
    22381 tttcatctcc ttcgggcaat acaattcgct taatatttgc ttttgcacgc tccaatattt
    22441 tgttcagtag atccattttt tataattatt tttcactaat ttgctgtaaa caatacctat
    22501 agctcttttg cttttgagca atacaaaagt atccaaaata aattgaaatt gcaatgtaga
    22561 gatggcaatt tttatttttt tatttttttc ttagctgtta catatcgcgt ccaacggatt
    22621 tttcatcaaa aaatgaaaaa tttttgttca ttttaaccat ttatccagcc actcaaagaa
    22681 agtgcgttgc cacaaaattc cgttttgtgg ttgtaaaacc caatgatttt catcagggaa
    22741 aataagcatc tccgcaggaa caccctgcaa aactgctgca ttaaaagctg ccatagcttg
    22801 tgaagcgagg atgcgataat ctttttctcc gtgaataatg agtattggtg tatcccattt
    22861 gtcgacaaat aaatgtggcg aattggcata agttcgacga acgacgggat tttttctgtc
    22921 ccagtagggt ccacccaagt cccaatttac gaaccagagt tcatccgttt caagatattg
    22981 ttgttcgaga ttgaacatac catcatgagc gataaaggcc ttgaatcgtt tgttgtgatg
    23041 ccctgccagc caatatacgg aaaatcctcc atagctggca cccacacatc caagtttgtt
    23101 tttgtttaca aaaggttctt tagacaaggc atcgatggca gaaagataat ctttcatgtt
    23161 ttgaccgcca taatcgccac tgatttgctc gagccaagct ttaccgaagc ctggaacgcc
    23221 tctgcgatta ggagcaacta taatgtagcc attggcagcc ataagctgga aattccagcg
    23281 gtacgaccaa aattggctca cagtggactg aggaccgcct tcgcaaaaaa gtaaagcagg
    23341 atattttttt gcaggatcaa aatccggcgg ataaatgacc caagtttgca tttgcttttt
    23401 gtcggtagtg gtaatccatc gttcttcaac ttttcccatt ttaagttttg acaatagttc
    23461 cttgttctca aaggacaatt ccgcttcctt tcccgtaacc ggatcaataa agtaaatctc
    23521 gtcaggtttg ctcatggaat gtttcactcc gataagacca tttctcgcct ctgctacaaa
    23581 tccatagtcg tgtactccat gtgtgatggg agtaattgat tttgtttgca aatcggtttt
    23641 gtagatttgt gtaacggcgt gccaacaact gacaaaataa atagaacggc tgtcggcact
    23701 ccaagaaatt gcattgcaat tctgatcaaa tccttccgaa agatccaatt tttctccgct
    23761 ttgaacattc attacgaata aacggttttt atccgattcg tatccatcgc gttccatgct
    23821 ttcccaagcc atccattttc cgtcgggaga aaactttgga ttgatgtcgt aaccctgcat
    23881 tccttccgtt ttattctcgg ttttaccatt ttcgagatag taaaagtaaa tatccgaatt
    23941 ggtggaaagg gcatattctt tcccggtttt ttttcggcag gtgtaagcaa taattttccc
    24001 gtcaggactc cagtcgagtt gttcaatacc accaaaaggt ttcatgggac attcataagg
    24061 ttctcctttc atgatatctt gctcattttt cagcgtattg tctttaaagt cggcgataaa
    24121 aggatgagga atgctctcta cccattcgtc ccaatgcttg tacattaaat cggtaacaat
    24181 tcggcctgtt gcttttggca aatcgggata tttgtcggca gcagttttac cgtatttaac
    24241 atcggcaata aaaagcactt tcgattcgtc gggagaaaaa agaaatccat tgatgcctcc
    24301 atcgcggttg gaaatttgtt tgcgctcact tccatcgatt ttcattgtcc ataactggtt
    24361 gcttccactt tcggaagaga ggaaagcaat gtgttcgcca tctttcatcc atcgggcctc
    24421 ggcttctttt gtggctgtat gagtgatttg atgcttgttt gttccatcaa cgttcataat
    24481 gaaaagttca gtatttcctt tgttttcggg aatactgtaa taggtaacac tgtaaagtat
    24541 tttggttcga tcaggagaaa tttgtactcc gccaactctt ccaaaagacc ataaaacttc
    24601 gggcgtcatg cgtccatttt cgatattggg aatctgtttc ccgattacgg aatgattttc
    24661 tttttcagtg catccagcac ctaaaacggc tattgccata agaatcgttt taaatttcat
    24721 ttgtagcttt caattgtaat ttttaagcgt aagtctaaat attttgaaca aaatcctgtc
    24781 tcaaatgcaa aagtaaataa tttccgaata tttataaatg ttctgaaaga ttttctggta
    24841 aactctgcaa ttttgaaaaa atatgatgag tttttggtaa atctcaaaaa aaaagcgtac
    24901 ctttgcaccc caatcaatcg taatctctga cagaataaaa gaagtctgta tatattgcga
    24961 atttacttaa aaaatcaagt gagatggact tattaaaaat tgcagaacaa tcttttgagc
    25021 aggaagtaaa aacttatcct gagtttagaa gtggtgatac catcacagta gcttatcgaa
    25081 ttaaggaagg aaacaaggaa agaatccagg aatttaaggg tgatgttata aaaatttccg
    25141 gtcatggagg aaagaagcat tttactgttc gtaaaatgtc gggaaatgta ggtgtagaac
    25201 gaatttttcc tatggattct ccgtttatcg aaagtattac ggtcaacaag catggtaaag
    25261 tgcgtcgagc caagctgtat tatttgagaa atctgaccgg caagaaagcc aaaattaagg
    25321 aaagaagagt ttgacattgg cttttcggca aagaaattaa aaacaggaga agtttgcttc
    25381 attgcagatt tctctttttt tgtttcttat cgtcattttt caaccgaaaa aagttttttc
    25441 tttttttccc cacaacagca tgacaattcc acatacaatc ggaaaaacgg ctattgaaat
    25501 cagcaacata tcgaaatgca taaccagcag attttctata aaattcaaca atatcccggt
    25561 atggatataa tggtacagca aaaacgaaat gcctgttgct ccaattagcc atacaatcca
    25621 ggaatgatct ttctctgttg gcaaccggcg catgactttt aaggaaaaac cattgtcggg
    25681 tatttccgtt ttatgggttt tgaaaaattc ctttaatttt tcttctgttt gttcattttt
    25741 tgtcataacc ttccttattt agataatcca tcaatttgtt ttttcccttt agcaggtagg
    25801 tttttaccgt tccttccgga caattcatga tttttgcaat ttctttctga gttttgtctt
    25861 ccatataatg caaaatgagc actgtttgtt cttctttttt taagtattgc aaagcgctgt
    25921 aaaagtccaa cttttcagca gaaaacgcat tgtcggactg atattgaagg tcaatttccc
    25981 taacgtcgag ttcggaaaat tgtttttgag tgcgcattga gtcctaaaac acattgtaag
    26041 cgatgcgata aagccatgtg gaaaagttgg acaaagcttg aaacgagtga atattcacaa
    26101 atgccttgat aaatgtttct tgtgccaaat cgtcgctcaa tgaggcatcg cccattgtca
    26161 gattcaaaaa gaaccggcga atgggcgact gatatttgcc aacgagtttt tcaaaagctt
    26221 tgttgtcgcc aagcaaggtg aattgtgtta tccaatgcag atcatccctg ttgctcattc
    26281 tgctcaattg gttgtttctc ttttgaccga tcgtaaaaat gcactactaa atatccaatg
    26341 cccacaaaag ttggaacaat tcccacaatc agcccatcat tattgtgatt gatgataaag
    26401 taaataagca aggcaagtcc cactgcagtc cataatacgc ctttttttaa gttggagttc
    26461 ggcttatcct ttttatcttt ctggaaaaaa tcttcaggaa caggctgtcc catttccaac
    26521 gccttcatgt acagttcaaa ttttcgtttc gactccttgt tttttgcagc ataaataatc
    26581 catatcagaa taatgacaaa aaggaaaggt gcaaccacaa tcatgaaaat ctggaatcct
    26641 gtaagtggca tgtcttctcg tgcattgcct tcaatttttt tcctttcgtt ttccagttcc
    26701 gcttttttca gttccagcaa ttgttcgggc gaaagtttat tgagcaagat ggaatctttc
    26761 tcccgctggg tcagattttc caaagcagcc ggcaatgacg gatgggaact ttcatcagat
    26821 ttgttctgcg caaaaccgat accggaaaac agtatcagcg aaaacgctaa taatacgaac
    26881 tttttcataa tgtttgaaat tttagaagta aatataatgt ttgtttatca gttagacgta
    26941 aaggaaacag tgttttgatt tgcaaagcaa tggaattaac ttttattagt ttttcatctc
    27001 ttttgttttt tatattttaa caaactagag gaaatttttc ttcgttggtg aacttgaacc
    27061 aaatagtttt ttatctgttt atataaagaa attttaattc tgaacaaatt gaaatgaaaa
    27121 cagaaaaatt tgatgtggtg ggaatgagtt gcgctgcctg tgcagcccac attgaaaaag
    27181 aggtcggaaa actggaaggt gttaaaaaag ttaatgtcaa tctgctgacc aacagtatgg
    27241 tagtagactt tgaggaatcg aacgtagata ctgataaaat tgaagaagcc gttgtagaag
    27301 ccggatacga agcacatgta aaatcatctg cagagcaggc tgcgagtcgg gaaacgaaaa
    27361 aaatagattt tgttcaaaaa gaacaacagg aaatgcagaa acgttggtgg atttctttcg
    27421 gtttctggtt accgttactg tatttgtcta tgggttccat gctgggttgg tatataccgg
    27481 cttttttcag gggagaagaa aatgcaatgg tttttgcttt tacgcagttt ttgttgactc
    27541 ttcccattgc aatgtcgaac gaaaagtatt tcagaaacgg tttcaaaatg ctttttaagg
    27601 ctgctccaaa tatggattca ctgattgcca taggttcttc ggcggccatt ttatatggaa
    27661 tttacggatt gtacaaaata gcttacggat ttggacatgc acaaccggag ttggtgcatc
    27721 agtttgctca cgatatgtat tttgagtcgg gagcaaccat tttgactttg attacgcttg
    27781 gaaaatatct cgaatcgcga tcaaaaagtc aaacatcccg cgccattacg caattgatgg
    27841 atttgtctcc aaaaacggca actgttattc gagacgggac agaagtagaa gttcctgcag
    27901 aagaggttag agtaggcgat atagtagttg ttcgtcccgg tcagcgaatt cctgtggatg
    27961 gcgttgtgga aagcggaagt tctacagtgg atgaatcggc actaaccgga gaaagtatcc
    28021 cggtactgaa acaaagcggc gaccgagtat tgtctgccag catcaacaaa tccggatata
    28081 tcacgctcaa agcaacgcaa gtgggtaatg ataccactct ttcgcatatt atcagcctcg
    28141 tggaagaagc ttcttcgtca aaagctccca tttccaagct ggccgacaga ataagccgta
    28201 tttttgtccc tgtggttatt ttcattgcct tgacggctgg tattgtttgg ttttttttgg
    28261 ggtattcttt cgatttttcc ttgtcggttg ccatttccgt tctggttatt tcctgtccgt
    28321 gtgctttggg acttgcaacg ccggttgcca ttatggttgg aacgggaaaa ggagctgaac
    28381 acggaatttt gataaaatcg gctgtttcgc tggagatggc acacaaaatt gatacggtag
    28441 tgttggataa aaccggaacg ttgacaatgg gaaaacctca acttaccgac atcattcctt
    28501 caccaaacat tacggaaaat gaattgcttc aaatagccgc ttcgctggag aaactttctg
    28561 aacatccatt ggcggaagca attctggaag aaacagacaa aaggaagttg gaaacttttt
    28621 cggtgaaaaa ttttacttct ctgttcggaa aaggaattgt agctgaagcc ggagaaaatt
    28681 tttttctgat aggaaacaga caattaatgg aagagcagaa aatagatgta cagaatttcg
    28741 ataatattgc cacgcaactg gctgaagaag gaaaaacgcc cttgtttgtg gcttccaggg
    28801 aaaaagtaat aggagtgatt gctgctgctg atccattgaa accaaccagc aaggaagccg
    28861 tgaaccaatt ccgttctttg ggactggagg ttgtcatgct gacaggtgat cacaaaaaaa
    28921 cggcggctgc tgttcagcgt aaattgggaa tttcaaccgt tttttcagaa gtgttgcctc
    28981 aggataaaga taaagtaata gtaaatctgc aaaaaggagg gaaaaaggtg gcaatggtgg
    29041 gagatggcat caatgatgca ccggctttga tgcgtgcgga tctgggaatt gccattggtg
    29101 ccggtaccga tattgctatc gaatcggcag atgttgtgtt gatgcgcaat gaccttatgg
    29161 atgctgtttc ggctattcga ctgagtaatg ccgtgatacg gaacattaag gaaaatctct
    29221 tttgggcatt tttctataat attattggta tacctttagc tgcaggtatt ttttatccgc
    29281 tgttgggctg gaaactcagt cccatatttg ccgcagcagc catgagtttc agttctgtta
    29341 cggtggtgct gaatgcactt cgacttcgct tttttaaacc ttcggaggtg aaatcttaaa
    29401 gttgaaaagt ctcaaaaaga atttcagata atttatagtt tgttgttgag tatagatgag
    29461 agttatttca attttgcaaa ttaaaaaata tccaataatc gatttatagg tatgtttaca
    29521 aactatttgc tgtgttgaat gacaggatga ctatcagact atttttgaaa tctttaattc
    29581 cgaacagaaa tttttatata gtaagatatc gattatagtt gcttctcatt gagattcatt
    29641 atctttgcag acgacaaaca aatgcaaaag agaccaaaat ggaaaatcag atcaatgggc
    29701 ctaaaaagca atacttcgaa cccatgcaca catgcgaaca tattctcaac cagactatga
    29761 ttcgcatgtt tggttgtggg cgctctcaaa atgcccacat cgaacggaaa aagagcaagt
    29821 gtgattatct tttgccgcag gcgcctacag aggaacaaat tcgggaactt tccgaccggg
    29881 taaatgccgt tatcgacagt catttgccgg taaccatcga atttctgaaa cgggaagaag
    29941 cttccggtgt agtagatttg tccaaactcc cggatgacgc ttccgacgtg ttgcggattg
    30001 tcagagttgg cgattatgat gcctgcgcct gcattggcca gcatgtgaac aatacttccg
    30061 aaatcggaca ttttgaaatt atcagccacg attatgaaga gggcaggtgg cgcgtacgtt
    30121 ttcgtctgat cgactaacaa atttttgcgt tatgttcagg aaagctttta aattcattaa
    30181 aaattgcgtc gttttattct ttctcatcag cattggatgg gtgattgtcg atcggtttgt
    30241 cccggtagtg gttacgcccc tgatgctcat tcgtaccgct gaatccgttt tctcgggcga
    30301 aacgccgaaa atcagcaaaa aatgggtgcc catcgacaaa atttcgcaca atatgattca
    30361 ggcagcagta gcagccgaag acgaccagtt tttgcatcat cacggttttt cctttgaagc
    30421 catcaatcaa gccattgaac acaataaacg aagtcgtcgc attcgtggag gcagtaccat
    30481 ttcgcagcaa acggcaaaaa atgttttcct ctggccgcag cgttcttacg tcagaaaagg
    30541 attggaagct tattttaccg tattgatcga acttttctgg agcaaggaaa gaattatgga
    30601 agtttacctg aatgtgatcg aaatgggaga tggaatttac ggcgtggagg ccgcttcgat
    30661 ggaatatttc ggcaaacatg cttccaaact cacaaaatcc gaagcggcac tcattgctgc
    30721 ctgtctgccc aatccccgac gtttcaaccc tgcccatcct tcaggatatg tattgagacg
    30781 gcaaggtcgc atcatggcca tgatgaacaa aatcgaaccg gtgcattttg aacgtccgga
    30841 atttaaattt ttaaaacata aaaaacaaaa gtaaccatga cccggcaaat cgaatttttg
    30901 gattggggat tgacagaata tgccgatgcg tggaaaaggc aggaagattt gtttcagctg
    30961 ctgcttcaaa acaaaaaaaa tgccgatttt aacacgcact atgttgtttt ttgccagcat
    31021 tttcccgttt atacgttggg aaaaaacgga catgaagaaa atctactgct caaccagatt
    31081 cagttgcaaa cccgcggcgc aagttttttc cgcaccaaca ggggtggcga tatcactttt
    31141 catgggccgg ggcaggtggt tggttatttt atttttgacc ttgaaaaatt gcaactggga
    31201 ctgaaagttt ttatttacaa actcgaagaa gtcatcatcc atctactcga acattatggg
    31261 ttaaagggta ctcgactgca cggagcaaca ggggtgtggc tggatgccga aacgccccgg
    31321 gcaagaaaaa tttgtgccat tggcttgcgg agctcgcatt ttgttaccat gcacggcttt
    31381 gcgctcaatg tcaataccga cctgaattat ttcaattaca tccatccctg cggctttgtg
    31441 gacaaaggcg taacttccct ccaaaaagag ttgttccaaa ccgtagagga agaagaagta
    31501 aaaaacttac tcaaaaaaca aatcgaaaaa gaatttttgt aatacaccta aatatttgtg
    31561 gttgtttttt ccatttcccg atggagctgt tcaagaattg ttgcaatctt tttttaatgg
    31621 atattggatt aaaaatagga agaataaatt tgtagaaata tttatttttt gtatcttttt
    31681 atttgttaat caataacatg agttacgtcc agagtagttt ttaaatccaa tgaacaagga
    31741 caaatcgttc tgttccctgc cagtttggat gaaaaagtac cacaagattc tcctgcacag
    31801 ttggttaatc aaatagtaga taatttggat attgctcaaa tcattgatac ttaaaaggga
    31861 ggatgaacaa gtgcctatca accgctaatg atgctgaagt atttttgtac agttatttga
    31921 acaatattta ttcgattcg
//
LOCUS       DAOSIB010000016        31216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_1651_length_31216_cov_10.264497, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000016 DAOSIB010000000
VERSION     DAOSIB010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 31216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31216
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1651_length_31216_cov_10.264497"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..471
                     /locus_tag="PLD44_02685"
     CDS             226..471
                     /locus_tag="PLD44_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOF98277.1"
                     /translation="MNIYVGNLSYKVRENDLQGVMEEYGQVSSCKIIKDRETGRSKGF
                     GFVEMSDDTAATKAIEELNGAEFDGRTMVVKEARPKN"
     gene            577..1455
                     /locus_tag="PLD44_02690"
     CDS             577..1455
                     /locus_tag="PLD44_02690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOF98278.1"
                     /translation="MSSLKGVFKNRKIFSQLLLLLFLSGLFFYIGLAFWMVISAGRRE
                     DAQAIRVLQLIQSIGLFLIPPFIFAYLVSDRKPTEYLSLSQKINLKDVMYVILIMFIA
                     IPFVNFLTSLNQQLVLPEVLAPVEEWMKQTEEEAALLTKQLLQVDNIGGLFANLILIA
                     LIPALGEELYFRGALQNVIGRGKGTHFAIWMTGFLFSAFHLQFYGLLPRWLLGVFFGY
                     ILVWSGNIWLPVIAHFTNNAIAVIFYYFSGQNNEVVNIDAVGTNDSWWMGVISLILTI
                     LLVFRLKSRIPKKQHI"
     gene            complement(1452..1847)
                     /locus_tag="PLD44_02695"
     CDS             complement(1452..1847)
                     /locus_tag="PLD44_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HOF98279.1"
                     /translation="MWVLIIILISVGLVLMTVTYFSRKGKREEATVQVRENSECCGAH
                     ALCERENLLNMENEIEYYDDEELDALSGKSPTQFSDKEIEQLSEVFYSLQESDVAGWL
                     RSLQMRQIELPLFLREEALMIVGERRNAC"
     gene            2181..3578
                     /gene="nhaA"
                     /locus_tag="PLD44_02700"
     CDS             2181..3578
                     /gene="nhaA"
                     /locus_tag="PLD44_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HOF98280.1"
                     /translation="MIDHHSKRARIYIFLRNFKWETNPGIVMMVVAIATLVIANSPLK
                     EWYQSFWNIEFLLGTNSFNLFSHHGTPLTLLSFINDCLMAVFFFSIGLEIKREVLIGE
                     LSSFRQSILPMIAALGGMIVPVLCFFVTGRIQNLSPDEMRGMFIPMSTDIAFSLAVVS
                     LLGKKIPVSLKVFLLALAIIDDIGGIIVIALFYSDFNAESVIYLLYAAFFVIVLALGG
                     KLRINGKLFYAFFGIIVWYLFLQSGIHPSIAGVVVAFTVPAHPRINIRTFIRQLRRDI
                     DLLESVSPKPTKEEIILSNTQIKYLSHIENAADQVISPLQDFEDNLYVLVNFIILPLF
                     AFANAGVVINLSDFYLSGIALAIIIGLVLGKTLGIFSFTWLTIKSGLSRMPNGMNWHS
                     LFGLSLVGGIGFTVALFLTALSYPVGSEILNQAKLGIIAGSLISGFLGYYYLKFSINR
                     GEKNNYRLKKNSQAS"
     gene            3688..5205
                     /gene="guaA"
                     /locus_tag="PLD44_02705"
     CDS             3688..5205
                     /gene="guaA"
                     /locus_tag="PLD44_02705"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOF98281.1"
                     /translation="MEKIIILDFGSQTTQLIGRRLRELNEFCEILPYNKFPEQTKDIK
                     GIILSGSPFSVYDPKALQIDLNKIRGKFPILGICYGAQYIVHSAGGKVQPGNTREYGR
                     ASLTYVDATDPLMSNIPVGSQIWMSHSDSITEVPHNFIQIGSTQDVQIAAFRIEGEKT
                     WGVQFHPEVFHTTEGLLLLENFLNICDCRRNWTPASFIETTIQQLKEQLKDEKVVLGL
                     SGGVDSSVTAVLLNRAIGKNLTCIFVDHGLLRKDEFREVLTNYEHLGLNVIGVDAKER
                     FYAVLRGIADPEKKRKIIGAEFINVFNEEAKKIENVKWLAQGTIYPDVIESYSISGNT
                     IKSHHNVGGLPEKMHLKLCEPLRLLFKDEVRSVGIELGMTPQLIKRHPFPGPGLAVRI
                     LGEVTPQKVAILQEADYIFIQELRKWDLYDKVWQAGCVLLPIQAVGVMGDERTYENVV
                     ALRAVTSTDAMTADWAKLPYDFLAKVSNEIINKVKGINRVVYDISSKPPATIEWE"
     gene            5330..6118
                     /locus_tag="PLD44_02710"
     CDS             5330..6118
                     /locus_tag="PLD44_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOF98282.1"
                     /translation="MIIQTSMIDTHAHIYSEEFDADREKVIERALKRGIEHIILPNID
                     LQSLQPMLQLEASHPDICHAAIGLHPVSVKENFAETLNFLKFKLQQHNYIAIGEIGID
                     LYWDKTYQNEQIEAFCQQIEWALEWELPVIIHVRDSFKETMQALEPYRNKKLRGVFHS
                     FTGTIEEANQIITFGGFKIGINGILTFKNSELSKTVSQIETEYLVLETDAPYLSPVPF
                     RGKRNEPAYLSYICEQLALILGKTSEEVEFITTQNAKKLFKLQP"
     gene            6213..6302
                     /locus_tag="PLD44_02715"
     tRNA            6213..6302
                     /locus_tag="PLD44_02715"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6247..6249,aa:Ser,seq:gga)
     gene            6382..7191
                     /locus_tag="PLD44_02720"
     CDS             6382..7191
                     /locus_tag="PLD44_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOF98283.1"
                     /translation="MKKVFAILSIVAIFSFGSALHASAQDTSAPQTEQTAGNDTAVAA
                     AQAMEQVEAPAPAQGGGMHKALKQKFIEGSPAWMAPIALCLIIGLALCIERIIYLNLA
                     STNTKKLLEDIDQAWAKGGVQAAMEVCRNTRGPVASIFYQGLSRYDEGIDVVEKAVAS
                     YGGVQLGLLETNLTWISLFIAISPSLGFLGTVIGMIEAFDRIQQVGDISATVVAGGIK
                     VALLTTVFGLIVAMVLQVFYNYILTIIENMTNDMEDSSISLLDIIVKHSKK"
     gene            7215..7700
                     /locus_tag="PLD44_02725"
     CDS             7215..7700
                     /locus_tag="PLD44_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98284.1"
                     /translation="MSKFMQKIPIISAIFLVSVSVILVLLIYVGGTSGSINNSAGEAM
                     SVPRFTDALIYWSYFLVGLAVVITLGFVVKNFFQSSKQSSSSIFKSLLPIIGFILLFV
                     VSWLLGSDQELKIIGYEGAENVGFWSRFTDMMIFPIYVMAIGIILTIIGTSIYSSLRK
                     D"
     gene            7752..8354
                     /locus_tag="PLD44_02730"
     CDS             7752..8354
                     /locus_tag="PLD44_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOF98285.1"
                     /translation="MSKKKRKVGQINASSMADISFLLLIFFLVTTSMNVGTGIARRLP
                     PPLPPDQKPPDMDIKKRNIFIVRINSQNQIMAQGEILDIKQLRYKTKEFIKNENNDPN
                     LPELITENIPLLGEVTYTKNHVISLQNDVGTQYQAYIDVQNELIAAYNELRDEFAMQR
                     FGKKYNELSQELQEAVQKIYPQKISEAEPKNYGGVKSGKN"
     gene            8341..8811
                     /locus_tag="PLD44_02735"
     CDS             8341..8811
                     /locus_tag="PLD44_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOF98286.1"
                     /translation="MARIRKSEKREMPALSTSSLPDIIFMLLFFFMAVTTMKEVTYKV
                     RIDPPKATEIQKLENKSLIRYIYVGKPLEQYQRIYGSETRIQLSDMFADISEIEEYII
                     NERSAMKEEDQNLMSVCIRADKDTHMGIITDIKQALRRAYALKIIYAANQRTQY"
     gene            complement(9038..10081)
                     /locus_tag="PLD44_02740"
     CDS             complement(9038..10081)
                     /locus_tag="PLD44_02740"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOF98287.1"
                     /translation="MKKIIVSFLMASVISSVLCQSSLTLKENESVLIYSLPMTELCIK
                     VDVEKITRKPGVFYRYSERYLATSDIITEEKTVYSLKDIQVTSNAVPDPSKTYAVRVD
                     KNSPFVSISVDQNGLLCGINVPPPTSLPELSVFAPTEAKENLSNNVILPLGEEYMLVG
                     SVAKLAEGAAKQIYRIRESRLNLLTGDVDHFPSDGTSLSAILNELDNKEKELTELFIG
                     STTKEIQKHTLYLRPQQALNNEVLFRFSVLKGLVPADDLSGVPYYISVIPTKSPTILD
                     NPKQKAPKEYINFVIPSATLVTVSDGKSTFFSQQFLFPQFGIVIPLSSSMIKGNDKIV
                     IIDPATGRLLSIF"
     gene            10526..11293
                     /locus_tag="PLD44_02745"
     CDS             10526..11293
                     /locus_tag="PLD44_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOF98288.1"
                     /translation="MGKIISLANQKGGVGKTTTAINLAASLASLGKKVLLVDADPQAN
                     ASSGLGIEIQSLKYTIYECLIDGVSPQQAIHGTEIENLSVLPSHIDLVGAEIEMLNME
                     EREKVMQHLLRPLKPDFDFILIDCSPSLGLITVNALTASDSVIIPVQCEYFALEGISK
                     LLNTIKIIKSKLNPSLDIEGFLLTMYDNRLRLANQVYAEVKKHFQNMVFENVIYRNVR
                     LSEAPSYGKPVMLYDPESKGSSNYLDLAKELIDRQKS"
     gene            11341..12222
                     /locus_tag="PLD44_02750"
     CDS             11341..12222
                     /locus_tag="PLD44_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOF98289.1"
                     /translation="MVKKSALGRGLGALIDTDDIYTSGSSSISEVDVDLIFPNPNQPR
                     SHFDEESLAELASSIRELGVISPITLRKNDDGTYLIIAGERRYRASKMVGLKSIPAYV
                     KTAADEQVVEMALIENIQREDLNAIEVALAFYRLLEEYHLTQEQLSERVGKKRATIAN
                     YLRLLRLPAEIQVGIKDKKIDMGHARAILGLEDPAVQLQLYEAILENFYSVRKVEEMV
                     RLYAETGSFEEKHARVSKPNPVRKLKEYEELKNQLSKTFGTKVQFSCNEQGKGKISIP
                     FSNDEELSRIMGLLDKI"
     gene            12235..12912
                     /locus_tag="PLD44_02755"
     CDS             12235..12912
                     /locus_tag="PLD44_02755"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOF98290.1"
                     /translation="MKRFFLITAEIWFISLFGIVLNAQNVNEKREIVQDSINRLPEKS
                     LQKESQVSIDTVQKPVYMPNPSKIVWMGAVVPGWGQILNRKYWKLPIVYGGLLGCYYA
                     YSWNASRYNSYHTAYLDIIDSDPTTNSFLDILPAGYTLESIGGTGRYTTILKSGQDQF
                     RRQRDLSVIVSVAFYALTLVDAFVDAQLYGFDISPDLSLEIKPIYLKDNITTSGGLGM
                     QCRFKLK"
     gene            12938..14215
                     /locus_tag="PLD44_02760"
     CDS             12938..14215
                     /locus_tag="PLD44_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOF98291.1"
                     /translation="MNLKFSIIVVSFICFSINSVISQAAKDLPEDTLSSKIDSIIGIP
                     DAYGYALDHLLHLWALQRMDTTECNSGEPPAPVPDSLYKYRLSQIPCLIELPYNSIVR
                     SFIELYTVRKRTQTEYMLGISNYYFPVFEQALDANKLPLELKFLPIIESALHPTAISR
                     AGAAGLWQFMIPTGRMYGLEINTLVDERLDPRKASYAAARFLKDLYAIYGDWNLVIAA
                     YNCGPGNVNKAIRRAGGKTDYWAIYPYLPLETRGYVPIYIAATYTFHYAEEHNLCPAV
                     VNIPALIDTFMINQRIHLEQISSVLNIPIEELRWLNPQYKKDIIPGDLKPYVLTLPVN
                     YSSIFMERLHEIASYKADELINNRRNEIEAFRSTSSSNLGASKNVTYHKVKKGETLSG
                     IAAKYGVSVSNLKKWNNIKGSKITIGQRLKIIK"
     gene            14224..14544
                     /locus_tag="PLD44_02765"
     CDS             14224..14544
                     /locus_tag="PLD44_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOF98292.1"
                     /translation="MEKVYYSISEVAEMLKISPSNLRFWEKEFSQLKPKRSNKGTRTY
                     TKEDIEIIKMIHLLVNEQKLTLQGAKRKLSLKKETVSKQQELVERLQNVRRELLMISK
                     ALDE"
     gene            14568..15638
                     /locus_tag="PLD44_02770"
     CDS             14568..15638
                     /locus_tag="PLD44_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOF98293.1"
                     /translation="MKKTNFCLGLIAALILMSASVSAEKKPKNVILMIGDGMGLAQMY
                     SAMTANNNQLQLERCTYTGFSKTYSSDHYTTDSGAGGTAIACGVKTKNGMIGMTPDSV
                     KIESILELSERNQLSTGIVVTCAVTHATPASFIAHQISRNMYEEIAGDFLQTDVDVFI
                     GGGLKYFNNRKDQINLVEKLKKNNYQIAYSLDEAVKVKSGKLAGLFYENHCPPMPERG
                     DMLPKSVMAAIDILDNNKKGFFLMVEGSQIDWAAHDNDKKQIVKEMLDFDETIGKVLD
                     YAQKDGNTLVIITADHETGGMTILNGKFGSDEMDAHFSTKNHSGVPVPIYAFGPGAEQ
                     FTGIMENTAFKGKIEKLLDLKK"
     gene            15735..16842
                     /gene="prfB"
                     /locus_tag="PLD44_02775"
     CDS             join(15735..15797,15799..16842)
                     /gene="prfB"
                     /locus_tag="PLD44_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445733.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOF98294.1"
                     /translation="MITIEQLKDVTERTEALRRYLDVDNRQIQIEEEELRTQEPNFWD
                     DPKKAEAQMRKLKELKNWVTGYQEVKTAADELELAYDFFREGVIEEQELDETYRKALQ
                     LIEQLEMNNMLSHEEDQLGAVLKIVAGAGGTEAQDWAEMLMRMYQRWAERKGYKWEIT
                     NFQPGDEAGIKSVTIQIEGERAYGYLKSENGVHRLVRVSPFNAQGKRMTSFTSVFVVP
                     LIDDSIEIEINPANLSWDTFRSSGAGGQNVNKVETGVRLHYTFKHPVTGEQSEIVIEN
                     TESRSQFDNKDNALRLLKSQLYELELQKRRAEQEKVESTKKKIEWGSQIRSYVFDDRR
                     VKDHRTNYQTSNVQAVMDGDIDEFIKAYLMEFPD"
     gene            17261..18922
                     /locus_tag="PLD44_02780"
     CDS             17261..18922
                     /locus_tag="PLD44_02780"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOF98295.1"
                     /translation="MKETKYIFVTGGVVSSLGKGIIAASLGKLLQSRGFKVTNQKLDP
                     YINIDPGTLNPYEHGECYVTVDGHEADLDLGHYERFLNIETHRANNITTGRIYQNVIE
                     KERRGDYLGKTVQIIPHITDEIKRNIKLLGSKGDYDFVITEIGGTVGDIESLPYIESV
                     RQLRWELGKNCLIVHLTYVPYISAAKELKTKPTQHSVKALQEQGVQPDILVLRTEHNI
                     SSDLRKKVALFCNVEPNSVMQSVDVSTIYRVPLMMYDEKLDEVVLRKMGFDLLKIPQA
                     NLSKWIQFVEKLENAKEEVHIALVGKYVQLPDAYKSIIESLLQACAYNDRKLKLDLIL
                     SDKLTEENIEKKLEGVDGVIIAPGFGQRGIEGKLVTIKYTREKNIPTFGICMGMQCMV
                     IEFARNVLGYADANSTEIDPTTTHNVVDIMEEQKNILNLGGSMRLGAFPCKLKKGTKV
                     FKIYGKENISERHRHRFEFNNYYKEEFDKAGMICSGINASSNLVEIVEVPSHRWFIGV
                     QFHPEYSSTVINPHPLFISFIKAAIEYRNESKEKTQNSKFKTAIK"
     gene            18979..20883
                     /gene="yidC"
                     /locus_tag="PLD44_02785"
     CDS             18979..20883
                     /gene="yidC"
                     /locus_tag="PLD44_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOF98296.1"
                     /translation="MDKNTITGFLLITVIIVVFALLNRPNQEMIEKQRRYNDSIAQIE
                     KAKAESETAIQEVNSLLSDSIKTTLDSSAVIRDAYGDFSVAALGKEEFITLENSVLKL
                     TFTNKGARIYAAELKDYRRHDSLPLILFQGSEANMGFTLITNNNRILNTANLFFEVLL
                     PIEKDTLGNQILVFRLKTTGEGYMDVVYTLPPDNYMFSFSIKANKMSNVMPAGTNFLE
                     MQWTTRLRQQEKGRQFESRYSNIQYKYLSDDVEKLSESKNDSKQITNKLKWIAFKDQF
                     FSNVLIAEDAFTSSFLESKVEPEDSPYLKSYRADMTIPFDPTGKTSTNFRMYLGPNKF
                     NILASYDKGVAKEEKLQLHKLIPLGWGIFGWINRYMIIPMFNFFSKYIGNYGIIILLM
                     TIIIKIIIFPMTYKSYTSSAKMRVLKPEIDEIYNKIPPEKAMERQKAVMDLYSKVGVS
                     PMSGCLPALLQIPILFAMFSFFPASIELRQESFLWAKDLSTYDSIISWNAYIPIISKY
                     FGNHLSLFCLLMTISSIISTKLTMTTTPTTNQPGGNLMKWMMYLMPVMFMFIFNNYSA
                     ALSYYYLLSTLITIIQTFIIRSMVDEKKLLAQLHAKRNQKQPAKKKSSWIERMEKMQR
                     EQQKALKNKR"
     gene            20964..21974
                     /locus_tag="PLD44_02790"
     CDS             20964..21974
                     /locus_tag="PLD44_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98297.1"
                     /translation="MTKKIKFFILFFLIIFLFSCQNHNRFRINTKKNEVEVRIRRFDK
                     DLINVDTTNLKAEIRKIYKDYPVFFPVFINEILNVAPSDTDSVAKLIGDFLCDAAFQK
                     VNKEVLKTFADVHDIEKQLSIAFTYMHHYFPEIRLPEIYFFVSGFNRSIIIYPSLIAV
                     GADLYLGADYPLYKSFTYEYLTFNMRPESIAPDVVSTLLFSYFPMESENNRLLDNMLY
                     NGKILFLLSVFMPDVEPSVLMGYTREQWQWCKRFEKEIWAKIIEDKALFSSDMRLINS
                     YLDEAPFTAPVSQDSPGRVGTWIGWQIVNSYMKKNNNVSLRQLANENNYQLLLQKSDY
                     RP"
     gene            complement(22041..22208)
                     /locus_tag="PLD44_02795"
     CDS             complement(22041..22208)
                     /locus_tag="PLD44_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOF98298.1"
                     /translation="MAYVITEDCIACGSCITECPVEAISEGDIYVIDPDVCTDCGVCA
                     DVCPSEAIIPA"
     gene            complement(22586..24193)
                     /locus_tag="PLD44_02800"
     CDS             complement(22586..24193)
                     /locus_tag="PLD44_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOF98299.1"
                     /translation="MDRNKKNYLAPLIVFLAVIFGLILGNLLAQRAFFSSSPNIFFGF
                     GNASKVDELLQIINTNYVDTLNLDEMTENVMQDIIAKLDPHSVYIPAEDISLANSELE
                     GSFSGIGVQFNIQNDTVMIISVISGGPSETAGLMPGDRIVEVNDTTFVGKSINNEKVM
                     KKLRGPEGTKVKLGIKRNQTKEVLYYEITRGKIPVNSVDIAYIVQPSVGFIKVSKFAE
                     TTYGEFLNAIAKLKNEGADKFIIDLRGNSGGFMDQSINMINEFLPANRLIVYAQGKAY
                     KRFEAKSDGKGSCTREPLVVLVDEFSASASEIFAGAIQDNDRGTLVGRRTFGKGLVQQ
                     QFNLSDGSAVRLTVARYYTPSGRSIQKPYKLGNSEEYEKEFINRLLHEINDSIFHPDS
                     LRYKTVGGRIVYGGGGIMPDVYVPLDTSEFTPYFNNVVNYGYVYQFAFQYSDKNREKL
                     KKIKNWHEMDTYLNSQSLLDQFVKFVSEKGIQANKKEINISKNLLLTQIKGYISRNIL
                     GDEGFYPLLYRSDKTVLKALDVINSKQ"
     gene            24651..25028
                     /gene="gcvH"
                     /locus_tag="PLD44_02805"
     CDS             24651..25028
                     /gene="gcvH"
                     /locus_tag="PLD44_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOF98300.1"
                     /translation="MKVPENLKYTTEHEWIRVQGNTAWVGITDFAQHELGEIVFVEVE
                     TLGENIHAGEVFGTVEAVKTVSELFLPISGEILEFNKELENKPELLNDDPYGEGWIVK
                     IRVADPSELEKLLSAEQYVKLIA"
     gene            25060..25566
                     /gene="purE"
                     /locus_tag="PLD44_02810"
     CDS             25060..25566
                     /gene="purE"
                     /locus_tag="PLD44_02810"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOF98301.1"
                     /translation="MKARASIIMGSTSDLKIMEKAAKLLDEFEIPFEIHALSAHRTPK
                     EVEDFALHAEKRGIEIIIAGAGMAAHLPGVIASMTTLPVIGVPIEASLNGMDALFSIV
                     QMPPGIPVATVGINAAQNAAILAVQILALKDEKINSKLKLYKENMKQKIVEANNQLKE
                     IKYTYKTN"
     gene            complement(25785..25970)
                     /locus_tag="PLD44_02815"
     CDS             complement(25785..25970)
                     /locus_tag="PLD44_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98302.1"
                     /translation="MMKAFSHRTKVRNLGNITTKLETKRYSVGYREKGEIGEKTKNKM
                     SFSSGDGVIKWFSLPSG"
     assembly_gap    26205..26304
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            26315..28534
                     /locus_tag="PLD44_02820"
     CDS             26315..28534
                     /locus_tag="PLD44_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOF98303.1"
                     /translation="MTTYPSQSVFSLYRRMVTSEKNMFSFQERKTIKQILKEYYLLHP
                     QQLTLPGHSIEDKLYIIQILLNEIGLGKASVLSVLFMEPIKNNYLTVSEIEQKFKTPL
                     DSIIGGMQRVEELYERSFSLETENFRKLLLTFAQDVRVILMMIAERLYFMRILDKFPA
                     DMQQSMARECSFLYAPLAHRMGLYSIKSEMEDLSLKYTNREIYREIAQKLNETKRSRD
                     KYIEEFITPLKEKLGKEIKYPFEIKGRTKSIYSIYSKILKQNTPFENIYDLFAIRIIL
                     DVPLEKEKMICWQVYSIVTDMYQPNPKRLRDWLSIPKSNGYESLHITVLGPQNKWVEI
                     QIRTTRMDEIAEKGLAAHWKYKGIKGESGMDEWLKNIRDILENPDLNAVDFMDEFKLN
                     LYDQEVFVFTPAGDLHKLPKGATVLDFAYLIHSNLGSKCIGAKINKERNVPIKYVLKN
                     GDQVEILTSPSQKPNPSWLNIVVTSKAKSKIRQALKDIEFKDAEIGREILLRRFKNWK
                     IELEEGKLSRLAKKKGYKTVSDFYQAIAHEKIDMLLIRDAYLDLDRHETGAEQAEPRS
                     AETFSKETPAEELSSKEDVLIIGQNLKNVDYTLAKCCQPIYGDDIFGFVSVGGGIKIH
                     RTTCPNAPQLISKYGYRIIKAQWAGRSGGTQYPITLYIVGKDDIGIVTNITSLISKEK
                     NILLRSISVDSNAGVFQGNLTIMVNDANDLDSIIKKIKQVKGVLNISRTKSLTQNNQ"
     gene            28548..29147
                     /locus_tag="PLD44_02825"
     CDS             28548..29147
                     /locus_tag="PLD44_02825"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOF98304.1"
                     /translation="MFSGIIEEAAPVVALKKEAGNLHITMTCSFVNELSVDQSVSHNG
                     VCLTVVSKTPDTYTVTAIRETLEKSNLGLLQEGSKVNLERSLLLTERLDGHIVQGHVD
                     TTAVCTDISEADGSWYFTFQYDVDRELAKRGYVTVDKGSVAVNGVSLTVCNPTENTFQ
                     VAIIPYTYEHTNFHQIVVGTTVNIEFDIIGKYISRLMKL"
     gene            29234..30391
                     /locus_tag="PLD44_02830"
     CDS             29234..30391
                     /locus_tag="PLD44_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOF98305.1"
                     /translation="MENFRKQIKISFFNPIIHFLPLLLFLVTAMYAGRTVANYVSIFV
                     LLVSAVYVFIAYNQIFIWFFLSCVIFLAVNIVGSIIRLPIIFLSFRPLLDEALTLVFL
                     GGMILLQKPIKKGIMALIPKTLPMNNNLDEYFGFIRTIFLIILGYVLATGFFYAFPTQ
                     RETAYLQLVQAIYVGILFFVLVFESLRVQIIRARLLKEEWLPIVNLQGKIIGSVQHQA
                     SLGEKRKFMHPIIRVHIIQDGMILLRKRPADDLSYPLHWDTAISNHIRMGETVEDCIK
                     RTAQELYGLTELKSFFLSNYITHTDFEQHYAFLFIACKMNKIDLQSEKVKQAKWWTLQ
                     QIEENLQEGIFTENFLIEFDLLKKSGVLENNICDCACKLKETIFKAPHLFR"
     gene            30413..30988
                     /locus_tag="PLD44_02835"
     CDS             30413..30988
                     /locus_tag="PLD44_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98306.1"
                     /translation="MMRLYFILLLIAFVSCGGHSNSEFKLQNSFQIKLQDSICINAKD
                     CFYFSTDSNLYRIFMYFSNAELKEKKIIDTVDFSPYKSKIHSFQSENNESYVVLWETE
                     YEFYPLIYVYFITAGKIVKIGELLISLPCQSCESFEYPLKDIRMLQKGKNIEISFLKD
                     VNYKPSNGNEWHLYKAGMLKCIFNAETNKLK"
BASE COUNT        10020 a         5348 c         5972 g         9776 t
ORIGIN      
        1 gaaaaaatta aaatttttta tatactgaaa taatttttct attttttttt gttatttgaa
       61 aaaaaagcat ttcctttgta tccgattaca agaagtgtat ctccctcgat cggttatttt
      121 caactttcca tttctctgga cggcaatctt taacctcgta tgtttgtttt actcctcttt
      181 aatcgcaaat tatattaatt tctaattttt aattttttta tttttatgaa catttatgtt
      241 ggtaacctga gctacaaagt tcgggaaaat gatcttcaag gggttatgga agaatacggt
      301 caggtatcat cctgcaaaat cattaaagac cgtgaaaccg gaaggtcaaa aggattcgga
      361 tttgttgaaa tgagtgatga cactgcagca acaaaagcaa ttgaagaatt gaatggagcc
      421 gaattcgatg gaagaacaat ggtagttaaa gaagcacgtc caaaaaatta agcttttttt
      481 aaaaatcgaa atggagaact tctattgtcg caagacaatg gaagtttttt gtttttaact
      541 gttttcacga atgattaatg atatatcgac agaagtatga gctcattaaa aggtgttttc
      601 aaaaatcgta aaattttttc tcaattattg ctgttgttgt ttttaagtgg cttgtttttt
      661 tacattggtt tagctttttg gatggttata tcggcaggaa gaagagaaga cgcccaagcc
      721 atccgagtat tacaactgat acagtcaatc ggattgtttt tgattccacc atttattttt
      781 gcatatttag tgagtgatcg taaaccgaca gaatatctca gtttatctca aaaaatcaac
      841 ttaaaggatg tgatgtatgt gatactaata atgtttattg ctattccatt tgtcaatttt
      901 cttacatctc ttaatcagca actggtttta ccggaggtac ttgcacctgt ggaagaatgg
      961 atgaaacaaa cagaagaaga agctgcattg cttaccaaac aacttttaca ggtcgacaac
     1021 ataggtggat tgtttgcaaa tctaatatta attgccttaa ttccagcatt gggagaagaa
     1081 ttgtattttc gtggtgcttt acaaaacgtc ataggtcgtg gcaaaggtac ccactttgcc
     1141 atctggatga ccggtttttt gtttagcgca tttcatttgc agttttatgg ccttttgcct
     1201 cgatggttgc tgggagtgtt ttttggatat atcttggtat ggagtggaaa tatatggctg
     1261 ccagttattg cgcattttac caacaatgca atagcagtga tattttacta cttcagtgga
     1321 caaaacaatg aagtagtaaa cattgatgct gttgggacaa atgatagctg gtggatgggt
     1381 gtaataagtt tgattttaac cattttgctt gtgtttaggt taaaatcaag aattccaaaa
     1441 aaacagcata tttaacaggc atttcttctt tcgcctacta tcattaaagc ttcctctcgt
     1501 aaaaataaag gtaattctat ctgcctcatt tgtaaactgc gtaaccaacc tgccacatcg
     1561 ctttcttgca aactgtagaa aacttccgaa agctgttcta tttctttatc ggaaaattgt
     1621 gtcggcgatt ttccactcaa agcatcaagt tcttcgtcat cataatattc tatttcattt
     1681 tccatgttta agagattttc tctttcacaa agagcatgtg ctccacagca ttctgaattt
     1741 tctcgaactt gtaccgttgc ttcttctctt ttgccttttc ggctaaaata ggtaactgtc
     1801 attaaaacaa gtccgactga aatcaaaatt attatcaata cccacataat tgtttgtatc
     1861 ctttcttagt tgtttataaa tatacaacaa attttttgac tgtaaagtta gtaataaaac
     1921 tgaaaaaatg tttgttaaat tgtgtatgaa ttttcggtcc cacttatgtt atatttatcg
     1981 tacactaagt tatacaaaaa atattttttt gttttctctt taatattttc ttacctttgg
     2041 ataattttta cagaatgatg tcccactcaa gggtatagat tttgatatct ggtattataa
     2101 attttttttt gtagaatttt gatgcatatt taccattaat ctaaggattt tactcataga
     2161 tgcttaattc ctgaataaat atgattgatc accattcgaa aagagcaaga atatatattt
     2221 ttttaagaaa ttttaaatgg gaaactaatc cgggtattgt catgatggta gttgccatcg
     2281 caacccttgt tattgccaat tctccattga aggaatggta tcagtcattt tggaatatag
     2341 aatttttact tggtacaaat tcattcaacc tttttagtca tcacggtaca ccccttaccc
     2401 tgttgagttt tatcaacgac tgcttaatgg cggttttctt tttttctata ggtttggaaa
     2461 taaaacgaga agttctgatt ggagaacttt catctttcag gcagtcgatt ttacctatga
     2521 ttgcagcttt gggaggaatg attgttcccg ttttgtgctt ttttgttacg ggtcgaatac
     2581 agaatctttc gccagatgaa atgagaggaa tgtttatccc gatgtcaacc gatatagctt
     2641 tttcgttggc agtggtatcg ttgttgggaa aaaaaattcc tgtgagttta aaggtttttt
     2701 tgcttgctct ggcgattatt gatgatattg gtggtattat agtaattgct ttattttata
     2761 gcgatttcaa tgccgaatcg gtaatatatc ttctttatgc tgcatttttt gtgattgtcc
     2821 ttgctcttgg aggcaaattg cgtatcaacg gaaaactatt ttatgctttt tttggtatta
     2881 ttgtatggta tttgttcctt cagtcgggta ttcatccttc cattgccggc gtagtagttg
     2941 cttttactgt tcctgcccat ccgcgtatca acatcagaac ctttatacgt cagctgcgca
     3001 gagatattga tttacttgaa agtgtttcac ctaagcccac gaaggaggaa ataattctca
     3061 gcaacacaca aattaaatat ctttctcata tcgaaaatgc agctgatcag gtaatcagtc
     3121 cgctacaaga ttttgaagac aacctctatg tattagtgaa ttttattatt ttgcctcttt
     3181 ttgcctttgc caatgccggt gtggttatca atctgtccga tttttatttg tcgggtattg
     3241 ctctggccat catcataggt ttggtactgg gtaaaacatt gggaatattt tcctttacat
     3301 ggcttaccat caaatcgggc ttatcgcgta tgcccaatgg catgaactgg cacagtctgt
     3361 ttggtttgtc attagttgga gggataggtt ttacagttgc attattctta actgctctat
     3421 cataccctgt cggaagcgaa atacttaatc aggcgaaatt aggtattatt gcagggtctc
     3481 ttatttcggg ctttttgggt tactattatc tgaagttttc gatcaacagg ggtgaaaaaa
     3541 acaattatcg attgaaaaaa aattcacagg cttcataatc atgtgtttaa acatttgtaa
     3601 gatgcctttt gacaaaaaaa taaaatactc aagggagttt gccaaatatt ttataaagca
     3661 taatttgtaa ctatccgaac tatttttatg gaaaaaatca ttatactcga ttttggttcg
     3721 caaaccactc aattgatagg tagacgttta agggaactta acgaattttg tgaaatactt
     3781 ccctacaaca aatttcctga acaaacaaaa gacataaaag gtataattct ttccggaagt
     3841 cctttttcag tttatgatcc aaaagcacta caaatcgatt tgaataaaat tagagggaaa
     3901 ttcccaattt tgggaatttg ttacggggca caatatattg ttcactcggc cggagggaag
     3961 gttcagcctg gaaatactag agaatatgga cgagcttcgc tcacctatgt ggatgcaaca
     4021 gacccattaa tgtcaaatat tcctgtcggt tctcaaattt ggatgtccca tagcgattct
     4081 ataaccgagg ttccgcataa ctttatacaa attggaagta cgcaggatgt acaaattgca
     4141 gctttccgta tcgagggaga aaaaacctgg ggtgttcaat ttcatcctga agtatttcat
     4201 actacagaag gcctattact cttagaaaat tttttaaata tttgcgattg tcggcgaaac
     4261 tggactcccg cttcatttat agaaactacc attcaacaat taaaagaaca attaaaagac
     4321 gaaaaggtgg tactgggatt gtcgggaggg gtagattctt cggtaacagc cgtgttatta
     4381 aaccgagcca ttggaaaaaa tttgacctgc atttttgtcg atcatggttt gctcagaaaa
     4441 gacgaatttc gtgaagtact cactaattat gaacatttgg gtttaaatgt tattggagtc
     4501 gatgccaaag aaagatttta tgccgtactg aggggaatcg cagaccctga aaaaaaacgt
     4561 aaaattattg gagccgaatt tataaatgtg tttaacgaag aagctaaaaa aattgaaaat
     4621 gtaaaatggc tggcacaagg aaccatttac ccggatgtta tcgaatccta ttccatttcg
     4681 ggtaatacta ttaagtctca ccacaacgtt ggcggattac ctgaaaaaat gcacttaaaa
     4741 ttatgtgaac ctctgcgttt actttttaaa gatgaagttc gcagtgttgg tatcgaactt
     4801 ggaatgacac ctcaacttat caaacgtcac cctttccccg gtcccgggtt ggcagtcaga
     4861 attttgggag aagttacccc tcaaaaagtt gccattcttc aggaagctga ctatattttt
     4921 attcaagaat tgcgaaaatg ggacctctat gataaagttt ggcaagcggg atgtgttttg
     4981 ctgcccattc aagcagttgg cgtaatgggt gatgaaagaa cttatgaaaa cgtggtggcg
     5041 ctccgtgctg ttacttcaac agatgccatg actgccgatt gggcaaaatt gccttatgat
     5101 tttttggcca aagtatcaaa cgaaattata aacaaagtaa aaggtataaa ccgggtagtg
     5161 tacgatatca gttctaagcc tccggcaacc attgaatggg aataatttaa aaggtggaat
     5221 tttcaaccaa atcacagtaa agaattcgct gcacaagtag cagctacaaa ttgtattcac
     5281 gatactgtct aaacattgtt gcactttcaa ggtaaaataa aacttttatg tgataattca
     5341 aacaagcatg atagacaccc atgcccatat atattccgaa gaatttgatg ctgaccgaga
     5401 gaaagttata gagagggctc taaaaagagg cattgaacac ataatcttac caaatataga
     5461 tttgcagagc ttacagccca tgttgcaact ggaagcaagc catcctgata tttgtcatgc
     5521 agctattggc cttcaccctg taagcgtgaa agaaaatttt gcagaaaccc tgaatttttt
     5581 aaaattcaaa ttacaacaac acaactatat tgctatagga gaaataggca tagatctgta
     5641 ctgggacaaa acgtatcaaa atgaacaaat tgaagctttt tgtcaacaaa tagaatgggc
     5701 attggaatgg gaattaccag taattattca tgtaagggat tcgtttaagg aaactatgca
     5761 ggctcttgaa ccctacagga ataaaaagtt aaggggtgtt ttccatagtt ttacgggaac
     5821 aatagaagaa gcaaaccaga tcataacttt tggaggtttt aaaattggta tcaatggaat
     5881 actgacattt aaaaattctg aactttctaa aactgtcagc caaattgaga cagagtattt
     5941 ggtattggaa accgatgcac cttatttgtc gcccgtccct tttcgtggaa agagaaatga
     6001 gccggcttac ctatcctata tatgtgagca actcgcgctg atacttggga aaacatcgga
     6061 agaagtcgag tttatcacta cccaaaatgc aaagaaattg tttaaattac agccttaaca
     6121 gtaaaaaaaa ataaaaaaaa caagaatctg attataaaat aaaaacaaat atattgtatt
     6181 tcattttttt ttcttaactt gcaccccaaa atggagaggt gctcgagggg ttgaagaggt
     6241 gcgtctggaa agcgtatata cggtgttgaa ccgtatcgag agttcgaatc tcttcctctc
     6301 cgcaacatca aaacaaaaac aaaaaaaaca agcagtcaaa atttaaaaca aaaaattaga
     6361 aatcttaaaa tttaaagaaa aatgaaaaag gtatttgcaa tcttatcgat cgtagcaatt
     6421 ttttctttcg gaagcgctct gcatgcttcg gctcaagata caagcgctcc tcaaaccgag
     6481 cagacagccg gcaatgatac ggctgtagca gctgcacaag caatggaaca ggtagaagcc
     6541 cctgccccag cacaaggtgg tggcatgcat aaggctttaa aacaaaaatt tatcgaagga
     6601 agcccggcat ggatggcacc tattgcactc tgcttaatta ttggattggc tttgtgtatt
     6661 gaacgtataa tttatttgaa tcttgcttct accaacacca agaaactctt ggaggatatt
     6721 gaccaagctt gggctaaagg aggtgttcaa gctgctatgg aagtttgtag aaataccaga
     6781 ggtccggtag cttcaatttt ttatcaggga ctttcacggt acgatgaagg aattgatgta
     6841 gttgaaaaag ctgttgcttc ttatggtggc gtacaattag gattgctcga aacaaactta
     6901 acttggatta gcttgtttat cgctatttca ccaagtttgg gattcttagg tactgttatc
     6961 ggaatgattg aagcatttga tagaattcag caagtaggcg atatttccgc aacagtggta
     7021 gccgggggta ttaaagttgc tcttttgaca acggtatttg gtcttatcgt tgctatggtg
     7081 ttgcaggtat tctacaatta catcctaacc attatcgaaa acatgaccaa tgatatggaa
     7141 gactcttcaa tttcattgct cgatatcatt gtaaaacatt cgaaaaaata aattcgtaaa
     7201 aacttaataa ggttatgtcg aaattcatgc aaaaaatacc tataatttca gcaattttcc
     7261 tggtatcggt atcggtaatc ttggtattgc tgatttatgt agggggaacc tcaggttcga
     7321 taaacaatag cgccggtgag gcaatgtcag ttccaagatt tactgatgca ctaatttact
     7381 ggtcatattt tcttgtggga ttagctgtag ttattacatt gggctttgta gttaaaaatt
     7441 ttttccaatc ttcaaaacaa agttcatcat ctatttttaa gtctcttctg cctatcatag
     7501 gatttattct tttgttcgtt gtttcatggc tacttggcag tgatcaggaa ttgaaaatta
     7561 tcggatatga aggagcagag aacgttggat tctggtcaag atttaccgac atgatgattt
     7621 ttccgattta tgtaatggca ataggaatta tattaacaat cataggtact tctatttatt
     7681 ccagtctaag aaaagattaa aactgaattt tgagttaata aaagtttgac tcttactaat
     7741 aatatattaa aatgtcgaag aaaaaaagaa aagtaggtca aataaatgcc agttcaatgg
     7801 ctgacatttc atttttactt ttaatctttt tccttgttac tacatccatg aatgttggaa
     7861 cgggtatcgc aagaagattg ccgcctccgc tgcctcccga tcaaaaacca ccagacatgg
     7921 acataaaaaa acggaatatt ttcattgtcc gtatcaacag ccaaaaccaa attatggcac
     7981 aaggagaaat attagatata aaacagttaa ggtataaaac aaaggaattt ataaaaaatg
     8041 agaataatga ccctaatttg ccggaactga ttactgaaaa tattccctta ttgggagaag
     8101 ttacttatac taaaaatcat gtgatttcac ttcagaatga tgtggggacg caataccaag
     8161 cttacataga tgtgcaaaac gaattgattg cagcatataa tgaactgcgc gatgagtttg
     8221 ccatgcaacg ttttgggaaa aaatacaacg agttaagcca agagttgcaa gaagcagttc
     8281 aaaaaatcta tccacaaaaa atttcggaag ctgaacccaa aaattatgga ggagtaaaaa
     8341 gtggcaagaa ttagaaaaag cgaaaaacga gaaatgccgg cattgagtac ttcttcttta
     8401 ccggatatca tcttcatgct gttgtttttc tttatggctg ttacaaccat gaaagaagta
     8461 acctataagg tcaggattga tcctcctaaa gctacggaaa ttcagaaatt ggaaaacaag
     8521 tcgctcattc gatatatcta tgtcggtaag cctttggagc agtatcaaag aatttatggt
     8581 agcgaaaccc gaattcagtt gagtgatatg tttgcagata tttctgaaat agaagagtat
     8641 atcattaacg aacgtagtgc aatgaaggaa gaagatcaga atttaatgag tgtatgcatt
     8701 agagctgaca aagatactca tatgggtatt atcactgata taaagcaagc tttgcgaaga
     8761 gcctacgcac ttaaaattat ctatgctgcc aatcagcgta cacagtatta atcttcttag
     8821 tttttagtgt tatccaaata ggctgtctca taagtgcgac agcctatttt tttgtgcttt
     8881 gcaggatata gaaaaattta ttttacaaat aacaccgatt ggtaaaattc aaccgcattt
     8941 ttacctaaat caaaaacatt attttgaacg ttttaaattg agatgagaat caagatgaga
     9001 attcgtgaaa gtatcattcc tttttcaatc acatttttta aaatatactc aataaacggc
     9061 cggttgcagg gtcaataatt actattttgt cattaccttt gatcatggaa gacgatagcg
     9121 gaattacaat gccaaactgt gggaacaaaa attgttgaga aaaaaatgtg cttttcccat
     9181 cggaaacagt aaccaatgta gcagaaggaa taacaaagtt tatgtattct ttgggtgctt
     9241 tttgtttggg gttgtccaat atggttggag atttagtagg aataacacta atatagtacg
     9301 gaacgccact caaatcatca gctggcacaa gcccttttaa aaccgaaaaa cgaaaaagga
     9361 cttcgttgtt caaagcttgt tgaggtcgta aatataaggt atgtttttga atttctttcg
     9421 ttgtactacc aataaaaagt tctgttaact ctttttcttt gttatccaat tcatttaaaa
     9481 tggcactcaa tgatgttcca tcagaaggga aatgatcaac gtctccggta agtaagttta
     9541 atctgctttc ccgaattcta tatatctgtt tggcagctcc ttctgcaagt tttgctactg
     9601 acccaaccaa catatattct tcacccagcg gcaatataac gttattgcta agattttctt
     9661 ttgcttctgt gggtgcaaac actgaaagtt cgggcaaaga ggtgggagga ggaacattta
     9721 ttccacataa taagccgttt tgatcaaccg agatggacac aaagggcgaa tttttatcca
     9781 ctctaacggc ataggttttc gatggatcgg gaacggcatt tgaagttacc tgaatatctt
     9841 ttaaagaata aactgttttt tcttcggtaa ttatatctga agtagccaaa tatctttcag
     9901 aataacgata aaaaacaccg ggttttcggg taattttttc cacgtcaact ttaatacata
     9961 attctgtcat gggtaaagag taaataagca ctgactcgtt ttctttcaat gtgagggaag
    10021 attgacataa cacgctcgaa ataacagaag ccataaggaa tgaaacaatt atttttttca
    10081 taaaactgaa tttggttgat tgatgttgtt aataaagcag gaaatataaa atattcagaa
    10141 agatttttcg actttttgaa aaaatttacc ttaaaggatg cttctttata tcatactata
    10201 aaatcaactt tattgaaatt cattgatttc tgagcaaata tagaaaaaca ggtgcaaatt
    10261 acaaaaaaga aaatctaaaa aatatttcag ttggtaagaa gaaagtataa atactttaga
    10321 aacatgttgt tgttaccgat ttataaaaaa tcttatttct gttctacaac gcagaacgtt
    10381 tgtaaaagta taaatttatt tttattatct ttgtccagct taaaaaaaat catttcttgg
    10441 ctgcaaaaaa ggcaatatgc cttacttata tggaagaatt ttaatgtcga tttcataaaa
    10501 aagaataaat tgccttatta attttatggg taaaattata tcgttggcca atcagaaggg
    10561 cggagttggt aaaaccacta ctgctattaa tttggctgct tctttggcat cattgggtaa
    10621 aaaagttttg ctggttgatg ccgatcctca ggcaaatgcc tcttcgggat tgggaataga
    10681 aattcaatcg ctgaaatata ccatttacga atgtttgatt gatggtgtgt cgccacagca
    10741 agctattcat ggaacggaaa tagaaaattt gtcggtgctc ccttcacata tcgatttggt
    10801 tggagcagag attgaaatgt tgaacatgga ggagcgggaa aaagtgatgc agcatttgct
    10861 gcgcccgtta aaacccgatt tcgattttat tttgattgat tgctcccctt cattgggttt
    10921 gataacggta aatgcactta cggcttccga ctcggtgatt attcccgtgc aatgtgaata
    10981 ttttgcattg gaaggtatca gcaaactttt aaataccatc aaaataatta aaagcaagtt
    11041 gaatccttcg cttgatattg aaggcttttt gcttaccatg tacgacaatc gtcttcggtt
    11101 ggcaaaccag gtttatgccg aagtaaaaaa acattttcag aacatggttt ttgaaaatgt
    11161 catatacaga aatgtgcggc tcagcgaagc gccaagttac gggaaacccg ttatgctgta
    11221 cgatcccgaa tcgaaaggtt cttccaatta cctcgatttg gcaaaggaat tgattgaccg
    11281 acaaaaaagt taatatagct tgcaataaaa aataaaaaga ataaaatctc gtttgcagtt
    11341 atggtaaaaa aatcagcatt gggaagagga cttggagcgt tgatcgacac cgacgacatt
    11401 tataccagtg gttcttcttc cattagcgaa gtggatgtgg atttgatttt tccaaatccc
    11461 aatcagccac gttctcactt tgatgaggaa tctttggccg aactggcttc ttccatacgt
    11521 gaactggggg ttatttcacc cattacgctt cggaaaaatg atgatggaac gtatctgatc
    11581 attgcgggtg aacggcgtta tcgtgcttcc aagatggtgg ggctcaaatc cataccggcg
    11641 tatgttaaaa cggcagccga cgagcaagtg gtggaaatgg cactgataga aaacattcag
    11701 cgcgaagatt tgaatgccat tgaagtggct ttggcttttt accgactgtt ggaagaatat
    11761 catcttacgc aggagcagct tagcgaacga gttggcaaga aaagggctac cattgccaat
    11821 tacttgcgtt tacttcgttt gccggccgaa atccaggtgg gaattaagga taaaaaaatc
    11881 gacatgggac acgcccgtgc tatcttggga ttggaagatc ctgctgtaca acttcaactc
    11941 tacgaagcta ttctggagaa tttttattct gtcagaaaag tggaagaaat ggtcagactc
    12001 tacgccgaaa ccggaagttt tgaggaaaaa catgctcgag tttccaaacc caatccggta
    12061 agaaaattaa aagaatacga agaattaaag aatcaactga gtaaaacttt cggcactaaa
    12121 gtacaattta gttgtaacga gcagggaaag ggaaaaattt ccatcccatt ttcaaacgat
    12181 gaagaattat cccgtatcat gggattgctg gataaaattt aaaccaaatt tcaagtgaaa
    12241 agattttttc tgataacggc agaaatatgg tttatctccc ttttcggaat tgtactcaat
    12301 gctcagaatg tcaacgaaaa aagagagatt gttcaggata gtataaatcg gctgcctgaa
    12361 aaatctttac aaaaagaaag ccaagtatca attgatactg tacaaaagcc ggtttacatg
    12421 cccaatccgt cgaaaattgt ttggatgggt gcagtagtac caggttgggg acaaatattg
    12481 aacaggaaat actggaaatt gccaattgtg tatggcggat tgttgggatg ttattatgcc
    12541 tattcatgga atgcaagccg gtataattct tatcataccg cttatttgga tattattgat
    12601 tcagacccga cgacaaacag ttttctggat attttgccgg caggatatac tcttgaatca
    12661 attggcggga ccggcagata tacaaccatt ttaaaaagcg gacaagatca atttcgtcgg
    12721 cagagagatt tgagtgtaat tgtttcggtg gctttttatg ctcttacgtt ggttgatgct
    12781 tttgtggatg cacaattgta cggcttcgac attagtcccg atttgtcgct tgaaatcaaa
    12841 ccaatttatc taaaagataa tataactaca tcaggggggc ttggcatgca atgccgtttt
    12901 aagttgaaat aattaccccg cattattagg ataaaaaatg aatttaaaat tttccataat
    12961 tgttgtttct ttcatttgct tttcgataaa ttcagttatt tcacaagctg ccaaagattt
    13021 accggaagat actttatctt ccaaaatcga cagcatcatt ggaatacctg acgcatacgg
    13081 atatgcatta gatcatttgc ttcatctatg ggcattgcag cgaatggata ctacagaatg
    13141 caattcgggt gagccacccg caccggttcc cgattctcta tataaatatc ggctctcaca
    13201 aataccttgt ttgatagagc ttccttataa tagtattgtt cgttccttta tagaacttta
    13261 tactgtccga aagcgaactc aaacagaata catgctgggc atcagcaatt attatttccc
    13321 tgttttcgaa caagcattag atgccaataa attgccttta gaattaaaat ttctccctat
    13381 cattgaatct gctttgcacc ctactgctat ttcacgagct ggagctgccg gtttgtggca
    13441 atttatgatt ccaacagggc gaatgtatgg tttggaaatc aacacattgg ttgacgaacg
    13501 gcttgatcct cgaaaagcgt cctatgctgc agcgcggttt cttaaagatt tatatgccat
    13561 ttacggagac tggaatttgg tgattgctgc ttacaattgc ggtcccggta atgtgaacaa
    13621 agccatcaga agagccggcg gaaaaaccga ttattgggca atttaccctt atttgccatt
    13681 ggaaacacga ggctatgttc ccatttatat tgctgccacc tatacttttc attatgccga
    13741 agaacataat ttatgtccag cagtggtaaa tattccagca ctaattgaca ctttcatgat
    13801 caatcaaaga atccatttgg aacaaatatc gtcagtgctt aatatcccga ttgaagagct
    13861 ccggtggctt aatccacaat ataaaaagga cataataccc ggtgatttaa aaccctatgt
    13921 cttaactttg cctgtaaatt attccagcat ttttatggag agattgcatg aaatagcttc
    13981 ttataaagct gatgaattaa tcaataaccg tagaaacgaa attgaagctt tcaggtcaac
    14041 ttcttcttct aatttagggg cttccaaaaa tgttacttat cacaaagtaa aaaaaggtga
    14101 gaccttgagc ggcattgctg caaaatatgg tgtttcagtc tccaacctta aaaaatggaa
    14161 taacatcaag ggtagtaaaa taacaattgg ccaacgtctg aaaattatca aataaaattc
    14221 aacatggaaa aagtatatta ttccatttcg gaagtagcag aaatgctgaa aatcagtcct
    14281 tccaacctgc ggttttggga aaaggaattt tcgcagctga agccaaaacg aagcaacaag
    14341 ggaacacgta cttataccaa agaagatatt gaaattatta aaatgattca cttactggtc
    14401 aatgagcaga aactcacatt gcaaggcgca aagcgtaaac tttcgctgaa aaaggagacc
    14461 gtttccaaac agcaggagtt ggtggaacgt ttacaaaatg tgagaaggga attgttgatg
    14521 atcagtaagg ctttggatga ataggcaatt tacaaataga atataaaatg aaaaaaacga
    14581 atttttgttt aggcttgata gctgcattaa ttttgatgtc agcttcggtt tctgctgaga
    14641 aaaagccaaa aaacgtaatt ctgatgattg gtgatggtat gggtttagcc caaatgtatt
    14701 cggcaatgac tgccaacaac aatcaacttc agctcgaacg gtgcacttac accggttttt
    14761 caaaaactta ttcttccgat cattatacta ccgattcagg tgccggagga acagccatag
    14821 cttgtggcgt aaaaacaaaa aacggcatga ttggcatgac gcccgattcc gtaaaaatag
    14881 aatctattct cgaactctcc gaacgcaatc agttgtccac cggaatagta gtaacttgtg
    14941 cagtaaccca tgcaacaccc gccagcttta tcgcacatca gattagccgc aatatgtatg
    15001 aagaaatagc tggagatttt cttcaaacag atgttgatgt gttcattggt ggcggactga
    15061 agtattttaa taacagaaaa gaccagataa acttagtaga aaaactaaaa aaaaataatt
    15121 atcaaatagc atattcactc gacgaagctg taaaagtaaa atcgggaaaa ttggccggat
    15181 tgttttatga gaatcattgc ccgccgatgc ctgagcgtgg cgatatgctc ccaaaatcgg
    15241 taatggcagc catagatatt ttggacaaca acaagaaagg ttttttcctg atggtggaag
    15301 gttctcaaat cgactgggct gctcacgaca acgacaagaa acagatcgta aaagagatgt
    15361 tggattttga tgaaaccatt ggaaaggttt tggattatgc ccagaaagat ggcaatacat
    15421 tggtaattat tacagccgat catgaaaccg gcggaatgac tattttaaat ggaaaattcg
    15481 gatctgacga aatggatgcc catttttcta ccaaaaacca ctcgggtgtt cctgtgccga
    15541 tctatgcttt tggacccgga gccgaacagt ttaccggtat tatggaaaat acagcgttta
    15601 aagggaaaat agaaaagtta ctcgatttaa aaaaataatt tttcattatt acttgttgaa
    15661 cagttaaatt tttaacagtt cggtcgtaaa tttttctgat atattcattt tcaatttaat
    15721 aaaaagacaa tataatgata acaatcgaac aacttaaaga cgttacagaa cgcaccgaag
    15781 cgttgaggag gtatctttga cgtcgacaac aggcaaattc aaatagaaga agaagaactt
    15841 cgtacgcagg aacccaactt ttgggacgac ccgaaaaagg cagaagctca aatgcgaaaa
    15901 ttaaaagagc ttaaaaattg ggtaaccggt taccaggaag tgaaaaccgc agcagatgag
    15961 ctggagcttg cttatgattt ttttcgtgaa ggcgtgattg aagaacagga attggacgaa
    16021 acctatcgga aggctttaca attgattgaa cagttggaaa tgaacaacat gctttctcat
    16081 gaggaagatc agttgggggc tgttcttaaa attgttgccg gagccggcgg aaccgaagct
    16141 caggattggg cggaaatgct gatgagaatg tatcagcgat gggcagaaag aaaaggttat
    16201 aaatgggaga ttaccaactt ccagcccggc gatgaggcag gaattaaatc cgtaactatt
    16261 cagatagaag gagaaagagc ttacggctat ctgaaaagtg aaaatggcgt tcaccgttta
    16321 gtcagagtct ctcctttcaa tgctcaggga aaaagaatga cttcttttac ctcggttttt
    16381 gttgttccgc tgattgatga ttccatcgaa atagagatta atccggccaa tctttcgtgg
    16441 gatacttttc gctcgtcggg agctggcgga caaaatgtga ataaggttga aaccggtgta
    16501 agattgcatt acacctttaa acatcccgta acaggggaac agagcgaaat cgttattgaa
    16561 aacaccgaaa gtcgttcaca attcgataac aaggacaatg ctctgcgttt actcaaatcg
    16621 caactttacg agcttgaact tcagaaacga cgggctgaac aggagaaagt tgaatcgacc
    16681 aaaaagaaaa ttgaatgggg ttcgcagatt cgcagttatg tttttgacga ccgtagggtg
    16741 aaagatcatc gaaccaatta tcaaacctcc aatgtgcagg cagtgatgga tggtgatatt
    16801 gatgaattta tcaaagctta tttgatggaa tttcccgact aagagaaaaa cgttaattgc
    16861 tttttcccaa attccatatt ttgatcgaaa atgtttgtct tcaaaaaaaa tggagtaggg
    16921 gatatctctt ttttctattt aaacccagat tactcattaa ccaatttaaa gggaaagtca
    16981 aagtcagaag agttgttcta tagtcaggaa aactatgctc gcctttttta tttatttaaa
    17041 tggatttgat aactggtttg ctgttgattt tttcatgata tgcattcgac tgcagaagtt
    17101 ctatatcttt agataaagtt attttataca aaattaaaac cctttttcta ttgaggattc
    17161 tgagttaaaa taagacatcg gacaaaaaaa ttgcaacttt caatattcaa ttttttttgc
    17221 gaaatttgca aacttaaaga atttaagtaa aaacggcaaa gtgaaagaaa ctaaatacat
    17281 ttttgttaca ggaggtgtag tttcctctct gggaaaagga attattgcag cttcgttggg
    17341 gaaactcctg cagtcgagag gttttaaggt gaccaaccaa aaacttgatc cttacataaa
    17401 tattgatccg gggacattga atccttatga acacggggaa tgttatgtga ccgttgatgg
    17461 acatgaagcc gaccttgatc tggggcatta tgagagattt ttgaacattg agacccatcg
    17521 tgcaaataat attaccaccg ggcgaattta tcaaaatgtt attgaaaaag aacgtcgtgg
    17581 agattatttg ggaaaaaccg tacagataat tcctcacatt acggatgaaa taaagcgaaa
    17641 tattaaatta ttgggctcaa aaggcgatta cgattttgta atcacagaaa ttggcggtac
    17701 tgttggcgat attgaatcat tgccctatat tgaaagtgtc cgtcagttgc gatgggaact
    17761 cgggaaaaat tgtttgattg ttcatttgac ttatgtaccc tatatttcag ctgctaagga
    17821 actaaaaaca aaacctactc aacattcagt caaagcttta caggaacaag gtgtacaacc
    17881 cgatatcctg gtattgcgta ccgagcacaa tatttcttcc gatttgcgta agaaagtggc
    17941 tttgttttgt aatgtcgaac ccaattcggt tatgcagtct gtagatgttt ctaccatata
    18001 tcgtgtccca ttaatgatgt acgatgaaaa acttgatgaa gtagtactta gaaaaatggg
    18061 gtttgacttg ttgaaaatac ctcaggccaa tctttccaaa tggatacagt ttgtggaaaa
    18121 attggaaaat gcaaaagaag aagtacacat tgcattggtg ggcaaatatg ttcagcttcc
    18181 tgatgcttat aaatctatca tcgaatctct acttcaggca tgtgcttata acgacagaaa
    18241 attaaagctc gatttaatat tgtcggataa acttaccgaa gaaaatatag aaaaaaaact
    18301 tgagggagtc gatggtgtta ttatcgctcc cggttttggt caaagaggaa ttgagggaaa
    18361 attagtcacc atcaagtaca cacgtgaaaa aaacatacca actttcggca tttgtatggg
    18421 gatgcagtgt atggtgatag aatttgcaag aaatgtgctg ggttatgcag atgccaacag
    18481 caccgaaatt gacccgacta ccactcataa tgtagtggac attatggaag aacagaaaaa
    18541 tattttgaat ttgggtggca gcatgcgatt gggagcattt ccctgcaaat tgaaaaaggg
    18601 gacaaaagtt tttaagattt acggaaaaga aaatatcagc gaacgacatc gacaccggtt
    18661 tgaatttaat aactattata aagaagagtt tgataaagcc ggcatgattt gcagtggaat
    18721 aaatgcttct tcaaatttgg tagaaattgt tgaagttcct tctcatagat ggtttatcgg
    18781 cgttcagttt catcccgaat acagcagtac ggttattaat cctcatcctc tttttatcag
    18841 ttttataaaa gctgccatcg aatatagaaa cgaatcgaag gaaaaaactc aaaactcaaa
    18901 atttaaaaca gctattaaat aattaatgat tgaaaattta attctcattt ctcacttgtt
    18961 gaatttcaaa aatatatcat ggataaaaat acaattacag gttttctatt aataacggtt
    19021 attattgttg tctttgcctt attgaatcgc cccaatcagg aaatgatcga aaaacaaagg
    19081 cgctacaacg attcgatagc acaaattgaa aaagccaaag ctgaatcgga aactgctatt
    19141 caagaagtca attctttact ttctgattct attaaaacca cactggattc ttcggctgtt
    19201 atcagagatg cttatggcga tttcagtgtt gccgctttag ggaaagaaga atttataaca
    19261 ttggaaaaca gcgttttaaa acttaccttt acgaacaagg gggctcgtat ttatgcagcc
    19321 gaactaaaag attatcgtcg acatgattcc cttcctctga ttctttttca ggggagtgag
    19381 gccaatatgg gatttactct gataaccaac aataacagaa ttctgaacac agccaactta
    19441 ttttttgaag ttttacttcc catagaaaaa gatacattgg gaaatcaaat acttgttttc
    19501 agactaaaaa ctaccggcga aggatacatg gatgtggtct acactcttcc tcccgacaat
    19561 tatatgttta gtttcagcat taaagccaac aaaatgagca atgttatgcc ggcgggaact
    19621 aattttttgg aaatgcagtg gactacccga ttgcgtcagc aggaaaaagg tcggcaattt
    19681 gagagtcgtt actcaaatat tcagtataaa tatctttcgg atgatgtaga aaaattaagt
    19741 gaaagtaaaa atgacagcaa gcaaattacc aacaaactga aatggattgc tttcaaggac
    19801 cagtttttca gtaatgtgct tatagccgaa gatgcattta cttcgtcttt tctggaaagc
    19861 aaggttgaac ctgaggattc tccctatctg aagtcgtaca gggccgatat gaccattcca
    19921 tttgacccta ccggaaaaac ttcgaccaac tttagaatgt atttgggtcc taataaattc
    19981 aatatccttg catcttatga caaaggagta gccaaagaag aaaagcttca acttcataaa
    20041 ctgataccac ttggatgggg aatttttgga tggattaacc gttacatgat tattcccatg
    20101 tttaatttct tcagtaaata catcgggaat tatggcatca tcatcttatt gatgacaatt
    20161 atcattaaaa tcataatttt tcccatgacg tataaatcat atacatccag cgccaaaatg
    20221 cgagtcctta aaccagaaat tgatgaaatt tacaacaaaa tacctcctga aaaagctatg
    20281 gaaaggcaaa aagcagtcat ggatctttat tccaaagttg gtgtaagtcc catgagtgga
    20341 tgtcttccgg ctttgttgca aatacctatc ctttttgcca tgtttagctt cttccctgct
    20401 tcgatcgaac tccgtcagga aagttttctt tgggcaaaag atctttcaac ttacgattct
    20461 ataattagct ggaatgctta tatcccaatt atttcgaagt attttggcaa tcatttaagc
    20521 cttttctgcc ttttgatgac catttcaagc attatttcca ccaagttgac aatgacaact
    20581 actccaacta ccaaccaacc gggcggaaat ttaatgaagt ggatgatgta cttgatgcct
    20641 gtcatgttta tgtttatttt taacaattac tcggcagcat taagttacta ttacttgctt
    20701 tctactctga ttaccattat tcaaactttt atcattcgct caatggttga cgaaaagaaa
    20761 cttttagccc aactacatgc taaacgcaat cagaagcaac ctgcgaagaa aaaaagcagt
    20821 tggattgaac gaatggaaaa aatgcaacgc gaacaacaaa aagcattgaa aaacaaaaga
    20881 taagagtttt agaaaataat atgtttaagc gtaaaattgc ataaaaaatt ttgtggtttt
    20941 acgctttctt ttaaaattca atcatgacaa aaaaaatcaa gttttttatt ctgttttttc
    21001 tgattatatt tcttttctcg tgtcaaaatc ataaccgttt tcgcattaac acaaaaaaga
    21061 atgaagttga agttcggata cggcgttttg ataaggattt gatcaacgtt gacactacaa
    21121 atctcaaagc agaaattagg aaaatttaca aagattatcc cgtttttttc ccggtattta
    21181 ttaacgaaat acttaacgtt gcaccttccg acaccgattc agttgctaaa ttaataggag
    21241 attttctttg tgatgccgct tttcaaaaag tcaacaaaga agttctgaaa acttttgctg
    21301 atgtgcatga tatagagaaa caactttcga tagcttttac ttatatgcat cattattttc
    21361 ccgagatcag gctacccgaa atttattttt tcgtatccgg cttcaatcgt tctatcatta
    21421 tttacccttc tttaattgct gtaggggccg atttatactt aggagccgat taccctcttt
    21481 ataagtcgtt tacttatgaa tacctgactt tcaatatgcg acctgaaagt atagcccctg
    21541 atgttgtttc tacgcttctt tttagttatt tcccaatgga atcggaaaat aatcgcttgt
    21601 tggacaatat gctgtataat gggaaaattt tatttttgtt gtcggtcttt atgcctgatg
    21661 tagaaccttc agtattgatg gggtatactc gcgaacaatg gcaatggtgt aaacgatttg
    21721 aaaaggaaat atgggcaaaa attattgaag acaaagcatt gttttcttcc gatatgaggc
    21781 ttataaattc ctatttggat gaagctcctt ttactgctcc tgtatcacaa gattctccag
    21841 gtagagtggg cacatggata ggttggcaaa ttgtgaatag ttatatgaaa aaaaacaata
    21901 acgtcagtct acgtcagtta gctaatgaaa ataattatca gctactgctc cagaaatcgg
    21961 actaccgtcc ataaaaaaag cggttccgtc atgctttaat gacagaaccg caaacaaaat
    22021 aaattaaatc gaaaatttaa ttatgcaggt atgattgctt ctgatggaca aacatcagcg
    22081 catacaccgc aatctgtaca cacatccgga tcaataacat agatatcgcc ttctgagatt
    22141 gcttctaccg gacattcagt tatgcacgat ccacatgcaa tgcaatcttc tgtaattacg
    22201 taagccattt ttatatagat ttagtttaat gctgcaaagt tacttttttt tctcaaacca
    22261 aaaaaatttc acaacactta ttcacttttg aaatttctaa agtcgtaatt tttttatcag
    22321 gtgagagaca gattattttg ttatgctcac aaacaaattt ttcctttcat cagaatataa
    22381 tttatttttt tagagtctga tggaacccac atgtctgaat tattatattc attcgtgttt
    22441 gtgtatgcaa tacatgtggg tttcatgatg aattttaaaa tctcattaag cctttaattc
    22501 tgagatggga tattttatct tacatttcta aagtaaattg ttgaaacata tcttaaacat
    22561 tggtattaca taccaaagtc aattatcatt gtttggaatt tatcacatcc agcgctttca
    22621 atacagtttt gtcgcttcga taaagtaatg gataaaatcc ttcatcgccc agaatatttc
    22681 gcgaaatata ccctttaatt tgtgtcaaaa gcaaattttt tgagatgttt atttcttttt
    22741 tattggcttg aattcctttt tcggaaacaa attttacaaa ttgatccaat aaggattgac
    22801 tatttaagta agtgtccatt tcgtgccaat tctttatttt tttcagtttc tctctgtttt
    22861 tgtcagaata ttgaaaggca aactgataaa catatccgta attgaccacg ttattaaaat
    22921 atggtgtaaa ttctgatgta tccaaaggaa catatacatc gggcataatg ccgcctccgc
    22981 catatacaat tcttcctcca accgttttgt atcgcaaaga atccgggtgg aaaatactgt
    23041 cgttaatttc atgcaacaga cggttgataa attctttttc gtattcttcc gaattaccaa
    23101 gtttataagg cttttgaata cttcgaccgg aaggggtata atagcgtgcc accgttaggc
    23161 gaacagccga accatcagac aaattaaact gctgttgcac caatccctta ccaaaagtac
    23221 gtctgcctac taacgttccg cgatcgttgt cttgaatggc ccctgcaaaa atttcacttg
    23281 ccgatgcaga aaattcatca acgagtacta ccagcggctc ccttgtacag ctaccctttc
    23341 catctgattt ggcttcaaag cgcttatagg ctttcccttg tgcataaacg ataagtcggt
    23401 tagccggcag aaattcgtta atcatattaa tggactgatc cataaatccg ccactattgc
    23461 ctcgcaagtc aattatgaat ttatcagcac cctcgttttt taatttggct atggcattca
    23521 gaaattctcc ataagttgtt tcggcaaatt tgctgacttt aataaaaccc acacttggct
    23581 gaacaatata agcaatatct acggaattta caggaatttt tcctctggtt atttcataat
    23641 acaacacttc ctttgtttgg tttcgtttaa tgcccaactt aactttcgta ccttccggtc
    23701 cgcgaagttt tttcattact ttttcattgt taatactttt tcccacaaag gtggtgtcat
    23761 ttacttctac aatgcgatct ccgggcatta agccagctgt ttccgaagga cctccgctga
    23821 tcacggatat aatcatgact gtatcgttct gaatgttgaa ttgtacgcca ataccactaa
    23881 aactaccttc caattcgcta tttgccaaag aaatatcttc tgccggtatg tataccgaat
    23941 ggggatcaag ttttgcgatt atatcttgca tgacgttttc agtcatttca tctaagttga
    24001 gtgtatcgac ataattagta ttaataatct gaagcaattc atctactttg gaagcattac
    24061 caaaaccgaa aaaaatgttt ggtgaagacg agaaaaaagc cctctgagcc agtaagttac
    24121 ccagaataag tccaaaaatt acggctaaga atacgatgag gggtgcaaga taattttttt
    24181 tgttgcgatc cataaataaa aattattttt tatgttttcc aaaatattta tttaaatgac
    24241 attgaaatga tggtaaattt catctcttat ttatgaaggt ttttgttctc gccggtaatt
    24301 ttcattttta tcaaacgaac taattctgct tgaaaaaaat gattatctca taaataagtg
    24361 gataaaatga tggaaaaatg accgaaaaaa tttacttaaa agatgaatac aaaattagag
    24421 aattaacttt gtttttctgt aaacatttta ttaaaaataa cgattttgac cgacttttgt
    24481 tctttgaact gtttttcttt gattttatac aatggtttcc tatatccgtt ggtctacttt
    24541 ctctgtaaaa aaaattattg gcaaaaacat tttcattatt ttaaataaaa acaccttata
    24601 tttccacgtt aattattaaa tttgcgaact taattataaa accagaaaaa atgaaagtac
    24661 ctgaaaattt gaaatacacc accgaacacg aatggatcag ggtgcaaggt aatactgcat
    24721 gggtgggaat taccgatttt gctcagcatg aattgggcga aatagtattt gttgaggtag
    24781 aaaccttggg agaaaacatt catgccggcg aagtttttgg aacagtagaa gctgtcaaaa
    24841 cagtttcgga actttttttg ccaataagtg gtgaaattct tgaatttaat aaagaactcg
    24901 aaaacaagcc ggaactgctc aacgatgatc cgtatggcga aggctggatt gttaaaataa
    24961 gggtcgccga tccttctgaa ctggaaaaat tactttcggc agaacaatat gtcaagttga
    25021 ttgcttgaac aagaagtttt ataaatcaat tttttaaaaa tgaaagcaag agcaagtatt
    25081 atcatgggta gcacctccga tctgaaaata atggaaaagg ctgccaagct tttggatgaa
    25141 tttgaaattc cttttgaaat acatgcgtta tcggctcatc gtactccaaa ggaggtagaa
    25201 gattttgctc ttcatgctga aaaaaggggt attgaaataa ttattgccgg ggcaggtatg
    25261 gcagcacatt taccgggtgt tatagcttcg atgactactt tgcctgtgat tggtgttcca
    25321 attgaagctt ctttaaacgg tatggatgcc ttgttttcca tcgttcaaat gcctccgggt
    25381 atacctgttg ctactgtagg gatcaatgct gcacaaaatg ctgcaatttt agctgttcaa
    25441 atattggctt taaaagacga aaaaatcaat tccaaattaa agctgtacaa agaaaatatg
    25501 aaacaaaaaa ttgttgaagc aaacaatcaa ctcaaagaaa taaaatatac ttataaaaca
    25561 aattaattag ctccgtaaac tatcactgag aaagctgaaa ttatcattaa agtttttatt
    25621 cctttttgtt tcattttctg cgaagtatta tcactatttc acgaagtgtg atgtaaatta
    25681 aaaagttgta taaaaaatta aaatcttatt ctttcgacaa ggataagaaa attgattaag
    25741 aataattttc cacttttgaa taggttttgt tcatttttta gcatctaccc tgagggtaaa
    25801 gaaaaccatt ttataacccc gtcgcccgaa gaaaacgaca ttttattttt ggttttttcc
    25861 cctatttccc ctttttcccg ataaccaaca ctgtatcttt ttgtttccag ctttgttgta
    25921 atattgccca aatttcgtac ctttgttcta tggctaaatg ccttcatcat ggcaattttt
    25981 taagaggaag agaccaaagt aatgtatttg tttgatcgat aaccaatgag aaaaattctt
    26041 ctatcatttt caacttgaat gttgcttgaa aaataattct cttttgcttt tatttcccat
    26101 gaaaaaattg catgaattac ttgaatttac gtttgtttta taaagagtgt ttttaaaata
    26161 tgactaccta tccttctcag tctgttttta gtttataccg tcgcnnnnnn nnnnnnnnnn
    26221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26281 nnnnnnnnnn nnnnnnnnnn nnnnttttta aaatatgact acctatcctt ctcagtctgt
    26341 ttttagttta taccgtcgca tggtaacttc tgaaaagaat atgttttctt ttcaggaaag
    26401 gaagaccatc aaacaaattc tgaaggaata ctatctactc catccgcagc aattgacatt
    26461 accgggccat tctatcgaag acaaattgta tattattcag attctgctca atgaaatagg
    26521 attgggaaaa gcttctgttc tgagtgtgct ttttatggaa ccgatcaaga acaattattt
    26581 gactgtttca gaaatagaac aaaaattcaa aactccgctt gactcgataa taggcggaat
    26641 gcagcgtgta gaagagcttt acgaacgcag tttttcgctg gagacagaga atttcagaaa
    26701 acttttattg acttttgctc aagatgttcg tgtcatttta atgatgatag ccgagcgact
    26761 ttattttatg cgaatactgg ataaatttcc tgcagatatg cagcaaagca tggctcgcga
    26821 atgctcgttt ctttatgctc cgctggctca tcgtatggga ctttattcca ttaaaagcga
    26881 aatggaagat ttgtcgctga aatatacaaa tcgggaaatt tatcgtgaaa ttgcccaaaa
    26941 attgaatgaa accaaacgtt ctcgcgacaa gtacattgaa gaatttatta cccctttgaa
    27001 ggaaaaactt ggcaaggaaa ttaaatatcc cttcgaaatc aaaggtcgga cgaaatcaat
    27061 ttattccatt tattccaaaa ttctgaaaca gaatacgcct tttgaaaaca tctacgatct
    27121 ttttgcaatt cggattattc tggatgtacc gttggaaaaa gaaaaaatga tttgctggca
    27181 ggtttattcc attgtaaccg acatgtatca gcccaatccg aaacgtttgc gcgactggct
    27241 ttccataccg aaatcaaatg gttacgaaag ccttcatatc actgttttgg ggcctcaaaa
    27301 caaatgggtg gaaattcaga ttcgcactac gcgtatggac gaaatagccg aaaaaggatt
    27361 ggcagctcat tggaaataca agggaataaa aggtgaaagc ggcatggacg aatggctgaa
    27421 aaatattcgc gatattttgg aaaatcccga cttgaatgcc gtagatttta tggatgaatt
    27481 caagttgaat ctttacgatc aggaagtttt cgtttttact ccggcgggag atttgcacaa
    27541 gttgccaaaa ggcgctacgg tactggattt cgcttatctg attcacagca atttgggaag
    27601 taagtgtatt ggagccaaaa tcaacaagga aagaaatgtg ccgataaaat atgtgctgaa
    27661 aaacggagat caggtggaga tcctcacatc tccctcccaa aagccaaatc cttcgtggct
    27721 gaacatagtg gtaacttcaa aggcgaaatc gaaaattcgt caggcgctga aagatataga
    27781 gtttaaagat gccgaaattg gtcgggaaat tcttttgcgg cgtttcaaaa actggaaaat
    27841 tgagttggaa gaaggaaagc tttcgcgatt ggccaagaaa aaaggttata aaaccgtcag
    27901 cgatttttat caggccattg cacatgaaaa aatcgacatg ctgctcattc gcgatgccta
    27961 tctcgacctc gaccggcatg aaacgggagc agaacaggct gaacctcgga gtgccgaaac
    28021 tttttcaaaa gaaactcctg ccgaagaact ttcttcaaaa gaagatgttc tgattatcgg
    28081 ccaaaatctg aaaaatgtgg attacacact tgccaaatgt tgtcaaccca tttacggaga
    28141 cgacattttt gggtttgtat ccgttggcgg aggcatcaaa attcatcgaa ccacttgccc
    28201 caatgctcct cagttgattt caaaatacgg ttatcgaatt atcaaggctc aatgggccgg
    28261 acgctcagga ggaactcagt atccgattac actttatatt gtcgggaaag atgacatcgg
    28321 tattgtaacc aacattactt cacttatttc gaaagagaaa aatattttgt tgcgttccat
    28381 atcggttgat tccaatgccg gtgtttttca gggaaacctt acaataatgg taaacgatgc
    28441 caatgatttg gacagtatca taaaaaaaat aaaacaggta aaaggtgttc tgaatatttc
    28501 aagaaccaaa agtttaaccc aaaacaacca gtaaaatttt ataaacaatg ttttcaggaa
    28561 taattgaaga agctgccccg gtagtagctt taaaaaaaga agccggcaat ttgcacatta
    28621 ccatgacttg ctcgttcgta aacgaattgt cggtcgatca gagtgtttcg cacaatggcg
    28681 tatgcctcac cgttgtttcc aaaactcccg atacctacac cgtaacggct atccgtgaaa
    28741 cgttggaaaa aagcaatttg ggattgctcc aagaagggag taaggtgaat ctggaaagga
    28801 gtttgctact caccgaacgt ctcgatggac atattgttca gggacatgtc gatacaacag
    28861 cagtttgtac cgatatttct gaagctgatg gcagctggta ttttactttt caatatgatg
    28921 tggacaggga attggcaaaa agaggttatg ttaccgttga caaaggttca gtggcggtga
    28981 atggagtcag tcttaccgtg tgcaacccga cggaaaatac ctttcaggtt gcgataatcc
    29041 cctatactta cgaacacacc aattttcatc agattgtcgt ggggacgaca gtcaatattg
    29101 agtttgatat tattggaaaa tatatcagta ggttgatgaa attgtaaata attccctgat
    29161 tttttttgtt ttatcaaact aaaatcgatg gcaattgtta tataacattg caaaatccat
    29221 ttagaatttg gtaatggaaa atttcaggaa acagataaaa atttcatttt tcaatcccat
    29281 tattcatttt cttccgcttc tgctgttttt ggtaacagct atgtatgccg gaagaacggt
    29341 ggcgaattat gtttccattt tcgtccttct tgtgtcggcg gtatatgttt ttattgctta
    29401 taatcagata tttatttggt ttttcctgtc gtgtgtcatc tttttggcgg tcaatattgt
    29461 tggcagtata attcgtttgc cgataatttt tctgagtttt cgacctttgt tggatgaagc
    29521 gttgacattg gtatttttgg gaggaatgat attgctgcaa aaacccataa aaaaaggaat
    29581 tatggcactc attcccaaaa ctttgccaat gaataacaac cttgatgaat atttcggttt
    29641 tatccgaact attttcttga taattttggg atatgttttg gctaccggtt ttttctatgc
    29701 ttttcctacg cagcgggaaa cggcttattt gcaactggtg caggcaattt atgtcggaat
    29761 tcttttcttt gttttggttt tcgaatcgct tcgggttcaa attattcgtg cacgcttgct
    29821 gaaagaagaa tggttgccga tagtcaattt gcagggaaaa attattggca gcgtacaaca
    29881 tcaagccagt ttgggcgaaa aacgaaaatt catgcatccc attattcgtg tacatattat
    29941 tcaggatggc atgattttgc tcagaaagcg tcctgctgac gatttgtcct atcctttaca
    30001 ttgggatacg gctatttcaa atcatatccg catgggagaa acagttgaag actgcattaa
    30061 aaggacggct caagagctat atggtttgac cgagttaaaa tcttttttcc tttccaatta
    30121 tattacccac acagattttg aacagcatta tgcttttctt tttattgcct gcaaaatgaa
    30181 taaaatagac ctccagtcgg aaaaagtgaa acaggcaaaa tggtggacgc tgcaacaaat
    30241 tgaagaaaat ttgcaggaag gaatttttac cgaaaacttt ctgatagaat ttgatttgtt
    30301 gaaaaagagt ggagttttgg agaataatat ttgcgattgt gcgtgcaaat tgaaagaaac
    30361 cattttcaaa gctcctcatc tgtttcggta aaagcaaagc ataaataact taatgatgag
    30421 attatatttt attttgctcc taatagcatt tgtttcctgt ggtgggcatt ccaactcgga
    30481 atttaagctg caaaattcct ttcaaattaa gctgcaggat tcgatttgta tcaatgctaa
    30541 agattgtttt tattttagta ccgacagtaa cctttatcgg atctttatgt atttttcaaa
    30601 tgcagaattg aaagaaaaaa aaataattga taccgtagat tttagcccat ataaatcaaa
    30661 gatacatagc tttcaatcgg aaaataatga gtcttatgtt gttttatggg aaacagaata
    30721 cgaattttat cctttaattt atgtctactt tattacagca ggtaaaatag taaaaattgg
    30781 agaactttta atttctttac cctgtcagtc gtgtgagagt ttcgaatatc cattaaaaga
    30841 tattcgaatg cttcaaaagg gaaagaatat tgaaatctca tttttaaaag atgtaaatta
    30901 taagccaagt aacggtaatg aatggcattt gtataaagcc ggtatgttaa aatgcatttt
    30961 taatgctgaa acaaacaaat taaaatagat ctcatcattg aattagtgtt aagttagatg
    31021 acaaaaacaa tttatttcac ttaaagataa acctatatta ttgattatct atgttttaca
    31081 ttatataaag atattttgtc tctcgctggc ttcagtgatt taccactgcc aaaatcccct
    31141 gatttgtaag gttttggaga gtggaaggag tgttcttgtt attaaggttt gagcaaaata
    31201 aggttgaagg aaatac
//
LOCUS       DAOSIB010000017        30277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_1712_length_30277_cov_11.417014, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000017 DAOSIB010000000
VERSION     DAOSIB010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 30277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30277
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1712_length_30277_cov_11.417014"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1941
                     /locus_tag="PLD44_02840"
     CDS             <1..1941
                     /locus_tag="PLD44_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOF98307.1"
                     /translation="MSYLSFDKSLLINLERSLSKEILRTNRAGTYNSSTLIDCNTRKY
                     HGQLVVPVPELGEANHVLLSSLDETIVQRGIEFNLGVHQYEGNNFYPNGHKYIRQFDC
                     EIIPRTIYRVGGVILSKERMLVSFEPRVLFKYTLLEAHSPTLLRFKPFLAFRNVHELT
                     HQNPEANCDFQPVSNGIAMKLYENYPTLFMQLSKKNVYQHFPDWYKNIEYYKERERGF
                     EYREDLLVPGSFEIPIKKGESVIFSAGISEVSTSSLKKLWDKELEKRNPRCDMFSALK
                     NSAQQFYKREGDKTYLLAGYPWFTARARDEFVALPGCTLSIGRMDYFDAIIETAIPEI
                     ESFINENKVSSKLKEIDAPDVLLWFVWTIQQYARHTSLSETAQRFGKLVASIISFVRK
                     QRHPNLFLHNNGLLYVNGLEKPATWMNAVENGRPITPRTGYVVEINALWYNALKFSSQ
                     LLGEMGEEMEANVIDYQSGILRESFIKTFWNGNYLYDFVNGNYNDKEVRPNMIYAVGL
                     PFSPLEKSQQKLILDIVTKELLTPKGLRTISPKSGMYRPVYIGGQLERDRNYHNGPVW
                     PFTFGAFVEAYLKIYKMSGKSFIERMMIGFEAEMKELCVGTLSELYDGNPPFKGHGGM
                     SFAMNVGEVLRVMEILKEYKED"
     assembly_gap    2043..2142
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2164..3420
                     /locus_tag="PLD44_02845"
     CDS             2164..3420
                     /locus_tag="PLD44_02845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOF98308.1"
                     /translation="MKVLMFGWEFPPHILGGLGTASYGLVKAMAHQPDMEITFVIPKL
                     YGDEDQSFLKIIGACYVPVVWKENDWNYVNERIGWIMNPDEYFWLRNGIKYDFRRIYT
                     NDLGCIEFSGRYPDNLLEEISNYEAVASVLAHQLSFDVIHSHDWLTYPAGIFAKQISG
                     KPLVVHVHATEFDRSRGNVNPIVYDIEKRGMDAANHIITVSNLTRNIVIEKYFQDPKK
                     VTTVHNAVEPLPDVDSYVKTPRSDKIVTFLGRITMQKGPEYFVEAANRVLKKNKKVRF
                     VMAGNGDMMQDMINLAAKRGITDRFHFTGFLKGRQVYQMLADSDVYIMPSVSEPFGIS
                     PLEAMQVGTPTIISKQSGCSEILTHSIKIDYWDIDGMADAVNAIIRNPAMYKSLHELG
                     REEVNNIRWEDAGKKVIKIYNSLLGK"
     gene            3602..4888
                     /locus_tag="PLD44_02850"
     CDS             3602..4888
                     /locus_tag="PLD44_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOF98309.1"
                     /translation="MKNLCFYFEIHQPMRLKRYRFFDIEQDHYYYDDFQNEERIRMLA
                     EQSFLPANRTLLEIIQNSNGKFRCAFSFSGIVLEQLEQYAPEVIDSFEELSKTGCVEF
                     MAETYAHSLSSVYDIHEFEQQVALQTEKIESLFGKKPTVFRNSELIYSDEIGEKIYNM
                     GYRTILIDEVSHVLGWKSPNFVYRHPTLPKLKLLVRNFKLSDDIAFHFSDRSWSDYPL
                     TADKFISWIKNMPDDQPVVNIWLGYEALGLFQRPESGIFEFLKAIPFFALQNNIGFLM
                     PSEVAKKIEAVDILSSPEPISWSGIEKDISLWNGNDLQQEALEKLYAVGERVRLCTDR
                     PLRHDWLMLQSSDHFRYMSHKDAFGTYYESPYDAFMNYMNILADFLLRVDEQYPTTID
                     NEELNALLNTIYELEKEVERLENELKKLRSRKKTEN"
     gene            4900..5487
                     /locus_tag="PLD44_02855"
     CDS             4900..5487
                     /locus_tag="PLD44_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98310.1"
                     /translation="MVHEFGKSLHYEKSQFRRADAFYREVLLVDEIIRFATDSKEDMK
                     MQLQDIDVQLIKTNRVCHVSEKFREKDFVDLYVEVFSKYPHTRGWLETGLADMLVYFV
                     PEVVYWCNAKNLRKFCVDMLFPMIPAEWYKEIYCSATSIISKNMNFFDQQTIVHLIKA
                     NNQTSDGNRWCTIGISVPFEFLKNNGLNIRKYLFP"
     gene            5640..6359
                     /gene="hisA"
                     /locus_tag="PLD44_02860"
     CDS             5640..6359
                     /gene="hisA"
                     /locus_tag="PLD44_02860"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOF98311.1"
                     /translation="MEIIPAIDIIDGKCVRLSQGNYDKVTLYSRYPDEIAKMFADEGF
                     RRLHLVDLDGAKAQHIVNYKVLEKITSTTDLVVDFGGGLKSDDDLRIAFECGASMVTG
                     GSIAVKNPEIFVGWLEKYGGEKIILGADVKNEKIAVSGWTESTELELIPFVHNYFSKG
                     VKYVICTDISCDGMLQGPAIELYKKIISAEPQVHLIASGGVGSLHDIEMLNEAGIPAV
                     IVGKAIYEGNISLKELSRFLF"
     gene            6405..6863
                     /locus_tag="PLD44_02865"
     CDS             6405..6863
                     /locus_tag="PLD44_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HOF98312.1"
                     /translation="MLKEILYVSGKPGLYKMVSQGKNLMVIESLSDGKRIPVFSSDKL
                     VYLKDIAIFTENGEIPLSEVFEKIKVKENGAKCAVDAKSGNEKLVQYLQEILPDYDKN
                     RVYPSDIRKLINWYNILVDAKLTDFAASETETGDKVETVSDKTEEISSEK"
     gene            6878..7972
                     /locus_tag="PLD44_02870"
     CDS             6878..7972
                     /locus_tag="PLD44_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOF98313.1"
                     /translation="MKVQTVCNILEEFAPLALQEAYDNAGLIVGDPFMEVNGILISLD
                     VTMEVLEEAKRKKCNFILAHHPLIFNSLKRLVGQNETERCVIFALKHDLAIYAGHTNV
                     DNLWNGVNQKIAEKLQLVHTKVLKPLSHQLLKLVTFVPHAHADKVRKAMFEAGAGSIG
                     NYDSCSYNVEGTGTFKAQENAHPFVGEHNEFHAEPETRIEVILPSYLKNSVVNALLQA
                     HPYEEPAYDLIPLENEWNRAGAGLLGNLEIPTDELAFLHRLKTVFSVPCIRHTPLLGK
                     NIVKVAVCGGAGSAFLKDAVQQGADIYISADFKYHEFFNAEGKILIADVGHYESEQFT
                     KDIFYEIISKKIPNFVVKISETYTNPINYL"
     gene            7978..8754
                     /locus_tag="PLD44_02875"
     CDS             7978..8754
                     /locus_tag="PLD44_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOF98314.1"
                     /translation="MATEPKTEKEISVEEKLRALYELQRVYSKIDEIRNLRDELPLEV
                     QDLEDEIIGLNTRLENYKNEIAELEAAIKAKKLEIEQARTKIEKYKEQQENVRNNREY
                     DNLSKEIEYQTLEIELCEKRIKEYTAALEAKKAEQQSTMETLSERRLDLEQKKGELNE
                     IISETKQEEERLREQIKQIEQRVEPRLLTAFKRIRKNSRNGLSVVPVQRDACGGCFNK
                     IPAQRQLDIRSRKKIIVCEYCGRILVDPELAGIVPPTSES"
     gene            9685..10170
                     /locus_tag="PLD44_02880"
     CDS             9685..10170
                     /locus_tag="PLD44_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOF98315.1"
                     /translation="MDLKDRIEKIMQNEAMNAVQFASEIGIQGSTLSHILNGRNKPSL
                     EVMMKILKRFDYINSDWLIFGEGPMNRTERQSKTPTLFDSQDLSNTNTVNYTQKTEQK
                     IESLQSHFQQKPEQKEIISESVNSKISEENKIAPVVVEKIIKRIIVYFNDNTFQEFTT
                     T"
     gene            10276..11049
                     /locus_tag="PLD44_02885"
     CDS             10276..11049
                     /locus_tag="PLD44_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOF98316.1"
                     /translation="MKKLILDLILKKNIRLNEQVYLLIFSSEKGLPEILPGQFAQVKI
                     NDSPATFLRRPFSVNFVDYENNELWLLIQVVGAGTLKLSETSPGASVNMILPLGNSFS
                     FPDEKKDEKILLIGGGSGIAPLLFFGSWLFDAGFSPEFLIGARSSIHLVEVDEFKKYG
                     NVYITTEDGTEGEKGFVTDHSILKKRNYDRIYSCGPTPMMKAVGAYAENNNIFCEVSL
                     ENTMGCGIGACLCCVTDTTQGRVCVCTEGTVFNIEKLKW"
     gene            11043..11954
                     /locus_tag="PLD44_02890"
     CDS             11043..11954
                     /locus_tag="PLD44_02890"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOF98317.1"
                     /translation="MVDLKVDVGKLHLKNPVMTASGTFGYGMEYSDFIDISRIGGIIV
                     KGTTLRKREGNPYPRMKETASGMLNAVGLQNKGVDHFIEHIYPQLTNVDTNIIVNVSG
                     STIEEYVETAEKLNILEKIPAIELNISCPNVKEGGMLFGTSCTAAKEVVAKVRRAYKN
                     VLIVKLSPNVTNISEIAQAVEEAGADAISLINTVLGMAIDAETRKPCLSTVTGGLSGP
                     AIKPLALRMVWETARDVKIPVIGIGGIMNAADAIEFMLAGASAVQVGTANFIDPTVTI
                     KIIEGIEDYLNRHGYTSVSDIVGALET"
     gene            complement(11984..12442)
                     /locus_tag="PLD44_02895"
     CDS             complement(11984..12442)
                     /locus_tag="PLD44_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOF98318.1"
                     /translation="MLQLNLPSYNFKIKKVNETLYIFDSQRKKYVKLTPEEWVRQNFI
                     RFLLEEKHFPSAYLAVEKELVFNGMKKRCDAVLYDGQAQPLMILEFKAPHVPLTQSVF
                     DQVAVYNYRLNVEYFIVSNGLEHYCCRVMKEHTKFEFLKEIPDYTNFLNQ"
     gene            12525..13298
                     /locus_tag="PLD44_02900"
     CDS             12525..13298
                     /locus_tag="PLD44_02900"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOF98319.1"
                     /translation="MKTKKEIVENWLPRYTKRPLEEFTPYILLVNFNIYVELFAGYYQ
                     VPIVGRDGNMPNASANGITIINFGLGSPNAALIMDILSAIQPKAVLFLGKCGGLRDKN
                     KEGDYILPIAAIRGEGTSNDYFPPEIPSLPAFSLQRSVSSNIRDIGKDYWTGTVYTTN
                     RRVWEHDEKFKEYLRMTRAMAIDMETATLFSSGFYNGIPTGALLLVSDMPMYESGIKT
                     AESDKRVTANFVEEHLRIGIESLTSIMHNSRTIKHLRFE"
     gene            13342..14358
                     /gene="holA"
                     /locus_tag="PLD44_02905"
     CDS             13342..14358
                     /gene="holA"
                     /locus_tag="PLD44_02905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOF98320.1"
                     /translation="MIKQEAAADEILQEIKSKNFHSVYFLMGEEPYYIDKISNYIQEN
                     VLSESEKDFDLTILYGKDVEISTVINTAKRFPMIAPYQVVIVKEAQMIISWENLVYYL
                     QKPLKSTILVFCYKYGTPDKRTKWFQETKKAGVVFESGKLYDYQVGEWIRSYLKTRKT
                     GITQQAENMLVDYLGTDLTKIANELDKLLLTKPQDTQQITPELIERNIGISKDYNVFE
                     LQKAFVNRDILKANRIILYFAENKKNNPLVLVLAQLFNFFGNLLLFHYMKDKTEKNVA
                     SELKINPFFVKDYQNAAKIYDREKTIKVLSLIRETDARSKGVDNAGIDEGELLKELVY
                     KILH"
     gene            complement(14373..15263)
                     /gene="lgt"
                     /locus_tag="PLD44_02910"
     CDS             complement(14373..15263)
                     /gene="lgt"
                     /locus_tag="PLD44_02910"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOF98321.1"
                     /translation="MLNYIVWNVNPEIFRLGSLSVRWYGLLWAVGILLALLIGQKIFK
                     HEKLPEVWLDKIFIYAVIGGIVGARLGHCLFYEWKLLPEPVQFLGITFKYGNYYLSHP
                     WEILYVWHGGLASHGGAIGILIAMYFYNKNVTKKGYLWAFDRLAIGVAITGACIRLGN
                     LMNSEIYGNPTNLPWGFIFVRAGETQPMHPTQIYEMLYCLITFGVIWWLYWKKEAYKR
                     TGFIFGVFLIGIFGTRFLLEFIKLDQEAFESGMLLNMGQILSIPFIVWGIWLVARKQK
                     ISSTSNVNANKNKPVANRKK"
     gene            complement(15295..18597)
                     /gene="secA"
                     /locus_tag="PLD44_02915"
     CDS             complement(15295..18597)
                     /gene="secA"
                     /locus_tag="PLD44_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOF98322.1"
                     /translation="MALNNFFKKILGNKAQRDLREIEPIVEKIQAVYPEIKSLSNDDL
                     RARTASLKERIQEYVAPEVNRIQELKNSIEETEIDLREKIYTEIDKLEKEITDKQEKI
                     LNEILPEAFNIMKETARRFAENDEIIVKANDFDRNLAAKHDFVYIEGEEAHYKNEWLA
                     GGSMIKWDMVHYDVQLIGGVVLHQGKIAEMATGEGKTLVATLPVFLNALTGNGVHIVT
                     VNDYLSKRDSEWMGPLFMFHGLSVDCIDKYRPNSEERRQAYLADITYGTNNEFGFDYL
                     RDNMATSPLDLVQRKHHYAIVDEVDSVLIDDARTPLIISGPVPKGEDQLFEEFRPTVE
                     RLVNAQKVLATKYLTDARNLLKSENEKEREEGALALFRSYKGLPKNKALIKFLSEPGN
                     KTILLKTEEYYMQENNRNMHIATDPLYFVIDEKNNSIELTDKGFDLLSETSEDPTFFV
                     LPDVGSEIAELEKNHTLTPEEKQAQKDEILQNYSIKSERIHTVNQLLKAYTLFEKDVE
                     YVVINNEVKIVDEQTGRIMEGRRYSDGLHQALEAKERVTVEAATQTFATITLQNYFRM
                     YHKLAGMTGTAETEAGEFWDIYKLDVVVIPTNRPVIREDMEDRVYKTKREKYAAVIEE
                     IVRLMEAGRPVLVGTTSVEVSELLSRMLTGRKIKHNVLNAKQHQREAEIVAEAGRKGT
                     ITIATNMAGRGTDIKLTPEVREAGGLAIVGTERHESRRVDRQLRGRAGRQGDPGSSVF
                     YVSLEDDLMRLFGSERISGVMDRLGFQEGEMIQHPMISRSIERAQKKVEENNFGIRKR
                     LLEYDDVMNSQREVIYTRRRHALMGERIESDITNMIYDAIENMVANAKSNGFYEELEE
                     QLLTVFAMDVPFSEEEFNSARTRDLTDKVVNAALTTFKRKMEKLSSIAYPVIKQVYEQ
                     QGDKYENILIPVTDGKRIFNVVVHLETAYKTEGKEVIKSFEKQTLLYVIDDEWKEHLR
                     QLDELRNSVQNATYEQKDPLLIYKLESFGLFKEMIENINHKVLSILMRGQIPVREPEK
                     VREAKIPARQDFSKYKTQKDEIAERGNSDAGDTREKQRPQPVHVEKKVGRNDPCPCGS
                     GKKYKHCHGANA"
     gene            complement(18808..19554)
                     /gene="fabG"
                     /locus_tag="PLD44_02920"
     CDS             complement(18808..19554)
                     /gene="fabG"
                     /locus_tag="PLD44_02920"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOF98323.1"
                     /translation="MKLLEGKVAIVTGAARGIGKSIAIKLAEEGADIAFTDLVIDENA
                     SNTQKEIESLGVKVKAYASNAANFEETHAVVEEIQKEFGHIDILVNNAGITRDGLLIR
                     MTESQWDSVINVNLKSAFNFIHACAPIMMKQRNGSIINMSSVVGVSGNAGQANYSASK
                     AGLIGLTKSVAKEFGSRGIRANAIAPGFIETEMTHALTEEQRAKWVENIPLRRGGTPE
                     EVAKVTLFLASDLSSYVSGQVIHVCGGMNT"
     assembly_gap    19648..19747
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19918..20772)
                     /locus_tag="PLD44_02925"
     CDS             complement(19918..20772)
                     /locus_tag="PLD44_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOF98324.1"
                     /translation="MNKLFRLGIVSFLIVSLFTSCEKENNQVTSVTISETSKTMHIGD
                     IDTLYAQSQYEGNIVPSLAWSSSDSSVVSVDKNGEISAQKKGNAIITASAGDKSATCN
                     VTVDDEIVTSFTKAELAFWGDYYQTGNSNNYSLYLTNRQDTLLIEINTDTLSTNGIPS
                     GTYEMIENFDSLQQIVPLTLLPAFIYDGYSYGSFLFNSVAVYPIYSGIATFSNEENSN
                     TITYNFLDYFGNHIAGEYSGTIIFYDETENSSAVASNVKAIKMKKNIQQFSSNRKISK
                     ALKLINAR"
     gene            complement(20831..22693)
                     /gene="rho"
                     /locus_tag="PLD44_02930"
     CDS             complement(20831..22693)
                     /gene="rho"
                     /locus_tag="PLD44_02930"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOF98325.1"
                     /translation="MSNYNILQLNAKELSELKQIASELGLKVPGTITKDKLVYEILDQ
                     QAIANAQQKVASNEKKENERKKRMRVSVKKNVEQKVFSSPNPTITPAPSNSTTSPTPL
                     VPFTENSPKQEIQNNQTSDNTIEAPASTTEVKPKKSGGQSKRNKTSKSNQKTLLKKTS
                     AETSEKPEVNIPLEEIDNFEVPNLPEVEITEEQDIDIEKDTLIPVQIIETSEPRPGLS
                     LNINKNANGNNNENKVKIAPRQDKTEKQFDFDGILEGTGTLEIIQDGYGFLRSPDYNY
                     LSSPDDIYVSQSQIKLFGLKTGDTVTGTIRPPKEGEKYFPLIKVLKINGRSPEYVRDR
                     VSFEHLTPLFPNEKFKLTTGVNDPLSVRIVDLFSPIGKGQRGLIVAQPKTGKTVLLKD
                     IANAIAANHPEVYMIVLLIDERPEEVTDMARSVKAEVISSTFDEPAERHVKVASMVHE
                     KAKRMVECGQDVVILLDSITRLARAYNTVAPASGKILSGGVDANALHKPKRFFGAARN
                     IENGGSLTIIATALTETGSKMDDVIFEEFKGTGNMELQLDRKLANKRIFPAVDILASS
                     TRREDLLLDQDTLNRMWILRRHLSDMNSLEAMEFLKERMERTVNNEEFLVSMNA"
     gene            complement(22922..24250)
                     /gene="tilS"
                     /locus_tag="PLD44_02935"
     CDS             complement(22922..24250)
                     /gene="tilS"
                     /locus_tag="PLD44_02935"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOF98326.1"
                     /translation="MILKKVELYIEKNKLLFPEKGKILVGVSGGRDSVALLDILIKLG
                     YRCTVAHCNFHLRGEESDRDEKFVQQLSFNLNIPYYSVDFDTVNYAKQKNISIEMAAR
                     ELRYSWFTSLAEKINAQAIAIAHHADDNVETLLMHLVRGTGLKGLTGISPKNGLIVRP
                     LLCCTRNEINEYIKNNNLSFIEDSTNQSVDFQRNKIRLQVIPLLEEINPSVKKVLSES
                     IERFSEINTFYENAIEKIKKQLLTVDNDQLKINIDLLCKQASPKTILFEILHPYGFNE
                     SIVQDIEKHLHDESGKIFYSPTHYLIKDRKYLIISNKIKKNETTFFITENDSEISFPI
                     QLSITRKKKEQNFVISKDNRIIQIDASLIQYPLTLRRWTNGDTFFPFGMNRQKKLSDF
                     FIDQKLNLKQKAETWLLLSQNQIVWVVGLRLDNRFKITESTQEVLEIKML"
     gene            24403..24945
                     /locus_tag="PLD44_02940"
     CDS             24403..24945
                     /locus_tag="PLD44_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOF98327.1"
                     /translation="MESFLDLVKRRQSAHSFTNKPVEKEKIDRILEIVRLSPSACNAQ
                     PWKFIVVTDEEKRQKIADATSKRFLPVNHFSKQAPVLIVIVEESTNFFTKVGCYGVNR
                     QFPLIDIGIATAHLCLAATNEGLDTHVIGWYNEKKIQQALEIPENKKVMLVVALGYSD
                     EEVRPKKRKKIEELVSYNKY"
     gene            25005..26306
                     /locus_tag="PLD44_02945"
     CDS             25005..26306
                     /locus_tag="PLD44_02945"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOF98328.1"
                     /translation="MNYEQTLDYLYSRLPVFHQIGSAAYKPGLENTIRILTALHQPQN
                     SFRSIHVAGTNGKGSVSHFLSSILQSSGYKTGLYTSPHLVDFGERIRVNGEMIDKQFV
                     IDFVENHKKLIEEVQPSFFEITMAMAFDYFASSKVDVAVIEVGLGGRLDSTNIIQPEL
                     SVITNISFDHEEFLGHTLQEIATEKAGIIKPHTPVVIGEALPETKPVFLQKAQEMNAP
                     IFFSENIKQIFFDRYEENRMIVKTSDEKSYAVGLTGNYQLKNIATVLTAVDQLRKANF
                     EIPEASLQEGLEKVIEKTGLQGRWQIISASPKIIADTGHNPGGIAFVAEQLKTQHYQT
                     LRIIFGMANDKDIDTVLSLLPKNGVYYFTQANIKRAFPSDQLLKKGQQAGLYGKSFSN
                     IEEAIKTALADANKEDLIFIGGSNYVVGEALTFFEKHEEVT"
     gene            complement(26338..26889)
                     /gene="def"
                     /locus_tag="PLD44_02950"
     CDS             complement(26338..26889)
                     /gene="def"
                     /locus_tag="PLD44_02950"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOF98329.1"
                     /translation="MILPIYTYGNPVLRKVAQPIDASYPELKSLVNNMFETMYHADGV
                     GLAAPQVGLPIRVLVIDLAPFKDEDAELGSFKIAMINPTILERSAELLPGEEGCLSIP
                     GIHETVFRSKTIKVHYFDTDFNEYEEIFEDYKARVIQHEYDHLEGNLFTDRVNPLRRQ
                     LLKSKLTNISKGKIQCNYKIITA"
     gene            complement(26917..27351)
                     /gene="ruvX"
                     /locus_tag="PLD44_02955"
     CDS             complement(26917..27351)
                     /gene="ruvX"
                     /locus_tag="PLD44_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOF98330.1"
                     /translation="MGRILAIDYGQKRVGIAVSDVMHISANGLTTLPVNEVFPFLEKY
                     LVQENVETIVVGFPRQMNGKESDSMKFIRPFVAKLVQKFPQIELIYEDERFTSVLAHK
                     TMLEGGLKKKDRQNKALVDKISATIILQSYLESVRYKNERNI"
     gene            27586..29334
                     /gene="aspS"
                     /locus_tag="PLD44_02960"
     CDS             27586..29334
                     /gene="aspS"
                     /locus_tag="PLD44_02960"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOF98331.1"
                     /translation="MYRTHNCGELRISHVGQIITLAGWVQRIRKMGGMVFIDLRDRYG
                     ITQLVFNQEINSDLWEKANKLGREFVVQVTGKVSERSNKNPNLSTGDIEILVSELTVL
                     NESKTPPFTIEDATDGGDDLRMKYRYLDLRRNVVRKNLELRHQMTIETRQYLDNLGFL
                     EVETPVLIASTPEGARDFVVPSRMNPGEFYALPQSPQLFKQLLMVGGFDRYFQIVKCF
                     RDEDLRADRQPEFTQIDCEMSFVEQEDVLQTFEGLVKHLFKTIKNIDFPEPFPRMEWS
                     EAMKRFGSDKPDIRFGMELVELKEMVTGHDFSVFDNAEYVGGICAPACASYTRKQIDE
                     LTEFVKRPQIGAKGLIYIRFENDGSFKSSIDKFFSVDDLKKIATLFSANPGDLILIMS
                     GEKLKTQKALCELRLEMGGRLGLRKKDEFAPLWVIDFPLFEWNEELNRYQAMHHPFTS
                     PKPEDIPLLDTQPGEVRANAYDMVVNGVELGGGSIRIHNSNLQNKMFELLGFTPEKAQ
                     AQFGFLLNAFQFGAPPHGGLAFGLDRFVSLLAGLDSIRDCIAFPKNNAGRDVMINAPS
                     PLDPSQLEELHLMLKL"
     gene            29347..29604
                     /locus_tag="PLD44_02965"
     CDS             29347..29604
                     /locus_tag="PLD44_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98332.1"
                     /translation="MNEKQTKNAAESIVKALVELSLGKEPNIFSKSPFRKLAEHKNYT
                     LIRDAYIDYLKEFDGKIDSDEDMKRLFDFRIKILNYFNDDK"
BASE COUNT         9472 a         5200 c         5679 g         9726 t
ORIGIN      
        1 atgagttatc taagtttcga caaatccttg ttaattaacc ttgaacggtc tctttcgaag
       61 gaaattctcc gtaccaatcg tgcaggcact tacaacagtt caaccttgat cgattgcaac
      121 accagaaaat atcatgggca attggtagtg cccgtacccg agttgggcga agccaatcat
      181 gtattgctct cttcgttgga cgaaaccatt gttcaacgtg gaattgaatt taatttgggg
      241 gtgcatcagt acgaaggcaa caatttttat cccaacggcc acaaatacat tcgtcaattc
      301 gattgcgaaa ttattccaag aacaatttat cgtgtggggg gagtaattct ttcaaaagaa
      361 cgcatgctgg tttcatttga accgcgtgtc ctttttaaat atactttgtt ggaagctcat
      421 tcgccaactt tgttacgctt taagcctttt ctggcttttc gaaatgttca tgaacttacc
      481 catcagaatc cagaagccaa ctgtgatttc caacctgttt ccaacggtat agccatgaag
      541 ctttatgaaa attatcctac gcttttcatg caactttcca aaaagaatgt ttatcagcat
      601 ttccccgact ggtataaaaa tatagaatat tacaaagaaa gggaaagagg ttttgaatat
      661 cgagaagact tgttggtgcc gggttctttt gaaattccca taaaaaaagg agaatccgtt
      721 attttttcag caggaatatc ggaagtctca acttcatcac tgaaaaagct ttgggataag
      781 gaattggaaa aaagaaatcc acgctgtgat atgttcagtg cactgaaaaa ttctgcccaa
      841 caattttata aacgcgaagg cgacaaaact tatttattgg caggttatcc ctggtttacg
      901 gcaagggcaa gagatgaatt tgttgcttta cccggttgta ctttgtcgat tggcagaatg
      961 gattatttcg atgccataat tgaaacagcc attcctgaaa ttgaaagttt tattaacgaa
     1021 aataaagtca gctccaaact gaaagaaatc gatgcacccg acgttttgct gtggtttgta
     1081 tggaccattc agcaatatgc tcgtcacact tcactttcag aaacagcgca gcgtttcgga
     1141 aaattggttg cttcaattat ttcttttgtt cgcaaacaac ggcatcccaa tctttttctc
     1201 cacaacaacg gcttgcttta tgtcaatggg ttggaaaaac ctgctacatg gatgaatgcg
     1261 gtagaaaatg gcagacccat tactcctcga acaggttacg tagttgaaat taatgcactg
     1321 tggtataatg ctttgaaatt ttcttctcaa cttcttggtg aaatgggaga ggagatggaa
     1381 gccaatgtaa tagactatca gtcaggcata ttaagagaat cgttcataaa aactttttgg
     1441 aatggaaact atctttacga ctttgttaac ggcaattaca atgacaagga agttagacca
     1501 aacatgattt atgcagtggg cttaccattc tctcctcttg aaaaatcaca gcagaagttg
     1561 attctcgata ttgtaaccaa agaattgttg acaccgaaag gattaagaac cattagccct
     1621 aaaagtggta tgtatcgtcc ggtgtacata ggtggacagt tggaaagaga ccgaaattac
     1681 cacaacgggc ctgtttggcc ttttactttt ggtgcatttg ttgaagctta tttaaaaatt
     1741 tacaaaatga gtggaaaatc ttttatcgaa agaatgatga ttggcttcga ggcagaaatg
     1801 aaagaattgt gtgtcggtac actttccgaa ttatatgacg ggaatccacc ttttaaggga
     1861 cacggcggaa tgtcttttgc catgaatgtg ggagaagtat tgcgtgtaat ggaaattttg
     1921 aaagaatata aagaagacta ataccactga atttggtgta aacggaaagt ttagaggtga
     1981 gtatatacga tgaaaaactg aagaatgtat gaaagtacta atgtttggct gggaatttcc
     2041 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgatgaaa aactgaagaa
     2161 tgtatgaaag tactaatgtt tggctgggaa tttcctcctc atattttggg cggtttgggt
     2221 acggcttcct atggattggt gaaagccatg gcacaccagc ccgatatgga gatcactttt
     2281 gttattccca agctctacgg agatgaagac caaagttttt taaaaattat tggggcttgt
     2341 tatgtccctg tggtttggaa agaaaacgat tggaattatg tcaacgagcg tattggctgg
     2401 atcatgaatc ctgacgagta tttctggctg agaaatggta tcaagtacga ttttcgcaga
     2461 atttatacca atgatttggg atgtatagaa ttttccggtc ggtatcccga caatttgctg
     2521 gaagaaattt caaactacga ggctgtggca agtgtgttgg cacatcaatt gagttttgat
     2581 gtgattcatt cacacgactg gttgacttat cctgcaggta tttttgccaa acaaatcagc
     2641 ggaaaacctt tagtggtgca tgtgcatgcc accgaatttg atcgcagtag gggaaatgtt
     2701 aatcccatag tttatgatat tgaaaaaaga ggaatggatg cggcaaatca cattatcacc
     2761 gtaagcaatc tgacccgaaa tattgtgatt gaaaagtatt tccaggatcc gaaaaaggtt
     2821 acaaccgttc ataatgccgt agagcctctt cctgacgtag attcttatgt taaaacaccg
     2881 agaagtgata aaattgttac ttttttggga aggataacca tgcagaaagg gcctgagtat
     2941 tttgtagaag cagccaatcg tgtgctgaaa aaaaataaaa aagtccgttt tgtgatggcc
     3001 ggtaatggag atatgatgca ggatatgatt aatttggctg caaagcgagg tattaccgac
     3061 cgttttcatt ttaccggttt ccttaaggga agacaagtgt atcaaatgtt ggccgatagt
     3121 gacgtatata ttatgccttc cgtttctgaa ccttttggca tttctccttt agaagccatg
     3181 caagtgggta caccgaccat tatttcaaaa cagtcgggat gttctgaaat tttaacacat
     3241 tcaattaaaa ttgactattg ggatatcgat ggtatggccg atgcagtaaa tgcaattatc
     3301 aggaatccgg ccatgtataa aagccttcac gaattgggga gagaagaagt aaacaacatt
     3361 cgttgggaag atgcaggtaa aaaagtaatt aaaatataca atagtctgtt ggggaaataa
     3421 attcctttaa agaaattttc ttttttagaa atcaacctca ataagaaaat tgtcttattg
     3481 ataatcaaat ttataaatta aaccaatctt tatcgtttga atagatttac acaatttttg
     3541 gagctgaaat gtctgaaaaa agtaaatcca atttcattcc taaagaaaaa aggataaatg
     3601 tatgaaaaat ctgtgttttt attttgaaat tcatcagcca atgcgattaa aaagatatcg
     3661 tttcttcgac attgaacagg atcattatta ttatgatgat tttcagaatg aagaacgaat
     3721 tcgtatgttg gctgaacagt cgtttttgcc ggccaatcgc acgcttttgg aaattatcca
     3781 aaattccaat ggaaaatttc gttgcgcttt ttcgttttcg ggaattgttt tggaacagct
     3841 ggaacagtat gctcccgaag taatcgacag tttcgaagaa ctttccaaaa ccggctgtgt
     3901 ggagtttatg gccgaaactt atgctcattc tctttcatct gtttacgata tacatgaatt
     3961 tgaacagcaa gtagccctgc aaaccgaaaa aatagagtca ctttttggaa aaaaaccgac
     4021 tgtattccga aattcggagt tgatttactc ggacgaaatt ggagaaaaaa tctacaatat
     4081 gggatataga accatattga ttgatgaagt cagccatgtt ttgggttgga aaagtccaaa
     4141 tttcgtttat cggcacccga cgttaccaaa attaaaattg ctggttcgta attttaagtt
     4201 gagcgacgat attgcttttc atttttctga tcgctcgtgg agcgattacc ctcttacagc
     4261 cgacaaattt atttcatgga taaaaaatat gcccgatgat cagcctgttg taaatatctg
     4321 gcttggctac gaagcattgg gtttgtttca gcggcctgaa agtggaattt ttgaatttct
     4381 caaagctatt cctttttttg ccttgcaaaa caatatcggt tttttaatgc cttcggaagt
     4441 ggcaaaaaag attgaagcag ttgatattct ttcttctccc gaacccatta gttggtccgg
     4501 aatagaaaaa gacataagtt tgtggaatgg aaacgacctt cagcaggaag cactcgaaaa
     4561 actttatgcg gtaggcgaga gggttcgcct ttgtaccgac agaccgcttc gacacgattg
     4621 gcttatgttg cagtcatccg atcattttcg atacatgagt cataaggacg cttttggaac
     4681 ttattacgaa tcgccctatg atgcgtttat gaattatatg aatattttgg ctgattttct
     4741 gctgagagtt gatgaacaat atcccactac tattgataac gaagaactta atgctttgct
     4801 caataccatt tacgaactcg aaaaagaagt tgaacgtttg gaaaacgaat tgaaaaaatt
     4861 gcgttcgaga aaaaagactg aaaattaaaa ttgattttca tggtgcatga gtttggaaaa
     4921 agtcttcatt acgaaaaaag tcaatttcgg cgcgccgatg cgttttatcg tgaagtttta
     4981 ctcgtagatg aaatcatacg atttgcaaca gactcgaaag aagatatgaa aatgcagctt
     5041 caggatatcg acgtgcaact gataaaaacc aatcgagtct gccatgtttc cgaaaaattc
     5101 agagagaagg atttcgtcga tttgtatgtt gaagttttta gcaaatatcc tcatacgcgc
     5161 gggtggctag aaaccggttt ggccgatatg ttggtgtatt ttgttcccga agtcgtctat
     5221 tggtgcaatg ctaaaaatct cagaaaattt tgtgtggata tgctgtttcc aatgattcct
     5281 gctgaatggt acaaggaaat ttattgttcc gctacttcga ttatttccaa aaacatgaat
     5341 tttttcgatc agcaaaccat tgttcatctg atcaaagcaa ataatcaaac atctgatggg
     5401 aaccgttggt gtaccattgg aataagtgtt ccttttgagt ttctgaaaaa taacggatta
     5461 aatatcagaa aatatttgtt tccgtaattt tttgagtgat gaatttttta gctgtttatt
     5521 ttgtagaaat atcgattatt cacagctata atcaaaatag ttgttgactt gtttcacatt
     5581 ttcttttttt atacttttgc caacttgttt gaagaaaata gatacaaaaa acatcataaa
     5641 tggaaattat tccggccatt gacattattg acggtaaatg tgttcgcttg tctcaaggca
     5701 actacgataa agtaacgctc tacagccgct atcccgatga aattgctaaa atgtttgctg
     5761 atgaaggttt cagacgattg catttggtag atttggatgg agcaaaggct caacacattg
     5821 tcaattacaa ggtattggag aaaattactt ctactaccga cctcgtagtt gatttcggcg
     5881 gaggattgaa gtcggacgat gatttgcgta ttgcttttga atgtggagct tcaatggtaa
     5941 cgggaggaag cattgccgta aaaaatcctg aaatcttcgt tggctggctc gaaaaatacg
     6001 gcggagaaaa aattattttg ggagctgatg ttaaaaacga aaaaattgcc gtatcgggct
     6061 ggactgaaag tactgaattg gaattgattc cttttgttca taattatttt tcaaaaggag
     6121 ttaaatacgt tatttgtact gacattagtt gtgatggaat gttgcaagga ccggcaattg
     6181 aactctacaa aaaaataatt tctgccgaac ctcaggttca tttgatagca agcggtggag
     6241 tgggttcact tcacgatata gaaatgttaa atgaagcagg tattccagca gtcatagtcg
     6301 ggaaagcaat ttacgagggc aatatttcat taaaagagtt aagtcgattt ttattttaaa
     6361 ttttaaacat taaaatattt taaattattt gtaatcaata aattatgttg aaagagattt
     6421 tatatgtttc cggcaaaccg ggtctttaca aaatggtatc acagggaaaa aatctcatgg
     6481 ttattgaatc cctgagcgat ggaaaacgta ttccggtttt ttcaagtgat aaattagtgt
     6541 atttgaaaga tatagctatt tttactgaaa atggcgaaat tccattgtct gaagtttttg
     6601 agaaaatcaa ggtgaaagaa aatggagcaa aatgtgctgt cgatgctaaa tcgggaaatg
     6661 aaaagctggt tcaatacctt caagaaattc ttcctgatta tgataaaaac agggtttatc
     6721 caagcgacat ccggaaatta atcaattggt acaacatttt ggtagatgcc aaattgaccg
     6781 attttgcagc atctgaaact gaaaccggcg acaaggttga aaccgtatca gataaaacag
     6841 aagaaatttc ttcagaaaaa taaattcgtt ttcagccatg aaagttcaaa cagtttgcaa
     6901 tattctggaa gaatttgcac cattggcgct acaggaagct tatgataacg cagggctgat
     6961 agtgggcgat cctttcatgg aagttaacgg tattttgatt agtctcgacg ttacgatgga
     7021 agtattggaa gaagcaaagc gaaaaaaatg caattttatt ttggctcatc atcctttgat
     7081 tttcaattcg ttaaaacgtc ttgttggtca gaatgaaaca gaacgttgcg ttatttttgc
     7141 gttgaaacac gatttggcaa tttacgccgg tcataccaat gtggacaatc tttggaatgg
     7201 agtcaaccaa aaaattgccg aaaaattgca attggttcat acaaaagttt taaaaccttt
     7261 gtctcatcaa ttgctcaagc tggttacttt tgttcctcat gctcatgccg acaaggttag
     7321 gaaagcaatg tttgaagcag gtgccggcag catcggcaat tacgattcgt gcagctataa
     7381 tgtagaagga acaggaactt ttaaagccca agaaaatgcc catccgtttg ttggcgagca
     7441 caatgaattt catgcagaac ccgaaaccag aatagaagta attttaccga gttatttgaa
     7501 aaattcggtt gttaatgcgt tgttacaagc tcatccttac gaggagcctg cttatgattt
     7561 gattcctctg gaaaatgaat ggaacagggc aggagcgggg ctattgggta accttgaaat
     7621 accaacagat gaacttgctt ttctacatcg tttgaaaacc gttttttcgg ttccttgcat
     7681 tcgtcataca ccattgttgg ggaaaaatat tgttaaggta gccgtttgtg gaggagcagg
     7741 aagcgctttt ttgaaagatg ccgttcaaca gggagccgat atttatattt ctgcggactt
     7801 taaatatcat gaatttttta atgctgaagg aaaaattttg attgcagatg ttggacatta
     7861 tgaatctgaa caatttacga aagatatttt ttatgaaatt atctcgaaaa aaattcctaa
     7921 ctttgttgtc aaaatctcag aaacctatac aaatcccatt aattatttgt aatcaatatg
     7981 gctacagaac caaaaacaga aaaagaaatt agcgttgaag aaaagctcag ggcattatac
     8041 gaactacaga gagtgtattc aaaaattgat gaaatcagaa atttgaggga tgaacttccg
     8101 ttggaagtgc aagaccttga agatgaaatc attggtttga atacccgact ggaaaattat
     8161 aaaaatgaaa ttgccgaact ggaagctgcc atcaaagcca aaaaattgga aatagagcaa
     8221 gctcgtacta aaatagaaaa gtacaaggag caacaggaaa atgtcagaaa taatcgtgaa
     8281 tatgacaatc tttccaaaga aattgaatat cagacgctcg aaatagagtt gtgcgaaaaa
     8341 cgtatcaaag aatacactgc agcgcttgaa gctaaaaagg cagaacagca aagcacaatg
     8401 gaaactttat ccgaacgacg cttggatttg gagcaaaaaa aaggagaatt gaatgaaatt
     8461 atttctgaaa ccaaacagga agaggagaga ttgagagaac aaataaaaca aattgaacaa
     8521 cgagttgaac ctcgactttt gacggcattc aaacgcattc gtaaaaattc gagaaatggt
     8581 ctttcggttg ttcccgttca gcgcgatgct tgtggaggat gcttcaataa aattcctgca
     8641 caacgacagt tggatattcg ctcgcgcaaa aaaattatag tgtgtgaata ttgtggccgc
     8701 attctggtcg atcccgaatt ggccggaatt gttcctccga cttcagaaag ctaaaagcaa
     8761 aaaatatttt ttgaaatttt ttaattgcct gagtgatgta cttcattcag gcttttttat
     8821 gtgctgaaat tccattattt cttattccac ataaggaaaa gtacaaatat tgaaatctca
     8881 gagaacaaaa atgattgtat attgctctaa atgatatttt taaattgtat ttttctaact
     8941 gaaaagaatg acaattttat ttcaaagttc aagaatagga gccttgagct atattatccc
     9001 attttcttcc cctctctttc ttaattttta cattgttctt ttagtttttc atttttcatt
     9061 caaagtgtct atatccatcc atgagtagat catagcaaag aaatttttaa atcatctttt
     9121 ttcaacttga ggaatctata catttgtata tttcacaaat atattttacc tatttacaaa
     9181 tgtaattacc tgatttaaat aaatattttc actttggtaa acttaataat ttatataaaa
     9241 caattctata cattcatatt tgtaaaaatt attttgaaca aaaaaattac taataattta
     9301 cattaaacca gataattata ttataatatc tatattttga ctttaataaa atttttactt
     9361 ccataaatat attgtgtcat aatatcgaaa tttacatgtt ttcataatcc atttaattgt
     9421 aaaccaatta tttactatat ataactttgt ttttcgatat aacttaaact attccattaa
     9481 taaaccattt taatgtattg tagccttatt aaatacgact gatttttata cgctgatttt
     9541 tcatttttgt aaatcgataa attcactttt gtaatttaca aaattgatat tacttttgta
     9601 atgaaatttg tttttgtgaa attaatgatt tatctttgta atttcaatca atctcatcaa
     9661 ttctctttaa aattttattg aatcatggat ctaaaagaca gaattgaaaa aattatgcaa
     9721 aatgaagcta tgaatgctgt tcagtttgca tccgaaattg gaatacaagg ttccactttg
     9781 tcgcacatcc ttaatggacg caacaaacca agcttggaag tgatgatgaa aattttgaaa
     9841 cgattcgatt acattaattc tgactggctt attttcggtg aagggccaat gaatcgtaca
     9901 gaacggcaat ctaaaacgcc cactctattc gattcgcaag atttatctaa cacaaataca
     9961 gtaaattata ctcaaaaaac agagcaaaaa attgaatcac ttcaatcgca cttccagcaa
    10021 aaacccgagc agaaggaaat tatatccgag tcggtaaaca gtaaaatttc tgaagagaat
    10081 aaaattgctc ctgtagtggt tgaaaaaatt attaaaagaa ttattgtgta ttttaatgac
    10141 aatacttttc aggaatttac tactacttga gaaaagttga tgtttttcac ggattaattt
    10201 tttcttttaa ttttgcatta tcattgaccg agtttttagg tttaatgtta taacattctg
    10261 ctttacttat tctaaatgaa aaaattgatt ctcgatttaa ttttgaagaa aaatattcgc
    10321 ttaaacgaac aagtttattt gttgattttt tcttctgaaa aaggacttcc cgaaattttg
    10381 cccggccaat ttgctcaggt taaaattaat gattcgccgg ctacttttct ccgacgccct
    10441 ttctccgtaa attttgtaga ttacgaaaat aatgagttgt ggctgctgat tcaagtcgtt
    10501 ggagccggaa cattaaaact gtctgaaact tctccaggag cctctgtaaa tatgatttta
    10561 ccgcttggaa attcgttttc ttttcccgac gaaaaaaaag atgaaaaaat tttgctgatc
    10621 ggtggtggaa gtggtatagc tcctttactt ttttttggat cttggctttt cgatgccggt
    10681 ttttccccag aatttcttat tggcgcacgt agtagtattc atttggtgga ggtggacgaa
    10741 tttaaaaaat atggaaacgt ctatattact acagaagatg gcactgaagg cgagaaagga
    10801 tttgtaacag atcattcgat attaaaaaag aggaattatg acagaattta ctcttgtgga
    10861 cccactccca tgatgaaagc tgtaggtgct tatgccgaga ataataatat cttttgtgaa
    10921 gtttcacttg aaaatactat gggttgtggc attggagcat gtttatgttg tgtaaccgac
    10981 actactcaag gtcgagtttg tgtttgtaca gagggaacgg tgtttaatat tgaaaaatta
    11041 aaatggtaga tttaaaagtt gacgttggaa aattacattt gaaaaatcct gtaatgactg
    11101 catcaggaac tttcggctat ggaatggagt attcagactt tattgatata tcacgaatag
    11161 gtggtattat tgtaaaagga acaacacttc gcaaacgtga aggaaaccct tatcctcgca
    11221 tgaaagaaac tgcttcaggc atgttaaatg ctgtgggact gcaaaataag ggtgttgatc
    11281 attttattga acatatctat cctcaattaa caaatgtcga tacaaatata attgtgaatg
    11341 tgtcgggttc taccattgaa gaatatgtag aaactgccga aaaattgaat attctcgaaa
    11401 aaatacccgc catcgagctc aatatttcat gtccaaacgt aaaagaaggc ggaatgctct
    11461 ttggaacctc atgtacagca gctaaggagg ttgttgctaa agtgcgaagg gcatataaaa
    11521 acgtattaat tgttaagctt tcgccaaatg ttaccaacat ttccgaaatt gcgcaagccg
    11581 tcgaagaagc cggtgcagac gctatttcgc taattaatac ggtgctcggt atggctattg
    11641 atgccgaaac tcgaaaacct tgcctttcaa cggttacggg gggattatcc ggacctgcca
    11701 tcaagccatt ggcattgaga atggtttggg aaacagctcg agacgttaaa attccagtta
    11761 ttggcatagg tggtatcatg aatgctgccg atgccataga atttatgctt gccggagctt
    11821 cagccgttca agttggaaca gcaaatttta ttgatcctac ggtaaccata aaaattattg
    11881 aaggaataga agattatctc aaccgtcatg gttatacttc ggtttcagat attgttggag
    11941 cattggaaac ctgaaagttt ttagaacaag cttttttcta attttattga ttcaaaaaat
    12001 tcgtgtaatc gggaatttcc ttcaaaaatt caaattttgt atgctccttc atcacccggc
    12061 aacagtaatg ctccaaaccg ttacttacga taaaatattc aacatttagc cggtaattat
    12121 aaacagcaac ctgatcgaac acactttgag taaggggaac gtggggagct ttaaattcca
    12181 aaatcattag aggttgagct tgcccgtcgt ataaaactgc atcacagcgt tttttcattc
    12241 cattgaaaac caattctttc tcaacagcaa gataggcaga aggaaaatgc ttttcttcca
    12301 aaagaaaacg tatgaagttt tgacgaaccc attcttcagg ggtcaactta acatattttt
    12361 ttctctgact gtcgaaaata taaagggttt cattcacctt ttttattttg aaattgtaag
    12421 aaggaagatt taattgcaac atttttccta attttgtccc gaataaattt tatttataga
    12481 gcagtttaag cttacgaagt tacaaaacaa atgctgaaat tactatgaaa acaaaaaaag
    12541 aaattgtaga aaattggctt cctcgatata ccaagcgacc gctcgaagaa tttactccat
    12601 atatcttgct ggttaatttc aacatttatg tggaactttt tgccggttac taccaagttc
    12661 ccattgtcgg aagagatgga aatatgccca atgcttctgc caatggcatt accataatta
    12721 atttcggatt gggaagcccc aatgcagctt tgattatgga tattttgtcg gccattcaac
    12781 ccaaagcagt attatttttg gggaaatgtg gaggattaag agataaaaac aaggaaggag
    12841 attatatttt gcctattgcg gctattcgtg gagaaggaac ttcaaatgat tatttccctc
    12901 ccgaaattcc ctctttgcct gcattcagct tacaacgctc cgtatcttca aatattcgcg
    12961 acattggaaa agattattgg acaggcactg tttacacgac caacagaaga gtatgggagc
    13021 atgacgagaa atttaaggaa tatcttcgaa tgacgcgagc tatggctatc gatatggaga
    13081 cagccacttt gttttcttcc ggtttttaca acggcattcc taccggtgca ttgctgctgg
    13141 tttcggatat gcctatgtac gaatcgggga ttaaaacggc agaaagcgat aaaagagtaa
    13201 cggccaattt tgtggaagaa catttaagaa tcggcataga atcgcttaca tccattatgc
    13261 acaacagcag aaccattaag catttaagat ttgaataaca attagttgcc attataaaac
    13321 tgataaaata tttttgttaa agtgataaaa caggaagccg cagctgatga aattttgcag
    13381 gaaataaaat cgaaaaattt tcattccgtt tattttttga tgggtgaaga accgtattat
    13441 atcgacaaaa tttcaaatta catacaggag aatgtgcttt cagaaagtga aaaggacttc
    13501 gatttaacga ttctctatgg aaaagatgtt gaaatttcga cggtcatcaa tacggcgaaa
    13561 cgatttccca tgattgctcc ctatcaggta gtaatagtga aggaagcgca aatgataatt
    13621 tcatgggaga atttggtata ttatcttcaa aaaccattaa aatcgactat tttggttttt
    13681 tgttacaaat atggaacacc cgataaacga accaagtggt ttcaggaaac aaagaaagca
    13741 ggagttgttt ttgaatcggg aaaactgtat gattatcagg taggagaatg gatacgatcg
    13801 tatcttaaaa ctcgtaaaac gggaataacc cagcaagctg aaaatatgct ggttgattat
    13861 ttgggaacag atttgacaaa aattgccaat gagctggata agttactttt aactaagcca
    13921 caagataccc aacaaataac tccggaatta attgaaagga atattggcat cagcaaggat
    13981 tataatgttt tcgaacttca aaaagcattt gtcaatcgag atattttaaa agccaaccga
    14041 attattctat attttgccga aaataaaaag aataatccgt tggtacttgt attggcgcag
    14101 cttttcaatt tttttgggaa tttgcttttg ttccattaca tgaaagataa aaccgaaaaa
    14161 aatgttgcct ccgaactgaa aatcaatcct ttttttgtaa aagattacca gaacgcagct
    14221 aaaatttatg atagagaaaa gacaataaaa gtactttcgc taatcagaga aacagatgca
    14281 agaagcaaag gggtcgacaa cgccggaatt gatgaaggag aactattaaa agaactggtt
    14341 tacaaaattt tgcactaagc aagtttataa cattattttt ttctattggc aacaggcttg
    14401 tttttatttg catttacatt ggatgttgac gaaatttttt gttttcttgc caccagccaa
    14461 atgccccaaa cgataaaggg aatgcttaaa atttgcccca tattcaataa catacccgat
    14521 tcaaaagctt cctgatcaag cttaataaat tcgagtaaaa aacgtgttcc gaatatacct
    14581 atcaaaaaaa cgccaaaaat aaatccggtt cttttatagg cttctttttt ccagtacaac
    14641 caccaaatta cgccaaatgt tatcaggcaa taaagcatct cataaatttg agttggatgc
    14701 atgggctgcg tttcgcccgc tcgaacaaaa ataaaacccc acggaagatt ggtcggattt
    14761 ccataaattt ccgaattcat caaattcccg agccttatgc acgcaccggt aatggcaaca
    14821 ccaatagcta aacggtcaaa tgcccaaaga tatccttttt tggtaacatt tttattgtaa
    14881 aaatacatgg ctatcagaat gccaattgct ccgccatgac ttgccaaacc cccatgccac
    14941 acatatagta tttcccaagg atgcgacaaa taataatttc cgtatttgaa agtaatacct
    15001 aaaaattgta ccggttcggg gagtaatttc cattcataaa acagacagtg ccccaaacgt
    15061 gccccaacaa taccgccaat tactgcataa ataaaaattt tgtcgagcca aacttctgga
    15121 agcttttcat gtttaaaaat tttctgacct atcagtaaag ccagcaaaat acccacagcc
    15181 caaagtaatc cgtaccatcg tacagacaat gaacctaaac gaaatatttc cggattaacg
    15241 ttccaaacta tataattgag catgccgatt cgattctttt atgggattaa atttttaagc
    15301 atttgctccg tgacagtgtt tgtatttttt tccactaccg cacggacatg gatcatttct
    15361 tcccactttc ttttccacat ggacaggttg cggacgctgt ttttctctgg tatcgccggc
    15421 atctgaattt cctctttcag caatttcgtc cttttgcgtt ttatatttgc tgaaatcctg
    15481 acgtgcaggt atttttgcct ctctgacttt ttccggttct cttactggaa tttgtccacg
    15541 catcaaaatg gagagaacct tgtggttgat attttcaatc atttctttga acaatccgaa
    15601 cgattcgagt ttatatatca acagaggatc tttttgttcg taagttgcgt tttgaaccga
    15661 atttcgcagt tcgtccaact gacgcagatg ttctttccat tcgtcgtcaa tcacataaag
    15721 taaagtctgt ttttcaaaag atttgattac ttctttgcct tctgttttgt aagctgtctc
    15781 caaatgaaca acaacattaa aaattctttt tccatcagtt acaggaatca ggatgttttc
    15841 atatttatct ccttgttgtt catatacctg tttaatcacg gggtaggcta tggaagacaa
    15901 tttttccatt tttcgcttga aggtagtaag tgctgcgtta accactttgt cagtcaaatc
    15961 gcgtgttcgg gcagaattga attcttcttc ggagaaagga acatccatag caaaaaccgt
    16021 caacagttgt tcttcaagtt cttcatagaa tccgttgctt ttggcattag ccaccatatt
    16081 ttcaatggca tcataaatca tgttggtgat atccgattct attctttctc ccattaaagc
    16141 atgtcgacga cgggtataaa taacttcgcg ctgagaattc atcacatcat cgtattcgag
    16201 taatcgttta cggatgccaa agttattttc ttctactttc ttttgtgctc tttcgatgga
    16261 acgggaaatc atagggtgct gaatcatttc tccttcctga aagccaagtc ggtccatgac
    16321 gcccgaaata cgttcagaac caaacagacg catcaaatca tcttccaacg aaacgtaaaa
    16381 aacggaagag cccggatctc cttgacgacc ggcacgtcct ctcaactgac gatcgacacg
    16441 acggctttca tgtcgttccg taccaacgat ggccaatcct ccggcttccc taacttcagg
    16501 agtaagctta atatccgtac cacgacctgc catattggta gcaatggtta ttgttccctt
    16561 gcgtccggct tcagcaacaa tttcggcttc tctttgatgc tgtttggcat ttaacacatt
    16621 gtgttttatt tttctaccgg taagcattcg gcttaataat tcggatactt ctacagatgt
    16681 tgtaccgacc aaaaccggtc taccggcttc catcagccga acaatttctt ctattacagc
    16741 agcatatttt tcacgttttg tcttgtaaac gcggtcttcc atatcttcgc gaataacagg
    16801 gcgattggta ggaataacca ccacatcaag tttataaatg tcccaaaatt caccggcttc
    16861 agtttcggca gttcctgtca taccggcaag tttgtgatac attctgaagt aattttgcaa
    16921 tgtaatggtg gcaaaagtct gtgtggcagc ttctacggta acgcgttctt tggcttcgag
    16981 tgcctgatgc aatccgtcag aatatcgccg tccttccatg atacgtccgg tttgttcatc
    17041 aactatttta acttcgttgt tgataaccac atattcaaca tctttttcaa ataaagtata
    17101 agctttcagt aattgattga cggtgtgaat acgttcggat ttaatggaat agttttgcag
    17161 aatttcatct ttttgtgcct gtttttcttc gggtgtcagc gtatgatttt tttccagttc
    17221 ggcaatttcg cttccgacat ccggtaaaac gaaaaatgtg ggatcttcag aagtttccga
    17281 aagtaaatca aatcctttat cggtaagttc aatagaattg tttttttcat caattacaaa
    17341 atacagggga tcggtagcta tgtgcatatt ccgattgttt tcctgcatat agtattcttc
    17401 cgttttcagc aaaatggttt tatttcccgg ttcactcaaa aatttaatca aagccttatt
    17461 ttttggcaaa cctttgtagg agcggaaaag agccaaagca ccttcttcac gttccttttc
    17521 attttccgat ttcagcaagt tacgggcatc ggtaagatac ttggtagcca acactttttg
    17581 tgcatttacc aaacgctcta cagtagggcg aaattcttcg aaaagctgat cttctccttt
    17641 gggtacgggg ccggaaataa tcaaaggtgt tcttgcatca tcaatcaata cagagtccac
    17701 ttcgtcgaca atggcataat ggtgtttacg ttgtaccaaa tccaaagggc tcgttgccat
    17761 gttatcgcgc aagtaatcaa acccgaattc attattggtt ccgtaggtta tgtcagcgag
    17821 gtaagcctga cggcgttctt cggaatttgg acgatattta tcgatacagt ctacagataa
    17881 tccatgaaac ataaatagtg gccccatcca ttcggagtcg cgtttggaca aataatcgtt
    17941 caccgtaacg atatgaacac cattaccggt cagagcattt aaaaaaacgg gcaaagtagc
    18001 taccagtgtt tttccctcac ctgtggccat ttcggcaatt tttccctgat gcaataccac
    18061 gccaccaata agctgaacgt cgtagtgaac catgtcccat ttaatcatgc tgccacccgc
    18121 caaccattca tttttgtaat gggcttcttc accttcaatg taaacaaaat catgtttggc
    18181 tgcaagattg cgatcaaaat cgttggcttt tacaataatt tcatcgtttt cggcaaaacg
    18241 gcgagcagtt tctttcatta tattgaaagc ttccggaaga atttcgttga gaattttttc
    18301 ctgcttgtcg gttatttcct tttcgagctt atctatttcg gtataaattt tttctcgaag
    18361 gtcgatttcc gtttcttcta tgctgttttt taattcttga atccgattaa cttccggagc
    18421 gacgtattct tgaatacgtt ctttcaagga agctgttctt gctctgagat cgtcatttga
    18481 aagagatttg atttcaggat aaacagcttg aattttttca acgatgggct caatttctct
    18541 taagtcacgt tgtgctttgt ttcccagtat ttttttaaaa aaattattta atgccattat
    18601 tgtaagtttt ttatacgctc tctgatgaat aaataaaatg actcgtgctt aaacatatct
    18661 gtaagcaaaa agttttgcaa agatacattt ttttaaagtt aaaaccagat ggataatgta
    18721 taaaaattct tcttatagtt gataaaaagc aaaaaacgct tccgttgtga gaagcgttta
    18781 aataaatata taaaaggata tttggtttta agtattcatg ccaccacaaa catgaataac
    18841 ctgtccacta acataactcg acaagtcaga tgcaaggaaa agtgtaactt tggcaacttc
    18901 ttcgggagtt ccaccacgac gtaaaggaat attttctacc cacttggcac gctgctcttc
    18961 agtcaaggca tgagtcattt ctgtttcgat gaatcccgga gcaatggcat tggcacgaat
    19021 accgcgtgag ccaaattctt ttgcgaccga ttttgtcaat cctattaatc cggctttgga
    19081 agcagaataa ttggcttgtc ctgcatttcc tgaaacgcct actacagaac tcatgttgat
    19141 aatactgcca tttctttgtt tcatcattat gggagcgcag gcgtgaataa aattaaaagc
    19201 agatttaaga tttacattaa taaccgaatc ccattgagat tctgtcatgc gaatcaataa
    19261 accgtctctg gtgataccgg cgttattgac caaaatgtcg atatggccaa attctttttg
    19321 aatttcttct acaacagcat gcgtttcttc aaaattggca gcattggacg cataagcttt
    19381 cacttttaca ccaagagatt caatttcttt ttgcgtattt gacgcatttt cgtctataac
    19441 caaatcggta aaagcaatat ctgctccctc ttcagccaat ttgatggcaa tggatttgcc
    19501 tattccgcgt gcagctccgg ttacgatagc tacttttcct tctaataatt tcataaaaaa
    19561 ttgtttttga tataattaat tttgtttttt cgagtattgt aaaatttatt aacgattctt
    19621 gaagcgattg ttcaaaacga aaattatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19741 nnnnnnnagt attgtaaaat ttattaacga ttcttgaagc gattgttcaa aacgaaaatt
    19801 atgaaaccca catgtgttac atacacaaac acaaatgaat aaaatattca gacatgtggg
    19861 ttccacaaga cctttaaaaa aataaattat attctgatga aatatgatta tataaaatta
    19921 tcttgcattg atcaacttca atgcttttga aattttacgg tttgatgaaa actgctgaat
    19981 gtttttcttc atttttatag ctttcacgtt tgaagctact gctgatgaat tttctgtctc
    20041 atcataaaaa ataattgttc ctgaatattc accggctata tgatttccaa aataatccaa
    20101 gaagttgtag gtaattgtat tagaattttc ttcattgctg aaagttgcaa ttcccgaata
    20161 aattggataa actgcaacag aattgaaaag aaatgaacca taagaataac catcgtatat
    20221 gaaagccgga agtaaagtta aagggacaat ttgttgtaat gaatcaaaat tttcaatcat
    20281 ttcgtaagtt cctgacggaa ttccattggt agaaagagta tcggtgttga tttcaattaa
    20341 cagagtatct tgcctgttgg taagatataa ggagtaatta ttggaattgc ctgtttgata
    20401 ataatcaccc caaaatgcca attctgcttt tgtaaatgaa gttacgattt catcatctac
    20461 cgtaacattg catgtagccg atttatcgcc ggcagaagcc gtaataatgg catttccctt
    20521 tttttgggcc gatatttctc cgtttttatc tactgaaact actgatgaat cgcttgaact
    20581 ccaagccaaa gatggaacaa tattaccttc gtattgagat tgggcataga gtgtgtcgat
    20641 atcgccaata tgcatagttt tgctggtttc gctaatagtt acagaggtaa cctgattatt
    20701 ttccttttca caagatgtaa acagggaaac gataagaaaa gagacgatac ccaatctaaa
    20761 taatttattc atttttatta caaaattatt ggttatagaa ataagatatt gaattacaag
    20821 ttatgataaa tcaagcattc attgaaacta aaaattcttc gttgtttacg gtacgttcca
    20881 tgcgttcctt caaaaattcc attgcttcca aagagttcat atcggaaaga tggcgacgca
    20941 aaatccacat tctgttcaaa gtatcctgat cgagcagtaa atcttcgcgt cgagtgctgg
    21001 aagccagaat atctacagcg gggaaaattc ttttgttggc caatttccgg tcgagttgca
    21061 attccatatt gccggtacct ttaaattctt caaaaataac atcgtccatt tttgaacctg
    21121 tttcagtaag ggctgtcgca attatggtga ggcttccacc gttttctata tttcttgcag
    21181 ctccaaaaaa tcgttttggc ttgtgaagag cattggcatc cacgccaccg gaaagtattt
    21241 ttccggaagc cggggccact gtattgtaag cacgagccaa acgggtaatg gaatcgagca
    21301 aaatgaccac atcttgtcca cattctacca tgcgttttgc cttttcgtgt accatgcttg
    21361 ccacttttac gtggcgttcg gccggctcgt cgaaagtaga tgatattact tcggctttta
    21421 cgctgcgtgc catgtcagta acttcttctg gtcgctcatc gatgagcaaa acaatcatat
    21481 aaacttcggg atgattggcg gcaatggcat tggcaatgtc tttcaacaaa actgtttttc
    21541 cggttttggg ttgcgcaaca attagtccgc gttgaccttt accaatagga gaaaacaaat
    21601 ctacaatccg tacggaaaga ggatcgttaa cgccggtagt cagtttaaat ttttcgttgg
    21661 ggaaaagagg tgtcaaatgt tcaaaagaaa cacgatcgcg cacatattca ggcgaacgac
    21721 cattgatttt cagaactttt attaaaggaa aatatttttc cccttctttt ggaggacgaa
    21781 tggtgcctgt aaccgtatcg ccggtcttca gtccgaaaag tttgatttga gattgagaaa
    21841 cgtaaatatc atcaggggaa gaaagataat tgtaatcggg cgaacgcaga aatccatagc
    21901 catcctgaat aatttcaagt gttccggttc cttccagaat tccgtcaaaa tcgaattgtt
    21961 tttcggtttt gtcttgtcga ggtgcaattt ttaccttatt ttcattatta ttcccgttag
    22021 catttttgtt gatatttaaa gaaagtccgg gacgtggttc ggaagtttca ataatctgaa
    22081 caggaatcag tgtatctttt tcaatatcaa tatcctgttc ttcggtaatt tcgacttcgg
    22141 gaagattcgg gacttcaaaa ttatcaattt cttccaaagg aatatttact tcaggttttt
    22201 ccgacgtttc agcagaagtc ttttttagca aggttttttg gtttgatttg gaagttttgt
    22261 tgcgtttcga ttgtccgccc gattttttag gttttacttc agtagttgaa gctggagcct
    22321 caatcgtgtt atcagaagtc tgattgttct gtatttcttg tttgggagaa ttttcagtaa
    22381 agggaactaa aggagttgga gaagttgtgg aattggaagg tgcgggtgtg atagttgggt
    22441 ttggtgaaga aaaaactttt tgttcaacat ttttctttac cgaaaccctc atccgttttt
    22501 tcctttcgtt ttcctttttt tcatttgatg caactttttg ctgtgcattt gcaatggctt
    22561 gttgatcaag aatttcataa accagtttgt cttttgtaat ggtaccgggt accttcaggc
    22621 cgagttcgga ggcaatttgt tttaactcgg ataattcttt tgcatttaat tgcaaaatat
    22681 tataattact cataaaattg gataaaaata aagttgggat ttttcaaata ttcaaaataa
    22741 aattcagaat aaaaaaagat tttttgcgta gaaatgcaat aaaaatattt ttgattgtag
    22801 agaatgaaag caggaatgct tttttaaatc gatttgcttt tacgctgcaa aattaatcat
    22861 tttgtgttaa atggacaaat ttattaaaat atttttcaag gataatttgt tgagcgctta
    22921 tttacaacat tttaatttca agaacttcct gcgttgattc ggtaatttta aaccgattgt
    22981 cgagtcgaag acctactacc caaactattt gattttgcga aagcaacagc catgtttcag
    23041 ctttttgctt taaattcagc ttttggtcga taaaaaaatc acttaatttt ttttgtcggt
    23101 tcataccaaa agggaaaaac gtatcaccat tcgtccatct gcgcaaggta agtggatatt
    23161 gaatcaagga cgcatctatt tgtatgattc gattgtcttt tgaaataaca aagttctgtt
    23221 cctttttttt ccttgttatg gaaagttgaa tgggaaagct gatttcggaa tcattttccg
    23281 taatgaaaaa agttgtttca tttttcttta ttttattgct gataatcaaa tattttctgt
    23341 ctttaatcaa ataatgggta ggagaataaa atattttgcc cgattcgtca tgtaaatgct
    23401 tttcaatatc ttgaacaata ctttcgttga agccgtaagg atgtaaaatt tcaaaaagaa
    23461 ttgtttttgg agaagcttgt ttgcacagca aatcaatatt gatttttagc tgatcgttgt
    23521 cgacggtaag cagttgtttt tttatttttt cgatggcatt ttcataaaag gtattgattt
    23581 ccgaaaatcg ttcaatggat tcggaaagaa cttttttcac cgacggattt atttcttcta
    23641 aaagcgggat tacttgaagt cgaattttat ttctttgaaa atcaaccgat tgatttgtag
    23701 aatcttcaat aaaacttaag ttgttgtttt tgatgtattc attaatttca tttctggtgc
    23761 aacagagtaa cggacgaaca atcagaccgt ttttgggaga aattcccgtc aggcctttca
    23821 atccggtgcc acgaacaaga tgcattaata atgtttcaac gttatcatcg gcatggtgag
    23881 caatggctat ggcttgggca ttgatttttt ctgctaatga tgtaaaccac gaataacgca
    23941 attcgcgcgc agccatttct atggaaatat ttttttgttt tgcataatta actgtatcaa
    24001 aatcgaccga ataataagga atatttaagt taaacgataa ctgttgcaca aatttttcgt
    24061 ctctatcgga ttcttctccg cgcaaatgaa aattacaatg agcaacggtg caccgataac
    24121 ccaatttaat taaaatatcg agtaatgcta ctgaatctct accaccactc acaccaacta
    24181 aaatttttcc tttttcgggg aaaagtaatt tatttttttc gatatataat tctacttttt
    24241 tgagtatcat ttccttttat ttacattatc tttgccaaat atagaaactt ataaaaattt
    24301 tttctcgaat tcaaagattt acaaagatat ataatttcca gcaaatagtt tttttcgtgt
    24361 cattgcaaca aaagtgccga tcaatttaaa aaaatatgta ttatggaatc atttttagat
    24421 ttggttaaaa ggcgtcagag tgctcattct tttaccaaca agccggttga gaaagaaaaa
    24481 atcgaccgga ttcttgaaat agtgcgcctt tctccatcgg cttgcaatgc gcagccgtgg
    24541 aaatttattg tagtaacaga cgaagaaaaa cgacaaaaaa ttgctgatgc cacttcaaaa
    24601 agatttcttc ctgtcaatca tttttccaaa caagctccgg ttcttattgt aattgttgaa
    24661 gaaagcacca atttttttac gaaggttggc tgttatgggg taaatcgtca atttccactt
    24721 atagatattg gcattgccac tgctcattta tgtctggctg caacgaatga aggtttggat
    24781 acgcatgtga taggttggta taatgaaaaa aaaattcagc aagctcttga aattcctgaa
    24841 aataaaaaag ttatgctggt tgttgctttg ggatattccg atgaagaagt gcgccccaaa
    24901 aaaagaaaga aaatagaaga attggtttcc tataataagt attgattgaa aattcttatt
    24961 tttttaaaaa tctaaataaa ataagaatat ttatttgtaa attaatgaat tacgaacaaa
    25021 cattagatta tctttattcg cgtttacctg tatttcatca aattggcagt gctgcatata
    25081 aaccgggatt agaaaataca atccgtattc ttacagccct gcatcaaccg caaaattcgt
    25141 ttcgttctat tcatgttgcc ggaacgaacg gaaaaggttc cgtatcacat tttttgtctt
    25201 ccattttgca gtcttcaggt tataaaaccg gcctgtacac ttcgcctcat ttggttgatt
    25261 ttggcgaaag aattcgtgtg aacggcgaaa tgattgataa acaattcgtt atagattttg
    25321 tggaaaatca taaaaaattg attgaagaag tgcagccttc gttttttgaa attactatgg
    25381 caatggcttt cgattatttt gccagcagca aagttgatgt tgcagtaata gaagttggtt
    25441 tgggaggcag gctcgacagt acgaacatta ttcagcctga gctttcggta attaccaata
    25501 tcagttttga tcacgaagaa tttttaggac atactttgca ggaaattgct acagaaaaag
    25561 ccggcatcat aaaaccgcat acaccggtgg ttattggaga agctttgccg gaaacgaaac
    25621 ccgttttttt acagaaagca caggaaatga atgctcccat ctttttttca gaaaacatca
    25681 aacagatttt ttttgatcga tatgaagaaa atcgaatgat agtgaaaact tcagatgaaa
    25741 aatcttatgc ggttggatta acaggcaact atcaacttaa aaatattgca acggttttga
    25801 ctgctgttga tcagctgcgc aaagctaatt ttgaaattcc ggaagcctct ttacaagaag
    25861 gtttggaaaa agttatcgaa aaaacaggac ttcagggaag atggcaaatt atttctgcat
    25921 cacctaaaat tattgccgat acaggccaca atcccggtgg aattgccttt gttgccgaac
    25981 agttaaaaac gcaacattat caaacactaa gaataatttt cggaatggca aacgataaag
    26041 acatcgatac ggtactttca ttgctgccga aaaacggagt ctattatttc acgcaagcca
    26101 atattaaaag ggcatttcct tccgaccaat tattgaaaaa aggacaacaa gcaggacttt
    26161 atggaaaatc tttttcaaat atcgaagaag ccataaagac cgcattggct gacgcaaata
    26221 aagaagattt aatttttatc ggcggcagca attatgttgt tggagaagct ttaacgtttt
    26281 tcgagaagca cgaagaagtg acttaataaa aattcaaaat cagttattcg atcattttca
    26341 ggcagtaata attttataat tgcactgaat tttacctttt gaaatattgg taagttttga
    26401 tttgagcaac tgacgtcgga gtggatttac tcggtcggtg aacaggttgc cttccaaatg
    26461 gtcgtattcg tgttgaataa cgcgtgcttt ataatcttca aaaatctctt cgtattcgtt
    26521 gaagtcggta tcgaaataat ggacttttat cgttttggaa cgaaataccg tttcgtgaat
    26581 cccgggaatg cttaaacaac cttcttcgcc cggcaacaat tcagcactgc gttcgagaat
    26641 agtgggatta atcatggcaa ttttaaaact gcccaattct gcatcttcat ctttaaatgg
    26701 agccaaatca ataaccaaaa ctcttatggg taaaccaact tgtggcgctg ccaacccgac
    26761 accgtcagca tgatacatgg tttcaaacat gttgtttacc aaagatttta attcgggata
    26821 tgaagcatct attggttgtg caacttttcg taaaacggga tttccgtatg tgtaaatagg
    26881 aagaatcatt atttgttttt tattgtaatt cgagtgttat atgtttcttt catttttgta
    26941 tctaactgat tccaaataac tttgcaaaat aattgttgcg gaaattttat cgaccaacgc
    27001 tttgttttgt ctgtcctttt tctttaaacc gccttcgagc atggttttgt gagccaaaac
    27061 cgatgtaaaa cgttcatctt cgtaaataag ttcaatttga ggaaatttct gaaccaattt
    27121 tgcaacaaat ggccggataa atttcatgga atcagattct ttgccgttca tttgtcgtgg
    27181 aaaacctaca acaatggttt caacattttc ttgcaccaaa tatttttcaa gaaaaggaaa
    27241 aacttcattt accggcaaag ttgtcagtcc atttgctgaa atatgcatca catcgcttac
    27301 tgcaattcca actctttttt gcccataatc tattgccagt attcttccca ttttcttcat
    27361 ttgaaaatgc aaagttacaa aatagtgttc attttaaaaa ttttcaactt actcacaacc
    27421 tatggctaaa attattttta attgtcgtat aaaatgaatt tttgtgtcta ttggtttagg
    27481 tacacggatt ttttgtattt ttgttgaatg ataaatataa ttttcttttc gtttgtagct
    27541 ttaaaaataa aattattatt aatttattaa ttatcagcat aataaatgta taggactcat
    27601 aactgtggtg aactccgcat atctcatgtt ggacaaatta ttactttagc cggctgggtg
    27661 cagcgaatcc gtaaaatggg agggatggta tttattgatc ttcgcgatcg ctatggtata
    27721 actcaattgg ttttcaatca ggaaattaat tccgatttgt gggaaaaagc caacaagctg
    27781 ggtcgcgaat ttgtggtaca ggttaccggt aaggtttccg aaagaagcaa taaaaatccc
    27841 aatttaagta cgggcgatat agaaattttg gtttccgaac tcaccgtttt gaatgaatca
    27901 aaaactcctc cttttactat tgaagatgct acggatggtg gcgatgattt gcgaatgaag
    27961 tatcggtatc ttgatttgcg gagaaatgtc gttagaaaaa atttggaact tcgacatcaa
    28021 atgaccattg aaacacgtca atatttggac aatttaggtt ttctcgaagt ggaaactcct
    28081 gtgctgattg cttctacacc tgaaggtgca cgcgatttcg tggttccatc ccgaatgaat
    28141 cccggagagt tttatgcgct acctcaatct ccccaattgt tcaagcaact tttaatggtt
    28201 ggcggattcg atcgctattt ccaaattgtc aaatgtttcc gtgatgaaga tttgcgtgcc
    28261 gatcgtcaac ccgaatttac acagattgat tgtgaaatgt cttttgtgga acaggaagat
    28321 gtgctccaaa cttttgaagg attggtaaag catttgttca aaaccattaa aaatatagat
    28381 tttccagagc catttccacg tatggaatgg agcgaagcaa tgaaacgttt tggttccgat
    28441 aaaccggata ttcgctttgg aatggaactt gtcgaactta aagaaatggt aacgggacac
    28501 gatttttcag ttttcgacaa tgccgaatat gttggtggaa tatgtgctcc ggcttgcgct
    28561 tcctatacac gcaaacagat tgatgagttg acggaatttg taaaacgacc tcaaatagga
    28621 gcaaagggac tgatttatat tcgctttgaa aatgacggtt ccttcaaatc ttctatcgac
    28681 aagttctttt cagttgatga tttgaaaaaa attgctacgt tattttccgc caatccgggc
    28741 gatttaattc ttatcatgtc cggagaaaaa ttaaaaactc aaaaagcact ttgcgaacta
    28801 cgattggaaa tgggaggaag gttaggcctt cgcaaaaaag acgaatttgc acctctttgg
    28861 gttatcgatt ttccattgtt tgaatggaat gaggaattaa atcgctatca agctatgcat
    28921 cacccattta catctccaaa acctgaagat atccctttgt tggataccca acccggtgaa
    28981 gttagagcca atgcctacga tatggtagtg aatggcgttg aattgggtgg gggctctatc
    29041 cgaattcata acagcaattt gcagaataaa atgtttgaat tactgggatt tacaccggaa
    29101 aaagctcaag ctcaatttgg ttttctactg aatgcctttc agtttggagc gcctccgcat
    29161 ggaggtttgg cttttggcct cgaccgtttt gtttctttat tggccggact tgattcgata
    29221 agggactgta ttgcttttcc aaaaaataat gctggaagag atgtaatgat aaatgctccg
    29281 tcaccactgg atccttcgca actggaagaa ttacatttga tgctcaaact ttaaaaaaca
    29341 tttgccatga atgaaaaaca aaccaaaaat gctgccgaat cgattgttaa ggctttggtt
    29401 gaacttagtt tgggaaaaga accaaatatt ttttccaaat caccatttag aaaattggcc
    29461 gaacataaaa attatacttt aatcagagat gcttatatcg attatttgaa agaattcgat
    29521 ggcaaaattg attcggacga agacatgaag cgtcttttcg atttccgcat aaaaattttg
    29581 aattatttca atgacgataa ataaaaatat ttcagtttta ggatagagta tgaagaaaga
    29641 atttcatcaa ttcttatttg acataatata aattatagga aaattaagca aatttatttt
    29701 attctgaaat agtttacaat agtgtgattt caaataatat tccatactaa caaatatcat
    29761 atcaaaattg cagcaaaaca gagtttttta aatgaatgat caataaaatt ttcttcaaat
    29821 tgtttctaag ttatttgcaa aattattttt tgaaaaatat cgtttgaaac tacttaattg
    29881 attcataaat aaatagaaat taaatagaaa tttcatcaaa atataattta tttattaaag
    29941 gtcttatgga acccacatgt ctgaatatta tatttattcg tgtttgtata tacaatacat
    30001 gtgggtttca taattaattg attttcaata gctcaataaa cgatttttaa taagacatta
    30061 tttcacttct cctctcctat ttttgacatt ctatttaaca tattgatatt cagttaatta
    30121 taataacatt ttaattgaat tttattcaaa ttcgcctttt taataaaaaa aaatgagttt
    30181 tgaattaaaa ataagttata tttgcctttg gaattgcttc tacaacaata aaacgaatta
    30241 attttttaaa acagaaatat tatgaaatac tttgctc
//
LOCUS       DAOSIB010000018        30254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_1719_length_30254_cov_14.196894, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000018 DAOSIB010000000
VERSION     DAOSIB010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 30254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30254
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1719_length_30254_cov_14.196894"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..680
                     /locus_tag="PLD44_02970"
     CDS             300..680
                     /locus_tag="PLD44_02970"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOF98333.1"
                     /translation="MNLNKILAGFIFAGRGIRTAFISERNLKIQTCFVVLVLLFGWLL
                     HIRIVEWLICLLCFGLVIGMELINTSIEKLVDLVSPEYHPLAEKVKDIAAGAVLFDSI
                     IAAIIGLIIFVPKIWEYLLRLISS"
     gene            726..1421
                     /locus_tag="PLD44_02975"
     CDS             726..1421
                     /locus_tag="PLD44_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOF98334.1"
                     /translation="MKTNFIVDFINLMYPNLCLICGENLMKNEDYLCMNCLHQIPKTN
                     YHLLPDNPIEKRFWGKAEVFRATSFFYFQKGSLFQKLIHELKYNGNKEIGESLGKYAG
                     ADLISSADFSTVDMIVPVPLHPKKFAKRGYNQSEWIGKGLSSVMQKPQITNNLIRVKE
                     NTTQTKKSVYERFENTEGIFDLKDGLLFSGKHILLIDDVLTTGSTLEACVHALEKTEN
                     KNIKISIFTLAVA"
     gene            1436..1966
                     /locus_tag="PLD44_02980"
     CDS             1436..1966
                     /locus_tag="PLD44_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOF98335.1"
                     /translation="MKNRIEIVKGDITKLQADAIVNAANRTLLGGGGVDGAIHQAAGH
                     ELLEECKKLGGCNTGEAKITKGYHLPSKYVIHTVGPVWRGGDKNEPELLANCYRNSLL
                     LAKKYQIHSLAFPSISTGIYGYPIEKASVIALQEIAKFLKTEEIPEKVIIVCYSDHDY
                     QIYLQNFNEILTSEKN"
     gene            2154..3110
                     /gene="ftsY"
                     /locus_tag="PLD44_02985"
     CDS             2154..3110
                     /gene="ftsY"
                     /locus_tag="PLD44_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOF98336.1"
                     /translation="MGLINLFNKNKDKNENLEQGLQKTRESVFSKLSRAVIGKSKVDD
                     EVLDKLEEVLISSDVGVETTLRIIERIEKRVARDKYVNTNELNNILKEEIAALLSENN
                     TESFTDFNAELPAKPYVIMVVGVNGVGKTTTIGKLAYQFKQAGKKVYLGAADTFRAAA
                     IEQLVVWGQRAGVPVIKQQMGSDPASVAFDTLSSAKANDADVVIIDTAGRLHNKVNLM
                     NELSKIKNVMKKIIPDAPHEVLLVLDASTGQNAFEQAKQFTKATDVNALAITKLDGTA
                     KGGVVIGISDQFKIPVKYIGLGEGVDNLQLFDKNAFVESLFE"
     gene            3167..4456
                     /gene="rimO"
                     /locus_tag="PLD44_02990"
     CDS             3167..4456
                     /gene="rimO"
                     /locus_tag="PLD44_02990"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005852485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOF98337.1"
                     /translation="MKKTVDVITLGCSKNLVDSELLMRQFEALGYQVRHDPQNPNGEI
                     VIINTCGFIGDAKEESIRTILQFAERRKKNKIDKLLVMGCLSQRYQNELQAEIPEVDK
                     FYGKFNFPEILTDLGQDFRADLRWERTLTTPSHYAYVKISEGCNRTCSYCAIPLITGR
                     HRSRPMEEIINEVQSLTSKGVKEFQLIAQDLSYYGADLYKSLKLPELVERLSDIPGVE
                     WIRLHYAYPAQFPYDLLKVMRERDNVCKYLDIALQHISDKMLKVMRRNFTKQETIELI
                     ETIRKEVPGIHIRTTMLLGHPGETEDDVEELKKFIKQTRFERLGAFAFSNEEGTYAFD
                     NYLDNIPEEEKLKRVDEIMAIQQKISAEINQTKIGKRLKVIIDRQEGENFIGRTEFDS
                     PEVDPEVIISDKVQGIEIGQFYLVEIVDADEYDLYAK"
     gene            4478..4768
                     /locus_tag="PLD44_02995"
     CDS             4478..4768
                     /locus_tag="PLD44_02995"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOF98338.1"
                     /translation="MNYKDFIQKVSTQTALTKEETEKLLETAINVFLEQLTENKSIGI
                     QGFGTFELRKKEERIIVHPATKKRWLMPPKLTVHFKSSNVLKEKLNNQLKNE"
     gene            4761..6050
                     /locus_tag="PLD44_03000"
     CDS             4761..6050
                     /locus_tag="PLD44_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOF98339.1"
                     /translation="MNNEKLSLQEFIDLFAQKANLKNKTAEDFIKNIVLVIEEALLKG
                     ESVKIKNFGVFKPQWIEPRKSVDVNSGKEIILPGSYKIGFAPDENLQNIVNEPFSYLE
                     AVELDIIDSSEKKEEETLEPLKIFSEQADEIKSIIAEIQSMSAHPDVKISESKEKQEK
                     IIPTENEDKKSEIFLEPEINNTDIVENESLKHLNDIENITSENVSEEKQEEKNNEIFD
                     KKSNHQKEDDFLEEFDEDTKSEPIGIPFQAYVPSEEYEFHENTEKKRYSWVWIPVVLM
                     LLLGGAGALYYFYPPVKNFVNLKMTKKSPILTERNLPSPAALTDTIQQQRQPEKVDSL
                     QILFDTPRAYSEFIAKERIRSGSRLARMAKRYYGASEFWVYIYEANRDKIPSPNDVPV
                     GTLIKIPKLDARLIDVSNPRCMEKAKELSNLYLSLSK"
     gene            complement(6075..7409)
                     /gene="argH"
                     /locus_tag="PLD44_03005"
     CDS             complement(6075..7409)
                     /gene="argH"
                     /locus_tag="PLD44_03005"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOF98340.1"
                     /translation="MAKLWEKDLPLNKKIESFTIGKDKELDLFLAKYDVLGSIAHARM
                     LNAVGLLTAEETKLLIAELKKIYCSIENGEFKIEEGVEDVHSEVEFLLTDALGDTGKK
                     IHSGRSRNDQILLDLKLFTRAEIERTVRAVSSLFEVCQAQSERYKDILMPGYTHLQIA
                     MPSSFGLWFGAYAESLADDLQLLLAAWKITNRNPLGSAAGYGSSFPLNRQMTTDLLGF
                     DSMNYNVVYAQMGRGKMERIVSTALAGVAATLSKLAFDACLYNSQNFGFIKLPDEFTT
                     GSSIMPHKKNPDVFELIRAKCNKIQSVPQQILLITNNLPSGYFRDLQIVKEVFIQAFN
                     EMIDCIEMTKLMLEKIEVNVHILDDPLYDYLFSVEEVNQLATNGMPFRDAYKKVASEI
                     QNNSFRPQKNLHHTLEGSIGNLCNDQIKAYKEAILAQFTFEKVHQAEKALIE"
     gene            complement(7457..7882)
                     /locus_tag="PLD44_03010"
     CDS             complement(7457..7882)
                     /locus_tag="PLD44_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOF98341.1"
                     /translation="MVTYESEIKKINSNAENIFNKFVDLQNFNKLINNKLSEDQIKDI
                     EFSEDSCSFSIEGFGRIGFRIIEKEPFKTIKFELRELPVQANAWIQLVEKDENDTRMK
                     LTFKAEIPAMLKIMVDKKLKEGVNLAAEAIANAFNNSNL"
     gene            complement(7899..8537)
                     /gene="pyrE"
                     /locus_tag="PLD44_03015"
     CDS             complement(7899..8537)
                     /gene="pyrE"
                     /locus_tag="PLD44_03015"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOF98342.1"
                     /translation="MKTLEKNIAEKLLKIKAIKLQPNNPFIWASGWKSPIYCDNRKTL
                     SYPSIRNYIKIELTRLIMELFPDVEVIAGVATGAIAQGVLVADALNLPFIYIRPTPKD
                     HGLENLIEGDLRPHQKVVIVEDLVSTGGSSLKAVEAIRNNGSQVLGMISIFNYGFPVA
                     EEKFKQAKVKHISLCNYKAILDQALTTNYITQEDMKTLQEWHKDPSAWGLES"
     gene            complement(8645..9010)
                     /locus_tag="PLD44_03020"
     CDS             complement(8645..9010)
                     /locus_tag="PLD44_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOF98343.1"
                     /translation="MSAHNDIGNAGEERAQAYLREKGYKIRHTNWHAQKFELDIVAEK
                     DGLLIIVEVKTRSTENFEHPEDAVTPKKIRNIVQAAQEYIFQNNWEGESRFDIISVIP
                     SGQTFHIEHIEDAFLPPIN"
     gene            complement(9101..9799)
                     /locus_tag="PLD44_03025"
     CDS             complement(9101..9799)
                     /locus_tag="PLD44_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOF98344.1"
                     /translation="MKSRNVFMIYVMSAAIIFSGCKSMSKTASGALIGSGAGAAVGAG
                     IGALIGKDAKSAGIGAAIGTAVGATTGAIIGNKMDKAAQEAAAIEGAKVETITDINGL
                     PAVKVTFESGILFETNKSTLNAASKDALTKFAQIMIQNPTMDIAIIGHTDNTGSLEVN
                     QKLSQERAQAVANFLKSQGVPASQIKDVIGKNFSEPVADNSTAAGRAANRRVEVYMYA
                     SEQMIKEAQQQSQK"
     gene            complement(9848..9920)
                     /locus_tag="PLD44_03030"
     tRNA            complement(9848..9920)
                     /locus_tag="PLD44_03030"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9885..9887),aa:Lys,seq:ctt)
     gene            10191..11633
                     /locus_tag="PLD44_03035"
     CDS             10191..11633
                     /locus_tag="PLD44_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOF98345.1"
                     /translation="MNELESLFQQFTGEKVLLKEELSASGSHRRYFRLESKHFSIIGV
                     EGTSIEENRAFIEMDKLFHKKGLPVPEFYIQSQDGLYYLQEDLGNTLLFDFIAEGRKT
                     GVFCESEKEMLRKTIRKLPEIQVKGAEELDFSICYPQPEFNERSILWDLNYFKYCFLK
                     ATGIDFQENLLEDDFHRFSKILLHSQSNTFMYRDFQSRNVMIKNDEPYFIDFQGGKKG
                     PLYYDVASFLWQAKAKYSEELREELIKNYLSALAELAPVDEMEFREQLKHFVLFRILQ
                     VLGAYGFRGYYEKKPHFLESIPFAIENLRTILQNEISGYPYLLKVLRQMTELKQFREV
                     SVRKPLTVKIYSFSYKKGIPHDDSGNGGGFVFDCRAVNNPGKYERYAHVTGLDESVKQ
                     FLEEDGEILSLLDNAYKLVDASVKRYMDRGFTNLMVCFGCTGGQHRSVYSAQKMAEHI
                     NEKFGIEVQLIHREQNKEQLFPAKKLSYSK"
     gene            11666..12400
                     /locus_tag="PLD44_03040"
     CDS             11666..12400
                     /locus_tag="PLD44_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOF98346.1"
                     /translation="MKALILAAGLGTRLKPITNHIPKALVTIEGKTLLEHQILKLKSA
                     GFNEIIVNVHHFADKIIDFLHSKNNFDIRIEISDERNCLLDTGGAVRKAAWFFDDGKP
                     FLVHNVDILSNVDLMDMMKFHLSSDSLATLVVNQRKTSRQLLFDKQMQLKGWFNETTG
                     QTKPQEINLQLYQKFAFSGIQILSPKVFQLMEKWNEVFSIIDFYLSNVDKEKISGYTN
                     NEIKVLDVGKIQSLEEASIFLKNIIQ"
     gene            complement(12417..13481)
                     /gene="aroC"
                     /locus_tag="PLD44_03045"
     CDS             complement(12417..13481)
                     /gene="aroC"
                     /locus_tag="PLD44_03045"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007135861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOF98347.1"
                     /translation="MSNTIGKFFTFTSFGESHGKAIGGVIDGCPPGIEIDEAFIQNEL
                     NRRRPGQSNISTPRQEEDKVEFLSGIFEGKSTGAPIAFVIWNENQRSADYNHLKDVYR
                     PSHADFTYQQKYGIRDYRGGGRSSARETAARVAAGAIAKLALRKMGIQINAFTSQVGH
                     IAMSKTYEDVDFSLIETNSIRCPEPETAEKMIAYIQQLKEEGDSIGGIITCVVKGVPI
                     GWGEPIFDKLQSRLAQAMLSINATHGFDYGKGFEGVALRGSQMNDEFVKDGDRITTRT
                     NNSGGIQGGISNGQDIYFRVLFKPVATISKKQNTVDIHAQEVELEARGRHDPCVLPRA
                     IPIVEAMTALTLMDLSIIKI"
     gene            complement(13568..14152)
                     /locus_tag="PLD44_03050"
     CDS             complement(13568..14152)
                     /locus_tag="PLD44_03050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOF98348.1"
                     /translation="MRIEENKSVSAKYDLFVDGENQGELELMERATEERPLSFIYGVG
                     MMLPKFEENLYGLQAGDSFEFTINDEDAYGPYDEEAVIDLDKKIFEVDGKIDENVVFE
                     GNVVPLMDTDGNRFNAQIVTVTDELVTVDLNHPLAGQNLHFKGQVLEVRDASEAELAA
                     LMNNSCSCGGNCSSEEGCDCESDSCEDPNCSCNK"
     gene            complement(14270..16987)
                     /locus_tag="PLD44_03055"
     CDS             complement(14270..16987)
                     /locus_tag="PLD44_03055"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOF98349.1"
                     /translation="MNISFCLQAKNLNPSPSDTLQALPDSVLTTNESLTVSVPRENPN
                     KIDAEIIYSASDSIVFYGNGTGFLHGKGDVKYKNITLQADFIRVKMDSSLIYARGTTD
                     SLGNKIGEPVFSEGEATYYSKELTYNLQSKKGYIRNAVTQQGEGYVISDKTKKLDDNI
                     LLIADGKYTTCDKHDNPDFYLYLTKGKIKPGDYIVTGPAYLVLADVPLPIAVPFGFFP
                     FTDKYSSGILMPNYADELNRGFGLTNGGYYFALSDYADLELLGDIYTKGTWALTANSR
                     YIKKYKYNGNFSFSYRVDVTGEKDLPDYSKATNMNIRWTHSQDQKANPYFTFTSSVNF
                     STSGYNRSNINSYFNPNLNSENTKGSSISFTKRFPSQSNLSLSGSMLINQRTKDSTIS
                     LTLPTLNIAVSRFSPFKRKNAVGNERWYEKIYMSYSGTFANSIDTKESLLLSSSLSKD
                     WRNGMRHSIPVSATFNLFKYINISPSFNYTERWYLRSVDKNWDSDKQKEITDTVSGFN
                     RVYDFNMGVSASTKLYGFFIPIRSIFGDKIDRIRHVFTPTIGFGYAPDFGDQRWGYYD
                     SYKKTTKDPSNPASMIEEEVVYSHYQGSLYGYPGSGKSGNINFSFANNIEMKLRNDND
                     TTGKAPFKKVSLIDNFSVSGGYNLAADSMQWSNFSTNLRIKFSDRYSLNLSGAFDPYM
                     YGLNQYGNPVRINKLRWNYHKFPKFLGTNASYSLALNNDTFKKLFSRGDNKKTDVKNN
                     TSSKENLDNPDDESITTSPEINQLGLSEKSNQEIQTDANGYQKVTIPWNINISYSVRY
                     ANTNVFDKEKMEYKMGFTHNLSFSGDISLTPKWRISSSTSYDFVAKQFTYTSVNVSRD
                     LHCWNMSASFVPFGPFQSYYFRIGVNSSMLRDLKYEKRSGYGSTPITWY"
     gene            17290..18516
                     /locus_tag="PLD44_03060"
     CDS             17290..18516
                     /locus_tag="PLD44_03060"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOF98350.1"
                     /translation="MKIPFQKYLLLPYGFLILFLSIFASSVAAQEKKFVVVIDAGHGG
                     HDPGAMGKISREKDINLAVAKELAKLMENNKELKVVLTRKTDKYLTLQERADIANENH
                     ADLFICIHTNSSKSSSAYGAETYTLGLAKTNANLEVAMRENSVILLEENYQTKYQGFD
                     PTSVDSYIMFEFMQDKYLDKSIEFASLVQQHLDRNGRLDRGVRQAGFWVLHRSACPST
                     LIELGFISNLEEELFLSSEYGQKKMAQAIYTAFLEFKKEHDKRNGKPNSTSVSNQAPS
                     KKSENIVSESQNNYSTPIQQAEIENINTANITNQEKQTENILPSTQQPVFKLQILASK
                     KKLSPQANELKGLKSVDYYIENELYKYTVGAETDYNKIVQLKNNLSAKFPQAFIVAFV
                     NGKKVDVKEALRLTGK"
     gene            18634..19545
                     /locus_tag="PLD44_03065"
     CDS             18634..19545
                     /locus_tag="PLD44_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOF98351.1"
                     /translation="MKKNFFNKEVKIGLMVFVAIFLLYFGLNFLKGIDIFTSTRSYYT
                     TYENLDGLVASSPVFIKGYKVGQVDEIQYDFSKEKPFLVKITIEKKINLPKNTKIQLF
                     DNGLMGGKAIQLIFAPYASSQNFCNNGDTLQSEIKPGLMADLSSNLLPKIESISTQTD
                     SLLRSIRSITEGEKLNNSLTSIEKTTADLAETSAQMKNLMKYQLPSIMSNLNGASSDF
                     KSVTSNLKEINFASTMNNVDQTVSNLNLLTNKINNGEGSLGMLLTDQQFYLNLANTSS
                     SANQLLIDLKNNPKRYVHFSLFGSKTK"
     gene            19956..21338
                     /gene="dnaA"
                     /locus_tag="PLD44_03070"
     CDS             19956..21338
                     /gene="dnaA"
                     /locus_tag="PLD44_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOF98352.1"
                     /translation="MHEQLWERCLKVIRDNINEASYKTWFEPIIPLQYENNRFVVQVP
                     SQFFVEYIEEKYIDLLSRTIYREIGAGTQLEYRVLIDQTNGKGTQIPSTAEQPKLKNS
                     NATQGSKNPYDKSSLPNIDPQLNMKYNFNTLIEGKSNKLARTAGITIGAEPGKTAFNP
                     LFVYGESGVGKTHLANAIGVKAKQMHPDKRVLYLSANTFQIQYTDAVRNNTVNDFVNF
                     YQTIEVLIIDDIQEFSGKTATQNTFFHIFNHLHQSGKQLILTSDRSPLLMAGLEQRLL
                     TRFKWGLSAEIEKPDFELRKEILKNKIYCDGLDIPEDVVNFIAQNVTDNIRDLEGVLI
                     SLLAHSTLTDMPIDLQLAEKVVSRIVNIPKKINTVEKIRDTVCDYFSIPVDAIFTKSR
                     KREVVQARQIAMYLSKQFTKNSLSSIGKAIGERDHATVLHACKIISDLMDTDKKFRSN
                     VKAIEEILRN"
     gene            21485..22045
                     /locus_tag="PLD44_03075"
     CDS             21485..22045
                     /locus_tag="PLD44_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98353.1"
                     /translation="MVFRFTILSDEVDNFLRVIDIDAEATFFDLHNAILDAVNFKKDQ
                     ITSFFLCSDDWEKEQEITLIEMDSSSEYDNLVMDKVKLEELLTDEKQKLMYVFDMIAD
                     RAFFIELSEIIPRKKLEKPVCIKSIGNPPEQTLSENDVNQAPKLNVEENFYGDEDFEL
                     DELDEEGFSDFNFDDDNNLFNDDINF"
     gene            22111..23013
                     /gene="miaA"
                     /locus_tag="PLD44_03080"
     CDS             22111..23013
                     /gene="miaA"
                     /locus_tag="PLD44_03080"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOF98354.1"
                     /translation="MQSTLIVILGPTGVGKTEISLQVAQNLQTPIISADSRQIYKELK
                     IGTAAPTSEQLSTVKHYFIGSHSIFENFSAGQFEQEALALLEKHFATNHIALMVGGSM
                     LYIDAVCKGLDNIPTVDTTTRNFWKKQYENQGLEYIRQELKKLDPVYYQQVDPNNYKR
                     ILHALEICSITGNPFSALRTGVHKKRAFDIVKIGLNRPRHELYERINARVDQMMEEGL
                     LEEAKQFYAFKNLNPLNTVGYKELFEFMDGKITLKQAVDKIKQNTRHYAKRQLTWFNH
                     DKDITWFHPDEREKIIRFLQNRLS"
     gene            23024..24031
                     /gene="murB"
                     /locus_tag="PLD44_03085"
     CDS             23024..24031
                     /gene="murB"
                     /locus_tag="PLD44_03085"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOF98355.1"
                     /translation="MNIKYNYSLLSHNTFGIDVKAEEFIEFYTVEEALDLSKTLKQKK
                     WFVIGQGSNLLFLNDFKGVVLHSGIRQMKKIKEDDQFVYFEVGSGVVWDQFVEFCVKN
                     NWSGIENLSSIPGEVGAAAVQNIGAYGMEVQEAIEKVKAIDTATSQLMEFSKENCQYG
                     YRQSIFKNQYKGRYILTSVVFRLSKKPNFRLHYQHLEEEVRKRGNINLQTIRETIIQI
                     RSQKLPDFRTMGNAGSFFVNPVVSKVHFQRLQSEYPEIPHYYVSETEEKIPAAWLIEQ
                     CGWKGKQVGKVAVYAKQPLVIVNCGGASGIEIKQLAEEIQLSVKNKFDIQLQPEVNYI
                     E"
     gene            complement(24230..25198)
                     /gene="floA"
                     /locus_tag="PLD44_03090"
     CDS             complement(24230..25198)
                     /gene="floA"
                     /locus_tag="PLD44_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213154.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOF98356.1"
                     /translation="MGIEIVLLIVLVVVLLLFFYYVPFLLWLSAKVSGVSISLVQLFL
                     MRIRKVPPHSIVQCLIEAHKADLHEIKRDELEAHYLAGGHIERVVHALVSASKANIDL
                     TFKMATAIDLAGRDVFQAVQMSVNPKVIDTPPVAAVAKDGIQLIAKARVTVRANIKQL
                     VGGAGEETILARVGEGIVSSIGSAESHKAVLENPDSISKLVLKKGLDAGTAFEILSID
                     IADIDIGKNIGAQLQMDQAEADKNIAQAKAEERRAMAVALEQEMRAKSQEMRAKVIEA
                     EAEVPKAMAEALRNGNLGVMDYYRLKNIEADTAMRENIGKPSDKTK"
     gene            complement(25213..25704)
                     /locus_tag="PLD44_03095"
     CDS             complement(25213..25704)
                     /locus_tag="PLD44_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOF98357.1"
                     /translation="MDIAIAIILILVGVFFFLVEFFLVPGISFAGIAGFLFVGAGIYY
                     SYSQLGTTAGNVSIAGSVVLLAVAVWIFLRRKTLERISLKAKIDSSIGTVEELNIHAG
                     DTGIALSRLAPMGKVKVNGQTIEAKSVDEFIDEGTPILVKEVFKTNVLVERISENSNN
                     EHA"
     gene            complement(25752..27143)
                     /locus_tag="PLD44_03100"
     CDS             complement(25752..27143)
                     /locus_tag="PLD44_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOF98358.1"
                     /translation="MRSKISLITVLLFFCVAILGQTAAEADRLFQQNQFQKARDAYKI
                     LLEKRPKDALYNYRYARCCYELKDDENAIKHFELSGNRFPLTNKYLGELYMKTYRFSQ
                     SVAAYEEYLKTINETDKEFLTIQKAKKKAELAEKMIQRVDDIQIIDSVVVNKDNFLEF
                     YKFTNDLGSLSATPVKIGKKKDFKITYTTQRQDRKLFSDTVHGQMDIFTSYKLLDEWS
                     KPESVSDIINTKANENYPFLLLDGVTIYFASDGDNSIGGYDLFVSRFRPETNTYFQPE
                     NIGMPYNSIYNDYMMVIDDIHQVGWFATDRFQPEGKVIIFSFIPNTKKTILQSEDSEY
                     IRKAAQLQLFKKAKNFNSDIIPQTSFADEDSVHKIFFVVNDSVIYTDDNQFKTEETRN
                     LWNELEKLNSQLNQKRIQLEQLRMQYSQQEETQNELAPKILTLEKEISEMEDQLTTKT
                     FELRNKENINLMK"
     gene            complement(27219..29915)
                     /locus_tag="PLD44_03105"
     CDS             complement(27219..29915)
                     /locus_tag="PLD44_03105"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOF98359.1"
                     /translation="MKRKNGRILGIIFSILIVLFAVLMILPFAFQGKIFNAVKLQINK
                     MLTAKVDFQNVQLSFIRHFPNASVSINGLKVIGEGDFAADTLVSADKIEAVVNLKSLL
                     SGKGYDIQKINVVNPHIMAHVLADGRANWNIMKDTAEEKTDTAAMKFHLKLKDVFIQH
                     ANIAYLDEQSNMKVIISNLKHHTSGDLTADSSLLSTQTTIDSISFWMNGIPYLLRANA
                     ELNVDIDANINDMKFAFSKNSSRINAIPFSFEGWVKMLDEGFDMDIKLIANKVDLKSI
                     LSMIPAIYSNSFKDLKTGGEVSLNGFVKGQMKDENYPAFDVNLSINDGWFQYPSLPQS
                     VKNIQLAFQIVNPGGSLDSTLIDLSKFSLTLGSNTFNAAAKIASIISDPDIRAKASGR
                     IDLGMVKSFYPLEPGTNLNGLLDLDLNLNGKMSYLENNLYDKFGFQGKLLANNIFLKT
                     SSLPQDISVNRAQVFFNNRYVNLSDFQLKIGKNDLQANGKLENFLAYALKNKTLKGQL
                     NLQSNYLNVNDFLTETTETKNEKMELLVIPQNIDFTLQAFFKQLLYDKMNFSNIQGKL
                     LVQNGEIKFQNLNLEAFGGTLLANGLYSTANVTKPYADINLNIKNVAFNQIFSQVGSF
                     QKFVPFFEKATGNFSTTLSLNTLLNNDMSLNLSSLKANGKFSTSSVALKDVPALSALA
                     SGLKKPELSNPSLKDLAVNFEISNGKLFTKPFDIKLGDIKMNLGGSMGLDQSLDFKGI
                     VQLPDQLNAGRFSNFNVTIGGTYDKPKVKLDLLDTFNEALEETKAKIKEEVATQVDAA
                     KKEVVEKARKEREAALKEAQVQADKIRLEAKKAGDKLIEEATLKGNELVEKAANPIAK
                     KVAEQSARKLVEEARKQADKLNADAEIQANKIIENASKNTNF"
BASE COUNT         9836 a         5669 c         5410 g         9339 t
ORIGIN      
        1 ccacttactt ttttgtcttg aaacaaaaaa gtaagcaaaa aaattcaaga ctgtgcccac
       61 ttcactcgaa aaacttacgc tcgaaaagct gaaatcgccc aaactcattc cgacgaaata
      121 cgtcggaact caaacaagga cgatttttaa acgcttttct cacttgtttt tcggctcacc
      181 ggacaaggtc aacccgaaga gcttggctta tatgttctgt tttacagata tataaaacaa
      241 atgtttcaat ttttgtcatg tattaaaatt tttgtgaatc agaacacatt tttgttaaca
      301 tgaacctgaa taaaatactt gccggattta tttttgccgg aagaggaatt cgtactgcat
      361 tcatttcgga aagaaatttg aaaattcaaa cctgcttcgt tgtgttggtc ttacttttcg
      421 gatggttgtt gcatattcgg atcgtagaat ggttaatttg tcttttgtgt tttggactgg
      481 tcatcggtat ggaactgata aatacttcca tagaaaaact ggtcgatttg gtttcacccg
      541 aatatcatcc cttggcggaa aaagtcaaag atattgctgc aggcgccgta ttgtttgatt
      601 ctattattgc ggcaattatt ggattgataa tttttgtccc gaaaatttgg gaatatcttc
      661 ttcgcttgat ttcatcttga gtaaagtcta attttcgtaa tgtttttatc cgaaatcaca
      721 gccttatgaa aaccaatttt attgttgact ttatcaacct catgtatccg aatctttgcc
      781 ttatttgtgg agaaaatttg atgaaaaacg aagattatct ttgcatgaat tgcctccatc
      841 aaattcctaa aacaaattat catcttttgc ccgacaatcc tattgaaaaa cgtttctggg
      901 gaaaagccga agtgttccgg gctacttctt tcttctattt tcaaaaagga tcactgtttc
      961 aaaaacttat acacgaactc aaatacaacg ggaacaagga aattggcgaa tcgttgggaa
     1021 aatatgccgg agcagatctg ataagttcag ccgatttttc cacagtagat atgattgtac
     1081 cggtaccatt gcatccaaaa aaatttgcca aaagaggtta caaccaaagc gagtggattg
     1141 gtaaaggttt gtcgagcgtt atgcaaaaac cacaaatcac caataattta attcgcgtaa
     1201 aagaaaatac cacccaaacc aaaaaatcgg tttacgaaag gtttgaaaac actgaaggaa
     1261 tttttgattt gaaagatggc ttgttgtttt cgggaaaaca catattgctg attgatgatg
     1321 tattgactac aggctcaaca ttggaagcct gcgtccatgc actcgaaaaa actgaaaaca
     1381 aaaatatcaa aatcagcatt tttactttag cagttgctta aatttaatca caattatgaa
     1441 aaacagaata gaaatcgtga agggcgatat tacaaaactg caggcagatg ccatagtaaa
     1501 tgccgccaat cgaactttgt tgggtggagg aggtgtggac ggtgccattc atcaagctgc
     1561 cgggcatgaa ctattggaag aatgtaaaaa gcttggagga tgcaataccg gcgaggcaaa
     1621 aataaccaaa ggatatcatc tcccttcaaa atacgttatt cataccgttg gacctgtatg
     1681 gcgaggcgga gataaaaatg aaccggaatt attggctaac tgttatagaa acagtttgct
     1741 tttagcaaaa aaataccaaa ttcactcttt agcttttcct tctattagta caggcattta
     1801 cggctatccc atagaaaaag caagtgtcat tgcgttgcag gaaattgcaa aattcttaaa
     1861 aaccgaagaa atacccgaga aagtcattat tgtttgttat agcgaccatg attatcaaat
     1921 ctatctgcaa aacttcaatg aaatattaac ttccgaaaaa aattgatttt ctcttctctt
     1981 tcactccatt ttccagcctt ttctgacaat aaaaattgct gaacactgca tattctacta
     2041 aattatttgt atccaattgt ttgtatttct gtctttgtgg atatattgtt ccaaaatcgg
     2101 aaaactcgga aaaatgttat aattttgtaa attgaaattt aaaaagcaaa agaatgggac
     2161 ttatcaattt attcaataaa aacaaggata aaaacgaaaa tttggaacaa ggattacaaa
     2221 agaccagaga aagcgttttt tccaaattat cacgggcagt aatcggaaaa tcgaaagtgg
     2281 atgatgaggt tctcgataag ctggaggaag ttcttatcag ttccgacgta ggcgttgaaa
     2341 ccactttgcg aatcattgaa agaatagaga aacgtgtggc tcgtgataaa tatgtgaata
     2401 ccaatgaact aaacaacatt ttgaaagaag aaattgcagc acttctttct gaaaataata
     2461 cggaatcctt taccgatttt aatgccgaat tgcccgcaaa accttatgtg atcatggttg
     2521 tgggcgtaaa tggagtgggg aaaaccacta ctatcggaaa acttgcctat cagtttaagc
     2581 aggctggtaa aaaagtatat ttgggagctg ccgatacttt tcgtgctgct gccattgaac
     2641 agttggtagt ttggggacag cgtgccggcg ttccggttat caagcagcaa atgggttccg
     2701 acccggcttc tgtggctttt gatacacttt cgtcggccaa agccaacgat gccgatgtgg
     2761 tcattatcga cacggcagga cgtttgcaca ataaagtgaa tttaatgaac gagctgtcaa
     2821 aaataaaaaa cgtaatgaaa aaaattattc ccgacgctcc tcatgaagta ttgctggttc
     2881 ttgatgcttc taccggtcaa aatgcttttg aacaggcaaa acagtttaca aaagcaaccg
     2941 atgtcaatgc actggctata acaaaacttg atggcactgc caaaggtggc gttgtaattg
     3001 gaatttccga tcagtttaaa atcccggtga aatacattgg cttgggtgaa ggagtcgaca
     3061 acctgcaatt gtttgataaa aatgcttttg ttgaatcctt gttcgagtga tttttgcgta
     3121 taatccaaac ttttaaaata atgtcgttta ccgattcaat ttcagcatga aaaaaacagt
     3181 tgatgtaatt actttgggtt gttctaaaaa tcttgtcgat tccgaattgc tgatgcggca
     3241 gtttgaagcg ttgggttatc aggttcgcca cgatccccaa aatccaaacg gtgaaattgt
     3301 tattatcaat acttgtggct ttatcggcga tgccaaagaa gaaagcattc gtaccattct
     3361 tcagtttgcc gaaagaagaa aaaaaaataa aatagataaa ttgttggtaa tgggatgttt
     3421 gtctcaacgt tatcaaaatg aattgcaagc cgaaatacct gaagtggaca aattttacgg
     3481 aaaatttaat tttcccgaaa tactgaccga tttgggacag gattttagag ccgatttacg
     3541 ttgggaacgt accctgacaa caccctcgca ctatgcttat gttaaaattt cggaaggatg
     3601 caatcgcact tgttcctact gcgccattcc tttgatcacc ggacgacatc gttcacgtcc
     3661 aatggaagaa atcattaatg aagttcagag tctgacaagc aaaggggtta aagaatttca
     3721 gctgattgca caggatttaa gttattatgg agctgacctc tataaatcgt taaagttacc
     3781 ggaactggtt gaacggctgt ccgatattcc gggagttgaa tggattagac tgcactacgc
     3841 atatcctgca caatttcctt acgatttgct caaagtgatg cgtgaacgcg acaatgtttg
     3901 taaatatctg gatattgcac ttcaacatat cagcgacaaa atgctgaaag taatgcgaag
     3961 aaatttcacc aaacaagaaa ctatagaact gatagaaaca ataagaaagg aagttcccgg
     4021 catccatatt cgtacaacca tgctgttggg acatcccgga gaaacggaag atgatgtgga
     4081 agaattgaaa aaattcatta aacaaacccg attcgaaaga ttgggtgcct ttgctttttc
     4141 caatgaagaa ggaacgtatg cctttgataa ttatctggac aatatacctg aagaggaaaa
     4201 actaaaaaga gttgatgaaa taatggctat tcagcaaaaa atttcagccg aaatcaatca
     4261 aaccaaaatc ggcaaaaggc tgaaagttat cattgaccga caggaaggtg aaaactttat
     4321 cggtcgtaca gaatttgatt cgccggaagt tgatccggaa gtaataattt cggacaaggt
     4381 acagggcata gaaattggcc aattctatct ggtggaaatt gtcgatgccg atgaatatga
     4441 tttgtatgcc aaataatcat ctgaaaaaac taaaaacatg aattacaagg attttattca
     4501 gaaagtttcc actcaaactg ctcttacaaa ggaagagaca gagaaattgc tcgaaaccgc
     4561 aataaatgta tttttagaac agttgacaga aaacaaaagt attggtattc aaggatttgg
     4621 aacttttgag cttcgaaaaa aagaagaacg catcattgta catccggcaa caaaaaagcg
     4681 atggctcatg cctccaaaac tcacggttca ctttaaatca agcaatgtat tgaaagaaaa
     4741 actcaacaac cagctgaaaa atgaataacg aaaaattaag tttgcaggaa tttatcgatt
     4801 tgtttgcaca aaaggcaaat ttaaaaaata aaacagccga agacttcata aaaaatatcg
     4861 ttttggtgat agaagaagct ttgctgaaag gagaatcggt aaaaattaaa aattttggcg
     4921 ttttcaaacc acagtggatc gaaccccgca aaagtgttga tgtcaatagc ggaaaagaaa
     4981 ttattttgcc ggggagctat aaaattggat tcgcacctga cgaaaatttg caaaacattg
     5041 taaatgagcc attttcttac ttggaagcag tagaacttga cattatcgat tcttctgaaa
     5101 aaaaagaaga agaaacactc gaacccttaa aaatatttag cgagcaggct gatgaaatta
     5161 aaagcattat cgctgaaatt cagtccatgt ctgcacatcc ggacgtaaaa atcagcgaaa
     5221 gcaaagaaaa acaggagaaa ataattccaa cagaaaacga ggacaaaaaa tcagaaatat
     5281 tcttagagcc tgaaataaat aacactgata ttgttgaaaa tgaatcacta aaacatctta
     5341 atgacataga gaatatcacc tctgaaaatg tttctgaaga aaagcaggaa gaaaagaaca
     5401 atgaaatttt tgataagaaa agcaatcatc agaaagaaga cgattttttg gaagaatttg
     5461 atgaagatac aaaatcagaa cctatcggaa ttccttttca agcttatgtg ccatcggagg
     5521 aatacgaatt tcatgaaaat actgaaaaaa aacgttattc atgggtttgg attcccgttg
     5581 ttctgatgct tttattgggt ggagcaggtg cattgtatta tttttatcct ccggtgaaga
     5641 attttgtaaa tttaaaaatg acaaaaaagt cgccaatttt gactgaaagg aatctcccct
     5701 ctcccgccgc tctaacggat acgattcaac aacaaagaca gccggaaaaa gtggacagtt
     5761 tgcagatttt attcgataca cctcgagctt attccgaatt tattgctaaa gaacgcattc
     5821 gcagtggaag cagattggct cggatggcca aacgttatta tggagcttcg gaattttggg
     5881 tgtatattta tgaagccaac agagataaaa ttccttctcc caatgatgtt ccggtaggaa
     5941 cgctgataaa aattcccaaa ctggatgcac gattaatcga tgtttccaat ccgcgttgca
     6001 tggaaaaagc caaagaacta agtaatttgt atctttcact ttccaaataa aaaaactgaa
     6061 gaaaattttc tgtattattc aatcaaagct ttttcggcct gatgaacttt ttcgaaggta
     6121 aattgcgcta aaattgcttc cttgtaagct tttatctgat cgttgcacaa attgccaatg
     6181 ctgccttcaa gtgtgtgatg aagatttttt tgtggtcgga aagaattatt ttgaatttcg
     6241 gaagcaactt ttttataagc atctcgaaaa ggcatgccgt ttgttgccag ttggttcact
     6301 tcttccacgc tgaaaagata atcgtaaagt ggatcatcca aaatatgaac attaacttca
     6361 attttttcca gcattagttt cgtcatttcg atacaatcta tcatttcatt gaaagcctgt
     6421 ataaatactt ctttaacaat ttgtaaatca cggaaatatc ctgatggcaa attattggtt
     6481 atcaacaaaa tttgttgagg tactgattga attttattgc attttgctcg aatcagttca
     6541 aacacatccg gatttttttt gtgaggcata atgctggagc cagtcgtaaa ttcatcgggc
     6601 aatttaatga aaccaaagtt ttgagaatta taaagacagg catcgaaagc cagtttggaa
     6661 agagttgcag ccacaccggc caaagccgta gaaacgattc tttccatttt tccacgtccc
     6721 atttgtgcat ataccacgtt gtaattcatg ctgtcgaatc ccaacaaatc ggttgtcatt
     6781 tgtcgattga gcgggaaaga agaaccatag ccggcagctg atcccagcgg attccgattg
     6841 gttatcttcc acgcagccag caacagttgc aggtcatcgg ccaaactttc ggcataagcg
     6901 ccaaaccaca aaccaaaaga cgaaggcatg gcaatttgca aatgggtgta accgggcatt
     6961 aaaatatcct tataacgctc gctttgcgcc tgacaaactt caaaaagtga agaaacagca
     7021 cgaaccgttc tctcaatttc agcacgtgta aacaatttca gatccaacaa aatctgatcg
     7081 ttacgtgaac ggccactgtg aattttcttt cctgtatctc ccaaagcatc tgtcagtaaa
     7141 aattcaactt cggaatgcac atcttccact ccttcttcaa ttttaaattc accgttctca
     7201 atggaacaat aaatcttttt caattcggca atcaacaact tcgtttcttc agcagtgagt
     7261 aaaccaaccg cattgagcat tcgggcatga gctatcgaac ccaaaacatc gtatttcgcc
     7321 agaaataggt ctaattcctt atctttgcca atcgtaaacg actctatttt tttattcaaa
     7381 ggtaaatcct tttcccagag tttagccata gtaatgaagc taaatgtttt gaagttacga
     7441 atataaagta aaattcttac aaatttgaat tgttaaaggc attggcaata gcttcggcag
     7501 ccagattaac tccttctttc agctttttat cgaccattat ttttaacata gcaggtattt
     7561 cagccttgaa tgttaatttc atgcgtgtat cattttcatc tttttctacc agttgaatcc
     7621 acgcattggc ttgaaccgga agttcacgca attcaaattt tatagtttta aagggttctt
     7681 tttcaataat tctaaaacca atcctgccaa atccttctat agaaaaggaa cagctatctt
     7741 ccgaaaattc aatgtctttt atttgatctt cagataattt attgtttatc agtttattga
     7801 aattttgtaa atcgacaaat ttattaaaga tattttctgc atttgaattt atctttttaa
     7861 tttcactttc gtaagttacc ataaattttt tctttcaatt aagattctaa tccccatgca
     7921 gaaggatcct tgtgccattc ctgcaatgtc ttcatatctt cctgagtaat atagttggtg
     7981 gtcagtgcct gatcgagaat ggctttgtaa ttgcacaagg aaatatgctt aactttggcc
     8041 tgtttgaatt tttcctcagc tacagggaat ccgtaattaa aaatggaaat cattcccaat
     8101 acttgcgagc cattgttacg aatagcttcc acagctttca aactgcttcc tcctgtcgaa
     8161 acaaggtctt ctacaatcac tactttctga tgaggacgta aatcaccttc aatcaggttt
     8221 tccaaaccat gatctttcgg tgtggggcgg atataaataa atggcagatt caacgcatcg
     8281 gcaaccagca ctccctgagc gattgcgccg gtagctacgc cggctataac ttccacatcg
     8341 ggaaacaatt ccataatcaa acgtgttaat tcgattttga tataatttct gatggaagga
     8401 tacgaaagcg ttttcctgtt atcgcaataa atcggtgatt tccagcctga agcccaaata
     8461 aaaggattgt tgggctgaag ttttattgcc ttaatcttta aaagtttttc ggcaatgttt
     8521 ttttccaaag ttttcatttt gggatagtta taaaattggt aaacaaaaaa ctgtttaatt
     8581 tttctgcaaa gttaagcaaa gtatttaaga agttaaaaca gaatttctcc aatcgtttga
     8641 gaaattaatt aatcggaggt agaaaagcat cttctatatg ttcgatatgg aaagtttgtc
     8701 cggaaggaat aacagagatg atgtcgaagc gtgattctcc ttcccaattg ttttggaaaa
     8761 tatattcttg agctgcctga acgatatttc tgattttttt gggagtaacg gcatcttcgg
     8821 gatgttcaaa attttccgtt gaacgtgttt taacttcaac aataatcaat aagccatctt
     8881 tttcggctac tatatccagt tcaaattttt gcgcatgcca attggtatgt ctgattttat
     8941 atcccttttc tctcaaataa gcttgcgctc gctcttctcc tgcattacca atatcattat
     9001 gagccgacat atcgaaaaaa ttttgcgaaa atttggaaat tcaaacaaaa aaagccgctc
     9061 caaaatataa gagcagctct tttattagaa aataatgaat ttatttttga ctctgctgct
     9121 gagcttcctt tatcatttgt tcgctggcat acatgtacac ttctacgcga cgattggctg
     9181 ctctgccggc agcagtagaa ttatcggcaa ccggctcaga gaaattttta ccaataacat
     9241 ctttaatttg agaagcagga acaccttgcg atttcaaaaa gttggccaca gcctgtgctc
     9301 tttcctgaga taatttctga ttaacttcca gcgaacctgt gttatcggta tggccaataa
     9361 tggcaatatc catagtgggg ttctgaatca tgatctgagc aaattttgtt aaagcatctt
     9421 tcgaagcagc atttaaagta cttttgttgg tctcaaataa aattcccgat tcaaaagtta
     9481 cctttactgc aggcaatcca ttgatatcgg taatggtttc aaccttagca ccttcaatag
     9541 ctgcagcttc ttgagcagct ttgtccattt tattcccaat aattgcacct gttgtagctc
     9601 caacagcagt tccaattgca gcaccaattc ctgcgctttt agcatcttta cctatcaatg
     9661 cgccaattcc ggcacctacg gcagcacccg caccgcttcc tatcaaagct ccactggcgg
     9721 ttttactcat cgatttgcat cctgaaaaaa taattgctgc actcatcaca taaatcataa
     9781 aaacatttct gcttttcatg tctgtagaat tttaaagatt atttttaata ttttaaacca
     9841 ggaatagtga caccggtggg gatcgaaccc acgacccaca gattaagagt ctgttgctct
     9901 accagctgag ctacggagtc gttttatatt tcaaaaaagt gtaagaataa acttacttct
     9961 ctcctttttg tattgcaaag atagaaagat ttttgaaatt ttttcatttt gaattttaaa
    10021 aaaatattta gtgcaaattt ttcttaatca actaccaaat attcaaatat ttatagaatt
    10081 tgaacaattt acttcgtttt ataatttaac gcgcttaaaa attcaagcaa agatgtatat
    10141 ttctttttat atctttgtgt aacctgcatt aattaaaaaa aattctaaaa atgaatgaat
    10201 tagaatctct tttccaacaa tttaccggag aaaaagttct tctgaaagaa gaattgtcgg
    10261 cttccggatc gcacagacga tattttagac tcgagagcaa gcacttttct attatcggag
    10321 ttgaaggaac ttccattgaa gaaaacaggg cttttataga aatggataaa ttgtttcaca
    10381 aaaaaggatt gccggttccg gagttttaca ttcaaagtca ggatggtttg tattatttgc
    10441 aggaggattt gggtaatacg ctgcttttcg attttatagc agaagggaga aaaacgggtg
    10501 tgttttgtga atcggaaaag gaaatgcttc gtaaaacaat tcgaaaactt cccgaaattc
    10561 aagtaaaagg agcggaagaa ctggatttct ccatttgcta cccccagccg gaatttaatg
    10621 aacgttccat tttatgggat ttaaattatt ttaaatattg ttttctgaaa gcaaccggta
    10681 tcgattttca ggaaaatttg ctggaagacg attttcaccg tttttccaaa atcttgttgc
    10741 actcacagtc caatacattt atgtatcgcg actttcagag ccgaaatgta atgataaaaa
    10801 acgatgaacc ttattttatc gactttcaag gtgggaaaaa agggccattg tattatgatg
    10861 tagcatcgtt cctatggcag gctaaagcca aatatagtga agaattgcgc gaagaactca
    10921 taaaaaatta tctttcggca ttagcagaac tggcacctgt agatgagatg gagtttagag
    10981 aacaattaaa acattttgtt ctttttagaa ttttacaagt gttgggagct tatggttttc
    11041 gaggctatta tgagaaaaaa ccgcattttc tggaaagcat tccctttgcc atagaaaatt
    11101 taagaaccat tcttcaaaat gaaatttcag gttaccctta ccttttgaaa gtgctcagac
    11161 aaatgaccga gttaaagcaa ttccgcgaag tatcggtaag gaagccactg acagttaaaa
    11221 tttacagctt ttcctataaa aaaggcattc ctcatgatga tagcggaaat ggaggcggct
    11281 ttgtatttga ttgcagagca gtcaacaatc cgggcaaata cgaacgatat gctcatgtta
    11341 caggactcga cgaatcggtg aaacaatttt tggaagaaga tggtgaaatt ctatctttgc
    11401 tcgataatgc ctacaagctt gtggatgctt cagtaaaacg ctacatggat cgaggattta
    11461 ccaatttaat ggtttgtttt ggatgtaccg gaggacaaca tcgatcggtt tattcggcac
    11521 aaaaaatggc agaacatatc aacgaaaaat ttggcataga agtacaacta attcatagag
    11581 aacaaaataa agaacaactt ttccctgcaa aaaaattaag ctattccaaa taacgtttta
    11641 tatttcaaaa aatctttaat tctgaatgaa agcattgata ctggcagccg gtctgggtac
    11701 gcgacttaaa ccaattacca atcatattcc taaagcgctg gtaacgatag agggaaaaac
    11761 tttactcgaa catcaaattt tgaaattgaa atcggcaggt tttaacgaaa taatcgtgaa
    11821 tgttcatcat tttgccgata aaattatcga ttttcttcat tcaaagaaca attttgatat
    11881 tcggatcgaa atttcagacg aaagaaattg tctactcgac acaggaggag cggtacgcaa
    11941 agctgcatgg ttttttgatg acggaaaacc ttttttggta cacaatgtcg atattctttc
    12001 caatgtagat ttaatggaca tgatgaaatt tcatctaagc agcgacagct tggcaacatt
    12061 ggtggtaaat caacgtaaaa cctcacgtca attgctcttc gacaaacaaa tgcagctgaa
    12121 agggtggttc aatgaaacga ccggacaaac caaaccgcag gaaataaatt tacagcttta
    12181 tcagaaattt gccttttccg gaattcaaat tttgtcgcca aaagtttttc aactcatgga
    12241 aaaatggaat gaagtttttt ccattatcga cttttatttg agtaatgtgg ataaagaaaa
    12301 aatttcgggc tacaccaata atgaaataaa agtgctggat gtaggaaaaa tacaaagttt
    12361 agaggaagcc agcatttttt tgaaaaatat tattcaataa aagcttcttc gataattcag
    12421 attttaatga tggacaaatc catcaaagta agtgctgtca tggcttctac tatcggaata
    12481 gcacgtggca aaacgcaggg atcgtgtcgt ccgcgagctt ccagttccac ttcctgagca
    12541 tgaatatcta ccgtattttg cttttttgaa atggttgcca ctggtttgaa aaggacacga
    12601 aaatatatat cctgcccgtt acttattcct ccctgaatgc ctccggaatt attggttcgc
    12661 gtagtgattc tgtcgccatc tttcacaaat tcgtcattca tttgcgaacc tctgagcgcc
    12721 acaccttcaa aaccttttcc gtaatcgaaa ccgtgagttg catttatgct taacatggct
    12781 tgtgccaaac gcgattgaag tttgtcgaaa ataggctctc cccagccaat cggaacacct
    12841 ttgactacgc aggtaatgat gccacctata gaatcacctt cttcctttaa ctgttggatg
    12901 taagctatca tcttttcggc agtttccggt tccggacaac gaatagaatt ggtctcgatt
    12961 agtgaaaaat caacatcttc gtaagttttg ctcatggcaa tatgtcctac ttgcgaagta
    13021 aaagcattga tttgaatgcc catttttcgc aaagccaact tggcaattgc gcctgctgct
    13081 actctggctg ccgtttcacg tgcagagctt ctgccacctc ccctataatc gcgaatgcca
    13141 tatttttgct gataagtaaa atcggcatgc gaaggtcgat aaacatcttt cagatgattg
    13201 taatcggcac ttctttgatt ttcattccaa atcacaaaag cgataggcgc tcctgtagat
    13261 tttccttcaa aaattcctga aagaaattca accttatcct cttcttgtct gggcgttgag
    13321 atattggatt gtcccggacg acgacgattc aattcgttct gaatgaacgc ttcatctatc
    13381 tctattcccg gcggacatcc atcaataacg cctccaatgg ctttgccgtg cgattcgcca
    13441 aaagaggtaa atgtaaaaaa cttgccaatg gtgttcgaca taactaaaaa atcgttatta
    13501 ctgaaaaaga aaatccaatg gaaaaggctg tataaccttt tccattgctt aaaattcaaa
    13561 aataaaatta cttattgcag gagcaattgg gatcttcaca actgtcgctt tcacagtcgc
    13621 aaccttcctc agaagagcaa ttaccgccac aactgcacga attgttcatt aaagcggcca
    13681 attcggcttc tgaagcatca cgtacttcca acacctgacc tttgaaatgc agattctgac
    13741 ctgccaacgg atgattcaaa tctaccgtaa caagctcatc ggttaccgta acaatttggg
    13801 cattaaatcg attgccatcc gtatccatta aaggaacaac atttccttca aatactacgt
    13861 tttcatcaat ttttccatcc acttcgaaga tttttttgtc caaatcgata actgcttctt
    13921 catcgtaagg gccatatgca tcttcatcat ttatggtgaa ttcaaaagaa tcgccggctt
    13981 gcaatccgta taaattctct tcaaatttgg gaagcatcat tcccactcca taaataaaac
    14041 tcagcggacg ttcttcggtt gcacgttcca ttaattccaa ttcaccttga ttttctccgt
    14101 caacaaataa atcgtattta gctgacactg atttgttttc ttcaattctc attttttact
    14161 ctgttaaatt tatattcaat taattgtttg tttctaattt ttttgtcctt tattaccaat
    14221 aaactgattt ttagtcaaaa agttcattta atattgaaat tttttattac taataccagg
    14281 taatgggcgt agaaccatac ccgctgcgct tttcatactt caaatcgcgc aacatgctgg
    14341 aattgacacc aattctgaaa taataggact gaaaaggtcc aaaaggaaca aaactggctg
    14401 acatgttcca acaatgcaaa tcgcgtgaaa cattaacact ggtgtaggta aattgttttg
    14461 caacaaaatc ataagaagta ctcgagctaa ttctccattt gggcgtcagc gaaatgtctc
    14521 ccgaaaagct taagttgtgg gtaaatccca ttttgtattc cattttttcc ttgtcgaaaa
    14581 catttgtatt ggcatatcga acagaataac tgatattgat attccacgga atggttactt
    14641 tctggtaacc attagcatca gtctgaattt cctgatttga tttttccgaa agtcccaact
    14701 gatttatttc cggtgaagtc gtaatgcttt catcatcagg attatctaaa ttttccttcg
    14761 atgaagtatt gttcttgaca tctgtttttt tgttgtctcc gcgcgaaaaa agttttttga
    14821 aagtatcgtt gttcaaagcc agactgtaag aggcatttgt tccgagaaat ttgggaaatt
    14881 tatgataatt ccaacgcaat ttattgattc tgaccggatt cccatactga tttaatccat
    14941 acatataagg gtcaaatgct ccactaagat ttaacgaata tctgtcactg aatttaattc
    15001 gaagattggt actaaaattt gaccattgca tggaatcggc tgccagatta tatcctccac
    15061 ttaccgaaaa attatcaatc agactgactt ttttaaaagg tgctttccct gttgtatcat
    15121 tgtcgtttct gagtttcatc tcaatattgt tggcaaatga aaaattgata tttcccgact
    15181 tacctgaacc cggatatccg tacaaacttc cctgatagtg ggaatataca acttcttctt
    15241 caatcatgct ggcaggattg gaaggatctt tggtcgtttt tttgtaggaa tcgtaatatc
    15301 cccaacgctg atcaccaaaa tcgggagcat atccgaaacc gatcgttgga gtaaaaacat
    15361 gccgaatacg gtcaattttg tctccaaaaa tggaacgaat aggaataaaa aagccataaa
    15421 gtttggtaga agccgaaacg cccatattaa aatcgtaaac tctgttaaaa ccgctaaccg
    15481 tatcggttat ttctttttgt ttatcgctat cccaattctt atccacagat cgtaaatacc
    15541 agcgttcagt gtaattgaaa gaaggagaga tgtttatata cttgaataaa ttaaaagttg
    15601 cgctcaccgg aatactgtgc cgcattccgt ttcgccagtc tttggaaagc gaagatgaca
    15661 acagcaaaga ttcttttgta tcaatgctat tggcaaaagt acccgaatag gacatataaa
    15721 ttttttcgta ccatctttca ttaccaacag cattttttcg cttgaatgga gaaaaacggc
    15781 tgacagcaat attaagagtc ggtaatgtaa gactgatggt ggaatctttt gtacgttgat
    15841 ttatcaacat acttcccgac aaacttagat tggattgaga aggaaatctt tttgtaaaag
    15901 atatactgga acctttggtg ttttccgaat tcaaatttgg attaaaatag ctgttgatat
    15961 tacttcggtt gtaaccactg gttgaaaaat taacactgga tgtaaatgta aaatacggat
    16021 ttgccttctg atcctgcgaa tgtgtccaac gaatattcat attggtcgcc ttactgtaat
    16081 cgggtaaatc tttttcgccg gtaacatcca cccgatagct aaaactgaaa ttcccattat
    16141 atttatactt ttttatgtat cgtgaattgg cagtaagagc ccaagttcct ttcgtataaa
    16201 tatctcccaa caattccaag tcggcataat cgctcaaggc aaaataatag cctccattag
    16261 tcaacccgaa accgcgattc agttcatcag catagttggg cattaatata cccgaagaat
    16321 atttgtcggt aaaagggaaa aaaccaaaag gaacagcaat tggtaaagga acatcagcca
    16381 acaccaggta agcaggaccg gtaacaatgt agtctccggg ttttattttt cctttggtca
    16441 aatatagata aaaatcggga ttatcatgct tatcgcaggt agtatatttg ccgtcggcaa
    16501 tcagcaaaat attatcatcc agtttttttg ttttatcgct gataacataa ccttctccct
    16561 gctgggtaac tgcatttcga atatatcctt ttttgctttg taaattgtag gtcagctctt
    16621 tcgaataata agttgcttca ccctcactga aaacaggttc tccaatttta tttcccagcg
    16681 aatcagtcgt tcctctggca taaatcaggc tgctatccat tttaacacga ataaaatcgg
    16741 cctgcaatgt tatgttttta tacttcacat cgcctttccc atgcaaaaaa cctgttccat
    16801 ttccgtaaaa aacaatagaa tcggaagcag aataaatgat ttcggcatca attttgttag
    16861 ggttttctct gggtacagat acggttaagc tttcgtttgt agttaaaacc gaatctggca
    16921 atgcttgaag agtatcggaa ggtgatggat ttaaattctt tgcttgcaga caaaatgaaa
    16981 tattcaaccc tgtaattaag aggaataatt tacatttcag gctattgtga aatttaaaat
    17041 tatggtgcat ccttgggatt aaacattttc aattaagctg caaatttaag gaaaaaaaaa
    17101 gctatctaca gtatctatcc ccttttttat gattaatttc aaatttatcc aatggaattt
    17161 gaaataaaaa agattaattt tgcagaaaat aaaaggattg taaaaacttg gaaaattaat
    17221 gaacgggtta tagttcatca gaagataaaa aaacgattcg atgcggaaaa agacaaaaaa
    17281 ttttttggca tgaaaattcc atttcaaaag tacttattgt tgccttacgg tttcctgata
    17341 ttgtttttat ccattttcgc ttccagtgtt gctgcgcagg aaaaaaaatt tgtagtggta
    17401 atcgatgccg gacatggagg acatgatccg ggagcaatgg gtaaaatttc aagagaaaaa
    17461 gatatcaatc tcgctgttgc caaagaattg gctaaactca tggaaaacaa taaggaattg
    17521 aaagttgtac tcacacgaaa aaccgataaa tatcttaccc tgcaggaacg cgccgacata
    17581 gccaacgaaa atcatgctga tctttttata tgcattcata ccaattcttc gaaaagttct
    17641 tcggcttacg gggcagaaac ttatacactg ggtttggcca aaacaaatgc aaatcttgaa
    17701 gttgccatgc gtgaaaactc agtgattttg ctggaagaaa actatcaaac caaataccag
    17761 ggtttcgacc cgacttcggt tgattcctat ataatgtttg aatttatgca ggacaaatat
    17821 ctggataaaa gtatcgaatt tgcttcgttg gtgcaacaac acctcgatag aaacggacgt
    17881 ttggacagag gcgttcgtca ggccggattt tgggtacttc atcgctctgc ctgtcccagc
    17941 acactgatag aactcggatt tatttccaat ctggaagaag aacttttttt aagttccgaa
    18001 tatggacaaa aaaagatggc tcaagctatc tacaccgcat ttttagagtt caaaaaagaa
    18061 cacgacaagc gaaacggcaa gccaaattct acgtctgttt ccaatcaagc tccttccaaa
    18121 aaatcggaaa acatagtttc agaaagtcaa aacaattatt caactcctat acagcaagca
    18181 gaaattgaaa acatcaatac tgccaacatt acgaatcaag aaaaacaaac agaaaatatt
    18241 ttaccgtcaa cacaacaacc tgttttcaaa ttacaaattc tggcttcaaa aaagaaatta
    18301 tctccccaag cgaatgaact taaaggccta aaatccgttg attattacat tgagaacgaa
    18361 ctgtacaaat acaccgttgg agctgaaacc gattacaata aaatcgttca attaaaaaac
    18421 aatttgtcgg cgaaatttcc ccaagctttt atcgttgctt tcgttaacgg taaaaaagtt
    18481 gatgtcaagg aggctttgcg cttgacaggc aaataacaaa atttctaaaa ctgtggtttc
    18541 tgaaaaactc taagaaaagc tctaaatatc tttttgatag caaaaacaat aaattgttta
    18601 tgaaaaaaaa taaattctga taatattcaa gttatgaaaa aaaatttttt taataaagaa
    18661 gtaaaaatcg gattgatggt ttttgtagcc atttttttgt tgtatttcgg tttaaacttt
    18721 ctgaaaggaa ttgatatttt tacctcaacc cgttcttatt atactactta tgaaaatttg
    18781 gatggtttgg ttgcatcttc tcccgtattt ataaaaggat ataaagttgg acaggttgac
    18841 gaaattcaat acgacttttc aaaggaaaaa ccatttttag tgaaaattac tatcgaaaaa
    18901 aaaatcaatc ttcccaaaaa caccaagatt caattattcg acaacggttt gatgggagga
    18961 aaagccattc agttgatttt tgcgccctac gcttcttctc aaaatttttg caacaacgga
    19021 gatacactcc aatcggaaat caaacccgga ctcatggccg acttgagttc caatttgtta
    19081 ccaaaaattg aaagtatttc tacacaaacc gattcgcttt tgcgttccat tcgttctatt
    19141 accgaaggcg aaaaattaaa caacagcctc acttccattg aaaaaacaac tgccgacctg
    19201 gcagaaactt cggcacaaat gaaaaatctg atgaaatatc agcttccatc tatcatgagt
    19261 aatttgaatg gagcttcttc cgatttcaaa tccgtaactt ccaatctaaa agaaattaat
    19321 tttgcttcaa ctatgaacaa cgttgatcaa actgtctcca atttgaattt gcttaccaat
    19381 aaaatcaata acggagaagg aagtttgggc atgcttctca ccgaccagca attttatctg
    19441 aatttggcaa atacttcctc cagtgccaat caattattga tcgacttgaa aaataatccg
    19501 aaacgttacg tacatttttc actttttggg agcaaaacga aatagaattt atttagaatt
    19561 tctctaaata aaaatttgaa aataattttg aatttatttt tttaacacca ttgaaacact
    19621 tgttaataaa aggtttcaat gtaagaaatc ggaaaaaatt caaattccga tttctttttg
    19681 ttaacacctt tagaatcagg ataattttta aaaatttatt atgcaattaa ctgaatatga
    19741 atttgtttca tcaaagcact gacagttaac caaatgaaaa atctgatttt ttttgtagaa
    19801 aaaaagattt gttcttgtca aaaaaatttt agatttttgc ctaactaaat gcagccgtac
    19861 gtctaagtag aattttcaaa agtaaaattt tgaaaatctt tttttggctg aaaatctcac
    19921 ttgtttactt ttttgaatgg attaaaaaaa tgaagatgca cgaacaacta tgggaacgct
    19981 gccttaaagt tatcagggac aatatcaacg aagcgtccta caaaacttgg tttgaaccta
    20041 ttatcccctt gcagtatgaa aacaatcgtt ttgttgtaca ggtaccaagt caattttttg
    20101 ttgagtatat cgaggaaaaa tacatcgatt tgcttagtag aactatttac agagaaattg
    20161 gagccggtac tcaattggaa tatcgtgtat tgattgatca aaccaatgga aaaggcacac
    20221 aaatccccag tacagctgaa cagccaaaat taaaaaactc aaatgccacg caaggatcaa
    20281 aaaatcctta cgacaaatct tcattgccca atatcgatcc acaattgaac atgaaatata
    20341 atttcaatac tctgatagaa ggaaaaagta ataaactggc tcgtactgcc ggtatcacca
    20401 ttggagcaga accgggaaaa acagctttca atcctctttt tgtttatgga gagtcaggtg
    20461 tgggaaaaac gcatttagcc aatgccattg gggtgaaagc caaacaaatg catcccgata
    20521 aaagggtgct ttatctttcg gccaatactt ttcaaattca gtacaccgat gcggtacgaa
    20581 acaatacggt caacgatttt gtaaattttt accaaaccat cgaagttttg attatcgacg
    20641 atattcagga attttcggga aaaacggcca cccaaaacac ttttttccat attttcaatc
    20701 atctgcatca atcgggcaaa caactgatat tgacttccga ccgttctccg ttactgatgg
    20761 caggattgga acaacgtttg ctgacccgat tcaaatgggg actttcggcc gaaatcgaaa
    20821 aacccgactt tgaacttcgg aaagaaatac taaaaaacaa aatttattgc gatggattgg
    20881 atataccaga agatgtagtg aattttattg cccaaaatgt taccgataat attcgtgatt
    20941 tggaaggtgt tttgatttca ctgctggcac attcaaccct cacagatatg cccatcgacc
    21001 tgcaacttgc cgagaaagtc gtcagtcgta ttgtaaatat ccccaaaaaa ataaacacgg
    21061 tagaaaaaat tcgtgatacg gtttgcgatt atttctccat cccggtagat gccattttta
    21121 ccaaaagtcg taaacgagaa gtggttcagg cgcgtcaaat agccatgtat ctgtccaaac
    21181 agttcactaa aaattctctc tcttcgattg gaaaagccat tggagagcgc gatcacgcaa
    21241 ctgtactcca cgcctgcaaa attattagtg atcttatgga tacggacaaa aaattcagat
    21301 ctaacgttaa ggctattgag gaaatacttc gaaattaatt tatggctatt cgatcaataa
    21361 atatctttta aaagctgttt aattattgtt taaacggctt tttttctgaa aaaatcggga
    21421 gaaaatgctt attagctcca aaaaaattac tatttttacc gtcgaaactt aaacaacaaa
    21481 agctatggtt tttagattta ctatattatc ggacgaagtg gacaatttct taagagtaat
    21541 agatatagat gctgaagcaa ctttctttga cttacataat gccattctgg atgccgtaaa
    21601 cttcaaaaaa gaccagatta cttctttttt cctctgttct gacgactggg aaaaagagca
    21661 ggaaattacc cttattgaga tggattcttc ctcagaatat gataatttgg tgatggataa
    21721 agtaaaattg gaagagttgc ttaccgacga gaaacaaaaa ctcatgtatg tgtttgatat
    21781 gattgctgat agagcatttt ttatagaact aagtgaaatt attccacgaa aaaaacttga
    21841 aaaaccggtt tgcatcaaat caatcggcaa tccacccgaa caaaccttaa gcgaaaatga
    21901 tgtgaatcag gctccaaaac tgaacgtgga agaaaacttt tatggcgacg aagattttga
    21961 actggatgag ttggatgaag aaggatttag cgatttcaat ttcgatgacg acaacaatct
    22021 gttcaatgat gacatcaact tttgatatca attgattttt tcactgacag aaaataacgg
    22081 gtttattttc tgttttttta ttttttacga atgcaatcaa ccttaatagt aattcttggc
    22141 cctaccggtg taggtaaaac cgaaataagc ctgcaagtag cccaaaattt acaaacgccc
    22201 atcatttcgg ccgattcgcg tcaaatatac aaagaactga aaattggtac agctgcacct
    22261 acatccgaac aactttccac agtaaaacat tattttatcg gttctcattc aatttttgaa
    22321 aatttcagtg ccggacaatt tgaacaggaa gctctcgcat tgcttgaaaa acattttgcg
    22381 accaatcaca tcgccctaat ggttggaggt tcaatgcttt acattgatgc tgtatgcaaa
    22441 ggactcgaca acatcccgac agtagatacc actacaagaa acttctggaa aaaacaatat
    22501 gaaaatcagg gacttgaata cattcgacaa gaattgaaaa aattggatcc tgtatattat
    22561 caacaagtgg acccaaataa ttacaaaaga attttgcatg cgctggaaat ctgttccatc
    22621 accggaaatc ctttttcggc attaagaacc ggcgttcaca aaaaaagagc tttcgatatt
    22681 gttaaaatag ggctgaacag acctcgtcat gaattgtatg aacgcatcaa tgcaagagta
    22741 gatcaaatga tggaagaagg cttattggag gaagcaaaac aattttacgc ttttaagaac
    22801 ttaaatcctt tgaacactgt tggatataag gaacttttcg aatttatgga cggtaaaatt
    22861 acactaaaac aagctgtcga taaaattaaa caaaacaccc gacattatgc caaaagacaa
    22921 ttaacatggt tcaatcacga caaagacatt acttggtttc atcccgatga aagagaaaaa
    22981 ataatccgtt tcctacaaaa ccgactttct taacttctca agaatgaata ttaaatacaa
    23041 ctattccctc ctttcgcaca atacttttgg cattgacgta aaagccgaag aatttatcga
    23101 attttacacg gttgaagaag ctttagattt gtcgaaaaca ttaaaacaaa aaaaatggtt
    23161 tgtgattgga cagggaagca atttgctgtt tttgaacgat tttaagggtg tcgttttaca
    23221 ttccggaatt cggcaaatga aaaaaataaa ggaagacgac caatttgtct attttgaagt
    23281 gggctcggga gttgtgtggg atcagtttgt cgaattttgt gtaaaaaata actggagtgg
    23341 cattgaaaat ctctcatcaa ttccgggaga agtgggtgca gcagccgtcc agaatattgg
    23401 cgcttacggt atggaagttc aggaagccat tgaaaaagtg aaagcaattg atactgcaac
    23461 ttctcaattg atggaatttt ccaaagaaaa ttgtcaatac ggttatcgtc aaagtatttt
    23521 taaaaatcaa tacaaaggtc gatacatttt gacttctgtg gtcttccgtc tgtcgaagaa
    23581 acctaatttc aggcttcatt accagcattt ggaagaagaa gtcagaaaaa gaggaaacat
    23641 aaacctgcaa accattaggg aaaccattat acaaataagg agccaaaaat tacccgattt
    23701 cagaacaatg ggcaacgccg gcagtttttt tgtcaatccg gtcgtttcca aagtgcattt
    23761 tcagcgtttg caaagtgaat atcccgaaat acctcattat tatgtatctg aaacagaaga
    23821 aaaaattcct gccgcatggc ttattgaaca atgcggctgg aaaggtaaac aggttggaaa
    23881 agtcgctgtt tatgcaaagc aacctttggt aattgttaat tgtggtggag cttcgggcat
    23941 tgaaattaaa caattggccg aagaaattca actttcagta aaaaacaaat ttgatattca
    24001 acttcaaccc gaagtcaatt acatcgaata atttctttat ataaatatct tattttcaac
    24061 tttcaagaag atttcagtct actttttcac ctatatgcct aaaaaagaaa gtgtcctttt
    24121 gggacaccct cttaaaattt agatttgatc tacttgtttc caatcaaaat aatttttcag
    24181 caaacaaggt tcaattcaga ttcagtaacc attaaattta gaagctactt tatttggtct
    24241 tatcggaggg tttaccgata ttttcacgca tagcagtatc agcttcaata tttttcaatc
    24301 tgtagtagtc catcacaccc agattcccgt ttctcaatgc ttcagccata gctttaggta
    24361 cttcagcttc agcttcaatt acttttgcgc gcatttcctg tgattttgcc cgcatttcct
    24421 gctcaagggc aacagccata gcccgacgtt cttcagcttt agcctgtgca atatttttat
    24481 cggcttcggc ctgatccatt tgcaactggg caccaatatt ttttcctatg tcgatatcgg
    24541 caatgtctat ggataaaatt tcaaatgccg tgccggcatc caaacctttt ttcaatacca
    24601 acttggaaat ggaatccgga ttttccaaaa ctgctttatg cgattcggcc gaaccaatgg
    24661 aggaaacaat tccctctcct actcttgcca gtattgtttc ttctccggct ccacctacca
    24721 actgtttgat attggcccgc accgtaacgc gcgctttagc aatcagctga ataccatctt
    24781 tagcaaccgc agccaccggc ggtgtatcga taacttttgg gttgaccgac atttgaacgg
    24841 cctgaaaaac gtctcttcct gccaaatcga tagcagttgc cattttgaaa gttaagtcaa
    24901 tattggcttt agaagctgat accaaagcat gaaccactct ttcgatgtgt ccgccggcaa
    24961 ggtagtgagc ttccagttca tcgcgcttta tttcgtgcaa atcggctttg tgagcttcta
    25021 tcaagcattg aacaatagag tgaggcggaa cttttctgat gcgcatgagg aaaagctgca
    25081 ccagagaaat actcacaccg gaaactttag ccgagagcca caacaaaaaa gggacatagt
    25141 aaaaaaacaa taataaaaca acgaccagta caataagtaa cacaatttca attcccatca
    25201 taatttataa atttaagcat gttcattatt tgaattttcc gaaatacgtt caaccaacac
    25261 attggttttg aaaacttcct ttaccaaaat tggggttcct tcatcaataa attcatctac
    25321 acttttggct tctatggttt gtccattcac tttgactttt cccattggag caagtcttga
    25381 gagcgcaatt cccgtgtctc ccgcatgaat gttcaattct tcgacagtac caatactgga
    25441 atctattttt gcctttaaac tgattctttc caatgtttta cgtcgcaaaa aaatccaaac
    25501 tgcaacggcc aaaagaacaa cactgcctgc aatagaaaca ttccctgctg ttgttcccaa
    25561 ctgggaatag gaatagtaaa ttccggcacc aacaaacaaa aaaccggcta ttcccgcaaa
    25621 cgaaattccc ggcaccagaa aaaattctac cagaaagaaa aaaacaccaa ccaaaatcag
    25681 aataatagct attgctatgt ccattgtctt aaaatttttg gtaaaaataa aaaataaatt
    25741 tcgaaataca actacttcat taagtttata ttttctttat ttctgagttc aaaggttttt
    25801 gtagttaact gatcttccat ttccgaaatt tccttttcca aagtcagaat ttttggcgcc
    25861 aattcatttt gagtttcttc ctgctgactg tattgcattc tcaattgttc caattgaata
    25921 cgtttttgat ttagttgcga atttaatttc tccagttcat tccacaagtt acgggtctct
    25981 tcggttttaa actgattgtc atccgtgtag attacagaat cgttcactac aaagaaaatt
    26041 ttatgaaccg aatcttcatc agcgaatgat gtttgaggaa taatatccga attgaaattt
    26101 ttggcttttt tgaatagttg taactgtgct gcttttctta tatattctga atcctctgac
    26161 tggagaatgg tttttttagt gttggggata aaggaaaaaa taattacttt tccttccggc
    26221 tgaaagcgat cggtggcaaa ccatcccact tgatgaatgt cgtcaataac catcatatag
    26281 tcattgtaaa tcgaattata aggcattcca atgttttcgg gttgaaaata ggtgttggtt
    26341 tccggacgga agcgggaaac aaacaaatcg tagcctccaa tagaattatc tccgtccgac
    26401 gcaaaatata tggtaacacc gtccaaaagt aaaaaaggat aattttcgtt ggctttggta
    26461 ttgattatat cggaaaccga ttcgggtttt gaccattcat ccaaaagctt gtaagaggta
    26521 aaaatatcca tttgaccgtg aacagtatcg gaaaacaact ttctatcctg acgttgagtg
    26581 gtataagtta ttttaaaatc cttttttttg cctattttca caggtgttgc cgatagcgaa
    26641 cccaagtcgt tagtgaattt gtaaaattcc aaaaaattat ctttattaac caccacacta
    26701 tcaatgattt gaatatcgtc cactctttga atcatttttt ctgccagttc agctttcttt
    26761 tttgcttttt gaattgttaa gaattccttg tcggtttcat ttatggtttt tagatattct
    26821 tcgtaggctg ctaccgattg ggaaaaacgg taagttttca tatacaattc tcccaaatac
    26881 ttgtttgtca aaggaaatcg attacctgaa agttcaaaat gttttatggc attttcatca
    26941 tctttcaatt cgtaacagca acgcgcatac cgataattat acaaagcatc ctttggcctt
    27001 ttctccaata atattttata ggcatcgcgt gctttctgaa actggttttg ctgaaaaagt
    27061 cggtctgctt cggcagcagt ttgacccaaa attgctacac agaaaaacaa caatactgta
    27121 atcaaagaaa tttttgaacg catgacaaat attttcaaaa tatataattt gttgaaatta
    27181 aggtacttaa ttaacaatct tgcataaatg ctttagaatt agaaattcgt atttttcgat
    27241 gcattttcaa taattttatt ggcttgaatt tcggcatctg cattcagttt atcggcttgc
    27301 ttgcgtgctt cttctaccag cttacgagcg gattgttcgg caactttttt ggcaattgga
    27361 ttggctgctt tctcaaccaa ttcgttgcct ttcagggtgg cctcttcaat cagtttatcg
    27421 ccagcttttt tagcttccaa tcttatttta tctgcttgaa cctgtgcttc ttttaaagca
    27481 gcctcacgtt ctttccgagc cttttcgaca acttcttttt tggctgcatc tacttgtgta
    27541 gcaacttctt ctttaatttt agcttttgtt tcctcaagcg cttcgttaaa tgtatccaac
    27601 aaatccaatt tcacttttgg tttatcgtag gttccaccaa ttgttacgtt aaagtttgaa
    27661 aagcgaccgg catttagctg atccggtaat tgaacaattc ctttaaaatc aagtgattga
    27721 tccaaaccca ttgaacctcc caaattcatt ttaatatctc ccaattttat gtcgaatggt
    27781 ttggtaaaca gtttcccgtt tgatatttca aaattcacag caaggtcttt caatgatgga
    27841 ttgcttaatt ctggtttttt caggcctgat gccaatgccg acaatgccgg aacatctttt
    27901 aaagcaactg acgaagttga aaattttccg ttggctttta atgaactcag attcaagctc
    27961 atgtcgttat tcaataaagt attcaacgac aatgtggtag aaaaatttcc ggtagctttt
    28021 tcgaaaaatg gaacaaattt ttgaaatgaa ccaacttgcg aaaaaatttg attaaaagcc
    28081 acatttttaa tgttcagatt tatatccgcg tatggttttg taacgtttgc cgtgctgtaa
    28141 agtccatttg ccaataacgt tcctccaaag gcctccaaat tgagattttg aaatttaatt
    28201 tcgccatttt gtaccaaaag ttttccctga atattggaaa aattcatttt gtcgtaaagc
    28261 aattgtttga aaaatgcctg aagtgtgaaa tcaatatttt gaggaattac caacaattcc
    28321 attttttcat ttttagtttc tgttgtttcg gtgaggaaat cattcacgtt caaataattg
    28381 gattgcaagt tcagctgtcc ttttaaagtt ttgttcttca aagcataggc caggaaattt
    28441 tcaagttttc cgtttgcctg caaatcgttc ttgccgattt tcaattgaaa gtccgataaa
    28501 tttacatatc ggttattgaa aaaaacctga gctcgattaa cagaaatatc ttgtggcaaa
    28561 gagcttgttt tcagaaaaat attgttggcg agcaattttc cctgaaaccc aaatttatcg
    28621 tacaaattat tttccagata cgacattttc ccattcagat tcaaatcgag gtcgagcaag
    28681 ccgttcaaat tcgtgccggg ttccaaagga tagaaggatt ttaccattcc caaatcaatt
    28741 cgccctgaag ctttggcgcg aatatcggga tcggaaatga tggaagcaat tttagctgca
    28801 gcattgaaag tattactccc caacgttaaa gaaaacttgg aaaggtcaat taaagtactg
    28861 tcgagcgaac caccgggatt gacaatttgg aaggcaagct gaatattttt aacggattga
    28921 ggcaatgaag gatattgaaa ccagccatcg tttatcgaca aattgacatc aaaagccgga
    28981 taattttcgt ctttcatctg acctttgaca aaaccattca aactgacttc tccaccggtt
    29041 ttcaaatctt taaaagaatt ggaatagatg gccggaatca tggaaagaat tgattttaaa
    29101 tctactttat tggcaattaa cttaatgtcc atatcgaaac cttcgtccaa catttttacc
    29161 cacccttcaa aagaaaatgg aatagcgttg attcgtgaag aatttttgct aaaagcaaat
    29221 ttcatgtcgt taatattggc gtcgatatct acattcagtt ctgcattggc ccttaaaagg
    29281 tatggaatac cattcatcca aaagctaatg gaatcgatgg ttgtttgtgt agacagaagc
    29341 gagctgtcgg cagttaaatc tccactggta tgatgtttca ggttgctgat aatgactttc
    29401 atgttgctct gttcgtccaa ataagcgata ttggcatgtt gaataaaaac atcttttaac
    29461 tttaaatgaa atttcatggc agcagtatcg gttttttctt ctgccgtatc tttcataatg
    29521 ttccaattag ctcttccatc agcaagtaca tgagccatta tatgcggatt aactacattg
    29581 atcttttgaa tatcgtaacc ttttccggaa agcaaacttt tcaaatttac aactgcctca
    29641 attttatcgg cagaaaccag cgtgtcagca gcaaaatcac cttctccgat aactttcaat
    29701 ccgttaatgc tcaccgaagc atttggaaaa tgtcgaataa aactcaattg aacattttga
    29761 aaatcaactt ttgccgtcaa cattttattg atttgcaatt tcaccgcatt gaaaattttc
    29821 ccctgaaaag caaatggtaa aatcatcagg acagcaaaca aaacaatgag gattgaaaaa
    29881 attattccaa gaattcgtcc atttttcctt ttcatattgc aaaattttta aactgaatca
    29941 aaacaaaggt acaataaact gccgaaaaaa gtaaaacttt tttttggttt ttcaattatt
    30001 tttctcttat tccggaaaac ctctaacttt gcactgaaaa tgaaaagttc tcaatcatga
    30061 gtcccctcca aaaagttact caaagagaat tagtttttta aagacattaa gataaaaaag
    30121 gatacaaaat attcaaaaag ttttttgaaa aatttgcata tattcttata tttcactatg
    30181 ttagaagggt tttggagggg atgatgattt taaaagaagg tcagggctgg agcgacgagc
    30241 agttattttg gcca
//
LOCUS       DAOSIB010000019        29650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_1769_length_29650_cov_10.549789, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000019 DAOSIB010000000
VERSION     DAOSIB010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 29650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29650
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1769_length_29650_cov_10.549789"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1239)
                     /locus_tag="PLD44_03110"
     CDS             complement(37..1239)
                     /locus_tag="PLD44_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HOF98360.1"
                     /translation="MEFNNSLQRKIKQQEILLSQKNEILPSKNNIYNRYAYPIITPEH
                     IPLCWRYDFDKQTNPYLLERIGVNATLNSGAIKWNGKYVLVVRVEGVDRKSYFAIAES
                     NNGVDNFRFREKPLSLEVPEGSSETNVYDMRLTAHEDGWIYGIFCSESKDPNAAPGDL
                     TSAIAKAGIVRTKDMNTWERLPNLVSKSQQRNVVLHPEFVNGKYALYTRPQDSFIDPG
                     SGGGIGWALVDSMENAVITNEKIINHRYYHTIKELKNGEGPHPLKTGKGWLHLAHGVR
                     MCASGLRYVLYLYLTDLQDPSKLIAEPAGYFMAPEDDERVGDVSNVLFSNGWIADADG
                     TVYIYYASSDTRMHVATSTIDKLLDYCLNTPSDGYTSKQSVLTICKLIDKNKTLFTNK
                     SFEFHKTI"
     gene            complement(1414..2547)
                     /locus_tag="PLD44_03115"
     CDS             complement(1414..2547)
                     /locus_tag="PLD44_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOF98361.1"
                     /translation="MKKLTLFFLVMVFAVSSMADDVTAVANWKFPIYNWLQTDSLEAE
                     ADQAKYIRAAIKATQAPANFDAASADFDAVWDVAGTEFALGGIPEKRIAKAGVDNGPA
                     DFTGAFKVIYDDYSIYVLIKCTDEDINNKDVVEIAWAQDLRIQAIADRALAQGAKKSN
                     AYMRFAAFGGLKATFTASAFRDAMMINFDAAGTGSVDWAGTNALLAANLLVDDKGATT
                     GSGVVKRIITIGFPALTGVFRPDFDVNIWKNLNAGKGISFDMKYNDYDAADPKDANNK
                     DQTGSYWWNARTDDAWYLNWYAGFLAPDVPSAVKNPDATKSIFVNKTATKIELSELAN
                     GTIYNLAGKQIKEFRNVTEVNISDLAKGVYIVKANGETMKIVK"
     gene            complement(2629..3864)
                     /locus_tag="PLD44_03120"
     CDS             complement(2629..3864)
                     /locus_tag="PLD44_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98362.1"
                     /translation="MKTRHIFFTILLLVFFTACEDEVIVDPQFSLSFEEQGFDNAFAG
                     TTFYVKVKGSAEFLTLYDGKPGAEWDSIGAKGVDFNLADSLPVTYPTAGTYNLTVVAT
                     TTSDFGKSIQRRTKTVPITVIDIRNEFNSFVMNGVTGTITADKQILFSFPDNITDFNF
                     KPVFTVNSPDAKVYVSGEEQTSGVSVQNFSPGNPVVYTVKSRDGEENIYTVYVSTFPA
                     SNEKQLLSLQLFPNSPNLGYTNSNGEIATIDETNKEINLALNYGTNATSVRLTVSSSP
                     KSTVLLNGIPYSSTRRYNLTTLTGVEVVAENSTKQFYKLILTTQDPVIEFTFEGLVPA
                     PVGVIDKNNKTITIDVLKVTDITKLKAKWTGSVGTVKIGNVTQINGVTENDFSTPLVY
                     TFYKGTSVGDSYTVIVIKK"
     gene            complement(3891..5540)
                     /locus_tag="PLD44_03125"
     CDS             complement(3891..5540)
                     /locus_tag="PLD44_03125"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOF98363.1"
                     /translation="MKKLVILLLSFLIVTACSDDFLDTVNKNNLDNTSFLKNENELIY
                     AINAAYTPFAYYGVFGLNYYKIFNSLDSYIWFEGQSGFDIMNFSPTNADLQEMYLDFY
                     YVLFRTSDILSKMTPLKDKIEENKYKQYQAQLKALRGLSYFYLVTLFDTPVYYDETNV
                     PTDALKSYRNGTREQFWSKLEADLDFAIANLPASWPNQDLGRITSGGASAILGKALLY
                     KHYHYYLRFDKANTDEAKANIEKAKSVLNNIIKSGNYSLSRPAIKTKEHYQAALLCNF
                     SFVDIPVGSTIYKAENNQESVYEIQYNDNPGTNTYLPAYLMGGNMLSLYFSTHQNGYR
                     NMVIDPSLWNTFESVSSHPAGYAKDPRAYATCYLDGDMMDWRPESGYAKPFDGTVNAK
                     GGIFPTVYSGFTLSKAIGIKKYFYPTYNHGSAPAASPVNFRVIRYSDVLLMYAEACFQ
                     SGSDLDEGLKALNEVRARVDMPPVSELTPSAIMHERDVELATEGHRYNDIIRWSYDSR
                     FNINFATMVSDKFNIKRHLYFPIPQYEIDANKGALKQNAGW"
     gene            complement(5578..8766)
                     /locus_tag="PLD44_03130"
     CDS             complement(5578..8766)
                     /locus_tag="PLD44_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOF98364.1"
                     /translation="MKKQVLYFSRISLFIISLIINSQIFAQNITGKIIDGTTNEPMIG
                     ATVQLKGKMVGTVSDLEGNYSIQASPSDILIFSFTGYDKQEIPVGDKKVINVTLKESA
                     TQLAEVVSIGYATVKKSDLTGSVAVVTSKELTKNPASSAAQALQGKAPGVLVSQNGAP
                     GGGATIRVRGVGSINKSADPIFILDGIQVPNINSIQPQDIETFQVLKDASASAIYGAN
                     GSNGVIIITTKRGKSGKPQVNFSSYLSLNMKPIEYDVMNAQQYSDFYSQVLYPEYGLD
                     KTYNNAVNPAHVLSPEFRQKYYGAGWEQGTNWQSEIFKNGFNQNYNLSVAGGGENSNF
                     NISFGYLNEDGTVIKNNYERYQIRANSDFKLSQHIKIGENLSASYSTRETPMTVQSSI
                     YDLNTSPLMRIYNPELLGGYESYQMQYWYDINGNLTSTPQTGNGYLNTLLNDKPNLLA
                     APMNGSDLKYSTNTIASVYALIDVTPELSFKLTPAISITNFRSKAWLKKFEGNRFTGG
                     ADLKETYSESVDVNMESQVTFKKLFNNLHNVQATAVYTVRKQEVNIINGSANGFDFET
                     LNTLTNGGTPTSPRIVTGSVEELRMISYLARVMYDYKSKYYLTGSWRSDGISLFGPGH
                     RRGNFYSGSIAWKINEDFFKSVKQLDVLKLRLGWGQTGNSNIGSGFQYVNQISSPNSF
                     YAVFGDDQHIAKAMYTFEQMGNPSIEWEKAEMINSGFDFTLFKYRLTGSVEYYLKNNK
                     DLLVQVPPPLALGILPSSPYKPWFNAADIQNKGVELSLQWRDKVREFNYNIIGSLTTI
                     YNTVKYMPMPNITGEFNRTIVGQPIGSLYGYVSEGIIQQTEAYYDKNEDGTINLGAYK
                     FPKQEGVMPKPGDLKYSDLNGDGQVTALDKTIIGKTIPGFTYTLGFDCSYKNFDFSIF
                     LFGIGDFDIYNFQRATLSSMNTQDQMHNKLNDFAKNHWTIENASTKYVRVDPSNTNKN
                     DQISSFWVEDGSFLRIKDLQIGYNLPTKQTKTLGIGSVRIYANASNLYNFTNYKGRDP
                     EGFVSDNPLNSGVDRGAYPMPRSFTFGLQVGF"
     gene            complement(8989..11637)
                     /locus_tag="PLD44_03135"
     CDS             complement(8989..11637)
                     /locus_tag="PLD44_03135"
                     /inference="COORDINATES: protein motif:HMM:NF023636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HOF98365.1"
                     /translation="MKSYKIRTLQIILLISLYINASSKPILNETYEANSRVRSGIAIG
                     GIGTGSIELRKDGNFYNWTIFNNYPLSTGRFFSIAELPGKNSDKSLLFFLVRYQVEGQ
                     APKLKLLQLNNSLQEGGLEGIAYYYPWMSGIEKIQYSARFPFSNLVFTDSEMPFDIEM
                     EAFSPFIPHDVKNSSLPGAYFNFKITSRTTKKVDVMIVGSLRNLVGYDLIRKAFSSEL
                     VNEPDYKFFIHSASGMDETHDTFGQMGLGALGGNEVSYYLGWEHKHPYYEKLLIENRL
                     ANIDDTENRNYQLENITLGRMGSTDNDQRCFSSIAVSKILDAYNSVNCTFFMNWHFPN
                     QYGAIQGKMNGTPSLSEREKQNFTVNLERTKFIGKYYSNYFSDIKSLADYFVANETDL
                     KNRSKSFIEDMYTSDIELFVLDQINSHMNTFITSSTLTKNGQYAIREGLTTAQAWGPN
                     STSDVLLYASPMIIALFPDLQKSMMRTHAKLQTPDGEINHGLGFDPDYNQNGTWGVYE
                     RVDLVPNYIQMVLRDYLFTNDINYMKEMWPTIVKGINYILTKRDTNGDMMPDMHGIMC
                     SYDNFPMYGLASYIQSQWIAALRMASIVAVDMGDKTLAKKYANIAKKGSELMDQKLWN
                     GSYYNLSNDYTGNKGIDNGCLTDQLIGQWVAHNAGIGYLFDRQKVQKTLQSIMQKSFM
                     HNSFLRNCSWPATPELFPIHTSDLWVDQANTPWTGVELAFASFLIYENRFCEALKVIK
                     AVDQRYRKAGLYFDHQEFGGHYLRPMSAWSILNAYLGFSTNRGNLTFRPGIESDTFRL
                     FFVTADATAIYSNHPDKISIEIRTGELKFRSIKVEKYTGNNRSPMLYINNNKIKNQVV
                     EKTGDTIQISFNQIITVEKGSLIELK"
     gene            complement(11663..13627)
                     /locus_tag="PLD44_03140"
     CDS             complement(11663..13627)
                     /locus_tag="PLD44_03140"
                     /inference="COORDINATES: protein motif:HMM:NF025861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HOF98366.1"
                     /translation="MKGTSLKYFLPAIIVVFCFQQVLSSNSILSLYSPDKNIKAEVRF
                     KSADNNRQVLTYEIVRLENKNSIQLLESSPLGISRNDQSFTDNLKLTSCSKVRRINES
                     YSMTIGRHLKLKNCANEQKLTFKNDSGSTLEIIFRVYNNGVAFKYVFPDNSDKKVKVI
                     SESTSFNVPDNGVCWIQPYDKPGMWTPGHELYYKNEIPIGTKSPNSEGWAFPALFKVN
                     NNWMLISEANLTKNYCGTRLAQDATNGLYQIRFPEPADGEGVGEVYPSSTLPWEMPWR
                     FIAIGESLDDIYNNNLVSDLSNKAINKDFSWVKPGRASWSWISAPDSPCDYNALKKFV
                     DLSAEMGWEYSLVDANWDTMTGGSLQQLVKYAAQKDVGILVWYNSGGPNNKVPERPRD
                     LMTDRNIRREEFQKLQQWGVKGIKVDFWHSDKQLIIQQYIDLLSDAADYHIMVNFHGC
                     TIPRGWSRTYPNLISMESVKGAECYLFDSTYTDNAPVQNTILPFTRNVIGPMDYTPLV
                     LKGFKIPHKTTFAHELALTLVFQSGILHFAEDAGFLNQQPDFVKTFLKTIPVTFDESH
                     LLSGYPGKDIIIASRKGKTWYLAGINGENKLKNFDIKLPFIRGNCKINIIKDGDNAHS
                     FENIVMDYKSGQNLQLSTIPFGGFVIKITK"
     gene            13847..15109
                     /locus_tag="PLD44_03145"
     CDS             13847..15109
                     /locus_tag="PLD44_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOF98367.1"
                     /translation="MFKFLKQIDNKSYRPVFGALDIFKFIGPGLLVTVGFIDPGNWAS
                     NLAAGSNYGYSLLWMVTLSTIMLIMLQHNVAHLGIASGLCLSEAASIYLKPAYSKFVL
                     LSAMMASVSTSLAEILGGSIALNMLFDIPVRAGALLVSAFVLIMLFTNSYKVIEKWII
                     AFVSIIGLSFLYELSLVNIDWNHAVVGWVTPSFPNGSMLIIMSVLGAVVMPHNLFLHS
                     EVIQSRQWNLTDDKTIKRQLKYEFLDTIISMLIGWAINSAMILLAAATFFETKTPVTE
                     LEQANHLLAPLLGKNASVVFAVALLFSGISSSITSGMAGGTIFAGMYKEPYDIKDNHS
                     RLGVLLSLLLAAGIIFLISNPFQGLIVSQMLLSIQLPFTIFTQVYLTSSEKVMGKYKN
                     TTFSKILLYLLGVIVSVLNIFLFISFFK"
     gene            complement(15122..15706)
                     /locus_tag="PLD44_03150"
     CDS             complement(15122..15706)
                     /locus_tag="PLD44_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOF98368.1"
                     /translation="MLSHLEKYQIILASKSSRRQDLFKGLNIPFEIYPIEVDESFPPE
                     LIGMDIPMYLSKKKAEACTDIMKENTMLLTADTIVWYQGQVFGKPADRNAAKFMLTTL
                     SGKTHDVITSVSITTMKKQRTFGVASEVRFARLAPEEIDFYLDNFQPYDKAGAYGVQE
                     WIGFVGVEYINGSYYNVMGLPMQRLYNELKNWKE"
     gene            15840..17246
                     /locus_tag="PLD44_03155"
     CDS             15840..17246
                     /locus_tag="PLD44_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOF98369.1"
                     /translation="MLSNYFVSLIKAQLPFEPNEQQNDLLEALGRFLLSSEKRKIFLL
                     KGYAGTGKTSVVGALVRALNKIGHKTVLLAPTGRAAKVFANYSGTPAFTIHKKIYRQK
                     SLTDFHFQLAENLFSDVLFIVDEASMISNTDFDSVFGSGNLLDDLMEYVFSGRNCSLM
                     LLGDSAQLPPVGQAESPALDVGILKQYRLNVFEFTLTHVVRQALKSGILHNASLIREK
                     LTNKNLVRFPPFQLEKFADISRLSPAELTDEIQKEYAGAGVEDTMVITRSNYRANYYN
                     NGIRSRVMMKEEELSNGDLLMVTRNNYFWPKYYPEIDFIANGDILEVLRIRKFTEMYG
                     FRFVDATVRSLDYDWEIDTRIWLDTLNAETPAKAQQMNKILFEKVAEDYSEIKQKRQK
                     VKKIYENEFFNALQVKFAYAITCHKAQGGQWKKVFVDPGRISDEQINDDFYRWLYTAL
                     TRATENVFLVNFPMKFFS"
     gene            17481..19157
                     /locus_tag="PLD44_03160"
     CDS             17481..19157
                     /locus_tag="PLD44_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98370.1"
                     /translation="MKSSASHFLVAIFLSVLILPSCMNDVDLTNISDKVQWKGSLAVP
                     IGEAALTLEDLLAQIDSQNILVTESQEILYQDSDSIVSRFRDVDLAQYSQSLVKSYYV
                     SPIVRTIPANTIFPTLNDNVIIDLGINNSPSAERVDSAVIYSANLSVKVNVENININP
                     DNLKLTLEFPTDKVRTKNGSPIKIEFTPGAFNHPENVTIRDFLINTSGNASGIPVTLK
                     LDITTGSSPVTVGPSSVVGVELKFTGFNYKVAYGYFTPAVIATKILTQDLDLPSFTDD
                     GSFLFANPKVFLTVKSNIGAYLKFNVDYVKAYPKSDSSQVVYASFNGSRSTAESLDNK
                     PAVPGLWIAKKLRTLDKDFGATDLLFSNENKPDVLEYKYSVDIDGSKNDGKPDFITPD
                     GNIKVLYKIQIPLYLKPGSYYEFKDTIPDAGEDINSALEDVDVNSGSIFLTVTNGLPV
                     KGKLTLVLKDSLGRDISTTFKKEYDIDAPQVDAEGFVVESSITPQKIEIALNNDQLND
                     FKRSKDIVYKFRIDSKDANSFIHFRKSDTFKVKLGLFVNGKVSYDSNNND"
     gene            19173..21632
                     /locus_tag="PLD44_03165"
     CDS             19173..21632
                     /locus_tag="PLD44_03165"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOF98371.1"
                     /translation="MKKIIISILTGICCLTCIFSTSAQQVNSLYFMENSPIRSYLNPA
                     FQPTGSFYLGLPVLGYTYLSLGNNSLTLKDLVYNKNGETIWFLNPEGDVDKFYKSLRS
                     STLLNTNEQLNLLNFGFRAGSAYWTFGITEKVEGNLALPKDMFKLLLYGTPEQENNYF
                     NFKDLNANVMAYTEFALGYSKIINDQWTVGGKLKFLAGNAIVTSSNDYFNLNAGIEQW
                     NMKGTGSLNIAVPGEVTIGDKFESIDYTEPEDMQSWLKPSGMGLGFDLGFTYKPLENV
                     TLSASLIDIGTIKWNKNMKKVAYNMDYTFDGLAKIDNLDSVDMDAFLDTLSNAFKNSF
                     TTEEKATAFSTATSPKFNLGAEYAFANNKLSLGLLSTTTKVKSNMYQELTAAFNMRPS
                     NWFNLSLAYSLLNGRMSNIGAALGIRTGFFYWNLSSDYVSLDNAPLLLSDIDSSLPST
                     KIPIPYNSKGLNLALTVNFVFGNKVDKDHDGVVDKKDKCPDTPKAARKKVDANGCPLD
                     TDQDSIPDYLDQCPDTPLAARGFVDSKGCLLDTDNDSIPDYLDKCPDTPLGVAVDSLG
                     CPLDTDKDSVPDYLDRCPDTPLEAHGFIDENGCLLDTDQDGVPDYLDLCPNTPLEAIN
                     FIDKNGCPMDSDSDGVPDYLDQSPNTPLEARGLVDEKGAPRDTDGDGVYDFKDDCPKI
                     PGDSTNHGCPEVKKEVRTLFQRALKGIQFDTGKDIIKPSSYPILDQIAQVLKENPTYL
                     VEIQGHTDNVGNPQKNMELSEKRAIAVKKYLVSKGVEENRITAKGYGDTMPVASNKTA
                     AGRALNRRVEFIVTFEEVKFE"
     gene            complement(21725..22447)
                     /locus_tag="PLD44_03170"
     CDS             complement(21725..22447)
                     /locus_tag="PLD44_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOF98372.1"
                     /translation="MDVQLFIPCYIDQLYPQTGFNVIKILEKVGCRVHYNSNQTCCGQ
                     PAFNSGYWKETAYLAEKFLCDFNSDMPIVSPSGSCTGFILHHYNKLLANNPELLQKFK
                     EIKSQLYELTDFLVNILKTEDLGAVFPHKITFHDACSALREYGLKDEPRKLLKQVEGL
                     QLVEMDENDTCCGFGGTFAVKNHAISTSMTARKVANSLRTEAEFIVSTEASCLMNINT
                     YCKKHNLSICGIHLADVLASGY"
     gene            complement(22479..23525)
                     /locus_tag="PLD44_03175"
     CDS             complement(22479..23525)
                     /locus_tag="PLD44_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOF98373.1"
                     /translation="MKSSITSGKKRVFLATTICFLFAVGLYCQEKRNFKIVSYNVENY
                     FDCVDDSLTDDQEYLPNGIRAWNTTKYKKKQANIGKVICAIGGWESPALVGLCEVESE
                     KCLVDLTRYAGLKNLKYKFLHHESPDARGVDVALLYQPYVFKPFHDEAIRINFPQFPE
                     IKTRDILFASGIIPSGDTLHVFVCHFPSRLGGELESEERRIFVASVLRSKVDSLFSSN
                     NKANIVIMGDFNDYPPNRSLSETLKALPPTEPFLQKNLYNLMFPMHLKGKGSHKHNGE
                     WGTLDQIIVSGNLLTPTNSVYTLPSDAHIFEADFLLEDDRTFLGKQPFRTYVGMKYQG
                     GFSDHLPVYVDLWY"
     gene            complement(23567..25045)
                     /locus_tag="PLD44_03180"
     CDS             complement(23567..25045)
                     /locus_tag="PLD44_03180"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOF98374.1"
                     /translation="MFKFKKTVFFFTKLIILWAIINFFSSCQEDFTTNPADTVLFSTD
                     TLTFDTTFTSIGSATSRIMVYNPNNKAIRISSIFLASGKSSAFKINVDGKNNENNQFA
                     DIEIGAKDSLYIFVQVNVDPLDNNLPLLIEDALIFLVNGNIKTITLQAIGQDAIIFRN
                     KLIANDTTLTAEKPYLVFGYLAVDSAKTLRLDPGVKIYFHNNANLIVYGNLMADGTFE
                     KPITMRGDRFDKIKFETPVLYNVVAGQWGGIYLLWNKGKHVLHNVNLNSGYVGIYFSN
                     QDKNQLPELEISNCRIHNFLLYGLVVQNGNVKVLNSEISNSSSFCVYLNGGQHTFIHC
                     TIANYFNNSSVQPILRDKEPAVMIMDLNRIAPMRTTFLNCAIAGSMENEFTLATRFPK
                     LYKGTFSHCYIRRPNALDLPQFSNIRWSEKNDTVFKNIYFDYKKGTYFNFEPDSVSPL
                     RGLADYNIAAENCPVDLNGINRLQDGAPDAGAYEWMPAKKDE"
     gene            25175..25444
                     /locus_tag="PLD44_03185"
     CDS             25175..25444
                     /locus_tag="PLD44_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98375.1"
                     /translation="MASRKELKKQINDLTGQLALEILFRGTVMQVLEPEKMYALLSKV
                     MIMNRQFISRVNAAKRIKSSKEVKDFYQQFFTDWEKNVGEIIQEF"
     gene            complement(25484..26197)
                     /locus_tag="PLD44_03190"
     CDS             complement(25484..26197)
                     /locus_tag="PLD44_03190"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOF98376.1"
                     /translation="MLKNNIFAFIFILTFCSVFFPKVKAQGNLPYVDDKLIHFGFSLG
                     LNTADFGITPRDTLINGSRYYTDVSALQPGFSVGVITNLRLARYWNLRFNPTLHFSER
                     DITYRKDTTSTTGSTTVNSIPLSFPVYLKYSAERKDNYRPYLLGGGGVYFDFGRNKEK
                     PILLKPMDYFVEFGVGCDLYFSFFKLAPELKFAIGFNNMLTPLEQRNAGGLTEEDKIY
                     STAISKLTNKMITLSFNFE"
     gene            complement(26320..26712)
                     /locus_tag="PLD44_03195"
     CDS             complement(26320..26712)
                     /locus_tag="PLD44_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98377.1"
                     /translation="MKKILFLLFLFSNSALIGQQIYNAAGKPIGKIIGQNYYDNNSKL
                     KGKVESVFVYDAAENLIGKIDGSYLYDGNGKQIGKVENNDFYDALGTKLATVSGNALY
                     DSYGRRIRKFDGLTLSQIIVYYYYFSNL"
     gene            26958..27479
                     /locus_tag="PLD44_03200"
     CDS             26958..27479
                     /locus_tag="PLD44_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOF98378.1"
                     /translation="MNRLDAVFFASDAITVARQLLGKTLVRKWEDGTISRYKITETEA
                     YFGEEDKACHACKGKTQRTQIMYAEGGYIYVYLIYGIYWLLNVVTGNKEHPEAVLIRG
                     INNISGSGRVGKELKLDKSFYGENLISSDRIWIEDAPEALYFFTATRIGIDYADEEWK
                     SKPWRFVMTSSEK"
     gene            complement(27616..28095)
                     /locus_tag="PLD44_03205"
     CDS             complement(27616..28095)
                     /locus_tag="PLD44_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOF98379.1"
                     /translation="MKKTYTTDELFLKAASYCSISEHCVSEVEEKLSHWNVSENEKRK
                     IIDRLIKENFINEKRYAQSFVRDKFRLNKWGKIKIELALKSKGISNDEIKSAISTIDD
                     GEYQEMLASALKSKLSMLKYDYEYEKQGKLFRFAQSRGFETSIIDRVLRSLNQSAKE"
     gene            28264..29628
                     /locus_tag="PLD44_03210"
     CDS             28264..29628
                     /locus_tag="PLD44_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOF98380.1"
                     /translation="MDTKGAPITLGTEKIGKLLIQYSLPAIIAMTASSLYNITDSIFI
                     GHGVGAMAISGLAVTFPFMNLSAAFGSLVGVGAATLMSVRLGQKEYGTANRILGNVFV
                     LNVIFGVAFMTVSLIFLDPILKFFGASDTTLPYAHDFMVVILIGNVFTHIYLGLNGIL
                     RSIGKPGKAMYATLLTVLINLILNPLFIFVLKWGIKGSALATVISQIVMLAWQIRHFS
                     NKNNFVHLQKGIFRLDKKIVLDSLTIGLSPFIMNAVASVIVIVINQTLIRYGGDLAIG
                     AYGIINRVSFLFVMIVMGLNQGMQPIAGYNFGAKNFNRVNEVLKLSIFIAMGMMTLGF
                     IIVEFFPHAVASLFTSDKTLIQLSIEGLRIVFVLYPMVGFQMVITNFFQSIGMSGKAI
                     FLSLTRQLLFLLPFLLVLPTFYGLKGVWYSMPAADFLATIVAAYMLFVNFGSFIKNQN
                     RDHK"
BASE COUNT         8874 a         5498 c         5542 g         9736 t
ORIGIN      
        1 tgaaaataat aagggaaata atttacaaaa aagaatctat attgttttgt gaaattcgaa
       61 agacttgttt gtaaacaatg ttttattttt atcaattaat ttacaaattg tcaaaacaga
      121 ctgtttactt gtatatccat cagagggagt atttaaacaa taatcaagta atttatcaat
      181 agtagatgtt gctacatgca tgcgagtatc agaagatgca tagtaaatgt acaccgtacc
      241 atctgcgtcg gctatccatc cattactaaa aagtacattt gacacatcgc ccacccgctc
      301 atcatcttcg ggtgccataa aatatccggc gggttctgca atcaatttag acggatcctg
      361 taagtcggta agatataaat aaagcacata ccgaagtccg gaagcacaca tacgtacccc
      421 gtgagccaaa tgaagccagc cctttccggt tttcagtgga tgcggtccct cgccgttttt
      481 aagttctttt atcgtgtgat aataacggtg attaataatt ttttcgttcg taataactgc
      541 attctccatg ctgtccacca aagcccaacc aattcctcct ccacttccgg gatctataaa
      601 actgtcctga ggacgtgtat aaagggcata tttaccattt acaaattcgg gatgcaatac
      661 tacattgcgt tgctggctct tcgaaactaa attcgggaga cgttcccatg tattcatatc
      721 tttggttcga acaattccgg cttttgcaat agcagatgtt aaatcacccg gagctgcatt
      781 cggatcttta ctttcgctac aaaaaatacc ataaatccaa ccatcttcat gagctgtaag
      841 cctcatgtcg tacacattgg tttcagaact tccttcgggt acttctaaag aaagaggttt
      901 ttctctgaat cggaaattat caacaccatt attgctttcg gcaatagcaa aatacgattt
      961 gcggtcaaca ccttccaccc gaacaaccaa gacgtatttg ccattccatt taattgcacc
     1021 ggaattcaaa gttgcattta ctccgattcg ctcaagcaaa tatggattcg tttgtttatc
     1081 aaaatcatat cgccagcaaa gaggaatatg ctctggtgta atgataggat aagcataacg
     1141 attataaata ttattttttg acggtaatat ctcgtttttc tgtgaaagca gaatttcctg
     1201 ctgttttatt tttctttgca aactattgtt gaattccata tttttaaata tatctgatac
     1261 acgcaatctg tcctaacctt tttataaata attcagataa aaatcaatat aataaaagct
     1321 ttgtatgcgt ttaaaaagct aattttatta taaagcgggg ctgaaaacgc atatcaaccc
     1381 cgcttcaatt ctatccaatt taaaaaagat tgactatttt acaattttca ttgtttcacc
     1441 attggcttta actatataaa cgcctttagc caaatcgcta atattaactt cagttacatt
     1501 tctgaattct tttatctgtt ttccggctaa gttgtaaatt gtaccatttg ccaattcgga
     1561 caattcaatt tttgtggctg ttttgtttac gaatatactt ttggtagcat caggattttt
     1621 aacggcagaa ggaacatctg gagctaagaa acctgcatac caattaagat accaagcgtc
     1681 atcagtgcgt gcattccacc agtaacttcc tgtttgatct ttattattag catccttagg
     1741 atcagcagca tcatagtcgt tatacttcat gtcgaaggaa atacccttac ctgcattcag
     1801 atttttccag atattaacat caaaatcagg tctaaaaaca cctgtcagtg cggggaaccc
     1861 aatggtgata atccgtttaa ctaccccact gccggttgta gctcctttat catcaaccaa
     1921 taagttggca gcaagtagtg catttgttcc tgcccagtca acagatccgg tccctgctgc
     1981 atcaaaatta atcatcattg catccctaaa agcactggca gtaaatgtag ccttgagtcc
     2041 accaaaagcg gcaaaacgca tgtaagcgtt actctttttt gctccttgag ctaatgctct
     2101 atcggcaatg gcttgtattc ttaagtcctg cgcccaggct atttctacaa catctttatt
     2161 gttaatgtcc tcatcggtgc attttatcaa aacataaata ctgtaatcat cgtaaataac
     2221 tttaaaagca ccagtaaagt cagcaggtcc attatcaaca ccagctttag caatacgttt
     2281 ttcaggtata ccccccagtg caaactcagt gcctgcaaca tcccatacag catcaaaatc
     2341 agccgatgca gcatcgaaat tagcaggagc ttgagttgct tttattgctg cacggatata
     2401 ctttgcctga tcggcttcag cttcgagaga atcagtttgc aaccaattat aaatagggaa
     2461 tttccagtta gcaactgctg ttacatcatc agccattgaa gaaacggcaa ataccatcac
     2521 aagaaaaaat aaagttaact ttttcataat acttaaattt taaaaattaa taattaagaa
     2581 atctgttttt aaattattgt ttataaataa cttgtttttg aatagaattc actttttaat
     2641 tactattaca gtatagctgt ctccaaccga agtgcctttg taaaaagtgt acactaaagg
     2701 tgttgaaaaa tcattttcgg ttacgccgtt aatctgcgtt acatttccga tttttacagt
     2761 tcctactgaa ccagtccatt tagctttcag ctttgtaata tcagtaactt taagcacatc
     2821 aatagttatg gttttattgt ttttatcaat aactccgacc ggagcaggaa caagtccttc
     2881 gaacgtaaac tcgataacag ggtcttgtgt cgtcaaaatc agtttataaa attgtttggt
     2941 agagttctct gccacgactt caaccccagt taaggttgtc aaattatacc tgcgtgtaga
     3001 actgtatggt atgccattta acaacactgt actttttggt gatgatgaaa cggttaacct
     3061 taccgacgtt gcattggttc cataattcaa cgccaaattt atctctttat tagtttcgtc
     3121 aatcgtagct atttcaccat ttgagttcgt ataacctaaa ttgggtgagt ttgggaacaa
     3181 ttgcagcgat agtaactgct tttcgtttga tgccgggaaa gtagaaacat aaactgtgta
     3241 aatattttcc tctccgtctc tcgatttaac ggtatagact accggatttc ccggcgaaaa
     3301 attttgcaca gaaacaccag atgtttgttc ctctcctgag acataaacct ttgcatcagg
     3361 tgaattgaca gtaaatacag gtttgaagtt aaaatcggta atgttgtcgg ggaatgaaaa
     3421 cagtatttgc ttatcagctg taattgtacc ggttacaccg ttcattacaa atgagttaaa
     3481 ttcgttacga atatcgataa ctgttattgg aactgttttg gtcctacgct gaattgattt
     3541 cccaaaatcc gaagttgtag tagcaacaac agtcaggtta taagtacctg cagttggata
     3601 ggttacaggt aacgaatcgg caagattaaa atctacaccc tttgcaccta ttgaatccca
     3661 ttctgctcca ggttttccat cgtataaagt gagaaattca gcacttcctt taactttcac
     3721 ataaaaggtt gtgcctgcaa atgcattatc aaatccctgt tcctcgaaag aaagactaaa
     3781 ctgggggtcg actattactt catcttcaca tgctgtaaaa aacaccaaca ataaaatagt
     3841 aaaaaaaata tgtcttgttt tcatttgatt tatcagaatt ataaagttaa ttaccaacct
     3901 gcattttgtt tgagagcacc tttgtttgca tctatttcgt attgaggtat tggaaaatac
     3961 aaatgtcgtt ttatattaaa cttgtcactt accatcgtgg cgaagtttat attaaacctg
     4021 ctatcgtaac tccatcgaat aatgtcgttg taccgatgtc cctccgttgc caattccaca
     4081 tctcgttcgt gcataatagc tgagggcgtg agttcagaaa ccggcggcat atctactctg
     4141 gctcttacct cattcagagc ttttaaacct tcatcgaggt cagagcctga ctgaaagcat
     4201 gcttcggcat acatcaacaa aacgtcggaa taacgtatta ccctgaaatt aacaggtgat
     4261 gctgctggag cactcccatg attataggta ggatagaaat atttttttat acctatggcc
     4321 ttactcaatg taaatcctga gtaaacagtc gggaatatac ctccttttgc atttacagtt
     4381 ccatcgaagg gtttagcata gcccgattcc ggtcgccaat ccatcatatc gccatccaaa
     4441 tagcaggttg cataagcacg agggtctttg gcatatccgg ccggatgtga gcttactgat
     4501 tcaaatgtgt tccataacga agggtcaata accatattac gatagccgtt ttggtgagtc
     4561 gaaaaataga gcgaaagcat attacccccc ataagatatg caggaaggta cgtattggtt
     4621 cccggattat cgttgtactg tatttcgtaa acagattctt gattgttttc agctttgtat
     4681 atcgttgaac ccacaggtat gtcgacaaag gaaaaattgc ataaaagcgc tgcctggtaa
     4741 tgttctttgg tcttaattgc aggtctgcta agactgtagt ttccgctttt gattatatta
     4801 tttaaaacac tttttgcttt ttcaatattt gcctttgctt catccgtgtt tgctttatca
     4861 aaacgtaaat aataatgata gtgtttatac aataaagctt tacctaaaat cgcacttgcc
     4921 cctccactgg ttattcgtcc aagatcttga ttgggccatg aagcgggtaa attggctatc
     4981 gcaaaatcta aatctgcttc taattttgac caaaattgtt cgcgggtacc atttctgtac
     5041 gatttcagag catcggtagg tacatttgtt tcatcataat aaactggagt atcgaaaagc
     5101 gttaccaaat aaaaataact taatccacgt aaagctttca actgagcctg gtactgtttg
     5161 tatttgtttt cttctatttt atctttcaac ggagtcattt tgctcagtat atctgaagta
     5221 cggaataaaa cgtaataaaa atcgaggtac atctcctgca aatctgcatt tgtgggactg
     5281 aaattcataa tatcaaatcc actctgacct tcgaaccaga tatatgaatc aagagaatta
     5341 aatatcttgt aataattcaa tccgaaaacc ccataatatg caaatggagt atatgcggca
     5401 ttaattgcat aaataagctc attttcattc tttaaaaaag aagtattgtc aagattattt
     5461 ttgttcacgg tgtccagaaa atcgtctgaa caagcggtaa ctatcaaaaa tgacagtaat
     5521 agtattacta attttttcat atgatttttt ctgattagtc tgtttttctt gtgttattta
     5581 aaagccaacc tgtaatccga aggtaaacga tcttggcatt ggataagccc ccctatcaac
     5641 gcctgagttt aacggattat ccgaaacaaa tccttcaggg tctcttcctt tataatttgt
     5701 gaagttataa agatttgatg cattagcata tattcttaca gagccaattc ccaaggtttt
     5761 tgtctgtttt gttggcaaat tatagccaat ttgtaaatct tttatgcgca gaaaacttcc
     5821 atcttctacc cagaaacttg aaatctggtc atttttatta gtgttactag ggtctacccg
     5881 cacatatttt gttgatgcat tctcaattgt ccagtgattt ttagcaaaat cgtttaactt
     5941 attgtgcatt tgatcctgcg tattcatgct cgataatgtt gcacgttgga aattgtaaat
     6001 atcaaaatct cctattccga aaaggaaaat actaaagtca aaatttttat aagaacaatc
     6061 aaatcccaat gtataggtga aacccggaat tgttttacca ataatagttt tatcgagtgc
     6121 ggtaacttgt ccatctccat tcagatcact gtactttaaa tcaccgggtt taggcattac
     6181 accttcttgt tttggaaatt tataagctcc gagattaata gtaccatctt cgttcttatc
     6241 ataataagct tcggtttgct gaataatacc ttcggaaaca tatccgtata gcgaaccaat
     6301 gggctgacct actatcgttc gattaaattc acctgtaata ttcggcatag gcatatattt
     6361 cactgtatta tagatagttg tcaaacttcc aataatatta tagttaaatt ctctaacttt
     6421 atctctccac tgaagcgaaa gctcaacacc tttgttttga atatcagcag cattaaacca
     6481 tggtttataa gggctgcttg gcaaaatgcc caatgccaaa gggggcggaa cctgtaccaa
     6541 aagatcttta ttgtttttta aataatactc taccgaaccc gttaatctgt atttaaataa
     6601 agtaaaatcg aaaccagagt taatcatttc agctttttcc cattcgatag atggattccc
     6661 catttgttcg aatgtataca tagcctttgc tatatgctga tcgtcgccaa aaacggcata
     6721 aaagctgttt ggactggaaa tctgatttac atactgaaac ccacttccga tattagagtt
     6781 tccagtttgt ccccaaccca agcggagttt caatacatcc aactgtttta cactcttaaa
     6841 aaaatcttcg ttaattttcc atgcaataga gcccgagtaa aaattacctc tgcgatgtcc
     6901 aggtccaaat aatgatattc catcggaacg ccatgaaccc gtaaggtaat atttcgattt
     6961 ataatcgtac ataacgcggg ctaaatatga aatcatccgt aattcttcga cactgcctgt
     7021 tacaatacgg ggcgatgtag gtgttcctcc gtttgtcaat gtatttagcg tttcaaaatc
     7081 aaatccattg gctgagccgt taataatgtt gacttcctgt ttgcggactg tataaaccgc
     7141 cgttgcctgt acgttatgta aattgttgaa caatttttta aacgtaacct gactctccat
     7201 attgacatct acgctctcgc tatatgtttc cttcaaatcc gctccacctg taaaacggtt
     7261 cccttcgaat ttttttagcc atgcttttga acgaaaattg gtaatggaga tagccggcgt
     7321 taatttgaat gacaattcgg gagttacatc aatcagcgca tatacactcg caatagtatt
     7381 ggttgagtat ttcagatccg aaccattcat tggggctgcg agtaagttgg gcttgtcatt
     7441 caagagtgta ttcagatagc cattcccggt ttgaggtgtt gaagttagat tgccgttaat
     7501 atcgtaccag tattgcatct gataactttc gtaaccaccc agcaactcag gattgtaaat
     7561 acgcataaga ggcgatgtat taagatcgta aatagatgat tgcactgtca tgggtgtttc
     7621 gcgtgtactg taactcgcac tcaggttttc cccaatttta atgtgttggc ttaatttaaa
     7681 atcagaattt gcacgtatct gatagcgctc gtaattattc tttataactg ttccatcttc
     7741 gtttaaatag ccaaacgaaa tgttgaaatt gctgttttcc ccaccacctg caacagataa
     7801 attgtagttt tgattaaatc cgtttttaaa gatttcactc tgccagtttg ttccttgttc
     7861 ccaacctgct ccataatact tttgtcggaa ttcgggcgat aaaacatgtg caggattaac
     7921 agcattgtta taggttttat ccaaaccata ttcggggtac aaaacctgcg aatagaaatc
     7981 tgaatactgc tgagcattca ttacatcgta ttcaattggt ttcatgttca aactcaaata
     8041 cgatgaaaaa tttacctggg gtttccctga tttaccacgc tttgttgtaa taataataac
     8101 tccattcgaa ccatttgctc catatatggc cgatgcggat gcatctttca gtacctgaaa
     8161 agtttctata tcctgtggtt gaatactatt aatattgggc acctgaatac catcgaggat
     8221 aaaaatcgga tcagcgcttt tgtttataga accgacacca cgtacacgaa tagtggcacc
     8281 acctccagga gctccgtttt gcgaaacaag tacaccagga gcttttccct gcaaagcctg
     8341 agctgcactt gaagccgggt ttttggtaag ttctttggac gtaactacgg caacagatcc
     8401 tgttaaatct gattttttta ctgtagcata accgatagat acaacctcag caagttgagt
     8461 agctgattcc tttaatgtta catttataac ttttttatcg ccaacaggga tctcctgttt
     8521 atcatatccg gtaaaagaaa aaatcaaaat atctgaaggc gatgcctgaa tactgtaatt
     8581 tccttccaga tcgctcacag ttccaaccat tttacctttt aattggacgg ttgcaccaat
     8641 catcggctcg ttcgttgttc catcgataat tttgcctgta atattttgtg caaatatttg
     8701 tgagtttata attaagctaa ttataaataa acttattctc gaaaagtata atacttgttt
     8761 tttcatggtg gttttacaat aattattcag ctatttatta cttattctat aaatttagtt
     8821 ggcaataaaa gatttattta gggataacac gaaaaattta caatacaaaa ttaaattatt
     8881 gtgagtccaa aaacatcata cttttttaac caaagatgat acattttatg aaactttaga
     8941 aaaaatagat tcatgatttt ttagataaag tgtctgatga aaatagcact attttaattc
     9001 tataagactt cctttttcga ctgtaataat ctgattaaat gatatttgga tagtatcccc
     9061 tgttttctct acaacttgat tttttatctt gttattgttg atataaagca tcggacttct
     9121 attatttcca gtgtattttt cgactttaat actccgaaat ttcaattctc cagttcgtat
     9181 ttcaatactt attttatcag gatgattact gtaaatggca gttgcatcag cggtaacaaa
     9241 aaataaccgg aaagtatcgc tttctattcc tggtcgaaac gtcagatttc ctctgttggt
     9301 tgaaaaacca agataggcat ttagaatact ccatgccgac attgggcgaa gataatgacc
     9361 accaaactct tggtgatcga aatataatcc cgctttccgg tatctttggt caactgcctt
     9421 tatcacttta agtgcctcgc agaatcggtt ttcgtaaatt aaaaaagaag cgaaagcaag
     9481 ttccacacct gtccatggcg tgttagcctg atctacccat aaatcagaag tatgtattgg
     9541 aaacaattca ggggtagcag gccaactaca atttcgcaga aatgaattgt gcataaaaga
     9601 cttttgcatg atcgattgca gcgtcttttg caccttttgc cggtcgaaaa gataacctat
     9661 tccggcatta tgagctaccc attgcccaat taactgatcg gtaagacatc cattatcaat
     9721 tcctttattt cctgtgtaat cgtttgacag gttataatat gaaccattcc aaagtttttg
     9781 atccattaat tcagagcctt ttttggctat gttagcgtat ttttttgcca gtgtcttatc
     9841 gcccatatca acagcaacta tcgatgccat acgtaacgca gctatccatt gcgactgtat
     9901 gtatgaagcc aaaccgtaca tcggaaaatt atcgtaacta cacatgatac cgtgcatatc
     9961 aggcatcata tctccattgg tatcacgctt ggttagtata tagtttatac cttttacaat
    10021 ggttggccac atttctttca tgtagtttat atcatttgta aataaatagt cgcgtagcac
    10081 catttgaata taattaggca caagatcaac tctttcgtaa acaccccaag ttccattctg
    10141 attataatcg gggtcgaatc ctagtccgtg atttatttca ccatcaggag tttgcagttt
    10201 ggcatgtgta cgcatcatcg atttttgtaa gtcgggaaac aatgcaataa tcatggggga
    10261 agcatacaac aatacgtcgg aagtcgagtt tggaccccat gcctgagccg ttgttaaacc
    10321 ttcccttatt gcatattgtc cgtttttagt cagagtggac gaagttataa atgtattcat
    10381 gtgagaatta atctgatcca atacaaataa ttcgatatca gatgtataca tatcctcaat
    10441 aaaagatttc gaccgatttt tcaaatctgt ttcgttggcc acaaaatagt cggctagcga
    10501 ttttatatcg gaaaaataat tggaataata ctttccgata aacttcgttc gttccagatt
    10561 gactgtaaaa ttctgttttt ccctttcgga aagcgatggt gtcccgttca ttttaccctg
    10621 aatagctccg tattgattag ggaaatgcca gttcataaaa aaagtacagt tcaccgaatt
    10681 ataggcatcc agaattttag aaactgcaat agagctgaaa caacgttgat cattatcggt
    10741 gcttcccata cgccccaacg ttatattttc gagctgataa ttgcggtttt cggtatcatc
    10801 gatattagca agtctgtttt caatgagcaa tttttcgtaa tatggatgct tatgttccca
    10861 ccccaaataa tatgaaactt catttcctcc caatgctcct aaacccatct gaccgaatgt
    10921 atcatgtgtt tcatccatcc ctgaggcgct atgaataaaa aacttataat cgggttcgtt
    10981 gaccagttcc gaactgaatg ctttccgaat aagatcgtaa cctaccagat tgcgtaatga
    11041 accaacaatc attacatcta cttttttagt agttcttgat gttattttaa aattaaaata
    11101 cgcacccggt agagatgagt tttttacatc gtgcggtata aatggtgaaa atgcttccat
    11161 ctcaatatcg aaaggcattt cactgtctgt aaaaactaaa tttgaaaaag ggaaccgtgc
    11221 cgaatattgt attttttcaa taccactcat ccatggataa taatatgcaa taccctccaa
    11281 accaccttcc tggagggaat tatttagttg aagaagttta agtttgggcg cttgaccttc
    11341 tacctgatat cgaaccagaa aaaaaagtaa actcttgtcg gaatttttac ccggtagttc
    11401 cgctatgctg aaaaatctac cggtactcag aggataatta ttaaaaatag tccagttgta
    11461 aaaatttcca tcttttcgca attcaatact tcctgtacca ataccaccta tggcaatgcc
    11521 gcttcttaca cgcgaatttg cttcatatgt ctcattcaga atgggtttcg atgaggcgtt
    11581 gatatacaat gaaattaaaa gtattatttg aagtgttcgt attttatatg atttcatatt
    11641 tttgatcaga ttttgggttg tgttattttg tgattttaat gacaaaacct ccaaacggaa
    11701 ttgtgctgag ctgtaaattt tgtcctgatt tataatccat tactatgttt tcaaaactgt
    11761 gtgcgttatc gccatctttt attatattta ttttgcaatt tccacgtata aatggcagtt
    11821 taatgtcaaa atttttcagt ttgttttctc cgttgatccc ggccaaatac catgtttttc
    11881 cttttctaga agcaataata atatccttac ccggatatcc tgaaagcaga tgcgactcat
    11941 caaatgttac aggtattgtt tttaaaaaag ttttcacaaa atcgggttgc tgattcagaa
    12001 aaccggcatc ttctgcaaaa tgcaatatgc cactctgaaa caccagagtc aaagccagtt
    12061 cgtgagcaaa agttgttttg tgtggtattt taaacccttt cagaaccaat ggcgtgtagt
    12121 ccattggtcc gattacattg cgtgtaaatg gtaaaatggt attttgtaca ggcgcattat
    12181 cggtataggt tgaatcgaat aaatagcatt ctgctccttt tacggactcc atcgaaatca
    12241 aattaggata tgtgcgggac caaccccgcg ggatagtaca tccatgaaaa ttaaccataa
    12301 tgtgataatc agcagcatcg ctaagcaaat cgatatattg ctgaatgata agctgcttgt
    12361 cgctatgcca gaaatctact tttattcctt ttacacccca ctgctgtaat ttctgaaatt
    12421 cttccctacg tatattgcga tcagtcatca aatcgcgtgg cctttcgggc actttattat
    12481 ttggtccgcc cgaattatac caaaccagta tgccaacatc cttttgggca gcatatttca
    12541 ccaactgctg caaacttcca cctgtcatcg tatcccagtt tgcatcaacc agtgaatact
    12601 cccaacccat ttcggccgat aaatctacga attttttcaa agcattgtaa tcacacggac
    12661 tgtcgggtgc cgatatccaa ctccaggatg ctctacccgg tttaacccac gaaaaatctt
    12721 tgtttatagc tttattgctt aaatcgctaa ccagattatt attgtaaatg tcatcgagcg
    12781 attcaccaat agcaataaac ctccaaggca tttcccatgg caaagtagaa gaaggataaa
    12841 cctcgcctac cccttcgcca tctgccggtt cagggaagcg gatttggtac aaaccattgg
    12901 tggcatcttg cgccaatctg gtaccacaat aattttttgt caggtttgct tctgaaataa
    12961 gcatccagtt gttatttact ttaaatagtg cagggaaagc ccatccttca gaattgggag
    13021 attttgttcc aataggaatt tcatttttat aatacaattc gtgtccgggc gtccacatac
    13081 ctggcttatc gtaaggttgt atccaacaaa cgccattatc aggcacattg aaacttgttg
    13141 attcgctgat taccttaact tttttatccg aattatcagg aaaaacatat ttgaaggcaa
    13201 caccattgtt ataaactctg aaaattattt caagtgttga acctgagtcg tttttgaatg
    13261 tcagtttttg ctcattggca caatttttca attttaaatg acgccctatt gtcattgaat
    13321 aggattcatt tattcttctc accttgctac aagatgttaa tttgagattg tcagtaaatg
    13381 attgatcgtt acgactgata ccaagaggtg aagattcaag aagttgaatg ctgtttttat
    13441 tttccaatcg aactatttca taagttagca cctgccggtt gttgtcagct gacttaaaac
    13501 gaacttctgc ttttatattt ttatccggag aataaagaga aagaattgaa ttagaagaaa
    13561 gtacctgctg aaaacaaaaa actacaataa ttgccggtaa gaaatatttt aaggatgtgc
    13621 ctttcatttt ttggacatga atcgttttaa ttaaatcatg tataatcacg tattgcaaaa
    13681 ataactaaaa agccacttct atttgtgata tatttttatc aaactatgat attaaatcat
    13741 ttgtaaataa aattaaagcg taaaataaat aattcgcggt aaatggttgt ttaaacaaaa
    13801 tttgatattt ttgtccagtt tttcaaccaa taaaaagaat cgaaacgtgt tcaagttttt
    13861 gaagcagatc gacaataaat cttatcgtcc ggtatttggg gcattggata tttttaaatt
    13921 tatcggcccc ggtttactgg taacggttgg ttttatcgat ccgggcaatt gggcttctaa
    13981 tctggctgcc ggttcgaatt acggttattc gttgctgtgg atggtaactt tgtcaaccat
    14041 aatgcttatt atgttgcagc acaatgtagc gcatttgggc attgcatccg gtttgtgctt
    14101 gtccgaagcc gcttctattt acctgaaacc agcgtattcc aagtttgtcc tgctttcggc
    14161 catgatggct tctgtttcta cttctttggc cgaaattttg ggaggaagca ttgctttgaa
    14221 catgctgttc gatattcccg ttcgagccgg tgctttgctg gtttctgctt ttgtacttat
    14281 tatgctgttt accaattctt ataaggttat cgaaaaatgg atcattgctt ttgtctccat
    14341 tattggactt tcatttttgt atgaattgtc gttggtaaat atagattgga atcatgctgt
    14401 tgtgggctgg gtaactccaa gttttccaaa tggctccatg ttaattatta tgagcgtgtt
    14461 gggagcagtg gtcatgccac acaatttgtt tttgcattcg gaagtgattc agagccgcca
    14521 atggaatttg accgatgata aaaccatcaa acggcagttg aaatacgaat ttcttgatac
    14581 tataatttcc atgcttattg gttgggctat caacagtgcc atgattttgc tggctgccgc
    14641 cacttttttt gaaacgaaaa cgccggttac cgaattggaa caagccaatc atttgctggc
    14701 tccgttgctg ggtaaaaatg cttctgttgt ttttgccgtt gcattgttgt tttcaggtat
    14761 ttcctcttcc attacttccg gtatggccgg aggaacaatt tttgccggca tgtacaagga
    14821 accttacgat attaaagaca accattcgcg actgggtgtc cttttatcac ttttactggc
    14881 agcaggtatt atctttttga tttccaatcc ttttcaaggg cttattgtct ctcaaatgtt
    14941 attgagtatc caattgcctt tcaccatttt tacgcaagtg tacttgactt cgtcagaaaa
    15001 agtaatgggg aaatataaaa ataccacttt ttcaaaaata ctgctttact tgttgggcgt
    15061 tattgtttcc gtactgaata tattcctgtt tatcagcttt ttcaagtaag atttttggtt
    15121 tttattcttt ccagtttttc agctcattgt acaatcgttg catgggtaga cccattacgt
    15181 tgtagtagga gccgttaata tattcaactc ctacaaagcc aatccattcc tgtactccat
    15241 aagcgccggc tttgtcgtag ggttgaaaat tgtcgagata aaaatcaatc tcttcaggtg
    15301 ccaatcgtgc aaaacgaact tcagaagcca cgccaaaggt tcgttgtttt ttcatggtag
    15361 taatgctgac gctggtgatg acatcgtgcg ttttacccga cagcgtggtt aacataaatt
    15421 tagctgcgtt tcggtcggcg ggttttccga aaacttgtcc ctgataccaa acaatggtat
    15481 cggctgtaag cagcatggta ttttctttca taatgtcggt gcaagcttcc gcttttttct
    15541 tcgatagata catgggaatg tccatcccta taagttcggg aggaaaagac tcatctactt
    15601 caatcggata aatttcaaaa gggatattca atcctttaaa taaatcctgt cttcttgacg
    15661 attttgaggc aagaataatt tggtattttt ccaaatgtga aagcaatttg aacatttttt
    15721 tcggtttgaa ggattgtttt tgttctgcta ccgaattact ccggatatta ttgctacaaa
    15781 aatatgattt ttttttgttg tgtagcttat tttgcaaaaa tatcgtttaa atttgccgta
    15841 tgttaagtaa ctattttgtt tcgctgataa aagcgcagtt gccttttgaa ccgaatgaac
    15901 agcaaaatga tttgcttgaa gcattgggaa ggtttctgct atccagtgaa aaaagaaaaa
    15961 tttttctgtt gaaaggttat gccggaaccg gtaaaacttc ggttgtgggt gctttggtca
    16021 gggcgttaaa taaaattggc cacaagacag ttttactggc tccaacgggc agggcggcaa
    16081 aagtttttgc aaattattcg ggaacgccgg cttttacgat acacaagaaa atttatcgtc
    16141 aaaaatcctt gaccgatttc cattttcagt tggctgaaaa tcttttctcg gatgtgcttt
    16201 ttattgtgga tgaggcttcg atgatttcca ataccgattt cgattcggtt tttggttcgg
    16261 gtaacttgtt ggacgatttg atggaatatg tttttagcgg acgcaactgc agcttgatgc
    16321 tgttgggcga ttcggcgcag cttccaccgg taggacaagc cgaaagtccg gcattggatg
    16381 ttggtatatt gaaacaatac cgattgaatg tttttgaatt tacattgacc catgtcgttc
    16441 gtcaggcgct gaaaagtggc attttgcata atgcttcttt gataagggaa aagctgacaa
    16501 ataaaaattt agttcgattt cctccttttc agttggaaaa atttgccgat atttcccgct
    16561 taagtccggc cgaattgacg gatgaaatac agaaagaata tgccggcgca ggagtggagg
    16621 atacgatggt cattacgcgt tccaattaca gggctaatta ttacaacaat ggcattcgct
    16681 cgcgggtgat gatgaaagaa gaggagttgt cgaatggtga cttgctgatg gttacgcgta
    16741 acaattattt ctggccaaaa tattatcccg aaatagattt tatagcaaac ggcgatatac
    16801 tggaagtttt gcgtatcaga aaatttaccg aaatgtatgg ttttcgattt gtggatgcca
    16861 ctgtgcgctc tctcgattat gattgggaaa ttgatacccg catttggctg gataccctca
    16921 acgcagaaac accggcaaaa gcgcagcaaa tgaataagat attgtttgaa aaagtggcgg
    16981 aagattattc tgaaattaag caaaaacggc agaaagtgaa gaaaatttat gaaaatgaat
    17041 ttttcaatgc ccttcaggtg aaatttgctt atgccattac gtgtcataaa gcgcagggcg
    17101 gtcaatggaa aaaggttttt gtcgatcccg gaaggatttc cgacgaacaa atcaatgacg
    17161 atttttaccg atggctttat acggccttga cccgcgccac cgaaaatgtt tttctggtaa
    17221 attttcccat gaaatttttc tcgtaaaaaa cagagaattt ttttgcattt tccaactttt
    17281 aattactttt gtgaagaata taaaaattat aaaatctttt aaatttattc taaatttcat
    17341 ttttgctttt gaggctgctg tactgtttgt ttgcgctctt tgtcaacatt ctaaacattt
    17401 ttttgattta atttttccct gttagctgtt tattatttga ttttactttt tcaattgtgt
    17461 aaccttaata ttcaattaaa atgaaatctt cagcttccca ttttcttgtg gcaatctttt
    17521 taagcgtttt gattttgccc tcatgcatga acgatgtgga tcttaccaac atctccgata
    17581 aggttcaatg gaagggttct ttggctgtac ctattggaga agccgctctg acattggaag
    17641 atttgctggc tcaaatcgac agtcagaata ttcttgttac cgaaagtcaa gaaattcttt
    17701 atcaggattc cgacagtata gtttcccgtt tcagggatgt tgatttagct cagtattccc
    17761 aatcactggt aaaatcttac tatgtttctc caatagttag aactattcct gctaacacaa
    17821 ttttcccaac tttaaatgat aatgtaatta tcgatttggg cataaacaac tctccttccg
    17881 ctgaaagagt tgacagtgca gttatctatt cggccaattt gtcggtgaag gttaatgtgg
    17941 aaaatatcaa cattaatccc gataatctga aactgacgct ggagttcccc actgacaagg
    18001 tgcgtaccaa gaacggctcc ccgataaaaa ttgaatttac acctggagct tttaaccatc
    18061 ctgaaaatgt aaccatcaga gattttttga ttaatacctc tggaaatgcg tcgggaatac
    18121 cggtaacttt aaagttggat attaccaccg gcagttctcc cgttactgta ggaccaagtt
    18181 cggtagttgg cgtagaactg aaatttaccg gttttaacta taaggttgct tacggttatt
    18241 ttacaccggc agtaattgca accaaaatac tgacacaaga tttggattta ccttctttca
    18301 cggatgatgg ctcgtttttg tttgccaatc ccaaagtttt tcttaccgtt aaaagcaata
    18361 tcggggctta cctgaaattt aatgtggatt atgtgaaagc ctatcccaaa tccgattctt
    18421 ctcaagtggt ttatgcatct ttcaatggaa gtcgttcaac agccgaatcg ttggataaca
    18481 agccggcagt tccgggttta tggattgcta aaaaacttag aactttagac aaggattttg
    18541 gtgctaccga tttgcttttc agtaatgaaa acaaacccga tgtattggaa tataaataca
    18601 gcgttgacat tgacggaagc aaaaatgatg gtaaacctga ttttataacg cccgacggta
    18661 acattaaagt tttgtataag attcagattc cattatatct caaaccgggc agctattacg
    18721 aatttaagga caccattccc gatgccggtg aagatattaa ttcggctttg gaggatgtag
    18781 atgttaatag tggatcgatt ttcctcaccg ttactaacgg acttcccgta aaaggcaaat
    18841 taacgttggt tttgaaggat tcattgggaa gagatatttc tactaccttt aaaaaagaat
    18901 atgatattga tgctccgcaa gtagatgctg aaggttttgt ggtggaaagc agcattaccc
    18961 ctcaaaaaat agaaatcgcc ttaaacaacg atcaactgaa tgacttcaaa cgttcaaaag
    19021 atattgttta taaattcaga atcgacagca aagatgccaa ttcctttatt catttcagaa
    19081 aatcggatac cttcaaggta aaacttggtt tgtttgtaaa tggaaaagtt agttatgatt
    19141 caaataacaa cgattaataa aaccgaataa ccatgaaaaa gattattatt tcaatactga
    19201 caggaatatg ttgcttgacc tgcatctttt ctacatcagc tcagcaggtg aattcgttat
    19261 attttatgga aaattcgccc atccgttctt atttgaatcc ggcttttcaa ccaaccggta
    19321 gtttttattt gggattaccc gtgttgggtt atacttattt gagtttggga aacaattcgt
    19381 tgacgctgaa ggatttggtt tacaataaaa atggagagac catctggttt ttaaatccgg
    19441 aaggtgatgt cgataaattt tataaatctt tgcgttcctc gacgttgctc aataccaacg
    19501 aacaactgaa tcttttaaac tttggctttc gtgccggcag tgcttattgg acattcggaa
    19561 ttaccgagaa agtagaagga aatttggcat tgcctaagga tatgtttaaa ctattgcttt
    19621 atggaacacc cgaacaagaa aacaattatt tcaatttcaa ggatttgaat gccaatgtta
    19681 tggcttacac cgaatttgcg ctggggtatt ccaaaataat taatgaccaa tggacagtgg
    19741 gcggaaaatt gaaatttttg gccgggaatg ccattgtaac ttcttccaac gattatttta
    19801 atttgaatgc cggaatcgaa cagtggaata tgaaaggaac aggttctttg aatattgctg
    19861 ttcccggaga agtaacgatt ggcgataaat ttgaatctat cgattatact gaaccggaag
    19921 atatgcagag ttggctgaag ccttcgggta tgggtttagg gttcgacttg ggatttacat
    19981 ataaaccttt ggaaaatgta actctttccg catccttaat tgacatagga acgattaagt
    20041 ggaacaaaaa catgaaaaag gtagcctaca atatggatta tacctttgat ggacttgcaa
    20101 aaatagacaa tttggattct gtagatatgg atgcattttt ggatacgttg tccaatgcct
    20161 ttaaaaattc cttcacaacc gaagaaaaag caacggcatt ctctactgct acttctccaa
    20221 aattcaattt aggtgcagaa tatgcttttg caaacaacaa actcagtctg ggtttacttt
    20281 ctactaccac caaagtgaaa agcaacatgt atcaggagct gacagctgcg ttcaatatgc
    20341 gtccaagtaa ttggttcaac ttatctcttg cctattctct gttgaatggg agaatgagca
    20401 atattggagc ggctttggga ataagaaccg gatttttcta ttggaatctt tccagcgact
    20461 atgtttcgtt agataatgca cctttgttat tgagcgatat tgattcttcc ttgccatcaa
    20521 ccaaaattcc aattccttac aacagtaaag ggctgaattt ggcattgacg gttaattttg
    20581 tttttggtaa caaagtcgat aaagatcatg atggagtggt ggacaaaaag gataaatgtc
    20641 ccgacacacc caaagcagca cgcaagaaag tggatgcaaa cggctgtccg ctggatacgg
    20701 atcaggatag tattcccgat tatctggatc aatgtcccga tacgccattg gctgctcgtg
    20761 gttttgtgga tagcaagggt tgccttttgg ataccgataa cgacagcatt cccgattatc
    20821 tcgacaaatg tcccgacaca ccattgggag tagccgtaga ttctttgggt tgtccattgg
    20881 atacggacaa ggatagtgtt cccgattacc ttgatcggtg tcccgataca ccacttgaag
    20941 ctcacgggtt tattgatgaa aatggttgct tattggatac ggatcaggat ggagttcccg
    21001 attatctgga tttatgtccg aatacaccat tggaagcaat aaatttcatt gataaaaatg
    21061 gctgtccaat ggatagcgat agcgatggcg ttcccgatta tttggaccag tcacctaata
    21121 ctcctctaga agctcgagga ttggtagatg aaaaaggtgc tccccgcgat accgatggtg
    21181 atggcgttta tgatttcaag gatgattgtc caaaaatccc cggcgacagc accaatcatg
    21241 gttgtcctga agtgaaaaaa gaggtgcgca cacttttcca aagagcatta aaaggcatcc
    21301 agtttgatac cggaaaagat attatcaaac cttcttctta tcccattttg gaccaaatcg
    21361 ctcaagtatt gaaagaaaat ccgacctatt tggtagaaat tcagggacat accgataatg
    21421 taggtaatcc tcagaaaaat atggaacttt cagaaaaacg ggctatagcc gtgaaaaaat
    21481 atcttgtttc taaaggtgtg gaagaaaaca ggataacggc aaaaggctat ggagacacca
    21541 tgcccgttgc gtccaacaaa actgctgcag gcagagcatt aaacagaaga gtagagttta
    21601 ttgtaacttt tgaggaagtg aaattcgaat aaggaatatt tttcttttat agttgttcga
    21661 ttatttttaa ggcatttcaa tcgtaaagtt tgaagtgcct taaaattttt aggcaaataa
    21721 gtttttaata tcctgacgcc agtacatcgg ccagatgaat gccacaaatg gataaattat
    21781 gttttttgca gtaagtattg atattcataa ggcatgaggc ttccgtgctc acaataaatt
    21841 cggcttctgt tctaagactg ttggcaactt ttcgtgctgt catggaagtg gaaatggcat
    21901 gattttttac ggcaaatgtg cctccaaaac cacaacacgt atcattttcg tccatttcaa
    21961 ccaattgcag tccttctacc tgtttgagca gttttcgggg ctcatccttc aatccatatt
    22021 cacgtaaggc cgagcaagca tcatgaaaag ttattttgtg agggaaaaca gcgcctaaat
    22081 cttctgtttt taaaatattt accagaaaat cggttaattc gtacaactga cttttgattt
    22141 ctttaaattt ttgcagtagt tcggggttat tcgcaagtaa tttgttataa tgatgcaaaa
    22201 taaatccggt gcaggagccg gaaggagaaa ctataggcat atccgaatta aaatcgcaaa
    22261 gaaatttttc tgccaaataa gctgtttctt tccaataacc actgttgaat gcaggttgtc
    22321 cacaacaggt ttggttggaa ttgtagtgaa ctcgacaacc gactttctca agaattttta
    22381 taacgttaaa acctgtttgc ggataaagtt gatcaatata acaaggaatg aataattgaa
    22441 catccatgta tgtaaaaatt tagaaaaaat tttttgaatc agtaccataa atcgacataa
    22501 acaggaagat ggtcggaaaa accaccctga tacttcatgc ctacgtaagt tcggaaaggc
    22561 tgttttccta aaaatgtccg gtcatcttcc agtaaaaaat cggcttcaaa aatgtgcgca
    22621 tcacttggaa gagtataaac cgaattggtt ggggtcaaca aatttcctga aacaatgatt
    22681 tggtcgagtg tcccccattc tccattgtgt ttgtgcgagc cttttccttt caaatgcatc
    22741 gggaacatta aattgtacaa atttttttgt aaaaacggct ccgttggagg cagagctttc
    22801 agcgtttccg acagacttcg atttggcggg taatcgttga aatctcccat gataacaata
    22861 tttgctttat tgttggacga aaacaaactg tctacttttg aacgcaatac ggaagctaca
    22921 aatattcttc tttcttccga ttccaattcg cctcccagcc tcgaaggaaa atggcacaca
    22981 aagacatgta gtgtatctcc cgaaggaatt attcctgaag caaacaaaat atcgcgtgtt
    23041 ttaatttcgg gaaattgcgg aaagtttatg cgtatggctt catcgtgaaa aggtttaaat
    23101 acgtaaggtt ggtaaagaag tgcaacatcc acgcctctgg catcaggcga ctcgtgatgg
    23161 aggaatttgt attttaaatt tttcaggccg gcataacggg tcaagtctac cagacatttt
    23221 tcgctttcca cttcgcatag tccaactaat gccggacttt cccagccacc aatggcgcag
    23281 attactttac cgatgttggc ttgtttcttt ttatattttg ttgtattcca tgctcttatt
    23341 ccatttggca ggtattcttg gtcgtcagta agcgaatcgt ccacgcagtc gaaataattt
    23401 tcaacattgt aagaaacaat cttaaagttt cgtttctctt gacaataaag tcctacagca
    23461 aaaagaaaac aaattgttgt tgctaaaaaa actctttttt taccggaggt aatcgaggac
    23521 ttcatggtga agatgtttct ttgaaaattt tttaaccttg aataatttat tcatcctttt
    23581 tcgccggcat ccattcgtaa gcgcctgcat cgggtgctcc atcctgcaaa cggttaattc
    23641 catttaaatc gacaggacag ttttcggcag caatattgta atcggccaag cctcttaaag
    23701 gagaaacaga atccggttcg aaattgaaat aagttccttt tttgtaatca aaataaatat
    23761 ttttaaaaac ggtatcgttt ttctctgacc agcggatatt ggaaaattgg ggcaagtcta
    23821 aggcattggg cctacgaatg taacaatggg aaaaagttcc tttgtacaat ttagggaaac
    23881 gggtggcaag agtaaattcg ttttccatac tgccggctat tgcacaattt aaaaaagtag
    23941 tccgcatggg tgcaatgcgg tttaaatcca taatcataac ggccggttct ttgtcgcgca
    24001 gaattggctg aactgaactg ttgttaaaat aatttgctat cgtgcaatga atgaaagtat
    24061 gctgtccacc gttgagataa acgcaaaaac tgctgctgtt ggaaatttct gagttcaaaa
    24121 ccttcacatt gccgttttga accaccagtc cgtaaagcaa aaagttgtgt atccgacagt
    24181 ttgaaatctc aagttccggt aattgatttt tatcctgatt ggaaaaataa attccaacgt
    24241 agccactatt taaatttacg ttgtgcagaa catgttttcc tttattccaa agcaaataaa
    24301 ttcctcccca ttgcccggca acgacattgt acaatacggg cgtttcaaat tttattttgt
    24361 caaacctatc tccacgcatg gtgattggct tttcaaaagt tccgtcagcc attaaatttc
    24421 cataaacaat aagattggca ttgttgtgaa aatatatttt tactcccgga tccagcctca
    24481 gtgtttttgc actgtcgacg gcgaggtatc cgaaaacgag ataaggcttt tcggctgtca
    24541 gtgtcgtgtc gtttgcaatg agtttatttc tgaaaatgat tgcatcttgt ccaatggcct
    24601 gcaaggtaat tgtttttata ttgccattta ccagaaaaat taatgcatct tctatcagta
    24661 agggcaaatt gttgtcaagt ggatcgacgt tgacttgaac aaaaatatac aggctgtctt
    24721 ttgcgccaat ttctatatcg gcaaactgat tgttttcatt gtttttacca tctacattga
    24781 ttttaaaggc cgatgattta ccgcttgcca aaaaaatgct cgaaatccgt atggctttgt
    24841 tattgggatt gtaaaccata attctggaag ttgcactacc aatggatgta aaggtggtgt
    24901 cgaatgtcaa tgtgtccgtc gaaaatagaa cagtatctgc gggattggta gtaaaatctt
    24961 cctggcatga agagaaaaaa ttaattattg cccaaagaat aatcagtttt gtgaagaaga
    25021 aaactgtttt tttaaatttg aacattctat cgttggtttt tgctgtttta aatggatgtt
    25081 aagctggatt tgtttttgca aatttaatgt aaaagatgac tttatcataa tattggttta
    25141 tatttgtgca acaaaaaact taaatcattc tatcatggca agcagaaaag aattgaaaaa
    25201 acaaataaat gatcttacgg gtcagttggc attggaaatt ttatttcgtg gcactgttat
    25261 gcaggttttg gaacctgaaa aaatgtatgc tttgctatca aaagttatga tcatgaacag
    25321 gcagtttatt tcaagggtaa atgcagcaaa aaggataaaa agttcaaaag aagtgaaaga
    25381 tttctatcaa caatttttta ccgattggga aaagaatgtg ggagaaatta ttcaagagtt
    25441 ctaatttttc tttcagcggt tatgcactct gacattttga aacttactcg aaattaaaag
    25501 aaagggttat cattttattg gtaagtttgg aaatggcagt ggaataaatt ttatcttctt
    25561 cggttaatcc tcctgcattt ctttgttcga gaggcgtaag catgttgttg aatccaatgg
    25621 caaattttaa ttcgggtgcc aatttgaaaa aactgaaata taaatcacat cctacgccaa
    25681 attcaacaaa ataatccatc ggttttagta agatgggttt ttccttgttt cttccaaaat
    25741 caaaatacac tcctccgcct cccaacagat agggacgata attgtctttc ctttcagcac
    25801 tgtattttaa atatacgggg aagctcagtg gaatagagtt gacagttgtg gagccagttg
    25861 tacttgtggt atctttccga taagtgatgt ctctttcgga aaaatgtaaa gttggattga
    25921 agcgcagatt ccaatatcgt gccaatcgaa gattggttat cacaccaacc gaaaatccgg
    25981 gttgcaaggc agatacatct gtgtaatacc tgctcccatt aataagtgta tctcttggcg
    26041 ttattccaaa atcagccgta ttcaatccta aagaaaaccc gaaatgaatg agtttgtcat
    26101 ctacgtaagg taaatttcct tgagctttaa ctttaggaaa aaacaccgaa caaaaagtta
    26161 agataaaaat aaaagcaaaa atattgtttt ttaacatact gatttcctaa gaattttcac
    26221 gttgggcttt gctataaaaa acgcttaaaa gttgatatta ttgccggttt aacgagcgag
    26281 gattgagaac aaataataaa gaagaaaagg caagaaattt tacaaattgg aaaaatagta
    26341 ataatagaca atgatttgac tgagtgtcaa accatcaaat ttccgaattc ttcgtccata
    26401 gctgtcatat aaagcatttc cggaaactgt agccaacttt gttccgagtg catcataaaa
    26461 atcgttattt tcgactttcc caatttgttt cccgttgcca tcgtaaagat aacttccgtc
    26521 gattttccct atcaggtttt ctgctgcgtc ataaacaaaa acgctttcaa cttttccttt
    26581 caattttgaa ttattgtcgt aataattttg tccaataatt tttccaatag gcttccctgc
    26641 agcattgtag atttgttgtc ctattaatgc agaatttgaa aaaaggaaca gaagaaatag
    26701 tatctttttc atctcaaaag acttttagtg aatacaaatt aattgaaaat aaattgaaaa
    26761 aacaaataat gagtcatttt ttattctgct tcagaaaaaa tttgaattta ggtgttgttt
    26821 tattcaaagt tgttttttca atgggagtta gatactacac tgaaatttta gttgttaatt
    26881 gagatatttg atgtaaattt gcattaaatg cagtgtaaac cgacaacaaa ttaatttttt
    26941 tgagtttata attttttatg aatcgactgg atgcggtttt ttttgcttcc gatgcgatta
    27001 cggttgcccg tcaactattg ggaaaaactt tagtaagaaa gtgggaagat ggtacaattt
    27061 cccgatacaa aataacagaa acggaagctt attttggaga agaagataag gcttgtcatg
    27121 cctgcaaggg aaaaactcag cgaacacaga ttatgtatgc cgaaggaggt tacatttatg
    27181 tttatctgat ttatgggatt tattggttgc tgaatgtggt tacaggcaac aaagaacatc
    27241 cggaagcagt actgataagg ggaattaaca atatttcggg atcaggaaga gtgggaaaag
    27301 aattaaaact tgataaaagt ttttatggcg aaaatctaat ctcttcagat agaatctgga
    27361 ttgaagatgc tcccgaagcg ttatattttt ttacggctac gcgcatcggg atcgattatg
    27421 ccgatgaaga atggaaatca aagccatggc ggtttgtaat gacatcatcc gaaaaataaa
    27481 atttcttttc cccggtgatt tgataatgga gatttggaac aactcaatta ttgtaaagag
    27541 atctgttatt ataattttta agaaggtttt tatttgatat gttttgcaac ttaaaatttt
    27601 ttttaccttt tctgactatt ctttggcgga ttggttcagt gaacgcaaaa cacgatcgat
    27661 aatggatgtt tcaaaacctc gactttgggc aaatcgaaaa agtttacctt gtttttcata
    27721 ttcgtaatcg tatttgagca ttgatagttt tgacttaagt gctgaagcaa gcatttcctg
    27781 atattctcca tcatcaatgg tagaaattgc agattttatt tcatcgttag atattccttt
    27841 tgatttcaaa gccaattcaa tcttgatttt tccccattta tttaatcgaa atttatcccg
    27901 aacaaacgat tgggcataac gtttttcatt gataaaattt tcttttatta atctatcaat
    27961 tatttttctt ttttcatttt ctgaaacgtt ccaatggctt agtttttctt ccacttcaga
    28021 aacacaatgt tcagaaattg aacaatagga tgctgctttt aaaaaaagct catcggtggt
    28081 atacgttttc ttcatgataa aaagattttt ttgtaaatga taaaggaata caaaaataat
    28141 aattttcaaa aaaatcggac tgttttcatg gttttttcta tatcagacga aagggtattt
    28201 tcaaaaataa atgaaggaaa acaagcacat aatttgtaaa tttgcattcc taaaaattta
    28261 aaaatggata caaaaggagc acccataaca ttaggaacag aaaaaattgg aaaattactt
    28321 atccaatatt ctttgcctgc catcattgcg atgactgcat catcgctcta caacataacc
    28381 gacagtattt tcattggaca tggtgtaggt gcaatggcta tatcgggatt ggcagtaact
    28441 ttcccgttta tgaatttgtc ggcggctttc ggatcgctgg taggcgtcgg agctgcaaca
    28501 ctcatgtcgg taaggttggg acaaaaggaa tatggcacag caaacagaat tttgggaaat
    28561 gtctttgtgt taaatgttat ttttggagtg gcgttcatga cggtttcttt aatttttctc
    28621 gatcctattc tgaaattttt tggcgctagc gatacaactt tgccttatgc acacgatttt
    28681 atggtagtca tacttatagg gaatgtattt acacatattt atcttgggtt aaacggtatt
    28741 ttgcgctcga taggcaaacc gggaaaggcc atgtatgcga ctttgctgac ggtattgata
    28801 aatttgattt taaatccgtt atttatcttt gttttaaaat ggggtatcaa aggaagcgct
    28861 ctggcgacag ttatttcgca aatagtaatg cttgcttggc aaattcgtca tttcagcaac
    28921 aagaataatt ttgttcattt gcaaaaggga attttccggt tggataaaaa aattgtactc
    28981 gattcgttaa ccattggctt atctcctttc ataatgaatg ctgttgccag cgtgattgta
    29041 attgttatca atcagacctt gatacgctat ggtggcgatt tggctattgg ggcgtatgga
    29101 attattaatc gggtcagttt tctttttgtc atgattgtga tgggactgaa tcagggaatg
    29161 caaccgatag ccggatataa ttttggagct aaaaatttta atagagtaaa tgaagtttta
    29221 aagctcagca ttttcatagc tatgggaatg atgactctcg gcttcattat tgtagagttt
    29281 tttcctcatg ctgtggcttc tcttttcaca agcgacaaaa ctcttatcca attgtcgata
    29341 gaaggattga gaatcgtatt tgttttgtat ccgatggtag gttttcaaat ggttatcacc
    29401 aactttttcc aaagtatcgg aatgtcaggc aaagccattt tcctgtcact tacccgacaa
    29461 ttgttgttct tgttgccttt tctgctcgta ttaccaactt tttacggatt aaaaggagtc
    29521 tggtatagta tgccggctgc tgattttctg gctaccatcg ttgcggctta tatgcttttt
    29581 gtgaatttcg gaagctttat taagaatcaa aatagagatc acaagtaaaa aataactttg
    29641 tgattaaact
//
LOCUS       DAOSIB010000020        28347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_1864_length_28347_cov_12.570197, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000020 DAOSIB010000000
VERSION     DAOSIB010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 28347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28347
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1864_length_28347_cov_12.570197"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3378
                     /locus_tag="PLD44_03215"
     CDS             <1..3378
                     /locus_tag="PLD44_03215"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOF98381.1"
                     /translation="TKKPFVLPKELNQLGMVRYNGNISGFLNNLVAYGKLRSNVGTIA
                     TDILLQYNTKSQYLAYNGTIKSDNIQLNKILSNNQFGLFSFNFNTKGEKQANKNFQGT
                     IEADIPEFQWNGYSYRDMQLKGNYDGSGFDGTFDVADENIDAHFTGIVDMTKRLPVFD
                     FSLNLAKANLNALKLTTKYSNANLSFNARTNMIGNSPDNLNGFVLFDKIAFTNRNRTL
                     NAGKIQFVSRTENNETYFLISSDYVNGSISGNFIYSSLDQTFKRLAYHYLPALSNKIP
                     HENSHPNFINVDLKISDTGELTDILELPFQLKGNSYIKGYYDEKIYKVDLISNFPYFK
                     FGKREFENIFLSLQNEEDKLSFITRAQMDGKNKPMHFYINATASDNSVNTLLGWQNSD
                     SITNAGEFKTLTKFAATDGKINAQISVLPSQIILMDSVLNIFPSVVKYNSDGSLQVNN
                     FKMEGSSQFLHVNGIASKSEKDSLQFAMNNLDLYFIMNDLVKLKDPSLGGYFTGNATM
                     YSAFQQPFFEAKLLVKDFSMNQSVLGDVNLYSYWDKEKKEVVSNAHVLNNKDTVAYID
                     GVYVPGKDSIDFVYDMHHLNMGMLKRYFGDVVQNVRGFATGKLRMFGPTKKMRFDGDL
                     YVAEGQLKMALTQTTYFFNDTIRMRPTSVEFNNIKMYDEEGNEGLLDGIMKHTGMFGD
                     LKYDVMIRSNNILALNTHANANEIFYGKAYAGGTVHVFGDVNVTNVWVDMVSRPGSKC
                     YVTMPGSSTAADNSFVLFVNKNDTIKTQPDEQKGKKLPENTYNTKVNLQIGITPDAEI
                     QFIIDPKAGDMLVGKGTGNLRFEFDTYSDIKLFGTYTIEKGNYLFTLQNVIRKEFKID
                     EGSTLTWTGDLYNAQVKIKGIYSLMASLRDLMDESQLSSLPRTTVPVNCILLLSGNLM
                     KPNVEFDIDLPSSDESVKQMVKSIISTNEMMARQIIYLLIFNKFYNPYLQWAANAPVG
                     IGSSEAVSLVVSTLSAQMNNWLAQMINSNIISIGFDYRQTDQLSSDVQAQFLIQPNNR
                     LIINGNLGYRNDNVNLSNNTNKFIGDIDVEWLLTEGGKFRLKAYNHTIDRYQLRSAKN
                     TQGIGLIYKENFSNFDELFRYYWNILTFKKKNNEADQ"
     gene            3362..4012
                     /locus_tag="PLD44_03220"
     CDS             3362..4012
                     /locus_tag="PLD44_03220"
                     /inference="COORDINATES: protein motif:HMM:NF023074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3256 family protein"
                     /protein_id="HOF98382.1"
                     /translation="MKLINNFFTVIFFFLCAKISAQGIDKLYVGMPDALHPTLTSQQR
                     LELIEYAKAGQNDTTLNRYKKPVFLLKLDTLHQHLMVKNSGSTSFEMKVLDANSNEPM
                     IAIIRTVCAPICHSLIEFYDFKWNRLPVTFHYPQATDWLDLEKAQKTEEGVETFQKIL
                     KYTFVSLSFQEEGQIVMAFNNSIDFLSDEDKKLVFPCMNTAPLLFQWSDLKWIREK"
     gene            complement(4070..4339)
                     /locus_tag="PLD44_03225"
     CDS             complement(4070..4339)
                     /locus_tag="PLD44_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOF98383.1"
                     /translation="MKSGIHPTNYRPVVFKDLSNGDTFITRSTINTKETIEIDGVTYP
                     LVKLEISSSSHPYFTGKAKLVDSAGRVDKFMNRYAKSLEKKKTQK"
     gene            complement(4461..5063)
                     /locus_tag="PLD44_03230"
     CDS             complement(4461..5063)
                     /locus_tag="PLD44_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOF98384.1"
                     /translation="MSKVLYIKANAKPEGESRTFMIADKFVEAYKKHHPQDEIITLDL
                     YKEDIHFLTVEDVMIHMSNEVEETENAMKKYALQFKSADKYIFAEPMWNLGVPAILKA
                     YIDYVSIPNITFKYGENGPIGLCQGKKALNVITSGGDYSEGMAAEWDLCDKYLKTIMG
                     LFGVLDYTTIRASNLDVIGMDVDAIVNKAIKEAEELAKTF"
     gene            5396..5740
                     /gene="rpsF"
                     /locus_tag="PLD44_03235"
     CDS             5396..5740
                     /gene="rpsF"
                     /locus_tag="PLD44_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOF98385.1"
                     /translation="MNHYETVFILTPVLSDLQMKEAVEKFKTILQENGATITNEENWG
                     LRKLAYPIEKKSSGFYCLLQFDAEPSVVAVLETNYRRDERVIRYLTTRLDKYAYEYAE
                     KRKNVKSKEGKE"
     gene            5744..6016
                     /gene="rpsR"
                     /locus_tag="PLD44_03240"
     CDS             5744..6016
                     /gene="rpsR"
                     /locus_tag="PLD44_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOF98386.1"
                     /translation="MATNNNEEIRYLTPPTVDIKKKKYCRFKKSGIKYIDYKDPEFLK
                     KFVNEQGKILPRRITGTSLKYQRRVAQAIKRARHLALLPYVTDMLK"
     gene            6037..6480
                     /gene="rplI"
                     /locus_tag="PLD44_03245"
     CDS             6037..6480
                     /gene="rplI"
                     /locus_tag="PLD44_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOF98387.1"
                     /translation="MEVILKEDVNNLGYKGDIVKVKDGYARNYLIPTKKAVIATDSAK
                     KMLEEEKKQRAHKLERIKNEALELAEKAKSINLVIEAKASSTGKIFGAVGPIQIADAY
                     EKAGLVIDRKVIVVDEPVKELGSYKATLKLHREVSVEISFEVIGV"
     gene            complement(7237..7950)
                     /locus_tag="PLD44_03250"
     CDS             complement(7237..7950)
                     /locus_tag="PLD44_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOF98388.1"
                     /translation="MKIDVCFSPALYPFYSSGNSKIAVIVDVFRASTTICKALMEGAS
                     AIIPVASLEKAEELKSRGYFVGAERNVEKCDFADAGNSPFDYYSEKVKGKEIVFTTTN
                     GTRAIELSAAENEVIIGAFCNINAVADFCRNAGKDVLVVCAGWNNLFSLEDTLFAGLL
                     VHSLKSSGNFETSGDSAVAALVLWQQAEPDIKTFLQQSEHAKRLFTHHLEKDFDFCLA
                     QDTLPMVPFYDKSSQKILF"
     gene            complement(7997..9397)
                     /locus_tag="PLD44_03255"
     CDS             complement(7997..9397)
                     /locus_tag="PLD44_03255"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HOF98389.1"
                     /translation="MEKTSEEKKSFQWKDVFQKIISDLKTLTNLHEDTNIESTIASIK
                     KDVEFKGANVWILFFAVIICSVGLNVNSTAVIIGAMLISPLMGPINGVGLSIGIYDTE
                     LLRLSLRNLLIMILVGLVASTFYFLITPITDAQSELLARTRPTLFDVFIAFFGGCAGI
                     VAASRKEGKITVISGVAIATALMPPLCTAGYGIATGQLRFFFGAFYLLFINSFFIAIA
                     TFLVTHLLHFPVKQFVDIQRQKVVKRSIIIFSILTIIPSFFLAWNVVSETRFNSHTYR
                     YIQDIQQLDLFSEKEIINVSRKYSRKEKSLTLSVIGKPLTEEQIRYIQQLLPKYGLNN
                     VNLIIKQTGGTFDLGAQTAFIEKLLMDKDIQLQKKDSIIAALNNKLQLMQTSGVNELQ
                     LAREVKVQFPDIKQIAFSNSIFINLDHLTQDTVPTIFIQWEETPPNKKALLSQMNEWL
                     KVRLNLEKLKIIEVEM"
     gene            complement(9456..11615)
                     /locus_tag="PLD44_03260"
     CDS             complement(9456..11615)
                     /locus_tag="PLD44_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOF98390.1"
                     /translation="MVAFEKYFVKKKRTLVFLSIFFLFTGAKADEGMWMLPYIEQLNI
                     QKMNGMGCSLSAEDIYSDKNISLKDAVIVFGNGCTGVVVSPQGLIFTNHHCGFDAIQQ
                     HSSVEHNYLKDGFNAVRLEDEIPTPGLSVKFLVKIEDVTNQVLSHLPDSLSEKSRNEK
                     IYEISDSICKAAEKDTHYLAEVKPFFAGNEFYLLVYEEFKDVRLAFAPPSSIGNFGGD
                     TDNWMWPRHTGDFSVFRVYATPDGKPAEYSKDNVPYSPKRFASVSNKGYQTNDFTMIL
                     GNPGSTSRYLTSFGVKFRMETGNQARIDVRGIKQTIWRSFMRKDEAINIAYANKYASS
                     SNYWKNSIGMNKAIQKLNVLERKREQEAAFLEWTKSNPEREKKYKDVLKTLEDGYSTL
                     YPYARALNYLRESLMSGVELPRIAQKASQLISQNLPKDSLIKELEEIYKDYRPEVDQA
                     VFAAMLDVYKNSVDADALPAFYQEIQKKFKGNTALYAASIFQNSAFSSVDKLKKNLQK
                     KKIKIANDPALIFGNEVRNTYEQISSGIYAQTSEKVKEAERIYFSGLREMYEEQGKKL
                     YPDANFTMRMTYGKIGGYQPADAVDYHYYTTTKGILEKENPTDPEFIVPDKLKKSILE
                     KDFGKYLDPQTKDMHVNFISNNDITGGNSGSPVFNGKGELIGLAFDGNWEAMSGDIVF
                     EPELQRTISVDIRYVLYVMDKIGGAQRLIDELSISGY"
     gene            complement(11810..12826)
                     /locus_tag="PLD44_03265"
     CDS             complement(11810..12826)
                     /locus_tag="PLD44_03265"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOF98391.1"
                     /translation="MELDWNNIGFGYVKTDYNIRCSYQNGEWGEFEVHEDEYLTLHMA
                     ATCLHYGQEVFEGLKAFRGIDGKIRVFRMDENAKRMQSSCKGIVMQPFPVDRFQEAVL
                     KVVKLNERFVPPYESGASLYIRPLMIGTSAQVGVKPSREYLFILFVTPVGPYFKGGFQ
                     TTPYVILREFDRAAPLGTGIYKVGGNYAAGLAADEKAHSLGYSAVFYLDAKEKKYIDE
                     CGAANFFGIKNNTYVTPKSTSVLPSITNKSLMTLARDMGMKVEQRPVPIEELSTFEEA
                     GACGTAAVISPIQRIDDLDENKSYVFSPDGKPGVICEKLYKKIRAIQYGEEPDIYGWT
                     TIVE"
     gene            complement(12891..13499)
                     /locus_tag="PLD44_03270"
     CDS             complement(12891..13499)
                     /locus_tag="PLD44_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOF98392.1"
                     /translation="MLSMSYLSPIDYFMTLTNARSAVIEQHEYYQKQTYRNRCRIAGA
                     NGVLDLIIPVEKTANNKATVRDIRISGHNDWQRQHWKAISSAYLSSPFFEYYLDDFRP
                     FYEKNWLFLWDFNMEIQNKIFELLEIYPKLEFTDKFIDKIEENILDLRLQFHPKKQPF
                     LKIPSYYQVFENKFGFISGLSIIDLLFNMGNESLLWLLGKAE"
     gene            complement(13591..14991)
                     /locus_tag="PLD44_03275"
     CDS             complement(13591..14991)
                     /locus_tag="PLD44_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOF98393.1"
                     /translation="MTSEPHTSRFHQPRRQWIKAAIWSLIYILFIAWVGNFWWLLLLP
                     VIFDMFITKFIPWTWWKKTKNKTLRSIYSWIDAIVFALVAVYFINIYLFQNYQIPTSS
                     LEKTLLVGDFLFVSKASYGPRIPNTPLSFPLVQNTFPIINTKSYLEKPQWEYKRLKGF
                     DTIAHNDIVVFNFPTGDTVCLKQQNPDYYQLCYYYGRDVVRSRKDIFGEIVYRPVDRR
                     ENYVKRCIGLPGDWLQIKSNQVYINGEKQKPFPGVQFNYLVQTDGTRLSDELLTKLGI
                     SKDDRLILNTSQNPQEIMALGLDPSFPIYHFPLTGEMLQKLQRVTGVVKIVIEPEMYG
                     GDVFPLGGGKNWTRDNYGPIYIPKKGAVLELNAYNYPIYERVIRTYENNKLEYKNGIF
                     YINDRPEKTYRFKMDYYWMMGDNRHNSADSRYWGFVPEDHIVGRPVVVWLSLDKDKSL
                     FGGKIRWNRFFKDARR"
     gene            complement(15092..15808)
                     /gene="dapB"
                     /locus_tag="PLD44_03280"
     CDS             complement(15092..15808)
                     /gene="dapB"
                     /locus_tag="PLD44_03280"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOF98394.1"
                     /translation="MKLALIGYGKMGKAIERIAKERGHEIVCIIDIDNLNDFESDKFK
                     SADVAIEFTHPGVALSNYRKTFAAGIPLVSGTTGWTNELPALKKEVEEKGYTFFWSSN
                     FSLGVNIFMAVNKYLASLMNRFSDYDVEITEIHHIHKLDAPSGTAITLAEDMLENLER
                     KTCWVKEEAKQPHELAIKSIREDEVPGIHIIRYDASFDAIEFKHEAKTRDGFALGAVM
                     AAEFAVGKKGWLTMQDLLKF"
     gene            complement(15873..16232)
                     /locus_tag="PLD44_03285"
     CDS             complement(15873..16232)
                     /locus_tag="PLD44_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOF98395.1"
                     /translation="MKKTLVIGASTNPERYAYKAAQRLLENGHEIELLGLRPGEIFGK
                     SIDTEKKQYPDIDTVTLYVGPKNQPEYYDYILSLHPKRVVFNPGTENPEFEALLEANG
                     IQAQEACTLVLLSTQQY"
     gene            complement(16292..17344)
                     /gene="aroB"
                     /locus_tag="PLD44_03290"
     CDS             complement(16292..17344)
                     /gene="aroB"
                     /locus_tag="PLD44_03290"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOF98396.1"
                     /translation="MTSSSTIICENFIAELNEFIEYQKPDGIFVLVDENTKKFCLPLL
                     LQSEKLKNCQVITIPSGDENKNIHTAIEVWNYLSSHGATRRSLMINAGGGMVSDLGGF
                     AAATFKRGISYINVATTLLGAVDAATGGKTGINFMGLKNEIGVFAPADKVLINVDFFR
                     TLDDKNLRSGFAEMIKHSLLDSREHWKNILKFNLNTIDYELLKHRVAESVKIKEQIVE
                     KDPKEQNLRKALNLGHTFGHAFESFSYSQHQPVLHGYAVMYGLVCELYLSHVKLNFPK
                     EDLLKLVTLAKENYGKISICCDDYEQLYELMTHDKKNVSDTINFTLLSEVGKIELNQT
                     ATKKEIFECLDFYQEM"
     gene            17526..17831
                     /locus_tag="PLD44_03295"
     CDS             17526..17831
                     /locus_tag="PLD44_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOF98397.1"
                     /translation="MEQINNINIDQMEEAAYKLRAISHATRLCIISLLSEKEELNVSQ
                     ISEELNCEQSLLSHHLTDMRAKGILNCRRDGKNCYYSLKNKEIVQILDCIRSCQCTN"
     gene            17845..18072
                     /locus_tag="PLD44_03300"
     CDS             17845..18072
                     /locus_tag="PLD44_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HOF98398.1"
                     /translation="MLKKFLSRHWLKVVGVLLGSIGGFAYFYFVGCKSGTCPISSNPY
                     ISIVYGAVLGYLFFDMFKVKSKNTNANDQED"
     gene            18384..18878
                     /gene="trxA"
                     /locus_tag="PLD44_03305"
     CDS             18384..18878
                     /gene="trxA"
                     /locus_tag="PLD44_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOF98399.1"
                     /translation="MKKLTGIFLMITFIALSACAGSNGENKQNNSQNKNEEKLMGTIH
                     LTKEEFLKKVVNYEASPNEWKYLGDKPCVIDFYADWCGPCKAVAPILEELAKEYDGQI
                     YIYKIDTDKEQELAAAFGIRSIPTLLFCPMNENPQMAQGALPKDVLKNAIDEVLLKKE
                     KVKQ"
     gene            18894..19292
                     /locus_tag="PLD44_03310"
     CDS             18894..19292
                     /locus_tag="PLD44_03310"
                     /inference="COORDINATES: protein motif:HMM:NF015581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HOF98400.1"
                     /translation="MEEFFLEKKSKFSFHKTLSELTNAIQEGGWRLVYVHDLQETMAR
                     NNKEVLPVKVLELCSPYHAYSILGKDEWRTFSSMMPCRISIYEKSDGNTYVSQMNISM
                     LAPLMEKEIQGIMNISYAEVQNFVSRIIEN"
     gene            complement(19489..20433)
                     /locus_tag="PLD44_03315"
     CDS             complement(19489..20433)
                     /locus_tag="PLD44_03315"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOF98401.1"
                     /translation="MKILVCPLNWGLGHAARCVPIIRQLITEGNDVVIAADGFPLEFL
                     QLEFPELRFLKAPTQNVRYSTGNSQIWAMFKSIPRIFGNIFQDHCWLHRLLKQEHFDR
                     IISDNRFGCWNKRTYSIYITHQLMVKMPSELRLLEPIVWRLHRFFIHRYDECWIPDLA
                     GDENFSGDLAHRYPLPENAHFIGILSRFQQLKNIIPNYTYETVMVLSGPEPQRTIFEK
                     QLTEKFSKDTVKSLMIQGLPQEKQTSVQVGNLTKVSHLSTQEMASLLAGAKKIIARSG
                     YSTIMDLQALNCLDKAILIPTPGQTEQIYLAELHKKCR"
     gene            complement(20449..21219)
                     /locus_tag="PLD44_03320"
     CDS             complement(20449..21219)
                     /locus_tag="PLD44_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOF98402.1"
                     /translation="MILFDQIVFGPVHSRRLGTSLGINLLPVDAKICSFDCLYCECGF
                     NTTGKHAKLPTRMEVRNALEMRLKEMASDGQIPDIITFAGNGEPTLHPDFAGIIDDTL
                     ELRNKYCPNAKVGVLSNATQIHKPDVFNALKKVDDNILKFDSAFDHTLQLLDRPVRKY
                     ITVRWLIEHLKKFEGKMIVQTLFIRGEVNGEKIDNTSDKEIKAWLEALKEMNATQLMI
                     YTIDRETPLKTLEKIPLEELNRIAEKAKSYGFDVSVSG"
     gene            complement(21284..21931)
                     /locus_tag="PLD44_03325"
     CDS             complement(21284..21931)
                     /locus_tag="PLD44_03325"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HOF98403.1"
                     /translation="MTYEELIHDTEYKELASLSHNEIKNFIVNEITENQQWARAANLY
                     QLTGILAFMLGTFKAFMPYFVHRQAFGLLWLLAGIVFTFTILIVFHELIHALAYWAMG
                     ARHLFFGCQPKKFMFYVQADKAVVGYRQFKIIALAPTVFISFLSLLGMIIFYHQPAFY
                     FFISIFGLHSLFAAGDFGLLCFFQNRPELEIVTFDVKEEGKTYFYGKEKRKNIAG"
     gene            complement(21944..23899)
                     /gene="dnaG"
                     /locus_tag="PLD44_03330"
     CDS             complement(21944..23899)
                     /gene="dnaG"
                     /locus_tag="PLD44_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOF98404.1"
                     /translation="MIDQATIDRIMEAAQIQDVVSDYVTLKKRGVNMIGLCPFHNEKT
                     PSFTVSPAKGIFKCFGCGKGGNAVHFIMEHEQLSYYDALKFLARKYNIEVQERELTDE
                     EKELHNERESLLLVNEFAQKYFSDTLYHHIDGKTIGMSYFQERGIREDMIRKFQLGYC
                     LDQQDAFTQKALKAGYNKKYLVKTGLTIETENNRLTDRFRGRLIFPVHSLSGKIIAFG
                     GRVIKKDDKTAKYLNSPESEIYHKGNELYGMYFAKQAIVKEDRCLLVEGYTDVISLHQ
                     SGIENVVASSGTSLTPAQIRLIHRFTNNVTVLYDGDAAGIKASLRGIDLLLEEGLNIK
                     VVLLPEGEDPDSFSHKLNAVDLLNFIRDNSVDFIRFKTKLLMEDAANDPIKRSNLILD
                     IVRSIAIIPEPIVRAEYVKECSNLLQVNEEILYHEINRLKRKESEKKLGQSNVSASST
                     EPKPQPVSPQEMLNAENQRFEQEERNILQVLIKYGKSALYYSDEENRSPITVEDFIIE
                     DLEQDQLEFVHPLYAQMLQEYKEQCLKKNINVQNFFLHHIHPEISKLTAELISEKYTL
                     SKIYKKTRMDEPEIDKVREFAERAVLEYKNSIVLSQIKQKLLEMKIAAENHDDELVRI
                     LMIQISQLEEIKNQLAKNLGERIVIKL"
     gene            24146..24319
                     /locus_tag="PLD44_03335"
     CDS             24146..24319
                     /locus_tag="PLD44_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98405.1"
                     /translation="MKDFLPYLGSIIILIGVALLAYYQFGGHPSNLILGTAGILMIVG
                     FFTHLFLNKKIVE"
     gene            complement(24714..26483)
                     /locus_tag="PLD44_03340"
     CDS             complement(24714..26483)
                     /locus_tag="PLD44_03340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOF98406.1"
                     /translation="MKKSNNFFKTIYQKYPYLFVLFLSLLLTIPWTMVSDFYTKGEPR
                     EAAVAQCILNTGNWILPTDYADETAYKPPLMHWMIALFSLPAGKVSETTARLPSALGL
                     IGITVSFFVFLSKRTSKKRALLATLLMLTSFEMHRSGIEARVDMLLSFFMIASLICFY
                     IWEEKGLRKFPWFISLLLAGASLIKGPVGIVLPCLVFIVYLLLLQKYSFWKIIWKNIV
                     VALPAVFILLCWYYLAYRQGGEKFLNVVYAENFGRFLGQKSETLDIAYNLGHENPFWY
                     YIPAILTGFLPWSLVAIFSLFAVSYKKLFKKLQFRKLKESFKNLKNADKLVLFSTITL
                     IVVVGFYSIPSSKRSVYIMPVYPFAAYLLVFLYEWAMKQKPQFINWTANIIFILVGFL
                     LLLMAFSRFVNLSAITSPLIKDKTLNHDIGLYSKAFQHPGVAGVLFWLFLATAFVWCL
                     KWKRTRIVQNILFTCIGLFIGLQVFLEGFAYPIYKNGYSVRPFAEKLQNKYSLKNNVF
                     VMNNLKEYPNLYALNFYLGNRFKNFEKEKPKEGYFLTGEKSLKKIRKVYGTNYQFTVL
                     ENSGNRFNDIKDVILFCRLKRIK"
     gene            complement(26574..26903)
                     /locus_tag="PLD44_03345"
     CDS             complement(26574..26903)
                     /locus_tag="PLD44_03345"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOF98407.1"
                     /translation="MSATGKEFLKILRRIFLNKYTLVFLIFAGYVTFFDKHNLISRWK
                     IYQKNKELEKEIKYYDEKIKSDRTKLFELKSSNENLEKFAREHYFYKKDNEDVFIINK
                     KDSNKNR"
     gene            27245..28117
                     /gene="fabD"
                     /locus_tag="PLD44_03350"
     CDS             27245..28117
                     /gene="fabD"
                     /locus_tag="PLD44_03350"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOF98408.1"
                     /translation="MKAFVFPGQGAQFVGMGKDLYEQSTLAKEYFEKANEILGFRITD
                     LMFEGTPEDLRQTKVTQPAVFLHSVISAFVLGEEFNPDMVAGHSLGEFSALVAARALT
                     FEDGLTLVSKRAVAMQKACELEESTMAAIIGLDDEIVEEVCDSIKDMIVVPANYNCPG
                     QIVISGTLQGVDMACEMLKERGARRALKLSVGGAFHSPLMEPARLELSEAISQTTFSI
                     PICPVYQNVNAYPQTDPVNIKQNLIAQLTAPVRWTQTIKNMITDGADEFIELGPGDVL
                     KGLIKKINPNVIVH"
BASE COUNT         8844 a         5250 c         5227 g         9026 t
ORIGIN      
        1 acaaaaaaac catttgtgct gccaaaagag ttgaatcaac tgggaatggt cagatacaac
       61 ggcaatattt ccggatttct aaacaacctg gtagcctatg gtaaactgag atcgaatgtg
      121 ggtacgatag ctacggatat cctgctgcaa tacaatacca agtctcagta tttggcttac
      181 aatggcacca ttaagtcgga caacattcaa ttgaataaaa ttctgagcaa caatcagttt
      241 ggactttttt catttaattt taacaccaaa ggagaaaagc aggccaataa aaattttcag
      301 ggaactattg aagctgatat tcccgaattt caatggaatg gttattccta tcgcgatatg
      361 caattaaaag gaaactatga tggaagtgga tttgatggga cttttgatgt tgccgacgaa
      421 aacatcgatg ctcattttac tggaattgtc gatatgacaa aacgtttacc ggtattcgat
      481 ttcagcctga atttggccaa agccaatttg aatgcgttga aactgacaac taagtattcc
      541 aatgccaatt tgtccttcaa tgcaagaacc aatatgatag gaaattcacc ggacaatctc
      601 aacggattcg ttttattcga caagatagct ttcaccaacc gaaatcgaac actcaatgcc
      661 ggcaaaattc agtttgtttc tcgtacagaa aataacgaaa cctatttttt gatttcttcc
      721 gactacgtga acggaagtat ttcgggaaat tttatttaca gctcattaga tcaaactttt
      781 aaaagattgg catatcatta tttacctgcc ttgtcgaaca aaattccaca cgaaaattca
      841 catccaaatt ttatcaatgt tgatttgaaa atttctgata caggtgaact gaccgatatt
      901 ttggaacttc cctttcaatt gaaaggaaat tcgtatataa aaggatatta cgacgaaaaa
      961 atttataaag ttgatctaat ttcaaatttt ccttatttca aatttggaaa aagggaattt
     1021 gaaaacatat ttttatcact tcagaatgag gaagataaat tatcatttat tacccgcgca
     1081 caaatggatg gaaaaaacaa gcccatgcac ttttatatca atgcaacagc atcggacaat
     1141 tctgtcaata ctttgttggg ctggcaaaat tccgattcaa ttaccaatgc aggagaattt
     1201 aaaacactga caaaatttgc agcaaccgat ggaaaaatca atgcacaaat ctctgttctt
     1261 ccaagtcaga ttattcttat ggattccgtt ttaaatattt tcccatctgt tgtaaaatac
     1321 aattctgatg gttcgcttca agtaaataat ttcaaaatgg agggcagcag tcaattcctt
     1381 catgttaacg gcattgcttc aaaaagtgaa aaagacagct tgcaatttgc catgaataat
     1441 ttggatttgt attttatcat gaacgatttg gtaaaactca aggatccgtc gctgggagga
     1501 tattttacgg gcaatgcaac catgtacagc gcatttcagc aacctttttt cgaagctaaa
     1561 ttattggtaa aggatttttc catgaaccaa tcggttttgg gagatgtgaa tttgtattcc
     1621 tattgggaca aagaaaaaaa agaagtggtt tcaaatgctc atgtcttaaa caacaaggat
     1681 acggtagcct acattgacgg cgtttatgtg ccgggaaaag attccattga tttcgtgtat
     1741 gacatgcatc acctcaatat gggaatgttg aaacgatatt ttggtgatgt ggttcaaaat
     1801 gttagaggat ttgcaacggg caaacttcgg atgtttggac cgacaaaaaa aatgcgcttt
     1861 gatggagatt tgtatgtggc cgaaggacaa ttaaaaatgg cactaacgca aacaacctat
     1921 ttttttaacg ataccattcg tatgaggcct acttctgtcg agttcaataa tataaagatg
     1981 tatgatgaag aaggaaatga aggcttactt gatggaataa tgaaacatac aggaatgttt
     2041 ggcgacctga agtatgacgt aatgattcgc tccaataaca ttttggcatt gaatacccat
     2101 gcgaacgcca atgaaatatt ctacggaaaa gcttatgcag gaggtacggt tcatgttttt
     2161 ggtgatgtga atgttaccaa tgtttgggtg gacatggttt cacggccagg ttccaaatgc
     2221 tatgtaacta tgcccggctc ttcaactgct gccgacaaca gttttgttct ctttgtcaac
     2281 aaaaatgaca caataaagac ccagccggac gagcaaaaag ggaaaaaact gccagaaaat
     2341 acatacaata caaaggtaaa tcttcagata ggcattacgc ccgatgctga aattcaattt
     2401 attatcgatc cgaaagccgg cgatatgctt gttggaaaag gaacaggaaa tctgcgcttt
     2461 gaatttgata cctattccga cataaaactc tttggcactt acaccatcga aaaaggaaac
     2521 tatcttttta ccctccaaaa tgtgattcga aaagaattta aaattgatga aggaagtacg
     2581 ctgacttgga ccggcgatct ttataatgcg caggtaaaaa taaagggaat ttattctttg
     2641 atggcttctc tgcgggattt aatggatgaa tctcaattga gttcgttgcc tcgaaccact
     2701 gtgccggtaa attgtatatt gttgttgagt ggtaatttga tgaaacccaa tgtagaattt
     2761 gatattgatt tgccctcgag cgatgaaagt gtaaaacaga tggtaaaaag catcatcagc
     2821 accaacgaaa tgatggcgcg acaaattatt tatttgctca ttttcaacaa attttacaac
     2881 ccttatctgc agtgggctgc caacgctccc gtcggaattg gttccagcga agccgtatca
     2941 ttggttgtca gtactttaag tgcacaaatg aataattggc tggctcaaat gatcaacagc
     3001 aacattattt ccattggatt cgattaccgt caaacagatc agttgagcag tgatgttcag
     3061 gctcaatttc ttattcagcc aaataaccgg ctaattatca acggaaattt gggttatcga
     3121 aatgataatg taaatttgag caacaatacc aataaattta ttggcgacat tgacgttgaa
     3181 tggttactca cggaaggtgg taaattcaga ttaaaagcct acaaccacac cattgaccgc
     3241 tatcaacttc ggtcggcaaa gaatacgcaa ggaataggat tgatatataa agaaaatttt
     3301 tcaaattttg acgaattgtt ccgttattat tggaacattc tcacttttaa aaagaaaaac
     3361 aatgaagctg atcaataatt tttttacggt gatttttttc tttttgtgtg caaaaatttc
     3421 ggcacaaggc atcgataagt tgtatgtcgg catgccggat gcccttcatc cgactttgac
     3481 gtcgcaacaa cggctcgaac ttatcgaata cgccaaagcc ggacaaaacg ataccaccct
     3541 caatcgctat aaaaaaccgg tttttctgtt aaaactcgat acgcttcatc aacacttaat
     3601 ggtgaaaaat tcgggttcaa catcttttga aatgaaggtt ttggatgcca actcaaacga
     3661 accgatgatt gccattatcc gcacagtttg tgcaccaata tgtcattctt tgattgaatt
     3721 ctatgatttt aaatggaatc gccttcctgt aactttccat tatccccagg ccacagattg
     3781 gctcgatttg gaaaaagccc aaaaaactga agaaggggtt gaaacgtttc aaaaaatctt
     3841 gaaatatact tttgtttcgc tcagttttca ggaggaagga caaattgtaa tggctttcaa
     3901 caacagcatc gattttttaa gcgacgaaga taaaaaactc gtttttccct gcatgaatac
     3961 ggcacctttg ctgtttcaat ggagcgattt aaaatggata agggaaaaat aaaaaaggcc
     4021 tcaaaaaatt tttctgaagc ctttttacac aacattatta tgaaaaaaat tacttttgag
     4081 tttttttctt ctccaaactt ttagcgtagc gattcatgaa tttatcaaca cgaccggcac
     4141 tgtcaactaa tttggcttta cccgtaaaat aagggtgcga agaactggaa atttccaatt
     4201 taaccaatgg ataagtaacg ccatcaattt ctatggtttc cttagtatta atggtcgacc
     4261 gagtgataaa tgtgtcgcca ttcgataagt ctttaaatac taccggacga taattggtag
     4321 gatgaattcc gcttttcata tctgtcttaa atgtttgtat tttcttttga gtttgcaaag
     4381 atacgatttt tttctgaatc aacaaagagt tttctctttt ttctttttcc ccttcgatta
     4441 aaaatttatt cttttgattt ttaaaaagtt tttgccaact cctcggcctc ttttatggcc
     4501 ttattgacta ttgcatcaac atccatcccg atcacatcaa ggttagaagc tctgatggtt
     4561 gtataatcca atacaccaaa aagtcccata atggttttaa gatatttatc gcacaaatcc
     4621 cattctgcag ccattccttc ggaataatcg ccgccgctgg taataacatt aagcgctttc
     4681 ttgccttgac acaaaccaat tgggccattc tcgccatatt taaaagtgat attcggaata
     4741 cttacataat caatataagc ttttaatata gccgggaccc ccaaattcca cataggttca
     4801 gcaaaaatgt acttgtcggc acttttaaat tgcagagcgt attttttcat tgcattttct
     4861 gtttcttcca cttcattgct catgtgaatc atgacatcct caaccgttaa aaaatgaatg
     4921 tcttccttgt acaaatccag cgtaataatt tcgtcctgag gatggtgttt tttgtatgct
     4981 tccacaaatt tatcggcaat catgaatgtt ctggattcgc cctcaggttt agcattcgct
     5041 ttgatataaa gaactttact cataataaat tttcctccta tttttttgtt gttaacaaat
     5101 tcttatgcca ttgtcgtatt ttctctgatt tgactgcttt tagaaaaatt aaaaaaaaga
     5161 ttcaattcag ctgtttctat ttacagcggg gatgatttaa gacaaaaata atagaattct
     5221 ttcgtttgtc aaaaaaaatt cggaataagg gaaaaaaatt gtttctaaaa atctcaaccc
     5281 tatgtattca acattttaat aaaaacattt cttttgaaaa tcaaaaatat tcattacctt
     5341 tgtaaccgct ttaacaaata aaatttataa acataaaaag ctattaaaca gtatgttgaa
     5401 tcattatgaa accgttttca ttttaactcc cgttttatct gacctgcaga tgaaggaagc
     5461 ggtagaaaaa tttaaaacca ttttgcagga aaatggtgca acaattacca atgaagaaaa
     5521 ttggggctta cgcaagctgg cctatccaat cgaaaaaaaa tcctcaggtt tttattgttt
     5581 gctgcagttt gacgccgaac cgtccgtagt ggcagttttg gaaaccaatt atcgccgtga
     5641 tgaacgggtg attcgttatt taacgactcg tttggacaaa tatgcctacg agtatgcaga
     5701 aaaaagaaaa aatgtaaaat ctaaagaagg aaaggaataa actatggcaa ccaacaacaa
     5761 cgaagaaata agatatttaa ctcctcctac ggttgatatc aaaaagaaaa aatattgccg
     5821 attcaaaaag agtggtatta aatatatcga ttacaaggat ccggaatttt tgaaaaagtt
     5881 tgtaaacgag cagggaaaaa ttcttcctcg ccgcatcacc ggcacatctt taaagtatca
     5941 acgtagagtt gcgcaggcca taaaaagagc ccgtcattta gcgttgcttc cctatgtaac
     6001 agacatgttg aaataattta atcaggagga caaaaaatgg aagtaatttt aaaagaagat
     6061 gtaaataatc ttggttataa aggagatatc gtaaaagtga aagatggtta cgcgcgtaat
     6121 tatcttatcc ccacaaaaaa agccgttatt gcaaccgatt cggcaaagaa aatgctcgaa
     6181 gaagaaaaga aacaacgtgc tcataaacta gaacgcatta aaaacgaagc gcttgaattg
     6241 gcagaaaagg ctaaaagtat taatttggtt attgaagcca aagcgagttc aacaggaaaa
     6301 atttttggcg ccgtaggccc gattcaaatt gccgatgctt atgaaaaagc aggcttagtt
     6361 attgatcgca aagtaattgt tgtggacgaa ccggtgaaag aattaggttc ctataaagct
     6421 acgcttaaac ttcacagaga agtgagcgtc gaaatttcct ttgaagtaat cggcgtttaa
     6481 taatctgaaa gaataaactg tatttttaac ggaaggtgtc tctgttcaat gagatgcctt
     6541 tcgtttttta ttgattcata tcaacaaata ttttccatcc cataaaattt aaaattcacg
     6601 atttacattg gtatgtttac acaaaagttc gttaaaaaca ggattcatgc cgcaattgca
     6661 taaagtcaat aatttcttta gcaaattcat catttaaagt tctgttcgtt tgtacgtagg
     6721 acaagtatat aaatcttaca atcaaatgaa cattgtttat cattgtttta caggatgata
     6781 ttgaaaaggg aataactatt gcctttgctt tctatggtta atttcctttc aaacaaagac
     6841 aagttaaact ttagccaatt aaaatccttc gagaagttct tccagaattg ttgttctcag
     6901 tacttcccat atcttcgcaa ctgggaaaaa taagttcttc taatcacccc ttcatctatg
     6961 tttttcatgt gaaacttttt tatttaaacc attgattagc gttttattag gcgaacgaat
     7021 ttttagtgaa gtcttgatat aaaaaattga taattaataa ggtttattct tgttattaat
     7081 tatcgttact aaggttttac ttaaaaataa gcctcgtttt tggatagaat gactttatat
     7141 agaaacgatc aagcttagta acatgggaat acttcctttc agccttatta gcttaattat
     7201 aattgcttat taaatattct tatgccgaac ctcacgttaa aataatattt tttgagaaga
     7261 cttgtcgtaa aacggaacca tcggaagcgt atcctgagcc agacaaaaat caaaatcttt
     7321 ttccaaatga tgcgtaaaca accgttttgc atgttcactt tgctgcaaaa acgttttgat
     7381 atcgggctcg gcctgttgcc ataaaactaa tgcagcaact gccgaatcac ctgaagtttc
     7441 gaaatttccg ctgctcttca agctatggac gagcaagcct gcaaacaaag tatcttccaa
     7501 actgaaaaga ttattccagc cggcacaaac aacaagcaca tcttttccgg cattgcggca
     7561 aaaatcggcc actgcattaa tgttgcaaaa agccccgata attacttcgt tttcggctgc
     7621 tgacagttca atggcgcgcg tcccgttggt tgtagtaaaa acaatttctt tccccttcac
     7681 tttttcggaa taatagtcga acggagaatt accggcatct gcaaaatcgc acttttccac
     7741 attccgctcc gctccgacaa aataaccacg actttttaat tcttcggctt tttcgagcga
     7801 cgcaacggga ataatggcgg atgctccttc catcaaagct ttgcaaatgg tagtcgacgc
     7861 cctgaaaaca tccactatta ctgcaatttt tgaatttcct gacgaataaa aagggtataa
     7921 tgccggcgaa aaacaaacat ctattttcat ggggaataaa gtttttttac aagaaaccag
     7981 gtaaaaaaat tgaaaactac atttcaactt ctattatttt taatttttcc aaattaagtc
     8041 gcactttcag ccattcattc atctgggaca ataatgcttt tttattggga ggtgtttctt
     8101 cccattggat aaatatagtg ggaacagtat cttgtgttaa atgatccaaa ttgataaaaa
     8161 ttgaatttga aaaggctatt tgttttatat cgggaaattg caccttcact tcacgggcaa
     8221 gttgcaactc attaacccct gaagtttgca tcagttgcaa tttgttgttt aaagcagcta
     8281 ttattgaatc ttttttttga agttggatat ctttgtccat cagtaatttt tcgataaatg
     8341 cagtttgagc acctaagtcg aacgtccccc cggtttgctt gataataaga tttacattat
     8401 tcaaaccata cttgggaagt aattgttgta tgtatctgat ttgctcttcg gtcaaaggtt
     8461 tccctatgac agaaagtgta agagattttt cctttcttga atattttctc gaaacgttga
     8521 tgatttcctt ttcactaaac agatcgagtt gttggatatc ctgaatgtaa cgataggtat
     8581 gtgaattgaa tcttgtttcg ctaacaacat tccatgccag aaaaaaactg ggaataattg
     8641 ttaaaatgga aaatataata atagaacgtt ttacaacttt ttgtctttgt atgtcgacga
     8701 attgttttac tggaaaatga agtaaatgag ttaccaaaaa agtagctata gcaataaaaa
     8761 aagagttgat aaagagaaga taaaatgctc cgaagaaaaa acgtagctgt cccgtagcta
     8821 tcccatagcc ggctgtgcat aaagggggca taagagcagt agcgatggcc acacctgaaa
     8881 taacagttat tttcccttct tttcgagaag cagccacaat gccggcacat ccgccaaaaa
     8941 aagcaataaa cacatcaaat aaagtagggc gagttcgcgc taaaagttcc gattgggcat
     9001 cagtgatggg agtaataaga aaataaaacg tagaggcaac caatcctacc aatatcatta
     9061 taagcaagtt tcgcaatgat aacctcagga gttccgtatc ataaatgcct attgacaatc
     9121 ctactccatt gatgggtccc ataagcggag aaatgagcat ggcaccgata atcacagctg
     9181 tagaattaac attgagtcct acggaacaga tgataacagc aaaaaataaa atccagacat
     9241 tagccccctt aaattctaca tcttttttta tggaagctat ggtagattca atatttgtgt
     9301 cttcgtgtaa attggtaagt gtttttaaat cagatattat tttttgaaaa acatctttcc
     9361 attgaaaact ttttttttct tctgaagttt tttccataaa ttgtcaacat tgtaaatatt
     9421 tgttgtagaa taaaaaagtg agcttatctg aaaaattagt atcccgatat gcttaattca
     9481 tctattaatc gctgtgcgcc gccaattttg tccattacat aaagcacata cctgatatcc
     9541 acactgatgg tacgttgcag ctccggttca aaaacaatat caccactcat ggcttcccag
     9601 ttgccgtcaa aagccaaacc aattaattct cctttcccat tgaaaaccgg actgcccgaa
     9661 ttcccgcccg taatatcgtt attgctaata aaatttacat gcatgtcttt cgtctgagga
     9721 tccagatatt ttccaaagtc tttttccaga atcgactttt tcaacttatc gggaacaata
     9781 aattccggat cggttggatt ttctttttcc aaaatccctt tggttgtggt gtaataatgg
     9841 taatccacag catcggcagg ctgataacct ccgattttcc cataagtcat tcgcatggta
     9901 aagttggcat cgggataaag ttttttccct tgttcttcgt acatttccct aagccctgaa
     9961 aaataaatac gttcagcttc tttcaccttt tcggatgttt gagcataaat acccgaactg
    10021 atttgttcat acgtatttct tacttcgttg ccaaaaatca atgccggatc gttggcaatt
    10081 ttaattttct ttttctgtaa atttttcttc aatttatcta ccgaactaaa ggcggaattt
    10141 tggaaaattg aagctgcgta aagcgccgta tttcccttga attttttttg aatctcctga
    10201 taaaaagcag gaagtgcatc cgcatctacc gagtttttat aaacatccaa catggcagca
    10261 aaaacggctt gatcaacttc cggacggtaa tccttgtaaa tttcttctaa ttccttgatg
    10321 agagaatctt ttggcaagtt ttgcgaaata agttgacttg ctttctgggc aattctgggc
    10381 aattccacac cgctcatcaa tgattcacgc aaatagttga gcgcacgagc ataaggatac
    10441 agcgtagaat atccatcttc gagcgttttc aatacatctt tatatttttt ctctctttcc
    10501 ggattcgatt ttgtccattc caaaaaagca gcttcctgtt ccctttttct ctccagaaca
    10561 tttaatttct gaatggcttt attcatgccg atgctgtttt tccaatagtt ggaactcgaa
    10621 gcatatttat tggcataagc aatattgatt gcttcatcct tccgcataaa cgaacgccaa
    10681 atagtctgct tgatgcctct gacatcaatg cgtgcctgat tgccggtttc cattctgaat
    10741 ttcacgccaa aagaagtcaa gtagcggctg gtagagccgg gattacccaa aatcatggta
    10801 aaatcgttgg tttgataacc tttgttggaa acggacgcaa aacgtttcgg agaataggga
    10861 acattgtctt ttgaatattc ggccggtttt ccgtcgggag tcgcataaac gcggaaaacc
    10921 gaaaaatcgc ccgtatgacg cggccacatc caattatcgg tatcacctcc gaaatttcca
    10981 atggaactgg gcggtgcaaa agcaagtcga acatctttaa attcttcgta aacaagcagg
    11041 taaaattcgt ttcctgcaaa gaaaggtttc acttctgcca gataatgcgt gtccttttcc
    11101 gctgctttac aaatactgtc ggaaatttca taaatttttt cgtttctcga tttttcggac
    11161 aaactatccg gcaaatggga aagaacttga ttggttacat cttcaatttt aacaagaaat
    11221 ttcacactaa gaccgggcgt ggggatttca tcttccaatc gaacggcgtt aaatccatct
    11281 tttaaataat tatgttcaac cgaactgtgc tgttgaatgg catcaaaacc gcagtgatga
    11341 ttggtaaaaa tcaagccctg aggtgatacc acaactcctg tacaaccatt tccaaatacg
    11401 ataacggcat ctttaaggct gatattttta tcgctgtaaa tatcttctgc gctcaacgag
    11461 caacccattc cgttcatttt ttgaatattg agctgctcaa tgtaaggcaa catccacatg
    11521 ccttcgtcgg ctttggcacc tgtaaaaaga aaaaagatcg acaaaaaaac gagtgttctt
    11581 ttctttttaa caaaatattt ttcaaaagcc accataactt gtattaattt ttgatctgat
    11641 ataacttcat taaaccgtaa atatgttttt taccgggaac tgaaccattt ttctgtgctt
    11701 cgattttaaa aaattatcag taaaaaattt ttgcttgata catttgttta aattgatttc
    11761 tcaaacaaaa tttttttaga aaaagaaacg accatcttct atagaaaatt tattcgacaa
    11821 tggttgtcca gccataaata tcgggttctt cgccatactg aatggcacga atttttttgt
    11881 ataatttttc gcaaataacg cccggtttac catccggcga aaaaacgtag gatttatttt
    11941 cgtccaaatc gtcgattcgt tgaatagggc tgataacggc tgccgtacca catgctccgg
    12001 cttcttcaaa agtgctgagc tcttcaatgg gtacgggacg ctgttccact ttcatgccca
    12061 tgtctctggc caatgtcatt aaacttttat tggtgatgga aggcagcacg gaagtcgatt
    12121 ttggcgttac ataagtattg tttttaattc cgaaaaagtt ggcagcgccg cattcgtcaa
    12181 tatatttttt ctctttggca tccagataga aaactgccga ataacctaaa gaatgagctt
    12241 tttcgtcggc agccagccct gctgcataat ttcctcccac tttataaatt cctgttccca
    12301 gcggagctgc gcgatcgaac tctcttaaaa tgacgtatgg agttgtctga aagcctcctt
    12361 tgaaataggg tcctacaggg gttacaaata atataaaaag atattcgcgt gaaggtttga
    12421 cgccaacttg agcacttgtt ccaatcataa gcggacggat atacaacgaa gctcctgact
    12481 cataaggggg aacaaatcgt tcattcagtt tgacaacttt aagaacggct tcctgaaatc
    12541 tatcaacagg gaaaggttgc ataacaattc ctttgcaaga tgattgcatc cttttggcat
    12601 tttcatccat gcgaaaaacc ctgatttttc cgtcaatgcc tctaaaagct ttcaaccctt
    12661 caaaaacttc ctgaccgtaa tgcaggcaag tagctgccat gtgcaatgtc aggtattcat
    12721 cttcgtgaac ttcaaattct ccccattcac cattttgata actgcaacga atgttgtagt
    12781 cggttttcac ataaccaaat ccaatattgt tccaatccag ttccatattt atatgtttta
    12841 attttttgaa aaatccacaa aatcagtttc ttctttctca ttaaaaattt ttattctgct
    12901 tttcccaaaa gccataacag actttcattt cccatgttga atagcaagtc aatgatactc
    12961 agtcccgaaa taaatccgaa tttattttcg aacacctgat agtatgaagg aatttttaga
    13021 aagggctgtt tcttgggatg gaattgcaaa cgcaaatcca gtatattttc ttcaatttta
    13081 tcaataaact tgtccgtaaa ttccaacttg ggataaattt ccagcaattc gaaaattttg
    13141 ttctgaattt ccatgttgaa gtcccacaaa aataaccaat ttttttcata aaatgggcga
    13201 aaatcatcca aataatattc aaagaacgga ctggaaaggt aagcagaaga aattgctttc
    13261 caatgttgac gttgccaatc attgtgtccc gaaatccgaa tatcgcgaac agtagctttg
    13321 ttatttgcgg ttttttcaac cggaataatc agatccaaca ctccatttgc tccggcaatt
    13381 ctgcatcggt ttcgataggt ttgtttctga taatattcat gttgttcgat cacagccgat
    13441 cgggcatttg tcaaagtcat aaaataatct atcggagaca gataagacat agaaagcatc
    13501 actttctcct ctcgaattgc agagataaag tcgtgtaatt ccgaagagaa aatttctcga
    13561 atttttgctg acgataatat attttccgtt ttatcttcga gcatccttga aaaatctgtt
    13621 ccacctgatt tttccaccaa acaacgattt gtccttatcc aatgacaacc aaactactac
    13681 cggccgtccc acgatgtggt cttcaggaac aaatccccaa tagcgcgaat cagccgaatt
    13741 atgccgattg tcgcccatca tccagtaata atccattttg aaacgatagg ttttctctgg
    13801 tcgatcattg atataaaaaa ttccgttttt atattccagc ttgttgtttt cataggtgcg
    13861 aatcacccgt tcataaatgg gataattgta ggcattcaat tccagtactg ctcctttttt
    13921 gggaatgtaa atgggtccgt aattgtcgcg ggtccagttt ttacctccgc ccaatgggaa
    13981 aacatcgcca ccatacattt ccggttcaat cacaatttta acaacacccg taactcgttg
    14041 cagtttttgc agcatttcac ccgtcagcgg aaaatgataa atagggaaac ttggatccaa
    14101 acccaaagcc attatttcct gtgggttttg agaagtgttt aaaatcaaac ggtcatcctt
    14161 gcttattccc aatttggtga gtaattcatc actcaaacgt gttccatcgg tttgtaccaa
    14221 ataattgaac tgaacgcccg gaaatggttt ttgcttttct ccgttgatat aaacctgatt
    14281 gctttttatt tgcagccagt cgcctggcaa gccaatgcag cgtttaacgt aattttctct
    14341 gcgatcgaca ggtcgataca cgatttctcc aaaaatgtct tttctgctgc gaaccacatc
    14401 gcgtccgtag tagtagcaaa gttggtaata atcagggttt tgctgtttta gacaaaccgt
    14461 gtcgcccgtt ggaaaattga aaactacaat atcgttgtgt gcaatggtat cgaatccttt
    14521 caggcgttta tattcccatt ggggtttttc cagataggat tttgtgttga taatcggaaa
    14581 agtattctgc accaacggaa aagaaagcgg tgtattggga attcgcggcc cataactggc
    14641 tttgcttaca aatagaaaat ccccaaccag caaagttttt tcgagcgatg aggtaggaat
    14701 ctgataattc tgaaacaggt aaatattaat gaaatatact gctaccaaag caaatacaat
    14761 ggcatcaatc caactgtaaa tagaacgaag agttttgttc tttgtttttt tccaccaagt
    14821 ccagggaata aatttggtga taaacatgtc gaaaatgaca ggaagcagca acaaccacca
    14881 aaaattacct acccaagcaa tgaataaaat gtaaataaga ctccaaatgg cagcttttat
    14941 ccattgtcga cgaggttgat ggaaacggga tgtgtgaggt tcggaagtca taaatcaaat
    15001 tgttaagttt tttgaatttt tattgttttg ttcagttctg tgagaaatta ttgaactttt
    15061 tagcattcga ttttcctaaa tggagaaaat attaaaattt tagcaggtcc tgcatggtta
    15121 accatccttt tttcccgacg gcaaattctg cagccattac agctcccaga gcaaaaccat
    15181 cgcgggtttt ggcttcatgt ttaaattcta tggcatcaaa tgaagcatcg tagcgaataa
    15241 tgtggatacc cgggacttca tcttcacgaa ttgattttat ggccaattca tggggctgtt
    15301 ttgcttcttc ttttacccaa caggtctttc tttccaagtt ttccagcata tcttcggcca
    15361 atgtgatggc tgttccgctt ggagcatcca gcttatgaat atgatggatt tccgttattt
    15421 ccacatcgta atccgaaaaa cgattcatca gggaagccag atatttgttt acggccataa
    15481 aaatattgac ccccaaacta aaatttgatg accaaaagaa tgtgtagcct ttttcttcca
    15541 cttccttttt taaggcaggt aattcgtttg tccagcctgt agtcccagat accaaaggaa
    15601 tgcccgcagc aaaggttttt ctgtagttgc tcagcgctac gccgggatga gtgaattcta
    15661 tggccacatc ggcactttta aatttatcgg attcaaaatc attcaaattg tcaatgtcga
    15721 tgatacaaac aatttcgtgc ccacgttcct ttgctattcg ttcaatggct tttcccattt
    15781 ttccgtaacc aatcaatgct aatttcataa aaaattgaaa tattttgttg aaaaaatgaa
    15841 gttttaaaat cttgttgttt ttttgatttt cttcaatatt gttgtgtgct cagcaatacc
    15901 aacgtacatg cttcctgtgc ttgaatgccg tttgcttcca gcagggcttc aaattcagga
    15961 ttctcggttc cgggattaaa taccactctt ttcggatgga gtgataagat ataatcgtaa
    16021 tattcaggct gattttttgg acctacgtac aaagttaccg tatcaatgtc gggatattgt
    16081 tttttttccg tatcgatgga tttaccgaaa atttcgccgg gtcgaagtcc gagcaattct
    16141 atttcgtggc cattttcgag caaacgttgc gctgctttgt aggcataacg ttcaggattg
    16201 gtgctggcac ctattactaa agtttttttc attggttttt cattttttgt atttgatgct
    16261 aacatttttc gtaatgttgt ttttagtcac tttacatttc ctgataaaaa tccaaacatt
    16321 caaaaatttc tttttttgta gcagtttgat tcaattcaat tttccccact tctgacaata
    16381 aagtgaagtt gatggtatcc gaaacattct tcttgtcatg tgtcatcagt tcataaagtt
    16441 gttcgtaatc atcgcaacaa atagagattt ttccataatt ttcttttgca agtgtaacga
    16501 gctttaacaa atcttctttc ggaaagttca gtttcacgtg tgaaagatat aattcgcata
    16561 caagtccgta cataacggca tagccatgca aaaccggttg atgttgactg tacgaaaaac
    16621 tctcgaaagc atgaccgaaa gtatgtccca aatttaatgc ttttcgtaga ttttgttctt
    16681 tcgggtcctt ttcgactatt tgttccttta tttttacgct ttcggctact ctgtgtttta
    16741 aaagttcata atcaattgta tttaaattaa atttcagaat atttttccag tgttcacgcg
    16801 aatcgagcag cgaatgtttt atcatttctg caaagcccga acgcaaattc ttgtcgtcca
    16861 gtgttctgaa aaaatccacg tttatcagta ccttgtcagc cggagcaaaa acgccaattt
    16921 cgtttttcaa ccccataaag ttgatacctg tttttccacc ggtggccgca tctacagctc
    16981 caagcaaggt ggtggcaaca ttgatgtagc tgatcccgcg cttgaaagta gcagcagcaa
    17041 aaccgcccaa atcggaaacc attcctccgc ctgcgttaat catcaacgaa cggcgtgtgg
    17101 cgccatgtga tgataagtaa ttccaaactt caatggcagt atggatgttt ttattttcgt
    17161 caccactggg aatggttata acttggcaat tttttagttt ttccgattga agcaggagcg
    17221 gaagacagaa cttttttgtg ttttcgtcca ccaacacaaa aattccgtcc ggcttttgat
    17281 attcaatgaa ttcattcaac tcggcaataa aattttcaca tattatggtt gaagaagagg
    17341 tcatgtgtat gaatttctga tttaaaatac aaagatactt ttttctttaa aaacaaaagc
    17401 cataaatcag agacaatgta tttgttttaa taacattatg gaaataaaca gagtaaatgt
    17461 taaaaattct gaacttatta tagacaaatg aataaatgtt catatatttg cacaactttt
    17521 gaaatatgga acagattaac aatattaaca tagaccaaat ggaagaggca gcctataagc
    17581 taagagccat ttcacatgct acacgtttgt gtatcatttc gctgctttcg gagaaagagg
    17641 aattgaatgt ttcgcaaata agtgaagaat tgaattgtga acagtcgctg ctttctcatc
    17701 atctcaccga tatgcgtgcc aaaggcattt taaattgtcg tcgggatggt aagaattgtt
    17761 attattcgtt gaaaaacaag gaaatagttc aaattttgga ctgtattcgt tcttgtcagt
    17821 gcaccaacta aatttcgatt aactatgttg aaaaaattcc tttcgcgtca ttggctcaaa
    17881 gtagtcggag tgcttttggg gagcatcgga ggatttgcgt atttttattt tgttggttgt
    17941 aaatcgggaa cttgtcccat cagttccaat ccgtacatta gcattgtata tggagccgtt
    18001 ttgggttact tgttttttga catgtttaaa gtgaaaagta aaaacaccaa tgcgaacgat
    18061 caggaggatt aaaaaaatat tttttcagtg gaatatcgtc agaatgattc aactgatact
    18121 tgggattgtt ttgggaattg cttttatctc cactttagaa attgtttatt tgataatcgg
    18181 cagttttttg cttttgattg ctttgttcca tctaagtccc tgtgggcagt cgtgctctgt
    18241 ttcaactggt aaaaacgtcg gagaacgggg aaaatgaatt taccactata atgagataaa
    18301 ttaaaatccg atgagaaact tgtaaattta cgctttgaaa actaattttt gacaaaaaaa
    18361 taaaaaccaa aaataagaag aatatgaaaa aattgaccgg aatattttta atgatcactt
    18421 ttattgcttt aagcgcttgt gcgggaagca atggagaaaa caaacagaat aattcacaaa
    18481 acaaaaacga agaaaaacta atgggaacaa ttcatttaac aaaagaagaa tttttgaaaa
    18541 aggtagtcaa ttacgaagcc agccctaacg aatggaaata tctgggcgac aaaccttgcg
    18601 tgattgattt ttatgccgac tggtgtggac cttgcaaggc agtggctcca atattggaag
    18661 aactggctaa agagtatgat ggacagattt atatttataa aattgatacg gataaggaac
    18721 aggagctggc tgcagcattt ggcattcgta gcattccaac tttacttttc tgtcctatga
    18781 atgagaatcc gcaaatggct caaggcgctt tgccaaaaga tgtgctgaaa aatgccattg
    18841 atgaggtatt gcttaagaaa gaaaaagtaa aacaataaga caacccgaaa ggaatggaag
    18901 aatttttttt ggaaaagaag agtaaattca gcttccacaa aaccctctct gaattgacaa
    18961 atgctattca ggaaggaggt tggagactgg tttatgtgca tgacctgcag gaaactatgg
    19021 caagaaacaa taaagaagtt ttacctgtaa aagtgttgga actgtgttct ccatatcatg
    19081 cctattccat tttgggaaaa gatgaatggc gcactttttc gagcatgatg ccgtgtagaa
    19141 tttcaattta cgaaaaatcg gatggaaata cttacgtgtc tcaaatgaat atttcaatgc
    19201 ttgcacctct aatggaaaaa gaaattcagg gaatcatgaa catttcttat gcagaagtgc
    19261 aaaattttgt ttcacgaata atcgaaaact gatttaaaga caacaagttt aaaacattta
    19321 tttggaacgg aggtaaaaat ttttttgctt ccgttttttt gatgtatata ttgatttttt
    19381 taaatggatg aaagagccaa ttatcttatt ctttgatggt taaaaatttt ctcataaaac
    19441 tgattgaaat cagaaaatcg attgttggct acaaaatgga ttgaagtttt atctgcactt
    19501 tttgtgcagt tctgccaaat aaatctgttc ggtctgtccc ggagtaggga tgagtattgc
    19561 tttgtccaag cagttgagtg cttgcaaatc catgatggtt gagtatcccg aacgagcaat
    19621 aatttttttt gcgcctgcta ataaggacgc catttcttga gtggaaagat gagaaacttt
    19681 ggttaagttg ccaacttgta cggaagtttg tttttcctgt ggcaatccct gaatcatcaa
    19741 cgatttgaca gtgtctttgc tgaatttttc tgtcagttgt ttctcgaaaa tggtacgttg
    19801 cggttccgga ccggagagaa ccatcaccgt ttcgtaagta tagtttggaa taatattttt
    19861 caattgttga aaacgagata gaatgccaat aaaatgtgca ttttcgggta aaggataacg
    19921 atgtgccaaa tctcccgaaa agttttcatc tccggccaaa tcgggaatcc agcattcgtc
    19981 gtaacgatga atgaaaaagc gatgaagtcg ccagacaatc ggttccagca agcgcaattc
    20041 ggacggcatt tttaccatta gctgatgcgt aatgtaaatg gaatacgtcc ttttgttcca
    20101 acatccaaaa cggttgtcgg aaataatgcg atcgaaatgt tcctgtttta aaagtcggtg
    20161 aagccaacaa tgatcttgaa aaatattgcc aaaaatacgc gggatgcttt taaacatagc
    20221 ccagatttga gaatttcccg tgctgtaacg aacattttga gtaggagctt tcaaaaatcg
    20281 aagttcgggg aactcaagtt gaagaaattc taatgggaaa ccatctgctg caatcaccac
    20341 atcattgcct tctgtaatca gttgacgaat aatcggcaca cagcgtgcag catgtcccaa
    20401 tccccagttt aaaggacaaa cgagtatttt catcttcggc tttggtaatt atcctgaaac
    20461 ggaaacgtca aatccgtaac tttttgcttt ttcggctata cgattcagtt cctccaacgg
    20521 aattttttcc aaagttttca aaggagtttc tctgtcgatg gtataaatca taagctgcgt
    20581 tgcattcatt tcctttaatg cttccagcca tgcttttatt tccttatccg aagtattgtc
    20641 aattttttct ccgttcactt cgccacgaat aaataaagtt tgcacaatca ttttaccttc
    20701 aaactttttc aaatgttcaa tcagccagcg aacagtgatg tattttctta ccggacggtc
    20761 gagcagttgt aaagtatgat cgaaagcact gtcgaatttc agaatgttgt catccacttt
    20821 ttttaaggca ttgaaaacgt ctggtttgtg aatttgtgtg gcgttcgaca acactccaac
    20881 tttggcattg ggacaatatt tatttctcag ttcaagcgta tcgtcaataa ttccggcaaa
    20941 atcgggatga agtgtcggct ctccgtttcc ggcaaaagtt ataatatcgg gtatttgtcc
    21001 gtcgcttgcc atttctttca acctcatttc cagcgcattg cgtacttcca ttcttgtagg
    21061 aagtttggcg tgctttccgg tagtattaaa tccgcattcg cagtacagac agtcgaagct
    21121 gcaaattttg gcatctacag gcaataaatt gattcccaga gaagtaccaa gacgcctgct
    21181 atgaacaggc ccaaaaacaa tctgatcaaa taaaatcatt cttctacgat attttcaaat
    21241 aacgttaaat tattttgcga aaaaattgct ttcctttcaa agcctaccca gcaatatttt
    21301 ttcttttttc ttttccataa aagtaggttt taccttcttc cttcacatcg aaagtaacaa
    21361 tttccagttc gggacggttt tgaaaaaaac acaatagccc aaaatctcct gcggcaaaga
    21421 ggctgtgtaa accaaatatc gaaataaaaa aataaaatgc cggttgatga taaaagataa
    21481 tcattccaag cagcgaaaga aaactgatga aaacggttgg tgccaaagca attattttaa
    21541 actgacggta acccaccaca gccttgtcgg cttgaacata aaacatgaat tttttgggct
    21601 ggcagccaaa aaacaaatga cgagccccca ttgcccaata ggccagcgca tgaatcaatt
    21661 catgaaaaac gatgagaatg gtaaaggtga aaacaatgcc tgccagcagc caaagcaaac
    21721 cgaaggcttg acgatggaca aaataaggca tgaaggcttt gaaagttccc agcatgaaag
    21781 ccagaattcc tgtcaattgg tacaaatttg cagcacgtgc ccattgttgg ttttcggtaa
    21841 tttcgttgac aatgaaattt tttatttcgt tatgtgaaag agatgcgagt tctttgtact
    21901 cggtgtcatg aatcaattct tcgtatgtca tttatgaagc tgtttataat ttaatgacaa
    21961 tgcgttctcc aagatttttt gcaagttgat ttttaatttc ttccaactga ctgatttgaa
    22021 tcatcagaat acgaaccaac tcatcgtcgt ggttttctgc cgctattttc atttcgagca
    22081 atttttgctt aatctgcgag agaacaatgc tgtttttgta ttccagcacg gcacgttcgg
    22141 caaattccct cactttatcg atctcgggtt cgtccattct cgtttttttg taaattttgc
    22201 tgagggtgta tttttctgaa atcagttctg ccgtcaattt gctgatttcc ggatgaatat
    22261 gatgcaggaa aaaattttga acgttaatat tttttttcaa gcattgttcc ttatattcct
    22321 gaagcatttg agcatagagc ggatgaacaa attcaagctg atcctgttcg aggtcttcaa
    22381 taataaaatc ttctacagta atcggtgaac ggttttcttc gtcagaatag tacaatgccg
    22441 atttaccata tttaatcaat acttggagta tatttctttc ttcctgctca aacctctggt
    22501 tttcggcatt cagcatttcc tgaggagaaa cgggttgtgg ttttggttcg gtagaagaag
    22561 ccgaaacatt gctttgtcca agtttttttt cgctttcttt tcttttaagc cgatttattt
    22621 catggtaaag aatttcttcg ttcacttgaa gcagattgct gcattccttc acatattcgg
    22681 ctctcacaat cggttcggga ataatggcaa tacttcgcac gatgtccaga atcaggttgg
    22741 aacgtttgat ggggtcattt gctgcgtcct ccatcaaaag tttggttttg aagcggataa
    22801 aatccaccga attgtcccga atgaaattca agagatccac tgcattgagt ttgtgggaaa
    22861 aagaatcggg atcttctcct tcgggcaaca aaacaacctt tatgttcaag ccttcttcca
    22921 gcagcaaatc gattcctctc aacgaagctt taatgcccgc agcatcgccg tcgtagagca
    22981 cggtaacatt gttggtaaac cggtgaataa gacgaatttg agccggcgta agcgacgtgc
    23041 ccgaagaagc tacgacattt tcaatgccgc tttgatgaag agaaattaca tcggtataac
    23101 cttccaccag caaacagcgg tcttccttta cgatggcctg tttggcaaaa tacattccgt
    23161 agagctcatt tcccttgtgg tatatttccg attcgggcga attgaggtat ttagccgttt
    23221 tgtcgtcttt ttttattacc cttccgccga aagcaataat tttgccagaa agggaatgaa
    23281 ctggaaatat cagtcgtccg cgaaaacggt cggtaaggcg attgttttcc gtttcgatgg
    23341 taagtccggt ttttacaaga tattttttat tgtaaccggc ttttaaagct ttttgcgtaa
    23401 aagcatcctg ctgatcgagg caataaccca actgaaattt acgaatcatg tcttctcgga
    23461 taccgcgttc ctgaaaataa ctcataccga tggttttccc gtcgatgtga tgataaagcg
    23521 tatcggaaaa atatttctgg gcaaattcgt ttaccagcag caaactttcc cgttcgttgt
    23581 gaagttcttt ctcttcatcg gtcagctcac gttcctgcac ttcaatgttg tattttctgg
    23641 caaggaattt caatgcatcg taatacgaca gttgctcatg ctccattatg aagtgaacag
    23701 cgtttccacc ttttccgcaa ccaaaacact tgaaaattcc tttagcaggt gaaacggtaa
    23761 acgatggcgt tttttcgttg tgaaacggac acaaaccaat catgtttacg cctctttttt
    23821 tgagcgtaac ataatcggaa actacatcct gaatttgtgc agcttccata attctgtcaa
    23881 tggtggcttg gtctatcatg ctaatataaa gaattttgta aaaaaatcag tctctttgaa
    23941 atcaaataca aatctactga ttttattcaa gaatttagcc gagaaaaatt taaaaatttt
    24001 agatctgcgg ttttttcctt aatgatggag ggttgtgcca attttttgta gtgagttttt
    24061 caatttttaa aaaaataatt tgattagctg acaaaaaatt atttaatttg cagtttgaag
    24121 tttcaacata aaaaatatca ttacaatgaa agattttttg ccttatcttg gttctataat
    24181 cattttgata ggtgttgcat tgcttgcata ttatcaattc ggaggacatc cttctaatct
    24241 tattttagga actgcaggta ttctcatgat tgttggtttt tttactcacc ttttcctgaa
    24301 taaaaaaatt gtcgagtaac ctactgcaac agaaaaatta ccatttacct ttggaatttc
    24361 agtcggtagt aatcttgatt gatggggcaa agtggtcttt attgtttgtt ggtagaaaaa
    24421 tcagcgaatg atatgctgaa taaatagaac tgtcaaactg attccgtttt caaaaaaaga
    24481 atccgaactt caaaaatggt ttggattttt ttataaatat ttctaaagga tagcttacgt
    24541 ttacactttt catcagaata taatttattt tttaatggtc tgatggagcc acatgtctga
    24601 atattatatt cattcgtgtt tgtgtatgca atacatggag gtttctcaat gagttttttt
    24661 gacagaagaa atgtttctat cccgattgtg ttttcaaatt atcaggatag ggtttatttt
    24721 attcgtttca agcggcaaaa aagaatcaca tctttaatat cattaaatct gttccctgag
    24781 ttttccaaaa cagtgaattg gtaatttgta ccataaactt ttcttatctt tttcaaactt
    24841 ttttctccag tcagaaaata tccttctttc ggtttttctt tttcaaagtt tttgaaccga
    24901 ttgcccaaat aaaagtttag tgcgtataga tttggatatt ccttcaaatt gttcatcaca
    24961 aaaacattgt tttttaaaga atatttattt tgaagctttt cggcaaaagg acggacagaa
    25021 tagccatttt tataaatagg ataggcaaat ccttccagaa aaacttgtaa gccaataaat
    25081 aatcctatgc aagtaaaaag aatgttctgc acaattcttg tccgtttcca tttcagacac
    25141 caaacaaaag ctgttgccag aaaaagccag aacagaacac ctgcaactcc gggatgttga
    25201 aatgcttttg aatatagtcc gatatcgtga ttcaaggttt tatcttttat gagtggggag
    25261 gttatggcac ttaaatttac aaatcgggag aaggccatta acagtaataa aaatcccact
    25321 aaaatgaaaa tgatgtttgc tgtccaattg ataaattgag gtttttgctt cattgcccat
    25381 tcgtaaagaa aaacaagcaa ataggctgca aacggatata caggcattat gtaaacactt
    25441 cgctttgaac tcggtataga gtaaaagcca acaaccacaa ttaaagttat ggtgctgaat
    25501 aaaaccagtt tgtctgcatt tttcaaattc ttgaatgatt ctttcaattt tcgaaattgc
    25561 aattttttaa aaagcttttt gtacgaaacg gcaaacaaac tgaaaattgc cacaagtgac
    25621 cagggtaaaa aacctgtgag aattgcagga atgtaatacc aaaaaggatt ttcgtggcca
    25681 agattgtatg caatatccaa tgtttcgctt ttttgaccca aaaatcgacc gaaattttcg
    25741 gcatagacaa cattcagaaa tttttctcct ccttgccgat atgccaaata ataccaacat
    25801 aaaaggataa aaacagccgg aagagccaca acaatatttt tccagataat tttccagaaa
    25861 gaatatttct gaagcagcaa aaggtaaaca ataaaaacca aacacggtaa aacaatgccg
    25921 accggccctt ttatcagcga agctccggcc agcagcaggg aaataaacca aggaaatttt
    25981 cgcaaccctt tttcttccca aatataaaaa caaattaatg atgcaatcat aaaaaatgaa
    26041 agtagcatgt caactcgtgc ttcaatgccc gaacgatgca tttcaaagct tgtaagcatt
    26101 aaaagtgtgg caagtaaagc tctttttttc gatgtgcgct tggaaagaaa cacgaaaaag
    26161 gaaacagtaa taccaatcaa tcccaatgct gaaggcaagc gggctgtggt ttccgatact
    26221 tttccggccg gaagtgaaaa taaggcgatc atccaatgca tcaagggagg tttgtaggct
    26281 gtttcgtctg cataatcggt tggtagaatc caattgcccg tattgaggat acattgcgct
    26341 acagcggctt ctcgtggttc gcctttggtg taaaaatcgc tcaccatagt ccacggaata
    26401 gtcaacagca agctgagaaa caaaacaaat aagtagggat atttttgata gatggttttg
    26461 aaaaaattgt tcgatttttt catagatgta tgcagaaaaa ttttttaaac atttggtttt
    26521 gaattgtgaa atactcgaaa gatatttctt caaaaagcaa ttttattgtt tttttatctg
    26581 tttttatttg aatctttctt attaataata aatacatcct cattgtcttt tttatagaaa
    26641 taatgttctc tggcaaattt ttcaagattt tcgttactgg atttgagctc gaaaagtttg
    26701 gtacgatccg attttatttt ttcatcgtaa tatttaattt ctttttccag ttctttgttt
    26761 ttttggtaaa ttttccagcg tgaaatcaga ttgtgtttgt cgaaaaaagt tacgtatccg
    26821 gcaaaaatca aaaaaacaag ggtgtacttg ttcagaaaaa ttcgacgaag tattttcaaa
    26881 aattctttgc cggttgcaga cataaaattg aagagaaatt atggtgttga ttgaagaaaa
    26941 ttatatttct tgctcttaaa cttatttaga tgacaaaaat agtgaatttt gtgaatagaa
    27001 tttgttagga gaaaattatt tgtgtggaat aaggaataat ttttttttga ttatatttcc
    27061 tttatagatc aaaaaagtag cagaaatcat tcgaactgtt aaatcagcat agaagtgcga
    27121 atttatttct tttcggtact tttttattaa ctttgcagtc cgtttgaaga aagttttata
    27181 tttaataagt ttattttcaa ttatatcgtt atttaaaaat ttaaaattat taaacagaaa
    27241 gcacatgaaa gcatttgttt ttccgggtca gggggctcaa tttgtaggaa tgggcaaaga
    27301 tttatacgaa cagtctactc tggcgaagga atattttgaa aaggctaacg aaatacttgg
    27361 atttcgtatt accgatttaa tgtttgaagg aactccggaa gacttacgac agactaaagt
    27421 tacacaacct gccgtttttc tgcactcggt aatttcggct tttgtactgg gagaagaatt
    27481 taatcccgat atggttgcag gacattcatt gggcgaattt tcagcattgg tggcagctcg
    27541 tgccctgact ttcgaagatg gattaacttt ggtgtcgaaa cgtgctgtgg ccatgcaaaa
    27601 agcctgcgaa ctcgaagaat ctacaatggc cgccattatt gggttggatg atgaaattgt
    27661 cgaagaagtt tgtgattcga ttaaggacat gattgtggtg cctgccaatt acaattgccc
    27721 gggacaaatc gtcatttccg gtacattgca aggtgtggac atggcttgcg aaatgcttaa
    27781 ggaaagaggt gcgagacgag cactcaaact tagtgtgggt ggagcttttc attctccgtt
    27841 gatggaaccg gccagattgg aattgagcga agctattagt caaactacct tttctattcc
    27901 tatttgccct gtttatcaaa atgtgaatgc ttacccacaa accgatcccg ttaatatcaa
    27961 acaaaactta attgctcaac tcacagctcc cgtaagatgg acacaaacca tcaaaaacat
    28021 gattactgat ggagctgatg aatttattga attgggaccc ggagatgtct tgaaaggatt
    28081 gatcaaaaag attaatccca atgttatcgt tcactaattt atagaaaagt aaattaaact
    28141 tatcgaaaaa gatgtatgct gtaaagggtt tacatctttt tttgtaagta aaaatccaat
    28201 ttacgtaaat caaatgttat ttaaagcaat ggcaagctgt gatagaggaa aaaattgctt
    28261 ttttgggaaa aaaattaatg aattaacaag gaatgaaacc cacatgtatt gcatacacaa
    28321 acacgaatga atataatatt cagacat
//
LOCUS       DAOSIB010000021        25467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_2164_length_25467_cov_13.198410, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000021 DAOSIB010000000
VERSION     DAOSIB010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 25467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25467
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2164_length_25467_cov_13.198410"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLD44_03355"
     CDS             <1..270
                     /locus_tag="PLD44_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98409.1"
                     /translation="CAPKQRNFSYCCKNKHNVIDFKTIIKTFWNKYFVAIPVVFLLFH
                     ANLHKKNDFYRLLTKNKGRIVQWKIIVPLEIMVNMYIRIKFLTVT"
     gene            complement(601..1599)
                     /locus_tag="PLD44_03360"
     CDS             complement(601..1599)
                     /locus_tag="PLD44_03360"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOF98410.1"
                     /translation="MSKINAVITGVGGYVPDYVLTNEELSTMMDTNDEWITTRVGIKE
                     RRILKDKELGTSFMASKAVEDLLSKSGTNPDEIDMVLCATTTPDYIFPACAAIVTQNV
                     GIKNAVAFDIQAACSGFIVGLTTATGLIESGRFKKILVIGAEKMSSITNYEDRTTAPL
                     FGDAAGAVLVEPTTENVGIIDSILFTDGVGISHLNLKGGGSVYPASHETVDKRMHYIY
                     QEGQFVFKHAVSDMSDATVKILERNHLTKDDIAWLIPHQANLRIIDAVAQRIGLEYEK
                     VIINISRYGNTSAATIPLCMWESEKKFKKGDNIILTAFGAGFTWGAVYLKWGYTKE"
     gene            complement(1710..1895)
                     /gene="rpmF"
                     /locus_tag="PLD44_03365"
     CDS             complement(1710..1895)
                     /gene="rpmF"
                     /locus_tag="PLD44_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOF98411.1"
                     /translation="MAHPKRKHSNQRTAKRRTHDKAKLPTLAICSNCGATYIYHTVCG
                     ECGYYRGKLAIEKLANA"
     gene            complement(1915..2484)
                     /locus_tag="PLD44_03370"
     CDS             complement(1915..2484)
                     /locus_tag="PLD44_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOF98412.1"
                     /translation="MSKFELYNIVLKDLTNEKRIVEFDLDDAYFQKIDSPEVQQGNVH
                     AKITVQKKTTVYKLDFELNGFITLPCSRCLEDMQQLIHYKESLEVKFGNKFSEENDIV
                     IVPEADGAINVAWFLYEFIVLNIPLKHVHAPGECNKAMITKLKKHISSTKEYEDETSL
                     ELEDTNEDDYFSNNQFIDLEFDNLQNIDN"
     gene            complement(2608..3762)
                     /locus_tag="PLD44_03375"
     CDS             complement(2608..3762)
                     /locus_tag="PLD44_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOF98413.1"
                     /translation="MFSFNKIYRLSQRLRLLFIGTAALIVTASLFFTNRLTRQLATEE
                     HKKMEIWAGAMRQVILAGENTDINFLSEIISGNTTIPVIMTDENDNLISSLNVNEPAS
                     KTNDYYRREIERLKKKNSHIEVRIDNTLQHIYYDDSLLLKQLRYFPYIQLGVIFIFLL
                     IAYLAFSGTKKAEQNQVWVGLSKETAHQLGTPISSLLAWLDLLRIRNDDKLLTEMEKD
                     VNRLKIIAERFSKIGSAPDLQLVKLNETITNAVQYISHRSSQKVIITCHFQTSDNDLI
                     RMNVPLFEWVIENLCKNAIDAMNGNGKIDIFVQKKSNEYIVDVKDTGKGIEKNKFKAV
                     FSTGYTTKPRGWGLGLSLAKRIIEEYHKGKIFVKQSEIDKGTTFRILMKA"
     gene            4170..5594
                     /locus_tag="PLD44_03380"
     CDS             4170..5594
                     /locus_tag="PLD44_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric [FeFe] hydrogenase"
                     /protein_id="HOF98414.1"
                     /translation="MAFLNNTLIVRRDLMTKLVSLFNEGVLLSEIDRVPLKMSPKKAQ
                     ARGRCCIHKERAVLKYKIMPILGYSIEEEEDELTPLSEYARKALLRTEKNKSIFTVVD
                     EACTACVKTNYVVTNLCKGCVARACYMNCPREAVFFKENGQAGIDHNKCINCGICKEA
                     CPYHSIVFVPVPCEESCPVKAIQKDENGIEHIDESKCIFCGKCINACPFGSIFEISQI
                     FDVLSAIQRNEKITAIVAPSVQSQFEHSFSDIATAIKNIGFSEIIEVALGAMETAFHE
                     GEELQQKLEQGQQFMTTSCCPSYVELTKKHLIEMKPFVSQTNSPMYYASKIAKNKYPD
                     SKVCFIGPCVGKRKEAADDQTIDYVLTFEELDAILKGMKIEIIPTENKDDEEIKAGRG
                     FAQSGGVIAAVMESYPNVGIKPLIVTNIDKKNIALLKAYAKGKAPGNFIEVMACEGGC
                     ISGPCTINTYKDAQKIFKKVVEEN"
     gene            5721..6806
                     /gene="prfA"
                     /locus_tag="PLD44_03385"
     CDS             5721..6806
                     /gene="prfA"
                     /locus_tag="PLD44_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOF98415.1"
                     /translation="MAEKTLLEKLEGLQNKFDEISTLITDPNVISDQKRFAKLNKEYH
                     DLREILNVKNEYELALSHLTEAKQMVETESDTEMRELARSEIEELEQKIPELENKLKF
                     LLIPADPEDRKNAIVEIRGGTGGDEAAIFAGDLFHMYAKFCESKGWKIEVTDYNEGTA
                     GGYKEIIFTVSGENVYGTLKYESGVHRVQRVPQTETQGRIHTSAATVAVFPEAEEFDV
                     DLKESDIRVDIYCSSGPGGQSVNTTYSAVRLIHIPTGITVQCQDEKSQHKNKAKAMIE
                     LRSRIYAIEHQKYLDEIGSRRKTMVSTGDRSAKIRTYNYPQGRVTDHRINYTIYNLPA
                     FMNGEIQTMIDQLMIAENAERLKDAEL"
     gene            6836..7672
                     /gene="pyrF"
                     /locus_tag="PLD44_03390"
     CDS             6836..7672
                     /gene="pyrF"
                     /locus_tag="PLD44_03390"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOF98416.1"
                     /translation="MNRKELFENIKRKKSFLCIGLDTDINKIPIYLKEKSSNPIFDFN
                     KVIVDATADLCVAYKPNLAFYESLGIEGLKALNETVAYIRNHHPDQFLIADAKRGDIG
                     NTSAMYAQAFFEKMNFDAITVSPYMGEDSIKPFLSYKNKWTIILALTSNKGAFDFQLI
                     KEENTRERLFEKVLKTSTNWGTEENIMYVVGATKTEYLKSVREIVPEHFLLIPGIGAQ
                     GGSLEETAKFGLTSQCGLLVNSSRQIIYASSDENFAEAARFEALQLQQAMENILLKSG
                     II"
     gene            complement(7754..8974)
                     /locus_tag="PLD44_03395"
     CDS             complement(7754..8974)
                     /locus_tag="PLD44_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOF98417.1"
                     /translation="MKKILLSVMLLAVAVTYASAQSTAETKAKADIAHWSAGIKAGVD
                     YYRVTPSSTVTDYLFFENSWARYANNAAWTAPQIYVEYTFNPLFGLGADFSYLTYGRN
                     TGAGHTLDFTLYGSTNLSNLLAPKRAGFWGKVNIYGNLGAGLGFYSYSGVAGVTDGDN
                     LKSPLAFAALNAEYRLSKVLSLGVEGQYRYYMRENLGGLSNNSLGSDALIGTIGLRYK
                     FGGATKSHVRDMSLEEYYPAPAPVVIEKTVQDGKVDANTENRLKALENDNAMIKNKLQ
                     QLESDLKALSQKSTGTVNATFQNIEFEFGSSQLTKQAYDLLDQIYAILNSNSTAWTKI
                     TVSGHTDNIGPKSVNQQLSIARAQAVKDYLVKKGIPASAITVAGYGEDKPIASNDTPA
                     GRQKNRRVEFEISK"
     gene            complement(9364..9993)
                     /gene="pdxH"
                     /locus_tag="PLD44_03400"
     CDS             complement(9364..9993)
                     /gene="pdxH"
                     /locus_tag="PLD44_03400"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOF98418.1"
                     /translation="MLKDLRKQYDRSTLNESNILKNPFQQFKLWLSEAINTEESEPNA
                     MVLSTVDAEWQPHGRMVLLKELTDEGFVFFTNYKGNKGLQILHNQRVSLLFFWPVLER
                     QVRITGNAEKIAEQASDEYFKMRPLFSQIGAWASPQSRKIPSRDFLDKQFEYYKHKFE
                     TEVPRPPFWGGFLVRPKTFEFWQGRPDRMHDRLFYTKNDQQWEISRLAP"
     gene            complement(10097..10978)
                     /gene="era"
                     /locus_tag="PLD44_03405"
     CDS             complement(10097..10978)
                     /gene="era"
                     /locus_tag="PLD44_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009228927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOF98419.1"
                     /translation="MHKSGFINLVGNPNVGKSTLMNALVGEKLSIITSKSQTTRHRIL
                     GIVNEENYQMIFSDTPGILKPKYALQESMLQFSRSALTDADILMYVTDVYDAEPKDMD
                     FVQKINSDNVPLLVLINKIDLINQEKLELLVEKWHGIFPRGEILPISALEKFNVDYLM
                     KRIKELLPESPPFFDKDQLTDKPVRFFISEIIREKVLLNYQKEVPYAVEVEIEEFHEE
                     ENLVRIGAVIYVERDSQKGIIIGKGGKSLKKVGVQARKDIEEFLGQKVFLSLYVKVEK
                     DWRNKEKKLKDFGYLHD"
     gene            11332..11787
                     /gene="rplM"
                     /locus_tag="PLD44_03410"
     CDS             11332..11787
                     /gene="rplM"
                     /locus_tag="PLD44_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOF98420.1"
                     /translation="MDTLSYKTISANKNTVQKEWVLIDASDEILGRMGTKVAKLLRGK
                     YKPNFTPHVDCGDNVIIINASKIKLTGNKWTDRIYLRHTGYPGGQRETTPAQMLAKDP
                     ERLIRKVIKGMLPKNRLGAKLLKNLYVFPNAEHNMQAQQPKQIDLNSLK"
     gene            11793..12179
                     /gene="rpsI"
                     /locus_tag="PLD44_03415"
     CDS             11793..12179
                     /gene="rpsI"
                     /locus_tag="PLD44_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOF98421.1"
                     /translation="MEIVNALGRRKAAVARVFVNEGTGKIIINKRELSVYFPSLLLQY
                     IVKQPLDKLNVTEKYDIKVNLKGGGFKGQAEALRLAIARALVKINPEDKPALKSEGFL
                     TRDPRIVERKKPGRPKARKRFQFSKR"
     gene            12348..13184
                     /gene="rpsB"
                     /locus_tag="PLD44_03420"
     CDS             12348..13184
                     /gene="rpsB"
                     /locus_tag="PLD44_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOF98422.1"
                     /translation="MSRTNFEELLEAGCHFGHLKRKWNPAMAPYIFMERNGIHIIDLH
                     KTAAKIDEAAQAMKQIAKSGRKILFVATKKQAKDLVAEKAKAVGMPYITERWPGGMLT
                     NFPTIRKAIKKMSTIDKMMTDGTFDNISKREKLQITREREKLEKNLGSITDLTRLPAA
                     IFVVDVMKEHIAVKEAEKLGIPIFGIVDTNSDPSNIDFVIPANDDATKSIEVILNALI
                     DAIQEGIEERKLEKVETEAAEAPVVKERKSRVNKRPRTQREDDEALKANVVNKFIKEE
                     EN"
     gene            13404..14222
                     /gene="tsf"
                     /locus_tag="PLD44_03425"
     CDS             13404..14222
                     /gene="tsf"
                     /locus_tag="PLD44_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOF98423.1"
                     /translation="MAVTLQEITKLRTMTGAGLMDCKNALIEANNDLEKAIEIIRKKG
                     QAIAAKRSGREASEGCVLAAAKDGFAAVLALKCETDFVAKNADFMALTQSILDVAMDK
                     KPATLDELKAFTINGRTIADLVVDESAITGEKVELDDYNFVKGGTTAAYIHQGNKLAT
                     IVAFKEENVDHQVARDIAMQIAAMNPIAIDRASVPAHIIETELEIGREKAREEGKPEN
                     IIDKIAEGRLNKFFQESVLLEQTSVKDSKITIGEYLKAHHATIIDFKRVSLNLD"
     gene            complement(14325..15668)
                     /locus_tag="PLD44_03430"
     CDS             complement(14325..15668)
                     /locus_tag="PLD44_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOF98424.1"
                     /translation="MSKIYDFTLSYSLFRSYVMFAFKKFFSRYIVMGKENIPETGPVI
                     FAPNHLNALMDALAVISVVPHKMPVVFLARSDYFNNKVIARFLHFCKILPAFRMREGL
                     ENVDKNNEIFEACVDVLNHNQAIGIMPEGNQGSKRKLRPLAKGIFRIAFMAQQKYGNQ
                     PGVKIVPVGLDWGNIDKYGEHIIINVGKPIEVCEYMNEYEENQPAAINQLRDRLRDGL
                     SEVTLDIATEKNYECFETATIAASAAMVEKMKLPNTEKYRFLARQKTARQLLEMEKNK
                     PETIDKLNVICKEYRENLRKLNVPSWLLDKNLKVSSLLLESLLLVITFPFFLIGLVLN
                     ALPFFSPDIIMKAFHINFPGGKGSLRFGLGMVTFPLFYILQSVLFYKKVKDSLWAVAV
                     FLPLQYYLGKCAFLWHKKFRKLKVKFRYHKLQVKNSLILQRTKLLHEEILQLVQS"
     gene            complement(15937..17775)
                     /locus_tag="PLD44_03435"
     CDS             complement(15937..17775)
                     /locus_tag="PLD44_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF98425.1"
                     /translation="MFKFFELLKRFIPPYKKYVFLNLLFNLLSTVFSLFSFAAIIPVL
                     QILFGLHQSNLSYQAWTWGDGWNQIISALKNNIFYYLEQMMTTAGPSMTLLYLGLFLI
                     VMTVFKTGTSYLSSYYIIPIRTGVLRDLRNQLYKKILSLPIGFFTEERKGDIMSRMTS
                     DVTEIETSIISSLEMIFKNPIIILIYLTVMFAMSWKLTFFVLILLPLSGWLIGMIGKS
                     LKQRSTLGQQQTGELLSQIEETLSGLRVIKAFNAEKKLEKRFAALNEKLRRTLNRINR
                     RYTLAHPLSELLGTVVIAILLWFGGVLILENHSSINAAEFIYYLVIFYSIINPAKDLS
                     KAGYSVQKGLASLERVDKILKAENTLPEPTESKKINSFNDRIEFKNVNFRYQSDWVLQ
                     DINLTIPKGKTVALVGQSGAGKSTLVDLLPRFYDVTEGEILIDGINIKDLNTKDIRSL
                     MGNVNQEAILFNDTFFNNIAFGVENVTLEQVMEAAKIANAHDFIMASENGYQTTIGDR
                     GSKLSGGQRQRISIARAILKNPPILILDEATSALDTESEKMVQEAIEKLMMNRTTLVI
                     AHRLSTIKRADEICVMSEGKIVERGKHDELIALNGYYKRLYDMQSF"
     gene            complement(17813..19156)
                     /gene="purB"
                     /locus_tag="PLD44_03440"
     CDS             complement(17813..19156)
                     /gene="purB"
                     /locus_tag="PLD44_03440"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOF98426.1"
                     /translation="MNLSPLTAISPVDGRYRSKTEKYAEYFSEFALIRFRVMVEVEYF
                     ISLCEIPLKQLQLVPKDCFDKLRAVYIHFQESDAEKIKEIEQTTNHDVKAVEYFLKQQ
                     FDLLGLSDYKEFIHFGLTSQDINNTAVPVSIKKALEDVYYPEMENLLKKMRELAEEWK
                     DIPMLARTHGQPASPTRLGKEIMVFVYRLDKQLSLLRAVPISGKFGGATGNFNAHSVA
                     YPLVEWKNFADKFLAEKLGIQREEWTTQISNYDHLAALLDSLKRIDTILIDFCRDVWT
                     YVSMEYFKQKIKPGEVGSSAMPHKVNPIDFENAEGNLGMANAVLDFLSAKLPISRLQR
                     DLTDSTVIRNIGLPFSYILIATQSLMKGLNKLVLNESAIRRDLENNWAVVAEAIQTIL
                     RREGYPAPYETLKALTRTNEVITEQTIHDFIDSLQVSEEVKNELRKISPFSYTGI"
     gene            19448..20119
                     /locus_tag="PLD44_03445"
     CDS             19448..20119
                     /locus_tag="PLD44_03445"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOF98427.1"
                     /translation="MKKSIRIHKEGWFILTGLLLLLFAVNLIIYMEFPGPAMAINTVL
                     SAIILCFFVFFFRNPKRIVEIDDPSFVIAPADGTVVVVEKTLEPEYFGDERIQVSIFM
                     SIFNVHANWYPVSGTVVKTEHHNGRHLAAYLPKSSRENERSTVVIETPGKTQILVRQI
                     AGALAQRIVTYAHPGKECHVNEHLGFIKFGSRVDLFLPLDSEIFVQVGEKTTGNETII
                     ARLNE"
     gene            20131..20457
                     /locus_tag="PLD44_03450"
     CDS             20131..20457
                     /locus_tag="PLD44_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOF98428.1"
                     /translation="MTLQEAQQIVDNWIKTYGVRYFGELTNMAILTEEVGELARVMAR
                     TYGEQSYKAEDEKYDLADEMADVLWVLLCLANQTGVDLTDAFSKNIEKKTLRDAQRHI
                     QNKKLY"
     gene            20481..21368
                     /gene="deoC"
                     /locus_tag="PLD44_03455"
     CDS             20481..21368
                     /gene="deoC"
                     /locus_tag="PLD44_03455"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOF98429.1"
                     /translation="MKDRYEELLELYQCESVDDEAIKIDIDNILAEHFAENASKDVYA
                     KCLNLIDLTSLNSTDTEGEIVSMVEKVNHFRGTFPHLSNVAAICVYPALIPAVKSHLT
                     EENVGIASVAAGFPSSQTFIEVKIAETSMVVNEGASEVDVVISVGKFLEGNYGEVYEE
                     LSEIKAACRNAHLKVILETGALKNSVNIKKAAILAMSAGADFVKTSTGKIQVSATLEA
                     TYIISHAIKEWYEKTGQKIGYKPAGGISTTEEAVKHYTLVKHILGEEWLNNRLFRFGA
                     SRLANHLLSSIEGKEIKYF"
     gene            complement(21373..22347)
                     /locus_tag="PLD44_03460"
     CDS             complement(21373..22347)
                     /locus_tag="PLD44_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOF98430.1"
                     /translation="MNLIEKIRKPIEKEIEQFEKSMTEELQTENPLLKNISNYLQLGK
                     GKQLRPILVILSAKLCGEVTESTIYGAISLELIHTASLIHDDVVDNTFQRRGQPSINA
                     QWNNKVAILVGDHILSKSLSSGIKADNIKILETIANVGMNLTDGELLQLDKVQHAQIS
                     EEDYLEIIQKKTALLFSSCALIGGLSVKADKNTLKHLYHFGEYAGICFQMKDDLLDYS
                     ENNEIGKPTRNDIKDGKITLPLIYALKNATNHQKHEIEQWIENSDFSDLHIEKIVQFV
                     QENGGLEYTLQKMEEYKNKAIEELNGFADGELKQSMILFAEFMSNRDY"
     gene            22600..25467
                     /gene="polA"
                     /locus_tag="PLD44_03465"
     CDS             22600..25467
                     /gene="polA"
                     /locus_tag="PLD44_03465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOF98431.1"
                     /translation="MKKLFLIDAHGLIYRSYYAFIRSPRINSKGLNTSAIFGFVNTLE
                     EVLKNEQPTHLAVVFDPEGKTFRHEAYEQYKAQREKTPEDIRKALPIIKELVAAYNIP
                     AIEVTGYEADDVIGTLAKQAEKKGFEVYLLTSDKDLGQLVSDHIFMFRPHHNGGFEVL
                     GPDQIKEKYQLENQQQVIDLLGLMGDSSDNIPGCPGIGEKTAVKLLQEFGSIDNLLQN
                     TDKLKGSLKTKIEENKDLINFSRFLATIKTDVPVALDEEALKVGPADSEKLRALFQEL
                     EFRTLLNNKFGNTFSSFPPDYPLLQTPSGNKKSSKAEQMNLFGETNRPQKTEPEKEKI
                     LSKPEENIEEISPVASNGFMALKNIPHRYQLIDDKVKRSYLVSQLFLQKSVCFDTETT
                     GLDVFSADLVGLSFCFREGEAYFVTLPDNREEATKILNEFKAFFLSEQIEKIGHNMKF
                     DLLMLSNYGIDVKGKLFDTMIAHYLLQPELRHGMDYLAEIYLHYHTIHYEDLVGAKGK
                     NQLDIRNVEISKLTEYAAEDADVTFRLKQILEKELAANELEKLFYEIEMPLMKVLVIM
                     EKTGVRIDSEALRQSSEILTENMLKLEKEIYEMAGGEFNINSPVQVGEVLFDRLHLDD
                     KAKKTKTGQYSTSEDVLEKLRHKHPIVDKILEYRGLKKLLSTYIDALPQLIHPKTGKI
                     HTSYNQTNTATGRLSSSNPNLQNIPIRDELGKEIRKAFIPEENSLFLSADYSQIELRV
                     MAHLSNDQNMIEAFRNGLDIHAATAAKIYKVPLEQVTSEMRRQAKTANFGIIYGISAF
                     GLAERLGISRTEAKTLIDEYFLAYPQIKTYIESAIKKAKENGYVETLLGRKRFLPDIN
                     SQNSVVRGFAERNAVNAPIQGSAADIIKIAMVNIQRKLEKENLQAKMILQVHDELNFT
                     VPVNELETVRKLVVYEMEHAFPLSVPLIVDCGVGNNWLEAH"
BASE COUNT         8234 a         4673 c         4795 g         7765 t
ORIGIN      
        1 tgcgcgccga aacaacgtaa tttttcatac tgttgtaaaa ataaacacaa tgttattgat
       61 tttaagacaa ttataaaaac attttggaat aaatattttg ttgcaattcc agttgttttt
      121 cttctttttc atgcaaactt acataaaaaa aatgattttt acaggctgct caccaaaaat
      181 aaaggaagga ttgttcaatg gaaaattatc gtaccgttgg aaataatggt taatatgtat
      241 atccgaataa aattcctaac agtaacataa gcttacattt ataagtctca tgaatgacgt
      301 tgaatacatt atttccatgc agttgtttct ttgcttacaa aggatatatg aaacccacat
      361 gtattgcata cacaaacacg aatgaatata atattcagac atgtgggttc catcagacct
      421 ttaaaaaata aattatattc tgatgaaaat tcattgactt tttttatcac tccgcattag
      481 aattacatca aaactatttt ttctccattt atgaattttg gttatgtttt ttgtagaaat
      541 gaagaaaaaa aagcaggcga aaagcctgcc agaatgatta tttatataga aacactaaat
      601 tcattccttt gtataacccc atttcagata tacggcgccc catgtaaagc ccgcaccaaa
      661 ggctgttaaa attatattgt cgcctttttt aaattttttc tccgattccc acatacataa
      721 aggaattgta gcagcagaag tgttcccgta tcgactgatg ttgatgatta ctttttcata
      781 ctccagacca atacgttgag ccacagcatc tataatgcgt aaattggctt ggtggggaat
      841 tagccatgca atatcgtctt ttgtcagatg atttctttca agaattttca ctgtagcatc
      901 cgacatatcg gaaaccgcat gtttgaaaac aaactgacct tcctgataga tgtagtgcat
      961 gcgcttgtca acagtttcgt gagaagcagg ataaactgag cctccaccct ttaaattaag
     1021 atgagagata ccaactccat cggtaaataa gatagaatca atgattccga cgttttcggt
     1081 tgtaggttca accaaaacag ctcctgcagc atctccaaaa agaggagcag tagtgcggtc
     1141 ttcataattg gttatggatg acattttttc ggctccaata accagtattt ttttaaatct
     1201 gcccgattct attaaacctg tggctgtggt caaacccacg ataaaccccg agcaggcagc
     1261 ttgaatatca aatgcgaccg catttttaat tccaacattt tgagttacta ttgctgcaca
     1321 tgccggaaat atgtaatcgg gtgtagtagt agcgcatagt accatatcaa tttcatctgg
     1381 attcgtacct gatttggaca acaaatcttc gacagctttg gaggccatga aggatgttcc
     1441 aagttcctta tccttcagaa tgcgacgttc cttgatgcca acacgagtgg tgatccattc
     1501 gtcattggta tccatcatgg tgcttagttc ttcattggta agaacataat cgggaacata
     1561 tcctcccaca ccggtgatga ctgcattaat ttttgacata ttgtaaatat attaataaaa
     1621 aatcaagccc aaagccgaat ttcttcctca gggcccaatt gttttataca ttacgatttt
     1681 aaaaattttt tcaaaattca atgctttatt taagcattag ccagtttttc gatagccagt
     1741 tttcctctat aataaccaca ttcgccacaa acggtgtggt aaatatacgt tgcgccacag
     1801 ttggaacaaa ttgccagcgt tgggagtttg gccttatcgt gggttcttct ttttgcggtt
     1861 ctctgattcg agtgttttct cttaggatgt gccattttat tttatttttt ttatttaatt
     1921 gtcaatattc tgtaagttgt caaattctaa atcaatgaac tgattgtttg aaaaataatc
     1981 gtcttcgttg gtatcttcaa gttccaaaga agtttcatcc tcgtattcct ttgtggatga
     2041 aatgtgcttt tttagtttgg ttatcattgc tttgttacat tctcccggtg catgtacatg
     2101 tttcaatgga atgttcagta cgataaattc gtaaagaaac caagccacat ttattgctcc
     2161 atcagcttca ggtacgataa ctatatcgtt ttcctccgaa aatttgttgc caaactttac
     2221 ttctaaggac tccttgtaat gaatgagttg ttgcatatct tccaagcaac gggagcaagg
     2281 caaagttata aaaccattca gttcgaaatc gagtttataa accgtagttt ttttttgtac
     2341 ggttattttt gcatgtacgt tgccttgttg tacttcaggg ctatcgattt tctgaaaata
     2401 agcatcgtcc aaatcaaatt ctacaatgcg tttttcattg gtaagatctt tcaatacaat
     2461 gttgtaaagc tcaaatttcg acatgacaca ataactttaa aaagcggtac aaaggtacaa
     2521 aattctatat tattttgaaa ttttttccgt ttattttatc aattttataa gcaaacactt
     2581 tatataaaca tggtttgttg ctttttttca agctttcatc aaaatccgaa aagtggttcc
     2641 tttgtcgatt tcggactgct tgacaaaaat ttttcccttg tggtattcct caataattct
     2701 tttggccagc gaaagtccga gcccccaacc ccttggtttg gttgtatatc cagtggagaa
     2761 aacggcttta aatttatttt tttctattcc ttttcctgtg tctttgacgt caacaatgta
     2821 ttcgttagat tttttttgga cgaaaatgtc aatttttcca tttccattca ttgcatcaat
     2881 ggcatttttg cagagatttt caattaccca ttcgaaaagc ggaacgttca tccgaatcag
     2941 gtcattatca gaagtttgaa aatggcatgt gattattact ttctgcgatg aacgatggct
     3001 tatgtattgt actgcatttg tgatcgtttc attcagtttt actagttgaa gatcaggagc
     3061 tgagccaatt tttgaaaatc gttctgcaat aattttcagt ctgttgacgt ctttttccat
     3121 ttctgtcagc aatttgtcat cattgcgtat tcggagtaga tccagccaag caagcagaga
     3181 agagattggg gttcccaatt ggtgagctgt ctctttcgaa agtcctaccc atacttgatt
     3241 ttgttcggcc ttttttgttc ccgaaaaagc cagataggct ataagcaaaa aaataaaaat
     3301 tacacctaac tgaatgtacg gaaaatatct taattgtttt agtaataaag aatcgtcata
     3361 ataaatatgt tgtaacgtgt tgtcaattct tacttcaata tgtgaatttt ttttctttag
     3421 ccgttcaatt tctctccgat aatagtcatt ggtttttgaa gcaggttcat ttacgttaag
     3481 agaagaaatt aagttatcat tttcatcggt cataataacg ggaatggttg tgtttcccga
     3541 aattatttcc gagagaaaat ttatatcggt attttctccg gctaagatca cctgtcgcat
     3601 ggcacctgcc caaatttcca tttttttgtg ctcttctgtt gcaagttgac gagtgagtct
     3661 gttggtgaaa aatagggatg ccgttactat caatgcagcg gttccaataa ataagagcct
     3721 taggcgctgg gataaccgat atattttatt aaaagaaaac atgaataata attatcttga
     3781 aaaaatatgg ttcaaattta catgatattt ttcgattctc ataaaaagtg gaaaaattga
     3841 ttgggattga aaatttggtt gatgtgaaaa actttcttta atgaagtaaa ttttgaattt
     3901 caaaattctt ttcaggggtg gatctttttt aaagatttcg ttggctttct ccactatgcc
     3961 tacaattgtt ctcaaatact ggttctttcc tcttcttgca taatttgagg atttcttcgt
     4021 aaaaaactat ccttatgttt tcagaaaatc gacatactta attaaggaaa aatttcttta
     4081 attcaaaagg aatccctaac tttgcaaaca aaaaagtaag gtttaagaat aaaactttca
     4141 agcaaaattc cactcaaata caaggaaata tggctttttt aaacaataca ttgatagtaa
     4201 gaagagattt aatgacaaaa ttggtaagct tgttcaatga aggtgtactt ttaagtgaaa
     4261 tcgatcgggt tcctcttaaa atgtcgccta agaaagcaca agcacgagga cgatgttgca
     4321 ttcacaagga aagagcggtg ttaaagtaca agataatgcc aattcttgga tacagcatag
     4381 aagaggaaga agatgaactc actccgcttt ctgaatatgc aaggaaagca cttctgagaa
     4441 cagagaaaaa taaatctata tttactgttg tggatgaagc ctgcacagca tgtgtaaaga
     4501 ccaattacgt agttaccaat ttgtgtaaag gttgcgtagc acgagcctgt tacatgaatt
     4561 gcccaagaga agccgttttt tttaaagaaa acggtcaggc tggaattgat cataataaat
     4621 gtataaattg cggaatttgt aaagaagcat gtccttatca ctccatagtt ttcgttcctg
     4681 ttccgtgcga agaatcatgt cccgtgaagg ccatacaaaa agatgaaaac gggattgaac
     4741 atattgatga atcaaaatgt attttttgcg gaaaatgcat caatgcctgc ccatttggct
     4801 caatttttga aatttctcaa atatttgatg ttttatcggc aatccaaaga aatgaaaaaa
     4861 ttacagctat tgtggctcct tccgttcagt ctcaatttga acattctttt tcggatatag
     4921 caaccgctat aaaaaatata ggattttctg aaatcataga agtagcgctg ggggcaatgg
     4981 aaacagcttt tcatgaagga gaagaattgc agcaaaaatt ggagcagggt cagcaattta
     5041 tgactacttc ctgctgtccc tcttacgttg aacttaccaa gaaacatctg attgaaatga
     5101 agccattcgt ttctcaaacc aattcaccca tgtattatgc ctctaaaata gcgaaaaata
     5161 aatatcccga ttcaaaagta tgtttcatcg gtccctgtgt tggaaaaaga aaagaggcag
     5221 ccgatgatca aacgattgat tacgtattga cttttgaaga attggatgct attttaaagg
     5281 gaatgaaaat agaaatcata ccaactgaaa ataaggatga tgaagaaata aaagctggta
     5341 gaggatttgc tcaatccggc ggagtaattg ctgcggttat ggaatcttac ccaaatgtag
     5401 gaataaagcc gttgatagtt actaatattg acaagaaaaa tattgctctg ctcaaagcat
     5461 acgccaaagg gaaagcaccg ggaaatttta tagaagtaat ggcttgcgaa ggtggctgca
     5521 tttctggtcc ttgcacgata aatacgtata aggatgcaca aaaaatattt aaaaaggttg
     5581 ttgaggaaaa ttgaaagaaa ttttaatttt gactaatttt gccgaaatca tttgaatttt
     5641 ttattaaaaa tttagctcaa attccattca aaagaattaa aattaacgtc aagatattac
     5701 ctgacgataa atcgtttaaa atggccgaaa aaactttact ggaaaaactg gaaggactac
     5761 aaaataaatt tgacgaaatt tcgacattaa tcaccgatcc gaatgttatt tccgatcaaa
     5821 aacgttttgc caagctcaac aaggaatatc acgatttacg cgaaatttta aacgtaaaaa
     5881 acgaatatga attggcatta tcgcacttaa ctgaagcaaa acaaatggtg gaaaccgaaa
     5941 gtgatacaga aatgcgtgaa ttggcaaggt cggaaatcga agaactggag caaaaaattc
     6001 cggaattgga aaacaagctg aaatttttat taattccggc ggatccggaa gacaggaaaa
     6061 atgccattgt cgaaataaga ggcggaactg gtggagatga agcagcaatt tttgcaggcg
     6121 acctttttca catgtatgcc aaattttgcg aaagcaaagg ttggaaaatt gaagttaccg
     6181 attataatga aggtacggca ggaggttaca aggaaataat ttttacagta tccggggaaa
     6241 atgtctatgg cacattgaaa tacgaatccg gtgtacatcg cgttcaaaga gttcctcaaa
     6301 cggaaacaca gggacgaatt cacacttcgg cggcgacggt ggccgtgttt ccggaagctg
     6361 aagagtttga tgtggattta aaagaatccg acattcgcgt agatatttat tgttcttcag
     6421 gacccggcgg acaatcggtc aataccactt attctgccgt tcggttgata catattccta
     6481 caggaatcac cgtgcaatgt caggatgaaa agtcgcaaca caaaaacaag gcaaaagcca
     6541 tgattgaact tcgttcaaga atttatgcca ttgaacatca aaaatatctt gacgaaatcg
     6601 gttcaagacg taaaacaatg gtatctaccg gcgatcgatc ggccaaaatt cgtacctata
     6661 attatccgca aggaagagtt accgatcatc gcatcaatta cactatttac aacctgcccg
     6721 catttatgaa tggggaaatt cagacaatga ttgatcagct tatgattgca gaaaatgctg
     6781 aaagattaaa agatgcagaa ttataacata tgggatattg ataaattcca acaaaatgaa
     6841 ccgaaaagaa ctttttgaaa atattaaaag gaaaaaatca tttctatgta tcggattgga
     6901 taccgatata aataaaattc ctatttattt aaaagaaaaa tcatcaaatc caatatttga
     6961 ttttaataaa gttatagtcg atgcgacggc agatttatgc gttgcgtaca aacccaatct
     7021 ggctttttac gaaagtctcg ggatagaagg tttgaaagct ctgaatgaaa cagttgctta
     7081 cattaggaat catcatcccg atcaattttt gattgccgat gcaaaacgtg gcgatatcgg
     7141 caacacttcg gccatgtatg cgcaagcttt ttttgaaaaa atgaatttcg acgccataac
     7201 cgtttctccg tatatgggtg aagattccat taaacctttt ctttcctata agaataaatg
     7261 gaccattatt cttgcgctaa cttccaataa aggagctttc gactttcaac tgataaagga
     7321 agaaaatacc agagaaagat tgtttgaaaa agttttgaaa acatctacaa attggggaac
     7381 ggaagaaaat ataatgtatg tcgttggcgc aacaaaaaca gaatatctaa aatcggtaag
     7441 agaaatcgtt cctgaacatt ttcttttaat tcccggtatt ggggcgcagg gaggaagtct
     7501 tgaagaaacg gcaaaatttg gattgaccag tcagtgtggt ttgttggtaa attcatcgcg
     7561 tcaaattatc tatgcctctt cggatgaaaa ctttgccgaa gcagctcgct ttgaagcatt
     7621 gcaacttcaa caggcaatgg aaaatatttt gttaaaaagt ggcattattt gagctgccac
     7681 aatttttttt caatttaaaa agggcgactt caaacttgga gtcgcccttt aattttaaat
     7741 caactattta tttttacttt gaaatttcaa attctacgcg acgatttttc tgtctacctg
     7801 caggagtatc atttgaagca ataggtttat cttcaccgta acctgcgaca gtaatggcag
     7861 aagcaggaat acccttttta acaagataat cctttacagc ttgagcacga gcaatcgaca
     7921 actgttgatt aaccgatttt ggtccaatat tgtcagtatg acctgaaaca gtaatttttg
     7981 tccaagcagt agaattactg ttcaaaatag cataaatctg atccagcaaa tcataagctt
     8041 gcttggttaa ttgggaagaa ccaaattcaa attcaatatt ctggaaagtt gcattaacag
     8101 taccggttga tttctgcgat aaagccttta aatcgctttc aagttgttgc aatttgttct
     8161 ttatcatggc attgtcattt tccaaagctt tcaaacgatt ttccgtattg gcgtcaactt
     8221 ttccatcttg aacagttttt tcgataacaa ccggtgccgg agccggataa tattcttcca
     8281 aagacatgtc gcgaacatga gattttgtag ctccgccaaa tttataacgc agtccaattg
     8341 taccgatcaa agcatcacta cctaaagaat tgttactcaa cccgcctaaa ttttctctca
     8401 tataataacg gtactgacct tccactccca acgacaatac tttgctgaga cgatattcgg
     8461 catttagagc ggcaaatgca agtggagatt tcaaattatc accatcagta actcctgcaa
     8521 cacctgaata agagtagaat cccaaccctg cgcccagatt tccataaata tttactttac
     8581 cccaaaagcc agcacgtttt ggagccagta aattacttaa gtttgtagaa ccatacaagg
     8641 taaaatcaag cgtatgaccc gcacccgtat ttctgccata agtcagataa ctgaaatcgg
     8701 cacccaatcc aaaaagtgga ttaaaagtgt attctacata aatttgtgga gcagtccatg
     8761 ctgcattgtt ggcataacgc gcccaagaat tttcaaaaaa aagataatct gtaacagtac
     8821 tggaaggcgt aacccgatag taatccactc cagccttaat accggccgac caatgagcaa
     8881 tatcggcttt cgctttagtt tctgccgtgc tttgagcaga tgcgtaagtt acagcaactg
     8941 ccaataacat aacgctcaat aagatttttt tcataattct acttttttag ttaattgatt
     9001 ttccgaatat tattttgatt aaaaaagata aataggattt attgaaaaca cacctcaata
     9061 aaacttaaaa atttatttcc tgcctctaat ttataattca agcatcggca ttcaccgcaa
     9121 aattaataaa attactttaa tgcaaataat ttttaataga aatatcggca aatatattta
     9181 ttttttttca aaatcaaaat aattttcgaa ctttttttct aaaaaattat ttataataat
     9241 caaaaaaatc acgcaacaat aaaaatttaa atcgaagaaa gtttttaaag caaaattttt
     9301 gattctaaag aaagattttt ttcagtaact ttttacaata attatgccat aaaatctgtg
     9361 ttcttaaggt gcaagtcttg aaatttccca ttgttgatcg ttttttgtgt agaacaatcg
     9421 atcgtgcatt ctgtcaggcc ttccttgcca aaattcaaaa gttttggggc gaaccagaaa
     9481 cccgccccaa aaaggtggtc gtggcacctc agtttcaaac ttatgcttat aatactcgaa
     9541 ttgtttgtca agaaaatctc ttgacggaat ttttcgactt tgaggtgatg cccatgcacc
     9601 aatttgactg aataaaggac gcattttaaa atattcgtcg gaagcttgct ctgcaatttt
     9661 ttcggcattg cctgttatgc gtacctgacg ctccaaaacc ggccagaaaa acaacagaga
     9721 aactctttga ttatgaagaa tttgcaatcc cttattccct ttatagtttg taaagaaaac
     9781 aaatccttcg tcagtcaatt ctttcaataa aaccattctg ccatgaggtt gccattctgc
     9841 atcaacagta gatagaacca ttgcattggg ttcagattcc tcagtattta tggcttcgct
     9901 cagccacaac ttaaactgtt gaaaaggatt ttttaaaata ttcgattcat tcagagtaga
     9961 tcggtcgtac tgtttgcgta aatctttaag cataaaagca atttattaca cctttatact
    10021 caaataacac ttttaagaaa tatttgtttt aaatcgaaag agaaaattta taacaatcct
    10081 caacaaaatt ataggattaa tcgtgcaaat aaccaaagtc tttaagcttt ttctccttat
    10141 tcctccaatc tttttcgact tttacataaa ggctaagaaa aactttttgt ccgagaaatt
    10201 cttctatatc ttttctggcc tgtacaccaa cctttttcaa cgattttcca cccttaccaa
    10261 taatgattcc tttttgggaa tctctttcaa cataaatcac agcacctatt ctgacaagat
    10321 tttcctcttc atgaaattct tctatctcca cttcaacggc ataaggcacc tccttctgat
    10381 aattcaacaa cactttttcg cgaatgattt cactaataaa aaaacgtaca ggtttgtcgg
    10441 ttaactggtc tttgtcaaaa aaaggaggag attcgggcaa tagttctttg attcgcttca
    10501 taaggtaatc cacgttgaac ttttcgagtg cagaaatggg cagtatttca cctcgaggaa
    10561 aaatgccatg ccatttttca accaacaatt ccaatttttc ctgattaata agatctatct
    10621 tgttgattaa aaccaataaa ggaacattat cagaattaat tttttgaaca aaatccatat
    10681 cttttggttc tgcatcatac acatcagtaa catacatcag aatatcggca tcggtgagag
    10741 cagaacgtga aaattgcaac atggattcct gcaaagcata ttttggttta agaatgcccg
    10801 gcgtgtccga aaaaatcatt tgataatttt cctcgttgac tattcccaaa attcgatgac
    10861 gggttgtttg cgattttgag gtgatgatag aaagtttctc cccaacaagg gcattcatca
    10921 gcgtagattt acctacgttg ggattaccga ctaaatttat aaaacccgat ttatgcataa
    10981 tttaatacca actttatgat gatttcgatt catccacaaa attaaaaaat tattgattaa
    11041 agacgattat cagtcaaatt tttctacgct ccgaaaaaca caaaagcaaa tatcgaaaaa
    11101 atttcccgag ttgaaataaa aacataaaaa tcattttaaa aaaaatttta cctctaacta
    11161 tttgtaaatc gaaagaaaaa gagtattttt gcagtccgtt tattatccaa aataaaaagt
    11221 tcattggtat aatttattgt ctttattttg tattgattag ccattccaaa aataagataa
    11281 aaggtaccta agtaaaaaga atttattaat ttatttaaac cattagtaaa agtggatact
    11341 ttaagttata aaaccatttc tgccaataaa aacaccgtgc agaaagaatg ggttttgata
    11401 gatgcaagtg atgagatatt gggtcgcatg ggaacaaaag tggccaagct tctgcgtgga
    11461 aaatataaac caaatttcac accacatgta gattgtggtg ataatgtgat tattatcaac
    11521 gccagcaaaa taaaattgac aggcaacaaa tggacagatc gtatttactt acgacatacc
    11581 ggttatcccg gagggcaaag agaaaccacc cctgcccaga tgttggcaaa agatcctgaa
    11641 agacttatcc gtaaggtaat aaaaggtatg ttgcccaaaa acagattggg agcaaaactt
    11701 ttaaaaaatc tttacgtatt ccccaatgca gaacacaata tgcaggctca acagccaaag
    11761 caaatcgatt taaactcact taaataatca gtatggaaat agtaaatgcc ttaggaagaa
    11821 gaaaagcagc tgttgctcgt gtttttgtta acgaaggaac aggaaaaatc ataatcaaca
    11881 aacgagaatt gtccgtttat ttcccatcgc ttcttttgca atacattgta aaacaacctt
    11941 tggacaagtt gaatgtgact gagaaatatg atatcaaggt taatttgaag ggaggcggat
    12001 tcaaaggaca agctgaagct ttacgtttag ccattgctcg tgctttagta aaaataaatc
    12061 ccgaagataa acctgctttg aaatcggaag gtttcttaac tcgcgacccg agaattgtag
    12121 aaagaaaaaa accgggtcgt cccaaagcac gcaaaagatt tcagttctca aaacgataat
    12181 atcaatctgt tgtttctctt tttattatca aatgatattt gtaacaaaaa gtcacacaaa
    12241 cagcgaattg aatttggttt agtatctaaa ttgtggagac tcttttaaaa ataaagacta
    12301 ctcaacaatt gaatttcaca gaaagtaaac aaattaaaaa aagtaaaatg tcaagaacaa
    12361 attttgaaga attattagaa gctggttgtc attttggcca tttaaaaaga aaatggaatc
    12421 ctgcaatggc tccatacatt ttcatggaac gaaatggtat tcacattatc gatttacaca
    12481 aaacagctgc aaaaattgat gaagctgctc aagcaatgaa gcaaatagca aaatcgggaa
    12541 gaaaaatact atttgtagct accaaaaagc aagcaaagga tttagtagcc gaaaaagcaa
    12601 aagctgtagg aatgccttat ataacggaac gctggccggg tggcatgctc accaactttc
    12661 ctaccatacg taaagctatc aagaaaatgt cgaccataga caaaatgatg actgatggta
    12721 catttgacaa tatttcgaaa cgagaaaaac tgcaaattac acgcgaaaga gaaaaattgg
    12781 aaaaaaatct tggttctatt accgatttaa cacgtttgcc tgcagccatt tttgtggttg
    12841 atgtaatgaa agaacatatt gcggttaagg aagccgaaaa acttggaatt cctatttttg
    12901 ggattgtgga tactaattct gaccccagta atatcgattt tgttattccg gccaatgatg
    12961 atgcaaccaa atccattgaa gttattttga atgctctaat tgatgctatt caggaaggaa
    13021 ttgaagagcg aaaactcgaa aaagttgaga ctgaggctgc agaagcacct gtagtgaaag
    13081 aaagaaagtc cagggtaaat aaacgaccca gaacacaacg tgaagacgat gaggctctta
    13141 aagccaacgt ggttaacaaa tttataaaag aagaagaaaa ttaaataatg agaacatttg
    13201 actgagaaaa attttaattt aataaattcg ttcaaaagta ttattattta cagtcttgat
    13261 ctataataac gagttacggt tataattcat ttgtagctgg tagctcgttt tagttacaac
    13321 aacatttata aacaattaaa acatcttttt tttcatgtat gcatgaaatt gaagatattt
    13381 ttcagaattt aaatcaaaaa attatggcag taacattgca agaaattaca aaacttcgta
    13441 ctatgactgg tgcaggtctt atggattgta agaatgcgct gatagaagca aacaacgatc
    13501 tggaaaaagc aatagaaatt atcagaaaaa aaggtcaggc aatcgcagcc aagcgcagcg
    13561 gaagagaagc atcagaaggt tgtgtgcttg ccgcagccaa agatggcttt gcagcagtac
    13621 ttgccctgaa atgcgaaacc gacttcgttg ccaaaaatgc tgactttatg gctttaactc
    13681 aatccattct ggatgtggca atggataaaa aaccggcaac acttgatgaa ttaaaagctt
    13741 ttactattaa cggtcgtacc attgccgact tggttgtgga cgaatcggct attaccggcg
    13801 aaaaagttga attggatgac tataattttg taaagggcgg tacaacagcc gcttatattc
    13861 atcaaggaaa taagctggca actattgttg ccttcaaaga agaaaatgta gatcatcagg
    13921 ttgcacgcga cattgccatg caaatagcag caatgaatcc aatagctatc gaccgtgctt
    13981 cggttccggc tcatatcatt gaaacagaac tcgaaatagg gcgcgaaaaa gctcgtgaag
    14041 aaggcaaacc tgaaaatatt atcgacaaaa ttgctgaagg cagactcaac aaattttttc
    14101 aggaatcggt tttattggaa caaacatccg ttaaagacag taaaattaca atcggagaat
    14161 atctgaaagc tcatcatgca actataatcg attttaagag agtcagtctg aatcttgatt
    14221 aaaaattttt tatatatgaa aagacaaaaa cgggagaaaa tgatttttct tccgtttttt
    14281 tcaaaatcaa aaaagttaaa atggtagaat ttcgctgctc tttttcagga ttgcaccaat
    14341 tgaagaattt cctcatgcag caatttggta cgttgcagta tcagcgaatt ttttacttgc
    14401 aatttatggt aacgaaattt aactttaagt tttctgaatt ttttatgcca tagaaaagca
    14461 cattttccca gataatattg caagggtaaa aatacagcca ctgcccaaag tgaatccttc
    14521 acctttttgt agaacaatac cgattgcaaa atgtaaaaca gaggaaaggt taccatgcct
    14581 aatccaaaac gcaaggagcc ttttccaccg ggaaaattga tatgaaaagc cttcatgata
    14641 atgtcgggag aaaaaaatgg caatgcattc aaaacgagac caatgagaaa aaatgggaaa
    14701 gtgattacga gcaataaact ttcgagtaaa agagaagaaa ctttcaaatt tttatccagc
    14761 aaccaggatg gtacgttcaa cttgcgcaag ttttccctgt attccttgca aattacattc
    14821 aatttgtcga tagtctccgg tttgttcttt tccatttcaa gcaattggcg agctgttttt
    14881 tggcgggcaa gaaaacgata tttttctgta ttgggaagtt tcatcttttc aaccattgct
    14941 gcacttgctg ctatggtggc cgtttcaaag cattcgtagt tcttttcggt ggcaatatcc
    15001 aaagtaactt cactcaaacc gtcgcgcagc ctgtcgcgaa gttgattaat ggcagcaggc
    15061 tggttttctt catattcatt catgtattcg caaacttcta tcggtttgcc aacatttatt
    15121 ataatgtgtt caccgtactt gtcgatattt ccccaatcca aaccaacggg aacgatttta
    15181 actccgggtt gattgccata tttttgttgt gccatgaagg caattcgaaa aattcctttt
    15241 gccagcggac gcaattttcg tttacttccc tgatttccct ctggcatgat gccaatggcc
    15301 tgattatgat tcagaacatc aacgcaagcc tcaaaaattt cattgttttt gtcgacgttt
    15361 tctaaacctt cgcgcatgcg gaatgccggt aaaattttac aaaaatgcag aaatctggca
    15421 atcactttat tattgaaata atcggaacgt gccaaaaaga ctaccggcat tttatgagga
    15481 actaccgaaa taacggccag cgcatccata agtgcgttta aatgattggg cgcaaaaatc
    15541 accggcccgg tttcaggaat attttctttt cccataacaa tgtatcggct gaaaaatttt
    15601 ttgaaggcaa acatcacata acttctgaat aagctataag ataatgtaaa atcataaatt
    15661 tttgacattg tatatctttt atattggttg ttaattaatt gcagctaaaa ttttctcgaa
    15721 aaatttttaa aattgccgtg tcaatcgaat tccgggaaag aggtttaaag taaatcatcc
    15781 atgtttcata taaaaaatta ttcgctgtaa acctcattca gaaaaaaatg aatttgagaa
    15841 caaaatctaa attttcctgc aaaaattgtt cctttcttca aaaagttttt ttgctcacct
    15901 caacaatcag tttttgaatt aatttctcaa ttctattcag aaagattgca tatcgtacaa
    15961 ccgtttgtaa tagccgttta aagcaatcaa ctcatcatgt tttccgcgtt ccacaatttt
    16021 tccttcggac atcacacaaa tttcgtcagc ccgtttaatg gtggacaagc gatgtgcaat
    16081 gaccagtgtt gttcggttca tcattagttt ttctatcgct tcctgcacca ttttttccga
    16141 ttcggtgtcg agagcagaag ttgcctcgtc caaaatcaga attggagggt tttttagaat
    16201 agcgcgtgca atgctgatgc gttggcgctg accccccgaa agcttgctgc cacggtcgcc
    16261 gatagttgtc tggtaaccat tttcggaagc cataatgaaa tcgtgagcgt tggctatttt
    16321 tgcagcttcc atcacttgtt ccaatgtgac attttcaacg ccaaaagcaa tgttgttgaa
    16381 aaaagtatcg ttaaacagga tggcttcctg atttacattt cccatcaatg agcggatgtc
    16441 ttttgtgttc agatctttga tattgatgcc gtcgatcaga atttctcctt ccgttacatc
    16501 gtaaaaacga ggcaacagat cgaccaaagt agattttcct gcaccggact ggccaaccaa
    16561 agcaacggtt tttcctttcg gtatggtcag attgatgtct tgcaacaccc aatcgctttg
    16621 atagcgaaag ttaacgtttt tgaattcgat gcgatcgtta aaagaattaa tttttttcga
    16681 ttcggtaggc tcgggaagtg tattttcggc tttcagaatt ttatcgacgc gttcaagcga
    16741 agccaaacct ttttgaacgc tgtaacctgc tttagacaaa tcctttgccg ggtttatgat
    16801 actgtaaaaa attaccaaat agtaaataaa ttcggcagca ttgatggaac tgtggttttc
    16861 caaaatcagt acacctccaa accaaagtaa aatggcaata actacggttc cgagcagctc
    16921 actgagtgga tgtgccaacg tgtagcgccg gttgattcga ttgagtgtac ggcgaagttt
    16981 ttcgtttaaa gctgcaaatc ttttttccag ttttttttcg gcattgaaag ctttaatcac
    17041 tctcagtccg cttaaagttt cttctatttg cgaaagcaat tcgccggttt gctgctgacc
    17101 caatgtagag cgctgtttca acgatttgcc gatcatcccg atcaaccaac cgctgagagg
    17161 caaaagtatc aatacaaaaa atgtcagttt ccagctcatg gcaaacatta cggtcaagta
    17221 tatcagaata atgatgggat ttttgaaaat catttccaaa gaactgatga tggaggtttc
    17281 aatttcggta acatcgctgg tcatgcgcga cattatgtcg cccttgcgtt cttcggtaaa
    17341 aaatccgatg ggaaggctga gaattttttt ataaagttga tttctcaaat cgcgcaacac
    17401 acctgtacgt atgggaataa tgtaatacga cgacagatag gaagtgccgg ttttgaaaac
    17461 tgtcatgaca atcagaaaaa ggcccagata aagcaaagtc atcgaaggac cggcagtagt
    17521 catcatttgt tcaagatagt aaaaaatatt gtttttcagc gctgaaataa tttgattcca
    17581 tccatcgccc caagtccatg cctgataact cagattcgac tgatgcaaac caaagagaat
    17641 ctgcaaaaca ggaataatgg cagcaaaaga gaaaagactg aaaactgtcg acaataaatt
    17701 gaacaacaga ttgaggaaaa catatttttt gtaaggagga atgaaacgct tgagcagttc
    17761 aaaaaattta aacattgaat ttaatatatc tttttgtaaa acaatacaat aatcagattc
    17821 ccgtataaga gaaaggggaa atttttctta attcgttttt cacttcttca ctgacttgca
    17881 acgaatcgat aaagtcatgg atagtttgct ctgtaatgac ttcgttggtg cgggtcaaag
    17941 cttttaaggt ttcgtaggga gcgggataac cttcacgtcg aagaatagtt tgaattgctt
    18001 cggctacaac tgcccagttg ttttcaaggt cgcgtcgaat ggccgattcg ttcaaaacca
    18061 gtttattcaa ccctttcatc aaactttgcg tggctatcag aatataagaa aaaggtaaac
    18121 caatatttct aatcacagtg ctgtcggtca aatctctctg caaacgagaa ataggaagtt
    18181 tggccgatag gaaatcgagt acggcattgg ccatgcccaa atttccttct gcattttcga
    18241 aatcgatagg attcactttg tgtggcatgg cagacgaacc cacttctcct ggttttattt
    18301 tctgcttgaa atattccatc gaaacataag tccatacatc ccgacaaaag tcgattaaaa
    18361 tggtatcgat tcgtttcagg ctatccaaca aagctgccag atgatcgtaa ttcgaaattt
    18421 gggtagtcca ttcttcacgt tgaatgccaa gtttttcggc aagaaattta tcggcaaaat
    18481 ttttccattc taccaaagga tacgctaccg aatgtgcatt gaaattgccg gtggcgcctc
    18541 caaattttcc ggaaatagga accgcacgaa gcagagaaag ctgtttatcg agccgataga
    18601 caaaaaccat tatttctttt cccaatcgcg tgggagaagc tggctgaccg tgagttctgg
    18661 ccagcatggg aatatctttc cattcttcgg ccaactcgcg catttttttc aatagatttt
    18721 ccatttcggg gtagtaaaca tcttccagag ctttctttat cgaaacggga actgccgtgt
    18781 tgtttatatc ctgagaagtc aatccgaaat gaataaattc cttgtaatcg ctcaaaccaa
    18841 gcaggtcaaa ttgctgtttc aaaaaatatt ccacagcctt gacatcatga ttggtagttt
    18901 gttcgatttc cttgattttt tcggcatccg attcctgaaa atgaatataa actgccctca
    18961 gcttatcgaa acaatctttc gggaccaatt gaagttgttt taaaggaatt tcgcaaagtg
    19021 aaataaaata ttctacttca accatcacac gaaaacgaat taaagcaaat tcagaaaaat
    19081 attcggcata tttttctgtt tttgaacgat aacgcccatc aacaggcgat atggcagtaa
    19141 gtggtgaaag attcataaaa attttaaaat aagaatgagc ggtttgtcgg taaataaaat
    19201 tagttttttg tgaaaaatca gtacaaattt aatcattttc ggcatattat tgaaagttta
    19261 tatcgtacct tgtagcaaca aagtatttta agtaaatttg caaactgagt tctcttcgtt
    19321 caaaatatcc cgacagggtt actttcaggc agttgtctaa aattgtattt ttaaacaaaa
    19381 agagaaattt tttttgttgt actattttca atcgaccaga gaaatctcat aaatcatttt
    19441 taaacagatg aaaaaaagta tcagaataca taaagaaggt tggtttattt tgacaggatt
    19501 gttgctattg ctgtttgctg tcaatttgat tatttacatg gaatttcctg gtccggcaat
    19561 ggccatcaat acagttcttt ctgcaattat tctctgtttt tttgtttttt tcttccgcaa
    19621 tccgaaaaga attgtggaaa ttgatgatcc gagttttgtc attgcaccgg ccgatggaac
    19681 agttgtagtg gttgagaaaa ctttggaacc cgaatatttt ggcgacgaac gtattcaggt
    19741 ttccattttc atgtcgatat ttaatgttca tgccaactgg tatcctgttt cgggaacggt
    19801 tgtaaaaacc gaacaccata acggacggca tttggccgct tatttgccca agtcgagcag
    19861 agaaaatgag cgttctactg ttgtgataga aactcccgga aaaacgcaaa ttctggtcag
    19921 acaaattgcc ggcgctttgg cacagcgcat tgtaacctat gctcatccgg gaaaagaatg
    19981 tcatgtaaat gaacatctgg gatttatcaa gtttggatct cgggtagatt tgtttctgcc
    20041 gttggacagt gaaatttttg ttcaggtggg agaaaaaact accggcaacg aaaccattat
    20101 tgcacgactt aacgaataat ttctgttgtc atgactctgc aggaagctca gcaaatagtt
    20161 gataattgga tcaagactta cggggtacgt tattttggtg aattgaccaa catggccatt
    20221 cttacggagg aagtgggaga attggcgcga gtgatggcac gaacttatgg cgaacagtcg
    20281 tacaaggcag aagatgaaaa gtatgattta gctgacgaaa tggctgatgt tctctgggtt
    20341 ttgctttgct tggccaatca aacaggcgtt gatttgacag atgctttcag caaaaatatc
    20401 gaaaagaaaa cccttcgcga tgcacaacgc cacatacaaa ataaaaagct gtattaatca
    20461 ttttttaaat tcacaacatt atgaaagaca gatatgaaga gttgttagaa ctttaccagt
    20521 gcgaatcagt tgacgacgaa gccataaaaa tagatataga caatattctt gcagaacatt
    20581 ttgccgaaaa tgcatcaaag gatgtgtatg ccaaatgttt gaatttgatt gatttaactt
    20641 cgctcaatag taccgatacc gaaggagaaa tcgtttcaat ggttgaaaaa gtcaatcatt
    20701 tcagagggac ttttcctcat ttgtccaatg tggcagccat ttgtgtttat ccggccctga
    20761 ttccggccgt aaagtcgcat ctgaccgaag aaaatgtcgg gattgcttcc gtagcagcag
    20821 gatttccttc ttctcaaact tttattgaag ttaaaattgc cgaaacttct atggtggtca
    20881 atgaaggagc atcggaagta gatgtggtta tttccgttgg gaaatttctg gaaggcaatt
    20941 acggcgaagt ttacgaagaa ctgtcggaaa tcaaggctgc ctgccgcaat gcacatttga
    21001 aagttattct tgaaaccggc gctttgaaaa atagtgtgaa tatcaagaaa gctgctattc
    21061 tggcgatgtc ggcgggagcc gatttcgtta aaacttctac cggcaaaatt caggtttccg
    21121 ccaccctcga agcaacgtat attatttccc atgccataaa ggaatggtat gaaaaaacag
    21181 gccaaaaaat tggatacaaa ccggcaggtg gaatttctac cacagaagaa gccgtgaaac
    21241 attatacgct tgtgaaacac attttgggcg aagaatggct caacaatcgg ttgttccgtt
    21301 ttggtgccag ccgattggcc aatcatctgc tcagttcgat agaaggaaaa gaaatcaaat
    21361 atttctaatg ctttaataat cgcggttcga cataaattcc gcaaagagaa tcatggactg
    21421 tttcagttca ccatcagcaa agccgttgag ttcttcaatg gctttgtttt tatattcttc
    21481 catcttttgc aaggtgtact ccaagccacc gttttcctga acaaattgta caattttttc
    21541 gatatgcaag tcggaaaaat cactgttttc aatccattgc tcaatttcgt gcttttggtg
    21601 gttagtggcg ttttttaatg cgtaaatgag tggtagtgta attttaccgt cttttatgtc
    21661 gtttcgggtt ggtttgccaa tctcattgtt ttccgaatag tcgagaaggt cgtctttcat
    21721 ctggaaacat attcctgcat attcaccaaa gtgatataaa tgcttcaaag tatttttgtc
    21781 ggcttttacc gaaaggcctc cgataagagc acacgaagaa aaaagcaggg cggttttttt
    21841 ttgtatgatt tctaaataat cctcctcgga gatttgagca tgttgaactt tgtccaattg
    21901 tagcagttct ccgtccgtga gattcattcc tacgttggca attgtttcca gaatttttat
    21961 attatcggct tttattccag aactcaatga ttttgaaagt atatgatcgc caacaagtat
    22021 agctactttg ttgttccact gagcgttgat ggatggttga cctcttcgct ggaaagtatt
    22081 gtccaccaca tcgtcgtgaa ttaagctggc tgtatgtatc aattctaatg aaattgctcc
    22141 gtaaatggta ctttcggtaa cttcgccgca caactttgcc gacaatatca ccaagatagg
    22201 ccgaagttgc ttgccttttc ccaactgaag ataattgctg atattcttta acagtggatt
    22261 ttcggtttgt aattcctctg tcatcgattt ttcaaactgt tcaatttctt tctctatggg
    22321 ctttctaatc ttttctatca ggttcataag aacagaagta aatgaaataa tctgcaaaag
    22381 taatcaaatt taaacgttcg ttggtcataa gctatccaat tatgcctttt ttttaattac
    22441 ttttacactc aaataaaaaa cgattttttt ctccatttgt ttggaaaatc tttcttcttt
    22501 gcatttttta attttcagtt agagaaattg caataaagaa tttttcattt tttctaattg
    22561 actacaactc acttttttgg gtgtaaaatt caaaaaccta tgaaaaaact ttttttaatc
    22621 gatgcacacg gacttatcta tcgttcctat tatgcattta ttcgcagccc gcgcatcaat
    22681 tctaaaggac tgaacacttc cgctattttt ggttttgtaa atacgctgga agaagtttta
    22741 aagaacgaac agcctacaca tttggccgtt gttttcgatc ccgaaggaaa aacttttcgg
    22801 cacgaagctt acgaacagta caaggcacag cgtgaaaaaa cgcccgaaga tatacggaaa
    22861 gcgttaccca tcataaaaga gctggttgct gcttataaca ttcctgccat agaagttacc
    22921 ggttacgaag cggatgatgt gattggtaca cttgccaagc aagccgaaaa gaaaggattt
    22981 gaagtttatt tgctgacttc cgacaaagat ttgggacaat tggtttccga ccatattttt
    23041 atgtttcgtc cccatcacaa cggtggcttt gaagtactgg gccctgatca gattaaggaa
    23101 aaataccaac tcgaaaatca acaacaggtg attgatttat tgggattgat gggcgacagt
    23161 tcggacaata ttccaggttg ccccggcata ggcgagaaaa cggcagtgaa attgcttcag
    23221 gaatttggca gcattgacaa tttgctacaa aacaccgaca aattgaaggg ctcgcttaaa
    23281 acgaaaattg aagaaaacaa agacttgatt aatttttccc gatttctggc taccattaaa
    23341 accgatgtgc cggtcgcttt ggatgaggag gcactcaaag ttggacctgc cgacagcgaa
    23401 aaattgcgtg ctctgtttca ggaattggaa tttcgtacct tattgaacaa caaatttggc
    23461 aatacgtttt cttcttttcc gcccgattat ccgttacttc aaaccccttc agggaataaa
    23521 aaatcttcca aagccgaaca aatgaatctt ttcggagaaa ccaataggcc tcaaaaaact
    23581 gaacccgaaa aggaaaaaat actgtcgaaa cctgaagaaa atattgaaga aatttcacca
    23641 gttgcttcca acggatttat ggccttgaaa aatatcccgc atcgctatca gttgatcgac
    23701 gataaagtga agcgttccta ccttgtttcg caactttttc tacaaaaatc ggtttgtttc
    23761 gataccgaaa ctaccggact tgacgttttt tcggccgatt tggttggcct ttcgttctgt
    23821 ttcagagaag gagaagccta ttttgtaacg ttacccgaca acagggaaga agcaacaaaa
    23881 attctgaacg aattcaaagc gttttttctg agtgaacaaa tcgaaaaaat tggtcacaac
    23941 atgaagtttg accttctgat gctctccaac tatggcattg atgtgaaggg aaaacttttc
    24001 gataccatga ttgcccatta tctgttgcaa cccgaattga ggcatggcat ggattatctg
    24061 gccgaaattt atctccacta ccataccatt cattacgaag atctggtagg agcaaaaggg
    24121 aaaaatcagt tggatattag aaacgtggaa atttccaaac ttacggaata tgctgcagaa
    24181 gatgccgatg ttacgtttcg cctgaaacag atattggaaa aggaattggc ggccaacgag
    24241 ctcgaaaaac ttttttatga aatcgaaatg ccgctcatga aagtgttggt aataatggaa
    24301 aaaacaggtg tacgcatcga cagtgaagct ttgcgtcaaa gttcggaaat tctaacggaa
    24361 aatatgttga aactcgaaaa ggaaatttat gaaatggcgg gcggtgagtt caatatcaat
    24421 tcgccggtac aggtgggaga agtattgttt gatcggttgc atttggatga caaagccaag
    24481 aaaacaaaga ccggacagta ttctacttcg gaagatgtgc tggaaaaact tcggcacaaa
    24541 catccgattg tggataaaat attggaatac agaggattga aaaaattgtt gagcacctac
    24601 attgatgcgc ttcctcaact gattcatccg aaaacgggga aaatacatac atcttacaac
    24661 caaaccaaca ccgccaccgg tcgattgagt tcgagcaatc ccaatttgca gaatattccc
    24721 attcgcgacg aattgggaaa agaaatcaga aaagccttta tcccggagga aaacagcctg
    24781 tttctcagtg ccgactattc gcagattgaa cttcgtgtga tggctcattt gagcaacgac
    24841 caaaatatga tagaggcttt cagaaacggt ttggatatac atgccgctac agcagccaaa
    24901 atttacaaag ttccgctgga acaagtaact tccgaaatgc gtcgtcaagc caaaacagcc
    24961 aatttcggaa ttatttacgg aatttcggct tttggtctgg cggaacggct cggtatttca
    25021 cgcacagaag ccaaaactct gattgatgag tattttctag cctatccgca gataaaaact
    25081 tacatcgaaa gtgccatcaa aaaagccaaa gaaaacggtt atgtggagac gctgctcgga
    25141 agaaaacgct ttttgcccga tatcaattca caaaacagcg tggtgagagg ttttgcggaa
    25201 cgaaatgcgg ttaatgcacc gattcagggc tcggcagccg atattataaa aattgcgatg
    25261 gtcaacattc agcgaaaatt ggaaaaagaa aatttgcagg caaaaatgat tttgcaggtg
    25321 cacgacgaat tgaattttac cgttcctgta aacgaattgg aaacggtgcg caaacttgtt
    25381 gtttacgaaa tggaacatgc ttttcctctc agtgttccgt taattgtaga ttgtggcgtt
    25441 ggcaacaact ggctcgaggc acattaa
//
LOCUS       DAOSIB010000022        25366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_2178_length_25366_cov_9.987950, whole genome shotgun sequence.
ACCESSION   DAOSIB010000022 DAOSIB010000000
VERSION     DAOSIB010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 25366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25366
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2178_length_25366_cov_9.987950"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..248)
                     /locus_tag="PLD44_03470"
     CDS             complement(75..248)
                     /locus_tag="PLD44_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98432.1"
                     /translation="MLKSTALARFKLHRGLTVKCSENLPERKARKRFIDLLPLSPAQC
                     ASCGEVGRNDFLQ"
     gene            complement(291..785)
                     /locus_tag="PLD44_03475"
     CDS             complement(291..785)
                     /locus_tag="PLD44_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98433.1"
                     /translation="MPNNEEIGKQASIDGFAHEHIVVGILMKRYQNVSLVDLPLSPYD
                     IIIVRKLANGKEDIIRVQVKTARTSVSFTGGSRGGVDREYKSDVKTYTQSPETSDVIV
                     GFRPNADNTFDLYFVPTVLIEYFGTKSKGLGTIEFFKNNYDILENCKDKTYCVKIAKE
                     NGII"
     gene            complement(787..2175)
                     /locus_tag="PLD44_03480"
     CDS             complement(787..2175)
                     /locus_tag="PLD44_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOF98434.1"
                     /translation="MNYYPISIKEIKEKVAKKKIFKQPVQYSQFGGLSNIKEITNEFG
                     ETLIRVDGDIPADLPLTDGQRFLFISYDQSKYSHGIHKYPAKFFPELPRWLINRYSKP
                     NDLILDPFAGSATTNIEALLLNRNSVAIDIDPFAKYLAKVKTTPIDKLELNKTVDRIL
                     KQLVTYNPTLVENKDIPEFPYRENWFAREILLELAFIKKIILNSTNDKNIKDLCLISM
                     SSIIRAVSNADDNCTRTVVRKKLKKEIYPSLALTKFAENLIVNSYRINDFTNRLKSNA
                     TVEIPDGNDARFLTYPDNHFDLAITSPPYVNAVDYPRTHQLEMYWLGLASGSLSKLKE
                     THIGTEVVRINEYKQLHRIGEEYADKKIEAIYKLDARRAYIAYKYLYDMTLNMKEVYR
                     VLKPGGRYIIVVGNNRIKGELFESWKYLMSLSKNIGYQVENYFGSEIIKHFIKVPREE
                     RINTDWIIVLKK"
     gene            complement(2423..4975)
                     /locus_tag="PLD44_03485"
     CDS             complement(2423..4975)
                     /locus_tag="PLD44_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOF98435.1"
                     /translation="MNKLVELILIYIPISVLLVLAGVFLGGEVWAKGKVVLGDILNFF
                     GWVGSSVRKLSVKSEYEGTINSIIQDYNKNFENPILPNCKIEWVTPENQKNILSEGEA
                     IICLSFDKKDHNLNFYNATLNFVQTGLIAKAKDYLNKSSSKAIDLLTTHIILRNNRKE
                     VLTTFRKKLTEFDDETRMEFETLVPTHDKGLFLNILLPEFHFYGELIDTLPPSSEYNT
                     EANGLFHWFKELATREFDEKTNLKFISKNLKVGVILVGKDETWETQGAAAYTKWADYY
                     ATENYNSVYVLARGDIGYERATTVTKILTHSKGFDQINKNPKIKCVSAEGQEYIVTCY
                     SLRPNKATIAYLAWEKFKEHYANGNLVPAIIDSVQKETIVINAFGLRFELPINLLSDI
                     EISDARKVFKVEDELYLTIAEFDSDRQHIVLSNKGTVSDPKHFIEAVLNANKVYTCKV
                     ERVQVDKQGLQSGLKVSTPELKHWIYIPKFKATPSRFLDLTTKFPAGAEVRVIIDNYS
                     SFSSNFVGYLENLTDPWESSTFKKLQPHDILNVTVKQINEFSVICEIEEGLECRFSRQ
                     EISWKSEECNTSKFKVDDKIKVIVIAIDTDKKRIDVSIKRLSKTPELEYFDANKNKVV
                     EVEIAKIVPEKGLAVKYPGSTNTGFIHWFEIGHGSVGRFENIYKQGDKIKAVVSEFDS
                     EKNSLKFSIKRQFTHQFDEWAAAIDDNSPLKGKVIGYFENAAHIELTQNGFTVQGFIL
                     RKYVSNYAFVESEDLPFYLPIGDSFHFYIFEINEDRKTISLARTEYLEQSGKPNYGEK
                     IKVKYVKENHSKGYFYSDELEGWTSLPEKDIKLGSTIEVIPISHSTGEYKIV"
     gene            complement(4972..5211)
                     /locus_tag="PLD44_03490"
     CDS             complement(4972..5211)
                     /locus_tag="PLD44_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98436.1"
                     /translation="MKEKNKIPTVFKEDLKKLLQSINEMEPIENGERLCKICSKVISL
                     ENIQLIIPRQHNTFDFICDSPVCLEEYNRKKEIKK"
     gene            complement(5402..6217)
                     /locus_tag="PLD44_03495"
     CDS             complement(5402..6217)
                     /locus_tag="PLD44_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOF98437.1"
                     /translation="MNTYLFLWNPKKWTWTTFEQDIEQVDLTGRCSQRWSCGNTKSIQ
                     PGDRIFLLKVGTEPKGIIAAGFATSTPFYERHWSGENKDTLYIDVDFEVLLNPDQEPI
                     LTVDILNTRNLAKQNWRPMGSGNSIKPGIVDELEAVWFDFLTTQKIRHNPFIPADNEV
                     QKTYTEGTPNQVSVTKYERNPFARKKCLEHYGFTCAVCDFNFEKTYGQIGKDFIHVHH
                     LTQVATVGQTYEIDPIKDLRPVCPNCHAIIHRQRTPLTIEEVKKLINDNADRK"
     gene            complement(6426..6557)
                     /locus_tag="PLD44_03500"
     CDS             complement(6426..6557)
                     /locus_tag="PLD44_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98438.1"
                     /translation="MGIKPGPKRITKTTGKPDKRQRDNKETPGNTPSLKPHKHKKGD"
     gene            complement(6878..7228)
                     /locus_tag="PLD44_03505"
     CDS             complement(6878..7228)
                     /locus_tag="PLD44_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98439.1"
                     /translation="MRIPLCAKLIESMCKWLVDNGFEDTPLHFSRFFPTNQLPDLPLT
                     PIKTLLAAREIAQAAGMKYVYVGNVPALGYENTFCPFCKKMLIERTGFTVNKNFINEG
                     KCMFCHADIPGVWQ"
     gene            complement(7331..8704)
                     /gene="xylE"
                     /locus_tag="PLD44_03510"
     CDS             complement(7331..8704)
                     /gene="xylE"
                     /locus_tag="PLD44_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HOF98440.1"
                     /translation="MNKKQENKVFVFGITLVATLGGLLFGYDTAVISGAEKALQAYFH
                     TSNLIHGITTSSALIGCIIGGSVSGVFASKLGRRKSLQVASVLFFLSALGSGYPEFLF
                     FKQGSASLDVLIAFNIYRIMGGIGVGLASAISPMYIAEIAPADIRGKLVSWNQFAIIF
                     GMLVVYFVNYSIAVGQSIEWINVIGWRRMFASEAIPASLFGILLFFVPETPRYLVLNN
                     KEDKAFNVLKKISGIDEGSKILEDIKATVHETTGKVLSYGWKILIVGILLSVFQQFVG
                     INVVLYYAPRIFQSMGAARDASMMQTVVMGFVNIVFTLLAILTVDKFGRKPLLIIGSI
                     GMAVAMFAISGLAYAEIIGVSTLVFIIIYTASFMMSWGPICWVLISEIFPNTIRSKAV
                     AIAVAAQWIANFLVSSTFPSLINFDPAFTYALYGCMAVLSAIFVWRMVPETKGKTLEE
                     MSKLWKK"
     gene            complement(8765..10081)
                     /gene="xylA"
                     /locus_tag="PLD44_03515"
     CDS             complement(8765..10081)
                     /gene="xylA"
                     /locus_tag="PLD44_03515"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOF98441.1"
                     /translation="MTTKEYFPTVGKIKFEGKESKNPFSFRYYDAEKVVYGRKMKDWF
                     KFSMAYWHTLCAEGSDQFGGPTKSFPWNEGKDALERAKNKMDAGFEFMQKVGINYYCF
                     HDIDLIDEGNSIEEYEANMKAIVEYAKQKQAETGIKLLWGTANVFSHKRYMNGAATNP
                     DFNAVARAAVQIKNALDATIALGGENYVFWGGREGYMSLLNTQMKREKEHLAMMLTMA
                     RDYARSKGFKGTFLIEPKPMEPTKHQYDVDAETVIGFLRAHGLDKDFKLNIEVNHATL
                     AGHTFEHELQCAADAGLLGSIDANRGDYQNGWDTDQFPIDIYDLTQAMLVILQNGGLQ
                     GGGTNFDAKTRRNSTDLEDIFIAHIAGMDAFARALESAAAILEKSPYKKMLTDRYASF
                     DSGKGKEFEEGKLTLEDLRTYALSKGEPALMSGKQELYEAIVNMYI"
     gene            complement(10173..11651)
                     /locus_tag="PLD44_03520"
     CDS             complement(10173..11651)
                     /locus_tag="PLD44_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOF98442.1"
                     /translation="MYLLGYDIGSSSIKASLINATTGECVSSEFFPKKEMQIKSVKPG
                     WAEQDPETWWENLKLATQAVITDSKVRSEEIKAIGISYQMHGLVLVDERLQVIRDAII
                     WCDSRAVPYGEKAFKEIGEEKCLSRLLNSPGNFTLAKLAWVKENEPENFKRIKYYMLP
                     GDYIALRLTGKPCTTASGLSEGIAWDFTKEAPADFLFDYFGFSTSIIPEIVPTFGNQG
                     FLSAEAAHELGLAAGTAVTYRAGDQPNNALSLKVLNPGEIAATAGTSGVVYGVNGEIK
                     YDPQSRVNSFAHVNHSASEKRIGVLLCINGTAILNAWIKNNVAPEGVNYNEMNEMAAS
                     IPIGSGGVSIIPFGNGAERMLGNREPNCSIHGINFNHIGKAHLIRAAHEGIAFSFKYG
                     MDIMNEIGIETKTIRAGHANLFLSPVFRQTLANITGATIELYNTDGSIGAARGAGIGA
                     EIYKNEEEAFATLKKILTVQPEKENQEITREAYDRWLQHLKF"
     gene            complement(11696..12406)
                     /locus_tag="PLD44_03525"
     CDS             complement(11696..12406)
                     /locus_tag="PLD44_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOF98443.1"
                     /translation="MESVNVYNPHVSVDCVVFGFDGEKLKVLLIERTIDEPDEQYNDK
                     KLPGSIIYENENLDEAASRVLGELTGLKNIYLSQFKSFGDPKRTSNPRDIYWLENTTK
                     MKIGRIVTVGYVALIKINRKIKLEEDNKTVQWYDVKDIDKINLAFDHNEIIHAALEHI
                     RHTLKVEPRLMFELLPRKFTMSQLRILYDVIHQTHSDVRNFQKKMMQLHQLVMLDEVE
                     KNVPHRAARLYKFDRKKL"
     gene            complement(13065..13940)
                     /locus_tag="PLD44_03530"
     CDS             complement(13065..13940)
                     /locus_tag="PLD44_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOF98444.1"
                     /translation="MYKIIVVLVGFMSFFSSVQGQLLWKISGNGLKKSSYLFGTHHFI
                     PLQFLDSIPGLYQAFNTSEIVVGEMVVNNLDAVEKVKKAAIIPNQQTIYDLVDKNQIL
                     QIDSALKSVLHIGLKEMALLQPNLILTIYQTEVYKKASGQIENIAPDSYFQQLGIAKG
                     KKIVGLETIDQQLEILFDKTSLQRQTEIFTYSVLHTDNMILETNKLNNFYKKGDLEKL
                     LLLSIQKNDPSDATDEEYYKLVDERNLEWMKQLPDLIKTSSCFITVGALHLPGENGLI
                     EQFRKLGYHVTEVKK"
     gene            14165..16402
                     /locus_tag="PLD44_03535"
     CDS             14165..16402
                     /locus_tag="PLD44_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOF98445.1"
                     /translation="MSNIYLIIILVLLGLAVLDLVVGVSNDAVNFLNSSIGAKVAPLW
                     IILTVASAGILLGSLFSGGMMEIARSGVFFPAKFTFPEVMMLFLAVMITDVILLDVFN
                     TFGLPTSTTVSLVFELLGSAVAVSLFKIWHSDAGAALSEYINAGKSITIISGIFISIA
                     IAFLCGTVIMYISRIIFSFNYKASFNYFGALWSGLAFTGIAYFVIFKVLLTSTLVSSE
                     TAAYINANIGTFLWGIFLASTVILGLLQHLFKINILKIVVLAGTMSLAMSFAGNDLVN
                     FTGPFMAALNSFQIASGVAASGGSIDNLYMGMLSEPVMADWRYLTASGIIMILALWFS
                     KKAKTVTKTEINLARQGEGIERFSSTQASRTLVRRAINLSRLIEKITPKKVKDFIESR
                     FEVVPLNDKDAPSFDLIRASVNLTVSALLISLGTSLKLPLSTTYVTFMVAMGTSLADR
                     AWGRESAVYRITGVLTVIGGWLMTALVAFTVSLIVGLTLMYGKVYAVYGLLLLVLILL
                     IQFASVHRKREKKEAAKSSTILTNEDAILHECTELVKRTIETTIRIFRETIDALHTED
                     RKKLRNLYKEADNLNNEWKDKRNYEVLPTLQHLQSGALEIGQYYVQIVDFSHEISKSL
                     RVITEASHKYIENNHEPFSQEQLNDLMTVCDAFVDVFNEYAKMVETNNFTSYANIRDK
                     QLRISELFSELTKKQIKRVKANESGTRNSILFLNILNEAKLISLQINNLMRARFRLFS
                     LSDKN"
     gene            complement(16508..16954)
                     /locus_tag="PLD44_03540"
     CDS             complement(16508..16954)
                     /locus_tag="PLD44_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOF98446.1"
                     /translation="MEKSKLMNEKQKLLDMRYMRMARIWAENSYCRRRQVGALLVKNK
                     MIISDGYNGTPAGFENNCEDEDDNSKPYVLHAEANAITKVARSHNSSDGATLYVTASP
                     CMECAKLIIQAGIKRVVYGEHYRIMDGIELLQRAGIEVVYLGDDNI"
     gene            complement(16960..18327)
                     /locus_tag="PLD44_03545"
     CDS             complement(16960..18327)
                     /locus_tag="PLD44_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOF98447.1"
                     /translation="MQRVHFIAIGGAAMHNLAIALSKKNNYKITGSDDEIFEPSYSRL
                     KEHGLLPEKTGWFPERIHKGLNAVILGMHAREDNPELLKAKELKLKIYSFPEFLYQQT
                     RNKTRIVVGGSHGKTTTTAMILFVLNKLKIEADYMVGAQIEGFDNMVRLSYDSRIAVF
                     EGDEYLTSPLDPRPKFHLYHPHIAVLTGIAWDHMNVFPTFENYLEQFKKFTDLIELQG
                     RLIYFDGDENLNKIVQQLRRDIVPFPYHTPPYEVRNEITWLKIKNKEIPLKIFGEHNL
                     QNLTAAHIACHQIGISDDDFFTAISEFSGASNRLEKLTENNDTVVFKDFAHSPSKVKA
                     TIKAVRQQYPHRRLIACLELHTFSSLMEDFLPQYARCMDEADVAMVYYDPEIIEQKRL
                     KQFEPEQVKTSFGKADLQVFTNKNDLEKALKSLDYKNSALLLMSSGNFSGLNVNDIVN
                     EKISL"
     gene            18428..19270
                     /locus_tag="PLD44_03550"
     CDS             18428..19270
                     /locus_tag="PLD44_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF98448.1"
                     /translation="MKIHKIEAGFFDCDGGAIFGVVPKKVWQKRYPCNEENFCKLAMR
                     CLLIDTGEKLVLIDSGTGDKQLDYLKYYNFKGVVNFELALQQLGYSCGDVTDVILTHL
                     HFDHCGGCTYYDSDRKNVLLTFPNAVHWVGERQWINFLEPNVREGDSYFPENMLPVQQ
                     AGKLKLVKENSFLCPEIELRIFNGHTVGQLVSYIQYLGKTWIYVGDVIPVAPSIPLAW
                     ISAFDAYPVTSLEEKKILLQEAEEKHQILLFEHDAYTECCTVKKSNDNFRMDESFPFT
                     NPVK"
     gene            complement(19491..20924)
                     /locus_tag="PLD44_03555"
     CDS             complement(19491..20924)
                     /locus_tag="PLD44_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF98449.1"
                     /translation="MSNFRWSIVSLLFFATAINYLDRQVLSLTWKDFIAPEFHWTNTH
                     YGTITSLFSLFYAIGMLFAGRFIDKLDTKKGFLWAIGIWSLGAVLHAFCGIATSGIVA
                     GKWLVGFEGAKEAIATVNNVSMVLNVSVTLFIFARLVLAIGESGNFPASIKAVAEYFP
                     KKDRAFATSIFNAGSTVGALAAPVVIPVIAEKWGWEMSFIIIGALGFIWMGFWAFIYK
                     KPEVHPKVNKLELEYIHLDHADEAAVNPNANTDKEKKLSFKQCFKFKQTWAFAFGKFM
                     TDGVWWFYLFWTPAYLSSVYGLKSSDPRSQLAIFVLYAITLLSIIGGWLPTYFVDKKG
                     MHPYAGRMKAMLIFAFFPLLALFAQPLGHISYWLPIIIIGIAGAAHQSWSANIFSTVS
                     DMFPKSAVATVTGIGGMAGGIGSMLINKASGALFDFAGQTQMSFLIFKGEEAGYFIVF
                     SFCAVAYLIGWIVMKALVPKYSPITDL"
     gene            complement(21002..21679)
                     /locus_tag="PLD44_03560"
     CDS             complement(21002..21679)
                     /locus_tag="PLD44_03560"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOF98450.1"
                     /translation="MMKFNKLEVLSSIKFTGVVPVFYHSNPEIAKSVVKACYEGGIRA
                     FEFTNRGEFAHDVFGEVVKYVQKNCPDMIMGVGSAIDAPTAALYIQLGANFVVGPLFN
                     PDVAKICNRRLVPYMPGCGSVSEIGFAQEAGCDVCKVFPGEVLGPAFVKALKAPMPWS
                     NLMVTGGVKPEEANLKSWFDAGVMCVGMGSNLFPADVIAAHDWGKITELCRETLDLIK
                     KIQKNIK"
     gene            complement(21724..22767)
                     /locus_tag="PLD44_03565"
     CDS             complement(21724..22767)
                     /locus_tag="PLD44_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOF98451.1"
                     /translation="MSKKIVTFGEVMLRLATPGYERFSQATEFSATFGGGEANVAVSL
                     ANYGMETEFVTRLPQNEIAESCIMDLRKHNVGTHYILRGGERMGIYFLETGAVARGSK
                     VIYDRAHSSIAEIEPGMIDWREVLKDADWFHWTGITPAISQGTADVCLEAVKVANELG
                     VTVSTDLNYRKNLWKYGKSASEIMPELVEGCDIILGNEEDAEKVFGIKPEGFDVSRTG
                     GEIDSAEFESVCKQLMKKFPRAQKVIITLRGSINANHNTWGGVLYSGEKLYQSKRYDI
                     THIVDRVGGGDSFMGGLIYGLLTYEGNDQKALDFAVAASCLKHTIYGDYNLVTIKEVE
                     ALMSGDESGRVSR"
     gene            23169..24245
                     /locus_tag="PLD44_03570"
     CDS             23169..24245
                     /locus_tag="PLD44_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOF98452.1"
                     /translation="MEKAKGKIRIKDIARMAGVSEGTVDRVLHQRGVVSEKSRKAVKK
                     VLEEIDYTPNLLARSLASKKKYRFICLFPAYNKGEYWESIEKGFRQASEEFNNYKVEV
                     MIEYFDQFDSSSFLATAKAVFVYNPDAIIMAPVFRQETILFARELTEKKIPFSFIDSM
                     VENVEFTSYYGQHSLQSGYIAGKLLFNSLPKESKILVLHTYRKGGSGSIQTENRKKGF
                     LQYIEENNLKNFCEILSIEIIEGNDEENMRLIRNAFIQHPDIRAAITFNSKVHRLAKY
                     FKNLSHSDIKLIGYDVLEENVKCMKEGFVSFLIAQRPEKQAYCAVRDMSFHLIFDKEV
                     NKINYVPIDILMKENIDYYLNFNE"
     gene            24417..>25366
                     /gene="amrS"
                     /locus_tag="PLD44_03575"
     CDS             24417..>25366
                     /gene="amrS"
                     /locus_tag="PLD44_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOF98453.1"
                     /translation="MNTSIASDWIQPALYQQPLENGMVKCVLCPHQCMVHEGKTGLCH
                     TRQNINGVLFTLAYANPCSLNVDPIEKKPLFHFLPGSKIFSLATAGCNFKCLNCQNWE
                     ISQSSPQEVIHYDLLPEAVVELASNRQIASIAFTYTEPTVFYEYVYDTAKLAHQKNMK
                     TVIVSNGYINEKPLLDLCPFLDAANIDLKCFDNHLYRKLTGGSLEPVLKTLKILKEQG
                     VWLEITNLIVPTYSDAPEMIENMCKWLVDNGFEDIPLHFSRFFPANQLPDLPPTPIKT
                     LLAAREIAQTAGMKYVYVGNVPALGYENTFCPSCKKLLIER"
BASE COUNT         7626 a         4927 c         4457 g         8356 t
ORIGIN      
        1 aattttgaaa aaattaaaaa attatctttt gctgccccgc ataaattctt cttgtagtaa
       61 gtttataaaa ctgtttattg taaaaaatca tttcttccta cttctccaca cgatgcacat
      121 tgtgcgggag agaggggtag taagtctata aaacgttttc gggctttgcg ttctggcagg
      181 ttttcggagc acttcactgt caagccacga tgaagtttga agcgagccaa agccgttgat
      241 ttcagcacta tctcggcaat aaaatatacc gcctgttaac agccgtagtt ctatattatt
      301 ccgttttctt ttgctatttt cacacaataa gttttatcct tgcaattttc aagtatatca
      361 taattatttt taaaaaactc tattgtgcca agtcctttac tttttgtgcc aaaatattca
      421 atcaatacgg ttggaacaaa atacaaatca aatgtattat ccgcattagg tctaaagcca
      481 acaattacat ctgaagtttc cggtgattgt gtataagttt tcacatctga tttatactct
      541 ctgtcaactc ccccacgtga accaccagta aagcttacgc ttgttcttgc tgttttaact
      601 tgtactctta ttatatcttc ttttccattt gcaagtttac gaactattat aatgtcatat
      661 ggggagagtg gtaaatcaac cagtgaaaca ttttgatacc gtttcatcaa aatccctaca
      721 acaatgtgtt catgtgcaaa accatcaatt gatgcttgtt tgcctatttc ttcgttattt
      781 ggcatattac ttttttaaaa ctataatcca atctgtattg attctttctt ctcttggaac
      841 tttaatgaaa tgttttatta tttcagaacc aaaataattt tcaacttgat aaccaatatt
      901 ttttgaaaga ctcattaaat atttccaact ttcaaataat tctcctttga tacgattatt
      961 accaacaaca ataatatatc tgcctcctgg ttttaatact ctatacactt ccttcatatt
     1021 taaagtcata tcgtataagt acttatatgc tatatatgcc cttcttgcgt caagtttata
     1081 gattgcttct attttcttgt cggcatattc ctctccaatt ctatgtagtt gtttatattc
     1141 attaattctc actacttctg taccgatatg ggtttctttt agtttactca atgaaccact
     1201 tgctaatcca agccagtaca tttctaattg gtgtgttctt ggataatcaa cggcatttac
     1261 ataaggagga gaagttattg cgaggtcaaa atgattgtct ggatatgtta aaaaccttgc
     1321 atcatttcca tcaggtattt caaccgtggc attagatttt agtcgatttg taaaatcatt
     1381 aattctatat gaatttacta ttaaattttc tgcaaatttt gtcaacgcaa gagaagggta
     1441 tatttccttt tttaattttt ttcttacgac agttcttgta caattatcgt ctgcattaga
     1501 aacagcacga ataatggatg acatagaaat taaacataaa tctttaatat ttttatcgtt
     1561 tgttgagttg agaattattt tttttataaa tgccaattca agtaatattt ctctagcaaa
     1621 ccagttctcc cgataaggaa attcaggaat atccttattt tctaccagag taggattata
     1681 agtaacaagt tgtttaagaa tcctatcaac agttttattg agttcaagtt tgtcaatagg
     1741 agttgtttta acttttgcaa gatatttagc aaatggatca atatcaattg caacagaatt
     1801 tctgtttaat agtagtgctt caatatttgt agtggcacta cctgcaaatg ggtcaagtat
     1861 caagtcattt ggctttgaat agcgattaat taaccatctt ggtagttcag gaaaaaattt
     1921 tgccggatat ttatgaattc cgtgtgaata tttagattgg tcataactta taaacaaaaa
     1981 tctttgacca tctgttaatg gaaggtctgc cggtatgtct ccatcaactc taataagagt
     2041 ttctccaaat tcatttgtaa tttcctttat atttgagaga cctccaaatt gcgaatattg
     2101 aacaggttgc ttgaatattt tctttttagc aactttctct ttaatttctt ttattgaaat
     2161 tggataataa ttcatcttaa agtcatctta aagttttttc aaaaataata atcttttctt
     2221 atattttctc tttagatgtc aaattttatt gactgtctct tttactatgg ctggtaacgt
     2281 tttcgggctt tgcgttcggg agggtttcgg accacaaaac tgtcaaccat cactgaactt
     2341 gaatagaaac acaaagctcc aagtttgcac gtcaccccgc atgacgccaa aacccgtgtt
     2401 accagctggc attcttgttt tatcagacaa ttttatactc acctgtcgaa tgtgaaattg
     2461 gtattacttc tatggttgaa cccaacttaa tatctttttc aggaagacta gtccagcctt
     2521 ccaattcgtc tgaatagaag tagccttttg agtggttctc ttttacatat tttactttta
     2581 ttttttcgcc ataatttggc ttcccagatt gttccaaata ttctgtcctt gccaatgaaa
     2641 tggttttacg gtcttcattt atttcaaaaa tgtagaaatg aaaactatct ccaattggca
     2701 ggtaaaaagg taaatcttca ctttccacaa atgcataatt tgaaacatac tttctcaaaa
     2761 taaatccctg aacggtaaag ccattttgag ttaattcaat atgtgcagca ttttcaaaat
     2821 agccaattac tttccctttt aatggggaat tatcatcaat tgctgctgcc cattcatcaa
     2881 attgatgagt gaattgtctt ttaatactaa actttaaact gtttttttca gaatcaaatt
     2941 ctgatactac ggcttttatt ttatcacctt gtttgtaaat gttttcaaac ctacccacag
     3001 aaccgtgacc aatttcaaac caatgaataa atccagtatt tgtgctgcct ggatatttga
     3061 ctgctaaacc tttttcggga acaattttag caatttctac ttcaacgact ttgtttttat
     3121 ttgcatcaaa atattccaat tcaggagttt tggaaagtcg tttaatcgaa acatcaattc
     3181 gctttttgtc ggtgtcaatt gcaatgacaa ttactttaat cttatcgtca actttaaatt
     3241 tggatgtgtt acattcctca gatttccaag aaatttcctg cctagaaaag cgacattcaa
     3301 ggccttcttc aatctcacag attacagaaa attcatttat ctgtttaact gtcacattta
     3361 aaatatcatg tggttgtaat tttttaaaag tactgctttc ccaagggtca gttaaatttt
     3421 ctaaatagcc cacgaaattg gaagaaaagg aagagtagtt atcaattatc acacgcactt
     3481 ctgcccctgc tggaaacttg gttgtaaggt caagaaatct actcggtgtc gctttaaatt
     3541 ttggaatata aatccaatgc ttcaattcgg gtgtagaaac tttcaacccg ctttgcaatc
     3601 cctgtttatc aacttgaact ctttcaactt tacaagtgta aactttattt gcatttaata
     3661 cggcttcaat aaaatgctta gggtcagaga ctgttccttt gttggagaga acaatatgtt
     3721 gtctgtcact gtcaaactct gcaatggtca aataaagttc atcttcgact ttgaagactt
     3781 tacgtgcatc agaaatttca atgtcagaaa gcaaattgat aggaagttca aatctcaatc
     3841 cgaatgcatt tataactatt gtttcctttt gaacactatc aataatggca ggtactaaat
     3901 tcccgtttgc atagtgttct ttgaattttt cccaagctaa atatgcaatt gtagctttat
     3961 tgggtcgtag agagtaacaa gtaacaatat attcttgacc ttctgccgaa acacatttga
     4021 tttttgggtt tttatttatt tggtcaaatc cttttgaatg ggttaggatt ttggtaactg
     4081 ttgttgctct ttcataccca atgtctcctc ttgccaaaac ataaactgaa ttgtaatttt
     4141 cagtagcata atagtcagcc cattttgtat atgctgctgc accttgggtt tcccaagttt
     4201 catcttttcc aacgagtatt actccaacct ttaagttctt ggaaataaat tttagattgg
     4261 ttttttcgtc aaattctctt gttgccaatt ccttgaacca atgaaataag ccattcgctt
     4321 ctgtattgta ttctgaactt ggaggtaaag tgtctatgag ttcaccgtaa aaatgaaatt
     4381 cgggtaaaag aatgtttaag aatagtcctt tgtcgtgtgt tggtacaagt gtttcaaact
     4441 ccattcttgt ctcatcatca aattcagtta gttttttacg gaatgttgta aggacttctt
     4501 tgcggttgtt tcttaaaata atgtgagttg taagcaagtc aattgccttg gaggatgact
     4561 tgttcaaata gtcttttgct ttggcgatta aaccagtttg aacaaagttt aaagtggcat
     4621 tataaaagtt aagattatgg tctttcttat caaagctcaa acaaatgatt gcttcgcctt
     4681 cactcaggat gtttttctga ttttctggtg taacccattc tattttgcaa ttaggcaaaa
     4741 ttgggttttc aaagttttta ttgtagtctt gaattattga attgatagtt ccttcatact
     4801 cgcttttcac actcagtttt cttacactac ttccaaccca gccaaagaaa ttgagtatgt
     4861 ctcctaaaac aactttacct ttagcccaaa cctcaccgcc taaaaatact cctgctaaaa
     4921 ccaataaaac tgaaatcgga atgtaaatga gtatcaattc aaccaattta ttcatttctt
     4981 tatttctttt ttacggttat actcttccaa acaaacagga ctgtcacaga tgaaatcaaa
     5041 agtgttgtgc tgtcttggaa taattaactg gatattttcc aatgaaataa ccttagaaca
     5101 aatcttgcag agtctttccc cattttcaat tggttccatt tcgttgattg actggagcag
     5161 tttttttaag tcttccttaa aaacagtagg tattttattt ttctctttca ttgaaattta
     5221 ttttcttatt tatctacagt agcagtcttt aacgcttgct ggtaacgttt tgcagctttg
     5281 cgttcgtgcg agatttcagg gcacaaaact atcaataagc actgaacttg aattgaagca
     5341 caaagctcca agtttgcacg tcagcccgcc ggacgcaaaa cctgtgttat gggctgatgt
     5401 tctattttct gtctgcattg tcgttaatta atttttttac ttcttcaata gtcaacggag
     5461 ttctttgtct atgaattatt gcatggcagt tcggacaaac aggtcttaaa tcttttatcg
     5521 ggtcaatttc ataagtctgt ccaaccgttg caacttgcgt caaatggtga acgtgaataa
     5581 agtctttccc aatttgtccg tatgtttttt caaagttgaa gtcgcaaaca gcacaagtaa
     5641 aaccgtaatg ctctaaacac tttttccttg caaatgggtt tcgttcatat tttgttactg
     5701 aaacttggtt tggtgttcct tctgtgtatg ttttttgaac ttcgttgtcg gcaggaatga
     5761 acggattgtg tctgattttt tgagttgtca agaagtcgaa ccaaactgct tcaagttcgt
     5821 caacaattcc aggttttatt gagttgcccg aacccattgg tcgccaattt tgtttggcta
     5881 aatttcgtgt gtttaaaatg tcaaccgtca agattggttc ttggtcaggg tttagtaaaa
     5941 cttcaaagtc aacgtctatg tatagagtgt ctttattttc tccactccaa tgtctttcat
     6001 aaaatggtgt cgaagttgca aaacctgctg caataattcc tttcggttct gtcccaactt
     6061 ttagtaaaaa aattctgtcc cccggttgaa ttgattttgt gttgccacaa ctccaacgtt
     6121 gagaacaccg tcctgttaag tccacttgtt caatgtcttg ctcaaatgtc gtccaagtcc
     6181 actttttagg gttccataaa aatagatatg tgttcattcg tttttgtctg tcgttttaca
     6241 cttgcccata acgttttgcg gtatgaaaag ttgccgattg cgggcgattt cctgtcaagt
     6301 tacacaaaag ttgaagcggg ctacaaccct tgaataacat actgaaccgg caatttttta
     6361 taccgcatgt tacagggcgt attttattag aaattttaat ccaaacattt ccacagtcgt
     6421 tggtcttagt ctcctttttt gtgtttgtgt ggtttcaatg aaggtgtatt tcccggagtt
     6481 tctttgttgt cgcgttgtcg cttgtccggt tttcctgttg tttttgtaat tctttttggt
     6541 cctggtttaa ttcccataat ttttcctcct tagtttgttt tacattaggt aatttattat
     6601 tgatactcaa taattttatt tatcaaacct atttgatttt caatgaatta ctgcatcttt
     6661 taatatgccc tgtaacgtct tatttacgca ataaatatac aactttagta aatacaaaat
     6721 ttaaaacatg taataatcac ttttagcttg cttttatcat aaaatcccta caaaaataca
     6781 caacaatttt aattattcaa aacaaatgat taaaaaaatt aattatttgt ttaattgatt
     6841 gaattattgg agaataagaa gataaaattc attcgtttta ttgccaaacg cctggaatgt
     6901 cggcatgaca aaacatacat ttgccttcgt taatgaaatt tttgttaacg gtaaaaccgg
     6961 ttcgttctat cagcattttt ttgcagaaag gacagaaagt attttcgtag ccaagtgcag
     7021 gaacatttcc cacataaaca tatttcattc cggcagcttg ggcaatttct cgggctgcaa
     7081 gcaatgtttt tatgggtgtt agaggtaaat caggtaattg gttggtcggg aaaaatctac
     7141 tgaaatggag tggcgtatct tcaaatccat tatcaaccag ccatttgcac atgctttcta
     7201 ttaacttggc acatagagga attctcatcc tggcagttta ataccatgcc cgacacacaa
     7261 agaaaatcca ctccggagaa attcttttat ttccggagtg gaatcaatca caaaaaacca
     7321 actaaattca ctattttttc cagagtttgc tcatttcttc cagcgttttt cctttggttt
     7381 cgggcaccat tcgccaaaca aatattgccg ataaaactgc catacatcca tacaaagcat
     7441 aagtaaatgc cggatcaaaa ttgatcaaag acgggaaagt ggaagaaacc aggaaattgg
     7501 caatccattg agctgcaacg gcaatagcaa ccgctttgct acgaatggta ttggggaaaa
     7561 tttccgaaat cagtacccag caaattggtc cccacgacat cataaaggat gccgtataaa
     7621 taataatgaa aaccaatgta ctgacgccga taatttctgc ataagccagt ccgctaatgg
     7681 caaacatggc aacagccata ccaatggaac caataatcaa caaaggtttt ctgccaaatt
     7741 tatctactgt cagaatggct aacaacgtaa atacaatatt tacaaagccc attacaactg
     7801 tctgcatcat ggaagcatcg cgggcagcgc ccatactttg aaaaattcgc ggagcataat
     7861 acaataccac atttatacct acaaattgct gaaaaacaga tagaagaata ccgactatca
     7921 aaattttcca gccgtacgaa agaacttttc cagtagtttc gtgtactgtt gccttgatat
     7981 cttccaaaat ctttgaacct tcgtctatac ccgatatttt tttcagcacg ttaaatgctt
     8041 tgtcttcttt attgttaagt accaaataac gaggtgtttc agggacaaaa aacagcaaaa
     8101 ttccaaaaag cgatgcagga atggcttcag aagcgaacat ccttcgccag cctatgacat
     8161 tgatccattc aatggattga ccaacagcga tggaataatt cacaaaataa accaccagca
     8221 ttccaaaaat aatggcaaac tgattccatg aaacgagttt tccccgaata tcggcaggag
     8281 ctatttcggc aatgtacatg ggagaaatag ccgaagccaa ccctacgcct attccaccca
     8341 tgatgcgata aatattaaaa gcaattaaca catcaagcga agcactacct tgcttgaaaa
     8401 acaaaaattc cggataacct gagcccaatg ctgataagaa aaacaaaact gaagctactt
     8461 gcaaagactt tcttcgtcca agttttgatg caaaaactcc ggagaccgat cctccaataa
     8521 tacaacctat gagtgcactt gaagtagtaa taccatgaat taaattgctg gtgtgaaaat
     8581 atgcttgcaa agctttttca gctcctgaaa tcacagccgt atcatatccg aagagcaaac
     8641 cacccaacgt ggctaccaat gttattccaa aaacaaaaac cttgttttct tgtttcttat
     8701 tcataattat aaatttagcc atttaagcac tttcaaaaag taacttgaat ggggctgatg
     8761 aattttaaat atacatatta acaatagctt catacaattc ttgcttgcca ctcataagag
     8821 caggttcgcc tttggaaagt gcataagtgc gcagatcttc cagtgtcaat ttgccctctt
     8881 caaattcttt accttttcca ctatcaaaag aagcataacg atcagtgagc atttttttgt
     8941 agggcgattt ttccagaatg gcagcagccg attccaatgc acgtgcaaaa gcatccatcc
     9001 cggcaatatg agcaataaaa atatcttcca aatcagtaga attacgacgg gttttggcat
     9061 cgaaattcgt tcctccaccc tgaagaccgc cgttttgcaa aataaccagc atggcttgtg
     9121 tcaaatcata aatatcaatc gggaactgat cggtatccca accgttttga taatctccac
     9181 gattggcatc aatcgagccc agtaaaccag catcggcagc acattgcaat tcatgttcaa
     9241 aagtatgacc ggcaagggtt gcatggttca cttcgatatt cagtttaaaa tctttatcca
     9301 gaccgtgagc acgcagaaaa ccaataaccg tttcagcatc tacatcatat tggtgtttgg
     9361 tgggttccat tggtttgggt tcgatcagaa aagttccttt aaaacctttt gagcgcgcat
     9421 aatcgcgtgc catagtcaac atcatggcca aatgttcctt ttcacgcttc atttgcgtat
     9481 tgagcaagct catgtaacct tcacgtccac cccagaatac ataattttcg ccaccaagag
     9541 caatcgtggc atccagcgca ttcttgatct gaacggctgc ccgtgctaca gcattaaaat
     9601 caggattggt agcagctccg ttcatatatc gtttgtgcga aaatacgttg gctgttcccc
     9661 aaagcaactt gatccccgtt tcagcctgtt tttgcttcgc atattccaca atggctttca
     9721 tgttggcttc atattcttca atggaatttc cttcatcaat caaatcgata tcatggaaac
     9781 aataataatt gataccaacc ttttgcatga attcaaaacc agcatccatt ttgtttttgg
     9841 ctctttccaa tgcatctttt ccttcattcc acggaaaact tttggtgggt ccgccaaact
     9901 ggtcgcttcc ttctgcacac aatgtatgcc agtatgccat tgaaaattta aaccaatctt
     9961 tcattttacg gccatacact actttttcgg catcgtagta ccgaaacgaa aaaggatttt
    10021 tactctcttt tccttcaaat ttgatttttc ctactgttgg aaaatactct tttgttgtca
    10081 taattcataa attattaaat tcctcagcca aattagctta agaataagtt aaacattttt
    10141 tattatcttg taattatcac taaaatttct atttaaaatt tcaaatgctg caaccatcga
    10201 tcgtaagctt cacgcgtaat ttcctgattt tctttttcag gctgaacggt aagaattttc
    10261 tttaacgttg caaaagcttc ttcttcgttt ttataaattt ccgctcctat tcctgctccg
    10321 cgagcagcac ctatcgagcc atcagtatta tacaactcaa tggttgcacc ggttatgtta
    10381 gccagtgtct gacggaaaac gggcgaaagg aaaaggttgg catgtccggc acgaatggtt
    10441 ttcgtctcga tacctatttc attcataata tccattccgt atttaaacga aaatgcaatg
    10501 ccttcatgag cagctcttat taaatgagct ttgccgatat gattgaaatt gatgccatga
    10561 atggaacaat tgggttcgcg atttcccaac atccgttctg ctccattacc aaaaggaata
    10621 atacttacgc cgccactgcc aataggaata cttgcagcca tttcattcat ttcattatag
    10681 ttcacacctt caggagccac gttatttttt atccatgcat tcaatatagc tgtaccatta
    10741 atacaaagca gcacaccaat acgcttttca ctggctgaat ggttaacatg ggcaaacgaa
    10801 ttgactctcg actgcggatc gtattttatt tctccgttca caccataaac aacgcctgaa
    10861 gttccggcag tagcagcaat ttcacccgga tttagcacct ttaacgacaa ggcattgttt
    10921 ggttgatcgc cggcacgata agtaacggct gttcctgctg caagtccaag ttcatgcgca
    10981 gcttctgcac tgaggaatcc ctgattgcca aaagttggaa caatttcagg aataatggaa
    11041 gtactaaacc caaaataatc aaacagaaaa tcggcaggag cttcctttgt gaaatcccat
    11101 gctattcctt ccgacaagcc cgatgcagta gtacagggct ttcctgtcag tcgtaaagca
    11161 atataatcgc caggcaacat gtaatacttg attcgtttga aattttccgg ttcattttct
    11221 tttacccaag ccagttttgc aagcgtaaaa tttcccggag aattcaataa gcgagataag
    11281 catttttctt ctccaatttc cttgaacgct ttttctccgt aaggtacagc acgactgtcg
    11341 caccagataa ttgcatcccg aattacttgc agtcgttcgt ccaccaatac caaaccatgc
    11401 atctgatagg aaatgccgat ggctttaatt tcttccgacc gaactttact gtctgttatt
    11461 acagcttgcg ttgcaagttt gagattttcc caccacgttt ccgggtcttg ttctgcccat
    11521 ccgggtttca ccgatttaat ctgcatttct ttcttcggga aaaattcact cgaaacacat
    11581 tcgcctgttg tggcattaat aagacttgcc ttaatggagg aacttcctat atcatatcca
    11641 agtaaataca taattcttag tcgattattg atttataata atgatttttt cagatttata
    11701 atttctttct atcaaattta tacaaccgtg cagcacggtg aggcacattt ttttctactt
    11761 cgtcgagcat gaccagttga tgcaactgca tcattttttt ctgaaagttt ctcacatccg
    11821 aatgagtttg atgaataaca tcatagagaa ttcttaattg tgacatggta aattttcggg
    11881 gcagcaattc aaacataaga cgaggctcta ctttcaaagt atgccgaata tgttccaaag
    11941 ccgcatgaat aatttcattg tggtcgaaag caagattaat tttgtcgata tctttcacat
    12001 cgtaccactg caccgttttg ttatcttctt ccagcttaat ttttctgttt attttgatta
    12061 aagccacata gccaaccgtt acaatacgcc cgattttcat tttcgtggta ttttcaagcc
    12121 aataaatatc gcgtggatta ctggttctct tgggatcacc aaaactctta aactgactga
    12181 gataaatatt cttcaatccc gtgagttctc ctaaaacacg actggcagct tcgtccaaat
    12241 tttcattctc ataaataatg ctccccggca gttttttatc gttgtattgt tcatcgggtt
    12301 catcaatagt tcgctcaata agcaaaactt tcagtttctc tccatcaaat ccgaaaacca
    12361 cacagtcaac cgagacatgc ggattataaa catttactga ttccattgat ttataaagaa
    12421 gtttttgaat aacgacacaa aaataatatt tgattttaaa ataatagcat aagatattat
    12481 gttataaaac acatttacaa tatattaaac attgtatttt gtacaatatg taattgaatc
    12541 atattgtata aagtacaata ataccaatga aaacaacaaa atttatttat tgaaaaaaag
    12601 gtacagcaaa atatttcgtc tgtttattta aaagattttc agctttttac aatggaataa
    12661 taaaactttt ttctttttgg gagaaatacc gaaaaacttt tggggatagg aaaatttttc
    12721 tgaaaaaaca ataaaaagaa agagtcttct cttaaaaacc taattttttt aaagtttcaa
    12781 tcggctttaa tgtagtagac ataagctgcc aagacttggc aatgcgttct ttgatagatg
    12841 ataaatatcg attgtgagtt attgactttc gcatatacca ccaaacacgt tcaacaggat
    12901 tcaagtccgg agaatatgcc ggaagatatc ttatttccaa tgtgggatta gcttccagaa
    12961 agcccttaag cttttttgca tgatgaaacc tcacgttatc tactggattt acctttggac
    13021 aactgattgt gtaataattt aaggtaatat atataattgt tgcctcattt ttttacctct
    13081 gttacgtgat atcccaattt tctgaattgt tctatcaggc cgttttcgcc aggcaaatgc
    13141 agtgctccga ctgtaataaa acatgatgag gtttttatca ggtcaggtag ctgtttcatc
    13201 cattcaagat ttctttcatc cactaattta taatattctt catctgtagc atctgaagga
    13261 tcgttttttt gaatcgacaa taaaagcaat ttttctaaat cacccttttt gtaaaaatta
    13321 ttcaatttgt tggtttctaa aatcatgttg tcggtatgaa gaactgaata agtaaatatt
    13381 tctgtctgac gttgcaggga agttttgtca aaaagtattt ccagttgctg gtcgatggtt
    13441 tcgagtccta caattttttt tccttttgct attcctaact gttgaaaata cgaatcgggt
    13501 gcaatatttt caatttggcc ggaggctttt ttgtatactt cggtttgata aatggttaaa
    13561 atcaaattgg gctgcagcag ggccatttct ttcaatccta tgtgaagaac cgattttaga
    13621 gctgagtcga tttgcaagat ttgattttta tctactaagt cgtaaatagt ttgctggttg
    13681 ggaataatag ctgctttctt aactttttcc accgcatcca ggttgttcac caccatttct
    13741 ccaaccacaa tttcagaagt attaaatgct tggtataagc cagggatact atcgagaaat
    13801 tgtaacggaa taaaatgatg ggtgccaaac aaataagaac tttttttcaa tccgttgcct
    13861 gagattttcc ataacaactg tccttgaacg gatgaaaaaa acgacatgaa tcctaccaga
    13921 acaacaatta ttttatacat gaaatgttaa attatatggt tggcacaaat atatgcaaat
    13981 tatttgttga gataaggaaa caaaatcagg caactactgt tttttgtgtt ttttaattga
    14041 aattacgatt tctatcaatt cacatttgtg ttttgtaaca tttttgaaat atctttttaa
    14101 tggatttgaa atttgtgtta taaaaaacga tagttatctt tgtgcaaaat tattaatttc
    14161 taatatgagt aacatatacc tgattatcat tttagtattg ttaggattgg cggtgttgga
    14221 tttggtggtt ggagtatcga atgacgctgt taattttctt aattccagta ttggggccaa
    14281 agttgctccg ctttggataa ttcttactgt tgcttctgcc ggtatacttt tgggttcttt
    14341 attttcaggt ggtatgatgg aaattgccag aagtggcgtt tttttccctg caaagtttac
    14401 ttttcctgag gttatgatgt tgtttttggc cgttatgatt accgacgtaa tcctgctcga
    14461 cgtattcaat acttttggtt tacctacttc taccactgtt tctcttgtat ttgaattact
    14521 tggttcggct gttgcagttt ctttatttaa aatttggcac agtgatgcag gtgctgcatt
    14581 gtcagagtat attaatgcgg gcaagtcgat taccattatt tctggaatat ttatctccat
    14641 agccattgct tttttatgcg gaacagtaat tatgtacatt tcacgcatta ttttttcttt
    14701 caattataaa gcttctttca attattttgg tgcattatgg agtgggcttg cttttacggg
    14761 cattgcttac tttgttattt ttaaggtttt gttgacttct actttggtat cttcagaaac
    14821 ggctgcatat ataaatgcca atattggtac ttttttgtgg ggtatattct tagcgagtac
    14881 tgtaatttta ggacttttac aacacctttt taaaataaat attctcaaaa tagtagtact
    14941 ggctggtacc atgtcgttgg ctatgtcatt cgcaggcaat gatttggtga attttaccgg
    15001 tccttttatg gctgctttaa attcttttca aatagcttca ggtgtggcag caagcggagg
    15061 ttctattgat aatctttaca tgggtatgct tagtgaaccg gtaatggccg attggcgata
    15121 tttgactgct tctggtataa taatgattct tgccctgtgg ttttcaaaaa aagccaagac
    15181 tgttaccaaa acagaaatca acctggcaag gcagggcgaa ggaatagagc gtttttcttc
    15241 aactcaggct tcgcgtacac ttgtgagaag agctatcaat ttatcaagat tgatagaaaa
    15301 aattacaccc aaaaaggtca aagattttat tgagagtcgt tttgaagttg tacctttgaa
    15361 cgataaagat gctccctctt ttgacctcat cagggcttca gtaaatttaa ctgtttccgc
    15421 tttgcttatt tctttaggta catcattaaa gctacctctt tctactactt acgttacttt
    15481 tatggtggct atgggaactt cacttgccga tcgagcttgg ggaagagaaa gcgccgttta
    15541 tcgcattact ggggtactta cggttattgg tggctggtta atgactgctc tggtagcatt
    15601 taccgtttcc ttgatagtgg gattgaccct tatgtacgga aaagtttatg ctgtctacgg
    15661 attattgctt ctggttttaa ttttattaat tcagtttgct tctgttcata ggaaaagaga
    15721 gaaaaaagag gcagcaaaaa gttcaactat tttgactaac gaagacgcaa ttctgcatga
    15781 atgtaccgaa ttggtgaaac gaaccatcga aactactatt cggattttta gggaaaccat
    15841 tgatgcactt catacagaag acagaaaaaa gctcagaaat ctttacaagg aagcagacaa
    15901 cttgaataac gaatggaaag acaaaaggaa ctatgaagtt ttgcctaccc ttcagcatct
    15961 tcaatccggt gctttggaaa taggtcagta ttatgtgcag attgtcgatt tttcgcatga
    16021 aatatccaaa tcgttacgtg tcattaccga agcaagtcac aaatatatag aaaataatca
    16081 tgaacctttc agccaagaac aattaaacga cctgatgacc gtttgcgatg cttttgtaga
    16141 tgtttttaat gaatatgcaa aaatggttga aacaaacaat ttcaccagct atgctaatat
    16201 acgagataaa caactcagaa tttcggaatt gttttccgaa ctgacaaaaa aacaaattaa
    16261 aagagtcaaa gcgaatgaat cgggaacgcg taacagtata ttgtttttga acatactgaa
    16321 tgaggcgaag cttatttcgt tgcaaatcaa taacctgatg agagcccgtt ttagattgtt
    16381 ttctttaagt gataaaaatt aaagcagtat tttaatttgt cagtccgtcg tgccacagat
    16441 aggcttccgt ttatcgtaaa ggtttaaaat aatttttgta atccataata caaaaaagag
    16501 gtgaatttta tatgttatca tcgcccagat aaaccacctc aatgccggca cgttgtaaaa
    16561 gttcgatgcc atccatgatt cggtaatgtt cgccatacac aactcttttg atgcctgcct
    16621 gaattattaa tttggcgcat tccatgcagg gagaggcggt aacgtaaagc gttgcaccgt
    16681 cgctgctatt gtgcgaacgt gctacttttg ttatggcatt tgcttcagca tgaagcacgt
    16741 aaggttttga attgtcgtcc tcatcttcac agttattttc aaatcctgca ggtgtgccat
    16801 tgtagccatc cgaaataatc attttatttt tcaccagtaa agcacccacc tgcctgcgac
    16861 gacaatagga gttttctgcc catattctgg ccatgcgcat ataccgcata tccagcaatt
    16921 tttgtttttc attcataagt tttgattttt ccatctgttt cacaatgaaa ttttttcatt
    16981 gacaatgtca ttcacattca aacccgaaaa gtttcccgac gacatgagta aaagggccga
    17041 atttttataa tccaaagatt ttaaagcttt ttccaaatca ttcttattcg taaaaacctg
    17101 taagtctgct tttccgaaac ttgtttttac ctgttctggc tcaaattgtt tcagtcgttt
    17161 ttgttcaata atttcagggt cgtaataaac catagccaca tctgcttcgt ccatgcatct
    17221 tgcatattga ggaagaaaat cttccatcag actgctgaag gtgtgtagtt cgaggcaggc
    17281 tattaatctg cgatgaggat attgttgccg aacggctttg atggtagctt taactttgga
    17341 aggagagtgg gcaaaatcct tgaatacgac ggtatcattg ttttcggtca atttttccaa
    17401 tcgattagaa gcgccggaaa attcggaaat agccgtaaaa aaatcgtcgt cggaaatgcc
    17461 aatctgatga caggcaatgt gtgcggcagt caagttttgc aaattgtgtt ctccgaaaat
    17521 ttttaaagga atctccttgt ttttaatttt aagccaggta atttcgtttc ttacttcata
    17581 aggtggtgtg tgataaggaa aggggacgat gtcgcggcga agttgctgaa caattttatt
    17641 caggttttcg tctccatcaa aataaattag tcttccttgc aattcaatta aatcggtgaa
    17701 ttttttaaat tgttctaaat agttttcaaa agttggaaaa acattcatat gatcccaggc
    17761 aatgccggta agtactgcaa tatgtggatg atataaatga aatttagggc gtggatcgag
    17821 cggggaggta aggtactcgt ctccttcaaa aactgctatg cgcgaatcgt aagaaagcct
    17881 gaccatgtta tcgaaacctt caatttgagc gccaaccata taatcggctt caattttcag
    17941 tttgttcagc acgaataaaa tcatggctgt tgtggtggtt tttccgtgac ttccgcccac
    18001 cacaatacgc gttttgttgc gtgtctgctg ataaaggaat tcagggaaag aataaatttt
    18061 caattttaat tcttttgctt tcagcagttc aggattatct tcgcgggcgt gcatgccgag
    18121 aattacagca ttcaaaccct tgtggatgcg ctcgggaaac cagcccgttt tttccggcaa
    18181 cagtccatgt tctttcaatc gggaataaga aggttcaaaa atttcatcat ccgaacctgt
    18241 aattttatag ttgttttttt tactgagtgc gatggctaaa ttatgcatgg ctgctccgcc
    18301 aatggcaata aaatgaactc gctgcataag gttggaaggt tttttaaatt ttattgctac
    18361 ttttgtggaa agtatttttt ataatgcaaa aatacgaaga aaatacgaat cgaaagctac
    18421 tttgaatatg aaaatacata aaatagaagc cggttttttc gactgcgatg gtggagccat
    18481 ctttggtgta gttcccaaaa aggtttggca aaagcgttat ccgtgcaatg aggaaaattt
    18541 ttgtaagctg gccatgcgtt gcctgttgat cgatacaggc gaaaaacttg ttttgattga
    18601 cagtggtacg ggcgacaaac agctggatta tttgaaatat tacaacttca agggagttgt
    18661 gaattttgaa ttggctctac agcaattggg atacagttgc ggagatgtaa ccgatgtaat
    18721 cttgactcat ttgcatttcg accattgcgg aggatgtact tattatgatt cagatcggaa
    18781 aaatgtattg ctcaccttcc caaatgccgt tcactgggtg ggagaaaggc aatggataaa
    18841 ttttctggag cccaatgtga gggaaggaga ctcttatttt cctgaaaata tgctgcctgt
    18901 gcagcaagca gggaaattga aactggtcaa ggaaaacagt tttctctgtc ccgaaattga
    18961 acttcgtatt ttcaacggac atacagtagg gcaattggtc agttatattc aatatttggg
    19021 gaaaacatgg atttatgtgg gtgatgtaat tcctgtagca ccatccattc cattggcttg
    19081 gatatcggct ttcgatgctt atccggtaac ttcgttggaa gaaaagaaga ttcttttaca
    19141 ggaagctgaa gaaaaacatc aaatcctcct attcgagcat gatgcttata ctgaatgttg
    19201 tactgtgaaa aaatcgaacg ataatttcag aatggatgaa agttttccgt ttacaaaccc
    19261 agtgaaatag tctcttttta gtactataga cagcactata tagaaaatca gactgccata
    19321 gtagtgatat gtttttaaaa aattttacgg taaagcatta tcgctttgtc ttctgtccga
    19381 cattcaaccg gacagaagac aaagcgataa acttgttaaa agaatcattg agcttacttt
    19441 aattgtcgct tgactctcta tttacagaat ttctgtcgtc tcccaactga ttataaatcg
    19501 gttataggag aatatttggg aaccaaagct ttcataacta tccaaccaat gagataggca
    19561 acagcacaga aagaaaaaac gataaagtaa cccgcttctt cgcccttaaa aatcaaaaat
    19621 gacatttgtg tttgccccgc aaagtcaaac aaagcacctg aagccttatt aattaacatt
    19681 gaacctatac ctcctgccat tccgcctatg ccggtaactg tagccacagc gcttttaggg
    19741 aacatatcgc ttacggtaga aaaaatatta gccgaccatg attgatgtgc agctcccgct
    19801 atacctatga taataatggg caaccaataa gaaatatgac ccaatggctg ggcaaaaagg
    19861 gccaacaatg ggaagaaagc aaaaatcaac atggctttca ttcttccagc atagggatgc
    19921 attccttttt tatcgacaaa ataggtcggg agccagcctc caataatgga caaaagtgta
    19981 attgcataaa gaacaaatat agccagctga cttcttggat cggatgattt cagtccgtac
    20041 acagaactca aatatgcagg agtccagaaa agatagaacc accaaacccc gtctgtcatg
    20101 aattttccga aagcaaaagc ccatgtctgc ttaaatttaa agcactgttt gaaactcagt
    20161 ttcttttcct tatccgtatt ggcattagga ttcacagctg cttcatccgc atgatccaaa
    20221 tgaatgtact ccaattcgag tttattcact tttggatgca cttctggttt tttgtaaata
    20281 aatgcccaaa accccatcca aataaaaccc aaagctccga taatgataaa cgacatttcc
    20341 caaccccact tttctgcaat cacaggaatg acaaccggtg cagccaatgc tccaaccgta
    20401 gaacctgcat taaagatact ggtggcaaaa gcacggtctt ttttcgggaa atattctgct
    20461 acagccttga tagaagccgg aaagttaccc gattctccga tggcaagtac caaacgcgca
    20521 aaaatgaata aagttacact cacattcagc accatcgaaa cattattcac tgtagcaata
    20581 gcttcctttg ctccctcaaa accaaccaac catttacctg caacgattcc tgaagtggct
    20641 attccacaaa aagcatgtaa cacagctcct aatgaccata ttccaattgc ccacagaaaa
    20701 cctttttttg tatccaactt gtcaataaat cggcctgcaa acaacatccc aatggcataa
    20761 aataacgaaa ataaagaagt aatggtacca taatgagtat tggtccaatg aaattcagga
    20821 gctatgaaat ccttccacgt cagcgaaaga acttgcctgt ctaaataatt gatcgccgta
    20881 gcaaaaaaca atagtgagac gattgaccac ctgaaatttg acattttttt agcttgctgt
    20941 tccatttcaa ttgttttacg atattagaag ttttacaatg tttgccttat gaagcaaata
    21001 attatttgat gtttttttga atttttttta tcaagtctaa tgtctccctg caaagttcag
    21061 ttatttttcc ccaatcatgg gcagcaatca catccgcagg aaacaaattg gaacccatcc
    21121 ccacgcacat gacaccggca tcaaaccagc ttttcaaatt tgcctcttcg ggttttactc
    21181 cgcctgtaac cattaaattc gaccagggca tgggcgcttt caatgccttt acaaaggccg
    21241 ggcccaagac ttcgccggga aaaaccttgc aaacatcaca accggcttcc tgagcaaatc
    21301 caatttccga aaccgatcca caaccgggca tatagggcac caaacgccga ttgcaaattt
    21361 tagcaacatc gggattgaac aaaggaccta ccacaaaatt ggctcccaac tgaatataaa
    21421 gtgctgcggt gggagcatcg atcgccgaac ctacgcccat aatcatgtca ggacagtttt
    21481 tttgaacata tttaaccact tcgccgaaaa catcgtgcgc aaattctcct ctgttggtaa
    21541 attcaaatgc tctgattcca ccctcgtaac aagctttaac cacgcttttg gcaatttcgg
    21601 gattactatg gtaaaacacc ggcaccactc cggtgaattt tatggaagaa agtacttcca
    21661 atttattgaa cttcatgatt ttattaggta ttttaatatt ctgattgtaa aatttttaaa
    21721 atgttaccgg gaaactcttc cgctttcatc tccgctcatc aaagcttcca cttccttgat
    21781 ggtaaccagg ttataatcgc cgtaaatggt atgtttcagg caggaggcag ccacagcaaa
    21841 atccagcgcc ttctgatcgt tgccttcgta agtcaacaat ccgtaaatca gtccgcccat
    21901 gaaagaatcg ccgccaccca ctctgtccac aatatgcgtt atgtcgtaac gtttcgattg
    21961 atagagtttt tcaccactat agagcacgcc accccatgta ttgtgattgg cattaatgga
    22021 tccgcgcaag gtaatgatca ctttttgggc acgtggaaat tttttcatta attgcttgca
    22081 aaccgattca aattcggcag aatcaatttc tccgccggta cgggatacat cgaaaccttc
    22141 cggcttgata ccaaaaactt tttctgcatc ttcctcattt ccgagaataa tatcgcaacc
    22201 ttcaaccagt tcgggcatta tttcggaagc ggattttccg tatttccaaa gattttttct
    22261 ataattcaaa tccgtagata ccgttacgcc caattcattg gccactttta cggcttcgag
    22321 acaaacgtcg gcagttccct gcgaaatggc cggcgtaatg cctgtccagt gaaaccaatc
    22381 ggcatctttc aatacttctc gccaatcaat catgcccggc tcaatttccg caatggacga
    22441 atgtgcccga tcataaatta ccttgcttcc tcgtgctacc gctcccgttt ccaaaaaata
    22501 aattcccatg cgttcgccgc cacgtaaaat gtaatgcgtg ccgacattgt gcttgcgcaa
    22561 gtccatgatg cacgattcgg caatttcgtt ttgtggcagc cgcgtaacaa attcggtctc
    22621 cattccgtaa ttggccaacg aaaccgccac attggcttct cctccgccaa aagtggcact
    22681 aaactccgta gcctgactaa atctttcata tcccggcgtg gccaaccgta gcattacttc
    22741 tccaaatgtt acaatttttt tgctcatttt tcagtctcct ttttaattgt tgataaataa
    22801 ttggtcgacc aaaattactg cttttatatg cactttcaaa atttttttat ttcgttttca
    22861 aaaaaaattt ttttccttga aaatctgcgg aaaaaataaa aagaagacga aatttttgaa
    22921 attagtttta ttaactttgc tcaattaatt ataagataaa aatccggcga aaaacaagaa
    22981 caagtgctaa aatttcttat attgccataa agtaaaaagc aaatcatgaa ttgaaaatca
    23041 ggtttcaatg aaaattctgt gacttgttgg caaaagtaca aaacaatttc atattatcaa
    23101 atatttattt taattttgga catatttttt ttgtgcacgt acacaaaata acaattaaaa
    23161 agcaagatat ggaaaaagcg aaaggcaaaa tacgcattaa ggatattgcc aggatggcag
    23221 gagtatctga aggtacagtt gacagggtgt tgcatcagcg aggtgtagtt tccgaaaaaa
    23281 gtcgaaaagc agtaaagaaa gtattggaag aaattgatta tacaccaaat ttgcttgctc
    23341 ggtcgttggc ttcgaaaaaa aaatatcgtt ttatttgtct tttccctgcc tataacaaag
    23401 gtgaatactg ggaaagtata gaaaagggat ttcgacaagc ctctgaagaa tttaacaatt
    23461 acaaagtaga ggtaatgatt gaatattttg atcagtttga tagcagctca tttttagcaa
    23521 cggcaaaagc tgtttttgtc tataatcctg atgccattat tatggctcca gttttcaggc
    23581 aagaaaccat tttatttgcc agagagctta cagaaaaaaa gatccctttt tcttttattg
    23641 attctatggt agaaaatgtt gaatttacat cttattatgg tcagcattct ttgcaaagcg
    23701 gatatattgc aggcaaatta ctgtttaatt ctttaccgaa agaaagcaaa attttagtac
    23761 ttcataccta cagaaaaggt ggttctgggt ctattcagac ggaaaataga aaaaaaggat
    23821 ttttgcaata tattgaagaa aataatttga aaaatttctg tgaaatattg tctatagaaa
    23881 ttattgaggg aaatgatgaa gaaaacatgc gcttaatccg gaatgctttt atccaacacc
    23941 cggatattcg ggcagccata acttttaact ctaaagttca tagattggcc aaatatttca
    24001 aaaacctttc tcattcggac attaaattaa ttggatatga tgtattggag gaaaatgtaa
    24061 aatgcatgaa agaagggttc gtttcttttt tgattgctca acgacccgaa aaacaggctt
    24121 attgcgcagt aagagacatg agttttcact tgattttcga taaagaagtt aataaaatca
    24181 attatgtgcc tatcgacatt ttgatgaagg aaaatattga ttattatttg aatttcaatg
    24241 aatgatcgaa ttaagcccat gaggattaat caagagaatc aagtagcgtt tttgcagtgc
    24301 aaatttttaa tctacgttgc caaaaccaac aaaaatattg ttttgggaaa aagaagtttg
    24361 gatatttttg aagaaataat ttcctttaaa aaaatcattt ttaagaagat ttcatcatga
    24421 atacttctat tgcttcagat tggattcaac ctgccctgta tcagcaacca ttggaaaacg
    24481 gaatggtaaa atgcgttctc tgtccccatc aatgtatggt gcacgaagga aaaaccggac
    24541 tttgtcatac ccggcaaaac atcaacgggg ttctatttac gcttgcttac gctaatccgt
    24601 gttcactgaa tgtcgatcca atcgaaaaaa aaccactgtt tcatttcctc cccggctcaa
    24661 aaattttttc attggctacc gccgggtgca atttcaaatg cttaaactgt cagaattggg
    24721 aaatttctca aagctcacca caagaagtga ttcattacga tttgttgccg gaagcagttg
    24781 tggaacttgc ctcgaatcga cagattgcca gcattgcctt cacttatacc gaaccgactg
    24841 tattttatga atatgtttat gacacggcga agttggcaca tcaaaaaaac atgaaaacgg
    24901 tgattgtttc caatggctat attaacgaaa aacctttgct ggacttgtgt ccatttttgg
    24961 atgccgccaa tattgacttg aaatgttttg acaaccattt gtaccgaaaa ctcaccggcg
    25021 gaagtctgga gcctgtttta aaaaccttga aaatactgaa agaacaaggt gtttggctgg
    25081 aaattaccaa tctgattgtt cccacttatt ctgatgcccc agaaatgatt gagaatatgt
    25141 gcaaatggct ggttgacaat ggatttgaag atataccgct ccatttcagc cgatttttcc
    25201 cggccaatca attacccgat ttacctccaa cacccataaa aacattgctt gcagcccgag
    25261 aaattgccca aactgccgga atgaaatacg tttatgtggg aaatgttcct gcacttggtt
    25321 atgaaaatac tttctgtcct tcctgtaaaa aattgctgat agaacg
//
LOCUS       DAOSIB010000023        24781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_2245_length_24781_cov_12.477958, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000023 DAOSIB010000000
VERSION     DAOSIB010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 24781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24781
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2245_length_24781_cov_12.477958"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..416
                     /gene="ffs"
                     /locus_tag="PLD44_03580"
     ncRNA           317..416
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLD44_03580"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(452..1780)
                     /locus_tag="PLD44_03585"
     CDS             complement(452..1780)
                     /locus_tag="PLD44_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOF98454.1"
                     /translation="MASPINFFVRIYNFFVSDIWKIAENELTRTRRFFYHLIKIILYA
                     IRGYSTDKLAIKASALTYSILFAVVPVIAIIIAIGRGFGMETLIEDALKNTFIAQANL
                     VPVVMDFVKKYLESIQGGVFLGVGIVILLSAVVNFFMQVEDTFNSIWQVKKSRSFLRQ
                     FTTYFSIILILPLFIVLSSGFSIFVNTTISQIPGFQILNPLLKVGANLLPYLINWLIF
                     TFMYLFIPNTQVRFINALIAGIIAGTAFQLFQILYITGQINLSRYNAVYGGFAAIPLL
                     ILWIRISCLIVLLGAELTYASQNIRNYESELDTQNISIRNRKLISLYITYLVIKQFEL
                     QKPPLSSEQIASDFKLPIRLVNQILNQLVNISVLIEVFNDKNKSKSYQPAMDINQLTV
                     GLLFEKLESYGTGITLLKNNEKLKNFWEKTSAVLAAGSKESSNEILIKDL"
     gene            complement(1865..2222)
                     /gene="rnpB"
                     /locus_tag="PLD44_03590"
     ncRNA           complement(1865..2222)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLD44_03590"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2300..4126)
                     /locus_tag="PLD44_03595"
     CDS             complement(2300..4126)
                     /locus_tag="PLD44_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOF98455.1"
                     /translation="MEYQLTDFLPVTRKELELRAWEDVDVVFFTGDAYVDHPAFANAL
                     IGRLMESLGLRVAIVPQPNWRDDLRDFKKMGRPRMFFAVSAGNMDSMVNHYTANKRLR
                     SNDAYSPDGKPGFRPDYATVVYCNILKKLYPDVPILIGGIEASLRRLTHYDYWSDQLK
                     QSILCDAQADLLIYGMGELPLTELIRQLKNGKQFHELTDIPQTVYLSDNIPQDNLWNT
                     IELPSFETCLHDRKKYASAFKTIEEQSNRYQADRLIQKTEDKWVVVNPPYPPMIDAQL
                     DASYELPYTRMPHPKYKGKTIPAYEMIKFSVNIHRGCFGGCSFCTISAHQGKFIVSRS
                     KKSILNEVEKIKLMPDFKGYLSDLGGPSANMYQMKGKNIEICKKCKKPSCIFPKVCFN
                     LNTDHHKLLDIYRSVDALQGIKKSFIGSGIRYDLLLHESADELTKKSQSEYIRELILH
                     HVSGRLKVAPEHTSDRVLAIMRKPSFQYFIRFKTQFDRINKEANLNQQIIPYFISSHP
                     GCTKEDMAELAVITKNLDFKLEQIQDFTPTPMTLATEIYYSGFHPYSLQPVYVPKTKE
                     EKLEQRKFFFWYKPEYRSQIKRDLLMMQRPDLLKKLFQNERK"
     gene            4443..4757
                     /gene="rplU"
                     /locus_tag="PLD44_03600"
     CDS             4443..4757
                     /gene="rplU"
                     /locus_tag="PLD44_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOF98456.1"
                     /translation="MYAIVEILGQQFKVQAGKKLYVHRMEEAEIGSQVEFDKVLLVDN
                     DGSIAVGAPCVEGAKVVCDVVGHVRGEKVIVFHKKRRKGYRKRTGHRQDFTELLIKEI
                     VA"
     gene            4777..5037
                     /gene="rpmA"
                     /locus_tag="PLD44_03605"
     CDS             4777..5037
                     /gene="rpmA"
                     /locus_tag="PLD44_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOF98457.1"
                     /translation="MAHKKGVGSSKNGRESESKRLGVKIYGGQFAKAGNIIVRQRGTV
                     HHPGKNIGMGKDHTLYALVDGKVEFRKKVGNRSYVSINPEMN"
     gene            5213..6484
                     /gene="serS"
                     /locus_tag="PLD44_03610"
     CDS             5213..6484
                     /gene="serS"
                     /locus_tag="PLD44_03610"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOF98458.1"
                     /translation="MLTLKYIAENSEDVLKRLKKKHFDGKELISKVLELNAERKKTQY
                     DADSIAAEINKISKEIGILYKQGKTSDAETLKQKTLELKENIKLLEEKQNQIETQLNE
                     ILVQIPNLPHESVPEGKLAEDNQVERTGGKIPSLPTDALPHWDLAKKYDIIDFETGVK
                     ITGAGFPLYKGKCARLQRALINFFLDNAREAGYMEVQPPYVVNEASAYATGQLPDKEG
                     QMYFCQEDNLYLIPTAEVPVTNIYRGMILNENELPIKNVAYSACFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQIQHPEKSYETFYEMVHYVEGLVEKLELPWRTLRLCGGDMSFTSA
                     LTFDIEVYSAAQQRWLEVSSVSNFESYQANRLKCRYRSADKKIELCHTLNGSALALPR
                     IVAALLENNQTPEGIRIPKALIPYTGFEIID"
     gene            6690..7598
                     /locus_tag="PLD44_03615"
     CDS             6690..7598
                     /locus_tag="PLD44_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOF98459.1"
                     /translation="MESISSFAELIAHLKFLNNRKRVAVANAVDPHTLEAVLRASDNG
                     FVEAFLIGDLAMIESPHFFEHKLTPYVHIIDIPEVEEATREAVRMVRTGEADILMKGL
                     VNTDVILRAILNKENGLVEKGKIITYNAALEIPDYQKLLFFTDPAVIPSPTVEQRTAM
                     IKYVTETAYKFGITRPKIALIHATEKANPKIQYMQDYLDIMEKWRMGEFGDVIMDGPL
                     DIFLALDPERGSIKKVSSPIHGDADILIFPSFECANAFYKGLMVFAKAEMAGLLQGTQ
                     KPVVLTSRSESVNSKYYSLAMACVMG"
     gene            complement(7588..8742)
                     /gene="lpxB"
                     /locus_tag="PLD44_03620"
     CDS             complement(7588..8742)
                     /gene="lpxB"
                     /locus_tag="PLD44_03620"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOF98460.1"
                     /translation="MKYFLIAGEASGDLHASHLMHELKKQDLQAEFCFLGGDLMWTEG
                     GEMIRHYRQMAFMGFIAVLKNLSVISVNIKDGKQALLKFQPDVLILVDYPSFNLRMAE
                     FAKKKLNIPVYYYISPKIWAWKEFRIKKIRRYVDRMFTIFPFETEFYAKHRYPVEYVG
                     NPTVDAVEEFKKAYRGNFQTFRQSNNLVDKPVIALLAGSRRQEIKSCLPRMVEAAMQF
                     PDYQPVVAGAPGVEKDFYFSVLKDTSVAVVFGQTYELLFHAEVAIVNSGTATLETALI
                     GTPQVVVYHVAFGKVAYWLKKLFIKVKYISLVNLIAGKEVVMELYAHLFTVENVKNEL
                     NRILTDAHYRQNMKNEYKKVSEILGEPGAASRAAKKIFELLHSDKSVLTP"
     gene            complement(8792..9100)
                     /locus_tag="PLD44_03625"
     CDS             complement(8792..9100)
                     /locus_tag="PLD44_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98461.1"
                     /translation="MEFVNSYAVKRLNHFYIGFLVGLILPCVFVWLYITSFYPVDISF
                     FEILKRLYPGVLLGKLLLLSIVPDLLMAFVFYKNDAFRLTSGTIVGGLPFLIASLFML
                     "
     gene            complement(9088..9846)
                     /gene="surE"
                     /locus_tag="PLD44_03630"
     CDS             complement(9088..9846)
                     /gene="surE"
                     /locus_tag="PLD44_03630"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOF98462.1"
                     /translation="MNKNLILISNDDGIDAKGIKILTKLMMQLGDVVVVAPDIPRSAQ
                     SSALTVTQPIRYRKIEEEEGMVRYSCTGTPTDCVKIALNEILNRKPDLLVSGINHGSN
                     SAINVIYSGTMGAVFEGCENGIPSIGFSLASHFPDPDFSFFEPHVLKIAQIVMEKGLP
                     ESVCLNVNAPCGEIHGVRVARQCKGYWTKEFAARTDPAGRAYYWLTGYFENMEPDAED
                     TDEWAISHGFISIVPDKIDLTAYDAMDLIREWNL"
     gene            complement(9921..10652)
                     /gene="gldF"
                     /locus_tag="PLD44_03635"
     CDS             complement(9921..10652)
                     /gene="gldF"
                     /locus_tag="PLD44_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HOF98463.1"
                     /translation="MISIFKKELGSFFSSATGYLVIGIFLALTGLFLWVIPGEFNIIE
                     SGYANVDGLFYLAPWLFLFLCPAITMRSFAEEKKSGTWEILITKPLSITELVLGKFLA
                     GWVLVLIALFPTVLYYFSVYFLAEPVGNIDAGAFWGSFIGLIFLSGVYIAIGIFSSSL
                     SNNQIISFVVSVLLCFIFFYGFDLLGSFFSSGAVIDFVQNLGINAHYRSMSRGVLDMR
                     DVGYFLFLGGLFLGIIVWKNSKSDK"
     gene            10813..11985
                     /locus_tag="PLD44_03640"
     CDS             10813..11985
                     /locus_tag="PLD44_03640"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOF98464.1"
                     /translation="MFFAIYSFGFAQTFKNYSLSADFIYGEILKHNKHLENLVKGPLK
                     GEEFSIEWQTMGEKAWHQYLNFPSVGISTAFLDFSHPDTLGYAVALYPYLKFPIVRQQ
                     HFNMAFKAGGGLSYVTKTFYNATAYHPDGSVYLNKSNAAIGSHVNVYLTANLNMEIPL
                     TKGWSITSNWGWNHTSNGSIVQPNSGINMLDMYLGIKYFPNYERYIPPKPQPLPDLPH
                     KIEIEMAFTGGIRQLYYKDDKTYPIGTFNIAAYRTMTNFYRMGWGADLFYDGVFNGKS
                     AFKRTYLTTDELKYKLRIGASFRHELLVGRLTTGFHFGLYLYNPVKNLEPYEEAKNGT
                     LNKPLIYKYNIEKEDGWFYTKAVLKYALDEHFLLSLGLKTHLQKAEFIDAGIGYKF"
     gene            complement(12004..14217)
                     /locus_tag="PLD44_03645"
     CDS             complement(12004..14217)
                     /locus_tag="PLD44_03645"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOF98465.1"
                     /translation="MNATQIDTTYKAILRFVSASRLKPAFDRIKIMIDDLQYGEFSDR
                     FNELERNYKFLLEYYLKGVQDPERKSVYSKLVAKLLILLADVREELMMKNSSNFEYSQ
                     KRFFPYTRKHQTVNELFNALQYFHKQSALLSENKEQYKIELSRMRSNFEDALDELFGV
                     FWLATRYSEEENNLYKKLLEADDKYWVEKSLMVSAVTLNLWRKFDEQKIMLLFDSCEN
                     DDQKVVQRALVGLTFILAKYNIYLPYFPSIRNRLVLLVDQNRIAENFQNIIIQIIGTT
                     ETEKISRKLREEILPEMMKINPLLRDKMDAENFLNSDEWAEANPEWQEIFDESGLSDK
                     LKELSDLQLEGADVYMSTFSMLKNFTFFSQFSHWFLPFDTENSAVNQLFESDEKTILS
                     AFVNSNVMCNSDKYSFCLSVLQMPESQRNMLKHSFKMESDQMDEMEEEMSILSPETIT
                     KNISKQYIQDLFRFFKLQPHKDDFEDMFSYSLTLHRTWLFDILSSIPGFSESVAEYYF
                     SKGYYLQALELYEKIQSESQPSAALYQKMGYAYQQTSQLQKALDAYLKADMIQPDDGW
                     TIRKIALCYRLLGNYEKALAFYQHADFLKPGQYSTQIHIAQCYFELGKFKDALAIYYK
                     LDAENVDDIKVWRAIAKCNFFDGNLDKADYYSEKIILAEPNAYDYLLSGHISWCRRQL
                     SAAADAYRKSLEMLQNKWEVFQDLFNEDKDRLISFGIREEELPLMMDALLYFENE"
     gene            complement(14293..14994)
                     /locus_tag="PLD44_03650"
     CDS             complement(14293..14994)
                     /locus_tag="PLD44_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOF98466.1"
                     /translation="MTKKPAYELKPIEEVFMVWDVLTPDERQFVRNNYTVHHFKKNEL
                     IQSEGEISTHMMILAAGKAKVYKDGIGSRTQIIRMLKPGENFGYRAIIANEPNSTNVA
                     AFEASTVYMLKADLFLSILRHNIAVCYRFLEELASDLAVSDARTVNLTQKHIRGRLAE
                     ALLFLKKRYGVEEDGATVNIYLSREDLANLSNMTTSNAIRTLSNFTNEHIIAVDGRKI
                     KIIDEDKLRKISKMG"
     gene            15271..16518
                     /locus_tag="PLD44_03655"
     CDS             15271..16518
                     /locus_tag="PLD44_03655"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HOF98467.1"
                     /translation="MKRAIFVSLLSFILFPIFSQNLTFSDSAKVSLLTCSPGKKVYEK
                     FGHTAIRVNDPITGYDVVFNFGVFDFNTKNFYLKFIKGDTDYQLAVYDTKSFLEEYKS
                     RNSMVWEQKLNLTLNEKRNLLHSLIKNYEPENRFYRYNFVYDNCSTRPRDKIVQSVNG
                     KVEYHLYNEPVTFREMIAQYTGVNSWMQFGLDLIFGRDADKSVSRNVSMFLPENLMYE
                     FQAAHIIAADGAKRHLVAENKILIPKQEEKYSKPLPIFQPVFVCTFLLILGIIITIFN
                     NKKSFRLFDSLLYIVSGLGGIIVFYLAFFSSHPLVKNNFNLLWLNPLNFALGILLWFH
                     KMKPYVFYYQVLNCLLIFLALVAYAAGLQVFHPASFPLILLLLLRTVRWTATTKKDFS
                     LKKKNNRDYYTNSNGLHHHSRKS"
     gene            16551..>16717
                     /locus_tag="PLD44_03660"
     CDS             16551..>16717
                     /locus_tag="PLD44_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOF98468.1"
                     /translation="MTLHSNKNFRFVGIIPARFASTRFPGKPLADIGGKPMIQRVYEQ
                     ARKVLQTVYVAT"
     assembly_gap    16718..16817
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16818..17174
                     /locus_tag="PLD44_03665"
     CDS             <16818..17174
                     /locus_tag="PLD44_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOF98469.1"
                     /translation="EEYDYILNPNRPKIVLNKNNEALYFSRQVIPYQRNTANEHWISN
                     HQYFVHVGIYAYRTEILKELCSLPVSSLEKAESLEQLRWLENGYRIKVGFTTAESYGI
                     DTPEDLEKVRKMFLGK"
     gene            complement(17543..18574)
                     /gene="mltG"
                     /locus_tag="PLD44_03670"
     CDS             complement(17543..18574)
                     /gene="mltG"
                     /locus_tag="PLD44_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOF98470.1"
                     /translation="MKKKVRKILFAALAVPLIVVVYLTYVFFAPNIFPKKETETFVCI
                     PENTSFEDVVKLIKNESRVWDRYSLRLTAKIFDYDKNIRSGRYALKKGMNNFALIRNL
                     RSGRQVPLRLTFNNIRTKEQLAGRLSSQLMTDSLTFLKLLNNSQFLSQYALNPDNSVV
                     IFIPDTYEVFWNIKPKKLFDRMYREYLKFWNEERRQKAASIPLTPIEVSILASIVEEE
                     SNKSYEQPIIAGVYINRLKNKMPLQADPTIKFAWKDFSLKRIKGQHLQINSPFNTYKF
                     QGLPPGPIRLPSPQVIDAVLNYSHHSYLYMCAKETLNGEHNFAATLAQHLINARKYQR
                     ALNERQIFE"
     gene            18779..18865
                     /locus_tag="PLD44_03675"
     tRNA            18779..18865
                     /locus_tag="PLD44_03675"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18813..18815,aa:Ser,seq:gct)
     gene            18974..20341
                     /locus_tag="PLD44_03680"
     CDS             18974..20341
                     /locus_tag="PLD44_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOF98471.1"
                     /translation="MEKTKKFILTEKELPTHWYNIVAEMQNKPMPILNPATKQPMKEE
                     ELYPLFAEELSHQELNQTDAWIEIPEEVREKYKIYRPTPLVRAYDLEKALDTPAHIYF
                     KNESVSPVGSHKLNTALPQAYYCKQEGVTHLTTETGAGQWGTALAFAAKTFGLELFVY
                     MVKISYEQKPYRRSLMQTWGAQVIASPSMSTNAGRKILSEHPNYLGSLGTAISEAIEL
                     AMNTPNCKYTLGSVLNHVSLHQTVIGLEAEKQMEMAGEYPDIVIGCFGGGSNFSGISF
                     PFLRHNIKDGKKTRFIAAEPASCPKLTRGVFEYDFGDEVGYTPLLPMFTLGHNFAPPH
                     IHAGGLRYHGAGTIVSQLKKDNLVEAVDIDQLDSFKAGVLFAQTEGIVPAPESAHAIA
                     VAIHEALKAKEEGTPKTILFNLSGHGMVDMTAYDQYFAGDLTNYSLSENDIAENIKNL
                     EHLAK"
     gene            complement(20423..21679)
                     /locus_tag="PLD44_03685"
     CDS             complement(20423..21679)
                     /locus_tag="PLD44_03685"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOF98472.1"
                     /translation="MIVYKFGGASVRSAAGIKNLASIVSGVNDNLFIVVSAMGKTTNA
                     LEGVLEDFMQADHASALAKFQQIENYHNEILAELFPDSANPVAILQPLYDEITSMLKL
                     SVGDDYDRWYDKIVSYGEIISTQIVSKFLNEAGIQNGWLDMRKLLITDSNFREANVKM
                     KESQQKLTEAVNFENYPVYIGQGFIGANTKGDPTTLGREGSDYTAAVVANLLNAENLT
                     IWKDVPGILNADPRLYPQAKLVPELTYSEAVELAYSGAQVIHPKTIKPLQNKNIPLYV
                     RPFGNPSEKGSVIKAHVEKPAGIPFYSLLKNQVLVTIRPIDFSFVLEENLPKVFSMIN
                     KHRLKVSMIESSAISISVAIGNSRYLPAALDELSNEFDVSYNENLELLTVRGISDEIA
                     QEVTNHREVLLMQRTRRIGRFLMKER"
     gene            complement(21838..23193)
                     /locus_tag="PLD44_03690"
     CDS             complement(21838..23193)
                     /locus_tag="PLD44_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOF98473.1"
                     /translation="MCFITENLNASNKKTTVFVIELKKDIGGTAWVYCQKAFNQAKEK
                     NADVILMHMNTYGGEVVFADSIRTRILNSPIPVYVFIDNNAASAGALIAIACDKIFMR
                     PGAAIGAATVVNTTGQQMPDKYQSYMRATMRTTAEAHGKDTIIQGNDTIFQWKRDPKI
                     AEAMVDDKVSIPGIIEAGKTLTFTAEEALQHGYCDGIAESIDEIIKNHITEANFEIIV
                     FQPSFWDKVKGFFSSSIVRSILIMLIIGGIYFELQTPGIGFPLAISILAAVLFFVPLY
                     IDGLAANWEILIFLLGLVLLALEIFVIPGFGIAGISGILFSVAGLTLSLINNVNFNFD
                     GVQAGTVGKSLLTVLVGITLGFVLILYLSNKIGSTGIFKKMALNTALENKEGYIGVSM
                     DELQLVGKTGYASTVLRPSGKVNVEGKVYDAVSTEGFIEKGVQVRVLEYRTGQIYVEK
                     I"
     gene            23476..23982
                     /locus_tag="PLD44_03695"
     CDS             23476..23982
                     /locus_tag="PLD44_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOF98474.1"
                     /translation="MKPNQFENELIALQNNMRNFAFSLTLNREDAEDLLQDTTLKVLD
                     NREKFTKNVNFKGWVLTIMKNIFINNYRKIVRNQTVIDKTEDMYHLNLPQNSGFDSPE
                     GSYSIGEINSNIEAFPDEYRIPFSMHLQGYKYEEIAETMHLPIGTVKSRIFLARKRLQ
                     QQLKDYRD"
     gene            24103..24471
                     /locus_tag="PLD44_03700"
     CDS             24103..24471
                     /locus_tag="PLD44_03700"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOF98475.1"
                     /translation="MNTAIVILGSNVEADQNIDAAKERLSEYFEIDLCSSRIGSDAVG
                     ENYVAPFLNEAVKLYSDETAEETIAIFKQIETEMGRTPESKITGLVPIDIDLIFWNDN
                     LMRNDYNRFSFVKRCVDEIK"
BASE COUNT         8139 a         4905 c         4393 g         7244 t
ORIGIN      
        1 tgtattctat gttactgctc aaaaattggt gttatttctc cgtaattatt tccatatact
       61 cttaaaacta tacctccatg ctgccgcaaa actttcgtgt ggtactgcgg tagtacatga
      121 aactgatttt gtcccggagg gacaaccttt tggtagaaaa aggcaatgaa caaaaaaatc
      181 ccgtagggat gaccagagga atgatgttga tttaaagttt tgccacacga gcaaattttt
      241 tttgtcatgt gctgtatgct gaggaaaaaa tacagagata tttggaaata tgataaaaaa
      301 tcgtaccttt gcaacggggt aagtcctaca cgaccagctc ctttcaaact cccccagggc
      361 ttgaccgcag caagggtaga aagttgtagc ggtgcgatgt agggagcttg cccttttttg
      421 tttcaataca ttaatgttgt aaacaaataa tttacaaatc cttaattaaa atctcattag
      481 aagattcttt actacctgca gctaaaacag cggacgtctt ttcccagaaa tttttaagtt
      541 tttcgttatt ctttaaaaga gttattcccg ttccgtaaga ttccaacttt tcaaacaaca
      601 atcctactgt taattgattg atatccatcg caggctggta acttttagat ttatttttat
      661 cgttgaaaac ttctattaat accgatatat tgactaattg attgagaatc tgattcacca
      721 aacgaattgg taatttaaaa tcggaagcta tctgctcgga agataagggt ggtttttgca
      781 attcaaattg tttgataaca agataggtaa tgtataaaga aataagcttt ctgtttctta
      841 tgctgatatt ttgagtatcc aattctgatt cataattacg aatgttttga gaagcataag
      901 tcaattctgc tcccaaaaga acgataaggc aagaaatgcg aatccaaaga atgagcaggg
      961 gaatcgcagc aaaaccgcca taaacagcat tgtaacgcga caaattgatt tgtccggtga
     1021 tatacagaat ttgaaatagc tgaaaagctg tccccgcaat aattccggca atcaaagcat
     1081 tgataaatcg tacttgggta ttggggataa ataagtacat aaaagtaaaa atcaaccaat
     1141 tgattaagta gggtaataaa tttgctccaa cttttaaaag cggattcaaa atctgaaaac
     1201 cgggaatttg agaaatggta gtgtttacaa aaattgaaaa tccgctggaa agcactataa
     1261 aaagcggtaa aataagaata atggaaaaat aggttgtaaa ctgacgaaga aaggaacgcg
     1321 atttctttac ctgccaaatg ctgttgaaag tatcttccac ctgcatgaaa aaattcacta
     1381 ccgccgaaag caaaattaca atacccacgc ccagaaatac gccgccctga atgctttcca
     1441 agtatttttt aacaaagtcc ataactaccg gcacaagatt cgcctgcgct ataaaagtgt
     1501 tttttaacgc atcttcaatc agtgtttcca ttccaaagcc acgtcctatt gcaatgataa
     1561 tggcaattac aggtaccact gcaaaaagga tggaataagt cagagcagaa gcttttatgg
     1621 ctaatttatc agtggaataa ccacgaatag cataaagaat tatttttatc aaatgataaa
     1681 aaaaacggcg ggtacgcgtg agttcgtttt cggcaatttt ccaaatgtcg gaaacaaaaa
     1741 aattgtaaat gcggacaaaa aaatttatcg gagaggccat aagcagatga atgatgattt
     1801 acaattttgt gaatttgaga tttaccacaa aaaatatcat tgaaaaatca gccacttttt
     1861 taaaaaaaat agcaggtttg taagccgggt tttgtacttt gcaaaagcaa agtgtttgtc
     1921 atttatctga agcaaatgtc gccacttgct ttaagcgatc tacccctcga catcggacga
     1981 gccatcctac atgcgtcgat atacatgatc ttgcaaccca taaggcgtac ggctaaaagt
     2041 gttgccactc ttaccggtga gctcttgcct caccttttca cccttacctc aaacaaggcg
     2101 gttgttttct gttacgctac tctgcccttg cggacagctt cccgttagga agtatggcgc
     2161 tctgtgttgc ccggactttc ctctttccaa aaaatttgga aagcgacaaa ccaacctgct
     2221 atcaacttac aaagttactg atttttatac acttttcaaa aaaaacggga acaaaaaaat
     2281 aactgttata tacgataaat tattttcttt cattttgaaa caatttcttg agcaaatcgg
     2341 gacgttgcat catcaataaa tcgcgtttta tctgactacg atattcaggt ttgtaccaaa
     2401 agaaaaattt tcgctgctcg agtttctctt ctttggtttt gggaacataa accggctgca
     2461 aagaataggg atgaaatccg gaataataaa tttcggtagc cagcgtcata ggtgtcggag
     2521 taaaatcctg aatctgttcc agtttaaaat ccaaattttt cgttataacg gccagttcgg
     2581 ccatatcttc ctttgtacaa ccgggatggc tggaaataaa atacggaata atttgctgat
     2641 ttaagtttgc ttccttgttg attctatcga actgggtctt gaaccgaatg aaatattgaa
     2701 aagaaggctt tctcattatt gccaacacac ggtccgaagt atgctcagga gccactttca
     2761 ggcgacctga aacatggtga agaatcagtt ctcggatgta ttcggattga ctttttttag
     2821 tcaactcatc agccgattcg tgcaaaagta aatcgtaacg gataccgctc ccaatgaaag
     2881 attttttaat tccttgcaag gcatcaaccg aacgataaat atccagcaat ttatgatgat
     2941 cggtgttgag gttgaaacaa actttaggaa aaatacagga aggtttttta cattttttgc
     3001 aaatttctat gtttttccct ttcatttgat acatattggc cgaaggtccg cccaaatcac
     3061 ttaaataacc tttaaaatca ggcataagct taattttctc tacttcattc aaaatagatt
     3121 tttttgacct tgacacaata aattttcctt gatgggcaga gatggtacaa aaagaacaac
     3181 ctccaaaaca gcctctgtga atattgacgg aaaattttat catttcgtaa gccggaatgg
     3241 ttttcccttt gtatttggga tgaggcatcc gcgtgtaagg caattcataa gaagcatcaa
     3301 gttgtgcgtc aatcattgga ggataaggtg gatttacaac tacccatttg tcctcggtct
     3361 tttgaattaa ccgatctgcc tgatatcggt tcgactgttc ttctattgtt ttgaatgcag
     3421 aagcatattt ttttctgtcg tgcaaacaag tttcaaagga aggcaactcg attgtattcc
     3481 acaaattgtc ttggggaatg ttatccgaca aataaacagt ttggggaata tcggtcaatt
     3541 catgaaattg tttcccgttt tttaactgac ggattaattc ggtcagcggc aattctccca
     3601 ttccataaat aagtaaatcg gcttgagcat cacataaaat actttgtttc aactgatccg
     3661 accaatagtc gtaatgggtc agtcgtcgca gtgaagcttc tattccgcct ataagaattg
     3721 gaacatcagg ataaagtttt tttaaaatat tacaataaac cactgtggca taatctggac
     3781 ggaagccggg ttttccgtcg ggagaataag catcgttgga acgcaaacgc ttgttggccg
     3841 tgtagtgatt gaccatagaa tccatattgc cggccgaaac tgcaaaaaac atgcgtggtc
     3901 gccccatttt tttaaaatca cgcaaatcgt ctctccaatt gggctgagga acaatagcta
     3961 cacggagccc caacgactcc atcagccttc ctatcaaggc attggcaaaa gcaggatgat
     4021 ccacataggc atcaccggta aaaaaaacca catccacatc ttcccatgct cttaattcaa
     4081 gctcttttcg cgtaaccggc aaaaaatcgg tcaattgata ttccatatat cttcacagtt
     4141 tcaacaacaa aaataatgga taattttcaa tttatcctac gacacatgat tattaaaact
     4201 ttcctactca attttttttt caaactgtaa ttgcaaattc gtccaataaa ccaaaaaaat
     4261 tcgagtttct ttaaaatctt tcaagttttt aaaatcaaag aaaaaagtaa ccggaaaaat
     4321 attgagaaaa agattgaaaa accaaaaaaa aattgtacat ttgcaccttc attcagaaaa
     4381 ggttcttaag aagcactgcc ttaggcatct caccttactg atttaacaaa aaaacgaata
     4441 aaatgtacgc aatcgtagaa attttaggac aacaatttaa ggtacaggca ggcaaaaagc
     4501 tgtatgtaca tcgaatggaa gaagccgaaa taggctcaca ggtggagttc gacaaagtat
     4561 tactggtgga caacgatggt tcaatagctg tcggtgcgcc atgcgtggaa ggagcaaaag
     4621 tggtttgtga tgtagtagga cacgtaagag gtgaaaaagt aattgttttt cacaaaaaac
     4681 gtagaaaagg atatagaaaa cgcaccggac accgtcagga tttcactgaa ttattgatca
     4741 aagaaattgt agcttaattt taaaaattca ctaaaaatgg cacataaaaa aggagtagga
     4801 agctcaaaaa atggtcgtga atcggaaagc aagcgacttg gagtaaaaat ttatggcgga
     4861 cagtttgcca aagccggtaa tatcattgtt cgtcaaagag gtacggttca tcatcccgga
     4921 aaaaatatcg gaatggggaa agaccatact ttgtatgctc tggtagatgg aaaagttgaa
     4981 ttccgcaaga aagtcggtaa tcgctcttat gtttccatca atcccgaaat gaattgaaaa
     5041 atttcagtct ctttcaatgc aagagacttg taaacacatg ataatctcct gataatttac
     5101 ccaacggtaa aattgtagga gattttttta ttaaatattt ctcaaacctc aattttttgg
     5161 ccgaagtttt tcggtcgtct tttaactgct aaaataaaaa attgttttta taatgctgac
     5221 acttaaatat attgccgaaa attccgaaga tgttctgaaa cgactgaaaa agaaacattt
     5281 tgatggaaaa gagttaattt caaaagttct ggagcttaac gccgaaagaa aaaaaactca
     5341 atatgacgcc gattccatcg cagccgaaat taataaaata tccaaagaaa ttgggatcct
     5401 ttataaacaa ggtaaaacat cggatgccga gactttgaag caaaaaaccc ttgagctgaa
     5461 ggaaaatatt aaacttcttg aagaaaaaca aaaccagatt gaaacccaac tgaacgaaat
     5521 tctggtgcaa attcccaatt taccccacga aagcgttccc gaaggaaaac ttgcagaaga
     5581 caatcaggtg gaacgtaccg gaggaaaaat tccctcattg ccaacagatg ccttgcctca
     5641 ttgggacttg gcaaaaaaat acgacattat agattttgaa accggcgtta aaattacggg
     5701 agccggattt cccctttata agggaaaatg tgcccgactt caacgcgctc ttatcaactt
     5761 tttccttgac aatgctcgcg aagccggtta tatggaagtt cagccacctt atgttgttaa
     5821 cgaagcttcg gcttatgcca ccggacagct tcccgataag gaaggtcaaa tgtatttctg
     5881 tcaggaagac aatctttatt tgattccgac cgcagaagtt cccgttacca acatttacag
     5941 aggtatgatt ctcaacgaaa acgaattgcc gataaaaaat gtggcatatt ctgcatgttt
     6001 tcgacgcgaa gccggttcgt acggaaaaga tgtcagagga ttaaatcgtc tccatcaatt
     6061 tgataaggtg gaaattgttc aaattcaaca tcccgaaaaa tcgtatgaaa cattttacga
     6121 aatggttcat tatgtggaag gattggttga aaagctcgaa ttaccttggc gaacactccg
     6181 actttgcggg ggagacatga gttttacttc cgctcttacc ttcgacatcg aagtttattc
     6241 ggctgcccaa caacgttggc tggaagttag ttcggtttcc aactttgaaa gttaccaggc
     6301 caatcgattg aagtgccgtt acagaagcgc ggataagaaa attgaacttt gtcacaccct
     6361 caacggcagt gctttggctt tgcctcgaat tgtggctgcc ttgctggaaa ataatcaaac
     6421 tcccgaaggt attcgcattc caaaagcttt gattccgtac acaggatttg agataattga
     6481 ttgatttttc ctataaaaca aaagctaaat ttcaattttt taagattgga tttgtttcaa
     6541 ggcaaatcca atcttttttt ttgtaattat gtttcctcta aaaaggcatt acaaaaaaat
     6601 cggtatgaat cagaaaaaat acgaacaggt attttgaaaa aaatgaataa attccaaatt
     6661 tttatgtaag tttgtagaaa aatcattgta tggaatctat ttcttcattc gctgaactga
     6721 tagcccattt aaaattttta aacaacagaa aacgcgttgc agtagcaaat gcagtagatc
     6781 cacatacatt ggaagcggta ctaagagctt cggataacgg ttttgttgaa gctttcctga
     6841 ttggcgactt ggccatgata gaatctccac acttttttga acacaaacta actccttatg
     6901 tgcacattat cgatattccg gaagtggaag aagctacacg cgaagcggta agaatggtca
     6961 gaacaggcga agccgacata ttaatgaaag gattggtaaa caccgacgtg attttgcggg
     7021 caattctgaa taaagaaaac ggtttggtag aaaaaggaaa aattattacc tacaatgcag
     7081 ccttagaaat acccgattac caaaaactgc tgttttttac cgatccggca gtcatcccct
     7141 ctcctactgt agaacaacgg actgccatga taaaatacgt aacggaaaca gcttacaaat
     7201 ttggaataac ccgaccaaaa atagctttaa tccatgcaac ggaaaaagcc aatccgaaaa
     7261 ttcaatacat gcaggattat ctcgacataa tggagaaatg gcgaatgggt gaatttggcg
     7321 atgttattat ggatggcccg ctcgatattt ttcttgcttt agaccctgaa agaggaagta
     7381 tcaaaaaagt atcttcaccc attcatggcg atgcagatat tttgattttc ccaagttttg
     7441 aatgtgcaaa tgctttctat aaaggattaa tggtttttgc aaaggccgaa atggcaggtc
     7501 ttcttcaggg aacgcaaaaa ccggtagtgc tgacctcgag aagcgaaagc gtcaactcca
     7561 aatattacag tttggctatg gcttgtgtca tggggtaagg acagacttgt cggaatgcaa
     7621 caactcgaag attttttttg cagctcggct tgcggctccg ggttctccca atatctcaga
     7681 aactttttta tactcatttt tcatattttg cctgtaatgc gcatctgtaa gaattctatt
     7741 taattcattt ttcacatttt ctaccgtaaa caaatgagca tacagctcca tcaccacttc
     7801 ctttccggca atcagattga ctaaagaaat atatttcact ttaataaaaa gttttttcaa
     7861 ccaataagcc actttcccaa aggcaacatg ataaacaacc acttgcggag tgccgattaa
     7921 agcagtttcg agagtagctg ttcctgaatt gacaatggca acttcggcat gaaaaagcaa
     7981 ctcataagtt tgaccaaaaa ccacagcaac tgaagtatct tttaaaacag aaaaataaaa
     8041 atctttctca acacccggtg ctccggcaac cacaggctga taatcgggaa actgcattgc
     8101 agcctcaacc atgcgcggta aacaggattt gatttcctgt cgccgacttc cggccaatag
     8161 tgctatcaca ggtttatcga ccaaattatt ggattgacgg aaagtttgga aatttccccg
     8221 ataagctttc ttaaactctt ccaccgcatc cactgtagga ttaccaacat attcgaccgg
     8281 ataacgatgt tttgcataaa actccgtttc gaaaggaaaa atcgtaaaca ttctatctac
     8341 gtaacgtctg atttttttaa ttctaaactc tttccatgcc catatttttg gagaaatata
     8401 ataatatacg ggaatattca atttcttttt tgcaaattcg gccattctga ggttgaaact
     8461 cggataatcg accagtatca gcacatcagg ctgaaatttg agaagcgcct gcttgccatc
     8521 tttgatattt acagaaataa ccgaaaggtt tttcaacaca gcaataaatc ccataaaagc
     8581 catttgccga tagtgtctta tcatttctcc accttcggtc cacattaaat caccgcccaa
     8641 aaagcaaaat tcggcttgca gatcctgctt tttcagttcg tgcatcaaat gcgacgcatg
     8701 caaatcgcct gatgcttcac ccgcaatcaa aaaatatttc atgaaaatat aaaatcaata
     8761 attttagcta tgtatacaaa taatcggaca attacagcat aaaaagactt gcaattaaaa
     8821 aaggcaatcc gcccacaatg gtgcccgaag taagacgaaa agcatcattt ttataaaaaa
     8881 caaaagccat cagcaaatcg ggaacaatgg acaacagcag caacttcccc aacaaaacgc
     8941 ctggataaag acgtttcaaa atttcaaaaa aagatatatc gacaggatag aagcttgtta
     9001 tatagagcca aacgaaaacg cagggcagta tcaaacccac taaaaagcca atataaaaat
     9061 ggttgagacg tttcacggca taagaattta caaattccat tccctgatca agtccattgc
     9121 atcataagct gtcaaatcaa ttttatcggg aacaatggaa ataaaaccat gactgatagc
     9181 ccattcgtcg gtatcctcgg catcgggttc catattttca aaatatccgg taagccaata
     9241 ataagccctg ccggcgggat cggttcttgc agcaaactcc tttgtccagt accccttgca
     9301 ttgacgcgca acacgcacac cgtgaatttc tccgcaggga gcattcacat tcagacaaac
     9361 actttctgga agaccttttt ccatcacaat ttgtgcaatt ttcaatacgt gaggttcaaa
     9421 aaaactaaaa tcaggatcgg gaaaatggct ggccaacgaa aacccgatgg aaggaattcc
     9481 gttctcacaa ccttcaaaaa cagcacccat agtgcccgaa tatatgacat tgatggcaga
     9541 attggagcca tgattgattc ctgaaaccaa taaatcgggt ttacgattca gaatttcatt
     9601 cagtgcaatt tttacacaat cggttggcgt tccggtacag gaatatcgaa ccattccttc
     9661 ctcttcttct atttttctgt aacgaatagg ctgcgtaacc gtcaaagcac ttgactgagc
     9721 cgaacgcgga atatcgggag ctaccaccac tacatctccc aactgcatca ttaatttcgt
     9781 taaaattttt atcccttttg catctatccc atcatcattt gaaatcaaaa tcaaattttt
     9841 attcatgctt cctcattaat ttttctaaca acaaaaattt taaaaatcga caataaaaaa
     9901 tattctttca aaatcagcgc ttacttgtcc gatttggaat ttttccatac aattattccc
     9961 aaaaacaatc cacccaaaaa caagaaatat cccacatcac gcatatccaa cactcctctg
    10021 ctcatagacc tgtaatgagc gtttattcca agattttgaa caaaatcaat cacagctccg
    10081 gaagagaaaa aacttcccaa taagtcgaaa ccgtaaaaga aaataaaaca tagcaacact
    10141 gacacaacaa acgaaataat ctgattgttt gaaagcgatg acgaaaaaat tccaatggct
    10201 atgtaaacac ccgaaagaaa aatcaaccct ataaacgagc cccagaaagc tccggcatca
    10261 atatttccca ctggttcggc caaaaaataa accgagaaat aatacaaaac ggttggaaat
    10321 aaagcaatta aaaccaaaac ccaaccggcc agaaattttc ccaaaaccag ttctgtgatg
    10381 ctcaaaggtt tggttatgag tatttcccaa gttcccgatt ttttttcttc ggcaaaagat
    10441 cgcatggtaa tggcaggaca caaaaacaag aaaagccatg gcgccagata aaacaatcca
    10501 tccacattgg cataacccga ttcaataata ttaaattcgc cgggaataac ccacaaaaat
    10561 aatcctgtca aggccaaaaa aatgccaata accaaatatc ctgtagcact gctgaaaaaa
    10621 cttccgagtt cttttttgaa aatagaaatc ataccaaccg gcgtttttgt ttaaattatt
    10681 tactgataaa gactgcaata attcgctcga ttaaaaaatt tgttaacttt acgctttcaa
    10741 aggtaaccaa aaattgtcaa aggaaaaatt acatttatgg gcagaaataa aaaaaaattc
    10801 ctgttattgc caatgttttt cgcaatctac tcctttggct ttgcgcaaac gtttaaaaat
    10861 tattctctga gtgcagattt catttacggt gaaattctca agcacaacaa gcatctcgaa
    10921 aatctggtca aaggaccgct caaaggcgaa gaattttcaa tagaatggca aacaatggga
    10981 gaaaaagcgt ggcatcagta tcttaatttt ccttccgttg gaatttccac agcttttttg
    11041 gatttcagtc atcccgacac tttggggtat gctgtagccc tatatccata tttgaaattc
    11101 ccgattgttc gtcagcaaca ttttaatatg gctttcaaag ccggcggagg gctgagttat
    11161 gttacaaaaa cattctacaa tgctacagct tatcatcctg atggcagcgt atatctcaac
    11221 aaaagcaatg ccgctatcgg atcacatgtg aatgtctatc ttacagccaa tctcaacatg
    11281 gaaattcctt tgacaaaagg atggagcatt acttccaact ggggatggaa tcatacttcc
    11341 aacggaagca ttgttcaacc aaattcagga atcaatatgt tggatatgta tttgggaatt
    11401 aaatattttc caaattacga gcgttatatc ccacctaaac ctcaaccatt acctgacctg
    11461 ccgcataaaa tcgaaattga aatggctttt accggcggca tacgacaact ttattacaaa
    11521 gacgacaaaa cctatcccat cggcactttc aatatagcag cataccgaac catgaccaat
    11581 ttttacagaa tgggatgggg cgcagatttg ttttacgatg gcgtttttaa tggtaaatca
    11641 gctttcaaaa gaacttatct cactaccgac gagttaaaat acaaactcag aattggtgct
    11701 tcttttcgac acgagctttt agttgggaga ctcaccacag gtttccattt tggcctctac
    11761 ctttataatc cggtgaaaaa tctcgaacct tacgaagaag ctaaaaacgg aactttaaac
    11821 aaacctttga tttacaaata taatattgaa aaggaagatg gttggtttta cacaaaagct
    11881 gtattaaaat atgctttgga cgaacatttc ctcttatctt tgggattgaa aacccatttg
    11941 caaaaagcgg aatttattga tgcgggaatc ggatataaat tttaaaaagt tctccgacca
    12001 tttctattca ttttcgaaat atagcaatgc atccatcatg agcggcaatt cttcttcccg
    12061 aataccaaac gaaatcaacc tgtccttatc ttcattaaac aaatcctgaa aaacttccca
    12121 tttattctgc aacatttcta aagacttgcg ataagcatcg gctgctgcag acaactgacg
    12181 ccggcaccaa ctaatatgac cggaaagcaa gtaatcgtaa gcattgggtt cagccaaaat
    12241 aattttttct gaataataat cggctttatc cagatttccg tcaaaaaaat tgcattttgc
    12301 aatcgctctc cacactttta tgtcgtccac attttcggca tccagtttat aataaattgc
    12361 cagcgcatct ttaaattttc cgagttcaaa ataacattga gcgatatgta tttgcgtact
    12421 gtactgaccg ggtttcagaa aatcggcatg ttgataaaat gccaatgctt tttcataatt
    12481 tccaagcaac cgatagcaaa gcgcaatttt tcgaatagtc cagccatcgt ccggttgaat
    12541 catatcggct ttcaaatagg catccagtgc cttttgcaat tgcgaagtct gctgatacgc
    12601 ataacccatt ttctgataca acgcagcgga aggctgactc tccgactgaa ttttttcata
    12661 aagctccagt gcctgcaaat aatacccttt agaaaaataa tattcagcta cactttccga
    12721 aaaaccggga atagaagaca aaatatcgaa cagccaggtg cgatgtaaag tcagagaata
    12781 ggaaaacata tcttcaaaat cgtccttgtg aggctgaagt ttaaagaaac ggaacaaatc
    12841 ctgaatgtac tgtttggaaa tgttttttgt aattgtttcg ggcgaaagta tcgacatttc
    12901 ttcttccatt tcgtccattt gatccgattc cattttaaaa gaatgtttaa gcatatttcg
    12961 ttggctttcg ggcatttgta acacactcag gcaaaatgaa tatttgtctg aattgcacat
    13021 gacattgctg ttgacaaaag ccgacaaaat ggttttttca tccgactcga acaactgatt
    13081 gactgccgaa ttttcagtat cgaaaggcaa gaaccaatga gaaaattgag agaaaaatgt
    13141 aaaatttttc agcatggaaa aagtactcat gtaaacatcc gctccttcca attgtaaatc
    13201 gctcaattct ttgagtttat ccgacaagcc actttcatcg aaaatttcct gccattccgg
    13261 atttgcttct gcccattcat cggaattcaa aaagttttca gcatccattt tgtctctcaa
    13321 caaaggattg attttcatca tttcaggtaa aatttcttcc ctgagtttcc gcgaaatttt
    13381 ttctgtttcg gtagtcccaa taatttgaat aatgatattc tgaaaatttt cggcaatacg
    13441 gttttgatcg actaaaagaa ccaaacgatt acgaatggaa gggaaataag gcagataaat
    13501 attgtatttg gccagtataa aagttaaccc gaccaatgct cgctgaacaa ctttttgatc
    13561 gtcgttttcg cagctgtcaa aaagaagcat tattttttgt tcgtcaaatt ttctccacaa
    13621 attcaacgtt acggccgaaa ccatcaagga tttttccacc cagtatttat catcggcttc
    13681 gagcaatttc ttgtacaaat tgttttcttc ttcggaatag cgagttgcca gccaaaacac
    13741 gccaaaaagt tcatccagcg catcttcaaa attggatcgc attctgctca attcaatttt
    13801 gtactgttcc ttattttcgg aaagcaaagc cgactgcttg tggaaatact gcaaagcatt
    13861 gaacagctca ttgactgtct gatgtttacg tgtgtaaggg aaaaatcttt tctgtgaata
    13921 ttcgaaattt gaagaatttt tcatcatcag ctcttcgcga acgtcagcaa gcagtatcaa
    13981 aagttttgcg accaacttac tgtaaaccga tttcctttcg ggatcttgca cacccttgag
    14041 ataatactcg agcaaaaatt tgtaattcct ttccaattcg ttaaaacgat ccgaaaattc
    14101 gccgtactgc aaatcgtcaa tcattatttt tatcctgtcg aatgcaggtt tgagccttga
    14161 cgccgacaca aatcgcaaaa tagccttata agtagtatca atttgagttg cattcataaa
    14221 taaaaacaat ggtcaattaa atatcacttt ttcaatttgg agaaagaatc acaaagcgaa
    14281 aaacaacaaa aattatccca ttttacttat ttttctgagt ttatcttcat cgataatctt
    14341 aatttttctt ccatccacag caatgatgtg ctcgttggta aaattcgata aagtgcgtat
    14401 agcattggat gtggtcatat tcgacaaatt tgccaaatct tcacgcgaca aatagatatt
    14461 gacggtagcc ccatcttctt ccactccgta tcgttttttc agaaacagca aagcttctgc
    14521 caaacgacca cgaatgtgct tctgtgtcaa attgacagtt cgtgcatcgg aaactgccag
    14581 gtcggaagcc aattcttcaa gaaaacgata gcaaactgca atattgtgtc tgagaataga
    14641 caggaacaaa tcggccttga gcatataaac ggttgaggct tcaaaagccg ctacattggt
    14701 gctgtttggc tcattggcta taatggcacg ataaccgaaa ttttcgcccg gtttcaacat
    14761 tcgaataatt tgcgttcggc taccaatacc atccttgtaa acctttgctt tacccgccgc
    14821 caaaatcatc atgtgggtag aaatttcacc ttcgctttgg atcagttcgt ttttcttgaa
    14881 atgatgaacc gtgtagttgt ttcttacaaa ttgcctttcg tcgggcgtca gcacatccca
    14941 aaccatgaaa acttcttcaa tgggtttcag ttcataagca ggttttttgg tcattgcttc
    15001 aaatgaaaaa atatgtttga caacattgtt atacagcaca aatttaatta tttttttaaa
    15061 ataaattcca aaacgtaaaa taaaaatatt tctctattcc gaataaaaat tctaacgggg
    15121 aaaaattaaa aaatttctga attgtaaaca tttgaaacta ccagtaaaat gtagaggaaa
    15181 acattgaata aaacttttaa caaaaagcta tgagaaaaga aaaatttatt acttttgcca
    15241 tttgaaatcc ataaaaacca atcgcaatac atgaaaagag caatattcgt ttctctcctc
    15301 agtttcatct tattcccaat attttcacaa aatctgacat tcagcgattc ggcaaaagta
    15361 agtttgctca cttgttctcc cgggaaaaaa gtgtatgaaa aattcggaca tactgccatt
    15421 cgtgtcaacg acccgattac cggctacgat gtggttttca attttggcgt attcgatttc
    15481 aacaccaaaa acttctacct gaaatttatt aagggagata ccgattatca gcttgccgta
    15541 tatgatacca aaagcttttt ggaagaatat aaaagtagaa attcaatggt ttgggaacaa
    15601 aaactgaacc ttactttaaa cgaaaaaaga aatctgcttc actctcttat aaaaaattac
    15661 gagcctgaaa acagattcta ccgctacaat tttgtttatg acaattgctc tacccgacca
    15721 agagataaaa ttgtacaatc ggttaatgga aaggttgaat atcatttata taacgagccc
    15781 gtaactttca gagaaatgat agctcaatat accggagtaa atagttggat gcaatttggt
    15841 cttgatctaa tcttcgggcg tgatgcagac aagagcgttt cgcgaaatgt atcgatgttt
    15901 ttacctgaaa atctgatgta cgaatttcag gcagctcata taatagctgc agatggtgca
    15961 aaacgccatc tggtagctga aaataagata ttgattccca aacaggaaga aaaatattct
    16021 aaacctttgc ctatatttca gcctgtattt gtttgtactt ttcttttaat tttgggaatt
    16081 atcataacta ttttcaacaa taaaaaaagt ttccgtttgt tcgatagcct actttatata
    16141 gtaagcggtc ttggtggaat tattgtcttt tatcttgcct ttttctcgtc gcatccgctt
    16201 gtaaaaaaca attttaatct tctgtggctg aatccgctga attttgctct gggcattctc
    16261 ctttggtttc acaaaatgaa accgtatgtg ttttattatc aagttttaaa ctgtttattg
    16321 atttttctgg ctttggtagc ttatgctgct ggacttcagg ttttccatcc ggccagtttt
    16381 ccgcttatcc tgttgctttt gctgcgaaca gtgcgatgga cagccacaac aaaaaaagat
    16441 ttttcgctaa agaaaaaaaa taatcgggac tattacacca atagcaacgg actacaccac
    16501 cactcaagga aaagttgaga taaaatttct ttaaaaaaat catttgacca atgactctcc
    16561 attctaataa aaattttcgc tttgtcggca ttattccggc ccgctttgct tccactcgtt
    16621 ttccgggaaa accgctggca gatattggtg gaaaacctat gattcagcga gtgtacgaac
    16681 aggcacgcaa agtattgcaa accgtttatg tggctacnnn nnnnnnnnnn nnnnnnnnnn
    16741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16801 nnnnnnnnnn nnnnnnngaa gaatacgatt acatactgaa tcccaacaga cctaaaatag
    16861 tactcaataa aaacaatgaa gccctgtatt tcagcaggca agttattcca taccaacgaa
    16921 atactgccaa cgaacattgg atttcgaatc atcagtattt tgttcatgtg ggaatttatg
    16981 cgtatcgaac agagatattg aaagaactct gttcacttcc cgtttcatcg ctcgaaaaag
    17041 cagaatcatt ggaacaactc cgctggctcg aaaatggata ccgaataaaa gttggcttca
    17101 caacagccga atcttatggc atcgatacac cggaagattt ggaaaaagtt agaaaaatgt
    17161 ttttgggcaa ataatcacct tccttttttt tttcaaatgt aagggaaagc tcttcattac
    17221 gaaattttct gataaattgg ataagtactt tacgaaaacc catcctattc tcgcacgatt
    17281 ttatcgtgtg ggtggtctta aagccgatag atagcaatac aattgaaaat gccttaggga
    17341 aaagataggt tgaaataatt ttaattagct acacaatgca atcatgcggc aaccaagtaa
    17401 aaaattatga tcagaaaaca gaattatata catcattttt ccattgtggc aacagttggg
    17461 taaaaaatcg cttaataaat tgtaaccaat tgattaaaaa ctacaaattc aatttgacga
    17521 acggctcaat gctattttaa attcattcaa aaatctgcct ttcattcaat gcccgctgat
    17581 attttcgagc attgatcaaa tgctgagcaa gtgtggcggc aaaattatgt tcgccattca
    17641 acgtttcttt ggcacacata taaagatagg aatgatgtga ataattcaaa acggcatcaa
    17701 tcacctgagg cgaaggcaat cgaatgggac cgggtggcaa accttgaaat ttgtaggtgt
    17761 tgaagggcga attgatttgc aaatgttgac ctttgattcg tttcaaagaa aaatctttcc
    17821 atgcaaattt tatggtagga tcggcttgca agggcatttt attttttaac ctattgatat
    17881 aaactcctgc aataataggc tgttcatacg atttgttgga ttcttcttct acaattgatg
    17941 ccaaaatact gacttctatt ggagtcagcg gtatggaagc agctttctgc cggcgttctt
    18001 cgttccaaaa ttttaaatac tctctgtaca ttcgatcgaa aagttttttg ggtttaatat
    18061 tccagaaaac ttcgtaagta tccggtataa atatcacaac cgaattatcg ggattcaaag
    18121 cgtattgcga caaaaattga gaattgttca gcaattttaa aaaggtgagt gaatcggtca
    18181 ttagttgaga tgacaatcga ccggccaatt gttcttttgt tcgtatattg ttgaatgtca
    18241 atcgcaatgg aacctgtctc ccactcctta agtttcgaat taatgcaaaa ttgttcattc
    18301 ctttttttaa cgcatagcgc cccgaacgga tatttttatc gtaatcgaaa attttggccg
    18361 ttaaccgcaa agaataacgg tcccacacgc gtgattcatt tttgataagt ttcacgacat
    18421 cttcaaaact cgtgttctcg ggaatgcaaa caaaagtttc tgtttctttt tttggaaaaa
    18481 tattgggcgc aaaaaaaacg taggtcaaat aaacaaccac aattagtgga accgcaagcg
    18541 ctgcaaacaa aattttcctg actttctttt tcataaaaaa tgcgtttgga tttgagttgc
    18601 aaaagtaaat gtttttctta aaaaaaacac ttatccgttc taaaatgtaa ttcattaaaa
    18661 atcaatttga attgttaaat tcatcttttc ttgaaattat tccacacaag cattgagtaa
    18721 aaatgacttt gataatcgaa aactctttct tatctttgca ttacaaaagc agacacaagg
    18781 aagtgtgggt gagtggctga aaccatcagt ttgctaaact gacgtacggg tttccgtacc
    18841 acgagttcga atctcgtcgc ttccgcttta tattgccgga tacttttcac cagttttcgg
    18901 ctttttttta agacaacaca cacaacatca atccgattaa ttttatcgtc tctcaaattt
    18961 taaaattact gcaatggaaa aaacaaaaaa atttattctt accgaaaagg aacttccaac
    19021 ccattggtac aacattgtgg cagaaatgca gaacaagccc atgccaattc tgaatcctgc
    19081 taccaaacag cccatgaaag aagaagaatt gtacccactt ttcgcagaag aattgtcgca
    19141 tcaggaatta aatcagaccg atgcatggat tgaaataccc gaagaagtac gcgagaaata
    19201 taaaatttat cgacctactc cgctggtaag agcttatgat ttggaaaaag cgctcgacac
    19261 acccgcacac atttatttta aaaacgaaag tgtcagtcct gttggctccc acaaattaaa
    19321 cacagctttg ccacaagctt attattgtaa acaggaaggc gttacccact tgactaccga
    19381 gaccggcgcc ggtcaatggg gaacagcgtt ggcttttgct gcgaaaactt tcggtttgga
    19441 attgtttgtt tacatggtaa aaatcagtta cgaacaaaaa ccttatcgtc gctcactaat
    19501 gcaaacttgg ggcgctcagg taattgcttc tccaagcatg tccaccaatg caggacgaaa
    19561 aattctgtcc gaacatccca actatctcgg aagtttggga actgccattt ctgaagccat
    19621 agaacttgcc atgaatacgc caaactgtaa atatacgctt ggcagtgttt tgaatcatgt
    19681 ctcactgcac caaaccgtaa taggactgga agcagaaaag cagatggaaa tggcaggcga
    19741 atatcctgat atagttattg gctgttttgg tggcggttcc aatttctcag gaatatcttt
    19801 cccattttta cgtcataata ttaaagatgg taaaaaaaca cgttttatcg ctgccgaacc
    19861 ggcttcttgt ccgaaattga cacgtggtgt ttttgaatac gacttcggag atgaagttgg
    19921 ttacactcca cttttgccta tgtttacctt aggacataat tttgctccgc ctcacattca
    19981 cgcaggtggt ctgcgttatc acggagccgg cactattgtc agccaattga aaaaagacaa
    20041 cttggtcgaa gcagtagata ttgatcaatt ggattctttc aaagccggcg tacttttcgc
    20101 acaaaccgaa ggaattgttc cggcgccgga atcggctcac gcaattgcag tggctattca
    20161 tgaggctctg aaagccaaag aggaaggaac accaaaaact attttgttta atctttccgg
    20221 acacggaatg gttgatatga ctgcttatga ccagtatttt gctggcgact taaccaatta
    20281 ttccctttct gaaaacgaca ttgccgaaaa tataaaaaat ctggaacatt tggcaaaata
    20341 aacaaatctg aaaattaata cgaaaaacag gacagtgaaa aatttcacag gtcctgtttt
    20401 tttataacca aaaggtaaga atttatcttt ctttcatcaa aaaacgacca atacggcgcg
    20461 tacgctgcat aagcaaaact tctcgatgat ttgtaacttc ctgtgcaatt tcatccgaaa
    20521 ttcctctgac agtcagcaat tctaaatttt cgttgtaact tacatcaaac tcattcgaca
    20581 gttcgtccaa agcagccggc aaatatcgac tgttgccaat ggctaccgaa atgctgatgg
    20641 ctgagctttc aatcatcgaa acttttagcc ggtgtttatt gatcatggaa aaaacttttg
    20701 gcaaattttc ttccagcaca aaagaaaaat ctatcggacg aatggttacc aacacttgat
    20761 ttttcaacaa actgtaaaac ggaataccgg ctggtttttc gacatgagct ttaataacag
    20821 aacccttttc agaaggattt ccgaaaggac gaacataaag cggaatattt ttattttgca
    20881 aaggtttaat cgttttggga tgaatcacct gagccccgct ataagccaat tccaccgctt
    20941 cggaataagt cagttcggga acaagctttg cctgaggata aagacgtgga tcggcattca
    21001 aaatacccgg aacatctttc catatagtca aattttctgc attcagcaag ttggccacaa
    21061 cagcagccgt ataatccgaa ccttcacgtc ccagagttgt cggatctcct ttggtattgg
    21121 ctccgataaa accctgacca atataaaccg gataattttc gaaatttacg gcttccgtca
    21181 atttttgttg cgattctttc atcttgacat ttgcttccct aaaattgctg tcggttatca
    21241 gcagtttccg catatccagc caaccatttt gaataccggc ttcatttagg aatttggaaa
    21301 caatttgcgt tgaaattatt tcaccatagg atacaatttt atcgtaccat cggtcataat
    21361 cgtcaccaac cgaaagtttc agcatactgg taatctcgtc gtaaagaggc tgcaaaatag
    21421 caacaggatt tgctgaatcc ggaaaaagtt ctgctaaaat ttcattgtga taattttcta
    21481 tttgttgaaa ttttgccaga gcagaagcgt gatcggcctg cataaaatct tccaatacac
    21541 cttcgagcgc attggttgtt tttcccatcg ccgaaacaac aatgaataaa ttatcattca
    21601 cgccagaaac aattgatgcc agatttttta ttccggctgc cgaacgaacc gaagcaccac
    21661 caaatttata tacaatcatg aaattatttg taaaattact gaataaaaat acctttctta
    21721 ttcatttaaa tggactgcaa aagtaaaata atccgaaaaa ttacacaact gtttggagat
    21781 actatttgaa aaattcaaac agataggcaa taatacgaaa aagaaagaga aaaatattta
    21841 tatcttttca acataaattt gaccggtgcg atattcaagc acgcgaacct gaactccttt
    21901 ttcaataaaa ccttccgtag aaacggcatc ataaactttt ccttcgacat ttacttttcc
    21961 tgaagggcga agaaccgttg aagcataacc tgttttaccc accagctgca attcatccat
    22021 tgaaacgcct atgtaacctt ctttattttc gagcgccgta ttgagagcca tttttttgaa
    22081 aattcccgtc gaaccgattt tattgctcaa atacaatatc aaaacaaatc ccaacgtaat
    22141 gccaaccaaa acggtaagca acgattttcc aactgtgcct gcctgaaccc catcaaaatt
    22201 gaaattgaca ttgtttatta aactgagcgt taaacccgcc acagagaaca aaattccgga
    22261 aattccggct ataccgaaac cgggaattac aaatatttcc aatgccaata gaaccagtcc
    22321 cagcaaaaaa atcaaaattt cccaattggc agccaaacca tcaatataca aaggaacaaa
    22381 aaacaatacg gctgcaagaa tacttattgc caagggaaat cctatgcccg gagtctgaag
    22441 ttcaaaataa atacctccaa taattagcat aattaaaatg cttcgtacaa tggagctgga
    22501 aaaaaatcct ttcactttgt cccaaaaact cggttgaaaa acaataatct cgaaattagc
    22561 ttcagtaatg tgatttttaa taatttcatc aatactttct gcaattccat cacaatatcc
    22621 gtgttgcaaa gcttcttctg ccgtaaaagt caatgttttt cctgcttcaa tgataccggg
    22681 aatcgacacc ttatcgtcca ccattgcttc tgcaattttt ggatcgcgct tccattgaaa
    22741 aatggtatca ttaccctgaa taatggtatc tttcccgtga gcttcggctg ttgtacgcat
    22801 ggttgcccgc atgtacgact gatatttatc gggcatttgc tgtccggtgg tatttacaac
    22861 tgttgctgcg ccaattgccg ctccgggtcg cataaaaatt ttgtcgcaag ctatggctat
    22921 cagagctccg gcagaagcgg cattgttatc gataaaaaca taaaccggaa tcgggctgtt
    22981 caaaattcgt gttcgaatgg aatcggcaaa aaccacttcg cctccataag tattcatgtg
    23041 catcagaatg acatcggcat ttttctcttt ggcttgatta aatgcctttt gacaataaac
    23101 ccatgctgta ccacctatat cttttttcaa ttctataaca aaaacagtcg tttttttatt
    23161 cgaagcattc agattttcag ttataaaaca cattgtaatt gttacaaaaa tgtaaaatat
    23221 ataaaacttt ctcatattat cgaatatttt tgtccaaata tacgaaacat ttaaaaatat
    23281 gctgtatatt tgtcgtgaaa atctagaaca agtattttcg aaagagttct ttgttaagtt
    23341 tttaatggaa gtataaattt gatgctattc gattcaaaaa ctttttcaga aagattcgtt
    23401 ttagaataaa aaattacctg ccgaattttt ttgtcggaga ggtgaaattt attcacacac
    23461 ttaatattaa aagttatgaa gccaaatcaa tttgaaaacg aattaattgc cttacaaaac
    23521 aatatgcgga atttcgcttt ttcactcacg ttgaacagag aggatgcaga agatttattg
    23581 caggatacca ccctgaaagt attggacaat agggaaaaat ttaccaaaaa cgtcaatttc
    23641 aaaggttggg tgctgaccat aatgaaaaat atatttatca acaattatcg taagattgtt
    23701 cgcaatcaga cagttattga taaaacagaa gacatgtatc accttaattt gccgcaaaat
    23761 tccggatttg acagccctga aggctcctat tccatcggtg aaattaacag caacattgaa
    23821 gcatttcccg acgaatacag aattcccttt tccatgcatt tgcagggata taaatatgaa
    23881 gaaatagccg aaaccatgca tttgccaata ggaacggtga aaagtcgaat ttttttagca
    23941 cgtaagcgac tgcaacaaca attgaaagat tatcgcgact gacatctgaa atattttata
    24001 agccggaaaa catttttccg gctttttttg ttttattaaa ttaaaggaca attaaatttt
    24061 atcatttaac aaatgagctt acagtttttt taaaaaagtt ttatgaatac cgccatcgtt
    24121 atacttggaa gcaatgttga ggctgatcaa aatatcgacg ctgcaaaaga aaggttgtcg
    24181 gaatattttg aaatagactt atgcagttcc cgcattggca gcgatgccgt tggtgaaaac
    24241 tatgttgcac catttttgaa cgaagccgtg aaactttatt ccgatgaaac agccgaagag
    24301 acaatagcca ttttcaagca aatagaaacc gaaatgggcc gaactcccga gagtaaaata
    24361 acaggacttg tcccgattga tattgatttg attttctgga acgacaattt aatgcgcaac
    24421 gactacaacc gcttctcttt tgtaaaaaga tgtgttgacg aaatcaagta atcaaatttt
    24481 ctctttgtcg gtttagggaa ttaatctttg caaattcaga aacagaggcg gaaaacgtac
    24541 tttggtagcc actatggaag ctaaagcgaa attcggaagc aatgaaggaa atcacgaaaa
    24601 aattttatcg gtaaacattc attcatgttt aaatctactc cgaaaagttt tatttttgaa
    24661 aattcgcttc gcccccgacc aaaaagaaaa ctcgtttttc agcaacttat aagaaataag
    24721 gttaaaaagt cgatgaattt agttttttga cttttcgaag cggactcatg ttttaattta
    24781 a
//
LOCUS       DAOSIB010000024        24670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_2257_length_24670_cov_12.518505, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000024 DAOSIB010000000
VERSION     DAOSIB010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 24670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24670
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2257_length_24670_cov_12.518505"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /gene="murI"
                     /locus_tag="PLD44_03705"
     CDS             complement(<1..784)
                     /gene="murI"
                     /locus_tag="PLD44_03705"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOF98476.1"
                     /translation="MNELQGNKNYSLTKLELSSLPGPIGVFDSGYGGLTILEKIRQRL
                     PHYDYLYLGDNARTPYGTRSFEIVYKYTLECVVRLFELGAHLVILACNTASAKALRTI
                     QQNDLPKIDPRRRVLGIIRPTVEIIDKLTHNGHVGLLATPGTVQSNSYGLEIQKLYGN
                     AITLSSEACPMWVPLIENFEHNSPGADYFFQKNINHLLQTDPQIDTVILGCTHYTLLQ
                     DKIKNLLPPNINIVSQGEIVASSLDDYLNRHPEIDALCTKNAS"
     gene            complement(815..1783)
                     /locus_tag="PLD44_03710"
     CDS             complement(815..1783)
                     /locus_tag="PLD44_03710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOF98477.1"
                     /translation="MAKKKYHFNPDTLSYEPIENSLWFKIRRFLFHAFTGIATGTVFF
                     FILASTVQSPEERELNAEKNKMEAQYKVLQRQFNEVQDVLTDLEERDNNLYRVVLQAE
                     PIPFTVRKAIASNTDYYNQLYDMTNSEIVVSTTKMLNTVKKQLYIQSRSYDELVNLTK
                     NREKMLQCLPAIQPVLNKDLKRMASGYGWRIDPVYHTRRFHEGMDFVAPTGTDVYATG
                     DGTIVNAGWEQGYGNCIVINHGFGYETLYAHLSTIKVRPGQSVKRGDIIGLVGSTGKS
                     TAPHLHYEVHYKGQVMNPQNYYFLDLSPEEYDRMVQMSNNFGEVLD"
     gene            1987..4614
                     /gene="alaS"
                     /locus_tag="PLD44_03715"
     CDS             1987..4614
                     /gene="alaS"
                     /locus_tag="PLD44_03715"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOF98478.1"
                     /translation="MTSQEIRQSFLDFFAEKGHKIVPSAPMVIKDDPTLMFTNAGMNQ
                     FKNIILGNDPVKYPRVANSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     KEAIEWAWEYLTEVLKIDKSRLYVTVFEGSPSEGLERDEEAAAIWSQFLPADRIINGS
                     RKDNFWEMGETGPCGPCSEIHIDIRSEEERAKLDGLSLINQGHPLVIELWNNVFMQYN
                     RKADGTLEELPAKVIDTGMGFERLCMVIQGKTSNYDTDIFQPIIREIGKISGTEYGKD
                     EKIDIAMRVVSDHIRAIAFSIADGQLPSNMKAGYVIRRILRRAVRYGYTFLHQHAPFM
                     YRLIDVLCETMGKAYPELISQKTLIEKVIKEEEESFLRTLETGIRLLERKMEEAQLEN
                     KKEISGADAFVLYDTFGFPIDLTELILRENNFTVNMNEFHAEMQKQKERARNAANIET
                     GDWIVLREGDSHFVGYDCTSCETNILRYRKIKQKNQEFYQLVLSVTPFYAEMGGQVGD
                     SGWLVGENEKIEILDTKKENNLAVHIAKQLPSEITGTFTAVVHLENRQATSCNHSATH
                     LLHEALRTVLGTHVEQKGSYVGPDSLRFDFSHFQKMTKEEIRAVEALANRKIRENLPL
                     NEMREVPVSEAKEMGAMALFGEKYGETVRVIQFGSSKELCGGTHVKATGEIGMVRIIS
                     ESSIAAGIRRIEAITGKAVEKLMDEQQDILSETREILNNPGNLVNAVRKLMEENTELK
                     KQVNTFQQEKLLQLRDRLMAEAKITNGIHVVRYEGNLTADQAKTLAFQIKNLQNENLF
                     FIAGNVMDGKPSLTLLLSDDLVSRGLNAAQIIREIAKEIQGGGGGQPFFATAGGKNAA
                     GLSAAISKALKFIDEFPSK"
     gene            4882..5715
                     /locus_tag="PLD44_03720"
     CDS             4882..5715
                     /locus_tag="PLD44_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOF98479.1"
                     /translation="MKRVAIQGISGAFHEIAAREYFKGEEIEVIPCETFKDVFKAIEA
                     DKSLLGIIAIENTIAGSLLQNHNLLRESGCLVVGEQKLHIEHHLVALPHQKLEEIEEV
                     HSHPIALMQCEEFLESHKHLKAVESEDTALSAKEIADGQIYKRAAICSLFAAEKYGLE
                     ILERNIETNKRNFTRFLIVAEPTLALEMTKNDEINKASLVFTLPHVEGSLSHVLSIFA
                     FYHMNLTKIQSLPIIGKEWEYQFYINLTFNDYSRYKQAIDAILPLIDEFQIMGEYHAE
                     N"
     gene            5743..6915
                     /locus_tag="PLD44_03725"
     CDS             5743..6915
                     /locus_tag="PLD44_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOF98480.1"
                     /translation="MLKQLKMEIEPARRVNNVSEYYFSTKLKEIDEMKAAGKDVINLG
                     IGSPDLPPSEVTIEALCKEAHKPNVHGYQSYIGIPELRNGFAEWYKKWFGVELNPNTE
                     IQPLIGSKEGILHISLAFLNPGDGVLVPNPGYPTYTSVSNLCEAKVIPYDLDENHEWQ
                     PDFDALEKMNLEGVKLMWTNYPNMPTGANASVELFEKLVAFGKKHNIIICNDNPYSFI
                     LNDRKLSILSVDGAKDICIEMNSMSKSHNMAGWRIAMLASNRQFVEWVLRVKSNVDSG
                     MFRPMMIAAAEALKNPKEWHDQINVVYARRRNIAFKILDLLGCTYDTRQVGLFVWGKI
                     PDRYKDAGELADEILYGKNVFITPGFIFGDKGNRYVRISLCCPEPTLEEVVRRIEH"
     gene            6967..8031
                     /locus_tag="PLD44_03730"
     CDS             6967..8031
                     /locus_tag="PLD44_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HOF98481.1"
                     /translation="MKFESILLPGIEAKRPIVIAGPCSAETEEQVMETARGLASIGIK
                     IYRAGIWKPRTKPGGFEGVGEKGLPWLKRLKSELGLYTTVEVANAKHVRAALAHDIDI
                     LWIGARTTANPFAVQEIADALEGVDIPVLIKNPVNPDLELWIGAIERIYNAGIHKIAA
                     VHRGFSSYDKKIYRNLPQWHIPIELRRQMPDLPIFCDPSHIGGKRELIAPLSQQAMDL
                     NFDGLMIESHCNPEKAWSDAAQQITPQVLEFILESLVIRDTMQTTENLSALRRQIDEL
                     DNNLLELLAHRMRVAEEIGRYKKEHNMPILQAQRYDEILHNRVEQAIEMGMDGEFMKT
                     VLIAIHEESIRHQMEIMEKS"
     gene            complement(8115..9215)
                     /locus_tag="PLD44_03735"
     CDS             complement(8115..9215)
                     /locus_tag="PLD44_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98482.1"
                     /translation="MKTKILGLAALLLFATQTPAQRTFTVEARNYDISDNLDLRAVAS
                     IFAESRDLSDFEMRLNDARYQISNLDLNNDGWIDYLRVVETSGGNLHLVVIQAVLDRY
                     TYQDVASIVVEKRNSPQVYVQIIGDPYLFGVNYVIEPIFVRQPVIFSFFWSKNYRPWH
                     SPYYWGYYPRHYRPLRPVEVNIYLGHVHHIIPRDYKFHYHNSVNIYYRDAYVRLHQTV
                     RRNDFERTHPERGFNSRNKNVANKAELNNSSRRSSTEQERRIVNQDNGSRRISTSSSD
                     SRYNNSENNSSRRSQNTGRVETQNNARRISNSGRSVETRTSSQNEKNSSRTETLRRSE
                     NSTVNTSQRTSENNSRGTRSTNSNSENRSGRR"
     gene            9472..10014
                     /locus_tag="PLD44_03740"
     CDS             9472..10014
                     /locus_tag="PLD44_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOF98483.1"
                     /translation="MNTLDLVILVTVFFGIIQGLFRGLVKELTSFAVIIVGIYGAKLL
                     SPLIAKMLESVASFSPQMAHPLAFLFSFILLAIAMFLLAQLFEKTLSIIQLGGLNKFL
                     GAIVGGLKFLLVLSVLLNVFDAMDERFHLINKATKEESLTYSLVLKAGPELWKEVKDK
                     QSINEQNTHQQNKIVPRQNK"
     gene            9962..10615
                     /locus_tag="PLD44_03745"
     CDS             9962..10615
                     /locus_tag="PLD44_03745"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOF98484.1"
                     /translation="MNKIPISKIKLYPDKTNRKQPSLIRSFSSFQYECGCDEAGRGCL
                     AGPVVAAAVILPPDFICPQLNDSKQLSPLLREKFRKIIEKEALAWSVASVDNREIDRI
                     NILNASILAMHKAIDGLQIKPQFIAVDGNRFKPYADIPFQTIVKGDSKYLSIAAASVL
                     AKTHRDELMEILGEKFPEYEWKKNKGYPTAEHREMIRRYGCTEYHRLSFRLLKEEPF"
     gene            complement(10647..12911)
                     /locus_tag="PLD44_03750"
     CDS             complement(10647..12911)
                     /locus_tag="PLD44_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOF98485.1"
                     /translation="MRNIKIRLKIETIAVALLLTGCNTTKFVPEGEYLLDKVNINIDT
                     RNVKKIELKEYLRQTPNSSVFGLFRTQLGIYSLAGKDTSKWINKTLMRIGEEPVIYNP
                     ALTEISSQQLQLFMHNKGFLRAKVENKVTLKDKKAEVEYQILAHQPYRIRNYSVQLNH
                     PELQAIAADTSRSLIRPGMLFDVDMLDAERERITSRFRQTGYYNFSKDFIVYSADSIE
                     KTHQVDLSMQLRDYLNDKNDSLSQIIFRKFYVRNVYFEINPTDLPSLNQPMEKDTVWF
                     RNFAMITPHKQIIKIDALVQNTYIDPQALFNDANIEKTYSGLNSLGAIKFVNIGFEQV
                     DTGLLDCHISIIPGKAISLSAEVEATYTDGYWGGATNLNLVNRNIFKGAETLSLQVHG
                     AYELQEKVWAQEWGGQIGLQFPRVVLPFASYDFKRNLRANTEFSGSYYYQFRPGEFRT
                     SNLGAGMRYFLNRRQYTHIFDLFEVNYVYFPEIFPAFRDSFLTTGIFNSYNYENHFIM
                     RMGYSGSSTNFNANQPLRNYRTMRYSIETAGNILYGLDHLLGSRPAEDGNYRLLNIAY
                     AQYFKTEYNLTYHQIFDENNRFVYHAGLGIAIPYGNADAVPYEKRFFSGGANGVRGWS
                     ESTLGPGVYKRINTQRRDFNQTGDIKLDMNMEYRGKLFWLLESAVFMDAGNIWTIKDY
                     DTQQGGLFKWDSFMNQIAVAYGVGLRFDFSFIVARLDMGVKLYDPVLKRSDRWRVSPQ
                     WNDVAFHIAIGYPF"
     gene            complement(13081..14163)
                     /gene="proB"
                     /locus_tag="PLD44_03755"
     CDS             complement(13081..14163)
                     /gene="proB"
                     /locus_tag="PLD44_03755"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOF98486.1"
                     /translation="MEFRFKRIAIKIGSNVLTKSDGTLDTESMSNLTNQIATLYHQGI
                     EIVVISSGAVASGRSILGLNKKMDIVDQRQLYSTVGQAKLISLYWDLFAKHHITIGQV
                     LTTKQNFDSRRNYLNQRNCMRVMLENKVLPIVNENDAVSVSELMFTDNDELSGLIASM
                     MGMEALFILSNVDGIYRGSPSDPKAEVIREINRGQDISDFIQTTKSSFGRGGMQTKVS
                     IARKIADQGIEVFIANGRKENILVRLIDAADDVVCTHFIGYRGEVSSVKKWIAHSEDF
                     AKGEIKINTNAVKALMGDKAVSLLPVGVTEIIGDFEKDDIVKIVDENGQSIGVGKVRY
                     DSKKARELIGKKYQKPLVHYDYLYLE"
     gene            complement(14167..15144)
                     /locus_tag="PLD44_03760"
     CDS             complement(14167..15144)
                     /locus_tag="PLD44_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOF98487.1"
                     /translation="MKNFLVFIVMAGFLVSCHSATQKSANEEKVINIPVFDSDSAFSF
                     VQRQVDFGPRVPNTPAHDACANFFVATLKSFGAQVTTQQMDLRTFDGTLLHAVNIIAS
                     YLPESETRILLFAHWDSRPWADNDPNPKNREKPVLGANDGASGTGVLLEIARQLGKNP
                     ANVGVDIVFFDAEDWGASNDDQTSNTENSWCLGAQYWAKHPHKQGYHARYGILLDMVG
                     APQATFYREQYSEYYAAHVVDKVWSQAQNLGFGQYFINQKGGGIMDDHVFVNQIAGIP
                     SINIIHFDPNTSSGFGNYWHTTHDDMSNIDKNTLFAVGTTLMNVIYNEK"
     gene            complement(15298..16005)
                     /locus_tag="PLD44_03765"
     CDS             complement(15298..16005)
                     /locus_tag="PLD44_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5020 family protein"
                     /protein_id="HOF98488.1"
                     /translation="MRKKTTLFFAFLPLAAMAQNFQLHYDFGQGRHYVTTTFEMFKPD
                     DWGNTFFFVDYDFNMDQDHNASLSYMELARCFSLGKTSPFSVQVEYNGGLFAMEGSAY
                     PIQNAFLTGLDYGWHNNNFDRFLNFKVLYKNIVGKHPLSFQLTGVWDLSFYNKRISVC
                     GFADFWREDNLNFTDAAGKNLDSPVLTRYVFISEPQFWYNITPHLSAGSEIEIAANFS
                     SVYGLKICPTLGMKWNF"
     gene            complement(16147..18201)
                     /gene="htpG"
                     /locus_tag="PLD44_03770"
     CDS             complement(16147..18201)
                     /gene="htpG"
                     /locus_tag="PLD44_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOF98489.1"
                     /translation="MTKGTIGVTSENIFPVIKKFMYSDHEIFLRELVSNAVDATQKLK
                     TLASVGEFKGELGDLKIRISVDKKKKTITISDRGIGMTAEEIDQFINQIAFSSAEQFL
                     EKYKNDAQSIIGHFGLGFYSAFMVSKKVEIFTLSYKEGAVAQHWSCNGDPEYVLEDTI
                     KSDRGTDVVLHLDDDNLEFLEEAKIRELLLKYCRFLPVEIAFGKKKEWKDGKYVETDE
                     DEIINNTQPAWIRKPSELTEEDYLNFYRELFPYNEDPLFYIHLNVDYPFHLTGILYFP
                     KIKNNIDIQRNKIQLYCNQVFVTDHVENIVPEYLTLLHGVIDSPDIPLNVSRSFLQND
                     SNVRKISNHITKKVADRLQEIFKNDRNQFEQKWDDLKLFIQYGMLTDEKFYERAEKFA
                     LFKNVDGKYFTYEEYKTLIGENQKDKNGDLIYLYATNAEEQYSYIQHAKDKGYDVLLM
                     DGQLDIHLIGHLEQKWEKTRFVRVDSDVIEKLIQKEDTANVSLSEAQREALTAIFNSQ
                     LPPMEKTNFIITFEQLGPNVNPVLITQNEFMRRMKEMSAMQGGMMNFYGEMPDSYNLV
                     VNTTHPLIEKLLKDEETACAEELAPIEAELLELSNKLQQLKENHKNKKEEEIPAEEKE
                     ELTTLEKRIDELKEKRKSVLAAFASNYKTVRQLIDLALLGNNMLKGEALANFVKRSID
                     LM"
     gene            18772..20595
                     /locus_tag="PLD44_03775"
     CDS             18772..20595
                     /locus_tag="PLD44_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005807855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOF98490.1"
                     /translation="MLQKIIQFFKIYLLILFVFVLQKPFFILYNHQIYSNLSFQDLLL
                     IILHGIKLDASMSAYLTILPGIFLIVSLWAKPSVHEYSQKIYFGLISFIISVVFVVDA
                     ALYPFWGFRLDSTVLFYMKWPKEALASIDIWMIVVGLLAIILIALILYAIFYFLLIKI
                     NKEPKQVKNKLSTACLIFLFTCLLFIPIRGGFNVSTMNVSKVYFSPKNELNHAAINPM
                     FNFLESAFKTQNFDKQYRFMSEEEAQKEFNNLIDQPVQEELPKLFTSPKPNVIFVVVE
                     SFMSNNLEPLGGFPGIAVRMNEFCDEGILFTNFYANSFRTDRGLISIFSGYPAQPTTS
                     IMKYPKKSQSLPSIPKTLKTAGYDLQYYYGGDVDFTNMRAYLIAQGIDKMVVDKDFPL
                     KDRTTKWGAHDHIVFQRLTNDLKKEQKEPFMKILQTLSSHEPFDVPFYKNSDPYLNSV
                     QYTDSCLGAFVNEFRKTKYWENTVLVLVPDHAMHYPKNILLSSVERYQIPLLIIGGAV
                     KSPMKIDTYASQIDIAATLLYQLELPHQDFTFSKNILNPASPHFAFYTFPNGFGFLTP
                     ENHYVFDCSTNKVLWNWGQPNANKKKGEAYLQMLYNDLSKR"
     gene            20800..22587
                     /gene="lepA"
                     /locus_tag="PLD44_03780"
     CDS             20800..22587
                     /gene="lepA"
                     /locus_tag="PLD44_03780"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOF98491.1"
                     /translation="MNNIRNFCIIAHIDHGKSTLADRLLEYTKTVVGKDLQEQVLDNM
                     ELERERGITIKSHAIQMNYQYQSIDYILNLIDTPGHVDFSYEVSRSIAASEGALLVVD
                     ATQGIQAQTISNLYMALDHNLTIIPVINKIDMDSARPEEVEDEIVELLGCSREEIIRA
                     SAKTGQGVEEILNAIVERIPVPQGDPEAPLQCLIFDSIFNPFRGIIAYFKIVNGTLNK
                     GDWVKFVATGKDYYAEEIGVLKLEPQPVQTLSAGNVGYIISGIKTSKEVKVGDTITHV
                     KRPCQKAIAGFEEVKPMVFAGVYPIDTEDFEDLRASIEKLQLNDASLTFEPESSTALG
                     FGFRCGFLGMLHMEIIQERLEREFNINVITTVPNVSYKIYTKKGELIEVHNPSEMPDP
                     TLIDRIEEPYIRASIITPMEYIGPIMTLCLGKRGELIKQEYISGNRIELIYDLPLSEI
                     VFDFYDKLKSISKGYASFDYHMNGYRASKLVKLDILLNGEPVDALSSLIYADNAYSLG
                     RRMCEKLKELIPRQQFEIAIQAAIGAKIIARETIKPVRKDVTAKCYGGDISRKRKLLE
                     KQKKGKKRMKQIGSVEVPQKAFLAVLKLD"
     gene            22726..24273
                     /gene="atpD"
                     /locus_tag="PLD44_03785"
     CDS             22726..24273
                     /gene="atpD"
                     /locus_tag="PLD44_03785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOF98492.1"
                     /translation="MLSIQGKVWQIIGPVVDVFFPLEEKNEFELPPINNALSISLSNG
                     KNLILEVQQHIGENIVRTIALDSTDGLSRGLPVSDLGSPISIPVGNQVKGRLMNVIGE
                     SIDGLSPLGRVESAPIHKEPPKFDELANNDEILYTGIKVVDLLAPYVKGGKIGLFGGA
                     GVGKTVIIMELINNIAKWHKGFSVFAGVGERTREGNDLLREMIEAGVIRYGEEFNKAM
                     QEGKWDLSKVDYEQLKKSQATLVFGQMNEPPGARYSVALSGLTVAESFRDSDESENHD
                     ILLFIDNIFRFTQAGSEVSALLGRLPSAVGYQPTLATEMGLLQERITSTKSGSITSVQ
                     AIYVPADDLTDPAPATTFSHLDATTVLSRQIASLGIFPAVDPLDSTSRILDPLIVGQE
                     HYETAQRVKQILQRNKELQDIIAILGIEELSEEDRLTVQRARKVQRFLSQPFFMAEHF
                     TGVPGVMVPIEETIHGFKLILDGMLDDLPEMAFLNVGTIKDVLNKGHALLREAHNLNK
                     TFKIEDQ"
     gene            24270..24524
                     /gene="atpC"
                     /locus_tag="PLD44_03790"
     CDS             24270..24524
                     /gene="atpC"
                     /locus_tag="PLD44_03790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOF98493.1"
                     /translation="MNLKIVLPNKLIYDGEISSVTVPGKGGVFTILSHHAPLIAILGK
                     GNVTFRFKNGVNNVFINGGLIELNNDKVVICAEQIPENEK"
BASE COUNT         7369 a         4741 c         4982 g         7578 t
ORIGIN      
        1 aggaagcatt tttagtgcaa agtgcgtcga tttcgggatg tcggttcagg taatcatcaa
       61 ggcttgacgc cacaatttct ccctgcgaaa cgatattgat gttgggcgga agaagatttt
      121 taattttatc ctgaagcaaa gtataatgag tgcagcccaa aattaccgta tcaatctgtg
      181 gatcggtttg taacaagtga ttgatatttt tttgaaaaaa ataatcggct ccgggagaat
      241 tgtgttcaaa attttctatg agcggtaccc acatgggaca agcttcggaa gaaagggtaa
      301 tagcatttcc gtacaacttt tgtatttcga gtccgtagga atttgactga acagtgcccg
      361 gagtggccaa taatccaaca tgaccattgt gtgtcagttt atcaattatt tctactgtgg
      421 gtcgaataat tcccagtacg cgcctgcggg gatcaatttt cggcaaatca ttttgctgaa
      481 tggttcttaa tgctttggca gaagctgtgt tacaagccaa aatcaccaaa tgggcaccca
      541 attcgaacag ccgaacgaca cattccagcg tatatttata aacgatttca aaagaacgag
      601 ttccgtaagg agtgcgcgca ttgtcgccca agtaaaggta atcataatgg ggaagccttt
      661 gacggatttt ttccaatatg gtcaagccgc catatcccga atcgaacacg ccgatcggac
      721 cgggcaacga tgataattcc aacttcgtaa gcgaataatt tttgtttcct tgtaattcat
      781 tcattctctg tgaaattttt caaaaaatgg tttattaatc cagaacttct ccgaaattgt
      841 tgctcatttg taccattctg tcgtattctt caggcgacaa atccaggaaa taataatttt
      901 gaggattcat aacctgtccc ttataatgta cttcatagtg gaggtgagga gcagttgatt
      961 tgcccgtact gcctaccagt ccgataatgt ctcctcgttt aacactttga cccggtctca
     1021 ctttgatggt gcttaaatgg gcatatagtg tttcgtaccc aaatccgtga ttaattacta
     1081 tacagtttcc gtaaccctgt tcccagcccg catttacaat agtgccatcg ccggttgcat
     1141 acacatcggt acctgtagga gcaacgaaat ccatcccttc atgaaagcga cgcgtatggt
     1201 aaacaggatc gatgcgccaa ccataacctg atgccattct ttttaaatct ttgttcagca
     1261 caggctgaat ggcaggaaga cattgcaaca tcttttccct gttttttgta aggtttacca
     1321 attcgtcgta agaacgcgat tgaatataaa gctgcttttt cacggtgttc agcattttgg
     1381 ttgtgctgac tacaatttcg gaattcgtca tgtcgtaaag ctggttgtaa taatcggtat
     1441 ttgatgcaat agcttttctt acggtaaacg gaatcggttc ggcttgaagt acaacacgat
     1501 acaagttatt gtctcgctct tccaagtcgg tcaaaacatc ctgtacttca ttgaactggc
     1561 gttgaagcac tttgtattga gcctccattt tgtttttttc agcgtttaat tctctttctt
     1621 caggagattg aactgttgaa gccaagatga agaaaaaaac tgttcctgtt gcaattccgg
     1681 taaaagcgtg aaacaaaaaa cgacgtattt tgaaccacag tgaattttct atcggttcgt
     1741 aacttaaagt atcgggattg aaatgatatt ttttctttgc catagaaaac aatttaggaa
     1801 cataaaaaaa agcgctaaaa attttgaaaa aagaatagat aaatgagaca aattcaactt
     1861 tttatcaagc atgatagtgc aaaaataata aatttggcgt atttttgcag actttatttg
     1921 agattattaa ccttcggtga aatttgcatt cactcaaagg aaaatggatt taattaattt
     1981 cgcaaaatga cttcacagga aattcgacaa tcttttcttg attttttcgc agagaaaggc
     2041 cacaaaattg taccttcggc accaatggtt atcaaagatg atcctacctt gatgtttacc
     2101 aatgcaggta tgaatcagtt taaaaacata attttgggca atgatcccgt aaaatatcca
     2161 cgcgtagcca attcgcaaaa atgtcttcgg gtgagtggaa aacacaacga cttggaagag
     2221 gttgggcacg atacctatca tcataccatg ttcgaaatgc tcggcaactg gtcgtttggc
     2281 gactatttca agaaagaagc catcgaatgg gcatgggaat atctcaccga agtgttgaaa
     2341 atcgataaaa gcagattata tgtaactgtt tttgaaggtt ctccatctga agggctcgaa
     2401 agagatgaag aagctgccgc catttggtcg caatttctgc ctgccgatag aatcatcaat
     2461 ggcagcagaa aagacaattt ttgggaaatg ggagaaacag ggccctgtgg accgtgttcc
     2521 gaaattcaca ttgacattcg gagcgaggaa gaacgcgcaa aacttgacgg gctttcgctt
     2581 atcaatcagg gacatccact ggtgattgag ctttggaata atgttttcat gcaatacaac
     2641 cgaaaagccg atggcacttt ggaagaactt cccgccaaag taatcgatac gggaatggga
     2701 tttgaacggc tgtgcatggt tattcaggga aaaacttcca attacgatac cgatattttt
     2761 caacccatta ttcgcgaaat agggaaaatc agtggtacag aatacggtaa agacgagaaa
     2821 atcgatatcg ccatgcgtgt cgtatccgat catataaggg caattgcctt ttccattgcc
     2881 gatgggcagc tgccttccaa catgaaagcc ggatatgtca ttcgacgcat tctccgcaga
     2941 gccgtgcggt acggatatac ttttctgcat caacacgctc cttttatgta tcggttgatt
     3001 gacgttctgt gcgaaacaat ggggaaagcc tatccagagc tgatcagtca aaagacactg
     3061 atcgaaaaag taataaagga agaagaagaa tcttttttac gtacgcttga aaccggtatc
     3121 cgattgctgg aaagaaaaat ggaagaggct caattggaaa acaaaaaaga aatttccggt
     3181 gccgatgcat ttgttctgta cgatactttt ggatttccca ttgatttaac cgagctcatt
     3241 ttgagggaaa ataattttac cgtcaacatg aacgagtttc atgcagaaat gcaaaagcag
     3301 aaagagcgag cccgaaatgc agccaatatc gaaacgggcg attggattgt tttgcgtgaa
     3361 ggcgacagcc actttgtggg ctacgattgc acttcgtgcg aaacgaacat acttcgttat
     3421 cgtaaaataa aacagaaaaa tcaggaattt tatcagcttg ttttgtctgt tacgccattc
     3481 tatgccgaaa tgggtggtca ggtaggcgac agcggctggt tagtgggtga aaacgaaaaa
     3541 atagaaattc ttgataccaa aaaggaaaat aatcttgcgg ttcatattgc caaacagctt
     3601 ccgtcagaaa ttacgggaac tttcacagcc gttgtacatc ttgaaaaccg acaagccact
     3661 tcctgtaatc attcggcaac tcacttgctg cacgaagctc tgagaacagt tttgggaaca
     3721 catgtggagc aaaaaggttc gtatgttgga ccggacagct tgcgttttga tttttcccac
     3781 tttcaaaaaa tgaccaaaga agaaattcgt gccgttgaag cgcttgccaa caggaaaatc
     3841 cgtgaaaatc ttccacttaa cgaaatgcgg gaagtccctg ttagcgaagc aaaagaaatg
     3901 ggtgcaatgg cattgtttgg cgaaaaatat ggcgaaacgg tgagagtgat tcagtttggc
     3961 agttctaaag aactctgcgg cggaacgcac gtaaaagcca ccggagaaat cggaatggta
     4021 agaataattt ccgaaagttc cattgcggca ggaattcgca gaatagaagc cataacagga
     4081 aaagctgttg aaaaattgat ggatgagcag caggacattc tttcggaaac gagagaaata
     4141 ttgaacaatc ccggtaattt ggtaaacgcc gtcagaaagc tgatggaaga aaatacggaa
     4201 ctgaaaaaac aggtcaacac ctttcaacag gaaaaattgc ttcaacttcg cgaccggctt
     4261 atggctgaag cgaagataac aaatgggatt catgttgttc gctacgaagg aaatttgact
     4321 gctgatcagg ctaaaacgtt ggcattccag attaaaaatc tgcagaacga aaatctcttt
     4381 ttcatcgcag gcaatgtgat ggatggcaaa ccttcgttaa cattgctact ttctgacgat
     4441 ttggtaagca gaggattgaa tgctgctcaa ataattcgag aaattgcaaa ggaaattcaa
     4501 gggggcgggg gaggacagcc tttctttgcc acagccggtg gaaagaatgc tgcagggctt
     4561 tcggctgcca taagtaaagc gctgaaattt attgatgaat ttccgtctaa ataacaaatt
     4621 cattattttt tgaaactttt ttatcaattt gtttgtttta actcaaaatt atttttactt
     4681 ttgcaccgtt aaacaaaaac acttcaagat attttttatg aaaagccgtt tctttacata
     4741 tttttatttt tacttttact ttaaaaggta agagcaggat aatgatatgt aaaaaatggc
     4801 atgaaaaaat gaaattttta aaacaatata gaatcctgct caagtcaatc gagcaggatt
     4861 ttttatttta aggcatataa aatgaaaaga gtagccattc agggtatcag tggagcattt
     4921 cacgaaattg cagcgcgtga gtattttaag ggagaagaaa ttgaggttat tccctgtgag
     4981 acctttaagg atgtttttaa agccatagag gccgataaat cacttttggg aattattgcc
     5041 attgaaaaca ccattgccgg gagtctgttg caaaatcaca atcttttgcg cgaaagcggc
     5101 tgtcttgttg taggagaaca aaagttgcac attgagcatc atttggtggc attgcctcat
     5161 cagaagctcg aagaaattga agaagttcat tcgcatccca ttgctttgat gcaatgtgaa
     5221 gagtttctgg aatcccacaa gcatttgaaa gcggtagaaa gtgaagacac ggctttgtcg
     5281 gctaaagaaa tcgctgatgg tcagatttat aagcgagctg caatttgcag cctttttgcc
     5341 gccgaaaaat atggattgga aattttagag agaaacattg aaaccaacaa acgaaatttt
     5401 acccgtttcc ttattgtagc tgaaccgaca ctggcgttgg aaatgaccaa aaacgatgaa
     5461 atcaacaagg cttctctggt ttttacattg ccgcacgtag agggaagtct ttcgcatgtt
     5521 ctctctattt ttgctttcta tcacatgaat cttaccaaaa ttcaatctct gccgatcatt
     5581 ggaaaagaat gggaatatca attttatatc aacctgacat ttaatgatta ttcgcgatat
     5641 aaacaagcca ttgatgccat tcttccctta atcgacgaat ttcaaataat gggggaatat
     5701 catgcggaaa attaacagct tatttataac tcaactatta taattttaaa acaattaaaa
     5761 atggagattg aacctgccag gcgagtaaat aatgtttcgg aatattattt ctcgaccaaa
     5821 ctgaaagaaa tcgacgagat gaaagccgcc ggtaaagatg tgattaattt gggaatcgga
     5881 agtccggatc ttcctccttc ggaagttact atcgaagcac tttgcaagga agcgcacaag
     5941 ccaaatgttc atggttatca gagttatata ggtattcccg aattgcgcaa cggttttgcc
     6001 gaatggtaca aaaaatggtt tggcgtagag ctgaatccca ataccgaaat tcagccgttg
     6061 ataggctcga aggaaggaat tctacacatt tcgttggcat ttctcaatcc cggtgatggg
     6121 gtactcgttc ccaatcccgg ttatcccacc tatacttccg tcagcaattt gtgcgaagca
     6181 aaagttattc cctacgattt ggacgaaaac catgaatggc agcccgattt tgatgcattg
     6241 gaaaaaatga atctggaagg tgtgaaatta atgtggacca attatcccaa catgcctacg
     6301 ggagccaatg cttctgtgga actttttgaa aaactggtgg cttttggcaa aaagcacaat
     6361 attattattt gtaacgacaa tccctacagt ttcattctta atgatcgaaa gttgagcatt
     6421 ttgtctgttg acggagccaa agatatttgc atcgaaatga attccatgag caagtcgcac
     6481 aacatggccg gttggcgtat tgccatgctg gcttccaatc gccaatttgt ggaatgggta
     6541 ttgagagtta aatcaaatgt cgattcagga atgttccgtc cgatgatgat agccgccgcc
     6601 gaagctttga aaaatccaaa ggaatggcac gatcaaataa atgttgttta tgcacgacgc
     6661 agaaacattg cttttaaaat tctggattta ctcggatgta cttatgatac tcggcaggta
     6721 ggactttttg tttggggtaa aattcctgat cgttacaaag acgccggaga attggccgac
     6781 gaaattttgt atgggaaaaa cgtttttatt acgcccggat ttatttttgg agacaaggga
     6841 aatcgttatg ttcgcatttc tttatgttgt ccggaaccta cactggagga agtggtaaga
     6901 agaatcgaac attgatatgg attttttaga aaaagaaatt gtaaacttat taaaacaagc
     6961 aaaattatga aatttgaatc aattttactg ccgggaatcg aagccaaacg tcccattgtc
     7021 atagcggggc cttgcagtgc cgaaaccgaa gaacaggtta tggaaactgc gcgcgggctg
     7081 gcttctatcg gtataaaaat ttatcgagcc ggtatatgga aacctcgtac caaacccggc
     7141 ggttttgaag gagtaggaga gaaaggtctt ccgtggttga aaagactgaa atcggaattg
     7201 ggattgtaca ccactgtgga agttgccaac gccaaacatg ttcgtgctgc ccttgcacac
     7261 gatattgata ttctttggat tggagctcga actacagcta atccttttgc tgttcaggaa
     7321 attgcagatg cgctggaagg cgtcgatatt ccggtactta ttaaaaatcc ggtgaatccg
     7381 gatttggagt tgtggatagg tgctatagaa agaatttata atgccggaat tcacaaaata
     7441 gccgctgttc atcgcggttt cagttcgtac gacaaaaaaa tttacagaaa tttgcctcaa
     7501 tggcacatcc ccattgaatt gcgccgacaa atgcctgatt tgcctatttt ttgcgatccg
     7561 agtcatattg gcggaaaacg cgaactgatt gctccattat cgcaacaggc tatggattta
     7621 aacttcgatg gattaatgat tgaatcgcat tgcaatcctg aaaaagcttg gagcgatgct
     7681 gcccagcaga ttactccgca ggtgcttgaa tttattttgg aatcactggt tattcgcgac
     7741 accatgcaaa caaccgaaaa tctttctgct ttacgtcgtc agattgatga attggacaac
     7801 aatttgctcg aattgctggc tcaccgcatg cgtgtggctg aagagattgg cagatataaa
     7861 aaggaacaca acatgcctat tcttcaggca cagcgatatg atgaaatttt gcacaatcgc
     7921 gtggagcaag ccattgagat gggaatggat ggtgaattca tgaaaaccgt tcttattgcc
     7981 attcacgaag aatctatccg acatcaaatg gaaattatgg aaaaatcctg aagcttttac
     8041 aaaaaatcag accggcaaat tcgcattttt tgttgctgag ttgccggtct gaaaagttta
     8101 atcgtaagtt gtttttatct tctgcccgaa cgattttcgg agtttgagtt ggtggaacgt
     8161 gtgccccttg aattgttttc agaagttctt tgcgatgtat ttacggtaga attttccgat
     8221 cggcgtaggg tttcggttcg agatgaattt ttttcgtttt gtgaagacgt tctcgtttct
     8281 accgaacgac ctgaatttga aattcttctg gcattgtttt gtgtttcaac ccttccggta
     8341 ttttgacttc ttcttgaaga attgttctcg ctgttgttat agcgtgagtc gctcgaagag
     8401 gtagatattc ttctacttcc attatcctga ttgacaattc tgcgttcctg ttcggttgac
     8461 gatcttctgc tggaattgtt taactcggct ttgttggcaa cattcttgtt cctgctgttg
     8521 aaacctcttt cgggatgtgt ccgctcaaaa tcatttcttc ttaccgtttg gtgaagcctc
     8581 acataagcat ctctgtaata gatattgaca gagttgtgat aatgaaattt gtagtcgcgc
     8641 ggtatgatat ggtgtacatg acccagataa atattaactt ctacaggtct gagcggtcga
     8701 taatgtcttg gataatatcc ccaataatac ggtgaatgcc acggccgata atttttactc
     8761 caaaagaaac tgaaaattac aggttggcga acaaatatcg gctcaataac ataatttaca
     8821 ccaaaaaggt aaggatcacc tattatttgt acatatactt gaggcgagtt tcgcttttca
     8881 accactatgg aagccacatc ctgataagta tatctgtcca acactgcctg aataaccaca
     8941 agatgcaaat tcccgcccga tgtttccacc acgcgcaggt agtcaatcca tccatcgttg
     9001 ttcaagtcaa gatttgaaat ctgataccga gcgtcattca gtctcatttc aaaatccgac
     9061 aagtctcttg attctgcaaa aatggaagca actgcgcgta aatcgagatt atcgcttata
     9121 tcgtaatttc gagcttcaac agtaaaagtt ctttgtgccg gcgtctgggt ggcaaacagt
     9181 aataaagcag ccaaacccaa tattttcgtt ttcataatcg cagctttttt aagtttgata
     9241 attcaaattt ctttactttt taaaaggcac tcatgcatgg tgataaatta aagtcaaaac
     9301 acatgccaaa ttttcaatta tccatatcct attaatacaa ttgacagaat agcaataaat
     9361 taggtgaaaa ttttttattg tttttgaaac tttctttaaa attttttctt caacatgaaa
     9421 acaagaaagc tattttcata aatttgcagt aaaaatatgt aaaggcaaaa gatgaacaca
     9481 ctggatttgg ttattttggt tactgttttt ttcggaatca ttcagggctt gtttagagga
     9541 ttagtaaaag aattgacttc ttttgccgtc attattgtgg gaatatacgg agccaagctg
     9601 ctgagtccgc ttattgcaaa aatgcttgaa agtgttgctt ctttttctcc gcaaatggct
     9661 catccgttgg catttttatt cagtttcatt cttttggcca ttgctatgtt tcttttggct
     9721 cagctgtttg aaaaaacctt gtcaatcatt cagttgggtg gattgaacaa atttcttggt
     9781 gctatagtag gcggattgaa atttttactc gttctaagcg ttttactgaa tgtttttgat
     9841 gcaatggacg aacgttttca ccttataaat aaagccacaa aagaagaatc gttgacttat
     9901 tcccttgttt tgaaagctgg gccggaactt tggaaagaag tgaaggacaa gcaatcgata
     9961 aatgaacaaa atacccatca gcaaaataaa attgtacccc gacaaaacaa atagaaaaca
    10021 acccagcctc attcggagtt tttcttcttt tcaatacgaa tgtggttgcg acgaagccgg
    10081 cagaggatgt ttggcaggtc cggttgtggc tgcagcagtc attttgcctc cggattttat
    10141 ttgtcctcaa ttgaacgatt cgaagcaatt gagcccgttg cttagagaaa aattcagaaa
    10201 aataatagaa aaggaagcac ttgcatggtc ggttgcctcg gtcgataatc gagaaataga
    10261 cagaattaac atattgaatg cctctatttt agccatgcac aaggccatcg acggtttgca
    10321 gatcaaacct caatttattg ctgttgacgg aaaccggttt aagccttatg ccgatattcc
    10381 gtttcaaacc attgtaaaag gagacagcaa gtatctttcc attgccgcag catcggtatt
    10441 ggccaaaacg cacagagatg aactgatgga aatactcggg gagaaatttc ctgaatatga
    10501 atggaagaaa aacaagggat atcctacagc agagcatcgt gaaatgattc gtcggtatgg
    10561 ctgcacggag tatcaccgat taagttttcg tctgctgaag gaggaaccat tttaaccaaa
    10621 cagaactgca accggaaaaa tgctcgtcag aaaggataac caatggcaat gtggaaagcc
    10681 acgtcgttcc attgcggcga aaccctccat cggtcgctgc gtttcagtac cgggtcgtaa
    10741 agttttactc ccatatccag acgggcaacg ataaaggaaa aatcgaatct tagtccgact
    10801 ccgtaagcaa ctgcaatttg attcataaag gaatcccatt tgaacaatcc accctgttgc
    10861 gtatcataat ccttgatagt ccatatattt cctgcatcca taaaaaccgc actttcgagc
    10921 aaccaaaaca attttccgcg atattccata ttcatgtcga gtttgatgtc gccggtctga
    10981 ttgaaatctc tgcgttgggt attgattctt ttataaacac ccggccccaa tgtgctttca
    11041 ctccaacctc tgacgccatt ggctcctccg ctgaaaaatc gtttttcgta cggaactgca
    11101 tcggcatttc cgtaaggaat agctattcct aatccggcat ggtaaacaaa acgattgttt
    11161 tcatcgaaaa tttgatgata agttagatta tattctgttt tgaaatactg agcatacgca
    11221 atgttgagca ggcggtaatt tccatcttcc gccggacggc tgcccagcaa atgatccagt
    11281 ccgtatagaa tatttccggc cgtttctatg ctgtaacgca tggtacggta gttccgtaac
    11341 ggttgattgg cgttaaagtt tgtggaagag cctgaataac ccattcgcat gataaagtga
    11401 ttttcgtaat tgtaagaatt aaaaatgccg gttgtgagaa aactgtcgcg gaaagcaggg
    11461 aaaatttcgg gaaaatatac atagttgact tcaaacaaat caaaaatgtg ggtatattgt
    11521 cttctgttga gaaaatatct catgccggca cccaagttgc ttgttcgaaa ttcgcccggt
    11581 cgaaactgat aatagtatga accggaaaat tccgtgttag cccgaaggtt tctcttgaaa
    11641 tcataagaag caaatggtaa aaccacacga ggaaattgca gcccaatctg ccctccccat
    11701 tcctgtgccc acaccttttc ctgaagttca taagctccat gaacctgcag ggataaagtt
    11761 tcggcacctt taaaaatatt tcgattgaca aggttcaaat tggttgctcc accccagtat
    11821 ccatccgtat aagtggcttc gacttcagca gaaagagaaa tggctttccc cggaataatg
    11881 ctgatgtggc aatcgagtag acccgtatcg acctgttcaa aaccgatatt gacaaacttt
    11941 atggcaccca gcgaattaag tcctgaataa gttttttcta tgttggcatc gttaaataaa
    12001 gcttgtggat caatatatgt gttctgaacc aatgcgtcaa ttttaataat ttgcttgtgt
    12061 ggagttatca tggcaaaatt tcggaaccac accgtatcct tttccatagg ttgattcagt
    12121 gatggaaggt cagtggggtt aatttcgaaa tacacgtttc tcacataaaa ttttctgaat
    12181 atgatttggg aaagtgagtc gtttttgtcg ttcagataat ctctcaattg catggacaaa
    12241 tccacctgat gcgttttttc tatgctgtcg gccgaataaa ctataaaatc cttactgaaa
    12301 ttgtaatagc ctgtttggcg aaaacgagaa gtgattcttt cccgttcggc atcgagcata
    12361 tccacgtcaa aaagcattcc cggccgaatg agcgagcggg aagtgtcggc tgcaatagct
    12421 tgcagttcgg gatggttcaa ctgtaccgaa taattccgga tacggtaggg ttgatgagca
    12481 agaatttggt attcaacttc ggctttttta tccttaagtg taactttgtt ttccactttc
    12541 gctctcaaaa aacctttatt gtgcatgaaa agctgaagtt gctgtgagga aatttccgtc
    12601 agggcaggat tataaattac cggttcttct ccaatgcgca tgagtgtttt gttgatccac
    12661 tttgaagtgt cttttcctgc aagactgtaa atgccaagtt gggtacggaa aagaccaaaa
    12721 accgaagaat tgggtgtttg acgcaaatat tcttttaatt cgattttttt gacattccgc
    12781 gtgtctatgt ttatgttcac tttgtccaac aaatattctc cttccggaac gaattttgtt
    12841 gtgttacatc ccgttagcag taaagccact gcaatcgttt caattttaag tcttattttt
    12901 atgtttcgca tagacgctgc cttgcttttt tctctcacaa aaccaattta atccttcacc
    12961 attcaaaatc tgtgcgcaaa attgtaactc atgcaaaaaa tgaatgagat acgggtaaaa
    13021 aattaattta ataaactgga tgtcaaataa tcaacatttt tagaaacttt tttcatcaat
    13081 ttattccaga taaagataat catagtgtac caaaggtttc tgatactttt ttcctattaa
    13141 ttctcttgcc tttttgctgt cgtagcgaac ttttccaact ccaatcgact gcccgttttc
    13201 atcaacaatt ttcacaatat cgtctttttc gaaatctcca ataatttccg ttacaccgac
    13261 aggcagcaaa ctcacggctt tatcgcccat cagcgctttt acggcattgg tatttatttt
    13321 tatttcgcct ttggcaaaat cttcactatg agcaatccat ttttttacgc tcgatacttc
    13381 gcccctgtat ccgataaaat gtgtacaaac cacatcgtca gcggcatcta tcaagcgaac
    13441 caaaatgttt tcctttctgc cgttggcaat aaatacttca atgccctgat cggctatttt
    13501 tcgggcaatg gaaactttgg tctgcattcc tccacgtccg aaacttgatt tggtggtctg
    13561 aataaaatcg gaaatatcct gtcctctatt tatttcccga ataacttctg cttttgggtc
    13621 ggaaggagaa ccacgataaa tgccatccac gttgctgaga atgaaaagtg cttccatccc
    13681 catcatggag gctattaaac ccgaaagttc gtcgttatcg gtaaacatca attcagaaac
    13741 agaaactgca tcgttttcgt ttacaatagg taaaaccttg ttctcgagca ttacgcgcat
    13801 gcagttgcgt tgattcagat aatttcttcg gctgtcgaaa ttctgtttgg tggtaagcac
    13861 ttgtccaatg gtaatatggt gcttggcaaa caaatcccaa tacaagctga tgagttttgc
    13921 ctggcctaca gtggaatata attgacgctg gtcaacgata tccatttttt tgttcagtcc
    13981 caggatgctt cgacccgaag caactgctcc ggaagaaatt acaactattt ctattccttg
    14041 atgatacagt gtggcaatct gatttgtcaa attcgacata ctttcggtat cgagcgtacc
    14101 atcagatttg gtaaggacat tacttccaat ttttatcgca attcttttaa aacgaaattc
    14161 catatattat ttttcgttgt aaattacatt catcagggta gttcctacgg caaaaagggt
    14221 atttttatca atattactca tgtcgtcatg cgtagtatgc cagtaatttc cgaaccccga
    14281 ggaagtattg ggatcaaaat ggataatatt gatgctcggg attcctgcaa tttgattcac
    14341 aaaaacatga tcatccatga ttcctccacc tttctggttg ataaaatact gaccaaaacc
    14401 gagattttgc gcttgagacc aaactttgtc cacaacatga gcagcataat attccgaata
    14461 ttgttcccga taaaaagttg cctgtggtgc tccaaccata tccagcaaaa tgccgtatcg
    14521 tgcatgataa ccttgcttgt gaggatgttt tgcccaatac tgagcgccca agcaccacga
    14581 gttttccgta ttggaagttt ggtcatcatt ggatgctccc cagtcttcgg catcaaaaaa
    14641 aactatatcg actcccacat ttgccgggtt tttccccaat tgtcgagcta tttcgagtaa
    14701 aacacccgtt ccgcttgctc catcatttgc tcccaaaacg ggtttttccc gattttttgg
    14761 atttgggtcg ttgtcggccc aggggcgcga gtcccagtgt gcaaaaagca aaatccttgt
    14821 ttccgattcc ggtagatagg aagcaataat gttcacagca tgaagcaaag ttccatcaaa
    14881 agtccgcaag tccatttgtt gagttgtaac ttgtgcccca aaacttttca gtgttgcaac
    14941 aaaaaaattg gcacaagcat cgtgtgcggg cgtatttgga acacggggtc caaaatcgac
    15001 ctgacgctga acaaacgaaa aagccgaatc tgagtcaaac acaggaatat ttatgacttt
    15061 ttcttcatta gcagattttt gggttgccga atgacaggac accaaaaatc cggccattac
    15121 aataaatacc aaaaaatttt tcactgaatg ctgtttttct gcgataaaaa tttgctgctc
    15181 atttttgttc aacagtttca ttttctattt aaaatgagat tacaatcagc aaagataatg
    15241 aattgaaatg aatttttcgg tatgcttttt tctaaaatgt ttcaggggaa attcatttta
    15301 gaaattccat ttcattccca atgtaggaca aatttttaat ccataaacgg aggaaaagtt
    15361 ggctgctatt tctatttccg aaccggcaga aagatgtggg gtaatgttgt accagaattg
    15421 tggttcggaa ataaagacgt accgagtaag caccggtgaa tcgagatttt taccggcggc
    15481 atctgtgaaa ttgagattat cttcccgcca aaagtcggcg aaaccacaaa cggaaattct
    15541 tttattgtaa aagcttaaat cccaaactcc cgtaagttgg aaagagagag gatgtttgcc
    15601 aacaatattt ttgtaaagca ctttaaaatt cagaaaacga tcgaaattgt tgttgtgcca
    15661 gccatagtcg agtccggtca gaaaagcgtt ttgaatggga taagcagatc cttccatagc
    15721 aaacaaaccg ccgttgtact ccacctgaac actgaaagga gacgttttac ccaatgaaaa
    15781 acatcgtgcc aattccatat aagaaaggct tgcattatga tcttgatcca tgttgaaatc
    15841 gtaatccaca aaaaagaaag tgtttcccca atcatcgggt ttgaacattt cgaaagtcgt
    15901 tgtaacgtaa tgccgtccct gtccgaaatc gtaatgcagt tgaaaattct gagccatggc
    15961 agccagcggt aaaaaggcga aaaataaagt cgttttcttt ctcattattt tagtaatcag
    16021 ttagttacat aaaatgaagt tgcaaaagta atacaaaaaa cagcatgttc aaaatatcta
    16081 cacaaaaaaa gcccgtcagt ttaggaaaat ccgacgagct tcaacaagta aagcagaaag
    16141 aaatttttac attaaatcta tgcttcgttt cacgaaattg gcaagagctt ctcctttcaa
    16201 catgttgttg cccagaagtg ccagatcgat caactgacgc actgttttat agttgcttgc
    16261 aaaggctgca agaaccgatt ttcttttttc tttcagttca tcaatccttt tttccaacgt
    16321 tgtcaactcc tctttttctt ctgccggaat ttcttcctct tttttgtttt tatgattctc
    16381 tttcagttgt tggagtttgt tgctcaattc caaaagttcg gcttcaatcg gtgccaattc
    16441 ttcggcacaa gccgtttctt catccttcag caatttttcg atcaacggat gggtagtatt
    16501 taccaccaga ttatagctat cgggcatttc tccgtagaaa ttcatcattc cgccttgcat
    16561 ggccgacatt tccttcattc gacgcatgaa ttcgttttga gttatcagta ccggatttac
    16621 attgggtccc agttgttcaa aagtaatgat aaagtttgtt ttttccattg gaggcaactg
    16681 agaattgaaa attgcagtca atgcttcgcg ttgtgcttcg cttaatgaaa cgttggcggt
    16741 atcctctttc tgaatgagtt tttctatcac atcactgtca acccgtacaa aacgcgtttt
    16801 ttcccatttc tgctccaaat gacctatgag atgaatatcc agttgaccat ccataagcag
    16861 aacatcatag cctttgtctt tggcgtgttg tatgtaactg tattgttctt cggcgttggt
    16921 tgcatacaga taaatcaaat ctccattttt gtctttctga ttttcgccaa tcagggtttt
    16981 gtattcttcg taagtgaaat atttgccatc aacatttttg aacaaggcaa atttttcggc
    17041 tcgttcataa aatttttcgt cggtcaacat gccatactga ataaacagtt tcaggtcgtc
    17101 ccatttctgt tcgaactgat ttctgtcatt cttaaagatt tcctgcaagc gatcggccac
    17161 ttttttggta atatgattgg aaattttccg aacattcgag tcgttttgca aaaaacttct
    17221 ggatacattc aaaggaatat ctggagaatc gataacgcca tgcagcagag ttaaatattc
    17281 aggaacgata ttttcaacat gatcggttac aaatacctga ttgcagtaaa gttgaatttt
    17341 gtttcgctgt atgtcgatat tgttttttat tttagggaaa tacaggattc cggtcaggtg
    17401 gaaaggataa tccacattca aatgaatata gaacagcgga tcttcgttat agggaaacaa
    17461 ttcacggtaa aaattcagat aatcttcttc cgtcagttca cttggtttgc gaatccatgc
    17521 aggctgcgta ttgttgataa tctcatcttc atcagtttcc acatatttgc cgtctttcca
    17581 ttccttcttt ttcccaaagg caatttcgac cgggagaaaa cgacagtatt tcaaaagcaa
    17641 ttcacgaatt ttcgcttctt ccaaaaattc aagattatcg tcatccaaat gtaaaacgac
    17701 atccgtccct ctgtccgatt taatggtatc ttcaagtaca tattccgggt ctccgttgca
    17761 cgaccagtgt tgggcaaccg caccttcctt gtacgacagg gtgaatattt ccactttttt
    17821 tgaaaccata aatgcagagt agaaaccaag tccgaaatga ccaataatgg attgtgcatc
    17881 gttcttgtac ttttcgagaa attgctcggc actggaaaaa gcaatttgat tgataaactg
    17941 gtcaatttct tcggcggtca tgcctattcc gcggtcgctg atggtgatgg tttttttctt
    18001 tttgtcaaca gaaatgcgga ttttcaaatc tcccaattct cctttgaatt cacccactga
    18061 agctaaagtt ttaagctttt gggtagcatc tactgcattg gaaaccaatt cgcgaagaaa
    18121 aatttcatga tcgctgtaca taaatttctt aatcacgggg aaaatatttt cactcgtaac
    18181 ccctatagtc ccttttgtca tgtttatttt ggatttaata agtttttaaa ttccttttat
    18241 gcgttggaaa agcgaaaaaa tctctgattc ccggcttttc ccgccgattt tttgcaaaaa
    18301 aaatgccaat gctttttccg gtgacatatt gacagccata ttgaaataaa ttttatcgca
    18361 ttttaaattg gtaattttta ttacgaataa aaaagccttt atactcggct cataactgat
    18421 ttaaaggaag agtctcctct gaaaagttac tcaaagagaa ttggtttttt taagacattg
    18481 agataaaaag gaagaaaaat atttaaaaaa gttttttgac aattttcatc agaatataat
    18541 ttatttttta aaggtctgat gtaacccaca tgtctgaata ttatattcat tcgtgtttgt
    18601 gtaagtaata catgtgggtt tcatatatta tcgagtttaa aatataattc aaaaaaaatt
    18661 ttcagaaaat ctattctttt gaaaggaatc gtttaacttt gtccgatttt tcttaaaatc
    18721 agggttgaaa gtaatttttt ccaaatttcc aactttttca aaaaacttgt aatgctgcaa
    18781 aaaataattc aattttttaa aatttatttg ttgatacttt ttgtttttgt gcttcaaaaa
    18841 ccatttttca ttctttataa tcatcaaatt tacagtaatc taagttttca ggatttatta
    18901 ctaattattc tacacggaat aaagctggat gcgtccatgt cagcttatct cactatttta
    18961 ccgggaattt tcttaattgt ttcgttatgg gcaaaacctt cggttcacga atactctcaa
    19021 aaaatatatt ttggtcttat ttcgtttatc atttccgttg tttttgtggt tgacgctgca
    19081 ctgtatccat tttggggttt tcgtctcgat tccactgtac tgttttacat gaaatggccg
    19141 aaagaagcat tggcaagcat cgatatttgg atgattgttg tcgggctatt ggctatcatt
    19201 ctgattgctt taattttgta cgcaattttt tactttttgc ttatcaaaat caacaaagaa
    19261 cccaaacagg tcaaaaacaa actttctaca gcctgtttga tatttttgtt cacctgtttg
    19321 ctgtttattc ctatcagagg cgggtttaac gtttccacca tgaatgtgag taaggtttat
    19381 ttcagcccga aaaatgagtt gaatcatgct gccattaatc ccatgttcaa ttttttggaa
    19441 tcggcattca aaacacaaaa ttttgacaaa caataccgct tcatgtctga agaagaggct
    19501 caaaaagaat ttaataattt aatcgaccag ccggtacagg aagaactacc taaattattt
    19561 accagtccga aaccaaatgt tatttttgta gtggtggaaa gtttcatgtc caacaacctt
    19621 gagccgctgg gaggttttcc cggaattgcg gtacgcatga atgaattttg cgatgaaggc
    19681 atcctgttta ccaactttta cgcaaacagt tttcgtaccg atcgcggact gatttccatt
    19741 ttcagcggct atccggctca gcccacaact tccattatga aatatcccaa gaaaagccag
    19801 tctttgcctt ccattcccaa aactttgaaa actgcaggtt acgatttgca atactactat
    19861 ggaggcgatg tggacttcac caacatgaga gcttacctga ttgctcaagg catcgacaaa
    19921 atggttgttg acaaagattt tccgctcaaa gacagaacaa cgaaatgggg tgcgcacgat
    19981 cacatcgttt ttcagcgttt aaccaatgat ttgaaaaaag aacaaaagga accctttatg
    20041 aaaattcttc agacactcag cagccacgag cctttcgatg tcccctttta taaaaacagc
    20101 gatccctatt taaattcggt tcaatatacc gatagttgtt tgggcgcatt tgttaacgaa
    20161 ttcagaaaaa ccaaatactg ggaaaatact gttttggttc ttgtgccgga ccatgccatg
    20221 cattatccaa aaaatattct actttcttcg gttgaaaggt atcaaattcc actgctcatt
    20281 attggcggtg ctgtaaaatc gccaatgaaa atagatacgt atgcgtccca aatagatatt
    20341 gctgcaaccc tactttatca acttgaatta ccgcatcagg attttacgtt cagcaaaaat
    20401 atcctgaatc cggcttctcc ccattttgct ttttacacgt tccctaatgg tttcggtttt
    20461 ttaacccctg aaaatcatta cgtctttgac tgcagtacaa acaaagtgct ttggaattgg
    20521 ggacaaccga atgccaacaa gaaaaaaggt gaagcctatc ttcaaatgct ttacaacgat
    20581 ttatcgaaac gttaattaaa aaggtttaat ataactaaag cattgatttt caatttatta
    20641 taagaatgta cgccattcga taatttgatt tatatcatta gattaatcta tttcttctaa
    20701 tacaaatcaa acaattctac aaatctattt ttatccgttt gtatgttaat tttgaatttt
    20761 catgtatttt tgtagttctt aaaatttgaa gaaaatcgaa tgaacaacat ccgaaacttt
    20821 tgtattattg ctcatattga tcatgggaaa agtacgcttg ccgaccgttt attggaatat
    20881 accaaaacgg ttgtgggaaa agacttgcag gaacaggtgc tcgacaatat ggagctggaa
    20941 cgtgaacgtg gcatcactat aaaaagccat gctatccaaa tgaattatca gtatcaaagc
    21001 attgattata ttctgaattt aattgatact ccgggacatg tagatttttc gtatgaagtt
    21061 tcgcgttcca tagctgcttc ggaaggagct ttgctagtag tggatgcaac gcagggcatt
    21121 caggcgcaaa ctatttcgaa cctctacatg gctcttgatc ataatttgac cattattccg
    21181 gtgatcaaca aaatagatat ggacagcgca agaccggaag aagtggaaga tgaaatagta
    21241 gaattgctcg gttgtagccg ggaagaaatt attcgagcta gtgcaaaaac gggacaagga
    21301 gtcgaagaaa ttcttaatgc tattgtagaa cgcatccctg taccacaagg agaccctgaa
    21361 gcgccattgc aatgtttgat tttcgattcc atttttaatc ctttcagggg aattattgcc
    21421 tactttaaaa ttgtaaatgg aacactcaac aaaggagatt gggtaaaatt tgttgctact
    21481 ggcaaagatt attatgccga agaaattgga gtgctgaagc tcgaacctca acctgtacaa
    21541 actttgagcg cgggaaatgt gggctatatt atttccggaa tcaaaacatc caaagaagta
    21601 aaggtaggtg atacgataac acatgtgaaa cgtccctgtc aaaaagcaat tgccggtttt
    21661 gaagaagtta agcctatggt atttgccggt gtttatccga ttgatacaga ggattttgaa
    21721 gacttacggg cttccataga aaaattgcag ttaaacgatg cttcgctcac tttcgagccg
    21781 gaatcttcca cagctttagg attcggattc cgttgcggat ttttgggaat gttgcacatg
    21841 gaaattattc aggaacgtct ggaacgtgaa ttcaacatta atgttattac caccgttcca
    21901 aatgtttcct ataaaattta taccaaaaag ggagaactta ttgaggtgca caatccttcc
    21961 gaaatgcctg atccgacttt gatagatcgc attgaggagc cttatatcag agcttccatt
    22021 atcactccga tggaatatat tggtcccatt atgactttat gtttgggtaa gcgtggagaa
    22081 ctgatcaagc aggaatacat ttcgggcaat cggattgaat tgatttacga cctgccgctg
    22141 agcgaaatag tttttgattt ttacgacaag ttgaaaagca tttcaaaagg ttatgcttct
    22201 ttcgactatc acatgaatgg ctacagagct tccaaactgg taaagctgga tattttgctc
    22261 aacggagagc ctgtcgacgc actttcttca ttgatttatg ccgataatgc ctattcattg
    22321 gggcggcgaa tgtgcgaaaa attgaaggaa ctgattcccc gtcagcaatt tgaaattgcc
    22381 atccaggcag ctatcggggc caaaattatt gcgcgagaaa ccattaaacc cgtcagaaaa
    22441 gatgttactg ccaaatgtta tggcggcgat atttcacgaa agcgaaaact gctggaaaaa
    22501 cagaaaaaag gaaaaaaacg aatgaagcaa atcggatcgg ttgaagtacc acaaaaagcc
    22561 tttttagctg ttttgaagtt ggattgaaaa actgaataat attgtttaac agaaagattt
    22621 gtttttttga actaatttaa acattcgttt tcttttaaga agaatctttt atttactttt
    22681 gccacacaat ttctcacaaa atgtatttac aattataatt ttttcatgtt atcaatacag
    22741 ggaaaggtgt ggcagataat tggtccggta gtggatgttt ttttccctct cgaagagaaa
    22801 aatgaatttg aattgcctcc tataaataac gctttgagta tttcgctttc caacgggaaa
    22861 aatctcattt tagaagttca acaacatatt ggcgagaata ttgtccgaac aattgcgctg
    22921 gattccactg atgggttaag tcgaggtctg cccgtaagcg atttgggttc gccaatttcc
    22981 ataccagtag ggaatcaagt aaagggaaga ttgatgaatg tcatagggga atcgatagat
    23041 ggcttaagcc cactgggaag ggtagagtcg gctcccatac acaaagaacc ccccaaattt
    23101 gatgaacttg caaacaacga cgaaatttta tataccggaa ttaaagttgt cgatttactt
    23161 gctccttacg ttaaaggtgg aaaaattgga ctttttggcg gcgccggtgt tggcaaaacg
    23221 gttatcatta tggagcttat taacaatata gccaaatggc acaaaggatt ttcggttttt
    23281 gccggcgttg gtgaacgaac ccgtgaaggg aatgacctgt tgcgtgaaat gatagaggca
    23341 ggagttattc gctatggtga agaatttaac aaagctatgc aagaagggaa atgggattta
    23401 tcgaaagttg attatgagca actaaaaaaa tctcaggcga ctttggtctt tggacaaatg
    23461 aacgaacctc ccggagcacg atattccgtt gccttgtcgg ggcttacggt agctgaatct
    23521 tttcgcgaca gtgatgaaag tgagaatcat gatattttat tgtttattga taatattttc
    23581 cgtttcactc aggcaggctc tgaagtttcg gctctgttgg gtcgattgcc ttctgctgtg
    23641 ggttatcaac ctacacttgc tactgaaatg gggcttcttc aggaacgcat tacttccact
    23701 aaaagtgggt caattacttc tgttcaagct atttatgttc cagcggacga tttaaccgat
    23761 cctgctccgg caactacttt ttcacatttg gatgctacaa cggttctcag tcggcaaatt
    23821 gcttcattgg gaatttttcc tgctgtcgat ccactggatt ctacttcacg aattctcgat
    23881 ccgcttattg tcgggcagga acattatgaa acagctcagc gggtgaagca aattcttcaa
    23941 cgaaacaagg agttgcaaga tattatcgcc attctgggta tagaagaact ctctgaagaa
    24001 gatcgtttaa cagtgcaaag agctcgaaaa gtacaacgtt ttctttcgca gcctttcttt
    24061 atggcagaac attttacagg agttccggga gtaatggtac ctatagaaga aaccattcac
    24121 ggctttaaac ttatattgga cggtatgctc gatgaccttc ccgaaatggc ttttcttaat
    24181 gttggtacca ttaaggatgt cttaaataag gggcatgcat tgctaagaga agctcacaat
    24241 ttgaataaaa catttaaaat tgaggatcaa tgaatctgaa aattgttttg cccaataaat
    24301 tgatttacga tggtgaaatt tcatctgtaa cagtaccggg taaggggggg gtatttacca
    24361 tactttctca tcacgctcct ttgattgcca ttttggggaa aggaaatgtt acttttcgtt
    24421 tcaaaaacgg tgttaacaat gtttttatta atggcggact aatcgaattg aataacgata
    24481 aggtcgttat ttgtgctgaa caaattccag aaaatgaaaa ataatcttct tttttcatta
    24541 gttttttttt ctttactaac attgttgtta gggctatttt tctggttgct cttgaaaaaa
    24601 ttttcttcca cgactatcga aaattatttt ttgttgcctt tattgtttta tttttctgga
    24661 ttgactttaa
//
LOCUS       DAOSIB010000025        24432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_2286_length_24432_cov_11.691430, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000025 DAOSIB010000000
VERSION     DAOSIB010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 24432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24432
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2286_length_24432_cov_11.691430"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(598..672)
                     /locus_tag="PLD44_03795"
     tRNA            complement(598..672)
                     /locus_tag="PLD44_03795"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(636..638),aa:His,seq:gtg)
     gene            784..1464
                     /locus_tag="PLD44_03800"
     CDS             784..1464
                     /locus_tag="PLD44_03800"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOF98494.1"
                     /translation="MNKQTIEQNNEVKEILQKLWLSRNGEVASQMRQYGMVYRKNLGV
                     AITRIREIASDYEPNEETAQQLWTLHGRETMLMAILLQPVNNFSWEMAQSWAEDCDNI
                     ETIEFACMHLFSKLPFVNELCCDWCASDKVQLQIMGFILSARNYQNFSDVDRNLIIEK
                     AFENSTTDNFHLYKAISLALCRFCRNGKSIADFILEKMSNFLPQPTAGQQYIMEEVKS
                     EMNFLELL"
     gene            1498..1971
                     /locus_tag="PLD44_03805"
     CDS             1498..1971
                     /locus_tag="PLD44_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOF98495.1"
                     /translation="MDENKEKEENSFEVVKKIFNDYLAAHQHRKTPERYAILEQIYTH
                     EGHFNAELLYNAMQKDYRVSLATIYNTLDLLLECKLIVKHQFGDQLAQYEKTFGTNIH
                     HHLICTQCGKIKEFSDKNIKAAIQSKHFQTFQMSHYSLYIYGICSKCLKKKKKTS"
     gene            2096..3364
                     /locus_tag="PLD44_03810"
     CDS             2096..3364
                     /locus_tag="PLD44_03810"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOF98496.1"
                     /translation="MKADVLLGLQWGDEGKGKVVDVLTPHYDIVARFQGGPNAGHTLE
                     FKGKKFVLRSVPSGIFQGDKINVIGNGMVIDPALFKEEVEALEAAGEPLHQRLKISKK
                     AHLILPTHRLLDLASEAAKGTGKIGTTGKGIGPAYTDKISRNGLRIGDILHRFDEKYR
                     EVVARHTEMLRQYNFEYDLAALEKTFFEGIECIKRFDLIDSEYFINHSLEEGKKILAE
                     GAQGTLLDIDFGSYPFVTSSNTICAGACTGLGLSPQKIGEVYGIFKAYCTRVGSGPFP
                     TELKDETGELIRKKGCEFGAVTGRPRRCGWIDLVALRYSIMLNGVTQLIMMKSDVLDD
                     FDTIKACVAYRIDGKETEEFPFEITENLEPVYVELPGWKTKISNLQSEDEFPEELNAY
                     LNFLEEELNVPIKIVSVGPDREQTVIRYQE"
     gene            3465..4142
                     /locus_tag="PLD44_03815"
     CDS             3465..4142
                     /locus_tag="PLD44_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOF98497.1"
                     /translation="MIYIIFIVFALISFLLQNRLQSKFKRYSKLPVPNGMTGKDVAEK
                     MLHDNGIYDVKVISTSGHLTDHYNPANKTINLSEQVYASSNVAAAAIAAHETGHAVQH
                     AYAYAPLTLRSKLVPVVSFASMWMQWVLLAGIIFLQSFPQLMLTGIILFALTTLFSFI
                     TLPVEINASQRALAWLNNAGITNYETHEPAKDALKTAAYTYVVAALGSLATLVYYILI
                     FMGGSRR"
     gene            4250..4323
                     /locus_tag="PLD44_03820"
     tRNA            4250..4323
                     /locus_tag="PLD44_03820"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4284..4286,aa:Arg,seq:ccg)
     gene            4505..5911
                     /gene="rlmD"
                     /locus_tag="PLD44_03825"
     CDS             4505..5911
                     /gene="rlmD"
                     /locus_tag="PLD44_03825"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOF98498.1"
                     /translation="MRKKKQLPVLEKVTITDIGAEGKALTKINDMVVFVPLVVPGDVV
                     DLQVTQKKKNYMEARAVKFHHYSEQRCEPFCQHFGICGGCKWQMLPYAEQLKYKQKQV
                     EDHLKHIGKVQLPEISPILPSEKTVFYRNKLEFTFSNRRWRSFEEISSAQNIEDENAL
                     GFHIPGHFDKVLNIEKCWLQDDISNKIRNEIRRYALENELEFFNLRRQEGFLRTMMVR
                     TSTTGEVMLLVVFFREDETKRKALLQHIADTFPEITSLLYVINSKANDTITDQEIISF
                     TGNDSIYEKMEDLTFKIGAKSFFQTNSAQACRLYEKARDFAALTGNELVYDLYTGTGT
                     IANFVARKAKKVIGIEYVPEAVEDAVNNSKLNNITNTFFYAGDLKEVMTANFIREQGN
                     PDVIITDPPRAGMHGDVVDAILTTNAARIVYISCNSATQARDLQLLDSKYKVTAVQPV
                     DMFPHTHHVENVVLLEHR"
     gene            6133..7593
                     /locus_tag="PLD44_03830"
     CDS             6133..7593
                     /locus_tag="PLD44_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOF98499.1"
                     /translation="MISFFISIGLLIAGYFIYGKITERIFGIEPNRPTPALANPDGVD
                     YIPLPWWRVFLIQFLNIAGLGPIFGAIMGIMFGESAFLWIVFGTIFAGGVHDYLSGMM
                     SIRNNGTSLPELVGNELGNGIKQFMRVFSLALMILVGAVFVSGPAQLLANFTPHSINV
                     SGWIVIVFTYYILATLLPIDKIIAKIYPFFGFVLLFMAVGIFLALILNKAPIPEFTEG
                     FANLHPNKLPIFPIMFVSIACGAISGFHATQSPLMARCIQNEKLGRRIFYGSMVAEGI
                     VALIWAAAASSFFGSIQGLQEFLGTLPATANKPAVVVDRICRDWLGNIGGVITLLGVI
                     AAPLTSGDTALRSARLIVADFIHLEQKSWKKRLMISIPIFILTFIVLQINFDALWRYF
                     AWCNQTLAVFTLWSITVYLAKRHKGYWISLIPAVFMTCVSVSYICIAPEGFQLPQYLS
                     YLISAIACVVVIFFFYRFIYLNKEKLLTENQLFNKK"
     gene            7779..8909
                     /locus_tag="PLD44_03835"
     CDS             7779..8909
                     /locus_tag="PLD44_03835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF98500.1"
                     /translation="MAITVREVTTKKQLKQFVWFGINLYKDNEYAAPPLFIDDYTNLT
                     KGKNPSLDFCEAAYFLAYNEKNEIVGRIAAIINPVANETWNQKNARFGWVDFIDDYEV
                     SKALFDAAVEWARAKGMTAMQGPLGFTDFDHEGTLIEGFDRLGTLAGIYNYPYYSQHI
                     EKYGFVKDADWVEFLITIPEKFPDKYFRIAELVKQKYHLTPRHFKSRKKLVNGYGNKI
                     FDLLNTCYQHLYGFTKLNEAQKQFYIKLYFSFFRLDTVSVVTDENDDVVAVGIAMPSL
                     TKALQKAKGKIFPLGWFYLLKSIYYKNDIVDLYLIAVHPRYQGKGVNSILFAELMVNF
                     AKNGYKFAESNPELETNTQVSSQWSDFEHINHKRRRAYIKQI"
     gene            9074..10660
                     /gene="nadB"
                     /locus_tag="PLD44_03840"
     CDS             9074..10660
                     /gene="nadB"
                     /locus_tag="PLD44_03840"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOF98501.1"
                     /translation="MTRKFDFLVIGGGIAGISYALKVAEYGKVALICKTTLEESNTSL
                     AQGGMAAVLYPPDNFEKHIQDTLIAGDYINNIEAVKKVVYEAPKQIEELIRWGVKFDK
                     AEDGKFDLHREGGHSEFRILHHKDNTGAEVQKRLNKKVLHHPNIEIFEYHFALEILTQ
                     HHLGQIVTHHTPNIECYGAYILDQKNNQIITFLSKVTMLATGGIGGAYLTSTNPPTAT
                     GDGIAMVHRARGVIKDMEFVQFHPTALYHPGDKPSFLISEAIRGYGAILRTKDGQKFM
                     HKYDERKELAPRDIVARAIDNEMKIRGDEYVYLDVTHKDPKETKKHFPTIYKRCRELG
                     INITKDYIPVAPTQHYLCGGIAVDLNAESTIHRLYAAGECSCTGLHGANRLASNSLIE
                     AIVYADAAAKHSVVRLNSLGFQENIPDWDDEGTTWPEEMILITQSAREVEQIMNNYVG
                     IVRSNLRLKRAMSRLEILFRETEDLYERSVVSRQICELRNIISVSYLIIKQATQRKES
                     RGLHYNIDYPGHAEDLKNKE"
     gene            10805..11194
                     /locus_tag="PLD44_03845"
     CDS             10805..11194
                     /locus_tag="PLD44_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOF98502.1"
                     /translation="MKRTMLIVFWLALATMLLAGSYSPDDPRSSAQQVVVKSDVAVGQ
                     NWLRQDNSSGASDNQNQNIDNVSFSATSEGIYLSLGSGTYNVKLFALTGQLLWSGDLV
                     QGKFFIPVRKGIYFLRINGKSFKVVCK"
     gene            11266..11571
                     /locus_tag="PLD44_03850"
     CDS             11266..11571
                     /locus_tag="PLD44_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOF98503.1"
                     /translation="MVKHIVMFQLSDFSEGKTKKENATLIKSQLENLQHLIPELKKIE
                     VGIQMEETAAENYDLVLYTEFENVEDLQIYINHPEHKKVSAYIGKIKTGRVAVDYKV"
     gene            complement(11663..14122)
                     /gene="pheT"
                     /locus_tag="PLD44_03855"
     CDS             complement(11663..14122)
                     /gene="pheT"
                     /locus_tag="PLD44_03855"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOF98504.1"
                     /translation="MNISYNWLKKYINIDLPVEKVSEILTSIGLEVGSTEEVQTVKGG
                     LEGLVIGEVLTCVPHENSDHLHVTTVNIGSGEPLQIVCGAPNVAAGQKVVVATIGTKL
                     YAGDESFTIKRSKIRGVESFGMICAEDEIGIGTSHEGIIVLPDDAKPGMLAKDYYGIK
                     SDYVMEIDLTPNRVDAASHFGVARDLAAYFSLRDKSIKLTLPSVDNFQVQQHGLTIPV
                     TVENPEACPRYSSVTITGVKVGESPKWLKNYLTTIGLRPINNVVDATNFVLYELGQPL
                     HSFDADKIKGGKVIVRHMPEGTTFVTLDGVERKLSAEDLMICNAEEPMCIGGVFGGLD
                     SGVTESTQNVFLESAYFNPVYIRKTARRHGLSTDASFRFERGCDPNNTIYALKRCALL
                     IQEIAGGEIAGEIVDVYPKKILPFEVDITIAKINALIGKNFEKDTIQTILSALEIEIK
                     SSSEDGFVLKIPTYRVDVQRDVDVIEDILRIYGYNNVEIGEKLVSTLSYRPKPDSFRI
                     QNIISEQLTAQGFNEVMNNSLTKRNYYALSATLPLEKCVKIMNPLSSDLSVMRQTLLF
                     GGLENIVYNNNRKNLDIKFYEFGNCYFYLPENKNSSDPLSAYLEEFHLGLWLSGNKHP
                     QSWTSSEEKSSIFELKAYVENIFRRLGLKLQHFPFEEISNDLFSQALSFSNAAGKQLA
                     LYGIVNAALLKSFDIENEVFYADIDWNLLIEETKGKQVSFAEISKFPEVKRDLALLLD
                     KSVTFAEIEKIAYQTERKLLKKVQLFDVYEGKNIEAGKKSYAVSFVLQDETKTLTDSQ
                     IDAIMQKLLKNFTEKLGAKLR"
     gene            14460..14532
                     /locus_tag="PLD44_03860"
     tRNA            14460..14532
                     /locus_tag="PLD44_03860"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14493..14495,aa:Gly,seq:gcc)
     gene            14568..14651
                     /locus_tag="PLD44_03865"
     tRNA            14568..14651
                     /locus_tag="PLD44_03865"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14602..14604,aa:Leu,seq:cag)
     gene            14676..14759
                     /locus_tag="PLD44_03870"
     tRNA            14676..14759
                     /locus_tag="PLD44_03870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14710..14712,aa:Leu,seq:gag)
     gene            complement(14777..15694)
                     /locus_tag="PLD44_03875"
     CDS             complement(14777..15694)
                     /locus_tag="PLD44_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOF98505.1"
                     /translation="MKNEDRTSQAMHATWMGFWVNFTLTVFKLLAGFLGKSTAMIADG
                     VHSLSDFITDLIVIIFVRIAGKEKDQDHEYGHGKFETFATFLVSLALAAVGVYIFING
                     LNKVIQVTQGEIIEPPTIIALIAALVSIIVKEGLYRYTARIGKKINNQAIVANAWHHR
                     SDALSSIGTGLGIAGAIFFGEQARILDPIAGIIVSFFIIGVALKILLPSVNELLERSL
                     PEETKKEIFEIIESNPEILSYHNLKTRKVGNTFAIDIHIKLDKTTTFLRSHDIATEIE
                     NKLRNRFGKQTIINIHTEPFNEEKDQKRR"
     gene            16247..17461
                     /locus_tag="PLD44_03880"
     CDS             16247..17461
                     /locus_tag="PLD44_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOF98506.1"
                     /translation="MFRNQKITDDIYYVGVNDRQKAKFENLIPLYDGVSYNAYLIVDE
                     KTALIDTVDIHFGEILIQKILSQLGDKGLDYLIINHMEPDHSGSLRLLRKYFPNMTIV
                     GNERTLSMVKGFYEIDGSLLKVSDEESLSLGRHQLQFFLTPMVHWPETMMTFDHADKV
                     LFSGDAFGSFGALNGSILDENLDIDKYWDEMYRYYANVIGKYSSPVQKAIKKLEHLPY
                     EIICSTHGPVWKINLKKAVEIYDRLSSFQAEEGVVIVYGSMYGNTELMAETVAQGLAE
                     AGVKPVIIHNISTVDLSIILRDIYRYRGCIFGSPTYNNGLFPEMEDLIRKIESRGLKN
                     RIFGCFGSYTWSGAVVRNLTEFANRLQWPMSDVMVEEKQGLKKDNYESCLQLGHQIAE
                     MLQNPVQFNKGC"
     gene            17540..17767
                     /locus_tag="PLD44_03885"
     CDS             17540..17767
                     /locus_tag="PLD44_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOF98507.1"
                     /translation="MENRHSSGLGKINLVLIASGFAIIVIGFFLMTGSSTQVEFNPDI
                     FSFRRITVGPMVSFFGFLFIIFAILFKPKSK"
     gene            17770..18654
                     /locus_tag="PLD44_03890"
     CDS             17770..18654
                     /locus_tag="PLD44_03890"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOF98508.1"
                     /translation="MTFWQTIIIAIVEGLTEFLPVSSTGHMIITQSFLGIESTEFVKA
                     FTVIIQFGAILSVIVLYWNRFFRLQPLDEFNVPTYSEGKKFVKQRVIIRKFFYRYDFY
                     WKLLIGFLPAAVIGFLAGDFIDVLLGDVFVVALMLFIGGFVMLFVDKWFSASSQDQSI
                     GWQRALKIGFFQCIAMIPGVSRSMATIVGGMAVKLTRKNAAEFSFFLAVPTMAAASGY
                     KLFQLLSEQGGKDLLVQNWLLLLLGNVIAFIVALVAIRFFIDFISRYGFKAFGYYRIA
                     VGGVILLLLGMGYNLNMM"
     gene            18655..19353
                     /gene="truB"
                     /locus_tag="PLD44_03895"
     CDS             18655..19353
                     /gene="truB"
                     /locus_tag="PLD44_03895"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOF98509.1"
                     /translation="MDVFKGEVFYVDKPLHLTSFNVVNRLRWKIKKVAGIKKIKVGHA
                     GTLDPEATGVMIICTGKATKQIESFQSQTKEYIAELKLGATTPSFDMELPVNAVYPTE
                     HITRELIDQVVSTFIGEIEQIPPIFSAVKIDGKRAYEYARNGQEIELKPKKLVIDEIE
                     ILNFVPPTLTLRIVCSKGTYIRALARDIGFALNSGSFLTSLRRIRIGNVRIDDCWDIE
                     NLLNHLDKELKSTE"
     gene            19415..20464
                     /gene="queA"
                     /locus_tag="PLD44_03900"
     CDS             19415..20464
                     /gene="queA"
                     /locus_tag="PLD44_03900"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOF98510.1"
                     /translation="MKLSQFKFKLPDELIAQYPPKHRDEARLMVVHKKTGEIEHRIFK
                     DVLDYFNENDLFIFNDTMVFPARLYGNKEKTGAQIEVFLLRELNAEMRLWDVLVEPAR
                     KIRIGNKLYFGEDDSIVAEVIDNTTSRGRTLRFLFDGTHEEFKKALYALGETPLPHYI
                     KRQVEAADAENFQTIFAKNEGAVAVPAAGLHFSRELLKRMEIKGIDMAFVTSHMSLGI
                     FRDIDVEDLTKHKMDSEQIIVPEETAKKVNKAQEEGRNICAVGVTVMRALETVAGTEG
                     KIKSFDGWTNKFIFPPYDFSVANAMITNFQFPYSTLLMLTAAFGGYDTIMHAYEVAIE
                     EKYRFGVYGDAMLIV"
     gene            20647..21846
                     /locus_tag="PLD44_03905"
     CDS             20647..21846
                     /locus_tag="PLD44_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOF98511.1"
                     /translation="MPHTSQRGQLMPESPIRKLVPLADKAKARGVKVYHLNIGQPDLK
                     TPQVALDTLKNIDLKILEYSPSDGFKSLRSKLAGYYDKFNIEATEEDIIITTGGSEAV
                     NFAFMSCLDPGDEIIVPEPAYANYTAFAIAAGVVVRPVVASIEDGFALPPIEKFEELI
                     TPKTKGILICNPNNPTGYLYTRAEMNKIRDLVKKYDLYLFSDEVYREFCYTGGPYISA
                     FHLDGIDENVILVDSFSKRYSECGIRVGALVTKNKEVRKNVMKFCQARLSPPLIGQII
                     AEASIDTPAEYMLEMYNEYLERRKFLIDGLNRISGVYSPIPMGAFYTVARLPVDDADK
                     FCAWLLSDFDYEGSTVMLAPGSGFYTIEGMGKNEVRIAYVLEKEDLKKALVVLEKALQ
                     AYPGKTI"
     gene            21929..23170
                     /locus_tag="PLD44_03910"
     CDS             21929..23170
                     /locus_tag="PLD44_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF98512.1"
                     /translation="MNVEYFIAKRIHFQQGKKNVSRPAVRIATIGIALGLAVMLISVC
                     VVIGFKSQIENKIIGFGGHIQITNFDNNNTYEMNPIKMDAALIQKIRQIPGVVHVQRF
                     ATKPGIIKTDNEFQGIILKGIDKDFDWNFFRSNLLEGNILNVGSDSLKNEILLSKKLS
                     DLLGLKLGDELFMYFIQDQVRARKFKIVGIYSTNFVDYDQLFVLTDIRHVQRLNNWDS
                     ASFGGLEVLIDNFDKIDERGEAVYGATANIFSKDGNAYYTQTIKQLNPQIFSWLDLLD
                     MNVWVILLLMLSVAGFNMISGLLILILERTNMIGILKSLGATNWSVRKIFLYHSFFLI
                     AKGMFWGNLIGLSICALQYFTGIIPLDPEAYYVASVPVAFPWGYIVLLNIGTFVASIL
                     MMIAPSYLITKISPAKIIRYE"
     gene            complement(23349..>24432)
                     /gene="corA"
                     /locus_tag="PLD44_03915"
     CDS             complement(23349..>24432)
                     /gene="corA"
                     /locus_tag="PLD44_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOF98513.1"
                     /translation="TKKISHMAKYIKKRKKDIGVSPFELIFRGEKKMEKPRLYLMTYD
                     ENSVVETEIKSLRELNFNHSKDKFHWLNVEGLHEVKLMEKIAESFDIPFNIMSNIMDP
                     MTRPQVQDYEKGLFIVIKMLTYNEKLDKLDVENLSFILTKNTLILFQENQGDAFDPIR
                     ERLRKHHHRIRTAGIDYLACALLDVVIDNYLYLIGQLGDKIEDVEELMTKELDNDIPS
                     LINRYKRELNYLRKYIMPAKEMILNLSKLESEFIRDDNHPYFKELQVNLNEVSELMDS
                     YREILYDQLSIYHNTLSSRLNDIIKILTIFSVLFIPLTFIVGVYGTNFDYFPEIHWQY
                     GYFAMWAVMLLIAGVMLWYFKKKKWL"
BASE COUNT         7419 a         4359 c         4795 g         7859 t
ORIGIN      
        1 aaaaaagaga aaaaataaga tttttttgaa gagaggcatt aaattttttg ttgttatatt
       61 agcccttaaa aaaaacctga tgaaagcatt gggcatacca ttgttattgc aataaagagg
      121 cttgaaaatt tcttcatcga tgtgtatgag aacttctctt cggaaaagat tgtgccagga
      181 atcttccctt tgataataat tcccagctgt tccctctcat aagttcttcg ggcgaaaaaa
      241 ataaatctaa ttgtacttca ctttctgttt tcctgaacat ttatctctct tttttatcgt
      301 attttaacat gctaaggtgc tgaaatatag caatatatgc aaattttcaa agaatttttt
      361 tgaatatttt tttaactttt ttatctcaat gtctttaaaa aaactaattc tctttgaata
      421 acttttcgga gttgattctt aatttgtggt gtaaaaattt gaattttcaa taagaaaatc
      481 tacagaatga ctaaaaatac gtaaatttgc agaaagtggt tatttttgaa tctcaatatt
      541 cttttcagca attacttatt ttaaacacaa aaaggcagcc tttttgctgc cctttgtggg
      601 tggaggacgg gactcgaacc cgcgacattc ggaaccacaa tccgacgctc taccagctga
      661 gctacgacca ccattttttg cgagtgcaaa gataacacag catttgattc tgtgcaacta
      721 ttttaaaaaa aaattgacgt cttttggttt attgccgatt ttttggataa atgtttaaat
      781 gaaatgaata agcagacaat cgaacaaaac aatgaggtta aggagatttt acaaaaactt
      841 tggctttccc gaaacggaga agtagcttcc caaatgcgac agtacgggat ggtgtaccgc
      901 aaaaatctgg gcgtcgcgat aacccgcata agggaaatag cttccgatta tgagccaaac
      961 gaagaaacag cacaacaact ttggacactt cacggcagag aaaccatgtt gatggcaatc
     1021 ctgcttcagc ctgtaaacaa ttttagttgg gaaatggctc aaagttgggc cgaagactgc
     1081 gacaacatag aaacgattga atttgcgtgc atgcatcttt tctcaaaact cccttttgtc
     1141 aacgaacttt gctgtgattg gtgcgcttcg gacaaagttc aattgcaaat aatgggattt
     1201 attttatcgg cacgcaatta tcaaaatttt tcggatgtgg atagaaatct tattattgaa
     1261 aaggcttttg aaaattcgac aaccgataat ttccatctgt ataaagccat cagtttggcg
     1321 ctttgcagat tttgcagaaa tggaaaatcc atcgcggatt ttattctcga aaaaatgagc
     1381 aacttccttc ctcaaccaac tgccggtcaa caatatatca tggaagaagt gaaatcggaa
     1441 atgaattttt tggaactttt ataacaatta tattctaaaa aatcttacct ttgttctatg
     1501 gatgaaaaca aagaaaaaga agaaaattcc tttgaagtgg tcaagaaaat ttttaacgat
     1561 tatttggctg cccatcagca tcgcaaaacg cccgaacgtt acgcaattct ggagcaaatt
     1621 tacacccacg aaggacattt caatgccgag ctgctttaca atgccatgca aaaggattat
     1681 cgtgtcagtt tggccactat ttacaatacg ctcgatttgc tgctcgagtg caaattaatt
     1741 gtaaaacatc aatttggcga tcaactggca caatatgaaa aaactttcgg caccaacatt
     1801 catcatcatc ttatttgtac gcaatgtggc aaaataaagg aattttccga caaaaatata
     1861 aaagcagcca tccagtcgaa gcattttcaa acttttcaaa tgtcgcatta ctcgctgtat
     1921 atttatggaa tttgcagtaa atgcctgaaa aaaaagaaaa aaacttcgta aaaagtgttg
     1981 tttcaatgaa aaaaattacc ttatttttca ccttttctgt aaatggatgc cgtaagcaaa
     2041 cggattctga agcaaggaaa ggttttattt tgcaaaatca taaaaattta aacgaatgaa
     2101 agcagatgtt ttattaggcc ttcagtgggg cgacgaagga aaaggaaaag ttgtggatgt
     2161 actgacgcca cattacgata ttgtggctcg atttcaaggc ggccccaatg ccggtcatac
     2221 tttggagttt aaaggaaaaa aatttgtatt gcgttcggtt ccgtcgggaa tttttcaggg
     2281 cgacaaaatc aacgtgattg gcaacgggat ggtgattgac cctgcccttt tcaaggaaga
     2341 agtggaagct ctcgaagcag caggcgaacc cttgcatcag cggctgaaaa tttccaaaaa
     2401 agctcatctc attcttccga cgcatcgatt gctcgatctg gcttccgaag ctgccaaagg
     2461 aacaggcaaa attggtacaa cgggcaaagg tattggccct gcttatacag acaaaataag
     2521 cagaaatgga cttcggatag gtgatattct gcatcgtttc gacgaaaaat atcgtgaagt
     2581 cgttgctcgc catacggaaa tgttgcgtca atacaatttt gaatacgatt tggctgcatt
     2641 ggaaaaaact ttttttgaag gaatagaatg tatcaaacgt tttgatttga ttgacagcga
     2701 atactttatc aaccactcgc tggaagaagg gaaaaaaatt ttggccgaag gtgctcaggg
     2761 aacgttgctc gacattgatt tcggttcgta tccttttgta acttcttcca acacgatttg
     2821 tgccggcgct tgcaccgggc ttggcctctc tccgcaaaaa atcggagaag tttacggtat
     2881 tttcaaagct tattgcacgc gcgtgggaag cggtcctttt ccaaccgagt tgaaagacga
     2941 aaccggagaa ctgattcgca aaaaaggttg tgagtttggt gctgtaaccg gccgtcctcg
     3001 tcgttgtggt tggatcgatt tggttgcatt gcgctattca atcatgctca atggcgttac
     3061 tcagttgatt atgatgaaaa gcgacgtgct ggatgatttt gataccataa aggcttgtgt
     3121 ggcatatcgt atcgacggaa aggaaacgga agaatttcca tttgaaatta ccgaaaatct
     3181 cgaacccgtt tatgttgaat tgcccggttg gaaaaccaaa atatcgaact tgcaaagtga
     3241 agacgaattt cccgaagaat tgaatgccta tttgaatttt ttggaagaag aattgaatgt
     3301 cccgataaag attgtttctg taggacccga ccgtgaacaa accgtcattc gctatcagga
     3361 atagttttcc aatttttatg cactacaaac tgccaatctg tcaattaaat ttttggcaaa
     3421 cattttgcat gaaataaaat gttaattata aaattgaaaa gactatgatt tatattattt
     3481 ttattgtttt tgcgctgatt agttttttgc ttcagaacag gttgcagtca aaattcaaac
     3541 gttactcgaa attacctgta ccaaatggta tgacgggaaa agatgtagcc gaaaaaatgc
     3601 tgcacgacaa cggcatttat gatgtgaagg ttatttccac ctccggtcat ctcaccgatc
     3661 attataatcc ggcaaacaaa accattaatt tgagcgaaca ggtgtacgcc tcgagtaatg
     3721 tggcggcggc ggccattgct gctcatgaaa cagggcacgc ggttcagcac gcttatgctt
     3781 atgctccact gactctgcgt tcaaaactgg ttccggtggt aagttttgct tccatgtgga
     3841 tgcaatgggt actgttggca ggtattattt ttcttcaatc ttttcctcaa ctgatgttga
     3901 cgggaattat tctttttgcg ctgaccacgc ttttcagttt cataacttta ccggtagaaa
     3961 tcaatgccag tcaacgtgcg ttggcctggc tcaacaatgc cggcattacc aattatgaaa
     4021 cccacgaacc ggccaaagat gctttgaaaa cggcagctta cacttatgtg gtagccgctt
     4081 tgggatcgtt ggcaacgctg gtttattata tcctgatttt catgggtgga agccgaagat
     4141 aaatataaaa attcatacat tttgataagg aaaaccgact tttttaagag aaggttttcc
     4201 ttatttcttt tttcaaatcg ggaaaaatca ctacatttgc atccgcattg gctccgtagt
     4261 tcagttgtat agaacgtcag attccggttc tgaaggtctt gggttagaat cccaacgggg
     4321 tcacacacag ccaaccttaa catgtttgtt cgggttggct tttttggtaa gaaaattttt
     4381 atttgaaaat aattgtatgt tcttcacttt cagccgattg gctcaaagtt gttacacttc
     4441 caattttttc gttcaaaatc actaattttg cagaaaaatt ttaatattga atcctgctca
     4501 atcaatgaga aaaaagaaac agctccccgt tttggaaaag gtaaccatta ccgatattgg
     4561 agccgaaggc aaagcgctga caaaaataaa cgatatggtc gtttttgttc ctttggtggt
     4621 gccgggcgat gtagtggact tgcaagttac gcaaaagaaa aaaaattaca tggaagcccg
     4681 agctgtaaaa tttcatcact attcggaaca acgttgtgag cctttttgtc aacattttgg
     4741 catttgcggc ggttgcaagt ggcaaatgtt accttatgca gaacagttga aatacaaaca
     4801 aaaacaggta gaagatcatt tgaagcacat tggaaaagtt caattgccgg aaatttcgcc
     4861 cattctccct tccgaaaaaa ccgtttttta cagaaataaa ctggaattta ccttttccaa
     4921 tcgccgttgg cggagttttg aagaaatatc ttctgcacag aatattgaag atgaaaatgc
     4981 actcggattt cacattccgg gacattttga caaagtgttg aacattgaaa aatgctggtt
     5041 gcaggacgat atttccaata aaatcagaaa cgaaattcgt cgttatgctt tggagaatga
     5101 attggaattc tttaatctgc gtcgtcagga aggatttttg cgaaccatga tggtaagaac
     5161 ttccaccact ggtgaagtga tgctgctagt cgtgtttttt cgggaagacg aaacgaaacg
     5221 aaaagctctt ttgcaacaca tagccgacac ttttcccgaa attacttccc ttttgtacgt
     5281 aatcaattcc aaagccaacg ataccattac cgatcaggaa ataatttcct tcactggaaa
     5341 tgatagtatt tacgaaaaaa tggaagattt aactttcaaa attggggcaa agtccttctt
     5401 tcaaaccaat tctgcacaag cttgcaggtt gtatgaaaaa gcccgcgact ttgcagcatt
     5461 gacgggaaat gaacttgttt acgatttata caccggtacg ggcaccatag ccaattttgt
     5521 ggcgcgaaag gcaaagaaag tgattggaat agaatatgta cctgaagcag tggaagatgc
     5581 cgtgaataat tcaaaactta acaacattac caatacattt ttttatgcgg gtgatctgaa
     5641 agaagtaatg acagccaatt ttattcgtga gcagggaaat cctgatgtca ttatcactga
     5701 tccgccaaga gccggaatgc atggggatgt agtggatgcc attctgacta ctaatgccgc
     5761 aagaattgtt tatatcagct gcaattcggc aacacaagct cgtgatttgc aacttttgga
     5821 cagcaaatat aaagttactg ccgttcagcc ggtagatatg tttcctcata cgcatcatgt
     5881 tgaaaatgta gtattgcttg aacatcggta aacagaatat tttaaatgta tttgttttgt
     5941 acgagccttt ggaatctttt gttatcatta taacaaattg ttaattagtt ataaagatac
     6001 aaaatttcaa ggtgttcacc aaaaaatgac aattatttta ttgattatca gataatcata
     6061 taactataat ataatttttc tcatgtgcta aaatatattt gaaaaaactt atagcttaaa
     6121 ttttaaatta tcatgattag tttttttatt tccattggac ttttaatagc aggttatttt
     6181 atttatggaa aaattactga acgtattttt ggcattgagc cgaacagacc tactcctgct
     6241 cttgccaatc ctgatggcgt agattatatt cctttgccat ggtggcgggt atttttgatt
     6301 caatttttaa acattgctgg tttaggacct atctttggtg caattatggg tattatgttt
     6361 ggcgaatcag cttttttatg gattgtattt ggaaccattt tcgctggtgg agttcatgat
     6421 tatctttctg gtatgatgtc cattcgtaac aatggtacaa gtttacctga attggtagga
     6481 aatgaactgg gaaatggtat caagcaattt atgagggttt tttcgctggc tttgatgata
     6541 ctggtaggtg cagtttttgt ttcaggtcct gcacaacttc tggcaaattt tactcctcat
     6601 tctattaatg taagtggatg gattgtaatt gtatttacct attatatttt agctactctg
     6661 ttgcccattg ataaaattat tgctaaaatt tatccatttt ttggttttgt cttattattt
     6721 atggctgttg gaattttttt agctcttata ctaaataaag ctcctatccc ggaatttaca
     6781 gaaggatttg ccaatcttca tcctaataag ttacctattt tccctattat gtttgtttcc
     6841 attgcttgtg gtgctatttc aggatttcat gcaacccaat ctccacttat ggctcgctgt
     6901 attcaaaatg aaaaattagg acgtcgtatt ttttatggca gcatggttgc cgaaggaata
     6961 gtagctttaa tttgggctgc agcagcttcc agtttttttg gttccattca aggattacaa
     7021 gaatttctgg gaactcttcc ggctactgct aataaacctg ctgtggtggt agatcgtatt
     7081 tgtagagatt ggctgggaaa tattggagga gttataacat tattaggtgt aatagctgct
     7141 ccactcactt caggcgatac ggcattgcgt tcagcgcgac tgattgtagc tgactttatt
     7201 catctggagc aaaaatcttg gaaaaaaagg ttgatgattt ccattcctat cttcattttg
     7261 acttttattg tcctacaaat taattttgat gctttgtgga gatattttgc ctggtgtaat
     7321 cagacattgg ctgtttttac actctggtct atcactgttt atctggcaaa aaggcacaag
     7381 ggttattgga tttctcttat cccagcagta tttatgactt gcgttagtgt aagttatatt
     7441 tgcattgcac ctgaaggatt tcaattgcct cagtatcttt cttatcttat cagtgcaatt
     7501 gcttgtgttg tggttatatt tttcttttat cgttttattt accttaataa ggaaaaatta
     7561 ttaactgaaa atcaattatt taataaaaaa tgattcaaaa aagttcaaaa ttgaatcttt
     7621 cccgttgaaa attgactaat tttacatttt tttttaacag ataaaaagta ctttctaaaa
     7681 agcagtatta ctgtaattac ttatttttgg caaagaatga aaaaagacat tctgatgaaa
     7741 aagcaaataa aatatttttt ttaaaaacct aaacggatat ggcgataact gttcgagaag
     7801 taactaccaa aaaacaattg aaacaatttg tctggtttgg gatcaatttg tacaaggata
     7861 atgaatatgc agctccaccg ctgtttatag acgactatac caacctgact aaaggaaaaa
     7921 acccttcgct tgatttttgc gaagctgctt attttcttgc ctacaacgaa aaaaatgaaa
     7981 ttgttggtcg cattgcagcc ataatcaatc cggttgccaa tgaaacatgg aatcagaaaa
     8041 atgcacgttt cgggtgggtg gattttatcg acgactatga agtttcgaaa gccctgtttg
     8101 atgcggcagt ggaatgggca agagccaaag gcatgacagc catgcaaggc ccactcggat
     8161 ttaccgattt tgaccacgaa ggtacgctta tcgaaggttt tgaccgactt ggaacattgg
     8221 ccggcatata taattatcct tattattcac agcacatcga aaaatatggt tttgtgaaag
     8281 atgccgattg ggttgaattt ctcattacca ttcccgaaaa atttcccgat aaatatttcc
     8341 gtatcgctga gctggtgaaa caaaaatatc acttaacacc tcgacatttt aaaagccgta
     8401 aaaaactggt aaacggctat ggaaacaaaa ttttcgattt actcaatact tgctatcagc
     8461 atttatacgg tttcaccaaa ttgaacgaag cccaaaagca gttttacata aaactctatt
     8521 tcagcttttt cagactggat acggtttcgg tggtaaccga cgaaaatgat gatgtggtag
     8581 cagtgggtat tgccatgcca agtctgacca aagctttgca gaaagcaaaa gggaaaatat
     8641 tcccgctggg ttggttttat ttgctgaaat ctatttatta taaaaacgat atcgtggatt
     8701 tgtatctgat agcagtacat ccccgttatc agggaaaagg tgtcaattct atcctttttg
     8761 ccgaattgat ggtcaatttt gcaaaaaatg gatataaatt tgcagaaagt aatccggagt
     8821 tggaaaccaa tactcaggtt tcctctcagt ggagtgattt tgaacatatc aatcacaagc
     8881 gcagaagagc ttacattaaa caaatctgaa aatttacttt ttcttgaaaa aattatttaa
     8941 ttcgtttttt tgaccaacta tgaaattttt tgaatggttg gactccacct atcaacttct
     9001 ttcaactttt ttagctcgaa ttttgtttga atagtgtgaa gtcaataaac aattgactta
     9061 atacatattg attatgacac gaaaatttga ttttttggta ataggaggtg gaatagccgg
     9121 catcagttat gcactcaaag tagctgaata cggaaaagtt gccctcattt gtaaaaccac
     9181 acttgaagaa tcgaatactt ctttggctca gggaggtatg gcagccgttc tttatccacc
     9241 cgacaatttt gaaaaacata ttcaggatac tttaattgca ggtgattaca taaataatat
     9301 cgaagctgta aaaaaagttg tttacgaagc ccccaaacaa attgaagaat tgattcgctg
     9361 gggtgtgaaa ttcgataagg cggaagacgg aaaattcgat ttgcatcgcg aaggcggtca
     9421 ttcggaattt cgtattctcc atcataagga caatacagga gcggaagttc aaaaaagatt
     9481 gaacaaaaaa gttctacacc atcccaatat cgaaattttt gaatatcatt ttgctctcga
     9541 aattttaact cagcatcatt tgggtcagat tgttacgcac catacgccga atatcgaatg
     9601 ttatggcgct tacattcttg atcaaaaaaa caatcaaatt attacttttc tttcaaaggt
     9661 taccatgttg gcaaccggag gtattggagg tgcatatctg acttctacca atccgcccac
     9721 tgccactggt gacggcattg caatggtaca cagagccaga ggtgtaataa aagacatgga
     9781 atttgtgcag tttcatccca ctgcacttta ccatccgggt gataaaccct ctttcctgat
     9841 ttcggaagcc atacgtggct atggagcgat tttgcgtacc aaagacggac aaaaattcat
     9901 gcataagtat gacgaaagaa aagagctggc tccccgagac attgtagccc gcgctatcga
     9961 caacgaaatg aaaatacgcg gtgacgaata tgtttatttg gatgttacgc acaaagaccc
    10021 aaaagaaaca aaaaaacatt tccccactat ttataaaaga tgccgcgagt tgggtatcaa
    10081 cattaccaaa gattatattc ccgtagctcc aacacagcat tatttgtgtg gtggcattgc
    10141 tgtggatttg aacgccgaat caaccattca tcgtctgtat gcggcaggag aatgttcctg
    10201 taccggttta catggagcca atcgtttggc ttccaattct ttgatcgagg caattgttta
    10261 tgctgatgct gcagccaagc attctgttgt ccgacttaat tcacttggtt ttcaggaaaa
    10321 tattcccgat tgggatgatg aaggcacaac ttggcctgaa gaaatgattt tgattactca
    10381 aagtgcgcgg gaagtagagc aaatcatgaa taactatgta ggaattgttc gttccaattt
    10441 gcgtttgaag cgggctatga gccgattgga aattcttttc agggaaacgg aggatttata
    10501 tgagcgatcg gtggtttcgc gccaaatttg cgaacttcga aacattatca gcgtttcgta
    10561 cttgattatc aagcaggcta cacagcgaaa agaaagtcgc ggattgcatt acaacatcga
    10621 ttatccggga catgcagaag atttaaaaaa taaggaataa cgacctaaat atttttcgat
    10681 aacgaatcac aaaataaact gaaagtttat gaaccggcga taaatttttt cccttttttc
    10741 tttggacgtt tttcactatt tttgcagtaa attatttctc aacctgttaa attttctatt
    10801 tgcgatgaaa cgtacgatgt tgattgtgtt ttggcttgct ttggccacga tgcttttagc
    10861 cggaagctat tctcccgacg atccgcgttc atcagcccaa caagtcgttg ttaaatctga
    10921 tgttgctgtg ggacaaaact ggttgagaca ggataactct tccggtgcat ctgacaatca
    10981 aaatcaaaat atcgacaatg taagttttag cgctacatcc gaaggaattt atttgtcatt
    11041 gggcagtgga acatataatg tgaaactttt tgcactcacc ggacagttgt tgtggagtgg
    11101 cgatttggtt caggggaaat tttttattcc ggtgcgaaaa ggaatttatt ttttgagaat
    11161 caacgggaaa agttttaaag tggtttgcaa ataaaagtaa tacttttgcc cccattaaaa
    11221 gaaaccggaa aaaactctat ccgataaatt ataaagaaat tttttatggt taaacacatc
    11281 gttatgtttc agctttccga tttttcggaa ggaaagacaa aaaaagaaaa tgcaacgctc
    11341 atcaagtcgc aattggaaaa tcttcaacac ttgatacctg aattgaaaaa aatcgaggtc
    11401 ggtattcaaa tggaagaaac ggctgcagaa aattatgatt tggtattgta caccgaattt
    11461 gaaaatgtag aggatctgca aatttatatc aaccatcccg agcacaagaa agtcagcgca
    11521 tatatcggta aaattaaaac cggcagagta gcagtagact acaaagtttg atcaaaaaaa
    11581 aacaggaaaa agaaaagatt tttctttatc acaagcaaag gtgaaaagaa aaatcttttt
    11641 ttatgtatcg gtttgttctg cgttaacgga gtttagcacc cagtttttcg gtgaaatttt
    11701 tcagaagttt ttgcataatg gcatcaatct gactgtcggt aagtgttttt gtttcatcct
    11761 gcaatacaaa gctcactgca taagattttt tacctgcttc gatatttttg ccttcgtaca
    11821 cgtcgaacaa ctgaactttt ttcaaaagtt ttctttcggt ttgataggct attttctcaa
    11881 tttcggcaaa tgttacgctt ttatcgagca acagggccaa atcacgtttt acttcaggaa
    11941 attttgatat ttcggcaaaa ctaacctgtt tccctttcgt ttcttctatc aacaaattcc
    12001 aatcaatatc ggcataaaaa acttcatttt caatatcaaa acttttcagc agtgcagcat
    12061 tcactattcc gtacaatgcc agttgtttac cggctgcatt gctaaagctc aaagcttgtg
    12121 aaaacaaatc gttggaaatc tcctcgaagg gaaaatgctg caatttcaat cccagcctac
    12181 gaaaaatatt ttccacgtat gctttcagtt caaaaatact tgatttttcc tccgaagaag
    12241 tccacgattg cggatgtttg ttgcccgaca accaaagtcc caaatgaaat tcttccaaat
    12301 atgccgacaa aggatcggat gaatttttgt tttcgggaag atagaaatag caattcccaa
    12361 attcataaaa ctttatgtct aaatttttcc gattgttgtt gtaaacaatg ttttccaaac
    12421 ctccaaaaag cagagtttgt ctcatcacgc tcaagtccga acttaacggg ttcattattt
    12481 ttacacattt ttctaaaggc aaagttgcag aaagtgcgta ataatttctt ttggtcagcg
    12541 aattgttcat tacttcatta aaaccctgcg ctgtcagttg ctcggaaata atgttctgaa
    12601 ttcggaagct gtccggttta ggtcgatagc tcaacgtgga aaccagtttt tctccgattt
    12661 ctacattgtt gtaaccataa atacgaagaa tatcttcaat gacatccaca tcgcgttgca
    12721 catcgaccct gtaggtcggg attttcaaaa caaaaccgtc ttcggaagaa gattttatct
    12781 caatttccaa agccgaaaga atggtctgaa tggtgtcctt ttcgaaattt tttcctatca
    12841 aagcgttgat tttcgcaatg gtgatgtcca cctcgaaagg aagtattttt ttggggtaaa
    12901 catcaacaat ttctcctgcg atttctcctc cggcaatttc ctgaatcagc aaagcacagc
    12961 gcttcaaagc gtaaatggtg ttgttggggt cgcagcctcg ctcaaagcgg aaagaagcat
    13021 cggtactcaa tccgtgccga cgagccgttt ttcggatata aacgggattg aaataggcac
    13081 tttccagaaa aacgttttgt gttgattctg taactcccga atcgagtcca ccaaaaacgc
    13141 ctccgataca catgggctct tctgcattgc aaatcatcaa atcttcggcg ctcaatttac
    13201 gctctactcc atccagcgtt acaaaagtcg ttccttcagg catgtgtcgc acaataactt
    13261 ttccgccttt tattttatcg gcatcaaaag aatgaagcgg ctgccccaat tcatacaaca
    13321 caaaattggt ggcatccacc acgttgttga tgggacgcaa gccaatggtt gtcaggtaat
    13381 ttttcagcca tttgggcgat tcgccaacct ttacgccggt aatggttaca gaagaatatc
    13441 gcggacaagc ttccggattt tctactgtta ccggaatggt aagcccatgt tgttgaacct
    13501 gaaaattatc taccgacggc agggtaagtt ttatcgattt gtctcttaac gaaaaatagg
    13561 cagccaaatc acgagcaacg ccgaaatgtg aagcagcatc cacgcggttg ggtgtcagat
    13621 ctatttccat cacataatcg ctttttatgc cgtaataatc ttttgccaac attccgggtt
    13681 ttgcatcgtc cggcaaaact atgatgcctt cgtgactcgt tccaattcct atttcatctt
    13741 cggcacaaat cattccgaaa gattccacac cacgaatttt ggagcgtttt atggtaaaac
    13801 tttcgtcgcc ggcatacagt tttgtaccaa tggtagccac aaccactttt tgtccggcag
    13861 ccacattcgg agcgccgcaa acaatctgca aaggttcacc ggagccaata ttgacagttg
    13921 ttacgtgcaa atgatcagaa ttttcatgag gaacgcacgt cagcacttct ccaatgacca
    13981 atccttccaa cccacctttc acagtttgaa cttcttcggt gctgccaact tccaatccga
    14041 tagaagtcag aatttccgaa accttttcta ccggcaaatc gatgttgatg tattttttga
    14101 gccaattata cgagatattc atatttgttt gtttttatag tcaattattt ggaaagggtc
    14161 ttgatttttc cgctgcaaaa gtacatgaaa atcgaggtat ttccaacttc caactataaa
    14221 atcttgaaca aaaccttaat agcttgccat aggataatag ttgaacagtt tctattgata
    14281 ggttgcattt aatcaattac agaaggatac agctacggaa tatagaaaga aaaaagtttt
    14341 tgaagaatta gttacttgta ttttttaaaa gaaaaatatc aagatttttc ctttgaaatt
    14401 ttttgcaaat tagcaaaaag aaagtatctt tgctgcgctt ttccaaaaga tcaaattgtg
    14461 cgaaaatagc tcagttggta gagcacgacc ttgccaaggt cggggtcgcg ggttcgagtc
    14521 ccgtttttcg ctcaaaactt atccctgcaa ttttgaaaac aggaatagcc caaatggcgg
    14581 aattggtaga cgcgctagtt tcagggacta gtggccgtta ggctgtgcag gttcgagtcc
    14641 tgttttgggc acaccaagtt ttcattttgt tttttgcgca gatggcggaa ttggtagacg
    14701 cgctactttg agggggtagt gccggttacg gcgtgtgagt tcgagtctca ttctgcgcac
    14761 cctgagtaaa tcttgtttat cttctctttt ggtctttttc ttcgttaaat ggttcggtat
    14821 gaatattaat gatggtttgt ttaccaaatc gatttcttag cttattttca atttcagttg
    14881 caatatcgtg agaacgtaaa aaggttgtag ttttgtcgag tttaatgtgt atatcgatgg
    14941 caaaagtgtt tcctactttt ctggttttga ggttatggta agacagtatt tcagggttcg
    15001 attcgataat ttcaaatatt tcctttttcg tttcttctgg taatgacctt tctaaaagtt
    15061 cattgacgct gggtaataaa attttcaggg ctactcctat tataaaaaag ctgacaatga
    15121 tacccgctat cggatctaaa attcgagcct gctcaccaaa aaaaattgct ccggcaatac
    15181 ctaatccggt acctattgat gataaggcat cggagcgatg gtgccatgca tttgctacaa
    15241 tggcttgatt gttaattttt ttcccaattc ttgctgtata tcggtataat ccttccttaa
    15301 caataatact caccagagct gctatcaatg ctataatggt tggtggttca attatttctc
    15361 cctgtgttac ctgtataact ttgtttagac cgtttatgaa aatgtaaact cctacagcag
    15421 cgagtgcaag gctgaccaga aaagttgcaa atgtttcaaa ttttccgtgt ccgtattcat
    15481 ggtcttgatc tttttctttt ccagcaattc ttacaaatat aataacaatc aaatctgtta
    15541 taaaatcgga tagtgagtgt actccatctg caatcatggc agtactttta cccaaaaaac
    15601 ctgccaaaag tttaaatact gttaaggtaa aattaaccca aaaacccatc cacgtagcat
    15661 gcatggcttg tgatgttctg tcttcatttt tcatatttca cttcaatgat agaaaaatat
    15721 tcatgaatgc aaaaataata aataataaat ggttttttca cctatttttg aagtaacaag
    15781 ctaatttata agaagtttaa ataatatttt aaactattct aaattacttt attccagtca
    15841 aaattcactt aaaagcagta atcaaggaat caagtaagaa aattcttttt caacagaata
    15901 taatttattt ttaaaggtct gatggaaccc acatgtctga atattatatt cattcttgtt
    15961 tgtgtatgca acacatgtgg gtttcataaa caactctgta aaacctcata gctatttggt
    16021 tttctacaaa gttaaaactt tttcttttag gaatttttgt tttgatttac caaaaatgag
    16081 tttttcaaac attttatcta attttgcatt tgccggttga attttcctcg tcataaggct
    16141 gactctatat ttttcctatt tggagaattt attttatctt tgtagttcga ttttttattt
    16201 ttgaaatcac tatcattaca aactgagaag ttaaaattga ctaaaaatgt tccgtaacca
    16261 aaagataaca gacgatatat attatgtagg cgttaacgat agacagaaag cgaaattcga
    16321 gaatttaatt cccttatacg atggtgtttc ttacaatgca tatttgattg tagatgaaaa
    16381 aaccgctttg attgatacag tagatattca ttttggcgaa atattgatac aaaaaatact
    16441 ctcacaattg ggagataaag gattagacta tctgattatc aatcacatgg aaccagatca
    16501 ttcgggttcc ttgcggttgt tgagaaaata ttttccaaat atgactattg taggcaatga
    16561 aagaacattg agcatggtga aaggatttta tgaaattgat ggatcgcttt tgaaagtttc
    16621 cgatgaagaa agcctttctt tagggcgcca tcaattacag ttttttctga ctccgatggt
    16681 tcattggccg gaaacaatga tgacttttga ccatgccgat aaggtgcttt tttcaggcga
    16741 tgcttttgga agctttggtg cacttaatgg aagtatattg gatgaaaacc ttgatataga
    16801 caaatattgg gatgaaatgt atcggtatta tgctaatgtt attggtaaat atagttcgcc
    16861 ggtacaaaaa gccataaaaa aattggagca tttaccttat gaaataattt gcagcacaca
    16921 cggccccgtg tggaaaatca atctaaaaaa agctgtagaa atatatgata gactaagcag
    16981 tttccaagca gaagaaggag tagtgatagt ttatggatca atgtatggaa atacagaact
    17041 aatggctgaa acggtagcgc aaggtttggc agaagcaggt gttaaacccg tgattattca
    17101 caatatttca acagtcgatc tttccatcat tttgcgagac atttaccgat atagaggttg
    17161 tatttttggg agtccaactt ataacaacgg actatttcct gaaatggaag atttaattag
    17221 aaaaattgaa agtcgtggat taaaaaacag gatattcggt tgttttggtt cctatacgtg
    17281 gtcgggcgca gtggtgagaa acttgactga atttgcaaat cgcttgcaat ggcctatgtc
    17341 tgatgtgatg gtggaagaaa aacagggtct aaaaaaagat aattatgaat cctgcctaca
    17401 attaggtcat caaatagcag aaatgttaca aaatcctgtt caattcaata agggctgtta
    17461 accgcgtaac tttttgaaaa aggtaatatg ttttttcctg tgtatatttt aattttaatt
    17521 tgttaaattt tttaacgtaa tggaaaatag acattcatcc ggacttggca aaataaattt
    17581 ggtgcttata gcatccggat ttgcaattat tgttataggt ttttttctaa tgacaggttc
    17641 ttctacccaa gtggaattta atccggatat tttcagtttt cgtcgcataa ctgtaggccc
    17701 aatggtttca ttttttggtt ttttgtttat catatttgcc attcttttta aaccaaaaag
    17761 taaataatga tgactttttg gcagacaatt ataatcgcca ttgttgaagg actaacggaa
    17821 tttcttcctg tttcttcaac tggacacatg ataattacac aaagttttct gggtatagaa
    17881 agtacggaat ttgtaaaagc tttcactgtt attattcaat ttggagctat tctttctgtg
    17941 attgttcttt attggaacag attttttcgg ttgcaaccac tggatgaatt taatgtgccg
    18001 acctattctg aagggaaaaa atttgtgaaa caacgtgtta ttatccgaaa atttttttat
    18061 cgatacgatt tttactggaa actgttaatt ggttttttac ctgctgctgt cataggtttt
    18121 ttagcaggcg attttattga tgttttgctc ggagatgttt ttgttgttgc tttgatgctc
    18181 tttataggag gctttgtgat gctttttgta gataaatggt tttcagcctc ttctcaagac
    18241 cagtcaattg gttggcaaag agctttaaaa atcggttttt ttcaatgtat agccatgatt
    18301 cctggagttt cccgttcaat ggccaccata gtaggtggaa tggcggtaaa attaacgcga
    18361 aagaatgcgg cagaattttc gtttttcctt gctgttccta ctatggctgc agcttcaggg
    18421 tacaaacttt ttcagcttct cagcgaacag ggtggaaagg atttgctggt gcagaactgg
    18481 ttgttgctgt tattgggtaa tgtaatagct tttattgttg cattggttgc cattcgtttt
    18541 tttatcgatt ttataagtcg ttatggattc aaggcattcg gttattatcg catagcagta
    18601 ggaggagtta ttttattgtt gttgggaatg ggttataatt taaatatgat gtaaatggat
    18661 gttttcaaag gcgaagtttt ttatgtggat aaacctttgc acttgacatc attcaatgtt
    18721 gttaataggt tacgctggaa aattaaaaaa gttgccggaa taaaaaaaat taaagttggc
    18781 catgccggca cgcttgatcc ggaagcaacg ggcgtgatga ttatttgtac tggtaaggca
    18841 accaaacaaa ttgaaagttt tcagtcccaa accaaagaat atattgccga attaaaatta
    18901 ggtgcaacta caccatcctt cgatatggaa cttccagtaa atgcggttta tccaaccgaa
    18961 catatcacgc gagaattgat agatcaagtt gtgtcaacct ttattggtga aatagaacaa
    19021 atccctccga tattttcagc ggtgaaaata gatggaaaaa gagcttatga atatgcaaga
    19081 aatggacagg aaattgaact aaaaccaaaa aaattggtta tagatgagat tgaaatactc
    19141 aattttgttc ctcctacatt aacgcttcga attgtttgca gtaaaggaac ttatattagg
    19201 gctttagctc gcgatattgg ttttgctctt aactcgggat cctttcttac ttccttgaga
    19261 cgcatacgca ttggaaatgt tcgaatcgat gattgttggg acatcgaaaa tttgttgaac
    19321 catttggata aagaactaaa atcgacagaa taaaaattct tacattataa ggcttttgga
    19381 acaactttaa aaattaaatc tttagctgat aaatatgaaa ttatctcaat ttaaatttaa
    19441 attacccgat gaattgattg cccagtatcc tcctaaacat cgagatgagg cacgattaat
    19501 ggtagttcac aaaaaaaccg gagaaattga acatcgtatt tttaaagatg tgttggacta
    19561 tttcaacgaa aatgatctct ttattttcaa cgatacgatg gtttttcccg cccgtcttta
    19621 tggaaataaa gaaaaaacag gagctcaaat tgaggttttt ttgcttcgtg agttaaatgc
    19681 tgaaatgcgt ttatgggatg ttttggtaga acccgcccgt aaaattcgaa taggaaataa
    19741 actttatttt ggggaagatg attccattgt ggcggaagtt atcgataata ctacttcacg
    19801 cggtcgaacc ctacgttttt tgtttgatgg cacacacgaa gaatttaaaa aagcgcttta
    19861 tgccttagga gaaacacctc ttcctcatta tatcaagcga caagttgaag ctgccgatgc
    19921 agaaaatttc caaacaattt ttgccaaaaa tgaaggagca gttgccgttc ctgcggcagg
    19981 tttgcatttt agtagagaat tgttaaaaag gatggaaatt aagggaatag atatggcttt
    20041 tgttacttct cacatgagct tgggtatatt tcgagacatt gacgttgaag atttgaccaa
    20101 gcataaaatg gattccgagc aaattattgt tcctgaagaa acggccaaaa aagtcaacaa
    20161 agctcaagag gaaggaagaa atatctgtgc agtaggggtt acagtaatgc gagcactgga
    20221 aactgtagcc ggaacagaag gaaaaattaa atcctttgat ggctggacaa ataagtttat
    20281 atttcctccc tatgattttt cagttgctaa tgcaatgatt accaactttc agtttccata
    20341 ttccactttg ctcatgctaa cagctgcttt tggcggatat gataccatta tgcatgccta
    20401 tgaggttgct attgaagaaa aatatcgatt tggagtatac ggagatgcta tgttaattgt
    20461 ttaattattg acttcctact caatgtcata tttcattgac aggaaaattt tcaatacgat
    20521 aaaaaaacaa catttcattt ttatcgggta aattcattaa ttttggaaat cggttgaaat
    20581 ggtaaatttc atttattcag taccgaataa ttttatattt gttaatttgc aaatttaaaa
    20641 caacacatgc ctcacacttc acaacgtggg caactcatgc ccgaatctcc catccgcaag
    20701 cttgttcctt tggccgataa agccaaagca cgcggagtaa aagtttatca tttgaacatt
    20761 ggccagcccg atttgaaaac gccacaagtt gctttagaca ctttaaaaaa tatagatctt
    20821 aaaattttgg aatacagccc cagcgatggt tttaaaagtt tacgcagcaa attggccggt
    20881 tattacgaca agttcaacat tgaagcgacg gaagaagata ttattatcac taccggaggt
    20941 tcggaggcgg taaattttgc ctttatgagt tgcctcgatc cgggcgacga aattattgta
    21001 ccggagcctg cttatgccaa ttacacggct tttgccattg ctgccggagt ggtggtacgt
    21061 ccggttgtgg cctccattga agatggcttt gctctgccac ctattgagaa atttgaagaa
    21121 ctgataactc caaaaaccaa aggaatttta atttgcaatc ccaataatcc taccggctat
    21181 ctttacacgc gtgccgaaat gaataaaatt cgcgatctgg tgaagaaata cgatttgtat
    21241 cttttttccg acgaagttta tcgtgaattc tgttatacgg gtggtcctta tatttcggct
    21301 tttcatctcg atggcattga cgaaaatgtg atattggtcg attctttttc caaacgttac
    21361 agtgaatgtg gtatccgtgt tggtgcactg gtaacgaaaa acaaggaagt gagaaagaat
    21421 gtgatgaaat tttgtcaggc tcgtctcagc cctccgctta taggtcaaat tattgccgaa
    21481 gcttctatcg acacgcctgc tgaatacatg ctcgaaatgt acaatgaata tctcgaacgg
    21541 agaaagtttt tgatcgacgg attgaatcgt atttcgggag tttattctcc aattccgatg
    21601 ggtgcttttt atacagtcgc tcgcttaccg gttgatgatg ccgataaatt ttgtgcatgg
    21661 ctgctttccg atttcgacta cgaaggttct accgtgatgc tggctccagg ctccggtttt
    21721 tataccatcg aagggatggg gaaaaatgaa gttcgtatcg cttacgtgct ggaaaaagaa
    21781 gatttgaaaa aagctcttgt cgttctcgaa aaagctttac aagcctatcc cggaaaaaca
    21841 atctgatgtt ggcagatgaa aaagatttga acatttctct gaaaattcaa aatatttttg
    21901 ttcatcttaa tcgataattc ttattgattt gaatgttgaa tatttcatag caaagcgaat
    21961 tcattttcag caggggaaga aaaatgtttc ccgccctgcg gtgcgtattg caaccatcgg
    22021 cattgcgttg ggattggctg tgatgttgat ttcggtttgt gtggtgattg gtttcaaaag
    22081 ccaaatcgag aacaaaatca tcggttttgg cggacacatt caaatcacca atttcgacaa
    22141 caacaatacc tacgaaatga atcctataaa aatggatgct gctctgatac aaaagattcg
    22201 gcaaattccg ggtgtggttc atgtgcagcg ttttgccacc aaaccgggta ttatcaaaac
    22261 cgataatgaa tttcagggta ttattctaaa aggtattgac aaggattttg actggaattt
    22321 tttccgttcc aatttactgg aaggaaatat tttaaatgtt ggcagcgatt cgctgaaaaa
    22381 cgaaattttg ctttctaaaa aattgtccga tttactggga ttgaaattgg gcgacgagct
    22441 ttttatgtat ttcattcagg atcaagttag agctcgaaaa tttaaaatcg tcggcatata
    22501 ttccaccaat tttgttgatt acgatcagct ctttgtgctt accgacatcc gacatgttca
    22561 gcggttgaac aattgggaca gtgcatcgtt cggtggactt gaggtgttga tagacaattt
    22621 tgacaaaata gatgagcggg gagaagctgt ttatggagcc acagccaata ttttcagcaa
    22681 agatggcaat gcttactaca cccaaaccat taagcagttg aatcctcaga ttttcagttg
    22741 gctcgattta ttggatatga acgtttgggt aattttgcta ttgatgcttt ccgttgcggg
    22801 ttttaacatg atttccggtt tgctgatttt aattcttgaa cgtaccaata tgattggcat
    22861 tcttaaatcg ttgggtgcaa ccaactggtc ggtaagaaag atttttcttt atcattcttt
    22921 tttcctgatt gcaaaaggaa tgttttgggg taacctcatt ggcctttcca tttgtgctct
    22981 tcagtatttt acgggtatta ttccgctcga tcccgaagct tattatgtgg cttccgtacc
    23041 ggttgctttt ccgtggggtt atattgtatt gctgaatatc ggaacttttg tggcatcaat
    23101 tttaatgatg attgcgccat cttatcttat caccaaaatt tctccagcaa aaattattcg
    23161 ttacgaatag gtgcattttc tattaacaac cgaaaaatta ttgcttttca aattaagtgg
    23221 taataaccac atacatttta atggattgcc aaattaattt ctaaattaat acgcctaata
    23281 gtagtcgaac aaaatcattt ttaatggttt ttccttctca aaaaaattct agttttttag
    23341 gggctttttt acagccattt tttctttttg aaataccaca acattactcc ggcaatgagt
    23401 agcatgacgg cccacatggc aaagtagcca tattgccagt ggatttcagg aaaataatca
    23461 aaatttgtcc cgtacactcc aacaatgaaa gtaagtggaa tgaacagtac cgaaaaaatg
    23521 gtcagtattt tgattatatc attcaaacgg gagcttaggg tattgtgata aatgctcaac
    23581 tggtcgtata gaatttcgcg gtagctatcc attaattcgc ttacttcatt aagatttacc
    23641 tgtaactcct tgaaataggg atggttgtca tcccgaataa attccgattc gagtttggac
    23701 aggttcagaa tcatttcttt ggccggcata atatattttc tcaaataatt caattctctt
    23761 ttataccgat ttatcagcga aggaatatcg ttatccaatt ctttggtcat gagttcttcc
    23821 acatcttcaa ttttatctcc caattgtccg ataaggtaca aataattatc aattaccaca
    23881 tcgagcaatg cacaagccaa ataatcgatg ccggcagtac gaattcgatg atgatgtttt
    23941 cgtaagcgtt cgcgaatggg atcaaaggca tcgccctgat tttcctgaaa tagtatcaag
    24001 gtgtttttgg tgagtataaa actcaaattt tccacgtcca atttgtcaag cttttcatta
    24061 taagtaagca ttttgattac gataaaaagc cctttttcgt agtcctgaac ttgagggcgc
    24121 gtcatgggat ccatgatgtt cgacatgata ttgaagggaa tatcgaaaga ttcggcaatt
    24181 ttttccatca gttttacttc atggagtcct tcaacgttta accagtgaaa tttatctttg
    24241 ctatgattga aattcaattc gcgtaaagat ttaatttctg tttccaccac gctgttttca
    24301 tcataggtca tcaaatacag acgtggtttt tccatttttt tttcgccacg aaaaatcaat
    24361 tcaaaaggag agacgccaat atcttttttc ctttttttga tatattttgc catatgactt
    24421 atttttttag tt
//
LOCUS       DAOSIB010000026        24372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_2294_length_24372_cov_12.754945, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000026 DAOSIB010000000
VERSION     DAOSIB010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 24372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24372
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2294_length_24372_cov_12.754945"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..2157)
                     /locus_tag="PLD44_03920"
     CDS             complement(13..2157)
                     /locus_tag="PLD44_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HOF98514.1"
                     /translation="MKKKILLSTLIIFSIVCTLNAATLLSPNKNLKLEFQLTPSGEPE
                     YSFFYRGKPIILPSKMGFELKNMPSFVNGFQIHKIDTLTFDQTWEPIWGEVRQIRNHY
                     NEMLVCLEQKSTQRTMKIRFRLFDDGLGFRYEFDKQQNLSYFQIMEEFTQFALTADHK
                     AFWIPGDYDTNEYYYTTTKLSEVDASKVDGSGIGTHGYFAKNAVQSPLMMKTDNGLYI
                     NIFEAALIDYPAMQLLIDQKKLVLTSSLVPSAVGVKAFMQTPQNTPWRTIIVSDKATD
                     ILASKMILNLNEPCKIKDTDFIKPQKFIGIWWAMHVPNKWSWNYAHEPNIKLKDTDWN
                     ALKPLPQHGAKTENVKRYVDFAAANGIPSVLVEGWNVGWEDWMGNWKEEVFDFVTPYP
                     DFDVKEIHRYAASKGVKIIMHHETSASVTNYERRLDKAIEFMKENGYEAVKTGYVGRI
                     IPRGEYHDGQWMVNHYIRVAEKMAENKIMLDAHEPVRPTGLHRTYPNWLACEAARGNE
                     FNAWSAGNPPEHETILPFTRLMGGPMDYTPGIFEIKMSYYQPGNPCQVHTTLAKQLAL
                     YVTMYSPLQMAADLPENYEKRMDAFQFIKDVAVDWDDTKYIEAEPGDYITVARKAKNT
                     DDWFVGAITDENARMAVIPFNFLDAKQWYVATIYSDAENADWKENPMAYKIQKFLVNS
                     KSILKQKLAPGGGAAISLKKATKEEMKELKKL"
     gene            complement(2278..2787)
                     /locus_tag="PLD44_03925"
     CDS             complement(2278..2787)
                     /locus_tag="PLD44_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOF98515.1"
                     /translation="MKTEFFPIVNEKGEIIGKESREKCHSGSFLLHPVVHLHVLNSNN
                     EIYLQKRAKNKDVQPGKWDTSVGGHVDYGETIEEALKREAFEELGIQKFEPEFLYQYI
                     YTSDVESELVYSYFTFYDGIISPSKDEISEGRFWKDEEIEQLMKKGIFTPNFEYEWKK
                     WKEWNPNHK"
     gene            3142..4179
                     /gene="asnA"
                     /locus_tag="PLD44_03930"
     CDS             3142..4179
                     /gene="asnA"
                     /locus_tag="PLD44_03930"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOF98516.1"
                     /translation="MSYFIRPKNYQPLLDLQQTELGIKKIKDFFQSNLSSELRLRRVT
                     APLFVLKDTGINDDLNGVERAVSFPIKDMNDATAEVVHSLAKWKRVALANYGIQNGYG
                     IYTDMNAIRADEELGNLHSLYVDQWDWERVMSREERNLEFLKEIVRRIYATFLRTEYL
                     VWESFPQIEPCLPSDIVFIHAEELRQMYPELTPKERENEFARIHKAIFVIGIGGKLGD
                     GSKHDGRAPDYDDWSTIAENGLPGLNGDIILWNPVLEMAFEVSSMGIRVDSDALLRQL
                     EMEGKQERLQLYFHKSLMEGRLPLSIGGGIGQSRLCMFFLRKAHIGEIQSSIWPEEIQ
                     QECKKLNIVLM"
     gene            4396..4989
                     /gene="hisIE"
                     /locus_tag="PLD44_03935"
     CDS             4396..4989
                     /gene="hisIE"
                     /locus_tag="PLD44_03935"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOF98517.1"
                     /translation="MNLDFQKMGGLIPAIIQDEWTSKVLMLGFMNEEALEKTLATGKV
                     TFFSRTKNRLWTKGEESGNFLNLVSITPDCDNDTLLVKVHPTGPVCHTGNDTCWGETN
                     QGDFSFINYLQNFIKKRYEEMPEGSYTTSLFKSGVNRMAQKIGEEAVETVIEAVNGTD
                     EGFIYEASDLIYHLIVLLTSKGFSLDDIGKELKKRHK"
     gene            4999..5673
                     /locus_tag="PLD44_03940"
     CDS             4999..5673
                     /locus_tag="PLD44_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF98518.1"
                     /translation="MTKKLLVKYVDVNLYQQEMLVLKDVQLEIYSGEFIYLLGKVGSG
                     KSSLLKSFYGEVPIDSGSAGVLNYDLRNLKSNEIPFLRRKIGIVFQDFQLLTDRTVHD
                     NLEFVLKATGWKDKQAIDEQIEAALLQVGMQNKSYKMPHQLSGGEQQRVVIARALLNS
                     PEMILADEPTGNLDPETGNELVQLLYEICRSSDTTVVMATHNMTWVEKYPGRIIRFEN
                     EQLIEN"
     gene            complement(5678..7159)
                     /gene="proS"
                     /locus_tag="PLD44_03945"
     CDS             complement(5678..7159)
                     /gene="proS"
                     /locus_tag="PLD44_03945"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOF98519.1"
                     /translation="MGKELKELTPRSENYSQWYNDLVIKADLAENSSVRGCMVIKPYG
                     YAIWEKIQSELDRKFKETGHVNAYFPLFIPKSYLSREAEHVEGFAKECAVVTHHRLKA
                     DPEGNGLIVDPEAKLEEELIVRPTSETIIWSTYKNWIQSYRDLPILINQWANVVRWEM
                     RTRLFLRTAEFLWQEGHTAHATEAEALEEAKKMLNVYANFVENFMAIPVVKGVKSPNE
                     RFAGALETFCIEALMQDGKALQAGTSHFLGQNFAKAFDVTFVDKNNKQDYVWATSWGV
                     STRLIGALIMAHSDDNGLVLPPTLAPHQVVIVPIYKQPEQLSAITEKAEKIMKELKKF
                     GITVKYDDDDTKKPGWKFAEYELKGVPVRLAIGARDLENNTVEVARRDTLTKETVSAE
                     NIEIYVSKLLEEIQKNIYQKAFDFRTSVTREVNSYEEFKIEIEKGGFLLCHWDGTPET
                     EEKIKQETKATIRCIPLEGDQTPGVCMVTGKPSKQRVIFARAY"
     gene            complement(7287..7697)
                     /locus_tag="PLD44_03950"
     CDS             complement(7287..7697)
                     /locus_tag="PLD44_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOF98520.1"
                     /translation="MIFQTEITLQAYRKGCHLITREILQHLPVLPEKGLLHLFLKHTS
                     AALTINENADPDVQTDLFEIFSRLVPENQSYYEHTLEGSDDMPAHAKSTLSGVSLLIP
                     ITNGHLNLGIWQGIYLCEFKNRGGNRTLVATIIS"
     gene            7989..9281
                     /gene="tyrS"
                     /locus_tag="PLD44_03955"
     CDS             7989..9281
                     /gene="tyrS"
                     /locus_tag="PLD44_03955"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOF98521.1"
                     /translation="MNFIEELRWRGMIHSMMPGTEEQLAREMTTAYVGIDPTADSLHI
                     GHLVSIMILRHFQRAGHKPIALIGGATGMIGDPSMKSSERNLLDEATLRHNQACIKNQ
                     LTKFLDFGSDAPNAAELVNNYDWMKDYTFLNFIRDIGKHITVNYMMAKDSVKKRLSSD
                     SSEGMSFTEFSYQLIQGYDFLWLYENKNCKLQMGGSDQWGNITTGTELIRRKTGGEAF
                     ALVCPLITKADGGKFGKTESGNVWLDARYTSPYKFYQFWMNVSDADAEKYIKIFTFLS
                     KEEIDALIDEHTKAPHLRILQKKLAQEVTIMVHSEADYQAAVEASNILFGNSTPEALR
                     KLDEDTLLAVFEGVPQFNIPKSSLESGIKAVDLFTETAPVFPSKGEMRKMVQSGGVSI
                     NKNKLKQFDEVINTSYLIDNKYLLVQKGKKNYFLLIAE"
     gene            9596..9666
                     /locus_tag="PLD44_03960"
     tRNA            9596..9666
                     /locus_tag="PLD44_03960"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9628..9630,aa:Gln,seq:ctg)
     gene            9907..10608
                     /gene="radC"
                     /locus_tag="PLD44_03965"
     CDS             9907..10608
                     /gene="radC"
                     /locus_tag="PLD44_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOF98522.1"
                     /translation="MEDNDKRLTLREWAEDDRPREKMLRKGIQSLSDAELLAILIRSG
                     SKNETVVELSRRILHECNDNLNELALLSVHDFCQRFKGIGEAKAITVIAALELGKRRK
                     TSEMLERKKITSSKDLLELFEPLLIDLPHEEFWVAFMNSGNKVLETYRLTQGGSKQTV
                     VDVPMLLKMALTKSAHALAVAHNHPSGQNQPSHEDEAITKKIKMGCDAVGITFLDHII
                     VARGKYYSFFDNGKF"
     gene            10675..11595
                     /locus_tag="PLD44_03970"
     CDS             10675..11595
                     /locus_tag="PLD44_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF98523.1"
                     /translation="MPFLEVKNVVKQYAEHLALNNVSLDVPENTIYGLLGPNGAGKTT
                     LIRIINCMTAPDSGTVLLNGKPLTFEDVRHIGYLPEERGLYKKMKVGEQALYFAQLKG
                     MSRHDALKALKYWFQKFEIQSWWNKKVEELSKGMAQKVQFIITILHEPKLLIFDEPFS
                     GFDPVNTDLLKQEILHLKEQGATIIFSTHNMASVEELCENISLIHRSKIVLQGNVAEI
                     RASFSNKTFKVITREPVLDGHYFELVHQTRKNGGVESIIKKVEACNNNILLSEILQQT
                     EILAFEELLPSMNDIFIETVSGHSKNSIEN"
     gene            11632..12945
                     /locus_tag="PLD44_03975"
     CDS             11632..12945
                     /locus_tag="PLD44_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF98524.1"
                     /translation="MSKIHLIIQREYTTRVMKRSFILLTFLTPLFFAAMIILPVWMST
                     LKDEKEKHILVIDQTHQYINALKSNNTYIFDFIDQPLNQVRKTHKKDKKIAAILLITD
                     DLLKNPAAATLYSEEQVNLETKSYIEGLLNEYLREQKLASYNIPDLDKIIQDSNSKIN
                     ISTIRWDEEGKEKQSSAELALIIGMISAFIIYMFILTYGSQVMSGVIQEKTNRVVEII
                     VSSVKPFELMLGKIIGIALVGLTQFFLWIILTFVLLFVAQFFFSNQIDMSMLQQTNQM
                     AAMPAASMENMGNTNILVVLQSFDFVQIGIYFLIYFLGGYLLYASLFAAVGSAVDNET
                     DTQQFSFPLTLPIIFALFVAIYGAQNPDSSLTFWCSLIPFTSPVVMMVRLPYGVAVWE
                     IVLSLAILILSFIATTWVAGKIYRTGILMYGKKTSWKELWKWLNY"
     gene            13023..13925
                     /locus_tag="PLD44_03980"
     CDS             13023..13925
                     /locus_tag="PLD44_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOF98525.1"
                     /translation="MMPPFLKSGDMVQIVSPASAIHPSYVEGAKRTLSEWNLKVLEGK
                     YARSAYGRFAGTKEERIFDLQQALDDPEVKAIFMSRGGYGLVQIIDKINFEQFKKSPK
                     WLIGFSDISILHAAVNRMGIASIHGIMAKHLAELPRTSDAVFHLREILFGRLPVYQLP
                     FHPLNRQGKITAKLLGGNLSVLMGLRGTSFDLPFEDAVLFVEDTGEKPYQVDRMIQNL
                     RIGGIFEKISGLIVGQFTDYESDPLMMQTIEEMFAEAVKNYSYPVCFNFPAGHIDYNL
                     PLLLGENITLEVSAENVLVDFNRQ"
     gene            complement(14035..14463)
                     /locus_tag="PLD44_03985"
     CDS             complement(14035..14463)
                     /locus_tag="PLD44_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98526.1"
                     /translation="MKPTCIAYTNTNEYNIQTCGFSIRPLKNKLYSDEKTILFVLAIF
                     VSCSLMKADPAKKVNVTYQDGKLKVEALHKVRNASNHFIDQVVVKVNGKEMKTIRLDK
                     QTSTASQLVEIELPDLKKGDQIEVVTRCNEFGKKNGKLIL"
     gene            14811..15938
                     /locus_tag="PLD44_03990"
     CDS             14811..15938
                     /locus_tag="PLD44_03990"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HOF98527.1"
                     /translation="MAKFWGRNKATFPLTMLLSYVVSFMQAQTETTKPLQLSVSYTGE
                     EAGNFSGGIKRGSTYLGLVSLKAELNTQYAGWWEGGTFMAEGMNTHGGEPAANLTGDF
                     QGVSNIEAGNHTFLNNLWFAQEIGKIKLTAGLQNMDGNFASTEYGSAFINSSFGIPST
                     FAYNIPSPIFPFTALGLRLEWNILENLWLKTAIYDGQPDDFESHPHRLNWEIGQKEGF
                     LSISEIEWGKSLLKNQNGSYKIGFYYHQSTDSLNIKQNNKGAYLIVDQQISKQTAAFA
                     QLAISPKNKNQHYLYAGAGINLKGILRQNSDDLLGLGVAYAALKNFDKKGETAFELTY
                     QYCFNEHFALQPDLQYILHPAGTEEKINNAFAGLLRFYINF"
     gene            15948..18458
                     /gene="feoB"
                     /locus_tag="PLD44_03995"
     CDS             15948..18458
                     /gene="feoB"
                     /locus_tag="PLD44_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOF98528.1"
                     /translation="MQIKTTVLADLEPGQEAVITKIMGHGSFRRRLTEMGFVKGKKIK
                     VIKKAPLQDPIEYEIMGYKVSLRQSEAELVEVMLMDEAQEWLNNQKFEGTFIDNEEKN
                     IPKEEILKHSLLKKEKVIEVALVGNPNSGKTTLFNYASGAHERVGNYAGVTVDSKEAI
                     LKKNGYVFKIVDLPGTYSINEYTPEELFVRNHLTEKMPDVVINVVDASNLERNLFLTT
                     QLIDMDIKVVMALNMFDELEKKGIHFDYRAFGKMLGIPVVPTIASKGKGIDELFKKVI
                     EVYEDADPISRHIHINYGTDIEKAIKEIQDVIWKEPTITDKFSSRYLAIKLLEGDKTT
                     MSQLEKYNNYKEVIKTAEKVRQELEKEYGETAETIITEAKYGFIDGALKETLKLPKKN
                     RRETTHELDDLLTHKIWGYPIFLFFMWLMFEATFKLGNYPMEWIDNGVTLLGNWIENV
                     MPEGALRDLIVQGIIGGVGGVIIFLPNIMILFFFISIMEDTGYMARASFIMDKLMHKI
                     GLHGKSFVPLVMGFGCNVPAIMATRTLKNRKDRILTMIIIPFMSCSARLPVYLLLISA
                     IFYKHQGLILFSLYLIGIMVAVITALLVKKIAFKKEEAPFVMELPPYRIPTLKNTTLH
                     MWDKSKEYLKKIGNVILLVSIIIWALGYFPHNKSISEHYDTQIAKVQQLPLDKEEKET
                     KILTLEVAKESALQEQSYLGQLGHFIQPAMYPLGFDWKMGVSLLAGLSAKEVVVSTMG
                     ILYQAERGADENSASLQEKIQQQVHTSGPLIGQKVFTPITAFAFMIFILIYFPCIAVI
                     SAIKKEGGWQWAVFSVVYNTSFAWLAAFAVYQIGILIV"
     gene            complement(18803..20251)
                     /locus_tag="PLD44_04000"
     CDS             complement(18803..20251)
                     /locus_tag="PLD44_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOF98529.1"
                     /translation="MQEIKFYKGENIPLELHKVRVVQKLHLVPIERRLEALQEGGYNT
                     FRLSTKDVFLDMLTDSGTNAMSDNQMAGMMQADDAYAGSQSFYRLQKAVQDVLGKKYY
                     LPVHQGRAAENIISYAYVKKGSIIPMNYHFTTAMAHITQYGGKIAELLYDEAYVIDSE
                     HPFKGNMNLEKLEEIIQLHGAENIPFIRMEASTNLIGGQPFSIQNLRDVRSIADKYKI
                     RLVLDASLIGENAFLIKQREEEFKNKSLGEILKIMTELADLVYFSARKLSSSRGGGIC
                     TDSNEIYHELEALVPLFEGFLTYGGMSVREMEAMAVGLYETLDETIICQSPNFIKYLV
                     AALDSIGIPVIKPAGVLGVHLDAMQICAHIPQKEYPAGALAAALFLISGIRGMERGSI
                     SNQRDEYGNETYADMELLRLAVPRRVFTLSQIKYVEDRVNWLYDNRQLIGGLRFVYEP
                     PVLRFFMGGLEPVSDWPQKLMAKFKDDFGESL"
     gene            complement(20558..22189)
                     /locus_tag="PLD44_04005"
     CDS             complement(20558..22189)
                     /locus_tag="PLD44_04005"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOF98530.1"
                     /translation="MAKEFKYQELFQLGKDKTEYYLLSKDFVSTAEFEGKEILKVQPE
                     GLTFLARTAFRDCSFLLRPEHQKQVAAILSDPEASENDKYVALTMLRNAEISAKGILP
                     FCQDTGTATIYAKKGSNVFTDGTDEEALSKGVYETYTQENLRYSQNAPLTMYDEVNSG
                     TNLPAQIDIMASHGNEYEFLFIAKGGGSANKSFLFQETKALLQPDTLEKFLTEKLKLL
                     GTSACPPYHIAFVIGGTSADACMKTVKLAAAKYYDELPTTGNELGRAFRDVELEEKIL
                     KAAQNLGIGAQFGGKYFAHDVRIIRLPRHGASCFVGLAVSCSADRNIKAKINKDGIWL
                     EKLEDNPGKYIPEEYRNTGEGNAVKIDLNRPMNEILSTLTKYPVSTRLSLSGTMVVAR
                     DIAHARIKEMLDAGKDLPQYMKDHPVYYAGPAKTPKGMPSGSFGPTTAGRMDTYVDLF
                     QEHGGSMVMIAKGNRSQQVVDACKKHGGFYLGSIGGPAAILAQNSIKKVECIDFPELG
                     MEAVWKIEVEDFPAFILIDDKGNDFFKLIKPTMTC"
     gene            complement(22293..22895)
                     /locus_tag="PLD44_04010"
     CDS             complement(22293..22895)
                     /locus_tag="PLD44_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HOF98531.1"
                     /translation="MKSSLIILSFFATGLLLGIFRLLPETLFNEEMSFYVLCCLMFTV
                     GFSIGQQGNFVRQFRRLNPRIVFLPLFTIAGTWLGTLAVGFFIDGFSLFDSLAASSGF
                     GYYSLSSILITRYKGVELGTIALLSNIVREIFTLLFAPLMVKYFGKLSPISSGGATTM
                     DTTFPVIVQSAGKDFALVSIYHGFIMDFSVPFLVTFWCSL"
     gene            complement(22885..23166)
                     /locus_tag="PLD44_04015"
     CDS             complement(22885..23166)
                     /locus_tag="PLD44_04015"
                     /inference="COORDINATES: protein motif:HMM:NF015889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015661 - L-lysine efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903401 - L-lysine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysO family transporter"
                     /protein_id="HOF98532.1"
                     /translation="MFTVLGCMLAGMVVGFIFRKKHFKIIQSVLFVLIWLLLFLLGAE
                     IGSNPAVIRQTGKLGFDALLIGTAGTLGSILGAAFLWKWIKPDKSTHEK"
     gene            complement(23312..24202)
                     /locus_tag="PLD44_04020"
     CDS             complement(23312..24202)
                     /locus_tag="PLD44_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOF98533.1"
                     /translation="MEHHHQHTHTHGESNIKMAFFLNFGFTVFEIFGGIFTNSIAIIS
                     DAIHDMGDSLSLGLAWYFQKKSTKESDKNYTYGYKRFSLLGAIINSIILITGSIFVLY
                     EAIPRLLHPETTKTQGMIVFAVIGIVVNGLAVLRLKKGSSINEKVVSLHMLEDVLGWA
                     AILVGSLIMYFFDLPVIDPVLSVLITIYILFHVFRNVKESLHILLQGSPTDVDLEKIS
                     QLLLSIENVDEIHDLHIWSIDGNYNILTVHVVLKQPVTIEKAVEIKNKIRSELLQQGI
                     EHATIEFETTEENCSLQQCC"
BASE COUNT         7480 a         4630 c         4697 g         7565 t
ORIGIN      
        1 aaagaaaata gtttataatt ttttcaattc tttcatttct tcttttgtag ctttcttcag
       61 actaatggct gcaccaccac cgggagcaag tttttgtttt aatatgcttt ttgaattgac
      121 cagaaacttt tgaattttat aagccatcgg attctctttc cagtcggcat tttcagcatc
      181 actataaatt gtggcaacat accattgttt tgcatctaaa aagttaaaag gaataacggc
      241 cattcgtgca ttttcatccg ttatggctcc cacaaaccag tcatcggtgt ttttagcttt
      301 tcgcgctacc gtgatgtaat cgcccggttc ggcttcaatg tattttgtgt cgtcccaatc
      361 gacagccaca tctttgataa attgaaaagc atccattctt ttttcgtaat tttcgggtaa
      421 atcggctgcc atttgtagtg gactgtacat tgttacataa agagccaatt gttttgccag
      481 tgttgtatgc acttgacagg gattacccgg ctgataataa ctcattttta tttcaaaaat
      541 tccgggtgta taatccattg gacctcccat cagacgggta aaaggaagaa tagtttcatg
      601 ttcgggcgga ttgccggcac tccaagcatt aaattcattt cctctggctg cctcgcaggc
      661 taaccagttg ggataggttc ggtgcaatcc ggttggtcgt acaggttcgt gagcatcaag
      721 cataattttg ttttctgcca ttttttcggc tactctgata tagtgattca ccatccattg
      781 tccatcgtga tattcaccac gaggaataat tctgcccaca taaccggttt taactgcttc
      841 gtaaccgttt tctttcataa attctatggc tttgtccaat cgccgttcat aattggttac
      901 cgaggccgaa gtttcgtgat gcataataat tttaactcct ttggaagcag cgtatcgatg
      961 aatttccttc acatcaaaat ctggataggg agtaacaaaa tcaaaaactt cttctttcca
     1021 gtttcccatc caatcttccc aacccacatt ccatccttcg accaacacac ttggtatgcc
     1081 gttggctgct gcaaaatcaa catacctttt tacgttttcg gttttggctc cgtgttgtgg
     1141 aaggggtttt aaagcattcc aatcggtgtc tttcagtttt atgtttggct catgagcata
     1201 attccacgac catttattgg gaacatgcat ggcccaccaa atgccaataa acttttgcgg
     1261 ttttataaaa tcggtatctt taattttaca aggttcattc agattcaaaa tcatttttga
     1321 agccagtata tcggttgctt tatcgctgac aataatggta cgccagggcg tattctgagg
     1381 agtctgcata aaagctttta ctcctacagc actgggaaca agcgaagaag tcaataccag
     1441 ctttttttga tcgataagca gctgcatggc cggataatcg atcaaggcag cttcaaaaat
     1501 attgatatac aatccgttat ctgttttcat catcaaagga ctttgcacgg cgtttttggc
     1561 aaaatatccg tgtgttccta ttccagatcc atcaactttt gaagcgtcaa cttccgaaag
     1621 ttttgtcgtc gtataataat attcgttggt atcatagtcg cccggtatcc aaaatgcctt
     1681 gtggtcagcc gtcaacgcaa attgtgtaaa ttcctccata atttgaaaat aactcaaatt
     1741 ctgctgtttg tcgaattcgt atcggaatcc caaaccatcg tcaaacaaac gaaaacgaat
     1801 tttcatggtt cgttgagtgg acttttgttc gagacaaacc aacatttcgt tgtaatgatt
     1861 tctgatttga cgaacttctc cccaaattgg ttcccatgtt tggtcaaaag tgagggtgtc
     1921 tattttatga atttgaaaac catttacgaa agacggcata ttttttagtt caaaacccat
     1981 ttttgacggt agaattatcg gttttcccct gtagaagaaa ctgtattcgg gttcaccgga
     2041 tggcgtcaat tgaaactcga gcttcaaatt tttgttggga gaaagtagtg tagctgcatt
     2101 taatgtgcag acaatcgaaa aaataatcag tgttgaaagc aaaattttct ttttcatgtg
     2161 ttgaataaat ttaaaaaaat aattgtatga ataaacaaaa ttaacggttt tttttgaaga
     2221 tttatccatt aggaattaaa attttacaaa catcgattgc aaacgtttga agaaaagtta
     2281 tttgtgattt gggttccatt ctttccactt tttccattca tattcaaaat ttggtgtaaa
     2341 tatgcctttc ttcatcagtt gttcaatttc ttcatctttc caaaatcgtc cttcacttat
     2401 ctcatctttc gatggggaaa taattccgtc gtaaaaagtg aaataactgt aaactaattc
     2461 cgactcaaca tccgaagtgt aaatgtattg ataaagaaat tcaggctcaa atttttggat
     2521 gcccaattct tcaaaggctt ctcttttcaa tgcctcttct attgtttctc cataatccac
     2581 atgtccgcct accgaagtgt cccatttccc gggttgcacg tctttgtttt tagctctttt
     2641 ttggaggtaa atttcattat tggaatttaa aacgtgcaga tggacaaccg gatgcaataa
     2701 aaaagaaccg ctatgacatt tttccctgct ttccttcccg ataatttctc ctttttcgtt
     2761 cactattgga aaaaattccg ttttcatttt caatcgcttt ttggatgaat tttcaaagtt
     2821 aatcaaattt attgtatttg atagttttgt tcataaaaca gcaaattgta tatctatttt
     2881 ttatcaatac tatgctttta ctgtaccatt gtaaataaaa atgtcgataa ttgatatatt
     2941 ttgatttttt aacatctcaa tttggttaaa aattttattt tcaatttact gattttcaat
     3001 ggattattat ttattccgaa aatttttact cttcctttcc ttcttgtgaa tcaaaaaaat
     3061 cggtaaggaa tgattatctt tgcttcttaa ttttttaaaa caatagaaca ttttatcgac
     3121 catcagcaaa taaattaaaa tatgagctat tttattcgac caaaaaatta tcaaccttta
     3181 ctcgatttgc agcaaaccga attgggcatc aagaaaataa aagatttttt tcagtcgaat
     3241 ctctcctccg aattgcgttt gcgccgtgta actgcacctc tttttgtcct caaggatacg
     3301 ggaattaatg acgatttaaa cggtgtggaa cgcgctgtaa gttttcccat caaagatatg
     3361 aacgatgcca ctgccgaagt tgttcattcg ttggcaaaat ggaaaagggt ggcgttggcc
     3421 aattacggta tccaaaacgg atacggtatt tacaccgata tgaatgccat tcgggcagac
     3481 gaagagcttg gaaatctcca ctctttatat gtcgatcagt gggactggga aagagtcatg
     3541 agccgcgaag aaagaaattt ggaatttcta aaagagattg tccgtcgtat ttatgccact
     3601 tttttgcgga cagaatatct ggtttgggaa agttttcctc aaattgaacc ttgtttgcct
     3661 tccgatatag tatttattca tgcggaagag cttcggcaaa tgtaccctga acttactcca
     3721 aaggaaagag aaaacgagtt tgccagaata cacaaagcta tttttgtcat tggcataggg
     3781 ggcaagttgg gcgatgggag caaacatgat ggacgtgctc ccgattatga tgactggtcg
     3841 acgattgccg aaaatggctt gcccggactt aatggcgata ttattctttg gaatccggtt
     3901 ctcgaaatgg cttttgaagt ttcatcaatg ggcattcgag tggacagcga cgcattattg
     3961 cgtcagttgg aaatggaagg aaagcaggaa agattgcaat tgtatttcca caaatcactc
     4021 atggaagggc gtctgcctct ttccattggc ggaggtatcg gacagtcgcg actttgtatg
     4081 tttttccttc gtaaagcgca tattggcgaa attcagtcaa gtatctggcc cgaagaaatt
     4141 caacaggaat gtaaaaaact aaatattgtt ttaatgtaat cggtttccaa ttatttcaac
     4201 caaataaaat tttcccagaa taccctctgg tttttgggat tttttgtgcc aataaatatt
     4261 gggaagcgaa taaattttta acgacaaatg aagcgaaatt cttacaaata atcttttttg
     4321 ccgatttttc tattgaatat gggcattttt ttgtactttc gcttgctgta aattcaaaat
     4381 aaaatttttg acaatatgaa tttagatttt caaaaaatgg gaggactgat tccggccatt
     4441 attcaagatg agtggacatc caaagtcttg atgctgggat ttatgaatga agaagcactc
     4501 gaaaaaactt tggctacagg aaaagttact tttttcagcc gaaccaaaaa tcgtttgtgg
     4561 acaaaaggag aggaaagcgg gaatttttta aaccttgttt ccattacgcc cgattgcgac
     4621 aacgatactt tgctcgtcaa agttcatccc accggccctg tttgccatac gggtaatgac
     4681 acttgttggg gagaaaccaa tcagggagat tttagtttta taaattattt gcagaatttt
     4741 atcaagaaaa gatacgaaga aatgcccgaa ggttcttata cgacttctct atttaaaagt
     4801 ggtgtgaacc gaatggctca aaaaattggc gaggaagctg tcgaaacagt gattgaagca
     4861 gttaacggaa cggatgaagg ttttatttat gaagcttccg atttaattta tcatcttatt
     4921 gttttgctga cttcaaaggg attttcgctc gacgatattg gaaaagaatt gaaaaaacga
     4981 cacaaataag tgaaaatgat gacaaaaaaa ttattggtca aatatgtcga tgtaaatctc
     5041 tatcagcagg aaatgttggt gttgaaggat gtgcagctcg aaatttattc cggcgaattt
     5101 atttatttgt tgggaaaagt tggaagtggg aaaagttctt tgctgaaatc gttttatgga
     5161 gaagtaccta tcgattcagg ttcagcagga gttttaaact atgatttaag aaatttaaaa
     5221 tctaatgaaa ttcctttttt gaggcgaaaa atcggcatcg tttttcagga ttttcaactg
     5281 ctcaccgatc gaaccgtaca cgataacctt gaatttgtat tgaaggctac cggttggaag
     5341 gacaaacagg ccatagatga acagattgaa gcggctttgt tgcaggttgg gatgcagaac
     5401 aaaagttaca aaatgcctca tcaactttcc ggcggcgaac agcaacgtgt agttattgct
     5461 cgggctttac tcaattcacc cgaaatgatt ttggctgatg aaccgactgg taatctcgat
     5521 cccgaaaccg gcaatgaatt ggttcaactg ctttacgaaa tttgtcgctc gtcagatacc
     5581 acagtagtaa tggccaccca caacatgact tgggtagaaa aatatcccgg acgtattata
     5641 cgtttcgaaa acgaacaatt gatagaaaac tgatttttca gtaagctcgg gcaaaaatca
     5701 ctctttgttt ggatggtttg cctgttacca tacacactcc cggagtttgg tctccttcca
     5761 gaggaataca ccgaatggta gctttggttt cttgcttgat tttttcttcg gtttccggtg
     5821 taccatccca atgacaaagt aaaaatccac ctttttctat ttcaatctta aattcttcgt
     5881 acgaatttac ttctctggta accgaagttc tgaaatcaaa agctttttga taaatatttt
     5941 tctgaatttc ctccagaagt ttggaaacat atatctcaat attttcggcc gaaacagttt
     6001 cctttgttaa agtatcccga cgtgccactt caaccgtatt gttttccaaa tcacgtgcgc
     6061 cgatagccaa tcttactgga acgcctttca gttcgtattc ggcaaatttc caacccggtt
     6121 ttttggtatc gtcatcatca tatttcactg taattccgaa ctttttcaac tccttcataa
     6181 ttttttctgc cttttcggta attgcagata gctgttcggg ttgtttgtaa ataggaacaa
     6241 tgactacttg atgtggtgcc agcgttggcg gcaaaaccag cccgttgtcg tcggagtgag
     6301 ccatgataag cgctccgata agtctggttg aaactcccca agaagttgcc catacataat
     6361 cctgcttgtt gtttttatcg acaaatgtaa cgtcaaatgc tttcgcaaaa ttttgtccca
     6421 gaaagtggga agtacctgct tgaagagctt ttccatcttg catgagtgcc tcaatacaaa
     6481 atgtttccaa agctccggca aaacgttcat tgggcgattt cacgcctttc accactggaa
     6541 ttgccatgaa attttcaaca aagttggcat aaacattcaa catttttttt gcttcttcca
     6601 gtgcttctgc ttccgttgca tgagccgtat gaccttcttg ccacaaaaac tcagcggtac
     6661 gcaaaaacaa tcttgtgcgc atttcccagc gtactacatt tgcccattga ttgatcaata
     6721 tcggcaaatc acggtacgat tgaatccagt ttttgtaagt actccaaatt atggtttcgg
     6781 aagtgggtct tacaatcagt tcttcttcca gcttagcttc aggatccaca atcagtccat
     6841 ttccttcagg atcagctttc agacgatggt gcgtaaccac tgcacattct ttggcaaaac
     6901 cttccacgtg ttcggcttcc cggctcaaat aggatttggg aataaacagc ggaaaataag
     6961 cattgacatg ccctgtttct ttgaattttc tgtcgagttc cgattgtatc ttttcccaaa
     7021 tagcatagcc gtagggttta ataaccatac aacctcttac tgaagaattt tctgccaaat
     7081 cggcttttat caccaaatca ttgtaccact gtgagtagtt ttccgaacgt ggtgtcaatt
     7141 ctttaagttc ttttcccata atttgcgtat aatgatattt ctttttgaaa aggaaacgat
     7201 ctgtaatttg taggattgtt tcccgaaaaa atttcggagt gcaaagttaa tcaaaattta
     7261 cgacttattt gcttgctgaa tcaggtttaa ctgattatgg tggctaccaa ggtacgattt
     7321 ccgcctctgt ttttgaattc gcaaagataa attccctgcc atattccaag attcaaatgt
     7381 ccattggtaa tggggataag cagacttact ccggaaaggg tgcttttggc atgtgcaggc
     7441 atatcatcgc tgccttccag tgtatgttcg tagtaagatt gattttccgg caccagtcgg
     7501 ctaaaaattt caaacagatc cgtttgtaca tcaggatcgg cattttcgtt tatggttaag
     7561 gcggcagaag tatgtttcag aaaaagatgc agcaatcctt tttcgggcag gacaggcaaa
     7621 tgttgtaaaa tttcgcgtgt aattaaatgg cagccctttc ggtaagcttg aagtgttatc
     7681 tcagtttgaa atatcattgt ttgaatattt ttcgacaaaa ataactcttc ttaaaaattt
     7741 gtgcaataaa ccgacaaaga gaaaatttga ttacttgatt tcgtcaacac atttttttac
     7801 aaaagggaag cggttgtagt cgttgcgcat taaattgtcg ttccagaaaa tcaaatcaat
     7861 atcaatatca ataactcaaa gtaaaatttt attttaccat tgtttttttc gagttttcat
     7921 aggtctttgg gtaaattgaa aataaacttg taaatttgca ctccgaatta aaaaacctga
     7981 atttgcacat gaattttatt gaagaattac gttggcgggg aatgattcat tccatgatgc
     8041 ccggcacgga agaacaactt gccagagaaa tgactacagc ttatgtgggt atcgatccaa
     8101 cagccgattc gctccatatt ggtcatttgg tcagtattat gattttgcgt cattttcaac
     8161 gtgcaggcca taaacccatc gctctgatag gtggcgctac aggaatgatt ggagatcctt
     8221 ccatgaaatc atccgaacga aatctgttgg acgaagctac cttgcgacac aatcaggcgt
     8281 gcattaagaa tcagttgaca aaatttctcg atttcggcag cgatgcgccc aatgccgctg
     8341 aattggtcaa caattacgat tggatgaaag attatacctt tcttaatttt attcgcgata
     8401 ttggcaagca catcaccgtt aattacatga tggcgaaaga ttccgtaaaa aaacgtttaa
     8461 gcagcgattc gagcgaagga atgtctttta ccgaattttc ctatcagttg attcagggtt
     8521 acgatttttt gtggctttac gaaaataaaa attgtaaact tcagatggga ggttccgatc
     8581 aatggggaaa cattactacc ggaaccgaac tgattcgccg aaaaactggt ggcgaagcct
     8641 tcgctttggt ttgcccgctg attaccaaag ccgatggtgg caaatttggt aaaacggaaa
     8701 gcggaaatgt ttggcttgat gctcgttata cttctcctta caaattttat cagttttgga
     8761 tgaatgtaag cgatgccgat gccgagaaat atattaaaat atttactttt ctgagtaagg
     8821 aagaaatcga tgcactgatc gacgaacata ccaaagcgcc tcatttgcga attttacaaa
     8881 aaaaactggc gcaagaagtt accatcatgg tacattctga ggctgattat caggctgccg
     8941 ttgaagcttc caatatttta tttggaaatt ctacgcctga agctttgaga aaactcgacg
     9001 aggatacatt gctggccgtt tttgaaggag ttccgcaatt caacattccg aaatcttctc
     9061 tcgaaagcgg aataaaggcg gtcgatcttt ttacggaaac cgcacccgtt tttccttcaa
     9121 agggagaaat gagaaaaatg gtacaatcgg gaggagtaag cataaataaa aataaactga
     9181 aacagtttga cgaagttatc aacacttctt accttatcga caataaatat ttactggttc
     9241 agaaagggaa gaaaaattat tttctgctga tagccgaata gaaaatcgca ctacttcaac
     9301 atattttggc tgtaaaattt tgacagaaaa aaaatcggtt gtatctttgt gcccgcttgc
     9361 tgaaagattg cagcgaaagt ttgtttgtct catggtgtaa tggtagcact gcagtttttg
     9421 gtactgcctg tctaggttcg aatcctagtg agacaactgt ttttctggcg gttgagcttt
     9481 ttcaaaaggt tcaatcgttt tttttacaag ccaaaaaaac tctttgccac aaacaaaata
     9541 atttgtattt ttgcaaaggg ttttaaagtt cccgatttct ctgaaataca cattctgtct
     9601 catggtgtaa tggtagcacg tcagattctg gatctgcctg tctaggttcg aatcctagtg
     9661 agacaactac ttcccaacct gatttctcct ttttttctgt atcgattcac aagataatct
     9721 ttccgtattg tttgcaagtt tactaaactg atggtatttt tatgcccgaa aaaatttatg
     9781 aaccttttta ttgttcaaaa caatttattc cgacaataaa agtattttcg taaatccata
     9841 accaatcgtc ctgctttaat tttttattcc ttttttcata gatttgtttg caaaacattt
     9901 tataaaatgg aagacaatga caagcgtttg acacttcggg aatgggcaga agatgatcgc
     9961 ccacgtgaaa aaatgctgcg gaaaggtatt caaagtcttt ccgatgccga attgctggct
    10021 attctcattc gctcgggcag caaaaatgaa accgtcgttg aactttctcg ccgtattttg
    10081 catgaatgta acgataattt gaatgaattg gcgctgttga gtgttcatga tttttgtcag
    10141 cgttttaagg gaattggcga agcaaaagcc attacggtca ttgctgctct tgaactgggc
    10201 aaacgccgaa aaaccagtga aatgttggaa cgtaagaaaa ttacttccag caaggattta
    10261 ctggaacttt ttgaaccttt actgattgat ttgccacacg aagaattttg ggttgcattt
    10321 atgaacagtg ggaacaaagt tttggaaact tatcgtctga cgcaaggtgg cagcaagcaa
    10381 acagttgtgg atgttcccat gttgctgaaa atggcactta caaaatcggc tcatgctctt
    10441 gctgtggcac acaatcatcc ttcgggacaa aatcaaccca gccatgaaga cgaagctatt
    10501 accaaaaaaa tcaagatggg atgcgatgcg gttggaatta cttttttaga tcacatcatc
    10561 gttgctcgtg gaaaatacta cagttttttt gataatggaa aattctaagt cattttgccg
    10621 aaaaatgaaa attatttctt ctaaaaatct ataaaaacaa ttttgtaaag aattatgcct
    10681 tttttggaag ttaaaaatgt tgtcaaacaa tatgcagagc atctggcttt aaataatgtg
    10741 agtctggacg ttcccgaaaa taccatttat ggcttacttg gcccgaacgg tgcaggaaaa
    10801 accacactga tacgtatcat taactgcatg actgctcccg atagtggaac agtgcttttg
    10861 aacggaaaac ctctgacgtt tgaagatgtg cgtcatattg gatatttgcc tgaagaacgc
    10921 ggactttaca agaaaatgaa agttggtgaa caggcgcttt attttgccca actgaaagga
    10981 atgtcgcgac acgatgccct taaagcttta aaatactggt ttcaaaaatt tgaaattcaa
    11041 tcgtggtgga acaaaaaagt ggaagaactt tccaaaggaa tggcacagaa agttcaattc
    11101 attatcacca ttcttcacga acccaaattg ctgatttttg acgaaccatt cagcggattc
    11161 gatccggtga acaccgattt gctgaaacag gaaattcttc acttgaaaga acagggagcc
    11221 accattattt tttctacgca caatatggct tcagtggaag aactttgtga aaatatttca
    11281 ctcattcatc ggtcgaaaat tgttttgcag ggaaatgttg ccgaaattcg ggcttcgttt
    11341 tctaacaaaa ctttcaaagt gataaccaga gaacccgttt tggatggtca ttattttgaa
    11401 ttggtacatc aaactcggaa aaacggtggg gtagaaagta tcataaaaaa agtggaggct
    11461 tgcaataaca acatcctttt gtccgaaatt ttacaacaaa ccgaaatttt ggcttttgaa
    11521 gaattattgc ccagcatgaa cgatattttt atagaaaccg tcagtgggca ctcgaaaaat
    11581 tcaattgaga attagtgtca taataacttt caaatcaaac aaattttcag catgtctaag
    11641 attcatctca ttattcaaag agaatatact acccgggtga tgaaacgttc atttattttg
    11701 ttgacgtttt taactccctt gttttttgct gccatgatta ttctgcctgt ttggatgtcc
    11761 accttgaagg atgaaaagga aaaacatatt ttggttattg atcaaaccca tcaatacatc
    11821 aatgccttga aaagtaacaa tacatatatt tttgatttca tcgatcaacc actcaatcaa
    11881 gtgcgtaaga cacataaaaa ggacaaaaaa attgctgcca ttttactgat taccgatgat
    11941 ttgttgaaaa atccggctgc cgcaacactt tactccgaag aacaagtgaa tctggaaaca
    12001 aaaagttaca ttgaaggttt gctgaatgaa tatcttcgcg aacagaaact ggcttcgtac
    12061 aatattccgg atttggataa aataattcag gattccaatt ctaagataaa catttcgacc
    12121 attcgttggg atgaggaagg gaaggaaaaa caaagttcgg ccgaattggc gctgattatc
    12181 ggaatgattt ctgccttcat catttacatg tttattctta cctacggttc gcaagtgatg
    12241 agcggcgtta ttcaggagaa aaccaatcgt gtggtagaaa ttattgtttc ttcggtcaaa
    12301 ccttttgaac tcatgttggg aaaaattatt ggcattgcat tagttggcct cacacaattt
    12361 tttctttgga ttattcttac tttcgttttg ctgtttgtag ctcaattttt cttttccaat
    12421 caaatcgaca tgagtatgct tcagcagaca aatcagatgg ctgccatgcc tgctgcttca
    12481 atggaaaata tgggtaacac aaatatattg gtagttctcc aaagtttcga tttcgtacaa
    12541 atcggcatat attttcttat ctattttctg ggtggctatt tgctttatgc ttcgcttttc
    12601 gctgctgttg gttcggcagt ggacaacgaa accgatacgc aacaatttag tttcccgttg
    12661 actttgccta ttatttttgc tttgtttgta gccatttatg gagctcaaaa tcctgattcc
    12721 tccttgacgt tctggtgttc gctgattccc tttacttcgc cggtagttat gatggttcgt
    12781 ttgccttatg gcgtggccgt gtgggaaatt gttctttcgc tggcaattct gattctttcc
    12841 ttcatcgcta ccacttgggt ggcagggaaa atctacagaa caggaatttt aatgtatggt
    12901 aaaaaaactt catggaaaga actttggaaa tggttgaact attgattatt catacgtcag
    12961 attcctgtgg tttaattagt ttgtgatggt tcaattattg ataaaaaatc tcattattgc
    13021 ttatgatgcc tccttttttg aaatcgggag atatggttca aattgtttct ccggcaagcg
    13081 ctatccatcc ttcctacgtt gaaggagcaa aaagaacttt aagtgaatgg aatttgaaag
    13141 tcctcgaggg gaaatatgca cgttctgcct atggtcgctt tgccggaaca aaagaggaaa
    13201 gaatctttga tttgcaacag gcgcttgatg atccggaggt aaaagccatt ttcatgagtc
    13261 gcggtggtta tgggctggtg caaattatcg acaaaatcaa ttttgagcaa tttaaaaaat
    13321 cacctaaatg gttgatcgga ttcagcgata tttccatctt gcatgcagca gtaaacagga
    13381 tgggtattgc ttccattcac ggtataatgg ccaaacatct tgccgaattg cccagaacct
    13441 ctgatgcagt gtttcatttg agagaaattt tgttcgggag attgcctgtt tatcaacttc
    13501 cttttcatcc actcaatcgt cagggaaaaa ttaccgccaa actgctgggt ggaaatttgt
    13561 cggttttaat gggattgcgc ggtacttctt tcgatttgcc ctttgaggat gccgttttgt
    13621 ttgttgaaga tacaggagaa aagccttacc aagtcgacag aatgattcaa aacttacgca
    13681 taggtggaat ttttgaaaaa atttccggtc tgattgttgg ccaatttacc gattatgagt
    13741 ccgatccatt gatgatgcaa accatcgaag aaatgtttgc cgaagctgta aaaaactatt
    13801 cttatccggt ttgttttaat tttcctgccg gccacatcga ttacaattta ccgcttttat
    13861 tgggtgaaaa cataactttg gaagtgtctg cagaaaatgt gctggtcgat ttcaatagac
    13921 aataaaaacc ttgtgtttcg gtagaatcgg ataaagcaac actcaacaaa ttgttgaaca
    13981 gaatctattc aaatttgttg agtgcccatt taacaccaaa acacatacaa aatgtcataa
    14041 aataagttta ccgttttttt taccaaattc gttgcagcgg gttacaactt caatttgatc
    14101 cccttttttt aggtcaggaa gttcgatttc tactaattga gatgctgttg aggtttgttt
    14161 gtccaaccgg atcgttttca tttcttttcc gtttactttc acaaccacct gatcaataaa
    14221 atgattgctg gcatttctaa ctttatgcaa agcttctact tttagctttc catcttgata
    14281 agttacattg actttttttg ccggatcggc cttcatcaat gaacaggaaa caaatattgc
    14341 caatacaaat aaaattgttt tttcatcaga atataattta ttttttaaag gtctgatgga
    14401 gaacccacat gtctgaatat tatattcatt cgtgtttgtg tatgcaatac atgtgggttt
    14461 catatctcca tcaattttta taatttataa aatgtttggt tttgtaagtg ctgaaaaatc
    14521 tccaacctac ttaaagaaat aatttatgcg agttagcctg cgagtcagca gttgaaactg
    14581 attctcataa attattcaaa tcaatatcaa gttgtctaaa aatggttaat gaattgatat
    14641 ttgaatgatt attaggaaat ttcaaattta ttgaaatttt tttggatatt attatttaga
    14701 atcaatctaa attgcgtaac tttgcgtctc caaaacaaga gtagaaacag aaagaaattt
    14761 tattaagatt aatgggaaaa aattcagacc aaaatcggat acaatggtta atggcaaaat
    14821 tttggggtag aaataaggca actttcccgc tgacaatgct gctgagttat gtcgtttcat
    14881 ttatgcaggc tcaaaccgag acgacaaaac ctttgcagtt atcggtttca tatacgggag
    14941 aagaagcagg aaatttcagt ggaggaataa agcgtggcag cacgtatctc ggactggtat
    15001 cgctgaaagc tgaattgaat acacagtacg ctggttggtg ggaaggcgga acctttatgg
    15061 cagaaggaat gaatacgcat ggcggagagc cagcagcaaa tttgaccggc gactttcagg
    15121 gcgtttcgaa tattgaagcc gggaatcata cctttttgaa caatctttgg tttgctcagg
    15181 aaataggaaa aattaaactc actgccggac tgcaaaatat ggatggaaac tttgcctcca
    15241 ccgaatatgg cagtgctttc atcaacagtt cttttggcat tccttctact tttgcttaca
    15301 atattccaag tcccattttc ccatttacag cactcggact ccgcttggaa tggaatattt
    15361 tggaaaattt atggttgaaa acagccattt acgatggcca acctgatgat ttcgaatccc
    15421 atcctcaccg tttgaattgg gaaataggtc aaaaagaagg attcctttct atcagtgaaa
    15481 tagaatgggg aaaaagtctg ctaaaaaatc aaaatggttc ttacaaaatt ggattttatt
    15541 atcatcagtc aacagacagt ttaaacataa aacaaaacaa caaaggcgca tatctgattg
    15601 tcgatcagca aatcagtaag caaactgcag cttttgctca attggcaatc agcccaaaaa
    15661 ataaaaatca acattacctg tatgccggag ccggaatcaa cctgaaagga attttaagac
    15721 aaaattccga cgatttattg ggattaggag ttgcttatgc agcattgaaa aatttcgaca
    15781 aaaagggaga aacagctttt gaactcactt atcagtactg tttcaacgaa catttcgctc
    15841 tccagcccga cttgcaatat atacttcatc cggcaggcac ggaagaaaaa ataaataatg
    15901 cttttgctgg attgctgcgt ttttatatta atttttaaac actcgtcatg caaataaaaa
    15961 caaccgtatt ggctgatttg gaacccggtc aggaagccgt gatcacaaaa ataatgggac
    16021 atggatcatt ccgaagacga ctgacagaaa tgggttttgt caaagggaaa aaaataaagg
    16081 ttatcaaaaa agcacccttg caggatccca ttgagtacga aataatgggt tacaaggttt
    16141 ctttgcgtca atcggaagcc gaattggttg aagtaatgct gatggacgaa gcgcaagaat
    16201 ggttgaacaa tcaaaaattt gaagggactt tcattgataa cgaagaaaaa aatattccta
    16261 aagaagaaat tctcaaacat tcgctactta aaaaggaaaa agtgattgaa gtggctttgg
    16321 tagggaatcc aaattcagga aaaacgacac ttttcaatta cgcatccgga gcacacgaac
    16381 gagtgggaaa ttatgccgga gtaactgtag attcaaaaga agccatactg aaaaaaaacg
    16441 gatatgtttt taaaattgtt gatctgcctg ggacttattc catcaacgaa tatacgcctg
    16501 aagaactttt cgtaagaaat catctcaccg aaaagatgcc cgacgtcgtg atcaacgttg
    16561 tggatgcttc taatctggaa agaaatcttt ttctcactac ccaacttatc gacatggaca
    16621 taaaagtggt aatggcgctc aacatgtttg acgaactgga gaaaaaagga atacatttcg
    16681 actaccgcgc attcgggaaa atgctgggaa ttcctgtggt gccaaccatc gcatccaaag
    16741 gaaaaggcat tgatgaatta tttaaaaagg taatagaagt ttacgaagat gctgatccca
    16801 tttcccgaca cattcacatc aattatggta cagacattga aaaagctatc aaggaaattc
    16861 aggatgtaat ttggaaagag cccaccataa ccgacaaatt ctcttcacga tacttggcta
    16921 tcaaattact ggagggtgat aaaaccacta tgtcccaact cgaaaaatac aacaattaca
    16981 aggaagtaat aaaaactgca gaaaaagtaa gacaagaact tgaaaaagaa tacggagaaa
    17041 cggcagaaac cataattact gaagcaaaat acggatttat tgacggagcg ttgaaagaaa
    17101 cgctcaaact tcccaaaaaa aatcgacgag aaactacgca cgaactggat gacctgctga
    17161 ctcacaaaat atggggctat cccatttttc tattcttcat gtggttgatg tttgaagcca
    17221 ctttcaaact gggaaactat ccaatggaat ggatcgacaa cggggtcacg ctgctgggaa
    17281 actggataga aaatgttatg cccgaaggag ccttgcggga tttgattgtt cagggtatca
    17341 ttggtggtgt gggtggtgta atcatatttc ttcccaacat tatgattttg tttttcttta
    17401 tatcgattat ggaggacacc ggttatatgg ccagagcatc ttttattatg gataaactca
    17461 tgcacaaaat cgggttacac gggaaatcgt ttgttccgct ggtaatgggt tttggctgca
    17521 atgtcccggc cattatggct acccgaacgc tgaaaaacag aaaagaccga attttaacca
    17581 tgattattat tcctttcatg tcgtgcagcg cccgactacc ggtttatctg ctgctcattt
    17641 cggctatttt ttacaaacat caaggcttga tcctgttttc gctttacttg attggaatca
    17701 tggtagccgt cattacagca ttgttggtaa agaaaattgc ttttaaaaaa gaagaagcgc
    17761 cttttgtgat ggaattgcct ccttaccgca tacccacatt aaaaaacacc accctgcaca
    17821 tgtgggacaa aagtaaagaa tacctgaaaa aaataggtaa cgtcatttta cttgtttcca
    17881 tcattatttg ggcgttggga tattttcctc acaacaaatc gatcagcgaa cattacgata
    17941 cacaaattgc aaaagttcaa caacttcctc ttgataaaga agaaaaagaa acaaaaattt
    18001 tgactttgga agtagcaaaa gaatcagcct tgcaggaaca atcttatctc ggtcagttgg
    18061 ggcattttat tcagccggcc atgtatccgc tgggtttcga ctggaaaatg ggcgtcagtt
    18121 tactggccgg attatcagca aaagaggtag tggtgagcac aatgggaatt ttgtatcaag
    18181 ccgaaagagg agccgatgaa aattctgctt cgcttcagga aaagattcaa caacaggtgc
    18241 atacttcagg accgttgata ggtcaaaaag tttttacgcc cataacggca tttgccttta
    18301 tgatttttat cctgatatat ttcccctgta ttgctgtcat atcggctatt aaaaaagaag
    18361 gtggctggca atgggcagta ttctccgttg tgtacaatac ttcttttgcc tggctggcgg
    18421 cttttgcagt ttaccaaatt ggaatcctga ttgtatgata ttaataaaaa cgtaatttta
    18481 taatttattc atgccatgac acaaaccatt ataacctacc taattattgt aggagcgata
    18541 gtaatttcgg ccttaaaaat agtaaaaaca attcgcagca aaaagaattc tccctgtagt
    18601 ggatgcaacg gctgcgaaat aaaacaagaa attctgaaaa acagtaaaga taatcttttc
    18661 catgcccccg ataaatgttg ttccggggaa aaagaaaatt tttcgttcaa caaaacaagt
    18721 tttttaacaa aaaatcaatt agttgaccaa aaacatccgt aaaaataaga gagagaaaaa
    18781 tttccctccc tcatttttca aattataaac tctcgccaaa atcatcctta aattttgcca
    18841 taagtttttg tggccaatcg cttaccggtt caagtcctcc cataaagaag cgcagaacag
    18901 gtggttcata aacaaatcga agtcctccga taagttgtcg attatcgtac aaccaattta
    18961 ctcggtcttc aacatattta atttgtgaaa gtgtaaaaac acggcgtgga acggctaaac
    19021 gcagcaattc catgtcggcg taagtctcat taccgtattc atcacgttgg ttcgaaatag
    19081 aaccgcgttc cattcctcga ataccggaaa tcagaaagag ggctgcagca agtgcacctg
    19141 caggatattc cttttgtgga atgtgagcgc atatctgcat ggcatccaaa tgaacaccaa
    19201 gcacacctgc cggttttatg acaggaatgc ctatggagtc gagtgcggcg acaagatatt
    19261 ttataaaatt tggactttgg cagataattg tttcatccaa tgtttcatat agtccaacgg
    19321 ccatcgcttc catttcgcgt accgacatac ctccataagt aagaaatcct tcaaaaagtg
    19381 gaaccagagc ttccaattcg tgataaattt cattactgtc ggtacaaatt ccaccacccc
    19441 gcgatgaact gagttttcgc gccgaaaaat aaaccaagtc agctaattcg gtcataattt
    19501 tcagaatttc acccaatgat ttatttttaa actcttcttc ccgttgttta atcaaaaaag
    19561 cattttctcc aatcaagctg gcatcgagta ccagacggat tttatattta tcggctattg
    19621 accttacatc acgtaaattt tgtatagaga atggttgtcc gccaatcaga tttgtagaag
    19681 cttccatacg aataaacggt atattttcag ctccgtgcag ttgaatgatt tcttccagct
    19741 tttcgagatt catatttcct ttgaaaggat gctcagaatc aattacatag gcttcatcat
    19801 agagtagttc ggcgattttt ccgccatatt gcgtgatgtg ggccatagct gttgtaaaat
    19861 ggtagttcat cggtattatg cttccttttt ttacataggc ataagaaata atattttctg
    19921 cagcacgacc ctgatgaaca ggcaaataat attttttacc caaaacatcc tgaacagctt
    19981 tttgaagtct gtaaaaactt tgtgatccgg cataagcatc atcggcctgc atcatgccag
    20041 ccatttgatt atcactcata gcattggtgc cgctatcggt caacatatcc agaaaaacgt
    20101 ctttagtact caatctgaat gtattgtatc caccttcctg caatgcttcc aatcgccttt
    20161 caatggggac aagatgtaat ttctgtacta ctctaacttt gtgtaattca aggggaatgt
    20221 tttcgccttt gtaaaatttg atttcttgca taataaaaaa ataaaaagtt aatactacat
    20281 tttgaagtta ttataatgat gtaaaatttt ttaattaaag tagttatcgg gatattattt
    20341 ctttcgttgg atgaaaatat ttttttctaa ataaaccatc gaatacaata ttcattacaa
    20401 aagtaattaa ttaattggta tttattgagt aaaattgtac ttattcttta gtagagtttt
    20461 gattaaatta tagtaatttt ttgtaaatat tgaagggcag ctttttcgaa ctgcccttca
    20521 ataaaaggaa aaatgacatt aaaaaaatcg aagctgatca acaagtcatg gttggcttaa
    20581 tcagtttgaa gaagtcgttt cccttgtcgt cgatcagaat aaaggcggga aaatcttcta
    20641 cctcgatttt ccaaacagct tccattccaa gttccgggaa atcgatacat tcaacttttt
    20701 ttatggaatt ttgagcgaga atggcagccg ggccaccaat agaacccaaa taaaaaccgc
    20761 catgtttttt acaggcatct accacttgct gactgcggtt gcctttggct atcatcacca
    20821 tactgccgcc atgttcctga aacaaatcta cataagtatc cattctaccg gctgtagtag
    20881 ggccaaacga accggaaggc attcctttgg gcgttttggc ggggccggca tagtacaccg
    20941 gatgatcttt catatattgt ggcaaatctt tccctgcatc gagcatttcc tttatgcggg
    21001 catgagcaat atctctggcc actaccattg ttcccgaaag agaaagtcgt gtggaaaccg
    21061 gatattttgt caatgtggac aaaatttcat tcatcggacg attcaaatcg attttcactg
    21121 cgtttccttc acctgtattg cgatattctt caggaatata tttaccggga ttatcctcca
    21181 gtttttccag ccagatacca tctttattta tctttgcctt aatgttgcga tcggcagaac
    21241 aagataccgc caatcctaca aagcaggaag ctccatgacg cgggagtcgg ataattcgca
    21301 catcgtgagc aaaatatttt cctccaaatt gagccccaat acccaaattt tgagctgctt
    21361 tcaggatttt ttcttccagt tccacgtccc tgaaagctct tcccaactca tttcctgtag
    21421 ttggcagttc gtcgtaatac ttggctgcag cgagtttaac cgttttcatg caagcatcgg
    21481 cagatgtgcc gccgataaca aatgcaatat gatagggtgg acaagccgac gtgcccaata
    21541 atttcaattt ttcggtaaga aatttttcca gcgtgtcggg ttgcagcaaa gctttcgttt
    21601 cctgaaacag aaatgatttg ttggccgaac cgcccccttt tgcgataaaa agaaattcgt
    21661 attcgttacc atgactggcc ataatatcaa tctgggcggg aagattggta ccggaattca
    21721 cttcgtcgta catggtaagt ggagcatttt gcgaataacg cagattttcc tgcgtataag
    21781 tttcataaac tcctttggat aaagcttctt catccgttcc atcagtgaaa acgttactgc
    21841 ctttcttggc ataaattgtt gccgtaccgg tatcctggca gaaaggaaga ataccttttg
    21901 cagaaatctc tgcattgcga agcatggtta atgctacgta tttatcgttt tcgctggctt
    21961 ccgggtcgga aaggatggcc gcaacctgtt tttgatgttc gggacgaagc agaaaagaac
    22021 agtcgcgaaa agcagtacgg gcaagaaaag tcaaaccttc cggctgaact tttaaaattt
    22081 ccttgccttc aaactctgcc gtcgaaacaa aatccttcga tagtaaataa tactcggttt
    22141 tgtcttttcc caactgaaaa agttcctgat atttgaattc ttttgccata aacgttataa
    22201 tttttttttg aatgagcgat agattgaaaa acgttgcaaa attacggaat tttttccgta
    22261 ataaaggaga aaaaattaga attttttcat tttcatagac tgcaccagaa agttaccaga
    22321 aaagggacag aaaagtccat gataaatccg tgataaatgg aaaccagcgc aaaatccttt
    22381 ccggcacttt gaacaatcac cgggaaagtg gtatccatag tagtagcgcc acctgaagaa
    22441 ataggagaaa gttttccaaa atattttacc atgagaggag cgaatagcaa agtgaaaatt
    22501 tcacgcacga tattcgacaa caaagcaata gtccccaact ctacgccttt gtagcgagtg
    22561 atcaggatac tggaaagcga ataataacca aatcccgaac ttgctgccag agagtcgaac
    22621 agagaaaagc cgtcaataaa gaaaccgact gccagcgtac ccaaccacgt accggcaatg
    22681 gtaaacagag gtaaaaagac aatgcgtggg ttcaagcgac gaaattggcg gacaaaattg
    22741 ccttgctgcc cgatactgaa tccaacggta aacatcaaac agcaaagcac ataaaagctc
    22801 atttcttcgt taaagagagt ttcgggaagc aacctgaaaa tgcctaaaag caagcctgtt
    22861 gcaaagaaag acaaaataat caggctactt ttcatgggtc gatttatccg gttttatcca
    22921 tttccacaaa aaagctgctc cgagtatact tcccaaagtt ccggccgttc caattaacag
    22981 tgcatcaaag ccgagctttc ccgtttgacg aataactgca ggattactgc caatttctgc
    23041 tcccaataag aaaagcaaca gccagattaa aacaaacaaa accgattgaa taattttgaa
    23101 atgttttttt cgaaaaataa agccgactac cataccggcc aacatacaac ctaatacagt
    23161 aaacatttgt aaatcatgcg aattatgaaa tcaatttaac aacttttatt caaaaacttt
    23221 tttcatcaat atatttgggg cttaattaaa actttggtaa attatcacaa aaagtgattt
    23281 ttgtattcta tttttaacaa acaactgaaa atcaacaaca ttgctgtaaa gagcagtttt
    23341 cttccgttgt ttcaaattcg atagtggcat gttcaatacc ttgttgaagc aattctgagc
    23401 gaattttatt ctttatttct acagcttttt ctattgtaac tggttgtttc aaaacgacat
    23461 gaacagtaag aatattataa tttccatcaa tagaccaaat atgcaaatcg tgaatttcat
    23521 ccacattttc aatcgacaga agcaattgtg aaattttttc caggtcgaca tccgttggcg
    23581 aaccctgaag aagaatgtgc aaactttcct ttacgttgcg aaaaacatga aacaaaatgt
    23641 aaattgtaat caaaacggat aaaacaggat caataaccgg caaatcaaag aaatacataa
    23701 tcagagaacc caccagaata gcagcccatc ccagcacatc ttccagcata tggagagaaa
    23761 caaccttctc gttgatagaa cttccttttt tgagacgaag aacagccaac ccgtttacta
    23821 caattcctat cacagcaaaa acaatcattc cttgcgtttt ggtcgtttcg ggatgtaaaa
    23881 gacgcggtat cgcttcgtaa aggacaaaaa tgctacccgt aatcaaaata atggaattga
    23941 taatggcacc caataaagaa aatcttttgt atccgtaggt gtaattttta tcactttctt
    24001 ttgtggattt tttttgaaaa taccatgcta aacccaatga aaggctatcg cccatatcgt
    24061 gtatggcatc ggaaataata gcaatactgt tggtgaaaat tccaccaaaa atttcaaaaa
    24121 cggtaaatcc gaaattaaga aaaaaagcca tttttatgtt gctctctcca tgtgtatgcg
    24181 tatgttgatg atgatgttcc attacttgta aattgaaatt tattaaaaac aattgagatt
    24241 ataaatggtt cgttttttgc attgaaaaaa gttcgacaaa atgatgattg aatctcctct
    24301 taaaacctga ttttttcaaa aaagagaaaa aataagattt ttttgaagag aggcattaaa
    24361 ttttttgttg tt
//
LOCUS       DAOSIB010000027        24367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_2297_length_24367_cov_13.452205, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000027 DAOSIB010000000
VERSION     DAOSIB010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 24367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24367
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2297_length_24367_cov_13.452205"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2213)
                     /locus_tag="PLD44_04025"
     CDS             complement(<1..2213)
                     /locus_tag="PLD44_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98534.1"
                     /translation="MDDKKEILTILEKIINDFSIDNLVRLFQNKTTKFRMLQSPASQP
                     LFTDGLLLGEFGCGDESVCIFTFKTHQPLTERSGKKAQYELAKKMLKEQIRYDGGFFI
                     FYDEQGNFRFSLIYDIPLSSGTREWSNFKRYTYYVGKEQTNKTFIQQMAEADFSSLNS
                     IIKAFSVEKVTREFYNDIANWYFWALQKVQFPKDAEQQSNGRNIALIRFITRIIFVWF
                     MKEKKLISPQLFDFDHIKNILKSTDDNESTYYKAILQNLFFATLNTPIPERKFRTENR
                     FQGKNEDYMNHEYFRYQELFSDPKQDINLLFNEIPFLNGGLFECLDKPKDDPSNDTGN
                     EIRMDGFSDKKENQPIFPNELFFSKEKNVDLTEYYGDTKYKNTRVQGLISILNAYNFT
                     VDENTPLDEEVALDPELLGKVFENLLASYNPETATTARKATGSYYTPREIVDYMVTES
                     LKYYFKTKFENTFPPEELDEKLTSLFSYSHEEQPFNEKETDFIIEKINELKIIDPAVG
                     SGAFPIGVLNKLVFLLSKLDPHNEKWKAQQFRALDYITDPTLKNELREKIENNFKRNE
                     LDYGRKLYLIQNCIYGVDIQPIAIHIAKLRFFISLLVDEKINPNIDDNFGIEPLPNLE
                     TKLVAANTLIGLPTSKQLVIKSDETKQIEAELFQIRKQHFTANYEKKKKEIQKRDREL
                     RNRLKELLEESGFPSDYTEKIASWDLYDTNRSAEWFDPEWMFGVKNGFDMVIGNPP"
     gene            complement(2295..2435)
                     /locus_tag="PLD44_04030"
     CDS             complement(2295..2435)
                     /locus_tag="PLD44_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98535.1"
                     /translation="MPNPIHGIIIINKINAAKQINKLQPSKNQLIWQRNDAEHIIRND
                     MN"
     gene            complement(2479..5892)
                     /locus_tag="PLD44_04035"
     CDS             complement(2479..5892)
                     /locus_tag="PLD44_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOF98536.1"
                     /translation="MNNFITNSNKKQLKTRLTELLSKSEELKFLVGFFYFSGLRELYD
                     GLQSNDKVKLKVLIGLNVDKTIYGLTEYGEKDKNLSDDERIENFFTSIRKSINSDVFD
                     NKDFYEQVKFFLQKIKDDEIILRKSREPNHAKLYIFKLEEGQIGRNKLFITGSSNLTS
                     AGLMSQAEFNVEISDYGFDEAESYFDELWNNAIKITENDELKEKIVTVVEHETLMKKI
                     TPFEAYLFVLKNYLDTFRGKEITQRLTEVLEENGYKAYRYQLDAVQQALSITENNNGV
                     IIADVVGLGKTIIACSIAFELKKRGIVIAPPGIIGDKAKSEGWKKYLEQFHLASLGWE
                     AFSLGDLEHVSEFVSKTNDIEVVIIDEAHRFRNEDTKDYEYLKNICRNKIVILLTATP
                     FNNKPADIFSLLKLFIVPKKSSITLDDDLENKFRTFKSEFDKLSYIKRYHQSSDRQKQ
                     NRALSYYNDLFGDQKIVDSTKIKKRTEILAKQIRDVIEPVIIRRNRLDIQNHPYYKNE
                     VNELSIVESPKEWFYELNKEQSVFYDKIIQQYFALPDDGGRFKGAIYKPFEYELQKVK
                     KGELGKEENFQYLQQFNLYDFMRRLLVKRFESSFGAFEQSVKNFINIHQIVLEFIQKT
                     DKYILDRNLLEKIYELDADEIENYLQEYSEKIKNNVYPKNHKVYELKKFERQTEFIQD
                     IQSDIALFQQILNELHKLNLVAKDPKAECLIEEVKTILDAPPENLHEPKRKVIIFSEY
                     TDTVNYLAPLLTQVFNARVLVVAGDLSASKIKEINKNFDASATQQADDYDILLTTDKI
                     SEGFNLNRAGMVINYDVPWNPVRVIQRVGRINRISRKVFDKLYIINFFPTEKGSDLVH
                     SREIAAQKMFMIHNTLGEDAKIFDIDEEPTPSGLYNKILQSPDAIEGESSYTKISKEF
                     EAWKEKYPDMVASLKNFPSRIKVAKDGAENELFVVIKKQKLFVHQQLYDAKEGSENHF
                     IKEVEDILEKIKADINEPPLPLSNQFWEEYEKIKNYQENPKNYKPIPDNNLQTRAINV
                     LRNFLKINDNQSVTILKPFVRMLLEDLLDYGTLSDYTIRRIANIDIDKNFTKAVDELK
                     SIEKELGSDYLYKEKQRISDVSKEIIIAIENKKTNELPTQNTFNDNISK"
     gene            6673..10152
                     /gene="ileS"
                     /locus_tag="PLD44_04040"
     CDS             6673..10152
                     /gene="ileS"
                     /locus_tag="PLD44_04040"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOF98537.1"
                     /translation="MKKFAEYSSLDLSEVNKKMLLRWKEKDIFHKSIQIREGEPTFVF
                     YEGPPSANGMPGIHHVMSRTIKDLFCRYKTMQGFQVKRKAGWDTHGLPVELSVEKSLG
                     ITKEDIGKKISIEEYNAACRRDVMKYTREWEDLTTKMGYWIDLDDPYITYDNRYIETL
                     WWLLKQLYDKKLLYKGYTIQPYSPAAGTGLSTHELNQPGCYRDVKDTTCVAQFKVKDT
                     SSLPFKTKINGDCYFLAWTTTPWTLPSNTALCVGPNITYVLVKTFNPYTGKPIKVILA
                     KDLVNTYFNQKNENLPFENYQPGDKNIPYRILDEFKGSELVGIRYEQIIPWVNPGENA
                     FRVIAGDFVSTEEGTGIVHIAPTFGADDDRVAKQNNIPPMLLLDKDGNKQPMVDRTGK
                     FFKLEDLDPEFVSKNVNISLYREYAGRYVKNEYISAFEPENQSDKNSSGEKTEDSTLD
                     IDLCVMLKQRGLVFKIEKYTHSYPHCWRTDKPVLYYPLDSWFIKTTALRDEMIALNQT
                     INWKPASTGYGRFGKWLENLQDWNLSRSRFWGTPLPIWHTEDGSEEICIGSVDELISE
                     IEKSIQAGFMTSNPYKNFKSGDYSRENYAVQNIDLHRPYVDNIVLVSKSGKPMRREPD
                     LIDVWFDSGSMPFAQLHYPFENREAIDQHTFYPADFISEGVDQTRGWFFTLHAISTMV
                     KGSVAYKNVVSNGLVLDKNGNKMSKRLGNVVDPFETIEKYGSDPLRWYMMTNSAPWDN
                     LKFDIEGVEEVRRKFFGTLYNTYSFFALYANVDEFTCSEPEIPVENRPEIDRWIISLL
                     NTLIKEVGEYYEDYDPTRAGRAISDFVTENLSNWYVRLNRKRFWGGEYNKDKISAYQT
                     LYTCLETVAKLMAPIAPFYADQLFLDLNEVTEKNNCESVHLSNFPVYDEKLIDKNLEE
                     CMQLAQQISSMILSLRRKAGKKVRQPLSKAIVPAADDKIYQQISYVADLIKAEVNIKE
                     LQVIPANMEMANLTKKVKLNFKVLGKKYGKQMKEIAEATAKLSNKEIAELEQNGIYIL
                     NLPGGEVQLTTDDVEIITEDMPGWLTANEGKLTVALDITVTDELLKEGIARELVNRIQ
                     NIRKQNGYEITDRITVEIEKRDEINDAVTEFSNYISSQTLAAGISLVDKLEDGIELDF
                     DEYMVKVKVEKTR"
     gene            10288..10665
                     /locus_tag="PLD44_04045"
     CDS             10288..10665
                     /locus_tag="PLD44_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOF98538.1"
                     /translation="MSEKTRYSDEELEEFRQIILEKLEKAQKDYELLRNGVTNSDGND
                     VTDTSPTFKVLEEGASTLSKEEAGRLAQRQLQFIQHLQAALVRIENKTYGICRETGKL
                     IPKERLRAVPHATLSIEAKKSKK"
     gene            10749..11357
                     /locus_tag="PLD44_04050"
     CDS             10749..11357
                     /locus_tag="PLD44_04050"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOF98539.1"
                     /translation="MSTTKKSVLLIIFVLLVDQASKFYIKTHFALGDHIDIFSWFQIY
                     FIENNGMAFGIELLDKLFLTAFRIGAVVLIIFYIRSLIKRNFRTGYVLSISLLLAGAA
                     GNIFDSVFYGVLFSDSYGRVATFLPPEGGYAPLFYGKVVDMLYFPLIKNAAGETIFFR
                     PVFNVADAAITISVFIILIFYRKELNASFEKKEKTNVAEQSK"
     gene            11335..12192
                     /locus_tag="PLD44_04055"
     CDS             11335..12192
                     /locus_tag="PLD44_04055"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HOF98540.1"
                     /translation="MLLSSPNKIFSFFLLLALFLLPACDNRPKNVLSKKEMVKMLTEY
                     HLLHGTLQTTNQYYTPEGTLYFESLLKKHGITKAEFDSSMVWYAKNPKKFERIYAEVL
                     QNLNHLREDIVKGKFRSPDSILPGIEISNLWNLHPSYRFTPDSARTKLPFKIQQPYLM
                     TQDVYELQFLERIAPEDSCVDPYVVMKIHYANGKTDSIFQKTFNDSILRRYTLRLRAR
                     FPYKVDSVTGLLLGCKQYNGNFNVQLDSIRLLRYFVPQWQDSLKQVVEKNAAPPQKIQ
                     TKTIRPKEN"
     gene            complement(12366..12887)
                     /gene="ribH"
                     /locus_tag="PLD44_04060"
     CDS             complement(12366..12887)
                     /gene="ribH"
                     /locus_tag="PLD44_04060"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOF98541.1"
                     /translation="MSTEYKNLSSYNEDEMPDKSKVFLQQYGIVVADWNPQITHAMLE
                     GAYNALIENGSNPELINIVHVSGTFELTYGAKQLLEQYEEMDHIKRHKYAAIIALGCV
                     IQGETPHFNYVCEGVTNGIAYLNANAVGCPVIFGVLTTKTLQQALDRAGGINGNKGVE
                     AAITAIKMANIDW"
     gene            complement(12966..13643)
                     /locus_tag="PLD44_04065"
     CDS             complement(12966..13643)
                     /locus_tag="PLD44_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOF98542.1"
                     /translation="MSKKKGKGQDELANVEHALSASELFFEKYQKQILYVVGALIMVV
                     LVVLAVRNFYLTPREKAAQNEMYHAQYYFNLDSFQVALNGKEPEFMGFKQIVSEYGIT
                     PSGNLAAAYAGICYYKLGQYENAIKYLTQFDGNNSYFSTSVIGLLGDCFVEIGQNDKA
                     LTYFNKAIAVENEVLSPLYLKKAGLLCESMNQLKKAEEYFLQIKEKYPKSMEAGDIDK
                     YLARVQN"
     gene            complement(14121..16094)
                     /locus_tag="PLD44_04070"
     CDS             complement(14121..16094)
                     /locus_tag="PLD44_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOF98543.1"
                     /translation="MKNRYRSLFFAFFLALLPLSFTHAVKAYPFPVQITQPDGTVLTI
                     RIHGDEFHHIKTTVDGYLVKQNAKGYWTYASQDAAGRITPSEVIARNVSSRSAQDIQF
                     LKSLKISSSVSLQHVKASGRQFQKPQLSARKNAFPLSNSPRSLVILVNFSDKSFVTSS
                     AQSAFTNLLNQSGYSANGATGSARDYFMASSYGKFSPEFDVVGPYTLSKTMQSYGAND
                     ADGYDVDPARMIVDACKAADNAGVNFSQYDTDNDGLIDNIFVYYAGYNEAEGASENTI
                     WPHRWVVYTSQESSTDYTYDGTVASVTFDGVRLYDYACTSELKGTSGSNMCGVGTFCH
                     EFGHVLGLPDYYHTAEDKTTLDYWNIMDAGNYLNDGRTPPVYSTYDRFYLGWLTPEEA
                     NTPSNLTLKPIYQGTTQPANTHQQSYLLSATTHNLVGNNPSPKEFFVLEYRKKTGWDA
                     YLPAEGMLIWHIDYDQTAWDNNEPNNYTGTTQTASSHMRVYLQPLSGSTTTPGTAFTS
                     GSFTPTTWSGTNINRAITEISKSSDQVTFKLMGGEIEDPNKAVIKLGVIQSQLVFPYT
                     AVNNAALKVINIKTNGITGNLTVTLSGDQAEMFSVSANSITQNAANSTDGVNLNITYR
                     PTTSGEHTAVLTISGGGLSPDKVILLKGTGQPQ"
     gene            16890..18335
                     /locus_tag="PLD44_04075"
     CDS             16890..18335
                     /locus_tag="PLD44_04075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF98544.1"
                     /translation="MLNIQKKMSNAFFALLSLPSTAMGFALSVQISALSWILSTKYGL
                     AIHEIGIVWAAGPIAGIIAQPIVGAISDKLWFMGGRRRPWIVLGGTLAALMLLALPNL
                     EVIQKIIVGGSDPQAGLLTIAIVVALTLDLSINVSFNPTRSLVADCTPLSERSKGFTW
                     MQTISGSFGVLAYLIGGTLGNIFLIYFGVGVAFLASVIPAFLIEEPRELKNFDAETQS
                     PKSKTTFRELWEAMLPLSGFLLYGIYVIFSKLGGFELKPTIPLFGYYLNVIEFLSILL
                     LLVSAIAVLLNLFKSGKKSNEFQKVLLAHSFTWIGVQTMFIYALFYLKDVLQLADNAG
                     SVNSIAFFVLSLVSAVLPVLVLNRLAEKYGMVKTHLSCIFIMALGYASILFFGHSVAV
                     YYFLIAIVGIGWGSVVSLPFAIMSEKVDQTKMGLYMGIFNLAVVLPQLVASFKMGEVI
                     NAAPNKSVIFAICAVTLFISGILWLFVKNEK"
     gene            18505..19146
                     /locus_tag="PLD44_04080"
     CDS             18505..19146
                     /locus_tag="PLD44_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98545.1"
                     /translation="MFLNIPLLEWIGYLASIIVAISLTMSSIKKLRWLNLIGSAVFSF
                     YGFSIGALPVGFLNLFIVLVNIYYLIRMNSAQESFKALAVEPNDAYFNYFIEFHKKEI
                     LQIFPHFELASTKQDKTSSIALLLLRNAVAAGVFYGTIDNGCLYVELDFVASEYRDLK
                     PGEFIYKTNVQQLKETGIHKLVAKSEHPQHHKYLIKMGFVRRNDTPAVFEKEI"
     gene            complement(19323..21461)
                     /gene="pnp"
                     /locus_tag="PLD44_04085"
     CDS             complement(19323..21461)
                     /gene="pnp"
                     /locus_tag="PLD44_04085"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOF98546.1"
                     /translation="MTVVTKTITMSDGREIVIETGKLAKQADGSVMVRMGDTMLLATV
                     CSAQDANEGTDFMPLSVDYKEKFAAFGRFPGGFLRREGRPSDYEILISRLVDRALRPL
                     FPDDYHAETFVNIMLCSADGENIPDALAGLAASAALAVSDIPFNGPVSEVRVARIDGN
                     FVVNPTFNQLENADMDIIVAATMDNIMMVEGEMKEVSEDDLLDAMKVAHEAIKLHCKA
                     QLELMETVGKTKKREYCHEQNDEELKKDIWEKTYDKVYAEAASGNADKHSRSEHFEAI
                     MNEYIASLDTETAEAKKPLIERYYHAIEKEAMRRAILDEGKRLDGRKTTEIRPIWSEV
                     DYLPGTHGSSIFTRGETQALATVTLGTKMDEKLIDEPLIQGKERFLLHYNFPPFATGE
                     ARASRGIGRREIGHGNLAMRALKNVIPDDYPYVIRVVSDILESNGSSSMATVCAGTLA
                     LMDAGVQIKKPVAGIAMGLISENQGKNYAVLSDILGDEDHLGDMDFKVTGTREGITAT
                     QMDIKVDGLSYEILEKALRQAKEGRLHILNKMLETISEPRADFKPHVPRIVVMFVPKE
                     MIGAIIGPGGKIIQNIQLETGATITIEEIGDQGRVEIAATNKDSIEAAINKIKGIVAI
                     PEVGETYIGKVKSIMSYGAFVEFLPGKEGLLHISEIEWKRTESMDQTGIKEGDMIEVK
                     LLDIDEKTGKFKLSRKALLPRPPKEEKPRN"
     gene            complement(21718..22530)
                     /locus_tag="PLD44_04090"
     CDS             complement(21718..22530)
                     /locus_tag="PLD44_04090"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOF98547.1"
                     /translation="MLEKIQETADFLLAKMPMRPKTGIILGTGLGNLVTQMDIEMEIP
                     YETIPNFPVSTVEGHKGKLILGKLGGVEVLAMQGRFHFYEGYDMKAVTFPVRVMKALG
                     IKNLLVSNAAGGMHPDFEIGDLMIITDHINLFPEHPLRGKNFEELGPRFPDMSEAYCK
                     DFIVKAKQIAERNHIKVVEGVYVGTSGPTFETPAEYRYFRAIGGDAVGMSTVPEVIVA
                     NHAGMKCFGISIITDLGVPGKIVEVSHEEVQKIGDSVQPLMTLIMKELVKEI"
     gene            complement(22502..23590)
                     /gene="lpxK"
                     /locus_tag="PLD44_04095"
     CDS             complement(22502..23590)
                     /gene="lpxK"
                     /locus_tag="PLD44_04095"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOF98548.1"
                     /translation="MNRELKHIALYPFSLIYGWVADIRNWMFDEKIFHSVGFDVPTIV
                     LGNLAVGGTGKTPHAEFVLSHLQHEFKTAFLSRGYKRKTKGFVLADKNASAATIGDEP
                     YQIFRKFPNVKVAVDEKRVEGIKRLLKLFPDLQLIVLDDAFQHRYLLPGFSVLLTDYS
                     NLYTSDFFLPAGSLRESKEGSHRANLIVVTKCPENLKPIDKRIIEMELHPLPYQQVFF
                     STFRYHDPVPVFPELTTENWSLSKLREESPAVLMVTGIASPQSMKDFLKDYVSTLKEV
                     SFPDHHKFSKNDFQLIRQEWDALPSENKIIIVTEKDAARLYNEKYLPETLKAKIFALP
                     IEVKFLFDQESLFIQKLKDYVRENSRNR"
     gene            complement(23755..23976)
                     /gene="yidD"
                     /locus_tag="PLD44_04100"
     CDS             complement(23755..23976)
                     /gene="yidD"
                     /locus_tag="PLD44_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOF98549.1"
                     /translation="MKKFFRWLVLLPVYFYRYSISPLFPPSCRYTPTCSQYTIEAIKK
                     YGVFKGGWLSVKRISRCHPWGGSGYDPVP"
     gene            complement(23973..>24367)
                     /gene="rnpA"
                     /locus_tag="PLD44_04105"
     CDS             complement(23973..>24367)
                     /gene="rnpA"
                     /locus_tag="PLD44_04105"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOF98550.1"
                     /translation="MERTRFLFPKSERLNSKIKIDRLFTEGKAFLVYPLRTVYYVSAE
                     NPAGVEVLISVPKKKFKRAVHRNRIKRLIREAYRLNKNELSDVVLSEQRQMQVAFLYV
                     ADKEINFHELNDKMKQILEQLQKIASEQ"
BASE COUNT         7150 a         4576 c         4748 g         7893 t
ORIGIN      
        1 ggcggattgc caatgaccat gtcgaatccg ttttttaccc caaacatcca ttcgggatcg
       61 aaccactcgg ccgaccggtt ggtgtcgtat agatcccacg aggctatttt ttcggtataa
      121 tcggaaggga aaccactttc ttcgagcaat tctttcagcc gatttctcaa ttcgcggtct
      181 cttttttgaa tttctttctt tttcttttcg taattggctg taaaatgttg cttgcgaatc
      241 tgaaaaagtt ctgcttcaat ttgttttgtt tcatcacttt taataaccag ttgtttagat
      301 gtgggcaatc caatgagcgt attggcagcc actaattttg tttcaagatt gggcaaaggt
      361 tcgatgccga aattgtcgtc aatattcggg tttatttttt cgtccaccag caaagaaata
      421 aaaaatcgaa gtttggctat gtgaatggca ataggttgaa tatccactcc gtaaatacaa
      481 ttttgtatca gatagagttt tcgtccgtag tcgagttcat ttcgtttgaa attgttttca
      541 attttttcgc gaagttcatt ttttaatgtg gggtcggtaa tgtagtcgag tgcccggaat
      601 tgttgggctt tccatttttc gttatgcgga tcgagttttg aaagcagaaa aacgagctta
      661 tttaaaacgc caatggggaa agcacctgaa ccgacagcag ggtcaatgat tttgagctca
      721 ttgatttttt caataatgaa atcggtttct ttttcgttga aaggttgctc ttcgtggctg
      781 tatgaaaaaa gtgaagttag tttctcgtca agctcttcgg gagggaaagt gttttcaaac
      841 ttggttttaa aataatactt gagcgattcg gttaccatat aatccacaat ttcgcgtggc
      901 gtataataac ttccggtagc tttgcgtgct gtggttgctg tttcgggatt gtaggaagcc
      961 agcaggtttt cgaatacttt tcccaacaac tcaggatcga gcgccacttc ttcgtcgagg
     1021 ggagtgtttt catctaccgt gaaattgtag gcatttaaaa tagaaattaa cccttgtact
     1081 ctggtatttt tatattttgt atcaccataa tattccgtca ggtccacgtt tttttctttt
     1141 gaaaagaaaa gttcgttggg aaagatgggt tgattttctt ttttatccga aaatccatcc
     1201 atgcgaattt cgttgcccgt atcattggaa ggatcgtctt ttggcttatc cagacattca
     1261 aacagtccgc cgttgagaaa aggaatttcg ttgaacagaa ggttgatatc ctgtttggga
     1321 tcagagaaaa gttcttgata acgaaaatat tcatgattca tgtaatcttc gtttttaccc
     1381 tgaaatctat tttctgttct aaattttctt tcgggaatgg gcgtattaag tgtcgcaaaa
     1441 aataaatttt gaagaatggc tttgtagtaa gtactttcgt tgtcgtctgt gcttttcagg
     1501 atatttttaa tgtggtcaaa atcgaaaagc tgaggactga tcaatttttt ttccttcata
     1561 aaccatacaa aaatgatgcg ggtaataaaa cgaataagag caatgtttct tccattggat
     1621 tgctgctccg catcctttgg aaattgcacc ttttgcagtg cccagaaata ccagttggca
     1681 atatcgttgt agaattcacg ggtaactttt tctacgctga atgccttgat gatggagttg
     1741 agcgaagaaa aatcggcttc ggccatttgc tggataaagg ttttgttggt ttgttctttg
     1801 ccaacgtagt aggtgtagcg tttaaaattg ctccattctc ttgttccgga cgataaagga
     1861 atgtcataaa tcaaagagaa acgaaaattt ccctgttcgt cgtaaaaaat gaaaaatccg
     1921 ccatcataac gtatttgctc tttcagcatt ttctttgcca attcgtattg tgcctttttg
     1981 cccgagcgtt cggttaaagg ttgatgggtt ttgaaggtaa aaatacaaac actttcatcg
     2041 ccgcaaccaa attctcccaa caataaaccg tcagtaaata atggttggga tgcaggcgat
     2101 tgcagcatgc gaaatttggt ggttttgttt tggaacaaac gaacaaggtt atcgattgaa
     2161 aaatcgttga tgattttttc taaaatggtc agaatttctt ttttatcatc catacgtttt
     2221 ttggtttatt gttatgaaat ttttatcgtt ttcccatttc aaaggattct caaaaatata
     2281 ttggcgaatg cgatttaatt catgtcgttg cgaataatgt gttcggcatc attccgttgc
     2341 caaatcaatt gattttttga tggttgcaat ttattgattt gttttgcggc attgatttta
     2401 ttgataatga tgatgccgtg aatagggttt ggcattataa tccatttatt gaattgaacg
     2461 aaggattcca ttcaatattt attttgaaat gttgtcgttg aatgtatttt gtgttggcaa
     2521 ttcattcgtt tttttgtttt cgatggcaat aataatttct ttcgagacat cggaaatacg
     2581 ttgtttttct ttgtataaat aatcgctccc caattctttt tcaatggatt tcagttcatc
     2641 aactgctttt gtgaaatttt tatcaatgtc aatattggca attcttctga tggtgtaatc
     2701 ggaaagcgta ccgtaatcga gcaaatcttc cagcagcatt cttacaaaag gtttcagtat
     2761 ggtaaccgat tgattatcat ttatttttaa gaaattacgc aacacattaa tagcacgcgt
     2821 ttgcaggtta ttatcaggaa taggtttata gttttttgga ttttcctgat aatttttaat
     2881 cttttcgtat tcttcccaga attggttact caagggtaaa ggaggttcgt ttatgtcggc
     2941 ttttattttt tcgagaatat cttccacttc tttaataaaa tgattttcac ttccttcctt
     3001 tgcatcatag agctgttgat gaacaaaaag cttttgtttt ttgataacca caaacaattc
     3061 attttctgca ccatctttgg caacttttat gcgcgaagga aaatttttca gcgaagccac
     3121 catgtcagga tatttttctt tccatgcttc aaattctttc gatattttcg tataggaact
     3181 ttcgccttcg attgcatctg gactttgcag aattttgtta taaagtcctg atggcgtagg
     3241 ttcttcgtcg atatcgaaaa ttttggcatc ttcgcccagc gtattgtgaa tcatgaacat
     3301 tttttgtgcc gcaatttctc ttgaatgaac cagatccgaa cctttttcgg tagggaaaaa
     3361 gttgataatg taaagtttat caaaaacttt tctgctgatg cggttgattc ttcctacacg
     3421 ctgaatcacg cgtaccggat tccagggaac atcgtaattg attaccatgc cggcacgatt
     3481 caaattgaat ccttccgaaa ttttatcggt agtcaataag atatcataat cgtcagcctg
     3541 ttgagtagcg gaggcatcga aatttttatt gatttctttg attttacttg ccgacaaatc
     3601 tccggcaacc accagtacac gtgcgttgaa aacttgggtg agcaagggag ccagataatt
     3661 gaccgtatcg gtatattccg aaaaaatgat aacttttctt ttcggttcat ggaggttttc
     3721 gggcggagcg tcgagtattg tttttacttc ttcgatgagg cattcggctt tggggtcttt
     3781 tgctaccaga ttcagtttgt gcaattcgtt aagaatttgc tggaataatg caatatctga
     3841 ttgaatatcc tgaataaatt cggtttgacg ttcaaatttt ttcagttcat acactttatg
     3901 gtttttagga tatacattat ttttgatttt ttcagaatat tcttgcaaat aattttcaat
     3961 ttcgtcggca tccaattcat aaattttttc cagcaaattc ctgtcgagaa tatatttatc
     4021 ggttttttgg ataaattcca gtacaatttg atggatgttg atgaagtttt tcacgctttg
     4081 ttcaaaagcg ccaaatgaac tttcgaaacg ttttaccagc aatcgccgca taaaatcgta
     4141 caagttgaat tgttgcaaat attggaaatt ttcttctttg cccagctcac ctttttttac
     4201 tttttgcaac tcatattcaa aaggtttgta aatggctcct ttaaaacgtc ctccgtcatc
     4261 aggcaaggca aagtattgtt gaatgatttt gtcgtaaaat accgattgtt ccttgttcag
     4321 ttcatagaac cattcttttg gagattctac aattgagagc tcatttactt catttttgta
     4381 gtagggatga ttttgaatgt ccagccgatt tcggcgaata atcaccggct caataacatc
     4441 gcgaatttgt tttgccagaa tttctgttcg tttttttatt ttagtcgaat cgactatctt
     4501 ttggtcgcca aataaatcat tataataaga aagggctctg ttttgctttt gccggtcaga
     4561 cgattggtga tatcgcttga tgtaggaaag tttatcaaat tccgatttga atgttctaaa
     4621 tttgttttcc aaatcatcat caagggtaat acttgatttt ttgggaacaa taaagagttt
     4681 gagcagcgaa aatatatcgg ctggtttgtt gttgaaagga gtagcagtga gcaagataac
     4741 gattttgttg cgacaaatat ttttcaggta ctcgtaatct ttggtatctt catttctgaa
     4801 tcgatgcgct tcgtcaataa ttaccacttc gatatcgttg gttttcgaaa caaattccga
     4861 aacgtgttcg aggtcgccaa gtgaaaaagc ttcccagcca agagaagcca gatgaaattg
     4921 ttcaagatat tttttccagc cttccgattt ggctttatcg cctatgatgc ccggaggtgc
     4981 aattacaatt ccacgctttt tgagttcaaa agcaatactg caagcaataa tggttttgcc
     5041 caatcccact acatcggcaa tgattacgcc gttgttgttt tcagtaatgg aaagtgcttg
     5101 ttgaacggca tctaactggt aacgataggc tttgtatcca ttttcttcaa gcacttcggt
     5161 aagccgctgc gtaatttctt ttccacgaaa ggtgtcaaga taatttttca aaacaaaaag
     5221 ataagcttca aaaggcgtaa tttttttcat cagcgtttca tgttcaacaa ctgtgacaat
     5281 tttttccttc aactcgtcgt tttctgtaat ttttatggca ttattccaga gttcatcaaa
     5341 ataactttct gcttcatcaa aaccataatc gctaatttcg acattaaatt ctgcttgcga
     5401 cattaatccg gctgaagtca gattgctgct gcccgtaata aatagtttgt tgcgcccaat
     5461 ttgtccttct tccagtttaa aaatgtagag cttggcatga ttgggttcgc gtgattttct
     5521 gagaataatt tcgtcatcct ttattttttg aaggaaaaat ttcacctgct cataaaaatc
     5581 tttattgtca aaaacatctg aatttatgga ctttcggatg gaagtaaaaa agttttctat
     5641 tcgttcgtca tcagaaaggt ttttgtcttt ttcgccatat tcggtcagtc cgtaaatggt
     5701 tttgtccaca tttaagccga tcagcacttt tagtttcact ttatcgttcg attgcaagcc
     5761 atcgtacaat tctcttaaac cagaaaaata aaaaaatccg accagaaatt tcagctcttc
     5821 acttttggat agaagttcgg tcagacgtgt ttttaactgc tttttgttgc tgttggtaat
     5881 gaaattgttc ataatttttt attgatcaat aaaaaaatta aactgaaaag gaaaattctt
     5941 tttaaaatga ttcggcaaaa tatcttttaa taaattggca gcttattgat taaaaagcaa
     6001 agtttcacgc aaaaaaattg attaagcagc tgatggtcag tggatgcatg ctaatcccaa
     6061 acactttaaa gatgcaacaa accgcaaact caatcaatat aggagtttct gtagtaacta
     6121 ttagttatcc cggctacgaa gttaaataaa agttttaaat cttaccttaa tggagatatt
     6181 aattttatct gccgtgatgt cttgggtcgc tgtatcttaa tattttggtc gggtacgggc
     6241 aaaaattaag ggatttcttt ttcaatataa ttttgtaaaa caatgatata caaagcctgc
     6301 tggtaagcct tcggacattt ttccgatata tttttaacaa actgttgatg ttgaaaaacc
     6361 gaatcggaat cgggcgatgt agttttcgga gaaataaatc aaggaggaaa caccaaacaa
     6421 ggcaaccttt ttttcttcaa ataattatta ctctttcagt tccataacca atgcccgaat
     6481 acttctttgt attcgacttt tcgtttttca ataaaattta gtaattttgc accttccaaa
     6541 aaaacagcaa agaaagccga aaaaagcagg aaggaatctc agaaattgag ttctatcaaa
     6601 aatttttatg caattcgggt tttcagcgtt tttgctattg gttaaagaca ggaatcagag
     6661 gataaacacg atatgaaaaa gtttgcagaa tactcttctt tagatctttc ggaagtcaac
     6721 aaaaaaatgc ttttacgttg gaaagaaaaa gacattttcc acaaaagcat tcaaattcga
     6781 gaaggagaac caacgtttgt attttatgaa ggcccgcctt cggccaacgg tatgccgggc
     6841 atccatcatg ttatgtcccg aaccataaaa gaccttttct gccgttataa aaccatgcag
     6901 ggttttcagg ttaaaagaaa agcaggatgg gatacgcatg gacttccggt tgagttgagc
     6961 gtggaaaaat cattgggaat cactaaagaa gatattggta agaaaatcag tatagaagaa
     7021 tacaatgcag cttgtcgtcg ggatgtaatg aaatataccc gcgagtggga agatttgacc
     7081 acaaaaatgg gttattggat tgatttggat gatccttaca ttacctacga caatcgttat
     7141 attgaaacac tttggtggtt gctcaaacag ctttatgata aaaaattgct ttataagggc
     7201 tataccatac aaccctattc accggcagca ggaaccggat tgagcacgca cgaactgaat
     7261 cagccgggtt gctatcgcga tgtaaaagat actacctgcg ttgctcagtt caaagtgaag
     7321 gatacttctt ctttgccatt caaaactaaa ataaatggcg attgctattt tctggcatgg
     7381 accactacac cttggacttt accttccaat acagccttgt gcgtagggcc gaatattacg
     7441 tatgtgcttg taaaaacgtt caatccttat accggcaaac ccataaaagt aattttggcc
     7501 aaagatttgg taaacactta tttcaaccaa aaaaacgaaa atcttccttt tgaaaattat
     7561 cagccgggtg ataaaaatat accttaccgc attttggacg aattcaaagg cagcgaattg
     7621 gtcggcattc gatacgaaca gataattccg tgggttaatc ccggagaaaa tgcttttcgt
     7681 gttattgcgg gagattttgt ttctacggaa gaaggaaccg gaatcgttca cattgcacca
     7741 acttttgggg ctgatgacga tagggtggca aaacaaaata acattccgcc gatgctgttg
     7801 cttgacaagg acgggaacaa gcaaccaatg gtggacagaa cgggtaaatt ttttaaattg
     7861 gaagaccttg atcccgaatt tgtttctaaa aatgtaaata tttcgcttta cagagaatac
     7921 gccggtagat atgtaaagaa tgaatatatt tcagcttttg aaccggaaaa tcagtcagat
     7981 aaaaattcgt ctggagaaaa aaccgaagat tcaacgctgg atattgactt gtgcgtcatg
     8041 ctcaaacaac ggggattggt attcaaaatt gagaaataca cacacagtta tccgcattgc
     8101 tggcgaaccg acaaaccggt gttatattat ccgctggaca gctggtttat taagactacc
     8161 gctttacggg atgaaatgat tgcattgaac caaaccatca attggaaacc tgcttctaca
     8221 ggatatggac gttttggaaa atggctcgaa aatcttcagg attggaatct ttcacgaagt
     8281 agattttggg gaactccgct tcccatttgg cacactgaag atggttcgga agaaatttgc
     8341 attgggtcag tggatgaatt gatttcagaa attgagaaat ccatacaagc cggttttatg
     8401 acatccaatc cctataaaaa tttcaaatcc ggcgattatt cacgtgaaaa ttatgctgtt
     8461 caaaatatcg atttgcatcg tccttatgtg gacaatattg tgctcgtatc taaatcggga
     8521 aaacccatga gaagagagcc ggacttgatt gacgtttggt tcgattccgg ttcgatgcca
     8581 tttgcacagt tgcattatcc tttcgagaac agagaagcca ttgatcaaca tactttttat
     8641 cctgccgatt ttatttcgga aggggtagat cagacacgtg ggtggttttt tactttacat
     8701 gccatcagca caatggtgaa aggttcggta gcctataaaa atgttgtttc caatggcttg
     8761 gtgctcgaca aaaatgggaa taaaatgtcg aaacgtttgg gcaacgtggt agatcctttt
     8821 gaaaccatcg aaaaatacgg ttctgacccc ttgcggtggt atatgatgac caattctgct
     8881 ccctgggaca atctgaaatt tgatattgaa ggagtggaag aagtacgaag gaaattcttt
     8941 ggaacgcttt ataatactta ttctttcttt gctctttatg ccaatgtaga tgaatttact
     9001 tgcagcgagc ccgaaattcc tgtggaaaat cgtccggaaa tcgaccgatg gataatatca
     9061 ttgttaaata cattgataaa ggaagttggt gaatattacg aagattacga tccaacaaga
     9121 gcaggcagag caatttccga ttttgttacc gaaaatctga gcaattggta tgttcgcttg
     9181 aatcggaaaa gattttgggg tggagaatac aacaaggata aaatctcggc ttatcaaacg
     9241 ctttatacct gtttggaaac cgttgccaaa ttaatggctc caatcgctcc gttttatgcc
     9301 gaccaattgt ttctcgactt gaatgaagtt acggaaaaaa ataattgcga atctgttcat
     9361 ttgagcaatt ttcccgttta cgacgaaaag ttaatcgaca aaaatctgga agaatgtatg
     9421 caactggctc agcaaatctc ctccatgatt ctttctctgc gtcgaaaagc cgggaagaaa
     9481 gttcgtcagc cactttctaa agctattgtt ccggctgccg atgataaaat ctatcagcaa
     9541 attagttatg tggccgattt aattaaagcc gaagtaaaca taaaagaatt gcaagtgata
     9601 ccggccaata tggaaatggc aaatttaacc aaaaaggtaa aactcaattt taaggttttg
     9661 ggtaaaaaat acggcaaaca aatgaaagaa attgcagagg caaccgcaaa attgagcaac
     9721 aaagaaattg ccgaactgga gcaaaatgga atttatattt taaatttgcc cggtggggaa
     9781 gttcaactta caacagatga cgtcgaaata ataacagagg atatgccggg ctggcttact
     9841 gccaatgaag ggaaacttac cgttgcgctg gatattaccg tgacggatga attgctcaaa
     9901 gaaggaattg cgcgcgaatt ggtaaacaga attcaaaata tacgcaagca aaatggctat
     9961 gaaataaccg acagaataac tgttgaaatt gaaaaaagag atgaaatcaa cgatgccgtt
    10021 acggaatttt cgaactatat ttcctctcaa acattggctg ccggcatttc tttggtagat
    10081 aaactggaag atggcattga gcttgatttt gatgaatata tggtgaaggt taaagttgaa
    10141 aaaacacgct gaaaaagcca caaaattatt actaaaataa ttggaaaaga gaaaaaaaac
    10201 gattattttc gcacattatt aaaaactcag catttgcaga atgctgaacc gaaacaaagg
    10261 gattaaacaa aataaagttt tacgattatg tcagaaaaaa caagatattc ggatgaagag
    10321 ctggaagaat ttcgtcagat tatcctcgaa aagttggaaa aagctcaaaa agattacgaa
    10381 ttattacgca acggtgttac caatagtgat gggaatgatg taaccgatac ttctccaacc
    10441 tttaaggttt tggaagaagg tgcatctaca ctttcaaaag aagaagccgg acgtttagct
    10501 cagcgtcagc tccaattcat tcagcatttg caggctgctc ttgttagaat tgagaacaaa
    10561 acatacggaa tttgtcgcga gaccggcaag ctgatcccta aagaacgctt gcgtgccgtt
    10621 cctcatgcta cactaagtat tgaagccaaa aagtcgaaaa aataatttat atattctatc
    10681 cactattaaa gacggcaaca gagtattttt ctttgttgcc aattttttaa taaaagcagc
    10741 ttttgttcat gtcaactaca aaaaaatcgg ttttattgat aatctttgtt ttgcttgtag
    10801 atcaagccag caaattttac atcaaaacgc attttgcttt aggcgatcat atcgatattt
    10861 ttagttggtt tcagatttat tttatcgaaa acaacggaat ggctttcggc attgaattgt
    10921 tggacaagct tttcctgact gctttcagaa ttggcgcagt tgttttgatc atattctaca
    10981 ttcgatcgct aattaagcga aattttcgta ccggttatgt gttgtccatt tcattgcttt
    11041 tggccggcgc agcaggaaat attttcgact ctgttttcta tggcgtcctt ttcagcgaca
    11101 gctatggaag agtggctact tttttacctc ccgaaggcgg ttatgccccg ctattttacg
    11161 gaaaagtggt cgacatgctt tatttcccgc ttataaaaaa tgctgcaggt gagacaatat
    11221 ttttccgacc tgttttcaat gttgccgatg ctgccattac aatttccgtg tttattattc
    11281 tgattttcta tcgaaaagaa ttaaatgcaa gttttgagaa aaaagaaaaa accaatgttg
    11341 ctgagcagtc caaataaaat cttttctttc tttttgctcc tcgcgctttt tctgctgccg
    11401 gcttgcgata atcgacccaa aaatgtgttg agtaagaagg aaatggtgaa gatgcttacc
    11461 gaataccatt tgttgcacgg cactcttcag acaacaaacc aatattatac tcctgaaggt
    11521 actttgtatt ttgaatcctt acttaaaaaa cacggaatta caaaagcaga atttgattct
    11581 tctatggttt ggtatgccaa aaatcccaaa aaatttgaac gcatttatgc tgaagtactg
    11641 caaaatctta atcatctcag agaagacatc gttaagggga agtttcgctc gcctgattct
    11701 attttacccg gaatagaaat cagtaattta tggaatcttc atccgagtta tcggtttact
    11761 ccggactcgg ctcgaacgaa attgcctttt aaaattcagc agccttatct gatgacacaa
    11821 gatgtttacg aacttcaatt tttggaaaga attgctcctg aagattcctg tgttgaccct
    11881 tatgtagtaa tgaaaattca ttatgcgaat gggaaaacgg acagtatttt tcaaaaaact
    11941 ttcaacgaca gcatattgcg tcgttacacg ctccgacttc gggccagatt tccctataaa
    12001 gtcgattctg ttaccggctt gctgttgggt tgcaaacaat ataatggaaa tttcaatgtt
    12061 cagcttgaca gcattcggtt gttacgctat tttgttcctc agtggcaaga tagtttgaag
    12121 caggttgttg aaaaaaatgc agctcctcct caaaaaattc aaaccaaaac aattcggcca
    12181 aaagaaaatt gaataattcc aattttcact atccgataaa aagtaaaaag cgatcagtaa
    12241 atggtcgctt ttcattagag gaaaaaagaa caatcctcaa acccctgccc tcaacttgga
    12301 aagccaatgc tctattgacc aagctgcttg cggaaaagtt gtaaaatatt ggttgagaaa
    12361 aatatttacc aatcgatatt tgccattttt atagcggtaa tggcagcctc aacgcctttg
    12421 ttgccattga ttcctccggc acggtcgaga gcctgctgga gtgttttggt agtaagaaca
    12481 ccaaaaatca ccggacaacc tactgcattg gcatttaaat atgcaattcc gtttgtaaca
    12541 ccttcgcaga cataattaaa atggggcgtt tctccttgaa ttacacaacc caaagctatg
    12601 atagctgcat atttgtgtcg ttttatatgg tccatttctt catattgctc caacagctgt
    12661 ttagcgccat aagtcagctc aaaagtaccc gatacatgaa caatattgat taattcggga
    12721 tttgagccgt tttctatcaa ggcattataa gctccctcca acatggcatg ggtgatttga
    12781 ggattccaat cagctaccac aatgccgtat tgttgcagaa aaaccttgct tttatccggc
    12841 atttcatctt cattatagga ggaaagattt ttatattctg tggacattgg gttataatgt
    12901 aattttttat gatatttttt taaaaaaaca attttttcaa attttacccg acaaaaaacg
    12961 tcaaattagt tctgaacgcg tgccagatat ttgtcgatgt cgcctgcctc catacttttt
    13021 ggatattttt ccttaatttg aagaaaatat tcttcggcct ttttaagttg attcatcgat
    13081 tcgcaaagca gaccggcttt tttcaaataa agggggctca aaacctcatt ttcaactgca
    13141 atagccttgt tgaaataagt caaagcttta tcattctgac cgatttctac aaaacaatcg
    13201 cccaataaac caatgacaga agtagagaaa taactgttgt ttccatcaaa ttgagtcagg
    13261 tatttgatgg cattttcata ttgccctagc ttataatagc aaataccggc atatgcagcg
    13321 gccaaattac ccgaaggtgt tattccgtat tccgatacta tttgtttaaa acccataaac
    13381 tcgggttctt tcccattaag tgcaacctga aacgaatcga gattgaaata gtattgagca
    13441 tggtacattt cattttgtgc tgctttttca cgaggagtca aataaaaatt acgtactgcc
    13501 aatacaacca gaacaaccat tatcaaagct cctactacgt atagaatctg cttctgatac
    13561 ttttcgaaaa acagttcgga tgctgataaa gcatgttcca catttgccaa ctcgtcttga
    13621 ccttttccct ttttcttcga cataagaatg gttatcttta ggattttaaa tttgtgtgtt
    13681 gtttaaataa tagaatgcaa aattaaagat tttctttcgg ataatgaaat tttatggtaa
    13741 gtttttcaca tagcagactc aaccagccag tgcgaaattt acataaaatg tttgataaat
    13801 tggaattttg gtaaatttca gtttattcat gcatcataaa aaaaaccatg acttttttgc
    13861 aaatttgaat cagccgatat acaatacagt ttgaaaaacc aaacaacaac gatataaaga
    13921 tgccagccaa gaaacattaa atggttacta agattacaag gttatggata aggtttttct
    13981 tttttctcct gttttccgta ctttttcaag acacccatgt taccctatag tgggcaatat
    14041 ttttaaacat gaaaaaaatc gatttaaaaa gtaggcgctt actttttaaa tcgattttcg
    14101 tcaatgttca ttcaaccatt ttattgtggt tgtccggttc ctttcagcag aataactttg
    14161 tcgggagaaa gtccgccacc tgaaatagtc agcacagcgg tgtgttcgcc tgaagtcgtt
    14221 ggccgataag taatgttgag atttacacca tcagtgctat tggctgcatt ttgagtaatg
    14281 gaatttgccg aaacggaaaa catttctgcc tgatcgccgc tgagggtaac ggtcaaattc
    14341 ccggttattc cgttcgtttt aatgttgatt actttcaggg cagcattgtt tacggctgtg
    14401 taaggaaaaa caagttgaga ctgaataacg ccgagtttta tcacagcttt gtttgggtct
    14461 tcgatttcgc cacccatcaa cttgaaagta acttgatcag atgatttgga aatttctgtg
    14521 atagctcgat taatgtttgt tcccgaccat gttgttggtg tgaatgatcc gcttgtgaaa
    14581 gctgttcccg gagtggtggt agagccgctc aaaggttgca ggtaaactct catgtggctg
    14641 cttgctgttt gagtagttcc ggtgtagttg ttgggttcgt tgttgtccca agccgtttga
    14701 tcgtaatcga tgtgccaaat aagcattcct tcggcaggta gataagcatc ccaaccggtc
    14761 tttttacgat attcgagtac aaaaaactct tttggagagg gattatttcc taccaagttg
    14821 tgtgtagtag ccgaaagtaa atagctttgc tgatgggtgt ttgccggttg cgttgtgccc
    14881 tgataaatag gtttcagagt caggttgctt ggtgtattgg cttcttcggg agttagccag
    14941 cccaaataaa aacggtcgta agtcgaataa acgggaggcg ttcttccatc gttcaagtaa
    15001 ttaccggcat ccataatgtt ccagtagtct aatgttgttt tgtcttcggc agtgtgataa
    15061 taatcgggta accccagaac atgaccgaat tcatggcaaa aagttcccac tccacacata
    15121 tttgaaccgg aagttccttt cagttctgaa gtacaggcgt aatcgtacag ccgaacgcca
    15181 tcgaaagtaa cagaagccac tgtaccatca taagtataat cggttgaaga ttcttggctg
    15241 gtataaacta cccatcggtg tggccaaatc gtattttctg atgcgccttc cgcctcgttg
    15301 tagccggcgt aataaacgaa tatgttgtct atcagtccat cgttgtcggt gtcatattgc
    15361 gaaaaattta ctccggcatt gtcggctgcc ttacaggcat caacaatcat tcgtgccgga
    15421 tccacatcgt atccatccgc atcattggct ccgtaacttt gcattgtttt tgataaagtg
    15481 taagggccta ccacatcaaa ttctggagaa aattttccgt aagagcttgc catgaaatag
    15541 tcgcgtgccg aaccagtagc gccattggca ctgtaaccgc tttgattgag cagattggta
    15601 aaagccgatt gagccgaaga ggttacaaaa cttttatcgg aaaaatttac cagaataacc
    15661 agcgaacgtg gtgaatttga cagaggaaag gcatttttgc gagcagaaag ttgtggcttc
    15721 tgaaattggc gaccggaagc ttttacatgc tgcagggaaa cagaggaaga aattttcaaa
    15781 cttttcagaa attgaatatc ctgtgcggaa cgacttgaaa catttctggc tataacttca
    15841 cttggagtga ttctgccggc agcatcctgt gaagcatacg tccagtaacc ttttgcattt
    15901 tgttttacta aatagccatc aacagttgtt ttaatatgat gaaactcatc accatgaatg
    15961 cgaatggtaa gcacagttcc gtccggttgc gtaatctgaa caggaaatgg ataggcttta
    16021 acggcatgag tgaaactcag aggcaacaaa gcaagaaaaa aagcaaagaa aagagaacga
    16081 taacgattct tcatgggttt aatttttttg ttgaacaata aaatctgaaa ctatctaacc
    16141 tgcttattat aaacatacta caaaattttg tttccatatt ttaatttgtt tggaaaatat
    16201 tagggtggca aagataacaa taattttaaa tttaaaatgg ttttctgaaa aattgttggt
    16261 tttttcgaga aaatttaaaa tcttcatttt ctaaaataga ttgacaaaaa tacatcacta
    16321 ccgaatatat acaaaaaatt tgcctgacat aaaatgatca gctcggcatt gatggtagat
    16381 ttcttttttc aaaatttttc attcgtggaa aaggaagtct ctgaaaatac tcacagactt
    16441 cttcatcagt attctcctga aaaaacttct tttgtgaata tctctgatca ggatataaag
    16501 cgagtagaag atattttgaa caacagacca agaaaaaatt ggatttcatc agaatataat
    16561 ttatttttta atggtctgat ggaacccaca tgtctgatat attcagttgt tcgtgtttgt
    16621 gtatttaata gatgtgggtt ccttaacatc aaatgaattt ttattacgaa atttgtgtta
    16681 ccaaaaagtt gtatttacta attgaattta caactctaat ttttgtggtt gataagaaat
    16741 tttgcttatc cctatatctc tgcaaacgtt tgtattgaaa ttttatcgaa ttttcattca
    16801 aattttcctt tcattcataa aaaaataata tcattgcagc gttttattcc atcaaatttt
    16861 tcaagaataa atttataaaa aactcaacca tgttaaacat tcaaaagaaa atgagcaatg
    16921 ctttttttgc tcttctcagt ttgccttcta cagcaatggg ttttgccctg tcggtgcaga
    16981 tttcggcatt aagttggatt ttatctacca aatacggatt ggctattcat gaaataggta
    17041 ttgtttgggc agccggaccg attgcgggaa ttattgccca gccaattgtt ggggctattt
    17101 ccgataaatt gtggtttatg ggtggcaggc gtaggccttg gattgttttg ggtggaacat
    17161 tggcagcttt gatgttgctg gctttgccaa atcttgaagt tatacaaaag attattgtag
    17221 gtggaagtga tccacaagcc ggacttttga ccattgccat tgtggtagca ctcacgctcg
    17281 atttgtccat caatgtcagt tttaatccca cccgttcact ggtagccgat tgtacgcctt
    17341 tgtccgaaag aagcaaggga tttacttgga tgcaaactat ttcaggttct ttcggcgtgt
    17401 tggcatacct tattggcgga acgctgggta atatttttct tatatatttt ggtgttggcg
    17461 ttgcctttct ggcttcggtc attccggctt ttcttatcga agaacctcgc gaattgaaaa
    17521 attttgatgc tgaaactcag tctcccaaat caaaaacaac tttccgtgaa ttgtgggagg
    17581 caatgcttcc gctttccgga tttttacttt acggtattta cgtgattttt tcaaaattgg
    17641 gcggatttga attgaaacca accatccctc tttttggtta ttatttgaat gttatcgaat
    17701 ttttatctat tctgcttttg ctggtaagcg ccattgctgt tttgctgaat cttttcaaat
    17761 ccggaaagaa aagcaacgag tttcaaaaag tcttgctggc acattctttt acatggatag
    17821 gcgtgcaaac gatgtttatt tatgctcttt tttatttgaa agatgttttg caattggcag
    17881 ataatgcagg ttcggtcaac tccattgcgt tttttgtttt aagtttggta agtgccgttt
    17941 taccggtttt ggtattgaat cggcttgcgg aaaaatatgg tatggtaaaa acacatttgt
    18001 cttgcatttt tatcatggca ttgggatatg catccatttt atttttcgga cacagtgttg
    18061 ccgtatatta ttttctgata gccatagtgg gaattggctg gggatcagtc gtttcgttgc
    18121 cttttgccat tatgagcgaa aaggtggatc aaaccaaaat gggactttac atgggaatat
    18181 tcaatcttgc cgtagtgttg cctcaattgg tggcaagttt caaaatgggt gaagtaatca
    18241 acgctgctcc taataagtcc gtcatatttg ccatttgcgc cgtaacactt ttcatttcag
    18301 gtatattgtg gttgtttgtg aaaaatgaaa agtaagtttt tagcaatttt tcttttttca
    18361 agagcaatgg atttatgttt ttttgaatta atatttgaaa agtaaacaga aaatatttac
    18421 ttttgcgcta aaagtaaatc gacgtctaaa gatgtagatt tacttttttg tttatttaat
    18481 cattatattt taaaaccaat aatcatgttt ctaaacattc cactcttaga atggataggc
    18541 tatctggcat ccatcatagt tgctatttct ctgacaatga gttccataaa aaaacttcga
    18601 tggttgaatc tcataggttc tgccgtgttt tccttttatg gattttctat tggagcttta
    18661 ccggttggat tccttaattt gtttattgta ctggtaaata tttattatct gatcaggatg
    18721 aattcggctc aagaatcttt caaagcactt gccgttgaac caaacgatgc ttattttaat
    18781 tattttatcg aattccataa aaaggaaatt ctacagattt ttcctcattt cgagcttgct
    18841 tcaacaaaac aagataaaac ttcttccata gctttacttt tgttgcgtaa cgccgttgct
    18901 gccggagttt tttatggaac gatcgacaat ggttgtcttt atgttgagct tgattttgtt
    18961 gcttcagaat atagagatct gaaaccggga gaatttatct acaaaaccaa tgtgcaacag
    19021 ttgaaggaaa ccggcattca taaacttgtt gccaaatcgg aacatccgca gcaccataaa
    19081 tatttgataa aaatgggttt tgttcgtcgc aatgatacac cggctgtttt cgaaaaggaa
    19141 atttaatctt gtttaaaata atctccagat aggttcaaaa gtaagctctt taatagaatt
    19201 ttctttcttt tcaactcggc acatgcagga actctcatcc tattagctta ataccatgcc
    19261 gacatactaa aaaagaaagt gtccttttgg gacactctct tatttgataa tgtttaactt
    19321 aattagttcc tgggcttttc ttcttttggt ggacgtggaa gaagagcttt ccgtgacagt
    19381 ttgaatttac ccgtcttttc atcaatatcg agaagtttca cttctatcat gtccccttcc
    19441 tttataccgg tttggtccat gctttcagta cgtttccatt caatctccga aatatgcagc
    19501 aaaccttcct tgccgggtag aaattctaca aaagcaccat aggacataat ggattttact
    19561 tttcctatat aagtttcacc aacttcagga atagcaacta ttcccttaat tttgtttatg
    19621 gcagcttcaa ttgagtcctt attggtagca gcaatttcca cacgtccctg atcgccaatt
    19681 tcttcaattg tgatggtagc acctgtttcc agctgaatgt tttggataat ttttccgccc
    19741 gggccaataa ttgcgccaat catttctttt ggtacaaaca ttaccacaat gcgcggaaca
    19801 tgcggtttga agtcggcacg cggttcagat atggtttcca acattttgtt caggatatgc
    19861 aatcgtcctt cttttgcctg tctcagagct ttttccaaaa tttcgtacga aagtccatca
    19921 actttaatat ccatttgtgt ggcagtaata ccttctctgg tgccggtaac cttaaagtcc
    19981 atatcgccta agtgatcttc atcacccaaa atatcggaaa gcacggcata attttttccc
    20041 tgattttcag aaatcagtcc catagcgata ccggcaacag gttttttgat ctgcactcct
    20101 gcatccatca gtgctaaagt accagcacaa acagttgcca tagaagacga tccgttggat
    20161 tccaaaatat cggaaacaac gcgaataaca taaggataat cgtccgggat aacatttttt
    20221 aaggctctca tggccaggtt gccatgtcca atttcccgac ggccaatacc ccgcgaagcc
    20281 cttgcttcac ctgtggcaaa aggtggaaaa ttgtaatgaa gcagaaaacg ttcttttccc
    20341 tgaattaaag gttcatcaat caacttttcg tccattttgg ttcccagtgt tacggtagcc
    20401 aatgcctgag tttcacctcg cgtaaaaatg gaagaaccgt gcgtccccgg caaataatct
    20461 acttccgacc atattggacg gatttcggta gttttacgtc catccagacg ctttccttca
    20521 tcaaggatag cccgacgcat ggcttctttt tctatggcat gataatatct ttcgatcagc
    20581 ggtttttttg cttcggctgt ttcagtatcc aacgatgcaa tatattcgtt cataatggcc
    20641 tcaaaatgtt cgctgcgcga atgtttgtcg gcatttccgg aagctgcttc ggcataaacc
    20701 ttatcgtagg ttttttccca aatgtccttt ttcagctctt cgtcgttttg ttcgtggcaa
    20761 tattcgcgtt ttttagtctt gcctactgtc tccattaatt ccaactgagc tttgcagtgt
    20821 agttttatgg cttcatgagc aactttcatg gcatcaagca aatcgtcttc gctaacttct
    20881 ttcatttcgc cctccaccat catgatattg tccatagtag cagccacaat aatgtccata
    20941 tcggcatttt ccaactgatt gaaagttgga ttcactacga aatttccatc aatgcgtgcc
    21001 acgcggactt ccgatacggg cccgttgaaa ggaatgtcgg aaacggcaag tgcagcagaa
    21061 gctgccaaac ctgcaagcgc atccggaata ttttccccat cagccgaaca aagcataatg
    21121 ttcacgaaag tttcggcatg ataatcatct gggaaaagtg gacgcaaagc cctgtcaacc
    21181 aagcgggaaa taagaatttc atagtcagaa ggacgacctt cgcggcgtaa aaaacctccg
    21241 ggaaaacgtc caaaagcagc gaatttttcc ttgtaatcta ccgataacgg cataaaatcg
    21301 gttccttcat tggcatcctg agccgaacat accgttgcca gcaacatggt atcacccatg
    21361 cgtaccatta ccgatccatc agcctgcttg gccaatttac cggtttcaat aacaatttct
    21421 cgtccatcac tcattgtgat ggtttttgta acaactgtca tacaattttt tcttttaaac
    21481 ttttattttt cggatacaaa gatacaattt tatttgcaaa ttttttaatc cttaataatt
    21541 tacagtcata agtaacttat agtaacgtaa attcaagtaa ttaatgcaac tttttcaggg
    21601 gaaataaaag gtaaagaaaa attttttctc aaaaacaagg gaaaaaataa aaggggtatc
    21661 taattcggaa aaaattttga tacccctttg ttatttatat tggcatagaa caaaaaatca
    21721 aatttctttc accagttctt tcattatcag tgtcataagt ggctgcaccg aatctccgat
    21781 tttttgaact tcttcgtgag aaacttccac aatttttccg ggtacaccca aatcggtaat
    21841 aatggaaata ccgaaacatt tcattccggc atgattggcc acaattactt cgggaacagt
    21901 gctcatgcca actgcatcgc cgccaatggc acgaaaatac cggtactcgg caggagtttc
    21961 aaaagtaggt ccactggttc ctacataaac gccttccacc actttaatgt gatttcgttc
    22021 ggcaatttgt ttggccttta caataaaatc cttgcaataa gcctcactca tatccgggaa
    22081 acggggcccg agctcttcaa aattctttcc tcgcagtgga tgttcgggaa acaaattgat
    22141 gtgatcggta ataatcatca aatctccaat ttcgaaatcg ggatgcatac ctcccgcagc
    22201 attggaaaca agtaaatttt taatacccag tgctttcatc actcttacgg gaaaagttac
    22261 ggctttcatg tcgtaccctt cgtagaaatg aaaacgtccc tgcatggcca acacttctac
    22321 tcctccgagt tttccgagta tcagcttgcc tttgtgtccc tcaactgttg aaacgggaaa
    22381 attgggaatg gtttcgtaag gtatttccat ttcaatatcc atttgggtta ccaaatttcc
    22441 caatcctgtt cccaaaatga tacctgtttt aggtcgcatt ggcatttttg ccaacaaaaa
    22501 atcagcggtt tcttgaattt tctctaacat aatctttaag tttttgaatg aataatgatt
    22561 cttgatcgaa taaaaattta acctctatcg gcaaggcaaa aatttttgcc ttgagcgttt
    22621 caggtaaata tttttcgtta taaagtcgtg cggcatcttt ttccgtaacg ataataattt
    22681 tattttcgga cggaagggcg tcccattcct gtcggatcaa ttggaagtca tttttggaaa
    22741 atttgtgatg gtcggggaag gaaacttctt tcaaagtcga aacataatct ttcagaaaat
    22801 ccttcatcga ttgaggagaa gcaatgccgg taaccatcaa aacggccgga ctttcttccc
    22861 tcaatttgct caacgaccaa ttttcagtag tcaactccgg aaaaaccggc accggatcgt
    22921 gataccgaaa agtagaaaaa aacacttgct gataaggcaa tggatgcagc tccatttcaa
    22981 taatgcgttt gtcgatgggt ttgaggtttt ccgggcattt ggtaaccact atcaggttgg
    23041 cacgatggct gccttccttg ctttcgcgca aactgccggc cggcagaaaa aaatcggatg
    23101 tgtaaaggtt cgaataatcg gtgagcaaaa cggaaaaacc cggaagcaaa taacgatgtt
    23161 gaaaggcatc gtccaacacg atcaattgca aatcgggaaa aagtttcaac agacgtttga
    23221 tgccctctac tcttttttcg tccactgcca ccttaacgtt gggaaatttt cggaaaattt
    23281 gataaggttc gtcgccaatg gtagcggcag aagcattttt gtccgccaaa acaaaacctt
    23341 tggtttttct tttgtatccc ctgctgagaa aagcagtttt aaattcatgc tgcaaatgcg
    23401 acaaaacaaa ttctgcatgt ggcgtttttc ccgtcccgcc aacagccaaa tttcccaaca
    23461 caatggttgg cacatcgaac ccgacggaat gaaaaatttt ttcgtcaaac atccagtttc
    23521 gaatatctgc tacccatccg taaatgaggg aaaaaggata taaggcaata tgtttgagct
    23581 ctctgttcaa attggcaaaa tttttctgaa aaagaagaga gcaaagatac catttttctg
    23641 atttgaaaca aacttaaacc gacaaatcat tgacaggtaa aagaaagggc aaataattaa
    23701 ccacaaaagc agccagcctg caatttgccg gagcaagaga attttgattt gctttcaggg
    23761 aacagggtcg tagccactgc ctccccaagg atgacaacgg ctgatgcgct tcacggaaag
    23821 ccatccgcct ttgaaaacgc catatttttt tattgcctcg atggtatatt gtgaacaggt
    23881 gggtgtgtaa cggcaacttg gcggaaaaag cggagaaatg ctgtaccggt aaaaatatac
    23941 cggcaacaaa accaaccatc gaaaaaattt tttcattgct cagaagctat tttttgcaac
    24001 tgttccaaaa tctgtttcat tttatcattc agctcgtgga agttaatttc cttgtcagcg
    24061 acataaagaa atgcaacttg catttgccgt tgttcggata aaacaacatc ggaaagttcg
    24121 tttttgttca aacgataggc ttcgcgtatc agccgtttaa tgcggttgcg atgcactgcc
    24181 cttttaaact tttttttggg aacggaaatc aggacttcta ctccggcagg attttcagca
    24241 gagacataat aaaccgtcct cagaggataa accagaaaag cttttccttc ggtgaacaag
    24301 cgatctattt ttattttgct gttcagtcgc tccgatttgg ggaataaaaa tcttgttctc
    24361 tccattt
//
LOCUS       DAOSIB010000028        22763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_2515_length_22763_cov_10.529461, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000028 DAOSIB010000000
VERSION     DAOSIB010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 22763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22763
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2515_length_22763_cov_10.529461"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..985)
                     /gene="corA"
                     /locus_tag="PLD44_04110"
     CDS             complement(35..985)
                     /gene="corA"
                     /locus_tag="PLD44_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOF98551.1"
                     /translation="MSELRIFYHENDGKLKISKSIDTLKKINLKDIIWIDMNDVSENI
                     ESELEQFLKIYIQEDEEIEEIEMSSRYIQTDDSIVANSNFLQDNYVMQPVSFIIKNGI
                     LVSVRNTELKSFNETVKKIFVNTSNFPSGYHVMVSLFETRVEYDADMIEDVTDQITNL
                     SKNLNTESDLDQDILLQIKELQEKVMIIRQNIVDKQRVVSNMLKCNFFPEDLIPRLNI
                     ILKDINSLFEYTRFDFDRLDYLQDTFLGLVNLQQNNIIKIFTVVSVIFLPPTLIASMY
                     GMNFKYMPELDWKFGYPFSILLMISFTVGVLVFFKRKKWL"
     gene            complement(1058..1840)
                     /gene="rsmA"
                     /locus_tag="PLD44_04115"
     CDS             complement(1058..1840)
                     /gene="rsmA"
                     /locus_tag="PLD44_04115"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOF98552.1"
                     /translation="MTLVRPKKYLGQHFLKSMDVARRIAGSLPVGSPASVLEIGPGMG
                     MLTQFLLQQPQIDLKVVEIDHESVVYLQQHFPQLQILEKDFLQLDLNQIFSHPFSIIG
                     NFPYNISSQIFFKVLDNKDLIPSVVGMVQKEVAERLASPPGNKTYGILSVLLQANYDI
                     NYLFTVDETVFYPPPKVKSAVISLKRKENPEVDYDEQLFRRVVKTAFNQRRKTLRNSL
                     KSIIPFHPMNADPLFDLRPEQLDVKTFVELTNKIGSALKEKQ"
     gene            2105..2548
                     /locus_tag="PLD44_04120"
     CDS             2105..2548
                     /locus_tag="PLD44_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOF98553.1"
                     /translation="MPLQFFRYGVSGAANMVFDWVLYFVVYNFVLRHQMLHLGFITMS
                     SHIAALALTFPVSFMTGFLLQKYVTFSASYLRGHVQLRRYLLVVIINLGLNYAGLKFF
                     VEIAHFYPTPSKMLTTIITTLFSYFAQKKFTFKTASLSLNKESNH"
     gene            2582..3574
                     /locus_tag="PLD44_04125"
     CDS             2582..3574
                     /locus_tag="PLD44_04125"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOF98554.1"
                     /translation="MAVTTKMTTDKSKLWNIFKWSCLTAAFLFLGYRLICFQHYGRLL
                     SQFKEISFWKTWYLWGVILLLPANWLLESMKWQRLVRNSEKINMALAFQSVMAGFLTA
                     FFTPNRVGELAGRIAYLQPENRKAGITLSFVNSITQNIIMAICGIPAAILFFLSNKNS
                     EFAGENNFFGYFLIVCGILLLLSGIYFSLPPLVGRIKQKKLSAKWINFIACLADYSLA
                     DLLKILLLSFLRYLVFSFQFYLMLRFFGVEINGLAAAIAIPTTYLFVTFTPSMAFSEA
                     AIRSSYSVLVIGAFSGQTVQIILASTFIWVINFVIPMFVGSFVMMQTHPLTSNK"
     gene            complement(3584..4432)
                     /locus_tag="PLD44_04130"
     CDS             complement(3584..4432)
                     /locus_tag="PLD44_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HOF98555.1"
                     /translation="MKTIQNTAILVFLLAFNSILYGGERIKLTILHTNDTHSQIEPTE
                     KSNLPVSDKGGYARRMGIIEQIRQQNKNVLLFDAGDFWQGTPYFNFYNGRIEVDGMNK
                     MKYDAVTLGNHEFDNGLDTMATVLKNAQFPILTSNYQMDSTAMATLVKPYLIIRKAGL
                     KIGVMALNVNPKGLIFTNNYKKMNYIDPVETAINISNFLKRSEKCDLIICLSHLGADT
                     LKQTLTDYDIIRKTRYIDVMIGGHSHQIITQTNLKNAAGKPVVLAQMGKSGLYLGRID
                     LELEKK"
     gene            complement(4445..5200)
                     /locus_tag="PLD44_04135"
     CDS             complement(4445..5200)
                     /locus_tag="PLD44_04135"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HOF98556.1"
                     /translation="MKVNFCTLFVLFNILFYSCSPKTWQVVEATSVKIPIDSTTEKFA
                     DKEYEAALQPVKERIDAEMAVVIGYSAENMRSFAPESPLSNFCADVFRRAASDFLHQE
                     VDVSVVNLGGLRTEIPAGNITIRNIYELMPFENELVILWVRGDKLIQLFQFFASVGGE
                     GVSGISMKIKNGKATQILVGTHPIDPEKIYTVATSDYLAEGNDQMTALSEYEKISGTG
                     LKLRNVLIDFIRNETLKGHVIQSKIDGRISMEN"
     gene            complement(5356..6420)
                     /locus_tag="PLD44_04140"
     CDS             complement(5356..6420)
                     /locus_tag="PLD44_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HOF98557.1"
                     /translation="MLQIFQPFRIKDITLKNRLGMSPMCQFSAQDGLANDWHLVHYGT
                     RGVGGIALIIFEATGVSPVGRISPEDLGLWKDEQIEPLKKIVDFIHQQDSKAGIQIAH
                     AGRKGSRSSPFKGEKQLTNEEGGWQTIAPSAIPFRPEEIPPHELTKSEIKKVVNQFKD
                     AALRAFQAGFDVLEIHAAHGYLIHEFLSPISNFRKDEYGGSFKGRIRLLQEIVSAVQT
                     VWPERLPLFVRLSATDWTDQGWRLEDTVELAKILYLEGVDLIDCSSGGILPNVHIPLA
                     PCYQVPFSEQIRKTGILTAAVGLITKPEEIQDILRTEKADIVLLGRELLRNPYFALHC
                     ARHVKENSKWPQQYLRAKLE"
     gene            6658..8130
                     /gene="rpoN"
                     /locus_tag="PLD44_04145"
     CDS             6658..8130
                     /gene="rpoN"
                     /locus_tag="PLD44_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOF98558.1"
                     /translation="MLKQQLQQKLQQKLSPAQIQVIKMLEIPTLELEERIRQELEENP
                     VLEIGLEPEEEKKELDDFGEDYEETGNDEGESNDEIDFEEYMSDDDIPDYKLKANNTS
                     KDDKYEEIPFSAGSTFHDFLMEQLNLVPLSERDRALAEFVIGNIDEEGYLRRTPEALA
                     DDVAFQANMDTNVKEIEKIVKIIQQFDPPGVGASSLQECLLLQLEKKRNEKEIEIPKR
                     IIQDYFEEFSKKHYDKIINGLEIDGNTLKNAINEIIRLNPKPGNAWSGNILEKNMSII
                     VPDFIVENNDGELTVHLNNSNIPYLRVNAAYSEMFQDYTSNKENQTPQMKEAILFVKQ
                     KIDSARWFIDAIKQRQQTLLTTMTAIVEFQKDFFIEGDEIYLKPMILKNIADRTGYDI
                     STISRVSNNKYVQTEFGVFPLKYFFSEAMTNDAGEEISTREIKKILQECVDNEDKRHP
                     LNDEMLAEVLKQKGYLIARRTVAKYREQLNIPVARLRKEI"
     gene            8169..8774
                     /locus_tag="PLD44_04150"
     CDS             8169..8774
                     /locus_tag="PLD44_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98559.1"
                     /translation="MKILYKIISIILQPILLPTYGILLLIQIPVFANLPMQWKAIAVG
                     GTFLFTALLPSIPVVIMYFRKEVSDLFISNREERTLPYLFSFLAYVFWTLFLVRTMHL
                     PMFIGGLGIGSSISILLITFINVKWKISAHLASLGGLAGGIFGICYRYSINPLWLFIL
                     ILLLAALSAISRVALKAHTPMQTIAGFALGFLCVFLPVLLL"
     gene            complement(8797..10836)
                     /locus_tag="PLD44_04155"
     CDS             complement(8797..10836)
                     /locus_tag="PLD44_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOF98560.1"
                     /translation="MHKNFFFYLIEILFLFFFIPVRAEILFQDMGMDTAKTYSIPEIV
                     VVEKFQNSEIRSAMPLQILSAKKLENLNVLQLSDAIKYFAGVNVKDYGGIGGLKTVSV
                     RSLGAEHTAVSYDGIMLTDYQTGQIDLGRFSLDNVDMVSLNNGQSDNIFLPARSFASS
                     SVLNIRTLPPRFEEEKSIHGKITCKVGSFGLINPSLFIRQQMSRKISAVFSGEWLTAE
                     GEYPYLLIYGTQTGDSSSIETRKNTDVETLRLEGTIFANFFHRGSGYLKTYFYESERG
                     LPGAVIYYMNENTNHQRSKDQTFFTQAHYESSFSSLLSFQANTKYQNGYLHYVDPNYN
                     NSGGKIDNTYSQQEFYFSASFLYKAFENLSFSASTDYSLNTLNANLPEFAFPKRNTVL
                     MVLNAKYVSNQFLATAGGLQTFVNEKVKFGSSPNDKKHFSPFFSLSVKPFEQYDFRIR
                     SFYKNVFRMPTFNDLYYSYVGNRNLEPENAQQFNAGITWSFSSESISLLSVTLDAFHN
                     AIRDKIVAFPNKDIFNWTMKNCGKVSINGLDVAMESGISITRNIGLVFGGSYTYQQAL
                     NVTDPNAEDYLHQIPYTPRISGSGKAALEMPWVNLSYSFIWLGHRYTLFQNYASNRLP
                     GYADHNLSLSRSFKKSSYSCFFKLEINNFLDKNYEIVRGFPMPGRSFRSIVAIEF"
     regulatory      complement(10937..11177)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(11442..11573)
                     /locus_tag="PLD44_04160"
     CDS             complement(11442..11573)
                     /locus_tag="PLD44_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98561.1"
                     /translation="MNIIFRHVDSIRPLKNKLYSDENGQSQSDGSMRGITGKGMSRV"
     gene            11926..12996
                     /gene="gcvT"
                     /locus_tag="PLD44_04165"
     CDS             11926..12996
                     /gene="gcvT"
                     /locus_tag="PLD44_04165"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOF98562.1"
                     /translation="MKITPFTHFHIALGAKMTEFAGFYMPIEYTGINEEHLTVRRAAG
                     VFDVSHMGEFLVKGPHALAFLQKMTTNDVAALPLGKAQYSCFPNGRGGIVDDLLVYRL
                     DKENYLLVVNAANIEKDWNWCMQNNTENAELVNISEQVAQLAVQGPKATQILQKLTPV
                     RLSEIPYYSFAIGKFAGVEPVLLSNTGYTGAGGFELYFRPDCADTVWNAIFEAGADEG
                     IRPIGLGARDTLRLEMGYCLYGNDIDDTTSPIEAGLGWITKFVEGKNFIDRPLLEKQK
                     REGVTRKLVRFELTERGIPRHGYPIVNNENKPVGQVTSGTMLPDSKKGIGMGYVATEY
                     AQPGTKIYIQIRNKNLEAIVRK"
     gene            complement(13071..13538)
                     /locus_tag="PLD44_04170"
     CDS             complement(13071..13538)
                     /locus_tag="PLD44_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding protein"
                     /protein_id="HOF98563.1"
                     /translation="MRINKFFILSILSLALFSCEPKYEKEYNWAYPVCGDWMVKAYLD
                     GTEIYGPFEIMTYNTSFGQDSIWIHDYATTATNGHFWSFKVKAAVDMKTLTFQTEGSI
                     NAIAGYPIVIKVSNGRVINKDSITMELEFEDDPGTIYQLAGHRETSYEEYMQQ"
     gene            complement(13552..14262)
                     /locus_tag="PLD44_04175"
     CDS             complement(13552..14262)
                     /locus_tag="PLD44_04175"
                     /inference="COORDINATES: protein motif:HMM:NF027719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5012 domain-containing protein"
                     /protein_id="HOF98564.1"
                     /translation="MKKIILMTLAFVAFVFTSCEKESLGVSRVTTYATMELNGPEELF
                     WPLNSTFVDPGCKAFEGTTDISGNIEVISNVNATKGGKYSITYKVKNSDGFYAQTSRT
                     VYVYEPSAPLNGYYQSRIRRNNNGTIANRGPFTILVFGVGNDQYWIEDLLGGWYNYGS
                     GYGPAYAGPGIIKLNSDNTFEIVSAERLAWGYPCILTENTVSTYDPSTKTIVLNTRME
                     DTPKMLFTVTLTNPQPLN"
     gene            complement(14291..15688)
                     /locus_tag="PLD44_04180"
     CDS             complement(14291..15688)
                     /locus_tag="PLD44_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOF98565.1"
                     /translation="MKRLKFYNIAAFIGLLFLSSCSESFFDINHDPNNPSEATPGLVL
                     PSALANSAYVMGGYYHALGSFWTQQYAQAPAASQWADWESYNLTNVDFDRQFILLYAG
                     GLNDYEYVRKNTAETGNWKFYAIATLMQAYTFQVLADLYDQIPFREALKGNVIPHYDN
                     GQLVYDSLLARIDYAMSKDFTGSTVENPGSSDLIFGGNIDDWKAFANTLKLKMYLRYV
                     NVNPNTYRTEIEALLAENKFLTKDAKFTAFKEEQTGYNPFYNTFVDRLSGNVVANKTF
                     IDLLKDKNDPRMAKIFNPSVTGSLYNGIAAGDNKNHPTETSKNYATPAIKGTSPVYFF
                     SKEEVLFLIAEAQARYGTASDAESTYKTAIRASLTSLGLAADAIDIDATYPYNGIRSI
                     IEQKWIAQANKNGIEAFFDYNRTGYPNFFTRSLTSVLNGDERPKRLYFPSREVNSNPY
                     TPARVDLTEPVWWAK"
     gene            complement(15711..18863)
                     /locus_tag="PLD44_04185"
     CDS             complement(15711..18863)
                     /locus_tag="PLD44_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOF98566.1"
                     /translation="MRKLTFLFACLFMIGVGLVNAQSLTVQGKVISDEDGQPVIGASV
                     LVKGTTTGTITGTDGSFSITVPVGNRTLVVSYIGMKSVEVPAENGMVVRLQPQTLEID
                     EVVVTAIGIKRSEKALGYSASAVSSQDITMTGNRSALNALQGKVAGVEITSASGAPGS
                     STRVIMRGYSSLGGSNQPLYVVDGVPISNGQIGSTSINGGLDFGNRANDINPEDIESM
                     TILKGGSATALYGSRAASGVILITTKKGEGLKNKPKVEVSSTSTFDSPLRLPYFQNEF
                     GQGWYDRGGGEQDLMENGSWGPRFDGKIRPWGFVVDNQQQIKPYVALPTNIKDFFDIG
                     TNYNNTVSVSNGDADKSYYISYGNVTADGIMPTDADYYNRNTVSMRGSAKFLKIFNIS
                     GSLNYVRKDMRFTETGQDQAALDGLWQSPRDISIVDLKDYNNKFNNVDNYYTIYAQNP
                     YYILNEHGNKFQENRIFGNISLDAQITPSLSATFRLGDDVANSTLKTWRAITNSARAD
                     YNDEVGRVGENAYYSSELNTDFFLNFNPKLNNDFAISVIVGHNFNQRDAQSQGSEVIG
                     LDIPKFYNLSNSSATPSIYGTLSQRRLIGVYGNLDVSFKDMLFLNITGRNDWSSTLPA
                     NNRSFFYPSISGSFLFSELIQNKDVLSYGKLRASFARTGKDADPYLIYPVLLQTSITD
                     GYRSLDFPLAGPVNGFTISNVIGNDQLQPEISQDFEIGTNLKLFKNLLDIDFTWYNKK
                     ITALIWTATLPSSTGYTSQTQNLGEITNKGVELSLSVTPIRRKNIEWEIFANYSKNNN
                     LLKSLISGLDEIVLGGTGGFPFVARPGYELGLFKGNVPETDPQGRIVVNAQGLPVFKA
                     EQEIIGSSQNKYRLGGGTSLKIKNFRVHAAFDYRNGGKMYSRTAEIAYFTGNAQPTTY
                     NDRQPFIIPNSVQLVGGQYIENTTPIAGFTNNLNLYYNQTYKAGIGDAYALIDKTFFK
                     LRELSISYSFPKSVLKNAFINSVDLSLVGNNLFLWTPDSNLYTDPELTTFGNDLAADY
                     GEFGATPSTRSLGFNIKLSF"
     gene            complement(19895..22357)
                     /locus_tag="PLD44_04190"
     CDS             complement(19895..22357)
                     /locus_tag="PLD44_04190"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOF98567.1"
                     /translation="MKRFFFSFFLSILLHTGYSQFNDSLFSYSCSKNTVVVQNDNSII
                     KVEAWCDNIFRISWFPDMHNIEIDSSHSVILNQAPEVKFSVKEKHNELIIKGRLIVAK
                     ITKIPFNISFYKPDGSILLQSAGAFECDGSSKKASFKIRETDHFYGMGQKSIAVDRRG
                     EAFEVFNQHIGGYTKPYGTMQINIPYIYSTTGFGVYFDNQYPARFDLGKTNPTEWYYE
                     VENGSFTYYLVAAPNEQSLLKSYFDLTGYPPLQPKWIYGLIQSKCSYENDTMVYNTLQ
                     KFEQRKLPVDAIVLDIPWFGGLKPTSPHFMGNFSWNLQNFPDPIKYINTLRGKNVKTI
                     LINEPYINIDSYNYKFLKEKGWLVSKKNQSEPYVFSPFWAGDVSLFDITHPDAQKWLW
                     QQLKAEVEFGADGLWSDLVEPESPVVDGQFYLGDWRKVHNIYSLLWAKNIYDGFSTDF
                     PNKRITNLSRAGFGGIQRYGALCWSGDASKTWFALKLQIPMMTGTVLSGIPYFSSDIG
                     GFTFAHDRRDGNTIFTNIVDGRMKTEPELYIRWFQHGVFSPMLRPHSGEEQFCEVFAF
                     DSLTESITTKYLHLRYRLIPYMYSYSYKTYKKGETLIRPLFFEFSDSLARKQDYQYMF
                     GKEMLVAPVLDEGKTNQQVYLPSENQTWIDFWSNKTYRGGQTIDYNAPLDIIPVFVKA
                     GSIIPLAKNKMRAEEPDDTLTLRIYPDKTAKFEIYEDDNKTKNYQKGEFGITNITSWL
                     CNHQLTINIDAMQGEFENKITERTWIIEIPVSAECKSIKLNNKKLTNFTFDKKNGMLH
                     FEFKADTRVKQKIQIDKFRTTY"
BASE COUNT         7118 a         4296 c         4068 g         7281 t
ORIGIN      
        1 atattttcga caaaaaattt tatcatcttg ccgtttacag ccattttttc cttttgaaaa
       61 ataccaagac tcctaccgtg aaggaaatca tcaataaaat ggaaaaagga tacccaaatt
      121 tccaatcgag ctcgggcatg tatttaaaat tcatgccata catgcttgca ataagcgtag
      181 gaggaaggaa tataacggaa actaccgtga aaattttaat aatgttgttt tgctgcaaat
      241 ttaccaaccc gagaaatgta tcctgcaaat aatccagtcg atcgaaatca aaacgggtat
      301 attcaaacaa agaattaatg tcttttagaa tgatgttaag acgcggtatt aaatcttcag
      361 ggaaaaaatt gcatttcagc atgttggaaa ccacacgctg cttatccaca atattttgtc
      421 tgataatcat taccttttcc tgtaattcct ttatttgcag taaaatgtct tgatcgagat
      481 cggattccgt attcaaattc ttacttaaat tggtgatttg gtcggtaaca tcttctatca
      541 tgtcggcatc gtattctact cgcgtttcaa agagagaaac catcacatga tagcctgaag
      601 gaaaattact ggtattgaca aagatttttt ttacggtttc gttaaaggat ttcagctcgg
      661 tattgcgaac cgaaaccaaa attccgtttt ttatgataaa cgaaaccggc tgcataacat
      721 aattgtcctg caaaaaattg gaattcgcca caatgctgtc atcggtctga atgtagcggg
      781 aactcatctc aatttcttca atttcttcat cttcctgaat gtaaatttta agaaattgct
      841 cgagttctga ttctatattt tcggaaacat cattcatgtc aatccaaata atatctttaa
      901 ggttgatctt tttgagcgta tcgatactct tggaaatttt cagtttccca tcattttcgt
      961 ggtaaaaaat tcttaattcc gacataattt tgctcctttc tttttttctg ctgtttcgaa
     1021 gcagcagtgg tggctatttt gtcaccggat aaacgaatta ttgtttttct ttcaaagccg
     1081 aaccaatttt attggtcaac tcaacaaatg ttttaacatc caactgttca ggccgcaaat
     1141 cgaaaagagg atctgcattc atgggatgaa agggaatgat tgatttcaat gaattacgca
     1201 acgtttttct tctttgattg aaagccgttt tcacaactcg cctgaatagt tgttcgtcat
     1261 agtccacttc gggattttct ttgcgcttga ggctgatgac agccgatttt actttaggtg
     1321 gagggtaaaa aacagtttcg tctacagtga aaagatagtt gatatcgtaa tttgcctgca
     1381 gcaaaacact tagtattccg tacgtcttat tgccgggtgg agaagccaac cgttcggcca
     1441 cttctttctg aaccataccc acaacagaag gaattaaatc tttgttatcg agaactttaa
     1501 agaaaatctg actggaaata ttgtagggaa aatttcctat tatgctgaaa ggatgagaaa
     1561 aaatctgatt taagtcaagt tgaagaaaat ctttttctaa aatttgaagc tgggggaagt
     1621 gttgctgcag gtaaacaacc gattcgtggt caatttctac aactttaaga tcaatttgtg
     1681 gctgctgaag caaaaactga gtaagcatac ccatgcccgg ccctatttcc aatactgaag
     1741 caggactgcc gacgggtaaa ctgccggcta tacgacgggc aacatccatg cttttcagga
     1801 aatgttgacc caagtatttt tttggtctta ctaaagtcat tactgtgata atcgtcagac
     1861 atatgagtat ttaactgaat aaataaaaat gagaaaaact aaaaaatgga atttaaatta
     1921 attcgtatct ttgtagaaat tttttcgcca caaaattaat caaaaattgt ttgataattt
     1981 tatttataaa aaaaaattgc ccgaattgaa catttaattg ttgttaatga gaaaattttt
     2041 tcgaaacaca ggaagctcga ttacaaaatt aatcgatttt ttttacccgc ctttccgtaa
     2101 attcatgcct ttacagtttt ttcgctatgg agtatccgga gctgcaaaca tggttttcga
     2161 ctgggtattg tattttgtag tttacaattt cgtacttcgc catcaaatgc ttcacttggg
     2221 ttttataacc atgagttcgc atattgcggc acttgctctg acatttcctg tttcgttcat
     2281 gacgggtttc cttttgcaaa aatacgtaac tttttctgct tcctatctgc gaggtcatgt
     2341 tcagcttcgc cggtatttgt tggtcgtcat tataaatctg ggcttgaatt atgccggttt
     2401 gaagtttttt gtcgaaattg cccatttcta ccctactcct tctaaaatgc ttaccacaat
     2461 tataacaaca cttttcagct attttgcgca gaagaaattc actttcaaaa ccgcttctct
     2521 ctctttgaac aaagagtcga atcattaaaa caacattttc ttgtttcaac ctatgaaaaa
     2581 catggctgta accactaaaa tgacaaccga caaatcgaag ttgtggaaca ttttcaagtg
     2641 gagttgcctg acggcagcat tccttttttt gggttaccgg ctgatttgct ttcaacatta
     2701 cgggcgattg ctttctcaat tcaaagaaat atcattttgg aaaacttggt atttgtgggg
     2761 tgtaattttg ttgttgcctg ccaattggct attggaatcg atgaaatggc aacggttagt
     2821 gagaaattcg gaaaaaataa atatggcttt ggcttttcag agtgttatgg cgggatttct
     2881 tactgccttc ttcaccccga atcgtgtggg agaactggcc ggcagaattg cctacctgca
     2941 gcccgaaaac aggaaagcag gaataacatt aagttttgtt aacagtatta cgcaaaacat
     3001 cataatggct atttgcggta ttccggcggc cattttattt tttttgagta ataaaaactc
     3061 tgaatttgca ggagagaata attttttcgg atattttctg atagtttgcg gaattttgtt
     3121 gttattgagt ggaatttatt tttctctgcc cccattggtt gggcgaataa aacaaaaaaa
     3181 gttgtcagca aaatggataa attttattgc ttgtctggcc gattattctc ttgccgattt
     3241 attaaaaatt ctgctacttt cttttttaag atatttggtt ttctcttttc agttttatct
     3301 gatgttgcga ttttttggcg tagaaatcaa cggtttggct gcggcaatag ctattcccac
     3361 tacttacttg tttgtaactt ttactccttc tatggctttt tcagaagctg ccatccgaag
     3421 ctcctattcg gttttggtta tcggcgcctt ctcgggtcaa acggttcaaa ttattctggc
     3481 aagtactttt atatgggtca ttaattttgt aattcccatg tttgtcgggt ctttcgtaat
     3541 gatgcaaacg catccgctga catcgaacaa ataaattttt acgtcatttt ttctcgagtt
     3601 ccaaatctat tcttcccaga taaagtccgc ttttccccat ctgtgccaat acaacaggtt
     3661 taccggctgc attttttaag ttggtttgag taataatttg atgagaatga cctccaatca
     3721 tcacatcaat atacctggtt ttacgtatta tatcgtagtc ggtcagggtt tgcttcagcg
     3781 tgtcggcacc caaatgcgac aggcaaataa tcaaatcgca cttttcgctt ctttttaaaa
     3841 agttggagat attgatagcc gtttccacag gatcgatgta attcattttc ttgtagttat
     3901 tcgtgaaaat cagacctttt ggattgacat tcaaagccat tactccgatt ttcaaaccgg
     3961 ctttgcgaat aatgaggtaa ggttttacca aagttgccat agcagtgctg tccatctgat
     4021 aattggaagt taatatggga aattgagcgt ttttcaacac cgttgccata gtgtccaacc
     4081 cattgtcgaa ttcgtgattc cccaaagtta cagcatcgta tttcattttg ttcatcccat
     4141 ccacctctat tctgccattg taaaaattaa aataaggcgt tccctgccaa aaatcgccgg
     4201 catcgaacaa caacacattt ttattttgtt gccggatttg ttcaattatt cccattcgac
     4261 gagcataacc gcctttatcg gaaacaggaa gatttgattt ttccgtcggc tctatctgac
     4321 tgtgggtgtc gtttgtgtgg agaatggtca actttattct ttctcctcca taaagaatac
     4381 tgttgaacgc caacaaaaaa acaagaatcg cagtattttg gatagttttc ataaaaaata
     4441 gtttttagtt ttccatactt attctgccat caattttgga ttgtataaca tgtcccttca
     4501 aagtttcatt ccgaatgaaa tcgataagga catttcgaag ttttaatccg gtacctgaaa
     4561 ttttttcata ttctgataaa gccgtcattt ggtcattccc ttcggcgaga taatcactgg
     4621 tagctacggt ataaattttt tccgggtcaa tcggatgtgt accgaccaga atttgggtgg
     4681 ctttgccgtt ttttattttc atcgagatac cggaaactcc ctctcctccg acagaagcaa
     4741 aaaattggaa aagttgaatt aacttatctc ctctgaccca aagaattacc aactcatttt
     4801 caaaaggcat taattcgtag atattgcgaa tggtaatatt accggcagga atttcagttc
     4861 gtaatccgcc aagatttacc acagacacat ctacctcttg atgtaaaaaa tccgaagctg
     4921 cacgacgaaa cacatcggca caaaaatttg aaagtggact ttctggtgca aaacttctca
     4981 tattttctgc tgaatagcca attacaacag ccatttcagc atcaattctc tctttcaccg
     5041 gttgaagagc agcctcatat tctttgtcgg caaacttttc agtagtactg tcgataggta
     5101 tttttactga tgttgcttcc actacctgcc acgttttggg gctgcaagag taaaacaaga
     5161 tattaaacag cacaaataaa gtacaaaaat taactttcat cagaatataa tttatttttt
     5221 aaaggtctga tgaaacccac ctgtatgaat attatattca ttcgtagttg tgtacgcaat
     5281 acatgggggt ttcataataa tagattgatt tcaaaatctc ttttctctcg tgtttttgca
     5341 tgcaaattat aaattttatt ccaattttgc tcttaaatat tgttgtggcc attttgagtt
     5401 ttcttttaca tgccgagcac aatgaagagc aaaataggga tttcgcagta attctcttcc
     5461 caaaagcact atatcggctt tttcggttcg tagaatatct tgtatttctt caggcttggt
     5521 aattaatcca actgctgccg taagaatgcc tgttttacgg atttgttccg aaaatgggac
     5581 ctgataacaa ggagctaagg gaatatgcac atttggtaaa attccgcccg acgaacaatc
     5641 aattaagtct actccttcca gataaagaat tttagccaac tcaaccgtat cttccaaacg
     5701 ccatccttga tcagtccaat cggtagctga aagcctcaca aaaaggggca atctttcagg
     5761 ccaaacggtt tgaacggcgg aaacaatttc ctgtaacaaa cgtatgcgtc ctttaaaaga
     5821 accgccgtat tcatctttcc taaaattact gattggcgaa agaaattcat gaataaggta
     5881 cccatgtgca gcatgaattt caagaacatc gaatccagct tgaaacgccc tcagtgctgc
     5941 atctttgaac tgattaacta ccttttttat ttcagacttg gtcaattcat gtggaggaat
     6001 ttcttcagga cgaaaaggaa tagcagaagg agcaatcgtt tgccagccgc cctcttcatt
     6061 ggtgagctgt ttttcacctt tgaaaggaga agaacgagag ccttttcttc cggcatgggc
     6121 aatttgaatc cctgcttttg aatcctgttg atgaataaaa tcaactattt tcttcaatgg
     6181 ttcgatttgt tcatctttcc acaatcccaa atcttcgggc gaaatacggc caacaggact
     6241 aactcccgtt gcttcaaaaa ttattaaagc tattcctcct acacctctcg ttccataatg
     6301 aaccagatgc caatcgtttg ccaatccatc ttgcgcagaa aattggcaca tgggtgacat
     6361 gcccagacga ttttttaaag ttatatcctt tatgcgaaat ggttgaaaaa tttgcaacat
     6421 aacacaaata attatatctt agtttgcttt ttgaatagga aacagtaaaa gattaacaat
     6481 tgttttatac aaaacaacaa aaaaacatcg aattggtaaa aaaattcgta ttttttatat
     6541 atcgtatcag aaaaactttt cgaaatattt agcaaaaatt acaaagacaa aaaacactcc
     6601 ataaaaacta aatttacaaa aaacatttat ctttgcataa actcaatcat tatttcaatg
     6661 cttaaacaac aattacaaca aaaacttcaa caaaaacttt caccggcaca aattcaagta
     6721 ataaaaatgc ttgagattcc tactttggaa cttgaagaac gtattcgtca ggaattggaa
     6781 gaaaatccag ttttggaaat aggcttagaa cccgaagaag aaaaaaagga attggatgac
     6841 tttggtgaag attacgaaga aacaggaaat gacgagggag aaagcaacga tgaaattgat
     6901 tttgaagaat atatgtcgga tgatgacata cccgattaca agttaaaggc aaacaacact
     6961 tcaaaagacg acaaatacga agaaattcct ttctccgccg ggtccacttt tcatgatttc
     7021 ttaatggaac agttgaatct tgttccgctt tcggaaaggg acagggcttt ggcagagttt
     7081 gtcataggca atattgatga agaaggttat ttgcgtagaa cacctgaagc attggcagat
     7141 gatgtagcct ttcaggccaa tatggacact aatgtaaagg aaatagaaaa aatcgttaaa
     7201 attattcaac agtttgatcc tccgggcgta ggagcttcct ctttacagga atgtctgtta
     7261 ctacagttgg aaaagaaaag aaacgagaag gaaattgaaa taccaaaacg cataattcaa
     7321 gactattttg aagaattttc aaaaaagcat tacgataaaa ttataaacgg cctcgaaatt
     7381 gacggtaata cactaaagaa tgccataaac gaaattattc gtctgaatcc gaaacccggc
     7441 aatgcatgga gcggaaacat cttggaaaaa aacatgtcga ttattgtacc tgactttatt
     7501 gtggaaaata acgatggtga actaaccgta catttaaaca acagcaatat tccctatttg
     7561 cgagtcaatg ctgcttattc cgaaatgttt caggattata cttccaacaa ggaaaatcag
     7621 acacctcaga tgaaagaagc tattctgttt gtcaaacaaa aaatcgattc tgcacgttgg
     7681 tttattgatg ccataaaaca gaggcagcag acattattga ctaccatgac tgccattgtc
     7741 gaatttcaaa aagatttttt catcgaagga gatgaaattt atctaaaacc aatgatactt
     7801 aaaaatatag ccgacagaac cggctatgac atttctacta tttccagagt aagcaacaat
     7861 aaatatgtgc aaacagaatt tggagttttt cctctgaaat atttcttttc tgaagccatg
     7921 accaacgatg ccggagaaga aatttcgact cgagaaataa aaaaaattct tcaggaatgc
     7981 gttgataacg aagataaacg acatccatta aatgatgaaa tgctggctga agtattaaaa
     8041 caaaagggct acttaatagc ccggcgtacg gttgctaaat atcgtgagca actaaatatt
     8101 ccggtggctc gattaagaaa agaaatatga atttaaagat ttgtcgtttt taaaaaaata
     8161 taacatttat gaaaatcctc tataaaatta tttcgattat tttacagcct atattgttgc
     8221 caacttatgg catattgctg ctgatacaaa ttcccgtttt tgcaaacctt ccgatgcagt
     8281 ggaaagccat tgctgttgga ggaacatttt tgttcaccgc tttgttgccg tctatacctg
     8341 ttgttataat gtactttagg aaggaagtga gcgatttgtt tatttccaat cgcgaagaaa
     8401 gaaccctacc ttatctgttt tcctttttgg cttatgtgtt ctggacgctt ttcctggtac
     8461 gtaccatgca cttgccaatg tttattggcg gattgggcat tgggtcttcc atatccattc
     8521 ttcttattac ttttatcaat gtcaagtgga aaatcagtgc tcatctggca agtttgggag
     8581 gtttggccgg aggaatattc ggaatttgtt accgatattc cataaatcct ctttggcttt
     8641 ttattcttat actgcttttg gcagcgcttt ctgcaatttc acgtgttgct ctcaaggctc
     8701 acactccaat gcaaaccata gccggttttg ctttgggttt tttatgcgtt tttttacctg
     8761 ttttactgct ttaaaaaaat cttctttgca ggttaactaa aattctatcg caacaataga
     8821 tctgaaagaa cgtcccggca tgggaaaacc tcgaacaatt tcataatttt tgtcgagaaa
     8881 attgtttatt tccagcttga agaagcagga ataagatgat tttttaaaac ttcgtgaaag
     8941 cgacaaatta tgatcggcat aacccggcaa acggttggaa gcataatttt gaaacaaggt
     9001 ataccgatgc cctaaccaga taaatgaata agaaagatta acccaaggca tttccaaagc
     9061 agcttttccg gaaccggaaa ttcttggcgt atacggaatt tgatgcaggt aatcttcggc
     9121 gttaggatca gtaacattca gtgcttgctg gtaagtatac gatcctccaa ataccagtcc
     9181 aatattcctt gttatcgaaa ttccgctttc cattgccaca tccaacccat tgatagagac
     9241 tttaccacaa tttttcatcg tccagttaaa aatatccttg ttaggaaatg caacaatttt
     9301 atctctaatg gcattgtgaa aggcatctaa tgttactgaa agcaatgaaa tactttccga
     9361 tgaaaaagac caagtaatgc cggcattgaa ttgttgcgca ttttcgggct ccaaatttct
     9421 gttgcccacg tatgaataat aaagatcgtt gaaagttggc attctgaaaa catttttata
     9481 aaaactgcgt atcctgaagt catactgttc aaaaggttta acagacaaac tgaaaaaagg
     9541 tgaaaaatgc tttttgtcgt tgggagatga tccaaattta actttttcat tcacaaatgt
     9601 ttgcagacca ccagcagtag ccaaaaattg attggataca tatttggcgt ttaaaaccat
     9661 caatacggtg tttcttttcg gaaaggcaaa ttccggcaag ttggcattca gcgtattcaa
     9721 cgaataatca gttgatgctg aaaaagataa attttcgaaa gccttgtaaa ggaacgaagc
     9781 agaaaagtaa aattcctgtt gtgaatatgt attgtctatt tttcctcccg aattgttata
     9841 atttggatcg acataatgca aataaccatt ttgatatttg gtgttggctt gaaaagacaa
     9901 caaggaagaa aagctacttt cataatgtgc ctgagtaaaa aaagtttgat ctttgcttcg
     9961 ctgatggttg gtattttcgt tcatgtaata aataactgcg cccggcaagc ctctttctga
    10021 ttcataaaaa taggttttca gatagcctga accacgatga aaaaaattgg caaaaattgt
    10081 tccttccaat cgcaaggttt caacatcggt attttttctt gtttctattg aagaactgtc
    10141 ccctgtctgc gtaccataaa tcagcagata aggatactca ccctcagcag taagccattc
    10201 accactaaaa acggcagaaa tttttctgct catttgctgt cgtataaaaa gagaaggatt
    10261 aataagacca aaagaaccta ctttgcaggt aatttttccg tgtatcgatt tttcctcttc
    10321 aaatcgtggt ggtaaggtgc gaatatttaa cacggaagac gaagcaaaag aacgagcggg
    10381 aagaaaaatg ttgtcgcttt gtccattatt taatgagacc atatccacat tgtcaagcga
    10441 aaaacgccca agatcaattt gccccgtctg ataatcggtt agcattatcc catcataact
    10501 cactgctgta tgttcagcac ccagacttcg aactgagaca gttttcaaac cgccaatacc
    10561 tccataatct tttacgttga cacccgcaaa atattttata gcatcagaaa gttgcaatac
    10621 attcagattt tctaattttt ttgccgaaag aatctgcaat ggcatagctg aacgaatttc
    10681 gctgttttga aatttttcaa caactactat ttcgggaatg gaatatgttt ttgccgtatc
    10741 catacccata tcttgaaata atatctctgc tcttacagga ataaaaaaaa acagaaaaag
    10801 aatttcaatc agataaaaga aaaaattttt gtgcataacc ggtttgtaat catttacaaa
    10861 aacagcctca atagaaaaag agaatcaaca taaataaaaa tgtgtagatg gttaaagctc
    10921 tttcctcgaa agctttttaa ctgaataaaa gcatggcagg tcttctggct tgctcttttt
    10981 tttgaacgcc ttcccgtaat gggaaattga aaaaaagata tattattttt ttcgacaacc
    11041 cctgaacagt ggcaacagta ttgttcaaaa aaactacaag agcttacagc agcgggactg
    11101 cagccgattg acacggcttt cccttttcat caattaaccg gatatggttg attgaaacca
    11161 atgcttctgc aaagatacat gaaaatttca aatcgacgca cagacaaata ttagttaatt
    11221 cagatggaaa agagctaaca agtcaaacca tcaacatgca gaaatccgct aaaataaaca
    11281 tacataagaa tttattttac agttaaatac atgaattttt cggtttgtaa aaaatacatt
    11341 tttaagaaaa attgtttgct acatatggtc aactccaatt atttgaatta aaaaacacaa
    11401 aatatgggtt gctttcaggt tgggtttttt tgatacctgt gtcaaacacg actcatccct
    11461 tttcccgtta ttcctctcat cgatccatca gactgtgatt gtccgttttc atcagaatat
    11521 aatttatttt ttaaaggtct gatggaatcc acatgtctga atattatatt cattcgtgtt
    11581 tgtgtatgca atacatgtgg gtttcttaga taaaaaaatc cttttgaact tacggtacta
    11641 aattagttcc aacaaattaa tttttttcat acttttgtat ttattaaaat ttgcattgaa
    11701 attaacatta aatgccttat actaacaaat cataaaccca aaactgatac attccaataa
    11761 agtggaaaag tttggtcttg tcgggcaaag tgtttttgaa tatttttttc atgactaact
    11821 acgaaaaatc ataatactcc aaacgcaagt acatccaatc gattatcatt taattttcaa
    11881 ataaaaacaa gtatcgattt ttttcataca aatttaacca agcttatgaa gattacacca
    11941 tttacgcatt ttcacatcgc tcttggagca aaaatgaccg agtttgcagg tttttacatg
    12001 ccgattgaat atacaggcat caacgaagaa catttgactg tcagacgggc tgccggcgtt
    12061 tttgacgtgt cccacatggg cgaatttctg gtgaaagggc ctcatgcatt ggcttttctt
    12121 caaaaaatga ctaccaacga tgtggctgcc ctcccgttgg gaaaagcgca atattcctgt
    12181 ttcccgaacg ggagaggtgg aattgtggac gatttgctgg tgtatcgcct cgataaggaa
    12241 aattatttgc tggtggtaaa tgcagccaat atcgaaaaag attggaattg gtgcatgcag
    12301 aacaatacgg aaaatgcaga gctggtaaat atttcggaac aggtggctca attggccgtg
    12361 caaggtccca aagctacgca aattcttcag aaactcacgc ctgttcggct ttccgaaatt
    12421 ccttattact catttgctat aggaaagttt gccggtgtgg agccggtgtt gctgtccaat
    12481 accggttata cgggagccgg cggatttgaa ctttatttcc gtcccgattg tgccgatacg
    12541 gtatggaatg ccatttttga agccggagca gacgaaggca ttcgccccat tggactggga
    12601 gcccgagata ctttgcggct cgaaatgggt tattgtcttt acgggaacga tattgacgat
    12661 accacttctc caatagaagc cggtttggga tggattacca aatttgtgga aggaaagaat
    12721 ttcatcgacc gtccattgct cgaaaagcag aaaagagaag gtgtaacgcg aaaactggta
    12781 cgttttgaac ttaccgaacg aggtattccg cgtcatggct acccaattgt aaacaatgaa
    12841 aacaaacccg ttggacaggt tacttcgggc accatgctgc ccgatagcaa aaagggaatc
    12901 gggatgggat atgtggcaac ggaatatgca caacccggta cgaaaattta tattcaaatc
    12961 cgaaacaaga atttggaagc gatagtgcgg aaataaacac attgttaagt acaggaatga
    13021 aaaaggctgc ggttttccgc agccttttca ataattaatc gacagcatgt ttattgctgc
    13081 atgtattctt cgtaggaagt ttcacgatga ccggctaatt ggtaaattgt tcccggatca
    13141 tcttcaaact ctagttccat agttatgctg tccttgttga ttacccgacc attagaaact
    13201 ttgattacaa tgggataacc tgcaatagca ttaattgagc cttctgtttg aaacgtaagt
    13261 gtcttcatat ctacggccgc cttcactttg aatgaccaaa aatgtccatt tgttgcggtg
    13321 gtagcataat cgtgaatcca gatggaatct tgaccaaacg aagtattata agtcataatt
    13381 tcaaatggcc catatatttc tgttccatcc aaataggctt ttaccatcca atcgccacaa
    13441 acaggataag cccaattata ttccttttca tactttggct cgcaggaaaa aagagccaat
    13501 gataaaatag ataggataaa aaatttattt atacgcatag ttatatctcc tttagtttaa
    13561 tggttgtgga ttagttaatg ttaccgtaaa caacattttt ggggtatctt ccattcttgt
    13621 attaagcact atggtttttg tagaaggatc ataagtggat actgtatttt cagttaaaat
    13681 acatggatat ccccaagcta gacgttcagc ggatacaatt tcaaaagtat tatcactatt
    13741 caacttaatg atgcctgggc ctgcataagc cggaccatat cctgaaccat aattatacca
    13801 cccgcctaat agatcttcta tccaatattg atcattacct actccaaaaa caaggatagt
    13861 aaatggacca cgattagcta tagttccatt attatttcgc cttatacgag actgataata
    13921 cccatttaaa ggggcacttg gctcatatac atacactgtt cgcgatgttt gagcgtaaaa
    13981 gccatcagaa tttttaactt tataggtaat ggaatattta cctccttttg ttgcatttac
    14041 atttgaaata acctctatat ttcctgaaat gtcagttgtt ccttcaaaag ctttacatcc
    14101 cggatctaca aaggtggaat ttaaaggcca aaacaattct tcaggcccat tcaattccat
    14161 agtagcataa gttgtaactc ttgaaacacc aagcgactct ttttcacagg aagtaaaaac
    14221 aaaagccaca aatgccaaag tcattaatat tatttttttc ataacgaatt aattttaatt
    14281 gttaattgtt ttacttagcc caccatacag gttctgtaag atccactctt gcaggagtat
    14341 aggggttcga attgacttct ctgctgggga agtacaagcg tttaggtctt tcatctccat
    14401 tcaaaacaga agtaagtgaa cgagtgaaga aattgggata tcccgtacga ttgtaatcga
    14461 aaaatgcttc tattccgttt ttattggctt gtgcgatcca tttttgctca attatcgacc
    14521 gaattccatt atagggatag gttgcatcta tgtcgatggc gtctgctgct aaaccgagac
    14581 ttgtcaagga agcccgtatg gcagttttat aggtgctctc agcatcagaa gccgtcccat
    14641 aacgagcttg agcttcagca atcaaaaaga gtacttcttc ctttgagaaa aaataaacgg
    14701 gacttgtacc cttaatagct ggagtcgcat aatttttact ggtttcagtg gggtgatttt
    14761 tattatcacc tgcagcaata ccattatata agctaccggt aaccgaagga ttaaaaattt
    14821 ttgccattcg tggatcgttc ttatctttta gcaagtcaat aaaagtttta ttggctacaa
    14881 cattacctga caatcgatca acaaaagtat tgtagaaagg attatatccg gtttgttcct
    14941 ctttaaaggc agtaaacttg gcatcttttg tcaaaaactt attttcggcc aacaaagctt
    15001 caatctctgt tctatatgtg tttggattaa cattcacata tcgtaaatac atttttaatt
    15061 tcaatgtatt ggcaaatgct ttccaatcat ctatatttcc gccaaaaata agatcagaag
    15121 agccgggatt ttcaactgtg cttccggtaa aatctttaga cattgcataa tcaatccgtg
    15181 caagtaggct atcataaacc agctggccat tatcataatg aggtataaca ttccccttta
    15241 aagcctctct aaatggaatt tgatcataca aatcagccaa tacctgaaaa gtatatgcct
    15301 gcattaaggt agcgatggca tagaatttcc agttcccagt ctcggcagta tttttacgaa
    15361 catattcgta atcatttaat ccgcctgcat ataaaagtat aaattgacga tcaaagtcca
    15421 catttgtcaa attgtaggat tcccaatcag cccattgaga cgctgcagga gcctgagcat
    15481 attgttgtgt ccagaaactt cccaaagcat gataataacc acccattaca taggccgaat
    15541 tagccaaagc acttggaagt actaatcccg gagttgcttc ggatggattg tttgggtcat
    15601 gatttatgtc aaaaaagctt tcgctgcaac tgcttaagaa tagcaagcca ataaaagcag
    15661 ctatattata aaattttaat cgtttcatat ataattcgtt tttatgtttt ttagaaactc
    15721 aatttaatat taaatcccaa acttcttgtt gatggagttg ccccgaattc accataatcg
    15781 gcagccaaat cgttcccaaa agtagttaat tcaggatccg tgtacaaatt tgagtctgga
    15841 gtccacaaga acaggttatt tcccaccaaa gataaatcaa ctgaattgat aaaagcattc
    15901 ttcaaaaccg atttcgggaa agaataggaa atgctgagtt ctctcagttt aaagaatgtt
    15961 ttgtcaatta acgcataagc atccccaatt cctgccttgt aagtttgatt gtaatacaaa
    16021 ttcaagttgt tagtaaaacc tgcgatagga gtggtgtttt caatatattg tcctccaact
    16081 aactgtacag aattcggaat aataaatggt tgtctgtcgt tgtaggtagt aggttgtgca
    16141 ttaccagtaa agtaagcaat ttctgctgtg cgggaataca tttttcctcc atttctataa
    16201 tcaaatgcag catgtaccct gaaattttta attttcaaag aagttccgcc acccagtcgg
    16261 tatttatttt gagaagaccc aataatttcc tgttcggctt tgaaaacagg taatccttgt
    16321 gcatttacaa caatacgtcc ttgtggatcg gtctcaggaa cattgccttt aaaaagtccc
    16381 aattcatagc ccggacgagc cacaaacggg aagcctcctg ttcctcccaa cacaatttca
    16441 tccaaaccag aaattaaaga tttaagtaaa ttgttgtttt tggaataatt ggcaaaaatc
    16501 tcccattcta tattttttct tcgtataggt gttacactta aactcagttc aactccctta
    16561 ttggtaattt ctcccaggtt ttgagtttgt gaagtataac cggtagaaga tggaagtgtt
    16621 gcagtccaaa ttaaagcagt gatttttttg ttgtaccatg tgaaatctat atccaataaa
    16681 tttttgaaaa gtttcaaatt ggtacctatt tcaaagtctt gagatatttc gggttgtaat
    16741 tggtcgttac caataacgtt ggaaatggta aaaccattca caggaccagc caaaggaaaa
    16801 tccaaggaac gatagccatc cgtaatactg gtttgaagca gtacaggata aattaaatat
    16861 gggtcagcat ctttaccggt acgagcaaag ctggctctaa gttttccata agaaagtaca
    16921 tctttgtttt gaatcaattc agaaaacaaa aagcttccgc ttatgcttgg ataaaagaat
    16981 gaacgattat ttgctggtaa agtagatgac cagtcatttc taccggttat gttcaaaaac
    17041 aacatatcct taaagctcac atccaaattg ccataaacgc caattaatct acgttgagac
    17101 aaagttccat aaatagaagg agtagcagaa ctgtttgaca aattatagaa tttgggaata
    17161 tcgagtccaa ttacttcaga accttgagat tgagcatcgc gctgattaaa attatggccc
    17221 acaatgacac taatcgcaaa atcattattt aattttggat tgaaatttaa aaagaaatcg
    17281 gtattcaatt cggaggagta ataagcattt tctcctactc tacccacttc atcgttgtaa
    17341 tcagctcttg cactgttggt tatggctctc catgttttca atgtgctgtt ggccacatca
    17401 tcgcccaatc tgaaggtggc actcaatgat ggggttatct gtgcatcgag gctgatattt
    17461 ccaaaaatgc ggttttcctg aaatttattg ccatgttcat tcaatatata gtaaggattt
    17521 tgggcataga ttgtgtaata attatctaca ttgttgaatt tgttgttgta atcttttaaa
    17581 tcaacaatac tgatatcgcg tggcgattgc caaagtccat ccaatgcagc ctgatcctgt
    17641 ccggtttctg tgaatctcat atcctttcga acataattca acgaaccgga aatgttaaaa
    17701 attttcagga actttgctga accgcgcatg gaaactgtat tacgattata ataatcagca
    17761 tcggttggca taattccatc agctgttaca ttcccgtaag aaatataata gcttttatca
    17821 gcatcgccat ttgaaacaga aaccgtatta ttataattgg taccgatgtc gaagaaatct
    17881 tttatattgg ttggtaaagc cacataaggt tttatttgtt gttggttgtc aactacaaag
    17941 ccccatgggc gaatcttgcc atcaaagcgg ggtccccagc ttccattttc catcaaatct
    18001 tgttctccgc cacctctatc gtaccaaccc tgaccaaatt cgttctgaaa atagggcaaa
    18061 cgaagtggag agtcgaaagt agaagttgaa gaaacttcaa ctttaggttt attttttaat
    18121 ccttcacctt ttttggtagt tatcaaaata acacccgaag ctgcacgaga tccatacaat
    18181 gcagtagcag aaccgccttt aagtatagtc atcgattcaa tatcttccgg attaatatca
    18241 ttggcacggt taccaaaatc caatcctcca tttatgcttg tcgatccaat ttgtccattg
    18301 ctgatgggaa ctccatcaac aacatacaaa ggttgattgc ttcctcccaa tgaagagtaa
    18361 ccacgcatga taacgcgagt agaagatccg ggagctccag aagcggaagt aatttcgacg
    18421 cccgctacct taccttgcaa tgcatttaat gcacttctgt ttccggtcat ggtaatgtct
    18481 tgagagctca ctgccgaagc cgaatatccc aaagcttttt ctgagcgttt gatgccgatt
    18541 gccgtaacca caacttcgtc aatttccaac gtttgaggtt gcagccttac taccatcccg
    18601 ttttcagctg gaacttccac tgatttcatt ccaatgtagg aaacaaccag tgttcggttg
    18661 ccaaccggca cagtaatgga aaaacttcca tcagtcccgg taattgtgcc agtagtggta
    18721 ccttttacca gtacagaggc gccaataaca ggttgcccat cttcatccga aatcaccttt
    18781 ccttgtacag tcagagattg ggcgttgacc aaaccaactc ctatcataaa taggcaggca
    18841 aataagaatg ttagttttct cataaatttt acatttttgt ttattaatct tattatttat
    18901 tattttcacc tatagcaggg aaaacgtgct ggcaaaaata aaatattaaa aaataaaaaa
    18961 caagtgtttt tgatgacaaa tagaaaaaat attcaatttt tccctttcat tttgcagttt
    19021 attgccaaaa ttcgtatccc tttttaagta ccatcgataa ttttacaaat aaaaatatct
    19081 ttacaaatgt ctgggaataa atgttttact gatttcttaa aattttctgt taaaaaaata
    19141 ttgccataaa taaacaaatt tcattcaaca aaaaattttt tgttaaaatc aaatatttaa
    19201 cattttactc agcaaaaaaa tgacagttga tacaaaaatc actacatttt atgttaattt
    19261 ttaatcaata aaattgaaag tatatctaac agttggctcg aaaacgttag aaaataaatt
    19321 ttataacact tctttatgtt tttttcctta ggacataaca aaaaaattgt aaaaacgttt
    19381 atatttttta ttttcagata tttaataacg gggctttcac caaacctcac ttactttttt
    19441 gtgaggtcat ttaaaaacag accattgctt aaatcgtaat ttttacaaag gaattacagg
    19501 cgataaaatg taatgcttcc agcagctgca tgtatcttca aaagaatgat gaataaatgg
    19561 gaattatcta cttggctcga atttaaaata caaattgttg acttgataaa ccctatgttt
    19621 taccaagcca acaatatttt aagacaaaaa tggggtcttt aaggatcgac gttttaacaa
    19681 aaaacttctt ttatgaatat ccaatagatg ttcaaagggt ttttcgttgt tgtagtaggt
    19741 ttctctgaag ttactcagaa atggttgttg gagaatttga tacgcaaatc taagcaaaac
    19801 ccatttgttt ataaaagctg ctaagatttg gattctttct gattagattc tttaaaccat
    19861 acagtaaaat cgaaacggat ttctaatgtg aaatttaata ggttgttcta aatttatcaa
    19921 tctgaatttt ttgttttact cttgtatcgg ccttgaattc gaaatgcaac attccatttt
    19981 ttttatcaaa agtgaaattt gttaatttct tattgttgag ttttatactt ttacattcag
    20041 cggagacggg aatttctata atccatgtac gttctgtaat cttattttca aattctccct
    20101 gcattgcgtc aatattaatt gtaagttgat gattgcacaa ccaactggtt atgtttgtaa
    20161 ttccaaattc tcctttttga taatttttgg ttttgttgtc atcttcgtat atttcaaact
    20221 tagctgtttt atcaggataa atacgtaaag taagtgtgtc atcgggttct tcagcgcgca
    20281 ttttattttt agctaatgga attatagaac cggctttaac aaaaaccgga ataatgtcaa
    20341 gtggagcgtt ataatcgata gtttgtcctc ccctataagt tttattactc cagaaatcaa
    20401 tccatgtttg gttttctgaa ggcaagtaaa cctgttgatt ggttttacct tcgtcaagta
    20461 caggagctac aagcatttct ttgccaaaca tatattgata atcctgttta cgagctaagg
    20521 agtctgaaaa ttcaaaaaat aacggacgga ttagagtttc gccttttttg taggtcttgt
    20581 atgaatacga atacatatac ggtattaacc gatatctcag gtgtaaatat tttgttgtta
    20641 tactttcggt cagactgtcg aatgcaaaaa cttcacaaaa ctgttcttca ccactgtgtg
    20701 gacgtaacat tggcgaaaat acaccatgtt gaaaccaacg tatgtacaat tcaggttcgg
    20761 ttttcattcg tccatcaaca atatttgtaa aaatggtatt gccatcacgc ctgtcgtggg
    20821 caaaggtaaa tccacctata tcgctggaaa aataaggtat ccctgaaagc acagtacctg
    20881 tcatcatggg tatttgtaac ttaagagcaa accaggtttt agatgcatcg ccactccagc
    20941 acaatgctcc ataacgttga atgccgccaa accctgccct gcttaaattt gtgattcttt
    21001 tgtttggaaa atcagttgaa aaaccatcat aaatgttttt tgcccacaat aaactataaa
    21061 tgttatgtac ttttctccag tcgcccaaat aaaattgtcc gtcaacaaca ggactttcgg
    21121 gttcaacgag gtcgctccaa agtccatcgg caccgaattc aacttccgct ttcagttgtt
    21181 gccaaagcca tttttgggca tcggggtgag ttatatcaaa aagtgaaaca tcgcctgccc
    21241 aaaacggact aaaaacataa ggttcggatt gatttttttt tgaaacgagc catccttttt
    21301 ccttgagaaa tttataatta tatgaatcga tgttgatata tggctcattt attaaaatcg
    21361 tcttgacatt ttttccacgt aatgtattta tatatttgat aggatcggga aaattttgta
    21421 ggttccacga aaaattaccc ataaaatgtg gcgaagttgg tttgagcccg ccaaaccatg
    21481 gtatatcaag tacaatagca tcgacaggca gttttctctg ctcaaatttc tgaagcgtat
    21541 tataaaccat tgtgtcattt tcgtacgaac atttgctctg aatcaaacca taaatccatt
    21601 taggctgcaa gggtggatat ccggtgagat caaaataact ttttagcagt gattgttcat
    21661 taggtgcggc taccagatag tatgtgaaag aaccgttttc gacctcataa taccattctg
    21721 tcggattagt tttgcctaaa tcaaatctgg ctggatactg attgtcaaaa taaactccaa
    21781 atccggttgt agaatatata tatggaatat ttatctgcat ggtaccataa ggcttagtat
    21841 agccaccaat atgctgatta aaaacttcga aagcctcgcc acgacgatcg acggcgatgc
    21901 tcttctgtcc cattccataa aaatgatctg tttcccgtat tttaaacgaa gcctttttgg
    21961 aactaccatc acattcaaaa gcaccagctg actggagcaa tatagacccg tcaggcttgt
    22021 aaaagctaat attaaatggt attttggtaa ttttcgcaac aatcagtctg cctttgataa
    22081 taagttcatt gtgtttctct ttcacagaaa atttgacttc gggcgcctga tttaaaataa
    22141 ctgaatgcga tgaatctatc tctatattat gcatgtcggg aaaccacgaa atgcgaaata
    22201 tattatcgca ccaggcttcc actttgataa ttgagttatc attttggact acgactgtat
    22261 tttttgaaca ggagtatgaa aacaagctgt cgttgaactg tgaataacct gtgtgtaaaa
    22321 ggattgataa aaaaaagcta aaaaaaaatc ttttcatttg gattaaattt attcttgtct
    22381 gacggatgaa tcagttgggt ctacaaatat ataactttaa attttcagag aataatacta
    22441 ttctaacaat ttatgtacat tttctggccc tgaagatatt tatgtaaaat tgaatcccct
    22501 cataaaactt aatttttcta aaggcagaaa aaatcaagtt ttatgagggg ctgaagtaat
    22561 gtataatcaa gtaggtaggg gaatcgcccc tctctcacat caatgtgcat gctctcgcac
    22621 acggcggttt cttttaactg tttaacctct cgtaatttaa tgtgagattt tacaaacctc
    22681 ccatcaaatc gtgaattaag gctcgttcct gttcgcctgc ttcttttact tgcaacgcct
    22741 ttatcgcctg agctccgctt acg
//
LOCUS       DAOSIB010000029        21766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_2664_length_21766_cov_13.202524, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000029 DAOSIB010000000
VERSION     DAOSIB010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21766
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2664_length_21766_cov_13.202524"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..2903)
                     /gene="uvrA"
                     /locus_tag="PLD44_04195"
     CDS             complement(93..2903)
                     /gene="uvrA"
                     /locus_tag="PLD44_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOF98568.1"
                     /translation="MSKQGNIIVKGARIHNLKNIDVEIPRNKLVVVTGLSGSGKSSLA
                     FDTIYAEGQRRYVESLSAYARQFLGRMSKPEVDYIKGIPPAIAIEQKVNTRNPRSTVG
                     TSTEIYEYMKLLYARIGKTYSPISGELVKIHEAEDVVEAAMSYPDDTKLTLLIPIRLN
                     EENDITEKLRNFQMQGFSRVEAGDEMLRIQDLLLRPELLGKNTEIYLVIDRLVVDHSP
                     STISRLTDSVETAFYEGNGNCILKVNTEEGEKLLHFSKNFEADGITFEIPTEHTFSFN
                     SPIGACPVCEGFGKIIGIDEDLVVPNKSLSVYEDAIVCWRGEVMSEWKKRLIQAAPKF
                     GFPIHKPYYELTESQRQLLWTGNEYFEGLNSFFKFLEENQYKIQYRVMLARYRGKTTC
                     PACKGTRLKKEATYVRVGGKPITELVLMPVEELYDFFEQLSLDEHDAAIASRLLTEIQ
                     NRIRFLLDVGLGYLTLNRLSNTLSGGESQRINLATSLGSSLVGSLYILDEPSIGLHPR
                     DTEKLINVLHQLRNLGNTVIVVEHDEEIIRSADYLIDIGPGAGRLGGNIVFNGQLNDI
                     DKQENTKETIANSYTLKYLYHAEEIPVPTLRRKWNNYIEIVGATENNLKNISVKFPLN
                     VMTVVTGVSGSGKSSLVQDVFYAALKKYYGGVVDHTGQFGVLQGSLQLVSQVEFVDQN
                     PIGRSSRSNPVSYIKVYDDIRKLFSEQPLAKQMGYNPSFFSFNTEGGRCEECQGEGTI
                     TIEMQFMADLVLECEHCHGKRFKQDILEVTYRGMNIYDVLEMTVNQAIEFFGQSNNTL
                     EKKIVKRLKSLQDVGIGYVKLGQSSNTLSGGESQRVKLASFLANEKSDPTLFIFDEPT
                     TGLHFHDIKTLLKAFDALIAKGHTVLIIEHNPEVIKCADHIIDLGPEGGDKGGYVVFE
                     GTPEEIVNCGNSYTGKFLKGKI"
     gene            3255..4397
                     /gene="dnaN"
                     /locus_tag="PLD44_04200"
     CDS             3255..4397
                     /gene="dnaN"
                     /locus_tag="PLD44_04200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOF98569.1"
                     /translation="MKFIVSSTELLSHLQTISRVINTKNTLPILDDFKFSLEGNTLTM
                     TASDVETTLITSIQVEESEGEGQVAIASRLLLDTLREFSEQPLTFSINEFNLSLTITT
                     SNGAYNFIGENADEFPRLPPFEEEPCSFELPVDNLLSGISKTLFAAAADDLREVMNGI
                     YFDLSPKELTIVATDAHKLSRYKTTNVSTSLNEDEVKSFVLPKKPANLLKNILPREKG
                     NVKIRFDKKYIHFELENYTMVCLQVAGKYPNYNSVIPFNNPIKIIVDRLMLLNSVRRV
                     SVFCNQATNLIKLVFNEQQVNISAEDIDFSISAEETIPCQLEGNPITIGFKATYLIDI
                     LSNIDSSDVVIELSDPSRAALISPLENEENEEVLMLLMPMLLMDYD"
     gene            4504..5274
                     /locus_tag="PLD44_04205"
     CDS             4504..5274
                     /locus_tag="PLD44_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOF98570.1"
                     /translation="MELNLKKPIVFFDLETTGINIVTDRIVEISYHKVFPNGKELTKT
                     KRINPGIPIPAYATAVHGITDVDVANCPTFKEVAKEIAADFEGSDLAGYNSNRFDIPL
                     LAEEFLRAEVDIDLMKHRFIDVQVIFHKMEQRTLSAAYKFYCNKDLNDAHSAEADAMA
                     TYEVLKAQLEHYPELKNDVDFLSDFTTQNNNVDFAGRVIYNEKGEEIINFGKYKGKKV
                     EDVLKTDMGYYGWIMNSDFSLHTKKVFTNIKLRQFNNK"
     gene            5523..6347
                     /locus_tag="PLD44_04210"
     CDS             5523..6347
                     /locus_tag="PLD44_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOF98571.1"
                     /translation="MNKIVSKEYLSPNVVKFEVEAPLIAKARKAGHFVIVRVGEKGER
                     IPLTIADANTEKGTITLIVQKIGLSTSKLCELNEGDCITDLVGPLGKATHIDNFGTVV
                     CACGGVGTAPMLPIAEAMKKAGNHVITVLAARTKELIILEEQMRKFSDEVIIMTDDGS
                     YGNKGLVTDGVEMVIKRQKVDLCVTIGPAIMMKFVSELTKRYDIPTIASLNTIMVDGT
                     GMCGACRISVGGKTKFVCVDGPEFDAHQVDFDEMLIRLNGYKDIEREQLHKISPNN"
     gene            6415..7881
                     /gene="gltA"
                     /locus_tag="PLD44_04215"
     CDS             6415..7881
                     /gene="gltA"
                     /locus_tag="PLD44_04215"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOF98572.1"
                     /translation="MTNFNEERMQPWRVELRNSMKPKDRMEIARVKMPELDPEYRSHT
                     MKEEVNLGLTPEMALMESKRCIDCANPTCVQGCPVAINIPKFIKNIERGEFLEAAKTL
                     KETSVLPAVCGRVCPQEKQCEAQCIHLKTKNEAVAIGHLERFAADYERETGNISIPEI
                     AAPLHKKVAAVGSGPAGLAFAGDMAKMGYEVVLFEALHEIGGVLRYGIPEFRLPNKIV
                     DAEIRNLIAMGVHFVTNCIIGKTITIDDLLAEDYKGIFIGSGAGLPRFMNIKGENLVG
                     IMSSNEYLTRVNLMDAANPESDTPVIKGKKVAVIGGGNTAMDAVRTARRLGAEKAMIV
                     YRRTEQEMPARVEEIKHAKEEGIEFMTLRNPIEYYGDKNGRVTHMLLQVMELGEPDAS
                     GRRSPVEVKGKTELVEVDEVIVSVGVSPNPLIPQSVKGLEVTGRGTLVVNEETMQTSI
                     PYIFAGGDIVRGGATVILAMGDGRKAAKGMNEWIRSFT"
     gene            7974..8162
                     /locus_tag="PLD44_04220"
     CDS             7974..8162
                     /locus_tag="PLD44_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HOF98573.1"
                     /translation="MENEVSVKVAKGGPYIVSGNFKLVLPSGEEKEMSGKTALCRCGQ
                     SKNKPFCDGSHHDTEFDK"
     gene            8323..9129
                     /locus_tag="PLD44_04225"
     CDS             8323..9129
                     /locus_tag="PLD44_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF98574.1"
                     /translation="MDKLRFQSFGSGSSGNCYYIGNAEYGFLIDAGMGSRTIQNCLKN
                     IGLNFENIQGVFITHDHVDHIRSLWALERKFHLPVFSTRMISEAINQYYYREKNINLP
                     KRRYIEKNETLYIRDFSFTAFDISHDATDNVGYSIEYKGKRITFATDLGCIGKTAASY
                     LILSDYLVIEANYDEQMLRNGKYPDYLKKRISSSRGHLSNDQTGYFLSENYTEKMKNI
                     FLCHLSKENNSPQIALKTIRDYMENKNISVGKDVDVVALNRLNASELYEF"
     gene            9155..9904
                     /locus_tag="PLD44_04230"
     CDS             9155..9904
                     /locus_tag="PLD44_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOF98575.1"
                     /translation="MGENLVIIPTYNEKENIENIIYAVFRQPHDFHLLIVDDASPDGT
                     AEIVKNLQQKFPDRLFMIQRPGKMGLGTAYITGFKWALQHDYQYIYEMDADFSHNPDD
                     LPRLYASCALDGADMAIGSRYLTGVNVLNWPLKRVLMSYFASYYVRIVTGLKVTDTTA
                     GFVCYKRKVLETIDLDKIRFKGYGFQIEMKFTAEKCGFQLKEIPIVFINREYGTSKMS
                     SGIFGEAFFGVIQLKIESWFRDFPKKAIKSS"
     gene            9915..11270
                     /locus_tag="PLD44_04235"
     CDS             9915..11270
                     /locus_tag="PLD44_04235"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOF98576.1"
                     /translation="MSELQLIRNAQIVNEGKIFIGSVLINGELIESVHEGKNNYGEID
                     PNKVEIIEGDNLLLLPGVIDDQVHFRDPGLTHKGDIYSESKAAVAGGVTSFMDMPNTR
                     PPAVNNELLEEKFQLAAEKSLANFSFYIGATNDNLDELLKINPQQVCGIKVFMGSSTG
                     NMLVDDEKTLNAIFRDAPCLIATHCEDELIIRRNKEYYQSLYGENLPAHFHSLIRNED
                     ACFASSAKAVSLAKKYNSRLHILHLSTEKEMSLFDNSVSLEDKKITAEVCVHYLWFSN
                     KDYEQLGNKIKCNPSIKSETDKLSLIRAINNNTIDVIATDHAPHLLPEKEGNCLNAAS
                     GLPLVQHSLVAMLQLAKQGYFTIEKVIEKMCHAPARLFKIDKRGFIRPGYYADITLVH
                     PNDTWQVSKQNIFYKCGWSPFEEMSFDFRVLKTWVNGNKVFDEGKFDEQVRGKRLFFR
                     V"
     gene            11344..12471
                     /locus_tag="PLD44_04240"
     CDS             11344..12471
                     /locus_tag="PLD44_04240"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOF98577.1"
                     /translation="MEFRDLKKQYKVLKNDIDQAMLEVSASGAFIMGKPVKEIEQQLA
                     EYVGVKHCVSCANGTDALTLALKTWGIKAGDAVFVPDFTFFASAEVVSLEGATPVFVD
                     VDADTFNISPASLERSIEKTIQQGKLRPKVIITVDLFGLPADYSAIRKIADKYHLLIL
                     EDGAQGFGGEINGKKACSFGDISTTSFFPAKPLGCYGDGGAVFTDNDEWASLMDSLRV
                     HGKGSFKYDNVRIGMNSRLDTLQAAILKVKFDAFKRYELNDINKVADFYTEKLKDLVK
                     TPFIPKGYLSSWAQYTIQLQDEKQRDGLQAYLKEQGIPTMVYYPKPMHEQTAYLDLGN
                     LSGDFSVTESLCRRVLSLPMHPYLSMEEINKVCQTILDYLK"
     gene            complement(12517..>13269)
                     /locus_tag="PLD44_04245"
     CDS             complement(12517..>13269)
                     /locus_tag="PLD44_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98578.1"
                     /translation="GLTGELKGLGVSPQDYVQENQEIEFSVEELNKVQTEMEILKDKI
                     SELENGIDKLKSEICTCLNIDFTTGWNEVVENLYEREKEILSNLKKNEAYIVAGNVLY
                     KTIEQLQQQEGEELAAGINSGIFTNLLYRLTGKYNKLEIVDNEIIISSENNSWLLKEL
                     STGTKEQVMLALRVTLAQRALNETAFLMLDDAFQHSDYERRPKIIDQLFELSTAGWQI
                     IYLTMDEHIRHLFNQTAQHKQFLENYQEICLS"
     assembly_gap    13270..13369
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13370..14730)
                     /locus_tag="PLD44_04250"
     CDS             complement(<13370..14730)
                     /locus_tag="PLD44_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98579.1"
                     /translation="MGIRIEKITAENLGPLQRFNCEMKNVNLIYGKNEDGKSFLTEFI
                     IRSLFSGTRQWGELREAGNGRIIVSGFNGQEKTFSPRTKKNEKLDVLFSDENGLSPAL
                     AKLLIVKSGEINIAENNLNGIDLNTLKELLSSQKTLNTLAAKISPTIKNSKIDGNLIT
                     INRQGEGKIYYDELSNLNKIRHFIELVSSQSETGKIQELKEEFRLLKKKNEKLLLARR
                     YKAWQYATEIENVHHQLENISESELSELETKIKNCRELQSQKNQLSIEIKTLEERTKD
                     FDETKNNFDRQIAAKQYAAYALNKNISELEKQLGTVEEDEISELEKNLKLLHLYQSEK
                     SEKEHKVEQLKRETEHFDWLEKVRTAYQQLQFSSSEKDTLNKILMVLSVVFAAGGLVT
                     LLALKNILFGAILFAGSFIALSFYVFRLQKTFKNKLRNSEWNQIKQEFQEKFHQELNQ
                     VNL"
     gene            complement(14745..15803)
                     /locus_tag="PLD44_04255"
     CDS             complement(14745..15803)
                     /locus_tag="PLD44_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOF98580.1"
                     /translation="MKIAITADVHLRTKAETPERYLALETLVNSMHDFDASTLVVAGD
                     LFDKDFHNYADFDAFCKLHSSKHFLVIPGNHDLLLEQKYFTASNLEIVSSPEIKILGS
                     IPFVFLPYDAKLSMDEALSNFFIQRKGEEELKNWVLIGHGDYVSRSREMNPYEKGIYM
                     PLSSGSVSAFRPSKVVLGHIHKPYTSGSVWYAGSPFPLDVNETGKRRWLLFDTDDNNL
                     VSVDLPASVIYFVENIVVLPSDNEISYLTQQLEQMIDNWQLLPEDFSKVKLRLSVKGF
                     TTDKAKISGFVNDFLHRKGIGFYDETGIDTTELNILKDEDRISLFEKVKKIIDELPNF
                     DYAGKDDILEQTLKLIFV"
     gene            16088..17458
                     /gene="radA"
                     /locus_tag="PLD44_04260"
     CDS             16088..17458
                     /gene="radA"
                     /locus_tag="PLD44_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOF98581.1"
                     /translation="MAKTKTMYVCQNCGADSPKWIGRCPSCGEWNTYVEEVVSKGNAE
                     KIISGGFTVTAQKPVLLSEVEAIEEKRINTFNNELNRVLGGGLVPGSLVLIGGEPGIG
                     KSTLVLQVVLHLKGKRTLYISGEESVKQIKLRAERLKFDDPDCYLLSETSLEQIFVHI
                     QNIQPDLVIVDSIQTVSTEMLDASPGSVSQVRECAAAFLKFCKTTSIPVILIGHINKE
                     GGLAGPKVLEHIVDTVLQFEGDRHYMYRILRSIKNRFGSTSELGIYEMRQDGLREVEN
                     PSEMLFSQDHEGLSGIAIASAIEGIRPFLIETQALVSSAAYGMPQRSSTGFDLRRLNM
                     LLAVLEKRVGFKLNQKDVFLNIAGGLRVNDPAIDLAVISAILSSNVDIAIEKQVCTAG
                     EVGLSGEIRPIHRIEQRILEAEKLGFRKMIVPANNLKGLSLKNIEMEIIPVKKVEEAF
                     KILLKK"
     assembly_gap    17495..17594
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17604..21203)
                     /locus_tag="PLD44_04265"
     CDS             complement(17604..21203)
                     /locus_tag="PLD44_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF98582.1"
                     /translation="MRQNGKISFFGFLLVFVLAGCFCEYFYQTSPQRVVHFNQFQSKL
                     ISKELSTARILKQMSNLLDNHSVNELINFSFPDDDISFYVYRGNELIFWSDHHLDVSS
                     ISLNDSVELHFVQLPNAYCVSKTLSADSLKLLALIKIKNNFPYENDQLINDFAKGFNL
                     NKNVGIVGGSNLDKYAVFSSHGDYLFSLSEPTVPVYDERWALAGLISFLLFFLLFFVW
                     YARGFSLSRTKPLQWKDFCGYVLITGALLAVMLYFNFPSVLLANKLFAPYLYAVPPLL
                     NSTVHLTLLTVYIFSTVFLFYRHVQLGNSVSFLRSFGLLLMLPLYFLLFYYVLRGVVY
                     HSGPQILILSFKNFSIFTVWMHILMLLWGIGFALLFFKIHTFLTRHYYMKIAFAIDAI
                     FSCLLAMAAFKVFPEMWKLSFFSYLFLWIPLYGFYIVNFSRKRNVKLIIYVLFFSIFF
                     IGNVVVLSNEKKFNKYRIAAENLSLNGTIENDQMTEILMEELDAQLSRDRFVNQLMRN
                     SDSVEVLSEYLNNSYLRGFWNRFEVHINAVSKNSDLCKEYQLFLQEKGIKIKQTHFYN
                     IIPNEKNISYLGVFSVLTAGNDSTCYFMEFYPRQNFRSYSFPNLLISSPPSILSQLNV
                     SLARYDHRKLVYRNGNFVYPDVSNWIPFENSNYFVVHYADRRHYVYAPDRNTYLVLTE
                     NVQYGTGAYLLYFCYAFLAFFALCWLLQQVDDFFAKGRKFTLSFINRFQISFIALLIL
                     SFIGIFYSSVDYIRNNYEKEQIQNLENKKIYIQKALQNLYYWNKDLSDKNTQTLNLDL
                     QDLSYTFHTDVHVYNNDGILVGSSQPLIFSKKLISNRIAPKPYFTEDANINQYENIGH
                     LNYLTAYTDFFNGDYLQIGYIAVPQFFSQDEIQNEIESFISVIVHIYFVIILLVIILT
                     LLIGNQISAPLMMLEKKLKEIRLGRRNEKIDYRYDDEIGQLVAQYNRTVDELEESARL
                     LARSERESAWKSMARQVAHEINNPLTPMKLTIQQLSRTKKMDKEKFNEYFDKASQMLV
                     EQIDNLSQIARAFSDFARMPEAKFERVELEELLKSEVEFFKHNYEKVDISFEGSKEPI
                     YTFADPKQLIQVFNNLIKNAIQSIPENRQGKIAVKISTSHQQIEIRISDNGAGIPEDI
                     RDKLFIPNFTTKSTGMGLGLAISKNIIEVTGGNISFTTQIDKGTTFIVTLPKA"
     gene            complement(21247..21720)
                     /gene="rlmH"
                     /locus_tag="PLD44_04270"
     CDS             complement(21247..21720)
                     /gene="rlmH"
                     /locus_tag="PLD44_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOF98583.1"
                     /translation="MKFVLINIGKTLDIHLSSLQEQYQNRLKHYLNFEVINIPELKNT
                     KNLSSSEQAAKESDLILKKLEIGDDVILLDERGTEFTSVEFADFLSKKMHSSNKRIVF
                     VTGGAYGFSEELYRRANNMVSLSRMTFSHQMVRLIFVEQLYRAMTILKGEPYHHA"
BASE COUNT         6814 a         4033 c         4126 g         6593 t
ORIGIN      
        1 caacatctct acttttattt cttatttcgt aattcaaagc tcagaactct ggactcaaaa
       61 ctcaaaacta tatttttttg gtaaaatgaa atctaaattt ttcctttcaa aaactttccg
      121 gtataggaat ttccgcaatt tacaatttct tcgggagttc cttcaaaaac cacataacct
      181 cctttgtcgc cgccttcggg ccctaaatcg ataatgtgat cggcacattt gattacttcg
      241 ggattgtgtt cgataatgag aacggtatgg cctttcgcaa tcagtgcatc aaaagccttg
      301 agtaaagttt tgatgtcatg gaaatgcagt ccggttgtag gttcatcgaa aataaagagt
      361 gttgggtctg atttttcgtt tgccagaaaa gaagccaatt ttacccgctg actttcgccg
      421 cccgataagg tattggatga ttgtcccaat ttgacatatc cgataccgac atcctgcaat
      481 gacttgagac gtttcacaat tttcttttcc aacgtattgt ttgattgtcc aaaaaattcg
      541 atagcctgat tcacagtcat ttccagcaca tcatagatat tcattcctcg ataggtaact
      601 tcgagaatat cctgtttaaa acgtttcccg tgacaatgtt cacattccag taccaaatcg
      661 gccataaatt gcatttcgat tgtgatggtt ccttctccct gacattcttc gcaacgaccg
      721 ccttcggtat tgaaagagaa aaaggaagga ttataaccca tttgtttggc caatggttgt
      781 tcggaaaaaa gttttcttat gtcgtcatac acttttatat aactcaccgg attggagcga
      841 gaagaacgtc caatgggatt ttggtcgaca aattctacct gactgaccag ttgcaaactg
      901 ccttgcaaaa caccaaactg tccggtatga tcaaccactc caccataata ttttttcaaa
      961 gcagcataaa atacatcctg aaccagcgac gattttcccg aaccgctgac accggttacc
     1021 actgtcatta cattcagagg aaattttacg gaaatatttt ttaaattgtt ttccgtagct
     1081 ccgacaattt ctatgtaatt gttccatttg cggcgaaggg taggaacggg gatttcttcc
     1141 gcatgataca aatatttcaa tgtgtaagaa ttggcaatag tttccttcgt attttcctgt
     1201 ttgtcaatat cgttcagttg accgttaaag acaatattcc cgcccaatct gccggctccg
     1261 ggaccaatat cgataaggta atctgccgaa cgaataattt cttcatcatg ttctaccaca
     1321 ataaccgtat ttcccaaatt acgcagctga tgcaatacgt tgatcaactt ttcggtatcg
     1381 cgcggatgaa gtccaatact cggttcgtcc agaatataca gcgaccccac caagctgctt
     1441 cccaaagaag ttgccagatt aattcgctga ctttctccac ccgaaagtgt attggaaagt
     1501 cgattcagtg taagatagcc caatcctaca tcaagcaaaa aacggatacg attttgaatt
     1561 tctgtcaaca aacgactggc gatggctgcg tcgtgttcgt caagcgaaag ttgttcaaaa
     1621 aaatcgtata attcttccac cggcatcaac accaattccg taatgggttt tccaccgacg
     1681 cgcacataag ttgcttcctt tttaagtcga gttcccttgc aagccggaca ggtggttttt
     1741 ccccgatacc ttgccagcat cacgcgatat tgaattttgt actgattttc ttccagaaac
     1801 ttgaaaaagc tgttcaatcc ttcaaaatat tcattccctg tccaaagtag ttgtcgttga
     1861 ctttcagtaa gttcgtaata aggtttatga atggggaaac caaattttgg tgcagcctga
     1921 attaaccttt ttttccattc actcattact tcgcctctcc agcatacaat ggcgtcttcg
     1981 taaacggaaa gcgatttgtt gggaacaaca agatcttcgt caatgccgat aatttttcca
     2041 aagccttcac acaccggaca tgcaccaatg ggactgttga agctgaaagt gtgttcggta
     2101 ggaatttcaa atgtaatgcc gtcggcttca aaattttttg aaaaatgtag cagtttttct
     2161 ccttcttccg tattcacttt cagaatacag tttccattgc cttcgtaaaa agcagtttcc
     2221 accgaatccg tcaaacggct tatggtggat ggggaatgat caaccaccaa tcggtcgata
     2281 accaaatata tttcagtgtt ttttccgagc aattcggggc gaagcagcaa atcctgaatt
     2341 ctgagcattt cgtcgcccgc ttccactcga ctgaatccct gcatttggaa atttcgaagc
     2401 ttttcagtta tatcgttttc ttcgttcaac cgaatgggaa tgagtaaagt aagttttgta
     2461 tcgtcgggat acgacatggc tgcttccacc acatcttctg cctcgtgaat ttttaccaat
     2521 tctcccgaaa ttggcgaata cgtttttcca atgcgcgcat aaagcaattt catgtactcg
     2581 taaatttcgg ttgaagttcc aaccgttgat cgcggattgc gagtgttgac cttttgttcg
     2641 atggcaatag ccggcggtat gccttttatg taatcgactt caggtttgct cattcgtcct
     2701 aaaaattgtc gtgcataagc cgaaagactt tctacgtatc tgcgttgtcc ttcggcatat
     2761 atggtgtcga aagctaaaga tgattttccc gaacccgata agccggtaac taccaccaat
     2821 ttgtttctcg gaatttcaac atcaatgttt ttaagattgt ggatacgtgc gcctttaaca
     2881 ataatatttc cttgtttgga cattcaactg ctccctaaaa attaaaataa acaatagaaa
     2941 atcagattgt tatttaaaaa tttttctgat tattcttcag aaaacgacga tttttaaata
     3001 acctgcaaag atacaattta ttgagcggat gagtaataga tgcagataaa atttattgaa
     3061 tatagttatt agctgagtca tatggaattt tattgtttta attttcgaat gagaaacaaa
     3121 acactgaaat tgtttcatat agccaattga tggagtgaat tattaataaa aaataaaaga
     3181 ttatttttta aaaattaaaa tattctgtac atttgctcaa ttaaacgatt cagtataaaa
     3241 taaaaactaa acgtatgaaa ttcatagttt ccagcacaga acttttgagc catttgcaaa
     3301 cgattagcag ggttatcaac accaagaata ctttgcctat tctggatgat ttcaaatttt
     3361 ctctcgaagg caatactctg acgatgactg cttccgatgt tgaaacaacc ctcatcactt
     3421 ccatacaggt ggaggaatct gaaggagaag gtcaagtagc tattgcttct cgtttgttac
     3481 tggatacact gcgcgagttt tcggaacagc cgctgacttt ttccattaac gaattcaatt
     3541 tgagcctcac catcaccact tccaatggtg cttataattt tattggtgag aatgcggatg
     3601 aatttcctcg tttgcctcca tttgaagaag aaccttgttc ttttgaactc cctgttgaca
     3661 acttgctttc gggaatttcc aaaactttgt ttgcagctgc tgccgatgat ttgcgtgaag
     3721 tcatgaatgg tatttatttt gatttatctc ctaaagaact caccattgtt gcaaccgatg
     3781 cacacaaact ttcgcggtat aaaaccacaa atgtgagtac ttctttgaat gaagatgaag
     3841 taaaaagttt tgtacttccc aaaaaaccgg caaacctttt gaaaaatatc ctgccaagag
     3901 aaaagggtaa cgtaaaaatt cgattcgaca agaagtacat ccattttgaa ctggaaaact
     3961 atacgatggt ttgcctgcag gtggccggga aataccccaa ctataacagt gttatccctt
     4021 tcaacaatcc cattaaaatt attgttgacc ggcttatgct gctcaattcc gtcagaaggg
     4081 tttccgtatt ttgcaaccag gcaaccaacc tgataaaact ggttttcaac gaacagcagg
     4141 taaatatttc ggccgaagac attgattttt ctatctctgc cgaagaaacc attccttgtc
     4201 aattggaagg aaatcctatc actatcggat tcaaagctac ttatcttatc gacattcttt
     4261 ccaatatcga ttcttctgac gttgtaatcg agctttccga tccaagtcgc gctgcattga
     4321 tttcccccct cgaaaacgaa gaaaacgaag aagtcttaat gctccttatg ccgatgcttt
     4381 taatggatta cgattaaatt tacaattttt tcagtcggat tatttttaag gtctaaggtg
     4441 aaaaaacttt agaccttgtt ttttcgattt tagctttgtt caatttttcc aaaaaaaaat
     4501 ttcatggaat taaatttaaa aaaaccgatt gtttttttcg acctcgaaac aacaggtatc
     4561 aacattgtta ccgaccgtat tgtggaaatt tcctatcata aggtttttcc taacggaaaa
     4621 gaacttacga agactaaacg tattaatccc gggatcccta ttcctgccta tgcaactgct
     4681 gttcatggca ttaccgatgt cgatgtggcc aattgtccta cttttaagga agtagctaaa
     4741 gaaatagcgg ccgattttga aggaagcgat ttggcaggtt acaactccaa tcggttcgac
     4801 attccacttt tggcagaaga atttttgcgt gcagaagtag atatcgattt gatgaaacac
     4861 cgatttatag atgtacaggt tatttttcac aaaatggagc agcggacttt gtcggcggct
     4921 tacaaatttt actgcaacaa ggatttgaat gatgcccaca gtgccgaagc cgatgcaatg
     4981 gctacttatg aagtgctgaa agctcaattg gaacattatc ccgaattgaa aaacgatgta
     5041 gattttttat ccgattttac tacccagaac aacaatgtgg actttgccgg acgcgtcatt
     5101 tacaacgaaa aaggagaaga aattatcaat ttcggtaaat acaagggcaa aaaggtggaa
     5161 gatgtactta aaaccgatat gggatactat ggatggatta tgaacagcga tttttcactc
     5221 cataccaaaa aagtttttac caacattaaa cttcggcaat tcaacaacaa atagagatta
     5281 ctttaccgta aatttttttg ccaagtctgc tgtaattttc tttacattca gcaaaatatt
     5341 ccgaaaaatt ctaatttcag gcatttaatg cccatttatg tcaatgacaa ctctatcaaa
     5401 atttttcttt tttaagaaaa aacttgttgt ttcttaaact tttcaataat gcatttaatc
     5461 tttttgtgtg aaaattgaaa attatcgtgt aattttgtct tttgaaacta aaacagccaa
     5521 aaatgaataa aattgtcagc aaagaatatt tgtcccccaa cgtggtaaag tttgaagtgg
     5581 aagctccatt gatagcgaaa gctcgcaaag caggacattt tgtgattgtg cgggttggag
     5641 aaaaaggaga acgcattccc ttaaccattg ccgatgccaa tacagaaaaa gggaccatta
     5701 ctttgattgt tcaaaaaatt ggtttgtcca cttccaaact ttgcgaattg aacgaaggcg
     5761 attgcataac cgatttggta ggtccgctgg gaaaagcgac ccatattgat aattttggaa
     5821 cggtagtttg cgcttgtggt ggtgtaggaa cagctccgat gcttcccata gccgaagcca
     5881 tgaaaaaagc gggtaatcac gtaataacag ttttggccgc acgtacaaag gaattaatca
     5941 ttttggagga acaaatgaga aaattttccg atgaagtaat cattatgacc gatgatggct
     6001 cttatggcaa caaaggtttg gtaaccgatg gtgttgaaat ggtcataaag cgccaaaaag
     6061 tagatttatg tgtaactatc gggccggcaa tcatgatgaa atttgtgagt gaactgacaa
     6121 aaagatatga tattcctact attgcttcgc tcaataccat catggtagat ggaacaggca
     6181 tgtgtggcgc ttgccgcatt tccgttggag gaaaaacaaa attcgtttgt gtagatgggc
     6241 ctgaatttga tgctcatcag gttgattttg atgaaatgct gatacgctta aacggataca
     6301 aggatatcga aagagaacaa ttacacaaaa tatctcctaa caattgattc cccagcaaaa
     6361 tatttttgtt gtaaaaacaa attacttttt tgaaaatttt ttaagtccga agaaatgaca
     6421 aattttaatg aggaaagaat gcaaccttgg cgggtggaac tgagaaattc catgaagccg
     6481 aaagacagaa tggaaattgc cagagtaaaa atgcccgaac tcgatcccga atatcgcagc
     6541 cacaccatga aagaagaagt caatttgggt ttaacgcccg aaatggcttt gatggaatcg
     6601 aagcgatgca ttgattgtgc caacccaact tgtgttcaag gttgccctgt ggctatcaat
     6661 ataccgaaat tcatcaaaaa tattgaacga ggagaatttt tggaagctgc caaaacatta
     6721 aaagaaactt cagtattgcc ggcagtttgc ggacgagttt gccctcagga aaaacagtgt
     6781 gaagcacagt gcattcattt aaaaacaaaa aacgaagcgg ttgccattgg tcatctcgaa
     6841 cgttttgctg ccgattacga gcgagaaaca ggcaacatat ccattcccga aatagccgct
     6901 ccgcttcaca aaaaagttgc tgctgtaggt tcaggccctg ccggcttggc ttttgccggc
     6961 gacatggcaa aaatgggcta cgaagtggtg ctttttgaag cattgcatga aattggtggg
     7021 gttcttcggt atggaatacc ggaatttcgt ttgcccaaca aaattgtaga tgccgaaatt
     7081 aggaatctga ttgctatggg tgtacatttt gtgaccaact gcattattgg caaaaccatt
     7141 actatagacg acttactggc tgaagattat aaaggtattt tcattggaag tggagccgga
     7201 ttgcctcgtt tcatgaatat aaaaggcgaa aatctggttg gcatcatgtc atcaaacgaa
     7261 tacttgacac gtgtcaattt gatggatgca gccaatcctg aatcggatac acctgttata
     7321 aaagggaaaa aggtggccgt aattggtggt ggaaatacgg caatggacgc agtaagaacc
     7381 gccagacgtc ttggcgcaga aaaagccatg atcgtttatc gccgaacaga gcaggaaatg
     7441 cctgcccgtg ttgaagaaat caaacatgcc aaagaagaag gaattgagtt tatgacttta
     7501 cgaaatccca ttgaatacta tggcgataaa aacggaagag ttacccacat gctgcttcag
     7561 gttatggaat tgggcgaacc cgacgcttcg ggacgccgct ctccggtaga agtcaaaggg
     7621 aaaaccgaac tggtggaagt tgatgaagta attgtcagcg taggagtttc acccaatccg
     7681 ctcattccac aatcggtaaa gggactggaa gttaccggca gaggaacatt ggtagtgaac
     7741 gaagaaacca tgcagacaag tattccctat atttttgccg gcggagatat tgttcgtggt
     7801 ggtgctacgg ttattcttgc tatgggtgac ggaagaaagg ctgcaaaagg gatgaatgaa
     7861 tggattagga gctttacttg aaatttgaat aagatttaaa aaatattttt taaaaaactt
     7921 tttcctatta attttgtttc catttacaaa gttaaattta aaatcttact aaaatggaaa
     7981 atgaagtttc agtgaaagtt gccaaaggag gcccttacat cgttagtggt aatttcaagc
     8041 ttgttttacc ttcaggagaa gaaaaagaaa tgagtggtaa aacagcactt tgtagatgtg
     8101 gacaaagcaa aaataaacct ttttgtgatg gttcacatca tgatacagag ttcgataaat
     8161 aattgaagaa ttgttgattt tattaaagga aacagggata aaaaattgtc tcctgtttct
     8221 ttttttcttt ctgaggatgt tgaaacatta actttatttc cgaataattt tttggtgcaa
     8281 tttccgatta atgttaattt tgttctttct aaaatttatt gaatggacaa attacgattt
     8341 caaagttttg gtagtggcag tagtggaaat tgttattaca ttggaaatgc cgaatatggt
     8401 tttttaatag atgcaggcat gggatcgcga actatccaga attgcctgaa gaatataggt
     8461 ctgaatttcg aaaatattca gggcgtgttt atcactcatg accacgtcga tcatatacgt
     8521 tcattgtggg cattggaaag aaaatttcat cttccggttt tttccacccg aatgatttcg
     8581 gaggcaatca atcaatatta ttatcgtgaa aagaatatta atttgcctaa acgaagatat
     8641 atcgagaaaa atgaaacact ttatataagg gatttttcct ttaccgcctt tgacatttct
     8701 catgatgcta cagataatgt tggttacagc attgaatata aagggaaaag aattacattt
     8761 gcaacagatt tgggttgtat tggaaaaaca gccgcatcct atttgatttt gtccgattat
     8821 ttagttatcg aagccaatta tgatgagcaa atgctccgaa atgggaaata tccagattat
     8881 ttaaaaaaaa gaatttcttc ttccagaggg catttgagca acgatcagac cggttatttt
     8941 ctttctgaaa actatacaga gaaaatgaaa aatatatttc tttgtcatct cagcaaggaa
     9001 aacaactctc cacaaattgc tctgaaaacg atcagagatt atatggaaaa taaaaatatt
     9061 tcggtaggca aggatgtgga cgtggtagcg ctgaatcgac taaatgcttc cgaattgtac
     9121 gaattttaaa tttattcctc tgcaaattaa aactatgggt gaaaatttag ttattattcc
     9181 tacttacaac gaaaaggaaa acatagaaaa tattatttat gctgtttttc gtcagcccca
     9241 tgatttccat ttgttgattg ttgatgatgc ttcccccgat ggaacagcag agatagtgaa
     9301 aaacttgcaa caaaaattcc ctgatcgatt gtttatgatt cagcgcccgg gaaaaatggg
     9361 actcggaact gcttatatta ccggctttaa atgggctttg cagcacgatt atcagtatat
     9421 ttacgagatg gatgccgatt tttctcataa tcccgacgat ttaccccgat tatacgcttc
     9481 ttgcgctctc gacggagcag atatggctat tggttcacga tatctcacag gcgtgaatgt
     9541 attaaattgg ccgctaaaga gagttttaat gtcttatttt gcttcctatt atgttcgtat
     9601 cgttaccggt cttaaagtta ccgatactac agcaggattt gtttgttata aaagaaaagt
     9661 gctggaaacc attgatctgg ataaaattcg ttttaaaggt tatgggtttc agatagaaat
     9721 gaaatttacg gctgaaaaat gcggattcca attaaaagaa attccaattg tttttattaa
     9781 tcgggagtac ggcacttcaa aaatgagcag tggtattttc ggagaagctt tttttggtgt
     9841 aatccaattg aaaattgaaa gctggttcag ggatttccct aaaaaagcaa tcaaatccag
     9901 ttaaattatt tattatgagt gaactgcaat tgattagaaa tgcacaaata gttaacgaag
     9961 gaaaaatatt catcggttct gtgctcataa atggagaact aattgagtca gttcacgaag
    10021 gaaagaataa ttatggagag atagacccaa ataaggttga aatcattgag ggagacaatt
    10081 tacttctttt gcccggcgtt atagatgatc aggttcattt tcgcgacccc ggacttactc
    10141 ataaaggtga tatttattct gaaagtaaag cagctgtagc tggaggagtt accagtttta
    10201 tggatatgcc caatacgcgt cctcctgctg taaataatga attgctcgaa gaaaaatttc
    10261 aactggcagc cgaaaaatcg cttgccaatt tttcctttta tattggagct accaatgaca
    10321 atttggatga attactgaaa ataaatcctc aacaggtttg tggtataaaa gtttttatgg
    10381 gttcttctac agggaatatg ttggtagacg acgaaaaaac attaaatgcc attttccgtg
    10441 atgccccctg tctgattgca acacattgtg aagatgaatt aattatccgc agaaacaagg
    10501 aatattatca atccttgtat ggagaaaatt tacccgccca tttccattcg ctcattcgta
    10561 atgaagatgc ttgttttgct tcttcggcga aagcagtttc gttggcaaag aaatataatt
    10621 cgagattgca cattttacat ctttcaaccg aaaaggaaat gtctttgttt gataactctg
    10681 tttcacttga agacaaaaaa ataacggctg aagtttgcgt tcactatttg tggttttcta
    10741 ataaagatta tgaacagttg gggaacaaaa ttaaatgtaa cccttccata aaatcagaaa
    10801 ccgacaaact ttcacttata cgggcgataa acaataatac cattgatgta attgctactg
    10861 atcatgctcc acatttactt cccgaaaagg aaggtaactg tttgaatgca gcttccggat
    10921 tgcctttggt tcagcattca ttggtagcga tgttgcaatt ggcaaaacag ggttatttta
    10981 ccattgaaaa agtaatcgaa aaaatgtgtc atgcgccagc acgattattt aaaatagaca
    11041 aaagaggctt tattcgtccg gggtattacg cagatataac attagtacat cctaacgata
    11101 cttggcaggt ttccaaacaa aatatttttt ataaatgcgg gtggtcgcca tttgaagaaa
    11161 tgagtttcga ctttcgggta ctgaaaacat gggtcaatgg aaataaagta tttgatgaag
    11221 gaaaatttga tgagcaagtg aggggaaaac gattgttttt tcgagtataa acaattcttt
    11281 tcatcagaat ataattagtt aattatagag caaataaaat ttgaaaccac aaataataaa
    11341 actatggaat tcagagattt aaaaaagcaa tacaaagttc tgaagaatga tattgatcag
    11401 gccatgttgg aagtaagtgc ttcgggcgca tttatcatgg gaaaacctgt caaagaaata
    11461 gaacagcaat tggccgaata tgttggcgta aaacactgtg tttcttgtgc aaacggaaca
    11521 gatgccttga cattggcttt aaaaacatgg ggaataaaag ccggagatgc agtttttgta
    11581 cctgatttca ctttttttgc ttcagccgaa gttgtttctc tcgagggagc tacacctgtt
    11641 tttgttgacg tggatgccga tactttcaat atttctcctg catctttgga acgaagcata
    11701 gaaaaaacca ttcaacaggg aaaacttcgg cctaaagtca ttattacggt cgatttgttt
    11761 ggactacccg ccgattattc tgctattaga aaaattgccg acaaatatca tctgttgatt
    11821 ttggaagatg gagcacaagg tttcggagga gaaattaacg gcaaaaaagc ttgcagtttt
    11881 ggcgatattt ccactacttc gtttttcccg gctaaaccct taggttgtta tggtgatggc
    11941 ggagcagttt ttaccgataa cgacgaatgg gcttcgctga tggattcctt gcgggtacat
    12001 ggcaaaggtt cttttaaata tgacaatgtg cgtataggta tgaactcacg tctcgataca
    12061 ttgcaggctg ccattttaaa agtgaaattt gatgctttca aaagatatga attgaacgac
    12121 atcaataaag tagccgattt ttatacggaa aaattaaaag atttagtgaa aacgcctttc
    12181 attcccaaag gttatctttc cagttgggca caatacacca ttcaattgca ggatgaaaag
    12241 cagcgagatg gcttgcaggc ttatttgaag gaacaaggta ttcccacaat ggtgtattat
    12301 ccaaaaccca tgcatgagca aacagcctat cttgatttgg gcaatttatc aggtgatttc
    12361 tccgtaaccg aatccctatg ccgcagagta ctttctctac caatgcatcc ttacttgagt
    12421 atggaagaaa ttaataaagt ctgccaaacg attttagatt atctcaaata aatttcaact
    12481 gttatctctg ataagacttt aataatttta tatcatttac gacaaacaga tttcctgata
    12541 attttcaaga aattgcttgt gttgagctgt ttggttaaaa aggtgacgaa tatgttcatc
    12601 cattgtcaga taaatgattt gccagccggc agttgacaat tcaaacagtt gatctataat
    12661 tttggggcgt cgctcgtaat cgctatgctg aaaagcatcg tccaacatca gaaaagccgt
    12721 ttcgttgagt gctctttgtg ccaaagttac gcgaagagca agcatcacct gttctttggt
    12781 tccggtgctg agttctttca gcagccacga attattttca ctcgatatga ttatttcatt
    12841 atcaacaatt tccagcttat tgtatttccc agtaagccga taaagcaaat tggtaaaaat
    12901 gccggaattg ataccggcag ccaattcttc accttcctgc tgttgaagtt gttcgattgt
    12961 tttgtaaagt acatttccgg ccacaatgta cgcttcgttt tttttaagat tagagagaat
    13021 ttccttttct ctttcgtaaa gattttcaac cacttcgttc catcctgtgg taaaatcaat
    13081 atttaaacac gtgcaaattt ccgatttcaa cttgtcgatt ccattttcga gttcggaaat
    13141 tttatctttt aaaatttcca tttcggtttg aaccttattc agttcttcca ccgaaaattc
    13201 aatttcctga ttttcctgca cataatcttg aggcgaaaca ccgagtccct tcaactctcc
    13261 cgttaggccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ccagatttac
    13381 ctgattcagt tcctgatgaa atttttcctg aaattcttgc ttgatctgat tccattccga
    13441 atttcggagt ttatttttga aagttttttg aagacgaaaa acgtaaaatg acaacgctat
    13501 aaatgaaccg gcaaacagta ttgcgccaaa aagaatgttt ttcaacgcta aaagtgttac
    13561 caaaccgcct gctgcaaaga caacagataa aaccatcaat attttgttga gcgtatcttt
    13621 ttctgacgac gaaaattgaa gttgttgata agccgtgcgg actttttcca accagtcgaa
    13681 atgttccgtt tctcttttca actgttcaac cttgtgctct ttctcagatt tttcggattg
    13741 gtagagatgg agtaatttga gatttttttc cagttctgaa atttcgtctt cctcaacagt
    13801 accgagttgt ttttccaatt ctgaaatatt tttgttcaat gcgtaggcag catattgttt
    13861 tgctgcaatt tgccgatcaa aattgttttt cgtttcatca aaatcttttg tccgttcttc
    13921 gagcgttttt atttcgatac tcaactgatt tttttgggat tgaagttctc tacagttttt
    13981 aatttttgtt tccagttcgg aaagttcgga ttcggaaata ttttccaatt gatgatgaac
    14041 attttcaatt tcagtggcat attgccatgc cttgtagcga cgagccaaca acagtttttc
    14101 gtttttcttc tttaaaagac gaaattcttc tttcagttcc tgaatttttc ccgtttcaga
    14161 ttggctggag accaactcga taaagtgccg gattttattg agattactta gctcgtcgta
    14221 ataaattttg ccttcaccct gtcgattaat ggtaatgaga ttcccgtcaa tttttgaatt
    14281 ttttatggta ggagatatct ttgctgccag tgtattcaat gttttttgtg aagaaagcag
    14341 ttctttcagg gtatttaagt cgataccgtt caaattgttc tcggcgatgt ttatttctcc
    14401 acttttgaca atcaataatt tggccaatgc cggcgaaagc ccgttctcat cagaaaaaag
    14461 cacatccagt ttctcgttct ttttagttcg gggtgaaaaa gttttttcct gtccgttaaa
    14521 acccgaaaca ataattctgc cattgccggc ttcgcgcaat tcgccccact gacgagtgcc
    14581 ggaaaaaagc gaccgaataa taaattcggt cagaaaagat ttgccatctt catttttccc
    14641 ataaatcaag ttaacgttct tcatttcaca attgaaacgt tggagcgggc ctaaattttc
    14701 tgcggttatt ttttcaattc ggattcccat gattatattt gtgattatac aaaaatcaat
    14761 tttaaagttt gttccaaaat atcatctttg ccggcatagt cgaagtttgg caattcgtca
    14821 atgatttttt ttactttttc gaacagcgaa attctatctt catcttttaa aatgttcagt
    14881 tcggtggtgt cgattcctgt ttcatcataa aaaccgatcc ctttccgatg caaaaaatca
    14941 tttacaaaac cggaaatttt tgctttatcg gttgtaaaac ccttaaccga tagtcgcaat
    15001 ttgacttttg aaaaatcttc cggcaacagt tgccaattgt ctatcatttg ttccagttgc
    15061 tgggttaaat aggatatttc gttatcggaa ggcaacacaa caatattttc gacaaaataa
    15121 atgaccgaag cgggtaaatc gaccgaaacc agattattgt catcggtgtc gaaaagcagc
    15181 catcttcttt ttcccgtttc gtttacgtcg agcggaaaag gagaccctgc ataccaaaca
    15241 ctgcccgatg tgtaaggttt gtgtatatgt cccagcacaa cttttgaggg tcggaaagca
    15301 gaaacgctgc ccgaagacaa gggcatgtaa attccttttt cgtagggatt catctcgcga
    15361 cttctggaaa cataatcacc atgaccaata agcacccaat tttttaattc ttcttctcct
    15421 tttctttgaa taaaaaaatt actcaaggcc tcatccattg aaagtttggc atcataaggc
    15481 aaaaaaacaa aaggtatcga acccaaaatt tttatttcgg gagaagaaac aatttccaag
    15541 ttggaagccg taaaatattt ttgttccaac agtaagtcgt gattgccagg aatgaccaga
    15601 aaatgtttgg acgaatgaag tttacaaaaa gcatcaaaat cggcataatt atgaaaatcc
    15661 ttgtcgaaaa ggtctccggc aaccacaagt gtggaggcat cgaaatcgtg catggaattt
    15721 acaagagttt ccaaagccag gtatcgctca ggcgtttcgg ctttggttcg caaatgcaca
    15781 tcggcagtaa tggctatttt cattatacgt ttctgacaaa aagtgtaacg actaaaggaa
    15841 aaaaatcagt aaaagattca ctaaaaaatt gtcttccaaa aacataagtt attcgttagg
    15901 taaactattt tagcacataa aattagcaat tctattcaca ttttcaaaaa aaacttacag
    15961 ttaaactcaa atataatttc cagaaaaaac gtaaagaaac atgtaaatgg aacagaaaaa
    16021 atccctaatt ttgtagcttt cagcaataaa aaaattggac gctcaaaaga aaaaaatatc
    16081 aaaagtcatg gcaaaaacca aaactatgta tgtctgtcag aattgtggag ccgattcgcc
    16141 caaatggata gggcgatgtc cttcctgtgg cgagtggaat acttatgtgg aagaagtggt
    16201 gagcaaagga aatgcggaaa aaatcatcag tggaggtttt accgttactg ctcaaaaacc
    16261 cgttttgttg agcgaagttg aagctattga agaaaaacgc atcaacactt tcaacaatga
    16321 actgaaccgt gttttgggag gaggattggt accgggatcg cttgtattga ttggcgggga
    16381 accgggaatt ggaaaatcga cactggtttt gcaagtggtt ttgcatctca aaggaaagcg
    16441 aacgctatat atttcgggag aagaaagcgt aaaacagatt aaattgcggg cggaacggtt
    16501 gaaatttgat gatcccgatt gttatctttt gagtgaaacc tcactggaac aaatttttgt
    16561 tcatattcaa aatatacaac ccgatttggt aattgtggat tccattcaga ccgtttctac
    16621 cgaaatgctg gatgcttctc ccggttcggt atcgcaggtt agagaatgtg cagccgcttt
    16681 tctgaaattt tgcaaaacca cttccattcc ggtaattttg attggacaca tcaacaagga
    16741 aggcggtctg gcagggccaa aagtactgga acacattgtg gacaccgttc tgcaattcga
    16801 aggcgaccga cattacatgt atcgtatttt gcgttcgata aaaaaccgat ttggcagtac
    16861 atccgaactc ggcatttacg aaatgcgtca agatggtctg cgcgaagttg aaaatccttc
    16921 agaaatgctc ttttcgcagg atcacgaagg attgagcggc attgccattg cttcagccat
    16981 tgaaggcatt cgtccctttc ttatcgaaac gcaggcgttg gtaagttcgg ctgcttacgg
    17041 aatgccacaa cgctcgagta cgggttttga ccttcgtcga ttgaatatgt tgctggcagt
    17101 gctcgaaaag cgtgtgggtt ttaaattgaa tcagaaagat gtatttctca atattgccgg
    17161 cggattacga gtaaatgatc cggctattga tttggctgtg atttctgcaa ttttatcttc
    17221 aaatgtcgat attgccatcg aaaaacaggt ttgtacggcc ggagaagttg gtctttcggg
    17281 cgaaatacga ccgattcatc gcattgaaca acgaattttg gaagccgaaa aactgggatt
    17341 cagaaaaatg attgttcctg ctaataattt aaaaggtctt tcactcaaaa atatcgaaat
    17401 ggaaattata ccggtgaaaa aagtggaaga ggcttttaaa atattgttaa aaaaatgagg
    17461 tgtctggtcg tttaagggga aatgctcttt ttttnnnnnn nnnnnnnnnn nnnnnnnnnn
    17521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17581 nnnnnnnnnn nnnnataatt ttttcaagct ttcggaagag taacaataaa agttgttcct
    17641 ttatctattt gggtagtgaa agaaatattt ccacccgtaa cttcaataat atttttagaa
    17701 attgcaagtc ccagccccat tcctgtagat ttggtagtaa aattcggaat gaacaatttg
    17761 tctctgatat cttcaggtat cccggctcca ttgtcgctaa ttcggatttc gatttgctga
    17821 tgagaagttg aaattttcac cgctattttt ccttgtctat tttcgggaat ggattgtatg
    17881 gcgttcttaa tcagattatt aaaaacttgg attaattgtt ttgggtcagc aaaagtgtaa
    17941 atcggttctt tggaaccttc aaaagaaata tcgacttttt cataattatg tttgaagaac
    18001 tccacttccg acttcagaag ttcttctaac tctacccttt caaattttgc ttccggcata
    18061 cgggcaaaat cggaaaatgc acgggcaatt tgcgaaaggt tatcaatctg ttccaccaac
    18121 atttgagaag ccttatcaaa atactcgttg aatttttcct tatccatttt ttttgttctg
    18181 ctcaattgct gaatagtaag tttcatggga gtaagcggat tgttgatttc gtgagccacc
    18241 tgccgtgcca tcgatttcca tgccgattcc ctttcggaac gtgccaacag gcgagcactc
    18301 tcttccaact cgtccactgt ccggttatat tgagcaacca attggcctat ttcgtcatcg
    18361 taacgataat cgattttttc gtttcttctt cccaaacgta tttctttgag ttttttctcc
    18421 agcatcatca gtggagcgga aatctgattg ccgataagca aggtcagtat aataaccaat
    18481 aaaatgataa caaaatatat gtgaacaatt accgaaataa aactttcaat ttcattctga
    18541 atttcatcct gactgaaaaa ctgagggacg gcaatgtatc cgatttgcag ataatcgcca
    18601 ttgaaaaaat cggtataagc cgtcaaataa ttcaaatggc ctatgttttc atactgattg
    18661 atattggcat cttcagtaaa atatggcttt ggcgcaattc ggttgctgat caattttttc
    18721 gaaaaaatca aaggctgtga actgcccacc aaaattccat cgttgttgta aacatgcaca
    18781 tcggtatgga aagtatagga aagatcctgc aaatccaaat ttaatgtctg ggtattttta
    18841 tcggacaaat ctttgttcca ataatacaaa ttttgaagcg ccttctgaat gtaaattttt
    18901 ttgttctcca gattttgaat ctgttctttc tcgtaattgt tacgaatata gtcaaccgaa
    18961 gaataaaaaa ttccgataaa actcaaaatt aaaagagcaa tgaatgaaat ttggaaccgg
    19021 ttgataaaac tcaaagtaaa tttccttcct tttgcgaaaa aatcatcaac ttgctgaagt
    19081 aaccaacaca aagcaaaaaa agccagaaaa gcataacaaa aatacaacaa atatgcgcct
    19141 gttccatatt gaacattctc tgtcagaacc agataagtat tgcggtcggg ggcataaaca
    19201 taatgacgtc gatcggcata atgaactaca aaataattgg aattctcgaa cggaatccaa
    19261 ttggaaacat cggggtaaac aaaatttcca tttctataga ccaactttcg atgatcgtac
    19321 cgggcaagcg aaacattcaa ctgcgacaaa atggaaggcg gagatgaaat cagtagattg
    19381 ggaaagctat agcttctgaa attctgacgg ggatagaatt ccataaaata acaagtagaa
    19441 tcattgccgg cagtaaggac agaaaaaact cccaaatatg aaatattctt ttcgttcggt
    19501 ataatgttgt agaaatgagt ctgtttgatt tttattcctt tctcctgcaa aaacaactga
    19561 tattccttgc acaaatccga gttttttgaa accgcattaa tatgaacttc aaaacggttc
    19621 caaaaaccac gcaagtaact attgttcaaa tactcgctta aaacttccac cgaatcggag
    19681 tttctcatca actgatttac aaagcggtcg cgacttaact gggcatccaa ttcttccatc
    19741 agaatttctg tcatttggtc gttttcaatg gtaccattca gtgaaagatt ttcggccgca
    19801 attctgtact tgttaaattt cttttcattg gataaaacca ccacatttcc aataaaaaat
    19861 atcgaaaaga acaaaacata aattatgagt ttgacatttc tttttctgga aaaatttaca
    19921 atataaaagc catacaaggg tatccagaga aataagtagg agaaaaaaga aagtttccac
    19981 atttcgggaa aaaccttgaa agccgccatc gctaaaagac aagaaaaaat cgcatcgatt
    20041 gcaaaggcaa ttttcatata ataatgtctg gtcagaaaag tatgaatttt gaaaaacaac
    20101 aaggcaaatc caattcccca cagaagcatt aaaatatgca tccacacggt aaatatggaa
    20161 aaatttttaa agctgagaat aagtatttgc ggcccggaat gataaactac tcctcgtaaa
    20221 acataataaa acaacaaaaa atacaaaggc aacattagca gcaatccgaa acttctcaaa
    20281 aaagaaacac tatttcccaa ctgaacatga cgataaaaca aaaaaaccgt agaaaaaatg
    20341 taaacagtaa gcaaggtcaa atgtaccgtt gaatttaaaa gtggcggaac tgcatacaaa
    20401 taaggcgcaa aaagcttgtt ggcaagtaaa accgaaggaa aattaaaata cagcatcact
    20461 gccaacaacg caccggtaat caaaacataa ccacaaaaat ctttccattg caacggtttg
    20521 gtgcgcgaaa gagaaaatcc tcttgcatac cacacaaaaa acagcaagaa aaacaaaaga
    20581 aaagaaatta agccagccaa agcccaacgt tcatcataca cgggcactgt aggttctgaa
    20641 agtgaaaaca aataatcgcc gtgtgaactg aaaactgcat acttatccaa atttgaacct
    20701 ccgacaattc cgacgttttt gtttaaattg aaacctttgg caaaatcatt gatcagttga
    20761 tcgttttcgt aaggaaaatt attctttatc ttaatcaatg ccaacaattt caacgaatcg
    20821 gcagaaagcg ttttgctcac acaatatgca ttgggcaact gaacaaaatg aagctcaacg
    20881 ctgtcgttca acgaaatact gctgacgtcc aaatgatgat ccgaccaaaa aatcagctca
    20941 ttgccccgat aaacataaaa agatatatca tcgtcgggaa aagaaaaatt aatcagttcg
    21001 ttaaccgaat gattatcaag caaattactc atttgcttca atatacgagc agtagataat
    21061 tctttgctta tcagcttact ttgaaactgg ttgaaatgta cgacacgttg aggcgaagtt
    21121 tgatagaaat attcgcaaaa acatcctgcc aacacaaaaa caagcagaaa tccaaaaaac
    21181 gatattttac cgttttgcct cataaaaaat tttttctctc aagaaatcat ttgtcctttt
    21241 ttcatttcaa gcatgatgat agggttcacc ctttaaaata gtcatggctc tgtagagttg
    21301 ctccacaaaa ataagtctta ccatttgatg cgaaaaagtc attcgtgaaa gcgaaaccat
    21361 attgtttgcc ctgcgataca actcttcaga aaagccataa gctcctccag taacaaaaac
    21421 tatgcgctta ttggaagaat gcattttttt tgataaaaaa tctgcaaatt ctacggaagt
    21481 aaattccgtg cccctctcat ccaacaaaat cacatcatcg ccgatttcga gcttttttag
    21541 aattaaatcc gattctttgg ccgcctgttc ggaagaagac aaattttttg tattttttaa
    21601 ttcgggaata tttatcactt caaaattcaa ataatgtttc aatcgatttt ggtattgttc
    21661 ttgcagtgaa gaaaggtgaa tatcaagagt ttttccaata ttaatcagaa caaatttcat
    21721 cagaatataa tttatttttt aaaggtctga tggaacccac atgtct
//
LOCUS       DAOSIB010000030        21402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_2718_length_21402_cov_12.071813, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000030 DAOSIB010000000
VERSION     DAOSIB010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21402
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2718_length_21402_cov_12.071813"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PLD44_04275"
     CDS             complement(<1..581)
                     /locus_tag="PLD44_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HOF98584.1"
                     /translation="MKKYIISLLALLVMGSIKISAATVVAEPKIDAGDTAWMITATAL
                     VLFMSIPALALFYGGLVRRKNVLNIFMQCMIIVAVISIEWIVCGYSMAFGNSEGFLSP
                     FIGGLDGAFLRNIGIHDLSPYFISHSQIAADGTLVGTIPHLVFVMFQCMFAVITPALI
                     IGAFAERINFKGFLLFTVLWSLFVYNPVAHWVWS"
     gene            complement(638..997)
                     /locus_tag="PLD44_04280"
     CDS             complement(638..997)
                     /locus_tag="PLD44_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOF98585.1"
                     /translation="MKKIEAVIRKSKFEDVKMALYEAGIEWFSYWDITGLGKSKEDQV
                     VRGQIFQSSYIQRRMLSIVVRDINLEKTVNAILDSAWTGDPGDGKIFVSDIEQTYRIR
                     TRESGPEALYNKDSNGI"
     gene            complement(1036..1686)
                     /locus_tag="PLD44_04285"
     CDS             complement(1036..1686)
                     /locus_tag="PLD44_04285"
                     /inference="COORDINATES: protein motif:HMM:NF021230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98586.1"
                     /translation="MKATKLLIICSLTISIPIITKSQNFTVSTDIVSSYVWRGVKYSD
                     ASIQPTLTFSHQNFSIGSWGSAGIDGFMEMDLFVNYAFPFGLSLGMTNYYFPPSKFFD
                     HSASTGSHAYEFNASYKISGFYAAGNYIFNEAGSAGSKGGEKYFEAGYDFKNIRLFAG
                     AGDGWHTPDGSFQAVNIGLSVTRDIQITDSFKLPLKGSAILNPATEQYYLTIGLSL"
     gene            2115..2846
                     /locus_tag="PLD44_04290"
     CDS             2115..2846
                     /locus_tag="PLD44_04290"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOF98587.1"
                     /translation="MSNINKISILFFGLLSQLFCIQLHADNNVKIIYNIKYGLVNGGK
                     AMVEKRDTVFGGKKCIYYRLEGNTTGLTDKIFKVHDTYESIVDAHTHLPYVAVRNAKE
                     KSYRYYNKVFFYHQKDSIFSERTGGMKVPPQLIDILSAFFYMVNVYDMDTLTEGYSLT
                     LHTINGHDIQKMKVKYVNNEIVQTDIGNSLCYVIAPEVKKGKLLKSSSGFKFYIAQNE
                     KIPYQIEIETKVGKIEAMPIEIIRK"
     gene            2948..3949
                     /locus_tag="PLD44_04295"
     CDS             2948..3949
                     /locus_tag="PLD44_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOF98588.1"
                     /translation="MVQTAYSPNPDRYQHSVGYRFCGKSGLKLPQISLGLWHNFGDVD
                     DAEEAEKMIQFAFDHGITHFDLANNYGTPAGSAEINFGKILKKNFASHRDEMVISTKA
                     GHEMWPGPYGDGGSRKYLISSLHQSLKRMNLDYVDIFYSHRYDPNTPIEETMGALSDM
                     VKQGKALYIGLSKYPEEKAREAFRILRENGTPCLIYQDRYNIFSRNIERSHFPLLKEN
                     GIGFIAFSPLAQGILTEKYLHGIPEHSRAALSYGFLQKSQITPEVLAKVQELNQMAHQ
                     RGQTMAQMALAWCLRTSDVNSVIIGASSVNQIQENIDALENISFTDDELWRIGELGA"
     gene            3992..4441
                     /locus_tag="PLD44_04300"
     CDS             3992..4441
                     /locus_tag="PLD44_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98589.1"
                     /translation="MKIFKAFIFFVLLTWVFIGCDYSSRSSIPDATVNFQLNMLVAPY
                     NTFNTPNSYLIFDKRINAIDRIGYGGILLYIDYEGKYCAFDLACPYEVNPNVKVTPND
                     EGKAVCEKCGSVFELLYGIGAPISGPAKEPLKRYHVYLSNNWLYVTN"
     gene            complement(4459..5559)
                     /locus_tag="PLD44_04305"
     CDS             complement(4459..5559)
                     /locus_tag="PLD44_04305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOF98590.1"
                     /translation="MNFILIGCFILVGSYLLMVIFFIIGWKRLSEFELSPHRMNGKFP
                     VSVVVSCHNEEEHLPRLLSALLRQTFSDFELVLVDDHSTDGTYKIMQEFSSAFSNMKF
                     FPSEIKGKKNALQKAVNIANGELMITTDADCEPCESWIETICNFYFQYRPDLIIAPVK
                     LKSNSKIFPQLQKFEFASLVASGAGAAAFGKSILCNGANLAFPKKIYSIHVNDLHNEL
                     LSGDDMFLLESVKKSKGEIRFLKSTLAMVETKTAENLRDFFRQRKRWSSKFSAYRDKD
                     VITVALLVFLTSFFLLCLFILSLITPRMWKYFLVCFMVKYLIDGYFLYSVRKFFDLRS
                     VWFYAFLLSLIYPFYVLITAVSGVFSGQKSWK"
     gene            complement(5883..6377)
                     /gene="tpx"
                     /locus_tag="PLD44_04310"
     CDS             complement(5883..6377)
                     /gene="tpx"
                     /locus_tag="PLD44_04310"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOF98591.1"
                     /translation="MATVKFAGTDVHTNGELPAVGSKAPDFTLVGKDLQEIHLKDFAG
                     KRVVLNIFPSLDTSTCATSVRKFNQWVSGKNNVVVICVSKDLPFAQSRFCGAEGLDNV
                     ITASDFRYNTFATDYGVLLVDGPLKGLMARAVVAIDENGKVIYNQLVDEISNEPNYDI
                     KVLD"
     gene            6477..7676
                     /locus_tag="PLD44_04315"
     CDS             6477..7676
                     /locus_tag="PLD44_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98592.1"
                     /translation="MKLSLLHKLGFFVSIVFLLLSCKDSHAYRVDSAFSTYLYTFQSE
                     AAKRGQVFNFQQEGLIMEFADLTDNKAGLCHYEKPIRIEIDRTYWNDISKYAGAEYMK
                     EDVIFHELGHGFLGREHLNDVLENGDWKSMMCGGQKVNNRAWNINYRGIRRAYYLDEL
                     FNQNTPSPVFSTENFSIDTSAYTLKVRKSFDTALDAGWKIENTSEYETSIDNRRLKFE
                     SKVDPVYFVYAKTNVDVQSDFIYQLQIECKSKNDSDQYGLIFGSVPDQGASYVQKSLE
                     YFTINNLQKMYMGNTTWYSFYTELTKPCIIPSGNNRMKIVKSGEMLYYFINDVYCYCS
                     EMEVKDSGFYFGFIVPPKSTVWLDNLIVSQQITSQSPVSKIREFLVFDAQVVSEPKFR
                     MKCIKGK"
     gene            7725..9407
                     /locus_tag="PLD44_04320"
     CDS             7725..9407
                     /locus_tag="PLD44_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOF98593.1"
                     /translation="MSKFVIYQVLPRLFGNYNSNNKYNGSIEENGCGKFNSFTTKALK
                     EIKDLGVTHIWYTGIFEHATQTDYSAYGIRKDFPCLVKGKAGSPYAVKDYYDVDPDLA
                     ENVPDRMKEFEDLIHRTHKEGMKVLIDFVPNHVARQYFSDAKPENVVDLGEHDHPEWA
                     FSPLNNFYYLPGQPFRPNFDIDGYNEFPAKVTGNDQFTPSPTLNDWYDTVKLNYGINY
                     MEGMQKQFDPIPDTWNKMLDILLFWASKKVDGFRCDMAEMVPVEFWNYAISRVKEKYP
                     KIIFIAEIYKPAEYRNYLANGKFDYLYDKVGMYETLRNVTSRNYPARNITYAWQQLNG
                     IEDHMLNFMENHDEHRIASGFFAGDGSYAQPAMIVAATLTKAPVMIYFGQELGELGMD
                     NEGYSGLDGKTTIFDYWGVKSVQAWANNGKFDGEKLTAEQKELRQFYQKLLNITLQEK
                     AITEGVMYDLEYANFNNPHFDTHEQFAYIRKFKDEMLLFVLNFDDKELYTEVRIPLEA
                     FQYLELMPGCQYKAVNLLDEVETIEQITLIPDMIFKISIPAWKGKIFKFKKL"
     gene            9517..9987
                     /locus_tag="PLD44_04325"
     CDS             9517..9987
                     /locus_tag="PLD44_04325"
                     /inference="COORDINATES: protein motif:HMM:NF025425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4251 domain-containing protein"
                     /protein_id="HOF98594.1"
                     /translation="MKAYFGLFAGMALLLISCSGVKTTPESVKSLTQKVENKNFTVKV
                     QYAQPLSGKQIFLTSEYALTVKNDSAEAFLPYFGVAYSAPYGGDGGIRFNEPMTDFSI
                     MPNKRNNGWDIQFKVKAPEDYYEFFLKIFNDGSANFTVNSNRRQSISFMGEVKN"
     gene            10554..12050
                     /gene="zwf"
                     /locus_tag="PLD44_04330"
     CDS             10554..12050
                     /gene="zwf"
                     /locus_tag="PLD44_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOF98595.1"
                     /translation="MLVIFGSNGDLARRKLLPSIFQLHADRLLPESFAVIGTGSQEKE
                     QSVFLQEVEESLKKYVKSDSVNDDQTVREFLKKVYYLPVRNQVDEDFGKLKEYLHSLS
                     AKLNIPRNIIYYFSIPPFLYEVVASNLVKYGLNRQKDGWKRIIVEKPFGYSYETALDL
                     EKKLHAGFQEDQIYRIDHYLGKETVQNIMVTRFANGFFEPIWNSKYVDRVEITAAEKI
                     GVGNRGGYYDTAGALRDMVQNHLLQVMAIVAMEPPAVFDSDSIRNETVKVLQALRPID
                     EEHVAENVVRGQYIETMVDDVLQKGYRQEKGVASNSRTETYVAMKLFIDNWRWADVPF
                     YIRTGKQLPVQVTEVVIHLKPAPHQLFKQRCITQSTNMIILRLNPDAGIAIDFGMKIP
                     GAGYKVQNVNMAFHYSDLAHSKIPEAYERLILDCMMGDSTLYARADALRASWKFVDPI
                     LNAWKNNPEIPLYFYECNTWGPNEANALFGDKRLKWRPPFNKFKTNEE"
     gene            12075..12827
                     /gene="pgl"
                     /locus_tag="PLD44_04335"
     CDS             12075..12827
                     /gene="pgl"
                     /locus_tag="PLD44_04335"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOF98596.1"
                     /translation="MNPYDILVFNTPEEAAKAIAQLISNRITEKQNSLSPFNLAISGG
                     KTPQTLFNILANDFAEKIPWHQLRLFWVDERCVPPTHPESNFGMTNETLLKKVPLPKK
                     NVFRMKGETVPEEEAIRYQSLLTKELPSKNGIPVFDLILLGMGNDGHTASIFPKDINL
                     IDSHLSVAVTQHPDTGQKRITLTGPVLKQADEIIFFVTGASKATILKEIILNEPSAKK
                     YPAFHIYSAEGKTSFYLDKEAAALLPKELPLL"
     gene            complement(12921..14186)
                     /locus_tag="PLD44_04340"
     CDS             complement(12921..14186)
                     /locus_tag="PLD44_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HOF98597.1"
                     /translation="MFGLVDCNNFYVSCERVFNPSLIGRPVVVLSNNDGCVISRSNEA
                     KALGIPMGQPAYQLKELIEKQNVAVFSSNYVLYGDMSHRVMTTLASFVPDVEVYSIDE
                     AFLLLDGFEKQNLKELGRKIVETIVRNTGIPVSMGIAPTKTLAKVANKFAKKFIDYKG
                     LCLIDTDEKREKALQLTAIEDVWGIGRQYSKMLAYQGIRTAWDFTQKSKAWVRRNMSV
                     TGERTWLELQGNPCIEMEHVAAKKSICTSRSFGERLTEYAPVSEAVANFAANCAEKLR
                     KQHSKAMLLTVFIHTNPFSTSQLQYFNQKTVPLPVPTNDSAELIHHALKALKAIFAEG
                     YLYKKAGVFVGDIVPETPLQTDLFDTRDREKFGKAMQVMDSLNATYGKHKVRIAAQGF
                     GRNWKLKNEKLSPFYTTRLKDILEVKATE"
     gene            complement(14239..14712)
                     /gene="umuD"
                     /locus_tag="PLD44_04345"
     CDS             complement(14239..14712)
                     /gene="umuD"
                     /locus_tag="PLD44_04345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOF98598.1"
                     /translation="MKADKIATSGYIDFYSVNTESAFYLPIADGGIAAGFPSPAQDYI
                     DVKIDLNRELIANPSSTFLGRVKGTSMKDAGIEDGDILIIDKSIEPQDGDMAVCFLDG
                     EFTLKYIHMEKDAVYLMPANPKFKPIKVTEENHFCIWGIVTYSIKHHKQKQGKTK"
     gene            complement(15340..15412)
                     /locus_tag="PLD44_04350"
     tRNA            complement(15340..15412)
                     /locus_tag="PLD44_04350"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15377..15379),aa:Gly,seq:ccc)
     gene            complement(15528..16103)
                     /gene="rsxA"
                     /locus_tag="PLD44_04355"
     CDS             complement(15528..16103)
                     /gene="rsxA"
                     /locus_tag="PLD44_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOF98599.1"
                     /translation="MTYILIFISAVFVNNIVLSQFLGICPFLGVSKKINTALGMSGAV
                     VFVMTIATIFTYIIQKSILEAYNIAFLQTITFILVIAALVQLVEIILKKISPALYQAL
                     GVFLPLITTNCAILGVAILVVQKNFNLLESVVFAISTSIGFGLALILFSGIREQLSMI
                     KVPKKMEGAPIALLTAGLLALAFMGFSGIVK"
     gene            complement(16167..16757)
                     /locus_tag="PLD44_04360"
     CDS             complement(16167..16757)
                     /locus_tag="PLD44_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOF98600.1"
                     /translation="MSNSKVKIILNGLITENPIFVLILGMCPTLATTSSAINGFSMGL
                     ATSFVLIGSNVAISLLKNLIPDKVRIPAYVVVIATFVTVVEMLMQAYLPALFESLGIF
                     IPLIVVNCIVLGRAEAFASKNSVIDSSLDGIGIGLGFTFALTLLGVVRELLGTGKIFS
                     LSVLPEKYGMLVFVLAPGAFIALAYLIAIMNKIRKA"
     gene            complement(16812..17438)
                     /locus_tag="PLD44_04365"
     CDS             complement(16812..17438)
                     /locus_tag="PLD44_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOF98601.1"
                     /translation="MAKLTSSFKNMVISLLSITVFSALTLGSVYTLTKEPIEKSKTAK
                     LQKALKEVLPTFEHLDTAIKVPVNNVDTLNLYKAYDKNNVFVGAAVEANSKNGFGGEI
                     KIMIGFDKEGNIINYSVLEQKETPGLGTKMVDWFKMSNTKHDIRGKNPAKHRLLVSKD
                     GGEIDAITAATISSRAFLEAVNKAYQAYANNPKAPKNHDVSSGASNIN"
     gene            complement(17431..18399)
                     /locus_tag="PLD44_04370"
     CDS             complement(17431..18399)
                     /locus_tag="PLD44_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOF98602.1"
                     /translation="MNKLIVSPAPHVHSGNSIRKEMVSVLIALIPAYLVALYYFGVGA
                     IIVSVTSVISCVIFEYLIQKFLIKGENTVSDYSAVLTGLLLSFNLPSNLPVWIVVIGA
                     LVAIGVGKMSFGGLGNNPFNPALVGRVFLLISFPVQMTSWPRPMAWNTSYLDAETSAT
                     ALSALKEHLGNIPSLFDMFVGNMGGSMGEVSALALLLGFVYLLFKRTITWHIPVSILL
                     TVFIMTGIFYLVNPQTYVSPIIHLLSGGLMLGAIFMATDFVTSPMTKMGMTIYGIGIG
                     IITVVIRMFGSYPEGVSFAILIMNAFTPLINTYIKPKRFGEVRKNG"
     gene            complement(18418..19755)
                     /gene="rsxC"
                     /locus_tag="PLD44_04375"
     CDS             complement(18418..19755)
                     /gene="rsxC"
                     /locus_tag="PLD44_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOF98603.1"
                     /translation="MKTFKMGGVHPPENKFSAGKKTKIISLPQQVIIPLRHYSGNPSV
                     AEVSRGEIVKVGQLIGRANGFISANVHSSVSGKVSKIDNAMDAFGYKFPAVFIDVIGD
                     DWLDEVDLSPEVKRVCNLEPHEIIKKVAEAGIVGLGGATFPTHVKLSIPQGKKAEVLI
                     INGAECEPYLTCDHQLMLEKGEEIIIGISILMKAIRVKKAIIGIESNKMDAVLNLEKW
                     AKTMEGIEVVPLKVKYPQGSEKQLIQALTGREVPSGALPIEVGAVVQNVATTFAVYEA
                     VQKNKPLIERIITVTGKSVPEPGNYWVRIGTPFTHIIEETGGMPQDTGKVVAGGPMMG
                     RALPSLDLSVAKGTSSILFIPSSESKRGKVFNCIRCAKCVFHCPMGLEPYLLMNQGEK
                     QMWENMEENRVMDCIECGSCSFICPSNRPLVDYIRYGKSTVGTMIRNRKTQKS"
     gene            complement(19815..20678)
                     /locus_tag="PLD44_04380"
     CDS             complement(19815..20678)
                     /locus_tag="PLD44_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HOF98604.1"
                     /translation="MNLILASVVSLGGIGALSAVILYFIAQKFHVEEDARIDEVEAVL
                     PAANCGGCGFPGCRAFAAACVTADTLDNLSCPVGGTATMEKVADILGKKAAAVVPTIA
                     VVRCGGTCEVREKTNSYDGVKSCTIVHNLYGGETGCSWGCLGLGDCEVSCTFDAIHIN
                     PETMLPEVDEDKCTACGACVKACPKNIIELRRKGPKSRRIYVNCVNKDKGAVNKKVCK
                     VACIGCSKCFNVCPYEAIEMGNNLAYIDSEKCKLCRKCVEVCPTNAIVELNFPPRKTK
                     IEEPSTTGTEK"
     gene            complement(20718..21137)
                     /locus_tag="PLD44_04385"
     CDS             complement(20718..21137)
                     /locus_tag="PLD44_04385"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOF98605.1"
                     /translation="MQQIEHIGIVQHIEGSKAQILIIQQSACSACHAKSACTVMEEEK
                     KIVDVVVTDSSIKEGDQVVIFGRNSMGLQAVLLAFIFPLLLIVLTLIISNYFQQNETI
                     SGIIALCSIFPYYGILSLFNHKLKRKFEFHVKKESLT"
BASE COUNT         6975 a         4098 c         3869 g         6460 t
ORIGIN      
        1 gaccaaaccc aatgagccac aggattgtaa acaaatagcg accacaaaac ggtaaaaagc
       61 aagaaacctt tgaaattaat acgttcggcg aaagcaccaa tgataagtgc gggtgtaatt
      121 accgcaaaca tacattggaa catcacaaaa accaaatgag gaatagttcc aaccaaagtg
      181 ccgtctgctg cgatttgcga atgagaaata aagtaaggac ttaaatcatg aatgccgata
      241 tttcttaaaa acgccccatc caatccgcca ataaaaggag aaagaaatcc ttcagagttt
      301 ccgaatgcca tgctatatcc gcaaacaatc cattcaatgc tgattacggc cacgataatc
      361 atacattgca tgaatatgtt gagtacattc tttctgcgta ccaagccacc gtaaaaaaga
      421 gcaagtgccg gaatactcat aaataataca agtgctgttg ccgtaatcat ccacgccgta
      481 tctccggcat ctattttcgg ttcggcaact accgttgcgg ccgatatttt aatactgccc
      541 attaccaata gggccaacaa ggaaattata tattttttca tttttttata cctttaaaaa
      601 ttaatacctt ataaacaatt gttttaaaaa acgaaactta tattccgttt gaatctttat
      661 tgtacaaagc ttccggtccc gattcacgag tacgaatgcg gtaagtctgc tcaatatcgg
      721 aaacaaaaat ttttccatct ccgggatcgc cagtccatgc cgaatccaaa atggcattca
      781 ccgttttttc gagattgatg tcgcgcacca caatcgacaa catacgccgc tgaatataac
      841 tcgactgaaa aatttgtcct cttaccacct gatcctcttt cgatttgcca agtccggtaa
      901 tgtcccaata ggaaaaccac tcgatgccgg cctcataaag tgccatcttt acatcttcaa
      961 atttagattt tctgatcact gcttcaattt ttttcataat cgtatgattt taataaaatg
     1021 ttgaattata aatatttata atgataagcc aatggttaaa taatattgtt ccgttgccgg
     1081 atttaaaatt gcagaacctt tcagtggaag tttgaacgaa tcggttattt ggatatctct
     1141 tgtaacgctc aatccgatat ttactgcctg aaaacttcca tcaggagtgt gccaaccgtc
     1201 gcctgcacca gcaaataatc ttatattttt aaagtcatat ccggcttcaa aatacttttc
     1261 gccgccttta gaccctgcac tcccggcttc attgaaaatg taatttccag cagcataaaa
     1321 tcctgaaatt ttatatgaag cattgaattc ataggcatgt gaaccggtac tcgcggaatg
     1381 atcaaaaaat ttggatggcg gaaaataata gtttgtcatt cccaatgata aaccaaacgg
     1441 aaaagcataa ttgacaaaca aatccatttc catgaagcca tcaatccctg ccgaacccca
     1501 cgaaccgata gaaaaatttt gatggctgaa agtcagcgta ggttgaatgg aagcatcgga
     1561 atatttaact ccacgccaaa cgtacgaact aacaatatcg gtagatactg taaagttttg
     1621 agatttcgtt ataattggaa tagaaattgt aagggaacaa ataatcaata actttgttgc
     1681 tttcataact ttcaaatttt aattttagaa tttttctttt ttccgtaatt ttgagtttat
     1741 atatacataa tgtttatttt tcaaataaaa tacaatcaaa atgttttatt tacatggcaa
     1801 aattaatcat aatgataaat taaattcaaa ataaaataca ttttatttca aaaaatattt
     1861 ttataattac aaattgtttt cggaattata aaaataatat catattgttt ttttttaact
     1921 caaatagttt tcagaatgat atttaataat atttaaggac aagcattttt tattttttat
     1981 ctccaaatcg aaagtttgaa actcatagct tgaaaaaggc aaaaaagcaa aagaattttt
     2041 tgaaaaacgc attaaaaaat atgaactaaa ataagcacat ttcatcttaa aaaaatttcg
     2101 ataaaacatt ctatatgagc aatataaata aaatttcaat actatttttt ggattgcttt
     2161 ctcaattatt ctgcattcaa ttgcatgcag acaataatgt gaaaatcatt tacaacataa
     2221 aatacggact tgtcaatggc ggaaaagcaa tggttgaaaa gagagatacg gtatttggcg
     2281 ggaaaaaatg tatttattac cgattagaag gcaacacaac cggattaaca gataaaattt
     2341 tcaaggtgca cgacacttac gaaagcatag tcgatgcgca tactcatctc ccttacgtgg
     2401 cggtgagaaa tgcgaaagaa aaatcttacc gatattataa taaagtattt ttttatcatc
     2461 aaaaagattc aattttcagt gaacgaaccg gaggaatgaa agttccacca caattgattg
     2521 atattctatc ggcatttttc tatatggtaa atgtatacga catggacacc ctcacagaag
     2581 gatacagttt gacattgcac accatcaacg ggcacgatat acaaaagatg aaagtgaaat
     2641 atgtaaataa tgaaattgta caaacagaca taggaaacag tctttgttat gtcattgctc
     2701 cggaagtgaa aaaagggaaa ttgttgaaaa gctcttccgg ctttaaattt tacattgcac
     2761 agaatgaaaa aattccttat cagattgaaa ttgagacaaa agtggggaaa attgaggcta
     2821 tgccaataga aatcataagg aagtagaaaa ttaaaaatac tactgaagtc aaaaagataa
     2881 caataatatc gttttaccac ataaaatttg tatttttacc gctgaattat ttacaaatta
     2941 attaactatg gttcaaacag cttattctcc caatcccgat cgctatcaac attcggtcgg
     3001 ataccgtttt tgcggaaaaa gcggattgaa gttgccgcaa atttcactcg gcttgtggca
     3061 caactttgga gatgtggacg atgcggaaga agccgaaaaa atgattcagt ttgcctttga
     3121 tcacggcatc acacatttcg atttggcaaa caattacgga acacctgccg gcagtgccga
     3181 aataaatttc ggaaaaatat tgaaaaaaaa tttcgcttct catcgtgatg aaatggttat
     3241 cagtaccaaa gccggacatg aaatgtggcc aggcccttat ggcgatggcg gttcgcgaaa
     3301 atatctgata agcagtctcc accaaagtct gaaaagaatg aatctggatt atgtggatat
     3361 tttttattcg caccgatacg atccgaatac acccattgag gaaactatgg gagccctttc
     3421 cgatatggta aaacaaggaa aagctcttta cattgggctt tcaaaatatc ctgaagaaaa
     3481 agcccgggaa gcattccgga ttttacgcga aaacggcaca ccctgtttaa tctatcaaga
     3541 tcggtacaat attttcagcc gaaatattga acgctcacac ttccctttat tgaaggaaaa
     3601 cggaatcgga tttattgctt tttctccatt ggctcaggga attttaaccg aaaaatattt
     3661 gcacggaatt cctgaacatt caagagctgc tttgtcctat ggctttttac aaaaatcgca
     3721 aatcacgccc gaagtattgg ccaaagtgca agaactgaac caaatggctc atcaaagagg
     3781 tcaaactatg gcacaaatgg cgctggcttg gtgtttgaga acttccgacg tcaattcggt
     3841 tatcattggt gccagctcgg taaatcaaat tcaggaaaac attgatgctt tggaaaatat
     3901 ttcgttcacc gacgatgaac tctggagaat cggtgaattg ggagcttagc gcatcaatta
     3961 ccctgactca ataaggaaat ttaactgcaa aatgaaaatt ttcaaagcat tcatcttttt
     4021 cgtattgctc acatgggtct tcatcggttg cgactactca tcaagatctt ccattcctga
     4081 cgccacggtg aattttcagc tcaacatgct cgtcgcacct tacaacacat ttaatacgcc
     4141 aaattcatat ctgattttcg acaaacgtat caacgcaatt gaccgaattg gttacggagg
     4201 gattttactc tatatcgact acgaagggaa atattgcgcc ttcgacctcg cttgccctta
     4261 tgaagtaaac ccaaatgtta aagttacgcc aaatgatgaa ggaaaagctg tatgcgaaaa
     4321 atgtggaagt gtttttgaac ttctttacgg tattggcgct cccatttccg gacctgccaa
     4381 agaaccactg aaacgttacc atgtgtattt gtcaaacaac tggctttacg ttacaaactg
     4441 acaatcgaga gttttaaatt atttccaact tttttgaccg gaaaaaacgc cggaaacagc
     4501 cgttattagt acataaaaag gataaatcag cgaaagcaga aaagcataaa accaaactga
     4561 acgtaagtcg aaaaattttc gtaccgaata aagaaaataa ccgtcaatca gatatttgac
     4621 cataaaacaa accaaaaaat atttccacat tctcggcgtt attaaagaca aaataaaaag
     4681 gcacagcaga aaaaaactcg tcaaaaaaac caatagagct acagtaataa catctttatc
     4741 tcgataagca gaaaatttgg aagaccatcg cttgcgctgt cgaaaaaaat cgcgcaaatt
     4801 ttcggcagtt tttgtttcta ccatcgccaa agtcgatttt aaaaatctga tttctccctt
     4861 actttttttc acactctcca acaaaaacat gtcatctccc gaaagcaatt cattatgaag
     4921 atcatttacg tgaatcgaat aaatcttttt tggaaaagca agattcgctc cattacacaa
     4981 gatcgatttc ccaaaagctg ctgctcctgc tccggaagcc accaaactgg caaattcaaa
     5041 cttttggagt tgaggaaaaa tttttgaatt actttttagc ttaaccggag caataattaa
     5101 atcgggacga tattgaaaat aaaaattaca aatagtctca atccaacttt cacaaggttc
     5161 acaatcggca tcggttgtaa tcatcagttc cccgtttgca atatttacgg ctttctgcaa
     5221 agcatttttc tttcctttaa tttctgatgg aaaaaacttc atattggaaa atgctgacga
     5281 aaattcctgc attattttat aggtcccatc cgttgaatga tcatcgacca gcaccagttc
     5341 aaaatcggaa aaagtctgac gcaacaaagc cgaaagcaac cgaggaagat gctcttcttc
     5401 attgtggcaa ctgacgacca cagaaacagg aaattttcca ttcattcgat gaggagaaag
     5461 ttcaaattca gacaatcttt tccaacctat gatgaaaaaa atcaccataa gcaggtaaga
     5521 accaaccaaa ataaaacaac caattaggat aaaattcatt gttcaaaaaa tgatttaaac
     5581 caattaatta atagaccttt tatgagaata acagataaaa agttcttttt caaaatattt
     5641 gataaaagaa ctttttttgc tttgatgaaa caaatgcttt actttgattt tccttttatc
     5701 aaaagccaca aaaatataac attttactga aaaacaaaga caaacaaatt atgttaaaaa
     5761 ttaaacaggt tgtcaataag ttgaaaatga aaaagggtaa tttgtttatt ttcaacgcaa
     5821 aaacaccctc tcccacaaaa taagtgaaag agggtgtttt ttaaaagaaa atagaccttt
     5881 tatcagtcga gcacttttat atcgtaattc ggctcgttgc tgatttcgtc aaccaactga
     5941 ttgtaaataa ctttaccatt ttcatcgatg gctactacgg cacgagccat tagtcctttc
     6001 agcgggccat caaccagcag aaccccataa tcggtagcaa aagtattgta tctgaaatcg
     6061 gaagccgtga taacattgtc aagtccttct gctccacaaa atctgctttg ggcaaagggc
     6121 aaatcctttg aaacgcaaat taccacaaca ttgtttttcc cggaaaccca ctgattgaat
     6181 tttctaacgg aagttgcaca agtgctggta tccaagctgg gaaaaatatt cagcacgacg
     6241 cgtttgccgg caaaatcttt caagtgaatt tcctgaagat cttttccaac caatgtgaaa
     6301 tcgggagctt tggaacccac tgcaggcaat tcgccattgg tatgtacgtc tgttcccgca
     6361 aatttaactg tagccataaa attaagtatt aaaatgttta aaaattattt tcattttaaa
     6421 ctaataacaa agctatgacg ttttttgttt aaatttgtgt ggaaaaattt ttaacaatga
     6481 aattatctct gctccataaa cttggttttt ttgtaagcat tgtttttcta ctcctttcct
     6541 gtaaagatag ccatgcctat cgagtagatt cagccttttc cacttatttg tacacctttc
     6601 agtccgaagc cgcaaaaaga ggtcaggttt ttaattttca acaagaaggt ttgatcatgg
     6661 aatttgccga tttaactgac aacaaagcag gactttgtca ttatgaaaaa ccgatacgaa
     6721 tagaaattga ccgaacttac tggaatgata tttcaaaata tgccggggct gaatatatga
     6781 aagaagatgt tatttttcac gagttgggac atggtttttt gggccgtgaa catttgaatg
     6841 atgtgttgga aaatggcgat tggaaatcaa tgatgtgtgg aggccagaag gtgaacaata
     6901 gagcatggaa tataaattat cgcggcatca gaagagccta ttatctcgat gagcttttca
     6961 atcaaaatac cccttctcct gtattttcta cagaaaattt ttcgatagat acttccgcct
     7021 atactctcaa agttcgtaaa tctttcgata cagcgcttga tgccggttgg aaaatcgaaa
     7081 acacttccga gtatgaaact tccatcgata atcgccgtct gaaatttgag tcaaaagtcg
     7141 atcccgttta ttttgtttat gccaaaacga atgtcgatgt acaatcagat tttatttatc
     7201 agcttcagat agaatgtaag agcaagaacg attccgacca gtatggtttg atttttggtt
     7261 cagtgcccga ccagggtgca tcttacgtac aaaaatcgct ggagtatttt accattaata
     7321 atttgcagaa aatgtatatg ggaaatacaa catggtacag tttttatacc gaattaacca
     7381 agccatgcat tattccatcc ggaaataacc ggatgaaaat agtaaaatcc ggcgaaatgc
     7441 tttattattt tatcaatgat gtgtattgtt actgttcaga aatggaagtg aaagacagcg
     7501 gtttttattt tggattcatt gttccgccaa agagtacggt ttggttggac aatttaatcg
     7561 tatctcagca aatcacttca cagagtccag tatcaaaaat tcgagaattt cttgtatttg
     7621 atgctcaagt tgtttctgaa ccgaaattta ggatgaagtg tattaaaggc aaataaaaat
     7681 tagaagaaaa acttttttgt ttaaaaccaa caaattcagt cattatgtca aaatttgtaa
     7741 tataccaggt gttaccgcgt cttttcggta attataattc caacaacaaa tacaatggaa
     7801 gcatagaaga aaatggatgc ggaaaattta acagctttac aacaaaagcc ctgaaagaga
     7861 taaaagactt gggagttacc catatttggt acaccggaat ttttgagcat gctacccaaa
     7921 cagattattc tgcttacggc atcagaaaag attttccttg tctggtaaaa gggaaagcag
     7981 ggtctcctta tgccgtaaaa gattactacg atgtcgatcc cgatttggct gaaaacgtcc
     8041 ccgacagaat gaaagaattt gaagatttga ttcaccgaac ccacaaggaa ggcatgaaag
     8101 ttttgattga ttttgtgcct aatcacgtgg cgcgacaata tttttcggac gccaagccag
     8161 aaaatgtcgt tgacttgggc gaacatgatc acccggaatg ggctttttct ccgctcaata
     8221 atttttatta tttgcccggt caacccttcc gtcccaattt tgatattgat ggctacaatg
     8281 aatttccggc aaaagttacc ggaaacgacc aattcacccc ttctccaacg ctgaacgact
     8341 ggtacgatac ggtgaagctg aattatggca taaattatat ggaaggaatg caaaaacaat
     8401 tcgatcctat ccccgataca tggaataaaa tgctggatat tttgcttttc tgggcatcga
     8461 agaaagttga cggctttcgt tgcgacatgg ccgaaatggt gccggtagaa ttttggaatt
     8521 atgccatcag cagagtaaag gaaaaatatc cgaaaattat ttttattgca gaaatctata
     8581 aaccggccga gtatcggaat tatctggcaa acgggaaatt cgattatctt tacgataaag
     8641 taggtatgta cgaaacgctt cgaaacgtaa ccagccgaaa ttatcccgcc cgcaacatca
     8701 cttatgcttg gcaacagctg aacggtatcg aagatcacat gcttaatttt atggagaatc
     8761 atgatgaaca tcggatcgct tccggatttt ttgcaggcga tggcagttat gcgcagccgg
     8821 ctatgatagt ggcagcaact ctcaccaaag ctcccgttat gatttatttc ggtcaggaac
     8881 ttggtgaatt gggaatggat aacgaaggat acagcggact cgacggaaaa acaacaattt
     8941 tcgattattg gggagtaaag tccgtgcagg catgggcaaa caatggaaaa tttgatggag
     9001 aaaagttaac tgcagaacaa aaggaactgc gacagtttta tcaaaagttg ttgaatatta
     9061 cgctgcaaga aaaggctata acagaagggg taatgtacga tttggaatat gccaatttca
     9121 ataatcctca tttcgacaca cacgaacaat ttgcctacat tcgaaagttc aaagacgaaa
     9181 tgctgctgtt tgttctgaat ttcgacgata aggaacttta caccgaagtg cgtattcctt
     9241 tggaggcttt tcaatacttg gaacttatgc cgggttgtca gtacaaagca gtcaatttac
     9301 ttgatgaggt ggaaacgatt gaacaaatta cactcatacc cgatatgatt tttaaaattt
     9361 ccatcccggc ttggaaaggt aaaattttta aattcaaaaa gctgtgaatc aggaagtttt
     9421 tgttataaat ttttttgaca aaataatgga aatggaatat tttttgatga attaaaactg
     9481 acaattattc ttgttttact tataaatctg tacactatga aagcttattt tggcttgttt
     9541 gcaggcatgg ctttattgct gatttcctgc tccggagtta aaaccactcc cgaaagtgtt
     9601 aaatctctta cccaaaaagt ggaaaataaa aattttacgg tgaaagttca gtatgctcaa
     9661 cccctctcag gaaaacagat ttttttgacg tccgaatatg cgctgacagt taaaaatgac
     9721 tccgccgaag catttttgcc ctatttcgga gtggcttatt cggctcccta tggtggtgat
     9781 ggcggtattc gtttcaatga acccatgacc gactttagca taatgcccaa taaaagaaac
     9841 aatggttggg atattcagtt taaagtgaaa gcgccggaag attactatga attttttctg
     9901 aaaatcttca atgatgggag tgccaatttt acggtaaatt caaatagacg gcaatcaatt
     9961 tcgtttatgg gagaagtgaa aaattagcta atgctaattg tagaaacttc tttccttacg
    10021 gtcgaagaag atatacaaat taaccctata tggaatgctt ctgcttgaaa tttgaaaaat
    10081 atcagaaaag gtcaactttt ttattttttt gattgaatgt gtcctaaaat tttataagcg
    10141 tcccatttaa ttaaaagagt ttttaaaaat tgattgttta gtcaacgtaa atcctccgtt
    10201 cagtcagaat tatttttaaa actgctgaaa tagaggatag aaagtgaaga attgttattt
    10261 taggaaactg tttgataaca gagagcgttg ataaaatttt ttcctcattc gacattaaaa
    10321 atcattttaa aaaacacatc atttcaataa acttttgttc aattgggttg tttagacaat
    10381 gataagatga aaaaagtttt gcaatgttac tttgtattca agacaatgca ataaattcga
    10441 ttttttaaaa cgtaaaaaca caaataattg tttatcaatc aaataaaata aaataaaaat
    10501 ataaaatggc tgaacaaaag gtcttatcta caaaaaataa tattgaaggg caaatgctgg
    10561 taatattcgg ttcgaatgga gatttagcca ggcgcaagtt gctgccttca atatttcaac
    10621 tgcatgcgga tcgtcttcta cccgaaagtt ttgccgtaat tggcaccggc agtcaggaaa
    10681 aggaacaatc cgtttttttg caggaagtgg aagaaagtct gaaaaaatat gtaaaaagcg
    10741 attcggtaaa cgatgatcag acagtgaggg aatttttgaa aaaagtctat tacttgcccg
    10801 tcagaaacca ggtggatgaa gatttcggaa aactgaaaga atatcttcat tccctttcgg
    10861 caaaactaaa tattccgcgt aatattattt actatttttc tatccctcct tttctatacg
    10921 aagtggttgc ttccaatttg gtaaaatatg gactgaacag acaaaaagac ggttggaaaa
    10981 ggattatcgt tgaaaagcct ttcggctaca gctatgaaac agcattggac ttggaaaaaa
    11041 aacttcatgc cggctttcag gaagatcaaa tttaccgtat cgaccattat ctgggaaaag
    11101 aaacggtgca aaacattatg gttacacgtt ttgccaacgg tttttttgaa ccgatatgga
    11161 acagcaaata tgtggacaga gtagaaataa cggctgccga gaaaattggc gttggcaata
    11221 gaggcggata ttacgatact gcgggtgcac ttcgcgacat ggtgcaaaat catttgttgc
    11281 aggtaatggc tattgttgca atggaaccgc ctgccgtttt tgattccgat tccatccgaa
    11341 acgaaacggt taaggtgctt caagctttgc gacccattga tgaagaacat gttgccgaaa
    11401 atgtggtgcg tggccaatat atcgaaacta tggtggacga cgtgctgcaa aaaggctatc
    11461 gtcaggagaa gggagttgcg tccaattcca gaaccgaaac gtatgttgcc atgaaacttt
    11521 ttatcgacaa ctggcgctgg gcagacgtcc cattttatat tcgcaccggt aaacaattgc
    11581 ccgttcaggt taccgaagta gttattcatt taaaaccggc tcctcatcag ttgttcaagc
    11641 aacgttgcat tactcagtcc accaatatga ttatacttcg tctcaatccg gatgcaggca
    11701 ttgccatcga tttcggtatg aaaattcccg gcgctggcta caaagttcaa aatgtcaaca
    11761 tggcatttca ttattccgac ttggcgcatt cgaaaattcc ggaagcttat gaaagactca
    11821 ttttggactg catgatggga gattccacac tttatgcccg agccgatgca ctgagagcaa
    11881 gctggaaatt tgtagatcct attttgaatg cttggaaaaa taatcccgaa attccactct
    11941 atttttacga atgcaatacc tggggaccca atgaagccaa tgcacttttt ggcgacaagc
    12001 gtttgaagtg gaggcctcca ttcaacaaat ttaaaacaaa cgaagaataa aagccaatac
    12061 ctattttcaa aaaaatgaat ccatacgata ttcttgtttt caatacgccg gaagaggcag
    12121 caaaagcaat tgctcagctt atttcgaatc ggataaccga aaaacaaaat tcattatcac
    12181 cctttaattt ggctatttcg ggaggaaaaa ctccacaaac actttttaat attttggcta
    12241 atgattttgc ggaaaaaatt ccgtggcacc aacttcgcct gttttgggtt gacgaacgtt
    12301 gcgtccctcc gacgcatccg gaaagcaact ttggaatgac taatgaaacc ctgctgaaaa
    12361 aagttcctct tcctaaaaaa aacgtttttc gtatgaaggg agaaactgtt ccagaagaag
    12421 aagccattcg ttatcaatcg cttctgacaa aagaacttcc atcaaaaaac ggaattcccg
    12481 ttttcgacct gattttgttg ggaatgggaa atgatgggca taccgcttcc atttttccaa
    12541 aagacataaa tttgatcgac agtcatttat ctgtagcggt tacccaacat cccgacacag
    12601 gacaaaaaag aatcacgcta actgggcctg tgctgaaaca ggcagacgaa ataatatttt
    12661 ttgttacagg tgcttcaaaa gcgacaattt taaaggaaat cattctgaat gagccttctg
    12721 caaaaaaata tccggctttt catatctatt cggcagaagg aaaaacttct ttttatctgg
    12781 acaaagaagc cgcagctctt ctacccaaag aattgccatt gttgtaatat tttttgttta
    12841 ttcattgatt tcaaaaatcc gccaattcaa attttgattg ataaaacatc tcaacttgat
    12901 agcaaagaaa gtcaaaaaat ttattccgta gctttgactt ccagaatatc ctttagtcgg
    12961 gtagtgtaaa aaggagaaag tttttcgttt ttcaatttcc agttgcgccc aaaaccttgt
    13021 gcagcaatac gtactttgtg tttcccgtag gtagcattta agctatccat tacctgcatg
    13081 gctttaccga atttttccct gtcgcgcgta tcgaacaaat cggtctgaag cggagtctcc
    13141 ggaacaatat cgcccacaaa aactcctgct tttttgtaca gataaccttc ggcaaaaata
    13201 gctttcaaag ccttcagtgc atgatgaatc aattcggctg aatcgttggt tggaacaggt
    13261 agaggaacgg ttttctggtt aaaatattgc aattgtgagg ttgagaaagg gttggtatga
    13321 ataaaaaccg tcagcagcat agcttttgaa tgttgttttc tcaacttttc ggcacagttg
    13381 gctgcaaaat ttgctaccgc ttccgatacg ggagcatatt cggtgagtct ttcaccaaaa
    13441 cttcgagacg tacaaatgga ttttttggca gctacgtgtt ccatttcaat gcacggattc
    13501 ccttgcaatt cgagccaagt acgttcgccc gttacgctca tatttcggcg tacccaggct
    13561 ttcgattttt gagtaaaatc ccaagccgta cgaataccct gatatgccag cattttggaa
    13621 tattgtctgc caattcccca aacatcttca atggcagtga gttggagtgc tttctcacgt
    13681 ttttcgtcgg tatcaattaa acaaagtccc ttgtaatcta taaatttttt ggcaaattta
    13741 ttggctactt ttgccagtgt tttggtagga gcaataccca tactgacagg aattccggtg
    13801 ttgcgtacga tagtttccac aatttttctt cccaattcct ttaaattttg cttttcaaac
    13861 ccatctaaca acaaaaaggc ttcatctatg gaatacactt cgacatcggg cacaaaagat
    13921 gcaagtgtag tcatcaccct gtgagacata tcaccgtaca atacataatt ggaagaaaaa
    13981 acggcaacat tttgtttttc tatcagttcc ttcaattgat aggcaggttg tcccatcggt
    14041 attcccagtg ctttggcttc gttggaacgg gaaattacgc atccatcgtt gttggacaat
    14101 accacgacgg gacgacctat gagcgacgga ttaaaaacgc gttcacaaga gacataaaaa
    14161 ttattgcaat ctaccaaccc aaacattttg cacatttttt tcttcttttc cctcaatttc
    14221 gggaaaaatt tcttggactt atttcgtttt tccctgcttt tgtttgtgat gctttatgga
    14281 atacgtaaca attccccaaa tgcagaaatg attttcctcc gttaccttaa taggcttgaa
    14341 tttcggatta gccggcatta aatacacagc gtccttttcc atgtggatat atttcagtgt
    14401 aaattcgcca tccagaaaac ataccgccat atcgccatcc tgcggttcga tcgacttgtc
    14461 gataatcaga atatctccgt cttcaatacc ggcatctttc atcgatgttc cctttacccg
    14521 tcccaaaaaa gtactggaag gattggcaat aagttcacga ttcaaatcaa ttttcacatc
    14581 gatgtaatcc tgcgccggac tcggaaatcc tgctgcaatt cctccatctg caataggcag
    14641 ataaaaagca gactccgtat ttaccgagta aaaatcaata taacctgatg ttgcgatttt
    14701 atcagctttc ataaattttt gttgacatga ataaatcgat ttttgtttgt tctttcaaca
    14761 caaagtaaca gatttttttg aaaaaaaagg aaagagcaaa gtaaaagaaa gcataaaaat
    14821 tgaaaaaatc aacaatttat tgtatgtcaa tgcgataaaa aattaggagt tactctttta
    14881 attgtccggt ttacgcggca agattgtaaa tgtttgtgaa tagaaataga aacaatgctt
    14941 cgggaaataa ttcacttata aaaagttaga aattgattgc acaaatggtc atcctacttt
    15001 tcaaattaag atcaggaaat gtcataatat tgagaaaccc gtatgattac atacacaaac
    15061 acgaatgaat ataattttag acatgtgggt tccatcagac cattaaaaaa taaatcatat
    15121 tctgatgaaa agaagtttaa aaattagagt ttttcatccc aaaaaatata atgtactata
    15181 gtctgaagcg ttcgcaaacg ggcttggata agttgttttt tacctagata cttttttaca
    15241 agcaagtgct ttttgcaggt gagccatttc tatttctatt tctatttcaa tatccaataa
    15301 aaaaaggctg tcccaacttt tgagacagcc ccatttttga gcggcaaacg ggactcgaac
    15361 ccgcgacctt tagcttggga agctaaagct ctaccaactg agctactgcc gcaaatagaa
    15421 atacaaaagt aaattaaatt ttataaacga caagaagttt tcaaaaaatc ccttagatat
    15481 ggattataat tcttttgttg tgttaataac atattccccg aaaaaattta ttttacgata
    15541 ccggagaaac ccataaatgc caaagccagc aaaccggcag taagtaatgc tatgggtgct
    15601 ccttccattt ttttcggaac ttttatcatg ctcaattgtt ctctaatgcc cgaaaaaaga
    15661 attaatgcca aaccgaaacc gatggatgta gatatggcaa atactacact ttcaagtaaa
    15721 ttgaagtttt tctgaaccac caaaatagct acgccaagaa tcgcgcaatt ggtagtaatt
    15781 aaaggaagaa aaactcctaa agcctgatat agcgccgggg aaattttttt taagataatt
    15841 tcaaccagtt gtaccaaagc tgcaataacc agaatgaagg taatagtttg taaaaaagcg
    15901 atattgtaag cttccagaat ggatttttga atgatatatg tgaaaatagt agcaatagtc
    15961 attacaaaaa ctactgctcc actcattccc aaagccgtat taattttttt ggataccccc
    16021 aagaatggac agattcctaa aaattgagaa agaacaatgt tgtttacaaa gacggcactg
    16081 atgaaaatta aaatataagt catggcattg taaatattta atcagttcaa caatttaata
    16141 tgtttcaaaa ataaatctat caggttttat gcttttctaa tcttgttcat aattgcaatc
    16201 agataagcga gagcgataaa agctcccgga gccagaacaa ataccagcat tccgtatttt
    16261 tcgggtaata cactcagact gaaaattttt cctgttccaa gcaattctct tactacgccc
    16321 aacaatgtca atgcaaaagt aaatcccaat ccaatgccaa tgccatcaag ggacgaatcg
    16381 ataactgagt tttttgatgc gaaagcttca gccctgccta atacgataca attgacaact
    16441 attaagggaa taaaaatacc gaggctttca aaaagtgctg gtaaatatgc ctgcataagc
    16501 atttccacaa ctgttacaaa tgtggcaata acaaccacat aagcagggat gcgcactttg
    16561 tcgggaataa gattttttaa cagagaaatg gccacattgg acccaattag tacaaatgaa
    16621 gtggccaaac ccatactaaa accgttgatt gcagaagatg tggtagccag cgttggacac
    16681 attcccaata tcaatacaaa aataggattt tcggtgatta aaccattgag tatgatttta
    16741 acttttgaat tactcattgt tttatttttt gcggtaataa acgtaataaa tatataaaaa
    16801 tatgtaatta cttaatttat atttgatgct cccgatgaaa catcatggtt ttttggtgct
    16861 ttgggattgt tggcataagc ctgatacgct ttgttcacgg cttccaaaaa agctcttgag
    16921 ctaatggttg ctgcagtgat ggcgtcaatt tctcctccat ccttgctgac caaaagacga
    16981 tgtttagcag gattttttcc acgaatatcg tgtttggtgt tggacatttt aaaccaatct
    17041 accatttttg tccccaaacc gggagtttct ttttgttcca ataccgaata atttatgata
    17101 tttccttcct tgtcgaaacc aatcataatt tttatttctc ctccgaatcc attttttgag
    17161 ttggcttcta ccgcagcacc tacaaaaacg ttatttttgt cataggcctt gtacaaattc
    17221 agtgtatcga cattatttac tggaactttg atggcagtat ccaaatgttc aaaagtcggc
    17281 aatacttctt tcagtgcttt ttgtaatttg gcagtctttg acttttctat tggttctttg
    17341 gtgagtgtat atactgagcc gagtgttaaa gccgaaaaaa ctgttatcga caaaagtgaa
    17401 atcaccatat ttttaaaaga agatgttaat ttagccattc ttacgtacct ccccaaaacg
    17461 tttaggttta atatatgtat taattagtgg tgtaaacgca ttcatgataa ggatagcaaa
    17521 agatactcct tcgggatagg aaccaaacat tctgataaca acagtgataa taccaatgcc
    17581 aatgccgtaa attgtcattc ccatttttgt catgggagaa gtaacaaagt cggtggccat
    17641 aaagatagct cccagcatta atccgcccga caataaatgt ataattgggg aaacataagt
    17701 ctgaggatta acaagataga aaatgcctgt catgataaat acagttagta aaatactcac
    17761 aggaatatgc caggtaatgg tacgtttaaa gagtaagtaa acaaatccta acagcaaagc
    17821 caaagcagaa acttctccca tagatcctcc catatttcct acaaacatat cgaacaaaga
    17881 gggtatgttt cccaaatgtt ctttcaaagc acttaaggct gtagctgagg tttctgcgtc
    17941 aaggtaagaa gtattccaag ccatagggcg aggccaagat gtcatttgta cgggaaagga
    18001 aatcaataaa aaaactcgac ccaccagggc gggattgaaa ggattgtttc ccagtccccc
    18061 aaaactcatt ttgcctacgc caattgctac caaagctccg ataacaacaa tccatacggg
    18121 cagattagaa ggaagattaa aagatagcaa tagtccggtt aaaacagctg aatagtcgga
    18181 aacagtattt tctcctttga ttaaaaattt ttgaattaag tattcaaaaa ttacacatga
    18241 aatcaccgag gttacactta caatgatagc gccaactcca aaataataca gagccaccag
    18301 atatgcagga atgagtgcga tcagtacact gaccatttct tttctgatgg aattgccact
    18361 gtgtacgtgt ggggcaggcg aaacgattaa tttattcata tttttaattt ttctgtctta
    18421 tgatttttgt gttttcctgt ttcgaatcat cgttccgacg gtagatttcc cataacgaat
    18481 gtaatcgacc aaaggtctgt tggaagggca aataaaactg catgaaccac attctataca
    18541 atccattaca cgattttctt ccatattttc ccacatctgt ttttctccct gattcataag
    18601 aagatacggt tctaagccca tcgggcagtg aaaaacacat ttagcacagc gaatgcaatt
    18661 aaatactttt cctcttttcg attccgagga agggataaac aatatggaag atgtcccttt
    18721 agccaccgac aggtcgagac ttggcaatgc acgtcccatc atagggcctc cggctacaac
    18781 ttttccagta tcctgaggca ttcctcccgt ttcttctata atatgtgtaa agggagttcc
    18841 tatacgaacc caatagttac ctggttcggg tactgatttg ccagttaccg taatgattcg
    18901 ctcaataagc ggtttatttt tttggacggc ttcatacacg gcaaaagttg tggctacgtt
    18961 ttgaactact gcgccgactt caataggtaa agctccactg ggaacttctc gtcccgttaa
    19021 tgcctgaatc aattgttttt cacttccctg tgggtacttg actttgagag gaactacttc
    19081 tatgccttcc attgttttcg cccatttttc caagttgagc acagcatcca ttttattgct
    19141 ttcgattcca ataattgctt tttttactcg aattgctttc atgagtatgc tgatgccgat
    19201 gataatctct tctccttttt ccagcattag ctgatggtcg caagtgagat aaggttcaca
    19261 ttctgctccg ttgataataa gtacttcagc ttttttcccc tgaggaatag ataattttac
    19321 gtgcgttggg aaagttgctc ctcctaatcc aactattccg gcttctgcaa cttttttaat
    19381 tatttcgtga ggctcaaggt tacatactct ttttacttca ggcgacaaat caacttcatc
    19441 gagccaatcg tcacctatta catcaataaa tactgccgga aatttatatc cgaatgcatc
    19501 cattgcatta tcaatttttg atacttttcc cgatactgag gaatgaacat tggcagaaat
    19561 aaatccattt gctcgtccaa tcaattgacc aacttttact atttcacctc tggatacttc
    19621 tgctacgctc ggattgcctg aataatgacg aagtggtata atcacctgct ggggcaacga
    19681 gattattttt gttttttttc cagctgaaaa tttattttcc ggggggtgga caccgcccat
    19741 tttaaatgtc ttcattaaaa gtttatgatt ttttcttatc tatttaaatc aaataaaaat
    19801 tgtgttgaaa aaacttattt ctcagttcct gttgtagaag gttcctctat ttttgtttta
    19861 cgtggtggaa aattcaactc cacgatagca ttagttgggc aaacttcaac gcatttgcgg
    19921 cataatttac acttttcgga atcgatataa gccaaattgt tccccatttc aatggcttcg
    19981 taggggcaca cgttgaagca tttggagcag ccaatacaag ccactttgca aactttttta
    20041 tttacagctc ccttatcctt gtttacgcaa ttcacgtaaa ttcttctcga tttaggacct
    20101 tttcgtctta gttcaatgat gtttttggga catgctttta cgcaggcgcc acaggctgtg
    20161 catttatctt catcaacttc gggcaacatt gtttcgggat taatatggat agcatcgaaa
    20221 gtgcaagaaa cttcgcaatc tcccagtccc aaacaacccc aggaacaacc ggtttcacct
    20281 ccatataaat tgtgtacaat cgtgcatgat tttacaccat cgtaactatt ggttttttca
    20341 cgcacttcgc aagttcctcc gcagcgaacc acagctatgg taggaactac tgccgctgct
    20401 ttctttccca aaatatcggc cactttttcc atagtagccg tacctcctac cggacaagat
    20461 aaattatcta gtgtatcggc tgttacgcat gctgctgcaa atgcccggca accagggaac
    20521 ccgcaaccac cacagttggc agccggtaac accgcttcca cttcgtcgat gcgtgcatct
    20581 tcttctacat gaaatttttg ggcaataaaa tatagaataa ctgccgataa agcgccgatt
    20641 cctccaagtg aaactactga tgctaatatt aaattcattg ctgatttatt tgtgatttga
    20701 ttttactaaa gaatttttta ggtaagtgat tcttttttga cgtgaaattc aaattttctt
    20761 tttaatttat ggttgaataa tgaaagtatc ccataatatg gaaatatgga gcacagggct
    20821 atgatgccgg aaattgtttc gttttgctga aaataattgg aaattattaa ggtaagaaca
    20881 atcagcaaca acgggaaaat aaaagccagc aaaacggctt gaagtcccat tgaatttcgt
    20941 ccaaaaatta ctacttgatc accttctttt attgaagaat cggttacgac tacatcaaca
    21001 attttttttt cctcttccat tacagtacag gcacttttgg catgacacgc actgcatgcc
    21061 gactgctgga tgataagtat ttgagcttta ctcccttcaa tatgttgaac tatgccgatg
    21121 tgttctattt gttgcattca attttttttt tgcaatgttg acattacaat ttgtcggcaa
    21181 aaatacggat tatttatatt tacatcaaaa aaaatcaata aaaaatcgat tttgtagagt
    21241 caaccgggaa tttgagaagt taaatttttt aaaagtatga gtcccctctt aaatttgatt
    21301 ttttcaaaaa agggaaaaat aagattattt gaaaagcagc attaaatttt tttgttgttt
    21361 cgatatttta caataatttg caatataaaa gttatttttg tt
//
LOCUS       DAOSIB010000031        20759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_2820_length_20759_cov_12.122440, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000031 DAOSIB010000000
VERSION     DAOSIB010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 20759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20759
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2820_length_20759_cov_12.122440"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..6165
                     /locus_tag="PLD44_04390"
     CDS             277..6165
                     /locus_tag="PLD44_04390"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOF98606.1"
                     /translation="MKKLSFILLAVVFVVVAFAAFQQPTVSFDERWKKVEELAEKQLP
                     ESALKEVDAILKDAKKQNNSPEIIKATLYKMRFTLEKDPDQAPQLIKEFETFATSTSD
                     TAEKSLLYSMTAELYAQYFQQKAYSIRQRTEISDYVPENIDEWTKNIFFDKVSQLLAA
                     SLEPAEMLQKTDIHKFETILEKGDSSHRVQPTLFDFLAYRRIDILQSLEGAQSIKNPL
                     KSEDLFSDLSHFVSLLLDTTYSKSVENQVLQTYQQLLSFHLKDNNVPALLYADLQRLT
                     YLKENSQLSNSDELYLSALARLSEQYANNENVVEVMAAMAQYYLDKEYELEENMNFAK
                     KAYAICSEGIRRFPDYPRIALLKNIQQQITQKSVSLRHSDVVRSKSQLSLYISTKNVE
                     DLQMQIYRVNASSLDYFSFTKNPRNSGKDFPDRTLWQTSEIKIKPDTNFVAVDTVLKI
                     PSGTFGIYEVQLMIKGDTSSEVARSSYVVSDLSFMVRQNEPKKTALYVLNSETGWQQK
                     EVKVSLYEQKWTGNFYQMNLLQTAVTDKNGFCTFPYNNQGTKWIFLEKGEDRYFSSSV
                     NTYYWERDTNKEKPVQLEFFTDRAVYRPGQTVYFKGIAYYADAKRQEVAKSLKFDVSL
                     IDTHYQKIASKTFTTNKFGSFAGEFVLPADGLNGTFRLESGNFSKTIWVEEYKRPTFE
                     VKIQKPTDEVKFGEKVTLTGNVKAYSGYNIADAKVNFRVVRRPHWYCSWIRSEEKAIS
                     NGETKTDANGNFTLFFIPEKSKENFSFRKDAYYVYTVYAEVTDTKGETQQGQQFVAVG
                     DKSLFILADVPEKWNKKEKADFPLRLETINGEKVDGTVQFTVYRFENSEDFATNEKWA
                     EMEEEGEEEISMKSAKKIMEGTFNTKDGKLKLDVNKWESGGYRIVFSTLDQSGKKVEL
                     GKNFILYGKNDKRPSIQTMTWLPVEEITCEVGETAEVVFGSSADNVAVLYEMMQGNTV
                     LESKWIRMNNEIKTFPIPFKAEYGDGITVMFTFMKKGLFFTESVIIRRKIAEKKLTPT
                     LTVFRNKLLPGEKAEWTVNIPEIKQIKKQAELLMDMYDASLDVIRPNEWTFQPIFRGN
                     FMESPRWDAPYNRIISDNIDIPVKYIPVEEYRLDRLNWFGLDLMAGQKYNGPFVVRGL
                     KSLSATSEFREISAMNEKFEEETVVAFSVVKTVKTDLTNVIMQPETSPVIRKNFNETA
                     FFYPQLYTDSAGDVKFSFTAPESLTRWNIKMLAHTPDLYFGQADAQAITQQDLMIQMN
                     LPRFVRCSDTLILSCIITNLSEKEISPVAHFELLDAANEQPVAVFEDSKVQLSPNETK
                     TVRWQLKDFSSHELLICKVMATAGSFSDGEQNYLPVLPDKILVTESLPMTVRGHQTKT
                     FQFESLQKKFSQVETKNLTLEFAAQPVWYAIQALPTLAEPENQNAVDYFAAYYVSTLA
                     AYIVQSYPEIEKIFRRWEKSETGKNALLSNLEKNSELKNFLLKETPWLMAAQDEMEQK
                     NRLALLFDLNMQQDKSQQYLDKLLKLQLSTGAFTWFEGMTENRYITQLVLLNMKRLHE
                     KTGLPMDKRLESAMTQALNYLDKEIVNDFEQLKKNRRNFEKENCISPLQLQYLYLRSS
                     YSEIPVPDFSQEAVKFYRHQAEKFWTDFSLYGKSQAAIVAYRDGKMALARNILKSLKE
                     NALKSEEMGMYWAKNKAGYFWNERPVAVQTAIMEAFSEIGADTSDLEEMKIWLLKQKQ
                     TQRWDSPISTVDAVFALLTIGNNWLQNNDKVIIKIGDKELQPSSTEAGTGYFKQSLPV
                     EQITSASSRITVSKNSDGIGWGAMYWQYLQKMDQVQSTVGALNISKKLFVEKVTSAGR
                     TLVPIEQVKISLGDKVVTRLVVTTDRNLEFVALKDIRAAGLEPVNQISGCQWKQGVCY
                     YQTIKDASAEFFFSSLPKGTYVFEYALWANNVGTFNGGTASIQCQYAPEFVGYAAGQQ
                     VVVQ"
     gene            complement(6231..7616)
                     /locus_tag="PLD44_04395"
     CDS             complement(6231..7616)
                     /locus_tag="PLD44_04395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF98607.1"
                     /translation="MKIQINIKLKENRWLLMAIVIGSFFNSFMSSSLNIAMPVIDNEY
                     HVGLAKVAWISESFLLASAVGLMLAGWLADQFGRRKTFLFGNLIFGFISLFIYFVPGF
                     YPAVMLRFIQGLGSALIMASAPALIASAFDPSRRGFVLGIQTTFTYVGLSLAPLLGGA
                     LTQIFGWRSLFLFNFIFGLAALSIMFFGVKREWKAETRNKFDVGGYVIFLLAIVALVL
                     ALDWMNKFSIIMLLFSLMAGYVFIRYELKKEMPFISIAFLKSNKYFTNSNFAALINYA
                     TTFAISYVLSLYLQNVRGMTPVGAGLILCFQPLMMALFSAYAGHLSDRHNPGKIASYG
                     MAMIALALFIIGITLSEKLPLAIIAGLLAFLGFGFALFSSPNTNAVMSSADAGSYGFA
                     SAFLAFNRLFGQFISMALASAFILLSNHTRLATVEEQALLMASKIIFIFFGLLSCLGV
                     YLSYQRIRMKK"
     gene            7928..10393
                     /locus_tag="PLD44_04400"
     CDS             7928..10393
                     /locus_tag="PLD44_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOF98608.1"
                     /translation="MIYPKHFEQKIDFITIRQLLKDKCVSTLGIEKVDEIRFSSNFDE
                     IFHWLSETNEMLKVLTTEVQELPVNHFYDLRSSLSKIRIEGLYLTETEIFQLYQTLEN
                     VLTITNYISQLNPQLYPCLHQLTGNVNVFKPIIQRVDAILSKFGKIKDQASPQLTQIR
                     KNMTEVQNNISKSIQEILRKAQTNGYVEKDTTPALREGRLVIPVPQAFKRKINGIIHD
                     ESASGKTVYVEPAEIVEMNNRLRELEREERRETIRILTLFTDFVRPYLPDIQESQNFL
                     GKIDFLRAKALLAIDIHAIQPQLENHCLIDWHKAIHPLLFLSFSKQNKQVVPLDIQLN
                     EKQRILIISGPNAGGKSVCLKTTALLQYMLQCGLLIPLHESSRTGIFEHLFIDIGDEQ
                     SIEDDLSTYSSHLLNMKFFIRYSNDKTLLLIDEFGSGTEPQLGGAIAEAVLDRINRNK
                     AFGVITTHYTNLKQFAEETEGIVNGAMLFDRQKLQPLFQLQIGMPGSSFAIEIARKIG
                     LPEDIIDQASKKIGKDYLNYDRYVQDIIRDKRYWQNKREQIKLKEKQMEETIRRYETE
                     LTELKRQRTEIIQKAKEEAQNILEETNAKIENTIRQIKEAQAEKEKTKEIRKELENFK
                     QSVNQSIDNRFSVNPAAVSTKKKLVKKVTPKEEIHKKDVQEEKISVGSNVRLKNQGTT
                     GTVLEINGNEAIVAFGQMKSTVPFSKLEPLSRTKLKKENQNKKNIHVNTIEAVRQRKL
                     EFKTEIDVRGMRSNEALQAITYYIDDAIMVGVPSVRILHGTGTGALRQIIREYLRTIP
                     AVRNFHDEHIQFGGSGITVVELE"
     gene            10601..10762
                     /locus_tag="PLD44_04405"
     CDS             10601..10762
                     /locus_tag="PLD44_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98609.1"
                     /translation="MKKVLLFCAVIFAFAACAPKESKEASTTEDSVVVVDTVVVDSMV
                     VDTTAAPMK"
     gene            complement(10838..11791)
                     /locus_tag="PLD44_04410"
     CDS             complement(10838..11791)
                     /locus_tag="PLD44_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOF98610.1"
                     /translation="MEKPYVIGIDMGGTNTVFGIVDVRGNVISKSAIKTAVHDDVNLY
                     VNDIYHELMKLIDAEGGIDQIRGIGVGAPNGNYYSGNIEYAPNLPWKGVIPFAKLLNE
                     KFGVPVALTNDANAAAIGEMAYGIARGMKDFIMITLGTGVGSGIVIDGKLVYGHDGFA
                     GELGHTTSIRNGRLCGCGKNGCLEAYSSATGVARTAREILETTNQDSILRNIPLADIT
                     SKDVYDAAKEGDQVAIDIFNFTGKILGEAFADFVAFSSPEAIILFGGLSKAGEFILNP
                     IIENMEKNLLPIWKGKIKILFSELKESDAAVLGASALAWEL"
     gene            complement(11814..13169)
                     /locus_tag="PLD44_04415"
     CDS             complement(11814..13169)
                     /locus_tag="PLD44_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007411075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF98611.1"
                     /translation="MNAEKKSGNLVAIITMFFLFAMISFVTNLAAPIGVIWKNQPAFE
                     GSNFLGMLGNMMNFLAYLFMGIPAGKLLSKIGYKKTALVAIAVGFLGVLTQYLSGIIG
                     VGAELFSLPANFFVYLLGAFIAGFSVCMLNTVVNPMLNLLGGGGNKGNQLIQIGATFN
                     SLAATITPMFVGALIGDITKERILSGEVTINNVNPVLYIALGVFALAFLILLFIPIAD
                     PDENRKKRSEIKYEHSPWNFRHFVLGSIAIFIYVGVEVGIPGTLNFFLADTTIKGASL
                     DPQKAAAIAGFVAGTYWFLMLIGRFLGSLVGGKVSSKTMLTFVSALGIALVLLAILLP
                     KDITTSMPVFTGSSFSMVQVPLIALFLVLCGLCTSIMWGSIFNLAVEGLGKYTAAASG
                     IFMMMVVGGGILPLLQNWIADISTYQASYIVILVGLLYILFYALIGSKNVNKDIPVEV
                     E"
     gene            complement(13357..15525)
                     /locus_tag="PLD44_04420"
     CDS             complement(13357..15525)
                     /locus_tag="PLD44_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOF98612.1"
                     /translation="MKRIFLAFVLAFAVQFSFADLLDDIVDGKFNVNNIMVPRSMKDG
                     ENYTLMKDNAVLKYNYKTGQLVDTIFSIHRLKDAPFKEINGYEFSPNEAKLLVYNNVK
                     KRYRRTFTADYYVYDIKRNEIQPLSDNGSQEVPLFSPDSRYIAFGRNNNLYLKKLDFN
                     TESAITKDGEPGKIINGLPDWLYEEEFEATRYFVWSPDSKLLAFVKFDETEVPQFSFQ
                     LYENKNSMDDLMLYPSLVSFKYPKAGQNISKVSVCVYEERTKAIRTVQLSNVGEDFYI
                     PRIKWTNSLDQLAVFVLNRNQNQLKMFLANPKSTLSTLILQENDKYYIDYQNIDACQF
                     TSDNQYFIFLSEKDGFRHVYQHRINGTPVKQLTKGNFDVTQVYGFDEKNQTLYYQSAE
                     SSPLQRDIYSVSLKGVKKKLTNGNGFHNAIFNSTFSFFVDNFSNLQTPDKLILYSNNG
                     NFIREMQNSDGILENWNTLHLPKKEFFSFTTSENISLNGWMLKPLNFDPNKKYPLLLI
                     QYSGPNSQMVLDKWDVDWEYYLAENNMLVVCVDGRGTGARGVEFRKCTYEQLGVLEAK
                     DQTEAARFFSKQDYIDPQRIGIWGWSYGGFLTLMAMSSGENVFKAGIAVAPVTDWRFY
                     NAAYTERYMRRPQENFKGYDLASPIMKADRLKGELLIIHGTADDNVHAQNTLMYVKRL
                     VEADKQYNMQLFTDKNHSILGKQTRRYLYRSMSNFLFKNL"
     gene            15691..16512
                     /locus_tag="PLD44_04425"
     CDS             15691..16512
                     /locus_tag="PLD44_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOF98613.1"
                     /translation="MNDDNITNHEQNINKTGICLSGGGALGFAHIGVLQALEENGIFP
                     VQIAGSSMGAIVGTLYAAGFTPAQMLQMIREDKLYKITKLMSIHPMSLKSGLSTHKML
                     RDLIKELIPHNSFELLKKKMSVCVSNLNKAEWEIIESGKELDKWVAASASIPGVFEAI
                     QVKKEYYVDGGLLNNMPAQALKENCSTIIGVDVIPHLNEVQLKKPIDTLTYSIRIIQH
                     KNAQKGRELCQYLIEPKAIRKFHEFSFDAYQQIYEEGYQCTHTFIENNPAINNGI"
     gene            complement(16593..18167)
                     /locus_tag="PLD44_04430"
     CDS             complement(16593..18167)
                     /locus_tag="PLD44_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOF98614.1"
                     /translation="MNSELVVDVKPSEISIALLEDKRLVELAQETREEQFAVGNIYLG
                     KVKKIMPGLNAAFVDVGYEKDAFIHYLDLGPNFNTLSQFTLQALSDPKKAPSMQKVKM
                     LPEIEKSGFINDLLKPGQQILVQVAKEPISTKGPRLTAEISLAGRFLVLIPFNDKVLI
                     SQKIKSENERVRLKQLIQSIKPKGFGVIVRTVAEDKKVAELINELNVLVKRWDEAVSK
                     LQQAKGVTLISEELGRTVGIIRDLFNPSFKNIYVNDPNVFAEVQDYIEWIAPEQKNIV
                     KLYKDETPIFDHFGITKQIKSSFGKTVSFKNGAYLIIEHTEALHVIDVNSGNRAKTGN
                     GQEANALEVNLAAADEIARQLRLRDMGGIIVVDFIDLHESDNRQKVYDRMKEMMANDR
                     AKHNILPLSKFGLMQITRQRVRPDTHVDIEEACPTCFGTGKIKPSILFTDLLESKIDY
                     LVNTLKVKEFTLYVHPYVEAYISKGMIPLKNKWKIKFGRGCKVRASQELGFLQYKFID
                     NKGMELNLQEEIEKIS"
     gene            complement(18402..18674)
                     /locus_tag="PLD44_04435"
     CDS             complement(18402..18674)
                     /locus_tag="PLD44_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOF98615.1"
                     /translation="MTKAEIVNEIAKKTGIDKATVLASVEAFMDAIKDSLSKNENVYL
                     RGFGSFVVKTRAQKTARNISKNTTIIIPPHNIPSFKPAKKFMSVVK"
     gene            19114..19776
                     /gene="ung"
                     /locus_tag="PLD44_04440"
     CDS             19114..19776
                     /gene="ung"
                     /locus_tag="PLD44_04440"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOF98616.1"
                     /translation="MDVKIESSWKEVLQSEFEKEYFKQLTDFVRKEYKTKKIFPPGSL
                     IFNAFNLCPYDKVKVVIIGQDPYHGPGQANGLCFSVNEGVDFPPSLVNIYKEIENEFA
                     KPMPKNGDLSSWAKQGVLLLNAILTVQAHLAGSHQGKGWETFTDAVIRILAEKKEHLV
                     FLLWGSYAQQKGLYIDTNKHLVLKSPHPSPLSAYRGFIGNRHFQLANQYLKEHGMSEI
                     EW"
     gene            19974..20612
                     /locus_tag="PLD44_04445"
     CDS             19974..20612
                     /locus_tag="PLD44_04445"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOF98617.1"
                     /translation="MTVIHFFVLKYSTVEFIGLFISLFGLFIRAYAVGHTPANTSGRN
                     TKEQLADELNTTGIYSMVRHPLYLGNFFMWLGAAVLTKNGWFIVAFILLYWIYYERIM
                     FAEEQFLRRKFGSVYTDWANKTPAFIPSFKKYIPSKYPFSWKKVLKKEKNGLLAVFVL
                     LFVFHNINYSFLTNHIRIDLNWIAWGTFAAGILYLVLKFLKKKTSILNETGR"
BASE COUNT         6771 a         3973 c         3886 g         6129 t
ORIGIN      
        1 aatatatgaa ttttcaaaaa caacattttt aaaaactttc gtacggcagt gataattttc
       61 catttctcaa ccataaggtc gctcctacag agctttcaag tcacacaggg acaacctttt
      121 ggtagaactg agtagagccg ttccatgcaa catctctaca aaaacaattt tccctgtcga
      181 attttgctca aaaaatttct tcctgccgat tttggtacta tatttgtctt ccatttcgct
      241 tcacaaataa aaatatccgt ttaaaatttc ttcctcatga aaaagctttc ttttatattg
      301 cttgccgtag tttttgttgt ggtggctttt gctgcctttc agcaaccgac tgtttccttt
      361 gacgaacgct ggaaaaaagt ggaagaactg gccgagaaac agttgcccga atcggcactg
      421 aaagaagtgg atgccatttt gaaagacgca aaaaaacaaa acaattctcc ggaaattatc
      481 aaagctactc tttacaaaat gcgtttcacg ctggaaaaag atcccgatca ggcaccacaa
      541 ctcatcaaag aatttgaaac ttttgcaacc agtacttccg acacagccga aaaatcgttg
      601 ctttattcta tgacggcaga gctctatgct caatactttc agcaaaaagc ctattccatt
      661 cgtcaacgaa cggaaatttc cgattatgtt ccggagaata tcgacgaatg gaccaaaaat
      721 attttcttcg ataaagtcag tcagttgctg gctgcttcat tggaaccggc agaaatgctt
      781 caaaaaaccg atattcataa atttgaaacc attctcgaga aaggagatag cagccatcgg
      841 gtacagccca cactttttga ttttttggcc tatcgccgta tcgacatttt gcaatcgttg
      901 gaaggtgcac aatccataaa aaatccgtta aaatccgaag acctgttttc tgacctatct
      961 cattttgttt cgttattatt ggatacaacc tattccaaat ccgttgaaaa tcaggtgttg
     1021 caaacctatc agcaattgct ttcttttcac ttgaaggata ataatgtgcc ggcacttttg
     1081 tatgccgatc ttcagcggtt aacatatctc aaagaaaatt cccagttgtc gaatagcgat
     1141 gagctgtatt tgtcggctct ggcacgttta tcggaacaat atgcaaacaa cgaaaatgta
     1201 gtggaagtta tggctgcaat ggcacagtat tatctcgaca aagaatatga attggaagaa
     1261 aatatgaatt ttgcgaaaaa ggcttacgct atttgttccg aaggaattcg ccgttttcct
     1321 gattatccgc gtattgcatt gttgaaaaat atccagcagc aaataacgca gaagtctgtt
     1381 tctttgcggc attctgatgt tgttcgatca aaatcacaac tctcattgta tatttctaca
     1441 aaaaacgtgg aagatttaca aatgcagatt taccgcgtca atgcttcatc gctcgattat
     1501 ttttcattta ccaaaaatcc gcgaaattca ggaaaagatt ttcctgaccg taccttgtgg
     1561 caaacttcgg aaataaaaat taagccggat acaaattttg tagccgtcga tactgtttta
     1621 aaaattcctt cgggaacttt tggaatttac gaagttcagt tgatgataaa aggcgatact
     1681 tccagtgaag ttgccagaag ttcttatgtg gtttccgatt taagttttat ggtacgccag
     1741 aacgagccga aaaagacagc attatatgtt ttaaatagcg aaaccggatg gcaacaaaaa
     1801 gaagtgaaag tgagccttta cgaacaaaaa tggacaggca atttttatca gatgaacctt
     1861 ttacaaactg ctgttaccga caaaaacggt ttttgcacat ttccttacaa caatcagggt
     1921 acgaaatgga tatttctcga aaaaggagaa gatcgatatt ttagttcttc tgtaaatact
     1981 tattattggg aaagagatac aaataaagaa aaaccggttc agttggaatt tttcaccgac
     2041 cgtgcagtgt atcgtcccgg acaaacagtc tattttaaag gaattgccta ttatgccgat
     2101 gcgaagcgtc aggaagtggc aaaatcgcta aaatttgatg tttcgcttat tgatacccat
     2161 tatcagaaaa ttgcttcaaa aacattcact accaataaat ttggttcatt tgcaggagaa
     2221 tttgtcttgc ctgccgatgg attgaatgga actttccggc ttgagtcggg aaatttttcc
     2281 aaaactattt gggtggagga atacaaacgt cccacttttg aagtaaaaat tcaaaaaccg
     2341 accgatgaag taaagtttgg ggaaaaagtt acgctaaccg gcaatgtgaa ggcttattca
     2401 ggttataata ttgcggatgc aaaagtaaat tttcgtgtag ttcgacgtcc acactggtat
     2461 tgcagttgga tcagaagcga agaaaaagca atttcgaatg gagaaacaaa gactgatgca
     2521 aacggtaatt ttacactttt ttttattccc gaaaaatcaa aggaaaactt ttctttcaga
     2581 aaagatgcct attatgttta cacagtttat gccgaagtta ccgataccaa aggcgaaaca
     2641 caacaaggtc agcagtttgt agcggttggc gacaagtcgt tgtttatttt ggccgatgtt
     2701 cccgaaaaat ggaacaagaa agaaaaagcc gattttccgc tgcggcttga aaccataaac
     2761 ggtgaaaagg tggatggtac ggtacaattt accgtttatc gatttgaaaa ttcggaagat
     2821 tttgcaacca atgaaaaatg ggcagaaatg gaagaagaag gtgaagaaga gatttccatg
     2881 aagtcagcaa aaaagataat ggaaggaact ttcaatacca aagatggtaa gttaaaatta
     2941 gatgtaaata aatgggaatc gggtggttat cgtatagttt tttctacgct tgatcagtcg
     3001 ggcaaaaaag tggaattggg taaaaacttt attctttacg gaaaaaacga caaacgtccg
     3061 tctattcaaa ccatgacctg gttgcctgtg gaagaaataa cctgtgaagt aggtgaaaca
     3121 gctgaagttg tttttggttc gtcggctgat aatgttgccg tgctttatga aatgatgcag
     3181 ggaaataccg ttttggaaag caaatggatc aggatgaaca acgaaatcaa aacttttccg
     3241 attccattca aagctgaata tggagatggc atcacggtga tgtttacctt tatgaaaaaa
     3301 ggactgtttt tcactgaaag tgtaatcatt cgccgaaaga tagcggagaa gaagttgacg
     3361 ccaactttaa ccgtttttcg caataaactt ttgccgggtg aaaaagcgga atggacagtc
     3421 aatattcctg aaataaaaca gattaaaaaa caggcagaat tgttgatgga catgtacgat
     3481 gcttcgctgg atgtcattcg gccgaatgaa tggacatttc agcctatctt tcgtggaaat
     3541 tttatggaaa gtccgcgctg ggatgcacct tacaacagga taatttccga taatattgat
     3601 attccggtta aatatattcc ggtagaagaa tatcggttgg atcggttgaa ctggtttggt
     3661 ctggacttga tggcaggtca aaagtataat ggtccttttg ttgtaagagg cttaaaaagt
     3721 ttaagtgcaa catctgaatt tagagagatt tcggccatga atgagaaatt cgaggaagag
     3781 actgttgttg ctttttcagt agtaaaaaca gtaaaaaccg atttgacgaa tgtaatcatg
     3841 caacctgaaa cttctccagt cattcggaaa aatttcaatg aaacggcatt tttctatccg
     3901 caactttata ccgacagtgc aggcgatgtg aaattttctt tcactgcacc ggaaagtttg
     3961 acccgatgga acattaaaat gctggctcat acgcccgatt tatatttcgg acaagctgat
     4021 gcacaggcca tcactcagca agatttaatg attcagatga atttgcctcg ttttgttcgt
     4081 tgctcggaca cgttgatatt gagttgcatt attaccaacc tttcggaaaa agaaatttcc
     4141 ccggtggcac atttcgaact gttggatgca gcaaatgaac agccggttgc agtttttgaa
     4201 gacagcaaag tacagttatc ccccaacgaa acaaaaaccg tgcggtggca attaaaagat
     4261 ttttcttcgc atgaactgtt gatttgtaag gtgatggcaa cagcaggcag tttcagcgat
     4321 ggagagcaga attatcttcc ggttttgccc gataagattt tggtaacaga aagtttaccc
     4381 atgacagtac gcggtcatca aaccaaaact tttcaatttg aaagtttaca gaaaaaattc
     4441 tcacaggtgg agactaaaaa tctgacgctg gaatttgctg ctcaaccggt ttggtacgcc
     4501 attcaggctt tgcctactct tgccgagccc gaaaaccaaa acgctgttga ttattttgct
     4561 gcctattatg tttctacgct ggcagcctat attgttcaat cttatcccga aattgaaaag
     4621 atatttcgac gttgggaaaa gtcggaaaca gggaaaaatg cacttttatc gaatctggag
     4681 aaaaattccg aactgaaaaa ctttttactg aaggaaacac cctggttgat ggctgctcag
     4741 gatgaaatgg agcagaaaaa tcgattggct ttacttttcg atttgaatat gcagcaagac
     4801 aaatcgcaac aatatctcga caaattgttg aaacttcaac tttcgaccgg agctttcact
     4861 tggtttgaag gcatgacgga gaatcgttac atcacgcagc ttgttttgct caatatgaaa
     4921 aggttacacg aaaaaaccgg tttgccaatg gataaaaggt tggagtcggc catgacgcag
     4981 gctttgaatt atcttgacaa agaaattgtc aatgattttg agcaactgaa gaaaaaccgg
     5041 agaaattttg aaaaggaaaa ctgtatcagt ccgcttcaac ttcagtattt gtatctccgt
     5101 tcgagctatt cggaaattcc cgttcccgat ttttcacagg aagcggtaaa attttatcgt
     5161 catcaggcgg aaaaattctg gacggatttc tcactttatg gcaaatcgca ggctgccatt
     5221 gttgcttatc gtgatggaaa aatggctttg gctcgcaaca tactgaaatc tttaaaagaa
     5281 aatgcactca aatcggaaga aatgggcatg tattgggcta aaaacaaagc cggatatttt
     5341 tggaacgaac gtcctgttgc tgttcaaaca gctattatgg aagctttctc ggaaattgga
     5401 gctgacactt ccgacctgga agaaatgaaa atctggttgc tcaaacaaaa acaaacgcaa
     5461 cgatgggatt cgcccatttc aacagttgat gctgtttttg ccctgcttac cataggaaat
     5521 aactggttgc agaacaacga taaagttatt atcaaaattg gcgataaaga acttcagcct
     5581 tcatctaccg aagccgggac cggatatttc aagcaatcgt tgcctgtgga gcaaataaca
     5641 tctgcttcaa gtcgcatcac ggtcagcaaa aactcggatg gaattggttg gggagccatg
     5701 tattggcaat atttacaaaa aatggatcag gtgcagagta cggtcggtgc attgaatatt
     5761 tccaaaaaat tgtttgtgga aaaagttact tccgctggtc gaacgcttgt tccaatcgaa
     5821 caggttaaga tttcgctggg agacaaggtt gttacgcggc tggtggttac taccgacaga
     5881 aatctcgaat ttgttgcttt gaaggacatt cgtgcggcgg gtctcgaacc ggtgaatcaa
     5941 atttcgggct gtcagtggaa acagggtgtt tgttattatc agaccataaa agatgcttcg
     6001 gcagaatttt tcttttcgtc tttgccaaaa ggaacctatg tgtttgaata tgccctttgg
     6061 gctaataacg tcggaacttt caacggcgga acagcttcca ttcagtgtca gtatgcaccg
     6121 gaatttgtcg gctatgcagc aggacaacag gttgttgttc aataactttt cgggaaaaat
     6181 ttgatgattt gagtgtgtaa agaaattcgc atgattttgt tttggtattt ctattttttc
     6241 atgcgaattc tctgataaga aagataaact cccagacaac taagcaagcc aaaaaagatg
     6301 aaaattattt ttgaggccat tagtaaagcc tgttcctcaa cggttgctaa gcgggtatga
     6361 ttactcaaca aaataaatgc cgatgccaat gccattgaaa tgaactgtcc gaacagccgg
     6421 ttgaaagcca aaaaagccga agcaaaacca taagaacccg catctgccga gctcatcacg
     6481 gcattggtgt tgggtgaaga aaaaagagca aatccaaatc ccaaaaatgc caacaatccg
     6541 gcgataattg ccagcggaag tttttcgctc agagtaattc caatgataaa caatgccaaa
     6601 gcgatcatag ccatcccgta agatgctatt ttgccgggat tgtgtctgtc cgacaaatga
     6661 cctgcatagg ctgaaaacaa agccatcatc agtggttgaa agcataaaat aagaccggcg
     6721 ccgacaggtg tcattccgcg tacattttgc aggtacaaac tcagcacata actaatggca
     6781 aatgtggtgg cgtaattgat taaagctgcg aaattggaat tggtaaaata tttgttcgat
     6841 ttcagaaaag cgattgaaat aaatggcatt tctttcttca gttcatagcg aataaaaaca
     6901 taacctgcca taagactgaa taacagcatg ataattgaaa atttgttcat ccagtcaagt
     6961 gcaagtacca atgcaactat ggccaagaga aaaataacat atccgccaac atcaaatttg
     7021 tttcttgttt cggctttcca ttctcttttc acaccaaaaa acataatgga caaagccgcc
     7081 agtccaaaaa taaagttgaa aagaaacaga cttctccacc cgaaaatttg agtcaaagcg
     7141 ccacccagaa gcggagccag tgaaagtccg acgtaagtaa aggtggtttg aattcccagt
     7201 acaaatcctc ttctggaagg gtcgaaagca gaagcaatca gggcaggagc ggaggccatt
     7261 atcaatgcgc tccccaatcc ttgtataaat cgcaacatta cagccgggta aaaaccggga
     7321 acaaagtaaa tgaaaagtga aataaaaccg aaaatcaaat ttccaaaaag aaaagttttg
     7381 cgtcgcccaa actgatcggc cagccaaccg gccaacatta atccgacggc agaagccagc
     7441 agaaaagatt ccgatatcca tgctactttg gccagcccta catggtattc gttgtcgata
     7501 acgggcatgg cgatattcag cgatgaggac atgaaactgt taaaaaatga acctatcaca
     7561 atggccatca ataaccatcg gttttctttc aattttatat tgatttgtat tttcataccg
     7621 tttgcttata caatttacta aaatactttt taaaacggct taatgaaata tttttcataa
     7681 ttttgtcttc ccacagaggg gttcagtctg caaacaacat aatatgaaaa atcaatcatt
     7741 cggtttattt actttacaaa agtaaggtga taaacatggt tggtttaaag aaaaaggcaa
     7801 gcaaatttat aatatttttt ggacaaaact gtttttaaaa aaatagagtt tatcttatct
     7861 ttctcaatac gcagcaaacc aagtttttaa tagctatttg attttaaaaa atttaattac
     7921 atgctcaatg atttatccta agcacttcga gcagaaaatc gacttcatta ccattcggca
     7981 actgctgaaa gataaatgtg tcagcacgtt gggaatcgaa aaagttgatg aaattcgttt
     8041 ttcttccaac tttgatgaaa tctttcattg gctgtcggaa accaatgaaa tgttgaaagt
     8101 gttgaccact gaagtgcaag aattgcctgt aaatcatttt tacgatttgc gttcttctct
     8161 ttccaaaatt cgtatcgaag gtctttatct taccgaaaca gaaatatttc agttgtatca
     8221 gacgctggaa aacgtgctga ccatcaccaa ttatatttca caactgaatc cgcagcttta
     8281 tccttgtctt catcaactga ccggcaacgt aaatgttttc aaacccatca tacaacgtgt
     8341 cgacgccatt ctttccaaat ttggaaaaat caaggatcag gcatctcctc agttgacgca
     8401 gatacgcaaa aacatgaccg aagttcagaa caatatctca aaatctattc aggaaatttt
     8461 gcgtaaagcg cagaccaacg ggtatgtaga gaaagacacc actcccgctc tacgtgaagg
     8521 gaggttggta atacctgtac cacaggcatt taaaagaaaa atcaacggca ttatccacga
     8581 tgaatcggca agcggaaaaa ccgtttatgt ggaaccggcc gaaatagtgg agatgaataa
     8641 ccggttgcgt gaactcgaaa gagaagaacg ccgcgaaaca atccgaattt tgaccctatt
     8701 taccgatttt gtacgtccct atttgcctga cattcaagaa tcacagaatt ttttgggaaa
     8761 aatagatttt ttgcgtgcca aagcattact ggctattgat atccatgcca tacaaccgca
     8821 attggaaaat cattgcctca tcgactggca taaagccatt catcccctgc tttttctttc
     8881 attctccaaa caaaacaaac aagttgttcc gctggatatt caactgaatg agaaacagcg
     8941 tattctaatt atttcaggtc ccaatgcagg aggaaaatcc gtttgtctga aaactacagc
     9001 gttgttgcaa tacatgctgc aatgcggctt gctgatcccg ctacatgaaa gcagtagaac
     9061 tggaattttt gaacatcttt ttatcgatat tggcgacgaa caatccattg aagacgactt
     9121 gtccacctac agttcacatt tgttgaacat gaaatttttt attcgctaca gcaacgacaa
     9181 aacattactg ctgattgatg aattcggttc cggaaccgaa cctcaattgg gtggcgcaat
     9241 agctgaagcc gtactggacc gtatcaatcg caacaaagct ttcggcgtaa tcaccactca
     9301 ttacaccaat ttaaaacaat ttgctgaaga aacggaagga atagtcaacg gagcgatgct
     9361 cttcgaccga caaaaacttc aaccgctttt ccagcttcaa attggcatgc cgggtagctc
     9421 gtttgccatt gaaatagcga gaaaaatagg attgcccgaa gatattatcg atcaggcatc
     9481 aaaaaaaatc ggaaaagatt atctcaatta tgaccgttat gttcaggaca ttattcgcga
     9541 caagcgttat tggcaaaaca aaagagaaca aatcaaactg aaagaaaaac aaatggagga
     9601 aactatccgc cgttacgaaa ccgaattgac tgaactgaaa cggcagcgga cggaaatcat
     9661 acaaaaagct aaagaagaag cccaaaatat tctcgaagag acaaatgcca aaattgaaaa
     9721 taccattcga caaataaaag aagcacaagc agaaaaagaa aaaaccaaag aaatcaggaa
     9781 agaactggaa aatttcaaac aaagtgtcaa tcagtctatt gataatcgat tcagcgtaaa
     9841 tcccgctgct gtttcaacta aaaagaaact tgtaaaaaaa gtaacaccca aagaagaaat
     9901 ccataaaaaa gatgtacagg aagaaaaaat ttctgttggc agcaacgtca ggctgaaaaa
     9961 tcaaggtact acaggaactg ttctcgaaat taatggaaat gaggctattg tcgctttcgg
    10021 gcaaatgaaa tctacggtgc cgttctcaaa actcgaacca ctgagccgca ccaaacttaa
    10081 aaaggaaaat caaaacaaaa aaaatatcca tgtcaatacc atcgaagccg ttcggcaacg
    10141 caaactcgag ttcaaaaccg aaatcgatgt acgcggcatg cgtagtaacg aagctttgca
    10201 agccattacc tactatatcg acgacgccat tatggttgga gtcccttcgg tacgcattct
    10261 tcatggtacc ggaacaggtg ctttgcgcca aattattcgg gagtatttac gcactatccc
    10321 ggctgtacga aactttcacg acgaacatat ccaattcggt ggctcgggta ttacagtagt
    10381 ggagttggaa taaaaataaa atttctttta atttttttag gtaaaaacaa gataaattaa
    10441 aacaaacagt atggaattaa aaaattgaat gctgaaatta ttctgaaaaa aaacgaaaaa
    10501 aaaaattatt ttgataacaa tatatttaaa atattcataa ctttgcagaa attttcgtca
    10561 tagaagttca catgttaaat ttttaaatta tttttgaaaa atgaaaaaag tattgttgtt
    10621 ttgtgctgtt atttttgcat ttgcagcttg tgctcctaaa gaaagcaaag aagcctcaac
    10681 cacagaagat tctgtagttg ttgttgatac tgttgttgtt gacagtatgg tggttgacac
    10741 taccgctgct ccaatgaagt aaaaatgccg tttttttttt gtaatcgagt aggtgagatt
    10801 ttcattttct tcctactcga ttatttttta agttatttta aagttcccaa gctaatgcac
    10861 tggcacccaa tacagctgca tccgattctt tcagttcgga aaacaagatt ttgattttac
    10921 ctttccaaat aggaagaaga tttttttcca tattttctat aataggattt aaaataaact
    10981 ctcccgcttt tgataaacca ccaaaaagaa taatggcttc cggagaactg aaagcaacaa
    11041 aatcggcaaa tgcttcacct aaaatttttc cggtaaaatt aaaaatgtct attgccacct
    11101 gatctccttc cttagcagca tcataaacat cttttgaagt aatatcggca agaggaatat
    11161 tacgcaagat actgtcttga ttggtagttt caagaatttc acgagctgtg cgtgccacac
    11221 cggtagcaga agaataggct tccaaacaac cattttttcc acagccacat aaacgcccat
    11281 tgcggatgga ggttgtatga cccaattcac ctgcaaatcc gtcatgaccg taaaccagct
    11341 ttccgtcaat aacaattcca cttccaacgc cggttcccaa tgtaatcatg ataaaatctt
    11401 tcatgccgcg agcaattcca taagccattt cgccgatggc tgcagcattg gcatcattag
    11461 tcaaagccac tggaacgcca aatttttcgt taagcagttt tgcaaaagga attactcctt
    11521 tccatggcaa attgggagca tattcgatgt tgcctgaata ataatttcca ttgggagctc
    11581 caaccccaat ccctcttatt tgatctatac cgccttcagc atcaatcaat ttcattaatt
    11641 catggtaaat atcatttaca taaagattga catcatcgtg aactgcagtc ttgatggcac
    11701 ttttagaaat cacatttcca cgaacgtcca caataccgaa aacggtattg gttccgccca
    11761 tatctattcc gataacataa ggtttttcca taatacgata attttattga aatttattcc
    11821 acttcaacgg gaatatcttt attaacattt ttagaaccaa ttaaagcata gaacaaaata
    11881 tacagcaatc caaccaaaat tacaatgtag cttgcctgat aagtagaaat gtcggcaatc
    11941 cagttttgca gaagtggtaa aatgccacca cctactacca tcatcataaa tattcctgaa
    12001 gcagcagccg tatattttcc aagtccttct actgcgaggt tgaaaatact accccacata
    12061 atggaagtac acaagccgca aagaacaagg aataaagcaa tgagcggtac ttgcaccatg
    12121 ctgaatgatg aaccggtaaa tacaggcatg gaagtagtaa tatctttcgg caataaaatg
    12181 gcaagcaaca ccaatgcaat tcctaatgcg gaaacaaatg taagcatggt tttactagaa
    12241 acttttccac caaccaaact accaagaaaa cgtccaataa gcatgagaaa ccaataagtg
    12301 cctgccacaa aaccggcaat ggcagccgct ttttgaggat ctaaagaagc tcctttaatt
    12361 gttgtatcgg caaggaagaa atttaacgtt ccgggtatgc ccacttctac tcctacataa
    12421 ataaatatgg caatggatcc cagtacaaaa tgacggaaat tccacggtga atgttcgtat
    12481 ttgatttcgg accttttctt tctgttttca tccgggtctg caataggaat aaacaaaaga
    12541 ataagaaagg caagtgcaaa aacgcccaaa gcaatataaa gcacaggatt tacgttgtta
    12601 atggtaactt cacctgacaa aatgcgttct ttggttatat caccaatcaa agctccaaca
    12661 aacataggcg taatggttgc agccaaagaa ttgaaagttg ctccgatttg aatcaactga
    12721 tttcctttgt tgccaccgcc tccaagtaaa ttcaacattg ggttgacaac tgtgttaagc
    12781 atacaaacag agaaaccggc tataaatgca cccaacaaat aaacaaagaa attggcaggt
    12841 agtgagaaaa gttcagctcc aacaccaata ataccggaca gatactgtgt tagaacacct
    12901 aaaaaaccta ctgcaattgc aacaagagcc gtttttttat aaccaatttt tgaaagcagt
    12961 ttaccggctg gaatacccat aaacagataa gccaaaaagt tcatcatatt tccaagcatc
    13021 ccaaggaaat tcgatccttc aaaagcaggc tgatttttcc aaattacccc aatcggcgca
    13081 gcaagattag taacaaacga aatcattgca aacaggaaaa acatagtgat aatggccacc
    13141 agatttccac tttttttctc tgcattcata ttgtttagaa ttaataaagt taattgtaat
    13201 gaaaatgttt tttgaattaa tttatattga ccgacaaaaa taccattttt ttgtcagttc
    13261 cttttaacag aataaacaaa aaagctttat ccaaacaatg ttttacaaca tatttttaac
    13321 caataaaaac agtttgattt tttatatgta atggaattac aaattcttaa acaaaaaatt
    13381 actcatcgaa cgatacaaat acctacgcgt ttgttttccc aaaatgctgt gatttttgtc
    13441 cgtaaacagc tgcatgttat attgtttgtc agcttctacc aatcgtttta catacatcag
    13501 ggtattttgt gcgtgaacat tatcatcggc cgtaccatga atgatcagca attcaccctt
    13561 taatctatct gctttcatta tgggcgaagc aagatcatat cccttaaaat tttcctgagg
    13621 acgacgcatg tatctttcgg tgtatgcagc attgtaaaat ctccagtcgg ttaccggagc
    13681 tacagcaatg cctgccttaa atacattttc tccactactc atggccatta acgtaagaaa
    13741 tccgccgtaa ctccaacccc aaataccgat tctttgcgga tcaatataat cttgcttgct
    13801 gaaaaaacgg gctgcctctg tctggtcttt tgcttcgaga acgcctaact gttcatacgt
    13861 acattttctg aattctactc cgcgcgctcc agtgccacgt ccatcaacgc aaaccaccaa
    13921 catgttattt tcagccaaat aatattccca gtccacatcc catttgtcta aaaccatttg
    13981 cgaattaggc ccactatatt gaatcaacaa taatggatat tttttattcg gatcaaaatt
    14041 caacggtttg agcatccagc catttaacga aatattttca gaagtcgtaa agctgaaaaa
    14101 ttcctttttg ggcaaatgaa gggtattcca attttccaat atgccgtcag agttttgcat
    14161 ttcacgaatg aaattgccat tgttactata caaaataagt ttatcgggag tttgtagatt
    14221 ggaaaaatta tcgacaaaaa agctgaaggt cgaattaaaa atggcgttgt gaaaaccatt
    14281 cccattggta agtttctttt taacgccttt taagctgaca gaataaatat ctctttgcaa
    14341 aggagaactt tctgccgatt gataataaag cgtttgattt ttttcgtcaa aaccataaac
    14401 ttgcgttaca tcaaaatttc cttttgtcag ttgtttgacc ggcgtgccat tgattctatg
    14461 ctgataaaca tgacgaaagc catctttctc actcagaaaa ataaaatatt gattgtcgga
    14521 agtaaattgg caagcatcaa tattttgata atcaatataa tatttatcgt tttcctgcaa
    14581 tatcagagtg gacagagtag attttggatt ggcaagaaac attttcaact gattttgatt
    14641 gcgattcaac acaaaaacgg ccaactgatc cagtgaattg gtccatttta tcctcggaat
    14701 ataaaaatct tctccaacat tactcaactg aacagttctg atcgcttttg tccgttcctc
    14761 ataaacgcaa acacttactt tggaaatatt ttgtccggct ttgggatatt taaaagaaac
    14821 aagtgatgga taaagcatta aatcgtccat agagtttttg ttttcataca attgaaatga
    14881 aaattgaggc acttcagttt cgtcaaattt tacgaaagcc aataatttgc tgtcggggct
    14941 ccaaacaaaa taacgcgttg cttcaaattc ttcttcatac agccagtcag gcaaaccatt
    15001 gataattttt cccggttcac catcttttgt tatagccgat tcggtattaa aatcgagttt
    15061 tttcaaataa agattgttgt tccttccgaa agcaatatat ctactgtcgg gagaaaaaag
    15121 aggtacttcc tgcgagccgt tatccgaaag cggctgaatt tcgttccttt taatgtcgta
    15181 aacataataa tcggctgtga aagtacgtct gtatcgtttt tttacattgt tataaaccaa
    15241 tagtttggct tcattcggac tgaattcata accattaatt tccttgaatg gggcatcttt
    15301 caagcgatga atactaaaaa tggtatctac caattgaccg gttttgtaat tgtattttag
    15361 cactgcatta tctttcatca gcgtataatt ctctccatct ttcatcgagc ggggaaccat
    15421 gatattattc acattaaact tgccatcaac aatatcgtcc agcaagtcgg caaacgaaaa
    15481 ttgcactgca aatgccaaaa caaaagctaa aaaaatacgt ttcataaaaa acttttaacc
    15541 gttaaattac ctattagttg atttttttct tgtaaattca cacaaaagta gcgtaattca
    15601 tcagttttaa aaattttagg tcaatttttt tgaatggttt aacattatac aagaaagaac
    15661 atccacaaca atacaaaaat acatataaca atgaacgatg acaacatcac aaaccatgaa
    15721 cagaatatta acaaaacagg catttgtttg agtggaggcg gcgctttagg ctttgcccat
    15781 attggcgttt tacaggcact tgaagaaaat ggcattttcc ccgttcagat tgcaggctca
    15841 agtatgggag ccattgtagg aacgctttat gctgctgggt ttacaccggc ccaaatgctg
    15901 caaatgatca gagaagacaa attgtacaaa ataaccaaat taatgtctat ccatcccatg
    15961 tcgttgaaat cgggactatc cacccacaaa atgttgcggg atttgataaa ggaattgatc
    16021 cctcacaaca gtttcgaatt attaaagaaa aaaatgtctg tctgcgtcag caatttgaat
    16081 aaagccgaat gggaaataat tgaaagcggc aaggaactgg ataaatgggt agccgcttcg
    16141 gcaagtattc cgggcgtatt tgaagccatt caggtaaaaa aagaatatta tgtggatggc
    16201 ggattattga acaacatgcc ggcacaagct ctcaaagaga attgtagtac gattatcggc
    16261 gtagatgtga ttcctcacct gaatgaggtt caattaaaaa aaccgattga caccttgact
    16321 tattccatcc gcatcataca acataaaaac gcacaaaaag ggagagaact ttgccaatat
    16381 ttgattgaac cgaaagcgat ccgaaaattt cacgaattca gttttgatgc ttaccagcaa
    16441 atttatgaag aaggctacca atgcactcac acttttatag aaaataaccc ggcgataaat
    16501 aatggaattt agcataccga aataaaaaac tgcctttaga tggtaacatc caaaaggcag
    16561 ttttctgtta gaattttttt acaaatgtcc aattacgaaa ttttttctat ttcttcttga
    16621 agattcaatt ccatcccttt attatcgata aatttatact gcaggaaacc caattcctgt
    16681 gaagctctca ctttacatcc tcgtccgaat ttaattttcc atttgttttt caaaggaatc
    16741 attcctttgg aaatataggc ttcaacgtat ggatgtacat agagagtaaa ttctttaact
    16801 tttaaagtat tgaccaaata atctattttg ctttccaata ggtcagtaaa taaaatagaa
    16861 ggttttattt tccctgttcc gaaacaagta gggcaagctt cctcaatatc gacatgcgta
    16921 tcgggtcgaa cacgttgacg tgttatttgc atcagtccga acttgctgag aggcaaaata
    16981 ttgtgtttgg ctctgtcgtt tgccatcatc tctttcatcc ggtcatacac tttctgacga
    17041 ttgtcagatt catgcaaatc aatgaaatcc actacaataa ttccgcccat atcgcgcaaa
    17101 cggagctgtc gggctatttc gtcggcagca gccaagttaa cttccaaagc gttggcttct
    17161 tgtccgtttc ctgtctttgc ccgattgcca ctgttcacat caataacgtg taaagcttcc
    17221 gtatgttcaa taatcagata agctccattt ttaaatgaaa cggttttacc aaaagacgat
    17281 tttatttgtt tggtaatgcc aaaatgatca aatatcggag tttcatcttt ataaagcttt
    17341 acgatatttt tttgttccgg agcaatccat tcaatgtagt cttgcacttc tgcaaataca
    17401 ttgggatcgt tcacataaat gtttttaaaa ctcgggttaa ataaatccct gataattcct
    17461 acagtacgac ccaattcttc cgaaataaga gtaactccct ttgcttgctg aagtttggaa
    17521 acggcttcat cccagcgttt tacgagcaca ttgagttcat tgatcagttc ggcaactttt
    17581 ttgtcttctg ccactgtacg aacgataacg ccaaaccctt tcggtttgat gctctgaata
    17641 agctgtttaa gacggacacg ctcattttca gatttaattt tctgagaaat aagcaccttg
    17701 tcgttaaagg gaattaacac caaaaatcgt ccggctaaag aaatttcggc tgtcagccgc
    17761 ggtcctttgg tggagatggg ttctttggcc acctgaacca gaatttgttg acccggcttg
    17821 agcaggtcgt tgataaaacc tgatttttca atttccggaa gcattttaac tttttgcatc
    17881 gagggcgctt ttttcgggtc gctgagcgcc tgcaaagtaa actgagaaag ggtattgaaa
    17941 ttgggaccta aatcaagata atgaataaaa gcatcttttt cgtaaccgac atcaacaaag
    18001 gctgcattta agcctggcat aatttttttt acttttccta agtaaatatt cccgacagcg
    18061 aattgttctt ctcgtgtttc ttgggcaagt tcaaccagac gtttatcttc gagcagcgca
    18121 atagaaattt ctgatggctt tacatcaact actaattcgc tattcacttt tttattgatt
    18181 ttggttattc gattttcaac aaaaaaacaa actcaaagcc tcaatgaatg ctttaaagtt
    18241 tgtttccttg aactttaatc atttaaagac ccctcgacct attttttctt gtgtctgttc
    18301 tttcttagtc tctttttacg cttgtgcgta gacattttgt gtctttttct ttttttcccg
    18361 cttggcattt tttaaaattt taatttttga aatgaaataa tttacttcac tacagacata
    18421 aatttctttg caggtttgaa agaaggaatg ttgtgaggtg gaatgataat ggttgtgttt
    18481 tttgaaatgt ttcgggcagt tttctgtgct ctggttttta ccacaaaact accaaaacct
    18541 cttaaatata cattctcatt tttagaaaga gaatctttta ttgcatccat aaaagcttca
    18601 acagatgcca aaacagtagc tttatcgatc cctgtttttt tggcaatttc atttacaatt
    18661 tctgctttag tcatttttgc taatatttta aatatgttaa taaattaaga aaactattta
    18721 ttgaattgaa ataggattgc aaatatattg cttttattct gattaaaaaa acatttgcgc
    18781 tattttttta gctaaaattg atttttttaa tgcaagactg aaatataaaa tgaaatttta
    18841 catggaagca aacattaaaa tttctttatt caacgtaaaa tcaaattgtt tattatcagt
    18901 aaaatataaa catcggcttt gaattaattt ctttttttga attgtccaat ggaaaaaatt
    18961 tttaatccat tgaaaataaa ctgctaaaga aaaagttata aattaaaaaa aacaaacaga
    19021 tcattgaact ttcgtagccg tataaatatt tttttgatgt aaatttgcac cggaaaactt
    19081 tatttttcac agcgatataa aaaacctttt ctaatggacg tcaaaataga atcgagttgg
    19141 aaagaagtac ttcaatcgga atttgaaaaa gagtacttca aacagcttac cgactttgta
    19201 cgaaaggaat ataaaacaaa aaaaattttc ccgccgggtt cacttatttt taatgctttc
    19261 aatctgtgtc cgtatgataa agtgaaagta gttattatag gtcaggatcc gtatcatggt
    19321 cccggtcagg ctaacggttt atgtttttcg gtaaacgagg gcgtggattt tccgccttcg
    19381 ctggtcaata tttataagga aatagaaaat gaatttgcca aaccaatgcc caaaaatggc
    19441 gatttaagct cttgggcaaa acagggcgtg ttgctgctca atgccattct caccgtgcag
    19501 gctcatttgg ccggttctca tcaaggaaaa ggttgggaaa catttaccga cgcagtaatt
    19561 cggatattgg ccgaaaaaaa ggaacatttg gtgtttttac tttggggaag ttatgcccaa
    19621 caaaaaggcc tatatatcga caccaataaa catttggtgc tgaaatcgcc tcatccttct
    19681 cctctttcgg cttatcgggg atttatcggg aatcgacatt ttcaactcgc caatcaatac
    19741 ctgaaagagc atggtatgag cgaaatagaa tggtaaatcc tctcaaaaaa agtaaatcaa
    19801 aacaataaca attaaacttt tctataatta atcaactata caaaaatcat ttaaataata
    19861 ttttatggca ttgcaggaag aaatggagaa acagggtaat ttccttttca agtatcgtgg
    19921 atatttacct ttgattattg tagcagtagg gcttgcggtt tttattgaac cacatgacgg
    19981 tcatacattt ttttgtattg aaatattcga ccgttgaatt tatcggtttg tttatttcac
    20041 ttttcggact tttcataaga gcttatgcag tgggacatac gccggcaaat acatcgggac
    20101 gaaacaccaa agaacagttg gcggatgaat tgaataccac cggaatttat tctatggttc
    20161 gtcatccact ctatttgggt aattttttca tgtggcttgg tgctgctgtc ctgacgaaaa
    20221 atggctggtt tatcgtggct ttcattcttc tttactggat ttattacgaa agaatcatgt
    20281 ttgccgaaga acagtttctg cgtcgaaaat ttggttctgt ttataccgat tgggccaaca
    20341 aaacgccggc ttttattcca agctttaaaa aatacattcc ttcaaaatat cctttcagct
    20401 ggaagaaagt actcaaaaaa gaaaaaaacg gcttgctggc tgtatttgtc ctcctgtttg
    20461 tatttcacaa tatcaattat tcttttctaa cgaatcatat ccgtattgat ttgaactgga
    20521 tagcttgggg aacatttgca gcaggcatcc tttatctggt attgaaattt ttgaaaaaga
    20581 aaacttcgat attgaacgaa acgggaagat aaatctgaaa aatatttttt acaaaaaaac
    20641 cggtgaaatt ttctaccggt tttttttgta tgtatggtat taagcttgca ggatgcgagt
    20701 tccggtatgt gccgagttga taagaaacat tagcgaatgg caagggtgtt gcgggtgac
//
LOCUS       DAOSIB010000032        20299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_2887_length_20299_cov_11.279688, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000032 DAOSIB010000000
VERSION     DAOSIB010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 20299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20299
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2887_length_20299_cov_11.279688"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="PLD44_04450"
     CDS             <1..1449
                     /locus_tag="PLD44_04450"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III-family protein"
                     /protein_id="HOF98618.1"
                     /translation="PYYQRYAIWPFMVFAQVIENNQPELKIFSYRDSVLLKATNTLIQ
                     CAYNGDIFYLNDALKKNYKTQEIVYAVDIAYKNNPKNTFLLDIAQQQKSFIVSDAGIA
                     TAKSLNIVKYEPFQFRSVLLRDGANGDEGALGIIRAGKKDKTQMCLTFKATSHGLSHG
                     HYDKLSISMYDGGNFVLTDYGAVRFLNIEPKSGGNYAKENHSWAAQTIAHNTVTVDET
                     SNFNGNIKVSSLHHSDIQYYDFSSNQIQIISGCENNAYKNVKMQRTVAVIENKTFSYP
                     IVIDIFRIISDSTHTLDFPFYYRGQMISTNIQYQKFTAQLNPLGTKNGYQHLWVEAIG
                     KNEKPVTQFTWVEGNRFYTITTLGNSNTEYLMTRTGAGDPNFNLRNETAFMMRQPMVK
                     KHTFVSVVEPHGFYDENKEITENFQTSIRKIELLADNEEYSAVKISTVDNNTFLLIAL
                     NKDFDKDREHSIKIDNQTVQFKGNYYFNELTK"
     gene            1462..1806
                     /locus_tag="PLD44_04455"
     CDS             1462..1806
                     /locus_tag="PLD44_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOF98619.1"
                     /translation="MKTRSLNFLHASQIEPEITGEGVSRQILGYDGQIMMVKVHFKKG
                     AVGYIHEHFHSQTTYVASGKFEVSINGEKQILEAGDGFYIEPDAPHGAICLEDGILID
                     VFSPMRMDFLKI"
     gene            1811..3274
                     /locus_tag="PLD44_04460"
     CDS             1811..3274
                     /locus_tag="PLD44_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF98620.1"
                     /translation="MKIKGLRWWIIGLIMVITIINYLDRGTLNYMWVTNVEYSIVDQF
                     STNTKNYEAVFLKEKNSYLLKNKKGETAEVISSHLLKKKDKKVVVNREGIAYDLGIID
                     KNTTPDEASKKAKGILGTITIFFMIAYGISQLFSGKMYDKIGTRKGFVISAAIWGMAD
                     ALTSLAKGKFSLTAFRMMLGLGEAGPWPGAVKSNAEWFPQKERAFAQGLFNAAASAGS
                     ILAPIIILMLYLLLGWRLTFLIVGSLGLIWIIPWLIINKKGPKDHPWITEKEREYILT
                     GQPEAQHVNEKGKSWKELLSNKKNYSVILGRFFLDPIWWMFVTYLPLYLVEQFNLNIT
                     ELAFSAWVPYFGAMIGSLTGGWFSGYLIRSGKTVDKARKIAMIVGGLIIIPSVIASVL
                     ASNAILAVVLMAFVLGGFQFTMTNIQTIPSDLHSGPSVGSLAGLGGAAAVLGTILAIL
                     FSAQITSWPLLFGLLAALVPLSLGSIFLTVGKIEHIK"
     gene            3296..4048
                     /locus_tag="PLD44_04465"
     CDS             3296..4048
                     /locus_tag="PLD44_04465"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOF98621.1"
                     /translation="MKLKGKVAIVTGGARDLGRAVSIKLASEGAKVVVNYFDNLEDAK
                     ETLKMIQENGGEGIIVQGDMTKAEEVKKLFDAGIAAFGNRIDILVNVVGGICGRKEIT
                     EQDEAWYNLLMDVNMKSCWLCTREVVPHMTNGGAIVNFSSLAARDGGGQGASMYASAK
                     GAVMTFTRSMAKELGPKGIRVNAVAPGTIATSFHDRFNTPENREKMKAIYPLRREGDA
                     GDIADLVLFLVSDDSDYITGTNIDINGGIFFS"
     gene            complement(4131..4808)
                     /gene="trmD"
                     /locus_tag="PLD44_04470"
     CDS             complement(4131..4808)
                     /gene="trmD"
                     /locus_tag="PLD44_04470"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOF98622.1"
                     /translation="MRIDIISAVPQLLESPLNHSIVKRAQEKHLVEIFVHNLHDYSYD
                     KRRNVDDYAFGFSAGMVLRIEPIDRVISKLKQERNYDEIIYTSPDGEMFNQKIANQLS
                     LCENIIILCGHYKGVDQRVRDHLITREISVGDYVLTGGELPAAIMTDAIVRLLPGAIG
                     DETSALLDSFQDNLLAPPVYTRPADYKGWKVPDVLLSGHEANIRQWLHAQSVERTRRR
                     RPDLLEE"
     gene            complement(4852..5766)
                     /locus_tag="PLD44_04475"
     CDS             complement(4852..5766)
                     /locus_tag="PLD44_04475"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOF98623.1"
                     /translation="MPKATFTILGCGSALPTKKNFPSSFLLDLREKQFLIDCGEGAQI
                     RLRQMGLKTNRLGHIFISHLHGDHCLGLLGLISSFSMLHRTADLHIHAHPDLEKIMDV
                     PLHYFSAETPFQLFFHPFNPQVNELIYEDRTVKVSTIPLKHKVPTCGFLFEEKPLERH
                     LIREMIDFYRIPVSQLHKIKQGEDFVTEDGEVVPNERLTYENVPPKRFAYCSDTAYDE
                     SIVPIIRQVDCLYHEATFLEEDIVRADKTLHSTALQAAEIAKKAEVGKLIIGHYSARY
                     ADQSVLLNEAASVFQQVIAGQDMGVYEI"
     gene            complement(5833..6585)
                     /gene="lptB"
                     /locus_tag="PLD44_04480"
     CDS             complement(5833..6585)
                     /gene="lptB"
                     /locus_tag="PLD44_04480"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOF98624.1"
                     /translation="MSNDEMVLRTEHLVKRYGKRTVVDDVSIEVRQGEIVGLLGPNGA
                     GKTTTFYMVVGLVTPNAGKIFLNDIDITKYPVYKRAQSGIGYLAQEPSVFRKMTVEDN
                     IKSVLEMTKFTKEYQKKKLETLIEEFNLEHVRKNIGDRLSGGERRRTEIARCLAIEPR
                     FILLDEPFAGVDPIAVQDIQDIVWKLKDKNIGILITDHNVDETLAITDRAYMLFDGRI
                     MFQGSALELSENPIVREKYLGRDFELKKKTKV"
     gene            6773..7228
                     /locus_tag="PLD44_04485"
     CDS             6773..7228
                     /locus_tag="PLD44_04485"
                     /inference="COORDINATES: protein motif:HMM:NF025698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HOF98625.1"
                     /translation="MKVEIFCPITNKITDESVARLNAGFTVVLLLSYFVTSNIVPVVF
                     LLLDFTARAIERPEWSILSKTSKWILKKLHVESKLINAGPKIFAARIGSFFSLITILF
                     FWLKLPSISIALAAVFFVFALLESAFNYCVACRIYPFIYHALQAKDKEK"
     gene            complement(7256..8752)
                     /locus_tag="PLD44_04490"
     CDS             complement(7256..8752)
                     /locus_tag="PLD44_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOF98626.1"
                     /translation="MELAVEFYFLVASVLLILSIVAGKTGYRFGVPALLLFLLIGILA
                     GSDGLGIEFNSPKQAQLVGVVALNIILFSGGMDTRFSEIKPVLWQGIILSTFGVLLTA
                     FITGCFIYWLTNHLFTAITFSLLESMLLGSIMSSTDSASVFSILRSKKLSIKHNLRPL
                     LELESGSNDPIAYLLMIIFIQLLQSSDISSGEVVAFFFRQFIIGAICGYILSKMVIFI
                     INRIRLDYEGLYSVLLLSFLFFLYSFTSILGGNGYLAVYIGGLFIGNQRFVHKKSSLK
                     FFDGLTWLFQIIMFLTLGLLVNPHELLPIAGAGILIGLSMIVISRPISVFILLSPFRK
                     INFKSKLFVSWVGLRGAVPIIFATYPLMSGIPQARMMFNIVFFITILSLVLQGSTIPF
                     VARLLKLSMPSKDKPKLKEFDIDLPEDIKSAISEITVTEEMLQKGRKLMDITLPDQTL
                     AVMIKRNDHFFVPRGNTKLEIGDTVLLIADNDEIMKETFKKLGVDKEF"
     gene            complement(9068..10609)
                     /locus_tag="PLD44_04495"
     CDS             complement(9068..10609)
                     /locus_tag="PLD44_04495"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOF98627.1"
                     /translation="MLSQEDVFKKLIAHCKEYGFVFPSSEIYDGLSAVYDYGQNGVEL
                     KNNIKKYWWDSMVLLHENVVGIDAAIFMHPTTWKASGHVDAFNDPLIDNKDSKKRYRA
                     DVLIEELIAKYDEKIEKEVEKAAKRFGENFDENLFRATNERVKEHQAKRDEIHRRYVK
                     ALNDNDLAELRAIIIDNEIVCPVSGTKNWTEVRQFNLMFTTEMGSNADSAMKIYLRPE
                     TAQGIFVNFLNVQKTGRMKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVRP
                     GEEMKWFEYWKAYRMKWHKALGLGDHKYRFHDHDKLAHYANAATDIEFELPFGFKEIE
                     GIHSRTDFDLKNHEKYSGKNLKYYDAELKESYTPYVIETSIGVDRTFLSIMASAYTEE
                     KLEDGETRVVLKLPPVLAPVKIGIFPLIKKDGLPEVAHKIFEDLKFDFKCFYDDKDSI
                     GKRYRRQDAIGTPFCITVDHQTLEDETVTIRYRDSMKQERVKIEALSAILNEKGSMKK
                     LLKQL"
     gene            10868..>11239
                     /locus_tag="PLD44_04500"
     CDS             10868..>11239
                     /locus_tag="PLD44_04500"
                     /inference="COORDINATES: protein motif:HMM:NF023205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3316 domain-containing protein"
                     /protein_id="HOF98628.1"
                     /translation="MIKHSSCRFGLIKPSLIAGILIIVCFSINAQSSNQSEKALTTST
                     NSFGFSTINMLDSYLSPLKYSGSGFRYENDNRKFVSPENISLSKENHFKMNFGLTENP
                     AQTSEMWYLGMNFSWGMHHHFY"
     assembly_gap    11240..11339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11340..11624
                     /locus_tag="PLD44_04505"
     CDS             <11340..11624
                     /locus_tag="PLD44_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98629.1"
                     /translation="SYYEIFELQNFSNTLHFTSLHNKLSLTENLSLDIPFNHTTLRFG
                     FRNENLKFEANQMIFKRNDLSFHVGYIYDFYKFEGTKNRAPSGFNSTYNY"
     gene            11640..12650
                     /locus_tag="PLD44_04510"
     CDS             11640..12650
                     /locus_tag="PLD44_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOF98630.1"
                     /translation="MKKHIILLSLLGMLFHSCMDEPVIQPNSYEGNFNALWEIIDTKY
                     CYLDYKNIDWDSIYTAYHQRIPYIKTDLDFFDFLGKMLEELKDGHVNLYSGFDVSRYW
                     KWYTDYPENFSSDIIYSDRYLGENYRIAGGMRYQKIDNGNIGYIYYGDFTSDFSGTNI
                     GYIFKYFSNCKGIILDVRNNGGGYMSGAEQLASYFFTEKTLTGYLCHKTGPGHTDFST
                     PIKLFTPANKNVQWLRPVVILTNRMTYSATNDFVNRMRKAPYAIILGDKTGGGGGMPL
                     SSEIPNGWMVRVPTSPMFDANMQHIEWGIDPDINIALKPEDAARGYDTLIERAVALIK
                     FL"
     gene            12702..13241
                     /locus_tag="PLD44_04515"
     CDS             12702..13241
                     /locus_tag="PLD44_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOF98631.1"
                     /translation="MQKLKVTEMNRLTVEEFKKKEKTPLIVVLDNVRSLHNVGSIFRT
                     ADAFLVQAVYLCGITSTPPNAELHKTALGAENSVEWKYFEETHKALEELRIMGYTIFA
                     IEQARESLPLNSIELNPANKYAIVLGNEVKGVQQTVVDACDGCIEIPQYGTKHSLNVS
                     IAGGIVVWEFFKKLMEERN"
     gene            complement(13869..15623)
                     /gene="sppA"
                     /locus_tag="PLD44_04520"
     CDS             complement(13869..15623)
                     /gene="sppA"
                     /locus_tag="PLD44_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOF98632.1"
                     /translation="MKQFFKYTLATILGVILAGIILVAILFGIAGGIAGQKSAPTKLK
                     PNSVYELKLNGVLVERSEEDPFSIVLAQAMGNEDNLTIGLNDILSNIQKAKTDENIAG
                     IYLNFGTLSAGYASMKEIRDALKDFKKSGKFVVAYADNYLQHEYYLASVADKIFLNPQ
                     GMLDWKGLASEITFYKNTLDKLGIEMQVVKVGTFKSAVEPYVNTKMSDANREQVTVYL
                     NSIWNTLLSEVSDSRKIPFEKLNVYADEMLTFQPTEKAKQYALVDSLVYQDEIDFIVK
                     GFLKDSTANINYVGHEAMCKVPDASKYDRNKIAVLYAAGAIDNGSDGIKSKKLVKTIN
                     EIAKDNSVKAVVFRVNSPGGSAYGSEQIWHALSQLKQKKPLIVSMGDYAASGGYYISC
                     IADSIVAQPNTITGSIGIFGLIPNIEGLDKKIGLTFDGVKTNKMSDAISINRPFRPEE
                     RDLMQNYVNRGYELFVKRCADGRKMSVEQIKAIAEGRVWTGKDAMEIGLVDKLGTIND
                     AISMAVKKAKLTSYQIKEFPKIETFSEKLLKELSDGTETRIEKLKLGEHYQMFKMIEQ
                     LDKMDALQARLPYELTIR"
     gene            complement(15932..17431)
                     /locus_tag="PLD44_04525"
     CDS             complement(15932..17431)
                     /locus_tag="PLD44_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HOF98633.1"
                     /translation="MQNKFKFVSAEEAATYVQHGDVVGFSGFTPAGCPKAVPVAIARR
                     AETFHAEGKEFKIGMYTGASTGVSVDGALAKADAVLFRTPYQSNKDMRNAINAGKAPY
                     FDLHLSTMAQELRFGFLPKPKFAILEVCDWNENGELVLTAAVGISPTIAEMADHIILE
                     WNHYHPKELKGFHDIYQPQDPPYRREIPIYKPSDRIGSEILKVDPAKILCIVETNLPD
                     EVSDFAPLDEVTQQIGNNVATFLAGEIRAGRLPHDFLPIQSGVGNIANALLFSLGKNE
                     EIPPFEMYTEVIQDAVIELMLEGNIKFASGCSLTVTDAVLQKIYQNIDFFRDKLVLRP
                     QEISNNPEMVRRLGLITINTALEADIFGNVNSTHVLGTKMMNGIGGSGDFTRNSFISI
                     FTTPSTAKNGNISSIVPMVSHVDHTEHSVNVLITEYGIADLRGTTPKERAEIIIKNCV
                     APEYKQMLRDYLAISGSSHTPNNLKACFGMHIQLEKTGSMLGTNWDDFK"
     gene            complement(17952..18875)
                     /locus_tag="PLD44_04530"
     CDS             complement(17952..18875)
                     /locus_tag="PLD44_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOF98634.1"
                     /translation="MKILVATDKPFAKVAVDGIRAEVEAAGYELALLEKYTEKQQLLD
                     AVADADALIVRSDIVDAEVIKAAKKLKIVVRAGAGYDNIDLDAATQANICVMNTPGQN
                     SNAVAELVFGLLIYAVRNFYNGSSGTELKGKKLGIHAYGNVGRNVARIAKGFDMQIFA
                     YDAYCPAEVIKNDGVTPLTSVEELYASCDMVSIHIPATPETKKSINASLLQKLPKGAV
                     IVNTARKEVINEEDLINCMELRNDIKYVTDIMPDQHAVFAEKFPVRYFSTPKKMGAQT
                     AEANINAGIAAAKQIVSFFKTGDERFRVNKK"
     gene            complement(18964..20031)
                     /gene="serC"
                     /locus_tag="PLD44_04535"
     CDS             complement(18964..20031)
                     /gene="serC"
                     /locus_tag="PLD44_04535"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOF98635.1"
                     /translation="MKKHNFNAGPSILPREVIEKTAQAVLDFNGSGLSILEISHRSKD
                     FQAVMDEAIALFKELLEIPSGYSVVFLGGGASLQFCMVPFNLLEKKAAYLLTGTWASK
                     AYKEAKGFGEVIEVASSKDANFSYIPKNYVIPSDVDYFHITTNNTIFGTEILTDIDSP
                     VPLVADMSSDIFSRPVDVSKYGVIYGGAQKNLGPAGLAFAIVKEELLGKVSRHIPTML
                     DYRIHIENGSMFNTPPVLPIYSALETLRWLKANGGLAAMEKKNIAKAELLYNEIDRNK
                     LFVGTAAKEDRSRMNVCFVMNPEYQELEAEFLKFASDRGMVGIKGHRSVGGFRASIYN
                     ALPIESVQALIDCMQEFEKLH"
BASE COUNT         6444 a         3971 c         3671 g         6113 t
ORIGIN      
        1 ccttattatc aacgttatgc tatttggcca tttatggttt ttgctcaagt gattgaaaac
       61 aatcagcccg aattgaaaat ttttagttat agagacagtg ttttgttaaa agcaaccaat
      121 actttgattc agtgtgctta taatggagat attttctatt tgaacgatgc tttaaaaaag
      181 aattataaaa cacaagaaat agtgtacgca gttgatattg cctataaaaa taatccaaaa
      241 aatacttttc tacttgatat tgcacaacag caaaaatcat tcatcgtatc ggatgcgggc
      301 attgctacag caaagtcgct gaatattgtg aaatatgaac ctttccagtt tcgctcagtt
      361 cttttgcgag atggagctaa tggcgatgaa ggagcattag gcattatcag agcagggaaa
      421 aaagataaaa cacaaatgtg tcttaccttc aaagcaactt ctcatggatt atcacatggt
      481 cattacgata aactgtccat ttctatgtat gatggaggaa atttcgtttt aaccgattat
      541 ggcgctgtcc gctttctgaa tatagaaccg aaaagcggtg gaaattatgc aaaagaaaat
      601 cattcctggg ctgcacaaac cattgcccat aataccgtaa cagttgatga aacaagtaat
      661 tttaatggca atattaaagt ttcctccctt catcattccg atattcaata ttatgacttt
      721 tcaagtaatc aaatacaaat tatttccggc tgcgaaaaca atgcctataa aaatgtgaaa
      781 atgcagcgaa ctgtagctgt aatcgagaac aaaacatttt cgtatccaat tgtcatagat
      841 atttttagaa tcatctccga ctctacacat acacttgatt tcccttttta ctatagaggt
      901 caaatgattt ctaccaatat tcaatatcaa aaatttactg ctcaattaaa tccattggga
      961 acgaaaaatg gttatcaaca tctttgggtt gaggcaatcg ggaaaaacga aaaaccggtt
     1021 actcagttta cgtgggttga aggaaataga ttttatacga ttacgacatt aggtaattca
     1081 aacacagaat atctgatgac acgtacagga gctggtgacc caaattttaa tctcagaaat
     1141 gaaactgcat ttatgatgcg ccaacctatg gtaaaaaaac acacattcgt ctcagtagtt
     1201 gaaccacatg gattttatga tgaaaataag gaaattactg aaaatttcca gacttccatt
     1261 cgtaaaattg agcttttagc tgataatgaa gaatattcag ctgtaaaaat atctactgtc
     1321 gacaacaata catttcttct gattgcgctg aataaagatt ttgataaaga cagagagcat
     1381 tcaataaaaa tagataacca gacagttcag ttcaaaggca attattattt caatgaatta
     1441 acaaagtaaa tttttacaga tatgaaaaca agaagtttaa attttttaca cgcatcgcaa
     1501 attgaaccgg aaattacagg agaaggtgta tcaaggcaaa tattgggata tgacggtcaa
     1561 atcatgatgg taaaagtcca ttttaaaaag ggggcggtcg gttatattca tgagcacttt
     1621 cattcacaaa ctacttatgt agccagtggc aaatttgaag tttcgataaa tggcgaaaaa
     1681 cagattttgg aggctggaga cggcttttat atcgaacccg acgctccaca cggagcaata
     1741 tgtctggaag atggaatttt gatcgatgtt ttcagcccaa tgaggatgga tttcttaaaa
     1801 atataaatat atgaaaatca aaggattgcg ttggtggatt attggattaa ttatggtcat
     1861 aaccatcatt aactatctgg atagaggtac attgaattac atgtgggtaa cgaatgtcga
     1921 atattccata gttgatcagt tttcaaccaa cactaaaaat tacgaagccg tttttttgaa
     1981 agaaaaaaac agttatttgc tgaaaaacaa aaagggagaa acagcagaag tgatttcatc
     2041 tcacttattg aagaaaaaag ataaaaaagt agtggtgaac agagaaggga tagcttatga
     2101 tttaggcatc atcgataaaa ataccacacc tgatgaagca tcaaagaaag ctaaaggaat
     2161 tttaggaact attaccatat tttttatgat tgcctatggt atcagccagt tgttttcggg
     2221 taaaatgtac gacaaaattg gtacgcgaaa gggctttgtc atatcagctg ctatctgggg
     2281 aatggcagat gcgctaactt ctttggcaaa aggaaaattt tcacttaccg ctttcagaat
     2341 gatgcttgga ttgggggaag ctggcccgtg gcccggggca gtaaaaagca atgccgagtg
     2401 gttccctcaa aaagaaagag cttttgcaca gggacttttt aatgcagcag cttcagccgg
     2461 ttcaatcctt gctcctatca taattttaat gctttacttg ttgctgggat ggagacttac
     2521 tttcctgatt gttggttctt taggacttat ctggattatt ccctggctga taatcaacaa
     2581 aaaaggacca aaagatcatc cctggattac ggaaaaagaa cgtgaataca ttcttaccgg
     2641 ccagcccgaa gctcaacacg taaacgaaaa agggaaaagt tggaaagaat tactatcaaa
     2701 taaaaagaat tattccgtta ttttaggtcg tttttttctc gatcccatct ggtggatgtt
     2761 tgtaacgtat cttccacttt atttggttga acaattcaat ctgaatatca cggaactggc
     2821 tttttctgca tgggtgccct attttggagc catgatagga agtctaaccg gtggttggtt
     2881 ctcaggttac ctaatccgca gcggaaaaac cgttgacaag gccagaaaaa tagcaatgat
     2941 agttggagga ttaataatta ttccttccgt aatagcttct gtattggctt ccaatgcaat
     3001 attggcagtc gttttaatgg catttgtatt gggtggcttc caatttacaa tgaccaacat
     3061 tcaaactatt ccaagtgatt tacacagtgg gccatcagtt ggttctttag caggattggg
     3121 tggagcagca gccgtactcg gaaccatttt ggccatttta ttttctgcac aaattaccag
     3181 ttggccatta ctttttggtt tgctggcggc gttggtaccg ttgtcgttag gatcaatatt
     3241 tttaacggta ggaaaaatag aacacataaa ataatcaatt aaaaatatta taattatgaa
     3301 attaaaagga aaagttgcca tcgtaactgg tggagctagg gatttaggaa gagcagtatc
     3361 aattaaattg gcttctgaag gtgcaaaagt tgttgtcaat tattttgaca atctcgaaga
     3421 tgctaaagaa actttgaaaa tgatacagga aaatggtggt gaaggtatta tcgttcaagg
     3481 cgacatgaca aaagcagagg aagtaaaaaa attatttgat gccggtatag ctgcttttgg
     3541 aaatagaatt gacatactgg tcaacgtagt tggcggtatt tgtggaagaa aagaaatcac
     3601 cgagcaagat gaagcatggt ataatctttt aatggatgtg aacatgaaaa gttgctggct
     3661 ttgtacccgc gaagttgttc cccatatgac aaatggcggc gcgattgtga atttttcgtc
     3721 actcgcagcc agagacggtg gcggacaagg agcttccatg tatgcctcgg caaaaggtgc
     3781 tgtcatgact ttcactcgtt ccatggctaa ggaattggga ccaaaaggta ttcgtgtgaa
     3841 tgctgttgct ccgggtacaa tagctacttc tttccatgac agatttaata ctccggaaaa
     3901 cagggaaaaa atgaaagcaa tatatccgct tcgacgggaa ggcgatgccg gcgatatagc
     3961 cgaccttgta ttgttccttg tttcggatga ttccgattat atcacaggaa ctaacattga
     4021 tattaatgga ggaatctttt tctcttaaga tagaatatag ttagattgtg ttataaaaag
     4081 ctcctgttga agtagtgcta tcaataggag cttttttcat tacgtaaaaa ttattcctcc
     4141 aacaaatcgg gacgacgtcg ccgcgttcgt tctaccgact gtgcgtgtag ccattgtcgg
     4201 atattggctt catgacccga tagcaaaaca tccggaactt tccaaccttt ataatctgcc
     4261 ggacgagtat aaaccggcgg cgccaacaag ttgtcctgaa acgaatcaag caaagccgaa
     4321 gtttcatctc caatagcacc cggcaacaga cggacaatag catcagtcat gatggctgca
     4381 ggaagttcac cccctgtgag cacataatct cccacactga tttcgcgtgt aatcaaatga
     4441 tcgcgcaccc gctgatcaac acccttataa tgaccgcaaa gaatgatgat attttcacat
     4501 agagaaagct gattggcaat tttttgatta aacatttctc catcaggcga agtgtaaata
     4561 atctcatcat aatttctttc ttgcttgagt tttgaaatta ctcgatcgat aggttcaatt
     4621 ctcaatacca ttccggcact gaaaccaaaa gcatagtcgt caacatttcg tcgtttatcg
     4681 taactgtaat cgtgcaaatt gtgtacgaaa atctccacca aatgcttttc ctgtgctctt
     4741 tttacaatgg aatgattcaa tggactttcc aacaattgtg gaacagcaga aataatatca
     4801 attcgcattt ttcccataaa tcgataatca ttttacattc gagctttccc tttatatttc
     4861 atacacgccc atatcctgcc ctgcaataac ctgttgaaaa acggaagcag cttcattcaa
     4921 aagcacagac tggtcagcat aacgcgctga ataatgtccg ataatcaatt tccccacttc
     4981 ggcttttttt gcaatttcgg ctgcctgaag tgctgtggaa tgaagcgtct tatctgccct
     5041 cactatatct tcttccaaaa aagtagcttc atgatacaga caatctacct gacgaataat
     5101 gggcacaata ctttcgtcgt aagccgtatc agaacaatag gcaaaacgct tgggagggac
     5161 attttcataa gttagtcgct cattcggaac aacttcccca tcttccgtaa caaaatcctc
     5221 tccctgcttg attttatgca actgcgaaac agggatacgg tagaaatcga tcatttctcg
     5281 tatcaaatgt ctttcgagcg gtttttcttc aaacagaaaa ccacaagttg gcaccttgtg
     5341 cttcagcggg atggtcgaaa cttttaccgt acgatcctca taaatcagtt cgttcacttg
     5401 tggattgaat ggatggaaaa acaattgaaa gggggtttca gcagaaaaat agtgcaaagg
     5461 aacgtccatt attttttcca agtcgggatg agcatgaata tgtagatcgg ctgtacgatg
     5521 taacatggaa aaagaagaaa tcagtcccaa caaacccaga caatgatcac catgcagatg
     5581 agaaataaaa atgtgtccca agcgattggt tttcagtccc atttgtcgaa gtctgatttg
     5641 agcaccttct ccgcaatcaa tcaaaaactg cttttcgcgc aagtcgagca aaaaagaact
     5701 tggaaaattt tttttggtag gcaaagccga accacaaccc agaatagtaa acgttgcttt
     5761 tggcatgatt gatttttcaa aaaaagaaaa aaccttttgg catgttttgc tttaacgctt
     5821 gaaccagaaa atttaaactt ttgttttctt tttcaattca aaatcgcgtc ccagatattt
     5881 ttcacggaca atcggatttt cggacaattc aagagcagaa ccctgaaaca tgattcgccc
     5941 atcgaaaagc atataggcac gatcggtgat ggccaatgtt tcatctacat tgtgatcggt
     6001 tatcagaata cctatgtttt tatccttcaa tttccaaaca atatcctgaa tatcctgaac
     6061 agcaatagga tcaactccgg caaacggctc gtcaagcaaa ataaaacgcg gttcgatagc
     6121 caaacagcgt gctatttcgg ttcttctccg ctccccaccc gacaaacggt caccaatgtt
     6181 ttttctcaca tgttccagat taaattcttc tatcaatgtt tccaattttt ttttctgata
     6241 ttctttagtg aattttgtca tttccagcac cgatttaata ttgtcttcta ccgtcatttt
     6301 tctgaaaacg gaaggttctt gggccaaata tccgataccg ctttgtgcac gtttataaac
     6361 aggatatttt gttatatcga tgtcgtttaa gaaaatttta ccggcgttag gcgttaccaa
     6421 tcccacaacc atgtaaaaag tagtcgtttt gccggcacca ttcggtccca acaaacctac
     6481 aatttcacct tgccgaactt caatggaaac atcatctact acggttcttt tgccgtaacg
     6541 ttttaccaaa tgttcggtgc gcagcaccat ttcatcattg ctcataacta ttttttgaag
     6601 ttgcaaattt acactttttt atcttgacaa tcaaacgaat ttttttcgat tgcagcaatc
     6661 ctttgttgga tgctgtacct aaattgtggt agataaaata cattttcaat ggtatttacg
     6721 tgcagttgaa agagaagtaa ttttgctgta taaattctaa taaaaaaaca tcatgaaagt
     6781 tgaaatattt tgcccgatta caaataaaat cactgatgag agcgtcgccc gattgaatgc
     6841 cgggtttaca gttgtgttat tactgtctta ttttgtaact tcaaatattg tcccggtagt
     6901 tttcctgttg cttgatttca ctgctcgagc tatagaacga cccgaatgga gtattttgtc
     6961 aaagacgtca aaatggatat taaaaaagtt gcatgtcgaa tcaaaattaa tcaatgccgg
     7021 accaaaaatt tttgcagcgc gcattggcag ctttttcagc ctcatcacga ttctgttttt
     7081 ctggctgaag ttaccatcca ttagcattgc ccttgcagcc gtattttttg tctttgcttt
     7141 gttggaatcg gcatttaatt actgtgtagc ctgcagaatt tatcctttca tttatcatgc
     7201 tctacaagca aaagataaag agaaatgaaa aaatatcagg aatgaagaaa cttttctaaa
     7261 actccttgtc tacacccagt ttcttgaatg tttctttcat gatttcgtca ttatcggcaa
     7321 tcaacagtac cgtatctccg atttccagtt tggtatttcc tcgaggaaca aaaaaatgat
     7381 cgttccgctt aatcattact gccagcgttt gatcgggcaa ggtgatatcc atcagtttac
     7441 gacctttttg caacatttct tcggtaacag tgatttcgct tatggccgat ttaatatcct
     7501 caggcaaatc tatgtcaaat tccttcaact tgggtttatc tttggaaggc atggataatt
     7561 tcagcagacg tgcaacaaaa ggaatggtag aaccttgaag caccaacgaa agaattgtga
     7621 tgaaaaaaac tatgttgaac atcattctgg cttgcggtat tccgctcatc aagggataag
     7681 tagcaaaaat tatcggtaca gcgcctctca acccgaccca tgaaacaaaa agtttacttt
     7741 taaaattaat ttttctgaaa ggagaaagga gaataaaaac cgaaatggga cgcgaaatta
     7801 caatcatgga caatccaatc aatatccctg ctccagctat gggaagaagt tcatgaggat
     7861 tgaccaaaag cccaagcgtt aggaacataa ttatttgaaa cagccatgtt agtccatcaa
     7921 aaaatttaag gctgcttttt ttgtggacaa atcgctgatt gccaataaaa agccctccaa
     7981 tatatacagc cagataaccg tttccgccca aaatagaagt aaaactgtaa agaaaaaaca
     8041 gaaacgaaag taagagtaca gaatataatc cttcataatc caatcgaatc cgattgataa
     8101 tgaaaatgac cattttcgat aagatatacc cacaaatcgc gccaatgata aattgacgaa
     8161 agaaaaaggc tactacttca cctgaactaa tatcggaaga ttgtaaaagt tgtatgaaaa
     8221 taatcatcaa cagataagca atagggtcat tgctaccgct ttccaattca agcaaaggac
     8281 gcaagttgtg tttgatggac aattttttgg aacgcagaat agaaaaaacc gaagctgaat
     8341 cggtagaaga catgatgctg cccaagagca tcgactccaa taagctgaaa gtaatggcag
     8401 tgaaaagatg atttgtcaac cagtagatga aacatcctgt aataaatgca gtcagcaata
     8461 cgccaaaagt ggacagaatg atgccttgcc aaagaactgg ttttatttcc gaaaaacggg
     8521 tatccatacc gccggaaaat aaaataatat tcaaagccac caccccaacc agctgagctt
     8581 gtttgggaga attaaactct atccccaatc catcgcttcc ggcaagaata cctataagca
     8641 aaaaaagcaa caacgccggg actccaaaac gataacccgt tttaccggct acaatactta
     8701 aaataagaag aacagaagca accagaaaat aaaattcaac tgcaagttcc atttcataat
     8761 tcgttgatgt tctaacaaac ttaagatttt tttcaaagaa ttaaagtcgt ctatcaaaca
     8821 agttggtttg tgtaccaaaa acctactgaa tacaaatggg gttcatcgat tttaaaaaaa
     8881 tatggaaaaa acttttcttc aagattttac taccttatcg tggcttttgt gtagaaaaac
     8941 aaataatttt ttaagaaacc cacatgtatt acatacacaa acacgaagga atataatttc
     9001 agacatgtga gttcctcaga acataaaaat aaattatatt ctgatggaat taacctttaa
     9061 tccgttttta cagttgtttc aagagttttt tcatactgcc tttttcgttc agaatagcag
     9121 ataatgcttc gatttttaca cgttcctgtt tcatggaatc ccgataacga atggtaacag
     9181 tttcatcttc aagcgtttga tgatcgactg taatacagaa tggtgtccca attgcatcct
     9241 gacgacgata tcgctttcca atggaatctt tgtcgtcata aaaacattta aaatcgaatt
     9301 tcaaatcttc aaaaatttta tgtgccacct caggcagtcc atctttttta attaacggaa
     9361 aaattccgat ttttacggga gccaataccg gcggaagttt taataccaca cgcgtttcac
     9421 catcttcgag tttttcctct gtataagcag aagccataat ggaaagaaat gttctgtcca
     9481 ctccgataga ggtttcaatc acgtagggag tataagattc tttcaattcc gcatcgtagt
     9541 atttcaaatt tttccccgaa tatttttcgt gatttttcaa atcgaaatcg gtacgggaat
     9601 gaatcccttc aatttcttta aaaccaaaag gcaattcaaa ttcaatatcg gtagcagcat
     9661 tggcgtaatg tgccagcttg tcgtggtcgt gaaaacgata tttgtgatcg cccaagccca
     9721 aagctttgtg ccatttcatt cgatatgctt tccaatattc aaaccatttc atttcctcac
     9781 ccggccgaac aaaaaactgc atttccattt gctcgaattc ccgcattcgg aaaataaact
     9841 gacgcgccac aatttcattt cggaaggctt ttcctatttg tgcaatacca aagggaattt
     9901 tcatccttcc ggttttctgg acattcagaa aattaacaaa aattccttgt gccgtttctg
     9961 gacgtaaata aattttcatg gcactgtcgg cattggaacc catttcggta gtgaacatca
    10021 aattaaactg gcgcacttcc gtccagtttt ttgttccact taccggacaa acaatttcat
    10081 tgtcgataat aattgctcgc aattctgcca aatcgttatc gtttaaagct ttaacatatc
    10141 gacgatggat ttcatcacgt tttgcctgat gctccttcac ccgctcattg gtagcccgaa
    10201 agaggttttc atcgaaattt tcgccaaatc gtttggctgc tttttccact tccttttcga
    10261 ttttttcatc atatttggct atcaattcct ctatgagcac atcggcacga taacgctttt
    10321 tactgtcttt gttgtcaatc agcggatcat tgaaagcatc cacatgtccc gaagctttcc
    10381 acgtcgtggg atgcataaaa atagccgcat cgatacccac tacattttca tgaagcaaga
    10441 ccatactgtc ccaccaatat tttttgatat tgtttttaag ttctacacca ttttgtccat
    10501 agtcataaac agcactcaat ccgtcgtaaa tttcactcga cggaaaaaca aaaccatatt
    10561 ccttgcaatg agcaatcagt tttttgaaaa catcttcttg tgaaagcata tcaacattat
    10621 tttagttatc attccatcaa atcgtaattc gatttttttt atccaatacc ttttttcggt
    10681 gaaaaaaatt tcaaggataa aagtttccct gaaagaaatg caaaattaca gcttttttta
    10741 tatttttgtt gattcaagcc gataaaaaag tttattaatt cgctaaattc attctgaaaa
    10801 tcaacgaaac agatatttat caacccacat gatacaaaat acaggtattt ccctcaaaca
    10861 aaaaaatatg attaaacact catcatgtag attcggctta ataaagcctt ctctaattgc
    10921 tggaatttta attattgtct gtttttcgat aaatgcacaa agttcaaatc aatccgaaaa
    10981 agcgttgaca acttccacta acagctttgg attttctacc atcaacatgt tggattcgta
    11041 cctttcgcca ttgaaataca gcggatcggg atttcgttac gaaaacgaca acagaaaatt
    11101 tgtttctccg gaaaatatca gtctttcaaa agaaaaccat ttcaagatga attttggttt
    11161 gacggaaaac cctgctcaaa cgagcgaaat gtggtatttg ggaatgaatt tttcttgggg
    11221 aatgcatcat catttttatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
    11341 cgtattatga aatttttgaa ttgcaaaatt tttctaatac attgcatttc acctcattac
    11401 acaacaaact gtcgctcact gaaaatctat cgctggatat tcctttcaat cacactactt
    11461 tacgttttgg ttttagaaac gaaaatttaa agtttgaagc caatcaaatg atatttaaaa
    11521 gaaacgattt aagttttcat gtcggataca tttacgattt ttacaagttt gaaggaacaa
    11581 aaaatcgggc gccttcaggt ttcaacagca cttataacta ctaatcaaca agttttttca
    11641 tgaaaaaaca tatcattttg ctttcactac tcggaatgct ttttcattcg tgtatggatg
    11701 aacctgttat tcaacccaat agttatgaag gcaattttaa tgctttatgg gaaattatcg
    11761 ataccaaata ctgttatctc gattacaaaa atattgactg ggattccatc tacactgctt
    11821 atcatcaaag gattccgtac ataaaaacag atttggattt ttttgatttt cttggcaaaa
    11881 tgctggaaga attaaaggat ggacacgtaa acctttattc cggttttgac gtcagccgtt
    11941 actggaaatg gtacaccgat tatcctgaaa atttcagttc ggatattatt tattcagatc
    12001 gttatttagg tgaaaactat agaattgccg gcggaatgcg ttatcagaaa atcgataatg
    12061 gaaatatcgg gtacatttat tatggagatt tcaccagcga tttcagcggc accaacattg
    12121 gctacatttt caaatatttt tccaattgca agggaattat tctggacgta cgaaacaatg
    12181 gtggcggata catgagcgga gctgaacaat tggcttccta cttttttact gaaaaaacgc
    12241 tcaccggtta tctttgccat aaaaccggtc cggggcatac cgatttttct acgcccatca
    12301 aattgtttac tcctgccaac aaaaatgttc aatggttgag accggttgtg attcttacca
    12361 accgcatgac ctacagtgct accaacgatt tcgtcaacag aatgagaaaa gcgccttatg
    12421 ccattatttt aggcgacaaa acaggcggcg gcggcggaat gccgctttca agcgaaattc
    12481 ccaacggttg gatggtcaga gtgcctactt cgcccatgtt cgacgctaac atgcaacata
    12541 ttgaatgggg tattgaccct gatataaata tagccttaaa gccggaagat gccgcaagag
    12601 gctacgatac actgattgag agagctgtag cgttgattaa atttttatga aaaatcgaaa
    12661 attgaaataa ataatcgaaa ataaaatttt tgtttgagaa aatgcaaaag ctgaaagtta
    12721 cggaaatgaa tcgcttgacg gtagaggaat tcaaaaaaaa agaaaaaacg ccgctgattg
    12781 ttgttctcga taatgttcgc agtcttcaca atgtaggttc aattttccgc acagcagatg
    12841 cttttctggt acaggcggtc tatttatgcg gaataaccag tacgccccct aatgccgaat
    12901 tacacaaaac ggcattggga gcagaaaatt ccgtagaatg gaaatatttc gaagaaacgc
    12961 ataaggcctt ggaagaactg cgaataatgg gatatacaat ttttgccata gaacaggcac
    13021 gtgaaagttt gccattaaat tccattgaat tgaatccggc aaataaatat gccattgtat
    13081 tggggaatga agtgaaaggc gttcaacaaa cggtagttga tgcctgcgat ggttgcattg
    13141 aaataccaca atacggcacc aaacattccc tcaacgtaag cattgccggg ggcattgtag
    13201 tttgggagtt ttttaaaaaa ttgatggaag aaagaaatta attccctttc ggtttcttaa
    13261 acgggagttt aacttactcc aaagatcttt cgaaattagt tggttttaga acgagggtat
    13321 aaaatgaatg aggaaaatcg ctgatagtaa caagaaatga agaaacctgt gcccaaacac
    13381 cgcaacatgg attggctcat atttagagag tccattgaaa agcactacca aatatcatac
    13441 aaaaaggctc aaatttataa tttgcgactt tataggtcaa gatttgagaa aaatacttcg
    13501 cgcatttaaa aaacacatca aagattattt tgatagttga gaatgagtat caccacgcaa
    13561 ggcttctgaa aaaaattttt gaactcaaac aaaaaattta catttttttg tttcgatact
    13621 tactccatcc aatttcaatt caaaaagccc ggtgattcat aagaaaatcg ataattcaca
    13681 ttatgccttt agaatcgctg aagttgagta aagcaaagct taatcagaat agctactcat
    13741 tttcaaattc caaacgaaga attattgact atttgtgcaa tttattatta aactaaatat
    13801 taaaaagcaa aagaggcaaa accgattttt gatttcacct cttccgaaaa acaaaataaa
    13861 actaatattt atctgattgt caactcataa ggcaaacgag cctgaagtgc atccatttta
    13921 tcgagctgct caatcatttt aaacatttga taatgttctc ctaattttag tttctcgatg
    13981 cgtgtttccg ttccatccga caattctttc agtagttttt cgctgaaagt ctcaatttta
    14041 ggaaattcct taatttggta ggaagtcagc tttgcttttt tcactgccat agaaatggca
    14101 tcgtttattg ttcccaattt gtcgaccagt ccgatttcca ttgcatcttt tcctgtccaa
    14161 acgcgtcctt cagcaatggc tttaatttgt tcaaccgaca tttttcttcc atcagcacaa
    14221 cgttttacaa acaattcgta gccccgattc acataattct gcatcaaatc tctttcttct
    14281 ggcctgaagg gacggttaat acttattgca tcgctcattt tattggtttt tacaccgtca
    14341 aaagttaatc ctattttctt gtctaaccct tcaatattgg ggataagccc aaaaatacca
    14401 atagaacccg taatggtatt tggctgggct acaatgctgt cggctatgca ggaaatataa
    14461 taaccacctg aagcggcata atcgcccatt gaaacgatca atggtttctt ttgcttcaac
    14521 tgacttaagg catgccaaat ttgttcggag ccgtaagcac ttcctcccgg agaatttacc
    14581 ctgaaaacaa ctgcctttac tgaattatct tttgctattt cgttgatggt ttttaccagt
    14641 ttttttgact taattccatc agaaccattg tcaatagcgc cggctgcata aagaactgca
    14701 attttgtttc gatcatattt ggaagcatcg ggaactttac acatggcttc atgcccaaca
    14761 taatttatgt tggcagttga atcctttaaa aaacctttga caatgaagtc gatttcatcc
    14821 tgatatacga gtgaatcgac caaagcatac tgcttggctt tttctgtagg ttgaaaagtc
    14881 agcatttcat ccgcatagac atttaatttt tcaaaaggaa ttttacgact gtcggaaact
    14941 tccgagagca atgtattcca aatggaattg agataaacgg taacctgttc gcggttagca
    15001 tcgctcattt tggtgttaac ataaggttca acggccgatt taaaagttcc aactttcact
    15061 acttgcattt cgatgcccag cttgtcaagc gtatttttat aaaaagttat ctcggaggcc
    15121 aagcctttcc aatcgagcat accctgtgga ttcagaaaaa ttttgtcggc aaccgaagcg
    15181 aggtaatatt cgtgttgcag ataattgtcg gcataagcca caacaaattt tccggatttc
    15241 ttaaaatctt tcagcgcatc ccgtatttcc ttcatggaag catatccggc agataaagta
    15301 ccgaaattga ggtaaatacc ggcaatattt tcatctgttt tggctttttg aatattactg
    15361 agaatatcgt tcaaaccaat ggtgaggttg tcttcatttc ccatcgcttg tgccaagaca
    15421 atggagaaag ggtcttcttc cgaacgttct accaaaactc cattcaattt aagttcataa
    15481 acggaattag gttttaattt tgttggagca cttttttgtc cggctattcc accggcaatt
    15541 ccaaacagaa tggctaccaa aataataccg gccaaaatta cacccaaaat agtggcaagt
    15601 gtgtacttaa aaaattgctt cataaataat gattgttttg atattaatat ggctaagaaa
    15661 caattgataa aaaaggagaa aaatcactga caaaaataga gtttaaagaa aaaatacaag
    15721 ctatttctca atgccgaata cgagtaaatt aacaagttca taaaaaactt tacaaaggaa
    15781 atcaataatt ttctcatttc aaacgattga attcaaattt ttttgaagca gcaaaatttt
    15841 ctaccctttc agcaaaatga aaaagagaag ccttctttta acaaaaaggt tcctcttctc
    15901 aaaaaaatta tgaaaaatct ctcgaaatca attatttgaa gtcatcccag ttggttccca
    15961 acatgcttcc cgttttttcg agctgaatgt gcatgccgaa acaggctttc aaattgttgg
    16021 gcgtgtgact tgatccggaa atggcaagat aatcgcgcag catttgctta tattcaggtg
    16081 caacacagtt tttgataatg atttcagctc gctctttggg cgttgttcca cgtaaatcgg
    16141 caatcccata ttccgtaatc agcacattca cagaatgttc ggtatgatcc acatgagata
    16201 ccatcggaac aatgctgctg atatttccgt ttttggcagt ggaaggagtg gtaaatattg
    16261 aaataaacga gttacgtgta aaatccccag aaccgccaat cccattcatc attttagtgc
    16321 caagaacgtg cgtgctgttg acatttccga aaatatcggc ttcaagcgct gtattgattg
    16381 taatcaaacc caaccgacgc accatttctg gattattgga aatttcctgt ggacgcagca
    16441 ccaatttatc ccggaaaaaa tcaatatttt gataaatttt ttgcaatacc gcatcggtaa
    16501 ctgtcaacga gcaaccactg gcaaatttta tgttgccttc cagcatcaat tcaatgacgg
    16561 catcctgtat tacttccgtg tacatttcga agggcggtat ctcttcattt ttacccaaag
    16621 aaaacagtaa agcattggca atgttaccta ctccggactg aataggtaaa aaatcatggg
    16681 gtagccttcc ggcacgaatt tcaccagcca gaaaagtagc cacattgttg ccaatttgct
    16741 gggtaacctc atccaatggc gcaaaatcgg aaacttcatc aggtaaattg gtttcaacaa
    16801 tacaaagaat tttcgctgga tcaactttca aaatttccga accaattctg tcggatggtt
    16861 tgtaaatggg aatttctctg cgataaggcg gatcctgagg ttgataaata tcatgaaacc
    16921 ctttcaactc tttgggatga taatgattcc attccagaat aatatgatca gccatttcgg
    16981 caatagtggg tgaaatgcct acagcagctg ttaaaaccag ttccccgttt tcattccagt
    17041 cgcagacttc caaaatggca aattttggct ttggcaaaaa tccaaaacgc aattcctgag
    17101 ccattgtgga caagtgcagg tcaaaatagg gagctttccc agcattgatg gcgttgcgca
    17161 tgtccttatt cgactgataa ggagttctga acaacacagc atctgctttt gccaatgccc
    17221 catctaccga tactcctgtt gaagcgcctg tatacatgcc tattttaaat tcttttcctt
    17281 ctgcatgaaa agtttctgcg cgcctggcta tagctacggg aacagctttg gggcagcccg
    17341 caggcgtaaa tccgctaaat cctactacat cgccgtgttg aacataagtt gcagcttctt
    17401 ctgcactgac aaacttaaat ttattttgca taaaaattgg tttatttaat cttgaaaatt
    17461 cgccgtaaaa ttaacaaata tttctcaatc cttcaagaat ttcagtgtca tgggcactaa
    17521 gtttactttt ttataatttc tctaagcgca tgacaaaatt ttaaaatatc gatattgatt
    17581 gtcgaaactt ccatttcgtt ctgtcaatag cactcgagta cggtggatca tgcagtagaa
    17641 gcacaaatat caactgagca attaagtctt aaagcttgta atcagccata aaagccgtat
    17701 tcgccgcaat gaaaaattgc tgtcgcagct gttttgaagc caaaggcgag cttttcaaaa
    17761 caaacggcct gaaaccttac aaacggcgca aataaagagg ctaataaact ttattcgggc
    17821 taagtttata cccttaccga agtgttgaac caaaactgaa gttaaatcat tacttttgga
    17881 agagaccaaa gactttataa taaaaaagtg tcctttgagg acaccatctg gtcatttaaa
    17941 tcaatacaag attatttttt atttacgcgg aaacgttcat ctcctgtttt gaagaacgac
    18001 acaatttgct ttgcggcagc aatgccggca ttaatgttgg cttcggcagt ctgagcaccc
    18061 attttcttgg gtgtcgaaaa ataacgtacg ggaaattttt cggcaaaaac tgcatgctga
    18121 tcaggcataa tgtcggtaac atatttaata tcattacgca actccatgca atttatcaaa
    18181 tcttcttcgt taatcacttc cttgcgggcc gtattgacta tgactgcacc ttttggcaac
    18241 ttctgaagca aagaagcatt gatggatttt tttgtttcgg gagttgcggg gatatgaatg
    18301 gaaaccatat cgcatgaagc atacaactct tcaacagaag tgagtggagt aacgccatca
    18361 tttttaataa cttcagcggg acaataagca tcatatgcaa aaatttgcat atcgaaacct
    18421 tttgctattc tggccacatt tctgccaaca tttccgtaag catgaatacc aagttttttc
    18481 cctttcaact ctgttcctga agacccgtta taaaaattac gcacagcata aatcaacagt
    18541 ccgaaaacca actcggcaac tgcattagaa ttttgccccg gcgtattcat gacacaaata
    18601 tttgcctggg tagccgcatc aaggtcaata ttatcataac cggctccggc ccgaacaaca
    18661 atttttaatt tcttggctgc ctttatcact tcagcatcta caatatcact gcgaacaatc
    18721 aaagcatcgg catcggccac ggcatcaagc aactgttgct tttcggtata tttttcgagc
    18781 agggcaagtt cgtatcctgc ggcttccact tctgcccgaa tcccatccac tgccactttg
    18841 gcaaatggtt tatcggtagc tacaagtatt ttcataagca aacttttttc tctccttttt
    18901 caataaagaa gaattattta tgttaatggt taaaattgta aacagctgtt gctaccaaaa
    18961 gcatcaatgc aatttttcaa attcctgcat acagtcaatc aatgcctgaa cgctttcgat
    19021 aggaagtgca ttgtaaatgg aagcgcggaa accgccaaca gaacgatgtc ctttaattcc
    19081 taccatgccg cgatcggaag caaatttaag aaattcggct tccagctcct gatattcagg
    19141 attcataacg aaacaaacat tcatgcgtga acgatcctct ttggctgctg ttccaacaaa
    19201 caatttattg cggtcgattt cattgtagag caattcggct tttgcaatat ttttcttttc
    19261 cattgctgcc aaaccaccat ttgccttcaa ccagcgcaac gtctcgagtg ccgagtaaat
    19321 gggaagaacc ggcggcgtat tgaacatcga accattttct atatgaattc tgtaatcgag
    19381 catagtgggg atatgacgag aaactttgcc cagaagttct tcttttacaa tggcaaaagc
    19441 caaacctgca ggtcccagat ttttctgtgc acctccataa ataacgccat attttgaaac
    19501 atctacagga cgtgaaaaaa tatcggacga catatcggca accaaaggca ccggagaatc
    19561 gatgtcggtt aaaatttctg ttccgaaaat tgtattattg gttgtaatat gaaaataatc
    19621 gacgtctgag gggatcacat aattttttgg aatatagctg aaattggcat ctttagaaga
    19681 agcaacttct attacttctc cgaaaccttt agcttccttg taagccttgc ttgcccaagt
    19741 accggtaagc aaataagcag ctttcttttc aagcaggttg aagggaacca tgcaaaattg
    19801 caaacttgcg cctccaccca aaaaaactac cgaataaccc gaaggaattt ccaacaattc
    19861 tttaaaaaga gcaatggctt catccattac tgcctgaaaa tccttagaac gatggctaat
    19921 ttccaaaatc gacaatccgg aaccattgaa atccaatacc gcttgtgctg ttttctcaat
    19981 cacttcacga ggtaatatcg atggccccgc attaaaatta tgctttttca tcatttcatt
    20041 aaaaaatttt tttggtttat aagtttctcc ctttgaattt aaagcacaaa agtacacaaa
    20101 ttttaaattc tttcatttct tatggaaaaa ttttttagtt tcaattttaa attttcatat
    20161 aggagtctcc tctgaaaagt tactcaaaga gaattagttt tttaaagaca ttgagataaa
    20221 aaagggtaaa aaatattcaa aaaagttctt tgaaatttgc atatattgtt atatttcagt
    20281 atcttaggag gttgaaata
//
LOCUS       DAOSIB010000033        20286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_2889_length_20286_cov_12.273491, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000033 DAOSIB010000000
VERSION     DAOSIB010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 20286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20286
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2889_length_20286_cov_12.273491"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3591
                     /locus_tag="PLD44_04540"
     CDS             <1..3591
                     /locus_tag="PLD44_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOF98636.1"
                     /translation="DSDGDIWISVEEKGVYLYHYENNTLDHFPVKTPPGGLKGIKLCE
                     NKGKGIWAFPYSMPFLQIDKKSRKIQPFSLNQGNEILYNTGEIGSVIEEQNNLLVGTS
                     LQGLLQINLVERTYNKLLASDETGQPIFVRNILRKGDDLWVATESGIYIYNLYSRDVI
                     NLRHNNVIPYSLSDNAVYSLYEDNEGGVWMGTYFGGVNYFPNRLTYFEIFYPLENENS
                     IKGRRVREFCENTDGRLWIGTEDNGLNLFDPETSKFLPVDSKLNSLYHNIHALFRDGN
                     TLWIGTFSKGLNRYDLKTKTLKTYYKTSEPNSLSENSVFSICKDRQNTLWIGTLSGLN
                     IFNYQNDSFTRIKELEGAFVQDIFEDTDGNIWVATFVKGLFLFNPKTHTWKNFVHTSN
                     AGSLPYNKTTSVFEDSKRRIWITTEGGGFCRYDKKNQSFVTVNSTNGLPNDVVYQIQE
                     DNSGNLWLSTNSGLVKYVPDTGLMENFTVNDGLKTNQFNYKSSYKAPDGTLYFGSIDG
                     FVRFNPAYFKENNLLPPIVLTDFYLNNSPANVSDAGSPLKENISFAEKIRLPYNKNSF
                     TIKYAILNYSGLRPKNVVYKLEGFDRQWIPANGSQSVVYSNLNPGKYRLMISFDTISS
                     GGMNKVSKTLDIVIKPPFWETWWAYLLYFLITIAGIFELINYFRQRTKRIHLEKMRDF
                     EQEKEKELYESKIDFFTNVAHEIRTPLSLIKAPLDQILRNDETVTPDVKDNLKVMSKN
                     TDRLLDLTNQLLDFRKTEAELYKLKEKRTNLSELMKETFDRFTPLAKQKNISFHLNLP
                     ENEVFTKIDAEAFTKIISNLINNAVKYCDSMVNISLEVVAENHPEKLVFKTENDGAVI
                     PERFKNEIFKPFTRLNKEKDKIVTGTGIGLALTKSLTELLKGDITYKVENGLNVFRVT
                     LPFNPLTGNEWIEEKTEMQETPSRKEEDKVEKSLKRPLILLVEDDKGMMEFLTKCLKR
                     EYSVLSASDGKEALEVLKNKNIDLVVSDVMMPEMDGFELTSYIKSHIEYNHIPVILLT
                     AKVNVQSKVQGFEVGADAYIEKPFSVDVLLAQIASLLQNREKFREAFLKNPFKGLNHV
                     ELNKSDEEFLQKLDDIIQENISNPDFNIEDLSEHFFMSRASFYRKIKGILDLTPNEYL
                     RVARLKKAAQLLKEGNQRITEICYLVGFNSPSYFAKCFQQQFGVLPKDFS"
     gene            3699..5510
                     /locus_tag="PLD44_04545"
     CDS             3699..5510
                     /locus_tag="PLD44_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOF98637.1"
                     /translation="MLRIKLLLLSSLFSVITFAQWQPVGEKIKTRWSSQIDVNNVLPE
                     YPRPLMERSEWMNLNGLWNYAILPVGKPAPSAFDGKILVPFAVESSLSGVQKNVGKAN
                     ELWYEREFTIPSKWKNNRVLLHFGAVDWKTDVWVNDVKVGRHTGGYTPFSFEITTALK
                     NGSNKLIVKVWDPTNDGYQPRGKQVNKPEGIWYTPVTGIWQTVWLEPVPDKFITQVKT
                     TPNIDINILNIEVATNNFSASDRIEVKVMDGSKVVATAQSINHLPVEIVMPDQVKLWS
                     PDSPFLYDLEISLWSGTKQIDKVKSYAAMRKYSIKRDENGIVRLQLNNKNLFQFGPLD
                     QGWWPDGLYTAPTDEALKFDIQKTKDFGFNMIRKHVKVEPARWYTYCDRLGMIVWQDM
                     PSGDGGPQWQPHQYFSGLEWLRSPESEANFRKEWKEIIDFLYSNPCIGVWVPFNEAWG
                     QFKTKEIVEWTKEYDPSRLVNPASGGNHYPVGDMLDLHNYPNPALYLYDAQRATVLGE
                     YGGIGWAVKGHLWEPDRNWGYVQFNSAKEVTDEYVKYAEQLMNLIQHGFSAAVYTQTT
                     DVEVEVNGLMTYDREIIKMDESRVKQINQKICNILKN"
     gene            5489..6652
                     /locus_tag="PLD44_04550"
     CDS             5489..6652
                     /locus_tag="PLD44_04550"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOF98638.1"
                     /translation="MQYFKELAICISVLLCFSCQKNNEIAAKSPSLSIRWDQSSLVCI
                     AAEGGYPRVKRLNDGSLLAAYENRKGDVLVKQSRDEGKTWSDSVLVFASFDYIHPSNG
                     LSAKINIANPEFIQLGNDDILFACNFRPNKDEVYPFSIAVKRSRDNGKSWSEQQIVYQ
                     AAPRFIDGCWEPSFLLLPDGRIQLYFANEFPYQQSNEQEISMLESNDNGTSWSADKKT
                     VSFRKGFRDGMPVATTNGNEIFVAIEDNGFDQFKPFIVKNAIADNWKEPVLEESPNRY
                     SALKEPLPSSVYAGAPYLIRTKEGYYVLSYQTTHHRTSNWELSTMEVVVSDKPSDFIS
                     PSQPFAVPLSKEAKWNSLCDLGNGEIAAVSSTNFKSDKIGIWMIKGKIVDNRK"
     gene            6658..7662
                     /locus_tag="PLD44_04555"
     CDS             6658..7662
                     /locus_tag="PLD44_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOF98639.1"
                     /translation="MKRSLGIIFFTLLFFSCKGSEAIIPETESVSTYKNPIIPRSLPD
                     PTVIKASDGNFYLYATEDIRNVPIFRSSDLVNWNFLGTAFTDQTRPTFVSGGGIWAPD
                     INYVNGKYVLYYSMSVWGGEWSCGIGVATADHPEGPFTDHGKLFISDEIGVQNSIDPC
                     YFEENGKKYLFWGSFRGIYGIELSDDGLSVKPGAQKVQMAGTAYEGTYIHKRNGFYYL
                     FASIGSCCEGLNSTYTAVVGRSDNLFGPYLDKSGQSMMENHHEILIQKNSKFVGTGHN
                     SEIVSDDKGQDWIFYHAVWVDHPQGRVLMLDKVQWMNDWPYVTDKCPSLEAEKPYFNE
                     "
     gene            7712..8782
                     /locus_tag="PLD44_04560"
                     /pseudo
     CDS             7712..8782
                     /locus_tag="PLD44_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040227.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
     gene            8801..9874
                     /locus_tag="PLD44_04565"
     CDS             8801..9874
                     /locus_tag="PLD44_04565"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOF98640.1"
                     /translation="MKPENFNEKIDGKQISLFTLKNRQGMTAQITNYGGRVVSLWVPD
                     KKYDFKDVITGYSSLKGFQQSTEVYFGALIGRYGNRIAKGRFELNGKTYQLPINDGEN
                     HLHGGPKGFHTVIWDARPFKNANNEDALELKYFSRDGEEGYPGNLTVKVVYTLTNDNE
                     LKIEYSATTDKPTIINLTNHMFFNLHGFSDGIAKSINSHVLEINASNYTPTDKGLIPT
                     GIIAPVKGTPMDFTVPTPIGERVNENFIDLINGKGYDHNWVLNKKGDKLTEAAVVHEP
                     SNGIQMRVITDQPGVQFYGGNFLNGTERGKYGEVYTYRTSFAMETQHFPDSPNHPNFP
                     STVLNPGQQYQHVCIYKFEVGGN"
     gene            complement(10133..12559)
                     /locus_tag="PLD44_04570"
     CDS             complement(10133..12559)
                     /locus_tag="PLD44_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOF98641.1"
                     /translation="MRQFVFTAILLFWTLMSFGQHWIKGKVLDENGHPLFGADIYLKK
                     EGIGKTTDKNGTFLFENLKRNNYVLQVSYLGYETQWVETTPDKEITIQMNRISIEIGE
                     ITVTSLRANERSAIAYSEIKKEEIESRNLGQDIPYLLALTPSFIVTSDAGNGVGYTGF
                     RIRGTDANRTNVTINGIPLNDAESHGTFFVDFPDLAASLSSVQVQRGVGTSTNGAAAF
                     GASINMQTESVSLEPYADISTSLGSFATNKNSIKVGSGKLNNNFAFDARFSNVTSDGY
                     IDRASVDMQSYYFSGGYFSDKTVLRLLTFGGKEKTYQAWNGVDAEIMKTNRRYNDLGA
                     YTDNNGELKFYDNQTDNYLQTHYHLYWLQKWNSHLHSNVATHLTRGIGYYEEYKTDRK
                     YAEYGLAPAIIGFDTLKTTDLVRQKWLDNYFYGITGALNYEKKNTNFSLGGAANRYDG
                     QHYGKIIWVRNAANLDIGKDWHRSSSIKDDANIYAKLNTETFKNLFASIDLQYRYINY
                     RMDGTDDKYDKINQSMRDITQQHIYHFFNPKFGFTWQPSTRNEFYASYSVSHREPNRN
                     NFTEAGANERPTNETLYDTEAGYRYRSSRFSAGTNFYYMKYKNQLILTGKISEIGEFL
                     TTNIPDSYRAGIELTAGWKINNYLKWDGNCTFSRNKIKNFTEQDIYVYDENWKYIGKR
                     NNELGTTDIAYSPNVIANSIFSFHLKRFEAAFHSNYVSRQYIDNTSDIQRSIDPYFVQ
                     NLNLKYSLAISPIKTLEFLVQINNLFNEKYETNGYTWYSCYLGNERHNELRYFPQAGT
                     NFLASARLKF"
     regulatory      complement(12612..12707)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(13136..13906)
                     /gene="trpA"
                     /locus_tag="PLD44_04575"
     CDS             complement(13136..13906)
                     /gene="trpA"
                     /locus_tag="PLD44_04575"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOF98642.1"
                     /translation="MNRIQQLFTQKKENILSVYFTAGFPKPNDTVPILESLQKYGVDM
                     AEIGIPFSDPLADGPVIQNSSQIALKNGMNLKKLFSQLANIRASVHIPLVMMGYLNPI
                     MQFGFENFCSECKKVGVDGMIIPDLPMDVYLSEYREIVEKNGLDFIFLITPETSEERI
                     HQIDEHTHGFIYMVSSAGVTGTQNSFDHHIDYFNRINSMHLKNPRLIGFGISNKLTRE
                     TASRYSSGVIIGSAFIKALQESSDVESAVKFLIKKLKE"
     gene            complement(13933..14616)
                     /locus_tag="PLD44_04580"
     CDS             complement(13933..14616)
                     /locus_tag="PLD44_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOF98643.1"
                     /translation="MRSVEKNNIVPSRFADTTKKKLIKVCGMKFPENIRAVARLRPDF
                     MGFIFYPKSPRYAEPLNVSVLNELPPEIGKIGVFVNEKLDQILATVYKYHLDGVQLHG
                     SEMVKMCNQLHDTGLLVIKAFPVAEAYNFKVMKRYEKSCDYFLFDAQTPIYGGAGQKF
                     DWQLLENYKEKTPFLLSGGISIEDLEDINKIQHPAFAGVDLNSRFEISPGLKNVELLS
                     NFIDRYRSL"
     gene            complement(14765..15592)
                     /gene="trpC"
                     /locus_tag="PLD44_04585"
     CDS             complement(14765..15592)
                     /gene="trpC"
                     /locus_tag="PLD44_04585"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOF98644.1"
                     /translation="MTDSTLKNNHLSNPGENFLNNILLNKIKEVSKRKLNVSINELEN
                     SNFFDRSTISLKQKLQNSSLGIIAEFKRKSPSKGWIHADANVQEVTIGYNVEGASGIS
                     ILTDTEFFGGNIEDVTTVRNKISCPILRKDFVVDEFQIYEAKAIGADVILLIASALTV
                     FEVKNFSRLAHQLGLEVLLEVHNKEELSYLNENVDMVGVNNRNLKTFQVDTGISANLA
                     AFIPPEFVKISESGISNVETVKELNRLGYRGFLMGENFMKEVQPALALKEFLRQLNE"
     gene            complement(15602..16600)
                     /gene="trpD"
                     /locus_tag="PLD44_04590"
     CDS             complement(15602..16600)
                     /gene="trpD"
                     /locus_tag="PLD44_04590"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOF98645.1"
                     /translation="MKQILNRLFNHQSLTREESAEVMRNIGAGMYNESQIAAFISVYL
                     MRSIELDELIGFRDALMSMAIPMDLSDFDPIDIVGTGGDEKNTFNISTCSCFVLAGAG
                     YKVAKHGNYGATSISGSSNVLEYYGAKFTTDTDVVRRSLEESGFAYLHAPFCNPAMKN
                     VAPVRKNIGVRTFFNVLGPLISPIRPRYQCLGVYNLKMMRLYNYIYQRLGTNYTIVYT
                     LDGYDEVSLTDDCKIINNFGEYVYSPQQLGFQRVKQEELWGGTTVEHAAEIFMNVLDN
                     KASDAQRNVVVINSAFAIQTRCPEKSLEECKAEAIASLACGKAKKAFVKFEEIYHL"
     gene            complement(16679..17260)
                     /locus_tag="PLD44_04595"
     CDS             complement(16679..17260)
                     /locus_tag="PLD44_04595"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOF98646.1"
                     /translation="MKLLVFDNYDSFTYNLVHIVKKLGFTDVEVHRNDRISLEEINRF
                     DKIILSPGPGVPSESGILLDLIKTYASSKSIFGVCLGVQAIAEAFGGSLLNLPQVYHG
                     IASEVSIFDDEVLFRNLPEKIIAGRYHSWVVNDSTLPPCLQVTAKDEQGTIMALKHRT
                     LDVRGVQFHPESVLTPSGEQILKNWLEFPLSGK"
     gene            complement(17346..>18731)
                     /locus_tag="PLD44_04600"
     CDS             complement(17346..>18731)
                     /locus_tag="PLD44_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOF98647.1"
                     /translation="ETPVGIYLKIRDLYTHSVLLESSDYHAAENSYSFIAFSPIGGIS
                     VNHFLVHEYYPDGTKIQTPVTDKMTVADRFDIFLKSFERIEQDNEVPAVVNGLFGYTS
                     YEAVQYFEDIRLSARETVPGSMPGLHYVLFKYIIAVNHFKNELTIIENLLENEKSAMN
                     EIEEILLNNNIAAYEFSGIGSEKCDINDEDYRRMVKRGKEECFKGNVFQIVLSRRFYQ
                     QYQGDDFMLYRTLRSINPSPYLFYFDFADFRIFGSSPEAHLVVDAKAQKAYIKPIAGT
                     FRRTGDDIKDLELAEKLRTDEKENAEHVMLVDLARNDLSRNADEVKVETFREVQYYSH
                     VIHLVSCVSGKLRPGTKIMKFFADTFPAGTLSGAPKIKAMQLIDQIEPHDRGPYGGCI
                     GYIGFDGGFNQAITIRSFISKNNTLFYQAGAGIVAKSDEENELQEVNNKLAALKQAII
                     LAGKRNNHQTE"
     assembly_gap    18732..18831
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18832..19937)
                     /gene="trpB"
                     /locus_tag="PLD44_04605"
     CDS             complement(<18832..19937)
                     /gene="trpB"
                     /locus_tag="PLD44_04605"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOF98648.1"
                     /translation="MPENSQKSRNGADRKLYQPDEKGFYGEFGGAYIPEILYASVEQL
                     KKAFLSIKGDENFKNEYYDLLKNYVGRPTPLYFARRLSELYGVNIYLKREDLNHTGAH
                     KINNALGQVLLARKMGKTHMLAETGAGQHGVAAATACALMGMDCTVFMGKTDIERQYL
                     NVQKMRMLGARVEPVTSGNMTLKDATNEAIRYWCSHPDSYYIIGSTVGPDPYPEMVGY
                     FQSVISEEIKWQLQEKIGRNYPDYLMACIGGGSNAMGTYFHFLDDERVNIISAEAGGM
                     GIETGKTAATIHLGKTGIIHGAKTLILQNEDGQIDEPYSVSAGLDYPGVGPAHAFFTK
                     IGRTKVYAINDDEAMEAAFELTRLEGIIPAIESA"
BASE COUNT         6120 a         3866 c         4112 g         6088 t
ORIGIN      
        1 gacagtgatg gagatatctg gatttcggtc gaagaaaagg gagtttattt gtatcattat
       61 gagaataata cactggatca tttcccggtt aaaacgcctc ccggaggatt gaaaggaatt
      121 aaactctgcg aaaacaaagg aaaaggaatt tgggcttttc cttattccat gccgtttctg
      181 caaattgaca aaaaaagccg taaaatccaa ccgttttctt tgaatcaggg caatgaaatt
      241 ctttataata cgggtgaaat cggaagcgta attgaagaac aaaacaattt gctggtaggt
      301 acttccttgc agggactttt acaaattaat ctggtagaac gaacttacaa caaattattg
      361 gcttcggacg aaaccggaca gccgattttt gtgcgaaata tcttgcgaaa aggggatgat
      421 ttgtgggtgg caacggaatc gggaatttat atttacaatc tttattcccg tgatgtcatt
      481 aatttaaggc acaataacgt tattccctat tcactttcgg acaatgctgt ctattccctt
      541 tatgaagaca atgaaggcgg cgtatggatg ggaacttatt ttggcggagt aaattatttc
      601 cccaaccgac tgacttattt cgaaattttt tatccacttg aaaatgaaaa ttcaataaag
      661 ggaagaaggg ttagagaatt ttgcgaaaac acggatggac gattgtggat tggaacggag
      721 gacaacggat tgaatttgtt cgatcctgaa acttcaaaat tccttcccgt cgacagtaaa
      781 ttaaactcct tataccacaa tatccatgca cttttcagag atggaaacac cctttggatc
      841 ggaacttttt ccaaaggttt gaatcggtat gatttgaaaa caaaaacact caaaacgtat
      901 tacaaaacga gtgagccaaa ttctttgtcg gaaaacagcg ttttttccat ttgcaaagat
      961 cgacagaata ccttgtggat aggaacttta tcgggattga atattttcaa ttaccaaaac
     1021 gattcattta cccgaattaa ggaattggaa ggtgcttttg tccaggatat ttttgaagat
     1081 acggatggaa atatctgggt tgctactttt gtaaaaggtt tgttcctgtt taatcccaaa
     1141 actcacacat ggaaaaactt cgtacacact tccaatgccg gcagtcttcc ttacaacaaa
     1201 acgacttcag tgtttgaaga cagcaaaagg agaatttgga taacaactga aggcggtggt
     1261 ttttgcaggt acgacaaaaa aaatcaatct ttcgtaactg tcaacagcac caacggttta
     1321 cccaatgatg tggtttatca aatacaggaa gacaattcgg gcaatttgtg gctttccacc
     1381 aattccggtt tggtaaaata tgtccctgat acgggtctaa tggaaaattt tacggtaaat
     1441 gacggcctta aaaccaatca gttcaattac aaatcgagtt acaaagctcc ggacggaacg
     1501 ctttattttg gttcgattga tggttttgtt cgtttcaatc ctgcttattt taaggaaaac
     1561 aacctcttgc ctcccattgt tttaaccgat ttctatttaa ataattcgcc cgccaatgtt
     1621 tcggatgcag gttcaccttt gaaagaaaac atttcgtttg cagaaaaaat tcggcttccc
     1681 tacaataaaa attcttttac cattaaatat gccattttga actattccgg tttacgccct
     1741 aaaaatgttg tttacaaact ggaaggattc gacaggcaat ggattccggc caatggttct
     1801 caatcggttg tttattccaa tttgaatccc ggaaaatatc ggttgatgat ctctttcgat
     1861 acaatttcct caggtggcat gaacaaagtc agtaaaacat tggatattgt tattaaacca
     1921 cccttttggg aaacatggtg ggcctatttg ctgtattttc tcatcactat tgccggaatt
     1981 tttgagttga tcaattattt tcgtcagcgg acaaagagaa ttcatctgga aaaaatgcga
     2041 gatttcgagc aggaaaagga aaaagaactc tatgaatcga aaatagattt tttcaccaat
     2101 gtagcgcacg aaatcaggac tccattgtcc ttaattaaag ctcctttgga tcaaattttg
     2161 cggaacgatg aaaccgttac tcccgatgtg aaggacaatt tgaaagtaat gagtaaaaac
     2221 accgatcggt tgctggattt aaccaatcag ttactcgatt tcaggaaaac ggaagccgaa
     2281 ctgtacaaat tgaaagaaaa acgcaccaat ttgtcggaat tgatgaaaga aacctttgac
     2341 cgtttcaccc cgcttgcaaa acaaaaaaat atttcattcc atttaaacct tcctgaaaat
     2401 gaagttttta caaaaattga tgcggaagcg ttcacaaaaa ttattagcaa tctgatcaac
     2461 aatgccgtga aatattgcga cagtatggtg aacatttcgc tcgaagtagt ggctgaaaat
     2521 catcccgaaa aactggtatt taaaacggaa aacgacggag cggttattcc ggaacgtttt
     2581 aaaaatgaaa tatttaagcc atttacacga ttgaataagg aaaaagataa aatagttacc
     2641 ggaaccggca ttggtttggc tttgaccaag tcgttaacgg aacttttaaa gggcgacatt
     2701 acttacaaag ttgaaaatgg tttgaacgtg tttcgggtta ctctaccatt taatccttta
     2761 accggcaacg agtggattga agaaaagacc gaaatgcagg aaacgcccag tagaaaagag
     2821 gaagataaag ttgaaaaatc gttaaaacgg ccgttaattc ttttggttga agatgataaa
     2881 gggatgatgg aatttttgac aaaatgtttg aagagggaat acagtgttct ttctgcttcg
     2941 gatggtaaag aagctttgga ggttttgaaa aataaaaata tcgaccttgt tgtcagtgat
     3001 gtaatgatgc ccgagatgga tggttttgaa ttgacatcct atatcaaatc gcatatcgaa
     3061 tacaatcata ttccggtaat tcttttaacg gccaaagtaa acgttcaatc caaagtgcaa
     3121 ggttttgaag tgggagccga tgcctatatc gaaaaacctt tttcggtgga cgttttactg
     3181 gctcaaatag caagtttgtt gcaaaacagg gagaaatttc gcgaagcttt cttgaaaaat
     3241 ccttttaagg gactcaatca cgttgagttg aacaaatccg atgaagaatt tttgcagaaa
     3301 ctggacgaca ttattcagga aaacatatcg aatcctgatt ttaacattga agatttgtcg
     3361 gaacatttct ttatgagccg cgccagcttt tacaggaaaa taaaaggaat cctcgatttg
     3421 actcccaatg aatatcttcg ggtggcaagg cttaaaaaag cagcgcaact cttgaaggaa
     3481 ggcaaccaac gaataacgga aatttgttac ttggttggat ttaattctcc ttcttatttt
     3541 gcgaagtgtt ttcagcaaca atttggcgtt ttgcccaaag atttttcgta aattctgcta
     3601 ttttgagatg atatttttat ttttcgattc atttttttct tttgaatatt gaattttaag
     3661 ctaagtttgc ttgacaaaga atactaaaaa tttagccaat gttgagaata aaattattat
     3721 tgttaagttc actgtttagt gtcattactt ttgcccaatg gcagccagtc ggagaaaaaa
     3781 ttaaaacacg ctggtcgtcc cagattgatg tcaataatgt attgcccgaa tatcctcggc
     3841 ctttgatgga acgttcggaa tggatgaatc tcaatggttt atggaattat gccattcttc
     3901 cggttgggaa accagctcct tccgcttttg atggtaaaat tctggttcca tttgctgttg
     3961 aatcaagttt gtcgggtgtt cagaaaaatg taggaaaggc aaacgaatta tggtacgaac
     4021 gtgaattcac cataccttca aaatggaaaa ataatcgtgt acttctccac ttcggggctg
     4081 tggactggaa aaccgacgtt tgggtaaacg atgttaaggt tggtcggcat acgggaggat
     4141 atactccttt ttcatttgaa attactactg cactaaaaaa cggttcaaac aaattgatag
     4201 ttaaggtttg ggatccaacc aatgatggtt atcagccacg tgggaagcag gtgaataaac
     4261 ccgaaggcat ttggtacacg ccagtaaccg gcatttggca aacagtttgg ctcgaacccg
     4321 ttccggataa atttattact caggtaaaaa caacaccaaa tattgatata aatattttga
     4381 atattgaagt tgctacaaac aatttttctg cttcggacag aattgaagta aaagttatgg
     4441 atggctcaaa agttgtggct acggctcagt ccatcaatca tcttcccgta gaaattgtta
     4501 tgcctgatca agtaaaactt tggtcgcccg acagtccgtt tttgtacgat ttggaaattt
     4561 cgctttggag tggaacaaaa caaatcgata aagtcaaaag ttatgctgcc atgcgaaaat
     4621 attctatcaa acgggatgaa aatggaattg ttcgtttaca gctgaataat aaaaatttgt
     4681 ttcaatttgg gccgctggat caaggttggt ggccggatgg cttatacaca gctccaacag
     4741 atgaagcatt aaaatttgat attcagaaaa ccaaagattt tggttttaac atgattcgca
     4801 aacatgtaaa agttgaaccc gcgcgctggt acacttattg cgaccgtctg ggcatgattg
     4861 tttggcaaga tatgccaagt ggtgatggtg gcccgcaatg gcaacctcat cagtatttca
     4921 gcggtttgga atggcttcgt tcgcccgaat ccgaagcaaa tttccgcaag gaatggaaag
     4981 aaataattga ttttctgtat tccaatccgt gtataggcgt ttgggtgcct ttcaatgaag
     5041 catggggaca atttaaaacc aaagaaattg tagaatggac caaagagtat gatccttcac
     5101 gattggtaaa tccagccagt ggtggcaatc attatccggt tggcgatatg ctggatcttc
     5161 acaattatcc taatcctgca ttgtatctgt atgatgcaca gcgtgctaca gttttgggag
     5221 aatatggcgg aattggctgg gctgtgaagg gacatttatg ggaacctgat cgcaactggg
     5281 gatacgttca gtttaacagt gccaaagaag ttaccgatga atatgtgaaa tatgccgaac
     5341 aactgatgaa tttgattcag catggttttt ccgcagccgt ttatacccag actactgatg
     5401 ttgaagtgga ggtgaatggt ttgatgactt acgatcgtga aataataaaa atggatgaat
     5461 cgagagtcaa acagataaat caaaaaatat gcaatatttt aaagaattag ccatttgtat
     5521 atctgtttta ctttgttttt cgtgtcagaa aaacaacgaa attgcagcga aatcaccttc
     5581 actgtctatt cgatgggatc aaagttcgtt ggtgtgtatc gctgccgaag gcggatatcc
     5641 gcgtgtaaaa agattgaacg acggttcgct tttggctgcc tacgaaaaca gaaaagggga
     5701 tgtgttggta aaacaaagtc gggatgaagg aaaaacatgg agcgattcgg tgctggtttt
     5761 cgcaagtttc gattatattc atccttcaaa tggactttcg gcaaaaatca atatcgccaa
     5821 tccggaattt atccagcttg ggaatgacga tattctgttt gcctgcaact ttcgtcccaa
     5881 taaggatgaa gtttaccctt tttctattgc cgtaaaaaga agtcgagata acggaaaatc
     5941 gtggagcgaa cagcaaattg tttatcaggc agctccgcga ttcattgacg gatgttggga
     6001 accttctttt cttcttttgc cggacggcag aattcaactc tattttgcca atgaattccc
     6061 ctatcagcaa agcaacgaac aggaaatttc catgctcgaa tccaatgata atgggacaag
     6121 ctggtcggcc gataaaaaga ctgtgagctt ccgcaaggga tttcgtgatg gcatgccggt
     6181 tgcaacgacc aatggaaatg aaattttcgt agctatagaa gacaatggct ttgatcaatt
     6241 caaacctttt attgtgaaaa atgccattgc tgataattgg aaagaacctg ttttagaaga
     6301 atcccccaat cgctattcag ctttgaaaga acctcttcct tcttcagttt atgcgggtgc
     6361 accttatttg attcgcacaa aagaaggata ttacgttttg tcgtatcaaa cgacccatca
     6421 tcgtacttcc aactgggaat tgtccacaat ggaagttgtt gtcagcgata aaccgtccga
     6481 ttttatttca ccatcgcaac ctttcgctgt acctttaagc aaggaagcca aatggaattc
     6541 gctgtgcgat ttgggaaatg gcgaaattgc tgctgtttct tcaaccaatt tcaaatcgga
     6601 taaaatcggt atttggatga ttaaaggaaa aattgtagat aatcgaaaat aaaatttatg
     6661 aaacgttcgc ttggaataat attttttaca ctgcttttct tttcatgcaa gggaagtgag
     6721 gcaatcattc ccgaaacaga gagtgttagc acttacaaaa atcccatcat tccacgaagt
     6781 ttgcctgacc caacggtaat aaaagcttcg gatggcaatt tttatcttta tgcaacagaa
     6841 gatatccgta atgttccgat ttttcgctcg tccgatttgg tgaactggaa ttttttggga
     6901 accgctttta ccgaccaaac gcgaccgaca tttgtttcgg gtggtggtat ttgggctccg
     6961 gatatcaatt atgtcaatgg aaaatatgtg ctttattact ccatgtctgt ttggggtggc
     7021 gaatggagtt gtggcattgg agttgcaaca gccgatcatc ctgaaggccc gtttaccgat
     7081 cacggaaagc ttttcattag tgatgaaatt ggggtgcaaa attccattga tccttgttat
     7141 ttcgaagaaa acggcaagaa atatctgttt tggggaagtt ttcgaggaat ttatggtatt
     7201 gaactttccg atgatggatt gtcggtaaaa ccgggagcac agaaagtgca gatggcagga
     7261 actgcttatg aaggaacata cattcacaaa cgaaatggat tttattattt gtttgcttca
     7321 atcggaagtt gctgcgaagg attaaacagc acttacacag cagtagttgg acgttctgat
     7381 aacctttttg gaccttattt agataaatca ggacaatcca tgatggaaaa tcaccacgaa
     7441 attttgattc agaaaaacag caaatttgtc ggaaccgggc ataattcgga aattgtttcg
     7501 gacgataaag gacaagattg gatattttat catgccgttt gggtggacca tccacaagga
     7561 agagtgttaa tgctggataa agttcaatgg atgaatgatt ggccttatgt aaccgataaa
     7621 tgtccgtcat tagaagctga aaaaccgtat ttcaatgaat aattgttcaa taaaaaagtt
     7681 ctatatattg atccgataaa acaaaaaagt catgagcaag aaattgaata tcgttttttt
     7741 aatgttggta ttgctgcttc cgttgcaggg ggcaaaaaca gtttcgacca aacagtcagt
     7801 ttggacaaaa gaaaaagcca atgcatggta tgcttcgcag ccgtggttga gcggttgcaa
     7861 ttatatccct gcgtcagcca tcaatcaggt agaaatgtgg gcaaaggaaa catacgatgc
     7921 accaacgatt gacaaagaat tgggttgggc tgaagaactt ggattcaaca ccatgcgtgt
     7981 ttttttgagt agtgaagtct ggaaagacga tccttcggga ttgaaaaaac gaatggacaa
     8041 cttcctttcc atttgcgaga aacaccacat tcgtcccatg tttgtatttt tcgacgattg
     8101 ctggaatgaa gaaacagtta aaggaaaaca accggcaccc aaacccggca ttcataattc
     8161 agggtgggta caggatcctg cctgcagcct gagagccgac actgctacac tctttcctgt
     8221 actcgaaaaa tacataaaag atatcgtttc cactttcaaa aacgatcaac gtattctcat
     8281 gtgggatttg tacaacgaac ccggcaacag ccgacatgga attaattcgc tgccactgct
     8341 gaaaaacaca ttcaaatggg cacgtcaatc gaatccatcc caaccgcttt cagccgggat
     8401 ttggtatttt ggttgccccg aactcaatga atttcagttg gaaaattcgg atgtggtaac
     8461 ctatcacaat tattcggatg aaaaaaatca cgaattatgg atcaatttcc ttcggatgca
     8521 aggacgaccc atgatttgta ccgaatacat ggctcgacgg aacaacagtc atttcgaaaa
     8581 catcatgccg ttgctcaaac gcaataacgt aggagccatc aattggggtt ttgtttcggg
     8641 taaaaccaat acaatttttg cttggagcga tccgcgtccg aacgaaaaag aaccgaccgt
     8701 gtggttccac gatatttatc gtcaggacaa aacacctttc gacccaaagg aaattgaatt
     8761 tatcaaaaag atgaccggca aagagtccaa tgtgcagctg atgaaacccg aaaatttcaa
     8821 tgaaaaaatt gatggcaaac agatatcgct tttcaccttg aaaaatcgtc agggaatgac
     8881 agcccaaata acgaattacg gaggacgagt tgtgtctctt tgggtgccgg acaaaaaata
     8941 cgatttcaag gatgtgataa ccggatattc ctcgctgaaa ggattccaac aatccactga
     9001 agtttatttc ggagcattaa ttggaagata tggcaacaga attgccaaag gccgatttga
     9061 attgaatgga aagacttatc agctcccgat caacgatggg gaaaaccatt tgcacggtgg
     9121 acccaaagga tttcatacgg taatatggga tgcccgacca ttcaaaaatg ccaacaatga
     9181 agatgctttg gaactgaaat acttttctcg tgatggcgaa gaaggttatc cgggtaattt
     9241 gacggttaag gtggtttata cattgacaaa cgacaacgag ctgaagattg aatattctgc
     9301 cacgaccgac aagccaacca ttatcaatct gaccaatcac atgtttttta acttgcatgg
     9361 attttcggat ggaattgcga aatcgatcaa ttcacatgtg ctggaaataa atgcgtctaa
     9421 ttatacaccg acggacaaag gattgattcc aaccggaatc attgctccgg taaagggaac
     9481 gccaatggat tttacagtac caacgccaat aggagaaaga gtaaatgaaa actttatcga
     9541 tttaatcaac ggaaaaggat acgaccacaa ttgggtattg aataaaaaag gagataaact
     9601 gacagaagct gccgttgttc acgaaccttc caacggcatt caaatgcgag tgattaccga
     9661 ccaaccggga gttcagtttt acggaggcaa tttcttgaat ggaacagaaa gaggtaaata
     9721 tggcgaagtc tatacgtatc gcacttcatt tgctatggaa acccaacatt tccccgacag
     9781 tccgaatcac ccgaactttc cttctacggt tcttaatccg ggtcaacagt atcaacatgt
     9841 ttgcatttat aaatttgaag taggcggtaa ttaaaaacac tgcctacaaa agatgtgttt
     9901 tcttaattgg tttaaataac agttgtttcc tttaaaagat ggttaataat ccgatatttg
     9961 ttttccaatg aaaacttatg tttcaggaac ttattttctt gaattgaatt caaatggaaa
    10021 gatttaccat ctgactatca ttaaaaaagt aatgttccaa attctcaaaa ttcttaaaga
    10081 gccggcctaa acaaccggct ctttaatttt tcagacaaaa aacttgaaat tcttaaaatt
    10141 ttaatctggc agaggcaaga aaattggttc cggcctgtgg aaaatatctc agttcgttat
    10201 gccgttcgtt gcccagatag caggaatacc atgtgtaacc gttcgtttcg tatttttcgt
    10261 tgaagagatt attgatttga acgagaaatt cgagcgtttt gataggactg atagcaagcg
    10321 agtatttcaa attcaaattt tgaacaaaat atgggtcaat agaacgttga atatccgaag
    10381 tgttgtctat atactgtcga ctaacgtaat ttgaatgaaa agccgcttcg aaacgcttga
    10441 gatgaaaaga aaaaatactg ttagcaatca cgtttggaga ataggctata tcggttgtcc
    10501 caagttcgtt gttgcgcttt ccgatgtact tccagttttc gtcgtatacg taaatatcct
    10561 gttcagtaaa atttttgatt ttattgcgac taaaagtgca atttccgtcc cattttaaat
    10621 agttattaat tttccaacct gctgtcagtt caattcctgc ccgatagctg tcgggaatgt
    10681 tagtagtcag aaattctccg atttcactga tttttcccgt gagaatcaac tgatttttat
    10741 atttcatata gtaaaaattg gtgccggccg aaaatcggga cgatcggtaa cgatagccgg
    10801 cttcggtatc gtataatgtt tcgttggttg gacgttcgtt tgctccggct tcagtaaaat
    10861 tgttcctgtt tggttctctg tgagatacgg aataggaagc gtaaaattca tttcgggtac
    10921 ttggttgcca tgtgaatccg aatttcggat tgaaaaaatg gtaaatgtgc tgttgggtaa
    10981 tgtcccgcat ggattgattt attttatcat atttgtcatc ggttccgtcc atccgatagt
    11041 taatatatcg gtattgcaaa tcaattgaag caaacaagtt tttaaaagtt tccgtattca
    11101 acttggcata aatattggca tcgtctttta tggaactgct tctgtgccag tctttcccaa
    11161 tgtccaaatt agctgcattg cgaacccaaa ttatttttcc gtaatgttgt ccatcatatc
    11221 ggttcgcagc tccgccaagc gaaaagttcg tgtttttttt ctcgtaattc aaagctccgg
    11281 taatgccata gaaatagttg tcgagccatt tttgccgaac caaatcggtc gttttcagcg
    11341 tatcaaaacc tataatggca ggtgcgagcc catattctgc atatttcctg tcggttttat
    11401 actcttcata ataaccgatt cctcgagtca gatgggtagc tacatttgaa tggagatggc
    11461 tgttccattt ttgcaaccag tagagatgat aatgagtttg caaataattg tcggtttggt
    11521 tgtcgtagaa ttttaattct ccattgttgt cggtataggc gcccaaatcg ttgtatctcc
    11581 tgtttgtttt cattatttcg gcatccacgc cgttccatgc ctgataagtt ttttcttttc
    11641 ctccgaaggt gagcaatcgt aaaactgttt tatccgaaaa atagccgccg gaaaagtaat
    11701 aggattgcat atctaccgaa gcgcggtcga tataaccatc ggaagttacg ttcgagaagc
    11761 gagcatcaaa tgcaaaattg ttgttcaact tgcccgatcc aactttaatg gagttcttgt
    11821 ttgttgcaaa agagcccaaa gaggtgctga tgtcggcata cggttccaat gaaacacttt
    11881 cggtctgcat attgatgctg gcgccaaaag cagctgcgcc attggttgaa gtgccaacgc
    11941 cccgttgaac ctgtaccgat gaaagtgatg cggccagatc gggaaaatcg acgaaaaatg
    12001 ttccgtgtga ttcggcatca ttcaacggaa taccattaat agtaacattt gttcggtttg
    12061 catctgttcc tctgattcta aatcctgtgt aaccgacacc gtttccggca tcggaagtta
    12121 ctataaatga aggtgtcagt gccaaaagat aaggaatatc ctgaccgaga tttcggcttt
    12181 ctatttcctc tttttttatt tcactgtaag caatggccga gcgctcgttg gcacgcaacg
    12241 aagttaccgt aatttcacca atttcaatgg aaattcgatt catttgaatc gtgatttctt
    12301 tgtccggagt cgtttctacc cattgagttt cgtaacccaa ataggaaact tgcagtacat
    12361 aattatttct ttttaaattt tcaaaaagaa aagtaccatt tttatcggtt gttttaccga
    12421 ttccttcctt tttcaggtaa atatctgccc caaagagggg atgaccattt tcatccaaca
    12481 cttttccctt tatccagtgt tgaccaaagg acattaacgt ccaaaacagc aaaattgccg
    12541 taaatacaaa ttgtctcatc acaaaaaatt aattaatggt taatactttg ttatgagagc
    12601 ctaaaaaaac ggttgattga aaacttattc cctactcgga attacccgtg caggttcaaa
    12661 gggtatgatc tcagcccgaa attattaagg caccccattt taataaaaaa attaagttga
    12721 tgaagaaaaa ccgcggattt ctctttttca agttgcaaag ttacaaaatc tttcccaata
    12781 aaagtttaaa ttcaagaaat tgatattttt ttatcaaaaa attatacgga gggaaaaagt
    12841 agtttttaat tttttctatt caattgaata tcaataatat agttgaaaaa gttgtattga
    12901 tttgctgaaa tcaacaagca tgaaatacgg taaaaaaacg atagaaaaac tagaaagaaa
    12961 accaaaaaaa taagtaacta attagaaaac aactttttat attcgaaatt cgataaaagt
    13021 gcaaatgaca ggaaaaataa aaattctgtc cggtgaattt gcaccatttt gccggacaga
    13081 attttttttg gtgcaaagaa tttatttttc ttgcagtttg actcaaaagt cgaaattact
    13141 ccttcaattt ttttatcaga aattttacgg ctgactccac atccgaactt tcctgcaagg
    13201 ctttgatgaa agcacttccg ataattacac ctgaagagta acgcgaagcg gtttcgcggg
    13261 tcaatttgtt ggaaatgcca aagccaatga gtctgggatt tttcaaatgc atcgaattaa
    13321 tgcgattgaa ataatcaata tgatggtcaa atgaattttg agtgccggta acgccggcgg
    13381 aagaaaccat gtaaataaaa ccatgcgtgt gttcatcaat ttgatgaatc ctttcttctg
    13441 aggtttcggg ggtaatcaga aaaataaaat ccaagccgtt tttttcgact atttctctgt
    13501 attccgacag ataaacgtcc ataggcaaat cgggaataat catgccatcc actccgactt
    13561 ttttacattc gctgcagaaa ttctcaaaac caaactgcat aatcggattc agataaccca
    13621 tcattaccag tggaatgtgc accgaagcac gaatgttggc aagctgactg aaaagtttct
    13681 ttagattcat gccgtttttc aaagctatct ggctgctgtt ttgaataacg ggcccatcgg
    13741 ccagcggatc ggaaaaagga ataccgattt cggccatatc cacaccgtat ttttgtaaac
    13801 tctccagaat tggaactgtg tcattcggtt ttgggaagcc ggccgtaaaa taaaccgaga
    13861 gaatgttttc ttttttttgc gtgaataact gttgaattct attcataatg aattatttat
    13921 gtatttttct tgttaaagtg aacgataccg atcaataaaa ttgcttaaaa gttcgacatt
    13981 ttttaagccg ggcgatattt cgaaacggct gttgagatcg acgcctgcaa aagccggatg
    14041 ctgaatttta ttaatatctt ctaaatcttc aatcgaaatt ccgccactga gaagaaaagg
    14101 tgttttttct ttgtaatttt ccagcaactg ccagtcgaat ttctgcccgg ccccgccata
    14161 aatgggagtt tgtgcatcga aaaggaaata gtcgcaactt ttttcgtagc gtttcattac
    14221 cttaaaatta taagcttcgg caacaggaaa agccttgatg accagcaatc ccgtatcgtg
    14281 caattggttg cacattttca ccatttcact tccatgcaac tgtacgccgt ctaaatggta
    14341 tttgtaaacc gtagccagga tttgatcgag tttttcgttg acaaaaacac caattttacc
    14401 gatttcggga ggcaattcat tcaaaacgga aacatttaaa ggttcggcat aacgcggcga
    14461 tttgggataa aagataaaac ccatgaaatc gggacggaga cgggctacgg cacgaatatt
    14521 ctccgggaat ttcattccgc agacttttat caattttttc tttgtcgtat ctgcaaaccg
    14581 agacggaaca atgttgtttt tttctactga tctcataaag aaaattcaag taatgtaact
    14641 tttgcagtga ttaagaggtt taacaaaatt atttgtcagg aaaatattta tgaaacaaat
    14701 ttacaaatat atcgactaat cgagtcaata atctaaagct gctaattatc ttcattcaat
    14761 acttttattc atttaactgt ctcaaaaact ctttcagagc aagagccggt tggacttcct
    14821 tcataaaatt ttcacccatc agaaagcctc tgtatcccaa tcggttcaat tctttcacag
    14881 tttcgacatt ggaaatgcca ctttcactga tttttacaaa ttcaggagga ataaacgcag
    14941 ccaaatttgc tgaaatgccc gtgtccacct gaaaagtttt cagatttcga ttgtttacgc
    15001 caaccatatc cacattttcg ttcaaataac tcaattcttc cttattgtgc acttccagca
    15061 gaacttcaag tcccaattgg tgagccaacc gtgaaaaatt tttaacttca aaaacggtta
    15121 aagctgaggc aatcagaaga atcacatcgg cgccaatggc tttggcttca tagatttgaa
    15181 attcatccac cacaaagtct ttccgtaaaa tgggacagga aattttattg cgaacggtcg
    15241 ttacatcttc aatatttcca ccaaaaaatt cggtatcggt aaggatagaa attcctgaag
    15301 ctccttccac attgtagccg atagtaactt cctgcacatt ggcatcggca tgaatccaac
    15361 ctttggaagg agatttacgt ttgaattcgg caataattcc caaagaagaa ttttgtaatt
    15421 tctgtttaag cgaaatagtg cttcgatcaa aaaaattaga attttccaac tcgttaatcg
    15481 acacatttag ctttcgtttg ctaacttcct ttattttatt cagaagaata ttattcagga
    15541 aattttctcc tgggttcgat aagtgattgt tttttaatgt tgaatcagtc attggtagaa
    15601 atcataaatg atatatttct tcaaacttta caaatgcttt ttttgctttt ccgcaggcga
    15661 gcgatgcgat ggcttctgcc ttgcattctt cgagcgattt ttcggggcag cgcgtttgta
    15721 tggcaaatgc agaattaatg accactacat ttcgctgggc atcggaagct ttgttatcca
    15781 atacattcat aaatatttcg gctgcatgtt ccactgttgt gccaccccac aattcttcct
    15841 gctttactcg ctgaaagccg agttgttggg gactgtaaac atattcgcca aagttgttga
    15901 ttattttgca gtcgtcggta agtgaaactt catcatatcc gtcgagagtg taaacaatgg
    15961 tataatttgt tcccaaccgc tgataaatat agttgtacaa gcgcatcatt ttcaggttgt
    16021 aaactcccaa gcactgataa cgcggtcgaa tgggactgat caacgggcca agcacattga
    16081 agaaggtgcg aacgccgata tttttacgga cgggagcaac atttttcatt gccggattgc
    16141 agaatggagc atgcaaatag gcgaaaccgg attcttccag tgagcgccgt acaacatcag
    16201 tatcggtggt aaatttggcg ccgtaatatt ccagtacgtt ggaagaaccg ctgatggaag
    16261 ttgctccata atttccatgt ttggcaactt tgtaaccggc accggccaat acaaaacagc
    16321 tgcaggttga aatgttgaag gtgttttttt cgtcaccgcc cgttcccacg atatcaatgg
    16381 gatcgaaatc cgataaatcc attgggatag ccatcgacat caaggcatca cggaacccga
    16441 tcagttcgtc cagttcgatg ctgcgcatta gatataccga aataaaagcg gcaatttgcg
    16501 attcgttgta catgccggct ccaatatttc tcattacttc ggccgattct tctctggtca
    16561 acgactgatg attaaataat ctgtttaata tctgtttcat aagttaactg tttttttgct
    16621 gaaaatgagg aaaaaacttg ttttctctga tttgtttctc tataattttt taaatctatt
    16681 atttccccga taaagggaat tccaaccaat ttttcagtat ttgttcaccg gaaggtgtca
    16741 gcaccgattc gggatgaaac tgaacgccgc gaacatcaag ggtgcgatgt ttcagcgcca
    16801 tgatggtacc ctgctcgtcc tttgccgtaa cctgcagaca tggtggcagt gtagaatcgt
    16861 ttacaaccca ggaatggtat ctgccggcaa taattttttc cggcaaattt ctgaacaaaa
    16921 cttcatcgtc aaaaatgctg acttcggaag caatgccgtg ataaacctga ggaagattga
    16981 gcaagcttcc accgaaagct tcggcaattg cctgaacacc cagacacacg ccaaaaatgc
    17041 ttttcgatga agcataagtt tttattaaat cgagtaaaat gcctgattcg gaaggaacgc
    17101 ccggaccggg agaaagaata attttgtcga aacggtttat ttcttctaat gaaatccgat
    17161 cattgcgatg aacttccaca tcagtgaacc ccaacttttt aactatgtgt accaaattat
    17221 aggtaaagga atcgtaattg tcaaaaacga gcaatttcat accggttgta ttgttttttc
    17281 ggtctattta aagttttcgg agaaaattct gaaacttgtt cgataggcca tcggtttatt
    17341 aaaatctatt ctgtttgatg gttatttctt tttccggcca atataatggc ttgtttcagt
    17401 gcagccaact tattgtttac ctcctgcaat tcgttttctt catccgattt ggccacaatg
    17461 ccggcacctg cctgataaaa aagcgtgttg tttttgctga tgaaagagcg aatggtaatg
    17521 gcttggttga atccgccatc gaacccaata tagccaatgc aacctccata agggccgcgg
    17581 tcgtgtggtt caatttgatc gataagttgc atggctttaa tttttggagc gccggaaagt
    17641 gttccggcag gaaaagtatc ggcaaaaaat ttcatgattt ttgttccggg acgcagtttc
    17701 ccgctaacgc acgaaaccag atggataaca tgcgaataat attgcacttc ccgaaaagtt
    17761 tcaaccttca cttcgtcggc atttcggctc agatcgttgc gtgccaagtc caccagcatt
    17821 acatgttcgg cattttcctt ttcgtcggtt ctcagttttt cggccaactc aaggtcttta
    17881 atgtcgtctc cggtgcggcg gaaagttccg gcaataggct ttatgtaggc tttttgtgct
    17941 ttggcatcga ctaccaaatg ggcttcgggc gaagaaccaa aaatgcggaa atcggcaaaa
    18001 tcgaaataaa aaagataggg agaaggattg atagaacgta aggtacgata aagcatgaaa
    18061 tcgtctccct gatattgctg gtagaaacgt cttgaaagga ctatttgaaa aacatttccc
    18121 ttgaaacact cttcctttcc gcgttttacc atccgtcggt agtcctcgtc gttaatatcg
    18181 catttttcgc tgccaattcc cgaaaattcg taggcggcaa tattgttgtt gaggagtatt
    18241 tcttctattt cgttcatggc cgacttttcg ttctccagca aattttcaat gattgtcaac
    18301 tcatttttaa agtggttgac ggcaatgata tatttgaaca gcacataatg caaacccggc
    18361 atacttccgg gaacagtttc gcgtgccgaa agccggatgt cttcaaaata ctgcaccgct
    18421 tcataagaag tatagccaaa aagcccgttt actacggcgg gaacttcgtt gtcctgttcg
    18481 atacgttcga atgatttcag aaaaatatca aaacggtcgg ctactgtcat cttgtccgtt
    18541 acgggagttt gaatttttgt accatcgggg taatattcat gtacaaggaa atgattgacg
    18601 gaaattcctc caatgggaga aaacgcaatg aaagaataac tgttttcggc cgcatgataa
    18661 tcggaacttt ccaacaaaac cgaatgagtg tataaatctc tgatttttag ataaatgcca
    18721 accggcgttt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgagccgat
    18841 tcgatagccg gaataattcc ttccagacga gtcagttcaa aggctgcttc catagcttca
    18901 tcatcgttga tggcataaac tttggtacga ccaatttttg taaaaaaagc gtgtgcaggt
    18961 ccaacgccgg gataatccaa accggcagaa acggaataag gttcgtcaat ctgtccgtct
    19021 tcattctgca aaatcaaggt ttttgctccg tgaataatgc cggttttccc caagtgaatg
    19081 gtagcagccg tttttccggt ctcaattccc attccgccgg cttcggccga aatgatattg
    19141 accctttcat catccagaaa atgaaaataa gttcccattg cgttgcttcc tccgcctatg
    19201 caggccatca aataatcggg ataatttcgt ccaatttttt cctgcagttg ccatttaatt
    19261 tcttcgctga taaccgattg aaaatagcca accatttcag gatacggatc cggaccgact
    19321 gtagatccga taatgtaata agaatcggga tgcgagcacc agtaacgaat ggcttcgttg
    19381 gttgcatctt ttaaggtcat gtttccgctg gtaacaggtt ctactcttgc acccagcatt
    19441 cgcattttct gcacgttcag atattgacgt tcgatatcgg tttttcccat aaaaacggta
    19501 caatccattc ccatcaaagc gcaagcagtt gcagcagcca caccgtgttg tccggctccg
    19561 gtttcggcca gcatgtgcgt tttacccatt tttcgggcca atagtacttg tcccagtgca
    19621 ttgttgattt tatgtgcacc ggtatggttc aagtcctcac gtttcaagta aatattgaca
    19681 ccatataatt cggaaagtcg ccgggcaaaa tacagaggtg ttggtctgcc aacatagttt
    19741 ttgagtaaat cgtaatactc atttttaaag ttttcatccc cttttatgga taaaaaggct
    19801 tttttcaact gctcgacgga agcatataga atttcaggaa tatacgctcc gccaaattca
    19861 ccataaaaac ctttttcatc tggctggtaa agttttctat cagctccgtt tctcgatttt
    19921 tgactatttt cgggcatagt ttaattgttt ttttgattgt atttttatca atgaaaaaag
    19981 gcttgtcgtg agttcccgac aagccttttc ataaatattt ctttatttca actatcaaca
    20041 taggtgtccc ttctcacgat ctgaatattc gcaagcgcca ccaccaaaat ttgttgatta
    20101 ttgaatttgt tttcattttt tacagtctgt tttgttttcg gttgcaaagt tgataaatta
    20161 ttttgaattg tgcaacaaaa aaatcaattt ttaattcaga aaattattaa tatccaactt
    20221 acaagtattt tgctcaataa atcatcctca tcttacttgc aaaaagtgta atttaatatt
    20281 ttgaat
//
LOCUS       DAOSIB010000034        19829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_2984_length_19829_cov_11.087033, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000034 DAOSIB010000000
VERSION     DAOSIB010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19829
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2984_length_19829_cov_11.087033"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..2430
                     /gene="recQ"
                     /locus_tag="PLD44_04610"
     CDS             247..2430
                     /gene="recQ"
                     /locus_tag="PLD44_04610"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOF98649.1"
                     /translation="MLKKVSLAKELKKYFGFDTFKGNQEQIIQNVLNGNDTFVLMPTG
                     GGKSLCYQLPSLLMDGTAIVISPLIALMKNQVDAMRNFSEEDGVAHFINSSLSKQAIE
                     QVKCDILSGKTKLLYVAPESLTKEENIEFLKHIKISFYAIDEAHCISEWGHDFRPEYR
                     RIRPIVNEIGIAPIIALTATATPKVQHDIQKNLGMLDAKVFKSSFNRPNLYYEVRQKT
                     NNIDKDIIKFVRSQEGKSGIIYCLSRKKVEELAEMLHVNGISALPYHAGMDAAQRSEN
                     QDKFLMEEASVIVATIAFGMGIDKPDVRYVIHYDMPKSLEGYYQETGRSGRDGGEGQC
                     IAFYSYKDLEKLRKFMQGKPLAEQEIGNQLLIETQAYAESSICRRKLLLHYFGEEYKE
                     DNCGNCDNCMKPQKFMEAQELLTLVLETISILKEKFKAEHIVDILKGNETSDVVSYQH
                     NELENFGAASDEDEKLLQAVIRQALISGYITKDVETYGVLKITPKGKDYLKNPTSFPI
                     VKDNDFEEDEEVNLKNIASTSAADEVLFSILKDLRKKLSKKLDVPPFVIFQDPSLEAM
                     ATTYPITMDELQNIPGVGAGKAKRYGDEFLKVIKEYVKENDIIRPEDLRVRTVANKSK
                     LKVSIIQAIDRKIALDDIALSKGLDITELLDEIEAIVYSGTKINIDYFLEDVMDADRV
                     DEIFEYFNSAETDSIDDALDKLGIDDFSETEIRLVRIKFLSEVGN"
     gene            complement(2501..3373)
                     /locus_tag="PLD44_04615"
     CDS             complement(2501..3373)
                     /locus_tag="PLD44_04615"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOF98650.1"
                     /translation="MKVAIFGNIYRPVIINSIKVLFDFFSEKQIPLMLDSDLYDFLVH
                     IGGYRLENVEKIENDNFKADVALSIGGDGTFLNTAARIGDKEIPIIGINTGRLGFLTD
                     VSNDEIIEALEALLEGKFRIEERTLLLVKTSDGTPLQYPYALNEVSVLKQDSSSMISI
                     NASVNGELIHTYQADGLLVSTPTGSTAYSMSVGGPIVVPQAQNFILSPVASHSLNVRP
                     LIIPDNWNIDLEVHSRTHSYLVALDGRSKVLEQTTQLHIAKAPFTIKVIKQLQHTFFD
                     TLKKKLMWGVDKRN"
     gene            3785..4498
                     /locus_tag="PLD44_04620"
     CDS             3785..4498
                     /locus_tag="PLD44_04620"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOF98651.1"
                     /translation="MTKLSVNINKIATLRNARGGNTPDVCKAAIDCEKFGADGITVHP
                     RPDERHIRYSDVWALKPILQTEFNIEGYPSPNFMKLVTMVKPTQVTLVPDPPEAITSN
                     AGWDTEKHLDFLKDITAELQDAGIRVSIFVDANIKSVEYAALAGADRVELYTEPYARE
                     YAENREKAVAPFIEAAKAAKKLGLGLNAGHDLNLQNLHYLHACIPWLDEVSIGHALIC
                     DALYLGLQETIKQYKNQLK"
     gene            4549..5241
                     /locus_tag="PLD44_04625"
     CDS             4549..5241
                     /locus_tag="PLD44_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOF98652.1"
                     /translation="MINLLILQASANVATTVADPLQSEISLWTMAKFGGPIMIILALM
                     LAFAVYLFIERLITINQASKEDAFFMERIKDYIYEGKIETALKLCRDTNSPSARMIEK
                     GITRLGHPLNDVLVAIENVGNMEIAKLEKGLVIMASISGGAPMLGFLGTVTGMVSTFF
                     NLSQKAGGNILLGDLSVGMYQAMVTTIGGLIVGILAYFAYNYLVSRVDSVVNILESKT
                     MEFLDLLNEPVK"
     gene            5263..5679
                     /locus_tag="PLD44_04630"
     CDS             5263..5679
                     /locus_tag="PLD44_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOF98653.1"
                     /translation="MALKKRNKVEAGFAMSSMTDIIFLLLLFFVIASTLSSPNDIKLN
                     LPESRSKSSTKQIIAKVAIDAEGKLSVAKGNQKPVPIEPKLLESYLLNMASQDSISFV
                     ALYADQNIAYKEIVRVLDIANQHGFKIVIATKPFEN"
     gene            5686..6378
                     /locus_tag="PLD44_04635"
     CDS             5686..6378
                     /locus_tag="PLD44_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOF98654.1"
                     /translation="MKKSELYGIVGSIIFCMMLLVLLLIIKMHASTSVEPEGIMVSFG
                     ETLEGGSRAASPSAAEEYAVSKKASEKILTQKDPSVSLDKKTDNREQERELSEKKRLE
                     QEAISKANIMEGIFDKKISGNENVGDENLLKGNPAGRGTSNGNSWSLSGRNLVGNLIT
                     PRYEQNVEGEVTVNIRVDENGVVEGVSIGVPTNISDATILKAAMEAAKNTRFSPGKGK
                     ATGTITYHFRLK"
     gene            6457..7002
                     /locus_tag="PLD44_04640"
     CDS             6457..7002
                     /locus_tag="PLD44_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOF98655.1"
                     /translation="MKAMVFLADGFEEIEAIAPIDIMRRAGIEVTTISVSREKEVRGA
                     HGVLVEADRLFEEVSFSDADLLFLPGGMPGTKNLNAHDGLKQLLLKHANEGKKLAAIC
                     AAPSILGELGLLKGKEAVCFPGFEETLKGAIVSDKKVVQSGNIITGKAAGAAIEFALK
                     LVENLKGKSAAEQVGLSIFAF"
     gene            complement(7099..7371)
                     /locus_tag="PLD44_04645"
     CDS             complement(7099..7371)
                     /locus_tag="PLD44_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOF98656.1"
                     /translation="MNKAELISAVAEKSGLSKADAKKAIDAVVKSISEALIAGDKVSL
                     VGFGTFSVAERASRSGINPATKKPITIPAKKVAKFKAGAELSDAIN"
     gene            7663..8451
                     /locus_tag="PLD44_04650"
     CDS             7663..8451
                     /locus_tag="PLD44_04650"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOF98657.1"
                     /translation="MSYRSSFFGSIPVVVKNLILINIILWLATLVLPRFFLRWGINVD
                     LVNILGMHYWGSKQFNPAQLITYMFLHGNFEHIFFNMFALFMFGTVLEQFWGPKRFML
                     YYMVTGIGAALIQQLFWSVEYQPVISALNEAINTGNKDALFSHEGVLSRFFRINNLAA
                     LDTTSLIELKHLFVNLPVTIGASGAIFGLLLAFGWLFPDAKLMLIFLPIPIRARIFVL
                     MYAVAELFLGVAGFTGDNVAHFAHLGGMLFGIFLILYWKKKGVH"
     gene            8528..9421
                     /locus_tag="PLD44_04655"
     CDS             8528..9421
                     /locus_tag="PLD44_04655"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOF98658.1"
                     /translation="MNIFDNIKLSYRQGSHLIKLIYINLGVFLLYHAVLITFKLFNAS
                     GIFLLPFLAMPADGRQLLYHFWTPVTYMFMHEGFLHLFFNMLCLYWFGKIFLMYYSEK
                     QLTGLYLVGGWVAGLFYFLSFNIFPYFDAAVSQGLLLGASGSIMAIIVASAVASPNLE
                     MRILFLGGIKLKYIALVAILTSFFGITSNNAGGEIAHLGGALMGYLFVVSLRKGKDIT
                     KWLNGVLDNIVNLFSRKKLKIKKPKYSTSYRKMTDEEFNMEKSRRMQEIDRILDKIKT
                     SGYDSLTAEEKKRLFEQGKPR"
     gene            9488..10591
                     /locus_tag="PLD44_04660"
     CDS             9488..10591
                     /locus_tag="PLD44_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOF98659.1"
                     /translation="MILKKITKWGLISANIIVACALLFSVFAGFVSPAVFTFFAYFAL
                     VFPIIFFLNFAFFLLWIVLKKWYFLISLSLMLVSFPQLKTNFSFHLKKQPVSSIQPDF
                     TIISYNTHMMGLEKKHDEANKKINPVIQYVLSTDADIVCLQEFMVSTNEKYLTEEDAM
                     KAFSKYPYHHIYFKAHEKKSLLGIATFSKFPIINRQTIPYNSSANASIYSDINIHGKT
                     IRVINNHLESNQLTEKDKAMPYQLKDNFNTDLATNITVYFSKKLGAAYKLRAIQADEV
                     KKVISSSPHPVVVCGDFNDVPSSYVYTKIRGKLKDCFLEQGKGFGWTFNEKHYRFRID
                     YIFHDPSLVLLNFKKDKVPYSDHFPLKAAFQKL"
     gene            10808..11416
                     /gene="udk"
                     /locus_tag="PLD44_04665"
     CDS             10808..11416
                     /gene="udk"
                     /locus_tag="PLD44_04665"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOF98660.1"
                     /translation="MLTIGIAGGTGSGKTTVVRKIIERLPAGEVVVLPQDSYYRDSSF
                     LPLEKRLEINFDHPDSIEFELMIKHLQDLKEGKPIEQPIYSYLTCTRAAETVTIYPRE
                     VIIVEGILVLCNPELRELMDLKVFVDADPDDRLIRVINRDIEERGRTVNKVIERYEHT
                     VKPMHELFIEPTKRFADLIIPQGGDNHIAIDILTKYIENNLK"
     gene            11438..12175
                     /locus_tag="PLD44_04670"
     CDS             11438..12175
                     /locus_tag="PLD44_04670"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOF98661.1"
                     /translation="MTNADLQKIMFFDIETVPQTSSFSELSNEMAQLWEEKFQKLRIR
                     MPEKYKEDTSASEAFDTSAGIYSEFGKIVCISVGFIYFQGETMYFRTKSFASDDEKEL
                     LKNFSDLISKFCSSKDNMLCGHNIKEFDIPYICRRLLINGLELPPVFQISGRKPWENN
                     FLDTLELWKFGDYKNYTPLKLLTAVFGIPTPKDDIDGSQVAQVYYKEKDLERIANYCT
                     KDVIATAQVFLRLNGFELISPQNIENL"
     gene            12186..13565
                     /locus_tag="PLD44_04675"
     CDS             12186..13565
                     /locus_tag="PLD44_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOF98662.1"
                     /translation="MQRNIIFIVYLLIIIFLTSCREGEKVKLSSENSFRDIQEIVKSD
                     TLRAGTMYGSTSYFLFREEWMGYDYEMVKNFADYLKVNLKVIPAATESELFQLLKEGK
                     IDIAAYNLPVTKALKKHFLYVFPQPQTYMVLVQNMGRKALSSVTDLIGKEVYVKKNTL
                     FYQRLNALNKEIGGGIKIVIAPDSVSNDELIEMVANNKIDYTIAYYDKALLHKSYYKR
                     LDCRLPVSFEQPNGWLIRKENTDLFKTLSTWGQLAGTQRTEDRLINLYWEKSPYFITR
                     KIKIPKGSISPYDEMFKKYAPTIGWDWRMLAAIAFHESKFDSAEISRAGAVGLMQLMP
                     QTAASFGLNHENWFNPEANLDAAVQYIKSLNLIFREIKDKDERIKFILASYNSGPSHV
                     LDAMALAKKYGKNPHIWFNHVETYLLKKTDPDYYRDPVVKYGFFRGAETVRYVQNVLD
                     TYEKYLSRK"
     gene            13761..16829
                     /locus_tag="PLD44_04680"
     CDS             13761..16829
                     /locus_tag="PLD44_04680"
                     /inference="COORDINATES: protein motif:HMM:NF020246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98663.1"
                     /translation="MKKLIVKPLIIAFQIILILLPTDAKAQYFGRNKPSYQTFKYQIK
                     QTPHFEIYHYLTNDTVIKYFAGWTEEWYRFHQLIFKDTFKTRNPLILYANHADFQQTN
                     AISSIISEGTGGVTESLKNRVILPFAPTLYQTDHVLGHELVHAFQYHKLLKPDSAKYY
                     NINNLPLWMVEGMAEYLSIGSVDANTSMWMRDALIQKKFPSINDLNNTSEYFPYRYGQ
                     ALWAFIGKTWGDSIILPLFEKTAQYGLDRAIDSLFHFNEKTLSGMWKTATENHYGKLM
                     KDTTYNVIGKQIISKKNSGSTNVSPSLSPDGKYIAFFSEKNVFTLDLYLADAQNGKII
                     KKLWSVNRNQEIDDFSFIESGGTWSPDSKQFAFVVYSKGRQKLAIVDVRKAKMKLLEI
                     KGLNSFSNPEWSPDGRYIVVTGLVNGIGDLYLYEVKSGKTEKLTHDLFSNIHASWSSD
                     GKKIVYSSEYLCGAGSKKFAFHINILDLETREIKTLDVFPKADNLNPRFSNNNQYIYF
                     VSDADGFRNLYKYDLNTDKVFRLTQYMTGISGITTFSPAISTDRKNDLIAYTYYFNGN
                     YEIYTASATEFNDEEVNGNQVNFDAATLPLLHHLSVNIVDSTLYNRPAVIALPVDSIN
                     NLPYRPKLKLDYISNSVGVGISTGPSYNTTDMAGSIYMIFSDMVGNHQIYSSLSLNGE
                     IYDFGGQVAYLNQGRKIKWGASLSHIPYRSGNMFWTIDTLKINNTDYIFDNLVLDYIR
                     MFEDNISLFSYYPLSQTRRFEGGISASWYSYRIDRYNNYYDALGYYVGGNRKKLPAPD
                     GISFQTANIAYVEDDSYFGMTSPLQGHRARYQIERYFGNINLYNALIDYRRYLFNKPV
                     GLAFRLYHNGLYGKSSQIELASPMYIGYPWLIRGYEQISVYGGANNVAGYSSLNVNNL
                     VGTRMALVNAEIRIPFSGPKVLAPIKSNIFLTDLNLFLDGGLAWNKGDQIKIKWEPSN
                     FNDRIPVWSTGISMRINLFGYLVIEPYYAIPFQNGGFRNKVFGINFVPGW"
     gene            17121..18074
                     /locus_tag="PLD44_04685"
     CDS             17121..18074
                     /locus_tag="PLD44_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HOF98664.1"
                     /translation="MNNTIARINTCVNNALPKAFKTIIWLLKIILPISLGVSLLQYWG
                     IIATLSVYLTPLFSLVGLPGESAIVFITSIFMALYAPIAIITTLPLEMREITILALMC
                     LISHNLIVETAVQKKTGSSAFIMFSLRIISSFVTAFVLNKILPMNMGHEKIVQAGVSY
                     TDMGSMLLGWLKSSGWLILKISLIITGLMILQNILKEFNILDMLSKIFAPFMKIMGLP
                     KNCSFLWFVAQILGLTYGSAVMIESVENKEISPHDADLLNYHIAVNHSLLEDTLLFAA
                     IGVPVLWMIIPRFVLAVIILWGVHGIRALRRQFSHPEAEIV"
     gene            18131..18733
                     /locus_tag="PLD44_04690"
     CDS             18131..18733
                     /locus_tag="PLD44_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOF98665.1"
                     /translation="MPEKTSRTKKLLIEVARKLFAERGKKNVTMNDIAETSGKGRRTL
                     YTYFKNKDEIYKAVIDSELIKIFQEIQSVSLEKIEPDIKLTKHIHKHLDAIKETVNRN
                     GSLKADFFRDIYEVERTRRKIDIQEIELIKSILREGIEKKLFKRMNVELSSIIILYAL
                     KGLEVPYIRENMGIEFEKNKNSIVDFVLAGIKLPTSKEMI"
     gene            complement(18929..19657)
                     /locus_tag="PLD44_04695"
                     /pseudo
     CDS             complement(18929..19657)
                     /locus_tag="PLD44_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT         6455 a         3563 c         3704 g         6107 t
ORIGIN      
        1 ggtgagccct atcctcccgt caaagttgca tttactaatt gaatttaagt tgtatatatc
       61 ctgtattttt tttgaaaaaa acttgtaggt ttaaaaagag tttataattt tgcttcatga
      121 ttaagctatt aattcctttt ctacaaatcc aagaaaataa ttcaaaaaaa ttcagggaaa
      181 cagttctgat aattcatcaa catttactaa tttcgtgctt gttttttttg acaaactatt
      241 tttttcatgt taaaaaaagt ctcattagct aaggagctaa aaaaatattt tggttttgat
      301 accttcaagg ggaatcagga acaaattatt caaaatgtgc tcaatggaaa cgacactttc
      361 gtccttatgc ctacaggagg aggtaaatca ttgtgttatc agcttccttc tcttttaatg
      421 gatggaacgg ccattgtcat ttctcctctg attgctctga tgaaaaatca agtggatgcc
      481 atgcgcaatt tcagtgaaga agatggcgtg gctcacttta tcaattcttc gttgtcgaaa
      541 caagctattg agcaagtaaa atgtgacatc ctgagcggaa aaactaaatt gctatatgtt
      601 gctcccgaat cactcacaaa ggaagaaaat atcgaatttc tcaaacatat aaaaatttcc
      661 ttttacgcta ttgatgaagc acactgtatt tcggaatggg gacacgattt tcgtcccgaa
      721 tatcgacgta ttcgtcctat tgtcaatgaa ataggaattg ctcccatcat agctcttact
      781 gccactgcca ctccaaaagt gcaacacgat attcaaaaga atctgggaat gttggatgct
      841 aaggttttca aatcttcttt caaccgtccc aatctgtatt atgaagttcg tcaaaaaacg
      901 aacaacatcg acaaggatat tattaaattt gtacgttctc aagaaggaaa atcaggcata
      961 atctattgcc tgagcagaaa aaaagtggaa gaactggccg aaatgttgca tgtaaacgga
     1021 atttcggctt taccctatca tgccggcatg gatgctgctc agcggagcga aaaccaggac
     1081 aagtttttaa tggaagaagc aagcgtcata gtggcaacta tcgcttttgg catgggaatt
     1141 gataaacccg acgtcagata tgtgatacac tacgacatgc ccaaaagtct ggaaggatat
     1201 tatcaggaaa ccggacgcag cggacgtgat ggaggcgaag gtcagtgcat agctttttat
     1261 tcttataagg atctggaaaa actgcgaaaa tttatgcagg gaaaaccact tgccgaacag
     1321 gaaattggca atcaactcct aatagaaaca caggcttatg ccgaatcttc catctgccga
     1381 cgaaaacttt tacttcacta tttcggtgaa gaatacaaag aagataattg tggtaattgt
     1441 gataattgta tgaaacccca aaaatttatg gaagcgcaag aattgttgac tttggttctt
     1501 gaaaccattt ctattttaaa agaaaaattc aaagccgaac acattgtgga cattcttaag
     1561 ggaaatgaaa catcagacgt agtttcctat caacacaatg aattggaaaa cttcggagcc
     1621 gcctccgatg aagatgaaaa attgcttcag gctgttattc gacaagccct tatcagtgga
     1681 tatataacaa aagatgtgga aacttatgga gtgctcaaaa tcactcccaa aggaaaagat
     1741 tatttgaaaa atcctacttc gttcccaatt gttaaagata atgactttga ggaagatgag
     1801 gaagtgaatc tgaaaaatat tgccagcacc agcgctgccg atgaagtatt gttttccatt
     1861 ttaaaggatt tacgcaaaaa actttcaaaa aaattggatg taccgccttt tgtcattttt
     1921 caggatcctt cgctggaagc tatggccact acatacccta taacaatgga tgaactacaa
     1981 aacataccgg gggttggggc aggaaaagcc aagcgatacg gagatgaatt tttaaaagtc
     2041 atcaaagaat atgtgaagga aaacgacata attcgtcctg aagatttgag agtacgtact
     2101 gtagccaata aatcgaaact taaagtctct attattcagg ctattgacag aaaaattgcc
     2161 ctcgacgata ttgccttgtc caaaggattg gatattactg aactgctgga tgaaattgag
     2221 gcaattgtat attcaggtac aaaaattaat atcgattatt ttttggaaga tgttatggac
     2281 gcggaccgcg tagatgaaat tttcgaatac tttaattctg cagaaaccga cagcatagac
     2341 gatgcattgg ataagcttgg aatagacgat tttagtgaaa cggaaatacg gctggtaaga
     2401 atcaagtttc tctccgaagt tggtaactaa accttaaaaa aaggaaagtt tttcagaaac
     2461 aaagtaaaaa gagaaataat cctgtttttt ttcgtatttg ctaattcctc ttgtcaacac
     2521 cccacattaa ctttttcttc aacgtatcga aaaaagtatg ctgaagctgt tttataactt
     2581 ttatggtaaa aggagctttg gcaatgtgca attgtgtagt ttgttccaac actttcgatc
     2641 gcccgtcaag cgctacaaga tagctgtgtg tacgactgtg aacctcaaga tcgatgttcc
     2701 agttgtcggg aattatcaaa ggcctcacat tcaaactatg agaggcaaca ggcgaaagaa
     2761 taaaattttg tgcctgaggt acaacaattg ggcctccaac actcatagaa taagcagttg
     2821 agcctgttgg cgttgaaacc aataaaccat cagcctgata ggtatgaatc aattcgccgt
     2881 tgacgctggc attgatgctg atcatggaag aagaatcctg tttcaaaacc gacacctcgt
     2941 tcaaagcata aggatattgc aacggcgttc cgtccgaagt tttcaccaaa agtaaagtac
     3001 gttcttctat tctgaatttt ccttctaaaa gagcttccaa cgcttcaata atttcatcat
     3061 tcgaaacatc ggtaagaaaa cccaaccgtc cggtattgat gccgataatg ggaatttctt
     3121 tatcaccgat tcttgccgcc gtatttaaaa aagttccatc acctccaata ctcaaagcaa
     3181 catcggcttt gaaattgtca ttctcgattt tttccacgtt ttccaaccga taaccgccta
     3241 tatgtacaag gaaatcgtac aaatcactgt caagcattaa aggaatttgt ttttccgaaa
     3301 aaaaatcgaa cagcacttta atactgttaa tgatgaccgg cctatatata tttccaaaaa
     3361 ttgcaacttt catcagaata taatttattt tttaatggtc tgatggaacc cacatgtctg
     3421 aatattatat tcattcgtgt ttgtatatgt aatacatgtg ggtttcatat aagaattttt
     3481 tttacaaaaa aagaagaaac attctgtaaa acaaggaatt gaattaataa aacgcatagt
     3541 ttttataaaa aaacaaaaac atagacaatt cttcaagtgc gacttcaaaa ttttgagtta
     3601 tttttgtaga gaaaatgaat ctgaaacttc acacaaagga actaaatgaa aacgacattt
     3661 acaaataaat atttttctaa acaaaatgat tttttctctt tttttcatat tgtggaacgg
     3721 agatactatt gtttgtttaa ttttatttaa ccatcaagaa atgaataatt aaaattcaat
     3781 tgacatgaca aaattaagtg taaatataaa taaaatagct accttgcgca atgcacgagg
     3841 tggcaatact cccgacgtct gcaaggccgc catcgattgt gaaaaatttg gtgccgatgg
     3901 cataaccgtt catcctcgac ccgacgaaag acatatccgc tattccgatg tttgggcact
     3961 caaaccaatt ttgcagaccg aattcaacat tgaaggttat ccttctccca actttatgaa
     4021 actggttaca atggtaaaac ctacacaggt tacattggtt cccgatcctc ccgaagccat
     4081 tacttccaat gccggatggg atacggaaaa acatctcgat tttctgaaag acattactgc
     4141 cgaattgcag gatgccggaa ttcgtgtttc cattttcgtt gatgcaaata ttaaaagtgt
     4201 tgaatatgcc gcattggcag gtgctgacag ggtagaactt tataccgaac cttatgcccg
     4261 cgaatacgca gaaaacagag aaaaggctgt tgctccattt attgaagcag ccaaagcagc
     4321 caaaaaactg ggattgggac tgaatgccgg acacgatttg aatcttcaaa atctgcatta
     4381 tttgcatgct tgtattccat ggctcgatga agtttccatt ggtcatgccc tgatttgtga
     4441 tgctttgtat ttgggacttc aggaaacaat aaaacagtac aaaaatcagt tgaaatagta
     4501 aggcatgaat tttgaaacaa aaacaaaaaa ataaaaattg aaataattat gattaatctg
     4561 cttattcttc aagcttcagc aaatgttgcc actacagttg ccgacccgct acagtcggaa
     4621 ataagcctat ggacaatggc gaaatttggt ggtcccatta tgataatttt ggctttaatg
     4681 ctggcttttg ccgtatattt atttatagaa agactgatca cgatcaatca agcttccaaa
     4741 gaagatgctt tttttatgga aagaatcaag gactatattt atgaaggaaa aattgaaact
     4801 gcattaaaac tctgcaggga cacaaattct cccagtgcaa gaatgattga aaaaggaatc
     4861 acccgattgg ggcatccttt gaatgatgtt ctggtagcca tagaaaatgt tggtaatatg
     4921 gaaattgcaa aactcgaaaa gggattagtt attatggctt ctatttcggg tggtgctccc
     4981 atgctgggtt ttttgggtac tgttaccgga atggtatcaa cttttttcaa tctttctcaa
     5041 aaagcaggtg gaaacatctt acttggtgat ctttccgttg gtatgtatca agccatggtt
     5101 accaccattg gcggtttgat tgttggaatt ttggcatatt ttgcctataa ttaccttgta
     5161 tcgagagtag attctgtagt aaacatcctg gaatcgaaaa ctatggaatt tttggatttg
     5221 ctcaatgaac cggtaaaata aatattttaa aacattcgca gcatggcatt aaaaaaaaga
     5281 aataaagttg aagcaggttt cgccatgtcc tccatgacag acattatttt cctgttattg
     5341 cttttttttg ttattgcatc aactttatcg tctcctaacg acataaaatt aaatttgcct
     5401 gaaagtagat ctaagtcttc aaccaagcaa ataattgcta aagttgctat tgatgctgaa
     5461 ggaaagttat cggtagccaa aggaaatcaa aaacccgttc ctattgaacc taaattgttg
     5521 gaatcgtatc ttttaaatat ggcctcacaa gacagtattt cttttgttgc actttatgcc
     5581 gatcaaaata ttgcttacaa ggaaatagta agggttttgg atatcgccaa tcaacatggt
     5641 tttaaaattg ttatagccac caaaccgttt gaaaattgag aaggtgtgaa aaaatccgaa
     5701 ttatatggta ttgttgggtc aatcatcttt tgtatgatgc tgctggtgct attgctgatc
     5761 attaaaatgc acgcttcaac atctgttgaa cctgaaggca ttatggtcag ttttggtgaa
     5821 actttggaag gaggcagcag ggcagcttcc ccttctgctg cagaggagta tgcagtatcg
     5881 aaaaaagcaa gtgaaaaaat tttgactcaa aaagatcctt ctgtttcgct cgacaaaaag
     5941 actgacaaca gagagcagga aagagaattg tccgagaaaa aaagacttga acaagaggca
     6001 atatcaaaag ccaatattat ggaagggata tttgataaaa agatatcagg gaatgaaaat
     6061 gtgggcgatg agaatttatt aaaaggaaat cctgccggaa gaggaacttc aaatgggaac
     6121 agctggtcgc ttagtggacg aaatttggtg ggaaatctga ttacccccag atatgaacaa
     6181 aatgtggaag gtgaggttac tgtcaacatt cgggttgacg aaaacggagt tgttgaggga
     6241 gtttctattg gtgttcccac caatatttcc gatgcaacaa ttttaaaagc agccatggaa
     6301 gcagcaaaaa atacccgttt cagtcccgga aaggggaaag ctaccggaac catcacctat
     6361 catttcagat taaaataaac taccggaatg tggctgtttt acagaaatat gtggaaaaat
     6421 aaaaaacgac cttaacataa taaaaatgaa aaacttatga aagcaatggt atttttggct
     6481 gatggttttg aagaaatcga agccatcgct ccaattgaca ttatgcgtcg agcaggaata
     6541 gaagttacaa ccatttccgt ttcacgcgaa aaagaagtga gaggtgcaca tggtgttttg
     6601 gttgaggcag atcgtttgtt tgaagaagtc agtttttctg atgccgattt gctgtttctg
     6661 ccgggcggaa tgccggggac aaaaaattta aatgctcatg acggtttaaa acaacttttg
     6721 ctgaaacatg ccaatgaagg caaaaagttg gcggctattt gcgctgcacc ttccatattg
     6781 ggcgaattgg gattgctcaa gggaaaggaa gccgtttgtt ttcccggatt tgaagaaaca
     6841 ctaaagggtg ccattgtatc cgataaaaaa gttgttcagt cagggaatat tattaccggt
     6901 aaagctgccg gcgccgccat agaatttgcc ctgaaactgg ttgaaaatct gaaaggaaaa
     6961 tctgcagccg agcaggtagg tttgtctatt tttgcttttt aaagagaagc taaaaacaaa
     7021 aaagcaaaag agttatcaaa aaaacaaagg aggaattaaa aaaataattc ctcctttatt
     7081 aaaactttaa caaacaaatt aattgatagc atcgcttaat tctgcaccgg ctttgaattt
     7141 tgcaaccttt ttagcaggga tagtgatggg ttttttggtt gccggattaa taccggaacg
     7201 agaagcacgt tcagcaaccg agaaagtgcc aaaacctact aaactaactt tatctcctgc
     7261 aattaaagct tcagagatac ttttaacaac agcgtcaatt gctttctttg catcagcttt
     7321 gcttagccca gatttttctg ctaccgcact gattaattct gctttgttca taacataaaa
     7381 tttttttaaa gttaaacaca ctaaaaaatc ctttttaaag ctacaaatgt acattattga
     7441 aaacaaatag caagcatttg aataaaaaaa tttctgtatt ttttactttt tcttacaaaa
     7501 tatgttacaa aacatgtttt agttaatttt ttcgctacat ttgcaattta aaaaaacgat
     7561 ttaatgaact atatactcca ttaaatgttt aaaagttggt aaatggttgt tgaaagaaga
     7621 ttttgtctga acaattttct tgaataaaaa aataagtatt gcatgagtta cagatcctct
     7681 ttttttggtt ctatacctgt ggtggttaag aatctgattt taataaacat catattgtgg
     7741 ttggcaactt tggttttacc tcgttttttt ctgcgttggg ggataaatgt tgatctggtc
     7801 aacattctcg gtatgcatta ctggggatcg aaacagttta atcctgctca actgatcact
     7861 tacatgtttt tgcacggaaa ttttgagcat atttttttca atatgtttgc cttgtttatg
     7921 tttggaacgg tattggaaca attttggggt cccaaacgct ttatgctgta ttacatggtt
     7981 acaggtattg gagctgctct tatacagcaa cttttttggt ctgtagaata tcagccggta
     8041 atttcagcac tcaacgaagc cataaacacc gggaacaaag atgccttgtt ttctcacgaa
     8101 ggagtattgt cccgtttttt tagaatcaac aatttggcag ctttagacac cacttcatta
     8161 attgaactga agcatctttt tgtgaatcta cccgtaacca taggtgcttc aggtgcaatt
     8221 tttggtttat tactggcatt tggatggctt ttccccgatg ctaaacttat gcttatattt
     8281 ctcccgattc ctattagagc ccgaatattt gtattgatgt atgcagtagc cgaattattt
     8341 ttaggggtgg ctggttttac gggtgataat gttgctcatt ttgcccattt gggcggtatg
     8401 ctcttcggaa tttttttgat tttgtattgg aagaaaaaag gtgtacattg atgtaagtat
     8461 tcttaaaaaa tttatataaa gaatataatt tattgattta cattgtttta ttttcaaatt
     8521 ttttgctatg aatatttttg acaatattaa attatcctat cgtcagggaa gtcatctgat
     8581 taaactcata tatatcaacc tcggtgtgtt tttgctgtac catgctgttt taatcacttt
     8641 caaactattc aatgccagtg gtatttttct tcttccattt ttggctatgc cggcagatgg
     8701 aaggcaattg ctttatcatt tctggacacc ggttacttac atgttcatgc acgaaggttt
     8761 ccttcatctg tttttcaaca tgttgtgcct ttactggttt ggaaaaatat ttttgatgta
     8821 ttattccgaa aaacaactta ccggtttata tctggtggga ggatgggtag ccggattgtt
     8881 ttattttctg tcatttaata ttttccccta tttcgatgca gctgtttcac aaggtttact
     8941 tttgggtgct tccggttcta taatggccat tattgtagct tcagccgtcg cttcaccgaa
     9001 tctggaaatg cggatactgt ttctcggcgg cataaaattg aaatacattg cattggtggc
     9061 gatccttaca agttttttcg gaatcacgtc caacaatgcc ggaggtgaaa ttgctcattt
     9121 gggaggagct ttaatgggtt acctttttgt ggtatcttta cgtaaaggaa aggatattac
     9181 caaatggcta aatggagtgc tggataacat tgtgaatctt ttttcgagaa aaaaactcaa
     9241 aataaaaaaa cccaaatatt caacttctta ccgaaaaatg accgatgagg agttcaacat
     9301 ggaaaagtcc cgacgaatgc aagaaataga cagaatactc gacaaaatta aaacatcggg
     9361 ttacgacagc ttgacggcag aagaaaaaaa aagacttttt gaacagggaa aaccaagata
     9421 aattttttgt cgatccaatc ggaaatcgtt tctttcccca aaatcaatta ttgaaacaga
     9481 cggtaaaatg atcctaaaga aaattacaaa atggggttta attagcgcaa atattattgt
     9541 agcatgcgct ttgcttttca gcgtttttgc aggttttgta agtcctgcgg tatttacttt
     9601 ttttgcttat tttgcacttg tttttcccat tatttttttt cttaattttg catttttcct
     9661 tttatggatt gtgctaaaga aatggtattt tctcatttcc ctgagcttaa tgcttgtttc
     9721 tttccctcaa ttaaaaacaa atttttcttt tcatttaaaa aaacaaccgg tttcttcaat
     9781 tcagccagac tttaccatta ttagttacaa cactcacatg atgggactcg aaaaaaaaca
     9841 cgatgaagca aacaaaaaaa taaatccggt tattcaatat gtactttcaa ccgatgccga
     9901 cattgtatgc ttacaggaat ttatggtcag cacaaacgaa aaatatctta ccgaagaaga
     9961 tgccatgaaa gcttttagca aatatccgta tcatcatatc tatttcaaag cacatgaaaa
    10021 aaaaagcttg ttgggaattg ccacattctc taaatttccc attatcaatc gacaaaccat
    10081 accctacaat tcttcagcca atgcttccat ttatagtgat ataaacattc acggaaaaac
    10141 cataagggta atcaacaatc atcttgaatc caatcagttg acggaaaagg acaaagccat
    10201 gccctatcag ttgaaggata atttcaacac cgatttggca acgaacataa ctgtttattt
    10261 ttcaaaaaag cttggagccg cctacaaact cagagcaatt caggccgatg aagttaaaaa
    10321 ggttatttct tcttctcccc atcctgttgt tgtttgtggc gatttcaatg atgtcccttc
    10381 ttcgtatgta tatacaaaaa ttcgcggaaa actcaaagac tgtttcttgg agcagggaaa
    10441 aggatttgga tggactttca acgaaaagca ctatcgattt cgaatcgatt atattttcca
    10501 tgacccgtct ctggttttat tgaatttcaa aaaagataaa gttccctatt ccgatcattt
    10561 ccctttgaaa gcagcttttc aaaaattata gaatgttggt cttttaccca agaattttgt
    10621 cagtagaaaa gacaatggat tctttaagaa taaaaagcta ataaaaattt tttcatagct
    10681 taaattattt gtttttaggc atatagaatt tatcccaata agaaatatta aaaaaacatt
    10741 gaaaattaaa tgaaagaaag cgtataaatt cttattttag tgctctgaaa taaaacaaaa
    10801 tatcaatatg ttgaccatag gaattgccgg aggaacaggt tcgggaaaaa ccaccgtcgt
    10861 tcgcaaaatt atagaaagat tacctgccgg agaagtagtg gttttaccac aagattcgta
    10921 ttatcgcgac agcagttttc ttcctctcga aaaaaggttg gagattaatt ttgatcatcc
    10981 cgattccatt gaatttgaac tgatgataaa acatttgcaa gacctcaaag aaggaaaacc
    11041 catcgaacag cccatttatt cttatctcac gtgcacgcga gcagccgaaa ccgttacaat
    11101 ttatccacgc gaagtaatca ttgtggaagg aattttggtg ctgtgcaatc cggaattacg
    11161 tgaactaatg gatctgaagg tttttgtgga tgccgatccc gatgaccgtt tgattcgggt
    11221 tatcaaccgc gatattgaag aacgcggtcg tacggtgaac aaggttatcg aacgttacga
    11281 gcataccgtt aaacccatgc atgaactttt tattgaaccc accaaacgtt ttgccgacct
    11341 cattattcca cagggtggag ataatcatat tgctattgat attcttacca aatatatcga
    11401 aaacaacctg aaataagaaa aaaaaccgca aaacaatatg acaaacgccg atttgcaaaa
    11461 aataatgttt tttgatattg aaactgttcc tcaaacttca agtttttctg agctttctaa
    11521 tgaaatggcc caactttggg aagagaaatt tcaaaaactt agaatcagga tgcccgaaaa
    11581 atacaaggag gacacatcgg caagcgaagc tttcgatact tcagccggaa tctattccga
    11641 atttggcaaa attgtctgta tttccgtagg ttttatctat tttcagggag aaaccatgta
    11701 ttttagaacc aaatcgtttg cttccgacga tgaaaaggaa cttttaaaaa atttttccga
    11761 cctgatttcc aaattttgct catcaaaaga caatatgctt tgcggacaca acatcaagga
    11821 atttgacata ccctatattt gcagacgcct gcttatcaat ggattggaac ttcctcccgt
    11881 atttcaaata tcggggagaa agccgtggga aaacaatttt ctcgataccc tggagctttg
    11941 gaaattcggc gattacaaaa attacacacc cttgaaattg ttaaccgctg ttttcggaat
    12001 tccaactcca aaagatgata ttgatggaag tcaagtcgct caggtttatt acaaggaaaa
    12061 agatcttgaa cgcattgcca attattgtac caaagacgtc attgctaccg cacaggtatt
    12121 tttgcgctta aacggatttg aattgatttc accccaaaat atagaaaatc tataacattt
    12181 cgataatgca acgaaatata atattcatcg tttatctcct gattattata ttcttaacat
    12241 cctgtcgaga aggggaaaag gtaaaattat cttccgaaaa ttctttcaga gatattcaag
    12301 aaattgtcaa atccgataca ttaagagccg gaaccatgta tggctcaaca tcctatttcc
    12361 ttttcagaga agaatggatg ggttatgact acgaaatggt gaaaaatttt gccgactacc
    12421 tgaaagttaa tttaaaggtg attccggcag ctaccgagtc cgaactattt caactgctta
    12481 aagaaggaaa aatagatatt gctgcatata accttcctgt aaccaaagct ttaaaaaagc
    12541 actttctcta cgtttttcct caacctcaga cgtatatggt attggttcaa aatatggggc
    12601 ggaaagcgct ttcaagtgta acggatttga taggaaaaga agtttatgta aaaaagaata
    12661 cactttttta tcaaagactg aatgccttaa acaaagaaat tggcggtgga ataaaaatag
    12721 ttatcgctcc cgattcggtt agtaatgatg aattgataga aatggtggcc aacaataaaa
    12781 ttgattatac cattgcttac tatgacaaag ctttgttgca caaatcatac tataagcgtc
    12841 tcgattgccg tttacccgtg agttttgagc aacccaacgg ttggttaatt agaaaagaaa
    12901 acactgattt gttcaaaacc ctttcaacat ggggtcaact ggctggaaca caaagaaccg
    12961 aagatcgact tatcaactta tactgggaaa aaagtccgta ttttattacc cgtaaaataa
    13021 aaatacccaa agggtccatt tctccctatg acgaaatgtt taaaaaatat gccccaacta
    13081 taggatggga ttggcgaatg ctagcagcaa tagcttttca tgaatccaag tttgattcgg
    13141 cagaaatatc aagggcagga gcggtagggt tgatgcagct catgcctcaa acagcagcct
    13201 cttttggtct taatcatgaa aactggttca atcccgaagc aaatttggat gcagcagtac
    13261 aatatattaa aagtttaaat ttgattttca gagaaataaa agataaagat gaacgaataa
    13321 aatttatttt ggccagctac aattcaggac cttcgcatgt tctcgacgca atggctctgg
    13381 ccaaaaaata tggtaaaaat ccccatatct ggttcaacca tgttgaaact tatctcctaa
    13441 aaaagactga tcccgattac tatcgcgatc cggttgtaaa atatggcttt tttcgcggtg
    13501 cggaaaccgt tcggtatgta caaaatgtat tggacactta tgagaaatac ttaagcagaa
    13561 aataagacat tttttcatag ttgttccttt ttattccatt gttttacaag aattttctac
    13621 aatttttcat tctggcattt attttgttta aaactttttg ttctttttac ccgagacaaa
    13681 agcgacatga tttcagattt gttttattga ttgtttgttt ttcatagtca attgaattga
    13741 gaaccacaga aagttgaatt atgaaaaaat taattgttaa accgctcata attgcgttcc
    13801 agataatttt aatactttta ccgactgacg caaaagccca gtattttgga cgaaataaac
    13861 caagctatca gacttttaag tatcaaataa agcagactcc gcattttgaa atctatcatt
    13921 accttactaa cgatacggta atcaaatatt tcgcaggttg gactgaagaa tggtatcggt
    13981 ttcatcaatt gatattcaaa gatactttta aaaccagaaa tcctctgatt ttgtatgcca
    14041 atcatgccga ttttcaacaa acaaatgcta tcagcagcat tatcagcgaa ggaacgggcg
    14101 gcgtaaccga aagtctgaaa aatcgcgtta ttctgccttt tgctcctacc ttataccaaa
    14161 ccgaccatgt gctgggacac gaattggttc acgcttttca ataccacaaa ctgttaaagc
    14221 cagattcggc caagtattac aacataaaca atcttcccct gtggatggta gaaggaatgg
    14281 cagaatatct ttccattggg agcgttgatg ccaacacttc catgtggatg agagatgcac
    14341 tgattcagaa aaaatttcca tccattaacg atttgaacaa tacttccgaa tattttcctt
    14401 atcgctatgg acaggcttta tgggcattca taggtaaaac atggggcgat tcgataattc
    14461 tgccgttatt tgaaaaaacc gcccaatacg gactggatag agcgattgac agccttttcc
    14521 actttaacga aaaaaccctt tccggcatgt ggaaaacagc tacggagaat cattacggaa
    14581 aattaatgaa agatacgacg tataatgtta tcgggaagca aataatttcc aaaaaaaatt
    14641 caggttctac caatgtttcg ccatctttaa gtccggatgg taaatatatc gcattcttct
    14701 ccgaaaaaaa tgtttttacg cttgatttat atttggctga tgcgcaaaat ggaaaaataa
    14761 tcaagaaatt atggagtgtc aaccgaaatc aggaaataga tgatttcagt tttatagaat
    14821 cgggaggaac ttggtcgccc gacagcaaac aatttgcttt tgttgtttac agcaaaggcc
    14881 gtcaaaaact ggctattgta gatgtacgca aagccaaaat gaaacttttg gaaataaaag
    14941 gattaaattc attttcaaat cccgaatggt cgcctgacgg cagatatatt gtcgttacgg
    15001 gtttggtaaa cgggattggg gatttatatc tttacgaagt aaaatcagga aaaaccgaaa
    15061 aactgactca cgatttgttt tccaatattc atgcttcatg gtcttcggat ggcaaaaaaa
    15121 tagtatattc ttccgaatac ttatgtggtg ccggaagtaa aaaatttgca tttcatataa
    15181 acattctcga cttggaaacc cgagaaataa aaaccttaga tgttttccca aaagccgata
    15241 acctgaatcc tcgcttttca aacaataacc aatatattta ttttgtttcc gatgctgatg
    15301 gattcagaaa tctgtataaa tatgatttga acaccgataa agtttttcgt ttaacgcaat
    15361 acatgacagg aattagcgga ataactacat tctcgccagc aatcagtaca gacagaaaaa
    15421 atgacttgat tgcctatact tactatttca acggcaatta cgaaatttat acagcttccg
    15481 caaccgaatt taacgacgaa gaagtaaacg gaaatcaagt aaatttcgat gccgcaactt
    15541 tgcctttgtt gcatcattta tccgtcaata ttgttgattc tacgctttac aatcgcccgg
    15601 cagtgattgc acttcctgtc gattcaatca ataatctgcc ctatcgaccc aaattaaaac
    15661 tcgattatat ttcaaattcg gtcggggttg gaatttcaac aggtccaagt tacaacacca
    15721 ccgatatggc cggtagcata tacatgattt tcagtgatat ggttggcaac catcaaattt
    15781 attcttcctt gtcgctgaat ggagaaattt atgatttcgg cggtcaggta gcttatctga
    15841 atcagggaag aaaaatcaaa tggggagcat ccttatcgca tattccctac agaagcggca
    15901 acatgttttg gacaattgat acgctgaaga taaataatac cgactatatt ttcgacaatc
    15961 tcgttttgga ttacatccgc atgtttgaag acaatatttc gctgttcagt tattatccac
    16021 tctctcaaac acgtagattt gaaggaggaa tttctgcatc gtggtactca taccgcatcg
    16081 acagatacaa caattattat gatgcgttgg gctactatgt gggaggaaat cgtaaaaagc
    16141 ttcctgctcc ggatggcata agttttcaaa cagcaaatat tgcttatgtg gaagatgatt
    16201 cctacttcgg catgacctcg cccttgcagg gacatagagc aagataccaa atagaaagat
    16261 attttggaaa catcaattta tacaatgctt tgattgatta cagaagatat ttgtttaaca
    16321 aacctgttgg gctcgcattt cgactttatc acaacggttt gtacggtaaa tcttctcaga
    16381 ttgaactggc ttcaccaatg tatattggat acccgtggtt aatcagagga tacgaacaaa
    16441 tatcggttta tggaggagca aacaatgttg caggctattc ttctttgaat gtcaataacc
    16501 ttgtgggaac acgcatggca cttgtcaacg ctgaaattcg tattcctttc agtggaccga
    16561 aagtactggc tcccattaaa tcaaatattt ttcttaccga tctgaatctg tttttggatg
    16621 gtggattggc atggaacaaa ggagatcaaa taaaaatcaa atgggaacca tccaatttca
    16681 atgatcgtat tcctgtgtgg agtacaggaa tttccatgag aatcaatttg tttggctatt
    16741 tggtaataga accttactac gccataccat tccaaaatgg aggtttcaga aataaagttt
    16801 tcggaatcaa tttcgtaccc ggatggtaat tttaaaacta atttaatttc ttgaatatct
    16861 ttttgttaat acagaatcaa aacgaaaagt ctcatatttt cagtagtcga aaaagcaaga
    16921 cgatgcatca ataagttaaa tgttgttaaa tatcaatttt tttctccgaa tatgttgtac
    16981 aacataaaaa atcgatgtac atttgcacat gtttttcatg gtattagatt taaggttaat
    17041 taaaaagatt gggttgtcgt gagacaacct ttcttgtttt tataacttcc attctttgca
    17101 atttttttta ctttacttcg atgaataaca ccattgcccg aataaataca tgcgtaaata
    17161 atgcactccc aaaagctttc aaaaccatta tatggctttt aaaaattatc ttaccaatca
    17221 gtttgggagt cagtttactg cagtattggg gaattattgc caccttatcc gtttacctta
    17281 ctccattgtt ttcacttgta gggcttcccg gtgaatcagc catagttttc attaccagta
    17341 tttttatggc attatacgct cccatagcca ttatcacaac attgccactg gaaatgcgtg
    17401 aaatcaccat cttggctctc atgtgtctga tttctcataa cttgattgta gaaactgccg
    17461 ttcaaaagaa aaccggttct tctgcattta tcatgttctc gctgcgtatt atttcttctt
    17521 ttgttacagc ttttgttctg aacaaaatac ttcccatgaa tatgggacac gaaaaaattg
    17581 tacaagcagg cgtaagctat accgatatgg gaagtatgct tttggggtgg ctgaaaagtt
    17641 ccggttggct gatcttaaag atttctctca ttattaccgg attgatgatt cttcaaaaca
    17701 ttttgaaaga attcaacata cttgacatgc tgtcgaaaat atttgctcca ttcatgaaaa
    17761 taatgggatt accgaaaaac tgttcttttt tatggtttgt agcccaaatt ttaggattaa
    17821 cttatggttc ggctgtcatg atagaaagtg tcgagaacaa agaaatcagc cctcatgatg
    17881 ccgatttgtt gaattatcat attgcagtaa accattcctt gctggaagat accttacttt
    17941 ttgctgctat tggagttcct gtattgtgga tgataattcc tcgtttcgtt ttggcagtta
    18001 tcattttatg gggtgtgcat ggcatcagag cattgcgccg ccaattttct catcctgaag
    18061 cagaaattgt ttgacaatca ataaaaaaac cgagctgaga aaaattttcc ctgtttcaaa
    18121 catatttttt atgcccgaaa aaacatccag aacaaaaaaa ttattgatag aagttgcacg
    18181 aaaattattc gctgaaagag ggaaaaaaaa tgttaccatg aatgacattg ccgaaacttc
    18241 agggaaagga agaagaactt tatatactta tttcaaaaac aaagatgaaa tatataaagc
    18301 cgtaattgac agcgaactga tcaaaatttt tcaggaaatt caatctgttt cattagaaaa
    18361 aatcgaaccg gacattaaac ttaccaaaca catacacaaa catttagatg ccataaaaga
    18421 aaccgtaaat cgaaatggtt cgctcaaagc cgattttttt cgcgatattt atgaagtaga
    18481 acgcacccgg cgtaaaattg acattcaaga aattgaactc attaaatcca tcctgagaga
    18541 aggaattgaa aaaaaactct ttaaacgtat gaacgtcgaa ttgtcctcta tcattatctt
    18601 atatgctttg aaagggctgg aggttcccta catcagagag aatatgggta tcgaattcga
    18661 aaaaaacaaa aacagcatcg ttgactttgt actggccgga attaaactac cgacttcaaa
    18721 ggagatgatt taatttggat taaataagaa agtacgctat taagatacca tacttaccca
    18781 caaaatagaa cactcctccg ctgataacaa aagattatat ggaaaacact gttttttgcc
    18841 ggatgctttg tggtaaaaaa tcactactgc ctgatgattt ttagtgttag ttttttattc
    18901 attaaatttg aaagttgtat tcatttcaaa atgttcccaa ccacgacttc aattctgtca
    18961 acatttccaa atggtatttg aaagattctc tgattcccca gccatgaccg ccgtcggaat
    19021 aaatatgcat ggttgcagga atgttttgct gttgcaatgc caaatagtat tttacagaat
    19081 tcacaggttt taccacatcg tcgtcatccg acaatacgat aaaagctcgc ggtgtgttgg
    19141 cattgacctg caattcgtta ctgtataatt tttccatttc aaatgttgga ttttttccta
    19201 gcagatgttc gcgcgaaccg gcatgtgttt cttgtttgtt catggtgata accggataaa
    19261 acaaaatttg aaaatcaggc cgtaattctg taggcgcatg agttgccact gtagcagcca
    19321 aatgacctcc ggcagaagaa cccattatcc caatacaatc gggatgaata ttccaagttt
    19381 tggcattcgt tttagttagt cggatggctt cataagcatc ggaaattggc acttccaaat
    19441 gtccgttagg catgcggtat tttaaaacaa taaccgctat tccctgattg ttgaaaaacg
    19501 aagcccaatc gtaaccttca tgattcatgg ctaaatgctg ataaccacct ccggggcaaa
    19561 taactacagc ctttgttggt tgcgaagctt tgggaagata aacggtaatg cagggtttga
    19621 agttcgattt ggcatcatcg taacccaatg tttccattcc gttggtattt ggtaatccgt
    19681 tttgccagag atcaatagta aaaggtgatg ttggagtgga aatttcttta tcctgtgcac
    19741 caacaaaagg acaaaccacc aacaaaagca gcaaaaataa gggctgaaaa gaataaaatg
    19801 ttttcatcag aatataattt attttttaa
//
LOCUS       DAOSIB010000035        19822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_2986_length_19822_cov_13.586078, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000035 DAOSIB010000000
VERSION     DAOSIB010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19822
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2986_length_19822_cov_13.586078"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PLD44_04700"
     CDS             complement(<1..319)
                     /locus_tag="PLD44_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98666.1"
                     /translation="MKNFFYSFGSWLLAFSLAMFLFNFSFILQVSAQGAGKALNFNGA
                     HKYVSVPNSSTLNVSNAMTLKAWMKSAEENFSYRVPITLNPATPIADYQVKVELTTSN
                     FAYA"
     gene            1161..3410
                     /locus_tag="PLD44_04705"
     CDS             1161..3410
                     /locus_tag="PLD44_04705"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOF98667.1"
                     /translation="MIFDEAMIQQFYESFPEKIEQIKNRLGRPLTLTEKILYTHLSAD
                     ETLKNFVRGIDYVNFSPDRVAMQDATAQMALLQLMNSGRSKTAVPATVHCDHLIQAHV
                     SAEKDLDTAIKTNSEVYNFLRAVSNNFGIGFWEPGSGIIHQVILENYAFPGGLMIGTD
                     SHTPNAGGLTMLAVGVGGADAVDVMSGLPWELKMPKIIGVKLTGKLSGWTSPKDVILK
                     LTGMLTVKGATNAVIEYFGEGTASLSATGKATICNMGAEVGATSSIFPFDNKSAVYLE
                     ATGRNSIAETAKKLAEHLQADSEVVQHPEKYYDRLIEIDLSTLEPHINGPFTPDAAYP
                     ISIFGKVVREKNYPQKLEVGLIGSCTNSSYEDLSRAVSVLRQAREKNLKVRSQMIINP
                     GSEKIRSTAERDGMLDEFKSSGAIIMANACGPCIGQWKRDIDVADRPNSIITSFNRNF
                     AKRNDGNPNTHTFLSSPEIVAALSIAGDLCFNPLTDELENEKGEKIKLDEPQGFELPL
                     LGYEIKESGYIAPSEEKMEINIDPKSERLQLLQPFPAWDGKDMIELPLLIKVKGKCTT
                     DHISMAGPWLRFRGHLDNISNNLLMGAVNAFNDKTNAVLDPETKQYMAVSALARKYKA
                     KGTGSVVVAEENYGEGSSREHAAMEPRYLNVKAIIAKSFARIHETNLKKQGMLALTFI
                     DKSDYDKIREDDRISILGLSDFKPGKNLLVQLHHSDGSVEQFKVAHSFNETQIEWFKA
                     GSALNAMRK"
     gene            3501..4856
                     /locus_tag="PLD44_04710"
     CDS             3501..4856
                     /locus_tag="PLD44_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOF98668.1"
                     /translation="MKEELLIYKLSETIKKTSQIDKELFTKYNVKRGLRNEDHTGVLV
                     GLTRIGDVVGYERTEDGQLKAIPGKLYYRGIDVEELVHGIQSENRLGFEETAFLLLSG
                     YLPTKDELGTFKKILNEAMPLQHQATMNIMALKGHDIMNILARCVLEQYIYDEDPDNI
                     SRDNLIRQSINLVSKFPTIIAYAYNVLRHHEKGRSLHVRHPDENFSFAENFLFMIKGK
                     GQYTPLEIKILDLALILHAEHGGGNNSTFSVRVTSSTETDTYSSIAAGIGSLKGPLHG
                     GANLKVMGMIKEMRENINDWTDADEIDQYLKKLLNKEAYDKTGLIYGIGHAVYTISDP
                     RAILLKDMARELAKEKGLEKEFAFQELIEQRAVEVFMNYKNGNNNKQVCVNVDFYSGF
                     VYEAIGIPKEVYTPIFAMARIVGWCAHRIEELNFTSKRIIRPAYKNVNEIQQFIPLEK
                     R"
     gene            4919..6145
                     /locus_tag="PLD44_04715"
     CDS             4919..6145
                     /locus_tag="PLD44_04715"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HOF98669.1"
                     /translation="MKKIKVEQPVVELDGDEMTRIIWRIIKEQLILPYLDIDIKYYDL
                     SIQNRDRTNDEVTIEAAHAIKKYNVGIKCATITPDEARVKEFGLKKMWKSPNGTIRNI
                     IGGTVFREPIICSNIPRLVPNWTKPIVIGRHAFGDQYKATDVLIKGKGTLKMTFTAEN
                     GETQEWEVYNFQGDGVAMSMYNTDESIYGFARSSFQAALDKKWPLYLSTKNTILKTYD
                     GRFKDIFQEVYEKEFREAFQNAGITYEHRLIDDMVASVLKWNGGFVWACKNYDGDVQS
                     DSIAQGFGSLGLMSSVLITPDGKTIEAEAAHGTVTRHYRLYQQGKETSTNPIASIFAW
                     TRGLAHRGKLDGNNALIDFARKLEEVCVQTVESGKMTKDLAILVHGEKVSRTDYLSTE
                     EFLTALKQNLEAKLSA"
     gene            complement(6237..8522)
                     /gene="rpsA"
                     /locus_tag="PLD44_04720"
     CDS             complement(6237..8522)
                     /gene="rpsA"
                     /locus_tag="PLD44_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOF98670.1"
                     /translation="MEENQINLEKNESALAENVSPEAVEAKKEETQEVKEVLSPEQES
                     VAESHAAEPIVAETPEVSVPEEPVKESVAPVQAEITPEEFDWDAYEKGNTLTTEAKKE
                     LENVYDKTLNAVKDKEVVEGTVISINKREVVVNIGYKSDGVVPMSEFRYNPDLKVGDK
                     VEVYIESQEDKKGQLLLSHKQARISRAWDRVNAALESQEVVKGFIKCRTKGGMIVDVF
                     GIEAFLPGSQIDVKPIRDYDAFVNKTMEFKVVKINQEFRNVVVSHKALIEEELEQQKK
                     EIISKLQKGQILEGVVKNITSYGVFIDLGGVDGLIHITDLSWGRINHPSEVVSLDEKI
                     NVVILDFDEEKKRIALGLKQLTPHPWDALDPNLKVGDKVKGKVVVITDYGAFVEIAPG
                     VEGLIHVSEMSWSQHLRSAQDFLKVGDEVEAIILTLDRDERKMSLGIKQLKSDPWENI
                     EERYPVGSKHVAKVRNFTNFGVFVEIEEGVDGLIHISDLSWTKKIKHPSEFTQIGSEI
                     EVVVLDIDKENRRLSLGHKQLEENPWDALESVFAVDSIHDGVVVEMLDKGAVIALPYG
                     VEGFASTKQLVKEDGTQAQVDEKLPFKVIEFNKDAKKIVLSHTRIHEDLKRAAKTESA
                     EGETSTSPKKSVTKRTKKEESSTVQTQAIEKTTFGDIQSLVDLKDQMESEAAKKLAKK
                     ETKSKKAEPAEKEEPAEVKVAEETKVEEPSEKPAETEEKSEKPQKSSKKEKTEKVEEK
                     VVEAEETPASEEAENSEKPIE"
     gene            complement(8640..9359)
                     /locus_tag="PLD44_04725"
     CDS             complement(8640..9359)
                     /locus_tag="PLD44_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOF98671.1"
                     /translation="MKSKYFVNILIMTMLCFYMQQLNAQDWPNLGRFRAENEKLGAPD
                     KGEKRVVFLGNSITEGWINIRPDFFKNRSYINRGISGQTTPQMLLRFRQDVIKLQPAV
                     VVILAGTNDIAGNTGPSSLEMIEDNLSSMSELARVHNIKVVLSSVLPAYDYPWRRGLQ
                     PAEKIVTLNAWIKDYCQKNGFVYLDYFSAMADERNGLKSEFSEDGVHPNQKGYQVMEP
                     LAEAAIQKALKQNFKKCPCNK"
     gene            9644..10921
                     /locus_tag="PLD44_04730"
     CDS             9644..10921
                     /locus_tag="PLD44_04730"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HOF98672.1"
                     /translation="MKTHTKKFYFPNFEKIICLFLLLTGFFSKTLSQDFAKGADVSWV
                     TQMEAAGKKFYTAEGVETECMTLLKSLGMNTIRLRVWVNPADGWCNAQDVLLKAQRAN
                     DLGMRIMIDFHYSDSWADPGKQTKPAAWSTLSFADLKTALSQHTNEVLTLLKNNDITP
                     EWVQVGNETSDGMLWEDGRISKNPQNYAELNNAGYDAVKSVFPNAKVIVHLPNGYSNS
                     LYRWFFDTLKNYGGKWDVIGMSVYPFWYTTKNDWRNCNISCFNNMNDMVSRYGTEVMI
                     VECGMQWDAPEACRDFLSDLINKTKNVSQGKGLGVLYWEPQCYPGWNGYTLGAFDNTG
                     KPTIALDAFKEVSQSTHPLQLKTGWRYDDLQKVILFDKTFSKVTLFDSAGTAIQSFAS
                     TNRISMKNMQKGIYILHAALKNTEPLLIKFIYN"
     gene            11027..13492
                     /gene="priA"
                     /locus_tag="PLD44_04735"
     CDS             11027..13492
                     /gene="priA"
                     /locus_tag="PLD44_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOF98673.1"
                     /translation="MKYIEVILPLPLPNTFTYAVPEADENSIQQGMRVVVPFGKKKLY
                     SGIVYYVHILRPETTYQIKEIVGILDEEPILRRPQIKFWEWIANYYQAHLGEVYQSAL
                     PAGLKLESETLVRVKPDFDTNVHFSEREKKILDVLLNQKKPISLSALAKDSGISNVLP
                     QLKLLLEKDIIEISEQLTEKFHPKTETYIRLRPEIQQDEKLHEIFDQLKSAKKQLSLL
                     MNYIDLSKCLDPKNVREVSRKILLEKSGISPAVLKALTDKKIFEIYEKIVGRLDFSED
                     ELQKPSILNEFQTKALQEIEKKFKEKPVVLLHGVTSSGKTELYIHLIQKVLNEGKQAL
                     YLVPEIALTTQLTARLKRIFGKKLGVYHSKFSDAERTEIWNNLLNDKGYEVIIGVRSS
                     IFLPFRQLGLVIVDEEYETSYKQFDPAPRYHARNAAIVLASMHGAHTLLGSATPAIES
                     YFNAVTGKYGLVKLDRRFEEISLPEIVVVDLKEAYHKKQMNGHFSDLLMHEMQKTLQN
                     REQIILFQNRRGYAPYLECKSCNYIPKCKNCDVSLTYHKTTNALTCHYCGYTESYPAS
                     CPVCGTPAAFSTHGFGTEKIEDEIKKIFPEARISRMDLDTTRSRKSYETIITDFEEYK
                     IDILIGTQMITKGLDFNKVSLVGILNADNILNFPDFRSHERAFQLLSQVSGRAGRKDK
                     QGKVILQTYQPKHPIIQQVIANDYEGMFANQCEERKQFKYPPYTRLILLIVRHHDRQI
                     LQEASHQLTNSLRSVFGRRVQGPSDPLIPKIQNFYIKHILLKIEIEASPEKTKKLIRD
                     CINLSLSQPSFKSVHLQIDVDPL"
     gene            14007..15398
                     /locus_tag="PLD44_04740"
     CDS             14007..15398
                     /locus_tag="PLD44_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HOF98674.1"
                     /translation="MKNKYIDLIEQTFEFPNDEFNVIDNELWWCGIPLMDVIKQYGTP
                     LRIAYLPKIAENIQKARRMFNVAMAKVDYQGDYMYCYCTKSSHFSFVMEEVLKHDVHL
                     ETSSAFDINIMESLYEQGLLKPETYIICNGFKRPQYIENIQNLIRLGFSNVVPILDNK
                     FELDLLLKDFDKKLKIGIRIAAEEEPKFDFYTSRLGIRYNDIIPYYTKHIANNLDVEL
                     KILHFFINTGVKDTAYYWNELSKAVNLYCELKKKCPELDSLNIGGGFPIQTHLDMSYD
                     YEYMAEEIIAQIKNICTQNGVPEPNIFTEFGSYTVGESGAMLYSIVNQKKQNDRELWD
                     MIDSSFMTTLPDTWAINQRYVFLAINNWDSEYERVNLGGLTCDSEDFYNAEVHSNAIF
                     LPKMEEGREQYIGFFHTGAYQESLSGYGGIQHCLIPAPKMVIIDKDEDGEYFTKLFAK
                     EQSYKSMLKILGY"
     gene            complement(15540..16523)
                     /gene="pfkA"
                     /locus_tag="PLD44_04745"
     CDS             complement(15540..16523)
                     /gene="pfkA"
                     /locus_tag="PLD44_04745"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOF98675.1"
                     /translation="MKYEIKCIGLLTSGGDAPGMNAAIRAVTRTAIFNGIRVKAIYRG
                     YKGLITGEIKEFKTENVSNIIQQGGTIIKTARCAEFMTPEGRKTAYETMRKEEIDALI
                     VIGGDGTLTGARIFAQEFNVPIVGLPGTIDNDLWGTDVTIGYDTALNTIIDAVDKIRD
                     TASSHERLFFIEVMGRDAGFLALSSALASGAEAAIIPERETKVDQLEELIKNGFRKSK
                     NSSIVIVAESEITGGANGLAERMRREHPEYDVRVTILGHIQRGGSPTASDRILASRMG
                     VAAVDALLDEQRSIMIGIVNDEIVHVPFTKAIKFDKPVNEDLLGVIQILSI"
     gene            complement(16567..17433)
                     /locus_tag="PLD44_04750"
     CDS             complement(16567..17433)
                     /locus_tag="PLD44_04750"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOF98676.1"
                     /translation="MLNIEIDNKSGFCFGVVKAIEKAEEELEKGSTLYCLGDIVHNEE
                     EVERLQRLGLVTVNYEQYNQLHNVKVLIRAHGEPPSTYETARKNNITLIDATCPVVLK
                     LQSRIKKNYEELASEDGQIVIYGQTGHAEVKGLVGQTDGKAIVIQEEKDLDKIDFSKK
                     VHLFSQTTKDLEHYHHLIEEISKRMVKKDDFKFSDTICRQVANRIPNIINFAQQNDVI
                     LFVGGKQSSNGKVLYEHCKSINPQTYYISDCKQVDELEIQKDKKIGICGATSTPQWLM
                     DCIAKRLEERLA"
     gene            complement(17499..18185)
                     /gene="cmk"
                     /locus_tag="PLD44_04755"
     CDS             complement(17499..18185)
                     /gene="cmk"
                     /locus_tag="PLD44_04755"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOF98677.1"
                     /translation="MKKIVVAIDGFSSGGKSTMAKDLARDVGYTYIDSGAMYRAVALY
                     AMRNGWITENDIDIEALKKNIGSLKIDFKTNEHGVSETYLNGENVEKEIRTLEVANAA
                     SRVSALDFVRRELVRQQQQMGLQKGIVMDGRDIGTVVFPEAELKIFLTASPEIRAQRR
                     MEEMLARGEKVDFQKVLANIKERDERDLHRSESPLKKAPDAIVIDNSNLTRDEQRELL
                     KTLFYQKTLE"
     gene            complement(18225..19493)
                     /gene="purD"
                     /locus_tag="PLD44_04760"
     CDS             complement(18225..19493)
                     /gene="purD"
                     /locus_tag="PLD44_04760"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOF98678.1"
                     /translation="MNILLLGSGGREHALAWKIAQSSQLTNLYIAPGNPGTSTLGTNL
                     PFSADDFQSIKIAVLEHEIDWVIVGSEDPLVKGIADFFASDKQLKNVSVIGPSKIAAQ
                     LEGSKNFAKKFMLRHGIPTAKYFSVTSETIDEGIQFLQQLSSPYVLKADGLAAGKGVL
                     IIDSLEEAEQELKSMLEGKFGTASKTVVIEQFLSGIECSVFVITDGNNYKILPEAKDY
                     KRIGEGDTGLNTGGMGAVSPVSFADKYFMKKVEENIIRPTINGLREEGIVYKGFIFFG
                     LINVKGEPYVIEYNVRMGDPETEAVMLRIKSDFVDLLGGVVNENLDEKEIKIDERFAA
                     TVMLVSGGYPQAYEKGKEISGLDKVEGSIIFHAGTALKNGKLVTNGGRVMAISSYGNT
                     MKEALEISLRNAEKINFEGKYFRKDIGFDL"
     gene            19618..>19822
                     /locus_tag="PLD44_04765"
     CDS             19618..>19822
                     /locus_tag="PLD44_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98679.1"
                     /translation="MSTYEQLDALARYILPKEILDDFDIVGIEEKSGVLNSIFLKDNN
                     KIKYLSNVAESQFKSFNTIAATVY"
BASE COUNT         6207 a         3969 c         3608 g         6038 t
ORIGIN      
        1 gagcataagc gaaattggaa gttgttaatt caacttttac ctgataatcg gctattggtg
       61 ttgccggatt caaagtaatc ggtactcgat aactgaaatt ctcttccgct gatttcatcc
      121 atgctttcag tgtcatggca ttcgaaacat ttaatgttga agaattaggc acagaaacat
      181 acttgtgagc tccattaaaa ttcaatgctt tgcctgctcc ctgagctgaa acctgcaaaa
      241 taaaggaaaa attaaacaaa aacatagcca gcgaaaatgc aagcaaccaa gagccaaatg
      301 agtaaaaaaa atttttcata atgtatcaat tatttaagat tggtaatgtg taaagttttc
      361 tgattcgtat tttgatagca aaacaaaata gtaagataca aatccaactc ctttaatagc
      421 ggcaattttc ttcatttcgc tcaaaaccat ttcaataatc aaatattaac tctattacgt
      481 attaaataac aaatatatga ataaataata gaaattgtac cgttttagtc aaatttttac
      541 aataaaataa aacaaaataa ttgttttttt tcaatgtttc gaatgtgata gttaaaaacc
      601 agaaatatta gccaattcaa agggaaagtc aaagtcgcaa tgctccatag ttagagcttg
      661 tctcaattgt atttaagcag attctaacat ttgtttcaaa atgatactcc gttatatggt
      721 gttatctctt tgtttgtaat ggatatatcg agattcacaa acaactgttt tgtattttta
      781 aacattgcca tttcctccaa aactaaaatc ttttccttgt tgcagatttt gagtgtaggg
      841 ataaatgtga atttattgca gtacatatcg taaaaatcga ctaatgatag attttttaat
      901 aactcatttc taaaattatg aatgttgtca attatttatc ccgtttgata tagaaaactt
      961 tatgacttat catttaataa aatatgttat tggaaaacat tatgacatgt cgatttgttt
     1021 gtataaatat acatttcgtt cgggaaatgc aaatgattat ttaaaaattt aattattatt
     1081 ggacaaaaag tattattttg ttcttttttt attacttttg caaaacaaaa tattcaaata
     1141 tgagtaaaaa tacagaaaat atgatttttg atgaagcaat gatccaacaa ttctacgagt
     1201 cctttcctga aaaaattgaa cagataaaaa accggcttgg cagaccattg actttaacag
     1261 aaaaaatact ctatactcac ctctcggccg acgaaacatt aaaaaatttt gttcgtggca
     1321 tcgattatgt caatttctca cccgacagag tagccatgca ggacgctacg gctcaaatgg
     1381 ctctgttgca actcatgaat tcgggacgtt caaaaacggc agttccggct acagttcatt
     1441 gcgatcattt gatacaagct cacgtttcgg ctgaaaaaga tttggatacc gcaataaaaa
     1501 cgaatagcga agtgtacaat tttttacggg ctgtttccaa caatttcggt atcggttttt
     1561 gggaacccgg ttcgggaatt atccatcaag taattttaga aaattatgca tttcccggcg
     1621 gattgatgat aggtaccgat tctcatacac cgaatgccgg aggattaacc atgttggctg
     1681 tgggcgtcgg tggtgctgat gctgttgacg tcatgtcagg actgccgtgg gaattgaaaa
     1741 tgcctaaaat aatcggtgtg aaattaacgg gaaaactttc cggctggaca tcgccgaaag
     1801 atgttattct taaacttact ggcatgttaa ccgtaaaagg tgcaaccaat gccgtaatcg
     1861 aatattttgg agaaggaaca gcttcacttt cggctacggg aaaagctact atttgcaata
     1921 tgggcgcaga agttggagca accagctcta tttttccttt cgataacaaa tcggctgtct
     1981 atctcgaagc tacaggacgt aattcaattg ctgaaactgc aaaaaaattg gccgaacatt
     2041 tacaggccga ttcggaagtt gtacaacatc ccgaaaaata ttacgaccga ctgattgaaa
     2101 ttgatttaag cacattggaa cctcacatca acgggccctt tactccggat gcagcttatc
     2161 caatttcgat ttttgggaaa gtggtgagag aaaagaatta tcctcaaaaa ttggaagtcg
     2221 ggcttatcgg ttcatgtacc aattcttcct acgaggatct ttcacgagcc gtttcggtat
     2281 tgcgtcaggc aagggagaaa aatctgaaag ttcggtcgca aatgattatt aatcccggat
     2341 ccgaaaaaat aagaagtaca gccgaacgcg atggcatgtt ggatgaattt aaaagttcag
     2401 gagccataat tatggcaaat gcttgcggcc cttgcattgg gcaatggaaa cgcgacatag
     2461 atgtagcgga tcgtccaaat tccattatca cttcttttaa cagaaatttt gccaaacgaa
     2521 acgatggcaa tcccaatacc catacttttc tttcttctcc cgaaatagtg gcagctcttt
     2581 ctattgccgg cgatttgtgt ttcaatccgt tgactgacga gctggaaaac gaaaaaggag
     2641 aaaagatcaa actggatgaa ccacaaggtt ttgaacttcc tctgttggga tacgaaataa
     2701 aagaatcggg ttatatagcg ccttcggaag aaaaaatgga aatcaatatc gatcccaaat
     2761 cagaaagatt acaactattg caaccttttc cggcatggga tggcaaagat atgattgagt
     2821 taccattgct gataaaagtt aaaggcaaat gtactaccga tcatatttca atggcaggtc
     2881 cgtggcttcg tttccgggga catttggaca atatttccaa caatttgctc atgggtgccg
     2941 tcaatgcttt taatgataaa accaatgccg tactcgatcc ggagactaaa caatatatgg
     3001 cagtttcggc tctcgcacgc aaatacaagg caaaaggtac aggctctgtg gtagttgctg
     3061 aagaaaacta cggcgaaggt tcgtctcgtg aacatgcagc aatggaaccc cgctatctga
     3121 atgttaaagc catcatcgcc aaatcatttg cccgaataca tgaaacaaac ctgaaaaagc
     3181 agggtatgct ggctttaacc tttatcgaca agtccgatta cgataaaatt cgcgaagatg
     3241 atcgcattag cattcttgga ctttccgact tcaaaccggg gaaaaatttg ttggttcaac
     3301 ttcatcattc cgatggaagt gtcgaacaat ttaaagtggc acacagtttc aatgaaactc
     3361 agattgaatg gtttaaagcc gggagtgcac tgaatgccat gagaaaatga aacgttttgt
     3421 tccaaaactc aagaaaaatt attttccaaa agcacaaatt aatataagag aaacttatca
     3481 ataaaaaata gaggtaaaaa atgaaagaag aacttttaat ttacaaatta tctgagacta
     3541 tcaaaaaaac cagccaaata gacaaggaat tgtttaccaa atacaacgta aaacgtggtt
     3601 taagaaacga agatcatacg ggagtactgg tcggtttgac ccgcattggc gacgtagtag
     3661 gctacgaacg taccgaagat ggccaattaa aggcaattcc tggtaaactt tactatcgcg
     3721 gaattgatgt agaagaattg gttcatggta ttcagtccga aaatcgtctt ggttttgaag
     3781 aaactgcttt tttgctgtta tcagggtatc tgcctaccaa agacgaactg ggaactttca
     3841 agaaaatact caatgaagct atgcctcttc aacatcaggc aaccatgaac attatggcac
     3901 tgaaaggaca cgacatcatg aatattttgg cgcgctgcgt tttggaacaa tatatttacg
     3961 atgaagatcc cgacaacatt tcacgcgaca atcttattcg gcaatccatc aatctggttt
     4021 ccaaatttcc aactatcatt gcctatgctt ataacgttct tcgccatcac gaaaaaggac
     4081 gatcacttca tgtccgccac ccggatgaaa atttttcttt tgctgaaaat ttcttgttca
     4141 tgataaaagg aaaaggacaa tatacaccac tggaaattaa aattctcgac ttggctttga
     4201 ttcttcatgc cgaacacggc ggaggaaata attccacttt cagcgtacgt gtaaccagct
     4261 ctaccgaaac cgacacctat tcttccattg ctgccggcat aggttcgttg aaaggtccgc
     4321 ttcacggagg tgccaacctg aaagtaatgg gaatgatcaa agaaatgaga gaaaacatta
     4381 atgattggac tgacgctgat gaaattgatc aatatctgaa aaagttattg aacaaggaag
     4441 cttatgataa aaccggactg atttatggaa ttggtcacgc cgtttatacc atcagcgatc
     4501 cgcgtgccat attgttgaaa gatatggctc gcgaacttgc caaagaaaaa ggccttgaaa
     4561 aagaatttgc atttcaggaa ctgatagaac agcgtgccgt agaggtattt atgaattata
     4621 aaaatgggaa caacaacaag caggtttgtg taaatgtcga cttctattct ggatttgttt
     4681 acgaagccat tggaataccc aaagaagttt acacgcctat ttttgcaatg gcccgcattg
     4741 ttggctggtg tgctcatcgt atcgaagaac ttaactttac cagcaaaaga attattcgtc
     4801 ctgcttataa gaatgtcaac gaaattcagc aatttatccc tctcgaaaaa cgttaaatgc
     4861 aatagttgaa aggatttttt taaacaaaaa tcctttcggc ttttaaaaag gaaaaaatat
     4921 gaaaaaaata aaagttgagc aaccggtggt tgagttggat ggagacgaaa tgacgcgcat
     4981 tatctggcga attatcaaag agcagctcat ccttccctat ctggatattg atataaaata
     5041 ttacgattta agcatacaaa atcgcgatcg taccaacgat gaagttacaa ttgaggctgc
     5101 ccatgccata aaaaaataca atgtgggaat taaatgtgcc accataacac ccgacgaagc
     5161 aagagtgaag gaatttggat tgaaaaaaat gtggaaatcg cccaacggaa caatcagaaa
     5221 tataataggt ggaactgttt ttcgcgaacc tatcatttgt tccaacattc ccagactggt
     5281 gcctaattgg acaaaaccca tagtgattgg ccgtcatgct tttggcgatc agtacaaggc
     5341 aaccgatgta ctcatcaaag gaaagggaac actaaaaatg acttttactg ccgaaaacgg
     5401 agaaacacag gagtgggaag tttataattt tcaaggagat ggagtagcca tgagtatgta
     5461 caataccgat gaatccattt atggatttgc ccgctcgtcc tttcaggcag cactcgataa
     5521 aaaatggccc ttgtatttgt ccacaaaaaa caccattctt aaaacttatg atggccgttt
     5581 taaggatatt tttcaggaag tgtacgaaaa agaattcagg gaagcttttc aaaatgccgg
     5641 catcacttac gaacatcgtc tgattgacga tatggtagct tctgttctca aatggaatgg
     5701 cggctttgta tgggcttgca aaaactacga cggcgatgtt caatccgatt ctattgctca
     5761 aggatttggt tcactgggac tgatgtcgtc ggttctgatt acgcccgacg gaaaaaccat
     5821 agaagcagaa gccgcacatg gaacagttac acgtcattac agactttatc agcaaggaaa
     5881 agaaacttct accaatccta ttgcttccat ttttgcttgg acacgcggac tggcacatcg
     5941 cggaaaactg gatggcaata atgcactaat cgatttcgca agaaaacttg aagaagtctg
     6001 tgtgcaaact gtagaatcgg gaaaaatgac aaaagatctt gccatacttg ttcatggaga
     6061 aaaagttagc cgaaccgatt atctgagtac cgaagaattt ctgacagcat tgaaacaaaa
     6121 tttggaagca aaactttccg cttgattcaa taattttcct aaacaataaa actaaaaaaa
     6181 gctgcttgaa aaaatcaaac agcttttttt acattatgaa tcagaattac cgaatgttat
     6241 tcgataggtt tctctgaatt ttcagcttct tcagaagcag gtgtttcttc tgcttcaaca
     6301 accttttctt caaccttttc ggttttttcc ttcttactcg atttttgagg tttttctgac
     6361 ttttcttccg tttcggcagg tttttcagaa ggttcttcca cttttgtttc ctctgccacc
     6421 tttacttctg caggttcttc tttttcagct ggctcagctt ttttggattt tgtttccttt
     6481 ttggccaatt ttttagcggc ttcactttcc atctgatctt tcaaatctac caatgactga
     6541 atatctccaa aagtagtttt ttcgatagcc tgagtctgca cggttgaaga ttcttctttc
     6601 tttgtacgtt tggtaaccga tttctttgga gaagtcgaag tttcaccttc tgcactttca
     6661 gttttggcgg cacgtttcag gtcttcgtga atacgtgtgt gggaaagtac aattttcttt
     6721 gcatccttgt tgaattcgat aactttgaaa ggaagttttt cgtcaacctg tgcctgtgtt
     6781 ccgtcttcct ttaccaactg tttggttgaa gcaaatcctt ctacaccata aggcaaagca
     6841 ataacggccc ctttatcgag catttcaaca actacgccgt cgtgaatact atccacagca
     6901 aatacgcttt cgagcgcatc ccacggattt tcttccaact gtttgtgtcc caaactcaaa
     6961 cgacgatttt ccttgtcaat atcgagtact actacttcga tttcacttcc aatttgcgta
     7021 aattcagaag gatgtttgat tttctttgtc cacgacaaat cggaaatatg aatcaagcca
     7081 tctacgcctt cttcaatctc tacaaatacg ccgaaattgg taaaattgcg aactttggca
     7141 acatgtttgc tcccaaccgg ataacgttct tctatatttt cccaaggatc ggatttcagc
     7201 tgcttgatgc ccaacgacat tttacgttca tcgcggtcga gcgtcaaaat aatggcttcc
     7261 acttcgtcac caactttcaa aaagtcttgt gccgaacgaa gatgttgcga ccaactcatt
     7321 tctgatacgt gaatcaatcc ttccactccc ggagcaattt ctacaaatgc accataatcg
     7381 gtaatcacaa ccactttgcc ttttaccttg tcgccaactt ttaaattggg atccaacgca
     7441 tcccaaggat gtggagtaag ttgtttcaaa cccaaagcaa tacgtttttt ctcttcatca
     7501 aagtccagaa taacgacgtt gattttttcg tccagcgaaa caacttcact tggatgattg
     7561 atacgtcccc acgacaaatc ggtaatgtga atcaagccgt ccacacctcc caagtcgata
     7621 aatacgccat aggaagtgat attcttgaca acaccttcga gaatttgtcc tttctgcaat
     7681 ttgctgataa tttctttttt ctgttgctcc aattcttctt ctatcagagc tttgtgcgat
     7741 acgacaacat ttcggaattc ctgattgatt tttacaactt tgaattccat agttttgttg
     7801 acgaaagcat cgtaatcgcg aataggttta acatcgattt gtgaaccggg caaaaatgct
     7861 tcgataccaa acacatcgac aatcataccg cctttggtac ggcatttaat gaaaccttta
     7921 actacttcct gcgattcgag agctgcattt acgcgatccc atgctcgact aatacgtgcc
     7981 tgcttgtgtg aaagcaataa ttggcctttc ttgtcttcct gactttcaat ataaacttca
     8041 accttatcgc cgactttcag atcgggattg tatcgaaatt cactcatggg aacaacgcca
     8101 tcggatttat aaccgatatt tacaacaact tcacgtttgt tgatggaaat caccgttcct
     8161 tctaccactt ctttgtcctt aacggcattc agggttttgt cataaacatt ttccaattcc
     8221 ttttttgctt cagtagtcag ggtatttcct ttttcataag catcccagtc aaattcttca
     8281 ggggttattt cagcttgaac tggggcaact gattccttta caggttcttc aggtacagaa
     8341 acttcgggtg tttctgcaac aattggttct gctgcatgcg attctgcaac actttcttgt
     8401 tcgggagaca gaacttcttt tacctcttga gtttcttctt ttttggcttc aacagcttct
     8461 ggagaaacat tttctgccag ggcagattcg ttcttttcta aattgatttg gttttcttcc
     8521 attctaaaaa tttgtttttt tactaataaa tagtagatta gactttatgt tgacttaaaa
     8581 aaatacgggc acaaagatac aaaaagttta tgatttgatt tataattttt tacagaaact
     8641 tatttattac aggggcattt tttgaagttc tgtttcaatg ctttttgaat agcggcttca
     8701 gcaagtggtt ccatcacctg atagcctttt tgattgggat gaactccatc ttcggaaaat
     8761 tcggatttta aaccgtttct ttcatctgcc attgccgaga aataatccag ataaacaaat
     8821 ccgtttttct gacaataatc tttaatccat gcgttcagag ttacaatttt ctcagccggc
     8881 tgcaaacccc gacgccaagg ataatcatag gcaggcaata cggaggaaag aaccacctta
     8941 atattgtgaa cccgtgccaa ttctgacata gaagaaagat tgtcttcaat catttcgagt
     9001 gatgaaggcc ctgtattgcc tgcaatatcg ttggtgccgg ctaaaattac tactaccgcc
     9061 ggttgcagtt taatcacatc ctgacgaaaa cgcaaaagca tctgaggggt tgtctgaccg
     9121 ctgatacccc gattgatata ggaacgattt ttaaaaaaat cgggacgaat attaatccaa
     9181 ccttcggtga tggaattgcc caagaaaact actctttttt cacctttatc aggggctccc
     9241 aatttttcat tttctgctct gaaacgacct aaatttggcc agtcttgcgc attcaattgc
     9301 tgcatgtaaa aacacaacat agtcataatt aaaatattaa caaaatactt tgacttcata
     9361 acgataaata tttaagtaaa tggattgtaa aaaatatttt ggttatcaaa ttttacgaac
     9421 aacaactgtc gtaattttat gatttacaaa agtagttaaa aatagatata aattttctac
     9481 gattactctg aaaagtacaa cataacgatt cgatcgattt gcattaaaaa aagatttttt
     9541 tatcacaaag aatttagttc ttcataagaa ttgtggattt taccaacaaa attttctaca
     9601 tttgcaatgt tcacagtctt caacattatt tcgataaaac gacatgaaaa cacatacaaa
     9661 aaaattttat ttcccgaatt ttgagaaaat aatctgttta ttcctactat tgactggatt
     9721 tttctcaaaa actctttcgc aggactttgc caaaggagct gatgtaagct gggtaacaca
     9781 aatggaagct gccggaaaaa aattttatac tgccgaagga gtggaaacgg aatgtatgac
     9841 gttactgaaa agtttgggaa tgaataccat ccgtctccgt gtatgggtca atcctgccga
     9901 tggatggtgc aacgctcagg atgtactttt gaaagcacaa cgagcaaacg atttgggcat
     9961 gcgcattatg attgattttc attatagtga ctcttgggcc gaccccggga aacaaaccaa
    10021 acctgcagca tggtccactc tttcttttgc cgacctgaaa acagctcttt cccaacacac
    10081 taacgaagta cttactttgc tgaaaaacaa tgatataact ccggaatggg ttcaggttgg
    10141 caatgaaact tctgatggaa tgctttggga agatggaaga atttccaaaa atccacaaaa
    10201 ctacgccgag ttaaacaatg caggatatga tgccgtaaaa tccgttttcc ccaatgccaa
    10261 agttatcgtc cacttaccca atggctatag caacagtttg taccgttggt ttttcgacac
    10321 gttaaagaat tatggcggga aatgggacgt tatcggcatg tcggtttatc ccttttggta
    10381 cacgaccaaa aacgactgga gaaattgcaa tatcagttgt ttcaacaata tgaacgacat
    10441 ggtaagtcga tacggtacag aagtgatgat agtggaatgc ggtatgcaat gggatgctcc
    10501 ggaagcatgc agagattttc tttccgattt gattaacaaa accaaaaatg tttcgcaagg
    10561 aaaaggattg ggcgtattat attgggagcc gcagtgttat cccggatgga acggatatac
    10621 attgggcgct ttcgacaata caggaaaacc tactattgcc ctcgatgctt ttaaagaagt
    10681 cagtcaatcg acccatccgc tccaactaaa aaccggatgg cgttatgatg acttgcaaaa
    10741 agtcatactt ttcgataaaa cattcagtaa agttacttta tttgacagcg ccggcactgc
    10801 catacaaagt ttcgcttcta ccaatcgcat ttctatgaaa aacatgcaaa aaggaattta
    10861 tatccttcat gcagcattga aaaacaccga accactttta atcaaattca tttacaatta
    10921 aatgctgctg attattcttt gctgaaacaa tatccatcta aactttttaa acgttttttc
    10981 caacttatat tattttgttc aattctaaaa acagaaatcg gttcttttga aatacattga
    11041 agtcatattg cctttgccat tgcccaatac ttttacctat gctgttcccg aagcagatga
    11101 gaactcgata caacaaggta tgcgagttgt agtaccattt ggtaaaaaaa aattatattc
    11161 cggaattgta tattatgttc atattcttcg tccggaaacg acttatcaaa taaaagaaat
    11221 tgtcggtatt ttggatgagg aacccatttt gcgccgacct caaatcaaat tttgggaatg
    11281 gatagccaat tattatcagg cacatttggg ggaagtttat caatcggctt taccggccgg
    11341 tcttaaactg gaaagtgaaa ctcttgtacg ggtgaagccg gattttgata ccaacgtcca
    11401 tttttcagaa cgcgaaaaaa aaattctgga tgtcctttta aatcagaaaa agcccatttc
    11461 attgtcagcg ttggcaaaag atagcggaat ttcaaacgtt ttacctcaac ttaaactgct
    11521 tctggaaaaa gacatcatcg aaatcagcga acagcttacc gaaaaatttc atcccaaaac
    11581 tgaaacatac atacgtcttc gtcctgaaat tcaacaagat gaaaaacttc acgaaatttt
    11641 tgaccaacta aaatcggcaa aaaaacagtt aagtttgctg atgaactata ttgatctttc
    11701 aaagtgcctt gatccaaaaa atgtacgcga agtttcccgc aaaatacttc ttgaaaaatc
    11761 aggcatttct cccgctgttc tgaaagcgct caccgataaa aagatttttg agatctacga
    11821 gaaaattgtc ggacgtctcg acttttctga agacgaactt cagaaaccct ctattcttaa
    11881 cgaatttcaa acaaaagcac tgcaagaaat cgaaaaaaaa tttaaagaaa aaccggtggt
    11941 attgctgcat ggcgttactt cgagcgggaa aacggaactt tatattcatt tgattcaaaa
    12001 agttttaaac gaagggaaac aggcacttta cctggtgccc gaaattgcac taaccactca
    12061 acttactgcc cgactgaaaa gaattttcgg taaaaagctg ggagtttatc attcaaaatt
    12121 ttccgacgca gaacgcaccg aaatttggaa caatttgctg aacgacaaag gctatgaagt
    12181 tattataggc gttcgttcct ccattttttt gcctttccga caattaggtc tggtaatcgt
    12241 tgacgaagaa tacgaaacaa gttacaaaca atttgacccg gctcctcgct atcacgcacg
    12301 taacgctgcc attgtgctgg cttccatgca cggagcacac actttgctcg gttcggcaac
    12361 accggcaata gaatcctatt ttaacgctgt tacgggcaaa tacggactcg taaaacttga
    12421 tcggcgattt gaagaaattt cgttgcccga aatcgtggtt gtagatttga aagaagctta
    12481 tcacaaaaaa caaatgaacg gccatttttc cgatttgctg atgcatgaaa tgcagaaaac
    12541 tcttcaaaac agagaacaaa taattctttt tcaaaatcga agggggtatg cgccctatct
    12601 ggaatgtaaa agctgcaact acatacccaa atgcaaaaat tgcgatgtaa gtctaactta
    12661 tcataaaact accaatgctc tgacctgcca ttattgcgga tacactgaat cttatcctgc
    12721 ttcctgtccg gtctgcggaa caccggcggc cttctccacg cacggattcg gaacagaaaa
    12781 aattgaagat gaaataaaaa aaatctttcc cgaagcccga attagccgaa tggatctgga
    12841 taccacccgt tcaaggaaat cgtatgaaac gattatcacc gattttgaag agtataaaat
    12901 cgatattttg attggcactc aaatgattac caaaggactg gatttcaaca aagttagttt
    12961 ggtaggaatt ctaaacgcag ataatattct gaattttccc gattttcgat cacacgaacg
    13021 agcttttcaa ttgctatcac aagttagcgg tcgtgccggc agaaaggata aacagggaaa
    13081 ggtgattctg caaacctatc aaccgaaaca cccaattatt caacaagtaa tcgccaacga
    13141 ctacgaagga atgtttgcaa accagtgcga agagcgcaaa cagttcaaat atccacccta
    13201 tacacgactg attttactta tagttcgtca tcacgacaga cagatacttc aggaagcttc
    13261 tcatcaactg accaactcgc ttcggagcgt ttttggccgc agggtacagg gtcccagtga
    13321 tccgctaatt cctaaaattc aaaactttta tattaaacat attctattaa aaatagaaat
    13381 agaagcatct cctgaaaaaa caaaaaaatt gatacgagat tgcatcaatc tttccctctc
    13441 gcaaccttca ttcaaatcgg ttcatttaca aatagatgta gatccactct aaactccaac
    13501 caaatctgat tagataaaag tatttcccct ctttcatttt atttaaaata gatcattaat
    13561 tttggaattc gtaaaatgaa ttgatttaaa aaattttatc tcaatatata aaataagaat
    13621 ttaaccttat tctcaattca aaaaatgata aataatcaaa caaaattaat tggataaata
    13681 aaaaaagagc aatgtaaata acttggattt tctgaatttt atattttcat cttggcaaat
    13741 gataaaataa taaacaagtt aacctataag tataaagtta aacttttcaa aaaattttat
    13801 attaattaat tgattataaa catattagta tcatatatta aagtgttgat caagaaatga
    13861 ttttttgttc acctaatatt gccatataaa aaataagtat taattttgca cacatttagc
    13921 taaaacatca attttaactg aatcggataa tacattttat tgcaatttaa gtaagttata
    13981 tttatagaaa atcaagaaaa caagcgatga aaaacaagta tattgatctg attgaacaga
    14041 cttttgaatt ccccaatgat gaattcaatg tcattgacaa tgaactctgg tggtgtggta
    14101 ttccactgat ggatgtcatc aagcagtatg gaactccgtt gagaatagcc tacctgccga
    14161 aaatagccga gaatatacaa aaagcccgac gtatgtttaa tgtggctatg gcaaaagttg
    14221 attatcaggg agattatatg tactgttatt gtacaaaaag ttctcatttc tcttttgtaa
    14281 tggaagaagt tttgaaacat gacgtacatc tggaaacttc ttccgctttc gatatcaaca
    14341 tcatggaatc gctttatgaa caaggattac tgaagccgga aacatatatt atctgcaacg
    14401 ggttcaaacg tccacaatat atagaaaata ttcaaaatct gatacgtttg ggattttcta
    14461 atgtagtacc gatactggac aataaattcg aattggattt gctgctgaaa gatttcgata
    14521 agaaattaaa aataggaatc agaattgctg cggaagaaga accgaaattt gatttttata
    14581 cttccagact tggtattcga tacaacgaca ttattcccta ctacacaaag cacatagcaa
    14641 acaatctaga tgtagaactg aaaattttgc attttttcat caacactggt gtcaaagata
    14701 cggcatacta ttggaacgaa ttgagtaaag ccgtaaattt atactgtgaa ctcaagaaaa
    14761 aatgtcctga actcgacagt ttaaatatcg gtggaggctt cccgattcag acccacttgg
    14821 atatgtcgta cgattacgaa tacatggccg aagaaatcat agcacagata aaaaacatct
    14881 gcactcagaa cggagttccc gaaccaaata tatttaccga atttggttcc tacaccgttg
    14941 gcgaaagtgg agccatgctt tattccattg tcaatcagaa aaaacaaaac gaccgcgaat
    15001 tgtgggacat gatcgacagt tcgtttatga ctacactgcc agatacatgg gcaatcaatc
    15061 agcggtatgt atttttggct atcaacaatt gggattcaga atacgaacgc gtcaatcttg
    15121 gtggcctgac ctgcgacagt gaagactttt acaatgccga agttcattca aatgctattt
    15181 tccttccgaa aatggaagaa gggcgggaac aatatattgg atttttccat actggcgctt
    15241 atcaagaatc gttgagcgga tatggcggca tacagcattg tttgataccg gctccaaaaa
    15301 tggtaattat cgacaaggat gaagacggag agtattttac caaacttttt gccaaagaac
    15361 aaagctacaa gtcaatgttg aaaattttgg gttactgagt ttaaaaaaat gttaaataag
    15421 aaaccactga ctagataaaa tatcgcaggc agtggttttt aatttttcag ggaactacaa
    15481 tgtttggtaa aagaaaaaat aaaaaaacat tcttcaataa atttagcgaa tgaaaattgt
    15541 taaattgaca ggatttggat gacgcccaat aaatcttcat ttaccggctt gtcgaattta
    15601 atggcttttg taaacggtac atgaacaatt tcgtcgttca cgatgccaat cataatactg
    15661 cgctgctcat ccagcaaagc atctacggct gccacaccca tgcggctggc caaaattctg
    15721 tccgaagcag taggtgaacc accccgctga atatgtccca aaattgttac gcgtacatca
    15781 tattcgggat gttcacggcg cattctttca gccaagccat tggctccgcc ggtaatttca
    15841 ctttcggcaa caatcacaat gctgctgttt ttcgattttc tgaagccgtt tttaattagt
    15901 tcttccaact gatcgacttt ggtttccctt tctggaataa tggcagcctc cgcacctgaa
    15961 gccagggcgc tgcttaaagc cagaaaacct gcatcccgtc ccatgacttc tataaagaat
    16021 aaacgttcgt gggaagaagc cgtatcccga attttgtcta ctgcatcgat aatggtattc
    16081 aaggctgtat cataaccaat ggtaacatcc gttccccata aatcgttgtc tattgtcccc
    16141 ggcaagccta caatgggcac gttaaattct tgtgcaaaaa ttcgagcacc ggttaaagtt
    16201 ccatcacctc cgataacaat caacgcatca atttcttctt tcctcatagt ttcgtaagcc
    16261 gtttttcttc cttcgggtgt cataaattca gcgcacctgg ccgttttaat aatcgtgcct
    16321 ccttgctgaa taatgttgct cacgttttcg gtcttgaatt ctttgatttc tccggttatc
    16381 agtcccttgt aacctcggta aatcgctttt acacggatac cgttgaaaat tgccgtacgt
    16441 gttactgccc tgatagcagc attcattccg ggagcatcac ctccggaagt aagtaatccg
    16501 atacatttaa tttcgtattt catttctatt ttgttgatta attgattatc aaaaaactaa
    16561 taaaaatcag gctaaccttt cttccaaacg tttggcaatg caatccatca accattgtgg
    16621 tgtggaagta gccccacaaa ttccaatttt tttatccttt tgaatctcga gttcatccac
    16681 ttgtttacaa tcggaaatat aatatgtctg tggattaatg cttttgcaat gttcataaag
    16741 aacttttccg tttgagcttt gcttgccacc tacaaaaaga ataacgtcgt tctgttgtgc
    16801 aaaattaata atatttggta ttctgtttgc tacttgacgg caaatggtgt cgctaaattt
    16861 aaaatcatcc tttttcacca tgcgttttga aatttcctct atcagatgat gataatgttc
    16921 aagatctttg gttgtctgag aaaacaaatg tactttcttt gagaaatcga ttttatccaa
    16981 atctttttct tcctgtatca caatggcttt accatcggtt tgccctacca gtcctttgac
    17041 ttcagcgtgt cctgtttgtc cgtaaataac tatttgtccg tcttccgaag ccaattcttc
    17101 atagtttttc ttgatgcgcg attgcaattt cagtaccacc ggacaggtag catcaatcag
    17161 ggtaatatta ttctttctgg ctgtttcgta tgtgcttgga ggctctccgt gagcgcgaat
    17221 cagcactttc acattatgca gttggttgta ttgctcataa ttgacagtaa cgagtcccaa
    17281 tcgctgaagt cgttccactt cttcttcgtt gtgcacaata tctcccaaac agtatagagt
    17341 tgagcctttt tccaattctt cttctgcttt ttcaatggct ttaaccacgc caaagcaaaa
    17401 tccggatttg ttgtctattt cgatgttcaa aattttgaag ctttataaaa attaaaaaaa
    17461 cagatgaata acactgaaac tttaattgaa tcagcttatt attctaacgt tttttgataa
    17521 aataaagttt tgagcaactc tcgctgttcg tcacgagtca gattggaatt gtctataaca
    17581 atggcatcgg gggccttttt gagtgggctt tctgaacgat gaagatctct ttcgtcccgc
    17641 tcttttatat tggcaagcac tttttgaaaa tcaacttttt cacctctggc cagcatttct
    17701 tccattcttc gttgagctcg gatttcggga gaggcggtta aaaaaatctt cagctcggct
    17761 tcaggaaaaa ctacagttcc aatatctctt ccatccatta caattccttt ttgcaaaccc
    17821 atttgttgct gctgccgcac cagttctctc cttacaaagt ccaaagcact tactcggctg
    17881 gctgcattgg caacttccaa tgttctgatt tctttttcga cattttcgcc attgagataa
    17941 gtttcggaaa ctccatgctc attggtttta aaatcgattt ttaaagagcc gatatttttt
    18001 ttcagtgctt cgatatcaat atcattttct gttatccaac cattgcgcat ggcatacaag
    18061 gcaactgcac gatacatggc tcccgaatca atgtaagtat atcctacatc tcgtgcaaga
    18121 tcttttgcca tagtactttt accgccggaa gaaaacccat caatagcgac aacaattttt
    18181 ttcatcggga ttaattttta tttgcagcaa caaattccaa gcttttataa atcgaatccg
    18241 atatcttttc tgaaatattt tccttcaaaa ttaatttttt cggcatttct caatgatatt
    18301 tccagcgctt ctttcatggt gtttccataa gaactaatgg ccattactct gccgccattg
    18361 gttaccagtt tcccgttttt caaggcagtg ccggcatgaa aaataatact tccttccact
    18421 ttatccaaac cggaaatttc cttgcccttt tcataagctt gaggatagcc gccggaaacc
    18481 agcattactg tggctgcgaa acgctcgtca attttaattt ctttttcgtc caaattttca
    18541 tttaccacac ctcccagtaa atcaacaaaa tcggatttta ttcgcaacat gacggcttct
    18601 gtttcaggat cgcccatccg aacattatat tcgatcacgt aaggttcacc tttaacattt
    18661 atcaaaccaa aaaaaataaa ccctttatat acaattcctt cttctctcaa tccgttgata
    18721 gtgggtcgaa taatattttc ttctactttc ttcatgaaat atttatcggc gaacgaaacc
    18781 ggcgaaacag cgcccattcc tccggtattt aatccggtat cgccttcacc tattcgcttg
    18841 taatctttgg cttcgggcag aattttgtaa ttatttccat ccgttatcac aaataccgaa
    18901 cattcaattc cggaaagaaa ttgctcaatt acaacggttt tacttgctgt tccaaatttt
    18961 ccttccagca tggattttaa ttcctgttca gcttcttcca agctatcgat aatcagcacg
    19021 cctttccctg cagccagtcc gtcggctttc agtacgtaag gagaggaaag ttgctggaga
    19081 aattgtatgc cttcgtcaat agtttctgag gtaacgctga aatatttggc tgtcgggatg
    19141 ccatgtctga gcataaactt tttggcgaaa tttttactgc cttccagttg cgcagcaatt
    19201 tttgacggcc cgatcacaga tacgtttttt aattgtttgt cagaggcaaa aaaatcggca
    19261 atccctttca ccaaaggatc ttccgaccca acaattaccc aatcgatttc atgttccaac
    19321 actgctattt tgatggattg aaaatcatct gccgaaaagg ggagattggt tcccagcgtt
    19381 gacgtgccgg gatttccggg agcaatataa agatttgtaa gctgtgaact ttgagcaatt
    19441 ttccatgcca aagcatgttc acgacctcct gaacccagaa gtaatatatt catgttgtag
    19501 agttgttttt acgttgtgca aaagtaacat ttttcatatt ttcgggcaaa caaaaatggc
    19561 tcacctgtat aaatttgtag aaaaagaaaa gtatctttgt gaaaaaatac tttttatatg
    19621 tccacttacg aacagcttga tgcacttgcc cgttacattc ttccgaaaga gatattagac
    19681 gattttgata ttgttggaat tgaagaaaaa tcgggagttc tcaattcaat ttttcttaaa
    19741 gacaacaaca agataaagta tctctcaaat gtggctgaaa gccaattcaa gtcttttaat
    19801 acaatagctg ctacagttta tg
//
LOCUS       DAOSIB010000036        19515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_3046_length_19515_cov_10.400154, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000036 DAOSIB010000000
VERSION     DAOSIB010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19515
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3046_length_19515_cov_10.400154"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            462..1295
                     /locus_tag="PLD44_04770"
     CDS             462..1295
                     /locus_tag="PLD44_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98680.1"
                     /translation="MKSIQLSTVFFLSIIFLAACNSRAKQEKEADNQLQKIQQLIQQN
                     ELNAAKIAIDSFHTNFPRLVAKRRIAEAFQDTITRRECKRTIAYCDSILPFKQKEADS
                     LLLNFRLEKNEKYQDIGNYVYKTQSTEANAARTYLKAFVDENADYYLVSQYMGPKIEH
                     TSVEISNGEVFAHTDTIDITSASYLSFSDETGRWEIVTFKNEAENGVSEFISQYMNEN
                     LKVILHGKKNFSYFLQNNDKKAIKETYHLWIVKKDINRLKKEIEKAKLKIEQINRRNS
                     V"
     gene            complement(1305..2354)
                     /gene="mutY"
                     /locus_tag="PLD44_04775"
     CDS             complement(1305..2354)
                     /gene="mutY"
                     /locus_tag="PLD44_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOF98681.1"
                     /translation="MNFSDQLISWYQVHKRDLPWRNISDPYRIWISEIILQQTRVNQG
                     LDYYLRFVERFPDVETLAAAEEDEVLKYWQGLGYYSRARNLHHAARQIMQDFGGVFPT
                     TYSEVMQLKGVGAYTAAAVCSFAFNLPYAVVDGNVYRVLSRLFAIETPIDSHSGKKEF
                     DALAQQLMENSVPCLHNQAIMEFGALQCVPVSPDCSVCPLRVYCKAYEMKMVEQLPVK
                     SKKTPVKQRFFNYLVINYNHHIFLQKRTADDIWKNLYEFPLIETDHLLPIQELLENEF
                     FCQLFQGLEQVKIINISKPVHHLLTHRRISAQFITLQIAEKNEALHQFIEIPNAEIGD
                     FAVSRLVEMYLKKEI"
     assembly_gap    2502..2601
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2602..4634)
                     /gene="metG"
                     /locus_tag="PLD44_04780"
     CDS             complement(<2602..4634)
                     /gene="metG"
                     /locus_tag="PLD44_04780"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOF98682.1"
                     /translation="MKKFKRTLVTSALPYANGPVHIGHLAGVYVPADIYVRYLRLKGE
                     EVIFIGGSDEHGVPITIQAKKEGISPQEIVDRYHFLIKKSFEDFGISFDIYSRTTSKI
                     HHELASDIFKTIANKGGFIEKTSEQYYDEEAGQFLADRYIVGKCPHCGNENAYGDQCE
                     ACGSSLNPTDLINPRSAITGNIPVLRETKHWYLPLDQHEEWLRHWILEEHKEWKPNVY
                     GQCKSWLDMGLQPRAVSRDLDWGIPVPLERAEGKVLYVWFDAPIGYISNTKELLPESW
                     EKWWKSEDTRLIHFIGKDNIVFHCIIFPTILKLEGTYILPDNVPANEFLNLEGDKIST
                     SRNWAVWLNEYLEDFPDKQDTLRYVLTANAPETKDNDFTWKDFQARNNNELVAIYGNF
                     VNRTLVLTQNYFDGKVPVRNELHEIDLQTINELSDIKGNVEKLLDNFKFRDAQKEAMN
                     LARLGNKYLADTEPWKIVKTDKDRVATILNLSLQIAANLSIVFEPFLPFSSEKLRDLL
                     NLETFDWNNLGSIELLKPQLQLKKPVLLFDKIEDSAIEYQVQKLLNTKKKNELSAFKP
                     KDVKSNISYENFEKLDIRVGTVLACQKVPKADKLLQFKIDDGLGGRTIVSGIAQYYPE
                     PEKLIGQQVCFIANLEPRKLKGIVSEGMLLSAEDADGRITLIQPQTTVVNGAEV"
     gene            5051..7393
                     /locus_tag="PLD44_04785"
     CDS             5051..7393
                     /locus_tag="PLD44_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOF98683.1"
                     /translation="MNMQLSILKNLRFSGVFLISIFSVFSSYSASRYDLIPYPQELKP
                     QSGEFILNKKTVILCPFDQPQIVEIAQQFSDHFALVAGIKLPLRDYQTSSTNKNFILF
                     NTGSLTNENPEAYTLKISTSSVQITGNTSAGVFYGLQTLYQLFPCEVYASKESEKIKK
                     WSVPCVEITDFPRFSYRGLHLDVSRHFFPVDFIKKYIDLMAIYKFNYFHWHLTDDQGW
                     RIEIKKYPRLTEIGSKRNETLVGHYYESFPQQFDGKPYGGYYTQAEAKEIVAYAKKRG
                     ITVIPEIEMPGHAQAAIAAYPFLSCTQDTTIKVATKWGIFKDVFCTRDTVFNFLEDVL
                     SEIMEIFPSKYIHIGGDECPKDRWKECSYCQSLIKKLNLKDEHQLQSYFTQRIEKFIN
                     EHGRQIIGWDEILEGGLAPNATVMSWRGKIGGIEAARLSHDVIMTPTEYCYLDYYQAD
                     PEFEPLAIGGFVPLSKVYNFEPIPDELTPAEAKFILGAQANLWTEYILDEKQVEYMAY
                     PRATALSEVVWSNKESKNWENFKERLSTHFQRFEKLGVNASHAFYEPQFNAQVTENNK
                     LKITLSCDHPKAKIYFSLNEKNPDQHYSKPLIIDSTTIVSVATFVDGKKASRTLQKTF
                     IVSKLTNLICQPDSNVSYHQQSIFKLTNGKLGNIKTTSEWARLISNEDYLLNIDLQKN
                     QIIQRFTMGILKAPAFCGQIPSEIILYGSKDGINYQPLEEQKFSSSFTGKYEIIRPVF
                     NFPPVEVRYLKIVLKNPRFCIENLENEKNNSIFIDEIAVW"
     gene            complement(8397..9314)
                     /gene="metA"
                     /locus_tag="PLD44_04790"
     CDS             complement(8397..9314)
                     /gene="metA"
                     /locus_tag="PLD44_04790"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HOF98684.1"
                     /translation="MPLNIPKSMPAVEILRQENIFVMDSERASSQEIRPLKIVILNLM
                     PLKITTETDLIRLLSNSPLQIEIDFLQLKSHTPKNTPIEHMMAFYKTFDAIEASNYDG
                     MIITGAPVEQLEYEEVTYWPEVQEIFDWSRKHVTSTMFICWAAQAGLYHFYGIPKYPL
                     EKKMFGVFEHYIHNPQNPIFRGFDDIFYVPHSRYTEVRAEDIKKNPELILLSESDESG
                     VYMVMGRNGREFFITGHSEYSATTLDNEYKRDLAKGLPIDMPKNYYRDNNPANEPLVR
                     WRSHANLLFTNWLNYFVYQETPYDLSKIH"
     gene            complement(9397..10200)
                     /locus_tag="PLD44_04795"
     CDS             complement(9397..10200)
                     /locus_tag="PLD44_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOF98685.1"
                     /translation="MRKGFRITIYFVAVLLLMAGAWAVFHEKPVHDLPAMLKSGKLYV
                     VTDSSRMGFRLEGDSVYGFQYEIVKTFADQLGLTLVITPENNFETCLKGLTNGDYDIY
                     AGFIPVTTEWMNDIAFSDPLNTSRLVLVQKNFPDSLKRLMKNSQQDLAGDTIFVPKMS
                     PHKLRLKHLSDEIADTIHIVEVPNVNLEQLVEWVAQGKIRQTVCDEREARFLKLTYPQ
                     IDISLPLGFDQHYAWMVDKESKLLLQQLNDFLKDFIGSSAYWNIYRKYY"
     gene            complement(10250..12109)
                     /locus_tag="PLD44_04800"
     CDS             complement(10250..12109)
                     /locus_tag="PLD44_04800"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HOF98686.1"
                     /translation="MFILFIVTALSVWAINPPSIRYVKTWKVSDPLGMADSVQVDTLH
                     LNFQDENPIDRFSIANSYNGNLGSPIQSKLYFSRPESNDFIFSDAFYPYITDMQSAVF
                     YNTKTPFTKIVYNTGGSNYRQEDQIKFLFTFNANKRLNFGTTLDYIYSVGEYANQAAK
                     RFTGSLFGSYDGKHYSAVGLAAVNNMFNHENGGISDSSYIYEPIGVKTQDIPVNIHGY
                     STYRKNVFFYHQKYTIGFDQIIRISPDSVRKEYVPVTHFGHTIKYEDPRKRYYEANVE
                     KDFYKNTYLPFTQTNDTAALQTLTNTVSVGIAEEFNKWMRFGLTGFVENELQRFTYNV
                     DSLIQKKNESNTKVGGILSKQRGERFRYNILGDIYLLGYKQGDFSIKGQVNGFFKAWK
                     DSIVLSANGFVRSDEPSYFLNYYQSNHFRWENNFDKIYRTNAGGTFSIPTRKFSVNVS
                     VENLTNYVYFNSEALPEQYDGNIQVIAANLKKDFHVGNFVLENNVVYQLSSQERVLPL
                     PTLTLFHNLYFKNMLFNVLSIQLGANVRYHTAYYAPCYMPAIAQFYTQSETKVGNFPV
                     VNVYANFHLKQARFFVEYFHVNQLFMNHAYFSMPNYPINPAIFKIGVATNFYN"
     gene            complement(12293..13381)
                     /locus_tag="PLD44_04805"
     CDS             complement(12293..13381)
                     /locus_tag="PLD44_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOF98687.1"
                     /translation="MKMNNFFKYFSFYALIFGFFLFSSLNLWASNEKKINVKIACVAF
                     YNQENLFDTIDNENVNDYEFTPNGPNRWNSMKYKSKLEKMSYAISQIGLDVTPDGAAI
                     IGVSEIENRGVLEDLVSQPSLKKRSYEIVHYDGPDRRGVDVALLYNPRYFIVSNSKSY
                     RLHTADTTFRTRDQLMVSGYLFGEKIHVIVNHWPSRSGGEMRSRPKRNAAAALTRSIV
                     DSLYRVDKNAKIIVMGDLNDDPFNESVAKVLGAKKNASEVKETELYNPFWKILDRGIG
                     SLAYNDQWNLFDQIIISAPLLNAPPSELKFWKAEVFNRDFLIQQEGRYKGNPWRTFAG
                     GVWLNGFSDHFPTLIYLIKEVDSQANEN"
     gene            13769..16498
                     /locus_tag="PLD44_04810"
     CDS             13769..16498
                     /locus_tag="PLD44_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOF98688.1"
                     /translation="MKKLFLILFVFFLCIPLLMAETSIKGTVKSADTQNPIPGVIVSL
                     IGQNISTQTNAEGQFSLRFIEAGDQEIRISKIGYFTQIKLIHIEPDKENDLGTFYLRE
                     DVQQQTKQEVVLQLSENQIDEDEGRATQNISAALSSKGDVYVSQSSFSFSPMRFRTRG
                     YDDNFESTYINGVYFNSLERGGFNYSSLGGLNDAMRNQDYTYGFAPNSFSYGNLGGTT
                     NINTKASNYAAGTKASVAYTNRSYKLRGQATYATGLMQNGWAFAGSAVVRWADKGIVD
                     GTFYNSWGYFFSAEKILNKQHSLSFVTFGSPTQRAQQGAVTQEVFDLAGSIYYNPYWG
                     YQEGKIRNSRIVKSFDPTAILSHEYKISNHQLLRTGLAFHYSLYSNSALNFYNAPDPR
                     PDYYRNLPSFVSDDSLKNVIANQWKSDTNVSQVNWSALYQANYKNNVINPNGTARYIV
                     ERRHNNLMEGALNSTYTQQVNKQLKLIAGIEGKMSKGMHYKTLDDLLGGNQWIDIDQF
                     SERDFPSNPDIIQNDLRNRNAVIKEGDTFGYNYDINMAYANGFIQNEWILPQFDIYYA
                     AKLTYTQFYRYGHMQNGRAPENSYGKSKTWYFVDPSFKAGFTYKIDGRNRLSFNGLIE
                     SRAPLASQAYLSERIKDMLIPYLQSQDIRSADINYNFTYSTIRGRIGVFQTNLYNTSD
                     MLGYYDDEYRTFINHVLAKSDKVYKGVELGTTVKINSNFSVSLAGTYADYHYANNAIG
                     VKSPENGAFADVAETVLTKGLKIAAGPQAAGNITIDYFHPKMWFADVTLNYFDNNYLD
                     FAPNRFTENNMAKYTTPEMMAALGTQEKLKGGFLLDASVGKLIYLKHRRSLNFNLSAS
                     NILNNTKMITGGYQQARLPLSNGAIDPTGLNRFPNKYYYAWGFNVFFNIGYKF"
     gene            16543..17529
                     /locus_tag="PLD44_04815"
     CDS             16543..17529
                     /locus_tag="PLD44_04815"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HOF98689.1"
                     /translation="MKNFKIGGISILLAAIFTSCAETDFDAIPVNKLMGDSLTTTYTI
                     EQLKNNFLTLNDAYSDTVNYKGGLYTADPIQAESDIVISGYITSTDVDGNVYKYFVVQ
                     EPGANGQAIKVSIDASGLSAIYPLGQKVWIRCNGLYIGRYGEAEQIGSRYVNKERFKV
                     KKSTGDTIYRIEPGRMPFLIANEHIHAYGMPDPTVIKPDTMTIAQIKASNHTKLVNKL
                     VCIKNAFFTGKSEGDNLTDSELIFAPSTNGAGYPQLRDINDGTGEISIATSEYAKFAN
                     YPLPASTYRGDITVIVGWYRNYTNAAGAWQLTLRTINDLGKGFESYKAKIQK"
     gene            17556..19043
                     /locus_tag="PLD44_04820"
     CDS             17556..19043
                     /locus_tag="PLD44_04820"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6359 domain-containing protein"
                     /protein_id="HOF98690.1"
                     /translation="MKKFNSKIVFTALAVLFTASCTNVEDVYIEKPTSDVIYSETFQN
                     GLGDFKAVNVFGDQVWTYNSSGYAIMTGYANSTNYANEDWLISPEIDLTNIASAYFSF
                     DYVMRYSNDPANDATVWILADQAYSPDTLPNVSQWVKLDLKPFVDPGSWTFSNSNDIS
                     LNDFVGKKIRIAFRYISSATKAGTWEIKNFTVKKGTAKVVESNDGSEEKPFTVKEAIQ
                     NQAGASAWVKGYIVGYIWSGTQNNYVFSADTCTQATNIILADTNDLSALYVSKILAVQ
                     LPAGVVRDSLNLKDHKDKFGKNVTLYGSLTKYFGIGGLKSVSYYKLEDGTSGGTKPVK
                     AIFSETFANSKGDFTIVDVTKVSVLPNIWAHDASYKCMKATAYVNSTNTNYESESWLI
                     SPTINLSGVTNPVMVFDYVTRYFANPATDATIQVSENYQGGDPRSTTWTQIPYPFTNM
                     SSWTFVTTNPIDLSAFANKNIRIAFKYTSTSAKAGTWEIKNLVIY"
     gene            19194..19349
                     /locus_tag="PLD44_04825"
     CDS             19194..19349
                     /locus_tag="PLD44_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98691.1"
                     /translation="MARVLRVAANLKEAISKADVLTNRNLIGGSNERATEPWIGNARN
                     STVIQEE"
BASE COUNT         6420 a         3641 c         3500 g         5854 t
ORIGIN      
        1 aatatatttg agtaaatcaa catttaaaaa ccttatgaat atcattaaat tcagttcaca
       61 attctacaac aaaaagttat ttattgtaca ttctaaggat taaagatatg ctttcggaat
      121 aatttattcc acatgtagta ttgtaaacca ttacccgatg caaaataaac tctgttagga
      181 atataaacca tgtcctcatt gtcaaccctt tcgttcttaa acattaatgg aaggcagtaa
      241 attacctttt agataccgat attttaactt tgataacatt aaaaagtata caccttcttt
      301 tttatgtaca atttgtttta tacatttgta tgttttgtat tttcaattga attttccaat
      361 aacattatga aacccacatg tattgcatac ataaacacga atgaatataa tattcagaca
      421 tgtgggtttc atcagacctt taaaaaataa attatattct gatgaaatct attcaactct
      481 caacagtatt tttcttgtcg ataatttttc tggcagcatg caatagcagg gcgaagcaag
      541 aaaaagaagc cgataaccaa cttcagaaaa tccaacaact cattcagcaa aatgaattaa
      601 atgctgcaaa aattgccatc gacagttttc acacaaattt tccgcgtttg gttgccaaaa
      661 gacgaatagc cgaagctttt caggatacta ttacacggag agaatgcaaa agaacaattg
      721 cttattgcga cagtattttg ccttttaaac agaaggaagc cgattctctg ctgctaaatt
      781 ttcggttgga aaaaaacgaa aaatatcagg acattggaaa ttacgtttac aaaacccaat
      841 cgactgaagc aaatgcagct cgtacttatt tgaaagcatt tgtagatgaa aatgccgatt
      901 attatttggt gagtcagtat atgggaccaa aaattgagca tacttcggtt gaaatatcca
      961 acggcgaagt ttttgcacat accgacacca tcgacattac cagtgcttct tatttgagct
     1021 tttcggatga aaccggacgt tgggaaattg taactttcaa aaacgaagct gaaaatgggg
     1081 tttcagaatt tatttcgcaa tatatgaatg aaaatctgaa agtaattctg cacgggaaaa
     1141 agaatttcag ctactttctt cagaacaacg ataaaaaagc cattaaggaa acttatcatt
     1201 tatggatagt aaaaaaggat attaaccgct taaaaaagga aattgagaaa gcaaaactga
     1261 aaatagagca aattaacagg aggaattcgg tttaggcgga aaaatcaaat ttcttttttc
     1321 aaatacattt ctaccagccg cgaaacagca aaatcgccaa tttctgcgtt tggaatttca
     1381 ataaattgat gcagtgcctc atttttttcg gcaatctgta aagtaatgaa ctgagccgaa
     1441 atgcgccgat gcgtcaaaag gtgatgaaca ggttttgaaa tgtttataat ttttacttgt
     1501 tccagtccct gaaacaactg gcaaaaaaac tcattttcaa gcaattcctg aatcggcaac
     1561 aaatggtctg tttcgatcaa tggaaattcg tacaaatttt tccaaatatc gtccgccgtt
     1621 cttttctgca aaaaaatatg atggttataa ttgataacca gataattaaa aaatcgttgt
     1681 ttgacaggtg tttttttaga ttttaccggc aactgttcta ccattttcat ctcataagct
     1741 ttgcaataaa cccgtagagg acaaaccgaa caatcgggag aaacgggcac acactgcaat
     1801 gcaccaaatt ccataatggc ctgattgtga agacaaggaa cagaattttc catcaattgc
     1861 tgagcaagag catcaaattc ctttttacct gaatggctgt cgataggcgt ttcgatagca
     1921 aaaaggcgtg aaagaacacg atagacattt ccatccacca cagcatacgg aagattgaag
     1981 gcaaaagaac aaacagccgc tgcggtataa gctccaacac cttttaattg cataacttcc
     2041 gaataagtgg ttggaaaaac gccgccaaaa tcctgcatga tttgtcgcgc agcgtgatgc
     2101 aaattgcggg cacgcgaata ataacccaaa ccttgccagt atttcaaaac ttcgtcttct
     2161 tcagcggcag ccaacgtttc aacatccggg aatcgttcca caaacctcaa ataataatcc
     2221 aatccctgat tgacacgcgt ttgctgcaaa atgatttcgg aaatccatat acgatatgga
     2281 tcagaaatgt ttcgccatgg caaatcacgt ttatgaacct gataccagct gataagttga
     2341 tcggaaaaat tcatgttttc aaaaaattca aagaaaaact ttcaactctg caaaaaaaac
     2401 aaaaaatagc ctcgattgaa atttacttta tatcaaatgc tatttacgag gttttgcccc
     2461 aaacctcact ataagaaaaa tggacgttca ttaacaaaaa annnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nacttctgcc ccattcacta ctgttgtttg aggttggatc
     2641 aaagttattc tgccatcagc atcttcggcc gagagaagca ttccttcgct gacaattcct
     2701 tttaattttc gtggttcaag gttggcaata aagcaaactt gctgtccaat taacttttca
     2761 ggttcaggat aatactgagc gattccggaa acgatagtac gaccgcccaa accatcatca
     2821 atcttgaact gtagcagttt gtcggctttc ggaacttttt gacaagccaa cactgtgccg
     2881 acgcgaatat cgagtttttc aaagttttcg tacgaaatgt tggattttac gtctttaggt
     2941 ttaaacgccg aaagttcgtt ctttttcttc gtatttagca gtttctgaac ctgatattcg
     3001 atagcactgt cttcaatttt gtcaaaaaga agcaccggct ttttcaattg aagctgaggt
     3061 ttcagtaact caatgctgcc caaattattc cagtcgaaag tttccaaatt caacaaatcc
     3121 cttaattttt ccgaactgaa tggcaaaaac ggttcaaaga cgatggaaag attggctgca
     3181 atctgcaaac tcaaattcaa aatagtagca actctatcct tatctgtttt tacaattttc
     3241 cagggttcgg tgtcggccag atatttgtta ccaagccggg caagattcat ggcttctttt
     3301 tgtgcatcgc gaaatttgaa attatcgagc aatttttcga catttccctt tatatcagag
     3361 agttcgttaa ttgtctgtaa atcaatttca tgcaattcat ttcgaactgg aacttttcca
     3421 tcaaaataat tttgtgtcaa cacaagagtt cgattcacaa aattaccgta aatagctacc
     3481 aattcattgt tgtttcgagc ctgaaagtct ttccatgtga agtcgttgtc tttcgtttcg
     3541 ggagcgttgg cagtcagcac ataacgcaaa gtatcctgtt tatcgggaaa atcttccaga
     3601 tattcgttca gccaaactgc ccaattgcgg gaagttgaaa ttttgtcgcc ttcaaggttt
     3661 aaaaattcgt tggccggtac attatccggt aaaatataag ttccttcgag ttttaaaatt
     3721 gttgggaaaa taatacaatg aaaaacgata ttgtcttttc caataaaatg aattaatcgg
     3781 gtatcttcac ttttccacca tttttcccag ctttcgggca atagttcttt ggtgttggaa
     3841 atataaccaa tgggagcatc aaaccataca tatagtactt ttccttcggc cctttcgagt
     3901 ggaacgggaa taccccaatc caaatcgcga cttacggcac gcggttgtaa gcccatatcc
     3961 agccaacttt tgcattgtcc gtaaacattt ggtttccatt ctttgtgctc ttccagaatc
     4021 caatgccgaa gccattcttc atgctgatcg agaggcaaat accaatgttt tgtttcacgc
     4081 agcacaggaa tatttcccgt aattgctgag cgcggattaa ttaaatcagt aggattgagc
     4141 gaggaaccgc aagcttcaca ctgatcaccg taagcatttt cgtttccgca atgcggacat
     4201 tttcccacaa tatatcggtc ggcaagaaat tgaccggctt cttcatcgta atactgttca
     4261 ctcgtttttt ctatgaaacc acctttattg gcaatggttt taaaaatatc cgaagccaat
     4321 tcatgatgaa tttttgaagt ggtacgagaa taaatatcga aagaaattcc aaaatcttca
     4381 aatgattttt taatcagaaa atggtaacga tccacaattt cctgcggaga aatgccttct
     4441 ttttttgcct gtatggtaat gggaacacca tgttcatcgg aaccgccaat aaaaatcact
     4501 tcttctcctt tcaaacggag ataccttaca taaatatcag ccggaacata tacacctgcc
     4561 aaatggccga tatgtaccgg gccattggca taaggcaaag ccgaagtaac cagcgtacgt
     4621 ttaaattttt tcatttaaat caattaaaaa tttgtcttga agagctttaa aagaatgtgc
     4681 aaaattatat gaaaatggct ttatttccaa acgcatgaaa ataaaaatca aggtcgcaaa
     4741 actttaaagg cttaccgtag cgtgctttcg agcacaaatg ttacctttgc aagttggttt
     4801 ttttaataac caatgaaata aactaatatg attattcgca atgattccta ttgtaaattc
     4861 ttgagttgaa ataggagtta taacataaat aaaaacatat atataattga taattaaata
     4921 tttaacaata aaaaacattt ggtatatatt aaattttttc aaatccgctt ttcgaattgc
     4981 atcaaataat tttcttttga aagaataaaa atgtaaattt gaaaaatctt ttttttaaat
     5041 tttaataacc atgaatatgc aactttcaat tttgaaaaat ctccgttttt caggagtatt
     5101 tttaataagt attttttctg tatttagttc atactcagct tctcgttatg atttgattcc
     5161 ttatccacaa gaactaaaac ctcaatctgg tgaatttatt ttaaataaga aaaccgtcat
     5221 tctttgtcct tttgatcagc cccaaatcgt tgaaattgct caacagtttt ctgatcattt
     5281 tgctctggtt gctggcataa aattaccttt acgggattat caaacgtctt ctacaaataa
     5341 aaatttcatt ttatttaata caggttcatt aacaaatgaa aatcctgaag cttacacctt
     5401 aaaaatttct acttcttcgg ttcaaattac agggaacaca tcggctggcg ttttttacgg
     5461 cttacaaaca ttatatcagc ttttcccgtg cgaagtttat gcttcaaaag aaagcgaaaa
     5521 aataaaaaaa tggtcagttc cctgtgttga aattacagat tttccgcgtt tcagttacag
     5581 aggacttcat ctcgatgttt ctcgtcattt ctttcctgta gattttataa aaaaatatat
     5641 cgacttaatg gctatttata agttcaacta ttttcattgg catcttactg atgatcaggg
     5701 ttggcgaata gaaataaaaa aatatcctcg acttacagaa attggttcta aaagaaatga
     5761 aacattggtt gggcattatt atgagtcgtt tccacaacaa tttgatggaa aaccttatgg
     5821 cggttattat acccaagcag aagctaaaga aattgttgct tatgctaaaa aacgaggcat
     5881 tactgtaatt cctgaaatag aaatgcccgg gcatgctcaa gctgctattg ctgcttatcc
     5941 ttttttgtcg tgtacccaag acacaactat taaagttgcc actaaatggg gtatatttaa
     6001 agatgtattt tgtacacgtg atactgtttt taatttttta gaagatgtat tatctgaaat
     6061 tatggagatt tttccttcaa aatatattca cattggagga gatgaatgtc cgaaagatag
     6121 atggaaggaa tgttcctatt gtcaatcgtt gataaaaaag ttgaatttga aagatgagca
     6181 tcaattacaa agttatttta ctcaacggat tgaaaaattt attaatgaac atggtcgaca
     6241 aatcattggt tgggatgaaa ttttagaagg tggtttagct cctaatgcta cagttatgtc
     6301 atggcgagga aaaattggtg gtattgaagc tgctcgcctt tcgcacgatg taattatgac
     6361 accaaccgaa tactgttatt tagattatta tcaggctgat cctgaatttg aaccactcgc
     6421 tattggtggt tttgttcctt tatcaaaagt atataatttt gaacctattc cagatgagtt
     6481 gactcccgca gaagcaaaat ttatattggg agctcaagcc aatttgtgga cagaatacat
     6541 ccttgatgaa aaacaggtgg aatatatggc ttatccacgt gcaactgcat tatcagaagt
     6601 agtatggagc aacaaggaat caaaaaattg ggaaaatttc aaagaacgtt tgtcaactca
     6661 ttttcaacga tttgaaaaac taggagtgaa tgcctctcat gcattttatg aacctcaatt
     6721 taatgctcaa gtaacggaaa ataataaatt aaaaattact ttaagttgtg atcatccaaa
     6781 agcaaaaatt tatttttcat taaatgaaaa aaaccccgat cagcattact caaaaccgtt
     6841 aataattgat tcaactacta ttgtatcagt tgctactttt gttgatggta agaaagcaag
     6901 cagaacttta caaaaaactt tcatagttag caaattaaca aatttaattt gtcaacctga
     6961 ctccaatgtt tcttatcatc aacaaagtat ttttaaatta accaatggaa aattaggaaa
     7021 tataaagacc acttctgaat gggcgagatt gatatcaaat gaagattatt tattaaatat
     7081 cgatttacaa aagaaccaaa ttattcaacg ttttacgatg gggatattga aagcacctgc
     7141 tttttgcgga caaattcctt ctgaaattat tctttatggg tcaaaagatg gaattaatta
     7201 tcaacctctt gaagagcaaa aattctcatc ttcttttact ggaaaatatg aaattatacg
     7261 accagtattt aattttcctc cagttgaggt tcgttattta aaaatagtgt tgaaaaatcc
     7321 tcgtttttgt attgaaaatc ttgaaaatga aaaaaataat tctattttca tagatgagat
     7381 tgctgtatgg taaacagttt atcagagaaa tttttctgta attgttctga atcactgttg
     7441 acctacaaaa aaaattaagt aaaatggaag ggacaagttg gaagcctcgt ctggggtaga
     7501 aaatttgtat ttcaaactca aattaacagt cctggttaaa gatacaacga caaattttgt
     7561 aggtcagccg aaaagaaaaa gtcagttttt gagaaatttt aagcattcga ttctactact
     7621 atcacacttt tcccgaaata atgaaacgga taggcggaaa tcccaaaggt gaagggaaaa
     7681 ggaaaggagg gttgaaagta cacatgctga ccgatgacat agtcagacgc cttttttagt
     7741 gaaaattagt aacgcaaaga ggcacgacaa gaagtttttg acatttttgc atccttgatg
     7801 ggaagtacat tagtttacga taaagcctat aattactatc tccaatttgc tgcctggaga
     7861 gagaaagaag taaattttgt ttgtcgtctg aaagaccatg tcaagaaaca agtacaagag
     7921 gttttgttta agaaaaagtg ttcaaaagaa gcatttggtg tttacagggt agggcatata
     7981 cacttggatt ataaggaaaa caaagaaaga aaaaaacact ttgtttgcgt ttggttataa
     8041 taaagatgag aaagggcaaa aatacaagtt ataaccaata attgagaaat aagagctgaa
     8101 gaagaaactt tgaaatacca ttatcgttgg acaatagagt tgacatttaa aaaaactgaa
     8161 acaaaatctt caattgcatt ttttctattc tgagacaaaa aatgttatgt aaaatattaa
     8221 aacaacaaaa aatttaatgc ctcttttcaa aaaaacctga ttttttctcc cttttgaaaa
     8281 aatcaggttt taagagggga ttcaaaattg aaacaaataa aaaatacaat tagttaatta
     8341 tcaattattt ttttaaaaaa tcttgtctta tactgaatta aacaccaaat atttctttaa
     8401 tggatttttg aaaggtcgta gggtgtttcc tgataaacga aataattcaa ccagttagta
     8461 aataaaagat tggcatgact tctccatcga accagcggtt cattggcagg attattgtcg
     8521 cgatagtagt tttttggcat atcaataggc aaaccttttg ccaaatctct tttgtattca
     8581 ttatccagtg tggtagctga gtattccgaa tgtcccgtaa tgaaaaactc tcttccgttg
     8641 cgtcccatta ccatataaac acccgattcg tccgattcgg aaagcaatat cagttcagga
     8701 ttttttttga tatcctccgc cctgacttcg gtataacggc tgtggggcac ataaaaaatg
     8761 tcgtcgaacc cgcgaaaaat gggattttgc ggattatgaa tataatgctc gaaaacgcca
     8821 aacattttct tttccaacgg atattttgga ataccataaa aatgatacaa tccggcttgg
     8881 gctgcccagc aaataaacat ggttgaagtt acatgcttgc gcgaccagtc aaatatctct
     8941 tgaacttcag gccaataagt aacttcttcg tattcgagct gttctaccgg agcccccgta
     9001 ataatcatcc catcgtaatt acttgcttca atggcatcaa aagttttgta aaaagccatc
     9061 atgtgttcaa taggcgtatt cttcggagtg tgtgatttca attgaagaaa atctatttca
     9121 atctgcaggg gagaattgga aagcaagcga atcaaatcgg tttcggttgt aattttgagc
     9181 ggcatcaggt tcagaattac aattttcagt ggcctgattt cttggctcga tgccctttcg
     9241 gaatccatca caaaaatatt ttcctgcctt aatatttcaa cagccggcat cgattttggt
     9301 atatttaacg gcatagtcgt gaaaagtttt tatattctgt tttaatttga atcaaataaa
     9361 tagctgaaaa gcaagctctg tccattaaaa ttttattcag taatactttc ggtagatatt
     9421 ccaataggcc gaacttccaa taaaatcttt aagaaaatcg ttcaattgct gaagcagcag
     9481 tttcgattcc ttatccacca tccatgcata atgctgatcg aacccaagcg gcaaggaaat
     9541 atcaatctgc ggataggtta atttcagaaa ccgggcttct ctttcatcgc aaactgtttg
     9601 ccttattttt ccctgcgcaa cccattcgac caactgctcc aaattaacat taggaacttc
     9661 gacaatatga atggtatcgg ctatttcgtc cgaaagatgt ttcaatcgca acttgtgcgg
     9721 cgacattttc ggaacaaata tcgtatcgcc ggccaaatct tgctgagaat ttttcatcaa
     9781 tcgtttcaaa gaatccggaa aatttttttg taccaaaacc aaacgggaag tattgagcgg
     9841 atcggaaaaa gcaatatcgt tcatccattc ggtggttacg ggaataaaac cggcgtaaat
     9901 atcgtaatcg ccatttgtca atcctttcag acaagtttca aaattatttt caggggtaat
     9961 aaccagtgtc aaacccaact gatcggcaaa agttttaact atttcgtact gaaatccgta
    10021 aacgctgtcg ccttccagac gaaaacccat ccggctactg tcggtaacaa cataaagttt
    10081 ccccgatttc agcatggcag gcaaatcgtg cacgggcttc tcatgaaaaa cagcccatgc
    10141 gccagccatc aagagcagca ctgccacaaa ataaattgtt attctaaaac cctttctcat
    10201 gatttaaaaa atgttgaact acaaatctat cattttgttt acagcaaaat caattgtaaa
    10261 aattggtagc cacgccaatt ttaaatatgg cagggttaat gggataattc ggcatagaga
    10321 aataggcatg attcataaac aattgattca catggaaata ttctacaaag aaacgagcct
    10381 gtttcaaatg aaagtttgca tacacattta ccactggaaa attcccgact ttggtttccg
    10441 attgcgtata gaattgagct atggcaggca tgtagcaggg agcataatag gcagtatggt
    10501 aacgaacatt tgcacctaat tgaatactca aaacattaaa caacatgttt ttaaagtaaa
    10561 gattatgaaa cagggtcaaa gtcggcaaag gtaaaacacg ttcttgcgaa ctcaattgat
    10621 acaccacatt gttttccaaa acaaaatttc caacatgaaa atcctttttc aaattggctg
    10681 caattacctg aatatttcca tcatattgct ccggcaatgc ttcggaattg aaatatacat
    10741 aattggtcag attttctacc gaaacattca ctgaaaattt tcgagtgggg atggaaaaag
    10801 tgccaccggc attggttcga tatattttgt caaaattgtt ttcccaccgg aaatggttcg
    10861 attgataata atttaaaaaa taagaaggtt catcgctgcg aacaaaaccg ttagccgaaa
    10921 gcacaatact gtctttccat gctttgaaaa atccgtttac ctgtcccttt atcgaaaaat
    10981 cgccctgttt atacccgagc aaataaatat cgcccaatat gttgtatcta aatctttcgc
    11041 ctcgttgttt ggacaaaata cctcccactt ttgtgttcga ttcgtttttc ttttggatca
    11101 gcgaatcgac attgtaggta aatcgttgca attcattttc tacaaaacct gtcagtccaa
    11161 aacgcatcca tttattgaac tcttcggcaa ttcctaccga cacagtattg gtcaaagttt
    11221 gcaaagctgc cgtatcattg gtttgtgtaa aaggcaaata agtattttta taaaaatctt
    11281 tttcgacatt cgcttcataa tagcgttttc gcgggtcttc atattttatg gtatgcccga
    11341 aatgggtaac cggcacgtat tctttcctca cggaatcggg tgagattctt attatctggt
    11401 caaaacctat agtatatttc tgatgataaa agaaaacatt ttttcgataa gtggaatatc
    11461 catgtatatt gacggggata tcctgcgttt ttacgccaat cggttcataa atataggaag
    11521 agtcgctaat acctccattt tcatgattga acatattatt gaccgcagcc aaaccaaccg
    11581 cgctgtaatg cttgccatcg taactaccaa acagagaacc cgtaaaacgt ttggcagcct
    11641 gattggcgta ttctcccacc gaatagatgt aatccagcgt cgtgccgaaa ttcaatcgct
    11701 tgttggcatt aaaggtaaac aaaaatttaa tttgatcttc ctgacgataa ttgcttccac
    11761 cggtattgta aacaattttt gtgaaaggag tttttgtatt gtaaaatacg gcactttgca
    11821 tgtcggtaat ataaggataa aaagcatccg aaaaaataaa atcgttactt tccggacgac
    11881 tgaaatacag tttcgactga atgggcgacc ccaaatttcc attatacgaa ttggcaatac
    11941 tgaaccgatc gatcggattt tcatcctgaa agttcaaatg cagcgtatct acctgtacag
    12001 aatctgccat ccccaatgga tcggaaactt tccaagtttt aacataacgg atcgatggcg
    12061 gattgattgc ccaaaccgat aatgcagtaa ctataaataa aataaacaaa aaataccttc
    12121 tcacccgtac tgataaaaaa ttgaaaagtc aatttgttcc taactcattt ttagaaagga
    12181 caaaattaag aaaaatatcc cgtaaatata ttttttggca aagtcttatt gcaaaaaacc
    12241 ttacccaagc aatactcaag taaggttttt gttccgaaaa aagcagaaaa agttagttct
    12301 cgttagcctg actgtctact tctttaatca aataaatcaa ggttggaaaa tggtcgctaa
    12361 aaccattcag ccacactcct ccggcaaatg tacgccacgg atttcccttg tagcggcctt
    12421 cctgctgaat cagaaaatca cgattgaaaa cttccgcttt ccaaaatttc aattcggaag
    12481 gtggagcatt cagcaatgga gctgaaataa tgatttgatc aaacaaattc cactggtcgt
    12541 tataggccaa ggaaccaatt cccctatcca aaattttcca gaaaggattg tacagttcgg
    12601 tttctttcac ttccgaagca ttttttttgg ctcccaaaac cttagccaca ctttcgttaa
    12661 aggggtcatc attcaagtcg cccataacaa ttattttggc atttttatcc acccgataaa
    12721 gagaatcgac aatggagcgc gtcaaagcag cagcggcatt acgtttgggg cgcgaacgca
    12781 tttcgcctcc cgaacgcgat ggccagtgat tcacaatgac atgaattttt tctccaaaaa
    12841 gataaccgct taccattagc tgatcacggg tacggaaagt tgtgtcagca gtatgcaatc
    12901 gataagattt gctattggaa acaataaaat atctcggatt atacaaaagt gcaacatcta
    12961 cgccccgacg gtcaggacca tcataatgga caatttcgta agaacgtttt tttaatgacg
    13021 gttggctcac caaatcttct aacactcctc gattttcaat ttcagaaaca ccaatgatgg
    13081 cagctccgtc aggtgttaca tccaacccga tttgagaaat ggcatacgac attttctcga
    13141 gcttcgattt gtatttcatc gaattccaac gatttgggcc attgggtgta aattcataat
    13201 cgttaacatt ttcattgtca atggtatcaa ataagttttc ctggttgtag aaagctacac
    13261 aggcaatttt tacgttgatt tttttctcgt tcgacgccca aagattgagt gatgaaaaca
    13321 gaaaaaagcc aaaaattaaa gcgtaaaaag aaaaatactt aaagaaattg ttcatcttca
    13381 tggcaaaaaa attattgaga atttatcgtt ttattaaaga gggacaaatg tagtaaaaaa
    13441 gaaatacaaa gaaaaaaatt gaaaaataaa tttagaattt tgtcaacaaa tctcacaaat
    13501 tactgtttta attaaaatta aaaatttcat ttacaactaa ttaatcatat aaataaagaa
    13561 taaacagtat tttaaccaaa aaaaattcta cttcgctgtt ttaattcaaa aaaagatgct
    13621 aacttgcacg cccttttcca aaaaacatta aaaacagaaa aaaacaaatt aacacttttt
    13681 ttttgattaa aaatttcatg ttattcgaag tcgttaataa cgaacaactt ttatgcaatc
    13741 cacaaaaaac aaattaaatc acaactgtat gaaaaaacta tttctgatac tttttgtatt
    13801 ctttttgtgc attccacttt tgatggcaga aacttccatc aaaggaaccg taaagtcggc
    13861 cgacacacaa aatccgattc ccggagtaat tgtttcctta atagggcaga acatttcgac
    13921 acaaaccaat gcggaaggtc aattctctct gaggtttatc gaagccggcg atcaggaaat
    13981 tcgtatttcc aaaatcggct attttactca aatcaagtta attcatattg aacccgacaa
    14041 ggaaaatgat ttgggaactt tttatctgcg tgaagatgtt cagcaacaaa caaaacagga
    14101 agttgttttg caattaagtg aaaatcagat agatgaagat gaaggtcgtg ctactcaaaa
    14161 tatatcggcc gctttgtcat ccaaaggtga tgtatatgtt tctcaatcca gcttttcttt
    14221 tagtccgatg cgcttcagaa caagaggata tgatgataat tttgaatcca cctatatcaa
    14281 cggtgtatat tttaacagct tggaaagagg gggattcaac tattcttcgc tgggtgggct
    14341 gaatgatgcc atgcgaaatc aggattacac ctacggtttt gctccaaatt ccttcagtta
    14401 tggcaatctg ggaggaacta ccaatatcaa tacaaaagcc agcaactacg cagccggaac
    14461 aaaagccagt gttgcctaca ccaatcgttc ctataaattg agaggtcagg ctacttatgc
    14521 taccggttta atgcaaaatg gttgggcatt tgctggttca gctgttgttc gatgggcaga
    14581 caaaggaatt gttgacggca ctttttacaa ttcgtgggga tatttctttt ctgccgaaaa
    14641 aattcttaac aaacaacaca gcctgtcttt cgtaactttt ggttctccaa ctcaacgtgc
    14701 ccaacagggc gccgttaccc aggaagtctt tgatttggcc ggttcaattt attacaatcc
    14761 gtattggggt tatcaggaag gaaaaattcg aaattcacgt atcgtaaaaa gtttcgaccc
    14821 aaccgctatc ctttctcatg aatataaaat cagcaatcat cagttgttgc gaaccggatt
    14881 ggcatttcat tacagccttt acagcaactc ggctttgaat ttctacaatg ctcctgatcc
    14941 acgtcccgat tattatcgca atcttccgag ctttgtgagt gatgattcgt taaaaaatgt
    15001 gattgccaat caatggaaat ctgacaccaa tgtttctcaa gtcaattgga gtgctctcta
    15061 tcaagccaat tacaaaaaca atgtaatcaa tcccaacgga actgcacgat atatcgtaga
    15121 acgccgacac aacaacctga tggaaggagc tttgaactca acctataccc agcaagtgaa
    15181 taaacagttg aaacttattg ccggaataga aggtaaaatg tcgaaaggaa tgcattacaa
    15241 aacacttgac gatttattgg gcggtaatca atggattgac atcgatcagt tttccgaacg
    15301 cgatttccct tcaaatcccg atattattca gaatgatctt agaaaccgaa acgctgttat
    15361 aaaggaaggc gacacttttg gttataacta cgatatcaat atggcttatg ccaatggttt
    15421 tattcaaaat gaatggatac ttccacagtt tgatatctat tatgccgcta aactgaccta
    15481 tacgcaattc tatcgctacg gacacatgca aaacggacgt gctcccgaaa attcgtacgg
    15541 aaaaagcaaa acttggtatt ttgtcgatcc ttcatttaaa gccggtttta cttataaaat
    15601 tgacggacgc aatcgtttgt cattcaatgg actgatagaa agcagagcgc cgctcgccag
    15661 ccaagcttat ctttccgaaa gaataaagga tatgttgatt ccttatttgc aaagtcagga
    15721 tattcggtcg gccgatatta actacaattt tacctactcg accattagag gaagaattgg
    15781 cgtgtttcag acaaatttat acaacacctc cgatatgttg ggatattatg atgacgaata
    15841 cagaactttt atcaaccatg tgttggcaaa atcggacaag gtttataagg gagtcgaatt
    15901 gggaacaacg gtaaaaatca acagtaattt ctccgtttct ttggcaggaa cgtacgccga
    15961 ttatcattat gccaacaatg ccatcggcgt aaaaagtcct gaaaacggtg cttttgccga
    16021 tgttgccgaa accgttctta caaaaggttt aaaaattgct gcaggtcctc aagcagccgg
    16081 aaatattacc atcgattatt tccatcctaa aatgtggttt gccgatgtta ccttaaatta
    16141 tttcgacaac aattatttgg attttgcgcc caaccgtttc accgaaaaca acatggcaaa
    16201 atataccaca cccgaaatga tggctgcttt gggcacccag gaaaaactga aaggtggttt
    16261 tctgctggat gcttctgtag gaaagcttat ttatttgaag catcgtcgtt ctttgaactt
    16321 taacttaagc gccagcaaca tactgaacaa taccaaaatg attaccggtg gttatcagca
    16381 agcccgcttg ccgctctcca atggagccat tgaccccacc ggtctgaaca gatttcccaa
    16441 taaatactat tatgcttggg gctttaacgt gttcttcaat ataggataca agttttaatg
    16501 aataaaaatc atatatttat agatatttaa aatatgacga aaatgaagaa ttttaaaata
    16561 ggtggaattt ccatccttct ggcagcaatt tttacatctt gtgccgaaac agattttgat
    16621 gcaattcccg tcaacaagtt gatgggagat tcattgacta ctacttatac cattgaacaa
    16681 ttgaagaata attttttgac tttaaacgat gcttattccg atacagtcaa ttataaaggt
    16741 ggcttatata cagctgatcc cattcaggcc gaatcggaca ttgtaatcag tggttacatc
    16801 acttctactg atgtggatgg aaatgtttac aagtattttg ttgttcagga acccggagct
    16861 aacggtcaag ccattaaagt ttcgatcgat gcttcaggat tatctgcaat ttatccactc
    16921 ggacagaaag tatggattcg atgcaacggt ttgtacatag gacgttatgg agaagccgaa
    16981 caaattggtt cgcgctatgt aaacaaggaa agatttaaag ttaaaaaatc aactggagat
    17041 accatttacc gaatagaacc aggccgaatg ccatttttga ttgccaacga acacattcac
    17101 gcttacggaa tgcctgatcc tacggttatt aaacccgata ccatgaccat tgctcaaata
    17161 aaagcatcaa atcatactaa acttgtcaac aaactggtat gcatcaaaaa tgccttcttc
    17221 acgggcaaaa gtgaaggcga taatcttacc gatagcgaac tgatttttgc tccatccacc
    17281 aacggagcgg gttatccgca attgcgcgac ataaatgatg gtaccggaga aatcagtatc
    17341 gccaccagcg agtatgccaa gtttgcaaat tatccattac ctgcttccac ttatcggggt
    17401 gatattaccg ttatcgtagg ttggtatcgt aattacacta atgcagcagg cgcatggcag
    17461 ttaactctga gaaccatcaa tgaccttgga aaaggctttg aaagttataa ggcaaaaatt
    17521 cagaaataaa tccgacatta aattacgaaa tcaaaatgaa gaaattcaat tcaaaaatag
    17581 tttttacggc tttggctgtg ttatttacgg caagctgtac caatgttgaa gatgtttaca
    17641 ttgaaaaacc aacttcagat gtaatctatt ccgaaacttt tcagaatggt cttggagatt
    17701 ttaaagcggt aaatgtattc ggcgatcagg tttggaccta caacagcagt gggtatgcca
    17761 ttatgacggg atatgccaat tccaccaatt atgccaacga agactggctt atttctccgg
    17821 aaatagattt gaccaacatc gcttcagctt atttttcatt tgattatgtg atgcgctatt
    17881 caaatgatcc ggcaaacgat gctactgtgt ggatattggc cgatcaagca tacagccccg
    17941 ataccttgcc aaatgtttcg caatgggtaa agctcgactt aaaaccgttt gtcgatcccg
    18001 gaagttggac tttcagcaat tccaacgata tcagtctgaa tgattttgtg ggaaagaaaa
    18061 tcagaatagc attcaggtat atttcttccg caacaaaagc cggcacttgg gaaattaaaa
    18121 atttcacggt aaaaaaaggt actgctaaag tagttgaaag caatgatggc agcgaagaaa
    18181 aaccttttac cgtgaaagaa gccattcaaa atcaggctgg agcttccgca tgggttaaag
    18241 ggtatattgt gggatatatt tggtcgggga ctcaaaataa ttatgttttc agcgctgata
    18301 cttgtactca ggccaccaat atcatattgg ccgataccaa cgatttgtca gctttgtacg
    18361 tatcaaaaat tttagctgtt caattgcctg ccggtgttgt gcgcgatagt ctgaacctga
    18421 aagatcacaa ggacaaattt gggaaaaacg taaccttgta tggttcgcta actaaatatt
    18481 ttggaattgg cggactgaaa tctgtttcct actacaagct tgaagatggc accagcggtg
    18541 gtactaaacc cgttaaagcc attttcagcg aaacatttgc aaattcaaaa ggagatttca
    18601 caatagtaga tgttactaaa gtttctgttc tgcctaatat ttgggctcac gatgcttcat
    18661 acaaatgtat gaaagctact gcttatgtca actctaccaa taccaattat gaatctgaaa
    18721 gctggctcat ttctccgacc ataaatcttt cgggagtaac taatccggta atggtttttg
    18781 attatgtaac cagatatttt gcaaatcccg caacagatgc aaccattcaa gtttctgaaa
    18841 attatcaggg aggcgatcca agaagcacaa cgtggacaca aattccttat ccatttacca
    18901 atatgtcgag ctggacattt gtaacaacca atccgataga tttatcggct tttgccaaca
    18961 aaaatattcg gatagcgttc aaatatactt ctaccagtgc gaaagccgga acttgggaaa
    19021 ttaaaaatct ggtgatttat taatacagtt aatataagcg gttaattgtt ttttattttt
    19081 gaaaagctac agcgataact atcctgtagc ttttttggta tgtctggcat gttattaagc
    19141 ttgcaggatg cgagttccgg tatgtgttga gatgatatag gaaacataag cgaatggcaa
    19201 gggtgttgcg ggtggctgca aatctgaaag aagccatcag caaagctgac gttttgacga
    19261 acagaaatct gataggaggc tctaatgaga gggcaaccga gccatggatt gggaacgccc
    19321 gaaattcaac cgtaattcaa gaagagtaaa tcaggcaaga cgcagcggaa gtttaatatc
    19381 ttatatcccg agaggtctta tagggtgcac cagcagtgta agaaatgcaa cgatgtattt
    19441 tagcccatac aagaagtcag cagagaacat agtattctgt tgaaaaacag aagaaggtct
    19501 gaatctttta attta
//
LOCUS       DAOSIB010000037        19387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_3074_length_19387_cov_11.827281, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000037 DAOSIB010000000
VERSION     DAOSIB010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19387
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3074_length_19387_cov_11.827281"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(352..1107)
                     /gene="tpiA"
                     /locus_tag="PLD44_04830"
     CDS             complement(352..1107)
                     /gene="tpiA"
                     /locus_tag="PLD44_04830"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOF98692.1"
                     /translation="MRKNIVAGNWKMNKTLPEGLALATELNKMLSNYQPKCEVIIAPP
                     FIHLASITATIDTKKIGVSAQNCADKESGAYTGEVSAAMVKSTGANYVILGHSERRTY
                     YGETPVLLKEKVNLALKNELTPIFCIGEVLAERESGIYFDVVKSQIEGSLFHLSAEDF
                     SKIILAYEPVWAIGTGKTATPQQAQEMHAFIRQIITEKYGKSIAENTSILYGGSCNPG
                     NAKELFANEDVDGGLIGGASLKAADFKAIIDAF"
     gene            complement(1189..2352)
                     /locus_tag="PLD44_04835"
     CDS             complement(1189..2352)
                     /locus_tag="PLD44_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOF98693.1"
                     /translation="MSSDIYKNKKMAFPAEQKGKKILFVRICSILLGALFVFSGFVKA
                     IDPLGTTYKIEDYLNAFGSPFSQFVFLAFPAAVFLSTWELVMGLNLLLRIYVRFTTWV
                     ATVFLFFMTLLTFYIALKNPVTDCGCFGDAIVLTNWQTFIKNLFFIALSIFLLINRKH
                     LYPLVLPSLEAIFTVSFVIVGIGISVYSYFHLPIMDFRPYKVGVNIPEAMKIPEGAKP
                     DVYETTFIYEKEGKQKEFNLENYPKNDKSWKFVDQKTKLISKGYRPPIHDFTILNNDN
                     EDISDIILSQSDSTFLLIMYDINKTSQEGAKKAERIFQKAQQNGHSFYAITASTDNDI
                     QKFTERTKISFPFYKADPIILKTVIRANPGLVLIKDGTILGKWNWRDFPENFY"
     gene            complement(2380..2946)
                     /locus_tag="PLD44_04840"
     CDS             complement(2380..2946)
                     /locus_tag="PLD44_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HOF98694.1"
                     /translation="MNQTDKQFDQIAAICRNIFIQKLKDYGASWRLLRPTSVTDQLYI
                     KANRIRNIQVKGFSKINEGIRSEFIGIVNYAIIGLIQLEKGFAEKPDLSVEETIALYD
                     KYMQQAKELMMDKNHDYDEAWRHMRIESMVDLILMKIERTKQIEKNDGKTLISEGIDA
                     NYFDMINYAIFALIKMDENDSQQKNEKI"
     gene            complement(3249..3596)
                     /locus_tag="PLD44_04845"
     CDS             complement(3249..3596)
                     /locus_tag="PLD44_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOF98695.1"
                     /translation="MKKNKLSIQENQELIDFAVTARKYKTQLTKKFLNRKKWENPSPF
                     EIYSFIPGTVVDIFVNEGDIVEEETPLMTLEAMKMQNKIEMPFTAKIKKINVKKGEQI
                     PKDFLIFELEPVE"
     gene            complement(3797..5341)
                     /locus_tag="PLD44_04850"
     CDS             complement(3797..5341)
                     /locus_tag="PLD44_04850"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOF98696.1"
                     /translation="MSAKKNIIALRQKKAIIEKGGGEKAIEKQLAMGKMTARDRIRAL
                     LDEGSFHEYDLFVQHEEKNFGMDTKDLPGDGVITGTGSINGNPVCIYAQDFTVMGGSL
                     GIQHARKITKIMDHALKMKMPCIGINDSGGARIQEGIGALAGYGEIFYRNTMASGVIP
                     QISVILGPCAGGAVYSPALTDFVFVVENISKMFITGPNVIETVLGEKISQEDLGGARV
                     HAEITGNAHFYALSEKECLQQIKELISYIPHNNEEKAARVEPKKPKFKKKIENIIPTN
                     PKLPYDVREIIYALSDDSKFFEVHQLWAPNIVVGFARIEGETVGFVANQPMYLAGVLD
                     CDASDKAARFIRFCDAFNIPLITLEDIPGYLPGVDQEHAGVIRHGAKVLYAYSEATVP
                     KITVILRKAYGGGYIAMNSRHLGADFTFAWPEAEIAVMGPEGAANIIFKKEIMEAEDP
                     DAMRQLKVKEYIEKFANPFVAAGKGYIDAVIEPSETRELLIHSLSVSRKKFDYRPTRK
                     HGNIPL"
     gene            complement(5507..5797)
                     /locus_tag="PLD44_04855"
     CDS             complement(5507..5797)
                     /locus_tag="PLD44_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOF98697.1"
                     /translation="MRRKNTELLSEIIRQMLKDQHLEKPLYEKRIIDAWTSVLGPQIK
                     KYTGHLEVKNGILYVSINSAVLRHDLFLSREEIKNALNKEVGHEVINAIVFR"
     gene            complement(5820..6764)
                     /locus_tag="PLD44_04860"
     CDS             complement(5820..6764)
                     /locus_tag="PLD44_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOF98698.1"
                     /translation="MYSVATKIRLSIFLLFATNIAFGQQLNKNYLSYIEKYHQLAIKQ
                     QKKHGIPASIILAQGLLESSAGQSQLSQLSNNHFGIKCNSDWNGETISWDDDEKNECF
                     RKYKNVADSYEDHSLFLKNRPRYAFLFQLDKTDYQGWAHGLKKAGYATDPTYAYKLIS
                     LIENYNLNRFDKQIFDNNYAERMEIQINTSIPQTIQKVSDYVTHKKFKNNGVPYVIAV
                     PCDTYANIAQEFNLSEETLRSYNEVSATTALQPGEKVYIRPKKNKASKNYPVHVVQAG
                     ESMHDIAQFYGIKTEKLYKMNHIPFTEGAQVGQVLKLR"
     gene            7038..7520
                     /locus_tag="PLD44_04865"
     CDS             7038..7520
                     /locus_tag="PLD44_04865"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOF98699.1"
                     /translation="MFKKSTKVTYSVCRFDELTSLQQQLIDKAKEQVQKAYAPYSGFY
                     VGAALELENGEVIAASNQENSAYPSGLCAERVAVFYANAQYPDIPVKTLAIAAFTNGE
                     FLKMPVTPCGACRQVLLETELRFEKNITILLYGSQKIYIISCVKDLLPLSFDKLQLQK
                     "
     gene            7656..8039
                     /locus_tag="PLD44_04870"
     CDS             7656..8039
                     /locus_tag="PLD44_04870"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HOF98700.1"
                     /translation="MQKSIFTLEYPLKSASANFLWTKIGSPLGLSEWFANEVDLEDSK
                     YVFKWEEFQQSARLLELKPNKSIRFQWLDDLDTPAYFEFRIDTQELTGEVTLVITDFA
                     WEDEKEDAILLWNYQVDELKRIAGM"
     gene            8206..9693
                     /locus_tag="PLD44_04875"
     CDS             8206..9693
                     /locus_tag="PLD44_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOF98701.1"
                     /translation="MFKIKKIDIYVFKNFISLFIVTFLICIFILLMQFLWRHFEDLVG
                     KGIGWSVLFEFFFYSALSLVPMALPLAILLASLMTFGNLGETLELTAMKAAGVSLFQI
                     MRPLLFFISLICVAAFFFSNFALPMAQTKLWTLIFSLKQKSPELEIPVGEFYTGINGY
                     NIYVREKDGKLLKDLMIYDFSAGFDNAIVMVADSGEIQLSSDKKNLLLSLYSGESFEN
                     LKNQQIGSSPSSIPYRRESFRTKEIVVDFNAEFNRFDESLLNDQHISKNISQLSQSID
                     SVNKIVRSMEIQQSKEIMQHPYINWSINQPESTLLPEEKLSTYSFDSLFLSLNEESMF
                     RAIDLAISEVQQIKSSIDFNKIMLEQQIMYVRRHWIEWHRKFTLSFACLIFFFIGAPL
                     GAIIRKGGFGMPVVISVVMFIIYYIIDNTGYKMAREGIWSTFQGMWLSSAVLLPIGIF
                     LTYKAVTDSALFNSENYTRIWNSLVQKIKIWIDKFFALQLKKRHA"
     gene            9704..10948
                     /locus_tag="PLD44_04880"
     CDS             9704..10948
                     /locus_tag="PLD44_04880"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOF98702.1"
                     /translation="MMEIKLNTIEEAINDIRNGKFVIVVDDEDRENEGDFICAAEKVT
                     PEMVNFMITYGRGVVCAPLTEERCEELELEMQVENNTSMHSTPFTVSVDKLEGCTTGV
                     SAADRAATLRALADPSSTPETFGRPGHIFPLRAKSKGVLRRAGHTEAAVDLARLAGLY
                     PAGALVEIMNEDGTMARLPQLMEIAERFDLKIITIKDLIAYRLKFESLIEKGEMVNLP
                     TAYGNFKLIPFRQISNDKEHVALIKGEWEKDEPILVRVHSSCVTGDIFGSLRCECGEQ
                     LHKAMEMIEKEGKGVIVYMNQEGRGIGLMNKIKAYKLQEQGMDTVEANLHLGFDADER
                     DYGVGAQILKDLGVTKIRLMTNNPVKRIGLEAYGLSIVENVPIEIKPNPYNAFYMQTK
                     KERMGHTLRYVNGDKKIDTDIL"
     gene            10978..11754
                     /locus_tag="PLD44_04885"
     CDS             10978..11754
                     /locus_tag="PLD44_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOF98703.1"
                     /translation="MTTSQNNKIAFVSGATSGFGRAIAFRLAKLGYRLIITGRRNERL
                     HRISQQLKEEYSTDVFSLCFDIRDKNACEQAIQSLPADFQNIDLLVNNAGLAAGASSF
                     EESDIADFEQMVDTNIKGLLYITKLIVPKMVERKSGHIINISSIAGIEVYPGGSVYCA
                     TKHAVNAITKGLRIDLVKYGIKVSSISPGMAETEFSLVRYHGDAEKAKAVYAGLVPLN
                     AEDIADAVEFIVTRPKHVSINDIQINPAQQANTYISFRNT"
     gene            11926..13773
                     /locus_tag="PLD44_04890"
     CDS             11926..13773
                     /locus_tag="PLD44_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOF98704.1"
                     /translation="MDSINKTDFSELITDKASQNVSAELSVGENNSAVEDQNTESTPS
                     VVSNYSTLSNEELIEALKKCLIQEVEEVKNDVESIKQAFYRNHRAEVENQKKLFLENG
                     GVEEDFVPKPNELEETFKSLLKEFRDKKAEHLAKVEKEKQDNLLKKQQILEKMKILAD
                     AKDDVSTHINEFRALQQEWKSIGQVPPSSLSELFKQYNAYQEAFWDLIKINNELREYD
                     FKKNLEAKIQICEAAEKLMDEPDVVTAFQHLQKLHNEWHEIGPVSRELREEIWQRFKN
                     ASTAINKKHQTYFESIRKIEEDNLAAKTALCEKIESIDFSVLKTFSEWEEATKNVLAL
                     QDEWRTIGFAPRKLNQKVFERYRHACDAFFSAKAEFFKQVRNSYAENYRKKVELCENA
                     ESLKDNTDWKETSEKFIQLQKEWKAIGPVPRKYSDELWKRFISACDYFFDQKKKNTNS
                     LHGEELENLEKKKNLIARINSLKKSDHPNETVATLKALIEEWSTIGHVPFKEKDKVYK
                     DFRAAVDKKYEELNIDWVSRRLETFKNNLKDMNTKGQRKLIHERDKLLHARERLNSEI
                     ATYENNLGYFTSTTQKGSKVILELERKIDTLKEESRLIEEKIRLIEDNL"
     gene            13913..14698
                     /locus_tag="PLD44_04895"
     CDS             13913..14698
                     /locus_tag="PLD44_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOF98705.1"
                     /translation="MRNFSFLFFTLLIVLFTGCTSQKKIAYFYGVDEKSVANINQSFT
                     KGHEARICTGDMLSITVSGLDPEAVAPFNLPLVSYANPGSTQIYASPVLQSYLVDING
                     YINFPIIGPIKLVGLTKSEAIAYISELLSPYLKNAIITIQFLNYKVTVLGEVLRPGQY
                     SINNERVTVLDALGLAGDMTVYGKRENVLLIRENFGKLEFVRLNLNSEDVFTSPYYYL
                     QQNDILYVEPNVVRSISAQNIPLYLSTISTFATLVTVIYSVTK"
     gene            14736..17123
                     /locus_tag="PLD44_04900"
     CDS             14736..17123
                     /locus_tag="PLD44_04900"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOF98706.1"
                     /translation="MENNIDSRVAANENEVIDLRQLLIKYLSKWHWFVISIVFCFIVA
                     FLYLKITHNKYQVQTTILLRNQNSTPELSNMTMFSNMGLLGVSKEVEDEIQVLSSKTL
                     VRQVIDTLNLGTEYYVKNGLRYDEVYPISPVQIFVSNNFNDTLKQKIILILSPTSKGY
                     DISFQYGEIIEKKYRVSDLSTPLSTPIGSIRFFVNGPLKSGQKIKIISYPLAALTNQL
                     AASISVATVNKNSNAISISTVSPTPHKAKVMLDKLVELYNLDAIFDKNMILTNTADFI
                     ESRLRLISSELLDVELNVENYKKTHGLTDISSEAQLYLETASEYEKKLADIETQLNLV
                     NYVESYINESKNQFNLIPANLGIKDPALQKLIEEYNVALLERMKILRSSNEQNPVITQ
                     MEQQINAIRSNIVKSIAGVKDGLVISKKDLMAYNNEFASRIKSVPTQERQFMEIKRQQ
                     QIKESLYVFLLQKREENALNLASTIPSARTIDAAYTTGSPVSPKRMIIFLLALILGLG
                     LPILVMYVMEFFNNTVQDRKEFQKFVKAPFLGTIGLSKETENIVVKEGRTTPIVEMFR
                     LVRSNLQFMFSDIKSPVILVTSTISGEGKTFIAANLAMSFALMKKKVILVGVDIRNPM
                     LAEYFNLKKNNKGLTIFLADPNCQMDEIIQPSEVHPYLDVILAGAVPPNPAELLMSQR
                     LDELIRELKSRYDYIILDSAPIGIVSDTFQLNRLVDNTICVARLKYTPKEALHFINHT
                     YESKKLKNMATVLNGTNDVSHYGYGYKNEKKYKYRYRETTQNALEKRLNKLKKNK"
     gene            complement(17147..18085)
                     /gene="queG"
                     /locus_tag="PLD44_04905"
     CDS             complement(17147..18085)
                     /gene="queG"
                     /locus_tag="PLD44_04905"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOF98707.1"
                     /translation="MRNFIINTALSIGFDACGITKAETLSADASYLQSWLKAGCHASM
                     KYLERNFDKRVNPKLLVPGCESIIVVLMNYFPSEMQLSAAPQIAKYAYSKIDYHKVLK
                     TKLQQLEKAITEKFGKNSFNDEVQHIFVDSAPVLERRWAEKAGLGWIGKNKQLIHPGL
                     GSYVFIGILMMNAETEYDLPIKNRCGNCRKCIDSCPTQALSEEWLDANKCISYLTIEC
                     KTNIPEEFQGKLSNYAVGCDICADVCPWNRKWAQPNRHPELETASPIKKWNCKQWENL
                     NQEEFESAFSKSAIEYVGYSKLKENISILMEKFKKD"
     gene            complement(18161..>19387)
                     /locus_tag="PLD44_04910"
     CDS             complement(18161..>19387)
                     /locus_tag="PLD44_04910"
                     /inference="COORDINATES: protein motif:HMM:NF025273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HOF98708.1"
                     /translation="PHVLHTQTRMNILFRHVGSIRPLKINYILMKNYFYSLVIIILLI
                     GCQNKNSFTVEGNISSAKGEKLYIEHMGLLKTTSVDSVKLGKNGKFHFTIKRPEYPDF
                     YRLRLNDKLIIFAVDSTEKITIKASKEDLPYNYTLTGSEQSLKIQQLRKSVANIQNKI
                     NKFSSNLAPEEQNQLMKEIEKDIENHKREAQKLILENPRSTAAYYALYQKVNDFYLFS
                     PYVKEDRSYCAAVATAYHTFMPNYTRSQNLYATVMDAIKNERKAESQLRLEELFRTNS
                     TGYINIVLPDKNGIQQKLSDLEGKIVLIDFSIYTARENVEHTFSLRELYNKYHERGFE
                     IFQVSLDENRFLWEKSVQNIPWICVRDERGPNTPYVLSYNISTIPASFLMDRQGNIIA
                     RDLDFKQLDKQIAKLL"
BASE COUNT         5956 a         3309 c         3682 g         6440 t
ORIGIN      
        1 atttataagc atatttttct attggtgtcc attagaaaat actgactaaa aaaaaataat
       61 cctaatatct tattgttcta atggacaatt tgggtttata accattcaca ttttcaatat
      121 aaggaaccca catgtattgc atacacaaac acgaatgaat ataatattca gacatgtggg
      181 ttccatcaga cctttaaaaa taaattatat tctgatgaag aattgaaggt ttattgaatt
      241 ggactaaaac ctgctaacgt agagcctaaa taccactcag tggaatttta ccgaattcca
      301 ttttttagtg tgacatgatc atatttggta agtttcgtta atgttcagcg cttaaaatgc
      361 atcaattatg gctttgaaat cggcagcttt caaagaagct ccaccaatca gtccgccgtc
      421 cacatcttcg ttagcaaaca attctttagc attgcccgga ttgcagctgc caccgtaaag
      481 aatggaagtg ttttcggcta tgctttttcc atatttttcg gtgatgattt ggcgtatgaa
      541 agcatgcatt tcctgtgcct gctgaggtgt ggcagttttt ccggttccaa ttgcccaaac
      601 gggttcgtaa gcaaggatta tttttgaaaa atcttctgca gaaaggtgga acaatgagcc
      661 ttcaatttga gatttcacta cgtcaaaata aattcccgat tctctttcgg ccagtacttc
      721 accaatgcaa aaaatgggtg taagttcgtt tttcaatgcc agattcactt tttctttcag
      781 taggacggga gtttcaccat aatatgttct gcgttcggaa tgacctaaaa taacatagtt
      841 ggcaccggta gattttacca ttgctgcact cacttctccc gtataggctc ccgattcttt
      901 atcggcacaa ttttgtgcag aaacgccaat tttttttgta tcaatagttg ccgttatgct
      961 tgccaaatgg ataaacggcg gagcaataat tacttcacac ttgggctggt aattcgaaag
     1021 cattttgttc agttctgttg ccaatgccaa accttccggt aaggttttat tcattttcca
     1081 atttccggct acaatgtttt ttctcatatt tactgttttt ttattgagtt tgataaattt
     1141 agtttatttg tagaagtttg tctgaagaaa tttattcttt ttcgatgctt aataaaagtt
     1201 ttctggaaaa tctcgccaat tccattttcc caaaatagtt ccatctttta tcagtaccaa
     1261 acccggattg gcgcgaatga cggttttcag tatgatagga tcggctttgt agaaaggaaa
     1321 tgagattttt gtacgttccg taaatttttg aatgtcgtta tcggttgaag ctgtaatggc
     1381 ataaaacgag tgcccgtttt gctgagcttt ttgaaaaatt ctttctgctt tttttgctcc
     1441 ttcctgagaa gttttgttga tgtcgtacat gatgagcaga aaagttgaat cggattgaga
     1501 aagaataatg tcagaaatat cttcattgtc attattgaga atagtaaaat cgtgaatcgg
     1561 aggtcgatag cctttcgaaa tcagtttggt tttttggtcg acaaatttcc agcttttgtc
     1621 gttttttggg tagttttcca gattgaattc cttttgtttc ccctcttttt cgtagatgaa
     1681 tgtagtttca tatacgtcgg gtttggctcc ttccggaatt ttcatggctt ccggaatatt
     1741 tacacccact ttgtagggac gaaaatccat gataggcaag tgaaaataag aataaaccga
     1801 aatgccaatg cctactataa cgaatgaaac agtgaaaatg gcttctaatg aaggaagaac
     1861 aagcggataa agatgtttac ggttgataag cagaaaaatc gacagagcaa tgaaaaaaag
     1921 atttttaata aaagtttgcc agttcgtaag gacaattgca tcgccaaaac aaccacagtc
     1981 cgtcacggga ttttttaaag cgatgtaaaa agtcagcaag gtcataaaaa acaggaatac
     2041 agtggctacc catgtggtga atcgtacata aattcgtaac aaaagattca gtcccatcac
     2101 taactcccaa gtagaaagaa aaacagcagc cggaaaagcc aaaaaaacaa attgggaaaa
     2161 aggactgcca aaagcattca aataatcttc aattttataa gtagtgccca gcggatcgat
     2221 ggcttttacg aaacctgaaa atacaaaaag cgctcccaaa aggatgctgc atattcgaac
     2281 aaatagaatt tttttcccct tttgttcggc aggaaaagcc atttttttgt ttttataaat
     2341 atctgacgac atgagattta attaattgct tcagtggctt taaatttttt cgtttttttg
     2401 ttgactgtcg ttttcatcca ttttgattag ggcgaaaatg gcataattga tcatatcaaa
     2461 ataattggca tcaatgcctt ccgaaatcaa agtttttcca tcattttttt cgatttgttt
     2521 ggttctttca attttcatca atatcaaatc taccatcgat tcgattcgca tgtgtctcca
     2581 tgcttcgtcg taatcatgat ttttgtccat cattagttct ttagcctgtt gcatgtattt
     2641 gtcatacaga gcaatcgttt cttcgacgga aaggtcaggt ttttcggcaa atcctttttc
     2701 caattgaatc aaaccgatga ttgcgtaatt tacaatgccg ataaattcgg atcgaatgcc
     2761 ttcattgatt ttggaaaaac cttttacctg aatgttcctg atccggttgg ctttgatata
     2821 gagctgatcg gtaaccgaag ttggacgcaa caaacgccat gaagcgccat aatctttcag
     2881 cttctgaatg aaaatgttgc ggcagatggc agcaatctgg tcaaattgtt tatcggtctg
     2941 attcataggt aaaagttggt ttttcagtta atgaaaattt tgggttttcc caatcaacag
     3001 ttggataaga aaatacttcc gttgagagta caaaagtata atattttatg aagataggca
     3061 atttgaagtt ttgatgaaaa gcaaactttt gggcaagcga gggctgaaag ttgttttttt
     3121 aattgttatt caattgtttc taccacaata tgtaaaaaaa tctctccgct tttttcgaca
     3181 ttgttgtcgt tattgacgaa gagatttttt tcggtttaaa cacaggatgt tttttcctga
     3241 aattgccgtt attcgaccgg ttccaattcg aatatcagaa agtctttagg tatttgctca
     3301 cctttcttca cattaatttt ttttattttt gcagtgaaag gcatttctat tttattttgc
     3361 attttcatgg cttccagcgt cattaagggt gtttcttctt ccacaatatc gccttcgtta
     3421 acgaaaatgt ctaccactgt tcccggaata aatgaataga tttcaaaagg actcggattt
     3481 tcccattttt ttctgttcaa gaattttttg gttagttgag ttttatattt tcgcgcagta
     3541 actgcaaaat ctattaattc ttggttttcc tgtatagata atttattttt tttcataaat
     3601 actatgtttt taagttgagc aaaaagctcg ttaatcagct ttttggcata agtcggcttt
     3661 tttcaaataa ttttttggtt ctttttactg aaagattatc aagacaaacg gttgtttttt
     3721 atcataattt tgataattaa ttagttgcag aaacaaaaaa taaagatgaa aaatactgat
     3781 ttttcgccga cccaaattat aaaggaatat ttccgtgttt tcttgtcggg cgataatcaa
     3841 attttttcct tgatacactt agcgaatgta taagcagttc acgagtttcc gaaggttcga
     3901 taacggcatc aatataacct ttaccggcgg caacaaatgg atttgcaaat ttttcgatgt
     3961 attccttaac ttttaattgg cgcatggcat cgggatcttc agcctccatg atttcttttt
     4021 tgaaaataat gttggcagca ccttccggtc ccataactgc aatttcggct tcaggccaag
     4081 caaaagtaaa atcggcgccc aaatgtctgg aattcatggc aatgtatcct cctccataag
     4141 cttttctcaa aatgacggtg atttttggaa cagttgcttc cgaataagca taaagcactt
     4201 ttgctccatg acgaataacg cctgcatgtt cctgatctac acccggcaaa tagcccggaa
     4261 tatcttccaa agtaatgagc ggtatgttaa aagcatcgca gaatcgaata aaacgggcag
     4321 ctttatctga agcgtcgcaa tcgagtacac cggccaaata cattggctga ttggcaacaa
     4381 aaccaaccgt ttctccttct atacgagcaa atcccactac aatattgggt gcccaaagtt
     4441 gatgtacttc aaaaaattta gaatcatctg aaagcgcgta aattatttct cgtacatcgt
     4501 atggtaattt tggatttgtc ggaatgatat tttcaatttt tttcttgaat tttggctttt
     4561 tgggttcaac tctggcagct ttttcttcgt tgttgtgagg aatgtaagaa ataagttcct
     4621 tgatttgttg cagacattcc ttttcactca gtgcgtaaaa gtgagcatta cctgtgattt
     4681 cggcatgtac tctggcgcct cccaaatctt cctgtgaaat tttttctccc agtacggttt
     4741 caatcacatt aggacccgtg ataaacattt ttgaaatatt ttctacgaca aacacaaaat
     4801 cggtcagtgc cggagaataa actgcaccac cggcgcaagg tccaagaata actgaaattt
     4861 gaggaattac accggaagcc atagtgttgc gataaaaaat ttcgccataa ccggccaatg
     4921 cgccaattcc ttcctgaata cgtgcacctc cagagtcatt gatgccgatg cacggcattt
     4981 tcattttcag cgcatgatcc atgatttttg tgatttttct ggcatgttga attcctaaag
     5041 aaccgcccat aaccgtaaaa tcttgagcat aaatgcaaac aggatttccg ttgatagatc
     5101 cagttcctgt aataactcca tcaccgggca aatcttttgt gtccattccg aagttttttt
     5161 cttcgtgctg cacaaacaaa tcgtattcgt gaaatgagcc ttcgtccaac aaagcgcgga
     5221 tgcggtcgcg ggctgtcatt ttacccattg ccaattgttt ttcaatagct ttttcaccgc
     5281 cgcctttttc tatgattgct ttcttttggc gcaatgcaat aatgtttttc ttagctgaca
     5341 taacaataaa ttattgtgat taaacataaa aatttaccct ttaatgggct ttgaataagt
     5401 gagcaaaagt aacacttttt ttcattgtat gttacttttt tgaagttttc ttatggaagg
     5461 ataaaattat atcgtttata ttcagtatgt tatagagaaa tatttatcaa cggaaaacga
     5521 tagcgttgat aacttcatgc ccaacttctt tgtttaaagc gttttttatt tcttctcttg
     5581 agaggaataa atcgtgtcgc aaaacggcag agttgatgct gacatataaa attccatttt
     5641 tgacttccaa atggccggta tattttttta tttgagggcc caaaacagaa gtccatgcgt
     5701 caataattct tttttcatac aacggttttt ccaaatgttg gtctttcaac atttgccgaa
     5761 tgatttcgct taataattcc gtgttttttc ttcgcatgat tttaaataaa gagcattttt
     5821 tagcgtaatt tcagtacttg tcctacttgt gctccttcgg taaatggaat atggttcatt
     5881 ttataaagtt tttcggtttt tataccgtaa aattgtgcaa tgtcgtgcat ggattctccg
     5941 gcttgtacca catgaaccgg ataatttttg gaagctttgt tctttttagg ccgaatatat
     6001 actttttctc ccggttgtaa tgctgtggtt gcgctgactt cattgtaact tctgagggtt
     6061 tcttcgctta aattgaattc ttgagcaata ttggcataag tatcgcacgg aacagctatt
     6121 acataaggga ctccattgtt tttaaacttt ttgtgggtta cgtaatcgct cactttttga
     6181 atggtttgtg gaatggaagt gttgatttga atttccattc tttcggcata attgttatca
     6241 aaaatttgtt tgtcgaatct attcaggttg tagttctcaa tcagggaaat taatttgtag
     6301 gcataagtag gatcagtagc ataaccggct tttttcagtc catgagccca accttgataa
     6361 tcggttttgt ctagttggaa aaggaaagca tagcgaggac gattttttag aaacaatgag
     6421 tgatcttcat acgaatcggc aacattttta tattttctga aacattcatt tttttcgtca
     6481 tcgtcccatg aaatggtttc tccattccaa tccgaattgc atttgatccc gaaatggttg
     6541 ttcgaaagtt gtgatagttg gctttgtccg gccgacgatt ccagcaaacc ctgagccagg
     6601 ataatgctgg cgggaatgcc gtgttttttt tgttgtttga tagccaactg atggtatttt
     6661 tcgatgtagg acagataatt tttgttgagt tgctggccaa aagcaatgtt tgtagcaaaa
     6721 agcaaaaaaa tggagagacg aattttagtt gcaactgaat acatgaatgc tattttctga
     6781 aatgattttt aaaaaaaaat tagttggtca atctctgtca aactaaatat gctccacagg
     6841 aatgttgttt gaaaaacata ttagaaaaat tcccgtagtt ttcagcttga tagattaaac
     6901 cggctaataa agtttagttt aattgcaaaa atataattta ttttctcaaa aatgagcgtt
     6961 ttgttgatta tttttttact tttgtacccc ctgctaaagg aaaatttttt gaaagtatca
     7021 ttaaatcaga ttctactatg tttaaaaaat caacaaaagt tacttattcg gtctgtcgtt
     7081 ttgatgaatt gacttcattg cagcaacaat tgatagataa agccaaagaa caggtacaaa
     7141 aagcttatgc accttattcc ggattttatg ttggtgcagc tcttgagctc gaaaacggtg
     7201 aagttattgc ggccagcaat caggaaaatt cggcttatcc ttcgggttta tgtgccgaaa
     7261 gagtagctgt attttatgcc aatgcccaat atcccgacat acctgtgaaa acgcttgcta
     7321 ttgcggcttt tacaaacgga gaatttttaa aaatgcccgt tacgccttgc ggagcatgtc
     7381 ggcaggtttt gctcgaaaca gaattgcgtt ttgaaaaaaa tattaccatt cttctttatg
     7441 gaagtcagaa aatttatatt atctcttgcg tgaaagattt gttgcctttg agtttcgaca
     7501 agttgcagct acaaaaatga gtaccacaat ttttgctgaa ttagtgagtg aaaaattttg
     7561 aatgctcgtt ttaattcatt atattgcagt cgttaatgta ataatgtttt ttaggaattt
     7621 gagagttaga ataagaacga aaaaataaga gaaaaatgca aaaatcgatt tttacattgg
     7681 aatatcctct caagtcggct tccgcaaact ttttgtggac taaaattggt tcgccgcttg
     7741 gcttatcgga atggtttgcc aatgaagttg atttggaaga ttcaaaatac gtttttaaat
     7801 gggaagaatt tcagcaaagt gcacgacttt tggaactgaa gcccaataaa agtatccgtt
     7861 ttcaatggct cgacgatttg gatactcctg cttattttga atttagaatc gatacgcaag
     7921 agctcaccgg tgaagttaca ttggttatta ccgattttgc atgggaagac gaaaaggaag
     7981 acgctatcct gttgtggaac tatcaagttg acgaattgaa acgtattgcc ggtatgtaaa
     8041 agctgctgtt gcttgttctt caaaaattag ccatttgttt gtccgacaag tggttttttt
     8101 atgctttttt ttgctgataa gtttcatttt ttaagcatat ttcatgctac gaataatgta
     8161 aaatcaaact ttttagcgta attttgtaat tttaagaaaa cgaatatgtt taagataaaa
     8221 aagattgata tatacgtatt taagaacttt atttcgcttt tcatcgttac ttttctcatc
     8281 tgcattttta ttcttttaat gcagtttttg tggcgtcatt ttgaagattt ggtcggaaaa
     8341 ggaatagggt ggtcggtatt atttgaattt tttttctact ccgctctttc tcttgttcct
     8401 atggccttac cacttgctat tttgttagct tcccttatga cttttggtaa tctgggagaa
     8461 actttagaac ttacagccat gaaagcagca ggcgtatctc tttttcaaat tatgcgtccg
     8521 ttgttatttt ttatctcgtt aatttgtgtg gccgctttct ttttctccaa ttttgcttta
     8581 ccaatggctc aaacaaagct ttggacattg attttttcac tgaaacaaaa atctcctgag
     8641 ttggaaattc ctgttggcga attttataca ggcatcaatg gttataatat ttatgttcga
     8701 gaaaaagatg ggaaattatt gaaagatttg atgatttatg atttttctgc aggtttcgat
     8761 aatgccatag taatggtggc agattcgggt gagatacagc tgtcatcaga caaaaaaaac
     8821 ttattgcttt ccctttacag tggagaaagt tttgaaaatc ttaaaaatca gcaaattggc
     8881 agttcgcctt ccagcatccc ttaccgacgt gaatcttttc gaacaaaaga aatagtggtt
     8941 gattttaatg ccgaattcaa tcgttttgat gaatctttgt tgaatgacca gcatattagc
     9001 aaaaatattt cacagctttc tcaatctatt gattctgtca ataagattgt gcggtcaatg
     9061 gaaattcaac aatcaaaaga aattatgcag catccctata tcaattggag catcaatcag
     9121 cctgaatcta ctttgctgcc cgaggaaaag ctttcaactt attcttttga ttctcttttt
     9181 ttgtcgctaa atgaggaatc aatgtttcgt gccattgatt tggcaatttc tgaagttcag
     9241 caaatcaaaa gcagtataga ttttaataaa attatgctcg aacagcaaat tatgtatgta
     9301 cgccgacatt ggattgaatg gcatcggaaa tttactcttt cctttgcctg tctgattttc
     9361 ttttttattg gagcaccctt gggagccatt attcgtaaag gaggatttgg catgccggta
     9421 gttatttcgg ttgtcatgtt tattatttat tacattatcg acaataccgg atacaaaatg
     9481 gccagagaag gaatttggtc aacttttcaa gggatgtggc tgagttcggc tgtattgctg
     9541 cccattggca tttttctgac atataaggca gtaacagatt ctgctctttt taattcagaa
     9601 aattacacaa gaatttggaa ttctttggtg caaaaaatca agatatggat agataagttt
     9661 tttgcactgc aattaaaaaa aagacatgct taaattttta taaattatgg aaataaaatt
     9721 gaacactata gaagaagcca ttaatgacat tcgtaatggc aaatttgtta ttgtggtgga
     9781 tgatgaagat cgcgaaaacg aaggagattt tatttgcgct gccgaaaagg ttacgcccga
     9841 aatggtcaat ttcatgatta cttatggtcg tggagtagtt tgtgctcccc tgaccgaaga
     9901 aagatgtgag gaattggaac tcgaaatgca ggtagaaaac aacacttcca tgcattcaac
     9961 accttttaca gtaagcgtag acaaattgga aggatgcact acaggcgttt cggcggctga
    10021 tagagcagct actttacgtg cacttgccga tccttcatca actcccgaaa cttttggccg
    10081 tcccggtcat atttttccac ttcgagcaaa atcaaaaggc gtattgcgca gggctggtca
    10141 caccgaagct gctgttgatc ttgcacgttt agccggtctt tatccggctg gagctttggt
    10201 agaaattatg aacgaagatg gaactatggc tcgtttaccg caattgatgg aaattgcaga
    10261 acgtttcgat ttaaaaatta taaccattaa ggatttgatc gcttatcggt tgaagtttga
    10321 atctttgata gaaaaaggcg aaatggtcaa tttgcctact gcatacggta attttaaact
    10381 gattcctttt cgtcagattt ccaatgataa ggaacatgtt gccttaatca agggagaatg
    10441 ggaaaaagat gagcccattt tggtgcgtgt tcattcctct tgtgtaacgg gagatatttt
    10501 tggttcgctt cgatgtgaat gtggcgagca gttgcacaaa gctatggaaa tgattgaaaa
    10561 ggagggcaag ggtgtgatcg tttacatgaa tcaggaagga cgaggtattg gattgatgaa
    10621 taaaataaag gcttacaagc tgcaggagca gggcatggat acggttgaag ccaatttgca
    10681 tctcggcttt gatgccgatg aacgcgatta cggcgttggt gcgcaaatct tgaaggattt
    10741 gggtgttacc aaaatcagat tgatgaccaa caatcctgtt aaacgcattg gactcgaagc
    10801 ctacggattg tctattgtcg aaaatgtgcc gatagaaatt aaaccaaatc cttacaatgc
    10861 tttttatatg caaacaaaaa aggaaagaat gggacatacg ctgcgttatg taaatggaga
    10921 taaaaaaatc gataccgata ttctttaaaa atacttctat ttttactaaa ttgttttatg
    10981 acaacttctc aaaacaataa aatagccttt gtctcaggtg ccacgtcggg ttttggacgt
    11041 gcaatagctt ttcgattggc aaaattgggt tatcgattga taattaccgg acgacgaaac
    11101 gaaagattgc atcggatttc tcaacaatta aaagaagaat attctaccga tgtattttct
    11161 ctctgtttcg atattcgcga caaaaatgct tgtgaacaag ctatccaatc gttgcctgca
    11221 gattttcaaa acatcgattt gttggttaac aatgccggtt tggcagcagg ggcttcttct
    11281 tttgaagaga gcgatatagc tgactttgaa caaatggtag ataccaacat taaaggtttg
    11341 ttgtatatta ccaaactaat agtgccgaaa atggtggagc ggaaaagtgg ccatattatt
    11401 aatatttctt caattgccgg aatagaagta tatcccggcg gaagtgtata ttgtgcaacc
    11461 aaacatgccg tgaatgccat tactaaaggt ttgcgaattg atttggtaaa atatggaata
    11521 aaagtaagtt ccatttctcc cggtatggcc gaaaccgaat tttcgctggt gcgctatcat
    11581 ggagatgccg aaaaagcaaa agcggtatat gctggtcttg ttccattgaa tgccgaggac
    11641 atagccgatg cggtggaatt cattgtaacc cgtccaaagc atgtttccat taatgacatt
    11701 caaataaatc ctgcacagca ggccaatacc tatatatcat ttagaaatac gtaaaattat
    11761 caataataaa ccgattcgac aaagaaagct taaaatcttt taatgtacat catcgatatt
    11821 taaaataatt attatacttt tgtgaatata aaaatgtttt tgaaaagaaa tagcatcgtt
    11881 ctcgctaaaa aatgggagaa cagatcatga ataacgttac tttaaatgga ttcaatcaac
    11941 aaaaccgact tttctgaatt gattaccgac aaagcatcgc agaatgtttc tgctgagttg
    12001 tctgttggcg aaaataattc agctgtagaa gatcaaaata ccgaatcgac accttctgtt
    12061 gtttcgaatt attcaactct ttcaaatgaa gaattgattg aggctttgaa aaagtgtcta
    12121 atccaggaag ttgaagaggt taaaaatgat gtggaaagta ttaaacaggc attttatcga
    12181 aatcatcgtg cggaagttga aaatcagaaa aaactttttc tcgaaaatgg aggggtagaa
    12241 gaagattttg ttccaaaacc aaatgagttg gaagaaactt ttaaatcttt gttaaaggaa
    12301 tttcgcgaca agaaagctga acatttggca aaggttgaga aagaaaaaca ggacaacctg
    12361 ttgaaaaagc agcagattct ggaaaaaatg aaaattcttg ccgatgcaaa agacgatgtg
    12421 tcgacccata ttaatgaatt cagagcattg cagcaggaat ggaaatctat tggacaggtt
    12481 cctccctcca gtttgtcgga attatttaaa caatacaatg cttatcagga agctttttgg
    12541 gatttaatta aaatcaacaa cgagctgaga gaatatgatt tcaagaaaaa tctggaagct
    12601 aaaatacaga tttgtgaagc ggccgaaaaa ttgatggacg agcccgatgt agttacagca
    12661 tttcaacatt tacaaaagct tcataatgaa tggcatgaaa tcggtcctgt ttcacgtgag
    12721 ttacgagaag aaatctggca acgttttaaa aatgcttcca ctgctatcaa taaaaaacat
    12781 cagacttact ttgaatccat tcgaaagata gaagaagata atttggctgc caaaactgcc
    12841 ctttgcgaaa aaatagaatc cattgatttt tctgtattaa aaactttcag tgaatgggaa
    12901 gaagctacta aaaatgtact ggccttgcag gatgaatgga gaaccattgg ttttgcaccg
    12961 cgaaaactca atcaaaaagt ttttgaacgt taccggcatg cttgcgatgc ctttttttcg
    13021 gctaaagcag aatttttcaa acaggtgaga aacagttatg ccgaaaatta tcgcaaaaaa
    13081 gttgagcttt gtgaaaatgc cgagtcttta aaagataata ccgactggaa agaaacttcc
    13141 gaaaaattta tccaattaca aaaggaatgg aaggccattg gtccggtgcc aagaaaatat
    13201 tccgatgaac tttggaaacg ttttatatct gcttgcgatt attttttcga tcaaaaaaag
    13261 aaaaacacga attctctaca tggagaagaa ttggagaatc tcgagaaaaa gaaaaatctg
    13321 atagccagaa tcaattcatt aaaaaaatcg gatcatccca atgaaacggt tgccacttta
    13381 aaagctctta tagaagaatg gtcaaccatt ggtcatgtac ctttcaagga aaaggataag
    13441 gtttacaaag attttcgtgc agctgtagat aaaaaatatg aagagctcaa tatcgattgg
    13501 gtaagtcgtc gcttggaaac tttcaaaaat aatttgaaag acatgaatac caaagggcag
    13561 agaaaactca ttcacgaaag ggataaattg cttcatgcac gggaaagact taattctgaa
    13621 atagcaacct atgaaaacaa tttgggttat tttacttcta ctacccaaaa aggcagtaaa
    13681 gtgattctcg aattggaacg aaaaattgat accctgaaag aagaaagccg cttaatagaa
    13741 gaaaaaatca ggctgattga agacaattta tagggcgcat ggaaattttt ttaactttaa
    13801 aatgataaaa taatttcatt ttaaagtcgt taattttttt gataaatggt gttataaacc
    13861 taacggttgt aaatttttta ttttgagtat gaatactgaa aaataactga ttatgagaaa
    13921 tttttctttc ctttttttta ctctgttaat tgttttattc acaggatgta cctctcagaa
    13981 aaaaattgct tatttttatg gtgttgatga aaaatcggtc gcaaatatta atcaatcttt
    14041 caccaaaggg catgaagcgc gtatttgtac gggtgatatg ttgtcaataa cagtttcggg
    14101 cttagaccca gaagctgttg ctcctttcaa cttgcctctg gtttcttatg ccaatcctgg
    14161 atctacacaa atttacgctt ctcctgtttt gcaatcttat ttggtagata ttaacggcta
    14221 tatcaatttt ccgattattg gaccaataaa attggttgga ttgaccaaat ccgaagcaat
    14281 tgcttatatt agtgagttat tgagtcctta tttgaaaaat gccattatca ccatacaatt
    14341 tttgaattat aaagttacgg ttctgggaga agtacttcgt cccggacaat attccatcaa
    14401 caacgaaaga gttacagtgt tggatgcttt gggtttggct ggcgacatga cagtttacgg
    14461 aaagagagaa aatgtgctgt tgatacgtga aaactttgga aaattggaat ttgttcgttt
    14521 aaatttgaat tccgaggatg tgtttacctc tccttattat tatttacaac aaaacgatat
    14581 tttatatgta gaacccaacg ttgtaagaag catttcggct caaaatattc cgttgtatct
    14641 atctactatc tctacgtttg ctactttggt tacggttatt tatagtgtta caaaataatt
    14701 tttagtttaa attgtgttat ttttatctgt tataaatgga aaacaatatt gattctcgcg
    14761 ttgctgcaaa cgaaaatgaa gtgattgatt taaggcaatt actaataaaa tatctgagta
    14821 aatggcattg gtttgttatt tcaattgttt tttgttttat cgtagccttc ctgtatctga
    14881 aaattaccca caacaaatat caggttcaga ccaccatctt gcttcgtaat caaaattcga
    14941 ctcctgaatt gtccaacatg accatgtttt ctaatatggg gttgttggga gtttcgaaag
    15001 aggtggaaga tgaaattcag gtactttctt ctaaaactct tgttaggcaa gtaattgaca
    15061 ccttgaatct aggaacagaa tactatgtaa aaaatggatt aagatatgat gaagtttatc
    15121 ccatcagtcc ggttcagatt ttcgtgtcaa ataatttcaa tgacacttta aagcaaaaga
    15181 taatcttaat attgtctcct acttcaaaag gttacgatat ttcatttcaa tatggagaaa
    15241 ttattgaaaa aaaatatcga gtatccgatc tttccacacc actttctaca cctattggtt
    15301 caattcgatt ttttgtaaat ggtcccttga aaagtggaca aaagataaaa attatttcct
    15361 atccacttgc tgccttaacc aatcagctcg cagcgtccat ttctgtggct acagtgaaca
    15421 agaattctaa tgccatcagc attagtactg tttcgcctac acctcacaag gcaaaagtca
    15481 tgctcgataa attggttgag ctttataatt tggatgccat ttttgataaa aatatgatat
    15541 tgaccaatac tgccgatttt attgagagtc gtttaaggct tattagcagc gaattgctcg
    15601 atgtagaatt aaatgtagaa aactataaaa agactcacgg attaactgat atttcatctg
    15661 aagcccaact ttatctcgaa acggctagtg aatatgaaaa aaaacttgcc gatatagaaa
    15721 cacagctcaa tttggtaaat tatgttgaaa gttatatcaa cgagagtaaa aaccagttca
    15781 atctgattcc tgcgaacttg ggtattaaag atccagcctt gcaaaaactt attgaagaat
    15841 acaatgttgc cttattggaa cgaatgaaaa ttttgagaag ctcgaacgaa caaaaccccg
    15901 ttattaccca aatggagcaa caaatcaatg ccattcgcag taatattgta aaaagtattg
    15961 ccggtgtaaa agatggattg gttatttcta agaaagattt aatggcctac aacaatgaat
    16021 ttgcttcaag aattaagagc gttcctactc aagagcgtca gtttatggaa attaaacgtc
    16081 agcagcagat caaggaatcg ctctatgttt ttttgcttca gaaacgagaa gaaaatgctc
    16141 taaatctggc aagtactatt ccatcagcca gaacaattga tgctgcctat accaccggtt
    16201 cacccgtttc tcccaaaaga atgattattt ttttacttgc gttgattttg ggactcggat
    16261 tgccgatatt ggtgatgtat gtgatggaat tttttaataa tacagttcaa gatagaaaag
    16321 aatttcagaa atttgtcaag gcaccatttc ttggaactat tggattaagt aaagaaacgg
    16381 aaaatatagt tgtgaaagaa ggtagaacaa ctcccatagt tgaaatgttc aggctagtgc
    16441 gttccaattt gcagtttatg ttttccgaca taaaatcacc tgtcatattg gtaacctcaa
    16501 ccatcagcgg agaagggaag acctttattg cagcaaatct tgccatgtcc tttgccttaa
    16561 tgaaaaagaa agtcattttg gtgggcgtag atatcagaaa tcccatgttg gctgaatatt
    16621 ttaatttaaa aaagaacaat aaggggttga caatattttt ggctgatccg aattgtcaaa
    16681 tggatgaaat tattcagcct tccgaggtac atccctatct tgatgttatt ttagcaggag
    16741 cagttcctcc caatcccgct gaactattga tgagccaacg acttgatgaa ttgatcaggg
    16801 aattgaaatc ccgttacgat tatattattc tcgattcggc tcctattgga attgtttccg
    16861 atactttcca actcaatcgt ttggtggaca atactatttg tgtagctcgt cttaaatata
    16921 cgccaaaaga agctttacat tttattaatc atacgtatga atccaaaaag cttaaaaaca
    16981 tggctactgt attgaatgga acaaatgatg tttctcatta tggatatgga tataaaaatg
    17041 aaaagaaata taagtacaga tatagagaga ctactcaaaa tgctttggaa aaaagactca
    17101 acaagctaaa aaagaataag tgataaactg acactttatt aattttttag tcctttttga
    17161 acttttccat caatatggaa atgttttctt tcagcttgct atagccaacg tattcgatgg
    17221 cagatttcga aaacgctgat tcaaattcct cttggttgag attttcccat tgcttgcagt
    17281 tccatttttt tatgggagaa gctgtttcca attcgggatg cctgttgggt tgtgcccatt
    17341 tcctgttcca cggacaaacg tcggcacaaa tatcgcagcc gaccgcgtaa ttggataatt
    17401 tgccctgaaa ttcttcagga atatttgtct tgcattctat agtcagataa gaaatacatt
    17461 tatttgcatc cagccattct tccgatagcg cttgagtggg acaggagtcg atgcattttc
    17521 ggcagtttcc gcagcggttt ttaattggca aatcatattc tgtttcggca ttcatcatta
    17581 aaattccgat aaagacataa gaacccaatc ccggatggat gagttgtttg ttttttccaa
    17641 tccatcccaa accggctttt tctgcccatc gacgttccag tacgggagca gagtccacaa
    17701 aaatatgttg tacttcatcg ttaaaactgt ttttaccaaa cttttctgtg atggcttttt
    17761 ccaactgttg aagttttgtc tttaaaactt tgtggtaatc gattttgctg taagcgtatt
    17821 ttgcaatttg gggcgcggct gacaattgca tttcggatgg aaaatagttc atcagaacta
    17881 caattattga ttcacacccc ggaaccagta attttggatt cactcttttg tcgaaatttc
    17941 tttccagata tttcatgctt gcgtgacagc ctgcttttag ccacgactgc aaataacttg
    18001 catctgcaga aagtgtttcg gctttggtta ttccgcaagc atcgaatccg atagagagtg
    18061 cagtattgat aatgaaattt ctcattcgat tttttgttga gaaagacaac cgaattgagc
    18121 ggtatagatt acgattaaac tttttattgt atcaaataaa ctacaaaagt tttgctattt
    18181 gtttgtccaa ttgtttgaaa tccaaatcac gggcaataat attgccttgc ctgtccatca
    18241 aaaaagatgc cggtatagta gaaatgttgt aagaaagtac atatggcgta ttgggtcctc
    18301 gttcgtcacg aacacaaatc caaggaatat tctgaacgga tttttcccaa agaaagcgat
    18361 tttcatcaag tgaaacttga aaaatttcaa aaccgcgttc gtgatacttg ttgtataact
    18421 cacgcagcga aaaagtatgt tcaacgtttt cacgagctgt ataaattgaa aaatcgatca
    18481 aaacaatttt cccttccaaa tcggacagtt tttgctgtat tccattctta tcgggcaaaa
    18541 caatattaat gtagccggtg ctgtttgttc taaataactc ttccaatcgt agttgacttt
    18601 ctgcttttct ttcatttttt atagcgtcca ttaccgttgc gtaaaggttt tgacttctgg
    18661 tgtagttagg catgaaagtg tgataagcag tggcaacagc cgcgcaataa gatctatcct
    18721 cctttacgta aggtgaaaac aaataaaaat cattcacttt ctgataaagt gcataataag
    18781 cagctgtaga gcgtggattt tccaagatta atttttgagc ttcccgctta tgattttcaa
    18841 tgtctttttc aatttctttc attagctgat tttgctcttc tggagctaag ttgcttgaaa
    18901 atttgttgat cttgttttga atattggcta ccgatttcct caattgttga attttaagcg
    18961 attgttcaga acccgttagt gtgtaattgt atggcaaatc ttctttactt gctttgattg
    19021 taattttttc tgttgagtct acggcaaaaa tgattaattt atcgttcagt cgcaagcggt
    19081 aaaaatcggg atattcaggt cgttttatgg taaaatgaaa ttttccgttt ttaccaagtt
    19141 taactgaatc tacagaagtt gtttttaata accccatgtg ttctatgtat aatttttctc
    19201 ctttggctga agatatgttc ccttcaacag taaaagaatt tttattttga caaccaatca
    19261 gcaaaataat aatgacaagt gaataaaaat aatttttcat cagaatataa tttattttta
    19321 aaggtctgat ggaaccaaca tgtctgaata atatattcat tcttgtttgt gtatgcaaca
    19381 catgtgg
//
LOCUS       DAOSIB010000038        18649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_3206_length_18649_cov_10.991126, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000038 DAOSIB010000000
VERSION     DAOSIB010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18649
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3206_length_18649_cov_10.991126"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1556)
                     /locus_tag="PLD44_04915"
     CDS             complement(81..1556)
                     /locus_tag="PLD44_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOF98709.1"
                     /translation="MKQLFNLLLILLLGVQFLSSQIIKEFPIAVGSEAASALSAAFDG
                     TNYLVPILGDANSEYTLTVQFISKTGELSGNRIALNAYGPVAPMVVFDGTNYLVVWQD
                     VEHHLYARFLSPSGNWQGEAVLIATDVIFDLRPFNVSYSGSNFLVAYVKSDFGLYGQL
                     LDKSGNLIGSSFFINNFGITNPQNSSSKEFNMASDGTNYLIVWFNHGGGSSNSILGQF
                     VGKNGTLVGNAFTIDPPSPDHNPVSLIFDGNKYVISYDICFNRNSPYSCNDHGAYFRF
                     VSTSGTVGEKTTLAETAGVHNAFTFVCSDGNNYLFTWESQSLSDNSADNHIKGRYYNM
                     SGTPLGEEFNIFTVPGSEGKIPLGGVMLFDGNNFLVGVTKAVLGETGFSDGDVYGILV
                     QKYPSGLNNPQNNEKLKLYNYPNPFVNTTTFYFQLPLRSKVTLIIYDALGKQLMTVID
                     ETLAAGEHQIPWQTNKLTGGLYFYKIQTEDFSLTSKFVVQK"
     gene            complement(2468..4903)
                     /gene="lon"
                     /locus_tag="PLD44_04920"
     CDS             complement(2468..4903)
                     /gene="lon"
                     /locus_tag="PLD44_04920"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOF98710.1"
                     /translation="MKHSFDDFNIEELTQPSEAFPIISFEEEKNGDVSLESGDILPIL
                     PLRNMVLYPGVLLPVSVARPKSLKLVREAKESNHYIGVCTQKDKNIDDPTPDQLYSMG
                     TVAKVVRILEMNDNTTTVILEGKSRFFLGEVLTLKPYMKARINLAPEIIPSAEDGVFP
                     ALVATIKDMAAKIIANAGIMPPEAVFAIRNIDNPVFLINYVCVNFGLNVQEKQRLLEI
                     DDILDRGYQLLDLLNKEWQLLEIKISIENKAKEGIDQQQKQYFLQQQMKTIQEELGGA
                     SPEQDILDFREKAKQKKWNENVAALFEKELKKLERMSPHSPDYTTQLNYVETLLELPW
                     NEYTKDNFNLKNARKILEQDHFGMETVKERILEHLAVLKLKGDMKSPIICLYGPPGTG
                     KTSLGKSIARALNRKYVRISLGGLHDEAEIRGHRRTYIGALPGRIMQNIQKAQSANPV
                     FVLDEIDKVAADYKGDPSSALLEVLDPEQNTSFHDNYLDVDFDLSKVMFIATANNLNT
                     IPRPLLDRMELIEVSGYTQEEKVEIARRHLVPKELENHGLTKEQLILTKPVIEHLIEN
                     YTRESGVRELDRQIASLMRKIAKKVASNEPYNVQLTVDDLKTFLGVPRYTKEKYQGND
                     YAGVVTGLAWTQVGGEILYIESSLSKSKSPQLTLTGNLGEVMKESAVIALEYIKAHAE
                     QLGIDSEMFEKWNVHIHVPEGAIPKDGPSAGITMLTALVSAFTKRKVRKNLAMTGEIT
                     LRGKVLPVGGIKEKILAAKRAGITELVLCEENKKDVEEINASYLKGLHFHYVNDIMEV
                     INFALLDEKVRME"
     gene            complement(5337..5819)
                     /locus_tag="PLD44_04925"
     CDS             complement(5337..5819)
                     /locus_tag="PLD44_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOF98711.1"
                     /translation="MRTFFIEDQVEIENIINACDICFVGISELDGSPYVIPMNFAFDH
                     NEVILHSAPEGKHQLLLQKDNRVCITFCSDRKLMYQHPDVACSYSMNSKSVLVKGKVI
                     FIENLEEKEQAMNLFMKKYTQRSDFKYSLPALRNVKVWKVKVESITAKAFGQNFKTQQ
                     "
     gene            6243..6407
                     /locus_tag="PLD44_04930"
     CDS             6243..6407
                     /locus_tag="PLD44_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98712.1"
                     /translation="MKKLFLLCAVVFAFVACGPKENKEAAATEDSVVVVDTVVAPVDT
                     AAVDTTVAPM"
     gene            6582..7433
                     /gene="hisG"
                     /locus_tag="PLD44_04935"
     CDS             6582..7433
                     /gene="hisG"
                     /locus_tag="PLD44_04935"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOF98713.1"
                     /translation="MLRIAVQTKGRLFDDSMSLLNEAGIKIELGKRLLLIPAQNFPLE
                     VLFLRDDDIPESVADGVADIGIVGENEFAEKQKNVKLIRRLGFSKCRLSLAVPKEAEY
                     SDICWFEGKTIATSYPVILSNFLKEKGIRAEIHVITGSVEIAPSIGLADAIFDIVSSG
                     STLVTNHLKEVEVVMKSEALLIANPKLSEAQKKILDELLFRIEAVKMAEDKKYVLMNV
                     PTSQLEKIIEIIPGMKSPTVMPLAKEGWNSVHAVIAEKEFWDIIGKLKACGAEGILVL
                     PIEKMIL"
     gene            7448..8173
                     /locus_tag="PLD44_04940"
     CDS             7448..8173
                     /locus_tag="PLD44_04940"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOF98714.1"
                     /translation="MNLCIDQGNSSIKMGIFDQSKLVKTFVYKNFDRKSVLSMLRNYP
                     VDRCIYSSVINKNASAVAFLKKNIAQVLLLTHETPLPIENKYQTPKTLGKDRLAAVVG
                     AGFLEPNRDVLVIDAGTAITFDFMDAKRNYWGGNIDVGLELRLMALHTFAKKLPLVKP
                     VQEVPLMASNTEMALAAGALNGIIFEIDGYINALKIEYPDLSTFLTGGSLIFFDKKLK
                     NSIFVEKNLVLIGLNYILQYNVQ"
     gene            8211..9413
                     /locus_tag="PLD44_04945"
     CDS             8211..9413
                     /locus_tag="PLD44_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98715.1"
                     /translation="MQDTSSQNNTNSPYTRFGFGEINDNHNSEQRAMGGVALGVRSVS
                     SINSINPASYSVVDSMTFMFDFGVSALRSQFKDPSGKSSNFTANLDYITIQFPLSKNI
                     ALSAGIQPYSFSGYNFYTTEILTIPNNSNTPDSLNVTKSFTGSGGFSEVYMGLSIDFL
                     QHWALGVNAYYMFGTINNYRNISFSSTNYTSTTELNYIEANNFRFRYGMQYFTGFNKK
                     NDLNIGLIFEPKKNFNVKSSKINSGVLTDTTNYDTDFDLPMLLGGGIYYTYNKKLSLG
                     IDYTFQNWANTRFYGKTDSLVNRNKISIGAEYLPNSSGRKYSDHIRYRLGLNVNNPYY
                     KIDGKSLDNNFGITFGIGLPLPYSRTMLNTSFEYGKIGNYSSLREDYFKFTFNVCVNE
                     NWFFKRKL"
     gene            9444..10817
                     /locus_tag="PLD44_04950"
     CDS             9444..10817
                     /locus_tag="PLD44_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98716.1"
                     /translation="MKTKILMLLLAAIFSTNTFAQKGKNVQTSATTENSSEQVTDSMC
                     LIYISLFNEAAKNKQYADAIGPWTLAYNECPGANRVIYTRGREILQWKLSQAKDLTSY
                     KEAFDELMAMYDKRIKYFGDDSRYPTPWILGVKALDYITYVKGDDLKKPAYEWLEQSI
                     NGLKENSELEVLRQFIMLSYNLYKANPEHAEKFIADYLKVNDILETQIANPEYKSNEL
                     ANQIKQGLDVLFAQSGAADCQMLDKMYASKVTQNSTNLEFLNNVLSFYNRIGCIQSDV
                     YFSAALSAHKIQPSTESANALAEMSYKKKDYSGAIKFYEQATQLANNNKEKATYQYKI
                     AQIYYGDLKNYSRAREYALKSLEFNPNNGSAYLMIGIMYANSNIYDDPVLQKSVYWAA
                     VDKFIKAKQVDNDPKIIEEANKLIQTYINYFPSKEDIFFHPQLEEGKSFFVGGWIGES
                     TICRASN"
     gene            10906..11505
                     /gene="lptC"
                     /locus_tag="PLD44_04955"
     CDS             10906..11505
                     /gene="lptC"
                     /locus_tag="PLD44_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOF98717.1"
                     /translation="MFIVVMLFLSFACNRPTKPEIVQIESDPSLIPTLHATDITTVIS
                     DSGITRYRISAPQWDIYDKVPDPYWEFPYGIHLERFDMNLQVDANIHSKYARFFVNKK
                     LWELKNDVKATNLKGELFETEQLFWDQNSQKIYSDSLIKVTQATQIINAIGFESDQSM
                     NYYVFRNVKGIFPVDESKDTVPTSQSSNAGTPIRAMNAK"
     gene            complement(11537..12931)
                     /gene="glmM"
                     /locus_tag="PLD44_04960"
     CDS             complement(11537..12931)
                     /gene="glmM"
                     /locus_tag="PLD44_04960"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOF98718.1"
                     /translation="MALIKSISGIRGTIGGRAGEGLTPLDIVRFSAAYAKFMKQNAGN
                     DSNKIVVGRDARISGDMVYHLVAGTLSGQGLEVVNIGLATTPTTELAVIYEKALGGII
                     LTASHNPMQWNALKLLNEKGEFLNAKEGEEVLKIAAEENFDFASVEQLGKISENFTFL
                     QKHIDNILSLNLVEVDAIKKADFTVAIDAVNSVGGIAVPALLEALGVKKVIKLNCEPT
                     GIFAHNPEPLPEHLSEISSLVKESKADVGFVVDPDVDRLAIICEDGSMFGEEYTLVAV
                     ADYVLQHTPGNTVSNLSSSRALRDITQKRGGKYSAAAVGEVNVVTLMKQTNAVIGGEG
                     NGGVIYPASHYGRDALVGIALFLSYLAKSKKTVSALKNEYPQYFISKNKIELTPSIDV
                     DKILATIKNNYQLQYEVNDIDGVKIDFPTGWVHLRKSNTEPIIRIYAEAKTKEQAEAF
                     ANAIMEEIRRIAAA"
     gene            complement(12962..13510)
                     /locus_tag="PLD44_04965"
     CDS             complement(12962..13510)
                     /locus_tag="PLD44_04965"
                     /inference="COORDINATES: protein motif:HMM:NF027435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4827 family protein"
                     /protein_id="HOF98719.1"
                     /translation="MKKFIFLFLGFLPVILIFSSCNNTKTYAELLKDEKAIIASYIKR
                     SNIQVVTTFPADGEWDNKYVKTSSGLYFHLVSPGDTAANADTVAKNDRVVIRYRQYTL
                     TEYPDTIYYWNTIDYPYPISFTYLDYTQACTAWHEAVSYMKRNNSEAKLIVPSKIGLS
                     EYINSVTPLGYDFKIVGIQKSY"
     gene            complement(14299..17142)
                     /locus_tag="PLD44_04970"
     CDS             complement(14299..17142)
                     /locus_tag="PLD44_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOF98720.1"
                     /translation="MDERDDREEITGAENEEDFVQQADIEDNSETKPSGHSSYKIPKI
                     ENSEVKHHLSGMYQNWFLDYASYVILERAVPDIYDGLKPVQRRILHSMRRLEDGRYNK
                     VANIVGHTMQFHPHGDASIGDALIQLGQKDLLIDCQGNWGNILTGDSAAAPRYIEARL
                     SKFALETVFNPKTTEWKLSYDGRNKEPVNLPVKFPLLLAQGVEGIAVGLNSKILPHNF
                     NELIDACIAYLKGEDFELYPDFQTGGLIDVSHYNDGERGGVVKIRAKINKVDNKTLSI
                     TEIPYGTNTSKLIDSILKANDKGKIKIRKVDDNTASSVEILVHLQPGTSPDKTIDALY
                     AFTDCEISISPNCCVIHDRKPRFMNVSEMLRVSCDHTKDLLEAELNIQKQELLEQLFF
                     TSLEKIFIENRIYKDKGFEDSSSQDAVVNYIDQRLEPFKENFIRAVTREDILRLLDIK
                     MVRITKFDSDKANDTLIAIQKKIDEVDFHLNHLIDYTISWYQGLKDRYGKNYPRRTEI
                     RNFETIIATKVAEANEKLYVNREEGFIGTSLKKDEFVCNCSDIDDIIVFFKDGRYKVT
                     KVAEKMFVGKNILYVNVFKKNDKRTIYNVVYRDGKAGPYYIKRFAVNGITRDKEYDLT
                     QGSAGSKILYFSANPNAEAEVIRVTLKPKPRMTKLVFEKDFGEIAIKGRQAMGNILTK
                     NEIHKITLKQKGGSTLGGRQVWFDHDVLRLNYDKHGEYLGEFQSDDLILIVSKNGEYY
                     TTNFDAANHYENNILIIEKFDENKVWSAVVYDGELKNYYLKRFQLTPSQKKNSFVGEH
                     PESRLILLSSVDFPRFEVVFGGHDAHRNPLIVDAEEFISVKSYKAKGKRLTTFEVETV
                     NELEPVRFKEKNNEESTVINSDEEEDNENASENDRISNSSDAQESPEKATDVERKTNV
                     NSQTKEDAVSNAINEDRPEIHSTDEEGSQLTLF"
     gene            complement(17167..17916)
                     /locus_tag="PLD44_04975"
     CDS             complement(17167..17916)
                     /locus_tag="PLD44_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOF98721.1"
                     /translation="MTSWTQRTELLIGKSGMEALQQASVLVVGLGGVGGLAAEMICRA
                     GIGKMTIVDHDTINPTNLNRQILASVNTISQYKTDVMAQRLLQINPDLKLKTFNQWAE
                     VSNIEELLETEKYDFVVDAIDTVTPKVFLIKSCLERGIDIISSMGSGGRMDPSKVRIT
                     DISNTHTCPLAKAVRQRLSKMGITKGLAVVFSDELPNKNAVLPVEDEKFKKSTVGTIS
                     YMPAIFGCMMASHVIREILRKNHFQKTLDRT"
     gene            complement(18180..>18649)
                     /locus_tag="PLD44_04980"
     CDS             complement(18180..>18649)
                     /locus_tag="PLD44_04980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOF98722.1"
                     /translation="LCKLLPSPFDLIFRRFLPKSWTRKRTEKFEMRASGYDKVMDVPY
                     LSGCFMFFRTDALREVGLFDERFFLYPEDIDLTRRIRRKFRTVFYPHVSVVHYHERGS
                     YKNLRMLWIHIINMIKYFNKWGWFCDAERKRVNRETMKQYFSQCHEIKIKSIN"
BASE COUNT         5937 a         3595 c         3165 g         5952 t
ORIGIN      
        1 aaactttctt ccatattttg ttttataatg agctgaaaaa attcaaaaaa tcaattttat
       61 gccaatgact gaaaaaacct ttatttttga accacaaatt tgcttgtcaa actaaagtct
      121 tctgtctgaa ttttgtaaaa atataaacct ccggtgagtt tgtttgtctg ccagggaatt
      181 tgatgctcac ctgcagccaa cgtttcgtca atcactgtca tcaattgctt tcccagtgca
      241 tcataaatta tcaatgttac ttttgatcta agaggtaatt gaaagtaaaa agttgttgtg
      301 ttgacaaaag gatttggata attataaagc ttgagttttt cattattttg tggattattt
      361 aaaccagatg gatatttttg aaccaaaata ccataaacat ctccatcgct aaatcctgtt
      421 tctccaagaa cagcctttgt aaccccaacc aaaaaattat tgccatcaaa caacattaca
      481 ccaccaagtg gaatttttcc ttcagaaccg ggaacggtaa atatgttaaa ttcttcaccc
      541 aatggcgtac ccgacatatt ataatatctt ccttttatat gattatcggc agaattatcg
      601 gataacgact gactttccca tgtaaacaga taattattcc catccgagca tacaaaagta
      661 aaagcattgt gtacaccagc tgtttccgct aaagtagttt tttctcctac agtaccactg
      721 gtcgaaacaa atcggaaata agctccgtga tcattacaac tataaggact gttgcgatta
      781 aaacaaatat cgtaactgat aacatatttg ttcccatcaa aaatcaaact taccggatta
      841 tgatcaggtg agggaggatc gatagtaaaa gcatttccta ccaaagttcc atttttgccg
      901 acaaattggc ctaatattga gtttgaactg cctccaccat gattgaacca tacaatcaga
      961 tagttcgttc catcagaagc catattgaat tcttttgaac ttgaattttg cggattggta
     1021 atgccaaaat tattgataaa aaaggaactt ccaatcagat ttcccgattt atccaataat
     1081 tgtccgtata atccaaaatc cgattttaca taagcaacca aaaaattaga accggaatag
     1141 ctcacattaa aaggacgtaa atcgaaaatc acgtcagtag caattaaaac tgcttctcct
     1201 tgccagttac cggaagggct cagaaaacga gcataaagat gatgctcaac atcctgccaa
     1261 accacaagat aattggttcc atcgaaaaca accattggag caaccggtcc ataagcattt
     1321 aaagcaattc tatttcctga caactcgccc gttttactga taaattgaac cgtaagcgtg
     1381 tactcactat tggcatctcc cagaattgga actaaataat tggtgccatc gaatgctgcc
     1441 gataaagccg aagcagcttc actgccaact gcaattggga attccttaat aatttgtgaa
     1501 gaaagaaatt gaactcccaa caatagaatc aaaagtaaat tgaataattg tttcatggca
     1561 ttaatttttt tttgttaatt ttatttgaga tgataatcga aacaaattta aaaatcaaac
     1621 caacaaattt taaattcttt tgctaaagaa cttaaaaaag ataagaaact aattaattta
     1681 ttggtaaaag tattatcaaa ttatccgaaa tgtactttta ttaataattt tttgtattaa
     1741 ttatattttt taaaatttaa gcattattga aattgtaaaa aatatttaat taataaatta
     1801 aggaatgaat tgaagagtta catcagtaaa aatctgtaga gagtcgtttt tttaatttat
     1861 tctattatat tcaattcaat atcaataaaa tagttgtaaa taaggttgaa taaatttgtc
     1921 gaggttagca aacactataa aaatgataga aaaattacca ataaagatca atgagattta
     1981 ttttgttctt tgttgaaaaa ctttataata gatatgaacc atgaattaat tcaattatgg
     2041 gacaaaatag actgaagtta ctttcttact ttgagaagga attggtatct atattttgcc
     2101 aatggcccca gagtgtttca aatggagaag ggtagagtgg atgttgctca aacatttgta
     2161 caatttcgag ataaacgata actgaattat tgaatacgga atgtttaatt tcagcatttt
     2221 tgcagaagtg tgttttttga ataccgtttg ccgtttgatc agaacgattt tgtccatttt
     2281 cggcaaacca atcggcgaag acggcataca aattaacgct ttattggcta ctcaagcttg
     2341 gaatttgaaa aaaatgatgt gaaaactcaa agaagagttt tttgtttcat ttcaacaaaa
     2401 atttctaatc ttgccactaa aacaaacttt taaggattga ctaatcatac ttgacaaatc
     2461 tttaaattta ctccatccta actttctcat ccaacagagc gaaattgatg acttccatta
     2521 tatcgtttac gtagtgaaaa tgaagacctt tcaaataaga cgcattaatt tcttccacat
     2581 cttttttgtt ctcttcgcaa agaaccaatt cggttatgcc tgctcttttg gccgcaagta
     2641 ttttttcttt aattccacca accggtaata cttttccacg taaggtgatt tcgccggtca
     2701 tggcaagatt tttccgaact tttcttttgg taaatgccga aacaagcgct gtaagcattg
     2761 taattcccgc cgacggacca tctttgggaa tagctccttc gggaacatga atgtgaacat
     2821 tccatttttc aaacatttca ctatcaattc ctaactgttc ggcgtgtgct tttatgtatt
     2881 ccaatgcaat cacagcagat tctttcatca cttcgcccaa atttcccgtt aaagtcaact
     2941 gaggagattt acttttgctt agactcgact caatatagag aatttcgccc cccacttgtg
     3001 tccatgctaa accggtaaca accccggcat aatcgtttcc ctgatatttt tcttttgtat
     3061 atcgcggaac tcccaaaaaa gttttcaaat catctacagt aagttgcaca ttataaggct
     3121 cattggaagc cacttttttt gcaattttcc gcatgagcga agcaatttgt ctgtccaatt
     3181 cgcgaacgcc cgattcacgc gtataatttt cgatcaaatg ttcgattaca ggcttcgtca
     3241 aaatcaactg ttcttttgtt aaaccgtgat tttcgagctc tttgggaaca agatggcgtc
     3301 gggcaatttc cactttttct tcctgtgtat agccacttac ttctatcaat tccatcctgt
     3361 cgagtaaagg acgaggaatg gtattcagat tgttggctgt agcaataaac atcactttcg
     3421 acaaatcgaa atcgacgtcg agataattat cgtgaaaact ggtattctgc tcggggtcga
     3481 gaacttcaag caaagcagac gacggatcac ctttataatc ggcagcaacc ttgtcaattt
     3541 catccaacac aaaaaccgga ttggccgact gtgctttctg aatattctgc ataattcttc
     3601 ccggcaacgc tccgatataa gttcttcgat gaccgcgaat ttcggcttcg tcatgcaaac
     3661 caccaagcga aattcttaca tattttctgt tcaacgctct ggctatggat ttgcccaacg
     3721 aagtctttcc ggttcccggc gggccataaa gacaaataat gggcgatttc atgtctccct
     3781 tcaatttcag cacggccaaa tgttccaaaa tacgttcttt tacggtttcc atgccgaaat
     3841 gatcctgttc aaggatttta cgtgcatttt tcaaattaaa attatctttt gtgtactcat
     3901 tccatggcaa ttccaataaa gtttccacat aattcagctg cgtagtataa tccggagaat
     3961 gaggcgacat acgttccagt tttttcaatt ccttctcaaa caaagcagcc acattttcat
     4021 tccatttttt ttgttttgct ttttctctga aatccagaat atcctgttcg ggagaagctc
     4081 cacccaattc ttcctgaatg gttttcattt gctgctgcag gaaatactgc ttctgctgct
     4141 gatcgatacc ttctttggct ttattttcaa tagaaatttt tatttccagc aattgccatt
     4201 ccttgttcaa caaatcgagc aattgatagc cccgatccag aatatcgtca atttcaagca
     4261 gtctttgctt ttcctgtaca ttcaatccaa aattcacaca tacataatta ataagaaaaa
     4321 cggggttatc aatattacgt atggcaaaaa ctgcttctgg aggcattatt cctgcattgg
     4381 ctataatttt tgcagccata tccttaatgg tagcaaccag tgccggaaaa acaccatctt
     4441 cagcgcttgg aattatttcg ggagccaaat taatccgcgc tttcatataa ggcttcagtg
     4501 tcaaaacttc acccagaaaa aaacgtgatt ttccttcaag aataaccgta gtagtgttat
     4561 cgttcatttc caaaatccgt acaactttag ccacagttcc catagaatac aactggtcag
     4621 gagtcggatc atcaatattt ttatcttttt gtgtgcaaac gcctatataa tgattgcttt
     4681 cttttgcttc ccgcacaagt ttcagcgact tcggtcgagc aaccgatacc ggtaataaaa
     4741 cacctggata caaaaccatg tttctcaagg gtagaatagg caatatatcg cccgactcga
     4801 gagaaacatc tccatttttt tcctcttcaa acgaaataat gggaaaagct tccgaaggtt
     4861 gtgttaattc ttcaatatta aaatcatcaa atgaatgttt cattgcgcta tttaaaattt
     4921 taatgacaaa attagtatct ttaatgtcag aatgacataa atttattcaa attcaacaaa
     4981 atgggtttcc ttcttttcta aaaaatcttc agaaaatggg tcgacgatat ttttttcaat
     5041 tgttgtgcca aaaaatctgt attaaaaacc ggcagttcta aaaatggttt ttcacaaaat
     5101 acccatttaa atctttcatc agaatataat ttattttttt aaaggtctga tgggacacac
     5161 atgtctgaat attatattca ttcgtgtgta tgcactacat gtgggttttc ttattataaa
     5221 attttttttc gtgtgggaga aaaagaaaac aaatgattga aaaaatacga aaaggaaatt
     5281 aaatatatag ttcaggaaat caaataatta aatatattaa gaggttaaaa aattacttat
     5341 tgttgggttt taaagttttg accaaaagcc ttagctgtaa tcgattccac ctttactttc
     5401 catactttca cgtttctaag tgccggaagc gagtatttaa aatcgctgcg ttgtgtatat
     5461 tttttcataa acaaattcat ggcctgttct ttttcttcca aattttcgat aaaaatcacc
     5521 ttccctttca ccagcacact tttggagttc atgctgtaac tgcatgccac atcaggatgt
     5581 tggtacatta atttcctgtc cgaacaaaac gtaatacaga cacgattatc cttttggagt
     5641 aatagctgat gttttccttc aggtgcagaa tgtaaaataa cttcgttatg atcaaaagca
     5701 aaattcatgg gtataacata aggacttcca tccaattccg aaatacctac aaagcaaata
     5761 tcacaggcat tgataatatt ttcaatttca acttggtctt caataaaaaa agttctcata
     5821 taaattctgt ttaaaagaca aatataatca aatttctttg tgatttatca tcgaaatgaa
     5881 actattataa aatcttggca taaaatttgt taataaacaa tagcttcact taagcgaaat
     5941 cctagaaaac gagatagaat agaaaacgtt ttcgtaaaaa aatttttttt tcacactgca
     6001 actaaaagta attattcaaa agtccaacaa aactcaggca aaaacaagaa actaataatt
     6061 ttactttatt gaatacgaag tatttaattg tttttgagag ggcacaaaat atttagaaaa
     6121 ataaaaatga aaaaaattga aaaaaatatc gcaaatactt gcatttataa agaaaaaaag
     6181 atgtaccttt gcatcgattt tgaattgtaa acaaacatgt aaaacttcaa aaaattaaga
     6241 aaatgaaaaa attattttta ttgtgtgctg ttgtatttgc attcgttgca tgtggaccaa
     6301 aagaaaacaa agaagctgct gctactgaag attctgtagt tgttgttgat actgttgttg
     6361 ctccggttga tactgcagca gttgacacaa ctgtggctcc tatgtaattt taaaacaacc
     6421 aaaatttaca gagctgaagt ccgttagtgt tagcggactt ttttcgttta tacatgtagc
     6481 gaattataaa aaactcaaat cccactctta cttctttacc tgaataagaa attatatgta
     6541 aatttgtaaa ccgattgtca taataaagaa aaaaattgtt tatgctaaga attgcagttc
     6601 aaaccaaagg aagacttttt gatgacagca tgtcgttact taacgaagcc ggtattaaaa
     6661 tagaattggg aaaacgtctt ttgcttattc cggcacaaaa ttttccttta gaagttttgt
     6721 ttttacgtga tgacgatatt cccgaatctg ttgccgatgg cgtagccgat attggtattg
     6781 ttggagaaaa cgaatttgct gaaaagcaaa aaaatgtcaa acttattcgt cgattgggat
     6841 tcagtaaatg ccgcctctcg ctggcagttc ccaaagaagc agaatattct gatatctgct
     6901 ggtttgaagg caaaaccata gcaacttctt atcccgtaat tttgagtaat tttctgaaag
     6961 aaaaagggat acgtgctgag attcatgtta taacaggttc agtagaaatt gctcccagca
     7021 taggtcttgc agatgccatt ttcgacattg tcagctcagg aagtacactg gtaaccaatc
     7081 atctgaaaga agtagaggtt gttatgaagt cagaagcctt gctgattgcg aatccaaaac
     7141 tctctgaagc gcaaaaaaag atactggacg agttactttt ccgcatagaa gccgtaaaaa
     7201 tggctgaaga caaaaaatac gttttgatga atgttccaac ctctcagctg gaaaaaatta
     7261 tcgaaataat tcccggtatg aaaagtccta ccgtcatgcc attggctaaa gaaggatgga
     7321 actctgttca cgcagtaatt gccgaaaaag aattttggga tattattgga aaactgaaag
     7381 cttgcggtgc tgaaggaatt ctggtattac ccattgaaaa aatgatcctg taaatttcta
     7441 tcgaaatatg aatctttgca tcgatcaggg aaactcaagt atcaaaatgg gaattttcga
     7501 ccaaagcaaa ctggtaaaaa cttttgtata taaaaatttt gatcgaaaat ctgttttatc
     7561 aatgctccgt aattatcctg tcgatcggtg tatttactcc agcgtaatca ataaaaatgc
     7621 ttcagccgtt gcatttttaa agaaaaatat tgcgcaagta ttactgctga cacatgaaac
     7681 tcccttgcct atcgaaaaca aataccaaac gcccaaaact cttgggaaag accggttggc
     7741 agctgttgtt ggtgcaggtt ttctcgagcc caatcgtgat gttttggtaa tcgatgcagg
     7801 tactgccata actttcgatt tcatggatgc caagagaaac tattggggtg ggaacattga
     7861 tgtcggcttg gaattgagat taatggcgct gcatacattt gcaaaaaaat tgcctttggt
     7921 gaaaccggtt caggaagttc ccttaatggc ctccaatact gaaatggcat tggctgcagg
     7981 cgctctgaac ggaataatat ttgaaatcga cggttatatt aatgctttga aaatcgaata
     8041 tcctgacctt tcaacatttt taacaggcgg aagcttgatt ttttttgata aaaaattaaa
     8101 aaatagcatc tttgtagaaa aaaatttggt gttaatcgga ctcaattata tacttcaata
     8161 taatgtacaa taaaaagatt ttaattgcat ttttaatatt gtttttatct atgcaggata
     8221 cttccagtca aaacaataca aactcaccct atacgcgttt tggttttggt gaaatcaacg
     8281 acaaccacaa cagcgaacaa agagcaatgg gaggtgtggc tttgggggtt cgttctgttt
     8341 cgagtatcaa ttctatcaat cctgcatcct acagtgtggt tgacagtatg actttcatgt
     8401 tcgatttcgg agtttcggcg ttacgctcgc aatttaaaga tccaagtgga aaaagttcaa
     8461 actttactgc caatctcgat tatatcacca ttcaatttcc tctttcaaaa aacatagctt
     8521 taagtgccgg cattcagcct tattcgtttt cgggctacaa tttctatact acagaaatac
     8581 ttaccatacc aaataatagt aatactccgg acagccttaa tgtaacgaaa agttttaccg
     8641 gatcaggtgg attcagtgaa gtttatatgg gattgtctat cgattttttg caacattggg
     8701 cattgggggt caatgcttat tacatgtttg ggacaattaa caattataga aacataagct
     8761 tttcttccac taattacacc tctactacag aattgaatta catagaagcc aataatttcc
     8821 gttttcgtta tgggatgcag tattttactg gtttcaacaa aaaaaacgat ctaaatatcg
     8881 gtttaatatt cgaacctaaa aagaatttta acgtcaagtc ttccaaaatt aattcgggag
     8941 tattgaccga cacaaccaat tatgacacag attttgatct tcccatgcta ctgggagggg
     9001 gaatttatta tacctacaac aaaaagcttt cactcggcat cgactatact ttccaaaatt
     9061 gggccaatac aagattctac ggcaaaacag acagtttggt caatcgaaac aaaatttcca
     9121 ttggagcgga atatttgccc aactcttccg ggagaaagta cagcgaccat atacgttatc
     9181 gtttaggatt aaatgtaaac aacccttatt ataaaattga cggaaaatcg cttgataata
     9241 attttggtat aacttttggt attggattac cgttacccta cagccgaaca atgctaaaca
     9301 cttcctttga atatggaaaa attggtaatt acagttcttt gagggaagac tattttaaat
     9361 ttacatttaa tgtttgcgtc aatgagaatt ggttctttaa aagaaaatta taaaacaaac
     9421 aaaattttaa aagtcataaa aaaatgaaaa caaaaatatt aatgttgttg ttggctgcaa
     9481 tattctcaac caacacgttt gcccaaaaag gcaaaaatgt tcaaacatcg gctactacag
     9541 aaaacagttc agaacaggta accgattcca tgtgtttgat ttacattagt ctttttaacg
     9601 aagctgcaaa aaacaaacag tatgcagatg ccataggccc ttggactttg gcttataatg
     9661 aatgccccgg agcaaacagg gtaatttata cacgaggaag agaaattttg caatggaaac
     9721 tttcacaagc caaagactta acttcttaca aggaagcttt tgatgaattg atggccatgt
     9781 atgacaaaag aataaagtat tttggtgatg acagccgata tccaactcct tggatacttg
     9841 gggtaaaggc tttggattat attacttacg taaaaggtga tgacttgaaa aaaccggctt
     9901 acgaatggtt ggaacaatcc atcaacggac tcaaggaaaa ttctgaattg gaagtacttc
     9961 ggcaatttat aatgctttcc tataatcttt ataaagcaaa tcctgaacat gccgaaaaat
    10021 ttattgccga ttatttaaaa gttaacgata tacttgaaac tcaaattgct aatccagaat
    10081 ataaatccaa cgagctggcc aatcaaatca agcaggggct ggatgtgttg tttgcacaaa
    10141 gcggtgcagc agattgtcaa atgctggaca agatgtatgc ctcaaaagta acacaaaatt
    10201 ctaccaatct agaattttta aataacgttt taagtttcta caatcgcata ggatgcatac
    10261 aatcagatgt ttatttcagt gctgcattgt ctgctcataa aattcaacct tcaactgaat
    10321 cggcaaatgc attggcagaa atgagttata aaaagaaaga ttattccggt gccattaaat
    10381 tttatgaaca agccactcaa ctagcaaaca acaacaaaga gaaagctacc tatcagtata
    10441 aaattgctca aatatactat ggcgacctca aaaactattc tcgagccaga gaatatgcac
    10501 tgaaatcgtt agaattcaat cccaataacg gaagtgccta tttgatgata ggcatcatgt
    10561 acgccaattc caatatctat gatgatccag tattacaaaa atcggtgtat tgggcggctg
    10621 tcgataaatt cataaaagca aaacaagttg ataatgaccc taaaattatt gaagaagcca
    10681 ataaattgat tcaaacttac attaattatt tcccatcaaa agaagatatt ttcttccatc
    10741 ctcaactgga agagggtaaa tctttttttg taggcgggtg gattggcgaa tcaaccattt
    10801 gcagggcaag caattaaaat cattcgaata aataaaataa aggcatccct tagggatctt
    10861 atgaaacaga tattaaaaaa ttatttttca aaaagcataa caactatgtt tatagttgtt
    10921 atgctttttt tatcttttgc atgcaatcga ccaactaaac ctgaaattgt tcaaattgaa
    10981 tctgatcctt ctttgattcc gactcttcat gctacggata ttactaccgt tatttccgat
    11041 tcaggaatta cccgctaccg tatttctgct ccgcaatggg atatttacga taaagttccg
    11101 gatccctatt gggaatttcc ttatggcatt cacttggaac gtttcgatat gaatctgcaa
    11161 gtagatgcca atattcacag caagtatgcc cgtttttttg ttaataaaaa attgtgggaa
    11221 ctgaaaaatg atgtaaaagc caccaatctg aaaggtgaac tttttgaaac cgaacaactt
    11281 ttttgggatc agaacagtca aaaaatttat tccgacagtt tgattaaagt tactcaggca
    11341 acccaaatca ttaatgccat tggatttgaa tcggatcaat ccatgaatta ctatgttttc
    11401 cgcaatgtta agggaatttt ccctgtggat gaaagcaaag atactgttcc gacttcccag
    11461 agcagtaatg ccggcacacc gattagagca atgaacgcca agtaaaaatt ttccaaaaat
    11521 ttgttattcg gaaaattcaa gcagcagcta tccgccgaat ttcttccata atggcgtttg
    11581 caaaagcttc agcctgttct ttggttttgg cttcggcgta aattctgata atcggctcag
    11641 tattcgattt gcgtaaatgt acccaaccgg taggaaaatc gatttttaca ccatcaatat
    11701 cattaacttc gtactgaagt tgataattgt ttttaatggt tgcaagaatt ttatcgacat
    11761 caatagacgg cgtcaattca attttatttt tagaaataaa atactgtgga tactcatttt
    11821 tcaatgcaga cacagttttt ttcgactttg ccagataaga aagaaaaagt gcgataccta
    11881 ccaacgcatc gcggccataa tgacttgcag gataaataac tccgccatta ccttcaccac
    11941 caattacagc attcgtttgt ttcattaagg taaccacatt cacttcacca acagcagcag
    12001 ctgaatattt tccaccccgt ttttgtgtaa tatcgcgtaa agctctcgac gaactaagat
    12061 tggaaacggt atttcccggt gtatgctgca atacataatc tgccacagcc accaacgtat
    12121 attcttcacc aaacatgctg ccgtcttcac aaataatggc taaacgatcc acatcaggat
    12181 caaccacaaa acctacatcg gctttagatt cttttaccaa cgacgagatt tcagacaaat
    12241 gttcgggaag cggttcggga ttgtgagcaa aaattcctgt cggttcgcaa ttgagtttaa
    12301 taactttttt aacgcccaac gcttcgagca aagccggtac tgcaattcct cccaccgaat
    12361 ttactgcatc tatggcaacc gtaaaatcag ctttttttat agcatcaact tctactaaat
    12421 tcagcgacag aatattatca atgtgtttct gcaaaaaagt aaaattttcg gaaatttttc
    12481 ccaactgctc aacagaagca aaatcgaaat tttcctcggc cgctattttc aatacttctt
    12541 caccttcctt cgcattcagg aactcacctt tttcgttaag cagtttcaaa gcattccact
    12601 gcattgggtt gtggctggca gtaagaataa tgccacccaa agctttctcg taaatgacag
    12661 ccaactctgt agtaggcgta gtggccaatc caatattaac cacctccagt ccttgacccg
    12721 ataaggtacc ggctaccaaa tgatatacca tatctcccga aattcgggca tcacgtccca
    12781 ctacaatttt atttgaatca ttacctgcat tttgtttcat aaatttggca taagcagccg
    12841 aaaaccgaac gatatccaga ggtgttaatc cctctcctgc tcggccacca atagttccac
    12901 gaattccgga aatagatttt atcagtgcca tataaaactt aattagaagt ttgttaaaaa
    12961 attaatagct cttttgaatg ccaacaatct taaaatcgta tcccagtgga gttacgctgt
    13021 ttatatattc gctcaagcct atttttgaag gaacaataag ttttgcttca gaattgttgc
    13081 gtttcatata ggaaacggct tcgtgccaag ccgtacaggc ttgcgtataa tcaagataag
    13141 tgaaagaaat cggataagga tagtcaatgg tattccagta ataaatggta tcgggatatt
    13201 cggtcagtgt atattgtcga taacgaataa caacccgatc attttttgct accgtgtcgg
    13261 cgttggcagc agtatcgccc ggactgacca aatgaaaata tagaccactt gaagttttca
    13321 catatttatt atcccattct ccatcagcag gaaaagttgt aactacctgt atattactac
    13381 gtttaatata acttgctata atggcttttt catcctttaa aagctcggcg taagttttag
    13441 tgttgttgca cgaagaaaaa attaggataa caggcaaaaa acccagaaac aaaaaaatga
    13501 attttttcat aaaaccgtat aaattaattt tatacaaatg taccataaaa aagtagaaag
    13561 aaaaagttaa gaaactcaaa tctatggatt gaaaatccat aggttggatt gcaaataaaa
    13621 ttcgctttaa ttttggctat aagtcaatta aaatgttaaa ttaatgttca taaacttatt
    13681 taaaaatgta attttcatta gaattggatt gttttttgtg atccaaatag gaatttatca
    13741 tctcatccgt taccttgtct gtactcgaac atccacaacc ttgcgtataa aaatgattaa
    13801 tccaaccccg actatgccgt tcctgaaatt tcctgcaaca acatccctga acttttccct
    13861 tttaatcgct ttttatatcg cttaaactta tttgaaatgg ctggcttaaa aaaacatctt
    13921 ccttactcac cacttctttg aaaatctcta cttgttcccg ttgcatattt agcgtaaaat
    13981 atctcaacaa cgcagtggtt ttccgttgaa atactgcata aacatctttg taatcacact
    14041 ttatgaatat gaacttgtaa actgtatatg ctattaccgc tttcctgttt attcatctca
    14101 tttttatggc aaatttaata gcagttttca tcagaatata atttattttt taaaggtctg
    14161 atggaaccca catgtctgaa tattatattc atttgtgttt gtgtgtgcaa tacatgtggg
    14221 tttcttaaaa ctaacgtttt tgaaaaaatt tgaaataagg aagcataagt tattgcctat
    14281 ggttgactgt atattaaatc aaaacaaggt caactgactg ccctcttcgt cagttgagtg
    14341 aatctccggc ctgtcttcgt ttatagcatt tgaaacggca tcttcttttg tttgggaatt
    14401 tacgttggtt ttcctttcaa catcagttgc cttttccggc gactcttgtg catcagaaga
    14461 atttgatatt cgatcgtttt ccgaagcgtt ttcgttatct tcttcctcat cgctgttgat
    14521 taccgtactt tcttcattat tcttttcttt aaaccgaaca ggctccaatt cgttaacagt
    14581 ttctacttca aaagttgtca aacgttttcc tttggctttg taacttttta cactgataaa
    14641 ttcttcagca tccactatca acgggtttct gtgggcatcg tgtccaccaa aaacaacctc
    14701 aaatcttgga aagtcgacac tggaaagcag aatcaatcga gattctggat gttcaccaac
    14761 aaagctgttt tttttctgcg atggagtaag ttgaaatcgt ttcagataat aattttttaa
    14821 ttctccgtca tacaccactg ccgaccaaac cttattttcg tcaaattttt caataatcag
    14881 aatattgttt tcgtaatgat tggcagcatc aaaattcgtg gtgtagtatt cgccgttttt
    14941 tgaaacaata agaatcaggt catcgctttg aaactctccc aaatattctc catgtttgtc
    15001 gtaattcaat cgtaaaacat catggtcgaa ccaaacctga cgtcctccga gcgtagaacc
    15061 tcccttctgt ttgagcgtaa ttttatgaat ttcgtttttg gtcagaatgt ttcccattgc
    15121 ctggcgacct tttatggcaa tttctccaaa atctttttca aataccagct tggtcatgcg
    15181 cggtttgggt ttcaacgtta cacgaattac ttcggcttcg gcattgggat tggctgaaaa
    15241 atacaaaatt tttgaaccgg cagaaccctg cgtcaaatcg tattccttgt cgcgagtaat
    15301 gccgttgacg gcaaaacgtt tgatgtaata tggtccggct ttcccatctc tgtacaccac
    15361 gttataaatc gttcgtttgt catttttttt gaaaacattg acatagagaa tatttttccc
    15421 gacaaacatt ttttcggcaa ctttggtaac cttgtatctt ccatccttga aaaacacaat
    15481 aatatcgtca atatctgaac aattgcaaac aaattcatcc tttttcagcg aagttccaat
    15541 aaaaccttct tcacggttta catatagttt ttcgttggct tcggccactt tggttgcgat
    15601 aatggtttca aaattacgaa tctccgtgcg ccggggataa tttttcccgt aacgatcctt
    15661 taaaccctga taccaagaga tggtataatc gattaaatga tttaagtgaa aatccacttc
    15721 atcaattttt ttctgaatag ctatcagcgt atcgttagct ttgtcggaat caaatttggt
    15781 aatgcgaacc attttaatgt cgagtagtct caatatatct tcccttgtta cggctctgat
    15841 aaaattttcc ttgaagggtt ccaaacgttg gtcgatataa ttgactacgg catcctgact
    15901 tgagctgtct tcaaaacctt tatccttgta aattctgttt tcaataaaaa ttttttccag
    15961 tgatgtaaaa aaaagctgct ccaaaagttc ctgcttctga atattaagtt cggcttccaa
    16021 taaatctttg gtatgatcgc agctgacgcg aagcatttcg ctgacattca taaaccgcgg
    16081 tttccggtca tgaatcacgc agcagttggg cgatatgctg atttcacaat cggtaaaggc
    16141 atacagcgca tcaatggttt tgtcgggtga agttccgggt tgcaaatgaa ccaatatttc
    16201 gacgctggac gcagtattgt cgtcaacttt cctgattttt atttttccct tgtcgttggc
    16261 ttttaaaatg gaatcaatca gttttgatgt atttgttccg taaggtattt cggtaataga
    16321 caatgtttta ttgtcaacct tattgatttt agctctgatt ttaaccactc ctccgcgttc
    16381 gccatcattg taatggctga cgtcgataag tccacctgtt tgaaaatcgg gatacagttc
    16441 aaaatcttca cctttcagat aagcaatgca cgcatcaatc aattcgttaa aattgtgagg
    16501 caaaatttta gaattcaatc caactgcaat tccttccacg ccctgagcca gtagtaatgg
    16561 aaatttcacc ggcaaattca ccggttcctt gttccgaccg tcgtaagata gtttccactc
    16621 cgttgtttta gggttgaaaa cggtttcgag tgcaaatttg gaaagacgtg cctcgatata
    16681 acgaggtgca gctgcagagt ctcccgtgag gatgtttccc cagtttccct gacagtcgat
    16741 aagcaaatct ttctgtccca gctgaataag cgcatctcca atggaagcat ctccatgcgg
    16801 atgaaattgc atggtatgcc ccacaatatt ggccaccttg ttgtaacgac cgtcttcgag
    16861 tcttctcata gaatgaagaa tacgtcgctg cacaggtttc agtccatcgt aaatatcggg
    16921 tacggcacgt tcaagaatta cgtaggatgc gtaatccaga aaccaattct gatacatgcc
    16981 ggaaagatga tgttttactt cactgttttc tattttcggt attttataag aagaatgccc
    17041 ggaaggtttc gtctctgaat tgtcttcaat atcggcctgc tgaacgaaat cttcctcatt
    17101 ttccgctccg gttatttctt ccctgtcatc tctttcgtcc atatattaat ttactccatt
    17161 tttttcctaa gttctatcta aagttttctg gaagtgattt tttcttaaaa tttcacgaat
    17221 tacatgagaa gccatcatac agccaaaaat ggcaggcata tacgaaatgg ttcccacagt
    17281 agatttttta aacttttcat cttcaacagg taatacggca tttttgttgg gcaattcatc
    17341 cgaaaaaaca acggcaaggc cttttgttat gcccattttc gacagccgct gccgtacagc
    17401 ttttgccaat ggacaagtat gcgtattgga aatatccgta attctgactt ttgaaggatc
    17461 cattctgcct cccgaaccca ttgaggaaat aatatcgata ccccgctcca aacagctctt
    17521 tattagaaaa actttgggcg taaccgtatc aatggcatct accacaaaat cgtatttctc
    17581 tgtttcaagc aattcttcga tgttcgatac ttctgcccat tgattaaaag tttttaattt
    17641 taaatcagga ttgatttgca acaacctttg ggccatcaca tcggttttgt attgcgaaat
    17701 cgtgtttaca gacgccagta tctgcctgtt taaattggta ggattgatgg tatcgtgatc
    17761 cacaattgtc attttgccta taccggcacg gcaaatcatt tctgcagcca ggccacccac
    17821 accgcctaaa cccactacga gcaccgatgc ctgttgcaag gcttccatgc ccgactttcc
    17881 aatcagtaac tctgtacgtt gtgtccacga agtcatatgg gtataattgt catacaaagt
    17941 taaacttttg cgaggacaaa tccatacttt tggaagagaa attttttgat cggtttgaat
    18001 aaaatggagt agagttgcaa gtttgagacc aataacctca aattgaaaga atttggatta
    18061 gaaggttata gtagttatat gttatttgtt acattctttt atgcatgttt acagagttaa
    18121 attttagttt ttgaatttag aaattaagtt ttccaaaaaa tatcgataga taccataaat
    18181 taatttatac ttttaatttt aatctcatgg cattgcgaaa aatactgctt catagtttcc
    18241 cgatttactc gctttcgttc ggcatcacaa aaccaacccc atttgttgaa atattttatc
    18301 atattgataa tgtgaatcca caacatccgc aaatttttat aagaaccgcg ttcgtgataa
    18361 tgtaccaccg aaacatgtgg ataaaaaacc gtccgaaatt ttcggcgtat tctacgcgtc
    18421 agatcgatat cttcaggata cagaaaaaaa cgttcgtcaa acaggccaac ttcccgaagc
    18481 gcatcggtac gaaaaaacat gaaacaaccc gaaagatagg gaacatccat cactttatcg
    18541 tagccggaag ctctcatttc aaatttttcg gttcgttttc tcgtccagct tttgggcaaa
    18601 aaacgccgaa atatcaaatc gaaaggagaa ggaagcagtt tacaaaggt
//
LOCUS       DAOSIB010000039        18641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_3208_length_18641_cov_12.453567, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000039 DAOSIB010000000
VERSION     DAOSIB010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18641
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3208_length_18641_cov_12.453567"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="PLD44_04985"
     CDS             complement(<1..1349)
                     /locus_tag="PLD44_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98723.1"
                     /translation="MKNKIFYISVFCSFFACFVQADNIGMGKWRMHLAYNSVGQIAQS
                     DKKIYAVSDGALFSVDKLDGSMEFYSKISGLHDTSVSRIEYDQNTRQLLVIYANGNID
                     FINEGGVINLPDLYNKQMTFSKDVQHITFFGNRAYLSCHFGVVALNMVKKEIAETYFI
                     GADAAAVDVLCTAVHGQTIYALSKSAVYKADVTNPYLVNYEVWSTVANLPGSGNFQSV
                     VSFANQLFLLRNGKIYQMNASGQWSPFLSQVTASNLLVSDGSLFILSTNNNIYLLDNQ
                     LVSKTISGLGAVADAEYDSENNTCWFAANGNGIISYRQEGSLLPVVSAYKPSGPAVNI
                     PWQMRFSGQKLFVVPGGRSSAQYNQQGYVMMFENGVWTNIDGKTIETQTGKRVLDFMD
                     IAVDPNDPTHFLVPSYGTGIYEFKNNQFSKWYNFTNSLIETIFPNDPNGSYLYMRMDG
                     "
     gene            complement(1562..2182)
                     /locus_tag="PLD44_04990"
     CDS             complement(1562..2182)
                     /locus_tag="PLD44_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008023629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HOF98724.1"
                     /translation="MSLKIQKNINIYLKVEKLVFATNNLHKLQEVKAILDGKVQIQSL
                     SEIGCLDEIPETADTLEGNALLKAKFVYDRFQLDCFADDTGLEIEALGGEPGVFSARY
                     AGLEHDFYKNRMKVLRLMEQQTNRKAQFRTVIALVLQGKEYLFEGKVEGIITREPRGE
                     GGFGYDSIFVPNGYEQTFAELSAEQKNQISHRAHAVKKFADFIVSR"
     gene            2297..3658
                     /locus_tag="PLD44_04995"
     CDS             2297..3658
                     /locus_tag="PLD44_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOF98725.1"
                     /translation="MAKKERINFSKIGAIAAATGSAVGLGNIWRFPYLLGESGGGAFL
                     LVYILCVVFLGLPVMISEFSIGRMAQANAAGSFRTLAPKSKWWLVGLMGVISAFLILG
                     FYVVVSGWTLEYIVQALSNKFANASTAQLNQVFTDFSTNTYRPVGWMLLFTALTCFII
                     IAGVKNGIEKSTKLMMPLLFLIIIILGIRSITLPTGTEGLKFLFKADFGKINSEVILS
                     AMGQAFFSLSLGMGCLITYGSYINKDNNLSSTALQVTIFDTLVAVLASIAIFPAVFSF
                     GINPAQGPELVFITLPNIFSRLHGGYIWAVMFFVLLALASLTSTISLLEVIVAFLTEE
                     LHISRAKAAIVSSLGIALLGILSSFSLGIWKDFSLFGYGFFALFDSITSKILMPVGGI
                     FISLFVGWRIDKQKILLELSSNGKYKIPFLNIFLLITRYLAPLAILLVLLNQLGLTKW
                     LGF"
     gene            3740..4450
                     /locus_tag="PLD44_05000"
     CDS             3740..4450
                     /locus_tag="PLD44_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007173617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF98726.1"
                     /translation="MIEASNIHKSFGNLEVLKGVDLSVDKGEVVAIVGPSGAGKTTLL
                     QILGTIDKPNDGVVSINGVTFSKLSEKELSAFRNRNIGFVFQFHQLLPEFTALENVMF
                     PALIAGKDVKKTTNKAKKILEELQLADRMQHKPNELSGGEKQRVAVARALINDPLLIL
                     ADEPSGSLDSKNKTELHKLLFQLRDKLGLTIVIVTHDMELALQTDRVIELKDGMVLKT
                     IEKEKFVVSPGIEPESKV"
     gene            complement(4413..4484)
                     /locus_tag="PLD44_05005"
     tRNA            complement(4413..4484)
                     /locus_tag="PLD44_05005"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4449..4451),aa:Arg,seq:cct)
     gene            4583..5224
                     /locus_tag="PLD44_05010"
     CDS             4583..5224
                     /locus_tag="PLD44_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HOF98727.1"
                     /translation="MSKNKFFVVWKGKQPGIYSSWEECKRQVHQFEGALFKGFATEEE
                     AKKAFESLPWKYIDIRNNAQNKSSGKKGKKTSIGQPIMESISVDAACSGNPGLLEYRG
                     VYTKTGKEIFHQGPFRNGTNNIGEFLAIVHALALMKQNNSQLPLYSDSRTALKWVQQK
                     KAKTKLEKNEENKSLFELIERAENWLQNNDYSTPLLKWETEAWGEIPADFGRK"
     gene            complement(5226..6617)
                     /locus_tag="PLD44_05015"
     CDS             complement(5226..6617)
                     /locus_tag="PLD44_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98728.1"
                     /translation="MNKKLSLGTRFVLLSIIVFFVFIALTGLIYRYTAIGIEQNIPVL
                     TLLAGSYFFVQLILFLVIFKSHLPKWNFSWDNILTVSLFFLLISTVLSVNVSITATVL
                     FVVLAVLFAIVKKKHFQPHPLFYFMGAYYLFQYVGLLWSIDTAYGWRFVGRGLSFIVL
                     PLAFGFYSVSEEKKNLLLRIFFRFLEVYLLMVIVAYFYQVEFHKVDFGVGFHLKKNYF
                     CTPVPPGVDYYLLLGWAGYNHPTFVSFILTLMFGIGFYLWRNDNSPQTVKISGFEMGF
                     YGFMTAVVILLLQSRIGMIMFPFGIFLTLLYNLRKKKKLVLSVIVFSAFLVLSAGFYI
                     YKNYRGYFYDRPREIQTDIVLSFLKDHYLLGTGTGGMKVLIPWFPTAHNQFLGDLFHL
                     GIPGLLLLLSLMGSSFYFACKDKNFLLLYFLLIYFVMMQIEMPLSLQKGITYFTLFTG
                     LLLKPRPFGKETK"
     gene            complement(6626..8119)
                     /locus_tag="PLD44_05020"
     CDS             complement(6626..8119)
                     /locus_tag="PLD44_05020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOF98729.1"
                     /translation="MENPLVSVIIPVYNMEAFLAEAIDSVLASTYPNFEIIVVDDGST
                     DQSPVIAEHYSERDTRIRFYRQSNRGVAAARNLALQEARGEFVLPVDADDKISEIFLE
                     TAAKVLVQLPQVKIVTCEYEYFGEKQGKGKYRPYHRKLIARKNVINNCSMYRRRDALK
                     VGGYCEEIIGREDWEFWISLLKNGGEVEHLSETGFYYRIRSNSKRITDRKLKKHIIDV
                     LNRRHKAFFYRELGGKLHYRRSLSRLLNFFYHCYKPETFFVHPDFKEEEEFFYSLPDI
                     FDNQGETIHSGRNTIKIFNAGNRKWVVKSFQSPNVVNRFIYGIFRKSKARRSYEYALK
                     LLSLGIATPQPIGYYEQKIFFLFNKSFYVSESSECNHRFEELIRDVNFPDREKILKAV
                     ARFTAELHEKGVLHSDYSAGNILFSQTNDDIHIELIDLNRLKFGKISKTAGCRNFERL
                     NLEPEALKTMAEEYAVARGFEKQECVELVQRYRWKKHKNISSLQPKN"
     gene            complement(8124..>8670)
                     /locus_tag="PLD44_05025"
     CDS             complement(8124..>8670)
                     /locus_tag="PLD44_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOF98730.1"
                     /translation="GFRLVNLKDITTHPSPNYLLVLQLSQQEALSEHAHLLVVESDVI
                     VKKNTIQILFDAAQKLENPGLIAAVTHDEEGNINFPYLYAKNFDSGTVQTSKRLSFCC
                     TLLSNSFLEAFDFKNLNPEKSWYDVFISHKAVELGFNNYLLTYLPVLHLPHSSRPWKQ
                     LKYTHPLKYYWLKYTKGRDKI"
     assembly_gap    8671..8770
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8771..10014)
                     /locus_tag="PLD44_05030"
     CDS             complement(<8771..10014)
                     /locus_tag="PLD44_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOF98731.1"
                     /translation="MKRLAKDTAIYGLSSIIGKFLNWCLVPLYTYVLESSADYGIVTN
                     LYAWTALLLVILTYGMETGFFRFANKNLDDAPKVYGTTMISVGFTSLLFATICVIFSQ
                     PIANGMGYPKYPEFIAMLAIVVSMDAFDAIPFAYLRYKNRPIKFAALKLFMIFTNIFF
                     NIFFLVICPWLQKKAPGLISWFYNPHYGVGYIFVANLISTAAVTLALLPNMLEAKFRL
                     DMALLKKMLQYSLPLLLLGIAGIIDQTIDKIIFPFLFEDKAYAAAQLGIYGACFKISM
                     VMMMFTQAFRYAYEPFIFAQYKDKNSTAAYADAMKYFIIFSLMIFLGMVFYLDIFKFI
                     IRSDYWAGLRVVPIVLFSYLFQGVFFNLSLWYKLTDKTMYGAWISIVGSLITIAINII
                     FVPVFSYMASAWASFACYFVMML"
     gene            complement(10082..11095)
                     /gene="ruvB"
                     /locus_tag="PLD44_05035"
     CDS             complement(10082..11095)
                     /gene="ruvB"
                     /locus_tag="PLD44_05035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOF98732.1"
                     /translation="MTENFDIRSNNIEKDFENALRPLTFSDFEGQNKIVENLKVFVQA
                     ARMRSESLDHVLLHGPPGLGKTTLSNIIAHELNVGIKITSGPVLDKPGDLAGLLTSLE
                     PNDILFIDEIHRLSPVVEEYLYSAMEDYRIDIMIDKGPSARSIQLELNPFTLIGATTR
                     SGLLTSPLRARFGINCHFEYYDTEVITHIIKRSAYLLNVQIDERAAIEIGSRSRGTPR
                     VANALLRRVRDFAQVKGSGKIDLEIACYALEALNIDKYGLDEIDNKILTTIIDKFNGG
                     PVGLSTIATALGEDAGTLEEVYEPFLIMEGFLKRTPRGREVTELAYRHLGKSKMGMMD
                     TLF"
     gene            complement(11159..12070)
                     /locus_tag="PLD44_05040"
     CDS             complement(11159..12070)
                     /locus_tag="PLD44_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF98733.1"
                     /translation="MSINITKLSKNFGQQKVLDDVCFDIDKNGVVGFLGPNGAGKTTT
                     MRIIAGALPYETGSVKVCGHEVKDAPLKIKSLIGYLPEQNPLYEEMYVKEYLLFIADV
                     YKLGQYRNKKVDEMIDKVGLRAEFRKKIGQLSKGYRQRVGLAQALIHEPKVLILDEPT
                     TGLDPNQLEEIRTLILELGKDRTVLLSTHIMQEVKALCNRVIIINKGKIAADFEELSS
                     IHSLNRDIFQVEVEFFKKPAAKDIENIPGVLKVNFLDETRLLIVASEDIRSEIFDFSV
                     KQGNKILLMKLVEQEIEQVFRSLTAES"
     gene            complement(12218..12886)
                     /gene="pssA"
                     /locus_tag="PLD44_05045"
     CDS             complement(12218..12886)
                     /gene="pssA"
                     /locus_tag="PLD44_05045"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOF98734.1"
                     /translation="MKKHIPNFLTCLNLFSGCIGVYYAFQGNYFAVLVFILIAAFFDF
                     IDGLAARLLQAYSPMGKELDSLADVVSFGLAPGAVCFSLLSQSSLPFWVAFAGFVITL
                     FSALRLAKFNIDERQGSSFLGLATPANAIFWVGLGYSFSEFLTANSWLILILIVIFSA
                     LLVSEIPMFSLKFKNLKWEENELQYIFLMLCVLFLFVFQLNAFVPIIIAYLFLAVVKV
                     VFKI"
     gene            complement(13019..13465)
                     /gene="dtd"
                     /locus_tag="PLD44_05050"
     CDS             complement(13019..13465)
                     /gene="dtd"
                     /locus_tag="PLD44_05050"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOF98735.1"
                     /translation="MRVLVQRVTEASVEIDRLIISSIGKGLLVFIGIESTDTLQDAEW
                     LAGKTAALRIFDDEKGVMNLSVTDIDGEVLVVSQFTLCAETRKGNRPSYIRAARHETA
                     IPLYESFCKIMEQKTGKEVKTGVFAADMKVKLVNDGPVTIWIDSKV"
     gene            complement(13512..14612)
                     /gene="ychF"
                     /locus_tag="PLD44_05055"
     CDS             complement(13512..14612)
                     /gene="ychF"
                     /locus_tag="PLD44_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOF98736.1"
                     /translation="MALQCGIVGLPNVGKSTLFNCLSNAKAQAANFPFCTIEPNVGVT
                     QVPDERINILARLANPQRLVPATVEIVDIAGLVKGASKGEGLGNKFLANIRETDAILH
                     VLRCFEDENVAHVNGVIDPVRDKEIVDAELQLKDLETVESRINKVQKQAQTGGDKQAK
                     IIYEILQEYRSALLQGKSARTVVLDKESKKLVKDLFLLTDKPVMYICNVDEASATSGN
                     AYVEAVREAVKEENAEILVVAAATEAEIAELESYKERQMFLEELGLKESGVSRLIQSA
                     YRLLNLETFFTVGPLEVRAWTYEKGLKAPQCAGVIHTDFEKGFIRAEVIKYEDFVALG
                     SETACREAGKIYIEGRDYVVQDGDIMHFRFNV"
     gene            complement(14834..15400)
                     /gene="purN"
                     /locus_tag="PLD44_05060"
     CDS             complement(14834..15400)
                     /gene="purN"
                     /locus_tag="PLD44_05060"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOF98737.1"
                     /translation="MSKNIALFASGSGSNAENIIRYFTGNSDFSFPLIISNNEKAFVH
                     ERAKKLGVPSLTFPREDFIDGGKILNLLQEKNIDAIVLSGFLLKIPDSIIEAYPQKIV
                     NIHPALLPKFGGKGMYGMKVHRAVLEAGEKETGITIHYVNSNYDEGDIIFQAKCPVLP
                     DDTPESVAQRVHALEYEFFPRIIAEIWK"
     gene            15552..15788
                     /locus_tag="PLD44_05065"
     CDS             15552..15788
                     /locus_tag="PLD44_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOF98738.1"
                     /translation="MSEVEEKVKTIIADKLGVDEAEITPSASFTNDLGADSLDTVELI
                     MEFEKEFGISIPDDQAEKITTVGDAVSYIENAKK"
     gene            15804..17066
                     /gene="fabF"
                     /locus_tag="PLD44_05070"
     CDS             15804..17066
                     /gene="fabF"
                     /locus_tag="PLD44_05070"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOF98739.1"
                     /translation="MELKRVVVTGLGAITPLGSSVPKLWENILNGVSGAGPITHFDAS
                     QFKTQFACEVKDFDPFQYFEKKEIRKVDPYAQYAIAAAKQAIENCGINLETVDKDEVG
                     VIFSSGIGGITTFEQEVSNYFLNKEMGPKFNPFFIPKMISDIAAGQISILYGFKGPNY
                     ATTSACASSTHSIIDAFNYIRLGKAKMFVVGGAEAAITPAGVGGFNSMMALSTRNDDP
                     AGASRPFSKSRDGFVIGEGAGCMILEELDHALARGANILCEVAGGGMSADAYHLTASH
                     PEGLGAILAMKSALKDAGMQVEDIDYINVHGTSTPVGDTSEAKAIENVFGEQAYQLNI
                     SSTKSMTGHLLGAAGAVEAIICTLAIINDVVPPTINFTEGDEDPDINYRLNFTFNKPQ
                     KRTINAALSNNFGFGGHNASVILKKFTK"
     gene            17071..17838
                     /gene="rnc"
                     /locus_tag="PLD44_05075"
     CDS             17071..17838
                     /gene="rnc"
                     /locus_tag="PLD44_05075"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOF98740.1"
                     /translation="MKNFFHLNGLFSKYRKEPYSLFRKLLGFYPRDINYYLLALTHRS
                     LSMTNHDGKVVSNERLEFLGDAVLNAIVSDLFYRYYEQKDEGFLTNIRSKIVNRDSLN
                     HLALKLELDKIVKVSKNVNPQVSNDIYGNALEALIGAIYLDYGYNHCMTFVKTKLFEP
                     FIDLSKVEKFEMNYKSKLIEWCQKNHFQIDFEMSCKTHNENHQPIFLSRVLIEGMPVS
                     ESTGLNKKESQQKASRIAYQQIIEQKTFHFINEESKN"
     gene            17992..18192
                     /locus_tag="PLD44_05080"
     CDS             17992..18192
                     /locus_tag="PLD44_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOF98741.1"
                     /translation="MSTENIGINAGAVWKVLNEKGEMDVKALKKSTKLSEKDLNMALG
                     WLAREGKIEFNETEENLLVHLV"
     gene            18342..18414
                     /locus_tag="PLD44_05085"
     tRNA            18342..18414
                     /locus_tag="PLD44_05085"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18375..18377,aa:Gly,seq:gcc)
     gene            18440..18526
                     /locus_tag="PLD44_05090"
     tRNA            18440..18526
                     /locus_tag="PLD44_05090"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18473..18475,aa:Leu,seq:taa)
BASE COUNT         6047 a         3914 c         3268 g         5312 t
ORIGIN      
        1 ccatccattc gcatgtaaag ataagaacca ttcggatcat tcggaaaaat agtttcgatg
       61 aggctgttag tgaaattata ccatttactg aactgattat ttttaaattc ataaataccc
      121 gtgccataag aaggaaccaa aaaatgagta ggatcattcg gatcaaccgc aatgtccata
      181 aagtccaaca ccctttttcc tgtttgggtt tcaattgttt ttccgtcaat atttgtccaa
      241 acaccatttt caaacatcat gacgtaaccc tgctgattat attgagccga agatcttcca
      301 cccggcacta caaacaattt ctgtccggaa aatcgcattt gccagggtat attcaccgcc
      361 gggccggaag gcttataagc agatacaacc ggcaacagcg acccttcctg acgataggaa
      421 ataattccat ttccattggc cgcaaaccaa caggtattgt tttcactgtc gtattcagca
      481 tccgcaaccg caccaagtcc agaaatagtt tttgaaacca actggttgtc aagcaaataa
      541 atattattat tggtactcaa aataaacaga cttccatcag aaaccagcaa atttgatgct
      601 gtaacctgtg aaagaaaagg agaccattgt cccgacgcat tcatttgata aatctttccg
      661 ttacgtagca aaaaaagctg attggcaaaa gataccacag attgaaagtt accacttccc
      721 ggcaaatttg caacagtcga ccaaacttca taattaacca aatacggatt ggtaacatct
      781 gccttataaa ccgcactttt ggaaagcgca taaatagtct gcccgtgaac tgccgtacaa
      841 agcacatcca cagctgctgc atcggcaccg ataaaatagg tttcggcaat ttcttttttc
      901 accatattca atgctacaac accaaaatga caggacaaat aagcccgatt tccgaaaaaa
      961 gtaatatgct gaacgtcttt gctgaaagtc atttgcttat tatataaatc gggaagatta
     1021 ataacgccgc cttcattaat aaaatctata tttccattgg cataaattac aagcagttgt
     1081 cgagtatttt gatcatattc gattcggctg acagaagtat cgtgcaatcc gctgatttta
     1141 ctgtaaaatt ccatactgcc atcgagttta tctacggaaa ataaagcacc atcacttact
     1201 gcatatattt ttttatcgga ttgcgcaatc tgaccgacac tgttgtaagc caaatgcatt
     1261 ctccattttc ccatgccaat attgtctgcc tgaacgaaac aagcaaaaaa actacaaaag
     1321 accgaaatgt aaaaaatctt atttttcata agttaaatat ttgattttaa cgaaatagcg
     1381 tattaaatta ctaaattacg ctgtattgga aatctttatt tttcaaaaat ttatttatcc
     1441 tcaaaaaagt tgttgaatgt aaaacattaa tccataaaat taaactgaaa acttcctgat
     1501 ttattttgtt ttgtcaatga ttgatgaaaa atgaaaccaa tgaaaaactt tggtttcact
     1561 ttcaccttga aacgataaag tcggcaaatt ttttcactgc atgggctcga tggctgattt
     1621 gattcttttg ctctgccgac aactcggcaa aagtctgttc atagccattg ggaacaaata
     1681 tcgaatcgta tccaaagcca ccttcacctc tcggttctcg agtaattata ccttctactt
     1741 ttccttcaaa aagatattcc ttcccctgta aaacaagggc aataacagtt ctgaattgag
     1801 ctttgcgatt ggtttgttgt tccatcaaac gcaatacttt cattcggttt ttgtaaaaat
     1861 catgctccaa ccctgcatac cttgccgaaa aaacgccggg ttcgcctccc aatgcttcaa
     1921 tttccaagcc tgtgtcatct gcaaaacaat ccaactgaaa tctgtcgtaa acaaattttg
     1981 ccttcaacaa agcattgcct tcaagcgtat cggcggtttc tggtatttcg tccaagcaac
     2041 ctatttccga taaactttgt atttgtactt tcccatccaa aattgcttta acttcctgca
     2101 atttatgaag attatttgta gcaaaaacta atttttccac tttaagataa atgttgatat
     2161 tcttttgaat tttcaaactc atgcaaagat aactatttta caaaaataat tatcaagtca
     2221 gctttctccc attcatttcg atttttattt tttttctcta cttttgcaac aaatatgcat
     2281 cgaaaacaag aaaaatatgg caaaaaagga gagaatcaat tttagcaaaa ttggcgccat
     2341 agcggctgcc acaggctctg ccgtaggatt gggaaacatt tggcgttttc cttatctgct
     2401 tggagaatcc ggaggtggag ctttcctgtt ggtgtacatt ctttgtgttg tttttctggg
     2461 actgccggtt atgatttccg agttttccat tggccgaatg gcgcaggcaa atgctgcagg
     2521 ttcgtttcgc acattggcgc caaaaagcaa atggtggctg gtgggactta tgggcgtgat
     2581 ttcggccttt ttgattttgg gtttttatgt tgtcgtttcg ggatggactc ttgagtatat
     2641 tgtacaggca ttgagcaaca aatttgccaa tgcatccact gcccaactta atcaggtatt
     2701 taccgatttc agtacaaata cttaccgccc agtgggatgg atgctgcttt tcactgcatt
     2761 aacgtgtttt attatcattg ccggtgtaaa aaacggaatt gaaaaaagca ccaaattgat
     2821 gatgccattg ctgtttctga ttatcatcat tttgggaatt cgttccatta ctttaccaac
     2881 cggcacagaa ggattgaaat ttctttttaa ggccgatttc ggaaaaatca attctgaggt
     2941 cattttaagt gcaatgggac aggctttctt ttctttgagt ttgggaatgg gttgcttgat
     3001 tacttacggg tcttacataa ataaagacaa taaccttagt tctacagcgc ttcaagttac
     3061 cattttcgac acgctggtag ccgtccttgc ttccattgcc attttcccgg cagttttttc
     3121 ttttggtatc aatccagcgc aaggccccga actggtattt ataacgctgc ccaatatttt
     3181 ttcacggttg cacggaggtt acatctgggc agttatgttc tttgttttac tggctttagc
     3241 ctctttaact tccaccatct cgctgctgga agtaattgtt gcttttctca cagaagaatt
     3301 acatatatca cgagcgaaag ctgcaattgt ttcttcctta ggcattgcat tattgggcat
     3361 actttcatct ttttcgttag ggatatggaa agatttttca ttgtttggat atggtttctt
     3421 tgctctgttc gacagtatta cttccaaaat tctcatgccg gtgggaggta ttttcatttc
     3481 attatttgta ggctggagaa tcgacaagca gaaaatttta ctcgaactca gcagcaacgg
     3541 aaaatacaag ataccttttc tgaatatttt tttgttgata acccgatacc ttgcaccact
     3601 tgccattcta ctcgttctac tgaatcagtt aggattgacc aaatggttgg ggttctaaaa
     3661 aattgagaca ttattccgaa taaaaataac aatgaataca tttgaaaatg accgtgtgag
     3721 catcaaaacg cacgaaaaaa tgattgaagc cagcaacata cacaaaagtt ttggtaactt
     3781 ggaagtgctg aaaggcgtag atttatcggt tgacaaagga gaagtggtgg ccatagtggg
     3841 accaagtggt gcgggaaaaa ccacactgtt gcaaattttg ggaactatcg acaaaccaaa
     3901 cgacggcgtg gtatccatca acggggtaac tttcagtaaa ttatcagaaa aggaactatc
     3961 ggcttttcgc aatcgaaata tcggttttgt ttttcagttt catcaacttc ttcccgaatt
     4021 cacggctttg gaaaatgtaa tgtttcccgc cttaattgcc ggaaaagacg taaaaaaaac
     4081 caccaataaa gcaaaaaaaa ttttggaaga acttcagctt gcagatcgaa tgcaacacaa
     4141 gcctaacgaa ttgtcgggtg gcgaaaaaca acgcgttgcc gttgcccgcg ctttaatcaa
     4201 tgatcccttg ctgattctgg ccgatgaacc ttcaggtagc ctcgactcca aaaacaaaac
     4261 ggaacttcac aaattattat ttcaactacg cgacaaatta ggattgacca ttgtcattgt
     4321 tacacacgat atggaactgg ctttacaaac cgacagggta atagagctga aggatggaat
     4381 ggtactgaaa actatagaaa aagaaaaatt tgtagtctcg ccgggaatcg aacccgaatc
     4441 taaagtttag gaaactttcg ttctatccat tgaactacaa gaccattttt ttgaaagtag
     4501 tgcaaaatta cattttttcg gcgataaata aaaatttact ttccccttct ctatctttca
     4561 catcctgaaa actatcaacc gtatgagcaa aaacaaattt tttgttgtct ggaaaggaaa
     4621 acagcccggt atttattctt catgggaaga atgcaaacgg caagttcacc aatttgaagg
     4681 cgccctattc aaaggttttg ctactgaaga agaagcaaaa aaagcattcg aatcccttcc
     4741 ttggaaatat atcgacatac gaaataatgc gcaaaacaaa tcttcaggga agaaagggaa
     4801 gaaaacatct atcgggcaac cgataatgga aagtatttcg gtagatgcag cctgcagcgg
     4861 caatccggga ctgctcgaat atcgcggagt ttataccaaa acaggcaagg aaatttttca
     4921 tcagggccca tttcggaacg gaacaaacaa tattggcgaa tttttggcta ttgtccacgc
     4981 actggctcta atgaaacaaa acaacagtca gttgccactc tactccgaca gccgcaccgc
     5041 actgaaatgg gtacagcaaa aaaaggcaaa gaccaaactt gaaaagaatg aagaaaacaa
     5101 atctttgttt gaactgatag aacgtgcaga aaactggctg caaaacaatg attattctac
     5161 tccgctgctg aaatgggaaa ctgaagcttg gggcgaaatt ccggccgatt tcgggagaaa
     5221 ataacttact ttgtctcctt tccaaaaggt cgaggtttga gcaataatcc ggtgaataaa
     5281 gtaaaatagg ttattccttt ttgcaaggaa agcggcattt ctatttgcat cattacaaaa
     5341 taaatcaaca gaaaatagag cagcaaaaaa tttttatcct tgcaggcaaa ataaaacgaa
     5401 cttcccatta aagacagtaa caagagcaag ccgggaatgc ccaaatgaaa caagtctcct
     5461 aaaaattgat tgtgagctgt tgggaaccaa ggaattagga ctttcattcc accggtaccg
     5521 gtacccaaca aataatgatc ttttaaaaac gaaagtacaa tgtcggtttg aatttcgcga
     5581 ggacggtcgt aaaaataacc gcgataattt ttataaatat aaaatccggc tgacaaaact
     5641 aaaaaagctg aaaaaacaat tactgaaaga acaagctttt tcttttttcg caggttgtaa
     5701 agcaaggtta aaaaaatacc aaacgggaac ataatcattc caatacgact ttgcaacaat
     5761 aaaatgacta cggctgtcat gaacccataa aaacccattt cgaaaccgga tattttaact
     5821 gtttgcggtg aattgtcgtt tcgccacaaa taaaacccga ttccaaacat cagcgtcaaa
     5881 atgaatgaaa cgaaagtcgg atgattgtag ccggcccaac ccaacaacaa atagtaatcc
     5941 actccgggag gtacaggtgt gcagaaataa ttctttttca gatgaaatcc aactccaaaa
     6001 tcaactttat gaaattcaac ctgataaaaa taagccacta ttaccattaa taaataaact
     6061 tccaaaaacc gaaagaaaat acgcagcaac aaatttttct tttcctcgga gacagaataa
     6121 aaaccaaaag ccaaaggtaa aacaataaaa gacagccctc ttcccacaaa acgccaacca
     6181 taagcagtat caatcgacca cagaagccca acatattgaa acaaataata ggctcccata
     6241 aaataaaaaa gcggatgagg ttgaaagtgc ttctttttta ctatcgcaaa cagcactgcc
     6301 aaaacaacaa aaaggacggt ggccgtaatc gaaacattaa cacttaaaac cgtagaaatc
     6361 aggagaaaaa acaacgaaac ggtcagaatg ttgtcccatg aaaagttcca ttttggtaaa
     6421 tgacttttaa aaatgacaag aaaaagtatc agttgaacaa aaaaataact cccggccaat
     6481 aatgttaaaa caggaatatt ttgctcaatg ccaatagccg tatatcgata aattaagccg
     6541 gtgagggcta taaatacaaa aaaaacaatt atcgacaaaa gaacaaaacg agttcccaaa
     6601 gaaagttttt tattcattct aatgtctaat tttttggttg taacgagctg atatttttat
     6661 gttttttcca acgataccgt tgaacgagtt ctacacattc ctgcttttca aaaccacgtg
     6721 ccacagcgta ttcttcagcc attgttttca aagcttcggg ctccagattc aatctttcaa
     6781 agtttctaca acctgcagtt ttggatattt ttccaaattt caaccgattc aaatcaatca
     6841 attcaatgtg aatatcgtca ttcgtttgag aaaacaaaat gttccctgcc gaataatccg
     6901 aatgtaaaac tcctttttcg tgtagctcag ccgtaaaacg tgcaacagct ttcaatatct
     6961 tttctcggtc gggaaaattc acgtctcgaa tcaattcttc aaaacgatga ttgcattcag
     7021 agctctccga cacataaaaa cttttattga atagaaaaaa aatcttttgc tcataatagc
     7081 caatcggttg tggagtggca attccaagcg agagaagttt caatgcatat tcatacgaac
     7141 gtctcgcttt cgattttctg aaaataccat atataaagcg attcaccaca tttggcgatt
     7201 gaaacgattt cacaacccat ttcctgtttc ccgcattaaa aattttaatg gtatttcttc
     7261 ccgaatgaat cgtttctcct tgattgtcaa aaatatccgg caacgaataa aaaaattctt
     7321 cttcctcttt gaaatcggga tgaacgaaaa aagtttcggg cttgtaacaa tgataaaaaa
     7381 aattcaacaa gcgagatagt gagcgtctat aatgaagttt tccacctaat tcgcgataaa
     7441 aaaaagcttt atgtcttcgg tttaaaacgt cgataatgtg ttttttcagt tttctgtcgg
     7501 taattctttt agaattactc ctgattcgat agtaaaatcc tgtttcagaa agatgctcca
     7561 cttctcctcc atttttcagc aacgatatcc aaaattccca gtcttcacga ccaataattt
     7621 cctcacaata accgcccact ttcaaagcat ctctcctgcg atacatcgaa caattattaa
     7681 taacattctt ccgagcaatc aattttctgt gataaggtcg atatttccct ttaccctgtt
     7741 tctcgccaaa atattcatat tcacacgtaa caatctttac ttgaggcaat tgaacaagta
     7801 ctttagcagc agtttcaaga aaaatttcag aaatcttatc atcggcatct accggcaaaa
     7861 caaattctcc acgagcttcc tgcaaggcaa gattacgggc tgctgccact ccacgattgg
     7921 attgacggta gaatcggatt cgtgtatccc tttcactgta atgttctgct atcactggcg
     7981 actggtctgt agaaccatcg tcaacaacaa ttatttcaaa atttggataa gttgaagcca
     8041 gtacagaatc tatcgcttct gccagaaacg cttccatatt gtacaccgga ataatgacag
     8101 agaccaaagg attttccatt ttctcaaatt ttatctctac cttttgtata tttcagccag
     8161 taatatttta aaggatgcgt gtatttaagt tgcttccacg gacgactgct gtgaggtaaa
     8221 tgcaaaacag gcaaataggt aagtaaataa ttgttgaatc ccaattctac agctttgtgc
     8281 gaaataaaca catcatacca tgacttttcg ggattcaaat ttttgaaatc gaatgcttct
     8341 aaaaacgaat tggagagcaa tgtacagcaa aaactcaaac gtttggaagt ctgcaccgtt
     8401 ccactgtcaa aatttttggc ataaagatac ggaaaattga tatttccctc ctcatcgtgc
     8461 gttacagcag caatcagacc cggattttca agtttctgag cagcatcaaa caaaatttga
     8521 atggtgtttt ttttcactat tacatccgac tccacaacca gcaaatgtgc atgctccgac
     8581 aaagcctctt gttgggacag ttgcaacacc agcaaatagt ttggagaggg atgcgtggta
     8641 atatctttca aatttaccaa cctgaatccc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn agcatcatca caaaataaca ggcaaacgaa gcccacgccg aagccatata
     8821 actgaaaacg ggaacaaaaa taatattgat ggcaattgta atcaaacttc ccacaatcga
     8881 aatccaagct ccatacatgg ttttgtcggt aagtttgtac caaagcgaaa gattaaaaaa
     8941 cacaccctga aacaaatagg aaaacaatac gatgggaaca acacgcaacc ctgcccagta
     9001 atcgcttcgg ataatgaatt tgaaaatatc cagataaaaa accataccca aaaaaatcat
     9061 caaggaaaaa atgatgaaat atttcatcgc gtcggcatag gcagctgtac tgttcttatc
     9121 cttatattgc gcgaaaatga aaggttcata agcataacga aaagcctgag taaacatcat
     9181 catcaccatc gaaattttga aacaggcgcc gtaaattccc aattgagcag ctgcataggc
     9241 tttgtcttcg aaaagaaagg gaaaaataat tttgtcgatg gtttgatcga taattcctgc
     9301 tattcccaat aagagtaagg gcaatgaata ttgaagcatt ttcttcaaca aggccatgtc
     9361 caaacggaat ttagcttcca acatattggg cagcaaagcc agtgttacag cagcagtcga
     9421 aatcagattg gctacaaaaa tataacccac tccgtaatgc ggattgtaaa accaactgat
     9481 taaaccgggc gctttctttt gcaaccacgg acaaataacc agaaaaaaga tattgaaaaa
     9541 aatgttggta aaaatcatga aaagctttaa tgcagcaaat tttatgggac gatttttata
     9601 gcgcaaataa gcaaacggaa tggcatcgaa agcatccata gaaaccacta tggccagcat
     9661 agctataaat tcgggatatt taggataacc cattccattg gcaatgggtt gcgaaaaaat
     9721 cacacaaatg gttgcaaaaa gtaaggaagt gaaccccacc gaaatcatgg tggtaccata
     9781 aactttaggt gcatcatcca gatttttatt ggcaaaacgg aaaaatcccg tttccattcc
     9841 ataagtaaga atcaccaaca acagagccgt ccaagcatac aggttggtaa ctattccata
     9901 atcagccgaa ctttccaaca cgtaggtgta caagggcacc aaacaccagt tgagaaattt
     9961 ccctatgatg ctgctcaatc cgtaaatggc tgtatctttt gccagccttt tcatctgctt
    10021 ttccggcata ttctctttta aatatatttt acaatggctc ggttgaaact gctaaaattt
    10081 ttcaaaaaag tgtatccatc atccccattt tcgactttcc caagtgcctg tatgccaact
    10141 ctgtaacttc tcttcctcgt ggcgtgcgtt tcagaaagcc ttccatgatc agaaatggtt
    10201 catatacttc ttccaacgtg ccggcatcct cgccaagtgc tgtagcaata gtagacaacc
    10261 caacagggcc accattaaat ttgtcgataa tggtagttaa aattttattg tctatttcgt
    10321 ccaatccata tttgtcgata ttcagagctt cgagcgcata acaggcaatt tccaaatcga
    10381 ttttgccgct tcccttaacc tgtgcaaaat cgcgtacacg ccgcagcaaa gcatttgcca
    10441 cgcgaggagt tccccgactt cgactgccaa tttcaatggc agccctctca tcaatttgca
    10501 cattcaacaa ataagcagaa cgtttaatga tatgcgtaat cacttctgta tcgtaatact
    10561 cgaaatgaca attaatacca aatctggctc ttaagggaga agtcaataat ccgctgcgag
    10621 tagttgctcc aatcagtgta aaaggattca gctccaactg aatggaacgc gcacttggac
    10681 ctttgtcgat cataatatct atccggtaat cttccatagc cgaatataaa tactcttcca
    10741 ctaccggact cagtctgtga atttcgtcaa taaacaaaat atcgttcggt tccaggctgg
    10801 tgagcaaacc ggccaaatcg ccaggtttat cgagtacagg ccccgaagta attttaatgc
    10861 caacattcag ttcgtgagca atgatattgg acaaagtggt tttccccaat cccggaggcc
    10921 catgcaaaag cacgtgatca agcgattcgg aacgcatgcg agccgcttga acaaaaactt
    10981 tcagattttc tacaatttta ttttgtcctt caaaatctga aaacgtcagc ggacgcaatg
    11041 cattttcaaa atccttttcg atattgttgc tccttatgtc aaaattctct gtcatgtaaa
    11101 actccaacaa aaaaaattgt atcctgaatt atttccgtgc caaaattttt ttccaacatt
    11161 aactttctgc ggtcaaactg cgaaagacct gctcaatttc ctgttccacc aatttcatca
    11221 gcagaatttt atttccctgt ttcacggaaa aatcaaaaat ctcactgcga atatcttccg
    11281 aagccacaat caacagacgg gtttcatcca aaaaattcac tttcaatacc ccaggaatat
    11341 tttcaatatc ttttgctgcc ggttttttaa aaaattctac ttcaacctga aaaatatccc
    11401 gatttaaaga gtgaatggac gaaagttcct caaaatctgc agcaattttt cctttattta
    11461 taatgattac tctgttgcac aaagctttaa cttcctgcat aatgtgagta ctgagtaaca
    11521 cagttcggtc ttttcccaac tccagaatta aagttctaat ttcttccaac tgattgggat
    11581 ccaagccggt tgtaggttca tccaaaatca aaactttcgg ctcatgaatc aaagcctgtg
    11641 ccaacccaac ccgctgacga tatcctttgg aaagctgtcc gatttttttt cggaattcgg
    11701 cacgaagacc taccttatca atcatctcat ccactttttt gttcctgtat tgtcccaatt
    11761 tgtaaacatc cgcaataaaa agcagatatt cttttacata catttcctcg tacaatggat
    11821 tttgttcggg caaataacca atcagcgatt tgattttcaa aggcgcgtcc ttcacttcat
    11881 gaccacaaac ttttacactg cccgtttcgt aaggcaaagc tcctgcaata attctcatcg
    11941 tggttgtttt tccggcgcca tttggcccca aaaaaccaac tactccattt ttgtcgatgt
    12001 caaaacagac atcgtccaaa accttttgtt gtccgaagtt ttttgaaagt tttgttatat
    12061 tgatggacac acgtttcgtg ttttgtaaaa agttatattc aaaaatttcg actgcaaaag
    12121 taataaatcc ttaccgtata acgaaatgaa agagaaagcg ttttaaacaa gaaaaaagtc
    12181 aacttcctat tcggacagaa cgaaacggca gaaaaaatca aattttgaaa acaactttaa
    12241 caactgccaa aaaaagatag gcaataataa tgggaacaaa agcattcagt tgaaaaacaa
    12301 aaagaaacaa aacacacaac atcaagaaaa tatattgcaa ttcattttct tcccatttca
    12361 ggtttttgaa ttttaaagaa aacatgggga tttccgatac cagcaaagca ctgaaaatta
    12421 caatcaaaat caaaatcaac caactgtttg ccgttaagaa ttcggaaaaa gaatagccca
    12481 atccaaccca aaaaatggca tttgccggag ttgccaaacc caggaatgag gaaccttgcc
    12541 gctcatcgat gttgaatttg gccagtcgca gagccgaaaa caaagtaatg acaaatccgg
    12601 caaacgctac ccaaaacggc aaggagcttt gagagagcaa actaaaacat accgctccgg
    12661 gtgccaaacc aaaactcaca acatcggcca aagaatcgag ctcttttccc ataggcgaat
    12721 aagcctgcaa caggcgagca gccaatccat caataaaatc gaaaaaagca gcaatcaaaa
    12781 tgaaaaccaa aacagcaaaa taatttccct gaaaagcata ataaacccca atacaaccag
    12841 aaaataaatt caagcaggtc agaaaatttg gaatatgttt tttcattctt tatcctaatt
    12901 tatggtaaat acttttttaa aaccactgaa atcaaaaaaa tcgaaaactt tggaacaaca
    12961 aaaaaatggg acaaacattt tagtttttga ttttttctgc aatgcaacag caaaattatc
    13021 aaactttact gtctatccaa atagttaccg gaccatcatt taccagttta actttcatat
    13081 cggctgcaaa aacgccggtt ttaacctctt taccggtttt ttgttccatt attttacaaa
    13141 aactttcgta aagcggaata gctgtttcat gccgtgcagc ccgaatataa gaaggtcggt
    13201 tccccttccg agtttctgca caaagtgtaa actgactcac caccaacact tctccatcga
    13261 tatccgtaac ggaaagattc ataactcctt tttcgtcgtc aaaaatacgt aatgctgccg
    13321 ttttaccggc caaccattcg gcatcctgca aagtatcggt gctttctata ccaatgaaaa
    13381 ccagcaatcc ttttcctatg gaagatatta ttagtcgatc aatttctacc gaagcttctg
    13441 ttactcgctg aactaaaacg cgcatgacaa attttgcttt ttaacgcttt ttgaagcaaa
    13501 atagattaaa ctcaaacatt gaatcggaaa tgcataatat cgccatcctg caccacataa
    13561 tccctgcctt cgatataaat ctttcctgct tctctgcaag ccgtttccga gcctaaggcc
    13621 acaaaatcct catacttgat tacttcggca cgaataaagc ctttttcaaa atcggtatga
    13681 attacgccgg cacattgcgg agctttcaat cctttttcgt aagtccatgc ccgaacttcc
    13741 agtggcccga cagtaaaaaa tgtttccaaa ttgagcaaac gataagccga ttgaatcaag
    13801 cgggatactc ccgattcctt taaaccaagt tcctccaaaa acatctgacg ctctttatac
    13861 gattccagct cggcaatttc ggcttcagtt gcggcagcca ccaccaatat ttcggcattc
    13921 tcttccttta ctgcttctct caccgcctcc acataagcat ttccgcttgt tgcagaagct
    13981 tcgtccacat tacagatata cattacaggc ttgtcggtca ataaaaaaag atctttgacc
    14041 aactttttag attctttgtc caacactacg gtacgtgccg attttccctg taacaaagct
    14101 gaccgatatt cctgcaaaat ttcataaatt attttagcct gcttgtcgcc accggtttgg
    14161 gcctgtttct gcactttatt tattcgactt tctactgttt ccaaatcttt caactgcaac
    14221 tccgcatcca ctatttcctt gtcacgaacc gggtcaataa ctccattgac gtgggccaca
    14281 ttttcatctt caaaacaccg caatacatgc agaatggcat ctgtttcacg aatattggcc
    14341 aaaaatttgt ttcccaagcc ctctccttta ctcgcacctt ttaccagtcc ggcaatatcc
    14401 acaatttcta ccgtagccgg gactaatctt tggggatttg ccaatcgggc caagatatta
    14461 atcctttcgt cgggaacttg cgtaacgcca acattgggtt caatggtaca aaaaggaaaa
    14521 ttggccgctt gagcctttgc attcgacaga cagttgaata atgtggattt gccaacattc
    14581 ggcaaaccta caataccaca ttgaagtgcc atataattat ccgatcaatt aaattattct
    14641 tcttttttaa aactatttct ttgtacaaaa gtacatgata attttcaatt tacacaccga
    14701 ctatttaaaa acaaactttc ccgattcata aaatcaaacc tataaaactc ctttcagtaa
    14761 tcatgcaaat gaatgaaaat gaaaaataag ctgaaacttc ttcaggaacg atctgaaaat
    14821 tgaacggaga aatttatttc catatttcgg caatgattcg gggaaaaaac tcgtattcga
    14881 gagcatgtac cctttgagca accgattcgg gagtatcgtc gggcaacacg ggacattttg
    14941 cctgaaaaat aatatcgcct tcgtcgtaat ttgaattgac atagtgaatg gtaatgcccg
    15001 tctccttttc tccagcttcc aacacggcgc gatgcacctt catgccatac atacctttcc
    15061 ctccgaactt tggcaacaaa gcaggatgaa tattgacaat cttttgcgga taagcttcga
    15121 taatcgaatc gggaattttc agtaaaaatc cggacaaaac aatcgcatca atatttttct
    15181 cctgaagtaa attcaaaatt tttcctccat cgataaaatc ttcccgcgga aaagtaagcg
    15241 aaggaacacc cagttttttt gcccgctcat gaacaaaagc tttctcgttg ttggaaataa
    15301 tgagaggaaa agaaaaatcg gaattgcctg taaaataacg aattatattc tcagcattgg
    15361 aacctgaacc cgaagcaaac aaggcgatat tttttgacat aattgatgaa gaaaaaatgc
    15421 tcaaaaacag gtgatttgtg caaaaaaaca tatttttttc taaaaagtga tgattatttg
    15481 aagaaaattt catttctttg caccgcaaaa ttataaaaca atttttattt ataaacttaa
    15541 aacttaaaat tatgtcagaa gttgaagaaa aagtaaagac tattattgca gacaaattgg
    15601 gtgttgatga agcagaaatt acaccatcgg caagctttac caacgatctt ggcgctgatt
    15661 cgcttgatac ggttgaattg attatggaat ttgaaaaaga attcggtatt tctattcctg
    15721 acgatcaagc tgaaaaaatc accactgttg gcgatgctgt aagctatatc gaaaatgcta
    15781 aaaaataaca ttccttattt gttatggaat taaaaagagt agttgttaca gggctgggag
    15841 ccattactcc attgggttca tctgttccca aactgtggga aaatatcctc aatggggtaa
    15901 gtggagctgg tcccatcact cacttcgatg cttctcaatt taaaacgcaa tttgcttgcg
    15961 aagtcaagga ttttgatcct tttcagtatt tcgaaaaaaa ggaaatcaga aaagtcgatc
    16021 cttatgctca gtatgctatc gcagctgcca agcaagctat tgaaaactgc ggaataaatt
    16081 tggaaactgt cgacaaggat gaagttggcg tcatcttttc gtcgggaatt ggcggaatca
    16141 ctacttttga gcaggaagtt tcaaactatt ttctcaacaa ggaaatgggc ccgaaattta
    16201 acccgttttt tatcccgaag atgatttcag atattgcagc aggtcagatt tccattcttt
    16261 atggttttaa aggcccaaat tatgctacaa cctcagcttg tgcttcatct actcattcaa
    16321 ttatcgatgc cttcaattac atccgtttag gcaaagcaaa aatgtttgtt gttggaggag
    16381 ccgaagcagc catcacacct gccggagttg gcggattcaa ttccatgatg gcactatcta
    16441 cccgaaatga tgatcctgca ggagcttcac gccctttcag caaaagcaga gatggttttg
    16501 tcattggcga aggagccggt tgcatgattc tggaagaatt ggatcatgct ttggctcgtg
    16561 gagcaaatat actttgtgag gtggcaggcg gcggcatgtc ggctgatgct taccatttga
    16621 cagcttcaca ccctgaagga cttggcgcta tcctcgccat gaagagtgca ctcaaagatg
    16681 caggaatgca agttgaagac atcgactaca ttaatgttca tggtacttct acgcccgttg
    16741 gcgacacatc cgaagcaaaa gccattgaaa acgtttttgg agaacaggca tatcaactga
    16801 atatcagttc aaccaaatcg atgacaggcc atttgctggg cgctgccggt gctgttgaag
    16861 ccattatctg tacgcttgcc attattaatg atgtggttcc gcccaccatt aatttcacgg
    16921 aaggcgatga agaccccgat attaattacc ggttaaactt taccttcaat aagccacaga
    16981 aaagaaccat taatgcagct ttatccaaca actttggttt tggtggacat aatgcaagtg
    17041 ttatattaaa aaaattcacc aaataagttc gtgaaaaatt tctttcattt gaatgggctc
    17101 ttttccaaat acagaaaaga gccttattct ttgttccgaa aactattggg cttttatccg
    17161 cgcgatatca actattacct gctggctctg acacatcgct ccttgtcaat gacgaaccat
    17221 gatgggaaag ttgtgagcaa cgaaagactt gagtttttgg gtgatgccgt attgaacgct
    17281 atcgtttccg atttatttta ccggtattat gagcaaaaag atgaggggtt tctcaccaat
    17341 attcgttcca aaattgtcaa tcgcgattcg ttgaatcact tggcactgaa acttgaattg
    17401 gacaaaattg tgaaagtttc caaaaatgtg aatccgcaag tcagcaacga tatttacgga
    17461 aatgctttgg aagctttaat tggcgcaatt tatctcgatt acggatacaa ccactgtatg
    17521 acgtttgtca aaacaaaact ttttgagcct tttatcgatt tgtcaaaagt agaaaaattc
    17581 gagatgaact ataaatcgaa attaattgaa tggtgtcaga aaaatcactt ccaaatcgac
    17641 ttcgagatgt cctgcaaaac tcacaacgaa aaccatcaac caatttttct gtcgcgtgta
    17701 ctcatagaag gaatgcctgt ttccgaatca acgggactga acaagaaaga atctcaacaa
    17761 aaagcctctc gcatcgccta tcaacaaatt atcgagcaga aaacatttca ttttataaac
    17821 gaagaatcaa aaaattagtc tgcctcaact cagcaaaact taagttgaaa aagctactaa
    17881 aaggaaattc cttttcagca acagattaaa ttaatttttt ggacaattga aaacttttaa
    17941 ctaactttgt aggtgatata aattttttgt ataactaaaa accaaaccgc tatgtcaaca
    18001 gaaaacatcg gaattaatgc cggagccgta tggaaagtat tgaacgaaaa aggagaaatg
    18061 gacgttaaag cgcttaaaaa aagcaccaaa ctctctgaaa aagatttgaa tatggcacta
    18121 ggctggcttg cccgagaagg aaaaatcgaa tttaacgaaa cagaggaaaa tttattggtt
    18181 cacctcgttt gagagagtga aatataaaaa aggcggaatg gaaatttcgc cttttttcaa
    18241 attttaagga atcctgcttc acttttcaag ccaaaaaatt tcgtaaagcg aaaaaatgct
    18301 tgtatatttg cacccgcttt acgcatgaaa atgttagata tgcgaaaata gctcagttgg
    18361 tagagcacga ccttgccaag gtcggggtcg cgggttcgag tcccgttttt cgctcattga
    18421 aaaagtcttt tacttttttg cccggatggt ggaatggtag acacgaagga cttaaaatcc
    18481 tttggcccca aaggctgtgc gggttcaagt cccgcttcgg gtaccaacaa accccttact
    18541 catctttaag atttgcaagg ggtttgttat ttttttgcaa attcctaatg aatttgcttg
    18601 ggctcacctg caaaaaacgg ctcacctgca aaaacatgtg t
//
LOCUS       DAOSIB010000040        18455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_3238_length_18455_cov_12.624239, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000040 DAOSIB010000000
VERSION     DAOSIB010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18455
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3238_length_18455_cov_12.624239"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            387..1706
                     /locus_tag="PLD44_05095"
     CDS             387..1706
                     /locus_tag="PLD44_05095"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOF98742.1"
                     /translation="MKVLKFGGTSVGSAQRMKNVAKLICNGEKKIVVLSAMSGTTNSL
                     LEIADYFYKSNLDGALEHIITLENKYSDVVEELLTTEEKRQEARSILSSHFDYLRSFA
                     KSVFTIFEEKAIVAQGELLSTALFQLYLEEIGENSILLPALEFMRIDKNAEPDNAYIK
                     QHLNDLLEAHPGHEIYLTQGFICRNAYGEIDNLQRGGSDYTASLIGAAVNAEEIQIWT
                     DIDGMHNNDPRFVESTKPIPMLHFEEAAELAYFGAKILHPTCILPAKLANIPVRLLNT
                     MEPEAAGTLISTRMNKGKIEAVAAKDGITAIKIQSGRMLLAYGFLRKVFEIFESYKTP
                     IDMITTSEVGVSVTIDNTKKLDEIVNDLKKLGTVTVDHDMVIVCVVGDLPFENVGFQA
                     KVVDALKDIPLRMISYGGSNYNISFLIKAEDKKRALNALSQALFSEE"
     gene            1756..2919
                     /gene="lysA"
                     /locus_tag="PLD44_05100"
     CDS             1756..2919
                     /gene="lysA"
                     /locus_tag="PLD44_05100"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOF98743.1"
                     /translation="MKAKYPVEKFLKLQTPFYYYDMDILRQTLQEVNRQIQKRNYVVH
                     YAVKANANPVLLHEIKNAGLGADCVSGGEINAAIKAGFPKEKIVFAGVGKADWEINLA
                     LDQNIFCFNVESIPEMEVINQLAAAKSKTASIALRINPNVNAHTHQYITTGLNENKFG
                     INLSDLDTVLDLLPQLKNLKLIGIHAHIGSQITDMTSFQSLCVRINEIQNYFFQRHII
                     LEHINVGGGLGINYEHPNHFPIPDFESYFNVFAEHLNLQPYQTLHFELGRAIVAHCGS
                     LISRVLYVKQGATKKFVILDAGFTDLIRPALYQAYHRIENLTSDLPEEEYDVVGPICE
                     SSDTFAKNYSLNGTRRGDLVALRSAGAYGEIMASQYNLRSLPKAYTSDEIAAL"
     gene            3032..4195
                     /locus_tag="PLD44_05105"
     CDS             3032..4195
                     /locus_tag="PLD44_05105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOF98744.1"
                     /translation="MTEIFGFSLSLLTLVLLCGLFLVFCYQIYFYCRYINGVIRYRNK
                     IRKGKVAFLDEKPGVSVIICSKNEANKLKEFLPSILEQDYPDFEVIVVNDGSTDETDM
                     LLIDLLKKYPRLRTTFVPMEATNLSTKKLGISLGIKAAKNDFLLFTDADCQPKSKDWI
                     SLMARNFVPKVEFVLGYGAYFHRKGLLNRLINYDTFFIALQYMGMAIAGKPYMGVGRN
                     LAYRKTTFLHHNGFSEILGLISGDDDLFVNKTANGNNTRVEISPESITFSEPKTTWKS
                     WYLQKARHLSVSPFYTWQSKLMLFMEPFTRGLFYVFCLVLLLQGDMIISLVVAAIFLI
                     RYFTQLYIVNRTAKHFNERRFFLLLPIFDLVLPILSLSIFITGKASRRKNKKW"
     gene            complement(4217..4750)
                     /locus_tag="PLD44_05110"
     CDS             complement(4217..4750)
                     /locus_tag="PLD44_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009229196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOF98745.1"
                     /translation="MRIISGRLKGRNIYAPVDISARPTTDFAKEGLFDVLNNRIDFED
                     IEVLDLFAGTGSIGFEFVSRGCRQVVSIENNPKQCLFIKKISEKFEIDNLILLKTDVF
                     KYITHTASRFDLIFADPPYQLENLKEIPDMIFSQQLLKTEGIFILEHSSKYNFSQHPH
                     FMETRKYGRVHFTFFKA"
     gene            complement(4761..5504)
                     /locus_tag="PLD44_05115"
     CDS             complement(4761..5504)
                     /locus_tag="PLD44_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOF98746.1"
                     /translation="MYSSRGDLESTNTYIQPYSLSIRFSPGGLSLSILERDNTKLVSA
                     KRLNMPLFSMTKEQIIPLLLQEPETQLLYDKIHLIAETKTYTIVPKEIFKEEEMDKLL
                     YFSHQKEKNASILYNEISAFDAINIFSLPEDISEVFKQLFPACKIEHSISHFIENHIR
                     KHSLQECICIWVRQKIMDVVAFQHNRLLLVNSFDYQTQEDFVYYLLRIIEELNLDFQN
                     CKVYLYNMEQHSKLQGFVRKFVKQCETID"
     gene            complement(5537..6217)
                     /locus_tag="PLD44_05120"
     CDS             complement(5537..6217)
                     /locus_tag="PLD44_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98747.1"
                     /translation="MKTVIKILLSLSIVLLSYLCVASIITPIRFERIKNQREKDVIQC
                     LVDIRKAEIEFKDQNKRYTASFDTLFHFLKTAKKKIVMKEGFLTDKQLEAGLTEAKAA
                     EIVRKGNQKEIAEYGLQNFRRDTILTDLLEEVFPNYNRDNIDKMRIIPYSKNEEFTLK
                     VNNNYTNISGIRIPLFEASALYESYLYDLNRQELLNLIDRTLKMGKFPGLKVGSIEEP
                     NNNAGNWE"
     gene            6698..7516
                     /locus_tag="PLD44_05125"
     CDS             6698..7516
                     /locus_tag="PLD44_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98748.1"
                     /translation="MKKMLCLLLIASTVSQIFPQISSIIPVSSSIAGTSVANTGLRSE
                     FQNPAMLGYAKNSEIGMQHENRYALSELSTNSFQALLSTNAINADLSFSYFGYSLYHE
                     MMTGLGFGRNFSDKFALGVRFNYYMAYFSASNSYRGALFPQIGLSYRFSSLFSLGFST
                     FNPFQTNIKTDFTQKRIPSIFSLGTEYYFSPELVWLAQIDREISSNYRLATGFEYQML
                     QQVSLKLGVYDAGYLVPCGGCGLKWNRFLLDINCELHPLLGLNSYAGLRYIFSR"
     gene            7540..9609
                     /locus_tag="PLD44_05130"
     CDS             7540..9609
                     /locus_tag="PLD44_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOF98749.1"
                     /translation="MKRIVVLCCLLFLLINAAYAQDASTTIDQTIADIFEQWSAESEE
                     VPDYQTFYEELEFLAENPINLNHTSREELSKMLFLSDVQVENILAYLYQYGPMENIYE
                     LQLIDGLDMTDIRRMLPFVMVKKEKAVSEKIYRYDVLNYGKNELYMMFNRDAETRQGY
                     QASVSSIGDSTNQGKKYVGSPWYHSAKYRFHFKDRILAGMTFEKDAGESFFGSAHKGY
                     DSYSGYLQINRLGIFKTIVLGDFRANFGQGLVMKSDFSMGKSSYVLNVVSRNEGLKKF
                     SSTDEYNFFRGGGATIRLPRNWETSVFYSNKMIDADTVGGVFSSIYKTGYHRTENELN
                     KKHTICQQVAGGNISWRLLWLQTGITFVHTNFDQALVPEKTTYNRFYFSGKAQNTAGW
                     NYRVHWQKLNLFGEAAVTGNFHPALLSGFTLSPLSLVNLVAVYRYYSPEYDTFYALAL
                     SETSRTNNESGLYLGAEINPAKKWKISAYADSYRFPWPKYGIDAPSAGKDYLFQTDFS
                     AHRRLSMFWRFRHSNKPANISQENSVMPVVKAVKKNSLRYQLMYSQGNFSFRTAAEGN
                     MVQKGENADYTYGLTAYQDLTYEFSSLPLKLDFRYQFFDAVAYENRLYAYEKDVLYAF
                     SIPMYYGIGSRYYLNLKYELNRQFTLWFKIAQTVYADDREEISSGNEKILGNKITDVK
                     FLLRFNF"
     gene            complement(9843..10265)
                     /gene="tsaE"
                     /locus_tag="PLD44_05135"
     CDS             complement(9843..10265)
                     /gene="tsaE"
                     /locus_tag="PLD44_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOF98750.1"
                     /translation="MTEIFDLNNIQQPARKFINLFDRYSVFAFYGSMGAGKTTFIKAV
                     CEILGVKDTVNSPTFSIINEYEAADGRVLYHFDCYRINKLQEAIDLGAEDYFYSGKIC
                     FIEWPEKIEALLPDSTVKVNIQEVENGIREIEMILPTI"
     gene            complement(10323..12161)
                     /gene="uvrC"
                     /locus_tag="PLD44_05140"
     CDS             complement(10323..12161)
                     /gene="uvrC"
                     /locus_tag="PLD44_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOF98751.1"
                     /translation="MNLHPQLKQQIDALPEKPGVYQYFNEKEEIIYVGKAKNLKKRVS
                     SYFTKTHESRKTAVLVRNISSLKYIVVDTEEDALLLENNLIKKFQPRYNVLLKDGKTY
                     PSICITNEEFPRVFKTRNIIKNGSLYFGPYSSSYTINSLLEIIHDLYPIRTCKLQLTD
                     ENIKAGKFKVCLQYHIHKCNGPCEGKESAEIYRKYINEIKQIIEGNANEISKMLWEEM
                     QKLSAAYKFEEAYQIKQKYDLIENYKSKSIIANSVVENTDVFGYEETENAAYINILRI
                     SKGSIIQGYTIEYQKHVDEEKEDLLAMAIVELRERLKSNSKEIVVPFEINYKPNGVSI
                     VIPQRGDRKKLLDLAQQNVLQYKFEKLKQAEKLNPDQRSMQLLKEIQEKLHLEKLPIT
                     IECFDNSNIQGKDAVAACVVFKKGRPSKKDYRKYNIKTVVGPDDYASMKEVVKRRYSR
                     MIAENGELPDLIIADGGIGQMEVIREVVEDELNLRIPIIGLAKDSKHHTREILFGFPP
                     KNIGMTPNEPLFKLLATMQEEVHRFAIKFHREKRSKNQTTSELDNIKGIGDKTKRELI
                     NHFKSIKRLQNANLEDIQKVVGNHRASIVYGYFHKDLSAPESQISK"
     gene            complement(12242..14116)
                     /gene="mnmG"
                     /locus_tag="PLD44_05145"
     CDS             complement(12242..14116)
                     /gene="mnmG"
                     /locus_tag="PLD44_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOF98752.1"
                     /translation="MDFKYDIIVIGGGHAGNEAACAAANMGSKTLLITMDMNKIGQMS
                     CNPAVGGIAKGQIVREIDALGGQMGVVTDRSAIQFRMLNRSKGPAMWSPRSQSDRHQF
                     ILQWIKTLTNTPNLYIWQDTVKQILIENGQVTGVLTALGIEISAKCVVLTAGTFLSGL
                     IHIGKNQLIGGRISEPASFGITEQLKSLGFTTGRMKTGTPARIDGRTIDFSVLKEQPG
                     ENDFHKFSFLTEVTRELKQMSCWITYTNELTHDILRQGLPESPLYNGQIKSIGPRYCP
                     SIETKIVTFADKSSHQLFLEPEGVDSQEYYLNGFSSSLPVQVQIQAIHTIPGLEKAEL
                     YRPGYAIEYDFFDPTQLKHTLETKRIKNLFFAGQVNGTTGYEEAAGQGLIAGINAHIN
                     CHGGKEFLLDRDEAYIGVLIDDLVTKGVDEPYRMFTSRAEYRILLRQDDADLRLTPKG
                     YEIGLVTKNRINQYSFKYEAIESLKTFVENFSIKPAQVNGFLVSKNSSELTHGCKLVD
                     LILRPQISIMELSEIVPALKSKIQSIGDRVEEIVEAAEISLKYKGYIDREKSIAEKFH
                     RLEHINIKGKFDYHTISSLSTEARQKLSKIDPETIGQASRIPGISPSDINILLVLMGR
                     "
     gene            complement(14196..14687)
                     /locus_tag="PLD44_05150"
     CDS             complement(14196..14687)
                     /locus_tag="PLD44_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007748508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOF98753.1"
                     /translation="MSENFIISSSLDKKSIYESLLLQIPALIADEPDIVANLANLSAV
                     FHQTFKWWWVGFYLVKEDFLVLGPFQGPVACTRIAFGKGVCGTAWKEKRSLLVPDVND
                     FSGHIACSSISVSEIVVPLYNSSSEVVGVLDIDSEKFGLLDEADIYYLEQISTIIGKA
                     LFA"
     gene            14905..17727
                     /locus_tag="PLD44_05155"
     CDS             14905..17727
                     /locus_tag="PLD44_05155"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOF98754.1"
                     /translation="MRKLELKFFPFVLLICLNTSIFAQQVQFQPLPIDPKVKYGKLEN
                     GLTYYIRSNQQPKNRADFYIVQNVGAILENDDQNGLAHFLEHMAFNGTKNFPGKKIIE
                     YFESVGVKFGANINAYTSLDETVYHLSNVPTNRESIIDSALLVLHDWSNAISLEENEI
                     EAERGVILEEWRQGASADRRMWKESNKQKFPGSQYAKRDVIGDTAVIKHFTPEALRDY
                     YKKWYRPDQQAIIVVGDVDVNKIEEKIIELFSNIKKPENTPERPIYPIYDNKEPIISI
                     VTDPEARMTRVELIYKHDKLPAEIKLSINGYLVNVINSLISNMMQNRFEEITMQPDAP
                     FVAGYATYGELVKSKDAFYLVVVPKEGSETEGIKSVLIEAEKVKRFGFNHSELERAKA
                     DLAKMIEKQYNEKENQKNESYVREYMNHFLNQNPIPGIEWEYETIKMILPQINADMVN
                     QLAKSYVTDTNMIVSIMAPQKPTVVLPSKEEIRQIIEEVKKTEITAKLDEKIDKPLIE
                     KMPKPGKIKKVSKNDILGTTEWTLSNGAKVILKPTTFKKDEILFNAYSTGGLSKVENI
                     ENLPSAEFATEVVNNNGLSNFNQIELKKLLSGKIASVSPYIDNYEEGFSGNSSVADFE
                     TMLQLLYLHFTALRKDDNAFTALMNNYKAFLANSENNPDKAFSDSVQLMLSNHSPRTV
                     LLNMKTLEKVNQDKALEIFAERFSDPADFTFVFTGNVDPNDKTVVNAINTFIGGIKSK
                     GKKESFTDDNVRLPVGRAINHFTREMQVKKVSNCIVYSGNLNFNMNNRTNMNAIGNIL
                     NIRYLESVREKEGGSYGVGVAGNLRNRPIEQAMLFIQFDTNIEKQNKLMDIIHSEIDT
                     IISKGPKAEDLQKVKETMMKKYEEDLQENKWWQSAIKNYYFNKLNVVNDYLPSVQALT
                     SESIQKTLKTIVEQGNVMEIVMKPKE"
     gene            complement(17820..>18455)
                     /locus_tag="PLD44_05160"
     CDS             complement(17820..>18455)
                     /locus_tag="PLD44_05160"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HOF98755.1"
                     /translation="MTPQTVNAYYNPSTNEICFPAGILQPPFFDVHADDAVNYGAIGV
                     VIGHEMTHGFDDQGCQFDKDGNLKNWWTEEDKKQFDARAKVLADYFDSIEVAPGVHAN
                     GKFTLGENIADHGGLQVSFQAYKNSTKGQPSKILDGFTPEQRFFLSYANVWACNIRPE
                     EILVLTKSDPHALGRWRVNGALPHIQAWYDAFGIKEGDKLYLPVEKRACIW"
BASE COUNT         5934 a         3334 c         3308 g         5879 t
ORIGIN      
        1 ctgaatatta tattcattcg tgtttgtgta tgcaatacat gtgggtttca taattttgtt
       61 ttctttcagg agaattacat cttacaaaga taactttctt ttttaacaaa tcaatttcaa
      121 aaatgtttcc cgcattgaaa tttattttcc tttgcataaa gcacattggt agcttggaag
      181 ttgaatccct gcatacaaaa atgtcagaaa attgtattat ttttgtggaa aatttcagaa
      241 ataaatagga atggcttcat tccattaaaa ataactgatt tttaaaacta ttttaagaac
      301 attaagtagg taagggaaac gaaaaattta tttttttgaa tcatttttcg gctttaccaa
      361 caattacaat ataaaaccag acaattatga aagtattgaa atttggcggg acttcggtag
      421 gttcggcaca gcgcatgaaa aacgttgcaa aactgatttg caatggcgag aaaaaaattg
      481 tggttttgtc agctatgtct ggaaccacca acagcttgct tgaaattgcg gattattttt
      541 acaaaagtaa tcttgacgga gctttggaac atatcattac gctggaaaac aaatacagcg
      601 atgtggtgga agaattgttg acaaccgaag aaaaacggca ggaagcccgt tctattcttt
      661 cgtctcattt cgattatctg cggtcgtttg ccaaatcggt ttttaccatt ttcgaagaaa
      721 aagccattgt agctcaggga gaattgcttt caaccgcact ttttcaactt tatctcgaag
      781 aaattggtga aaattccata cttctgccgg cgctcgaatt tatgcgtatc gacaaaaacg
      841 cagaaccgga caatgcctat atcaaacagc atttgaacga tttgctcgaa gcgcatcccg
      901 gtcatgaaat atatcttacg caaggattta tttgccgcaa tgcttatggc gaaatcgaca
      961 acctgcaaag aggaggaagc gactatacgg cttctttgat tggtgctgct gtaaatgctg
     1021 aagaaattca aatctggaca gatattgatg gcatgcacaa taacgatccg cgttttgtgg
     1081 aaagtaccaa acccattccc atgcttcatt ttgaagaagc agccgaattg gcttactttg
     1141 gtgcaaaaat tcttcatcct acctgtattt tgcctgctaa gttggctaat attcccgttc
     1201 ggttgcttaa tacaatggaa cctgaagctg ccggaacgct tatttctaca cggatgaata
     1261 agggaaaaat agaagctgtg gctgcaaagg atggaattac ggcaataaaa attcagtcgg
     1321 gacgaatgct gttggcttat ggttttcttc gcaaggtgtt tgaaattttt gaatcctata
     1381 aaacgcctat cgatatgatt actacatcgg aagtgggcgt ttcggttaca attgacaaca
     1441 cgaaaaaatt ggacgaaatt gtcaatgatt tgaaaaaact tggcaccgta actgttgatc
     1501 acgatatggt tatcgtttgt gtagtaggcg atcttccttt cgaaaatgtc ggctttcagg
     1561 caaaggttgt tgatgcattg aaagatatac ctcttcgaat gatttcgtat ggcggcagca
     1621 attacaatat ttcatttttg ataaaagctg aagacaaaaa acgtgcttta aacgctttga
     1681 gtcaggctct tttcagtgaa gaataacaat tcccgaaacc gaaaagagaa cgttttttat
     1741 atttttattt gtatgataaa agcaaaatat cctgttgaaa aatttttaaa actgcaaacg
     1801 ccgttttatt attacgacat ggacatcctg cggcaaacgc ttcaggaagt gaatcgacag
     1861 attcagaaaa gaaactatgt ggttcattat gcggtgaaag ccaatgcaaa tcccgtattg
     1921 cttcatgaaa taaaaaatgc cggtttgggg gccgattgtg tgagcggagg tgaaataaat
     1981 gctgccatta aagcaggatt tccgaaagaa aaaatagttt ttgccggagt tggaaaagcc
     2041 gactgggaga tcaatcttgc gctggatcaa aacatttttt gttttaatgt tgagtcgatt
     2101 cccgaaatgg aagttattaa tcaattggct gccgccaaaa gtaaaacagc ttctattgcc
     2161 ttgcgtatca atcccaatgt aaatgcccat actcaccaat atattacgac cggcctcaac
     2221 gaaaacaaat ttggaattaa cctttccgat ttggatacgg ttttggactt gctcccacag
     2281 ttgaaaaatt taaaattaat cggtattcat gcccatattg gctcacaaat caccgatatg
     2341 acatcctttc agagtttgtg cgtgagaatc aacgaaattc agaattattt ttttcagcgc
     2401 cacatcattt tggagcatat taatgtaggt ggaggtctgg gtattaatta cgaacatccc
     2461 aatcattttc cgattcccga ttttgagagt tatttcaatg tatttgccga acatttgaat
     2521 cttcagccgt atcaaacact acatttcgag ctgggacgtg ccattgtagc gcattgcggg
     2581 agtttgattt cgagggtgct ttatgtcaaa cagggagcta ccaaaaaatt tgtcatcttg
     2641 gatgccggtt tcacggatct tatacgtccg gctttgtatc aggcttatca tcgtatcgaa
     2701 aatttgactt ccgatttgcc ggaagaagaa tatgacgtgg tcgggccaat ttgtgaatct
     2761 tccgatactt ttgccaagaa ttattctttg aatggaacac gcagaggcga tttggttgcg
     2821 ttgcgttctg ccggcgccta tggtgaaata atggcttcac agtataattt acgctctttg
     2881 ccgaaagctt atacatcgga tgaaattgct gcattgtaaa aacagccgat ttgatgtttt
     2941 tcttttttaa agaaaaattt tagtgaaaaa aaccatttac tgaactttag gaaaaaatcg
     3001 gcaatcaatt ttgaaaaata cgcttaccca aatgacagaa atattcggat tttcactttc
     3061 tttgttgaca cttgttttat tgtgcggact gtttttggtt ttttgttatc agatctattt
     3121 ttattgtcgt tatatcaatg gggtgattcg ttaccggaat aaaatcagaa aggggaaagt
     3181 tgcctttctg gacgaaaaac ccggtgtctc cgttattatt tgttcaaaaa acgaagccaa
     3241 caaactgaaa gaatttctgc cgtccattct tgaacaggat tatcccgatt ttgaggtgat
     3301 tgtggtgaac gatggctcaa ccgacgaaac cgacatgttg ttgatcgatt tgttgaaaaa
     3361 atatcctcgt ttgcggacaa cttttgtgcc aatggaagca accaatttga gtacgaaaaa
     3421 attagggata tccttgggca tcaaggcagc aaaaaatgat tttcttcttt ttaccgatgc
     3481 cgattgtcag cctaaaagta aagactggat ttcgttaatg gcaagaaatt ttgttccaaa
     3541 agttgagttt gttctcggat atggagctta ttttcaccga aaaggcttgt tgaacaggct
     3601 tataaattac gatacttttt tcattgcctt gcagtacatg ggaatggcca tagcaggtaa
     3661 accttacatg ggtgtgggaa gaaatttggc ttatcgtaaa accacttttt tacaccacaa
     3721 tggtttttcg gagattttag gattaatttc gggggatgat gatttatttg tgaacaaaac
     3781 tgccaatggg aacaatacac gggttgaaat ttcgcccgaa agcatcactt tttccgaacc
     3841 aaaaaccaca tggaaaagct ggtatttgca gaaagcccga catttgtccg tttcgccttt
     3901 ctatacttgg caaagtaaac tgatgctttt tatggaacct ttcacacgcg gcttgtttta
     3961 tgttttttgc cttgttttgt tgttgcaggg tgacatgata atttctctgg tggtggctgc
     4021 cattttttta attcgttatt tcacacaact ttatattgtc aatcgtacag caaagcattt
     4081 taacgaacgc aggttttttc tgcttttacc gatttttgac cttgttcttc ccatcctttc
     4141 gctcagtatt tttatcactg gaaaagcgtc ccgaagaaaa aacaaaaaat ggtaatttct
     4201 gaaagattgg ctcttttcag gctttgaaaa aagtgaaatg aactcttcca tattttcgcg
     4261 tttccatgaa atggggatgt tgcgaaaagt tgtatttgga tgaatgttcg agaatgaaaa
     4321 ttccttccgt tttcaacaat tgctgtgaaa aaatcatatc cggaatttct ttcagatttt
     4381 ccagctggta gggaggatcg gcaaaaatca agtcaaatcg tgaggctgta tgtgtaatgt
     4441 atttgaaaac gtcggttttc aacaaaatca ggttgtcaat ttcaaatttt tctgaaattt
     4501 tttttataaa aaggcattgt tttggattgt tttcaatgct gacgacctga cgacaacctc
     4561 ttgacacaaa ttcaaaacca atgctgcctg ttccggcaaa caaatcgagt acttcaatgt
     4621 cttcaaaatc aattctgttg ttcaatacat caaacaaacc ttcttttgca aagtcggtag
     4681 ttggacgtgc cgaaatgtca accggcgcat aaatatttct tcctttgagt ctaccgctga
     4741 taattctcat tatatattac ttaatcaatt gtttcacatt gttttacgaa ttttctaaca
     4801 aatccttgca gttttgaatg ctgttccatg ttgtaaagat aaaccttaca attttggaag
     4861 tccagattca attcttctat gattctcaaa agataataca caaaatcttc ctgcgtttga
     4921 tagtcgaaac tgtttaccaa caaaagtcgg ttgtgttgaa aagctaccac gtccatgatt
     4981 ttctgccgta cccatataca aatgcattct tgcagcgaat gtttgcggat gtgattttca
     5041 ataaaatgag aaatggaatg ctctattttg caagcaggga aaagctgttt gaaaacttcc
     5101 gaaatgtcct caggcaaaga aaaaatgttg atggcatcaa aagcggagat ttcattgtat
     5161 aatatggaag catttttttc cttttgatga gaaaagtaaa gaagtttgtc catttcttct
     5221 tccttaaaaa tttctttggg tacaatggtg tacgttttgg tttcggcaat caaatgaatt
     5281 ttgtcgtaaa gcagttgggt ttcgggttct tgcagcagca aaggaataat ttgttccttt
     5341 gtcatggaga aaagcggcat attcaatctt tttgcagaaa caagttttgt gttgtctctc
     5401 tctagaatag acaaagaaag tccacccggt gaaaaccgga tggacaaact ataaggttgt
     5461 atataggtat ttgtagattc taagtcgccg cgggatgaat acattttttc atttatctct
     5521 gaaagactta caggatttat tcccagtttc ctgcattatt attgggttct tcaatagaac
     5581 cgactttcag acctggaaat ttacccatct ttaaggttcg atctatcaaa ttaagtaatt
     5641 cctgacgatt cagatcgtaa agataacttt catagagagc actggcttca aacaatggaa
     5701 tgcgaattcc tgatatattt gtgtagttgt tgttaacttt taaagtaaac tcctcatttt
     5761 ttgaatatgg aataatacgc attttgtcaa tgttgtctct attgtagttt gggaaaactt
     5821 cttccaacaa atcggtcaga atggtatcac gcctaaaatt ttgaagacca tattctgcaa
     5881 tttctttttg atttcccttg cgaacaattt cagctgcttt tgcttcggtt aaccctgctt
     5941 ccaattgctt atccgtaaga aaaccttctt tcatcacaat tttctttttg gctgttttca
     6001 aaaaatgaaa caaggtgtca aagctcgctg tgtaacgttt gttttgatct ttaaattcta
     6061 tttccgcttt acgaatatct accaaacact gaataacatc tttttctctc tgattcttaa
     6121 tcctttcgaa acgaatagga gtaatgatac tagctacaca aagatacgaa agtaaaacaa
     6181 tagaaagaga aagcaaaatt ttaatgactg ttttcataat tgaaagattc aattgttttt
     6241 taaaatactt attttatgga gaataacgct acgctattaa ctttttgcaa atttataaaa
     6301 gaaaattcaa tactccatct ttctttcaat attttttacc ttatttttaa tatcgcattc
     6361 aagtgcttac tgttagaacg gattttgagt aacaattgat ccccttccat acagaattaa
     6421 cttgctcatt tttcataaac gcaaaaataa caatttgttt ttaaaccttt ggcttttttt
     6481 cggattaaca aaacatgtaa tcgttatatc ggagttacgt taaattaata atgcatttaa
     6541 tttcaaattc aacacactaa tataaaaatt tcgatgaaaa tggaaatttt tataaacatt
     6601 aataaacaca catccaaata ttaaaaaata ttatttatat ttggaatcaa attgttggaa
     6661 atttcttcca ttttgaaacc ttaagtcttt ttgcaacatg aagaaaatgc tttgtctgtt
     6721 gttgattgca agcacagtat cccaaatttt tcctcaaatt tcttcaatta ttccggtttc
     6781 ttcttctatt gccggaacct ccgtagccaa taccggcctg agatccgaat ttcaaaatcc
     6841 ggccatgttg ggttatgcca agaacagtga aattggtatg caacatgaaa accgatacgc
     6901 actttccgag ttgtcaacca acagctttca agctcttttg agtaccaatg ccattaacgc
     6961 cgatttgtcg ttttcttatt ttggttattc attgtaccac gaaatgatga ccggtttggg
     7021 tttcggaaga aacttttccg acaaatttgc attgggtgtg cggttcaatt attacatggc
     7081 atatttttct gcatccaatt cgtatcgtgg agcattgttt ccccaaattg gtttgtcata
     7141 caggttttcg tcgttattca gtttgggctt cagtactttt aatccctttc agacaaacat
     7201 aaaaaccgat tttacacaaa aaagaattcc ttccattttc agtttgggga cggaatatta
     7261 tttttctccc gaactggtgt ggctggctca aatcgacaga gaaatcagca gcaattatcg
     7321 tttggcaaca ggtttcgaat accaaatgct tcagcaagtt tctctaaagt tgggcgttta
     7381 cgatgccggt tatctggttc cctgcggagg atgtggactg aagtggaacc gttttttgct
     7441 ggatattaat tgcgaattgc atcctttatt gggattgaac tcctatgccg gacttcgata
     7501 tatattttct cgttaatttt ttagtttaac gtccctatca tgaagcgaat tgtcgtatta
     7561 tgctgtttgc ttttccttct cataaatgca gcctatgcac aagatgcgtc tactactatc
     7621 gatcagacaa tagccgatat ttttgaacag tggtctgcag aatcggaaga agttcccgat
     7681 tatcaaacat tttacgagga attggaattt cttgccgaaa atcccattaa tctgaatcat
     7741 acgtcgagag aagaattaag taaaatgctt tttctgtccg atgtgcaagt cgagaacatt
     7801 cttgcttatc tttatcagta tgggccaatg gaaaatattt acgaactaca gctgatagat
     7861 gggctggata tgacggacat cagacgaatg ttgccttttg taatggtgaa aaaagaaaaa
     7921 gcggtttctg aaaaaatcta tcgttatgac gttttgaatt acgggaaaaa cgagttgtac
     7981 atgatgttca acagagatgc ggaaaccaga caaggctatc aggcatccgt ttcatcaatt
     8041 ggagattcaa caaatcaggg gaaaaaatat gtaggttcgc cctggtacca ttctgccaaa
     8101 tatcgttttc attttaagga tagaattttg gccggaatga ctttcgagaa agatgccggt
     8161 gaatcttttt ttggttctgc ccacaaaggt tacgattcct attcgggtta tcttcaaata
     8221 aatcgtttgg gaattttcaa aaccattgtt ttgggtgatt tcagagccaa ttttggtcaa
     8281 ggtttggtga tgaagtccga tttcagtatg ggaaaatcct cctatgtcct taatgttgta
     8341 tcgcgcaatg aaggactgaa aaaattcagt tctaccgacg aatacaattt tttcagagga
     8401 ggtggagcca caattcggct acccagaaat tgggaaactt ccgtttttta ttccaacaaa
     8461 atgatagatg ccgatactgt cgggggcgtc ttttcgagca tttataaaac aggttatcat
     8521 cgtaccgaaa acgaattgaa taaaaaacat accatttgcc aacaggtagc aggcggaaat
     8581 atttcatggc ggttgctttg gctgcaaacc ggaataactt ttgttcacac caattttgat
     8641 caggcgcttg ttcctgaaaa aaccacctac aatcgttttt atttttctgg gaaagcccaa
     8701 aacacagccg gatggaatta tcgtgtccat tggcagaaac tcaatctttt tggtgaagca
     8761 gcagttacag gcaactttca tcctgccttg ttgagtggat tcacgctgtc tcctctttca
     8821 ctagtcaatt tggtagctgt ttaccgatat tattctcctg aatacgatac tttttatgct
     8881 ttggcgcttt ccgaaacttc acgcacaaac aatgaaagtg gtttgtatct tggagccgaa
     8941 ataaatccgg caaaaaaatg gaaaatttct gcttatgccg acagttatcg gttcccttgg
     9001 ccaaaatatg gaatcgatgc tccatcggcg ggaaaagatt atctttttca gaccgatttt
     9061 tctgcccatc ggcgtttatc catgttttgg cgattcagac acagcaacaa accggccaat
     9121 atttcacagg agaattcagt tatgcctgtt gtaaaagcgg tcaagaaaaa ttcattgcgt
     9181 tatcagttga tgtattcgca gggaaatttc agtttcagaa ctgcggcaga aggaaacatg
     9241 gtacagaagg gagaaaatgc cgattatact tacggattga ctgcctatca ggatttgact
     9301 tatgagtttt cttccttacc gttgaagctt gatttcagat accagttttt cgatgcggtt
     9361 gcctacgaaa accgcttgta tgcctatgaa aaagatgttt tgtatgcttt ttccatcccc
     9421 atgtattatg gaataggcag tcgttattat cttaatctga aatatgagct gaaccggcag
     9481 tttactttat ggttcaaaat agctcaaacc gtatatgccg acgatcgtga agaaatcagt
     9541 agcggaaacg agaaaatttt aggcaacaaa ataacggatg tgaaatttct gctgcgtttc
     9601 aatttttaaa aaacaggatt ttttctgcta aatatcgttt cagggttgat acgggctgat
     9661 acattttata actatcattt tttatgcttg ctttacaata atagtaatgc atgaacttta
     9721 taaacttttg tggctattaa ctatatattc aaattttatt gcaataatca ataaaaaata
     9781 ctgtactctt ttaaaaatat catttcttta ctattttcaa gctcaaaatt gccaatttcc
     9841 tattaaattg taggcaatat catttctatt tcccttattc cattttctac ttcttgtata
     9901 tttactttca ctgtagagtc tggtaaaaga gcttcaattt tttcaggcca ttcgatgaaa
     9961 caaatttttc cgctataaaa ataatcttca gctcctaagt cgatggcttc ctgtaactta
    10021 ttaatacggt agcaatcaaa atggtacaaa actcgtccat ctgctgcttc atattcatta
    10081 ataatggaaa aagtaggact attcactgta tctttcactc caagaatttc acaaacagct
    10141 ttaataaagg tcgtcttacc ggctcccata cttccataaa aagcaaaaac cgagtatcta
    10201 tcaaataaat taataaattt tcgcgctggc tgctgaatat tattcaagtc aaatatttct
    10261 gtcatgttca atttttaatc tttaaagtta ttacaaataa aaatttattg accaaaatac
    10321 actcattttg aaatttgcga ttctggagca ctcaaatctt tgtgaaaata cccataaact
    10381 attgatgctc tgtgatttcc gacaactttt tgaatatctt ccagatttgc attctggagt
    10441 ctttttatac ttttaaaatg atttatcagt tctcttttgg tcttatcccc tattccttta
    10501 atattatcta gttcagatgt ggtttgattt ttacttcttt tctcgcgatg aaatttaatg
    10561 gcaaagcgat gcacttcttc ttgcattgta gccaacaatt tgaaaagagg ttcattgggt
    10621 gtcatgccaa tattttttgg aggaaagccg aaaagaattt cgcgagtatg gtgtttactg
    10681 tctttggcaa gtccaataat gggaattctt aaattaagct catcttcgac aacttccctg
    10741 atcacttcca tttgtccaat tccaccatca gctatgatga gatcgggcaa ttctccgttt
    10801 tctgctatca tccgagagta acgtcttttt actacttctt tcatggaagc atagtcatcg
    10861 ggacctacaa ctgtcttaat gttgtatttt cgataatctt tttttgaagg tctccccttt
    10921 ttgaaaacca cgcaggctgc tactgcatct ttcccctgaa tattggaatt atcaaaacat
    10981 tcaatagtta ttggtaattt ctcaagatgc aatttctcct gaatttcttt taatagctgc
    11041 atacttcttt gatcaggatt taatttttca gcttgcttaa gtttctcaaa cttatattgc
    11101 agcacatttt gctgagccaa atccaagagt tttttccggt ctcctctttg cggaataaca
    11161 atgctaacgc cattcggctt ataattgatt tcaaaaggta cgacaatttc tttggagtta
    11221 ctttttaatc tctctcttaa ttctactatg gccatagcaa gtaaatcttc cttttcctcg
    11281 tcaacatgtt tttggtactc gatggtatat ccctgaatta ttgaaccttt tgaaattctc
    11341 aagatattaa tataagcagc attttctgtt tcttcataac caaacacatc tgtgttttct
    11401 accactgaat tggcaatgat cgactttgat ttgtaatttt caatcaaatc atatttttgt
    11461 ttgatttgat atgcttcctc aaatttgtaa gcagcagaaa gcttttgcat ttcttcccaa
    11521 agcattttgc ttatttcgtt cgcattaccc tcaataattt gcttaatttc gtttatgtat
    11581 ttccgataaa tttcagcaga ttcttttcct tcacacggcc cattacactt atggatatga
    11641 tattgaaggc aaactttaaa ttttccagct ttgatgtttt catcggtcaa ctgaagtttg
    11701 caagttcgga tgggataaag atcatgtatt atctctaaaa gagaattgat cgtgtaactc
    11761 gaactatagg gtccaaaata taaggaacca tttttaataa tatttcttgt tttgaatact
    11821 ctgggaaatt cttcattcgt tatacaaatg cttggatagg tttttccatc cttcaataaa
    11881 acattgtaac gcggttgaaa tttttttatt aaattgtttt ccaacaacaa ggcatcttct
    11941 tctgtgtcaa ccacgatata tttaagagat gagatatttc ttaccaatac tgccgtttta
    12001 cgtgattcgt gcgttttggt aaaataggag gaaacacgct tctttaaatt tttagccttc
    12061 ccaacataaa taatctcctc cttttcattg aagtattggt aaacacccgg cttttcgggg
    12121 agagcatcaa tttgctgctt taattgagga tgcaaattca taatactttt gaatgtttca
    12181 cgtgaaacaa agttagaaaa aattctttaa tttcccgatt gttccacgtg gaactataaa
    12241 attatcgtcc cattagaact aaaagtatat taatgtcact tggagaaatt cctggtatcc
    12301 tactagcttg accaatagtt tcaggatcaa tttttgacaa tttttgtcgt gcctccgttg
    12361 ataaggatga aatagtatga taatcaaatt ttcctttaat attgatgtgc tctaacctgt
    12421 gaaacttttc tgcaatagat ttttcgcgat ctatatatcc tttatatttc aaagaaattt
    12481 ctgctgcttc gactatttct tcaactctat ctcctattga ttgaattttt gattttaaag
    12541 caggtacaat ttcagataat tccataatag aaatctgagg acgaagaatc aaatcaacaa
    12601 gtttacaacc atgagttaac tctgaagaat tttttgacac taaaaaacca ttcacttgtg
    12661 caggtttgat tgagaaattt tcaacaaaag tttttaaaga ttctatagcc tcgtatttga
    12721 aagaatattg atttattcga ttctttgtaa ctaatccaat ttcataaccc ttgggtgtta
    12781 accgtaagtc tgcatcatct tgacgtaaaa ggatacgata ttcagcacgt gaagtaaaca
    12841 tacgataagg ttcgtcaact cccttagtaa ccaaatcgtc aattaaaact ccaatatagg
    12901 cttcatcacg atccaataaa aattccttcc ctccatgaca atttatatgc gcattgatac
    12961 ccgcaattaa tccttgtccg gcagcttctt cgtaaccggt agttccattg acctgtcccg
    13021 caaaaaataa atttttaatg cgtttggttt cgagtgtatg ctttaattgc gtaggatcaa
    13081 aaaaatcgta ttcaattgca tagccgggac gataaagttc ggctttttca agacccggaa
    13141 tcgtatgtat agcttgaatt tgaacttgaa caggaagaga agaagaaaaa ccgtttaaat
    13201 agtactcttg tgaatcaact ccctctggtt ctaaaaacag ctgatgagaa gatttatcgg
    13261 caaaagtaac aattttagtt tcaatacttg gacaatatct cggaccaata ctttttattt
    13321 gaccgttata aagtggagat tcgggcaacc cttgtcgtaa aatgtcatga gttaattcat
    13381 tcgtataagt tatccaacaa ctcatttgtt tcaattctcg tgttacttca gtgagaaaag
    13441 aaaatttatg aaaatcgttt tctcccggtt gttcctttag cacagaaaaa tcgattgttc
    13501 ttccatcgat acgtgctgga gttccagttt tcattcggcc ggtagtgaaa cccaatgatt
    13561 ttaattgctc tgtaatacca aaagaagcag gctcagaaat tctacccccg attagttgat
    13621 ttttaccgat atgaataagg ccgctcaaaa atgtaccggc tgttaataca acacatttag
    13681 ctgagatttc tattcccaaa gcagttaaaa cacctgtaac ttgcccattt tcgataagaa
    13741 tttgctttac tgtatcttgc caaatataaa gattaggggt attggttagc gtcttaatcc
    13801 actgaagaat aaattgatgt ctatcacttt gggagcgagg actccacatt gccggacctt
    13861 tagaacgatt aagcatacga aattgaatgg cagagcgatc ggtaacaact cccatttgac
    13921 cgccaagcgc gtctatctct ctaacaatct gaccttttgc tatgccgcct actgcaggat
    13981 tacaactcat ctgaccaatt ttattcatat ccatagttat gagcagcgtt ttagatccca
    14041 tattggcagc ggcacaagca gcttcgtttc ctgcatgacc gccaccaatt acaattatat
    14101 catatttaaa atccatatcg agtaattaaa ataatatccg agcagaaaat aacaaaagaa
    14161 taaagtatta gttcaaaaaa tagaataaaa aaatatcaag caaataaagc tttgccaatt
    14221 atggtggaaa tttgctccag ataataaata tcagcttcat caagaagacc aaatttttcg
    14281 ctatctatat caagaacgcc aaccacttct gaagaagaat tgtacaaagg aacaactatt
    14341 tcggaaaccg aaatactgct acacgctata tgtccagaaa aatcattaac gtcaggaaca
    14401 agaagactgc gtttttcctt ccatgcagtt ccgcaaacac ctttaccaaa ggcaattcga
    14461 gtacaggcaa caggaccttg aaacggccca agcacaagaa aatcttcctt aaccaaataa
    14521 aagccaaccc accaccattt aaaagtctga tgaaaaacgg ccgataaatt agcaagattt
    14581 gctacaatat ccggttcatc agctataaga gcgggaatct gcagtaataa actctcgtaa
    14641 atggattttt tatccagaga agaactaata atgaaatttt cagacatcac aaaaaatttt
    14701 ttttgcaaaa ttacgctaaa aaaatcattt ttatgagtta ggaaagaatc taacaaaaaa
    14761 agaaaaccat cgtttaacat tcttatttaa aaatttcaaa ataattttct tttctaaaaa
    14821 attatcaatt actttgctaa caaataaaaa cagagacagt cataaattaa aaaaattatt
    14881 tttgaaatat aaacaacaaa aattatgaga aaattagagt taaaattttt tccttttgta
    14941 ttattgattt gtctgaatac atcaattttt gcacaacaag tgcagttcca acctttgccg
    15001 atagacccaa aagtcaaata tggaaaattg gaaaacggat taacttatta cattcgttca
    15061 aatcaacaac caaaaaacag agcagatttt tatatcgttc aaaatgtggg tgcaatactc
    15121 gaaaacgatg atcaaaatgg attagcgcac tttttggagc acatggcatt taatggcaca
    15181 aaaaatttcc ccgggaaaaa aattattgaa tacttcgaaa gtgtaggagt aaaatttgga
    15241 gctaacatca atgcctacac gtctctggat gaaacggttt atcatttatc gaatgttcca
    15301 accaacaggg aaagtataat agacagtgct ttacttgtgt tgcacgattg gtcaaatgca
    15361 atttcgctgg aagaaaatga aatagaagca gaaagaggag ttattctcga ggagtggaga
    15421 cagggagcaa gtgcggatcg aagaatgtgg aaagaatcaa acaaacaaaa attccccggc
    15481 tcacaatatg ccaaacgtga tgtaattgga gatactgcgg ttataaaaca ttttacgccg
    15541 gaagcattac gggattatta taaaaaatgg tatcgacctg atcagcaggc aatcattgtg
    15601 gtaggagacg tagatgtaaa caaaattgaa gaaaaaatta tcgaattgtt ttccaatatc
    15661 aaaaaaccgg aaaacacacc tgaaagacca atttatccaa tatacgacaa caaggaacca
    15721 ataatctcta tagttactga cccggaagcc agaatgacaa gagtggaatt aatttataaa
    15781 cacgacaagt taccggcaga aattaaactg tccataaacg gctatctggt gaatgtgata
    15841 aattcgttaa tttcaaatat gatgcaaaac agatttgaag aaataaccat gcagcccgat
    15901 gcaccttttg tagccggata tgcaacgtat ggagaactgg tcaaatcaaa agatgcattc
    15961 tatctggtag tagtaccaaa agaaggctca gaaactgaag gaattaaaag cgttttaatt
    16021 gaagcagaaa aagttaaacg ttttggattc aatcattcgg aattggaaag agcaaaagcc
    16081 gatttggcaa aaatgataga aaaacaatat aatgaaaaag aaaatcagaa aaatgaaagt
    16141 tatgtgagag aatatatgaa tcattttctg aatcaaaatc ccataccggg catagaatgg
    16201 gaatacgaaa caattaaaat gattttgcca caaataaatg ccgatatggt gaatcaactt
    16261 gcaaaatctt acgtaaccga cacaaatatg attgtttcga taatggcgcc tcaaaaacca
    16321 acagtagttc ttccaagtaa agaagaaatc aggcaaataa ttgaagaagt caaaaaaacg
    16381 gagataaccg ccaaattgga cgaaaaaata gataaacctt taattgaaaa aatgccaaaa
    16441 cccggtaaaa taaaaaaagt atcaaaaaat gatattctgg gaacaacaga atggacattg
    16501 agtaatggtg caaaagttat actaaaacca accacattca aaaaagatga aatattattc
    16561 aatgcctaca gcacaggagg cctttctaaa gttgaaaata ttgagaattt accttcggct
    16621 gaatttgcaa cagaagttgt aaacaacaac ggcttgtcca atttcaatca aatagagttg
    16681 aaaaaattac tttcgggaaa aattgcttcg gtcagtccat atatcgataa ttatgaagaa
    16741 ggcttctcag gaaattcttc cgtagcggat tttgaaacaa tgttacaact tctatattta
    16801 cattttactg ctttaaggaa agatgataac gcctttacgg ctctgatgaa caattataaa
    16861 gccttcctgg caaacagcga gaataatccc gacaaggctt tttccgattc tgttcaatta
    16921 atgctttcca accacagtcc ccgtacggtg cttttaaata tgaaaacatt ggaaaaagtg
    16981 aaccaggata aagcattgga aatatttgct gagcgatttt cagacccggc cgactttaca
    17041 tttgtgttca cagggaatgt tgatccaaat gataaaacgg tagtcaatgc aattaatact
    17101 ttcattggcg gaattaaatc gaaaggcaaa aaagaatcgt ttactgatga taatgtaaga
    17161 cttccagttg gaagagccat caaccatttt acaagagaaa tgcaagtcaa aaaggtttca
    17221 aactgcattg tttacagcgg aaatttgaat tttaacatga ataatcgaac aaacatgaat
    17281 gctataggaa atattttaaa tattcgctat cttgaaagtg ttcgagaaaa agaaggtgga
    17341 tcatatggcg taggcgttgc tggcaatttg agaaatcgac cgattgaaca ggcaatgctg
    17401 ttcattcaat tcgatacgaa cattgaaaaa caaaacaaat tgatggatat catacattcc
    17461 gaaatcgaca ccataatcag caaagggcca aaagcagaag acttacaaaa ggttaaagaa
    17521 acaatgatga agaaatacga ggaagatctg caagaaaata aatggtggca aagtgcaata
    17581 aaaaattatt atttcaataa attgaatgta gttaacgact atcttccatc ggttcaagca
    17641 ctgacaagtg aaagtattca aaaaacactg aaaactatcg tggaacaggg caatgtaatg
    17701 gaaatagtca tgaaaccaaa agaataaaat caattgcagc tcaataaaaa aatttctctg
    17761 caagaattca gtaaaaaaaa atcttacaga gaaatttttt atttatacaa ctgctagaat
    17821 taccaaatgc aggcgcgctt ttctacaggc aaatacaatt tatctccttc cttaattcca
    17881 aaagcatcat accatgcctg aatatgaggc aaagccccat ttaccctcca acgacccaaa
    17941 gcatgcggat cagactttgt caaaacaaga atttcttcgg gacgtatgtt gcaagcccaa
    18001 acattagcat aagacaaaaa gaaacgttgt tcgggagtga aaccatccaa aattttcgat
    18061 ggctggcctt tggttgaatt tttataagcc tgaaatgaaa cttgtaaacc tccatgatca
    18121 gcaatgttct ctcctaaagt aaatttcccg ttagcatgga caccgggagc aacttcaatg
    18181 gaatcgaaat aatcggccaa aacttttgcg cgggcatcaa actgcttctt atcttcttca
    18241 gtccaccagt tttttaaatt tccatcctta tcgaactgac aaccctgatc atcaaaacca
    18301 tgcgtcattt catgacctat cacaacgcca atggctccat aattaacagc atcatcggca
    18361 tggacatcaa aaaatggagg ttgtaaaata ccggccggga aacaaatttc gtttgtagaa
    18421 gggttgtaat aagcattaac agtttgagga gtcat
//
LOCUS       DAOSIB010000041        17523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_3454_length_17523_cov_11.504637, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000041 DAOSIB010000000
VERSION     DAOSIB010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17523
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3454_length_17523_cov_11.504637"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..850
                     /locus_tag="PLD44_05165"
     CDS             104..850
                     /locus_tag="PLD44_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98756.1"
                     /translation="MRALRKIMTGIILTVIITSCGNSSSGSKSGNEPDVLKEEIPQLQ
                     TRTIDVGKLKMHQDGYSIGEKTELTELQKQIKDEEVGTIEITWIWDCSPKYADMPMKI
                     VYNKIDGVLKLIYTQNNVIEEYKNIMPECLSEFLKNGEKNFYSLESYCKDAKYDFNNR
                     EMTNKAVGNKPAQSELDGSVGIVKDYIKENANDASSIKFIEWSKVSSFGEYWVVRCKY
                     KGTNALGGVVTENMWFYIQNNQVIKSKPIE"
     gene            1012..1629
                     /locus_tag="PLD44_05170"
     CDS             1012..1629
                     /locus_tag="PLD44_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98757.1"
                     /translation="MKHLKILTIIVLTLIWTCGFGQTDIDNEIISALINSEFKSLPND
                     TIFNRKGEIKRIKTYKKTDILLIIETETFLFDPEVDSFDRFKNETNGLPSLDSLTYLD
                     FIDKNKTKIQIDSIYGFQGTITYNSRQEIDNIFKQGGWDNYHKIHGYKSMIKVSRPGL
                     NKNMTKAFIYYSSSSGGLSGAGFYLILENIDGKWIVKESMLAWIS"
     gene            complement(2352..3797)
                     /gene="amrB"
                     /locus_tag="PLD44_05175"
     CDS             complement(2352..3797)
                     /gene="amrB"
                     /locus_tag="PLD44_05175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOF98758.1"
                     /translation="MEKFNETIDRRPAVAGKFYPANVEELQKEIDIFFKNAAPKQLDR
                     VRAIICPHAGYVFSGKITASAFNQIDANREYKRIFLIGSSHHKYFDKASVYCSGNFLM
                     PYGEEKVDMDFGRKLVQNFPEWFTDDRTPHLNEHCLEVQLPFLHHVLRKNYVIVPILI
                     GTAHPSVCKQIASVLKPYFNEENLFVVSSDFSHYPAYQDAKYVDSLTKDAICSNRPEK
                     LLETLAENERKHISNLQTSLCGWTSVLTLLYMTSSNKSLKFHAVDYCNSGDVKYYGER
                     DRVVGYWAIAVAESKHAAEDFYLTEKDKKDLLKISRKTLEEHCRAGRVSEVDTATLSE
                     TVKKNCGAFVTLHKRGALRGCIGILTAEKPLYKTVQEMTIAAASHDPRFMPVEASELP
                     FIDIEISALSPLKKINDISEIELGKHGILIEKNFHRGVFLPQVATETGWSKEEFLGHC
                     SADKAGLGWDGWKTADIYIFTATVFSEKDFW"
     gene            4390..5241
                     /locus_tag="PLD44_05180"
     CDS             4390..5241
                     /locus_tag="PLD44_05180"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HOF98759.1"
                     /translation="MDKKYNALSFSTSKFVTKFYSTSFYSASQYFSKEIQEAIFSIYG
                     FVRLADEIVDSFHFSDKEYLLRKFESDYYDAVKNGISLNPILHSFQMTVGKYGISDEY
                     VQHFLSSMKADLEKKEYKSQIELNTYVYGSADVVGLMCLKVFCNGDEQLFDRLMLPAT
                     KLGSAFQKVNFLRDIKQDTQELGRHYFPQIVEEGLTEQTKKELVEDIRKDFDDAYKGI
                     VLLPADSRLGVLIAYKYYTALLRKIEKTAARDILNKRIRISDAGKMFLVLSTQIKYYL
                     RAFYGNK"
     gene            5228..5782
                     /gene="idi"
                     /locus_tag="PLD44_05185"
     CDS             5228..5782
                     /gene="idi"
                     /locus_tag="PLD44_05185"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOF98760.1"
                     /translation="METNKENFVQLVDENDQPVGIMEKYEAHKKAALHRAISVFIVNS
                     KGDWLIHRRALDKYHSNGLWTNATCSHPYPDESYLEAAKRRLKYEMGINCELIPLFHF
                     IYQEKVNDELTEHELDHVFFGVSDDVPIHNTGEVEEWKYIPYRQLLEDVENHPEKYTV
                     WFKIILKKVKAYIDTENLTESSCR"
     gene            5773..6438
                     /locus_tag="PLD44_05190"
     CDS             5773..6438
                     /locus_tag="PLD44_05190"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HOF98761.1"
                     /translation="MQINLLLEKFRQKYPERKVRQFIFTFYAVGIVGLALPATHVFFV
                     YLIPLALLLSAFILLPFQEDDFSIKTGVLFTVIFVVSFTVEAIGVNTGKIFGQYFYEN
                     GLGVKCFGTPLIIGLNWALLVYLTASIFQSFRINKFFLVLFPSVLMVLYDVVLEQVAP
                     KMQMWFWNADRVPFRNYLVWFLMALIFHTLLVISGLKLKNPLSKIVFSAQFLFFLILM
                     FIL"
     gene            6448..7074
                     /locus_tag="PLD44_05195"
     CDS             6448..7074
                     /locus_tag="PLD44_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOF98762.1"
                     /translation="MLKAKHHCLIYPFFRRYTLLLMKRNFHSVNVIRTNFSRNENLPV
                     LMIANHISWWDGFWAMYLNLKVFHKKFYFMMLEDQLLKFRFFQWTGGFSIKKKSREMI
                     ESLQYAAKILDNPDNLLLIFPEGKLQSLYNPLHVFEKGIERIVEGHKNNFQLVFVANF
                     IDYLSHKKPDLYMYVSEYQGNVFSHSKLQEAYNVFYGNCLDIQKSKTE"
     gene            7078..8169
                     /locus_tag="PLD44_05200"
     CDS             7078..8169
                     /locus_tag="PLD44_05200"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOF98763.1"
                     /translation="MIYLAWVVFGFTFFQLCIAGLNYFFREKIPISTNRISDEKISVL
                     IPARNEEQHIGNLLNDLSGEDDNILEIIVFDDLSSDHTVEIVNQFIGTNEKIRLIRST
                     HLPAGWLGKNFACYSLANAAKGNYFLFLDADVRMEKEIVVSSVKFFKQKSLHLLSIFP
                     TQLMMTKGEKMTVPNMNYILLTLLLLPLVRKLSFPSISAANGQFMLFDADTYKKFQPH
                     EKFKDKRVEDIEIARFYKKLKLKIACLTGIKQVRCRMYENFAEAVNGFSKNVIHFFGN
                     SFLIAVLFWIVTTFGWLAVWLAFGLPTLAVYLLAVVCIRIFFSLASNQSILDNLIFFI
                     PQQFSLGIFIYQALINRSKNQYLWKGRKV"
     gene            8148..8897
                     /locus_tag="PLD44_05205"
     CDS             8148..8897
                     /locus_tag="PLD44_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98764.1"
                     /translation="MERKKSLVLFFLLILLHLHVHASHRENIYKAYINNDMQAWKNTI
                     DKMNLSSGNASDEFLLELVNYEYGYIGWCIGNKKTAEAKEYLKKAENRLITLEKNQFR
                     LSMIEAYKSAFYGFQIGFNKLKAPVLGPKSVEAAKLAIQYDPNNYFGYLQMGNIYYYS
                     PAAFGGSKAKAIESYRQAEKLILSNNHRLKNDWNYLSLLVTLAQAYENTGNDESAKIY
                     YQKILNVEPDFAWVKNKLYPEFLKKTQKNNI"
     gene            8894..10363
                     /gene="crtI"
                     /locus_tag="PLD44_05210"
     CDS             8894..10363
                     /gene="crtI"
                     /locus_tag="PLD44_05210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOF98765.1"
                     /translation="MKEKVIIVGAGLGGLATGLRLAKEGYEVEFLEKNTKAGGRLNQI
                     KKDGFTFDTGPSFFSMSYIFKEFAEKCGINLPFEFIELDPLYSVNFSGEEKTYFLYKD
                     IHKLAKQFDDVEPDFELKMEKYLQKSAQLFNDTVDIVIKRNFDSYLDYFLALMKVNPV
                     HLPILFKTFWQHVCDYFTSKQAREIISLVAFFLGRTPFDTMGIYSLLSHVEFRHDGYY
                     NVKGGMYKIVEGMLEELSKLDVKFHYQTEIVAYHAENRKIVSLKDAQGNLWKADKFVI
                     NADAALFRSKVMKYKNFSEKKLDKMEWTMGYLTFYLGLKCKLPQVNHHNYFLGSNYEQ
                     YAGNILQNPDTLEKPYYYVNVVSKYNPECAPEGCESLFFVCPVPNLKYKNDWSDREKI
                     VNSIIDDFSQRIKKDISAEIISKTIFTPQEWRDKFSLYQGSGLGLSHKFTQIGAFRPK
                     NYDEKHDNLFYVGASTIPGAGLPMAVISAELVVERILGK"
     gene            complement(10487..11431)
                     /locus_tag="PLD44_05215"
     CDS             complement(10487..11431)
                     /locus_tag="PLD44_05215"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOF98766.1"
                     /translation="MAASNSSFKVFSGTNSRSLAEKICQSLGCPLGKMNIEHFADGEF
                     AVSYEESIRGLNVYLVQSTFPNSDNLMELLLMIDAAKRASAYKVIAVIPYFGWARQDR
                     KDKPRVSLGAKLIADMLSVAGIDRLITMDLHADQIQGFFNVPVDHLYASTIFAPYIQT
                     LRLKNLVIAAPDVGASKRAGSYSKYFDVPMVLCHKTREKANVVGEMRIIGDVKGKDVV
                     IVDDMVDTAGTLCMAANLMMEQGANTVRAIVSHGVMSGSASEKVEKSALTELAFTDSI
                     PFDASKCSKVKILSIADMFADTICRVQEHKSISEQYIF"
     gene            11725..14421
                     /locus_tag="PLD44_05220"
     CDS             11725..14421
                     /locus_tag="PLD44_05220"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOF98767.1"
                     /translation="MKIHFKIPYYTHWGQQVFVCGNLPELGNNVPEKSFQLHFQNTED
                     WYGEIEIHQNEPFELEYRYLLYEENTKRFTYEWIEDRKITIDPAKMEHVFCFDFWNAP
                     GAVENVFFTAPFKKVFFSDNFNHSQKTRSKKSFTHIFRIKAPLLKNHQVPCLIGDCKV
                     LGSWNTVSPLLLTRDGENWWKAEVDLGKEKSDVHYKYGIYDTENGQFLMFENGFDRTA
                     PVFNAKNTFVRLSDGFLRMNSQSWKGAGVSIPVFSLRTKESFGVGDFGDIRLLVDWAE
                     KAGLKLIQVLPLNDTIGTHTDADILPYAAISAFALNPLYLNLPSLGKLPENHPLQKEY
                     EERQPQLNALEWIDFLEVVNFKLKYVKALFSFHQEEFLQNKDFLTFFENNKHWLIPYA
                     AFCVLRDRYGTPDYHQWKEFSTFNEEKITGFTSANQPHYADIALNYFIQYHLHIQLME
                     AASYAHQHGIVLKGDIPIGVNKNSVETWAHPELFHIDMQAGAPPDMFSIKGQNWELPT
                     YNWEAIKQTGFDWWKKRLKQMSNYFDAFRIDHILGFFRIWEIPNDQLEGIMGHLYPSI
                     PVYLDEFQAKGIWFDYNRFCKPFINDQVLEQLFGTDAGWVKVNCLQIEDGWILRLKPF
                     YRSQKNVQQLYEEGQFSENIRNGLFDLISNVLFFEVEGSHGTRFYPRWGMHSLSTFQY
                     LDDFTKQKLDEIYIDYFYHRQDEFWFQSGMEKLPVLKQTTDMLICGEDLGMMTPCVTR
                     AMKELGILSLEIQRAPKTNKIEFFHPSDAPYLSVVTPSTHDMSTIRGWWEEDRNITQR
                     FYNTQLGHWGEAPYFCEWWICRDILLQHVYSPAIWAIFQIQDLLGVSEKLRRENPHEE
                     RINVPSDSKKSWRYRLHLNLEDLLKEDDFNRQIRNYIRQAGR"
     gene            complement(14635..17115)
                     /locus_tag="PLD44_05225"
     CDS             complement(14635..17115)
                     /locus_tag="PLD44_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOF98768.1"
                     /translation="MHSRKLFLFVFFSILSISLFSQNKVRIYGYVIDTNNRGIELANV
                     YVQHTNIGTSTNQNGYYELTVDIKDSIKLVFSMIGYQTVTYTVHPKLKVIQLTVELPP
                     LSKQIEDVNVIGQRRQTSTIDVVDPSKYRLMPNSAGGIESLLISFTGVSSSNELSSQY
                     NVRGGNFDENIVYVNGIEVYRPLLIRAGQQEGLSFINPDMVQNVSFSSGGFNAEYGDK
                     MSSVLDISYKKPSAFEATASASLLGASAYMGTAGKRFSQMHGIRYKTSKYLLGTMDTQ
                     GEYDPSFVDYQTFLTYQIKPKWELTFLGNFSQNDYRFIPQTRETSFGTYSMGRKLTIF
                     FEGQEKDLFQTSFGALTLNFRPSKSTKLSLIGSAFYTNERENYDISGEYILSEVKMDV
                     DPDKQTGESLGIGNYHEHARNKLNATVANLSHLGEYAKNGHLLKWGANVQIEKIDDQI
                     NEWEWRDSAGYSLPYNNRQVNLYYNMNASNSLKTLRATSFLQDTYKYSSTAGTFSFTG
                     GLRSHYWTFNREFLLSPRFTVAYLPHWEKDFSFRFATGLYYQAPFYKEIRDTLTDALG
                     NVDIKLNSNIKAQRSLHFVLGGDHYFRAWGRPFKFTTEAYLKLADRVVSYSVDNVRIR
                     YSGENDAKAYTAGIDFKLFGELVPGTDSWINLSLMDSKEDIIGDSYVRHTYDDEGNIL
                     SSETIYPSWVPRPTEQRYAFSMMFQDYLPSNPKYKLQLKLIWSDGLPFGAPRNEQFRS
                     AFRMPPYRRVDIGGSRLLVNGSDEWMNKSWLGHVKNIWINVEVFNLLNFKNVNSYYWV
                     TDIYQHQVAVPNYLTGRQLNLRLLVDFK"
BASE COUNT         5656 a         3118 c         3353 g         5396 t
ORIGIN      
        1 gtattgacag ttttttactc cgaaatccgc cactgcgcca agcgccaaac cgttatgcgt
       61 aatttaaccg aagataagaa aactaacttt aaaacttata aatatgagag cacttagaaa
      121 aattatgaca ggaattattt tgactgtaat tattacatca tgtgggaatt caagtagcgg
      181 aagtaaatct ggtaacgaac ctgatgtatt aaaagaggaa attccacagc ttcaaacaag
      241 gacgattgat gtgggtaaat tgaagatgca ccaagatgga tattctattg gagaaaaaac
      301 tgaacttaca gaattgcaaa aacaaattaa agacgaggaa gttggaacaa ttgagataac
      361 ttggatttgg gattgttctc ctaaatatgc cgatatgcca atgaaaatag tttataataa
      421 aattgatggc gtcttaaaac tgatttatac acagaataat gtgattgaag agtataaaaa
      481 tataatgcca gaatgtttat cagaattttt aaaaaatgga gagaaaaatt tttattcgtt
      541 agagagctac tgtaaagatg caaaatacga ctttaacaat agagaaatga ccaataaagc
      601 tgttggaaac aagcctgcac aaagcgagtt agatggttct gttggaatcg taaaagatta
      661 tattaaagaa aatgcaaatg atgcgtcaag tataaaattt attgagtggt ctaaagttag
      721 ctcttttggt gaatattggg ttgtgagatg taaatataaa gggacaaacg cattaggagg
      781 tgttgtaact gagaatatgt ggttttatat tcaaaataat caagtaatta aatccaaacc
      841 gattgaataa aaataaaaaa cgcataacat acggtataaa aaattgccgg agctgtaggt
      901 tattcaagag ttgtagcccg cctcaacttt tgtgtaaccg gacaggatag tagcccgcaa
      961 tcccttactg gccatattgt caaacgttgt ggccaattta aaaaaacact catgaaacat
     1021 ttgaaaatac tgacaataat agttctcaca ctgatttgga catgcggatt tggacaaact
     1081 gacattgata atgagataat ctcagctttg ataaatagtg aatttaaatc attacccaat
     1141 gacacaattt ttaatagaaa aggagaaatt aaaagaatta agacttacaa aaaaacagat
     1201 attcttttaa tcattgaaac ggagactttt ttatttgacc ccgaagttga ttcatttgac
     1261 aggtttaaaa atgaaacaaa tggactgcct tctcttgaca gcttgactta tcttgatttt
     1321 atagataaaa ataaaacaaa gatacaaatt gattcaattt atggttttca gggaacaatt
     1381 acatataatt caagacaaga aatagataat atattcaaac aaggtgggtg ggataattat
     1441 cataaaatac acggatataa atctatgata aaggtttcac gtccgggatt aaataaaaat
     1501 atgacaaaag ccttcattta ttatagtagt tcaagtggtg gattaagtgg agctggattt
     1561 tatctgattc ttgaaaatat agatggtaaa tggattgtta aagaatctat gcttgcatgg
     1621 atttcttgac caaatgactg attgtgaatg ataaattggc cacaacactc ggtataacca
     1681 ataagggttt cagtggtttg cgaagggttg tagtctgctt caacttttgt gtaaccgaac
     1741 aggaaattat ccccaatcgg caacttttca taccaccgaa cgttgtgtgc aagcttaaaa
     1801 aaagcaataa aaactctatt aaatgtcgaa aaaagaacaa aaatatgaac gaaaattctt
     1861 ttaaggacga tcaaactttg aaaaaataat tttatgttag ctgcttgcca cacgatgcaa
     1921 ttgtgaggaa gaagagttgt atgagatttt tgaactaaac attaaaaaat ttgattattc
     1981 tatctctgac acagttattg aaaagctgaa agcatttttc aacaattcag tagcgcacaa
     2041 ggataaaaac tttggggatg ctcgctttgt cttctttgaa aaaacaactg ttgaaaaagt
     2101 aaaattacac acagcgagtt caaaacctta tccttcaagc aaatcttagt aaccaacaat
     2161 atgtccacgt gaaccaaacc ttaaagggtt gcttcggatt ctacatcctc gcgaggagag
     2221 gactattaaa agtaggaata aaggattgtt gaataatgtt ttgatcgaaa aattatttct
     2281 tgcttttcaa acaaaacaca aataaatact tgattttcag tttaccaaaa ctttgccaaa
     2341 attattctat tttaccaaaa atctttttcg ctgaaaacgg tcgccgtaaa aatgtaaata
     2401 tcggctgttt tccagccatc ccaacccaaa cctgccttgt cggccgaaca gtgtccgaga
     2461 aattcttcct tactccagcc tgtttctgta gctacttgag gcagaaatac gcctctgtga
     2521 aaatttttct cgattaaaat tccgtgtttc cccaattcga tttcggaaat atcgttgatt
     2581 tttttcaggg gcgacaaagc cgaaatttca atatcgataa aaggcagttc ggatgcttcg
     2641 accggcataa atcgcggatc atgggaagca gcggctatcg tcatttcctg aacggttttg
     2701 tacaaaggct tttcggctgt caaaataccg atacaacctc ttaaagctcc tcttttatgc
     2761 aatgttacaa aggcaccgca atttttttta acggtttccg acaaagttgc cgtatccact
     2821 tccgaaactc ttccggctcg acaatgttct tcaagcgttt ttctcgaaat tttcagcagg
     2881 tcttttttgt ctttttcagt gagataaaaa tcttcggcag catgtttact ttcggctact
     2941 gcaatagccc aataacccac aaccctgtcc cgttcgccat aatacttcac atcccccgaa
     3001 ttgcaataat ccaccgcgtg gaacttaagc gatttgttcg aagaagtcat ataaagcagc
     3061 gtcaataccg aagtccaacc gcacaaactg gtctgaagat tagaaatatg ttttctttca
     3121 ttttcagcca aagtttccaa caatttttcg ggacgattgc tacaaatggc atctttggtg
     3181 agggaatcaa catatttggc atcctgatag gccggatagt gagaaaagtc ggaactgaca
     3241 acgaataaat tctcctcgtt gaaataaggt tttaataccg acgctatttg cttgcaaacg
     3301 gaaggatggg ctgtgccgat aaggatagga acgataacat aattttttct taacacatgg
     3361 tgcaaaaatg gaagttgaac ttccagacaa tgttcgttta aatgaggagt acggtcgtcg
     3421 gtgaaccatt ctggaaaatt ttgcaccaat tttcgtccaa aatccatatc gactttttcc
     3481 tcaccgtaag gcatcagaaa attgcccgaa cagtaaacgg aagccttgtc aaaatacttg
     3541 tgatgagagg aaccaataag aaaaattctt ttgtattctc tgtttgcatc gatctggtta
     3601 aatgccgaag cggtaatttt gccggaaaaa acataaccgg catgcggaca aataatggct
     3661 ctgaccctgt cgagctgttt gggtgcagca tttttgaaaa aaatgtcgat ttctttctgc
     3721 aactcttcaa catttgccgg ataaaacttg ccggctactg caggtcttct gtcaatggtt
     3781 tcgttaaatt tttccataat atcaagtcaa ggttaagtga ttgatttact atgaaaaaca
     3841 attcgtattt aaaaaagttc aaaggaaatt caaatataat aaaaatctgt cagaaaaaaa
     3901 ataaaaacac gtgttttttc tgattcatat ttttatgagg ttttaagttt tttgcaaaaa
     3961 tgagtgaatt cactttttga aaattaatta ccattaatta aaaaattata cacaaaaaaa
     4021 tatcgctttt acattttact ttgctatatt tgcgtcaaaa tttggttact gtgtggttta
     4081 aattttaaaa tcacaactgc tcgagaaata agtttcgacc aaattttacg gattgaaatt
     4141 taatggaata caagcttttt ggccaacaaa agttaaattc caaatatgaa gttttggaaa
     4201 ctaaaagcaa attaatatga gcaaaggatg taacaatggt attgaaaatg aatgttttgt
     4261 cggacaatac cgataaaatt aagttaaatt tgattaatca acaatacttg gtatccgatt
     4321 acaaaataaa aatttttaaa atacaagaat taagttttta tcctgattaa caaatttctc
     4381 caaaaaatta tggacaaaaa atacaatgcc cttagctttt ctaccagcaa gtttgttaca
     4441 aaattctaca gtacttcctt ttattccgct tcgcaatact tttcaaaaga aattcaggaa
     4501 gccattttca gcatatatgg ctttgtacgg ttggccgacg aaatcgttga cagttttcat
     4561 ttttccgata aggaatatct gcttcggaaa tttgaatccg attattatga tgcggtcaaa
     4621 aacggtatca gcctgaatcc catccttcat tcgtttcaaa tgactgtggg taaatatgga
     4681 atttcggatg aatatgtgca acattttctc tcaagtatga aagccgattt ggaaaaaaaa
     4741 gaatacaaaa gtcaaattga actgaatact tatgtgtatg ggtcggcaga tgttgttgga
     4801 ttaatgtgtt tgaaagtttt ttgcaatgga gatgagcaac tgttcgaccg attgatgctg
     4861 ccggctacca aacttggatc agcttttcaa aaagttaatt ttcttagaga tattaagcag
     4921 gatacgcaag agctgggcag gcattatttt ccgcaaattg ttgaagaagg gttgaccgaa
     4981 caaacgaaaa aagaactggt tgaggatatt cgaaaagatt ttgacgatgc ctacaaggga
     5041 atcgtattat tgccagccga cagcagactg ggggttttga ttgcttacaa atattatacg
     5101 gcacttttaa gaaaaattga aaaaactgct gcccgcgata ttctcaacaa aagaatcaga
     5161 atttccgatg ccggaaaaat gtttttggtt ttgtcaacgc aaataaaata ttatttaaga
     5221 gccttttatg gaaacaaata aagagaattt tgttcagttg gtggatgaaa atgatcagcc
     5281 tgtgggaatc atggaaaaat atgaagccca caaaaaagct gctttacacc gagccatttc
     5341 tgtttttatc gtgaactcca aaggcgattg gctgatacat cgcagagctt tggacaaata
     5401 ccattccaac ggactttgga cgaatgcaac ctgttcgcat ccctatcctg atgaaagtta
     5461 tctcgaagca gcaaaacgaa ggctaaaata cgaaatggga ataaattgcg aattaattcc
     5521 actttttcat tttatttatc aggaaaaagt taatgatgaa ctgacagagc atgaattgga
     5581 ccatgttttt ttcggtgtga gcgatgacgt tcccattcac aacaccggtg aggtagaaga
     5641 atggaaatac attccctatc gacaactgct ggaggatgtt gaaaatcatc ccgaaaaata
     5701 caccgtttgg ttcaaaataa tattgaaaaa agtcaaagca tacattgata cggaaaattt
     5761 aacagaaagt tcatgcagat aaacctttta cttgaaaaat tcaggcagaa atatcccgaa
     5821 aggaaagttc gacaattcat tttcaccttt tatgcggtag gaattgttgg tttggcgctg
     5881 ccggcaacgc atgttttctt tgtttatttg atcccgttgg ctttactgct ttcggctttc
     5941 atccttctcc cttttcagga agatgatttt agtatcaaaa cgggcgtatt atttactgtt
     6001 atttttgttg tttcttttac agtggaagct attggcgtaa atacggggaa aatattcgga
     6061 cagtattttt atgaaaacgg tttgggcgtt aaatgttttg gtactccgct gattattggt
     6121 ttaaactggg cattgctggt ttatctcacg gcttccattt ttcaatcgtt tcgcatcaac
     6181 aaattttttc ttgtactttt tccttctgtt ttgatggttt tgtatgatgt ggtacttgag
     6241 caggttgctc ccaaaatgca aatgtggttt tggaatgcag accgcgtccc ttttcggaat
     6301 tatttggttt ggtttctcat ggctttgatt tttcatacat tattggtaat cagcggactt
     6361 aaattaaaaa atcctttgag taaaatcgtt ttttcggctc aattcttgtt ttttcttatc
     6421 cttatgttca tcctctaaaa aggttctatg ttgaaagcca aacatcattg cctgatttat
     6481 cctttttttc gtcgatatac acttttgttg atgaaaagga attttcattc ggttaacgtt
     6541 attcgaacaa atttttctcg gaacgaaaat ttgcccgttt tgatgatagc caatcatatt
     6601 agctggtggg acgggttttg ggcaatgtat ctgaatttga aagtattcca caaaaagttt
     6661 tattttatga tgttggaaga ccaacttttg aagtttcgtt tctttcagtg gacgggcgga
     6721 ttttccataa aaaagaaaag cagggaaatg attgaatcgt tacagtatgc tgccaaaatt
     6781 ttggacaacc ccgacaattt gcttttaata tttcccgaag gaaaacttca gtcgttatac
     6841 aatcctcttc acgttttcga aaaaggaatc gaaagaattg ttgaaggaca caaaaataat
     6901 tttcaactgg tatttgtggc caattttatc gattatcttt cgcacaaaaa acccgatttg
     6961 tacatgtatg tgtccgaata tcaaggaaat gttttttcgc attccaagtt gcaagaagcc
     7021 tataatgttt tttacggcaa ctgtctcgat attcaaaaat ctaaaacaga ataagttatg
     7081 atatacttag cctgggttgt ttttggtttt actttttttc aactgtgcat tgccgggttg
     7141 aattattttt tccgtgaaaa aataccaatt tcgacaaacc ggatttccga cgaaaaaatt
     7201 tcggtgctta ttcccgctcg taatgaggaa caacatattg gaaatctttt aaatgatttg
     7261 tccggcgaag acgacaatat tctcgaaatt attgtttttg acgatctttc ttccgatcat
     7321 acagttgaaa tagtgaatca atttattggt acgaacgaaa aaatacggct gattcgttcc
     7381 acccatttgc cagcaggctg gctggggaaa aattttgcct gttattcgct tgccaacgct
     7441 gcaaagggaa attatttttt gtttctggat gcagatgttc ggatggaaaa agaaattgtt
     7501 gtttcatcgg taaaattttt caaacaaaaa tcgctgcatt tgctttccat tttcccgact
     7561 caattaatga tgacaaaagg cgaaaaaatg acggttccaa acatgaatta cattttattg
     7621 actttgctgt tgctaccttt ggtaagaaaa ttatctttcc catctatttc ggcagcaaac
     7681 ggacaattta tgctgtttga tgccgatact tataaaaaat ttcaaccaca tgaaaaattt
     7741 aaagacaagc gggtggaaga tatcgaaatt gcaaggtttt acaagaagct aaaacttaaa
     7801 attgcctgtc tcaccggaat taaacaagta agatgtcgga tgtatgaaaa ttttgctgaa
     7861 gcggtaaacg gattttctaa aaatgtaatt catttttttg gaaattcttt tttgattgct
     7921 gttttattct ggattgttac cacttttgga tggttagcag tatggctcgc attcggatta
     7981 ccgacacttg ccgtttactt gcttgctgtt gtctgtatca ggatattttt ttcgctggca
     8041 agcaatcaaa gtatattgga taatctcatt tttttcattc cccaacagtt ttctctcgga
     8101 atttttattt atcaggcgct catcaaccgc tcgaaaaatc aatatctatg gaaaggaaga
     8161 aaagtttagt tttatttttt ttgttgattt tgctgcatct gcatgttcat gcttcccaca
     8221 gggaaaatat ttacaaagcc tacattaaca acgacatgca ggcttggaaa aatacgattg
     8281 ataaaatgaa cctttcgtca ggcaatgctt ccgacgaatt tcttctggag ctggtaaatt
     8341 atgaatatgg atacattggt tggtgtattg ggaacaaaaa aactgcagaa gccaaagaat
     8401 atttaaaaaa agcagaaaac agactaatca ctctcgaaaa aaatcaattt cgcttatcaa
     8461 tgattgaagc ctataaatcg gctttttacg gatttcaaat aggattcaat aaattaaagg
     8521 cacccgtgtt gggacccaaa agcgttgaag ctgccaaatt agccattcaa tacgacccaa
     8581 acaattactt tggttatctg caaatgggta atatttacta ttattcgcct gctgcttttg
     8641 gtggttctaa agcaaaagcc attgaaagtt accgtcaggc agaaaagttg atattgtcca
     8701 ataaccaccg acttaaaaac gattggaatt atttgagcct gcttgttact ctggctcaag
     8761 cttacgaaaa cacaggcaat gacgaatcgg caaaaattta ctatcagaaa atcttaaatg
     8821 ttgaacccga ttttgcttgg gtgaaaaaca aattatatcc tgaattttta aaaaagactc
     8881 aaaaaaataa tatatgaaag aaaaagtgat catagtgggc gccggacttg gaggattggc
     8941 aacaggactt cggctggcaa aagaaggata cgaagtagaa tttttggaaa agaacaccaa
     9001 agccggaggc aggttaaatc agataaaaaa agatggtttt acattcgata cggggccaag
     9061 ttttttcagc atgtcctata tttttaagga atttgccgaa aaatgcggaa taaatctgcc
     9121 ctttgagttt attgaacttg atccccttta ttcggtaaat tttagtggtg aagaaaaaac
     9181 ttattttttg tataaggata ttcataaact tgccaaacaa ttcgatgatg tggaaccgga
     9241 ttttgaactg aaaatggaaa aatatctcca aaaatctgca cagttgttca atgacacggt
     9301 agatattgtg ataaaaagaa atttcgattc ctatctggat tattttcttg cgttgatgaa
     9361 agttaatcct gttcatttgc ccatcttgtt caaaactttc tggcaacatg tatgcgatta
     9421 ttttacctcg aagcaagcac gagaaattat ttcgctggta gctttttttc tggggaggac
     9481 gccgtttgat acaatgggaa tttacagttt actttcgcat gtcgaattcc gacacgatgg
     9541 ctattacaac gtaaaaggag gcatgtataa aattgttgaa ggaatgctcg aagaactttc
     9601 taaattggat gtcaaatttc attatcagac agaaattgtt gcatatcatg ccgaaaatcg
     9661 aaaaattgtt tcgttaaaag atgctcaagg aaatttatgg aaagccgaca agtttgtgat
     9721 caatgccgat gcggcactgt ttcgatcgaa agttatgaaa tacaaaaatt tttctgaaaa
     9781 aaaactggat aaaatggaat ggacaatggg atatttaact ttttatctgg gcttgaaatg
     9841 caaacttccg caagtaaacc atcacaatta ttttttgggg tccaactacg agcaatacgc
     9901 cggcaatatt ttacaaaatc ccgacacact cgaaaaacca tactattatg ttaacgtggt
     9961 ttcgaaatat aatcccgaat gtgctcccga aggttgtgaa agtttatttt ttgtttgtcc
    10021 cgttcccaat ttgaaataca aaaacgattg gagcgaccgc gaaaaaattg tcaacagcat
    10081 tattgacgac ttttcacaac gaatcaaaaa ggacatttct gccgaaatta tttcaaaaac
    10141 gatatttaca cctcaggaat ggcgagataa attttccttg tatcagggta gtggactggg
    10201 actgtctcat aaatttaccc aaattggagc tttccgacca aaaaattacg acgaaaaaca
    10261 cgacaatctt ttctacgtgg gagcttccac cattcccggc gccggtctgc caatggcagt
    10321 tatcagtgct gaattggtgg tggaaagaat tttaggcaag taatcgaatc gaaaagtcaa
    10381 tttttgtcaa aacatttccg gcatttctct gtcttaactc gttaataaaa cagaaaaatg
    10441 ccggataaaa aattttttca tttttcggat tttactccaa aaaaaatcag aaaatatatt
    10501 gttcgctgat agatttatgt tcctgaacgc ggcaaatagt gtcggcaaac atatcggcaa
    10561 tggaaagtat tttaactttg gaacattttg aggcatcaaa cgggatggaa tcggtaaaag
    10621 ccaattctgt cagtgccgat ttttcaactt tttcggaagc acttcccgac atcacgccat
    10681 gagaaacaat ggcacgaacc gtattggctc cctgttccat cattagattg gcagccatac
    10741 acaaagttcc tgccgtgtcc accatatcgt caacaataac cacatctttt ccctttacgt
    10801 ctccaataat tctcatttct cccaccacat tggctttttc gcgcgttttg tggcacaaca
    10861 ccattggaac gtcaaaatac ttcgaatacg aacctgcacg tttggaagca cccacatcgg
    10921 gggcagctat caccagattt ttcagacgca aagtttgaat gtatggtgca aaaattgttg
    10981 atgcatacaa atgatccacc ggtacattga aaaatccctg aatttggtcg gcatgcaaat
    11041 ccatagtaat cagtcggtcg ataccggcca cactcaacat gtcggcaata agttttgctc
    11101 ccagcgaaac gcgcggtttg tctttcctgt cctgacgtgc ccatccgaaa taagggataa
    11161 cggcaataac tttataggct gaagctcgtt ttgcggcatc aatcatcaaa agcaactcca
    11221 tcaaattatc cgaattcgga aatgtagatt gtaccaaata aacatttaat ccgcgtatcg
    11281 attcttcgta agaaacagca aattcgccat cggcaaaatg ctcaatgttc atttttccca
    11341 gcgggcatcc caaagattga cagatttttt cggctaaaga acgactgttt gttccggaaa
    11401 acactttaaa tgaggagttt gaagctgcca tgaataatta atttttgagt taaatatttt
    11461 tcgcaaaatt actcatttta ttagtaaaaa cagacttttg attcataaaa aatttttctc
    11521 ttttcaaatt atgtttcaca tgccccaata attgctacta aaagcaaaga attttatttt
    11581 tggaatttta taaattaaaa ttggtgcaaa cgtttgtata aatttttgta taattattta
    11641 cgtcaacaaa catcatttta atagaaataa atttattttt gaagttgaaa atgttaatgt
    11701 agttgaaaca cttatttcta aattatgaaa attcatttta aaatacctta ctacacccat
    11761 tggggacagc aggtttttgt ttgtggcaat cttccggagt tgggaaacaa tgttcctgaa
    11821 aaatcgtttc agttacattt tcaaaatacc gaagactggt atggagaaat agagattcac
    11881 caaaatgaac cctttgagtt ggagtaccga tatttgcttt acgaagaaaa cacaaaacgt
    11941 tttacttacg aatggattga agatcgtaaa ataaccatcg atccggcaaa aatggaacat
    12001 gttttctgtt tcgatttttg gaatgctccc ggagcggtcg aaaatgtatt ttttacggct
    12061 cctttcaaaa aagttttttt cagcgacaat ttcaatcact cacaaaaaac tcgttcaaaa
    12121 aagagtttta cccatatttt cagaatcaag gcccctttgc taaaaaatca tcaagttcct
    12181 tgtctgattg gagattgcaa agtgttgggc agttggaata cggtttcgcc cttacttttg
    12241 accagagacg gagagaattg gtggaaggca gaagtcgatc tgggtaagga aaagtcggac
    12301 gttcattata aatatggaat ttacgacacc gaaaacggac agtttttaat gtttgaaaat
    12361 ggttttgacc gaacggcacc cgtattcaat gcaaaaaaca catttgttcg tctttcggac
    12421 ggttttttgc gaatgaattc gcaatcatgg aaaggagccg gtgtttcgat tccggttttc
    12481 agtttgcgga caaaagagag ttttggtgta ggtgattttg gggatatcag gttgcttgtg
    12541 gattgggctg aaaaagccgg tttaaagttg attcaggttc ttccgctcaa cgataccatt
    12601 ggtacgcata cggatgccga tattcttcct tacgctgcca tctcggcttt tgcgttgaat
    12661 ccgctttatc tcaacttacc ctcgttggga aaactgcccg aaaatcatcc gttgcaaaaa
    12721 gaatatgagg agcgacaacc gcaattgaat gctttggaat ggattgattt tctcgaagtg
    12781 gtcaatttca aactgaagta tgtcaaagct cttttttctt ttcatcagga agagtttttg
    12841 cagaataaag attttctgac tttttttgaa aacaacaaac actggttgat tccgtatgct
    12901 gctttttgtg ttttgagaga ccggtatggt actcccgatt atcatcaatg gaaagaattt
    12961 tccactttca atgaggaaaa aattacaggg tttacttcag caaatcagcc tcattacgca
    13021 gatattgccc tgaattattt cattcagtat catttgcata tccaattaat ggaagctgcc
    13081 tcttatgctc atcagcatgg cattgtgctc aagggagata ttccaattgg cgtcaataaa
    13141 aacagtgttg aaacatgggc gcatccggaa cttttccata ttgatatgca agccggcgca
    13201 ccccccgaca tgttctccat aaaagggcaa aattgggaat tgccaactta caattgggaa
    13261 gctataaaac aaacaggatt tgactggtgg aaaaaaagat tgaaacaaat gtccaactat
    13321 ttcgatgctt tccgtatcga ccacattctc gggtttttcc gaatttggga aattcccaat
    13381 gaccagctgg agggtattat ggggcatttg tatccttcca tcccggttta tcttgatgaa
    13441 tttcaagcaa aaggaatttg gttcgattac aaccgcttct gcaaaccatt tattaacgat
    13501 caggtgctgg agcagctttt tggaactgat gcgggttggg taaaagtaaa ttgtttacaa
    13561 atagaagatg gctggatact tcgattaaag cctttttatc gcagtcagaa aaacgttcag
    13621 caattgtatg aagaaggaca attttcggaa aatataagaa acggattgtt tgatctgatt
    13681 tctaatgtgc tgttttttga agtggaaggt tcgcatggaa cgcgctttta tccgcgttgg
    13741 gggatgcatt cgctatcaac tttccaatat ctcgacgact ttaccaagca aaagttggat
    13801 gaaatttata tagattattt ttatcacagg caagatgaat tttggtttca atcgggaatg
    13861 gaaaaacttc ccgtgctcaa acaaacaacc gatatgctta tttgcggcga agatttgggc
    13921 atgatgacgc cttgcgtaac ccgagccatg aaagaattgg gaattttgag ccttgaaata
    13981 cagcgtgctc cgaaaaccaa caaaatagag tttttccatc cgagcgatgc gccttatttg
    14041 tcggttgtta ctccttccac tcacgatatg agtactattc gaggttggtg ggaagaagac
    14101 agaaacataa cgcagcgttt ttacaataca caattggggc attggggcga agcaccttat
    14161 ttttgcgaat ggtggatatg tcgggacatt ctgctccaac atgtttattc acccgctatc
    14221 tgggcaattt ttcaaattca ggatttactg ggcgtgtcgg aaaaattacg aagagaaaat
    14281 ccccatgaag aaagaattaa tgtgccgtcc gattccaaaa aaagctggcg ttatcgcttg
    14341 catttgaatt tggaagattt actgaaagaa gatgatttta atcggcaaat cagaaattac
    14401 atccggcaag caggacgata aaaaattctt gatttctatt cgcgaaatct tacagggaac
    14461 cattcttgaa gacaattttt ggttagacaa attttgtaat aaaattcatt tgttgttaag
    14521 aaacccacat gtattgttta cacaaaacga atgaatataa tattcagaca tgtgggcttc
    14581 atcagtccat taaaaaataa attatattct gatgaaaaaa tttcgtgaat catattattt
    14641 gaaatccacc agcaagcgaa gattcagctg acggccggta aggtaattgg gaacagccac
    14701 ctgatgctga taaatatctg ttacccagta gtaagaattt acattcttga aattcagaag
    14761 attgaagact tccacattga tccagatatt tttcacatgt cccagccacg atttgttcat
    14821 ccattcatcc gaaccgttta ccagcagccg cgagccacca atatccacac ggcgataagg
    14881 aggcatgcga aaagccgaac gaaattgttc gtttcgtgga gcgccaaacg gtaatccgtc
    14941 agaccaaatt aacttgagct gtaatttgta ttttggattt gagggcaaat aatcctgaaa
    15001 catcatcgaa aaagcatagc gttgctccgt cggacgaggc acccaggatg gatatatcgt
    15061 ttcggaagac aaaatattgc cttcgtcgtc gtaagtatga cgcacatagc tgtcgccaat
    15121 aatatcttcc ttcgaatcca tcagcgacag attgatccac gaatcggttc cgggaacaag
    15181 ttctccgaaa agtttgaaat cgatgccggc agtataagct ttggcatcat tttcacccga
    15241 atagcgaatg cgaacattat ccaccgaata ggaaacaacc ctgtctgcca acttaagata
    15301 agcttcggta gtgaatttga aaggtcttcc ccacgccctg aaatagtggt cgccacccaa
    15361 cacaaaatgc aacgaacgtt gtgcctttat gttggaattc aatttgatat ccacatttcc
    15421 caaagcatcg gtaagcgtat cgcgaatttc cttgtaaaaa ggagcctgat aataaagccc
    15481 cgtagcaaaa cgaaaactga aatctttctc ccaatgcgga agataggcaa cagtaaaacg
    15541 cggactgagc aaaaattcgc ggttgaaagt ccaataatga gaacgaagtc cgccggtaaa
    15601 tgaaaaagtt ccggcagtgg aagaatattt ataggtatcc tgtaaaaagg acgttgctct
    15661 aagcgttttc agcgaattgg aagcattcat gttgtaatac aaatttactt gcctgttatt
    15721 gtaaggcaaa gaatagcctg cagagtcgcg ccattcccat tcattgattt ggtcatcgat
    15781 tttttctatc tgaacgttgg caccccactt cagtaaatga ccattttttg cgtattcgcc
    15841 caaatgtgac agattggcaa ccgtagcatt cagcttgttt cgggcgtgtt cgtgataatt
    15901 tccaataccc aaagattcgc cggtttgttt atcggggtca acatccattt taacttcact
    15961 caaaatatat tcgcccgaga tgtcgtaatt ctctctttcg ttggtgtaaa atgccgaacc
    16021 gatgagactt agttttgtcg attttgaagg tctaaaattc aacgtcaacg caccgaaaga
    16081 agtttgaaaa aggtcttttt cctgtccttc aaaaaaaatg gtcagttttc gtcccatcga
    16141 ataggtgcca aatgatgttt cgcgagtctg aggaataaaa cggtagtcat tttgtgaaaa
    16201 atttcccaga aatgtcaatt cccatttggg ttttatttga taggtaagaa aagtctgata
    16261 atccacaaaa gaaggatcat attctccctg agtatccatt gttccgagca aatacttgga
    16321 agttttgtaa cgaatgccgt gcatttgtga aaaccgcttg ccggccgtac ccatataagc
    16381 cgaagccccg agcagactgg cagaggctgt tgcttcaaat gcagaaggtt ttttatagct
    16441 gatatccaaa acggaagaca ttttatcacc atattcggca ttgaaaccac ctgacgaaaa
    16501 ggatacattt tgtaccatat cgggattgat aaaactcaaa ccttcctgtt gtccggctct
    16561 aatcagcaaa gggcgataaa cttcaatccc gttaacatac acaatatttt cgtcaaaatt
    16621 tccgcctctt acattgtatt gcgaactcag ttcgttactg gaacttacgc ctgtaaaaga
    16681 aatcaacagc gattctattc cgccggccga attgggcatc aagcgatatt tggaaggatc
    16741 gaccacatca atggtagatg tctgccgtcg ctggcctatt acattcacat cttcaatttg
    16801 tttcgatagc ggaggaagtt ccacagttaa ctgtatcacc ttcaacttag gatgaacggt
    16861 ataggtaacc gtttgataac caatcataga aaaaacgagc tttatcgaat ccttgatgtc
    16921 gaccgttagt tcataatagc cattctgatt ggtagaagtg ccaatgttgg tatgttgaac
    16981 atatacattt gccaattcta tgccgcgatt gttggtatcg attacataac cgtaaatacg
    17041 cactttattt tgcgaaaaaa gcgaaatgga tagtattgaa aagaaaacaa acaaaaataa
    17101 tttacggctg tgcattgtta ttgtcttcac actaaaatta atactttttt aaacgtcgga
    17161 aacaaaagtt tattttgttg gatttttgac caaaaaacaa atagaaagca ttggtagaat
    17221 aaagaaaagc taaccgaatt tggatcattc ctctaatttg tagctttttg atcatacaaa
    17281 acagactata aaagtatgtt gcccacaaag atattaattt ataagctgaa tttaacttat
    17341 aaaggaaatt tttagtgaga caaatttcgt acttttgttc taaagttcta tgcttcattt
    17401 tgataatttt ttaggggaaa agtggcaagg taaaatatat actttacaaa gcagcaagga
    17461 ttttgttgct tgttttgcgc taatttttct ttaccttttc ttttcctaaa aagttgcatt
    17521 att
//
LOCUS       DAOSIB010000042        17444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_3473_length_17444_cov_11.228535, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000042 DAOSIB010000000
VERSION     DAOSIB010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17444
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3473_length_17444_cov_11.228535"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..1109
                     /locus_tag="PLD44_05230"
     CDS             30..1109
                     /locus_tag="PLD44_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOF98769.1"
                     /translation="MSLQLRKNAKYALLTPTSMGVRITPVNGQPVHSSQLFEMQATSA
                     ETNVASISSFLGLPVKVLTTFVKDSPIASFIKSDLHRRNIDFEGPEIPQGGPWGYRHQ
                     FNIADSGYGLRGPRVLNDRAGEVGRTLNVKDFDLERIFSKEGVKIIHLSGLIAALSPE
                     TSNFCLEIARYAKKEGTLISFDLNYRATFWKGREAELRTTFSEIASLSDILIGNEEDF
                     QLCLGIKGPAEGGKGLENAIDSFKEMIMNAQVTFPNVSVFGNTLREVISANEHLWGAI
                     VYNGKDWQVIKPRPIYVLDRIGGGDGFVGGLLYGILKEWNIEKCTQFAWASGALATTF
                     LTDYAQPADEEQIWSIWEGNARVKR"
     gene            1180..2634
                     /gene="gnd"
                     /locus_tag="PLD44_05235"
     CDS             1180..2634
                     /gene="gnd"
                     /locus_tag="PLD44_05235"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOF98770.1"
                     /translation="MKKLADIGLIGLAVMGENLVLNMESKGFTVAVYNRTVEKVDKFI
                     NGRGAGKNFIGAHSLKELVDSLERPRKVMMLVKAGQPVDDLIEQLIPLLDKGDIIIDG
                     GNSHFPDTIRRTKYVESKGLLYIGTGVSGGEEGALKGPSMMPGGSPAAWPYVKDIFQA
                     VAAKVEDGTPCCDWVGENGAGHFVKMVHNGIEYGDMQVINEAYFLMKELLGMIDDEQH
                     EVFKKWNTGKLDSYLIEITRDILAFKDEDGTPLVEKILDTAGQKGTGKWTVDAALDLG
                     IPLTLIGESVFSRCLSAQKDLRVEASKAISGPKKAFKGDRQQFINDLENAIYASKIVS
                     YAQGYDLMMQAAKEYGWNLNYGGIALMWRGGCIIRSVFLGDIKKAFDQNPNLRHLLLD
                     PFFKEQVESAQEGWRRVCAVAMENGIPVPAMASALSYFDGFRTERLPANLLQAQRDYF
                     GAHTYERIDRPRGEFFHTNWTGRGGDTASTTYNV"
     gene            2749..4035
                     /locus_tag="PLD44_05240"
     CDS             2749..4035
                     /locus_tag="PLD44_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOF98771.1"
                     /translation="MIYFEKGSTTTNLTSEDLKNGLYEALVKLGKRQKVLAIPPDFTR
                     YPSHAGELTEFAWQYYGEKLTDVLPALGTHTGMTDEQIATMFGQVPKAIFRVHDWRHD
                     VVTLGYVPGSYVSEVTEGALSFDWPAQVNKLLVEGNFDLILSIGQVVPHEVVGMANYN
                     KNIFVGTGGQEGINKSHFIGAVYGMEKMMGRANTPVRKVFNYASEHFAAHLPIVYVQT
                     VVALNKETGKLQTYGLFIGDDFEVFDKAAGLSMQVNFEMVEKPLNKVLVWLDPAEFKS
                     TWLGNKAIYRTRMAIADGGELIVLAPALREFGEDKEIDRLIRKYGYFGTPKTLKATNE
                     NEELQQNLSAAAHLIHGSSEGRFTITYCPGKNPENLTKEEIESVGFRWGDIDEITAKY
                     HVSTLKEGWNVMPDGEEIFYISNPALGLWAHKDRFR"
     gene            4661..5125
                     /locus_tag="PLD44_05245"
     CDS             4661..5125
                     /locus_tag="PLD44_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOF98772.1"
                     /translation="MALPKVFRSGSNRTPSLPSLIDRFFDTDLLDWDAFNFAGENMTL
                     PAVNVKETKDEYCIEVAAPGLNKEDFKVSYDDGMLTISSEKQQEKEEKEGEKVTRREF
                     SYESFQRSFTVPEEVVNADKISAKYENGILYVTLPKHEKAKKEKNVKEIKIS"
     gene            5640..6887
                     /locus_tag="PLD44_05250"
     CDS             5640..6887
                     /locus_tag="PLD44_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98773.1"
                     /translation="MYGMNDFCKTPSLLNKAFGNNVELLPGVNELFELELAFMEYNCL
                     PSNQLLERTAFIKSLNNHFSKHYLLYSNYSEKINTERTSATQAYFEEGKFSTGYATHG
                     LFPYRGKFHPQLIKGLMNILNIQKGETVLDPMAGSGTTNIESALMGINSIAIDVSPFC
                     QFMIKTKYEALTIELKSLENTKFDSRKIFELFTNGNVLERINKIEDNNKRKIYDLAFL
                     AFLDALGYSKRVIKSNHQQLFDKVLPRYIETVKSFLSNPYFEQDKIGSLKIVSDSDAL
                     NMKIEKDSIDYVITSPPYSFAIDYAENDRDQLEFLGYDADELKNKLIGLKGKTKSQKL
                     QNYFSDMDTFCAQVSRLLKQGKYFVMIIGSNTNQTGGVRLEETVINSALKYDMPLIKS
                     ILKPIKGMRNTMKDEYVLIFEKQ"
     gene            6890..7873
                     /locus_tag="PLD44_05255"
     CDS             6890..7873
                     /locus_tag="PLD44_05255"
                     /EC_number="3.1.21.4"
                     /inference="COORDINATES: protein motif:HMM:NF016443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DpnII family type II restriction endonuclease"
                     /protein_id="HOF98774.1"
                     /translation="MSKSVEDKIQTVIRKNTFYFFDPKFEEIYEGRINSLKETLLIVK
                     NKVEIEGLKKEIFEWLLFEKENGLRALLALTGFSNEYLKRITTIIRIVDDPELNKLVY
                     KDKWYNEKNPDNIQEWSDSTIQKLIRGNEYFRKGIVNIFFEGGSIPFLTETIPLFELK
                     KLSISKLKFEIPELIDTLIRYKEKGSYSGMKGNNPETLIANILDKLGITYETGDLTEL
                     IDNAPNNKRTMDFIIPNKKNPAIIIESSFLATTSSGQGDKSKTEISIDTLIKSHYPKA
                     IFVGFVDGIGWYVRKGDLRRMVTAYEDVFTFHKDELERFEKLLIERISNVR"
     gene            7863..8918
                     /locus_tag="PLD44_05260"
     CDS             7863..8918
                     /locus_tag="PLD44_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOF98775.1"
                     /translation="MLDKYLNKITQGDCLELFKDIPDNSVDVTFADPPFNLKKNYSSY
                     KDSREFQEYLDWCEKWISEMVRVTKPTGSILVHNIPKWLTYYATFLNKHAHFKHWIAW
                     DAPTAPMGKSLQPAHYGILFYTKEQKGSKIYELRYPHKRDRKQGYLWKDYGGKKDLLH
                     PFGPLVSDVWTDIHRIKHNSKRDPHPCQLPIHLMDRLILLTTDEGDIVLDPFSGTGTT
                     AISAKRLGRQFIGFELDSKYVEISEEKLEHTLSNFKIGESWVSFYLNEIITIKDKDWE
                     NLSKYFLIPNPIKNIDFHRIELLNKDLIPKEIENFQNGKTNQQDKFYKIESKKTTEIE
                     NLDSAIILTKNKINQAL"
     gene            9047..9700
                     /gene="rpe"
                     /locus_tag="PLD44_05265"
     CDS             9047..9700
                     /gene="rpe"
                     /locus_tag="PLD44_05265"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOF98776.1"
                     /translation="MNKKIISPSILAADFSNLQAVCELINQSEAEWIHLDVMDGVFVN
                     NISFGFPVLEAVRKHCRKMMDVHIMIVHPEKYVTRFAKAGAEMLTFHFEAAENPQKVI
                     DQIHEEGMKAGITINPDVPVKVLEPFLNQVDMVLLMSVFAGYGGQPFIEETYERMDEL
                     KKMMELKKATCLLEVDGGVNLENAPKLFSHGANVLVAGSSIFKAENPLEIIQQMKNC"
     gene            9792..11864
                     /locus_tag="PLD44_05270"
     CDS             9792..11864
                     /locus_tag="PLD44_05270"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOF98777.1"
                     /translation="MNFLQRTPFIRLLLAYICGIVVYHYAELFPATLWSLFFLSIALL
                     LIPFFIRETRLQFNFRWLFGTGVFLFLFITGYIFSFERDKASKFYPLNQKGIYVVELT
                     SAPVEKAKSVFYTAEIKKFLHNRHWESGHGKAYIYLQKDSLSGNLVFGDRLMLETEFK
                     SPEKLLNPDGFDYATYLKRQGVGATAYIASQHWQKIGHNQAFSVRRMADQCRNYLLGI
                     YRKFNIQGNEFGVLAALTLGYTEDLQPDLRKSFSASGATHILSVSGLHVGTVYAVLLF
                     IFGFLNHNQRQKISKIIIITLCMWGYAFITGLSPSVVRATLMFTFVALANCLERKSQI
                     YNTIFMSAFFMLLVNPNFLFDIGFQLSYSAVLSIIFFQPPLTKLLPTNHRFLKWGRDL
                     LLVSVAAQIGTAPFTLYYFQQFPNYFLLTNLLVLPLSTLVIYLAMGLLAVSFIPYLST
                     AMAFLLKISVGLLNFCIVQIEHFPYSLSYISLDIKQTILIFMVIFSLSAYYYNKKFVP
                     LALGLISLLLVCVVDLQIKYNTLRNQKIIVFAGQKSTHVNFIDHGKNYVYTTDSVELE
                     KIASNYWRKNKLQNPEYLSNSNWFENGFASFYGIKILILTDEQLKRKVTETPLNIDLL
                     IIGNHLKPRMDQILSCVQPQKIIVDKSISKWYTNDIRQQCEKHGIEFYSVADQGAYIL
                     DIPNKKSL"
     gene            12174..14216
                     /locus_tag="PLD44_05275"
     CDS             12174..14216
                     /locus_tag="PLD44_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOF98778.1"
                     /translation="MKKSLLPAFMLAFVTCCGIFAQNSPSKFGNVGKEELEQNVCPID
                     SNAHAYFIFNCGLSRFTYPQTHVREDDAGENQGFVLETTKHFRIKILDESAFDWANIE
                     IPLYVSGKKKEKLSSFKASTFNINNGKVEETKFNKGNLIFESTSENWETAKAPLPNVK
                     KGSIIEVVYTVTSVFFYNLDKWNFQYSIPVLFSDFEVEIPEYFNFHQNIAGYYAIDRK
                     TERKNESLDLTYHWQDFNGRYSNTQRINFYVDISEYTGRNIPAFKEDSYLKTPKNYLS
                     AVEFELAYTKFPNSTLKYYTTSWENVIEQYLKYNDLENSIKVGFLDDIAKEINNKAKN
                     PYSKMELAFYNIANHYKWNEKNSIFTSKGFKDSYTNATGNCADINLCLVGLLQKLGLE
                     AYPVILSTQNNGFIKQTHPSLSNFNYLVALAKIDGNDYLMDATEPLSIINQLPVRCLN
                     DKGLIVKKGQANWVDMVKDASYKSQIILTYELDENLEAKGTYSSILSNYAALEIRKNI
                     KSYTSNDEYFKEIAKNNPGIETNEYQIEGLDSLNMPVQMKFDFSYNNAVEQAGDLIML
                     KPLLFEAMKQSPFKLSERTYPVEFNYQNKDKTTCQIKIPNGYAVESLPSSINLTLPDK
                     SAQFLFFTSIKDDYILIVSNYTRNKLLYLPEEYEVLKEFFRNMVVKQNEKIVLKKL"
     gene            14288..16111
                     /locus_tag="PLD44_05280"
     CDS             14288..16111
                     /locus_tag="PLD44_05280"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOF98779.1"
                     /translation="MANIPDSLKENADAIIRYSNVSYEIKENKLIKRKSFAITLMNDN
                     AKENAYLVIPYDSYTNIKKIEGYIYNANGIKTEKLSKENIKDYAAYPSFTLFSDNRIK
                     VVGFSGKNYPVTIEYIYELESKGLLMPESWVPINDYRRSLEKAQLQIIVPDSIIQYKY
                     QNIRPTFTKETVNNNIIYSWKINGIKAKESEPFSLPFQLIMPKIFVNATKTKYFEYPV
                     EFNDWKSYGKMVFNFLTGREILLTKSVEEINSLCKNANTELEKVKIVYAYVQNKVRYV
                     NVTKGIGGFQPLPAAEVDKYGYGDCKALSNYTRALLNAVGIKSYYAEIGNGNNKRIQF
                     PDFASVDQTNHVILFVPLKSDSLWLECTSNTIPFGYIGSGNCNRQALVVTEEGGKLID
                     TPSYNEFDNLLSISGEIFVEETGNAHLKIKKTYSNELFDEVMPFLSLSKQETEKYINK
                     SIPINGFHLTGYSFINKSSYKNIKGELAFEGDILQYAELMGKRIALKPNIISRSYFGL
                     NKKRNNNVYFSQSFTQTDTIAIHVPSGYKLEFEYPEISFSCPAGYYSTKISQKKADSF
                     VMIRKLTIKKGIYGKENYPEIYDFFKNSALADNKSCIFTKL"
     gene            16262..17290
                     /locus_tag="PLD44_05285"
     CDS             16262..17290
                     /locus_tag="PLD44_05285"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOF98780.1"
                     /translation="MISKIIAEDLINKVYSSLQDAEKIAIVVHVNPDGDAMGAALGLW
                     HYLMTIEKEPTVISPTAYPNFLSWMPGANEVLIYENDKEKADNVLASAELIFTLDFNN
                     LHRLEEMADAVASSPAPKILVDHHLHPESFAKITISYPEISSTSEMIFRLICRMGDFA
                     NINLACAECIYTGMMTDTGSFTYNSNDPEIYIIISELIKIGIDKDEIYRKVFNTYSEN
                     RLRLMGFCLYEKMQTFPEFRTAIISLTQEELSQFNYVNGDAEGFVNIPLSVKGIDFSV
                     FLREEKNRIKISLRSQGKFPANKVAADLFGGGGHLNAAGAESYASMQETIHTLLNALP
                     DYQVFFEE"
BASE COUNT         5805 a         3096 c         3330 g         5213 t
ORIGIN      
        1 cattaattta ataaaaaaat aatattgata tgtcgcttca attacggaaa aatgcaaaat
       61 atgccctgct gacacctacg agcatgggtg tacgtattac tcctgtgaat ggacaacctg
      121 ttcatagcag tcaattgttc gaaatgcagg ctaccagtgc cgaaaccaat gtggcaagta
      181 tttcctcatt tttaggtctt ccggtcaaag tgttgactac tttcgtaaag gacagtccga
      241 ttgcttcgtt tatcaagtcc gacttgcatc gtcggaacat cgattttgaa ggaccggaaa
      301 tccctcaagg tggtccttgg ggctatcgtc atcagttcaa cattgccgac agcggttatg
      361 gattgcgagg gccgcgtgtg ctcaacgacc gtgccggtga agtgggacga actttgaatg
      421 taaaagattt tgatctggaa cgaatttttt ccaaagaagg agtgaaaata attcatttgt
      481 cgggattgat tgctgcgctt tcgccggaaa cgagcaattt ttgtcttgaa attgcacgct
      541 atgcaaaaaa agaaggaacg ctcatttctt ttgatttgaa ttatcgggct actttttgga
      601 aaggacgcga agccgaatta aggacaactt tctccgaaat tgcttctttg tcggatattt
      661 taatcgggaa tgaagaagat ttccaacttt gtctgggcat aaaaggaccg gccgaaggag
      721 gcaaagggtt ggaaaatgcc attgattctt ttaaggaaat gattatgaat gcacaggtaa
      781 cttttccgaa tgtgagcgtt ttcggaaata cgcttcgtga agtcatcagt gcaaatgaac
      841 atttgtgggg tgctattgtt tataatggaa aagattggca ggtcatcaaa ccacgaccca
      901 tttacgtgct cgaccggatt ggcggtggtg atggattcgt aggcggttta ctttacggga
      961 ttctgaaaga gtggaatatc gaaaaatgta cccaatttgc gtgggcaagc ggtgcattgg
     1021 caaccacttt ccttaccgac tatgctcaac cggccgatga agaacaaatt tggagtattt
     1081 gggaaggaaa tgcccgcgta aaaagataaa agctatagaa cttcgacaga agaaacctct
     1141 ttttataaaa cagaatattg aaataaaaac agaaaaaata tgaaaaaatt ggcagatatt
     1201 ggcttgatag gattggccgt catgggtgag aatttggtac tcaacatgga aagtaaaggc
     1261 ttcactgtag ccgtttataa tcgtacggtc gaaaaggtgg ataaatttat caacggacgt
     1321 ggagcaggga aaaattttat cggtgctcat tcgttaaaag aattggtcga ttcgctggaa
     1381 aggccgcgta aagtaatgat gctggtgaaa gccggacaac ccgtggatga tttgattgaa
     1441 cagcttattc cgctgctcga taaaggcgat attatcattg acggcggaaa cagtcatttc
     1501 cctgatacca tacgtcgtac taagtatgtc gaaagcaaag gcttgcttta tattggtacc
     1561 ggcgtttccg gaggcgaaga aggagcatta aaaggtccat ccatgatgcc cggaggttct
     1621 cctgcagctt ggccttatgt gaaagatatt tttcaggctg tggccgcaaa agtggaagat
     1681 ggaacgccat gctgcgattg ggttggcgaa aatggtgccg gacattttgt aaaaatggtt
     1741 cacaacggca ttgaatatgg cgacatgcaa gtaatcaacg aagcttactt tttaatgaaa
     1801 gaattgcttg gcatgatcga tgacgagcaa catgaagttt tcaaaaaatg gaacaccggc
     1861 aagttggatt cctatctgat tgaaataacg agagacattc tcgcatttaa agatgaagac
     1921 ggaacgccgc tggtggaaaa aatcctcgac acggccggcc aaaaaggaac agggaaatgg
     1981 acagtggatg cagcgcttga tttgggcatt ccgctgactc tgattggtga atcggttttt
     2041 tctcgttgct tgtcggcaca gaaagattta cgcgtggaag cttcaaaagc catttcagga
     2101 ccgaagaaag catttaaagg cgaccgccag caatttataa acgatctgga gaatgccatt
     2161 tatgcttcca aaatagtttc gtatgcacaa ggttacgatt tgatgatgca agctgccaaa
     2221 gagtacggct ggaatctgaa ttacggcggc attgcgctga tgtggagagg cggctgtatt
     2281 attcgttccg tatttttggg agatatcaaa aaagcatttg accagaatcc gaatcttcgt
     2341 catttgctgc tcgatccatt cttcaaagaa caggttgaat cggctcagga aggttggcga
     2401 agagtttgtg ccgttgcaat ggaaaacgga attccggttc cggctatggc ttctgctctt
     2461 tcctatttcg acggattccg caccgaacga ctaccggcaa atcttttaca ggcacaacgc
     2521 gattatttcg gagcacatac ctacgaaaga attgaccgtc cgagaggtga atttttccac
     2581 accaactgga caggaagagg cggcgacact gcttcgacta catacaatgt ttaatgaata
     2641 atgttttttg aatttgatcc attctgacag atgccctcca aaagctctgt cagaattgtt
     2701 tttacagtat gtttctacat cccagaattt tcattatcaa caaccattat gatttatttt
     2761 gaaaaaggtt caaccacgac gaaccttact tccgaagatt taaaaaacgg actttacgaa
     2821 gctttggtaa agttaggcaa gcgacaaaag gtgctggcca ttccgcccga tttcactcgc
     2881 tatccaagtc atgccggaga gctgacggaa tttgcatggc aatattacgg cgaaaagctc
     2941 acggacgttt tgccggcatt gggcacgcat accggcatga cggacgaaca aatcgcgacc
     3001 atgttcggtc aagtgccgaa agcgattttt cgtgttcacg actggcggca tgatgtcgta
     3061 acgctgggtt atgtgcccgg atcgtatgtt tcggaagtaa ccgaaggagc gttaagtttc
     3121 gactggccgg ctcaggtgaa taagctgctg gtggaaggta atttcgattt gattctttcc
     3181 atcggacagg ttgtgccgca cgaagtggta gggatggcca attacaacaa aaatatcttt
     3241 gtgggaacag gcggacagga gggaatcaac aaaagtcatt ttatcggagc ggtttacggc
     3301 atggaaaaaa tgatgggacg agccaatacg cccgttcgga aagtattcaa ctatgcttcg
     3361 gagcattttg cagctcatct gccgattgtt tatgtgcaaa ctgttgtcgc tttgaacaaa
     3421 gaaaccggga aactgcagac ttacgggctt ttcattggcg acgatttcga ggtttttgac
     3481 aaggcagcgg gtttatccat gcaagtaaac tttgaaatgg tggaaaaacc tttgaataaa
     3541 gttctggttt ggctcgatcc ggctgaattc aagagcacat ggctcgggaa taaggcgatt
     3601 taccgaaccc gcatggctat tgccgacgga ggtgaactga ttgtgctggc accggcattg
     3661 agggaatttg gcgaagacaa ggaaattgac cgactgattc gaaaatacgg ttattttgga
     3721 acacccaaaa cattgaaagc aaccaatgaa aatgaagaat tgcaacaaaa cctgagtgcg
     3781 gctgcccatt taatacatgg ttcttctgaa ggacgattta ccattactta ttgtccgggc
     3841 aaaaatccag aaaatctaac caaagaagaa atagaaagcg ttggttttcg ctggggtgat
     3901 attgacgaaa ttacagcgaa atatcacgtt tctactctga aagaaggctg gaacgtgatg
     3961 cccgacggag aagaaatttt ctacatttcc aatcctgctt tgggactttg ggctcacaaa
     4021 gacaggtttc ggtaattcgt ctgtttgaat tttcggcgtt tctgctcgag agagttttca
     4081 ttaaaattat tacaggaaat cggtcattgg aaaacgattg aatttcgttg atcgacgact
     4141 taacaaggcg aacttgaaat cacagaaaat tcatttacaa aattgctttt acgtacttta
     4201 ttttatctat ccgcaacctt ttactcagat gttaaagaaa gcttcaaaaa agtcgtcagt
     4261 caatataaaa atttgtagaa ggttgtattt caatttgaat ggtagaaaaa atatttgaca
     4321 atttttcctt gttggataat ttctaaagtg taattggttt tattaattga aaaccttctc
     4381 aaactaaaaa tactcagcga ataatttagt cctgtcatat tggcagtttt ttatacattt
     4441 cgacagatat tattcaatta ttacgcttta aaggtgcttt gtttttttaa tccataaatt
     4501 ctattcgtca attagttctg tttttcaatg gtatagatat gatgtcaata atttttattt
     4561 ttgctggtac gcgttttgaa caataaaata tgaaatttat caaaaaagat ttcaaaaagc
     4621 aattgatggt ttaacttata aaatatagga ggtgttaatt atggcattac caaaagtttt
     4681 cagatctgga agtaatcgaa ctccttcact tccatcgttg attgacagat tttttgatac
     4741 cgacttactg gattgggacg cattcaactt tgcaggtgaa aatatgacgc tgcctgcagt
     4801 gaatgtaaaa gaaacaaaag atgaatattg catagaggtt gctgctcccg gtttgaacaa
     4861 agaagatttc aaagtaagtt atgacgacgg gatgttgacc atatcttccg aaaaacagca
     4921 ggaaaaagaa gaaaaagaag gagaaaaggt tactcgtcgc gagttcagtt atgaatcgtt
     4981 ccagcgtagt tttaccgttc ctgaagaagt tgtgaatgct gacaagattt ctgcaaaata
     5041 tgaaaacggt attttgtatg taaccttgcc aaaacatgaa aaagccaaga aagaaaaaaa
     5101 tgtaaaggaa atcaagattt cataattttc tacttttaat ccaataaaaa caggaggatt
     5161 ttcagggaag gaagatcttc ctgttttttt tttgacaact taaaaaaatt cacttagaac
     5221 cttacaaaaa aattaaattc attgaagaag ttccgctcaa cataaatcaa ataaatttga
     5281 ttttgttttc gcttaaacgc tgctttcaag actgctcccg cttcactcga aaagctgaaa
     5341 tcgcccaaac tcattctgac gaaatacgtc ggaacctaaa caaggacgat ttttaaacgc
     5401 ttttctcact tatttttcgg ctcaccggac aaagtaaacc cgaagtgttt ggcttatatg
     5461 tcctgtttta cagatatata aaacaaatgt ttcaattttt tcatgtgcta aaattctact
     5521 taaccaacgc tgctattata aaaatatttg tatttttgta gttaaacatt tatttcatcg
     5581 aaacaataat acactaaaaa attcagttca caattaaacg gcttctctga atttattcta
     5641 tgtatggtat gaatgatttt tgcaaaactc cttcacttct aaacaaagct tttggcaata
     5701 atgtagaact tttgcccggt gtcaatgagc tttttgaatt agaattggca tttatggaat
     5761 acaattgttt gccaagtaat cagttactgg aaaggactgc tttcataaaa tccctcaaca
     5821 atcatttttc aaaacattat ttgttatact caaattattc cgaaaaaata aatactgaaa
     5881 gaacttcagc aacccaagct tattttgaag aaggtaaatt ctcaaccgga tatgctactc
     5941 acggactatt tccttatcgg ggaaaatttc atcctcagtt gattaaagga ttgatgaaca
     6001 ttctcaatat tcagaaaggc gaaactgttt tagatccaat ggcaggcagc ggcactacta
     6061 atatcgaatc agccttgatg ggaataaatt caatagcaat cgacgttagc ccattttgtc
     6121 aatttatgat aaaaacaaaa tatgaggcat taaccatcga attaaagtca ttggaaaaca
     6181 caaaatttga cagtagaaaa atatttgagc tttttacgaa tgggaacgtt ttggaaagga
     6241 taaacaagat agaagataat aataaaagaa aaatatacga ccttgctttt ttggcttttt
     6301 tagatgcgtt gggttactct aaaagagtaa taaaatcaaa tcatcagcaa ttgttcgata
     6361 aagtattgcc acgatatatt gaaacggtga aatcattttt atcaaacccg tattttgaac
     6421 aagataagat tggcagtttg aaaattgtat ccgattcaga tgctttaaat atgaaaattg
     6481 aaaaggattc aatcgattat gtaatcactt cccctcccta ttctttcgca attgattatg
     6541 ccgaaaatga cagagaccaa ttggaatttt taggttatga cgccgatgaa ctaaaaaata
     6601 aattgattgg gttgaaagga aaaaccaaaa gccaaaaact tcaaaactat ttttctgata
     6661 tggatacttt ttgtgcacaa gtttctcgcc tattaaaaca agggaaatat tttgttatga
     6721 taatcgggtc aaacacaaat caaaccggcg gagtaagact ggaagaaacg gttataaatt
     6781 ctgctttgaa atatgatatg cctttgataa aaagtatatt aaaacccata aaaggaatga
     6841 gaaacaccat gaaagatgaa tatgtactaa tttttgaaaa acaataaaca tgagtaaaag
     6901 tgttgaagat aaaattcaaa ccgtgatacg gaaaaatact ttttattttt ttgatcccaa
     6961 attcgaagaa atttatgagg gccgaatcaa ttcattgaag gaaacacttt taatcgttaa
     7021 aaacaaagtt gaaattgaag gattgaagaa agaaattttc gagtggcttt tatttgaaaa
     7081 ggaaaatgga ttgagagctt tattggcttt aaccggattt tcgaacgaat acttaaaacg
     7141 aattacgacc attattcgaa ttgtagatga tccggaatta aacaaactcg tttacaaaga
     7201 taaatggtat aatgaaaaaa atcctgacaa tattcaagaa tggtctgaca gtaccattca
     7261 gaagctcata aggggaaatg aatattttag aaaaggaatt gtaaacatat tctttgaagg
     7321 cggttctatt ccctttttga cagaaacgat tcctcttttc gaattgaaaa aattgagtat
     7381 ttctaaactg aaatttgaaa ttcccgaatt aattgacact ttaatccgtt ataaggaaaa
     7441 aggaagttat tcaggtatga aaggcaataa tcccgaaaca ttaattgcaa atatcctcga
     7501 caaattggga attacttacg aaaccggcga tttgacagag ttaatcgata atgcgccgaa
     7561 caacaaaaga acgatggatt ttattatccc gaacaaaaag aatccggcaa tcattatcga
     7621 aagttcattc ttagccacca cttcgtccgg tcaaggtgat aaatccaaaa cagaaatttc
     7681 tattgacaca ttaattaagt ctcattatcc aaaagcaatt tttgtcggtt ttgtggatgg
     7741 tattggatgg tacgtcagaa aaggtgattt acgaagaatg gtcacggctt atgaggatgt
     7801 ttttactttt cacaaggatg aattggaaag atttgaaaaa ctattaattg aaagaattag
     7861 caatgttaga taaatactta aataaaatta ctcaaggtga ttgtctcgaa ttatttaaag
     7921 atataccgga taattctgta gatgttactt ttgccgatcc gccattcaat ttgaaaaaaa
     7981 actattcgag ttataaagat agtcgcgaat ttcaagagta tttggattgg tgcgagaaat
     8041 ggatttcaga aatggtcaga gtaacaaagc cgacaggttc aattttagtt cataatattc
     8101 ccaaatggct gacatattat gccacatttc taaacaaaca tgcccatttc aagcattgga
     8161 tcgcatggga tgcccctact gcgccaatgg gaaaatcgct tcaaccggct cattatggga
     8221 tactttttta tacgaaagaa caaaaaggtt ctaaaatcta tgaattaaga tatcctcata
     8281 aacgagacag aaaacaaggt tatttgtgga aagactatgg aggcaaaaaa gatttattgc
     8341 atccattcgg accattagtt tctgacgtct ggacagacat tcatcgaatt aaacataatt
     8401 cgaaaagaga cccgcatccc tgtcaattac ccattcatct gatggataga ttaattcttt
     8461 tgacaaccga tgaaggagat attgttttgg atccgttttc aggaaccggc acaactgcta
     8521 tctcggcaaa acgactggga cggcaattta tcggttttga attggatagt aaatatgtag
     8581 aaatatcgga agaaaagctt gagcatactt tatctaattt taaaattggt gaaagttggg
     8641 ttagttttta tttaaacgaa atcattacaa taaaagataa agattgggaa aatctttcaa
     8701 aatatttttt gatacccaac ccaataaaaa acatcgattt tcacagaatc gaattactca
     8761 ataaggactt gatacctaaa gaaatagaaa attttcaaaa tggaaaaaca aatcaacaag
     8821 ataaatttta taaaattgaa agcaaaaaaa ctactgaaat agaaaattta gactctgcta
     8881 taattctgac aaaaaataaa atcaaccaag ccttataatt ttgataatat tttaacttat
     8941 ttttttaacc aaaattttca tattttttgg aacttgttaa attacatttt ttgttatttt
     9001 tgcaaaagca cagctgttaa ttttctaaat ttatacggaa catcgcatga acaaaaaaat
     9061 catttctcct tcaattttgg cggccgactt cagcaatttg caggctgtct gcgaactgat
     9121 caaccaaagt gaagccgaat ggattcactt ggacgtgatg gacggagttt ttgtcaacaa
     9181 catttctttt ggttttcctg tattggaagc tgtacgcaaa cactgtcgaa aaatgatgga
     9241 tgttcatatt atgatcgttc atcccgaaaa atacgtaaca cgctttgcaa aagcaggcgc
     9301 cgagatgttg acttttcatt ttgaggctgc cgaaaatcca caaaaagtaa ttgaccaaat
     9361 tcatgaggaa gggatgaaag ccggcatcac catcaatcct gatgtgcctg tcaaggtgct
     9421 tgagcctttt ctcaatcaag tggatatggt tttgttgatg agtgtttttg cgggttatgg
     9481 cggtcagccg tttattgaag aaacttacga acgcatggat gagctgaaga aaatgatgga
     9541 actcaaaaag gctacttgct tgctggaagt tgacggaggt gtcaatctgg aaaatgcccc
     9601 caagcttttc tctcatggag caaatgtgct ggtggcagga agttcgatat tcaaagccga
     9661 aaatcctttg gaaataatac agcagatgaa aaattgttga aaagtctttc tcgcctttgg
     9721 cggcacgatt ttaaaattga aaaattttcc ggccggaggg tttcaaataa aacatttttc
     9781 aggtattttg catgaacttt ttacagcgaa cccctttcat tcggttactg cttgcctaca
     9841 tctgcggcat tgtagtgtac cattatgcgg aattgtttcc cgcaacgcta tggtctctat
     9901 tttttctttc aatcgcatta ctgctgattc ctttttttat ccgcgaaacg cggcttcaat
     9961 tcaatttcag atggctattc ggcactggcg tattcctttt tctttttatt acgggttata
    10021 tttttagttt tgaacgcgat aaagcatcga agttttatcc cctgaaccaa aaagggattt
    10081 atgtggttga attgacttcg gctccggtag aaaaagcaaa atcggttttt tatacggccg
    10141 agataaaaaa atttcttcat aaccgacatt gggaatcggg acatggcaaa gcttacattt
    10201 atcttcaaaa agacagtttg tcaggcaatt tggtttttgg cgaccgtctg atgcttgaga
    10261 ctgaatttaa aagccctgaa aagttgctga atcctgatgg ttttgattat gctacttatc
    10321 ttaagagaca gggagtggga gcaactgcct atattgcttc acaacactgg caaaaaattg
    10381 gtcataatca agctttttcg gttcgtcgca tggcagatca atgcaggaat tacctgctgg
    10441 gaatttacag aaaatttaat attcaaggaa acgaatttgg ggttttggca gctttgacgc
    10501 tgggttatac tgaagattta caacccgatt tacggaaaag tttcagcgct tcgggagcaa
    10561 cgcatattct ttcggtaagt ggacttcatg tgggaacagt ttatgccgtt ttgcttttta
    10621 ttttcggatt tctgaatcat aatcagcggc aaaaaatttc caaaataatt attatcactt
    10681 tatgtatgtg gggctatgcc tttattacgg gtttgtctcc ttctgtagtg cgggcaacac
    10741 tcatgttcac attcgttgct ttagcaaact gtctggaaag aaaatcgcag atttacaaca
    10801 cgattttcat gtcagccttt ttcatgctgc tggtgaatcc gaattttctt ttcgatattg
    10861 gttttcagct cagctattcg gccgttctga gcattatttt ctttcaaccg cccctgacta
    10921 aattgttacc taccaatcat cgatttttaa aatggggaag agatttgctt ctcgtttcag
    10981 tagcagctca aattggaacc gctcctttca cactgtatta ttttcaacag ttccccaatt
    11041 attttctgtt gaccaatcta ttggttttac cgctctccac actcgtaata tatttggcaa
    11101 tgggtttgct ggcagtttca tttatcccct atttgtccac agctatggct tttttgctga
    11161 aaataagtgt tgggcttctc aatttttgta ttgttcaaat tgaacatttc ccctactctc
    11221 tttcttatat ttctctggat ataaagcaaa ccattctaat tttcatggta attttcagtc
    11281 tatcggcata ctattacaac aagaaatttg tacccctcgc gttgggactc atttcgctgc
    11341 tgctggtttg tgtagtggat ttacaaataa aatacaatac tctacggaat cagaaaataa
    11401 ttgtttttgc cggacagaaa tcaacacatg tcaactttat cgaccacgga aaaaattatg
    11461 tctatactac agattcggta gaattggaaa aaattgcatc caactactgg cggaaaaaca
    11521 aattacaaaa tcctgaatac ctttcaaaca gcaactggtt tgaaaatggt tttgcttctt
    11581 tctacggcat aaagattctg attttaacag acgaacaact gaaaagaaaa gttacagaaa
    11641 ctccattaaa cattgattta ctgattattg gcaatcatct gaaaccccga atggatcaaa
    11701 ttctttcttg tgtgcaacca caaaaaataa ttgtcgacaa aagcatctcg aagtggtata
    11761 ccaacgacat ccgacaacaa tgcgagaaac atgggattga gttttattct gtagctgatc
    11821 aaggtgccta tatcttggat attcccaaca aaaaaagtct ttaaaacggc aattcagcca
    11881 aattaactca ccaacctttc aaataatttc gcccttatgc gtccccaaaa attagaaata
    11941 tctctgagga aaaaattttt tgttgctatt tctaaaaatg tgtaggaaaa tttaaaatat
    12001 aattttaact ttgctctgta ttaaaatttt gaacttattt taactttttt gatggaagag
    12061 tattcatcct gtgtattcca tcaatgctca gagaaatttt ttctctgggg gataattatt
    12121 ttccaatttt tccataaaaa cgacattgaa ttatatcaat ttacaataat atcatgaaaa
    12181 aaagtttatt acctgccttc atgttggcat tcgttacttg ctgcggtata tttgcacaaa
    12241 attctccttc aaaatttggc aatgttggca aagaagaact ggaacaaaat gtttgtccca
    12301 ttgactcaaa tgcccatgcg tattttattt tcaattgtgg gctttctcgt tttacctatc
    12361 cacagaccca cgtgagagaa gatgatgccg gcgaaaatca gggctttgtt ttggaaacca
    12421 ccaaacactt cagaataaaa attctcgacg aatctgcctt cgactgggca aacatagaaa
    12481 taccccttta tgtatcaggc aaaaaaaaag aaaaactttc ttctttcaaa gcttctactt
    12541 ttaatataaa taacggaaaa gttgaagaaa caaaattcaa caagggaaat ctgatttttg
    12601 aaagcacttc agagaattgg gaaacagcca aagctccact tccaaacgta aaaaaaggaa
    12661 gtattattga agttgtttat acggttactt ccgtattttt ctataatctc gacaaatgga
    12721 attttcagta ttcaattccc gtccttttct ctgatttcga agttgagatt ccggaatatt
    12781 tcaattttca tcaaaatatt gccggctact atgccatcga tagaaaaacc gaaagaaaaa
    12841 acgaatcatt agacttgact tatcactggc aggattttaa cggaagatac agtaatacac
    12901 aaagaataaa tttttatgtg gatatttccg aatataccgg gcgaaacatt ccggctttca
    12961 aagaagacag ctatctgaaa actccaaaaa actatctttc ggctgtagaa tttgaactgg
    13021 cttacacaaa atttcctaat tccacgctta aatactatac caccagttgg gaaaatgtaa
    13081 ttgaacaata tttaaaatat aatgacttgg aaaattccat taaagttggt tttttggacg
    13141 atatagcaaa agaaatcaac aataaagcca aaaatccata ttcaaaaatg gaacttgcct
    13201 tttataacat tgccaatcat tataaatgga acgaaaaaaa cagcattttt acctcaaaag
    13261 gctttaagga tagctatacc aatgctacgg gaaattgcgc cgacatcaat ctttgccttg
    13321 taggactttt gcaaaaactc ggccttgagg cctatccggt aatactttct acacaaaaca
    13381 acggattcat caaacagact catccatctc tttccaattt taattatctg gtagcattgg
    13441 caaaaatcga tgggaatgac tacttgatgg atgcaaccga acctttgagc attatcaatc
    13501 aattacctgt cagatgcctt aacgacaaag gattaatagt caaaaaaggg caggcaaact
    13561 gggttgatat ggttaaagat gcaagctaca aaagtcagat tattcttacc tatgaacttg
    13621 atgaaaactt ggaagcaaaa ggaacatatt cttccatatt aagcaattat gcagcattgg
    13681 aaattcgcaa aaacattaaa tcgtacacat ccaatgacga gtattttaaa gaaatagcca
    13741 aaaataatcc cggcatcgaa acgaacgaat atcaaataga agggttggat tcgctgaata
    13801 tgcccgttca aatgaaattt gatttctcct acaacaatgc agtagaacag gccggtgatt
    13861 taatcatgct aaaacccttg ttgtttgaag ccatgaaaca atctccgttc aaattgtcgg
    13921 aaagaaccta tccggtcgaa ttcaattacc agaacaaaga caaaacaacc tgtcaaataa
    13981 aaattccaaa tggatatgca gtagaatccc tgccatcttc tatcaacctt accttgcctg
    14041 acaaatcggc acaattcctt ttctttacct cgataaaaga tgactacata ctgattgtat
    14101 cgaattatac cagaaacaag ttgctttatt tgccggaaga atatgaagtt ttaaaagaat
    14161 ttttcagaaa catggtagta aaacagaatg aaaaaattgt tttaaagaaa ttgtaatcat
    14221 gcgatttatt tttttagtct tagccttttt ttcgacattt tttgtcttct ctcaggaaaa
    14281 tctgaatgtg gcaaacatac ccgattcatt gaaagaaaat gccgatgcca tcatacgtta
    14341 tagtaacgta agttacgaaa taaaagaaaa taaattgata aaacggaaat cttttgccat
    14401 taccttgatg aacgataatg caaaagaaaa tgcttatttg gttatacctt acgatagcta
    14461 tacaaacatt aaaaagattg aaggttatat ttacaatgcc aatgggataa agaccgaaaa
    14521 attatccaaa gaaaacatta aagattatgc ggcttaccct tcgtttacac ttttcagcga
    14581 caacagaata aaagttgtgg gtttctccgg caaaaattat ccggttacca tagaatatat
    14641 ttacgaattg gaaagcaaag gcctcctcat gcccgaaagt tgggtgccga taaacgacta
    14701 ccggcgatcg cttgaaaagg cacaactgca aatcattgtt cctgatagca ttattcaata
    14761 taagtatcaa aatattcgtc cgacctttac aaaagaaacc gtaaataata acatcatcta
    14821 ttcttggaaa ataaatggaa taaaagcaaa ggaatcagaa cctttttctt tgccatttca
    14881 gttgatcatg ccaaaaatat ttgtaaatgc cactaaaaca aaatattttg aatatccagt
    14941 agaatttaac gattggaaat cctatggtaa aatggtattc aattttttga ccggcagaga
    15001 aatacttcta accaaaagtg tagaagaaat caattctctt tgtaaaaacg caaatactga
    15061 actggaaaaa gttaaaattg tttatgccta tgttcaaaat aaagttcgat atgtgaatgt
    15121 tactaagggt attggtggtt ttcagccttt accggctgcc gaagtagaca agtatggcta
    15181 tggagattgc aaggctcttt caaactatac cagagcttta ctgaatgctg tggggatcaa
    15241 gtcttactat gccgaaatcg gaaatggaaa caataaaaga atacagtttc ccgactttgc
    15301 aagcgtagac caaaccaatc atgtcattct ctttgttcca ttaaaatccg atagtttgtg
    15361 gttggagtgt accagcaata ccataccttt cggatatatt ggctcgggaa attgcaacag
    15421 acaagcattg gtcgtaacag aagaaggagg aaaattaatc gatacgcctt cctacaatga
    15481 attcgacaat ttgctttcca tttccggaga aatctttgta gaagaaacgg gaaacgcaca
    15541 tttgaaaatc aaaaaaacct attccaacga acttttcgac gaagtaatgc catttttaag
    15601 tctgtcgaaa caggaaacag aaaaatatat caataaatcg ataccaataa atggttttca
    15661 tctgacaggc tacagcttta tcaataaaag cagctataaa aacataaaag gagaacttgc
    15721 ttttgaagga gatatacttc aatatgcaga attaatgggt aaaagaatag ccttgaagcc
    15781 caatataatt agccgaagct attttggtct gaacaaaaaa agaaacaaca atgtttattt
    15841 ttcccagtcg tttactcaaa ccgataccat cgctattcac gttccctcgg gttataaatt
    15901 ggaatttgaa tatcccgaaa tttctttttc ttgccctgcc ggttattatt ccacaaaaat
    15961 aagtcaaaaa aaggcagaca gctttgtaat gataagaaaa ttaacaataa aaaaaggcat
    16021 ttatggaaaa gaaaattatc cggaaattta tgatttcttt aaaaactctg cgttagccga
    16081 caataaaagt tgtattttca ccaaattgta atccgcgaca caattttttt cttcagaatt
    16141 aaaatatccc attccgagca ttcatgtatt ttgaaaaaat cgagaatggg atttttcttt
    16201 ggctgtgtgt aaattgaaaa ttatcacgta aatttgtaat ttaaaatcaa aaaccgaaac
    16261 aatgatttca aaaattatag ctgaagacct tatcaataaa gtttactcgt cgcttcagga
    16321 tgcggaaaaa attgccatag ttgttcatgt taatcccgac ggagatgcaa tgggagcagc
    16381 gcttggactt tggcactatc tgatgacgat agaaaaagag ccaactgtta tttcacccac
    16441 tgcttacccc aacttccttt cctggatgcc gggagcaaac gaagtgctga tttatgaaaa
    16501 cgacaaggaa aaagccgata acgttcttgc ttcggcagaa ttaattttca cactcgattt
    16561 caacaacttg catcgactcg aagaaatggc cgatgcagtt gcaagttcgc cggcccctaa
    16621 aattttggtt gaccatcatc tccatcccga atcttttgca aaaattacca tttcctatcc
    16681 ggaaatttct tctaccagcg aaatgatttt tcgcctgatt tgccgaatgg gcgactttgc
    16741 aaatattaat cttgcctgcg ccgaatgtat ttatacaggc atgatgaccg atacgggaag
    16801 ttttacctac aattccaacg atccggaaat ttatatcatt atttccgaac tgattaagat
    16861 aggaatcgat aaagatgaaa tttaccgaaa agttttcaat acttattctg aaaaccgatt
    16921 gcgattgatg ggattttgcc tttacgagaa aatgcaaact tttcctgaat ttcgtacggc
    16981 catcatttcc ctcacgcagg aagagttatc acaattcaac tatgtcaacg gagatgcaga
    17041 aggatttgtt aacatccctc tttcagttaa aggtattgat ttttcagtgt ttttgcgtga
    17101 agaaaaaaac agaattaaaa tttcgttgcg ttcgcaagga aaatttcctg ccaataaagt
    17161 agcagccgac ctgtttggcg gaggcggcca tctgaatgct gccggagccg aaagttatgc
    17221 ctccatgcaa gaaaccattc atactttgtt gaatgcattg cctgattatc aggtattttt
    17281 tgaagaataa aaaaattatt ttcactattt gaaaaacaat cataacttct tgaagtaata
    17341 aatcaaaatg tttttcaaaa aaaatttagt ttgaacaaat aaaaaaccct atgaaaccca
    17401 catgtattgc atacacaaac acgaatgaat ataatattca gaca
//
LOCUS       DAOSIB010000043        17187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_3526_length_17187_cov_9.191805, whole genome shotgun sequence.
ACCESSION   DAOSIB010000043 DAOSIB010000000
VERSION     DAOSIB010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17187
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3526_length_17187_cov_9.191805"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..1720)
                     /locus_tag="PLD44_05290"
     CDS             complement(179..1720)
                     /locus_tag="PLD44_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98781.1"
                     /translation="MKKGIGIVALLLNSFMISAQSELDISKFVIPDIVGTARYMSMGG
                     AMGAVGGDASAIKDNPAGLGIYRSSEMTGTLNILRQNTDANWYGVNSANNLYKLGTNN
                     FSLVISSATQRSKSGKTSGLQNSNFSFSFQKLKDFNRQLNIHGGSSSSSMTDYMAYFT
                     QGIPETDLQSTDSYEPFDNTYIPWLSVLAYEGYLINPGTSNSWTSSLLDGERVTPYYY
                     LTENGSINEYSFSWAGNFSNQFYLGVTANIQSIYYNATTNYNEAFGQGGSMNLNNTIK
                     SSGNGFNLNIGAILRPIDAFRMGISFQTPTVFSISEDYTSLLNFDTEQRGYVRTPNGY
                     NSYKFQAPLQFTVSVAYLFGKNGLISAEYDYIDVKTSKLMDNNNSFYNFMEENEGMNM
                     AYNSASTIKLGAEYRLTDNFSLRGGYAFMDKVNPDNAQKFMKDNTIRTDTEYFLHNST
                     QYITGGFGYREESWFLDFAYMNKLNKEKFHPYNSNNLAEFVQGSPANVNTTNNNFVIT
                     LGFKF"
     gene            complement(1814..3148)
                     /locus_tag="PLD44_05295"
     CDS             complement(1814..3148)
                     /locus_tag="PLD44_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98782.1"
                     /translation="MKPAKLCILFLSVLLALPGYGQKNKASIDDVYFSWDDAKNTAKM
                     STESNKKPNTKNGAKEIIFIDRNEDAQNNSSRLLIAKANDSTTVDSDTLAADEGYYLR
                     DFNGSESDYEYAERIRRFHNPKFTIHISDPEYMDIYFLDDSQWNVYVGGSYAWITPTW
                     TNPAWFDYYYRPFSYNSWYWRSSLYYPYSFYGYHSWVYSPWYYDPWYYDPWFYYPAYY
                     RPYYGYPYYGYYGWSRPFYKSVDPSHNEAYRRQVANGLRNSNTNSSTRAGILSGVAGG
                     SSSTGRSGNPYTVVSGSGSRSSVSSDINSTGRRSSTSSYDNSRSSINSGRTTTGTRSQ
                     SEVTGTTGTRSTETYSGNSSRRTTETINTRPRTSSGNSGNSVATPQTGSRSSYTPSSG
                     SSERRSSYTPSSSDNSSRSSGSSYSGSSRSSGSSSGGGSSSSGSSRSSGGRR"
     gene            3379..4218
                     /gene="prmA"
                     /locus_tag="PLD44_05300"
     CDS             3379..4218
                     /gene="prmA"
                     /locus_tag="PLD44_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOF98783.1"
                     /translation="MDYLEVNFTLSPYEEFIADLLMETLGEIGFESFQLADQQFFAYI
                     QESNFNQKILDDKLSKFEYPVSIDYNILLLENKDWNEEWEKNFFQPILIDNDCVVHSS
                     FHQKVPEAKYDIVINPKMAFGTGHHETTALMLGELLKMNLIKKNLLDMGCGTAVLSIL
                     ASLRGASSVVAVDIDDWCIENSMENIRLNKIKNIELRKGGAEVLKDVHVDIVLANINR
                     NILLKDMKFYAACLPTGGELYLSGFYVEDIPMIEAEAEKNGLQLAYFKEKNNWALVKT
                     IKK"
     gene            complement(4831..7851)
                     /locus_tag="PLD44_05305"
     CDS             complement(4831..7851)
                     /locus_tag="PLD44_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOF98784.1"
                     /translation="MAGKKYQFSKSFPENPISKAWDNEELIECLAYGEGKWLLVTDPD
                     SSYSGQRWSTRSEFPKDAISTGWNDGLDISWLGYGEGVWYVVMSGSTGVEEQRWITDG
                     NFPADEIKEAVKSGESIIRLVYGNDRWAAVFGKMSKYGTQIFGLYSDFPAKTINDYWA
                     KGYFITSLSPANGKWGLVMTKDAPFENQFYVSHKQFPEEDVKSKLNEGYEITNVSYGN
                     GIWLVVMSIPFEEPADNKEEEFTFDPDESEVEQKAQPAKELNISPKAMRYYQTGMEYF
                     DRKEYQKAIDYFEDALKLSPEFPSALNSIGAAWSWLGDMEKALKYYQESFRLDASNPV
                     VFSNLVYSLNDADKKDELFKIAMECSSSVLKEVDDATPLYQAGRVLLEKGQNEKAKVY
                     FKLALKLEPDEKLFKEALEEAEAEPKKTPEPSSTPSKSEPAQSLDELLSEVNKLVGLN
                     NIKTDINNLMKYIRIEKMRKERGLNSNPISLHSVFQGPPGTGKTTVARLLGKIYQSLG
                     LLKSGHVVEVDRSGLVAEYIGQTALKTNKVIDSALDGILFIDEAYALIPEGGKGNDFG
                     QEAVDTILKRMEDSRDRLIVIVAGYPAEMNRFILSNPGLQHRFNRYFTFNDYQPEELL
                     EIFRRIAESANFKIAPDAMEKVSKYLNYVYQSRTDTFGNARLVRNLFEEVVQLQSARL
                     AEMEHISDEDLCTITLEDIDKAVSDEYKEEITESLDEIMAELNGLIGLDNIKNDVEQL
                     LNYIRVEKMRSEQGLTTQSLSLHAVFQGPPGTGKTTVARLMGRIYKSLGLLPKGHVVE
                     VSRNDLVGEYIGQTGPKTDQVIDSAMHGILFVDEAYSLNPGGQGNDFGREAIETLLKR
                     MEDDRDKFVVILAGYPDEMKDLIETNPGLKSRFNRYFNFVDYKPDELMDIFEGFLKKR
                     SMQLEPAAEKRLIGHFIDAWESRDKHFGNGRFARNLFEKIMQAQSDRVAKMNEISPEV
                     LCLITAEDVESVISTVQPDQPKQRPTIGFKPPEG"
     gene            complement(8039..8401)
                     /locus_tag="PLD44_05310"
     CDS             complement(8039..8401)
                     /locus_tag="PLD44_05310"
                     /inference="COORDINATES: protein motif:HMM:NF039385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6232 family protein"
                     /protein_id="HOF98785.1"
                     /translation="MAEEKIYFEEGDVKVTNARFLTYGKMHSMANVTSVAKYVVKPKR
                     TGPIVLGIIGLICFAFKWWLAVLLLAGAVAWWMTQKNNYIVSLASSSGNQEALSSTDE
                     AFIDRVVNALNDAIAERG"
     gene            complement(8720..8845)
                     /locus_tag="PLD44_05315"
     CDS             complement(8720..8845)
                     /locus_tag="PLD44_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98786.1"
                     /translation="MLVPDFKFTIHVDSDNYINKEEMNDALVAFLVYVFTEGMVK"
     gene            9328..11640
                     /locus_tag="PLD44_05320"
     CDS             9328..11640
                     /locus_tag="PLD44_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98787.1"
                     /translation="MILNEKYYNALINKYSIRRDCDKMVCLSTSMILDNYRSRNVYFN
                     FPKDYNLQKITNLLYQDLSKQIFLNHADLTDYSVGDRLKRIKEKGKNIYVITKIEWPT
                     YTLHKENDENHTVIQGITYDNLKLNYIKIKHNINHNTFLKYSNFFKDINEYGFLPTHF
                     TKKLVLIAGQTIWNGLENRSSIPSIYLPNTREGEQTIRKSIEGLEDCIAYITPKYEVC
                     YEEIFKKNIAIDTILVCDTDLDSVPQIIQDQSKYHFKLIVLSNESNKQKPNNITLWNW
                     QKEEIQLLEEKNSNKIEIACIEDEEFDCYMQHFEECMKYVSELEYPLKLKSYGYYLRL
                     ALNAIQEEQFDYLLMRLKSDKELEHNEGGYEDFGENNPKKALKNLISYLKVNNPKFKK
                     LNEVISKTTKKTLYIVAREDVEYYRTNRNKNCQFITQKELKTFIKKNNTHKKTIIFNS
                     FNGSKDFDFIYNLPNDVRLVLYRQEKDLYFRQLQNHKKQLEEELTSTDRFSICGIKYE
                     PIVKDEVKISPTLEQIIERLDQRSNTAYDGYKSESDSILDDLEEEDITYKIFFTNGFE
                     TEIKSNETVFDEKGDLIKAYKLKPNYKIRVYPKEQFAENLLQIAIEVEPEKFGKINEH
                     SIYWQELLKLLDNTFSSRDLLYKKLKERGLKVLPATVDTYFGGKIKFPMYNSDLQAIC
                     AVAEEIMPESKILDEMFLQLKKSKQLYNSTMIALGRGVKQELRQFLKEKTVGEILQKK
                     NFTEDTLRQFINKFMPLLTISKIEKIDNEQ"
     gene            11630..15280
                     /locus_tag="PLD44_05325"
     CDS             11630..15280
                     /locus_tag="PLD44_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOF98788.1"
                     /translation="MNNREKIITRVQKELIGPGSDLFECSDKVNFSDEIIADKPLLRY
                     FSGILYPKKNAIDDFPEVDDESDETPIEIAEEKNDNATASDNNDIIQPVISKEKEDDK
                     EVQYSASTFFPSQYGISFAISKDCPELKIKISFGNYKKAKAKEIAIEYEGDSVELIEQ
                     YELDKFVEFDAENKLLKQTKELNKKEKQQRSSCLNSLKKDHRDSGLYKTLTKLFFKDK
                     YKRYENKIETTISIQDIKKSDNQHYRILLSNIDGVNTEDWWKGVINKEKTFITDHLWL
                     HVKLYDQPNNIYVVKIFLENTLLFPHTKFAFSKESLNMACLFQTEIKVETPYLQPFND
                     YKNNLYKSDEDRMLDFLYREKLSYGIGHNTACTWENCDDDTQKPRWVSSTFLPQFNVK
                     NQSTNISSVDKKCLEIKELSEFNNNKKTIISNLEKLAQSYSKWIDEEEKIKLLNEHDR
                     LLGLNNIKKCRTIQARMQRGIELLETNSMAFHAFQLANTAIYLQMFQNQWHFSKKKDG
                     FEPFEIIENPQLSYQEYATKDYPDPNNTPRWRPFQLAFILQCLPSFIDENSNDRNLVD
                     LLYFPTGGGKTEAYLALSAFLIFWRRLNYPDSYGGVNIIIRYTLRLLSAQQFERATKL
                     ILACEFIRKNHTDLGNERISIGFWVGGSTIPNKINNDQNCAEQELQKVQDRLNKNQPS
                     INPFQLSNCQWCNTKIIGKTQPNVNPSIGHRIYKKSLKSYCLNKNCFFSENNEGLPIV
                     LVDEDIYKNPPTVLFATVDKFAQLAWREEATSLFNYNGNRKPELIIQDELHLLNGPLG
                     SLVGLFENVILSLCTTDNQKPKIIASTATIKNVEEQVKGLYNRPVQVFPQYATNADDT
                     FFSKTILESKRRYVGVLPTGRTQTMTSLRLNAALLFARLEIWKDSVDKTEADQFWTIV
                     SYFKSLKHLGRFSNKIIAELIPEIKQLQVRYLMNVFPFNNNYSKLPYRNLELTSRIPN
                     ERIKKNLDKLNISFDGNLEKYQSFDIVLATNMISVGLDVERLNIMLMNGMPPNTAEYI
                     QASSRVARKNEGVVFSLFDPNNTRDLSYFEGFIPFHKTFYKQVEPISVTPFAENALDK
                     MLFTSMIAYFRHKMGYSANNMATALNENGNKQKLMSEFNQLFNNHSFADEEDKQVIST
                     IINELIQQWDNKIQNSPKDSLFYYGGKHETERKKNLLKSVQEKEKTDDKLIGMQSMRS
                     VEPSVTIKIKKF"
     gene            15284..17164
                     /locus_tag="PLD44_05330"
     CDS             15284..17164
                     /locus_tag="PLD44_05330"
                     /inference="COORDINATES: protein motif:HMM:NF038324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOF98789.1"
                     /translation="MAEKINHGRSKMVSSYGGVGSVIDTPDASIIIETFDNWGYHNYD
                     DQEKHKHIILDDRLVFRLRTRFPNLKYLMSIPIEKDGKVQPQANYFPKWFYCPHCDRF
                     MHINDWKKHWDSGKGEFDLQCFDPKCKGQHLEQVRFVMTCENGHIQDLPWEYWNNREQ
                     IVENIELPLNETENIEDDDIEDNNEQESQSKIRLIYKKCCPEQELYYSVSNENTDLSG
                     IHIKCKNCGKSETLKGIFGFSQNCGGRKYWLGLKNGQYQGEPCNKRAKVKIKTSNSIY
                     YANTLSSLWIPEKQILDLSAELRTEIDGIVNDQDFEQKDLEKFARRKGIAIEIINQYL
                     DNTDTAYIPEIVYRQAEYNYFLNKQQPEDQVIKFSQINVSGKLNGFEKLVKINRLKKI
                     TVQTSFTRNEPIDADSILIDPESGNYQYMVKPQSVSKNSFETHLLPAIENYGEGILFV
                     LDEQKLKEWGKNDIITERVKNIQTHAEDSDWQFHKIEAENLSARKILIHTLSHLLIRE
                     FEYVCGYPMSSLQERLYVSENMNGFLISAYDGTDGYLGGLAKLCNNLENLQAIITNAI
                     QRAKECSLDPICYESEGQGVAMLNLAACHSCTLIPDTSCELSNLFLDRRLLIDDEFGY
                     FI"
BASE COUNT         5928 a         3026 c         2935 g         5298 t
ORIGIN      
        1 ttcttgtaaa ataaaagtat tatgttagag aagttatttc caaatttcaa caacttcgtc
       61 ttttttattt ggttccaact ttttctggga ctgttgtaat aaaatgattt taataaaaaa
      121 accggctgac ttacaaccgg ctttctgcaa aatttaaaac tcaggacttg atagaaattt
      181 aaaatttaaa acctaatgtt atgacaaaat tattgtttgt tgtgttgaca tttgccggtg
      241 aaccttgaac aaattctgcc aaattatttg aattgtaagg atgaaatttc tctttgttta
      301 acttattcat ataggcaaaa tccaaaaacc atgattcttc acgataacca aaacctccgg
      361 tgatatattg ggtactgttg tgcagaaaat attccgtatc ggtacgaatg gtattgtcct
      421 tcatgaattt ttgtgcatta tcgggattta ccttgtccat aaaagcataa ccgcctcgta
      481 atgaaaaatt atcggttagc ctgtactcgg cacccaattt aattgtgctg gccgaattgt
      541 atgccatatt cataccttcg ttttcttcca taaaattata aaaagaatta ttattgtcca
      601 ttaacttgct cgttttcacg tcgatataat catattcagc actaattaat ccattttttc
      661 caaaaagata agcaacactg acggtaaact gaagtggcgc ctgaaattta taagaattgt
      721 atccgtttgg ggtgcgaaca tatcctcttt gttctgtgtc aaaattaagt aacgaagtat
      781 aatcttccga tatagaaaaa actgttggcg tctgaaaaga aatacccatt ctgaaagcat
      841 caataggacg caaaattgca ccaatattca ggttgaaacc attacccgaa gatttaatgg
      901 tgttattcaa attcatactt ccaccttgac caaaggcctc attataatta gttgttgcat
      961 tgtaataaat actttgaatg ttggctgtta ctcccaaata aaattgattg ctgaaatttc
     1021 ctgcccaact gaaagaatac tcattaatag agccattttc ggttaaataa tagtaaggag
     1081 taactctttc gccatccaac aacgaagatg tccaactgtt actggtacca ggattgatta
     1141 aataaccttc gtatgctaaa actgacagcc atggaatgta tgtattgtcg aacggctcat
     1201 aagaatctgt actttgcaag tcggtttcag gaattccttg agtaaaataa gccatgtaat
     1261 cggtcatgga agacgatgat gaaccaccat gaatatttaa ttgacgattg aaatctttca
     1321 gtttctgaaa tgaaaaggaa aaattagaat tttgcaatcc gctggttttt ccacttttac
     1381 ttctttgggt agcagaagaa ataaccagcg aaaaattatt ggttcccaat ttgtaaagat
     1441 tattcgcact attcacacca taccagtttg catccgtatt ttgccttagg atatttaaag
     1501 ttcccgtcat ttctgaactt cgataaattc caagacctgc cggattatcc ttgatagcgg
     1561 aagcgtctcc gccaacagct cccatagctc ctcccatgct catatagcgt gctgttccta
     1621 caatatcggg aataacaaat ttcgaaatat ccaattcact ttgtgcactt atcatgaagc
     1681 tattcagcaa taaagccact atcccaattc cttttttcat atagattatt tttttaaaaa
     1741 gtagttaaag aatactcttt ctctttccct ctccgtttct caaaatttta tagatttaag
     1801 taaattctga tttttatctc cttcctccgc tgcttcggct cgatccacta cttgaacttc
     1861 ctccgccgga actgctgccc gaactgcgcg agctaccgga ataggaactg ccggaacttc
     1921 gcgaactatt gtcggaactg ctgggcgtgt aagaagatct tctttcagaa gaaccggaag
     1981 atggagtgta agaacttcgt gaaccggttt gaggcgttgc aaccgaattt ccgctatttc
     2041 cggagctggt tctcggtcgg gtattaattg tttctgtggt tctgcgcgaa gaatttccac
     2101 tataagtttc cgtcgaacga gttcctgttg taccggtaac ttcactctga cttctggttc
     2161 cggttgtggt tctgcccgaa ttgatagaag atcttgaatt atcataactt gaagttgaac
     2221 ttctgcggcc agtcgaattt atgtcggacg aaacagaact tctggaaccg ctaccactta
     2281 caaccgtata aggattacca cttcggccag tcgaagagct tccgccggct acgccggaga
     2341 gaatgcctgc tcttgtagaa ctattggtat tcgaatttct caaaccatta gcaacctgac
     2401 ggcgataggc ttcattatga cttggatcaa cagatttgta aaaaggtcga ctccagccgt
     2461 aatatccata ataaggataa ccgtaataag gcctgtaata agcgggataa taaaaccatg
     2521 gatcgtaata ccatggatca taataccacg gtgaatagac ccacgaatgg tatccgtaaa
     2581 aagaatacgg ataataaaga ctgcttcgcc aataccagct attataactg aacggacgat
     2641 agtaataatc aaaccatgca ggattggtcc atgtaggagt aatccatgcg taagacccgc
     2701 ctacataaac attccattga ctatcgtcca aaaaataaat atccatgtat tcggggtcag
     2761 aaatatgaat ggtaaatttc gggttgtgaa aacggcgaat tctttcagca tattcgtaat
     2821 ccgattcgct tccattaaaa tctctcaaat aataaccttc atctgcagca agtgtatcag
     2881 agtcaactgt tgtactgtcg ttggctttag caatcaataa tcgtgaagaa ttattctgtg
     2941 catcttcatt tcggtcgata aaaataattt cttttgctcc atttttggta ttgggctttt
     3001 tattggactc agtactcatc ttggcggtat tttttgcatc atcccacgaa aaatacacat
     3061 cgtcgatcga tgctttgttt ttctgtccat atcccggcaa ggcaaggaga acagataaaa
     3121 ataaaataca taattttgcg ggtttcatga tattacctcc tgtgttttgt agatagattt
     3181 tgcaattata taagcatcaa taattgtacc attgtaagaa aaaagcatac ttatttttac
     3241 ggttttacta aaaagttagt caattgaaga actcaacaaa aatatgtaat tttgttgagt
     3301 tttatatttt agatttactt tttttgaaaa ggtttaattc gtttatggtt tcttagcgat
     3361 tttaacgcgt caaatattat ggattatctt gaagtgaatt tcacattgtc tccatatgaa
     3421 gagtttattg ccgatctttt gatggagaca ttgggtgaaa taggatttga aagttttcaa
     3481 cttgctgacc aacagttttt tgcttacatt caagaaagca atttcaacca gaaaatactc
     3541 gatgataagt tgtccaaatt tgaatatcct gtttccattg attacaatat attattgttg
     3601 gaaaataagg actggaacga agaatgggaa aaaaattttt ttcagccgat attgattgat
     3661 aacgattgtg tagttcacag ttcttttcac cagaaggttc ccgaagcaaa atacgatatc
     3721 gttatcaatc caaaaatggc ttttggtacg ggtcatcatg aaactactgc attgatgctg
     3781 ggtgagttgt tgaaaatgaa tctgattaag aaaaatcttt tagacatggg ttgcggaaca
     3841 gccgtattgt ccattctggc atccttgcgc ggggcttcat cagtggtagc ggttgacatt
     3901 gacgactggt gcattgaaaa ttcaatggaa aacattcgat tgaataaaat aaaaaatatt
     3961 gaactcagaa aaggtggagc cgaagtgctt aaggatgtac atgttgatat agtgcttgcc
     4021 aacataaaca gaaatatttt gctgaaagat atgaaatttt acgcagcttg tttgccgaca
     4081 ggaggagaac tctatctaag tggattttat gtggaagata ttcctatgat tgaggcagaa
     4141 gccgaaaaaa atggattgca gttggcgtat ttcaaagaga aaaacaactg ggcattggta
     4201 aaaacaataa aaaaataaac atctatttac acataaaaat atttattaat attgaaagct
     4261 tgagaatata aattcctgac ttcaacaatg cttcacccta aaaattttcc acaacttatc
     4321 atcaaaaatg ggcaacgatt aatttgtttt ttagagtaat ttacatatct cgatcaatgt
     4381 tttatcactt ttgggcagtc tgattctcgg agcaataatt gttttaacta tattcaaacc
     4441 tttatatctt cgctctaaaa tgaatttttg cctttcattt ctcggctatc aagccttatc
     4501 tttcttaact ttcatcagaa tataatttat tttttaaagg tctgatggaa cccacatgtc
     4561 tgtatttgtg tttgtgtatg caatacaggt gggtttctaa aaaatttcct ttttctgcca
     4621 ccgctacact catcgtacct gaccgattat ttacttcctg acatatataa atgatatcat
     4681 tattttagca gcatccaaat aggtgatgca aaaaactgca aatatctcca cacatagttg
     4741 tttccgaatt aacctttaaa tatgtcgagt cagaaatgag tgtcaaagtc aacagggtaa
     4801 agaagatgaa ttcaattcac aaatctctta ttatccttcg ggtggcttaa atccgatggt
     4861 tggacgttgt ttgggttgat cgggttgaac ggttgaaatg acactttcaa catcttccgc
     4921 agtgataaga caaagaactt ccggcgaaat ctcattcatt tttgcaaccc ggtcgctttg
     4981 tgcctgcatt attttttcaa ataaattccg tgcaaaacgt ccatttccaa aatgtttgtc
     5041 gcgactctcc caagcatcaa taaaatgtcc gataagacgt ttttcggctg ccggttccaa
     5101 ttgcatggaa cgtttcttaa gaaagccttc aaaaatatcc atcaattcat caggcttata
     5161 atctacaaaa ttgaaatagc gattgaaacg cgatttcagt cccggattgg tctcaatcaa
     5221 atctttcatt tcgtcgggat aaccagccaa aataacgaca aatttgtcgc gatcgtcttc
     5281 cattcttttc agtaaagttt caattgcttc gcgaccaaaa tcatttccct gcccgccagg
     5341 attaagagaa taagcttcgt ctacaaaaag aataccgtgc atagccgagt caatcacctg
     5401 atcggttttt ggccccgttt gtcctatata ttctcctacc aaatcgttcc gtgaaacttc
     5461 taccacatga cctttgggca acagtcccaa agatttgtaa attcggccca ttaatcgggc
     5521 aactgttgtt tttcccgttc ccggaggtcc ttgaaataca gcatgtaacg acaggctttg
     5581 agttgtcaag ccttgttccg aacgcatttt ttctactctg atataattga gcaattgttc
     5641 gacatcattt tttatgttat ccagtccgat caaaccattc aactcagcca ttatttcatc
     5701 caaactttcc gtaatttcct ccttatactc atcggatacc gccttatcaa tatcttccag
     5761 cgtaatagtg caaagatctt cgtcggaaat atgttccatt tcggccaatc gagctgactg
     5821 caactgtaca acttcttcaa aaagatttct caccagacgt gcgtttccaa aagtatcggt
     5881 acgcgattga taaacataat tcagatattt agaaactttt tccatcgcat cgggcgcaat
     5941 tttaaagttg gccgattccg caattctcct gaaaatttcc agcaattcct ccggctgata
     6001 atcattaaaa gtaaaatatc tgttaaaacg atgttgaagt cccggattgg acaagataaa
     6061 tcgattcatt tcggccggat aaccggcaac aattactatg agtcggtcgc gcgaatcttc
     6121 cattcttttc agtatcgtat cgacggcttc ctgcccgaaa tcattccctt ttcctccttc
     6181 cggaatcaag gcatatgctt catcgataaa aagaatgccg tccagtgcgc tgtcaatcac
     6241 tttattggtc tttaaagctg tctgtccgat atattcggcc accagacccg aacgatccac
     6301 ttctaccaca tgtccgcttt tcaataatcc caacgattga taaattttcc cgagaagtcg
     6361 tgccactgtg gttttccccg tgcccggagg gccttgaaat accgaatgca aagatatggg
     6421 attggaattc aaccctctct ccttacgcat tttttctatt ctgatgtact tcatcagatt
     6481 gttaatatcc gttttgatat tgttcaaacc aaccagttta tttacttcgg acagcaattc
     6541 gtccagcgat tgagcaggtt ccgacttgga aggcgtagag gacggttctg gcgttttctt
     6601 gggctctgct tcggcttctt cgagcgcttc cttaaagagt ttctcatccg gttccagttt
     6661 taaagccagt ttaaaataaa cttttgcctt ttcgttttgc cctttctcga gtaaaactct
     6721 tcctgcctga tacaaaggag tagcatcatc cacttctttc aataccgaag aagaacattc
     6781 cattgctatt ttgaataatt catccttttt atccgcgtca tttaatgaat acaccaaatt
     6841 ggaaaataca accggattgg aagcatcgag tcgaaaggac tcctgataat atttcagcgc
     6901 cttttccata tcaccgagcc acgaccatgc cgcgccaata ctgttcaagg ctgatggaaa
     6961 ctccggcgaa agttttaagg catcttcaaa ataatcaatg gctttctgat attcttttct
     7021 gtcaaaatat tccatgcccg tctgataata tctcatggct ttgggcgaaa tattcaactc
     7081 cttagccggc tgggctttct gctcgacttc cgattcatcc ggatcaaaag taaattcttc
     7141 ttccttgttg tcggccggtt cttcaaaagg tatagacatc actaccagcc atataccgtt
     7201 gccataactg acgttggtaa tttcgtaacc ctcattgagc tttgatttga catcttcttc
     7261 cggaaactgc ttatgtgaaa cgtaaaactg attttcaaaa ggagcatcct tcgtcatcac
     7321 caaaccccat ttcccgttgg cagggctgag tgaagtaata aaatatcctt ttgcccagta
     7381 atcgtttatg gttttggcag ggaaatcgct gtagagtcca aaaatttgag tcccatattt
     7441 cgacattttt ccgaacactg ctgcccatct gtcattgcca tacacaagcc ttataatcga
     7501 ttcacccgat ttaacggctt cttttatttc gtcggcagga aaatttccat cggtaatcca
     7561 tcgctgctct tctacacccg tactgcccga catgacaaca taccaaactc cttctccata
     7621 accaagccaa ctgatatcca agccatcatt ccatccggtg gaaatggcat ctttcggaaa
     7681 ctcggaacgg gtactccatc gctgacccga ataggaacta tcgggatcag ttaccaatag
     7741 ccatttgcct tctccataag ccaaacattc tattagctct tcattgtccc aagcttttga
     7801 aatgggattt tcaggaaaac tttttgaaaa ttgatatttt ttccctgcca taatgtttaa
     7861 tacaattaaa tgttaaaatt tactaattcc gaaattcaga tgcccgaaaa aaattactat
     7921 ttgcctgatt atcaaataaa tatacaaata cttatatgtc aatgaaggtt cctttttttc
     7981 acgaaaaaac ctgccggcaa aagtcaatac cggcaggttc aaattctttt gaaaaagctc
     8041 aacctctttc tgcaatagca tcgttcaaag cattgacaac tcggtcaata aatgcttcat
     8101 cggtactgga aagcgcttcc tgatttcccg atgagcttgc caacgatact atatagttgt
     8161 tcttctgtgt catccaccag gctacagcac cggcaagcaa cagaacagcc aaccaccatt
     8221 taaaagcaaa acaaattaaa ccaataatac ctaaaacaat cggccctgta cgttttggct
     8281 ttactacata cttggcaaca gaggtaacat ttgccataga atgcatttta ccataagtta
     8341 aaaagcgggc attggtaact tttacgtcac cttcctcaaa ataaatcttt tcttctgcca
     8401 tacttgtgat tttttaatga taaatattgg gtgaattttt tttaattcag taaaatgaac
     8461 taaaactttg taaaaatata atattgttta atattataga ttaaggcagt tgtatattta
     8521 agaatagttt aaataaatta tacataatta tgaattgtat cctgtcaagt ttcacttatg
     8581 aaaaaacttg gattttcaat aggcttcaaa agcataattt aatttttaaa ttcagaagga
     8641 ttttgtttga aaatttcagg tttcatttca aaccattttt caactgcttg aaaaggagtt
     8701 tttacgcttt gttcttttct cacttcacca tgccttccgt aaaaacatag acaagaaaag
     8761 ccaccagcgc atcattcatt tcttctttat ttatgtaatt gtccgaatcc acatgaattg
     8821 taaatttgaa atccggcacc aacatttcac caatatgctt atatcaactg acaactgtta
     8881 gctgaaaact gataactgtt tgtggttgct ttttcttttt gtcataagtt gtcaaaatca
     8941 gatattttct ttgaagcgta aaacttttct tttgcgtctg ttttatgtgt gaataattgt
     9001 ttatctttgc catatactga aaatattcat aaataaaagc aatacaaagc ataacaacgg
     9061 agctgtttta gtaataaaaa tcaaaaaaga aaggctatga aatgcaatac gttgttaaaa
     9121 tgaataggaa aatcgaatgg cattgtctca gcatataatt gctctttcca gtaattctct
     9181 ttattttcta atgacggaag gttattattt aacgtaggtt gcagataaag ttactgaacg
     9241 atttgataat gagatagact tggcagaaaa ttgttagaga ctttcactaa aacagataaa
     9301 aaataattca aaaactatct gaaagagatg attcttaatg aaaagtatta taatgcccta
     9361 attaacaaat attcaatccg gcgagattgt gacaaaatgg tttgtctttc tacttctatg
     9421 attttagata attatcgaag caggaatgtt tattttaatt ttcccaaaga ttataattta
     9481 caaaaaatta ctaatctgtt ataccaagac ctttcaaaac aaattttctt aaaccacgca
     9541 gatttaactg attattcagt tggcgataga ttgaaaagaa ttaaagaaaa agggaaaaac
     9601 atttatgtaa ttacaaaaat tgaatggccg acctatactt tacataaaga aaatgacgaa
     9661 aatcatactg taatacaggg tattacatat gacaatttaa agctaaatta tatcaagata
     9721 aagcataata taaatcataa tacttttctt aaatatagta atttctttaa agatattaat
     9781 gaatatggct ttttacctac tcatttcact aaaaaacttg ttctaattgc aggacagaca
     9841 atttggaatg gtttggaaaa taggagtagc ataccttcta tttatctgcc aaatacacga
     9901 gaaggtgaac aaacaataag aaaatctatt gaaggattag aagattgcat tgcatacata
     9961 actccaaaat atgaggtgtg ttacgaagaa atttttaaaa aaaatattgc tattgatacc
    10021 attttggttt gtgatacaga tttagattct gttcctcaaa taatacaaga ccaatcaaag
    10081 tatcatttta aattaattgt gctttcaaat gaaagtaata aacaaaagcc aaataatatt
    10141 actctttgga attggcaaaa agaagagata caattattgg aagaaaaaaa tagtaataaa
    10201 atagagatag cctgcattga agatgaagaa tttgactgtt atatgcaaca ttttgaagag
    10261 tgtatgaagt atgtttccga acttgaatat cccttaaaac tcaaaagtta tggatattat
    10321 ttacgattgg cactgaacgc tattcaagaa gaacaatttg actatttgct tatgcgtttg
    10381 aaaagtgaca aagaattgga acacaacgaa ggaggctacg aagattttgg cgaaaataat
    10441 ccgaaaaaag cattgaaaaa tctgatttct tacttaaaag taaacaatcc aaaatttaaa
    10501 aagctcaatg aagtaatatc taaaacgaca aagaaaaccc tatatatagt cgccagagaa
    10561 gatgttgagt attatagaac aaatagaaac aaaaattgcc aattcattac ccaaaaagaa
    10621 ctcaaaacat tcattaagaa gaacaacaca cataaaaaaa ccattatatt caactcgttc
    10681 aacggttcaa aagattttga tttcatttat aatttgccaa acgatgtaag attagtgttg
    10741 tacagacaag aaaaggattt gtattttagg caattgcaaa accataaaaa acaactcgaa
    10801 gaagaattga caagtactga cagattttca atatgtggaa taaaatacga gccaatcgtg
    10861 aaagacgaag taaaaatcag tccgactttg gaacaaatta ttgagcgact tgatcaacga
    10921 tcaaacacgg cctatgatgg ctataaaagt gaaagcgata gtatattaga tgatttggaa
    10981 gaagaagata ttacatacaa aatatttttt acaaatggat ttgagactga gataaaaagt
    11041 aatgaaaccg ttttcgatga gaaaggtgat ttaatcaaag cctataaatt gaagcccaat
    11101 tataaaattc gcgtttatcc gaaagaacaa tttgcagaga atctattaca aattgctatt
    11161 gaggtcgaac ctgaaaaatt tggcaaaatc aatgaacatt caatttattg gcaagaatta
    11221 ttaaaacttt tggataatac tttctcaagc agagatttac tatacaaaaa attaaaagaa
    11281 cgtgggctga aagttttacc tgccacagtt gacacttatt tcggtggaaa aataaaattt
    11341 cctatgtaca attcagactt acaagctatt tgtgctgttg cagaagaaat aatgcctgaa
    11401 agtaaaatct tggatgaaat gtttctacaa cttaagaaat caaaacaatt atataacagt
    11461 acaatgatag ctttgggacg aggcgtaaaa caagaattac gacaattttt aaaagaaaaa
    11521 acagtgggcg aaatcttaca gaaaaagaat tttactgaag atactttacg gcaatttata
    11581 aataagttta tgccattatt aacaatttca aaaatagaaa aaatagacaa tgaacaatag
    11641 agaaaaaata attacaagag ttcaaaaaga attaatcggt cctggctctg atttatttga
    11701 gtgttcagat aaagtaaatt tttcagacga aattattgct gataaacccc ttttacgcta
    11761 tttttcaggt attttatatc caaagaaaaa cgctattgac gatttccctg aagttgatga
    11821 tgaaagcgat gaaacgccga tagaaattgc cgaagaaaaa aatgacaatg ccactgccag
    11881 tgataataat gatataatac aaccggtaat aagcaaagaa aaagaagatg ataaggaggt
    11941 tcaatattct gcaagcacat ttttcccttc acaatatggc atttcattcg ctatttcaaa
    12001 agattgtcct gaattaaaaa taaaaatttc atttggaaat tataaaaaag ccaaagccaa
    12061 agaaattgct attgaatatg aaggtgacag cgtcgaatta attgagcaat atgaattaga
    12121 caaatttgtt gaatttgacg ctgaaaataa attacttaaa caaacaaaag aactaaacaa
    12181 aaaggaaaaa caacagcgca gcagttgttt aaattccctc aaaaaagacc accgcgatag
    12241 cggcttatat aaaaccctca caaaattgtt cttcaaagat aaatacaaac gatatgaaaa
    12301 caaaatagag acaacaattt caattcaaga tataaaaaaa tcagacaatc aacattatag
    12361 aattttattg tcaaacatag atggagtaaa tactgaagac tggtggaaag gggtaataaa
    12421 caaagaaaaa actttcatta cagatcattt atggttacat gtaaaattat acgaccaacc
    12481 aaacaatata tatgttgtta aaatattcct tgaaaacaca ttactttttc cacacacaaa
    12541 atttgctttt tcaaaggaaa gcttaaatat ggcctgcttg ttccaaacag aaataaaggt
    12601 tgaaacacct tatttacagc cgtttaatga ttataaaaat aatttgtaca aatcagatga
    12661 agacagaatg ttggattttc tttatcgtga aaagttatct tatggcattg gccataatac
    12721 cgcctgtact tgggaaaatt gtgatgatga tactcaaaaa ccgcgatggg ttagttcaac
    12781 ctttttgcct cagttcaatg taaaaaatca aagtaccaat atctcaagtg tggataaaaa
    12841 atgtttagaa ataaaagaac tatctgaatt caataataat aaaaaaacca taatttcaaa
    12901 tcttgaaaag ttagctcaat cttattctaa atggattgat gaagaagaaa aaataaaact
    12961 attaaatgag catgaccgat tattagggct aaacaatata aagaaatgta gaacaataca
    13021 ggcaagaatg cagagaggaa ttgaattgtt agaaacaaat tctatggcgt ttcatgcatt
    13081 tcaattagca aacacggcaa tttatttgca aatgttccaa aaccaatggc attttagcaa
    13141 gaaaaaagat gggttcgaac cttttgagat aatagaaaat cctcaacttt cttatcaaga
    13201 atatgctaca aaagattatc ccgacccaaa caataccccc agatggcgtc catttcaatt
    13261 agcatttatt ttgcagtgtt tgccgtcatt cattgacgaa aattcaaatg acagaaattt
    13321 ggttgatttg ctttatttcc caactggtgg aggaaaaaca gaagcgtatt tagctttgtc
    13381 ggcttttcta attttttgga ggagattgaa ttatccggac agttatggag gcgtaaatat
    13441 cattattcgc tatacattgc gtttgctttc cgcacaacaa tttgaaagag caactaaact
    13501 gattttggct tgtgaattta ttcgtaaaaa ccatacggat ttaggaaatg aaaggatttc
    13561 aattggtttt tgggtaggtg gttctacaat tccaaacaaa attaacaatg atcaaaattg
    13621 tgcagagcaa gaacttcaga aagtacaaga tcgattaaat aaaaatcagc cctccataaa
    13681 tccgtttcaa ctatctaatt gtcaatggtg caatacaaaa atcataggaa aaacacaacc
    13741 caatgtcaat ccttcaatag gacacagaat ttataaaaaa agtttgaaaa gttattgttt
    13801 gaacaaaaat tgtttttttt ctgaaaataa tgaaggatta cctattgtgt tggtggacga
    13861 agatatttac aaaaatcctc ccaccgtact gtttgcgact gttgataaat ttgcgcaatt
    13921 ggcttggagg gaagaagcga caagtttatt taattataat ggaaacagaa aaccggagtt
    13981 gataatccag gatgaattgc atttactgaa tggcccttta ggcagtttag taggactatt
    14041 tgagaatgtt attttatcat tatgcactac ggataatcaa aaaccaaaaa tcattgcatc
    14101 aactgccaca ataaaaaatg tagaggagca agtaaaaggt ttgtataatc gtccagttca
    14161 agtatttcca cagtatgcca ccaatgcaga tgatacgttt ttttcaaaga caatattgga
    14221 aagcaaacgg agatatgtag gtgttttacc aacaggtaga actcaaacaa tgacaagttt
    14281 acgtttaaat gcagctttgc tgtttgctcg tttggaaata tggaaagaca gtgtcgataa
    14341 aactgaagct gatcaatttt ggaccattgt ttcctacttt aaaagtttaa aacatcttgg
    14401 tcgattctca aataaaatta ttgcagaatt gatacctgaa ataaagcaat tacaggttag
    14461 atatttgatg aatgtatttc cgttcaacaa caattacagc aaactacctt accgaaattt
    14521 agaactcaca agccgaatac ctaacgaaag aataaaaaag aatttggata aattaaatat
    14581 ttcctttgac ggtaatttgg aaaaatacca atctttcgat atagttttag caacgaatat
    14641 gataagtgta ggtttggatg tcgaacgatt gaatataatg ctgatgaatg gaatgccccc
    14701 taacacagca gaatacattc aggcgagcag tcgtgtggcc cgaaaaaatg agggtgtagt
    14761 ctttagctta ttcgatccaa acaacacaag agatttatct tatttcgaag gttttatacc
    14821 atttcataaa acattttaca aacaagtcga gccaataagc gtaactcctt ttgctgaaaa
    14881 tgctttggat aaaatgctat ttacctcaat gatagcttat ttcaggcaca aaatgggcta
    14941 tagtgccaac aatatggcaa cagctttaaa tgaaaatgga aataagcaaa aacttatgag
    15001 cgaatttaat caacttttta acaatcactc ctttgccgat gaagaagata aacaagtgat
    15061 atcaacaata ataaacgaat taatacaaca atgggataat aaaattcaaa atagtccaaa
    15121 agatagtttg ttttactatg gtgggaagca tgagactgaa agaaagaaaa acttactaaa
    15181 gtccgttcaa gagaaagaaa aaacggacga taaattaatc ggcatgcaat caatgcgaag
    15241 tgttgaacca agtgtaacca ttaaaattaa aaaattttga tctatggctg aaaaaataaa
    15301 tcacggaaga agtaaaatgg tatcttctta tggcggagta ggctctgtta ttgatactcc
    15361 cgatgcttcc atcattatag aaacatttga taattgggga taccataatt atgacgacca
    15421 agagaagcat aaacatatta ttttagacga ccggttagta tttcgcttgc gaactcgttt
    15481 tcctaacttg aaatacttga tgtccattcc tattgaaaag gatggcaaag tacaacccca
    15541 ggcaaactat tttccaaaat ggttttattg cccacactgt gatcgtttta tgcatataaa
    15601 tgattggaaa aaacattggg attcgggaaa aggggaattt gacttgcaat gttttgatcc
    15661 caaatgcaaa gggcaacatt tagaacaagt acgttttgtt atgacatgcg aaaatggtca
    15721 tattcaagat ttaccatggg aatattggaa taatcgcgaa caaattgttg aaaatattga
    15781 actcccatta aatgaaactg aaaatattga agatgacgat attgaagata ataatgaaca
    15841 agagagtcaa tccaaaattc gattaatata taaaaaatgt tgcccggaac aagaattata
    15901 ttatagtgta agcaatgaaa acaccgattt atcaggcatt catataaaat gtaaaaattg
    15961 cggaaaatct gagacattaa aaggtatatt tggattttct caaaattgtg gtggaagaaa
    16021 atattggctt ggtcttaaaa atgggcaata tcaaggagag ccatgtaata aaagagcaaa
    16081 agttaaaata aaaaccagta atagcattta ttatgccaac accttgagta gcttgtggat
    16141 acctgaaaaa caaattcttg atttatctgc cgaattgaga acggaaattg atggcattgt
    16201 taacgatcaa gattttgaac aaaaagactt agaaaaattt gcacgacgaa aaggaattgc
    16261 aatagaaatt atcaatcagt atttagacaa tactgatact gcatatattc ctgagattgt
    16321 atatcgtcaa gccgaatata actatttctt aaacaaacaa caacccgaag atcaagtaat
    16381 caaatttagt caaattaatg tgagtggaaa acttaatggt tttgaaaaat tagtaaaaat
    16441 aaatcgcttg aaaaaaatta ccgttcaaac ttcttttact cgaaacgaac caattgatgc
    16501 tgatagtatt ttgatagatc cagagagtgg caattatcaa tatatggtta agcctcaatc
    16561 tgtttctaaa aacagctttg aaactcactt attgcctgcc attgaaaatt acggcgaggg
    16621 cattttattt gtcttagacg agcaaaaact caaagaatgg gggaaaaatg acattatcac
    16681 agaaagagta aaaaacattc aaactcacgc cgaagattct gattggcaat ttcacaagat
    16741 tgaagcagaa aatctgagtg ctcggaaaat tctaatccat acactttctc atttgcttat
    16801 tcgggaattt gaatatgttt gtggttatcc gatgtcttct ttgcaagaac gattatatgt
    16861 aagtgaaaac atgaatggtt ttctgatttc cgcttacgat ggaacagatg gatatttggg
    16921 cggactggca aagctgtgca acaacctcga aaatcttcaa gctataatta caaatgctat
    16981 acaacgagcc aaagaatgtt cccttgatcc aatttgttat gaaagcgaag ggcaaggtgt
    17041 agcaatgtta aatttagccg cttgtcattc ttgtacatta atacccgata caagctgtga
    17101 attgagtaat ctatttttgg atagaagatt acttattgat gatgaatttg gatattttat
    17161 ataatagaaa aattttctcc ccgaatc
//
LOCUS       DAOSIB010000044        17141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_3533_length_17141_cov_11.091303, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000044 DAOSIB010000000
VERSION     DAOSIB010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17141
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3533_length_17141_cov_11.091303"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..1182
                     /locus_tag="PLD44_05335"
     CDS             160..1182
                     /locus_tag="PLD44_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF98790.1"
                     /translation="MQPLIEAVDKEVLKRELTPEKFLRPTNKSDNEIYVITAHNSPNV
                     MLEIGRLREMAFRSWGGGTGKEIDVDAYDYSEKPYYQLIVWDPEENEIVGGYRFLFGK
                     DVPLNEKGEPEFVMSHLFKYSKKFINEYLPYTIELGRAFIQPAYQTSQKGSKSIFALD
                     NLWDGLGALIHLAKEARYFIGKVTMYHAYPAAARELIYEYMFRFFPDKEKLILPRNPV
                     TISNNIKILSNKLFNEKDKEANYKILQKMVRKENVNIPPLFNAYIGLTNTLRMFGSAI
                     DPNFSSVYETGIMLTMSDIVETKRKRYIEPYIEYLKNVFSNGTFSKINAYESHCRNIY
                     NFFQFE"
     gene            complement(1195..2994)
                     /locus_tag="PLD44_05340"
     CDS             complement(1195..2994)
                     /locus_tag="PLD44_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOF98791.1"
                     /translation="MCGIAMIRLLKPLDYYQKKYGTWEYGLQKMYLLMEKQHNRGQEG
                     AGIATVKLDMPPGEEYISRKRIEGKNAIQEIFVDIDKNHSLAPEDYSSDAAKAKMFCP
                     FAGELYLGHLRYSTSGKSGLSYIHPFLRRHNWKSRTLVLAGNFNLTNVDEIFQSLTAQ
                     GQHPRDKSDTFLMLESLGDKLDREVQREYEIIKQRYSNDQVITRKIEENLDIASFIRK
                     SEHIWDGGYVICGMLGHGDAFLFRDPKGIRTVFYYADDEIIVAASERPVIQTAINVKA
                     EQVHELKPGEALIIKKNGSMHFETIREADPEPKPCSFERIYFSRGSDKDIYQERKKLG
                     GFLSEAILKAVDQDIDNTVFSFIPNTAEVAFYGMTDGIRTLTGKVPRIEKVLLKDIKL
                     RTFISQNKERNDLAAHVYDTTYGSIRPEKKDNLVVIDDSIVRGTTLKQSILNILNRLE
                     PKKIVIVSSSPQVRFPDYYGIDMARMNEFCAFRAAVQLLKDSGRQKIMDDIYEKCKQQ
                     ENWPKEKVSNYVKEIYAPFTQEEISSQIAKMLTPEEVHCPVELVYQTIDNLHKACPQH
                     KGDWYFTGDYPTPGGNRMVNKAFIDYYEGKDKN"
     gene            complement(3048..4889)
                     /gene="glmS"
                     /locus_tag="PLD44_05345"
     CDS             complement(3048..4889)
                     /gene="glmS"
                     /locus_tag="PLD44_05345"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOF98792.1"
                     /translation="MCGIVGYMGYREAYPILIKGLQRLEYRGYDSAGIALLNDHKLTV
                     YKTRGKVSDLIHSVENKDISGTVGIAHTRWATHGEPNNANAHPHCSQSEELAIVHNGI
                     IENYAVLKQGLIEHGYTFSSETDTEVLVQLIEYVKRKNNVDLPTAVQLALNQIVGAYA
                     IAVIEKGHPDVIVAARKGSPLVVGIGDNEYFLASDATPIIEFTHKVVYVGEEEIVVLQ
                     RGKDLKIKTIGNVEKTPEIKELEMTLSQLEKGGYPHFMLKEIFEQPKTLSDSMRGRIN
                     LEENNISLSGFIDHKEKFLNAKRIIITACGTSWHAGLIGMYAIEEFARIPVEVEYSSE
                     FRYRKPVINKDDLVIAISQSGETADTLAAVQLAKSHGAFIFGICNVVGSSIARNTDSG
                     CYTHVGPEIGVASTKAFTAQVTVLIMLALLIGKEKGTIENEQYLKIVGELAKIPEKIS
                     QILKQNDVIAEFSKTFTYAQNFIYLGRGYNYPVALEGALKLKEISYIHAEGLPAAEMK
                     HGPIALISQEMPVVVIAPSAGKYEKVVSNIQEIKARKGRIISIVTEGDVIVKNLSDYI
                     ISIPETVECLVPLLAVIPLQLLAYHIAVVKGCDVDQPRNLAKSVTVE"
     gene            complement(4926..6152)
                     /locus_tag="PLD44_05350"
     CDS             complement(4926..6152)
                     /locus_tag="PLD44_05350"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOF98793.1"
                     /translation="MALANENYLKIPESRFSDEIEKRVNAFKVLHPTTKLIRLGIGDV
                     TRPLPAESVFAIHKAADEMLRTETFKGYGPLQGYDFLVDKILKNYRSSGIVLDKESIF
                     INHGAKPDIGNIGHILGRDNIIAIAEPVYPVYENAAIMSGRGGEMNEEEKWSNIVYLH
                     CSKENNFVPELPDERVDIIYLCNPNNPTGTVINKTELKKWVDYAIENQSIIIYDGAYH
                     AYIKEPDIPHSIYEVKGAKKVALEIRSYSKTAGFTGLRCGYTVFPKELMVYTRTGEEI
                     PLLKLWYRRNLNYSNGVSYVVQRAAEALYSRKGRMEIQELVNYYLENAKKIRKELLEL
                     GWEVYGGINSPYVWFKTPHNEPSWKFFQELLYNYNIIGMPGIIFGKSGDGYMRFSGFC
                     SHEDTEEAIKRIKSNY"
     gene            6655..7200
                     /locus_tag="PLD44_05355"
     CDS             6655..7200
                     /locus_tag="PLD44_05355"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOF98794.1"
                     /translation="MNQPTSAIISEELIDEIIGKYQNKPGFLLSVLEEIQEKNPLKYL
                     PAETLQTVAAKLNLPYSRVYGVVTFYSFFNLRPQGKHSIVVCRGTACHTKGSKQLLNE
                     IGHSFGMNHQDENSEASFTTADNQFTVRTVACFGQCAQSPVVEVDGKIYSYVNSQKLL
                     KILENVKKNGVQKNFSVNTSR"
     gene            7219..>7694
                     /locus_tag="PLD44_05360"
     CDS             7219..>7694
                     /locus_tag="PLD44_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOF98795.1"
                     /translation="MTVAELELLKNKNIERILPSVPMFLVGMSTCGLGNGAESVYRIL
                     EKEIELRHVNFKLKQTGCFGYCAEEPIVMMYQPQKPLVVYSRVNEKDISAIIDGIIKS
                     KIYRKKILCRIDFWDHLTSKIEYGKDYEDVPHWDEISFFKGQKKIVLRDAGFIDPES"
     assembly_gap    7695..7794
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7795..10059
                     /locus_tag="PLD44_05365"
     CDS             <7795..10059
                     /locus_tag="PLD44_05365"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF98796.1"
                     /translation="NILNTGFNFDIQLVEGAGAFVCGEETALIASIEGKAGRPMPRPP
                     YPAERGLYGKPTNINNVETWCNIPVIIEKGSDWFTKTGTERSAGTKVFSLVGKIKNTG
                     LVELPLGSTLEKFIFEIGEGTGNNKRIKAVQTGGPSGGCIPVEYFSTPVDYESLSSLG
                     AIMGSGGMVVMDQDNCMVDVAKYFLEFSANESCGKCTPCREGLSQALSIMNKISKGEG
                     NFQDIETLELLTRTIKDTAICGLGQTAPNPILTTLRYFKNEYEEHIQEKRCHAGTCES
                     LFLALCENSCPLHMNIPGYIELVKEDRIEEAFELTLRDNPIPGTIGRICHFHCQMRCR
                     REMVDEPVAQGEIHRYLADTMYQLGKENEIYNRLIAEKLPSSGKKIAIVGAGPAGITA
                     AFYLVRLGHEVTVFDAMPEAGGVLRYGIPQYRLPKSVLKKELKLIKKLGVKFVFNTRI
                     GKDIFLQDLKNNFNAVYLAFGAWKDIDLNIPGENAKGVFAGTDLLKELSLDKTPRIGQ
                     KIVVIGGGNVAIDAARSLWRLGKDVTVVYRREKIDMPANKDEIAEAEAEQIHFRFLSA
                     PHEILTDNSGRVNALKIEIMTTGPVDTSGRRKPIATGKFEEIPCDTVVLAVGERVDSA
                     PFVAEGLKVTKDGRLIVHPFTFQTSEPNIFAGGDAVMGPATAAEAMGMAKRAAEAIDW
                     ELMKEKRFHLLFKKFEYRNVIPTDIKKSPKNVSAKIQVKERIGNFHEVSTGYTGEQAR
                     DEVNRCLRCDVKVN"
     gene            10151..11908
                     /locus_tag="PLD44_05370"
     CDS             10151..11908
                     /locus_tag="PLD44_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOF98797.1"
                     /translation="MKITINHQPVDVKDGITIMEACQSVGIDIPSLCFLKDISSNASC
                     GICVVEVKGARSLLRSCITKVAEGMEILTTSPRVLEARKVNVELLLANHPQDCLQCDR
                     NLNCELQRLSFELGIKERHFVRTRKKYLPKDETSLSLVRDPEKCILCGRCVAVCSDMQ
                     SVNAIDFSGRGLNTSISTFFDKGLGATYCTNCGQCSLVCPTGAITEKTSVPQVWKEIL
                     NPEKMVLIQTAPAVRVGIGEAMGMPYGSLVTGQMVAGLKRLGFSKVFDTNFAADLTIM
                     EEANELMQRIRQNGVLPMITSCSPGWIKFAEHFFPSSLAHLSSCKSPQQMFGAIAKTF
                     YAEQTGIDPRKIVVVSVMPCTAKKYEARRPEMDDAFHYWKEKLQLSEEEHFFDVDYAL
                     TTRELARMFKESGIDFQHLPEEDFDQPLGVSTGAGTIFGATGGVMEAALRTAYEVMTG
                     RQLKDVEFLSVRGMKGIKEAEIDLDGRKIKVAVAHTLKNARFLLEQIRAGISPYTFVE
                     IMTCPGGCLGGGGQPIPTNSEIREKRAASIYREDERKQIRKSHENPEIQYLYEHFLEK
                     PLSEKSHHLLHTHYVERGC"
     gene            12014..12490
                     /locus_tag="PLD44_05375"
     CDS             12014..12490
                     /locus_tag="PLD44_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HOF98798.1"
                     /translation="MKTIDCTKLNAEGAKKVVDALQQLLADYQIFYTNLRGFHWNLKG
                     KDFFINHAKFEELYENVAEKVDEIAERILMLGGTPAHNFSDYLKTAKLKETGLVTDGD
                     TGLKNVLESYAYFIERERNIIKLAEAIDDEATVAQMSDYIKEQEKMVWMLVSYFEK"
     gene            12682..13755
                     /locus_tag="PLD44_05380"
     CDS             12682..13755
                     /locus_tag="PLD44_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOF98799.1"
                     /translation="MEQNYCIIMAGGIGSRFWPFSRKNRPKQFLDFFGTGRSLLQLTY
                     DRFRKIIPAENILIVSNELYRDLILEQLPEINDNQLLLEPVRRNTAPCIAYAIYKIKS
                     ITENANIIVSPSDHLILKESEFLETIRQGLEFVSKNDNLLTLGIKPSRPETGYGYIQI
                     EFGESNLRKVKTFTEKPNAELAKLFFESGEFFWNSGIFLWNLKTILAAFDFHLSEISQ
                     KFKQGLKYFNSTEEKKYIADIFHTCPNISIDYGILEKASNVFVLCSDFGWSDLGTWGS
                     LYDISPKDEKENVTLKCKTLYYDSHRNIVALPSGKLAVIQQLNDYIVADSDNVLLICK
                     REDEQQIRQFVKDASILYDGEFD"
     gene            13785..14069
                     /locus_tag="PLD44_05385"
     CDS             13785..14069
                     /locus_tag="PLD44_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98800.1"
                     /translation="MTPSFFNIYKPRKYDYRYIYYDPLKEAIKEREKQMAKENGEQPQ
                     QEYKPNLHRGSFREEANRMRGKKSKELRRSNRNVLIILIILLIIAYYLLK"
     gene            14085..15920
                     /gene="mutL"
                     /locus_tag="PLD44_05390"
     CDS             14085..15920
                     /gene="mutL"
                     /locus_tag="PLD44_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOF98801.1"
                     /translation="MSDIIHLLPDSVANQIAAGEVIQRPASVLKELIENSIDANANNI
                     IIQIKNAGRTLIQVTDNGKGMSETDARLAFERHATSKISNVSDIYALQSFGFRGEALA
                     SIAAVAQVELQTRRKEDELGTLIEIEGSKVIRQESVLCEEGTTIRVKNLFFNVPARRR
                     FLKSDNVEKIHLLNEFYRIALVYPDIRFSFFDDKEEIFKLLPSPLKLRIENIFGKGTK
                     RKWEQQLLPIETNTPLVKIEGYVGRPEFALKSAYQYFFVNARYMRHPYFHKAVISAYS
                     QMIRPEENPNYFIYLYVAPETIDVNIHPAKTEIKFENEQAIWSILLATVRETLGKYNV
                     IPSINFNQEDAIDLPIIGNNEKIVPPHTVFNPNFNPFENTLVNKRPPFNWETLYRNFE
                     KKEESKPTSFQWIENDITAYASSASSIIPEETLDSSHFLQLKTKYILTAVKSGLLVIH
                     QHKAHVRILYDLYLSQIKHQKSSSQQLLFPEILELSEHEMLYFSSIKNDLSNVGFLFK
                     SVGKNAFEIFGIPSSLDKINIQSFIHELIEKFKVKQSISEEDLHQQIALSMAETSAIK
                     TGQKLTQEEMTDLVNRLFATEKPSFSPQGEKILSIIPMEEFDSQF"
     gene            16103..16507
                     /locus_tag="PLD44_05395"
     CDS             16103..16507
                     /locus_tag="PLD44_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HOF98802.1"
                     /translation="MKLIDEKLLNELTRQAKENARLRVNYNLHEHLEDKVQRLLNAME
                     PGTKLPIHRHRHAAETYFLIRGSILVKYYDDKGNLTEETELNVKEGKYGIHIPAGQFH
                     TLEVLESGTIIFEVKEGPYQPIGENDILKSDT"
     gene            complement(16553..>17141)
                     /locus_tag="PLD44_05400"
     CDS             complement(16553..>17141)
                     /locus_tag="PLD44_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOF98803.1"
                     /translation="FSITVFVMDNVTIILIGIGLAMDCLAVAISKGISVKKFYYSSTL
                     FMAFLFGLFQAGMPLIGYYAGKSFVSFISKSDHWIAFGFLTLIGGRMLYEGLKNHQLA
                     NNGESNPFHWLKILSLAVATSIDALATGIIFVPYPDVIWKAIFIIGVISFLFAIVGMF
                     IGVRFGKRFHIKVEALGGIILMALGIKILIEHTCA"
BASE COUNT         5338 a         3231 c         3293 g         5179 t
ORIGIN      
        1 tttcatacaa atgtaaaaca aatattacat gcaaaatttg aacagacgga agagaaagag
       61 tacttttgca acaagaaaat tgttcaatcc aaattttaaa atagaaaaca ggtttgaata
      121 attgctcaat tctctaatat ttttgttttt cattctgtta tgcaaccact tattgaagca
      181 gtcgataaag aagttctgaa aagagaactt actcccgaaa aatttttacg ccctactaat
      241 aaatcagata atgaaattta tgtaattact gcacacaatt ctcctaacgt aatgttggaa
      301 atcggtcggt tgcgcgaaat ggcttttcgt tcgtggggtg ggggaaccgg caaggaaatt
      361 gatgtggatg cttatgatta ttcggaaaaa ccctattatc aactgattgt ttgggacccg
      421 gaagaaaatg aaattgtggg cggttaccgt tttttatttg gaaaggatgt tccgttaaat
      481 gaaaaaggag aacccgaatt tgtaatgtcg catcttttca aatacagtaa aaaatttatc
      541 aacgaatatc tgccttatac tatcgaatta ggtagagcat ttattcagcc agcctatcaa
      601 acctctcaaa aaggatcaaa gagcatattt gcactcgata atttgtggga tgggttggga
      661 gctcttattc atttggctaa ggaagcacga tattttatag gaaaagttac catgtatcat
      721 gcttatcccg ctgctgcaag ggagttgatt tacgaatata tgtttcgttt tttccccgac
      781 aaagaaaaac tgattttacc acgaaatccg gtaaccatta gcaacaatat taaaattttg
      841 tcaaacaaac tttttaacga gaaagacaaa gaggcaaatt acaaaatttt acaaaagatg
      901 gtgagaaagg aaaacgtaaa tattcctccg cttttcaatg cttatatcgg cctgacaaat
      961 acactacgta tgtttggttc tgccattgat ccaaacttca gttcggttta cgaaaccgga
     1021 attatgctga ccatgtcaga tattgtggaa actaaacgca aaagatatat tgaaccttac
     1081 attgagtatt tgaaaaatgt gttttcaaac ggaacgtttt ccaaaattaa cgcttacgaa
     1141 tctcactgca gaaacattta taactttttt cagttcgagt aactgctaaa atatttaatt
     1201 tttatctttt ccttcgtaat agtcgataaa agccttgtta accattctgt tgccgcccgg
     1261 cgtgggataa tcgccagtaa aataccagtc gcctttgtgc tgggggcaag ctttgtgcaa
     1321 attatcgatg gtttgataaa ccagctctac cggacaatgc acctcttcgg gagtaagcat
     1381 tttggcaatt tgagacgaaa tttcttcctg cgtgaaagga gcgtaaattt ccttcacata
     1441 attgctaact ttttctttcg gccagttttc ctgttgcttg catttttcgt aaatatcgtc
     1501 cataattttc tgcctgccgc tgtctttaag caattgaact gcagctctga aagcacaaaa
     1561 ttcgttcata cgggccatgt cgataccgta ataatcgggg aaacgtactt gcggagaaga
     1621 ggacacaatc acgatttttt tgggttccaa tcgattcaat atatttaaaa tactttgttt
     1681 gagcgttgtt ccccgaacga tggaatcgtc aatcactacc aaattgtctt ttttctcggg
     1741 tcggatggag ccataggtag tatcgtaaac atgagccgcc aaatcgtttc tttctttgtt
     1801 ttgcgaaata aaggtgcgca atttgatgtc tttcagcagc actttttcga ttcgtggaac
     1861 tttgcccgtg agagtgcgaa tgccatccgt cattccgtaa aaagccactt cggccgtatt
     1921 gggaataaac gaaaacacgg tattatcaat atcctgatcg actgctttta aaatagcttc
     1981 acttaaaaat cctcccaatt tttttctttc ctgataaata tctttgtcgc ttccacgtga
     2041 aaaatagatt ctttcaaaag aacagggttt tggttcggga tctgcctcac gaatggtttc
     2101 gaaatgcatc gacccgtttt tcttgataat aagtgcttct ccgggtttta attcatgtac
     2161 ctgctctgct ttcacattaa tggctgtttg aatgaccgga cgttcggaag cagcaacaat
     2221 aatttcatca tcggcgtagt aaaatacggt cctaattcct ttggggtcgc ggaaaaggaa
     2281 ggcatcgccg tgtcccaaca tcccacaaat aacgtaacct ccatcccaaa tgtgttccga
     2341 ctttcggata aaagaagcaa tgtcgagatt ttcttcaatt tttcgggtta tcacctgatc
     2401 attagagtag cgttgtttaa taatttcata ttcacgttgc acttctctgt cgagtttgtc
     2461 gccgagcgat tccagcatca gaaaagtatc cgatttgtcg cgtggatgtt gtccctgtgc
     2521 cgttagcgac tgaaaaattt catcaacatt ggtcagatta aaatttccgg ccaacaccaa
     2581 tgttcgcgat ttccaattgt gtctgcgcag gaacggatga atatatgaca atccgctttt
     2641 cccgcttgtg ctgtagcgca aatgtcccaa ataaagttct ccggcaaacg ggcaaaacat
     2701 ttttgctttg gcagcatccg aactgtaatc ttcgggcgcc agcgagtgat ttttatctat
     2761 atcgacaaaa atttcctgaa tggcattttt tccttctatt cttttacgcg aaatatattc
     2821 ttctccgggc ggcatatcca attttacggt ggcgattcct gcaccttcct gaccccgatt
     2881 gtgctgcttc tccataagaa gatacatttt ttgcaaaccg tattcccaag taccgtattt
     2941 tttctggtaa taatccaatg gcttcaatag ccttatcatt gcgattccgc acataataaa
     3001 tttcttattt tttaaaagag attataagat ttttgaaact caatatttta ttctacagtt
     3061 actgatttcg ccagatttcg gggttggtcc acgtcacaac cttttaccac agcaatgtga
     3121 taggccaaaa gttgcaacgg aatcacagcc agcaacggaa ccaagcattc tacggtttcg
     3181 ggaatggaaa taatgtaatc cgaaagattt tttacaatta catcgccttc cgtaacgata
     3241 gaaataattc taccttttcg tgcttttatt tcctgaatgt tgctgacaac tttttcgtat
     3301 tttcctgcac tgggtgctat tacaacaacc ggcatttcct gactgatcaa ggcaattggt
     3361 ccatgtttca tttcagctgc cggcagtcct tcggcatgaa tataggaaat ttccttcaat
     3421 tttaaagctc cttccaaagc taccgggtaa ttataacctc tacccaaata aataaaattt
     3481 tgcgcatagg tgaatgtttt ggaaaattcg gcaatgacat cattttgctt taaaatttga
     3541 ctgatttttt cgggaatttt tgccagttct ccaacaattt tcaggtattg ttcattttca
     3601 attgttccct tttcctttcc gataagcaat gccagcataa ttaaaactgt aacctgagct
     3661 gtaaaagctt tggtggacgc tacaccaatt tcggggccaa cgtgtgtata acagcccgaa
     3721 tcggtattgc gtgcaatgga tgagcccacc acgttgcata ttccgaaaat aaatgctccg
     3781 tgcgatttgg ccaattggac ggctgccagt gtgtcggcag tttcgcccga ctgagatatg
     3841 gctatcacca aatcatcctt gttgataacc ggcttgcggt atctgaattc cgaagaatat
     3901 tcaacttcaa ccggaattct ggcaaattct tcaatggcat acattcctat taagccggca
     3961 tgccatgatg ttccacaagc ggttataata atccgtttgg cattcaagaa tttttccttg
     4021 tggtcgataa atccagaaag actgatattg ttctcttcca aattgatgcg ccctctcatg
     4081 ctgtcgctca aagttttggg ctgctcgaaa atttctttca gcataaaatg tggataaccg
     4141 cctttttcga gctgactgag agtcatttcg agttctttga tttcgggagt tttttctaca
     4201 ttcccaatgg ttttaatttt taagtctttt ccgcgttgca aaacgacaat ttcctcttct
     4261 ccaacataca ccaccttatg cgtaaattca atgatgggag tggcatccga agccagaaaa
     4321 tattcgttat cgccaattcc cacaacgagc ggactgcctt ttcgagctgc cacaattaca
     4381 tcgggatgac ctttttcgat aacggcaatc gcataagctc ctacaatttg attcaatgcc
     4441 aattgcactg cagtaggcaa atccacgtta tttttccgtt ttacgtattc tatgagttga
     4501 accagcactt cggtatcggt ttcgctcgaa aaagtatatc catgctcgat aagtccctgt
     4561 ttcaaaacgg catagttttc aattattccg ttgtgtacaa tggccagttc ctcagactga
     4621 gaacaatgtg gatgtgcatt tgcattgttg ggttcaccat gtgtcgccca tcttgtatga
     4681 gcgataccga cagttccact gatgtctttg ttttcgacag aatgaatcaa gtcggaaact
     4741 tttcctcttg ttttataaac agttaattta tggtcgttaa gcaatgcaat gccggcgctg
     4801 tcgtagccac gatattcgag acgctgcaag ccttttatta aaatgggata agcctcgcga
     4861 taacccatgt aaccaacaat accacacata aagttaatga tttaagattt ttcttagata
     4921 attttttaat aatttgattt gattcgttta atggcctcct ctgtatcttc atgactacaa
     4981 aatccggaaa aacgcatata accatcaccc gattttccaa aaataatacc gggcattcca
     5041 ataatgttgt aattgtataa aagttcctga aaaaatttcc acgaaggttc gttatgaggc
     5101 gttttaaacc aaacatacgg agaattaatg cctccataaa cttcccagcc aagttctaaa
     5161 agttctttac gtatcttttt tgcattttcc aaatagtaat tgaccagttc ttgaatttcc
     5221 attcttccct tacgactgta caaggcttcg gcagcacgct gaactacata agatacacca
     5281 ttgctgtaat tcagatttct gcgataccaa agtttcaaca acggaatttc ctcgcccgtc
     5341 cgggtataaa ccatcaattc tttcggaaaa accgtgtaac cacatcgaag ccctgtaaaa
     5401 ccggctgttt ttgaataact tctgatttcc aaagcaacct ttttggcacc cttgacttcg
     5461 taaatgctgt gaggaatatc gggttctttg atataggcat gataagctcc atcatatata
     5521 ataatacttt ggttttcaat agcataatct acccattttt tcagttcggt tttgttgatt
     5581 acggttccgg tgggattatt ggggttgcaa agataaatga tgtctacgcg ttcgtcaggg
     5641 agttcgggta caaaattatt ttctttcgaa caatgcaggt aaacaatatt gctccatttt
     5701 tcttcctcat tcatttctcc gcctcttccg ctcataatgg cagcattttc gtaaaccgga
     5761 taaaccggct cagcaatggc aataatgtta tctcgtccca aaatgtggcc gatatttcca
     5821 atatcgggtt ttgcgccatg attaataaaa atactttcct tatcgaggac aatgcctgaa
     5881 gaacgataat ttttgagaat tttatcgacc aaaaaatcat acccttgcag tggcccgtaa
     5941 cctttgaaag tttccgtacg caacatttcg tctgcagctt tgtgaatggc aaaaacagat
     6001 tctgcgggta aagggcgtgt aacatctcca atccccaaac gaatcaattt ggtggttgga
     6061 tgcaaaactt tgaaggcatt cacccgcttt tctatttcgt ccgaaaaacg ggattcggga
     6121 atttttaagt aattttcatt tgctaatgcc attcaggaaa tgattaagca ggaatttttc
     6181 ctataaataa agcacaaaat tactgttttt tcattgatta tttttctttc aattcaaaat
     6241 ttcaacaata cttttcagta tttttgtgtt tttaatctcc ttttccaata aaaacaaact
     6301 tattcatcta attttatttc attatccaat ttttttcttt caaaatgtaa cttttatttt
     6361 taataaatct aagttcttcc acatgtttta aaaatgaaag aaaatattga tttttacttt
     6421 tattaaaaaa aattatttta taatcataac aaaataaaaa tgtgtcaatt aaagaaattt
     6481 ttccataaat aaatctaaaa aattgacaat aatcatttaa ttttatattt ttgtttatag
     6541 aaatattcaa agtatactga aacatcggat gattgaaatt atatattttt cttcagattt
     6601 tcaatgactt atttataaat tattagtcaa ataactttta aaaatgaagg ttttatgaac
     6661 cagccaactt ctgcaattat ttccgaagaa cttattgatg aaattattgg caaatatcaa
     6721 aataaacccg gatttttgct ttcggtgctg gaagagattc aggaaaaaaa tccattgaaa
     6781 tatttgcctg cagaaacgct tcagactgtt gccgcaaaac tcaatttgcc ctattcgcga
     6841 gtttatggcg ttgtaacttt ttattctttt ttcaatcttc gtccgcaggg aaaacattcc
     6901 attgtggtgt gcaggggaac agcctgtcat accaaaggtt cgaagcagct gcttaacgaa
     6961 atcggacatt ctttcggaat gaatcatcag gatgaaaatt ctgaagcttc ttttacaact
     7021 gccgacaatc agtttacagt gcgaacggta gcctgttttg gacagtgtgc acaatcgcct
     7081 gtggtagaag tggatgggaa aatctacagt tatgtaaatt ctcaaaagct gctgaaaatt
     7141 ctcgaaaatg ttaaaaagaa cggagttcaa aaaaatttct ctgtaaatac atctcgttaa
     7201 ccttaaaatg ttatggctat gacagtggca gaacttgaat tgttaaaaaa taaaaatatt
     7261 gaacggatat tgccttctgt tcccatgttt ctggttggaa tgagcacttg cggcttggga
     7321 aatggagcag aatcggtata tcggattttg gaaaaggaaa ttgaacttcg ccatgttaat
     7381 ttcaaactca aacaaaccgg ttgttttggt tattgtgcag aagaaccaat tgttatgatg
     7441 tatcaacctc aaaaaccatt ggtagtttac agccgcgtaa acgaaaagga catttctgcc
     7501 atcattgatg ggattattaa atcaaaaatt tatcgaaaaa aaattctttg tcgtatcgat
     7561 ttttgggatc atttgacttc taaaatcgag tatggaaaag attacgaaga cgttccacat
     7621 tgggacgaaa tttccttttt taaaggacag aaaaaaattg tgttgcgtga tgcaggtttc
     7681 atcgatccag aatcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaatata
     7801 ttaaataccg gctttaattt cgacattcaa ctggttgaag gagccggagc atttgtttgc
     7861 ggagaggaaa cggcattaat tgcttctatc gaagggaaag caggccgtcc catgcctcga
     7921 cctccttatc ctgccgaaag aggattgtac ggcaaaccga ccaatatcaa caacgttgaa
     7981 acatggtgca atatcccggt aattattgaa aagggaagcg attggtttac caaaacggga
     8041 acggaaagga gcgccggaac aaaagtattt tcgttggttg gaaaaattaa aaatacaggt
     8101 ttggtcgaac ttccgttagg ttcaactttg gaaaaattta tttttgaaat aggcgaaggt
     8161 acgggaaaca acaaacgtat caaagccgtt caaaccggtg gcccatcagg aggctgtatt
     8221 ccggtggaat atttcagtac gccggttgat tatgaatctt tatcaagtct gggtgctatc
     8281 atgggttcgg gcggaatggt agtaatggat caggataatt gcatggtgga tgtggcaaaa
     8341 tattttttgg aattcagtgc caacgaatca tgcggcaaat gtacgccttg ccgcgaagga
     8401 ctgtcacaag cgctttccat tatgaacaaa atatccaaag gagaagggaa ttttcaggac
     8461 atcgaaactt tggaattatt gacccgaacc atcaaggata ctgccatttg cggcttggga
     8521 caaaccgcgc ccaatcccat tcttactact ttgcgttatt ttaaaaacga gtacgaagaa
     8581 catattcaag aaaaacgttg ccatgccggt acctgtgaat cgttgtttct ggcactttgt
     8641 gaaaatagct gtccgttaca catgaacatt cccggctata tcgaattggt taaggaagac
     8701 cgtattgaag aagctttcga actcactttg cgcgataatc ccatacccgg taccattggc
     8761 agaatttgcc attttcactg tcagatgcgt tgtcgacgcg aaatggttga tgaaccggtt
     8821 gctcagggcg aaattcatcg atatttggcc gacaccatgt atcagttggg gaaggaaaat
     8881 gaaatttaca accggctgat tgccgaaaaa cttccttctt cgggtaaaaa aattgccatt
     8941 gttggtgccg gtccggcggg aattaccgct gcattttatt tggtgagact cggacatgaa
     9001 gttaccgttt tcgatgccat gcccgaagcc gggggtgttt tacgttacgg aatacctcaa
     9061 tatcgccttc ctaaatcggt actgaaaaaa gaactgaaac tgataaagaa attaggcgtt
     9121 aaatttgtat ttaatacgcg tatcgggaaa gacatttttt tgcaagattt gaaaaacaat
     9181 ttcaatgcgg tttatcttgc ttttggagca tggaaagaca ttgatttgaa tattccggga
     9241 gagaatgcca aaggagtttt tgccggaacg gatttattga aggaactttc gctggacaaa
     9301 acgcccagaa tcggacaaaa aattgttgtt attggcggag gcaatgtggc cattgatgca
     9361 gcaagaagtt tatggaggtt gggaaaagat gtaacggtgg tttatcgtag agaaaaaatt
     9421 gatatgcccg ccaataaaga cgaaattgcc gaagcggaag cagagcagat tcacttcaga
     9481 tttttgtctg ccccacatga aattctgacc gataattcag gaagagtcaa tgctttgaaa
     9541 attgaaatta tgactacagg gccggtcgat acttcgggca gaaggaaacc gattgcaacc
     9601 ggtaaatttg aagaaattcc ttgcgatacg gttgtgttgg ctgtgggaga acgtgttgat
     9661 tccgctcctt ttgtagcaga aggtctgaaa gttaccaaag acggaagatt aatagttcat
     9721 ccttttactt tccaaacttc tgagccaaat atttttgccg gaggagacgc cgtaatggga
     9781 ccggcaacgg ctgcagaagc tatgggaatg gccaagagag cagcagaagc catcgattgg
     9841 gagttaatga aggaaaagcg tttccatttg ttattcaaaa aatttgaata tcggaatgtg
     9901 attcccacag acattaaaaa atcgccgaaa aatgtttctg caaaaataca ggttaaggaa
     9961 cgaataggta attttcatga ggtttcgaca ggttacaccg gcgaacaagc tcgagatgaa
    10021 gttaaccgct gtttgcgttg cgacgtgaaa gtcaattgat tttcctgaat aattattttc
    10081 aaatttctgt tcttgaccta aattcaatga aacttaaaaa ataaccttat ttgaaaatta
    10141 taatcaatct atgaaaatca caattaatca ccaaccggtt gatgtaaaag atggaatcac
    10201 tataatggaa gcttgccaat ctgtcggcat tgacattcct tcgctttgtt ttctcaaaga
    10261 tatttccagc aatgcttctt gtggcatttg tgtggtagaa gtaaagggag cgcgcagttt
    10321 attgcgttcg tgcattacaa aagttgctga aggaatggaa attcttacca catcaccacg
    10381 tgtattggaa gcacgaaaag tcaacgttga gctgcttttg gccaatcatc cgcaagattg
    10441 cctgcaatgc gacagaaatt taaattgtga attgcaacgt ttatcctttg aattgggaat
    10501 taaagaaaga cattttgtac gtaccagaaa aaaatatctt cccaaagatg aaacttctct
    10561 ttcgctggtt cgcgatcccg aaaagtgtat tttgtgcggt cgatgcgttg ccgtttgtag
    10621 cgacatgcag tctgtgaatg ccattgattt ttcgggtaga ggattgaata cgagtatttc
    10681 gacttttttc gacaaaggat tgggtgcaac ctattgtacc aactgtggcc aatgttcgct
    10741 ggtttgtccc acaggagcca ttaccgaaaa aacatctgtt ccacaagttt ggaaagaaat
    10801 actcaatccg gagaaaatgg tacttattca gacagctccg gctgtgagag tagggattgg
    10861 cgaagcaatg ggcatgcctt atggaagttt ggtaaccgga caaatggttg ccggtctgaa
    10921 aagattgggt ttctcaaaag ttttcgatac caattttgca gccgatctta ccataatgga
    10981 agaagcaaac gaattaatgc aacgtattcg gcaaaatgga gttttaccca tgataacgtc
    11041 ctgttcgccc ggatggatta aatttgccga acattttttc ccttcatctc tcgctcactt
    11101 gtcttcctgc aaatcgcccc agcaaatgtt tggtgccata gccaaaactt tttatgccga
    11161 acagaccggt atcgatccgc gaaaaattgt cgttgtttcg gtaatgccgt gtacagcaaa
    11221 aaaatatgaa gcccgccgac ctgaaatgga tgatgcgttc cactattgga aagagaaatt
    11281 acaactttcg gaagaagagc acttttttga tgtcgattat gcactgacta cgcgagaatt
    11341 ggcaagaatg ttcaaagaaa gcggaataga tttccaacat ttaccagagg aagatttcga
    11401 tcagccgttg ggtgtttcta ccggagccgg aacaattttc ggagcaaccg gcggcgtcat
    11461 ggaagcggct ttaagaaccg cttatgaagt tatgacaggt cggcaactga aggatgttga
    11521 atttttgtca gtcagaggta tgaaaggtat taaagaggcg gaaattgatt tagacggaag
    11581 aaaaattaaa gttgccgtag ctcacacatt gaaaaatgca cgttttttgc ttgaacagat
    11641 tcgagcaggg atttctccct atacattcgt ggagattatg acttgtccgg gaggatgttt
    11701 gggaggtgga ggtcagccta ttcctacaaa ttccgaaata agggaaaaac gggcagcttc
    11761 catttatcgt gaagatgaac gaaaacaaat acgaaaatct cacgaaaatc ccgagataca
    11821 gtatctttac gaacattttt tggaaaaacc gttgagtgag aagtcacatc atttattgca
    11881 tactcattac gtagaaaggg gctgttgaat tatagatatt ttctattatt tcatttatgt
    11941 tttcagtttg atttattgtt ttaaacatat aattttgcct taaataatta atcaaaaaaa
    12001 ggaggtattt attatgaaaa caattgattg tacaaagctg aatgctgaag gagctaaaaa
    12061 agtggttgat gctttacaac aacttttggc cgattatcag attttttaca ccaatttgcg
    12121 tggatttcac tggaacttga aaggtaaaga ttttttcatc aaccatgcca aatttgaaga
    12181 attgtatgag aatgtagccg aaaaagtgga tgaaattgca gaacgcattt taatgttggg
    12241 tggaacgccg gctcataatt tcagtgatta tcttaaaaca gccaagttga aagaaaccgg
    12301 tttggtaact gatggcgaca caggattgaa aaacgtactc gaatcttacg cttattttat
    12361 tgaaagagaa agaaatatca ttaaattggc tgaggctata gacgatgagg ctacggtggc
    12421 tcaaatgagt gattatatta aggaacagga aaaaatggtt tggatgctgg tatcctattt
    12481 tgaaaaataa aggatgaacc atgtcaaaat taaaaaacgg ggttgatatt caatcaatcc
    12541 cgttttttcg ttcatcctga ttttttcgag catgttacca aatttctaca attattttgg
    12601 caaaaaaaga aaaacactgt atcttagtgg tgttattttg ttataagcat tttttaaaac
    12661 aaatttaaag tccaagtatt tatggagcaa aattattgca ttataatggc aggcggcatt
    12721 gggagccgtt tctggccttt tagcaggaaa aacaggccaa agcagtttct cgattttttt
    12781 ggtacgggaa ggtctttgct tcaattgact tatgaccgtt ttagaaaaat aatccccgca
    12841 gaaaacattt tgattgtctc caacgaactt tatcgcgatt tgatattgga acagcttccc
    12901 gaaatcaacg acaatcaatt gttgctcgaa ccggtcagaa gaaatacggc accatgtatt
    12961 gcttacgcca tttataaaat aaagtcgatt accgaaaacg caaatatcat tgtttcgcct
    13021 tccgatcatt tgatcttaaa agaatcagaa tttttggaga ctatacgtca ggggctggaa
    13081 tttgtttcca agaacgacaa tctgctgaca ttgggcataa agccgagtcg tcccgagaca
    13141 ggatacggtt acattcaaat agagtttgga gaatcaaatt tgagaaaagt aaaaactttt
    13201 accgagaaac caaatgcaga attagccaag ttgttttttg aatcgggtga gtttttttgg
    13261 aattcgggaa tttttttatg gaatttgaaa accattttgg cagcctttga ctttcatctt
    13321 tccgaaatat cgcaaaaatt caagcaaggg ttaaaatatt tcaattctac tgaagaaaaa
    13381 aaatatattg cagatatttt tcatacctgt cccaatattt ccatcgatta tggtatcttg
    13441 gaaaaagctt ccaatgtgtt tgttctttgt tccgattttg gatggtctga tttgggaact
    13501 tggggctcgc tgtacgacat atccccaaag gacgaaaaag agaatgttac ccttaaatgt
    13561 aaaactcttt actacgacag ccatcgtaat atcgtggcgt tgccttcagg gaagctggca
    13621 gttatacaac aacttaatga ttatattgtt gccgattcgg acaatgtatt acttatttgc
    13681 aagcgagaag atgaacaaca aattcgtcag tttgttaaag atgccagcat tctttacgat
    13741 ggagaattcg attgaattta tactttttaa aaccataaaa cattatgaca ccaagctttt
    13801 ttaacattta caagcctcgt aagtacgact atcgctacat ttattacgat cctttgaaag
    13861 aggcaattaa ggaacgtgag aaacaaatgg caaaagagaa tggagaacaa cctcagcaag
    13921 aatataaacc caatctgcat cgcggttcat ttcgagaaga agccaatagg atgagaggaa
    13981 aaaaatcaaa agaattacgt cgttcgaaca gaaatgttct tattattctg attattttgc
    14041 tgatcattgc ctattatctg ttgaaataaa atccaaatgc ctgcatgagt gatatcatac
    14101 atttattacc tgactcggtt gccaaccaga ttgcagccgg tgaagtaatt caaaggccag
    14161 cttctgtgct gaaagaatta attgaaaatt ccatagatgc aaatgccaac aacattatta
    14221 tccaaataaa aaatgcaggg cgcaccctga ttcaggttac agacaacgga aaaggaatga
    14281 gtgaaaccga tgctaggctg gctttcgaac gtcatgctac ctcaaaaata tcaaatgttt
    14341 cagatattta tgccttgcaa agttttggtt tcagaggcga agcgctggct tctattgctg
    14401 cggtggctca agtggaactt caaactcgtc ggaaagaaga cgaattggga acgctgattg
    14461 aaattgaagg ttcaaaagta attcgacagg aatcggtttt atgcgaagaa ggaacaacta
    14521 tcagagtgaa aaatttattt ttcaatgtac ctgcccgccg tcgtttcctc aaaagcgata
    14581 atgttgaaaa gattcatttg ctcaacgaat tttatcgaat tgctttagtt tatcccgata
    14641 tccggttttc ttttttcgac gataaagaag aaattttcaa actcttacct tctccactca
    14701 aacttcgtat tgaaaatatt tttggaaaag gcaccaaaag aaaatgggaa caacagctgc
    14761 tacccattga aaccaatacc ccattggtta aaattgaggg atatgtaggt cgccccgaat
    14821 ttgccttaaa atcggcttat caatattttt ttgtcaatgc tcgctatatg cggcatcctt
    14881 actttcacaa agcagtgatc agtgcataca gtcaaatgat tcgtccggag gaaaatccca
    14941 attattttat ctatttatat gttgctccag aaaccatcga tgtcaatatt catcctgcca
    15001 aaactgaaat aaaatttgaa aatgaacagg ctatctggtc tattcttttg gctactgtcc
    15061 gcgaaacgct tggtaaatat aatgttattc catctatcaa ctttaatcag gaggatgcta
    15121 tcgaccttcc cattattggt aataacgaaa aaatcgtacc accccacact gttttcaatc
    15181 caaatttcaa tccctttgaa aacacattgg ttaataaacg accaccattc aactgggaaa
    15241 cgctttacag aaattttgaa aaaaaagaag agagtaaacc aacttccttt caatggatag
    15301 aaaacgacat tacggcttat gcttcatccg cttcaagtat aattccagaa gagaccctcg
    15361 atagcagcca ttttttacaa ctcaaaacca aatatattct tacggctgta aaatcaggtt
    15421 tgttggtcat tcatcaacac aaggcgcatg ttcgtattct gtacgatctt tacctatcac
    15481 agatcaaaca tcaaaaaagt tcttctcaac aattgctttt ccctgaaatt ttagaacttt
    15541 ctgaacatga aatgctttat ttttcatcaa taaaaaatga tttgagcaat gtcggttttc
    15601 tattcaaatc cgtaggtaaa aacgctttcg aaattttcgg cataccaagc tcactagata
    15661 aaataaatat tcaatcattt atacatgaat tgatagaaaa attcaaagtc aaacaatcaa
    15721 tttccgagga agatcttcat caacaaattg ctttgtctat ggcagaaaca tctgcaatca
    15781 aaaccggtca aaaacttaca caagaagaaa tgactgacct cgtgaatcgt ttgtttgcca
    15841 ccgaaaaacc atctttttcc ccacaaggtg aaaaaatctt atccattatt ccaatggaag
    15901 aatttgatag ccaattttaa aactttcatt tttccgttta tttgttgcaa ttaaagaaag
    15961 gttaatatat aagttttcat ctgacaattt tttataatag gtggttcctt aaattcaata
    16021 gttcgttaaa aattaaatta tctgtcaaat aaatcgagta gaaataaaat tacgtggttg
    16081 tttgaatcaa aatttattac gcatgaaact gattgacgaa aaactattaa atgaacttac
    16141 ccgacaggcc aaagaaaatg cccgcttgcg agtcaattac aatctacatg aacatttgga
    16201 agataaggtt caacgtttgc tcaatgctat ggagccgggt accaagttac ctatccacag
    16261 acatcgacat gcggcggaaa catatttttt gatacgaggc agtatcttag tgaaatacta
    16321 tgatgacaag ggaaatctga cggaagaaac agaactgaac gtaaaggaag gaaaatatgg
    16381 aattcatata cctgccggtc aatttcatac actggaagtg ctggaaagcg gtaccatcat
    16441 ttttgaagta aaagaaggcc cttatcagcc aatcggagaa aatgacatac taaaatcaga
    16501 tacatgacaa tcgacgtgaa aaattcagaa aagtcaagaa aatatggttt tttcaggcac
    16561 aagtatgttc aataagaatt ttaataccca gtgccatcag aataattccg cccaaagcct
    16621 cgacctttat atgaaaacgt ttcccgaaac gaacgccaat aaacatgccc acaatggcaa
    16681 agaggaaact gatgactcca ataataaaaa tcgctttcca aatcacatcc ggataaggta
    16741 caaaaataat tcccgttgcc aaagcatcaa tactggtagc cactgccaaa ctcaatattt
    16801 tcagccaatg aaagggattg gattcgccgt tgttggcgag ttgatggttt ttcaaacctt
    16861 cataaagcat tcttccgcca atcagtgtca agaacccaaa agcaatccaa tgatcgcttt
    16921 tactaatgaa acttacaaaa cttttgccag cataataacc aattaaaggc atccctgcct
    16981 gaaataatcc aaacaaaaat gccatgaaca aggtcgaaga atagtagaat tttttcacac
    17041 tgattccctt gctgatggcc acagccaaac aatccatagc cagcccgatt cctataagaa
    17101 taatagtaac attgtccatg acaaaaacgg ttatagaaaa a
//
LOCUS       DAOSIB010000045        17131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_3538_length_17131_cov_11.637152, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000045 DAOSIB010000000
VERSION     DAOSIB010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17131
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3538_length_17131_cov_11.637152"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..2687)
                     /gene="topA"
                     /locus_tag="PLD44_05405"
     CDS             complement(249..2687)
                     /gene="topA"
                     /locus_tag="PLD44_05405"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOF98804.1"
                     /translation="MPKNLVIVESPAKAKTIEKFLGKEYHVVSSYGHIRDLSEKGIGI
                     DFEHNYQPQYKVLPDKKKLVSELKKATEQAELVWLASDEDREGEAIAWHLSEELKLPK
                     EKTRRIVFHEITKDAIVHAIEHPRDLDLNLVDAQQARRVLDRIVGFELSPLLWKKIRP
                     SLSAGRVQSVAVRLIVEREREINQFVPESSYRVTAIFSTTDKDGRSVELKAELQQRFS
                     TKEEALNFLNQCKQAIFRVENITTKPTKRSPAPPFTTSTLQQEAARKLGFSVSQTMLI
                     AQHLYESGKITYMRTDSVNLSDFALKAAEAEITNMLGKKYWKLRHYVTKTKGAQEAHE
                     AIRPTYMNVHEIDGSDQEKRLYDLIWKRTLASQMADAEFEKTTIQIGMQGQPYQFVAS
                     GEVILFDGFLHLYSESTDDESDSEKSEILPAINVGEKLLPKEILAQERFTQRPPRYTE
                     ASLVRKLEELGIGRPSTYAPTISTIQNRKYIEKGNRPGEKRTYNVLKLVDGKISDRSA
                     TEITGAEKAKLFPTDIGIVVNDFLIEYFPGILSYNFTANVEKEFDNVAAGKVGWTQVI
                     DKFYKKFHPTVEKTLTSTERKVGGRTLGTDPKTGKPVTVKIGRYGPYVQIGTAEDAEN
                     PVFASLRPGQSIDSITLEEALDLFKLPREVGEFEGKPLTVAIGRFGPYIRQGNSFYSI
                     PKGEDPLNVSLERAIEIIQHKREEDQKKTIKTLGENKEIQILNGRFGPYISFEKKNYK
                     IPKNVDPTGLTFEQCMEMIQEQAGTDKKGKKGNTRKTASTEKSAKNTKTSKTTKTTKT
                     SKTKKSTTAGKKKV"
     gene            2964..4865
                     /locus_tag="PLD44_05410"
     CDS             2964..4865
                     /locus_tag="PLD44_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOF98805.1"
                     /translation="MQKYLDILKQYWGYSSFRPLQDEIIGSIASGNDTLGLMPTGGGK
                     SLTFQVPAMAMDGVCVVVTPLIALMKDQVENLKKRGIAAAAIFSGMSYDEILMTLENA
                     SFGAYKFLYVSPERLATRIFIEKIKQTKVCLIAVDESHCISQWGYDFRPSYLKIAEIR
                     AFLPNAPVLALTATATPEVAEDIQKQLHFPAFNIFKKSFYRSNLAYVVRYTEDKNQYL
                     LKILNSVPGTSVVYVRNRKKTKETAEFLVKNGIVAEHYHAGLNNVKKDEIQTRWKNGK
                     TRVIVSTNAFGMGIDKADVRSVIHLDLPDSLEAYFQEAGRAGRDEKKAFAVLLYNNSD
                     AAKIKKRISDTFPPKEMIRKVYESLGNYYQVGIGSGLDNTFPFDLVDFCTKFHLPVLM
                     AFSSLKILQQAGYIEFTDEEESTSQVLFTVKKEELYQLKQTPKQEKLIHVLLRSYTGL
                     FTEPAFIHEEVVAQRLQSTPQEVYQQLVELSKEKVIRYIPRKKTAFLTFTQERVDAKQ
                     IYLSREAYEDRRERFVSRVQSVLNYAKEENVCRSQMLLSYFGEKNSQPCGKCDVCQKK
                     KETEISTAEFEAIRSSIVQVLSNENLPLDIVVQKIHFKEKKVLQVIRFMLDNNQIKEN
                     EKKQLILAK"
     gene            complement(4952..5767)
                     /locus_tag="PLD44_05415"
     CDS             complement(4952..5767)
                     /locus_tag="PLD44_05415"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOF98806.1"
                     /translation="MEKKVILITGASSGIGLSTAIQLMNKGYRVYAASRSGSENSTSK
                     NGGEIISTKMNVNSEEEIKKVLDEILEQNGHLDAVISNAGNGIAGSIEDTSIEEAKYQ
                     FETNFFGAVRVIQACLPIFRKQGYGKIIATSSVAAIVPIPFQAFYSASKAALLIFMQA
                     LDMETKPFGIQCSTILPGDTKTNFTSARKYTAASQSTESVYYEKMKKAVGKMEKDEQN
                     GMPADAIAKAIVKQIVHKSMKAIVVPGFQYQVICWLFNRLPVKFRLKVVSMLY"
     gene            5972..6967
                     /gene="trpS"
                     /locus_tag="PLD44_05420"
     CDS             5972..6967
                     /gene="trpS"
                     /locus_tag="PLD44_05420"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOF98807.1"
                     /translation="METVLSGIRPTGNLHLGNYFGALKNFVQMQNEYNCYFFIADYHS
                     LTTHPTPQNLTENVREILAEYLAAGLDPEKAVIYIQSDVPEVTELYLFLNMNAYVGEL
                     ERCTSFKDKVRQQPHNVNAGLLTYPTLMAADILLHRANKVPVGKDQEQHLEMTRTFAN
                     RFNRMYQVDYFPEPQAFNFGNELIKIPGLNGTGKMGKSEGEGNAIFLSDEPEAIRKKV
                     MKAVTDAGPTQPHQEKSQPIENLFQLMRAVSSAETLAYFEEQYDSCQIKYGEMKKQLA
                     EDMITFTAPFREKIKDLLSNKDYLSNVARMGKEKAQENARKTIRDVREIIGFKSI"
     gene            complement(7055..8650)
                     /gene="pckA"
                     /locus_tag="PLD44_05425"
     CDS             complement(7055..8650)
                     /gene="pckA"
                     /locus_tag="PLD44_05425"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOF98808.1"
                     /translation="MANLDLSKYGITGEYEIVHNPTYEQLFRAETDPKNEGFERGVVT
                     TTGAVAVDTGIFTGRSPKDKYFVKDEITEKAMWWDGVINRPINTEVWDYCRGLVTDRL
                     GSVKKLYVVDCFCGTNPDTRMKVRFVMEVAWQAHFVTNMFIRPSHYELANFGEPDFVV
                     LNGSKVTNPKWKEQGLNSEVFVLFNLSQRMAVIGGTWYGGEMKKGIFSLMNFYLPQKG
                     IASMHCSANVGEDGDVAIFFGLSGTGKTTLSADPKRYLIGDDEHGWDDHGVFNYEGGC
                     YAKVIDLSEENEPDIWRAIRRDALLENVTVLPDGTPDFSKAASKTENTRVSYPIYYIN
                     KIVLPSKAGHASKIIYLSADAYGVLPPVSILDEKSAQYHFLCGYTSKLAGTERGINEP
                     VPSFSPAFGEAFLTLHPTIYAKVLAEKMKKHNAKAYLVNTGWNGTGKRISIKDTRAII
                     DAIIDGSIEKAEKVHIPIMNLTIPKSLNNVNPAILDPRDTYANPAEWEQKAKSLAAQY
                     IKNFEQYLDNENAKELLSAGPQL"
     gene            8922..9929
                     /locus_tag="PLD44_05430"
     CDS             8922..9929
                     /locus_tag="PLD44_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOF98809.1"
                     /translation="MKVLFIGGTGIISSACTEIAIEKGMEVFHLNRGMTDKDHVPKEV
                     TTIHADIRNGNEAEAKLKGHHFDAVVDFIAFEPQHIEQDIRLFRDRTAQYVFISTASA
                     YQKPPEKLPITEKTPLWNPYWEYSQKKIFCEKLLKSEAKKSGFPFTIVRPSHTYNYKK
                     VPAIGGYTVLHRMLKGQPVVLHGDGTSVWTLTHHRDFAVGLIGLLGNRAAIHEDFHIT
                     SDEWLTWNGIYKILASELGVEPKTVHIPSEIIALYDKEMGANLLGDKSHSMIFDNSKI
                     KKIVPEFQAKTPFSKGATEIVQWFRENTLYTGPDAAVENWMNRLIEDWEGFKSFRGNV
                     K"
     gene            10074..12572
                     /locus_tag="PLD44_05435"
     CDS             10074..12572
                     /locus_tag="PLD44_05435"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOF98810.1"
                     /translation="MNKSLFQKLLPHFVALILFFCLSFWYFSPVLEGKQLMGHDTQSW
                     LYSAKETIDYNQTHDDKTLWTNSMFGGMPTYQISMTQLNNIIKYVDQLIKSLPSPVFF
                     LVLYLLCFYILLLAFGINPWLAIIGSFAFSFASYNFIILSVGHNSKAITIAYIAPLIG
                     SIYMAFRSKRMLGSLLTALFLSLAIYANHIQILYYTLLMVLVFGLVELIFSIKEKKAS
                     SYFKTLGLLIIAALLAAGMNATSLLTTYEYNQYTMRGKSTGLTLDEQNAQHGLNKDYI
                     TQWSYGIDETLTLLIPNFKGGASAGTLSSESKTAQRLSSYGVPNVERMMKEMQLPLYW
                     GNQPGTAGPVYVGAIVCFLFVLGIFLVDKRTKWWLLPLIVLSIMLSWGKNFMPLTDFF
                     IRYIPFYNNFRTVSMILVIASFGMALMAILALKEVFNPATDKAKLLRPVSISAAIVGG
                     ICLIFAVVPSLAGNFVSPADGQFTGDYAFLKETLPLDREALLRSDALRSLTFVILCAG
                     LIWLYAKNLVKKNLAYVLLGCLMLGDLVPVAKRYLNDDNFTRKRSFEKFIQPSVADKI
                     ILQDKSQFRVLDATVDIFNDASPSYFHKNIGGYHAAKLRRYQELINVHLTKELGQLFG
                     AFGRAKTIEEIVQVMDSLGVLNMLNMKYVIYNKEAPPLVNPYANGNAWLVTKVRIAEN
                     ADEEMRLLGEINTKTEVVVDKSFSALLPPAIIPDSSAHIQLVSYEPNHLIYNFSSKTD
                     QLAVFSEIYYDKGWNAYINGKKVPYIRANYLLRAMPLKAGSYQIEFKFEPDSYRIGNS
                     IALVSSILFVLWLVGYIYLRWKKPSMAKKIDQWE"
     gene            12635..13516
                     /locus_tag="PLD44_05440"
     CDS             12635..13516
                     /locus_tag="PLD44_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOF98811.1"
                     /translation="MGKNNNLHGKRSIKNIHFISTFSMSLVLFLVGLVILLLLTARNL
                     GINVRENINLSIILDDEIKNTEIQRIQKYLLSSPYAKSVRFISKEEALQEHIESLGED
                     PQKFLGYNPLLASFEVKLKAEYANNDSVEMIESKLKRFDHINRIAYQKDVVDLVNRNI
                     QKLSYILLGIALVLLLVSIALINNTIRLLIYSNRFLINTMKLVGATWWFIRKPYIKQG
                     MINGLVASILSLLYLTATIYYVKYEFGISGIVIPFGTALMVSSIILFLGVTLTAISSY
                     LAVGRYLRMGTNNMYFV"
     gene            13553..13978
                     /locus_tag="PLD44_05445"
     CDS             13553..13978
                     /locus_tag="PLD44_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HOF98812.1"
                     /translation="MKELTLITPTFLFSAVSLILLAYTNRFLSYAQLVRTLKDRYMED
                     HSAVTRAQILNLRKRLSLTRLMQLFGVASLLLCVATMFLIYVGFQTVSAYVFGIALVF
                     LIVSLSFSVWEIQISTGSLKIYLEEMENEEIREEEKLKT"
     gene            13989..15890
                     /locus_tag="PLD44_05450"
     CDS             13989..15890
                     /locus_tag="PLD44_05450"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOF98813.1"
                     /translation="MNYGFVRIAAAVPELKVSDVLFNTEKIIELIHVAEENRTQVVCF
                     PELCITGYTCADLFFQQQLQEEVLKALSEIQMATLSVSCAVIVGAPLRFRNRLFNTAV
                     VLQKGRIIGIVPKTFLPNHGEFYEKRWFVSGKNFHGQTIVCNEQEVPFGVDLIFFSPS
                     FSFGIEICEDLWAPLPPSTELCMAGAEIIFNPSASNELIGKHEYRLQLIEQQSARCHA
                     GYVYSSSGMYESTTDLVFSGSAVIAENGKILETSKRFSLDSQLIFNEIDVDILRYERM
                     HNSNFGEDALQQTYRKIEMQGQPLEQKEIKRTVSPYPFVPPVSKREESCSEIFSIQTS
                     GLAKRWLHTQAKSLIVGISGGLDSTLALLVCVKTADKLGFDRKTVIGVTMPGFGTTNR
                     TYGNALNLMKSLEITGLEISIKEACLQHFKDIGHDPDVHDVIFENTQARERTQILMDL
                     ANKYNGLVIGTGDLSELALGWATYNGDHMSMYAVNSGIPKTLVRYLVEYVAQSMEEKT
                     KTILEDILKTPVSPELLPADEYGEIHQKTEDLVGPYELHDFFLYYFVRFGFSPKKILF
                     LAQKAFGDKYSEAILRKWLKVFLRRFYSQQFKRSCMPDGPKVGSINLSPRGDWRMPSD
                     AGFHWDDII"
     gene            15932..16999
                     /locus_tag="PLD44_05455"
     CDS             15932..16999
                     /locus_tag="PLD44_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOF98814.1"
                     /translation="MLLFFERIILGNRLSDWGISLLIIVGAFLLNKGISLLNKNVIQK
                     ITAKSKNRLDDLLVKTLEAPILLGIALIAVWVAARRLDLGTQVDQGFYRAYQILTVLN
                     ITWFLVKLTGALINEYLLPHSKEEEDNKRLDESMVRILRRISVGIIWAIGIVMALNNA
                     GVNVGALLASLGIGGLAVALAAQDTLKNFLGGLTIFMDRPFRIGDRIVTGAYDGFVED
                     VGIRSTRIRNFDRRIISIPNYKIVDAAIENISIEPMRRVVLKIGLTYNTPPEKMKEAI
                     TILKNIPQKVDEVFPDEIYAYFTDFSDSALTITFIYFIKKEANVMETPSKVNMNILED
                     FNAAGLNFAFPTRTVYIENQN"
BASE COUNT         5017 a         3352 c         3448 g         5314 t
ORIGIN      
        1 ttcattgtga tgtatttttg tttcattgat aaaaatttca atgtttcttc aaaagaattt
       61 tattgatctg attaagtgtt taaaaggaca gctaatagta ttttgcaaaa aatatttcca
      121 ttattctcca aaaaaaaatg aattttttct catacagaca aaaaaatagc agaggaagaa
      181 aaaaaccggt tttttcttcc tctgctattt ttcctacttg cgcccagaaa tgtttcctga
      241 attttttatt aaactttttt cttgccagca gtcgtagatt tttttgtttt tgatgttttg
      301 gtcgttttag tcgttttgga ggtttttgta ttttttgcag acttttcggt cgaagctgtt
      361 ttcctcgtat ttcccttttt ccctttttta tctgttccgg cctgttcctg aatcatttcc
      421 atacattgtt caaaagttaa acctgtagga tcgacatttt taggaatttt gtagttcttt
      481 ttttcaaaag aaatgtaagg cccgaagcgt ccgtttaaaa tttgaatctc tttgttttcg
      541 cctaaagttt tgatagtttt cttctgatct tcctcccgtt tgtgttgaat aatttctatg
      601 gcgcgttcca gtgaaacatt caacggatct tcccctttcg gtatggaata aaacgaattt
      661 ccctgacgga tataaggacc aaaacgtcca atggcaacag tcagcggttt tccttcaaat
      721 tcccctactt cacgcgggag tttaaacaaa tcgagcgctt cttcgagtgt aatgctgtcg
      781 atggactgac ccggacgcag cgaagcaaaa acaggatttt cagcatcttc ggccgttcca
      841 atttgaacat aaggcccata tcttcctatt ttaaccgtaa ccggtttccc tgttttggga
      901 tcggtaccca acgtgcggcc gccaactttt cgctcggtgg aagttaaggt tttttcaacc
      961 gttggatgaa attttttgta aaacttgtcg ataacttgcg tccaacctac ttttcccgcc
     1021 gccacattat caaattcctt ttccacattg gccgtgaagt tataactcag aatcccggga
     1081 aaatattcaa ttaaaaaatc gttgaccact ataccgatat cagtcggaaa taattttgct
     1141 ttttcggcac cggtgatttc cgtagccgac cgatcggaaa tttttccgtc aacaagcttc
     1201 agcacattgt aagttcgttt ttcgcccgga cggtttcctt tttctatgta ttttcgattt
     1261 tgaatagtcg aaatagttgg agcatacgta gaaggtcgcc caatgcccaa ttcttcgagt
     1321 tttcgcacca aacttgcttc cgtatatcgg ggcggacgct gggtaaaacg ttcctgagcg
     1381 agaatttctt ttggcaacaa tttttcgccc acattaatgg ccggtaaaat ttccgatttt
     1441 tctgaatcgg actcatcatc tgtcgattcg ctgtaaagat gaagaaaacc atcaaataaa
     1501 atcacttcac ccgaagccac aaactggtaa ggttgacctt gcattccaat ttgaatggtg
     1561 gttttttcaa attccgcatc cgccatttgt gaagccagcg tacgtttcca tatcaggtca
     1621 tacaatcgct tttcctggtc tgaaccatca atttcgtgaa cattcatata agtaggacga
     1681 atcgcctcat gagcttcctg agcgcctttg gtttttgtaa cataatgtcg caatttccaa
     1741 tattttttcc ccaacatgtt ggtaatttcc gcttctgccg ccttcagggc aaaatccgac
     1801 aaattcactg aatcggttcg catataggtt atttttcccg attcgtaaag atgctgagct
     1861 atcaacatgg tttgagaaac cgaaaaaccg agtttccgtg cagcttcctg ctgaagagta
     1921 gaagtggtaa aaggcggtgc gggtgatctt ttggtcggtt tggtagtaat gttttccacc
     1981 cgaaaaattg cttgcttaca ttgatttaaa aaattcaatg cttcttcttt ggtggaaaaa
     2041 cgttgttgaa gttctgcctt caattctacg gaacgaccat ctttgtctgt tgtactgaaa
     2101 attgccgtta cgcgataaga cgattcggga acaaactgat tgatttctct ttcgcgttcc
     2161 acaatcagac ggacagctac cgactgaaca cgccctgccg aaagggaagg acgaattttt
     2221 ttccataaca gcggagaaag ttcaaaacca acaattcggt cgaggacacg acgcgcctgc
     2281 tgggcatcta ccaaattcaa atccaaatct cgcggatgtt caatggcgtg aacaatggca
     2341 tctttcgtta tttcatgaaa tacaattctg cgtgtctttt cttttggaag tttcagttct
     2401 tccgacaaat gccatgcaat agcttctcct tcgcggtctt catcagatgc cagccaaacc
     2461 aactctgcct gttcagtggc tttctttaat tccgaaacca gttttttctt gtcgggtaaa
     2521 actttgtatt gaggctgata attgtgttca aaatcaatac caatgccttt ttcggataaa
     2581 tcacgaatat gtccgtaact tgaaacaaca tgatattctt ttcccagaaa tttttcgata
     2641 gttttggctt tggccggaga ttctacaata acaaggtttt ttggcatact acaactttaa
     2701 attaatacgc ggtatttaaa attgcctgca aagtaacaaa atttgaaagg tatcagccaa
     2761 attttcagat tattattttt aaaatttata aaacgaaaac ataaaacgaa ctgaaatata
     2821 caaaatgttc acttcattga ataaaaggaa ataatctaat ttagtgaaca attttcacct
     2881 ttttttctct ttcctgattt acatataaaa atcggaactt tcggtaaaaa tcattatttt
     2941 tgtaataaag ttgcaattaa tttatgcaga aataccttga cattctcaag caatactggg
     3001 gatattcttc atttcgacca ttacaggatg aaattattgg tagcatagct tccggaaacg
     3061 atacgttggg acttatgcct accggcggag gaaaatcgct gacttttcag gtgcctgcca
     3121 tggcaatgga tggagtttgc gtggttgtta cacccttgat tgcactgatg aaagatcagg
     3181 tagaaaactt gaaaaaaaga ggaattgcgg ctgctgccat tttctctggc atgtcttacg
     3241 acgaaattct gatgacgctc gaaaatgctt catttggagc ctacaaattt ctttatgtat
     3301 cgcccgaaag acttgcaaca cgcattttta tcgaaaaaat aaaacagaca aaagtttgtc
     3361 tcattgctgt ggacgaatcg cactgtattt ctcaatgggg ttacgacttt cgaccttcct
     3421 acctgaaaat tgccgaaatc agagcgtttt tacccaatgc acccgtactt gcacttacgg
     3481 ctactgccac tcctgaagtc gccgaagaca tacagaaaca actacatttt ccggcattca
     3541 acattttcaa aaaaagtttt tatcgttcca atttggctta tgtggttcga tataccgaag
     3601 ataaaaatca gtatttgttg aaaattctca acagtgtacc gggcacttcg gtagtttatg
     3661 tgcgaaacag aaaaaaaaca aaagaaacag ccgagtttct tgtaaaaaac ggaattgtag
     3721 ccgaacatta tcatgcaggc ctcaacaacg tgaaaaagga cgaaattcaa acacgctgga
     3781 aaaacgggaa aacccgcgtc attgtttcca ccaacgcttt tggcatggga atcgacaaag
     3841 ccgatgtacg gtcggtgatt catttggatt tgcccgattc gctggaagca tattttcagg
     3901 aagccggaag agcaggccga gacgaaaaaa aagcttttgc cgttctgctt tacaacaaca
     3961 gcgatgcagc aaaaattaaa aaacgcattt cggatacttt tccgccaaaa gaaatgattc
     4021 gcaaagttta cgaatctttg ggcaattatt atcaggttgg tattggctca ggactggaca
     4081 atactttccc tttcgatctg gtcgattttt gcactaaatt tcacttgcct gtattaatgg
     4141 ctttcagcag cctgaaaatc cttcagcaag ccggttatat tgaatttacc gatgaagagg
     4201 aaagtacatc acaagtgctt ttcacggtta aaaaagagga attgtatcaa ctaaagcaaa
     4261 ctccgaagca ggaaaagctt atacatgttc ttttacgttc ttacacggga cttttcacgg
     4321 agccggcttt cattcatgaa gaagtcgttg cccaacggct tcaatctacc cctcaggaag
     4381 tgtatcaaca gttagtagaa ctgtcaaagg aaaaagttat ccgttacata ccaagaaaaa
     4441 aaacggcatt tcttactttt acacaggaaa gggtggacgc caaacagata tatttaagcc
     4501 gtgaagctta tgaagaccga cgagagcgat ttgtttcccg cgtgcagagt gtactgaact
     4561 atgccaaaga agaaaatgtg tgtcgaagtc aaatgttgct ttcctacttc ggcgaaaaaa
     4621 acagccagcc ttgtggcaaa tgcgacgtgt gtcaaaagaa gaaagaaacg gaaatttcta
     4681 ctgccgaatt tgaagccatt cgttcgtcaa ttgttcaagt actctcaaac gaaaatcttc
     4741 ccctcgatat agttgttcaa aaaattcatt ttaaagaaaa aaaggttttg caggttatcc
     4801 gctttatgct cgacaacaat caaataaagg aaaacgaaaa aaaacagctt attttggcga
     4861 aataaaatct ctcgccttgc agtttttagt atttcaagct tttttttaat cgtttaatgt
     4921 actttcagcg tatcagcaaa actttcagga actaatagag catacttacc actttcagcc
     4981 tgaatttgac gggcaatcgg ttgaaaagcc agcaaataac ctgatactga aatcccggaa
     5041 ctacgattgc tttcatgctt ttgtgtacaa tttgcttgac aatggctttt gcaatagcat
     5101 cggcgggcat cccgttttgt tcatcttttt ccatttttcc gacagctttt ttcatttttt
     5161 cgtaataaac cgattcggtc gattgagaag ctgcggtata ttttcttgcc gacgtgaaat
     5221 tggttttggt atcacccggt aaaatggtgc tgcactgaat gccaaaaggt ttggtttcca
     5281 tgtcaagcgc ctgcataaaa ataagcaaag ctgctttgga agcggaataa aatgcctgaa
     5341 aaggaatagg aacaatggca gctaccgaag atgtggcaat gatttttcca tagccctgtt
     5401 tcctgaaaat gggcaaacag gcctgaataa ctcttactgc tccaaaaaaa ttcgtttcaa
     5461 actgatattt ggcttcttca atggaagtgt cttcgataga accggctatg ccgttacctg
     5521 cattggaaat aacagcatcc aaatgcccat tttgttccaa aatctcgtcc aacacttttt
     5581 ttatttcttc ttcactattc acattcattt ttgtagaaat aatttctccg ccatttttgg
     5641 aggtgctatt ttcactacca ctacgggagg cagcataaac cctgtaaccc ttgttcatta
     5701 gttgaatggc tgttgataat ccaataccgc tgcttgctcc ggttattaat attactttct
     5761 tctccatatt acatctccat ttttaaaaat ttttctgcaa aaataatcaa atgcaaagaa
     5821 agatttatat ttccaacgtt ttttggaaaa attcttttgc ttctctccga atcaaattcc
     5881 tctgacttta attgagaagc tggatatttc tcctaaaatg attaattttg catttgaatt
     5941 taaaatacaa cactaaaatt ttttattttt gatggaaact gtacttagcg gcatacgtcc
     6001 gaccgggaac ttgcatttgg gaaactattt tggagcattg aagaattttg tacaaatgca
     6061 aaatgaatac aattgttatt tttttatagc cgattaccac tcactgacca cgcatcccac
     6121 accgcaaaat ctgacggaaa atgtgagaga aattttagcg gaatatttgg ctgcaggcct
     6181 tgatcccgaa aaagcagtta tttacataca gagtgatgtg cccgaagtta cggaactgta
     6241 tcttttcttg aacatgaatg cttacgtcgg tgaattggaa cgctgtactt ccttcaaaga
     6301 taaagttcgc cagcaacccc acaacgtaaa tgccggactg ttgacttatc cgacattgat
     6361 ggctgccgat attttgctgc atcgcgccaa caaggttccg gtaggcaaag accaggaaca
     6421 acatctcgaa atgacgcgga cttttgccaa tcgattcaac agaatgtatc aggtagatta
     6481 tttccctgaa ccgcaagctt ttaatttcgg aaacgaactg attaaaattc cgggactcaa
     6541 cggtacggga aaaatgggaa aatcggaagg agaaggcaat gccattttct tatcggacga
     6601 gccggaggca attcggaaaa aagtaatgaa agcggtaacc gatgcaggcc ccacacagcc
     6661 tcaccaggaa aaatctcaac ccatcgaaaa tctttttcaa ttgatgagag ccgtatccag
     6721 tgccgaaact ttggcttatt ttgaagaaca atacgattct tgccaaatca aatacgggga
     6781 aatgaaaaaa cagctggcag aagatatgat tacttttact gctccgtttc gtgaaaaaat
     6841 taaagacctt ctgagcaata aagattacct gtcgaatgtt gcccgcatgg gaaaagaaaa
     6901 agcacaggaa aatgcgcgga aaaccattcg cgatgttcgc gaaataatcg gtttcaaatc
     6961 aatataaata acttctttct ctacttttta gaagaaaaaa gggaactgct caaaaatgaa
     7021 cggctccctt tttatatttc aggtatgtaa cttattaaag ttgagggcct gcagataaaa
     7081 gttctttggc attttcgtta tcgagatact gttcaaagtt cttgatatat tgagcagcca
     7141 aagattttgc tttctgttcc cattctgccg gattagcata ggtgtcgcgc ggatcaagaa
     7201 tagcagggtt cacattgttc aaagattttg gaatagtcag attcataatt ggaatatgaa
     7261 ctttttctgc tttttcgatt gaaccgtcaa taatggcatc gataatggcc cgggtgtcct
     7321 taatggaaat acgtttgcca gtaccattcc aaccggtatt aaccaaataa gcctttgcat
     7381 tgtgcttttt cattttttcg gccaacactt tagcataaat ggtaggatgc aatgttaaaa
     7441 atgcttctcc aaatgcaggc gagaaagaag gaacgggttc gttgataccg cgttcggttc
     7501 cggccaattt tgaagtatat ccgcaaagga agtgatactg tgccgatttt tcatccaaaa
     7561 tagaaaccgg aggcaaaacg ccgtaagcat ctgccgacaa atagataatt ttgcttgcgt
     7621 gtccggcttt tgaaggaagc acaattttat tgatataata aattgggtaa gaaacacgtg
     7681 tattttccgt tttagaagct gcttttgaaa agtcgggtgt gccatcgggc aaaacggtaa
     7741 cattttccaa caaagcatcc cgacgaatag cacgccagat atccggttcg ttttcttcgc
     7801 ttaaatcgat aactttggca taacatccac cttcataatt gaatacgccg tgatcatccc
     7861 atccgtgttc gtcgtcacca attaaatagc gttttggatc ggcagaaaga gttgtttttc
     7921 ccgttcccga caaaccaaag aaaatggcta catctccgtc ttcgccaaca ttggcagagc
     7981 agtgcatgga agctatacct ttctgtggca gataaaaatt catcagtgag aaaattcctt
     8041 tcttcatttc accgccgtac catgttccac cgataacggc catacgctga gaaagattga
     8101 aaagaacaaa aacttcagag ttcaatccct gttccttcca tttaggattg gtaaccttag
     8161 aaccattgag cacaacaaaa tcgggttcgc caaaatttgc cagttcatag tgtgaaggac
     8221 ggataaacat gttggttaca aaatgtgcct gccaagccac ttccataaca aagcgcactt
     8281 tcattcgcgt atcgggattg gtaccgcaga aacaatctac cacataaagt tttttcacag
     8341 aacccagcct gtcagttacc aagcctctgc aatagtccca aacctccgtg ttgatgggtc
     8401 gattaataac cccgtcccac cacatggctt tttcggtaat ttcatctttt acaaaatatt
     8461 tatctttggg cgaacgtccg gtaaaaatac cggtatctac tgctactgca ccggttgtgg
     8521 taacaacacc gcgttcaaaa ccttcgtttt tagggtcggt ttctgctctg aacagttgtt
     8581 cataagtagg attgtgtacg atttcgtact cgccggtaat accgtactta cttaaatcta
     8641 aatttgccat tttttttaaa attaattgtt atgtttgttt ttatcttttt tctgaaaagc
     8701 cttgtttttc acgtcgcaaa attacaacta ttttttttca cgacaaagga attaaagaaa
     8761 aatttcttta attaattaca aaaaaaactg taacttcgtt gagttaaacg aaaaacttta
     8821 aagtttatga aacccacatg tattacatac acaaacacga atgaatataa tattcagaca
     8881 tgtgggttcc atcagaccat taaaaaataa attatattct gatgaaagtt ttatttattg
     8941 gcggaacagg cattatcagt tcggcatgta cggaaatagc cattgagaaa gggatggaag
     9001 tatttcattt gaatcgtgga atgaccgata aggaccatgt gccgaaagaa gttacaacca
     9061 ttcatgccga tattcgcaac ggcaatgaag cggaggctaa gctgaaagga catcattttg
     9121 atgccgttgt cgatttcatc gcttttgagc cgcaacatat cgagcaggat atccgactgt
     9181 ttcgagaccg taccgctcaa tatgtgttta tcagtactgc atcggcttat cagaagccac
     9241 ccgaaaaatt gccaattacc gaaaaaactc cactatggaa tccttattgg gaatactccc
     9301 aaaagaaaat tttctgtgaa aaactattga aaagtgaagc aaaaaaatcc ggctttcctt
     9361 ttaccatagt tcgtccttcg catacctaca actataaaaa agtgccggcc atagggggtt
     9421 acactgtatt acatcggatg ttgaagggtc aaccggtagt gttacatggt gatggaactt
     9481 cagtttggac tttgacccat catcgcgatt ttgcagtcgg attaatcgga ttgttgggaa
     9541 acagagcagc cattcacgaa gattttcata tcacttccga cgaatggttg acgtggaacg
     9601 ggatttataa aattctggca agcgaattgg gtgttgagcc aaagacggta catataccat
     9661 cggaaattat tgccttgtac gataaggaga tgggcgccaa tttattggga gacaaatcgc
     9721 acagcatgat tttcgacaat tccaaaataa aaaaaattgt gcccgaattt caagctaaaa
     9781 ctccatttag taaaggagct acggaaattg ttcaatggtt cagagaaaat acgctgtaca
     9841 caggacccga tgcagccgtt gagaattgga tgaatcgttt aatagaagat tgggaaggat
     9901 tcaagtcgtt tcgaggcaac gttaaatgat taaagtttcg taaaccataa ggcttttatt
     9961 cctaaatgca agttactgta attttaaggt atgagaaaga gcttttgaaa aaaaataatg
    10021 tatttttgta ctcaaatttc ctcatttagc tgaaaatata aaccatcatt cttatgaaca
    10081 aaagtttatt tcaaaaattg ctgcctcatt ttgtagcact tattttgttt ttttgtttgt
    10141 cgttttggta tttttctccc gttttggaag gcaaacaact gatgggacac gatacccaaa
    10201 gctggttgta ctcagccaaa gagaccattg attataacca gacacatgat gataaaacat
    10261 tatggactaa ctccatgttt ggtgggatgc cgacttatca aatttcaatg acgcaactta
    10321 acaatattat taaatatgtc gaccaactta tcaaatcctt gccttcaccg gtattttttc
    10381 tggttcttta tcttttgtgt ttttatattt tgcttttagc ttttggtatt aatccctggc
    10441 ttgccattat tggttcattt gccttttcct ttgcctctta caattttatt attctgtctg
    10501 tcggacataa ttccaaagcc attacgatag cttacattgc accgcttatc gggagcattt
    10561 acatggcttt ccgttccaag agaatgttgg gaagcctatt gacggcactt ttcctgagtt
    10621 tagccattta tgccaaccat atccaaattc tttattatac tttgcttatg gttttggttt
    10681 ttggattggt ggaactgatc ttcagtataa aggaaaagaa ggcaagttct tattttaaaa
    10741 cgttgggatt gttgattatt gctgctttgc tggcagcggg aatgaatgcc acgagtttac
    10801 ttactaccta cgaatacaat cagtacacca tgcgcggaaa atcgacagga cttacccttg
    10861 atgaacaaaa tgcgcagcac ggactgaaca aggattatat tacacaatgg agctacggaa
    10921 ttgatgaaac gctgacattg cttatcccga attttaaagg gggtgcaagt gccggcacac
    10981 tttcatccga aagcaaaaca gcccagcgac tgtcaagtta tggggttccc aatgtggaaa
    11041 gaatgatgaa ggagatgcaa ttacctctgt attggggtaa tcagccggga actgccggac
    11101 cggtgtatgt tggagccatc gtctgttttc tgtttgtgtt gggaattttt ttggtggaca
    11161 agcgtacaaa atggtggttg ttgccattaa ttgttctttc tattatgctt tcgtggggga
    11221 aaaatttcat gccccttacc gactttttca tccgatatat tcccttttac aataatttcc
    11281 gcacggtttc catgattttg gtcattgcca gttttggtat ggcattaatg gcaatactcg
    11341 ccttaaagga agtattcaat cccgcaacag acaaggcaaa acttttgcgt cctgtttcca
    11401 tcagcgccgc aatagttggt ggtatttgtc tgatttttgc tgttgttcct tctttggccg
    11461 ggaattttgt ttcgcctgcc gatgggcaat ttacgggtga ttatgctttt ttgaaagaaa
    11521 cacttcccct cgacagagaa gctttactcc gcagcgatgc tttgcgttca ttgacttttg
    11581 ttattctttg tgccggcttg atttggctgt atgccaaaaa tctggttaaa aagaatcttg
    11641 cctatgtttt gttgggatgc ttgatgctgg gagacctggt gccggttgcc aaacgatatt
    11701 tgaacgatga taattttacg cgaaaaagaa gctttgaaaa atttattcag ccatcggtag
    11761 ccgataaaat tattcttcag gacaagtcgc aattcagggt actcgatgcc acagtggata
    11821 ttttcaacga tgcttcacct tcatattttc acaaaaatat aggcggatac catgcggcaa
    11881 aattgcgtcg ttatcaggaa cttatcaacg ttcatctcac taaggagctc ggacaattgt
    11941 ttggagcgtt tggaagagcc aaaacaattg aagagattgt gcaggtaatg gattctttgg
    12001 gcgttttaaa tatgctgaac atgaaatatg taatttacaa caaggaagcg cctccattgg
    12061 tcaatcctta tgccaatggc aatgcatggt tggtgacaaa agttagaata gccgaaaatg
    12121 cagatgaaga aatgcgtttg ttgggtgaaa tcaataccaa aacagaagtg gtggtggaca
    12181 aatctttttc tgcgctattg cctcctgcca ttattcccga ttcgtcggct cacattcaac
    12241 tggtaagcta cgaacccaat cacctgattt acaatttcag ttccaaaacc gatcagctcg
    12301 ctgttttttc agaaatatat tacgataagg gatggaatgc ctatatcaac ggcaagaaag
    12361 ttccctatat tcgtgccaac tacttgctta gagcaatgcc actgaaagcg ggttcttatc
    12421 aaatagaatt caaatttgaa cccgattctt acagaattgg gaacagtata gcccttgttt
    12481 cttccatatt gtttgtgctt tggttggtgg gttatattta tttaagatgg aaaaaacctt
    12541 ctatggcaaa gaaaatcgac caatgggaat aatttctgga ataaggattt gtaaagggtt
    12601 caaagagata attatttgaa aaagaatatc ggaaatgggt aaaaacaata atcttcacgg
    12661 taaacgaagt ataaaaaaca tacattttat ttctaccttc agcatgtcgt tggtgctgtt
    12721 tttggttgga ttggtaattt tattgctttt gacggcaaga aatctgggca taaacgtaag
    12781 agagaacatc aatctttcca ttattttgga cgatgaaatt aaaaacacag agattcagag
    12841 aattcaaaaa tatcttcttt cttctcctta tgccaaatcg gtgaggttta tctccaaaga
    12901 ggaagctttg caggagcata tcgaaagttt gggcgaagat ccgcaaaagt ttttgggata
    12961 caaccctttg ctggcttctt tcgaggtgaa actgaaagcc gaatatgcca ataacgacag
    13021 cgttgaaatg attgaatcta aactaaaaag atttgaccat atcaatcgca tagcctatca
    13081 gaaagacgta gtcgatttgg taaatcgaaa tattcagaaa ttgagttaca ttctgctcgg
    13141 aattgctttg gtattgttgc ttgtttctat agcattgata aacaacacca ttcgactgct
    13201 gatttattcc aatcgttttc ttatcaacac catgaaattg gtgggtgcta cctggtggtt
    13261 tatccgcaaa ccttatatta agcagggaat gatcaatgga ctggtggctt ctatcctatc
    13321 tctgctttat ttgacggcta cgatttatta tgttaagtat gaatttggta tttcgggaat
    13381 agtaattccc tttggtacag ctttgatggt atcctctatt attttgtttt tgggtgttac
    13441 gcttactgct atttcatcct atttggcggt aggcagatat ttgcgtatgg gcaccaacaa
    13501 tatgtatttt gtataatttt tttcggtttt ttaatttgta actgtcttta ccatgaaaga
    13561 actcacctta attaccccga cttttctttt ttcggcagtt tcgcttattt tgctggctta
    13621 taccaatcgt tttctgtcgt atgcccagtt ggtgcgaaca cttaaagatc gttacatgga
    13681 agatcattcg gcagttacgc gggctcagat tttaaatctc cgtaaaagac tttcgcttac
    13741 gcgactgatg caattgtttg gcgtggctag tttattgctc tgtgtagcta ccatgtttct
    13801 tatttacgtc ggttttcaaa ccgtttccgc ctatgttttc ggtattgcac ttgtattcct
    13861 gattgtttct ctttcctttt ctgtttggga aattcagatt tctaccggtt cgctcaagat
    13921 ttatttggag gaaatggaaa acgaagaaat cagggaggaa gaaaaactta aaacttgaag
    13981 aaatggaaat aaattatggt tttgtccgca tagcagctgc ggttcccgaa ctcaaagttt
    14041 cggatgtttt gtttaacact gaaaaaataa tagaactgat tcatgttgcc gaagaaaacc
    14101 ggacccaagt ggtctgtttc cccgaattgt gtatcaccgg ttacacgtgc gccgatttat
    14161 tttttcagca acaattgcaa gaagaagtat tgaaagccct gtctgaaatt cagatggcca
    14221 ccttgtccgt ttcctgtgct gttatcgtgg gagcaccgct tcgttttcgt aataggcttt
    14281 tcaatacggc ggtcgtctta caaaaagggc ggattatcgg catcgtccct aaaacttttt
    14341 tgcccaatca tggcgaattt tacgaaaagc gatggtttgt ttccggaaaa aattttcatg
    14401 gacaaactat tgtgtgcaat gaacaggaag tgccttttgg cgtcgattta atattttttt
    14461 ctccttcatt ttcttttgga atcgaaattt gtgaagattt atgggcaccg cttcctccca
    14521 gtaccgaatt gtgcatggca ggggcagaaa ttatttttaa tccttcggcc agcaatgaat
    14581 tgattggaaa acacgaatat cgactgcaac ttattgaaca acagtcggct cgttgccatg
    14641 ccggatatgt ttattcttct tccggaatgt acgaatcgac taccgacttg gtcttttcag
    14701 gaagtgcagt cattgcagaa aacggaaaaa ttctcgaaac ttccaaaaga ttttcgctcg
    14761 attctcagct gattttcaat gaaatagatg tcgatatatt gcggtacgaa agaatgcaca
    14821 acagtaattt cggtgaagat gctttacagc agacttacag gaaaattgaa atgcaggggc
    14881 aacctctcga acagaaggaa attaaaagaa cggtttcacc ctacccgttt gttccacctg
    14941 tatcgaaaag agaagaaagc tgcagcgaga ttttttccat tcaaacgtcg gggttggcca
    15001 aacgctggct gcatacacaa gccaaatcgc tgatagttgg aatttccgga gggctggatt
    15061 ctacgcttgc tttgctggtg tgtgtaaaaa ctgccgacaa attgggtttt gacagaaaaa
    15121 ctgttattgg ggttaccatg cccggttttg gcacaaccaa ccgaacgtat ggcaatgcgt
    15181 tgaatttgat gaaatctttg gaaattaccg gtttggaaat ttccatcaag gaagcttgtc
    15241 tgcaacattt caaagacatt ggacacgacc cggatgttca cgacgtgatt tttgaaaata
    15301 cacaggcacg tgaacgaacg caaattttaa tggatttggc aaataaatac aacggattgg
    15361 tgattggaac cggcgacctt tcggaattgg cgctgggatg ggcaacatac aacggcgatc
    15421 acatgtcgat gtatgccgtt aacagcggta ttcccaaaac tttggttcgt tatctggtag
    15481 aatatgtggc tcaaagtatg gaagaaaaaa ccaaaacaat tttggaagat attttgaaaa
    15541 cgcctgttag tcctgaatta ttaccagctg atgagtatgg agaaattcat caaaagacgg
    15601 aagatttggt gggtccttac gagttgcacg atttctttct ttactatttt gttcgttttg
    15661 gcttcagtcc aaaaaaaatt ctttttttgg cgcaaaaagc atttggcgat aaatattcgg
    15721 aagcaatcct gcggaaatgg ctaaaagttt ttttacggcg tttttattcg caacaattca
    15781 agcgttcgtg tatgcccgat ggcccaaaag tcggctccat caatctttcg ccgcgtggcg
    15841 actggcggat gccgagcgat gccggttttc attgggacga tataatttga tttctttata
    15901 aacactgttt catttcataa atttaaaatt catgttatta ttttttgaaa gaataatttt
    15961 gggaaaccga ctgtccgatt ggggaatctc tctgttgata attgtgggtg catttttgtt
    16021 gaataaaggt atttcacttt tgaacaaaaa cgtaattcag aaaattacgg ccaaaagtaa
    16081 aaatcggctg gacgatttat tggtaaaaac actcgaagcg cctattctac ttggtattgc
    16141 tttgattgcc gtatgggtgg ctgctcgccg tctcgatttg ggtacacagg tcgaccaagg
    16201 cttttatcgc gcttatcaga tattgacggt tcttaatatc acatggtttt tggtaaaatt
    16261 aaccggtgcc ctgatcaatg aatatcttct gccgcactca aaggaagagg aagataacaa
    16321 aaggctcgat gaaagcatgg tcagaatttt acgacgcatt tcggtgggca ttatttgggc
    16381 tattggcatt gtgatggcat tgaacaatgc aggtgtcaat gtgggagctc tgctggccag
    16441 tttgggaatt ggaggtttgg cagtggcttt ggcggcacag gacacgttaa aaaattttct
    16501 tggcggactc accattttta tggatcggcc ctttcgcata ggcgatcgga ttgttaccgg
    16561 agcctatgac ggttttgttg aagatgttgg cattcgcagc accagaatac gaaatttcga
    16621 cagaagaatc atatccattc ccaattacaa aattgtagat gccgccatag aaaatatttc
    16681 cattgaaccc atgcggcgcg tagtgctgaa aatcggcctt acttacaaca ctcctcctga
    16741 aaagatgaaa gaagccatta ctattttgaa aaatattccg caaaaagttg atgaggtgtt
    16801 tccagatgaa atttatgctt atttcaccga cttttccgat tcggcactga caataacatt
    16861 tatttacttt attaaaaaag aggcaaatgt gatggaaaca ccctcaaagg taaatatgaa
    16921 tattttggaa gatttcaatg cagcaggtct aaactttgct ttccctacgc gtactgtgta
    16981 cattgaaaat caaaactgac aatcggaatc caaaagaccg gtaattttgt ggtagcttaa
    17041 tggtgaactc gtttaagata caaataagaa catgaaaaaa acggaaacgg cacagaagca
    17101 tctttagaaa aacaatcgct cggcaagctc g
//
LOCUS       DAOSIB010000046        16473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_3689_length_16473_cov_13.046717, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000046 DAOSIB010000000
VERSION     DAOSIB010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16473
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3689_length_16473_cov_13.046717"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PLD44_05460"
     CDS             <1..381
                     /locus_tag="PLD44_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOF98815.1"
                     /translation="RITSPINGTVLVKYAEKGELAGAGKPLFKVADLQKMILRAYVTA
                     DQVTQLKIGQKAKVFVDFGKDDQKEYEGTVSWISGESEFTPKTIQTRDERANLVYAVK
                     INVKSDGLLKIGQYADVKFGAENQ"
     gene            421..1365
                     /locus_tag="PLD44_05465"
     CDS             421..1365
                     /locus_tag="PLD44_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF98816.1"
                     /translation="MTVNKGEFAVCCENITKTYPPDIQALKNINLKISPHQIFGIIGP
                     DGAGKTTLFRILTTLILADEGKATVLGYDVVNEYQEIRKYVGYMPGRFSLYQDLTVEE
                     NLKFFASVFGTTVEENYDLIKNIYRQIEPFKNRRAGQLSGGMKQKLALSCALIHKPKL
                     LILDEPTTGVDPVSRREFWEMLQNLKNEGITILVSTAYMDEAGLCDTIALILNGEFLK
                     IDTPKNIVQQFEPEIFSVQSNQRSKLLTDLRRLPEVSSCYAFGDSIHVTVENKKISEE
                     ELQNFLLKNGHEQIEIAKIEPTVEDCFMELSKQQEENN"
     gene            1368..2108
                     /locus_tag="PLD44_05470"
     CDS             1368..2108
                     /locus_tag="PLD44_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF98817.1"
                     /translation="MEKVIEVQNLTKKFGDFTAVDHISFEVYKGEIFGFLGANGAGKT
                     TAMRMLCGLLPPTSGKGLVAGFDIHTQSEEVKRNIGYMSQKFALYEDLKVWENIRLYG
                     GIYGVPEDEIIKKTDELLKRLGFEKERNSFVKSLPLGWKQKLAFSVAIFHEPKIVFLD
                     EPTGGVDPVTRRQFWEMIYEAADRGITVFVTTHYMDEAEYCDRVSIMVDGRIDALDSP
                     KNLKNQFKANNMDGVFQQLARRASRKAD"
     gene            2110..3216
                     /locus_tag="PLD44_05475"
     CDS             2110..3216
                     /locus_tag="PLD44_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF98818.1"
                     /translation="MKQFMSFVRKEFYHIFRDKTTMAILLILPILMLVLFGYSISTEV
                     KNSKIAILDSSNDVATRAIIEKFRNSEYFSVKKMVNNPSEIQDLFKKDRLNMVLVFGD
                     NFYENLAHTGNAQVQLIVDGSDPNSAKTIVTYATSIIGAYQLELSKIDKIPFQINPQI
                     KLLYNPQMKGAYNFVPGVMGMILMLICAMMTSISIAREKELGTMEVLLVSPMKPIYII
                     LSKTIPYFVLSLVNLATILLLSVFLLKVPIAGSLFWLIAFSLVYIFVNLSLGLLISSI
                     VDSQLVALLISGMVLMLPVIMLSGMMFPVENMPVFFQWISQIIPAKWYIIGVKKIMIK
                     GLGLSSIIPEMSVLLGMGIVLVIASLKNFKYRLE"
     gene            3234..4346
                     /locus_tag="PLD44_05480"
     CDS             3234..4346
                     /locus_tag="PLD44_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF98819.1"
                     /translation="MLKYLLEKEFKQIMRNKFLPRMVIILPFVALALFPLVANFDVKN
                     INLAVVDNNHSQYSERLIQKVISSGHFILTNVSHNYSDALKSVEQNKSDVILEIPAQF
                     EKDLVNNLTASVLISANTVNGMKGGLSSAYLVGIITDFSSEIREEWIQSGTKLKVPAC
                     EIIPQYRFNPHLEYKYNMIPAIMMMMLAMITGFLPALNIVGEKEKGTIEQMNVTPVNK
                     FTLILSKLIPYWVIGFIVLTICFFVAWLFYGMISKGGYLQIYLFAAVFVLAFSGLGLV
                     ISNYATTIQQGMFMMFFFVITFIFLSGLYTPISSMPEWTQTMSFFSPLRYMIEALRSI
                     YLKGSSFMDLWHQFMALVGFAIFFNFWAINSYRKKA"
     gene            4398..5309
                     /locus_tag="PLD44_05485"
     CDS             4398..5309
                     /locus_tag="PLD44_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HOF98820.1"
                     /translation="MGNLNFKVQSLFCEQVSEWELAKKNYEDLKKVEIKVFSFGDFEI
                     VVQFNPARIVSSGAKVDEKTIKERKCFLCRQNLPPQQKCVDAEDYTVLVNPYPIFPEH
                     FTIPRKEHIPQQIKPFFPDMLELAKSLDEFVVFYNGPKCGASAPDHMHFQAGTKNFLP
                     LVNDYRRLKATHAEILEKENHYSLSFFKNYLRTVYCMESDDSQVLSFQFNRLYEDFQK
                     KMNCQANEEPMMNVVCLFENQKWIVFVFPRKAFRPWQYSAEESRQLMVSPATVEMSGI
                     FITPVEEHFRRITREDVVDILEQVSLK"
     gene            complement(5437..8049)
                     /gene="mutS"
                     /locus_tag="PLD44_05490"
     CDS             complement(5437..8049)
                     /gene="mutS"
                     /locus_tag="PLD44_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOF98821.1"
                     /translation="MSEPIVETPLMKQYFEMKSKHPDAILLFRCGDFYETFSTDAIEA
                     SEILGITLTRRANGSAKSVELAGFPHHALDTYLPKLVRAGKRVAICDQLEDPKLAKKI
                     VKRGITELVTPGVTYNDTILNHKENNFLASVYLNKNQAGVAFLDVSTGEFLLAEGTNE
                     YVDKLLNSFSPKEIIFDRTKKKEFENLFGTKFYSYPLEDWVFTLDAAKDRLLKQFETK
                     SLKGFGVEGFANGVVAAGAILYYLDLTHHQQTGHITQLSRIEEDRFVWLDRFTVRNLE
                     LFEAINEGGKTLLEVLDKTISPMGSRLLKRWMAFPLKNVKSINERLDVVEYFFKHPEL
                     KEALAQNIALIGDLERLVSKIAVGRINPREVVQLKTALKAIEPIKQLCTHTENITLRT
                     IADQLNACSIISEKIEREIQNDPPILINRGNIIQKGVNADLDELRELAFSGKDFLQKI
                     QERESQATGIPSLKVGFNNVFGYYLEVRNTYKEKVPEAWIRKQTLVNAERYITEELKE
                     YEEKILGAEEKIIDIETRLFNELVLSLSEYIPALQLDSNLIAQLDGLLSFAKVSEENR
                     YVRPEINDTEVLEIHQGRHPVIEKQLPLGESYVANDVYLDSSKQQIMIITGPNMSGKS
                     ALLRQTALITLMAQMGCFVPVESAKIGVVDKIFTRVGASDNISLGESTFMVEMSEAAS
                     ILNNLSSRSLVLFDELGRGTSTYDGISIAWAIVEYIHEHPAHKAKTLFATHYHELNEM
                     EKSFSRVKNYNVSVKEVDNKVIFLRKLVRGGSEHSFGIHVAKMAGMPQSIVKRAEQIL
                     KQLETDNRQSDISKPVDKIAQHREGYQLTFFQLDDPVLEQIRDEIKNLDINNLTPIEA
                     LNKLNEIKRIIMGK"
     assembly_gap    8063..8162
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8221..9351)
                     /locus_tag="PLD44_05495"
     CDS             complement(8221..9351)
                     /locus_tag="PLD44_05495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOF98822.1"
                     /translation="MKIIIVGTAYPYRGGLASFNERLAREYAREGHEVELITFTLQYP
                     NFLFPGKSQYSTEKPPEDLKIIRRINSINPFNWIKIGKEIKKKAPDLVIFCYWTSFIA
                     PCFATIARYARSPQTKMLGLVHNMIPHEATPLDKWLPGYFVKNMDGFLAMANSVKEDI
                     QHFDKKNKPKIVVPHPVYDHYGAIVSKSEAALKLGLPPKKRYVLFFGFIRHYKGLDLL
                     LEAFSDKRLKDMEVQLVVAGEFYENPEPYLKQIAQLGLANRVELRTNFIPENEVKYYF
                     SLADLVAQPYRSATQSGVSQIAYHFEKPMLVTNVGGLAEIIPNGKVGYVVNGQPKEIA
                     DAIVDFFQNNREEIFLPNIKTEKMKYGWDKMTAAINKLFFKL"
     gene            9538..9978
                     /locus_tag="PLD44_05500"
     CDS             9538..9978
                     /locus_tag="PLD44_05500"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006847857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOF98823.1"
                     /translation="MEENQDIKYMKQALAEARRAAEKDEVPIGAVIVCQGSIIARAHN
                     LTETLNDVTAHAEMQAITSAANYLGGKYLSDCTLYVTIEPCVMCAGALGWAQIKKIVY
                     GASDEKRGYSKFAPDALHPKTEVVSGVLAEECATLMQNFFKRKR"
     gene            complement(10055..10351)
                     /gene="trxA"
                     /locus_tag="PLD44_05505"
     CDS             complement(10055..10351)
                     /gene="trxA"
                     /locus_tag="PLD44_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOF98824.1"
                     /translation="METFQDIINGDKPVLVDFFATWCGPCKAMAPVIESLGKEVQGQA
                     RVLKVDVDKNQALAAKYGIQAVPTFMLFKKGNVIWKHSGAIDKNTLMQQIKSAV"
     gene            complement(10416..10856)
                     /locus_tag="PLD44_05510"
     CDS             complement(10416..10856)
                     /locus_tag="PLD44_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOF98825.1"
                     /translation="MATNRLTEQLPSNFDRYFDNEWEDYNWTDRELAANHTTLPAVNI
                     KENGEAFEIDVAAPGYEKSDIKIEMNEDLLTVSSNKKLDEEKTEGEYTKREFSYQSFL
                     RTFTLPETVDAEKISAVYENGILKITIPKKEEAKVKPARTIDIQ"
     gene            11353..12738
                     /locus_tag="PLD44_05515"
     CDS             11353..12738
                     /locus_tag="PLD44_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOF98826.1"
                     /translation="MKKFIVGFFILSIFISSYGQPNDYRRWSITLDAGINNFDGDNNQ
                     NYNQIIPNSAAKASFGFSTELTLSPFWGIGTEFFYFPVSAEDAAKKFNADVFHFTPYL
                     SLNLLNLCNENNHSKWNIYATVGAGVAYYNSTLYKNGIEFDRIRDGWAAVFPVGGLIE
                     YNISSLFAIGAKVQYRLQNKDNFEGTNLKEEAGGYNFKGVTNDNIAMAALSLRWKIGA
                     KSKNHIRNNETRITLGDVFTLANRANIKTDSLAQELDKLKLSLQPGPDDDGDGVPNSR
                     DLEPNTPKGLYIDYNGRGIPEIKVIPETKEIHPEYLQYISASTKDEDKDGVPDNRDKE
                     PNSPKNLCVDFWGRSIAQDKPCAFASVYFDFDKTNLDEEALIEIEKLAEKLNTDPTLL
                     VEIRGYADNVGSNKYNKRLSQERSNRVKAELVFTHNINPQRIISNGKGKIPQPPRAYR
                     MNRRCDFILSK"
     gene            12956..13813
                     /locus_tag="PLD44_05520"
     CDS             12956..13813
                     /locus_tag="PLD44_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013264050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HOF98827.1"
                     /translation="MSNNLLVGKRGIIFGALNDMSIAWKVAERVVEEGATVTLSNTPI
                     AIRMGTINELAEKINAKIIPADATNLDDLEQVFSQSMEILGGKIDFILHSIGMSPNVR
                     KKRPYDDLDYDLMHTTLDISAISFHKMLQTAKKMNAINEWGSVVALSYIASHRTMYGY
                     NDMADAKAMLESIARNFGYIYGREHNVRINTISQSPTPTTAGSGVKGFDSLMDFSERM
                     SPLGNASADDCANYCVVMFSDLTRKVTMQNLFHDGGFSSMGMSYRALQQYNKSFEGSQ
                     THDGKIIYG"
     gene            complement(13897..16056)
                     /locus_tag="PLD44_05525"
     CDS             complement(13897..16056)
                     /locus_tag="PLD44_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOF98828.1"
                     /translation="MKVYQPNEIKNISLLGSSGSGKTTLVEAMLFEGGIIKRRGTIAN
                     QNTVCDYFPVEKEYGYSVFPTLAQIEWRQHKLNIIDCPGSDDFIGAVVTSLNVTDMGL
                     IVVGAQYGVEVGTNNHFSYAEKYNKPVMFVVNQLDHDKADYEHTIEQLKENFGNKVIQ
                     LQYPLQTGSGFNSLVDVLQMKMLRFKTEGGAPEITEIPDSEKAKAQELHNSLIESAAE
                     FDESLMEKFFEEGILSDEEIYKGLYKGVLERGIFPVICVSAANDAGVSTLMDYLVRIA
                     PSVSDSKRPLTKDGKSVDPNPDGPTSIYVFKTNVEPHIGEISLFKVMSGKLKEGDDLY
                     NVNRQVKERISQIFCVAGQIRNKVEEISAGDFGATVKLKDTRTGNTLNGKGCDYDFVG
                     VEYPEPKYRRAIKAKSEADEEKLNEILTRMHEEDPTWIVEQSKELKQTILHGQGEFHL
                     RTLKWRVENNDKIEIEFKEPKIPYRETITKKARADYRHKKQSGGAGQFGEVHLIVEPY
                     FEDVPIQDVYRFNGQEYKISVRDVQEIDLEWGGKLVFVNSIVGGAIDARFLPAILKGV
                     MDRMEQGPLTGSYARDVRVIVYDGKMHPVDSNEISFRLAGRNAFAQAFKDAGPKLLEP
                     VYDVVVMTPSDRLGDVMSDLQSRRAVIMGMNSEKGFEKLQAKIPLKELSNYSTTLSSL
                     TGGRASFVMKFASYELVPIEIQEKLLKEYEASKKEED"
BASE COUNT         4883 a         3101 c         3210 g         5179 t
ORIGIN      
        1 agaattacca gtccgattaa tggaaccgtg ctagtgaaat atgccgaaaa aggggaattg
       61 gctggtgccg gcaaaccgct tttcaaagtt gccgacctgc aaaaaatgat tcttcgggct
      121 tatgttacag ccgatcaggt tacacaactg aaaattggcc aaaaagcaaa agtatttgtc
      181 gattttggca aagatgatca aaaagaatat gaaggaacag tcagttggat ttccggcgaa
      241 tcagaattta cgcccaaaac cattcaaacg cgcgacgaaa gagccaattt ggtttatgcc
      301 gtaaaaatta acgtaaaaag cgatggtttg ctgaaaattg ggcagtatgc tgatgttaaa
      361 tttggtgccg aaaatcaatg atcaataatt tttgatattt tccttaatag aaaatcgaga
      421 atgacagtaa ataaaggaga atttgcggtt tgttgtgaga acattacaaa aacttatcca
      481 cccgatattc aagcgctaaa aaatataaat ttaaaaatat ctcctcatca gatatttggg
      541 attattggtc ctgatggagc gggaaaaacc acattgtttc gtattctcac aacgctaatt
      601 ttggctgacg aaggaaaagc gactgtgctt ggttatgatg tggtgaatga atatcaggaa
      661 atcagaaaat atgttggtta catgcctggt cgattttcgc tttatcagga tttgacggta
      721 gaagaaaatt taaaattttt tgcttccgtt ttcggaacca cagtagaaga aaattacgat
      781 ttaattaaaa atatttatcg gcaaatcgaa ccttttaaaa accgaagagc cggccaactt
      841 tccggtggaa tgaagcaaaa attggcgttg agttgtgcgc ttattcacaa acccaagctt
      901 ctgattttgg atgaaccaac aaccggcgtc gatccggttt cgcgaaggga attctgggaa
      961 atgcttcaaa atctcaaaaa tgaaggaata actattttgg tttctacagc ttatatggac
     1021 gaagccggac tttgcgatac cattgctttg atattgaacg gagagttttt aaaaattgac
     1081 acccccaaaa atattgtgca gcaattcgaa ccggaaatat tttcggttca gagcaatcag
     1141 cgctcaaaac tgttgaccga tttgcgtcgt ttgccggagg tttcgagctg ttatgctttc
     1201 ggtgacagta ttcacgttac ggtagaaaac aaaaaaattt cagaagagga attgcagaat
     1261 tttcttttaa aaaacggaca tgaacaaatc gaaattgcca agatagaacc aactgttgaa
     1321 gattgtttta tggagctttc caaacaacag gaagaaaata attgagaatg gaaaaagtta
     1381 ttgaagtaca aaatttgacc aaaaaatttg gtgattttac ggctgttgat catatttcct
     1441 ttgaggtgta caagggagaa atttttggtt tcctcggcgc caacggagct ggaaaaacta
     1501 ctgccatgcg tatgctgtgc ggacttttgc cacccacttc gggaaaagga cttgttgccg
     1561 gtttcgatat tcatacccaa tcggaagaag tgaaaagaaa catcggctac atgagccaaa
     1621 aatttgcatt gtacgaggat ttgaaggttt gggaaaatat ccgtttgtat ggtggaattt
     1681 atggcgtgcc tgaagatgaa attataaaaa aaacagatga gttattaaaa cgacttggat
     1741 ttgaaaaaga aagaaattcg tttgtgaaat cgcttccttt gggatggaag cagaaacttg
     1801 ccttttcggt tgccattttt catgagccta agatagtttt tcttgatgag ccaacaggcg
     1861 gagtggatcc cgtaacgcgt cgtcagtttt gggaaatgat ttacgaagcc gccgaccgcg
     1921 gaatcaccgt ttttgttacc acgcattata tggacgaagc agaatattgc gatcgggttt
     1981 ccattatggt tgatggacga attgacgctc tcgattctcc aaaaaatcta aaaaaccagt
     2041 ttaaagccaa caatatggat ggagtttttc aacaacttgc gcgacgcgct tcaagaaagg
     2101 cagattagta tgaaacaatt catgtccttc gttcgaaagg aattttacca tattttcagg
     2161 gataaaacaa caatggctat tttgttgatt ttacccattc tgatgcttgt gttgtttgga
     2221 tattcaatca gcaccgaagt caagaattct aaaattgcca tactagattc ttcaaatgat
     2281 gtggcaacac gcgctattat tgaaaaattc aggaacagcg agtatttttc agtcaagaaa
     2341 atggtaaata atccgtccga aattcaggat ttattcaaaa aagacagact gaatatggta
     2401 ctcgttttcg gtgataactt ttatgaaaat ctggcacata cgggcaacgc acaagtacaa
     2461 ctaattgttg atggcagtga tcctaattcg gccaaaacaa ttgtgactta tgcaaccagt
     2521 attattggag cttaccaact ggaactatct aaaattgata agattccgtt tcaaataaat
     2581 ccacaaataa aattgctcta caacccacaa atgaaaggtg cttacaattt tgttccgggt
     2641 gtaatgggta tgatacttat gctgatttgt gccatgatga cttccatctc cattgcccgc
     2701 gaaaaagaac ttggcacaat ggaagttttg cttgtatcac caatgaaacc tatctatatc
     2761 atactttcca aaaccattcc ctatttcgtt ttgtcgctgg taaatttagc taccatttta
     2821 ctcttatccg tcttcctgct caaggttcca attgcaggga gcttgttctg gttgattgca
     2881 ttctcgctgg tctatatttt tgtaaatcta tcgttggggt tgttgatttc ttcgatagta
     2941 gactcgcaac tggtggctct gcttatctcg ggaatggtgc ttatgttacc ggtcattatg
     3001 ctatcaggaa tgatgtttcc ggtcgaaaat atgccggttt tctttcaatg gatttcgcaa
     3061 attatccccg cgaaatggta tattataggg gtcaaaaaaa tcatgattaa aggacttggt
     3121 ttatcttcca tcattccgga aatgagtgtt ttattaggaa tgggaatcgt gttggtgata
     3181 gcaagtctga aaaatttcaa gtataggttg gaataataac aggagacaaa attatgctga
     3241 aatatttatt agaaaaagaa ttcaagcaaa ttatgcgcaa caagtttttg ccccgtatgg
     3301 tcatcatctt gccgtttgtt gcactcgcgc tattcccatt ggtagccaac ttcgatgtta
     3361 aaaacattaa tcttgctgtg gttgacaaca accatagtca gtactccgaa cgtctgattc
     3421 agaaggtcat ttcgtcgggg cattttatcc tgacaaatgt ttcgcataat tactcagatg
     3481 cactgaaaag tgtagaacag aacaaatctg atgttatact cgaaataccg gctcaattcg
     3541 aaaaagactt agtaaataac cttactgcca gtgtgctgat ttcagcaaac accgtaaacg
     3601 gaatgaaagg tggactaagc agtgcctatt tagtcggaat aataaccgat ttcagcagtg
     3661 aaatcagaga agaatggatt cagagtggta caaaactaaa agttccagcc tgcgaaatta
     3721 ttccgcaata ccgatttaat cctcatctgg agtataagta caacatgatt ccggccatta
     3781 tgatgatgat gctggccatg ataaccggat ttttaccggc attgaatatt gtaggggaaa
     3841 aagaaaaagg aactattgag cagatgaatg taactccggt aaataagttc acgctcattc
     3901 tttcaaaact tattccgtat tgggtcattg gttttatcgt tcttacaata tgtttttttg
     3961 tggcatggct gttttacgga atgatttcaa aaggaggtta tctgcaaatt tatcttttcg
     4021 cagcggtttt tgtattggct ttctcaggtc tggggttggt tatctccaac tatgctacca
     4081 ctattcagca gggaatgttt atgatgttct tttttgtcat cacatttata tttctgagtg
     4141 ggctttacac gcctatttcc agtatgccgg agtggactca gacgatgagc tttttcagtc
     4201 cgctccgtta catgattgaa gctctgcgtt ccatttatct caaaggcagc agttttatgg
     4261 atttgtggca tcaatttatg gctttggtcg gttttgctat ttttttcaat ttttgggcta
     4321 tcaatagtta cagaaaaaaa gcttaaggtc caatttcaat ttttgaggaa ataaattttt
     4381 agacagaata atttttgatg ggaaatttga attttaaggt gcagagcttg ttttgtgaac
     4441 aagtttccga atgggagttg gccaaaaaga attacgaaga tttgaaaaaa gttgaaatta
     4501 aggttttttc ttttggcgat tttgaaattg tagtgcagtt taacccggca cgcattgttt
     4561 cgtcgggtgc aaaagtagat gaaaagacca taaaggaaag gaaatgtttt ttgtgtcgtc
     4621 agaatctgcc accacaacaa aaatgtgtcg atgcagagga ttatactgtt ttggtaaacc
     4681 cctaccctat ttttcccgag cattttacca ttccccgcaa agaacatatt ccgcagcaaa
     4741 taaaaccttt tttccccgat atgctcgaat tggcaaagtc gctggacgag tttgttgttt
     4801 tttacaatgg cccaaaatgt ggcgcttctg ctcccgatca catgcatttt caggccggga
     4861 caaaaaattt tttgcctctg gtcaatgatt atcgccgatt aaaagccacg catgccgaaa
     4921 tattggagaa agaaaaccat tacagtttat ctttttttaa aaattattta cgcacggttt
     4981 attgtatgga gtcggatgat agccaagttt tgtccttcca attcaatcgg ctttatgaag
     5041 attttcagaa aaaaatgaat tgtcaggcaa acgaagaacc catgatgaat gtagtttgcc
     5101 tgtttgaaaa tcagaagtgg atcgtttttg tttttccgcg aaaagcattc cgtccgtggc
     5161 aatattcggc tgaagaaagc cggcaattaa tggtaagtcc ggctaccgtg gagatgagcg
     5221 gtatctttat tacacctgtt gaagaacatt ttagaagaat tactcgtgaa gacgttgtag
     5281 atatattgga acaggttagt ttaaaatagc aaataaaaac acttgtttta aaaactcata
     5341 aatttagaat tataagtcaa taatcaagat gaaatttttt aaaaaaccaa ttatgattat
     5401 ctttaaaatt catcgaaggt tatgaacaga taaattttat tttcccataa taattctttt
     5461 gatttcgttc agtttattaa gggcttcgat aggagtcaag ttgtttatgt ccaaattctt
     5521 gatttcatct ctaatttgtt cgagtacagg gtcatcgagt tggaaaaaag tgagttgata
     5581 accttccctg tgctgggcaa ttttatctac cggtttggaa atatcggatt gtcggttatc
     5641 cgtttcgagc tgtttaagaa tttgttcagc gcgtttaacg atgctttgcg gcataccggc
     5701 cattttagcc acatgaatac cgaaactgtg ttcacttcca cctcgcacca gttttcggag
     5761 gaaaatcacc ttgttgtcca cttcttttac ggaaacattg tagtttttca cccgcgaaaa
     5821 ggatttttcc atttcattca gctcgtgata gtgggtagca aaaagcgttt ttgctttgtg
     5881 agcaggatgt tcgtgaatat attcaactat tgcccaagca atggaaatgc catcgtaagt
     5941 gcttgttcct cttcccagct catcaaaaag caccaagctg cgagaggaaa gattgttgag
     6001 aatactcgcg gcttcgctca tttcgaccat aaaagtcgat tcgcccaaag aaatgttgtc
     6061 gctggctcca acacgagtaa aaatcttgtc cacaacgcca atcttggcgc tttctaccgg
     6121 cacgaagcat cccatttgcg ccataagagt aatcaaagcc gtttgtcgaa gtaaagccga
     6181 tttaccggac atgttggggc cggtaataat catgatttgc tgtttcgagc tgtcgagata
     6241 cacatcattt gccacatagc tttcgcccaa aggaagttgt ttctcgataa ccggatgtct
     6301 gccctgatga atttccagca cttcagtatc gttgatttcg gggcgaacat aacggttttc
     6361 ttccgaaact ttcgcaaagg aaagcagacc atccagctgg gctatcaagt tcgaatcgag
     6421 ttgaagggca ggaatgtatt cggaaagact taacaccagt tcgttgaaca aacgggtttc
     6481 aatatcaata attttttctt ctgccccgag tattttttct tcatattctt ttaattcctc
     6541 ggtgatatag cgttcggcat ttaccagtgt ctgtttgcga atccatgctt cgggaacttt
     6601 ttccttgtag gtatttcgta cttcgagata atagccaaaa acattgttga atccaacttt
     6661 taaacttgga attcctgtgg cttggctttc acgttcctga atcttttgca ggaaatcttt
     6721 tcccgaaaaa gccagttctc tcaattcgtc gaggtcggca tttacacctt tttgaataat
     6781 atttccccga ttaatgagta tgggagggtc gttttgaatt tctctttcaa ttttttcgga
     6841 aataatggag caggcattca gctggtcggc aatggtgcgc aaagtgatgt tttctgtatg
     6901 agtacacaat tgttttattg gttcgatggc tttgagcgcc gttttcaatt gaaccacttc
     6961 gcgaggattg attctgccca cagcaatttt agaaaccaga cgttccaaat cgccaatcag
     7021 cgcaatattc tgagccaaag cttctttcaa ttccggatgt ttgaaaaaat attctactac
     7081 atccagcctt tcgttgatag attttacatt tttcaacgga aaagccatcc aacgtttcag
     7141 cagtcgactg cccatcgggc tgatggtttt atccagcact tcgagcaagg tttttcctcc
     7201 ttcgttgata gcttcaaaaa gttcaaggtt tcttacggtg aatctgtcaa gccatacaaa
     7261 gcggtcttct tcaatgcgag agagttgggt aatatgtccc gtttgttgat gatgggtcag
     7321 gtcgaggtaa taaagaatgg caccagctgc aaccactccg ttggcaaatc cttccacacc
     7381 aaaacccttc aaacttttcg tttcgaattg tttcagcaac ctgtctttgg ctgcatcaag
     7441 ggtaaatacc caatcttcga gcggatagga ataaaatttg gttccaaata aattttcaaa
     7501 ttctttcttt ttggtacggt cgaaaataat ttctttggga gagaagctgt tcagcaattt
     7561 atctacgtat tcgtttgttc cttcagccaa cagaaattct ccggtagaaa catcaagaaa
     7621 ggctacacct gcctgatttt tgttgaggta aacgctcgct aagaaattgt tttccttgtg
     7681 gttgaggatg gtgtcgttgt aagtaacacc tggcgtaacc agttcggtga tgcctctttt
     7741 tacaattttt tttgcaagtt tgggatcttc cagttggtcg caaatggcta cccttttccc
     7801 agctctcacc aattttggca gataagtatc caacgcatga tggggaaatc ctgccaattc
     7861 tacgcttttt gccgatccgt tggcacggcg cgtgagtgta attcccaata tttccgaagc
     7921 ttcgatagca tcagttgaga aagtttcgta aaaatctcca caacgaaaca ataaaatggc
     7981 atcgggatgt tttgatttca tctcgaaata ctgtttcatt agaggcgttt ctacaattgg
     8041 ttcagacata tatcggcaat ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nntttttctc tatgttctac taattgaaat tatgaaaagt cggggcaggg gaaaaatttt
     8221 tcagagcttg aaaaaaagtt tgttgatggc agcagtcatt ttatcccaac cgtatttcat
     8281 cttttctgtt tttatgttgg gcaaaaatat ttcttcccgg ttgttctgaa aaaaatcaac
     8341 tatggcgtcg gcaatttctt tgggttgtcc gtttaccaca tagccaactt ttccatttgg
     8401 gataatttcg gccaagcctc ccacattggt aaccaacatt ggtttttcaa agtgataggc
     8461 aatttggctc actccactct gagtggctga acgatagggc tgtgcaacca aatcggcaag
     8521 actgaaatag tatttaactt cattttcagg aataaaattg gttcttagtt caactctgtt
     8581 ggccagtccc aattgtgcaa tctgttttaa ataaggctcg ggattttcgt aaaattctcc
     8641 ggcaacaacc agttgcactt ccatatcttt caatctttta tcggaaaaag cttccaggag
     8701 taaatccaag cctttgtaat ggcgaatgaa tccgaaaaac aacacatatc tttttttggg
     8761 gggcaagccg agtttcaatg ctgcttcgct tttcgataca attgcaccgt aatggtcgta
     8821 aacaggatga ggaacaacaa tttttggttt attttttttg tcaaaatgtt gaatatcttc
     8881 tttcactgaa tttgccattg ccagaaaacc atccatattt tttacgaaat atcccggcag
     8941 ccacttgtcc agtggtgttg cttcatgagg aatcatattg tgaaccaaac cgagcatttt
     9001 agtttgtgga gaacgagcat atcgtgcgat agttgcaaaa cagggtgcta taaaagaagt
     9061 ccagtagcaa aatatgacca aatcgggagc tttcttcttg atttctttcc caatctttat
     9121 ccagttaaat ggatttatcg aattgatacg gcggataatt ttaaggtctt cgggtggttt
     9181 ttccgttgaa tattgcgatt ttcccggaaa cagaaaattt ggatattgaa gagtgaaagt
     9241 gatcaattcc acttcatgac cttctctggc gtattcacgc gccaaacgct cgttgaacga
     9301 agccaacccg cctcggtaag gataggctgt gccgacaatt atgattttca tatgtaagtt
     9361 tttcaaatta caaaattaca aaaattccga cgaataaaat ttcggttttt ttgttaaatc
     9421 taaaactcat agaattatca agagcaaaaa ttatcaaacg gtctatctga attgaaacgg
     9481 gaagaaaatt ttttaccttt gctttcaaaa ttcatatttt attgaaagga taaacaaatg
     9541 gaagaaaatc aggatattaa atatatgaaa caagcgcttg cggaagcacg gcgagcagcc
     9601 gaaaaagatg aagttcccat aggtgccgtc attgtatgtc agggaagtat aattgctcgt
     9661 gcccacaatc tcaccgagac tttgaatgac gttacggctc atgccgaaat gcaggctatt
     9721 acttcagctg ccaattattt gggaggtaaa tatctgtccg actgtaccct gtatgttacc
     9781 atagagcctt gcgttatgtg tgccggagcc ctgggatggg cacaaatcaa gaaaattgtt
     9841 tacggagctt ccgacgaaaa acgcggatat tcaaaatttg ctccagatgc tcttcaccct
     9901 aaaacagaag tagtgtctgg agtgttggca gaagaatgtg ctacattgat gcagaatttt
     9961 tttaaaagaa aaaggtaaac acattttgag aaatagaaga gtattttgta ctccaaaatg
    10021 tttttacctt ccttccacaa ttgttttgat cttgttatac ggcagatttt atttgctgca
    10081 tcaaggtgtt cttatctatt gctccggaat gtttccaaat gacatttcct tttttgaaca
    10141 gcataaaggt aggaactgcc tgaatcccat atttggcagc caatgcctga tttttgtcca
    10201 catccacttt tagcacacgg gcttgcccct gaacttcttt gcccagactt tcaatcaccg
    10261 gagccatcgc tttacatggt ccgcaccacg tcgcaaaaaa atctaccaat accggtttat
    10321 ctccgttgat aatatcctga aatgtttcca tatatttttc tttcttgttt cgatagtaaa
    10381 aaagctgatc taaaaaatct aattttttcc ttttgttatt gaatatcaat cgtccgcgcc
    10441 ggctttactt tggcttcttc ttttttcgga atggtgattt tcaaaattcc gttttcatat
    10501 actgccgaaa ttttttctgc atcaactgtc tcgggtagag taaatgttcg taaaaacgac
    10561 tgataactga actcgcgctt tgtatattca ccttccgttt tttcttcatc aagttttttg
    10621 ttggaagaaa cggtcagcaa atcttcgttc atttcgattt taatatcgct tttttcgtaa
    10681 ccgggtgccg ccacatcaat ttcgaaagct tcaccatttt ctttgatgtt aactgccggt
    10741 aaagtagtat gatttgctgc caattcccgg tcagtccagt tataatcttc ccattcgtta
    10801 tcgaaataac ggtcaaaatt tgatggtaat tgttcggtta gtctgttagt tgccatatat
    10861 cagccctcca tcactttgat tggttaaaca tatttttttg ctcctaagtt aaaaacaaaa
    10921 taaataccaa acgtggaaat tagtcatttt ggcaatttca actggctgaa atatggtcat
    10981 ttaggcagaa aaatcaaata agctgaagaa aaatttgaca agaactatcc ttattctctc
    11041 cattcctgaa aacgcatgga ttggggaatg agtcgggtgg attgtaaaga tttttcattt
    11101 gtgatggaga aatctatgct ttcgcataaa ctgactatag tcaacagaga aaacatatta
    11161 ataaactatt attcaattat ttagaaatta aaattgacaa attctgattt ttgtcggtta
    11221 atagtgatta aaattatttt gtttttgaaa attttaatat tatttttgtg gttttgcata
    11281 agttatttta tttgttgcga atacaagttt taatattaaa ttctaaattc agttggaata
    11341 aatcttataa ccatgaaaaa atttattgtt ggctttttca ttttgtccat tttcatctcc
    11401 tcttatggac aaccaaatga ttacaggcga tggtcaataa ccttggatgc aggaatcaat
    11461 aattttgacg gagacaataa tcaaaattat aaccagatta taccgaattc tgcagcaaag
    11521 gcaagctttg gattttcaac agagcttact cttagtcctt tttggggcat tggaactgaa
    11581 tttttttatt ttcccgtttc tgcagaagat gctgccaaaa aattcaacgc agatgtattt
    11641 catttcactc cttacctttc gttgaatctg cttaatttat gtaatgaaaa taatcattcc
    11701 aaatggaata tttatgctac ggtaggcgct ggagtggcct attacaattc tactttgtat
    11761 aaaaatggca ttgagtttga cagaataaga gatggatggg ctgcagtttt cccggtagga
    11821 ggtttgatag agtataatat ctcgtcacta tttgctatag gtgcaaaagt gcagtatcgt
    11881 ttgcagaata aagataactt tgaaggaacc aatttgaaag aagaagccgg aggatataat
    11941 tttaaaggtg ttaccaacga caatattgca atggcagctc tttcgttgcg ttggaaaatt
    12001 ggagcaaaaa gtaaaaatca catacgcaac aacgaaacaa gaattacttt aggagatgtt
    12061 ttcactttag caaatcgggc aaacatcaaa acagattcgc tggctcaaga actcgataag
    12121 ttgaaattaa gtttgcaacc gggaccggat gatgacggcg atggcgtccc caatagcagg
    12181 gatttggagc caaatacacc aaaggggctt tacattgatt ataacggacg tggcattcct
    12241 gaaataaaag taatccccga aaccaaagag attcatcctg aatatcttca atatataagt
    12301 gcttcaacaa aagatgaaga taaagatggt gtccccgaca accgagacaa agagcctaat
    12361 tcaccaaaaa atctgtgtgt cgatttttgg ggtagaagca tagctcaaga taaaccatgc
    12421 gcttttgcct ctgtgtattt tgactttgac aaaaccaatt tggatgaaga agcattaata
    12481 gaaattgaaa aactggcaga aaaattaaat actgatccaa ccttattggt agaaatcaga
    12541 ggatatgccg ataatgtggg aagtaacaaa tacaacaaaa gattaagtca ggaacgctcc
    12601 aatcgcgtaa aagcggaatt ggttttcacc cataacatca atccgcagag aattatttcc
    12661 aatggaaagg gaaaaatccc tcaacctccc agagcctata gaatgaatag aagatgtgat
    12721 tttattttga gcaaataatt tagattatct atttttagag ttggaagttc ttacggcgct
    12781 aattttattc aatcaattca tttataggta tttacaatta taaaaagatc actttcaaaa
    12841 tcaaaattgc taaaatgtaa caatttgatt acttttgcag ccaacttatt tatcattaac
    12901 aaccttcata ccggtttatt ttcaattttt cccggtacga ttaaaattaa agactatgag
    12961 taataattta ttagttggaa aaagaggaat catctttgga gctctgaacg atatgtcgat
    13021 tgcctggaaa gttgccgagc gcgttgtcga agaaggagct accgttaccc tgtccaacac
    13081 gccaattgcc atacgtatgg gaaccataaa tgaattggca gaaaaaatca atgccaaaat
    13141 tatccccgcc gatgctacca atcttgatga tttggagcaa gttttctctc aatcgatgga
    13201 aattttgggt ggtaaaatcg actttattct ccattccatt ggcatgtcac caaatgtgag
    13261 gaaaaagagg ccctatgatg atttggatta tgatttgatg cacaccacat tggatatttc
    13321 agccatatct tttcacaaaa tgcttcagac tgccaaaaaa atgaatgcca tcaatgagtg
    13381 gggttcggtt gttgctcttt cctacattgc ttctcatcga acgatgtatg gatacaacga
    13441 tatggccgat gccaaagcca tgctggaatc gatagcccga aacttcggat acatttacgg
    13501 aagagaacac aatgtgcgta tcaacactat ttctcagtcg ccaactccta ctactgccgg
    13561 aagcggagtg aagggatttg acagtttaat ggatttttcg gaacgcatgt cgccactggg
    13621 aaacgcttca gcagatgatt gcgccaatta ttgtgtagta atgtttagcg atttgacacg
    13681 caaggtaacc atgcaaaacc ttttccacga tggaggcttt tcgagcatgg gtatgagtta
    13741 tcgtgcattg cagcagtaca acaaaagctt tgaaggaagt caaactcacg atggcaaaat
    13801 tatttacggt taatccaaca attgaattat aatactaaaa agggacaatc tactttcaaa
    13861 gtaaattgcc ccttttttta aaattcaatt tagaagttaa tcttcttctt tcttagaagc
    13921 ttcatattct ttcaacagtt tttcctgaat ttcgatagga acgagttcgt aacttgcaaa
    13981 tttcattaca aacgaagctc ttccaccggt taaagaactc aaggttgtgg aataattact
    14041 cagttctttc aaaggaattt ttgcttgcaa cttttcaaaa cctttttcgc tgttcatacc
    14101 catgataacg gcacgacggc tttgcaaatc gctcatcaca tctcccaatc tgtccgatgg
    14161 agtcattacc accacatcat aaaccggttc cagcaattta gggccggcgt ccttgaaagc
    14221 ttgtgcaaaa gcatttcttc cggctaatcg gaaagaaatt tcgttggaat caaccggatg
    14281 catttttccg tcataaacaa tgacgcgaac atcacgcgca taagaacccg ttagtggtcc
    14341 ttgttccata cgatccatta cacctttcag gatggccggt aaaaatcgtg catcaatggc
    14401 accgcctaca atactgttga caaataccag ctttccgccc cattccaaat ctatttcctg
    14461 aacatcacga acgcttattt tgtattcctg accattgaaa cgatacacat cctgaatggg
    14521 aacatcttcg aaatacggtt caacaattag atgaacttcc ccaaactgtc cggcaccgcc
    14581 ggattgcttt ttatgacggt aatcggctcg ggcttttttg gttatggttt cgcgatacgg
    14641 aatttttggt tctttaaatt caatttctat tttgtcgttg ttttcgactc tccatttcaa
    14701 ggtgcgcaag tgaaattctc cctgaccatg caaaatggtt tgtttcagtt ctttcgactg
    14761 ctctactatc catgtgggat cttcttcgtg cattcgagtg agtatttcgt tcaatttttc
    14821 ttcatcagct tccgatttgg ctttaatagc ccggcggtat ttgggttcgg gatattctac
    14881 tccgacaaaa tcataatcgc aacccttgcc gttcaaagtg tttcctgtgc gggtatcctt
    14941 cagttttacc gttgctccga agtctccggc tgaaatttct tccaccttgt tgcgaatttg
    15001 cccggcaacg cagaaaatct gactgattct ttcttttact tggcggttta cgttgtaaag
    15061 gtcatctcct tccttcaatt ttccactcat aactttaaag agtgaaattt ctccaatatg
    15121 cggctcaacg tttgttttaa aaacatagat actcgttggc ccatctggat ttggatctac
    15181 actttttcca tctttagtca aggggcgttt gctgtcggaa acggagggag caatccgcac
    15241 caaataatcc atcaaagtac ttacaccggc atcattggcg gcagaaacgc aaattaccgg
    15301 aaaaattccc ctttccaaca cgcctttata caaaccttta taaatttctt catccgaaag
    15361 tattccttct tcaaaaaatt tttccattag actttcatca aattctgctg cagattcaat
    15421 cagactattg tgaagctctt gagcttttgc tttttcggag tcgggaattt cggtaatttc
    15481 aggtgctcca ccttcagttt tgaaacgaag cattttcatt tgcagtacat caaccaagga
    15541 attaaatcca cttcctgtct gtaatggata ttgcagctga ataactttat ttccaaaatt
    15601 ttccttcagt tgttcaatgg tatgttcata atcagctttg tcgtgatcca actgattcac
    15661 aacaaacatg acaggtttgt tgtatttttc ggcataagag aaatggttgt tggtgccaac
    15721 ttccactcca tattgagctc ctacaacaat taatcccata tcggtaacat taagtgatgt
    15781 taccacagca cctataaaat cgtcagaacc cggacaatca atgatgttaa gtttatgttg
    15841 acgccattca atctgtgcca gtgttgggaa taccgaatat ccatattcct tttctaccgg
    15901 aaaatagtcg catactgtgt tctgattggc aatggtgcct ctgcgtttaa tgattccacc
    15961 ctcgaaaagc atagcttcca caagggtggt tttacccgac ccagaacttc ccaataaaga
    16021 aatattcttt atttcattag gctgatatac tttcataact tattaatttt taattgatta
    16081 atactataaa attatgtttt aactttgtaa aaatcgaatc gacaaggtat aaaaagtcca
    16141 ttaatttgga gataaaattt ttatgttata aaacattgtt ttaaaacaca agttaaatca
    16201 ttgaatttca aaagaaaaat cggcataaaa attaatttcc ggttaatgaa attattgttt
    16261 tcccagcaaa tcgttcatct tccatgaaaa atctttaata gagttgaaac agaaatgctg
    16321 ttaatcgatg tcaatatgtt cagccagaac gggttcgttt agaaaaattg tagcagaagc
    16381 cgaaaatttt tccaccgatt cggttgtcag aaaccgacag gaagcatttt tagtgcaaag
    16441 tgcgtcgatt tcgggatgtc ggttcaggta atc
//
LOCUS       DAOSIB010000047        16425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_3703_length_16425_cov_12.427673, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000047 DAOSIB010000000
VERSION     DAOSIB010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16425
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3703_length_16425_cov_12.427673"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1795)
                     /gene="hcp"
                     /locus_tag="PLD44_05530"
                     /gene_synonym="priS"
     CDS             complement(140..1795)
                     /gene="hcp"
                     /locus_tag="PLD44_05530"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOF98829.1"
                     /translation="MFCYQCQETSKGTGCELKGVCGKTSDVAALQDLLIYQLKGLSHY
                     TTALRKVGVESPLVSRFIIDGLFMTVTNVNFDKQRFVEQIREGFGLRSQLKQDLSTHG
                     ITLSASELTEWFADTEADMENMASKVGVLSTENEDVRSLRELIIYGLKGMAAYAKHAM
                     NLRFTDGEINAFVEETLVKTTDDTLSMEELINLVLETGRFGVKVMELLDKANTTAYGH
                     PQVTKVSLQTREKPGILISGHDLKDMEELLKQTEGTGVDVYTHGEMLPAHYYPAFKKY
                     DHFVGNYGGAWWNQTQDFETFNGVILFTTNCIVPPRSNSTYADKVYTTGAAGFPGFRH
                     IAEAPEGKAKDFSSLIEHAKQCAAPIQIEKGEIVGGFAHEQFFALSDQIIEAVKSRQI
                     RKFFVMAGCDGRLKSRNYYTEFAEKLPKDTVILTAGCAKYRYNKLALGEINGIPRVLD
                     AGQCNDSYSLALIALKLKEIFNLNSINELPIVYNIAWYEQKAVIVLLALLYLGVKNIH
                     LGPTLPAFLSPNVAKVLVDNFGIAPIGSVEDDLNMFLNLENFN"
     gene            complement(1856..2212)
                     /locus_tag="PLD44_05535"
     CDS             complement(1856..2212)
                     /locus_tag="PLD44_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOF98830.1"
                     /translation="MENSEANNRQESKTIPAGEKFILANQVNYADGSVVSKVIVRNRQ
                     GNITLFAFDKGEFLSEHAAPFNALVVALDGKGTITISGQPFELISGESIILPAGVPHA
                     VNATERFKMMLVMIKD"
     gene            2382..3044
                     /locus_tag="PLD44_05540"
     CDS             2382..3044
                     /locus_tag="PLD44_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOF98831.1"
                     /translation="MKSIDPEWKSYGSIDDGESGFPADLKYSIKPYRKNEIMFRQGSI
                     CDALYILTSGSVKTEMITETGNIMGIELIKAPRPLAPAFLFSDNNRFPVDVTALEDCE
                     ILRIPKEEVMRLMAIQPDFMQQFLKHNANRAEFLANRLQLLSIKTIKGRIAHFLLEQM
                     AEYGKTFTINRNRTELAEFFGVARPSLARSLSEMVEDNIIKINKKQFTVIDERKLKDL
                     LL"
     gene            complement(3079..5925)
                     /gene="gcvP"
                     /locus_tag="PLD44_05545"
     CDS             complement(3079..5925)
                     /gene="gcvP"
                     /locus_tag="PLD44_05545"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOF98832.1"
                     /translation="MKDFLSFRHIGVSSDEIQEMLEVVGAESLDELITQTIPLSIRLN
                     EKLNLPSALTENQYAQHISEIAEKNKIFTSYIGRGWYDTCTPAVILRNVFENPSWYTS
                     YTPYQAEISQGRLEALLNFQTAVCEFTGLPLANASLLDEATAAAEAATMMYNLRSREQ
                     MKNGANLLFVDENIFTSTLAVLQTRTQAQGIQLEIGDYKSVQFSEKHFGAILQYPNAD
                     GNVEHYFSFVSQAHTAGCKVAVAADILSLALLVPPGEWGADIVFGSTQRWGIPMGFGG
                     PSAAYFATRDEFKRDMPGRIIGISKDKNGKPALRMALQTREQHIKREKATSNICTAQA
                     LLASMAGFYTVFHGPEGIRNIAKNIHAIAAYINAMLPTYGYRQLNKLFFDTLKIVLPE
                     NVSQEEFRNIALSHKVNFYYFETGEVGLSINETTEAPDIQRLINIFSEAVHKKPIVFN
                     PTDLSEFEIPDEKIRRKSAYLTQEIFHRYHTEMEMMRYIKKLERKDISLTHSMISLGS
                     CTMKLTAASEMLPLSKAEFLKIHPLAPSDQVKGYIELIGELEKYLCVITGFDACSLQP
                     NSGAAGEFAGLMTIKAFYRSRGETERNVVLIPASAHGTNPASAHQAGLEIVVVDCDEN
                     GNIDLNDWKVKAETYRTRLLGCMVTYPSTHGIFEAHIGEMCQIIHANGGQVYMDGANM
                     NAQVGLTHPAKIGADICHLNLHKTFAIPHGGGGPGVGPVCAAAHLAPFLPGKTSGQNT
                     VSAAAYGSASILPITYGYIRMMGAEGLTNASKIAILNANYLAERLKETYGVVYRGENG
                     RVGHELILECRNFKVVGITETDIAKRLMDYGFHAPTLSFPVHGTLMIEPTESESKAEL
                     DRFVEAMIQIYEEIMEIENGTGDKTDNVLLNAPHPEYEMVSDEWKHLYSRKKAAYPTK
                     WTAENKFWINVARIDNAFGDRNLICSCPPTVEWQ"
     gene            6686..7798
                     /locus_tag="PLD44_05550"
     CDS             6686..7798
                     /locus_tag="PLD44_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOF98833.1"
                     /translation="MIDTLNLFGKTKIEVPKTEGIKYAGSKLKILPYIFNMISGLDVE
                     NILDGFSGTTRVSQALAKTGFKTTSNDISIWSATFAKAYLLNNRKPEYYLPIIEYLNS
                     LKGYVGWFSEYYGGTENNKEKRPFQLKNTMKLDAIRDEIDRMNLDEIEKSVALTSLIL
                     ALDSVDNTLGHYAAYLAEWSPRSYRDLKLKLPLLFKNNQQNEVIKGDIFETVKNHKFD
                     LAYFDPPYGSNNGKMPPSRVRYNSYYHIWTTVILNDKPKLFGKVNRRLDSKDEVAASV
                     FEEFRKDEQGHFIAMNAIKELIHCTNSKYILLSYSSGGRATKEELFDIINSEGKMIQA
                     IEINYKKNVMGQMRWTNEWINSDDKYMEYLFLMEKC"
     gene            7891..9213
                     /locus_tag="PLD44_05555"
     CDS             7891..9213
                     /locus_tag="PLD44_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOF98834.1"
                     /translation="MITSEPIIHVGILSSREIAFILNGIFIISDGKNFSGKQKAEWKN
                     GKILFDGETFDELNFQPHDESSTFDLLDVVIGIHFHWERKENQRFKGALKIIVENEQL
                     TVVNEIKAEDYLLSVISSEMSATSSLELLKAHAVVSRSWLFYPLQKDKKELSGRESVE
                     ENTENRHIKWYERGEHEHFDVCADDHCQRYQGITRATSDAVRHAVEATRGEVLMFEQQ
                     ICDARFSKCCGGVTETFDNCWAPEEHPYLSSIVDNDLLPQSFDLDLTMEENAQKWIRE
                     FPTAFCNTQDKNVLTQVLNNYDQETTDFYRWKVVYTQEELAEILRRRSGYDFGDILDL
                     IPLKRGKSGRIVELKIVGTKRTMIVGKELEIRKWLSDSHLYSSAFVVDKENYSQQIPG
                     KFILTGAGWGHGVGLCQIGAAVMGEKGYAYERILLHYFRGAELKRIYE"
     assembly_gap    9611..9710
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9711..10698
                     /locus_tag="PLD44_05560"
     CDS             <9711..10698
                     /locus_tag="PLD44_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF98835.1"
                     /translation="NYKEVVVFNPFSHNAVYQRYYLVRDTDVSVPADGLKIKIPVRSL
                     AATSVTHFEFLNLLHVIDAVTGVCSSKIIYSPQINERLNKGLIVDLGDAFNINLEKTV
                     QLHPDLVMVSGYNQNDPFAQRLLQAGIFPVYNNEWMEKSLLGRAEWIKFVAVFFDKEK
                     IADSLYLEIEKEYQNTANRVKGIKFRPQIMSGSNFRGTWYMPGGRNFMAQLFSDASGN
                     YFYANDTTVGSLPLSFEKVLKNFSESDVWLNCNYASLSELLQADPRHALFRPVKLQQV
                     YNFNKRMLPSGANDFWESAVAHPDLLLKDVIFILHPELLPGYEPFYTQKLNP"
     gene            10706..11701
                     /locus_tag="PLD44_05565"
     CDS             10706..11701
                     /locus_tag="PLD44_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOF98836.1"
                     /translation="MYSRNHLLFFAFSLLFIFLFLADLAWGSVAIPAKEIYSVLLGKQ
                     SSSIYTEIIVNFRLPKAITAILAGASLSVAGLMMQTLFRNPLADPYILGVSSGASLGT
                     ALVVMASGFLPFSFVGNGWALIISAIVGAALVLLIVLFVSFRVNDIVSLLIVGIMFGT
                     IAAALVSIIQNFSNPDAIKLFLMWTFGSLSAVTWNYLQILLPVVLVGIGIAFFLQKRL
                     DGLLLGENYARGLGIHISQTRSLIILATGLLAGGITAFTGPIAFVGVAVPHIARGIFQ
                     TSSHKILLPACMLVGAALLMICDIITQIPDYTLPVNTISALFGAPVIIWIILRRK"
     gene            11729..12718
                     /locus_tag="PLD44_05570"
     CDS             11729..12718
                     /locus_tag="PLD44_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF98837.1"
                     /translation="MSVLSTQHLSIGYRRKGTANVIQHDLNLKLESGKLVGLIGPNGS
                     GKTTLLRTIAGLLKPIDGNILIDDKEFLLLSQREKALNVSFVLTEKVDIPNADVFDMV
                     SFGRFPYNNWRGNLSKQDRTKVFQALQMVRMEKMMHRYINELSDGERQRVMIAKALAQ
                     DTPIILLDEPTAHLDVPNRVGIMLLLHKLAHKTGKMILLSTHELDLALQAADLIWLMS
                     KNGGIECGVPEDLVFSGSFNRTFASDYYFFNEANGNFSLNYPLTGKVSVSGNKMRIYW
                     TLRALARAGYEAVENAPLKIEVTDSAWILNQKEIFSVAEMLDELNNLNGNTSL"
     gene            13105..13302
                     /locus_tag="PLD44_05575"
     CDS             13105..13302
                     /locus_tag="PLD44_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOF98838.1"
                     /translation="MSKKLTRSSNQMIAGVCAGIAEYFGWNVTLVRVIYVLLSIFSAG
                     FPGTMVYIILWIVMPRPEKFD"
     gene            13376..15394
                     /gene="uvrB"
                     /locus_tag="PLD44_05580"
     CDS             13376..15394
                     /gene="uvrB"
                     /locus_tag="PLD44_05580"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOF98839.1"
                     /translation="MNFKLESPYQPTGDQPEAIQQLVEGINAGIPYQTLLGVTGSGKT
                     FTIANVIEQVQRPTLVLSHNKTLAAQLYSEFKSFFPHNAVEYFVSYYDYYQPEAYLPL
                     TDTYIEKDLSINQEIEKLRLSATSSLLSGRRDVVVVSSVSCLYGIGNPDDFSKNVIEI
                     RKGQHLVRNAFLRNLVNSLYTRNEIELNRGNFRVKGDTVDIFLAYTDFILRIVFWGDE
                     IDEILTVDPVNYDIIDSLDAYKIYPASIFVTTKERIAVAINEIEKDLQLQVDYFRSIG
                     KDLEAKRIYERVRYDIEMIKELGYCSGIENYSRYFDGRPPGSRPFCLLDYFPKDFLLV
                     IDESHVTIPQIRAMYGGDASRKQNLVDFGFRLPAAKDNRPLRFEEFEQLTPQTIYVSA
                     TPADYELVKSEGIVVDQLIRPTGLLDPVIEVRPSLNQIDDLLEEIAQRTENNERVLVT
                     TLTKRMAEELTNYLSKMGVKCNYIHSDVETLDRVQIMEDLRRGVYDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRSHRSLTQTAGRAARNLNGKVIMYADQITESMEKTINET
                     NRRREKQLRYNEAHHITPTAIVKGERNPLSKAEPYAYVEPEKSTDIAADPVVRYMSVP
                     ALEKAIAKTRKAMQEAAKKLEFLEAAQLRDELMKLEELLKKKMFEAKN"
     gene            15573..16193
                     /locus_tag="PLD44_05585"
     CDS             15573..16193
                     /locus_tag="PLD44_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOF98840.1"
                     /translation="MLEIIPKGILIGLCISVPVGPIGMLVIQRTLNRGRKYGVATGLG
                     ASMSDLFYTIITLFFLSFVISFIEAHRFVIELIGSLVVICFGYFIYKNNPSVQPKPNE
                     TVQHNVYRDFISSFVLTFSNPLILFILIALFARIGFIDSETSFFQTIIGIISILIGAT
                     IWWLTLTFIVSHFRSKIGMHQLKAINRITGGLIALIGCIGLVSSLI"
BASE COUNT         5009 a         2986 c         3241 g         5089 t
ORIGIN      
        1 tttttgttga aaaaatgaaa attacatttg taatttcctt aaaattttct atatcccatt
       61 tatactgaac gggtaatcaa aaaaatttag gtagctccaa aaacaagatt ttgccgaata
      121 ataaaacttt gtgaaataat caattgaaat tttctaaatt gaggaacata ttcaaatcgt
      181 cctccacgct gccaatagga gcaattccaa aattatctac caacactttg gcaacatttg
      241 gtgaaagaaa tgccggcaat gtggggccca aatggatatt tttcaccccc agataaagga
      301 gcgcaagaag cacaattaca gctttctgtt cgtaccatgc gatgttatac acaataggca
      361 attcattgat actgtttaaa ttaaaaatct ctttcagttt cagggctatc agtgccagcg
      421 aataactgtc gttacactgg ccggcatcga gcactcgcgg aatgccattg atttctccca
      481 atgcgagttt gttatagcga tattttgcac agccagccgt aagaataacg gtatcttttg
      541 gcaatttttc cgcaaattcc gtataataat tgcgactttt taatcttccg tcacaaccgg
      601 ccatcacaaa aaatttccta atttgtctgg atttcactgc ctcgataatc tgatccgaca
      661 gagcaaaaaa ttgctcgtga gcaaaaccgc ccacaatttc tcctttttca atctgtatgg
      721 gagcggcaca ttgtttggca tgttcaatca acgacgaaaa atcctttgcc tttccttcag
      781 gtgcttcggc aatatgtctg aagcctggaa aaccagccgc tccggtagta taaactttgt
      841 cggcataagt ggagttgctt ctcggtggaa caatacagtt ggttgtaaaa agaatcactc
      901 cgttaaatgt ttcaaaatcc tgtgtttggt tccaccatgc tccgccataa tttccgacaa
      961 aatggtcgta ttttttaaat gccggatagt agtgtgccgg caacatttct ccatgtgtat
     1021 aaacgtcaac gcctgttcct tcggtttgtt tcagcagctc ttccatatct ttgaggtcgt
     1081 gtccactgat taagataccc ggcttttccc gagtttgcaa actgactttt gtaacttgtg
     1141 gatgtccata agcagttgta ttggctttat cgagcagctc catgactttg actccgaaac
     1201 gtccggtttc caacaccaga tttatcagct cttccatact caatgtatcg tctgtggttt
     1261 ttacgagtgt ttcttctacg aaagcattta tttcgccgtc ggtgaaacgg agattcatgg
     1321 catgtttggc ataagcagcc attcctttca atccataaat tatcagttcg cgcaaagagc
     1381 ggacgtcttc attttcggta ctcaacacgc caactttgct tgccatattt tccatatctg
     1441 cttcggtatc ggcaaaccac tccgtgagtt cggacgccga caatgttatc ccatgagttg
     1501 ataaatcttg ttttaattga gaacgcaggc cgaatccttc acgaatttgt tcaacaaatc
     1561 gctgtttatc gaaattgaca ttggtaaccg tcataaagag gccgtcgata ataaaacgac
     1621 tcacaagcgg actttcaact cccactttgc gcaaagcggt tgtgtaatgt gataatcctt
     1681 tcagctgata aatcaacaaa tcctgcaaag ccgccacatc ggaagttttt ccacaaactc
     1741 ctttcaattc gcagcccgtt cctttagaag tttcctgaca ctgataacaa aacatattca
     1801 ttttttttca caattaattt taaaatctca aaatctattt ttatttactt gtttttcaat
     1861 cttttatcat caccaacatc attttaaaac gttctgtggc attcaccgca tgtggaacac
     1921 cggcaggtag aatgatactt tctccggaaa ttaattcaaa cggctgcccc gaaatggtaa
     1981 tcgttccttt accatcgaga gctaccacca atgcattgaa tggtgctgca tgttcactta
     2041 aaaattctcc cttgtcgaaa gcaaacaggg taatgtttcc ctgacgattt cgaacaataa
     2101 ctttgcttac caccgagcca tcggcatagt tgacctgatt ggctaatata aacttttcgc
     2161 ctgcaggaat tgttttgctt tcctgtctgt tgttagcttc tgaattttcc atcatgtttt
     2221 gaatttttag ttatgtaatt ttataaattg tttttgaaat tgaagcacaa tactttatat
     2281 ttgtacactg tacgagattg aagtttttga cattgcaaag ttagagagtt taaaacaatt
     2341 aaaatgtaac acttgttaca gatgaaaaaa attgattttt catgaaaagc attgatccag
     2401 aatggaagag ttacggcagt attgatgatg gagaaagtgg tttccctgcg gatttaaaat
     2461 acagcattaa gccataccga aaaaacgaaa ttatgtttcg tcagggaagt atttgcgatg
     2521 cattgtacat tttgacctcg ggttcggtga aaacagaaat gattaccgaa accggcaata
     2581 ttatgggaat agaactgata aaagctcctc gtccgttggc tccggctttt ttattttccg
     2641 acaacaaccg ttttcccgta gatgttactg cactcgaaga ctgcgaaatc ctacgtattc
     2701 ccaaagaaga agtaatgcgt ctgatggcga tacagcccga ttttatgcag cagtttttaa
     2761 aacacaatgc caaccgagca gagtttttag ccaatcgact tcagttgctt tccattaaaa
     2821 ccataaaggg acgcattgct cattttttat tggaacaaat ggccgaatac ggaaaaactt
     2881 tcactatcaa ccgaaacaga accgaattgg ccgagttttt tggcgtggca cgtccttcat
     2941 tggccagaag tctttcggaa atggtcgaag acaacatcat taaaatcaac aaaaaacagt
     3001 ttactgtaat tgatgagcgc aagttaaaag atttgctcct ttaatgttaa aaacttaata
     3061 aattgcgaca ataaaaatct attgccactc aacggttgga ggacaggaac aaattaaatt
     3121 cctatcacca aaagcattat caatacgtgc gacatttatc caaaatttgt tttctgcagt
     3181 ccatttcgta ggataagcgg ccttttttct gctgtaaaga tgtttccatt catccgacac
     3241 catttcatat tccggatgag gtgcattcaa taaaacattg tccgttttgt cgcctgtccc
     3301 attttcaatt tccataattt cctcataaat ttgaatcata gcttctacaa acctgtcgag
     3361 ttctgctttt gattcacttt cggttggctc aatcatcaac gtaccgtgaa caggaaaaga
     3421 caaagtagga gcatgaaatc cataatccat caaacgtttg gctatatcgg tttccgtaat
     3481 accgacaact ttaaaatttc gacactccaa aatcaattcg tgccccacgc gaccattttc
     3541 gccccgatac acgactccgt aagtttcctt taaacgttcg gccaggtagt tggcatttaa
     3601 gatagcaatt ttagaagcat ttgtcaaacc ttcagctccc atcattcgta tataaccata
     3661 cgtgatgggc aaaatactgg cactgccgta agctgcggct gaaacagtat tctgaccgga
     3721 tgttttaccc ggcaaaaaag gtgccaaatg agcagcagca caaacgggtc caacaccggg
     3781 accgcctcca ccgtgaggaa tggcaaaagt tttatgtaaa ttcaaatgac aaatatccgc
     3841 accaattttt gccggatgag ttaatccgac ctgagcattc atattggctc catccatata
     3901 aacttgtcct ccgtttgcat gaataatttg gcacatttcc ccaatgtgag cttcaaaaat
     3961 gccatgtgta gaaggataag ttaccataca acccaacaat cgcgtacgat aagtttctgc
     4021 tttcactttc cagtcgttga gatcaatatt cccattttca tcgcaatcga ccacaactat
     4081 ttcaagcccc gcctgatgag ctgaagccgg attggtgcca tgagccgatg ccggaattaa
     4141 aacaacgttt cgttctgttt caccacgcga ccgataaaag gctttgatag tcatcaagcc
     4201 ggcaaactct ccggctgcac cggaattggg ctgcaaactg caagcatcaa aaccggtaat
     4261 aacacacaaa tatttttcca attcgccaat tagttcaatg taacctttca cttgatcgga
     4321 cggagccaaa ggatgaatct tcaaaaattc tgctttgctc aacggtagca tttctgaagc
     4381 tgccgtgagt ttcatggtgc acgaccccaa tgaaatcatg gaatgagtca acgatatatc
     4441 ttttcgttcc agtttcttga tataacgcat catttccatt tcggtatggt agcggtgaaa
     4501 aatttcctga gtcagataag ccgactttcg tctgattttt tcatccggta tttcaaactc
     4561 cgacaagtcg gttggattga agacaatcgg ttttttatga acggcttccg aaaaaatatt
     4621 tatcaatctt tgtatatcgg gtgcttcggt agtttcattt atactcaaac ctacttctcc
     4681 ggtttcaaaa taataaaaat taactttgtg ggagagcgca atgtttcgaa attcttcttg
     4741 agaaacattt tcgggcaaaa ctattttcaa agtatcgaaa aaaagtttat tcaactgacg
     4801 ataaccgtaa gttggcagca ttgcattgat ataagctgct atggcatgaa tgtttttcgc
     4861 tatatttcta attccctccg gcccgtgaaa aacggtataa aaacccgcca tcgaagccaa
     4921 aagagcttgt gcggtacaaa tgttagaagt tgccttttcg cgtttgatgt gctgctcacg
     4981 ggtttgaagt gccatacgca aagccggttt cccgttttta tccttgctaa tgccaatgat
     5041 gcgacccggc atatcgcgct taaattcatc gcgtgtagca aaataagccg ccgaaggacc
     5101 tccaaacccc ataggaattc cccaacgttg agtagaacca aaaacaatat cggcgcccca
     5161 ttctcccggt ggaacaagta aagccaaact taaaatgtcg gcagccacag caactttaca
     5221 accagctgta tgtgcttgtg atacaaatga aaaataatgc tctacattcc catccgcatt
     5281 aggatattga agaatcgctc caaaatgttt ttctgaaaat tgtacggatt tataatcccc
     5341 aatttcgagt tgtattccct gtgcttgtgt ccgcgtttgt aaaacagcca aagttgaagt
     5401 aaaaatattt tcatcgacaa aaagcaaatt tgctccattt ttcatttgtt ccctcgaacg
     5461 caaattatac atcatggtag ctgcttctgc tgcggcagtt gcttcatcga gcaaagaagc
     5521 atttgccaat ggaagaccgg taaattcaca aacggcggtc tgaaaattca gcaaagcttc
     5581 caatcttcct tgagaaattt cggcttgata gggagtgtag gaagtgtacc atgaaggatt
     5641 ttcaaaaaca ttccgtaaaa ttacggccgg tgtacaggtg tcataccatc ctctgccaat
     5701 ataggaagta aaaattttat ttttttctgc aatttcagaa atatgctgag catactgatt
     5761 ttcagtcaga gcagaaggga gattcaattt ttcgttcaaa cgaatggaga gaggaatagt
     5821 ttgagtgata agttcatcta aactttcagc ccctacaact tccaacattt cttgaatttc
     5881 atcagacgaa acgccaatgt gtcgaaaaga gagaaaatct ttcatttgat tatatattac
     5941 tgatttttag ttaattataa aaatttgaat gatcgaatat taaaaaataa agcggctaaa
     6001 atttcaagtt gtaaagttac agcaaaaaaa tcaggaaaga aaattcaatt ttttaacaaa
     6061 atattgtttg agtccactcc gaaaagtttt tcaaagagaa ttagttttta aagacattgg
     6121 aagtacttga ccatttgtag agaaggtttg tgccgttggt gcttatgaca gtgagaaaaa
     6181 tcaagaattt gcaacgacaa acaacaaaga acttatttca agtggtatac agggagccaa
     6241 aggaagatat gaactttcag ttgattcaca aaatcctgac aatttaatag taaccaatac
     6301 ccagacagga gaaattataa aagcacaaaa ataaaaacaa aaagaagagt ggtaatctac
     6361 aatggcgtat caaaacagag catggcaact atcgttattt taaccttgag caggtgcaaa
     6421 tttttcttgc ttcttttcaa aaaaaaatct cattttttat cgtttttgaa aaaaattagg
     6481 ttttaaaaaa aaggactcaa tacttaattt aggtcagctt tacaaataga caataattca
     6541 atacgtttta ttaaatttgc aaatcaaact tcgattataa gtaaaaaaac atggattctt
     6601 agaatgattt taaacaactt ttcaaacttt tggcagacaa aaaggtactg agttgttaat
     6661 tttctaagca atattcacga tgataatgat tgatacatta aatttatttg gtaaaaccaa
     6721 aatagaagtt ccaaaaactg aaggaattaa atatgctggt tcaaaactaa aaattcttcc
     6781 atatattttt aatatgattt ctggcttaga tgtagaaaat atattagatg gattcagtgg
     6841 aacaacaaga gtatctcaag cacttgccaa aacgggtttt aaaactactt caaacgatat
     6901 atctatatgg tcagcaactt ttgcaaaagc atatttgcta aataatagaa agcctgaata
     6961 ctatttgcct attattgagt atttaaattc attaaaaggt tatgtaggtt ggttctctga
     7021 gtattatgga ggcactgaaa ataataaaga aaaaaggcct tttcaactta aaaatacaat
     7081 gaaactggat gcaattagag atgagattga tagaatgaat ttagatgaaa tagaaaagtc
     7141 tgtagctcta acaagtttaa ttctcgctct tgattcagta gacaacacat taggccatta
     7201 tgctgcatat ttagcagaat ggtctcctag atcatatcgt gatttaaaat taaaattacc
     7261 gttattattt aaaaataatc agcaaaatga agtaataaaa ggagatattt ttgaaacagt
     7321 aaaaaatcat aaatttgatc ttgcttattt tgatcctcct tatggttcaa ataatggaaa
     7381 aatgccacca agcagagtta gatacaattc atattatcat atctggacaa ctgttatatt
     7441 gaatgacaag ccaaaacttt ttggaaaggt taatagaaga ctggattcaa aggatgaggt
     7501 agcagcgtca gttttcgaag aatttagaaa agatgaacaa ggtcatttta ttgcaatgaa
     7561 tgcaataaaa gaattgattc attgtacaaa ctctaaatat attcttttat cgtatagttc
     7621 gggaggtaga gctacaaaag aagaactttt tgatataata aattctgaag gtaaaatgat
     7681 tcaagcaatt gagataaatt ataagaaaaa tgttatgggt caaatgagat ggacaaatga
     7741 atggataaat agtgatgata aatatatgga atacctcttt ttaatggaaa aatgttgatt
     7801 ccactcactt caaatttatt gttacttaca ttgagaaaaa ttttttgaaa gaattgaaag
     7861 cctaaaatag attaaaaaca aatcatagaa atgattacat cagaaccaat tattcatgta
     7921 gggatattaa gttcccggga aatagcattt attctaaatg gcatttttat tatttccgat
     7981 ggaaaaaatt tttccgggaa gcaaaaagcc gaatggaaaa atgggaaaat actttttgat
     8041 ggcgaaactt ttgatgaatt gaattttcaa ccgcatgacg aatcgtcaac atttgattta
     8101 cttgacgtag tgatcgggat tcattttcat tgggaaagga aagaaaatca gcgatttaaa
     8161 ggagctttga aaattattgt ggaaaatgag caattaacag ttgtcaacga aataaaggcc
     8221 gaagattatc tgctcagtgt aatttccagt gaaatgagtg ctacctcctc acttgaactg
     8281 ctgaaagccc atgccgttgt ttccagaagc tggctgtttt atcctctgca aaaagacaaa
     8341 aaagagcttt ccggtcggga atcggttgaa gaaaacacgg aaaatcgaca tatcaaatgg
     8401 tacgaaagag gcgaacacga acattttgat gtgtgtgccg acgatcattg tcagcgttat
     8461 caaggaatta caagagcaac ttcggatgcg gttcgacatg cagtagaagc cacgcgtggc
     8521 gaagtgctga tgtttgagca acaaatatgt gatgctcgtt tttccaaatg ctgtggcgga
     8581 gttaccgaaa cttttgacaa ttgctgggcg cccgaagagc atccttattt atccagtatt
     8641 gtggacaatg atttattacc tcaaagtttt gatttggatt taacgatgga agaaaatgcc
     8701 caaaaatgga ttcgtgaatt tccgacagct ttttgcaata cgcaggacaa aaatgttctc
     8761 acacaggtgt tgaataatta cgatcaggaa accacagatt tttatcgctg gaaagttgtt
     8821 tacacccagg aagaacttgc cgaaattttg cgacgtcgtt caggatatga tttcggagat
     8881 attttagact tgattccttt gaaacgaggt aaatcgggcc gaattgtgga gttaaaaatt
     8941 gtgggaacca aacgaacgat gatagtaggt aaagagttgg aaattagaaa atggctgtcc
     9001 gatagccatt tgtatagctc ggcttttgta gttgacaagg aaaattattc ccaacaaatt
     9061 ccaggaaaat ttattttgac cggtgcgggt tggggacatg gcgtaggact ttgtcagatt
     9121 ggtgctgccg ttatgggcga aaaaggttat gcctacgaac gtattttgct gcattatttt
     9181 cgaggcgctg agttgaaaag gatttatgaa tgaagaatag tatttttagc attttcttga
     9241 ttccatttca tcagaatata atttattttt taatggtctg atggaaatca catgtctaaa
     9301 tgttatattc attcgtgttt gtgtatgtaa tagatgtggg tttcttaatt gaaaataata
     9361 aataaatttt gagtccatat ttttaatatt ttgtataaat ttacttaatt gattaataat
     9421 caattgttta aaaaaataac ataatctgta taaatggttg attgtaagta taatttcaat
     9481 attgatgtca gagaatattc gagaataaaa aatttttcac ttttttagtt tactctattt
     9541 caatagtttg ataaagtaat atactttcat tcattggtca ttttcagttt ttcaaactat
     9601 ctttgcaagc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaattataaa
     9721 gaagtggttg tttttaatcc tttttcgcac aatgctgttt atcaacgtta ttatttggtt
     9781 cgggatactg atgtgtctgt gccggcggat ggcttgaaaa tcaaaattcc tgttcgttcg
     9841 ttggcagcaa catctgttac ccactttgaa tttttgaatt tgcttcatgt aattgacgct
     9901 gtaacgggtg tctgttcttc aaaaattatc tacagtccgc aaattaatga gcgtctcaac
     9961 aaaggcttga tagtcgattt gggagatgca ttcaatatca atctcgagaa aacggttcag
    10021 ttgcatcccg atttggtcat ggtgagcggc tacaatcaaa acgacccttt tgcacagcgg
    10081 ttgcttcagg ccggaatttt tccggtttac aacaacgaat ggatggaaaa atcgctgttg
    10141 gggcgcgcag aatggataaa attcgtggcg gttttttttg acaaggaaaa aattgccgac
    10201 agtctttatc tggaaattga aaaggaatat caaaatactg ccaacagggt aaaagggata
    10261 aaatttcgtc cgcaaataat gtccggttct aattttcgtg gcacttggta catgcccgga
    10321 ggacgaaatt ttatggcaca gcttttttcg gatgccagtg gaaattattt ttatgccaac
    10381 gatacaaccg taggaagttt accgctcagc tttgagaagg tactgaaaaa tttctccgag
    10441 tccgatgttt ggctaaactg caattatgct tcattgtccg aattgttgca ggccgatccg
    10501 agacatgctc ttttccgtcc cgtaaagttg caacaggtgt ataatttcaa taaacgcatg
    10561 ttgccatccg gcgccaacga tttttgggaa agtgcggtag ctcatcccga tttgcttttg
    10621 aaagatgtga tttttatttt gcatcccgaa ttgctgcccg gttacgaacc attttacact
    10681 caaaaattaa atccgtaagg tttttatgta ttcgagaaac catttgcttt tttttgcttt
    10741 ttcgttgctg tttatttttt tgtttctggc cgatttggca tggggaagtg ttgccattcc
    10801 ggcaaaagaa atttattcgg tgctcctcgg gaaacaatct tcctcaattt atacggaaat
    10861 aattgttaat tttcgtttgc caaaagccat tactgcgata cttgccggcg cttcgctttc
    10921 ggtagccggt cttatgatgc agacgctttt tcggaaccct ttggccgatc cgtatatttt
    10981 gggcgtcagt tcgggtgcaa gtttaggtac ggcgctggtg gtgatggctt ccggtttttt
    11041 accgttttcc tttgttggca atggttgggc actgataatt tcggctattg ttggcgctgc
    11101 attggtgctg ttgattgttc tttttgtttc ctttcgcgtc aacgatattg tttcgctgct
    11161 cattgtcggt attatgtttg gaaccattgc agctgctttg gtgtcgatta ttcagaattt
    11221 cagcaatccc gatgccataa agctgttttt gatgtggaca ttcggaagtt taagcgccgt
    11281 tacgtggaat tatttgcaaa ttttgttgcc tgttgttttg gtaggcatag gtattgcttt
    11341 ttttctgcaa aaaaggctcg acggactttt attgggcgaa aattatgcac ggggtttggg
    11401 tattcacatt tcacaaaccc gatcgctgat tattctggct accggattac tggcaggagg
    11461 aatcactgct tttactgggc ccattgcatt cgtaggggtg gcagttccgc atatagcacg
    11521 aggaattttt caaacttcat cacacaaaat tttattgccg gcatgtatgc tggtcggtgc
    11581 agcattgttg atgatttgcg atatcattac tcaaattccc gattatacgt tgccggtgaa
    11641 taccatcagt gccttatttg gtgcgccggt aattatttgg atcattttga gaagaaaata
    11701 agtcatgaag aaattataag tcaataatat gtcggtttta tccacacaac atttatcgat
    11761 tggttaccgg agaaaaggaa cggctaacgt aattcaacat gacttgaatt taaaactgga
    11821 atcagggaaa ctggtaggtt tgattggccc gaacggaagt ggaaaaacta cgcttttacg
    11881 aactattgca ggattgttga aacccatcga cggaaatatt ttgatagatg acaaagagtt
    11941 tcttttgctt tcgcaacgtg aaaaagcatt gaatgtttct tttgtactta ccgaaaaagt
    12001 agatattccc aatgccgatg ttttcgatat ggtatccttt gggcgttttc cctataataa
    12061 ttggcgggga aatctttcca agcaggacag aacgaaagtt tttcaggcgc tgcaaatggt
    12121 caggatggaa aaaatgatgc atcgatatat caatgaactt tccgacggag aacgtcaaag
    12181 agtcatgata gccaaagcgt tggctcagga tacgcccatt attttactcg atgaaccaac
    12241 tgcccatctc gatgttccca atcgtgtggg aattatgttg ctgctccata aattggctca
    12301 taaaaccggt aaaatgattc tgctttcgac acacgaactg gatttggcgt tacaggctgc
    12361 cgacctgatt tggctgatga gcaaaaacgg aggaattgaa tgcggagtcc ccgaagattt
    12421 ggtttttagc gggagtttca atcgaacttt tgccagcgat tattattttt tcaatgaagc
    12481 caatggtaac ttttccctga attatccttt gacaggaaag gtgagcgtat cgggcaacaa
    12541 aatgcgtata tattggactt tgcgggcttt ggcacgtgca ggatatgaag cggtggaaaa
    12601 tgctccgttg aaaatagaag ttaccgacag tgcatggata ctgaatcaaa aggaaatttt
    12661 ttcggtggcc gaaatgttgg atgaattgaa taatttgaat ggcaacactt cattataatt
    12721 tattttgact tcttagtcag tttaacgtaa atattcacta ccgatattcc tatatatcga
    12781 ttaactgcgc ctgtttgtcc caactttaca aggtagttct ttactataaa gataaccata
    12841 atcatcaatt ccttcaacta aaataattga tttttaattt attaagtgaa tttttcaccg
    12901 aagtattatg aatgaagttg tcgatattaa attctttttc tttagattga ctaatatcta
    12961 tgcttagaca catattttta aatggggacc cataaatttg gttaattgga ttcttgaaaa
    13021 aacaggatga gaaataggtt atttgtcggt tttttttctt tattttgtat tgaattacat
    13081 taccaaatta atttttaaaa ggctatgagc aaaaaattga cgcgttcgag caatcaaatg
    13141 atagccggag tttgtgccgg aatagcagaa tattttggtt ggaatgtaac tttagtaaga
    13201 gttatttatg ttttgctttc catcttttct gcaggattcc ccggtactat ggtttacatt
    13261 attctttgga ttgtcatgcc tcgccccgag aaattcgatt aaataaccga aagaacaaaa
    13321 gtcgttgtga aaacgacttg gaaacaaacc tataatttac acttttttaa acgttatgaa
    13381 ttttaagctc gaatcgccct atcagcctac cggtgatcag ccggaagcca ttcagcaact
    13441 ggtagaggga atcaatgccg gtattccgta tcaaactttg ttgggtgtta ccggatcggg
    13501 gaaaaccttt accatagcca atgttattga gcaggttcag cgtccgacgc tggtactgag
    13561 tcacaacaaa acgcttgctg cccagttgta cagcgaattc aaatcttttt tcccacacaa
    13621 tgccgtagaa tattttgttt cctattacga ttattatcag cctgaagcgt atttgccatt
    13681 gaccgatacg tatatcgaaa aagatttgtc catcaatcag gaaatcgaaa aactgagact
    13741 ctcggccacc tcttcgcttc tttcgggacg cagggatgtt gtggtggtat catcggtttc
    13801 gtgtctgtat ggcattggta atcccgacga tttttcgaaa aatgtaattg aaattcgaaa
    13861 aggacagcat ttggtgagga atgctttttt aagaaatttg gtcaacagcc tttatacccg
    13921 taacgaaatt gaactgaatc gtggaaattt tcgcgtaaag ggcgacactg tggatatttt
    13981 tcttgcctat accgatttta ttttgcgtat cgttttttgg ggagacgaaa ttgatgagat
    14041 actgacagtt gatccagtca attacgacat tattgattct cttgatgcct ataaaatata
    14101 tccggccagc atttttgtaa ccacaaagga acgaattgct gtggccataa atgaaatcga
    14161 aaaagatttg cagttgcagg tggattattt tcgctccatt ggaaaagatc tcgaagccaa
    14221 aagaatttat gaaagagtac gttacgatat cgaaatgata aaagaacttg gttattgttc
    14281 gggaatcgaa aactattcgc gatatttcga cggtcgtccg ccgggaagcc gtcctttctg
    14341 tctgttggat tattttccga aagattttct gttggtcatt gatgaaagtc atgttaccat
    14401 tcctcaaata agagccatgt acgggggcga tgcttcacgt aaacagaatt tggtcgattt
    14461 cggattccga ttgccggctg ccaaagacaa tcgtcccttg cgttttgaag aatttgaaca
    14521 gcttacacct caaactattt atgtgagtgc aacccctgct gattatgagt tagtaaaatc
    14581 ggaaggtatt gtggttgatc agcttatacg tcctaccgga ctgctcgatc ccgttattga
    14641 ggttcgtccg agtttaaatc agattgacga tttgctggaa gaaattgccc agcgaaccga
    14701 aaacaacgag cgggtgctgg taactaccct taccaaaaga atggctgaag aactgaccaa
    14761 ttatctatca aaaatgggag tgaaatgcaa ttatattcat tctgatgtcg aaacgctgga
    14821 tcgggttcaa atcatggaag atttgcgcag aggagtgtat gatgtgttgg tcggtgtcaa
    14881 cctgttgcgc gagggattgg atttaccgga agtttcactg gtggctattt tggatgccga
    14941 taaggaagga tttttgcgtt ctcatcgttc gttgacgcaa acggcagggc gagctgcacg
    15001 aaatttgaat gggaaagtca tcatgtatgc cgatcagatc accgaaagca tggaaaagac
    15061 cataaatgaa accaatcgcc gcagagagaa gcaacttcga tacaatgaag cgcatcatat
    15121 tacgccaact gctattgtga aaggcgaaag aaatccgctg tcgaaagcag aaccatacgc
    15181 gtatgtggaa ccggaaaagt cgaccgatat tgcagccgat ccggtggtgc gatatatgtc
    15241 cgtgccggca cttgaaaaag ccatagcaaa aaccagaaaa gcgatgcagg aagctgccaa
    15301 gaaactcgaa tttctggaag cggctcaact cagggacgaa ttgatgaaac tggaagaatt
    15361 gttgaaaaag aaaatgttcg aggcgaagaa ttgaaaagaa aatgttgctt taaaagtttc
    15421 aaaaaatcaa tttcataagt cgaaacaaaa ggacaattca ttcaaaaaac accaaacaag
    15481 ccctaaaaat tgttgaatta ttgcattaaa tttagttttc tgatgataga aaaattatct
    15541 ttgtatcgct aattcaccag aaaacctata atatgctgga aatcattccg aaaggtattc
    15601 taattggttt gtgtatttct gttccggtag gtcccattgg tatgctggtt attcagcgta
    15661 ccttgaatag aggaagaaaa tacggagtgg ccaccggttt gggtgcgagc atgtccgatt
    15721 tattttatac catcatcact ttattctttt tgagttttgt gatcagtttt attgaggctc
    15781 atcgttttgt catagaattg ataggaagtt tggttgtgat ttgttttgga tatttcatat
    15841 ataaaaataa tccgtcagta caacccaaac caaacgagac agttcagcac aatgtatacc
    15901 gcgactttat cagttctttc gtattgactt tctctaatcc cctcattttg tttattttga
    15961 ttgcattatt tgccagaatt ggatttattg actcagaaac atcttttttt caaaccatta
    16021 ttggtatcat atctatttta attggtgcta ctatttggtg gttaactctt acttttattg
    16081 tcagccattt caggtcaaaa attggaatgc accagttgaa agccataaat cgaataacag
    16141 gtggactgat tgctttgatt ggatgtattg gacttgtttc cagtttgata taaaaattca
    16201 cccttctttc ttcgcagaaa aaagggtgta cttcactttg ggtgactcat ttaacccaga
    16261 atccgcaata gggaaaaagt tttaattttg taggaaagtc taatgcgtag caatagaaaa
    16321 aaagacgtgt atgaactttg gtatcttctt tacaaacaaa gagataacac catgtggtgg
    16381 aatgattttt ttgtaacaaa tgttggaaaa accacgtaga atcgt
//
LOCUS       DAOSIB010000048        16413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_3706_length_16413_cov_10.571525, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000048 DAOSIB010000000
VERSION     DAOSIB010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16413
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3706_length_16413_cov_10.571525"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..823
                     /locus_tag="PLD44_05590"
     CDS             248..823
                     /locus_tag="PLD44_05590"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOF98841.1"
                     /translation="MEKLLQEEIRKAVEVLRQGGTILYPTDTVWGIGCDATSAEAVKK
                     VYEIKRRADSKALLVLVDSASRILSYVDEVPDMAWQLIEITDKPLTIIYPEAKNLAAN
                     LIAADRSIGIRVTKELFSKNLCEQFRKPIVSTSANISGNPTPSNFSQIDEAIKSAVDY
                     VVNWRQDDISEPKPSSIIKLSKGNVIQIIRE"
     gene            complement(1337..1936)
                     /locus_tag="PLD44_05595"
     CDS             complement(1337..1936)
                     /locus_tag="PLD44_05595"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOF98842.1"
                     /translation="MSSEIKLTITGLVYNQNIVGTYGLVLSEESGNRRFSVMIGEPEA
                     QSIALKLNNKKLPRPLTHDLIKTILDVFEAKLVKVLISDMINDVFYSELHILKDEKIL
                     VVDARTSDAVALAVRCNSPIYIKSDILDIVGVVVEENEIEETTDNEENIEELSDQELD
                     NLSLNELEELLSMAVNEEKYELAVHIRDAIQRKKTKEKF"
     gene            complement(2161..2793)
                     /locus_tag="PLD44_05600"
     CDS             complement(2161..2793)
                     /locus_tag="PLD44_05600"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOF98843.1"
                     /translation="MSVLVGKQAPVFSSKAVVNGNEIVDNFSLAQFKGEKYVVFFFYP
                     ADFTFVCPTELIAFQEKANEFEARNTVVVAASTDSAFSHWKWLQTPQNEGGIKGVTYP
                     IVADHSLTISKAYDVLAGDVDYDEEGNEIFVGAPEAYRGLFLIDKDGVVRHQVVNDMP
                     IGRNVDEVLRVIDALQFTEQYGEVCPANWKKGDKAMKATQEGVADYLSHK"
     gene            complement(2943..>3290)
                     /gene="ispF"
                     /locus_tag="PLD44_05605"
     CDS             complement(2943..>3290)
                     /gene="ispF"
                     /locus_tag="PLD44_05605"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008125054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOF98844.1"
                     /translation="LCDALLGAAGLRDIGYHFPDTADEFENIDSKILLAKTMNLLNEK
                     GFSVGNVDCTVCAEEPKLNAYIPQMQTCLANLMQLDETDVSIKATTSEKLGFVGRKEG
                     IAAYAVVLIYRPS"
     assembly_gap    3291..3390
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3391..4524)
                     /gene="porV"
                     /locus_tag="PLD44_05610"
     CDS             complement(3391..4524)
                     /gene="porV"
                     /locus_tag="PLD44_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HOF98845.1"
                     /translation="MKKSFLIFSSFVLITFCVYAQTDTNNPVITAVPSLTIAPDARAG
                     GMGDVGAATTPDVNSQYWNPAKYSFMESEVGVSLSYTPWLSKLVSDINLVYAAGYWKF
                     DELQSVSASLRYFSLGDISLMDEMGYPQGSAHPNEYAFDIAYSRLLSTKFSASVALRY
                     IRSDLNNGVNLSGGTEMYPGNAFAADVAGYYRTPITMATGDANLALGFNISNIGSKIS
                     YDENETSNFIPTNLRIGGSFDYPMDAYNRFSFSADVNKLLVPTQNDMTIEEYSDISSI
                     SGIFKSFNDAPGGFNEELKEVMWSVGAEYAYNNQFFVRAGYFNESQEKGNRKYFTAGA
                     GFKLNVFELDAGYVIATSQSNPLDQTLRFSLSFDLNGLRNLAR"
     gene            complement(4618..8352)
                     /gene="porU"
                     /locus_tag="PLD44_05615"
     CDS             complement(4618..8352)
                     /gene="porU"
                     /locus_tag="PLD44_05615"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOF98846.1"
                     /translation="MAQNTITTSHKLIWKGIEKWYADSSSVQVISFRDAHYPYENRLP
                     YFNYRIPAENYSYKAVLTNTVFEAVNTEEAAILSDKAVYSSEPVLETRYLVDRGSSFF
                     DVQMLPFVFQNGKFLKLISFDLSISKTALPQQAKNAFTHSFASSSVLAQGKFVKVRLA
                     DSGIYKLTYEDLVAMGINPSNVRVFGYGGGILDQNFSQTKPDDLPEVPIWVEKGSDGI
                     FNAGDYILFYAQGVVRWSYDKNKSMFVHKNNSYSQYGYYFITSDAGVGRKIESNSIEL
                     PADVTIYQVDEFTDYQLYEKDIISLTKSGKEFYGETFSNTLSYSFVFNLPNVVKSNTL
                     KLSVDVAASSLENSSFSLNLNGSQNKTLSVTKNSGDNYEIAKSASSVYTFTPDNETLT
                     FNITYNKSTSTSTGYLNYLEVNARRKLVMQGAAMRFQNVDYLGKSSYNNYTLSNVNGN
                     VQIWDITDPQNIYRVATTTLPDNKLSFVASGNELHQYIAIDPTAGSSFPKPEIVGVVA
                     NQNLHALQPSDLVIITHPNFLSQAETLAQAHREKDGLSVVVATTEQVYNEFSSGTPDA
                     TSYRWFVKMLYDRAIETNNTADLPKYLLLFGRGSYDNRGIVPNSGDNLILTYQAENSL
                     VLTSSYVTDDYFGFMDDSEGTQVPADLMDVAVGRFPVTTVQQAIDVVNKTINYIDNKG
                     KGIWKNQICFLADDGDGALHMRQADSIAVAVAKKVPAFQINKIYLDAFVQEVNASGQS
                     YPLARSRFHNLLRSGMFLLDYTGHAGYAGWTNENILTTADVKSLSNKNLPVWMAATCD
                     FLQFDVKPVSAGEYVILNPLGGGIGLLSAARPVYASQNFYINKYFVENLFKKNGDYPR
                     VGDAVRLAKNSIGTETNKLSYIYLGDPAVRLNYPDQYNVVTSKINDNVALGKDTLKAL
                     STASVSGFVADQNGNKVSDFNGTVEIVVYDKVEKITTLNNEGDGSMTYYDRPNTLFAG
                     KVKVINGEFTFTFLLPKDIKYNYGSGRINYYAYDDTNNYEAQGYFENFLIGGSSTNLP
                     NDSIGPQIDMYLNSPEFVSGGQVNETPLFVANISDENGINTVGSGIGHDLLLTVDNDP
                     NKTYILNDYYTAVENSYQQGTVSYKLSTLQEGKHTLTFRAFDLLNNSTTKTIEFEVVK
                     GLSPVIFSVYNYPNPVREQTKFVIQHDRPETLLTTTVEIFDLSGRKIWMFTQSSTDEV
                     TWDLKTSNGEKVQKGIYLYRVSIRTENSSLTSKTNKIIVF"
     gene            complement(8507..9118)
                     /locus_tag="PLD44_05620"
     CDS             complement(8507..9118)
                     /locus_tag="PLD44_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOF98847.1"
                     /translation="MKIFAIGQNYPEHNKELKNDNPVEPVVFMKPDTALLKNNKPFYI
                     PDFSNELHYETELIIRFNRIGKNIQSKFAHRYFDEIGLGIDFTARDLQRKLKAEGKPW
                     EICKAFDNSAVVGDFLPASDFRDIQDIQFHLDINGITVQKGDSKDMIFSVSELIAYVS
                     RFFTLKIGDILFTGTPAGVGCVHPGDRLEGYIGNRKMFNFLVK"
     gene            complement(9151..9804)
                     /locus_tag="PLD44_05625"
     CDS             complement(9151..9804)
                     /locus_tag="PLD44_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOF98848.1"
                     /translation="MSDIKFNDELRVPEPTLRRLPWYLAYAKLMMEKGETYLSSTQIS
                     NNIAVDASIVAKDLSYVQISGKTRVGYETHELIIVLERFLGFTESHKAFMFGVGNLGG
                     ALMHDNGLEQFGLKIVAGFDVKYELAGTSINRIPIHHIEKFPELQRQTGVIIGILTVP
                     VDKAQEVSELMIEGGIRAIWNFTPFRIRVPDNVVVQNTSIYAHLAVMFNRLNAIVNK"
     gene            10009..12765
                     /gene="leuS"
                     /locus_tag="PLD44_05630"
     CDS             10009..12765
                     /gene="leuS"
                     /locus_tag="PLD44_05630"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOF98849.1"
                     /translation="MEYNFREIERKWQTYWKDNQIYKTVENPSKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRYKRLKGFNVLHPMGFDAFGLPAEQYAIQTGQHPAITTKN
                     NIARYRQQLDKIGFSYDWDREIRTCDPDYYHWTQWAFIQMFHHYYDNNLQKAQPISRL
                     VEHFEKKGTENLHASGTRELSFTAADWKALSEKQQQEILLNYRIAYLADTKVNWCPAL
                     GTVLANDEVSEGVSIRGGYPVEQRIMRQWCLRVSAYAQRLLDGLETIDWTDSLKETQR
                     NWIGRSEGAEIRFSIKGREETFEVFTTRADTIFGVTFMVLAPESELVKICSTPQQAAE
                     VEKYLENTKKRTERERLADRRITGVFSGSYAIHPVTGKEIPIWISDYVLAGYGTGAIM
                     AVPAHDSRDYAFAKHFNLPIIPLIEGCDVSEESFDAKEGIMINSGFLNGLTVKEAIEA
                     TKKYITEKGIGRVKVNYRLRDAIFSRQRYWGEPIPIYYKDGVPYTLSEEELPLELPEV
                     DKFLPTEKGEPPLARAKNWKTKEGYPLEVCTMPGFAGSSAYYLRFMDPKNDQALVSPE
                     ANQYWRNVDLYLGGTEHATGHLIYSRFWNKFLYDLGIVCEEEPFKKLINQGMIQGRSN
                     FVYRIKDTNTFVSYHLKDQYETTPIHVDVNMVSNDVLDLEKFKNWNPEYKNAEFILED
                     GKYICGWAIEKMSKSMFNVVNPDDIVENYGADTLRLYEMFLGPLEQSKPWDTNGIDGV
                     HRFIKKLWNLFYKNDELSVSEENPTAEELKVLHKTIKKISYDIENFSYNTAVSAFMIC
                     VNELFTLKCNKKAILEPLLILLAPFAPHISEELYHTLGNSDTVFNASFPQYDEEFLKE
                     NTVFYPVSFNGKVRFTLEMPTDASKEEVEKTALSHPQTLKYMEGKTPKKIIIVPGKII
                     NIVF"
     gene            12807..13694
                     /locus_tag="PLD44_05635"
     CDS             12807..13694
                     /locus_tag="PLD44_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOF98850.1"
                     /translation="MAKFNPFTKKNWIFIRQYILITIGLFMYAAAWKGFLLPHQITGG
                     GVTGIGAIVYYATGVPIFITYFVINALLIAVALRIIGFQFSLRTIYGVAMITLFFAVL
                     PQAEPGTFVAPNDNFMACLLGGLISGTGLGIVFLNNGSSGGTDIIAKIVNKYYNITLG
                     RILLYCDVLIICSSYFFEFGTIEKIVYGLTNMAISNLAIDMVINGVRQSVQFFIFSKE
                     YEQIANRINTEVHRGVTILDGTGWYSQQPVKVITVIARKNESSMIFRIVKEIDPDAFV
                     SQSSVIAVYGKGFDVIKSK"
     gene            13716..15188
                     /locus_tag="PLD44_05640"
     CDS             13716..15188
                     /locus_tag="PLD44_05640"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOF98851.1"
                     /translation="MGDNLKQKTLSAFTWTTIDRFGQQLVQLIIGIILARLLSPGDYG
                     LMGMVMIFAALSYVLVESGFGQALIRKTEANETDYNSVFYFNIGIALVLYALLYFLSP
                     AIANFFKQPELVSVNRITFLAIIFNAFYLVPYSKSAKILDYKTIAKVNIISVSLSGLL
                     GLTLAFFHYGIWVLVIQHVSYHFFRMIFFYVFVRWKPKLIFSFRVIRSFWKFSIHLLN
                     TSILNIIFNYLFVIILGKFYKKSEVGYYMQGNKLSETFNFTFQSILLGSTFSLFSHIQ
                     HDDARFRRIFGELSNKISIITFPIILCLIAAGKPLIEIVWSAKFLPSVPYFRLLCLAS
                     LFLPLYTLNISALNARGQSRITFRIEMIKKILILIAIFITFSKGIIPMLWGYAVANIA
                     AYFLSIFYIKSELKHGLKQQILGFIQSLGWGLVLGLMVASLSLLIKPSYFLLPVQIFL
                     AAAVYLLIIKIFYKELYDKGMDFLKNRLLSLKQGLHQNKF"
     gene            complement(15173..16210)
                     /locus_tag="PLD44_05645"
     CDS             complement(15173..16210)
                     /locus_tag="PLD44_05645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOF98852.1"
                     /translation="MLKKRSGKKIYDTSYTHFETEGIDYEYLSIKRNIFNVFKERLQR
                     EKTFLKYNELIFNQIYNNVKRENFQLIHAHGMYEPPAGLIAKHFSEKLKIPYIITCHG
                     SDVNYKMPLTPQLYIDTFEQAAKVLFVSNAILNKAKSYGYSGKNAVIIPNGVNIDLFK
                     MLDKEKIKQELHFIRKVVGFVGNLLPVKRADKLPEIFEEINQNFETDFLIIGEGRLKN
                     KIEKNCRKKNLNITFTGKLPHEKISYYLNAMDVLILPSRNEGFPCVVLEAQACGVPVV
                     GSNNGGIPEAIGEGGIVVNEGENFEKRFAEGVIKILKNPIDKNFLRNRATEYSWNNIT
                     KMEFDIYQNLF"
BASE COUNT         5120 a         3172 c         2958 g         5063 t
ORIGIN      
        1 gttcaagctg aattattttc ataatgagct gaaatacaaa tatatgaatt ttcaaaaaca
       61 acatttttaa aaactttcgt acggcagtga taaaaattaa ccaatttgca gaaattgtta
      121 ggaaatctca gtttcggtag aagtaaaact tgggtggaat aatttatcct tttttgaagt
      181 aaggcgtatt tttgttattt ttgcttggag ttttcattga aggtttggat taaactatca
      241 ttacaaaatg gaaaaattat tacaggaaga aatcagaaaa gctgttgaag tgttgaggca
      301 aggaggtact atactttatc ccaccgatac agtgtgggga attggttgcg atgccaccag
      361 cgcagaggcc gttaaaaaag tttacgaaat aaagcggcga gccgatagca aagccttatt
      421 agttttggtt gattccgcct cgagaatact ttcttatgtg gacgaggtgc ccgatatggc
      481 ttggcagttg atcgaaatta ccgataaacc tctcaccatt atttatcccg aagccaaaaa
      541 tctggctgcc aatttgattg ctgctgatcg ttcaataggc atcagggtaa caaaggaatt
      601 gttttcaaaa aacttgtgtg aacaattcag aaaacccatt gtttccacgt ctgccaatat
      661 cagcgggaac cccacgccgt caaatttttc gcaaatagat gaagccataa aatcggctgt
      721 cgattatgtg gtaaattggc gacaggatga catttccgaa cccaaacctt cttccattat
      781 caagttaagt aaaggaaacg taatccaaat tatcagggaa taaaatcgca aaaagttttt
      841 ttgcattcgt actttttctg ttgtttatac taaatacatt aaggttacat tttttatttt
      901 gatgaaagaa ctttgatact atgacttttt cctgtaagtt tttatagcca acgaatatcg
      961 aaaaaattct tcatatgata aagaatagtt gaataaccag gtctaatttg ttgtaaaaag
     1021 ttcacaagtt gtacattaat ttcatcagaa tataatttat tttttataag gtctgatgga
     1081 acccacatgt ctgaatatta tattcattcg tgtttgtgta tgcaatacat gtggatttca
     1141 taatatcatt cttgattaat tttacaataa catttgctat gcatactaac agaaaaaaat
     1201 tataattaaa ctttccgctg atattgaccc attgcttttg gtttatagta ttttaatttt
     1261 caattcaata attcgataaa gtgaagcaat tccgtagtca tcaacttatg gttgaaacaa
     1321 atttattttg agaaaatcaa aacttttctt ttgttttttt gcgttggata gcatctcgga
     1381 tgtgtacagc cagttcatat ttttcttcgt ttacagccat agaaagtaat tcttccaatt
     1441 cgttgagcga aaggttgtcc aattcctgat cggataactc ttcgatattt tcttcgttgt
     1501 cggttgtttc ttctatttcg ttttcttcca caacaacacc tacaatatcc agaatatccg
     1561 atttgatgta tattggactg ttacatctaa ccgctaatgc cacagcatct gaagttcgtg
     1621 catcaaccac caatattttc tcatctttga gaatatgcaa ttcagagtaa aaaacatcat
     1681 ttatcatgtc gctaatcaac actttcacca gttttgcttc aaaaacgtct aaaatagttt
     1741 ttatcagatc gtgagtgagt ggacgtggaa gttttttatt gttgagcttc aatgcgatag
     1801 attgcgcttc gggttctcca atcatcacgg aaaatcgtcg attgcccgat tcttcactaa
     1861 gaaccaagcc gtaagtaccc acaatgtttt gattataaac caaccctgtt atcgttaatt
     1921 ttatttctga actcatacag actatttagt tgcaattagt tgacaaaatg acttttatca
     1981 ttaaactcga attgttattc tttctttatg acaaaggtac acaatttttt taaaaacgat
     2041 aaccaaaaat gaatacatag tttttctgtc tttaatgttg catcaaaaat aggttaaaag
     2101 tatattaaaa aagccgcttg agtaatttaa gcggcttttt ataagttgat cactcacctt
     2161 ttatttgtgc gacaagtaat cggcaacgcc ttcctgagtg gctttcatgg ctttatcgcc
     2221 ttttttccag ttggccggac aaacttctcc atattgttcg gtaaactgca acgcatcaat
     2281 tacacgcagc acttcatcca catttcttcc gattggcatg tcgtttacta cctgatgacg
     2341 cactactccg tccttgtcga taagaaaaag tcctctgtat gcttccggcg ctcccacaaa
     2401 aatttcgtta ccttcttcat cgtaatccac atctccggcc aacacatcat aagctttgga
     2461 aatagtcaac gaatgatcgg ccactatcgg ataagttacg cccttgatac cgccttcgtt
     2521 ttgaggggtt tgcagccatt tccaatgaga aaaagctgaa tcggttgacg ctgccacaac
     2581 cactgtgttg cgagcttcaa attcgttggc tttttcctga aaagcaatta actctgtagg
     2641 acatacaaaa gtaaaatcgg caggataaaa gaaaaatacc acatatttct ctcctttgaa
     2701 ctgagcaaga gaaaaattat ctacaatttc gtttccgttt accacagcct tgctgctaaa
     2761 aacgggtgct tgttttccaa ctaatactga cataattata aatttatagt tgttttaatt
     2821 tttactgata caaaagtaat cattatttta taaaatgatt cacaaaattc aatagatttt
     2881 atcaattgtt ctataagaat tttgaatgct tgataggtag aggggaaaca agggaaaaaa
     2941 aatcacgatg gacgataaat cagtactact gcataagccg caattccttc ttttctacct
     3001 acaaaaccca atttttcgga tgtggtggct ttaatggaaa cgtccgtttc gtcaagctgc
     3061 atcagatttg ccagacacgt ctgcatttgt ggaatatagg catttaactt tggttcttct
     3121 gcacaaaccg tacaatccac gttaccaaca gaaaagcctt tttcattaag caaattcata
     3181 gttttagcaa gcaatatttt gctgtcgata ttttcaaatt catcagccgt atcgggaaaa
     3241 tgatagccta tatcgcgcaa accggcggct cccaaaagtg catcgcataa nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttaccttgcc aaatttctta atccgttcaa
     3421 gtcgaacgac aaggagaaac gcaaggtctg atccagcgga ttcgactgcg aagtagcaat
     3481 cacataaccg gcatcaagtt caaaaacgtt taatttgaat ccagcaccgg cagtaaaata
     3541 cttgcgattt cccttttcct gactttcgtt aaaataacct gcacgaacaa agaactgatt
     3601 attgtaggca tattctgcac ccaccgacca cattacctct ttcagttctt cgttgaatcc
     3661 gcccggagca tcgttaaacg atttgaaaat accactgatc gaagaaatat cgctgtactc
     3721 ttcaatggtc atatcgtttt gggtaggtac cagcagttta ttgacatccg cactgaacga
     3781 aaaacggttg taagcgtcca taggataatc aaatgaaccg ccaattcgca agttggtcgg
     3841 aataaaatta ctggtctcgt tctcatcata cgaaatttta gaaccaatat tggaaatatt
     3901 aaaacccaat gccaaattag catcgcccgt tgccatagta atgggtgtac ggtaataacc
     3961 tgccacatcg gctgcaaagg catttcccgg atacatctct gttcctccgg ataaattaac
     4021 tccattgttc aaatcggaac gaatatagcg caaagctacc gacgctgaaa atttggtgga
     4081 gagcaaacgg gaataggcta tatcgaaagc atattcgttg gggtgggcag agccctgagg
     4141 ataacccatt tcgtccatca acgaaatgtc tcccaacgaa aaatatcgca gggatgcact
     4201 taccgactgc agttcatcaa atttccaata acctgcggca taaaccaaat taatgtcgct
     4261 taccagcttg ctcagccaag gcgtgtaaga tagagaaacg ccaacttcgc tttccataaa
     4321 agaatatttg gccggattcc aatactgaga attgacatca ggtgttgtag ctgctcccac
     4381 atcgcccata cctccagccc gcgcatcggg tgcaatggtt aaagacggta cggcggtaat
     4441 tacaggattg ttggtatcag tctgagcata aacacaaaaa gtaatcaaaa caaaagaact
     4501 gaaaatcaaa aaacttttct tcattattct tatttattac attaaaaatc aaaaaattaa
     4561 aaaaacagat atttcaaatt agcaaaaata ttaacgaaca atttgaattt tattgcctca
     4621 gaaaacaata attttgtttg ttttcgacgt caacgaactg ttttcggtac gaatacttac
     4681 ccgataaaga taaatacctt tctgaacttt ttcgccgttt gaagttttca gatcccaagt
     4741 tacttcatcg gttgaagatt gagtaaacat ccaaattttt cgacccgata aatcgaaaat
     4801 ttcaaccgta gtggttagaa gtgtttcggg acgatcgtgc tgaatgacaa atttagtctg
     4861 ttctctgaca ggattcggat aattataaac cgaaaaaatg acaggagaca aacctttgac
     4921 tacttcaaat tctattgttt tggttgtaga attattgagc aaatcaaatg ctctgaaagt
     4981 taaagtatgt tttccttcct ggagcgtaga taatttataa gaaactgttc cttgctgata
     5041 actgttttct acagccgtat aataatcatt caaaatgtat gtcttattcg ggtcgttgtc
     5101 caccgtcagc agcaaatcat gaccaatacc gctgccgacc gtatttatac cgttttcgtc
     5161 agaaatattg gcaacaaaaa gcggggtttc attcacttgt ccgcctgata caaattcagg
     5221 agaattgaga tacatgtcga tttgggggcc aatactgtcg tttggtaaat tcgtacttga
     5281 gccaccaatt aaaaaattct caaagtaacc ctgcgcttca tagttgttgg tgtcgtcgta
     5341 agcatagtag tttattcgtc ccgaaccata attatacttg atatcttttg gaagaagaaa
     5401 agtaaaagta aactcaccgt tgatcacctt cacttttccg gcaaacaagg tattgggacg
     5461 atcgtaataa gtcatcgacc catcgccttc attatttaaa gttgtaattt tctcaacttt
     5521 atcataaaca acaatttcca ctgtaccatt gaagtcggaa accttgttcc cgttctgatc
     5581 agcaacaaag cccgaaacag aagcagtaga aagagctttc agcgtatctt ttcccaaagc
     5641 tacgttatca ttgattttag aagtaaccac gttgtattga tccggataat tcaatctgac
     5701 agccgggtcg cccaaataaa tataagaaag tttattggtt tcggtgccga tggaattttt
     5761 tgctaaacgg acagcatctc ctacacgcgg ataatctcca tttttcttaa agaggttttc
     5821 gacaaaatac ttatttatat agaaattttg cgacgcataa accgggcgag cagccgataa
     5881 aagtccaatt ccaccgccaa gaggatttaa aataacatat tctcccgccg aaactggctt
     5941 aacatcaaat tgaagaaaat cgcaagtggc agccatccaa acgggcaaat ttttattcga
     6001 caaagatttt acatcggctg tagtgaggat gttttcgttt gtccaacccg cataaccggc
     6061 atgacctgtg taatccagca aaaacatacc ggaacgcaac aaattgtgaa agcgacttct
     6121 tgccaaagga taactttgcc cgctggcatt gacttcctgg acaaaagcat cgagataaat
     6181 tttgttgatt tgaaaagccg gaactttttt tgccactgct actgcaatgc tgtcagcttg
     6241 ccgcatgtga agagctccgt cgccatcatc ggccaaaaaa caaatttgat ttttccaaat
     6301 tccttttccc ttgttatcga tataattaat tgttttattc accacatcaa tagcctgctg
     6361 tacagtagta acaggaaaac ggccgacagc tacatccatc aaatcagcag gaacttgcgt
     6421 tccttcgctg tcgtccataa aaccgaaata atcatccgta acataggaag aagtgagtac
     6481 caacgagttt tccgcctgat aggtcaatat caaattatca cctgagttgg gaacaatacc
     6541 tctgttgtcg taagagccac gaccaaacag caggagatat ttcggcaaat cggcagtatt
     6601 gtttgtttcg atagctcgat catacaacat ttttacaaac cagcgatagg acgtagcatc
     6661 cggtgtaccg gacgaaaatt cgttataaac ctgttcggtg gttgctacta caaccgaaag
     6721 tccatctttc tctctatgag cctgcgccaa tgtttcggcc tgtgaaagaa aattgggatg
     6781 ggtaataata accaaatcag aaggctgcaa tgcatgaaga ttctggttgg caaccacccc
     6841 aacaatttct ggcttgggaa aggaactacc ggccgtagga tcaatagcaa tgtactgatg
     6901 gagttcgttg cctgaagcta caaaagaaag tttattgtca ggaagtgttg tggttgcaac
     6961 acgataaata ttttgagggt ccgtaatatc ccaaatttgc acattaccgt tgacattact
     7021 caaagtatag ttgttgtaag aagattttcc taaataatca acattttgaa aacgcatggc
     7081 agcaccctgc ataactaatt ttcgtcgggc atttacctcc agataattca aatagccggt
     7141 ggaagttgaa gttgacttgt tgtaagtaat attgaaagtt aatgtctcgt tatcgggagt
     7201 gaaagtataa accgaagaag cagattttgc gatttcataa ttatctcccg aattttttgt
     7261 aaccgagagc gttttattct gagaaccatt caaatttaag ctaaaagacg aattttccag
     7321 cgaagaagca gcaacatcaa cagaaagttt taatgtattt gatttaacta cattgggcaa
     7381 attaaaaaca aaactgtagg aaagcgtgtt gctgaaagtt tcaccgtaaa attcctttcc
     7441 cgatttggta agactgatga tatccttctc gtagagctga tagtctgtaa attcatcaac
     7501 ctgataaatc gttacatcgg caggaagttc aatgctattt gactcgattt ttctgcccac
     7561 tcctgcatcg gaggtgataa aataataacc atattgtgaa tacgaattat ttttatggac
     7621 aaacattgat ttgtttttat cgtacgacca gcgcaccaca ccctgcgcat aaaataagat
     7681 gtaatcgccg gcattgaaaa ttccatcact tcctttttcc acccaaattg gaacttcggg
     7741 caaatcgtca ggtttggttt gagaaaagtt ttgatctaaa atgccaccac cataaccaaa
     7801 gaccctgaca ttggatggat taatacccat cgcaaccaaa tcttcataag taagtttgta
     7861 aattcctgaa tcagccagtc tgactttaac aaattttcct tgtgccagca ccgaagaaga
     7921 agcaaaactg tgtgtgaaag cattttttgc ctgttgtgga agagcagttt tggaaattga
     7981 taaatcgaaa gagattaatt tgagaaattt gccgttttga aaaacaaaag gcaacatctg
     8041 aacatcaaaa aagctgcttc ctctatcgac aagataacga gtctccagca ccggttcgga
     8101 agaataaaca gctttatcgc ttaaaattgc tgcctcttca gtgttgacag cttcaaaaac
     8161 agtgttggtc aaaaccgctt tgtacgaata attttcggcc ggaattctgt aattgaaata
     8221 aggcaaacga ttttcgtaag gataatgcgc atcccgaaac gaaatgacct gaacggaaga
     8281 tgaatcagcg taccattttt caattccttt ccaaatcagt ttatgagaag ttgtgatagt
     8341 attttgagcc atacaccaac cactgacaga aaggaaaaaa gaaaaaaata aaaatttaat
     8401 tttcataaaa gcgccctcaa gggagaaata aaatttaata caaaaaaaca gataatttaa
     8461 aaacgttcaa aacaaaaagt tattatttca gggattagtt gctcaattat ttaacaagaa
     8521 aattgaacat ttttcgattg ccgatatagc cttccaaacg atcgccggga tgaacacaac
     8581 caacaccggc aggtgttccg gtaaataaaa tatcaccaat ttttaaagta aagaaacgac
     8641 taacgtaagc aataagttca ctgaccgaaa aaatcatatc tttggaatca cccttttgta
     8701 cagttattcc attgatatcc aaatgaaatt gaatatcctg aatatcccta aaatctgaag
     8761 caggaagaaa atcgcctaca acagccgaat tgtcaaacgc cttgcaaatt tcccaaggtt
     8821 ttccttcggc tttgagtttt ctctgcaaat cgcgggcagt aaaatcaatt cccaaaccaa
     8881 tttcatcgaa ataacgatgg gcaaatttag actgaatatt tttacctatg cggttaaaac
     8941 gtataatcaa ttccgtttcg taatgcaatt cattggaaaa atcgggaata taaaaaggtt
     9001 tgttgttctt caacagcgct gtgtcgggtt tcataaacac aacaggttca acaggattat
     9061 catttttcaa ttctttattg tgttcgggat agttttgccc gatagcaaaa attttcattt
     9121 tttcctttaa ctaatgaatg ataaaaaaaa ttatttgtta acaatggcat tcaagcgatt
     9181 gaacattacc gccaaatgag cataaataga agtattttgc accaccacat tgtcgggcac
     9241 tcttattcta aaaggagtaa agttccaaat tgcacgaatt cctccttcaa tcattaattc
     9301 cgacacttcc tgtgccttgt ccacagggac agtcaaaatg ccaatgatta cacctgtttg
     9361 tcgctgtagt tctggaaatt tttcaatatg atgaatggga atgcggttga tactagtacc
     9421 tgccaattcg tattttacat caaaacctgc cactattttt aacccaaact gttccagccc
     9481 gttgtcgtgc atcaaagcac ctcccaaatt tccgactcca aacataaaag ctttgtgcga
     9541 ttccgtaaaa ccaagaaagc gttccaaaac gataatcaat tcatgggttt cataaccgac
     9601 tcttgttttt cccgaaattt gaacataaga taaatccttg gcaacaatgg aagcatctac
     9661 agcaatgttg ttcgatattt gagtagaaga caaataggtt tcaccctttt ccatcataag
     9721 ttttgcataa gccaaatacc atggcagtcg tctcaatgtt ggttcgggaa ctcttaattc
     9781 atcattgaat ttaatatcgg acatctttct ggagattagt aatcacctgc aaaaataatc
     9841 aaattattga aatatttata cttatacttt ataaaaatga gcgaaagatg aaacggaaaa
     9901 agaagaagct ttcttttatt ttggcaaaat gattaacttt gcaaatttaa tttgcgaata
     9961 ctgtaaaaga gaaaagataa ggttgaattt attaaatttc agtgatacat ggagtacaat
    10021 ttcagagaaa tagagcgaaa atggcaaaca tattggaaag acaatcagat ttataaaacc
    10081 gttgaaaacc caagcaaacc caaatattat gtattggata tgtttcccta tccttcggga
    10141 gcagggctgc atgtcggcca tccgctcggt tacattgcat cggatattta cagccgttac
    10201 aaaagactga aaggttttaa tgtactccat ccaatgggct ttgatgcttt cggactgccg
    10261 gcagaacaat atgccattca aacaggccaa catccggcca ttacgaccaa aaacaacata
    10321 gctcgctacc gccaacaact tgataaaatc ggattcagtt acgattggga tagagaaatc
    10381 agaacctgcg atcccgatta ttaccactgg acacaatggg ccttcattca aatgtttcat
    10441 cattattatg acaacaattt gcagaaagct caacccatta gtcgtcttgt tgaacatttt
    10501 gagaaaaaag gtactgaaaa tcttcatgct tcgggcactc gtgaactgtc gtttacggct
    10561 gccgactgga aagcgttgag cgaaaaacaa caacaggaaa ttttgttaaa ctatcgcatt
    10621 gcctatttgg ccgacacaaa agtgaactgg tgtccggctt tgggtacggt gctggccaac
    10681 gacgaagtga gcgaaggcgt ttccattcgc ggtggatatc ctgtagagca acgtatcatg
    10741 aggcaatggt gtctgcgcgt ttcggcttat gcccaacgtt tgctggatgg tttggaaact
    10801 atcgactgga ccgactccct gaaagaaaca caaaggaatt ggataggaag aagtgaaggt
    10861 gccgaaatcc gtttttccat aaaaggcaga gaagaaactt ttgaagtatt taccacacgt
    10921 gcggatacca tatttggcgt tacatttatg gtacttgctc ctgaaagcga attggtaaaa
    10981 atatgttcca ctccccaaca ggcagctgaa gttgaaaaat acctcgagaa caccaaaaag
    11041 cgtacagaac gtgaacgctt ggcagatcgg cgtattacgg gagttttttc cggttcttat
    11101 gccattcatc ctgttaccgg gaaagaaatt cccatttgga tttccgatta tgtgcttgcc
    11161 ggttacggaa caggggctat tatggctgtt ccggcgcacg acagcagaga ctacgctttt
    11221 gccaaacatt tcaatttgcc catcattccg cttatcgaag ggtgtgatgt cagtgaagaa
    11281 agtttcgatg ccaaagaagg tatcatgatc aactcgggat ttctgaatgg actgaccgta
    11341 aaagaagcca ttgaagccac aaaaaaatat attacggaaa aaggaatagg cagggtaaaa
    11401 gtcaattatc gtctacgcga cgctattttc agccgccaac gttactgggg cgaacccatt
    11461 cccatttatt acaaagacgg agtgccctac acacttagcg aagaagaact accgcttgaa
    11521 ttgcccgaag tggacaaatt tttgcctacc gaaaaaggtg aaccgccatt ggctcgcgct
    11581 aaaaactgga aaaccaaaga aggctatccg cttgaagtct gtactatgcc cggttttgca
    11641 ggttcttcag cttattattt acgatttatg gatccgaaaa acgaccaggc acttgtaagc
    11701 cctgaagcca accaatactg gagaaacgtg gatctttatc tcggtggaac cgaacatgca
    11761 acgggtcatt tgatttacag ccgtttttgg aacaaatttt tgtacgattt gggtatcgtt
    11821 tgcgaagaag aaccatttaa aaaactcatt aatcaaggca tgattcaggg aagaagcaac
    11881 tttgtgtacc gaataaaaga cacgaatacc tttgtttcct atcatttaaa agaccaatac
    11941 gaaacaactc cgattcatgt tgatgtcaac atggtttcca acgatgttct cgatctggaa
    12001 aaatttaaaa actggaatcc cgaatacaaa aatgccgaat ttattcttga agatggaaag
    12061 tacatttgtg gttgggctat agaaaaaatg tcgaaatcga tgttcaatgt tgtcaatccg
    12121 gatgatattg tggaaaatta cggcgcagac acccttcggt tgtacgaaat gtttttggga
    12181 ccgcttgaac agtcaaagcc atgggatacc aacggcatag atggcgtcca tcgttttatt
    12241 aaaaaattgt ggaatctttt ctacaaaaac gatgaactat cagtatcgga agaaaatccg
    12301 actgcggaag aattaaaagt tttgcacaaa accattaaaa aaatcagcta cgacatagaa
    12361 aatttttcct acaacacagc tgtttcggct ttcatgattt gtgtgaacga attgtttacc
    12421 ctgaagtgca ataaaaaagc catacttgaa cctttattga tactgctggc acctttcgct
    12481 cctcacattt cggaagaact ttaccacaca ctgggcaaca gcgacaccgt ttttaatgcc
    12541 tcttttcctc aatatgatga ggaatttctg aaagaaaata cggtatttta ccccgtttcg
    12601 tttaacggga aagtgagatt tacgttggaa atgcctactg acgcaagtaa ggaagaagtt
    12661 gaaaaaactg ctttatcgca tccgcaaacc ttgaagtaca tggaaggaaa aactccgaaa
    12721 aaaataatta ttgttccggg taaaattatc aatattgttt tctgacaaga aataattcag
    12781 aaaaaacatt caatcccaaa taaataatgg caaaattcaa tcctttcaca aaaaaaaact
    12841 ggatatttat ccgacaatac atcctgataa ctatcggttt atttatgtat gctgcagcat
    12901 ggaaaggttt tttgctgccg catcaaatta ccggtggagg tgttacaggt ataggagcaa
    12961 tagtttatta tgcaacgggg gttccgattt ttatcactta ttttgttatt aatgccctgc
    13021 tcatagctgt agcactgcgt attatcggat ttcagttcag cttgcgcacc atttacgggg
    13081 ttgccatgat taccctgttt tttgccgtgc ttccacaggc agaaccggga acttttgttg
    13141 ctccaaacga taattttatg gcatgtttgc tgggaggttt gatttcggga acgggattgg
    13201 gtatcgtttt tctgaataac ggcagttccg gcggaacaga tattatcgca aaaatagtca
    13261 acaaatacta caacattaca ttgggcagga tactgcttta ttgcgatgtg ctgattattt
    13321 gctcttccta tttttttgaa tttggaacca ttgaaaaaat agtgtacggc ttgaccaaca
    13381 tggctatttc aaatttggcc atcgatatgg ttatcaatgg agtacgacag tctgttcaat
    13441 tttttatttt ttcaaaagaa tacgaacaaa ttgccaaccg tattaatacg gaagttcatc
    13501 ggggcgtaac cattctggat gggacgggat ggtattcgca acagccggta aaagttatta
    13561 ccgtcattgc ccgaaaaaat gaatcttcca tgattttcag aattgtaaaa gaaattgatc
    13621 ccgatgcctt tgtatcccaa tcatccgtta ttgccgttta cggaaaaggc ttcgatgtga
    13681 tcaagagcaa ataattttta ttgtaccatt accacatggg cgataacctg aaacaaaaaa
    13741 ccctcagtgc ttttacttgg acgaccattg accgcttcgg tcaacagctg gttcagctga
    13801 ttattggcat catactggcc aggcttcttt ctccgggcga ttatggatta atgggcatgg
    13861 tcatgatttt tgcggcactt tcgtatgtgc tcgttgaaag tggatttgga caggccttga
    13921 tcagaaaaac cgaagccaat gaaacggact acaacagtgt attttatttc aacatcggta
    13981 ttgctttggt tttgtatgca cttttgtatt ttctttcgcc ggctattgcc aattttttta
    14041 agcaacccga acttgtttcg gtgaatagaa ttacttttct ggccattatt ttcaatgctt
    14101 tttatctggt tccctattca aaatcggcaa aaattcttga ttataaaacc attgccaaag
    14161 tcaatattat ttctgtttcg ctcagcggtt tgctgggcct tacacttgct ttttttcatt
    14221 atggcatctg ggtgctggta attcaacatg tttcctatca ttttttcaga atgatatttt
    14281 tttatgtctt tgtccgatgg aagccaaaat tgattttttc attccgggtt attcgtagtt
    14341 tctggaaatt tagcattcat ttactgaata cttccatttt aaatattatt ttcaattacc
    14401 tgtttgttat tattttgggg aaattttaca aaaaatcgga agttggatac tacatgcaag
    14461 gaaacaaatt aagtgaaact ttcaatttta cttttcaatc cattttattg ggcagtacat
    14521 tttccctttt ctctcatatt cagcatgatg atgcccgttt caggagaatt tttggtgagc
    14581 tgtcgaacaa aatctccatt attacttttc ccataatttt gtgtctgatt gctgccggaa
    14641 aaccattgat agaaattgtt tggtcggcaa aatttcttcc gtctgtccct tatttccggc
    14701 ttctttgtct ggcttccttg tttttgccac tttacacact caatatcagt gcattgaatg
    14761 caagaggtca gtcgcgaatc actttcagaa tagaaatgat aaaaaaaata cttatcttga
    14821 ttgccatttt tattacgttc agcaaaggaa taattcccat gctctggggc tacgcagttg
    14881 caaatattgc agcctatttt ctttcgatat tctacataaa atcagaattg aaacatggtt
    14941 tgaaacaaca aatactggga tttattcaga gtctgggttg gggacttgtt ttaggcttaa
    15001 tggttgcttc cttaagtttg ctgataaaac cctcctattt tttgctgccc gttcaaatct
    15061 ttcttgcagc agctgtttat ctgttaatta tcaaaatttt ttacaaagaa ctgtatgata
    15121 aaggtatgga ttttctaaaa aatcgactgc tcagtttaaa acaagggcta catcagaata
    15181 aattttgata tatatcaaat tccattttag taatattatt ccatgagtat tcagtagctc
    15241 tgttccttaa aaagttttta tctatcggat tttttaatat ttttatcact ccctcggcaa
    15301 atcgtttttc aaagttttca ccttcattca caactattcc accttctcca attgcttcag
    15361 gaattcctcc attgttgctc ccaactacgg gcactccgca agcctgagct tccaacacta
    15421 cgcaaggaaa accttcattt ctgctgggta atatcaacac atccattgca ttcagataat
    15481 aagaaatttt ttcatgaggt aattttccag taaaagtgat attcaaattt ttttttctac
    15541 aatttttctc aattttgttt ttcaatcgac cttcgccaat aatcaaaaaa tcagtttcaa
    15601 aattttgatt tatttcctca aatatttctg gcaatttatc cgctcttttt accggcagaa
    15661 gatttcctac aaatccaacc actttcctta tgaagtgaag ttcttgttta attttttctt
    15721 tatccagcat tttaaataag tctatattaa ccccattagg aataataacg gcattttttc
    15781 cagaataacc ataacttttg gctttattta aaatggcatt gcttacaaat agaactttag
    15841 ctgcttgttc aaaggtatct atataaagtt gaggtgtcaa aggcatttta taattgacat
    15901 cgctaccatg acaggtaatg atataaggaa tttttagttt ttctgaaaaa tgttttgcta
    15961 ttaatcctgc cggtggttcg tacatcccgt gggcatgaat caactgaaaa ttttctcttt
    16021 taacattatt atatatttga ttaaaaatca actcattata ttttaaaaaa gttttttctc
    16081 tttgtaatct ttctttgaaa acgttaaaaa tattcctttt aatggaaaga tattcataat
    16141 caattccttc tgtttcaaaa tgggtatagg aagtatcgta aatttttttt ccacttcttt
    16201 tttttagcat cttgaccaaa acagtgtcat cgggaataat gccataaacc ttgaaagaaa
    16261 gatttttttc tttcatttcc tttaatcgat tggttaggaa agagcctcct tgtaaatcat
    16321 cgaaaaaagg aaatagattg gtaaaatata atactttctt attcaatata ttgggttttt
    16381 gaaactaatt tctcaaaaaa ttagagtttt gct
//
LOCUS       DAOSIB010000049        15798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_3887_length_15798_cov_12.866417, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000049 DAOSIB010000000
VERSION     DAOSIB010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15798
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3887_length_15798_cov_12.866417"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..895
                     /locus_tag="PLD44_05650"
     CDS             8..895
                     /locus_tag="PLD44_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOF98853.1"
                     /translation="MENINAFREVTPLSSEDCFFIIDRIKSKMTYPVHYHPEYELNFI
                     ENGKGIIRIVGDSLEEIDDLELCLIGNEQLEHGWVEPKNREPELMHEITIQFHPDLFL
                     ESLLNKLQFQSIAVLLENAKKGVVFSREAIYSIKNRLKLLSEHESNFEAVIELFSILN
                     LLSHDNNYRILCNSSFVKNDDVSESRRVQKAINYIKMHYTEKLSLNDVANQVNMTTTS
                     FSRFMKSRTGKNFIEYLNDLRLGSAARSLVNTNKTISEICYECGYNNLSNFNRLFKQR
                     KGYTPKEFREKYKKMRKLI"
     gene            1438..2391
                     /locus_tag="PLD44_05655"
     CDS             1438..2391
                     /locus_tag="PLD44_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HOF98854.1"
                     /translation="MKTFIRVQDLGNLQEALQEAFEVKKNRFGYQHLGKNKTLLMVFF
                     NSSLRTRLSTQKAGMNLGMNTIVLDINQGAWKLETERGVVMDGDKTEHILEAIPVMGN
                     YCDLIGVRSFAQFENKAYDYQETILQQFIQYSGRPVFSMESATVHPLQAFADLITIEE
                     YRKKPHPKVVMSWAPHPKALPQAVPNSFADFMNEADVEFVITHPKGYELAPEFVRNAR
                     IEYDQMKAFEGADFVYAKNWAAYQDPNYGKIICTDRSWTVSSRQMAVTNNAYFMHCLP
                     VRRNMIVTDEVIESPQSLVIPEAANREISAEVVIKRMLESL"
     gene            2455..2979
                     /locus_tag="PLD44_05660"
     CDS             2455..2979
                     /locus_tag="PLD44_05660"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOF98855.1"
                     /translation="MDVEQVIASIRNIPDFPKKGVLFKDITTAMKDAEVLRFISDELY
                     EYYKDKGITKVVGLESRGFMLGSILAYKLHAGFVLIRKAGKLPAATYRVDYELEYGVD
                     SLEIHKDAIEKDDVVLLHDDLLATGGSANAALTLLDNFKPKAIYVSFLIELKDLKGRD
                     RLKGVQDVHSLIQF"
     gene            3051..3863
                     /gene="argB"
                     /locus_tag="PLD44_05665"
     CDS             3051..3863
                     /gene="argB"
                     /locus_tag="PLD44_05665"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOF98856.1"
                     /translation="MNSDKSPNIDMFQKKEKLTIIKVGGKIVEESDSLISLLNDFSKI
                     DGYKVLVHGGGRSATALATRLGLETKMIEGRRVTDAEMLKVVTMIYAGWVNKQIVAGL
                     QALGLNALGLTGADLNYMRSEKRSPYPIDYGFVGDVKEVNVSLLTDLIRKEVVPVLAP
                     LTHDKCGNLLNTNADTIAAEAAKAFSSFFEVTLIYCFEKKGVLMNEKDEDSVISSLTP
                     ELYEKFVSQKVIQGGMIPKMENSFNALKSGVSKVIITRADAILTHSGTTITL"
     gene            3925..4983
                     /locus_tag="PLD44_05670"
     CDS             3925..4983
                     /locus_tag="PLD44_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HOF98857.1"
                     /translation="MTAELANEAIQLLRQMIAIPSFSKEEKEIADFLEQYLTERGYVV
                     FRHLNNIWLQSPGFDVSRPTILLNSHLDTVKPGSSWIRNPFEASLKNGKLFGLGSNDA
                     GASLVSLLQVFFYLTQRPQPYNLIFAATAEEEISGKNGIEIMLQEFPKVDFAIVGEPT
                     GMNMAVAERGLMVIDATVTGKSGHAAREEGENAIYKALDAINWFKNYRFAKVSPLLGP
                     VKMTVTQINGGIQHNMIPDKCTFVVDVRSNELYSNEEILEIIHENVDCRIEPRSVRLC
                     STATQPDHPIIKRAQQMNLTLYGSPTLSDQALMPFPSVKIGPGDSARSHTADEYVLVE
                     EIEKGIEVYIQLLDGLQI"
     gene            complement(5005..5850)
                     /locus_tag="PLD44_05675"
     CDS             complement(5005..5850)
                     /locus_tag="PLD44_05675"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOF98858.1"
                     /translation="MFKKIYIEITNSCNFHCSFCFTTSQPPRFMPVKDFHTIVRKIKP
                     FTSYIYLHVLGEPFLHPHIEDILNVAEQEGMNVNITTNGSLLNNQKELLLNHSIRQLN
                     ISLHDAEENIPSTNWESFFENIFNIAMEISAKTYVNLRLWNIDNLVSGKFNSLCKEKI
                     AEKFQLSPDELTQPEKGNSIKLANHIFLQQSPKFQWPTENGEPLFENKSCYALRDHIA
                     ILAGGEVVPCCLDAGGSLKLGNIFTEELSDILMCERATKMKQGFRQHKITEPFCKTCG
                     FSVAL"
     gene            complement(5863..6819)
                     /locus_tag="PLD44_05680"
     CDS             complement(5863..6819)
                     /locus_tag="PLD44_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOF98859.1"
                     /translation="MDFVKRSKEIFEQEISELQKLAEKIGPEINRAVELIYNCKGKLV
                     ILGIGKTGIIGHKIASSLASTGTQTIFINAAEAMHGDLGMIDRNDIALLISNSGSSPE
                     IVNLITPLKKIGCTIIAMTGNPKSPLAREASLIINTGIEREACPLGLAPTTSTTATLV
                     MGDALTICLMERRNFKAENYALYHPGGALGRQLLARVKDQMNHDIPKVYTHTLFKDVI
                     YEVSNKRLGMTMVYDDNEKAVGIITDGDIRRAIQRFDDLKNLTAQEFMTHGFKKIAQN
                     EMLSEALEIMDENNITNLAVMENDSSSTVVGILSIHDIIDFK"
     gene            complement(6832..7356)
                     /locus_tag="PLD44_05685"
     CDS             complement(6832..7356)
                     /locus_tag="PLD44_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HOF98860.1"
                     /translation="MVNQPVEKVQLPQKEKQKIKLLLSDIDGVMTDGGLYYSEFGDQS
                     KKFHVRDGMGLKILQSKGIKVGIVTSEDNKIAEMRYNKLQLDYLYKGRKNGGKLAAAL
                     EICEKEGISLQQTAYIGDDVNCYELLCSVGLAACPADAMELIKSVPGIIQMKAKGGQG
                     CLREFVEYILKNYC"
     gene            complement(7387..8199)
                     /gene="kdsA"
                     /locus_tag="PLD44_05690"
                     /pseudo
     CDS             complement(7387..8199)
                     /gene="kdsA"
                     /locus_tag="PLD44_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
     gene            complement(8236..9558)
                     /locus_tag="PLD44_05695"
     CDS             complement(8236..9558)
                     /locus_tag="PLD44_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98861.1"
                     /translation="MALFGNKFFRKITPTGKFERSENERVQSLQRYNEIARSAELAEY
                     QQLSKVISSPKFQENKKILKNRKYKDTEEYRLSSEYKKLQNSHSLQQYFKTLESKELN
                     DFLKFKETPDYLDLGNRNKVKASEELKKFKQFEHSKEYKNFVRYHDSFVVKEYEELKE
                     KVNSPEFKKSNDFWANPDRWHTTKEYALQRRHHQLRKNPDIIYYENENPERFGIFRSL
                     ELTFEENFDWNTLDSSRWKYGFHYKSPQLIGDHSFANEKQANNSGKNVIVSDGRLKIF
                     TKEEKVTARAWDIQKGFIMKEFDYTSDVIQTSDSFRQKYGMFRAKLRCSGKIHHAFWL
                     GGDEMLPHINIFHFDGKYINVGNASKNEMDGTKIKGIHPSEFYIYSLVWTEKELIWMI
                     NNYEVYRTVRNIPDEELYLALNSFIPEKEKGDKGVLEVDWIKVYQFKQ"
     gene            complement(9572..10120)
                     /locus_tag="PLD44_05700"
     CDS             complement(9572..10120)
                     /locus_tag="PLD44_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOF98862.1"
                     /translation="MNVAVFCSSSNQVQPVYKQAAFLLGQFIAESGFTLVYGGSTGGT
                     MDDIALGATEKKGKIIGVIAQPIIRMHRQSTLPTELIVVEDLSERKNKMKQLADVFVA
                     LPGSYGTFDEMMDVIASGVVGEHHKPIAVVNEQHFFDDFIRQINRMKIEQFLPEMENY
                     RPVFVDNINQCKRFLQSLSIRN"
     gene            complement(10217..12175)
                     /gene="gyrB"
                     /locus_tag="PLD44_05705"
     CDS             complement(10217..12175)
                     /gene="gyrB"
                     /locus_tag="PLD44_05705"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOF98863.1"
                     /translation="MITEEKLNAIAEYDASSIQVLEGLEAVRKRPAMYIGDIGIKGLH
                     HLVYEVVDNSIDEAMAGHCDDIQVTIHPDNSITVTDNGRGIPVDFHEKEGKSALEVVM
                     TVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSSFMRVEVARNGKIYEQEYERGIPKFP
                     VREIGTTSTTGTRTTFIPDDTIFTEVVYKYDIIADRLRELAFLNAGIRLTLVDERTVE
                     EDGSFRKEVFYSKEGLKEFVRYIDEAREKLIDDIIHIRNEKNGIPVEIAMTYNTSYNE
                     NIHSYVNNINTIEGGTHLAGFRRGLTRTLKKYAEESKMLDKVKIEISGDDFREGLTAV
                     ISIKVAEPQFEGQTKTKLGNNEVMGAVDMAVGEALSAYLEENPKAARMIVEKVILAAT
                     ARNAARKAREMVQRKSPLSGGGLPGKLADCVERDPAKCELFLVEGDSAGGTAKQGRSR
                     QFQAILPLRGKILNVEKAMQHKVLESEEIRNIYTALGVTIGTEEDSKALNISKLRYHK
                     IIIMTDADVDGSHIATLIMTFFFRHMKELIEQGYLYIATPPLYLCKKGKIEEYCWTEQ
                     QRQAFIEKYGNGQEGSIHVQRYKGLGEMNETQLWETTMNPEKRTLRQVTIENAASADH
                     VFSMLMGDDVLPRRQFIEQNATYANIDA"
     gene            12507..13304
                     /locus_tag="PLD44_05710"
     CDS             12507..13304
                     /locus_tag="PLD44_05710"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005803970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOF98864.1"
                     /translation="MEIELNQLCLSVCEIARSAGKMIADERKRFDDSKVEYKGLHDLV
                     SYVDKNSEKLIVSQLEKLLPEAGFLAEEGTTSKEGERYNWIIDPLDGTTNFVQGVPVF
                     AVSIGLHEANEMVLGVVYEIGRDECFYAWKGGGAFLNGRAIHVSERDNIQDALLATGF
                     PYSEFSRLNEYMEFLKWAVTNSRGVRRLGSAATDLAYVACGRFEAFWEYNLKAWDVAA
                     GSIIVKEAGGTVTDFSGKNNFLFGKEMVATNQKLKQLFLDKLKEMMF"
     gene            13366..13914
                     /locus_tag="PLD44_05715"
     CDS             13366..13914
                     /locus_tag="PLD44_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HOF98865.1"
                     /translation="MIINERNTRRERLMQAANAMMTAARTAPKGKGYDIIEIAIITDE
                     TIVELSNAMFEYSQQSGLKFITRDAENILKSEVILLIGTRQQNQNLNCGYCGFDTCAE
                     KNSQPDIPCALNTVDVGIAIGSACAVAADLKVDTRVMFSVGRVAKEIGLLPGCSSIFG
                     IPLSCSSKNPFFDRVSKTPAPK"
     gene            13927..15147
                     /locus_tag="PLD44_05720"
     CDS             13927..15147
                     /locus_tag="PLD44_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF98866.1"
                     /translation="MTQSKEKLWTASFISICVANFMLFFAFYLLLPILPLYLIENFQA
                     SKSLVGIILSSYTIAALLIRPMAGFLLDMFQRRPIYLLAYFLFSLCFIGYPLANFLNF
                     FLSLRIVHGLCFGMVSTASNSFVIDIMPSSRRGEGLGYFGIANNLAMSIGPMISLFLH
                     DYSSYQMIFFTSIASSLIGFVVVLTVKAKKTTIPTIKKYKEAIVLDRFFLIKGTHAGI
                     CLTLMGIPYGMLTTFIAIYGKEIGMYSGMGIFFTLMAIGLIFSRFFAGRMVDRGHLLK
                     VITVGTIVCTVSIFVLPALKDLFLLQQNFSMILFYVVAVFLGLGYGMLFPAYNTLFVN
                     LAPHNRRATASSTFLTTWDVGIGLGLIIGGKLGDTHGGLSLSFLTGGLIAFSSLVYFI
                     KITAPHYKKNTIIT"
     gene            complement(15327..>15798)
                     /locus_tag="PLD44_05725"
     CDS             complement(15327..>15798)
                     /locus_tag="PLD44_05725"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOF98867.1"
                     /translation="FYDFEAKKINGENISMQQFKGKTVLVVNTASKCGLTPQFEGLEK
                     MYEKYKDKGLVILGFPCNQFAHQDPGSSSEISEFCVVNYGVTFPMFEKVDVNGKNAHP
                     IFKYLKSQLKGGLFGSKIKWNFTKFVIDKNGNPVKRFAPTVTPEKIEKYIEKIL"
BASE COUNT         4486 a         3032 c         3130 g         5150 t
ORIGIN      
        1 ttttattatg gaaaatatta atgcttttcg cgaagtaacc cctttatcga gcgaagattg
       61 ctttttcata atcgaccgaa taaagtctaa aatgacatat ccggtgcatt atcatcctga
      121 atatgagtta aattttatag aaaatggcaa agggataata cgcatagtag gcgattcgct
      181 ggaggagatt gacgatttgg aactttgcct gattggtaac gaacaactgg agcatggctg
      241 ggtggaaccg aagaatcgag aacctgagtt gatgcatgaa attacaatac agtttcatcc
      301 cgatttgttt ttagaaagtt tgcttaataa attacagttt caaagcatag ctgtgctgct
      361 tgaaaatgca aaaaaaggag tggtgttttc gagagaggcc atttacagta ttaaaaaccg
      421 acttaaactt ttatcggagc acgaatctaa ttttgaagca gttattgagc ttttttcaat
      481 acttaattta ctttctcatg acaataatta ccgtattctt tgcaattcat cttttgtcaa
      541 gaacgatgat gtttcagaga gtcgcagagt tcagaaggcc atcaattaca ttaaaatgca
      601 ttatacagaa aaattaagtt tgaacgatgt agccaatcag gtaaatatga caactacatc
      661 gtttagtagg tttatgaaat cgcgcaccgg taaaaatttt attgaatacc tgaatgattt
      721 gcgactcgga tcagcagccc gaagtttagt aaatacaaat aaaacaatat ctgagatttg
      781 ttacgagtgc ggctacaaca acctttcgaa ctttaaccga ttatttaaac aacgaaaagg
      841 gtacacccca aaagagttca gagaaaaata caagaaaatg cgaaaactga tttaaccatg
      901 ccgtgtttat taaaataaaa acacctgacg agatttttta aacctttcag gtgttttttc
      961 ttttgtatcg taatgagtct cgaaatattt aatacagtta gaaaactatc agttatgaat
     1021 aaaactctaa gaatagatta tatagtttac aaaaaataaa taataaaaca tttgagatta
     1081 tttgtataat aactaatttt gccaataaat attgatttga gataaattga gttgtgcagc
     1141 agagaaaaca aattgtggtt tacagatttt ccaaccaaaa gattaacaat cttttgtaat
     1201 caaatgcaaa catttttctt ctatacatca tttggtctgt ttctttattg ataattttag
     1261 aaaatacttt taaatactta attttttctg aaacccatat atattacata cataacacga
     1321 atgaatataa tattcagaca tgtgggttcc atcagagcat taaaaataaa ttatattctg
     1381 atgcaactat taaaccttaa caacctaaca aatttcagtt aaataaccat agtagaaatg
     1441 aaaactttta tcagggtaca agatttggga aatttacagg aagctctaca agaagctttt
     1501 gaagtgaaga agaaccgttt cgggtaccag catctgggga aaaacaaaac tttgttgatg
     1561 gtttttttta attcgagttt gagaacgcgt ctgagcaccc aaaaagcagg aatgaatctg
     1621 ggaatgaaca ccatagtgct ggatataaat cagggagctt ggaaacttga aaccgaacgt
     1681 ggcgtggtga tggatggtga taaaaccgag catattctgg aagcaattcc ggtgatgggc
     1741 aattattgcg acttaattgg cgttcgttct tttgcgcagt ttgaaaataa agcgtacgat
     1801 tatcaggaaa ccattcttca gcagtttatt caatattctg gaagacctgt gttcagcatg
     1861 gaaagtgcaa cggtacatcc attgcaggct tttgccgatc tcattaccat tgaggaatat
     1921 cgcaaaaaac cgcatcccaa agtggtcatg tcgtgggcac cccatcccaa agctctgcct
     1981 caggctgtac ccaattcttt tgccgatttt atgaacgaag ccgatgtgga atttgttatt
     2041 actcatccca aaggttatga actcgctccc gaatttgtgc gaaatgcacg tatagaatac
     2101 gatcagatga aagctttcga aggagcagat tttgtttatg ctaaaaattg ggctgcctat
     2161 caagatccga actacggaaa aattatctgc actgacagaa gctggaccgt cagcagccga
     2221 caaatggccg ttaccaacaa tgcttatttt atgcattgtc tgcctgtgcg acggaatatg
     2281 attgtaaccg atgaggtgat cgaaagtcca cagtcgttgg ttattcccga agctgccaac
     2341 cgcgaaatct cggcagaagt cgttatcaaa cgaatgcttg aaagtttata gaattttatt
     2401 gctttaatta tcaattattt aaattaattt tgttgcccgt gtgggtttaa aactatggat
     2461 gtagaacaag ttattgcaag tatcaggaat attcccgatt tccctaaaaa aggagttctt
     2521 tttaaagata ttactacggc catgaaggat gccgaagtat taagatttat ttccgatgag
     2581 ctatacgagt attacaagga caaggggata acgaaagttg ttggtttgga aagccgggga
     2641 ttcatgcttg gaagcatttt ggcgtataaa cttcatgccg gatttgtact gattcgtaaa
     2701 gcagggaagt tgccggccgc tacttataga gtggattacg aactcgaata tggagtcgat
     2761 tcgctcgaaa ttcacaaaga cgccatcgaa aaggacgatg ttgtcctcct tcacgacgat
     2821 ttgctcgcca ccggtggaag tgcgaatgct gcgctgacgt tgctcgacaa tttcaaacca
     2881 aaggctattt atgtgagctt tctcatcgaa ctgaaagatt tgaagggaag agatcgcctc
     2941 aaaggtgtgc aggatgtgca ttctctcatt cagttttgat tttttcaata aaagatatag
     3001 gttttttcga cataatatcg aagtaattgc tccataatct cgaattagaa atgaatagcg
     3061 ataaatcgcc caacatagat atgtttcaga aaaaggaaaa acttaccatc atcaaggtag
     3121 gcggaaaaat tgtggaagaa tccgatagtt taatttccct gttgaacgac ttttcaaaaa
     3181 ttgatggtta caaagtactg gtacatggtg gagggcgatc ggcaactgct ctggctactc
     3241 gtttgggact ggaaaccaaa atgattgagg gaaggcgggt tacggatgcg gaaatgttga
     3301 aggtggttac catgatatat gccggatggg tcaacaagca aattgtggca ggtctgcagg
     3361 ctttgggatt gaatgctttg ggtcttaccg gtgccgattt gaattacatg cgttccgaaa
     3421 aaaggtcgcc ctatcctata gattatggat ttgttggcga tgtgaaggaa gtaaatgtga
     3481 gccttttaac cgatttgatc aggaaagaag tggtgccggt gttggctccg ctcacacacg
     3541 ataaatgcgg taatttgttg aataccaatg ccgatacgat tgctgccgaa gctgcaaaag
     3601 ctttttcttc tttttttgaa gttacactta tttattgttt cgagaaaaaa ggcgttttaa
     3661 tgaatgaaaa agacgaagat agtgtaattt cttctttaac accggagctt tacgaaaaat
     3721 tcgtgtctca aaaagtcatt cagggaggaa tgattcccaa aatggaaaat tctttcaatg
     3781 ctttgaagtc cggagtttca aaggtgatta ttacacgagc cgacgctatc ctcacgcatt
     3841 cgggaacaac gataacttta taaaaacttt aaattttctt gttgaggaat tgattttttt
     3901 atttctcatc atattacaac gaaaatgaca gcagaacttg caaatgaagc tattcaactt
     3961 ttgcggcaaa tgatagcaat tccttctttc agcaaggaag aaaaagagat agccgatttt
     4021 ctggaacaat atctgacaga acggggatat gtcgttttcc gacatttaaa caacatttgg
     4081 ttgcaaagtc cgggttttga tgtttctcgg ccgactatcc tgctcaattc ccacttggat
     4141 accgtaaaac ccggaagcag ttggattcgc aatccctttg aagcttctct gaaaaatgga
     4201 aaattgtttg gattgggaag taatgatgcc ggtgccagtt tggtaagttt gctgcaagtt
     4261 tttttctatc tgacgcagcg acctcaaccc tataatttga tttttgctgc aacagccgaa
     4321 gaagaaattt cggggaaaaa cggcattgaa atcatgttgc aggaatttcc aaaggttgat
     4381 tttgctattg ttggcgaacc tacaggaatg aatatggctg ttgccgaaag aggattaatg
     4441 gtgatagacg caacggtaac gggaaaatcg ggtcatgcgg cgcgtgaaga aggtgaaaat
     4501 gctatttaca aggcgttgga tgcaatcaat tggtttaaaa attatcgttt tgctaaagtt
     4561 tctccgcttc ttggaccggt gaagatgacg gtaacccaga taaatggtgg cattcaacac
     4621 aatatgattc ccgataagtg tacgtttgta gtagatgtga gaagcaacga actttattcc
     4681 aatgaagaaa ttctggaaat tatccatgaa aatgtcgatt gccgtattga acctcgttct
     4741 gttcgactgt gttcaactgc cactcagccg gatcatccca ttattaagag agcgcagcaa
     4801 atgaacctta cgttatacgg ttctccaacc ctttccgatc aggcattaat gccgtttcca
     4861 agtgttaaaa ttggtcccgg cgattccgct cgttctcata cggcggatga gtatgttctt
     4921 gtagaagaaa tcgagaaagg aattgaggtt tatattcaat tgctggacgg tttgcaaatt
     4981 tgagaaattt gctgaaaagg tacttcacaa agcaacgcta aacccgcaag ttttgcaaaa
     5041 tggctcagta attttgtgtt gccgaaagcc ctgtttcatt tttgtagctc tttcgcacat
     5101 aaggatgtcg gaaagttctt ccgtaaaaat atttcccagt ttcagcgatc cgcctgcatc
     5161 aaggcaacaa ggcacaactt ctcctccggc cagaatggcg atgtgatcac gtaaagcata
     5221 acaggatttg ttttcaaaga gaggttctcc gttttctgtt ggccattgaa atttaggcga
     5281 ttgctgcaga aaaatatgat tggcgagctt tatgctgttt cctttttcag gctgtgttaa
     5341 ttcatcgggt gaaagttgaa atttttcggc gattttttcc ttacacaagc tgttgaattt
     5401 tccgcttaca agattatcaa tgttccacaa acgcaaattg acataagttt tggccgaaat
     5461 ctccattgcg atgttgaaaa tattttcgaa aaagctttcc caattagtgg atggaatgtt
     5521 ttcttcggca tcatgcagcg aaatgttcag ttgacgaatg gaatggttca aaagtaattc
     5581 cttctgatta ttgagtaaag agccgtttgt ggtgatattg acattcattc cttcctgttc
     5641 ggcaacattc agaatatctt caatatgagg gtgaagaaaa ggttctccca acacatgcag
     5701 ataaatataa ctggtaaaag gtttgatttt ccggacgatt gtgtgaaaat ctttcaccgg
     5761 cataaatctc ggcggttgac ttgtagtaaa acaaaagctg cagtgaaaat tgcagctgtt
     5821 ggtgatttca atgtaaattt ttttgaacat gggttgttca cttcatttga aatcgataat
     5881 gtcatgaatt gacaaaatgc cgactacagt tgaggaagag tcattttcca taactgccag
     5941 attggtaatg ttgttttcgt ccataatttc caatgcctca ctaagcattt cattctgagc
     6001 aattttctta aatccatgtg tcataaattc ctgagcagtc agattcttta aatcgtcgaa
     6061 tcgttgaata gcacgtcgaa tatctccatc ggtaatgatg cctacggcct tttcgttgtc
     6121 atcatatacc attgtcattc ccagtcgttt gttgctgact tcataaatta catccttaaa
     6181 aagagtatgg gtataaactt ttggaatatc gtggttcatt tggtctttaa cccgtgctag
     6241 caattgccgt cccagcgctc ctccgggatg ataaagagca taattttccg ccttgaagtt
     6301 tcttctttcc atcaagcata ttgtcaacgc atcgcccata accagcgttg cggtagtaga
     6361 agttgtgggt gccagtccca aaggacaggc ttcgcgctca ataccggtat tgattatcaa
     6421 tgaagcttct cgagccagtg gagatttcgg attcccggtc atggcgataa tggtgcagcc
     6481 gatttttttc agtggagtaa tcaggttgac aatttccgga gaacttccgc tgttggaaat
     6541 caaaagagca atatcgtttc tgtcaatcat tcccaaatcg ccatgcatgg cttcagccgc
     6601 attgatgaaa atggtttgag taccggtaga ggccagtgaa gaagcaattt tatgtccaat
     6661 gatacccgtt tttcctattc ctaaaattac cagttttccc ttgcagttgt aaatcaactc
     6721 tacagcccga ttaatttcag gtccaatttt ttcggccaat ttttgaagtt ccgatatttc
     6781 ctgttcaaaa atttctttac tcctttttac aaaatccatg attttaatat atcaacagta
     6841 attttttaga atatattcca caaattcacg cagacagcct tgtccacctt ttgctttcat
     6901 ttgaataatt cccggcacgc tttttatcag ttccattgca tcggcaggac atgcagccaa
     6961 accaaccgag catagcaatt cgtaacaatt cacatcatcg ccaatatagg ctgtttgctg
     7021 caaactaatg ccttcttttt cgcaaatctc taatgctgca gccaattttc ctccgttttt
     7081 tctgcccttg tacaggtaat cgagctgaag tttgttgtat cgcatttctg caattttgtt
     7141 gtcttcggaa gtaacaatgc caacttttat tcctttcgac tgtaaaattt ttaaacccat
     7201 gccgtcacgt acatgaaatt tttttgattg gtctccgaat tcggaataat acaagcctcc
     7261 atctgtcatt actccatcta tatcggaaag caacaacttg attttttgtt tttctttctg
     7321 aggcaactgt actttttcaa ctggttgatt caccatttct ttttcggaaa ggtttttcgt
     7381 aacattgtca attaaaatgg cttgctgtaa gatgttacga acatcggaaa gataaagttg
     7441 attcgggcca tcggaaatag cataatcggg ttgagggtga acttcggcaa aaactgcatc
     7501 cacgccgaca gccagtgctg cccgcatcaa ataaggaacc atttctctgt ttcctcctgt
     7561 tgaatttccc tgccctccgg gcttttgcac ggaatgggtg gcgtcgaaaa ctatagggaa
     7621 accatatttt cgcatttcta ccaatccggt catatccacc accaaattat tgtatccaaa
     7681 ggaagaaccg cgctcacaaa gcagaatttt gtcgtttcct gcttccaaca ttttatccac
     7741 cacatttttc atgtcccagg gggccatgaa ctgacctttt tttatgttta caatttttcc
     7801 ggttttggcg gcagctatca aaaggtcggt ttgtcgagaa agaaaagcag gaatttgcag
     7861 cacgtccaca acgtcggcaa caggttggca ttgccatgtt tcgtgaacat cagtcagtac
     7921 gggaagttga aattcattct tcactttttg taaaatttca agtccgcgct ccataccaac
     7981 gccacgagcc gatttcaatg aagtccgatt ggctttgtca aaagaagatt taaaaatcaa
     8041 atggatattc aattcccgac agatttcttt cagcgttccg gccacttcca tagtcatggc
     8101 ttccgattca atggcgcaag gcccggctat gagcacaaag cgaccgccat cgcctatatc
     8161 aaactgatga agaacattta ttttttttgt ttcaatcatg aaaccgtaag tttgattttt
     8221 ttaaaaaaag gaagattatt gtttaaattg ataaactttt atccagtcca cttccaaaac
     8281 acctttatcg cccttttcct tttcgggaat aaatgaattg agtgccagat acaactcttc
     8341 atccggtata tttcttaccg ttcggtacac ttcgtaattg ttaatcatcc aaatcagttc
     8401 cttttctgtc cacactaaac tgtaaatata aaattcggaa ggatgaattc ccttgatttt
     8461 ggttccatcc atttcatttt ttgaagcatt accgacattg atgtattttc cgtcgaaatg
     8521 aaagatattg atgtgtggca acatttcatc accacccagc cagaaagcat gatgtatctt
     8581 cccgctgcaa cgaagttttg ccctgaacat tccgtatttt tggcggaaag agtcggacgt
     8641 ctgaataaca tcggaagtgt aatcgaattc cttcattata aatcctttct gaatatccca
     8701 agcgcgagca gtaacttttt cttctttggt gaaaattttt aaacgcccat cagaaactat
     8761 tacattttta cctgaattgt tggcttgctt ttcgttggca aaagagtgat cgccgataag
     8821 ctgagggctt ttataatgaa aaccatactt ccaccgagaa ctgtcaagcg tattccaatc
     8881 gaaattttct tcaaatgtca gttccagtga acgaaatatt ccgaatcttt cgggattttc
     8941 gttttcgtaa taaataatat ccggattttt tcggagttgg tgatgacgac gctgaagggc
     9001 atattctttt gtggtgtgcc atctgtcggg gttggcccaa aaatcgttcg attttttgaa
     9061 ttcgggtgaa tttacttttt ctttcagctc ttcgtattct tttaccacga aagagtcatg
     9121 ataacgaaca aaatttttgt attccttaga atgttcaaac tgtttgaatt tttttaattc
     9181 ttccgaagct tttactttgt ttcggttgcc caaatcgaga taatcaggag tttccttgaa
     9241 tttcaaaaaa tcgtttaatt ctttcgattc cagtgttttg aagtattgct gcagtgaatg
     9301 cgaattttga agttttttgt attctgaact cagtcgatat tcttccgtat ccttgtactt
     9361 cctgtttttc agaatttttt tgttttcctg aaattttgga gaactaatga ctttggataa
     9421 ttgctggtat tctgcaagtt ctgccgatct tgctatttcg ttgtaacgtt gcaacgattg
     9481 aacacgctcg ttttcacttc tttcaaattt accggtagga gtgattttcc taaaaaactt
     9541 attcccaaat agagccatat ttttttgtat ttcagtttct tattgaaagt gattggagaa
     9601 atctcttgca ttgattgatg ttatcaacaa aaacaggtcg ataattttcc atctcaggca
     9661 aaaattgttc gattttcatt ctgtttattt gcctgataaa atcatcaaag aaatgttgtt
     9721 catttactac agctatcggt ttatgatgtt ctccaaccac accggaagca attacatcca
     9781 tcatttcgtc gaaagttcca taacttcccg gtagagcaac gaaaacatca gccaattgtt
     9841 tcattttgtt ttttctctcg cttaagtctt ctacaacgat gagttctgtg ggtaatgtac
     9901 tctggcgatg cattctgata ataggctgag cgatgacgcc aataattttt cctttttttt
     9961 cggtagctcc caaagctatg tcatccattg ttcctcctgt ggaacctcca tatacaagag
    10021 taaacccgct ttcggcaata aattggccca gcagaaaagc agcttgtttg taaacaggtt
    10081 gaacttgatt actggaagaa caaaatacgg caacgttcat ttaatttctt ttttcaagcg
    10141 ctcacagctt gtttatataa atttttcctt ccacttttaa gtgttttaaa gtggaaggaa
    10201 atttttgaaa agcatcttat gcgtcaatgt tggcgtaagt ggcattttgt tcgataaact
    10261 ggcgtcgcgg caacacgtcg tctcccataa gcatcgaaaa aacatgatcg gcggaagcgg
    10321 cattttcgat ggttacctgc cgaagtgtac gtttttcagg attcatggtt gtttcccaaa
    10381 gttgcgtttc gttcatttca cccaaacctt tgtaacgctg aacatgtatg gaaccttcct
    10441 gaccatttcc gtatttttct ataaaagcct gacgttgctg ttctgtccag caatactctt
    10501 ctatttttcc ttttttgcaa aggtagagag gaggtgtagc gatatataga taaccttgtt
    10561 cgatcagttc tttcatatgt cggaagaaaa aggtcataat caacgtggca atatggctcc
    10621 catccacatc agcatcggtc atgattatga ttttatgata tcgaagttta ctgatgttca
    10681 aagccttgct gtcttcttct gttcctatag taacgcccaa agcagtatat atattgcgta
    10741 tttcttcact ttccagcact ttgtgttgca tggctttttc cacattcaaa atttttcccc
    10801 tcagcggcaa aatagcttgg aattgacggc tacgtccctg ttttgctgtt ccgccggccg
    10861 aatctccttc gacaaggaac aattcacatt tggcaggatc tctttctaca cagtcggcaa
    10921 gttttccggg tagtcctcca ccggaaaggg gcgatttgcg ctgaaccatt tcgcgtgctt
    10981 tacgagctgc atttcgagct gtggcagcga gaatcacttt ttcaacaatc attcgggctg
    11041 cttttggatt ttcttcgaga taggcactca aagcttctcc gacagccata tccactgctc
    11101 ccattacttc gttgttgcca agtttggttt ttgtttgtcc ttcaaactga ggttctgcaa
    11161 cttttatcga aatgacagcc gtcagtcctt cacgaaagtc atcgccactg atttcaattt
    11221 taaccttatc gagcattttg ctttcttcgg catatttttt cagcgttctg gtcagtcctc
    11281 ttcggaaacc ggccaaatgc gttcctccct caatggtgtt gatgttgttc acataagagt
    11341 gaatgttttc attgtaggaa gtattgtaag tcatggcgat ttctactgga atgccatttt
    11401 tttcatttct gatgtgaata atgtcatcaa tcagtttttc acgagcttcg tctatgtacc
    11461 tgacaaactc cttgagtcct tctttggaat agaaaacttc tttcctgaaa cttccatctt
    11521 cttccactgt tctctcatct acaagtgtca atcgaatacc ggcattcaag aaagccaact
    11581 cgcgaaggcg gtcggcgatg atgtcgtatt tgtaaacgac ttccgtgaaa atcgtatcat
    11641 cagggatgaa agtagtgcgt gtgcctgttg tagaagtagt tccaatttct cttacaggga
    11701 atttgggaat accgcgttca tattcctgtt cgtaaatttt accgtttctg gcaacttcca
    11761 cgcgcataaa cgacgaaagc gcatttacac aagacactcc caccccatgc aaacctcctg
    11821 aaactttgta acttcccttg tcaaattttc ctccggcgtg aagcaccgtc attacaactt
    11881 ccaatgccga ttttccttct ttttcgtgaa aatcgaccgg aataccgcgt ccgttatcag
    11941 ttacagtaat ggaattatcc ggatggatgg taacttgaat atcatcacaa tgtcctgcca
    12001 tcgcttcgtc gatagaatta tctaccactt cataaaccaa atgatgtaat cctttgatgc
    12061 ctatatcccc aatatacatg gcaggacgtt tgcgtacagc ttcgagtcct tcgaggactt
    12121 gaatgctgct ggcatcatat tcggcaatag catttaactt ttcttctgta atcatttcta
    12181 tatagttaat tttatttttt tctctattaa taagccattt ttcaaccgaa aaaagtgttt
    12241 tgaaaaaaca tacaaattta ataaaaatat ttgagataac ctcgtttttt gagcgaaata
    12301 tacaacatct catcactatt tcagaatttt aaaatcccga tttaataaag ttttttgaac
    12361 attttaacga catgcttttt gtgctcgatt attgaataaa tttatcacga aaaatggaat
    12421 gcttgttgtc gacagcagtc aatatttttg tccatgtttc tcaaaaaaag gctaactttg
    12481 cagtcgattt ttaattaaaa aaagacatgg agatagaact caatcaactt tgcctttcgg
    12541 tatgtgaaat tgcccgatcg gcgggaaaga tgatcgccga cgaacggaaa agatttgatg
    12601 acagcaaagt ggaatacaaa ggtttacatg atttggtgag ctatgttgac aaaaattcgg
    12661 aaaagctgat tgtcagccaa cttgaaaaac ttttgcctga agccggtttt ttagcggaag
    12721 aaggtacaac atcgaaagaa ggagagcgtt acaactggat tattgatccg ttggatggta
    12781 cgaccaattt tgttcagggt gttcctgttt ttgccgtaag cattggcttg catgaagcca
    12841 atgaaatggt tcttggggtg gtttacgaaa taggaagaga tgaatgtttt tatgcttgga
    12901 aaggaggtgg agcttttctc aacggtagag ccatccatgt ttctgaacgc gataatattc
    12961 aggatgcatt gctcgctacc ggtttccctt attcggagtt cagccggcta aacgaatata
    13021 tggaatttct aaagtgggcc gtaacaaatt cacgtggggt tcgccggctc ggttcggctg
    13081 ccactgattt ggcttatgta gcttgcggcc gattcgaagc tttttgggaa tacaatctta
    13141 aggcttggga tgtagctgct ggttcaatta ttgtgaagga agcaggcgga acagttaccg
    13201 atttttcagg aaaaaacaat tttttgtttg ggaaggagat ggtagctacc aatcaaaaac
    13261 tgaaacagct ttttcttgac aagctgaaag aaatgatgtt ttaaatgcac tgctaaactt
    13321 ttagtttggt tatagcaata aaggaatctg taaaaaactc ttattatgat tatcaatgaa
    13381 cgcaatacac gtcgcgaacg cttaatgcaa gcggccaatg ccatgatgac agctgcccgt
    13441 acggctccca aaggtaaagg atacgatata atcgaaatag cgatcattac tgatgaaact
    13501 attgtggaat tgtcaaatgc catgttcgag tacagtcaac aatccggatt gaaatttatt
    13561 acgcgcgatg ccgaaaatat tctgaaatcg gaagtaattt tattaattgg cacccgacaa
    13621 caaaatcaga atttgaattg tggttactgt gggttcgata catgtgccga aaagaatagt
    13681 caacctgaca ttccctgtgc tttgaacacg gtagatgtag gtattgctat tggctcggct
    13741 tgtgctgtag ctgctgacct gaaagtggat acacgtgtta tgttttccgt tggaagagtg
    13801 gcgaaagaaa taggtttgtt gcccggatgc agttccattt tcggaattcc gctcagttgt
    13861 tcgtccaaaa atccattttt cgatcgtgtt tccaaaacac cggctcctaa ataattttct
    13921 aaaataatga ctcagtccaa agagaaactc tggacggcaa gttttatcag catttgtgtt
    13981 gccaacttta tgctgttttt tgcattttac ctcttgctgc ccatacttcc actttatttg
    14041 atagaaaatt tccaagccag caaatcgctt gttggcatta tcttgtccag ttatacgata
    14101 gcagctttgc tgattcgacc tatggccggt tttttgctgg atatgtttca acggcgcccc
    14161 atttatctgt tggcttattt tcttttttcg ctctgtttta taggttatcc gctggccaat
    14221 tttttgaatt tctttttgtc cctgcgtatt gttcacggat tgtgcttcgg tatggtatcc
    14281 acagcaagta atagctttgt tattgatatt atgccttctt cgaggagagg agaaggattg
    14341 ggttattttg gcattgccaa caatttggcc atgtctattg ggccaatgat tagcttgttt
    14401 ttacacgatt attcttctta tcaaatgatt ttctttacat ccattgccag ctcattgata
    14461 ggctttgttg ttgttttaac cgtaaaagcc aagaaaacca caattcctac tataaaaaaa
    14521 tacaaggaag cgattgtgtt ggaccgtttc ttcctcatta agggaactca tgccggcatc
    14581 tgtctgacat taatgggaat tccttatggc atgttgacca cttttatagc catttacggg
    14641 aaagaaatcg gtatgtattc gggcatgggc atctttttta ctttgatggc cattggactg
    14701 attttttcga gattttttgc cggcaggatg gtggatagag gtcatttatt gaaagttatt
    14761 accgttggaa caattgtttg tacggtttcc atttttgttt tgcctgcatt gaaagatttg
    14821 tttcttcttc agcaaaattt ttccatgatt ttgttttatg tggtggccgt ttttctcggt
    14881 ttgggttatg gaatgctatt tcccgcttac aatactttgt ttgtcaactt ggcgcctcac
    14941 aatcgccggg caacggcaag ttccacattc ctgactactt gggacgtagg aataggactg
    15001 ggtttgatta ttggaggaaa acttggcgat acgcatggcg gactttcgct ttcgttttta
    15061 actgggggat tgattgcttt ttcatcgctg gtctatttta taaaaattac agcccctcat
    15121 tataagaaaa ataccatcat tacatgatgg atttaaattc ctgttttttt attattcgca
    15181 tattcaagga atataatgaa aggcaattcc caatcaaaac ttactcctca agcggctgct
    15241 taaaataaaa aagaaagtat tttttggaat accttctttc tttttaaaat aaattttatt
    15301 taattataaa tttgtttttc aacacattat aaaatttttt ctatatattt ttctattttt
    15361 tccggagtta cggtaggtgc aaatcttttt actggattgc cgtttttgtc gatgacaaat
    15421 tttgtgaagt tccatttaat tttacttcca aacaatccac ctttcaattg cgatttgaga
    15481 tatttaaaaa ttgggtgggc gttttttcca tttacatcta ctttttcaaa catcgggaag
    15541 gttacgccat aatttacaac gcaaaattca ctgatttcgc ttgatgaacc cgggtcctga
    15601 tgcgcaaact gattgcatgg aaagcccaaa ataaccaatc ctttatcctt gtatttttca
    15661 tacatttttt caagcccctc aaattgaggt gtcaatccac atttacttgc agtgttaact
    15721 accaacacgg tttttccttt aaattgctgc atggaaatat tctcaccgtt gatttttttt
    15781 gcttcgaaat cgtaaaaa
//
LOCUS       DAOSIB010000050        15785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_3889_length_15785_cov_13.186523, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000050 DAOSIB010000000
VERSION     DAOSIB010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15785
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3889_length_15785_cov_13.186523"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="PLD44_05730"
     CDS             complement(<1..1154)
                     /locus_tag="PLD44_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOF98868.1"
                     /translation="MEPKHLYFPSADIHLDILLPASKSISNRALIINALSGSRSEIRN
                     LSDCDDTKVMLRALKLDQSIIDVGAAGTAMRFLTAYLAMTEEEWTLTGSERMKQRPVR
                     LLVEALNDLGAEIEYLEKSGFPPLSIKGKKLTGGTIHFDGSVSSQYISALMMTAPYMN
                     EGLNIILEGEIISLPYILMTKNMMKEFGVEVNFLTNRIEIAPQFYKPVDFTVESDWSA
                     ASYWFEILSIAGEGEIFLKGLHQNSYQGDSKVAELFENLGVAVQYFSDGVLLTPNRKK
                     IDFFNYDFINQPDLAQTFAVTCCLKNIPFHLKGLKSLKIKETDRISALKTELAKFGFL
                     LTEPAEGELSWSGEKKDACHFEETGEISIKTYDDHRMAMAFAPAALLFPIA"
     gene            complement(1263..2429)
                     /locus_tag="PLD44_05735"
     CDS             complement(1263..2429)
                     /locus_tag="PLD44_05735"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOF98869.1"
                     /translation="MNNFNFYSPTYFVFGKGRENEAGKYVKRFGGTKVLLHFGGNSAI
                     KSGLLDRVKKSLTQEGISFVELGGVLPNPRSGLVYKGIEICKNEGVDFILAVGGGSVI
                     DSAKAIAAGALYDGDFWDFYTGKKVEKALPVGTILTIAAAGSEGSSSSVITHENGLLK
                     RSAEGNALRPVFSILNPELTFTLPAYQTACGITDMMAHVMERYFTNTREVEITDRLCE
                     GILLTIIKEAPVAIAEPTNYGARANIMWAGMVAHNDICGVGREQDWATHQMEHELSAL
                     YDVAHGAGLAVMFPAWMKYVMNHDIMRFAQFAVRVWGCEMDFQHPEITAKEGIAKFEQ
                     FLISIGMPIRFAQLGAKAEDIPTLVKNMRLGNCTVGSFVKLTEKDVAEIYKLAV"
     gene            complement(2459..4447)
                     /locus_tag="PLD44_05740"
     CDS             complement(2459..4447)
                     /locus_tag="PLD44_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOF98870.1"
                     /translation="MKNYRLLTENEIHLLTSKGCTAENWEMVRVSEGFKPDYIAEVHF
                     SGNIFLGNFNKIFVLAGGLQRHAGIRRCCLHNCTIEDDVYIDQVANYIANYHIGEGSY
                     IENVNLLVVEKDSTFGNGVKVPVMNEGGGREIPIFDFLSAPLAYVLTLYRHRPVLIQK
                     IQKLIDDYACKLKSDTGTIGKNVRIVNAGSIKNVRIGDCANIEGASCLINGSINSNAF
                     APVTIGAGVKCDDFIISSGVSVTDSTLISRCFIGQGCILGKHYSVLDSLFFSNCQGMH
                     GEATAIFAGPYTVSHHKSTLLIAGMFSFLNAGSGSNQSNHMYKLGPIHQGLAERGAKT
                     TSDSYLLWPAKIGAFTLVMGRHTKHSDTSDLPFSYLIENATDSFLVPAVNLRSVGTIR
                     DAQKWPKRDNRKDPKKLDPINFNLLSPYTIQKMMKGVEVLKNLQKISGETTEIYTYQN
                     CSIKNSSLLRGIDLYHIGIYKFLGNSLISRLKDKHFNTIEDIREILKPDTSVGSGEWI
                     DLSGLIAPKTEIEQLMKKIEDQPITLEDIQSEFEKMHEAYYNYEWTWAKDKLEIYWRK
                     PIAEVTREDLIQMVELWKNSVVTLDHLIYDDAKKEFSLNAKTGFGVDGDEQQKSLDFE
                     SVRGSFENNPFVLEVINHIKIKSELGDELLDRLKKVIN"
     gene            complement(4477..5700)
                     /gene="hflX"
                     /locus_tag="PLD44_05745"
     CDS             complement(4477..5700)
                     /gene="hflX"
                     /locus_tag="PLD44_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOF98871.1"
                     /translation="METNNYQEKTILVGLVTPQQNREKAKEYIEELAFLAKTAGAVPE
                     KIFLQQLDHPNPETFVGPGKLEQIKNYLADHPVDMAVFDDELSARQIRNLESELKIKI
                     LDRTNLILDIFAKRAKTASAKTQVELAQYQYLLPRLTRMWTHLERQQGGIGTRGPGET
                     QIETDRRIILSKIAQLKQNLKEIDRQMSTQRKNRGKLVRVALVGYTNVGKSTLMNLLS
                     KSNIFAEDKLFATLDTTVRKVIIDNLPFLLTDTVGFIRKLPHHLIESFKSTLDEVREA
                     DLLIHVVDVSHPNFEEQIQVVNQTLKEITNEEKPMILVFNKIDAYNHKAKDPDDLTPK
                     KPENISLEEIQKTWMAKIHEDCVFISATKKQNIEELKKIMYQKIKAIHVQRYPYNDFL
                     YQDFEMEENETDVVE"
     gene            complement(5815..6498)
                     /locus_tag="PLD44_05750"
     CDS             complement(5815..6498)
                     /locus_tag="PLD44_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOF98872.1"
                     /translation="MEEKKSPKASLENKKSLFILIGLVMALSFIYIALEWTAREITIY
                     EGVQTDLKTEEELDIIQTTQDETPPPPPPPAPEVIEVLNVVENTVETQTINIETEDTK
                     NKAVEIIAAPVAAPIHEEDEQVIFQVVETMPQFPGGEQALFKFLNENIKYPVIAQENG
                     IQGRVICQFVVNTDGSIVDIQVVRGVDPSLDKEAVRVIQSMPKWIPGKQRGKPVRVRF
                     TLPINFKLQ"
     gene            6792..7811
                     /gene="porQ"
                     /locus_tag="PLD44_05755"
     CDS             6792..7811
                     /gene="porQ"
                     /locus_tag="PLD44_05755"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HOF98873.1"
                     /translation="MIKRIVLFLWFLITFVALVYPQAGKGVYQFLDLPVSSRIAAMGG
                     TNVSLKDNDINFSFCNPSLLTDKTNDFLSLSMANYLADIQYGSAVFGKNFKNKHYMAF
                     GVQYVDYGTFQETTEQNEIIGEFTAKDMALYIMYAYPITEKISVGGTFKPIYSAYERY
                     SSFGAAIDAGISYNDPTNFFSAGLVLRNIGSQIKGYYSDEEGQHYEPLPFNIQLGITK
                     KLAYAPLRFTGTLHNLQHWDLSYASSTQNETKKINFFDMALRHFIFNAEFIPSNNFYL
                     TLGYNFRRAKEMKVEDFKSQAGLSFGGGIKLYKFNVGFGMSTYQVGNSVYQFSVGTSL
                     SEFGL"
     gene            7963..9114
                     /locus_tag="PLD44_05760"
     CDS             7963..9114
                     /locus_tag="PLD44_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOF98874.1"
                     /translation="MAKILIIDDERSIRNTLKDILEFEKHQVSLAENGLKGLEAAQNE
                     MFDLIFSDIKMPEMDGIELLTQLKEKDIEAPIVMISGHGNIETAVECIKKGAFDFIEK
                     PIDLNRLLITVRNALEKKDLVVETKNLKKKTAKKYEMIGQSAALENVRQMIERVAPTD
                     ARVLITGENGTGKELVARLIHDKSNRANAPFVEVNCAAIPSELIESELFGHEKGAFTS
                     AIRQRIGKFEQADNGTIFLDEIGDMSLSAQTKVLRVLQENELSRVGSDKSIKVNVRVI
                     AATNKDLKKEIEKGNFREDLFHRLNVIPIHVPTLDERKEDIPLLVEHFTELICSEEGI
                     HPKKFEPDAIKALQQRSWTGNIRELRNVVERLIILGSPMITKDDVEKFA"
     gene            9238..9687
                     /gene="rpiB"
                     /locus_tag="PLD44_05765"
     CDS             9238..9687
                     /gene="rpiB"
                     /locus_tag="PLD44_05765"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOF98875.1"
                     /translation="MKPLNELKIAICSDHAGFELKNKLIEYLKSKNVGQIKDFGAYTA
                     ESSDYPDFAHPMASAVENGEFDFGISLCATGNGINMTVNKHKGIRAALCWMPEIASLA
                     RRHNNANVLSLPARFINENEAKQIVDVFFETDFEGGRHQRRIDKISC"
     gene            9702..10460
                     /locus_tag="PLD44_05770"
     CDS             9702..10460
                     /locus_tag="PLD44_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOF98876.1"
                     /translation="MLSKNKIKLINSLNLKKFRDETNMFIAEGTKLINDLSVTFHCEL
                     LVVTPEWLENNTPIRTKELLEVNFEEFKKISNQKNPQGILAVFGKATDIALSLEEIGS
                     ELSLALDEIQDPGNMGTIIRIADWFGIKHIFASQTSADAFAPKVVQASMGALARVQVQ
                     EVDLENFLLPLKDKLPIYGTFLKGENIYTTPLSSNGIIVLGNEGNGISPAIEKLVTER
                     LFIPNYPPGNTTSESLNVSVAAALVCAEFRRRLK"
     gene            complement(10493..12919)
                     /locus_tag="PLD44_05775"
     CDS             complement(10493..12919)
                     /locus_tag="PLD44_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOF98877.1"
                     /translation="MKKVFFISFVFLFLSFEILASTVIKGRVIDARSGTPLEFVNVAL
                     YQLGSSVPSVGLTTDAKGNFEISSISSGKYILKISMVGYQAYDKEVDIRSNILELGVI
                     RLEEESKVLNEVVVVAQGSQMKFDIDKKVFSVDQNIASSGGSATDVLQNIPSVEVDNE
                     GNVSLRNDANVEVWINGKPSGLTVENRAQVLQQMPAESIESIEIITNPSAKFNPEGTA
                     GIINLVLKKNRRGGYYGSLSAGVMYPDGAKLGSNGGINLNYSSSKIDGYVNMGYRSMN
                     MKGGGWNNRYNYLENDTTLLKQNNSSENNFDGVFMRAGLDWHLNDNNTFGISGFGMTH
                     NEDGNNNINYGLYDVDNFLLKNYSRYNLDNGNHPSFHLNVDYQHEFGRKDNNIMASIS
                     YSKHNRKSDDQYIQRDYLLDLTSSDITQTTDNRNHQWEFKIDYSNKITENSRLEAGWQ
                     SNLQERNNLTSAIDNLNNGVEIFSYSDNFDYKEQIHAAYLTYGNKFDQLSLQAGLRGE
                     YLEKKINNLETQSYFELFPSFYLSYSLPEQNELQLSYTRRINRPRGRMINPFRDYSDS
                     TSISYGNPQLLPEFASSLELNYLKTWNQHSLSASAYYRFTDNVIENVRYMNGQTMEST
                     YFNITKSKNAGLELVAKNNLFKILSLTSSLNLYYSQLDSASFTSPYNTTVTIPNQDDF
                     SWSGRLLANLMLSKNTSAQITGRYLSPRIISQGERAANYSLDLGFRQTFFDRKLNLNL
                     MVRDLLNSRKWKSTTWGTDFYQVSESYFHGRMVGMTLSYNFGNMKPKQTVKKRAESES
                     PLNMEMENIE"
     gene            complement(13264..14706)
                     /gene="pyk"
                     /locus_tag="PLD44_05780"
     CDS             complement(13264..14706)
                     /gene="pyk"
                     /locus_tag="PLD44_05780"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOF98878.1"
                     /translation="MPKFTKIIATISDKRCDPDFIRELFEAGMNVVRMNSAHLNREGF
                     LKIINSVRSVSNEIALLIDTKGPEVRTTIAENDKIILHTGDTIKIIGDPDKISTKECV
                     AVSYPFFVRDLHVGDDILFDDGEIELKVAGKDEQYLYCNVLNDGVLGSKKSVNVPGVR
                     INLPSLSARDKENILFAIENNLDFIAHSFVRNKQDILDIQQILDQHNSPIKIISKIEN
                     QEGVDNIEEILEYTYGVMIARGDLGIEVPQEKIPGIQRLLIRKCVMAKKPVIVATQML
                     HSMITNPRPTRAEVTDVANAIYYRTDALMLSGETAYGKYPVESVRTMSKIAEEAEKTK
                     LTENDIPIPTATVDITSFLANAAVEASSKFGIRAIITDSFTGSTARNLAAYRGKNPVY
                     AICYRERLTRELALSYGVFPKYQEEKRTNQEYLLLALRDLLDRKLLTEQDFFCYLSGS
                     FGEGHGTTFLEINQIEKVLKALNNYQLPSF"
     gene            complement(14756..15178)
                     /gene="aroQ"
                     /locus_tag="PLD44_05785"
     CDS             complement(14756..15178)
                     /gene="aroQ"
                     /locus_tag="PLD44_05785"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOF98879.1"
                     /translation="MKHIQIINGPNLNLLGEREPSIYGNKSFNGFFEELKKQYPDVKL
                     EYFQSNSEGEIIDKLQEIGFSADGIILNAGAYTHTSIAIADAIRSIQSPVIEVHISNV
                     FQRESFRHHSFLSEVCKGCIIGFGLNSYRLAVEALLLM"
     gene            complement(15256..15537)
                     /locus_tag="PLD44_05790"
     CDS             complement(15256..15537)
                     /locus_tag="PLD44_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HOF98880.1"
                     /translation="MNLDVSVIGYLAGFCTAVAQFPQAYKVYRTKDTRSISLGMYAIM
                     TFGVSLWLIYGILIQDWPMMLANGIGLIPCTFNLIITFQNVFRERKKMI"
BASE COUNT         4867 a         3182 c         2761 g         4975 t
ORIGIN      
        1 gcaatgggaa acagcaaggc agcgggagca aaagccattg ccatacgatg atcgtcataa
       61 gttttaatag aaatttcgcc tgtctcttca aaatgacaag cgtctttttt ttctccactc
      121 catgacaact ctccttcggc aggttcggta agcaaaaagc caaattttgc caattctgtt
      181 tttaaagctg agattcgatc ggtttctttg attttcaaac ttttaagccc ttttaaatgg
      241 aacggaatat ttttcagaca acaggtaacc gcaaaagttt gagccaaatc gggttgattg
      301 ataaaatcat aattaaagaa atctatcttt ttcctgttgg gcgttaataa aactccgtcc
      361 gaaaaatatt gaacagcaac acctaaattt tcaaataatt ccgccacttt actgtcgcct
      421 tgataactat tttgatgcaa acccttcaga aaaatttctc cttctcctgc aatgctcagt
      481 atttcaaacc agtatgaagc tgccgaccaa tcagactcaa ccgtaaaatc taccggtttg
      541 taaaactgtg gagcaatttc aatcctgttt gtcaagaaat tgacctcaac accaaattct
      601 ttcatcatat tcttggtcat cagtatataa ggaagcgaaa taatttcacc ttccaaaatt
      661 atattcaatc cttcgttcat gtaaggcgcc gtcatcatca aagccgaaat atattggctg
      721 cttacacttc catcgaaatg tatcgttcca cctgtcaatt tttttccctt aattgataaa
      781 ggtggaaaac ccgacttctc aagatattct atttctgcgc ccaaatcatt cagggcttct
      841 accaacagtc tcacaggtct ttgtttcatt cgttcacttc cggtaagcgt ccattcttct
      901 tctgtcatgg ccaaataagc agtcaaaaaa cgcatggctg ttccggcagc tccaacatct
      961 atgatagatt gatccagctt caacgcccgc agcataactt tcgtgtcatc gcagtccgaa
     1021 agattccgaa tttcagatcg tgaacccgat aaagcattta tgattagcgc acgattgcta
     1081 atgctttttg aagccgggag caaaatatcg agatgaatat cggctgaagg aaaatataaa
     1141 tgtttgggtt ccataacaag aactattcat tttactattg aataaaaaaa agcaacattc
     1201 tccttatatc gaaaaggaga atattgctac aaaattactg aattgcgccg gatataaaaa
     1261 ttttaaacgg ccagtttgta aatttccgca acatcttttt cggtgagttt tacaaaactt
     1321 ccaaccgtac agttaccaag tctcatgttt ttcaccaatg taggaatatc ctcagcttta
     1381 gctcccagct gggcaaagcg gataggcatg ccgattgaaa ttaaaaactg ttcaaatttg
     1441 gctattcctt cttttgctgt tatttcggga tgttggaaat ccatttcaca gccccaaacg
     1501 cgcacggcaa attgagcaaa gcgcataatg tcgtggttca ttacatactt catccatgcc
     1561 ggaaacataa cagccaaacc cgcaccatga gctacatcgt aaagtgccga caactcgtgt
     1621 tccatttgat gagttgccca atcctgctcc cgtcctactc cacaaatatc gttgtgagcc
     1681 accattcctg cccacataat gttggcacgg gctccataat ttgttggttc ggctattgca
     1741 acgggagctt cttttataat tgtcagcaaa attccttcac aaaggcgatc ggtaatttcc
     1801 acttcccggg tattggtaaa atatctttcc atcacgtggg ccatcatgtc ggtaatgccg
     1861 caggccgttt gataagctgg caaagtaaac gtaagttccg gattgaggat ggaaaataca
     1921 gggcgcaagg catttccttc tgccgaacgt tttaataagc cattttcgtg ggtaataact
     1981 gaactgctgg atccttcgct tccggctgct gcaatggtaa gaatcgttcc taccggcaaa
     2041 gctttttcaa cttttttccc ggtataaaaa tcccaaaaat caccatcata caacgctccg
     2101 gctgcaatag cttttgccga gtcaattaca cttccgccgc caacagccag gataaaatct
     2161 acaccttcgt ttttacaaat ttctattcct ttgtaaacca aaccactacg cggattgggc
     2221 aatacaccgc ctaattccac aaaagaaatt ccttcttgag tcagcgattt cttgactctg
     2281 tccaataatc cgcttttaat ggctgaattt cctccaaaat gaagcagtac ctttgttcct
     2341 ccaaaacgtt taacgtattt ccccgcctcg ttttctcgtc cttttccgaa aacgaaataa
     2401 gttggactat aaaaattaaa attgttcata atcaataagt tttaaaaaat ctgctttttt
     2461 aattgataac ttttttcaat ctatctaaaa gttcatcacc caattcgctt tttattttta
     2521 tatggttaat gacttccagc acaaaaggat tgttttcaaa acttccacga accgattcga
     2581 aatcaagaga tttctgctgt tcatcaccat cgacaccaaa tccggtttta gcgttcaaac
     2641 taaattcttt tttggcatca tcataaataa gatgatcgag tgtaacaaca gaatttttcc
     2701 acagttccac catctgtatc aaatcttcac gggtaacttc agcaattggt tttctccaat
     2761 aaatttccaa cttatctttt gcccaagtcc attcataatt gtaatacgct tcgtgcattt
     2821 tttcaaattc gctttgaata tcttcgagcg taatcggttg atcttcgatt ttcttcatca
     2881 actgttcaat ttccgttttg ggcgcaatca gtcccgataa atcaatccat tcgccactac
     2941 ctacagaagt atcgggcttc aaaatttccc gaatatcttc aatggtatta aaatgtttat
     3001 ctttcaatct gctgataagc gaattaccca aaaacttata tattccaatg tgatacaggt
     3061 caatacctct gagtaaagac gaatttttta tgctgcaatt ttggtaagta taaatttcag
     3121 tggtttctcc cgaaattttc tgtaagtttt tcaggacttc aactcctttc atcattttct
     3181 gaatggtgta cggacttaaa agattaaaat taatcggatc gagttttttg ggatcttttc
     3241 ggttatccct ttttggccat ttctgggcat ctcgaattgt tcccacgctt cgcaaattca
     3301 ctgccggcac caaaaaactg tcggtagcat tttctatcag ataagaaaaa ggcaaatcgg
     3361 aagtatcgga atgtttggtg tgtcgcccca taaccagcgt aaaagcacca attttagccg
     3421 gccaaagcaa ataagaatcg ctggtagttt ttgctccgcg ttcggccagt ccctgatgaa
     3481 tgggaccaag tttatacatg tgattgcttt ggttggaacc gctgcctgca ttgagaaaag
     3541 aaaacattcc tgcaatgagt aaagtagatt tgtgatgcga taccgtataa ggaccggcaa
     3601 aaatggcggt tgcttctccg tgcatgccct gacagttgga aaagaaaagt gaatcgagca
     3661 cagaataatg tttccccaaa atacaaccct gaccgataaa acaacgggaa atcaatgtgg
     3721 aatcggtaac cgaaactcca gaactgataa taaaatcgtc gcattttaca ccggctccaa
     3781 tggttacggg agcaaaagca ttactgttga tgcttccgtt gatcaggcac gaagcacctt
     3841 ctatattggc acaatcgcca atgcgaacat tttttatact gccggcattc acaatcctga
     3901 catttttacc aattgtgccg gtatcagatt ttaatttaca agcgtaatca tcaataagct
     3961 tctgaatttt ttgaataagc accggacgat ggcgatataa agtaagtaca tatgccaacg
     4021 gcgccgaaag aaaatcaaaa atcggaattt ctcttcctcc tccttcattc attacaggca
     4081 cttttactcc gttgccaaaa gttgaatcct tttctaccac cagcaaattt acattttcaa
     4141 tgtaagaacc ttcgccaata tgatagttgg ctatataatt ggctacctga tcaatataaa
     4201 catcatcttc tatcgtgcaa ttatgcagac aacaccgacg aatgccggca tgtctttgca
     4261 atcctcctgc caacacaaaa attttattga agttaccgag aaaaatattt ccggaaaaat
     4321 gaacctccgc aatataatcc ggtttgaaac cttctgaaac tctcaccatt tcccagttct
     4381 ccgccgtgca acctttagaa gtgagcaaat gaatttcatt ttcagtaaga agtcgataat
     4441 ttttcataag tgtaagaatt gaattgttaa tttattttac tcaaccacat ccgtttcatt
     4501 ttcctccatt tcaaaatcct gataaagaaa atcgttgtaa ggataacgtt gaacatgaat
     4561 agccttgatt ttctgataca taattttttt cagttcctcg atattttgtt tttttgtggc
     4621 tgaaatgaac acacaatctt catgaatttt agccatccaa gttttttgga tttcctccaa
     4681 agaaatattc tcaggttttt tcggagtcaa atcatcaggg tctttggctt tgtgattgta
     4741 agcatcaatt ttattgaaaa ccaggatcat gggcttttct tcattggtaa tttccttcag
     4801 cgtttggttg acaacctgaa tctgttcttc aaaattcggg tgagacacat ccactacatg
     4861 aatcaacaaa tcggcttcgc gaacttcatc gagtgtcgat ttgaaagatt caataagatg
     4921 atgaggcaac tttctgataa aaccgaccgt atcggtaaga agaaaaggca aattatctat
     4981 aatcaccttt ctgacagtcg tatcgagtgt agcgaaaagc ttgtcttcgg caaaaatatt
     5041 cgacttgctc agcaaattca ttaacgttga tttcccaacg ttggtgtagc caaccaacgc
     5101 cacccgcacc aattttccgc gattttttcg ctgtgtgctc atctgacggt cgatttcctt
     5161 taagttttgt ttcaattgcg caatttttga aagaataatt cttcgatcgg tttcaatttg
     5221 ggtttcgccg ggtcctctgg tgccaattcc tccctgttga cgttcaaggt gagtccacat
     5281 acgtgttagg cgaggcaaaa gatattgata ctgagccaat tcaacctgag ttttggcact
     5341 ggcagttttt gcgcgcttgg caaaaatatc aagaatcaga ttggtacgat ccaaaatttt
     5401 gatttttaat tccgattcga gatttctgat ttgtcgtgct gaaagttcgt catcaaatac
     5461 agccatatca acgggatgat ccgccaaata gttttttatt tgttccagtt ttcccggacc
     5521 gacgaaagtt tcgggattgg gatgatccaa ctgttgcaaa aaaatttttt cgggaactgc
     5581 tccggcggtt tttgccaaaa aagccaattc ttcaatatat tcttttgctt tttccctgtt
     5641 ttgttgcggt gttactaaac cgaccaaaat agttttttcc tgatagttat ttgtttccaa
     5701 ttttaatatt ctctttaaca aaaaaccgtt tggataaatt ggccaaacgg tttaatatat
     5761 ttttttcgat atgaaaggtc aaccgaaata gtataatgta aggaaaaata aaatttactg
     5821 taatttaaaa ttaatgggaa gcgtaaatct tactctaaca ggttttcctc gttgcttacc
     5881 cggaatccat ttgggcatgg attgaataac cctaaccgct tccttatcca gactaggatc
     5941 aacacctctt acaacctgaa tgtcaactat tgaaccatcg gtattcacaa caaattggca
     6001 aataacacgt ccctgaattc cgttttcctg agcaatcacc gggtatttga tattttcatt
     6061 taaaaatttg aataaagctt gttctccacc aggaaattga ggcattgttt caaccacctg
     6121 aaaaataact tgttcatctt cctcgtgaat aggtgccgcc acaggcgcag ctataatttc
     6181 aacggcttta tttttggtat cttctgtttc tatgttaatg gtttgagttt ccaccgtatt
     6241 ttccaccaca ttgagtactt caattacttc aggtgcagga ggaggtggtg gtggaggagt
     6301 ttcatcctgt gttgtttgaa tgatatccaa ctcttcttcc gtttttagat cagtttgaac
     6361 gccttcgtaa atggtaattt ctctggctgt ccactccaga gcaatataaa taaatgacaa
     6421 tgccataacc aaaccgatca gaataaataa tgatttctta ttttctaatg aggcttttgg
     6481 tgattttttt tcttccataa tgataaagtc ttttttattt ttcagatggt acaaatgtac
     6541 aatttttttt taatagttga tgttttatgg gtaaaaaaaa tttttctttt aaacaaaatc
     6601 gataacaaaa ttatattttt cataaaaagt catctccgaa cgtacctttt tcttaatttt
     6661 gccgttattt tttaattaac attggtagtt cttaaatgca ttgcaaaatt accaaaactt
     6721 tgatatattt tgcatagaac ggaaaaaatc tttattctac aatattttta aaaaattttt
     6781 tgaagaaact aatgataaaa agaattgttt tgtttttatg gttcctaata acttttgttg
     6841 ccttagttta tccacaggca ggcaaaggtg tatatcaatt tctggatttg cctgtatctt
     6901 cacgtattgc cgcaatggga ggaaccaatg tttcgctgaa agacaacgat ataaattttt
     6961 ctttttgcaa tccgtcgctg cttaccgaca aaacaaatga ttttctttca ttaagcatgg
     7021 ccaactattt ggctgacatt cagtatggaa gtgccgtgtt tggaaaaaat ttcaaaaaca
     7081 agcattacat ggcttttggc gtgcagtatg tcgactacgg cacttttcag gaaacaacgg
     7141 aacaaaacga aattattggt gaatttacag ccaaagacat ggcactttac ataatgtatg
     7201 cctacccgat taccgaaaaa atttctgttg ggggaacttt taaaccgatt tactcggctt
     7261 atgaacgtta ttcgagtttt ggggcagcca tagatgcggg catcagttat aacgacccaa
     7321 ccaatttttt ttcggcagga ttggtattgc gtaacatagg cagccaaata aaaggttatt
     7381 attccgatga agaaggacag cattacgaac cgcttccatt taacatccaa ttgggaataa
     7441 ccaaaaaatt ggcttatgcg ccgctgcgtt ttacggggac tttacacaat ttgcagcact
     7501 gggatttatc ttacgcttct tccacgcaga atgaaacgaa aaaaattaat ttttttgaca
     7561 tggctttgcg tcattttatt ttcaatgccg aatttattcc ttccaataat ttttatctga
     7621 cattgggata taattttagg cgtgcaaaag aaatgaaagt agaagatttt aaatcgcagg
     7681 caggtctttc ttttggcgga ggcattaaat tatacaaatt caatgttggt tttggcatgt
     7741 ctacctatca ggttggaaac agcgtatatc aattttctgt tggcacatcg ctctccgagt
     7801 ttggactgta gtctgtttcc tactttcgac tttttcctga aatcattcaa agcatccgga
     7861 aatttttaaa attttaaagt ccgtcagctt ctgcaattaa ataacaaaga tattttttaa
     7921 ctttgtttct gaaaaacaaa ccccgaaaga aataatttga aaatggccaa aatattaatt
     7981 attgatgacg aacgaagtat ccgtaatact ttgaaagata ttctggaatt tgaaaaacat
     8041 caggtttcac ttgccgaaaa cggtttaaaa ggccttgaag cagctcaaaa cgaaatgttt
     8101 gatttgattt tttccgatat aaaaatgccc gaaatggatg gtattgaact gcttacgcaa
     8161 ctgaaagaaa aagacattga agctcctatc gtgatgattt ccggacatgg aaatatagaa
     8221 acagccgtag aatgtataaa aaaaggagct tttgacttta tcgaaaaacc catcgatttg
     8281 aaccgtctct tgattacagt acgtaatgcg ctggaaaaga aagatttggt tgtggaaacc
     8341 aaaaatctga aaaagaagac agcaaaaaaa tatgaaatga ttggtcaatc agccgcattg
     8401 gaaaacgttc gccaaatgat tgagcgcgta gctcccactg acgcgcgcgt tttgattacc
     8461 ggtgaaaatg gaacgggaaa agaattggta gccagattaa tacatgataa aagcaaccgg
     8521 gccaatgctc catttgttga agtaaactgt gcggccattc cttcggaact gattgaaagt
     8581 gaacttttcg gacacgaaaa aggagctttc acttctgcca tccgacaaag gatcggtaaa
     8641 tttgaacaag ccgacaacgg aaccatcttt ctcgacgaaa ttggagatat gagtctttcg
     8701 gctcaaacaa aagttttgcg ggttttgcag gaaaatgaac tttcgcgtgt ggggagcgac
     8761 aaaagtatca aagtcaatgt cagagtaatt gccgcaacca acaaggattt gaaaaaagaa
     8821 atcgaaaaag ggaatttccg cgaagatttg tttcatcggc ttaacgttat cccaattcat
     8881 gttccaactt tggacgaacg aaaagaagat attcccttgc tggtagaaca tttcacggaa
     8941 ttaatctgtt ctgaggaagg tattcatccc aaaaaatttg aaccggacgc cataaaggct
     9001 ctacaacaac gctcgtggac gggaaatata cgcgaactgc gcaatgtggt ggaaagattg
     9061 attattctcg gttctccgat gattaccaaa gatgatgtgg aaaaatttgc atgaatcgta
     9121 cacaatccac tgccggtgca gcaaccaaat tttttcaaac agtcagaaaa aattttttcc
     9181 ggacaaataa atcaatgcaa ggaatttcat caatttcttt aaaattaatt caatcacatg
     9241 aaaccactca acgaacttaa aattgccatt tgtagcgatc atgcaggttt tgaacttaaa
     9301 aacaaactga ttgagtatct gaaatcgaaa aatgttgggc aaataaaaga ttttggcgcc
     9361 tatacggcag aaagttccga ttatcccgat ttcgcccatc ctatggcttc ggcagtagaa
     9421 aacggagaat ttgattttgg catttccctg tgtgctacgg gaaacggcat caacatgacg
     9481 gtgaacaaac acaaaggaat tcgcgctgcg ctttgctgga tgcccgaaat cgcatcgctg
     9541 gcacgcagac acaacaacgc caatgtgctt tcgctgccgg caaggtttat caacgaaaat
     9601 gaagccaaac aaattgtgga tgtgttcttt gaaaccgatt ttgaaggagg aagacatcag
     9661 cgacgaatag acaaaatatc ctgctgaaaa acgaaaaact catgttatcg aaaaataaaa
     9721 tcaagcttat caattctttg aacctaaaaa aattcagaga tgaaaccaat atgtttattg
     9781 ctgaaggaac aaaattgatt aacgatttat cggtaacatt tcattgcgaa cttttagtgg
     9841 ttacacctga atggctcgaa aacaatacgc ccattcggac aaaagaacta ttggaagtaa
     9901 attttgaaga gttcaagaaa atttccaatc aaaaaaatcc tcaaggtata ttggccgttt
     9961 tcggcaaagc gaccgacatc gctttatcgt tggaagaaat cggctcagaa ttatcgttgg
    10021 cgctggatga aattcaggat cccggcaata tgggtaccat tattcgaatt gccgactggt
    10081 tcggcattaa acatattttt gcttctcaaa cttcggcaga tgcttttgct cctaaagttg
    10141 tacaagccag tatgggagcg ctggcacgcg tacaagttca ggaagtcgat ctggaaaatt
    10201 ttctgttgcc attgaaagat aaactgccta tttacggaac ttttctgaaa ggagaaaata
    10261 tttatactac tcccctatct tcaaatggca ttattgttct cggaaacgaa gggaacggca
    10321 tctctccggc aattgaaaaa ttggttaccg aacgtctttt tattccaaac tatccacccg
    10381 gaaataccac ttccgaatcg ctcaacgtaa gcgtagcggc agctttggtt tgtgcggaat
    10441 ttcggcgcag actcaaataa aaagcttttg gcaactacaa tgcctttcct tcttactcta
    10501 tattttccat ttccatattc aaaggacttt ccgattccgc tcttttttta actgtttgtt
    10561 tgggtttcat gttgccgaaa ttgtaagata gagtcatgcc caccattctt ccatgaaaat
    10621 aagattccga tacctgataa aaatcggttc cccatgttgt tgatttccat tttctcgaat
    10681 ttaacaaatc acgaaccatt aggttgagat ttaactttct atcaaaaaat gtttgtctaa
    10741 aacccaggtc aagagaataa ttggcagctc tttccccctg tgagatgatg cggggagata
    10801 aataacggcc ggtgatttgt gcagaagtat ttttactgag cattaaattt gccagcaatc
    10861 ttccactcca tgaaaaatca tcctgatttg gaatggtaac agtcgtattg tacggactgg
    10921 taaatgaagc cgaatccaac tgactgtaat ataaatttaa agaagaagtc agactcaata
    10981 tcttaaacaa attgttcttt gcaaccaact caagaccggc attttttgat tttgtgatgt
    11041 taaagtacgt actttccatc gtttgtccgt tcatataacg cacattttca ataacattat
    11101 ctgtaaagcg ataataggcc gacgccgaaa gagaatgttg attccatgtt ttcaaataat
    11161 tgagttccaa cgaactggca aattccggca aaagttgtgg atttccatac gatatgctgg
    11221 tcgaatccga ataatctctg aaaggattga tcatgcgtcc tctgggacgg tttattcgac
    11281 gggtatagct caattgcaac tcgttttgtt caggtaatga ataggaaaga taaaaactgg
    11341 gaaataattc aaaataagat tgagtctcca aattgttgat ttttttttcc aaatattcac
    11401 cccgaagtcc ggcttgcaaa ctcaactggt caaatttgtt accataggta agataggcag
    11461 catgtatctg ttctttataa tcgaaattgt ctgaataact aaaaatttcc actccattgt
    11521 tcaaattgtc tatggcactg gttaaattgt ttcgctcctg aagattactt tgccaaccgg
    11581 cctccaatct gctgttttcc gtaattttat tcgagtagtc gattttaaat tcccactgat
    11641 gattccgatt gtcggtagtt tgtgttatgt cgcttgaggt aagatctaat agataatccc
    11701 tttgaatata ctggtcatca gattttctat tatgttttga ataagaaata ctggccataa
    11761 tattattatc ttttcttcca aattcgtgtt gataatcaac attcagatga aagcttggat
    11821 gatttccatt atccaaatta taacggctat agtttttcag aagaaaatta tcaacatcat
    11881 ataaaccata attaatgttg ttgtttccat cttcattgtg tgtcattcca aagcccgaga
    11941 taccaaaagt gttattatcg ttcaaatgcc agtcgagacc tgcacgcata aagacaccat
    12001 caaaattatt ttctgatgaa ttattttgtt tcagcaaagt agtatcattt tcgagataat
    12061 tgtatcgatt gttccaaccc ccgcctttca tgttcatcga tcgataaccc atgttgacat
    12121 aaccatctat tttgctgcta ctataattta aatttattcc gccattacta cctaattttg
    12181 ctccatccgg atacattact ccggccgaaa ggctaccata ataaccacca cgtctgtttt
    12241 ttttcaaaac aaggttgata atgccggctg ttccttcagg attgaatttt gcagaaggat
    12301 tagtaataat ttcaatagat tcaatgcttt cagcaggcat ttgttgtaaa acttgagcac
    12361 gattctcaac tgtaagtcca gaaggtttac cgttgatcca aacttcaaca tttgcatcgt
    12421 ttcttaaaga cacatttcct tcattatcaa cttctaccga agggatattc tgaagaacat
    12481 ccgttgctga gcctcctgaa gaagcgatgt tctgatcaac actaaaaact tttttatcga
    12541 tgtcgaattt catttgagaa ccttgagcta caacaaccac ttcgttcaat actttcgatt
    12601 cttcttccaa acgaatgact ccaagttcta aaatattgct tcttatatct acttctttgt
    12661 cataagcctg atagcctacc atactgattt tgagaatata tttaccactg ctaatagagg
    12721 aaatttcgaa attccctttt gcatcggtgg ttaatccgac agagggaaca gaagaaccta
    12781 attgatataa agcaacattt acaaactcta atggagtgcc agacctggca tcaattactc
    12841 ttccctttat aaccgttgaa gctaagattt caaaacttaa gaataaaaaa acaaaggaaa
    12901 taaaaaatac ttttttcatc agaatataat ttatttttta aaggtctgat ggaacccaca
    12961 tgtctgacat attatattca ttcgtgtttg tgtatgcaat acatgtgggt ttcatacaaa
    13021 tatcttttcg ttttataagt gtttatgaaa acttacatat ttaatagact tcaaaacacc
    13081 tacaaagttt aatcttcgat aggtttttaa atgttatgta attgcataaa cttctataaa
    13141 aaatactatc caatcattta ttaacgaaat gataatcaca tttacttatt cagttaaacc
    13201 attttagcgt tttaactgtt tgatgttttt cggatattag aaaaaaggaa tttttaaata
    13261 agtctaaaaa ctgggtaact ggtaattatt caatgcttta agaacttttt caatttgatt
    13321 gatttccaga aaagtggttc cgtgaccttc tccaaaactt ccgctcaaat aacagaagaa
    13381 atcctgttcg gtcaaaagtt ttctatccag taaatccctt aaagccaaca ataaatattc
    13441 ctgattggtt cgtttttctt cctgatattt gggaaaaaca ccataagaca aagccaattc
    13501 gcgcgtcaat ctttcacgat aacaaatggc ataaacagga tttttccctc gataggctgc
    13561 caaatttctg gctgtcgatc cggtgaaaga atcggtaata atagcccgaa tgccaaattt
    13621 actgctggct tccacagcag catttgccag aaaagaagta atatcgactg ttgccgtagg
    13681 aatcggaata tcgttttccg tcagtttcgt tttttctgct tcttcggcaa ttttcgacat
    13741 ggttcgtacc gattcaacag gatacttgcc ataagcagtt tctccgctca acatcaaggc
    13801 atcggtgcga taataaatgg cgttggccac atccgtaact tctgcccgcg tgggacgtgg
    13861 attggtaatc atggaatgaa gcatctgagt agccacaata accggttttt ttgccataac
    13921 acatttcctg atcagaaggc gctgaatacc cggaattttt tcctgaggca cttctattcc
    13981 caaatcaccg cgagctatca ttaccccata ggtgtattca agaatttctt ctatattgtc
    14041 cacgccttcc tgattctcga ttttcgaaat aattttaatc ggactgttat gctgatcgag
    14101 aatttgctga atatcgagaa tatcctgctt gtttcgaaca aatgaatgag ctataaaatc
    14161 aagattgttt tcaatggcaa ataaaatatt ttccttgtcg cgtgctgaca aggatggcag
    14221 attgatgcgt acaccgggaa cattcacact ttttttactt cccaatacac catcattcaa
    14281 cacattacaa taaagatact gctcatcttt ccctgccact ttcaactcta tttctccatc
    14341 gtcaaaaaga atatcatcgc ctacatgtaa atcgcgcaca aaaaacggat aactgaccgc
    14401 aacacattct ttggtcgaga ttttgtcggg atcgccaata attttaatgg tgtcgccggt
    14461 atgtaaaatt attttatcgt tttcggcaat ggtcgttcgc acttcaggac ctttggtatc
    14521 gatcaacagg gcaatttcgt tgctcaccga acgaacgcta ttaataattt tcagaaatcc
    14581 ttccctgttg agatgtgccg aattcatccg aaccacattc atccctgctt caaacaactc
    14641 gcgaataaaa tcgggatcgc atcttttatc gctaatggta gctattattt ttgtaaattt
    14701 tggcataaaa aattaatgaa taaatttgaa attcaaccag cttcctaatc catttttaca
    14761 taagcagcaa agcttctaca gccagtcgat aagagttcag tccgaatccg ataatgcaac
    14821 ctttacatac ttcggacaaa aaagaatgat gacgaaaact ttcgcgctga aaaacattgg
    14881 aaatgtgcac ttcgatcaca ggcgattgaa tggagcgaat ggcatcggca attgcaatag
    14941 aagtatgcgt ataagctccc gcatttaaaa ttattccgtc agcggaaaat ccaatttctt
    15001 gcaacttatc gataatttca ccttcactgt tggattggaa atattccaat ttcacatcgg
    15061 gatattgctt tttcaactct tcaaaaaaac cattaaaact tttgtttcca taaatggaag
    15121 gctctctttc tcccaaaaga ttgagattag gcccattgat aatttgaata tgtttcatag
    15181 cgtattgata atgaaacgtt aaattttttc tcagtttcga aaacctaaac tgataatttt
    15241 tttaattact ccaatttata tcattttctt tcgttcacga aaaacgttct gaaaggttat
    15301 gattaaattg aatgtacagg gaattaaccc aattccgttt gccaacatca ttggccagtc
    15361 ctgaatcaaa attccgtaaa ttagccacag ggaaacgcca aaagtcataa ttgcgtacat
    15421 acccagcgaa atacttcgtg tatctttggt tctgtaaact ttgtatgctt gtggaaactg
    15481 tgccacagca gtacaaaacc ccgccaaata gccaatcacc gaaacatcta aattcatgat
    15541 aagattttta tgcaaaaata agaaaaaatg caataatgaa aaaccgacaa acacgagaag
    15601 ttaacgtttc atttcaatat ttaatgtctt cactattttc aaataaagat aacagagaaa
    15661 aagacggatt tctctgcaaa acctgttact agtttaacaa ttttttttgt tcgtcttctc
    15721 tttacaatca aaattgaaaa agctgcatca gaattcaaaa ttatcgtata tctttgttat
    15781 tgaac
//
LOCUS       DAOSIB010000051        15546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_3950_length_15546_cov_11.207475, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000051 DAOSIB010000000
VERSION     DAOSIB010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15546
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3950_length_15546_cov_11.207475"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLD44_05795"
     CDS             <1..219
                     /locus_tag="PLD44_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOF98881.1"
                     /translation="TTTIGFYLPNTVNAATIYVYDMNGVQLKSYPITERGKGNVIIQG
                     SELIAGMYLYALIADGEVIDTKRMILTK"
     gene            230..859
                     /locus_tag="PLD44_05800"
     CDS             230..859
                     /locus_tag="PLD44_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98882.1"
                     /translation="MKTLTMSFLLFIIVSTSNTFAQYHVQNNDSSKWGYYLQIIDTNY
                     IPVIVSVNELGYITLHTGITDFDAIFAKYKITDFFQYAPTAATDWLRQVYVVVCDSGQ
                     TQLGIDLQEKYSSVIVRIEYYYHNPILTGGLINNIEKFGQRGNILWLGENNGLSKTVY
                     FFDINGKIIFSTRTFNESIDPSCILTNGFFIYKIKVGESIKKGLYYNLK"
     gene            868..2322
                     /locus_tag="PLD44_05805"
     CDS             868..2322
                     /locus_tag="PLD44_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOF98883.1"
                     /translation="MKKLILLCAILIISIVPLYVFSQNAHFYIHIKDSKYIPTVTDLK
                     DSSGFLKVSSKIDKLNMVVRKYKFKEFKQAFPTAKTDWLKEIYYVDCDNEDFENDIKK
                     QFVSQMPLIERLCSPQTTGSFTPNDPIFREGKQTNLDLIHAEEAWDIAKDYPKIKVAI
                     NDNGFQLDHEDLQYDSIDGQNTTNLHGTFTAGCLGAKIDNSIGLASIGSLSSQITYST
                     GSKWGDNNAILLLAQAGYRVINCSWFHSCTYSSIEAALYDEIRNTWNCVVVFGAGNGA
                     GHCNYGKTYPPAYESCLSVTSVGHIYDRGVLENGIAKNWKDVHERYIGDPSSTHNHNE
                     AVDICAPGYDIYSTATSNTYFTSNGTSFAAPQVAGVAAMIIAVNPSLSSNQVVDILKS
                     TADTTIYDIPENTNYIGQLGSGRLNAYKAVKKACSLDFNNTAFVGNSNETGCYITATN
                     CIIESTANVVFDATKEVNLQSGFEVQLGSTFEAK"
     gene            2674..3102
                     /locus_tag="PLD44_05810"
     CDS             2674..3102
                     /locus_tag="PLD44_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98884.1"
                     /translation="MASTSETGHAKNVANFNELISFVSGYGETYNPSKASIKLTALQT
                     LLADAKSAMDAVNSAMPAYSNAVSAREAAFEPLNKLITRVMNAVKATDISSQVEESVK
                     TLVRKIQGTRSTAKKTETQKADMTAEGKEVKEISTLQDVL"
     gene            3626..3799
                     /locus_tag="PLD44_05815"
     CDS             3626..3799
                     /locus_tag="PLD44_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98885.1"
                     /translation="MLPEVSNAVNTLNTSGSVIVKSTDRITFEAGQEIILNTGFEVQQ
                     GGQLDVNIVTCGQ"
     gene            3817..4527
                     /locus_tag="PLD44_05820"
     CDS             3817..4527
                     /locus_tag="PLD44_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOF98886.1"
                     /translation="MKRILIVLVFNLLFSHFLFAQENSRINSFFNDTLKGKWILESNC
                     LLEYQRCDSIGKGIEYIIFNSINSQGFVKVVTNGSWSFENGDSTKLYLPEKTIFGETW
                     VMDDYIFYSYNKICVINENIDKLFLSTNSFIGFAKSYIRDKSYLGLQSKINIPITIYP
                     NPFIDKINVSYDIPNSTNRVLINIYSSTGLLLKKIEIAENGQFKKTISLEDLNKGIYF
                     GVVSLNDKWINNIKLIKK"
     gene            complement(4812..5930)
                     /gene="buk"
                     /locus_tag="PLD44_05825"
     CDS             complement(4812..5930)
                     /gene="buk"
                     /locus_tag="PLD44_05825"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOF98887.1"
                     /translation="MSENIINLHKNVDTNPTPMFKILSINPGSTSTKFAVYEDEKILF
                     LHTILHSTEELKSFEKIIDQLDFRKNLILNSLKHDGVDLQTLSAIVGRGGLIRPVASG
                     VYLVNEKMIADLREAKKGEHASNLGGIIAYEISRDIPECKPFIVDPVVVDEMQDVARI
                     SGLPQFPRRSMFHALNQKAVARKYAASVSAGYENLNLVIAHLGGGISVSAHAKGKVID
                     TNQGLGGYGPFSPERSGTIDAGLLVDFCFQGQASKEEIKKMLVGRGGLIAHLGTNDAY
                     LAEQKAVNGDTHAKLIMEAMAYNVAKEIGAMCTVLKGKVDAIILTGGISRSEWFVNYV
                     KEMVSSFGKLVVYPGENEVEALAMGALRVLRSESYKEY"
     gene            6174..6641
                     /gene="rimP"
                     /locus_tag="PLD44_05830"
     CDS             6174..6641
                     /gene="rimP"
                     /locus_tag="PLD44_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HOF98888.1"
                     /translation="MIEQTTILQIVENFVSGTDFYIVEVKVSPENQILVVFDSFEGIS
                     IDDCEALNNFIESKLDREQEDYELEVSSPGLTEPFKVLKQYEKNKGNEVEIVTKKGEK
                     FSGILTEVNFDNCTLQIEKLIKPEGAKRKIKVTEKVPFRYEDIKTTKYILRFK"
     gene            6645..7901
                     /gene="nusA"
                     /locus_tag="PLD44_05835"
     CDS             6645..7901
                     /gene="nusA"
                     /locus_tag="PLD44_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOF98889.1"
                     /translation="MSKKETVNLIDTFSEFKELKNIDRSTLISVMEESFRNIIAKNYG
                     TDKNFDVIINPDKGDFEIYRNRTVVADDEVSDPNLEISLSEAQKIDEDYQIGEEVTDT
                     VDISDFGRRAILTLRQTLQSKILELQKDNVYTKYSKMVGQIVTAELYQIWKKEMLLMD
                     EEGNELYLPKSEQIPTDFYRKGDMVKAVVAMVENKNNNPRVILSRISPVFLERLFENE
                     IPEIHDGLITIKKIARIPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNEN
                     IDVINYTNNISLFISRALSPAKISSIRINEAEKKAEIYLKPEEVSQAIGKGGLNIKLA
                     SMLTGYTLDVFRELDETETEDIYLDEFNDEIDQWVIDTLKSIGCDTAKNVLAIPREDL
                     IRRTDLEEETIDEVLDILRSEFEDEN"
     gene            7966..11019
                     /gene="infB"
                     /locus_tag="PLD44_05840"
     CDS             7966..11019
                     /gene="infB"
                     /locus_tag="PLD44_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOF98890.1"
                     /translation="MPIRLSKACKDLNVGMSTAVEFLAKKGHKLSADPNLKLEDELYL
                     LLAKEFNKDMALKIESERISKERQEKEKAETVGLEEYVHPKTAPKPAEIIPTVIPEEQ
                     KPHIKSVGHIDLEETGKKVKTKEKEKTEIVDSIPVEPIKEPVVAEKEKIEPDATFTHE
                     VKAVVTEEPVKPEKPEEIIQEQKSQPEEFEVTEKILPDSTEIKAEKIEEKIETQVITS
                     PEISKHRTTQESESSNLEEVNVNEAEIEISELTEEVFYTEIPVLDTNITVVGSIDLDA
                     LNQRVRPKPKSKEQRKREKEEKIQLEAEAKKIAQKGKKVIKLPGEAEDAEDTKDHHKK
                     KRERFTKQKVDISKVSGNANPEELERPKKLNLSPKGTKDKFKKSIKPAISEEDIQKQI
                     KETLARLATSNKKTKGSKYKKDKREIINARQQQLAEMEQSNEKVLKLTEFVTVNELAN
                     MMDVPVNDVISTCMNLGLIVSINQRLDAETINILADEFGFTTEYVSHDVIEALAENEP
                     DDEENLQPRPPIVTVMGHVDHGKTSLLDYIRKTNVIAGEAGGITQHIGAYNVVLDNGQ
                     RITFLDTPGHEAFTAMRARGAKVTDLAIIIIAADDNVMPQTVEAINHASAANVPIVFA
                     INKVDKPDANPEKIKEQLANMNYLVEDWGGKYQSQEISAKKGIGVPELLEKVLLEAEL
                     LDLKANPNRRAIGSIIESSLDKGRGYIATVLVQNGTLHQGDVVLAGTHFGKVKAMFNE
                     RNQRISEARPSEPVLILGLNGAPQAGDNFVVMESEHEARELALKREQLQREQTIRTKK
                     HFTLDELGRRIALGDFKELNVIVKGDVDGSVEAISDSLLKLSTEKIQVNIIHKAVGAI
                     SDSDVLLAAASNAIIVGFQVRPTPSARKMAEKEEIDIRLYSIIYDAIEEIKSAMEGML
                     SPEIKEEITATLEVRDVFKITKVGTVAGCIVKEGKIKRTNKVRLIRDGIVIFTGEIES
                     LKRFKEDVKEVAVNYECGVSIHNFNDIHVGDLIESFEEMEVKSEL"
     gene            complement(11288..11926)
                     /locus_tag="PLD44_05845"
     CDS             complement(11288..11926)
                     /locus_tag="PLD44_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF98891.1"
                     /translation="MNIKTFIFNPFRENTYIVSDVTGEAIIIDAGCLTNREKQAVKNY
                     IENNKLVLKHLLNTHLHIDHQFGNRYIYDNYHISPEACKEDEFLIETMHEAAKFFGID
                     LHEEPPAIGSYIIENQEIKFGNSILKALHVPGHSPGSMAFYSEQNGLVFTGDVLFYGT
                     IGATHFEKGDHDLLIRSIWEKLMVLPDETLVYSGHGQPTTIGNEKNFNPYLV"
     gene            complement(11965..12588)
                     /gene="rsmG"
                     /locus_tag="PLD44_05850"
     CDS             complement(11965..12588)
                     /gene="rsmG"
                     /locus_tag="PLD44_05850"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOF98892.1"
                     /translation="MELINKYFPDLSSHQKAQFEALYPLYKDWNEKINVISRKDIQNL
                     YEHHVLHSLAIAQYIRFKPNSSILDVGTGGGFPGIPLAILFPEVKFVMIDSIGKKIKV
                     AMSVAEAIELKNSEFKYLRAEEEKGKYDFIVSRAVMPLEDLLKMTKKNIRKTSINALP
                     NGIISLKGGSLEQEIKPFKKIIEVASISDFFKEEYFKDKKIVYLPVV"
     gene            12945..13727
                     /locus_tag="PLD44_05855"
     CDS             12945..13727
                     /locus_tag="PLD44_05855"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOF98893.1"
                     /translation="MNHIPKLSHILVLILVVQLSSCITARKINYLQQPNASIPAYKDT
                     FSFNDYRLKKGDRVFIKIYSWDDKTNALLNGSSNIEQLVMSGSSSGNELYTYLIQPSG
                     KVNLPMVGEIYLEGQTVREAKETLEKFIQPVFDISSVDVRIAGRFFSVIGGGATGRYP
                     LNREKINIFEALAMAGDIDTYGDRSKIRIIRETNSGTQIKVFDVRSVDIINSEFYYVE
                     PNDVIYIQDMNEQFFSVTSFASLLSTVFATFSFGIFIYGLTR"
     gene            13754..14500
                     /locus_tag="PLD44_05860"
     CDS             13754..14500
                     /locus_tag="PLD44_05860"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOF98894.1"
                     /translation="MKHFLHIIFTILTVLSFSSCYNYRSIGLLQENKSLPVYSKADYS
                     DYEICVNDEIIFRLITTDETISKLISSNQSGNSNQMISYRVYPDGTVDIPFLNRVQVK
                     GLTINEAENLLKEEFREIIPDAEVKLSIANKSFTVIGEAGTGVFPVYKDKLTIYQALS
                     LSGDLKLSGDFRHVKILRPVEGKTEILEFDIRPASVIESKYYYVYPNDIIYVQRASSS
                     FFKMNNYSSFLGLISSSLTLLFSVLYYIKL"
     gene            14535..>15546
                     /locus_tag="PLD44_05865"
     CDS             14535..>15546
                     /locus_tag="PLD44_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98895.1"
                     /translation="MNPDTGSINQNFTEEESSFDFQKWLHLFIKNWYLFLIFIPFFLI
                     GGYLKNRSWMPLYQSVGTIIINENKYRSSSQGFMQGFGLEEAYQNVNNQVIILNSYDL
                     IGKVVDSLPFMKVDYLTIGRFKTRNLYTTTPIIIEADYVAPEAYELLFKISLHPNGTF
                     TISPHNNNSMPSSPINGHIGVPLQHNLFFMTVNSVSDNVKEFEMYFRFRTRETLIEDF
                     KSRLSSRYVIEGSSILEISLISETPDRDVDFINKLSEIYLEENLKKKNDVASKTIQFI
                     NEQLSEVSKSLSVSEDEMTRFRRDNQIIDISSHGSELMSKANSLDEKKAELKLKETYL
                     NYL"
BASE COUNT         5349 a         2566 c         2896 g         4735 t
ORIGIN      
        1 acaactacta ttggttttta tttaccaaac actgtcaatg cagctaccat ttatgtctat
       61 gacatgaatg gagttcaatt aaaaagttat ccaatcaccg aaagaggaaa aggaaatgta
      121 attattcaag gctcagaact tatcgctggc atgtatttat atgctttgat tgccgatggc
      181 gaagtgattg ataccaaacg aatgatatta acgaaataaa ttcaaagtta tgaaaacact
      241 cacaatgtca ttcttactat ttattattgt ttctacatca aatacatttg cacaatatca
      301 tgtgcaaaac aatgacagta gtaaatgggg atattatttg caaattattg atactaatta
      361 tataccggta attgtaagtg taaatgaact gggctatatt acccttcata ccggaataac
      421 ggatttcgat gctatatttg ccaagtacaa aattacagat ttctttcaat acgctccaac
      481 agctgctacc gattggctta gacaagtcta tgtagttgtt tgtgattcag gtcagacgca
      541 attgggaata gatttacaag aaaaatatag cagtgtcatt gttagaatag aatattacta
      601 tcataatcca atcttaacag gtggattaat taataatatt gaaaaattcg gacaaagagg
      661 aaatatatta tggttgggag aaaataatgg attaagtaaa actgtctatt tctttgacat
      721 aaacggcaaa ataatatttt ctactcgtac atttaatgaa tcaatagatc cttcttgtat
      781 tttgacgaat ggtttcttta tctacaaaat taaagttgga gaaagtatca aaaaaggctt
      841 gtattataac cttaaataaa ctaaaaaatg aaaaaactaa tattattatg tgcaattctg
      901 ataatttcaa ttgtaccgtt gtatgtattt tcgcaaaatg cacactttta tattcacatt
      961 aaggatagta aatacattcc aactgtcaca gatttaaagg atagtagtgg atttttaaaa
     1021 gtttcatcga aaatagataa attaaatatg gttgttcgaa aatataagtt taaggagttt
     1081 aaacaagctt ttccaacagc aaaaacagat tggctaaaag aaatatatta cgtagattgt
     1141 gataatgaag actttgaaaa tgacatcaaa aagcaatttg tttcacaaat gccattaata
     1201 gaaagacttt gctcaccaca aaccactgga agttttacac caaatgaccc catctttaga
     1261 gaaggtaaac agacaaatct cgatttaata catgctgaag aagcatggga tatagcaaaa
     1321 gattatccta aaatcaaagt tgcaattaat gataatggat ttcaattaga ccacgaagac
     1381 cttcaatatg atagtataga tggtcaaaat acaactaatt tacatggcac ctttaccgcg
     1441 ggttgtttag gagctaaaat tgacaattct attggattag cctctattgg aagtctaagt
     1501 tctcaaataa cttattctac tggctcaaaa tggggtgata ataatgccat tttgctatta
     1561 gctcaagcag gctatcgggt aataaattgt agttggtttc atagttgtac atattcttct
     1621 attgaagcag ctttgtatga tgaaattaga aatacatgga attgtgtagt tgttttcgga
     1681 gcaggtaatg gtgctggaca ttgtaattat ggtaagactt atccacccgc atatgaatct
     1741 tgtctatctg ttactagtgt tggacatatt tatgatagag gggttttaga aaatggaatt
     1801 gcaaaaaatt ggaaagatgt acatgaaaga tacattgggg atccaagtag tacacataat
     1861 cataatgaag ctgttgatat atgtgcacca ggatatgata tatactcgac tgcgacatcc
     1921 aatacatatt ttacttcaaa tgggacatct tttgctgctc cacaagtagc tggtgttgca
     1981 gctatgataa ttgctgtaaa tcctagtctt tcatcaaatc aggtagtaga catattaaaa
     2041 tctacagcag atacaacaat ctatgatatt cctgaaaaca caaattatat tggtcagcta
     2101 ggatcaggta gattaaatgc ttataaagct gtaaagaaag catgttcttt agattttaat
     2161 aatactgctt ttgtaggtaa tagtaatgaa acaggttgtt acattacagc aactaattgc
     2221 ataattgaat ctacagcaaa tgtagttttt gatgcaacaa aggaggttaa tttacaaagc
     2281 ggttttgaag ttcaattagg tagtactttc gaagctaaat aattattctt tatgtaggag
     2341 tttaatcgcc ccaaaagcaa agaaaaaaaa ccacagcaag cgagaatata tattcaaaag
     2401 tcttgctgtg gcttttatag ctttttgata ataggcagat cggtcacagt acattgttta
     2461 ttgctcgttt agacaattga actgcatttt gaaagaaaaa acaatgaact gtgaagtatt
     2521 tattgcttag caatacttaa taggttaatt ctcatagata aaaaatgtga aaaatgtttg
     2581 taaattacct acaaaataaa tcttaaatac aatctattta actatatttg cagcataatt
     2641 atttcctttt ataaatctaa aatctaactt attatggcaa gtacttcaga aacaggacac
     2701 gcaaaaaacg tggcaaactt caacgaactg atatcgtttg tttcgggtta cggagaaacc
     2761 tacaatccct caaaagcctc cataaaatta accgccttgc aaaccctcct ggcagatgca
     2821 aaatccgcta tggatgcggt gaactcagca atgcccgctt acagcaacgc cgtttcggca
     2881 cgtgaagccg cctttgaacc attaaacaaa ctcatcaccc gagtaatgaa cgccgttaaa
     2941 gccacagata tctcttccca agtagaagaa agcgtaaaaa cgttggttcg aaaaattcag
     3001 ggaacaagat ccactgcaaa gaaaacagaa acgcaaaaag cggacatgac agccgaagga
     3061 aaagaagtca aagaaatatc caccctacag gatgttcttt aaataactaa agttggcgtt
     3121 cgtcgctggg atttgcaatc ccagcttctt gtattataag gatttataat ccgctaagtt
     3181 aaatatttag attaaaaagt tatctttgct tcaataaata tattttgccg tggaaagtgt
     3241 ttcggattac aaacctttat aatacattac gaggtgattg caattattct cttctgtcat
     3301 gaccgttggt tcacctcaga cgtccgcaaa gctttgtata ttgaatgagg atattcagta
     3361 ggacggggtt atctcccgca ttatcttttg tgttgttcga cagtgaatac gcccaccatt
     3421 cggctattgc gtttagcctc ggtcgatatc aagaaactta tcattcgata tcaagcataa
     3481 actcgaaaat gatttacgca ggtttgatat tttgtattta agaatattgt atatttgttg
     3541 cgtaaatgag acgttacctg taattttttt aacttaaaac aaatatttat gctcaaacac
     3601 agatattaca ttatcaaaca caaatatgct acccgaagtt tcaaatgcag ttaacacatt
     3661 aaatacttca ggttcggtta ttgttaagtc aaccgataga attacatttg aagctgggca
     3721 agaaataata ttgaataccg gatttgaagt tcaacaagga ggtcaacttg atgtaaatat
     3781 tgtaacatgt ggtcaataat ctaaatctaa aaaaacatga aaagaattct tattgtatta
     3841 gtctttaacc tattgttttc acattttctt tttgctcagg aaaacagtag aataaactca
     3901 tttttcaacg atactttgaa gggaaaatgg attttagaaa gtaattgcct tttagaatat
     3961 caaagatgtg attctattgg aaaaggaatt gagtatataa tattcaattc aataaattca
     4021 cagggttttg taaaagttgt aacaaatggt tcttggagtt ttgagaatgg agattctaca
     4081 aaattatatc ttcctgaaaa aacaattttt ggcgaaacat gggtcatgga cgattatatt
     4141 ttttattctt ataacaaaat atgtgttata aatgagaata ttgacaaatt attcctatct
     4201 actaacagtt ttattggatt tgcaaaatca tatatccgtg acaaatctta tttagggttg
     4261 caatctaaaa taaatatacc aataacaatt tatcccaatc catttatcga taagattaat
     4321 gtttcttatg atatcccaaa ttcaacgaat agagtattga taaacatata ttcctctaca
     4381 ggattattac taaaaaaaat cgaaattgca gaaaatggac agtttaagaa aacaatctca
     4441 ttggaggatt taaataaagg aatttatttt ggagttgttt ctctaaatga taaatggatt
     4501 aacaacataa aactaattaa aaagtaaatt caaaaaaatc aaataagaca aaacgaacaa
     4561 gaaatagaaa caattttcaa actttggtaa cacacgccta agcacaatag ccgaattcgt
     4621 gcgattttgg aggtttatct ctcgcataag tttttgtgtt gttcgacagt gaatatgcct
     4681 ctgctgccgc acgattgcat cgtgtggcag cgtggggttt ggtgagaaga aaaatttgta
     4741 actgaaaaag aaaagaatga aaatttatcg atttccgaat gattttttta agtattgaat
     4801 tgattgatcg attaatattc tttgtaactt tcactgcgaa gtacgcgcaa agctcccatg
     4861 gccaatgctt ccacttcgtt ttcgcccgga tatacgacta attttccgaa tgaggaaacc
     4921 atttctttca cgtaatttac aaaccattca cttctactga ttcctcccgt tagaattatg
     4981 gcatcaactt ttccttttaa aacggtgcac atagctccaa tttcctttgc aacattatat
     5041 gccatagctt ccattatcag tttggcatgg gtatctccat tcacagcttt ttgttcggcc
     5101 aaataagcat cattggttcc aagatgtgct atcagtccac ctcttccgac cagcattttt
     5161 tttatttctt ctttggaagc ttgaccttga aaacaaaagt ccaccagcaa tccggcatca
     5221 atcgttcctg atctttctgg cgaaaacggc ccataaccgc ccaagccttg attggtgtct
     5281 atcacttttc cttttgcatg tgccgaaaca ctgattcctc cacccaaatg agcaattacc
     5341 aaattcaaat tttcgtaacc ggcgcttact gaagcagcat attttcttgc tacagctttt
     5401 tggttgagcg catgaaacat ggaacgacga ggaaattgag gcaatccact gatgcgggca
     5461 acatcctgca tctcgtccac caccacggga tcaacgataa agggcttgca ttctggaata
     5521 tcgcgggata tttcataggc aatgatgccg cccaaattac ttgcatgttc tccttttttg
     5581 gcctctctca aatcggcaat cattttttcg ttcaccaaat atactcctga agctaccggt
     5641 cgaatcagtc ctcctctgcc aacaatagcg gaaagtgttt gtaaatctac accatcatgc
     5701 ttcagactgt taagaattaa attttttctg aaatcgagct gatcaattat tttttcaaac
     5761 gattttaatt cttccgttga atgtaaaatg gtatgaagaa ataaaatttt ttcgtcctca
     5821 tagacggcaa attttgtaga agtagaaccg ggattgattg aaaggatttt aaacatgggt
     5881 gtaggatttg tgtcaacatt tttatgcaaa tttatgatat tttctgacat attctaaata
     5941 tttgatttct ttaaaggaaa aatctttgtt ttaagtaatg tggttgcaat accttaaata
     6001 attcataatc agtcataaag ttataattgg ttgttttatt gtaaaaagag aaaatgttta
     6061 tttgataaaa ccttggattt tatataatta ttttgtaatt ttgcaacgtg aaaagaaatt
     6121 gtgagagggg acaaaattgt ccccttgttt tttagataaa tgtgtacttt ccgatgattg
     6181 aacagacaac catattgcaa atagtagaaa attttgtttc aggcacagat ttttacattg
     6241 tcgaagttaa agtgagccct gaaaatcaga ttcttgtcgt ttttgattca tttgaaggta
     6301 tttccattga cgattgcgaa gctttgaaca atttcatcga atccaaactg gacagagaac
     6361 aggaagatta tgaattggaa gtcagctctc ccggactgac agaacccttt aaagtactga
     6421 aacagtacga aaaaaacaaa ggtaatgaag tggaaattgt tacaaaaaaa ggagaaaaat
     6481 tttccggaat actcacagag gtaaattttg ataattgtac gcttcaaatt gaaaaattaa
     6541 tcaaacccga aggtgcaaaa aggaaaatca aagttacaga aaaagttcct tttcgatatg
     6601 aagatattaa aactacaaaa tatattttaa gatttaaata agccatgagc aaaaaagaaa
     6661 ctgttaattt aattgatact ttttcagaat ttaaagagct taaaaacatt gatcgaagta
     6721 ctttaatcag tgtaatggaa gaatcgttcc gcaatattat agcaaaaaat tatggaaccg
     6781 ataagaattt tgacgtcatc atcaaccccg ataagggaga ttttgaaata tatcgaaacc
     6841 gcactgtagt ggcagatgat gaggtaagcg atccaaattt agaaatatcg ctcagtgaag
     6901 cccaaaaaat tgatgaagat tatcaaatag gagaagaagt taccgatacc gttgacattt
     6961 cggatttcgg tcgacgtgcc atcttgactt tgcggcaaac gctgcaatca aagattctgg
     7021 agttgcaaaa ggataacgtt tataccaaat acagcaaaat ggttggtcaa attgttacgg
     7081 ccgaactata tcagatttgg aaaaaagaaa tgcttctcat ggatgaagaa ggaaatgagc
     7141 tttatttgcc aaaatcagaa cagattccga cggattttta tcgaaaaggc gacatggtga
     7201 aagctgtagt ggctatggtg gaaaataaaa acaacaatcc gcgagtcatt ctttcccgta
     7261 tttcgccggt atttttggaa cgactttttg aaaatgaaat tcctgaaata catgatggac
     7321 tgataaccat aaaaaaaata gccagaattc ccggcgaacg cgctaaagtt gctgttgaat
     7381 cctatgacga ccgcattgac cctgttggag cttgcgtagg tatgaagggt tcaagaattc
     7441 acggaattgt gcgtgaattg cgaaatgaaa atatagatgt catcaattat acgaataaca
     7501 tcagtttgtt catttccaga gcattgagtc cggccaaaat ttcatctatc cgcatcaatg
     7561 aagctgaaaa gaaagccgaa atttatctga aacccgaaga agtttctcaa gccatcggaa
     7621 aaggagggct caatattaag cttgccagca tgcttaccgg ttatacactc gatgttttcc
     7681 gcgaattgga tgaaaccgaa acagaagata tttacttgga tgaattcaat gacgaaatcg
     7741 atcagtgggt tattgatacc ttaaaatcta tcggttgcga tacggctaaa aatgtattgg
     7801 ccattccacg tgaagatttg attcgtcgca ccgatttgga agaagaaact attgatgaag
     7861 tgctggacat tttacgttcg gaatttgaag acgaaaattg agaatttttt caataaatga
     7921 acaaaagttc aaagctttta aagttaataa ttgtaaaata aatatatgcc tataagatta
     7981 agtaaagcat gcaaggattt gaatgtcggt atgagtacgg ccgtcgaatt tcttgcaaaa
     8041 aaaggtcata aattgtcagc cgatccgaat ttaaaattgg aagatgagct gtatctttta
     8101 ctcgcaaaag aatttaataa ggatatggcg ttaaaaattg aatcggaacg gatcagcaaa
     8161 gaacggcagg aaaaagaaaa agccgaaact gtcggattgg aagagtatgt tcatcccaaa
     8221 acggcaccaa aacctgccga aataatacct actgtcatcc ctgaagaaca aaagcctcat
     8281 atcaaatctg tgggacacat tgacttggaa gaaacaggga aaaaagttaa aacgaaagaa
     8341 aaagaaaaaa cggaaattgt tgactcgatt cctgttgaac caataaagga acctgttgtt
     8401 gccgaaaaag aaaaaattga acccgatgct actttcactc atgaagtaaa agcggttgtt
     8461 accgaagaac cagtaaaacc ggaaaaacct gaagaaataa ttcaggaaca aaaatctcaa
     8521 cctgaggaat ttgaagttac agagaaaatt ttgcccgatt caacagaaat taaggccgag
     8581 aaaatagaag aaaaaattga aactcaagta ataacttctc ccgaaatttc caagcatcgg
     8641 acaactcagg aatcagaatc ttccaatttg gaagaagtta atgttaatga agcagaaatt
     8701 gaaatttcgg aactaacgga agaagttttt tacaccgaaa tacctgtttt ggacaccaat
     8761 attactgtgg tgggaagcat cgaccttgat gcactcaacc agagggttag accaaaacca
     8821 aaatcgaagg aacagagaaa gagggaaaaa gaagaaaaaa ttcagttaga agctgaagca
     8881 aaaaaaattg ctcaaaaggg taaaaaagtt attaaacttc ccggtgaagc agaagatgcg
     8941 gaagatacaa aagatcatca caaaaagaaa cgcgaacgtt ttacaaagca aaaagttgat
     9001 atttctaaag tttccggcaa tgccaatccg gaggaattgg aacgtccaaa aaaattaaat
     9061 ctttctccca aaggaaccaa agataaattt aaaaaatcca tcaaacctgc catttcggaa
     9121 gaagacatac agaaacaaat caaggaaacc cttgctcgtt tggcaacttc caataaaaag
     9181 acaaagggtt caaaatacaa aaaagacaaa agagaaatta tcaatgcccg tcagcaacag
     9241 ctggcagaaa tggagcagag caacgaaaaa gtactgaaac tgacggaatt cgtaactgtg
     9301 aatgaactgg ctaacatgat ggatgttccg gtcaacgatg tgatttctac ctgtatgaat
     9361 cttggtttga tcgtttccat caatcaaagg ttggatgctg aaactattaa tattttagcc
     9421 gatgaatttg gcttcactac cgaatatgtc agccatgatg tgatagaagc acttgctgaa
     9481 aacgaacccg acgatgaaga aaatcttcaa cctcgtccac ctattgtaac ggtaatggga
     9541 catgtcgatc atggtaaaac atctttgctg gactacatcc gcaaaacaaa tgtgatagcc
     9601 ggagaagccg gaggaattac ccagcacatt ggcgcttaca atgtggtgtt ggataatggg
     9661 cagcgaatta cttttctcga cacaccgggt catgaagcgt ttacggccat gcgtgccaga
     9721 ggtgcaaaag ttaccgacct ggccatcatt attattgctg ccgacgataa cgtgatgcct
     9781 cagactgtgg aagccataaa tcatgcttcg gcagccaatg tccccattgt atttgccatc
     9841 aacaaagtgg ataagcccga tgccaatccc gaaaaaataa aagaacaatt ggccaatatg
     9901 aattatttgg tggaagattg gggcggtaaa tatcaatcgc aggaaatttc agcaaaaaaa
     9961 ggaatcggag tgcccgaatt gcttgagaag gtattgctcg aagccgaatt gctcgatttg
    10021 aaagccaatc ccaacagaag agcaataggt tctattattg aatcatcgtt ggataaaggt
    10081 agaggatata ttgctacggt tttggtacaa aatggaacgc tccatcaagg cgacgtagtt
    10141 ttggcaggaa cacatttcgg aaaagtaaaa gccatgttta atgaaagaaa tcaaagaatc
    10201 tctgaagcac gaccttcgga acctgttttg attttaggat tgaacggagc acctcaggcc
    10261 ggagataatt ttgtggtaat ggaatcggaa catgaagcgc gtgaattggc attgaaacgt
    10321 gaacagttgc aaagagaaca aacaattcgt actaaaaaac attttacact ggacgaattg
    10381 ggaagaagaa ttgcattggg cgattttaag gaattgaatg tcattgtaaa aggtgatgtt
    10441 gacgggtcag ttgaagctat ttccgactcg ctgttaaaac tttctaccga aaaaattcag
    10501 gtaaatatta ttcataaggc tgtgggtgcc atttcggatt ccgatgtgtt gctggcagca
    10561 gcttccaatg ccatcattgt gggttttcag gtacgtccta ctccttctgc ccgaaaaatg
    10621 gcagaaaagg aagaaatcga tattcgactc tattccatca tatatgatgc cattgaagaa
    10681 attaaatcgg caatggaagg catgctctcg cctgaaatta aggaagagat tacggctact
    10741 cttgaagtca gagatgtgtt taaaattaca aaagtgggaa ctgtagccgg ttgtatagtg
    10801 aaagaaggaa aaataaaacg aaccaacaaa gttcggttga ttcgcgatgg tattgttatt
    10861 tttaccggcg aaatcgaatc gttaaaaaga tttaaagagg atgtaaaaga agttgctgtc
    10921 aactatgaat gcggtgtaag tattcacaat ttcaacgata ttcatgtagg agatttgata
    10981 gaaagttttg aagaaatgga agtgaaatcg gaactgtaag ccggcaatat ttctctttaa
    11041 tgctgtcgaa cactcagaca agaatcatga aaattttgaa aaagtcgcca tgagttagag
    11101 accgaaatta ataagatacc gcaggtaagc cgatgaaatt ataggacaaa caaaaaattc
    11161 gaaacgaaga tagtttcaaa aatagcagtc ttttcctgca attgtcataa aatcaattga
    11221 tgaaaaatct gcattgaact gaaaaatttt ccggaagatt tgattttttt attggaacaa
    11281 actcttatta aaccaaatac ggattaaaat tcttttcgtt tccaatggta gtgggttgtc
    11341 cgtgtccgct gtagaccaat gtttcatcgg gtagaaccat cagcttttcc caaatggaac
    11401 ggattaataa gtcgtgatcg cctttttcaa aatgggtggc tccaattgtg ccgtagaaca
    11461 agacatctcc ggtaaatacc aaaccatttt gttctgaata aaaagccatg cttccgggtg
    11521 aatggcccgg aacatgtaat gctttcaaaa ttgagtttcc aaattttatt tcctgatttt
    11581 caataatata agagccaatt gcaggaggtt cttcatgcaa gtcgatacca aaaaattttg
    11641 ctgcttcatg catggtttcg attaaaaatt catcttcctt gcatgcttca ggcgaaatgt
    11701 gatagttgtc gtaaatataa cgatttccaa attggtggtc gatatgcaaa tgggtattga
    11761 gtaaatgttt taaaacaagc ttattatttt caatataatt cttgacagct tgtttttcgc
    11821 ggtttgtcag acaacccgca tcaataatga tggcttcacc agttacatcc gaaacaatat
    11881 aggtgttttc tctgaaagga ttgaaaataa aggtttttat gttcattttt atattctgta
    11941 aaggttttgc ctgattaatt tttttcacac aacaggcaaa taaacaattt ttttgtcttt
    12001 aaaatattct tctttaaaaa aatcggaaat actggctact tcaattatct ttttgaaagg
    12061 cttaatttct tgttccaaac taccaccctt taaagaaata attccattgg gtaacgcatt
    12121 gatggaggtt ttccgaatat tttttttggt cattttcagt aaatcttcta gtggcatgac
    12181 tgcacgactg acaataaaat cgtattttcc cttttcttct tctgctcgta aatatttgaa
    12241 ttcggaattt ttcaactcaa tggcctcagc aaccgacatt gcaactttaa ttttttttcc
    12301 tatggaatct atcattacaa atttaacttc gggaaaaagt atagcaagcg gaataccggg
    12361 aaatcctcct ccggtaccca catccaaaat acttgagttc ggcttgaaac ggatatattg
    12421 agcaatagca agagaatgaa gaacatgatg ttcgtacagg ttttgaatat cttttctcga
    12481 aatcacattt attttttcat tccaatcctt ataaagggga tacagcgctt caaactgagc
    12541 tttctgatga gatgacaagt cgggaaaata tttattgatc aattccataa actcattttt
    12601 tggcatacaa agttaagcgt ttttacaaaa gtataaaatt tcatttgatc gattcttaat
    12661 attcaaggca aaaaaccaat tgaaaaaata aataagctca tctgacaata tattaacaaa
    12721 aaggcttcat tgaaaggatc aggatatcaa aatatttaga aaaatcttaa ccgaatacct
    12781 cgagataaat tgaattttaa ttggctttaa aaaagagttt ttcgaacatt ttttaatttc
    12841 gaacttgccg gttgactcta ctaatttcta atttttttgt aatgttatat ttcttttgta
    12901 attttgatta actttgttga gtgttttaaa aagtagattt tagaatgaat catattccga
    12961 agttatccca tatacttgtt ctgattttgg ttgtacaact ttcttcgtgt attactgccc
    13021 gcaaaattaa ttacctgcag cagcccaatg cttccattcc tgcgtataag gatacttttt
    13081 cattcaacga ttacagactg aaaaaggggg acagggtttt tataaaaatc tattcatggg
    13141 atgataaaac aaatgcattg ctaaatggta gcagcaatat agaacagctt gtcatgtcgg
    13201 gttcttcatc cggcaacgaa ctgtacactt atctgattca gccctccgga aaagtaaatt
    13261 tgccaatggt aggagaaata tatttggaag gtcaaactgt aagagaagcc aaagaaacgc
    13321 tcgaaaaatt tatacaaccg gtttttgaca tcagcagcgt cgatgttaga attgctggaa
    13381 gatttttcag tgttattggt ggaggtgcaa ccggcaggta tcctttaaat cgggaaaaaa
    13441 taaatatttt tgaagcgctg gcaatggccg gcgacattga tacttatggc gatagaagta
    13501 aaataaggat tatcagagaa acaaacagtg gaactcagat aaaggttttt gatgtgcgaa
    13561 gtgtggatat tatcaattcc gaattttatt acgttgagcc caatgatgtg atttacattc
    13621 aggacatgaa cgaacagttt ttcagtgtaa cgtcctttgc ttcacttttg tcgacggttt
    13681 ttgctacttt ttcatttgga atttttattt atggtttgac ccgttaatcg gtttgaagag
    13741 agatgaaaaa attatgaaac attttcttca tataattttt acaattttga ctgttttgtc
    13801 cttttcttcc tgttacaatt acagaagcat tgggttactg caggaaaaca aatcgctacc
    13861 cgtttattct aaggcagatt attcggatta tgaaatttgt gttaacgacg aaattatttt
    13921 tcgtttgatc actacggatg aaaccatttc aaagttgatt tcttccaatc agtcaggcaa
    13981 tagcaatcag atgatttctt accgcgttta tccggatggt actgtggaca ttcccttttt
    14041 aaatcgcgtt caggtgaaag gattgaccat aaatgaagct gagaatttgt tgaaggaaga
    14101 attcagggaa attattcctg atgctgaagt gaaactgagt attgccaata aatcatttac
    14161 agtaattgga gaagcaggga ctggcgtttt ccctgtatat aaggataaac ttactattta
    14221 tcaggctttg tccctgtcag gtgatttgaa actttccggt gatttccggc atgtgaaaat
    14281 tttacgacca gtggagggaa aaaccgagat attggagttt gatattcgtc cggcaagtgt
    14341 aattgaatcg aaatactatt atgtttaccc caacgatatc atttatgtgc agcgggcatc
    14401 gtcgagcttt tttaaaatga acaattattc ttcttttctt ggactgattt cgtcttcttt
    14461 aactttattg ttcagcgttt tatattatat caaattgtaa attcacagca taaacctatc
    14521 ttttattcta agatatgaat ccggatacag gcagcattaa ccagaacttt accgaagaag
    14581 aatcttcctt tgattttcag aagtggcttc atttatttat caaaaattgg tatttgtttt
    14641 taatatttat tccgtttttt ttaattggag gatatttaaa aaacagaagt tggatgcctc
    14701 tatatcagag tgttggaaca attattatta atgaaaacaa ataccgatcc tcttcacaag
    14761 gtttcatgca gggatttggg ttggaagaag cttaccagaa tgtcaataat caggtgataa
    14821 ttctcaattc atacgatttg attggcaaag ttgtagatag tttacctttt atgaaagtgg
    14881 actatttgac gattggtcgt ttcaaaaccc gtaatcttta tactacaact cctattatta
    14941 ttgaagccga ttatgtggct ccggaagctt acgaactgct ttttaaaatt tcacttcatc
    15001 cgaacggcac ttttaccatc agcccacata ataacaatag catgccaagt tcaccaatca
    15061 atggtcatat aggcgtgcca cttcagcata atttattttt tatgactgta aacagcgtga
    15121 gcgacaatgt taaggaattt gaaatgtatt ttcgctttcg taccagagaa acattgattg
    15181 aagattttaa atcgcgatta agttcaagat acgtcattga aggttcttcc attttggaaa
    15241 tttcattgat cagtgaaact ccagacaggg atgtggactt tatcaacaaa ttgagcgaaa
    15301 tctaccttga agaaaatttg aaaaagaaaa acgatgttgc ctccaaaacc attcaattta
    15361 ttaatgaaca attatcggag gtatccaaat ccctgtctgt ttctgaagat gaaatgacac
    15421 gattcagaag ggataatcaa atcattgata tttcgagtca tgggagtgaa ttgatgagca
    15481 aggccaattc tttggatgaa aagaaagccg aactaaaact caaggaaact tatctgaatt
    15541 atttga
//
LOCUS       DAOSIB010000052        15532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_3957_length_15532_cov_10.563869, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000052 DAOSIB010000000
VERSION     DAOSIB010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15532
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3957_length_15532_cov_10.563869"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="PLD44_05870"
     CDS             <1..1095
                     /locus_tag="PLD44_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOF98896.1"
                     /translation="SLNYYPIGNEKPEDGIANIAMGLGKYIVDGGKTLRFSPAYPNNV
                     LQTSTLEFMLSETQTFFNALDLNMVQFVPQVDDGFNLLRINVQDAIKDGTLQYLASTY
                     NVSDQMVYDGLYEGGRKIITFANILQHNVFPLAELLREILRIAQEGMGRPVEIEFAVN
                     LDYSAKKQHSFYLLQVRPIVDNKEIINEDISAIPEEETIVHSSNALGNGNMTDIYDLI
                     YVKQEAFNGSNNRLIMYDIEKLNRQMVKEEKYYVLIGPGRWGSSDPWLGIPVKWPNIS
                     NARVIVETELSHYRIDPSQGTHFFQNLTSFGVAYFTVQPFQHLGFYDLDFLAQQPAIY
                     ESEYIRHLHFDKPLVVKVDGKKNIGVIMKP"
     gene            complement(1231..1677)
                     /locus_tag="PLD44_05875"
     CDS             complement(1231..1677)
                     /locus_tag="PLD44_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOF98897.1"
                     /translation="MEPIILAYIGIGLMVGLSGVGSAIGVTITGNATVGALKKNPDAF
                     GNYMVLSALPGTQGLYGFLGYFLLSSHLVPNMTWGASAAVFGASLALALTGLLSAIRQ
                     GQVCANGIAGIGSGYDVFGRTLILAVFPELYAIVSLAAVFMIGNAI"
     gene            complement(1746..3569)
                     /locus_tag="PLD44_05880"
     CDS             complement(1746..3569)
                     /locus_tag="PLD44_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOF98898.1"
                     /translation="MTRYTFLVYHKQYHDFLEKMREVGVVHVIEKVEGIAENDELREK
                     MLLSSRMKIALKQMEKLIPQDASPLPADLSVDGMELLKKIENKFAEKELLIQKLQLAE
                     KERDRMEMWGIFDYQLIENLQKAGYEINFFSCNVRKFDQEWEIRYNAFEIDNIGSTRY
                     FVTITHTGEIIEIDADPVKIGTKNVLQIDEDIQQIHQEIDNINRQLITLSVENYNSLK
                     AAQVKVIENMDFARVLLNTEPTVENKVMLLEGWCPEDAEEKLKEFLNETDIYYETSKP
                     EEDEKVPVKLKNNRFSRLFEFIGNLYDLPNYHELDLTPFFAPFYMLFFGICFGDAAYG
                     LLLLIASLYFRKKADAALKPILSLAALLGISTVIMGFVSGTFFGFNLLEMDVPWLASF
                     KAFMLDSNQLFYTSLLLGVVQILFGMVIKAIGKVKRYGWPASFSTWGWLILLIGCGGT
                     FVLSSFAGLSPQTAKWLYIIFGSAGGALVFLLNNIKRNPLINIGSGLWDTYNTATGLL
                     GDVLSYIRLFALGISGAVMGFVFNDLAINLSGNIPIVSTLIMLVIMIFGHGINIFMCG
                     LGAFVHPMRLTFVEFYKNAGFEGGGKKYKPFSHFKEEKSII"
     gene            complement(3578..4183)
                     /locus_tag="PLD44_05885"
     CDS             complement(3578..4183)
                     /locus_tag="PLD44_05885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOF98899.1"
                     /translation="MSIQFQYNKTSLQQLEKNLKIRVRALPTIKNKESALRTEVKKAK
                     EEIKRIENELEQRIAEYDKMLALWGEFDTTLLEVEEVKMNIKKIAAVRVPILEEVVYK
                     TKPFSLFNSPKWYSDGLALLKKLAQIGIEQEFFIQKLSLLEFARKKTTQKVNLFEKVQ
                     IPGYEDAIRKIKRFMEDEENLSKSSQKIVKSRQELIKKENL"
     gene            complement(4239..5570)
                     /locus_tag="PLD44_05890"
     CDS             complement(4239..5570)
                     /locus_tag="PLD44_05890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOF98900.1"
                     /translation="MTTKAFQKIYTKITKITKATCSLHASGIGNDELALVNGKLAQVV
                     KILGDEVTLQVFEGTEGIPTDAEIVFLGKAPTLKVSDQLAGRFFNAFGDPIDDRPEIE
                     GEEREIGGPSVNPVRRKQPSELIATGIAGIDLNNTLVSGQKIPFFADPDQPYNEVMAN
                     VALRAKTDKIILGGMGLTNDDYLYFKNVFNNAGVLDRIVSFVNTTEDPPVERLLIPDM
                     ALTAAEYFAVDKNEKVLVLLTDMTLYADALAIVSNRMDQIPSKDSMPGSLYSDLAKIY
                     EKAVQFPAGGSITIIAVTTLSGGDITHAIPDNTGYITEGQLFLRRDSDIGKVIVDPFR
                     SLSRLKQLVIGIKTREDHPQVMNAAVRLYADAANAKTKLENGFDLTDYDERALAFAKD
                     YSDRLLAIDVNIDTTAMLDTAWKLFAKYFSPTEVNIKQEFIDKYWKQKQHE"
     gene            complement(5645..7405)
                     /locus_tag="PLD44_05895"
     CDS             complement(5645..7405)
                     /locus_tag="PLD44_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOF98901.1"
                     /translation="MPTKGKVKGIVSNLVTVSVDGAVSQNEICFIELGSEKLMAEVLK
                     VNGSDVFVQVFESTRGLKIDNEVTFEGHMLEVILGPGLLSKNFDGLQNDLNKLTGIFL
                     KRGERNFPLDEDKKWNFKPIAQVGDEVEAASWLGEVDENHQPHKIMVPFVMEGSYKVK
                     SIAPAGEYTISETIAIITDAHGKDFEINMIQKWPVKKAINVHAEKPRPYKLLETGVRV
                     IDTANPIVEGGTGFIPGAFGTGKTVLQHAISKQAEADIVIMAACGERANEVVELFVEF
                     PELKDPHTGRFLMERTIIVANTSNMPVAAREASVYTAMTIAEYYRAMGLRVLLMADST
                     SRWAQALREMSNRMEELPGQDAFPMDLSAIIANFYSRSGFVKLKNKQTGSITFIGTVS
                     PAGGNLKEPVTESTKKVARCYFGLEQARADSKRYPAINPIESYSKYIEYPEFDEYISE
                     RISPQWISMVNDMRTLLQRGKEVQEQINILGDDGVPVDYHITFWKSELIDFVILQQDA
                     FDDIDAVTPIERQHYMLKLIMDICKSDFNFVNFTDVMDYFKRIINVCKQMNYSEFHSQ
                     AFESYHNELQNILAERKIKQ"
     gene            complement(7492..8349)
                     /locus_tag="PLD44_05900"
     CDS             complement(7492..8349)
                     /locus_tag="PLD44_05900"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOF98902.1"
                     /translation="MNYYCLIAGLPDLSPDDKKTTFTLADFKNELLELLDTSDVEFLR
                     LIFAQYDNQNFLAFLIDRDAFLQPLGNLNYNDWVEIVQLLKETENPKDKRILPYIQHF
                     YSVYQDEEFWAEGISKEDYLTGLYYDFALQCNNSFLKSWFEFSLNINNLLTAIACRKY
                     GLNPHNYIVGSNTIAQQLRTSNARDYGLTGTFEYLEQVLQIAEEPDLMEREKKIDLLK
                     WQWMDEHTFFYYFTIEKILAYILKMQMIERWQPLTPEKGNVIFRELIEKMKQSVEFEE
                     TLQAGEKIR"
     gene            complement(8428..9018)
                     /locus_tag="PLD44_05905"
     CDS             complement(8428..9018)
                     /locus_tag="PLD44_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98903.1"
                     /translation="MNTKLQELTEKIYLEGVEKGNAEAQSIIEEAQKKADSIISSAQE
                     KAARIIAEAEAKAAELEENTKSELRLYAQQSLNALKTEITNLICGKIVDDSVKAAMVD
                     KNFMQQVILQLAKEWSKKESIVIEAPDAKALTEYFLANAKNLLNQGVTITQADRSKAT
                     FTIRPDKGGYKINFGEEELTEYFKDFLRPKLIEMLF"
     gene            complement(9410..10078)
                     /locus_tag="PLD44_05910"
     CDS             complement(9410..10078)
                     /locus_tag="PLD44_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98904.1"
                     /translation="MKKTIKLAIAVSILTFISCKDNTPNQPYQYELNPAYTWGYVVFY
                     GDYYADYLNENNVLTVNLFTDSLKINNQNELSGYGQYLFLEDVFVAPSDTLLPEGIYR
                     VDNSGEPFTIAPGINDTIDNQVYTLGAVINYYEPNPLKSTQKLIVGGKMTVSRQGENY
                     SLNCDFVLQDSLKLKGIFSGTLPHFDESLQREERNSIYIFKENSLVKEKKTIYKFFGK
                     KKNY"
     gene            complement(10160..11449)
                     /locus_tag="PLD44_05915"
     CDS             complement(10160..11449)
                     /locus_tag="PLD44_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF98905.1"
                     /translation="METIHPNQTKRDNTSGGFRFALWVARRYLFSRKSHNAINIISGI
                     AALGVGIGTMALICVLSVFNGFDSLIQGMFSAFDPDLKITVSQGKTFDINTPEWEKVK
                     KLNDVAYFTEVIEENALLRFRDKQMPAIIKGVSDDFQKMTKIDSIMFDGKFLLYDGAF
                     QRAVPGVGVASILGLGANFIDPLYIYAPKRTSHINLLRPENSFNQIGTFVSGIFSVKQ
                     LQYDDHYVLVSIELARDLFEYQPNKVSSIELKLKDNIKTLDAQKKIKNILGGNFEVKD
                     RYEQQEDFFKILKIEKWITYLILCFILLIASFNIISSLSMLIIDKKQDIQTLRNLGAE
                     QQLIRRIFLFEGWLISAVGAVLGIIMGLLLCLLQQHFGILKLGTGYVVDVYPVLVNFW
                     DVLLVFMTVLLMGFLAAYYPVRYIDIHDAPSPVNVEL"
     gene            complement(11495..11875)
                     /gene="rbfA"
                     /locus_tag="PLD44_05920"
     CDS             complement(11495..11875)
                     /gene="rbfA"
                     /locus_tag="PLD44_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOF98906.1"
                     /translation="MDNTRQQKVARLLQKELGEIFLLYAREMHGIIISVTNVRISPDL
                     SIARIHLSIFPGKQSAEVLEKINNDQKNIRFDLGKRVKHQLRIIPELFFYLDDSLDYI
                     DHIDQLLKNDSPSDKKDHSQENNQ"
     assembly_gap    12055..12154
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12155..12988)
                     /gene="rhuM"
                     /locus_tag="PLD44_05925"
                     /pseudo
     CDS             complement(12155..12988)
                     /gene="rhuM"
                     /locus_tag="PLD44_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201977.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            complement(13021..14487)
                     /gene="cysS"
                     /locus_tag="PLD44_05930"
     CDS             complement(13021..14487)
                     /gene="cysS"
                     /locus_tag="PLD44_05930"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOF98907.1"
                     /translation="MKNNLYIYNTLHRKKELFVPLHAPHVGMYVCGPTVYGDPHLGHA
                     RPAITFDVLFRYLKHLGYKVRYVRNITDVGHLENDADSGEDKIEKKARLEQLEPMEIA
                     QYYANRYHKAMEALNVLPPSIEPHASGHIPEQIEFVKKILDAGFAYESEGSVYFDVEK
                     YDKKYHYGKLSGRNIDELLETTRMLEGQEEKRRSVDFALWKKASPEHIMRWKSPWSEG
                     FPGWHLECSAMSTKYLGEEFDIHGGGMDLLFPHHECEISQSTAALGKESVRYWMHNNM
                     ITINGQKMGKSLGNFITLEEFFSGNHPLLSQAYQPMTVRFFILQAHYRSTVDFSNEAL
                     QASEKGLERLMEGYDRLQKLMPSDISTSDISGLRQRCEEAMCDDLNTPIVIAHLFEAV
                     KFINLVYDGKATVSSADLEELKDVFRVFVEDLLGLKKEDENFAKYEALKKAVDLLLQI
                     RLEAKRNKDWATSDKIRNELTALGFEIKDTKDGFEWKI"
     gene            complement(14532..15266)
                     /locus_tag="PLD44_05935"
     CDS             complement(14532..15266)
                     /locus_tag="PLD44_05935"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOF98908.1"
                     /translation="MSSFAKWIGGGLGWALGGPIGALIGFAIGSFIGAKLETVEKSQN
                     RRTTTTSGDFKISLLVLIACMMKADGHPKKSELEIVKRFLVNNFGEEGALEALSILKN
                     LLEKDIDERQVAEQIYRYTNYATRLELIHLLFDIAYADHQVSTSELTLIERIAGIFRI
                     SRLDFESLKAPYIKQVDINWAYKALEIEPSASVDEIKKAYRKMAMKYHPDKVNTLGED
                     VKKKATEKFRSINEAYETLKMERKFT"
BASE COUNT         4521 a         3004 c         2722 g         5185 t
ORIGIN      
        1 tcgttgaact attatccgat tggaaatgaa aagcctgaag atggaattgc caatattgca
       61 atgggattgg gaaaatacat tgtcgatgga ggcaaaaccc ttcgattttc tcccgcctat
      121 cccaataatg ttttgcaaac cagtacgctg gaatttatgt taagcgaaac acagacgttt
      181 ttcaacgcat tggatttgaa tatggtacag ttcgttcctc aggttgatga tggcttcaac
      241 cttttgcgta tcaacgtgca ggatgccata aaagacggga cgcttcaata tctggcttca
      301 acctacaatg tgtcggatca gatggtgtat gatggcttat atgaaggtgg aagaaaaatc
      361 ataacttttg ccaacatatt gcaacacaat gtttttcctt tggccgaatt gttgcgagaa
      421 attttgcgca ttgcgcagga aggcatgggg cgaccggttg aaattgaatt tgccgtaaat
      481 ttggattatt cagccaaaaa gcaacattcg ttttatttgt tgcaggttag gccgatagtc
      541 gacaacaagg aaattatcaa tgaagatatc agtgctattc ccgaagaaga aactattgtt
      601 cacagttcca atgctttggg caacggcaat atgaccgata tttacgattt gatttacgta
      661 aaacaggaag ctttcaatgg ctcaaataat cggttgatca tgtacgacat tgagaaattg
      721 aaccggcaaa tggttaaaga agaaaagtat tatgtgttga ttgggcctgg ccgatggggc
      781 tcaagcgacc cttggctggg cattccggtg aaatggccca acattagcaa tgccagagta
      841 attgtggaaa cggaattatc gcattatcga atcgacccca gtcagggaac acattttttc
      901 caaaatctga cttcttttgg tgtagcctat tttacggtac aaccttttca gcatcttggt
      961 ttttatgacc tcgatttctt ggctcagcaa ccggcaatat acgaatcgga atacattcgt
     1021 catttgcatt tcgataagcc gctggtggtg aaagtggatg ggaaaaagaa tataggcgtg
     1081 atcatgaaac cataaaattt tttctgaaat gctaaaccta atgaaaacat cgaacaagac
     1141 aaaaatcctg ttcgatgttc acaatgagaa aaaataattg atttgaggtt gttttattgt
     1201 gtttcagaga tatttttgca gattggaata tcagatagca tttccaatca taaaaacagc
     1261 tgccaatgaa acaatggcat aaagttcagg aaatactgcc agtattaatg ttcgaccaaa
     1321 gacgtcatat cctgatccaa taccggctat accatttgca caaacttggc cttgtcgtat
     1381 ggcagaaaga agccctgtga gggcaagtgc caaactggcg ccaaatactg ctgctgaagc
     1441 accccacgtc atgttgggaa ctaaatgtga tgagagcaaa aaatatccaa gaaatccata
     1501 taatccttgt gtaccgggta atgcacttaa aaccatatag tttccaaatg catcgggatt
     1561 ttttttcaaa gcaccgacag tggcattgcc ggtaatcgtt acgccaatag cacttcctac
     1621 tcctgaaagt cccaccatta ggccaatacc aatgtaagct aaaataatag gttccataat
     1681 ttttttttta aatttgtttt ttaaatgtga tatttctttt tttagttgga ttttattccg
     1741 aaaaattata tgatgctttt ctcttcttta aagtgcgaaa aaggtttata ttttttcccg
     1801 ccgccctcaa agcctgcatt tttataaaat tctacaaaag taagacgcat gggatgaacg
     1861 aaagcaccca gaccacacat aaatatgttg attccatgtc caaaaatcat gataaccaac
     1921 attatgagtg tgctcactat aggtatgttg ccgcttaaat tgatggcaag atcgttgaaa
     1981 acaaaaccca taactgcacc agaaatacct agtgcaaata atctgatata agacagtaca
     2041 tctcccagta agccggtagc agtgttataa gtatcccata accctgatcc tatattgata
     2101 agtggatttc tttttatatt gttcagcaga aataccaaag ctccacctgc actgccaaaa
     2161 attatataca accatttggc agtttggggc gaaagccctg caaatgaaga aaggacaaaa
     2221 gtacctccgc aaccgatcaa caaaataagc catccccacg tagaaaagga agcaggccag
     2281 ccataacgtt taacttttcc aattgcttta attaccatcc caaaaagtat ttgaaccacg
     2341 ccaagcagta gcgatgtgta aaacaattga ttggaatcta acataaatgc tttaaaagat
     2401 gcgagccagg ggacatccat ttccagtaag ttaaatccaa aaaaagttcc actgacaaag
     2461 cccataataa cggtagaaat tcctaatagg gcagccaaac tcaatattgg ttttagtgct
     2521 gcatctgctt tttttctgaa ataaagagat gcaatcagta aaagaagtcc gtacgctgca
     2581 tcgccaaagc aaattccaaa aaaaagcatg taaaatggtg cgaagaaagg ggtcaaatct
     2641 aattcatgat aattaggtaa gtcatatagg ttgcctataa attcaaacaa gcgcgaaaag
     2701 cgattgtttt tcaattttac gggtactttt tcgtcctctt caggtttgct ggtctcgtag
     2761 taaatatccg tttcattaag aaattctttg agcttttctt ctgcatcttc cggacaccag
     2821 ccttccaaaa gcattacttt gttctccaca gttggctctg tgttaagcaa tactcttgca
     2881 aaatccatat tttcaataac ttttacttga gctgctttta atgagttgta attttcaaca
     2941 gaaagtgtta tgagttggcg attaatgtta tcaatttctt gatgtatttg ttgaatatct
     3001 tcatctattt gcaatacatt ttttgtgccg atttttacgg gatcagcatc tatttcaata
     3061 atttcgcctg tatgagttat ggttacaaaa taacgtgtgc tgccaatatt atcgatttca
     3121 aaagcattgt agcgtatttc ccattcctga tcgaattttc gcacattaca actgaagaaa
     3181 ttaatttcgt aacctgcctt ttgcagattt tctataagtt gataatcaaa aattccccac
     3241 atttccatgc ggtctcgttc tttttcagca agttggagtt tttgaattaa taattctttt
     3301 tccgcaaatt tattttcaat ttttttcaaa agttccattc catcaaccga tagatcggcc
     3361 ggtagaggag aagcatcttg aggtatcaat ttttccattt gtttgagagc tattttcatg
     3421 cgcgaagaaa gtagcatttt ttcacgtaat tcgtcgtttt cggcaatgcc ttccactttt
     3481 tcaattacgt gaacaacacc cacttccctc attttttcga ggaaatcgtg gtattgtttg
     3541 tgatacacca gaaaagtata tcgtgtcatt tgcaaaatca taaattttcc ttctttataa
     3601 gttcctgacg cgatttaact attttttgag acgatttgga taaattttcc tcatcttcca
     3661 tgaaacgttt gattttacga atagcatcct cgtagccggg aatttgtact ttttcaaaca
     3721 aattgacttt ttgggtggtt ttttttcgag caaactccag caggcttagc ttttgtatga
     3781 aaaattcctg ctctattccg atttgagcca gttttttaag aagggccaat ccatcggaat
     3841 accattttgg agaattaaat aggctgaaag gtttggtttt ataaactact tcttccaata
     3901 tcggtactcg tacagctgct atttttttta tgttcatctt tacttcttct acctccaata
     3961 aagtagtatc aaattcgccc catagagcaa gcattttatc gtattctgca atacgttgct
     4021 ccaattcatt ttcaattctt tttatctctt ccttagcttt tttcacttcg gtacgtaaag
     4081 cgctttcttt gtttttaatt gtgggtaaag cgcgcactct tatttttaaa tttttttcaa
     4141 gctgttgcag cgatgtttta ttatattgaa attgtatgga cataaaacgt ttatttaact
     4201 aaatgaaaaa ttttttaagc aatttaacga atgttgattt attcatgctg tttttgcttc
     4261 caatatttat ctatgaattc ctgtttaatg ttcacctctg ttgggctgaa atatttggca
     4321 aagagtttcc atgccgtgtc gagcatggcg gttgtgtcta tattgacatc gatagccaat
     4381 aatctgtcgg aatagtcttt ggcaaaagcc aatgctcttt cgtcatagtc ggtgagatca
     4441 aatccatttt caagttttgt tttggcattg gcagcatcag cataaagtcg aacagctgcg
     4501 ttcattacct gtgggtgatc ttcgcgagtt tttatgccga taaccagctg tttgaggcgc
     4561 gacaaggaac ggaaggggtc aacaattact ttgcctatat ccgaatcgcg acgcaggaac
     4621 aattgtcctt cggtaatata acctgtattg tcgggaatag catgggtaat gtcgcctccc
     4681 gagagagtag ttaccgcgat aatggtaata gaacctccgg cgggaaattg tacggctttt
     4741 tcatatattt ttgccaagtc ggaatacagt gagcccggca tggaatcttt agaaggaatc
     4801 tgatccattc tattggatac tatagccaat gcatcggcat ataatgtcat atcggtgagc
     4861 aaaaccaata ctttttcatt cttgtcgaca gcgaaatatt ctgcagcggt aagtgccata
     4921 tcaggaataa gtaaacgctc taccggaggg tcttctgtgg tattcacgaa actgacaatc
     4981 cggtctagta cgccggcatt gttgaaaacg tttttgaaat agagataatc atcatttgta
     5041 agtcccattc cccctaaaat aattttatcg gtttttgcac gcaaggcaac atttgccatt
     5101 acttcattgt atggttgatc gggatcggca aaaaatggta ttttctgacc ggataccagc
     5161 gtgttattca ggtcgatgcc cgcaattccc gttgcaatga gttccgaagg ttgtttccgt
     5221 cgtacgggat taactgatgg acctccaatt tctctttctt caccttcaat ttcggggcgg
     5281 tcatcaatag ggtctccaaa agcattgaaa aaacggcctg ccagttgatc gcttactttt
     5341 agggttggtg ctttcccgag aaagacaatt tctgcatcgg ttggtattcc ttcagttcct
     5401 tcaaaaactt gcaaggttac ttcatcgcct aagattttaa ctacttgagc caatttaccg
     5461 tttactaaag ccagttcgtc attgccaatg ccacttgcat gaagagaaca ggttgctttt
     5521 gtgattttgg taattttggt ataaattttt tgaaatgctt ttgttgtcat tttttctcag
     5581 attattattt tctctttttc cgggaaattt ctttcttttt agtctttata tttgtcaaaa
     5641 aaaattattg ttttatcttc ctttctgcaa gtatattttg cagttcattg tgataacttt
     5701 cgaaagcttg ggaatgaaat tcggaatagt tcatttgttt gcaaacatta ataattcgtt
     5761 tgaaataatc cataacatcg gtaaaattta caaagttgaa atctgatttg cagatatcca
     5821 taatcagttt taacatgtaa tgctgtcttt ctatcggagt tacggcatct atatcgtcaa
     5881 aggcatcttg ctgaagaata acaaagtcta tcaattcaga tttccagaaa gtaatgtgat
     5941 aatcgacggg aacaccatca tctcccagaa tattgatttg ttcctgaact tctttaccgc
     6001 gttgaagaag tgttcgcatg tcgtttacca tactgatcca ttggggagaa atacgctccg
     6061 agatgtattc gtcaaattcg ggatattcaa tatattttga ataactttca atgggattga
     6121 tggccggata tcttttacta tcggctcttg cctgttcaag tccaaaataa caccgggcta
     6181 cttttttggt gctttccgtt acaggttctt tcagattacc tccagccggc gatactgttc
     6241 caataaaggt gatggaaccg gtttgtttgt tttttaattt gacaaaaccc gatcggctgt
     6301 aaaaattggc tataatagcc gacaaatcca tcggaaaagc atcttgtccc ggtaattctt
     6361 ccatgcggtt actcatttcg cgtaaagcct gagcccagcg tgaagtagag tctgccatca
     6421 ataacactcg aagtcccatt gctctgtaat attcagctat tgtcatggcc gtgtaaactg
     6481 atgcttcacg ggcggcaaca ggcatatttg aagtgttggc cacaataatg gtacgttcca
     6541 ttaaaaatcg acctgtgtgt ggatctttca attcgggaaa ttctacaaac aattcaacta
     6601 cttcatttgc acgttcacca caagcagcca tgataacaat gtctgcttcg gcttgtttgg
     6661 aaatggcgtg ttgcaaaacg gtttttcctg ttccaaaagc accgggaata aatcctgttc
     6721 ccccttctac aattgggttg gctgtgtcta tgacgcgaac acctgtttcg agcaatttat
     6781 agggtctcgg tttttcggcg tgtacattta ttgccttttt tacaggccat ttttgtatca
     6841 tatttatttc aaaatccttt ccatgtgcat cggtaattat ggctatcgtt tcagatatcg
     6901 tatattcgcc tgccggggca atgcttttta ccttataact gccttccatg acaaagggta
     6961 ccataatttt gtgcggttga tggttttcgt cgacttcccc caaccatgaa gcagcttcaa
     7021 cttcatcccc tacctgagca ataggcttaa aattccattt tttatcttcg tccagtggaa
     7081 aatttcgttc tccacgttta aggaaaatac ctgttagttt gtttaagtca ttttgaagac
     7141 cgtcgaaatt tttcgacagc aagccgggcc ccagtattac ttctaacatg tgaccttcaa
     7201 atgttacttc attgtcaatt tttaatccgc gagtgctttc aaatacttgc acaaaaacat
     7261 cagaaccatt tactttcagc acttcagcca taagcttttc cgaacccagt tctatgaaac
     7321 aaatttcatt ttgtgatact gctccgtcaa cacttacggt taccaagtta gaaacgattc
     7381 cttttacttt tccttttgtt ggcatagttg tttgttgttt ccttattttt tctcttttga
     7441 actttcaaca aagagaggtc tttttaatta aacatatttt acttattaga attatctgat
     7501 tttttctccg gcctgaagag tttcttcaaa ttctacactt tgtttcattt tttctatcaa
     7561 ctccctgaaa attacatttc ccttttcggg agtcaatggt tgccagcgtt ctatcatttg
     7621 cattttcaga atgtaggcca atattttttc gatggtaaaa taataaaaaa atgtgtgttc
     7681 atccatccac tgccatttta aaagatcaat ttttttctct ctttccatta aatcgggttc
     7741 ttctgcaatt tgcaaaacct gttcaaggta ctcgaatgtg ccggtcaaac cataatcgcg
     7801 agcattgctt gttctcagtt gttgagctat tgtgttgctg cctacaatgt aattgtgggg
     7861 attcagtcca tattttctac aggctatggc agtaagcaaa ttattgatat tcaaactgaa
     7921 ttcaaaccat gattttaaaa aactgttgtt gcattgcaag gcaaaatcat agtataatcc
     7981 agtgaggtaa tcttctttag aaatgccttc cgcccagaat tcttcatcct gatagaccga
     8041 ataaaaatgc tggatgtaag gcaatattct tttgtctttg gggttttctg tttctttcag
     8101 caactgaacg atttctaccc aatcgttgta atttaaattt cctaaaggtt ggagaaaagc
     8161 atccctgtct atgaggaaag ccagaaagtt ttgattgtcg tattgggcaa aaatcaatct
     8221 cagaaattcc acatccgaag tatcaagcaa ttccaaaagt tcgtttttga aatcagcgag
     8281 agtaaatgta gttttcttat catcgggaga caagtcggga agtccggcaa taagacagta
     8341 ataattcata actttctttt ttgattcttg aatttacgta ttttttcttg atgttttttg
     8401 aaaaaaaata cggtatcatc atcttgttta aaaaagcatt tctattagtt tggggcgtaa
     8461 aaaatccttg aaatattctg tcaactcttc ttctccgaaa tttattttgt atcctccctt
     8521 gtccggtcga atagtaaagg ttgcttttga tctgtcggct tgggttatgg taacaccctg
     8581 attcagcaaa ttctttgcat tggctaagaa atattctgtt aaagctttgg catcgggagc
     8641 ttcaataact atagattctt ttttagacca ttctttggca agttgaagaa taacttgttg
     8701 catgaaattt ttatcgacca tagctgcctt cactgaatcg tcaactattt ttccgcaaat
     8761 cagattggta atttcggtct ttagtgcatt gagtgattgt tgtgcatata gcctgagttc
     8821 agatttggta ttttcttcga gctcagcagc tttcgcttcg gcttcggcta ttatgcgcgc
     8881 agctttttct tgagcggagg aaatgatcga atctgctttc ttttgagctt cttcaataat
     8941 agactgtgct tcagcattcc ctttttctac tccttccaaa taaatttttt cggtcaattc
     9001 ctgcaattta gtattcatat acaaagtatt tttctgatgt tttatattgc aaatttaatc
     9061 ttttctgtct aaatcaaaac gtttttagga aaatttaaaa aatgaaaacg catattccat
     9121 tttatattcc tgaattgttt aaaaaaatat ttattgaggc atgttgcagg gtcttttagt
     9181 tgaagaaaaa aattttacgg gcaattaact gcataatgtg ttacctcgaa aattttaaac
     9241 aaatttattc ttgagcgaag agtaactgca aactaaacaa tagtccgaat ttacaactat
     9301 ccggttttta taaaaatagg gttagatttt taaaaatatg atttgtaaaa ttaaatgatg
     9361 aataaacaat gtaaaagtaa agtcaaccga atttaattta tttaggttgc taataattct
     9421 tctttttccc aaagaactta tagattgttt tcttttcctt tactaaagaa ttttccttga
     9481 aaatataaat agaatttctt tcctctctct gcaatgattc gtcgaaatga ggaagagtgc
     9541 cggaaaaaat tcctttcagt ttcagggaat cttgcagaac gaaatcacaa ttcagtgaat
     9601 aattttcacc ctgacggctg accgtcatct ttccgccaac aataagtttt tgtgtggatt
     9661 tcaatggatt gggctcataa taattaatga cagctcctaa cgtatagacc tggttgtcaa
     9721 ttgtatcatt tattccggga gcaatggtga aaggttcgcc actgttatca actctgtaaa
     9781 ttccttctgg aagaagcgta tcggaaggag ctacaaaaac atcttcaaga aaaagatatt
     9841 gaccataacc cgaaagttca ttttggttgt ttatttttaa cgaatctgtg aacaaattga
     9901 ctgtcaaaac attgttctca ttcaaataat cggcatagta atctccgtaa aaaacgacat
     9961 agccccacgt ataagccgga ttcagctcgt attgataagg ttgattgggt gtgttgtcct
    10021 tacaggagat aaaagtcaaa atgcttacgg caattgccaa tttaatcgtt tttttcatgt
    10081 ttttttgatt agtatatatt gatgaaatct ataaaacagc tggctttata aacaaacttt
    10141 catagcactt ctcgataaat tataattcta cattcacagg cgaaggggca tcatgtatat
    10201 caatgtaacg aaccggatag taggctgcca gaaaacccat taacaaaaca gtcataaata
    10261 ccagcaaaac atcccaaaaa ttaaccaaaa caggataaac atccaccaca taaccggttc
    10321 cgagttttaa aatgccaaaa tgttgctgca gtaaacagag caacaatccc attataattc
    10381 ccagaacagc accaaccgcc gaaattagcc atccttcaaa aaggaaaatt cttcttatca
    10441 gttgctgctc agctcctaaa tttcgcaggg tttgaatgtc ctgttttttg tcaataatca
    10501 acatggataa cgaactgata atgttgaaac tggctatcag cagaataaaa cataaaatga
    10561 gataagtaat ccatttttcg attttcagga ttttaaaaaa atcttcctgc tgttcatatc
    10621 tgtctttaac ttcaaaatta cctcccagta tattttttat ttttttctgt gcatctaaag
    10681 ttttgatatt atcttttagc tttaattcaa ttgatgagac tttatttggt tgatattcga
    10741 acaaatcacg agccagctca atagaaacca gtacataatg atcatcatat tgcaattgct
    10801 taactgaaaa aattcctgat acaaatgttc cgatttggtt aaaactgttt tcaggacgga
    10861 gcaaattaat gtgtgaagtg cgttttgggg cgtaaatata caatgggtcg ataaaatttg
    10921 ctcccaatcc caaaatgctt gcaacgccga cgccggggac ggctctttgg aaagcgccat
    10981 cataaagtaa aaactttcca tcgaacataa tgctgtctat tttagtcatt ttttgaaaat
    11041 cgtcgcttac gcccttaatg atggcgggca tttgcttgtc tcgaaatcgc agaagagcat
    11101 tttcttcaat aacttcggta aaataggcaa cgtcgttcaa ctttttcact ttttcccatt
    11161 ccggagtatt tatatcgaaa gtttttcctt gcgaaacagt aattttcaaa tccgggtcga
    11221 aagccgaaaa catgccttgt atcaaggaat cgaaaccatt gaaaacggaa aggacacaaa
    11281 taagtgccat agttccaatg ccaaccccca aagctgcaat tccggatatt atattgatgg
    11341 cattgtgcga tttgcgtgaa aacaaatatc gccgtgccac ccataaggca aaacgaaatc
    11401 ctcctgatgt gttatctctt ttggtttggt ttggatgaat tgtttccatt gtttcttcgg
    11461 gcaaaatctg ctttcattat ccaattttca ataatcattg attgttttct tgcgaatggt
    11521 cctttttatc ggatggagaa tcatttttta aaagttgatc aatatgatcg atatagtcaa
    11581 gtgaatcgtc caaatagaaa aatagttcag gaataattcg cagttggtgt ttcactcttt
    11641 ttcccaagtc gaaacgaata tttttctggt cgttattaat tttttcaagg acttcagccg
    11701 attgtttgcc gggaaaaata ctcaaatgaa tgcgcgcaat acttagatcg ggacttatgc
    11761 ggacattggt tacgctaata atgataccgt gcatttcgcg agcatagagc agaaaaattt
    11821 ctcccaattc tttctgaaga agtcgcgcca ctttttgttg tcttgtatta tccataataa
    11881 attctccttt ttctcaggca aatactaacc ttcaattccg ttttttgcct ttttaatctg
    11941 tctttaattg tttaaccccc caaatcttat cctgtctctt caaattatat tctattttga
    12001 catcatttcc atcatcagct ttgcgtccag attaattttt gaaaaagcaa accannnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacatca tttccatcat cagctttgcg
    12181 tccagattaa tttttgaaaa agcaaaccat tttttaccca aatctttcag cgaagctcca
    12241 atatgataga tggtgttgtc gataatcaaa aaccgatcat gtgatttgga aaattccttc
    12301 acttcgacgg gtggatattg agcattaaat ttttccaaat acagtttgaa ttgcgctgaa
    12361 attttattgg taaaaatttg acatttgaca tcttcctttc ttttcgatag caaaatcaga
    12421 agctttcatc caaataattg tcaatcaaaa cgattgattt tgttgccgac ttcaccaaat
    12481 cagcaacaaa tttgtaggca tcaaaaatct gcccatcgta aaaaattccc tgcaccggag
    12541 gaagagctgt tttaacaaaa aaatcgattt tatcctctgt tgccgaaacg cgtctttcca
    12601 aattttcaat acgttggttg accgcataac ctttcaacaa ataatctttc aaaaccttgt
    12661 ttgcccatat cctgaattga gtaccacgtt tggattttat acgataacct acagatatga
    12721 ttacatcgag attatagtat tcaatttgtc ttattacatt ttttccacct tcaatttgaa
    12781 ctgttgcatt ttttgcaaca gttgaagaag catcaagctc ttgttcttta aaaatattac
    12841 ttatgtgtct tgaaataact gatttatctc tttcaaacaa tatactcatc tgattcagag
    12901 tgagccacac ggtttcattt tccaacatta cttcaagctg aacagcattg tcggactgat
    12961 aaagaattat ttcattgttg gcattcattc gctaagcagt caaattttga tacagctcca
    13021 tcaaattttc cattcaaatc catctttggt gtcctttatt tcaaagccca aagcagtcaa
    13081 ttcgttgcgg attttgtccg aagtagccca atccttattc ctttttgctt ccagtcgaat
    13141 ttgcagcaat aaatcaacgg ctttttttag tgcttcgtat ttggcaaaat tttcatcttc
    13201 ttttttaagt cccaacaagt cttccacaaa cactcgaaaa acgtctttca gttcttccaa
    13261 atcggcagac gaaacagtag cttttccatc ataaaccaga ttgataaatt tcactgcttc
    13321 aaacaaatgt gcaataacaa taggagtgtt caggtcatca cacatggctt cttcacagcg
    13381 ctgtcgaagt ccacttatat cggaggttga aatatcggat ggcatcaact tttgcaatct
    13441 atcatatcct tccattaagc gttccaatcc tttttcagag gcttgtaatg cttcattgct
    13501 gaaatcgact gtactgcgat aatgtgcctg cagaataaaa aaacgaaccg tcatgggctg
    13561 atacgcttga gaaagcaaag gatggtttcc ggagaaaaat tcttccaaag taatgaaatt
    13621 ccctaatgat tttcccattt tttgaccgtt gatggtaatc atgttgttgt gcatccaata
    13681 acgaacactt tccttaccga gtgccgcagt tgactgcgaa atttcgcatt cgtgatgggg
    13741 gaaaagcaaa tccatgcctc cgccatgaat atcaaattct tcacccaaat atttggtact
    13801 catcgccgag cattccagat gccagcccgg aaaaccttcg ctccagggcg atttccagcg
    13861 catgatatgt tcaggtgatg cttttttcca taaagcaaaa tccacgctgc gccgtttttc
    13921 ttcctgtcct tcaagcatac gcgttgtttc cagcagttcg tctatatttc tgcccgaaag
    13981 tttaccgtaa tgatatttct tatcgtattt ttcaacatcg aaatataccg atccttcact
    14041 ttcataggcg aatcccgcat cgagaatttt ttttacaaat tcaatttgtt cggggatatg
    14101 acccgaagca tgaggctcga tacttggagg caaaacattc aaagcttcca tagctttatg
    14161 gtaacggttg gcataatatt gagcaatttc cattggttcc aactgttcca gacgtgcttt
    14221 tttttcaatt ttatcttcgc cactgtcggc atcattttcc aaatgaccca cgtcggtaat
    14281 atttctcaca tatcgaacct tataacccaa atgtttcagg tagcgaaaca aaacatcaaa
    14341 tgttatggcc ggtcgtgcat gtcccaaatg tggatcgccg taaacggttg gtccgcaaac
    14401 gtacattccc acatgcggtg catgaagtgg aacaaacagt tccttttttc tgtgtaaagt
    14461 attgtaaatg tataagttat ttttcatttg gttataaaaa ttttttggat ttttattgat
    14521 aacttgcttt ttcaggtaaa ttttctttcc atttttaaag tttcgtaagc ctcgtttatg
    14581 gatcggaatt tttctgtggc ctttttcttc acatcttctc ccaatgtgtt gaccttgtcg
    14641 gggtgatatt tcatggccat tttccggtaa gctttcttaa tttcatcgac agaagcggaa
    14701 ggttcaattt caagagcttt ataggcccaa ttgatgtcaa cttgttttat atagggtgct
    14761 tttaaagatt caaaatccag tctggaaata cgaaatatgc cagctattct ttcaatcaat
    14821 gtcagttcgg aagtagaaac ctgatgatcg gcatacgcaa tgtcgaaaag caaatgaatc
    14881 agttccagcc gtgtagcata gtttgtataa cgataaatct gttctgcaac ctgacgttcg
    14941 tcaatatctt tttcaagcag attttttaat attgataatg cttccaatgc tccctcttct
    15001 ccaaaattgt taaccaaaaa acgtttgact atttccaatt ccgatttttt tggatgtccg
    15061 tctgctttca tcatacaagc aatcaaaacc aacagactta ttttgaaatc tcctgaagtt
    15121 gtggtagttc ttctattttg agatttctct actgtttcaa gtttagcgcc aatgaatgag
    15181 ccgatggcaa aaccaatcaa cgcaccgatt ggaccaccca aagcccagcc taagcctcct
    15241 ccaatccatt tggcaaaact gctcatatta cttccggtta agtattttgg aaaccacaaa
    15301 gatacgaaaa aagatagaaa tttacattcg tgtaaaattg agctgacaac ggcttaaaag
    15361 gagaatgatt ggttgttgaa tttatatatc tattcaattt aatttttttt aatttggttt
    15421 tcataaaaat caaaaataat cttgtaaatt tgcatgctac aagttattct ggttgtttaa
    15481 ctttatgctt ttaagaaatt caatcaacac taaaatattt tatcaacaaa tt
//
LOCUS       DAOSIB010000053        15511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_3967_length_15511_cov_12.106237, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000053 DAOSIB010000000
VERSION     DAOSIB010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15511
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3967_length_15511_cov_12.106237"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLD44_05940"
     CDS             <1..243
                     /locus_tag="PLD44_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98909.1"
                     /translation="LMLVLQKFSYISSTKKIYLFLLLKLSKMILWGIVAFVYLLVFRT
                     ERRMFGLLYGAYYLIYLIFETACFYMNEKRQVRQSK"
     gene            240..1331
                     /gene="atpB"
                     /locus_tag="PLD44_05945"
     CDS             240..1331
                     /gene="atpB"
                     /locus_tag="PLD44_05945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOF98910.1"
                     /translation="MKCCFVYIICFLAFSPFKILAQNNNYEGKRKEIDVREIILTEVS
                     DSYEWYITTWKSKSISIPLPVILHSNNSGWHVFMSSKFEEQPVYKGFYIAKEGKYKDK
                     IVELDENGKEIRPLDISLTKTATALIFNSVLLIILIMSIAHYYKKDGIFPKKGFVILM
                     EMLIVDIYEGVIKPSIGNDYRRYAPYLLTVFFFIFLNNLMGLIPFFPFGATVTGNIAI
                     TFTLALFTMLIVNFSGTKEYWKEIFWPEVPAWLKVPVPLMPAVEFIGIFTKPFALMIR
                     LFANIVGGHSIVGGLVCLVFVTVKLGTTINVTMSALSVIFTVFIDLIELLIAYIQAYI
                     FTMLSAVFIGLARVEPSHKQAAKEVVKQV"
     gene            1418..1666
                     /gene="atpE"
                     /locus_tag="PLD44_05950"
     CDS             1418..1666
                     /gene="atpE"
                     /locus_tag="PLD44_05950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOF98911.1"
                     /translation="MLLSILLQVAASDALAKFGAALGAGLAAIAAGIGIGKIGGSAME
                     SIARQPEMSSDIRMNMIIAAALIEGVALFAIVVCGFIL"
     gene            1777..2283
                     /gene="atpF"
                     /locus_tag="PLD44_05955"
     CDS             1777..2283
                     /gene="atpF"
                     /locus_tag="PLD44_05955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOF98912.1"
                     /translation="MSLLMPDFGLFFWMMLSFGIVVFILVKFGFPAILQMIEKRRSYI
                     EESLLEAENARKELEEIKTEAKKIQDEAFLNRKKILDETTEIKNALIKEAKEKAFIEA
                     EKIMEEAKKQIRYEKMMALQEIKQQVAEISIAVSEKLLQSKLNDKPAQMEIINRMLEK
                     MDIGKFGN"
     gene            2357..2908
                     /locus_tag="PLD44_05960"
     CDS             2357..2908
                     /locus_tag="PLD44_05960"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOF98913.1"
                     /translation="MQTGLIASRYAKALYEFASEMNAQEIVFANFQTLEKSFSANAEL
                     AQALKNPLLTRETKKQLIQFACGNKSDFILERFMDVLLQNRREYLLRNIGLKYSDYYY
                     ENKNLALVKLTTASPMDAETEKKLISQIKTYIKKELKVEKIIAPEIIGGFIIEIGNRR
                     WDGSLATQLKKIQKELNENILLT"
     gene            2950..4542
                     /gene="atpA"
                     /locus_tag="PLD44_05965"
     CDS             2950..4542
                     /gene="atpA"
                     /locus_tag="PLD44_05965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOF98914.1"
                     /translation="MSDNKTIKVSEISELIKKQLQQIDIESYYEETGKVLEVGDGVVR
                     IYGLTNAEANELLEFENGVKAIAMNLEEDNVGAVLLGPTHLIKEGFTVKRTGRVASIK
                     VSEKMLGRVINPLGEPIDGKGKIDGEFFEMPLERKAPGVIFRQPVEEPLQTGLKAIDS
                     MIPIGRGQRELIIGDRQTGKTSIAVDTIINQLDNFKAGDPVYCIYVATGQKGSTVATL
                     FNTLKDFGAIPYTILVVATAADSAALRYLSPYAGAAIGEYFRDTGRHALVVYDDLSKQ
                     AIAYRELSLILRRPPGREAYPGDIFYLHSRLLERAAKIINQQEVAKQMNDLPECLKGK
                     VKPGGSLTALPIIETQAGDISAYIPTNVISITDGQIFLDTDLFNEGNRPSIDVGISVS
                     RVGGNAQIKPMKKVAGTLKTDQAQFRELEAFTKFGSEMDAVTAMTIDKGQKNTQLLIQ
                     SLHRPLSVEKQVALLYCGTHGLLANIPLHKVKEFEENFINLLDYSYKEKILNEIKKGN
                     FDEKVTSSIEEAAEILKKQMNY"
     gene            4590..5450
                     /gene="atpG"
                     /locus_tag="PLD44_05970"
     CDS             4590..5450
                     /gene="atpG"
                     /locus_tag="PLD44_05970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOF98915.1"
                     /translation="MSSLKELKSRIQSVKSTQKITSAMKMVSSVKLLKAEKAVYNFLT
                     YRETLTDILRNYLSSQIVDRSPFREIRHVNHVGIVVFSSNSSLCGNYNSNIIKRLEQS
                     ITTHRDVRKEDILIFPIGKKVEDFCKEKNLAIAGEFQNVAAHPDIENTTQIADTLIQL
                     FLQHKIDRIKVIYFHYVNKTTQIMIAETFLPIEINYRKKGEFRNDYLIEPSIEEIEQQ
                     LIPLALHYHIYTAALEAYASECAARTLSMQAATENAENLLQQLSLEYNKMRQESITNE
                     ILDLIGGSLQ"
     gene            5595..5667
                     /locus_tag="PLD44_05975"
     tRNA            5595..5667
                     /locus_tag="PLD44_05975"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5628..5630,aa:Lys,seq:ttt)
     gene            5870..7096
                     /gene="pepT"
                     /locus_tag="PLD44_05980"
     CDS             5870..7096
                     /gene="pepT"
                     /locus_tag="PLD44_05980"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOF98916.1"
                     /translation="MTLTDRFLRYVSFTTTSDELTNMTPSTPGQMVFAQHLVKELKSF
                     GLKDAEVDKNGYVMATLPANTDKKIPTIGFIAHLDTSPDMSGKNVKPQIIENYDGKDI
                     LLNEEQLIVLETEKFPEILQYVGQDIIVTDGTTLLGADDKAGIAEIISAVEYLLRHPE
                     IKHGKIRIAFTPDEEIGKGADNFDVKKFNADWAYTIDGGEIGELEFENFNAASAKITF
                     HGLNVHPGYAFHKMKNSIRIAHQFVAMLPRHETPEHTEGYEGFYHLTSIEGTVEKTTM
                     HLIIRDHDRDKFEKRKKELQHLANKINAEFGENTAVIELRDQYYNMREKIEPVFHIID
                     LASDAMKEVGITPKIVPVRGGTDGARLSYMGLPCPNIFAGGMNFHGRFEYIPIQSMEK
                     AMMVIVKIVELAAKKY"
     gene            7715..8146
                     /gene="dut"
                     /locus_tag="PLD44_05985"
     CDS             7715..8146
                     /gene="dut"
                     /locus_tag="PLD44_05985"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOF98917.1"
                     /translation="MKIKIVNLSKHPLPSYATLLSAGMDLRANIDEPVVLKPLERKLI
                     PTGLFIELPEGYEAQIRPRSGLAIKHGVTVLNSPGTIDADYRGEICIILINLSNDDFV
                     INDGERICQMVIASHEHAEWEEVKMLNDTDRGNGGFGHTGR"
     gene            8217..9983
                     /locus_tag="PLD44_05990"
     CDS             8217..9983
                     /locus_tag="PLD44_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOF98918.1"
                     /translation="MHKKIFLFPILIFLCLLTISCSSTRKSVTREKNFRSNKLTPEQQ
                     SQFDYYFYEALRLKNNQQLDEAMETFLFCRQIDSLDAEVQSQLGVLYANIGLIDPALK
                     ALKKSVELQPTNWWYSMTLLNIYSQQKNWKDAIALANEMQKIYPEKEEIYSTLAQLYK
                     ASGDIDKAIDAYNRLENLTGIDESISFAKFSLYAQQNKVKKAIAEIDRLIEKYPTENR
                     YKVLRGDIYLQLNNLEQAFAIYEQILKEEPQNPFVYISLSEYYKKINQPEKATEAIVS
                     ALKNEQLDVDTKIEILGQYIDNLIKDKEKLDETEALFKLLVDRYPLEEQVHAYYALFL
                     EYQERIPEAISELETMLNINPKNLQTWLKLIQIYWSDKNFEKIYVTTERAIEVLPEEP
                     ILYFYQGIALYQLNDYQASLQKNKKALLYIKDQQKALKSDVYAQLGDTYFKIGQKDSA
                     FIAYEESLKANPENIYVMNNYAYYLSLEKKDLKKAERMSAKTVEKEPNNATFLDTYAW
                     ILYQQGNYSLAKFYIERAIDNLKDGEDNGIIYEHYGDILWMYGGNDEKALEMWIKSYE
                     LGNQTEELKQKIENKGFDRQNK"
     gene            10028..10825
                     /locus_tag="PLD44_05995"
     CDS             10028..10825
                     /locus_tag="PLD44_05995"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HOF98919.1"
                     /translation="MKKILSIFLMILVLLSSCKTKKQVQQPSYFPQKLTTEKVIEQLN
                     ALDNIQTAEIKKMNIYLKMKDREFNVTSSMTIKKDSAINISIQPFFGMELFKLEITPN
                     NIFIFDKVNQTYYTTDFQYLNLRTGLGIDFYHLQALLTNRYFCLARQKDIACSILAQN
                     NHKISFGFDNETLMQTMSINPDFNITEILLVDKINNLSFNALYENFALINGIIFPRQI
                     TYMLKGQKHLSSAKITIDRVKFNQQVEFIPTDTRRYKKGSFDQLFKK"
     gene            10885..12114
                     /locus_tag="PLD44_06000"
     CDS             10885..12114
                     /locus_tag="PLD44_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOF98920.1"
                     /translation="MFRTYLIQLLLIIAFACSGTQLLAQNVKQLEEERKQTLQKLETT
                     SKMLDETKKSQKSSLNKLSLLNKNINERTHLIKTMSKEISQLDVEIKTMGQEIEQLER
                     RLENLKNDYARLVQEAYINRSIYSKIMFVLSAETFDQSLRRIRYLKEYSDYRKHQVSE
                     IEKVKAQIEQKKDSISKHKSTLVVVVKEKEVEATKLSQEQKKEKKLLTDLQKKEKNLR
                     KELDAQQKKANDLNKRIESLIAEEIRKAENLNKSKGKSTTSKVSTLTKEEVLLSGNFE
                     KNAGRLPWPVSNGFISGHYGIQPHPVLKHVTTNNKGIYIQTPANSTARAVFDGVVTQL
                     FSVPGSNNAVIIQHGEYRTVYANLTDIYVKIGDKVSTKQAVGKIFTDSEKDNKTELYF
                     QIWKNKNLLDPEKWLAR"
     gene            12183..14066
                     /locus_tag="PLD44_06005"
     CDS             12183..14066
                     /locus_tag="PLD44_06005"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOF98921.1"
                     /translation="MEEIVEKVTLTEYNDSNIITLEGLEHIRRRPGMYIGKLGDGSHP
                     DDGIYVLLKEVMDNCIDEFRMGAGKTIEVQIVNDMVQVRDYGRGIPLGKMIEAVSIMN
                     TGGKYDSKAFKKSVGLNGVGTKAVNALSEKFIAQSFREGQTRKAAFEKGKLTFDSGIV
                     ESDAERGTLITFTPDHEMFDGYEYRDEVVETMLRNYVYLNAGLTIVYNGKKIISRNGL
                     TDLLNENMTVPALYPIIQLKGEDIEVAFTHCDQYGEEYYSFVNGQHTTQGGTHQSAFR
                     EAVAKTIKEFYSKNMEYTDIRNGIVAAIAVNIEEPVFESQTKTKLGSRDIAPGGISVN
                     KFISDFIKTELDNYLHRNLETAEILLQKIQDSEKERKAIAGVTKLARERSKKVSLHNK
                     KLRDCRVHYNESKPGAEESSIFITEGDSASGSITKSRDVNTQAVFSLRGKPLNCFGLT
                     KKVVYENEEFNLLQAALNIEDGIEGLRYNKIIVATDADVDGMHIRLLLITFFLQFFPE
                     LIKKGHVYILQTPLFRVRNKKEKIYCYSEEEKIAAVKRLGATAEITRFKGLGEISPDE
                     FKNFIGKDIRLEQVSLRREDAVSELLEFYMGKNTSERQNFIIENLVIEEDKVEEEIEL
                     KIE"
     gene            complement(14155..15237)
                     /locus_tag="PLD44_06010"
     CDS             complement(14155..15237)
                     /locus_tag="PLD44_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98922.1"
                     /translation="MKKILYLLLLTFICASCNDMKDKPEPRNFPENTNEKGKMYVLSE
                     GLYHLNNSTLACIDFDERKMDFNFFAVKNGRGLGDTANDMKRYGDRLWIVVCGSSQVE
                     IVHISTGKSIKRLALTNQNDEPRYPRAITFWENKAYVCSFDGSITEIDTASFVMKTNI
                     YCGRNPDGIVAVNGKLYVSNSGGLDFPNYDSTVSVIDLAIYREIKRIKVGQNPYKLQS
                     DSEGDVYVVSRGNNGTIPAVWQRIDSKTDELLQTFEDLPVGNFVIQNDTAYMYNFDFI
                     TNNFWFKSFDCKTERIINERLITDETEIERPYSISVHPSNGNIYICDAKNYTKNGTLF
                     CFDKNGRLRYKIPQIGLNPNNLIIVE"
BASE COUNT         5331 a         2819 c         2919 g         4442 t
ORIGIN      
        1 ttaatgcttg tgctacaaaa attttcttat atttcatcta ctaaaaaaat ttatctgttt
       61 ttactgttaa agctttcaaa aatgatttta tggggaatcg tagcttttgt ttatttgttg
      121 gtttttagaa cagaacgaag gatgtttggt ctcctttatg gtgcttatta tctaatttat
      181 ctgatttttg agactgcttg tttttatatg aacgaaaaac gtcaagttcg gcaaagtaaa
      241 tgaaatgctg ttttgtctat attatttgtt ttcttgcttt ttcccctttt aaaatacttg
      301 cccagaacaa taattatgag gggaaaagaa aagaaattga tgttcgggaa atcattctta
      361 cagaagtgag tgattcctat gaatggtata ttactacctg gaaaagcaaa tcaatttcga
      421 ttcctttgcc tgtcattctt cacagcaata attcgggatg gcatgttttt atgtcttcca
      481 aatttgaaga acaacctgtt tacaagggtt tctatattgc aaaagaagga aaatacaagg
      541 ataaaattgt ggaactcgat gaaaatggta aagaaataag gcctttggat atttccttaa
      601 caaaaacggc tactgcgctt atttttaatt ccgttttgct gattatttta atcatgagta
      661 tagctcatta ttacaaaaaa gatggaattt tccccaaaaa aggatttgtt atcctgatgg
      721 aaatgctgat tgttgacatt tacgaaggtg ttatcaagcc aagtatcggg aatgattacc
      781 gacggtatgc gccctatttg ctgacggttt ttttctttat ttttctcaat aacttgatgg
      841 gattgattcc gtttttcccc tttggagcca ctgtaacagg aaacattgcc attacattta
      901 cgctggctct ttttacaatg ttaattgtaa acttttcagg aacaaaggaa tattggaaag
      961 aaattttttg gcctgaagta cctgcttggc tgaaagttcc tgttcctttg atgccagcag
     1021 tagagtttat tggaattttt accaaaccct ttgctttaat gattcgtctc tttgccaata
     1081 ttgtgggagg gcattctata gttggcggat tggtttgtct ggtgttcgtt acggtaaaat
     1141 tgggaacaac cataaatgta accatgtcgg ctctttcagt gatttttacg gttttcattg
     1201 acctgataga attgctgata gcatacattc aggcttatat ttttaccatg ctttcggctg
     1261 tgtttatcgg attggcaaga gtagagcctt cccataaaca ggcagcaaag gaagtagtta
     1321 aacaagtttg aacaatttga aaatgcgaaa aactaatatt agactttaat taattcaata
     1381 tcattataaa aatttcattt aaaaacaaga agttatgatg ttactatcta ttttattaca
     1441 agttgccgca agtgatgcat tggcaaaatt tggagccgca ttgggggcag gtcttgcagc
     1501 tatagcagcg ggtattggta tcgggaaaat cggcggatcg gctatggaaa gtattgcgcg
     1561 tcagcctgaa atgagttcgg atattcgaat gaacatgatt atcgctgcgg ctcttattga
     1621 aggagttgct ttgtttgcca ttgtagtttg tggatttatt ctttaatcaa aaaaattttt
     1681 caaggcttgt ctcaggaggg ttattcattg ataatattgg aaaaatcaaa agtatttgat
     1741 tctattaatt gtgtgcttaa tcattaaaaa aatgctatgt ctcttctaat gcccgatttt
     1801 ggtttatttt tctggatgat gctttcattc ggaatagttg tttttattct tgttaaattt
     1861 ggtttccctg ccattcttca aatgatagaa aaacgaaggt cttacatcga agaatctttg
     1921 ttggaagccg aaaatgcacg caaagaactc gaagaaatta aaacagaagc aaaaaaaata
     1981 caggatgaag cttttttaaa ccggaaaaaa atacttgatg aaactactga aattaaaaat
     2041 gctttaataa aagaagcaaa ggaaaaagcc tttatagaag ctgaaaaaat catggaagaa
     2101 gcaaaaaaac aaatcaggta tgaaaaaatg atggctttac aggagataaa acaacaggtt
     2161 gctgagattt caattgcggt atcggaaaag ctgttacaat cgaaattaaa tgataagccc
     2221 gcccaaatgg aaattatcaa tcggatgttg gaaaagatgg atatcggtaa atttggaaat
     2281 tgaaaaattt atttattccg aacagcaata aaactccact ttaaaaattt tattatctat
     2341 tgtaacaatt atatgcatgc aaaccggttt gatagcttca cgttatgcaa aagccctgta
     2401 tgaattcgct tctgaaatga atgcgcagga aattgttttt gccaactttc agacacttga
     2461 aaaatctttt tctgccaatg cagaactagc gcaggcatta aaaaatcctt tgttgacaag
     2521 ggaaacaaaa aagcaactta tccaatttgc ctgtggtaac aaatctgatt tcattttaga
     2581 gcgttttatg gatgtattgt tgcaaaaccg tcgtgaatat cttttgcgaa atataggttt
     2641 aaaatattcc gattattatt acgaaaacaa aaacttggca ttggtaaaac tgactacagc
     2701 ttctcctatg gatgccgaaa cagaaaaaaa attgatttct caaatcaaaa cttatattaa
     2761 gaaagaatta aaggtggaaa aaataattgc tcctgaaatt attggaggtt ttatcatcga
     2821 aattggaaac cgaagatggg acggaagctt ggcaacgcaa ctaaaaaaaa ttcaaaaaga
     2881 attgaacgaa aatattcttt taacctgaaa aaattctttt cgtaaaccta aaaatataat
     2941 tataagaata tgtctgataa taagactatt aaggtaagtg aaatatcgga gctgataaaa
     3001 aaacaactcc aacaaatcga catcgagagt tattatgaag aaaccggaaa agtacttgaa
     3061 gtaggggatg gagttgtcag aatatatgga ttgacaaatg cagaagccaa tgaattgctt
     3121 gaatttgaaa atggagtaaa agccattgcc atgaatttgg aagaagataa tgttggagct
     3181 gtgttgcttg gtcccaccca cttgataaaa gaaggtttca ctgtaaaaag aaccggcaga
     3241 gttgcctcca tcaaagttag cgaaaaaatg ttggggcgtg ttatcaatcc tttgggtgaa
     3301 cctattgatg gcaaaggtaa aatagatgga gaattcttcg aaatgccttt ggaacgaaaa
     3361 gcaccgggag taatttttcg acagcctgtg gaagaaccat tacaaacagg tttgaaggcc
     3421 attgattcaa tgattcctat tggacgtgga caacgtgaat taataattgg cgaccgtcaa
     3481 acagggaaaa caagcattgc ggtcgatacc atcatcaatc aattggacaa ttttaaagca
     3541 ggagatcccg tttactgcat ttacgtagcc acagggcaga aaggttctac tgtagcaacc
     3601 ctcttcaaca ccctaaaaga ttttggagca ataccttaca ccattctggt ggtggctaca
     3661 gcagccgatt ctgctgcttt gcggtatctg tcaccttatg ccggagctgc catcggcgaa
     3721 tattttcgcg atacaggaag acatgcactg gttgtttacg atgacttgtc caaacaagct
     3781 atcgcttatc gagaactctc gttgattctt cgacgtcctc ccggtagaga agcttatcct
     3841 ggcgatatct tttatctgca ttcccgatta ctggaaaggg ctgcaaaaat cattaatcag
     3901 caggaagttg ccaaacaaat gaatgacttg cctgaatgcc ttaaagggaa agtgaaaccg
     3961 ggtggttcgc tcacggcatt gcccattata gaaacgcaag ccggcgatat ttcggcgtat
     4021 attcccacaa acgttatttc tattacggat ggccagattt ttctcgatac cgatttgttc
     4081 aacgaaggga atagaccgtc tattgatgta ggaatttccg tttctcgtgt aggagggaat
     4141 gcacaaatta aacccatgaa gaaagtagcc ggcacattaa aaaccgatca ggcacaattt
     4201 cgcgagctgg aagcttttac taaatttgga agtgaaatgg atgcggtgac ggctatgacc
     4261 atagataagg ggcaaaaaaa tacccaattg cttattcagt ccctacatcg gccgttatct
     4321 gtggaaaaac aggtagcgct actctattgt ggaacacatg gattgctggc aaatatacca
     4381 ctccataagg ttaaagaatt tgaagaaaat ttcattaatc ttttggatta ttcttacaag
     4441 gaaaaaattc tgaatgaaat aaaaaaagga aattttgacg aaaaagttac ttcttccatt
     4501 gaagaggcag cagaaatatt aaaaaaacaa atgaattatt aaagccatct cccaacaagc
     4561 tttattttaa aaaatcaatt tcacacatca tgtcttcatt aaaggaactt aaatcaagaa
     4621 tacaatcggt aaaatctact caaaaaatta cttcagccat gaagatggtc tcttcagtaa
     4681 aacttttaaa ggcagaaaaa gccgtttaca atttcttgac ttatcgggaa accttaacgg
     4741 atatattgag aaattattta agtagccaaa ttgttgacag atcgcctttc agagaaatca
     4801 ggcatgtgaa tcatgttggc attgtggtat tttcttccaa ttcttctctc tgcggtaact
     4861 ataattcaaa cataatcaaa agactggaac aatctattac cacccatcgt gacgtaagaa
     4921 aggaagatat cctaattttt ccgatcggca aaaaagtgga ggatttttgc aaagaaaaaa
     4981 atttggccat tgctggggaa tttcaaaatg tggctgctca tcccgatatc gaaaacacca
     5041 ctcagatagc cgatactctt atccagcttt ttctccaaca caaaatcgat cgaataaagg
     5101 ttatatattt tcattatgtg aacaaaacca cacaaatcat gatagctgaa acctttttgc
     5161 caatcgaaat aaactatcga aaaaaaggcg aatttcgtaa cgattatctc attgaacctt
     5221 ctatcgaaga aattgaacaa caattaattc cattggcttt acattatcat atttacacgg
     5281 cagctctcga agcttatgct tcggaatgtg cagcccgtac cctgtccatg caagcagcta
     5341 ccgaaaatgc cgaaaacctt ttacaacaac tttcgcttga atacaacaaa atgagacagg
     5401 aaagcattac aaatgaaatc cttgacctga taggcgggtc attgcaataa gttttttccc
     5461 gttttctact tcactgctaa gttcaactta gaagtgtgaa tttagcagaa agttttgcat
     5521 tgattttttg aaaattcgaa aagaaattgt acctttgcaa gcgttttaaa aaaaaaacag
     5581 acatttttat tattgatcta gtagctcagc aggtagagca catccctttt aaggatgggg
     5641 tcctgggttc gaatcccagc tggatcactt cttttttcaa cagatttatc atctttcccg
     5701 cttgcttcct acaaaaaagt tgcattaatt tcttgaattt aaatccaact aaatactctc
     5761 tttgaaacaa ctatcggaat acgttttttc gcacataaag aaaaaattat ccggaaatag
     5821 tttcttaata aaaaatctgt aactttgaag caataaaaaa tcgactttca tgacattaac
     5881 cgacagattt ttaaggtacg taagttttac aacaacttcc gacgaattaa ccaacatgac
     5941 acccagtacg ccgggtcaaa tggtttttgc tcaacatttg gtaaaagaat tgaagtcttt
     6001 tgggttgaaa gacgccgaag tggataaaaa cggctatgta atggcaactt tgccggccaa
     6061 taccgataag aaaattccaa ctatcggctt tattgcccat ttggatacca gccccgatat
     6121 gagtggaaaa aacgtcaaac ctcagattat tgaaaattac gatggcaaag atattttact
     6181 gaacgaagaa cagctaattg tgctggaaac agaaaaattt cctgaaattt tgcagtatgt
     6241 gggtcaagat attattgtta ccgatggcac tacattgctt ggtgccgatg acaaagcagg
     6301 cattgctgaa attatttctg ctgttgaata tcttctgcgt catccggaaa ttaaacatgg
     6361 aaaaattcgc attgctttca ctcctgatga agaaatagga aaaggagcag ataatttcga
     6421 tgtgaagaaa ttcaatgccg attgggcata tactattgat ggtggagaaa ttggagaatt
     6481 ggaatttgag aatttcaatg ctgcctcagc caaaataact tttcacggat taaatgttca
     6541 tccgggctat gcatttcata aaatgaaaaa ttccattcgt attgctcatc agtttgtagc
     6601 catgcttccc cgccacgaaa cgccggaaca taccgaaggt tacgaaggtt tttatcattt
     6661 aacgtccata gaaggtacgg ttgaaaaaac gaccatgcat cttatcattc gcgaccacga
     6721 ccgcgacaaa tttgaaaaaa ggaaaaaaga gcttcagcat ttggctaata aaatcaatgc
     6781 agaatttgga gaaaataccg ctgttatcga attacgagat caatactaca acatgcgtga
     6841 aaaaattgaa ccggtttttc acattatcga tttggcatcc gatgccatga aggaagtggg
     6901 cattacgcct aaaattgttc cggtaagagg gggaacagat ggcgcccgac tttcgtatat
     6961 gggacttccc tgtccgaata tttttgccgg agggatgaat tttcatgggc gtttcgaata
     7021 cattcccatt caatctatgg aaaaagctat gatggtgatt gtaaaaattg ttgagttggc
     7081 tgcaaaaaaa tactaaaatt tctctgacaa aaaatagtgg cctgttcagt aataatgaat
     7141 gattttgcaa atgaatttga taatcgattg attacatctc ggacttcata caaggactct
     7201 actctttcca ttttcctttt tgacaatttt tggatagcaa gcatacagat gcttgtttaa
     7261 aagcgcattc acgagtattt ggaacatttt ttgaagtgta acaaaaagta aaaagaaaat
     7321 gtaagccttt acggatttct gaataattaa ccattttttc agcaggagga aacaacgaaa
     7381 gaaaaatcta taaaaggaac attacaaaaa acttggaaaa tactttagaa tagagaataa
     7441 ggaaccaaaa ttaaaacaat agaaatatta actgcattaa agatattaaa gcgaaaaagg
     7501 gaaataaaac agggttacaa atcaaacaaa taccaatcaa ctatctttga ggtattaacg
     7561 ctttatccgg attcataaaa ttccggtatt gttttattcg atttaaaaac atttactgat
     7621 gaaatccaca tgtattgcct acacaaacac gaatgaatat aatatgtcag acatgtgggt
     7681 tccatcagac ctttaaaaaa taaattatat tctgatgaaa ataaaaattg tcaacctttc
     7741 gaaacatcca ctgccatcct acgccacttt actttctgcc ggaatggatt tgcgagccaa
     7801 tatcgacgag ccggtagttt taaaaccttt ggaaaggaaa ctgattccca caggactttt
     7861 tatcgagttg ccggaaggat atgaagcaca aatacgcccg cgaagcggac tggccatcaa
     7921 acacggcgtt acggtgctca attctccggg cactattgat gccgactatc gcggcgaaat
     7981 ttgtattatt ctgatcaatc tttccaatga cgattttgta atcaacgatg gcgaacgaat
     8041 atgccaaatg gtgattgctt cgcatgaaca tgcagaatgg gaagaagtaa aaatgttgaa
     8101 cgataccgat cgaggaaatg gcggatttgg acatacggga cgataaattc caaaaaatgt
     8161 agaaattctg aatcgaatct actattatcg gaaaataaca agcaaaacaa tttttcatgc
     8221 acaaaaaaat ttttttattc cccatattaa tttttctctg tcttctcaca atttcctgct
     8281 catcgacaag gaaatcggtt acacgagaga aaaattttcg gtcaaataaa ctgacacccg
     8341 aacagcaaag tcagttcgac tattattttt acgaagcgct acgactcaaa aataatcagc
     8401 agctcgatga agcgatggaa acttttctct tttgccgaca aatcgattcg ctggatgcag
     8461 aagtacaatc gcaactgggt gtactatatg ccaatatcgg gctgattgac cctgccctga
     8521 aagcattgaa aaaatcggtt gaattgcaac ctaccaactg gtggtacagc atgacgctgc
     8581 tcaacattta ctctcagcag aaaaattgga aagatgctat tgctttggcc aatgagatgc
     8641 aaaaaattta tcctgaaaaa gaggaaatct attccacgct tgctcagttg tacaaagcct
     8701 ccggcgacat cgacaaagcc attgatgctt acaaccgact cgaaaaccta accggcatag
     8761 atgaaagcat ttcctttgca aaatttagcc tttatgcgca acaaaacaaa gttaaaaaag
     8821 caatagccga aatcgacagg ttgatcgaaa aatatccgac cgagaatcgc tacaaggttt
     8881 tgcgaggcga tatttacctt caactaaaca atctcgaaca ggcttttgct atttatgaac
     8941 aaatattaaa ggaagaacca caaaatcctt ttgtctatat ttccctttcg gaatattaca
     9001 agaaaatcaa tcagcccgaa aaagctacgg aggctattgt cagcgcatta aaaaacgaac
     9061 agttggatgt ggataccaaa atagaaattc tggggcagta cattgacaac ctgataaaag
     9121 acaaagaaaa actcgacgaa acggaagcac ttttcaaact cttggtcgat cgttatccgc
     9181 tcgaagagca ggtgcatgcc tactacgctt tgtttctgga ataccaggag cgtatcccgg
     9241 aagccatatc tgagcttgaa accatgttaa acatcaaccc taaaaacctg caaacatggt
     9301 taaaacttat tcaaatatat tggagcgaca aaaattttga aaaaatatat gttactactg
     9361 aacgcgccat cgaagtattg cccgaggaac ccatattgta cttttatcaa ggaattgctt
     9421 tgtatcaatt aaatgattat caagcttcac tgcaaaaaaa taaaaaagct ctgctttata
     9481 taaaagacca acagaaagcg ctgaagagcg atgtatatgc acaactggga gatacttatt
     9541 ttaaaatcgg tcaaaaagat tcggcattca ttgcttacga agaatcgctg aaagccaatc
     9601 ccgagaatat ctacgtgatg aataattatg cgtactatct gtcgctggaa aagaaagatt
     9661 tgaaaaaagc cgaaagaatg agtgccaaaa cagtggaaaa agaacccaac aatgccactt
     9721 ttctcgatac ttatgcatgg attttgtacc agcagggtaa ttattcgctg gcaaaatttt
     9781 acatcgaacg ggctatcgac aacttgaaag atggagagga caacggcata atttatgaac
     9841 attatggcga catactctgg atgtatggag gaaatgacga aaaagcactg gaaatgtgga
     9901 taaaatcata cgaactcgga aatcaaacgg aggaattgaa acaaaaaatt gaaaacaaag
     9961 gtttcgatcg tcaaaacaaa taacaaaatt tgcagaaaaa ttttttgctt ccaaaagtta
    10021 caagtttatg aaaaaaattt taagtatttt cctgatgata ttggtattgc tcagttcctg
    10081 caaaacaaaa aagcaggttc agcaaccctc ttattttcca caaaagctaa ctaccgaaaa
    10141 ggttatcgag cagttgaatg cattggacaa tatacaaact gcagaaatca aaaaaatgaa
    10201 catttacctg aaaatgaaag atcgggaatt taatgtaact tcttcaatga caataaaaaa
    10261 agactctgct atcaatattt ccattcagcc tttttttggc atggagcttt tcaaactgga
    10321 aataacacca aataatattt ttatattcga caaagttaac caaacctatt atacaacaga
    10381 ttttcagtat ctgaatcttc ggactgggct tggtattgat ttttaccatc tgcaagccct
    10441 gctgaccaat cgctattttt gtttagctcg acagaaagat attgcatgca gcattcttgc
    10501 acaaaataac cacaagataa gctttggatt cgataacgaa acactgatgc aaaccatgag
    10561 cattaacccc gatttcaata taacagaaat tttactggtc gataaaatta ataacctatc
    10621 tttcaatgca ttatacgaaa attttgcttt gataaatggg ataatttttc caagacaaat
    10681 tacatatatg ttgaaaggac aaaaacattt atccagcgca aaaattacca ttgatagagt
    10741 gaaatttaat cagcaagttg aatttatacc aaccgatacc cgtcgttata aaaaaggaag
    10801 ttttgatcag ttgttcaaaa aataaacaat tcagcctcat aatttgctat tcatttttta
    10861 tataattttt tttattttgt caagatgttt cgtacttatc taatccagct tttattgata
    10921 attgcttttg cctgttcagg gacgcaactc ttagcccaga acgtaaaaca attggaagaa
    10981 gaacgcaagc aaacgcttca aaaattggaa acgaccagca aaatgctgga cgaaaccaaa
    11041 aaatcgcaga aaagttcatt gaacaagctt tctctcctca ataaaaacat caacgaacgt
    11101 actcatctga ttaaaacaat gagtaaggaa atttcgcagc ttgatgtaga aataaaaaca
    11161 atgggacaag agatcgaaca attggaacgt cgattggaaa atttaaaaaa tgattatgca
    11221 aggctggtac aggaagcata tatcaaccgc agtatttatt ccaaaattat gtttgtgctt
    11281 tcggcagaaa cttttgatca atccctacgg cgcattcgat atttaaagga atacagcgac
    11341 tatcggaagc atcaagttag cgaaatagaa aaggttaaag cacagattga acaaaaaaaa
    11401 gacagtatat ccaaacacaa aagcacactg gtggtagtgg tgaaagaaaa agaagtagaa
    11461 gcaacaaagc tgtcgcagga acaaaaaaaa gaaaaaaaac tgttgacaga tcttcagaaa
    11521 aaagagaaaa atttacgtaa ggaattggat gcacagcaaa aaaaagccaa cgatttaaac
    11581 aaacgaatag aatccctgat agccgaagaa attcgaaaag ccgaaaatct taataaaagt
    11641 aaaggaaaat cgacaacaag caaagtttct acactaacaa aggaagaagt tttattgtcg
    11701 ggcaatttcg agaaaaatgc gggacggctt ccctggcctg tcagcaacgg ttttatcagc
    11761 ggacattatg gcattcagcc tcaccccgta ttgaaacatg ttacaaccaa caacaaaggt
    11821 atttacattc aaacacccgc caatagcacg gcaagagctg ttttcgatgg cgtagttact
    11881 caacttttct ccgtacctgg aagcaacaat gccgtcatta tacaacatgg tgaatatcgt
    11941 accgtttatg caaacttgac cgacatttat gtcaaaatag gggataaggt ttctactaaa
    12001 caagcagtag gtaaaatttt taccgacagc gaaaaggaca acaaaacgga gctttatttt
    12061 caaatatgga aaaataaaaa tttgttggat ccggaaaaat ggctcgcacg ataaaccaaa
    12121 aatcaattaa atttgcacat tggtttaaag tgaatttttt ttataatcta ttctatggaa
    12181 atatggaaga aatagttgaa aaagttacgt tgacagaata taacgacagc aatatcatta
    12241 cgctggaagg gctggaacat atccgtcgcc gtcccggtat gtacatagga aaactcggag
    12301 acggttctca ccccgacgat ggaatttacg tcctactaaa ggaagtgatg gacaattgca
    12361 ttgatgaatt ccgtatggga gcgggaaaaa caatcgaagt tcagatcgta aacgatatgg
    12421 tacaggttcg cgattacggt cgaggtattc cgttgggaaa aatgatagaa gcggtttcga
    12481 ttatgaacac tggcggaaaa tacgactcca aagcctttaa aaaatcagtt ggactaaatg
    12541 gcgtaggaac gaaagcagta aatgcgctct cggaaaaatt tattgcgcaa agcttcagag
    12601 aaggacaaac cagaaaagcc gcatttgaaa aaggcaaact tactttcgac agcggtatcg
    12661 ttgaatcgga tgccgaacgc ggaactttga ttaccttcac acccgaccac gaaatgttcg
    12721 acggatacga ataccgcgac gaagtggtgg aaaccatgct tcgaaattac gtttacctaa
    12781 atgcgggact taccattgtt tacaacggga aaaaaattat ttcccgtaac ggtttgaccg
    12841 atttactgaa cgaaaacatg accgttccgg ctctataccc tatcattcaa ctgaaaggag
    12901 aagatattga ggttgctttt acccattgcg accaatatgg agaagagtat tattcttttg
    12961 ttaacggaca acatactacg caaggcggaa cccatcaaag tgcttttcgc gaagcggtag
    13021 caaaaaccat caaagaattt tattccaaaa acatggaata taccgacatt cgcaacggaa
    13081 tagtggctgc cattgccgtc aacatagaag aacctgtttt tgaatctcaa accaaaacca
    13141 aactcggttc gcgcgacatt gcaccgggag gaatatctgt caataaattt atttccgatt
    13201 tcattaaaac cgaattggac aattatctcc atcgcaacct tgaaacagct gaaatactgc
    13261 tccaaaaaat tcaggattcg gaaaaagaac gaaaagccat agccggcgtt acgaaattag
    13321 cacgtgaacg ttccaaaaaa gtcagtttgc acaacaaaaa attacgcgac tgtcgcgttc
    13381 attacaacga atcgaaaccc ggcgccgaag aatcgtccat cttcattacc gaaggtgatt
    13441 ctgcaagcgg ctccattaca aaaagtcgtg atgtgaatac gcaggcagtt ttcagtcttc
    13501 gcggcaagcc actgaactgt ttcgggttaa ccaaaaaagt ggtgtacgaa aacgaggaat
    13561 tcaacctgtt gcaagctgct ctcaacatag aagacggcat cgaaggactt cgttacaaca
    13621 aaatcattgt tgccaccgat gccgatgtag atggtatgca tattcgcttg ctgcttatta
    13681 cttttttcct tcagtttttc cccgaactga ttaaaaaagg gcacgtttac attctacaaa
    13741 caccgctttt cagggttcgg aacaaaaaag aaaaaattta ctgttattcg gaagaagaaa
    13801 aaattgcagc cgttaaacga ttgggagcca ctgccgaaat tacgcgcttt aaaggcttgg
    13861 gtgaaatttc gcccgacgaa tttaaaaatt ttataggcaa ggacatccgc ttagagcagg
    13921 tttcgttacg cagagaagat gccgtttcag aattgctcga attttatatg ggaaaaaata
    13981 ccagcgaacg ccaaaatttt attatcgaaa atctggtcat tgaagaagat aaagttgaag
    14041 aagaaattga gttaaaaata gaataactcc ttttttattt atgtctcaga aaatatcgga
    14101 gaacaaggca aattgtttat tcggcgagaa atgaaaaaat acattttttg agcattactc
    14161 aacaattatc aggttattcg gattcaaccc aatttgcggg attttgtatc ttaatctccc
    14221 gtttttgtcg aagcaaaata aagtaccatt cttagtatag ttctttgcat cgcaaatata
    14281 aatattccca tttgaaggat gaacagatat ggaataagga cgttcaattt cagtttcatc
    14341 tgtaataagc cgttcgttaa tgatgcgttc ggttttgcag tcgaaactct taaaccagaa
    14401 gttgttggtt ataaagtcga aattgtacat ataagctgta tcgttctgga ttacaaaatt
    14461 ccccacaggc aagtcctcga aagtctgaag tagctcatcc gttttgctgt cgatgcgttg
    14521 ccatacggcc gggattgttc cgttatttcc tcgagaaacc acgtaaacgt ctccttcgct
    14581 gtccgattga agtttatatg ggttctgacc tacttttatt ctttttattt cacgatatat
    14641 cgccaaatca attaccgaaa cagtgctgtc gtaatttgga aaatcgagac cgccggaatt
    14701 ggatacgtat agtttcccgt ttactgcaac aattccatca ggatttcgac cacagtaaat
    14761 attcgttttc atgacaaaac tggcagtgtc gatttccgtt attgaaccat caaaagaaca
    14821 cacataagct ttattctccc agaaagtaat ggcacgggga taacgtggtt cgtcattttg
    14881 gttggttaat gccagtcttt ttatcgattt tcccgttgaa atatgtacaa tttccacttg
    14941 agaagagcca caaaccacta tccacaaacg gtctccatac cttttcatat cgtttgcagt
    15001 gtcgccaaga cctctgccat ttttaactgc aaaaaaatta aaatccattt ttctttcatc
    15061 aaaatcgatg caggcaaggg tgctgttgtt cagatgatac aatccttcgg ataaaacata
    15121 cattttcccc ttttcattcg tattttcagg aaaatttcga ggttcgggct tgtccttcat
    15181 atcgttgcat gaggcacaaa taaaagtcag taagagtaaa tagagtattt ttttcatctt
    15241 tttccagaac tttgagtctg caaaattaca tttttccagc taaaaagtac cattagtcca
    15301 caaatagatc gattatacaa gcactaatca cttgttgtcc tgtgctgtcg cacaatttta
    15361 tttgataaac tcttctttgt tttctcgata acccacttga tttgccaatt cgacttccag
    15421 aagcattata caaatcatct cagattgctt tacatatttg acgtttctct ccgtcgtaca
    15481 tgcaatcacc atttaacttt tcaatttacc t
//
LOCUS       DAOSIB010000054        15492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_3969_length_15492_cov_11.100408, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000054 DAOSIB010000000
VERSION     DAOSIB010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15492
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3969_length_15492_cov_11.100408"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            318..2474
                     /gene="rnr"
                     /locus_tag="PLD44_06015"
     CDS             318..2474
                     /gene="rnr"
                     /locus_tag="PLD44_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOF98923.1"
                     /translation="MSKKTNRNTSTRIPRKQLINAISDIFNQHPRETYNYRQIAKMLN
                     IENQSQQQYIRQLLEGLADEEFVTEISKGRFKANFPNGNSIIGTIDRQGVKTYVIPDD
                     GGEMIFIPERKTNHALLNDKVKVVLYARRKGQQQEGEVVEVLQRAQDTFVGTLQVSEN
                     FAFLVVDNRIMTSDIFIPRNKLNGGKNGQKAVVKLMEWQPDLKSPVGEVIDVLGDKGN
                     NNTEMHAILAEFGLPYRYPVEEEKAAEKIDDGITPEEIAKRRDMRKVTTFTIDPRNAK
                     DFDDALSIRKLNNGLWEIGVHIADVTHYVQPRTIIDREAEKRATSIYLVDRTIPMLPE
                     HLSNGICSLRPNEDKLTFSVVFQIDENADVKGYEIVHTVINSNRRFTYEEVQEIIESG
                     KGDYSAEILQLNKMAQILRKRRFENGAIAFERTEVQFEIDKKGRPISVYFKEQKESHQ
                     LIEEFMLLANRTVATHIGKPAKGRKAKTFVYRVHEVPSPDKLRDLSAFVKKFGYHFKT
                     SGKQTSVSKGINKLLDEVQGKKEQNLIETLAIRSMSKAVYSTNNVGHYGLAFDYYTHF
                     TSPIRRYPDMMVHRLLDRYLANGKSVNAEEWEEYCLHSSDMEQLAAKAERTSIKYKQV
                     EFMSERIGQIYDGIISGITEWGIYVEIIENKCEGMIPIRDLDDDYYIFDEKNYCLTGK
                     RYHKRYRLGDEIKIRVAKANLDKRELDFALAGNFGK"
     gene            2712..3113
                     /locus_tag="PLD44_06020"
     CDS             2712..3113
                     /locus_tag="PLD44_06020"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HOF98924.1"
                     /translation="MKNLNLFFLAVIFLALGACSSNSNSPEAVAEKFNKAIYTADFNA
                     AKALCTDDSKQAVDLIASFVSEKAGDMKKANVKYETKSVNLSEDGNSADVEGVVLNAY
                     DLQNDKIADSTDTKVHLVKENGKWLVDYKLK"
     gene            3086..3703
                     /locus_tag="PLD44_06025"
     CDS             3086..3703
                     /locus_tag="PLD44_06025"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HOF98925.1"
                     /translation="MAGRLQAQIICFLLLSCFQFLPESCHSPTKIENSDTYITNYLRS
                     GDIILRKSYGLVSNIIAAKLNDTIDVSHCGIIYIDANHTVYVIHSLSKAVSEKDGMQS
                     CTLKKFMADSRIETVQVVRFKKDSAQIIGKWAHYYLLKQIPFDNNYDLKDSSSFFCTE
                     LPIHIINKQFGINLSSGNEFPKFSIFLNTKYFDKVFFIKKANLNH"
     gene            complement(3737..4816)
                     /gene="leuB"
                     /locus_tag="PLD44_06030"
     CDS             complement(3737..4816)
                     /gene="leuB"
                     /locus_tag="PLD44_06030"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOF98926.1"
                     /translation="MGKNINIAVLPGDGIGPEIIDQALKVTEAVCNKFQHDLTYEFGL
                     VGACAIDEVGEPYPKATHELCMQSDAVLFGAIGNPKYDNDPSAKVRPEQGLLKMRKEL
                     GLYANIRPVNTFPSLVEKSPLRAELVDGADFICIRELTGGIYFGRPQGRSEDGSTAYD
                     TCIYTREEIERIVHLAYRFAAQRRKKVTMVDKANILATSRLWRQITQEISAQYPVVET
                     EYMFVDNAAMRIIQSPKNFDVVVTENLFGDILTDEASVITGSLGMLPSASIGVHTSLF
                     EPIHGSYPQAAGKNIANPMAAILSAALMFEYAFVLKEEGALIREAVSACIEAGVVTED
                     IANGQKAYKTTEVGQWIVNYIDNKK"
     gene            complement(4926..6467)
                     /locus_tag="PLD44_06035"
     CDS             complement(4926..6467)
                     /locus_tag="PLD44_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOF98927.1"
                     /translation="MVEIMDTTLRDGEQTSGVSFSAQEKLSIARMLLQEMCVDRIEIA
                     SARVSEGEFDTVKKICQWARNNGYIDKIEVLGFVDGKDSLNWIQSAGGKVVNLLSKGS
                     LKHCTYQLKKTPEQHVTDIKQIIAHATEMGISVNLYLEDWSNGMKDSEDYVYFLMDAL
                     KSENIKRFMLPDTLGVLNPEDTKNFCKKMIERYPNLHFDFHAHNDYDLAVANVFEAVK
                     VGVKGVHTTVNGLGERAGNAPLTSVLAVLHDHLKIKTNLREDKAYALSKMVETYSGIR
                     IPHNKPLIGENVFTQVAGVHADGDNKRNLYFNDLLPERFGRERQYALGKTSGKANILK
                     NLQQLGIDLDDNILRKVTERVIELGDKKQLTTMEDLPYIVSDVLNEQEEQSIKLLNYS
                     LSITYGLRPVATVKMEINGEVYEQTAAGDGQYNAVSKAMWKIYKILNKPTPELLDYEV
                     VIPPGGQTNAIVQTIITWKFNQVIFKTRGLDRDQTIAAIKATMKMLNLIEGGKLRSNK
                     YTDLP"
     gene            complement(6531..7121)
                     /gene="leuD"
                     /locus_tag="PLD44_06040"
     CDS             complement(6531..7121)
                     /gene="leuD"
                     /locus_tag="PLD44_06040"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOF98928.1"
                     /translation="MEKFVKLTSTVVPLPIENVDTDQIIPARFLKATNKDGFGDHLFA
                     DWRYNKDGSPKADFVLNNPTYKGSILVAGKNFGSGSSREHAAWAIAGYGFKVVVSSFF
                     ADIFKNNALNNGVLPVVVTPEFLNEIFQSVSENPNMTLTVDLENQTITNNATGKSEKF
                     DINVYKKECLIKGLDDIDYLLSKKDLIEAYEASRTF"
     gene            complement(7212..8612)
                     /gene="leuC"
                     /locus_tag="PLD44_06045"
     CDS             complement(7212..8612)
                     /gene="leuC"
                     /locus_tag="PLD44_06045"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOF98929.1"
                     /translation="MNTLFDKIWDAHVVSTIENGPTILYIDRHYVHEVTSPQAFNGLR
                     ARGLKVFRPLQTTLTADHNIPTLHQDQPVKDPVSRNQLDTFEKNARDFQLPLFFPMGH
                     EKNGVVHIIGPENGFTQPGMTIVCGDSHTSTHGAFGAIAFGIGTSEVEMVLATQCILQ
                     TRPKTMRITFDGKLNKGVTAKDLALYMISKLSTGGATGYFVEYAGEAIRNMNMEERMT
                     VCNLSIEMGARGGMIAPDETTFAYLKNREFAPKGEEWNKKLAYWKTLYSDQDAVFDKE
                     YTFDASEISPMITYGTNPGMGMGIDQTIPTLEQVDEAGRISFEKALNYMGFRPGEKLL
                     GKPIDYVFLGSCTNGRIEDFRTFAQFVKGKKKADNVVAWLVPGSRKVEKQIHDEGLHE
                     ILMEAGFELRQPGCSACLAMNDDKVPAGKYAVSTSNRNFEGRQGPGARTILAGPLVAA
                     AAAVTGKITDPREFLS"
     gene            complement(8772..10265)
                     /locus_tag="PLD44_06050"
     CDS             complement(8772..10265)
                     /locus_tag="PLD44_06050"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOF98930.1"
                     /translation="MDRLFIFDTTLRDGEQVPGCQLNTVEKIEVAKALENLGVDIIEA
                     GFPVSSPGDFNSVVEISKTVTWPVICALTRAVEKDIEVAAEALQYAKKKRIHTGIGTS
                     EYHIRYKFNSNQEEILERSVAAVKYAKKFVEDVEFYCEDAGRTENEYLARVVEAVIKA
                     GATVVNIPDTTGYCLPWEYGEKIKYLMEHVDGIHKAIISTHCHNDLGMATANTISGIM
                     NGARQAEVTINGIGERAGNTALEEIAMILKCHKKLNIETQINTKKIYSTSRLVSNLMN
                     MPVQPNKAVVGRNAFAHSSGIHQDGVLKNRENYEIMNPEDVGIDHNSIVLTARSGRAA
                     LKHRLNTLGVELADKKLDAVYEEFLKLADKKKDINDDDLLMLAGKERSEKERIKLDYL
                     QVTSGVGVKPVASIGLIIGSEKFEAASAGNGPVDAAIKALKKIINREMTLQEFTIQSI
                     NKGSDDVGKVHMQVEHEGVVYYGFGANTDIVAASVEAYIDAINKFVE"
     gene            complement(10603..11199)
                     /locus_tag="PLD44_06055"
     CDS             complement(10603..11199)
                     /locus_tag="PLD44_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOF98931.1"
                     /translation="MYRFIESIKLLDGIFYRLKFHQKRMNICSELFYPNDNFIHLQSA
                     LTDSHPPQKGLFKCKITFESKILKIEYLPYKKKEINSLRIVHTEIESRPYKKEDRTDF
                     NAAFDMRGNYDDVLLMKNGLLTDTSICNIALFDGFSWQTPTKPLIYGVNRANLLEEGK
                     IFEKDIKLSDLQNYSAIRLFNALIEFGEIELNISNIHF"
     gene            complement(11183..>12058)
                     /locus_tag="PLD44_06060"
     CDS             complement(11183..>12058)
                     /locus_tag="PLD44_06060"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOF98932.1"
                     /translation="KNQDLDDQYIRFQLSDESFSNQEKSTKNSFNFQWNIHPVNFDEY
                     KLKFHFVMENIRCGNSFLLNLTQPTEVSTNLNLLELYELSVSKYKLWLKDLFTVFSPE
                     TFIKIEDGIISTFPMKGTIDASVANAEDAILNDFKEQAEHATIVDLMRNDLSMVAEKV
                     EVKKYRYIDKIITNQNELFQTSSEITGELPENYCCYLGNIIISMLPAGSISGAPKSKT
                     LEIIREIEGYDRGFYTGIFGQYDGKNLDSAVLIRFIEQKNGQMIFKSGGGITSNSEVL
                     KEYREMLEKVYVPIY"
     assembly_gap    12059..12158
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12244..12672
                     /locus_tag="PLD44_06065"
     CDS             12244..12672
                     /locus_tag="PLD44_06065"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOF98933.1"
                     /translation="MKIRQLSVFVENKTGHLNQILAVLARNNINIIALTLADSIDFGL
                     LRMLVSDAEKAAEALRTENFTVRIHEVLSLELEGVPGSLSRVLNHFSDNGIAIEYIYA
                     FSFGSKSILVMRPDNTEKAIEIIQKYQLKPIYESDLKAQL"
     gene            12669..13967
                     /locus_tag="PLD44_06070"
     CDS             12669..13967
                     /locus_tag="PLD44_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOF98934.1"
                     /translation="MIWNEEIECMSSSEMRKLQSERLKELVNKVYQNVEFYRKKMDEK
                     GVRPSDIRSIDDISKLPFTNKTDLRDNYPFGLFAAPMKEIVRIHASSGTTGKPTTVGY
                     TKKDIENWQEVLARCLIMSGVTPEDIVQVSFGYGLFTGGLGIHYGAENVGCTVVPISA
                     GNTRRQLQLMSDFGSTVLACTPSYALHLADAMSEFGYSLSDMKLKTGVFGAEPFSENM
                     RLELEKKWGIKAFDIYGLSEIMGPGVANDCEFHDGLHIHEDHFFPEVVDPKTKEPLPD
                     GQQGELVFTTLTKEGIPLLRYNTHDLTTLNRERCECGRTLVRMKKITGRSDDMLIIRG
                     VNLFPSQIEQVLLEMRETSAHYMLYVDRENNLDILELKVELDETKMVDTIKDLMSLSR
                     KIEHSLHSALGLSVKVTLVEPKSIPRSEGKAVRVIDNRKI"
     gene            13987..15297
                     /locus_tag="PLD44_06075"
     CDS             13987..15297
                     /locus_tag="PLD44_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOF98935.1"
                     /translation="MSISKDLVDEIFRQFGQEDINKLSIREMGKLVAQIEQKTNVEFI
                     HMEMGVPGLPPLQTTVDAEKEALDRGLAALYPDIEGIPFVKNETARFAKLFMDIDVTP
                     LNCIPTVGAMQGSFAALLAAARTNKNKPCTLFIDPGFPVNKSQLQLIGLPFKSFDILE
                     NRGERLRSALTSFLKEGDICTITYSSPNNPSWINFTEEELQIIGELATEYDVIVVEDL
                     AYFGMDFRKDYGRPGVPPYQPTVARYTENYILLLSASKIFSYAGQRIGMMIISESLRT
                     RHYPELKNYFTSDVLGHFIPYGALYATTAGTSHTAQYAFAVTLKAVNDGKINFLEALK
                     KYAEKAKIMKEIFLKNGFYIVYKEKNEELADGFYFTVAYPEMTSSQLMKNFLYYGLSA
                     ISLSITGSKDNHGLRICVSFVRPDQIPVLDKRLKEFAEDMKKNV"
BASE COUNT         4715 a         3008 c         2808 g         4861 t
ORIGIN      
        1 ttattacgaa atttgtataa ccaaaaagtt gcatttgtga cttgaattca gcaacgaaaa
       61 gttataaaca atgtaataaa attgaaatat tcaaaaacca aaatttaata ctttctgctc
      121 aattcggacg ctaagttgaa cgtaaccatc aaaatttttt gaagaaaaag tcgtaacttt
      181 gcatttttat ttctcctgac cgcaaaaata ctatccggag actttttaat acagcaaaaa
      241 tttattgata atcaaacatt aattcaaaaa aaataatgga aaatttttaa aatcttccat
      301 tcaaaaaaca aataaacatg tcgaaaaaaa caaatagaaa taccagcacg cgaattccgc
      361 gtaaacaatt gataaacgcc atcagcgata ttttcaacca gcatccgcgc gaaacataca
      421 attaccgaca aatagccaaa atgctgaata ttgaaaatca atcgcagcaa caatacatcc
      481 gacaactgct cgagggattg gcagatgaag aatttgttac ggaaatttcg aaaggacgtt
      541 tcaaagccaa tttcccgaat ggaaacagca taatcggaac cattgaccga caaggcgtta
      601 aaacttatgt gattcccgac gatggcggag aaatgatttt tatacccgaa cgcaaaacca
      661 accatgccct gcttaacgat aaggtaaaag ttgttctgta tgctcggcga aaaggccaac
      721 agcaggaagg agaagtggtg gaagttttgc aacgcgcaca ggatacgttt gtcggtactt
      781 tacaggtttc tgaaaatttt gcctttctgg tggtggacaa ccgcatcatg accagtgata
      841 tttttatccc cagaaacaaa ctcaacggtg gaaaaaacgg tcaaaaagct gtggtaaaac
      901 tgatggaatg gcaacccgat ctgaaaagtc ccgtaggcga agtaatagat gtcttgggag
      961 ataaaggcaa taataatacc gaaatgcacg ccattctggc cgaatttggg ttgccgtaca
     1021 gatatccggt tgaagaagaa aaagcagccg aaaaaattga cgacggaatt acacccgaag
     1081 aaatagcaaa aagacgcgac atgagaaagg taaccacttt taccatcgat ccgcgcaatg
     1141 ccaaagactt tgatgatgca ctttccatca gaaaactcaa taatggactt tgggaaattg
     1201 gcgttcacat cgccgatgta actcattacg ttcagcccag aaccattatt gaccgtgaag
     1261 cagaaaaaag agccacttct atctatttgg tggacagaac gatacctatg ttgcctgaac
     1321 acttatctaa cggaatttgt tcgcttcgac ccaatgaaga caaactgact ttttccgttg
     1381 tttttcaaat tgatgaaaat gcagatgtaa aaggctacga aattgttcat accgtaatca
     1441 atagtaatcg tcgatttacc tacgaggaag ttcaggaaat catcgaaagt ggaaaaggtg
     1501 attattcagc agaaattctt cagttgaaca aaatggcaca aattctccgt aaaagacgtt
     1561 tcgagaacgg agccattgct tttgaacgta ccgaagttca atttgaaatc gacaaaaaag
     1621 ggcgacccat ttcggtttat tttaaagagc aaaaggaatc ccatcaattg atcgaagaat
     1681 ttatgctgct ggccaaccgt actgtagcca cacacattgg caagccggca aaaggtagaa
     1741 aagctaaaac ctttgtctat cgtgtccatg aggttccaag tcctgacaaa ttacgcgatt
     1801 tgtctgcttt tgttaaaaaa tttggctatc atttcaaaac ttccggtaaa caaacctcag
     1861 tttcaaaggg aatcaacaag ctgttggatg aagttcaggg gaaaaaagaa caaaatctga
     1921 tagaaacgct ggctatccgc tccatgtcca aagcggttta ttcaaccaac aatgtcggtc
     1981 attatggcct tgctttcgat tattataccc atttcacttc tcccattcgc cgttatcccg
     2041 acatgatggt gcatcgtctg ctcgacagat atttggccaa cgggaaaagt gtcaatgcag
     2101 aagaatggga agaatattgc ctccacagct cagacatgga gcaattggca gccaaagccg
     2161 aacgcacttc catcaaatac aaacaggtgg aatttatgtc tgaaagaatc ggacaaatct
     2221 acgatgggat catatccggc ataaccgaat ggggaattta cgtagaaata attgaaaaca
     2281 aatgtgaagg aatgattcct attcgcgatt tggacgacga ttattacatt tttgacgaaa
     2341 aaaactactg tcttaccgga aaacgctacc ataaacgcta ccgcttggga gacgaaatta
     2401 aaattcgagt tgccaaagcc aatctcgaca aaagagagct cgattttgca ttggctggta
     2461 actttggaaa atgacacaaa agaaaaatca ttcaggagaa aaagattttt aatcaagttt
     2521 atccgacaaa ctttaaatta tgtcgatatt tatacttgta attcctattt ttagaagttc
     2581 tcatttttta taattatttt gaattaattt tagtttttgt ttgtttttcg taaatttatt
     2641 ttatactttt acgcctttaa acagccgaca attaaaattc ggtaattaat tcaactaaaa
     2701 tttaaaagat tatgaaaaat ttgaatttat tttttttggc agtcattttt ctggcacttg
     2761 gagcttgttc ttccaatagt aattctcctg aagcagtagc agaaaaattt aacaaggcta
     2821 tttatacggc cgatttcaat gcagccaaag ccttatgtac cgacgacagc aagcaggcag
     2881 tagatttaat tgcttctttt gtttccgaaa aagcaggtga tatgaaaaaa gcaaatgtta
     2941 aatacgaaac aaaatcggtc aatttatccg aagatggaaa ttcggcagat gttgagggtg
     3001 ttgtgttaaa cgcttacgat ttgcagaacg ataaaatagc cgattcaacg gatacaaaag
     3061 ttcatttggt taaagaaaac ggcaagtggc tggtagatta caagctcaaa taatttgctt
     3121 tcttttgttg tcgtgttttc aatttttgcc ggagagttgc cattctccga caaaaattga
     3181 aaattcagat acttatatta ccaattatct tcgtagcggc gacataattt tacgaaaaag
     3241 ttacgggctt gtcagcaaca taatagcggc taaattgaac gatacgattg acgtttcgca
     3301 ttgtggcatt atttatattg atgccaatca taccgtgtac gttattcatt ccctttcaaa
     3361 agcagtttcg gaaaaagatg gcatgcaaag ttgcacgctt aaaaaattta tggccgacag
     3421 tcgtattgaa acggtacagg ttgtcaggtt caaaaaagat tccgctcaaa ttattggcaa
     3481 atgggctcac tattatcttt taaaacaaat cccttttgac aataattatg atttaaaaga
     3541 ttcttcttca tttttctgta cagaacttcc cattcacatc ataaacaaac aattcgggat
     3601 caatctttct tcgggcaatg agtttccaaa attttctata tttctgaata ccaagtattt
     3661 cgataaagtc ttttttataa aaaaagcgaa ccttaatcat taaagtccgc ctgaagcaaa
     3721 gattataaat cttattttat tttttattat cgatgtaatt aactatccat tgtccaacct
     3781 cagtagtttt gtatgctttt tgaccgttgg caatatcttc ggttaccacg ccggcttcga
     3841 tacatgccga aacggcttcg cgaatcaaag caccttcctc tttcaacaca aaagcatatt
     3901 caaacattaa agccgccgaa aggatggctg ccattggatt ggctatattt ttacccgctg
     3961 cttgcggata ggaaccatga attggctcaa aaagtgaagt atgaacacca atggaagcag
     4021 atggcaacat tcccaacgaa ccggtaatca ctgaagcttc gtcggtaaga atatcgccaa
     4081 agagattttc cgttaccact acatcgaaat tttttggcga ctgtataatc cgcattgccg
     4141 cattgtcgac aaacatgtat tctgtttcca caaccggata ttgagccgaa atttcctgag
     4201 ttatttgtct ccaaagtcga gaagtagcca aaatattggc tttatcaacc atagttactt
     4261 ttttgcgacg ttgtgcagca aagcgataag ccaaatgaac aattctttca atttcttccc
     4321 gtgtgtaaat acaggtatca taagccgtac ttccatcttc gcttcttcct tgaggacggc
     4381 caaaatagat tcctcccgtt aattcgcgga tgcaaataaa atcggctccg tccaccaatt
     4441 ctgctcgcaa tggagatttt tcaaccagtg aagggaaagt atttaccggc cgaatgttgg
     4501 catacaatcc cagttctttt ctcattttaa gcaaaccctg ctcgggacgc actttagcag
     4561 aaggatcatt atcgtatttc ggatttccta tggctccaaa aagtacagca tcggattgca
     4621 tacacaactc atgtgttgct ttggggtatg gttctccaac ttcatcaatg gcacaagctc
     4681 caactagtcc aaattcgtaa gtgaggtcat gttgaaattt gttacaaaca gcttctgtta
     4741 ctttcaatgc ctgatcgata atttccggac caataccatc acccggcaaa acggcgatat
     4801 taatattttt tcccatttta tttattgttt ttaaagtgat tttctttttt tcttactctt
     4861 tacaaaaatg agcataattt ctgaactttc gcacgatgca gtataatatt tttcaaaata
     4921 aagaattaag gtaaatctgt atatttattt gaacgtaatt taccgccttc gatcagattc
     4981 agcattttca tggtagcctt gatggctgca atggtctggt ctcgatccaa tcctctggtt
     5041 ttgaaaataa cttgattgaa tttccatgta ataattgttt gaacgatagc gtttgtttga
     5101 ccaccaggtg gaattaccac ttcataatca agcaattcgg gtgttggttt attcagaatt
     5161 ttataaattt tccacatggc tttggaaaca gcattgtatt gtccatcgcc ggcagccgtc
     5221 tgttcatata cttctccgtt tatttccatt tttaccgtcg caacagggcg taatccataa
     5281 gttatggata gcgaataatt gagcagttta atggattgtt cttcctgttc gttgagtaca
     5341 tcagatacaa tgtaaggaag atcttccata gtggtgagct gttttttgtc ccccaactca
     5401 ataacacgtt ctgtaacttt tcgcaaaatg ttgtcatcaa ggtcaatgcc caattgctga
     5461 agatttttca aaatatttgc cttgccggat gttttcccca aagcatactg tctttcacga
     5521 ccaaatcttt ccggaagcaa atcgttaaag tagagatttc tcttgttgtc tccatcggca
     5581 tgaacgccgg caacctgcgt gaatacattt tcgccgataa gtggcttgtt gtgcggaata
     5641 cgaataccgg aataggtttc aaccatttta ctcaacgcat aagctttgtc ttcacgtaaa
     5701 ttggtcttta ttttcaaatg atcgtgaagt acagccaaaa cacttgtcaa tggagcattt
     5761 ccggctcttt ctcccaaacc gttgacagtg gtatgaacgc cttttacacc gacttttact
     5821 gcttcaaaaa catttgcaac agccaaatcg taatcattgt gtgcatgaaa gtcgaaatgt
     5881 aaattgggat aacgctcaat catttttttg caaaagtttt ttgtatcctc aggattaagc
     5941 actcccaacg tatcaggtaa catgaaacgt ttaatgtttt cggatttcag agcatccatc
     6001 agaaaatata cataatcttc cgaatctttc atcccattcg accagtcttc caaataaaga
     6061 ttgacagaaa ttcccatttc cgttgcatgg gcaattatct gtttaatatc ggtaacatgt
     6121 tgctcgggcg tttttttcaa ctgataagtg caatgcttta acgaaccttt tgaaaggaga
     6181 ttgacaactt ttccgccggc cgattgaatc cagttgagtg aatcttttcc atcaacaaac
     6241 cccaaaactt caatcttgtc gatataacca ttatttcgtg cccattgaca tatttttttg
     6301 acagtgtcaa attcaccttc cgaaacgcgt gcagaagcaa tttcaatcct atctacacac
     6361 atttcctgca acagcattct ggcaatactc aatttttcct gagctgagaa cgaaactcct
     6421 gaagtttgtt cgccatcacg cagggttgta tccattattt ctaccataaa gcttgctttg
     6481 aaatttcccc aaaaggagaa aaattgaata aaaaaattaa aatttaaaac ttaaaaagtt
     6541 ctggaagctt catatgcttc aatcaaatcc tttttgctaa gcaaatagtc tatatcgtca
     6601 aggcctttta tcagacattc ctttttgtac acattgatat cgaatttttc ggatttgccg
     6661 gttgcattgt tggtaatagt ctgattttcg agatcaaccg ttaaggtcat attgggattt
     6721 tcactgacag attgaaatat ttcgtttaaa aattctggtg taacaactac cggcaatacg
     6781 ccgttgttta atgcgttatt tttgaaaatg tcagcaaaaa aacttgaaac cactacctta
     6841 aatccataac cggctatggc ccatgctgca tgttcgcggc ttgaacctga accgaaattt
     6901 tttcccgcta ccaaaataga acctttatag gtaggattgt tgagaacaaa atcggctttg
     6961 ggacttccgt ccttattgta acgccaatcg gcaaaaagat gatctccaaa accatccttg
     7021 ttggtcgctt ttagaaatct tgccggtata atttgatctg tatccacatt ttcgatagga
     7081 agtggaacaa cagttgatgt cagtttaaca aacttttcca taatatttct tttttaattg
     7141 atttacagtg taatatttat actgatgcca atcaattttt tttgattttt tattaaatga
     7201 ataaattggt tttaagataa aaattctctg ggatcggtaa ttttccccgt aacggcagca
     7261 gcagcggcca ccagtggtcc ggcaaggata gttcgagcgc ccggtccctg acgtccttca
     7321 aagtttcggt tggaagtaga tacggcatat tttccggccg gaaccttgtc gtcattcatc
     7381 gccaaacagg cagaacatcc cggttgacgt aactcaaagc ctgcttccat tagaatttca
     7441 tgcaagccct catcgtgaat ttgtttttcc acttttctgc ttccgggtac cagccaagct
     7501 acaacattgt cggctttctt tttccctttt acaaattgtg caaatgtgcg gaagtcttca
     7561 attcgtccat tggtacagct tcctaaaaat acgtagtcaa ttggtttgcc gagtaatttt
     7621 tcaccgggac gaaatcccat atagttcaat gctttttcaa aagaaattct cccggcttcg
     7681 tccacctgtt ccaaagttgg aatagtttga tcaataccca tgcccatacc gggattggtt
     7741 ccataagtaa tcattggaga aatttcggag gcgtcgaaag tatattcctt gtcgaaaaca
     7801 gcatcttggt cagaatacaa agttttccag taagcaagtt ttttattcca ttcttcccct
     7861 ttgggagcaa attcgcggtt tttcaagtag gcaaaagttg tttcgtcggg tgcaatcatg
     7921 cctccacgtg ctcccatttc aatactcagg ttgcaaacgg tcattcgctc ttccatattc
     7981 atattgcgaa tagcttcgcc ggcatattcg acaaaatatc ctgtcgctcc acctgtcgaa
     8041 agttttgaaa tcatgtaaag tgccaaatct tttgcggtta ctcctttatt caattttccg
     8101 tcaaaagtga tacgcatggt tttgggacgt gtttgcaaaa tacattgtgt agccaacacc
     8161 atttcaactt cgctggttcc aattccaaaa gcgatggcac caaaagcgcc gtgagttgaa
     8221 gtatggctgt cgccgcaaac tatggtcatt ccgggctgag taaaaccatt ttcaggacca
     8281 ataatatgaa ccacaccatt tttttcatgt cccataggga aaaacaaggg tagctgaaag
     8341 tctctggcat ttttctcaaa tgtatccagc tgatttcgtg aaacagggtc tttcaccggc
     8401 tgatcctgat gaagagtagg aatattgtgg tcggcagtca aagtggtttg taacggacga
     8461 aaaactttca atcctcttgc tctcaatcca ttaaaagcct gtgggcttgt aacttcgtga
     8521 acataatgcc tgtcgatata caaaatagta ggcccgtttt caatcgtgga aaccacatga
     8581 gcatcccaaa ttttatcaaa taatgtattc atgttttttt gatttgtgta acgaaaaaag
     8641 attttgtttt cttttgattg acaataaaat aagaatcatt ttttaaatat gtattttaat
     8701 tattttttcg gtaaaataat tttacttttg caacatttga aaacacaaaa atgtttagtt
     8761 gccgaacata attattctac aaatttatta atggcgtcga tatatgcttc aacggaagca
     8821 gcaacaatat cggtgttggc tccaaatcca taataaacca caccttcgtg ctcaacctgc
     8881 atgtgaactt tacccacatc atcgcttcct ttattgatgg actgaatggt aaattcctgc
     8941 aatgtcattt ctcgattgat aatttttttc aaagccttga tggctgcatc aaccggccca
     9001 tttcctgctg aagcggcttc aaatttttca ctgccgataa tgagtccgat acttgctacc
     9061 ggttttaccc ccacaccgga agttacctgt aaatagtcga gttttatacg ttctttctct
     9121 gacctttctt taccggcaag catcagcaaa tcatcgtcgt ttatatcttt tttcttgtcg
     9181 gccaacttca gaaattcttc ataaacagca tccagttttt tatctgccaa ttccacgccc
     9241 aaagtattca gtctgtgttt caaggcggca cgtccgctcc gtgctgtcaa cactattgaa
     9301 ttgtgatcaa tacctacgtc ttctggattc ataatttcgt aattctccct gtttttcaat
     9361 acgccatctt ggtgaattcc cgaagaatga gcaaaagcat ttctaccaac tactgcttta
     9421 ttgggctgaa cgggcatatt cattaaattg gaaaccagac ggctggttga ataaatcttt
     9481 ttggtattga tttgtgtttc gatattcagt tttttatgac atttcaaaat catggcaatt
     9541 tcttccaaag ctgtgtttcc tgcacgttct ccgataccgt ttatggtaac ttcggcttga
     9601 cgtgctccat tcatgattcc cgatatggtg ttggcagttg ccatacccaa atcgttatga
     9661 caatgtgtag aaattatggc tttgtgaata ccatccacat gttccattag atatttgatt
     9721 ttttcaccat attcccaagg gaggcaatat cctgtggtgt cgggaatatt gacaacagta
     9781 gctccggctt tgattacggc ttctaccacg cgtgcaagat actcattttc tgttctaccg
     9841 gcatcttcac aataaaattc cacgtcttca acaaattttt ttgcatattt tactgctgct
     9901 accgagcgtt ccagaatttc ttcctgattg gaattgaact tgtaacggat atgatattct
     9961 gaagttccta tgccggtgtg aattcttttc ttcttggcat attgaagtgc ttctgctgcc
    10021 acttcaatgt ctttttctac ggcgcgggtc agggcacaaa taaccggcca agtaacagtt
    10081 tttgaaattt ctacaaccga attgaaatcg ccgggactcg aaacgggaaa gcccgcttct
    10141 ataatatcga cacctaaatt ttcaagcgct tttgctactt caattttttc gacagtattg
    10201 agttggcaac ctggaacttg ttcgccgtca cgtaaagtcg tgtcaaaaat aaataatctg
    10261 tccatatttt tttgtttttt gaattgtaac tcaaagttgg ttaattttat cattaatata
    10321 ataaagtaaa aaacctttct tacattaagt gagaaaggtt tttcaaataa aatcggttat
    10381 catatctaca taccaaactc acttctgctc ttttgccaaa agtagaataa tgctgcttag
    10441 caaagtatgt agagaattca gtttcatgtt ttgttttaat ttgtaaattc ttcgcaaaga
    10501 tactgcaatt tttctaaatg caaaatataa agtgaaattt ttttctattg ttttgtttca
    10561 attctcaatt ttagcactga aaaaatttac aatttttctt ttttaaaaat gtatattgct
    10621 gatattcaat tcaatttcgc caaattcgat caaagcatta aaaagcctaa tggcagaata
    10681 attttgcaaa tcgctaagtt taatatcttt ttcgaaaatc tttccttctt ctaacaaatt
    10741 agctctatta actccataaa tcaatggttt ggttggcgtt tgccaagaaa aaccgtcaaa
    10801 aagggcaata ttgcaaatag aagtatctgt caataagccg tttttcatca acaaaacgtc
    10861 atcataattt cctctcatgt caaatgctgc gttaaaatct gtacgatctt cctttttata
    10921 gggacgtgat tctatctcgg tatgtactat gcgaagagaa ttaatttcct tttttttgta
    10981 tggtaaatat tctatcttca aaattttaga ttcaaacgta attttgcatt tgaataaacc
    11041 cttttgtggt ggatgtgaat cggttaaagc cgattggaga tgaataaaat tgtcattcgg
    11101 ataaaataat tccgaacaaa tattcattct cttctgatga aatttgaggc gataaaaaat
    11161 tccatccaac agttttatgg attcaataaa tcggtacata cactttttct aacatttctc
    11221 gatattcttt taaaacttcg ctgttcgatg ttattcctcc tccgcttttg aaaatcattt
    11281 gtccgttctt ttgctcaatg aagcgtatca gtaccgctga atccaaattt tttccatcat
    11341 attgcccgaa aattccggta taaaaacctc tgtcatatcc ttcaatttct ctgatgattt
    11401 ccaatgtttt actttttgga gcacccgaaa tcgaaccggc aggaagcatg gaaataataa
    11461 tatttcccaa ataacaacaa taattttcag gtaattctcc tgtaatttct gaactcgttt
    11521 gaaatagttc attttgattg gtaattattt tatcgatgta tctgtatttc ttgacttcaa
    11581 ctttttcggc aaccatactt aaatcattac gcataagatc tacaatagtt gcatgttcag
    11641 cttgttcttt gaagtcgttc aaaatcgcat cttcggcatt tgcaacagaa gcatcaatag
    11701 ttcctttcat gggaaaagtg gaaataattc catcctcaat ttttataaaa gtttcaggcg
    11761 aaaaaacggt aaataaatct ttgagccata gtttgtattt tgaaacactt aactcataca
    11821 attcaagcaa atttaagttg gtacttactt ctgttggctg cgttaaattc agtaaaaaag
    11881 aatttccgca tcggatattt tccattacaa aatgaaattt caatttgtat tcatcaaaat
    11941 ttacgggatg aatattccat tgaaaattaa atgaattttt tgtcgatttt tcttgattgg
    12001 aaaaactttc atccgacaat tgaaatcgta tatattggtc gtccagatct tgatttttnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca agcccaacaa atttattaat
    12181 ttgttgtaaa tttgcgccgt tcatttacga aattaaataa atattcattt tatactgaca
    12241 accatgaaaa tccgtcaact ttccgttttt gtagaaaaca aaacaggaca tctcaatcag
    12301 attttagcgg ttctggcacg caacaacatt aacattattg ccctcacgct ggccgatagc
    12361 attgattttg gacttcttag aatgctggtt tccgatgctg aaaaagcagc cgaagccctt
    12421 cgcaccgaaa attttacagt tcgcattcat gaagttttat cgctcgagct ggaaggtgtt
    12481 ccgggttcat tatcgcgggt attgaaccat ttttctgaca acggaattgc catcgaatat
    12541 atttatgctt tcagttttgg ctccaaatcc atattggtaa tgcgtcccga caacaccgaa
    12601 aaagccatcg aaataattca aaaatatcaa ttaaaaccca tatacgaatc cgatttaaaa
    12661 gcacaattat gatttggaac gaagagatcg aatgcatgag tagcagtgaa atgcgaaagc
    12721 tgcaaagtga aagattgaaa gaattggtca acaaagttta tcaaaatgtg gaattttatc
    12781 gaaaaaaaat ggatgaaaag ggtgttagac cttccgatat tcgttctatt gatgatattt
    12841 ccaaattacc ttttaccaat aaaaccgatt tgcgcgacaa ttatccattt ggattgtttg
    12901 ctgctcccat gaaggaaatt gtgcggattc atgcttccag cggtactaca ggcaaaccaa
    12961 caactgttgg atataccaaa aaagatattg aaaactggca ggaagtactt gcccgatgtc
    13021 tgatcatgtc gggagttacg cctgaagata ttgttcaggt ttcatttggt tacggactgt
    13081 ttacgggcgg attgggaatt cattacggtg ccgaaaatgt aggttgtacg gttgtcccga
    13141 tttcggcagg caatacacga cggcaattac aactcatgtc cgattttggt tccactgtgt
    13201 tagcctgtac tccatcgtat gcacttcatt tggctgatgc tatgtcggaa tttggttatt
    13261 cgctctccga catgaaactt aaaacgggtg tatttggagc ggagccgttt tctgagaata
    13321 tgagacttga attggagaaa aagtggggaa ttaaagcttt tgacatttac ggattaagtg
    13381 aaattatggg accgggagtt gccaatgatt gcgagtttca tgatggttta catattcatg
    13441 aagatcactt tttccccgaa gtggtcgatc ccaaaaccaa agagccttta cccgacggcc
    13501 aacaggggga attggttttc accaccttga ctaaggaagg aattccattg ttgcgctaca
    13561 atacacacga tttgaccaca ctgaatcgag aaagatgtga atgtggacgt acattggtaa
    13621 gaatgaagaa aattactggt agaagtgatg atatgctgat tatcagaggt gtaaatcttt
    13681 tcccatcgca aatcgaacag gtgctgctcg aaatgcgcga aaccagtgca cattacatgt
    13741 tatatgttga tagagaaaac aatctcgata ttcttgaact gaaagtggaa ctggacgaaa
    13801 ctaaaatggt agataccata aaggatttga tgtctctttc cagaaaaata gaacattcgc
    13861 ttcacagtgc acttggtttg agcgtaaaag ttacgcttgt tgagccgaaa agtattccga
    13921 gaagtgaagg aaaagctgta agagtgatag ataatcgaaa aatttaatgg tcttaaatta
    13981 aaaaacatgt caatttctaa agatttggtg gatgagattt tcagacaatt tggacaggaa
    14041 gatataaata aactctccat tcgtgaaatg gggaaacttg ttgctcaaat tgaacaaaaa
    14101 accaatgtag aatttatcca catggaaatg ggtgtgcccg gtcttcctcc tttgcaaaca
    14161 actgtagatg ctgaaaagga agcattggac aggggattgg ctgctcttta tcccgatatt
    14221 gaaggaattc cttttgtgaa aaacgaaacc gcacgttttg caaaattatt catggacatt
    14281 gatgttactc ctttgaactg cattccaacc gtaggagcca tgcagggaag ttttgccgct
    14341 ttgctggctg ctgcccgcac caacaaaaat aaaccctgta cgttgtttat tgatcccgga
    14401 tttccggtaa acaaatcgca actgcaactc ataggacttc cattcaaatc atttgacatt
    14461 cttgaaaata ggggtgaaag acttcgttct gcattaactt catttttaaa agaaggagat
    14521 atttgcacca ttacttattc ttcgcccaat aatccttcat ggatcaattt tacagaagaa
    14581 gaattgcaaa ttataggcga attggcaacc gaatatgacg ttattgttgt cgaagatttg
    14641 gcttattttg gcatggattt cagaaaagat tacggtagac ccggtgtgcc gccttatcag
    14701 ccaactgtag ctcgttatac cgaaaactac attttgttgc tttcagcttc aaaaattttt
    14761 agttatgccg gacagcgaat tggcatgatg attatttccg agtcgttgcg tacccgtcat
    14821 tatccggaac ttaaaaacta ctttaccagc gacgttttgg gacattttat tccttatgga
    14881 gcgctgtatg ctacgacggc aggtacatca cacacagctc aatatgcatt tgcagtaact
    14941 ttaaaggcag tgaatgatgg aaaaataaat tttctggaag ctttgaaaaa atatgccgaa
    15001 aaagcaaaaa taatgaagga aatatttctc aaaaatggat tttacatcgt ttacaaagaa
    15061 aaaaatgaag agctggcaga tggtttttat tttactgtgg cttatccgga gatgacgagt
    15121 agccagttga tgaagaattt tctttattac ggtctcagtg ctatttcact ctccattaca
    15181 ggtagtaagg ataatcacgg tttacgtata tgtgtttcat ttgtccgacc agatcaaatt
    15241 cctgtccttg acaaacgctt gaaagaattt gccgaagaca tgaaaaaaaa cgtttaattt
    15301 ttttcttcta caacttggag caaaaatctg atgattttgg aaaagtgaca tttgtattat
    15361 ttacttcaat ttgacactct ttcacaagtt atgtccgaaa agatgatcga gtaggtaggg
    15421 gaattactcc cccaccctcc catcaaatcc tgcgttaagg ctcgtttctg ttcgcctgct
    15481 tcttttactt gc
//
LOCUS       DAOSIB010000055        15433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_3986_length_15433_cov_11.134738, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000055 DAOSIB010000000
VERSION     DAOSIB010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15433
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3986_length_15433_cov_11.134738"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            445..1143
                     /locus_tag="PLD44_06080"
     CDS             445..1143
                     /locus_tag="PLD44_06080"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOF98936.1"
                     /translation="MTSNIESERVLEKQRWKFAIILPSIVCILWILCFVLERGMGWDF
                     HKAGIYPRKLENIWSIFTYSFIHADWKHLMNNVLSFFILGICLYYFYSEVANRVLIIS
                     AVLSGLILWCIGRENFHIGASGIIYSLAFFLFFSGIIRKHIPLIAISLIVVFIYGSMV
                     WHLFPWLPDDPVSWEGHLSGAATGIVLAFVYRHKGPQPPKPEWEEEDDDEDNEQEDQY
                     DWKENYEQEQWKEE"
     assembly_gap    1248..1347
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2070..3545
                     /locus_tag="PLD44_06085"
     CDS             2070..3545
                     /locus_tag="PLD44_06085"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOF98937.1"
                     /translation="MKQKFLSILFLFVNYSFLISAQNNLSTNDTGEVQTIKVIDLDSL
                     LKNTKWIQNNETNSEIIELSEDSDTLKTKHLEKIKVLKDSFQLFVSPTALATIKSDSL
                     LYLSNPFFIELVYMGLPFDFDIKPKEDLLTLFHERKASTLEENAYRPIKVPTSQESLI
                     KLRQDARNEITRTAVGLYKRMFYELSNPYVKGNRPIQVKPIEKVEFVDKNKYVNGKER
                     KMHVPEFQVGPWRHKGSFLLQFSENYISKNWYQGGNSNLSVLGILSGQINYDDKKRTQ
                     WENSLEWRSGFYSVFGDTTALRTLNTNEDVLKINSKFGYKASGNWFYSGAIDFSTQFL
                     KNFKSVNSNELKASLFTPIRLNVNVGMDYKYKKIFSLMVSPLSLKYIYLSDTVKVNPN
                     LFGIQKGKNHLEQIGSSFKAVVSYAPSREIQLDSKFAFYTNYEKIEVDWEIVCNMIVN
                     RFVSTRISINPRYDNTMIMAEGERAKVQFKQLLSVGFSHKF"
     gene            complement(3555..4091)
                     /locus_tag="PLD44_06090"
     CDS             complement(3555..4091)
                     /locus_tag="PLD44_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOF98938.1"
                     /translation="MHPFSIIEKYYVKDSPLYRVLVIHSQQVRDKALEVAKQHPELNI
                     DTEFVGEACLLHDIGIFLCHAPRIYCFGEHEYIEHGYLGSDLLKKEGLPQHALVAERH
                     TGTGFTKEEIRLKKLPLPDRDMLPLSLEEQLICYADKFYSKSKLNLQLSVEEIRQNLS
                     FYGKEKVEKFDSWHQLFG"
     gene            complement(4242..5483)
                     /locus_tag="PLD44_06095"
     CDS             complement(4242..5483)
                     /locus_tag="PLD44_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOF98939.1"
                     /translation="MEGMKNFRYKIGFLALLLISLFPISIFAQSSGSENLVIESLQKF
                     WNNTGLANFEFKYLAMIVVGLVFIYLGIAHKWEPLLLVPIGFGILIGNVPFTDGFRIG
                     IYEQGSVMNILYSGVVQGWYPPLIFLGIGAMTDFSSLISNPKLMMLGAAAQLGIFVSF
                     IIAYAVGFSPAQSGAIGIIGGADGPTAIFISSRLAPELMGAIAVAAYSYMALVPVIQP
                     PIMKLLTTNKERKIRMKPPRQVTKLEKIIFPIIGLLLTTFISPSAMPLLGMLFLGNLL
                     KESGVTERLADTARNALLNSITILLGVTVGASTQADRFLTVDSLMIFGLGAFSFMIAT
                     AGGVLFAKIMNLFLKEGNKINPLIGSAGVSAVPDSARVSQDVGLSYDKSNHLLMHAMA
                     PNVAGVIGSAVAAGILLGILS"
     gene            complement(5485..5931)
                     /locus_tag="PLD44_06100"
     CDS             complement(5485..5931)
                     /locus_tag="PLD44_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOF98940.1"
                     /translation="MKKFDFNINGNDFHVYIKSIDGNIAEIEVNGTPYTVTLNQEVKV
                     SKTPILVRKEVPTTPSNMNVADKFNPVPSNQKPSSTVIKSPLPGNILRINVKEGDTFK
                     EGDVLLVMESMKMENNILAERNGKIVKVCVSVGKAVLQDEVLFEIE"
     gene            complement(5931..6278)
                     /locus_tag="PLD44_06105"
     CDS             complement(5931..6278)
                     /locus_tag="PLD44_06105"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HOF98941.1"
                     /translation="MIMKLLMINSWEELLTAAAIGIMVVFSVLFVLVLLFELFQVIVL
                     KVAKKKVVKSGIDVDKIKDIPANEIAAISMALYLYLDSMHDEESNVLTIKRIERRYSP
                     WNSKIYGLNNLHR"
     gene            complement(6282..7841)
                     /locus_tag="PLD44_06110"
     CDS             complement(6282..7841)
                     /locus_tag="PLD44_06110"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOF98942.1"
                     /translation="MNNLEKLEQLISLRAKAKLGGGEKKIESQHKKGKYTARERIEML
                     LDDGSFEELDMFVTHRCTNFGMENEKYLGDGVVTGHGTIDGRTVYVYAQDFTVFGGSL
                     SEMMAQKICKVMDMAMKMGAPIIGINDSGGARIQEGVTSLAGYAEIFERNILASGLVP
                     QISAIFGPCAGGAVYSPALTDFILMTEENSYMFVTGPKVVKSVTGEDITTEDLGGANV
                     HSSKSGVSHFKVESEEEGLLMIRKLLSYLPQNNLEEPPLIQNYDPIDRHEDKLNTIVP
                     DNPNLPYDMKEIIHSLVDNGEFLEVHQFYARNIIVGFARLNGASIGIVANQPNFLAGV
                     LDCDASRKAARFVRFCDAFNIPILTLVDVPGFLPGSGQEYSGIIIHGAKLMFAYGEAT
                     VPKVTVTIRKSYGGAHDVMSCKQLRGDFNYAWPTAEIAVMGASGAIEILEGKNIANIA
                     DPEEKAKYIAEKVAEYNEKFSNPYQAASYGYIDDVIEPRNTRFRIIRAFETLQTKKVV
                     NPLKKHCNIPL"
     gene            complement(7878..8294)
                     /gene="mce"
                     /locus_tag="PLD44_06115"
     CDS             complement(7878..8294)
                     /gene="mce"
                     /locus_tag="PLD44_06115"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOF98943.1"
                     /translation="MKPKKIEHIGIAVKSLETAIPFFESVLGLTCYAIEEVKDQKVKT
                     AFFKVGDTKIELLESTDPEGPIGKFIEKRGEGVHHIALAVENVQQALNEASDKGVMLI
                     DKQPRKGAEGLNIAFIHPKSTFGILTELCENPNINC"
     gene            8960..9652
                     /locus_tag="PLD44_06120"
     CDS             8960..9652
                     /locus_tag="PLD44_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOF98944.1"
                     /translation="MKKLFLLSIMMVFTFFSFQSGQNKKGVEKSQNTLPMDLKTFDTI
                     QLLAPDTMGGKSLMQTFQHRKSDREFKQTPLSLKHLSEILWAAYGINRPDGKRTVPSA
                     RALYPLQVYAVLPNGIYRYEPQTHRLVPVVKGDYRKLAGLQDFVYTAPMNLIFIADFN
                     KYNLTQKEPDANSIRMVSLDAGHCTQNVYLYCASEGLRCVVRAGAKADELLSLLSLDS
                     HYRFIVAQTVGY"
     gene            complement(9682..11007)
                     /locus_tag="PLD44_06125"
     CDS             complement(9682..11007)
                     /locus_tag="PLD44_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOF98945.1"
                     /translation="MNWKQLITTKRLGMEAYHEKRQDNRSEFQRDYDRLIFSPPFRRL
                     QNKTQVFPLPGSVFVHNRLTHSLEVSSVGRSLGINVARQIKEKNNLPDAFIDEVGSIV
                     SAACLAHDMGNPPFGHSGEKAIATYFSEGEGQILQEKMLPEEWSDLISFEGNANTFRV
                     LTHQFRGRRPGGFVLTYSTLASIVKYPYASVFSDPQRQKFGFFHSEKSDYEKIAHELG
                     IPHSFSQPDKYVRYPLVYLVEAADDICYQIMDIEDAHKLKILSTDETKALFLSYFEGK
                     KLERKLQTIAMVDDVNEQIAYLRSSVIGQLIDNCSSVFLENEPQILEGSFSSTLIKLL
                     PPVSLKAYQNCSKTAFEKIYCSSEVLDVELAGYKIIFTLLENLVKAVLSPEKAYSQQL
                     LMRIPKQYEIDQPTTYGKVMAILDYISGMTDVYALDLYRKITGMNIPTL"
     gene            complement(11059..11793)
                     /locus_tag="PLD44_06130"
     CDS             complement(11059..11793)
                     /locus_tag="PLD44_06130"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOF98946.1"
                     /translation="MNTSSSQRKISDPELGDIYFNRSQRATRINIQILEDKLKVTLPM
                     RASEKDAMRFIDSVREKILKKRAQVKEKIRKNKQIIDEQSPFRALTFVTDIKPTHRED
                     IYCCLKNQVLSIEIPSEVDCREEQMQNYLHRIIARYLRLEAKRVLPQRTRLLADKFGF
                     RFSTVKIQSSRTRWGSCSAKGNINLSLYLMALPAHLIDYVILHELCHTRELNHSTDFW
                     RWLNRVTDNQSDRLKEELKNFNFNWD"
     gene            complement(11975..13198)
                     /locus_tag="PLD44_06135"
     CDS             complement(11975..13198)
                     /locus_tag="PLD44_06135"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOF98947.1"
                     /translation="MAYINENFIKIPPTYLFSEIAKRVEQHKAENPSAKIIRMGIGDV
                     TLPLPDASVDAMIKAAEEQRHAETFRGYGPEQGYEFLRQAIVENDFRARGIKLDVDEV
                     FVSDGAKSDTGNIGDIFGIDNCVAITDPSYPVYVDTNAMAGRAGEPNEFNEWNGLTYL
                     PCNEANNFVPQLPDKKVDMIYLCYPNNPTGTTLSKQQLQVWVEYAKANRSVILFDAAY
                     EAFITEENVPHSIYEIEGAKEVAIEFRSFSKTAGFTGTRCGYTVVPKELVGYTHQGEA
                     VALNKLWLRRQSTKFNGTSYIVQRGAEATFTPEGRKQVKQLIEYYLGNARIIREGVAK
                     LGLKVFGGVNAPYVWVKAPDNMKSWEYFDFLLKQKNIVTTPGAGFGRSGEGYIRFSAF
                     GSRENTIEAMERLIK"
     gene            complement(13277..14110)
                     /gene="dapF"
                     /locus_tag="PLD44_06140"
     CDS             complement(13277..14110)
                     /gene="dapF"
                     /locus_tag="PLD44_06140"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOF98948.1"
                     /translation="MKFTKMHGIGNDYIYINGFEEQLENPGELSIRLSDRHKGVGSDG
                     LVIILPSSKADFRMQMFNADGSEAEMCGNAARCIGKYVYEKGMTDKTELTLETLAGIK
                     TLQLFLDERNNVVSVTVDMGKPVLEAEKIPTTLPGKQIVNFPVSFDGINYAITCVSMG
                     NPHTVIFTENIANMDIEKMGKKIENAPIFPRRTNVEFIEVIDKSRIEMRVWERGSGET
                     MACGTGACASVVAGVLNGLVSRKATVELLGGNLEIEWNENDEHVYLTGPAETVFEGEI
                     S"
     gene            complement(14301..15326)
                     /locus_tag="PLD44_06145"
     CDS             complement(14301..15326)
                     /locus_tag="PLD44_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HOF98949.1"
                     /translation="MKCFASDNYSGIHPDILKAIVEANADHEISYGDDTYTQRAKKLF
                     QQKLGNVEVLFVFNGTGANIVCLKCCTLPFQSIVCAQTAHILTDECGAVFQNIGCSIM
                     PVHTPDGKLTPELISPVLVHKGNVHSAQPKVISISQTTEVGTLYDTEELKALCDFAHK
                     NNMLVHVDGARISNAVAALGKSLKEVTVDCGVDIMSFGGTKNGMMMGEAILIFNDELN
                     ANARFYQKQSLQLYSKNRFIAAQFMALLKNDLWLKMATHSNKMASLLAEKMRAIPEVQ
                     ITLPVQANAVFAIIPEKVIEPLREKFRFYTWDFQTNELRWMCSFDTKPEEIDEFAETL
                     KKLLADR"
BASE COUNT         4873 a         2940 c         2682 g         4838 t
ORIGIN      
        1 aaagagaccg ttcaaggtta attaacaagg atttgtcgaa acttagataa ctcatctgat
       61 tgttattctt agactatctg attatcaatt aaatacaatt ttcttttaaa aaggaatgta
      121 aattagaaac attacaaaaa tcaatcctta gatagatttt aatattgctt gcaatttatc
      181 gaaaatttcc aattaaaaaa aaggaagttg ataatttttt caaggtgacg aaaaaaattt
      241 cttggttccc aacaaatcca aatttcattg atacatgcaa tttgttgttt ttcaaatttt
      301 tataaaattc atcaaaaaaa atgatggctg aatcgtagag aaagttcatt tgacagttaa
      361 aaattatctt ctcaaacaaa aaaattttta cctttgatgt aatttttagt gtttggaaga
      421 cgttatttaa aacagagttg cgaaatgacg agcaacatcg agagtgaaag agttctggag
      481 aagcaacggt ggaaattcgc cattattttg ccttcaatcg tatgtatttt gtggatatta
      541 tgttttgtat tggagcgcgg catgggttgg gattttcata aagccggcat ttatccgcga
      601 aaactcgaaa acatttggag tatatttact tacagtttca ttcatgccga ttggaagcat
      661 ttaatgaata acgttctttc tttttttatt ttgggaatat gcctgtatta tttttacagt
      721 gaggttgcca atcgagtgct aatcatatcg gccgtgctca gtggattgat tttgtggtgt
      781 atcggacgcg aaaattttca cattggcgcc agtggcatta tttactcgct ggcatttttt
      841 ctgtttttca gcggaataat cagaaaacat attcctttga tagctatttc gttgattgta
      901 gtgtttattt atggtagtat ggtttggcat cttttcccct ggttgccgga cgatccggtt
      961 tcatgggaag gacatttatc gggagccgca accggtatcg tgctggcatt tgtgtacagg
     1021 cacaaaggcc cacagccacc caaacccgaa tgggaagaag aagatgatga tgaagataat
     1081 gaacaagaag atcaatatga ttggaaagaa aattatgaac aagaacagtg gaaagaagaa
     1141 tgatataaca atcaattttg acaaacaata attttaatca atatggacga gaaaaataca
     1201 aaagaaatta atttgctgga tttgataaaa atgttcaatg cttgggtnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnntgc ctcgaacaat tgcaaaaggt ggttcaattt
     1381 tttagaaaat aaaaatacct tttctgtaca ttaaaaaaac aatataccta attttgaagt
     1441 agagcacaat aaatttattt atacaattat attttacttg ttttcaaaaa tatagtttat
     1501 acaagctttc ataccacttc gtagcaggaa caaaaacttc ttttgatttt aacaaaagaa
     1561 ttccccgcga aaactctttt atagagcatt ttagtactta aagggacttt tgtgcaataa
     1621 ttttttcaaa acgttaacgg ctacaaaata agcctcgtta cgaatgtaaa aaatctcatc
     1681 accggtaatt tttatattca agaacggtaa cgagtaacac aaacttaccg gtattgttat
     1741 aaatatactt gcctgcagaa aaaaatattt ggacgcaggg tgaagtctaa ggctctggtt
     1801 aagggcaaaa ttctttcctt taaaacctca aatccgatta aaaactaaat ggattattca
     1861 tctccaaatg ctcactgcta atttaccaag aaaaattttc actgcaatct aaatgagttt
     1921 tgatatgaat tttacagatg atacgccgat gttatcttcg caaagttgct gtttgctgtt
     1981 ataaattaca ataggtcatt atcaagataa tcattttttt tttaattttg ttgaacttta
     2041 ccaatcaaaa agacgaataa agaaacatca tgaaacaaaa atttctgagc attttatttt
     2101 tatttgtaaa ctattcattc ctcataagtg cacaaaacaa cttatcgaca aacgatactg
     2161 gtgaagttca aacaatcaaa gtaattgatt tggacagttt gctaaagaat acaaaatgga
     2221 tacaaaacaa cgaaacaaat tcggaaatca tagaattgtc agaagattca gatacattga
     2281 aaacaaaaca tttagaaaaa ataaaagttc tgaaagattc tttccaactg tttgtttctc
     2341 caactgcttt agctacgatt aaatcggatt ctttgttata tttatccaat ccctttttta
     2401 ttgagctggt ctatatgggt cttccttttg attttgatat aaaacctaaa gaagatttac
     2461 tcacattatt tcacgagcga aaagcttcca cacttgaaga aaatgcttat cgacccataa
     2521 aagtacctac ttcacaagaa tcgttaatta aattgcgtca ggatgcaaga aatgaaatta
     2581 ccagaacagc cgtcggatta tataaaagga tgttctacga gctctcaaat ccttatgtaa
     2641 aaggtaatcg gcctattcaa gtaaaaccta tagaaaaagt tgagtttgtt gacaaaaata
     2701 aatacgtgaa tggaaaagaa agaaaaatgc atgttcctga atttcaagtt gggccttggc
     2761 gacataaagg ttcatttctg ctacaatttt ccgaaaatta tatttcaaaa aactggtatc
     2821 agggaggaaa cagcaatctt tcggtattgg gcattttaag cgggcaaatt aattatgacg
     2881 acaaaaaacg aacgcaatgg gaaaacagtc tggagtggag aagcgggttt tattcagttt
     2941 ttggtgatac tacagcactg cgaaccttaa acaccaatga agatgttttg aaaataaaca
     3001 gcaaatttgg ctataaggca agtggcaatt ggttttatag tggagcgata gatttctcta
     3061 ctcaattttt aaaaaatttc aagtcagtca attctaacga attaaaagct tcactgttta
     3121 cgccaatccg tttaaatgtc aatgttggta tggattacaa gtataaaaag attttttcgc
     3181 tcatggtgtc tcccttgtcc ttaaaatata tatatctaag cgacactgtc aaggttaacc
     3241 ccaatttgtt cgggatacaa aaaggaaaaa atcatttaga gcaaattggt agttcgttta
     3301 aagcggttgt ttcttatgca ccatcaagag aaatacagct cgattccaag tttgcttttt
     3361 atacaaacta tgagaaaatt gaagtcgatt gggaaatagt ttgcaatatg atagtaaatc
     3421 gttttgtctc tactcgtatt tcgatcaatc cgcgttacga caacactatg ataatggcag
     3481 aaggcgaaag agcgaaggta caattcaaac aactactcag cgtaggattt tcccataaat
     3541 tctaacaaac cggattaacc aaaaagttga tgccaagaat caaatttctc gactttttcc
     3601 ttcccataaa agcttaaatt ttgcctaatc tcttctaccg aaagctggag attcaatttt
     3661 gatttggaat aaaatttgtc ggcatagcat attaactgtt cttcgagtga aaggggaagc
     3721 atatcgcgat cagggagagg aagtttcttg agtctgattt cttctttagt aaaacccgtt
     3781 ccggtgtgtc tttcggcaac aagagcatgt tgtggaagcc cttctttttt caataaatct
     3841 gaacccaaat aaccgtgttc gatatattca tgttctccaa aacaataaat tcgtggtgca
     3901 tgacagagaa aaattccgat gtcgtgcagc agacaagctt cccccacaaa ttcagtgtcg
     3961 atgttaagtt ccggatgttg cttagctact tctaatgcct tgtctctgac ttgttggcta
     4021 tgaataacaa ggacacgata aagaggagaa tctttaacgt aatatttttc gataattgaa
     4081 aaaggatgaa taaccataat aaattgttaa aaaagttttt tcaatcaatt cataaaaaat
     4141 aacaagagaa tatacagttt atttcaaaaa aacttatcaa ataaaaagcc cactgttaaa
     4201 aacacaatga gcttatttgt aaaaaaatca aaatcaaaca tttaagaaag tatacccagc
     4261 aaaatacctg ctgccacggc cgaaccaata acaccagcaa cattgggtgc cattgcatgc
     4321 attaataagt ggtttgattt atcgtaagac aaaccgacat cctgtgaaac cctcgcagaa
     4381 tcaggaacag cagaaacacc ggcagaaccg ataagaggat tgattttgtt cccttcttta
     4441 aggaaaagat tcataatctt ggcaaaaaga acacctcctg cagtagcgat cataaatgag
     4501 aaagctccca agccaaaaat cataagcgaa tcaacagtga gaaaacgatc ggcttgagta
     4561 gaagcaccaa cagttacacc taacaatatg gtaatagaat tgagtaaagc attgcgagcc
     4621 gtatcagcca aacgttcagt aacaccggat tctttcaaaa gattgcccaa aaacaacatt
     4681 cccaacaaag gcatagcgct gggcgaaata aaggttgtca acaacaagcc gataatgggg
     4741 aaaataattt tttccaattt ggtaacttgt cggggaggtt tcattctgat ttttcgctcc
     4801 ttgtttgttg ttaaaagttt cattataggt ggctgaataa caggaaccag ggccatataa
     4861 gaataagcag ccaccgcaat agctcccata agttcgggcg ctaaacgaga agaaataaaa
     4921 atagctgtcg ggccatcagc acctccgata attccaattg ccccagattg ggcaggtgaa
     4981 aatcctactg cataagcaat aatgaaagaa acaaaaattc ctaattgcgc agcagcacca
     5041 agcatcatta acttaggatt ggaaatcaga gaagaaaaat cggtcatagc accaataccc
     5101 aaaaagatca agggtggata ccatccttgt acaactccgc tgtacaaaat attcattacc
     5161 gaaccttgtt cataaattcc gatgcggaaa ccgtcagtaa aaggaacgtt tccaatcaga
     5221 atgccaaaac ctattggtac gagaagcaaa ggttcccatt tgtgtgcaat acccaaataa
     5281 atgaaaacca aacctaccac aatcatagcc aaatacttaa attcaaaatt ggctaatcca
     5341 gtattgttcc aaaatttctg caaactctct atcaccagat tttcactgcc tgacgattgt
     5401 gcaaaaatac tgatggggaa caaacttatc aacaataggg caagaaaacc aattttatat
     5461 ctaaaatttt tcattccttc aatattattc gatttcaaat aaaacttcat cttgtaaaac
     5521 ggcttttcct accgaaacgc aaacttttac aattttacca ttgcgctcgg caagaatatt
     5581 attttccatt ttcattgatt ccatcaccag caaaacatcc ccttctttga atgtatcccc
     5641 ttcttttacg tttattctta aaatatttcc cggaagtgga gattttatta ccgtagagct
     5701 gggtttttgg ttagaaggaa cgggattaaa cttatcagca acattcatat tactgggagt
     5761 tgtgggcacc tcttttctta caagtatagg agttttgctc actttcacct cttgattcaa
     5821 tgttacggtg taaggtgtac catttacttc aatttcagca atatttccat ctatagattt
     5881 tatataaaca tgaaaatcgt taccattaat attaaaatca aattttttca ttatcggtgt
     5941 aaattattta gtccataaat ttttgaattc catggcgaat aacgcctttc tattcgttta
     6001 atggtaagca cattgctttc ttcatcatgc atactgtcta aatataaata taacgccata
     6061 gaaatggctg caatttcatt ggcgggaata tcctttattt tatcgacatc tatacctgat
     6121 ttcactactt tcttttttgc cacttttaaa actattacct gaaaaagttc aaaaagcagt
     6181 accaaaacga ataagacgga aaaaacaacc attataccta ttgcagctgc tgtaaggagt
     6241 tcttcccatg agttgatcat caataatttc attatcattt tttataaagg aatattacaa
     6301 tgttttttta atggattcac gacctttttt gtttgcaaag tttcaaaagc acgaatgatt
     6361 ctgaaacgtg tattgcgagg ttcaatcaca tcgtcaatat agccataaga agcagcctga
     6421 taaggatttg aaaatttttc gttgtattca gcaacttttt cagcaatata tttggctttt
     6481 tcttcgggat ctgcaatatt agcgatattt tttccttcga gaatttcaat ggcccctgaa
     6541 gcacccataa ctgcaatttc ggcagtaggc catgcataat taaaatcacc acgcagctgc
     6601 ttgcaactca ttacatcatg tgctccacca taggattttc ggatggttac tgtaactttg
     6661 ggcacagtag cttcaccgta agcaaacatt aatttggcac catgaatgat aattccggaa
     6721 tattcttgac cggaacctgg taaaaaaccc ggcacatcta ccaatgtaag aataggaata
     6781 ttaaaagcat cacaaaaacg tacaaagcgt gcggctttac gcgaagcatc acaatcaagt
     6841 acaccggcaa gaaaattggg ctgatttgca acaataccta ttgaagctcc atttaagcgt
     6901 gcaaatccta ctatgatatt tcttgcataa aattgatgaa cttccaaaaa ttctccgtta
     6961 tctaccaatg aatgaataat ttctttcatg tcgtaaggca aattcggatt gtcaggtacg
     7021 atggtattca gtttgtcttc atgacgatcg attggatcat agttttgaat caatggaggt
     7081 tcttcaagat tattttgagg taaatacgat aataatttac gtatcatcag caacccttcc
     7141 tcttcgcttt caaccttaaa atgagacact cccgactttg aagaatgaac attggcgcct
     7201 ccaagatcct cagttgtaat atcttcaccc gtaacagatt taacaacttt gggaccggta
     7261 acaaacatgt aactgttttc ctctgtcatc aaaataaaat cagtaagtgc cggtgaataa
     7321 acggctcctc cggcgcaagg gccaaaaata gctgaaattt gtggtacaag acctgaagca
     7381 agaatgtttc gttcaaaaat ttcggcatat ccggccagtg aagttacccc ttcctgaatt
     7441 cgtgcgcctc ccgaatcatt gattccgata attggagcac ccatcttcat agccatgtcc
     7501 atcactttgc aaattttttg tgccatcatt tccgaaagtg aaccaccaaa aaccgtaaaa
     7561 tcttgggcat aaacatacac tgttcgtcca tcaattgtac cgtgtccggt tactacacca
     7621 tctcccaagt atttttcgtt ttccatccca aaattggtac aacgatgtgt aacaaacata
     7681 tcaagctctt caaaacttcc atcatcaagc aacatttcga ttctttctcg cgctgtatat
     7741 ttgccttttt tgtgctgtga ttcaattttc ttttctcctc cacccagctt ggcttttgca
     7801 cgtaaactga ttaattgttc taatttttcc agattattca ttttttcttt ttatcttaaa
     7861 gattttttaa aaataaatca acaatttata ttgggatttt cacacaactc agtcaaaatt
     7921 ccaaaagtag attttggatg tataaaagca atatttaaac cttcggcacc tttgcgtggt
     7981 tgtttgtcta tcaacataac tcctttatcg gatgcttcat ttaaggcttg ctgaacattt
     8041 tctactgcta aagcaatatg atgaactcct tctccgcgtt tttcaataaa tttaccaatt
     8101 ggtccttcag gatcagttga ctccaacagc tcaattttag tgtctccaac cttaaaaaaa
     8161 gctgttttaa ctttttggtc tttcacttcc tctatagcat aacatgtaag tcccaatacg
     8221 ctttcaaaaa aaggaatggc agtttccaaa ctttttacgg caatgccaat gtgttcaatt
     8281 ttttttggtt tcataaaatg aaaaatatag ttgtgtgtta aattatttta ttgataatca
     8341 ataaaataaa attaaaaatt gtaattacag aaaggtcaat tagcgaaata tttccttatt
     8401 taatattttc tgcaaagtta acaatataat tgtttttcaa agaataattt caatggaaaa
     8461 aacatagata tttaaaagtt tttgacaata aaattttttt tcttttaatt tactcttttt
     8521 accagtttca tccgaattaa atattaacca ttttttcaaa gcaaatagaa atcaaacttc
     8581 gaagagccct ccatagtttg gaaaacaacg cgtacctttt ttaaaaacaa tttttttcct
     8641 tttggaaatt ttagattatt gcaatttcag agccttttgc actaaacaac tgatttgtct
     8701 tttttgatag ccaaccctat gcctttttat ttgaatcttt gttgtatttt tacccaaatt
     8761 ttgtaacttt gttctacggt taaatgtttc tttgtttgta attttttagt gaaaagaaac
     8821 catagaaaac cataatctaa gtgggcaaac cggaataaat cttcttcttt tttatcttga
     8881 aatgattttc cttgattttt tattccccta aaaaattgca tttactgatt gaatttattg
     8941 atcaaaaaaa atgaatgtta tgaaaaaact ttttttactg tccatcatga tggtttttac
     9001 attcttttcg ttccaatcgg ggcaaaacaa aaaaggcgtg gaaaaatctc aaaacactct
     9061 gcctatggac ttaaaaacat ttgacactat tcaattgctt gcacctgata caatgggtgg
     9121 aaaatctttg atgcaaactt ttcaacatcg caagtcagat cgggaattta agcaaacgcc
     9181 attgtcattg aagcacttgt ccgaaatact ttgggcagct tatggcatca atcgtcctga
     9241 tggaaaaaga acggttccct cggcaagagc tctttatcca ctgcaagttt atgcagtact
     9301 tcccaatgga atttatcgat acgaaccaca aacacatcga cttgtgcctg ttgtaaaagg
     9361 agattacaga aaattggcag gcttgcagga ttttgtttac acggctccga tgaatttgat
     9421 ctttattgcc gacttcaaca aatacaattt gacgcagaaa gaacctgacg cgaatagcat
     9481 acgaatggtt tcgctcgatg ccggacattg tacccaaaac gtttatttat attgtgcttc
     9541 tgaaggattg aggtgtgtgg ttcgtgccgg agcaaaggca gatgaactgc tttctctgct
     9601 aagtttggac agtcattatc gctttattgt tgctcaaacg gtgggctatt gaccataaaa
     9661 ataaaggtag tctttctgtg ttcagagcgt tgggatgttc attcccgtaa tttttctgta
     9721 aagatccagt gcataaacat cggtcattcc ggaaatataa tccagaatgg ccattacttt
     9781 tccgtaagtt gtcggctgat caatctcata ctgtttggga atgcgcatga gcaattgctg
     9841 agaatatgct ttttcgggac tcagcaccgc ttttaccaaa ttttcgagta aagtaaaaat
     9901 gattttataa cctgcaagtt caacatcaag cacttccgaa gaacagtaaa ttttctcaaa
     9961 agccgttttt gaacagtttt ggtaagcttt cagcgacacg ggaggcagca gttttattaa
    10021 tgtggaactg aaactccctt ccagaatttg aggttcattt tccagaaaaa cagaagaaca
    10081 gttgtcaatg agctgtccaa taaccgaaga acgcaaatag gcaatttgtt cgttgacatc
    10141 atcaaccata gcaatagttt gcagttttct ttcgagtttc ttcccttcaa aataggaaag
    10201 gaaaagagct tttgtttcgt cggtggaaag aattttcagt ttgtgcgcat cttcaatatc
    10261 cattatttgg tagcaaatgt cgtcagcagc ttctaccaga taaaccaacg gataacgaac
    10321 atatttgtcc ggttgtgaaa acgaatgagg aataccgagt tcgtgagcaa ttttttcata
    10381 atcggatttt tcagaatgaa aaaaaccaaa tttttgtcgt tgcggatccg aaaaaactga
    10441 agcataggga tattttacta tggaagccag tgtgctgtat gtgagaacga aaccacccgg
    10501 acgtctgcct ctgaattgat gggtaagcac gcggaaagtg ttggcattgc cttcaaaact
    10561 tattaagtcc gaccattctt cgggcaacat tttctcctgc aaaatttgtc cttctccttc
    10621 cgagaaatag gttgctatgg ctttttcgcc ggaatgtccg aaaggcggat ttcccatgtc
    10681 gtgagccaga caagctgccg aaacaatgct cccgacttca tcaataaaag catcgggtaa
    10741 attatttttc tcttttattt gacgtgccac attgatcccc agcgagcgcc ctacactgga
    10801 aacttccaag ctgtgcgtaa gccgattatg aacgaaaacg cttccgggaa gtggaaaaac
    10861 ctgtgttttg ttctgtaaac gacgaaatgg aggagaaaaa atcaatcggt cgtaatcgcg
    10921 ctgaaattcg ctgcggttat cctgacgctt ttcatgatag gcttccatcc cgagtctttt
    10981 ggtggtaatc aattgcttcc agttcatatt cacgaattta ttattgataa aacaaatttc
    11041 ggttgaaaac gttagcgcct agtcccaatt gaagttgaaa tttttcaatt cttctttcaa
    11101 tctgtcgctt tggttgtcgg taacgcgatt caaccagcgc caaaagtccg tactgtgatt
    11161 aagttccctg gtatgacaaa gttcatgtaa aataacatag tcgattaagt gcgccggtaa
    11221 agccatcaga taaagagaca aatttatgtt cccctttgcg gagcaacttc cccagcgggt
    11281 acgacttgat tgaatcttga cagtggaaaa cctgaaacca aatttgtctg ccaacagtct
    11341 ggtgcgttgg ggtaaaactc ttttcgcttc taacctgaga taacgagcaa taatcctgtg
    11401 taaataattt tgcatttgtt cttccctgca atcaacttcc gaaggaattt caatggagag
    11461 aacctgattt ttcagacaac aatagatatc ttctcgatgc gtgggtttga tatcagtaac
    11521 aaaagtcagc gccctgaaag gggattgttc atcaataatt tgtttgtttt tgcgtatctt
    11581 ttcctttact tgagctcgtt tttttaaaat tttttcacga accgagtcga taaatctcat
    11641 tgcatctttt tcgcttgccc gcatgggcag ggtaactttc aatttatctt ctaaaatttg
    11701 aatgtttatg cgcgtagccc gttgacttct gttgaaataa atatcaccca gttcggggtc
    11761 ggaaattttt ctttgcgaag aagaagtatt catttgtgtt gcgattgaaa gcttattttc
    11821 gatatcgaaa tcggtattga gcagccaaaa caggttctct tttcaattag aaaaaacaaa
    11881 gacaaatgta atcaatcacg ccaaaattga aaaaatcatt tttggttgtc ttgtaaattt
    11941 gataagcaat aacttatgaa gaaagtacta tcaattattt tattagcctt tccatcgctt
    12001 caatggtgtt ttctctgctg ccaaatgccg agaaacggat atatccttcc ccactgcgac
    12061 caaaacctgc tccgggagtt gtaacgatat ttttttgttt cagcagaaag tcaaaatatt
    12121 cccaactttt catattgtcg ggtgccttta cccaaacata gggagcattg acaccgccga
    12181 aaacctttaa acccaatttg gcaacgcctt cacgaatgat gcgggcgttt cccagataat
    12241 attcaataag ttgtttcacc tgtttcctgc cttcgggcgt aaaagtagct tcagcgcccc
    12301 tttgtacaat gtaggaagtg ccgttaaatt tggtagattg gcgtcggagc cacaatttgt
    12361 tcaaagcaac cgcttctccc tgatgcgtat aaccaaccaa ctctttggga acaaccgtgt
    12421 aaccacaacg cgttccggta aatccggcag ttttagaaaa acttcgaaat tcgatggcaa
    12481 cttctttggc cccttctatt tcataaatgc tgtgtggaac attttcttca gtaataaaag
    12541 cttcataagc tgcatcaaac aatatcaccg aacgattggc ttttgcatac tccacccaaa
    12601 cctgaagttg ctgtttgcta agtgtggtgc cggtcggatt attggggtag cagagataaa
    12661 tcatatctac ttttttatcc ggaagctgag gaacaaagtt gttagcttcg ttgcagggaa
    12721 gataggtcag tccgttccat tcgttaaatt cgtttggttc gccggctctt ccggccatag
    12781 cgttggtgtc cacatatacg ggataactcg gatcggtaat ggccacacaa ttgtcgatac
    12841 cgaaaatatc gccaatattg cccgtatcgc tttttgctcc atcgctgaca aaaacctcat
    12901 caacatcgag ttttattcct cttgcccgaa aatcattttc cacaatggcc tgtcttaaaa
    12961 attcataacc ctgttccggc ccataacctc tgaaagtttc cgcatgacgt tgttcttccg
    13021 cagctttgat catagcatct accgaagcgt cgggcaaagg aagtgtaaca tctccaattc
    13081 ccatacggat aattttagct gaaggatttt ctgccttgtg ttgttccacc cttttggcaa
    13141 tttcggaaaa taaataggtt ggaggaattt ttataaaatt ttcgttgata taagccattt
    13201 taatgattcg attaatgatg ataaaaaata cgttttattt tgaaaatcaa tatgttttgt
    13261 agaaaaaaat gaaaacttag gaaatttcgc cttcaaaaac tgtttcagcc ggcccggtca
    13321 aataaacatg ttcatcgttt tcgttccatt ctatttccaa atttccacct aaaagttcca
    13381 cagtagcctt acgcgacaca agcccgttta gcacaccggc cacaaccgat gcacaggcac
    13441 ctgtaccaca agccattgtt tcacccgatc ctctttccca gacacgcatt tctatgcggc
    13501 ttttgtcgat cacctcgata aattccacat tggtacgacg aggaaaaatg ggagcattct
    13561 caattttttt tcccatcttt tcgatatcca tattggcaat attctccgtg aatattacag
    13621 tatgtggatt gcccattgaa acacaagtaa tagcgtaatt gatgccgtca aaggaaacag
    13681 ggaaattaac aatctgtttt cccggaagcg tagtaggaat tttttcagct tccaacaccg
    13741 gttttcccat atcgactgta acggaaacaa cattgtttct ctcgtcaaga aaaagttgca
    13801 gggttttgat accagccaac gtttcaaggg tcaattcggt tttgtctgtc ataccttttt
    13861 catagacata tttacctatg cagcgcgcgg catttccaca catttccgct tccgagccat
    13921 cggcattgaa catttgcatt ctaaaatcgg cttttgaaga aggaagaata attaccaatc
    13981 catcggatcc aactcctttg tgtctgtcgc tcaagcgtat ggataattcg ccgggatttt
    14041 caagctgttc ttcaaatccg ttgatataaa tgtaatcgtt cccgattccg tgcatttttg
    14101 taaatttcat gatgaaaata atttatgtac ttttttcgat gacaaagata aaaaattaaa
    14161 tcaggacagg agaaaattat aattgcgatt ttcaaaacaa gctgtgttta cttataaatt
    14221 atcagaaaac agcttccttt ttgagagaat tcaaacaaaa ttcaaattta atggctgtga
    14281 ttgaatattt tgaactttca tcacctatcg gccaataact ttttcaatgt ttctgcaaat
    14341 tcatcaattt cttcgggctt tgtatcaaaa gagcacatcc agcgcaattc attggtttga
    14401 aaatcccaag tgtaaaatcg aaatttttcc cgaagaggct caatcacttt ctcgggaatt
    14461 atggcaaaaa cggcattagc ctgcaccggt aaagttattt gaacttcagg tatagcgcgc
    14521 attttttctg ccagtaaact tgccatttta ttggaatgtg ttgccatttt cagccacaaa
    14581 tcgttcttga gcaaagccat aaactgtgct gctataaaac gattttttga atacaattga
    14641 agagattgtt tctgataaaa acgggcattg gcattcagtt catcgttaaa aatcaaaatt
    14701 gcctctccca tcatcattcc atttttcgtg ccgccaaaac tcataatatc caccccgcaa
    14761 tcaacggtaa cttcctttaa actttttccc agcgctgcca ctgcgttgga aattcttgcg
    14821 ccatctacat gaacaagcat gttgttttta tgtgcaaaat cacataatgc tttcagttct
    14881 tcggtgtcat atagcgttcc cacttctgtg gtttggctaa tggaaatcac ttttggttgt
    14941 gcgctgtgca cattgccttt gtgtaccaaa accggggaaa tcagttcagg agtaagtttt
    15001 ccgtccggcg tatgcaccgg cataattgaa catccgatat tttgaaaaac tgcaccgcat
    15061 tcgtcagtca aaatatgagc cgtttgggca cacacaatcg attgaaaggg caatgtacag
    15121 cactttaaac aaacaatatt ggcacctgta ccattgaaaa caaacaaaac ttccacattt
    15181 cccaacttct gctgaaaaag tttttttgcc cgttgggtgt aagtgtcatc gccataagaa
    15241 atttcatgat cggcattggc ttctacaatg gctttaagga tatcgggatg aatgcccgaa
    15301 taattgtcgc ttgcaaaaca tttcatataa tttctttttt acaaaagttt aatgataatc
    15361 cgcaaagtat cctaaattaa aaggataaat tatggtatat ttttcagaaa atagttataa
    15421 aaatttgtat aat
//
LOCUS       DAOSIB010000056        15014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_4109_length_15014_cov_12.254696, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000056 DAOSIB010000000
VERSION     DAOSIB010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15014
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4109_length_15014_cov_12.254696"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..753)
                     /locus_tag="PLD44_06150"
     CDS             complement(175..753)
                     /locus_tag="PLD44_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOF98950.1"
                     /translation="MDKSLKGTRTEQNLLKAFAGESQARNRYEFFASVAKKEGYEQIA
                     AIFMETASQEKEHAKRFFKFLEGGMVEITASYPAGIIGTTKQNLKAAAEGEYEEWSSL
                     YPTFAEVAKEEGFPEISVAFKMVAKVEVEHERRYLKLLQNISEDKVFKKDGKVLWQCR
                     NCGYIYESEEALEVCPVCLHPKAFMQLKEDNY"
     gene            complement(789..1469)
                     /locus_tag="PLD44_06155"
     CDS             complement(789..1469)
                     /locus_tag="PLD44_06155"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOF98951.1"
                     /translation="MIQLEALYKISYGLYIVTSGDRNQGNGFVSNTVFQVTSQPQRFA
                     VCCNKDNYSASFIQQFKAFAISILAQDASAELIGKFGYKSGREMNKMTDVKVIFKETN
                     VPIVLDGSLAFFECKLVDVFDVGTHWIFIGELVSAEIVDDTGEPLTYAYYREVKKGFS
                     PKNAPTYIDQSKIETKTENAMSKKFKCPICGYIYDEAEEKVKFSDLPDDWVCPVCGSE
                     KSEFIEIS"
     gene            complement(1581..3869)
                     /locus_tag="PLD44_06160"
     CDS             complement(1581..3869)
                     /locus_tag="PLD44_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOF98952.1"
                     /translation="MKKINLLFIVLSLVAVVNAQNKPATPQLGKDSLKEVIKAMTLED
                     KIYMVMGLGEDTWKNPATGPKTVLLAGAAGRTWDIPRLGITPAILTDGPAGLRIEPKQ
                     EGFSHPFYCTAFPTATVLASTWNTPLVEEVGKAMGNEVLEYGSDVLLAPALNIQRNPL
                     CGRNFEYYSEDPLIAGKMAAAMVVGLQSNGVGTSVKHFAANNIETKRTTINAIVSQRA
                     LREIYLRGFEIAVKEGNPWTVMSSYNRLNGFYTSQNPDLLTTILRKEWGFKGMVMSDW
                     WGGDDAVAQMKAGNDLLEPGATQREVLREALKNKTLSEKTLDRNLMRILDFIQKTPHF
                     KGYQPSYHPDLEAHAVIARKAAVEGMVLLKNENSTLPFKKVKKIALFGKTSYQFMTGG
                     TGSGEVNYDHAAALDEAFGTAKFQLSKTLTDRYRQFIDSVRQHEKKENQKFAVDFAPE
                     LNLTKDFIQSQVKNSDIAVITIGRQGGEGWDRPESTFSLSEVEKNLIKNVCTSFHAAK
                     KKVVVVLNIGGVIETADWRDWPDAVLLAWQPGQQGAHAVVDLLKGTENPSGKLAVSFP
                     MKYADVPSAATFPGEPKDNPVNAFYTEGIYVGYRYYETFKVPTAYPFGYGLSYTSFKY
                     SDLKLSNTLFDNEISVSVKITNIGKTAGKEVVQLYREAPKQAIDKPVKELKAFAKTQL
                     LKPNESEVITFKLDARSLASFWSGVSAWIADEGKYKIHVGASSEDIRATGDFSLPKKI
                     MVEKTHDVLYPNLLLHEISPEK"
     gene            4328..6967
                     /locus_tag="PLD44_06165"
     CDS             4328..6967
                     /locus_tag="PLD44_06165"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOF98953.1"
                     /translation="MELPSKYNPEGIESKWYQYWLKNGFFHSTPDQRQPYTIVIPPPN
                     VTGVLHMGHMLNNTIQDILVRRARMQGKNACWVPGTDHASIATEAKVVEKLAAEGIKK
                     TDLSREEFLNHAWEWTRKHGGIILEQLKKLGASCDWDRTAFTMDEIRSQSVIKVFVDL
                     YEKGKIYRGIRMINWDPKAKTALSDEEVIFKEQEGKLYYLKYKLCPTTENDNSDEYLI
                     VATTRPETIMGDTAVCIHPADPRYQHLKGRKCIVPLVNREIPIIEDEYVDMEFGTGCL
                     KITPAHDVNDYMLGEKHHLPSIDIFNDDGTLNENGGSYAGMDRFEVRKKIVKDLEVAH
                     LLEKTENYINKVGFSERTDVPIEPKLSMQWFLKMEELAKPALEAVMTDKIKLYPPKYK
                     NTYRHWMENVKDWCISRQLWWGHRIPAYYFSNGGWVVAETKEEAYQKALKIANEPFPI
                     EDLRQDEDCLDTWFSSWLWPISVFNGILEPDNEEINYYYPTDDLVTAPEILFFWVARM
                     IIAGYEYRGEKPFKNVYLTGIVRDKLGRKMSKSLGNSPDPLLLIEQYGADAVRMGMLL
                     TSPAGNDLLFDESLCEQGRNFNNKIWNAFRLVKGWQTAEIEQPEASRLAVEWFRHQLN
                     KSLYEMEDLFGKYRLSEALMTVYKLFWDEFASWYLEIVKPGYGQPVDLKTYNATLEFF
                     DVLLKILHPFMPFITEELWQQLSKRKDGESIMMQPHPQPENFDENIIKEFETVKEIVA
                     GIRSVRLQKNIPNKEVLKLLVNGKDNTNFYSVVKKLGNIKSIEKTAEKAPGSVSYMVG
                     TTEYIIPTGNKVDIASEIKKLEEELAYLEGFLASVMKKLNNEKFVANAQPQVVENERK
                     KKADAESKIASLRESIEALRKIS"
     gene            7617..8489
                     /locus_tag="PLD44_06170"
     CDS             7617..8489
                     /locus_tag="PLD44_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOF98954.1"
                     /translation="MSFNIVVLAKQVPDTRNVGKDAMKADGTVNRAALPAIFNPEDLN
                     ALEQALRIKEKVPGTVVHLLTMGPVRAAEIIRESLYRGADDGFLLSGREFAGSDTLAT
                     SYALSCAIRKLGKIDLILCGRQAIDGDTAQVGPQVAEKLGLPQITYAEDIIEITKKNI
                     TVKRRLERGVEIVKGPLPILVTVNGTAAPCRPRNAKLTLKYKHAATPSEKQESGSDYT
                     DLYDAHPYLNITEWGANDIEHDPKWLGLSGSPTKVKKIDNIVFTAKESKRLTGSDFEI
                     ESLMKELIANHTIG"
     gene            8579..9598
                     /locus_tag="PLD44_06175"
     CDS             8579..9598
                     /locus_tag="PLD44_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOF98955.1"
                     /translation="MNNLFVYLEIEDGIVKDVSLELLTKGRSLAKQLNCKLEAVAIGY
                     QLENIPKQVFPYGVEVLHLADDKRFVPYTTQPHASVLIHLFKEEKPQIALLGATSLGR
                     DLGPRVSSALFSGLTADCTSLEIGDYEDKKAGKVYKNLLYQIRPAFGGNIVATIVNPD
                     CRPQMATVREGVMKKEIFDPKHTGEIRRLEVSKYVSDQDFVIEVIERHIEKTKIDIKS
                     APIIVSGGYGVGSAENFKLLYELAEVLGGEVGASRAAVDAGFAEHERQIGQTGTTVRP
                     KLYIACGISGQIQHIAGMQESAMIIAINSDPNAPINKIADYIITGRVEDVLPKMIKYY
                     KKNTK"
     gene            9712..11421
                     /locus_tag="PLD44_06180"
     CDS             9712..11421
                     /locus_tag="PLD44_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOF98956.1"
                     /translation="MMNFYNDNPALKFQLSHPLMQKIVTLKERNFADKDKYDYASRDF
                     EDAIDNYEKILEIIGEICGEVIAPNAEEVDHTGPQVVNNRVVYAPGTQQNHEVLTQAS
                     LYGIGLPRQYGGLNFSMVPYVMAAEMVSRADAGFANIWGLQDCAETINEFADEDLKRE
                     FLPRVCQGQTCSMDLTEPDAGSDLQAVQLKATFNEKDGIWYLNGVKRFITNGDAHIKL
                     VLARSEEGTTDGRGLSLFLVDNKHDDTVKVRRIENKLGIKGSPTCELVFSNTPAKLIG
                     SRRLGLIKYVMSLMNSARLGVGAQSVGLSASAYNEALKYAKERQQFGKAIIEFPAVFE
                     MISLIKAKLDASRALLYETTRFVDMYKAYEAIEKERRLEPEEKAEYKEFQRLADAFTP
                     LLKMFASEYANQNAFDCVQIHGGSGFMKDYACERLYRDARILTIYEGTSQLQVVAAIR
                     HVTTGTYLQQIREYEKLPINPEFHSFRKRLMIMTNLYENSVSRVIETKNQEYIDFQAR
                     RLVEMAGHIIMGYLLILDATRNDMFRKSAEVYINYGEVETRKHATFIKTFDMENIDIY
                     KVV"
     gene            11820..14666
                     /locus_tag="PLD44_06185"
     CDS             11820..14666
                     /locus_tag="PLD44_06185"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HOF98957.1"
                     /translation="MKKIHLLFIIFCACAANAQEFIPLVTQFSKNDYRAANQNWAVAQ
                     GLDGILYFGNNEGLLEFDGSLWKLHKIPGNGLVRSVMIDKKNRIYIGAFEEFGYFERD
                     RFGQLNYTSLSALLKNYKMQNDEVWNIFDDQGTIFFQSFTSYFTFKNGEVKGFRTDFS
                     FLFFNLYQNTLYTHTFQKDFCYFDRQQNTFVPVPNNIMKSPVIKILPFDDKHALMVTI
                     SDGLYLYDGDKFTRFASEVDDVLKKAQINRAVISLNGLIVIGTILNGAVAIDKSGRKI
                     WAISSSNVLQNNTVLGITFDRDNNIWLALDKGIAFIHQNSSFRCIQSFYPAIGAIYSV
                     SYDPPHIYIATNQGLYRALLEHHSIRNLSIDPKIKGQVWDVSSFDGQQFCGNNEETFE
                     VTPTRSIPVSPVKGGMCFAHGNIHGVEVLVQGTYTQLCIYKKENGKWKFSHAVEDFLH
                     PIRYIEIDYTGTIWASHLHRGLFAIQLTPDLRKIEHINIYKSLDQQHLNTINVFSVNN
                     RVVFTDYNLFYTFDDIRKKIVPFDELNKALGNFSNSYRICYFKGNLYWFIRPGEAALV
                     EIKPGQIKLVDMVQYADFLHQTVDDYQNIIPISENECIFTLENGLALYNYTQKLIPQY
                     SIQLKLKSIQISDAANKKLNFLPLEATSLPTIPYSRNNVVFSVFYPEYAQRNNVMYRF
                     KLEGLDVVWRDPSSSPSIEYKYLPYRKYVFKAEAISESGQILSETNFAFEVQPPFYLS
                     RPAKIIYTLAVLFLLWGIYWYVQRIIHRKKEKVRIEQEDIRRKEIEKREQQIITLEKE
                     KLESELTLKSKELATSTITLIRKNEVLATIKEEIMQQKAALGSQYPNKYYEKIIRLLD
                     ENLSSEDDWAIFQANFDRIHENFFRHLHERYPELTPNDLRFCAYLRLNLSSKDIAHLM
                     NISLKGVEMGRARIRKKIGLPSTKSLTEFMIEFK"
BASE COUNT         4793 a         2918 c         2897 g         4406 t
ORIGIN      
        1 ggaacaacaa gaagccgaaa aagagaaaca tgtagcgtat gatgaatgca tactttccga
       61 atcgggttac tatgattatg aacttgatga agatgaaaac gactaaatcg agtagggcgg
      121 gggagtaatc cccctacctg ctcaattaga gttcaatttt ttcgagaaag tttttcaata
      181 attgtcttct ttcagttgca tgaaggcttt ggggtgcaag caaaccggac aaacttccag
      241 cgcttcttcc gattcgtaaa tgtagccaca atttctgcat tgccacagca ctttgccgtc
      301 ttttttgaaa accttgtctt cggaaatatt ctgtaaaagt ttcagataac gacgttcgtg
      361 ttccacttct actttagcca ccattttgaa tgctacggaa atttcaggaa atccttcttc
      421 tttggcaacc tcagcaaatg ttggatataa acttgaccat tcttcatact ctccttcagc
      481 tgcagctttc agattttgtt tggttgttcc gataattcct gcaggatagg aagcggtgat
      541 ttctaccatg ccaccttcca gaaatttgaa gaatcttttg gcgtgttcct tttcctgact
      601 ggcagtttcc ataaagatgg cagcaatttg ttcgtaacct tccttcttgg caacgctggc
      661 aaaaaattcg taccggttac gtgcttgcga ttcgccggca aaggctttca gcaagttttg
      721 ttctgttctt gtccctttta acgatttatc cataattttg ttttttaatg agttactgaa
      781 tgatttattt aagaaatctc tataaactcc gatttttctg aaccacaaac cgggcaaacc
      841 caatcatcgg ggagatcgga aaattttact ttttcttcgg cctcatcgta aatgtagccg
      901 caaattggac atttgaattt tttcgacata gcattttcag ttttagtttc tatttttgat
      961 tgatctatat acgttggagc atttttgggt gaaaaccctt tttttacctc tcgataatac
     1021 gcataagtca aaggttcacc ggtatcgtcg acaatttcgg cactaaccag ttcgccaata
     1081 aaaatccaat gtgttcccac atcaaaaaca tcaaccagct tacattcaaa aaaagcaagg
     1141 ctgccatcga gtacaatagg gacattggtt tccttaaaaa taacttttac gtccgtcatt
     1201 ttattcatct cccttccgct tttatatcca aacttgccaa taagttcggc ggaagcatcc
     1261 tgtgccagta tggaaatggc aaaagccttg aactgctgaa taaacgaagc ggaataatta
     1321 tccttgttac agcaaacggc aaaacgctga ggctgagaag taacttgaaa taccgtattt
     1381 gaaacaaaac catttccctg gtttctatct ccagaagtta caatatataa cccataagat
     1441 attttataaa gtgcttctaa ctgtatcaca gagtaagaat taaaaaagat atttgaaaaa
     1501 acaactaaaa aaatctatga caaaaatagt tttaaatttc aaattataac gatattgaaa
     1561 caaaaataaa ttttctcatc tcatttttcc ggagatattt catgcagtaa taaattagga
     1621 taaagaacat catgtgtttt ttccaccatt attttttttg gcaaagaaaa atcaccggta
     1681 gcgcgaatat cttccgatga ggctcctaca tgtattttgt atttgccttc gtcagcaatc
     1741 caagcactga caccactcca gaaagaagcc aaagaacggg catccagctt aaaagttatc
     1801 acttcactct cgttgggttt tagcaattga gttttggcaa aagctttcag ttcctttaca
     1861 ggtttgtcga tggcttgttt gggagcttcc cggtaaagtt gaacaacttc tttgccggct
     1921 gtttttccga tattggtaat ttttaccgaa acgcttattt cgttatcaaa caaagtattg
     1981 ctcaacttca aatcggaata tttgaaactg gtataggaaa gtccataacc aaaaggataa
     2041 gctgtcggca ctttgaaagt ttcataatac cgataaccga catatatacc ttctgtataa
     2101 aaagcattga cggggttatc tttgggttcg ccgggaaaag ttgcagccga aggaacatca
     2161 gcatatttca tcggaaagct gacagccagt ttgccggaag gattttctgt acctttcaac
     2221 aaatcaacca ccgcatgagc accttgttgc ccgggttgcc atgcaagcaa aacagcatca
     2281 ggccaatctc tccaatcggc agtttcgata acgcctccaa tattgagcac cacaacaact
     2341 ttctttttgg cagcatggaa agaggtacag acatttttta tcagattttt ctcaacttcg
     2401 ctcaatgaaa aagtcgattc gggacgatcc caaccttcac cgccctgacg tccaatagta
     2461 ataaccgcaa tatcggaatt ttttacctgc gattgaataa agtctttggt taaatttaat
     2521 tccggtgcaa aatcaacagc aaatttttga ttttctttct tctcatgttg tcttaccgaa
     2581 tcgataaatt gtctgtaccg atcggtcaaa gttttcgaca attgaaattt tgcagtacca
     2641 aaagcctcat ccaaagctgc agcatgatca taattcactt cgccacttcc ggttccgcca
     2701 gtcataaatt gataggaagt ttttccaaac aaagctattt tttttacttt tttgaatggc
     2761 aaagtggaat tttcgttttt caataaaacc ataccttcaa ctgctgcctt gcgagcaata
     2821 acggcatgag cttccaaatc gggatgatac gacggttgat aacctttgaa atgaggagtt
     2881 ttctgaatga aatccagaat tcgcatcaaa tttctgtcaa gcgttttttc gcttaaagtt
     2941 ttgtttttca gtgcttccct gagtacttct cgctgtgtag ctccaggttc caacaagtcg
     3001 tttcctgctt tcatttgagc taccgcgtca tcgccacccc accaatcgga cattaccatt
     3061 cctttgaatc cccattcttt acgtaaaatg gtagtcagca aatcgggatt ttgagaagta
     3121 taaaatccat tcaagcggtt gtaggaagac attaccgtcc aaggatttcc ttctttaaca
     3181 gcaatttcaa aacctctcag ataaatttct cgcagtgcac gctgactgac aattgcatta
     3241 attgtggtgc gtttggtttc tatattgttg gcagcaaaat gcttaacgga agtgccgacg
     3301 ccattggatt gcaatccaac aaccattgca gcggccattt taccggcaat gagcggatct
     3361 tcggaataat actcaaaatt tcgcccacaa agcggatttc gctgaatgtt caatgccgga
     3421 gccaataata catccgaacc atactccaaa acctcgttac ccatagcttt tcctacttct
     3481 tctaccagtg gagtattcca agtagaagcc agcactgttg ccgttgggaa tgccgtacaa
     3541 taaaaaggat gagaaaagcc ttcttgcttt ggttcaattc gtaaaccggc agggccatca
     3601 gtgagaatag caggtgtaat gcccaatcga ggaatgtccc atgtacgacc ggcagcaccg
     3661 gcaagcaata cggttttagg ccctgtagcg ggatttttcc atgtatcttc tcccaaaccc
     3721 atgaccatat aaattttgtc ttccaaagtc atggctttga tgacttcttt taagctgtct
     3781 ttgcctaatt gcggcgttgc aggcttattt tgagcattta caactgcaac caaagacaac
     3841 acgataaaaa gcaaattgat ttttttcata ccattatgtt ttttttgtta ttgagaaaaa
     3901 agcatatccc tattttatta tccttttaaa tgagaaagga atggattttc tgaaaaaata
     3961 ccgaatatat aaaatcgttc ctgttgaaag gttaaaaaac cgtataaaat aactgttcga
     4021 taaaaatttt accgtacttt ttacgtcttt ctgccaaatc ctatatctac cgttatcatt
     4081 tcaaaataca actgacatta aaattgctca tctcaaataa attagaacgg gcctttgttc
     4141 gccttaaggt tcagcttctc gattcaaatt ttttcggtct aaaaattcgt cagttggtgc
     4201 aaattttgat tatctttgca tttcaaaaaa ataagacaaa gactttcact atcaatttga
     4261 tagacaaata attaaatttt cacaacttat cgagcatcat cactttgaaa ttgtaaaagt
     4321 ataaaaaatg gaactaccga gcaaatacaa tcccgaagga attgaatcaa aatggtatca
     4381 atattggttg aaaaatggtt tttttcattc aacacccgat caacgtcaac cttacacaat
     4441 tgtaatcccg cctcccaatg taacaggagt acttcacatg ggacacatgc tcaacaatac
     4501 cattcaggat attttggtac gtcgggctcg catgcaggga aaaaatgcct gttgggtgcc
     4561 gggaactgat catgcatcca tagccaccga agctaaagtt gtggaaaagc ttgcagccga
     4621 aggaattaaa aaaaccgacc tttcacgtga agagtttttg aatcatgctt gggaatggac
     4681 gcggaaacat ggcggcatta ttctggaaca gctaaaaaaa ctgggagctt catgcgattg
     4741 ggatcgtaca gcttttacaa tggacgaaat ccgttcacaa agtgtaatca aagtttttgt
     4801 ggatttatac gaaaagggaa agatttatcg cggcattaga atgataaatt gggacccgaa
     4861 agcaaaaacg gctttgtcgg acgaagaagt aatttttaaa gaacaggaag gaaaacttta
     4921 ttacttgaaa tataaacttt gtccgactac cgaaaacgac aacagtgatg aatatctgat
     4981 tgttgcaacc acacgccctg aaaccatcat gggagatacg gcagtttgca tccatcctgc
     5041 cgatccccgc taccaacatc tgaaaggtag aaaatgtatt gttccattgg taaaccggga
     5101 aattccaatt attgaagacg aatatgttga catggaattt ggaactggat gtctcaaaat
     5161 tacacccgct cacgacgtaa acgactacat gctgggcgaa aaacatcact tgccttccat
     5221 tgatattttc aatgatgatg gcacactcaa tgaaaatggt ggtagctacg ccggcatgga
     5281 tcgttttgaa gtccgcaaaa aaattgtaaa agaccttgaa gtagcccatt tactcgaaaa
     5341 aacggaaaac tacatcaata aagttggttt ttctgaacgt acggatgttc ccatagaacc
     5401 aaaattatcc atgcaatggt tcctgaaaat ggaagaactt gccaaacctg cactggaagc
     5461 cgtaatgact gataaaataa aattgtatcc tccaaaatac aaaaatactt atcgccattg
     5521 gatggaaaac gtaaaagact ggtgtatttc ccgacaactt tggtgggggc atcgcatccc
     5581 tgcctattac ttttcgaatg gtggttgggt agttgctgaa acaaaagaag aagcttatca
     5641 aaaagcactc aaaatagcga acgaaccatt tccgattgaa gatttaagac aggacgaaga
     5701 ctgtctggat acatggtttt cttcgtggtt gtggccaata tcggttttca atggtattct
     5761 cgaacccgac aatgaagaaa ttaactatta ttatcctact gatgatttgg ttacggctcc
     5821 ggaaatttta tttttctggg ttgcccgcat gatcatagcc ggatatgaat acagaggcga
     5881 aaaacctttt aaaaacgttt atctgaccgg tattgtaaga gacaaattgg gaaggaaaat
     5941 gtccaaatcg ctgggaaatt cacccgatcc gctgcttttg atagaacaat acggagctga
     6001 tgccgtccgt atgggaatgt tgttgacttc cccggccgga aacgatttac tttttgacga
     6061 aagtttgtgc gaacaaggaa gaaatttcaa caacaaaatt tggaatgcat tcagactggt
     6121 aaaaggctgg caaaccgccg aaattgaaca acctgaagcc tcacggcttg cagtggaatg
     6181 gttccggcat caactcaaca aatcgttgta tgaaatggaa gatttgttcg gcaaatatcg
     6241 tttgtcggaa gcattaatga cggtttacaa gcttttctgg gacgaatttg cttcatggta
     6301 tttggaaatt gtaaaaccgg gatacgggca acctgttgac ctcaaaactt acaatgctac
     6361 gttggaattt tttgatgttt tattaaaaat attgcatccc ttcatgcctt ttatcacgga
     6421 agagttgtgg caacagctgt ccaaaagaaa ggatggcgaa agtattatga tgcagcccca
     6481 tcctcaacct gaaaactttg atgagaatat aataaaggaa tttgaaaccg ttaaggaaat
     6541 tgtggccgga attcgcagtg tacgtttaca aaaaaatatc cccaacaaag aagttttaaa
     6601 gttactagtt aacggaaaag acaatacaaa tttttattcg gtcgtaaaaa agctcgggaa
     6661 tatcaaatcg atagaaaaaa cagcggaaaa agcacccggc tctgtttctt acatggttgg
     6721 aacgaccgaa tacattatcc cgacaggcaa caaagtagat atagcttccg aaataaagaa
     6781 gttggaagaa gaattggctt atctcgaagg ctttctggct tcggtgatga aaaaactgaa
     6841 caacgaaaag tttgttgcca atgctcaacc tcaagttgtt gagaatgagc gcaagaaaaa
     6901 agccgatgct gaaagcaaaa ttgcttcttt gagggaatca attgaggctt tacgaaaaat
     6961 ttcttgatca gactttgtca ccattcaatt tcagctatta aaaaatttat tagcaaattt
     7021 tgagatggat tccacatcga ttttataaat tttgagatat ttcttatttt catcgaagat
     7081 gcgcttcgac gttttttaaa attattggtt caagaggtat aaaaaagaag ttcgactttt
     7141 agaaaactta ttacttgttt atgattcgat agaacggtaa tatttttaac atttcaagcc
     7201 aaagacaatt taagtgtctt taaattaata gattaagttc ataaaatata attatctgaa
     7261 aattgttaaa agtcgaattg ctccaaaaat aggttgtaaa acataaattt tgacttgcag
     7321 gttttatttt ttaatgcgta ttttgcaagc gttaactaaa caacacaatc ttttttatac
     7381 ctttaaaact aatacctatt aaaaaacgcg aggccttgtg aaatgtctcg cgctttttat
     7441 tgaataaagg cctcaaattt tatattagag acaacaaatt gcaacttcaa taaaaacgac
     7501 taattttgca aatcaaaaaa aatcggatac aaaattcttc agctttgctg taacaaaaaa
     7561 cttttcgcag ttgaagaaga aagacttaat attaattaca ataatttaat gataaaatga
     7621 gtttcaatat cgtagtttta gccaaacagg tgcctgatac ccgaaatgtg ggaaaagatg
     7681 ccatgaaagc cgatggaaca gtgaaccgtg ctgcgctgcc ggctattttc aatccggaag
     7741 atttgaatgc tcttgagcag gcacttcgca tcaaagaaaa ggttcccggc acagttgttc
     7801 atttgctgac aatgggtccg gttcgtgctg ctgaaatcat tcgtgaaagt ctctatcgcg
     7861 gggctgacga cggatttctt ttaagtggaa gagaatttgc cggctctgat acgttggcaa
     7921 cttcctatgc tttatcctgc gccattcgca agttgggaaa aatcgatctg atcctttgcg
     7981 gccgtcaagc catagatgga gatacggcgc aagtggggcc tcaggttgcc gaaaaactgg
     8041 gcttaccaca aatcacatac gccgaagaca ttattgaaat taccaagaaa aatatcaccg
     8101 ttaaacgccg actggaaaga ggtgtggaaa ttgtcaaagg gccactgccc atattggtta
     8161 ccgttaacgg aacagcagct ccatgccgcc caagaaacgc caagttaaca cttaaataca
     8221 aacatgctgc aaccccatcc gaaaaacagg aatcgggttc tgattatacc gatctgtacg
     8281 atgcacatcc ctatttgaac attaccgaat ggggtgccaa tgacattgaa cacgatccaa
     8341 agtggcttgg attgagcggt tcacctacta aagttaagaa aatcgacaat atcgttttca
     8401 cggcaaaaga aagtaaacga ctcacaggca gcgactttga aattgaaagt ttaatgaaag
     8461 aactcattgc taaccatact atcggttaat tttgagtatc tctaaaaaat ttttctaatc
     8521 taatttattg gcaaaaaaaa tagaaaattt cctgtcagga aaaaagaatt aaatatatat
     8581 gaacaactta tttgtatatc ttgaaataga agatggaata gtgaaagacg taagcctcga
     8641 attgcttaca aagggacgtt cgttggcaaa acagctcaat tgcaagctgg aagctgtagc
     8701 aattggttat cagcttgaaa atatacctaa acaggtattt ccttatggag tagaggtatt
     8761 gcatttggct gacgataaaa gatttgttcc ttacaccaca caacctcatg catcggtgct
     8821 aattcatctt tttaaggaag aaaaaccaca aattgcactt cttggtgcta cttcacttgg
     8881 gcgcgatttg ggtccaagag tatcttctgc tcttttcagc ggattgaccg ccgactgtac
     8941 ttctctcgaa attggcgact atgaagacaa aaaagccggt aaagtgtata agaatttact
     9001 ttatcagatt cgtccggctt ttggtggcaa tatagtggct actattgtga accccgattg
     9061 caggccacag atggcaaccg tacgcgaagg cgtaatgaaa aaggagattt ttgatccaaa
     9121 acatactggc gaaattcgtc gattggaagt ttcaaaatac gtttcggacc aagactttgt
     9181 aattgaggtg atcgaacgac atatcgaaaa aactaaaatt gatattaagt cggctccaat
     9241 tattgtttcc ggaggatatg gagtaggaag tgccgaaaac ttcaaattgc tttacgagct
     9301 ggcagaagtt ttgggaggag aagttggggc ttctcgtgct gctgttgatg caggttttgc
     9361 tgaacacgaa cgtcaaatag gacaaaccgg aacgaccgta cgtccgaaac tctatattgc
     9421 ctgtggcatt tcgggacaga ttcagcacat tgccggaatg caggaaagtg ccatgattat
     9481 tgccatcaac agtgatccca atgcacctat caataaaatt gccgattaca taatcaccgg
     9541 aagagtggaa gatgtgcttc cgaaaatgat aaaatattat aagaaaaaca caaaataaga
     9601 aacaaactca gcgaatgaaa aaatttcaga aaatcgaatc aataaaattt atttaaacgt
     9661 ttcctgttat tattcgcgct tcaattgttt tcttaattta ataaccgtaa aattatgaac
     9721 ttttataacg ataatcccgc actaaaattc caactttcgc atcccctgat gcagaaaatt
     9781 gttacactta aggaaagaaa ttttgccgac aaggacaaat atgattatgc ttcacgcgat
     9841 tttgaagatg ccattgataa ttatgaaaaa attttggaaa ttatcggcga aatttgtgga
     9901 gaagtgatag ctcccaatgc cgaagaagta gatcatacag ggcctcaggt tgtcaacaac
     9961 cgtgttgttt atgctcccgg gacacaacaa aaccatgaag ttctgacgca agccagcctt
    10021 tacggaattg gattgccacg tcagtatgga ggattaaatt tttcgatggt gccgtacgta
    10081 atggctgctg aaatggtttc gcgtgccgat gccggttttg ccaatatttg gggtttgcag
    10141 gactgcgctg aaactatcaa cgaatttgca gacgaagact taaaacggga atttcttccg
    10201 agagtatgtc agggacaaac ctgttcaatg gatttgaccg aacctgatgc tggttccgat
    10261 ttgcaggctg tgcaactgaa agctactttt aacgaaaagg acggaatttg gtatcttaat
    10321 ggagttaagc gttttattac caatggcgat gctcacatta aactggtgct ggcccgttcc
    10381 gaagaaggga ctaccgatgg tcgtggattg tcgcttttcc ttgtggacaa caaacacgat
    10441 gatactgtaa aagttcgccg tatcgaaaac aaactgggca tcaagggctc tccaacctgc
    10501 gaactggttt ttagcaatac gcctgcaaaa ctcataggtt ccagacgctt gggattgata
    10561 aaatacgtta tgtcgctcat gaacagtgcc cgacttggcg taggagccca atcggtggga
    10621 ctttcggctt cagcatataa tgaagctttg aaatatgcca aagaacgtca gcagtttgga
    10681 aaagccatta ttgaatttcc cgcagttttt gagatgattt cacttataaa ggctaagctc
    10741 gatgcttcac gtgccttgtt gtacgaaact acccgttttg tcgatatgta caaagcgtac
    10801 gaagccattg aaaaagagcg ccgtctcgaa cctgaagaaa aagccgaata caaggaattt
    10861 cagcgtctgg ccgatgcttt tactccactt ttgaaaatgt ttgccagcga atatgctaac
    10921 cagaatgctt tcgactgtgt gcagattcat ggcggttcgg gatttatgaa agactatgcc
    10981 tgcgaacgac tttatcgcga tgcacgtatt ctgaccatat atgaaggaac ttcccaatta
    11041 caggttgtag ctgccatccg ccacgttact accggaactt atcttcagca aatacgtgaa
    11101 tatgaaaaat tgcccatcaa tcccgaattt cattctttca gaaaaagatt gatgattatg
    11161 accaacttgt atgaaaattc ggtgagccgt gttatcgaaa ctaaaaatca ggaatatatc
    11221 gattttcagg ctcgacgact ggtagaaatg gcagggcaca tcatcatggg ttatttgctg
    11281 attctcgacg ctacccgaaa cgatatgttc cgcaaatcgg ctgaagtcta tatcaattat
    11341 ggtgaagttg aaaccagaaa acatgctact ttcatcaaaa ctttcgacat ggaaaacatt
    11401 gatatttaca aagtagtttg agtttatcat tacattttta gcaaacgctc agggttaaaa
    11461 aactttgggc gtttgtattt tgaaatagct ttgacttatt ttaaaatgat taaaaaagtt
    11521 tctatataat tggtaattaa atagttacat tgataaaaag tatttttaaa aattaaaatc
    11581 tgaatttaag aatgagattg ccaaattcag tttatttttt tattaagccg gttatttcta
    11641 aaaaatatcc ttattaactc ctgagtcaac aaatattttc ttatttttga aattcttaat
    11701 ttctgatgtt cctgcaagaa tttaccgatt gattttgtcg aaaaaatcat tttataatct
    11761 tatgaacttt ttgaccagaa ctccggttaa taccttgaaa aattgacatg tacaccttta
    11821 tgaaaaaaat ccatctgctt ttcattattt tctgtgcttg cgcagcaaat gctcaggaat
    11881 ttattccgct cgtaactcaa ttttcaaaaa acgattatcg cgctgccaat caaaattggg
    11941 cggtagcaca aggtttggac ggcatcctgt attttgggaa caacgaagga ctgctcgaat
    12001 ttgacgggtc tctgtggaaa ttgcacaaaa ttcccggaaa tggtctggtg cgttctgtta
    12061 tgatcgacaa aaaaaacaga atttacattg gcgcttttga agaatttggc tattttgaga
    12121 gagaccgttt tggacaatta aattatactt ccctgtctgc cttgctaaaa aattacaaaa
    12181 tgcagaacga tgaagtttgg aatatctttg acgatcaggg caccattttt tttcaatctt
    12241 ttacttcata ttttacattt aaaaacggag aagtaaaagg attcagaacc gatttctcct
    12301 tcttgttctt taacctctat caaaatactt tatacacaca tacttttcag aaagattttt
    12361 gctacttcga ccggcaacaa aatacttttg ttccagttcc caacaatatc atgaaaagtc
    12421 cggtaatcaa aattctgcct tttgacgata aacatgcttt aatggttacg atttccgatg
    12481 gtctttatct ttatgatgga gataagttta cccgctttgc ctctgaagtg gacgatgtac
    12541 tgaaaaaagc acaaatcaac agagctgtca tttcgctaaa cggattgatt gtgatcggta
    12601 ccattttaaa tggagctgtg gctatagaca agtcgggacg aaagatctgg gcaatcagtt
    12661 cttcaaatgt ccttcaaaac aataccgttc tgggaattac tttcgaccgc gacaacaata
    12721 tttggctggc gttggataaa ggaattgctt ttattcatca aaactcatcc tttcgctgca
    12781 ttcaatcctt ttacccggca attggcgcca tttattcggt aagttatgat cctccacata
    12841 tttatattgc aaccaatcag ggattataca gagccttgtt ggaacatcac tccattcgta
    12901 acctttccat cgacccaaaa ataaaaggac aagtttggga tgtaagttcc tttgatggtc
    12961 agcaattttg tggcaacaat gaagaaacct ttgaagttac acccacaagg tccattcctg
    13021 tttcacccgt aaaaggaggc atgtgttttg ctcatggaaa cattcatggt gtggaggtat
    13081 tggttcaggg aacctatacg caactgtgta tttacaaaaa agagaatggg aaatggaaat
    13141 tcagccatgc agtggaagat tttcttcatc ccattcgcta catcgaaata gattacaccg
    13201 gaaccatttg ggcaagtcat ttgcatcgcg ggctttttgc cattcaactt acgcccgact
    13261 tacggaaaat tgaacacata aacatataca aatctcttga tcagcaacat ttaaacacca
    13321 tcaacgtctt ttctgtaaac aacagagtag tatttacaga ctataattta ttttatactt
    13381 tcgacgatat acgtaaaaaa attgtgcctt tcgacgaatt aaataaagcg ctcggaaatt
    13441 tttccaattc ctatcggata tgctatttca aaggaaatct gtattggttc attcgcccgg
    13501 gcgaagctgc actggttgaa atcaaacccg gtcaaatcaa attggtggat atggtccaat
    13561 atgccgattt tcttcatcaa accgttgacg attatcaaaa tatcattcct atctcagaaa
    13621 atgaatgtat tttcacttta gagaatggac tggcacttta taactacaca caaaaactca
    13681 ttccacagta cagcatacag ttaaaactga aaagcattca aatttcggat gcagccaaca
    13741 agaaattgaa ctttttaccg ctcgaagcca ccagtttgcc gactattcct tattccagaa
    13801 acaatgttgt tttttcggtc ttttaccccg aatatgctca aagaaacaat gtgatgtatc
    13861 gttttaaact cgaaggattg gacgtggttt ggagagatcc atcttcatcg cccagtatcg
    13921 aatataaata tttgccctat cggaaatatg tattcaaagc cgaagcaata tccgaaagtg
    13981 gacaaattct ctcagaaaca aattttgcat ttgaagtgca accacccttt tatttgagta
    14041 ggcctgcaaa aattatctac actttggcgg tgctattttt actttgggga atctactggt
    14101 atgttcaacg aattattcac aggaaaaagg aaaaggtcag aatcgaacag gaagatatca
    14161 ggagaaaaga aatagaaaaa agagaacaac aaatcataac actggaaaaa gaaaagctgg
    14221 aatcggaatt gacgctgaag agcaaggaat tggcaacttc gaccataacg ctaatcagaa
    14281 aaaatgaagt gttggccacc ataaaagaag aaatcatgca acaaaaagca gctttaggat
    14341 cacaataccc caacaagtat tatgaaaaaa tcattcggct gctcgacgaa aatctttctt
    14401 cagaagatga ttgggcaatt tttcaggcca atttcgaccg aattcacgaa aactttttcc
    14461 gacatctaca cgaacgatat cccgaactca cacccaacga tttaagattt tgtgcttacc
    14521 ttcgcctcaa tctatcgagc aaggacattg cccatttaat gaatatttcg ctcaaaggcg
    14581 tagaaatggg aagagcccgc atccgcaaaa aaattggact cccttcaaca aaaagtctca
    14641 ccgaatttat gattgaattt aaataataac tgtcaatttt cacaaaaagg taccttgtta
    14701 ttatgcggtt taccagccgt tcggcgtagc ctcaaagcta cttcgagtta aaaataaaat
    14761 ctccagattt tcaataagcg aataaggttt tggatggtag gaggatttgc ctgttatgaa
    14821 ggtttttttg aaaaataagg tttaaaaaat cgactttgtt cggtgaaccg aagaaatata
    14881 cttctgagtt ctgagtttga gaatcgaatg ttagacaata aaaaaggaat tgaccttgtc
    14941 cggtgagccg aaaaatcatt gaagttggcg ttaaaaatct tttctgtttg agcgacggag
    15001 gagcgagttt agaa
//
LOCUS       DAOSIB010000057        14453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_4286_length_14453_cov_12.995902, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000057 DAOSIB010000000
VERSION     DAOSIB010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14453
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4286_length_14453_cov_12.995902"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="PLD44_06190"
     CDS             <1..1419
                     /locus_tag="PLD44_06190"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOF98958.1"
                     /translation="LDEKKAELKLKETYLNYLTKYLTGNFEEGTVVAPSNLGLNEPLL
                     MQLVQQINELYLQRSELTEKNLYYAKYTKAIENVKTSIFELIKNMRASLEIEKNEVNR
                     LLADVNSQIAELPKKELEIIGIERNYKVNDNYYTFFLQKRAEAEIQKASNTPDNNILD
                     QARTVSLINADVKSNTMFMFILLGILIPSLIIVLKEVLNNTIRSIKDIEKRSVFPVIG
                     MIRHAKNDLPDLTLKNPKSSFTEMFRVIRTRVEFVVRRKTDIVMMVTSVDAGDGKTFF
                     STNMAGVYGMTGHKTLLIDMDIRKPAINRRFNVNHPKGVTNYLINECTLDEIINVVPE
                     VNYDVIFAGTVPPNPAELIRSEKIIEMMGELRKRYDYIILDSSPIGLVTDSFSLASLA
                     DVNLLVVRNNKTHKDFFKNVVNQIVTDKLSNFYVVFTDVETDGNYYSYKYGYSSKYGY
                     YTSKGQKNIDDYRKYYDDNNEI"
     gene            1459..2613
                     /gene="gmd"
                     /locus_tag="PLD44_06195"
     CDS             1459..2613
                     /gene="gmd"
                     /locus_tag="PLD44_06195"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOF98959.1"
                     /translation="MENKKVALITGITGQDGAYLADFLLKKGYLVHGIKRRSSLFNTD
                     RIDHIYQDPHEQNRNFVLHYGDMTDSLNLTRIIQETQPDEIYNLAAMSHVKVSFDTPE
                     YTANADGIGTLRLLEAIRLLGLTEKTKFYQASTSELYGLVQETPQTEKTPFYPRSPYA
                     VAKLYGYWITVNYREAYGIHASNGILFNHESPLRGETFVTRKITRAASRIALGLQKNL
                     FLGNLSSKRDWGHARDYVKAMYLILQQDQPDDYVIATGKTTEVREFVRLAFEQMGLTI
                     AFEGKDLNEKGYIVQIDEPLFVQKIGEEFLSLVKSLKHPVIEVDPTYFRPTEVDLLMG
                     DATKARTKLNWQPEYDLEALIKDMMQSDIKLMKKELHLKNGGFKVLNYFE"
     gene            2628..3689
                     /locus_tag="PLD44_06200"
     CDS             2628..3689
                     /locus_tag="PLD44_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOF98960.1"
                     /translation="MDKNSKIYIAGHKGMVGSAIMRNLQNKGFSNIVGRTREELDLLN
                     QISVARFFDEEQPEYVVLAAAKVGGIVANNTLRAQFIYENLMIQNHVIHQSYLHGIKK
                     LLFLGSSCIYPRLAKQPIKEEYLLTGELEPTNEPYAIAKIAGIKMCEAYHAQYGCNFI
                     SVMPTNLYGPNDNYDLEKSHVLPALIRKMLLGKYLMENDFESLRKDFQKHPIEGVNGT
                     YSEEILLKVLEKYGISRKGQEVSITLWGTGSPLREFLHVDDMADAAVHLFFHYDAPDT
                     VPSHVNIGCGEDIRIKDLAEIIKKIVGYEGKIVWDASKPDGTPRKLLDVSKLNALGWK
                     PKITLEDGIRQVVNNYVFS"
     gene            3729..5063
                     /locus_tag="PLD44_06205"
     CDS             3729..5063
                     /locus_tag="PLD44_06205"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOF98961.1"
                     /translation="MEIAIVGTGYVGLVTGTCFAEMGIDVTCVDIDEQKIEKLKSGII
                     PIYEPGLGDMVLHNVKAGRLHFTTKLTDVLDDVQVIFSAVGTPPGEDGSADLRYVLDV
                     ARTIGQNMNQYVLLVTKSTVPVGTAKLVRQTIQNELDKRGVSIEFDVASNPEFLKEGD
                     AIKDFMSPDRVIVGVESEKAREIFARLYRPFLINNFRVIFMDIPSAEMTKYAANSMLA
                     TRISFMNDIANLCEIVGADVNMVRKGIGTDQRIGSKFLYAGCGYGGSCFPKDIKALIQ
                     TAREKGYEMKVLKAVEEVNNTQKEILYRKLIKHLGENLEGKTIAIWGLSFKPNTDDVR
                     EAPALLLTEKLAKAGCHVRVYDPVAMPEAKTALSKTLPEFLDKIHFASDIYDAALESE
                     ALLLVTEWKEFRMPSWGVIRKAMKNPLILDGRNIYDKKELQELGFTYEGIGV"
     gene            5109..5951
                     /locus_tag="PLD44_06210"
     CDS             5109..5951
                     /locus_tag="PLD44_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF98962.1"
                     /translation="MDNITETVIKPKSSLLEIDFKELWRNRDLFSMFVKRNIVTQYKQ
                     TILGPAWFFIQPALTTIMYMVVFGGIAGIPTDGLPQPMFYLAGIVCWQYFADCLTKTS
                     STFIDNQNIFGKVYFPRLIVPLSTVASNLVRMFIQFLLFVAVYVFYLIKGVHVEPNIY
                     ILLVPVLIIMLAGLSLGFGIIISSLTTKYRDLTILFNFIVQLWMYATPIIYPLSTMPP
                     DRQWIMALNPLTSIIEAFKYGTMGVGTFSWGQLAYSFGFMVVLLAVGIVIFNKVQRTF
                     MDTI"
     gene            6134..7060
                     /locus_tag="PLD44_06215"
     CDS             6134..7060
                     /locus_tag="PLD44_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HOF98963.1"
                     /translation="MTLQQLEYILAVYKTGHFMKAAELCQVTQPTLSAMIQKLEEELD
                     CKIFDRSQHPIQPTEMGEKIIAQAQIILYQTNQLKEMLRKEKETVSGNLNLAIIPTIA
                     SYLLPRFIALFRNDYPEVSLKITEMRTDTIIQKLKTAEIDMAILATPLNNPDLLEIPV
                     YYEKFIAYISPGEPIYELEELSANNLPLEHLWVLEEGHCFRSQIFNFCDQMKVHNYMY
                     EAGSIETLVKIVDINGGYTVIPELHVESLTEEQKKNLRNIVQPEATREISIVIRNDYV
                     REGLLNAVADSLKKIIPPHMLDSRLKKFAIKI"
     gene            complement(7057..9006)
                     /locus_tag="PLD44_06220"
     CDS             complement(7057..9006)
                     /locus_tag="PLD44_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOF98964.1"
                     /translation="MDIGKNAFYFEDYVLSIQYFNQVIKIKPYLSEPYIYRAMAKIQL
                     GDYQGADEDCTQAIEKNPFVPQAYYARGFARLKMNFCNEAIDDFSKALEFSPGNIYLL
                     GYRLMARNQCNDYAGALNDVNEYLKLSPKSTEMYYEKGRILLALKDTVGAEQCFDKYI
                     QLNENSSLGWSARALLKMQKKDKKGALKDYDEAIKRNSTYSGDYINRGVLNTENKNFR
                     QALEDYDKAISLDKNNELAYFNRGLLRASLGDKNNALADFTKVLELNPSNTDALWQKA
                     TVENALGNYSQSIKDFSKIIEQYPYFIPAYSGIAEAYQKLGDEKNAFRYRQMAYNIEQ
                     NKNNIRKEELAAGNKIAKETQKNVSEKNLDFFNRFAVQDKNDTAGESKYNNNIRGAVQ
                     NRYADVVNEKNFVLTYYSKADEIRRTNLFHLSIERYNSQKKLPLPLKITNNELPLTAE
                     MISQHFDAINNISAQLEQNSSDADIYFARAIEFALVQDFKSAIDDLNKSISIKPDFMP
                     AYFTRANIRYKMIETNSNGNLGGSANKPVNDIGSNNQLKFDMELILRDYDKVIELVPD
                     FTFAYYNKANVLTAIRDFRSAVSYYSKAIDIDPDFAEAYFNRGLTYLFIGDDKNGLSD
                     LSKAGELGIYKSYNLIQRFKQQKEN"
     gene            complement(9104..11422)
                     /locus_tag="PLD44_06225"
     CDS             complement(9104..11422)
                     /locus_tag="PLD44_06225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOF98965.1"
                     /translation="MENQNNDKRTKFKKIFWNLFGFGILCLVLLFAFISFGWIGYLPP
                     LEELQNPQNKYASEIYSSDMQLLGRFFKNKENRVEVHFNDISKNVINALIATEDKRFY
                     KHSGIDGKALARAILLTGILQQKSAGGGSTITQQLAKQLYSPQADNIFERMLQKPIEW
                     VISVKLERNYTKEEILTMYLNQFDFLYNAVGIKSAAQIYFSTSPSQLKIEEAATLIGM
                     CKNPAYFNPVRYNERTRERRNMVLYQMYKCDYLTKQQYDSLKNLPLVLHFQRIDHKEG
                     LAPYFREYLRTTLMAKEPHRSDYASWQDQQYLDDRWAWDNDPLYGFCNKNLKSDGTPY
                     NIYTDGLKIYTTIDSRMQRYAEEAVNEHIQTLQAAFFKEKKNRSYAPFSSSLSPKEIE
                     DILNRSIRQSERYSKMKAQGKSDAEIMEEFKKPVEMRVFSYDGDIDTIMSPLDSIRYH
                     KHFLRCGFMSMEGRSGFVRAYVGGPNFNRFQYDMVNKGRRQVGSTIKPFLYTLAMEEG
                     MWPCDQTVNQPITFRTATGELWTPRNSSKARIGETVTLRWGLANSNNWISAYLMSLFT
                     PESLVKLMRSFGIRGHIDPVVSLFLGPCEISVEEMVDAYTAFMNKGIRVEPVYVTHIE
                     DANGNVIGKFSPKMHEIFSETTAYKMIYMLQGVIDGGTGSRIRTRFGLTMPMGGKTGT
                     TQNNSDGWFIGFTPSLASGVWVGGEERSIHFDRMSEGQGASMALPIWALYMKKVLADS
                     TLNYSPGENFEIPSWFNPNAGCEDSSNGNSNE"
     gene            complement(11474..12601)
                     /gene="hemW"
                     /locus_tag="PLD44_06230"
     CDS             complement(11474..12601)
                     /gene="hemW"
                     /locus_tag="PLD44_06230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOF98966.1"
                     /translation="MAGIYIHIPFCIQRCSYCNFFSVVSINLVNEVINAIIKELSIRK
                     DYLKNEPVQTIYFGGGTPSLLTIQQIEKLLNTVYTNFPVEQNAEITFEANPDDLTKNY
                     LFDLKKVGINRLSIGIQSFDDDKLKILNRRHNATQAHEAVREAQKAGFSNISIDLIYG
                     LPFQSLDEWETDLDKAFSLSIQHLSAYGLTFEEKTLLSKQKEKKIIEPADEELMIKMY
                     ELLLEESSKKGFDAYEISNFSLPGFYSRHNSSYWNQTPYLGVGPSAHSYNGISRQWNV
                     AAIQKYTEGILKNSSFYEQEILSLEDRYNEYVLLGLRTKNGINIDALEQNFGTELKDF
                     CLENIKTFIDNEKAYRCGSFLRLNTKGKLISDTIIAQLMKV"
     gene            12722..12796
                     /locus_tag="PLD44_06235"
     tRNA            12722..12796
                     /locus_tag="PLD44_06235"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12757..12759,aa:Pro,seq:tgg)
     assembly_gap    13059..13158
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13233..13838)
                     /locus_tag="PLD44_06240"
     CDS             complement(13233..13838)
                     /locus_tag="PLD44_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98967.1"
                     /translation="MKSNLTSILMTIGVVLMLIAAYIYLFRQLPKEDISSEKYQEAYH
                     AKPSASVSQLPVYKPSYSSRKRAVVFPNSIHESDKLTGTLNNTNNDLGSEPIGVIPPI
                     DISYALPPSRELYKGVNYNYYLKGNLTHWQKISRSSVTAPFSGERRELAQNIEISSSR
                     PQRVGGDTDPGEPGLPVGDEFHFLLCLAFIYGGIKHFISRK"
     gene            complement(13996..>14453)
                     /locus_tag="PLD44_06245"
     CDS             complement(13996..>14453)
                     /locus_tag="PLD44_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98968.1"
                     /translation="PLAVYTKTSGSHTFHAVASQSSTLAELTLTDKTAGKSVNLLTSD
                     YTYSATAGTNTTRFLISARRVISSTLPQEKRNNLVVFSTNGKIVVDGLSVNSLVRVFD
                     ITGKPMAIVSAAVADKLEIPLSVAGVYQVVVESPNQIRKTIKIMHYPSR"
BASE COUNT         4341 a         2626 c         2861 g         4525 t
ORIGIN      
        1 ttggatgaaa agaaagccga actaaaactc aaggaaactt atctgaatta tttgaccaaa
       61 tatctcacgg gtaattttga agaaggaacg gttgttgctc catcaaatct cgggctaaat
      121 gagcctttgt taatgcaatt ggttcaacag atcaacgaat tatatttaca acgaagcgaa
      181 ttaaccgaaa aaaatctgta ttatgccaag tataccaaag ccatagaaaa tgtgaaaact
      241 tcaatttttg aattgataaa aaatatgagg gcttcgttgg aaatcgaaaa aaatgaagtc
      301 aatcgccttc ttgcagacgt taacagccaa atagccgaac tccctaaaaa agaattggaa
      361 attattggaa ttgaaagaaa ttacaaggtc aacgacaatt attatacttt tttcctgcaa
      421 aaaagagctg aagccgaaat tcaaaaggct tccaatacgc cggacaataa cattctggat
      481 caagcccgta ccgtatctct gattaatgca gatgtcaagt ctaataccat gtttatgttt
      541 attttacttg gtattttgat cccttctttg ataattgtgc taaaagaagt tttgaacaat
      601 accattcgtt cgataaaaga tattgaaaag cgatcagtgt ttccggtgat tggtatgatt
      661 cgtcatgcaa aaaacgactt gcctgatctg actctgaaaa atcccaaatc ttcctttacc
      721 gaaatgttca gggtaattcg tacacgagtg gagtttgtag tgcgtcgcaa aacagacata
      781 gtcatgatgg ttacttcggt ggatgctggc gacggtaaaa catttttctc gaccaatatg
      841 gccggagttt atggaatgac cggtcataaa actctattga ttgatatgga catcagaaaa
      901 ccggccataa acaggcgttt caatgtaaat catcccaaag gagttaccaa ttatctgatt
      961 aatgaatgta cgctggatga aattattaac gtcgttcctg aggttaatta cgatgtgatt
     1021 tttgccggta cagtcccacc aaatccagct gaattgatcc ggtctgaaaa aattatagaa
     1081 atgatgggtg aacttcggaa acgatacgat tacatcattc tcgattcgag cccaataggc
     1141 ttggttaccg acagtttctc actggcatcg ttggccgatg ttaatttgct ggtggtgcga
     1201 aacaataaaa cacataaaga ctttttcaaa aatgttgtga atcagattgt aaccgacaaa
     1261 ttgagcaatt tttatgtggt atttaccgat gtagaaacag atggcaatta ctattcctat
     1321 aaatatggat attcgtcaaa atatgggtac tatacttcaa aaggacaaaa aaacattgat
     1381 gattaccgta aatactacga tgataataat gaaatataaa taagctacaa aaaatttatt
     1441 tgtcaaacaa ttagcattat ggaaaacaaa aaagtagcat taattacagg tattacgggt
     1501 caggatggtg cttatctggc tgattttctt ttgaaaaaag gctatttggt acatggaata
     1561 aaacgtcgtt cttcattgtt caataccgac agaattgatc atatatatca ggatcctcat
     1621 gaacaaaacc ggaattttgt tttgcactat ggcgatatga ccgattcgtt gaatctgacc
     1681 agaattattc aggaaactca acctgatgaa atctacaatc ttgctgccat gagccatgta
     1741 aaagtgagtt ttgatacacc ggaatacacc gccaatgccg atggaatagg aactttacgt
     1801 ttgctggaag ctattcgttt attgggactt accgaaaaaa caaaatttta tcaggcttct
     1861 acttccgagt tgtatgggtt ggttcaagaa actccacaaa ccgaaaaaac tcctttttat
     1921 ccccgttcgc cttatgctgt agcaaaactt tatggttact ggattacggt aaattataga
     1981 gaagcttatg gtattcatgc cagcaatggc attttgttca atcatgaatc gccattgcgt
     2041 ggtgaaacct ttgttacccg aaaaattacg cgtgctgcat ccagaattgc actgggcttg
     2101 caaaaaaatc tttttctggg aaatttgtcc tccaaacggg attggggaca tgcccgcgat
     2161 tatgtgaaag caatgtattt gattttgcag caggatcaac ccgatgatta tgtaattgcg
     2221 acgggaaaaa ccacagaagt cagggaattt gttcgattgg cttttgaaca gatgggcttg
     2281 acgatagcat ttgagggaaa agatttgaac gaaaaaggat acattgtgca aatagatgag
     2341 ccactttttg tgcaaaaaat tggagaagaa tttttgtcgt tggtaaaatc tcttaaacat
     2401 cctgtgatag aagttgatcc aacctatttc cgtccgactg aagtggattt gttgatgggt
     2461 gatgcaacga aagccagaac caaattgaac tggcaaccgg aatatgattt ggaggcattg
     2521 atcaaggaca tgatgcagtc ggatattaaa cttatgaaaa aggagctaca tttaaaaaat
     2581 ggcggtttta aagtgctgaa ttattttgaa taattataaa atttacaatg gataaaaatt
     2641 ccaaaatata tatagccgga cacaaaggga tggtaggttc ggcgattatg cgaaatttgc
     2701 aaaataaagg attttcgaat atagttggac gaaccaggga agaactcgat ttgttgaatc
     2761 aaatttcggt tgcccgtttt tttgatgaag aacaaccgga atatgttgtg ctggcagctg
     2821 ccaaagtggg cggaattgtg gccaacaata ccttacgtgc ccagtttatt tatgaaaatt
     2881 tgatgataca gaatcatgtc attcatcaaa gttatctgca tgggataaaa aaactcttgt
     2941 ttcttggctc ttcgtgcatt tatccacgtt tggccaaaca acctattaag gaagaatacc
     3001 tgctaacagg tgagctggag ccaaccaacg agccttatgc cattgcaaaa attgcaggca
     3061 taaaaatgtg tgaagcttat catgctcagt atggctgtaa ttttatttcg gtgatgccca
     3121 ccaatcttta cggccccaat gataattatg atcttgagaa atcgcacgta ttaccggctt
     3181 tgataagaaa aatgctgttg ggaaaatact tgatggaaaa cgattttgaa agtttgagaa
     3241 aagattttca aaaacatccc attgagggag ttaatggcac ttacagcgaa gaaattttgt
     3301 tgaaagttct tgaaaaatac ggtatctcac gcaaaggtca ggaggtttct attacacttt
     3361 ggggaaccgg cagtccactt cgtgaatttc tacatgttga cgatatggct gacgctgccg
     3421 ttcatttgtt tttccattac gacgcacccg atactgtgcc ttcacacgtc aatattggtt
     3481 gtggtgaaga tattcgaata aaagatttgg ctgaaattat aaaaaaaatt gtaggttacg
     3541 aaggaaaaat tgtttgggat gcatcaaaac cggatggtac tccacgaaaa ttactggatg
     3601 tttccaaact gaatgctttg ggttggaaac caaaaattac tcttgaagat ggaattcgtc
     3661 aagtagtaaa caattatgtt ttttcatgat tcggacattt ttaataggga aaaaattaac
     3721 ttgataatat ggaaatagct attgttggaa ccgggtacgt cggtttggtt accggtactt
     3781 gttttgccga aatgggaatc gatgttacgt gtgtggatat tgatgaacaa aaaatagaaa
     3841 aattgaaaag cggcataatt ccaatttatg aaccgggatt gggagatatg gttttacata
     3901 atgtcaaagc tggtcgtttg cattttacaa caaaactaac cgatgtgctg gacgatgtac
     3961 aggtaatttt cagtgctgta ggaacacctc ccggtgaaga tggaagtgcc gatttaaggt
     4021 atgttcttga tgtggcacgc acgatagggc aaaacatgaa tcaatatgta ttgctggtaa
     4081 ccaaaagtac ggttcccgta ggaacagcaa aacttgttcg ccaaactatc cagaatgaat
     4141 tggacaaacg cggggtgtca attgaatttg atgtggcttc caatcccgaa tttctgaaag
     4201 aaggtgatgc cataaaagat ttcatgagtc ccgacagagt tattgtgggt gtggaaagcg
     4261 agaaagcacg cgagattttt gcccgcttgt accgtccttt cctgatcaac aattttcgtg
     4321 ttatttttat ggatattcct tccgccgaaa tgacaaaata tgcagctaat tcaatgctgg
     4381 caacacgtat cagttttatg aacgatattg ccaacctttg cgaaattgtg ggtgccgatg
     4441 ttaatatggt aagaaaaggt attggtaccg atcagcgcat tggcagtaaa ttcctgtatg
     4501 ccggctgtgg ttacggtggt tcctgctttc caaaagacat taaagctctt atacagactg
     4561 caagggaaaa aggatacgaa atgaaagtat tgaaagctgt agaagaagta aataatactc
     4621 agaaagaaat tctttaccgt aaattgatta agcatttggg tgaaaatttg gaagggaaaa
     4681 ccattgccat ttggggatta tcatttaagc ccaatacaga tgatgttcgt gaagctccgg
     4741 cgttgttgtt gacagagaaa ttggcaaaag ccggatgcca tgttcgtgtt tacgatccgg
     4801 tggccatgcc cgaagcaaaa acagcgttga gcaaaacttt gcctgaattt cttgacaaaa
     4861 ttcattttgc ttccgatatt tacgatgctg ctttagaatc ggaagctctg ttgctggtta
     4921 ccgaatggaa agaattcaga atgccgagtt ggggcgttat caggaaagca atgaaaaatc
     4981 ctttgattct tgatggacga aatatttacg acaaaaaaga acttcaagaa ttgggattta
     5041 cttatgaagg tattggagtt tgatgacaaa atatacataa tttgttgatt ttactgagag
     5101 aataatgtat ggataatatt accgaaacag ttatcaagcc taaaagcagt ttgttggaaa
     5161 ttgattttaa ggaattatgg agaaataggg atttattttc catgttcgtt aagcgcaata
     5221 tcgtaacgca atacaagcaa accattttgg gccctgcgtg gtttttcatt caaccggcat
     5281 tgacaaccat catgtacatg gttgtttttg gcggtatagc cggcatcccg accgatggat
     5341 taccacagcc catgttttat ttggccggca ttgtttgctg gcagtatttt gccgattgtt
     5401 tgaccaaaac ttccagtact tttatcgaca atcagaatat tttcggaaaa gtttattttc
     5461 cacgactgat tgttccactt tctaccgtcg cttccaacct tgtgcggatg tttattcagt
     5521 ttttgctttt tgttgccgta tatgtttttt atttgattaa aggcgttcat gttgagccga
     5581 atatttatat attgctcgtt ccggtgctta ttatcatgtt ggccggctta tcacttggct
     5641 ttggcataat tatttcttca cttaccacca agtatcgcga tttaactatt ttgttcaact
     5701 ttattgtaca gttgtggatg tatgctacgc caattattta tcctttaagc accatgccgc
     5761 ccgatcgtca atggattatg gcattgaatc cgctgacatc cattattgaa gctttcaagt
     5821 acggaactat gggcgtagga actttcagct gggggcaatt ggcttacagt tttggtttta
     5881 tggttgttct tctagcggtt ggaattgtta ttttcaacaa agtgcagcga acattcatgg
     5941 acactattta gaaaaaaaat caaagctaat ttaattggtt aaaattgagt taggtataag
     6001 aatgttttat aagtaattaa aaattagtta ataaggaaga tgaaaaaagg ggaaaattct
     6061 cctattactg agattagttt tgtttagata aggtctttat atcgaactca cgttaattta
     6121 ataatttctc gacatgactt tacagcaatt ggaatatatt ctggcagtat ataaaacggg
     6181 tcattttatg aaagctgccg aattgtgtca ggtaactcag ccaactttga gcgccatgat
     6241 tcaaaaattg gaagaagaac ttgactgcaa gatatttgac cgctcgcagc atcccattca
     6301 gcccaccgaa atgggagaaa aaattattgc tcaggctcaa attattcttt atcagaccaa
     6361 tcagttgaaa gaaatgttac ggaaagaaaa ggaaactgtt tcgggaaatt tgaatctggc
     6421 aattattcca actattgctt cctatctttt gccgcgtttt attgccttgt tcagaaacga
     6481 ttatccggaa gtctcgttga aaataactga aatgcgtacc gacaccatta ttcagaagtt
     6541 gaaaactgcc gaaattgata tggcgatatt ggcaacgcca cttaacaatc ctgacttgct
     6601 cgaaattcct gtttattacg aaaaatttat tgcctatatt tctcccggcg aaccaattta
     6661 tgagttagaa gaattgtcgg ccaacaattt gccgttggag catctttggg tgctggaaga
     6721 aggtcattgt ttcagaagtc agattttcaa tttctgcgac caaatgaaag ttcataatta
     6781 catgtacgaa gccggcagta ttgaaacatt ggtgaaaatt gtggatatca acggaggtta
     6841 tacggtaatt cccgaattgc atgttgagtc acttactgaa gagcagaaaa agaatcttcg
     6901 aaatatcgtt caacccgaag ccaccagaga aatttccatc gtcattagaa acgattatgt
     6961 tcgcgaaggc ttgttgaatg ccgttgctga cagcctgaag aaaataatac ctcctcacat
     7021 gctggatagc cgcttgaaaa aatttgccat taaaatttag ttttctttct gctgcttgaa
     7081 tctttgaatc agattatagc ttttataaat tcccaattct ccggctttgc tcaaatccga
     7141 cagtccgttt ttgtcgtctc cgataaacag ataggttaat ccccgattaa aataggcttc
     7201 tgcaaagtcg ggatcaatat ctatggcttt gctgtagtaa cttacagccg aacgaaaatc
     7261 gcgaatggca gttaaaacat tggctttgtt gtaataagca aatgtgaaat cgggaaccaa
     7321 ttctatgacc ttatcataat ctctcagaat taattccata tcaaatttca attgattatt
     7381 cgaaccaatg tcgttaaccg gtttgttggc agaaccaccc aaatttccat tggaatttgt
     7441 ttcaatcatt ttgtatcgaa tatttgctcg ggtaaaataa gccggcataa agtcgggctt
     7501 gatcgaaatg gatttgttca aatcatcaat agcgctttta aaatcctgta ccaaagcaaa
     7561 ttcaatggct ctggcaaaat aaatatctgc atccgatgag ttttgttcga gttgcgcaga
     7621 aatattgttg atagcatcga aatgctgact aatcatctcg gctgtcaggg gtaattcgtt
     7681 attggtaatt tttaagggca gcggcagttt tttctggctg ttgtagcgtt caatggacag
     7741 atggaaaaga ttggttctgc gaatttcatc agctttactg taataagtca gcacaaaatt
     7801 cttttcgttc acaacgtctg catatcggtt ttgtactgct ccacgaatgt tgttgttata
     7861 ttttgattcg ccggcagtat catttttatc ctgcacagca aaacgattga agaaatcgag
     7921 attcttttca gaaacatttt tctgggtttc ttttgcaatt ttattgccgg cagccaattc
     7981 ttccttcctg atgttatttt tgttttgttc tatgttataa gccatttgcc tgtatctgaa
     8041 cgcatttttc tcatcaccaa gtttctgata ggcttcggct attccagagt aggccgggat
     8101 aaaataagga tattgttcga taatttttga aaaatctttt atggactgcg agtaatttcc
     8161 cagtgcattt tctactgtag ctttttgcca gagagcgtcg gtattggatg gattaagttc
     8221 caaaactttt gtaaaatctg caagtgcatt atttttatct ccaagactgg ctctgagtaa
     8281 acctctgttg aaataagcca attcattgtt tttgtccagt gaaattgctt tatcgtaatc
     8341 ttccagagct tgcctgaaat ttttgttttc cgtgtttaat acgcctctgt taatgtaatc
     8401 tccgctgtag gttgaatttc tcttgatggc ttcatcataa tctttcaaag ctcctttttt
     8461 gtcttttttc tgcattttca ataaagcacg agcactccac cccaagctgc tattttcgtt
     8521 caactgaatg tatttgtcga aacattgctc ggctccaacc gtgtctttta atgccaacag
     8581 aattctgccc ttttcataat acatttccgt acttttgggg cttaatttca gatactcgtt
     8641 tacatcattc aaagcacctg cgtaatcatt acattggttg cgtgccataa gccgatagcc
     8701 aagcaaatat atatttcctg gactaaattc cagcgctttc gagaaatcat cgatagcttc
     8761 gttacaaaaa ttcattttca atcgggcgaa acctctggca tagtaggctt gcggaacaaa
     8821 aggatttttt tcaatagctt gcgtacagtc ttcgtcggcg ccctgataat cgccgagttg
     8881 aatttttgcc attgcccgat aaatataagg ttccgacaaa tacggcttaa ttttgattac
     8941 ctgattgaaa tactgaatgg aaagtacata atcttcaaaa taaaaagcat ttttccctat
     9001 gtccataatt ctatcggtat tccattgagc atgggcggca aaggaaaata ataatcctgt
     9061 gaagcacaga agaaattttt ttctaaagtg gacgaatcta ttattattca ttactatttc
     9121 cattactgct atcttcacaa ccggcattgg gattgaacca ggaaggaatt tcaaaatttt
     9181 ctccgggtga atagtttaaa gttgaatcag ccaaaacttt tttcatgtac aaagcccaaa
     9241 ttggcaaagc catacttgca ccttgccctt ccgacattct gtcgaaatga atggaacgtt
     9301 cttcaccacc aacccaaacc cccgaagcca gtgaaggagt aaatccgatg aaccaaccgt
     9361 ccgaattgtt ttgtgtcgtt cccgtttttc ctcccatcgg catggttaat ccgaaacgtg
     9421 ttctgattct cgaaccggtt cctccgtcaa taactccctg tagcatgtaa atcattttgt
     9481 aagccgtagt ttcactgaaa atttcgtgca ttttcggaga aaattttcca attacatttc
     9541 cgttggcatc ttcaatgtga gttacataaa ccggttcaac tctaattcct ttattcataa
     9601 atgcagtata ggcatccacc atttcttcaa cggaaatttc gcaaggtccc aaaaacaatg
     9661 atacaacggg atcaatatga cctctaatgc caaaggaccg cataagtttt acaagcgatt
     9721 cgggagtgaa aagactcatt aaataagctg aaatccagtt gtttgaattg gcaagtcccc
     9781 aacgcagggt aactgtttct ccaattctgg cttttgaaga gtttcttggt gtccaaagtt
     9841 caccggtggc tgttctgaaa gtaatgggct gattaactgt ttgatcgcaa ggccacattc
     9901 cttcttccat tgccaatgtg taaagaaacg gtttgatagt tgaacccact tgtctgcgtc
     9961 ctttgttgac catatcgtat tggaaccgat tgaaattcgg gccaccaaca taagctctga
    10021 caaatccgct gcgtccttcc atcgacatga aaccacaacg caagaaatgt ttgtggtagc
    10081 gaatggaatc gagaggagac ataatggtgt cgatatcacc atcataagag aaaactctca
    10141 tttctaccgg tttcttgaat tcctccataa tttcggcatc gcttttacct tgtgctttca
    10201 ttttagaata tctttccgat tgccggatcg atcggttcaa aatatcttct atttctttgg
    10261 ggctcaggct actggagaaa ggagcataac ttctgttttt cttttctttg aaaaatgcag
    10321 cctgcaaggt ttgaatgtgt tcatttacag cctcttctgc atatcgctgc atacgagaat
    10381 caatggtggt ataaattttc aaaccatcag tatagatatt gtacggcgtt ccgtctgatt
    10441 tcagattttt attgcagaat ccatacaaag ggtcattgtc ccaagcccag cgatcatcga
    10501 gatattgttg gtcttgccag ctggcataat cgcttcgatg aggttctttt gccattaatg
    10561 tggttctgag gtattctctg aaataggggg ccaatccttc tttgtgatcg attcgctgaa
    10621 aatgaagcac caacggaaga ttttttaatg aatcatactg ttgtttagtg agataatcgc
    10681 atttgtacat ttgatataaa accatattgc gcctttcgcg tgtgcgttca ttgtaacgta
    10741 cgggattaaa gtaggcggga tttttgcaca ttcctatcaa agtggcagct tcttcaattt
    10801 tcagttgaga aggactggtg ctgaaataga tttgagcagc cgattttatt cctacagcat
    10861 tgtaaaggaa atcaaactga ttaaggtaca tggtcagaat ttcttctttt gtgtagttcc
    10921 gttccagttt gacagatatt acccattcaa tgggtttttg aagcattcgt tcaaaaatgt
    10981 tgtcggcttg aggtgaataa agttgcttgg ccaattgttg ggtaatggtg cttcctcctc
    11041 cggcgctttt ttgctgcaaa ataccggtaa gtaaaatagc tcgggcaagc gctttgccgt
    11101 cgatgccgga atgtttgtaa aatcttttgt cttcagtagc aatcaaagca ttaataacat
    11161 ttttagaaat gtcgttgaaa tgtacttcta cacgattttc cttgttttta aaaaatctac
    11221 ccagcaattg catgtctgaa gagtaaattt ccgaagcata tttgttttga ggattttgta
    11281 attcttcgag tggaggcaag taaccaatcc agccaaaact aatgaaagca aagagcaaaa
    11341 ccaagcaaag gatgccaaat ccaaataaat tccagaaaat ttttttaaat ttagtacgct
    11401 tgtcgttgtt ctgattctcc atatttttta atctaacgaa taagttacaa aattagtaat
    11461 aattttccat aacttaaacc ttcattaatt gagcaataat ggtgtcggat attagttttc
    11521 cttttgtgtt taaacgtaaa aaactgccgc acctatatgc cttttcatta tcgataaatg
    11581 tttttatgtt ttctaaacaa aagtctttca attcggtacc aaaattttgt tccaacgcat
    11641 caatatttat accgtttttt gttctcaatc cgagtaatac atattcatta taacgatctt
    11701 ccaaactgag aatttcctgt tcgtaaaagg aactgttttt caaaattcct tctgtatatt
    11761 tctgaatagc agcaacattc cactgccgag aaatgccatt ataggaatgt gcggaaggtc
    11821 ctacgcccaa atatggagtt tgattccaat aggaagaatt atgtcgtgaa taaaatcccg
    11881 gtaaggaaaa attggatatt tcgtaagcat caaacccctt tttgctgctt tcttccaaca
    11941 aaagttcata catttttatc atcaattctt cgtctgccgg ttctatgatt tttttttctt
    12001 tttgtttcga taaaagtgtt ttttcttcaa aggttaggcc ataggcagaa agatgttgaa
    12061 ttgacagtga aaaagcttta tccaaatcag tttcccattc atcgaggctt tggaagggta
    12121 atccatatat taaatctatg ctgatattgc taaaacctgc cttttgagct tcacgaactg
    12181 cttcatgagc ctgtgtcgca ttgtgtcttc tattgagtat tttaagttta tcgtcatcaa
    12241 atgattgaat tccaatactt aaccgattga tgcctacttt tttcaggtcg aacaaataat
    12301 tttttgttaa atcatcagga ttggcttcaa acgttatttc tgcgttttgt tctaccggga
    12361 aatttgtata aacagtattc agtaattttt caatttgttg aatcgtcaac agactgggtg
    12421 ttcctccgcc aaaataaatc gtctgtaccg gctcattttt caaatagtct tttctgatcg
    12481 acaattcttt aattattgca tttataactt cattaacaag attgatagag actacggaaa
    12541 aaaaattgca ataggaacat cgctggatgc aaaaaggaat atgaatgtaa ataccggcca
    12601 tttttaaagc gggaaagaaa ttaggaacaa ttcaagaatt cttttataca aaaatacagc
    12661 aaaaagttgg tattttcggc aaaagatttt aactttgcag tcgtttttaa aaaaatcagt
    12721 acggggtgta gcgcagtccg gttagcgcac ctgctttggg agcagggggt cgtgggttca
    12781 aatcccgcta ccccgacaca atagaagaac gatatttaaa agatttacaa ggttagcgca
    12841 tccagccaaa ggctggaggg tcgtgggttc aaatcccgct accccgacac aatagaagaa
    12901 cgatatttaa aagatttaca aggttagcgc atccagccaa aggctggagg gtcgtgggtt
    12961 caaatcccgc taccccgaca caatagaaga acgatattta aaagatttac aaggttagcg
    13021 catccagcca aaggctggag ggtcgtgggt tcaaatccnn nnnnnnnnnn nnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnnnnn nnnnnnnngt attgaaaaac tccacactca caactcgcat cccgtaacta
    13201 tgaacaaaat aaggttacag aaagttgttt tactattttc gggatataaa atgctttatt
    13261 ccaccataaa taaaagccaa gcacaataaa aaatgaaatt catctcctac cggcaatccc
    13321 ggttcgcccg gatcggtatc gcctcctacc cgttgcggtc gcgaagacga tatttcgatg
    13381 ttttgtgcca attcccgtct ttccccgcta aaaggagccg taaccgacga acgggagatt
    13441 ttctgccaat gtgtaagatt gccctttaga taataattat agtttactcc cttataaagt
    13501 tctctcgatg gtggaagtgc ataagaaata tcaataggcg gaattacacc aattggttcg
    13561 gatcccaaat cgttgtttgt gttgttcaat gttcccgtta atttgtcgct ttcatgaatt
    13621 gaattcggga aaacaaccgc ccttttgcgt gatgaatatg atggtttata aacaggtagc
    13681 tgtgaaacag atgctgacgg tttcgcatga tatgcttctt gatatttttc agatgaaatg
    13741 tcttcttttg ggagctgacg aaaaagatag atataggcag caatcagcat caataccact
    13801 ccaatagtca tcaatataga agttaaatta gatttcatga acctttaatt ttttttttga
    13861 taatcgttta tgcattgaat caattttaga taaaacgggt ttttataaac gatataaagc
    13921 agttgttttt ttaaaaaatt tttttcaaaa ttcttttccc tacccgataa ttttatgcgt
    13981 ttttctccaa catggttatc gggaagggta atgcatgatt tttattgttt tgcgaatttg
    14041 attgggactt tccactacca cctgataaac accggcaaca gacaatggaa tttccaattt
    14101 gtcggcaacg gcagcgctta cgatagccat tggtttcccc gttatatcga atactcgtac
    14161 caaactgttg acagataagc catccaccac aatttttccg tttgtactga aaacaactaa
    14221 attgtttctc ttttcttgtg gaagggtgga agaaatcacc cttcgcgccg aaatcaggaa
    14281 acgggtagta tttgtacctg ccgtagcgga ataagtgtaa tcggaagtca ataaatttac
    14341 cgacttgccg gctgttttat ccgtaagagt aagttctgcc aaagtggaag attgagatgc
    14401 aacagcatgg aaagtatggc tgccggacgt tttggtataa acggccagtg gca
//
LOCUS       DAOSIB010000058        14043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_4436_length_14043_cov_9.814126, whole genome shotgun sequence.
ACCESSION   DAOSIB010000058 DAOSIB010000000
VERSION     DAOSIB010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14043
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4436_length_14043_cov_9.814126"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..1942
                     /locus_tag="PLD44_06250"
     CDS             224..1942
                     /locus_tag="PLD44_06250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOF98969.1"
                     /translation="MKNYVVSARKYRPTTFKSVVGQTALTNTLKNAIKSNHLAHAYLF
                     CGTRGVGKTTCARIFAKTINCEHLTEDFEACNECESCKAFNEQRSYNIHELDAASNNS
                     VEGIRSLIDQVRIPPQIGKYSVYIIDEVHMLSSSAFNAFLKTLEEPPAHAIFILATTE
                     KHKIIPTILSRCQIYDFNRISVSDIVGHLQYVAHEEGITVDVNGLNVIAQKCDGGMRD
                     ALSIFDQLVSLCGDNISYQDVLTNLNVLDYDYYFQLIEAFMKNDVNTSLLIFNDIIQH
                     GFDAHHFVSGLSSHFRDLLVSKDPQTIQLLEVGADIGKHYQMQAQQCPEDFIFKALKI
                     CNDCDLNYNISKNKRLLVEIMLIRLCQLVKQEDKLNGEEKNEPLKKVVDELKTNNVVL
                     AKNSPSSTKVQDSTSNPMSTEVDAKQVKTERPTSISIKNNVVSSPSSSYEAPQKEQPD
                     ILNEIFTQDDLQKAWAELAKTLVDEARMTAFMQSNRPELISNTEFRILVTNELQEKEL
                     KRLKPTILNFLKTALKNSNIKMSIQIQEDRSVQKGRTPEEKYKIMAEENPALAKLRND
                     LQLEID"
     gene            complement(2285..5017)
                     /locus_tag="PLD44_06255"
     CDS             complement(2285..5017)
                     /locus_tag="PLD44_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOF98970.1"
                     /translation="MKKILFTFSLLLCYLLSWGQIITTNPTFITKDYPGEIEIIYDAT
                     QGTAGLKDYTGDVYAHTGVITSSSTSPSDWKHAPVWGDNSPKYKLTSLGNNKWKLLIT
                     PNMAGYYNLSPGEVVRKLAFVFRNSTGTLQGKDIGGADIFVTVYESNFNVAFTFPTSN
                     QSVAIGSSIDFTVSSSMSAELKLLLNNTVLATQQAATSLTYSYNFSVAGDYTFIASAK
                     VDNNTVYDTLLVCVPEPVVNQTRPAGTKLGINYIDNTTVTLVFHAPNKTNVFLIGEFN
                     DWAQLNAYQLKKDGEYWWITLTGLTPGKIYAYQYLVDGTIRCSDPYTELVLDPWNDKW
                     INQYYTIYPNLKPYPEGKTEGLVATFQTAKPAYNWEITNFSMPAKENLVIYEMLLRDF
                     TTEKSLQAAIGKLDYLKNLGITAVELMPIQEFDGNNSWGYNPNHYFAPDKAYGTPEMY
                     KKFIDECHKRGMAVILDMVFNQASGICPFALLYWDSTKNRPAADNPWMNPVAPHQFSV
                     LNDFNHSYSGTKEYFKRVLQYWITEYKVDGYRMDLTKGFTQNSGTESTWDQTRINNLK
                     DYYYAVTDVKPDAMFILEHFVGGSEEDYLAAQGMYLWRNLNNAYSQAAMGFQSNSDFS
                     GMNTSPRRWVGYAESHDEERNFYKAKTYGNGTVKTDSLYRISRVPLVVAFATLMPGPK
                     MLWQFEEMGYDYSIDSFGGRTNPKPHAWGWLNLPHRKAAADACAKIISMRKLYPNAFL
                     NGNYSLQVAYNDWSQGRRIALSHADLNMVVLGNFQSDASITANPSFQRTGTWYNLLTG
                     ETLNVSNTNMTINMQPGQLLIFTDRNVNFTNDNREISYNVSDYVYPTLTSGKLFISST
                     ANIQSIRIYNLQGSLLKQQNNDIKEIDLSMLKKGFYLVELETANGKSAHKIIKE"
     gene            complement(5248..5415)
                     /locus_tag="PLD44_06260"
     CDS             complement(5248..5415)
                     /locus_tag="PLD44_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98971.1"
                     /translation="MVEWITYDATPYNKLTENVKSHHAQVMKKDDMPKILPWLYIAIG
                     NVKRLLLDVHY"
     gene            5684..7402
                     /locus_tag="PLD44_06265"
     CDS             5684..7402
                     /locus_tag="PLD44_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOF98972.1"
                     /translation="MKFSFLSSLSLIIVSANICLSGFAQCGKGEYTISAFPCLNCAVT
                     CQKPAGPEWIHNATFYQIYPQSFKDTNGDGIGDLKGVISQLDYVKSLGVDAIWINPFY
                     VSPFRDAGYDVADYYHVDPRYGTDQDAKDLFRIAKQKGLRIIVDFVPGHTSIDHPWFK
                     ASCSSTPTKYSNWYIWTNGTWFDDENGKYKANFIQGFCDRDGNYMINFFWHQPALNYG
                     YGQPDPKQPWQLSTTHPDVVALRTEMKNVMRYWLEMGCDGFRVDMAGSLVKNDPEGSI
                     KYFWQDIRKMLDQEYPDAFMVSEWSESATAVNAGFHADFMLWQEGYQDLFMHENSYFR
                     SAGIGSISHFMWRYNEQSTETNGKGYISLTVGNHDLERINNRGRNRADLEIIQVFNFT
                     MPNIPFIYYGDEITMRQLPLVTGKEGCYGTRAGARTPMQWDTTANLGFSTSAPERLYL
                     PVDSSRNAPNVLAQENDPDSFLNFMRKLVLLHKTEPALTNYAKFVPVYVQKNKYPFIF
                     MRIFGNEKLLVILNPDSKPATAIVEGNLKGKRKLLLGKLPVWKQNTGTTEIQVDGQSY
                     SVIKLK"
     gene            7415..8851
                     /locus_tag="PLD44_06270"
     CDS             7415..8851
                     /locus_tag="PLD44_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOF98973.1"
                     /translation="MKKVSRYYFIFSALVLLLLFSGCRSLQKNSKTSKTGFTPEAYTA
                     LYADKWSAINDSVKAGWKNHSGKPSNNAVPDTFAYAFNEWTLFYWDLYFTQIGLLKHN
                     ELKLAKGGINNLKAELDKRGFIPNANTDWGENRSQPPYFGIMVKEFYDFTKDKKWLKT
                     MYAAVLKEYKFWTDTSANRIEANTTELGLQRYFHHATETELLELYDQELTSRFDFPKD
                     IEKSKKLNIASQFAAEAESGMDFTPRFENRCSEFIAVDLNSNLYLYEKYLAYFEQELK
                     IGNGQQWLAKARKRKALIQKYCWNDERGLFLDYDFVNNRSSKVAAATAFSPLFAGLAS
                     KYQADRMRENLHLFESENGIVACESTDEKRIYQWDHISVWSPMQSLVIMALDNYGFKE
                     DACRIAMKHLDLITANFFNPNPSTYVDKSGKKFYRLPGGIYEKYTRFGTINDREYNAS
                     LMYGWSAGAFAFAYDYLIVNSQHNFTIK"
     gene            8848..10242
                     /locus_tag="PLD44_06275"
     CDS             8848..10242
                     /locus_tag="PLD44_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 140 protein"
                     /protein_id="HOF98974.1"
                     /translation="MKKIRILFIIAFLFISLFGQKILCQSELKITSDHHYLSYKNGDP
                     FFWLGDTAWELFHRLKLEEIETYLENRKQKGFNVVQAVILPEMDGLRVPNRYGDVPLI
                     DMDPTKPNEKYFQFVDTVVKLAAKKGIFMGLLPTWGDKVTQLWGEGPVVFNPENAYKY
                     GKWIGERYKSYKNIIWILGGDRPPMKDSADWRPVWRNMAKGIEKATNHSALISYHIWG
                     GEMSTSQYIHSEKWLDINMMQSGHGSGKDVPVWEWIKRDYCMKPTKPTLDSEPNYEDH
                     PVNPWPSWNPENGYFRDYDVRKQTYRSVFAGACGVTYGNHSIWQFRNERDPNINFAER
                     YWTEALDRPGAFQVGYLKKLIESRPSSSRIPDQSIILEGQGEKEEYICAFRNRSNDYA
                     MIYIPVGKTITIKTSFIKTKKLKISWFNPQTCETIQSIETINTGKMCVTTPTTGFGKD
                     WVLIVEKDLVNTAK"
     gene            10744..12045
                     /locus_tag="PLD44_06280"
     CDS             10744..12045
                     /locus_tag="PLD44_06280"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOF98975.1"
                     /translation="MLTAQNPICPPGLNIADPSARVWNDGRLYVYGSRDESFNYYCSF
                     DYWVLSTDDLIHWQYTPNVFASKGQYDQVPFNDNLLFAPDCMYKNGTYYLYYCQPDGI
                     QGVATSLSPIGPFTNAKEIYLKNKNQIDPAVFVDDDGQAYYMWGQFQGKIAKLKPNMT
                     EIDTTTIVDNLVTEKEHFFHEGGYMVKRNGIYYFVYAHVGRKNMPTCIGYSTSKSPLG
                     PFKYGGVIIDNDGCDPANWNNHGSIVEFKGQWYVFYHRTTNGCVNSRKACIEPIYFNT
                     DGSITEVEMTSQGAGKPLDAFSKIQAEHACLLSGHVRVESYSFDTNNPLNPTNSDQLG
                     QIEDKDRAMYKYIDFGEGVDSVSIRIAPGLNSGKISFILDNSWSLPFAEIAVDGGGDG
                     KTWKIFSCKVKRVNGIHALNLTFSSTGSNTFNIDWFTFKHQ"
     gene            12316..13839
                     /locus_tag="PLD44_06285"
     CDS             12316..13839
                     /locus_tag="PLD44_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98976.1"
                     /translation="MEKPSWDGEIGGDGAIDKKTGSVSPDVLAYLKEMKIPIIRYPGG
                     TDVDYYQWYNLIENVPGKHQKRPAYRNYSGTGKITSDNRLGLDAFLTLCSELKSEPLL
                     VVNIGDAFFRKISIEESAKNAAGFVAYCNSKVGTKVSEGMPPDWPAIRAKNGHTKPYN
                     VRYFEIGNEIQLFKSMKEKDVNDSLVNHYVNCLEAIVNAMLDVDPNIKIITDGEIKEV
                     SEQIKRRLGNKISYLACHYYLPWEINTVKLDTTTIDIKSLTQADIWNAWVATPYIHPE
                     TGQSYFPKTGKYNHAADAGYDLAITEWNWNGWWNKKILPDAALNSLFAKGIGAAGFIH
                     AMMRDAGRMKIGCQSMLVGNVWDINAIRVDTTGKMKARFFPTGKVTTLYSKYHGNQLL
                     EMSEKNQLFYTQPYKMNSIEAVSRVTYVDALASRNETTLYISFINRNFANDMDVEIDL
                     SDFKTDSKYKQHIFTTNDMITNELGYFEDKSEKVKNHKPLIVHLPKHSLSIFEIKIF"
BASE COUNT         4493 a         2458 c         2772 g         4320 t
ORIGIN      
        1 tttattcgga tatacatatt aaccattatt tccaacggta cgataatttt ccattaaata
       61 atccttcctt tatttttggt gagcagccta taaaaatcat tttttttatg taagtttgca
      121 tgaaaaagaa gaaaaacaat tggaattgca acaaaatatt tattccaaaa tgttttgata
      181 atggtcttaa aatcaacaac attgtgttta tttttacaac agtatgaaaa attacgttgt
      241 ttcggcgcgc aaatacaggc ctacaacttt caaatcggta gttggacaga ctgctctgac
      301 caatactttg aaaaatgcca taaaaagtaa tcatttggct cacgcttatc ttttttgtgg
      361 tacacgagga gtaggaaaaa caacttgtgc ccgcattttt gcaaaaacca ttaattgtga
      421 gcaccttacc gaagactttg aggcttgcaa cgagtgcgag tcatgcaagg cttttaatga
      481 gcagcgttcc tataacattc atgaattgga tgctgcttcc aacaatagtg tggaaggtat
      541 tcgttcgttg attgatcaag ttcgcattcc acctcaaatt gggaaataca gtgtgtatat
      601 tatcgacgaa gtacacatgt tgagttccag tgctttcaat gcttttttga aaacactgga
      661 agaaccgccg gctcatgcca tttttatttt ggcaaccacc gaaaagcata aaattattcc
      721 aaccattctt tcgcgttgcc aaatttatga ttttaacaga atttccgtat cagatattgt
      781 gggacatctg caatatgttg ctcatgaaga aggaataacg gtagatgtga acggactaaa
      841 tgtcatagct caaaaatgtg atggaggaat gcgtgatgcg ctctctattt tcgatcaact
      901 ggtaagcttg tgtggagata atatttctta tcaggatgtg ctcaccaatc tgaatgtttt
      961 ggactatgat tattattttc agttgataga agcctttatg aaaaatgatg tgaacacttc
     1021 tcttcttatt ttcaatgata taattcaaca cggttttgat gcacatcatt ttgtctccgg
     1081 actgagttct cattttcggg atttgttggt cagtaaagat cctcaaacca ttcaattgct
     1141 ggaagtgggt gccgatatag gaaaacatta tcaaatgcaa gctcagcaat gtcctgaaga
     1201 tttcatattt aaagccttga aaatttgtaa cgattgcgat ttgaattaca atatcagcaa
     1261 aaacaagcga ttgttggtag agattatgct gattcgtctt tgtcaacttg tcaaacagga
     1321 ggataaactc aatggggaag agaaaaatga acctctgaaa aaagtggttg atgagctgaa
     1381 aacaaataat gttgttctcg ctaaaaatag cccttcatcg actaaagttc aagattcaac
     1441 gtcaaatcca atgagtacag aagttgacgc aaagcaggtg aaaacggaac gtccgacaag
     1501 tatttccatt aaaaataatg ttgtttcttc tccttcatcg tcctatgagg caccacaaaa
     1561 ggaacagcct gatattctga acgaaatttt tactcaggat gacttgcaaa aagcttgggc
     1621 tgaacttgct aaaactttag tagatgaggc acgaatgacc gctttcatgc agtcaaatcg
     1681 tcccgagctt atttccaata ctgaatttag aatattagtt accaatgagt tacaagaaaa
     1741 ggaattaaaa cggctgaagc cgactatttt gaatttttta aagaccgctt taaaaaattc
     1801 aaatataaaa atgtctattc aaattcaaga agataggtcg gtacaaaaag gaagaactcc
     1861 tgaagagaaa tataaaataa tggcagaaga aaatccggct ttagcaaaac ttagaaatga
     1921 tttgcaactc gaaattgatt agcagccggc aaaaaaaatc tgaaatgtta aattgattca
     1981 ataaaccagg ttcgacataa aaatgcatta ttcagtacac caagaaaaaa attaaatttc
     2041 atcagaatat aatttatttt ttaatggtcc gatggaaccc acatgtctga atattatatt
     2101 cattcgtgtt tgtgtatgta atacatgtgg gtttcttaaa accagataaa tttttataac
     2161 gaaatatgta taataacagg ttgtacttgt atcttgaatt cagcaattaa taaataagca
     2221 ggtaaatttt tattgcctgc ttatttaatt ttttttttta tactttcgca catttttttc
     2281 tacgttattc ctttataatt ttgtgtgcgc ttttgccgtt tgccgtttcc agttcaacca
     2341 ggtaaaatcc ttttttcagc attgaaagat caatttcttt tatatcattg ttttgctgtt
     2401 taagcaacga accctgcaag ttataaatgc gaatgctttg gatatttgcg gtagaggaaa
     2461 tgaacaattt cccgcttgtc aaagttggat aaacataatc gctcacatta taggatattt
     2521 ctctgttgtc attagtaaaa tttacatttc ggtcagtgaa aatcagtagt tggccgggct
     2581 gcatgtttat agtcatgttg gtattgctca cattcagggt ttcgcctgtc agcaaattgt
     2641 accatgtgcc ggtgcgttga aaggacgggt tggctgttat tgaagcatca gattgaaaat
     2701 tacccaaaac taccatattc aggtcagcat gagagagagc tattcttctc ccctgcgacc
     2761 agtcgttata ggcgacttgc aaagaataat ttccgttcag aaacgcatta ggatacaatt
     2821 tacgcataga tataattttt gcacaggcgt cggcagccgc ttttcgatgc ggcaaattca
     2881 gccagcccca agcgtgaggt tttgggttgg ttcggcctcc aaacgaatcg atggaataat
     2941 cgtaacccat ttcttcaaat tgccacaaca tttttgggcc gggcatcaaa gttgcgaacg
     3001 ccactaccaa aggtacacgt gaaattctgt ataaagaatc agtcttaact gtgccattcc
     3061 cataggtttt ggccttatag aaatttctct cttcatcatg actttcagca tagcccaccc
     3121 atctgcgcgg agaagtattc atgccagaaa aatcactatt gctctgaaaa cccattgcag
     3181 cctgcgaata tgcgttattc aaatttcgcc acaaatacat accctgcgct gccagataat
     3241 cttcctcgct tcctcccaca aaatgttcga gaataaacat ggcatctggt ttcacgtcgg
     3301 ttactgcgta ataataatcc ttcagattgt tgatacgggt ttggtcccat gtcgattcag
     3361 tgcccgaatt ttgcgtaaat cctttggtca aatccatccg gtagccatct actttgtatt
     3421 cagtaatcca atactgcagc actcttttga aatattcttt ggtgccggaa taactatgat
     3481 tgaaatcgtt caatacactg aattgatgag gtgccacggg attcatccat ggattatcgg
     3541 cagcagggcg attttttgtg ctgtcccaat aaagcagtgc aaacggacaa attcccgaag
     3601 cctgattgaa aaccatatcg agaattacag ccatacctct tttgtggcat tcgtcgataa
     3661 attttttgta catttcgggt gttccgtaag ctttatccgg cgcaaaataa tgattaggat
     3721 tatatcccca actgttgttc ccatcaaatt cctgtatagg cattaattcc acagccgtaa
     3781 ttccgagatt tttcagataa tcgagtttgc cgatggcagc ttgaagcgat ttttctgtag
     3841 tgaaatcgcg cagcagcatt tcgtaaatca ccagattttc ttttgccggc atggaaaaat
     3901 tggtaatttc ccaattatag gcaggtttcg cagtttgaaa agttgcaacc agaccttcag
     3961 ttttaccttc gggataaggt ttcagatttg gataaatggt gtaatactga ttgatccatt
     4021 tgtcgttcca cgggtcgagc acgagttcgg tataaggatc gctgcagcga atcgttccat
     4081 caaccagata ttgataggcg taaatttttc ccggagtcag tccggttaag gtaatccacc
     4141 agtattcacc atcttttttc aattgatagg cattcagttg tgcccagtca ttaaattctc
     4201 caataagaaa aacattggtt ttattgggtg catgaaaaac caaagttaca gttgtgttgt
     4261 cgatgtaatt gattcccagt ttagtccctg ccggacgagt ttgattcaca accggttcgg
     4321 gaacgcaaac cagcagggta tcgtaaacgg tattgttgtc gactttggca gaagcaataa
     4381 aagtataatc gcctgccaca ctaaaattgt aggaataagt tagtgaggtg gctgcctgtt
     4441 gtgtagccag aacggtgttg ttgagcaaca atttaagttc ggcactcatc gaagaactaa
     4501 ctgtaaagtc gatactagag ccgatagcga cactctgatt ggaggttggg aaagtaaaag
     4561 ccacattaaa attgctttca taaacagtta caaagatatc cgctcctcct atatcttttc
     4621 cctgtagggt accagtgcta tttcgaaaaa caaaagccaa ctttctgaca acttctccgg
     4681 gacttaaatt ataatatcct gccatattgg gggttatcaa aagcttccat ttgttattgc
     4741 ccagcgaagt caacttatat tttggagaat tgtcacccca aacaggtgca tgtttccaat
     4801 cgctcggtga agtgcttgat gaggtgatta cgcctgtgtg agcatataca tcgccggtgt
     4861 aatctttcaa acctgcagtg ccttgtgtgg cgtcataaat aatttctatt tctcccgggt
     4921 agtctttggt gataaaagtg ggattggtag taatgatttg tccccagctt aataaatagc
     4981 aaagcagtaa tgaaaaagtg aaaagaattt ttttcatgat aatagcattt aaacagtttg
     5041 acttacatgc aaatgtaaaa gtttagtttc taaaacatcg aattctctat aaaaattaaa
     5101 agattttttc gtgcaaacga ttgcaaaaag aatacagtat ttttatttgc ctttattgta
     5161 aataactaaa gtttcgcagt tctttatttc ctgtctttca aatcattaaa gcgaagtgcg
     5221 aaactttagt aaataatctt tcgtaagtta atagtgtaca tccaagagca accgtttaac
     5281 attgccaatg gcaatgtaca gccagggtaa tatctttggc atgtcgtctt tcttcatgac
     5341 ctgtgcatga tgtgatttga cattttctgt caacttgttg taaggagtag catcataggt
     5401 gatccattca accattggat ttatgtgttc tttgacagtg tttaaaatta cgtcagcctt
     5461 ttaatttagg atattttgcg aattatcatt ttatattatt tcgaccgcgc tagcttaatg
     5521 tatctttata cactatttgt caaaagtcaa gatagcctta ttatcattaa attgaaaaag
     5581 tatcatattc tgtcaaaaaa tgatatgaat tatccactgg ataatgttat ttttgtcttc
     5641 actttataat attgagtagt gttactttaa aaaatctaat atcatgaaat tttctttttt
     5701 gtcatcactc tctttaatta tcgtttctgc caatatttgt ttgtctggtt ttgcacagtg
     5761 cgggaaaggc gaatatacaa tctcagcttt tccgtgttta aactgtgctg taacctgtca
     5821 aaaacctgca ggcccagaat ggatacacaa tgctacattt tatcaaattt atccacaatc
     5881 gtttaaagat acaaatggcg atggcattgg cgatttgaaa ggtgtaatca gtcagttgga
     5941 ttatgtaaaa agcttgggtg tagatgctat ctggattaat ccgttttatg tatcgccatt
     6001 cagagatgca ggttacgatg tcgccgatta ttaccatgtt gacccacgct acggtaccga
     6061 tcaggatgcc aaagatttat tccggattgc caaacaaaaa ggacttcgaa tcattgtgga
     6121 ttttgtgcca ggacatacat ccattgatca cccgtggttt aaggcctcgt gcagttcgac
     6181 gccaacaaaa tacagcaact ggtatatatg gacaaatgga acctggttcg atgacgaaaa
     6241 cggcaagtat aaagcgaatt ttattcaggg cttttgcgac agggatggca attacatgat
     6301 taactttttc tggcatcagc cagcattaaa ttatggttac ggtcagccag atccgaaaca
     6361 accctggcaa ttaagcacca ctcatcccga tgtcgttgca ctacgtaccg aaatgaaaaa
     6421 tgttatgcgt tattggcttg aaatgggctg tgatggattc agggttgata tggctggttc
     6481 gctggtaaaa aacgaccccg aaggaagtat taagtatttt tggcaggaca ttcgaaaaat
     6541 gcttgatcag gaatatcccg atgcattcat ggtgtcagag tggagtgaat cagctactgc
     6601 tgtcaatgcc ggttttcatg ccgattttat gctttggcag gaaggttatc aggacttgtt
     6661 tatgcacgaa aatagttatt tcaggtctgc gggaatcgga agtatcagtc attttatgtg
     6721 gcgatacaac gaacaaagca ctgaaacaaa tggcaaagga tatatttcgt taactgtagg
     6781 aaatcacgat ttggaaagaa taaataacag ggggcgcaac agggctgatt tggaaatcat
     6841 tcaggtgttt aactttacga tgcctaacat tccgtttatt tattacggag atgaaattac
     6901 catgcgtcaa ctgccgcttg ttacaggaaa agagggatgc tacggtacca gagcaggagc
     6961 tcgtacacca atgcaatggg ataccacagc gaatcttggc ttttcgacca gtgctcctga
     7021 aagactctat ttgccagtcg acagttcaag gaatgctcct aatgttttag cgcaagaaaa
     7081 tgacccggat tcatttttaa attttatgcg gaaattagtc ctgttacata aaactgaacc
     7141 agcattgaca aattatgcaa aatttgttcc cgtatatgtt caaaaaaaca aatatccttt
     7201 cattttcatg cggatatttg gtaacgaaaa attgttggta atacttaatc ccgatagtaa
     7261 accggcaaca gcaattgttg aaggaaacct gaaaggcaag cgaaaattat tgctcggcaa
     7321 acttccggtt tggaaacaaa atacaggaac aaccgaaata caggtagatg gacaatccta
     7381 ttcagttata aaactcaaat aagtcttttt attgatgaaa aaggtgagcc gatattattt
     7441 tattttctca gcgttagtcc ttttattgct gttttcaggt tgtcgtagtt tgcaaaaaaa
     7501 ctcaaaaacc agtaaaacag gcttcactcc tgaggcttat acggctttgt atgccgataa
     7561 gtggtcggca ataaacgaca gtgtgaaagc cggttggaaa aatcacagcg gaaaaccatc
     7621 taacaatgct gtgcccgaca catttgcgta tgcatttaac gagtggacat tattttattg
     7681 ggatttgtat tttacacaaa tcggattatt gaaacataac gaactaaagc tagctaaagg
     7741 agggataaat aacctgaaag cggaactgga caagcgtgga tttataccca atgcgaatac
     7801 ggattggggg gaaaaccgtt cgcaaccacc ctattttggc ataatggtaa aagaatttta
     7861 cgattttaca aaagataaaa aatggttaaa aacgatgtat gctgccgttt tgaaagaata
     7921 caaattctgg acagatacat cggcgaaccg aatagaggca aatacaactg aacttggtct
     7981 tcagcgctat tttcaccatg ccaccgaaac agagctactt gaactgtatg atcaggaact
     8041 cacttctcgt tttgactttc caaaagatat tgaaaaatcc aaaaaattga atattgcttc
     8101 gcaatttgct gctgaagctg aatcagggat ggactttacg ccccgtttcg aaaatcgctg
     8161 ttcggagttt atagctgtcg atttgaactc caatctatat ttgtacgaaa agtatctggc
     8221 ttattttgaa caagaactta aaataggtaa tggtcagcaa tggttagcaa aagcccgaaa
     8281 aagaaaagcg cttattcaga agtactgttg gaacgatgaa cgtgggttat ttctggatta
     8341 tgattttgta aacaacagaa gcagtaaagt agcagctgca acagcatttt caccgctctt
     8401 tgcaggtctg gcctcgaaat atcaggcaga ccgaatgcgt gaaaatctgc atctgttcga
     8461 gagtgaaaac ggaattgtag cctgcgaaag cacggatgaa aagagaattt accaatggga
     8521 tcatatttcc gtctggtcgc caatgcaatc attggttatc atggcgcttg ataattatgg
     8581 ttttaaggaa gatgcctgcc gcattgccat gaaacatcta gacctgataa cagccaattt
     8641 ttttaaccca aatccatcaa cttatgtaga taaaagcgga aaaaaatttt acagattgcc
     8701 aggaggaatt tatgagaaat acacccgatt tggcacaata aacgacaggg aatataatgc
     8761 aagtctaatg tatggttggt cggcaggagc ttttgctttt gcatacgatt atttaattgt
     8821 taatagtcaa cataatttta ctataaaatg aagaaaatca gaattttatt tataattgct
     8881 tttttgttca tttcattgtt tggacaaaaa attttatgtc agtctgaatt aaaaataact
     8941 tcggaccatc attatttgag ttataaaaac ggagacccat ttttctggct tggagataca
     9001 gcttgggaat tatttcatcg tttaaaactg gaagagattg aaacatattt ggaaaacaga
     9061 aaacaaaaag gatttaatgt tgttcaagca gttatacttc ctgaaatgga tggattgaga
     9121 gttccaaaca gatacggcga tgttcccctt atagatatgg atcctacaaa acctaatgaa
     9181 aaatattttc agttcgtcga tacggttgta aaattagcag caaaaaaagg tatttttatg
     9241 ggtttgctac ccacttgggg agataaagtt actcaactct ggggtgaagg tccggtggtt
     9301 ttcaatcctg aaaatgccta taaatatgga aaatggatag gcgaaaggta taagagttat
     9361 aaaaatatta tatggatact tggtggcgac cgacctccca tgaaggatag tgccgactgg
     9421 cgacctgttt ggagaaatat ggccaaagga atagaaaaag ctaccaatca ttcagcactt
     9481 atttcttatc atatctgggg aggtgaaatg tcaacatcac aatatattca ttccgaaaaa
     9541 tggctggata taaatatgat gcagtcgggt catggttcgg gaaaagatgt tcctgtgtgg
     9601 gaatggataa aacgggatta ttgtatgaag ccaacaaagc ccactctaga ttcggaacca
     9661 aattacgaag atcatcctgt gaatccgtgg ccaagttgga atcctgaaaa tggttatttc
     9721 agagattacg atgttcgcaa gcagacctat cgttctgttt ttgcgggagc atgcggtgtt
     9781 acatacggca atcacagtat ctggcagttc aggaatgaac gcgacccaaa tataaatttt
     9841 gcggagagat actggactga agcattggac agacccggtg catttcaggt tggatatttg
     9901 aaaaaactta ttgaatcacg accatcatca agcaggattc cggatcaatc cattattctg
     9961 gaaggtcagg gagaaaaaga agagtatatt tgtgctttca gaaatcgatc aaatgattat
    10021 gccatgattt atattcctgt tggtaaaaca attacaatca aaacatcatt tattaaaact
    10081 aaaaaactga aaatcagttg gtttaatcca caaacatgtg agacaattca aagcatagaa
    10141 acaattaata ccggtaaaat gtgtgttaca actccaacaa ccggttttgg taaagattgg
    10201 gttttgatag tagaaaaaga tttagtaaat acagcaaagt aagattttat ttttatttct
    10261 aagtacatga caaaaatttt aaaaatatca gaatttgatt taaaaccccc atttaaagca
    10321 ctattgcaat gaagttcata tttgaaataa ctttttccca ttctaccatt gttcagcatt
    10381 gtgattggct attcatgttc gtgtaaaaaa aatttcgacc agatatgccc aacaaaatgc
    10441 aatggttact atagcgacaa gttttttaat cctataaata accctcaaaa gagtgtcttc
    10501 taaatttaat ccagttgttt tcattataac cgattgttag tcagctataa agaaaaattt
    10561 caaggtcttt accaaaaaat gacaattatt ttttgattgt caagtaatta tatcgcaata
    10621 taattattgt aatatacgga atattatttt ataagtcaag catttgtgaa tcgatagata
    10681 atttgaaccc agttctgaat gaaaaaaaac acattattat tactgcttct aatatatttt
    10741 gagatgctta cagctcaaaa tcctatttgc cctcccggtt tgaatattgc cgacccttct
    10801 gcacgcgtct ggaatgatgg gcggctatat gtgtacggtt cacgcgacga aagttttaat
    10861 tattattgtt cttttgatta ctgggttttg tccactgatg atttaattca ctggcaatat
    10921 acaccgaatg tttttgcgtc taaaggacaa tacgatcagg tgccatttaa cgataatttg
    10981 ttgtttgcac ctgattgtat gtataaaaat ggcacttatt atttgtatta ttgccagccg
    11041 gatggcattc aaggagttgc taccagcctt tcaccaatcg gtccgtttac aaatgctaaa
    11101 gaaatttatc tgaaaaataa gaatcaaata gatcctgctg tttttgtgga tgacgatggt
    11161 caagcgtatt atatgtgggg tcaatttcag ggaaaaattg ccaaactaaa acctaacatg
    11221 acagagattg ataccactac cattgttgat aatctggtta ctgaaaaaga gcattttttc
    11281 cacgaaggcg gctatatggt caaaagaaac ggaatctact attttgttta tgcccatgtc
    11341 gggcggaaaa atatgcctac ctgcattggt tattccactt caaaatctcc tttgggtcca
    11401 tttaaatacg gaggagttat tatcgacaac gatggttgtg atcctgctaa ctggaacaat
    11461 catggatcaa ttgtcgaatt taaaggtcaa tggtatgttt tttatcatag aactaccaac
    11521 ggatgtgtca attcacggaa agcctgtatt gaaccgattt atttcaacac ggatggaagt
    11581 ataaccgaag tagaaatgac ttcgcagggc gcaggaaaac cgcttgatgc tttttctaaa
    11641 atccaagcag agcatgcttg tctgctttcg ggtcatgtcc gggtagaatc ttattccttc
    11701 gacacaaata atcctttaaa tccgactaac agcgatcaac tggggcaaat tgaagataaa
    11761 gaccgtgcta tgtataaata cattgatttc ggagaaggag tcgattccgt ctcaatacgt
    11821 attgcacccg gattaaattc cggaaaaatt agctttatac tggataattc atggtcatta
    11881 ccttttgcag agatagctgt ggacggggga ggagatggta aaacatggaa aatttttagc
    11941 tgtaaagtta agcgtgttaa tggtattcat gctctgaatc tcacatttag ttcaactgga
    12001 agcaatactt ttaatataga ttggtttaca tttaaacatc aataattcaa cttaataacc
    12061 aatattatat ttatttcaaa gcttaaataa aaaaaataaa aaaggtattt cttttgtatc
    12121 atggtcattt tattttcatt ttttgaactt aaataacaca gttcttgtaa taataattaa
    12181 cactttaaat taatgaaaaa attagtattg cttgcattta ttttttccac aatttttgct
    12241 ttcggtcagt taccaacatt taaaattagt aaaaaagtca tcgctgatat tgatgataaa
    12301 ctcttcggcc aatttatgga aaaacccagc tgggacggcg aaataggagg cgatggtgca
    12361 attgataaaa aaacaggttc tgtttcaccg gatgtattgg cttatctcaa ggaaatgaaa
    12421 attccgatta tacgctatcc gggaggtacc gatgttgatt attatcagtg gtataattta
    12481 atagaaaatg taccgggaaa acaccagaag cgcccggctt accgaaatta ttctgggaca
    12541 ggcaagataa cttccgataa tcgccttggg ttggatgcgt ttttaactct ttgttcagag
    12601 cttaaatccg aacctctttt ggtggtcaac atcggagatg catttttcag gaaaatcagt
    12661 attgaggaat cggcaaaaaa tgcagccgga tttgtagcat attgcaatag caaggtggga
    12721 acaaaagttt cggaagggat gccgcctgat tggccagcaa tcagggcaaa gaacggtcac
    12781 acgaagcctt acaatgttcg ttatttcgaa attgggaatg agattcaatt atttaagtcg
    12841 atgaaagaaa aagatgtgaa cgatagtttg gtaaatcatt atgtcaattg cttggaggca
    12901 attgtgaatg cgatgcttga cgttgaccca aatatcaaga ttattaccga tggagaaata
    12961 aaagaagttt ctgaacaaat aaaacgcaga ctgggcaata aaatcagtta tttagcatgt
    13021 cattattatt taccatggga aattaatacg gtaaaattgg ataccactac aatagatata
    13081 aaatcattaa cccaagctga tatttggaat gcatgggtgg caacgcctta tattcatccg
    13141 gaaacaggac aatcttattt tcccaaaaca ggcaaatata atcatgcagc cgatgccgga
    13201 tacgatttgg ctataacaga atggaactgg aatggttggt ggaacaaaaa gatcttacca
    13261 gatgcagcat taaattcgct ttttgcaaaa ggaataggtg cagctggttt tatacatgca
    13321 atgatgcgtg atgccggacg gatgaaaatt ggttgtcagt cgatgttggt tggcaacgta
    13381 tgggatatta atgccattcg ggtggacact accggaaaga tgaaggctcg atttttccca
    13441 acgggtaaag ttaccacact ttactcaaaa tatcacggaa accaattatt ggagatgagt
    13501 gaaaagaatc agttgtttta cacacagcca tataaaatga actcaatcga agctgtatcc
    13561 cgggtgacat atgtcgatgc attagcatcg cgcaacgaaa ctacgcttta catatcgttc
    13621 atcaatcgaa attttgcgaa tgatatggac gtggaaattg atttatctga ttttaaaact
    13681 gattcaaaat ataaacagca catttttaca acaaacgata tgataacaaa cgaattgggt
    13741 tattttgaag ataagtctga aaaagttaaa aaccataagc cgctgattgt tcatcttcca
    13801 aaacactcat tatcgatttt tgagattaaa attttttaga aataagttct ttgcgtgaaa
    13861 aaattaaaag agggtgttcc aaaaggacac tttctttttt agattaagca gctgatttaa
    13921 cccaactttc cattcattag ccggaactca ttgctaaaca agtaaatata ctgtctggag
    13981 gatgagtttg ggtactacaa atttttcgtc gtttgaatag ctgttgtttg tactttaacg
    14041 ctt
//
LOCUS       DAOSIB010000059        13836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_4518_length_13836_cov_11.825557, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000059 DAOSIB010000000
VERSION     DAOSIB010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13836
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4518_length_13836_cov_11.825557"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(335..3109)
                     /locus_tag="PLD44_06290"
     CDS             complement(335..3109)
                     /locus_tag="PLD44_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOF98977.1"
                     /translation="MRKIFFFLSLFFFPILIQAQTFSGSALITGKLIDKETKKPVPLA
                     NIRVLQQRDSSFVNGQASRNDGSFSIPVHYGSYIVHISFLGYDDVFKHVQVSASNPIA
                     VLDTIFLRQTDFKLSEVIITEKAPEVRVKGDTLEFNAGSFKVTESAVVEDLLKKMPGV
                     EIDKDGKITVNGREIKKIMVDGKEFFSSDPKVASKNLPAKMVEKVQVLDKKSEMAEMT
                     GFDDSDEETVINLVIKQGMKEGLIGNAFAGYGSKDRYEGNAMVNYMRNQNQFTFIGGL
                     NNTNNAGFTDLASSMFGNMGGRGMPGMFGNQDGVTTSGMGGFNFNTQLSQTFKLGGNV
                     RYGSTNSDVLSKIRTQNMLSIGNTFENENDTTNSFSQNFNMDLRMEWTPDTVTRIIFR
                     PSVGVYENSRDEISNFNTTNDENDSINKGDSKYSSDGNGYNVGGRLEVSRQLGKKGRV
                     LSLQLRGQLNDADNDGYNLSNTYYFGTKPDDIIDQKFTNTSNSKNWQSFLSYVEPIGH
                     NNFIQLAYNYQQKFSESNKDTRTKDDLGNYSVMDTTYSKRLENDFVNQSIEMNFKANR
                     GKYDYMFGFSMQPFNSSSKTYKGDSLYNNTSNQGINYAPSAQFNYRWSRQNNLRIRYS
                     GITSQPSVTQLSPVRDVSNPLNVTYGNPDLKPSFSHQLNIRYMNFMPQQKSSINFFGN
                     ASYTLNDIVSSTSTDVFTGQKQTTYKNVNGNWNANGRLTLNLPIKKTNFSVFSMSSGS
                     YTHSKGYSNNEINLSQRTGLGQTLGIDFRSGVVDFGTRGNINYNNIQNSLTGQQDQEY
                     FNYNVSANTTIYLPYDLSIDSDLSYNTNSGYSTGFEQEALIWNASLQKQLFKHKNGSL
                     RFKIYDILQQKSNISRRATANYITDTTTNTLTSYFMFHFVYRFNIFKNGANQNDMMPR
                     RSFGGGPSGH"
     gene            3548..5422
                     /locus_tag="PLD44_06295"
     CDS             3548..5422
                     /locus_tag="PLD44_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF98978.1"
                     /translation="MKIKQTKNISPWAWVPTLYFAEGIPYVVAMTVAVIMYKRLGISN
                     ADIALYTSWLYLPWVIKPFWSPFVDLIKTKRWWITSMQLLIGAGFAGVAFLIPAPFFF
                     QATLAVLWLIAFSSATHDIAADGFYMLALDSSQQSFFVGIRSTFYRFAMIAGQGILII
                     IAGALESHTGMEPVVFSVQSSPDPISQIVNVDSLSKVNFPEDAEMKFVVFPASLSLST
                     QPITSDSLNKIKAAAVENNKRYRFIAEENLQNDQTKKENDSWWHQHVSGPFADFIREN
                     FGEKREKISMVENLTGNAGVVAIRLTKKPEAGKPLILNLSQSKGDKSISLVSGERLVF
                     TDENWDKPALVVVQLDAKLKGQAISEFKGVSGNIRLAWSITFFILSGLFILFYLYHRF
                     ILPRPASDHPSLTHSSKDIIREFGRTFATFFQKKGIGLAIAFMLLYRLGEAMLVKMSS
                     PFLLDPHEVGGLGLTTGQVGLVYGTVGVIALTLGGIVGGIAASRKGLKYWIWPMALCI
                     TLPNMAYLYLSWFMPENMLLINAAVAFEQFGYGFGFTAYMLYMIYFSEGEHKTAHYAI
                     CTGFMALGMMLPGMMAGWLQELIGYNHFFIFVLICAIPTLAIIPFMKIDKEFGKKKSV
                     "
     gene            5602..6375
                     /locus_tag="PLD44_06300"
     CDS             5602..6375
                     /locus_tag="PLD44_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOF98979.1"
                     /translation="MKILILGAGKMGTFFTDLLCLHHEVALYDVDPQKLRFVFNTYRL
                     TQYKEIQEFEPELVINCVTLKYTIEAFKNVLPYLPENCMLSDIASVKTGLKEFYESTG
                     KRFVSTHPMFGPTFATLDNLSSQNAIIISESDYLGKMFFKDLYHSLHLKIFEYSFEEH
                     DKTTAYSLAIPFASTLVFAAVMKHQDAPGTTFKRHMDIAEGLLSEDDYLLTEILFNPY
                     AEEQIDNIRAQLKVLLDIIKKKDAARMQEFLEKVRKNIE"
     gene            6434..6610
                     /locus_tag="PLD44_06305"
     CDS             6434..6610
                     /locus_tag="PLD44_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOF98980.1"
                     /translation="MNTKEIVLEAMKKAGSPVNAGKIVEMTGLDRKEVDKAMNELKAE
                     GAIESPKRCYWQPK"
     gene            6914..8035
                     /locus_tag="PLD44_06310"
     CDS             6914..8035
                     /locus_tag="PLD44_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HOF98981.1"
                     /translation="MPSIVIDSYIPYLRGVLDEVMDVTYLPHSQITNETVKNADALII
                     RTRTKCDARLLSGSKVKFIASATIGYDHIDAAYCAENGIKWTNAPGCNALSVAQYMAS
                     VLSYVNRNFFELAGKTLGVVGVGAVGSKIVQLGASFGMNVLQNDPPRARKEDPYQFVS
                     LSEICSTSDIISFHTPLNMEGEDKTFHLADKKFFDTLKKKPLIINAARGEVISTSALL
                     DALNRQGILTAVIDCWENEPDIDTELLNKTALGTPHIAGYSAEGKANAASQSVRAVSK
                     FFGLGLDNWEVSNLPEPTKVDLSFVNNIEDFFLKTYNIESESNQLKNHPRLFEYLRSH
                     YPFRREPDAYLPQLNEIMQKQLIEQFPLFFNQKNFKTLA"
     assembly_gap    8173..8272
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8273..8898)
                     /locus_tag="PLD44_06315"
     CDS             complement(<8273..8898)
                     /locus_tag="PLD44_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HOF98982.1"
                     /translation="MKNIEAKNSILCRYLIANEQDTRWGLTITTVGKQSLSINEDYPP
                     KDGHPMGYLFSTEQGRVLDEYQLLYIVKGSGIFTSTSCKLTNINAGNMFMLFPGEWHN
                     YKSNPATGWEEYWIGFKGENIDKRVGNGFFCKENPVYKVGVLDEAVQLYNQGILVAIE
                     QKSGFQQMLAGIVNHLLGLAFCLNKYNEFENLEITNLINEAKIRFMED"
     gene            9212..10690
                     /locus_tag="PLD44_06320"
     CDS             9212..10690
                     /locus_tag="PLD44_06320"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HOF98983.1"
                     /translation="MSKLPHFFAVDLGATSGRTILGTLQNNRLDLKELTRFQNKIICI
                     HDKYYWNIYSLFEEIKKGLLIASQQGIHINAMGIDTWGVDFVYLANDGSLLNLPRSYR
                     DPYTNGVPEEYFSRISKREVYELTGIQIMNLNSLFQLYAAKKESSSALEYADSILFMP
                     DALNYLFTKKKVCEYTIASTSQLLNPFTKKIEAELLVPMNIEPTILPQMVMPGTVVGN
                     LEKSIQRECNVPEIPFIAVAEHDTASAVVAVPAEDENFAYLSSGTWSLMGIETKNPII
                     TEESYRLNFTNEGGVDGTIRFLKNITGMWLLERCREEWKLDGNSYTYPEIVQMAESVR
                     SFQFLIDPDDSVFSNPESMTRSIAVFCRNSGQKVPATHAEFIRCIFDSLALKYKYVLD
                     SLQKLSPFPINRLHVIGGGSQNNLLNQLTANAIGLPVIAGPAEATAMGNIMMQAKGLG
                     LINSLTEIRSIIRNSVELETFYPQNTELWEKAYKKYLHILEK"
     gene            10724..11983
                     /locus_tag="PLD44_06325"
     CDS             10724..11983
                     /locus_tag="PLD44_06325"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOF98984.1"
                     /translation="MKKDEIIQKSYEIAKERYEAVEVSVDKTIEVLEKLSLSIHCWQA
                     DDVTGFENFGNINDAGIQATGNYPGKARNMDELRQDIEKVLALVGGKHRLNLHAIYGD
                     FQRKKIDRNEIEPSHFTSWMQWAQEKGLKLDFNSTSFGHPKSGNLTLANPDKEIRNFW
                     IEHTKRCRLIAEEMGKFQHDPCIMNLWIHDGMKDLTVNRLLYRSILEKSLDEIFAKGY
                     TNMKDSIEAKLFGIGLESYTVGSYDFYLAYGTKNQKIVTLDTGHFHLTESVADKISSL
                     LLFVPEIMLHVSRPVRWDSDHVTILNDDVTELAKEIVRADALSRVHVGLDYFDASINR
                     IGAYVVGIRATQKAFFQALLEPVSLLRKYEDNGQYFERLALLEETKNLPWNAVWDYFC
                     LKNNVPVGEDYIAEIQQYEKNVTSKRN"
     gene            12120..13154
                     /gene="rhaT"
                     /locus_tag="PLD44_06330"
     CDS             12120..13154
                     /gene="rhaT"
                     /locus_tag="PLD44_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HOF98985.1"
                     /translation="MNTILGLIIIAIGSIGQSSFYVPIKKVKNWSWESFWLMQGIFAW
                     LVFPFIGSLMAIPAGHSLGEIWSAGGSDMWKSVGYGILWGIGGLTFGLSMRFLGIALG
                     QSLALGTCSAFGTLIPALLKGDNLLKGDGLILLIGVCLAIAGIAVIGYAGSLKAKNLT
                     EEERKKAVKDFALKKGILIALLAGIMSACFNLGLESGSSIKTALLNIGGNSLMVLNPV
                     IFLVTIGGFLTNAVYCFYQNYKNQTFSDYTRSSGKIWMNNLLFCALAAVLWYSQFFGL
                     GAGQSFFAPGSVMMAFSWSILMSLNVLFSNIWGIILKEWNGVGKKTIFVLIIGLAILI
                     FSIIFPQIVK"
     gene            13743..>13836
                     /locus_tag="PLD44_06335"
     CDS             13743..>13836
                     /locus_tag="PLD44_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98986.1"
                     /translation="MPKQGQSVESCIVTEIKKKKGDTVKKGDILF"
BASE COUNT         4161 a         2297 c         2648 g         4630 t
ORIGIN      
        1 ataacaaaaa aaaatagaaa aattatttca gtatataaaa aattttaatt ttttcagatt
       61 tttatattga aaaataatag tataaatcat tgtttatgag tttgttacaa aaaaaaataa
      121 tttttattca tgcaatacat catgttcaag ggttatattt tgttcggaat tgctcttgta
      181 aatcttttgg aaaaagcaaa gaaaaagaat ataaaaacct taatctgtta taacaactga
      241 atttttatta caaaatttgt cctataaaca gttgcattta tgattttaat acagtagtta
      301 ttgttctgtt ttttcaagta ttgtcagcat agggtcagtg tccactgggt cctccaccaa
      361 agcttctgcg cggcatcatg tcgttttgat ttgcgccgtt tttgaaaata ttgaaacgat
      421 acacaaaatg aaacataaaa taggaagtaa gcgtgttggt agtagtgtct gtaatataat
      481 tggctgttgc tcttctgctg atgttgcttt tctgttgcag aatgtcgtaa attttaaagc
      541 gcagggagcc atttttgtgt ttaaaaagct gcttttgaag ggaagcattc catatcagtg
      601 cttcctgctc gaatcccgtt gaatagccgg aattggtatt gtaagatagg tcgctgtcga
      661 tcgataagtc ataaggtaaa taaatagtgg tattggctga aacattgtaa ttgaaatatt
      721 cctgatcctg ctgacctgtc aaactgtttt gaatattgtt gtaattgatg tttccccttg
      781 tgccgaaatc cacgacaccc gaacgaaaat caattcccag tgtttgtccc aaaccggttc
      841 gctgacttaa gttaatttca ttattggaat atcctttgga atgagtatag ctgcctgatg
      901 acatggaaaa aacggaaaaa ttcgtttttt ttattggcaa attcagcgtc agtcgtccgt
      961 tggcattcca gttgccatta acatttttat aagtggtttg tttttgtccc gtgaaaacgt
     1021 ctgtagaagt ggaagaaaca atatcgttca acgtgtagga agcgtttcca aaaaaattga
     1081 ttgaactttt ttgttgcggc ataaaattca tatagcgaat attcaattga tgggagaaag
     1141 aaggttttaa atcgggatta ccataagtaa cgtttaaagg gttggaaaca tctctcaccg
     1201 gtgaaagttg cgttacggaa ggttggcttg taatgcccga atacctgatt cttaaattgt
     1261 tttgtcgact ccatcgataa ttgaattggg cacttggtgc ataattgata ccctgattgg
     1321 aagtattgtt ataaagagaa tctcctttat aggttttact gctggaattg aacggctgca
     1381 tactgaagcc aaacatatag tcatattttc ctctgtttgc tttgaaattc atttctatcg
     1441 attggttaac gaagtcgttt tccaatcttt tgctgtaggt agtatccatt accgaataat
     1501 ttcccaagtc gtctttggtt cgtgtatcct tgttcgattc ggaaaatttt tgctgataat
     1561 tgtaggcaag ctgaataaag ttattgtgtc cgatgggttc tacgtaggat agaaaacttt
     1621 gccagttttt gctgttgctg gtattggtaa atttctgatc aataatatca tcaggtttgg
     1681 taccaaaata ataagtattg gatagattgt aaccgtcgtt gtcggcatca ttcaactgtc
     1741 ctctcagttg gaggctgaga acacgtcctt tttttcccaa ttgcctgctt acttccaagc
     1801 gccctccgac attgtatcca ttgccatccg aagaatactt tgaatcacct ttatttatag
     1861 aatcattttc atcattggtt gtattgaaat tactaatttc gtcgcgactg ttttcataca
     1921 ccccgacgct tgggcggaat atgatgcggg taactgtgtc aggagtccat tccattcgta
     1981 aatccatatt aaaattttga ctgaaactgt tcgtggtgtc attttcgttt tcaaaagtat
     2041 ttccgatgct cagcatgttt tgtgtccgga tttttgacaa cacatcactg tttgtggaac
     2101 cataacgaac atttccaccc aatttgaaag tttgagaaag ttgggtgttg aaattgaatc
     2161 ctcccatgcc cgaagtagta actccatctt ggtttccaaa cattcctggc ataccgcgac
     2221 cgcccatgtt accaaacata cttgatgcca aatcggtaaa tcctgcatta ttggtattgt
     2281 ttaggccgcc tatgaaggta aactgatttt ggtttctcat gtaattgacc atagcattgc
     2341 cttcgtagcg gtctttactt ccatatccgg caaaggcatt tcctatcaat ccttctttca
     2401 tcccttgctt aataaccaga tttatcacag tttcctcatc gctgtcgtca aagccggtca
     2461 tttcagccat ttcggatttt ttatccagca cttgaacttt ttctaccatt ttggcaggta
     2521 gatttttgga cgcaactttg ggatcgctgc tgaaaaactc ttttccgtcc accataattt
     2581 tttttatttc gcgtccgttg acagtaattt tcccgtcttt atcaatttcc acaccgggca
     2641 tttttttaag taagtcttct accacagcac tttcagtaac tttaaacgaa ccggcattaa
     2701 attccagtgt gtctcctttc actctaactt caggcgcttt ctcggtaatt ataacttcag
     2761 aaagcttaaa atcggtctgt cgcaaaaata tagtatcaag cacagcaata ggatttgaag
     2821 ctgatacttg aacgtgctta aaaacatcat cataacctaa aaatgagata tgtacaatgt
     2881 aagatccata atgaaccgga atggaaaaag atccatcatt cctgctcgcc tgtccgttta
     2941 caaagctgct gtcgcgctgc tgtaaaaccc ggatatttgc taatggtaca ggtttttttg
     3001 tttctttatc aatcagttta cctgtaatta aagcacttcc agaaaaagtt tgcgcttgaa
     3061 tcaggatggg gaaaaaaaat aagctaagaa aaaaaaatat ttttctcatt actctgtctg
     3121 tttacgatat tttttgtttc aaaattatac ctatttaaat taatttccaa aaaagagaaa
     3181 atatttccct tgaaaaattg aagggaaatt cagcatataa atgtaagaat tgttggtaga
     3241 tgatttatca ttttttcttt taaattcaca gaagattgtt tacctttgcc tttcttaatt
     3301 tgaaagcaat tgtttttcag ttcgattttt tctgtcgtgg tttgtatttt tctaattctt
     3361 aagcaaagaa gggattttcc catatattgt gttacaattt tattgagtta gaaatatttt
     3421 aaactcgttt ccaaaattta tttataaccc tcaccctgac tttaattaaa gttgctatga
     3481 aaagtttata tgtttgaaat gattagaaac agaatttata acctctaaat ttttccgata
     3541 tatcgacatg aaaatcaagc aaacaaaaaa catttctccc tgggcatggg tacccacttt
     3601 atactttgct gaaggcattc cttatgtagt tgccatgacg gtagctgtga tcatgtacaa
     3661 gcggctgggt atttcaaatg ccgatattgc cttgtatacc agctggttgt atttgccttg
     3721 ggtaataaag ccattttgga gtccatttgt agatttaatt aaaaccaagc gttggtggat
     3781 tacttccatg cagttgctga ttggtgccgg ttttgctgga gtagcttttt taattcccgc
     3841 tcctttcttt tttcaggcta ctttggctgt actgtggttg attgctttca gttctgcaac
     3901 tcacgacatt gcggccgatg gcttctatat gctggcgctc gatagctcac aacagtcttt
     3961 ttttgtggga attcgcagta ccttttatcg ttttgccatg attgcaggtc agggaattct
     4021 cattattatt gcgggagctt tggaaagcca taccggcatg gaaccggttg ttttttccgt
     4081 tcaatcttct ccggatccaa tttctcaaat agtaaatgta gacagtctgt caaaagtaaa
     4141 ttttcccgaa gatgccgaga tgaaatttgt agttttccct gccagcttga gtttgagtac
     4201 gcaacctatt accagcgatt cgctcaataa aatcaaagcc gctgcagtgg agaacaacaa
     4261 acgctatcga tttattgcgg aagaaaacct tcaaaatgat cagaccaaaa aagaaaacga
     4321 ttcttggtgg catcaacatg tttcagggcc ttttgccgat tttatcaggg aaaattttgg
     4381 agaaaaacgg gaaaagattt caatggtaga gaatttaacg ggaaatgccg gtgttgtagc
     4441 catacgtttg acaaaaaaac cagaagccgg taaaccgttg attttgaatt taagtcagtc
     4501 caaaggcgat aaaagtattt ctcttgtcag tggcgaacgt ttggttttta ccgatgaaaa
     4561 ttgggataaa cccgcgctgg tagttgttca gctcgatgct aagctgaagg gacaggcgat
     4621 aagcgaattt aagggagttt caggaaatat cagattagct tggagcataa ctttctttat
     4681 tttgtccggt ttatttatct tattttatct gtatcacagg tttattttac cccgaccggc
     4741 ttccgatcac ccatctttga ctcattcgtc aaaagatatt attagagaat ttggacgaac
     4801 ttttgccaca tttttccaaa agaaaggaat cgggttggct attgcattca tgcttcttta
     4861 tcggttgggg gaagcaatgc tggtgaaaat gtcttctcct tttttactcg atccgcatga
     4921 agttggaggt ttgggactaa ctactggaca agttgggctg gtttatggaa cggttggcgt
     4981 cattgccttg actttgggag gaattgttgg cgggattgca gcttcgcgca aaggcttgaa
     5041 atattggatt tggccgatgg ccttgtgcat cacgctgcca aacatggctt atttgtattt
     5101 gtcgtggttc atgcccgaga atatgttgct tatcaatgcg gcagtcgctt tcgaacagtt
     5161 tggttatgga ttcggattca cggcttatat gctgtatatg atttattttt cggaaggaga
     5221 acacaaaact gcccattatg ccatttgtac gggcttcatg gcgctgggaa tgatgttgcc
     5281 cggaatgatg gccggttggc tgcaagaatt gatagggtac aatcatttct ttattttcgt
     5341 gttgatttgt gccattccga ctcttgccat tattccgttt atgaagatag acaaggaatt
     5401 cgggaaaaag aaatccgtgt aagaaatctt tctacttatt tgtaaaatga atcgttttgt
     5461 tttcattttt gccacttttg tattcgaaat tgaaaaataa caatttatga aacccacatg
     5521 tatcccatac acaaacacga atgaatataa tattcagaca tgtaggttcc atcagacctt
     5581 taaaaaataa attatattct gatgaaaata ttgattttag gagcagggaa gatgggcact
     5641 tttttcaccg atttattgtg cctccatcac gaagtggctc tttatgacgt tgacccacaa
     5701 aagttgcgtt ttgtttttaa tacttatcgg ctgactcaat acaaagaaat acaagagttt
     5761 gaacctgaat tggtaatcaa ttgtgttacg cttaagtata ccatagaggc atttaaaaat
     5821 gtgttgccct atttgcccga aaactgcatg ttgtccgata ttgcatcggt taaaacgggt
     5881 ttgaaggaat tttacgaaag taccggaaaa cgttttgttt ccactcaccc catgtttgga
     5941 ccaacatttg caacgctcga caatttaagt tctcagaatg ccatcattat ttcggagtcc
     6001 gattatcttg gaaaaatgtt tttcaaagat ctgtaccatt ctctacattt aaaaatcttt
     6061 gaatactctt ttgaagaaca cgataaaaca acagcatatt cgctggctat accatttgca
     6121 tccacattgg tttttgcagc tgtcatgaaa catcaggatg cacctggcac cactttcaaa
     6181 aggcacatgg acattgcaga agggctttta tcggaagacg attatctgtt gactgaaatt
     6241 cttttcaatc cctatgcgga agaacaaatc gacaacattc gagctcaact gaaagtgttg
     6301 ctggacatca ttaaaaagaa ggacgcggca cgaatgcagg aatttttgga aaaagtcaga
     6361 aaaaatatcg aataagcttt gtattgaaaa cattttcggg aaatgattgt tgtaaacaaa
     6421 aaaattaaca gctatgaata ctaaagaaat tgtactggaa gccatgaaga aggccggttc
     6481 gccggtgaat gcaggaaaaa ttgtcgaaat gaccggtctc gacagaaagg aagtagacaa
     6541 agccatgaac gagctgaaag ctgaaggagc catagaatca cccaaaagat gttattggca
     6601 accaaagtaa ttagaaaagt ttaggcaaaa aagggttcaa gggttatttc aaaaaatttt
     6661 tcttgaactg attactaata ataaagcgtc tgagaattta ttgattttta aggcgcttta
     6721 tcgtttaatc aatgtttata aagcctgttt ttacgcccag gatagtaaaa ttattgtcga
     6781 aaaataactt tttgaaacat tttatttgat ttctcacttg cctgttggct ttacaacttt
     6841 gatttttatt ttcacaagct tggaaataat tcaattttca tgtaattttg cagttcaatt
     6901 atttcaatca aaagtgccaa gtattgtcat tgacagttat attccttacc ttcggggtgt
     6961 attggatgag gtgatggatg ttacatatct gccccattct caaattacga atgaaaccgt
     7021 aaaaaatgca gatgccctta taatccgtac gcgtaccaaa tgcgatgcac gtttgttgtc
     7081 gggttctaaa gttaagttta ttgcctcagc cactattggt tacgatcata tagatgctgc
     7141 atattgtgcc gaaaacggta taaagtggac caatgcgccc ggttgcaatg cgctttcggt
     7201 tgctcagtat atggcttcgg tgctcagtta tgtaaataga aatttttttg agctggcagg
     7261 aaaaacttta ggagtggtcg gggtaggagc tgtaggttca aaaattgttc aattgggagc
     7321 ttcttttggt atgaatgtgt tgcaaaacga tcctcctcgc gccagaaaag aagaccctta
     7381 ccaattcgtt tctttatcgg aaatttgttc gacatccgat attatctcgt ttcacacacc
     7441 cctcaatatg gaaggtgaag ataaaacttt tcatttagcc gataaaaaat ttttcgatac
     7501 attaaaaaaa aaaccgttga ttattaatgc tgctcgagga gaagtaattt ctacaagtgc
     7561 attgttggat gctttgaaca gacaaggaat attgactgct gttattgatt gttgggaaaa
     7621 tgagcctgat attgataccg aattgttgaa taaaactgca ttgggtactc cacacattgc
     7681 cggatattcg gccgaaggca aagccaacgc tgcttctcaa agtgtgcggg cggtaagcaa
     7741 attttttggt ctgggacttg ataattggga agtctctaat ctccctgaac ctactaaagt
     7801 cgatttgtcg tttgttaata acatagaaga tttttttctg aaaacttaca atatcgaatc
     7861 agaaagcaat cagttaaaaa atcatcctcg actgtttgag tatctgagat ctcactaccc
     7921 ttttcgacgt gagccggatg cttacctgcc acaattgaat gaaattatgc agaaacagtt
     7981 aatcgaacaa tttcctcttt tttttaatca aaagaatttc aaaacgcttg cctaaggatt
     8041 atttattcta ataagataaa ttaatgggca actgctaata aaatcagaga tggtatgaaa
     8101 tacgtttgct cttacctgca aagaaaaagt ttgatggaaa ttacattcat aatatgttgt
     8161 taatctgtca ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaagtcttc
     8281 cataaatctg attttagctt cgtttataag atttgtaatt tccagatttt caaattcatt
     8341 atatttattt aaacaaaaag ctaatcctaa cagatgattt actattcctg ccaacatttg
     8401 ttgaaaacca cttttctgtt ctattgccac taaaattcct tgattataca actgaacagc
     8461 ttcgtccaat actcctacct tataaacggg attttcttta caaaaaaagc catttcctac
     8521 acgtttatct atattttctc ctttaaatcc aatccagtat tcttcccatc ctgttgctgg
     8581 atttgattta taattgtgcc attcgccagg gaaaagcatg aacatatttc ctgcatttat
     8641 attggtaagc ttgcaagaag ttgatgtgaa aattcctgaa cctttaacaa tatataataa
     8701 ttgatattca tccaatacac gaccttgttc ggtggaaaat aaatatccca taggatgtcc
     8761 atcttttggc ggataatctt cattgatgct taagctttgt ttaccaaccg tagttattgt
     8821 caatccccat cttgtatctt gctcgtttgc gatgagatag cggcaaagga ttgagttttt
     8881 tgcttctatg tttttcatgt attaatctgc ttgtaaaatt agagacaaag gaaacaagta
     8941 aatatctgaa aattaaacgt ctatttcttt tctccgatcc ttaaagattt gaaatttcat
     9001 gacaaaatta attttttttt tgaacttgtg aagggaaaaa tttttattcc acagattaca
     9061 agaaaaaaaa tttacatttt atgccaaatt tcaaagtatt tatatcaaaa tacttagtat
     9121 tttcttatgg ttattaatgt acttttgttg aaatttacat taggtagatt cattaataaa
     9181 ttttctgcat tcataataca agataaattc tatgagtaaa ctccctcatt tttttgctgt
     9241 agatctgggt gccaccagtg ggcgaaccat tttaggtact ttgcaaaata accgacttga
     9301 tttaaaggaa cttactcgtt ttcagaataa aattatttgt attcatgaca aatattattg
     9361 gaacatttat tcattatttg aagaaattaa aaaaggactt ttaattgcaa gtcagcaagg
     9421 aatccatatc aatgcaatgg gtatcgatac atggggagtt gattttgtgt atctggcaaa
     9481 cgatggttct ttacttaatc ttccgcgttc ctatcgtgat ccatatacta acggagtacc
     9541 ggaagaatat ttttcacgta tttcaaagag agaagtttat gaactgacag gcattcagat
     9601 tatgaattta aacagcttgt ttcaattgta tgcagcaaaa aaagaatctt cttcagctct
     9661 tgaatatgct gattcaattt tgtttatgcc cgatgctctt aattatttgt ttaccaagaa
     9721 aaaagtttgc gaatatacca ttgcttcaac ttctcaattg ttaaatccat tcacaaaaaa
     9781 aattgaagcc gaattattag ttccaatgaa tattgaacca acaatactac cacaaatggt
     9841 tatgccgggt acagttgttg gaaatttgga aaaaagtatt caacgggaat gcaatgttcc
     9901 tgaaattccc ttcatcgcag ttgcagagca tgatactgct tcggcagtgg ttgcagtgcc
     9961 ggcagaggac gaaaattttg cctacctgag ttcggggaca tggtcattaa tgggcattga
    10021 aaccaaaaat cccattatta cagaagaaag ttatcgttta aattttacca atgaaggtgg
    10081 agtagatggt accatacgat tcttgaaaaa cataacaggc atgtggcttt tggaaagatg
    10141 ccgcgaagaa tggaaattgg atgggaattc ctatacgtat cctgaaattg ttcaaatggc
    10201 agaatcagta aggagttttc aatttttaat tgatcctgat gacagtgttt tttcaaatcc
    10261 tgaaagcatg acacggtcaa tagctgtttt ttgtcggaat agtggtcaaa aagttcctgc
    10321 tacacatgct gaattcattc gttgtatctt tgacagtttg gcattgaaat ataagtatgt
    10381 tctcgattct ttgcaaaaat tgtctccttt cccaataaac cgacttcatg tcattggcgg
    10441 aggatctcaa aacaatttgc taaatcagct cacagcgaat gctataggac ttcctgtgat
    10501 tgcaggacct gcagaagcta ctgcgatggg aaacattatg atgcaggcaa aaggattggg
    10561 gctgatcaat tcattaactg aaattaggtc tatcatccga aattctgttg agctggaaac
    10621 tttttatcct caaaatacag agttatggga aaaagcatac aagaaatatc ttcatatttt
    10681 agaaaaataa aagtcgaaat aactattaca aaattaatgt atcatgaaaa aggatgaaat
    10741 tattcaaaag tcctacgaaa ttgcaaaaga acgttatgaa gcagtagaag taagtgttga
    10801 caaaacaata gaagttctcg aaaaattatc tctttccatt cactgctggc aagccgatga
    10861 tgtgacgggt tttgaaaatt ttggcaatat caacgatgca ggtattcaag ccactggcaa
    10921 ttatccggga aaagcacgca atatggatga acttcgtcaa gatatagaaa aagtgttggc
    10981 attggtagga ggtaaacatc gcttaaatct ccatgctatt tacggagatt ttcaacggaa
    11041 aaaaattgac cgcaacgaaa tagagccttc ccatttcacc agctggatgc aatgggctca
    11101 agagaaaggg ctaaaacttg attttaattc gacatctttt ggacatccaa aaagtggtaa
    11161 cctcacattg gcaaatcccg acaaagaaat tcgcaatttt tggattgaac acaccaaacg
    11221 ttgtcgacta attgccgaag aaatgggaaa atttcaacac gacccttgta ttatgaatct
    11281 ttggattcac gacgggatga aagatttaac tgtaaatcgg ttgctttaca gaagcatttt
    11341 ggaaaaatcg ttggatgaaa tttttgccaa aggttacaca aacatgaaag acagtataga
    11401 agcaaaactt tttggtattg ggttggaaag ttacacggta ggttcatatg atttttatct
    11461 ggcttatggt actaaaaacc aaaaaatagt tacgttggat acaggccatt ttcatttgac
    11521 ggaaagtgtt gctgataaaa tatcttctct tttgcttttt gttccggaaa ttatgttgca
    11581 tgtaagtcgg ccggtgcggt gggattccga ccatgtaacg atattaaatg atgatgttac
    11641 tgaattggct aaagaaattg tacgggcaga tgccttgagt cgagttcatg tggggttgga
    11701 ttatttcgat gcttctatca atcgcatcgg agcgtatgtt gttggcatac gggctacgca
    11761 gaaagctttt tttcaggcct tgctagaacc tgtttcttta ttgagaaaat atgaagataa
    11821 tggacaatat tttgaacggc tggcattgct cgaagaaacc aaaaatttgc cctggaatgc
    11881 tgtttgggac tatttttgct tgaaaaacaa tgtgcctgtc ggagaagatt atattgctga
    11941 aattcaacaa tatgagaaaa atgtaacctc aaaacgaaat taaatttcat cagaatataa
    12001 tttatttttt aaaggtctga tggaacccac atgtctgaat attatattca ttcgtgtttg
    12061 tgtatgcaat acatgtgggt ttcttgatta aaaatttttt aaaagccatt ttttatatca
    12121 tgaataccat cttaggatta ataattattg ccatcggtag tattggacaa tcaagttttt
    12181 atgtgcctat taaaaaagta aaaaattggt cgtgggaaag tttttggctg atgcaaggca
    12241 tttttgcatg gcttgttttc ccatttatag gctcattaat ggcaattcct gccggacatt
    12301 cgctgggaga aatatggtct gcgggtggta gtgatatgtg gaaatctgtt ggatatggta
    12361 ttctttgggg aattggaggc ttgacctttg gtcttagtat gcgttttttg ggaattgcgt
    12421 taggacagtc attagctttg ggaacatgct ccgcattcgg aacattgata ccggctttat
    12481 taaaaggtga taatctgttg aaaggcgatg gattgattct gttaatagga gtttgtcttg
    12541 ctattgccgg tattgcagtg attggttatg ccggttcgct aaaagcgaaa aatttaaccg
    12601 aagaagaaag aaaaaaggca gtaaaagatt ttgctttgaa aaaaggaatt ctcattgctt
    12661 tgctggctgg tattatgagt gcatgtttta atttggggtt ggaatccgga agctctataa
    12721 aaactgctct tctgaatatt ggagggaaca gtttaatggt actgaatccg gtgatttttc
    12781 tggttactat tggcggtttc ctaacaaatg cagtttattg tttttatcaa aattataaaa
    12841 atcaaacctt ctccgattac actcgatctt caggaaaaat ttggatgaat aatttgttgt
    12901 tttgtgcctt ggcagccgtt ctctggtatt cgcagttttt cggattgggt gccggtcaaa
    12961 gtttttttgc tcccggaagt gtcatgatgg cattttcatg gagtattctt atgtcgctga
    13021 atgtgttgtt tagtaatatt tggggaatta tattgaaaga atggaatggc gttggtaaaa
    13081 aaacaatttt tgttcttatt attgggttgg ccatattgat attctctatt atatttcccc
    13141 aaattgtaaa ataaacatgt aatttatcgc aaatggaatt gctcatcaat aggtacttga
    13201 cggttgcacc aagcaactta gtcagaaaca agcataagaa taggtatctt tctatctccg
    13261 aattttctac aaaagtttta ataaaattaa tttgacagat atgttattaa cttctcacac
    13321 atttcgacgg aaagtgaatt cttcaaaatt ttttcataat tttgcatttg ctggttgaat
    13381 ctactattga tgttttgtaa aaaaatattt tgaagttttg ataacaatcc ttattcttag
    13441 gctattttga aattttattt agaaaacatc aatctcattt tcatcagaat ataatttaat
    13501 tttttaatgg tctgatggaa cccaaatgtc taaatgttat attcatttgt ttttgtgtat
    13561 gaaatacatg tggttttcat aatggattta ttttttttgt aacatgcgtt tctaaataaa
    13621 aaattttttt cttttatcta attttacttt atttattaag tcaaattctt tcaaaaggaa
    13681 gaatatccca tttgaaagag acattttatt ttaattgttt atctatggca gttgtgttaa
    13741 ttatgcccaa acaagggcaa tcggtggaaa gttgtattgt aactgaaata aaaaagaaaa
    13801 aaggagacac cgtaaaaaaa ggagatattt tgtttt
//
LOCUS       DAOSIB010000060        13816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_4527_length_13816_cov_10.512245, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000060 DAOSIB010000000
VERSION     DAOSIB010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13816
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4527_length_13816_cov_10.512245"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..2642)
                     /locus_tag="PLD44_06340"
     CDS             complement(87..2642)
                     /locus_tag="PLD44_06340"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOF98987.1"
                     /translation="MNSFIIKQLKNYAILLLFILSFQALSAQKTEVPFSTDSNYLTVW
                     NGKKYIPIFIKGVNLGVAKPGSYPGELDVKPEQYRQWFEEIKDAGFNCIRLYTLHFPQ
                     FYNELLNYNTNHPQHPLFFFQGVWLNEEMEGYKNDLYQITDTFRAEIKENIDCVHGNK
                     LIAQRYGKAYGNYTADVSQWNIGYIVGREVYPEEILTTDSIHADNNHFTGTHFEINNA
                     SPSEVWFTENLNHLVEYEKNTYNTERPVSISSWPTLDPLHHAFEPNRTEDTATVDLSK
                     INILDAPAGLFISYHAYPYYPDFVGAQPDYKLASDNFGSNSYLGYIRDLKNHYRKYPL
                     LIAEYGVPSSWGIAHYTSSGMNHGGFDEQQQGETDLRLLRNISSSQLAGGIQFAWIDE
                     WFKKTWITDPIDFGNRILWHNITAAEQNFGLKKFVKSDEWKIWKNFDAADDITTLSAK
                     QNYDFFEIQLNLKDKMDLLGECWIAIDTYDAGLGESMMPNGQTIPSRAEFLLKITQYA
                     ATLYVTEAFDLFGLWHKILTPKQKLRSVATDGAPWNIVRWKNNSFYSDVQYIGELKLN
                     KSFQPETSKDAVTIYDDKISIRLPWTLLQFVDPSQKEVFHVDSLNPELRTRESDGIAV
                     SIFYKNKTYFSDTRFDWEKWNKVTDNDVEEKFKSSYWTMYENLTEFNTPAIAFPDNYE
                     LEGEESIFRIEENNGVLKNDFDMDGWNISAVLTDAPANGFVEINVDGSFSYIPRKGFK
                     GTDVFKYAIFDGTSLSQNTTVTLHVKKEGKQNPDNKKFFSLFPNPASDIVSIKSEINI
                     VSVRIFNYSGKLMEEFLPNDKTGTLNIFHYPAGLYIAMIDVDGQLFTEKFIKK"
     gene            complement(2654..3094)
                     /locus_tag="PLD44_06345"
     CDS             complement(2654..3094)
                     /locus_tag="PLD44_06345"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HOF98988.1"
                     /translation="MKSAESSDKKNSDPVIPFKFDIEPIDSQHQKLFRIFQRILDNIE
                     QKSGNTEQIIEELEEYTHYHFSTEEDLMKKFDYQEINEHIVQHEIFIQKMLDFKTSQL
                     FQSITLNEEIAGFLRKWFLNHILEYDAKYVDTFKKNLNSENEEK"
     gene            complement(3185..4636)
                     /locus_tag="PLD44_06350"
     CDS             complement(3185..4636)
                     /locus_tag="PLD44_06350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOF98989.1"
                     /translation="MEFPIIIEIVNWLIFVYACALFISYLLIALLSTSELKKYHSRNR
                     YSDFLKMLSFQKLPSVSIIAPAYNEEKTIVDNINCLLSLKYKDFEIIIVNDGSKDQTL
                     NKIIQYFDLIKTDMAYESQIITKNIRGIYKSRNNAYNHLTIIDKENGGKADALNAGLN
                     YAHNDLFLAVDVDSILEPDALLRMVKPFIEETKVKVIASGGVIRVANSCEIENGRIKK
                     VNFPKNQWAKYQTIEYFRAFTLGRMAWSRINGLLIISGAFGMFDRQTALAAGGYDTST
                     VGEDLELVVRMRRYMHEVEKKKYKIAFIPDPLCWTEVPESLKILSRQRNRWTRGAIET
                     IRKHMKMAFNPKYGRIGMISFPYWIFFEWLAPIIQFLGIGYVILLTIFGLLDVKVFLI
                     MTLFVLSFAVMYSFFSVLLEAIYYNKYKNVTYLSNLLFLSVMEMFVYQPINMIFSLSG
                     NIDYFFKKKSRHNWGNMTRKGFSGNTDQNSKQN"
     gene            complement(4642..5853)
                     /locus_tag="PLD44_06355"
     CDS             complement(4642..5853)
                     /locus_tag="PLD44_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98990.1"
                     /translation="MRTISLYLKFLFSRLIFIIALCLAVFLFFFLKQKEFILFNYSIG
                     NPLYSIIEQSPSWLYVVQVLIVIFLLLIVAELISIVLVTTRRSSAEKKNQLLHERIRE
                     VIFNYLIQDNKTRDINDFRIKAQPYLKSNHAQRVFINQLRKILILTKGEVHETCISLY
                     DALHFDPLIKSHFHSPYLRNKLFALKTAGDFQLTKYNRYLVRYMHSKNEMLRSEALQA
                     YVKANSDNDLSFLSDYQEKISLWDFNIIINAAKDRTNINFSALLKSHNPLIKSLGLRL
                     ANLQKKEELKPIIFKLMNDADPMVKEEAYLAFMALAKEKNDYLTLMYQFSCMTKNLQN
                     KVINLLINNEDKTSSYNFFEWIIENKPLEMKIRAMSKLLTMDMNLVLKYKNHPDENIR
                     KALRQLMDFYL"
     gene            complement(5857..7110)
                     /locus_tag="PLD44_06360"
     CDS             complement(5857..7110)
                     /locus_tag="PLD44_06360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HOF98991.1"
                     /translation="MKKKFQLIVFLIMLASTGFLQAQTNVDSIFAVAIEKAHGGAYAA
                     AIREAKTALSADTARGDICVFLANVYSWSQKNDSALLFIDKAKKLNYLNEEFYDSYLN
                     ILLRSGKYEELEAACEEAEKNNYGDTKNLLTKKLIAYAQTGAYDKGISLFKDEKNKVY
                     LQEKTIDELYTSLLMKQRTNLLSVNYSLDMFDNNDPQHLFSLGNAMQFKNRNIGLNAN
                     YAHRFGKNDVQIELNDYSVIYKKNYLYFNYGYALTGDLFPRHRTGLEYYFAISKHWDG
                     SLGGRYMFYPNAENKNVVIFTGNVGHYFGNNWVSLRPFYVVQNNLQSLSVVFKYRLYG
                     SDNRNFWGLEAGVGNSPDDILTTSQSSFNELMSYRIRLEKNFKVNRISDVNIGLGYMY
                     EQINQNATKQFRNRYLVEIGYKYRF"
     gene            complement(7135..7524)
                     /locus_tag="PLD44_06365"
     CDS             complement(7135..7524)
                     /locus_tag="PLD44_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOF98992.1"
                     /translation="MAYRINIISNEIVFLTLIRNFIKKIELDIQISETDSLNMLYKEL
                     KENSFDLIIVDGNFSENSAIDLLYIIRFKLKIFTPIWFFTVIQTPEYYNKALQVGANV
                     IIERPFDPIEIASQIHEFLHNKVSVKN"
     gene            complement(7566..8735)
                     /locus_tag="PLD44_06370"
     CDS             complement(7566..8735)
                     /locus_tag="PLD44_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98993.1"
                     /translation="MKKILIAILMLIVLMPLIMWLLWIIKKPRELNILILDKTVLTSA
                     VQEHLSLSWVINNEKFIHNSKSYNKQKDYYGFFPDDKGNYHIRDFENFSSENLDNLAN
                     SYDGAYYTDMYGIYTNEWIIRYYPEKIHDKRFIADRSKKIYGGMTQKELDFLRLMKQK
                     NKLIINEFNVIASPTSKEIRNKYENEFNIHWTGWVGRYFDNLDTLVNEDIPRWLIDNY
                     TRNHSGQWPFKKSGIAFVSENDQILILEKDTHLNTDIPYIYTNENNAKHYHLPEKIKY
                     PFWFDIMETNPSFRIISSYHLDTNAAGDSLLALGRIPKIFPAAVNYKNGYNYYYFCGD
                     FADNPISYASSRFRYIQVFSPLFYSSSKNERASFFWKYYRPLLRQILNEEYQRTE"
     gene            complement(8993..10909)
                     /locus_tag="PLD44_06375"
     CDS             complement(8993..10909)
                     /locus_tag="PLD44_06375"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOF98994.1"
                     /translation="MKKLLIFILSSIMIALTFSSCDYNEENFPGLDEMAAYKDVVNYE
                     YTISESDFTTIISALRANKNAQDSATATLLSSSKIFSVSAPASTLVPYVLKSKFYGAT
                     SGSAAKVTYQYKTGSYQYYLLTQADYKTIWQQDFIYALTPSKSPQSVLPGFLSQKFPA
                     AKEGDIKILEYSFSSADPVTSEVPLQYFKEDFESYEAGSGKTIPASAGNFFILNKDFL
                     GTRTWECRIFSNNKYAQVTSNASNTENQVWLITKQIDLTDANVPSLTFDVNVGYYNAD
                     CLSILISENFDGTEAGVASASWTDVTSSFTLPQTPTSGYGTLASAGTLELSAFKGKKI
                     NVAFKYTGDGRTDANPKKTTTYQVDNVVVSDTKTVSTVSDPKTQFSYYIYQNGQWNFV
                     NASFYQLAEEDYTSMGVATLNATTAPNYLPILLKIKFPFAQEGATKVVVYKTSATKNA
                     ADEYIYKSGVWSPISLIETRTEQFVFAGWENGGWIFDPTLRVTMKKGTNPTDDYMLVV
                     NYVRDTYGAQTPSLLGYYGTSLQSEYYYGFAAYYGNISWRESDRAKDPTYAALTTAEE
                     KQNYLVERTKEGLAVYLSLKFPDAQPKVSGVDLYCYVTTAIYNGVTTTNYVYKYQRVD
                     GEPFGWKYIESNPL"
     gene            complement(11013..13679)
                     /locus_tag="PLD44_06380"
     CDS             complement(11013..13679)
                     /locus_tag="PLD44_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOF98995.1"
                     /translation="MRKVFSFLFMMMLAQFAFSQASVKGVVVDKATNEPLVGVSVLQP
                     ALGIGTTTSLDGSFSLRLPEGKHELRFSYVGYSTKIVQISSNNVNLGVVQMESEAIGL
                     NDVTVTSSLAIRRKTPVALSVIEPLVIEEKLGTQEFPEILKSTPGVYATKQGGGFGDS
                     RINLRGFESANIAVMINGVPMNDMEWGGVYWSNWAGLSDVTRSMQVQRGLGASKVAAP
                     SVGGSINIVTKSTDAKKGGSFSYSIGNDGYNKISFNVSTGLSESGWAMTFLGAKTWGN
                     GYIQGTEFEAYSYFINISKIINEDQQISFTGFGAPQWHNQRYNGDKLLIPEWQKLKDG
                     YKFNPTYGFDENGQRKTGNYNHYHKPQLSLNHYWNMNDKSSLSSALYLSIGNGGGYGW
                     RGNTGTLMYGTNTATGLLNTTYRTLDGYMDYGKLQAENAANLNGSQAVIADSRNNHTW
                     LGLLSTFTTKFGQNIDFYGGLDLRYYAGMHDAVLVDLMGGKFFIDPARVNVKNSVNAG
                     NPAYVNEKLQVGDIVYRDNTGYVAQEGVFSQAEYSKGKLNVFLAGSLSNSTYWKVDRF
                     YYDNQKSDSKSFIGFTAKTGANYNLTDKHNVFANIGYISRAPFMSGGYFVTIHTSNAV
                     NKNAVNEKVFSTELGYGYRSKFLTANVNLYRTAWMDKTMVRSFGSNSDDGVVNLKGVN
                     ALHQGLEIDLISKPVKNLELTGMLSLGDWEWTSNATGYVFNKDGQPVNASGSTVDKNG
                     NTIQMFSADHAFVNLNLKGVKVGNSAQTTFALGAKYNFLKDFSAGLDYTHYARNYANF
                     SITANVGTTNYTTPWQIPAAGVFDFNANYKFKLQGFDASLNGVINNLFNQVYITDAQD
                     LTPTTSTSDEWKNTAVLYGFGRTYSISLRVRF"
BASE COUNT         4152 a         2587 c         2594 g         4483 t
ORIGIN      
        1 attgattttt tgaatttttt cagctcatta taaaacaaaa tatggaagaa agtttgttga
       61 aaagaacagg ctttcttcct tttttttcat tttttgatga atttttcggt aaagagttgt
      121 ccatccacat caatcatggc gatgtacagg ccggctggat aatggaaaat attcaaagtt
      181 cctgttttgt cgttcggcaa aaattcttcc attaattttc cggaataatt aaaaattcgt
      241 acagatacaa tattgatttc cgattttatg gaaacaatat cggaagcagg attggggaag
      301 agcgaaaaaa actttttgtt gtcgggattt tgtttgcctt cttttttgac atgtaaagtt
      361 acggttgtgt tttgggatag agaagtaccg tcaaaaattg cgtatttgaa cacatccgta
      421 cctttaaatc ctttacgagg aatataagaa aacgaaccat caacgtttat ttcgacgaaa
      481 ccgttggcag gtgcatcggt taaaacggca gaaatgttcc aaccatccat gtcaaaatca
      541 tttttcagta caccgttgtt ttcttcgatt ctaaaaatac tttcttctcc ctccagttca
      601 taattgtcgg gaaaagcaat ggccggagta ttgaattcgg tgagattttc gtacatagtc
      661 cagtaggaag atttgaattt ttcctccaca tcgttatcgg taactttgtt ccatttttcc
      721 cagtcgaagc gcgtatcgct gaaataggtt ttatttttgt aaaaaatgct caccgcaata
      781 ccatccgatt ctcttgtacg tagctcagga tttaaactgt caacgtggaa aacttctttt
      841 tgagacggat cgacaaattg caataaagtc cagggcaatc ggattgaaat tttgtcgtca
      901 taaattgtaa cggcatcttt tgaagtttcc ggctgaaagg atttattcag ttttaattct
      961 ccaatgtatt gaacatccga ataaaaagag ttgtttttcc agcggacaat gttccatgga
     1021 gcgccatctg ttgccacaga acgcaacttt tgttttggtg tgagtatttt gtgccacaaa
     1081 ccaaataaat cgaaagcttc cgtaacatac aaggttgctg catattgagt gattttgagc
     1141 aaaaattcgg cacgtgaggg gatggtttgt ccgtttggca tcatggattc tcccaaacct
     1201 gcatcgtacg tatcgattgc tatccaacat tctcccaaca aatccatttt atctttcaga
     1261 ttcaactgaa tttcaaaaaa atcgtaattt tgttttgctg acaaagtcgt tatgtcatca
     1321 gcagcatcaa aatttttcca gattttccat tcgtcggatt ttacaaattt tttcaggcca
     1381 aagttttgtt cggcagcggt tatgttgtgc cataaaattc tgttgccaaa atcaatagga
     1441 tcggtaatcc atgttttttt gaaccattcg tcaatccacg caaattgtat tcctccggcc
     1501 agttgactgg atgaaatgtt tctgagaaga cgcaaatcgg tttcgccttg ctgctgttca
     1561 tcaaatccgc cgtgattcat acccgaagaa gtataatgag caatacccca actggaagga
     1621 actccgtatt cggcaataag caaaggatat tttcgatagt ggttttttaa atctctgata
     1681 tagcccaaat aactgttgct gccaaaatta tccgatgcaa gtttataatc gggctgagcc
     1741 cctacaaaat ccggataata gggataggca tgataactaa taaaaagtcc ggccggagca
     1801 tccagtatat tgattttcga taagtctaca gttgccgtgt cttccgttct atttggttca
     1861 aaagcatgat gcaggggatc gagggtgggc caggaagaaa tgctgacagg tctttcggtg
     1921 ttgtatgtat ttttttcgta ttctaccaaa tgattcaaat tttctgtaaa ccaaacttcg
     1981 gatggtgaag cgttattaat ttcaaaatgg gtacctgtga agtgattatt gtccgcgtga
     2041 atactgtcgg tagtcagaat ttcttcagga tatacctctc gaccgacaat ataaccgatg
     2101 ttccattgcg aaacgtcggc agtatagttc ccataagctt ttccgtaacg ctgcgcaatc
     2161 agtttgtttc catgaacgca gtcaatgttt tctttaatct cggctctgaa ggtgtcggta
     2221 atttgataca aatcgttttt gtatccttcc atttcttcgt ttagccatac gccttgaaaa
     2281 aagaataaag gatgctgcgg atggttggta ttgtaattga gcaattcgtt gtaaaattga
     2341 gggaaatgaa gtgtgtaaag gcgaatacaa ttgaatccgg catcttttat ttcttcaaac
     2401 cattggcgat attgttcggg cttcacatcc agctcgcccg gataagagcc gggtttagca
     2461 acacccaaat tgactccctt gataaaaatg ggaatatatt ttttaccatt ccaaaccgtc
     2521 agatagttgc tgtctgtgga aaagggaact tctgtttttt gcgctgaaag agcttgaaaa
     2581 gataggatga acaaaagtag gatagcgtaa tttttcagtt gttttattat gaaactattc
     2641 atgtaatatt ttttcatttt tcttcatttt cggagtttaa atttttttta aaggtatcca
     2701 cgtatttggc atcatattcc aatatgtggt tgagaaacca cttgcgtaga aagccggcaa
     2761 tttcttcatt taaagtaata ctttgaaata attgagaagt tttaaaatcg agcatttttt
     2821 gtataaaaat ttcatgttga acaatgtgct catttatttc ctggtagtcg aattttttca
     2881 tcagatcttc ttcggtactg aaatgataat gggtatattc ttccagttct tcgataattt
     2941 gttccgtatt tccagatttt tgttcaatgt tatccaagat tctttgaaat atacgaaaca
     3001 gtttttggtg ttggctgtca attggttcaa tatcaaactt aaatggaatt acagggtcgg
     3061 aatttttttt atccgaactt tctgcgcttt tcataacgat tgttttttta atgtgctttt
     3121 attttctttt tcatataaat tcaagaaatc taaaaaattc agatttttac gtaaaatact
     3181 tatcttaatt ctgctttgaa ttttgatcgg tatttcccga aaaacctttt ctggtcatgt
     3241 ttccccagtt atgtctgctt tttttcttaa agaaataatc gatgtttccc gacaaggaaa
     3301 atatcatatt aataggctga taaacaaaca tttccattac agacaagaac aaaagattac
     3361 ttaaataggt tacattttta tatttgttgt aatatatagc ttctaaaagt acggaaaaaa
     3421 aggaatacat gacagcaaaa gaaaggacaa aaagtgtcat aatcaggaat acttttacat
     3481 ccaacagccc gaaaatagtc agcaaaatta cgtagccaat gcccaaaaac tgaataattg
     3541 gtgccaacca ttcgaaaaat atccaataag ggaagctgat cattccgatt cgtccatatt
     3601 ttggattgaa agccattttc atgtgttttc gtatggtttc gatggctccg cgtgtccagc
     3661 ggtttctttg tcgcgaaagt atttttagcg attcgggaac ttccgtccag cacaatggat
     3721 caggaataaa ggcaattttg tatttctttt tttctacttc gtgcatgtaa cggcgcattc
     3781 tgacaaccag ttccaaatcc tcgcctacag tggaagtatc atagcctcct gctgccaatg
     3841 ctgtttggcg gtcgaacatg ccgaaagcgc cagaaattat gagcaatccg ttgattctgc
     3901 tccaagccat acgccctaag gtaaatgccc ggaaatattc gatggtctga tattttgccc
     3961 attggttttt cgggaaattc acctttttta ttctgccgtt ttctatttcg caggagttgg
     4021 ctacacgaat tactccgccc gaagcaatta ccttgacttt ggtttcttca ataaaaggtt
     4081 ttaccattcg cagcaatgca tcgggttcga gaatggaatc aacatcaacg gccagaaaca
     4141 aatcattgtg cgcataattc aagccggcat tcagtgcatc ggcttttcct ccattttcct
     4201 tgtcgatgat ggtcaaatga ttgtaagcgt tgtttcgtga tttgtaaatt cccctgatat
     4261 tttttgttat aatttgtgat tcgtaagcca tgtcagtttt aatcaggtcg aaatattgaa
     4321 taattttatt gagtgtttgg tctttactgc cgtcgtttac aatgataatt tcaaaatcct
     4381 tgtatttcaa gcttaaaagg cagtttatat tatccacaat tgttttttct tcgttgtaag
     4441 ccggagctat aattgaaact gaaggcaatt tctgaaaact caacattttc aaaaaatctg
     4501 aatatctgtt tcttgaatga tattttttta gttctgatgt tgacaaaagc gctatcagaa
     4561 ggtaggagat aaaaagggca catgcataaa caaagatgag ccagttcaca atttcgataa
     4621 tgatgggaaa ttccataatt tttaaagata aaagtccatt aattgacgga gtgcttttct
     4681 gatattttcg tcagggtggt ttttgtattt caaaaccaaa ttcatgtcca ttgttagcag
     4741 ttttgacatt gccctgattt tcatttccag cggtttgttt tcgatgatcc attcaaaaaa
     4801 attgtacgaa cttgttttat cttcattatt gatcagaaga ttaataactt tattttgtaa
     4861 attttttgtc atgcaactga actgatacat taaagtcagg taatcgtttt tttctttggc
     4921 taatgccata aatgccagat aagcttcctc tttcaccatt gggtcggcat cattcatcag
     4981 tttaaaaata atcggtttaa gttcctcttt tttttgtagg ttggcaagtc ttaatcccaa
     5041 actctttatt aatgggttgt gagatttcaa aagagcggaa aaatttatat ttgttctgtc
     5101 ttttgctgca tttatgataa tgttaaaatc ccagagagaa attttttcct gataatccga
     5161 cagaaaactg aggtcgttat cggaattggc ttttacataa gcctgcagtg cttccgatcg
     5221 taacatctcg tttttactgt gcatatagcg gacaagatat cggttgtatt ttgtcagttg
     5281 aaaatcacct gccgttttca gagcaaataa tttgtttctg aggtatggag aatgaaaatg
     5341 actttttata agcggatcaa aatgcaacgc atcatacaaa gaaatacagg tttcgtgaac
     5401 ttcgcctttg gtgaggataa gaattttacg cagttgattg ataaaaactc tttgggcatg
     5461 attggatttc aagtagggtt gagcttttat acgaaaatca ttgatgtctc ttgttttgtt
     5521 atcctgaatc agatagttga aaatcacttc acgaatcctt tcatggagga gttggttctt
     5581 tttttcggca gaagatcttc gagttgtaac caaaacaatc gaaatcagtt cagcaacaat
     5641 cagcaacagg aagataacaa tcagcacctg aaccacataa agccatgaag gcgactgttc
     5701 gataatggaa tacaaaggat tgccaatgga atagttaaac aggataaatt ctttctgttt
     5761 cagaaagaaa aacaaaaata cggccagaca caaggctatg ataaaaatca accttgaaaa
     5821 aaggaatttc agatataagc tgattgttct catttttcag aatcggtatt tgtagccaat
     5881 ttccacaaga tagcggttgc ggaactgttt tgtggcattt tgattgattt gttcgtacat
     5941 ataacccaac ccgatattaa catctgaaat tctgttcacc ttaaagttct tttccaacct
     6001 gatgcgatag gacattaatt cgttaaatga actttgtgaa gtggtcagta tatcgtcggg
     6061 agaattgcct acgccggctt ccaagcccca aaaattcctg ttgtcgcttc cgtacagacg
     6121 atatttgaat acgaccgaaa gcgattgcaa attgttttgt accacataaa aaggtcttag
     6181 cgaaacccaa ttatttccga aataatgtcc cacattgccg gtaaagataa caacattttt
     6241 gttttctgcg tttggataaa acatgtatcg accgcccagt gagccatccc aatgcttgct
     6301 tatggcaaaa taatattcca gtcccgttcg gtgacgggga aacaaatcgc cagtaagagc
     6361 atatccataa ttgaaataaa gatagttttt tttgtaaata acggaataat cattcaattc
     6421 gatttgcaca tcattttttc cgaaacgatg tgcatagttg gcattaagtc cgatatttct
     6481 gtttttaaac tgcatggcat tgcccaaaga aaaaagatgt tgcggatcgt tgttgtcgaa
     6541 catgtcgagc gaataattta ccgaaagcaa attggtgcgc tgtttcatca gcagagaagt
     6601 gtaaagttca tcaatggttt tttcttgtaa ataaactttg tttttttcgt ctttaaacaa
     6661 gctgattcct ttatcgtatg caccggtttg agcataggca atgagttttt ttgtcaacag
     6721 gtttttagta tcgccataat tgttcttttc tgcttcttcg caggcagctt ctaattcttc
     6781 atatttccct gaacgcagca aaatattgag gtaactgtca taaaattctt cattgagata
     6841 gttcaatttt tttgccttgt ctataaacag taaagcagaa tcgttttttt gcgaccacga
     6901 gtaaacgtta gccaaaaaaa cacaaatatc accccgagca gtatcggcag acaaagccgt
     6961 tttagcttcc cgaatggctg ctgcataagc tccaccatga gctttttcta tggcaacggc
     7021 gaatatcgaa tcgacattgg tttgagcttg cagaaaacca gttgaggcaa gcatgatcag
     7081 aaaaacaatc agttgaaatt tttttttcat attgttggtt cgtttgtata actttcaatt
     7141 ttttacagag actttattgt gcagaaattc gtgaatttgt gaggcaattt ctatgggatc
     7201 gaatggcctt tcaataataa cattggcccc aacctgaagt gctttgttgt aatattcggg
     7261 cgtttgaata accgtaaaaa accagatagg agtaaaaatt ttcagcttga atcgaatgat
     7321 atacaacaag tcaatggcag agttttctga aaagttgcca tctacaataa ttaaatcgaa
     7381 gctattttct ttcagttctt tatacagcat gttgagagaa tcggtttcgc tgatttgaat
     7441 atccaattca atttttttga taaagtttct gattaatgtc aagaaaacga tttcgtttga
     7501 aattatattg attcggtatg ccatttaata attctttttt tgattctgaa ttttaaaaat
     7561 tgtgtttatt ctgttcgctg atattcttcg tttaaaattt gtcgaagcag gggacgataa
     7621 tatttccaaa aaaaacttgc tctttcattt ttggaagaag aataaaaaag tggtgaaaac
     7681 acttggatat aacggaaacg cgaacttgca taagaaatgg gattgtcggc aaaatcgcca
     7741 cagaaataat aataattgta gccgttttta taattcactg ccgccggaaa tattttgggt
     7801 attctgccaa gcgccaacag cgaatcgccg gcagcattgg tatcgagatg ataagaagaa
     7861 ataattcgaa aggaaggatt tgtctccata atatcgaacc agaaaggata tttgatcttc
     7921 tcgggaagat gataatgttt tgcattattt tcattggtgt aaatgtaagg aatgtcggta
     7981 ttcaaatggg tatctttttc cagaatcagt atctggtcat tttcactgac aaaagcgatt
     8041 cccgattttt taaaaggcca ttgtccgctg tggttgcggg tgtagttgtc gatcagccag
     8101 cgaggaatat cctcattcac gagggtgtcg agattgtcga aatagcgtcc cacccaaccg
     8161 gtccaatgaa tgttaaattc gttttcgtat ttgttgcgta tttctttcga ggtaggagag
     8221 gctatcacat tgaattcgtt aatgataagt ttattttttt gtttcatcaa tcgaagaaaa
     8281 tcaagttctt tttgtgtcat gccaccgtaa atttttttgg aacgatcggc aataaatctt
     8341 ttatcgtgaa ttttttcggg ataataccgg ataatccatt cattcgtgta aataccgtac
     8401 atatctgtat agtaagcacc gtcgtagcta tttgccaaat tgtccagatt ctcagaggaa
     8461 aaattttcaa aatctcgaat atgataattt cccttatcgt cagggaaaaa gccgtaatag
     8521 tctttttgtt tgttgtaaga tttgctgttg tgaataaatt tttcgttgtt gattacccac
     8581 gaaagcgaca aatgttcctg tacggcagaa gtcaatacgg ttttatccaa aatcagaata
     8641 ttcagctctc tcggtttttt aattatccac aaaagccaca ttatgagagg cattagtaca
     8701 atgagcatca gaatcgcaat cagtattttt ttcattcatt tatttaattt ttttcaatag
     8761 tttaacaaat agaaaaaata aaagttttac tttttagcca atttattgaa aatatttttt
     8821 aattttttcg aacattggta tcagcatggg atagatttat atggcaactg tttttggtac
     8881 aaagttattt ttttagaatg gttgaacaag gacaagctgc aaatatgctt aaccaaaaag
     8941 ccggcagata aacgccggct ctttgaaaaa acactaaaaa gtagaagaaa aattacaacg
     9001 gattgctttc tatatacttc cagccaaagg gttctccatc aacacgctga tacttgtaaa
     9061 cataatttgt tgtggtaacg ccgttgtaaa tagcagtggt tacataacaa tacaaatcaa
     9121 cgccgctgac ttttggctga gcatcgggaa atttcaaact cagataaacg gcaagtccct
     9181 ctttggtgcg ctcaacaaga taattctgtt tctcttctgc agtggttagt gctgcataag
     9241 tcggatcttt agctcggtcg gattcccgcc acgaaatatt gccgtaataa gccgcaaagc
     9301 cataataata ttccgattgc agactggttc catagtatcc cagcaaggat ggagtttgtg
     9361 ctccatatgt atctcttaca tagtttacaa ccaacatata gtcatccgtc ggattggtgc
     9421 cttttttcat ggttacgcgt aaagttgggt caaaaatcca accaccattt tcccaacctg
     9481 caaaaacaaa ttgttccgtt ctggtttcaa tcagcgaaat aggcgaccaa actcccgact
     9541 tgtaaatata ttcatcggct gcattttttg tggcgcttgt tttgtaaacc accactttcg
     9601 tagctccttc ctgggcaaac gggaatttga ttttgagcaa tatcggcaaa taatttggag
     9661 ctgtagtagc gttcaaagta gcaacaccca ttgaagtata atcttcttca gccaattggt
     9721 agaaagaagc atttacaaaa ttccactgtc cgttttgata aatataatat gaaaattgag
     9781 tttttgggtc tgaaacagtg cttaccgttt tggtatccga aacaacgaca ttatcgacct
     9841 gataagtggt agtttttttc ggattggcat ctgttcgccc atctccggta tatttgaatg
     9901 ccacattaat ctttttccct ttaaacgcag aaagctcaag cgtaccggca gaagctaacg
     9961 taccataacc ggaagttgga gtttgtggaa gagtaaaact tgatgtaaca tctgtccatg
    10021 aagctgaagc cacaccggct tccgtgccat caaaattttc cgaaatcaga atactcaaac
    10081 aatctgcatt atagtaacca acattaacgt caaaagttaa cgacggaaca ttggcatccg
    10141 tcaagtcaat ttgcttggtt atcagccaaa cctgattttc ggtgtttgaa gcattcgagg
    10201 ttacctgtgc atatttattg tttgaaaata tacgacattc ccacgttctt gttcccagaa
    10261 agtccttgtt cagaataaaa aaattgccgg cagaagcagg aatcgttttt ccagaaccag
    10321 cttcgtaaga ttcaaaatct tctttaaagt actgaagcgg cacttcagat gttacagggt
    10381 cggcagaaga aaaggaatat tccaaaattt taatatcgcc ttcctttgct gcagggaact
    10441 tttgcgataa aaatcccggc aacacacttt gtggagattt ggaaggtgta agtgcgtaaa
    10501 taaaatcctg ctgccaaatg gttttataat cggcctgggt aagcagataa tactggtacg
    10561 aacctgtttt atactgataa gtaacttttg cagcagaacc gcttgtagcg ccataaaatt
    10621 tgcttttcaa cacataaggc actaaagtgg aagcaggagc gcttacagag aaaattttgc
    10681 tactgctcaa taaagtggct gtggcagaat cttgggcatt tttattggct cgcaatgccg
    10741 aaatgatggt ggtaaagtcg gattcggaaa tcgtatattc gtaattgact acatctttat
    10801 aggctgccat ttcatccaac ccgggaaaat tttcctcatt ataatcgcac gacgaaaagg
    10861 tcaatgcaat cattatggat gacaggataa aaattaaaag ctttttcata cgtcgtaatt
    10921 ttaatttatt tgtaaaactt gctgaaaaat acctgaaaat ccagtttttt ttcttgagtt
    10981 gttttatctt aatgactaaa aattattttt acttaaaatc gaactcgcaa actgatagaa
    11041 taggttcttc caaaaccata caacaccgca gtatttttcc attcgtcaga agtagaagtt
    11101 gttggcgtca agtcttgagc atcagtaata tatacctgat tgaacaaatt attgatcacc
    11161 ccattcaagg aagcatcgaa tccctgaagt ttaaacttgt aattggcatt aaagtcaaaa
    11221 actcctgcag caggaatttg ccacggcgtg gtataattgg ttgttccaac attggccgta
    11281 atgctaaaat tggcatagtt gcgggcataa tgcgtataat ccaatcctgc tgaaaaatct
    11341 ttcaggaaat tatactttgc acccaaagca aaagtggttt gagccgagtt accaacttta
    11401 acacctttca aattcaaatt gacaaatgcg tgatcggccg aaaacatttg aatggtattt
    11461 ccatttttat ctactgtcga accacttgca ttgaccggct gtccatcttt gttaaacaca
    11521 tatcccgtag cgttgctggt ccattcccag tctccaagcg acagcattcc ggtaagttca
    11581 agattcttaa caggcttact tataaggtca atttccaaac cctgatgaag agcatttaca
    11641 cctttcagat tgacaacgcc atcatccgaa ttggagccga aagaacgaac catagtttta
    11701 tccatccaag ccgtacgata aagatttaca tttgccgtta aaaattttga acgataaccg
    11761 tatcccaatt cagttgaaaa aactttttcg ttgactgcat ttttatttac ggcattgctg
    11821 gtatgaatag ttacaaaata accgcccgac atgaacggag cgcgtgaaat ataaccgata
    11881 ttggcaaaaa cattgtgttt gtcggtaagg ttatagttgg caccggtttt ggcggtaaaa
    11941 ccgataaagc tttttgaatc agatttttgg ttgtcgtaat aaaaacggtc cactttccag
    12001 taagtgctgt tcgacaaaga accggccaag aacacattca gttttccttt ggaatattcg
    12061 gcctgcgaaa aaacgccttc ctgagccaca taacccgtat tgtcgcgata aacgatatct
    12121 cctacttgca gtttttcgtt gacgtatgca ggattaccgg cattcaccga gtttttaaca
    12181 ttcactcttg caggatcgat aaagaatttt ccacccatca aatccactaa tacagcatcg
    12241 tgcattccgg cgtagtaacg caagtcgagt cctccgtaaa aatctatatt ttgtccgaat
    12301 ttggtagtaa aagttgaaag caaacccagc caggtatggt tatttctcga atcggctatt
    12361 acggcttgtg aaccattcag attggctgca ttttctgcct gtaattttcc ataatccata
    12421 taaccatcca aagtacggta agtggtattc aacaaaccgg ttgccgtatt ggttccatac
    12481 atcaaagtgc ctgtgtttcc tctccaacca taaccgcctc cattacctat ggaaagatat
    12541 aaagccgaag aaagactcga tttgtcattc atgttccaat aatgattcaa tgacagttga
    12601 ggtttatgat aatggttgta attccccgtt tttctctgtc cgttttcatc aaaaccataa
    12661 gtaggattaa atttataacc gtctttgagt ttttgccatt caggtatcag cagcttgtcg
    12721 ccgttgtatc gctgattgtg ccattgaggc gctccaaaac cggtaaaaga aatctgttga
    12781 tcttcattta taatttttga aatatttata aaataggaat aggcttcaaa ctcggttcct
    12841 tgaatataac catttcccca tgtttttgct cccagaaaag tcattgccca gcccgattcg
    12901 gaaagtccgg tggagacatt gaacgaaatt ttattgtagc catcattccc aatggaatag
    12961 gaaaaagagc cacctttttt tgcatcggtc gatttggtaa ctatgttgat ggaacctccc
    13021 accgaaggag cagcaacttt ggaagcgccc aaacctcttt gaacctgcat agaacgggta
    13081 acatcgctta gtccggccca attcgaccag taaacgcctc cccactccat gtcattcatg
    13141 ggaacgccat tgatcatgac ggcgatattg gctgattcaa aaccacgcaa attgatacgt
    13201 gaatcgccaa aaccaccacc ttgtttggtt gcatatacac ccggagtgga ttttaatatt
    13261 tcaggaaatt cctgcgtacc cagcttttct tcaattacca aaggctctat tactgacaga
    13321 gcaacaggcg ttttacggcg aatagccaac gaagaagtaa cagtaacgtc attcaagcca
    13381 atggcttccg actccatttg taccactccc aaattgacat tgttagaaga aatttgaaca
    13441 atctttgtag aatagccaac ataagaaaac ctgagttcat gtttcccctc aggcaatctc
    13501 agagaaaaac ttccatcaag cgaagttgta gtcccgattc ccaaagcagg ttgaagaaca
    13561 cttactccaa ccaaaggttc gttggtagct ttatccacta ccaccccttt aactgaagct
    13621 tgcgaaaatg caaactgcgc aagcatcatc ataaaaagaa atgaaaatac ttttctcata
    13681 agaattctct tttttagttt ttaatttttt aagataagca tggcaaatat attctatttt
    13741 tttaatatca catatcatta aaaccgtaaa ttcaagtaat taatgcaact ttttcagggg
    13801 aaataaaagg taaaga
//
LOCUS       DAOSIB010000061        12994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_4862_length_12994_cov_12.588995, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000061 DAOSIB010000000
VERSION     DAOSIB010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12994
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4862_length_12994_cov_12.588995"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1478)
                     /locus_tag="PLD44_06385"
     CDS             complement(90..1478)
                     /locus_tag="PLD44_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF98996.1"
                     /translation="MKRNFLNYFVISSVMVSGLIFFTACDDKSDPDPEPTPTSNEVIL
                     NSDITGTRNLVNDSVYILQGQVNVSGTINIEKGTIIKGDKLTKGCLVVLRGGVINAIG
                     TPTEPIVFTSRMEPGLRNAGDWGGIIIVGNAPVNQSNPSVEGLTREVTYGGGTNPADN
                     SGSLRYVRIEFAGIALQPDKEINGLTLCAVGSGTTIDHIQVSYSGDDSFEWFGGTVNA
                     KYLIAYCGLDDEFDTDFGYSGRVQFALGVRNPRVADVSTSNGFESDNDGSGTGATPQT
                     SAVFSNVTLIGPYKTTADKNVNSMFGAGMHIRRNSSISVFNSVFAGWNTGLLLDGTST
                     YANATAGNLVIQNCVLSGTKGTAVNGAGGVTADQALTFFNSGNNQIIADNATTGIASS
                     FYDATKTGAEAAPTSFLPAAGSPLLSGASFTHAKLADNFFDKTPTFKGAFGTTDWTKE
                     QWTNFDPQNTVY"
     gene            complement(1712..4510)
                     /locus_tag="PLD44_06390"
     CDS             complement(1712..4510)
                     /locus_tag="PLD44_06390"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOF98997.1"
                     /translation="MKKTSVLRSVAIILATFFSSLLFAQSAIEGKVLDKKTKEPLVGA
                     SILIEGTSRGTTTDIDGNFKISELDPGKYKLIVSYVSYKKLEFDEIKIQKNIPTILNI
                     EMEEAELMLQSVQVVAQHRTDTELSLLKSVRTAVQVVNGISSQQIGKTLDRDAAEVVK
                     RIPGVTVQNDRFVVVRGLNQRYNNVWLNNASTPSSETDVKAFSFDAVPSNMIDNIMIY
                     KTGSPELSAEATGGFVKIFTKNIPEENFQQIEYSFGYNDQTSFKNSYHLPTQTLDVFG
                     LGSLQRDLPADFPANLNSLSIQQRDAAALKLNNHWVAQPYTALPNQKLTFSIGRKWNL
                     KKGNKIGNLTSLTYSNSSSSRFNMENNMYERFDPIAETPVYLYNYAADIYSTDFKIGL
                     MHNWAYIKTNGTKIEFKNLLNQIGVDKTSNTFGWNNYRQGNFKYFSNQYSARTTYSGQ
                     LSGNHKLKNNEDNKLDWNVGYAYANRIEPDRQNWSMKETQGGIYEYVLPDVPSINELG
                     RLYMNNHENVMVSGINHERKFQFLNTLPVLKSGLYGEFKSRNFSERSITYRKAYGPLG
                     NDEINALDFESLFTETYLGQGKVISVDEQTNVANTYKAQNWLGAGYLSMTLPAGKFNL
                     SGGIRAEYNHLLLDGYYNPTSPVHVDNPTFNIFPSINTSYNLSDKSLIRLAYAHTINR
                     PEFREVSPLSYYDFTEKNSVRGNPNLQDASIQNIDLRFENYPSPTETFSLAAFYKHFT
                     HPIEMVNNGVGNDFSFGNAQAAVNYGLELEAKKSLEKLIGLKNFTLQVNASYIFSKVE
                     FADKANEQNRPLQGQSPYVVNAALFYQNDKAGLSTSVMYNVMGKRILAAAQLNQGEVV
                     IPDIYEMPRHVVDFTLNKKLGNQMELKFGIKDLLAQNYKTQQTYEYEKDGTAKKATLT
                     NQSYNLGRTFSLALNWKF"
     gene            4978..6618
                     /locus_tag="PLD44_06395"
     CDS             4978..6618
                     /locus_tag="PLD44_06395"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOF98998.1"
                     /translation="MKNFTQFGKFLLFLFFFFSILLLTPAKATDFYFYVQFADKNNSP
                     YRLENPQEFLSERAIERRNFFSLSVDSTDLPVNPSYLSQVMNLGGEIHSQSRWLNGAT
                     VVMQDSALIEQVVSLPFVKWVQYTGKKSTVSNVPKQPSKFSPAIDYGLSGSQIAQVKG
                     NFLHELGYFGKNIVVGVLDAGFLNVDVNVAFDSLRLHNRLLGTKDFVGGSDLYRGNFH
                     GGNVLSIMAGNLPQNYLGTAPDASYWLIRTEDVDTEYLKELDFWVAGIEFADSVGSDV
                     VNSSLGYFTFDDSAMNFTYADMNGEVSRAAKAASMAAQKGMIVVCSAGNEGDKTWKYI
                     STPADAKGIITVGGVTADGTPSVFSSFGPSADGRVKPEICARGTQTSLINSSGNIVAG
                     NGTSYSSPLICGLMACYLQYFKEQHKLISIPQLLQSVFVTGSLYGSPTDQMGYGIPDF
                     QLAFLSTAFNGLNNQTQNQPQWLVDEKNKTIQVNFAGKTDNSAINVQIHAMDGKLLLS
                     RFYRSSSIVLSIENLSSGIYLVSVENGGKIFSKKILVP"
     gene            6673..7920
                     /gene="xseA"
                     /locus_tag="PLD44_06400"
     CDS             6673..7920
                     /gene="xseA"
                     /locus_tag="PLD44_06400"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOF98999.1"
                     /translation="MNENQNMTSLSLSELAAAIEETIRLNFDRPVWVRAEISELRENS
                     NGHCYMELIEKDENSDNLLAKVKATCWASVYRLLKPYFENATGQTFRAGLKVLLSVTV
                     EFHPVYGLSLTVRDIEPTFTIGEMAARRMQIIRRLEADGIADMNKQIPLPRLVQRLAV
                     VSSPTAAGYGDFCDQLKNNSGNFVFYTRLFPALMQGEQAESSIIAALEKIFRHAELFD
                     AVVIIRGGGATTDLSCFDSYELAVNCAQFPLPIITGIGHQRDVSILDLVAHSSLKTPT
                     AVAEFLIDRLQEAENLVKYTASDIYSFSKNYLDASLREINEKKLLIRQVLRNSLMRKQ
                     NEINRRISRLKTAVRMKLLREQNRLTLMEKSIETHSPAFMLKYGYSFTTLHGKKIFSV
                     HEVKPGDHVQTYLHDGSFESEVI"
     gene            7966..8331
                     /gene="folB"
                     /locus_tag="PLD44_06405"
     CDS             7966..8331
                     /gene="folB"
                     /locus_tag="PLD44_06405"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOF99000.1"
                     /translation="MAFIYLENMEFYSFHGCLEEERVKGNTFLVSVCMEVDTEKAGKT
                     DELSDTVNYQQVYDIVKKEMEIPSHLIEHVAQRIYDRIFEQIPQISGLHIRLSKLNPP
                     LGGKVEKASVEISGKRSDR"
     gene            complement(8342..10261)
                     /locus_tag="PLD44_06410"
     CDS             complement(8342..10261)
                     /locus_tag="PLD44_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOF99001.1"
                     /translation="MKKILLITGIICGINVFRFSGQVTTDTLSVQLSDVEINATRTKL
                     YPEMGRILTVIDKNEIERSAVRSIDELLDYVSGLDIRQRGKNGVQADISVRGGSFDQA
                     LILLNGINITDPQTGHFNLDIPIQLSDVSKVEILKGSSARMLGPNAFSGAINIITEEG
                     TKPFLKAGLEAGSFGYGEQSVTTAFIQKPYTLLSLSHKSSDGYTNNTDFENSNIFLHS
                     KLNSPKFGNIQFQAALQKKGFGANGFYSLAYPHQFESTQTLMAGLRWELIRRNISYHA
                     QSYWRQHHDRFELFRNFEGAPSSYTQHNYHLTDVVGGKFDASVANSWGKITFGIEGRN
                     EHIFSTVLGNEMNKTKPDPYENGINFNHSANRLLGTAFIDYAKIFDRWYVSAGGALSH
                     ATVFGTNPYGGFDIAFHPNNMTKIFFSSNTAMRLPTFTDLYYRSPTQISNPNLKPEHS
                     TTWELGAGIQQKHWQADATTFYRKGKNIIDWVKEPDSTKWESRNLTEVNAVGLDMNLN
                     YSFSNSFLQKAGLSYSFVQMDKNAENFDSKYALDYMKHKLIATLQHKTVKNCSLNWKL
                     SFLDRAGNYTDFYTQQPVNYQPYFLLDMKGLWEVEKYAVYLDLNNIFDKTYADYGGIV
                     QPGFNFTAGIKWKFK"
     gene            complement(10505..10882)
                     /locus_tag="PLD44_06415"
     CDS             complement(10505..10882)
                     /locus_tag="PLD44_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99002.1"
                     /translation="MKIIPNNFHKTVRFRRWSRANYAVFRSLTCVVTIGVLAAGICEK
                     ALLKNAPSNVSKNDTTSINSLVQCKEEKFRAEEILENIQLNTPVIFSTEDGKCCLSCT
                     YICCCRLKGIHFFQSIFFVSRPY"
     gene            complement(10965..12800)
                     /locus_tag="PLD44_06420"
     CDS             complement(10965..12800)
                     /locus_tag="PLD44_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOF99003.1"
                     /translation="MKKQNFIFAGVFCLFFFNFSLTANSTNQPPSWAKYAIWYQIFVE
                     RFYNGDTSNDPQPKYMTVSDMNIVPPHGWKISSWTGDWFERDSWMKNSNKTFSELLQY
                     RRYGGDLQGVLEKLDYLQELGINAIYLNPINDAPSLHKYDARNYHHVDINFGPDPEGD
                     VKIISGENPADPNSWRWTAADKLFLKLIEEVHKRGMKIIVDYSWNHTGTLFWAWRDIL
                     KNQAASPYKTWYNITRFDDPATPENEFAYEGWYGNAYMPEIRKTNLKSKRVNGQPYEG
                     NLDENAKRHIFEITKRWLSPNGDITKGVDGFRLDVAEQIGLEFWREYRAWVRSINPDA
                     YLIGEIWWEKWPDKMMNPAPYTQGDIFDAVMFYQVYCPARYFFAKTIGEIDAAQFRDS
                     LLFQWNRLKHENLYAMMNVSSTHDTPRLLTDFFNTNRYKFHATINDDPFYKTGKPDKL
                     TYRRLKLYLVHLFTTIGAPQIWNGEEMGMWGADDPYCRKPLWWKEFNFHPENRNNILP
                     LKAVYDPVKFDNNQFEFYKKLIAIRNVNPVLADGELEFVLTKNKTLAYKRFDRETEIL
                     VFFNLENTKQNFSLSGQYVDLLTGKKVKNKLLLPSLSATILKKTN"
BASE COUNT         3779 a         2509 c         2515 g         4191 t
ORIGIN      
        1 aaatctgata tgtgtttaaa acacttacat agaattaaaa agggcatccc gattttagaa
       61 tgcccttaaa aaaacagacg aaaaaatgat taataaactg tattctgtgg atcaaaattg
      121 gtccattgtt cttttgtcca atctgttgta ccaaaggccc ctttaaaagt tggagttttg
      181 tcgaagaaat tgtcagccag ttttgcatgg gtaaatgatg ctccactcaa caatggcgaa
      241 cctgctgcag ggaggaaaga ggtaggggca gcttcagcgc ctgttttggt tgcatcgtag
      301 aaagaactgg caattcctgt tgtagcatta tctgcaatga tttggttatt cccgctgttg
      361 aagaatgtca gagcctgatc agcagttact cctcctgctc cgttcacagc tgttcctttt
      421 gtaccggaca atacgcaatt ttgaattacc aaattgcctg cagttgcatt ggcataagta
      481 gatgtcccat ccagtaacag gcctgtattc catcctgcaa aaaccgaatt aaagactgaa
      541 attgagctgt tgcgacgaat atgcattcct gctccaaaca tgctgtttac atttttatcg
      601 gctgttgttt tgtaaggtcc tatcagagta acatttgaaa atactgcaga agtttgaggt
      661 gttgcgccag taccagagcc atcgttatcc gattcgaaac cgttggaagt tgaaacatcg
      721 gctacgcggg gattacgaac tcctaaagca aattgaacac gccccgaata accgaaatcg
      781 gtgtcaaatt catcatccaa tccgcaatag gctatcaaat attttgcatt taccgtacca
      841 ccaaaccatt cgaaagaatc atctcctgaa taggaaactt ggatatggtc aatggttgtt
      901 ccacttccta ccgcacacaa agtcagtccg ttaatttcct tatctggctg cagggctatt
      961 ccggcaaatt caatgcgaac ataacgaagt gaaccggaat tatcggctgg attcgtacca
     1021 ccaccgtaag taacttcccg agtcaaacct tctacacttg gattggattg gttaaccggt
     1081 gcatttccta ctataatgat tccaccccaa tcacctgcgt tgcgtaatcc gggttccatg
     1141 cgtgaagtga aaactatagg ttctgtggga gttccaatag cattgattac accaccacgc
     1201 aaaacaacca aacaaccttt ggtcaatttg tcacctttaa taatggttcc tttttcaata
     1261 ttgatggttc cacttacatt tacctgtcct tgaagaatat aaacggaatc gtttaccaaa
     1321 ttgcgtgttc cggtgatatc cgaatttaag attacttcat ttgaagtagg ggttggttcc
     1381 ggatcgggat ctgacttgtc atcacaagcc gtaaagaaaa ttaaaccgga aaccatgaca
     1441 gaagaaataa cgaaataatt taaaaaattc cttttcataa acgtgttgat taaattttac
     1501 atattgaaac ttaccgtaga tgctcggctt attctaaaat tttttttata ggacaaaagt
     1561 aatggttgac tgttacaaga ttattccgaa acaatgatgg ttatatgtaa aagatatgaa
     1621 gaatgtatta caggaatcat gatgtcatac gtttcgaaaa actcaaaatt ctcaaacagt
     1681 ttgccgattc catataattt gacagacaca attaaaattt ccaattgagt gccagcgaaa
     1741 aggttctgcc aagattataa ctttgattgg tcagcgttgc tttttttgct gtaccatctt
     1801 tttcatactc ataggtttgc tgagttttgt aattttgagc aagcaagtcc ttgataccaa
     1861 attttaactc catctgattg cccagctttt tgtttaatgt gaaatcgacc acatgacgag
     1921 gcatttcata aatatcgggg attactactt ctccctgatt caactgtgct gcagccaaaa
     1981 ttctttttcc catcacattg tacatcacag aagttgacaa accggcttta tcattctggt
     2041 aaaataacgc tgcgttaact acatacggcg attgtccctg caatggtcga ttttgttcgt
     2101 ttgccttatc ggcaaattcc accttactga aaatgtagga tgcattcacc tgaagagtaa
     2161 aattctttaa cccgataagt ttttccagcg atttttttgc ttccagttcc agtccgtaat
     2221 taacagccgc ttgggcgttt ccgaaagaga aatcgtttcc tacgccattg tttaccattt
     2281 cgatcgggtg cgtgaaatgc ttgtagaaag ctgccagcga aaaagtttct gtcggagatg
     2341 gatagttttc aaatcgcaaa tcgatatttt gaatggaagc atcctgcaaa ttgggattac
     2401 ctcttactga atttttttcg gtaaaatcat agtaactcaa aggcgaaact tcacgaaatt
     2461 cggggcgatt gatggtatga gcataagcta acctgataag agatttgtcg gataaattgt
     2521 aagaagtatt tatggaagga aaaatattga aagtgggatt atcaacgtgc actggtgaag
     2581 ttggattgta gtagccatcc agcagcaaat gattgtattc tgctctgatt cctccactca
     2641 aattgaattt tcctgccggc aatgtcatgg acaaatatcc agcaccaagc caattctgtg
     2701 ccttgtaggt gttagcaacg ttggtttgtt catctacgct aataactttt ccttgtccga
     2761 ggtaagtttc agtgaaaaga ctttcaaagt cgagtgcatt aatctcatca ttacccaatg
     2821 gtccgtatgc cttgcgatag gtaatgcttc gttccgaaaa attgcgcgat ttgaattccc
     2881 catagagtcc tgatttcaaa acaggcaacg tattgagaaa ttggaatttt ctttcgtgat
     2941 ttatcccaga caccataaca ttttcgtgat tgttcatgta aagccttccc aattcgttaa
     3001 tgctgggaac atcaggcaac acatattcgt aaattcctcc ttgagtttct ttcatgctcc
     3061 aattttgacg atccggttcg attcggttgg cgtaagcata acccacattc caatccagtt
     3121 tgttgtcttc attgtttttc aacttgtggt taccgctcaa ctgtcctgaa taggtggtac
     3181 gtgcagaata ctgattgctg aagtatttga aatttccttg acggtaattg ttccagccaa
     3241 aagtattgga agttttatct actccaattt ggttcaacag gtttttaaat tctatttttg
     3301 ttccatttgt tttaatgtaa gcccaattgt gcatcagtcc gattttaaaa tcggtactgt
     3361 agatgtcggc tgcatagttg taaagataaa caggtgtttc tgctatgggg tcaaagcgtt
     3421 cgtacatgtt attctccata ttgaaacgtg aagaagatga gttgctgtag gtgagtgaag
     3481 taaggttacc tattttgttc ccttttttca aattccattt tcttccaata gaaaaagtca
     3541 atttttgatt tggcaatgcc gtgtaaggtt gagcaaccca gtggttgttc agttttaaag
     3601 ctgcagcatc ccgctgttga atgctgaggg agttcaaatt tgcggggaag tcggcaggaa
     3661 gatctctttg caggctaccc aaaccaaaaa cgtccaatgt ttgtgttggt aagtgataac
     3721 tgtttttaaa tgaagtctga tcattgtatc caaaactgta ttcgatttgt tgaaaatttt
     3781 cttccgggat atttttggta aaaattttta caaatcctcc cgttgcttcg gcagaaagtt
     3841 caggagaacc tgttttataa atcataatat tgtcaatcat gttgctcgga acagcgtcga
     3901 aggagaatgc tttcacatct gtttcgctac ttggtgtgga agcattgttg agccaaacat
     3961 tattgtaccg ctgattgaga ccgcgtacta ctacaaatcg gtcgttttgt accgtaacgc
     4021 cagggattcg cttcactact tctgccgcat cccgatccag tgtttttccg atttgctggg
     4081 aagaaatgcc gttaaccacc tgaacggccg tgcgtacact tttcagcagc gataattctg
     4141 tatccgtgcg atgttgtgcc actacctgta cactttgaag cattaattcg gcttcttcca
     4201 tttctatgtt taaaatcgta ggtatatttt tttgaatttt aatttcgtca aattcaagtt
     4261 ttttataaga aacgtacgac actatcagtt tatattttcc gggatcaagt tcgcttattt
     4321 tgaaatttcc gtcaatgtcg gtagttgttc cccgtgaggt tccttcaatt agaatactgg
     4381 cacctaccag tggttctttg gtttttttgt ctaaaacttt tccttctatc gcactttgag
     4441 caaacagaag cgaggagaaa aatgttgcta aaattattgc aacagagcgt aatactgaag
     4501 tttttttcat ctgatttttc ttctgaattt ttatgctgca aaattaattc acaactgttt
     4561 caaaattgat aaaatgatat tacaatgcgg ttataatgat tttttgaaag aatattgaaa
     4621 tacctttttc tattttaaca tgagtttgag ataagtatat tttataagca attttaaata
     4681 agctataaag gttgttgaaa agtaagtatt cacattttat ctaaggttaa ttgtttataa
     4741 ataaggtttt tattttcaga aagtgaacct tattttttta aataaaaacc ttagtaacga
     4801 taatcaacaa caggaataaa ccttattacc ttattaatta tcaatttttt tgtatgatta
     4861 aaacatgtaa tcgttctatc gaacttacat tattaatttt ttttgcaaag aagcactttc
     4921 aaaattcgtt ttattgaatt attccggtat ttttgtacat aaaaaaatcc gtaacatatg
     4981 aaaaatttta cccaatttgg caaattcctt ctttttttgt tttttttctt ttcgatactt
     5041 ctattgactc cggcaaaggc aaccgatttc tatttctatg tgcagtttgc cgataaaaat
     5101 aattctcctt atcgattgga aaatccgcaa gaatttcttt ctgaacgcgc aattgagcga
     5161 cgcaatttct tttcgctttc cgttgattca accgatttgc cggttaatcc ttcctatctc
     5221 tcacaagtga tgaatctggg tggcgaaatt cattctcaaa gccgatggtt gaatggagca
     5281 actgtggtca tgcaggattc tgcgctgatt gagcaagtag tttcccttcc gtttgtaaaa
     5341 tgggttcaat acacaggaaa aaaatctacg gtttcgaatg ttccgaaaca gccttcaaag
     5401 ttttctccgg ccatcgacta tggactttcc ggttctcaaa tagctcaggt aaaaggaaat
     5461 tttcttcacg aattgggata ttttgggaaa aatattgtgg ttggagtgct ggatgccgga
     5521 tttcttaatg tagatgtcaa tgttgctttc gacagccttc ggttgcataa ccgattgctt
     5581 ggaacaaagg attttgttgg tggttcagat ctgtatcgtg gaaattttca cggaggaaat
     5641 gtgctttcga ttatggcagg aaatttacct caaaattatt tgggaacagc tcccgatgct
     5701 tcttattggc ttatcaggac cgaagatgtt gacaccgaat acctgaaaga actcgatttt
     5761 tgggttgccg gaatcgagtt tgccgacagc gtcggctcgg atgttgtcaa ttcgtctttg
     5821 ggttatttta cctttgatga ttcggccatg aattttactt atgccgatat gaacggtgag
     5881 gtttcgcgtg cagctaaagc tgcttcaatg gctgctcaaa aagggatgat agtagtttgc
     5941 agtgccggca acgaaggcga caaaacttgg aaatatattt ctacaccggc cgatgccaaa
     6001 ggcattatta cggttggagg cgttacggcc gatgggacac ccagcgtctt ttcttcattt
     6061 ggaccctccg ccgacggaag agtaaaaccc gaaatttgtg cacgcggtac ccaaaccagt
     6121 cttataaatt cgagtggcaa cattgtagcc ggtaacggaa cttcctattc ttctccactt
     6181 atttgcggat tgatggcttg ttaccttcaa tatttcaaag agcagcataa actgatttct
     6241 ataccgcaac ttctacagtc ggttttcgta accggaagct tgtatggttc gccaaccgat
     6301 cagatgggat atggcatacc cgattttcaa ctggcttttc ttagtacagc gttcaacgga
     6361 ttgaacaacc aaacccaaaa tcaaccacaa tggttggtgg atgaaaagaa caaaaccatt
     6421 caagttaatt ttgcgggaaa aaccgataat tcagctataa acgttcaaat tcatgcaatg
     6481 gatggaaagt tgttgctcag cagattttat cgttcttctt ccattgtcct ttccattgaa
     6541 aatctaagtt caggtattta tcttgtttcg gtagaaaatg gcgggaagat tttttcaaag
     6601 aaaattcttg ttccctaaat ttgtcaagaa aaatatttct gttgtatctt tgaagtgaaa
     6661 aattctcaat gaatgaacga aaaccaaaat atgacttctc tttccttatc tgaactagct
     6721 gctgctattg aagaaaccat tcgtctgaat tttgatcgcc ctgtatgggt acgtgccgaa
     6781 atcagtgaat tgcgtgaaaa ttccaatgga cactgttaca tggaattaat agaaaaggat
     6841 gagaattccg acaatttgct ggcaaaagtc aaagctacat gttgggcttc agtgtaccga
     6901 ttgttgaaac cttacttcga aaatgctacc ggtcagacat ttcgcgctgg attgaaggtt
     6961 ttgttgtccg ttacggtaga atttcatccc gtttatggtt tgagccttac ggtacgcgac
     7021 attgagccga catttaccat tggcgaaatg gcagcgcgca gaatgcaaat tattcgtcgt
     7081 ttggaagctg atggcattgc cgacatgaac aaacagattc ctttaccgcg attggttcag
     7141 cgtctggctg ttgtttcttc acccactgct gccggttatg gagacttttg cgatcagctg
     7201 aaaaacaatt ccggtaactt tgtgttttat acccgccttt tccctgcctt aatgcagggc
     7261 gagcaagccg aaagctccat tatcgcagct ttggaaaaga tttttcgcca tgcagaactt
     7321 tttgatgcag tggtgattat tcgaggtggc ggtgcaacaa ccgatttgtc ctgtttcgat
     7381 tcctacgaat tggcagtgaa ttgtgctcag tttcccttgc ccatcatcac cggcatcggc
     7441 catcagcgtg atgtttctat tcttgatttg gtggcacatt cgagtttgaa aacacccacc
     7501 gccgttgccg aatttctcat agatcgcctg caggaagccg aaaatctggt taaatataca
     7561 gcttccgata tttattcttt ttctaaaaat tatctggatg cttcactcag agaaatcaac
     7621 gaaaaaaagc tgttgattcg ccaagttctg agaaacagtt taatgcgcaa acaaaatgaa
     7681 atcaatcgtc ggataagtcg tttgaaaacg gcagtacgga tgaaattgct cagagaacaa
     7741 aatcgattga ctttaatgga aaaatctatt gaaactcatt ctccggcctt tatgttgaaa
     7801 tatggttaca gtttcactac ccttcacgga aagaaaattt tttccgttca tgaagtgaaa
     7861 cccggcgacc atgttcaaac ttatctgcat gatgggagtt ttgagagtga ggtgatttga
     7921 cgcgaaaaaa taaagggaat ttattaaata ctcaattctt aacttatggc attcatttat
     7981 ctcgaaaata tggaatttta ttcctttcat ggttgcttgg aagaagaacg tgtaaaagga
     8041 aatacttttc tggtttcggt ttgtatggaa gttgatacgg aaaaggctgg aaaaactgac
     8101 gaactttcgg atacggtgaa ttatcagcag gtgtacgata ttgtaaaaaa agagatggaa
     8161 attccttccc atctcataga acatgtcgct caaaggattt acgaccggat ttttgaacaa
     8221 attccgcaaa tttccggatt gcatatccga ctttccaaat taaatcctcc tttgggtgga
     8281 aaagtagaaa aagcttcggt agaaatttcc gggaaacgtt ctgaccgtta atgatggttt
     8341 tttatttaaa tttccatttt atcccggcag tgaagttaaa tcccggctgt acaattccgc
     8401 cataatccgc gtaagttttg tcgaaaatat tattcagatc aaggtaaact gcatatttct
     8461 ctacttccca taatcctttc atatccagta aaaaataagg ttgataattt acgggttgtt
     8521 gcgtataaaa gtcggtgtag tttcctgctc gatccaaaaa ggagagtttc cagttgagag
     8581 aacagttttt cactgtttta tgttgtaaag ttgctatcag cttgtgtttc atgtaatcca
     8641 acgcatattt ggaatcgaaa ttttcggcat ttttatccat ctgaacaaaa gaataggaaa
     8701 gcccggcttt ttgaaggaaa gaatttgaaa aagaatagtt caaattcata tccagcccca
     8761 cagcgttaac ttcagtcaaa tttcggcttt cccattttgt cgagtcgggt tcctttaccc
     8821 agtcgataat gtttttccct tttcggtaga aggtcgttgc atcagcttgc cagtgttttt
     8881 gttgaatgcc ggctcccaat tcccatgttg tggaatgttc aggttttaaa ttgggattgc
     8941 tgatttgcgt tgggcttcgg taatataaat cggtaaaagt tggtaacctc atggccgtat
     9001 ttgatgaaaa aaatatcttg gtcatgttgt ttggatgaaa agcaatgtca aatccgccat
     9061 acggattcgt tccgaagaca gtggcatgtg acaatgcccc cccggccgaa acataccatc
     9121 gatcaaaaat ttttgcataa tcaataaatg ccgtccccag taaacgattt gcactatgat
     9181 taaaatttat tccgttttcg taagggtctg gtttcgtttt attcatctca tttcccaaaa
     9241 ctgtgctgaa aatatgttca tttcttcctt caataccaaa agtaattttt ccccatgaat
     9301 ttgccaccga agcatcaaat tttcctccca caacatctgt caggtgataa ttgtgttgtg
     9361 tgtacgacga aggagcccct tcaaagttgc gaaacagttc aaatcggtcg tgatgttgtc
     9421 gccaatagct ctgagcatgg tagctgatgt ttcttcggat aagttcccat ctcaaccccg
     9481 ccatcagggt ttgcgtactt tcaaactgat gcggataagc caacgaataa aatccgtttg
     9541 caccaaaacc ttttttttgt aaagctgctt gaaactgtat attaccaaat ttcgggctat
     9601 tcagtttcga gtgcagaaaa atgttgctat tctcaaaatc tgtattgttg gtataaccat
     9661 cgctgctttt gtgagataaa gaaaggagcg tataaggttt ttggataaat gcggtagtaa
     9721 ccgactgttc tccataacca aaacttccgg cttccaaacc tgcttttaaa aaaggttttg
     9781 ttccctcttc ggttattatg ttaatggcac cgctgaaagc attcggccct aacattcggg
     9841 ctgaagaacc ttttaaaatt tcaactttac tcacatcgga gagttgaatg ggaatatcaa
     9901 ggttaaaatg tcccgtttga gggtcagtaa tgttgatacc attcagcaaa attagcgcct
     9961 ggtcaaatga acctccacga accgaaatat cggcttgaac gccatttttg ccacgttgac
    10021 gaatatctag accggaaaca taatcgagca attcatcaat gctgcgtact gccgagcgtt
    10081 cgatttcatt cttgtcgata acagttagga tacgacccat ttcgggataa agttttgttc
    10141 gggtggcatt gatttcaacg tctgaaagtt gtaccgacaa ggtgtcggtg gtaacttgtc
    10201 ccgaaaaacg aaaaacattg attccgcaaa taattcctgt gattaaaaga attttcttca
    10261 tttgaaaaaa ttttagttaa acaatattta tatgaattct atataagaat tttttataat
    10321 taattataaa taagccatta aggttgaggg aaaaagtatt tacctgttgt tatggttagt
    10381 tgttcttaaa gtaataagaa tcaaccttat cagttctcaa ctttttatat gattaataca
    10441 ggtaatagct gtatcgtact tacgtaataa ttatgctttg attcatcttt ttttcacgat
    10501 ttgtttaata tggcctacta acaaaaaaaa tcgactgaaa gaaatgaatt cctttcagtc
    10561 gacaacaaca tatataagta catgaaagac agcattttcc gtcttcagta gagaatataa
    10621 caggagtatt gagttgaata ttttcaagaa tttcctccgc tcggaatttt tcttccttgc
    10681 actgaactaa agaattaatg gatgtagtat cattttttga gacattggaa ggagcatttt
    10741 tcagtaaagc tttttcgcaa attccggcag caagtacacc tatggttacc acgcaggtca
    10801 aacttctgaa aacagcatag tttgccctgc tccagcgacg gaagcgaact gtcttatgga
    10861 aattattggg aataattttc attgtttttt cagatttgac agggcaaatg taagcaaaat
    10921 tttttttaag ttatttgttt tttcaaaaga aacttaaatt tgtttcagtt tgtttttttc
    10981 agaatagttg ccgatagcga tggcaaaagg agcttattct tcactttctt tcctgtaagt
    11041 aaatcgacat attgtccgct caatgaaaaa ttttgtttgg tgttttccag attaaaaaac
    11101 accaaaatct cggtttccct gtcaaaacgt ttgtaagcaa gtgttttatt ttttgtcaaa
    11161 acaaattcca attctccgtc tgccaacacg ggattaacat ttcgtatggc aataagtttt
    11221 ttgtagaatt caaattggtt gttgtcaaat ttcaccggat cataaacggc tttcagggga
    11281 aggatattgt ttctgttttc cggatgaaaa ttgaactctt tccaccacaa cggcttccgg
    11341 caatatggat catcagctcc ccacatgccc atttcttctc cattccaaat ttgaggtgcc
    11401 ccgatcgttg taaacaaatg aaccagatat aatttcaacc gcctgtatgt cagtttatcg
    11461 ggtttccctg ttttgtaaaa cggatcatca tttatcgttg catgaaattt atatcgattg
    11521 gtgttgaaaa aatcggtcag taaacgtggt gtatcatggg tggaagacac attcatcatg
    11581 gcatacaggt tttcgtgttt caatcgattc cactgaaaaa gtaagctgtc tctgaattgt
    11641 gctgcatcaa tttcaccaat tgttttggca aagaaataac gtgcaggaca atagacctga
    11701 taaaacatga cagcatcaaa tatatcacct tgcgtgtacg gggcaggatt catcattttg
    11761 tcgggccatt tctcccacca tatttcgcct atcagataag catcaggatt gatggaacga
    11821 acccacgcac gatattcccg ccaaaattcc aatccaatct gttcagctac atccaaccga
    11881 aagccatcaa cgccttttgt aatatctcca tttggagaca gccaacgttt ggtaatctca
    11941 aaaatatgtc ttttggcatt ttcatccaga tttccttcgt agggttgccc attgacacgt
    12001 ttcgatttca gattggtttt tctgatttca ggcatatagg catttccgta ccagccttca
    12061 taggcaaatt cgttctcggg tgttgccgga tcgtcaaaac gggtaatatt gtaccaagtt
    12121 ttgtaaggag aagcagcctg atttttcaaa atatcgcgcc aagcccaaaa taaagttccg
    12181 gtatgattcc atgagtaatc gacaattatt ttcattccgc gtttatggac ttcttctatc
    12241 aacttcagaa aaagcttatc ggcagcagtc catcgccaag aattcggatc ggcgggattt
    12301 tcgccggaaa taatttttac atcaccttcc ggatcagggc caaaatttat atctacatga
    12361 tgataattgc gtgcatcata tttatgaagc gatggcgcat cattaatcgg attgagataa
    12421 atagcattga ttcccaattc ctgtaagtaa tcgagttttt ctaaaactcc ctgcaaatcg
    12481 cctccataac gacggtattg aagcagttca ctaaaagttt tattcgaatt tttcatccaa
    12541 ctatctcgtt caaaccagtc gcctgtccat gatgaaattt tccagccgtg aggtggaaca
    12601 atattcatgt cgctaaccgt catatatttt ggttgaggat cgttggatgt gtcgccattg
    12661 taaaaacgct caacaaaaat ctgataccaa atggcatact ttgcccaact tggcggctga
    12721 tttgtggaat tagcagttaa agaaaaatta aaaaaaaaca aacaaaaaac acctgcaaaa
    12781 ataaaattct gttttttcat ggaaataata attttttaaa agcaagaata tcaatccaaa
    12841 aagttcatcg gcacatcgta taattttttc tctactatcc gaaactttag aaatttaaaa
    12901 tgcctgagaa tttcatattt ttatattcga ccaagaaaaa cagaaataat gaaactccac
    12961 aggcgcagta aaaataacaa aaaaccggtt atcc
//
LOCUS       DAOSIB010000062        12811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_4949_length_12811_cov_12.018031, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000062 DAOSIB010000000
VERSION     DAOSIB010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12811
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4949_length_12811_cov_12.018031"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..615)
                     /gene="efp"
                     /locus_tag="PLD44_06425"
     CDS             complement(46..615)
                     /gene="efp"
                     /locus_tag="PLD44_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOF99004.1"
                     /translation="MATTADIKNGMCIELDGQYYIITEFLHVKPGKGAAFVRTKLKNV
                     VTGRTIDKTFNAGVKIDEVRIERRPYQYLYQDDMGFNFMNNETFEQVSVNKELINGVD
                     FLKEGMICDVVTHAESETILYVDLPTHVTLQVTYTEPGLKGDTATNATKPATVETGAV
                     IRVPLFINEGELIKVDTRDGSYVERVKTV"
     gene            complement(814..>3561)
                     /gene="nifJ"
                     /locus_tag="PLD44_06430"
     CDS             complement(814..>3561)
                     /gene="nifJ"
                     /locus_tag="PLD44_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOF99005.1"
                     /translation="LFDYYGAPDADRVVIAMGSATEAIKEGVDYLNAKGEKVGLISVH
                     LYRPFSAKHFLSVLPETAKRICVLDRTKEPGASGEPLYLDVKDVLYAKSNQPTVVGGR
                     YGLSSKDFTPAQVLAVFENLALPEPKNHFTVGIVDDVTFTSLPLREEIPMGGKGIFEA
                     KFYGIGSDGTVGANKNSIKIIGDNTDKYCQAYFSYDSKKSGGFTCSHLRFGDEPIRST
                     YLVTTPDFVACHVQAYLRLYDVTKGLKKNGTFLLNTIWDEEEVKKNLPVKVKKYLAAH
                     NINFYIINATKIAQEIGLGNRTNTILQSAFFKISNVIPYELAVEKMKYAINKTYGKKG
                     EDIVNMNYAAVDRGGEYVKVEVPSNWANLEDEKEMEENFPPFIREVVMPINAQAGDDL
                     PVSVFKGREDGTWDSGTAKYEKRGVASFVPVWNSANCIQCNECAFVCPHAAIRPFVLD
                     EAEQAKAPFTDTIKANGKQFVGMTYRIQVDVLDCMGCKNCADICPGTKGNKALTMVPI
                     ETQYDNQKNWDFCISEVKSKQHLVDVKMNVKNSQLATPLFEFSGACAGCGETPYVKLI
                     TQLFGDRELVANATGCSSIYSASAPSTPYTTNENGQGPAWANSLFEDNAEFGYGMELA
                     TEVMRDRLERIMKEAIESNCCSDELKALFAEWIENKEDAEKTKELAAKITPLLKNCDC
                     EHCRKISELSHYLVKKSQWIIGGDGWAYDIGYGGLDHVIASGKNVNILVLDTEVYSNT
                     GGQSSKSTPIGAIAKFASAGKKIRKKDLGMMAITYGYVYVAQIAMGASKAQTLKAIRE
                     AEAYNGPSLIIAYSPCISHGIHNGMEKAQEEQERAVECGYWQLYRYNPDLEAEGKNPF
                     VLDSKEPQWEKFQDFLKGEIRYTSLMKQFPAEAQELFVAAENNAKWRYKMYKQMADRQ
                     F"
     assembly_gap    3562..3661
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3662..4209)
                     /locus_tag="PLD44_06435"
     CDS             complement(<3662..4209)
                     /locus_tag="PLD44_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOF99006.1"
                     /translation="MAKQKKFITCDGNQAAAHIAYMFSEVAAIYPITPSSTMAEYVDE
                     WAANGRKNIFNEKLQVQEMQSEAGAAGAMHGALQAGALATTFTASQGLLLMIPNMYKI
                     SGELLPGVFHVSARSIAAQALSIFGDHQDVMAVRQTGCAMFATGSVQEIMDMAAVAHL
                     SAIKSRVPFVHFFDGFRTSHEI"
     gene            complement(4255..5436)
                     /locus_tag="PLD44_06440"
     CDS             complement(4255..5436)
                     /locus_tag="PLD44_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOF99007.1"
                     /translation="MESFFRTHQYLVEHVQSPVRRTLMDEIDWTDRLIGIKGSRGVGK
                     TTFLLQYAKDFFRSNDQSCLYINFNNFYFTTHTLVEFAERFVKQGGRTLLIDQTFKYE
                     NWSKELRECYERFPELKIIFSGSSVMRLKEDNEDLQDVVKVYNLRGFSFREFLNLQAG
                     TNFQPIDLEDIIKNHVPIANEICSKIDPLLYFQDYLHHGYYPFFLEQRNFSENLLKTM
                     NMMIEVDILLIKQIELKYLSKIRKLLYLLMISAPATPNISQLSCEIQTSRATIMNYIK
                     YLKDARLLNLLYNDSEQFPKKPHLIYVHNTNLMHAAMPGNIDRLSEIKTFLYNSLHAK
                     YKVNSGKYHSDFTVDKQYTFKCELNSNISKQHSKLIFATDQLKIGKKNEIPIWLFGFL
                     Y"
     gene            5749..7152
                     /locus_tag="PLD44_06445"
     CDS             5749..7152
                     /locus_tag="PLD44_06445"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF99008.1"
                     /translation="MKIKFIGAAREVTGSKHLITTKDGKRILLDCGMFQGKGLETDAK
                     NRDLGFDPETIDHIILSHAHIDHSGLIPYLYRNGFRGSVICTNATRDLCSIMLADSAH
                     IQELDIKWFNKKRARQGLPPVEPIYDKEDAENCMDLFIGVSLNRRFYIDNKINVKFTN
                     TGHLLGSAVVNLEIIENGKKVHIAYTGDIGRSTDYLLRSPDPFPQCDYLIAESTYGNR
                     LHPETNQTEEDFLKIITETCIKNKGKLIIPAFSIGRTQELVFLLNNFYNRHLLPHIEV
                     FVDSPLSVNATEIYRMYSELMNKNVKEVLKYDNDPFGFNSLHYIKNVEESKALNTYDK
                     PCIIISASGMMEAGRIKHHVANNIENPRNTILIVGYCSPTSLGARIQQPGLREISIFG
                     EIHPVNAKICKIEAFSGHGDYKEMIDYLGCQSAGLIKKTFLVHGDYEVQVEYREKLQK
                     EGFRKVEIPDAKEEFIL"
     gene            7502..7756
                     /locus_tag="PLD44_06450"
     CDS             7502..7756
                     /locus_tag="PLD44_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOF99009.1"
                     /translation="MTKMITFNELRRIKDALPHGSLSQIAEELGLDIETVRNYFGGHN
                     YKDGKSCGIHIEPGPDGGLVMLDNTDILEAALRILGKEEA"
     gene            7996..9342
                     /locus_tag="PLD44_06455"
     CDS             7996..9342
                     /locus_tag="PLD44_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HOF99010.1"
                     /translation="MNLSPAFEKRMQVLLEEEYALFRAALEKPAPVSIRANNKIVYQP
                     SKEQVSWCESGFYLSERPVFTIDPFFHAGVYYVQEASSMFLRQCLKQYFSQAETVLDL
                     CAAPGGKTTLLSQYLNENALLISNEINRSRAYILAENVAKWGNANVVVTNNQPADFKN
                     LPGFFDAMLIDAPCSGEGMFRKNPASIDEWSLTNVENCVVRQRKIISEVWDTLKTDGI
                     LVYSTCTYNRQENEANIKWICEELGAEILRMDIQHFQGILETEAGYRFFPHKIKGEGF
                     FISVLRKTSPTPQKMRKAKKIEKKSDDKIKSDVGIKLLEPEKWNLLMENNRLRAVSKA
                     RQKEIAIIENNLKCLLSGIYLGDYKNSDFIPSAQSALSKETDLKAVETVEIDYTTAVR
                     YLKKENIQVESDKKGFLLLLYKGVPLGWVKNIGQRCNNLYPQEWRIRMKIEELEEI"
     gene            9339..10085
                     /locus_tag="PLD44_06460"
     CDS             9339..10085
                     /locus_tag="PLD44_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF99011.1"
                     /translation="MKILLLGTGTSTGVPQIGCNCYTCTSTNIKDQRLRSSLLVSEKN
                     TRILIDCGPDLRQQLLNYRIHDLTGILLTHEHYDHIGGLDDLRPLGPSHLFGEKRVLK
                     VIERNMPYCFGDKRYPGVPELFLHEINEEEFSLDGLQVQPVRVMHANLPIFGFRIGKM
                     AYLTDVKTLDEKAVEQLQQLDVLIINALRPFKHISHLSLSEAIELAEKINAKETFFTH
                     ISHDMGRHNEINSVLPPHIHLAYDGLELAI"
     gene            10182..11207
                     /locus_tag="PLD44_06465"
     CDS             10182..11207
                     /locus_tag="PLD44_06465"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HOF99012.1"
                     /translation="MKKSFIIFVGLSIGAMLFSSCSNMLYTSLDVLRPAKVAFLPNVN
                     RLLIVNNTPVMALENDPNKTKKNVTLPADSLPIYCLSVLAQELGNNSFFSSVLLEPNS
                     IYPKNNYSEKLSTEMVKKLCENYNCDVILTLDSLKASDVLTEYYLEDISQYLSTYEIY
                     LKSKWSIYYPSQSKPVSLQFKDTVYWEAQDYSRQRALKQMPDRWDALVDGALYVGKNS
                     VKRFIPYWDKVDRYFFNPGNKLMRQAMDSVYVRNWTSAIELWKKAMNKSKSEKLKGEA
                     ANNIAIAYEILGDMDNAIAYARQSLQYFSSVSIIDYDTMERINNYYYELIKRKNEIQQ
                     LKLQLGE"
     gene            11281..12807
                     /locus_tag="PLD44_06470"
     CDS             11281..12807
                     /locus_tag="PLD44_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOF99013.1"
                     /translation="MELLLPSDEILLQEKGIGKQQIEEQLHYFETGFPFLKIVAPAVI
                     GKGITIIDDKKGEFYINKWESYRQKEGIDVVKFVPASGAASRMFKDLYEFLESEAELP
                     QKSSVKDFFNQIEKFAFYEELNKACLKNEGKSVAELIKNNRQKTVVENLLLEKGLNYG
                     FLPKGLLLFHSYPDEKRTPVQEHLVEGALYATHADNDVKIHFTVSHQHRALFEKHIQE
                     ILEKYEQKFKVHYHIDFSEQKPSTDTLAVDMNNRPFRENGQLVFRPGGHGALLENLND
                     LLADIIFIKNIDNVVPDSFKEITVKYKKIIAGVLIDIQQLCFDYLKELENNNLTENRL
                     KEILRFTEETLCIRHPEIDSFDNETLKKYLFRKLNRPVRVCGMVKNTGEPGGGPFLTV
                     NADGTISPQILESSQIDLNNPEEKQKLENSTHFNPVDLVCSVKNYKGEKFDLTKYVDK
                     NTGFISIKSKNGRELKALELPGLWNGSMSDWNTVFVEVPIETFNPVKTVNDLLRPQHQ
                     "
BASE COUNT         4075 a         2363 c         2333 g         3940 t
ORIGIN      
        1 attttttaca ggaaatattt ttaaaatttc ttttaaattc tatcgttaca ctgtttttac
       61 tctttctaca taagaaccat cacgggtatc gactttaatt aattctcctt cgttaataaa
      121 aagtggaacc cgaattacgg cacctgtttc cactgtggcc ggcttggtgg cattggttgc
      181 ggtatcgcct ttcaaacccg gttcggtata ggtaacttgc aaggtaacgt gagtgggaag
      241 atcaacataa aggattgttt cgctttctgc atgggttacc acgtcacaaa tcattccttc
      301 tttcaaaaaa tctactccat tgattaattc cttatttacg gaaacttgtt caaatgtttc
      361 gttattcata aaattgaatc ccatatcgtc ctgataaaga tattgatagg gacggcgttc
      421 tatacgtact tcatcgattt ttactcccgc gttgaaagtc ttatctattg ttctgcctgt
      481 aactacattt ttcaattttg tacgtacaaa agcagctcct ttgccgggtt taacgtggag
      541 aaattccgta atgatataat actgtccatc aagttcgatg cacatgccgt ttttaatgtc
      601 tgcagtagta gccatagatt tttttattca aaggtaaatg aatgatttta tatattttga
      661 agataaccga tctgtttata aaaaaggttt gcaaatttac tacttttctc ttaattttca
      721 aaatgcttat aaaacactca ttgtcagata aataaaaaaa gagctgctca ttaaaacttg
      781 aacagccctt cgttaattta ttgatgaatt ttatcaaaat tgtctgtcag ccatttgttt
      841 atacatttta taacgccatt tagcattgtt ttcggctgca acaaacaatt cctgagcttc
      901 ggcagggaac tgtttcatca aggaagtata acgaatttcg cctttcagga aatcttggaa
      961 tttttcccat tgcggttctt ttgaatcgag cacaaaagga ttttttcctt ctgcttccaa
     1021 atcgggattg taacgataca attgccaata accgcattca acagcgcgtt cctgttcctc
     1081 ctgagctttt tccatcccgt tatgaatgcc gtgactgata catggtgaat aagcaatgat
     1141 cagcgaaggc ccattgtaag cttctgcttc gcgaattgct ttgagcgttt gtgccttgct
     1201 ggctcccata gcaatttgag ctacatacac ataaccataa gttatggcca tcatacccaa
     1261 atcttttttg cggatttttt ttccggcaga agcaaatttt gctatcgcac caatgggcgt
     1321 tgattttgac gattgtccac cagtattgga ataaacttct gtatccaaaa ccagaatatt
     1381 gacattttta ccggatgcta tcacatgatc caatcctcca taaccgatgt cgtaagccca
     1441 accatcacca ccaataatcc attgcgattt tttaacgaga taatgggaaa gttccgaaat
     1501 tttccgacaa tgttcacaat cacaattttt gagaagtggc gtgattttag cagccaattc
     1561 tttggttttt tcagcgtctt ctttgttttc aatccattcg gcaaaaagtg ctttcagctc
     1621 atcggaacag caattgcttt ctattgcttc tttcatgatt ctttcgagtc tgtcgcgcat
     1681 tacttcggtg gccaattcca ttccgtaacc gaattcggca ttatcttcaa acaacgaatt
     1741 tgcccacgct ggcccttgtc cattttcgtt ggtagtataa ggcgttgatg gagcagaagc
     1801 agaataaatg gaactacaac ccgttgcatt ggctaccaat tctctgtcgc caaagagctg
     1861 agttattaat tttacgtaag gcgtttctcc acaaccggca cacgctcctg aaaattcaaa
     1921 caatggcgtt gccaactgcg aattcttcac attcattttt acatccacca aatgctgttt
     1981 ggatttcact tccgagatac agaaatccca atttttctga ttgtcatatt gggtttctat
     2041 cggtaccatt gtaagcgcct tattgccttt tgttcctgga caaatatcgg cacaattttt
     2101 acagcccata cagtcgagaa catcaacttg aatgcggtaa gtcatgccca caaactgttt
     2161 cccgttagct tttatcgtat cggtgaaagg cgctttggcc tgttcggctt cgtccaaaac
     2221 aaaaggtcga atagcagcgt gaggacaaac gaatgcacac tcgttacact gaatacagtt
     2281 cgcagaattc caaacgggaa cgaaactagc tacgcctcgt ttttcatatt ttgcagttcc
     2341 ggaatcccaa gttccatctt cgcgtccctt gaaaacggag acaggcagat catcgccggc
     2401 ctgagcattt attggcatta ccacttcacg gatgaaagga ggaaagtttt cttccatttc
     2461 cttttcatct tccagatttg cccagttgct tggaacttca actttaacat attctcctcc
     2521 gcgatcaaca gcggcatagt tcatgtttac tatgtcttca ccttttttgc cgtaagtctt
     2581 gttaatagca tatttcattt tttcaactgc caattcgtaa ggaataacat ttgaaatttt
     2641 gaaaaatgct gattgaagaa tagtattggt tctgtttccc aatccaattt cctgagctat
     2701 tttggtagca ttgataatgt aaaaattaat gttgtgtgcg gccagatatt ttttaacttt
     2761 taccggaaga ttttttttca cttcttcttc gtcccaaatt gtattgagca ggaaagttcc
     2821 gtttttcttc aaacctttgg taacatcgta aagtctgaga taagcctgca catggcaagc
     2881 aacaaaatcg ggagtcgtta ccagataagt ggaacgaatt ggttcgtcgc caaagcgaag
     2941 atgagagcaa gtgaaaccac ctgatttttt agaatcgtaa gaaaaatagg cttgacaata
     3001 tttatcggta ttatctccaa ttattttaat tgaatttttg tttgcaccca ctgttccatc
     3061 cgaaccgata ccgtaaaatt ttgcttcaaa tattcctttt ccacccattg gaatttcttc
     3121 ccttagcgga agagaagtaa aggtaacatc atccactatg ccgacggtaa aatgattttt
     3181 tggttcggga agagccaaat tttcaaaaac ggccaaaacc tgagccggcg tgaaatcttt
     3241 ggaagaaagt ccgtatctac cacccacaac ggttggttga ttggatttag catacaatac
     3301 atcctttaca tcaagataaa gaggttcgcc ggaagctccc ggttctttgg tgcgatccaa
     3361 cacgcaaata cgttttgctg tttcgggtaa taccgacaga aaatgttttg ccgagaaagg
     3421 acgataaaga tgtacagaaa tcagtccgac tttttcgcct tttgcgttga gatagtccac
     3481 tccttctttg atggcttcag ttgccgatcc cattgcgata acaactctat cggcatccgg
     3541 agcgccataa taatcaaaca annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 ntgaatttcg tgtgaagtac ggaatccgtc aaaaaagtga acaaaaggaa cgcgtgattt
     3721 gatagccgat aaatgagcca cagcagccat atccataatt tcctgaaccg aacctgttgc
     3781 aaacatggcg cagccggttt gtcgaactgc cattacatcc tgatgatcgc cgaaaattga
     3841 taaagcctgt gcggcaatag aacgagccga aacatgaaaa acgcccggca acagttctcc
     3901 ggaaattttg tacatattgg gaatcatcag cagcaatcct tgcgatgcag tgaaagtagt
     3961 agccaaagct cctgcctgca aagctccgtg cattgctccg gcagctccgg cttccgattg
     4021 catttcctgt acttgaagtt tctcgttgaa aatatttttt cttccgtttg ctgcccattc
     4081 atcaacatat tctgccatag ttgaagaagg tgtgatggga taaatggctg ccacttcgct
     4141 aaacatataa gcgatatggg cggctgcctg atttccatca caagttatga attttttctg
     4201 ttttgccatt gtgttgtgat ttatttgggt ttttactata gtttattatt tggtttagta
     4261 taaaaaaccg aaaagccaaa ttggaatttc attctttttc ccaattttca gttgatcggt
     4321 tgcaaaaatt aattttgaat gttgtttgga aatatttgaa tttaactcgc atttaaacgt
     4381 atattgttta tcaacagtaa aatcggaatg atattttccg gagttaactt tgtattttgc
     4441 atgcagcgaa ttgtacaaaa aggtttttat ttctgataat ctgtcgatat ttcccggcat
     4501 agcggcatgc attaagtttg tattgtgaac atatatcaaa tgaggttttt taggaaattg
     4561 ttcactgtcg ttataaagca agttcaaaag tcgggcatct ttgagatact ttatgtaatt
     4621 cataatagtt gctctcgaag tttgaatttc gcaactcaac tggcttatgt tcggagttgc
     4681 aggggcactg atcatgagta aataaagcaa ttttctgatt tttgaaagat atttcaattc
     4741 gatttgtttg atcagaagaa tatcaacttc aatcatcata ttcatggttt tcagcagatt
     4801 ttccgaaaaa tttctctgtt ccaaaaaaaa cggataataa ccatgatgca gataatcttg
     4861 aaaataaagc aaaggatcaa ttttactgca aatttcattt gctatcggca catgattttt
     4921 tataatatct tccaaatcaa taggttgaaa gtttgtcccg gcttgcaaat tcaaaaattc
     4981 cctaaaggaa aaacctctta gattatatac ttttacaacg tcttgtaaat cttcattgtc
     5041 ttcttttaaa cgcatgactg acgatccgct gaaaattatt ttcagttcgg ggaaacgttc
     5101 ataacattca cgcaattcct ttgaccaatt ttcgtatttg aaagtttgat caatcaacaa
     5161 agtacgtccg ccctgtttca caaaacgctc tgcaaattct accaaagtat gggtagtaaa
     5221 gtaaaaattg ttgaaattga tatacaaaca actttgatca ttcgacctga aaaaatcttt
     5281 tgcatattgc agcaaaaaag tagtctttcc aactcctcta cttcccttta tccctatcaa
     5341 ccgatcggtc cagtctattt catccatcaa agttcgtctc acaggagatt gtacatgttc
     5401 aaccaaatat tgatgcgttc tgaaaaatga ctccatcacg tataaatttt gtttgcaaaa
     5461 atagcaaaat ttatctgatt ttgcaaagtg gagatgacaa ttttagcctt tttattttta
     5521 ttggttaagt tttgggttaa gaaaacttta ataaaattta tttttttagt tggttagggt
     5581 ttaaatttat aattgttgat aaaaaattgg acagaaattt ctgtcaataa caataatcat
     5641 ttacttttga aaaataaaag gctctttttt atcaacagaa attttttaag taaacttaaa
     5701 aaattccaaa aaaaggatta aaaaccaaag attaaaatca ttaattatat gaaaatcaag
     5761 tttataggag cagcacgtga ggttacaggc agcaaacatt taataaccac aaaggatgga
     5821 aaacggatat tgctcgattg tggaatgttt cagggaaaag gattggaaac cgatgccaaa
     5881 aatagagatt tgggtttcga tcccgaaaca atagatcata ttattctttc acatgctcat
     5941 attgatcatt ccggactgat tccctatctc tatcgaaatg gttttcgtgg gtcggtcatt
     6001 tgtaccaatg caacccgtga tctttgttcc attatgttag ccgatagcgc tcatattcag
     6061 gagcttgata ttaaatggtt taataaaaaa cgcgcacgtc agggtttgcc gccagtggag
     6121 cccatttatg ataaggaaga tgcggaaaat tgtatggatt tgtttattgg agtttcactt
     6181 aatcgccgtt tttatattga caataaaatt aatgtgaaat ttaccaatac cggacattta
     6241 ttgggaagtg cagttgtgaa tctcgaaata attgaaaatg gaaaaaaagt tcacattgcc
     6301 tataccgggg atattggtcg ttcaaccgat tacttgttgc gttcaccgga tccctttcct
     6361 caatgtgatt acctgattgc cgaatcaact tatggcaacc gtcttcatcc tgaaacaaat
     6421 caaacggaag aagattttct taaaattata actgaaacct gtatcaaaaa taagggaaaa
     6481 ttgatcattc cagccttttc tattggacga acccaggaat tggtatttct gttgaataat
     6541 ttttataatc gacatttact tcctcacatt gaagtttttg ttgacagtcc tctatctgtg
     6601 aatgctacag aaatttatcg aatgtacagc gaactaatga ataagaatgt gaaagaagtg
     6661 ctcaaatacg acaacgaccc atttggattc aattccttac actacataaa aaatgtggaa
     6721 gaatcaaaag ctttaaacac ctatgacaaa ccctgtataa ttatttctgc ctcgggtatg
     6781 atggaagcag gacgtatcaa acatcacgtg gccaataata ttgaaaatcc gcgaaatacg
     6841 atcttaattg taggttattg ttcgcccact tctttaggtg ctcgcattca gcaacctggc
     6901 ttaagggaaa tatcgatatt tggagaaatt catcccgtta atgccaaaat ttgcaaaatt
     6961 gaggcatttt caggtcatgg cgattataaa gaaatgattg attatctggg ttgccaatca
     7021 gcaggcttga ttaaaaaaac ttttctagtg cacggggatt atgaagttca ggtagaatat
     7081 cgtgaaaaat tacaaaaaga aggtttcagg aaagttgaga tacctgatgc aaaggaggaa
     7141 tttatactgt aggaaaattt ttaaagttga aaacctaaca agttaactta atccaaatta
     7201 attgactatc ataaatttaa gtggctatga agccactaca aatactcctt taattgttaa
     7261 atgtgcctgt ttattagttc ttctaagttt ttatagaaat gtttaaatag atgaattaac
     7321 aaaatgaatt aacagggaaa acataaatat ttgaaacaca tttttttaac caccttaaat
     7381 taaaaattca attttttccg tgaaattttc aaaaaaagtt tcttcatatt aaaagaatat
     7441 cctaaattta gagcgcaata tatcaaagta aaaatttata aacatctaat acttaattgt
     7501 catgacaaaa atgataacat tcaacgaatt gcgtagaatt aaagatgcgc ttccacatgg
     7561 gagcctgagt cagatcgccg aagaacttgg gttggatatt gaaacagtac gaaattactt
     7621 tggcggtcat aattacaaag atggcaaaag ttgcggaatt catattgaac cgggccctga
     7681 tggcggttta gtcatgttgg ataacacaga tattcttgag gctgctttac gaattttggg
     7741 gaaagaagaa gcttaaaagt gcaaaaggaa aaggaaaaaa gaagaaaaag aaattgaaag
     7801 gcagtgaacc agcattcgct gctttttttt aatccgatgc ttaaaaaatc attcgtccaa
     7861 aaatcgtccc attatctata atgtattcat ttttcatcat tttaagcaat atttcctata
     7921 tcattataaa actccctgtc ttattttcac aaaaaagact aattttgcaa catacaacca
     7981 acaatttcat ttcgcatgaa tctttcgccg gcttttgaaa aaagaatgca agttttattg
     8041 gaagaggaat acgctttgtt cagagcagca ttagaaaaac cggctccggt aagtatcaga
     8101 gcaaacaaca aaattgttta ccaaccttcc aaagaacaag tgtcatggtg tgaaagtgga
     8161 ttttatctga gcgaacgccc ggtttttaca attgatccgt tttttcatgc aggcgtatat
     8221 tacgttcagg aagccagttc tatgtttctc cgtcagtgcc tgaaacaata tttttcacag
     8281 gcagaaacag ttttggattt atgtgctgct ccgggcggaa aaacaacgct tctttcacaa
     8341 tatttaaatg aaaatgctct gttaatcagc aacgaaatta atcgttcgcg agcttatatt
     8401 ctggccgaaa atgttgcaaa atggggaaat gccaatgtgg tggttaccaa taatcaaccc
     8461 gctgatttta aaaatcttcc cggctttttc gacgccatgt taatagatgc accctgttcg
     8521 ggagaaggaa tgttcagaaa aaatcccgct tcgattgatg aatggtcgtt gacaaatgtt
     8581 gaaaattgcg tcgttcgaca acggaaaatt atctccgaag tctgggatac tttaaaaacc
     8641 gacggcattc ttgtttatag cacctgtact tacaaccgtc aggaaaacga agcaaacatc
     8701 aaatggattt gcgaagaact cggcgctgaa attttgagaa tggatattca acattttcaa
     8761 ggcattctgg aaacggaagc cggttatcgt ttttttccac ataaaataaa gggggaaggt
     8821 tttttcattt ccgttctacg caaaacttct cctacaccgc aaaaaatgag gaaagcaaaa
     8881 aaaattgaaa aaaagtcgga tgacaaaata aagtccgatg tcggaataaa acttcttgaa
     8941 ccggaaaaat ggaatttgtt gatggaaaac aatcgtctcc gcgcagtaag caaagccagg
     9001 caaaaagaaa ttgctatcat tgaaaataat ttaaaatgcc tgctttcagg tatttatttg
     9061 ggcgattata aaaattccga ttttatccct tctgctcagt ctgctttatc caaagaaacc
     9121 gatttgaaag cagtcgaaac ggttgaaatt gactatacaa ctgccgttcg ttatttaaag
     9181 aaagaaaaca ttcaagttga atctgacaaa aaaggttttt tattgcttct ttataaaggt
     9241 gttcctttgg gatgggttaa aaatatagga cagcgatgca acaaccttta tcctcaggaa
     9301 tggagaataa gaatgaaaat tgaagaatta gaagaaatat gaaaattttg ttattaggca
     9361 ccggcacttc tacaggcgta cctcaaattg gttgcaactg ctatacctgc acttccacca
     9421 acattaaaga tcaacggctg cgttcttcct tgctggtaag cgaaaaaaat accagaattt
     9481 tgattgactg cgggcccgat ttacgacagc aattattgaa ttatcgcatt cacgatttaa
     9541 ccggtatttt gcttacccac gaacattacg accacattgg cggacttgac gatcttcgtc
     9601 cgcttggtcc ttcgcatttg tttggcgaaa aaagagtttt gaaagttatt gaacgaaata
     9661 tgccttactg ttttggcgat aaacgttatc cgggtgtccc cgaattgttt cttcatgaaa
     9721 ttaacgaaga agaattttca ctcgatggtt tacaggttca acctgttcgt gtcatgcatg
     9781 ccaatttgcc tatttttgga ttccgaatcg ggaaaatggc ttaccttacc gacgtcaaaa
     9841 cgctggatga aaaagctgtt gaacaacttc aacaattgga tgttttaata ataaatgcct
     9901 tgcgaccttt taaacatatt tctcatttat ctttgtcgga agccatcgaa ttggcagaaa
     9961 aaattaatgc caaagaaact ttttttaccc atatcagcca cgatatggga cgacataacg
    10021 aaataaattc ggtacttccg ccccatattc atttggctta cgatggattg gagcttgcaa
    10081 tttgagacag agagaaattc agagaaatta ttaaaattat cttctcgcaa actaaatgcg
    10141 cacattttcg tttgatttat gaaaaaaata attaaagcca gatgaaaaaa agttttataa
    10201 tatttgttgg tttatccatt ggggccatgt tgtttagttc ctgttcaaac atgctctata
    10261 cctcgctcga cgtactcaga ccggctaaag ttgctttttt gcccaatgtt aaccggcttt
    10321 tgatagtaaa caacacacct gtcatggcat tggaaaacga tccgaacaaa accaagaaaa
    10381 atgttacgct tccagccgac agtttgccaa tttactgctt aagcgtattg gctcaggaac
    10441 ttggtaacaa cagctttttt tcatccgttc tgttggaacc taactccatt tatccaaaaa
    10501 acaattattc cgaaaagctt tcaaccgaaa tggtaaaaaa actttgtgaa aattacaact
    10561 gtgatgtaat tctgacattg gacagtctca aagcttcgga tgtgttgaca gaatattatc
    10621 tcgaagacat tagccagtat ttaagtacgt atgaaattta cctcaaatca aaatggagta
    10681 tttactatcc ttcgcaatcc aaaccggttt ctttacagtt taaagataca gtatattggg
    10741 aagctcagga ttattcgcgg cagagagctt tgaagcaaat gcccgacaga tgggatgcat
    10801 tggttgatgg cgctttgtat gtaggaaaaa attcggtaaa aagatttatt ccttattggg
    10861 ataaagttga tcgttatttt ttcaatcccg gcaacaagtt aatgcgacag gcaatggatt
    10921 ctgtttatgt aagaaattgg acttcggcca ttgaattgtg gaaaaaagcc atgaacaaat
    10981 ccaaaagcga aaaactcaaa ggtgaagctg ccaacaatat tgccatcgcc tatgaaattc
    11041 ttggcgatat ggataatgcc attgcgtatg ccagacagtc tcttcaatat ttttcttctg
    11101 tttccattat tgattatgac acaatggaaa gaatcaacaa ctattactac gaactgataa
    11161 aacgaaaaaa tgaaattcaa caactgaaat tgcagttggg agaatgattt ttttgagcaa
    11221 tggagcaaca atcataaaaa tcaattcaac ttattgataa ccaacaaaaa acgaaaaaat
    11281 atggaactac ttttgccttc tgatgaaatt ttattacagg aaaaaggaat cggcaaacag
    11341 caaatcgaag aacaattgca ttattttgaa acaggattcc cttttttgaa aattgttgca
    11401 ccggcagtaa ttggcaaggg aattacaatt atcgatgata aaaaaggaga attttacatt
    11461 aacaaatggg aaagttacag acaaaaagaa ggaatcgatg tggttaaatt tgttcctgcc
    11521 tctggagcgg catcccgaat gtttaaagat ttgtacgaat ttttggaaag tgaagcagaa
    11581 ttacctcaaa aatcttctgt aaaagatttt ttcaatcaaa ttgaaaaatt tgccttttac
    11641 gaagagttaa acaaagcctg cctgaaaaat gagggaaaaa gtgttgccga attgataaaa
    11701 aacaacagac aaaaaacggt agtagaaaat ctgttgcttg aaaaaggttt gaattacggc
    11761 tttctaccga aaggtttgtt gctttttcat tcctatcccg acgaaaaaag gactccggtt
    11821 caggaacatt tggttgaagg agctttgtat gctacccatg ccgacaacga tgtaaaaatc
    11881 cattttacag tttcacatca acacagagct ttatttgaaa aacacattca ggaaattctg
    11941 gaaaaatatg agcaaaaatt caaagtacat tatcatatcg acttttctga acaaaaaccc
    12001 agtaccgaca cattggctgt agatatgaac aatcggcctt tcagagaaaa cgggcaactg
    12061 gtatttcgtc cgggaggtca tggcgcttta ctggaaaacc tgaacgacct acttgccgat
    12121 atcattttca taaaaaacat cgacaatgta gtgccggatt cctttaaaga aattaccgtc
    12181 aaatacaaaa aaattatagc cggagtttta attgatattc aacaactgtg cttcgattat
    12241 ttaaaagaac tggaaaacaa taatttaacc gaaaatcggt tgaaagaaat acttcgattc
    12301 acagaagaaa ctctttgtat tcgtcatccc gaaatcgatt cttttgacaa cgaaacatta
    12361 aaaaaatatc tgttccgtaa attaaatcga cctgtgaggg tctgcggaat ggtaaaaaac
    12421 acgggagaac ccggcggtgg tccattcctc accgtaaatg ccgacggaac gatttctccg
    12481 caaattttgg aaagctcaca aatcgacctg aacaatccgg aagagaaaca aaagttggaa
    12541 aattctacac atttcaatcc ggtagatttg gtttgctcgg taaaaaatta caagggagaa
    12601 aaatttgatt taacgaaata tgtcgataaa aataccggtt ttatctccat taagtcaaaa
    12661 aacggaaggg aactgaaagc gctggaactt ccgggtttgt ggaacggttc aatgagtgat
    12721 tggaatactg tttttgtaga agttccgatt gaaactttta atccggtgaa aaccgtcaac
    12781 gatttgctac gccctcaaca tcaataaaaa a
//
LOCUS       DAOSIB010000063        12657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_5014_length_12657_cov_11.127519, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000063 DAOSIB010000000
VERSION     DAOSIB010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12657
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5014_length_12657_cov_11.127519"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..606
                     /locus_tag="PLD44_06475"
     CDS             139..606
                     /locus_tag="PLD44_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4494 domain-containing protein"
                     /protein_id="HOF99014.1"
                     /translation="MHTWFECKIKYEKVIEEGKTLTVNETYLIDALSFTEAEKRIIQE
                     MEPYISGDFLVANIRRARINEIFAHDGGDRWYRCKVFFISLDEEKSVEKRTAVTMLVQ
                     ATSVKEAIEVLTSEMKNSLVDYELVSVSETPIMEVYPYVAEEKKVVQETSNFQ"
     gene            635..1315
                     /locus_tag="PLD44_06480"
     CDS             635..1315
                     /locus_tag="PLD44_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOF99015.1"
                     /translation="MSIEKNLKKIRVQIPENVQLVCVSKFQPNKAILEAYHLGERIFG
                     ESRVQELCEKQKSLPGDIQWHFIGHLQTNKVKYIVPFVDLIHAVDSFRLLEEIDRNAR
                     KIGRVVRCLLEIHIARESSKFGFLPEQLIEMLKGEGWKNLHNVQICGLMGMATYTDNQ
                     TQIRQEFRNLKLLYDQLKDSFFSNEASFRELSIGMSNDFQIAIEEGSTIVRLGTCIFG
                     ERNCTVIT"
     gene            1689..2384
                     /locus_tag="PLD44_06485"
     CDS             1689..2384
                     /locus_tag="PLD44_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOF99016.1"
                     /translation="MNKIVVLTGAGMSAESGLSTFRDSGGLWEQYRIEEVATPEAFKK
                     NPEKVLNFYNERRKQLLKTLPNEGHEALVMLENFFDVYIITQNVDNLHEKAGSSRVIH
                     LHGELMKVCSTGPGHEVYELSSENIEIHVGDKCPKGYQLRPHVVWFGEPVPEIEKAIN
                     LVSQADIFIIIGTSMQVYPAAGLIRYVKPGTPIYLIDPKEVMVNTSNTTFIKKGASEG
                     IKELVQILKNTYL"
     gene            complement(2502..2798)
                     /locus_tag="PLD44_06490"
     CDS             complement(2502..2798)
                     /locus_tag="PLD44_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99017.1"
                     /translation="MKAIFIVFDQGFYQQILESLYINHHVKGFTAWETVKGRGSKTGE
                     PHYGSHAWPSLNSAIITVVAEEKVKPILDDLKALDATSDVMGIRAFVWNIEDGM"
     gene            complement(2978..6109)
                     /locus_tag="PLD44_06495"
     CDS             complement(2978..6109)
                     /locus_tag="PLD44_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOF99018.1"
                     /translation="MSLYENSVKKPIMTSLIFVGVIILGLYSLSRLPIDLLPDIEPNI
                     LMVATSYTGASALDIENNVTRPLENTLNTVENLKNIRSNSKENSSVIILEFEFGYDLD
                     VLANDVRDKLELVKPYLPEGASNPVIFKFSTDMIPIVMISATADKSLPALYKILDENV
                     ANPLARINGVGSVSINGAPEREIQVYVDPVKLEAYGLTVESIGQNIRLQNINTPSGSM
                     DIGNKTYTVRVQGEFKDPSELSKVVVGSFNGKNVYLSDVAIINDGHEERVQESYTNGV
                     QGATIIIQKQSGANTVEIANAIRAKLPQIEKNLPSDIKLNIINDSSENIKNTIAGLEE
                     TILYAFLFVILVVLFFLGRWRATIIVILTIPISLIAAFIYLSGTGGTLNIISLSSLSI
                     AIGMVVDDAIVVLENITKHIEKGSRPNSAAIHGTNEVATAVVASTLTIVAVFFPLTLV
                     GGLAGILFAQLGWMVTIIMVVSLVCALSLTPMLSSKMLRLNPHHSRIFTVVYSPIVKF
                     LDNVDAWYANIVNWSVRHRWQTVAIAAGFFVLSLIPLIGIGTEYIPAQDNSRISATIE
                     LPVGTRMEISKKTAENLTELWREKYPEIQTINYSVGQASSSNIIGSIQNTGSHIISFT
                     LRLSDTNERNRTIFEIADSMRVDLKSFLEINKANVTAGGAMGTMGGQSTLDIEIYGYD
                     FNETDKVAMELTNRINNIQGLVNTTISRGDYSPEYQVEFDRDKLALNGLNIGTASTFL
                     RNRINGLTASLYREQGDEYTIRVVYAPEYRQSIEDVENILIYNTMGQAIRLKDLGKVI
                     ETLTPPYIERKNRERVITVSSTISGTTLDKAVQAINTELKNMEIPTGVDTQISGSYED
                     QQESFADLTTLLLIIIILVYIVMASQFESLTYPFIIMLALPFAFSGVVLALFLTGGTL
                     NLMSMIGIIMLVGIVVKNGIVLVDYINLNRERGMGIVRAIVSGGRSRLRPVLMTTFTT
                     ILGMIPLAISSSQGSEIWKPMAITIIGGLTVSTILTLLIVPTLYAIFAGFGIKRTYNK
                     YKKGTNKLQQSEK"
     gene            complement(6177..7193)
                     /locus_tag="PLD44_06500"
     CDS             complement(6177..7193)
                     /locus_tag="PLD44_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOF99019.1"
                     /translation="MKRINKLGILIMALTIGLVSCGKSGKKQQVEEEIPTVRVQQVKL
                     QDVEQTGQFTATVQPEVKNNISPSTPGRIRKIMVEVGTSVSKGQKLVQMDAANLSNLE
                     IQVENYQRMYNRVAELFSVGGASQQELDNAKLQLDIAKTNLQNLSENTYLLSPTAGVV
                     TARNYDNGDIFNGQIPILTVMQINPVKLLINVSESNYAQVKIGMPATIKFDIFEGETF
                     QGKISLIYPTIDERTRSFQVEIKMNNANYKIRPGMFARVEINFGSARRIIVPDMAVIK
                     QSGSGEYFVYVYKDGSVIYQRVELGKRLGKEYEIISGLNDGDLVVVSSQSKLADGKKV
                     NLQK"
     gene            complement(7251..8603)
                     /locus_tag="PLD44_06505"
     CDS             complement(7251..8603)
                     /locus_tag="PLD44_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOF99020.1"
                     /translation="MTANSLRKSIFIVLCVIALLPFSLKAQDTLIVSLDKAIEIALSE
                     SPTIKVANKEIQRVDYSSKEKISALFPVISASGQYSRAVKKQKLFFNIPGMPARPDGI
                     EVGQDNTFSGGISAAMPLIAPTLWATIKMSELDAQLVMESARSSKLSLVNEVSKAYYA
                     ILLAQDSYDVFKKSYDIAVENAKIIENKYKQGTVSEFEWIRADVQVKNANANLVSAEN
                     AVNLSVLRLKMLMGIDMFIGLKVEGNLSNYEANKYADVLSIDTTTLKSNSDLKQFDIK
                     AQQLKQSLKIQKSTNLPTLSAAINYQYMSMADDSIAFSNYHWFPTSTAALSLSIPLFQ
                     GGGKLFKQKQIEIQVEEMKYQRDNLRRGLELQAMSYLDNINQSLKVIESNKEGLRQAE
                     KALSISQKRYEVGAATYLDLSNAELAYTQAGLSYNQSIYNYLSAKADLEKLLGNQIIF
                     "
     gene            complement(8641..9252)
                     /locus_tag="PLD44_06510"
     CDS             complement(8641..9252)
                     /locus_tag="PLD44_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOF99021.1"
                     /translation="MKNTEEVILQKSLDLFIEFGIRNVSIDTICNELRISKKTFYSYF
                     KQKEELVDAILTYEEKINIEEMKKRFDDKNAIESLISVLKEIKKNIDCQPKLMWMDIK
                     KYYPKVYEKHEMRRRSIIRKDFEKNLQQGVKEGFYRDDLDIELLSMLHSYQLKNIMTT
                     MSQYPKKYSKKRLVDFFIDIMIHIIANEKGLEYVRKNYYESEP"
     gene            complement(10121..11692)
                     /locus_tag="PLD44_06515"
     CDS             complement(10121..11692)
                     /locus_tag="PLD44_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF99022.1"
                     /translation="MHKEIQITLPPEKVNNKSVLEKEIIKIINIEIQQITRWRIIRKS
                     IDARSYLPKFNLSVEVYWDEIPPEDTAYNFHYNFVGNKPSVLIIGSGPAGLFAALKLI
                     ELGLKPVILERGKQINQRKKDIASICRNEGIDPNSNYCFGEGGAGTFSDGKLYTRSKK
                     RGDKLHVLQVFHHHGANENILYEAQPHIGSDKLPLIVENICKTIIDCGGEIHFETRIT
                     DILLKNGHLTGCKTTDNNIFEAASLILATGHSARDVYEMLYHQGILLENKGFAMGVRV
                     EHPQQLIDSIQYHKKYRGDFLPPANYQLVEQVEGRGVYSFCMCPGGHIVPAGSGTKEI
                     VVNGMSASKRNSPFANSGIVVEIKPEDIPSDFSGCGPLASLMFQKHLEKLAYINNGGL
                     NLTAPAQRLTDFVNGKLSDNLPTCSYLPGLISSPLHFWMPDFLTKRLQEGFKKFDKKM
                     HGFLTREAVIVGVESRSSSPIRIPRDKETYQHPQIKGLFPCGEGSGYAGGITSSALDG
                     ENAAIAVYKSRLYHN"
     gene            12253..12435
                     /locus_tag="PLD44_06520"
     CDS             12253..12435
                     /locus_tag="PLD44_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99023.1"
                     /translation="MHFDLSVRCSPDRKMLESYFRIKENYRDIAGVVRSYILLVPGSI
                     LELDPNQRKQVAIIGN"
BASE COUNT         4023 a         2318 c         2182 g         4134 t
ORIGIN      
        1 gacatgtggg ttccatcaga cctttaaaaa ataaattata ttctgatgaa aatcaaaatt
       61 tatcccttaa atttgtagaa actgcaacag taagcaattt aataactaaa tttattcata
      121 tttaaacaat tagttaagat gcacacttgg ttcgaatgca aaatcaaata cgaaaaagtg
      181 attgaagaag gaaaaaccct taccgtgaac gaaacatact taatagatgc tttaagtttt
      241 acggaagctg aaaagcggat tattcaggaa atggaacctt atatatctgg agattttttg
      301 gtggccaaca ttcgcagagc gcgcattaac gaaatttttg cacatgatgg cggtgacaga
      361 tggtaccgtt gcaaagtttt ctttatttca ttggatgaag aaaaaagtgt agaaaagcgt
      421 acggctgtta ctatgcttgt tcaggctact tcagtaaaag aagctataga agttcttact
      481 tcagaaatga aaaattcttt ggtcgattat gaattagttt ccgtttccga aacgcccata
      541 atggaagttt atccttatgt ggcggaagag aaaaaagttg ttcaagaaac ttcgaatttc
      601 caataaggaa aaatttacaa aagaatacat aaacatgtct atcgaaaaga atttaaaaaa
      661 aattcgtgtt caaattcccg aaaacgtgca attggtatgt gtttccaagt ttcaacccaa
      721 caaagccata ctcgaagcat atcacttggg tgaaagaatt tttggagaaa gcagggtaca
      781 ggaactttgt gaaaaacaaa agtctcttcc aggtgatatt caatggcatt ttataggaca
      841 tcttcaaacc aacaaggtga agtatattgt tccgtttgtg gatttgattc atgccgtaga
      901 ttctttcaga cttttggaag aaattgaccg taacgcccgt aaaattggaa gagttgttcg
      961 ttgcttgttg gaaatccata ttgctcgtga atcatctaaa ttcggatttc tacctgaaca
     1021 acttatagaa atgttgaagg gcgaagggtg gaaaaattta cacaatgttc aaatttgtgg
     1081 gttgatgggc atggcaacct atacagataa tcaaactcaa atccgacagg aattcaggaa
     1141 tttaaaatta ctatacgacc aactgaaaga ttcatttttc agtaacgaag catcctttcg
     1201 agaactttca ataggtatgt ctaatgattt ccagattgca atagaagaag gcagtacaat
     1261 cgtcaggttg ggaacttgca tttttggcga aagaaattgt actgttatca cttaaaaaat
     1321 tatggcattc actccgaaaa gctttttaaa attagtttgt ttttaaacag cagtatagag
     1381 tcggtcaaga aaaagctgtg atagcaaggc agaatgaaga acttaatccc caatttgttt
     1441 gtgcgctaca tcagtccgat tgtcgctaca agggcaaaac cgactcaaca aaaaaaggcc
     1501 agaaatggac aatcaaaatc taaaaggcgg atattgttat ttcaatattt agaaacccct
     1561 aatggctgat ttttctgcaa aagttgctgt ttttatacaa attatcgaaa catatcgata
     1621 caataaaatt taacaacttt ttttgataaa aatctcacat ttatctgcaa ttataataca
     1681 gacaaacaat gaacaaaatt gtagttttaa ccggtgcagg catgagcgct gaaagtggct
     1741 tgtctacttt tagagatagc ggaggattat gggaacaata tcgtatagaa gaagttgcta
     1801 cccctgaagc attcaagaaa aatcccgaga aagtattaaa tttctataat gaacgccgga
     1861 aacagttgct taaaaccttg cccaatgaag gacatgaagc tttggtcatg ttggaaaatt
     1921 tttttgatgt gtatattatt actcaaaatg ttgacaattt acacgaaaaa gcaggaagca
     1981 gtcgtgttat tcatctgcat ggagaattaa tgaaagtttg ctccaccggt ccgggacatg
     2041 aagtgtatga attaagttct gaaaatatcg aaattcatgt gggagataaa tgtcctaaag
     2101 gttatcaatt acgaccgcac gtagtttggt ttggtgagcc tgtccccgaa atagaaaaag
     2161 ccataaacct cgtttctcaa gccgatatat ttataattat tggtacttca atgcaagttt
     2221 atcctgctgc aggattaatt cgctacgtca aacccggtac gccaatttat ctcattgatc
     2281 caaaagaagt aatggtaaat acatcaaata caacttttat aaaaaaagga gcaagcgaag
     2341 gaataaaaga gttggtgcaa atactgaaaa atacgtatct gtaatttcat ctcgataaaa
     2401 tcaattttct attattaaaa aggcactcat tattagagcg ccttttcact tattgagcaa
     2461 tgcagtaaaa ataaaaaata aaattcaagc aaaaacaaga attacatacc atcttcaata
     2521 ttccaaacaa atgcacgaat tcccatcaca tccgaagttg catcaagagc ttttagatca
     2581 tccagtatag gttttacttt ttcttcagcc actacagtta taattgccga attcagggat
     2641 ggccatgcat ggcttccata atgaggttcg ccggttttag acccgcgtcc ttttactgtt
     2701 tcccaagcgg taaatccttt aacatgatga tttatataaa gcgattcgag tatctgctga
     2761 taaaaacctt gatcgaaaac tataaatatt gctttcataa atattgtttt ttctgattaa
     2821 caatcgggat tggaccagat atattaaata agaacttaac caaacccagt ccaatctcaa
     2881 tcttcttttt tgaattgtat aaaaacattt tgtttaactt aaaatcttta cctacttttt
     2941 tatattttct tggtcaggta aaaattcaat taaattatta tttttcggat tgttgcaact
     3001 tattggttcc ctttttgtac ttgttgtaag tgcgtttaat tccgaatccg gcaaaaattg
     3061 catacaacgt tggcacaata agtaaagtca gaatagtgga aactgttaag ccaccaataa
     3121 tggttatggc cattggtttc catatctctg atccttgaga tgaacttatt gccaaaggaa
     3181 tcataccaag tatagtagta aaagttgtca tcaatacggg acgcaaacgg gaacgacctc
     3241 cgctgacaat tgccctgaca attcccatgc cgcgttcacg atttaaattg atataatcta
     3301 ccagaacaat tccgtttttt actacaatac cgaccaacat gataattcct atcatggaca
     3361 ttaaatttaa cgtaccaccc gttaaaaaca atgccagcac aactcccgaa aatgcaaatg
     3421 gtaaagcaag cataatgata aacggataag tcaaggactc aaattgtgat gccataacta
     3481 tgtaaaccaa aatgatgata atcagcaaca aagttgttaa atcagcaaat gactcttgtt
     3541 gatcttcata agaaccggaa atctgtgtgt caactcctgt tggtatttcc atattcttaa
     3601 gttctgtgtt gatggcttgc actgctttat cgagagtggt tcccgaaata gttgaggaaa
     3661 ccgtaatcac acgttcacga ttttttcttt ctatataggg aggtgttaag gtttctatca
     3721 ctttccccaa atctttaagc cgtatagctt gtcccatagt attgtatatc aatatgtttt
     3781 caacatcttc tatactctgt ctgtattcgg gagcatatac tacacgtatg gtatattcat
     3841 cgccttgttc acgatacagg ctggctgtta aaccattaat tctgttccgc aaaaaggtgg
     3901 atgcggttcc aatgttcaat ccgttaagtg ccagtttatc tcgatcaaat tctacttgat
     3961 attcgggtga gtaatcacct cgactaattg ttgtattaac cagtccctga atattgttta
     4021 ttctgttagt taattccatt gccactttat cggtttcgtt gaaatcgtag ccatatattt
     4081 ctatatccag cgtcgactgt cctcccattg ttcccattgc tccacctgca gttacatttg
     4141 ctttgtttat ttccaaaaag gatttgagat ccacacgcat actgtctgca atttcaaaaa
     4201 tagttctatt tcgttcgttc gtatccgaca aacgcagggt aaaagaaatg atatggctac
     4261 cggtgttctg aattgaacca atgatgttgg aactgctggc ctgccctacg gaataattaa
     4321 tggtttgaat ttcaggatat ttttctctcc aaagttctgt aagattttct gctgttttct
     4381 tagagatttc catccttgta ccaacaggta gttctatggt ggcactgatg cgcgaattgt
     4441 cctgagcagg aatatattct gttcctatac cgattaaggg aatcaaactc aacacaaaaa
     4501 atcctgctgc gatagcaact gtttgccatc tgtgacgaac cgaccagttg actatattgg
     4561 cataccacgc atccacattg tcgagaaatt ttacaatcgg tgaataaacg acagtaaata
     4621 tacgagaatg gtgaggatta agtcgcagca ttttcgagct caacattgga gtaagcgaca
     4681 aggcacaaac cagcgaaact accataataa tagtaaccat ccagcctaat tgggcaaaaa
     4741 gaatccctgc caatccgcct accagcgtca agggaaagaa aacggcaact atcgtgagag
     4801 ttgaagcaac aacagcagtt gcaacttcgt tggtaccgtg tattgctgcg ctattcggtc
     4861 tacttccttt ttcaatatgc tttgtaatat tttcaagtac cacaatagca tcgtcaacca
     4921 ccatacctat tgcaatcgat aatgaactga gcgaaatgat atttagggtt cctcctgtgc
     4981 cgctcaaata aataaaggca gcgataagtg atataggaat tgtaagaatg acgataatgg
     5041 tcgctctcca tcttcccaag aaaaacaaca ccaccaaaat aacaaagagg aaagcatata
     5101 aaatagtttc ttccagaccg gctatcgtgt ttttaatatt ctctgaactg tcattaataa
     5161 tattcagttt tatatcagaa ggaagatttt tttctatttg gggtaatttg gctctgatgg
     5221 cattggcaat ttccaccgta tttgcaccgc tttgtttttg aattatgata gttgctccct
     5281 gaacgccatt ggtgtaactt tcctgaacac gttcttcgtg tccgtcgttt attattgcca
     5341 catccgacaa atatacattc ttcccattga aacttcccac tacaacttta ctcagctcgc
     5401 tagggtcttt aaattctccc tgtaccctga cagtataggt cttatttcct atatccatcg
     5461 aaccacttgg agtgttgata ttttgaagac ggatattctg accaattgat tcaacagtta
     5521 aaccataagc ttcaagtttg accggatcaa catatacctg aatttccctt tcgggagctc
     5581 cgttgatgga aacagaacca acaccgttga tacgtgccag aggattggct acattttcat
     5641 caagtatttt ataaagtgcc ggtaaacttt tatctgctgt tgcagaaatc atcacaatgg
     5701 gaatcatatc ggtactgaat ttaaaaatta ccggattact ggctccttca ggcaagtagg
     5761 gtttgaccaa ttcgagcttg tctctaacat cattggctaa tacatccaag tcgtatccaa
     5821 attcaaattc gagaataata acagaagagt tttcttttga attggaacga atatttttca
     5881 aattttcaac tgtattcaat gtgttttcaa gcggacgggt aacattgttt tcaatgtcca
     5941 atgcacttgc tcctgtataa ctggttgcta ccattaaaat attgggctct atatcgggaa
     6001 gcaaatcaat aggtagcctg cttaatgaat acaaccccaa aataattact ccaacaaaaa
     6061 tcagagatgt cattatgggc tttttaaccg aattttcgta taaactcata gattttaaat
     6121 tttgaactaa gttattttaa taccgttttt tcaaaaactt ttttcggtaa aaatttttat
     6181 ttttgtaaat tcactttctt accatctgcc aattttgact gacttgaaac cacaaccaag
     6241 tctccatcat tcaggccgga aataatttca tattctttgc ccaagcgttt acctaattcc
     6301 actctctgat aaattaccga tccatcctta tagacataaa caaaatattc tcccgaaccg
     6361 gattgcttaa taacagccat atccggaaca ataattctcc gggcagaacc gaaattgatt
     6421 tcaactcttg caaacatgcc gggacgaatt ttataattag cattattcat tttaatttct
     6481 acttgaaagg atcgagtgcg ttcatcaata gttggataaa tcaaactgat ttttccctga
     6541 aaggtttctc cttcaaaaat gtcgaattta atggtagccg gcattccaat ttttacctgt
     6601 gcataatttg attccgatac attgatcagt agttttaccg gattaatttg cataacggta
     6661 agaatgggga tttgtccgtt aaaaatatca ccattgtcgt agttacgtgc tgtaacaaca
     6721 cctgctgtcg ggctgagcaa atatgtattt tcggataaat tttgcaaatt ggttttagct
     6781 atatccaatt gcaatttggc attgtcgagt tcctgctgtg aagcacctcc gacgctgaaa
     6841 agttctgcca cccgattata cattcgctga taattttcaa cctgaatttc gaggttcgac
     6901 aaattggcag catccatctg aaccagtttt tggccttttg aaacagaagt gccaacttca
     6961 accataattt ttcttatcct gcctggtgtg gagggagaaa tattgttttt aacttccggt
     7021 tgaacagttg ccgtaaattg accggtttgt tctacatcct gcagttttac ttgctgaacc
     7081 cgaacagttg gaatttcttc ttctacttgc tgttttttcc ctgattttcc gcatgacacc
     7141 agcccaattg ttaaggccat aattaatatc cctaatttat ttatcctttt cattttaatg
     7201 tcatttattt ggttgttatt ataaattgaa atctgtgtta tattttaatt ttagaaaata
     7261 atctgattgc cgagtaattt ttccagatcg gcttttgcgg acaagtaatt gtaaatagac
     7321 tgattataag ataagcctgc ttgggtatag gccaattcgg cattggacaa atcgagataa
     7381 gttgcagctc caacttcata acgtttttgt gaaatactca gtgctttctc cgcctgtcgc
     7441 aatccttcct tgttcgattc tatcactttt aaggactgat taatgttatc caaatacgac
     7501 atggcttgta attccaaacc tctcctgaga ttgtcgcgct gatatttcat ttcttctacc
     7561 tgaatctcaa tttgtttttg cttaaagagt tttcctcctc cttgaaacag aggaatagaa
     7621 agagaaagtg cagccgttga agtggggaac caatgataat tggaaaatgc tatcgaatcg
     7681 tcagccatac tcatatactg ataattgata gctgccgata aggtaggaag attagtggat
     7741 ttctgaatct tcaaactttg tttgagttgc tgggctttga tatcgaattg tttcaaatcg
     7801 ctattacttt tcagggtagt ggtgtcaatg gatagcacat ctgcatattt gttggcttca
     7861 tagtttgata gatttccttc aacttttaaa ccgataaaca tgtcaattcc catcaacatt
     7921 tttagtctta aaacactcaa attaactgca ttttctgctg aaaccaaatt ggcattggca
     7981 tttttgacct gcacgtcagc acgaatccat tcaaattcgg atacggttcc ctgtttgtat
     8041 ttgttttcta taatttttgc attttctacc gctatgtcgt aacttttttt aaatacatcg
     8101 tacgagtcct gcgccaacaa aatagcataa taagcttttg aaacttcgtt aacaagtgaa
     8161 agttttgaag aacgtgcaga ttccattacc aactgggcat caagctccga catttttatg
     8221 gttgcccaga gagtaggcgc aattaaaggc atggcagccg aaattcctcc actgaaagta
     8281 ttgtcctgtc ctacttcaat tccgtcagga cgtgccggca taccgggaat attaaaaaac
     8341 aatttctgtt tttttacagc tctggagtat tgccccgaag ccgatatgac aggaaataaa
     8401 gcggaaattt tttccttgct cgagtaatcc actctttgaa tttccttgtt ggcaacctta
     8461 atggtcggac tttcactcaa cgctatttct attgccttat cgagtgaaac aatcaatgta
     8521 tcttgcgctt tgagagagaa aggcaaaagc gctattacac aaagaacaat aaaaattgat
     8581 tttcttaatg aatttgctgt catattcttt atttattttt tggttatacc aatttaaaac
     8641 ttaaggttca ctttcataat agttttttct tacatattca agtccctttt cattggcaat
     8701 aatgtgtatc ataatgtcta tgaaaaagtc aacgagccgt ttttttgaat attttttggg
     8761 gtattggctc atggtggtca ttatgttttt cagttggtat gaatggagca tcgaaagcaa
     8821 ttcaatgtct aaatcatctc gataaaatcc ttcttttact ccttgttgaa ggttcttctc
     8881 aaaatctttt ctgataatag aacgcctgcg catttcgtgc ttttcgtaaa ctttaggata
     8941 gtattttttt atgtccatcc acataagttt aggttggcag tcgatgtttt tcttaatttc
     9001 tttcaaaacg gagataagtg attcaattgc atttttatca tcgaatcttt ttttcatttc
     9061 ttcaatattg attttttctt cataagtcaa tattgcgtcc accaattctt ccttttgttt
     9121 aaaataactg tagaaagtct ttttcgaaat tctcagctca ttgcaaatgg tatcaatgga
     9181 aacatttctg ataccaaatt caataaacaa atccaacgat ttttgaagta taacttcttc
     9241 agtatttttc attttgcgaa ataaaaaatt ttcattgcaa aattaattaa aaaatcatcg
     9301 tacataagca aaaaaacaat aacaaaaagt taaatattat ttttttgtat ataagagact
     9361 gtttatcaat aatttaattg aatgtaatta aaaactttta gaatttaata gtttccaagt
     9421 aaatatctat ataaaaattt gactgtacaa tattgttaaa ataaattatc aaaagcattt
     9481 cattttaaat ttatactttt acgttacagc aaggatggca attcaaaaaa tcatggtaac
     9541 atttaaggat tgaatcattc aattatcaat taagccataa aatggttcag aattttgaat
     9601 gaaaattttc atcaaaaatt aagccaaaac caataggtac ataaaaaaat gggagacaaa
     9661 ataattttta ccatcatttt atttatttca acccgaaatc ctgtggctga agtcggtgtt
     9721 tttttatcag ataataaaaa cacaattgtt ctccaattaa cttcgctcca tttttagtcc
     9781 aaccggcaac aattcgtagt ttatgttcaa tgaaatttcg attatgaaac ccatatgtat
     9841 tacatacaca accacgaatg aatatagtat tcagacatgt ggcttccatc ggaccattaa
     9901 aaaataaatt atattctgtt gaaataaata aataaattcc tttattgctt attaaacgat
     9961 ttttcctatc gtaatttaag gcgtcttaaa ccgaaagata cttttttcta aacataagca
    10021 cacctatatg ttctatttta ttgatattta attaaataga tagaatagaa aaaatttaga
    10081 ggagagctat tcaataattt cagtcgcaaa caaaatggaa ctaattatga tataatctac
    10141 ttttataaac agctatggca gcattttctc catctaaagc tgaggaggta attcctccgg
    10201 catagcctga accttcacca caggggaaaa gcccttttat ttgaggatgc tgataagttt
    10261 ccttatcacg tggtatcctt attggagagg aagaacgcga ttctacacca acaataactg
    10321 cttcacgggt gagaaatccg tgcatttttt tgtcaaattt tttaaatcct tcttgtaaac
    10381 gtttagtcaa aaaatcaggc atccaaaaat gtaacgggga agagatcaaa ccaggaagat
    10441 aggaacaagt aggcaaattg tcggagagtt ttccattcac aaaatccgtt aaccgctgag
    10501 ccggagcagt cagattcaaa ccaccattat tgatataggc caatttttct aaatgttttt
    10561 gaaacataag tgaggcaagt ggcccacaac ctgaaaagtc tgaaggaata tcctcaggtt
    10621 ttatttcgac aactataccc gaattggcaa agggggaatt tcttttggat gcagacattc
    10681 cgttgacaac aatttctttt gttccacttc cggccggcac aatatgtcct cccggacaca
    10741 tacaaaagga atatacgcca cgtccctcca cttgttcaac caactgataa ttggccggag
    10801 ggagaaaatc tcctcgatac tttttatgat actgaatact atcaattaat tgttgggggt
    10861 gttccacacg aacacccatt gcaaaaccct tattttcgag cagaattcct tgatgataaa
    10921 gcatttcata aacatcgcgc gcagaatgac cggtagccaa aatcagggat gcagcttcaa
    10981 aaatattatt atcagttgtt ttacaacctg ttaaatgtcc attttttaat aaaatatcgg
    11041 taattcttgt ttcaaaatga atctctcctc cacaatcaat aatggtttta catatattct
    11101 caacgattaa gggcaatttg tcggaaccaa tgtggggctg tgcttcataa agaatatttt
    11161 catttgcgcc atgatgatga aaaacctgta aaacatgaag tttatctcct cttttttttg
    11221 agcgagtgta aagcttacca tcagaaaagg ttccggcacc tccttctccg aaacaataat
    11281 tggaatttgg atcaataccc tcatttcgac aaatggaagc aatatctttt tttctttgat
    11341 ttatttgctt gcctctttcc aatataacag gtttaaggcc aagttctata agttttaagg
    11401 ctgcaaaaag ccctgccggc cccgaaccga taattaagac cgatggcttg ttgccaacaa
    11461 aattataatg aaaattatag gctgtgtctt ccggtggaat ttcgtcccaa taaacttcaa
    11521 cagacaagtt gaattttggt agataagaac gggcatcaat ggatttgcga atgattctcc
    11581 atcttgtaat ctgctgtatc tctatattta taattttgat tatctctttt tcgagtactg
    11641 atttattatt tactttttcc ggtggtaaag taatttggat ttccttatgc atatttataa
    11701 agtaaatttt tctttatcac aaaagtatag gattaatctc aaaagaaaaa attatttttt
    11761 tcattgttgt tcggtaaaca tttataaaaa ttagtgaaaa ccattataga aaatgtctta
    11821 ctataatatc gcccctaatg gggcttagat tatgtgggtt gaatttttct accataattt
    11881 cgctgcaatg cagctttctc ttaagtccca tcgggacgat attatggtag tcaataagtt
    11941 acaaatatca ttagagctcc gtaggagcga cactatttgc tcctttattt ttatcggaca
    12001 acattaattt tttttaaaaa aatgcagatt acttgctgtt taacaattga ctatttcacc
    12061 caactttgca aacatgaata acataacgtt aaagtttagt aaaaaaagac tgattttggt
    12121 tagtttggta gtcaaattca gtcgattgaa attgtaatat tttggatttc aatagttttt
    12181 gtttctgaga acacatttgt agtatcccgg gattttggtg gttgtttaat ttgcattaca
    12241 gaacattgcg gaatgcattt cgatttatca gttcgctgca gtcccgatag aaaaatgctc
    12301 gagagctatt ttcgtatcaa ggaaaactac cgcgacatag cgggagtagt tcgcagttat
    12361 attctgttgg ttccgggctc cattctcgaa ttggacccca accaacgaaa gcaggttgca
    12421 attatcggca attgaacaag cagatgggaa aaaatacgct taccgaattt aaccaagcgg
    12481 ctaaaacgtt aatagaacgt ggcttggaaa aactgcctgt tcacaacgga atggtaaatc
    12541 gtggaatgga ttataaagca aaaagaattt gaacgaatat ttggcggaga tatggttcaa
    12601 caaaaccgat ttatatcttc aagcaaggat gttaatgttg ctttggattt tgccata
//
LOCUS       DAOSIB010000064        12576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_5051_length_12576_cov_8.535181, whole genome shotgun sequence.
ACCESSION   DAOSIB010000064 DAOSIB010000000
VERSION     DAOSIB010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12576
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5051_length_12576_cov_8.535181"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3721)
                     /locus_tag="PLD44_06525"
     CDS             complement(<1..3721)
                     /locus_tag="PLD44_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99024.1"
                     /translation="MKYLFTFWFSFFTTLLFAQPTRTVKFSPAIEGVANYNTITAALN
                     GATSGTVIWVAAGTYQENELVVPSGVTTVVNNVLSIENGTNTNTFTGATLTYGSSATL
                     QYNAGSSSRTVSPEWLTTFSSTGGVIVKGSGTITLNNAKTLSANVPLTINSGTLATNN
                     YNLTLGGNFTNSGTFTYGTSTVTFNGTSPQTIGGTSATNFYNLTFNNTGSSGNNTVTL
                     QQPTTVINTLTLTSGIVNTTSTNVLFVTNTAAGAIAGGSSTAFVNGPLKRSLPANYST
                     ASSYVFPVGAGTNYYPFTLVNPTTGTTAPTVQIEAKTPGPNGAVTAPLVSKSATEYWT
                     MTIGDFTNSSVSLQRPGKDNSHFSSVGGSTSASGPYVSLGGTKGTYGVTNSTSIGTNR
                     YFVLASTAFLTLSTNSLTGFTYVEGNGPSSIQSFTINAEGLTNNVVITAPEHFEVSLN
                     NGTSFSGSSQLTISAVDGAINNVTVYVRMKAGLAQGSFGPEQLTATSEGFTAQTVNLS
                     GTVTVRPVITLSPVSLTGFTYVYMSGPSAQQSFTVSGNNLQGNVILTAPTSFEISITS
                     GSGFISTLTLSPSSGTLAATTIYVRLRAGFGVTTYNENITATSLYAEMKTVSLSGSIT
                     AAATLTASPSILSGFIYSEGNGPSASQSFTLKGTNLSSNIIVTAPTNFEISTTQNGTY
                     NTSISLFPTNGTVDQILYARLKSGLAVGSYGLSNLTLSATGAVTKTVALSGKVVNTPT
                     LIASPTILGGFSYVVGGGPSSAQSFTVSGASLSTNNISITPTSLYEVSLSANSGYQAS
                     LSLTPVNGTVNATTIYVRMKTGISTAGAQNGSVSVTATSATSQTVTLNGQVYSPPSIT
                     AGGGGDYCTGSTIYLTSSGENIQSLYWTGSNSFYSTVQNPSIPNSTPAMSGAYTVTGN
                     VVVGGNLVSNGDFESGNVGFGSNYGYTTTSLVPEGLYAITTNPNSLHGNFTTHGDHTS
                     GSGYQMVVNGATNPGMVVWSQSVAVVPNADYEFSYWVQTVVNGNDSNPAQLQLYINGV
                     AMGSIFTANPTTGVWTQYVFNTNAGTNTTLNLEIVNQNTIAGGNDFALDDIVMHQIAT
                     VSSSTNVTVNDYQPVSVSVSYSPSVVYSNTLVTYTATPTNGGSSPVYKWTVNGTVVGS
                     NSPTFTYTPQNGDIVKCELRSSYPCATGNPATANVTMSVLNQYNYWMGSPTSTDWGTA
                     SNWTGGYVPLAGSNVEYATVANYGTAAVNDLQLDQD"
     gene            complement(4349..6175)
                     /locus_tag="PLD44_06530"
     CDS             complement(4349..6175)
                     /locus_tag="PLD44_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOF99025.1"
                     /translation="MNLLHLDSVSSFFQIKLLFPLFLMVGIITSPALAQNRPKQLREE
                     ILNSQKEAETKSAKSTKKKELSKPQTVLPPLTPVMPIVNPLENEKATLIYLENSETVT
                     FDKFLNPDIQVLKGNVQFRHENALMYCDSAYFYKATNSLDAFGNVRMVQGDTLFVYGN
                     VLYYDGFTKLARLRKNVRLENRKTVLTTDSMNYDRITNLAYYYTGGKIKDEVNTLTSV
                     WGQYSPATDQALFKDKVKLVNKNYTMDADTLKYNTKTNIANIVGPTHILYKNETDIYS
                     TRGWYNTSTERSMLLDRSLVIHKDGKTLTGDSIFYDKKQKYAEAFQRVILTDTVQKTT
                     LTGNFVSYNENTEFGMANDSALLVEWSTQDSLFLHADTLFTFKDSVYNAARAYYNVRF
                     FRNDLQGVCDSLYYSARDSVVRMYKEPVLWAEDFQITGDSISGYIKNKKIDHIRVLPS
                     AIAIQQFDSIHFNQISGKEIVAYLDSNQLKRVHVNGNAETIYYPLDEKDSTLIGLNKT
                     QSSYVVVYFKDKKVDRIVLTNASNGIMYPLNQLSGGDIYLKNYIWLDYLRPKSKKEVF
                     LKISRQPAARIGTSSLMNTTVNQSSSENTERNSRKSPGMRNP"
     gene            complement(6245..7606)
                     /locus_tag="PLD44_06535"
     CDS             complement(6245..7606)
                     /locus_tag="PLD44_06535"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOF99026.1"
                     /translation="MQTKRFVFFILLVSFFPFIHLTAQNNVIDEVIWIVGDDAILKSE
                     VEEQRLRAQYEGVPIQGDPYCVIPEQIAIQKLFLHQAQLDSIVANESTVNSQVEARLN
                     YFVSQIGSKEKMEEYFGKKTTELREDLRQIIREQMIIQQMQQKLVGDINPTPAEVRRF
                     FKQLPEDSIPVVPAQVELQIISYQPPVSQQEINRVKQRLLDFTERINSGNADFSVLAR
                     LYSEDVESAKRGGELGFLGRGQLVPEFAAVAFNLQDPKKVSRIVETEFGFHIIQLIEK
                     RGDRINCRHILLRPRISEEEKEKALHTMDSIANLIRSGKITFEQAVMYYSQDKNTALN
                     AGLMLNEKTGTSKFEYQDLPPEISKIAYNMNVGEISNPFIMIDPKTHKEVIAIIKVKS
                     KIPNHKANLVDDYQLIKTFYENKKKEEFLRDWIIKKQKETYISIDPAWRNCEFQYPGW
                     IKN"
     gene            complement(7675..8550)
                     /locus_tag="PLD44_06540"
     CDS             complement(7675..8550)
                     /locus_tag="PLD44_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOF99027.1"
                     /translation="MYSVFIRRFIYALILGSSVIFLACCKNRTSNTKFTDKPLVEVEG
                     NYLFRYQIEQIIPPNVNKKDSEELANNFIKNWATNVLMYERAKQNIEDISEIEKLVEE
                     YRKTLIIHAYQQKLIQQRLSTDISEEEIKSFYEKFGNQIVLNESYVKGLLLVVPISAP
                     QLNEVRKWVQQPTVKSLENIEKYRLKNAISYEYFMNKWVPFLEILKKIPLELTKTPAE
                     FLSANVFVETSDSTQIYLLKIQEYLNQGDKKPYELAKEEIKDILRHQQEVALIKQFEN
                     DLYNDALTKKKIIFY"
     gene            complement(8631..10577)
                     /locus_tag="PLD44_06545"
     CDS             complement(8631..10577)
                     /locus_tag="PLD44_06545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOF99028.1"
                     /translation="MKLSLLSLSAALSLSACLFAQTNDPVLMTINGHPVYKSEFEYIY
                     NKNNSNNSLDKKSLDEYVDLFINFKLKVEEAKAQGIDTTSAFINELAGYRSQLAKPYL
                     TDQKFEEKLMQEAYQRLKEDVEVSHILIRIPQNATPADTLAAWNKVKSILKRLEKEDF
                     AKVAKEVSEDETAEKTGGYIGWITAFQTFYPFETMAYNTPVGGISQPVRSIYGYHIIK
                     VHNRRNSVGEVQVAHIMRFTSPNDSVKNKRAKQVIDSLYQCVKNGEDFGTLASKYSED
                     KPSAARNGELPWFGTGRMVPQFEAAAFSLKKVGDVSEPIQTPFGWHIIKLLGKKDLPS
                     FNDMKSDLERRIKQDERANLAQQSFVNQLKKEYNFRLVDENVQDFYKLLKGRTLNDSV
                     FLTEIGKLNKPLFTFADKQYTQHDFANFLQKNQFTQKNIPTEVIDEKLNQFINAELMA
                     YEDTQLEKKYDDFRFLMQEYHDGILLFEVSNREVWDKALRDTVGLASYFEQHKSDYKW
                     VKPHYKGRVIYCKDKNTFKTAKLIAKRLANDSIDKYLTSRLNSDSVQYVKIEKGLFVE
                     GDNKAVDKLVFNKKEKFTPDADYPYVFVVGKLLKETPEDYTDVRGAVIADYQDYLEKE
                     WIKKLRSKYSFSVDKNVLRTVKEN"
     gene            complement(10742..12217)
                     /gene="guaB"
                     /locus_tag="PLD44_06550"
     CDS             complement(10742..12217)
                     /gene="guaB"
                     /locus_tag="PLD44_06550"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOF99029.1"
                     /translation="MSFIAERVQFEGLTFDDVLLIPSYSDVLPREVDLSTRFTRHITL
                     KIPIVSAAMDTVTESNMAIAIAREGGIGVIHKNMSIAEQARQVEIVKRAENGMISNPV
                     TILKGATVGDALALMAEFKIGGIPVVDESGYLVGIVTNRDLRFQRDFYKPVEEVMTKE
                     NLITTSRSITLDEASEILQQYKIEKLPVVDDKNKLIGLLTYKDITKAKDKPMASKDKQ
                     GRLRVAAAVGVAADTFERMDALVAAEVDAIVIDTAHGHSKGVIDMLKKARKAYPNLDV
                     IVGNIATAEAARDLAEAGADAVKVGIGPGSICTTRVVAGVGVPQLSAIFEVSKALKDL
                     DVPIIADGGIRYSGDIVKALAAGGETIMAGSLFAGVEESPGETIIFNGRKFKSYRGMG
                     SLEAMEKGSKDRYFQDMEDDIKKLVPEGISGRVPFKGSLYEVVYQLIGGLRAGMGYCG
                     AHNINELHQARFTRITNAGVVESHPHDVTIISEAPNYSRGE"
BASE COUNT         3768 a         2480 c         2291 g         4037 t
ORIGIN      
        1 tgtcctgatc gagttgcaaa tcattcactg cggctgtgcc gtaattggca acggttgcat
       61 attctacatt gctgcctgca agcggcacat aacctcctgt ccagttggaa gctgttcccc
      121 agtcggtact ggtaggactt cccatccagt agttgtattg attcagtaca ctcatggtaa
      181 cattggcagt agcgggattt ccggtagcac agggataaga agatctgagt tcgcacttaa
      241 ctatatctcc attttgtggc gtgtaggtaa aagttggact gtttgaacca actactgttc
      301 cgttgaccgt ccatttgtaa acagggctgg aaccaccatt agtgggggtg gcagtgtatg
      361 taaccaatgt attggaataa actactgatg gtgaataaga aacgctgacg ctgaccggtt
      421 gataatcgtt gacagttaca ttggtagaag aagaaaccgt tgcaatttga tgcattacaa
      481 tatcatccag cgcaaagtca ttacctcctg caatggtgtt ctgatttacg atttccaagt
      541 tcaatgtcgt attggtcccg gcattggtat tgaaaacgta ttgtgtccat acgcctgttg
      601 tcggattggc cgtaaagatg gagcccattg ctacgccgtt tatgtaaagt tgaagctgtg
      661 cagggttaga atcatttcca tttactactg tttgcaccca gtacgaaaat tcatagtctg
      721 cattgggcac tacagctacc gactgagacc aaactaccat acccggattg gttgctccat
      781 tgacgaccat ctgataacca cttcctgaag tatgatctcc atgagtcgta aaattaccat
      841 gcagtgaatt gggatttgtt gtaattgcat acaatccttc gggaacgaga gatgttgttg
      901 tatagccata attgctaccg aatccaacat tcccgctttc aaaatcacca ttggaaacaa
      961 gatttccacc tactacaaca ttccccgtta ccgtataggc gcccgacatg gcaggcgtac
     1021 tgttgggaat ggagggattt tgtaccgtag agtaaaaact gtttgacccg gtccagtata
     1081 gactttgaat attttctcca ctgctggtga gataaatggt tgaacccgta caataatcgc
     1141 ctccacctcc ggcagtgatg gatggtggac tatatacctg tccgttgagg gtaacggttt
     1201 gagaagttgc gcttgtagcg gttaccgaaa cagaaccatt ctgagctccg gcagtactga
     1261 tgcccgtctt catccgtaca taaattgttg ttgcatttac agttccattg acaggagtca
     1321 gggaaaggga agcctgatat ccggaattgg ccgacagaga tacttcgtat agtgaagttg
     1381 gagtaatact gatgttgttt gtactcagcg aagcgccact taccgtaaac gattgtgctg
     1441 aggaaggccc gccgcccaca acatagctga atcctcctaa tatggttgga gacgcgatta
     1501 aagttggcgt gtttacaact tttccactga gtgctaccgt tttggtaaca gcaccggtag
     1561 cactaagcgt taaattgcta agtccgtagg aacctacagc caatcctgat tttaatcggg
     1621 cataaagaat ttgatcgact gttccattgg tgggaaacaa agaaatggaa gtgttgtaag
     1681 tcccattttg agtggtggaa atttcaaaat tggttggtgc tgtaacgatt atgtttgagc
     1741 tgaggttggt acctttcagc gtaaatgatt gagaggccga tggcccgttt ccctcagagt
     1801 aaataaatcc gctcagaatc gacggcgagg ctgtaagggt tgctgctgcc gttatactcc
     1861 cgcttaacga aacggttttc atctcggcat acagactggt ggccgtgata ttctcattgt
     1921 aggtagtaac cccgaaacct gcccttaaac ggacatagat cgtcgttgca gcaagcgttc
     1981 cgctcgaagg ggataatgtc agggtagaaa tgaacccgga accggaagtg atggaaattt
     2041 caaaggacgt aggtgcagtg agtataacat ttccctgaag attatttccg cttaccgtaa
     2101 aactttgttg agctgaagga ccggacatat aaacataagt aaatccggtc aggctgacgg
     2161 gtgatagcgt aatgaccggc cttaccgtta cggttccact caaatttacg gtttgagcag
     2221 taaaaccttc ggaagttgcc gttaattgtt ccgggccgaa gctgccttgt gccaagcctg
     2281 ctttcatacg cacgtaaacg gttacgttat ttatggctcc atctaccgca ctgatggtaa
     2341 gctgactgct accgctaaag gaagtaccat tattaagcga tacctcaaaa tgttcaggtg
     2401 ccgttataac cacattattc gtcagtcctt ctgcattgat cgtaaagctc tgaatactgg
     2461 agggcccgtt tccttcaaca taagtaaaac ccgtaagaga gttggttgac agggttaaaa
     2521 atgccgtgga tgccaacaca aaatatcgat tggttcctat agaagtggag tttgttactc
     2581 catacgttcc ttttgttcca cccagagaaa cataggggcc actggctgaa gtgctgcctc
     2641 cgacggaact gaaatgcgaa ttgtcttttc ccggtcgttg cagagaaaca gaactgtttg
     2701 taaaatcccc gatggtcatt gtccaatatt cggttgcact tttcgatacc aaaggagcgg
     2761 taaccgctcc attaggaccg ggagtctttg cttctatctg aacggttggt gcggtagttc
     2821 cggtagtcgg atttaccaat gtaaacggat aataattggt tcctgctcct accggaaaaa
     2881 cataactact tgctgttgaa tagttggcag gcaatgatct ttttaaggga ccattgacaa
     2941 aagcagtaga acttcctccc gcgatggctc ctgctgccgt attggttaca aataaaacat
     3001 tggtcgaagt agtattcact atacctgatg tcaacgtaag cgtattgatt acggttgtgg
     3061 gttgctgaag tgtaaccgta ttgtttccgg aagatccggt attgttgaac gttaaattat
     3121 aaaagtttgt agcagatgtt cctccgatgg tttgagggga agtcccgtta aaagtaacag
     3181 tcgaagttcc ataggtaaaa gtgccactat tggtgaaatt gcctcctaaa gttaaattat
     3241 aattgttggt tgctaaagtt ccgctattta ttgttaatgg cacatttgca cttaacgttt
     3301 ttgcattatt taatgtaatt gttccgcttc ctttgacaat aacgcctccc gtactgctaa
     3361 atgtagtcag ccattcgggt gaaaccgttc gactggaaga acctgcattg tattgcaaag
     3421 tagctgaaga accataagtt aaagtagcac ctgtaaaagt gttcgtgtta gttccatttt
     3481 caatggataa tacattatta accaccgtcg taacaccaga tggaacaacc agctcatttt
     3541 cctgataggt acccgcagct acccaaatca cagtacccga cgtagcacca ttcagggcgg
     3601 ctgtgatggt attatagttt gcgacacctt caattgctgg gctgaatttt actgttcggg
     3661 tgggctgagc aaataaaaga gtagtaaaga aactaaacca aaaagtaaat aaatatttca
     3721 caattgaatt taaatgtact gattcttaaa aatataaaaa aaataaagca tagtcaattc
     3781 aaaaaaaatt aaaaattcaa aaatttttga ttcgggtaaa atattaatat tgaaattcca
     3841 aaaacgtcca gaaaattatt tagtcaaagc gtaaaattat tacaaattat tcatatctat
     3901 ttgataaaca attatttata tgttgaaatc ctattatctc ctacaatttt caaaaaccta
     3961 aatcaaagat ttcggggttg aatataattg ttttaaaagt gagttttaac gataatattt
     4021 attttttcaa taaatattat cgttaaataa aactttcgta gcaataatca acaaaaaatt
     4081 ttttacctaa ttaattatca actttttata tgattaaaac atggaatcgt catatcaagt
     4141 ccacgttttt aaatccaatt ggaaagaaaa ttaaattttt tccaaagatt ttttctgttt
     4201 ttgaagttga acccggatgc aacaggagaa ggatgtgttc attgaagata gtttcttatt
     4261 attttgatgg acaaaaaaac aaaaaactat ttctaaaata gaaataaaac gattcagaaa
     4321 gagtatgcac atggcattta aatcaaaatt atggatttcg catacccgga gattttctcg
     4381 aatttctttc cgtattttcg gatgatgact gattaactgt agtattcatc aagctgctgg
     4441 tacctattcg ggcagcaggt tgtctggaaa ttttcaaaaa aacttctttt ttgcttttag
     4501 gtctcaaata atcaagccaa atataatttt tcagatagat atcgcctccc gaaagttgat
     4561 tgagtggata catgatacca tttgatgcat tggttaagac aattctatcc acttttttat
     4621 ccttaaaata taccactaca taactgcttt gtgttttatt gagtccaatg agagttgaat
     4681 ctttctcgtc aagcggataa tatattgttt cggcatttcc atttacatgt acacgtttca
     4741 actgattgct gtccaaataa gcaacaattt cttttcccga aatctggtta aaatgaatgg
     4801 agtcgaactg ctgtatggca attgcagaag gtaaaacacg aatatgatca atttttttat
     4861 ttttaatata accgcttatc gaatcccctg ttatttgaaa atcttccgcc cacaaaacgg
     4921 gttccttgta catacggact acggaatctc gagccgagta ataaagggaa tcgcaaactc
     4981 cctgcaaatc gtttctgaaa aaacgtacgt tataatatgc gcgagctgca ttgtatacgg
     5041 aatccttgaa agtgaacagc gtgtcggcat gcaaaaacaa actatcctga gttgaccact
     5101 cgacaagcaa tgccgaatcg ttagccatac caaattcggt attctcatta taagaaacga
     5161 aatttcccgt taaggtggtt ttctgcactg tatcagtcaa tattactctt tgaaaagcct
     5221 cggcatattt ttgtttttta tcgtaaaata ttgaatcgcc cgtgagggtt tttccatctt
     5281 tatgaattac taacgatctg tccaacaaca tggaacgttc ggtactggta ttataccatc
     5341 cacgagtgga ataaatatcg gtttcgtttt tataaagaat atgcgttggc ccgacaatat
     5401 tggcaatgtt tgtttttgtg ttgtatttca acgtatcggc atccattgtg taatttttgt
     5461 ttaccagctt tactttatct ttaaacagag cctggtcagt tgcaggcgag tattgccccc
     5521 aaacagaagt caaggtattt acttcatctt ttattttccc tcctgtataa taataagcca
     5581 gatttgtaat tcggtcgtaa ttcatactgt cggtggtaag aaccgttttc cgattttcga
     5641 gcctcacatt ttttcgtaaa cgcgccaact tggtgaaacc atcataataa agaacatttc
     5701 catacacaaa caaggtgtct ccctgtacca ttcttacatt accgaaagca tccaatgaat
     5761 tggtagcctt gtaaaaatac gcactatcgc aatacattaa agcattttcg tgcctgaatt
     5821 gaacattccc cttcaaaacc tgaatatcgg ggtttaaaaa cttatcgaaa gttacggttt
     5881 cagaattttc aaggtaaatt aaggtagctt tttcgttttc cagaggattt acaataggca
     5941 ttacaggagt tagaggcgga agaacggttt gaggttttga aagttccttc tttttcgtac
     6001 tttttgcaga tttcgtctct gcttcttttt gactattcag aatttcttcg cgcaactgct
     6061 tgggcctatt ttgagctaaa gcgggggaag ttattattcc caccatcaaa aaaagtggaa
     6121 ataacaattt tatttgaaaa aaagaagaga cgctatcaag atggagtaaa ttcatgaaaa
     6181 atgattatac tattaaaaaa caaatgcgaa taacttgaaa gcaatataag catcaacaca
     6241 tgatttaatt ttttatccaa ccagggtatt ggaactcaca atttctccat gcagggtcaa
     6301 tgctgatata agtctctttc tgtttcttaa taatccaatc ccgaagaaat tcttctttct
     6361 ttttgttttc ataaaaagtt ttaatcagtt gataatcatc cactaaattt gccttatgat
     6421 taggaatttt tgatttaact tttattatag ctatcacttc cttatgggtt ttgggatcaa
     6481 tcattataaa aggatttgaa atttctccaa cattcatatt ataagctatt ttagaaattt
     6541 caggcgggag atcttggtat tcaaactttg aagttccggt tttttcattc agcatgaggc
     6601 ctgcattcaa agccgtgttt ttatcttgcg aataatacat tactgcctgt tcaaaagtaa
     6661 tttttccgct tcttattaaa ttggcaatgg aatccatagt gtgcaaggct ttttcttttt
     6721 cttcttccga aatgcgtggc ctgagcaata tatgtcggca atttatacga tcacctcgtt
     6781 tttctatcag ttgaatgata tggaaaccaa attctgtttc tacaatgcgg gatacttttt
     6841 ttggatcctg taaattaaaa gctacagctg caaattcggg taccagttgt cctctaccca
     6901 aaaaacccaa ttctcctcca cgcttggcgc tttccacatc ttcggaataa agcctggcca
     6961 atacagaaaa atcagcattt ccactattaa tacgctcggt aaaatccagt aaacgctgtt
     7021 ttactctgtt tatttcctgt tgagatacag gtggctgata actgataatt tgtaattcaa
     7081 cttgagccgg tacaacagga atgctatcct ctggaagttg tttgaaaaat cttctcactt
     7141 cagcaggagt aggatttatg tctccaacca gtttttgctg catttgctgt atgatcatct
     7201 gttcccgaat tatttgacga agatcttctc gcaattcagt ggtttttttg ccaaaatatt
     7261 cttccatttt ttccttagag ccaatttgtg aaacaaaata atttaaacga gcttccactt
     7321 gggaatttac tgtgctttca ttggctacaa tactatcaag ttgggcttga tgcaaaaata
     7381 acttttgaat ggctatttgc tcgggtatca cacaatatgg atctccttga atggggacgc
     7441 cttcatattg agcacgcaaa cgctgttcct ctacttccga tttcaaaata gcatcatctc
     7501 ctacaatcca aatgacttca tcaatcacat tgttctgagc tgtcaaatgg ataaatggga
     7561 aaaaggaaac aagtagaata aaaaaaacaa atctttttgt ttgcatgaaa ctaattaaaa
     7621 ttaaaaaaat aaaaagaaaa caagatcaga ataattgaga atactaatta ctgtttaata
     7681 aaaaattatt ttcttcttgg ttaaagcatc gttatacaaa tcgttttcaa actgtttgat
     7741 gagagctact tcttgctgat gtctcaaaat atccttaatt tcttctttag ccaattcata
     7801 cggtttttta tcaccttgat taagatattc ctgaattttt aataaataaa tttgagtaga
     7861 atctgaagtt tcaacaaaaa catttgctga caaaaattct gcgggcgttt ttgtcaattc
     7921 taaagggatt ttcttcaaaa tttccaaaaa agggacccac ttattcataa aatattcata
     7981 actgatagca ttctttaatc gatatttttc tatattctcc aatgatttta ctgtaggctg
     8041 ttgcacccat tttctcactt cgtttaattg aggagcagat atgggcacca caagtaataa
     8101 tccttttaca taagattcat tgagcactat ctggttacca aatttttcgt aaaatgattt
     8161 tatttcttcc tcagaaatgt ctgttgagag cctttgttga attaactttt gctggtatgc
     8221 atgaataatt aatgttttac gatattcctc taccaatttt tcaatttcgg aaatatcttc
     8281 aatattttgc ttggcacgtt cgtacatcaa gacatttgta gcccaatttt tgataaaatt
     8341 atttgccagc tcttcactat cttttttatt tacattggga ggaatgattt gttcaatttg
     8401 ataacgaaaa agatagtttc cttccacttc tacaagtggt ttatcggtaa atttcgtgtt
     8461 cgaagtacga tttttgcaac aagccaaaaa aatcacactg cttcccaata tcaaagcata
     8521 aataaacctc cgaataaaaa cactgtacat caaacaatta tataattcgt ttcgatttct
     8581 cgaaattgga aatataaaat tagtgatttc tatgcacctg tcaaaaaata ttagttttcc
     8641 ttaacggttc taagaacatt tttatcaacc gaaaaactat attttgaacg aagttttttt
     8701 atccattctt tttcaaggta gtcctgataa tcggcaatta cagctcctct gacatcagta
     8761 taatcttcag gcgtttcctt taacaattta ccgactacaa agacgtatgg ataatcggca
     8821 tcaggagtaa atttttcctt tttattgaat accagcttgt cgactgcctt gttgtctccc
     8881 tccacaaaaa gccccttctc aatttttaca tactgaacag agtcgctatt cagccgactg
     8941 gtcaaatatt tgtctatgga atcgttggcc aatcttttgg caatcagttt agcggttttg
     9001 aaagtgtttt tgtccttgca ataaatcaca cgtcctttgt agtgaggttt tacccactta
     9061 taatctgact tgtgctgctc aaaataggaa gccaaaccaa cagtatctct caacgcttta
     9121 tcccaaactt ctcgattgct aacttcaaac aacagaatgc cgtcgtgata ttcttgcatg
     9181 agaaatctga aatcgtcata tttcttctcc aattgagtat cttcataagc cattaattct
     9241 gcattaataa actgattcag cttttcgtca attacttctg tcggaatgtt tttttgagta
     9301 aactgattct tctgaaggaa atttgcaaaa tcgtgctggg tgtactgttt gtcggcaaag
     9361 gtaaataatg gtttatttaa tttcccgatt tcagtcaaaa atacggaatc atttaatgtt
     9421 ctccctttca ataatttata aaaatcttgt acattttcat ctactaaacg gaaattatat
     9481 tcttttttta gttgattgac aaacgactgc tgagcaagat ttgcccgttc atcctgtttg
     9541 attcgtcttt ccaaatcaga tttcatatca ttgaaagaag gtaaatcctt ttttccgaga
     9601 agtttaataa tatgccagcc gaaaggcgtt tgtattggtt cggaaacatc gcccactttt
     9661 ttcaatgaaa aagcggcggc ttcaaattgt ggaaccattc gccccgttcc aaaccatggt
     9721 aattcaccgt ttcgggctgc agaaggttta tcttcagaat atttgcttgc caaagttcca
     9781 aaatcttcgc catttttcac acactgatac aacgaatcga taacctgttt ggcacgtttg
     9841 ttttttacac tgtcattggg agaagtaaat cgcatgatgt gtgcaacctg aacttcaccg
     9901 accgaatttc ttctgttgtg aacctttata atatgatagc cataaatgga acgaacaggt
     9961 tgtgaaattc cacccacagg agtgttgtaa gccattgttt caaaaggata aaaagtctgg
    10021 aatgctgtaa tccagccaat ataaccaccg gttttctccg ctgtttcatc ttcagatact
    10081 tctttggcta ctttggcaaa atcttctttt tccaaacgtt ttaaaataga ctttactttg
    10141 ttccaagctg ccaaagtatc ggctggagtt gcattttgcg gaatccgaat gagaatatga
    10201 ctcacttcca catcctcttt cagtcgctga taagcttcct gcatcaattt ctcctcaaac
    10261 ttttgatcgg tcagataagg ttttgccaat tgtgaacgat aacctgccaa ctcatttata
    10321 aaagcagaag ttgtatcaat tccctgagct tttgcttcct caactttcaa tttgaaattg
    10381 ataaacaaat ccacatattc atccaatgat tttttatcga gagaattgtt ggaattgttt
    10441 ttgttgtaaa tgtattcaaa ttccgattta tagaccggat gcccattaat ggtcatcaat
    10501 accggatcat tggtctgtgc aaacaaacat gccgaaagcg acaatgctgc cgatagagaa
    10561 agaagggata atttcataga taaaaaacga ttaatatttt tactcactaa tttgaaacat
    10621 tgtgcttaaa aacttgcttt atcagctttt cgaaagcaaa attttcattg cactgcaaaa
    10681 aaattaacga aacaaaaaag ataatgttgt aaaaatttga ttttttttga attttaagct
    10741 tttattcgcc tctgctgtaa ttgggcgctt cgctgataat ggtaacatca tgaggatgac
    10801 tttctactac acccgcattg gtaattctgg taaatctggc ctgatgcaat tcgttaatgt
    10861 tatgcgctcc gcaataaccc atgcctgcgc gcaacccgcc aattaactga taaacaactt
    10921 cgtataaaga acctttaaat ggtacacgac cggaaattcc ttccggaaca agctttttaa
    10981 tatcgtcttc catatcctgg aaatagcggt ctttggaacc tttttccatc gcttcgagcg
    11041 aacccattcc acgataggac ttaaatttcc gtccattaaa aataatggtt tctcccggtg
    11101 attcttcaac tccggcaaaa agtgaacctg ccatgatcgt ctctcctccc gctgctaagg
    11161 ctttaacaat gtcgccggaa taacgaatgc cgccatctgc aataatgggg acatccaaat
    11221 ctttgagcgc tttagaaact tcaaaaattg cagacaactg tggaacgcca actccagcta
    11281 ctacgcgtgt tgtacaaata gaacctggac caataccaac tttaacggca tctgctccgg
    11341 cttcggccaa atcgcgagct gcttcagctg ttgctatatt tccaactata acatcaagat
    11401 tgggatatgc tttgcgtgct tttttcaaca tatcgataac cccttttgag tgcccatgag
    11461 cagtatcaat aacaatagca tccacttctg cagcaaccaa tgcatccatg cgctcaaaag
    11521 tatcggctgc aactcccact gcagcagcaa ctcttaaacg tccctgtttg tccttcgagg
    11581 ccataggttt atctttggct ttcgtaatat ctttataagt caacagacca atcagcttgt
    11641 ttttgtcatc gacaacaggt aatttttcga ttttgtattg ttgcaaaatt tccgaagctt
    11701 catccaaagt aatggaacgg gaagtcgtaa tcagattttc cttcgtcata acttcttcca
    11761 caggtttata aaaatcgcgc tgaaaacgca aatcgcgatt ggtcactatt cccaccaaat
    11821 agcccgattc atctacaaca ggaataccac caattttaaa ttcggccatc aaagccagcg
    11881 catcgccaac agtagcacct tttaaaatag ttaccggatt ggaaatcatt ccattttctg
    11941 cgcgtttaac aatttcaacc tgtcgtgcct gctcagcaat ggacatattt ttgtggatca
    12001 ctcctattcc accttctctg gctatggcga tagccatatt tgattctgtt accgtatcca
    12061 tagctgccga aactatgggg attttcaaag tgatatggcg agtaaaacgt gtggataagt
    12121 caacttcacg aggtaaaaca tcagaatatg atggaataag caaaacatcg tcaaatgtta
    12181 aaccttcaaa ttgaactctt tctgcaataa atgacataga aataaattta tttaattgat
    12241 ttttaatgag ataaataata tatattcata taacctatga aattattgca tgcaaattta
    12301 catatttatt tgttactatg gaaaaaatca tttgaaaaac taatatgata ccccatcttc
    12361 gggttctata taagccgaaa tttaaagatt tgattgaaaa aattttcggt aatgaattac
    12421 ttagtacatg acaaaaaatt gtacaaatat ttacattatt tattttcaga tatttaataa
    12481 cgcggcattg ccccaaaccc cacttacaag aaaaattgac gttcgttggc taaaatcttc
    12541 taaactcgct cctccgtcgc tcaaacagaa aagatt
//
LOCUS       DAOSIB010000065        12209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_5232_length_12209_cov_10.335445, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000065 DAOSIB010000000
VERSION     DAOSIB010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12209
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5232_length_12209_cov_10.335445"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..464
                     /gene="pyrI"
                     /locus_tag="PLD44_06555"
     CDS             9..464
                     /gene="pyrI"
                     /locus_tag="PLD44_06555"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOF99030.1"
                     /translation="MEKELKVAALRNGTVIDHIPADKLYQVVFMLHLDTCDNQITIGN
                     NLESAKIGKKGIIKIAGKVFAEDETNKIALIAPNAKINIIKDYQVIEKRPLSLPDEIH
                     EIAQCNNPNCITNHQNISTFFNVIKDETGTMLKCHYCERLIKLEEVKIK"
     gene            543..1172
                     /locus_tag="PLD44_06560"
     CDS             543..1172
                     /locus_tag="PLD44_06560"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOF99031.1"
                     /translation="MTKIDDENSSHVSWKPGTMIYPLPAVLVSCGKSADEYNLLTVSW
                     VGTICTEPPMCYISIRPERHSYEIIKRNGEFVLNLTTEKLAYATDWCGVKSGKDHRKF
                     EEMKLTPEKGKMVDAPIVKESPVCIECKVKEIVPLGTHHMFIAEVVNIQANPQYIDSK
                     TGAMHLENAHLIAYSHGHYYKLGEEIGKFGWSVRKPGKKKKVSPKIPKK"
     gene            1267..2547
                     /gene="glyA"
                     /locus_tag="PLD44_06565"
     CDS             1267..2547
                     /gene="glyA"
                     /locus_tag="PLD44_06565"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOF99032.1"
                     /translation="MKRDQEIFDIIEREKQRQMKGIELIASENFVSNQVLEAMGSVLT
                     NKYAEGYPGKRYYGGCEVVDESEQLAIDRLKKIFDAEWVNVQPHSGAQANAAVFLACL
                     NPGDKFLGLNLAHGGHLSHGSPVNSSGILYQPLQYNVKADTGRVDYDQMEEIALREKP
                     KLIIGGASAYSREWDYKRMREIADKIGAILMIDMAHPAGLIAAGLLENPLKWAHIVTS
                     TTHKTLRGPRGGIILMGKDFPNPWGKTTPKGEVKMMSALLDSAVFPGIQGGPLEHVIA
                     AKAVAFSECLQPEFFEYQKQVKKNAEKLANELISRGFNIVSGGTDNHCMLVDLRSKFP
                     DLTGKMAENALVKADITVNKNMVPFDSRSPFQTSGIRLGTPAITTRGAKEDLMTEIAE
                     MIETVLQNADNEKIILQVKEKVNKMMKDFPLFAY"
     gene            2680..3675
                     /locus_tag="PLD44_06570"
     CDS             2680..3675
                     /locus_tag="PLD44_06570"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOF99033.1"
                     /translation="MNVKGKIAIIGGGSWATALAKIALSNEQQINWYMRRQEQIDEFI
                     RQSHNPSYLTGVEFDTDRINFTSDINKVVKSSDVLIFATPSPFFKGHLKKLRQKLDKK
                     FVVSAIKGIVPEENMVISEYFEQFYNVSPENMAVLAGPCHAEEVAMERLSYLTVACAS
                     REKARMLADRLATNFIKTSVSDDVLGIEYASVMKNIYAIAAGICHGLKYGDNFQAVLV
                     SNAIREMNRFCNAMNALHRDISESAYLGDLLVTSYSKFSRNRLFGTMIGKGYSVKTAQ
                     MEMEMIAEGYYATKCIHEINEKVQISMPIVDTVFQILYNRCSPIIEIRKLTEQLM"
     gene            3699..5042
                     /locus_tag="PLD44_06575"
     CDS             3699..5042
                     /locus_tag="PLD44_06575"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOF99034.1"
                     /translation="MEHIQFFSDKSSVSKEKIAEYKEKAIRCISALHEGTGKGSDFLG
                     WLHLPSSIDEAQFAEIENTAKLFQKSCEVVVVIGIGGSYLGAKAVIDALSNSLDWLQK
                     VRKSPIIVYAGHNISEDYLFELQEFLKDKKFGIISISKSGTTTEPALAFRLLKTQLEE
                     QQGKAEAKKLIVAITDRSKGALRKLAEQEGYKTFVIEDNIGGRYSVLTPVGLLPIATA
                     GFDIRQLVAGAAFMESLCGMDTPFEKNPAAQYAVVRNELYQQKGKKIEILVNFHPKLH
                     YVSEWWKQLYGESEGKDHKGIFPAAVDFTTDLHSMGQWIQEGERSIFETVISIKEPNH
                     LKTVPFDPENLDGLNYLSGKRVDEVNKMAELGTMIAHVDGGVPNLKVEMPKLNEYYLG
                     ELLYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLEKPGYEQETKAIKEKLK"
     gene            complement(5164..6819)
                     /locus_tag="PLD44_06580"
     CDS             complement(5164..6819)
                     /locus_tag="PLD44_06580"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOF99035.1"
                     /translation="METNKSPLQIARSSYQPKIPTILSGNIALKYGEPTESVADQKEI
                     KNLFPKTYGLPIITFEEGAQVEYPEIAVGVILSGGQAPGGHNVIAGLFDGLKKLNPKN
                     KLYGFLDGPSGLVDHKYTELTSEIIDEYRNTGGFDIIGSGRTKLELEWQFEQGAEICR
                     KLKIKAIVIIGGDDSNTNACVLAEYYARKNFGIQVIGCPKTIDGDLKNEMIETSFGFD
                     TACKVYSELIGNIQRDASSAKKYWHFIRLMGRSASHITLECALQSQPNITLISEEVEG
                     KNLSLSDIVEYIVQIIVQRANHGINYGTVLIPEGLIEFIPGMKKLIQELNDLLANNED
                     FVALHTDDEKRQYIKGMLSPEASQLYRSLPKGIARQLSMDRDPHGNVQVSLIETEKLL
                     IEMVAKRLAQLKAEGVYKGIFSAISHFFGYEGRCAIPSNFDANYTYTLGIVASLLIAE
                     GKTGYMASVKNLTDEPANWIACGVPLTMLMNMEKRNQKMKPVIQKALVKLDGAPFKYL
                     EAHRDEWADEKVSYIYPGPIQYFGPTEVCDLPTKTLLIEHGKL"
     gene            7182..7856
                     /locus_tag="PLD44_06585"
     CDS             7182..7856
                     /locus_tag="PLD44_06585"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOF99036.1"
                     /translation="MCKNSFRNNVLIVFMILPLALQAQDQYLAEIGVTAGNGYYLGDA
                     NEMPFKNSSLVYGSVFRYRIDPRLSVRVEWNHGTVEGKYFNSTGETLNFGNSFNAVDI
                     NGEFNFFDLMQKSNSRNSKKYSTYIFAGLGTTIYANESSNTVTAFSIPFGVGFKYKVS
                     TRFNVNLQWNMRWLSSDDLEAVNDLNNAQRLNGSNPLNKDFFSTVTLGITFDFLKKPC
                     DCKSKW"
     gene            7872..8591
                     /locus_tag="PLD44_06590"
     CDS             7872..8591
                     /locus_tag="PLD44_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOF99037.1"
                     /translation="MFKEKIDYTHLPKHIAIIMDGNGRWAKEKGYNRIYGHQKGVTSV
                     RRITEAAAESNIQFLTLYTFSTENWNRPSDEIKALMELLVITIDKEMEDLNKNNIRML
                     TIGDLSKLPNDAAEKIRECKAKTAQNTGLTLNLALSYSSRWEITEAVKNIVCDVEKGK
                     LTSKNISDKLISSYLSTAGIPDPDLMIRTGGELRISNFLLWQSAYTEFYFTSKYWPEF
                     DKEDFYEAIFDFQQRERRFGR"
     gene            8629..11286
                     /locus_tag="PLD44_06595"
     CDS             8629..11286
                     /locus_tag="PLD44_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOF99038.1"
                     /translation="MKNKIYFILFLIFFHQLSSFSQNSKNDSTSIATENESVPLPVIE
                     YSNTNPKYEIAEITVTGADHYEDFVLIGFSGLSVGQVISVPGPEITEAVKRFWKQGLF
                     SDVKIYATKIENGKVWLNISLKERPRISKINYSGLKKGEIEDLEKKLNLSVGNQITPN
                     ITDRIITVIKKYMEEKGFLNTDVFVYQKDDPEKPGHVIVDIEVNKKIKTSVHKIYVTG
                     NQALTVAQIDKAMKKTNEKNLRNFFRSKKFVKQEYENDKKLIIEKYNEIGYRDAYIVT
                     DSIVPFNEKSVDIYLTINEGKKYYFRNISWIGNTVYPYEFLNEKLGIKKGDLYNYNYL
                     IKRLLTDENDAISKLYQNNGYLFFQIEPVEVHVENDSIDFEIRIYEGKQATINEIGIK
                     GNTRVYEHVIRRELRTKPGQLYSQEDIVRSLRELAQMGHFDQEKMNNAIDIQPDPENG
                     TVDINYVLESKGNDQIEFSAGWGATGVVGSVGLKFSNFAIQNLFNPDTYRIVPQGEGQ
                     TFSLNARTNGQSYSSFSISFLEPWLGGKRPNSLSASVYYSTQTAISNRYLNYINNLSS
                     MYYGGYYGGYYNDYYGNNYNYYQTEVDPDRYMRTLGASLGYGKRLNWPDDYFSFYSEL
                     SFQRFMLNNWYAGYFPMTSGTFNNLSMNLILNRSSIDNPIYTRTGSLFSLGLKFTPPY
                     SVFNGKDYSQEMSNEERYKFIEYHKWTFTGKTFTPLTPNNKLVLMARAQFSYLGYYNK
                     NARSPFETFYMGGDGMSSYTSYGTEYVAMRGYENGSLTPYDTKTGIAAGYLYNKYTME
                     LRYPLSLEQSATIYVLSFVEAGNCFNSISGPNGYNPFNLKRSAGIGVRIFLPMFGMMG
                     IDWGYGFDTVTGATKPSGSQFHFVLGQEL"
     gene            11352..11867
                     /locus_tag="PLD44_06600"
     CDS             11352..11867
                     /locus_tag="PLD44_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOF99039.1"
                     /translation="MKKLIVTFCFLSVFTWLSSAQKFASVDMSYILKNIPAYETANEQ
                     INQVSKKWQSEVEALQKEAEAMYKNYQTELVFMTEEMKKKREEEIVAKEKEAQELKRK
                     YFGPQGELFKKRESLMKPIQDEVYTAIQSISKDRNIEVVFDKSTAMSVVFTTPGIDIS
                     DEVLKQLGYSK"
     gene            11926..>12209
                     /locus_tag="PLD44_06605"
     CDS             11926..>12209
                     /locus_tag="PLD44_06605"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOF99040.1"
                     /translation="MFKKIAFLLLCVFPISLMGQEVKLGHINSQEILSLMPERTEIEK
                     TLNDLQTQWENELLKMREEYYAKIKEFQDKQATMPESIKQARQSEIVEIE"
BASE COUNT         4113 a         2152 c         2344 g         3600 t
ORIGIN      
        1 aaagcatcat ggaaaaagag ctcaaagtgg cagcattgcg taatggaacg gttattgacc
       61 atattcctgc cgataaattg tatcaggtgg tattcatgtt gcacttggat acctgcgaca
      121 atcagatcac aattggcaac aatctggaga gtgccaaaat aggaaaaaaa ggcatcatta
      181 aaatagccgg aaaagtgttt gctgaggacg aaaccaataa aattgccctc attgctccca
      241 atgctaaaat aaacattatc aaagactacc aggtgattga aaaacgacca ctttcgttgc
      301 ctgacgaaat tcatgaaatt gctcagtgca acaatcccaa ttgcataacc aatcatcaaa
      361 atatttccac atttttcaac gtaatcaagg atgaaaccgg tactatgctt aaatgtcatt
      421 attgtgaaag actgataaaa ctggaagaag ttaaaattaa atgaaatttt tttttggata
      481 aataggcatt ttgaaagtaa tttgacagcc atccaattta ttattattca aacatttctg
      541 caatgacaaa aattgatgat gaaaattctt ctcatgtttc atggaaaccg ggtacaatga
      601 tatatccgct ccctgctgtt ctcgtttcgt gcgggaaatc ggccgatgaa tataatttgc
      661 tgacagttag ttgggtggga acaatttgta cagagcctcc catgtgttat atttccatac
      721 gtcccgaacg gcattcttat gaaattataa aaagaaatgg cgagtttgta ctcaatctta
      781 ctacggaaaa attggcttat gctaccgact ggtgcggtgt aaaaagtgga aaagatcatc
      841 gcaagtttga agagatgaaa cttacacccg aaaagggaaa aatggttgat gcacctattg
      901 tcaaagaatc tcccgtatgt atcgaatgta aggttaaaga aattgttccg cttggaacgc
      961 atcacatgtt tattgccgaa gtagttaaca ttcaggccaa tccacagtat attgacagca
     1021 aaacgggagc catgcacttg gaaaatgcac atttgatagc ttattcgcac ggacattatt
     1081 acaaacttgg agaagaaatc ggaaaatttg gttggtcggt aaggaaaccc ggcaaaaaaa
     1141 agaaagtttc cccaaaaatt ccaaaaaaat agaaaaaact ttatcaacat ctcagaattt
     1201 gtttcgttaa aagaacgaaa aacaacaagt gtaattcata ttttttaaaa taataaagaa
     1261 cagaaaatga aaagagacca ggaaattttt gacattatcg aacgcgaaaa acagcgacag
     1321 atgaaaggca ttgagttgat agcatcggaa aattttgtga gcaatcaggt gctggaagca
     1381 atgggatcgg ttttgaccaa caaatatgct gagggttatc cgggaaaacg atattatggc
     1441 ggttgcgaag tggtggacga atcggaacag ctggctatcg accgattaaa aaaaatcttt
     1501 gatgccgaat gggtcaatgt acagccacat tcaggtgcgc aagccaatgc tgccgtattt
     1561 ttagcctgcc ttaatccggg agataaattt ttaggtttaa atcttgctca cggaggccat
     1621 ttatcgcatg gttcaccggt caacagttcg ggaattttgt atcaaccctt gcaatacaac
     1681 gtaaaagccg atacgggaag agtcgattat gaccaaatgg aagaaattgc gcttcgcgaa
     1741 aaaccaaaat tgattattgg tggagcttca gcctattcgc gagaatggga ttataaaaga
     1801 atgcgcgaga ttgccgacaa aattggtgct attttgatga ttgacatggc tcatcctgca
     1861 gggctgatag ctgccggatt acttgaaaat cctttaaaat gggctcacat tgtaacttct
     1921 accactcata agaccttgcg tggacctcgt ggaggaatta ttttgatggg aaaagatttt
     1981 ccaaatcctt ggggaaaaac gacccccaaa ggagaagtaa aaatgatgtc ggctttgctc
     2041 gattctgcag tttttcccgg tattcagggt ggcccgctcg aacatgttat tgcggccaaa
     2101 gctgtggctt tcagtgaatg tttgcaaccg gaatttttcg aatatcagaa acaggttaaa
     2161 aagaatgcgg aaaagctggc caatgaactt atttccagag gtttcaatat tgtttcaggt
     2221 ggtacggaca atcattgtat gctggttgat ttaaggagca aattccccga tttgacaggc
     2281 aaaatggctg aaaatgcttt ggtaaaagcc gatataacgg tcaacaaaaa tatggttcct
     2341 ttcgatagtc gatctccatt ccaaacttcc ggtattcgac ttggtactcc tgccataaca
     2401 acgcgtggag ccaaagaaga tttgatgact gaaattgcag aaatgattga aaccgtttta
     2461 caaaatgcag ataacgaaaa aattatcctt caggtaaagg agaaagttaa taagatgatg
     2521 aaggattttc ctttgtttgc ctactaaaag tgcataaaaa taaggaattt tttcaaaata
     2581 ctgtttgaat tttaaaaatg ttttgatttt gtaaaaagaa aaatcaatat ctttgcagga
     2641 ttttgaatat taattttgta attattttgc agcgtgggaa ttaacgttaa agggaaaata
     2701 gccattattg gcggaggaag ttgggctaca gctctggcaa aaattgcttt gagcaacgaa
     2761 cagcaaatca attggtatat gcgccgacag gagcagatag atgaatttat tcgccaaagc
     2821 cacaatccat cctatcttac cggtgttgaa ttcgatacag accgaattaa ttttaccagc
     2881 gacataaaca aagtggtaaa atcgtccgat gtgttgattt ttgctactcc atctcctttt
     2941 tttaagggac atttgaaaaa attgcgtcaa aagctggaca aaaaattcgt tgtttcagcc
     3001 atcaagggaa ttgtgccgga agaaaacatg gttatttcgg aatactttga gcaattttac
     3061 aatgtttcgc ccgaaaatat ggccgttttg gccggtccgt gtcatgctga agaagtggca
     3121 atggaaagac tttcctatct gaccgttgct tgcgcttcaa gagaaaaagc ccgtatgctg
     3181 gccgataggt tggctaccaa ttttattaaa acttcggtta gcgacgatgt gttgggaatt
     3241 gagtatgctt ccgtcatgaa aaatatttat gccattgctg cgggtatttg tcacggatta
     3301 aaatatggcg acaattttca ggccgttctc gtctcgaatg ccattcgtga aatgaataga
     3361 ttctgcaatg ccatgaacgc tcttcatcgc gatatttccg agtcggctta tctaggtgat
     3421 ttgttggtta cgtcttattc aaaattcagc agaaaccgtc tgtttggtac catgattgga
     3481 aaaggttatt ccgtaaaaac ggcacaaatg gaaatggaaa tgattgctga aggttattat
     3541 gccaccaaat gtattcacga aattaacgaa aaagttcaaa tcagcatgcc tattgtcgat
     3601 acggtttttc aaatcctgta taatcgttgt tctcccatta tcgaaattcg taaactgaca
     3661 gaacaattaa tgtaaataac aaaaaaattt ttaataaaat ggaacatata caatttttta
     3721 gcgacaagtc ttcagttagc aaagaaaaaa tcgctgaata taaagagaaa gccattcggt
     3781 gcatttcggc acttcatgaa ggaacgggaa aaggaagcga ttttttgggt tggctgcatc
     3841 ttccgagcag tattgatgaa gctcaattcg ctgaaataga aaataccgcc aaacttttcc
     3901 agaaaagttg tgaagtagtc gtagtaatcg gaataggtgg aagttatctc ggcgcaaaag
     3961 cggtgattga tgccttgtcg aatagtttag attggttgca gaaagttcgc aaatcaccca
     4021 ttattgttta tgcgggtcat aatatcagtg aagattattt gtttgaactt caggaatttc
     4081 tcaaagacaa aaaatttggc atcatcagta tttccaaatc gggaactact acagaacctg
     4141 cattggcttt ccgacttctc aaaactcaat tggaagaaca gcagggaaaa gccgaagcaa
     4201 aaaaactgat tgttgccatt acggaccgtt ctaaaggtgc gttgcgtaaa ttagccgaac
     4261 aggaaggtta taaaaccttt gtcatagaag acaacattgg cggacgttat tctgtgctga
     4321 cacctgtagg cctgcttccc atcgcaactg ccggcttcga cattcgtcag cttgttgcag
     4381 gagcagcctt tatggaatcg ctttgtggaa tggatacgcc atttgaaaag aatcccgctg
     4441 cacaatatgc tgtggtgaga aatgagcttt atcagcaaaa aggcaaaaaa attgaaattc
     4501 ttgtcaattt tcatcctaaa ttgcactatg tttccgaatg gtggaaacaa ctttatggtg
     4561 aaagtgaagg aaaagatcac aagggaattt ttccggctgc agttgatttt acaaccgacc
     4621 tgcattctat gggacaatgg atacaggaag gcgaaagaag tatttttgaa acggttattt
     4681 ctattaaaga gcccaatcat ttaaaaactg ttccttttga tcctgaaaat ttagatggtt
     4741 tgaattattt gtcggggaaa cgggtagacg aagtcaacaa aatggcagaa cttggcacca
     4801 tgatcgctca tgttgatgga ggagttccaa acttgaaagt ggaaatgccg aagttgaacg
     4861 agtattattt gggtgaattg ctttatttct ttgaaaaggc ttgcggtatc agcggttatt
     4921 tgcttggtgt aaatcctttt gatcaacccg gagtggaagc ttataagaaa aatatgtttg
     4981 ctttgctcga aaagcctgga tatgaacagg aaacgaaagc cataaaagaa aaattgaaat
     5041 aagtattgga tttcaatcat ttatacagtt aaggctgctt caacgaggca gccttaactg
     5101 tataaattaa aatgatttgt ctattacgaa aattttatac aaaaatatat tcatccttga
     5161 atgttataat tttccgtgtt cgattaataa agttttggtt ggtaaatcac acacttctgt
     5221 tggaccaaaa tattgaatag gtccgggata aatgtagctt accttttcgt cagcccattc
     5281 atctcgatgg gcttccaaat atttaaaagg tgcaccatca agctttacta atgccttttg
     5341 tatcacgggt ttcatttttt gattcctttt ctccatattc atcaacatag ttaaaggcac
     5401 accacaagca atccaatttg caggttcatc tgtcaaattt ttcactgaag ccatataacc
     5461 ggtttttcct tcagcaatta aaagtgaggc aacaattcct aatgtatagg tgtagttggc
     5521 gtcaaaattg gatgggatgg cacaacgtcc ttcataacca aagaaatgac tgatagcaga
     5581 aaagattccc ttataaacac cttccgcttt caattgagcc aaacgttttg ccaccatttc
     5641 tatcaataat ttttcagttt caatgagcga cacttgaaca tttccatgag gatcgcgatc
     5701 catagaaagc tggcgtgcaa ttccttttgg taaacttctg taaagttgac ttgcttcagg
     5761 gctcaacata cccttgatat attgacgttt ttcatcatcg gtatgaagag ctacaaaatc
     5821 ttcattatta gccaacaaat cgtttaattc ctgaataagt tttttcattc cgggaataaa
     5881 ctcaatcaat ccttcaggga taagtactgt tccatagttg ataccatgat tggctcgttg
     5941 aacgataatt tgaacaatat attccacaat atcgcttaaa ctcagatttt tcccttcaac
     6001 ttcttcggaa ataagtgtta tattcggctg actttgaagg gcacattcca gtgtgatatg
     6061 cgaagccgaa cgacccatca gacgaataaa atgccaatat ttttttgcag agctggcatc
     6121 tcgttgaata tttccaataa gttccgaata aactttacat gctgtgtcaa aaccaaaaga
     6181 tgtttcaatc atctcgtttt tcaaatcacc atctatcgtt ttagggcaac ctattacctg
     6241 aataccaaaa tttttccgtg cgtaatactc agccaacacg caagcattgg tgttagaatc
     6301 atctcctccg atgatgacta tcgcttttat ttttaacttt ctgcaaatct cggctccttg
     6361 ttcaaattgc cactccagtt ctaattttgt tcttcctgaa cctataatat caaatcctcc
     6421 agtattacga tattcatcga taatttccga agtcagttca gtatatttat gatcaaccag
     6481 accacttgga ccatccaaaa aaccataaag tttattttta ggatttaatt tttttaatcc
     6541 gtcaaagagt cctgcaatta cattatgtcc acccggagcc tgcccgcccg acaaaataac
     6601 acccacagca atttcgggat attcaacctg tgccccttct tcaaaagtaa taatcggtaa
     6661 tccgtaagtt ttaggaaata aattctttat ttctttctgg tcagcaaccg attcggtagg
     6721 ttcgccatat tttaatgcaa tgtttccact cagaatagta ggtattttgg gttgataact
     6781 tgaacgtgca atctgcaagg gactcttatt ggtttccata aaaaattttt taaagggttg
     6841 atatgtaaat gtaagtcttt ttgaaaatct atttgcaaaa ttaatcataa aatagaaaaa
     6901 caacaagcaa aaagcattca attttcaata agcacaaata taatttcaac ttttaaatat
     6961 cattttcgat ggtgaaaaga ttaaattttg agtaaaaaca caaactattc atttgcaatt
     7021 ttttgttaaa taaattattg agttttcaat caactctgct aaattgaaag attatcacta
     7081 tttttgtagc tctattctgc ctgaggagaa aatttatatt caaaaaacac gttatatcat
     7141 tactttttaa tagtgtctta tcatattagt tacatcaaat tatgtgcaag aattccttta
     7201 ggaataacgt gctgattgtt tttatgattc tacctcttgc attacaagcg caagatcaat
     7261 atctggcaga aattggagta acagccggca acggatatta tttgggcgat gcgaacgaaa
     7321 tgccttttaa gaattccagt ttggtttatg gttctgtttt tcgctataga attgatccgc
     7381 gtttatccgt acgggtagaa tggaatcatg gtactgtaga agggaaatat tttaattcta
     7441 cgggcgaaac attaaatttc ggcaattcat tcaatgccgt agatataaac ggagaattta
     7501 atttctttga tttgatgcaa aaatcaaata gccgtaacag caaaaaatat tcaacatata
     7561 tttttgccgg tttgggaaca acgatttatg ctaacgaaag tagcaatacg gtaacagctt
     7621 tcagtattcc gtttggagtc ggatttaaat ataaagtcag cacacgtttc aatgtaaatt
     7681 tacagtggaa tatgcgttgg ttaagtagtg atgatttgga agcagttaat gatttgaaca
     7741 atgcacaaag attaaatggt tccaatccat tgaataaaga ttttttttcg actgttactt
     7801 taggaatcac ttttgatttc ttaaaaaaac cttgcgattg caaatcgaaa tggtaaaaat
     7861 gaaaaccatc tatgtttaaa gaaaaaattg attatacgca tctgcccaaa catatagcta
     7921 taataatgga tggcaacgga cgttgggcaa aagaaaaagg atacaacaga atttacggac
     7981 atcagaaagg cgtaacttca gttcgtagaa ttacagaagc agctgcagaa tctaacattc
     8041 agtttcttac gctttatact ttttccaccg agaactggaa tcgtcccagc gatgaaataa
     8101 aagcattgat ggaattgctg gtaattacta ttgataaaga aatggaagat ttgaacaaaa
     8161 acaacattcg aatgctcacc attggcgatt tgtcgaaatt accaaacgat gccgcagaaa
     8221 aaatccgtga atgtaaagct aaaacagctc aaaatacggg actcaccttg aatctggcat
     8281 taagttattc ttcccgatgg gaaattaccg aagccgtaaa aaatattgtt tgcgatgtag
     8341 aaaaaggaaa actaacttca aaaaatattt ccgacaaact aatttcctct tatttgtcca
     8401 ctgccggcat tcccgatcca gacttaatga taagaacagg aggcgaacta agaatcagca
     8461 acttcttgtt atggcaatcg gcatataccg aattctattt tacttccaaa tattggcctg
     8521 aattcgataa agaagatttt tacgaagcta tatttgattt tcagcaacgg gaacgtcgtt
     8581 ttggtagata aataaatgat atataaaaaa ttgactgatt gaatttttat gaaaaataaa
     8641 atttatttta ttctattctt gatatttttc catcaactct cttctttttc gcaaaattcg
     8701 aaaaacgatt cgacctctat tgcaactgaa aatgaatcag taccattgcc ggttattgag
     8761 tattcaaata caaatcccaa atatgaaatt gcagaaatta cggtaactgg ggccgaccac
     8821 tacgaagatt tcgtattgat tgggttttcc ggtttatctg tagggcaagt gataagcgtt
     8881 ccgggaccgg aaattaccga agccgtaaaa cgattttgga aacagggtct tttttctgat
     8941 gttaaaatct atgccaccaa aatagaaaat ggaaaagtat ggttgaatat ttcattgaaa
     9001 gagcgtccca gaatatcaaa aatcaattat tccggtttaa aaaaaggtga aatagaagat
     9061 ttggaaaaaa aattaaattt atctgtcggc aatcaaatta caccaaatat tacggaccga
     9121 ataattacgg ttataaaaaa atacatggaa gaaaaaggtt tcttaaacac tgacgttttt
     9181 gtttaccaaa aagacgatcc cgaaaaaccg ggacacgtga ttgtggatat cgaagtgaac
     9241 aagaaaatta aaaccagtgt ccataagata tatgtaacag gaaatcaagc tttgacagtt
     9301 gcacaaattg acaaggccat gaaaaaaacc aacgaaaaaa atttacgcaa cttttttcgc
     9361 agcaaaaaat tcgtaaaaca agaatacgaa aacgacaaaa aactaattat agaaaaatat
     9421 aacgaaatcg gttatcggga cgcctatatt gttacggata gtattgttcc ctttaacgaa
     9481 aaaagcgttg acatttatct caccatcaat gaaggtaaaa aatattattt cagaaatatc
     9541 agttggatag gtaacacggt ttatccttac gagtttttaa acgagaaact aggaataaaa
     9601 aagggagacc tttacaatta caattattta atcaagcgcc ttctaacaga cgaaaacgat
     9661 gccataagca aattatatca gaataacggc tatctgtttt ttcaaattga accggtagaa
     9721 gtacatgtag aaaacgattc tattgatttt gaaattagaa tttatgaagg taaacaagct
     9781 accatcaacg aaattggtat aaaaggaaac acccgcgttt acgaacatgt gataagaaga
     9841 gaattgcgaa ctaaacccgg acaattgtac agtcaggagg atatcgtccg ttccttgcgt
     9901 gaactggcgc aaatgggtca tttcgaccaa gaaaaaatga ataatgccat tgatattcaa
     9961 cccgatccgg aaaatggcac cgttgatatt aattatgtgc tcgaaagcaa gggaaacgat
    10021 caaatagaat tttctgccgg ttggggagca acaggagttg ttggtagtgt aggattaaaa
    10081 ttcagtaatt ttgccatcca aaatttattt aatcccgata cttatcgtat tgtaccacag
    10141 ggagaaggtc aaactttttc tttaaatgcc cgcaccaacg gacaatctta cagttctttc
    10201 agtatttcgt ttcttgaacc atggttggga ggaaaacgcc ccaattcact ttctgcctca
    10261 gtatattatt ctacacaaac tgccattagc aaccgatacc tgaattacat caataatctt
    10321 tcgagtatgt attatggtgg ttattacgga ggatattaca acgattacta cggcaataac
    10381 tataattact atcaaacgga agtagacccc gaccgctata tgcggacact gggggcatcc
    10441 ttaggctatg gaaaaagatt gaattggcct gacgattact tctctttcta ttccgaactt
    10501 tcatttcaac gtttcatgct gaacaactgg tatgccggat attttcctat gacttcgggt
    10561 accttcaaca atctaagtat gaatctgata ttgaaccgtt cttccataga taatcccatt
    10621 tatacccgta ccgggtcatt gttctcatta ggacttaaat ttacgccccc ttattctgtt
    10681 ttcaatggca aagactattc tcaagaaatg agtaacgaag aacgatataa atttatagaa
    10741 tatcacaaat ggacatttac cggaaaaact tttacgccac ttactcccaa caataaattg
    10801 gtacttatgg caagagcaca gttttcctat ttaggttatt acaacaaaaa tgcccgatca
    10861 ccttttgaaa ctttctacat gggcggcgac ggaatgtcgt cctacaccag ctatggcacc
    10921 gaatatgtgg ccatgcgtgg ttacgaaaac ggttcactca caccttacga taccaaaaca
    10981 gggattgcag caggttattt gtacaacaaa tatactatgg aactgcgtta tccgctttcg
    11041 ttggaacagt ctgctacaat ttatgtttta agttttgttg aagcaggaaa ctgtttcaac
    11101 agtatttccg gtcccaatgg ttacaaccca tttaatctga aacgatcggc aggtataggt
    11161 gtacgcatat ttcttcctat gttcggcatg atgggtatag actggggtta tggattcgat
    11221 acggttaccg gagcaactaa accaagcggc agtcagttcc atttcgttct tggacaggaa
    11281 ttataataca ttagattttc tggcaaagaa tttgttataa attaaaatta ttaagattaa
    11341 atctttcgat tatgaaaaaa ttgatagtaa cattctgttt tttaagtgta ttcacatggt
    11401 tatcttctgc acagaaattt gcctcagtgg atatgagcta tattttgaaa aatattccgg
    11461 catacgaaac tgccaacgaa caaatcaatc aggtttcaaa aaaatggcag tcggaagtag
    11521 aagctcttca gaaagaagct gaagccatgt ataaaaatta ccaaaccgag ttggtattta
    11581 tgacagaaga gatgaagaaa aagcgtgaag aggaaattgt tgcaaaagaa aaagaagcac
    11641 aggaactgaa aagaaaatac ttcggcccgc aaggagaact tttcaaaaaa agagaaagtt
    11701 tgatgaagcc tattcaagat gaagtttaca cagccattca aagcatcagc aaagatcgaa
    11761 atatagaagt ggtttttgat aaaagcacgg ccatgagtgt agtttttaca acacccggca
    11821 tcgacattag cgatgaggta ctcaaacagt tgggttattc aaagtaattt aatatatttg
    11881 cactctgttt ttcaaatttg tataatcaac atcaaaaaaa ttgtcatgtt taagaaaatt
    11941 gcctttttat tattatgtgt cttccctatc agtttaatgg gtcaagaagt aaaattagga
    12001 catatcaatt cgcaggaaat tctttcttta atgccggaaa gaaccgaaat cgaaaaaact
    12061 ttaaacgatt tgcaaacgca atgggaaaat gaactgttaa aaatgaggga agaatattac
    12121 gctaaaatca aagaatttca ggacaaacaa gccaccatgc ccgaaagcat caaacaggct
    12181 cgccaaagtg aaattgttga aatcgaaca
//
LOCUS       DAOSIB010000066        11644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_5508_length_11644_cov_11.892743, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000066 DAOSIB010000000
VERSION     DAOSIB010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11644
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5508_length_11644_cov_11.892743"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..528)
                     /locus_tag="PLD44_06610"
     CDS             complement(64..528)
                     /locus_tag="PLD44_06610"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOF99041.1"
                     /translation="MKKQLKIALVAHDNRKADMVEWAVYNADMLSQHKLICTGTTGKL
                     IQRAMEEKGVKVDITCMHSGPLGGDAEIAALVVQKQINLAVFLIDDLNPQPHEADIQM
                     LLRQCRIHNVPIACNRYSADLMITSTLWDDENYIPTEPKYVKFVREDLFNFE"
     gene            996..3317
                     /locus_tag="PLD44_06615"
     CDS             996..3317
                     /locus_tag="PLD44_06615"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOF99042.1"
                     /translation="MNLDFLNDLNESQRQAVEYMDGASLVIAGAGSGKTRVLTYKIAY
                     LLTKGFPPSSILALTFTNKAAREMKERIAQIVGERTARYLWMGTFHSIFSRILRNEAQ
                     YTGYSKNFTIYDSSDSSSLIKSILKERNLDTKTYPPGLIQSRISSAKNNLITPEAYAV
                     NSDFIKSDKQSRIPLLSEIYQTYCNRCKQADAMDFDDLLLQTNLLFRNHPEVLEKYQQ
                     LFQFILVDEYQDTNFAQYLIIKKLAQQHERISVVGDDAQSIYSFRGANIDNILQFKNA
                     YHNARIFKLEQNYRSTQTIVNAANSLIDKNRAQIQKTIFSEKEIGHPIKLLNVFTDYE
                     EGLTVAKHILDLYALYDYRYADIAVLYRTNSQSRILEETFRKQGIPYRIYGGLSFYQR
                     KEIKDVIAYCRLVCNQNDEEALKRIINFPTRGIGETTVGKLVECAQSKDVGIWEVLSN
                     MTKYGVPVNAGTASKLNDFRNLIESFSQQLEIKNAYDLAVMIVKTTGLLNEANFDHSP
                     ESISRKENIEEFLKAIHEFCEKKMEEGETLPLLPDFLSEVSLLTDQDTDKDSLSDKVT
                     LMTVHAAKGLEFKAVFIVGMEEELFPSMFSLVNERELEEERRLFYVAITRAKERCFIS
                     YAKSRFRNGKTNFCTPSRFIKDIDPQFLEITEEKAPTFQKKNFMNWDEEIESERNRFY
                     SPTNEKETYSEPQTTNFSPRKLKKLDQSSDEKIIIENSSFPVGTIVKHQLFGIGKVLE
                     TSISNGNEKATIDFGEKGVKSLLLKFAKLEVVK"
     gene            complement(3846..4463)
                     /locus_tag="PLD44_06620"
     CDS             complement(3846..4463)
                     /locus_tag="PLD44_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOF99043.1"
                     /translation="MEKVNRIIFIALLTVAFFGCKSTPTKTIANLKEGIKGETTASAK
                     YSAFAQKAREEGYDTIAKLFDAASKAEAIHAANHTKVLESLGEKMEAFTPSFEVKSTA
                     ENLQAAIDGESYESTTMYVNFINDANAEKVTDAVKSFTWALDTEKKHLDFYQKALAAL
                     KANNESTLPSAYAVCPVCGNTYDNAAIDPNCAFCQTPKEKFIIVS"
     gene            complement(4507..4935)
                     /locus_tag="PLD44_06625"
     CDS             complement(4507..4935)
                     /locus_tag="PLD44_06625"
                     /inference="COORDINATES: protein motif:HMM:NF021505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HOF99044.1"
                     /translation="MFSIDHLHPMLVHFPIALIMVGFLFDLVSLLIKKEACLSFAGFW
                     LLIIGTLATIVTFFSGVFFTPDMAGTAGMVKNIHATFAVITMLLLIINSLLRIYLKRS
                     NRENSPMKWLSFAIYALAALMVGITGFYGGNLVYNYMMPL"
     gene            complement(5179..6039)
                     /locus_tag="PLD44_06630"
     CDS             complement(5179..6039)
                     /locus_tag="PLD44_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOF99045.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGREDLISVEEEVELAQRIH
                     KGDRAALEKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFSKFEQENERRPSPEELAE
                     ELDIPVDKIADTMKVSGRHISVDAPFVEGEDNSLLDVMVNEDSPDADHRLINESLSQE
                     IERALSTLTEREHEIIKKFFGIGVPEMTLEEIGDEFGLTRERVRQIKEKAIRRLRNSS
                     KSKLLKSYLG"
     gene            complement(6304..7785)
                     /locus_tag="PLD44_06635"
     CDS             complement(6304..7785)
                     /locus_tag="PLD44_06635"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOF99046.1"
                     /translation="MEASKRKKALGLFLLVVVVSVATNVTTNYFTNKHEKDNFKRELQ
                     YPTAYAKFSNVKSGVETDFTLAAELTIHSVVHVKTQSKAKMAFREDPFFEFFFGRPRY
                     YQEPPMVEGSGSGVIISNDGYIVTNNHVIDGSSKIEVTLNDKRTFEAKVIGADPNTDL
                     ALIKIDAENLPIIPFGNSDSLKVGEWVLAVGNPFNLNSTVTAGIVSAKARNINIINSK
                     MRIESFIQTDAAVNPGNSGGALVNTRGELVGINTAIASQTGSFAGYAFAIPVSIVKKV
                     VSDLRQYGVVQRAVLGVEIADITAQLAKEKDLKTLEGAYVANVNAGSAAEKAGIKEGD
                     VIVNVNGVAVKSVSELQEQIGRYRPGDKISITVIRKNNELKFNNVELKNSQGSASIVS
                     SDKVDVEQLLGATFKELSSKLQKDLGLDYGVQVTSLTKGKLMQQGVRVGYIILKINNQ
                     TIRNVEDIQSAVTAALNSEDKVLFMAGVYPNGRVTYYAVNLEE"
     gene            complement(8046..9335)
                     /gene="eno"
                     /locus_tag="PLD44_06640"
     CDS             complement(8046..9335)
                     /gene="eno"
                     /locus_tag="PLD44_06640"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOF99047.1"
                     /translation="MSRIEKIVAREVLDSRGNPTVEVDVMLESGVMGRAAVPSGASTG
                     ENEAIELRDGDKKRYLGKGVLKAVSNVNNIIAPSLIGISALDQRTIDKKMIELDGTKT
                     KSNLGANAILGVSLATAKAAAAYLDLPLYRYIGGVNAFVLPVPMMNIINGGSHSDAPI
                     AFQEFMIRPVGAPSFHEGLRMGAEVFHSLKKVLHDRNLSTAVGDEGGFAPTLKGTEDA
                     LESILTAIEKAGYKPGQDVMIGLDCASSEFYENGIYNYTKFEGANGAKRTSEEQVKYL
                     SELVSKYPIDFIEDGMSENDWEGWKLLTEKLGNKVQLVGDDLFVTNVEFLKKGIEMGC
                     ANSILIKVNQIGTLSETLDAIEMAHRAGYTTVTSHRSGETEDATIADIAVATNSGQIK
                     TGSLSRSDRMAKYNQLLRIEEELGDLAVYGYKKVTKK"
     gene            9506..10267
                     /locus_tag="PLD44_06645"
     CDS             9506..10267
                     /locus_tag="PLD44_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HOF99048.1"
                     /translation="MRTTKFILFSFICISLTSCNVLMQGVSNSGTLSIPTTDKENIAG
                     IKNALLLGTERAVSDLSIENGFYGNQAFKLLLPEEAQTIVENISLIPGGKALVDKAIL
                     SINRSAEDAVKEAGPVFKKAIQDMTISDAMGILLGGSNAATQYLQKTTSTELKNLFLP
                     KVQASLKKPLVGDVSTTETWNLLINAYNSAANSTLGKLASLKPVNTQLDQYVTDKALS
                     ALFTKMAETENSIRTDPAARTTTLLKRVFGQLDNK"
     gene            10409..10717
                     /locus_tag="PLD44_06650"
     CDS             10409..10717
                     /locus_tag="PLD44_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOF99049.1"
                     /translation="MENNENQINIELSAEVAEGIYSNLAIISHSSSEFVLDFIRIMPG
                     LPKAPVKSRIILTPEHAKRLLYALQENIAKYEQQNGKIKIDENSFIPPFPMGFGGGEA
                     "
     gene            complement(10760..>11644)
                     /locus_tag="PLD44_06655"
     CDS             complement(10760..>11644)
                     /locus_tag="PLD44_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOF99050.1"
                     /translation="GIYPAFDREKYLSGELAPVFFGSALNNFGVKELLNCFVEIAPSP
                     QPVQAHERVVNPYEETFSGFVFKIHANMDPNHRSCIAFVKICSGKFERNVNYKHIRQG
                     KMLRFSSPTAFMAQKKEIVDEAYSGDIVGLPDNGNFKIGDTLTGGEDLHFKGLPSFSP
                     EMFNYLENADPMKTKQLEKGIRQLMDEGVAQLFTSEFNRRKIVGTVGQLQFEVIQYRL
                     LHEYNAQCRWEPISLYKACWIESDDAEQLEAFKKRKAQFMVKDKEGRDVYLAESPYIL
                     QMAQRDFDHIKFHFSSEF"
BASE COUNT         3693 a         2351 c         2001 g         3599 t
ORIGIN      
        1 tctgaatatt atattcattc gtgtttgtgt atgcaataca tgtgggtttc ataaattaaa
       61 atttcattca aagttaaata aatcttctct aacaaatttc acatatttgg gttcagtcgg
      121 gatgtaattt tcatcgtccc acagcgtgct ggtaatcatc aaatcggcac tgtaacgatt
      181 gcaggcaatg ggaacattat gaatcctgca ctgacggagc aacatttgga tatcggcttc
      241 gtgtggctgt ggattcaaat catcaatcag aaaaactgcc agattaatct gtttttgtac
      301 caccaatgcg gcaatttccg cgtcgccgcc caaagggccg gagtgcatgc aagtaatatc
      361 caccttcaca cctttttctt ccatagccct ttgaatgagt tttcctgtcg taccggtaca
      421 aatcaatttg tgttgcgaaa gcatatcggc attgtaaaca gcccattcta ccatgtcggc
      481 ttttcgatta tcatgagcta ccagcgcaat tttcaattgt tttttcataa gttttatgtt
      541 ttttgatgaa agaatttatt aaagttttca atgaaaatct cagttgaagg ttgaatcatt
      601 ttttcgattc agaatcgatc gaattaatga aaaataaaaa attcaatcta taaaaacagg
      661 aatttcaaac attaatagaa ataaattttt tgctgttttc gaatgttttt tcagaaaaaa
      721 ccttataatc ctaatggttg caaaaataaa gaataaatat ttatttcgga caaggaaaat
      781 gaaaaaactt ttataataac atcatagcgc ataaaactca tttgattatt tcaagctgtt
      841 tatatctcgt taaattttta aacaaagctt tcaaccgaaa agtacttatg gtttcataaa
      901 aattgtcttt aaaaaccgat tcaatttgct aactttgcat aaagaaaacc ggtaagattt
      961 aaagtttcta caaaaatatt tggatgaaca aatcaatgaa tttggatttt ttaaacgatt
     1021 tgaacgaaag tcagcggcaa gctgtagaat atatggatgg cgcttcgctg gtaatagcgg
     1081 gtgcagggtc gggaaaaacc cgcgtgttaa cttacaaaat tgcctattta ctcaccaaag
     1141 gttttcctcc gtcgtccatt ttggctttaa ctttcaccaa caaagcggca cgagaaatga
     1201 aggaacggat tgcacaaatt gttggagaaa gaacagcccg atacttgtgg atgggaactt
     1261 ttcactccat tttttccagg attttgcgta acgaagcaca gtacaccgga tattcaaaaa
     1321 atttcaccat atacgactct tccgattctt ccagcttgat aaaaagcatt ctgaaagaac
     1381 gcaatctgga tacaaaaact tatccgcccg gactcattca atcacgcatt tcttcggcaa
     1441 aaaacaacct gattacaccc gaagcctatg ccgtcaacag cgattttatt aaaagtgaca
     1501 agcaaagccg cattccgctt ttgagcgaaa tttatcaaac ttattgcaat cgttgcaaac
     1561 aagccgatgc aatggatttc gacgatttac tgctccaaac taatctgctg tttcgaaatc
     1621 atcctgaagt gctggaaaaa tatcaacaat tgtttcaatt tattttggtt gacgagtatc
     1681 aggataccaa ctttgcgcaa tacctgatta taaaaaaact tgctcagcaa cacgaacgaa
     1741 ttagtgttgt gggtgacgat gcacaaagca tttattcatt tcgcggtgcc aacatcgaca
     1801 acattctaca gttcaagaat gcttatcaca atgcacgcat cttcaaactc gaacaaaatt
     1861 atcgttctac acaaaccatc gtgaatgcag ccaacagcct gattgacaaa aacagggcac
     1921 aaattcaaaa aaccattttt tcggaaaaag aaatcggaca tcccataaaa ttgttgaatg
     1981 ttttcacaga ttatgaagaa ggtctgaccg ttgccaaaca tattttggac ttgtacgcgc
     2041 tatacgatta tcgatatgcc gacattgctg tgttgtatcg aaccaattca caatcgcgaa
     2101 ttttagaaga aacattccga aaacagggca ttccctatcg aatttacgga ggcctgtcgt
     2161 tttatcagcg aaaggaaata aaggatgtga tagcttattg ccgattggtt tgcaaccaaa
     2221 atgatgaaga agcattgaaa agaatcatta attttcctac gcgaggcatt ggcgaaacta
     2281 cagtagggaa acttgtggaa tgtgcacaga gtaaagatgt aggaatttgg gaagttcttt
     2341 ccaacatgac aaaatacggc gtgccggtca atgcgggaac agcttcaaaa ttaaacgatt
     2401 tccgaaattt gatagaaagt ttttcccagc aactggaaat taaaaatgct tacgacttgg
     2461 ccgttatgat tgtaaaaacc accggattgc tcaacgaagc caactttgat cattctcctg
     2521 aaagtataag cagaaaagaa aatatagaag aatttctgaa agccatccac gaattttgtg
     2581 aaaaaaaaat ggaagaagga gaaaccttgc cccttcttcc cgatttttta tcggaagtat
     2641 ctttattgac cgaccaggac accgacaagg acagcctgag cgacaaagtt accctcatga
     2701 ctgttcatgc agccaaagga ttggaattta aagccgtatt cattgttggt atggaagaag
     2761 aacttttccc gtcaatgttc agccttgtca atgaacggga actggaagaa gagcgccgcc
     2821 ttttttacgt tgccattacc cgtgccaaag aacgctgttt tatcagctat gccaaatcga
     2881 gatttagaaa cggtaaaacc aacttttgca ctcccagcag atttatcaaa gatatcgacc
     2941 ctcagtttct ggaaattacc gaagaaaaag caccaacttt tcagaaaaaa aattttatga
     3001 attgggatga ggaaattgaa agtgaacgga atcgttttta ctcgccaaca aacgaaaaag
     3061 aaacttattc ggaacctcaa acaacaaatt tctctccacg aaaactgaaa aaattggatc
     3121 aatcgtcgga cgaaaaaatc attatcgaaa attcttcttt tcctgtagga actattgtaa
     3181 aacatcagct tttcggaatt ggaaaagtat tggaaaccag tatctccaac ggcaacgaaa
     3241 aagcaacgat cgattttgga gaaaaaggag taaaatcgct tttactgaaa ttcgcaaaac
     3301 tggaagtggt gaaataatta tcgaagagta tttaccgatg ccttaatggg ttaaatgttt
     3361 ttgcttaaac aaagatgatt gaactttcac attttgatag atgtggttag gcgtgttttc
     3421 tattaacatt cgtgaaaaat gaaaattttg tcgaaagacg aaatacgaaa catttgcata
     3481 atttcaaaaa ttgaaagcga actttttgtt taatttctgg gtctatttat tttttaaata
     3541 ctcgctacag atatctctat attggttaag ttgtcgggtt gtcccctttt ttcagttttt
     3601 agaaattact gtaaaggtgt gataatcaac cactcaacca tattttttta aattaaactt
     3661 ttttgtttaa tctatgatga tcaatttatt aattcattaa tttattgaag tattgtcaaa
     3721 aataagttgc aaaaaagttc gaatatgtga agagcccgat taagaccgga ctcttccaat
     3781 gaacaaaaaa cttataaacc aagatcaaag caaataatgg atggtttctt cgtccctgtt
     3841 attaatcaag aaacaataat aaatttctct tttggagttt gacaaaaagc acagtttgga
     3901 tcaatggctg cattatcgta agtatttcca caaacaggac aaactgcata agcagatggc
     3961 aatgtgcttt cattgtttgc ttttaaagcc gctaaagctt tctgataaaa atcgagatgt
     4021 ttcttctccg tatcaagagc ccacgtaaaa gatttcacgg catcggtaac tttctcggca
     4081 tttgcatcat taataaagtt aacgtacatt gtggtgcttt cataagattc tccatcaata
     4141 gcagcctgca aattttctgc cgtagattta acctcaaatg agggagtaaa agcttccatt
     4201 ttttctccaa gactttccaa tacttttgtg tgattggcag catgaatggc ttcggcctta
     4261 gaagctgcat caaaaagttt tgctatggta tcatagccct cttctctggc tttttgagca
     4321 aaagcggaat actttgcact cgcggtggtt tcacccttta ttccttcttt caagtttgct
     4381 atggtctttg taggggtaga tttacaaccg aaaaatgcaa ccgtaagcaa tgcaataaaa
     4441 ataattctat tcactttttc cataatgtaa aaatttgaaa atgttaaaca tattgtttat
     4501 gaaaatttag agaggcatca tataattata aaccagattt ccaccataga aacctgtaat
     4561 tcccaccata agggcagcca atgcataaat ggcgaatgac aaccatttca ttggagaatt
     4621 ttccctattt gatcgcttca aataaattct aagcaaagaa ttaattatca acaataacat
     4681 ggtaatgact gcaaaagtag catggatatt tttaaccatt cccgccgtac ctgccatgtc
     4741 gggtgtaaaa aagacaccgg aaaaaaaagt aacaatggta gccaaagtcc caattatcag
     4801 caaccaaaat ccggcaaagg ataaacatgc ttctttcttt atcagaaggg ataccaagtc
     4861 aaaaagaaac cccaccatta ttaatgcaat gggaaaatgt accaacatag ggtgcaaatg
     4921 atcgattgaa aacataactt aaaagttttt tggattgtaa gatattgaat gacacttgaa
     4981 aaaattgcgt tcaaatattt atagcgattt tactataatt tgaattttaa cactcacact
     5041 tttaaactaa ttacaaagat aaaacatttc taattaattt ttgttgaata acaaaaaaaa
     5101 tcaaatcttt ggaataaaaa aagcgtcctt tttggggacg ccctttgcat tcattttaaa
     5161 ataagcattt tatgaaaatt acccgaggta actcttcaat aatttacttt tagaactgtt
     5221 tctcaatcta cggatagctt tttccttgat ttgacgaaca cgctcgcgtg tcagtccaaa
     5281 ttcatcgccg atttcttcca acgtcatttc aggtacgcca atcccaaaga attttttaat
     5341 aatttcatgc tctctttctg ttaatgttga cagagccctt tcaatttcct gggaaagtga
     5401 ttcattgatc aaacgatggt cggcatcggg agaatcttca tttaccatca catcgagcag
     5461 gctgttgtct tccccttcaa caaaaggcgc atctacggaa atgtgacgac ctgaaacctt
     5521 catggtatcg gcaattttat ccaccggaat atcgagttct tcagccaatt cctcaggtga
     5581 aggacgacgc tcattttcct gttcaaattt tgaaaaagcc ttgttgattt tgttcaacga
     5641 acctacttgg tttaatggca aacgaacaat acgcgactgc tcggccaacg cctgcaaaat
     5701 ggattgcctg atccaccaca ctgcatagga aataaactta aatccgcgtg tttcatcgaa
     5761 tttttcggca gccttgataa gtccaagatt tccttcattg atcaaatcgg gcaaacttaa
     5821 gccctgattt tgatactgtt ttgctaccga aacgacaaaa cgcaaattgg cgcgggttaa
     5881 tttttccaaa gcggctcgat ctcccttgtg aatccgttga gccaactcaa cttcttcttc
     5941 cacactgatc agatcttctc ttccaatttc ttgcaagtac ttgtcgagcg atgcgctttc
     6001 gcgattcgtg attgattttg taatttttaa ttgcctcatt tttaccttaa aaaattaggg
     6061 tgcaaaatta atgttttttt ggttgattac aaaatttctt atataacgtt tttactaaat
     6121 ttttgtttta aaaagtcttg aaaatttttc tttgttgttc cataaatacg ccgaaacgat
     6181 attttatgag ttgcctgatt gttgataatc tcaaatttta gtacaaaagg aacttactcc
     6241 tttcgtaaaa aagagtaagt tccaatgcaa cagagaaata tttttcaaca cacttatgga
     6301 aaattattct tccaaattaa ctgcatagta ggtaactctg ccattgggat aaacacccgc
     6361 catgaaaagc accttgtctt cgctgtttaa tgccgcagtt acggcagatt gaatatcctc
     6421 cacatttcta atagtctgat tgttaatttt taatataata tatccaactc tcacaccttg
     6481 ctgcatcaat tttccttttg ttagcgatgt aacctgaaca ccataatcca aaccaagatc
     6541 tttttgcaac ttactgctca attccttgaa agtagcaccc agaagttgtt caacgtcaac
     6601 tttgtcggaa gaaactatgc tggcacttcc ctgactattt ttcagttcta cgttgttaaa
     6661 tttcaattca ttatttttac gtataacggt gattgaaatt ttatcgccgg ggcgatagcg
     6721 tccaatttgt tcctgcaatt ccgaaacaga tttaacagcc acgccattca cattcacaat
     6781 tacatcacct tctttaatgc ctgctttttc ggcagcactt ccggcattta cattggccac
     6841 ataagctcct tccaatgttt tcaaatcttt ttcttttgcc aactgtgcgg taatatcagc
     6901 tatttcaact cccaacacgg ctcgttgtac tactccgtac tgacgcaaat cggaaactac
     6961 ttttttaact atgctgaccg ggatggcaaa tgcgtaacct gcaaacgaac ccgtctgaga
     7021 agctattgcc gtattgatac ctaccaattc gccccgtgta ttgaccaaag cgcctccact
     7081 attacctgga ttaaccgcag catcggtctg aataaaagat tcgatacgca ttttggagtt
     7141 aatgatattg atatttcttg cctttgcact cacaattccc gccgtaacgg tagaattaag
     7201 attgaaagga ttccccactg ccagcaccca ttcgccaact tttagcgaat cggaattacc
     7261 aaaaggaatg atgggaagat tttctgcatc gatttttatc agcgccaagt ctgtattagg
     7321 atcggcacca attacttttg cctcaaatgt tcgtttatcg tttaatgtaa cttctatttt
     7381 ggacgaaccg tcaattacat ggttgttggt tacaatatat ccatcattgg aaattatgac
     7441 gccagagcca gaaccttcta ccataggcgg ttcttgataa tagcgaggac ggccaaagaa
     7501 aaattcaaaa aagggatctt ccctaaaagc catttttgct tttgattgtg tttttacgtg
     7561 tactaccgaa tgaatcgtaa gttcagctgc caatgtaaaa tcggtttcca caccactctt
     7621 cacattggaa aattttgcat aagctgtcgg atactgcaat tctcgtttaa aattatcttt
     7681 ctcatgttta ttggtaaagt aattggttgt cacatttgtt gccacactca ctactaccac
     7741 caacaagaac aggcctaacg ctttttttct tttcgatgct tccatacttt tcgtttttaa
     7801 gatttaaatt gatttttctg aaaaaatatt cttaaacaaa tttttcactg ctaaagtata
     7861 caaaatacat tccataacac aaagtgatat agctatttgt cgaaatttaa cacagagatt
     7921 tgcagcttaa cgggaattaa cagaaagacc ttagattttt tagaaaaatt ttgttactaa
     7981 aataaaaaac cacctttttg acaggtggtt ctggattgaa aacaaaaaac tgacaattta
     8041 tactgtcatt ttttggtaac ttttttgtag ccataaacgg ccaagtctcc caattcttct
     8101 tcgatacgaa gcaactggtt atatttggcc atacggtctg aacggctcaa agaacctgtt
     8161 ttgatctgac cggaattggt tgctactgcg atgtcggcaa tggtagcatc ttccgtttca
     8221 cccgaacgat gagaagttac cgttgtatat ccggcacgat gagccatttc gatagcatcc
     8281 aaagtttcgg acaatgttcc aatttgattt accttgataa gaatggaatt ggcacaaccc
     8341 atttcgatac cttttttcaa aaattctaca ttggtaacaa acaaatcgtc cccaaccaac
     8401 tgaaccttgt ttccaagttt ttcggtcaat agtttccagc cttcccagtc gttttcactc
     8461 ataccatctt caataaaatc aatcggatat ttgctgacca attctgatag gtatttaacc
     8521 tgttcttcag aagtacgttt ggcaccattt gctccttcaa atttggtgta attataaata
     8581 ccgttttcgt aaaattctga cgaagcacaa tccaacccaa tcatcacatc ctgtccgggc
     8641 ttgtaaccgg ctttttcgat tgcagttaaa atagattcca gcgcatcttc tgtccctttc
     8701 aaagttgggg caaatccgcc ttcatcacca acagctgtgc tcaaattacg gtcgtgcaac
     8761 acttttttca gtgaatgaaa cacttcggct cccattcgta aaccttcgtg gaaagaagga
     8821 gcaccaaccg ggcgaatcat aaattcctgg aacgcgatgg gagcatcaga atgcgaacct
     8881 ccattgataa tgttcatcat tggaaccggc aaaacaaaag catttacacc accaatatat
     8941 cggtaaagcg gcagatcgag ataagctgca gcagcttttg cagtagccaa agaaactccc
     9001 aaaatggcat ttgcacccaa attcgacttt gttttcgtac catcgagctc aatcattttc
     9061 ttgtcaatag tacgctgatc caatgcagaa ataccaatta atgaaggtgc aataatgtta
     9121 tttacgttcg aaacggcttt cagtacgcct tttcccagat agcgtttttt atcgccatca
     9181 cgcaactcaa tggcttcatt ttctcccgta gaagctccgg acggaacagc agctcttccc
     9241 ataacgcccg attcgagcat tacatctact tctacagttg gatttcctct tgaatccaat
     9301 acttcgcgag caacgatttt ttctattcta ctcattttct ttataattaa gattgataat
     9361 ttgttagaaa ttcctctttt tacattttct gcaaagttat aaaatgttct tttaattttg
     9421 tttatatacg tacttaaaat atcaaaaaat agcgtatatt taccttctta aaaatgaaaa
     9481 ttaaatattt tgcaacaata aagcgatgag aacaacaaaa tttattttat ttagcttcat
     9541 ttgtatttcc cttacttcat gtaatgttct tatgcagggt gtttccaatt caggaacatt
     9601 atctattcca accaccgaca aagaaaacat tgcgggtata aaaaatgctt tgttattggg
     9661 aacggaaaga gcggtatccg atttaagtat agaaaatggc ttttatggta atcaggcatt
     9721 taaattactg ttgcctgaag aagctcaaac gattgtagaa aatatcagtt tgatacccgg
     9781 aggcaaagct ttggtcgaca aagccattct gtccatcaat cgttcggcag aagatgcagt
     9841 aaaagaagca ggtcctgtgt ttaaaaaagc gattcaggac atgactattt cagatgcaat
     9901 gggtattttg ctgggcggaa gcaatgcagc aacgcaatat ttgcaaaaaa ctacttcaac
     9961 ggaattgaaa aatctttttc tacctaaagt tcaggcatcg ctaaagaaac cattggtagg
    10021 tgatgtttca actacagaaa catggaattt gctgataaat gcctacaaca gcgcagccaa
    10081 ttctactttg ggtaaacttg cttctctgaa acctgttaac acccaattgg atcaatatgt
    10141 taccgacaag gcattgagtg cattgtttac taaaatggca gaaacagaaa attccattcg
    10201 cacggaccct gcagcccgca ctacaacatt actgaaacgc gttttcggac aactggacaa
    10261 taaataattc atttgctgga ttatacaatt ttttctctct ttagtacata gaatgctaaa
    10321 atagtttctt cacacacata aatattttgc tacttttgca acgcggtttc aacaaaggtg
    10381 aaaaataaat taattaaaat ataagacaat ggaaaacaat gaaaatcaaa tcaatataga
    10441 actttctgct gaagtggcag aaggtatcta ttccaatctg gctattattt cacattcttc
    10501 ttccgaattt gtgctcgatt tcattcgcat tatgcccgga ttacccaaag ctccggtcaa
    10561 atcacgcatc attctcaccc ccgaacatgc caaaagatta ttgtatgcac tgcaggagaa
    10621 tattgcaaaa tatgaacagc aaaacggaaa aataaaaatt gatgaaaatt cttttattcc
    10681 cccttttcct atgggatttg gaggcggaga agcctaattt tttctacgca aattccatct
    10741 ttcttagttg aaatagaaat taaaattcgg aagaaaaatg gaatttgata tgatcaaaat
    10801 cgcgttgagc catttgcaaa atatagggcg actcggcgag atatacatcg cggccttctt
    10861 tgtctttcac cataaattgc gcttttcgtt tcttgaaggc ttcgagctgt tcagcatcat
    10921 cgctttctat ccaacacgcc ttatataagg aaatcggctc ccaacggcat tgcgcgttgt
    10981 attcgtgcaa caatcggtat tgaattactt caaactgcaa ctgcccgact gttcccacta
    11041 tttttcttcg attaaattcg ctggtaaaaa gctgtgctac gccttcgtcc atcaattgcc
    11101 gaatcccttt ttccaattgt ttggttttca tcggatcggc attttcgaga taattgaaca
    11161 tttcgggcga aaaactcggt aatcctttaa aatggagatc ttctccaccg gtcagcgtat
    11221 cgccaatttt aaaatttcca ttatcgggca atcccacaat gtcgccggag taagcttcgt
    11281 ccacaatttc ctttttttgt gccataaaag cagttggact tgaaaaacgc aacatttttc
    11341 cctgtcgaat atgcttgtaa ttgacatttc gttcaaattt tcccgaacaa attttcacaa
    11401 aagcaataca actccgatga ttgggatcca tattggcgtg aatcttgaaa acaaaacccg
    11461 aaaaagtttc ctcgtaagga tttacaactc tctcgtgagc ctgaacgggt tgcggagaag
    11521 gagctatttc cacgaagcaa ttcaacaatt ccttcactcc gaaattgttc aaagcgctgc
    11581 caaaaaaaac cggagccaac tctcccgaca gatatttttc cctgtcgaaa gccggatata
    11641 ttcc
//
LOCUS       DAOSIB010000067        11276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_5706_length_11276_cov_11.840567, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000067 DAOSIB010000000
VERSION     DAOSIB010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11276
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5706_length_11276_cov_11.840567"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..1274
                     /gene="metF"
                     /locus_tag="PLD44_06660"
     CDS             324..1274
                     /gene="metF"
                     /locus_tag="PLD44_06660"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOF99051.1"
                     /translation="MSIVDKIKSSSSTAFSFELLPPLKGDGIASVFKTIDLLREFDPK
                     YINITTHRSEVIFKQNDQGLFEQSDVRRRPGTVAIAAAIKSKYKIPVVPHVICSGFTK
                     EEIEYELIDLQFLGITDLLLLRGDKAKHEKDFVPTGHMHATDLQQQVNNFNKGIFLDG
                     SRMKALPAEPFSYGMAAYPERHEESPNTESDLYYTKMKVDGGAEYLVTQMFFDNQKYY
                     DFVDKCRNMGITVPIIPGIKPITLMNQLTVLPKIFHTEIPEEFASELRKCKTDAEATE
                     VGVEWCTKQASDLKAHGVPSIHFYSLMAAQSVKRVAKAVF"
     gene            1293..1526
                     /locus_tag="PLD44_06665"
     CDS             1293..1526
                     /locus_tag="PLD44_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4834 family protein"
                     /protein_id="HOF99052.1"
                     /translation="MHLIFSILILIVFIGVLFLLFVLGFIRSFFNFGRKSKKRKQSKE
                     DNNIFTHSSKKKKIFDKNDGEYVDFEEIEEETK"
     gene            complement(1883..2611)
                     /locus_tag="PLD44_06670"
     CDS             complement(1883..2611)
                     /locus_tag="PLD44_06670"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOF99053.1"
                     /translation="MTVFYAPEVAFNNVLPKEESQHAIKVLRLKKGDKIVIVDGKGNG
                     YLAEILFPDSNHCEFEILETLPDEGNVHYHLHIAIAPTKNVERFEWFVEKATEIGIHE
                     ITPIICKFSERKMIKPERLEKIVISACKQSMRAQFPVVHSLCSFQEVLTTYSAQQKFI
                     AHCYTDQIMKEFDCVRKRNFLKNLIEPSTKIFILIGPEGDFSIDEVKTAIEKGFDPVS
                     LGVGRLRTETAGIVACTTVALINQ"
     gene            complement(2695..3588)
                     /gene="folD"
                     /locus_tag="PLD44_06675"
     CDS             complement(2695..3588)
                     /gene="folD"
                     /locus_tag="PLD44_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOF99054.1"
                     /translation="MYTLLDGKLISEQIKKEIAEEVQQMIKAGKKRPHLAVIIVGHDG
                     GSESYVAGKIKDCQICGFQSTLIRLEEDVTEQRLLQEVERLNEDTDVDGFIVQLPLPK
                     HISEQKMIEAINYKKDVDGFHPINVGRLCIGLPCYVSATPMGIMELLKRYRIETSGKN
                     CVVVGRSNIVGKPTAMLLMQKTYPGDATVTICHSHTKNLKEICQQADIIVAAIGQPEF
                     LKGDMVKEGAVVIDVGTTRVPSTETKSGFKLKGDVDFNEVAPKCSFITPVPGGVGLMT
                     RVSLMKNTLLAAKAEIYEKNG"
     gene            complement(3842..5266)
                     /gene="ahcY"
                     /locus_tag="PLD44_06680"
     CDS             complement(3842..5266)
                     /gene="ahcY"
                     /locus_tag="PLD44_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOF99055.1"
                     /translation="MTDNKALFTSLPYKVADISLADFGRKEIELAEKEMPGLMAIREK
                     YAAQQPLKGARITGSLHMTIQTAVLIETLAKLGAEVRWCSCNIYSTQDHAAAAIAAAG
                     ISVFAWKGETLEDYWWCTLQALTFEGGKGPNLIVDDGGDATLMIHTGVAAEKDANVLN
                     RPAEGEDEKELLAILKKVLKENSNFWSKITPEIRGVSEETTTGVHRLYQMLAEGTLLF
                     PAFNVNDSVTKSKFDNLYGCRESLADGIKRATDIMLAGKVVVVCGYGDVGKGCAKSMR
                     AYGARVLVTEIDPICALQAAMEGFEVCTVEDALPEGNIYVTATGNRDIITIQHLEKMK
                     DGSIVCNIGHFDNEIQVDQLKKFPGIQKVNIKPQVDKYIFPDGHSVLLLADGRLVNLG
                     CATGHPSFVMSNSFTNQTLAQIELFTKKYEINVYRLPKHLDEEVARLHLGQLGVKLTQ
                     LTKEQAEYIGVNPEGPFKPEHYRY"
     gene            5400..6038
                     /locus_tag="PLD44_06685"
     CDS             5400..6038
                     /locus_tag="PLD44_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOF99056.1"
                     /translation="MNIFVDIHTHGKSEFNPYSVRSLQIKEAALFFDAEERRFCSVGI
                     HPWNAEQDNLQDLERFFRYSSDTRWIAIGECGLDKNISTPFATQKSIFEGQISYSESL
                     KKPLIIHCVGYFNELLEIRNKFRPEQLWIVHGFRGKPELAKQLLKTGISLSFGEKRNA
                     ASVAVTPIDRLYVETDESLLPVEQFYREIAEIKNCRPENLNAGKKLLHLKNI"
     gene            6158..7741
                     /locus_tag="PLD44_06690"
     CDS             6158..7741
                     /locus_tag="PLD44_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOF99057.1"
                     /translation="MKKLYLVFIFNLILAGVFGQGISLMQQRKLMNALNAISSLYVDS
                     INDKKLIESAITALLKDLDPHSSYIPREEVERINEPLEGEFEGIGIQFQIFEDTLMVV
                     QTIAGCPAEKVGILPGDRIIYINDELVAGVKIQNSDILKRLRGPRGSEVTVKVLRRGK
                     PELLIFKIIRDKIPLYSVDAQYMIGNDIGYLKINSFGRTTDEEFVKAFTELQKKGMKH
                     LIVSLQGNGGGYLATAIQLADQFLDRGKLIVYTEGFHQPKKESLATAVGKYETGKLVI
                     LVDEYSASASEIVSGALQDWDRAVIVGRRTFGKGLVQQQLPLVDGSMMRLTTARYYTP
                     SGRCIQKPYKDGADKYERELLTRYKKGELIHPDSIHFPDSLRYQTLRLKRTVYGGGGI
                     MPDVFVPLDTTRFTDYHQKLVARGVINKVSVQFIDQNREDLKRKFSTFDKFEKEFTVD
                     EAFLQQLIQAGEKENIPFQSSQFDTSRELIKLQLKALIARDLWDMNAYYRIINNDNEV
                     LKKAVELLQTPNAYEQILK"
     gene            7755..8378
                     /gene="pnuC"
                     /locus_tag="PLD44_06695"
     CDS             7755..8378
                     /gene="pnuC"
                     /locus_tag="PLD44_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOF99058.1"
                     /translation="MILLKETFSLNPVIEYVIDNWVELTGAILSLIYLILSVRQKIGL
                     WIFGFLCAALYVYVFFQSKFYAGMTLQFYYLAVSVYGWITWKRGKTDTQPELPVTRTS
                     KHMAFNLLTGFLAILVIYFFILSNFTDSPLPRGDAFTTALSIVATWMLAKKLLENWLL
                     WIVADAVSTGLYLYRGLYPTAILFTVYTLMAVLGYFQWKKSMKHQTH"
     gene            8395..8931
                     /locus_tag="PLD44_06700"
     CDS             8395..8931
                     /locus_tag="PLD44_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF99059.1"
                     /translation="MKNMLRVAILGPESTGKTELAKSLAEYFQVDWVPEFARAYVESL
                     SRPYEYEDVCRIAAHQIDEELFYENHPSDGKKFVFFDTDLIITKVWFDYKYGKVPKFL
                     QERLNCHFFDFYLLCYPDLPWQPDSVREHGDDREFFFNWYKQEIKKTGKSVSIITGIG
                     NERLQNAIRALTSFVRYL"
     gene            8950..9840
                     /locus_tag="PLD44_06705"
     CDS             8950..9840
                     /locus_tag="PLD44_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOF99060.1"
                     /translation="MEKEILDAIIELSKIDQYKFACHQHVRNERMPSINNLQEIVQLC
                     RSVLFPGYFDDAALDSDTLLYHIGLSIDRLKLLLTEQLKAGFCFEREHSTDKNLCSDM
                     EQNVVQKVNAFIAQLSEIRRKLYTDVIAAYNGDPAAKSIGEVIFCYPGIRAISNYRIA
                     HALLKLDVPLIPRIISELAHSETGIDIHPGAEIGDYFMIDHGTGVVIGETSVIGNNVK
                     MYQGVTLGARSFPFDENGNPIKGIVRHPRIGNNVIIYSNSTILGNITVGDGAVIGGNL
                     WVDSDVPAGAKLSQDDKLKR"
     gene            9851..11059
                     /locus_tag="PLD44_06710"
     CDS             9851..11059
                     /locus_tag="PLD44_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOF99061.1"
                     /translation="MKITSGNDNKIRKFFAEKEFPMIAKTFQGLEEVLAEELTELGAN
                     NVQIERRAVSFSGDKAMLYKANLHCRTALRILKPIFSFEADNTDELYKKIKSVEWDAL
                     LHVNNTFSIDTTVFSDEFQHSRFVTYRVKDAIVDWFSENFNDRPSVSIEDPDFVFNIH
                     IAGSHCTLSLDSSGESLHKRGYRTGQTKAPLNEVLAAGMLMLAGWKGQSDFLDPMCGS
                     GTLLIEAALIALNIPPGIFRSSFAFEKWPDFDKVLFDDLYNDDSQELLFEHHIFGSDI
                     SVSAIKIAQQNVKSSGLSKYISLKVLPLEKLEPPSSDCFIVTNPPYGERLPSKDIFEL
                     YANLGSLLKRKFSGCTAWVISSNEEALKKIGLKPSKRYRLMNGPLECWFQQYELFTGK
                     RNDYLQKKNS"
     gene            11185..11268
                     /locus_tag="PLD44_06715"
     tRNA            11185..11268
                     /locus_tag="PLD44_06715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11219..11221,aa:Leu,seq:caa)
BASE COUNT         3300 a         2136 c         2287 g         3553 t
ORIGIN      
        1 gtttttctaa aatttttttg ccttgttcgt ccaaaatgca tgcaaatatg ctatctttgt
       61 gaacgttaag gcaacatgct ttttccataa aattaccgga ttaaattgtt caacaattag
      121 tttatttcat tggtcgttaa aaaaccaatt tacaaaagca acatttgtgc ttcgagcacg
      181 gtacggtaaa cctttcaagt tttgtggcct tgatttttat tttcacacgt ttggaaatac
      241 ctcgattttc atgtaatttt gcaaattaaa tttctttatt cctcgttttt ttattgtaag
      301 tttcttacgt ttccttttaa gatatgagta ttgtcgacaa aataaaaagt tcttcttcca
      361 cagcattttc tttcgagctt ttgcctccat tgaaagggga tggcatagct tccgtattta
      421 aaaccatcga tttactaaga gaattcgatc ctaaatatat caatattaca acgcatcgga
      481 gcgaagttat tttcaaacaa aacgatcagg gacttttcga acaatcagat gttcgtcggc
      541 gtcctgggac ggtcgctatt gccgctgcca taaaaagtaa atataaaatt ccggttgttc
      601 cgcatgtgat atgcagtgga tttacaaagg aagaaattga atatgaactt attgatttgc
      661 aatttttggg aatcactgac cttttgctgc tcagaggaga taaagccaag catgagaagg
      721 attttgttcc aacgggacat atgcatgcca ccgatcttca gcaacaagtc aacaacttca
      781 acaagggcat ttttctggat ggcagcagaa tgaaagcact tcccgcagag cctttttcct
      841 atggaatggc agcctatcct gaaagacatg aagaatcgcc caacaccgaa tcggacttgt
      901 attatactaa aatgaaagtg gatggcggag ccgaatatct ggttacccaa atgtttttcg
      961 acaaccaaaa gtattacgat tttgtagata agtgcagaaa catggggatt accgttccta
     1021 ttattccagg tataaaacca ataactttaa tgaatcagct cacagtgctg cctaaaattt
     1081 ttcacaccga aattcctgaa gagtttgcgt ctgaacttcg caagtgtaaa accgatgcag
     1141 aggcaaccga agtgggggtg gaatggtgta ccaagcaggc ctccgattta aaggctcatg
     1201 gtgtaccaag tattcatttt tacagcttaa tggctgcaca gagtgttaag cgtgttgcta
     1261 aagccgtttt ttaaaatatt caatctctat ttatgcatct tattttttcc atactgattc
     1321 tcattgtctt tattggagta cttttccttc tttttgtgtt gggattcatt cggtcgtttt
     1381 ttaattttgg gagaaaaagt aaaaaaagaa aacaatctaa ggaagataac aacattttta
     1441 cgcattcatc aaagaagaaa aaaattttcg acaaaaacga tggagaatat gttgattttg
     1501 aagaaattga agaagaaaca aagtaagtgg ataaatccag aaaaaatttg ttccgataaa
     1561 tttctctcac atttgggcat gggctacaaa tagtttcgtc cttaagagat ttactcatta
     1621 gccaattcaa agggaaagtc aaagtcggaa gagttgttcg atagtcagga aaaccaagct
     1681 cgttttttta ttgaaagcaa ttttgttaaa cagccctgct gatttttttt taatatccac
     1741 cccctggaga agttctgcat aaaagtttta tagggtagat aaagttttat ctgttttata
     1801 caagattaaa acctttttcc tattgtggag ttgggtttaa ttgcttgaat ttacgagaga
     1861 atataattgt cccaatataa atttattgat tgataagcgc tacggtcgtg caggccacaa
     1921 ttccagctgt ctctgttctt aaacgcccca ctccgagaga aacaggatcg aatccttttt
     1981 cgatggctgt ttttacttcg tcgatactga agtcgccttc aggaccaatc aaaataaata
     2041 ttttcgtgga tggttctatc agatttttta aaaaatttct ctttctcaca cagtcaaatt
     2101 ctttcattat ctggtcggta taacagtgtg ctatgaattt ttgctgtgcc gagtaggttg
     2161 tcagtacttc ctgaaaactg caaagggaat gaacgacagg aaattgtgct ctcattgatt
     2221 gcttgcaggc agaaataaca attttctcaa gtcgttcggg cttaatcatc tttcgctcgg
     2281 aaaacttaca aataataggg gtaatttcat gtatccctat ttcggttgct ttctcaacaa
     2341 accactcaaa acgctcgaca tttttagtgg gagctatggc aatgtgtaaa tgataatgaa
     2401 cgtttccttc atcaggcaaa gtctcaagaa tttcaaattc gcaatggtta ctgtctggga
     2461 aaagaatttc agcaaggtag ccattcccct ttccatcaac tatcactatt ttatctcctt
     2521 tttttaagcg aagaactttt atggcatgtt gtgattcttc tttgggtaaa acattgttaa
     2581 aagcaacttc gggagcataa aaaacggtca taattatcaa aaagtaacga taaaatttat
     2641 tgtgtcaaaa caggcaatat aagttgaacc aaatttatcc gaaaaaaatt atatttatcc
     2701 atttttctca taaatttcgg ctttggcagc caacaaggtg tttttcataa gcgaaacgcg
     2761 agtcattaat cctacacctc ccggcacagg agtaataaat gaacattttg gagctacttc
     2821 attgaaatct acatctcctt ttagtttgaa acccgatttt gtttcggttg agggaactct
     2881 tgttgttcca acatcaataa ccactgctcc ttcttttacc atatccccct ttaaaaactc
     2941 tggttgccct atggctgcca cgataatatc ggcttgttgg caaatttctt tcaagttttt
     3001 ggtatgactg tggcaaatcg taacggttgc atcacccgga taagtttttt gcatcaaaag
     3061 catggcagta ggcttaccta ctatgttact gcgcccaaca acaacacagt tttttccact
     3121 ggtttctatc cgataacgct tcaataattc cataattccc attggcgtgg ccgaaacata
     3181 acacggtaat cctatacata atcggccaac attgatcgga tgaaaaccat ctacatcttt
     3241 tttataatta attgcttcaa tcattttttg ttccgaaata tgtttcggta aaggtaattg
     3301 aacaatgaat ccatcaacat cagtatcttc atttaaacgt tccacttctt gcagcaaacg
     3361 ttgttctgtt acatcttctt ctaatcgaat cagagtggac tgaaatccgc agatttggca
     3421 atctttgatc tttccggcta catacgattc acttccacca tcatgaccaa caataatcac
     3481 tgccagatga ggacgctttt taccggcttt tatcatttgt tgaacttctt cagcaatttc
     3541 cttttttatc tgttccgaaa ttagttttcc atccaaaagg gtatacatgt tataacttga
     3601 ttaaatgttt ttaggcatat caatttcatc agaatataat ttatttttta aaggtctgat
     3661 ggaacccacc tgtctgaata ttatattcat tcgtgtttgt gtatgcaata catgtgggtt
     3721 tcttattttt acggttttta gtttgtttgt agaaaaaggt aattgagtta aaaagaatga
     3781 attgaaaata aaggaaaaca acaatatctt cgtggaaaat acgattaatt ttttaaacat
     3841 ttcagtaacg gtaatgttcc ggtttaaaag gtccttcggg attcacacca atatattctg
     3901 cctgctcttt ggtaagttgg gtaagcttta ctcccaattg gcctaaatgc agtcgggcaa
     3961 cttcttcgtc caaatgttta ggtaaacgat aaacatttat ttcgtatttt tttgtaaaca
     4021 gctcaatttg agccagtgtt tggttggtaa aggaattgct catgacgaaa gaaggatgac
     4081 ctgttgcaca gccaagattt acaagccgtc cgtcggcaag aagcaaaaca ctgtgtccat
     4141 cggggaaaat atacttatcc acctgcggtt tgatatttac tttctgaatg ccaggaaatt
     4201 ttttcaattg atccacctga atttcgttgt cgaaatgtcc gatattgcac acaatggagc
     4261 catccttcat tttttccaaa tgctgaatgg tgataatatc gcgattgccg gtggcagtaa
     4321 cataaatatt cccttcaggc aaagcatctt ctaccgtaca cacttcaaat ccttccattg
     4381 cagcttgcaa agcacaaatc gggtcaattt cggttaccag cactcttgct ccataagccc
     4441 gcatggattt ggcacaacct ttccccacat caccataacc acaaactacc acaactttgc
     4501 ctgccagcat gatatctgtt gcccttttta tcccgtcggc caaagattca cggcagccat
     4561 aaagattatc gaatttcgat ttggtaaccg agtcgttgac attgaaagcg gggaacagca
     4621 atgttccttc cgcaagcatc tgataaagcc gatgaacacc cgtagtggtt tcttccgaaa
     4681 cgccacgaat ttccggagtt attttcgacc agaagttgct attttccttt aaaacttttt
     4741 tcaatatagc caacaattct ttttcatctt ctccttcggc aggccggttc aatacgtttg
     4801 catctttctc tgctgccaca cctgtatgaa tcataagcgt tgcatcgccg ccatcatcaa
     4861 caattaaatt cgggccttta ccgccttcaa aagttaaagc ctgcaaggtg caccaccagt
     4921 aatcttccag tgtttcgcct ttccaagcaa aaacagatat tccggctgct gcgatggctg
     4981 cagcggcatg atcctgcgtg gaataaatat tgcaactgca ccagcgtact tcagcgccaa
     5041 gttttgctaa cgtttcaatt aaaacagccg tctggatagt catgtgaagt gagccggtaa
     5101 tgcgggcacc tttcagtggc tgctgggctg catatttttc tcgaattgcc ataagtcccg
     5161 gcatttcttt ttctgccagt tctatttcct ttctgccaaa atcggccaac gaaatatcgg
     5221 ccaccttgta cggcaaagaa gtgaacaatg ctttattgtc tgtcattttg ttgattcttt
     5281 tgttcaaata tcctttaaat ctaatgaaaa gtttgcaaag atatgaattt tacttgaaaa
     5341 ttctttactt ttgcatcaat gttttcaaag gtagccgcag ttcatagagg ccgataaaga
     5401 tgaacatttt tgttgatatc catacacatg ggaaatctga atttaatccg tattctgtga
     5461 gaagtttaca gattaaggag gcagctcttt tttttgatgc tgaagagcgg cggttttgtt
     5521 cggtgggtat tcatccttgg aatgcagaac aggataatct tcaagatttg gaaaggtttt
     5581 ttcgttattc ttccgataca cggtggatag cgataggtga atgtggactc gacaaaaata
     5641 tttccactcc ttttgccacg cagaaaagca tttttgaagg tcaaatttct tattccgaaa
     5701 gtttgaaaaa acctttgatt atccattgtg tcggttattt caacgagttg ttggaaatta
     5761 ggaacaagtt tcgacccgaa caactttgga ttgtgcatgg cttccgcgga aagcccgaat
     5821 tggccaagca attattaaaa accggaattt ctctttcatt tggcgaaaaa cggaatgcgg
     5881 caagcgtggc agtaacgcca attgacagac tttatgtgga aacagacgaa agtttattgc
     5941 cggttgaaca gttttatcga gaaattgcag aaattaaaaa ttgtcggccc gaaaatttga
     6001 atgcgggaaa gaaattgctt catttgaaga atatttgaat cgattcagct aaagttgcaa
     6061 ttttcaaaga gataaaccga aaaaaattaa ttctttgaaa caaaaattgt ttgattatta
     6121 ttgttctctt ttttacttat gacataacca acaaaaaatg aaaaagttat atttggtttt
     6181 tattttcaat ttaattcttg ccggagtttt cgggcagggc atatcgttga tgcagcaacg
     6241 aaaattaatg aatgccttga atgccatatc cagcttgtat gtggacagca ttaacgataa
     6301 aaaacttatc gaatcggcca taactgccct gttgaaggac ttggatccgc attcatccta
     6361 cattccacgt gaagaagtgg agcgtatcaa tgaaccgctg gaaggcgagt ttgaaggaat
     6421 aggcattcag tttcaaattt ttgaagacac cttgatggtg gttcaaacta ttgcaggatg
     6481 tccggccgaa aaagtaggaa ttcttcccgg cgaccgaatt atttatatta acgacgaatt
     6541 ggtggcgggt gtaaaaattc aaaattccga cattttgaaa cgcttgcgtg ggccgcgagg
     6601 ttcggaagtt acagtcaaag ttttgcggcg tggaaaaccc gaattgctca tcttcaaaat
     6661 tatacgcgat aaaattcccc tctacagcgt ggatgctcag tatatgattg ggaatgatat
     6721 aggatacctc aaaatcaaca gttttggtcg gacaaccgat gaagaatttg tcaaggcatt
     6781 taccgaactt cagaaaaaag gaatgaagca tttgattgtc agcttgcagg ggaatggggg
     6841 cggttatctg gctacggcta ttcaactggc tgatcaattt ttggatcgtg gaaaacttat
     6901 cgtttacaca gagggattcc atcaacccaa gaaagagtcg cttgctaccg ccgtaggaaa
     6961 atacgaaacc ggaaaactgg ttattctggt tgacgaatac tcggcttcag ccagcgaaat
     7021 cgtttcggga gctttgcagg attgggacag agcagtcatt gttggacgaa gaactttcgg
     7081 caaaggattg gtgcagcaac agcttccgtt ggttgatggc tcgatgatga ggttgacgac
     7141 agcacgatat tatacgccat cggggcgttg cattcagaaa ccttataaag atggtgctga
     7201 taaatatgag cgcgaattgc tgacccgtta caaaaaagga gaattaatcc atcccgacag
     7261 cattcatttc cccgattcac tccgttatca gactttgcga cttaaacgta ccgtttatgg
     7321 cggtggaggt attatgcccg atgttttcgt tccgctggat accacacgtt ttaccgatta
     7381 tcatcaaaag ttggttgcaa ggggagtaat caataaagtt tccgttcagt ttatcgatca
     7441 gaacagggag gatttgaaga gaaaatttag tacgttcgac aaatttgaaa aggaattcac
     7501 ggttgacgaa gcctttttgc agcaactgat tcaggccgga gaaaaggaaa atattccgtt
     7561 ccagtcctct cagtttgaca cgtcaagaga gctgatcaaa ctgcaactta aggcattgat
     7621 tgcacgcgat ttgtgggata tgaacgccta ttaccgcatt ataaacaacg acaacgaagt
     7681 gctgaaaaaa gcagtcgaat tattgcagac gccgaatgcg tacgaacaaa ttttaaagta
     7741 aaaaggtcaa agcaatgatt ttgttgaaag aaactttttc gttgaatcct gtcatagaat
     7801 atgttatcga caattgggta gaacttaccg gtgctatttt aagcctgata tatctgatat
     7861 tatcggtgag gcagaaaatc ggattgtgga ttttcggatt tttgtgtgcg gcattgtatg
     7921 tatatgtttt ctttcaaagt aagttttatg ccggcatgac actccaattt tattaccttg
     7981 cagtgagtgt ttacggatgg attacgtgga aaagaggaaa aacggacacc caacccgaat
     8041 tgccggtaac acgaacttcc aaacacatgg ctttcaattt gctgacgggt tttttggcta
     8101 ttttggtgat ttattttttc attttaagta attttaccga ttcgccactt cccagaggtg
     8161 atgcttttac cacagccttg agtatagtgg ctacgtggat gcttgcaaaa aaattgctcg
     8221 agaattggtt gctgtggatt gttgccgatg ccgtttcgac aggtttgtat ctttatcgcg
     8281 gactttatcc tactgccata ctttttactg tttatacgct gatggctgtg ttgggctatt
     8341 ttcagtggaa aaagtccatg aaacatcaga cacattaaaa tgtatttgaa gtaaatgaag
     8401 aatatgctga gagttgctat tttgggtcct gaatctaccg gaaaaaccga gcttgccaaa
     8461 tcccttgccg aatattttca ggtggattgg gtacctgaat ttgctcgcgc gtacgtggaa
     8521 agtttatccc gaccgtacga atatgaagat gtttgtcgca ttgccgccca tcagattgat
     8581 gaagaactgt tttatgaaaa tcatccgagc gatgggaaaa aatttgtttt tttcgatacc
     8641 gatttaatta tcactaaagt atggtttgac tataagtatg gcaaagtacc caaattttta
     8701 caggaaagat taaattgcca tttttttgat ttttatttgt tgtgttatcc cgatttgccg
     8761 tggcaacccg attcggttcg tgaacatgga gacgacagag aattcttttt caactggtat
     8821 aagcaagaaa taaaaaaaac aggaaaatct gtttctatta tcaccggaat cggcaatgaa
     8881 aggctccaaa atgccattcg ggctttgact tcttttgtga gatatttata aattttatta
     8941 ttcaaattta tggaaaagga aattttagat gccatcatcg aattatcgaa aatagatcag
     9001 tataaatttg catgtcatca gcatgttaga aatgagcgaa tgccttccat caataatttg
     9061 caggaaattg ttcagttgtg ccgttctgtt ttgtttccgg gctattttga cgatgcagcg
     9121 ctcgattccg atacgttgct ttatcatatt gggcttagta tcgatcgatt gaaacttttg
     9181 cttaccgagc aattgaaagc gggattctgt ttcgaaagag aacattccac agataaaaat
     9241 ttatgcagcg acatggagca gaacgttgtt caaaaagtga atgctttcat agctcaattg
     9301 tctgaaatcc gcagaaagct ttataccgat gttattgccg cttacaatgg cgaccctgct
     9361 gccaaaagta ttggagaagt gattttttgt tatccgggaa ttcgagccat cagcaattat
     9421 cgtattgctc atgcgctact caaactcgat gtccctttga ttccacgtat tatcagtgag
     9481 ctggctcatt cggaaaccgg tatcgatatt catcccggag ccgaaattgg agattatttt
     9541 atgatagatc atggcacagg tgtggttatt ggagaaactt cggtgattgg aaacaacgtg
     9601 aaaatgtatc aaggcgttac gctgggagcc agaagttttc cgttcgacga gaatggaaat
     9661 cccataaaag gtatcgttcg tcatccacga attggcaaca atgtaatcat ttattccaat
     9721 tctaccattt tgggaaatat caccgttggt gatggtgccg taattggcgg taatctttgg
     9781 gtggatagcg atgtcccggc cggggcaaag ctttcgcagg acgataaact gaaacgttga
     9841 ttgttaaccg atgaaaataa cttccggaaa cgacaataaa atcaggaaat tctttgccga
     9901 gaaggagttt cctatgatag ccaagacctt tcagggactc gaagaggtat tggctgaaga
     9961 acttaccgaa ttgggagcca acaatgtaca aatagagcgg cgcgcagtga gctttagcgg
    10021 tgataaggca atgttgtaca aagcaaattt gcattgcaga accgccttac gtattcttaa
    10081 accgattttt tcttttgaag ccgacaatac agacgaatta tataaaaaaa taaagtcggt
    10141 ggaatgggac gctcttctgc atgtgaataa tactttttcg attgatacaa ccgttttttc
    10201 tgatgaattt cagcattcgc gttttgtaac ctatcgggtg aaagatgcca tagttgactg
    10261 gttttcagag aattttaacg atcggccaag tgtttcgatt gaagaccccg attttgtgtt
    10321 taatattcac atcgccggaa gtcattgtac tttgtcgctc gacagctccg gtgaatcgct
    10381 tcataaacgg ggttatcgaa ccggacagac aaaagctccg ctgaatgaag ttttggcggc
    10441 cggaatgttg atgttggccg gttggaaagg acaatccgat tttcttgatc cgatgtgcgg
    10501 ctccggaact ttgcttatcg aggcagcctt gattgccttg aatatccctc ccggtatttt
    10561 tagaagttct tttgcttttg aaaaatggcc cgatttcgat aaggtgcttt ttgatgattt
    10621 atataatgat gattcacagg agctactttt tgaacatcat attttcggat cggacatctc
    10681 agtatctgcc attaaaattg cccagcagaa tgttaaaagt agcggactgt caaaatatat
    10741 ttctttaaag gtactgccgt tggaaaagct tgaaccgcct tcatccgatt gttttattgt
    10801 taccaatcca ccttatggcg aaaggctccc atcaaaagac atttttgaat tatatgccaa
    10861 cttgggatca ttgctgaaac gtaaattttc gggttgtact gcttgggtga tcagttccaa
    10921 tgaggaagca ctgaaaaaaa ttggtttaaa accttcaaaa cgttatcggt taatgaacgg
    10981 acctttggaa tgctggtttc agcagtatga actttttacg gggaaaagaa acgattattt
    11041 gcagaaaaag aattcctgat tccagttgta tttataatct tttgaatatt agttattaaa
    11101 gtttcttttt tgactgaaat tattaaattt cggcattttt gcttttggga atcaaaaaaa
    11161 taatttactt ttgtacccgc taatgcccag atggcggaat cggtagacgc gctggtctca
    11221 aacaccagtg gattcacttc catgccggtt cgaccccggc tctgggtact ttaatc
//
LOCUS       DAOSIB010000068        11230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_5733_length_11230_cov_10.856644, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000068 DAOSIB010000000
VERSION     DAOSIB010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11230
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5733_length_11230_cov_10.856644"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLD44_06720"
     CDS             complement(<1..224)
                     /locus_tag="PLD44_06720"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOF99062.1"
                     /translation="MENILAVVAHPDDLEIMAGGTVLKYKSLGAKIHVLVITNGSWIS
                     EKGETMRSINEAKLEFENVSKFMQYDSYEM"
     gene            complement(228..1088)
                     /locus_tag="PLD44_06725"
     CDS             complement(228..1088)
                     /locus_tag="PLD44_06725"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOF99063.1"
                     /translation="MKTIIISGSSGFIGKNLINLISQTNKYQIITLDINNGIDLTQNE
                     QLLSLPSFEIFIHLANLLYVPASYQNPEVFYRVNYLTTLNALELCRKNNAKMIYISSY
                     LYGNPVYLPIDENHPINPTNPYAQSKYICEKLCEGYYRDFKIKIIILRPFNIYGIGQK
                     GDLLIPEIIKQLKQGKNKISLKDPYPRRDYINVIDVARAIIKAVDKNVDYTIYNVCSG
                     ISYSVKEITDIINSKLKKPVEFIFSNSDRQYEINETRGSYSKIEKELNWYPLIKFEEG
                     IEAILNKENL"
     gene            complement(1220..2194)
                     /locus_tag="PLD44_06730"
     CDS             complement(1220..2194)
                     /locus_tag="PLD44_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent 4,6-dehydratase LegB"
                     /protein_id="HOF99064.1"
                     /translation="MKILVTGADGFIGSHLTEMLLEKGYRVKALAYYNSFNDWGWLEG
                     IRHPHLEVVTGDIRDPHLCKHITKDIDIIFHLAALIAIPYSYIAPDSYVDTNIKGTLN
                     ICQAARENGVGRVIVTSTSEVYGTAQYVPIDEKHPKQPQSPYSATKIGADALAMSFFN
                     AFELPVIIARPFNAYGPRQSARAIIPTIITQIANGKKEIQVGDLTPTRDFNYVKDTCK
                     GFIELAACDEAIGQEVNIAGNQEISMRDTLQLIAKIMNADIAFVEDKQRIRPKNSEVF
                     RLRGDNTKIKQLTGFTPSYSLEDGLRETVKWFLDKNNLKKYKYEIYNV"
     gene            2905..3585
                     /locus_tag="PLD44_06735"
     CDS             2905..3585
                     /locus_tag="PLD44_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOF99065.1"
                     /translation="MKKQVSIFFMLCGLLFTVCLITANIVEQKLIALGPIEATAGLLI
                     FPVSYIVNDLIAEVWGYRKARLIIWSGFAMNFLAVVIFRLSMLIPPSHNFQNQEAFAL
                     IMGNTLRITIASFVAFLVGSFLNAFVMSKMKILQHGRHFSVRAIVSTLVGEGCDSLVF
                     FSLAFAGVIPTRDLFLLMLTQTAMKTGYEIVVLPLTNFVVNWMKKKEETDVFDYDISY
                     SPIKLKEI"
     gene            3694..4830
                     /gene="nspC"
                     /locus_tag="PLD44_06740"
     CDS             3694..4830
                     /gene="nspC"
                     /locus_tag="PLD44_06740"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HOF99066.1"
                     /translation="MIDFSKIPSPCFVLDEHALRRNLELIRYVKEESGAEIILAFKAF
                     AMWSVFPIIKEYGITKSAASSLAEARLAYEEMGNKAHTYSPVFSQKEFSEILSYSSHI
                     TFNSLLQFHQFHSQAKAKGVSCGLRINPEYSEVKTDLYNPCSPGSRLGVTFDLLGEKL
                     PDGIEGLHFHNLCESNSYELEKTLEEVKKKFGKYFSQVQWINMGGGHLMTKEGYDIEH
                     LISIIRSFKKSYSHLKVILEPGSAFAWQTGVLLATVMDIVENHGIKTAMLDASFACHM
                     PDCLEMPYKPAIIGATDEQKGKPTYRMGGNSCLAGDFFGNWSFENELKIGDKIIFEDM
                     MHYTMVKTTMFNGVTHPSIGIWTKENKFKLIKKFGYEDYKSRLS"
     gene            complement(5725..5800)
                     /locus_tag="PLD44_06745"
     tRNA            complement(5725..5800)
                     /locus_tag="PLD44_06745"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5762..5764),aa:Pro,seq:ggg)
     gene            complement(5919..9182)
                     /gene="carB"
                     /locus_tag="PLD44_06750"
     CDS             complement(5919..9182)
                     /gene="carB"
                     /locus_tag="PLD44_06750"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HOF99067.1"
                     /translation="MEKKQVAKHQYQSPKKGEIKKVLVLGSGALKIGEAGEFDYSGSQ
                     ALKALKEENIQTVLINPNIATVQTSEGIADKVYFLPVTPYFVEKVIEKERPDGIFLSF
                     GGQTALNCGVALYQQGVFEKYNVTVLGTPVQSIIDTEDRQLFNQKLSEIEVKYIKSHA
                     VTNVNDAVSAAHDLGYPVIVRAAYALGGLGSGFCNNDEELHALVEKAFSYSPQVLVEK
                     SLKGWKEVEYEVVRDRYDNCITVCNMENFDPLGIHTGESIVVAPSQTLTNSEYHKLRE
                     LSIRIIRHIGIIGECNIQYALDPFSEDYRVIEVNARLSRSSALASKATGYPLAFIATK
                     LGLGYGLPELKNSVTKTTSAFFEPALDYIVCKIPRWDLSKFNGVSHQLGSSMKSVGEV
                     MAIGRCFEEVFQKGLRMIGLGMHGFVANREYFSDSLETALSQPTDKRVFYLAQALHEG
                     YSIDKLYELTKIDRWFLFKLQNIVRLEHELQEYDSLEKLPNELLLEAKKAGFSDFQVT
                     RLITKCLPEEIEEKMKLTRVHRQRHNILPSVKQIDTLAAEYPALTNYLYLTYNGCGND
                     VQHEDDQRSVIVLGSGAYRIGSSVEFDWCGVNALTTIRKEGYRSVMINYNPETVSTDY
                     DMCDRLYFDELTFERVMDIIEMEMSKGVVVSTGGQIPNNLAMKLANAKVNILGTQAEF
                     IDMAEDRHKFSAMLDQLGIDQPRWKELTTFEEVNEFVERIGYPVLVRPSYVLSGAAMN
                     VCYDASQLEEFLKLATNVSKQHPVVISEFLERCKEIEIDAVADKGEIIVYAISEHIEF
                     AGVHSGDATTQFPPQKIYVETIRRIKNIARQIARSLNISGPFNIQFLARDNYIKVIEC
                     NLRSSRSFPFVSKVLKINFIEIATKIMLGIPVEKPEKSAFDLDYVGVKASQFSFARLQ
                     QADPILGVDMASTGEVGCLGDDSSEALLKSLLSVGITIPKKNILISSGEPKSKVDLLD
                     ACRLLYRKGYQIYATRGTHQFLMDNGVASIPVQWPDEEGEYNVKDMIAAKKFDLVINI
                     PKNVTRRELANDYTIRRSAIDFNIPLLTNARLASAFIFAFCNLTMEDVKIKSWDEYH"
     gene            complement(9225..10289)
                     /gene="carA"
                     /locus_tag="PLD44_06755"
                     /pseudo
     CDS             complement(9225..10289)
                     /gene="carA"
                     /locus_tag="PLD44_06755"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
     gene            10722..>11230
                     /locus_tag="PLD44_06760"
     CDS             10722..>11230
                     /locus_tag="PLD44_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99068.1"
                     /translation="MKRKIGCLLIVLCILSNINAQDNFFPYASAVWIENNGNGQFYNI
                     YSISTTTSGRTYTAQENAIEQGGGNWQSALFQGRDFGSHAANSHTLLFRGAEVKTYKK
                     NSGNVCSAKVFYVVYPQGQRPSSPSFTEHNIAWFDNCDLNIYLFPDDPGHGPCNESGY
                     QKWQSWASGG"
BASE COUNT         3302 a         2129 c         2076 g         3723 t
ORIGIN      
        1 aacatctcat aagaatcata ttgcataaat ttagatacat tttcaaattc cagtttagct
       61 tcattgattg atctcatggt ttcccccttt tcagaaatcc acgatccatt tgtaataact
      121 aatacatgta tttttgctcc aagactctta tattttaata ctgttcctcc agccatgatc
      181 tctaaatcat ctggatgagc gactactgct aaaatatttt ccattatcta taaattttct
      241 ttattaagta ttgcttcgat tccctcttca aattttatta atggatacca attaagttct
      301 ttttcaattt ttgaatatga tcctcttgtt tcattaattt cgtattgtct atctgaatta
      361 ctaaaaatga attctacagg tttttttaat ttagaattaa taatatcagt tatttctttt
      421 acactatagc ttatcccact acaaacatta tatatagtat aatctacatt tttatcaacg
      481 gctttgatta ttgctcgtgc aacatcaatc acgtttatat agtctcttct aggatatgga
      541 tcttttaaag atattttatt cttaccttgt ttaagctgtt tgataatttc gggtattaat
      601 aaatctcctt tttgaccaat tccataaata ttaaaaggtc ttaaaattat tatttttatt
      661 ttaaaatccc tataataacc ttcacaaagt ttttcacata tatattttga ttgagcataa
      721 ggattagtag gatttattgg atgattttca tcaataggta aatatacagg attgccatat
      781 aaatatgaac tgatataaat cattttagca ttattttttc tacacaattc taatgcattt
      841 aaagttgtca aatagttaac tctgtaaaac acctcaggat tttgataaga agcagggaca
      901 tataataaat tggccagatg tataaagatt tcgaaagaag gtagacttag taattgttca
      961 ttttgtgtta aatctattcc attattaata tctaatgtga taatttgata tttatttgtc
     1021 tgtgaaatta gatttattaa attcttacca ataaaaccag aacttcctga aataataata
     1081 gttttcatga ataaccttgc tttaaaacat taaaatgtac gtgaaatttt tgtagagttc
     1141 gtttccttta aatgtcggtg catgtaaagg aacggtttca tgtgtatcgt ataagttatg
     1201 tataaaagct ataatatctt catacattgt atatctcgta tttatatttt ttcagattat
     1261 ttttatcgag aaaccacttc accgtttccc gcaagccgtc ttcgagagaa taagagggtg
     1321 taaagccggt taattgtttt attttggtgt tgtcgccacg taaacggaat acctcggagt
     1381 ttttggggcg aatccgttgc ttgtcttcca caaaggcaat atcggcgttc ataattttag
     1441 caatcaattg caacgtgtcg cgcatagaaa tttcctgatt gccggcaata ttcacttcct
     1501 gaccgatggc ttcatcgcac gctgccaatt ctataaatcc cttacacgta tctttcacgt
     1561 aattaaaatc gcgagtaggc gtaagatctc ccacttgaat ttcttttttc ccgtttgcta
     1621 tttgcgtaat aatagtaggg atgatggctc ttgccgattg acggggaccg taggcattaa
     1681 acggtcgggc aataatcacc ggaagttcga aggcattgaa aaaactcatg gccaaggcat
     1741 ctgcaccaat ttttgtagcc gaataaggcg attgaggttg ttttgggtgc ttttcgtcga
     1801 tcggcacata ttgagcagta ccgtaaactt ccgatgttga ggttacgatt accctgccga
     1861 caccattttc tctggcagcc tggcaaatat taagcgttcc cttgatattg gtgtccacgt
     1921 aactgtctgg tgcaatgtag gaataaggaa tagctataag cgctgcaaga tggaagataa
     1981 tgtcaatatc tttcgtgatg tgtttgcaca aatgcggatc cctaatgtct cccgtcacca
     2041 cttccaaatg agggtgacga ataccctcca accaacccca atcattgaac gaattataat
     2101 aagccagtgc ttttacccga taaccttttt ccaacagcat ttcggtaaga tgtgatccga
     2161 taaagccatc tgctcctgtt accaatatct tcattcgtta aaattcttaa ttttattcaa
     2221 acaaactttc acttaaattt ttctttgcaa aataactact ttttgctaaa acagcaaaat
     2281 atattttcgc tttgtttgaa tttttttcaa aaatggtatc taactaacaa tataaaattc
     2341 cgaataattt attgtcaatg aatgaattag tagagacgca gcatgctacg tctctactaa
     2401 acaggttgaa ttacaattgt ttatttataa acacatgttt ttggttttaa tgaatatcat
     2461 tgtttatggc cctgtcggat ttgcaatccg gcaatttgtt acctgtggat ttgaaatctg
     2521 ctgttactcc aacaaaaaat gacaaatcct ttgcgacaat gtgttattgt ttgttagacc
     2581 ttattttgct ggttaacctt agtaacaagg caaaacttcc ttcccatcta aaaccttatt
     2641 agcttatttt taactgcgaa atagcctgga ggcaacgccg aacattattt tttattttta
     2701 taactcgttg attatgaatt aatttgtaga tatgtagcgt gctgcgtatc tactgaacaa
     2761 tttgaatatt gtatttttaa aaaagtacac ttttggggac tacctgcttt tgttttttaa
     2821 agaaataagc gttaattttg caaaaagttt ctgctgcttt tcttgcagaa cgtgtgcttg
     2881 aacaccacgt aaaaaacaag acatatgaaa aaacaagttt ccattttctt tatgttgtgc
     2941 ggtttgctgt ttaccgtctg tcttattacc gctaacattg ttgaacaaaa actgattgcc
     3001 ttaggcccta tcgaagccac tgccggtttg cttattttcc ctgtttccta tattgtgaac
     3061 gatttgattg ccgaagtttg gggctatcgg aaagcacgtt tgattatttg gagcggattt
     3121 gccatgaatt ttttggctgt ggtcattttc aggttaagca tgcttattcc accttcacat
     3181 aattttcaaa atcaggaagc ttttgctttg attatgggaa atacgctacg cattacgatt
     3241 gcgagctttg ttgcttttct cgttggttct tttctgaatg cttttgttat gagcaagatg
     3301 aaaattttac aacatggtcg tcatttctct gttcgagcta tcgtttccac gcttgtggga
     3361 gaaggatgcg actcgctggt gtttttttcg ctggcatttg ccggtgtcat cccaacccgc
     3421 gacttgtttc tcttaatgct tactcagacg gctatgaaaa cgggatacga aattgttgtt
     3481 ttgcccctta ctaattttgt ggtcaattgg atgaagaaaa aggaggaaac agacgtattt
     3541 gactatgata tttcttacag tccgattaag ctgaaagaaa tataaaaata aaattgtgta
     3601 gaagttgttc tcaaaccatc tttgcatcaa actgtatcta tgtaaataga aaataatcct
     3661 gtaactttgt aaaaaaatta acgatcacac tgcatgatag atttttcaaa aattccttct
     3721 ccctgctttg ttttggatga gcatgcactt cgacgaaatc ttgaattaat acgatatgtg
     3781 aaggaagaat cgggagcgga aataatactg gcttttaagg cttttgctat gtggagcgtg
     3841 ttccctatta ttaaggaata cggtattact aaatcggctg ccagttctct ggcagaagcc
     3901 agattggcat acgaagaaat gggaaataag gctcatactt attctccagt tttttcccaa
     3961 aaagaatttt ccgaaatttt atcttacagt agccatataa cttttaattc cctgctacag
     4021 tttcatcaat ttcattctca ggctaaagca aaaggtgtgt cttgtgggtt acgaataaat
     4081 ccggagtatt ccgaagtaaa aaccgattta tacaatcctt gttcgccagg gtcaagactc
     4141 ggagtaactt tcgatttact gggggaaaaa ctaccggatg gaatagaagg cttgcatttt
     4201 cacaacttat gtgaatctaa ttcctatgaa ttggagaaaa cgctcgaaga agtaaagaaa
     4261 aaatttggca aatatttttc gcaggtacaa tggatcaata tgggtggtgg acatttgatg
     4321 acaaaagaag gttacgatat cgaacatttg atttcaatta ttcgttcctt caaaaaatcg
     4381 tattcccatt tgaaagtgat tcttgagccc ggaagtgcct ttgcctggca aacaggagtt
     4441 ctccttgcca cagtaatgga tattgtcgaa aatcacggaa taaaaaccgc aatgctggat
     4501 gcttcttttg cctgtcacat gcccgattgt ttggaaatgc cttataaacc tgcaattatt
     4561 ggagccacag atgagcaaaa ggggaaacct acctatcgta tgggagggaa cagttgtctg
     4621 gcaggtgatt tttttggaaa ttggtcattc gagaacgaat tgaaaattgg cgacaaaatt
     4681 attttcgaag atatgatgca ttatacaatg gtgaaaacca ctatgtttaa tggagttaca
     4741 catcccagca tcgggatatg gacgaaagaa aataaattta agcttataaa aaaatttgga
     4801 tatgaggact ataagagtcg gttgtcgtaa aatatgattg cctaagtttc ttaccatatc
     4861 agtatctgcc aaaatatatt ctttgtctta gtacatgaca aaaaaatttt taaaaacatt
     4921 tatatttttt attttcagat atttaataaa ggggctttgt tccaaacttc acttactttt
     4981 ttgtcttgaa acaaaaaagt aagaaaaaaa attaacttag ttgaagtagc tacgctcaac
     5041 ataaatcaaa taaatttgat tttgttttcg cttaaacgtt cctttcaaca ctgtgcccac
     5101 ttcactcaaa aaacttacgt tcgaaaagct gaaatcgttc aaactcattc caacgaaata
     5161 cgtcagaact ggtactgaag tattatatgg aaattttcaa aaatcttttt gaatattttt
     5221 ttaccctttt ttatctcaat gtctttaaaa actaattctc tttgagaaac agaggggggc
     5281 tcttcaatta ttttttcaac aaaaggttta tcaattttca gtttaaaaat atatagatac
     5341 ccaatttata aggagttaat caatagttgt tcaaaaaaat ttttattttt aatattcccc
     5401 cttaattctt ttttcgatcc aattttctgc ctgtatgttc acatcgaacg gaattttaaa
     5461 aaactaaccg tgcgtaacgg aaaatttcaa aggataagaa cccttttttt tatactcctt
     5521 ttgtcgaggt attatttttc agaagccaaa agtgtgttta taaatttgca gtagatttgc
     5581 agtaaaattg gtcaaaaatc ggagaaaact tttagaattt gggaaaaagt ccaacttgtt
     5641 tttacaataa tattgaatta aaaatacaaa aaagcctttg aaacaaatat attactattt
     5701 gattatcaaa gactttatat tttgtcggga tgactggatt cgaaccagcg accacacgcc
     5761 ccccagacgc gtactctaaa ccggactgag ctacatcccg aatggttgaa aacggaatac
     5821 aaaattaagc aattttccct gtatttgaaa aatgtttttc tgcttatttt gaactgttct
     5881 tttccctatt tccttaattg cttgaaaatg aaaatatttt aatgatattc atcccaactt
     5941 tttattttaa catcttccat tgtcaggttg cagaaagcaa aaataaaggc acttgccagc
     6001 cgtgcattgg tgagcagagg aatattaaaa tcaatggcac ttcgccgaat ggtgtaatcg
     6061 ttagccaatt ctctgcgcgt aacatttttt ggaatattga taaccaaatc gaattttttg
     6121 gctgcaatca tgtcttttac attgtattca ccttcttcgt cgggccactg aacagggata
     6181 ctcgccacgc cattgtccat caaaaactga tgagttcccc gtgtggcata aatttggtat
     6241 cctttcctgt aaagcaatcg acaagcatcc aacaaatcta ctttcgactt gggttcgcct
     6301 gaagaaatca aaatgttttt cttcggaatg gttattccaa ccgacagtaa ggatttcagg
     6361 agagcttccg atgaatcgtc acccaaacag ccaacttcac cggtagaagc catatcgact
     6421 cccaaaattg ggtcggcttg ctgcaagcgt gcaaaggaga actgcgatgc ctttacccct
     6481 acataatcga gatcgaaagc cgatttttcg ggtttttcta ccggaatacc caacatgatt
     6541 tttgtggcaa tttctataaa attgattttc agtactttcg atacaaacgg aaagctgcgc
     6601 gatgagcgca aattgcattc tatcactttt atatagttgt cgcgagccag gaactgaata
     6661 ttgaatggac ccgaaatatt gagtgagcga gctatctgac gggcaatatt tttaattcgg
     6721 cgaatggttt ccacataaat tttttgcgga ggaaactgtg ttgtagcatc tcctgaatga
     6781 acacctgcaa attcgatatg ttccgatatg gcataaacaa taatttctcc tttgtcggca
     6841 acagcatcta tttctatttc cttgcaacgt tccaaaaatt ccgaaataac taccggatgc
     6901 tgcttggaaa cattggtagc cagttttaaa aattcttcca attgcgaagc atcgtaacaa
     6961 acgttcatgg cagcgcccga aagtacatac gaaggtcgaa ccaaaacggg gtaaccaatt
     7021 cgttcgacaa attcgtttac ttcctcaaaa gtggtcaact ctttccagcg tggctggtcg
     7081 ataccgagct ggtcgagcat ggctgaaaat ttgtgtctgt cttctgccat gtcgataaat
     7141 tcggcttgcg taccaagaat attgactttt gcattggcaa gcttcatggc cagattattg
     7201 ggaatttgtc cacctgtgga caccacaacg cctttcgaca tttccatttc gataatgtcc
     7261 atgacacgtt caaaggtcag ttcatcgaaa taaagccggt cgcacatgtc ataatcggtg
     7321 gaaaccgttt cgggattata attaatcatt accgagcgat aaccttcctt ccgaatggtg
     7381 gtcagtgcat tcacgccaca ccagtcgaat tcgacggaag aaccgattcg gtaagctccc
     7441 gaccccaata caataaccga acgttgatcg tcttcgtgct gaacatcgtt gccgcagccg
     7501 ttgtaggtta ggtaaagata attcgtcaaa gccggatatt cggctgccag cgtgtcaatt
     7561 tgcttcaccg atggtaaaat attgtgtctc tgtcgatgaa cgcgggtgag tttcattttt
     7621 tcctcaattt cttccggcag acatttggtt atcaaacgtg taacctgaaa atcggaaaag
     7681 cctgcttttt ttgcttccaa cagtaattca ttcggaagtt tttcgagtga atcatattcc
     7741 tgaagttcat gttcaagccg gacgatattt tgtagcttaa aaaggaacca gcgatctatt
     7801 tttgttaatt cgtacagttt atcgatggaa taaccttcat gtagcgcttg agccagataa
     7861 aatacacgtt tgtcggtagg ctgggagaga gccgtttcaa gactgtcgct gaaatattct
     7921 ctgttggcaa caaaaccgtg catacccaat ccaatcatgc gcaatccttt ctgaaagact
     7981 tcttcaaaac aacgaccaat ggccatcact tcaccaaccg atttcatact gctgcccagt
     8041 tgatgggata caccgttaaa tttgcttaaa tcccaacgag gaattttaca tacgatgtag
     8101 tcgagtgcag gttcaaaaaa tgcggaggtt gttttggtaa ccgaattttt taattccgga
     8161 agtccgtaac ccaagccaag tttagtagca ataaatgcca gcggataacc tgtagctttc
     8221 gaagcaagcg ctgaagaccg acttaagcgg gcattgactt caatcacccg ataatcttcc
     8281 gaaaacggat ccagcgcata ctgaatgttg cattcgccta tgataccaat gtgacggatg
     8341 atgcggatgg aaagttcgcg cagtttgtga tattcgctgt tggtaagcgt ttgagaagga
     8401 gctactacaa tactttctcc ggtgtgaatg cccagcggat cgaaattttc catgttgcac
     8461 accgtaatgc agttgtcgta acggtctctt actacttcgt attcgacttc tttccaacct
     8521 ttcagcgatt tttctaccaa aacctgaggt gaataggaaa aagctttttc taccaatgca
     8581 tgcagttctt catcgttgtt gcaaaaaccg cttcccaaac cacccagggc ataagctgcc
     8641 cgtacaatta ccggataacc caaatcgtga gctgcggata ctgcatcatt aacattggta
     8701 actgcgtggc ttttgatata ttttacttca atttccgaaa gtttttgatt gaaaagttga
     8761 cgatcttcgg tatcaataat cgattgaacc ggcgtaccca gcactgttac attgtatttt
     8821 tcaaaaacac cctgctgata aagtgccacg ccgcagttga gcgccgtctg tccgccaaag
     8881 gaaagaaaaa ttccatccgg tcgttccttt tcgataactt tttctacaaa gtagggagta
     8941 accggtaaaa aatatacttt atcggcaatt ccttctgaag tttgaacggt agcaatgttg
     9001 ggattgatga gcaccgtttg aatgttttcc tcttttaatg ctttcaaagc ttgtgaaccg
     9061 gaatagtcga attcgccggc ttcaccaatt ttcagtgctc ccgaacccaa aaccagcact
     9121 ttttttattt cgcctttttt tggactttgg tattgatgct tggcaacctg ttttttctcc
     9181 aattcttctt ctatcagttg aggaatggat ttattttttt cggtcagggt acgtatgagt
     9241 acatcaaaaa gaaattccgt atcggttggt cctgaagagg cttcgggatg aaattgacag
     9301 gaaaaccaag gctttgtttt atgccgtatt ccttcgtttg ttccatcatt catgtttaca
     9361 aacaacggtt cccattcatc gcttaaggtt gaattgtcaa ctgcaaaacc gtgattctgg
     9421 gaggtgataa atgctctttg tgttcctgcc agttgaaccg gttggttatg gcttctgtgt
     9481 ccgtatttca gtttgtatat tttggcgcct ccggccattg agagcagttg gtttcccatg
     9541 caaatcccga atattggctt gtcggtctgc atggcctttc ggatattgtt taccgttgca
     9601 tcacaaaatt ccgggttccc cggtccattg gaaataaaca atgcatcata atccaattga
     9661 tgaaaatcat aattccacgg cacacgaata acggtggtgt cgcgtttgag caggcagcga
     9721 attatgttgt gtttcactcc gcaatcgacc agcaaaatac ggtgcttccc gttgccataa
     9781 gttatcactt ctttgcagct gactttggca acctgatttt cggtgtcggg atccacaaaa
     9841 tcggtatctt caccgggaaa aactattttt cccaacatag ttcctttttc acgtaccagt
     9901 ttggtcaatt cgcgggtatc cacaccaaaa attccgggaa cttcattttc tatcagccaa
     9961 tcgctgaggc tttttccggc attccaatga ctgtaatgaa aagaaaaatc ggaaataatt
    10021 aaagctgatg cctgaatttt ttccgattcg taaaaggtgg ataatccgtt ttgttttttg
    10081 tcgatcggga caccgtaatt acctatgagt gggtaagtga gtgtcagtaa ctgacccttg
    10141 taggatgggt cggtcaaact ttcgggatag ccaaccatag cggtattgaa aactacttcg
    10201 cctgcggccg atttttcgta accgaaagat tttccctcga agatggttcc atcttccaaa
    10261 ataaggtgga caggtttgaa ttgttgcata aattccttat aaacttttga aaagtagcag
    10321 gcaaaactat aaaaaaatcc gcctgaaaaa atggtacatc caaatttttg caaagatact
    10381 tatttttctt ggttggagca aaaattggca gaggaaaaat tggaaatctt gatgaggaag
    10441 gaacggtgtc aaattaacaa taattcaagt tgtttgcaaa cgtttgcaac gatttttttc
    10501 gatttttatt gcattttgtt tgcatatagg aaaatcatgg ctttaatttg gaaagtttta
    10561 ccttaaatgt agtatcatga atcagaaatt cttgtcaacc caaaacaagc ctgtttttaa
    10621 cctgacagct catgagtatt ttgcaatgaa aatgttgaag gtaatacttt tgattattac
    10681 taatgatttt tttgttgaac aaatataatt tttattttcg gatgaaaaga aaaattggct
    10741 gtcttttgat tgttctttgc attttatcca acataaatgc tcaggataat ttctttcctt
    10801 atgcctctgc cgtgtggata gagaataatg gtaatggtca attttacaac atttattcca
    10861 tttccaccac tacttctggt cgtacttata ctgctcaaga aaatgctata gaacagggtg
    10921 gtggtaattg gcaatcagca ttatttcaag gtagagattt tggttctcat gcagccaatt
    10981 ctcatacctt acttttcaga ggagctgaag taaaaactta taaaaaaaat tcaggtaatg
    11041 tttgttcagc aaaagttttt tatgtcgtat atcctcaagg tcaacggcca tcatctccat
    11101 cctttactga acataatatt gcgtggtttg ataactgcga tttgaatatt tacttatttc
    11161 cagatgatcc tggtcatggt ccctgcaatg aatccggcta tcagaaatgg caatcgtggg
    11221 cttcaggtgg
//
LOCUS       DAOSIB010000069        11156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_5774_length_11156_cov_12.729574, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000069 DAOSIB010000000
VERSION     DAOSIB010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11156
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5774_length_11156_cov_12.729574"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            356..961
                     /locus_tag="PLD44_06765"
     CDS             356..961
                     /locus_tag="PLD44_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOF99069.1"
                     /translation="MKRRILILFTVLFTVFGQSLFAQMDEPIPPGGARLMVEIKGNDT
                     IFLAYLHDLWVFPKMNFKNKKEEQYYWKMVRDVKRTLPYAKLVAQELSETNKKLMMMP
                     NDKERKKFLAQYEKDIYQKYEPELKKLTLSQGKLLIKLIDRECNNTSYDLIKLYRGSF
                     RAFFYQGFARLFGANLKSEFDYNEKDKILDRIIVLVEAGQL"
     gene            973..1536
                     /locus_tag="PLD44_06770"
     CDS             973..1536
                     /locus_tag="PLD44_06770"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOF99070.1"
                     /translation="MTIFDNTKEQKKEIRNRIRELMRQISDTQKEKQAESVFLKIEIL
                     PAFVQAKNILLYWSLPDELPTHTVVEKWATDKQILLPSVKGENFVIKKYSGLHALKKG
                     AFGIEEPLEIFDWHSPIELVIVPGVAFDKNKNRLGRGKGYYDRFLKSIDALKIGVGFD
                     CQLIEKIPVGKFDVPMNKVVVPSLIID"
     gene            complement(1544..2269)
                     /locus_tag="PLD44_06775"
     CDS             complement(1544..2269)
                     /locus_tag="PLD44_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99071.1"
                     /translation="MPYRRLPNTDQSRLRALRIALEKLENQDFTHQIVNFKTIHEAES
                     ILGVFENQMQQYRQALKNQVEANKRYQKLVGNVRMYISHFIQVLNLSVIRGDIKKEHK
                     KLYQLDPEVNNVPDLTSEAALLHWGKCIIDGENERLRLGGFPIYNPTIAKVKVHYEIF
                     KENKLNQKLYQSNTSRTLEDLGNLRIKVDEIIIDIWNQVEANFKDEKPYSKIQKCKEF
                     GLIYYYRKGEKKLLPEDAEEQKK"
     gene            complement(2318..3286)
                     /locus_tag="PLD44_06780"
     CDS             complement(2318..3286)
                     /locus_tag="PLD44_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOF99072.1"
                     /translation="MIPFSEITKIINTELNKLNWDKKPEGLYRPIAYVLSLGGKRIRP
                     SLVLMACNLFSDKVESAIAPALGIEIFHNFTLLHDDIMDKAELRRGQPTVHKKWDENT
                     AILSGDLMQIAAYQLIGKVDELHLKKVLDIFSETAAEVCEGQQYDMEFEHRENVSPEE
                     YLEMIRLKTAVLLGAALKIGAIVGNANSKDANLLYEFGINLGMAFQLKDDLLDVYGDE
                     QFGKKIGGDIVANKKTYLLISAQKLAKGADAKELEKWLKTDSENETKVEAITALYNRL
                     EVRDICENKMQEFFEKSIDCLNRISVEAAKKIELRHLAEQLMSRKE"
     gene            complement(3294..4289)
                     /locus_tag="PLD44_06785"
     CDS             complement(3294..4289)
                     /locus_tag="PLD44_06785"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOF99073.1"
                     /translation="MKKNIAIVAGGDSSEVVVSLKSAEGLHSFMDKQRFNVWIVTIIG
                     KTWQVECSDNEKYPIDRNDFSFTRKGEKIVFDFAYITIHGTPGENGILQGYFNLIKLP
                     FSCCDVLAAAVTFNKFTCNQFLKNFDVKTAASVLLRKNEEIDLHEISAKTGFPCFVKP
                     NAGGSSFGVSKVKSIDQLEPAIRKAFSEDDEVVVESFLAGTEVTCGMYKTKAKRVVFP
                     LTEVVSQNEFFDFAAKYNGQVQEITPARISGSLTEKVQCQTARIYDILGCKGLVRIDY
                     IISEKEEIFLLEVNTTPGMTATSFIPQQIKAAGLNITDVLTEIIENELNYPTLIK"
     gene            complement(4381..>4746)
                     /locus_tag="PLD44_06790"
     CDS             complement(4381..>4746)
                     /locus_tag="PLD44_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOF99074.1"
                     /translation="HYKVLERLTYVTLVECRLETGRTHQIRVHMKHIGHPLFNDERYG
                     GNEILRGTTSAKYRQFIKNCFEICPRQALHAKTLGFVHPTTGKQMNFDSELPEDMQQL
                     LTKWRNYNTGSAAVEKEDF"
     assembly_gap    4747..4846
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4847..5313)
                     /locus_tag="PLD44_06795"
     CDS             complement(<4847..5313)
                     /locus_tag="PLD44_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOF99075.1"
                     /translation="MLEVTKWEDFSDSAEEPELFEHFRFIADKGQSPIRIDKYLVNCM
                     ANVSRNRIQEAADAGNILVNDKPVKANYKVKPNDVISIVMTYPPSQFEIIPQNIPIDI
                     VYEDDDLMVINKQPGLVVHPGVGNYEGTLLNALAYHFKDNPKFDFNDSRIGLV"
     gene            complement(5340..6179)
                     /locus_tag="PLD44_06800"
     CDS             complement(5340..6179)
                     /locus_tag="PLD44_06800"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOF99076.1"
                     /translation="MDFKKFWKESFAGFVVKNLLLAIAIFLALTWITLFIIDIYTHHG
                     EAEIVPDMKGLYVEEAENILREKGLYPKIIDSVYVRDKKAGIIIEQVPPPKSTLKKNR
                     PVYLIINSTQVQKVPVPEVIDVSSRQAIAMLQSVGLDISKIEYKPSEFKDLVIDIKYN
                     GVSILPGTKIPESSSVVLVVGSGYGEGMFSVPSLKGMGLQEAKQAALSNSFVIGMVNF
                     DIPPKGNDADYFIYRQLPKAGTSMPAGSRIDIWLTSSPTKLLEDKEEDQIIILPENDE
                     VFF"
     gene            complement(6355..6513)
                     /gene="rpmH"
                     /locus_tag="PLD44_06805"
     CDS             complement(6355..6513)
                     /gene="rpmH"
                     /locus_tag="PLD44_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOF99077.1"
                     /translation="MKRTFQPSQRKRRNKHGFRERMTTANGRRVLASRRAKGRKKLTV
                     SDEGRPKL"
     gene            6946..8493
                     /locus_tag="PLD44_06810"
     CDS             6946..8493
                     /locus_tag="PLD44_06810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOF99078.1"
                     /translation="MKKRTIWILIILMVVTIVGLVLVQIRYVRINAEMIENQFKENVQ
                     RSLFQAVRLVEENEALQYLSETLEDVDYSAKSKGLIKSGNAQKDVLGMTTDSMAMDSG
                     KDFTLNQKLPSIQLSTKHGKATIEETSRYLQKKFQQDFSRSKTILDQAVFRWLRESDQ
                     KEISQRVNFDELNDILQTLLENNGVTLPFNYSVVDKQGRLVYKHTNISVDEQKISQTG
                     NVFTQKLFPFEETNQPYYLQVDFPTRQNYILNSMNLLLPSVVLVILILTIFIIAIIII
                     FRQKQLNNIKNDFVNNMTHELKTPIATISLASQMLQDPNIGKTPEMLQHVSKVIHDET
                     QRLSFQVEKVLQMAIFEKEKSPLNLTEIHINSLIQDVIHSFSLRVTNSGGKIIPSLKA
                     EKDLALIDEVHFTNVIFNLMDNALKYSNKPLLLNIETWNEKDRIFISIEDNGIGIRKE
                     DLKRIFEKFYRVSTGNLHNVKGFGLGLAYVKKIITDHKGTIKVESEPNIGTKFTISLP
                     ILKNQEK"
     gene            8490..9200
                     /locus_tag="PLD44_06815"
     CDS             8490..9200
                     /locus_tag="PLD44_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOF99079.1"
                     /translation="MTDEKIKILLCEDDENLGMILREYLQSKGYDVDLLPDGEAGYKA
                     FTHKKYDLCILDVMMPKKDGFTLAADIRALNSEVPIVFLTAKSMKEDILQGFKLGADD
                     YLSKPFSMEELLYRIESIMRRIRGEKAREVTVYQIGNYTFDSQKQLITINGETKKLTT
                     KESELLSLLAANMNNVLERNYALKTIWVDDNYFNARSMDVYITKLRKLLKDDPNVAII
                     NIHGKGYKLIAPAVKEKN"
     gene            9432..10874
                     /locus_tag="PLD44_06820"
     CDS             9432..10874
                     /locus_tag="PLD44_06820"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOF99080.1"
                     /translation="MKLRNFILTVLLFSFLLTYAEIPAGYYNSADGKNASALRTALKT
                     IITNGHSVTSYAGLWTAYKTTDVNANGKIWDMYSNCSFTYGTDQCGSYSNECDCYNRE
                     HTTPQSWFNEAPPMVSDLFNVYPTDGKVNGMRSNYPYGEVGSATYTSGNGSKLGASSF
                     SGYSGTVFEPVDEYKGDFARTYFYMATRYADECGTWTGEATAVYSSSNGLTEYGKNLF
                     LKWSREDPVSQKEIDRNNAVYGIQNNRNPFIDYPGLEEYIWGTKTSEVFYVNASVSKP
                     SVSSPTATNVTYNSAVLGGTVTSTGGGTITETGVYYSTTDGFADGTGTKVSTSSPSTS
                     TFTVSINGLNSSTTYYFKAFATNSAGTGYSSQSSFKTSTLSSGNDPEIILGNPVATGS
                     TLNFGKVTANSTKSLLIKTKNLTGDLSVSVTGEMFNVSTSTISKSNAEEGYLLNVNYQ
                     PTATGSHTGTLTISGGGLTSNYVVQLTGSK"
BASE COUNT         3579 a         2051 c         1966 g         3460 t
ORIGIN      
        1 gccatcataa aaaatgtctt atcaagacag aaaatggcaa ctatcgttat tttaaccttg
       61 agcaggtgcg aacttctgtt ttgagacaaa aactacgaga cattccaaag aaaaacttaa
      121 caatagaaac aatgttgaag cgaccatttt tcaattaggt taccattgta acaaaaataa
      181 cttttatatt gcaaattatt gtaaaatatc aaaacaacaa aaaatttaat gcctctcttc
      241 aaaaaaatct tatttttctc ttttttgaaa aaatcaaatt ttaagaggaa attcaatatt
      301 tgttttttgt ttttttgaaa caatatctga aaaaatatgc gtaactttgt ttgctatgaa
      361 acgccggatt ttgatattgt ttactgtttt atttacggtt tttggtcaat cgttatttgc
      421 acaaatggat gagcccattc cccccggtgg ggcacgttta atggtagaaa ttaagggaaa
      481 tgataccatt tttctggctt atttacatga tctttgggta tttcccaaaa tgaattttaa
      541 aaacaaaaaa gaggaacagt attattggaa aatggttcgt gatgtgaaaa gaacgctgcc
      601 ttatgccaaa ttagtggctc aggaattatc cgaaaccaat aaaaagttga tgatgatgcc
      661 caacgataag gaaagaaaaa aatttttggc tcaatatgaa aaggatattt atcagaaata
      721 cgaaccggaa ttgaagaagt taacattgag tcagggaaag ctattaatta agcttatcga
      781 tagagagtgc aacaacactt cctatgattt gataaaattg tacagaggca gtttcagagc
      841 ttttttctat cagggctttg caagattatt tggtgcaaat ttaaaatcgg agttcgatta
      901 taacgaaaaa gataaaattc tggatagaat tatcgttctg gtggaagccg ggcaactata
      961 aatgaccatt aaatgaccat ttttgacaat acaaaggaac aaaaaaagga aattcggaat
     1021 cggattcggg aattaatgcg gcaaataagc gacacgcaaa aagaaaaaca agccgagtcg
     1081 gtttttttga aaatcgaaat actccctgcc tttgtacagg caaaaaacat acttttatat
     1141 tggtcgctgc ccgatgaact tccaactcat accgttgtag aaaaatgggc tacagacaag
     1201 caaatcttac ttccttcagt aaaaggagaa aatttcgtga taaaaaaata ttccggcctt
     1261 cacgcactga aaaaaggtgc tttcggcatc gaagaaccat tggaaatttt tgattggcat
     1321 tctcccatcg agttggttat tgttcccggt gtggctttcg acaaaaacaa aaatagattg
     1381 ggaagaggaa aagggtatta cgaccgtttt ttgaaaagta tagatgcgct aaaaataggg
     1441 gtcggtttcg attgtcaatt aatagaaaaa attccggtcg gaaaatttga cgtgccaatg
     1501 aataaagttg ttgtaccttc tttgattatc gactaagata cgtttatttt ttttgttctt
     1561 ccgcatcttc tggcaaaagt tttttttctc ccttgcgata atagtaaatc agtccaaatt
     1621 ccttgcattt ttgaattttt gaataaggct tctcgtcctt gaaattggcc tccacctgat
     1681 tccagatgtc tataataatt tcgtccactt ttattcttaa atttcccaaa tcttcgagtg
     1741 tccgcgaagt attgctctga taaagtttct gattgagctt gttttccttg aaaatttcgt
     1801 aatgaacctt aactttggca atggtaggat tataaatggg aaaaccaccc aagcgcaacc
     1861 tctcgttttc accatcaatt atacatttac cccaatgcag caaagcggct tcgctggtca
     1921 aatctggaac attatttact tcaggatcaa gctgataaag ttttttatgt tcctttttaa
     1981 tatcgccacg aattacagaa agatttaaaa cctgaataaa atgagaaata tacattctta
     2041 cattgccaac gagtttttgg taacgtttat tggcttcaac ctgatttttt aatgcttgtc
     2101 ggtattgttg catttgattt tcaaaaacgc ccaaaatgct ttccgcctca tgaattgttt
     2161 taaaattgac aatctgatgc gtaaaatcct gattttcgag cttttctaat gcaattcgta
     2221 aagcccgaag tcgtgattgg tccgtatttg gtaatcttct gtatggcatt tttttcaaaa
     2281 aaaattcttg aaatttatcg atgtcaaaat taaaaaatta ttccttgcga gacattaact
     2341 gctctgccaa atgccttaat tcgattttct ttgctgcttc aaccgaaatc ctattcaaac
     2401 aatcaatgga tttttcaaaa aattcctgca ttttattttc acaaatatct cttacttcaa
     2461 gtcggttgta aagagcggtg atagcttcaa cttttgtctc attttcagaa tcggttttca
     2521 accacttttc caactctttg gcatctgctc cttttgccag tttttgagca gaaatcaaca
     2581 aataggtttt tttgttggct acaatatctc caccaatttt tttcccaaat tgctcatcgc
     2641 cataaacatc gagcaagtca tccttcagct gaaaagccat tcccaagttg attccaaatt
     2701 cataaagcaa attggcatcc ttactgtttg cattgccaac aatagcccca attttcaatg
     2761 cagctccaag taatacggca gttttaagac gaatcatttc cagatattct tcaggcgata
     2821 cattttcacg atgttcaaat tccatgtcgt attgttgccc ttcacaaact tcagcagctg
     2881 tttcggaaaa tatatccagc acctttttta aatgcaattc atcaactttc ccaatcaatt
     2941 ggtaggcagc tatttgcatt aaatctccgg aaagaattgc agtattttca tcccattttt
     3001 tgtgaaccgt cggctgcccg cgtctcagtt ccgctttgtc cataatatcg tcgtgtaaca
     3061 gcgtgaaatt atggaaaatt tcaattccca aagccggagc aatagcactt tccaccttgt
     3121 cggaaaaaag gttgcaggcc atcagaacca gcgaaggacg aattcgcttg cctccaagtg
     3181 aaagcacata ggcaatggga cgataaagtc cttcaggttt tttgtcccaa ttcaacttat
     3241 tcagctctgt attaataatt tttgttattt cactgaaagg aatcattata aatttattta
     3301 attaaagtag ggtaattcaa ttcattttca ataatttctg tcagcacatc cgtaatattc
     3361 aaaccggcgg cttttatctg ttgcggaatg aagctggtgg cagtcattcc gggtgtggtg
     3421 ttgacttcca gaaggaaaat ctcctccttt tcggaaataa tgtaatctat tctgacaagt
     3481 cctttacaac ctaaaatatc gtaaattctg gcagtctggc actgaacttt ttccgttagt
     3541 gagccactga tgcgtgccgg cgtaatttcc tgaacctgac cattatattt ggccgcaaaa
     3601 tcaaagaatt cattctggct taccacttcg gttaacggga aaacgactcg ttttgctttt
     3661 gttttataca ttccgcaagt tacctccgta ccggcaagga aactttctac caccacttca
     3721 tcgtcttcag aaaaagcttt acgaatagcc ggttccaatt ggtcaatact tttcaccttg
     3781 ctaacgccaa aactgctgcc tccagcattg ggcttaacaa aacaaggaaa tcctgttttt
     3841 gcagatattt cgtgcaggtc aatttcttca ttttttcgca acaaaacaga agctgccgtt
     3901 ttaacatcaa aatttttaag aaactgattg caggtaaatt tgttgaaagt tacggcagcc
     3961 gctaacacat cgcaacagga aaaaggcaac ttaatcagat tgaaataacc ctgcaaaatg
     4021 ccattttcac ccggtgttcc gtgtatggta atataggcaa agtcaaatac aattttttct
     4081 ccctttcgag tgaaactgaa gtcgttccgg tcaatcggat atttttcgtt atcagagcac
     4141 tccacttgcc aagttttgcc aataatcgta acaatccata cgttgaatcg ctgtttatcc
     4201 ataaatgaat gcaatccttc agcgcttttt aacgatacca ctacttctga agaatctcca
     4261 cctgcaacaa ttgctatatt ttttttcatc caaaaaaata aaaaattaag tatttatttg
     4321 tcttctttac tgaaaaatca cagttacatt tgcctaatga aaaataaact ttggaaataa
     4381 ctaaaaatct tctttctcca ctgctgccga cccggtatta taatttctcc attttgtgag
     4441 caactgctgc atatcttcag gcagttcgct atcgaaattc atttgttttc cggtagtagg
     4501 atgaacaaat cccaatgttt tggcatgtaa tgcctgacgt ggacaaattt cgaagcaatt
     4561 tttaataaac tgtcgatatt tggcagaagt tgtacctctt aaaatttcat ttccaccata
     4621 acgctcgtca ttaaacaaag gatgaccaat gtgcttcatg tgaacacgaa tctgatgcgt
     4681 acgccctgtt tcgagtcggc attctaccaa tgttacataa gtcaggcgtt ccaacacttt
     4741 ataatgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgaa ccagcccgat
     4861 tcgcgaatcg ttgaaatcga actttggatt atccttaaaa tgataagcca gcgcattcag
     4921 caaagtcccc tcgtaattgc caacacccgg atgaaccact aaacccggct gcttgttgat
     4981 aaccataagg tcatcatctt cataaacaat gtcaatggga atgttttgcg gaataatttc
     5041 aaactgactt ggaggataag tcattacaat ggaaataaca tcattgggtt tcaccttgta
     5101 attggctttc accggcttgt cgttgaccag aatatttcct gcatcggcag cttcctgaat
     5161 ccgattacgt gaaacattgg ccatgcaatt gaccaaatat ttgtcaatcc gaatggggct
     5221 ttgcccttta tccgctataa atcgaaaatg ttcaaaaagt tccggctctt cagccgaatc
     5281 ggaaaaatct tcccattttg ttacttccaa caatttcttt actcgatttt attggttttt
     5341 taaaaaaata cttcgtcgtt ttccggcaaa attattattt ggtcttcttc tttgtcctcc
     5401 aataatttag tgggagaaga ggttaaccaa atatctattc gactgccggc aggcatagaa
     5461 gtccccgctt taggaagctg ccgatatata aaatagtctg catcatttcc tttgggggga
     5521 atatcaaagt ttaccatccc tataacaaaa gaattggaaa gagcggcttg tttggcttcc
     5581 tgcagtccca ttccttttag cgaaggaaca gaaaacatac cctcgccata accacttcca
     5641 acaaccaaaa ctactgaact gctttcaggg atttttgtcc cgggtaaaat actgactccg
     5701 ttatatttta tgtctatgac caaatctttg aactctgatg gcttatattc aattttactt
     5761 atgtcaagtc ctacagattg cagcatggca atagcttgac gagaagaaac atctataact
     5821 tcaggaactg gaactttttg aacttgagtt gaattgatga tcagatatac cggtctattt
     5881 ttcttaagtg ttgatttagg aggtggaact tgttcaataa taattccggc ttttttatct
     5941 ctaacatata cggaatcaat aatttttgga tacaatcctt tttctcttaa aatattttct
     6001 gcttcttcga cgtacaaacc tttcatgtca gggacaattt cagcttcacc atgatgtgta
     6061 taaatatcga ttatgaacaa tgttatccat gtcaaagcta aaaaaattgc aatggccagt
     6121 aaaaggtttt taactacaaa tcctgcaaaa gattctttcc aaaatttttt gaaatccata
     6181 agttatgtta gggtcttttg aacaaagttg aagaagtaaa cttctgtgaa aaaatttatt
     6241 ccaaaagtac agaaataatt ttattatcgg tgtataacaa acaaaaaaag taaaaacctc
     6301 tttcgaagtt cttactttga ataacgttag atgtaatttt tttagtgtat ctgattacaa
     6361 ttttggacgt ccttcatcgg aaactgtcag tttttttctt ccttttgctc tgcgtgaagc
     6421 caaaaccctg cgtccattgg cagtagtcat tctttcacga aatccatgtt tgtttcttct
     6481 ttttctttgc gaaggttgaa atgtcctttt catcgtgttt ggtatttgat gttgttttta
     6541 ctccagattt ttagaccaca aaagtaatgt ttttttttga aatgccaaac atagtttaga
     6601 aaatagatgc tataaaacag aaaactcttt acgataaatt ttttaaagaa aattgagttt
     6661 acagctattg catattgcag tttaaaatcg ttgttatatt tttaacgaac taatagctac
     6721 ttgccggctt tgagaaacga atatcattta aaatttactt aatcattaaa aaaagaatta
     6781 tttttttaaa ctaaaaatgg aatagtctcg ttatgtacta aacatttttc gttagaaatt
     6841 gttttaatta agatatgaat tgtttatgtt aatttaatat tgttaaatct tataaaaaac
     6901 tattttaatt acttcatttt aagtaaattt gcaaaagtat agtgtatgaa aaagagaacg
     6961 atctggatat tgatcatttt aatggtagtt acaattgtgg gtttggtact cgtacaaatc
     7021 cgctatgtac gaataaatgc cgaaatgatc gaaaaccagt tcaaagaaaa cgttcagcga
     7081 agtttatttc aagctgtgcg actggttgaa gaaaacgaag cattgcagta cttgtcggaa
     7141 acattggaag atgtggatta ttcagccaaa agcaagggat taataaaaag tggaaatgca
     7201 caaaaagatg tgctgggaat gacaaccgac agcatggcga tggattcagg taaagacttt
     7261 acattaaacc aaaaattgcc gtcgatacaa ttatcgacga aacatggtaa agcaacaatt
     7321 gaagaaacat cgcgatattt gcaaaaaaaa ttccaacagg atttttcacg ttcaaaaacc
     7381 attttggatc aggctgtttt cagatggctc agagagtcgg atcaaaagga aatttctcaa
     7441 cgggtaaact ttgacgaact gaacgacata ctgcaaaccc tcttagaaaa caacggcgta
     7501 acgctgccgt ttaattattc agtagtggac aaacagggaa gattggttta caagcatacc
     7561 aatatctcag tagatgagca aaaaatatcg caaaccggaa atgtatttac ccaaaaactt
     7621 ttcccgtttg aggaaaccaa ccaaccttac tacctgcaag tagattttcc taccaggcaa
     7681 aattacattt tgaattcgat gaatttatta ctgccatcgg ttgttttggt aatacttatt
     7741 ctgacaattt ttatcattgc gattatcatt attttcaggc aaaaacaatt aaacaacatc
     7801 aagaatgatt ttgtgaacaa catgacacat gaactgaaaa ctcccatcgc tactatttcg
     7861 ttggcttcgc agatgctgca ggatccaaac attggaaaaa cgcccgaaat gctccaacat
     7921 gtatcaaaag taatacatga tgaaacacaa cgattaagtt ttcaggtaga aaaagtactg
     7981 caaatggcca tttttgaaaa agaaaaatcg cctttaaatt tgaccgaaat acatattaat
     8041 tctttgattc aggatgttat acacagtttt tcgttgagag taaccaacag tggtggaaaa
     8101 attattcctt cgttgaaagc cgaaaaagat ttggcactga ttgatgaagt tcatttcaca
     8161 aacgtgattt tcaacttaat ggacaatgcg ctgaaatata gcaacaagcc tctgctccta
     8221 aatatcgaaa catggaacga aaaagacaga atattcatta gcattgaaga caacggaata
     8281 ggtattcgaa aagaagactt aaagcggatt tttgaaaagt tttatcgggt ttccaccgga
     8341 aacctgcaca atgtgaaagg atttggactt ggattggctt atgtaaaaaa aatcataacc
     8401 gatcataaag ggacaattaa agtggaaagc gaacccaata ttggaactaa atttactatt
     8461 tcattaccaa ttttaaaaaa tcaagaaaaa tgacagacga aaaaattaaa attttattgt
     8521 gcgaagatga cgaaaatctt ggcatgattt taagagagta tctgcaatcg aaaggatacg
     8581 atgtagattt gttgcctgac ggcgaagccg gctataaagc ctttacgcat aaaaaatacg
     8641 atttgtgcat tttagacgtc atgatgccaa agaaagacgg atttacactg gcagccgaca
     8701 ttcgtgcgct gaatagcgaa gttcccattg tctttctgac agccaaatcg atgaaagaag
     8761 atattttgca gggtttcaaa ctgggggcag acgactattt gtcgaaacct ttcagtatgg
     8821 aagagttgct gtatcgaatc gaatcgatca tgcgccgtat cagaggcgaa aaggccagag
     8881 aagttaccgt ttatcagatt ggcaattaca cattcgactc tcaaaagcaa ctgattacca
     8941 ttaatggtga gaccaaaaag ctgaccacga aggaatcgga acttttgagc ctgctggctg
     9001 ccaatatgaa caacgtgctg gaacgtaatt acgcattaaa aaccatttgg gtggatgaca
     9061 attacttcaa tgccagaagt atggatgttt atatcaccaa acttcgcaaa ttgttgaaag
     9121 acgatcccaa cgtggccatc attaacattc atggcaaagg ttacaagttg attgctcctg
     9181 ccgtaaaaga aaagaattaa atcattaact ttcagatttt taagaggctg ttccgttttt
     9241 tcagagcagc cttttttatt tttacccgaa aaaattttta aggaaaagta tcaacattca
     9301 aaaatttggc ttaaattagc acccttttct aaaaaaaaaa tcaaatgaaa catgctgaat
     9361 aaaggcttgt tgatttttga tgaatagaaa taacgaaata gcttcaatat tatgaattaa
     9421 aaaatcgact tatgaaattg cgtaatttta tcctgaccgt tctattattt tcatttttgc
     9481 tcacttatgc cgaaattcct gccggttatt acaacagtgc tgacggtaaa aatgcttctg
     9541 cattgcgaac ggctcttaaa acaatcatta ccaacggaca ttctgtaacg agttatgccg
     9601 gattgtggac agcatacaaa actaccgatg tgaatgcaaa cggtaaaatt tgggacatgt
     9661 attccaattg ctcatttact tatggtaccg atcaatgtgg cagttacagc aacgaatgcg
     9721 actgctacaa cagagaacat accacaccac aaagttggtt taacgaagcc cctccgatgg
     9781 tttccgattt gtttaatgtt tatccgactg atggcaaagt gaacggcatg cgaagcaatt
     9841 atccttacgg agaagtaggt tcagcaacgt acacgtcggg caacggaagc aaattaggtg
     9901 caagttcgtt ttccggttac agcgggactg tttttgaacc tgtagacgaa tacaaaggcg
     9961 attttgcacg aacctacttt tacatggcaa cgcgctatgc cgatgaatgc ggaacatgga
    10021 caggtgaagc tactgccgtt tatagttctt ctaatggatt gacggaatat ggaaaaaatc
    10081 ttttcctaaa atggagtcgt gaagatccgg tgagtcagaa agaaatcgac cgaaacaatg
    10141 ctgtgtatgg aattcaaaac aacagaaatc cgtttatcga ttatcccggt ttagaggaat
    10201 atatttgggg aacaaaaact tcggaagttt tttatgtcaa tgcttctgtt tccaagcctt
    10261 ctgtgtcttc accaaccgcc acgaatgtaa cctataacag tgctgttttg ggcggaacag
    10321 ttacaagtac tggaggcgga accattacgg aaacaggagt atattacagc actaccgatg
    10381 gttttgccga tggaacaggt accaaagtat ctacttcttc tccttcaacg agtacgttta
    10441 cagtttccat aaacggatta aactcttcta caacatatta tttcaaagct tttgcaacca
    10501 acagtgcagg aacgggttat tcttctcaat caagttttaa aacttcgacc ttaagttcgg
    10561 gaaacgaccc agaaattatt ctgggaaatc cggttgcaac tggatcgaca ttaaatttcg
    10621 gaaaagttac ggcaaattcc accaaatcat tgttgataaa gaccaaaaat ttgaccggag
    10681 atctttctgt cagcgttacg ggagaaatgt tcaacgtttc taccagcact atctccaagt
    10741 caaatgctga agaaggttat ctgctcaatg tcaattatca gccgacagca accggctctc
    10801 atacgggtac tttaactatt tcaggtggag gattaacttc caattatgtt gttcagctga
    10861 caggttcaaa ataataggat acggtttccc gcaaataaag caaatagcgt tgtcggattc
    10921 aatggcaacg ctttaagtca tgacaaaaat ttaaaaatag aatcccctct taaaatctga
    10981 ttttttcaaa aaagagaaaa aatcagattt ttttgaagag aagtattaaa ttttttgttg
    11041 tttcgatatt ttacaataat ttgcaatata acagttattt ttgttacaat ggtaacctaa
    11101 ttgaaaaatg gtcgcttcaa cattgtttct atggttaagt tcttcttttg gaatgt
//
LOCUS       DAOSIB010000070        11090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_5821_length_11090_cov_13.599456, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000070 DAOSIB010000000
VERSION     DAOSIB010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11090
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5821_length_11090_cov_13.599456"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..590
                     /locus_tag="PLD44_06825"
     CDS             270..590
                     /locus_tag="PLD44_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99081.1"
                     /translation="MEQQQAITYQYDLFDSKGQDVIRSFAGSEAVSGAQAIKALQVKE
                     AGEQKRAITHDLMGGWGSNSPTYSINSALNKIVCKQQINNEKINNLPCEYSLPSVQKD
                     RPYF"
     gene            complement(529..2193)
                     /gene="asnB"
                     /locus_tag="PLD44_06830"
     CDS             complement(529..2193)
                     /gene="asnB"
                     /locus_tag="PLD44_06830"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HOF99082.1"
                     /translation="MCGIVCAFDIKKNSQELRPQVLQMSKRVRHRGPDWSGIFSNDRA
                     ILAHERLSIVDPESGKQPLYSKDGKLVLAVNGEIYNHQEIRKQFDGKYEFLTKSDCEV
                     ILALYREKGPDFLNDLNGIFAFALYDIEKDAFFIGRDHIGIVPLYQGWDKDGTYFVAS
                     ELKALEGYCNRIEEFLPGQYFYSLDGQPKQWYHRDWEDYENVKDNESNIEELRKALEA
                     AVERQLMSDVPYGVLLSGGLDSSVISAIAKKFSPKRIESGNTQDAWWPQLHSFAIGLE
                     GSPDLAAAREVAEHIGTIHHEIHFTVQEGLDALRDVIYHIETYDVTTVRASTPMYLLA
                     RFIKSMGVKMVLSGEGSDEIFGGYLYFHKAPNPEEFHKETVRKIKNLHLYDCLRANKS
                     LASWGVEGRVPFLDKEFLDVAMRLNPKDKMAGNGKIEKWILRKAFENYLPEKIVWRQK
                     EQFSDGVGYNWIDSLKEMTNKLVSDEEMEKAAEKFPIQPPMTKEEYYYRSIYSDLFPS
                     DSAALCVPSVPSIACSTPIALEWDASFKNMADPSGRSVKSIHKEGY"
     gene            complement(2235..3665)
                     /locus_tag="PLD44_06835"
     CDS             complement(2235..3665)
                     /locus_tag="PLD44_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HOF99083.1"
                     /translation="MGNPKAFLYIPRKEAGYRPVQERIYDFGEVEQTLNREDRKLQAS
                     RCMDCGVPFCHWACPLGNKMPEWQDLIYKEKWQEAIDILQETNNFPEFTGRVCPAPCE
                     KSCVLSLHEAPLTIRENEAAITELAFNEGMIKARPPRKRIAKKIAVIGSGPSGLAAAQ
                     QLNRKGYTVTVFEKDAHIGGLLRYGIPNFKLAKNVIDRRLKLMEEEGIIFSPNMEVGK
                     NISAKELLDSFDAICIAVGAGQPRDIQAEGREMRGIYFAMDFLSQQNKQLLGETMQQE
                     EIISAKNKHVLVIGGGDTGSDCVGTSIRQGAVKVTQIEILPQPPIGKNPETPWPYYPL
                     ILKTSSSHEEGCTRRWCLNTKAFIGKNGHVTEVLIEEVEWHREENGQMTMVSTGKTET
                     VKADLVLLALGFLHPVHEGFLNELGVKYDGRGNIEVDKNGKTSVDKIFATGDSVMGAS
                     LVVRAIASGRKAAENIHHFFCHEQKK"
     gene            complement(3705..8219)
                     /gene="gltB"
                     /locus_tag="PLD44_06840"
     CDS             complement(3705..8219)
                     /gene="gltB"
                     /locus_tag="PLD44_06840"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HOF99084.1"
                     /translation="MNYLNLYEQQSLYSFQNEHDACGVGLVVNISGEKSHEIIENGLQ
                     VLENMVHRGAESADNKTGDGAGMLLQIPHEFILLQGIPVPAIGKYGTGLIFLPREKEE
                     SELCLRIIKENVEEENLHLLAVRDMPVNSKILGEISASNEPLIKQIFVTGNLRQEELE
                     RKLYLVRKKIENSIAKSNLSNRRSFYIVSLSSKVLIYKGMLTSLQLRDYYPDLTNPNF
                     TSAIALVHSRFSTNTFPTWDLAQPFRMLAHNGEINTVRGNRLWMEARESVFKSELLGD
                     LKPLFPIVQPAMSDSASLDNVLEFLVMAGKSLPHALAMLVPESWNQKNPISEELRGFY
                     EYHSMFMEPWDGPATLLFSDGRYAGGMLDRNGLRPARYLITNDGMMIIASETGTIEFE
                     PENIKEKGRLKPGKILMVDTEEGKIYYDEELKEQIAKAFPYNEWLERNRINLEEISSG
                     RSVKPEIKNYQNLLIAFGYSKEDVDLLMLPMTAEGKEPTSSMGNDTPLAVFSEKPQRL
                     FNYFRQQFAQVTNPPIDPIREELVMSLTGYVGSIHKNLLEPVSDLCKMVKLKSPVLTN
                     TQFDILLNLRYKGFSTAVLPMLFNPKKGADGLVKAVDELCLLVEKAVDEGKNYIVLSD
                     RGVDEEHAPIPSLLAVSAVHHYLIDKRKRMQTDIIVESGEPREVMHFALLFGFGANAI
                     NPYMAFAVISERIKSDDIKLDFHTAESNYVKAVNKGLLKILSKMGISTLRSYRGAQIF
                     EAVGISSSVLNKYFKGVISKIEGIDMTDLANETLFPYWKAFYPEIDEENRLKNLGFYS
                     FRVEGEKHAWNPETIARLRIATRTGDYAKFKEYSSIIDNKPEAIFIRDLLDYKRNPID
                     VNEVEPLENIMKRFVTGAMSYGSISKEAHEALAMAMNKIGGRSNTGEGGEDPERYKQR
                     PDGLSTRSAIKQVASGRFGVTAEYLVNADEIQIKIAQGAKPGEGGQLPGYKVDNVIAK
                     TRHSIPGISLISPPPHHDIYSIEDLAQLIFDLKNINPAAMISVKLVSESGVGTIAAGV
                     AKAKADLILISGAEGGTGASPASSIKHAGLPQEIGLAEVQQTLVINNLRGQIRLQTDG
                     QLKTGRDIVISALLGAEEYGFATSALIVLGCVMMRKCHINTCPTGVATQNDELRKHFN
                     GRYEYLINFFTFLAQEVREHLAQMGYRSLDEIIGHTELLVRKEIKTSSKIEKVDLSKI
                     LFKPKQAEENAIRKTKNQEHKIDQVLDRILISEAMPAIEQCIPVKIRHKVRNTDRTIG
                     AMLSGEIAKRYGQVGLPEDTVQIQMEGSAGQSFGAFLSKGITLRLEGDANDYLGKGLS
                     GGKIIVVPPKESTFAPEKNIIAGNTLLYGATSGEVYINGIVGERFCVRNSGAIAVVEG
                     AGDHCCEYMTGGRTVVLGKTGRNFAAGMSGGIAYVLDEDGKFDMFCNMEMVELSLIEE
                     IADNKELHEFISKHFKYTGSPLAKRILDNWSEYSEKFLKVTPIEYKKVLQEEKIQAIN
                     KKIEQVERDY"
     gene            complement(8360..10558)
                     /locus_tag="PLD44_06845"
     CDS             complement(8360..10558)
                     /locus_tag="PLD44_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOF99085.1"
                     /translation="MSSLRFKAVEEAFKKKAAAVEVPSELTSEYFGKYVFTRSTMARF
                     LSEQTIKEIDEAIEKGTTLDRSIADKVAEGMKKWAMELGATHYTHWFQPLTDKTAEKH
                     DSFIDYLDDKPGENIIEKFSGKLLAQQEPDASSFPSGGIRSTFEARGYTAWDPSSPAF
                     IVDDTLCIPTIFISYTGESLDYKAPLLKSLNSVDKAATAVCKLFDPKVKKVFSYLGWE
                     QEYFLVDENLYVTRPDLLLTGRTLLGHESAKNQQLEDHYFGSIPTRVVAYMKDLEFEA
                     YKYGIPLKTRHNEVAPNQFELAPIYGECNLSVDQNLLIMSIMKKVARRHGFRLLLHEK
                     PFAGINGSGKHNNWSLGTDTGVGLLTPGKTPEENLQFITFVVNILMAVYKHNALLKAS
                     IMSAQNAHRLGANEAPPAIISVFLGTQVSSVLDKLENSSSDEAIVVDSKKKLSLGIAH
                     IPEVLLDNTDRNRTSPFAFTGNRFEFRAVGSSANCSSAMTALNTAVAAQLIEFKAEVD
                     KKIASGISKQTAIFEVLKEYIKISKPIRFDGNNYSDEWKEEAKLRGLDCETSVPKILK
                     AYTNEESIKIFISTNVMSEKELHARNEVKWDMYTKKIQIESRVLGDLAINHIIPIATR
                     YQSILLDNVNKMKTVFSKEEAEKMCQLDLQLIKEIAQRVNTIKSDVDNMIEARKKANV
                     IECEEEKANAYHDTVLPFFDKIRYQVDKLEMIIDDEMWPLPKYRELLFIR"
BASE COUNT         3162 a         2435 c         2018 g         3475 t
ORIGIN      
        1 atagaaggct ctaatgagag ggtaaccgag caatggattg ggaacgcccg aaatttaatc
       61 gtaactcaaa tggagtaaat caggcaagac gcagcgaaag tttaatatct tatcccgaga
      121 ggtcttatat ggtgcatcag cagtgcaaga aatgcaacga tgtattttac accttacaag
      181 aagtcagcag gggacatagt attctgttga aaaacagaag aaggtctgaa tcttttaatt
      241 taaggagcag ttagttcaaa atttgtttta tggagcagca gcaagcaata acgtaccaat
      301 acgacctgtt tgattcaaag ggtcaggatg ttattcgttc tttcgcaggg agtgaagccg
      361 taagcggagc tcaggcgata aaggcgttgc aagtaaaaga agcaggcgaa cagaaacgag
      421 ccataacgca cgatttgatg ggagggtggg ggagtaattc ccctacctac tcgattaact
      481 ctgcattaaa taagattgtt tgcaagcagc agatcaacaa cgaaaaaatc aataaccttc
      541 cttgtgaata ctctttaccg agcgtccaga aggatcggcc atatttttga atgaagcatc
      601 ccactcgaga gcaatgggtg tgctgcatgc aatagaggga accgaaggca cacaaagcgc
      661 tgctgaatcg gacgggaaca aatccgagta aatggaacgg taataatatt cttccttagt
      721 catgggaggc tgaatgggaa atttttcggc tgccttttcc atttcttcgt cgcttaccaa
      781 cttgttggtc atttctttta atgaatcaat ccagttgtaa cccacaccat ccgaaaactg
      841 ttccttttgc cgccatacga ttttctcggg caaataattc tcaaatgctt tacgcaaaat
      901 ccatttttct atttttccgt ttccggccat tttatctttt ggatttaacc gcatggctac
      961 atccaaaaat tctttgtcca aaaaaggaac acgaccttct acaccccacg acgccaacga
     1021 tttattggct ctcaaacaat cgtacaaatg cagattcttg atttttctga cggtttcctt
     1081 gtgaaattct tcgggatttg gagctttgtg aaaataaaga taacctccaa aaatttcatc
     1141 tgaaccttca ccggaaagaa ccattttaac gcccatcgat ttaatgaaac gtgccagcaa
     1201 atacatgggt gtactggctc tcacggtagt tacgtcataa gtttcgatat gataaataac
     1261 atctcgtaaa gcatccaatc cttcctgaac ggtaaaatga atttcgtgat gaatggtgcc
     1321 aatatgttcg gccacttcac gtgctgcagc caaatcgggc gaaccttcaa gtccaatggc
     1381 aaacgaatgc agctgcggcc accacgcatc ctgagtattt ccactttcga tacgcttggg
     1441 cgaaaatttt tttgctattg ccgaaattac ggaagaatct aaacctcccg aaagcaatac
     1501 gccgtaggga acatcggaca taagctgacg ttcgacggct gcttcaagtg ctttgcgcaa
     1561 ttcttcaata ttgctttcgt tgtctttcac attttcataa tcttcccaat cacgatggta
     1621 ccattgtttg ggttgcccgt ccaaactgta aaagtactgt ccaggaagaa attcttcgat
     1681 acggttgcaa taaccttcca aagctttcaa ctccgaagca acaaaataag ttccatcttt
     1741 gtcccaaccc tgataaagtg gaacaatacc aatgtgatca cgtccgataa agaaagcatc
     1801 tttttcaata tcgtagagtg caaaagcaaa tattccattc aaatcattca agaaatcggg
     1861 gcctttttcc cgataaagcg ccaaaattac ttcacaatcc gatttggtaa gaaattcgta
     1921 ttttccatcg aactgtttcc gtatttcctg atgattataa atttcgccat ttacggcaag
     1981 taccaatttt ccatctttac tgtacaaggg ctgcttgccg gattcgggat caacaatcga
     2041 caggcgctca tgcgccaaaa ttgcccggtc attgctaaaa atgcccgacc aatcaggtcc
     2101 gcgatgtctt actctctttg acatctgcaa aacttgagga cgcagttctt gggaattctt
     2161 ttttatatca aaagcgcaaa ctattccgca catatttctc taatttttta tgaaaaaaaa
     2221 acattttctt tgattcattt tttctgctca tggcagaaaa aatggtgtat attttcggct
     2281 gcttttcgtc ctgaagctat ggctctaact accaaacttg ctcccattac cgaatcaccg
     2341 gtagcaaaaa ttttatccac cgaagttttc ccgtttttgt caacttcaat atttcctctg
     2401 ccatcatatt ttacacccaa ttcgttaaga aagccttcgt gtaccggatg cagaaatccc
     2461 aatgccagta aaaccaaatc agccttaacg gtttccgttt tccccgtaga aaccattgtc
     2521 atttgtccat tttcttctct gtgccattct acttcttcaa ttaagacttc cgtaacgtgt
     2581 ccatttttcc cgataaacgc ttttgtattc aggcaccaac gccttgtaca gccttcttcg
     2641 tgtgaactgg aagtttttaa aatcaacgga taataaggcc agggcgtttc cggatttttt
     2701 ccaatcggtg gttgtggtaa aatttctatt tgcgtaactt ttacggcacc ttgtctgatg
     2761 gatgttccaa cacagtctga gccggtatcg cctccaccaa ttaccaacac atgtttgttt
     2821 ttggccgaaa taatttcttc ctgttgcatt gtttctccaa gcaactgttt gttttgctga
     2881 ctcagaaaat ccattgcaaa ataaattcct ctcatttctc ttccttcggc ctgaatatca
     2941 cgtggttgtc ctgcaccaac agctatgcag attgcatcaa aactgtccaa cagttcctta
     3001 gctgaaatat ttttcccaac ttccatattg ggactaaata tgattccttc ctcttccatc
     3061 aacttcaatc gcctatcaat tacatttttt gccaacttaa aattaggaat gccataacgc
     3121 aacaagcctc caatatgagc atctttttca aaaaccgtaa ccgtgtatcc ctttcggttc
     3181 aattgctggg cagcagccaa accagacgga ccagaaccaa taacggcaat ttttttcgct
     3241 atccgttttc ttggaggacg ggctttaatc atgccttcat taaaagctaa ttcggtaata
     3301 gcagcttcat tttcacgaat cgttaaaggt gcttcatgca aagacagcac acacgatttt
     3361 tcacaaggag ccggacaaac tcttcctgta aattcaggga aattattggt ttcctgcaaa
     3421 atatcaatgg cttcctgcca tttctctttg taaatcaaat cctgccattc gggcattttg
     3481 ttcccgagcg gacaagccca atgacaaaac ggaactccac aatccatgca tctcgaagct
     3541 tgaagtttcc tgtcttcacg attcagggtt tgttccactt ctccaaaatc gtaaattctt
     3601 tcctgaacgg gccgataacc ggcctcctta cgcggaatat ataaaaatgc tttcgggttt
     3661 cccataattt tgaaatttaa acttttattg aacaacacgt tacatcaata atctctttca
     3721 acctgctcga tttttttatt gattgcctga attttttctt cctgcaacac tttcttgtac
     3781 tcaatgggag taactttcag gaatttttcg gaatattccg accaattatc caaaatacgc
     3841 tttgccaaag ggcttcctgt gtatttgaaa tgtttggaaa taaactcgtg aagttccttg
     3901 ttatcagcta tttcttcgat aagagaaagt tccaccattt ccatgttgca aaacatatca
     3961 aatttgccgt cttcatccag cacatatgct attcctccgc tcatccctgc ggcaaagttt
     4021 ctacctgttt ttcccaacac aacggttctt cctcccgtca tgtattcgca gcaatgatca
     4081 ccggcacctt ctacgacagc aattgctccg gaatttctta cacagaaacg ttcgcctaca
     4141 ataccattga tatacacttc acccgaagta gctccataaa ggagtgtgtt gccggcaata
     4201 atattttttt cgggagcaaa agtactttcc ttcggaggaa caacaatgat tttcccgccc
     4261 gaaagacctt tcccgagata atcgtttgca tcgccttcga gacgcagcgt aatgccttta
     4321 gaaagaaaag cgccaaaact ttgtcctgca gaaccttcca tctgaatttg aacggtatct
     4381 tccggcagtc caacctgtcc ataacgtttg gctatttctc cggaaagcat agctcctatg
     4441 gttcgatcgg tatttctgac tttatgtcga attttaacag gaatacattg ttcaatggcc
     4501 ggcatggctt cggatattaa tattctgtcg agcacctgat caattttgtg ttcctgattc
     4561 tttgtttttc tgatggcatt ttcttctgcc tgcttcggtt taaaaagaat tttggacaaa
     4621 tcaacctttt caatttttga actcgtttta atttctttgc gtaccaacaa ttcggtatgt
     4681 ccgataattt catccagact tctatagccc atttgagcca aatgttctct cacttcctgt
     4741 gccaaaaagg taaagaaatt tatcagatat tcgtatcttc cgttgaaatg tttgcgaagt
     4801 tcgtcgtttt gtgtggccac acctgtagga caggtattta tatgacattt tctcatcatg
     4861 acacatccca aaacaatgag ggcgctggta gcaaaaccat attcttcagc tcccaacaaa
     4921 gcagaaatca caatatcccg tcccgttttt aactgaccat ccgtttgcag tcggatttgt
     4981 ccacgcaaat tattgatcac caacgtttgt tgaacttctg cgagaccaat ttcctgtgga
     5041 agtccggcat gtttaatgga actggcggga cttgctccgg taccaccttc tgctccgctg
     5101 ataagaatca aatcggcttt tgcttttgcc actccggcag caatagtacc cacaccactt
     5161 tccgaaacaa gttttacgct aatcatggca gccggattga tattttttaa gtcaaaaatc
     5221 aattgggcaa gatcctcaat agaataaatg tcgtgatggg gcggcggaga aattaaagaa
     5281 attccgggta tggaatgtcg ggtttttgca ataacattat cgactttgta tccgggaagt
     5341 tgtccgcctt caccgggttt tgcaccttga gctattttaa tttgaatttc gtcggcattt
     5401 accaaatatt cggccgtaac tccgaatctg ccggaagcaa cctgcttgat ggcgctgcgc
     5461 gtacttaaac cgtcaggtcg ctgtttataa cgttcaggat cttcgcctcc ttcaccggta
     5521 ttgcttctac ctccgatttt attcattgcc attgccaatg cttcatgcgc ttccttgcta
     5581 atagaaccat aggacatggc tcctgttaca aatcgcttca taatgttttc taaaggctca
     5641 acttcattta catcaatggg attgcgcttg taatcgagta aatcgcgaat aaaaatggct
     5701 tccggtttgt tgtctataat gcttgaatac tctttgaatt ttgcataatc tccggttcgc
     5761 gtagcaatgc gcaaacgggc aatggtttcc ggattccatg catgtttttc tccttctacg
     5821 cgaaatgaat aaaaaccaag attcttcaaa cgattttctt catcaatttc aggataaaaa
     5881 gctttccaat aaggaaataa agtctcattg gccaaatcgg tcatatctat tccttcgatt
     5941 ttggaaatga ctcccttgaa atatttgttc agcacggatg atgaaatacc tacagcttca
     6001 aatatctgag cgccgcgata acttcttaag gtagaaattc ccattttcga aaggattttc
     6061 aaaagcccct tgttcacagc tttaacataa ttcgattcgg ccgtatggaa atcgagtttg
     6121 atatcgtccg atttgatgcg ctcgctgata acggcaaaag ccatataagg attgattgca
     6181 ttggctccaa aaccaaacag caaggcaaaa tgcatcactt cacgcggttc tccactttcg
     6241 acaataatat cggtttgcat tcgtttccgc ttgtcgatga gataatgatg tacagcagaa
     6301 accgcaagca gcgaaggaat gggagcatgt tcctcgtcta cacccctgtc gctcaacaca
     6361 atataatttt tcccttcatc caccgctttt tctaccaaaa ggcaaagttc atctactgcc
     6421 ttcaccaaac catcagctcc ttttttcgga ttaaaaagca tcggtaacac tgcagtagaa
     6481 aaacctttgt aacgtaaatt aagcaaaata tcgaactgag tattggtcag aaccggactt
     6541 ttcagcttta ccattttaca aagatccgaa acaggttcga gcaaattttt atgaatcgag
     6601 ccaacgtaac cggttaaaga catgaccaat tcttcacgaa tcggatcaat gggtggattg
     6661 gttacttgag cgaattgctg acggaaataa ttgaataacc gttggggttt ctcggaaaaa
     6721 acagccaaag gggtatcatt tcccatcgat gaagtaggtt ctttcccttc agcggtcata
     6781 gggagcatca gcaaatcgac atcttccttt gaataaccga aagcgataag cagattctga
     6841 taatttttta tttccggctt tacacttctg cccgacgaaa tttcctccag attgattctg
     6901 ttcctttcca accattcgtt gtaaggaaat gctttggcaa tttgttcctt taactcttcg
     6961 tcataataaa ttttgccttc ttcagtatcc accatcagaa ttttaccggg tttcaatcgt
     7021 cccttttctt taatattttc cggttcaaat tctattgtgc cggtttcgga ggcaataatc
     7081 atcataccat cattggtaat aagatatcgg gccggtcgca gaccgtttcg gtcaagcatg
     7141 ccgccggcgt agcgtccatc gctgaaaaga agtgtcgccg ggccatccca cggttccata
     7201 aacatgctgt ggtattcata aaaacctctc agttcttcgg aaataggatt cttttgattc
     7261 caactttctg gtaccaacat ggccaatgcg tgaggcagac tttttcctgc cataacgaga
     7321 aattctaaaa cattgtccag agaagcactg tcgctcattg caggctgaac gatgggaaac
     7381 aaaggtttta aatcgcccag caactccgat ttgaatacac tttcacgcgc ttccatccat
     7441 aatcggttgc ctctcactgt attaatttca ccgttatgag ccaacatcct gaaaggttga
     7501 gccaaatccc aggtcgggaa agtgttggta ctgaaacgtg aatgaaccaa agcaatggca
     7561 ctggtaaaat tgggattggt caaatcggga taatagtcac gcaattgaag tgatgtgagc
     7621 attcctttat aaatcagtac tttggaagaa agacttacta tataaaagct ccttctgttg
     7681 gaaagattgg attttgctat cgaattttca atttttttcc ttaccagata gagttttctt
     7741 tccaactctt cctgacgaag attaccggta acaaatattt gctttatcag cggttcgttg
     7801 gatgcagaaa tttcacccaa aattttgctg ttgaccggca tatcacggac tgccaacaaa
     7861 tgaagatttt cttcttcaac attttcctta ataatacgta aacaaagttc tgattcctct
     7921 ttttctcgtg gcagaaatat aagaccggtg ccatattttc cgatagcggg gactggaatg
     7981 ccttgtagca aaataaattc gtgaggaatt tgcagcagca taccggcacc gtcgccggtc
     8041 ttgttatcgg cactttctgc tccgcgatga accatattct cgagcacttg taatccattt
     8101 tcaatgattt cgtgggattt ttcaccggaa atatttacca ctaaaccgac tccgcatgca
     8161 tcatgctcat tctgaaaaga atacaatgat tgctgctcgt ataaatttaa ataattcata
     8221 atcaattttt taattttata gagatgggtt caaaatttgt caactgcaaa ataacagttg
     8281 acaaattttg aaggtagaaa aaactgtgtg caataaaaaa cctaagaagc ttattaataa
     8341 aggatttttc gggtaagact tagcgaatga aaagcaattc tctatatttt ggtaaaggcc
     8401 acatctcgtc atcaataatc atttcgagtt tgtccacctg ataacgaatt ttgtcgaaaa
     8461 atggtaagac ggtatcgtga taagcattgg ccttttcttc ctcacattca attacattgg
     8521 ctttttttct ggcctcaatc atattatcta catccgactt gatagtgttc actctttggg
     8581 caatttcctt tatcaactgc aaatcgagct ggcacatttt ctcggcttct tcctttgaaa
     8641 ataccgtttt cattttgttg acattatcca gcaaaataga ttgataacgt gtagcaatcg
     8701 gaatgatgtg attgatggcc aagtcgccca acacacgaga ttcaatctga attttctttg
     8761 tgtacatatc ccatttaact tcatttcgtg catgaagttc cttttcggac atgacattag
     8821 tagaaatgaa aattttgata ctctcttcgt tggtataagc tttcaaaatt tttggcacac
     8881 ttgtttcgca atccaaccca cgaagttttg cttcttcctt ccattcgtcg ctatagttgt
     8941 tgccatcaaa acggatgggt tttgatattt ttatgtattc tttcaacact tcaaaaattg
     9001 cagtttgctt actgattcca gaagcaatct ttttatccac ttcagcttta aattcaatta
     9061 attgtgcagc tacagctgta tttaaggctg tcattgccga agagcaattg gctgaagaac
     9121 ctacggcacg aaattcaaac ctgttgcctg taaatgcaaa aggcgaagtt ctgtttctgt
     9181 cagtattgtc gagtaacact tcgggaatat gggcaatacc caaactcagt tttttcttgc
     9241 tgtccactac aatggcttca tcgctgctgc tattctcaag tttatccagc acactgctca
     9301 cctgtgtacc aagaaaaaca gaaataatgg ccggtggtgc ttcgttggct cccaagcgat
     9361 gggcattttg agccgacatg atggaagctt tcaacaacgc attgtgtttg taaacggcca
     9421 tcaaaatatt tactacaaaa gtgataaatt gaagattttc ttcaggagtt ttaccaggag
     9481 taagcaagcc aactcccgtg tcggttccca gcgaccaatt gttgtgtttt cccgaaccgt
     9541 ttattccggc aaaaggcttt tcgtgaagca acaaacggaa gccgtgtcga cgagcaactt
     9601 ttttcataat ggacataatt aacaaattct gatccactga caaattgcat tctccgtaaa
     9661 tgggtgccaa ttcaaactga ttgggtgcta cctcattgtg tcgggtcttc aaaggtatgc
     9721 catatttgta agcttcaaat tccaaatctt tcatataagc taccacccga gtaggaattg
     9781 aaccaaaata atggtcttcc aactgctgat tttttgccga ctcgtgtccc agcaaggttc
     9841 ggccggttag caataaatcg ggacgggtaa catacagatt ttcatctacc agaaaatatt
     9901 cctgttccca acccagataa gaaaaaactt ttttcacttt tggatcaaac aatttacaaa
     9961 ccgctgtagc tgccttatcc accgagttga gcgattttaa taaaggagct ttgtaatcga
    10021 gcgattcgcc ggtgtaagaa atgaaaatgg tagggataca caacgtatcg tcaactataa
    10081 aagcaggcga tgaaggatcc cacgccgtat aaccgcgcgc ttcaaaagtg cttctgattc
    10141 ctccgctcgg aaaagacgaa gcatcaggtt cctgttgcgc caacaacttt ccggaaaatt
    10201 tttcaatgat gttctctccg ggtttgtcat ccaaataatc aataaacgaa tcgtgctttt
    10261 ctgccgtttt atctgtaagc ggctgaaacc aatgtgtata atgagttgcg cctaattcca
    10321 ttgcccattt tttcatccct tcggcaactt tgtcggcaat actgcgatcg agtgtggttc
    10381 ctttttcaat ggcttcatca atttctttta tagtttgttc cgaaaggaat ctggccattg
    10441 tggatcttgt aaaaacatac tttccaaaat actcggaggt taattcggaa ggtacttcca
    10501 ctgcagcggc ttttttcttg aaagcttcct caactgcttt aaatcttagt gatgacataa
    10561 atttataatt ttgaatttat tctgtaattt ttatcttaaa aattttctta ttgctaactt
    10621 aatgttatta tttctctgca aaattaaata aaatgataat ctcaatctat gaaattcatg
    10681 tttttttgaa aaaatatttc taaaacttac tttttgaaac gattagttat aataaaaaga
    10741 agaattataa gaaaatctat agaaaaagtt atatattaaa agtttaggag ttttcttctc
    10801 cgaataaatt gagttgaagc caatactgaa aaaatttgaa caaagataga taaaagtttt
    10861 cttatttttc tttaagactt tgattcatat tgtctactga ctgaatgata aaaggtgtaa
    10921 gcatcaaaaa atttcaactt tgcatcgaac gatctctatt cggatcatag aattttattt
    10981 attcaatttt ttacctgaca ttttgttgtt aaaattcgaa ttattgtttc ttaatgaaat
    11041 tttttcgtag aaaacttcat ttcaccgttt ctaaaaaact tgttttaaaa
//
LOCUS       DAOSIB010000071        10817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_5981_length_10817_cov_12.022579, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000071 DAOSIB010000000
VERSION     DAOSIB010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5981_length_10817_cov_12.022579"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..770
                     /locus_tag="PLD44_06850"
     CDS             315..770
                     /locus_tag="PLD44_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOF99086.1"
                     /translation="MEKIDELDRQILKIITQNARLPFRDVAVQCGVSRAAIHQRVQRL
                     IDMNVITGSGYFVNPKKLGYQMCVYIGITLERGSMYKEVVQELEKIPEIVESQYTLGP
                     YTILVKLYAKNDEHLMELLNGKIQEIKGVATTETLTSLDQRIRRTIPID"
     gene            824..1852
                     /locus_tag="PLD44_06855"
     CDS             824..1852
                     /locus_tag="PLD44_06855"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOF99087.1"
                     /translation="MFAFKVIHAAFYFFADMKVLVTGATGLLGHQVTMELIQRGYQVQ
                     IIVRKTDNIFFDLNAVKVFIGNFTEYEHLKAAASGCEAVIHIAAVTDVHLLHYEDYRK
                     INVEGSAQVIRVCRDLNIRNVVYISSSNTIGFGSKSKPATEDFPFQYPFTRSFYARSK
                     VEAEKLFQQFASEFGNHVIIIHPTFMIGAYDVKPSSGKLLLMGYRKLLLFIPKGGKNF
                     VPVRDVAKAVCNALTLGKNGEHYLTAGVNLSFAEYYRLQKNIAGYRQIIMEVPDFLLK
                     VLGKVGDFLRFLGIKTDVCSMNLSQLMIREYYCGKKAIDELNMPQTDLKLAIAEAVEW
                     FKERGWIR"
     gene            complement(1899..>2630)
                     /locus_tag="PLD44_06860"
     CDS             complement(1899..>2630)
                     /locus_tag="PLD44_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HOF99088.1"
                     /translation="EKNEEKKKKSTSETVKNVSSLLWQFVQTQQSTIGENSSMRKDDE
                     NKIYEWGEATYAPQKEIISSTKSEKFVQYPSCNSAQKLQIRHQGSQYLIEEKDLSDSQ
                     LNYGIIMHEILRKITNQADQDKVLLEFVSSGQISQAEKITVEKGLEKFWSLPQAKEWF
                     NGEGRILNETTILSPENRLHRPDRVIIQEAKATVIDYKFGQKESKYHLAQVKEYMDLI
                     AQMGYQTKGYVCYAELGKVVEVFQN"
     assembly_gap    2631..2730
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2731..>4466)
                     /locus_tag="PLD44_06865"
     CDS             complement(<2731..>4466)
                     /locus_tag="PLD44_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOF99089.1"
                     /translation="NCTNKSTPNWVKNKVNSAYISGLGKCFEKINEIFQEPFIRYNSV
                     DIVLKHIYKLGILSDLAMEINQLTAEQNTFLISDTNLLLNRIIDNSDTPYVYEKIGNK
                     LQHFMIDEFQDTSVLQWKNFLPLVRNSLAENNFNMVVGDVKQSIYRWRNSDWKLLDEQ
                     IFKDFTPQQIRQEPLNVNWRSDRNIVKFNNNFFSQSAQLLQQQLNANIQEYLPADKYP
                     FVSELSNKIQHAYTDTEQKVSEKAGAGHVCIQFIPNNKKNWQEESLNRLPIMLENFQD
                     RGYKASDIAILVRDKKDAALVIESLMNYKNSPSAKEGYCYDILGNEGLLIGKASSVRF
                     IVGLLKLFVSPSDDIQLTIVSLEYALNKLGKTKNEALRDIAALKKNTENKFSHLFDSE
                     EEKALQAIRHYSLFDRVEQIISLFKIGEWENQAPFVQAFQDLVYNFTAKGFSDISDFL
                     SWWDEAGCNKFVAMPADQEAIHIMTIHKAKGLDFPVVILPLCDWALEGNKHKTNVLWC
                     ETNVPPFSELPLLPISYNQYLRQSIFTQNYFDEQMHLYIDNLNLAYVAFTRPKNELVC
                     MAPAPDEKNEEK"
     assembly_gap    4467..4566
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4567..4974)
                     /locus_tag="PLD44_06870"
     CDS             complement(<4567..4974)
                     /locus_tag="PLD44_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOF99090.1"
                     /translation="MLNVYRASAGSGKTFRLTKDYIHLLFRHQGENLHRHILAVTFTN
                     KATEEMKSRILKELFALAQGQPSNYRKSLMEEYGRNEAEVNLKAKQILVSLLYDYSSF
                     AVSTIDSFFQQVIRSFAREMGINGAYNLEMENDA"
     gene            complement(5013..5345)
                     /locus_tag="PLD44_06875"
     CDS             complement(5013..5345)
                     /locus_tag="PLD44_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOF99091.1"
                     /translation="MDWKEKLNQLKDSLPQDNSEPTENELPEKKVQTEPLRVQMEKRN
                     GKPVTIVSEFEGNEKELKELAKTLKVSCSAGGTAEHGMVLIQGDFRVKIAEILQKMGY
                     KVKKINFK"
     gene            5875..6687
                     /locus_tag="PLD44_06880"
     CDS             5875..6687
                     /locus_tag="PLD44_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOF99092.1"
                     /translation="MKEVTKVLYISQEIFPYLPASEMANMGRYLPQAVQEKGLEVRIF
                     MPKFGSINERRNQLHEVIRLSGMNIIIDDTDHPLIIKVASIPAARMQVYFIDNEDYFQ
                     RKNTVANENNEEYDDNDERAIFFARGVMETVKKLRWSPDVIHCQGWITALVPLYVKKI
                     YNDDPFFKHSRVVYSIFDDEFKKPLNDRLMDKILMDGIQKKHLASIKNPPVDYHSLLK
                     LAADYSDGFIQSSPKVNESVLKYVADKKIKFLPYQPAEDYSDQYIDFYNQLF"
     gene            6718..8088
                     /locus_tag="PLD44_06885"
     CDS             6718..8088
                     /locus_tag="PLD44_06885"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HOF99093.1"
                     /translation="MHFNKFFLFLFTLAFVSCTDGLTDIGVSIMPSQDIISVNADAFD
                     LNTENVFVDFIYTRQDSFLLGNFYDHKYGSTRADILAQVNCPVDFTFPDNAVPDSAVI
                     ALRYLTWFGDKYSPMEIKIYQMNKATFSYSEHYPSNINPDDYTDRSILLGKRIITPSD
                     TSGSRSDGTQVRFKLSDSFVQWFFPENRTYNSQKDFLQKFKGMFITTTFGSANILYVN
                     QIDLYYYYHYTYVRKTSDGSDSTVTVNNVITFPANAEVRQVNRIQHPDRNEVVQSRPE
                     VNYVASPANINTRISLPVDKIAKQMKTTIHNKYLSVNSALLKVEVTEQDEATIAVPLV
                     QNMMLIKESALDRFFKNSELPSDTCALIAQYSYTKNADTNSYDYYYTFNLSKLIANEI
                     KKAETNGTQLPETMDLLLVPVTVQYNNSGYYTSVKHQILLSGVTIKSGKNTSSPMKIN
                     LVYSGF"
     gene            complement(8102..9415)
                     /gene="der"
                     /locus_tag="PLD44_06890"
     CDS             complement(8102..9415)
                     /gene="der"
                     /locus_tag="PLD44_06890"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOF99094.1"
                     /translation="MGNIVAIVGRPNVGKSTLFNRLTQTRRAIVNETAGTTRDRQYGK
                     SEWSGYEFSVIDTGGWVTNSSDTFEQEIKRQVQLAINEADVILFLVDVQCGITDLDFE
                     VAQLLKKTNKPVILVTNKADTFEWQYQAPEFYKLGMGDPFLVSSINGLGTGDLLDKLV
                     SYLKPQEDEETEEHLPRFAIVGRPNAGKSSLLNSFLDEERNIVTEIPGTTRDSIYTRF
                     NKFGFNFYLVDTAGIRKKAKVDEDLEYYSIVRSIRAIENSDVCMLLIDATRGLESQDL
                     NIFSIIQKNKKGIVVCVNKWDLIEDKDTHSTKKMEAYIRERMSPFTDFPIIFISALTR
                     QRIFKVLESAVKVYENKNKKIPTAKLNEYLLPLIQNFPPPALKGKYIKIKYVTQLPDT
                     QVPTFIFFANLPQYIKEPYRRFLENKIRSQWNFTGTPIQLFFRKK"
     gene            complement(9628..>10817)
                     /gene="thrS"
                     /locus_tag="PLD44_06895"
     CDS             complement(9628..>10817)
                     /gene="thrS"
                     /locus_tag="PLD44_06895"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOF99095.1"
                     /translation="FMFSEMVGKGLPIWLPRGTDLRLRLENVLKTIQQRFEYQQVITP
                     HIGNKQLYVTSGHYAKYGKDSFQPIHTPEEGEEYLLKPMNCPHHCEIYKFKPRSYKDL
                     PLRFAEFGTVYRYEQSGELHGLTRVRSFTQDDAHIFCRPDQVKDEFLKVMDIIFIIFN
                     ALDFKNFEAQISLRDPDNKEKYIGTDENWEKAEKAIIEACEEKGLKAKIVQGEAAFYG
                     PKLDFMVKDAIGRKWQLGTIQVDYNLPERFELEYTGEDNQKHRPVMIHRAPFGSMERF
                     IAVLIEHTAGKFPLWLTPDQVVVLPISEKFNDYAFDIAKKLKEKSIRVTVDERNEKIG
                     RKIRDNELKRIPYMLIVGEKEMETSHVSVRRQGEGDAGSMSVEEFAELIHNEVVSQMK
                     QFN"
BASE COUNT         2965 a         2041 c         2100 g         3511 t
ORIGIN      
        1 taaaaaataa attatattct gatgaaatga aaaaagtgat gtacaattag atttttttct
       61 ccgggaaaac aaatacgttg tgaactcttg aaagttgcca ctctttttta aattttcctg
      121 aattttcgga tttttctgaa attattaccg atattttaat cattgaaaag acttcctttt
      181 gtcaaaaagt ttctattttc tattaaaagg atattatttt taattcttga gataaatttt
      241 gccgacaatt tttcagaatt cgagtttttc tatttatttt tgttgtctaa atgatacaat
      301 aaaattttat agccatggaa aaaattgatg agttagacag gcaaatttta aagataatta
      361 cgcaaaatgc gcggttgccc tttcgcgatg tagctgttca atgtggagtt tcacgagcag
      421 ccatacatca gcgcgtacaa aggctgatag atatgaatgt cattaccggt tcgggatatt
      481 ttgtcaatcc caaaaaactc ggttatcaaa tgtgtgttta tatcgggatt actcttgaac
      541 gcggctccat gtataaagaa gtggttcagg aactggaaaa aattcctgaa attgtcgaat
      601 cgcagtacac gttagggccg tacaccattt tggtaaaact gtatgcaaag aatgatgaac
      661 atttaatgga attgctcaac ggaaaaattc aggaaataaa gggagttgca actaccgaaa
      721 cgctcacttc actcgatcaa cggattagac gtacaattcc tatagattga acgatcgggg
      781 gaaactgctt taaagtcaat caacctattt ttatttttgc agcatgtttg cttttaaggt
      841 gattcatgct gctttttatt tttttgccga catgaaagtt ttggtaacag gagcaaccgg
      901 tttgttggga catcaggtaa ctatggaatt gattcagcgt ggttatcaag tgcaaattat
      961 tgtccgaaaa acggataata tttttttcga tctgaatgca gttaaggttt ttatcgggaa
     1021 ttttacagaa tacgagcact taaaagctgc ggcttcgggg tgtgaagccg tgatacatat
     1081 cgcagcagtt accgatgtgc atttactgca ttatgaagat tatcggaaaa taaatgtcga
     1141 aggttcggca caggtaattc gtgtttgcag agatttgaat attcgcaatg tggtgtatat
     1201 cagttcttcc aataccatcg gttttggttc aaaaagcaaa cctgccactg aagattttcc
     1261 ttttcaatat ccttttaccc gctcttttta cgccagaagt aaagttgaag ctgaaaagct
     1321 gttccagcaa tttgcttcgg aatttggtaa tcatgtaatc attattcatc ccacatttat
     1381 gattggagct tatgatgtaa agccgagttc aggaaaattg ctgctgatgg gttatcgaaa
     1441 gctactcttg tttattccga agggagggaa aaattttgtt ccggttcgcg atgtggctaa
     1501 ggctgtatgc aatgctctca cattgggtaa aaatggggaa cattatttga ctgccggcgt
     1561 taatctttca ttcgcagaat attatcgttt gcagaaaaat atagccggtt accgccagat
     1621 cataatggaa gtcccggatt ttttgttgaa agtgttggga aaagtcggag attttcttcg
     1681 ttttttaggt atcaaaacgg atgtttgttc catgaatctt tctcagttga tgattcggga
     1741 gtattattgc ggaaaaaaag ccatagacga gctcaacatg cctcagaccg atttgaaact
     1801 tgctattgct gaagctgtcg aatggttcaa agaaagaggt tggatacgct gaaaagagag
     1861 aattctcaat tttttttgtg ggcgaaaaag tttttttgtc agttctgaaa aacttcaacc
     1921 acttttccca gttctgcata acaaacgtaa ccttttgttt gataacccat ttgagcaatt
     1981 aaatccatgt attctttcac ttgtgcaaga tggtatttac tttctttctg accgaatttg
     2041 taatcaatca cagtagcttt tgcttcttga atgatcactc gatccggacg atgcagacga
     2101 ttttcgggtg aaagaatggt tgtttcgttc agtattctgc cttcaccgtt gaaccattct
     2161 tttgcttgtg gaagcgacca gaatttttcc aatccctttt caaccgttat tttttctgct
     2221 tggctaattt gaccggaaga tacaaattcg agcaacactt tatcctgatc tgcttggttg
     2281 gtaattttcc gtagaatttc gtgcatgatg atgccgtagt tcagctgact gtcggacaaa
     2341 tctttttctt cgatgagata ttgacttcct tgatgccgaa tttggagctt ttgtgcactg
     2401 ttgcacgaag gatattgaac aaacttttca gattttgtgg atgaaattat ttccttttga
     2461 ggtgcatacg ttgcttctcc ccattcgtaa attttatttt catcatcctt tcgcatcgac
     2521 gaattttctc caatagtgct ttgttgtgtt tgaacaaatt gccaaagtaa ggaagaaaca
     2581 ttttttacag tttcagacgt agattttttc tttttttctt catttttttc nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tttttttctt catttttttc atcgggtgcc
     2761 ggcgccatgc aaacaagttc gttttttgga cgagtgaatg caacgtaagc aagattcaga
     2821 ttatcgatat ataagtgcat ttgctcatcg aaataatttt gagtaaaaat gctctgtctc
     2881 aaatactgat tgtaggaaat gggcagcagc ggcaattcac tgaaaggcgg aacgtttgtt
     2941 tcgcaccaca gcacattggt tttgtgttta ttgccttcca atgcccaatc gcataaagga
     3001 agaataacga ccggaaaatc gagccctttt gccttgtgaa tggtcataat gtggatagct
     3061 tcctgatcgg ccggcatggc tacaaattta ttgcagcccg cttcgtccca ccaagataga
     3121 aaatcgctaa tatcggaaaa acctttggct gtaaaattat ataccaaatc ctgaaaagct
     3181 tgaacaaatg gcgcctgatt ttcccattca cctattttga aaagggaaat aatttgttct
     3241 acgcggtcaa aaagcgaata atgacgaatt gcctgtagtg ctttttcttc ttcggaatca
     3301 aatagatggg agaatttatt ttcggtattt ttctttaatg cagcaatgtc ccgtaaggct
     3361 tcattctttg tttttcccaa tttatttaac gcatattcga ggcttacgat tgtcagttga
     3421 atgtcgtcgg aaggagatac aaaaagttta agcagtccga cgataaaacg taccgaagat
     3481 gctttcccta tgagcaatcc ttcatttccc aatatatcgt aacaataacc ttctttggct
     3541 gagggcgagt ttttgtagtt cattaaactt tcaatgacaa gggctgcgtc ttttttatcg
     3601 cgtaccaata tggcaatatc ggaagctttg taaccacgat cctgaaaatt ttcgagcatg
     3661 attggcaagc ggttcaaact ttcttcttgc cagttttttt tgttgttggg aataaattgg
     3721 atgcacacat gcccggctcc ggctttttct gaaactttct gttcggtgtc tgtataggca
     3781 tgttgaattt tgttggatag ttcggacaca aatggatatt tgtcggccgg caaatattcc
     3841 tgaatgttgg cattcagctg ttgttgcaga agctgagcag attgagaaaa aaaattattg
     3901 ttaaatttca cgatattcct gtcgctgcgc caattgacgt tgagtggttc ctggcgaatt
     3961 tgttgcggag taaaatcctt gaaaatttgt tcgtccaaca atttccaatc ggaatttctc
     4021 cagcggtaaa tactttgctt cacgtctccc acaaccatgt tgaaattatt ttctgccaaa
     4081 ctgtttcgca caagcgggag aaaatttttc cactgaagta ccgatgtgtc ctgaaattcg
     4141 tcaatcataa aatgctgcag cttgttgccg attttttcgt aaacgtaagg cgtatcgcta
     4201 ttgtcgataa tacgattgag caaaagattg gtgtcggaaa ttaaaaatgt gttttgttcg
     4261 gcagtcaact gattgatttc catagccaaa tcagaaagaa ttcccagttt gtaaatgtgc
     4321 ttcaatacta tgtcgacgga attgtagcgg ataaagggct cttgaaaaat ctcgttaatt
     4381 ttctcaaaac attttcccaa tccgcttata taggctgagt tgactttatt ttttacccaa
     4441 ttgggtgtgc ttttattggt acagttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnncgcg tcgttttcca tttcgaggtt gtaggctcca ttgataccca tctcacgtgc
     4621 aaaactgcga ataacctgct gaaagaaact gtcaattgtg ctgacagcaa atgaagagta
     4681 atcgtacaac agacttacga gtatttgttt ggctttgaga ttgacctctg cttcattgcg
     4741 accgtattct tccattaaac tttttcggta attgctgggt tgcccttgtg ccagcgcaaa
     4801 aagttccttc agaatacgtg atttcatttc ttcggtagct ttgttggtaa aggttaccgc
     4861 aagaatatgg cgatgaagat tttcaccttg atggcggaaa agcaaatgaa tgtaatcttt
     4921 tgtgagtcgg aaagtttttc ccgacccagc agaagcccga taaacattca acataaaaaa
     4981 tttttttaca gggtgatgaa aataaacgat ttttacttga aattgatttt tttgactttg
     5041 taacccattt tttggagtat ttctgcaatt ttcacacgga aatcgccttg tatgagtacc
     5101 attccgtgtt ctgccgtgcc tcctgcgctg catgaaactt ttagcgtttt tgcaagctcc
     5161 ttcagttcct tttcgtttcc ctcaaattcg gaaacaatag taaccggctt tccgttgcgt
     5221 ttttccattt gtactcgtaa aggttcggtt tgcacttttt tctccggcag ttcattttct
     5281 gtcggttcac tattatcttg tggcaaactg tctttcagtt gattgagttt ttctttccag
     5341 tccatgaggt aactataaat aagcggcaag ttaataattt ttttgtagaa agagcaatag
     5401 aaaaatgaaa tgaagaaaat attctttcag cttttcgcac atcttcttca aattcaaggt
     5461 agaagccaac aaaaaagcat tcatttaatg ctgtcttttt ataggaaaaa attctatttc
     5521 attcggaaat cttttgacgc atgtccgatg aattttagaa agcctgctct tgcgaggtac
     5581 tcgatcatca tgcatatcgg tacactcgaa gctctgttat ccgaataata gggctccaaa
     5641 tttttatcaa aataactgcg attaattttt ttaatccgca gattcttttc gtagtttatg
     5701 tcagtaaagt ttttctaccg gggaccaaat aaattccgtt attttttgca atttttctgt
     5761 agtttttttg gtattttata gcttaacttt actttgggaa gcaaaacttg caggtatccc
     5821 attttttttt aatatctttg catattgaaa atactctcac tataaaggga aaaaatgaaa
     5881 gaagttacaa aagtattgta tatttcccag gaaatttttc cttatctgcc tgcatcggaa
     5941 atggcaaata tgggaagata tcttcctcag gcagtacagg aaaaaggatt ggaagtacga
     6001 attttcatgc ccaaattcgg atctattaat gaacgaagaa atcaacttca cgaagtaatc
     6061 agactttcag gaatgaatat cattattgat gataccgacc atccgctgat catcaaggta
     6121 gcctctattc ctgctgcccg aatgcaggta tatttcatcg acaatgaaga ttattttcag
     6181 cggaaaaata cggtggccaa tgaaaataac gaagagtatg atgataatga cgagcgggct
     6241 atattttttg cgcgcggcgt gatggaaacg gtaaaaaaac tgcgctggtc gcccgatgta
     6301 atccattgtc agggttggat aacggctttg gtgcctttgt atgtaaaaaa aatttataac
     6361 gacgatccgt ttttcaagca ttcaagagtg gtttattcca ttttcgatga cgaatttaaa
     6421 aagcccctta acgatcgctt aatggacaag attttaatgg atggcattca gaagaaacat
     6481 ttggcttcca taaaaaatcc acctgtcgat taccattcac ttcttaaact tgctgccgat
     6541 tattccgatg gctttatcca atccagtccc aaagtcaatg aatcggtgtt gaagtatgtt
     6601 gccgacaaaa agattaagtt tttgccttat cagccggccg aagattattc cgatcaatat
     6661 attgattttt acaatcagct tttctaaagt ttacatatcg ataatcatat attcaatatg
     6721 catttcaaca aatttttcct gtttcttttt acactggctt ttgtttcgtg tacggatgga
     6781 ttgaccgaca taggcgtcag cataatgcct tcacaggata ttatcagtgt taacgccgat
     6841 gcatttgatt tgaacaccga aaatgttttt gttgatttta tttacacgcg tcaggattct
     6901 ttcttgttgg gaaattttta cgaccataaa tacggttcta cccgcgcaga tattctggca
     6961 caagtaaact gccctgtcga ttttactttt cccgataatg ctgttcccga ttcggctgta
     7021 atagctttgc gttatctcac ctggtttgga gataaatatt ctccaatgga aatcaaaatt
     7081 tatcaaatga ataaggctac tttttcctat tcggagcatt atccctccaa tatcaatccg
     7141 gatgattata ccgatcggtc tattcttttg ggaaaaagaa ttatcacgcc aagcgatact
     7201 tccggttcgc gttccgatgg gacacaagta cgtttcaagc tttccgatag ttttgttcaa
     7261 tggtttttcc ccgaaaatcg gacctacaac agtcaaaagg attttcttca gaagttcaaa
     7321 gggatgttta ttaccacaac ttttggcagc gcaaacattc tttatgtgaa tcaaatcgat
     7381 ttgtactatt actatcatta tacttatgta cgaaagactt ccgatggttc cgactcgacg
     7441 gttacagtca ataatgttat tacttttcct gccaatgccg aagttcgtca ggttaaccgt
     7501 attcagcatc ctgacagaaa tgaagtcgtt caatcgcgtc cagaggtgaa ttatgttgcc
     7561 tctccggcaa atatcaacac acgaatttcg ttgcctgtcg ataagatagc gaagcaaatg
     7621 aaaacaacca tccacaacaa atatctttcc gtgaacagtg cgttgttgaa agtagaggtt
     7681 accgaacagg atgaagcaac tattgccgtt cccctggtgc aaaacatgat gttgataaaa
     7741 gaatcggctt tggacagatt tttcaaaaac agcgaattgc cttccgatac ctgtgcattg
     7801 atagctcagt attcctatac aaaaaatgcc gataccaata gttacgatta ttattacact
     7861 ttcaaccttt ccaaattgat tgcgaatgaa ataaaaaagg ctgaaaccaa tggtacccaa
     7921 cttcccgaaa ctatggactt gttgctggtt cccgttaccg tgcaatataa caacagtggg
     7981 tactacacca gtgtgaaaca tcagatttta ctgagtggag taacgatcaa aagcgggaaa
     8041 aatacttctt ccccaatgaa aatcaatttg gtgtacagtg gtttttaaat caccgtaaat
     8101 tttatttttt tctgaaaaac agttggatgg gtgttccggt aaaattccac tgcgatctga
     8161 ttttattttc cagaaatcgc cgataaggtt ccttgatgta ttgaggcaaa ttggcaaaaa
     8221 agataaatgt gggaacttgc gtatcaggca gttgcgtaac atattttatc ttgatgtatt
     8281 tccctttcag tgccggtggc ggaaaattct gaatcaaagg aagtaaatat tcgttcaact
     8341 tggcagttgg aatttttttg tttttgtttt cgtaaacttt tactgccgat tcgagcacct
     8401 tgaaaattct ctgacgggtg agtgccgaaa taaaaataat cgggaaatcg gtaaaaggag
     8461 acatcctttc ccgaatatac gcttccattt ttttagtgga atgggtgtct ttgtcttcta
     8521 tcaaatccca tttgtttaca caaaccacta ttcccttttt gtttttctga ataatggaga
     8581 agatgttgag atcctgactt tccaaacctc tggttgcatc aatcagcagc atacagacgt
     8641 cgctgttttc aatagcgcgg atggaacgca cgatggaata gtattccaaa tcttcatcta
     8701 ctttggcttt tttgcgaatg ccggccgtat cgaccaaata aaaattgaat ccgaatttat
     8761 tgaaacgggt ataaatagaa tcccgcgtag tgcccggaat ttcggttaca atatttcttt
     8821 cctcgtccaa aaaagagttg agaagtgagg attttcctgc attgggtctt cctacaatgg
     8881 caaatcttgg cagatgctct tccgtttctt catcctcttg gggtttcaaa taacttacca
     8941 gtttgtcgag caaatctccg gttccaagcc cgttgatgga agaaaccaaa aacggatcac
     9001 ccatgcccag tttgtaaaat tccggtgcct gatactgcca ttcgaatgta tcggctttat
     9061 tggtaaccag aataaccggt ttgttggttt ttttgagaag ttgcgcaact tcaaagtcca
     9121 agtcggtaat gccgcactgt acatcaacca aaaaaagaat tacgtcggct tcgttgatgg
     9181 ccagttgaac ctgacgtttg atttcctgtt caaaggtatc ggaagagttg gttacccaac
     9241 cgccggtgtc gatcaccgaa aattcgtatc ccgaccattc gcttttgccg tactggcgat
     9301 cgcgcgtagt tccggctgtt tcgttcacaa tggctctccg cgtttgcgta agccggttaa
     9361 aaagagttga ttttccaaca tttggacgcc ctacaatggc tacaatattt cccataattt
     9421 tttatttttc atttgtaaat cttcaatcgg agtattccca tattccgatt gtgggtgaaa
     9481 atatttttta atttcgatgc aaagttaagt aaaacaattg actttgctgt ggtcgtgttt
     9541 ttttgggctc agaaaagcag ggtactgaag ccaatttttt taattgccaa aatagcggcg
     9601 taaaagaata ttcaacccct ttgtctgtta attgaactgc ttcatttgtg aaaccacctc
     9661 gttgtggatc aattccgcaa attcttccac gctcatgctc cctgcatcgc cttctccctg
     9721 tcggcgtacc gaaacgtgtg aagtttccat ttccttttct cccacgataa gcatgtaagg
     9781 aatacgtttc agttcgttgt cccgaatttt tcgaccaatt ttttcgtttc tttcatctac
     9841 ggtaacccga atggattttt ctttcagttt tttggcaata tcgaaagcat aatcgttgaa
     9901 tttttcactg atgggcagca ctactacctg atcgggggta agccacagcg gaaattttcc
     9961 tgccgtatgt tcaatcaaaa cggcaataaa tcgctccatc gaaccaaaag gtgctcggtg
    10021 aatcatcacc ggcctgtgtt tctggttgtc ttcaccggta tattccagct caaagcgttc
    10081 cggcaggtta tagtccacct gaatggtgcc aagctgccat tttctgccaa tggcatcttt
    10141 taccataaag tccaacttgg ggccgtaaaa agcggcttct ccctgaacaa ttttggcttt
    10201 cagccctttt tcttcacagg cttcaataat ggctttttcg gctttttccc aattttcgtc
    10261 cgtgccgata tatttttcct tattgtccgg atcgcgcagg gaaatctgcg cttcaaaatt
    10321 tttaaaatcg agtgcattga aaataatgaa aatgatgtcc atcactttca aaaattcatc
    10381 tttcacctga tcgggacggc agaaaatatg ggcatcatcc tgcgtaaaac ttctgacgcg
    10441 tgtcaatccg tgaagttcgc cgctttgttc gtagcggtaa accgtaccaa attctgcaaa
    10501 acgcagtggc aaatccttgt aggaacgcgg tttaaatttg taaatttcgc aatgatgcgg
    10561 gcagttcatc ggtttgagca aatattcttc tccttcttcg ggagtatgaa tgggctgaaa
    10621 agagtctttt ccgtatttgg cataatgacc ggaagttaca taaagttgct tgttgccaat
    10681 gtgaggagtg atgacttgtt gatattcaaa tcgctgttga attgttttga gtacattttc
    10741 aagcctgagg cgtaaatctg taccgcgagg caaccaaatg ggaagccctt tgccaaccat
    10801 ttcggaaaac atgaaca
//
LOCUS       DAOSIB010000072        10749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_6023_length_10749_cov_11.963344, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000072 DAOSIB010000000
VERSION     DAOSIB010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10749
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6023_length_10749_cov_11.963344"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1433
                     /locus_tag="PLD44_06900"
     CDS             162..1433
                     /locus_tag="PLD44_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOF99096.1"
                     /translation="MQPLAERLRPQTLDQFIGQKHLVGENAILRQMIESGHIASLILW
                     GPPGVGKTTLARIIARQLNCPFYTLSAINSGVKEVREVIDKAKNSRFFSQQSPILFID
                     EIHRFNKSQQDALLGAVENGTITLIGATTENPSFEVITPLLSRCQVYLLTPLDKNDLL
                     ELAQRAIDTDEELKKKKIHLDETEALLRFSGGDARKLLNIIELVVTAEPSETVHITND
                     IVTRRLQQNPMAYDKNGEMHYDIISAFIKSIRGSDPDAAIYWLARMIAGGEDPKFIAR
                     RLVISAAEDIGLANPNALLLANACFDALQKIGWPEGRIILAETTVYLAASQKSNSAYL
                     AIDDALNMVEKTGDLPVPLHLRNAPTKLMKELGYGKDYKYSHDYENHFVAEQFLPDQL
                     KNVSIWKKQDNAAEKKMAEWLEKLWKNRYQK"
     gene            1719..2228
                     /locus_tag="PLD44_06905"
     CDS             1719..2228
                     /locus_tag="PLD44_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOF99097.1"
                     /translation="MDTTIKLYHYNYRTLKTYLFATIFIVGNIIFPQLVHSIPNGGLI
                     FLPIYFFTLIGAYKYGFTVGLITAIISPLVNSWIFGMPPVGMLSIIITKSVLLAIGAA
                     YVASKSQKVSLVGLLLAILFYQLTGLMFEWFYTGSLILAYQDLRMGFPGLLLQWLGGY
                     ALLRSIQKL"
     gene            2234..2632
                     /locus_tag="PLD44_06910"
     CDS             2234..2632
                     /locus_tag="PLD44_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOF99098.1"
                     /translation="MEQKTFSEIMQRFADIRFDENFDLIVAIANGGIVPAGIINQKLK
                     TEIQILHINFRDDFQQPKYLTPQLLKPIDFEYTGKKILLVDDRIKTGATINVAKKLLE
                     GAQLIKTFAVNGNADYALYNESCFRFPWII"
     gene            2658..3596
                     /locus_tag="PLD44_06915"
     CDS             2658..3596
                     /locus_tag="PLD44_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOF99099.1"
                     /translation="MDRREFLKMLALSGAAFTIKQSKAMSLMTQHFSGKEINENYDLV
                     AVLGGTPVEMFRKGIAEFGGMEKFVKKGYKVVVKPNIGWDKTPEYAATTNPELVGEII
                     RQCFKAGAKEVTVFDHTCDDWRKCYASSGIEAAAKAAGAKVVPADLESYYESISLPRA
                     KNLKSAKVHKVLLESDVWINVPILKNHGGAKMTISMKNLMGIVWDRGFFHSHDLQQCI
                     ADICTMSKPAVLNVVDAYRLMKTNGPRGKSEADVVLAKGLFMSQDIVAVDTAATNFFN
                     QVRDMPLESVEHLAKAQELKVGTMNLNQLNIKRIKM"
     gene            3603..5129
                     /locus_tag="PLD44_06920"
     CDS             3603..5129
                     /locus_tag="PLD44_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOF99100.1"
                     /translation="MLKKLRVVISLLFFIPITLYFLDFRGVLPQQFHWLAKIQLVPAL
                     LSLNLIILTIIVGITFLFGRIYCSSVCPLGIFQDIVSWVSKQINKKKKFKFSTAKNIL
                     RWSVLGITVITFFLGFSFLTGFFDPYSIYGRMATHLFKPVYMEGNNLLAGIFNAAGFH
                     SLYKVQIYLLSLGTFLITLAMMLIIGYLSWKNGRLYCNTICPVGTTLGFLSKYSLFKI
                     RFDQEQCNSCGLCSMNCKSSCIDAKEKTIDYSRCVNCFNCMEACKRNGLKFATASALQ
                     RKNTENQTTTDIDNNKRRFLSTLAVSSLAVAVMPQEGIKINGFHKVKRETPVSPPGSK
                     SAENLMSACTSCHLCVSKCPSQIIRPSLFEYGLGGIMQPMLSFEKGFCNYDCTVCSEI
                     CPSGALSALTKEEKHKNQMGQVHFILKNCIVYKDETSCGACSEHCPTQAVNMVPYKNE
                     LTIPHTDTGICVGCGACEYACPAFPDKAIYVEGLKVHQQAAIKKEKSNHKIEIEDFGF
                     "
     gene            5165..5359
                     /gene="tatA"
                     /locus_tag="PLD44_06925"
     CDS             5165..5359
                     /gene="tatA"
                     /locus_tag="PLD44_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOF99101.1"
                     /translation="MTLTTILLAIGTPEILIIALVILLLFGGRKIPELMRGLGKGVSQ
                     FKKGMKDVEDEIKEDDNKKE"
     gene            5487..6293
                     /gene="tatC"
                     /locus_tag="PLD44_06930"
     CDS             5487..6293
                     /gene="tatC"
                     /locus_tag="PLD44_06930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOF99102.1"
                     /translation="MSLNNKANEQETTFWWHLEKLRWVILRTLAVVGLLMVLFFSNKD
                     FLFNKIILAPLNSDFYVYRWFNEIAKLLNFSDLTIDSFHLQLININLAGQFMAHLSIS
                     FAFALIASVPYLLFEIWRFIFPALYPHERKNIELIFFFSSLLFYLGAIVSYLIIFPLT
                     IRFLGTYQVSSLVPNQIALSSYISALYILVFSLGLTFEIPVLSYFLSRLGIITRQMLK
                     KGRGLALVIILIAAAVITPTTDPFTMMVVTVPLYLLYEVSILVCKKDTGQ"
     gene            6699..7076
                     /locus_tag="PLD44_06935"
     CDS             6699..7076
                     /locus_tag="PLD44_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOF99103.1"
                     /translation="MKKVIHTSDAPAAIGPYSQAVESSNILFISGQIPICPQEGKIIS
                     TTIDGQTEQVFSNIEAILKSAGYSFGQIVKTTVFLTDMSHFAAMNEIYKRFFTEDCPA
                     RSTIAVKELPQGALVEIETIAMK"
     gene            complement(7081..8037)
                     /gene="fmt"
                     /locus_tag="PLD44_06940"
     CDS             complement(7081..8037)
                     /gene="fmt"
                     /locus_tag="PLD44_06940"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOF99104.1"
                     /translation="MRKSKEELRIVFMGTPEFAVESLKILVEGGYNVVGVVTMPDKPA
                     GRGYKVQFSPVKQYALENNLLLLQPEKLKDEIFLENLQSLKADLQIVVAFRMLPEIVW
                     NMPPLGTFNLHGSLLPQYRGAAPINWAIINGEKETGVTTFFLTHEIDTGKIILQEKIP
                     ILPSDNAGTLHDKLMMLGASTVKKTVDLIISDAVQPIDQSELLKNTIELRSAPKIFRE
                     NCEIDVSWDVERAHNFVRGLSPYPGAWMNLVFPHTDEKTVLKIFQAEKEFSSHQLPLG
                     SLETDGKKFAKIALKDGFLVLKEVQAPNKKRMEIGEFLRGLR"
     gene            8293..10590
                     /locus_tag="PLD44_06945"
     CDS             8293..10590
                     /locus_tag="PLD44_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOF99105.1"
                     /translation="MKKLYLLSFAFILCFSLSSTAQKVGLVLSGGGAKGVAHIGVIKA
                     LEENGIPIDYIAGTSMGAIVGSMYAMGLTPEEMLNIIKSDDFRFWLTGQNEPEYVFYF
                     RNGNVKPTFMEISFRFNPHDTVRVKSNVLPTHIVPTHQLNYAFMALFSQATAASEQNF
                     DRLFVPFRCVASDIEEKVPVVFSKGSLGDAVRASMTFPLVFKPIVIDNKLLFDGGIYN
                     NFPVDVMRNDFNPDIMIGSVVAKNPQKPTEDNLLLQIENMVMSQTDYVIPKEEGFMLR
                     FDLKNFNMFDFTKADQLYQMGYDSTLQKIEEIKSAISRRISEEELNKNRQDFRDKFPE
                     LKFQNVYIEGIDSLQKLYVEREFHTGNEVFDLKSFKKSYFNLLSDNQISEITPAAVYN
                     KTSGLFDLHLKVKTEDHLKIMMGADISSMASNQAYIGLSYQNLKEYAQTAYVDAQFGR
                     MYNALGVGVRMDAAAKTDFYVKFGFVTHKFDYFDGISLFYNDNRVSYFNQQETYGKLS
                     IGFPLTKKGRIEFGLGGGYLADHYFQNKSLITSGVKNDKSSYSLGSVFVDAESNTLNR
                     PNYPTGGSYYAASLRLAGGNHNEYLNYPVVRELEGQELWLQFLGKYDRYINLSKNVTL
                     GTYGELAISNRKLLPNYTATMIQSPAFRPTLHSTTIFNEGYNANQYAAFGLKPIYSFN
                     NQLHFRTEAYCFVPYKTIYRLPDESAAYSKPFSDVEFISEAAMVLSFKKATLSAFVNY
                     YSAGSNHWNVGFNIGVLLFNKKFLQ"
BASE COUNT         3368 a         1993 c         2044 g         3344 t
ORIGIN      
        1 cgccgaactt ttttcaaaaa tatttctact cgaaactcgc aaccttgaat ctcataaatt
       61 gctaactcaa aaactataaa cttcatgaat ttttcaactg aaaaatcaaa aaaatcaaaa
      121 ttaagaacta aatttgtaat agtaattcaa aacaaatttt catgcagcct ttagcagaac
      181 gacttcgtcc acaaacactt gaccaattta tcggacaaaa acatttggtc ggcgaaaatg
      241 ccattttgcg tcagatgata gaatcggggc atattgcttc cttgatttta tggggcccgc
      301 ccggcgtggg aaagacaacg ttggcaagaa tcattgcccg acaactgaat tgtccctttt
      361 atacgctaag tgccatcaat tcaggagtga aagaagtcag agaagtaatc gacaaggcca
      421 agaacagtcg ttttttttcg caacaaagtc ctattttgtt tatcgacgaa attcatcgtt
      481 tcaacaaatc gcaacaggat gctttacttg gagccgtaga aaatggcact ataactttaa
      541 ttggtgctac cacagaaaat ccttcctttg aagtaattac gcctctcctc tcccgctgtc
      601 aggtttatct actcactcca ttggataaaa acgatttgtt ggaattggct caaagagcca
      661 ttgataccga tgaagaactg aaaaagaaaa aaattcattt ggatgaaacg gaagctttgc
      721 tgcgtttttc gggaggtgat gcccgcaaat tactgaatat catcgaactg gttgttactg
      781 ccgaaccttc cgaaacggtt cacattacca atgatattgt tacccgccga ctgcagcaaa
      841 acccaatggc ttacgataaa aatggagaaa tgcattacga tattatttct gcgtttatca
      901 aatccattcg tggcagcgac cctgatgccg ccatttattg gcttgcccga atgatagcgg
      961 gcggtgaaga tccgaaattt attgctcgaa gattggtcat ttctgctgct gaagatattg
     1021 ggcttgccaa tcccaatgct ttattgctgg caaacgcctg ttttgatgca ctgcaaaaaa
     1081 taggatggcc cgaaggaagg attattctcg ccgaaactac agtttatctg gcagcttctc
     1141 aaaaaagcaa ttcggcctat ctggctattg acgatgcttt gaatatggta gaaaaaaccg
     1201 gagatctgcc agttccactc catttaagaa atgcacctac caaactcatg aaagaacttg
     1261 gttacggaaa agattacaaa tattcacacg attacgaaaa tcattttgta gcagagcagt
     1321 ttcttcccga ccagcttaaa aatgtcagca tttggaaaaa acaggataat gctgcagaga
     1381 aaaaaatggc cgaatggttg gaaaagcttt ggaaaaacag ataccaaaaa taaaaaaatt
     1441 tctttctctc aagtaaaaaa gcaaatcctc tcaaaatcat tcaccattaa aaaaaccgga
     1501 gattttattt ttagtgcgac gtagccagga ggatacgccg aacaagacca aaccaccgat
     1561 tacaaatcgg cgggagcgag cgaataacat tataattttc attttcaatt taaaatgact
     1621 gtaaaatttg cttaattttg gagaatgatt tgttttttat ttatattttt gtcttatgct
     1681 aagattgaaa attaattctt taataatgat gatttgctat ggataccaca attaaacttt
     1741 atcactacaa ttatcgaaca ttaaaaacat atttatttgc aactattttt attgtcggga
     1801 atataatttt tccccagttg gtacacagca ttcccaatgg cggccttatt ttcctgccca
     1861 tttacttttt tactctaata ggagcttaca aatacggctt tactgttgga cttattacgg
     1921 caataatttc gcctttggtc aacagttgga tttttggaat gccaccggtt ggcatgttgt
     1981 ctatcattat tactaaatct gttttattgg caattggagc cgcctatgtt gcttcaaaaa
     2041 gtcaaaaagt gagtcttgtt ggattgttgt tggcaattct gttttatcag ttaaccgggc
     2101 tgatgtttga atggttctac accggcagcc ttattcttgc ctatcaggat ttgagaatgg
     2161 gatttccggg attgcttctg cagtggttgg gcggttatgc cttgttgaga tccattcaaa
     2221 agctgtaatt tgaatggagc agaaaacttt ttcggaaatc atgcaacgtt ttgccgatat
     2281 ccgatttgat gaaaatttcg acctgattgt tgctattgcc aacggtggaa ttgtccctgc
     2341 aggtattatc aatcagaagt taaaaacaga aatccaaatt ttgcacatca attttcgtga
     2401 tgactttcaa caaccaaaat atttaactcc ccaactatta aaacccattg attttgaata
     2461 tacggggaaa aaaattttgt tggtggacga tcgaattaaa accggtgcaa caattaacgt
     2521 tgcaaaaaag ctgctggaag gagcacaact tatcaaaacc tttgccgtta acgggaatgc
     2581 agattacgct ctttacaacg aaagctgttt ccgttttccc tggataattt gaaatttgtt
     2641 tttaaacaaa caacactatg gacagaagag aatttttaaa aatgcttgca ctcagtggag
     2701 ctgcttttac cataaagcaa agcaaagcca tgtcactgat gactcagcat ttcagcggaa
     2761 aagaaataaa cgaaaattac gatcttgttg ccgtgttggg aggaacgccg gttgaaatgt
     2821 tccgaaaagg aattgccgaa tttggcggaa tggaaaaatt tgtcaaaaaa ggatataagg
     2881 ttgttgtaaa accaaatatt ggctgggata aaactccaga atatgctgct acaacgaatc
     2941 ctgaattggt aggagaaatt attcggcaat gtttcaaggc cggcgccaaa gaagttaccg
     3001 tttttgatca tacttgcgac gactggagaa aatgctatgc cagcagcgga attgaagcag
     3061 cagccaaagc tgcaggagcg aaggtagttc cagccgattt ggaatcctat tatgaatcca
     3121 tttcactccc tcgtgccaaa aatctgaaat cggccaaagt tcacaaagtt cttttggaat
     3181 cggatgtgtg gattaatgtt cccattctga aaaatcatgg cggggcaaaa atgaccattt
     3241 ccatgaaaaa cctcatggga attgtttggg acagaggttt cttccacagc cacgatttac
     3301 aacaatgcat tgccgatatt tgtaccatga gtaaaccggc ggttttgaat gtggttgatg
     3361 cctaccgact gatgaaaacc aatggtccac gaggaaaatc cgaagcagac gtcgtgcttg
     3421 caaaaggatt gttcatgtcg caggatattg tagctgttga tacggctgcc accaattttt
     3481 tcaatcaggt gagagacatg ccactggaaa gcgtcgaaca tttggcaaag gctcaggaat
     3541 tgaaagttgg aaccatgaac ctcaatcagt taaatataaa acggattaaa atgtaaacag
     3601 agatgttgaa gaaattaaga gtcgtcattt ctctcctgtt tttcatcccg attactttat
     3661 attttcttga ttttcgaggt gttttgccac aacagtttca ttggctggcg aaaattcagc
     3721 tggtaccggc attgctctcg cttaatctca tcattctgac gataatagtc ggaattacct
     3781 tcctttttgg tcggatttat tgctcatctg tctgtccact gggcattttt caggatattg
     3841 tttcatgggt ttccaaacag atcaataaaa agaaaaaatt caaattcagt acagcaaaaa
     3901 acattctccg ttggtcagtt ttgggaatta cagttattac tttttttctg ggtttttctt
     3961 ttctaaccgg ttttttcgac ccttacagca tttatggacg tatggccacc catttgttta
     4021 aaccggtgta tatggaagga aacaacttgc tggcagggat tttcaatgca gcaggttttc
     4081 attctctcta caaagttcag atttacctgc tcagcctcgg cacttttctg attactttgg
     4141 ctatgatgct gataattggt tatttgtcat ggaaaaatgg acgtctttac tgcaatacca
     4201 tttgtccggt tggaaccaca ttgggatttc tcagcaaata ttcccttttt aaaatcagat
     4261 tcgatcaaga acagtgcaat tcctgcggac tttgcagcat gaactgcaaa tcttcctgta
     4321 tcgatgcaaa agaaaaaaca atcgattact cgcgttgcgt aaattgcttc aattgtatgg
     4381 aagcctgcaa acgaaacggt ctaaaatttg ctactgcatc tgctttgcaa agaaaaaata
     4441 cagagaatca gaccacaacg gatattgata ataacaaacg acgttttctg agcacactgg
     4501 ctgtttcgtc tttagccgtt gctgtaatgc ctcaggaagg aatcaaaata aatggttttc
     4561 acaaggtaaa aagagaaact cctgttagtc ctcccggatc aaaaagtgcc gaaaatttaa
     4621 tgagtgcctg cacatcgtgt catctttgcg tcagcaagtg cccatcacaa atcattcggc
     4681 catcactttt tgaatatggt ttgggaggca tcatgcagcc aatgctttct ttcgaaaaag
     4741 gattttgtaa ttatgattgt actgtttgct ccgaaatttg ccccagtgga gctctctcag
     4801 ctttgaccaa agaagaaaag cacaaaaatc agatgggaca ggtgcatttt attttaaaaa
     4861 actgcattgt atataaagat gaaacaagtt gtggcgcctg ttcggaacat tgtccgactc
     4921 aagcggtaaa catggttccc tacaaaaatg aattgaccat tccacatacc gataccggta
     4981 tttgcgttgg ttgcggcgct tgtgaatatg cttgtcccgc ctttcccgac aaagcaattt
     5041 atgtggaagg actcaaagtt catcagcaag ctgccataaa aaaagaaaaa tcaaaccaca
     5101 aaattgaaat agaagatttt ggtttttaag aataaaattt ttgaactatt aatttattat
     5161 cgacatgaca cttacaacaa ttttattagc tatcggaaca cccgaaattt taatcattgc
     5221 cttagttatt ctactgcttt ttggaggaag aaaaattcca gaattgatgc gcggattggg
     5281 aaaaggagtc agccagttta aaaaaggaat gaaagatgta gaagatgaaa ttaaggaaga
     5341 tgacaacaaa aaggaatagt tatcttatat ttcaagttat tcattctttt cccaaacaat
     5401 ttttttgagt aaaaatacca aaattaatat atggatagaa ctgatttgca aatgacatcc
     5461 atataacata acgataacaa ttcggtatgt cgttaaacaa taaggcaaat gaacaggaaa
     5521 ctactttctg gtggcatttg gaaaaacttc gctgggtcat tttacgaaca ttggcagttg
     5581 taggactgtt aatggtgtta ttcttttcca acaaagattt tctgtttaac aaaattattt
     5641 tagcaccctt aaattctgat ttttatgtct atcgctggtt taacgaaatt gcgaagttgt
     5701 taaatttcag cgatttgaca atagacagtt ttcatcttca gcttatcaat atcaatctgg
     5761 cagggcaatt catggcccat ttgtctattt cgtttgcttt tgccctcatt gcctcggttc
     5821 catacctgct attcgaaatt tggcgtttta tcttccctgc tttgtatccg catgaacgaa
     5881 aaaatataga attaattttc tttttttctt ctttgttgtt ttatctgggt gcaatcgtca
     5941 gttatctgat tatttttccg ttgaccatcc gatttttggg aacctatcag gtttcttcgc
     6001 tggtacccaa tcagattgct ctctcttctt acatcagcgc cttgtatatt ctcgtctttt
     6061 cattgggact tacttttgaa ataccggttc tctcgtactt tttatctcga ttgggaataa
     6121 tcactcggca aatgttgaaa aaaggacgag gacttgcact tgtgattatt ttaatagctg
     6181 ccgcagttat tactcctaca accgatcctt ttaccatgat ggttgtaact gttccgcttt
     6241 atttactcta tgaggtcagt attttggttt gtaaaaaaga cacgggacaa taacgtaata
     6301 gtttcggagc tgataacgtt tcaaatactg taaagaaatg catagataca ttagtcgaat
     6361 tgacaaccga tgatgatact cctttttaaa aactatcaaa acatgtcaaa tcgcattaag
     6421 tacaaattgt aaaggcaaca aaatctgcaa aagatattga ttcgggttac atattaattt
     6481 tggcaaggtt aagttgttgg tgttggatgt attcacaact tactaacgaa aattttggta
     6541 atcaatataa atgagttctg ttataaattc aatagaagat attttaaaac aacagaccaa
     6601 gaaaaaatta atttcttaac aataaatgaa tttttattgc gaaatttgta aaaacaaaaa
     6661 gttgcattcc ttacttgaat gctctaacac tactaattat gaaaaaagtt attcatacct
     6721 ctgatgcgcc cgcagctatt ggaccttaca gccaagcagt agaaagcagt aacattcttt
     6781 ttatttcggg tcaaattcca atttgcccac aagaaggaaa aattatttca actactattg
     6841 acggacaaac cgagcaggtt ttttccaata tcgaagccat tttgaaatcg gcaggatatt
     6901 cgttcgggca aatagttaag actacggttt tcctgaccga tatgagtcat tttgcagcta
     6961 tgaacgaaat ttacaaaaga ttttttacgg aagattgtcc ggcccgctca acgattgccg
     7021 ttaaggaact tcctcaggga gctttggttg aaatagaaac gatagccatg aaataaattt
     7081 ttatcgcagg ccgcgaagaa attcgccaat ttccattcgt tttttgttgg gggcctgtac
     7141 ttcttttaac accagaaaac catctttcaa cgcaatttta gcaaactttt tcccatcggt
     7201 ttccaaactt cccaaaggca actgatgaga agaaaattct ttctctgcct gaaaaatttt
     7261 caaaaccgtt ttttcgtctg tatgcggaaa aactaagttc atccatgctc caggatacgg
     7321 agagagtccg cgtacaaaat tatgcgctcg ttcaacatcc cacgaaacgt cgatttcgca
     7381 attttccctg aaaattttgg gtgccgatct caactcaatc gtatttttca acaattccga
     7441 ctgatcaatg ggttgcactg catcagaaat tatcagatca acagtttttt taacggtgga
     7501 agccccaagc atcatcaact tgtcatgtaa agtgccggca ttatcggaag gaagaatagg
     7561 aattttttcc tgaagaataa tttttcccgt atcaatttcg tgggtgagaa aaaaagtcgt
     7621 taccccggtt tccttttcgc cattgataat agcccagttg atgggtgcgg cacctcgata
     7681 ttgcggcaac aaggaaccat gaagattaaa agttcccaat ggaggcatgt tccacactat
     7741 ctccggcaac atgcggaaag caacaactat ttgcaaatct gctttcagag attgcagatt
     7801 ttccagaaaa atttcatctt tgagtttttc cggctgaaga agtaacagat tattttcaag
     7861 tgcatactgt tttacaggcg aaaactgaac cttgtatcca cgtcctgcag gcttgtcggg
     7921 catagtaact actcctacga cattgtatcc gccttcgaca agaattttta aactttcaac
     7981 cgcaaactcg ggtgtcccca taaaaacaat acgaagctct tcctttgatt ttctcatgtc
     8041 atttttttaa aatattgcag caatttttaa ttcggtctca acacaaaaat aataaaaaaa
     8101 ttttcatgct acggtcatca aaaaataaaa gcattaacaa cgtggagaaa ttttacagtt
     8161 tatcttcaaa aaatctaatt ttttcactct tttcgaaaaa aaagggtttt aatagggaat
     8221 ccaaattttt aagcaaaaaa ttatcattaa ctcaattttt tttgtaactt tatcctgtaa
     8281 atctcgattt taatgaagaa attatattta ttatccttcg catttatttt atgtttttct
     8341 ttaagttcta ccgctcaaaa agttgggtta gtattgagtg gtggcggagc caaaggcgtt
     8401 gcccatattg gagttattaa agctttggaa gaaaacggta ttccaatcga ttacatagca
     8461 ggaacttcta tgggggcgat agttggcagc atgtatgcaa tgggacttac accagaggaa
     8521 atgttgaata ttatcaagtc cgacgatttt agattctggc ttaccggaca gaatgaacct
     8581 gaatatgttt tctattttcg taatggtaat gtaaagccaa catttatgga aatcagtttt
     8641 cgtttcaatc cacatgatac agtgagggtt aaaagcaatg tcctccctac gcatattgtt
     8701 cctacccatc aactcaatta tgcttttatg gctttatttt cacaggcaac agccgcttcc
     8761 gaacagaatt tcgatcgact ttttgtcccg tttcgttgtg tagcttccga cattgaggaa
     8821 aaagttccgg tagttttcag caagggctca ttgggcgatg cagtaagggc ttccatgact
     8881 tttccattgg tttttaaacc catcgtaata gacaacaaat tgctttttga tggaggtatt
     8941 tacaataatt tccctgtaga tgtgatgcgg aatgacttta atcccgatat tatgattggg
     9001 agcgttgtgg caaaaaatcc gcaaaaaccc actgaagaca atcttctttt acagattgaa
     9061 aatatggtga tgtctcaaac cgattatgtt attccaaaag aagaaggctt tatgcttcgt
     9121 ttcgatttaa agaattttaa catgtttgat tttacaaaag ctgatcagct ttatcaaatg
     9181 ggttacgatt ccacacttca gaaaattgag gagataaaat cggccatttc aagaagaatt
     9241 tcagaagaag aactaaataa aaaccgacag gactttcggg ataaatttcc tgaacttaaa
     9301 ttccaaaatg tttatataga aggaatagat tctttgcaaa aactttatgt ggaacgcgaa
     9361 tttcatactg ggaatgaagt tttcgatttg aaaagtttca aaaaaagtta tttcaatctc
     9421 ctttccgata atcagatttc ggaaatcacg cctgcagcgg tgtacaataa aacttccggt
     9481 ttatttgatt tacatttgaa agtcaaaacc gaagatcatt tgaagattat gatgggagcc
     9541 gacatttcat ctatggcaag caatcaggca tatatcggac tttcatatca aaatttgaag
     9601 gaatatgcgc aaacggctta tgtggatgct cagtttggta gaatgtacaa tgcgctggga
     9661 gttggtgtta gaatggatgc ggcagccaaa accgattttt atgtgaagtt tggctttgta
     9721 acgcataagt ttgattattt tgatggaatc agcttgtttt ataacgataa tcgcgtgtcg
     9781 tatttcaatc agcaggaaac atacggtaaa ttgagcattg gatttcctct gacaaaaaaa
     9841 ggtagaatag aatttgggct gggaggaggt tatttagccg atcattattt tcagaacaaa
     9901 agcttgatta cttcgggagt aaaaaacgac aaaagtagtt attcattggg aagcgttttt
     9961 gttgatgccg aaagtaacac gctaaatcgt ccaaattatc ctactggcgg atcttattat
    10021 gctgcttcgt tacgtttggc cggcggtaat cacaatgaat atcttaatta tcctgttgtg
    10081 agggaactcg aagggcaaga gttgtggctg cagtttcttg gaaaatatga tcgatatatc
    10141 aacctttcga aaaatgttac attgggaact tatggggaac tggccatttc taaccgaaaa
    10201 ttgctaccca attatacggc tactatgatt cagtcgcctg cattccgtcc gacgttgcac
    10261 agtactacca ttttcaacga gggttacaac gccaatcaat acgcagcatt cggactgaaa
    10321 cccatttaca gtttcaacaa tcaattgcat tttaggaccg aagcctattg ttttgttcca
    10381 tataaaacca tttatcgtct tcctgatgaa tcggcagcat attccaaacc tttttccgat
    10441 gtagaattta tttcggaagc tgctatggtt ttaagcttca aaaaagcaac actttctgca
    10501 ttcgtgaatt attacagcgc tggttcgaat cattggaatg taggttttaa tattggcgtt
    10561 cttcttttta acaaaaaatt tctgcaataa aatctgagtg taacgatttg taggcaaaga
    10621 gcaaaaaatt aatttcatcc ttccgaaaaa atgatgaaat atctttttag aagtagatta
    10681 aaataatgga aatacctatt ccaataggga agagaaagta aatttatttt tcgggacaaa
    10741 tataaaaaa
//
LOCUS       DAOSIB010000073        10737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_6034_length_10737_cov_14.029114, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000073 DAOSIB010000000
VERSION     DAOSIB010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10737
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6034_length_10737_cov_14.029114"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..1699
                     /locus_tag="PLD44_06950"
     CDS             236..1699
                     /locus_tag="PLD44_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOF99106.1"
                     /translation="MNSNDIRQLQPRQLWENFYSLTQIPRPSGKKEAIGQFLENFGKS
                     LGLETLRDEIGNVLIRKPATFGMEKRKPVILQAHMDMVPQKNSNVSHNFDTDPIQAVV
                     DGDWVRAQETTLGADNGIGLAAALTVLQSDNIAHPALEVLVTVDEETGMHGAFALKPN
                     FLKGEILLNLDSEDEGELYIGCAGGLDADITFRYQEVPVPESDVALKISLTGLKGGHS
                     GVDIHLQRANANKLLFRFLKKAVAEYEARLAWVEGGNLRNAIPREAFALITIPADGVD
                     DLIELLKDFENLFVEENQFVEDNIRLQVEKVKLPTGLMPEEIQDDLINGVTACPNGVF
                     RFIPEVSDVVETSNNLAVVKSDGSTIRLRCLLRSSVESRKEELASMVESVFSLAGAHI
                     DFSGGYPGWKPNLDSPILKVMTEVYEKQFGSKPKIKIIHAGLECGIIGMHYPDMDMIS
                     FGPTIRYPHSPDEKVNIASVEKFWDFLLATLQNIPEK"
     gene            1820..3013
                     /locus_tag="PLD44_06955"
     CDS             1820..3013
                     /locus_tag="PLD44_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOF99107.1"
                     /translation="MMKKLTIISILILSILQLYAQKDTTAVDSFRFTTIKENKITPIK
                     DQASSGTCWSFSGLAFIESELLRMGKPEVDLSEMFIVHHTYSDKADKYVRMNGTINFA
                     AGGSFFDDLYALKNYGIVPETIMNGLNYGEEKHRHGELDALTEAYVKVIVSNPNKKLS
                     TAWKQGFDGILDAYLGKCSEKFIWEGREYTPLTYFQSLGINPDDYVSLTSFTHHPFYT
                     RFAIEIPDNWRWGESYNLPLDEFMSVFDYAVNQGYTIAWASDVSEKGFTRNGIAVNPE
                     TKLEKLPGSDQAKWLNISPKEMEEKIYKIQGPQPEVQVTQESRQRGFDNYQTTDDHGM
                     QIFGIAKDQNGTKYYMVKNSWGTDNKYKGIWYASESFVKDKTINILVHKNAIPKEIKT
                     KLGIK"
     gene            3414..3623
                     /locus_tag="PLD44_06960"
     CDS             3414..3623
                     /locus_tag="PLD44_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99108.1"
                     /translation="MPKSYFRIKEKYRDIAEVVRSRILLVSGFILELDANQRKQVDQL
                     LTYWKQTQKAWYQVLYDQNNKNQVN"
     gene            complement(3959..4711)
                     /locus_tag="PLD44_06965"
     CDS             complement(3959..4711)
                     /locus_tag="PLD44_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOF99109.1"
                     /translation="MKNLYKQILINRECNKKMLAVLLDPDRCQGKLLSSTIAALKLSA
                     PDFVFVGGSYVNSPIDSFIELLQEEVQIPVVLFPGNASQFSPKADALLFLSLISGRNP
                     EFLIGQHVTSALAIKQSGIEVIPAGYILIDGGKVSAVEYMSHTRSIPREKKEIVLATA
                     VAGELSGQHVVYLEAGSGAIQPVPKELINHVTSHLSIPMIVGGGMKTTQHLNDCFDAG
                     ADLAVVGNAVEQNPDLIVEFTNFVKNYNTIHR"
     gene            complement(4763..5416)
                     /locus_tag="PLD44_06970"
     CDS             complement(4763..5416)
                     /locus_tag="PLD44_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOF99110.1"
                     /translation="METMIHKKIETSGAKILVWQSTETFDELVTFFDKNETEELNTLH
                     SEKRKIEYLSVRLALKTLLNREIKICYNDDGKPFLSDYFAEIGISHSGQWIAVITHPT
                     CKVGIDIERFQPKISAISKRFLSKEELEKILEKDENRLKKLTLLWSAKESLFKVIGKE
                     AVDFSRQLCILPFEMGKTGKIKALHIPRNKLYELNYELSEEYAMVYLINKNVEPNKL"
     gene            complement(5404..6009)
                     /gene="gldD"
                     /locus_tag="PLD44_06975"
     CDS             complement(5404..6009)
                     /gene="gldD"
                     /locus_tag="PLD44_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HOF99111.1"
                     /translation="METKKIKYLQTIFYKTTLLIVITASLFSCGKNYVPRPYGYFRVD
                     LPPHNYRYLDTLQLPYRFEISSYAKVEKREAEGEKCWIDIKYPFLNAAIYCSYKPIHG
                     NLKELLEDTRQMAYKHSIRADAINEKLYQHPEKKVYGILYDLKGNTASSVQFFVTDSI
                     HHFFRGSLYFDNVPNKDSIAPMSDYIRQDIIRLMESFEWKQ"
     gene            complement(6070..7377)
                     /gene="gldE"
                     /locus_tag="PLD44_06980"
     CDS             complement(6070..7377)
                     /gene="gldE"
                     /locus_tag="PLD44_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HOF99112.1"
                     /translation="MIAFHSFSVSAAIALLLSILLLMCSAIISGSEVAFFSLTPQMKN
                     EVEESNRKNAKNLMHLIGNPQQLLATILVGNNFVNVAIIMLLTYFTGSVFDFSTFPLL
                     GFIFETVIITFVLLLFGEIIPKVYASQYPEKMALLFTPFILVMQKVFSRVSKLLISST
                     AFLTRQIEKYKKAEISIDELSHALELTTDEQDDDKEMLEGIIKFGNIRVAEIMTSRVD
                     MIAVDTKTNFKDLLKIIIQTGYSRIPVYSENRDNIKGILYSKDLLPHLDKPANFRWQS
                     LIRQAYYVPETKKIDDLLSEFQKNKVHLALVVDEYGGISGLVTLEDILEEIVGDISDE
                     YDDEEKLYTKVDNHTYIFEAKILLNDFFKVTGINEEEFSKVTEEVETLAGLVLELKGE
                     MPAKNEMIEYGRYVFEILSVDNRRIRKIKLHMKDNFDRNKEEN"
     gene            7739..8848
                     /locus_tag="PLD44_06985"
     CDS             7739..8848
                     /locus_tag="PLD44_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOF99113.1"
                     /translation="MENKFVTLAIRTYKRAQMIKTTLEENGIETRIESVNLEHPEMSV
                     GVRIRIKENDLPRALKIVEEMEIAWEEKGETLKRAEKMILLPIDFSDQLPKIIDFGFY
                     FAESLNAKVELLYVYYSPPFTISSVNNEVNTYTLSDSELLRQIISNANREVTSMTNTI
                     NERIAKGELPNVPFHFELREGVPEDQIVDFCKKYQPALVIMGTRGKKISSELIGSVTA
                     EVIESCVSPVLALPVDMKINTPSEIKRIAFLTNFEQKDLIAIDNTISLFNWNSLEMFF
                     IHAAQKKEVWDEVMLAGIKEYFSKHYPKIITHYELIDSTETMKQIDDYLVKHDINLLA
                     FNSPRRNLIRRLFNPGLAFKMVLHSDTPLFVTHIS"
     gene            complement(9283..9567)
                     /locus_tag="PLD44_06990"
     CDS             complement(9283..9567)
                     /locus_tag="PLD44_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99114.1"
                     /translation="MKQVNSIYRFQLQIGAIKTTSLIEGFIIGVWCGDNQFLHIEFTR
                     NDAAQGKILVEKIHRLKTFINASSPSSHRLPTNFEEEEVYKDYCYSYSAL"
     gene            complement(9751..10281)
                     /locus_tag="PLD44_06995"
     CDS             complement(9751..10281)
                     /locus_tag="PLD44_06995"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOF99115.1"
                     /translation="MINTTAFHSACFGFASTPFGKCCVAKDDRGIFSLTFPFDEEFAI
                     NELFGKFPDKKFYHDDKEIGIMAQLIFQRNICPALNFYGSDFQKTVWEALLKIPFGGR
                     VSYEEIAISIGMPKAVRSVAHAIACNPIAFLVPCHRVILKNGSIGEFRWGRERKVAML
                     EWEEKILNLEKFQEIE"
     gene            10380..10737
                     /locus_tag="PLD44_07000"
                     /pseudo
     CDS             10380..10737
                     /locus_tag="PLD44_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611509.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4372 domain-containing protein"
BASE COUNT         3462 a         2010 c         1997 g         3268 t
ORIGIN      
        1 aagtctcttt tgaaaaaaca atatgcttta ttcagaaggg ttacaagtat aaatatcttc
       61 taaatgaaag tttttctgat aatggtgatt ttcaacttct ttttttacca atctgtagaa
      121 tattttgtaa aaaatctttt caactgttca tttatctgaa aaatttgagc atatttgtat
      181 tctaaaaatt ggaataaata ttcagaattt acaaaaccct aataataaac atattatgaa
      241 tagcaatgat attcgtcagc ttcaaccgcg gcaattgtgg gaaaattttt acagtctcac
      301 acagattccg cgtccttctg ggaaaaagga agccatcggt caatttctcg aaaattttgg
      361 aaaatcactc gggttggaaa cattgcggga tgaaatcggc aatgtattga ttcggaaacc
      421 ggcgactttc ggcatggaaa aaagaaaacc cgtcattctg caggcacaca tggacatggt
      481 tccacaaaaa aacagcaatg tttcacataa ttttgatacc gatccaattc aagcggttgt
      541 tgatggcgat tgggtaagag ctcaagaaac tacgctggga gccgacaacg gtatcgggct
      601 ggctgcagca cttactgttt tgcaatcgga caatattgct catccggcgc tggaagtgct
      661 ggttacagtg gacgaagaaa ccggcatgca tggtgctttt gctttgaaac caaattttct
      721 gaagggagaa attctcctca atctcgattc ggaagatgaa ggagaattgt atatcggttg
      781 cgcgggcgga ttggatgccg acattacgtt ccgctatcag gaagttcctg ttcccgaaag
      841 cgatgtcgct ttaaaaattt ctctgacggg gctgaaaggt ggacattctg gtgtggatat
      901 tcacttgcaa cgagccaatg ccaacaaatt gctgtttcgt ttcctgaaaa aagccgtagc
      961 cgaatacgaa gctcgcctgg cctgggtgga aggtggaaat ttgcgtaatg ccattccacg
     1021 cgaagctttt gctctcatta ctattcctgc cgacggtgtg gacgatttaa ttgaactgtt
     1081 aaaagatttt gaaaatttgt ttgtagaaga aaaccaattt gtggaagaca atattcgatt
     1141 gcaagtggag aaggtaaaat tgcccaccgg cttgatgccc gaagaaattc aggatgacct
     1201 gattaatggt gtaacagctt gccccaatgg tgttttccga ttcatccccg aagtttccga
     1261 tgtagtggaa acgtccaaca acctggcagt ggtaaaatct gatggctcaa ccattcggtt
     1321 acgttgtctg cttagaagtt ccgtagaatc gagaaaggaa gagttagctt caatggtaga
     1381 aagcgtcttt tcactggcag gtgcgcacat tgatttttcc ggcggttacc ccggctggaa
     1441 acccaatctg gactcgccaa ttttaaaggt aatgaccgaa gtttacgaaa aacaattcgg
     1501 ttcgaaaccg aaaatcaaaa tcattcatgc cggtttggaa tgtggcatta ttggcatgca
     1561 ttatcccgat atggatatga tttctttcgg accaaccatt cgatacccgc attcgcccga
     1621 tgaaaaagtc aatattgctt ccgtcgaaaa attctgggac tttttgcttg caacactgca
     1681 aaatattccg gaaaaataaa tttcagaaaa aaaataaaaa actcatttcg ataaaacata
     1741 tgaaaccata cacaaacacg aatgaatata atattcagac atgtgggtta catcagacaa
     1801 ttaaaaaata aattatattc tgatgaaaaa attgacaata atttctatac tgattttaag
     1861 cattttacaa ctttacgccc aaaaagatac gaccgcggtg gacagttttc gcttcacgac
     1921 catcaaagaa aataaaatca cgccaatcaa agaccaggca agctccggaa cttgctggag
     1981 tttttccggc ttggctttta tagaatcgga attgttgaga atggggaaac ctgaagttga
     2041 tttgtccgaa atgtttattg tgcatcatac ctattccgac aaagccgaca aatatgtacg
     2101 catgaacgga accattaact ttgctgccgg aggttccttt ttcgacgatt tgtatgcgtt
     2161 gaaaaattat ggcattgttc ctgaaaccat tatgaacggg ctaaactatg gcgaagaaaa
     2221 gcacaggcac ggtgaattgg atgctctgac ggaagcttat gtaaaggtaa tcgtttccaa
     2281 cccgaacaaa aaattatcaa cagcatggaa gcagggcttt gatggaattc tggatgctta
     2341 tttgggaaaa tgttccgaga aattcatctg ggaaggcaga gaatatacac cgctcactta
     2401 ttttcaatca ttgggaatca atcccgacga ctatgtttcg cttacttcat ttacgcatca
     2461 tcctttttac accagatttg ccatcgaaat tcccgataat tggcgctggg gtgaatcgta
     2521 taacttgccg ctggatgaat tcatgtcggt tttcgattat gcagtcaatc aaggttatac
     2581 tatagcctgg gcatcggatg tcagcgaaaa agggttcacc cggaatggca tcgccgttaa
     2641 tcccgaaaca aaactggaaa agctgcccgg ttcggatcag gcaaaatggc tgaatatttc
     2701 acccaaagaa atggaagaaa aaatttacaa aattcaggga cctcagcccg aagtgcaggt
     2761 aacacaggaa agccgtcagc gtggattcga taattatcag actaccgacg atcacgggat
     2821 gcagattttt ggcatcgcca aagatcagaa cggaacaaaa tattacatgg taaaaaattc
     2881 gtggggaacc gataacaaat acaaaggtat ctggtatgcc agcgaatcat ttgtaaaaga
     2941 caaaaccatt aatattttgg ttcacaagaa tgctattcca aaggaaatca aaactaaact
     3001 gggcatcaag taaatcgacg gatttatcca gacattttgc tgttactgag tttgctgcaa
     3061 aataagattt gagaacaaat ggccttgatt gatgaaacaa atcaatggtc gtggttacta
     3121 agcgctcaaa gagtttttac gaaattcttt gggcgaaatt ctttttattt ttttgaaagt
     3181 aagattaacc caactttcca aacatgaata acataacgtt aaagtttagt taaaaacagg
     3241 ctgatattgg ttagtttgat agtcaaattc agtcgattaa aattgtaaca tattggattc
     3301 caatagatgt tgtttcagag aacacatttg tagtatccct ggattttggt gggaattaca
     3361 ggactttgcg gaataaattt cgatttatca gtatgcgcag accagataca aaaatgccca
     3421 agagttattt tcgtatcaag gaaaagtacc gcgatatagc ggaagtagtt cgcagtcgta
     3481 ttctcttggt ttcgggcttc atcctcgaat tggacgccaa ccaacgaaag caggtagatc
     3541 aattattgac ctattggaaa cagactcaaa aggcatggta tcaagtactt tatgaccaaa
     3601 ataacaagaa tcaggtcaac tgaaaaagtg ctgaccacta ctgccgaaaa taaacttgga
     3661 gaacaagtag cttttagaat attgtactat gccccaaact caagctgcag aaatttacga
     3721 agcgctaaaa tacaaacaac aaccattcaa actacgaaaa atttgtagta cttaaattca
     3781 tccgccagac agtatattta cttgtttagt aatgagttcc gcttaatgaa tggaaagttg
     3841 ggttaaaaat aaaatgtaga gtcaaacagc aattgcagca aactcaatgg gctcttttac
     3901 aagtttatca taaaaatttt tgtttaattt attaattacc actttactga cccattattt
     3961 accgatgtat tgtattataa ttttttacaa aattggtaaa ttcgacaatt aaatcgggat
     4021 tttgttcaac agcgttgccc acgacagcca agtcggcacc tgcgtcgaag cagtcattaa
     4081 gatgttgtgt ggttttcatg cctccgccta caatcatcgg tatggaaagg tgagatgtta
     4141 catggttaat cagttctttt ggaacgggtt gaattgctcc gctgccggct tcgagataaa
     4201 caacatgctg acccgacaat tcgccggcaa ccgctgttgc cagaacaatt tccttttttt
     4261 cgcgaggaat ggaacgtgta tgactcatgt attctacagc actaactttt cccccgtcga
     4321 taagaatgta accggcagga atcacttcga ttcccgattg tttgatagca agcgccgagg
     4381 ttacatgctg tccgattaaa aattcaggat ttctgcccga aattagtgaa aggaaaagca
     4441 atgcatcggc tttcggagaa aattgggaag cattgcccgg aaaaagtaca acaggaattt
     4501 gtacttcttc ttggagaagc tcaataaacg aatctatcgg agaattgacg taactgccac
     4561 ctacaaatac aaaatcggga gccgaaagtt ttaatgcagc aatggttgac gacagcagtt
     4621 ttccctgaca acgatccgga tcgagcaaaa cggccagcat ttttttgttg cattcccgat
     4681 ttatcaatat ttgtttgtac aaatttttca tgattccctt tttgtatgat ttttgtcttt
     4741 tatcctttat cgtgataaat tttcataatt tgtttggctc aacattttta ttaataagat
     4801 aaaccattgc atattcttcc gataattcgt aattgagttc atacaactta ttacgaggaa
     4861 tgtgcaaggc tttgattttt ccggtttttc ccatttcgaa tggaagaata cacaactgac
     4921 gagaaaaatc gactgcttct tttccaataa ctttaaacag cgattccttt gccgaccaca
     4981 acaaggtcag ttttttaagt ctgttttcat ctttttcaag gattttctcc aattcctctt
     5041 tgctcaaaaa tcgcttggaa attgccgata ttttgggttg aaagcgttca atatcaattc
     5101 caactttgca agtaggatgt gttatcacgg cgatccattg accactgtgg gaaataccga
     5161 tttcggcaaa ataatccgaa agaaaaggtt tgccatcgtc gttgtaacag atttttattt
     5221 ctctgttcag caaagttttc aatgctaaac gtaccgaaag atattcgatt tttcgtttct
     5281 cggagtgtaa ggtgtttaac tcttcagttt cgtttttatc aaagaaagtc accagctcgt
     5341 cgaacgtttc agtactttgc caaaccagaa tttttgcgcc ggatgtttca atttttttgt
     5401 gaatcattgt ttccattcaa aactttccat caacctgata atgtcttgcc gaatgtaatc
     5461 cgacatggga gctatggaat ccttgttggg tacattgtcg aaataaagtg agcccctgaa
     5521 aaagtgatga atgctgtcgg ttacaaaaaa ctgaaccgaa gaagcggtat ttcctttcaa
     5581 gtcgtaaaga attccgtaaa cttttttttc gggatgctga taaagtttct cattaatggc
     5641 atcggcacga atgctgtgtt tataagccat ttgtcgtgta tcctccaaca attccttcaa
     5701 atttccatga atgggtttgt aactgcagta aatggccgca ttcagaaaag gatattttat
     5761 atcgatccag catttttctc cttcagcttc tcttttttcc actttggcgt agctcgaaat
     5821 ttcaaatcgg taaggtagtt gaagcgtatc gagataacga taattgtgag gaggtaaatc
     5881 taccctgaaa tatccatagg gacggggaac ataatttttt ccgcaggaga acaaagaagc
     5941 cgtgataacg ataaggagcg tcgttttata aaatattgtt tgcaagtatt tgattttctt
     6001 tgtttccatg aagttgccga ttgttttttc atcaaaaaaa cagcttgata ttcaaatcaa
     6061 aaagttgttt taattttctt ctttgttgcg atcgaaatta tctttcatat gaagtttaat
     6121 ttttcggatt ctgcggttgt ctaccgacaa gatttcaaaa acgtaacgtc catattcgat
     6181 catttcattt tttgcaggca tttctccttt cagttcaagt accaagccgg ccaaagtttc
     6241 cacttcttca gtaacttttg aaaattcctc ttcgttgata cccgtaactt taaaaaagtc
     6301 gttcagcaaa attttcgctt caaaaatata ggtgtgattg tccactttgg tataaagttt
     6361 ttcttcgtcg tcgtattcat cactgatgtc gccaacaatt tcttccaaaa tatcttccag
     6421 cgtaaccagt ccggaaattc caccatattc gtcgaccacc aaagcaagat gaactttgtt
     6481 tttctgaaat tcgctgagca gatcgtcaat tttttttgtt tcgggtacgt agtaagcttg
     6541 cctaattaaa ctttgccatc tgaaattggc aggtttgtca agatggggca gcaaatcctt
     6601 actgtaaaga atgcccttta tattgtctct gttttcagaa taaacaggaa tacgagaata
     6661 acccgtttgg atgataattt tcaataaatc cttgaaattt gtctttgtat ctacagctat
     6721 catatccact cgcgaagtca taatttccgc caccctgata tttccaaatt ttataatacc
     6781 ttcgagcatt tctttgtcat catcttgctc atcagtggta agttccagcg catgagagag
     6841 ttcatcgatc gatatttcgg cttttttata tttttctatc tgacgggtaa gaaaagcagt
     6901 agaagaaatc agcaatttgg aaacccgact aaacactttt tgcataacca aaataaatgg
     6961 agtaaacaat aatgccattt tttcgggata ttgcgatgcg taaaccttag gtatgatttc
     7021 accaaaaaga agcaatacaa aagttatgat caccgtttcg aaaataaatc ccagcaaagg
     7081 aaaagtagaa aaatcgaaca cggagcctgt aaaatacgtc agcaacatga taatggccac
     7141 attaacaaaa ttattcccaa ccaaaatggt tgccaacagc tgttgaggat tgccaattaa
     7201 gtgcatcaga ttttttgcgt tttttctgtt gctctcttca acttcatttt tcatctgtgg
     7261 agtaagagaa aaaaaagcta cctccgaacc tgaaataatt gccgaacaca ttagcaataa
     7321 tatactcaac aaaagtgcaa ttgcggccga aacagaaaaa gaatgaaatg ctatcattgc
     7381 attaatatgt gataaaagaa tagttattat cttaattcta aaacagttag tggtagaaat
     7441 ttttttactc tcataccaaa gaacttgtct atttcaaata taatttcttg caactaagcc
     7501 ttaacaaaca aaaaaattct taaaacaaat ttgcttatag aagcgtttta tgtaaaggga
     7561 aacgctttaa agctgtttta aaaaagttta ttttaaatgc aaatataagg ttaatttctg
     7621 tttttagaaa aaaaacaaaa actggatgat acatatcata aaaattatta actttacacg
     7681 aaaatgaaag gataggacaa aggaaagttt cctattaaaa actaaaatct tagaaatcat
     7741 ggaaaataaa tttgtaacgc tcgctattcg cacttacaaa agggctcaaa tgatcaaaac
     7801 aacgctcgaa gaaaacggga tagaaacccg gatagaaagt gtgaatctgg aacatcccga
     7861 aatgtcggta ggtgttcgta tcagaattaa agaaaatgac cttccccgtg ctctgaaaat
     7921 tgtagaggaa atggaaattg cgtgggaaga aaaaggggaa actcttaaaa gagcagaaaa
     7981 aatgatactg ctgcctatag atttttctga tcagttgcct aaaattatcg attttggttt
     8041 ttattttgca gaatctttaa atgccaaggt cgaactgctt tatgtttact atagccctcc
     8101 ttttacaatt tcttcggtaa acaacgaagt caacacttat actctgagcg atagcgaatt
     8161 actccgtcaa atcatttcaa atgctaatcg tgaagtaact tccatgacca ataccattaa
     8221 cgaacgaatt gctaaaggag aattgcctaa tgtccctttc cactttgaac tcagggaagg
     8281 cgtaccggaa gatcaaattg ttgacttttg taaaaaatat caacctgctt tggtgatcat
     8341 ggggacacga gggaagaaga tcagcagtga gcttatcgga agtgtaacag cagaagttat
     8401 agaatcttgc gtatctccgg tattggctct ccctgttgac atgaaaatta atacaccttc
     8461 cgaaatcaaa cggatagctt tcttgacaaa tttcgaacaa aaagacctta tagccattga
     8521 caataccatt tcacttttta attggaattc cctggaaatg ttctttattc acgctgcaca
     8581 aaaaaaggaa gtatgggacg aagtgatgct cgcaggaata aaagaatact tctccaaaca
     8641 ttaccctaaa attattactc attatgaatt aatagattct accgaaacca tgaaacaaat
     8701 tgacgattat ttggtcaaac acgatattaa tttgctggca ttcaattctc cacgccgcaa
     8761 ccttatccga cggctattta atcccggttt agctttcaaa atggttttac attcggatac
     8821 acctttgttt gtaacgcaca tttcataaac attttcaatt tgcaatgttt gttgttagat
     8881 tttaacccag attattcatt agccaattta aagggaaagt caagttcaga acaactgttc
     8941 cacactctgg aaaaaccaga ctcgcttttt aattttaaag cgattttgag aaccattttg
     9001 ttgttgattt ttcaaaatat gcacttacgg caaaagttca aatcagcagt atggatatag
     9061 tttttttgcg cctttgcctg ccaaataagc attctaaaca gcgacataag atgatgggct
     9121 atcctcacaa aaattaaagc tgcttcagtt acataaaaaa gacttttagg ttaaaactgt
     9181 caaagacaaa gtcatatttt aatttcttta ttttgtcttc agcacttttc tcttcttcgg
     9241 tatattctcc atatctctct ggtgctgaaa gtttcatggt ggttacaaag cagaataaga
     9301 ataacaataa tccttgtaaa cttcttcctc ctcaaaattg gttggtaacc tgtgtgaact
     9361 tggggaagaa gcgtttataa atgtcttgag ccggtgtatt ttttcaacca aaatcttccc
     9421 ctgtgctgca tcgtttcggg taaattctat atgtaaaaat tgattgtctc cacaccatac
     9481 accgattatg aatccttcaa taagagaagt tgtttttata gctccgattt gaagctgaaa
     9541 ccgataaatt gagtttactt gcttcaaagt tcataaacgt cattttatat tgccgggcat
     9601 ttgagttttt tacaaaatta aattaaaact ttattttcct actgtgaatt ctgtgcttaa
     9661 aaaaatggac acctgcaaag tctttgacag aacggttacg tgatcgtgtt gcgataaaaa
     9721 atctaaaaaa gagtttttta ccgagaacat ttactcgatt tcctgaaatt tttcgagatt
     9781 taaaattttc tcttcccact ccaacatagc aacttttctt tcccttcccc aacgaaattc
     9841 tccaatactt ccgtttttaa gaatcactct atgacaggga accagaaatg ctatcggatt
     9901 gcacgctata gcatgagcta ctgagcgaac tgctttaggc atgccaatac ttatggcaat
     9961 ttcttcataa gaaactctac ctccaaaagg aatcttcagc aaggcttccc agacggtttt
    10021 ctgaaaatct gatccataaa aatttaatgc tggacaaata tttctttgga aaatgagctg
    10081 agccattatt cctatttcct tatcatcatg ataaaatttt ttatctggaa atttcccaaa
    10141 caattcattt attgcaaatt cttcatcaaa aggaaaagta agcgaaaaaa ttccccgatc
    10201 atctttagcc acgcaacatt tcccaaaagg ggtcgaagca aaaccaaaac aagcactatg
    10261 gaaagctgtt gtatttatca tcaagcaaat gtgaaaagtt cattactatc cgaaacttta
    10321 gaaattaaaa atgcctgaga atttcatatt tttgtagtga aacaaaacat acaggaataa
    10381 tgaaactcca caggcgcagt aaaaataaca aaaaaccggt tatccgacaa gttttagact
    10441 taatcccaag atttatactt cgtaattcta tacaaaaata tcaaagcgac aaaggttgcc
    10501 ataaatacaa gacttacgac caattggtgg cattaagttt cggacagctt ggtaaatgct
    10561 atactttgtc agatataagt tgctaattat caatcagtag tacctttatg gctgatttgg
    10621 gattaaagca gaatccggtc aaatcaacga tgagcgatgg caacaggaat agggattatc
    10681 gggtatttga agaaatttat tatcagctga ttaagcatta tggacgcaca ttgactg
//
LOCUS       DAOSIB010000074        10145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_6440_length_10145_cov_10.943112, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000074 DAOSIB010000000
VERSION     DAOSIB010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10145
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6440_length_10145_cov_10.943112"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLD44_07005"
     CDS             <1..205
                     /locus_tag="PLD44_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOF99116.1"
                     /translation="KLRNAQLGYSFPHTLITKAKLEKVRFYISGQNLFTIKSKDFSGL
                     DPENPNLAYPISKTVTFGLNVAF"
     gene            246..1922
                     /locus_tag="PLD44_07010"
     CDS             246..1922
                     /locus_tag="PLD44_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOF99117.1"
                     /translation="MKRLLFSVGIILSLYSCTDFLEVPTSGALTDDELTGCEQVENAV
                     ISAYAGIGNDEINRPLSLWNYGNVRADDAYKGGSGATDGEFLHFLEISSPSIGNETWY
                     TDGFWYRNYVAISRANFALKLLNNISEKEMPNKKIRIAEMRFLRGHMHFIQKIVFKMV
                     PYIDESMSPEDIANTSNVALTNDELWQKIADDFQFAYDNLPETQSEVGRANKYAAAAY
                     LAKVFLYKAYRQDEMHNVVSIDKGDMQKVIDLTGIVINSPYRLENDFAYNFLPGSYEN
                     GQESLFAVQYSKDDGTTYGRLNMGNALTTPTPGGDFNKPSQNLVNAFKTNNGLPMFDT
                     YSDVEYNEATDKTDPRLFHTVAIPGKPYKYTNVNFRTDQSRNPNMYGYYSSLKENVDP
                     ASEYYVKTGPWYANSKNTIVIRYADVLLMRAEALIETGDYQDALPLINQIRNRAKVST
                     SLIGFASSYVNIQPYEDGVNCTWTQDYARKALRWERRLEFAMEGSRFFDLVRWGVAAE
                     TINDYFQREKIVRTYIKDDTHFTAGKDEYFPIPQPQINFSGGLYKQNPGY"
     gene            2172..3929
                     /locus_tag="PLD44_07015"
     CDS             2172..3929
                     /locus_tag="PLD44_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HOF99118.1"
                     /translation="MKKFLIPLFAVLVILLAVGFKASKDKQQKKIKITTAYLLLPVEE
                     NADEVKTTIKYPDKKLPDIFWIRFAQRKVDYWVKLEVGKYKGKSLELIFDGPSASKFG
                     IKRIRPADQFKINYAEPYRPDFHFSPEYGWMNDPNGLTYFEGEYHLFYQYNPFGTRWG
                     NMSWGHAVSTDLTSWTYLPVALWPDTLGAIFSGSVVIDKNNTAGFGKNAMIAIYTSAG
                     KVQSQSLAYSTDKGRTFNKFEKNPIIPNPGITDFRDPKVSWYQPENKWIMALATKQTV
                     TFYSSLDLKNWNRESEFGEGIGSHAGVWECPDLFPLNYDGKTKWVLLVSINPGGPNGG
                     SATQYFVGDFDGKKFQADPLPYPLWIDYGRDNYAGVTFSNIPEIDGRRIFLGWMNNWD
                     YANQTPTKIFRSSNTVPRELQLVNNGNHCVLSSFPVKEVDLLRGETVDFPDQIVNNEK
                     SLKEILKQNDGSFELNMVLKPEKGNVFGFSLQNSKGETLDFIFDLSTQFFKIQRNKSG
                     LIDFSDRFPLGMNAPLASKRSYEIRLLIDKCSAELFINHGEIVMTSLFYPREAMNSLK
                     FFSQEGTLKVEDIKVSNLK"
     gene            3942..5303
                     /locus_tag="PLD44_07020"
     CDS             3942..5303
                     /locus_tag="PLD44_07020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HOF99119.1"
                     /translation="MLLNKYKLMKNRYLYWTTFVAINGGLLFGLNMAGISGAVDMIKG
                     EFSLTDSGLGTVAGLLTLGCLLGALFTGNFAEKYGRKKVMISTAIIYIVSALGCSFAE
                     SSGMLIVFRVLSGLAVGASSVVCPMYISEIAPAYNRGRLVSFNQFAITIGILLAYVFD
                     YILAGLGENSWRYMLLIPAIFGVLYLIFLLLKFPESPRWLLSKNKKEEAVKIMKAIGG
                     DELIATELPEMEKTINADIRKEKVKFIELFQGKMGKIVLIGTLLAAFQQITGINAVIM
                     FAPDIFQAAGSAKMDSLMQSMIVGLVNFLMTVVALWLVDKSGRKSLLFWGAIGMILSL
                     AYLTFEFSRPLQNSTGVLIALLVYISFFAASFAPVMWVVISEIYPARIKGLAMSFSTA
                     VSWLCTFLTVYFAPVIQGKLGFEYLFGIFCTFSLLAAVFVKLWIPETKGKSLEIIEKE
                     LGL"
     gene            5330..6220
                     /locus_tag="PLD44_07025"
     CDS             5330..6220
                     /locus_tag="PLD44_07025"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HOF99120.1"
                     /translation="MKDIIVGIGEILWDVLPTGKVLGGAPANFAYHISQLGFDGIAVS
                     AIGNDELGEEIIENLHRKNMNFIIEKVSFPTGTVQVTLNDAGIPCYEICENVAWDNIP
                     FTEEMKKLAQETKAVCFGSLAQRHPVSRQTIQQFLDIVPQEALKIFDVNLRQHFYSKE
                     LIDYSLKKCNILKINDEEILLLSQLFDWNEKSDIDICKRLLRDYSLDMIVLTKGTNGS
                     CVVTPHEISSKPTPIVEVADTVGAGDAFTAGFVAGILKGKSVPDAHELAVEISAFVCT
                     QQGAMPTLPQELINKISSPV"
     gene            6296..9052
                     /locus_tag="PLD44_07030"
     CDS             6296..9052
                     /locus_tag="PLD44_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOF99121.1"
                     /translation="MKKAFLFNLIVLLFFLPSKALPIKKEFLIGVSQCSDDAWRRTMN
                     EEILREASFYPNLKVKIKTAFDSNQKQIRDIESFIEDKVDILIVAPNEAIPLTPVIEN
                     AMKCSIPVILVDRKINSDNYTAFVGADNYQIGKEVGIYVANLLKRKGNIVEITGLKGS
                     TPAVERHKGFRSVIDNYPQIKIVYENDGGWLRDEAREKMKNAIRLNIPIDLVFAHNDE
                     MATGVYEAYQVYNEKEKPKIIGIDALPGTNGGIQKVLDGILDATFIYPTGGEKAVQIA
                     VRILRHEPYDKENILYTAVVDKTNARVIKLQTDQIIQHQNRIAQLNQVLNKNLSQYST
                     QRIFLVLSLTVLFVILLLLALLFKSYKHKNKVNRELEIRNAAINRQKEELAEQRDQLL
                     MLSKNLEEATQAKLVFFTNISHEFKTPLTLILGPLDRLQEKENLSEEGKQLILLMRKN
                     VHLLLKLIDQIIDFRKTETGKMQMTFTFGDLKLFISDICQSFRFLAQKKHIHFNFFSD
                     GDDFVLWFDSDKMEKICYNLISNAFKFTPENGKIEVKLSKYALNDEFFAKIIVSDSGI
                     GIPNSQLDKIFNRFFKIGNNTQNSGIGLHLTKMLVELHNGIIEVQSVEGKGSTFTVTI
                     PFKQKDIAMSEQYPDINFERNYQEKINYMDVSDEINDEYNAEKPLILLVDDNADVRNF
                     IKTLLQNEYTLIEAKDGQSGLLKAMKYVPDLIVSDVMMDKMNGFELCKAVKENISTSH
                     IPVILLTAYAMDEQRSLGFESGADAYIPKPFNENLLKIRIRKTIENRKKLKEYFQNHL
                     AFGDKKELLNEVDNNFLERFRRIIEENLMDTELNIDDIGKSMGLSRVQLYRKVKSLTN
                     YSPNEFIRIIRLKTAEQMMITGEKSISEIAYDTGFTSPSYFTKCFKEYFNENPTDFLK
                     RIKN"
     gene            complement(9416..9754)
                     /locus_tag="PLD44_07035"
     CDS             complement(9416..9754)
                     /locus_tag="PLD44_07035"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HOF99122.1"
                     /translation="MIYEKRGTIPNRTDISQRPPEVEQRERFGDLGIDLIIGKNHKGA
                     ILTINDRATGLGKLRKLESKDAEQLAMATIDCLMEWEPLLKTITPDNGKEFAAHQMDS
                     PTVKYRFLLC"
     gene            9903..10118
                     /locus_tag="PLD44_07040"
     CDS             9903..10118
                     /locus_tag="PLD44_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99123.1"
                     /translation="MIQKFRGKNKVVKTIGCATTQHEIERLEQLVREEMNRYKIIVET
                     FIFYIFRYLITGLCPKLHLLFCLETKK"
BASE COUNT         3329 a         1568 c         1960 g         3288 t
ORIGIN      
        1 gaaattgcgt aatgcacaac tgggttattc ttttccacat actttaatta ctaaagcaaa
       61 actcgaaaaa gtacgatttt atataagcgg acaaaatctg tttaccatta aaagtaaaga
      121 cttctcagga cttgatcctg aaaatccgaa cttggcatat ccaatttcaa aaactgttac
      181 attcggatta aacgtggcat tttaaatatt ttataaaaat aaaaattgaa aatatgaaaa
      241 ctaatataaa aagattatta ttctcagtag gaattattct ttctttatat tcttgtacgg
      301 attttttaga agtacctact tctggagcat taacagatga tgaattaact ggatgcgaac
      361 aagtggaaaa tgcagttata tctgcatatg ctggtattgg gaatgatgaa ataaataggc
      421 ctttaagttt atggaactat ggaaacgttc gcgctgatga tgcttataaa ggaggtagtg
      481 gggccaccga tggagaattt ctacattttc ttgaaatatc ttctccatcc ataggaaatg
      541 aaacttggta caccgatggg ttttggtata gaaattacgt ggcaatttca agagctaatt
      601 tcgctttgaa attattaaat aatatcagtg aaaaggaaat gcctaacaaa aaaatacgaa
      661 tagctgaaat gcgattttta agagggcaca tgcacttcat tcaaaaaatt gtattcaaaa
      721 tggttccata tattgatgaa tctatgtctc ctgaggatat cgcaaatacc tcaaatgttg
      781 ctttgacaaa tgatgaattg tggcaaaaaa tagctgatga ttttcagttt gcttatgata
      841 atctgcctga aacacaatct gaagtaggac gtgcaaacaa atatgccgcc gcagcgtatt
      901 tagcaaaagt gtttttatac aaagcttatc gtcaagatga aatgcacaat gtcgtttcaa
      961 ttgacaaagg tgatatgcag aaagttattg atttaacggg catcgtaata aattcaccat
     1021 atagattaga aaacgatttt gcctataatt ttcttccagg aagttatgaa aatggacagg
     1081 aaagtctctt tgctgtacaa tactctaaag atgacggaac cacttacgga cgtttgaata
     1141 tgggtaatgc tcttacaacc ccaactcccg ggggagattt taacaaacct agccaaaatt
     1201 tggtaaatgc atttaaaaca aacaatggat taccgatgtt tgatacttat tctgatgttg
     1261 agtataatga agcaaccgac aaaacagacc cgcgattatt tcacacggtg gcaatacccg
     1321 gtaaaccata taaatatacc aatgtaaact tcaggacaga tcaatcccgc aatccgaata
     1381 tgtatggata ttattcttca ttgaaggaaa atgtagatcc agcgagtgaa tattacgtaa
     1441 aaacaggtcc gtggtatgca aattcaaaaa atacaattgt tatccggtat gctgatgtac
     1501 tattaatgcg tgctgaagca cttatcgaaa caggtgatta tcaagatgca ctgcctttga
     1561 taaatcaaat ccgaaaccgt gcaaaagtga gcacaagttt aattggtttc gcctcttctt
     1621 atgtaaatat tcaaccctat gaagacggtg taaactgcac ttggacacag gattatgcac
     1681 gtaaagcatt acgctgggaa cgacgtttgg aatttgctat ggaagggagt cgcttctttg
     1741 atttggtacg ttggggtgtt gctgcagaaa ctataaatga ttatttccag agggaaaaga
     1801 tagttagaac ctatattaaa gatgatactc attttactgc tggaaaagat gagtattttc
     1861 caattccaca gcctcaaatt aatttttctg gtggattata taaacaaaat cctggttatt
     1921 agttattttg aatagcaatc atctactcta aaaaagtaga tgattgctat ataatataaa
     1981 atatagcgta ttataaattt ggattcaatg aataaatttt ttgttcagaa gtaattgatt
     2041 tttactgttt ctcccgttgt aaattcattt aatgggagaa acagttattt gttgcaattc
     2101 attgaaaatt aattccaaaa aaagtattct tactaattgt tagaaattta aggtaacccc
     2161 agaaaacttt tatgaaaaaa tttcttattc ctctatttgc tgttttggtt attcttttgg
     2221 cggtaggatt caaggcttcg aaagataagc agcaaaagaa aattaaaatt actactgcct
     2281 atttattgtt acccgttgaa gaaaacgctg acgaagttaa aaccacaata aaatatcctg
     2341 ataaaaaatt gccggatatt ttttggattc gttttgctca gcgtaaggtg gattactggg
     2401 taaaattaga agttgggaaa tataagggca aaagcttgga attaattttt gatggtcctt
     2461 ctgcatccaa gttcggaata aagaggataa ggccagccga tcaattcaag attaattacg
     2521 ctgaacctta tcgtcctgat ttccattttt ctcctgaata tggttggatg aacgatccca
     2581 acggtttgac ttattttgaa ggcgaatacc atctttttta tcagtataat ccctttggaa
     2641 caagatgggg aaatatgagc tggggtcatg ccgtaagcac cgatctaacc tcatggactt
     2701 acttgcctgt ggctttatgg cccgatacat tgggggcaat tttttcggga agcgttgtta
     2761 ttgataaaaa taatactgcc ggttttggga aaaatgccat gattgctatt tataccagtg
     2821 ccggaaaagt tcaatcgcaa tcgcttgctt acagtacaga taaaggaaga acctttaata
     2881 agtttgagaa aaatcccatt attccaaatc ccggtataac tgattttcgc gatccgaaag
     2941 tgagttggta tcagcctgaa aataaatgga taatggcatt agcaacaaaa caaacggtaa
     3001 ctttttattc ttcacttgac cttaaaaact ggaatcggga aagtgaattc ggtgaaggaa
     3061 ttggctcaca tgcaggagtg tgggaatgtc ctgatttatt tccattaaac tatgatggta
     3121 aaacaaagtg ggtcttattg gtaagcatta atcccggagg acctaatggg ggatctgcta
     3181 ctcaatattt cgttggagat tttgatggca aaaaatttca ggctgatcct ttgccttatc
     3241 ctctttggat agattatggg cgagacaact atgcaggcgt cacttttagc aatattcccg
     3301 aaatagatgg tcgtcgaatt ttcctcggtt ggatgaacaa ctgggattat gcgaatcaaa
     3361 caccaacaaa aatttttaga agtagtaaca ctgttccccg agaattgcag cttgtaaata
     3421 atggtaatca ttgcgtgtta agtagttttc cagtaaaaga ggtcgatttg ctcagaggcg
     3481 aaacagttga tttcccagat caaattgtaa ataatgagaa atcgctgaaa gaaatattga
     3541 agcagaatga tggcagtttt gaacttaata tggttttaaa acccgaaaaa ggaaatgttt
     3601 ttggattttc gctacagaat tcaaaaggtg aaacattgga ttttattttt gatttgtcca
     3661 ctcaattttt taaaattcaa aggaataaaa gcgggcttat tgatttcagc gatcgttttc
     3721 cattgggaat gaatgctcca ttagcgtcta aaagaagtta tgaaattcgt cttttaattg
     3781 ataaatgttc tgcagaattg tttatcaatc acggtgaaat cgtgatgacg tcgctttttt
     3841 atccaagaga agcaatgaat tctttaaaat tcttttcgca agaaggcacg ttgaaagttg
     3901 aagatataaa agtcagcaat ttaaaataaa aaatcattca aatattatta aacaaataca
     3961 aacttatgaa aaacagatat ttatattgga caacttttgt tgctatcaat ggaggattat
     4021 tattcggctt aaatatggcc ggaatttctg gagcagtaga tatgattaag ggtgaatttt
     4081 cactcactga cagtggactt ggaacagttg ccggattgtt aacacttgga tgtttgttgg
     4141 gagctttgtt taccggaaat ttcgcagaaa aatatggtcg aaaaaaagtg atgattagta
     4201 cagctattat ttatattgtt tcagccttgg ggtgttcctt tgccgaatct tcaggcatgt
     4261 tgattgtttt tcgtgttttg tcaggattgg ctgtaggtgc aagttctgta gtttgtccca
     4321 tgtatatatc ggaaattgct ccggcctaca atagaggtcg actggtttct ttcaatcagt
     4381 ttgctataac cataggtatc ttattggctt atgtatttga ctatatttta gcaggtttag
     4441 gtgaaaacag ctggcgctat atgctcttga ttcctgcaat ttttggtgtg ttgtatctga
     4501 tttttcttct tttaaagttt cctgaaagtc ctcgttggtt attgtcaaaa aacaagaaag
     4561 aagaagctgt gaaaataatg aaagcaattg gaggagatga attgattgct actgaacttc
     4621 ccgaaatgga aaaaacgatt aatgctgaca ttcgtaaaga aaaagtcaag tttattgaat
     4681 tgtttcaggg aaaaatggga aaaattgttt taattggaac tttattggct gcttttcaac
     4741 aaattacagg aataaatgca gttattatgt ttgcacctga tattttccag gcggcaggtt
     4801 cagccaaaat ggattcattg atgcaatcga tgattgtggg gttggtcaat tttttaatga
     4861 cagttgttgc tctttggtta gtagataaaa gtggtcggaa atctctttta ttctggggcg
     4921 caatcggtat gatcctttct cttgcatatt tgacatttga attttcacgt cctttacaaa
     4981 atagcacggg tgtattgata gctttattgg tttatatttc tttttttgct gcttcttttg
     5041 cgccagttat gtgggttgtt atttctgaaa tttatccggc aagaattaaa ggtttagcca
     5101 tgtcattttc tactgcagta agttggttat gtacttttct aaccgtatat tttgccccag
     5161 ttattcaagg aaaacttgga tttgaatatc tttttggaat attttgtacg tttagcttat
     5221 tggcagcagt ttttgtgaag ttatggatac cggaaaccaa aggaaaatca ctggaaatta
     5281 ttgaaaagga attgggttta taaaattaat ttggaaatta tcaaaaaaca tgaaagatat
     5341 tattgttgga attggagaaa tactttggga cgttttacca acaggaaaag ttttaggagg
     5401 agcgcctgca aattttgctt atcacatttc tcaattggga ttcgatggca ttgccgtgag
     5461 tgctattgga aatgatgaat tgggtgaaga aatcattgag aatcttcata gaaaaaatat
     5521 gaatttcatt atcgaaaaag tttcttttcc aaccggtacc gtgcaagtta cactgaacga
     5581 tgccggcatt ccatgttatg aaatttgtga aaatgtggct tgggataata ttccttttac
     5641 ggaggaaatg aaaaaattgg cacaggaaac aaaagctgta tgctttggtt ctctggctca
     5701 acgtcatcct gtttcaagac aaaccattca gcagttttta gatatcgtac cccaagaggc
     5761 attgaaaatt tttgatgtta atttgcgcca acatttttat tctaaagagt tgatagacta
     5821 ttcattgaaa aaatgcaaca tactgaaaat caatgatgaa gaaattttgc ttttgtctca
     5881 actattcgat tggaacgaaa agtcggatat agatatttgt aaaaggcttt tgagagatta
     5941 ttcactcgat atgatcgtgc tcacaaaagg aacaaatggc agttgtgttg ttacacccca
     6001 cgagatttca tccaaaccaa ctccaatagt tgaggttgcc gatacggtag gtgccggtga
     6061 tgctttcact gccggttttg tggcaggaat tctcaaggga aagagcgttc ccgatgctca
     6121 tgaattggct gtggaaatat cggcgtttgt ttgtacccaa caaggtgcca tgcccactct
     6181 tcctcaagaa ttgattaaca aaatttcttc cccagtttga tttttatttg gtaaaaaatc
     6241 atatttttgt gttgtaaatc attttgatac ctataatgaa agttttggct gtttcatgaa
     6301 aaaagctttt ctttttaatt taatcgtttt attattcttt ttaccatcaa aggcattacc
     6361 gataaaaaaa gaatttttga ttggtgtgtc tcagtgcagc gatgatgcat ggcgacgaac
     6421 gatgaatgaa gaaatattga gggaagcttc tttttatccc aatctgaaag tcaaaattaa
     6481 aacggctttt gacagcaatc aaaaacagat acgcgatatc gaaagtttta ttgaagataa
     6541 agttgatatt ttaattgttg ctcctaatga ggcaattcct ttaacaccag taattgaaaa
     6601 tgccatgaaa tgtagtattc cggtaatatt ggttgaccgg aaaatcaatt ccgacaatta
     6661 taccgcattt gttggtgccg ataattatca aattgggaaa gaagtcggaa tatatgttgc
     6721 caatttactg aaaagaaaag ggaatattgt tgaaataaca gggctgaaag gctctacccc
     6781 agctgttgag cgccataaag gtttcagaag cgtgattgat aactatcctc aaataaaaat
     6841 tgtatatgaa aatgatggag gttggttaag agatgaagca agagaaaaaa tgaaaaatgc
     6901 tatccgttta aacattccca tagatttggt ttttgctcac aatgatgaaa tggcaacagg
     6961 agtttatgaa gcttatcagg tttacaacga aaaagaaaaa ccaaaaatta tcggaattga
     7021 tgcacttccg ggtaccaacg gtggtattca gaaagttttg gatggcatac tggacgcaac
     7081 ttttatttat cctaccggag gtgagaaggc agtccagatt gctgtaagaa ttctccgtca
     7141 tgaaccttat gacaaggaaa acattctcta cacggctgtg gttgataaaa caaatgcaag
     7201 agtaataaaa cttcaaacag atcaaattat tcagcatcag aatcgtattg cgcaacttaa
     7261 tcaggtgcta aataaaaatt tgtcacaata cagtacgcaa cgaatttttc ttgttctgtc
     7321 gctcacggtg ctttttgtta ttttattgtt gctggcatta cttttcaagt catataagca
     7381 caaaaataaa gtgaatagag aattggaaat cagaaatgct gctataaaca gacagaaaga
     7441 ggagctggcc gaacaacgag accaattact gatgctttct aaaaatctgg aagaggccac
     7501 acaggcaaaa ctggttttct ttaccaatat ctcacacgaa ttcaaaacgc ctttaacgct
     7561 tattctcggc cctttggatc gattacagga aaaagaaaat ttatctgagg aaggtaaaca
     7621 attgattctt ttaatgcgca aaaatgtgca cttgcttctt aaattaattg atcaaattat
     7681 tgatttccga aaaaccgaaa ccggaaaaat gcaaatgact tttacttttg gcgatctaaa
     7741 actttttatt tcagatatat gtcagtcttt ccgtttttta gcccaaaaga agcacattca
     7801 ttttaatttc ttttctgacg gtgatgattt tgttttgtgg ttcgatagcg ataaaatgga
     7861 gaaaatttgt tataacttga tttctaatgc tttcaaattt actccggaaa atggtaaaat
     7921 tgaagtaaaa ttatcaaaat atgctctcaa cgatgaattt tttgcaaaaa taattgtgtc
     7981 cgattcgggc ataggaatac caaactcgca gttggacaaa atttttaata gatttttcaa
     8041 aatcgggaac aacactcaaa attccggaat tgggttacat ttgacaaaaa tgttggtcga
     8101 attgcacaat ggaatcatag aagtacaaag tgtcgaaggt aaaggttcta cttttactgt
     8161 aaccattcct ttcaaacaga aagatatagc tatgtccgaa cagtatccgg atattaactt
     8221 tgaacgaaat tatcaggaga aaattaatta catggatgtg tctgatgaaa ttaatgatga
     8281 atacaatgct gaaaaacctt tgattttatt ggtagatgat aatgctgacg ttcgtaactt
     8341 catcaaaaca ttgttgcaaa atgaatatac tctcattgaa gccaaagatg gacaaagcgg
     8401 attgctcaaa gcaatgaaat acgtacccga tttgattgtt agcgatgtga tgatggataa
     8461 aatgaatgga tttgaattgt gcaaggcagt aaaagaaaat atttctacca gccatattcc
     8521 cgttatttta ttgacagctt atgctatgga cgaacaacgc tctttgggat ttgaaagtgg
     8581 cgccgatgct tacataccga agccttttaa tgaaaatctt ttgaaaatca gaattagaaa
     8641 aaccattgaa aacagaaaaa aattaaagga atattttcag aaccacttgg cttttgggga
     8701 taaaaaagaa ttgttgaatg aagttgataa taactttttg gagagattca gaagaattat
     8761 tgaggagaat ttgatggata ccgaattaaa tattgatgat attggcaaat ctatgggttt
     8821 atcacgcgtc cagctttacc gaaaagttaa atcattgaca aactattctc ccaatgaatt
     8881 tattagaatc ataaggttaa agactgccga gcaaatgatg attacaggtg aaaaaagtat
     8941 ttcggaaatt gcttacgata ccggttttac ttcaccttcc tatttcacca aatgttttaa
     9001 ggagtatttc aatgaaaatc ctaccgattt ccttaaacgt attaaaaatt gattttaaat
     9061 tatttcaatt tttgtttgca cacctgttgt atcattgccc aaattgtgta cttttgttat
     9121 actataaaaa tgtttcatct tgtaactttt taaaatggaa gagggcgcag taaagcattt
     9181 atttcaacag ggaaggggga agaagagttt ttttctttgt cttcaaaaaa attatttccc
     9241 tttcctttca tttcccctga aaaagtcgca ttaatcactt gaatttacgt ttgcgtttcg
     9301 acaaataata taatcctgag agatacattc aaaatcgact tttttcggta tatattgcct
     9361 aacgaggcga ttataatttt cattcgatcc tcttttttgc cagcttgcat atggtttagc
     9421 aaagaagaaa tcgatattta actgttgggc tatccatctg atgagcggca aactcttttc
     9481 cgttgtctgg agtgattgtt ttaaggagtg gttcccattc cattaaacaa tcaatggtag
     9541 ccattgcaag ttgttcggca tctttgcttt ctaatttacg aagtttccct aatccggtag
     9601 ctctgtcatt gatggttaag atagcgcctt tgtgattttt gcctataatc aaatcaattc
     9661 ccaaatcacc aaaacgttct cgttgttcaa cttcaggcgg tctttgagag atatctgtac
     9721 gattggggat tgttccccgt ttctcgtata tcatacttcg tttttcctgt tttccacatt
     9781 tttttgcaac acccttataa gtatttgaat atcaataata atattttaat tttgggtgtt
     9841 gcaaaattat tattatgttt atccgaaaag taaaaaatcg atctggaagt caatctgtac
     9901 aagtgataca gaagtttcga ggaaagaata aagtagtgaa aaccattggt tgtgccacca
     9961 cgcagcacga aattgaaagg ttggaacaat tggtcagaga agaaatgaat cgttacaaaa
    10021 taattgtaga aacatttata ttctatattt tcagatattt aataacgggg ctttgcccca
    10081 aactccactt acttttttgt cttgaaacaa aaaagtaagc aaaaaaatta acttagttga
    10141 agcag
//
LOCUS       DAOSIB010000075         9570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_6881_length_9570_cov_11.097320, whole genome shotgun sequence.
ACCESSION   DAOSIB010000075 DAOSIB010000000
VERSION     DAOSIB010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9570
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6881_length_9570_cov_11.097320"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..410)
                     /locus_tag="PLD44_07045"
     CDS             complement(27..410)
                     /locus_tag="PLD44_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99124.1"
                     /translation="MKRKLFILGLMIFTAMSLSAQLPKFMELSPEVRAKTHTEWMITT
                     LKLNPNLSEKIYSINLQFAKEVEPVKSDEISKFKKIKIIRNADGERLKQFKTFMTPDQ
                     VETYIQKRKELFDEERKREKESAKN"
     gene            complement(584..1285)
                     /gene="tsaB"
                     /locus_tag="PLD44_07050"
     CDS             complement(584..1285)
                     /gene="tsaB"
                     /locus_tag="PLD44_07050"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOF99125.1"
                     /translation="MNLPLILHIETSTQVCSVALSSGTECVFSKSSEEQGMNHAKLLS
                     GFIAEAMNVLNNDFHSLNALAVSSGPGSYTGLRIGVSTAKGLCFGLDIPLISIGTLDI
                     MAVSALNSVNEKDALLCPMLDARRMEVYAAVFNTDLEMIQPVAANIVDERTFNEILKQ
                     NSVYFFGNGMSKCKPVLQQHARAHFIDEIIPLAENMIPLALKAYQKKQFVDTAYFEPF
                     YLKEFQTTVSKKKMF"
     assembly_gap    1471..1570
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1769..1936
                     /locus_tag="PLD44_07055"
     CDS             1769..1936
                     /locus_tag="PLD44_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99126.1"
                     /translation="MSDNGDLLYKSKDGNLLQINHYDTRQLKLTVRYNFNTTKSKYKG
                     TEAGEKEMKRL"
     gene            complement(1962..2483)
                     /locus_tag="PLD44_07060"
     CDS             complement(1962..2483)
                     /locus_tag="PLD44_07060"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOF99127.1"
                     /translation="MKFVRVLFSGLIVLCFLNGCRKTPPQLPANKNLDTTNNEVVALV
                     ELNEKMALKEDSVLKQFVKKLKGNFVKSPVGIWYFIENQTNGTLLENSDSCFISWQMF
                     SLEGKLLDEKSHEKVIIGKKQIPVGLEEGLRLMHKGESAVFVVPWYLAYGMKGLPPDV
                     PPYTSLIYKVKFE"
     gene            complement(2487..4922)
                     /gene="thrA"
                     /locus_tag="PLD44_07065"
     CDS             complement(2487..4922)
                     /gene="thrA"
                     /locus_tag="PLD44_07065"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HOF99128.1"
                     /translation="MKVMKFGGTSVGSTEAVLNVKKIVESQKESVIVVVSALSGVTDQ
                     LYRISQLAASGNETYLDEYNQMFARHMQMIAETVLPEKQTEVELQIQEIFTDLLNIFR
                     GIFLIKDTSVKITDTVVSYGEMISSVLIANIIRGAVHFDSRRFIKTQNQFDKHIVDFK
                     TTNELIQQIFKDAPSIVVCGGFIATDVHTNQVTNLGRGGSDYTAAILAAALNASILEI
                     WTDVDGFMTADPRIINNAYVIDRLSYSEAMELSNFGAKVIYPPTIFPAFHRNIPIVIK
                     NTFRPEASGTYISQEKSGEKSKAIKGISSINDTSLITIRGLGMVGVIGVNYRIFRTLA
                     KNGISVFFVSQASSENTTSIGVKNADSALAVEVLKKEFANELELGEIDEIFSEDDLAT
                     VAIVGDNMKRTPGIAGKLFGTLGRNGINVVACAQGASETNISFVTDRSSLRKALNVIH
                     DSFFLSEYQVLNIFVVGIGKVGSHLLHQIKQQQPKLMHQKGLKLNVVGIASSKKMLFS
                     REGIDIEHYQDELKLHGVDSTPERIADEIINMNIFNSVFVDCTANAQIADLYQTLLDH
                     NISVVAANKIATSSSYDKYMKLKETARQKGIKFLFETNVGAGLPIISTMNNLTNSGDH
                     ILKIEAVLSGTLNFIFNTLSSDIPFSKAVLMAKEYGYSEPDPRIDLSGKDVVRKLVIL
                     AREAGYQVEQDDVKKNLFIPEKFFKGSLEQFWEQIPELDEAFELRRQELEKQGKRLRF
                     VASMDKGDCQVGLQEIDNTHPFYDLQGSNNIILLTTERYNEFPMMIKGYGAGASVTAA
                     GVFSDIISIANIR"
     gene            5077..5150
                     /locus_tag="PLD44_07070"
     tRNA            5077..5150
                     /locus_tag="PLD44_07070"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5111..5113,aa:Arg,seq:tct)
     gene            5611..6300
                     /locus_tag="PLD44_07075"
     CDS             5611..6300
                     /locus_tag="PLD44_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOF99129.1"
                     /translation="MLIFVTPLHQYLPGYGDMNLRSKIIQESIRADSLTHAMNLQAAY
                     LEIVKDIIKGNIKPDSVVGVDSAFLQQQAQKLIETSKREKEFTKKFEEEEKFNLTSME
                     SSSTNKKTYVFFRPATGNIVSSFNISKKQYGVALQTLPDAAVSSVLNGIVVFADFTFD
                     NQWVIAVQHEDNYLSIYKNNNRLLKNIGEVVRTGEPIAITGSSKGKNGQLYFELWQQG
                     KPINPQNIIIF"
     gene            6346..7518
                     /locus_tag="PLD44_07080"
     CDS             6346..7518
                     /locus_tag="PLD44_07080"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOF99130.1"
                     /translation="MSNTTKQIAVLGSTGSIGKQALQVITDYPDRFSVYALTANNNID
                     LLIKQARKFQPEIAIIANENHFLQLKEALSDLPIKVFAGEESIGQLVEMQAVDIVLIA
                     LVGYAGLKPTMNAIKAGKRIALANKETLVVAGELIEEMVACYHPSIIPVDSEHSAIFQ
                     CLNGEGEKTEVEKIILTASGGPFRTKTSKELESVTIAEALKHPNWEMGAKITIDSASM
                     MNKGFEVMEAKWLFGVAPEQIEVVVHPQSIIHSMVQFIDGSIKAQLGVPDMRLPILYA
                     FTYPERILTTFPRIDFNLLNSLTFEKPDLERFHNLALAYYALDKGGNMPCALNAANEI
                     VVDAFLNSKLRFLQMSEVIETILSKTFYIGHPSYEDYVQTDIITRQITRELIAQVH"
     gene            7644..9134
                     /gene="rseP"
                     /locus_tag="PLD44_07085"
     CDS             7644..9134
                     /gene="rseP"
                     /locus_tag="PLD44_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOF99131.1"
                     /translation="METFLIRALQLILSLSILVVLHELGHFAFARLFKVRVEKFYIFF
                     NPGFSLLRAKKINGKWQFKFFSKNVPSNERPKKDAEGDIVMGEKGKPQTEPIPLSELS
                     DDDWRKYPESTEWGIGWLPLGGYCKISGMIDESMDTSQLKSDPQPWEYRSRPVWQRMP
                     IITGGVLVNFILAMVIYSAILFTWGKEYLPLENAKYGLHFTSVFIEQGFKNGDKILSV
                     DGKSCADLGEVIEKILIDNAKSVTVLRGDSQILTISLPPDFPQKVLASGESNLISYRF
                     PFVVQDVSPESPAKQAKLQPGDSIVGINGKELTIFQDVVSVLTNYRDSLIQLAYYRNG
                     QKEQVTIQLDQTGKLGVAVRPFTEFLETEHVKYGLLASIPAGISLGWETLTSYIKQFK
                     LVFTKEGSKQLGGFGSIGKLFPQTWNWQIFWSMTALLSVILAFMNFLPIPALDGGYVL
                     FLLYEIFTGKKPSDKFLEQAQTIGMLLLLALLIYANGNDIYKAIFK"
     gene            9263..9418
                     /locus_tag="PLD44_07090"
     CDS             9263..9418
                     /locus_tag="PLD44_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99132.1"
                     /translation="MARVLRVTANLKEAISKADVLMNKNLIEGSNERVTEQWIGNARN
                     STVTQEE"
BASE COUNT         3081 a         1747 c         1752 g         2890 t
ORIGIN      
        1 ataaaaaatc aaattctgtc aattattcaa ttctttgcac tctccttttc cctctttctt
       61 tcttcatcaa ataattcttt tcttttctga atataggttt caacctgatc gggcgtcata
      121 aaggttttga attgttttaa cctttctcca tcagcatttc tgataatttt aattttctta
      181 aacttgctta tttcatccga tttgacaggt tcaacctctt tggcaaattg aagattaatg
      241 gaataaattt tttctgacaa attgggatta agttttaaag ttgtgatcat ccattcagta
      301 tgagttttag ctctaacttc aggtgataat tccataaatt taggtaattg agctgaaagc
      361 gacatagctg taaaaatcat caaccctaaa ataaacagtt ttcgtttcat gtttttaaaa
      421 attttaagtg aatccactta gttttttatc aaatttcatt ccattttaaa ttcaaattgt
      481 aaaggcaatt tttaggatgt tgcaatacgc aaaatagaag cgtggagatt tttgccgatg
      541 agctgaattt tatcatatta atcgacaaac ttggcttata atttcagaac atttttttct
      601 ttgatacagt ggtctgaaat tctttcaaat aaaacggctc aaagtaggct gtatcaacaa
      661 attgtttttt ttgataggct tttaatgcca atggaatcat attttcggcc aaaggaataa
      721 tttcatcaat gaaatgtgca cgtgcatgtt gctgcagcac aggtttacat ttactcatgc
      781 cgtttccgaa aaaatatacg gaattttgtt tgagtatttc attaaaggta cgctcatcga
      841 caatgttggc agcgacaggt tgaatcattt ccaaatcggt attgaaaaca gctgcataaa
      901 cttccatccg gcgggcatca agcataggac ataaaagtgc atccttttca tttaccgaat
      961 tcaaagccga aacagccatt atatcgagcg ttccaatcga aattaacggg atatccaacc
     1021 caaaacacaa acctttggcc gtagaaacac cgattcgcaa acctgtgtaa gaacccggcc
     1081 ctgaacttac tgcaagtgca ttcaatgaat ggaaatcatt attcagtaca ttcatcgctt
     1141 cggcaataaa tccacttaat aattttgcat gattcatgcc ttgttcttcg gaagattttg
     1201 aaaaaacaca ttctgtgcca ctactcaaag ccacagaaca aacctgtgtt gaagtttcaa
     1261 tatgaagaat cagcggtaaa ttcataattt tatattgatg atgaaaaaac agcatcataa
     1321 actgccgttt tctgttggca aaagtagtaa ttttttgctg tagcaagaaa gattttcgtt
     1381 atcaagctat ataaaatatc actactgccc gataaaaaat tggaataggt tagaataaaa
     1441 agggaagtta cctttaaatt taataacctc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn aaaattggaa taggttagaa taaaaaggga agttaccttt aaatttaata
     1621 acctctcctg tgacaacatt agttcgttgc ttttcacggc tcagttaaac ttaaacctgc
     1681 aattttgaag gatggtctgt tttgaaaatt aaaacaaaaa agttatatta taggggttac
     1741 aagtataagt atcgactgaa taaaaatttt gtcagataat ggtgatttgc tctacaaaag
     1801 caaagacggt aatttgcttc aaatcaatca ctacgataca cgtcaattaa agttaactgt
     1861 ccgctacaat ttcaatacga caaaaagcaa atacaaagga actgaggccg gtgagaaaga
     1921 aatgaaacga ttgtagaagt gtacaggaaa atcttttatt attactcaaa ttttactttg
     1981 taaatcaacg aagtataagg agggacatcg ggcggaagtc ctttcatgcc ataagccaaa
     2041 taccagggaa caacaaaaac agcgctttcg cctttatgca tcaaacgcaa tccttcctcc
     2101 agaccaacag gaatttgctt tttcccgata attacttttt catgtgattt ttcgtcaagt
     2161 aactttcctt cgagtgaaaa catttgccaa gaaatgaagc aactgtccga attttccagc
     2221 agagtgccat tggtttgatt ttctatgaaa taccaaattc caacaggcga tttgacaaaa
     2281 tttcctttta atttcttaac aaattgtttc agcacactgt cttccttcaa cgccattttc
     2341 tcgtttaatt cgaccaacgc cacaacttca ttattggttg tgtccaaatt tttattggcg
     2401 ggcaattgag gtggcgtttt tctgcaaccg ttcaaaaagc acaaaacaat taatccggaa
     2461 aataaaacac gaacgaattt cattcttcaa cgaatattgg ctatgctgat aatatccgaa
     2521 aaaacacctg ctgccgtaac acttgctcct gccccataac cttttatcat catgggaaat
     2581 tcattataac gttccgtagt cagcagaata atgttgttgc ttccctgcaa gtcgtagaag
     2641 ggatgggtat tgtcgatttc ctgaagtcca acctgacaat cacctttatc catagaagcc
     2701 acaaaacgaa ggcgtttccc ttgtttttcg agttcctgcc ggcgaagttc aaaggcttca
     2761 tccagttcgg gaatttgctc ccaaaattgc tccaatgaac ctttaaaaaa cttttccggt
     2821 ataaacagat tttttttcac atcatcctgt tccacctgat aaccggcttc acgagccagg
     2881 atcaccaatt tcctgaccac atccttcccg ctcagatcga tacgcggatc gggttcggaa
     2941 tatccatatt cctttgccat caatacagct ttactgaaag gaatatcgct actcaaagta
     3001 ttaaaaatga aattcaacgt tccggaaaga acggcttcta tttttaagat atgatcgccg
     3061 ctgttggtga ggttgttcat agtggaaata atgggtaatc ctgcaccgac attggtttca
     3121 aaaagaaatt ttataccttt ctgacgagca gtttccttta atttcatata cttgtcgtaa
     3181 gaagatgaag tggcaatttt attggcagct acaaccgaaa tattatgatc aagcaaagtc
     3241 tggtacaaat cggcaatttg tgcattggca gtacaatcca caaaaacgga attaaaaata
     3301 ttcatattga taatttcgtc ggcaatacgt tcaggagtcg aatcaacgcc atgcaatttc
     3361 aattcgtcct gataatgttc aatatcaatt ccttcgcgcg aaaaaagcat ttttttgctg
     3421 gaagcaattc caaccacatt cagtttcaag cctttctggt gcatcaattt aggctgttgc
     3481 tgttttattt gatgcaaaag atggctgccc accttgccaa ttcccaccac aaaaatattc
     3541 aacacctgat attcggacag gaaaaaggaa tcgtgaatga cgttcaaagc cttgcgcaaa
     3601 gaactgcgat cggtaacaaa agaaatattg gtttcggaag caccttgtgc acaagccacc
     3661 acatttattc catttcttcc cagcgtgcca aaaagttttc cagcaattcc gggtgtacgt
     3721 ttcatattgt cgccaacaat ggcaacggtc gccaagtcgt cttcagaaaa aatttcgtca
     3781 atttctccaa gttccagttc attggcaaat tcttttttca gtacttctac tgccagcgca
     3841 ctatctgcat tttttacccc aatggaagtc gtattttcag aagaagcctg agaaacaaaa
     3901 aatacactga taccgttttt tgccagtgtt ctgaaaattc ggtaatttac accaatgacg
     3961 cctaccattc ccaatccacg aatggtaatg agcgaagtat cgttgatgga agaaattcct
     4021 ttaatggctt ttgatttttc gccggatttt tcctgagaaa tataggttcc gctggcttcg
     4081 gggcgaaagg tattttttat aacaatggga atattcctgt gaaaagccgg gaaaatggta
     4141 ggcggataaa tcacttttgc accaaaattg ctcaattcca ttgcttcgct gtaactaaga
     4201 cggtcgatta cgtaagcatt gttgataata cgtggatcgg cagtcataaa tccatcaaca
     4261 tccgtccaaa tttccagaat ggaagcattc aacgccgcag caagaatagc tgccgtataa
     4321 tccgaacccc cacgtcccaa attggttacc tgattggtat gtacatcggt agcaataaat
     4381 ccgccacaaa ctactataga aggagcatcc ttaaaaatct gctgaatgag ttcgttggta
     4441 gttttaaaat cgacaatgtg tttatcgaac tgattttgag ttttaataaa tcgacgcgaa
     4501 tcgaaatgaa cagcaccccg aattatattg gcaatcagaa cggaggaaat catttctcca
     4561 taactgacaa ctgtatcggt gattttgacg gaagtatcct taatcaaaaa gattcccctg
     4621 aaaatattca acaaatcggt aaaaatttcc tgtatttgca gttcaacttc cgtctgcttt
     4681 tcaggcaaca ctgtttcggc aatcatttgc atgtgcctgg caaacatttg gttatattcg
     4741 tcgagatagg tttcgttgcc ggaagctgcc agttgggaaa tacgataaag ctgatcggta
     4801 acgcctgaaa gcgccgaaac aaccactatc acagattctt tctgtgattc aacaattttt
     4861 tttacattca aaaccgcttc ggtagaacct acggatgttc caccaaattt cattactttc
     4921 attcgtcagg attttatttt ttcttcaaaa aattttaagt tgcaatatta gtgatttttt
     4981 ggcaaataaa ttgcacctta ccacgatttt gagtaaaagt attgcatctt aatcaaaatt
     5041 aaagtacttt tgcatcgcct ttaacaaaag gaatccggtc ccgtagctca gctggataga
     5101 gcaacagcct tctaagctgt gggtcgtggg ttcgaatccc gccgggatca cgaaaaagca
     5161 ggtaaaaatg acaataaatt acctgctttc tttttacaaa aaaaatcggc tatcatggca
     5221 aagcaaactt tttggagaat aaattttcta aaaaaaccaa aaaacatgtt ttgagacaac
     5281 atcatgcttt gttcgtttca caagattcca tgaatgggga ttcagagttt tatcacaaaa
     5341 cttttttacg gcaggtttac atgaatgggc aaaggagttt tatgtaaaat gaaaataatc
     5401 tcttattttt gtatttattt tgtgagcgat tgaacttcaa gaatgaaaag aaaaaaattt
     5461 ttctcttttt ttcaaaaaat ccggtttaaa tatcgtgttt ccatattaaa tgaaaacaca
     5521 ctcgaagaat cgtggcatat tcatttatcg aggttaagtg tttttatata tctttttgtt
     5581 tttactataa taacttttgc gttgttgtct gtgttgatat ttgtaacacc attgcatcaa
     5641 tatttgcccg gttatggaga tatgaacttg cgatcaaaaa ttattcagga atcgatccga
     5701 gccgattctt taactcatgc aatgaatttg caggcggcat atctggaaat tgttaaagat
     5761 attattaagg gaaatattaa acccgactct gttgttggcg ttgattctgc ttttttgcag
     5821 caacaagctc aaaaattaat agagacatcg aaaagagaaa aagaatttac aaaaaaattt
     5881 gaagaagaag aaaagtttaa tttgacaagc atggaaagtt cttcgaccaa taagaaaacc
     5941 tacgtatttt tccgtcctgc aacaggtaat attgtttcct cattcaatat ttccaaaaaa
     6001 caatacgggg tagccttaca aactttacct gatgctgctg tgtcaagtgt tttaaatgga
     6061 atcgtagtat ttgcagattt tacttttgac aaccaatggg taatagcagt tcaacatgaa
     6121 gacaattatc tttcaattta taagaacaac aatcgtttac taaaaaacat aggcgaggta
     6181 gtgaggacag gcgaaccgat agccatcacc ggttcgtcca aaggaaaaaa cggacaattg
     6241 tatttcgagt tgtggcaaca aggtaaaccc attaacccac aaaatatcat aattttttaa
     6301 actaccagat ttttattcct aaaaacataa acaaattgaa ataaagtgtc caacacaacc
     6361 aaacaaatag cagttttagg ctccacagga tctattggga agcaagcctt gcaagtgata
     6421 accgattatc ccgacaggtt tagtgtttat gcactcacag ccaataataa tatagactta
     6481 cttataaaac aggctcgcaa atttcaacca gaaattgcta ttatagcaaa cgagaaccat
     6541 tttctgcagc tgaaagaagc tcttagcgat cttcccataa aagtttttgc tggagaagaa
     6601 tccattgggc agctggtaga aatgcaggca gtggatatcg tattaatagc tttggttggt
     6661 tacgcagggc taaaaccaac catgaatgcc ataaaggcag gcaagaggat tgcattggca
     6721 aataaagaaa cattagttgt agccggcgaa ctaatcgaag aaatggtggc ttgctaccat
     6781 ccttcaatta tacccgtcga ctctgaacat tcagctattt ttcaatgttt aaatggggaa
     6841 ggggaaaaaa cggaagttga aaaaattata ttgactgcct ccggtggacc tttccgtacc
     6901 aaaaccagca aagagttgga atcggtaacc attgcagaag ccttgaaaca tcctaattgg
     6961 gaaatgggag caaaaataac tattgattca gcctccatga tgaataaagg ttttgaagta
     7021 atggaagcaa aatggttgtt tggtgttgca ccggagcaaa tcgaagtggt agtacaccca
     7081 caatccataa tacattcaat ggttcaattc attgatggat cgataaaagc tcaattggga
     7141 gttcccgata tgcgtttgcc catcttgtat gcttttacat accctgaaag aattttaacc
     7201 acttttccac gtatagattt taacctgtta aacagtttga cattcgaaaa acccgatttg
     7261 gagcggtttc acaatcttgc tcttgcctat tatgctttag acaaaggagg gaatatgcct
     7321 tgcgcattaa acgcagcaaa cgaaatagtg gttgatgcat ttttaaacag caaacttaga
     7381 tttttacaaa tgagcgaagt cattgaaact attttatcaa aaacttttta tataggtcat
     7441 ccctcttatg aagattatgt acaaacagat ataataacca gacaaataac cagagagtta
     7501 attgcacaag tacattgaaa cttcagatga gagaaaaaca aaaaatttaa tttataaata
     7561 aacattttaa taatttagac gataaacatg aaaattcgca tgttgaaatt cgttatcgga
     7621 tgaagttgaa tatttagatc aatatggaaa catttttaat tagagcttta caacttatac
     7681 ttagtttgtc aattttagta gtactgcatg aattggggca ttttgccttt gcccgtttgt
     7741 ttaaagttcg tgtggaaaag ttttatattt tctttaatcc gggtttctct ctattacgag
     7801 caaaaaaaat taatggcaaa tggcaattta aatttttttc gaaaaatgtc ccttcgaatg
     7861 agcgtcccaa aaaagatgcc gaaggagata tcgtaatggg agaaaagggg aaacctcaaa
     7921 ctgaacctat accgctttca gagctttcag atgatgattg gagaaagtat cccgaatcaa
     7981 ctgaatgggg aataggctgg ttacctttag ggggttactg caaaatttcg ggtatgattg
     8041 atgaatcaat ggatacatca caactaaaat ccgatccaca gccatgggaa tatcgttcac
     8101 gtccggtttg gcaacgaatg cctattatta ctggcggagt attggttaat tttatccttg
     8161 caatggtaat atattccgcc attcttttta cttggggcaa agaatatctg cctttggaaa
     8221 atgctaaata cggcctacat tttacatcgg tctttattga gcaaggattc aaaaatggag
     8281 ataaaatttt atcggtcgac ggtaaatcat gtgctgattt aggagaggta attgaaaaaa
     8341 tactgattga caatgccaaa agtgttactg ttctaagagg cgacagccaa attttgacta
     8401 tttcattgcc ccccgatttc cctcaaaaag ttttggcttc aggagaaagc aatctgattt
     8461 cgtatcgatt cccattcgtg gtacaagatg tttcaccgga atcacctgca aaacaggcaa
     8521 aacttcaacc gggcgatagc attgtaggta taaacggtaa agaattaacg attttccaag
     8581 atgtggtaag tgttttaacc aattatcgag acagtttaat acaacttgcc tattatcgaa
     8641 atggacaaaa agaacaggta accattcagc ttgatcaaac aggtaaattg ggtgtcgctg
     8701 ttcgtccttt taccgaattt ttggaaaccg aacacgtgaa atacggattg ctggcttcca
     8761 tacctgccgg tatttctctg ggttgggaaa cgttaaccag ttatataaaa caatttaaat
     8821 tagtatttac caaagaaggt tctaaacaat tgggtggatt cgggtcaatt gggaaattgt
     8881 ttccacaaac atggaattgg caaatttttt ggtctatgac agccctgctt tcagtcattc
     8941 tggcttttat gaactttttg cctattcctg ctttggatgg aggctatgtg ttatttcttc
     9001 tgtatgaaat atttacgggg aaaaagccaa gtgacaaatt tttagagcaa gcccaaacta
     9061 taggaatgct tctgttattg gctttgttga tttatgccaa cggaaacgac atttataaag
     9121 ccatttttaa ataaaaaaaa tagaaaaatc agggaggctg tcttaaaagt agagacagtc
     9181 tcttttttgt atgtcgggca tggtattaag cttgcaggat gcgggtttct gtatgtgccg
     9241 agttgataag aaacattagc gaatggcaag ggtgttgcgg gtgactgcaa atctgaaaga
     9301 agccatcagc aaagctgacg ttttgatgaa caaaaatctg atagaaggct ctaatgagag
     9361 ggtaaccgag caatggattg ggaacgcccg aaattcaacc gtaactcaag aagagtaaat
     9421 caggcagaac gcagtgaaag tttaatatct tatatcccga gaggtcttgt agggtgcatc
     9481 agcagtgcaa gaaatgcaac gatgtatttt acactttaca agaagtcagc aggggacata
     9541 gtattctgtt gaaaaacaga agaaggtctg
//
LOCUS       DAOSIB010000076         9043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_7282_length_9043_cov_12.087561, whole genome shotgun sequence.
ACCESSION   DAOSIB010000076 DAOSIB010000000
VERSION     DAOSIB010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9043
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7282_length_9043_cov_12.087561"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLD44_07095"
     CDS             complement(<1..410)
                     /locus_tag="PLD44_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99133.1"
                     /translation="MEKGTKSYISGNQLEVAVKTVLIGKGFELVKYRDWEKNQEKYGK
                     ELLLENVPFTTIYEHNGNTEFLLISEKYELKIRIECKWQQVGGSVDEKLPYLYLNTIE
                     AMPEETIMILIDGSGWKTGAIKWLKDAVKQKKYT"
     gene            complement(410..1399)
                     /locus_tag="PLD44_07100"
     CDS             complement(410..1399)
                     /locus_tag="PLD44_07100"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HOF99134.1"
                     /translation="MAIDTKYLTSDTIGSVVQDLFNPSNVGAKPFLKWAGGKGQLLDI
                     FQKLYPEQLKRKKIKNFYEPFLGSGAVFFDITQKYEIENAFLYDINEELILAYKVIQR
                     DVSKLIDFLHRYQKTYLKLDKVKRKEFFYNQRTNYNLQRFNIDYDKYSENWVPRAAQL
                     IFLNRTCFNGLYRVNLKGEFNSPVGDYDNPTICDEQNLLAVNKVLEIAEIKKADFKDI
                     VTDLRSDSFVYFDPPYRPISKTASFKAYHKQGFTDSEQLQLAQLFKQLDSEGSFVMLS
                     NSDPKNIDPDDNFFDDMYNGFNIVRVPARRMINSDPTKRGRINEIVVTNYTTA"
     gene            complement(1525..2412)
                     /gene="dapA"
                     /locus_tag="PLD44_07105"
     CDS             complement(1525..2412)
                     /gene="dapA"
                     /locus_tag="PLD44_07105"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOF99135.1"
                     /translation="MTINNKLTGMGVALVTPFKSDDSIDFDALGRLVEYIINNNADYL
                     VVCGTTAETPTLTDKEKDEIARFVVQCNAGRLPIVLGIGGNNTRQVVEKVKTSDLSGI
                     DAILSVTPYYNKPSQEGLYQHYSAIAKASPLPLVLYNVPGRTGVNMTAETTLRLAKEF
                     SNICAVKEASGNFTQIDDIIKNKPQDFMVISGDDGITFPLITLGAVGVISVIGNAFPR
                     EFSRMVRLALQGDYQNARQIHYRFTELFELLFVEGNPAGVKSMLAVMGMIENKLRLPL
                     VPNTIKTYEKIRSVLNQLN"
     gene            complement(2443..3021)
                     /locus_tag="PLD44_07110"
     CDS             complement(2443..3021)
                     /locus_tag="PLD44_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99136.1"
                     /translation="MKFLSRKKIKNAAIKLSDANFHIPKFLNYSDIHSILLLFEADDA
                     EKNLQVKKIVQMLKADGKKVTAIGCPRNKKLKTDFPIFENLTIVDKSQTDFFEKPRHS
                     VFKSIDANKFDAMLDLSLNENIPLQYIALYSNALLKIGVRKTSFPLYDFILDIDSIVA
                     NLKTDHLPIDLLYIFNQIIFYLKNIQSCDEKA"
     gene            complement(3095..5116)
                     /gene="ligA"
                     /locus_tag="PLD44_07115"
     CDS             complement(3095..5116)
                     /gene="ligA"
                     /locus_tag="PLD44_07115"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOF99137.1"
                     /translation="MTVKEQIEALRQEIEQHNYYYYVLSQPIISDYEYDMKMKQLQEL
                     EEKYPEYFDPLSPTQRVGSDITTSFEQAYHTYPMLSLGNTYTEGEVKDFYERVRKALN
                     EPFELVCELKYDGVSISITYEHGEIQRAVTRGDGEKGDVVTANIKTIRSIPLRLKGDY
                     PDKFEIRGEVLMPWAVFEALNKEREEQEEPLFANPRNAAAGTIKLQNSAIVASRKLDA
                     YFYYVLGEQLPFDTHFENLQAARGWGFKISENIKLCRTLDEVWEFIHFWDKERKNLPV
                     PIDGIVLKVNSLEQQRKLGTTAKSPRWAIAYKYQAEKALTKLLSVTFQVGRTGTVTPV
                     ANLEPVQLSGTTVKRASLHNADIIESLDLHIGDMVYVEKGGEIIPKITGVEKSARPQN
                     GEKVVFIKNCPECNAPLIRYPGEAAHYCPNENGCPPQIKGKIEHFVSRKAMNIEGLGE
                     ETINLLYENGLLHNVADIYDLKISDLVSLERLGLKSASNLISSIEKSKEVPFERVLFA
                     LGIRFVGETVAKKLAYAFKSIEALQQADYEDLIQVEEIGDKIAQSVRQYFSDPKNLEI
                     INRLKAAGVQLSLNDDKSKRKGNELEGKSIVISGTFKYHSREEYKDLIEAHGGKNVGS
                     VSSKTSFILAGENMGPEKLKKAQNLGIELIDENKFLSMINEEIPSSATE"
     gene            complement(5241..6098)
                     /locus_tag="PLD44_07120"
     CDS             complement(5241..6098)
                     /locus_tag="PLD44_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOF99138.1"
                     /translation="MKKLGILFSAIILLICSGFVSAQQKKTITLNVATFNLRLDTEDD
                     GENAWPNRKEFVKSLIRFHDFDIFGTQEGYKHQLDDILKLGGYAYVGVGRDDGVDEGE
                     HSAIFFKTNRFEVLDKGNFWYSKTPEVPSKGWDAFCCNRLCSWGKFKDKSSGKIFFVF
                     NSHFDHIGHIARKNSSLLLLKRIGEIAGNFPVFCTGDFNSTPDAEPIQIIAKSGKLFD
                     SYHITKQPPYGTVGTYNSFSIKSKMDERIDYIWVTSGISINKYGVLNEQQYGRFASDH
                     FPVMVSATL"
     gene            6509..7054
                     /locus_tag="PLD44_07125"
     CDS             6509..7054
                     /locus_tag="PLD44_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4924 family protein"
                     /protein_id="HOF99139.1"
                     /translation="MFIAQRLKKENICEYLLYMWQIEDLIRAFQLNIDLIDENIIAPY
                     PISETEKRTMYEWYESLIDMMRKENVQEKGHLQLNKNVIIDLNDFHELLLQSGADSEY
                     NHLFYSILPHLVQLRKKAEVNINDIELCFNFLYGIMMLNLKKEKISDETAAAQKDISK
                     FMALLAKDYKLYQEGELDLYK"
     gene            7134..8006
                     /gene="rfbA"
                     /locus_tag="PLD44_07130"
     CDS             7134..8006
                     /gene="rfbA"
                     /locus_tag="PLD44_07130"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOF99140.1"
                     /translation="MKGIVLAGGSGTRLYPITKSISKQIIPVYDKPMIYYPLSILMLA
                     GIREILIISTPQDIHLYERLLGDGSDFGIAIEYAVQPSPDGLAQAFIIGENFIGNDNV
                     CMILGDNIFYGFDFSRTLIEAANLKDGAIVFGYYVNDPERYGVAEFDAEGKVLSLEEK
                     PKQPKSNYAVTGLYFYSNDVVEKSKHLKPSSRGELEITDLNRLYLNEGRLNMKILGRG
                     MAWLDTGTHDSLLDASNFIATIENRQGLKIACLEEIAYRNGYIDREQLLRLAEPLKKS
                     HYGEYLIKIAGEVK"
     gene            8421..8657
                     /gene="rpmB"
                     /locus_tag="PLD44_07135"
     CDS             8421..8657
                     /gene="rpmB"
                     /locus_tag="PLD44_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOF99141.1"
                     /translation="MPKICQITGKKSLKGNNVSHSKRRTKRNFDVNLFTKKFYWVEED
                     TWISLDISAAGLRTINKLGLDASIKKALKKGYLK"
     gene            8680..8868
                     /gene="rpmG"
                     /locus_tag="PLD44_07140"
     CDS             8680..8868
                     /gene="rpmG"
                     /locus_tag="PLD44_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOF99142.1"
                     /translation="MAKKTKEARVQVILECTEHKDSGMPGTSRYVTQKNRKNTPERLE
                     LRKYNPILKRVTIHREIK"
     gene            8873..9025
                     /locus_tag="PLD44_07145"
     CDS             8873..9025
                     /locus_tag="PLD44_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HOF99143.1"
                     /translation="MAKKVVASVQKGEGRTFSKVIKMVKSPKTGAYMFTEEMVPNDKV
                     KDFLNK"
BASE COUNT         2898 a         1644 c         1556 g         2945 t
ORIGIN      
        1 cttgtatatt ttttctgttt aacggcatcc tttaaccatt taattgcgcc tgttttccat
       61 ccagagccat ctattaatat cataattgtt tcttctggca ttgcttcaat tgtgtttaaa
      121 tacaggtacg ggagtttctc atcaactgaa ccaccaactt gttgccattt gcattctata
      181 cgaattttta attcatattt ttcagaaata agcagaaatt ctgtatttcc gttgtgctca
      241 taaattgttg taaaaggaac attttcaagt aataattcct taccatattt ttcttgattt
      301 ttctcccaat ctctatattt tacaagctca aatccctttc ctatcaaaac cgttttaacg
      361 gcaacttcaa gttgattgcc tgatatataa cttttagttc ctttttccat tatgctgttg
      421 tgtaatttgt taccactatt tcatttattc tgcccctttt ggttgggtcc gaatttatca
      481 ttcttcttgc aggaactctt actatattga atccattata catgtcatcg aaaaaattat
      541 catcaggatc aatattttta gggtctgaat tactcaacat gacaaaagat ccttctgaat
      601 caagttgttt aaaaagctgt gctaactgca attgttcact atcagtaaaa ccttgtttgt
      661 gatatgcttt aaaacttgca gttttactta tcggcctata aggtggatcg aaataaacaa
      721 aagaatcaga tcttaagtct gttacaatat ctttaaagtc agcttttttt atttcggcaa
      781 tttctaaaac cttattgact gccaataaat tttgttcatc acaaattgtc gggttatcat
      841 aatcgcctac cggtgagtta aattctcctt ttaaattcac cctgtaaagt ccgttaaaac
      901 atgttctgtt taaaaaaatt agttgtgcag cacgaggaac ccaattttca gaatatttat
      961 cataatcaat attaaacctt tgtaaattat aatttgtacg ttgattgtaa aaaaattctt
     1021 tacgttttac tttatcaagt ttcaaataag ttttttggta acgatgtaaa aaatcaatta
     1081 gtttgctaac atccctttga attactttat atgccagaat tagttcttca ttaatatcat
     1141 aaagaaaagc attttcaatt tcatattttt gagttatgtc aaaaaaaact gccccacttc
     1201 ctaaaaatgg ctcataaaaa ttcttgatct tttttctctt aagttgttca ggataaagtt
     1261 tctgaaaaat atccaaaagt tgtcctttgc caccagccca ctttaaaaat ggttttgcgc
     1321 caacattaga tggattaaat aaatcttgca caacagagcc aattgtgtca gaagttaaat
     1381 attttgtatc aattgccata attctatttg aatgtccaaa tttacaaaaa tattttttgt
     1441 gttacttcat tctaatgatg tagcattttt tagcatgaca actttcgtcc tgtcggggaa
     1501 aattttttgt aattttgtaa tttttcaatt taactgattg agtaccgagc gaattttttc
     1561 gtacgtttta atggtattgg gcaccagagg aagacgcaat ttgttttcta tcattcccat
     1621 gacagccaac atgcttttta cgccggcagg atttccttcc acaaaaagca attcaaaaag
     1681 ttcggtgaaa cgataatgta tctgacgtgc attttgataa tcgccctgca gagccaaacg
     1741 aaccatacgg ctgaattcgc gcggaaaagc attgccgata acggaaatca caccgacagc
     1801 acccaacgta atcaacggaa aagtaatgcc gtcgtcgccg gaaataacca tgaaatcctg
     1861 aggcttgttt ttgataatat cgtcgatctg ggtaaaattt ccggaggctt ccttcactgc
     1921 gcaaatattg gaaaattctt ttgccaagcg taaagtagtt tcggcagtca tgtttacgcc
     1981 tgttcttccc ggcacattgt agagcacaag tggcaacgga gaagctttgg ctatggccga
     2041 ataatgttga taaagtcctt cctgagaagg tttgttgtaa tacggcgtaa ccgataaaat
     2101 ggcatcaatc cccgataaat cggaagtttt cactttttcg accacttgtc tggtattgtt
     2161 tccgccaatt cccaatacaa taggcaatct tccggcattg cactgaacaa caaaacgggc
     2221 tatttcatcc ttttccttgt ctgtcaaggt aggtgtttcg gctgtagtgc cacatacaac
     2281 caggtaatcg gcattgttgt tgattatgta ttcaaccaac cttcccaacg catcaaaatc
     2341 tatcgaatcg tcgcttttaa aaggtgttac taaagctaca cccatccctg tgagcttgtt
     2401 gttaatcgtc atggagtgaa aaaattttat tctgttacaa aattacgctt tttcatcgca
     2461 actttgtata tttttcaggt aaaaaattat ctgattaaaa atatataaca aatcgatggg
     2521 caaatggtca gtctttaagt ttgcaacaat gctgtcgata tccaatataa aatcatacaa
     2581 tggaaacgat gtttttctaa ctccaatttt caacaatgca ttggaataca aagcaatata
     2641 ttgaagagga atattttcgt tcaaggataa atcgagcatt gcatcaaatt tgttggcgtc
     2701 gattgattta aagacagaat gtctgggttt ttcaaaaaaa tcggtctgac ttttatccac
     2761 aatcgttaaa ttttcaaaaa tcgggaaatc ggttttcaat ttcttatttc gaggacaacc
     2821 aatagctgta actttcttcc catctgcttt cagcatctgc actatttttt tcacctgaag
     2881 atttttttcc gcatcatcgg cttcaaacaa cagcaaaata gaatgaatgt ctgaataatt
     2941 tagaaatttg ggtatatgaa aattggcatc ggacaatttg atagctgcat ttttaatttt
     3001 ttttctgctc aaaaatttca ttgtaatctc tttaaatagg ttgttttggg ttgacctgaa
     3061 aaaacacaga aattgaactg ttttataatt ttattcattc agtggctgat gatggaattt
     3121 cttcatttat catcgaaaga aatttatttt catcaattag ttcaatgccc aaattttgtg
     3181 cctttttcag tttttccggt cccatgtttt cacctgccag aataaaagaa gtttttgaag
     3241 acacgctgcc aacatttttt ccgccatgtg cctcgattaa atctttgtat tcctcacgtg
     3301 aatggtattt gaatgtaccg ctgatgacga ttgattttcc ttcgagctcg tttccctttc
     3361 gtttggattt atcatcatta agagacagtt gaactccggc agcttttaag cggttaataa
     3421 tttccagatt tttcggatcg gaaaaatatt gtcggacact ttgagctatt ttatctccta
     3481 tttcctccac ctgaatcaaa tcttcataat cggcttgttg caaagcttcg attgatttaa
     3541 aggcataagc cagctttttg gcaaccgttt cgccaacaaa acgaattccc agcgcaaaca
     3601 atacgcgttc gaaaggaact tccttcgatt tttctattga actgatcaga ttagaagccg
     3661 atttcaaacc caatcgttcc aatgaaacca aatcggaaat tttcagatcg taaatatcgg
     3721 ccacattatg aagcaaaccg ttttcgtaaa gcaaattaat agtttcttca cctaaacctt
     3781 cgatgttcat ggcttttctg ctcacaaaat gttcaatttt tcccttaatc tgaggcgggc
     3841 aaccgttttc gttgggacaa tagtgggcag cttcgccggg atatcgaatg agaggagcat
     3901 tgcattcggg acaatttttt ataaaaacaa ctttttcgcc gttttgtggc cgtgcgcttt
     3961 tttccacacc ggtaattttc ggaataattt ctccaccttt ttcgacataa accatatcgc
     4021 caatatgcaa atcgagcgat tcaataatat ctgcattatg caacgaagcg cgtttcactg
     4081 tagttcccga aagttgaacc ggctcaagat tggctacggg tgttacggtg cctgttcttc
     4141 caacctgaaa cgtaaccgaa agaagtttgg tcaatgcttt ttcggcctgg tatttataag
     4201 caatggccca acgtggcgat tttgcagttg tgccaagttt tcgttgttgt tccaatgaat
     4261 tgactttcaa tacaatgcca tcaatcggaa ccggtaaatt ttttcgttct ttgtcccaaa
     4321 aatgaataaa ttcccacact tcgtcgagtg ttctgcaaag tttaatattt tcagaaattt
     4381 taaaacccca accacgagct gcctgcaaat tttcaaagtg cgtgtcaaaa ggaagttgtt
     4441 cgcccaatac ataataaaaa taggcatcca gcttacggga agcaacgatg gcagaatttt
     4501 gaagtttaat ggttcccgca gcagcgttgc gaggattggc aaaaagcggt tcttcctgtt
     4561 cttctctttc cttattgagt gcttcaaaaa ctgcccacgg catcaacact tcgccgcgaa
     4621 tttcaaattt atcgggataa tcgcctttca atcgcaaggg aatgctccgg atagttttga
     4681 tgttggctgt tactacatca cctttttctc cgtctccacg cgttacagcc cgttgaattt
     4741 caccatgttc gtatgtaatg gaaatggaaa ccccatcata tttcagttca cataccagtt
     4801 caaaaggttc atttaatgct ttccgaacac gttcataaaa atctttcact tcaccttctg
     4861 tataagtatt tcccaacgaa agcatgggat aggtatgata agcctgctca aaactggttg
     4921 taatatcgct gccaacacgc tgggtgggcg aaagaggatc aaaatattca ggatattttt
     4981 cttccaattc ctgcaactgc ttcattttca tatcatattc gtaatcggaa ataatgggtt
     5041 gcgaaagcac gtaataataa tagttgtgtt gctctatttc ctgccgcagc gcttcaattt
     5101 gttccttgac tgtcataaat tagaaaaata ataattcctg atgggcaaaa ttaatcaatt
     5161 aaagaaaagt gaacaagaaa agggcggaaa ttggaagaaa gtcgcaaaat tcccgccctc
     5221 aaaaaaataa aattaaaatt tcaaagagta gcgctgacca ttaccgggaa atgatcggaa
     5281 gcaaagcgcc cgtattgctg ttcgttaagt accccatatt tattgatgga aataccgctt
     5341 gtaacccaaa tataatcaat tctttcgtcc attttggatt ttattgaaaa tgaattatat
     5401 gtacccaccg ttccatatgg aggttgtttg gtaatatgat aactatcgaa aagttttccg
     5461 cttttagcaa taatttgaat tggctcggca tcgggtgtag aattaaaatc gcccgtacag
     5521 aaaaccggaa agtttcctgc tatttcacca atacgcttca aaagcagtaa ggatgaattt
     5581 tttcgtgcta tgtgcccaat atgatcgaaa tgagaattaa aaacaaaaaa tatttttcct
     5641 gaagatttgt ctttaaattt tccccacgaa caaagacgat tgcaacagaa ggcatcccaa
     5701 ccttttgaag gaacttcagg cgttttggaa taccaaaagt ttcccttatc caatacttca
     5761 aagcgatttg ttttgaaaaa aatagcagaa tgttctcctt catcaacgcc atcgtctcgt
     5821 cctacgccaa cataagcata tccacccaat tttaaaatgt cgtccaattg atgtttataa
     5881 ccttcctgtg ttccaaaaat gtcgaagtcg tggaaacgaa tcagactttt gacaaattct
     5941 ttccgattgg gccatgcgtt ttcaccatca tcttccgtat cgagccgaag attaaaagtt
     6001 gcaacgttga gcgtaatggt ttttttctgc tgtgccgata caaagccgga gcaaattaaa
     6061 agaataattg cagaaaacag aatgccgagt tttttcataa gattgaaatt taggtttttt
     6121 atagcttaag aaaattataa tttgaattga aatttccttt ttaaatcttt ataaactact
     6181 gatttatact gtttgatgaa aattaattac tcacacaaaa tattgtaata ttagtaatca
     6241 actacaagat tactaatatt ttttctaatt tagatttttt tagttgattt tttatatttt
     6301 attttcatgt aacaactcag ctaatttttt aaattcagaa cataaaaaac taatggcaaa
     6361 gcaattaatt tttaatgaga attcaacacg aaagactgag taaaattccc gatatttata
     6421 ttaattttgc aaaatagttc agtaagagac acaaattcct attcttaaaa atattggcat
     6481 tatattcgta ccatcaaaat ctttcatcat gttcatagct caaagattga aaaaggaaaa
     6541 tatctgcgaa tacttgcttt acatgtggca aatcgaagac ctgattcggg cttttcagct
     6601 gaacatcgac ttaatagatg aaaatataat tgctccgtac ccgatttctg aaacagaaaa
     6661 aagaaccatg tacgaatggt atgaaagtct gatcgacatg atgcgcaaag aaaacgttca
     6721 ggaaaaaggt catttgcaat tgaataaaaa tgtaattatc gacctgaacg atttccatga
     6781 acttttgttg caatcgggcg ccgattcgga atacaatcat ttattttaca gtattttacc
     6841 ccatcttgtt cagttgagaa aaaaagcgga agtaaacata aacgacatag agttatgttt
     6901 taattttttg tacggtatca tgatgttgaa tttgaaaaag gaaaaaattt cagatgaaac
     6961 ggccgctgcg caaaaggata tttcaaagtt catggcattg ctggcaaaag attataagct
     7021 ttatcaggaa ggagaactgg atttgtacaa ataaaaaact tttgagaatt cgcttacatt
     7081 tttatcgttt aaaataattt cgcagaaaaa aacaatttaa aaaaatcttc atcatgaaag
     7141 gcattgtttt ggcaggtggt tccggtactc gactttatcc cataaccaaa agcatttcca
     7201 aacaaattat tccggtttac gacaaaccca tgatttatta tccgctctca atacttatgt
     7261 tggcgggcat tcgtgaaatt ctcattattt ccacaccgca ggatattcat ctttacgaac
     7321 gtctgttggg tgatggttct gatttcggta ttgcaattga atatgcagtg cagccatcgc
     7381 cggatggttt ggctcaggcc tttattattg gtgaaaattt cattggaaac gacaatgttt
     7441 gcatgattct tggcgacaat attttttatg gattcgactt ttccagaaca ttgatcgaag
     7501 cggccaacct aaaagacgga gccattgttt tcggttatta tgtcaacgat ccggaacgat
     7561 atggcgtagc cgaatttgat gctgaaggca aggtattgag tttggaagaa aaaccaaaac
     7621 agcccaaatc aaattatgcc gttacaggac tttatttcta tagtaatgat gtagttgaaa
     7681 aatcgaaaca cctgaaacct tcatcgcgtg gagaattgga aattaccgat ttaaacagac
     7741 tttacctgaa cgaaggacga ttgaatatga aaattttggg tagaggaatg gcctggctcg
     7801 atacaggaac acatgacagt ttgctggatg cttccaactt tattgccacc atagaaaacc
     7861 gtcaggggct gaaaatagcc tgcctcgaag aaattgccta tcgcaacggg tatatcgacc
     7921 gtgagcaatt gctacgactg gccgaaccat tgaaaaaaag tcattatggc gaatatttga
     7981 ttaaaattgc aggtgaagtc aaatagtttg attctaaaac tatttttggc tacaaaagtg
     8041 agagagtctc ttttattaag cataagacgc ttttaaatat attttcaaaa cttaaatgat
     8101 acacttttct tgaatgtttt atcctttgaa aatataattt gtttgctggt tttatacata
     8161 actgaaactt gcataaacct gtcgctttcc tgaaagatat tttattgatt tactatggtt
     8221 tatgtaaatt tttcagaccc gattaaaaat ggattttctg taaagaaaaa acaattaaca
     8281 aattgattaa caaacatttt aatgagttta taaagataaa tttcaaaaaa tagggataaa
     8341 attgcttgta tatgtaaaaa ataaaatgta attttgcaac tcaattttga gcagaagtat
     8401 aacaacataa atgaaataaa atgcctaaga tttgtcagat tacaggtaaa aaatctttaa
     8461 aaggaaataa tgtttcacac tcgaaaagac ggacaaaaag aaatttcgat gtaaacttat
     8521 ttaccaaaaa gttttattgg gtggaagagg acacatggat tagtcttgat atttcggctg
     8581 ccggtttaag aaccatcaac aagttgggat tggacgcatc cattaaaaaa gcgctgaaaa
     8641 aaggatattt aaaataaata ctattaggag gcatacatca tggcaaaaaa aacgaaagaa
     8701 gcaagggtgc aagttattct ggagtgtacc gaacataagg acagtggtat gcccggaact
     8761 tcgcgctatg ttactcaaaa gaacagaaaa aatactcccg aaaggcttga gctcaggaaa
     8821 tacaatccaa tattaaaaag agttactatt cacagagaaa taaaataaga ttatggcaaa
     8881 gaaggttgtt gcatcagtac aaaaaggtga aggccgtact ttttcaaaag tgataaaaat
     8941 ggtcaaatca cctaaaacag gggcttacat gtttactgaa gaaatggtgc ccaatgataa
     9001 agtaaaagat tttctcaata aatgaaaaaa taaattattt cct
//
LOCUS       DAOSIB010000077         8928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_7392_length_8928_cov_12.547504, whole genome shotgun sequence.
ACCESSION   DAOSIB010000077 DAOSIB010000000
VERSION     DAOSIB010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8928
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7392_length_8928_cov_12.547504"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..997)
                     /locus_tag="PLD44_07150"
     CDS             complement(11..997)
                     /locus_tag="PLD44_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HOF99144.1"
                     /translation="MKEDNSLYPLKFQPILKEKIWGGTRLKKLFNKQTESDKIGESWE
                     LSGFEGDESIVSNGFLAGNNLTELIEIYMGDLVGDKIFEEFGLTFPLLFKLIDANDDL
                     SIQVHPDDETAAQRHNSLGKTEMWYVIDAEPGGELIVGFINDCSREEYLKALEEGRVA
                     DLLKRVPVSKGDVIYIPAGLVHAIKKGVLVAEIQETSDLTYRIFDYKRTDEHEKEREL
                     HTELALDVINFSATKNPKVEYESKVNAVTTLVSCDYFTTNLLIFNRNIIRNYSSLDSF
                     VVYMCLEGNFILECENHKILVEKGETVLIPASLDEVSLIPQGEVKLLEVYIQ"
     gene            complement(1020..2060)
                     /gene="galE"
                     /locus_tag="PLD44_07155"
     CDS             complement(1020..2060)
                     /gene="galE"
                     /locus_tag="PLD44_07155"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOF99145.1"
                     /translation="MMSKVLVTGGTGYIGSHTVVELQQEGHEVIIVDNLFNSDIEVLN
                     GIEKITGIRPIFENIDCTDYVGMDRMFERHKNIDAIIHFAAYKAVGESVEKPLLYYRN
                     NILSLINLLQLMPVHKIKHLVFSSSCTVYGQPDILPVTENSPFKPALSPYGNTKQICE
                     EIIRDTIHSNSFYHSIVLRYFNPIGAHPSAEIGELPNGVPNNLLPFITQTAIGIRKQL
                     LVYGNDYNTPDGTCIRDYIDVVDLAKAHVSALNRMLNHQQKNNLEVFNLGTGRGLSVL
                     EIIKMFEKVNGVKVPYKIVGRREGDIEKVWADPSYANKELGWKAQRPLEETLRSAWEW
                     EKKLAERTKQNA"
     gene            complement(2067..2972)
                     /locus_tag="PLD44_07160"
     CDS             complement(2067..2972)
                     /locus_tag="PLD44_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOF99146.1"
                     /translation="MKPTLLILAAGMGSRYGGLKQLDGLGPNGETIMDYSIYDAIQAG
                     FGKVVFVIRKSFEKEFNELIVSKFKNLIDVELVFQELDKVPEGCTYSPEREKPWGTNH
                     ALLMAKDVIHEPFGVINADDFYGRESYFILADFLRSVENKKNEYCMIGYRVGNTLSES
                     GPVSRGICVADENNFLKDVVERTHIVEKGGTIIYLDENGKEVAIHPNVPVSMNMWGFT
                     PDYFDYSWEAFKKFLAENGQNVKAEFYIPTVVNQLIHENKVTCKILDTPSKWFGVTYS
                     NDRPQTVLKINELIRKGVYPEKLFK"
     gene            3316..4134
                     /gene="ispE"
                     /locus_tag="PLD44_07165"
     CDS             3316..4134
                     /gene="ispE"
                     /locus_tag="PLD44_07165"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOF99147.1"
                     /translation="MIVHPNAKINIGLNITGLLPNGYHSIETVFYPVNVYDHLKIEPI
                     PKAAENQFVVKGLSIDGKPENNLVIKAWQLLRDEYKIAPVKIFLEKNIPMGAGLGGGS
                     SDAAFMLKTLNDLFQLDIDREKLKHLAAKLGADCPFFIDNQPVFAQGKGDKFHPLKLS
                     LKNFYLVLVKPDIHIATANAYAETVPKKPEFPLQELVKKPIEEWKYFIKNDFEESIFK
                     RYPLIAEIKQKLYETGAVYASMSGSGSAVYGIFYDRPVRNGYFDNYFFSIVPLS"
     gene            4194..4631
                     /locus_tag="PLD44_07170"
     CDS             4194..4631
                     /locus_tag="PLD44_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOF99148.1"
                     /translation="METTNKIQKIIGNQETFTNIGLLIFRLIIGIFMLTHGLQKITNF
                     EMLKAFFPDPLGIGASTSLILIILAEVGCSLLIIFGIFTRLAAIPPMFGMAVAAFMVH
                     ANDPFSVKEIAFLYLFLYVALLFLGAGKYSIDYLLTKHLTKKK"
     gene            4912..7449
                     /gene="gyrA"
                     /locus_tag="PLD44_07175"
     CDS             4912..7449
                     /gene="gyrA"
                     /locus_tag="PLD44_07175"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOF99149.1"
                     /translation="MIEQERIIKVNIDEEMKSSYIDYSMSVIVSRALPDVRDGFKPVH
                     RRVLFGMNELGNTPDKPYKKCARIVGEVMGKYHPHGDSSIYGTLVRLAQPWSMRYPLV
                     DGQGNFGSVDGDSPAAMRYTEARLQKIAEEMLADIDKDTVDFQPNFDETLKEPVVLPS
                     RLPNLIVNGSSGIAVGMATNMAPHNLSETVDAIVAYIDNNDISIQELTKFIKAPDFPT
                     GGIIYGYAGVKEAFETGRGRIVIRSKVEIETEASGREKIVVKEIPYQVNKVELIKTIA
                     NLVEEKKIEGISHINDESDREGMRIVIEVKRDANSNVVLNKLYKLTALQSTFNVNNIA
                     LVHGRPHMLNIKDLIHYFVEHRHDVVIRRTKFELAEAEKRAHLLEGFMIILDNLDEAI
                     QIIRNSKTPDEARSNLMSRFNLTEIQARAIVEMRLRQLTGMEQEKLRAEYQEILALIA
                     HLKEILDKIELRMQIIKDELLEIKAKYGDNRRTEIVYSSEEFNPEDFYSNDEMIITIS
                     HLGYIKRTPLSEFRTQGRGGIGSKGSDSRDEDFIEHIYPASMHNTMLFFTQKGKCYWL
                     KVYEIPEGSKSSKGRAIQNLLNIDGDDKVTAFIRVTGLTDPDYNSSHYLVFCTKKGTI
                     KKTSLEAYSHPRQNGVTAINIREDDEVIQVRLTDGNSEILMANRNGRAIRFHESTVPE
                     MGRVSTGVRGMKLDDEKDEVVGMICVNKEDNTNILVVSEQGYGKRTLLDDENGEPIYR
                     ITNRGGKGVKTMNITEKTGKLVAIKNVTDDNDLMIINKSGIAIRLKVADIPVIGRATQ
                     GVRLINLEKRNDEIASVCKVDSEPVEEIVDEETIETELAENNEPNEN"
     gene            7619..8848
                     /locus_tag="PLD44_07180"
     CDS             7619..8848
                     /locus_tag="PLD44_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOF99150.1"
                     /translation="MKKIILSFFLVLSFTFTYAQKSNVSKAKNKALMENPDFAGAREA
                     IQAALNDSITKNDPNTWYVAGLIGYKENDYWYQKLILNQKYDADAKGQAILESYNYFK
                     KAAELDQLPDAKGKVKPKFLKDIKTKIKEYYTLQPNLIGYGAYLYEKKDYEGALKVFE
                     TYLEIPTLPYLKDEIVIDSTYYMIQYFAALSATNAGYHDKAISLYEDLKHKNYETLNV
                     YELLADEYRTINDTIKYVETLKEGLEKFPSELWFLQNLINHYIYSGKIQDALAYLNDA
                     IKRDPNIAQYYFVKGNLEENLGNYDEAISAFNKAIELDPNLAEAYAGIGRLYYNKAIK
                     MLEVANDIKDVKLFNQEKEKANKVFKESIPYFQKAKELNPNEIEYKRTLRTLYYRLQM
                     DKEFEAIDEEIRAMENQ"
BASE COUNT         2933 a         1736 c         1557 g         2702 t
ORIGIN      
        1 gaaataatcc ttactgaata tatacttcca gcaattttac ctctccttgc ggaatcaatg
       61 aaacttcgtc caaactggca ggaattaaaa ctgtttcgcc cttttccacc aagattttat
      121 ggttttcgca ttctaaaatg aaatttcctt ccaaacacat ataaactaca aaggaatcca
      181 gcgaactgta attgcggata atattacgat taaatattag cagattggtg gtaaaataat
      241 cacacgaaac cagtgttgtt accgcattaa ctttagattc gtattcaact tttggatttt
      301 ttgtggcgga aaaattaatg acatccaaag ccaattctgt atgcagctca cgctcttttt
      361 catgttcatc ggttcgtttg tagtcaaaaa tacgataagt aagatcactt gtctcctgaa
      421 tttctgcaac caatacgcct ttttttatgg cgtgtaccaa acctgccgga atataaatca
      481 catcaccctt gctgaccgga actcttttca ataaatcggc cacacgtcct tcttccaaag
      541 ctttcaaata ttcttcgcgt gagcaatcgt ttataaatcc aactatcaat tctcctcccg
      601 gctcagcatc tattacatac cacatttcgg tttttcccaa cgaattgtgc cgctgagctg
      661 ccgtctcatc gtcgggatgc acctgaatgg acaaatcgtc attggcatca ataagtttaa
      721 aaagcaaggg aaaagtcaac ccaaattctt caaaaatctt atccccaacc aagtctccca
      781 tatatatttc aatcaattcc gtcaaattat tcccggcaag gaaaccattc gatacaattg
      841 attcatcacc ttcaaaaccg gaaagttccc aactttcacc aatcttgtcg gattcggttt
      901 gcttgttgaa aagttttttt agccgcgttc ctccccaaat tttttctttt aaaatgggtt
      961 gaaattttaa gggatataag ctattgtctt ctttcatttt aattgaatga aattttttat
     1021 caagcatttt gtttggtacg ttcagccagt tttttctccc attcccatgc actgcgaagc
     1081 gtttcttcca aaggtcgctg agctttccaa cccaattcct tattggcata actcggatcg
     1141 gcccaaactt tttctatatc accttcccgt cttcccacaa ttttataagg taccttgacc
     1201 ccattaactt tctcaaacat tttgataatt tccagcaccg aaagtcctct gcccgttcca
     1261 agattgaaaa cttccagatt gtttttctgc tgatgattca acatgcggtt gagagccgaa
     1321 acatgtgctt ttgccaaatc aacgacatcg atgtagtcgc gaatacatgt tccatcaggc
     1381 gtattataat cgtttccata aaccagcaac tgtttcctga ttccgatagc tgtttgagta
     1441 ataaacggga gcaaattatt gggcacgcca ttaggcaatt caccaatttc ggccgaagga
     1501 tgcgctccaa tcggattgaa atatcgaaga acaatgctat gatagaatga attggaatga
     1561 atggtatcgc gaataatttc ttcacatatt tgtttggtat tgccataagg agaaagtgcc
     1621 ggcttaaaag gtgaattttc cgttaccggc aaaatatccg gctgtccata aacggtgcac
     1681 gaagacgaaa aaaccaaatg cttgatttta tgtaccggca tcagttgcaa taaattgatc
     1741 agagaaagaa tattattcct gtaatacagc aaaggctttt caaccgactc gcctacagct
     1801 ttatacgctg caaaatgaat tatggcgtct atattcttat gtctttcaaa catgcgatcc
     1861 atgccaacat aatccgtaca gtcgatattt tcgaatatgg gacgaatacc ggtaattttt
     1921 tcgattccgt ttaaaacctc aatatcggaa ttaaaaagat tatccacaat aataacttca
     1981 tgtccttcct gttgcaattc aaccacagta tgcgaaccaa tatatccggt tccgccggta
     2041 accaaaactt tactcatgat gtctgtttat ttgaataatt tttctggata tactccttta
     2101 cggattaatt cattgatttt taaaactgtc tgcggtctgt cattggaata tgttacacca
     2161 aaccacttgg aaggcgtatc caaaattttg caggtaactt tattttcgtg tatcaattga
     2221 ttgacaaccg tcgggatata aaattcggct ttaacatttt gaccattttc agctaaaaat
     2281 ttcttgaaag cttcccaaga atagtcgaaa taatcgggag taaaacccca catattcatt
     2341 gatacgggta cattgggatg aatggcaact tcctttccgt tttcatccaa ataaattatt
     2401 gtacctccct tttccacaat atgcgttctt tccaccacgt cctttaaaaa attgttttcg
     2461 tcagccacac atattcctct gctcaccggg ccactttccg aaagcgtgtt acctactcga
     2521 taaccaatca tgcaatattc attcttttta ttttctacgc tgcgcagaaa atcggccaaa
     2581 ataaaatacg actcacgacc ataaaaatca tcggcgttga ttacgccaaa aggttcgtga
     2641 ataacgtcct ttgccatcag cagtgcatga tttgttcccc aaggtttttc gcgttcggga
     2701 gaataagtac atccttctgg cactttgtcg agttcctgaa ataccaactc aacatcaatt
     2761 aaattcttga atttactgac aatcaattca ttaaattcct tttcaaaact ttttcgaatc
     2821 acgaacacta cctttccaaa tcctgcctgt atcgcatcat aaatggaata atccataatg
     2881 gtttctccat tggggccaag cccatccaat tgcttcaatc cgccataacg gctgcccatt
     2941 ccggctgcta aaattaataa agttggtttc atattttatt ttctgttgtt tatttcaaag
     3001 atttaatata gtttacagat aatcaatgtt ttcagaaaaa atttattagg aaaaattcga
     3061 gtttgcgcta aaagttttag gaaatgcaaa tttactatta ttgtttcaat taagaaaact
     3121 gtaaactact tcataaactt aactttgaca aaagtcgaaa ttttataatt tcgttaaaat
     3181 ttgctattta caacttcaaa caacaataaa aaacagtatg agattatttg aacaaaaatt
     3241 ttttgtcata gaaaaaattg aaatcaaaaa atttactact tttacaaccc cacaaataaa
     3301 attttaccga ttcttatgat tgtccatccc aatgccaaaa tcaatatagg cctaaacatt
     3361 acaggcctgc tgccaaatgg ctatcactcc atcgaaacag ttttttatcc agtcaatgtt
     3421 tatgaccatc tgaaaattga acctattccg aaagctgccg aaaatcaatt tgttgtaaag
     3481 ggtttgtcca ttgacggcaa accggaaaac aatctggtta taaaagcatg gcaactactg
     3541 cgagatgaat acaaaatagc gccggtaaaa atttttctgg aaaaaaatat tcctatgggt
     3601 gccggcttgg gcggtggttc ttccgatgct gcatttatgc tgaaaacact caacgacctt
     3661 tttcaactcg atatcgacag agaaaaatta aaacatttgg ctgccaaact tggcgccgat
     3721 tgtccttttt ttatcgacaa tcagccggtt tttgctcagg gaaaaggaga taaatttcat
     3781 ccgttgaaac tttcgttgaa aaacttttat ttggtgctgg taaaaccaga tattcacatc
     3841 gctactgcca acgcttatgc cgaaacggtg ccaaaaaaac cggaatttcc cttgcaggaa
     3901 ctggtgaaaa aacccattga agaatggaaa tattttatca aaaacgattt cgaagaatcg
     3961 atttttaaac gttatccgct gatagccgaa ataaaacaaa aactctacga aacaggcgcc
     4021 gtttatgctt ccatgtcagg ttctggatcg gccgtttacg gcatttttta cgatcggcct
     4081 gtccgcaatg gctatttcga taattatttc ttttcgatag ttccactgag ctaaagaaaa
     4141 acattttctt aacttgtttg ttgttactgg taaacaattt aattaattca aatatggaaa
     4201 ccacaaacaa aatacaaaaa attattggta atcaggaaac tttcaccaat atcggcttat
     4261 tgattttcag gctgataatt ggcatattca tgttgacaca tggcttgcaa aaaataacta
     4321 attttgaaat gctgaaagct tttttccccg atcctttggg aattggagct tctacttcgc
     4381 tgatattaat cattttggct gaagtaggtt gttccctgtt aatcattttt ggcattttta
     4441 cccgattagc tgccattcct cccatgtttg gcatggccgt agctgcattt atggttcatg
     4501 ccaacgatcc gttttccgtc aaagaaatcg cgtttcttta cctttttctt tatgttgcat
     4561 tgttattcct cggtgcagga aaatattcga tagattatct tctcacaaaa catctgacga
     4621 agaaaaaata aatcggattc attttcattt ctttcagtcc tattatgacg gaaacatttt
     4681 caaaatggtg ttttcgtctt ttttattcct cagctctttt ttattaacgt tagcaaactg
     4741 attaacaact attcgttaaa aaattcaaaa attttccaaa atgaaattta atcctcattt
     4801 tcaattaaat atctatcaat aacataaaaa ataatcgtaa aaaatcttgt ccaattgagt
     4861 aaaaagtagt aatttagagc aatttttcga taaaaataaa aaattgcata gatgattgaa
     4921 caagaaagaa ttattaaagt gaacattgac gaagaaatga aatcttcgta cattgattat
     4981 tccatgtcgg taattgtatc cagagcttta cctgatgttc gcgatggttt taagccggta
     5041 caccggcgtg tgcttttcgg aatgaacgaa ttgggcaaca ctcccgataa accttataaa
     5101 aaatgtgcaa gaattgtggg tgaagtgatg ggaaaatatc atccgcacgg cgattcttcg
     5161 atttatggaa cattggtccg actggctcaa ccttggtcaa tgcgttatcc actggtagat
     5221 ggtcagggaa attttggttc ggtagatggt gatagtcccg ctgccatgcg ttacacagaa
     5281 gcccgtctgc aaaaaattgc cgaagaaatg ctggccgata ttgataaaga cactgttgat
     5341 tttcagccaa attttgacga aactttaaaa gaaccggtgg ttcttccctc acgtttaccc
     5401 aacttaattg tcaacggttc ttcggggatt gccgtcggta tggctaccaa catggcgcct
     5461 cacaatttat cggaaaccgt agatgccata gttgcctata tcgacaacaa cgatatttcc
     5521 attcaggaat tgacaaaatt tatcaaagct cccgatttcc ctaccggcgg aattatttat
     5581 ggttatgccg gagtaaagga agcttttgaa accggacgag gccgcatcgt catccgctcc
     5641 aaagttgaaa tagaaacaga agcctctgga cgtgaaaaaa ttgtggttaa agaaatacct
     5701 tatcaggtta acaaggtgga attgataaaa accattgcca atctggtaga agaaaaaaag
     5761 attgaaggaa tttcacatat caacgacgaa tccgaccgtg aaggaatgcg catcgttatt
     5821 gaagttaaac gcgatgccaa ttccaatgtg gtgcttaaca aactttacaa gctgacagcc
     5881 ttacaatcaa ctttcaatgt caacaacatt gctttggttc atggcagacc gcacatgttg
     5941 aatatcaagg atttgattca ctactttgtt gaacatcggc acgatgttgt tattcgtcga
     6001 accaaatttg aactggccga agccgaaaaa agagcacacc tactcgaagg attcatgatt
     6061 atcctcgata atctggacga agccattcaa ataatccgca attctaaaac tcctgacgaa
     6121 gcacgctcca atttaatgag ccgcttcaac cttaccgaaa ttcaagctcg cgctattgta
     6181 gaaatgagac tgcgtcaact taccgggatg gaacaggaaa aacttcgtgc cgaatatcag
     6241 gaaatccttg cattgattgc tcatttgaag gaaattctcg ataaaataga acttcgtatg
     6301 caaatcataa aggatgaact gttggaaata aaagcaaaat atggtgataa ccgtcgtacc
     6361 gaaattgttt attcttcaga agaattcaat ccggaagatt tctattcgaa tgatgaaatg
     6421 attattacta tttctcattt gggatacatc aaaagaactc cacttagcga attcaggaca
     6481 cagggacgtg gcggcatcgg ttcaaaaggc agcgattcga gagacgaaga ttttatcgaa
     6541 cacatttatc ctgcttcaat gcacaacacc atgttgtttt tcacgcaaaa aggcaaatgc
     6601 tactggctga aagtgtatga aattcccgaa ggcagcaaaa gctcaaaagg tagagccatt
     6661 caaaatcttc tgaatatcga cggagacgat aaagttacag catttatccg cgtaaccggc
     6721 ttaacggatc ccgactacaa cagctcacat tacctggttt tctgcaccaa aaagggaaca
     6781 attaaaaaaa cctcactgga agcctactct catcctcgcc aaaatggagt aacagccatt
     6841 aatattcgcg aagatgatga ggttattcaa gttaggttga ccgatggaaa ttcggaaatt
     6901 ttaatggcta accgcaacgg ccgtgccatt cggttccatg aatcaaccgt gccggaaatg
     6961 ggacgtgttt ctaccggcgt tcgcggcatg aaactcgacg atgaaaaaga tgaagttgtt
     7021 ggcatgattt gtgttaacaa ggaagacaac accaacattc tggtcgtatc tgaacaaggt
     7081 tacggaaaac gaactttact ggatgacgaa aatggagaac caatttaccg cattaccaac
     7141 agaggtggaa aaggcgtaaa aacgatgaat atcaccgaaa aaacaggaaa attggttgcc
     7201 attaagaatg ttaccgatga caacgattta atgattatca acaaatcggg aattgccatt
     7261 cgactgaaag ttgccgatat tccggttatt ggtcgtgcca ctcagggtgt gcgtttgatc
     7321 aacttggaaa aacgcaatga tgagattgct tccgtttgca aagtagattc cgaaccggta
     7381 gaagaaattg ttgacgaaga aacaatagaa accgaattag cggaaaataa cgaaccaaat
     7441 gaaaattaat taaaaaatat ttggaaaaca ataatttgca ttgtaatttt atgccctgaa
     7501 aaaaatattt ttttaaaaaa taaatattta ctgcaagttg attttatttg tcaatcaact
     7561 tgcagtaaat caataggaag agtaaaattt tattgtaaaa aaatttaata ataaaagtat
     7621 gaaaaaaatc attttatctt tctttttggt gctaagtttc accttcactt atgctcaaaa
     7681 gagcaatgtg tcaaaagcga aaaacaaagc attgatggaa aatccagatt ttgcaggagc
     7741 tcgtgaagcc attcaagctg ctttgaacga ttctataaca aaaaatgatc ccaacacttg
     7801 gtatgttgcc ggattaattg gatataaaga aaacgattat tggtatcaga aattgattct
     7861 caatcaaaaa tacgatgctg acgctaaagg acaagccatt ttggaatcgt acaattattt
     7921 caaaaaggct gccgaattgg atcaactccc cgatgcaaaa ggtaaagtaa aaccaaaatt
     7981 tttgaaagat atcaaaacaa aaattaagga atactatacc ttacagccca atttaatcgg
     8041 ctatggtgca tacctttatg aaaagaaaga ctatgaagga gcattaaagg tatttgaaac
     8101 ttatcttgaa attccaactt tgccttacct aaaggatgaa attgtgattg attctactta
     8161 ctatatgata caatattttg cagccctctc cgctactaac gcaggttatc atgataaagc
     8221 catcagcctt tacgaagatt tgaaacataa aaattatgaa accttaaatg tatatgaact
     8281 gttggcagat gaatatagaa ctattaatga taccattaaa tatgttgaaa cgctcaagga
     8341 agggttagaa aagtttcctt ctgaattatg gtttttacaa aatcttatca atcattatat
     8401 ttatagtggt aaaatacagg atgctttggc atatcttaat gatgctatca aacgcgatcc
     8461 gaacattgca caatattatt ttgtaaaggg gaatctggaa gaaaatttgg ggaattacga
     8521 tgaagcaatt tcagctttta acaaagcaat cgaattagat cccaatttag ctgaagcgta
     8581 tgccggcata ggccgattgt actacaacaa agccattaaa atgttggaag ttgccaatga
     8641 tattaaggat gtgaaattgt ttaaccagga aaaagagaaa gcaaataaag tctttaaaga
     8701 atcaattcct tattttcaga aagcaaaaga attgaatccc aacgaaatag aatacaaacg
     8761 tactttgcgt accttgtatt atcgtttgca aatggataag gaatttgaag ctattgatga
     8821 agaaataaga gctatggaaa atcaatgata aaatcaattt ttatacaatt atctatatat
     8881 aggagaaaat tatttagggt tttctccttt ttttttattc atttttaa
//
LOCUS       DAOSIB010000078         8863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_7442_length_8863_cov_11.300749, whole genome shotgun sequence.
ACCESSION   DAOSIB010000078 DAOSIB010000000
VERSION     DAOSIB010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7442_length_8863_cov_11.300749"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PLD44_07185"
     CDS             <1..863
                     /locus_tag="PLD44_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99151.1"
                     /translation="VLAADYFKNPHYLKVAELSAQKMYDDYVSKGITCGGPGDAMQNP
                     DSESGYAMLESFALLYEKTGNEKWLQYAREMAAQFSTWVMSYDYEYSDSCLFGKLNMK
                     TTGAVFANTQNKHGAPGICTYSGLALLRIYRATDNPVYLQLLKEITQAIPQFMSHSTR
                     SVAGMPPGWINERVSTTDWFEGIGEIIPASTFAETAMLLTAVELPSIYVDAEFKTCYT
                     FDHLTSGIVSIKDNKVRLRVTNPTKFDAYFTVLAENKSQKVIRWNENKLLKAKRYFLK
                     AGEQKIIIVD"
     gene            952..2148
                     /locus_tag="PLD44_07190"
     CDS             952..2148
                     /locus_tag="PLD44_07190"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99152.1"
                     /translation="MFIKHKDLKNRHLIHGRPRIVLFGAGKIGRSFIGQLFSRGGYEV
                     VFVDVFQPVIDELNKRHQYNVVIKSDRDEVMIIKNVRGVLATDEKAVISEIANADILA
                     VSIGQQGLPSAIPLIAKGLLQRRKISGDLPLDIIIAENLRNAADFFRAELKKFLPVDY
                     PVDKLVGLVETSIGKMVPIMLKKDIEADILQVFAEPYNNLILDKKAFKNPIPVIEGLS
                     PKENMKAWVDRKLFIHNLGHATTAYLGFKFNPDFVFLYEALAVNEIKSKVRDTMLQAA
                     DILLKKYPDEFTLEALTEHTDDLIFRFQNRALGDTIYRVGCDLKRKLGADDRLAGAIR
                     LALELNMPYDKILEAYIAGCSFKATDEMGNRLQDDIDFSEKFEKYGIDWVLSEIGRLE
                     VEKIRN"
     gene            2256..2924
                     /locus_tag="PLD44_07195"
     CDS             2256..2924
                     /locus_tag="PLD44_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HOF99153.1"
                     /translation="MKRSEINALMRSALDFFAENKFYLPEWATYSPTEWKGKYETHSE
                     IVDNVLGWDLTDFGSGNFEKRGLLLFTIRNGNPAKDNKTYCEKIMIAGVGQETPMHFH
                     RLKMEDIINRGGGVLEIEFYRSNPDGSFSDKPFDVKIDGVLTHLQPGGFVQLKPGQSV
                     TMEAGVYHRFYGVEAPVLVGEVSTVNDDTNDNYFYEEVGRFPQIEEDEAPLFLLSSEY
                     KIYL"
     gene            2978..4174
                     /locus_tag="PLD44_07200"
     CDS             2978..4174
                     /locus_tag="PLD44_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOF99154.1"
                     /translation="MIKIAFTGCGLGDFIYKNIDFNSAAFQKYVSIVPGDGGLVPGQL
                     VFTEELEKFSGKSITKILNDICGLKVYDAFNIGGPALVSAICAAQILFNSEAQIRYFG
                     AFGNDKKAKALLGLLEKLPLNIDNLSLLGDATPYTTVLSDPNYNQGRGERTFINNIGA
                     AGLFDKSFLNTDFWNSNIVVFGGTGIVPQLHSQLTELLALAKNKGVFTVVNTVFDFIS
                     EKKNPDSPWPLGDTLKSLPLIDLLIMDYEEAKRISGQEDFKMITEFYKKNGANAFIIT
                     HGSEPTYIYSSGQKFENVETFLPVCNWISEDFERNPALKGDTTGCGDNFVGGAIASIA
                     MQMNDQNNKLSLIETAVWGTVSGGFACYYTGGTYFEKYWGEKFEKISEMYVRYNNNLK
                     IKNTEN"
     gene            complement(4171..6390)
                     /locus_tag="PLD44_07205"
     CDS             complement(4171..6390)
                     /locus_tag="PLD44_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOF99155.1"
                     /translation="MHKIVLFAFIFCYSLQVNAVIKSSTNVDSLLSLMTLDEKIGQMT
                     QMCFSTITLDGSKSLDLNVDNFKQLIKQQHIGSFLSGSDSYAKWFDFVTAIQKVAVEE
                     TRLGIPLIIGIDHVHGANYINEGTIFPHNLMMACSFDKTLIEKVAKVTAIETAPVGLN
                     WNFSPVLDVARTPYWPRFYETFGEDSRVCAELGSAYIKAYQYVPEIAPYKLSACAKHF
                     IGYSDPKYGYDRSPSEIPMQTLHEVFVPPFRKAFEAGVKTLMVNSGELNGEPVHGSKF
                     LLDTLLRRQLGFNGVIVTDIKDIERIVKMHHTAANEKEATLQVINAGVDMYMACNSYT
                     FIQHVKELLSEGKLSMAQIDASVRRILQLKKELGLFENPYPSKKLLDAVNTTENRKVA
                     LEAAEKSIVLLKNEDILPLSNSTKILVAGIGANSKRMLNGPWSFDWLGAAEAQQPQNM
                     LTLFEACKSTFGTNNVLLSKTERILSPADKKQFLKDTRKADVIVLAVGEQPYSEFMGN
                     INDLTLDADQLELIDAAIATKKPVVLVLIEGRPRVITKQAAGVKAVLFAGYPGIEGAQ
                     AIANILSGKTNPSARLAFSYPLSNGHTIPYNHKPSEFNIYWKPEPAQKWLYPFGHGLS
                     YTQFEYSDLQLSDTIITPNGTLKASVKIKNTGKIRGTETALWYIKDEVGSISRPVKSL
                     KFFEKIDLNSGEEKTIHFEINPQQHLAFPDKAGLYRLEPGRFQLIIDNKSLWFSLNN"
     gene            complement(6698..8698)
                     /locus_tag="PLD44_07210"
     CDS             complement(6698..8698)
                     /locus_tag="PLD44_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF99156.1"
                     /translation="MNILKKALLSAIVFCNVSLNAEVIKTDICIYGATSSGIIAGVSA
                     DRMGKGVVIVEPSQFIGGMSASGLGKTDIGNKMAITGLSRDFYRSIGKYYGKLEMWTF
                     EPHVAETVFSNYLSKTKVSLIKGYEVRALIKSVGKITQITVADVENPTKDPITIQAKM
                     FIDASYEGDLMAKAGVSYTIGRESNATYNETYNGFQLSEKHQFPDSISPYINPKNTNS
                     GYVYGVSNNIPVATGTGDKLVQAYNYRLCLTKNEDNKIPIQKPENYDPQKYELLRRVI
                     AQREKKKWKHLLVSYFNIGHLPNGKTDINNNGPFSTDFIGENYNYPEADYKTRKQIEQ
                     KHIDYIKGLLYFMAYDPAVPDTLRQQMQAWGYCKDEFQKTGGFPHQIYVREARRMIGE
                     YVMTEHNCLGKEKIKDGVGMAAYGMDSHNCQRIAINGFVKNEGDVQIGGFPPYPISYR
                     SITPKRNECTNLLVPVCLSASHIAYGSIRMEPVFMVLGQSAAIAASLAIDQNIAVQKV
                     NVKTIQSILAKNPYLDGRKPEVLVDNSDIKQVKLKGKWELPAKPIASNKMDYLMVVAD
                     GKSATEVLFTPKMVDFGMYDVYLYCPEKPINTPEWAPSVDVEIFTGQDTNKITVDQKA
                     NSHHWVKLGRYELNYKKNISVKVIANKPGVVIADAVLFVPVK"
BASE COUNT         2660 a         1691 c         1785 g         2727 t
ORIGIN      
        1 tggttttggc tgccgattat tttaaaaatc cgcactatct gaaagtcgct gaactttcgg
       61 cgcaaaaaat gtacgacgat tatgtgtcaa aaggcataac ctgcggcgga cctggcgatg
      121 ctatgcaaaa tccagatagc gaatcaggct atgccatgct cgaatccttt gcattgctct
      181 acgaaaaaac cggaaatgaa aaatggttgc agtatgcccg cgaaatggct gcgcaattct
      241 caacctgggt tatgagttac gattatgaat attccgacag ttgtcttttt ggaaaactga
      301 atatgaaaac gaccggagct gtatttgcaa atacccaaaa caaacacggt gcacctggaa
      361 tttgtaccta ttcaggtttg gcacttttac gtatttatcg ggctacagac aatccggttt
      421 atctgcagtt gttgaaggaa ataacacagg caattcctca atttatgtcg cactccacac
      481 gttcggttgc cggcatgcct ccgggttgga taaatgaacg tgtgagtacc accgattggt
      541 tcgagggtat tggtgagata ataccggcct caacctttgc cgaaacggct atgttactta
      601 ctgccgttga attgccttcg atttatgtcg atgctgagtt taaaacttgc tatacgttcg
      661 atcatttaac ttccggaatt gtgtcgataa aagataataa agtacgattg agagttacca
      721 atccaacgaa atttgatgcc tattttacgg ttttggctga gaataaatct caaaaagtaa
      781 tccgttggaa tgaaaataaa ttactgaaag caaaaagata ttttttgaaa gctggagaac
      841 aaaaaataat aatagttgat tgaagaacga ttgtgtaata gggtaaatgc ttgtttctgc
      901 attttataaa acaagggaaa gctacttcat tattcaaagt ctcaaaaatt catgttcatt
      961 aagcacaaag atctaaaaaa cagacactta attcacggac gaccacgtat agtcctattt
     1021 ggcgcgggaa aaataggtcg ctcttttatt ggtcagttat ttagcagagg gggctacgaa
     1081 gtagtttttg tagatgtttt ccaacccgta attgacgaat taaacaaaag acaccaatac
     1141 aatgtagtca ttaaatcaga tcgggatgag gtgatgatta tcaaaaatgt acgtggagtt
     1201 ttggcaaccg acgaaaaggc tgtgatttca gaaattgcca atgctgatat tttagctgtt
     1261 tcaatcggtc agcagggatt gccttcggct attccgctca ttgctaaagg attattacaa
     1321 cggagaaaga tttcaggtga tttgcctttg gacatcatta ttgctgaaaa tttgcggaat
     1381 gcagcagatt ttttcagagc agaactcaaa aagtttttac ctgtagatta tccggtggat
     1441 aaattggtgg gattggtaga gacaagcata ggtaaaatgg ttcctattat gctcaaaaag
     1501 gacatagaag ccgatatttt gcaggttttt gctgaacctt acaataatct gattttagat
     1561 aaaaaggcat ttaaaaatcc cattccggtt attgaaggtt tatcgcccaa agaaaatatg
     1621 aaagcctggg tggatcgtaa gttgttcatt cacaatttgg ggcatgcaac tacagcttac
     1681 ctcggattta agtttaaccc cgatttcgta tttttgtacg aagcattggc agtgaacgaa
     1741 attaaaagta aagtgcgcga taccatgttg caggcagccg atattttact gaaaaaatat
     1801 cctgatgaat ttacattgga agcactcact gaacataccg acgatttaat atttcgcttt
     1861 caaaatcgag cgttaggcga caccatttat cgtgtgggct gcgatttaaa aagaaaatta
     1921 ggtgctgatg acaggctggc aggtgccatt cgattagcgt tggaactaaa tatgccttac
     1981 gacaaaatac tcgaagccta tattgccgga tgtagcttta aagccaccga cgaaatggga
     2041 aacaggttac aggatgatat tgatttttcg gagaaattcg aaaaatatgg catcgattgg
     2101 gtattaagtg aaattggaag attagaagtt gagaaaatta gaaattaaaa aaatatcgct
     2161 ctgtgcaact tttttggaaa aagtaaaatc ctgagaatta aaaatcagaa atggtaagga
     2221 gtaaattgta aatgaacaac cagtaaatca atattatgaa gcgaagtgaa atcaatgcat
     2281 taatgcgctc ggcattagac ttttttgccg aaaataaatt ttatttgcct gaatgggcta
     2341 catacagtcc aactgaatgg aaaggtaaat acgaaacaca ttccgagatc gttgacaatg
     2401 ttctcggttg ggatttaacc gattttggca gtggtaattt cgaaaaacgc ggtttgttac
     2461 tttttaccat tcgaaatgga aatcctgcaa aggacaataa aacctattgc gaaaaaataa
     2521 tgattgccgg agtaggacag gagacgccta tgcactttca tcgcctgaaa atggaagata
     2581 ttatcaatcg tggtggtgga gttctcgaaa tagagtttta ccgctcaaat cccgatggaa
     2641 gtttttcgga taaacctttt gatgtaaaaa ttgatggtgt acttacgcat ttgcagccgg
     2701 gtggattcgt tcaacttaaa cccggtcaaa gtgtaacaat ggaagctggc gtttatcatc
     2761 gtttttacgg tgttgaagca cctgttttgg tgggcgaagt aagtacggta aacgatgata
     2821 ccaacgataa ttatttttac gaagaagttg gtcgtttccc gcaaattgaa gaggatgaag
     2881 ctcctctctt tttgttgtca agcgagtaca aaatttatct ataaggttta atgaatatca
     2941 atcaattcct cctgaagggg ttgagggggc ttattatatg atcaaaatag catttacagg
     3001 ttgcggtctg ggcgacttta tttataaaaa cattgatttt aattccgcag catttcaaaa
     3061 atatgtatca atcgttcccg gagatggcgg acttgttccc gggcaactcg tttttaccga
     3121 agagctggag aagtttagcg gaaaatccat tactaagatt ttgaatgata tctgcggatt
     3181 aaaagtttac gatgcattta atatcggcgg accggctttg gtgagcgcca tttgtgctgc
     3241 acagatttta tttaattccg aggctcaaat aagatatttc ggagcatttg gtaacgataa
     3301 gaaagcgaaa gcgctacttg gattgttaga gaaattaccg ttgaatattg ataatttatc
     3361 gttgttgggt gatgcaacgc cttatacgac agtgctttcc gatccgaatt acaatcaggg
     3421 aagaggcgaa cgtactttta tcaataatat cggagctgca ggtttatttg ataaaagttt
     3481 tttaaatact gatttttgga attcaaacat tgttgtattc ggaggaacgg gcatagttcc
     3541 ccaattacat tcacaactta ctgaattatt agctttggct aagaataaag gagtatttac
     3601 ggttgtcaat actgttttcg actttatcag cgaaaagaaa aatccggaca gtccctggcc
     3661 tttgggcgat acgctgaaaa gtttgccatt aattgattta ttaataatgg attatgaaga
     3721 ggcaaagcga ataagtggac aggaagattt taaaatgata actgaatttt acaaaaagaa
     3781 tggagcaaat gcctttatca ttacacacgg aagtgagcca acttacattt attcttcagg
     3841 ccaaaagttc gaaaatgtcg aaacattcct gccggtttgc aattggatat cggaagattt
     3901 cgagagaaac cctgctttga aaggagatac aaccggctgt ggcgacaatt ttgtgggcgg
     3961 tgccattgca tcaatagcta tgcaaatgaa tgatcagaac aataaacttt cattaatcga
     4021 aactgctgtt tggggtactg tttctggagg ttttgcctgt tattataccg gaggaaccta
     4081 tttcgaaaaa tattggggcg aaaagtttga aaaaattagc gaaatgtatg taagatacaa
     4141 taacaatcta aaaataaaaa acacagaaaa ttagttgttc aacgaaaacc acaaactctt
     4201 attgtcaatt atcaactgaa accttccggg ttcgagacga taaagccctg ctttatcagg
     4261 aaatgcaaga tgttgttgtg gattgatttc gaaatgtatt gttttttctt cacctgaatt
     4321 caggtcaatt ttctcaaaga actttagact ttttaccgga cgggaaattg agccaacttc
     4381 gtctttaata taccacaagg ctgtttctgt tccacgaatt ttacctgtat ttttaatttt
     4441 tacggaggct ttcaaggttc cattaggagt aataattgta tccgaaagtt gcaaatcgga
     4501 atattcaaac tgtgtatagc tcaatccatg accgaaagga taaagccatt tctgagcagg
     4561 ttccggtttc cagtaaatgt taaattccga cggtttgtgg ttgtacggaa tggtatgtcc
     4621 gttactcagt gggtacgaaa atgccaatcg ggcagacgga ttggttttgc ccgaaaggat
     4681 attggcaatg gcctgagcgc cttcaatgcc gggatatccg gcaaaaagga cggcttttac
     4741 tccggctgcc tgcttggtta ttacacgcgg acggccttcg atcaaaacca acacaactgg
     4801 ttttttagtg gcaatggcag catcaatcag ttcaagttgg tcggcatcga gcgtcagatc
     4861 gttgatattt cccataaact cgctgtaagg ctgttcgcct acagccaaca caatcacatc
     4921 agcctttcgg gtatctttca gaaattgttt tttatcagca ggagataaaa tgcgttcagt
     4981 ttttgataac aggacattat tagttccaaa agtacttttg caggcttcga ataaagtgag
     5041 catgttctgt ggttgctgtg cttctgctgc tcccagccaa tcgaacgacc agggaccatt
     5101 gagcatccgc tttgaattag ctccgattcc tgctactaat attttcgttg aattgcttag
     5161 tggcagaata tcttcatttt tcaacaatac gatcgatttt tcggcagcct cgagcgctac
     5221 ttttcggttt tcagtggtat ttacggcatc caataacttt ttggaaggat acggattttc
     5281 gaaaagtccc aactcttttt tcaattgcaa aatacgacgt accgaagcat caatctgcgc
     5341 catcgataat tttccttcgg acaataactc cttgacatgc tgaataaacg tgtatgaatt
     5401 acaagccatg tacatatcaa cacctgcatt gattacttga agtgttgcct ctttttcatt
     5461 cgcagctgtg tgatgcattt ttacaatgcg ctcaatatct ttaatgtcgg taactattac
     5521 accattaaag ccaagctgac ggcgaagcaa agtatcgagt aaaaacttcg agccatgcac
     5581 cggttctccg ttcagttcgc cgctattgac catcagggtt ttcactcctg cttcaaaagc
     5641 ttttcggaat ggaggcacaa atacttcatg gagcgtttgc ataggaattt cggacggtga
     5701 gcggtcgtaa ccgtattttg ggtcggaata gccaataaag tgcttggcac aggccgaaag
     5761 tttgtaaggt gcaatttccg gaacatattg ataagctttt atataagcgc tccccaactc
     5821 tgcacaaacc cgcgagtctt cgccaaaagt ttcgtaaaag cgcggccagt aaggtgttcg
     5881 ggcaacatcg agcactggag aaaaattcca gttcaaacca acgggagcag tctctatcgc
     5941 agttaccttt gctacttttt caatcagggt tttatcaaaa ctacaagcca tcatcaggtt
     6001 gtgtggaaaa atggtgcctt cgttgatata attggcgccg tgcacatggt caatacctat
     6061 tattaatgga ataccgagcc gcgtttcctc cacagccact ttttgaattg ccgtaacaaa
     6121 gtcgaaccac ttagcataac tgtcactacc gctcaaaaac gaaccaatat gctgttgttt
     6181 aatcaactgt ttgaaattat ccacattcag gtcgagcgat ttactgccat ccagggtgat
     6241 ggtactgaag cacatttgcg tcatttgccc gattttttcg tcgagcgtca ttaacgaaag
     6301 taaagagtcg acattggttg atgatttaat tactgcattt acttgtaatg agtaacaaaa
     6361 aatgaaagca aataaaacta tcttatgcat attataatta aatttgaact tagtacatga
     6421 caaaaaatgt agaaaaattt atattcttta ttttcagata tttaataatg tggctttgcc
     6481 ccaaacccat tacaagaaaa attgacgttc gttggctaaa atcttctaaa ctcgctcctc
     6541 cgtcgctcaa acagagaaga ttttttaacg cgaacttcac tgatttttcg gctcaccgga
     6601 caaagtcaac cccaagagct tggcttattt gttctgtttt acagatatat aacataaatg
     6661 tctcaatttt tgtcatgtac taaaaagaat tttacattta ttttacagga acaaaaagca
     6721 ctgcatcggc tatcacaaca cccggcttgt ttgcaataac ttttaccgaa atgttttttt
     6781 tataatttaa ttcataacgc cccaatttta cccagtgatg gctatttgct ttttgatcca
     6841 cggttatttt atttgtatct tgtccagtaa aaatttcgac atcaacagac ggagcccatt
     6901 caggtgtatt aatgggtttt tctggacaat agagatatac atcatacatg ccaaaatcga
     6961 ccatttttgg agtaaatagc acttctgtag cactttttcc atcggccaca accatcagat
     7021 aatccatttt atttgatgca ataggttttg caggtaattc ccatttaccc tttagtttta
     7081 cttgcttaat gtctgaatta tcgaccaaca cttcaggttt tcgtccgtct aagtatggat
     7141 tttttgccaa gattgattgt atagttttta cattcacttt ttgcacagca atgttttgat
     7201 caattgccaa agaagcagct attgcagccg attgtcccaa caccataaat acaggttcca
     7261 tgcgaatgga gccataggcg atatgcgaag ccgataagca aacaggcact aacaaattgg
     7321 tacattcgtt tcgtttgggt gtaatagaac gatatgaaat tgggtaggga gggaaaccac
     7381 ctatttgtac atcgccctcg tttttcacaa aaccattgat cgcaatccgc tgacagttat
     7441 gcgaatccat tccgtaggct gccattccga caccatcttt tattttttct ttgcccagac
     7501 aattgtgttc ggtcattaca tattcaccaa tcatacgccg tgcttcgcga acatagattt
     7561 ggtgcgggaa acctccggtt ttctgaaatt catctttgca gtagccccat gcctgcatct
     7621 gctgacgtaa cgtatccgga actgcagggt cgtaagccat aaaataaagt aaccctttga
     7681 tatagtcaat gtgtttttgt tcaatttgtt ttctggtttt atagtctgct tccgggtaat
     7741 tatagttttc gccaataaaa tcggtcgaaa acgggccatt gttatttatg tctgtcttcc
     7801 cgtttggcaa atgcccaata ttgaaataag agactaatag gtgcttccac ttcttttttt
     7861 cacgttgggc tataactcta cgaagcaatt catatttttg cggatcataa ttttccggtt
     7921 tctggatagg aattttatta tcttcatttt ttgttaagca caagcggtaa ttatacgctt
     7981 gcactaactt atcgccggtt cctgtggcga cagggatatt gttactcacc ccgtagacat
     8041 aaccactgtt cgtatttttt ggattaatgt atggacttat actatcggga aactgatgct
     8101 tttcacttaa ctgaaagcca ttatacgttt cgttataagt agcattgctt tcccttccta
     8161 tcgtgtatga aactccagct tttgccatca aatctccttc gtaactggca tcaataaaca
     8221 ttttggcctg tatcgtaatt gggtctttgg tcggattttc aacatcggca accgtaatct
     8281 gagtgatttt acccacagac tttataaggg ctctcacttc atatcctttt atcagactta
     8341 cctttgtttt tgaaaggtaa ttactaaaaa cagtttcagc cacatgtggt tcaaaagtcc
     8401 acatttccag cttgccgtaa tattttccga ttgagcggta aaaatcgcgc gacaatcccg
     8461 ttattgccat tttattgcca atatccgtct tgcctaatcc gctagccgac ataccaccga
     8521 taaactgaga tggttccaca ataacgacac ctttacccat acgatcggct gaaacgccag
     8581 caattatccc cgacgaagta gctccgtaaa tgcaaatatc ggttttgatt acttcggcat
     8641 ttaaacttac attacaaaac acaatagcac ttaacaatgc tttttttaga atattcataa
     8701 ttttatgttt tcaaaaattt aataccaaac acttacacca cccgggttca gagcgcttgt
     8761 tccaatgtaa aaatttgagc tatttattgt tggtaaaaca actgtttggg aactgtaatt
     8821 cacagccacc caatagccgt cgcgccattc tacaaaaacg taa
//
LOCUS       DAOSIB010000079         8592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_7689_length_8592_cov_11.769123, whole genome shotgun sequence.
ACCESSION   DAOSIB010000079 DAOSIB010000000
VERSION     DAOSIB010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7689_length_8592_cov_11.769123"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PLD44_07215"
     CDS             <1..684
                     /locus_tag="PLD44_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein BatD"
                     /protein_id="HOF99157.1"
                     /translation="NFEIPPVEFSYFDLRDKTYKMLRTPSYSLKVLKGSGNQNATVVS
                     NYNPKEDIRQINKDIRYIYTGNIRLIKPSTPVFGTTLAWLLFIIPLVVVVGLFFFFRK
                     KVKENADIALVRNKKANKMAQKRLKLAQKLLKEGNKDAFYDEILKATWNYLSNKLSIP
                     VASLTRETVSEELKLRGVADELTKKLMKILNDCEFARYAPKSGQQEMGNVYEETIEVI
                     SELEEAIKK"
     gene            793..1551
                     /locus_tag="PLD44_07220"
     CDS             793..1551
                     /locus_tag="PLD44_07220"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOF99158.1"
                     /translation="MKRLISNIILIITLLASLPLSAQSDAIKYANDLYAKGNFHAAAE
                     QYEKILKNEGIAPELYYNLGNAYYKMNEIGLAILNYERALKLSPRFEDARFNLQLAQQ
                     KVLDNVNYNQQFFIQRWITNLMHMFTSNQWFTAGYLFFLAALIFAFLFAFGSSVRQRK
                     NSFYIGIVLLLFAVTSLIFSGIRKNEETNSLQAIIMTGVVTVKSSPDESGTDLFQLHE
                     GTKVTIKSTLGNWAEIKIGNGNVGWVESKSIEKI"
     gene            1591..2274
                     /locus_tag="PLD44_07225"
     CDS             1591..2274
                     /locus_tag="PLD44_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOF99159.1"
                     /translation="MNLIETLRIWDTNLFLALNGIHSPFFDGFMMAFSNKLVWIPFYI
                     SVLYVMIKQWKSEAFWIVLSFAICILLADQISSGIIKNAVQRLRPSREETISGLVHLV
                     DGYRGGKYGFVSSHAANSLGFAVLSAYFFRKKSYTLIILLWSLITGYSRIYLGVHYPL
                     DVMGGYLVGFFVAIFCYWLLKKLKPNIFYVSSRFRSKRFADSTLPLLVLTFTVVGIFG
                     WSLIRILAF"
     gene            2362..2434
                     /locus_tag="PLD44_07230"
     tRNA            2362..2434
                     /locus_tag="PLD44_07230"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2395..2397,aa:Thr,seq:cgt)
     gene            2456..2812
                     /locus_tag="PLD44_07235"
     CDS             2456..2812
                     /locus_tag="PLD44_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99160.1"
                     /translation="MMFDNILFASFVPQILMILGYIMCLSAATSSSHVSGISEITPIT
                     QHQVFFSTENKIETENTFQFEDYFFVSAIIPSKESIKPIYYFPTIIPLVKCNACISNG
                     FLAELFCRPPPTLSFC"
     gene            3080..5338
                     /locus_tag="PLD44_07240"
     CDS             3080..5338
                     /locus_tag="PLD44_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOF99161.1"
                     /translation="MKKILILLSLVPTFIFAVDLEPKDSLKHVYLEEIQIISHKETLP
                     AQPAASYSVVSSNLINQAQVNSVRDLSILVPNFFIPDYGSSMSTAPYIRGVGARSTGQ
                     SIALYMDNVPYFEKSTFDFDFYDIKQAEVLRGSQSTLYGRNALGGVVNIYTLSPFEYQ
                     GTKLSVTGGNYGLLQAQASHYAKLSSKTGLSLSGYYHHRNGFFTNTFTGKDADWKNSA
                     GGRAKFDWHITPSLRAQYIINYDYVNQNAFPYGLYNATTKSTAEPNFNDKSSYLRNML
                     TNSVLIEYRNDKIQLTSTTSYQYFSDKMFMDQDFTPLSYFSLKQQQKQHAVNEEIVLK
                     SRTSGNYQWSFGATGFYQKLNTDAPVAFKQDALTNILQPVLDRAHTAGAPQMTITSTV
                     MDIPGTYDTKTAEGALYHQSTFNHLLTDGLSATIGVRADFEKVNLDYFTNTRLDLLMK
                     MGNRNIPTSLADTLRGAESVKFSEFLPKVSVKYAWNSQQYIYANVSKGYKAGGYNIQM
                     FADLIQNDLMNSRNPSASQPDVRKAILFQPEYSWNYEAGCQSTFLDNKLKMQLSLFYM
                     QITDMQLTRFVPSGNGRMITNAGKVDSKGMEFSLQANLGKGFAAGLNYGYAHAAFTNY
                     TDSVKSNGTVNEVDYKGKFVPYAPQNTLNIGGEYLRIFKNSFIDQFMVSMQYNAAGKI
                     YWDVDNSIYQDFYGLINAKVGVGKGNFRVELWAKNLFDTQYNAFYFESFENKFFQKGN
                     PAQFGLTLKTEF"
     gene            5493..6299
                     /gene="mazG"
                     /locus_tag="PLD44_07245"
     CDS             5493..6299
                     /gene="mazG"
                     /locus_tag="PLD44_07245"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOF99162.1"
                     /translation="MEKTTHTMEEKLQEFKRLLEIMERLRAECPWDREQTFESMRVQT
                     IEETYELADAIMKDDKKAIMTEVGDLLLHVVFYAQMGSETGDFDIFDVCKNLNEKLIF
                     RHPHVFSNTKADNSQTVVQNWEDLKLKEKNGNRTVLAGVPETLPALIKAHRIQDKARN
                     VGFDWEKREQVWDKVQEEFQELKDEIEAMDKNKMEEEFGDLLFSLINAARLYDINPEN
                     ALERTNRKFIQRFNYLESQTISQGKDLRQMSLDEMDVFWEEAKKKEKQEG"
     gene            complement(6399..8279)
                     /locus_tag="PLD44_07250"
     CDS             complement(6399..8279)
                     /locus_tag="PLD44_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOF99163.1"
                     /translation="MFIAIFILISISTFSLKAQLFRDSILKNEEKTIYMNEVIVTGTR
                     SEADVRNLPMSLSVITDKQILDRQSQSVIPLLNELVPGLFITGRGIMGYGISTGAAGT
                     MKMRGIGGNPTTGVLILIDGEPQFMGLMGHPIADLYQSVIADRIEVVRGPASALYGSN
                     AMGGVINIITSKFYKDTVQQNFHFGYGSYSTLESSASNHFHKNRITGKFAISYNRTDG
                     HRENMDFDQISGYAKLGYRLDRYWSVSTNANISHFNASNPGTVDQPIVDNDSHVKRGW
                     INASVENRYRYTSGALSFFYNWGNHIINDGYNMGGQPLDYLFHSNDKISGITFYQSAI
                     LMPNNKITFGFDYQKFGGKAWNKYNDGNKKSIIDTTLYEIAGYADMRQDFGLLATVDI
                     GIRLDNHSKTGSQWIPQVGLSFHLPKNIEAKTIVGKGFRNPTIRELFMFTPQNPNLLP
                     EEVMNYEFSVSQNMVEKNLFYDVAVFYLEGKNMIQTVISNGRPINVNTGKIKNFGFES
                     VITYQINSIWSANVNYSWLDMKYPVTGAPKQKVFAGFDYSKKKLKFSSGIQYVHRLYS
                     SLNPVKKENFTLLNARTTYQLGKFTQLFLTGENLLNCSYEINAGYPMPKATFLIGCNV
                     HF"
     gene            8480..8592
                     /locus_tag="PLD44_07255"
                     /pseudo
     CDS             8480..8592
                     /locus_tag="PLD44_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013265017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE transposase"
BASE COUNT         2699 a         1617 c         1613 g         2663 t
ORIGIN      
        1 aattttgaaa ttccaccggt agaattttcc tatttcgatc tccgtgacaa aacttataaa
       61 atgttgcgca ctccgagtta cagcctgaag gtattgaaag gttcgggcaa tcaaaacgcc
      121 actgttgtaa gcaactataa tccgaaagaa gatatccggc aaatcaacaa agacattcgc
      181 tacatttaca caggcaacat ccgtttgata aaaccatcaa ctcctgtttt cggcactacc
      241 ctggcatggc tgctgttcat tattccattg gttgtggttg ttggtttatt cttcttcttc
      301 cgcaaaaaag tcaaggaaaa cgcagatatt gctttggtga gaaataaaaa agccaacaaa
      361 atggctcaga aaaggttgaa actggctcaa aaattactta aagaaggcaa caaggatgct
      421 ttttatgatg aaattttgaa agccacttgg aattatttga gcaacaaact ctccatacct
      481 gttgcctcac ttaccagaga aacggtttcg gaggaattga aactaagagg agtagccgat
      541 gaattgacaa agaagttgat gaaaattttg aacgattgtg agtttgcacg atatgcacca
      601 aaatccggac aacaggaaat gggtaatgtt tacgaagaaa ctattgaagt aatcagcgaa
      661 ttggaagaag ccataaaaaa gtaaacaatt cgcagatagc gaagataagc ttgtttaaaa
      721 caccatttta tcttaacaaa taaaatatcg gctgtgctgt tacaaaccga ataaaaaatt
      781 taaaaataaa ctatgaaacg tctcatatcc aatatcatac ttataataac tctcttggct
      841 tcgctgcccc tctcagcgca atcggatgcc attaaatatg ccaacgacct ctacgccaaa
      901 ggaaattttc atgctgctgc agaacagtac gaaaaaatac taaaaaacga aggcatagcg
      961 cccgagctct actataattt gggaaatgcc tattataaaa tgaatgaaat cggattggcc
     1021 atcctgaatt acgaaagggc tttgaaactt tcaccacgct ttgaggatgc ccgattcaat
     1081 ttgcaactgg cacaacaaaa agtattggac aatgtcaatt acaatcaaca attttttatt
     1141 caacgctgga ttaccaattt gatgcacatg ttcacttcca accaatggtt tacggcgggt
     1201 tacctgttct tcttggctgc cttgattttt gcttttcttt ttgcatttgg ctcatcggta
     1261 cgacaacgaa aaaattcttt ctatatcgga attgttctgc ttctctttgc tgttaccagc
     1321 cttatttttt ccggcattcg caaaaacgaa gaaaccaatt ctcttcaagc tattattatg
     1381 acgggagtgg ttacggtgaa aagctcaccc gacgaaagtg gaacagattt gtttcagctg
     1441 cacgaaggaa caaaggttac aataaaaagt acattgggta attgggcaga aatcaaaatc
     1501 ggtaatggaa atgtgggctg ggtagaaagc aaaagcatag aaaaaatata aaaaggttac
     1561 tggttttcac ggttgcaaat acttcggaaa atgaacctga tagaaacatt aagaatttgg
     1621 gataccaact tgttcctggc cttgaatggc atacacagcc cattttttga cgggttcatg
     1681 atggctttca gcaacaaact ggtatggatt ccgttttata tttccgtttt gtacgtgatg
     1741 attaaacagt ggaaatcgga agctttctgg attgttcttt cttttgccat ttgtatttta
     1801 ctcgccgatc aaatttcatc gggaattatt aaaaatgccg ttcaacgcct gcgtccttcg
     1861 cgcgaagaaa ctattagtgg gctggttcat ttggtggatg gttatcgtgg tggaaaatat
     1921 ggattcgttt cttcgcatgc tgccaattca cttggttttg ccgttctttc ggcttacttt
     1981 ttccgtaaaa aaagctacac gctgattatt ttactctggt cgttgattac gggctattca
     2041 cgaatatatt tgggtgtaca ttatcctctt gatgtcatgg gagggtattt ggtcggattt
     2101 tttgtagcta ttttttgcta ttggttacta aaaaaactga aaccgaatat tttttacgtt
     2161 tcttcccgtt ttcgaagcaa gcgttttgct gattcaacct taccgctatt ggtgcttacc
     2221 ttcacggttg tgggaatttt tggttggagt ctgattcgga tattggcttt ttaaagaatt
     2281 gcttgttcta aaaattttga cttcaacacc tgcaaatatc tcgatttttt tgattaattt
     2341 tgcagccgtt aataaaatag tgccttttta gctcagctgg tagagcgatt cattcgtaat
     2401 gaataggtct ggggttcgag tccccaaaaa ggctctttat aacgatttcc aaaagatgat
     2461 gtttgacaat attctttttg cttcttttgt tccacaaata ctgatgatat tgggttatat
     2521 catgtgtttg tcggcagcta cttcatcttc tcatgtttca ggcattagtg aaattactcc
     2581 cataacacaa catcaagtat ttttttcgac tgaaaacaag attgaaaccg aaaatacttt
     2641 tcaatttgaa gattattttt ttgtttcagc catcattcca tccaaagaat caataaagcc
     2701 aatttattac tttcccacaa taattcctct tgtaaaatgt aatgcctgca tttcaaatgg
     2761 tttcttggca gaactttttt gtcgtccacc tcctactctc tccttttgtt aaatcaataa
     2821 atatttcctg tgtttcggca attacactta taatttttgc cagaaaccct tcattaatgg
     2881 ctgtttgttg cagttaaaaa gacgaaagaa aatcgaaaaa aattggacgt ttttattgaa
     2941 ttattgagct ttaattcaat ctgaaaaaac tcaataaacg aaaatcggtt tttttaacaa
     3001 tcttgcagca tattgcatac aaaaaatgct gaataaatat tcactattgt attcgtttat
     3061 ctatattaaa atcaaaaaaa tgaaaaaaat attgatatta ctttctttag ttcctacctt
     3121 tatttttgcg gttgacttgg aaccgaaaga ttcattgaaa catgtttatc tcgaagaaat
     3181 tcagattatt tcacacaaag aaaccttacc tgcacaaccg gctgcttctt attcggttgt
     3241 gtcatccaat ctcatcaatc aggcacaagt caattccgtt cgcgacttga gcatactcgt
     3301 tcccaatttt tttattcccg attacggttc ttccatgtca acggctccct acattcgtgg
     3361 tgttggagca cgcagcaccg gacaatcaat agccttgtat atggacaatg ttccatattt
     3421 tgaaaaatca acgttcgatt tcgatttcta cgacataaag caggctgaag ttttgcgtgg
     3481 ttcacaaagt actttgtacg gacgcaatgc tttggggggc gtggtaaata tttatacact
     3541 ttctcccttt gaatatcagg gaacaaaatt atcggttaca ggtggtaatt acggtttatt
     3601 gcaagcgcaa gcttcgcatt atgccaaact gagcagcaaa acaggattgt cgctaagcgg
     3661 ttattatcat catcgcaacg gattctttac caatactttt accgggaaag atgccgattg
     3721 gaaaaattct gccggaggta gagctaaatt cgactggcat attacgcctt cgctgcgtgc
     3781 tcaatacatt atcaattatg attatgtgaa tcaaaatgca ttcccttacg gactgtataa
     3841 tgcaactacc aagtcgactg cagaaccgaa ttttaacgat aaaagttcct atttacgcaa
     3901 catgcttacc aatagtgtac tgattgaata tcggaacgac aaaatacaac tgacttctac
     3961 tacttcttac cagtattttt cggataaaat gtttatggat caggatttta cgcctctttc
     4021 ctacttttct ctcaaacaac agcaaaaaca acatgcagta aacgaagaaa ttgtattgaa
     4081 atcgagaacc tccggcaatt atcagtggag tttcggagct acaggctttt atcaaaaatt
     4141 aaataccgat gcgcccgtag ctttcaagca ggatgcgttg accaatatcc tgcaaccggt
     4201 actggacagg gcacatactg ccggagcgcc acaaatgacc attaccagta ctgtaatgga
     4261 cattcccgga acttatgaca caaaaaccgc tgaaggggct ttgtatcacc aatcgacctt
     4321 caatcatctt ttaaccgacg gactttcagc gacaataggt gttcgtgccg attttgaaaa
     4381 agtaaattta gactatttta ccaatacgag actggattta ttaatgaaaa tgggaaatag
     4441 aaacattcca acttcattgg ccgatacact tcgaggagca gaatcagtga aattttccga
     4501 atttcttcca aaagtcagtg tgaaatacgc atggaacagc cagcagtaca tttatgccaa
     4561 cgtttcgaaa ggttacaaag caggaggtta caacatacag atgtttgccg acttgataca
     4621 gaatgattta atgaattcaa gaaatccgtc tgcttctcaa cccgatgttc gcaaagccat
     4681 tttgtttcaa ccggaataca gttggaatta cgaagcaggc tgccaatcga catttttgga
     4741 caacaaactg aaaatgcaat tgtcgttgtt ttacatgcaa atcaccgata tgcaactaac
     4801 ccgatttgtt cccagcggca acggacgaat gataaccaac gcaggcaagg tggacagcaa
     4861 aggaatggag ttctcattgc aggcaaattt gggaaaaggt tttgccgccg gtcttaacta
     4921 tggttatgca catgcagcct ttaccaatta taccgattcg gtaaaaagta acggaactgt
     4981 aaacgaagtg gattacaaag gaaaatttgt tccttatgct cctcaaaaca ccttgaatat
     5041 cggaggcgaa tatcttcgca tattcaaaaa ctcgtttatt gatcaattca tggtttcaat
     5101 gcaatacaac gcagccggga aaatctattg ggatgtggac aattccattt atcaggactt
     5161 ttatggttta atcaatgcaa aagttggtgt tggaaaggga aatttcaggg tcgaactttg
     5221 ggcaaaaaat ctgtttgaca cacaatacaa tgctttttat tttgaatctt ttgagaacaa
     5281 gttttttcaa aaaggaaatc ctgcacaatt cggtttgact ttaaaaaccg aattctgaaa
     5341 aaacttttaa aaaataactt ttgcttcagt atcattgatc cattacgaga aaaacttatt
     5401 ctcgtaatgg attttatatt ttcgtaaaaa tctcttcatc aaaaaaaata agtaatttcg
     5461 cataaaattt atacatactt caccctgaaa ttatggaaaa aacaacacat acaatggagg
     5521 aaaaacttca ggaatttaaa cgtttgctgg aaataatgga gcggttgaga gccgaatgcc
     5581 cttgggatag agaacaaact ttcgagagca tgcgcgttca aactattgaa gaaacttatg
     5641 aattggccga tgcaataatg aaagacgaca aaaaagccat catgacggaa gtaggcgact
     5701 tgttgcttca tgtggttttt tatgcacaaa tgggaagcga aaccggcgac ttcgacattt
     5761 ttgatgtttg taagaatctg aacgaaaaac tgatttttcg ccatcctcac gtattttcca
     5821 ataccaaagc cgacaacagc caaacggtcg ttcaaaattg ggaagatttg aaattaaagg
     5881 agaaaaacgg caaccgaact gttcttgccg gcgttcccga aactttaccg gcattgataa
     5941 aagctcatcg gattcaggac aaagcgcgaa atgttggatt tgactgggaa aaacgcgaac
     6001 aagtatggga taaggttcag gaagaatttc aggaactaaa agacgaaata gaagcgatgg
     6061 ataaaaataa gatggaagaa gaatttggcg acttgctttt cagcctgatc aatgcagccc
     6121 gcctttatga catcaatccc gaaaatgcac tcgaacgaac caaccgtaaa tttattcaac
     6181 gattcaatta cctcgaatct caaaccattt cgcaaggaaa agacctgaga caaatgtcgc
     6241 tggacgaaat ggatgttttc tgggaggaag ccaaaaagaa agagaaacaa gaaggttaaa
     6301 aatcaatttt atttttttct ttccctgcag caaagcctta ataaagtatt agccaacaat
     6361 tatttatcat ttgaataatt gaaagaaatc gaagtttttt aaaaatggac attgcaacct
     6421 attaaaaatg tggcttttgg catgggataa ccggcattta tttcataaga gcagtttaaa
     6481 agattttcac ccgttaaaaa tagttgggtg aatttaccca attggtacgt cgttctggca
     6541 ttcaacaagg taaaattttc ctttttcaca ggattgagag aagaataaag tctatgaaca
     6601 tattgaattc cggaagaaaa ttttagcttt tttttcgagt agtcgaatcc tgcaaaaact
     6661 ttttgtttgg gtgctccggt tactgggtat ttcatatcga gccaactgta gttgacattt
     6721 gccgaccaaa ttgaatttat ttgataagtt atgacgcttt cgaagccaaa gtttttaatt
     6781 tttcccgtat ttacgtttat tggtcttcca tttgaaatta ctgtctgaat catatttttt
     6841 ccttcaagat aaaatacagc cacatcatag aacaaatttt tttcaaccat attttgagaa
     6901 actgaaaatt cataattcat tacttcttcg ggcaataaat tggggttttg aggagtaaac
     6961 ataaaaagtt ccctgatggt tggatttctg aatcctttgc caactattgt ctttgcttca
     7021 atatttttgg ggagatgaaa ggataagccc acttgaggaa tccattgaga accggttttg
     7081 gaatgattat ctaagcgaat gcctatatcc actgtggcca gcaagccgaa gtcttgacgc
     7141 atatcagcat aacccgctat ttcgtacaag gtagtatcga taatcgattt tttatttcca
     7201 tcgttgtatt tattccatgc tttgcctcca aatttttgat agtcgaaccc aaaagtaatt
     7261 ttgttgttgg gcattaaaat tgctgattga taaaacgtta taccggatat tttatcgttg
     7321 gagtggaaaa gataatcgag tggctgtcct cccatgttgt atccatcatt tataatgtga
     7381 tttccccaat tgtaaaaaaa actcaaagct cctgaagtgt atctgtacct gttttctacc
     7441 gaagcattta tccatcccct tttaacgtgc gaatcgttgt caacaatcgg ctgatcgaca
     7501 gtgccgggat tggaagcatt gaaatgagaa atatttgcgt tggtggaaac actccagtat
     7561 ctgtccagcc gataaccgag ttttgcataa ccgcttattt ggtcgaaatc catattttcc
     7621 cggtgtccat cagtccgatt gtatgaaatg gcaaatttac cggttatccg atttttatgg
     7681 aagtgattgc ttgccgaaga ttccagcgtg ctgtaagagc cgtaaccgaa gtggaaattt
     7741 tgttgaacgg tatccttgta aaattttgat gtaataatat tgataacgcc acccatggca
     7801 ttggatccgt aaagagcaga agccgggcct ctgaccactt caatccgatc tgctatcaca
     7861 gattgataca agtctgctat gggatgtccc attaagccca taaattgtgg ttctccatca
     7921 atcagtatca atactcctgt agtaggattt cctccaattc ctctcatttt catcgttccg
     7981 gcagctcctg tcgaaattcc gtagcccata attcctcgac cggtaataaa tagtcccgga
     8041 accagttcat tcagtaacgg aattacagac tggctttggc gatcaagtat ttgtttgtcg
     8101 gtaattacgg ataagctcat gggcaaattt cttacatccg cttcacttct ggtacccgtt
     8161 acaataactt cattcatata aatggttttt tcttcatttt tcagaatact gtcccgaaaa
     8221 agttgtgctt tcagcgaaaa tgtcgaaata ctgattagaa tgaaaattgc aataaacatc
     8281 agtttacaaa agagttgttt caaattcatt attttatttc gattttgttt ataagtaaat
     8341 tcaagcaaca agtgcgaaat taaataaaat gtttgaaaaa ctcattttta ggggtattaa
     8401 agattaattt ttctcttggt cttcgattta gtttgtattg actatctttt tatatagtga
     8461 tccgaaattg tcgggatata aaaagttgtg tgcaaaaatt cttttcagag aatatccaga
     8521 cctgaaacag ggttatgctc ttacacactc cttgagaatg atttacaaca aaaacagtat
     8581 caaagccgct gc
//
LOCUS       DAOSIB010000080         8473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_7815_length_8473_cov_12.044191, whole genome shotgun sequence.
ACCESSION   DAOSIB010000080 DAOSIB010000000
VERSION     DAOSIB010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8473
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7815_length_8473_cov_12.044191"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..709)
                     /locus_tag="PLD44_07260"
     CDS             complement(194..709)
                     /locus_tag="PLD44_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP19 family protein"
                     /protein_id="HOF99164.1"
                     /translation="MIQIPSSELQQALEKDNKEFLRTIANTYLTAIGGNLNTSNMQLL
                     NGWQHVLLSWYFFSTEMDEGGFVQLIQNGYGGYIFDNPFAKALRLMGARELSKLIYRA
                     KKIYDENKTTLERDTSEEEFNAMYVDFEQFDELEEEYFDMEEEQLEIISAYVKGHLSD
                     FTEILANQVNF"
     gene            complement(724..3096)
                     /locus_tag="PLD44_07265"
     CDS             complement(724..3096)
                     /locus_tag="PLD44_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOF99165.1"
                     /translation="MKNKILFSFLCTSLFFSCQPSLHYAPDGENNFNLNSLLTLEIGE
                     DTIYLQDFILNPTEIDSITSSSSWLQWKLSNDKKLLYVKVVPEMENFVDLKIWLNGTP
                     YSIPCRKTDKTDFVFTYRPQGKAYRKVQIAGQMNDWVPNFTPDLQLNDSSYYQVTLHL
                     SPGTYLYQMVLDGEWNYDVNNPLKIDNGRGKFNSILKVEGQQSLLPELYTDAYSDSKI
                     TLLSVNKVNNIFVYWQNYRLPDMFVKKQENRIIFDIPSEAHSLERSFIRVWVSNDFGV
                     SNDVFIPLHKGKVLTDSKEITRSDKASQIIYFLMVDRFKNGNKDNDHPLRRADVHPKV
                     DFWGGDLAGIRQKIDDRYFTDLGVNTLWISPVNQNPTTPYGFYAPVPTKFSGYHGYWP
                     ISSSKVDFRFGTNNELKNLVVHAHSNNINILLDYVAHHVHEEHPLYKEHPEFFTSLYL
                     PDGTLNTERWDDQRLTTWFDTFMPTLNTTDPKVIDLMTDSALFWLKEFQLDGFRHDAC
                     KHISNDFWRELTLKIKRQNHGKNLYQIGETYGSPQLIGSFLGTGMLDGQFDFNVFDEA
                     SVAFAGIGGNSLSRVNDVLQNSFKVYGHHNLMGYISGNHDRPRFMAYASGDLKYGEDT
                     KLAGWKREIGISDSTAYDKMFLLHTFNLTIPGIPVIYYGDEIGMTGANDPDCRRMMRF
                     DHWNKREQNLWKKVSTLTHLRLQHPVLIYGDFVSLQANNDSWVYARKYFDKVAIIFIN
                     NAPKSQHFEINLPSIMTNTIYQAVFQHAFTEVNGTINIDLPAYSAEILLN"
     gene            complement(3207..5051)
                     /locus_tag="PLD44_07270"
     CDS             complement(3207..5051)
                     /locus_tag="PLD44_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOF99166.1"
                     /translation="MKKILFLLSFLLACFASKSADKKWVDHLEPTFWWTGMNQTQLQL
                     MFHGKDVALCDFSIQDKEVTIIRKELTDNPNYVFLYIDLQKVVSPKKFQIVLKKDKKI
                     QQYWYELKERKANSSLRQSFTSADAVYLLMPDRFCNGNTANDSVKGYFEGVHRAKPGA
                     RHGGDIEGIISKIPYLADLGITALWTTPLFEDNDTQYSYHHYACTDYYKIDPRLGNNT
                     DYLRLADTCHAYGLKLLMDVVPNHCGWKHWWMKDIPDKNWFNTWNNYTSSNYRITAWT
                     DPHAAEADRERLIKGWFAPNMPDLNLDFPLLFDYLRQAYVFWIEYAGIDGMRVDTYPY
                     NNIKAASQFIQSILEEYPNMNVVGECWVKTPAETAYYQSGNRNADGFDSHLSSVMDFC
                     LKDVFSLAFNEKEGWENGIVRFYSHFAQDFVYPNPNFIMIFLDNHDIDRFQTAVSNDI
                     AKYKMALAMLLTLRGYPQIYYGTEIMMPGIPGSYEGHRFDFPGGWATDQRNAFTENGR
                     TPQENEIFNYLRYLLHYRKNNTVLQTGKMKQFIPENGIYVYFRYNEEKTVMIVANNNE
                     KPAELDLQRFNEMLKQYGSAKEIFSGNFYNLKNAFSIPAKSVMIYELQ"
     gene            complement(5092..7401)
                     /locus_tag="PLD44_07275"
     CDS             complement(5092..7401)
                     /locus_tag="PLD44_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 65 glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HOF99167.1"
                     /translation="MNRYLQHDPWCIIEEGFHPEKQLASESIFSLSNGHMGQRANFEE
                     YYSGETMLGSYVAGIYYPEKAERGSWKNGYSEFNDKIVNAPNWTVISVRLNDERLDIG
                     EWDLANFKRVLNMREGYLERTFEATSFKGHRIQVTVKRFLSMAENEIGVISYTVKSLN
                     YDGRISFLPLIDGDVRNYYTNYNEPFWNVLQTRTEQDVSHLWAQTRKLDFYVCCALTY
                     DFYKNRELLNVNPIKIEKEKVAGFSVGTDIKKGETVTLRKYVSVISSLNHPREELTER
                     ACSLARQAKNKGWDELFEEHKSVWAEKWRQSDVIIDNDVAAQQAIRFNIFQLNQTYSG
                     NDPRLNISPKGFTGEKFSGATRWDTEAVCVPFYLSTSPQNVARNLLLYRFRHLPKAIQ
                     NAEKLGFTNGAALFPAATFNGSEILNEWELSFEEVYRNGIIAYAIYNYVRYTNDLDYL
                     VNYGLKVLISISRFWSQRVNISGQRKKYVILGVTGPNMYENNVNNNWFTNFIAVWCLN
                     YTMECIETIRKTNFRAFEDVAQSIGFSNEETFLWKDIVENMYFPEDKDLDIFLQQDGF
                     MDKTLTRANEIPESQRPINQHWTWDRILRSSLIKQADVVLGLYLFENFFDKETISKNF
                     DFYEPLTVHESSLSPCIHSIVACKIGKIEKGYELYLRTARLDLDDYNNEVAEGLHITS
                     MAGTWLSIVQGFGGMRIKEGLLHFYPQIPKQWQSFAFNVIFRGVHLNVRIEKSKVIIQ
                     HIKGEAIKLMIYDKSFMLLPESLIETPFE"
     gene            complement(8061..>8473)
                     /locus_tag="PLD44_07280"
     CDS             complement(8061..>8473)
                     /locus_tag="PLD44_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99168.1"
                     /translation="SADIPGRGFQSEANGYSLGNGSTTVGSTFSQKLRSNTSSQGMGS
                     GGGYYAYGKRGSSESRVSGQVSKGSMFAASELREPFSNSTPAPQRAPYAPNQGGTDPG
                     GDPLGNPIPVGEGWGVLLLLAAGYAFIMKLRAKR"
BASE COUNT         2567 a         1675 c         1572 g         2659 t
ORIGIN      
        1 tcggttgttt tttcggccat aattaagttt ttaataattg attatttttc tgaacttctt
       61 taatataaat tcactgatac aaaaatacat gaaaatgaat ggatctacaa atgtgagtga
      121 acataaatct tgaccggaag atgtttaata tttttaaaat cagtacttta tttttagtgt
      181 taaacataga attttaaaaa ttgacctgat tggcaagtat ttcagtaaaa tcggaaagat
      241 gtccttttac atatgctgaa attatttcca gttgttcttc ttccatgtcg aaatattctt
      301 cttccaattc atcaaattgc tcaaaatcaa catacattgc attaaattct tcttcggaag
      361 tatctctttc cagcgtggtt ttgttctcgt cataaatttt ttttgccctg taaatcagtt
      421 tcgacagctc acgtgctccc atcaatcgta atgctttggc aaaaggattg tcaaaaatat
      481 accctccgta accgttttga atcagttgaa caaaaccgcc ttcatccatt tcggtcgaaa
      541 aaaaatacca cgaaagcaaa acatgttgcc acccattgag caattgcatg ttggaagtat
      601 tcaaattacc accaatagct gtcaaatagg tattggcaat cgtccgaaga aattctttat
      661 tgtctttttc aagggcttgc tgcaattctg aacttgggat ttgtatcatt tttttgtatt
      721 tttctaattg agtaatattt cggcactgta ggccggcaag tctatgttga tggttccgtt
      781 aacctctgta aaagcatgtt ggaaaacagc ctgataaatt gtgtttgtca tgatgctcgg
      841 aagattgatt tcaaaatgct gggatttggg cgcattattg ataaatatta tggccacctt
      901 atcaaaatat tttcgggcat atacccagct gtcgttatta gcctgaagag aaacaaaatc
      961 gccataaatc agtaccggat gttgaagtct taaatgagtc aaagtagaaa cttttttcca
     1021 taaattttgc tctcgcttgt tccaatggtc aaaacgcatc attctcctgc aatcgggatc
     1081 attggcgccc gtcatgccaa tttcgtcgcc ataataaata acgggaatac cgggaatagt
     1141 taagttaaaa gtatgcaata aaaacatttt atcgtaagcc gtagaatctg aaattcctat
     1201 ttcccttttc catccggcca gtttggtgtc ttctccgtat ttcaaatcgc ccgaagcata
     1261 agccataaat cggggacgat cgtgattgcc tgaaatatag cccattaagt tgtgatgtcc
     1321 atacacctta aaactgtttt gcaacacatc gtttacccga cttagagaat ttccgccaat
     1381 gccggcaaaa gcaacggaag cttcatcaaa aacattaaaa tcgaattgac catccaacat
     1441 gccggtgcct aaaaaactgc caataagctg tggacttcca tatgtttcgc caatttgata
     1501 taaatttttt ccatgatttt gacgttttat ttttagagtg agttctctcc agaagtcatt
     1561 tgaaatatgc ttgcaggcat cgtgtcgaaa gccgtcgagt tgaaattctt ttaaccaaaa
     1621 cagtgctgaa tcagtcatta aatcgatcac tttcggatcg gtggtgttca aagtcggcat
     1681 aaatgtatca aaccacgtag tcaaccgctg gtcatcccat ctttccgtat tcaatgttcc
     1741 gtccggaaga tacaatgaag taaaaaactc cggatgttcc ttgtaaagtg gatgttcttc
     1801 gtgtacatga tgggcaacat aatccagtaa aatattgata ttgttggaat gagcgtggac
     1861 cacaagattt ttcagttcgt tgtttgtacc aaaccgaaaa tcgactttgg aagaagaaat
     1921 cggccaataa ccgtgatacc ctgaaaattt tgtaggtacc ggtgcataaa atccataagg
     1981 agttgtcgga ttttgattca ccggagaaat ccacaaagta tttactccca aatcggtaaa
     2041 ataccggtca tcaatttttt gtcgaatccc ggccaaatcg ccaccccaaa aatcgacttt
     2101 aggatgtaca tcagctcgac gaaggggatg atcgttatcc ttattgccgt ttttaaaccg
     2161 atcgaccatc agaaaataaa taatttggga tgctttatcc gaacgcgtaa tttcttttga
     2221 gtccgtcagt acttttccct tgtgaagcgg aatgaaaaca tcgttgctga cgccaaaatc
     2281 attggaaacc caaacacgaa taaacgaacg ttccagagaa tgagcttcgg aaggaatatc
     2341 gaaaataatg cggttttcct gtttcttcac aaacatgtcg ggcaaacggt aattttgcca
     2401 gtaaacaaaa atattgttta ccttgtttac cgataaaagt gttattttgg aatcggaata
     2461 cgcatcagtg taaagttcgg gaagcagcga ctgctggcct tcaactttca atatggaatt
     2521 gaattttccc cttccattgt cgattttaag cggattgttg acatcataat tccattctcc
     2581 atccaacacc atctgataaa gataagttcc cggacttaaa tgcaaagtta cctgataata
     2641 gctgctgtcg ttaagttgca aatcgggcgt aaagttggga acccagtcgt tcatttgtcc
     2701 agcaatttga acttttcgat atgcttttcc ctgtggtcga taagtgaaaa caaaatctgt
     2761 tttgtcggtt tttcggcagg ggatggaata aggcgttccg ttcaaccaaa tttttaaatc
     2821 tacaaaattt tccatttccg gcacaacctt aacataaagt aattttttgt cgttggataa
     2881 tttccattgt aaccaggagg aagacgaagt tatggagtcg atttctgtag gattcagtat
     2941 aaaatcctgt aaataaatgg tatcttcccc aatttccaat gttaaaagag aatttaagtt
     3001 aaaattgttt tcaccatcgg gagcataatg taaagatggc tggcaagaaa agaacagact
     3061 tgtacacaaa aaggaaaaaa gaattttgtt tttcatcaga atataattta ttttttaaag
     3121 gtctgatgga acccacatgt ctgaatatta tattcattcg tgtttgtgta tgcaatacat
     3181 gtgggtttca taacttggta acaggtttat tgaagttcgt aaatcatcac actttttgcc
     3241 ggaatggaaa aagcgttttt caagttgtaa aaatttccgc tgaaaatttc tttagcgctg
     3301 ccatattgct tcaacatttc gttgaatctt tgcagatcca attctgcggg cttttcgttg
     3361 ttgttggcaa caatcataac cgttttttct tcattgtaac ggaaatagac ataaattcca
     3421 ttttcaggaa taaactgttt catttttccg gtttgaagta cagtattgtt tttccggtaa
     3481 tgcaacaaat atcgcaaata gttgaaaatt tcgttttctt gaggcgttct gccattttct
     3541 gtaaaagcat tgcgttggtc ggtagcccag ccaccgggaa aatcgaaccg atgaccttcg
     3601 taactacccg gaatgccagg catcatgatt tccgtgccat aatatatttg tggataaccg
     3661 cgcagtgtca gtaacatggc caaagccatt ttgtatttgg caatatcatt actgacggca
     3721 gtttggaacc gatcaatatc atgattgtcc aaaaaaatca tgatgaaatt cggatttgga
     3781 taaacaaaat cctgcgcaaa atgactgtaa aaacgaacta ttccattttc ccatccttct
     3841 ttttcgttaa aggctaagga aaacacgtct ttcagacaaa aatccattac actcgacaaa
     3901 tgggagtcaa atccatccgc atttctgttg cccgattgat aataagctgt ttctgcaggc
     3961 gttttcaccc aacattcgcc cacaacattc atgttgggat attcttcgag aatcgattga
     4021 ataaattgcg aagcagcctt tatgttgttg tacggataag tatccacacg cattccgtca
     4081 attccggcat attctatcca aaaaacataa gcttgtctca agtaatcgaa aagaagcgga
     4141 aaatcaaggt ttaaatcggg catattggga gcaaaccagc cttttatgag tctttcgcga
     4201 tcggcttccg cagcatgagg atcagtccaa gccgtaattc ggtaattgga agaggtataa
     4261 ttgttccaag tattgaacca gtttttatcc ggaatgtctt tcatccacca atgtttccag
     4321 ccacaatgat tgggaactac atccatcaat aattttaatc cataagcatg acaagtgtcg
     4381 gccagtcgca aataatcggt attgtttccc agacgcggat caattttgta ataatcggtg
     4441 caggcgtaat gatgatagga atattgcgta tcgttgtctt cgaacaacgg cgtagtccac
     4501 aaagctgtga tacccaaatc ggccaaataa ggaattttgg aaataatacc ttcgatgtcg
     4561 ccaccatgac gcgctcctgg ctttgcacga tgtacaccct caaaatatcc tttcacagaa
     4621 tcgtttgccg tattgccatt gcaaaatctg tcgggcatca gcagataaac agcatctgcc
     4681 gaagtaaagc tctgccgcaa ggaagaattg gccttgcgct ctttcagttc gtaccagtat
     4741 tgctgaattt tcttatcttt tttcaggacg atttgaaatt tttttggcga aaccaccttt
     4801 tgcaggtcga tataaagaaa aacataattt gggttatcgg tcaattcttt acggataatg
     4861 gttacctcct tgtcctgaat cgaaaaatcg catagtgcca catccttccc atgaaacatt
     4921 agttggagtt gtgtttggtt catacctgtc caccaaaaag ttggttcaag gtggtcaacc
     4981 cattttttat cggctgattt tgatgcgaaa caagccaaaa gaaaagaaag caggaaaaga
     5041 atttttttca tgttggtaaa caaattattt tttaatggac ttcaatattt cttattcaaa
     5101 aggcgtttca atcaaacttt ccggcaaaag cataaaagat ttgtcgtaaa tcattagttt
     5161 gatggcttca cctttgatat gttgaatgat tactttcgat ttttcgatac gaacatttaa
     5221 atgtacccct ctgaaaatca cattgaaagc aaacgattgc cactgtttag gaatttgcgg
     5281 atagaaatgc aaaaggcctt cttttatgcg cattccgcca aaaccttgaa caatggaaag
     5341 ccaggttccg gccatacttg taatatgcag gccttcggca acttcattgt tgtaatcgtc
     5401 caaatcgagt cgtgccgtgc gcagatacag ttcataacct ttttcaatct ttccaatttt
     5461 acaggccact atcgaatgga tgcaaggaga taaggaagat tcgtgtacag tgagcggttc
     5521 gtaaaaatcg aaatttttgc ttatcgtttc tttatcgaaa aaattttcaa aaagataaag
     5581 tccaagtact acatcagctt gtttgataag cgaggaacga agtattcgat cccacgtcca
     5641 atgttgattg atggggcgct gactttcagg tatttcattg gctctggtga gtgtcttatc
     5701 catgaaacca tcttgctgga gaaaaatgtc caaatccttg tcttcgggga aatacatgtt
     5761 ttcgactata tctttccaca gaaatgtttc ttcattggag aagccaatac tttgagccac
     5821 atcttcaaaa gcacggaagt tggtttttcg tatggtttca atacattcca tagtataatt
     5881 caaacaccaa acggcaatga aatttgtaaa ccagttgttg tttacgttat tttcgtacat
     5941 gtttggtcct gttacgccca aaataacata tttttttctc tgtcccgaaa tattaacccg
     6001 ctgactccag aaacgtgaga tggaaatcag tacctttaat ccgtaattga caagataatc
     6061 caagtcgttg gtatagcgca cataattgta aatagcataa gctataatgc cgtttcgata
     6121 aacttcttca aaagacagtt cccattcgtt aagaatttcg ctgccattga aagttgcagc
     6181 agggaaaagg gctgcgccat ttgtgaaacc aagtttttcg gcattctgaa tggcttttgg
     6241 caaatgccgg aaacgataaa gtaaaagatt tcgtgccaca ttctgcggcg aagtggaaag
     6301 ataaaaaggc acgcacaccg cttcggtatc ccagcgtgtg gctcctgaaa atttttcgcc
     6361 cgtgaaacct ttcgggctga tgttcaatcg gggatcattt ccactgtagg tttggttgag
     6421 ttgaaaaata ttaaaccgta tggcttgctg tgcagctacg tcgttatcga taattacatc
     6481 ggactgacgc catttttctg cccacaccga tttgtgttct tcaaacagct catcccagcc
     6541 tttgtttttt gcctgtcggg caagggaaca ggcacgttca gtaagttctt cgcgcggatg
     6601 attcagcgaa ctgataaccg aaacatattt tctcaacgta acggtttccc cttttttaat
     6661 gtccgtacct acgctaaaac ctgcaacttt ttccttttca attttaatgg ggttgacatt
     6721 cagcaattct ctatttttgt aaaaatcgta agtgagcgca caacaaacat aaaaatccaa
     6781 tttacgggtt tgtgcccata aatgagaaac atcctgttcc gttctggttt gaagaacatt
     6841 ccagaaaggt tcgttgtaat tggtataata gtttcgcaca tctccatcta tcaagggaag
     6901 aaaagaaata cggccatcat aattcaaaga ctttaccgta taactgatga cgccgatttc
     6961 attttcagcc atgctcaaaa accgctttac agtaacctga atccggtgtc ccttgaatga
     7021 tgtggcttca aaagttcttt ccagataacc ttcgcgcatg ttcaacacac gtttaaagtt
     7081 ggcaaggtcc cattcgccta tgtccagcct ttcatcattt aaacgaacac tgatgactgt
     7141 ccaattgggg gcattcacaa ttttgtcgtt aaattcggaa tagccgtttt tccaacttcc
     7201 ccgctcagct ttttcgggat aataaatgcc ggccacgtaa gaacccaaca tggtttcacc
     7261 cgaataatat tcttcaaaat ttgcccgttg ccccatgtga ccattgctta agctgaaaat
     7321 actttcggaa gccaattgtt tctccggatg aaaaccttct tcgataatgc accaaggatc
     7381 atgttgtaaa tatctgttca tagcaattga attatgtgtt tgcttatgtg tttattccac
     7441 tgataatgta aacccgacat ttgaaataga aaccgcacga ctattccatt tttgctttca
     7501 caaatttttt caagaaaatt tttagctaat tttgtcaaac ttatatagta atgcaaatat
     7561 atttcaatta ttcgattttt cagagaaaaa aacaatattt catcagaata taatttattt
     7621 tttaatggtc tgatggaacc cacatgtctg aatattacat tcattattcg tgtttgtgta
     7681 tgtaatacat gtgggtttca tacgaaaaag atttatcaaa aaaatttaaa aacagagtaa
     7741 gataaggcaa ttgatataat gaaaattttt tgaaagcaaa atttcgattt caaaacagga
     7801 aaaagttcag tcaaaatgta attatataaa attctgtgtt cagttagaat ttttttaaac
     7861 aacacaagta gaaaaaatta aaaagtaaaa attaatgtcc ttaaaaatgt ttaggagagc
     7921 agcaacttta tttttttaca aacacattat tatacaggcg agttggaaaa atttttaaaa
     7981 aacgatttga aatcgaaata gaaagaaaaa atttcgggat aggtatgttg gagcttgata
     8041 ttcgaacatt caaacaaaaa ctatcttttt gctcttaact tcataataaa agcataaccg
     8101 gcagccagca gcaacagtac tccccatcct tcaccaaccg gtatgggatt acccaatgga
     8161 tcgccgcccg gatctgtacc accctggttt ggtgcataag gagctctttg tggagccggt
     8221 gtactgtttg aaaacggttc tctcaactcg ctcgccgcaa acatacttcc ttttgatact
     8281 tggccggaaa cacgactttc ggaagaacct cttttcccat aagcgtaata tccaccaccg
     8341 ctgcccattc cttgtgagga agtatttgat ctgagttttt gggaaaatgt ggaacccact
     8401 gttgtggatc cattgcctaa gctgtatcca tttgcttcgc tctgaaaacc tctgcccgga
     8461 atatcggcac tta
//
LOCUS       DAOSIB010000081         8340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_7983_length_8340_cov_9.979964, whole genome shotgun sequence.
ACCESSION   DAOSIB010000081 DAOSIB010000000
VERSION     DAOSIB010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8340
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7983_length_8340_cov_9.979964"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..1308
                     /locus_tag="PLD44_07285"
     CDS             79..1308
                     /locus_tag="PLD44_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOF99169.1"
                     /translation="MKKLLLLAILCFGIVSCNDETESASQLPPDAKPITLTDGQAKRV
                     GQDNEFALDLFRKVINFSKETNVFISPLSVSIALGMTWNGAAGETKSEMENTLKMGGM
                     SVEEINEYYRILQTTLPTIDPTTKLTLANSLWYRSGFQVKQDFLKVNINYFNAYVREL
                     DFSKAWAVDTINQWCAKKTNNLIPSVLDAIPDEAVMYLINAIYFKGIWRHQFEKKNTK
                     QTDFTNEEGEKVKVNMMFQKDTFAYAEDEKAQYLDMPYGNKAFSMTVILPKNGNTTAD
                     LLEYLTVDQWNSILQNLSMREVEVYFPRFSSKNKFLLNDPLIDMGMKLAFTDNADFTN
                     IANKNLFISRILHNTYIEVTEEGTEAAAVTVVEVGYTSVPIIPEFRVNKPFLFVIREN
                     STGIILFMGKMGNIELF"
     gene            complement(1350..3185)
                     /locus_tag="PLD44_07290"
     CDS             complement(1350..3185)
                     /locus_tag="PLD44_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOF99170.1"
                     /translation="MRLNFFKTIAFSGIILSVVGCANKAQGPTGGPKDSIPPSVVRSV
                     PSNGALNFKKKQILIDFDENVSIEKAAENVLISPPQVKPPEVVAIGKRVTVNLQDDLK
                     DSTTYTINFGNAIVDLNEKNPLKNFSFSFSTGNQIDTLQVKGTVINAEDLNPVSGVIV
                     GVYNDLSDTVFTQKPFLRIGKTDEIGKFSVENLKKDTFKIYALGDANRDYFYQPGEGL
                     AFTDSLIVPGFFREEVMDTIWKDSVQIDTIRSIVRTHFLPDNILLKYFKENKKRQYFI
                     KAERTLPYAFSLFFSAPMTELPTLKPIDFEWDGKYILQKNNTMDSLTYWMTDSLVWKK
                     DTLQFEMSYQKTDSLFRLVPATDTLKIFMRTARSTVRNTKDKKEPVSRVKMEQNYQFT
                     TNIAGSFDIYQPVVFRFSTPLAYFDLSKISLAQRVDTILKPLPVNWRQVDSTKMVYAV
                     DYKWEPEKNYELTVDSAAFTSIYQQTSKKYSGKFKIRSLDEYSTVIMVLENFVPNAVI
                     QILDAKDAVIASKQAQEKGTVFQYLKPGDYYARLFLDENGNGKWDTGDFAAHRQPEAV
                     FYYPKKMTLMPNWEFEETWDYLKVPLLEQKPKEIRKDAGKKVSGN"
     gene            complement(3198..4472)
                     /locus_tag="PLD44_07295"
     CDS             complement(3198..4472)
                     /locus_tag="PLD44_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOF99171.1"
                     /translation="MKESILQYVWQHKLFLQSNLQTADGEKVEVIDVGKCNTDAGPDF
                     FNSKIKIDGTLWVGNVEIHSRSSEWIKHGHQKDKAYDTVILHVVAEMDGEVFRTDGTK
                     IPQLVLPFSKDIEANYDSLLNNNKWIPCADKIEKISEIFIQSWKNALLVERLEQKMIE
                     IENLLTQNHYHWGEAFYIIIARNFGFGTNGQAFEMLAKSVPLTVLAKHKDNLLQLEAI
                     LFGQAGLLPEDTSDTYTMQLEKEYAFLRNKFSLQPIDCSQWKLFRLRPYNFPHVRIAQ
                     FASLIHQSSKLFSKIIETPNLELMKDLFSCKPSNYWQTHYLFGSASPKSTKQLGLYSI
                     YSIVINTIVPFLFCYASKKNNEDLKDKSLHLLEAIPSEKNAIVKGWRNIGIASESAFD
                     SQALIQLKKQYCDTKKCLFCRIGHKVLTLQTT"
     gene            complement(4543..5151)
                     /locus_tag="PLD44_07300"
     CDS             complement(4543..5151)
                     /locus_tag="PLD44_07300"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOF99172.1"
                     /translation="MSCQFPAFLRPLIGRNYLWRKNSSEKVVYLTFDDGPVPEVTPQV
                     LGVLDDFDIKATFFCVGDNVKKYPELYREILLKGHSVGNHTFHHLNGFKVGQEQYLKD
                     VEQASQLIDSRLFRPPYGKLTRKEKKILEQKFQIVLWDVISYDYDQNLSPSQVLKNVK
                     RYTRSGSVILFHDSVKAKNNMLTVLPQAISFLLAKGYRFEKL"
     gene            complement(5152..>6294)
                     /locus_tag="PLD44_07305"
     CDS             complement(5152..>6294)
                     /locus_tag="PLD44_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99173.1"
                     /translation="QNYLESCRPNAIIFTNGDNDTFPLWYNQEVEGKRTDIRVCNLSY
                     LQTDWYIDQMKREAYESAPLPISWEPEDYISGKNEVISVEDFIKQPIDVKTIMDFIRS
                     SDPQTKINGEAVIPTKQIYIPVDAQQVVKTGTVKPERAAEIVPKIDFNLKNRLTKSEL
                     MVIEMLKENNWERPMYFAVTVGDDYYLGLKDHFELTGMAYQIVPVGAKDAGPGVNTDE
                     MYENVMHKFRFGGINNPKVYIDENIGRMCRTHRMFMGQLAGALLAEGDTVKAKAVLDY
                     CLDMIPGTSVRHDYTSVQIANYYYRLEEFEKGDAIMDSVAKDCVEYINWGFSLSPSKQ
                     NSITGDLGNNMAVLSQILQICDELKRKNILDKYLPVYMQYSRYFQM"
     assembly_gap    6295..6394
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6395..8223)
                     /locus_tag="PLD44_07310"
     CDS             complement(<6395..8223)
                     /locus_tag="PLD44_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOF99174.1"
                     /translation="MNDSMKRFKSLNVIFGWVAFVIATATYLLTLEPTASFWDCGEFI
                     STAYKLDVGHPPGAPFFMLTARFFTLFTSDPTRVAVMVNTLSALCSGLTILFLFWTIT
                     HLARKVVIKSDEDYTTGNIIIILGAGLVGALAYAFSDTFWFSAVEGEVYAYSSLFTAV
                     VFWLILKWEETADEPGSDRWLLLIAYLMGLSIGVHLLNLLAIPAIVLVYYFKKFTPSF
                     KGVLLALLGSVVILAVVLYGIIPGFVQVASWFELLFVNKLGMAFNSGLYVYIILSIAA
                     LTWAIYESYVNKNYFRIVLSFLVAITLVGIPFFGQHIVLGIILVAGLSFYFYKWKNKI
                     NVHWIHTSILMITLMLIGYSTYSVIVIRSAANPPMDQNSPDNVFSLRYYLNREQYGDR
                     PLIYGPVYNAPVKLRIDGNMCIPEQELGEAMYAPKPKKSPNEKDQYMLTGYKMDYKMD
                     ERFNMLFPRMYSTNSSHVQAYKIWGNVKGKPITFDYCGQQRTEMKPTFAENLRFFFDY
                     QLNFMYWRYFLWNFSGRQNDLQGNGEVDKGNWITGINFIDKYLVGDQTNLPTFLAENK
                     GRNAYFMLPLILGILGILWQLYGGKNGKQTFWVTFLLFFMTGIA"
BASE COUNT         2614 a         1695 c         1457 g         2474 t
ORIGIN      
        1 cttgtgaaac ttaacatgaa ttgccgatta ttccttaaat tgtagcttat attcttttca
       61 tttcaaaaga ttttagccat gaaaaaactt ctactacttg ccattctatg tttcggaatt
      121 gtttcatgca acgacgaaac tgaatctgcc tcacaacttc cgcccgatgc aaaacctatt
      181 actttgaccg acggacaggc aaaacgtgta ggacaagaca atgaatttgc tctcgattta
      241 tttcgtaaag tcatcaattt ttcaaaagaa acgaatgttt ttatttctcc cttaagtgtc
      301 agcattgcac tgggaatgac gtggaacgga gcagccggcg aaaccaaatc cgaaatggaa
      361 aacactctga aaatgggtgg catgtcagtt gaagaaatca atgaatatta tcgtattttg
      421 caaaccacgc ttccaaccat tgacccgacc accaaactca ctcttgccaa ttctttgtgg
      481 tatcgcagcg gttttcaggt aaaacaggat tttttgaaag ttaatatcaa ttattttaat
      541 gcgtatgtac gagaactcga tttttcaaaa gcttgggcgg tggatactat taatcaatgg
      601 tgtgctaaaa aaaccaataa tttgattcct tcggtactgg atgcaattcc cgatgaagca
      661 gtgatgtatc tgatcaatgc catttatttc aagggaatct ggcgtcatca atttgagaag
      721 aaaaacacta agcaaactga ttttaccaac gaagaaggcg aaaaagtgaa agtgaacatg
      781 atgtttcaga aagatacttt tgcctatgca gaagatgaaa aggctcaata cctcgatatg
      841 ccttacggaa acaaagcttt cagcatgacg gtaattttac cgaaaaatgg aaatacaacg
      901 gcagatttgt tggaatatct tacagtagat cagtggaaca gcattcttca aaatttatct
      961 atgagagaag tagaagttta ttttccacga ttcagcagta aaaataaatt ccttttaaac
     1021 gacccgctga ttgacatggg catgaaattg gcatttaccg ataatgccga ttttacaaac
     1081 attgccaaca aaaatctttt tatttcgaga attctccaca atacttatat cgaagttacg
     1141 gaagaaggaa ctgaagctgc ggctgtaact gtagtagaag ttggatatac ttccgttccc
     1201 atcattcccg aatttcgagt aaacaaacca tttttatttg tgattcgcga aaacagtacg
     1261 ggtatcattc tctttatggg caaaatgggg aatatagaat tgttttgatg agtgatgttc
     1321 tgaaaatgat tcggcaacct ttcttcatct caatttcccg ataccttctt tccggcatct
     1381 ttgcgaattt ccttgggctt ctgttcaagc agaggcactt taagataatc ccatgtttct
     1441 tcaaattccc aattgggcat tagagtcatt tttttcggat aatagaaaac cgcttccggc
     1501 tgcctgtgag cagcaaagtc tccggtatcc catttgccgt ttccattctc atctaaaaac
     1561 agtcgagcat aataatcgcc gggtttcaga tactgaaaaa cggttccttt ttcctgtgcc
     1621 tgctttgaag caatcaccgc atctttggca tccaaaattt gaatcacggc gttgggaaca
     1681 aaattttcca ataccataat aaccgtgctg tattcatcca aagaacgaat tttgaatttt
     1741 cctgaatatt tcttactggt ttgctgatat atgctggtaa aagcagccga atcgactgtc
     1801 aactcataat ttttctccgg ttcccatttg taatcgacag cataaaccat tttggtagaa
     1861 tctacctgtc gccaatttac aggcaaaggt tttaaaatcg tatcaactct ttgagcaagt
     1921 gaaattttgg ataaatcaaa ataagccaga ggcgtactga aacgaaaaac gacaggctgg
     1981 taaatatcaa aagaaccggc aatattggtt gtaaactggt aattttgttc catttttacc
     2041 cgtgaaacag gttctttttt gtctttggta tttctcacag tagagcgagc agttcgcatg
     2101 aaaattttca atgtatccgt tgcaggtaca agtcgaaaaa gcgaatcggt tttctgataa
     2161 ctcatttcaa actgcaacgt gtctttcttc caaaccaacg aatcagtcat ccagtaagtc
     2221 agactgtcca tcgtattgtt tttctgaaga atatatttgc catcccattc aaaatcaatt
     2281 ggtttcaagg ttggcaattc ggtcatgggt gcactgaaaa acaaagaaaa agcataaggt
     2341 aaagttcgtt cagctttaat gaaatattgt ctttttttgt tttccttaaa atatttcagc
     2401 aggatgttat caggcaaaaa atgcgttcgt acaatggagc gaatggtatc gatttgaaca
     2461 gaatctttcc aaatcgtatc catcacttct tctctgaaaa atccaggaac aatcagcgaa
     2521 tcggtaaaag caagcccttc acccggctga taaaaataat ctctgttggc gtcgcccaaa
     2581 gcatatattt tgaaagtatc ctttttcaaa ttttctactg aaaacttccc gatttcatcc
     2641 gttttcccaa ttctcaaaaa aggtttttgt gtaaaaaccg tatcggataa atcattataa
     2701 acaccaacaa taactcccga aaccggattc aaatcttccg cattgataac agttccctta
     2761 acttgcaagg tatcgatctg atttccggta gaaaacgaga aagaaaaatt ttttaatgga
     2821 tttttttcgt tcaaatctac tatggcattc ccgaaattaa tagtgtaggt tgtactgtct
     2881 tttaaatcgt cctgcaaatt gaccgtaact ctctttccga tggcaacaac ttcgggcggt
     2941 ttcacctgag gaggagaaat cagtacattt tcggccgctt tttctatact gacattttca
     3001 tcaaaatcga tcagaatttg ctttttctta aaatttaacg caccgttgga agggacagag
     3061 cgaacaacac ttggcggtat ggagtctttt gggccgccgg ttgggccttg tgctttgttg
     3121 gcacagccaa caaccgataa aattattcct gaaaaagcga tcgtcttaaa aaaatttaat
     3181 ctcattgttg tttcaaatca ggttgtttgc aacgttaata ctttgtgccc aattcggcaa
     3241 aacaaacatt tctttgtatc gcaatattgc tttttcagtt gtataagagc ctgagaatca
     3301 aatgctgatt cggaagcaat tccaatattt ctccaacctt tgacaatggc atttttttct
     3361 gaaggaatgg cttccagcaa atgcaaagat ttatctttta aatcttcgtt gttctttttt
     3421 gaagcataac aaaacaaaaa gggaacaatc gtattgatta caatgctgta gatagaatac
     3481 aatccgagct gtttggtgct ttttggactt gcagagccaa acagataatg cgtttgccaa
     3541 taattggaag gtttgcagga aaacaggtct ttcatcaatt ccaaattggg agtttcgata
     3601 atttttgaga aaagctttga tgactgatga atcagggaag caaattgtgc aattctaaca
     3661 tgaggaaaat tataaggcct gagtctgaat aatttccatt ggctgcagtc gatgggctgt
     3721 aacgaaaatt tgtttctcaa aaaagcatat tccttttcca attgcatggt ataggtatcg
     3781 gaagtatctt caggcagcaa acctgcctga ccaaacaaaa tggcttccaa ttgcaacaga
     3841 ttatccttat gttttgccaa aacagtcaat ggaacagact ttgccaacat ttcgaaagcc
     3901 tgaccattgg tgccaaaacc aaaatttcgc gctataataa tgtaaaaagc ttctccccaa
     3961 tgataatgat tttgtgtcaa caaattttct atctctatca ttttttgttc caatcgctcc
     4021 accagcaaag catttttcca actttgaata aaaatttcgg aaattttctc aattttatct
     4081 gcgcaaggaa tccatttatt gttgttcaac aacgaatcgt aattcgcttc aatatctttt
     4141 gaaaatggta aaaccagctg aggaattttt gttccgtccg ttctgaagac ttctccatcc
     4201 atctctgcca ccacatgcaa aattacggta tcataagctt tatctttctg gtgtccgtgt
     4261 tttatccatt ccgaagaacg ggagtgaatt tccacattac ccacccaaag ggttccgtcg
     4321 atttttattt tagaattaaa aaaatcgggg cctgcatccg tattgcattt acccacatca
     4381 atgacttcca ctttttctcc atcagcagtt tgcagattac tttgtaaaaa aagcttatgc
     4441 tgccatacat attgcaaaat agattctttc atggaaggga ttttcagtta agaaatacag
     4501 acttttgaat caataaatta ttgagaaaaa acaaaaaata catcagagtt tttcgaaacg
     4561 ataacctttt gccaacagaa aacttatggc ttgtggaaga accgtcagca tattgttttt
     4621 tgccttcacg gaatcgtgaa acaaaatcac cgaaccactt cttgtatagc gtttcacatt
     4681 cttcaaaact tgtgaaggcg agagattttg gtcatagtca tacgaaatca catcccacaa
     4741 gacaatctga aatttttgtt ccaaaatttt cttctctttg cgagtgagtt ttccgtaagg
     4801 aggacgaaac aaccggctgt caatcaactg cgaagcctgt tccacatctt tcagatattg
     4861 ctcttgccca actttaaacc cgttcaaatg atgaaaagtg tgattcccaa cgctgtgccc
     4921 cttcaaaaga atttcccggt aaagttcggg atattttttc acattgtcgc ctacacaaaa
     4981 aaaagtagct ttaatgtcaa aatcatcaag tacaccaagt acctgaggcg taacctcagg
     5041 caccggtcca tcatcaaaag tcagatagac aactttttcc gaagaatttt ttctccacaa
     5101 ataatttcgt cctatcagcg gacgcaaaaa agcaggaaac tgacagctca ttcacatttg
     5161 aaaataccgc gaatattgca tgtaaacagg aagatactta tcaagaatat ttttccgttt
     5221 tagctcatcg caaatttgca gtatctgact tagcactgcc atgttatttc ccaagtctcc
     5281 cgttatgctg ttttgttttg aaggacttaa actaaaaccc caatttatat attctacaca
     5341 atctttagcc acgctatcca tgatggcatc acctttttca aattcttcca ggcgataata
     5401 ataatttgct atttgcacgg aagtataatc atgacgcaca gaggttccag gaatcatatc
     5461 caaacaataa tcaagcactg ctttagcttt tacggtgtcg ccttcagcaa gtaaagcgcc
     5521 agccagctgt cccataaaca tgcggtgtgt gcgacacatt ctgccaatgt tttcatctat
     5581 atacactttg ggattattta ttcctccaaa ccggaattta tgcatcacat tttcgtacat
     5641 ttcatccgta ttaacaccag gacctgcatc cttcgcacct acgggcacaa tttgataagc
     5701 catacctgtg agctcaaagt gatcttttaa tcctaaataa taatcatctc ctactgttac
     5761 ggcaaaatac atgggacgtt cccaattatt ttccttcaac atttcaatca ccatcaattc
     5821 gctttttgta agcctgtttt tcaggttgaa atcaattttt ggaacaattt cggctgctct
     5881 ttcaggtttc acggttccgg tttttactac ctgctgtgca tcaacgggaa tgtaaatttg
     5941 cttggtcgga ataaccgctt caccatttat tttggtttga gggtcggaac tccgaataaa
     6001 atccatgatg gttttaacat caataggctg cttaataaaa tcttccaccg aaatcacttc
     6061 atttttaccc gaaatataat cttcaggttc ccaagaaatg ggtagaggag ctgattcgta
     6121 agcttcacgt ttcatctgat ctatgtacca gtcggtttgc aaataactta aattgcaaac
     6181 tcttatgtcg gttcttttcc cttccacttc ctgattgtac cacagaggga aagtatcatt
     6241 atcgccgttg gtgaagatga tggcattcgg tcggcacgat tcaagataat tttgnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcaatc ccggtcataa agaataagag
     6421 aaaggtaacc caaaaagttt gcttaccatt ttttccaccg taaagctgcc ataaaattcc
     6481 aagaataccc aaaatgaggg gaagcataaa ataagcattt cttcctttat tttctgcaag
     6541 aaaagtagga agattcgttt gatctccaac cagatacttg tcaataaaat tgataccggt
     6601 aatccagttt cccttatcaa cttctccatt tccctgcaag tcgttctgcc ggccggaaaa
     6661 attccaaaga aaatatctcc aatacatgaa atttaattga taatcgaaaa agaaacgcag
     6721 attttcggca aaagtaggtt tcatttctgt gcgctgctgt ccacaataat cgaaagttat
     6781 gggtttaccc tttacattgc cccatatttt gtatgcctga acatgcgaac tgttggtgct
     6841 gtacatacgt gggaataaca tattgaacct ttcgtccatc ttatagtcca ttttataccc
     6901 tgtaagcata tactgatctt tttcgttggg cgattttttt ggttttggtg catacattgc
     6961 ttctcccaac tcttgttcag gaatacacat attaccatca atgcgcaact tcaccggtgc
     7021 gttgtaaaca ggtccataaa tcagtggccg atcgccatac tgctcacgat tcaaataata
     7081 acgcaacgaa aaaacgttgt cgggtgaatt ttgatccata ggcggattgg cggcagaacg
     7141 aataacaatg acgctataag tcgaatagcc aatcaacatt aaagttatca tcagaatgga
     7201 agtatgaatc caatgaacat tgattttatt tttccattta taaaaataaa agcttaagcc
     7261 ggcaacaaga ataatgccca gcacgatatg ctgtccaaaa aacggaatgc ctactaaagt
     7321 aatggccacg aggaaagaca atacaatcct gaaatagttt ttgttaacat aagattcgta
     7381 aatagcccaa gtcagtgcag ctatcgaaag aataatgtaa acataaagcc ctgaattgaa
     7441 agccattccc aatttattga caaacagaag ctcaaaccag ctggcaacct gaacaaatcc
     7501 gggtataatt ccatacaaaa caaccgccaa aatcacgact gatccgagca atgccaatag
     7561 aacgccttta aaggatggtg taaatttttt gaaataataa accagcacaa tggcaggaat
     7621 tgccaacaaa tttaaaaggt gaacaccaat agacaaaccc attaaatagg caatcagtaa
     7681 caaccatcga tccgaaccgg gctcgtcggc agtttcttcc catttcagta tcagccaaaa
     7741 aactactgct gtaaacagtg aagaataagc atacacttct ccctcgacag ccgaaaacca
     7801 gaatgtatcg gaaaaagcat aagccaaagc tccaaccagt ccggcaccta agatgataat
     7861 aatgttgccg gtagtataat cttcgtccga cttaatcaca accttgcgag ccagatgagt
     7921 aatagtccag aaaaggaata aaatagttaa gccactgcac aaagccgaca aagtatttac
     7981 cataactgcg acgcgtgtgg gatcggaagt aaatagcgta aaaaatcttg ccgtcagcat
     8041 aaaaaacggc gctccgggag gatgacccac atccagcttg tacgctgtgg atatgaattc
     8101 gccgcaatcc caaaagctgg cggttggctc caaagttagc aaatacgttg cagtagcaat
     8161 aacaaatgct acccagccaa aaataacgtt gagagatttg aatcgtttca ttgaatcatt
     8221 cattgaaaaa ttttttagct ttttgctgaa tatataagag actccaaagg tacaaagaaa
     8281 atttgtttta aaaagacgaa atgatttccc cgcttctttt taaaattgaa acaaaaaaaa
//
LOCUS       DAOSIB010000082         8168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_8188_length_8168_cov_9.579194, whole genome shotgun sequence.
ACCESSION   DAOSIB010000082 DAOSIB010000000
VERSION     DAOSIB010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8168
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8188_length_8168_cov_9.579194"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..2637)
                     /locus_tag="PLD44_07315"
     CDS             complement(118..2637)
                     /locus_tag="PLD44_07315"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOF99175.1"
                     /translation="MPEEYKETISPSDLVLRLSEKANNFDISKYEDFIAELCGEREYQ
                     IEATRRVIKFFLSGEYKNIGELVSENFKTNEAMQEFFRDEGELLGDLLFRDKLSCTID
                     LATGTGKTWVMYAVARIMLAEGVVDNVLIVGPSRTIKDEIWKKFNNFTIKTNLQSAFP
                     KDGKYRNPGIIKANTAIKKGDIVIDNIHKAYDHVGSSIKDLRGKDRKILIINDEAHHF
                     INSTAGGLDKEEMLEWAKFLRNPEYNFAYILNSSGTPYKGDNYFKDVIYRYPIRQAIE
                     DRMVKDINYLEKDESRGWKQKFRAIYRNHIENKREYPKVKKHITIFVTNQISKTDKIA
                     DEIRKFLRTEEGISAEEAKKKVLPVTSAQKHKENRELLKTVDEPENPVEWIVSVSMLT
                     EGWDVNNVFQIVPHEDRAFNSKLLIAQVLGRGLRVPPVYQNDAKIHPQVTIFNHDAWG
                     PRIDDLVRDVAEISKKITSKVDKESKYNFSVYWMNVEKKIVSDSKTPARGKIELPKSL
                     GFSSRKDITEQIYRSVRTREETIKRTKIDLEEYSIKEAVNEVFNNILLLDLTHNTNFA
                     EKANRKFIENLIEKELEDIGEKTVTEANLQRAKSSFNTLYRQLTGQSRIVDVYSDPIE
                     KTTKEMNSSFISISEVKKNKGIMFSEESLKSSTAKELENINEALDEIKGKYSIKIDED
                     DYKSPLNVTVLASEPEIDFAKILTRKENAKFIDCWVKSKDKGFYSIPYNYRPGTHPQQ
                     KQFNPDFIIKKGGRIIFVETKHDEDTDVKNRDKIAGANDYFMELNAKVEKEGQEYCFY
                     FLTPADYTGFFEKVIRNNKTFIGELHAELLKKSREELKELV"
     gene            complement(2639..4495)
                     /locus_tag="PLD44_07320"
     CDS             complement(2639..4495)
                     /locus_tag="PLD44_07320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOF99176.1"
                     /translation="MKKLTKQQKEEIIKRIQENKPIPDEYRFLIPFETEREYELTYAD
                     KERKEDILADTLSVPFQPIKKFGETKENEWHNMLIFGDNLQALKHLLKLKEQGKLRNP
                     DGRNGVKLVYIDPPFATEQEFRGAKEQKAYQDKIAGAEFLEFLRKRLIFLKELLTEDG
                     SIYVHLDWRKGSHIRLLMDELFGENNFRNEIIWHYETGGVGKTEYAKKHETILFYTNS
                     NNYIFHPERSKDPRTEKALKRAQIPAGARISASDTTKLPMDVWLIQALNPVENERLDY
                     PTQKPERLLDRVIKVSSNPGDIILDCFAGSGTTGAVAEKLGRKWIMIDSSKLAIYTIQ
                     KRMLNLKAEIGNKGRALKPKPFVLYNAGLYQDSGFIEKMDEADYRKFVLELFGAEPSD
                     NKVKGVQMHGILNNRPVMVFSQKDYLTYEFIDDLHKTIGSGLKDEMYIIAPQSVVRFN
                     EDYVDKGRIRYYVLRVPYSIIQKIQEKGFIRGLQPLSKANINQTIESVGFDFIYPPKV
                     ECEYYQGKDGGLFDQLVIKIKKFEPVQISKKPVEFEDPKKEALSMVMIDENYDGKVFD
                     MDHYFFGDEIVKNDFKVVFPKEKAGQKLMIIYLDVLGNEKVEVKNIKDFKKK"
     gene            complement(4499..5215)
                     /locus_tag="PLD44_07325"
     CDS             complement(4499..5215)
                     /locus_tag="PLD44_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99177.1"
                     /translation="MKKDLLEKIKKRGYWRINFQPLIDTQKFTSLGECKDIVEKNTVE
                     LRGWDYPHFPRRRGDDTGLEPGENFYQGWVDWENHKEFWRMYQSGQFIHYLALREDWL
                     EEDSWQQNLHEKIKPMSSLGIIGSVVYQMTEIFEFLSRLGTAGIYDEGVRVSIALHNT
                     ENRELWIEDSMRAPFFQPYKTGAQKIEFVKEFTKEQIITSPKDLAFEVIKYIFDRFGW
                     HNPPIETIKKDQDNLLSGRI"
     gene            complement(5212..5781)
                     /locus_tag="PLD44_07330"
     CDS             complement(5212..5781)
                     /locus_tag="PLD44_07330"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF99178.1"
                     /translation="MTTGELEAKLEGGTETQTIEFKGACNWDDKIFAKDILAMSNVQD
                     GGFIIVGMKELDDGTFEREGITEEIRASYKIDEMKDQMTKYADPHVNFVVDFPKDRNG
                     KEYAVIRVFQFEEVPVICRKDSTDTKAGVVYYRNKNRRVESAPVSNSYDMRDIIKIAT
                     VKMMQRKTELGFTVKQSVKKKLDDELKGL"
     gene            complement(5786..5995)
                     /locus_tag="PLD44_07335"
     CDS             complement(5786..5995)
                     /locus_tag="PLD44_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOF99179.1"
                     /translation="MSKEISPIGKNIKRLRKQKGLSQDRLSKLADISYNTVIKLESGG
                     ITNPSIDTLQKLAKALDVSVDNLLK"
     gene            6047..6562
                     /locus_tag="PLD44_07340"
     CDS             6047..6562
                     /locus_tag="PLD44_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99180.1"
                     /translation="MIQNYFDNVKTFLGSLIPNTRAREGWGKGIYLFSFLKIISSPAK
                     RAWRAGKKKRGSGGNEFLPARPFWVVGGCASPSATHKSEFSVGVLFKISSDFVQKVPP
                     FFRIRIFSDCCLALATLGLGKNEDFIGIFEIQKQFLPEQTEVRFAKGKHKFEKFINQS
                     FFFRRRRKAKR"
     gene            6590..>8168
                     /locus_tag="PLD44_07345"
     CDS             6590..>8168
                     /locus_tag="PLD44_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOF99181.1"
                     /translation="MTNKNNQTKKFFIYARKSTDTEDRQVRSIQDQIAELKELAKKEN
                     LEVVDILVEKQTAKKPGRPVFAEMLKRIEAGEAEGILAWHPDRLARNSVDGGQIIYLV
                     DTGIIKELKFPTFWFENTPQGKFMLSIAFGQSKYYIDNLSENIKRGHRQKLKNGLWPQ
                     MAPLGYLNNKETKQIYVDKEKAPLIKKTFELYATGKYTLKEIRKIINDLGLKGRRGKM
                     LSVSNYQYILQNPFYYGLIRYNGEYYEGKHEPIIAKKLFDLCQEVMKRKSKPQKADKM
                     KFFLYRGLFKCGECGYTITADRKIKKSGKSYTYYYCTKKNPNHKCSQNVFTREEKISS
                     QIKEVIQKVSLPDDWAEKMLNELEKEKNETAQSSRFFAQKIQYEIKIIDEKLEKLMNA
                     YLENALSLDEYKEAKNKLVNQKQLLKDKLNAFEQKSNNRFELAAKFINTVKQAEIIAL
                     QKNPEQSRDFLKKIGSNFRLSGQKLFLDFKNPFKILAEAEPRLWRGEATIAQNPNSEN
                     WRCLLVKIRTFFDENPDC"
BASE COUNT         2296 a         1786 c         1379 g         2707 t
ORIGIN      
        1 cgcacaccca cccaccaccc aaaacgggcg ggcgggcaaa aattcatttc ccccaaaccc
       61 ccttttcttt ttgcccgccc gccttgcccg cttcgcgggc gagccgatga ttttttatta
      121 taccaattct ttcaattcct ctctactctt cttcaataat tctgcatgta attctccgat
      181 gaaagttttg ttatttctta tcactttttc aaaaaatccg gtatagtccg ctggcgttag
      241 aaaataaaag caatactcct gtccctcttt ctcaaccttg gcgtttagtt ccataaaata
      301 atcatttgcg ccggcgattt tatctcggtt ttttacatcc gtatcctcat cgtgtttcgt
      361 ttccacaaaa ataatcctgc cccctttttt aataatgaag tcggggttaa attgtttttg
      421 ttggggatgc gtgccggggc gataattata cgggattgaa taaaatccct tgtcttttga
      481 tttcacccaa caatcaataa atttcgcgtt ctcttttctt gttagtattt tcgcaaagtc
      541 aatttccggt tctgaagcaa gcaccgtaac attcagcggc gatttgtaat catcttcatc
      601 tatttttata gaatacttgc cttttatctc atcaagcgct tcgttgatat tttctaactc
      661 ctttgccgta gatgatttaa gagattcttc gctaaacatt attcccttgt ttttctttac
      721 ctcgctgatg ctaataaagg acgaattcat ttctttcgta gttttttcaa tcggatcgct
      781 atacacatcc actattcgag attgaccggt taattgtctg taaagcgtat tgaaagacga
      841 tttagctcgc tggagattgg cttctgttac cgtcttttcg ccaatatctt ccagctcttt
      901 ttcaatcaag ttttcaataa attttctatt cgctttctca gcaaagttag tattatgcgt
      961 caaatccaaa agcaggatat tgttaaaaac ttcattcacc gcttctttga tggaatactc
     1021 ctctaaatca atttttgttc gtttgattgt ttcctctcgc gttctgactg atctataaat
     1081 ctgttcggtt atgtcttttc tgctgctaaa tccaagagat ttcggcaatt caatttttcc
     1141 gcgagcagga gtttttgaat cggaaacaat cttcttttca acattcatcc aataaactga
     1201 gaaattatat tttgattctt tatcaacttt gctggtaatt ttcttgctga tttccgccac
     1261 atcccgcacc aagtcatcaa tccttggccc ccaagcgtcg tgattaaaaa tcgtaacctg
     1321 cgggtgaatt ttcgcgtcgt tttgataaac tggcggcacg cgcaagcctc tgcccaatac
     1381 ttgggcaatc agcaattttg aattaaacgc tctgtcttcg tgggggacaa tttgaaaaac
     1441 attatttaca tcccaaccct cggtaagcat agaaacagaa actatccact ctaccggatt
     1501 ttccggctcg tcaacagttt ttaataattc ccggttttct ttatgctttt gagcagaagt
     1561 aactggcaat acctttttct ttgcttcttc ggccgagatt ccttcttccg ttctcaaaaa
     1621 cttcctgatt tcatcagcaa ttttatcggt ctttgaaatc tggttggtaa caaaaatcgt
     1681 gatgtgcttt ttaactttcg gatattccct tttattttca atatggtttc tgtaaatcgc
     1741 tctaaatttc tgcttccagc cccttgattc gtctttttct aaatagttga tgtctttcac
     1801 cattctgtct tctatcgctt gccggatcgg atacctgtaa ataacatctt tgaaataatt
     1861 gtcgcctttg tacggcgttc ctgacgagtt aagaatataa gcaaagttat attccggatt
     1921 tcttaaaaac tttgcccact ctaacatttc ttctttgtct aatccgccgg cagttgaatt
     1981 gatgaaatga tgggcctcat cgttgattat caaaattttt ctgtcttttc ctcttaaatc
     2041 ttttattgat gagccgacat ggtcatacgc cttatggata ttatcaatca caatatcgcc
     2101 ctttttgatc gctgtatttg ccttaataat gcccgggttt ctgtattttc catctttggg
     2161 aaaggcgctt tgcagatttg ttttgattgt aaaattatta aactttttcc aaatctcatc
     2221 tttaattgtt cgcgatggtc caacaatcaa aacattatca actacgcctt cggcaagcat
     2281 tatccttgct accgcataca tcacccaagt tttcccggtt ccagtggcta aatcaatcgt
     2341 gcaacttaat ttatctctaa aaagcaaatc gccaagcaat tcgccttcgt ctctaaaaaa
     2401 ctcctgcatt gcttcgttcg tcttaaaatt ctcgctgact aattctccaa tgtttttata
     2461 ctcaccgctt aaaaagaatt tgataaccct tcgcgtcgcc tcaatctgat attcccgctc
     2521 gccgcaaagc tcagcgataa aatcctcgta tttagaaatg tcaaaattgt tggctttctc
     2581 tgaaagccgt aaaactaaat cgctcgggct gattgtttct ttgtattctt cgggcatatc
     2641 actttttctt aaaatccttg atatttttca cttccacttt ctcgtttccc aaaacatcaa
     2701 gataaataat cattagcttt tgtccggctt tctctttagg gaaaacaacc ttgaaatcgt
     2761 ttttaacaat ctcatcgcca aagaaataat gatccatatc aaaaactttt ccgtcgtaat
     2821 tttcgtcaat catcaccatt gataacgctt cttttttcgg gtcttcaaat tccaccggct
     2881 ttttgctgat ttgtaccggc tcaaatttct ttatctttat caccagctgg tcaaaaagcc
     2941 cgccgtcttt gccttgatag tattcgcact ccacctttgg cggatagata aaatcaaagc
     3001 cgacgctctc tatcgtctga ttgatatttg ctttggataa aggctgaagt cctctgataa
     3061 aacctttttc ttgaattttc tggataatgg aataaggcac tcttaaaaca tagtaccgga
     3121 tccttccttt atccacataa tcttcattga acctgacaac gctttgcggc gcaataatat
     3181 acatctcatc ttttaatccg cttccgattg ttttgtgtag gtcatcaata aattcataag
     3241 tcaaataatc tttttgtgaa aacaccatca ccggccggtt attcaaaatg ccgtgcattt
     3301 gaacaccttt aactttgttg tcgcttggct ccgcgccaaa gagttccaaa acaaattttc
     3361 tgtaatccgc ctcgtccatt ttctcaataa atccggagtc ctgataaagc ccggcattat
     3421 acaaaacaaa cggctttggc tttaatgctc tgcctttgtt gccgatttct gctttcaaat
     3481 tcagcatccg cttttgaatt gtataaatcg ctaactttga agaatcaatc attatccatt
     3541 tcctgcccaa tttttccgcc accgcgccgg ttgtgccgct tccagcaaag caatctaaaa
     3601 taatgtcgcc ggggttagaa gaaactttta tcactctatc aagtaaacgc tctggttttt
     3661 gtgttggata atctagtcgt tcattttcta cgggattaag tgcctgaatc aaccaaacat
     3721 ccatcggaag tttagtagtg tctgaagcag atattctagc accagctggg atttgagctc
     3781 tttttaatgc tttctctgtt cgtggatctt ttgatctttc tggatggaaa atgtaattgt
     3841 tgctatttgt gtaaaacaaa atagtttcat gtttcttggc atattctgtt tttccaacac
     3901 cgcctgtttc ataatgccaa atgatttcat ttctgaaatt attctcccca aataattcgt
     3961 ccattaaaag tctaatatga gatcctttcc gccaatctaa atgcacataa atacttccgt
     4021 cttcggttaa caactctttc aaaaatatca gccgctttct taaaaactcc aaaaattccg
     4081 ctccagcgat tttatcttga taggcttttt gttctttcgc tcctctaaac tcctgctctg
     4141 tcgcaaatgg cggatcaata taaacaagtt ttacgccgtt tctgccgtca ggatttctta
     4201 attttccttg ctcttttaat ttcaaaaggt gctttaatgc ttgtaaatta tcgccgaaaa
     4261 tcagcatatt atgccattcg ttctcttttg tttcgccaaa tttcttaatc ggctgaaacg
     4321 gcacgctcaa agtgtctgcc aaaatatctt cttttctttc cttatcggca taagtcaatt
     4381 catattctct ttccgtctca aacggaatca aaaaccgata ttcatccgga atcggcttgt
     4441 tttcctgaat ccttttaata atttcctctt tttgttgttt agttagtttt ttcatagatt
     4501 aaattctgcc acttaataaa ttatcctggt ctttttttat tgtttcaatc gggggattat
     4561 gccagccaaa cctatcaaaa atatatttta ttacctcaaa agcaaggtct ttcgggctgg
     4621 ttataatctg ttccttggta aattctttaa cgaattctat tttttgtgcc cctgttttgt
     4681 atggctgaaa aaatggcgct cgcatgctgt cctcaatcca gagttcccta ttttccgtat
     4741 tatgcaaggc gatagaaacg cgcacgccct catcgtatat gccggctgtt ccaagccgcg
     4801 atagaaattc aaaaatttcg gtcatttgat atacaacact gccgataatt ccaagcgagg
     4861 acattggctt gattttttca tgcaagtttt gttgccaact atcttcttca agccaatctt
     4921 cgcgaagcgc aagataatga ataaattggc cgctttgata catccgccaa aattctttat
     4981 ggttttccca atctacccat ccttgataaa aattttctcc gggctctaat cccgtatcat
     5041 ctcccctacg tctcggaaag tgggggtagt cccatccgcg aagctcaact gtattttttt
     5101 caactatatc cttgcattcg ccaagagaag taaatttttg agtatcaata agcggctgga
     5161 aatttatccg ccaataaccc ctttttttga ttttttctaa taaatctttt ttcataatcc
     5221 ctttaattca tcgtctaact tttttttaac cgattgctta acagtaaagc caagttctgt
     5281 tttcctttgc atcatcttta cggtggcaat ttttatgata tctctcatat cgtaagaatt
     5341 ggacactggc gcgctttcaa cccttctgtt tttatttctg taataaacta ctcctgcttt
     5401 tgtatcggtg ctatcttttc tacaaatcac aggcacttcc tcaaactgaa aaactcgtat
     5461 aacagcgtat tccttgccgt ttctatcttt gggaaaatca accacaaaat ttacatgggg
     5521 atcagcgtat ttcgtcattt ggtccttcat ttcgtcaatt ttgtacgatg ctctaatttc
     5581 ctctgttatt ccttctcttt caaaagttcc gtcgtccaat tctttcattc ctacgattat
     5641 aaaaccgccg tcttggacgt ttgacatggc caaaatatct ttggcaaaga ttttatcatc
     5701 ccaattacaa gcgcccttaa actcaatggt ttgagtttct gttccgcctt ctaattttgc
     5761 ttctaattct ccggtagtca tagctttact tcaataagtt atcaaccgac acatcaaggg
     5821 ctttggctaa tttttggagc gtgtcaatcg agggattagt gataccgccc gactccagct
     5881 ttattaccgt gttataggaa atatcagcta atttagaaag ccggtcttgc gacaagcctt
     5941 tttgttttct taaccttttt atatttttac ctattggcga aatctctttt gacattatag
     6001 tattagaata atagtatgta tgtataagta atcacaatct ataattatga tacaaaatta
     6061 ttttgataat gtaaaaacat ttttagggag tttaattcca aatacccgcg cacgggaggg
     6121 ctggggcaag ggcatttacc ttttttcttt tctaaaaatc atcagctcgc ccgcgaagcg
     6181 ggcatggcgg gcgggcaaaa agaaaagggg gtctggggga aatgaatttt tgcccgcccg
     6241 cccgttttgg gtggtgggtg ggtgtgcgtc gccttcggcg acgcacaagt cagaattcag
     6301 cgtcggggtt ttgttcaaaa taagttcgga ttttgtccaa aaggtaccgc catttttcag
     6361 aattcggatt ttcagcgatt gttgcctcgc cctcgctacg ctcgggctcg gcaaaaacga
     6421 ggattttata gggatttttg aaatccaaaa acaatttctg cccgaacaaa cggaagttcg
     6481 gtttgccaaa ggcaaacata aatttgaaaa atttatcaac caatcttttt tctttcgccg
     6541 aaggcgaaag gcaaagcgat aaattcaaat attttgactg cccaatagta tgacaaacaa
     6601 aaataatcaa accaaaaaat ttttcatcta cgcccgcaag tcaacggaca cagaagaccg
     6661 acaagtaaga agcattcagg accaaatcgc ggaacttaaa gaattggcga aaaaggagaa
     6721 tctggaagtt gttgatattc ttgttgaaaa acaaaccgcc aagaagccgg gccgtccggt
     6781 ttttgccgaa atgctgaaac gaattgaagc cggcgaggca gagggaattt tagcgtggca
     6841 ccctgataga ttagcgagaa attccgttga cggcggacaa attatttatc tcgttgatac
     6901 cggaattatc aaagagctaa aattcccgac tttttggttt gaaaacacac cacaaggaaa
     6961 atttatgctc tctatcgctt ttgggcagtc aaaatattac attgataatc tttcggaaaa
     7021 tatcaaaaga ggacacagac aaaaattgaa aaatggttta tggccccaaa tggcgccact
     7081 cggttatctc aataacaagg aaacaaaaca aatctatgtg gataaagaaa aagccccgct
     7141 tattaaaaag acatttgaac tttatgcgac cggaaaatat acgctgaaag aaatccgaaa
     7201 gattatcaat gatttaggat taaaaggccg gcgaggtaaa atgctttccg tttcaaacta
     7261 tcaatacatt ttgcaaaatc ctttctacta cggacttatc cgctacaacg gcgaatacta
     7321 cgaaggaaag cacgaaccga ttattgcaaa gaaacttttt gacctctgtc aagaagtaat
     7381 gaaaagaaaa tctaaaccac agaaagcgga caagatgaaa ttttttcttt atcgcggatt
     7441 attcaaatgt ggagagtgtg gctacacaat taccgcagac agaaaaatta agaaaagtgg
     7501 aaaatcatac acttactact attgcaccaa gaaaaaccca aatcataaat gctcgcaaaa
     7561 tgtttttacc agagaagaaa aaatttcatc tcaaatcaaa gaggtaatac aaaaagtttc
     7621 tttgcctgat gattgggcgg agaaaatgct aaatgaattg gaaaaagaga aaaatgaaac
     7681 cgcccaatca tcacgttttt ttgctcaaaa aattcagtac gagataaaaa taattgacga
     7741 aaaactggaa aaactgatga atgcctatct tgaaaacgct ttgtctttgg acgaatacaa
     7801 agaagctaaa aacaaattag tcaatcaaaa acagcttttg aaagacaaac tgaacgcttt
     7861 tgagcaaaaa agcaataatc ggttcgaact cgccgccaaa ttcataaata ccgtaaaaca
     7921 agccgaaatt atcgctttgc aaaaaaatcc cgagcaaagc cgtgattttc taaaaaagat
     7981 tggttcgaac ttccgtttga gcgggcagaa attgtttttg gatttcaaaa atcccttcaa
     8041 aatccttgcc gaagccgagc cccgcctttg gcggggcgag gcaacaatcg cccaaaatcc
     8101 gaattctgaa aactggcggt gccttttggt taaaatccga accttttttg acgaaaatcc
     8161 ggactgcg
//
LOCUS       DAOSIB010000083         8133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_8225_length_8133_cov_10.346868, whole genome shotgun sequence.
ACCESSION   DAOSIB010000083 DAOSIB010000000
VERSION     DAOSIB010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8225_length_8133_cov_10.346868"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="PLD44_07350"
     CDS             <1..1299
                     /locus_tag="PLD44_07350"
                     /inference="COORDINATES: protein motif:HMM:NF037079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOF99182.1"
                     /translation="EDGGIIKAFNNYMTGQNRFVAYGSSDFANSTVDFDAYVVASRSE
                     TVPSTVKTKQGSNTYNNFDTDASVMYQYTADTPEQAKEKVMQYAGRVNGGDLKWTFNN
                     AVDDASYDVNTGLKAALTNYSTSLVAVQGESSSSGSSQTLTVTGDVNQTVTSGNAISN
                     IVFTWGGEATDASVNGLPASGIDYIKDAAAKTITISGTPTATVSYTVTTSGQSGTPVS
                     ISGTIIVTSGSGSSGAMIHNFTTDGKTSSFYSITGNLSTSYGTVTYQGLTLTQCLKME
                     SSTNISFTTTSEATLTLVFNPDYTGTVKIDGTTYTPSSTNGIVTISSLPAGSHTIVKG
                     SGSSYLFYMSTVYNSISGISNTNQSTSIQLYPNPVSNLLNINSNEKVTKIEIFSISGQ
                     IITQLNGNSSSIDLSNLSSGNYLIKIHTQNGVLTKLIVKK"
     gene            1392..3290
                     /locus_tag="PLD44_07355"
     CDS             1392..3290
                     /locus_tag="PLD44_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOF99183.1"
                     /translation="MKFSHSIFLFGAIVLCFPVKAQTVMINGVPRDTGYTVYNTYIKL
                     KKQFPFIRPAEARMSDNLKSYENIAYKSISPERKLLLNIYRLDNNEVLPAVIMVHGGG
                     WNSGSPSLQKAMAVKLAQKGFVCITVEYRLIPEALFPAGEEDLEDAVRWIADNAETYG
                     INRDKIAVSGCSAGGQLAALIGTKNKDKLIKTVVNIDGISSFIDKATIDRAQKARNIG
                     DKMPVDALWLGGTFAERPENWKAASAVTWVNQNSAPVCFINSSIPRFHNGRDEHIRML
                     DSLGIYSEVHAFDDCPHSFWHFHPWQLSTVQYVANFLNRILYNTPIAVNHSKYDLIVA
                     QDGTGDFRTVQKAINAVPDFRKRKTSIFIRNGFYREKLIIPETKDSLTLIGEDRNKTI
                     LSYNNFASKPSGFGDQLGTSGSASVYICSPNFTAENLTLENAAGPIGQAVAAVVRSDK
                     ARFLNCNFWGFQDTLYPHKAGSRQYYKNCYIEGMVDFIFGFSTAYFDSCELYCKESGF
                     ITAAATPQENNYGFVFYRCQIHGENPASFYLGRPWRPYAKVTFIECDMTNVIKPEGWD
                     NWGKVSNEKTAQFSEYQNSGEGGNLTNRRVKWSKTLSSRQVKNFDKEIVLGKDFFEKK
                     EDNHINKK"
     gene            3292..4503
                     /locus_tag="PLD44_07360"
     CDS             3292..4503
                     /locus_tag="PLD44_07360"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HOF99184.1"
                     /translation="MFKISRSISLIVFALLIHNVYSQSVTVKNQSNVLLKDFMVEIPV
                     NKLPLTIGNYYVVSKEGVQLPVEIVSDINAEQIVVFSIERLQPNAILKFNFQRGQAEN
                     YPKRTYAEIAHKIGGQFVNGKYQGEFSWVKPNFIRLPGNFKDHSYFIKYEGAGWESDK
                     VAYRFYLDQRNALDAFGKKTPGIVLPAVGVDGYDNYHKMAVWGMDDMEVGKSLGIGSI
                     AYWDGKKAFRVEKRDSLVCCVISDGKVRSQIKTVYYGWEAANKVNLTSLITIDAGHRA
                     SHMVLKIDGKLDNIATGIIKLKNTEIIVPPANDGEWTYYATFGCQSMNNDNMGLAIFY
                     RKRQLQTLTEDELNHVIVLTPDHGYVEYYFMATWEKEWQPIQDKASFINAIDDELNCL
                     NQKVSIRVHKK"
     gene            4664..6070
                     /gene="uxaC"
                     /locus_tag="PLD44_07365"
     CDS             4664..6070
                     /gene="uxaC"
                     /locus_tag="PLD44_07365"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOF99185.1"
                     /translation="MQSFINDDFLLQSDAAKELYHQHAEHQPIIDYHCHLNPEYIAND
                     RQFDNLGQIWLEGDHYKWRAMRSNGIDEKYCTGKDTSDWEKFEKWAETVPYTMRNPLY
                     HWTHLELKQAFGVDKLLSPATAKEIFDECTAKLRTKEYSARGLMKKFNVEVVCTTDDP
                     VDSLNYHIALKQEGFGIKVLPAWRPDKAMAVENPVEYRKYVEKLSEVSGVTIRKFDDL
                     LQALRVRHDFFADLGCKLSDHGIEEFYAEKWSKSEIEVIFNKVYGGKELTQEEIRKFK
                     SAMLYEGAVMDWEKGWTQQFHYGAIRNNNSRMMKQLGPDTGFDSIGDFTVAKAMSAFF
                     DSLDVSGKLTKTIIYNLNPRDNDLVATMIGNFQDGSVPGKMQFGAAWWFLDQKSGIEA
                     QLNSLSNLGLLSRFVGMLTDSRSFLSYPRHEYFRRILCNLIGNDVENGLLPASEMDFL
                     GKMVENISYFNAKNFFEF"
     gene            6098..>6600
                     /locus_tag="PLD44_07370"
     CDS             6098..>6600
                     /locus_tag="PLD44_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOF99186.1"
                     /translation="MKLGKYSFGVGDRFSHQGKAQLKALMKANEKGLNIIPVWNKSNR
                     EHTYVHSQPKDTRVEADNAVKALNYKGPYFVDADHINLNTVAPFVPVSDFFTLDVAAY
                     IGKESTKEEVNQFIDSCKKYMGQLEIPGLKEPLNVSKDLLEKITNKFLAATQQASEIY
                     AYLKKEKG"
     assembly_gap    6601..6700
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6701..7168
                     /locus_tag="PLD44_07375"
     CDS             <6701..7168
                     /locus_tag="PLD44_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOF99187.1"
                     /translation="DKFSIYPVMSRIIKKHDKGLHLKTAGTTWLEEVIGLAKAGGDGL
                     MAAKKIYANSYNRKDELCAPYADVIDIDGSKLPSVEEVNAWGSEKFVNTLRHDQTCPD
                     YNPNFRQLIHVAYKVAAEMGDEYISLLEKYEEIIGQCVEENIYDRHLKRLFDL"
     gene            7233..8045
                     /locus_tag="PLD44_07380"
     CDS             7233..8045
                     /locus_tag="PLD44_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOF99188.1"
                     /translation="MYELFDIKDKVIAFTGGTGVLGESMVKYLAAHGAKIAVLARSKE
                     KGDKLVEEVKGKGGEAMFLVTDVTNETILRQNVEDILAKYGKVDVLINAAGGNMPGAT
                     IGPNNTIFDLKLDDFRKVVDLNLMGSVIPTIVFAECMVKANKGNIINFSSASALRPLT
                     RVAGYGAAKAAVTNFTKYMAGELATKFGENFRVNAICPGFFITEQNRTLLINPDGSYT
                     DRGKAVIAHTPFRRFGVPEDLLGTMHYLVSDASKFVTGTVAIVDGGFDAFCI"
BASE COUNT         2530 a         1437 c         1712 g         2354 t
ORIGIN      
        1 gaagatggcg gtattatcaa agcatttaac aattacatga ccggtcaaaa tcgttttgtt
       61 gcttatggaa gctccgattt tgccaattca actgtcgatt tcgatgctta tgtagttgct
      121 tcaagaagtg aaactgttcc ttcgacagtg aagacaaaac aaggctcaaa tacctataat
      181 aatttcgata cggatgcgtc tgtcatgtac caatatacag cagatacccc agaacaagca
      241 aaagaaaaag taatgcaata cgccggacga gttaatggtg gcgatttgaa atggacattt
      301 aacaatgctg ttgatgatgc atcttatgat gtaaatacgg gtttgaaagc ggctcttaca
      361 aattatagta caagtttagt agccgttcag ggtgaaagta gttcttccgg cagctcgcaa
      421 acattgactg taacaggtga tgttaatcaa acggtaacat cgggaaatgc cattagtaat
      481 attgttttca cttggggtgg tgaggctaca gatgcttcag ttaacggatt gccggcttca
      541 ggaatcgact acataaaaga tgctgctgcc aaaacgatta ccatttccgg tacaccaaca
      601 gctactgttt cctatacggt aaccacaagc ggacaatcag gaactccggt ttcaatatca
      661 ggcacaatta tcgttacaag cggatcgggt tcttcgggag caatgattca taactttaca
      721 acggatggta aaaccagttc tttctattct attacaggca atctgtccac aagttacgga
      781 acggttactt atcaaggctt aaccttaaca cagtgcctca aaatggagtc gagtaccaat
      841 atcagcttta caactacttc ggaagctact ttaacgctgg tattcaatcc tgattatacc
      901 ggtaccgtta aaattgacgg aactacttac acgccgtcct ctacaaatgg aattgtaacc
      961 attagctcac ttcctgccgg atctcatacg attgtaaaag gaagcggctc cagttatctg
     1021 ttctatatga gtactgttta caattcaatt tcaggaatta gtaacacaaa tcagtcaact
     1081 tctatccagc tttatccaaa tcctgtttcc aatttattga acatcaattc aaatgaaaaa
     1141 gtaacgaaaa tcgaaatctt tagtatttca ggtcagataa ttacgcaatt aaatggaaac
     1201 agctcgagta ttgatttgag caatttgtca agtggcaatt atttaataaa aattcatacg
     1261 caaaatggag tcctaaccaa gctgatcgtg aagaaataat tcttattttg ggcaaatagc
     1321 agtgaagttt attcaagacc atattcattc actgctattt caccctttgt attgtatttt
     1381 aactttttat catgaaattt tctcattcaa tatttctttt tggtgcgatt gttttatgct
     1441 ttcccgtaaa ggcacaaaca gtaatgataa acggtgttcc gcgagataca ggctatacgg
     1501 tttacaatac ttatattaaa ctaaaaaaac agtttccttt tattcgcccg gcggaggcaa
     1561 gaatgtccga caatctgaaa tcgtacgaga acattgctta caaatccatt tcaccggaaa
     1621 ggaagctgtt gttgaatatt tatcggttgg ataacaatga agttttacct gccgtaatca
     1681 tggttcatgg cggaggttgg aattcaggtt ctccttcatt acaaaaagca atggcagtga
     1741 aactggctca aaaaggtttt gtctgtatta ccgtcgaata tcgtcttatt ccggaagctt
     1801 tatttcctgc cggagaagaa gatttggaag atgccgtaag atggattgcg gataacgcag
     1861 aaacttacgg aatcaatcgg gataaaattg ccgtttcggg atgctcggca gggggacaat
     1921 tggctgcttt aatcggaaca aaaaataaag ataagctcat caaaaccgtt gtcaatatcg
     1981 atggcatttc ttcctttatc gacaaagcga ccattgacag agcgcaaaaa gctcgaaaca
     2041 ttggagataa aatgccggtt gatgctttgt ggctgggcgg aacatttgcc gaacgtcccg
     2101 aaaattggaa agctgcttca gcggtaacct gggtcaatca gaattctgct ccagtctgtt
     2161 tcatcaacag ttccattcca cgttttcata atggcagaga cgaacatatc cgaatgttgg
     2221 acagtttggg catttattcc gaagtacatg ctttcgatga ttgtcctcat tctttttggc
     2281 attttcatcc gtggcaatta tctacggttc aatatgttgc caattttcta aatcgaattt
     2341 tatacaatac tccaattgca gtcaatcatt cgaaatatga tctgatcgtt gctcaggatg
     2401 gaacgggcga ttttcgaaca gttcagaaag ccatcaatgc cgtgcccgat tttcgcaaaa
     2461 ggaaaaccag tatttttatt cgcaacggat tttacagaga aaaactcatt attcctgaaa
     2521 cgaaggattc gttgaccttg attggagaag acaggaataa aacgattctg tcgtacaaca
     2581 attttgcatc aaaaccgagt ggttttggtg atcaactggg aacttccggt tctgcaagcg
     2641 tttatatttg ttcacccaat tttacggctg aaaatttaac tttagaaaac gcagccggac
     2701 cgattggtca ggcggttgca gctgttgttc gaagcgataa ggcacgtttt ttaaattgta
     2761 atttttgggg atttcaggac acactttacc cacacaaagc cggcagcagg caatactata
     2821 aaaattgtta catcgaaggg atggttgatt tcatttttgg cttttcaact gcttattttg
     2881 actcttgtga actttactgc aaagaaagcg gatttataac cgcagcagca acgcctcaag
     2941 aaaacaacta tggatttgtg ttttatcgtt gtcaaattca tggcgaaaat ccggcttcat
     3001 tttatttggg aaggccgtgg cgcccttatg caaaagtaac ttttatcgaa tgcgacatga
     3061 ccaacgtcat aaaaccggaa gggtgggata attggggaaa agtttccaat gaaaaaacgg
     3121 ctcaattttc ggaatatcaa aattcgggtg aaggtggtaa tttgaccaat cgtcgtgtga
     3181 aatggtcgaa aacgctttca tcccgacaag ttaaaaattt cgacaaggaa atagtactcg
     3241 ggaaagattt ttttgagaaa aaggaagata atcatataaa caagaagtaa aatgttcaaa
     3301 atcagtcgat caatatcttt aatagttttt gcattgctta ttcataatgt ttattcacaa
     3361 tcggttaccg ttaaaaatca atccaacgtc ttgttgaagg attttatggt cgaaattccg
     3421 gtgaataaat tgcccttaac cattggaaat tactatgtag tttcgaaaga aggcgttcaa
     3481 ttgccggttg aaattgtttc cgatataaat gctgaacaaa tcgtagtttt ttcgattgaa
     3541 agattgcagc cgaatgctat attaaaattt aactttcagc gtggacaagc tgaaaattat
     3601 cccaaacgaa cctatgcaga aattgctcat aaaattggag gtcagtttgt aaacggaaaa
     3661 tatcagggtg aattttcgtg ggtgaagccc aattttatcc gtttgcccgg aaattttaag
     3721 gatcattcct attttataaa atatgaagga gccggctggg aaagcgacaa ggtggcctat
     3781 cgattttacc tcgatcagcg aaatgcgctg gatgcctttg gaaaaaaaac gcccggaatt
     3841 gttttaccgg cagtgggcgt tgatggttac gacaattatc ataaaatggc cgtttgggga
     3901 atggacgata tggaagttgg caagtcgctg ggtattggca gcattgctta ttgggatggg
     3961 aaaaaagctt tcagagtcga aaaacgcgac agtttggtat gttgtgtcat ttcggatggg
     4021 aaagttcgtt cacaaataaa aaccgtttat tacggttggg aagcagcaaa caaagtcaat
     4081 ctaacttcac tgataaccat tgatgcaggg catcgggctt cgcacatggt attgaaaatt
     4141 gatgggaaat tggataatat agccacagga attataaagt tgaagaatac cgaaataatt
     4201 gtgcctccag ccaacgacgg ggaatggaca tattatgcta cttttggttg tcagagcatg
     4261 aataatgata atatgggact tgcgattttt tatagaaaaa ggcaattgca gactttgacc
     4321 gaagacgaac tgaatcacgt tatcgttctt acgccggatc atggttatgt tgaatattat
     4381 tttatggcta cgtgggaaaa ggaatggcag ccaattcagg ataaagcatc gtttataaat
     4441 gccatagatg atgagcttaa ctgtctgaat cagaaagtgt caatacgtgt gcataaaaaa
     4501 tgaaatggta tccattttaa agttattttc agctttgctt gttttatttg ttaatattat
     4561 gaaaaaaaat ttatatgcta attaaaatgg gaatattttt ataactttgt gtgcgcacac
     4621 agtttatatt tttacagaaa ataatctttt aaaacagaat attatgcaaa gttttattaa
     4681 cgatgatttt ttactgcaaa gtgatgcagc aaaagagctt tatcatcaac atgccgaaca
     4741 tcaaccgatt attgattatc attgccattt gaatcccgaa tacattgcca acgatcgtca
     4801 gttcgacaat ctgggacaaa tctggctcga aggtgatcat tacaaatggc gtgctatgcg
     4861 ctccaacgga attgacgaaa aatattgtac agggaaagac acttcagatt gggaaaaatt
     4921 tgaaaaatgg gctgaaactg ttccttatac catgcgcaat ccactgtatc actggacgca
     4981 tttggaattg aaacaggctt ttggcgtgga taaattgcta agcccggcta cggcaaaaga
     5041 aatttttgat gaatgtactg ctaagctccg tacaaaagaa tattctgcac gcggcttaat
     5101 gaaaaaattc aatgtggaag ttgtatgtac gaccgatgat ccggttgaca gcctgaatta
     5161 tcacatagct ttgaagcaag aaggcttcgg tatcaaagtg ttgccggctt ggcgtcccga
     5221 taaagcaatg gcggtggaaa atccggtgga atatcgaaaa tatgtcgaga aactttcgga
     5281 agtttcaggc gttaccattc gcaaatttga tgatttgtta caagcattgc gcgttcgtca
     5341 tgattttttt gccgatttgg gctgcaaact ttccgatcac ggaattgaag aattttatgc
     5401 agaaaagtgg tcaaaatcgg aaattgaagt cattttcaac aaagtttacg gtggtaagga
     5461 attaactcag gaagaaattc gtaaattcaa atcggccatg ctttacgaag gcgctgttat
     5521 ggattgggaa aaaggttgga cacagcaatt tcattacgga gctattcgca acaacaattc
     5581 caggatgatg aagcagttgg ggcccgatac cggattcgat tccattggcg attttacggt
     5641 tgcaaaagcc atgagtgcat ttttcgacag tttggatgtt tccggaaagc ttacaaaaac
     5701 tatcatttac aatctaaatc cacgcgacaa cgatcttgtt gccaccatga taggaaattt
     5761 ccaggatggt agcgttccgg gaaaaatgca attcggtgcg gcatggtggt ttctcgatca
     5821 gaaatccggc atcgaagctc aattgaactc actatccaat ttgggattgc tcagtcgttt
     5881 tgtcggaatg ttgaccgatt cacgaagctt tttgtcttat ccgcgacacg agtattttcg
     5941 tcgtattctt tgcaatttga taggcaatga tgtggaaaac ggtttgctgc ctgcttccga
     6001 aatggacttt ctgggaaaaa tggtggaaaa catttcttat ttcaatgcaa aaaacttttt
     6061 tgaattttga tttttattca tttaaaaaat attcataatg aaattaggaa aatatagttt
     6121 tggtgtcgga gacagatttt cgcaccaggg aaaagcgcaa ttgaaagctt taatgaaagc
     6181 caatgaaaaa ggattaaata ttattcccgt atggaataaa tcgaatcgtg agcatactta
     6241 tgtccattct caaccgaaag atacgagagt ggaagccgac aatgctgtta aagctttaaa
     6301 ttataaaggg ccatattttg ttgatgccga ccatattaat ttgaatacgg ttgctccttt
     6361 tgtaccggtt tccgatttct ttacactcga tgtggctgcg tatatcggaa aagaaagcac
     6421 aaaggaagag gtgaatcaat ttattgattc ctgcaaaaag tacatggggc aacttgaaat
     6481 tcccggatta aaggaaccat tgaatgtgtc aaaggatctg ttggaaaaaa taacaaataa
     6541 atttttggct gctacgcaac aagcttctga aatttatgcc tatctcaaaa aagaaaaggg
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gacaagtttt ccatttatcc
     6721 cgttatgagc cgaatcatta aaaaacacga caaaggctta caccttaaaa cagccggaac
     6781 aacatggttg gaggaagtta ttggattggc taaagccgga ggagatggac tgatggcggc
     6841 taaaaaaatc tacgccaact cttataaccg aaaagatgaa ttatgcgcac cttatgctga
     6901 tgtgattgat attgatggaa gtaaactgcc gtcagtggaa gaagttaacg catggggaag
     6961 tgaaaaattc gtaaacacgt tgcgtcatga ccaaacttgc cccgattata atcccaattt
     7021 ccgacagttg attcatgtag cttacaaagt tgctgccgaa atgggagatg aatatatttc
     7081 attgctcgaa aaatatgaag agattattgg ccagtgtgta gaggaaaata tttacgatcg
     7141 acatttgaaa agattgttcg atttgtgaaa actctctcag aaagaatgaa ttaattttat
     7201 aattttgaaa cataattcta aaaataaata tcatgtacga actttttgac attaaggata
     7261 aagttatcgc atttaccgga ggaacaggcg ttctgggtga atcgatggtg aaatatttgg
     7321 ctgcacacgg agcaaaaatc gctgttctgg ctcgaagcaa ggaaaaaggc gacaaactcg
     7381 tcgaagaagt gaagggaaag ggtggcgaag ctatgtttct ggtaactgat gttaccaatg
     7441 aaaccatttt aagacaaaat gtcgaagata tccttgcgaa gtacggaaaa gtggatgtgt
     7501 tgatcaatgc tgccggtggc aacatgcccg gtgctaccat tggaccgaac aacaccattt
     7561 tcgacctgaa actcgatgac tttcgtaaag tggtagattt gaatttgatg ggttctgtca
     7621 ttccgacaat tgtttttgcc gaatgcatgg tcaaagccaa caaaggaaat atcatcaact
     7681 tttcgtcagc ttctgcactt cgtccattga ctcgcgtagc cggatacgga gcagccaaag
     7741 cagccgtaac caactttaca aaatacatgg caggcgaact ggctaccaag tttggtgaaa
     7801 acttccgcgt caatgctatt tgtcccggat tttttattac ggagcaaaac cgcactttat
     7861 tgattaatcc tgatggttct tataccgatc gtggaaaagc cgttattgct catacacctt
     7921 tccgtcggtt tggcgttccg gaagatttgt taggaaccat gcattattta gtaagcgatg
     7981 cttctaaatt tgttacgggc actgttgcca ttgttgacgg cggatttgat gccttttgca
     8041 tttaattttc agtaccgaaa agtcttacag aacatttatt tgaaacccac atgtattgca
     8101 tacacaaaca cgaatgaata taatattcag aca
//
LOCUS       DAOSIB010000084         7889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_8519_length_7889_cov_11.206663, whole genome shotgun sequence.
ACCESSION   DAOSIB010000084 DAOSIB010000000
VERSION     DAOSIB010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8519_length_7889_cov_11.206663"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PLD44_07385"
     CDS             complement(<1..203)
                     /locus_tag="PLD44_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HOF99189.1"
                     /translation="MNKIVNIALQILPSSATIHPYKLVDKAIEVIAASGLKYRVTPFE
                     TVMEGTYDEVMEVVKKAQQACYDA"
     gene            complement(395..928)
                     /locus_tag="PLD44_07390"
     CDS             complement(395..928)
                     /locus_tag="PLD44_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOF99190.1"
                     /translation="MPVKIRLQRHGHKGYAFYHIVIADSRTPRDGRFIERIGSYNPNT
                     DPSSVDLKFDRALYWLQVGAQPTDTVRNILSREGVLMKKHLLEGVKKGAFDEAEAEHR
                     FEAWKSSKEAKLNKTKEQLTAEREAAEKARLSAEIEANKARAAELAKKKAAEEAKTKE
                     KDNSSENSEEQEPKEVE"
     gene            complement(1035..1985)
                     /locus_tag="PLD44_07395"
     CDS             complement(1035..1985)
                     /locus_tag="PLD44_07395"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99191.1"
                     /translation="MEKLSRYIEILLAQHDYVIVPGLGGFIIQPQSAMILSDRITPPL
                     AVIAFNPLLNSNDGLLAYEISEREHISYRAAIEMIDHEVESILSKLQQGEKVDMENLG
                     SLQKNTNGHLIFSPVPRADFLPANFGMTEIKLWKRRITDSEERKKITITLPSASSFRY
                     AAACALIFGMLLVSPRMADTKRSHYANLIPLETYTPTQSQPSASKNVSINNTLKATVE
                     TEKQDSLSPAKEENFQNALSKQTNLYHVIAATFYSRKSAEKFCAQLQNLKYTEAQVFS
                     PEKNYKVVVRSFPRYQEAVEFMEQLRKSDALFESAWVYCK"
     gene            2104..3168
                     /gene="rfbB"
                     /locus_tag="PLD44_07400"
     CDS             2104..3168
                     /gene="rfbB"
                     /locus_tag="PLD44_07400"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOF99192.1"
                     /translation="MEKTILITGGAGFIGSHVVRLFVNKYPDYHIVNLDALTYAGNLE
                     NLKDVADAPNYTFVKGDITDEEFIPQLFEKYDFDGVIHLAAESHVDRSISDPLAFIRT
                     NVFGTANLLNAAKEAWKGKMEGKRFYHISTDEVYGSLGKEGYFTEETPYDPRSPYSAS
                     KASADHFVRAYYHTYGLPVLISNCSNNYGSHHFPEKLIPLCINNIRYNRPLPVYGKGE
                     NVRDWLWVDDHAAAIDIIFHQGKIGETYNIGGHNEWTNIRLIEKLCEIMDRKLGRESG
                     ESAKLITFVKDRAGHDLRYAIDSSKLQRELGWQPSITFEEGLEKTVDWYLENQEWLDN
                     VINGDYQNYYEKQYSEREKS"
     gene            complement(3225..4346)
                     /locus_tag="PLD44_07405"
     CDS             complement(3225..4346)
                     /locus_tag="PLD44_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOF99193.1"
                     /translation="MKALKTKLKINYQSFGLKRIDTYIINKFLGTYFFCIVLILSISV
                     VFDLTEKLDNFFETNAPLKAIIFDYYLNFIPYYMNMFSPLFTFIAVIFFTSKMANNTE
                     IIAILSSGVSFRRLMLPYFISATLIALLFFFLGGYVIPNSSKKMLNFENQYVKKFKPS
                     NARNVQMEVEKGVIMYIERYEVDNNIGYHFSLEKFDGKKLVSRLTAETITWDSAYNWK
                     INNYLQRDFIGMREKITKGISMDTVIKVQPKELFITSEEAAQMTNSELRQYLHRQRAR
                     GVGNIQSFENEYYRRYAMPFAAFIMTLIGVSLSSRKVRGGMGLNLGIGLALSSIYILF
                     STLSTTFSVNGTLSPFMAVWLPNIVFLLIGIYLYQTAPK"
     gene            complement(4374..5504)
                     /gene="tgt"
                     /locus_tag="PLD44_07410"
     CDS             complement(4374..5504)
                     /gene="tgt"
                     /locus_tag="PLD44_07410"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOF99194.1"
                     /translation="MNFEILYTDSGSKARAGKITTDHGAIETPIFMPVGTAGSVKTVH
                     FRELEEDIHAQIILGNTYHLYLRPGTDILEQAGGLHKFIGWDHPILTDSGGFQVFSLT
                     GIRKLKEEGVLFQSHIDGSKHFFTPEITVDIQRTIGADIIMALDECTPGTADYQYAKK
                     SMQLTHRWLLRGIEHFDTVSPKYGYSQAFFPIVQGCVYPDLRRESAKFIADQNKDGNA
                     IGGLAVGEPTEKMYEMIEIVNDILPENKPRYLMGVGTPQNILESIERGVDMFDCVMPT
                     RNGRNGMLFTSQGVINIKNAKWKNDFSPLDEKGTSFVDKAYSKAYVRHLFAANELLGL
                     QIASIHNLAFYLWLMNEARLHIRKGDFSLWKKGMIEQLGRRL"
     gene            complement(5531..6121)
                     /gene="hisH"
                     /locus_tag="PLD44_07415"
     CDS             complement(5531..6121)
                     /gene="hisH"
                     /locus_tag="PLD44_07415"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOF99195.1"
                     /translation="MKVALVKYNAGNIRSVLFALERLGIEAVVTDDAEEILSADKVIF
                     PGVGEASTAMNYLKHNRLDLLIPSLKQPVLGICLGMQLMCSFSEENNTTCLGIFDEKV
                     KHFPANENFKVPQMGWNNIFRLSGPLFDGIKKNSFVYFVHSYYVPLSSNTAAVANYIF
                     DFSAALQKNNFFAVQFHPEKSGEIGQKMLENFLNIC"
     gene            complement(6137..6490)
                     /locus_tag="PLD44_07420"
     CDS             complement(6137..6490)
                     /locus_tag="PLD44_07420"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOF99196.1"
                     /translation="MQIEILKSKIHRVSVTDANLNYIGSITLDQDLMDAANIIPYEKV
                     SVVNNNNGERIETYVIPGERGSGMVCLNGAAARKVQIGDVVIIMAFATMDFEEAKKFT
                     PVVIYPDTETNQLKK"
     gene            complement(6538..7392)
                     /gene="panC"
                     /locus_tag="PLD44_07425"
     CDS             complement(6538..7392)
                     /gene="panC"
                     /locus_tag="PLD44_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOF99197.1"
                     /translation="MKVFSTKKELQKEMEQIRKEGKTVGFVPTMGALHEGHLSLINRS
                     VAENDVTAVSIFVNPTQFNNRSDLEKYPRDLQRDLLLLEKNGCDIVFSPSVDEMYDKN
                     EFESVFEFDFGGLDQVMEGKFRPGHFNGVVQVVSKLFNLVQPTKAYFGEKDFQQLAII
                     RRMVNLMNFDIEIVGCPIVREKDGLAMSSRNLRLNAEERQKAVEISKTLFESKKLISN
                     FSPEQVQNWVIARLNSFSGLEVEYFEIVHQTTLQPIHSWSEPAIGCIAVFCGEVRLID
                     NIKYNDVQ"
     gene            7704..7777
                     /locus_tag="PLD44_07430"
     tRNA            7704..7777
                     /locus_tag="PLD44_07430"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7738..7740,aa:Arg,seq:acg)
BASE COUNT         2307 a         1560 c         1518 g         2504 t
ORIGIN      
        1 gcatcgtaac aggcttgctg cgctttcttt actacttcca tcacttcgtc ataagttcct
       61 tccatcaccg tttcaaaagg agttaccctg tatttcagac ctgaagcagc aatcacttct
      121 attgccttat ccaccagttt atatggatga atggtagctg aactgggtaa aatttgaagt
      181 gcaatattga caattttatt catttttacg cagattaaat attgacaact acttataaat
      241 tgaaacgtat aaacagttta aaaattgtag ctttttgcaa tttatgttct aaaaataaca
      301 tcggaaaatt cttctctgtg ataaaacaaa aaagcccaaa aatacttccg ggcttttttt
      361 agagcaagtc aaatttgaat gtttttaaga ggatttattc tacttctttt ggttcttgtt
      421 cctcagagtt ttctgaagaa ttgtctttct cttttgtttt tgcttcttca gcggcttttt
      481 tctttgccag ttcggcagct ctggctttat ttgcttctat ttctgcagac agacgagctt
      541 tttcggcagc ttctctttca gcagtaagct gttctttggt tttgttcaat ttagcttctt
      601 ttgaagattt ccatgcttca aagcggtgtt cggcttctgc ttcgtcgaaa gcaccttttt
      661 ttacaccttc cagcaagtgt tttttcatta atacgccttc tcttgataaa atgttgcgta
      721 ccgtgtcggt gggttgtgca cctacctgaa gccaatacaa agctctgtca aattttaagt
      781 ctactgaact tggatcggtg ttgggattat aggaaccaat tctttcgata aatctgccat
      841 ctcgtggcgt acgactgtcg gcaataacga tatgataaaa agcatatcct ttgtgtccat
      901 gtcgttgtaa tctgattttt acgggcattt ttaaaatttt ttattgttaa tacctaattg
      961 ctttttctcg aaaagcggca caaagttatc atttttattt gataaccgaa atatttttat
     1021 cgataaaaaa tcaattattt acaatatacc catgcagatt caaacaaagc atcggattta
     1081 cggagctgtt ccataaattc cactgcttcc tgataacgag gaaatgaacg gacaaccact
     1141 ttgtaatttt tttccggtga aaatacctgc gcttcagtat atttcaaatt ttgaagttgg
     1201 gcacagaatt tttcagccga cttgcgggaa taaaatgtag ctgcaataac gtgatacaag
     1261 ttggtttgct tgctgagagc gttttgaaag ttttcttctt ttgcaggaga aagactatct
     1321 tgcttctccg tctcaacagt agcttttagg gtattattta tcgaaacatt ttttgaagct
     1381 gaaggctggg attgagtcgg tgtgtatgtt tccagcggaa tcaaattggc ataatgactg
     1441 cgcttggtat cggccatacg cggagaaacc agcaacatgc caaaaattag agcgcaagca
     1501 gcagcatatc gaaacgaaga agccgatggc aaagtaatgg taattttttt gcgttcttct
     1561 gaatcggtaa tacgtcgttt ccacagcttg atttcagtca tgccaaaatt ggcagggaga
     1621 aaatcggcgc gcggcacagg tgaaaaaatc aaatgtccgt ttgtgttttt ttgcaggctg
     1681 ccgagatttt ccatgtcaac cttttctcct tgctgaagtt tcgataaaat tgattctact
     1741 tcgtggtcta tcatttcgat ggcggctcga tagctgatat gttctctctc cgaaatttca
     1801 taagccagta ggccatcgtt ggaatttaac aacggattaa aggcaattac ggcaagcgga
     1861 ggcgttatcc tgtcggataa aatcatggcc gattgtggtt gtatgataaa tcctcccaat
     1921 cccggaacga taacgtaatc gtgctgagcc agcaaaattt ctatgtaccg acttaatttt
     1981 tccatgttgc aaatataata gaaagaattt ttaaaaaggt atttttacac aaaaaagttt
     2041 tcaacatttt gttaaatgtg aaaaatcgaa ttatctttgt atgattcaat tcagttatta
     2101 acgatggaaa aaactatact gattaccggt ggtgcaggtt ttattggttc tcacgtagtg
     2161 cgcctttttg taaataaata tcccgattac cacattgtaa atttagatgc gttaacttat
     2221 gcgggaaatc ttgaaaatct caaggatgtg gctgatgcgc ccaattatac ttttgtaaaa
     2281 ggagacatca cggatgaaga atttattcct caattatttg agaaatatga ttttgacggt
     2341 gttattcatt tggctgccga atcgcatgtc gaccggtcta tcagtgatcc gctggctttt
     2401 atccgaacta acgtatttgg cacagccaac cttttgaatg cagcgaaaga agcatggaag
     2461 ggcaaaatgg aaggaaaaag attttatcat atttctaccg atgaagttta tggttcgttg
     2521 ggaaaagaag gatattttac cgaagaaacg ccttatgatc cccgaagccc ttattcggcg
     2581 tccaaagcaa gtgccgacca ttttgtcagg gcttattacc acacgtatgg tttgccggta
     2641 ttgatttcca attgttccaa caattatggt tcgcatcatt ttcccgaaaa actgattccg
     2701 ctttgcatca ataatattcg ctacaatcgg ccacttcccg tttacggaaa gggagaaaat
     2761 gtgagagact ggttgtgggt tgatgaccac gctgcagcca tcgacatcat ttttcatcaa
     2821 gggaaaatag gcgaaactta caacataggc gggcacaacg aatggacaaa cattcgtttg
     2881 atagagaaat tgtgcgagat tatggacaga aaactgggac gtgaatccgg tgaatcagcc
     2941 aaactgataa cctttgtcaa agacagagca ggacacgatt tgcgttatgc catcgactcg
     3001 tcaaaattac agcgcgaatt aggctggcag ccatccatta ctttcgaaga agggctcgaa
     3061 aaaacagtgg attggtattt ggaaaatcag gaatggctcg acaatgttat taacggtgat
     3121 tatcaaaact attacgaaaa acaatattcc gaaagagaga aaagttgagt atggaataaa
     3181 ttgatttttt gcaatttcgc ctttcacaaa aatttcttct actcttattt tggtgctgtc
     3241 tgataaagat agatgccgat gagcaagaat acgatattgg gcagccatac agccatgaaa
     3301 ggtgaaagcg tcccattgac tgaaaaagtg gttgacagtg tggaaaacaa aatatatatc
     3361 gaactgagtg ccaatccgat tcccaaattg agtcccatac ctccgcgtac ttttcgcgac
     3421 gaaagcgaaa ctcctattaa agtcatgata aaagcagcaa acggcatggc ataccgtcta
     3481 taatattcat tttcaaaaga ttgaatattg cccacgccac gcgcacgctg gcggtgcaga
     3541 tattgtctga gttcagaatt ggtcatttga gcagcttctt ccgaagtaat aaataattct
     3601 ttgggttgaa ctttgataac ggtatccatt gaaattcctt tcgtaatttt ttcacgcatg
     3661 ccgataaaat cgcgttgcaa ataattgttg attttccagt tgtatgccga atcccatgta
     3721 attgtttctg cggtgaggcg cgaaaccagt tttttcccat caaatttttc cagtgaaaaa
     3781 tggtacccaa tgttgttgtc gacttcatat ctttcgatgt acatgattac acctttttca
     3841 acttccattt gcacgtttcg ggcattgctg ggcttaaatt ttttgacata ctgattctca
     3901 aaattcaaca tttttttgct ggaatttgga atgacatatc cacccagaaa gaaaaacaaa
     3961 agagcaatga gcgttgcaga tataaaatag ggaagcatta atcttctaaa actcacacca
     4021 ctggaaagaa tggcaataat ttcagtattg ttggccattt ttgaggtgaa aaaaattacg
     4081 gctatgaacg tgaacagggg gctgaacata ttcatgtaat atggaataaa attcaaataa
     4141 taatcaaaaa tgatggcttt gaggggcgca ttggtttcaa aaaaattatc caacttttca
     4201 gtcaggtcga aaacaacaga aatgctcaaa atcagtacga tacagaaaaa gtaagttccc
     4261 aggaacttgt tgataatgta cgtatcgatt cttttaagtc cgaaactttg ataatttatt
     4321 ttcaatttgg tttttaaagc tttcatgcca gaaaattttg aataaaggta ttttcaaagc
     4381 cttctaccga gttgttcaat cattcccttt ttccatagtg aaaaatctcc ctttcggata
     4441 tgcagtcggg cttcgttcat aagccaaaga taaaaagcca gattatgaat agaagcaatt
     4501 tgcagtccca ataattcgtt ggcagcaaac aaatgtcgca cataagcttt ggaataagct
     4561 ttatcgacga atgaagttcc cttttcatcc agcggcgaaa agtcattttt ccatttggcg
     4621 ttttttatgt tgataacgcc ttgcgaggta aaaagcattc catttcgacc attgcgcgtt
     4681 ggcatgacgc aatcgaacat atccactccg cgttcaatgc tttccagtat gttttgtggc
     4741 gttcccacgc ccatcaggta tcgaggtttg ttttcgggaa gaatatcgtt tacaatctca
     4801 atcatttcat acattttttc ggtaggttcg cccactgcca gtccgccgat ggcgtttccg
     4861 tctttattct ggtcggcaat aaattttgcc gattctcttc tcaaatcggg atagacacat
     4921 ccttgcacaa tagggaaaaa tgcttgggaa tatccgtatt tgggtgaaac cgtatcgaaa
     4981 tgttcaattc ctctgagcaa ccatcgatga gtaagttgca tcgatttttt tgcatattga
     5041 taatcggctg tgcccggtgt acactcatcc agagccataa tgatatctgc tccaatggtt
     5101 cgctgaatgt ccactgtaat ttcgggagtg aaaaaatgtt tgctcccatc gatgtgcgac
     5161 tgaaacaaaa cgccttcttc tttcaatttt cggataccgg taagcgaaaa aacttgaaag
     5221 cctccactgt cggtcaatat ggggtgatcc cagccaataa acttgtgcag tccacctgct
     5281 tgttccagaa tgtcagtgcc gggtcgcaaa tataaatgat aagtatttcc taaaatgatt
     5341 tgcgcatgaa tgtcttcttc cagctcgcga aaatgaacag tttttacgga acctgcggtt
     5401 cctaccggca taaaaatagg tgtttctatt gcgccgtgat cagtggttat tttccctgct
     5461 cttgccttgc tcccggaatc ggtataaagt atttcaaaat tcattcggtg tttctatcaa
     5521 aaaattccgt tcagcaaatg tttaaaaaat tttccagcat tttttgtcca atttcccccg
     5581 atttttccgg gtggaactga acggcaaaaa aattgttctt ttgtaaggct gcactgaagt
     5641 cgaaaatata attggcaaca gcagcggtat ttgacgacag tggtacgtag taactgtgga
     5701 caaaataaac aaacgaattt ttttttatgc catcaaacaa tggtccggat aaccgaaaaa
     5761 tattattcca gcccatttgt ggaactttaa aattttcgtt ggcaggaaaa tgttttactt
     5821 tttcatcaaa gatacccaga caagttgtat tgttttcttc agagaaggag cacatgagtt
     5881 gcatgcccag acagattccc aataccggtt gtttgagtga aggaataagc aggtcgagcc
     5941 ggttatgctt cagatagttc atggctgtag aagcttcacc tacaccggga aaaattacct
     6001 tgtcagcaga caggatttct tctgcgtcat cagttacaac cgcttcaatt cccagtcgtt
     6061 ccaaagcaaa aagtaccgat cggatgttcc ctgcattgta tttcaccaaa gcaactttca
     6121 ttgtctcaac cggtctttat tttttcaatt ggttggtctc cgtatcggga taaataacaa
     6181 ccggtgtaaa ctttttggct tcttcaaagt ccattgtagc gaaagccatg atgatgacca
     6241 cgtcgccaat ttgaactttg cgcgcagctg caccattcaa acaaaccata cctgagcctc
     6301 tttctccggg aataacgtaa gtctcaattc tttcaccatt attgttgttg accacactta
     6361 ctttttcgta tggaataatg ttggctgcat ccatcaaatc ctggtcgagc gtaatgctgc
     6421 cgatatagtt taaattggca tctgttaccg aaaccctgtg aattttcgat tttagaattt
     6481 ctatttgcat attttcaaat tctttgtgtt aaagaaataa agctgcagtt gtgcaactta
     6541 ttgaacgtca ttgtatttaa tattgtcaat cagtctcact tcaccgcaaa aaacagctat
     6601 gcagccaatg gcaggctctg accacgaatg aatgggttgc agtgtagttt gatgaacgat
     6661 ttcaaagtat tctacttcca agcccgaaaa ggaattcaat cgggcaatca cccaattttg
     6721 tacttgttca ggggaaaagt ttgaaatcag ttttttactt tcaaataaag ttttggaaat
     6781 ttcaacggct ttttgacgtt cttccgcatt gagccttaaa ttgcggctgc tcatggccaa
     6841 accatctttt tcacgcacaa tgggacaacc aacaatttcg atatcgaaat tcattaagtt
     6901 taccattcga cgaatgatgg ccaactgttg aaaatctttt tcaccaaaat aggctttggt
     6961 aggttgcacc aagttaaaca atttgctgac aacctgcaca acaccattga aatgtcccgg
     7021 cctgaatttt ccttccataa cctggtccaa tccgccaaaa tcaaactcaa aaacactttc
     7081 aaattcgttt ttgtcgtaca tttcgtcaac agagggagag aaaacaatat cgcaaccgtt
     7141 tttttcgagc aacagcaaat cccgttgcaa atcgcgaggg tatttttcca aatccgaccg
     7201 gttgttaaat tgagtaggat tgacaaaaat acttactgcg gtaacatcat tttcggctac
     7261 gctacgattg atcagggaca aatgaccctc gtgcaaagcg cccatggttg gaacaaatcc
     7321 gactgttttt ccttctttcc tgatttgctc catctccttt tgaagctcct ttttggttga
     7381 gaatactttc atgcgttttc accatttttt caacaagtcg aacagaactt ttgacgctaa
     7441 ctttaaagca gtcgaatcaa agattttcag ttccgaaaga cctaattttt tgaaaatttg
     7501 acagttccaa ttttgaatac catcaaaatt gggttacaaa attacgaaaa cttatttgga
     7561 tatgcacgat tgcttcttaa tttttcctaa agcaagccga agaccaattg tataaacttt
     7621 ggaacgttga atctactatc tacattttta aaaattttct ttgctcgttt taaaaatata
     7681 cattatcttt gcacttcgaa aatggccccg tggctcaact gaatagagca tctgactacg
     7741 gatcagaagg ttacaggttt gaatcctgtc ggggtcacat ttaatttaaa aaaggctgtc
     7801 ttgtcataaa agaaaagaca gcctttttgg atggataaaa aatcaaattc tgtcaattat
     7861 tcaattcttt gcactctcct tttccctct
//
LOCUS       DAOSIB010000085         7718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_8710_length_7718_cov_11.466527, whole genome shotgun sequence.
ACCESSION   DAOSIB010000085 DAOSIB010000000
VERSION     DAOSIB010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8710_length_7718_cov_11.466527"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1156)
                     /gene="pheS"
                     /locus_tag="PLD44_07435"
     CDS             complement(137..1156)
                     /gene="pheS"
                     /locus_tag="PLD44_07435"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOF99198.1"
                     /translation="MKDRILQLQEEISHLTAQTLEEVEALRIKYLSKKGEISQLFNDF
                     RNVSNEEKREIGQLLNELKNNAHDQINQLKEKLEANRGNVEKIDLTRTAEPIQIGTRH
                     PLSIVRNEIATIFSGIGFTIAEGPEIEDDWHVFEALNFPADHPARDMQDTFFIEKNPD
                     ILLRTHTSSVQTRVMTQQKPPIRMIFPGRVYRNEAISYRAHCFFHQVEGLYIDKNVSF
                     ADLKQALIYFAKEMFGMDTQIRLRPSYFPFTEPSAEMDISCNLCGGKGCPFCKYTGWV
                     EILGCGMVDPNVLENCGIDSKIYSGYAFGMGVERITNLKYQVKDLRMFSENDIRFLRQ
                     FESAY"
     gene            1368..2039
                     /locus_tag="PLD44_07440"
     CDS             1368..2039
                     /locus_tag="PLD44_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOF99199.1"
                     /translation="MTIQKILDFLSVLSKNNNREWFAQNKSWYDEVRSGYLDMSNSLI
                     MKIAEFDPEIKHVDIKDCIFRIYRDIRFSPDKSPYKTHFGAYIAAGGGRKSQRGGYYF
                     HLQPESSFVSVGIWSPEPNVLKALRKSVYENIDEFNDILSQPNFKRFFGGFFEDGKLK
                     KVPAGFPGDFPQADLLKLKHYATSSPLDGSILTDENPVPHIAEILEAGYPLNRFLNFT
                     LDELF"
     gene            2052..2498
                     /locus_tag="PLD44_07445"
     CDS             2052..2498
                     /locus_tag="PLD44_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOF99200.1"
                     /translation="MILDTLKNAELYESAHPLFKKAFDFLKTTDLKNLPLGKIELEGS
                     DLVVSVVDISGKTVQDARMEIHRKFIDIQLPLDGEEIMGWIPAEKLTKVLQPYDAEKD
                     IAFFADEAENLLKVRAMEFAVFFPSDGHQPGIGFGNHKKIIVKVRV"
     gene            2568..4568
                     /locus_tag="PLD44_07450"
     CDS             2568..4568
                     /locus_tag="PLD44_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOF99201.1"
                     /translation="MEILPIVKIDPWLEPYAGSIYGRYEYFLQTEKKLTGGKQSLSDF
                     ATGYLYFGLHRVADGWVFREWAPNATAIFLIGDFNNWQRHPDYQLKKLQNGIWEIHLP
                     EYAMGHTQLYKLLVEWEGGSGERIPAWAQRVVQDTQTKIFSAQVWNPFQPYEFKNRHF
                     KPQRDPLLIYECHIGMSSQEEKIATYDEFRRNVLPRIAALGYNAIQIMAIQEHPYYGS
                     FGYHVSSFFAPSSRFGTPEELKQLIDEAHGMGIAVIMDIVHSHAVRNEVEGLGRFDGT
                     PYQYFHGGPRREHPAWDSLCFDYGKPEVLHFLLSNCKYWLDVFHFDGFRFDGVTSMIY
                     LSHGLGEDFTSYDCYYNLNQDGDAICYLTLANKLVHQVNPNAISIAEEVSGMPGLAAK
                     IDDGGIGFDYRMAMNIPDFWIKIIKEEKDEDWKVGRIFWEMTNHRAEEMTISYAESHD
                     QALVGDKTIIFRLIDADMYWHMRRGDNTLTVDRGVALHKMIRLVTASTMNGGYLNFMG
                     NEFGHPEWIDFPREGNGWSYKYARRQWDLVDNKNYLYYCLNEFDRAMIMLIRSVPRFN
                     KLPIDQLWIKEDDQVLVFQRGKLVFVFNFNPSKSFTDYGILAEKGAYRTVLNSDNSLF
                     GGYDLIDESIVHVTLPEPTDPDGKEWLKLYLPARTAFVLKKD"
     gene            complement(4666..5061)
                     /locus_tag="PLD44_07455"
     CDS             complement(4666..5061)
                     /locus_tag="PLD44_07455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOF99202.1"
                     /translation="MATIFSKIAAGEIPSYKIAENDLYFAFLDIHPLTKGHTLVIPKK
                     EEDYIFRLDDESYQGLMLFAKKIALAIEKAIPCKRIGMAVIGLEVPHAHVHLIPIQQE
                     NDMNFRNPKLELSSEEMDKIAASIRKYLE"
     gene            complement(5088..5561)
                     /gene="greA"
                     /locus_tag="PLD44_07460"
     CDS             complement(5088..5561)
                     /gene="greA"
                     /locus_tag="PLD44_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOF99203.1"
                     /translation="MATVTYMTAEGYKKLVDELHQMENVQRPAISRQIAEARDKGDLS
                     ENAEYDAAKEAQGMLEMKIAILKDTIASARLIDESKIDTSEVQILTKVTIKNTKTNQV
                     MTYTIVSESEANIRAGKLSVTTPIAKGLLGKKVGEKTSISVPNGVQEFEILDISL"
     gene            complement(5707..6069)
                     /locus_tag="PLD44_07465"
     CDS             complement(5707..6069)
                     /locus_tag="PLD44_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99204.1"
                     /translation="MKKKKKSDTPKPEEKKHYEQATWQELKPVVERREFEKVVKAHTS
                     KPVEEYMENTYATYDTVGNDISELRIKKPVKVSIENNHHLIEEDRYEESSSIPLELDS
                     PEAAKKAFIYSVIFERKY"
     gene            6500..7615
                     /locus_tag="PLD44_07470"
     CDS             6500..7615
                     /locus_tag="PLD44_07470"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOF99205.1"
                     /translation="MKYGFFVVVFSIFAFFFGYVGFRGWQSLALYPSARPTYIVSFLL
                     LFICSMAGMIFGDDMPASIAKVLTFTGYSFLLIFAYLFIVFLLVDVVRIANHFFHFAQ
                     SNMVSFRFWSSIVGLSVIIIAMIIGNYKFNHPEVVQLELQSNKPFQNKKIRIVAASDI
                     HLGTYIDKNYLKKYVQFINDQRPNIILLAGDICDRSLKPLIHQKMYEELHQLKAPLGV
                     YAILGNHEYYSQNLQNTVLFYKDAGIVVLADSISFVNNDFYLVGRKDRTDKNRQPLST
                     LLTTLEQDKPIILLDHQPLHLVEAAKNNVDLQISGHTHEGQVFPANLLVKSLFENGYG
                     YLKKGNTHIYVSSGLGLWGPQYRIGTKSEIVVIDFKF"
BASE COUNT         2243 a         1396 c         1542 g         2537 t
ORIGIN      
        1 gtcacccgca acacccttgc cattcgctaa tgtttcctat caactcggca catacaggaa
       61 cccgcatcct gcaagcttaa tgccatgtca gcaatattct taaaagcgct ctctgttttt
      121 tctgaatact atcaaatcag tatgccgatt caaattgtcg caggaaacgg atgtcatttt
      181 cagagaacat gcgcaaatcc ttaacctgat attttaaatt ggtaattcgt tcaactccca
      241 ttccaaaggc ataaccggaa taaatttttg aatcgatacc gcaattttcg agtacattgg
      301 gatctaccat tccgcaaccc aatatttcta cccagcctgt atatttgcaa aacggacaac
      361 cttttccgcc acaaagattg cacgaaatat ccatttcggc gcttggttcg gtaaaaggga
      421 agtaggaagg acgcagccgg atttgtgtat ccatgccaaa catttctttt gcaaaataaa
      481 tgagtgcctg tttcaaatcg gcaaacgaca catttttatc gatgtataaa ccttctacct
      541 gatgaaagaa acaatgtgcg cgatacgaga tagcttcatt tctgtaaaca cgtcccggaa
      601 aaatcattct gatgggcggt ttttgctgtg tcatcacacg ggtttgaacc gaagaagtat
      661 gagtgcgcaa gagaatatct ggattttttt cgataaaaaa tgtatcctgc atgtctctgg
      721 cgggatgatc ggcaggaaag ttcaaggctt caaaaacatg ccagtcgtct tcaatttccg
      781 gtccttcggc aatggtgaac ccgatacctg aaaaaatcgt cgcaatttcg tttctgacaa
      841 tggaaagagg atgacgtgtt cctatctgaa tcggttcagc ggtgcgcgtc aaatcaatct
      901 tttccacatt tccccgattg gcttccaatt tttccttgag ctggttgatt tgatcgtgtg
      961 cattgttttt cagttcgttg agcaattgcc cgatttcccg tttctcttca ttacttacat
     1021 ttctgaaatc gttgaaaagc tgtgaaattt ctcccttttt gctcagatac ttgattctta
     1081 aagcttcaac ttcttcgagc gtttgggctg taagatggct gatttcttcc tgcaattgta
     1141 gaattcggtc tttcattttt tatattggaa tttgcaaaaa aattgaataa gtcctgagaa
     1201 cagaaataac ctgttattat ttttttaaat taactgcaaa agtaattatt ttttaccaaa
     1261 ataggaggaa tttataccgg aaaaaggaat tacttcaaaa aaatgaggat aaatggcaaa
     1321 gattaaataa attttacatt atatttgtcg tgcttaaaat ataaattatg accatacaaa
     1381 aaatactcga ttttctgagt gttttgagca aaaacaataa ccgtgagtgg tttgctcaaa
     1441 ataaaagttg gtatgatgag gtgcgtagcg gttatttgga tatgagtaat tcactgataa
     1501 tgaaaatagc cgaatttgat cccgaaatca agcatgttga cataaaagat tgcattttcc
     1561 gaatttacag ggatatacgt ttttctcccg ataaatcgcc atacaaaact cattttggtg
     1621 cttacattgc tgcaggcggt ggtagaaaaa gtcagcgggg tggttattat ttccatttac
     1681 aaccggaaag ttcttttgtt tcagtgggaa tttggagccc tgagcccaat gtgctgaaag
     1741 cgttacgaaa aagtgtgtac gaaaatatcg acgaatttaa cgatattctg tctcaaccga
     1801 atttcaaacg tttttttggc ggttttttcg aagatggaaa attaaaaaaa gtaccggcag
     1861 gttttcctgg tgattttccg caggccgact tgctcaaatt aaagcattat gcaacttcct
     1921 caccattgga cggatccatt ttaacggatg aaaatcctgt tccgcatatt gcagagattt
     1981 tggaagccgg ctatccgctc aacaggtttt taaattttac tttggacgaa ttattttaaa
     2041 cacataaagc catgatactc gataccttaa aaaatgccga attatacgaa tcggcacatc
     2101 cacttttcaa aaaagctttc gattttctga aaacaaccga cttgaaaaat ttgcctctcg
     2161 gaaaaattga gttggagggc agtgatttgg ttgtcagcgt ggtggatatt tccgggaaaa
     2221 cggttcagga tgcccgcatg gaaatacatc ggaagtttat cgacattcag cttccacttg
     2281 atggtgaaga aataatgggc tggattcctg ccgaaaagtt gacaaaagtt ttacaacctt
     2341 acgatgctga aaaagatatt gctttttttg ccgatgaggc agaaaatctc cttaaagtga
     2401 gagcgatgga atttgctgtt tttttccctt ccgatggtca tcaaccggga attggctttg
     2461 gaaaccacaa aaaaattata gttaaagttc gtgtttagga gtacaatttt atttccaacc
     2521 agaaaattta ttttgttttg cacaaaacca cattaagcta aaaaattatg gaaattcttc
     2581 caattgttaa aattgatccg tggctcgaac cttatgcggg ttctatttat ggaagatatg
     2641 aatattttct tcagactgaa aaaaaactga cgggaggcaa acagagtttg tccgattttg
     2701 caacaggata tttatatttt ggacttcata gagtggcgga tggttgggtt tttcgagaat
     2761 gggcacccaa tgctacggcc atttttttaa ttggcgattt caataattgg caacgtcatc
     2821 ccgactatca gttaaagaag ttgcaaaacg gaatttggga aatacatttg cctgaatacg
     2881 caatgggcca tacacaactt tacaaacttt tggtagaatg ggagggcgga agtggcgaaa
     2941 gaattccggc ttgggctcag cgtgtggtac aggatacgca aaccaaaatt ttcagtgcgc
     3001 aggtgtggaa tcctttccaa ccctatgagt tcaaaaatcg acatttcaaa ccacaaagag
     3061 acccgctgtt gatttacgaa tgccatatcg gcatgtcgag ccaggaagaa aaaattgcta
     3121 cttatgacga atttcgccgg aatgtacttc ctcgcattgc tgcgctgggt tacaatgcca
     3181 ttcaaattat ggctattcag gagcatcctt attacggttc gttcggctat catgtttcca
     3241 gcttttttgc tccttcatcg cgttttggta cgcctgaaga actcaaacaa ctgattgatg
     3301 aagctcatgg aatgggaatt gccgttatca tggatattgt tcattctcat gcagtgagaa
     3361 atgaagtgga aggattgggc agatttgacg gaacgcctta tcagtatttt cacgggggcc
     3421 cgcgacgtga acatcctgct tgggattctt tgtgtttcga ctacggcaaa cccgaagttt
     3481 tacattttct tttgtccaat tgcaaatact ggctggatgt ttttcatttc gacggtttcc
     3541 gtttcgatgg cgtaacatcg atgatttatc tcagtcatgg gcttggtgaa gattttacca
     3601 gttacgattg ttattataat ctgaatcagg atggcgatgc catttgttat cttacactgg
     3661 ccaacaaact ggttcatcaa gtaaatccca atgccatctc cattgcggaa gaagtgagtg
     3721 gaatgccggg attggctgca aaaattgatg atggcggaat tggcttcgat taccgaatgg
     3781 ccatgaatat tcccgatttc tggattaaaa ttatcaagga agaaaaagat gaagactgga
     3841 aagttggacg gattttttgg gaaatgacca atcatcgtgc cgaggaaatg accatcagtt
     3901 atgccgaatc gcacgatcag gctttggttg gcgacaaaac gattattttc agattgatag
     3961 atgccgatat gtattggcac atgcgacgtg gcgacaacac tttgactgtc gatcgcggag
     4021 tggcgctgca caaaatgatt cgtctggtta cggcttcaac catgaatggt ggttatctga
     4081 attttatggg gaacgagttc ggacatcctg aatggatcga ttttccgcgc gaaggcaacg
     4141 gttggtcata caagtatgcc cggcgtcagt gggatttggt ggacaacaag aattatctct
     4201 attattgttt gaacgaattt gacagagcca tgattatgct gattcgttcc gtgccaagat
     4261 ttaataaact tcccattgac cagttatgga taaaagaaga cgatcaggtg ctggtttttc
     4321 aaaggggaaa attggtattc gtatttaatt ttaatccttc aaaatcgttt accgactacg
     4381 gtattttggc ggaaaaaggt gcttacagaa ccgttttaaa ttccgacaat tccttatttg
     4441 gaggttatga tctgattgat gaaagtattg tgcatgttac cttgcccgag ccaacagatc
     4501 ccgacggaaa agaatggttg aaattatatc tccccgcccg aacagctttt gttctgaaaa
     4561 aagattgaaa agttttttga tttggagcta ctccatttga atacgacacc ccaaaagctt
     4621 cgagtaactt ttggggtgtt gcatgtaaag ttggaacaag gaaactcact ccaaatattt
     4681 tcggatagaa gctgcaattt tgtccatttc ttcggatgac aattccaatt tcgggtttct
     4741 gaaattcatg tcgttctcct gttgaatggg aataagatga acgtgagcat gaggcacttc
     4801 cagtccaatt actgccattc ctatccgttt gcagggaatg gctttttcga tggcaagggc
     4861 aatttttttt gcaaaaagca tcaatccctg atagctttca tcgtccaagc gaaaaatgta
     4921 atcttcttct tttttgggaa tcaccaaagt atgtcctttt gtcaaaggat gaatgtcgag
     4981 gaaagcaaaa taaaggtcgt tttccgcaat tttataactg ggaatttctc cggctgcaat
     5041 tttactgaaa atggttgcca tgattttaaa agtttaactt tccgttatta tagtgaaata
     5101 tcgagaattt caaattcctg tactccattg ggtactgaaa tggaagtttt ttcgcccact
     5161 tttttaccca gcaaaccttt ggctatcgga gttgtaaccg ataatttgcc tgctctgata
     5221 ttggcctcac tctctgaaac gatggtgtag gtcattacct gattggtttt ggtatttttt
     5281 atggttactt tggttaaaat ctgcacttca ctggtatcga tttttgattc gtcaatcaaa
     5341 cgtgccgatg caatggtgtc cttgagaatg gcaattttca tttcgagcat tccctgagct
     5401 tcttttgctg catcgtattc ggcattttcc gacaaatcgc ctttgtcgcg agcttcagct
     5461 atttgcctcg agatggccgg acgctggacg ttttccattt gatgaagttc atccaccagt
     5521 ttcttataac cttcagcagt catgtatgtt actgttgcca tagttttatt tttttaattt
     5581 gaataaaaat ataaaaacaa aaagaattcc gaatcatgta aagatcggaa ctctctctgt
     5641 tatgtttttc gatgtttatt tgactacaaa tatagtattt tttatgataa caaaaaaatt
     5701 ttgattttaa tattttcttt caaaaattac cgagtaaata aaagcttttt ttgcagcttc
     5761 cggtgaatcc aattccaaag gaattgagct ggattcttcg tagcgatcct cctctatgag
     5821 atgatggtta ttttcaatgg aaactttgac gggttttttg attcggagtt ctgaaatatc
     5881 gttgccgacg gtatcgtatg ttgcataagt attttccatg tactcttcaa ccggcttgct
     5941 ggtatgcgct tttaccacct tttcaaactc ccttctttca actacgggct taagctcttg
     6001 ccaagtcgct tgctcgtaat gttttttttc ttccggtttc ggtgtatcgc tttttttctt
     6061 cttcttcata ttgctcagta gcaccacgac tacgaaaata agaaatatgt aattgccaaa
     6121 gtcttccata atttattgtt gatgttattg aaaagcaaat ttacagtaaa tctttgaata
     6181 ttttaaaaaa aatagagaaa aaatcgattt atttaaaaca tcaatttcat cagaatataa
     6241 tttatttttt taaaggtctg atggagcgca catgtctgaa tattatattc attcgtgttt
     6301 gtgtatgtaa tacctgtggg tttcataatt aattatcaaa aaactatttt ctcggtatag
     6361 ggtcatgaac aattttcgta aaaatacgtt tttatgagca attgataatt caaaatttaa
     6421 agtgtcctat ttaatttttt ttgaaccaaa gccgctaaaa gtaaacgcac agctttgatt
     6481 aattaaacca aaatatatta tgaagtacgg attttttgtt gttgtttttt ctatttttgc
     6541 gttttttttt ggatatgtcg gttttcgtgg ttggcaatcg ctggccttgt atccatcagc
     6601 acgacctacc tatatagtca gttttctttt gttgtttatt tgttcaatgg ctggcatgat
     6661 tttcggcgat gacatgccgg catcaattgc caaagtttta acatttactg gatattcgtt
     6721 tcttctgatt tttgcttatt tattcattgt ttttttgttg gttgatgtgg ttcgaatagc
     6781 caatcatttt tttcattttg ctcaaagcaa tatggtttct ttcagattct ggagctcaat
     6841 tgttggttta tcggtaatta taatagccat gattataggg aattacaagt ttaaccatcc
     6901 tgaagtagtt caactcgaat tgcaaagtaa taaacctttt caaaataaaa aaatcagaat
     6961 tgtggctgcc agtgatatac atttgggcac ttatatcgat aaaaattatc taaaaaaata
     7021 tgttcaattc atcaatgatc agcgaccgaa tataattttg ttggcaggcg atatttgtga
     7081 tcgctccctc aaacctttga ttcatcaaaa aatgtacgaa gaattgcacc aactgaaagc
     7141 tcctttaggt gtctatgcca ttctgggcaa ccacgagtat tattctcaga atttgcaaaa
     7201 tacggtttta ttttataaag atgccggaat tgtagtactc gccgatagca tttcatttgt
     7261 caacaatgat ttttatcttg taggacgaaa ggacagaacc gataaaaaca ggcaacccct
     7321 ttcaacattg ttgacaacgc ttgaacaaga taagccgata atcttacttg atcatcagcc
     7381 gttgcattta gtagaagcag caaaaaacaa tgttgatttg caaatttcgg gacatactca
     7441 cgaaggacag gttttccctg ccaatttgct tgtaaaatct ttatttgaaa acggctatgg
     7501 atatttgaaa aaaggaaaca ctcatattta tgtttcgtct ggacttggtt tatgggggcc
     7561 gcaatatcga attgggacaa aatcggaaat tgtagtgata gattttaaat tctgaaagat
     7621 aagtctatag gccatatttt ttatgttttg caaaaagaaa aagtattttc caaatgtcaa
     7681 taaattcggt aaaagattga ataacattga tggataat
//
LOCUS       DAOSIB010000086         7671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_8788_length_7671_cov_12.031644, whole genome shotgun sequence.
ACCESSION   DAOSIB010000086 DAOSIB010000000
VERSION     DAOSIB010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7671
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8788_length_7671_cov_12.031644"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1277
                     /locus_tag="PLD44_07475"
     CDS             198..1277
                     /locus_tag="PLD44_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOF99206.1"
                     /translation="MGQNAPQYRLEKLPPPKEKVETIEILKQTTRSASALAELKGIAK
                     TIPNQAMLMNAIVLREAKDSSEIENIITTQDELYKALSINKTNISPATKEVVNYREAI
                     FFGFNLVKKQEFLRMSDIVSIQQQLVDNSAGIRSIPGTVLKNDKTGEVIYTPPQDKAE
                     ILDLLSNFVDYFNQPNDLPPLVNMAILHYQFESIHPFYDGNGRTGRILNILYLILNQL
                     IDVPILYLSSYIIANKTEYYRLLNQVNRMGEWEDWILFMLQAVENTSRETITRIMKIK
                     NLLDSTIDLVRQKANKVYTKELVELLFEQPYTKIEFVVKKLGVERKAASRYLKELENI
                     GVVESQKVGRETLYINKELMDILKQ"
     gene            1531..2733
                     /locus_tag="PLD44_07480"
     CDS             1531..2733
                     /locus_tag="PLD44_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HOF99207.1"
                     /translation="MGKVLIIGAGGVGTVVTHKVAQNPEVFTDIMLASRTKSKCDAVA
                     TEVKNRYGVEIKTASIDAENVPELVKLLKEFKPELVINVALPYQDLTIMDACLEAGVN
                     YLDTANYEPKDEAHFEYSWQWAYKDRFEKAGLTAILGCGFDPGVTSVFTAYAAKHHFD
                     EIQYLDIVDCNAGDHGKPFATNFNPEINIREVTQRGKYWENGQWVETEPHEIHKPLTY
                     PEIGPKESYLIYHEELESLVKNFPTIKRARFWMTFGEEYLTHLRVIQNIGMARIDEVE
                     FNGQKIVPIQFLKAVLPNPGDLGENYKGWTSIGCRIRGIKDGKEKTYYIYNNCSHEAA
                     YQETGTQGVSYTTGVPAMIGAMMFLQGKWKKPGVFNVEEFDPDPFMEQLNKQGLPWHE
                     LVNVDLEL"
     gene            complement(2799..2999)
                     /locus_tag="PLD44_07485"
     CDS             complement(2799..2999)
                     /locus_tag="PLD44_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99208.1"
                     /translation="MYLIIDMPMQKVTTSDNSADEVDSGQQKKTKVMCPQMETIERIL
                     QFAAAYRAEKFNEGQYVELLLN"
     gene            complement(3090..3683)
                     /locus_tag="PLD44_07490"
     CDS             complement(3090..3683)
                     /locus_tag="PLD44_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOF99209.1"
                     /translation="MKDFNQFTDDELVKLYKEGNNQAFEILLKRYQSKVYTYIFLIVR
                     NRELTEDIFQDTFIKAIATIQQDRYVESGKFLAWINRIAHNLIIDYFRREKNENTFSV
                     EGINFDILNCTRFSEKSMEDVLSNEQVFVDVVHLVEKLPLSQQAVVKMRFFENLSFKE
                     IAEKTNVSINTALGRMRYALINLRKIASEKNISLEIK"
     gene            complement(3860..5044)
                     /locus_tag="PLD44_07495"
     CDS             complement(3860..5044)
                     /locus_tag="PLD44_07495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOF99210.1"
                     /translation="MHVVFLKPKKEESVRRFHPWVFSGAIQKIEGQPSEGDLVKVLDS
                     QGNFLGIGHFQQGSIMVRLLSFQEEPIDENFWKNRIAEAYQMRKALGLMIQNRNNCYR
                     LVHGEGDGLPGLIVDVYGNTAVMQAHSVGMHKIRNVLAKCVVECVPEIQYLYYKSEAT
                     LPFRNENAPEDGYLIGGDSEQFVVMENGLKFFVDWQHGQKTGFFLDQRENRVLLERYA
                     SDRIVLNMFCYTGGFSVYALRGNAKLVHSVDSSAKAIELTDKNVALNFSDNRHQSFAE
                     DAFKYLQLYEKRAPFYDLIILDPPAFAKHRDAVPNALKGYRKLNRKAFEIIRPGGIIF
                     TFSCSQAITREQFRQVVFSAAAESKRNVRILHQLSQGTDHPINIYHPEGEYLKGLVLW
                     VE"
     gene            complement(5096..5800)
                     /locus_tag="PLD44_07500"
     CDS             complement(5096..5800)
                     /locus_tag="PLD44_07500"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOF99211.1"
                     /translation="MYSPKISKEEINQLPVVSFPGEIVLVDKPEMVAPAIDELRKNLV
                     VGIDTETKPSFKSGTSNKVSLLQLSELERCFLFRLNLIDFPQELQDFLNDENIKKVGL
                     SLHDDFRLLNKRQNFHPANFVDIQNIAKSYGIMELSLQKIYAIIFGKKISKAQRLSDW
                     EKPQLTEKQLRYAATDAWASLQIYLQLMNEPQLTPKQIEKLVQENTPHKQPETSENAE
                     TTNSDLKPDAHSEKLS"
     gene            complement(5808..6449)
                     /locus_tag="PLD44_07505"
     CDS             complement(5808..6449)
                     /locus_tag="PLD44_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HOF99212.1"
                     /translation="MSIDELPDHIVYSANVIEFVTVVAETCLFLEHTSEMGKDEFVEK
                     SLKLLPLLYLKASVLDIPEPVYENLPERFVTEEDYQFVREQIEQLLGNDDVYLEVFHP
                     DMAISDTPVAAFISENLSDIYQEIKDFAANYQTADVDIMSDALSECLTAFGEHWGQKL
                     LNAMRALHALKYNPQDDTEWEEMHQPDEKQLTRDSFLDFQHTAENDDVSPFLK"
     gene            6677..6916
                     /locus_tag="PLD44_07510"
     CDS             6677..6916
                     /locus_tag="PLD44_07510"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOF99213.1"
                     /translation="MEDKIVTLKNYDTIVKAMFDQEILRANHIECFINNEQLVELYPM
                     FADIDEGLKIVVFEKDLEKAQKVLEEYHSSIEENV"
     gene            complement(6913..7431)
                     /gene="folK"
                     /locus_tag="PLD44_07515"
     CDS             complement(6913..7431)
                     /gene="folK"
                     /locus_tag="PLD44_07515"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOF99214.1"
                     /translation="MSLIFLGLGSNLGEKEKNLQDAVMMLKMEIGSLLSQSSFYISKP
                     FGFSSENDFLNAVVLMETHLSPCELLDKTREIEQAMGRTSKSCGQQYADRIIDIDILL
                     YDHLVIDQPTLKIPHPAIAERDFVFIPLLEIAPDLVDPLSGKKFSELVEAKKITSELK
                     NFVVGKKEEKFR"
     gene            complement(7471..>7671)
                     /locus_tag="PLD44_07520"
     CDS             complement(7471..>7671)
                     /locus_tag="PLD44_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99215.1"
                     /translation="FGFIKKEYGIWGPSLLIALSGLLCLFFPHELGVKILVWIGALIT
                     LFFNRDKLMEKKGKVTEEVSQQ"
BASE COUNT         2508 a         1475 c         1396 g         2292 t
ORIGIN      
        1 ctgcttcaac taagttaatt tttttgctta cttttttgtt tcaagacaaa aaagtgagtg
       61 gggtttgggg caaagccccg tttgtacaat tttttatcat gtactaagat aaaattgtta
      121 tactttttca caaaagtttt acttcaaatt ttgttatttg taccaaaatt tgtatttttg
      181 ggacaaataa taagaatatg ggacaaaatg caccacaata caggcttgaa aaacttccac
      241 ctccgaaaga aaaagtggaa actattgaaa tattgaagca aacaaccaga tcggcatctg
      301 cattagccga attaaaagga atagcaaaaa caattcccaa tcaagccatg ttgatgaatg
      361 ctattgtcct acgcgaagca aaagacagct ccgaaatcga aaatattatc actactcaag
      421 atgaactcta caaagcccta agtattaata aaaccaatat atcgcctgct acaaaagaag
      481 tcgtcaatta tcgcgaagca atattttttg gttttaacct tgtgaaaaaa caagagttct
      541 taagaatgag cgatattgta agtattcaac aacaattggt tgataactcg gctggaatta
      601 gaagtattcc gggcacggtt ttgaaaaatg ataaaacggg agaagtaatt tatactcctc
      661 cacaagacaa ggcggaaata ctggatttgc tatcaaattt tgtggattat tttaatcaac
      721 ccaatgattt acctccattg gttaacatgg caattttgca ctaccaattt gaaagtattc
      781 acccttttta tgatggcaat ggtagaacag gcagaattct taatattctg tatctgatac
      841 taaaccaatt aattgatgtg ccaatattat atttaagttc atatatcatt gccaataaaa
      901 cagaatatta cagacttcta aatcaagtaa atagaatggg ggaatgggaa gattggatat
      961 tatttatgct tcaagctgtt gaaaatacct ccagagaaac cattacaaga atcatgaaga
     1021 tcaaaaatct gctcgattcg acaatcgatc tggttcgaca aaaagccaac aaagtttata
     1081 caaaagaact tgttgaactg ttattcgagc aaccttatac aaagattgag tttgttgtaa
     1141 aaaaattggg agtcgaaaga aaagcagctt cacgttattt aaaggaatta gaaaatattg
     1201 gagtagttga atcacaaaaa gtggggcgag aaacacttta tatcaacaaa gaattgatgg
     1261 atatactaaa acaataacaa taaacccaaa ccatgtccat tcattcataa aataacttgt
     1321 aataatcgaa gatcgatact ttttattgat gacaaaatag tttatacaaa tcttgaaaaa
     1381 taagttgaat tctgaccatt tagtaaatca gtaaaaaatt cccaaatctc aactcaaaat
     1441 ttttcttctt tcttttttcg tttccttaaa aaaatgtact tttgcaggct aatattttta
     1501 acttattaat tttcaacaca aaaaagaaaa atggggaaag tattgattat cggcgccgga
     1561 ggagtgggaa cggtcgttac gcacaaggtt gcacagaacc cggaagtttt caccgacatt
     1621 atgctggcaa gccgtaccaa atcgaaatgt gatgccgtag caacagaggt aaaaaatcgt
     1681 tatggtgtag aaattaaaac ggcttcgatt gatgctgaaa atgttcctga attagtaaaa
     1741 ctgcttaaag aatttaaacc ggaacttgtt atcaacgtag cgcttcccta tcaggatttg
     1801 accattatgg atgcctgcct ggaagccgga gtaaattatc tggacacggc caattacgaa
     1861 ccgaaagacg aagcccattt tgaatatagc tggcaatggg catacaagga tcgtttcgag
     1921 aaagcaggat tgacagccat tctgggttgc ggattcgatc cgggcgttac gagcgttttt
     1981 acggcttatg ctgccaaaca tcattttgac gaaattcaat atctcgatat tgtggattgc
     2041 aatgccggcg atcacggcaa accttttgcc acaaatttta atcccgaaat caatatccgc
     2101 gaagttacgc aacgcggaaa atactgggaa aatggtcagt gggtggaaac ggaaccgcac
     2161 gaaattcata aaccgttgac ttatccggaa atcgggccga aagaatcgta tctgatttat
     2221 catgaagaac tggaatcgtt ggtaaaaaat tttccaacca ttaagcgagc acgtttctgg
     2281 atgactttcg gagaggaata tttaacacat ttgcgtgtca ttcaaaacat tggcatggct
     2341 cgtattgacg aagttgaatt caacggacaa aagattgtgc caattcaatt tttaaaagcc
     2401 gtgttgccta atccgggcga tttaggtgaa aactacaaag gttggacttc cattggttgc
     2461 cgcattcgcg ggataaaaga cggcaaggaa aaaacttact atatctacaa caattgcagc
     2521 cacgaagcag cttaccagga aaccggcaca cagggtgtaa gttataccac cggcgttcct
     2581 gccatgattg gagccatgat gtttttgcag ggaaaatgga aaaagcccgg cgttttcaat
     2641 gtggaagaat ttgatccgga tccttttatg gaacaactca acaagcaggg tttgccctgg
     2701 catgaattgg tgaatgtaga tttggaactg taagtttttc agaaaaaaag aaaaagatat
     2761 ttataccaaa aaagagcgct tgtgggcgct cttttttgtt aattcagcaa taattctaca
     2821 tactgtcctt cgttgaattt ttctgctctg taagcagctg caaactgtaa aattctttca
     2881 atcgtctcca tttgaggaca cattactttt gtctttttct gctgaccgga gtccacctca
     2941 tcagcactgt tgtcggaagt agtaactttt tgcataggca tgtctattat taaatacatt
     3001 tttatctctc aaataataaa cgatgacact tgccaaaaaa gtgtacggac acaaacaaaa
     3061 ataaaaatat ttttttcgct tacctgaatt tatttgattt cgagagagat atttttttct
     3121 gaagctattt ttcgcaagtt gatcagcgca taacgcattc tgcccaaagc cgtattaatg
     3181 ctgacatttg ttttttcggc tatttccttg aaactcaaat tttcgaaaaa acgcatttta
     3241 accacagcct gctgagaaag aggaagtttt tcgaccaaat gaaccacatc gacaaaaacc
     3301 tgttcattag acaacacatc ttccatactt ttttcggaaa aacgtgtgca gttcaaaata
     3361 tcaaagttaa ttccttctac cgaaaaggtg ttttcgttct tttcgcgacg aaaatagtcg
     3421 ataattaagt tatgggctat tcgattaatc catgccagga attttcccga ttcgacataa
     3481 cgatcctgtt gaatggtagc tatggccttg ataaaagtat cctgaaaaat atcttcggta
     3541 agttccctgt tgcgtacaat caaaaaaatg taggtatata ctttcgactg ataacgtttc
     3601 aataaaatct caaaagcctg attgttaccc tctttgtaca attttaccaa ttcatcatcg
     3661 gtaaattgat tgaagtcttt cattattgct aatttattgg ttaagggtaa gaatcggcaa
     3721 taggcattga agatttagag gttgactatt aaaaaatttt tagtacgcaa aaataataat
     3781 tgtattgata taaaaaaatt ttttacaaca aaatttttca aaaaacgtta caaattttga
     3841 gtcattctat tctatttccc tattctaccc acaaaaccaa gcctttcaga tattctcctt
     3901 ccggatgata aatattgatg ggatgatctg ttccctgcga aagttgatgc aaaatacgca
     3961 catttcgttt gctttcggca gctgcactga acactacctg acgaaactgt tcccgtgtaa
     4021 tggcttgtga acaggaaaaa gtaaaaatta ttcctccggg ccgaatgatt tcaaaagctt
     4081 ttctgttgag ctttctgtaa cctttcaatg cattcgggac ggcatccctg tgtttggcaa
     4141 aagccggagg atcaagaata attaaatcgt aaaaaggtgc acgtttttcg taaagttgca
     4201 aatatttaaa agcgtcttcg gcaaaagatt gatgacgatt atcggaaaaa ttgagcgcta
     4261 catttttatc ggtcaattct atagctttcg ccgaactatc gactgaatga accaatttgg
     4321 catttccacg caaagcataa acggaaaaac cgcctgtgta gcaaaacata tttaatacaa
     4381 ttcgatcgga agcataacgt tccagcaaaa cgcggttttc acgttgatcg agaaaaaaac
     4441 ccgttttttg gccatgttgc cagtcgacaa aaaatttcag tccgttttcc atgaccacaa
     4501 actgttcgga atctcctcca attaagtagc cgtcttcagg agcattttca ttacgaaaag
     4561 gcagggttgc ttccgatttg taataaagat attggatttc gggaacgcat tctaccacac
     4621 atttcgctaa cacattcctg attttgtgca tacccaccga gtgagcctgc atgacagccg
     4681 tattgccata tacatctaca atcaatcccg gcaaaccatc tccttctcca tgaacaagtc
     4741 ggtagcagtt gttccggttt tggatcatca atcccaacgc tttacgcatt tgataagctt
     4801 cagctattcg gtttttccag aagttttcgt caattggctc ttcctgaaac gaaagaagac
     4861 gaaccataat gctgccctgc tgaaaatggc ctattcctaa aaaattccct tgactgtcga
     4921 gtacttttac caaatcgcct tccgaaggct gaccttcaat tttttgaata gcaccggaaa
     4981 atacccacgg atgaaatcgg cggacacttt cttctttttt gggtttcaga aaaacaacat
     5041 gcatattaaa tgttggattt taaaaattga ataattatca ttcgacaaaa caaactcagg
     5101 aaagcttttc tgaatgagca tcgggcttaa gatccgaatt tgtggtttcg gcattttcgg
     5161 atgtttcggg ttgcttgtga ggcgtgtttt cctgcaccag tttctcaatt tgtttggggg
     5221 tcaattgtgg ctcattcatc agttgcagat aaatctgtaa acttgcccaa gcgtcggttg
     5281 cggcatagcg aagctgtttt tccgtcaatt gcggtttttc ccaatcgctg agacgttgtg
     5341 ctttggaaat ttttttcccg aaaataatgg cgtaaatttt ctgcaaactc aactccataa
     5401 taccgtaact ctttgctata ttttgaatat ctacaaaatt agccggatga aaattctgac
     5461 gcttgttcag caaacgaaaa tcatcgtgca atgacaagcc gactttcttg atgttttcat
     5521 catttaaaaa atcctgcaat tcctgtggaa aatcgattaa atttagacga aaaaggaaac
     5581 aacgttccaa ctcggaaagt tgaagcaggg aaactttatt ggaagtaccg cttttgaaag
     5641 aaggttttgt ttcggtgtcg atccccacaa cgagattttt tcgaagttcg tcgatagcgg
     5701 gcgctaccat ttcaggttta tccacaagaa caatttctcc cggaaaactg actacgggta
     5761 actgattgat ttcctctttc gaaatttttg gtgaatacat aaaaaattta tttcaaaaaa
     5821 ggacttacat cgtcgttctc ggcagtatgt tgaaaatcga gaaacgaatc gcgcgtaagt
     5881 tgtttttcat caggttgatg catttcttcc cattctgtat catcctgcgg gttgtatttc
     5941 aaagcatgta aggcacgcat ggcattcagc aacttttgac cccaatgttc gccaaaagcc
     6001 gtaagacatt cagatagagc atcgctcatt atatccacat cagcagtttg ataattggca
     6061 gcaaaatcct tgatttcctg ataaatatca ctcagatttt cagaaataaa agctgcaaca
     6121 ggtgtatcgc ttatggccat gtcgggatga aacacctcaa ggtacacatc atcgttgccc
     6181 aaaagttgtt cgatttgttc acgaacaaac tgataatctt cctccgtaac aaaacgttca
     6241 ggaagatttt cataaaccgg ttcgggaata tcgagcacgg aagccttcag ataaagcaac
     6301 ggcaatagtt tcagcgattt ttccacaaac tcatccttcc ccatttccga agtatgctcc
     6361 agaaaaaggc aggtttcggc aactactgtt acaaattcaa taacattggc actgtaaacg
     6421 atatgatcgg gcaattcgtc aatactcatg ataatttcaa taaaacttat tcattgtagc
     6481 aatgaaaatt tttaaggtac aaaattaaga aaattatttt acataaaccg gtgaaatctt
     6541 tcaggcccca tctcaaaaaa agatagtcaa ctttcgaaaa atttgataaa caatttttat
     6601 ctttaccttg ttctaaacca aaaggacata gcaaaagcat aatttatttt ctaccaatac
     6661 cccaaaacaa agcagtatgg aagacaaaat tgtaacacta aaaaattacg acacaatagt
     6721 gaaagccatg ttcgatcagg aaattttacg tgccaaccac atcgaatgct ttatcaacaa
     6781 tgagcagttg gtggagcttt atccaatgtt tgccgacatt gatgaaggtt tgaaaatagt
     6841 tgtttttgaa aaagatttgg aaaaagcaca gaaggtattg gaagaatacc atagctcaat
     6901 cgaagaaaat gtttaacgaa atttctcttc cttttttccg acaacgaaat tttttaattc
     6961 gcttgttatt ttttttgctt caaccaattc tgaaaacttt tttcctgaga gcggatcaac
     7021 gagatcggga gcaatttcca gcaagggaat gaatacaaag tcgcgctccg caattgcggg
     7081 atgcggaatt tttaaagtag gttggtcgat gacaagatga tcataaagca atatgtcaat
     7141 atcaataata cggtcagcat attgttgccc gcaacttttt gaagttctac ccatcgcctg
     7201 ctcaatttcc cgggtcttgt ccaacaattc gcagggagat aaatgggttt ccatcagcac
     7261 taccgcattc aaaaaatcgt tttcggaaga aaaaccaaat ggtttggaaa tataaaacga
     7321 agattgggac agtaaactcc caatctccat ttttaacatc atcacagcat cctgcaaatt
     7381 tttttctttt tctccaagat tgcttcccaa acccaaaaaa atcagactca tttttaaaaa
     7441 gattactttc tttgcatttc tcaccaatga ttattgctgc gaaacttcct ccgtaacttt
     7501 tccctttttc tccatcagct tgtcccgatt aaagaacaag gtaataagtg cgccaatcca
     7561 aaccagaatt ttgacaccca attcatgagg aaaaaacagg caaagtaatc cactcagtgc
     7621 tatcagaagg gaaggtcccc aaataccgta ttcttttttg ataaaaccaa a
//
LOCUS       DAOSIB010000087         7597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_8900_length_7597_cov_12.334261, whole genome shotgun sequence.
ACCESSION   DAOSIB010000087 DAOSIB010000000
VERSION     DAOSIB010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8900_length_7597_cov_12.334261"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..2115)
                     /locus_tag="PLD44_07525"
     CDS             complement(91..2115)
                     /locus_tag="PLD44_07525"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOF99216.1"
                     /translation="MNSIELLNKAADNIRILAAAMVEKAKSGHPGGAMGGADFMNVLF
                     SEFLEYDPENPTWESRDRFFLDPGHMSTMLYSVLAFSGKFTLDELAQFRQWGSPTPGH
                     PELDVMRGIENTSGPLGQGHTYAVGAAIAAKFLKARFGDVMNQTIYAYISDGGVQEEI
                     SQGAGRIAGHLGLDNLIMFYDSNNIQLSTETKAVTSENTAEKYKAWGWKVIEINGNDA
                     AQIRQALTEAKAEKERPTLIIGKTVMGKGIRKADGSSFENQTSTHGMPISECGASFEE
                     TIRHLGGDPEHPFVIFEETKALYEKRKNELKEIVARKKTAKEAWAKEHPDLAKKLESF
                     FSGKAPEVDWEAVVQQPNKSTRAASATVLGELAKQVENMITASADLSNSDKTDGFLKK
                     TKAFSKNDFSGSFLQAGVSELTMACLCIGMSLHGGVVPACGTFFVFSDYMKPAVRMAA
                     LMEQPVKFIWSHDAFRVGEDGPTHQPVEQEAQIRLMEKLKNHHGRNSMLVLRPADVEE
                     TTVAWKMALENIKTPTGLILSRQDIKDLPSTNGRKEEAKKAEKGAYVVQCDGNPDIIL
                     LASGSEVSTLNEAAVLLRNDGIRVRIVSVPSEGLFRLQSKEYQEMVLPQEVKKFGLTA
                     GLPVTLQGLINNNGKVFGMESFGFSAPYKVLDQKLGFTGENIYHQIKLML"
     gene            complement(2162..2866)
                     /locus_tag="PLD44_07530"
     CDS             complement(2162..2866)
                     /locus_tag="PLD44_07530"
                     /inference="COORDINATES: protein motif:HMM:NF039966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HOF99217.1"
                     /translation="MFQPSMQNIYRYFIISLFVAVAVNFSTAQNKTPASAKSPEVKWW
                     NGFLVRMDVASPVITGFSDTEHFGMEGSVQFNLKRKIFPVYEIGFGGADKLSSNEVRF
                     NTNGIFQRIGIDFGLLKQSNDKELINNMLTGGVRLGWSNFSYDLTNLSLSDDYWGGSQ
                     TLNFEGQSATKTWYEIIVGIQVQVYKNFYLGWNIRNRHFFKEDTSGNITPWYIPGYGK
                     TGSSNTGFNYTIGYHF"
     gene            complement(2820..3386)
                     /locus_tag="PLD44_07535"
     CDS             complement(2820..3386)
                     /locus_tag="PLD44_07535"
                     /inference="COORDINATES: protein motif:HMM:NF040109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HOF99218.1"
                     /translation="MIFINFKNLFNTLKNFLQNNFTKSTVFIWITAVLFFSTSCNVSD
                     EVCRKEKNSRLKVGFYTAVFNSSTGKYTSSSLAIDSLTVNGVDSLGNIVDSILYNNSK
                     NVSVIKLPLNKFSNESKFIIRFNDSIQDTLTVFHTNYDQYLSFECGMIRNHLIDTVLI
                     TNHFVDSVKIEEHNVSTIDAEHLQIFHN"
     gene            complement(3420..3938)
                     /locus_tag="PLD44_07540"
     CDS             complement(3420..3938)
                     /locus_tag="PLD44_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99219.1"
                     /translation="MAEILKLLIPALLVLLTAYLLLDRLLKNEEKRRNFELFKSNYSV
                     ITPIRLRAYERLMLVLERTIPNNLILSTIKPGMTNLELQSQLLNTIRHEFAHNLSQQI
                     YVSDELWNYISSAQESLLRLINMCASQCNPNEPASLLAEKILEIYAASEETPTEQAIR
                     KLKEEVRKYFPS"
     gene            complement(3967..4320)
                     /locus_tag="PLD44_07545"
     CDS             complement(3967..4320)
                     /locus_tag="PLD44_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HOF99220.1"
                     /translation="MKKNENSILKIYASSTDKMGNELLYEHLVFLAKENGIAGVTVYR
                     GIMGYGLSSQKISTSRFWELTEKLPVVVEMMDTTEKLESFYRTIEEKLKNIPNGCLVT
                     LQPVEVKLHKFGNKK"
     gene            complement(4322..4696)
                     /gene="crcB"
                     /locus_tag="PLD44_07550"
     CDS             complement(4322..4696)
                     /gene="crcB"
                     /locus_tag="PLD44_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOF99221.1"
                     /translation="MKQLLIVGLGGFFGTMARYLVYRINLLENFLSLPLGTLTVNVSG
                     SFLIGILTGLASKSELLSADMRLFLMAGFCGGFTTFSSFTNENLQLIQNGQFLSAAVY
                     TVISLLLGFLAVFLGYAVTNFL"
     gene            complement(4767..5720)
                     /locus_tag="PLD44_07555"
     CDS             complement(4767..5720)
                     /locus_tag="PLD44_07555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOF99222.1"
                     /translation="MDISVVVPLYNEEESIRPLYEWIERVMNENGFSYEVIFVNDGST
                     DRSWEIIESLKASSPEHVHGIKFRNNYGKSVALHFGFQAAKGDVVITMDADLQDSPDE
                     IPSLYDMIKNQNYDLVSGWKKKRYDAKLTKNFPSKIYNATARRVTKLQLHDMNCGLKA
                     YRNEVVKSIELYGEMHRYIPYLAKIAGFTKIGEKVVEHRKRQFGTTKFGWNRFVNGYL
                     DLMTLWFLSKFGKQPMHFFGLIGTLIFLLGLIAVIVVGAKKLIALSAHVSAPLVTSSP
                     YFYLAILAMILGTQLFLAGFVAELITRNSSERNNYLIEKEI"
     gene            complement(5845..6351)
                     /locus_tag="PLD44_07560"
     CDS             complement(5845..6351)
                     /locus_tag="PLD44_07560"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOF99223.1"
                     /translation="MQGNIFKSSMIYGLMMGILFSVNFIISIPQGAIFRIVSFFITIV
                     IVIVMYRYSVKYRDNECGGYITYGKSFSFIVLTFFFASIISAIVKFVYFQFINPEYLS
                     NVLQQSMKVVESMNLPLDDEYYTQMEKMMKPSVYTLQSIWVNVLLGALLALVMSAFVK
                     KDKDIFEE"
     gene            6699..7559
                     /locus_tag="PLD44_07565"
     CDS             6699..7559
                     /locus_tag="PLD44_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOF99224.1"
                     /translation="MDKTQKQILEAQRTELTEHLIYKKLADDCKDENNAKILYEIGQQ
                     ELEHAHYWEKKTGIKVKPDYARIRRTVFMARLLGLSFVLKQMEKREGTGSKLYDELSA
                     VYPETKLFSEQENAHEQQLLAMLDEERLQYVGSIVLGLNDALVELTGALAGFTLALGN
                     TRTISLAGLVTGISAALSMAASDYLSTKAEGDTRAAKSAAYTGVTYFLTVILLILPFL
                     LMPNKFGALALTLSIAVLIIFCFNYYISVAKNLSFKPRFIEMTTISLGVAAFSFFIGY
                     LLNQILGVNV"
BASE COUNT         2342 a         1545 c         1375 g         2335 t
ORIGIN      
        1 gattcctata agaataatag taacattgtc catgacaaaa acggttatag aaaaaacaag
       61 aagcggattt cgcttcttgt tttttcgctt ttaaagcatc aattttatct gatgataaat
      121 attttctccg gtaaatccaa gcttttgatc caaaacctta tatggagcag aaaagccgaa
      181 agattccatt ccgaatactt ttccattgtt gtttatcagc ccttgcaaag ttacgggcaa
      241 ccccgcagtg agtccgaatt tttttacttc ctgaggcaaa accatttcct gatactcttt
      301 cgactggaga cggaacaatc cttcggaagg aacggaaaca atccgcaccc gaattccatc
      361 attgcgaagt aaaacggcag cttcattcaa agtcgaaact tccgatccgg aagcaagcaa
      421 aatgatatcg ggatttccgt cgcactgaac aacataggca cctttttcag cttttttggc
      481 ttcttcttta cgaccatttg tggacggaag atctttaatg tcctgacgtg aaagtatcaa
      541 gccggtaggc gttttgatgt tttccagtgc cattttccaa gctactgttg tttcttcaac
      601 gtcagccgga cgaagaacca gcatggaatt tcgtccatga tgatttttaa gtttttccat
      661 taagcgtatt tgtgcttcct gctccaccgg ctgatgagtt gggccatctt cacccacacg
      721 aaaggcatca tgcgaccaga taaacttcac cggttgctcc attaatgcag ccatgcgaac
      781 ggcaggtttc atgtagtcgg aaaatacgaa aaaggttccg caagccggaa caacaccgcc
      841 gtgtaaactc atgccgatac aaagacaagc catagtcaat tccgacacgc cggcctgtag
      901 aaatgaacct gaaaaatcgt tttttgaaaa agcttttgtt tttttcaaaa atccatctgt
      961 tttgtcggaa ttggaaagat cggcactggc agtaatcata ttctctactt gtttggccaa
     1021 ttcgccaaga actgttgccg aagctgcacg agtggatttg ttaggttgtt gaacaacagc
     1081 ctcccagtca acctcaggag cttttcccga aaagaatgat tcgagttttt ttgccaaatc
     1141 aggatgttct tttgcccaag cttctttggc tgtttttttt ctggctacta tttctttcaa
     1201 ttcgtttttg cgcttttcat aaagtgcttt tgtctcttca aaaattacaa acggatgctc
     1261 agggtcgcca cccaaatgtc taatggtttc ttcaaaagaa gcaccacatt cgctgatcgg
     1321 cataccgtgt gtggaagtct gattttcgaa actactgccg tcagccttcc gaattccctt
     1381 gcccatgacg gttttaccga ttattaacgt aggtcgctcc ttttctgctt tggcttccgt
     1441 taaagcttga cgaatttgcg ctgcatcatt tccatttatt tcgataactt tccagcccca
     1501 agctttgtat ttttcagccg tattttcact ggtaactgcc tttgtctcgg tagaaagttg
     1561 aatattattg gaatcgtaaa acataatcag gttgtccaat cccaaatgtc cggcaattcg
     1621 ccctgctccc tgtgaaattt cttcctgaac accgccatcg gaaatatagg cataaatggt
     1681 ttgattcatt acatcgccaa agcgtgcttt taaaaatttt gctgcaatgg ctgctccaac
     1741 agcataagta tgcccctgtc ccagtggccc ggaagtgttt tcgatgccgc gcattacatc
     1801 caattcgggg tgacccggag ttggacttcc ccattgacga aactgtgcca attcgtccaa
     1861 tgtaaatttt ccggaaaaag ccagaacgga atacaacatg gtggacatgt gtccgggatc
     1921 aagaaaaaac cgatcgcgac tttcccacgt aggattttca ggatcgtact ccagaaattc
     1981 ggaaaaaagt acgttcataa aatcggcacc gcccattgct ccgccgggat gaccagattt
     2041 ggccttttct accatagctg ctgccaaaat acgtatattg tcggctgctt tgtttaaaag
     2101 ttcaattgaa ttcataataa aaaaattttg ttgttaggtt gtgtttgtta ttcatgtttt
     2161 tttagaagtg atatccgatg gtataattaa agcctgtgtt ggaacttccg gttttcccat
     2221 agcccggaat gtaccacgga gtgatgttgc cggaagtgtc ttccttaaaa aaatgccggt
     2281 tcctgatatt ccaccccagg taaaaatttt tataaacctg tacctgtatt cctactataa
     2341 tctcgtacca cgttttggtt gcggactgac cttcaaaatt taatgtttga ctgccacccc
     2401 aataatcatc cgaaagggaa aggttagtta aatcgtagct gaagttggac catcccaaac
     2461 gaactccgcc cgtaagcata ttgttaatta attctttatc attactttgt ttcagtaaac
     2521 cgaaatcaat gcctattctt tgaaaaattc cgttggtatt aaaacggact tcattggatg
     2581 aaagtttatc ggctccacca aaacctattt cgtaaacagg aaaaattttt cttttcaaat
     2641 tgaactgaac actcccctcc atgccgaaat gttcggtatc ggaaaatcct gtaattaccg
     2701 gagacgccac atccattctg acaagaaagc cgttccacca tttcacttca ggtgatttcg
     2761 ccgatgccgg ggttttattc tgtgcggtag aaaaatttac agccacagca acaaacaaac
     2821 taattatgaa atatctgtaa atgttctgca tcgatggttg aaacattatg ttcttcaatt
     2881 tttaccgaat caacaaaatg attggttatc agaaccgtat ctatcaaatg atttctgatc
     2941 attccacatt caaacgacaa atactggtca taattggtat ggaaaacggt aagtgtatcc
     3001 tgaatgctat cgttgaaacg aataataaat ttcgattcgt ttgaaaattt gttgagcggc
     3061 aatttaatga cagaaacatt tttagaattg ttgtaaagaa tagaatctac gatattgccg
     3121 agagaatcaa ctccgtttac cgtaaggctg tcgatggcaa gcgacgacga agtgtatttg
     3181 cctgttgaac tgttgaaaac agcagtataa aaaccaacct tgagacggga atttttttct
     3241 tttcgacaaa cttcatcgga aacattacat gatgtcgaaa aaaacaaaac agcagtaatc
     3301 caaatgaaaa ctgttgattt ggtaaagttg ttttgaagaa aatttttcaa agtgttaaat
     3361 aaattcttaa agttaataaa aatcattgtt accgctttct cttttgaaag ttgtttgcat
     3421 taggatggaa aatattttcg cacttcttcc ttcaacttcc gaatagcttg ttcggtgggt
     3481 gtttcttcac tggctgcata aatttccaga attttttcgg ccagcaaaga agcaggctca
     3541 ttcggattgc attgtgaagc gcacatatta atcaaacgaa gtaaactttc ctgagctgaa
     3601 gaaatataat tccaaagttc atcactcacg taaatttgtt gcgaaagatt atgtgcaaat
     3661 tcatggcgga tagtgttcaa caattgcgac tgaagttcca agttggtcat gccgggtttt
     3721 atggtgctta gtatcaaatt atttggaatt gtacgctcca aaacaagcat taatctttcg
     3781 taggctctca aacgaatggg cgtaataaca gaataattgc ttttaaacaa ttcgaaattt
     3841 cgccgttttt cttcgttttt caaaagtctg tctagcaaaa ggtaagctgt cagcaaaacg
     3901 agcagcgcag gaatcagcaa tttcaatatt tcggccatga agattggtat gtttttagaa
     3961 gatattctat tttttattcc caaatttgtg cagtttcacc tcaaccggct gcagagtaac
     4021 cagacaacca ttcggaatat ttttcaattt ttcttctatc gttcgataaa agctctcaag
     4081 tttttcggtc gtatccatca tttcaaccac aacggggagt ttttccgtca actcccaaaa
     4141 acgcgaagtt gaaatttttt gactgctcaa tccataaccc ataattccgc gataaactgt
     4201 tacgccagca atgccgtttt cctttgccag aaaaaccaga tgttcgtaca gcagctcgtt
     4261 tcccatttta tccgtactgc tggcataaat tttcaatatg ctgttttcgt tttttttcat
     4321 tttacaagaa attggttact gcatagccaa gaaaaaccgc aagaaaacct aacaaaagac
     4381 tgatgaccgt ataaacggct gcactcagaa attgtccgtt ttgaatcagc tgcaaatttt
     4441 cattggtaaa ggacgaaaaa gtagtaaaac ctccacagaa tcctgccatc agaaacaatc
     4501 gcatgtcagc cgataataat tcacttttgc tggccaaacc cgttaaaatt ccaatcaaaa
     4561 aactgccgga aacatttacc gtgagcgtac ccagcggtaa ggataaaaaa ttttccagca
     4621 aattgattct gtaaacaaga tagcgagcca ttgttccgaa aaaaccacct aatccgacaa
     4681 tcagcaattg cttcatattc taaatatttc cttttttgca aggtactaaa aagctccctg
     4741 tatccgagcc aatgaaagcc attttcttaa atttcctttt cgatcagata gttgtttctt
     4801 tccgacgaat ttcgtgtaat cagttcagca acaaaacctg ccaggaagag ctgcgttcct
     4861 aaaatcatag ccagaatggc cagataaaaa tacggcgaac ttgtaaccag tggagcagaa
     4921 acatgtgccg acaaggcgat aagtttttta gcacctacca caatcaccgc tatcaaaccc
     4981 aacaggaaaa tcaacgtccc tatcaggcca aaaaagtgca tgggctgctt cccgaatttt
     5041 gacagaaacc aaagtgtcat caaatcgaga tatccgttta caaaacgatt ccagccaaat
     5101 ttggtcgttc cgaactgacg cttacgatgt tccaccactt tttcgccaat ttttgtaaaa
     5161 ccggcaattt tggcaagata aggaatatat cgatgcattt cgccatacaa ttcgatactt
     5221 tttaccactt cattgcgata agctttcagt ccgcaattca tgtcgtgaag ttgaagtttc
     5281 gtaacacgtc gagctgttgc attataaatt ttagaaggaa aatttttggt aagtttggca
     5341 tcataccgct tctttttcca acccgaaacc aaatcataat tttgattttt tatcatatcg
     5401 tacaaagacg gaatttcgtc ggggctatcc tgcaaatccg catccatagt aatcaccaca
     5461 tctcctttgg ctgcctgaaa tccaaagtgc aaagctaccg atttaccgta attgtttctg
     5521 aattttatcc cgtgaacatg ctcgggagaa gaggctttca acgattcgat aatttcccat
     5581 gaacgatcgg tacttccatc attcacaaaa attacttcgt aactaaagcc attttcattc
     5641 attacccgct caatccactc gtaaagggga cgaattgatt cttcttcgtt atataaaggt
     5701 acaacaactg atatgtccat ttttaaattg agttttataa attggctttt taaagcacta
     5761 ccggatcaaa taatgcaatt ttaaaaaaat cactcaagca tctgatataa aagaaacaat
     5821 ttgacaaaaa tcaatagaat ttctttattc ttcaaaaata tccttatcct ttttcacaaa
     5881 tgccgacata accagcgcca gcaaagctcc cagcaatacg tttacccaaa tgctctgcaa
     5941 agtataaacc gaaggtttca tcattttttc catttgggta taatattcat cgtccaaagg
     6001 taaattcata ctttctacta ctttcatact ttgctgcaaa acattggaaa gatattcggg
     6061 attgataaac tgaaaataaa caaatttaac aatggccgaa ataatggaag caaagaaaaa
     6121 agttagtaca ataaaggaaa acgatttccc gtaagtgata taaccgccac actcgttatc
     6181 gcgatacttg accgaatagc gatacataac aatgacaatc acaatggtta taaaaaatga
     6241 gacaattctg aaaatggcac cctgagggat ggaaataatg aaattgacgg aaaaaagtat
     6301 tcccatcatt aatccatata tcatggaaga tttgaaaata tttccctgca tgattttatt
     6361 ttttacattt actcatgcaa aagtacaaaa aaagacctga atcggaaata ttaaattttt
     6421 cagttcaatt ataagtcgag aaaacaacac ttaaattcaa tttgtattag cccgtaataa
     6481 ccgagcacat taattgtatt ttttttatta ttttttactg ctcggttttt ttgagggtta
     6541 atatagaaca taatgatgat tttgttgtta aagaaatatg atgtttatgc agaaaaatac
     6601 aatttggaaa gagaattcca acaaaattat ataatctcta atgaggattt ttgttttaat
     6661 tttaatgata aggaatcaaa ttttagtact caaaaattat ggacaaaaca caaaagcaaa
     6721 ttttagaagc tcaaaggact gaactgaccg agcatttgat ttacaaaaaa ctcgccgatg
     6781 attgtaaaga tgagaataat gcaaaaattt tgtatgaaat cggacaacag gaactcgagc
     6841 atgcccatta ttgggaaaag aaaaccggta tcaaagtgaa acctgattat gccagaatac
     6901 gtagaactgt atttatggcg cgtttgttgg gactctcttt tgtgctgaaa caaatggaaa
     6961 aaagagaagg aacgggttca aaattgtatg atgaattgtc ggctgtttac cccgaaacca
     7021 aattgttttc cgagcaggaa aatgctcatg aacaacaatt actggccatg ctggatgaag
     7081 aaagactgca atatgtcggt tctattgtat tgggattaaa tgatgcgttg gtagaattga
     7141 ctggagcttt ggccggcttt acgctggctt tgggaaatac aagaactatt tcgctggcag
     7201 ggttagttac gggaatttct gctgcgcttt ctatggctgc ttccgattat ctttctacaa
     7261 aagccgaagg agatactcgt gcagcaaaat cggcagcata taccggcgtt acctattttt
     7321 tgacggtaat tttgctaatc cttccatttt tgctgatgcc caacaaattt ggagccttag
     7381 cgctaacgct ttcgattgcc gttttaatta ttttctgttt taattattat atttctgtag
     7441 caaaaaatct ttctttcaag ccacgtttca tcgaaatgac aaccatcagc ttgggcgttg
     7501 ctgctttttc tttctttatc ggttatctgc tgaatcaaat tttaggggta aatgtttaaa
     7561 attcatttac tcgctttttg agataaaccg taagtaa
//
LOCUS       DAOSIB010000088         7094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_9634_length_7094_cov_10.633613, whole genome shotgun sequence.
ACCESSION   DAOSIB010000088 DAOSIB010000000
VERSION     DAOSIB010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7094
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9634_length_7094_cov_10.633613"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLD44_07570"
     CDS             complement(<1..440)
                     /locus_tag="PLD44_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HOF99225.1"
                     /translation="MKKELILELFQKFENICYLYNDVECWSARELQALLGYSKWENFE
                     KVIVKAKESCSNAGENIEDHFPDVRKTIPMPKGAEKQIDDILLTRYACYLIAQNGDAR
                     KEEISFAQNYFAVQTRRAELVEKRLLEFERVKAREKLSQTEKQLS"
     gene            complement(451..1137)
                     /locus_tag="PLD44_07575"
     CDS             complement(451..1137)
                     /locus_tag="PLD44_07575"
                     /inference="COORDINATES: protein motif:HMM:NF020052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HOF99226.1"
                     /translation="MAKSFNEIFESFTSVMELLRNEQFQKLLVDYERAKKVFLVGYEV
                     KDDVSSMVLFEAEGRYGLKPTDYLLAFSEFVKRKENEIAAIGILLNKPNDWNTKALNE
                     LKQKLKENNYDEANLQKAHKFVYHKETVDIISMIKHAAKDTEPLLSPDERVNQAIQKV
                     TAGKNLNEEQQKWMEYIKEHLKQNLTLDEDDFKELPVFTDRGGLNKFKKVFADEYQKI
                     IKEINNAIAA"
     gene            complement(1137..3164)
                     /locus_tag="PLD44_07580"
     CDS             complement(1137..3164)
                     /locus_tag="PLD44_07580"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOF99227.1"
                     /translation="MTYESEKITRKKRIDKQLKASGWLIIPYSEGMDLTILTNHAIEE
                     LQTNSGPADYALVVNGKLLGVVEAKKLEVGAANVLEQAKRYSKGANKTVGEWNEYRVP
                     FLYSSNGELIYYLDARDSKNLSRQIYSFHTPEAMETLFNSNKETALQWLKEKPINTPG
                     LRPYQKEAIQAFENNLEERKRLMLLAMATGTGKTFTTVNLVYRMLASGYAKRILFLVD
                     RKSLAAQAVTAFASFDTPRNIKFKDEYELYSQRFKKEDFEGEAYDPTVLPNSYLTNPD
                     GTKTFLYICTIQRMAINLYGKAGAFGENEENEDDAEKLSIPSHAFDLIIADECHRGYT
                     SKETNVWRNVLNHFDAVKVGLTATPASHTVAYFNKPIFNYSLQQAIEEGYLVDYDAVA
                     INSEVLMNGAFLKEGEQVGMIDTETGREQIDQLEDEREFTSTEIEEKITAPDTNQKIV
                     EELKKYTDEWEKRTGRFPKTLIFASNDIPMISHADRLVSICKSVFNRGDDFVAKITGS
                     PTVDRPLKKIKEFRNRPNPKIVVTVDMLSTGVDIPSIEFIVFLRPVKSRILWEQMLGR
                     GTRKCDEIGKTHFVIFDCFNGTLIKYFKDASYNFKIDPPGTETITIKELIEKIYNNED
                     RKANAKRLAKRLHRIEKDMSGNARDKFATYIPNGDMGKFAELLAKFFEKYK"
     gene            complement(3561..4295)
                     /locus_tag="PLD44_07585"
     CDS             complement(3561..4295)
                     /locus_tag="PLD44_07585"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HOF99228.1"
                     /translation="MQVVFSPYKIPEFNLAAEEFLFSEKTDEYLLLYVNKPCVILGSN
                     QSVTNEVNEDFCHLNGISIFRRLSGGGAVYHDEGNLNFCFVFNKKKGYSPLSSDFLQP
                     VISVLNSMEIPVEVGKRKDLWLPDGNKVSGTASHVSKCRELHHGTLLYDVDMEKLQKS
                     LIPTSATNKKAIRSVPSPVENIRSFLERSTVRTQDVNDFFITFTKLMLAYFQIRELSL
                     LLNEDIKKIYRLSISKYGRKEWNYRI"
     gene            complement(4327..5625)
                     /locus_tag="PLD44_07590"
     CDS             complement(4327..5625)
                     /locus_tag="PLD44_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOF99229.1"
                     /translation="MKQLDVRLMHPVEQINVIIKRIYKSGMTTTSGGNISIRDQNGDI
                     WITPSGIDKGSLTFNDIMCVKKDGTVIGPHKPSSEFPFHKAIYETRPELTAIIHAHPP
                     ALVAFSIAHKVPDTKIVPQAHNICGEIGFAPYGTPGSDDLGKKIANEFRNTQYKAVIM
                     ENHGVVLGGMDLMDAYQRFETLEFCCRTIVNASRLGEVNYLSDEQVARYVNHLPSNIP
                     YFMNVDYPSDERALRSEMVKIIRRACDQGLMISTYGTVSVRWRDDDFLITPKNVARWD
                     IVPEDIVQIKKGMAEVGKTPSRSVALHQRIYQLNPQINSIICTQPVNLMAHAITGTKF
                     DVRTIPESWIFLQDVPSIPFGLIYNDIDSVAEMFNHNKVILVENDSVFVTGDKLLNTF
                     DYLEVAEFSANSLVMASAIGHLCPISDQEIDDLRVAFNVQ"
     assembly_gap    5633..5732
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5737..7014)
                     /locus_tag="PLD44_07595"
     CDS             complement(5737..7014)
                     /locus_tag="PLD44_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOF99230.1"
                     /translation="MKTKAVRLYGKKDLRLEEFELPPIKDNEILAKVVSDSLCMSSYK
                     AAIQGADHKRVPDNVAENPVIIGHEFAGELIEIGSKWADQFKVGDKFSIQPALNYEGG
                     PVGILSAPGYSYPFIGGDATYVIIPNEVMEKNCLLPYHGEGFYPASLAEPLSCVIGAM
                     HANYHLKPGSYEHKMGIVDGGKMAILAGVGPMGLAAINFVLHRKDRRPSLLVVTDIDQ
                     ARLDRAASLYTVDFAASHGIDLRYINTSVMQNPIEELKTLTNGNGYNDVFVFAPVKSV
                     VELADAILGFDGCLNFFAGPSDPKFSAMLNFYNIHYAYTHVAGTSGGNNEDMVEALDI
                     MNEGLDPAGLVTHIGGLNAVSEATLHLPEIPGGKKLIYTHITMPLTSISDFAELGKTS
                     EFFRELSSICARHKGLWNVEAEAFLLANANKSN"
BASE COUNT         2060 a         1453 c         1133 g         2348 t
ORIGIN      
        1 gaaagttgtt tttcggtttg tgataatttt tctctggctt ttacacgctc aaattccagt
       61 aatcgttttt caaccaattc tgccctgcgg gtttgaacag caaaatagtt ctgtgcaaat
      121 gaaatttctt cctttctcgc atcgccattt tgtgcaatta aataacaggc atatcgggta
      181 agcagaatat catctatctg tttttcagct cctttaggca taggtatcgt tttcctgacg
      241 tcaggaaaat gatcctctat attttcaccc gcattagaac aggattcttt tgctttaaca
      301 attactttct caaaattttc ccatttgcta taacccagca gagcttgtag ttctcttgcg
      361 ctccaacatt ccacgtcatt gtacaaataa caaatgtttt caaacttctg aaacaattct
      421 aaaatgagtt ccttcttcat aacttacaca ttatgctgca attgcattgt ttatttcttt
      481 aataattttt tgatattcat ctgcaaaaac ctttttaaat ttgtttaaac cgcctctgtc
      541 agtgaacact ggcaattcct taaaatcatc ttcatctaaa gtcaggtttt gtttcaaatg
      601 ttctttgatg tattccatcc atttttgttg ttcttcattt aaatttttac ctgctgtaac
      661 tttctgtatt gcttgattca ccctttcatc aggacttaac aaaggttcag tatcttttgc
      721 tgcatgttta atcatgctaa taatatcaac agtttctttg tggtaaacaa atttatgcgc
      781 cttttgcaaa ttggcttcat cataattgtt ttctttcagt ttttgtttca gctcgttcaa
      841 tgctttagta ttccaatcat tcggcttatt caataaaata ccgattgctg caatttcatt
      901 ttctttacgt tttacaaatt ctgaaaaagc aagtaaataa tctgttggtt ttaaaccata
      961 tctgccttct gcctcaaaca agaccattga actaacatcg tcttttactt cgtatccaac
     1021 aagaaaaacc tttttagctc tttcgtagtc caccaatagt ttttgaaact gctcgtttct
     1081 gagtagttcc atcactgatg tgaagctttc aaatatttca ttaaacgact ttgccattat
     1141 ttatactttt caaaaaattt ggctaatagt tcggcaaact tccccatgtc gccattggga
     1201 atataggttg caaatttatc acgggcattc ccgctcatat ctttttcaat cctatgaagt
     1261 cgctttgcta aacgcttagc gttagccttt ctgtcttcat tgttgtatat tttttcaatg
     1321 agttctttaa ttgtaatagt ttccgttccg ggcgggtcaa ttttaaagtt ataactggca
     1381 tctttaaaat attttatcaa agtaccatta aaacaatcaa atattacaaa atgtgttttc
     1441 ccaatttcat cacattttct ggttcccctg ccaagcatct gttcccaaag aatgcgagat
     1501 tttacaggac gaagaaaaac aataaattct attgaaggaa tgtctacgcc tgtactaagc
     1561 atgtcaactg ttacaacaat tttcggattg ggtcggttac gaaactcttt gatttttttc
     1621 aaaggtctgt caaccgttgg gcttcctgta atttttgcaa caaaatcgtc acctcgattg
     1681 aaaactgatt tgcaaatact aactaaacgg tcggcatgtg aaatcattgg aatatcgttt
     1741 gaagcaaaaa tcaatgtttt agggaaacga cctgttcttt tttcccattc gtcagtatat
     1801 tttttaagtt cttcaacgat tttctgattg gtgtcgggag ccgtgatttt ttcttctatt
     1861 tcggttgaag taaattccct ttcatcttcc aattggtcaa tttgctccct gcccgtttca
     1921 gtatcaatca ttcccacttg ttcgccttct ttcaaaaatg ctccgttcat aagcacttcc
     1981 gaattgatgg caacagcatc atagtccacc aaataacctt cttcaattgc ttgttgtaac
     2041 gaataattga aaatcggttt attgaaataa gcaacagtat gtgatgcagg cgtagcagtc
     2101 aaaccaacct taacagcatc aaaatgattt aaaacatttc tccaaacatt ggtttctttt
     2161 gatgtgtaac cacgatggca ctcgtcagca ataattaaat caaaagcatg tgatggaata
     2221 gaaagttttt cagcatcatc ttcgttttct tcattttcac caaaagcacc agcttttccg
     2281 taaagattta ttgccattct ctgaatggtg cagatataaa gaaatgtttt tgttccatca
     2341 ggattagtta aataagaatt tggtaaaaca gtcgggtcgt atgcttcgcc ttcaaagtct
     2401 tcttttttaa atcgctgtga atacaattcg tattcgtctt tgaatttaat atttcttggt
     2461 gtatcaaacg aagcaaaagc agtaacggct tgagcagcca atgattttcg gtcaaccaaa
     2521 aataaaattc gtttagcata acccgaagcc aacatccgat aaacaaggtt aactgtggta
     2581 aaagtttttc ctgtaccagt tgccattgct aataacatta agcgttttct ttcttctaaa
     2641 ttattttcaa atgcttgtat tgcttccttt tgataaggtc ttagtccagg agtatttatc
     2701 ggtttttctt ttagccactg taaagctgtt tctttgttcg aattaaacaa agtttccatt
     2761 gcttcgggag tatgaaaact ataaatctgt ctgctaagat ttttactatc tctggcatcc
     2821 aaataataaa tcaattctcc attagaagaa tatagaaacg ggactcggta ttcattccat
     2881 tcaccaactg tcttgtttgc tcctttagaa tatcgttttg cttgctctaa aacattcgct
     2941 gcaccaactt caagtttctt tgcttcaaca actccaagaa gttttccatt aactactaaa
     3001 gcatagtcag caggtccgct attagtctgt aattcttcaa tagcgtgatt tgtcaaaata
     3061 gtcaagtcca ttccttcaga gtatggaata ataagccacc cagaagcttt tagctgcttg
     3121 tctattcttt tttttcttgt tattttctct gattcgtatg tcataattct aaatcgcaag
     3181 gtacaaataa ttctcggtgc aatggcaaaa aaatgtgcca tttgtgggct ggcaatataa
     3241 tttactaacg ctatttttat tttcaatgtt attcatgaat gcttcgcatt tcaaaatttt
     3301 tgtgcggtgg gtaaaaaaat taaaacatat ttggttaagc aatgagtgtc gggttatatg
     3361 tctgtcagtt tcttttttca tttcaagttt aatattccat tttcaattag ctgtgtcgtt
     3421 gtcttttcac cgcttgtgca tacgcaacca caatgaatac aatattcaga catgtgggtt
     3481 ccatcagaca tttaaaaaat aaattatatt ctgatgaaat taatgaacaa tacaatctaa
     3541 ttttttggtt tggaaaagtt tcatatccta taattccatt ctttccggcc gtattttgaa
     3601 atactaagtc tatagatttt ctttatatct tcatttaata aaagagagag ttctcgaatc
     3661 tgaaaataag cgagcattaa ttttgtaaaa gtgatgaaaa aatcgttgac atcttgagtc
     3721 cttacggtcg atctctctaa aaaactgcga atattttcta ctggtgaagg aacagaacga
     3781 attgcttttt tattggttgc tgaggttgga atcagtgatt tttgtagctt ctccatatca
     3841 acatcgtaaa gcaaggtacc atgatgcaat tccctacact ttgacacatg tgaggcggtt
     3901 cctgaaacct tatttccatc cggaagccaa aggtcttttc tcttacccac ctcaacagga
     3961 atttccattg aatttagaac agaaatcaca ggctgcaaaa aatctgaact taacggagaa
     4021 tatccttttt tcttattgaa aacaaaacaa aaattcaaat ttccttcatc atgatacacg
     4081 gcacctcctc ccgacaatcg tcgaaaaatg gagataccgt ttaagtggca aaaatcctca
     4141 ttaacttcat tggttacgga ctgattagag cctaaaatta cacatggttt gtttacataa
     4201 agcaaaagat attcatctgt tttttccgaa aaaagaaatt cttcggcagc caaattaaat
     4261 tccggtattt tatatggaga aaaaacaacc tgcattgata aaattcagac tttttttatc
     4321 ctatccttat tgtacgttaa atgccactct caaatcatct atttcctggt cgctaatagg
     4381 acaaagatgc ccaatagccg aagccattac aagtgaattg gctgaaaact cggccacttc
     4441 caaataatcg aaagtgttta acaatttatc tccggtaaca aatacactgt cgttctccac
     4501 caaaatgact ttgttgtggt taaacatttc agcaacgctg tcaatatcat tataaatcaa
     4561 accgaaagga atggacggaa catcctgaag aaaaatccaa ctttcaggaa tcgtgcgcac
     4621 gtcaaatttt gtaccggtta tggcatgtgc catcaaattt accggttgcg tgcaaataat
     4681 ggaatttatc tgtgggttca attgatatat tcgttgatgt aaggctaccg aacggctagg
     4741 cgttttacct acttcagcca ttcctttttt aatctgcaca atatcttcgg gaacaatatc
     4801 ccaccgtgcc acatttttcg gcgtaatcaa aaaatcgtcg tctcgccaac gtaccgacac
     4861 tgtgccgtag gtagaaatca taagcccctg atcacatgct ctccgaataa tttttaccat
     4921 ttccgaacgc aaggctctct cgtcggaagg ataatccaca ttcataaaat atgggatatt
     4981 ggaaggaaga tgattaacgt atcttgcgac ctgttcatcc gataaataat ttacttctcc
     5041 taatctactt gcattcacaa ttgtgcggca acaaaattca agtgtttcaa aacgctgata
     5101 ggcatccatc aagtccatcc ctcccaaaac aaccccatga ttctccatga ttaccgcttt
     5161 atattgcgta ttcctgaatt cgttagcaat tttctttccc aaatcatcac tccccggtgt
     5221 cccataagga gcaaaaccta tttctccaca aatattatgg gcttgaggaa ctatttttgt
     5281 gtctggaact ttatgtgcta tactaaaggc tacaagtgca ggaggatggg cgtgaataat
     5341 tgctgtcaac tccggacgcg tttcgtaaat ggctttatga aagggaaact cagaagaagg
     5401 tttgtgcgga ccgataaccg ttccgtcttt tttcacgcac atgatatcgt taaaagttaa
     5461 cgatcctttg tctatacccg atggagtaat ccatatatcg ccattttgat ccctgataga
     5521 aatatttcca cccgaagttg tggtcatacc actcttgtaa attcttttga tgatcacatt
     5581 gatttgttcc accggatgca tcagcctaac atctaattgt ttcatattct atnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntaatttaa tttgatttgt ttgcatttgc
     5761 caacaaaaaa gcttcagctt ccacattcca taatccctta tgtcttgcac aaatggaaga
     5821 caattctctg aaaaattcgc tggttttgcc taattcggca aaatctgaga ttgacgtcaa
     5881 aggcatggta atatgagtat aaatcaattt tttccctccc ggaatttcag gcaaatgaag
     5941 agtagcttcg gatacagcat tcaaacctcc aatatgcgtt actaaacctg caggatcaag
     6001 accctcattc attatatcca aagcttctac catatcttcg ttgtttcccc cacttgtgcc
     6061 ggctacatgc gtataggcat agtgaatgtt gtaaaaattc aacatggcac tgaacttggg
     6121 atcgcttggt cctgcaaaaa agttgaggca tccatcgaat ccgagaatgg catctgctaa
     6181 ttcaacaacc gatttcactg gagcaaaaac aaatacatca ttgtagccat taccgttggt
     6241 taaagttttt aattcttcta tcggattttg cataaccgaa gtattgatat aacgaagatc
     6301 gataccatgt gaagccgcaa aatcaacggt atagagtgaa gcagccctgt caagacgtgc
     6361 ttgatcaata tcggtaacta ccaacaacga agggcgacga tcttttctgt gtagcacaaa
     6421 attgatagca gccaatccca ttggtcccac accagccaga atagccattt ttccgccatc
     6481 cacaattccc attttgtgtt cataactacc aggcttcaga tgataattgg catgcatggc
     6541 gccaatcaca caagaaagag gctctgccag cgatgccgga taaaatcctt ccccatgata
     6601 aggcaacaaa caatttttct ccattacttc attgggaatg atcacatacg tagcatcacc
     6661 accaataaac ggatagctgt atcccggcgc actcaatatt cctacgggac cgccttcgta
     6721 attcaacgcc ggctgaatgg aaaatttatc gccgacctta aattgatctg cccacttcga
     6781 accaatttca atcaattcac ctgcaaactc atgaccgatg atcaccggat tctcggccac
     6841 gttatcgggc acacgtttgt gatctgctcc ctgtatggcc gctttgtagg atgacataca
     6901 caacgaatcg gaaactactt tagccaaaat ttcattatct tttatgggtg gcaattcaaa
     6961 ttcttccaac cgaagatctt tcttgccgta taaacgaact gcttttgttt tcatattttt
     7021 tatttttaga accaagaacc aagaatcaag acataagaga atttttgaag tcatgtatca
     7081 ttcgctgcaa ttct
//
LOCUS       DAOSIB010000089         7015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_9762_length_7015_cov_11.423707, whole genome shotgun sequence.
ACCESSION   DAOSIB010000089 DAOSIB010000000
VERSION     DAOSIB010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9762_length_7015_cov_11.423707"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PLD44_07600"
     CDS             <1..972
                     /locus_tag="PLD44_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF99231.1"
                     /translation="NVAMEPFRALVADMLPAEQTTVGFSVQSFLIGIGAVIGSWLPYV
                     LAEWLGIAKTAPEGQVPPNVIFSFYIGAVVLIVTILWTVFTTKEYSPEELQAFESEEE
                     RTEKKNPKFSDIFKDMATMPKTMKQLSITQFFTWIALFGMWVFTTPAIAQHVYGLPVT
                     DTSSPTYQDAGNWVGILFGVYNGVAMFYALALPAIARNIGRKLTHSFSLICGAAGLLS
                     IFFINDPQWLILSMIGVGIAWGSILSMPYAILAPSLPPAKIGVYMGIFNIFITVPQIV
                     NGLFGGMVVKRIFDSQAIYALIMSGCFLLIAAISVLWVEDKAEIRHR"
     gene            complement(1100..5011)
                     /locus_tag="PLD44_07605"
     CDS             complement(1100..5011)
                     /locus_tag="PLD44_07605"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOF99232.1"
                     /translation="MRKIIKHIIRTIGFLIITPVILLLLALGALQTPFVKSLLVDFAE
                     LQAEKVLNGQLHIGKLEGNFINGLRLKQILLTNHSDTLIWIPMLKIDYQLAPLVSGNI
                     SIHSLTIDRLQVKLMQQKNGRWNFQELIKTPSQPTEKTSPFKMRITLENLQLNNAQIF
                     ILSENPSLPEKISDLHLKCNGEYSDKQSKLFMEEFRLTSHQPDLQIKELKFNFVQESE
                     ILHLSDFFLRTGNNTLTANSKIDISASETSFLSVKTAPLFLNEFADFFSAPLQENIHP
                     LVHVESIMQDDNLRLTLNLSEKKEKIEINLQVSKITEIIRQPQLTDNVTYTLMANVDQ
                     MELNKWINLSEPNILLNGEIFINGKGISLNTMEMGIKGDFGKLKINNQDLHKLNFNFD
                     YASKKLTGQLFAESPFGNLYLQSAIKEPTESIPSYKFLLVTRKLNPSTLFPAMKGSSD
                     LNMKIELEGSGFDPLQMTAYAKIQVDTSSISGWTIHSLQSNLNFQQQNFFIENFSVET
                     ASAHLSANGIYSIKGNSDAFLNLTLNNASEIIKFTGVENLNGRGKIAGHFHGAPKKFL
                     TDLNLTLFDTQFQTYSAEEVTAHITAQIPNDRNFSVSGNLNAKNLNLGGIELQSIQLV
                     SNADSKNIQLSSIAESGEFNTSFKSAIHLDNPLLVELSDFRINYRNNSWKQGTDTALF
                     YLSETDYRLEHLKLISEDADTLQTLSAEGKISKNGEQNFQISLSNLDFAKISETFFPE
                     QSLQGKINFNAQLMGEASSPVAEAKFRLNNFALQNYYAGNMTGTLQLTQQQLNTKVNI
                     NLPDSGKLSADAKIPVNFRLDSMQFNIPSYENENFDFNLFANKIPLTLLQSFLPDGQY
                     EGIIQTSMHAGGNIKEPELKGKIEITDGKIKMPQYGINYPHFMAGISMDNNKIKIDTF
                     LITSRDGRMNINGNMSPPTFLSKSTNAPSPLANVQIHFANFRPIDHKNYNMAVSGNAL
                     LKGNADSLLFSGDINIPQAEINLPSLLQLVGKTSAPQLPEPLLIKELKKMQPEDSLVI
                     TLRPRNEESKKEETDFLKKLQGNLRVKIPRNTWIKNQDMRVELSGDVQLMKHKEFFEL
                     FGSVEVLRGQYNLLGKVFIIESGTISFQGGEKINPLLNFTAAYNFRDSNRQQKKLLAN
                     ISGEMMSPEIKFTMDNQQIDEGDALSYILFGAKLDALTSGQRENINQTFDALGLTESL
                     AASGLSSQLTKLLSNTLNVDYIEVKSKGSFESGSLEVGKYLTNKIFINYVRNFGNYQR
                     DENFSEYEIRVEYEILKFLFLQLTTSPVKNGIDIVIKPTKK"
     gene            complement(5019..6884)
                     /locus_tag="PLD44_07610"
     CDS             complement(5019..6884)
                     /locus_tag="PLD44_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOF99233.1"
                     /translation="MPYRFKFLEFNIFRQGKAVVLFLFYFCAIAAKAQNGYQIEKITI
                     VGNQTFSKSELLEKTNLATYNFLQRTFLKKEPVIFNENLMELDIERMTRFYQTEGFPF
                     VEVQVDSLATNHKKQKVEIYIKIEENTPIFFGEIGFDIESQSISEEKKDTLLHLIKKH
                     IVSKNGRRFRDKDVHEDKSRIEQIFINSGYVYVQVDHLINLSLDSYRADVVYVIRPFE
                     ICFFGKTEIVGNQNIKESLIRRQLAYTEGEIYSNKKLDKTRKQLFDLQLFRVASLQPQ
                     IDASSTRNSIPVKLTIEETPQWNAQIGLGYGTEDQFRTFLDLSFHSLWGDASRFHLYA
                     KHSALEPYHFSLSWIQPQLFSKNISMRLNPFLRRQAEPGFDIRTYGLNLPFGYSFSDK
                     INLTLSYYLENIKQYSEVDNLELINPESKDFLYNKSGLLTTFQYNNAFPQINPERGEI
                     ISIGVKSNGYLFGSDFDYTKIWIDTRKYLQINNFSCAARLMGGTVFSSNVIEYVPVED
                     RFYSGGSNSVRGWSRWALGPKRDDGSPLGGKSNLELSLEIRHPLFWLLESAVFLDAGN
                     VWASSFHFPLDKLAYSAGTGLRIKTPVGAIRLDISVPLWNEKKSVQVFLNVGQAF"
BASE COUNT         2023 a         1221 c         1372 g         2399 t
ORIGIN      
        1 aatgtggcta tggaaccttt tcgtgcgctg gttgccgata tgctacctgc cgaacaaact
       61 acagtcggat tttcggttca gagttttctg atagggattg gtgctgtcat tggttcatgg
      121 ttgccttatg tgctggcaga gtggttgggc atagccaaaa ctgccccgga agggcaagtt
      181 ccgccaaatg tgattttttc tttttatatt ggtgctgtcg tgctaattgt tacaattttg
      241 tggacagtgt ttacaacgaa agaatactcg ccggaggaat tgcaggcatt tgaatcggaa
      301 gaggaaagaa cggagaaaaa aaatccaaaa ttttccgata ttttcaaaga tatggcaact
      361 atgccaaaaa ccatgaagca gctttccatt actcaatttt tcacatggat tgctcttttt
      421 ggcatgtggg tttttacaac gccggccatt gctcagcatg tttatggttt acctgttacc
      481 gatacttctt ccccaactta tcaggatgcg ggaaattggg taggaatact ttttggcgtt
      541 tacaatggtg ttgccatgtt ttatgcgctg gctttacccg caatagccag aaatataggc
      601 agaaaattga cacacagctt ttccttgatt tgtggcgcag ccgggttgct ttcaattttc
      661 tttatcaatg atccccaatg gttgatactt tccatgattg gtgtcggaat agcttggggc
      721 agcattttgt cgatgccgta tgccattctt gctccatctc ttcctccggc aaagatcgga
      781 gtttatatgg gtatcttcaa tattttcatt actgttccac agattgtgaa tggacttttt
      841 ggtggaatgg tggtgaaacg gatttttgat tcacaagcca tttatgcact tatcatgtcg
      901 ggttgttttt tgctgatagc cgccataagt gtgttatggg ttgaggataa ggcggaaatt
      961 cgacatagat agaatattgc ttagatgaat ttattgtatg aattgtttcg atttttttat
     1021 attttcccga ccttcttcca gcattacata ttgaaaggaa tgagaagcag cttcttttct
     1081 ttaaaagtgg gtacaagcct tatttttttg tgggtttaat aactatgtct attccatttt
     1141 tgaccggtga agtagttaat tgaagaaaca aaaatttgag aatttcgtac tcgactctaa
     1201 tttcatattc actgaagttt tcgtcgcgtt gatagtttcc aaaattgcga acgtaattaa
     1261 taaaaatttt gttggtcaag tatttaccaa cttcaaggga accactttca aatgaaccct
     1321 tgctttttac ttctatataa tctacattca gtgtattact cagaagtttg gtaagttgcg
     1381 aagaaagtcc cgatgcagca agactttcgg tcagtcccaa tgcatcaaaa gtttgattta
     1441 tattttctcg ttgtccactg gtcaaggcat ccaattttgc tccaaacaga atataggaaa
     1501 gtgcatctcc ttcatcaatt tgctggttat ccattgtgaa ttttatttca ggcgacatca
     1561 tttctcctga aatatttgcc agcagttttt tttgttgtct gtttgagtct ctgaaattgt
     1621 aggctgcagt gaagttgagt aatggattta ttttttctcc accttgaaaa gaaattgttc
     1681 ccgactcgat aatgaaaact tttcccagta aattgtattg accgcgcagc acttcaaccg
     1741 atccgaaaag ttcaaaaaac tctttgtgtt tcattaattg aacatcacct gaaagttcaa
     1801 cacgcatatc ttgatttttt atccaagtat tacgtggaat ttttacacgt aaatttcctt
     1861 gtaatttttt taaaaaatcc gtttcttctt ttttgctttc ttcattacgg ggtcgcagtg
     1921 taataaccag agaatcttca ggttgcattt ttttcaactc tttaattaat agaggttcgg
     1981 ggagttgagg ggcggaggtt tttcctacca gttgcagcaa tgaaggaaga tttatttcag
     2041 cttgagggat attaatatct cccgaaaaga gtaaactgtc agcgtttccc ttcagcaaag
     2101 cattgcccga tacagccatg ttataatttt tatggtcaat aggtctgaag ttggcaaaat
     2161 gaatttgaac atttgccaaa ggtgaaggag catttgtaga ttttgataaa aaagtgggag
     2221 gggacatatt accatttata ttcattcggc catcacgact ggttattagg aaagtgtcaa
     2281 ttttaatttt attgttatcc atcgatattc cggccatgaa gtgaggatag ttgattccat
     2341 attgcggcat tttgattttt ccgtctgtaa tttcaatttt tcctttcagt tcgggttctt
     2401 taatgtttcc accggcatgc atggaagttt gtatgattcc ttcatattgt ccgtcaggaa
     2461 gaaaagattg cagtaatgta aggggaattt tattggcaaa aagattaaaa tcaaaatttt
     2521 cattttcgta acttggaata ttgaattgca tactgtccag acgaaaattc accggaattt
     2581 ttgcatcggc agaaagtttt ccgctgtccg gtaaatttat gtttactttt gtgttcaact
     2641 gttgttgggt gagttgaagt gtccctgtca tgttgcctgc ataataattt tgaagtgcga
     2701 agttgtttaa ccgaaatttt gcttcggcta ccggagaaga agcttcgccc atcaattgtg
     2761 cattaaaatt tatttttcct tgtagcgatt gttcaggaaa aaatgtttcg gatattttgg
     2821 caaaatccaa attagagagg gaaatttgaa aattttgttc tccattttta ctgatttttc
     2881 cttcggccga aagagtttgt aaggtatcag catcttcaga aattagtttt aaatgttcaa
     2941 gccgataatc agtttcgctc aagtagaaca gcgcagtgtc ggtaccctgt ttccaactgt
     3001 tgtttcggta atttattcgg aaatcggata attcaaccag cagagggttg tcgagatgaa
     3061 ttgccgattt aaatgaagta ttgaattccc cgctttctgc aatagacgac aattgtatat
     3121 tttttgaatc ggcattggat acaagttgaa tgctttgtaa ttcgattcca ccgagattca
     3181 gattttttgc attcaaatta cctgatacag aaaagttcct gtcattgggt atttgtgcag
     3241 ttatatgtgc tgtaacttcc tctgcactgt aagtttgaaa ttgcgtgtca aacaaggtta
     3301 aattcagatc ggtaagaaat ttttttggag ctccatgaaa atgtccggca attttgcctc
     3361 ttccattcaa gttttctacg ccggtgaatt ttattatttc tgaagcgttg ttgagcgtca
     3421 agttcagaaa agcatcggaa tttcctttta tgctgtaaat gccattggca gataaatgtg
     3481 ccgatgccgt ttcaactgag aaattttcaa taaagaaatt ctgttgttga aaatttagat
     3541 tggactgcaa cgagtgaatt gtccatccgg aaatactgga ggtgtccact tgaatttttg
     3601 cataagcagt catttgaagt ggatcgaaac cggaaccttc aagttctatt ttcatattca
     3661 aatcggaaga acccttcatg gcagggaaca aggttgaagg attcaatttt cgggttacaa
     3721 gcagaaattt ataagatgga atgctttcgg taggttcttt tattgctgac tgtaaataca
     3781 gattaccaaa aggactctcg gcaaataatt gtcctgttaa tttttttgaa gcataatcaa
     3841 aattgaaatt cagtttatgt agatcctggt tgttaatttt taattttccg aaatcaccct
     3901 ttatccccat ctccatcgta ttcaatgaaa tgccttttcc gtttatgaat atttctccat
     3961 tgagcaaaat attgggttca gacaggttga tccacttgtt caattccatt tggtctacat
     4021 tggccatcag agtataggta acattgtcgg tcaattgggg ttgccgaatt atttccgtaa
     4081 tttttgaaac ctgaagattt atttcgattt tttccttttt ttcggacagg tttaatgtga
     4141 gccgaagatt atcatcctgc attattgatt cgacatgcac cagtggatga atattttcct
     4201 gcaaaggagc agaaaaaaaa tctgcaaatt catttagaaa caagggagca gttttaacac
     4261 ttaaaaagga agtttctgaa gctgaaatgt cgattttcga attggcagtc aaagtattgt
     4321 tgccggtacg aagaaaaaaa tcggaaagat gtaatatctc actctcttgt acgaaattaa
     4381 atttcagttc tttaatttgt aaatcaggtt gatgtgaggt tagtctaaat tcttccatga
     4441 aaagtttcga ttgcttgtcg gaatattccc cgttgcattt cagatgaaga tcggaaattt
     4501 tttcaggcag ggagggattt tctgaaagaa taaaaatttg agcattgttc aattgtaaat
     4561 tttcaagtgt tattctcatt ttaaagggag atgtcttttc ggtgggttgt gaaggggtct
     4621 ttattaattc ctgaaaattc caacgcccat ttttttgctg catcagtttt acttgcaacc
     4681 gatcaattgt taaggaatgt atcgaaatat tacctgaaac taaaggtgca agttggtaat
     4741 ctatctttag cattggtatc catatcaacg tgtccgaatg gttggtcaac aaaatttgtt
     4801 ttagacgtaa accattaata aaatttccct ccaacttacc tatgtgtaat tgtccgttca
     4861 ataccttttc ggcctgaagt tcagcaaaat caaccaaaag ggattttaca aagggtgttt
     4921 gtaatgcccc caacgcaagt aacaacagga tcaccggagt tatgattaaa aaccctattg
     4981 tacggatgat atgtttgatg atttttcgca taaggttttt aaaatgcttg tccgacattc
     5041 agaaaaactt gtaccgattt tttttcgttc caaagcggaa cgcttatatc caagcgaata
     5101 gcgccaacag gtgttttaat tctcagtcct gtacctgctg agtaagccag tttatccaaa
     5161 ggaaaatgga atgaggatgc ccacacatta ccggcatcca aaaacacggc actttcaaga
     5221 agccaaaata aaggatgtcg tatttccaaa ctgagctcca aattgctttt tcctcctaat
     5281 ggagaaccgt catctctttt gggaccaaga gcccagcggc tccagcccct tacagagttg
     5341 ctccctccag aataaaatct gtcttccact ggaacatatt ctattacatt ggaggaaaaa
     5401 acagttccgc ccattaatcg tgcagcacaa gaaaaattgt taatttgcag atattttcgt
     5461 gtatctatcc aaatttttgt ataatcaaaa tcactcccaa ataaatatcc attgcttttt
     5521 acgccaatac ttataatttc cccacgttca gggttaattt gcgggaaagc attgttgtat
     5581 tgaaaagtag tcagcaatcc cgatttgttg taaagaaaat ctttactttc aggattgatt
     5641 aattccaaat tgtccacttc ggagtattgt ttaatatttt ccaaataata acttaaggta
     5701 agatttattt tatcggaaaa ggaatagccg aatggtaaat ttagtccata agttcgaata
     5761 tcaaaaccgg gttctgcctg cctgcgtaaa aaagggttaa gccgcatcga aatattttta
     5821 gaaaacaatt gcggttgtat ccagcttaaa ctgaaatgat acggttccaa tgccgaatgt
     5881 ttggcgtaca aatgaaatcg tgaagcatcg ccccacaacg aatgaaagga cagatcaaga
     5941 aaggtacgaa attgatcttc tgtaccatag ccaagtccga tttgagcatt ccattgggga
     6001 gtttcttcaa tggttaattt tactggaatg gaatttcgag tggaggaagc atcaatctga
     6061 ggttggagcg aggccaccct gaaaagttgt aaatcgaaaa gttgttttcg tgttttatcc
     6121 agtttcttat tgctgtaaat ctctccttct gtgtaggcga gttgacggcg aatcaaactt
     6181 tcttttatgt tttgattgcc aacaatttcg gtcttcccaa aaaaacaaat ttcgaatggt
     6241 cgaataacat aaacgacatc tgcccgatat gaatcgagtg aaagattaat caaatgatca
     6301 acttgtacat atacatatcc tgagttgata aatatttgtt cgatgcggga tttgtcttca
     6361 tgcacatctt tgtctctgaa acgcctgccg tttttagata caatgtgttt ttttataaga
     6421 tgtaatagtg tatccttttt ttcttccgaa atagattggc tttctatgtc gaatccgatt
     6481 tctccaaaaa atatcggggt gttttcttcg attttaatat aaatttcgac tttttgtttt
     6541 ttatgattgg tggccaatga atccacttgc acttcaacaa acgggaaacc ttcggtttga
     6601 taaaaacggg tcattcgttc aatatccaac tccattaaat tttcgttaaa aattaccggt
     6661 tcctttttca aaaaagtacg ttgcaaaaaa ttgtaagtag caaggttggt tttttccagt
     6721 aattccgatt ttgaaaatgt ttgattgccg actatcgtta ttttttcaat ttgatatccg
     6781 ttttgagctt ttgcggcaat ggcacaaaag taaaacagaa ataaaacgac agcttttccc
     6841 tgccgaaaaa tattaaattc caaaaatttg aatcgatatg gcattcctat gtcctaaaaa
     6901 tttaaacatc ggggttttgc agacgtgatg acaaaaaaaa atgctgttta attgggaaaa
     6961 taatttcatc agaatataat ttatttttta aaggtctgat ggaacccaca tgtct
//
LOCUS       DAOSIB010000090         6998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_9791_length_6998_cov_12.004897, whole genome shotgun sequence.
ACCESSION   DAOSIB010000090 DAOSIB010000000
VERSION     DAOSIB010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6998
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9791_length_6998_cov_12.004897"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..879)
                     /locus_tag="PLD44_07615"
     CDS             complement(64..879)
                     /locus_tag="PLD44_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99234.1"
                     /translation="MAGNEIVLNPGFEVAINAKFEALTGNDYRYRLSVITYNIKKGHY
                     SGNGNVIHESGADVAAVQEIRFGFNNFKNLKNSAKMEGVGCATIGSTTVGYGIALLWK
                     SWLGTPTICKDTVHVTDSDEIRGYIIAEWSDFAVISTHLSTNENSRKKMANKILAESI
                     VQNCISTGKPIYFAGDANEEPHTTTGAVNIFNNAGFDVLNNPARNYQYNHNGVWGYWY
                     DDATTAKGKMIDLIMEYNTNSNKELLERGIPSCADFNYVLSDHLPYLVKVKIQ"
     gene            complement(1072..2289)
                     /locus_tag="PLD44_07620"
     CDS             complement(1072..2289)
                     /locus_tag="PLD44_07620"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HOF99235.1"
                     /translation="MRKIMFILGILLATQFMTAQLKVKSDGKIEFGNSPVSTFGNIYA
                     VILEGNRVGLEAHRRGQGDTSPTWGRAIASSSEISTYRGDIGVDAYTISATPLTSGRA
                     IGVRGTAGNATNGYNYGVMGNLTGTNNGAGVIGTVEDYYGSELDGRYAGYFKGNVKVT
                     GTVNGIIINGSDTRYKQNIVDLSSFSTDALTGKSKTGATLSTILQMNPVQYNLKQVYF
                     ESKSDTATVSRGFFDENSQMFQKKHYGLIAQDLQKLYPDLVYEDDNGYLAINYTGLIP
                     LLIQSIKELNVKIEALESREAISMQKAKGNNMDSKDDISDNLLTAMLYQNAPNPFSQS
                     TQIKYYLPEEVGLAYLCIYNMQGEQLKQIAITDRGEGQQTISGSEFPAGMYLYALLAD
                     GKEVDVKRMILTK"
     gene            3375..3698
                     /locus_tag="PLD44_07625"
     CDS             3375..3698
                     /locus_tag="PLD44_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99236.1"
                     /translation="MKKTAFENIFGSFSYQQILPELMFAYEQKPFLNKQSLLFATPEK
                     AILDLLYLYPQYNNEQEIKELRFDEAFMQEDLNTERLNEFAEKFQSKVLRKRVDLLLK
                     IHDLC"
     gene            3692..4477
                     /locus_tag="PLD44_07630"
     CDS             3692..4477
                     /locus_tag="PLD44_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOF99237.1"
                     /translation="MLNLEQIQNYFPPQIKENPAHKKYMLKEYIQLMILNFLATSKFV
                     KKITFIGGTNLRLVKGIDRFSEDLDFDCKDFNAEYFAEMSQSVLTFLNRSGIQAESRE
                     KESKKLTAFRSRIYFPELLFELGLSAYREERFLIKLESQNQEFEYTPQMTTISACGFF
                     MSFPVPPDDVLCAMKLSALLSRAKGRDFYDAMFLLAQTKPNYDFLAQKQAIHDQVELK
                     TKLIEIIEKVDLEHKSKDFEHLLFNKENKNKIVNFSEFVKNLN"
     gene            5126..5284
                     /locus_tag="PLD44_07635"
     CDS             5126..5284
                     /locus_tag="PLD44_07635"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF99238.1"
                     /translation="METNRIEYKQKLTDQLEKEVVAFLNYAGGGIIYLGIDNIGNVIG
                     VTGIGTFF"
     gene            5331..6233
                     /locus_tag="PLD44_07640"
     CDS             5331..6233
                     /locus_tag="PLD44_07640"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF99239.1"
                     /translation="MKFEQLRIYYDSIDKPLNSQFAKTLELLNEEAQYNYVAYLMADN
                     NTISVKLAKYRGVNRVDLIQSDEYGFGSLIKAIKSVLDKIEVENTTFTKITSKERIEK
                     RRWNSLAMREAVINAMAHNDYTYELAPKFEFFDDRFEITSYGGLPQGLSENEFFQGIS
                     IPRNKEIMRILRDIELVEQLGSGIPRILQSYGKECFQFSDNYIRISFPAEYDVDECEE
                     KDTTKHIEVSEDTNVGVNVGVNVGVNELFEIIKKYPGKNSKELHLYFDVSNRTIERWL
                     KTLKKERRIEFIGAPRIGGYFIVK"
BASE COUNT         2170 a         1268 c         1250 g         2310 t
ORIGIN      
        1 tattcaccga taatatgaat gacaaacaaa ggataattga taagagttgt ttcataattg
       61 gaattattga atttttactt taacaaggta gggcaaatgg tcacttagta cataattaaa
      121 atcagcacag gacggtattc ccctttcaag gagttcttta tttgaatttg tgttgtattc
      181 cattatcagg tctatcattt ttccttttgc tgtcgtggca tcatcatacc agtagcccca
      241 tacgccattg tgattatatt gatagttgcg cgccggatta ttcagcacat caaaaccggc
      301 attgttgaaa atattgacag caccggttgt tgtatggggt tcttcattcg catctccggc
      361 aaaataaatc ggctttcctg tacttatgca attttgcacg atactttcag ctaaaatttt
      421 atttgccatc ttttttctgc tgttttcatt ggtggataaa tgagtagaaa taacggcaaa
      481 atcactccat tcagcaatta tataccctcg aatttcgtcg ctatctgtta catgtaccgt
      541 gtccttacaa attgttggag tacctaacca agatttccat aatagagcaa tgccatatcc
      601 tacagtggtg ctccctatag tagcacatcc taccccttcc attttggctg agtttttcag
      661 atttttaaaa ttgttaaaac cgaaacgtat ttcttgtacg gcagccacat ctgctccact
      721 ttcatgtatt acgtttccat ttccgctata atgtcctttt tttatattat aagttattac
      781 acttaagcga tatcggtagt cattgcctgt taatgcttca aatttggcat ttattgccac
      841 ttcaaacccc ggatttaaca caatttcatt accggccata tatgttgttt tggcatgaag
      901 gctctgtgtt gattggatac aattactggc tctatacgta gcgggttcta tcatcgtttg
      961 ggaaaggttt aaatttacgg gaatgtcgcc ttgtgaattt gttttccaca gacacaaggt
     1021 tgttaaagta attattagta ttgtttttat tgttttcacg gctcaatcct cctattttgt
     1081 taaaatcatt cgtttgacat ctacttcttt accgtcagca agaagtgcat acaagtacat
     1141 tcctgcagga aattctgaac ctgagatcgt ttgctgtcct tcacctcgat cagtaatggc
     1201 aatttgtttg agttgttctc cttgcatgtt gtaaatgcag aggtacgcta atccgacttc
     1261 ttcaggcaga taatacttaa tttgtgtgga ttgagagaat ggattgggag cgttttggta
     1321 aagcatggca gtaagcagat tgtccgatat gtcgtctttt gaatccatat tattgccttt
     1381 ggctttctgc atagaaatag cttccctact ttctaacgct tctatcttta cgttgagttc
     1441 cttaattgat tgtatcaata atggaattaa gccggtgtag tttatagcca agtatccgtt
     1501 gtcatcttca tataccaaat ctggataaag tttttgtaaa tcctgcgcta tcagtccata
     1561 atgttttttc tggaacattt gggaattttc atcaaaaaaa cccctgctta cggtagcagt
     1621 atcactttta gattcaaaat aaacttgttt caaattgtac tgtacaggat tcatttgtaa
     1681 tattgtgctt agtgtggcac cggtcttgga tttaccggtt aaggcatcag ttgaaaaact
     1741 gcttaagtca acaatattct gtttataacg ggtatcggaa ccattgatta taatcccatt
     1801 cactgttccg gtaaccttta cgtttccttt aaaatatccg gcatagcgcc catccaattc
     1861 acttccataa taatcctcaa ctgtaccaat aacaccagct ccattatttg taccagttaa
     1921 atttcccata acaccgtaat tataaccgtt tgttgcatta ccggcagttc ctcggacgcc
     1981 aatcgctcgt cctgaggtca gtggagtagc actaattgtg taagcatcaa ctccaatatc
     2041 tcccctatat gtcgatattt cagaagaact tgcaattgct ctcccccatg tggggctggt
     2101 gtcaccctgt cctcttctgt gtgcttctaa acctacccta tttccttcaa ggataaccgc
     2161 ataaatgttt ccaaatgtgg aaactggtga atttccaaat tctattttgc catcactctt
     2221 tactttcagt tgtgctgtca taaactgtgt tgccagcaaa attcctaaaa tgaacataat
     2281 ttttctcata attttaaaat ttaaatgatt aatgtaagat tactactagt gtcccttgaa
     2341 aattgggacg aataaaaaat taaataaatt tggctcatca agcctaaaac gttctttgat
     2401 tttttgaaat ttggttttat cggttttatt gacattttta atgagtcctt cttggggaat
     2461 tgactccaca taaacagcgg cgatgccatg gggatttagt gactgaacat gaagctgagc
     2521 tacaatcgag aactgatata ctaaaaggaa gaaaactaca gataatattt tcatgaagcg
     2581 taagatactt tttgtcttaa ttttgttgtt gtaatcaatg tttttaagct tgcaaaagta
     2641 ggaattaatg tggttttttc aaaatctttt ttttctggct gtcaatcatt cttccacaag
     2701 aatttattta ggcattgaca acctgcaata cgaaacgcgg aactcgtacc ccggaaccca
     2761 atcatacaat cagaagaaaa aaccttattt tacaccttat tttcaaacta aacaagctta
     2821 gtagagttga atgactattt gattttgacc ttattagctt attttgcggg ttgttgaact
     2881 aattgccttc gagagcctca ggcaacgaat gctcccggtg gctgattttc ggtgccggtg
     2941 gctgaggctc tcgaagccac gtcattgttg gtccgcgggc tgttgatgat tcttgtattt
     3001 gacaatattt atttatcata attttgtttt agtctttttt ttatgtaatt ttgctgaaaa
     3061 ttagaagcaa aacggaaatg aatttttggc aattaaggaa tcaatttttc gacttggtat
     3121 gtttcaatat caatcaagtt tatgcgtggc aaccgaattt tgagaaaaat aatctgactc
     3181 gttgggtaaa gcaaaacttg cttgtgaaat tacgcaattc gtggtatagt tttcctgagt
     3241 atttaaaaac acctaatatt cagtattttg tgtcgaacaa aatctatagt ccttcctatg
     3301 tcagcttgca tgctgcattg gcattttatg gcataattcc cgaagcaatt gtccaaacga
     3361 ctgctgtaag ttctttgaaa aaaacagctt tcgagaatat tttcggttca ttttcatatc
     3421 aacagatttt acctgaattg atgtttgctt atgaacaaaa accgttttta aacaaacaaa
     3481 gtttgctttt tgctaccccc gaaaaagcaa ttttggattt gttgtacctg tatccacagt
     3541 acaataatga gcaagaaatc aaagaattac gctttgatga agcatttatg caagaagatt
     3601 tgaatacaga gcgtttgaac gaatttgcag aaaagtttca atcaaaagta ttaagaaagc
     3661 gagttgattt acttttaaaa atccacgatt tatgttgaat ttagaacaaa tacaaaatta
     3721 ttttccgcca caaatcaaag aaaatcctgc acacaaaaaa tatatgctga aagagtacat
     3781 tcagctgatg attttgaatt ttttggccac ttcaaaattt gtgaagaaaa ttacttttat
     3841 aggaggtacg aatttgcgtt tagtgaaagg aatcgacaga ttttcagaag acttggattt
     3901 tgattgtaaa gattttaatg ctgaatattt tgctgagatg tcacaatcgg ttttgacttt
     3961 tttaaaccga tcgggcatac aagcagagag ccgagaaaaa gaaagcaaaa aattaactgc
     4021 ttttcgttca cggatttact ttcccgaatt gttgtttgaa ttagggctgt cggcatacag
     4081 agaagaaaga tttttgatta agttagaaag ccagaatcaa gaatttgaat atacgcccca
     4141 aatgaccacg attagtgcct gtggattttt catgtccttt cctgttccgc ccgatgatgt
     4201 gctttgtgcc atgaaattgt cggcgttact ttcaagagcc aaaggtcgtg atttttatga
     4261 tgctatgttt ttgcttgctc aaacaaaacc aaattatgat tttctcgctc aaaaacaagc
     4321 tattcacgat caagtagaac taaaaacaaa actaatagaa attattgaaa aggtggattt
     4381 agagcataaa tccaaagact ttgaacattt gttatttaac aaagaaaata aaaataaaat
     4441 tgtgaatttc agcgaatttg tgaaaaatct aaattgagga aatgtttctt catttttttc
     4501 ttctgtactt tttttcttga caaaaaagta cccaaaaatc aagactacgc ctgcttcgct
     4561 caaaaaacta gcgttcaaaa agctaaaacc cccaaactcg ccggtttgcg gctcaagcag
     4621 tgggtgtttt ttaacgcttt tttctcttgt tttgcataaa gaactcacgc tcggcatagt
     4681 tcaagcaagc ttgactctgc tctcactcat cgttcttttt tgactcatca gtcgaggtcg
     4741 gtttgcgggg ttgttgaagc agttgccttc gagagcctca ggcaacgaat gttctcggtg
     4801 gctgaggctc tcgaagccac atcatttttt ggctcaccag tcaaggtcgt tctggctaaa
     4861 cattaaacta ttctctgatt cactattcat tattcactat tctttattct tcgactacgt
     4921 acagggtccg ctttataact ttatgagctt tccaacttaa tatccgtaca agttcgcccc
     4981 atctgcgtca tccgtgtgct attattccgc aacacagtgg cttggaagtc agcatcttaa
     5041 gcctgcttat aaaaaccatt acattcaatt ttacataaaa gatgttttaa gtaaaaataa
     5101 ttgtaaattt gcattttaat caaaaatgga aaccaatcgt atagaataca agcaaaaatt
     5161 gactgatcag cttgaaaaag aagttgtggc atttctgaat tatgcaggag gtggaattat
     5221 ctacttagga atagataata tagggaatgt cataggagtt acaggcatag gaactttttt
     5281 ctaaacgaac tcgaaactca attgggaaaa ttaaggctcc gagacaagat ttgaagtttg
     5341 agcaattgag aatttattat gattcaattg acaaaccact taacagtcaa tttgctaaaa
     5401 ctcttgaact actcaatgaa gaagcacaat ataattatgt agcttatttg atggcagata
     5461 ataatactat atctgttaaa cttgcgaaat atagaggtgt aaacagagtc gatttgatac
     5521 aaagtgatga atatggtttt ggatcgctta ttaaagcaat aaaatctgta ttagataaaa
     5581 ttgaagttga aaatacgaca tttactaaaa tcacttctaa agaacgtata gaaaaaagaa
     5641 gatggaattc tttggcaatg cgtgaggctg tgattaatgc tatggcacat aatgattata
     5701 cctacgaatt agctcctaaa tttgagtttt ttgatgatag atttgaaatt acttcttatg
     5761 gtggactacc tcagggacta tcagaaaatg agttttttca aggaatatct attccaagaa
     5821 ataaagagat catgcgtatt ttaagagaca ttgaattggt tgaacaatta ggctcaggaa
     5881 taccgagaat tttgcaatca tatggcaaag aatgctttca gttttctgat aattatatta
     5941 gaataagttt ccctgctgaa tatgatgttg atgaatgtga ggagaaagat acaacaaagc
     6001 atattgaagt gagcgaggat acaaatgtcg gtgtaaatgt cggtgtaaat gtcggtgtaa
     6061 atgaactatt tgaaattata aaaaaatatc ccggaaagaa ctctaaagaa ctacatctgt
     6121 actttgatgt aagcaacaga acaatagaac gttggttaaa aacactaaaa aaagagagaa
     6181 gaattgaatt tattggagca cccaggatag gaggatactt cattgtaaaa tgaatacaaa
     6241 ccaactttac tgacaagttc aacttggaac tcgaaaccca caacccgaaa ctcgcacccc
     6301 gataaaacac cttattttca ccttattttc aaactaaaca agcttagtag aggtgaaagt
     6361 atgaaggtaa aatttacctt attaccttat tttttcttct gcacttttcc ttgataaaat
     6421 acttcttcat ttttttgtct tgatacaaaa aaacgaagca aaaaaaaatc aagactacgt
     6481 tttttcattc cgcacttttt cttgataaaa agtgccaaaa atcaagactg cgcctgcttc
     6541 gctcaaaaaa ttaacgctcg aacaactaaa tcgtccaaac tcgcaacttc gttgctcaaa
     6601 caaggacgat tcttaacgtt gttctcactc attttttggc tcatcagacg aggtcggtct
     6661 gcgggttgtg aatcgctctg caataattca ccaatcgcta ttcactattc gttcttcgtt
     6721 cttcgttctt cgttcttcgt tctttatcgg cgttgtcgga atgacgagca tgatatggtt
     6781 gtacatattc cgccggcaaa tgataacaaa actatcaaca tcccattacg gcgggggtga
     6841 agcatgcaat gcaaaatctg cgtaaatcag cggaactact tgcgaatatc agcgagaaat
     6901 catcaacaat catccgcgag aacctgtaac tcgacaggct gtaatgaacg ggaagcaggc
     6961 aaaaggtaaa aaaacttcat ctactgaaag tttgtacc
//
LOCUS       DAOSIB010000091         6904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_9964_length_6904_cov_11.073733, whole genome shotgun sequence.
ACCESSION   DAOSIB010000091 DAOSIB010000000
VERSION     DAOSIB010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6904
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9964_length_6904_cov_11.073733"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="PLD44_07645"
     CDS             complement(<1..1142)
                     /locus_tag="PLD44_07645"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOF99240.1"
                     /translation="MTQQELKSAFEKIYGQHADAVYFAPGRVNLIGEHTDYNGGYVFP
                     CALSFGTYLLIRKNGGNTVRFRSLNQPDAVEVPLEKLTTRLENSWANYLIGVFAQFIK
                     KGVKIDEGFDILIWGDVPTGAGLSSSASLEVVTAYALNDQLGTGYDRTELALMSQKAE
                     HEFALVNCGIMDQFAAAQGKKDHAIFLNCDTLEFDLVPVKLNGIKVLISNTHSPHKLD
                     SGAYNQRVAECKKAVEQLNKVRSIKYLAELTEEEFKKIESAITDETAKKRARHVVSEV
                     QRTKDAVKALRAGDILQFGKLMNASHVSLRDDYEVTGPELDTMVEEAWKIDGVIGSRM
                     TGGGFGGCTVSLVKDEAIDTFIKNVGAAYEAKIGIKPEFYIAEIGDG"
     gene            1356..1811
                     /gene="smpB"
                     /locus_tag="PLD44_07650"
     CDS             1356..1811
                     /gene="smpB"
                     /locus_tag="PLD44_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HOF99241.1"
                     /translation="MHKTSNIVIKNKRATFEYEILDRYISGIQLFGTEIKSIRSGKAS
                     LSDTYCTFINDELWVKNMHIAPYFFGSYNNHETRRDRKLLLKAQELKRLQRAVKESGN
                     TIVPLKLFINERGLAKLEIALVKGKKLYDKRQTLKEKEDKRYIERTLKR"
     gene            complement(1853..2458)
                     /gene="yihA"
                     /locus_tag="PLD44_07655"
     CDS             complement(1853..2458)
                     /gene="yihA"
                     /locus_tag="PLD44_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOF99242.1"
                     /translation="MEIHAAEFIKSSTDYKGCPEGNFPEFAFIGRSNVGKSSLINMLC
                     QRKNLALTSSKPGKTLLINHFLINKNWYLVDLPGYGFAQTGKENRKKLIDNINNYILF
                     REQLTCLFVLIDVRHEPQKIDLEFMEWLGSNGIPFCIVFTKTDKLSASKIDENIENYK
                     QKMLENWEQLPPFFKTSSHKKIGREEILNFIDKIIESLKVQ"
     gene            complement(2481..3932)
                     /locus_tag="PLD44_07660"
     CDS             complement(2481..3932)
                     /locus_tag="PLD44_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOF99243.1"
                     /translation="MSGFAILLIIAAYFSVLLFISFIVSRRSKDNDAFFLGNRKSPWW
                     VVAIGMVGSSISGVSFVSVPGMVNSIDFTYMQTVFGFFFGYLIIANVLLPVYYKLNLT
                     TIYTYLENRFGKHSYKTGASFFLLSKTIGAAARLYIVALILQNLIFEAWGVPFELTVC
                     GIIILIWLYTHQSGIKTIVWTDTLQTIILITALILIIVEVSQQLNMGVKDIYRELVNY
                     PKTRIFVFDDWNDKQNFFKQFFSGIFIAIVMTGLDQDMMQKNLSCHNLHEAKKNMYWY
                     GFSFVPVNFLFLVLGALLLVFSAEKGIALPPLGDEILPMLAANYLGQTVMIFFITGII
                     AAAFSSADSALTALTTSFSVDILGIQHQESRKANHTRRIIHVCISIFFAIIIVIFKAI
                     NNKSVIDAIYTVASYTYGPLLGMYTFGLFTKMQVKDKFMPYIAVLSPILCGLMDYLFT
                     RYLHYSFGYEILIINGLLTFTGMWIFRKKNIIF"
     gene            4058..4684
                     /gene="recR"
                     /locus_tag="PLD44_07665"
     CDS             4058..4684
                     /gene="recR"
                     /locus_tag="PLD44_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOF99244.1"
                     /translation="MSQQQFSSSLLEDAIHQFAKLPGIGRKTALRLVLHLLKESEEEV
                     ENFGNSIIRLRKEILYCKICHNISDSDICDICSNLSRDRSVVCVVENIKDVITIENTQ
                     QFRGLYHVLGGIISPMDGVGPKDLEIDSLLQRVENDGIKEVILALSTTMEGDTTNFYL
                     YRKLEPFHVKITTLARGVSIGDELEYADEITLGRSIQNRIEFSQTFNK"
     gene            4704..5168
                     /locus_tag="PLD44_07670"
     CDS             4704..5168
                     /locus_tag="PLD44_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99245.1"
                     /translation="MIGKIIKKLLLVYYSVYLAAVVAAVVGYVFIFKNYVGIEAQSSI
                     AINLSSAFILFIILSIPLSLGLFHFQLKKWLLITDKNIRIEKYKKASIYRIIVLGLGF
                     VVGVIFAYLLRSQSIIFCAAMAAAGLIFCKPSEMKMIEELQLDKEENIPAEK"
     gene            5210..5638
                     /locus_tag="PLD44_07675"
     CDS             5210..5638
                     /locus_tag="PLD44_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99246.1"
                     /translation="MIIAVDFDGTIVTHEYPKIGKEIPFAIETLKRLQDEFQHQIILW
                     TVREGRELEEAIEFCKMRGLEFYAINKNYPEEGDDYAESRKIRADLYIDDRNLGGLPD
                     WGVIYQMIAANSVANPFALNGEQPAFRQTKKGFFKSIFGK"
     gene            complement(5686..6720)
                     /locus_tag="PLD44_07680"
     CDS             complement(5686..6720)
                     /locus_tag="PLD44_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOF99247.1"
                     /translation="MKGKNTVLLIFTGGTISMSEDPTTGALRPIDFYRLQEYLPELKQ
                     TGIEIKSIPFSPLMDSSDIQPETWKEIALIIEENFENFDGFVVLHGTDTMAYSASALS
                     FMLQNLSKPVIFTGAQLPIGMMRSDAKENLLTAIEIATAKENGKAIVPEVCIFFEDTL
                     FRGNRTTKKNAEHFNAFNSYNYPPLAKVGVHIKYFNSYIHYPESFSKLEVKTNINPNI
                     AILKLFPGITQQNVHATLNIPELKAVILESYGAGNAPRLIWLYEELKAAVEKGIIIVN
                     KSQCSTGTVEMGKYETSLNLLNAGVISGYDCTTEALVAKLMHLLGEYHSTEIIKHKLS
                     VSLCGEMTVS"
BASE COUNT         2201 a         1200 c         1264 g         2239 t
ORIGIN      
        1 ccatcaccta tttcggcaat ataaaattcg ggtttgattc cgatttttgc ttcataagcg
       61 gctcccacat ttttaataaa agtgtcgatc gcttcatctt ttaccagaga aacggtacat
      121 cctccaaagc ctccgccggt cattcgtgaa cctatcacgc catctatttt ccaagcttct
      181 tctaccatcg tatcgagttc agggccggta acttcgtaat cgtcgcgaag agaaacatga
      241 gaagcattca ttaattttcc aaactggaga atatcgccgg ctctcaaagc ttttaccgca
      301 tctttggtgc gttgaacttc gctcacaacg tggcgagcac gtttttttgc cgtctcgtcg
      361 gtaatcgccg attctatttt tttaaattct tcttccgtca gttcggccag atattttatt
      421 gaacgaacct tattcaattg ctcaactgct tttttacatt cggcaacgcg ttggttataa
      481 gctccggaat ccagcttatg ggggctgtgt gtattggaaa tcagaacttt aataccattt
      541 aattttacgg gaaccaaatc aaattccagt gtgtcgcaat tcaggaaaat ggcatgatct
      601 tttttccctt gcgcagcagc aaactgatcc ataatgccac aatttacaag ggcaaattca
      661 tgttctgcct tttgcgacat cagagccaat tctgtgcggt catatcccgt tcccagttga
      721 tcattcaatg cgtaagccgt aacgacttcc aatgaagcag aagaagacaa tccggcaccg
      781 gttggcacat caccccaaat caatatatca aaaccttcat cgattttgac accttttttg
      841 ataaattgcg caaaaacacc aatcagataa tttgcccacg aattttcgag tcgagtagtt
      901 aatttttcca aaggaacttc tacggcatcc ggctgattca aagaacggaa acgaactgta
      961 tttccgccat ttttacgaat gagcagataa gttccaaaac taagtgcaca agggaaaaca
     1021 taaccgccat tataatcggt atgttcacca attaaattta cccttcccgg agcaaaataa
     1081 actgcatccg catgctgacc ataaattttt tcaaaagctg attttaattc ctgttgagtc
     1141 atattaagta agttttttat agtttctgaa tgcacaaatt taggttaaat cagaatattt
     1201 atctgtttaa aaaatatgtt ttaaaacaga ataatctgat tgttttgtgc atctgatggt
     1261 gattaaaaga attatatgta attttgaatt cccaattttt tcttgtttgt tttagaagtt
     1321 gtaaacaaca attatttgtc aaaaatttta atgcgatgca taaaacatcc aatatagtga
     1381 ttaagaacaa acgtgccact tttgaatacg aaattttaga tcgttatatt agcggtattc
     1441 aattgtttgg aacagaaatt aaatctatta gaagtggcaa ggcaagtcta tccgatacat
     1501 attgcacatt tattaatgat gaattatggg ttaaaaatat gcacatagct ccctattttt
     1561 ttggtagcta caataaccat gaaactcgaa gagaccgtaa attattgttg aaagcgcagg
     1621 aattgaaacg acttcaacgt gcagtaaaag aaagtggtaa tactatcgtt cccttaaaac
     1681 tttttatcaa tgaaagaggt ttggcaaaat tggaaattgc tttggtaaaa ggcaagaagc
     1741 tttatgataa aagacaaaca ttaaaagaaa aggaagataa aagatacata gaaagaactt
     1801 tgaaaaggta atatgttttt ttaacctctt ttctttgtta tgtgcagaga ttttattgca
     1861 ctttcaggct ttcgattatt ttatcgataa aatttaatat ttcttctcta ccgatttttt
     1921 tatgtgagga ggttttaaaa aatggaggca actgttccca attttccaac attttttgct
     1981 tgtaattttc aatattttcg tcgattttcg atgctgaaag tttatcggtt ttagtaaaaa
     2041 cgatgcaaaa aggaattcca tttgatccca accattccat aaattcaaga tctatttttt
     2101 gtggttcatg acgcacatca atcaacacaa acaaacatgt aagttgttca cgaaaaagaa
     2161 tataattatt gatattatct attagttttt ttcggttttc ttttccggtt tgagcaaatc
     2221 cataacctgg caaatctacc aaataccagt ttttgttaat taaaaaatga ttgatcaaca
     2281 gtgttttacc gggtttcgaa gaagtaaggg ccagattttt tcgctgacaa agcatattga
     2341 tgagagatga ttttcccacg ttggaacgcc ctataaatgc aaattcagga aagttccctt
     2401 caggacatcc cttgtagtca gtactgcttt ttataaattc tgccgcatgt atttccattt
     2461 ctatttatga ctttatggtg ttaaaaaatt atgtttttct ttctgaagat ccacatacct
     2521 gtaaaagtaa gtaatccgtt tattatgagt atttcgtatc cgaaagaata atgtaagtaa
     2581 cgcgtaaata aataatccat caatccacat agaataggag ataagacagc aatatatggc
     2641 atgaatttgt cttttacctg catcttggtg aaaagtccaa aagtatacat tcccaacaaa
     2701 ggtccataag tataggaagc tacagtataa atggcatcaa ttacgctttt attgttgata
     2761 gctttgaaaa ttacaataat tattgcaaaa aatatggaaa tacaaacatg aatgatgcgg
     2821 cgtgtgtgat tagcttttcg gctttcttga tgctgaatgc ccaaaatatc taccgaaaag
     2881 gaggtggtta gggctgtgag tgcagaatcg gcactggaaa aggcagcggc aataatacct
     2941 gtaataaaaa aaatcattac tgtttgacca agataatttg cagccaacat aggcagaatt
     3001 tcgtcgccca atggaggcaa ggcaataccc ttttcggcgg aaaaaacaag cagcaatgcg
     3061 cccaaaacaa gaaacagaaa attaaccggt acgaaagaaa atccgtacca gtacatgttt
     3121 ttctttgcct cgtgcaaatt gtgacacgat aagttttttt gcatcatatc ctgatcaagt
     3181 cctgtcataa ctattgcaat gaaaataccg ctaaaaaatt gtttaaagaa attttgcttg
     3241 tcattccaat catcaaaaac aaaaattctt gttttgggat aattaacaag ttctctgtaa
     3301 atatccttca cacccatatt caattgttgg gaaacctcaa caataatgag tatcagtgcc
     3361 gtgattaaaa taatagtttg tagcgtatct gtccatacaa tggttttaat tccgctttga
     3421 tgggtgtaaa gccaaatgag tatgatgatt ccacaaacag taagttcaaa agggactccc
     3481 caagcttcaa aaataagatt ctgtaaaatc agtgccacaa tataaagtcg agcagcagca
     3541 ccaatagttt tcgatagtag aaaaaaagat gctccggttt tgtaagagtg cttgccaaat
     3601 cgattttcaa gataggtata aatggtggta agattgagtt tataatatac aggaagtaat
     3661 acattggcaa taatcagata gccgaagaaa aaaccaaata ctgtctgcat gtaagtaaaa
     3721 tcaatgctgt ttaccatgcc tggtacagaa acaaaactta cacccgaaat agaagaaccc
     3781 accatgccga tggcaacaac ccaccagggc gatttacgat ttcccagaaa aaaagcatcg
     3841 ttatccttgc ttcttctgct tacaataaaa gaaataaaca gtagaaccga aaaatatgcg
     3901 gcaataatca ataaaattgc aaatcctgac atgcttgtta tcatttgaat tttcgagtgg
     3961 caaatttaga caaaaaactt taaagtaact tcccctctta ctcttagttt gttgggaaaa
     4021 aattactaaa tttgcagaaa gtttgtatta tctgtttatg agtcaacaac aattttcctc
     4081 ttcgctcctt gaagatgcta ttcatcagtt tgccaaacta cccggtattg gtagaaaaac
     4141 tgctcttcga ttggtgcttc atctgcttaa ggaatcggaa gaagaagtgg agaattttgg
     4201 aaattccatt attcggctac ggaaggaaat tctgtattgt aaaatttgtc ataatatttc
     4261 cgatagcgat atttgcgata tttgcagtaa tctttcaaga gacagatccg tagtttgtgt
     4321 ggtagaaaat atcaaagatg ttattacgat agaaaatacg cagcaatttc ggggtttata
     4381 tcatgtgctt ggcggaatta tttcaccaat ggatggcgtt gggcctaaag atttggaaat
     4441 cgacagtctt cttcaaagag ttgaaaatga tggcataaaa gaagtgattt tggcattgag
     4501 taccacaatg gaaggcgata cgaccaactt ttatctttat cgtaagcttg aaccctttca
     4561 tgtaaaaatc actacgctgg ctcgtggagt atctatagga gacgagttgg agtatgccga
     4621 tgaaattacg cttggacgct ctattcaaaa ccgtattgaa ttcagtcaga cgtttaataa
     4681 ataaattttt ttgttcagtt cgtatgatag gaaaaataat taaaaaactt ttgcttgtat
     4741 attattctgt ttatctggct gcagttgtgg cagcagtggt gggatatgta tttattttta
     4801 aaaattatgt cggtatagag gctcaaagtt ctatagcaat caatctttcc tcagcattca
     4861 ttttgtttat tattttatcg attcccttaa gtcttggact ttttcatttc caactaaaaa
     4921 agtggttgct tattaccgat aaaaatattc ggatagaaaa atataaaaaa gcttctattt
     4981 atcgaattat tgttttagga ttaggatttg tggtgggagt tatttttgca tatctgcttc
     5041 gttctcaatc cattattttt tgtgcagcta tggctgcagc cggtctgatt ttttgtaaac
     5101 catctgaaat gaaaatgatt gaggaacttc agctcgacaa agaagaaaat attcctgccg
     5161 aaaaatagtt ttcccagaca actaaagttc taaccgaaaa gattttatta tgattattgc
     5221 cgttgatttt gatggaacca tagtaacgca tgaatatccc aaaataggga aagaaatacc
     5281 ttttgccatc gaaaccctga aacgtcttca agatgaattt cagcatcaaa ttattctttg
     5341 gactgttcgc gaaggcagag aactggagga agccatagaa ttttgtaaaa tgagaggatt
     5401 ggaattttat gccattaata aaaattatcc ggaagaagga gacgattatg ccgagtcgcg
     5461 caagatacgt gccgatctat acattgatga cagaaatttg ggaggtttgc cagattgggg
     5521 cgtgatttat caaatgattg ctgccaatag tgtagcgaat ccttttgctt tgaatggaga
     5581 acaacctgct ttcagacaaa ccaaaaaagg tttctttaaa tcgatttttg gaaaataaat
     5641 tttgattgaa gtatattttt ggagtgcttt ataaaatttt cacatttagc ttactgtcat
     5701 ttcaccgcaa agactgacac ttaacttgtg cttgattatt tctgttgaat gatattctcc
     5761 cagtaaatgc atgagtttgg ctaccagagc ttcagttgtg caatcgtaac cacttatgac
     5821 acctgcattc agcaaattta agcttgtttc gtatttgccc atttctacag ttcccgtgct
     5881 gcactgactt ttgtttacaa taataatccc cttttctacg gcagctttca attcttcata
     5941 taaccagata aggcgtggtg cgtttccagc gccataactt tcaagaatga cggcttttaa
     6001 ttcgggaata tttaatgtag catgaacatt ttgctgtgta attccgggaa aaagcttaag
     6061 aatggcaatg ttcggattaa tgttggtttt gacttcgagt ttggaaaaag attcgggata
     6121 gtgaatatag gaattaaaat atttgatatg aacgccaact tttgccaaag ggggataatt
     6181 gtatgaatta aaggcgttaa aatgttcggc gtttttcttc gtggttcgat ttcctctgaa
     6241 aagcgtatct tcaaaaaata tgcagacttc tggcacaatg gctttaccat tttcttttgc
     6301 agttgctatt tcaatggcag tcagcaaatt ttctttggca tcggaacgca tcatgccaat
     6361 tggtaattga gcacctgtga aaatcaccgg ttttgaaaga ttttgtagca taaaactcag
     6421 tgcagaagca gaataagcca ttgtatcggt accatgaagc accacaaagc catcgaaatt
     6481 ttcaaagttc tcctcaatga ttaatgcaat ttctttccat gtttcgggtt gtatatctga
     6541 cgaatccatt aaaggtgaaa aggggatact tttgatttcg attccggttt gtttgagttc
     6601 gggaaggtat tcctgcaggc gataaaaatc tattggacgc aatgctcctg ttgttgggtc
     6661 ttcagacatg ctgatggttc cgccggtgaa tatcaataag acagtgtttt tgcctttcac
     6721 atttgagtat tttttaattt gcgcaaagat aatgcaaatt tcttaacgat tagctttcct
     6781 tttgatattt cttttggcca actgaatttt ttttaaataa ttcattttta gtacatgaca
     6841 aatatttaga catttgtttt atatatctgt aaaacaatac atataagcca agctcttcgg
     6901 gttg
//
LOCUS       DAOSIB010000092         6877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_10020_length_6877_cov_12.158751, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000092 DAOSIB010000000
VERSION     DAOSIB010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10020_length_6877_cov_12.158751"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLD44_07685"
     CDS             <1..304
                     /locus_tag="PLD44_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99248.1"
                     /translation="YELRKQCFDYIDRQNMDYERISAGFCLYGDRGFTELNQAGKKVG
                     TQLNNQYFIYSNISNLPDEWIDELHNPAHWKPLRTFEKWPVQITVFENISFKQTEK"
     gene            301..696
                     /locus_tag="PLD44_07690"
     CDS             301..696
                     /locus_tag="PLD44_07690"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOF99249.1"
                     /translation="MNYPKTLVQFLKFGIVGISNTLLTAVTIWLLLRVFHSSNYLANI
                     VGYVVGLVNSFIWNRRWTFENHAQIGATIFKFIVTFAVSYLLQLGFLWLLLHQTEIDD
                     YFCQLLSIVVYTVVNFFMNKYYTFKTEIK"
     gene            693..1682
                     /locus_tag="PLD44_07695"
     CDS             693..1682
                     /locus_tag="PLD44_07695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOF99250.1"
                     /translation="MNSSLSVIVPCFNEEEVISESYHRLKKVLQELPLASQIIFVNDG
                     SIDGTEKILSEIAQADKTVKVISFSRNFGHQNAVTAGLNHCLSDLAVVIDADLQDPPE
                     LIPAMIETLEKNNAQVVYCVRKHRKGEGFFKKISAKLFYRFLNFFSEVRLPVDTGDFR
                     LVDKNVIREFNKLHEKGKYIRGLIVWLGFKQVPFYYEREPRFAGETKYPFSKMMKLAT
                     TSLLYFSKKPLKIATYLGFFTVIIALLLALWAIFGKFFGFTNADPGWTSLMVAVVFFG
                     GVQLLTVGILGAYIGNLFDEIKDRPEYVIKDMMNFETGNSTQIADEQTGLQNG"
     gene            1692..2858
                     /locus_tag="PLD44_07700"
     CDS             1692..2858
                     /locus_tag="PLD44_07700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOF99251.1"
                     /translation="MIKTLFISAWYPNRYDEMFGLFVRKHAEAVSLYCDVQVLYVHPS
                     SDIRHFETEIRQNGKVKETIVYFPTSKSGVFSKLLKQINFLRAYLKGWRMLQKSGFRP
                     DIVHANILTRTVVMAYGIHLFTGIPYVITEHWTRYLPLRKSYNGLLRKKITEFVVKHA
                     AAVFPVSENLKNAMISNKLWNKNYFVVNNAVDDVFLTDISKVSETKKIILHVSCFDEQ
                     QKNVKGLLNSLKVLSVNRSDFKMIFIGTGVDFKEVVDYSVKLNFPEDVIHFVGEKNPA
                     EVAEYMKNADFFVLFSNYENSPVVISESLVCGKPVISTDVGGIAEHINETNGILIPAR
                     DENMLFESLNFMLDHYQDYDSEKLSQEAVEKFSMKNVGKTIAQQYEKILGKNPE"
     gene            2962..4671
                     /locus_tag="PLD44_07705"
     CDS             2962..4671
                     /locus_tag="PLD44_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOF99252.1"
                     /translation="MHGFNVLISKKAIKQQPHFCWKMPFDFQQNLALRTFSDEHIVVE
                     QFTGTKFISEKLWLDNEDFFIVTDGVITNLKALCLEHNAANYDALLRKIVKEKSFFKN
                     FRGHFVGFVYFKKEKTCIAFNNHTASKKLFYFADQNTIVIGTDLYTLSKTLETEKIKR
                     TLDKEAAVFLLTSGFMHENLTLLEEVKQLRAGEFLQIGNFNLKTDFYFHLQDIQENHD
                     AKSDIVEQLDFLFKKSIDNEFSLDKEHQLISLTTLSGGLDSRMVALTALQLGFSNQMF
                     LNFSAKGYADEIIARQISEQYQVPLETFSIEAESLKPLEEVVLVNDGLTIYTGASAVF
                     DAIKRHRITNTGIIHTGMLGDALLGSYLISDHKTKPQISDGVYSTQLLNKYAPVFQKY
                     IQNYSDIEMYKFYNRGFSGMNNGFLYFDLCGESDAAFLNPDFLSYALSIPAKYRYKEQ
                     IYIDWIKIKHPEFTGFTWESIGGKPTNNSILRCYYRYRRALVKRMPLQTMWKNNMNPE
                     QLWYDKNLSLRQTLDQYFLENIHLLSSCSELFHDCQFLYQKGNITEKAQVLTLLAACK
                     LLL"
     gene            5313..6533
                     /locus_tag="PLD44_07710"
     CDS             5313..6533
                     /locus_tag="PLD44_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase-maturation protein"
                     /protein_id="HOF99253.1"
                     /translation="MDILFNPLSMPTYLMAKPISSICNLNCSYCYYLEKEKLYKNYKS
                     FEMSDLTLEKYIESYIQAQPVPDVLFTWHGGEPLLRGISFYRKALSLQKKYGRGRRID
                     NSLQTNGILLNDEWCRFFKDNNFLIGISIDGPEHCHDYYRKNKGGKGTFRQVMHGIEL
                     LQKHQVEFNTLSVIHNYNVDFPLEIYHFFKEIGSQFMQFSPIVERLSISREDGLTLLP
                     LQGSADAKLAPWSVDAVKFGEFYIQIFDEWVKKDVGRYFVQLFDATLANMVGEQPGTC
                     IFAETCGHAMVMEFNGDVFYCDHFVFPEYKLGNIHTHTVFEMAFSEKQLQFGKNKRDL
                     LPSQCKKCDFLKLCKGECPKNRILRTPIGENGLNYLCQGYKNFFRHSSPYMEFMANEL
                     FFHRSPANVMNWKK"
BASE COUNT         2182 a         1093 c         1345 g         2257 t
ORIGIN      
        1 ttacgaatta agaaagcagt gttttgatta catcgaccgg caaaacatgg attatgagcg
       61 catttcggcg ggtttttgtc tgtatggcga cagaggcttt acagaactga atcaggcagg
      121 gaaaaaagtc ggtacacagc tcaacaatca gtattttatt tattccaata tttccaattt
      181 gcccgatgaa tggatagatg aattgcataa tccggctcat tggaaacctt tacgtacttt
      241 tgaaaaatgg ccggttcaaa ttactgtttt tgaaaatatt tctttcaaac aaacagaaaa
      301 atgaattatc ccaaaacatt ggttcagttt ctgaaattcg gaatagtcgg aatttccaat
      361 acgttgctta ccgcagtaac gatatggctg ttgttgcggg ttttccattc ttccaattat
      421 ttggccaata ttgttggtta cgtggttggg ttggtaaaca gctttatttg gaatcgacgc
      481 tggacttttg aaaatcatgc gcaaattggc gcaaccattt tcaaatttat tgttacattt
      541 gctgtttcct atttgcttca attgggattt ttatggcttt tgctgcatca aaccgaaatc
      601 gatgattatt tttgtcaatt attgtcgatt gtggtttata cggttgtcaa ttttttcatg
      661 aacaaatatt atacttttaa aaccgaaata aaatgaattc atccttgagt gttattgttc
      721 cgtgtttcaa cgaggaagag gtaataagcg aatcatatca tcggttgaaa aaggttttac
      781 aggaactgcc tctggcttca caaataattt ttgtgaatga tggaagtata gatggaacgg
      841 aaaaaatttt atcggaaatt gcccaagccg acaaaacggt taaagtcatc agtttttcca
      901 gaaatttcgg tcatcaaaat gccgttacgg caggtttaaa tcattgtttg agcgatttgg
      961 ccgtagttat tgatgccgat ttgcaggatc ctcccgaatt gattccggcc atgattgaga
     1021 cgctggagaa aaataatgcc caagtggttt attgtgtaag aaaacatcga aagggagaag
     1081 gtttttttaa aaaaatctct gcaaaattgt tctatcgttt tctgaacttt ttttccgaag
     1141 tcagacttcc ggtcgatacg ggcgatttca gactggttga caaaaatgtg atcagagaat
     1201 tcaataagtt gcacgaaaaa ggaaaatata ttcgcggact gatcgtttgg ttgggcttta
     1261 aacaggtgcc gttttactac gagcgcgaac cccgttttgc gggtgaaacc aaatatcctt
     1321 tttccaaaat gatgaagctg gcaactactt ccttgcttta tttttctaaa aagcctttga
     1381 aaattgcaac ctatttgggc tttttcacgg taattattgc tttgctgctg gcattatggg
     1441 caattttcgg aaaattcttc ggtttcacca atgccgatcc gggttggacg tcactgatgg
     1501 ttgcggtggt attttttgga ggtgttcaat tactgacggt cgggattctt ggcgcttata
     1561 tcggtaattt gtttgacgaa attaaagatc ggcctgaata tgttatcaag gatatgatga
     1621 attttgagac aggaaacagt acacaaatcg ctgatgaaca aacaggttta caaaacggat
     1681 aatttgaaaa aatgataaaa acgcttttca tatcggcatg gtatcccaat cgatacgacg
     1741 aaatgtttgg gctatttgtt agaaaacatg ccgaagctgt gagtttgtat tgtgatgttc
     1801 aagtacttta tgtgcatcct tcgtccgata ttcggcattt tgaaaccgaa attcgccaga
     1861 atggaaaagt gaaagaaacc attgtttatt ttccgacttc caaatcaggc gtgtttagca
     1921 aacttttaaa acagataaat tttttacggg catatctcaa aggttggcgt atgttgcaaa
     1981 aatccggttt ccgtcccgac attgttcatg ccaatatact tacgcgtacc gtcgtgatgg
     2041 cttacggaat tcatttgttt accggcattc cctatgtaat tacggagcat tggacgaggt
     2101 atttaccctt aagaaaaagt tataacggat tattgagaaa aaaaattacc gaatttgtgg
     2161 tcaaacatgc tgctgcagtt ttccctgtgt cggaaaatct gaaaaatgca atgatttcca
     2221 acaagttatg gaataaaaat tattttgttg taaacaatgc ggttgatgat gttttcttga
     2281 cagatatttc caaagtatcg gaaacaaaaa aaattattct tcatgtttcc tgttttgatg
     2341 aacagcaaaa aaatgtaaaa ggtttgttga attcgttgaa agttttgtct gtaaatcgaa
     2401 gtgatttcaa aatgattttt atcggaacag gcgttgattt taaggaagtg gtagattatt
     2461 ccgtcaaatt aaattttccg gaagacgtga ttcattttgt gggagaaaaa aatcctgctg
     2521 aagttgccga atacatgaaa aatgcggatt tttttgtatt gttttcaaat tacgaaaatt
     2581 cccctgttgt tatttctgaa agtttggttt gcggtaagcc cgttatcagt acggatgttg
     2641 gaggaattgc cgagcatatt aatgaaacaa acgggatatt gattcccgca agggacgaaa
     2701 atatgctttt tgaaagtttg aatttcatgc ttgatcatta tcaggattac gactccgaaa
     2761 aattgagcca ggaagctgtc gaaaaattca gcatgaaaaa cgtaggaaaa acgattgctc
     2821 aacagtacga aaaaatttta ggaaaaaatc ccgaataata aattcttcaa tcaacaagtt
     2881 ttgttgcctg attaagtttt ttcattgtaa attcatttta ttgccaacgc agttcgtaac
     2941 aattatttca gaaacggttc tatgcacgga tttaatgttc ttatttcaaa aaaagccata
     3001 aagcagcaac ctcatttctg ttggaagatg ccttttgact ttcagcaaaa ccttgcgctt
     3061 cgaacttttt ctgacgaaca tattgtggtg gaacaattta cagggacgaa gtttatttcg
     3121 gaaaagttgt ggctggacaa tgaagatttt tttattgtta ccgatggcgt cattaccaac
     3181 ctgaaagccc tttgcttgga acataatgca gcaaattatg atgcgttgct caggaaaata
     3241 gttaaagaaa agtctttttt taaaaatttt cggggacatt ttgtcggatt tgtatatttc
     3301 aaaaaagaga aaacatgtat cgctttcaat aatcacacgg catcgaaaaa acttttttat
     3361 tttgccgatc aaaataccat tgtgataggt accgatttgt acacactgag caaaactctc
     3421 gaaaccgaaa agataaaaag aactttagac aaggaagccg ccgttttttt actcacaagt
     3481 ggatttatgc acgaaaactt aaccttgttg gaagaagtga agcaattgcg ggccggagaa
     3541 tttttgcaaa taggcaattt caacttaaag acagattttt attttcatct tcaggatatt
     3601 caagaaaatc acgatgcaaa atccgatatt gtcgaacaac tcgattttct ttttaagaag
     3661 tcgattgata acgaattttc tcttgacaag gaacatcaat tgatttctct gactactctg
     3721 agcggaggcc tcgattcgcg aatggtggca ctcacggctt tgcagttggg cttttcaaac
     3781 cagatgtttt taaatttttc tgcaaagggc tatgctgatg aaataattgc gcggcaaatt
     3841 tctgaacaat atcaagttcc gttggaaact ttttctattg aagccgaaag tctgaaacct
     3901 cttgaagagg tcgtgctggt gaacgatgga ctaaccattt ataccggagc aagtgcagtg
     3961 tttgatgcca taaaacggca cagaattacc aatacgggca taattcatac cggtatgctg
     4021 ggagatgctc tgttgggaag ttatctgatt tcggatcata aaaccaaacc acaaatttct
     4081 gatggtgtct attctaccca attattgaat aaatatgctc cagtttttca gaaatacatc
     4141 caaaattatt ctgacattga aatgtataaa ttttacaaca gaggtttttc cggcatgaat
     4201 aacggttttt tgtatttcga tttgtgtggc gaatcggatg ctgctttttt aaatcccgat
     4261 tttttgtctt atgctctttc aatacccgca aaataccgat ataaagaaca aatctatatt
     4321 gattggataa aaatcaaaca tcccgaattt accggtttta catgggaatc cataggtgga
     4381 aaaccgacca acaacagtat attgcggtgt tattatcgct atcgtcgtgc tttggtgaaa
     4441 cgtatgcctc ttcaaaccat gtggaaaaac aacatgaatc ccgagcagct ttggtacgat
     4501 aaaaacttgt cgcttcggca aacgctggat caatattttt tggaaaatat tcatttgctc
     4561 tcatcctgta gcgagttatt ccacgattgt caattccttt atcagaaagg aaacattacg
     4621 gagaaagcgc aagtgcttac tttactggct gcctgtaaat tgttgttgta gaataaataa
     4681 atgagtcccc tccgaaaagt tactcaaaga gaattagttt ttttaagaca ttgagataaa
     4741 aaaggataca aaatattcaa aaaatctttg aaaaatttgc atatattgtt atattcacta
     4801 tcttagcagg gttttggtgg ggatgatgat tttaaaagaa ggtcagggct ggagcgacga
     4861 gcagttaatt gagccatcat aaaaaatgcg gtaaaaaaca gagaatggca actatcgtta
     4921 ttttaatctt gagcaggggc gaacttctgt tttgagacaa aaactacgaa acattccaaa
     4981 agaaaaactt gaccatagaa aatttttcag ttaggttacc attattccaa tgaaaagaca
     5041 cgttatcgcg gacttgccaa acatcgctta tgggcttatt ctcgcacttt gtggataaac
     5101 ttcgctcgaa tactggaata tatagtgcaa atcgctcaaa gcacgcttaa tgcattacaa
     5161 aaagctcctt ttgtaacaaa aataactttt atattgcaaa ttattgtaaa acatgaaaca
     5221 acaaaaaatt taatacttct cttcaaaaaa atcttatttt ttctcttttt tgaaaaaatc
     5281 aagttttagg agagggctca aaatttaatt ttatggatat tttatttaat ccactttcaa
     5341 tgcctactta tttaatggca aaaccgatca gttccatttg taatttgaat tgtagttatt
     5401 gctattatct ggagaaagag aaactataca aaaattataa atcatttgaa atgagtgatc
     5461 taacactcga aaaatacatc gagagttata ttcaagcaca gcctgtcccg gatgtacttt
     5521 tcacatggca tggcggagaa ccattattaa gaggcatttc tttttatcgc aaagcgcttt
     5581 ccttgcaaaa aaagtatggt agaggcagaa ggattgataa cagtttacaa acgaatggaa
     5641 ttttattaaa tgatgaatgg tgtcgttttt ttaaagacaa taattttttg attggtatct
     5701 cgatagatgg accagaacat tgccatgact attatcggaa aaacaaagga ggaaaaggaa
     5761 ctttcaggca agtgatgcat ggtattgagt tgttgcaaaa acatcaggtg gagttcaaca
     5821 cactttctgt cattcataat tataatgttg attttccatt ggaaatttat cattttttca
     5881 aagagattgg gagccagttt atgcaatttt cgcctattgt ggagcgacta tcaataagta
     5941 gagaggatgg attgacttta ttgccactcc aaggaagtgc ggatgccaaa ttagcaccat
     6001 ggagtgtaga tgcagtaaaa tttggagaat tttacattca aatttttgat gaatgggtaa
     6061 aaaaggatgt aggtagatat tttgttcaac tttttgatgc aacgttggcc aatatggttg
     6121 gcgagcaacc tggtacttgc atttttgcgg aaacatgcgg tcatgctatg gtcatggaat
     6181 ttaatgggga tgttttttac tgtgaccatt ttgtttttcc ggaatacaag ctggggaata
     6241 ttcataccca tactgttttt gaaatggcat tttctgaaaa acaacttcaa tttgggaaaa
     6301 ataaacgcga tttgctccct tctcaatgca aaaaatgtga ctttttaaaa ctttgtaaag
     6361 gcgaatgtcc aaagaacaga attttgagaa ccccaattgg cgaaaatgga ttaaattatc
     6421 tttgccaggg gtataaaaac ttttttcgac acagtagtcc atatatggaa tttatggcta
     6481 atgaactgtt ttttcatcgt tcacctgcta atgtaatgaa ttggaagaaa tgattttcta
     6541 ttcttattga aaagatggtt ttaatagctc ttgccttaga cattccaaag ttcaattgta
     6601 aggatgaatc ataaattatt cgaaattgtt actaatgctc ttactttcaa aatatttttg
     6661 taaataatta atattaaatg tattacaagt attctttggt tttatgactt gattggggga
     6721 aaataataat aattttatga tttttgttta gctaatttca aagagataga aaaatttatt
     6781 ttgctgtttt cttgtctagt tttatcaatt aattgtttgg tttcactgcc gtacgaaagt
     6841 ttttaaaaat gttgttcttg aaaattcata tatttgt
//
LOCUS       DAOSIB010000093         6870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_10029_length_6870_cov_13.783272, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000093 DAOSIB010000000
VERSION     DAOSIB010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6870
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10029_length_6870_cov_13.783272"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..334)
                     /locus_tag="PLD44_07715"
     tRNA            complement(260..334)
                     /locus_tag="PLD44_07715"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(297..299),aa:Val,seq:tac)
     gene            complement(531..2888)
                     /locus_tag="PLD44_07720"
     CDS             complement(531..2888)
                     /locus_tag="PLD44_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOF99254.1"
                     /translation="MSRIKLRTWLYYVLMLLLFCVGAYLLIQKGSDVEANYHLTTQSV
                     STQVLSPEPNLSNWEQFKVSVSHNMGEPITHLLLQIIAILLVSRLFGLFFSKIDQPIV
                     IAEILAGIVLGPSLLGHFFPNIFNFLFAADSLPNLYILSQIGLILFMFVIGMELNMKA
                     LKEKVSQVLVISHASILIPYFLGMSLAYVYYEEFAVNQTKFLPFALFIGISMSITAFP
                     VLARIVQEKGLTKTHLGTIAIASAAIDDVTAWCLLALVIAISKTGDSVSALYTVGLSV
                     AYILLMFFVIRPFLKKIGEIYQNIETLNKSIVAFLFLVLISSAFITQFIGIHALFGAF
                     LAGVVMPPISNFRKLVIEKVEDVSLTLLLPLFFVFTGLRTEIGLLNSPYLWGICGIFI
                     ATAIVGKFVGGAFSARFLGESWHDSLALGTLMNTRGLMELIVLNIGYEMHILPPSIFV
                     MLVIMAIVTTFMTTPILNLIEKVFPPKNVEEEYIRKQSMGIFKVLIAVGNPENGRMLL
                     RVAKNVLEGVKNTLSVTVLHITPGTDTNPIYGDQFAEESFHKVKQEAEQLNIPIETEY
                     KVVDSIENAIVRMANTNNFDFLLVGAGLSLSGIPFFKERAVFKNIGWLNRLINNISKQ
                     QVVFYPGTLIKDKTKYFIEQSNCTVGVFVNRGFSEISSAAVLLYSEQDEFLLHYARRL
                     LRNNNNASVTIFDLNRVVASSEKVARYVEDLEKQFPASVKLNKQNKINSNSFSKFSLM
                     FISYQTWNALLEKDKSYLSNIPSTLIINKKNSRFHSDGRRRQVLS"
     gene            3711..5111
                     /locus_tag="PLD44_07725"
     CDS             3711..5111
                     /locus_tag="PLD44_07725"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOF99255.1"
                     /translation="MNKRKLLYRYLISDTLASLVVWVVFMIFRKIVNDAAIFENVQIF
                     FPNYNYLSSLILFPLSCITVHYLSGFYLYPEKHSGIKIFFTTFVASLIISIVIFFVLM
                     LDDIVVSYKYYYYSLAVLFGLLFVFTYLPRLIIYSNVKSNYRSKKWTIPTVIIGTGQN
                     AIKIGNELAKAAPEKTVVGYVETNYGKQISQEMILGNLHQIEKIIEKYNIQEAIIALD
                     QTDDYQLFSIINSLYRFNIDIQFTPRLYEILTGSARIDNLSISPLVSITDIKMPDWEQ
                     SVKRTFDIGFSLLALVLLSPVLLYFMIRIKTDSKGPVFYLQERIGRYGRPFKIIKFRT
                     MFEGAENGIPRLSNPNDNRITNIGKKLRKYRIDEIPQFINVLLGHMSIVGPRPERPYY
                     IQQIVQEAPYYCLLYKIRPGLTSWGPIRIGYSDSIEKMIERLNYDIIYLESMSLITDL
                     KILLLTLEIIFKGKGV"
     gene            complement(5203..6471)
                     /locus_tag="PLD44_07730"
     CDS             complement(5203..6471)
                     /locus_tag="PLD44_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOF99256.1"
                     /translation="MKFFLRLNKLLSEISAKGFFHLLSANVLIQLVAFASQLFVAGIL
                     LPEDVGRIKIITTFLSVFSIIGGMGLHASTLKLCSENRSQKEISELFSSGVVFTIVST
                     IVFYGLALVLNSFGFFSSDAIIRRLIPLGLFPLITNSAFMVLVAYFQASKQIKIMSRI
                     TVANKLIAIVAIVLFTWWFGINGYYVAYNLSFIVMFFVGFRLLRKDFTFSKDRESVKH
                     NFKTHLVYAKPSLMSNLLSEISAYADIFVINYLLKDMKEIGYYSFALTLTVVLRVFPF
                     TVQQIASPYFSGLAHDRSHYLQVFKKYNRQLYLVVGVTLLLAILAGPFLIRIIFAGKY
                     DPAIPYFVLLSVGWSIRLLNQLQSAAIFGLGKIQYNAWSSFISLIFNVAVYIVFVRLY
                     GVIGAAWASIPAGVVMTLTSGLFLRKAMRE"
BASE COUNT         2352 a         1302 c         1236 g         1980 t
ORIGIN      
        1 acaagaagaa tttatgcggg gcagcaaaag ataatttttt aattttttca aaattaatca
       61 agttttcatt cgggtgctct tttttctcat tgtttcgttt cgctacgctt cacttctcaa
      121 tgagaaaaaa gcatcaacaa cgtggtgaaa ctttacagct aagcccctat ttgtgcagat
      181 gatccgatga gccgagatgg aaattcgtaa aatacaagca ataaaaaaaa gagctacatt
      241 tctgtaactc ttagttttgt gggcgttgac ggattcgaac cgccgaccct ctgcttgtaa
      301 ggcagatgct ctgaaccagc tgagctaaac gcccttgttt gtttatgtac ctgctttcgg
      361 taaaagcgtt gcaaagatac attgtttttt caaattgaca aaaaatttgt ttaatttttc
      421 gcatataaaa tttttagatg ctgtaaattg gttcttatta ataatctcat catttaaaat
      481 cctgaaaaaa ttaataaaac tgtctattca tctacattct cttctgaaat ttaagataat
      541 acctggcgcc gtctgccatc agaatgaaaa cggctgtttt tcttgttgat aataagtgtc
      601 gaaggtatat tcgacagata cgatttatcc ttttccaaca atgcgttcca tgtctgatag
      661 ctgataaaca tcagactaaa tttggagaaa ctgttggaat tgattttatt ttgcttgttg
      721 agcttgacag aagcggggaa ttgcttttcc aaatcttcaa cataccgggc tactttctcg
      781 ctggaagcca ccactctgtt caaatcgaaa atggtaaccg aggcattgtt gttgttgcgc
      841 aagagacgtc gtgcataatg tagcagaaac tcgtcttgct cactgtagag caaaacagca
      901 gctgagctaa tttccgaaaa tccacgatta acaaacactc ccaccgtaca attactctgt
      961 tctatgaaat acttggtctt gtccttaatc aaagttcccg gataaaacac cacctgttgt
     1021 tttgaaatat tattgatcag tctgttcagc catcctatat ttttaaaaac agctctttcc
     1081 ttaaagaatg gaatacccga caaggaaagt ccggctccca ccagaaggaa atcgaaattg
     1141 ttggtatttg ccattcgtac aatggcattt tctatgctgt caaccacttt atattccgtt
     1201 tcgataggga tattcagttg ctcagcttcc tgttttacct tatggaaact ttcctctgca
     1261 aactgatctc cataaatggg attggtatcg gtgccgggcg taatatgaag tacagtaacg
     1321 gaaagagtat tttttactcc ttccaaaaca tttttggcaa cacgtaacaa cattctccca
     1381 ttttccggat ttcctacagc aatcaacacc ttgaaaattc ccattgactg cttgcgaatg
     1441 tattcttctt ccacattctt tggagggaaa actttctcta tcaaattcag aatcggagtg
     1501 gtcataaagg ttgttactat ggccatgatt accagcatta caaaaatgga aggaggaaga
     1561 atgtgcattt cataaccaat attcaacaca atcaattcca tcaatccacg cgtattcata
     1621 agtgtcccca atgccagact gtcgtgccac gactcgccca gaaaacgtgc ggaaaaagcc
     1681 cctccgacaa atttaccaac aatggctgta gcaataaaaa ttccgcaaat tccccataaa
     1741 tagggagaat tgagcaaccc tatttcggta cgcaaaccgg tgaatacaaa aaacagaggc
     1801 aaaagcaaag tcaacgaaac atcttcaact ttttcgatca ccaattttct gaaattggaa
     1861 atgggaggca ttaccacgcc ggccagaaaa gcaccgaaca aagcatggat accgataaat
     1921 tgtgtaataa aggcagaaga aatcaaaacc aggaaaagaa aagcaacaat acttttattg
     1981 agggtttcaa tattttgata aatttcgcca atttttttca aaaaaggtct gataacgaaa
     2041 aacataagta aaatatacgc aacagacaat ccaaccgtat aaagtgcgct aacggaatca
     2101 ccggtttttg aaatggcaat gaccagtgcc agtaaacacc atgctgtaac atcatcaatg
     2161 gctgcactgg ctatggcaat tgttcccaaa tgtgtcttgg taagaccctt ttcctgaaca
     2221 atacgcgcca aaacggggaa ggcggtaata ctcatggaaa ttcctataaa cagcgcaaaa
     2281 ggaagaaatt tagtctgatt aacggcaaac tcttcataat aaacataggc caacgacatt
     2341 cccaaaaaat aaggaatgag aatactggca tgactgataa ccagcacttg acttaccttt
     2401 tctttcaacg ctttcatatt caactccata ccgataacaa acataaaaag aatcaaccct
     2461 atctgactta gaatatacaa attgggtaaa gaatcggcag caaagagaaa attgaaaata
     2521 ttgggaaaga aatgccccaa caaagaaggt ccgagcacaa tgccggctaa gatttctgct
     2581 attacaattg gttgatctat cttggaaaaa aatagtccaa aaagtcttga aaccaaaagt
     2641 atggcaatga tttgaagcaa taaatgggtg atgggctcgc ccatattgtg ggacacagaa
     2701 actttaaact gttcccagtt gctgagattg ggttccggag aaagaacttg ggtagaaaca
     2761 ctttgtgtgg ttagatgata atttgcctcc acgtcgctcc ctttttgtat caacaaataa
     2821 gctccaacac aaaaaagaag taacatgagt acataataaa gccatgttcg caatttgatt
     2881 ctggacatag aatttacttt atcatgctat gcagtgaaaa ttcatttttt aaattctctg
     2941 acagcctccc acagttagga aaagtaagga aatccgctac aaaaatacaa aaaattaaaa
     3001 aaacaatgca aaactttctg ctaaatcagc acctttatgt tgaacttagc atagtgttag
     3061 ctatcgtaaa agataaatcg gttgtatggc gtgaactata tgtattacat taaattattg
     3121 tacaaataaa ttattatctt tgtccttagt agatgataaa aaaaattgta aaaacattta
     3181 tgttttttat tttcagatat ttaataacga agctttgccc caaacctcac ttactttttt
     3241 gtcttgaaac aaaaacgtaa gcaaaaaaat taacttagtt gaagcagcta cgctcaacat
     3301 aaatcaaata aatttgattt tgttttcgct taaacgctgc tttcaagact gtgtccactt
     3361 cactcgaaaa acttacgctc gaaaagctga aatcgcccaa actcattccg acgaaatacg
     3421 tcggaactca aacaaggacg atttttaaac gcttttctca cttgtttttc ggctcaccgg
     3481 acaaggtcaa cccaagtgct tggctgatat gtcgtgtttt acagatatat aaaacaaata
     3541 tttcaatttt tgccatgtat taaagtaata attataaagc ggtgaaaaga aaataaagaa
     3601 tactaatttt cgtttataaa aaattgagag atgataagtg tcgcaaaata ttgaaaacaa
     3661 ttataaagta atagaattca aaatttagaa atttcctcat cagcgagcgg atgaacaaga
     3721 gaaaactgtt atatcgttat ttgatttcgg atacactggc ttcactggtg gtgtgggtag
     3781 tgttcatgat tttccgaaaa atagtgaatg atgcagctat ttttgaaaat gttcaaattt
     3841 tctttccgaa ttacaattat ctttccagtc tgatactttt tccgttgagt tgtattacag
     3901 ttcattatct ttccggattt tatctttatc ctgaaaaaca ttcaggcata aaaatatttt
     3961 ttaccacttt tgtagcttca ttgattattt ccattgtcat tttttttgtg ttgatgctcg
     4021 acgatattgt ggtatcttac aaatactact attattctct cgccgtgttg ttcggattgt
     4081 tgtttgtgtt tacttatttg cctcgtctta ttatttattc caatgttaaa agcaactatc
     4141 gttcaaaaaa atggactatt ccgacggtta ttattggaac gggacaaaat gccatcaaaa
     4201 tagggaatga gctggcaaag gcagctcctg aaaaaactgt ggtaggatat gtggaaacga
     4261 attatggaaa gcagatatca caggaaatga tacttggcaa tttacatcaa atagaaaaaa
     4321 ttatcgaaaa atacaacata caggaagcca ttattgcact cgatcaaact gatgattatc
     4381 agcttttttc aataatcaat tccctctatc gcttcaatat cgatattcag tttacgccac
     4441 gactgtatga aattttaacc ggaagtgccc gcatcgacaa tttaagcatt agtccgttgg
     4501 taagtattac cgacataaaa atgcccgatt gggaacaaag cgtcaaacgg acattcgaca
     4561 ttggcttttc gctgttggct ttggtcttgc tttcgcccgt tttgctgtat tttatgattc
     4621 ggattaaaac ggattccaaa ggccctgttt tttatcttca agaaagaatc ggacgttatg
     4681 gtcggccatt caaaattatt aagttccgaa ccatgtttga aggtgcggag aacggcattc
     4741 cccgattgag caatcccaac gataatcgga taacgaatat tgggaaaaag ttgagaaagt
     4801 atcgcattga cgaaattcca caatttatca acgttttatt gggacacatg agcattgttg
     4861 gtccgcgtcc cgaaaggcca tactatattc agcaaatagt tcaggaagct ccctattact
     4921 gtctgttata caaaattcgg ccgggactca cctcgtgggg acctatccgc attggctatt
     4981 ccgacagtat cgaaaaaatg atcgaacggc tgaattacga cattatctac ttggaaagca
     5041 tgtcattgat taccgacctg aaaattctcc ttcttacttt ggaaattatt tttaagggaa
     5101 aaggagtgta aacttgtaca aaaatttgaa taaaagaacc aaaatttgtc aaagtttttc
     5161 gcctagtcga tgtaaatcaa taaacaaaaa ataccgaacc agttattccc gcattgcttt
     5221 tctgagaaac aaacccgatg tgagggtcat gacaactcca gccggaatac ttgcccatgc
     5281 cgctccaata acgccataca agcgaacaaa aacaatataa acggctacgt tgaatatcaa
     5341 cgaaataaaa ctgctccaag cgttgtactg aatttttccc aatccaaaaa tggctgcact
     5401 ctgaagttgg ttcagcaagc ggatactcca acctaccgac aacaggacga aataaggaat
     5461 ggccggatcg tattttcccg caaaaataat cctgataaga aaaggccctg ccaatatggc
     5521 cagcagcaat gtaacgccaa caaccaaata aagctgtcgg ttgtattttt taaaaacctg
     5581 caaataatgg ctgcggtcgt gggcaagtcc tgaaaaatag ggtgatgcta tttgttgcac
     5641 cgtaaaagga aaaacacgca gcactacagt caacgtcaat gcaaaactgt agtaaccaat
     5701 ttctttcatg tctttcaaca aatagttgat cacaaaaata tcggcataag cagaaatttc
     5761 ggaaagcaga ttggacatca atgaaggttt tgcataaact aaatgtgttt taaaattatg
     5821 tttgacactt tccctgtctt tggaaaaagt aaaatctttt cgcaacaacc ggaacccaac
     5881 aaaaaacata acaataaaac tcaggttgta agccacataa tagccattaa ttccaaacca
     5941 ccacgtgaac aacacaatgg caacaatggc aatcagttta ttggcaacag ttatgcggga
     6001 catgattttg atttgtttgc tggcctgaaa ataagcaacc aaaaccatga aagccgaatt
     6061 cgtaatcaga gggaaaagtc ccagcggaat aagcctgcgg atgatggcat cggaagaaaa
     6121 aaatccaaaa gaattcaata caagtgccaa cccgtaaaaa acaatggtgg aaacaatggt
     6181 aaacactaca ccggaactga aaagttcact gatttctttt tgtgaacggt tttcggaaca
     6241 aagtttcaag gtagaagcat gcaaacccat tcctccgatg atggaaaaaa cagaaagaaa
     6301 agtggtgata atttttattc gtccaacgtc ttcgggcaaa aggataccgg ccacaaaaag
     6361 ctgcgaagcg aaagccacca gttgaatcaa aacattggcc gacagcaaat ggaaaaagcc
     6421 ttttgcagaa atttcggata aaagtttatt gagccggaga aaaaacttca tgtttttcat
     6481 cttttgccta tattctttct gacacataca tttatttact aaagtttcgc atttcagaaa
     6541 gggctgaaaa taagagagaa aaaaaagtaa aaaagtagca tattttattt aatatctcat
     6601 taaaaatgag tatgttagct gtataattac aaaacagaaa aaatatgcta ccggacaaaa
     6661 ttaaggaaaa aatttctgaa acaggaacag atttcaatga aaataaatta gagagcataa
     6721 atattttgaa aggtttcaag gcgttgagac tgacagaagg attttcggag ttcaagcatt
     6781 tgaaaaggag tggttatagt tttcatcaga atatgattta ttttttaaag gtctgatgga
     6841 acccacatgt ctgaatatta tatttattcg
//
LOCUS       DAOSIB010000094         6779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_10194_length_6779_cov_11.276472, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000094 DAOSIB010000000
VERSION     DAOSIB010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6779
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10194_length_6779_cov_11.276472"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..1370
                     /gene="thrC"
                     /locus_tag="PLD44_07735"
     CDS             36..1370
                     /gene="thrC"
                     /locus_tag="PLD44_07735"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOF99257.1"
                     /translation="MKYYSTNKGISGVSLKDAVIKGLADDKGLFMPEQIKQLPPSFFD
                     TIINMSFQEIALAVAEAFFGEDVDNEALKNIVYETLSFDVPLVHVKDNIFSLELFHGP
                     TLAFKDVGARFMSRLLRYFIQKEGEKNVNVLVATSGDTGSAVANGFLGVEGIHVFVLY
                     PKGLVSPIQECQFTTLGKNITALEIDGTFDDCQRLVKNAFADKELNSSLKLTSANSIN
                     VARFLPQAFYYFYAYAQLKAIHPKFTQEELEMVICVPSGNFGNITAGLFAKEMGLPVK
                     RFIAANNRNDIFLQYLKTGKYQPRPSVQTIANAMDVGDPSNFARILDLYHGSHDKISS
                     LISGVSYTDEEISATIKECYRETGYLLDPHGACGYRALRDNLTNNENGIFLETAHPAK
                     FLDTVEKIIGEKVEIPAKLQEFMKGEKQSIPLKNDFESFKNFLLSAALGNKD"
     gene            complement(1380..1826)
                     /locus_tag="PLD44_07740"
     CDS             complement(1380..1826)
                     /locus_tag="PLD44_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HOF99258.1"
                     /translation="MLEFTELGYLGLFIGSFLAATVVPFSSEVIFSTMVFGGLNAWTC
                     VFVATAGNWLGGMTSFYLGKLGKIEWIEKYFHVKKEKIDQFLEKFHKYGEWFVFFSFL
                     PFIGDAIAIAAGFLRARWWATAAFMLAGKFCRYVVWMYVNGLILMI"
     gene            complement(1833..2828)
                     /locus_tag="PLD44_07745"
     CDS             complement(1833..2828)
                     /locus_tag="PLD44_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99259.1"
                     /translation="MGRRQISIKSLITPTIPGTVLLVLFCVGWWYFPYLIGKQPFMLH
                     DDPPFVLSVFSFNPAINNFLSLLLTVLNAILIQNLNLRFNILRKRSLLPFLIFLFLMA
                     GWYPLHFIWELHLALSIFILAFYYFFDMYRQPAAVEQAFMGSFLIAVASLFLPPLLWM
                     VPVVWINFLQMRAFTLRTFLASILGILSPWILYAGGYFVFQDNFDFNKLIFSRFFDGG
                     KYDFSSFQSIDLIYFVSLAAVLLISIVYIYMNFQQDSISTRSKLNMIIIQMLFLLGIS
                     IFHASVFYCFLPFAVFFYVLLLSHPFSTRENNFFSILFLVFCLLNVAFVLFKIIA"
     gene            complement(2862..4958)
                     /locus_tag="PLD44_07750"
     CDS             complement(2862..4958)
                     /locus_tag="PLD44_07750"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOF99260.1"
                     /translation="MKKLIVAVMIGTLLTGCSTNKDNPFLQVYDTPFSVPPFDKIKTE
                     HYLPAFREGIKTHQKEIDAIAENSEAPTFANTVEALDFSGELLKKVSSVFFNLYSTDT
                     NDKMEAIANEVTPLLTEHSDNLYLNKALFQRIKTLYDKRSTLGLNAEQIRLLEKYYKN
                     FVRNGAALNDEQKERLREINKQLSLAELQFGQNVLAETNAYQKWITDEKELVGLPENV
                     KQSAAEAAKEAGREGQWLFTTKKASFIPVLQYSENRELRRELLTAYNKLADNGNKNDN
                     KPVINKIVKLRVQKAQLLGYPTWADYELDDTMAKTPQKVYEFLASVWEPSVRKAREEA
                     AELQKMMDAEGKGEKLEPWDWWFYAEKLRKAKYDLEEEALKPYFKLENVRKGVFDLAS
                     KLYGLKFKKLENMPVYHPDVEVFEVTDANDSLIGILYTDYYPRAGKKAGAWMNNISEQ
                     YIKDNINHRPVICNVGNLTKPTSDKPALLSMDDVETLFHEFGHALHGLLSQCTYPSLS
                     GTNVARDFVELPSQIMENWCWEPEVIKTYAFHYKTGEVMPNELIEKIQQASTFNQGFV
                     MTELLSAALLDMDYHTIKDTADIDVTAFENATMKRIGMIPQIVVRYRSTYFNHVFSGG
                     YSAGYYSYTWAEVLDADAFQAFKETGDIYNSEIAASFRKNILERGDSDDPMKLYVAFR
                     GKEPQPDALLIKRGLK"
     assembly_gap    5186..5285
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5286..5899)
                     /locus_tag="PLD44_07755"
     CDS             complement(<5286..5899)
                     /locus_tag="PLD44_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HOF99261.1"
                     /translation="MQVIEANSGIKIEPCVATVGFFDGVHIGHRFLMEELKKIASTQH
                     LKSVVVTFDQHPRKVLNADFQPKLLTTLDERLFQIGSTGVDACVVLNFTREMSLLSAR
                     DFLKEVLHDQLNVQTLLVGHDHRFGHNREDGLSEYIRYGEEFGIKVIEAQRFATEDMP
                     HISSSVIRRALLDGDVETANRLLMYEYSFEGKVVNGFKVGHKIG"
     gene            complement(5968..6609)
                     /locus_tag="PLD44_07760"
     CDS             complement(5968..6609)
                     /locus_tag="PLD44_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOF99262.1"
                     /translation="MIHFKNISALIFDFGGVLINLSRQQCIDSFRSLGVKEVEKYLDD
                     FNQSGFFLQLEQGKISPEQFRNEVRKLSDHTLTDEQIDRAFCSFLLDIPEEKLKLLLE
                     LRKRFKIYLLSNTNLIHMTFCRAQHFHYNGYSMDDFFDKCYFSYELGITKPDIRVFNS
                     VIEDIGLPPQQCLFLDDGLQNIEQAQKTGLQTYWVKQQEDLSFLLQPDVFVFD"
     gene            6704..6778
                     /locus_tag="PLD44_07765"
     tRNA            6704..6778
                     /locus_tag="PLD44_07765"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6739..6741,aa:Ile,seq:cat)
BASE COUNT         2162 a         1414 c         1189 g         1914 t
ORIGIN      
        1 ttccatcaga ccattaaaaa ataaattata ttctgatgaa atactacagc accaacaaag
       61 gaatttcggg tgttagtctg aaagatgccg ttatcaaagg cttggccgac gacaaaggac
      121 ttttcatgcc ggagcaaatc aagcagttgc ctccttcttt tttcgatacc atcatcaata
      181 tgtccttcca agaaatagca ttggcagtgg cagaagcatt ttttggagaa gatgtagata
      241 acgaagcact gaaaaatatt gtgtacgaaa ccttgtcgtt tgatgttccg ttggtgcatg
      301 ttaaagacaa tatcttcagt ctggaacttt ttcatgggcc aacgctggcg tttaaagacg
      361 ttggtgccag attcatgtcc cgtttgctcc gctattttat ccagaaagaa ggcgaaaaga
      421 acgtaaatgt attggttgcc acttccggcg acactggaag cgctgttgcc aacggttttt
      481 taggcgtaga aggcatacac gtttttgttc tttacccgaa aggattggtc agtcccattc
      541 aggaatgtca gtttaccact ttaggaaaaa atattaccgc gctggaaata gatggaacat
      601 tcgacgactg tcaacgtctg gttaaaaatg cttttgcaga taaggaattg aattcttccc
      661 taaaactaac ttcggcaaac tctattaatg ttgcccgatt ccttccacag gctttttatt
      721 atttttatgc ttatgcacaa ttaaaagcca ttcatccaaa gttcacacag gaagaattgg
      781 aaatggttat ttgcgtacca agtggaaatt ttgggaatat tactgccggc ctttttgcca
      841 aagagatggg attgcctgtg aaacgcttta ttgctgccaa caaccgcaac gatatttttc
      901 ttcaatatct gaaaaccggt aaataccaac cacgtccttc cgtacaaacc attgcaaacg
      961 ctatggatgt aggcgaccca agcaattttg cccgtattct cgatttatat catggttcgc
     1021 atgataaaat cagttctttg atttcgggtg tcagctatac cgacgaagaa atttctgcca
     1081 ccattaagga atgttatcgt gaaaccggtt atttgcttga tcctcatgga gcttgtggct
     1141 atagagcttt gcgcgataac ttgacaaata acgaaaacgg aatttttctg gaaacagctc
     1201 atccagctaa atttttggat acggtagaaa aaataattgg agaaaaagtt gaaattcctg
     1261 ccaaacttca agaatttatg aaaggcgaaa agcaaagcat tcctttaaaa aacgatttcg
     1321 aatcgttcaa aaactttctt ttatcggctg ctttggggaa taaggattga caacatttgc
     1381 taaatcatta atatcaaacc gttcacatac atccaaacga cgtaacggca aaatttcccg
     1441 gcaagcataa aagctgccgt tgcccaccag cgggcacgta aaaaaccggc tgcaatagct
     1501 atggcatcgc caatgaaagg caggaaagag aaaaacacaa accattctcc atatttgtga
     1561 aatttttcca gaaattggtc aattttttct tttttgacat ggaaatattt ttctatccat
     1621 tctattttcc ccaatttccc caaataaaaa gatgtcattc ctccaagcca gttgccagct
     1681 gtagccacaa aaacacaagt ccaagcattc aaaccaccaa aaaccattgt ggaaaatata
     1741 acttccgaac tgaaaggaac caccgttgct gcaagaaatg accctataaa aagtcccaaa
     1801 taacccaatt ccgtaaattc caacatgagt ggttaggcaa ttattttaaa caacacaaag
     1861 gccacattta gcaagcaaaa aacaaggaac aaaatgctga aaaaattatt ttctctcgta
     1921 gaaaaaggat gagacaaaag caatacatag aaaaaaaccg cgaagggaag gaaacagtaa
     1981 aagaccgaag catgaaaaat ggaaattccc aacaaaaaca acatttgaat aatgatcata
     2041 ttcaacttcg accgcgtgct aatgctatcc tgctgaaaat tcatgtagat ataaactatc
     2101 gaaatcaata aaactgcggc cagtgaaaca aaataaatca aatcaatgga ttgaaatgaa
     2161 gaaaaatcat attttcctcc atcaaaaaaa cgactgaaaa tcaatttgtt gaagtcaaaa
     2221 ttatcctgaa aaacaaaata cccacctgca taaagtatcc acggactcaa tatccccaaa
     2281 atggaagcaa gaaaagttct cagcgtgaaa gctcgcattt gtaaaaaatt aatccaaacc
     2341 accggcacca tccagagcaa aggtggcaaa aacagacttg caacagctat cagaaagcta
     2401 cccatgaaag cctgctcaac agcagccggc tgacgataca tgtcgaagaa ataataaaaa
     2461 gccagaataa aaatgctcaa tgccaaatga agctcccaaa taaaatgcaa cggataccat
     2521 ccggccatta aaaacaaaaa aatcaaaaaa ggcaataaag acctttttct gagaatattg
     2581 aagcgtagat tcaaattctg aatcaatatc gcattcaata cagttaaaag caaacttaaa
     2641 aaattgttaa tagccggatt gaatgaaaaa acagaaagaa caaacggagg gtcatcgtga
     2701 agcataaatg gctgcttccc aatcaaatag gggaaatacc accagcctac acagaacagt
     2761 acaagcagca ctgttccggg tatagtagga gttattaaag atttaatgga tatttgacgc
     2821 ctgcccactg aaagtttttt taaaaatttt cctcttcaac attattttaa tcctcttttt
     2881 atcaaaagag catcgggttg aggttcctta ccgcgaaaag ccacatacaa cttcatggga
     2941 tcatccgaat cgccccgctc aagaatattc ttacgaaaag aagcagctat ttcggaatta
     3001 tatatatcgc cagtttcctt aaaagcctga aaagcgtcgg catcgagtac ttcagcccat
     3061 gtatagctat agtatccggc agaatatcct cccgaaaaaa catggttgaa ataggtactc
     3121 cgataacgca ccactatttg aggaatcatg ccgatgcgct tcatggtggc attttcaaaa
     3181 gcggtaacat ctatatcggc ggtatcttta atggtatgat aatccatatc gagcaatgct
     3241 gccgaaagca gttccgtcat gacaaacccc tgattgaaag tacttgcctg ctgaattttc
     3301 tctatcagtt cattgggcat cacttctccg gttttataat gaaaagcata agtttttatt
     3361 acttccggtt cccaacacca gttttccata atttgtgaag gaagttctac aaaatcacga
     3421 gctacgtttg tacccgaaag agaaggataa gtgcactgag aaagtaaacc gtgcaaagca
     3481 tgtccaaact catgaaataa agtctccacg tcgtccatgc tcaacaaagc aggtttgtcg
     3541 gaagtgggct tggtcagatt gccaacatta caaataacgg gtcgatgatt gatattatcc
     3601 ttgatgtatt gttccgaaat gttgttcatc caagcgcctg cctttttgcc ggcacgagga
     3661 tagtaatcgg tatagagaat accgatcagc gaatcgttgg catcggttac ttcaaaaact
     3721 tcgacatcgg gatggtaaac cggcatattt tcgagttttt taaacttcaa tccgtaaagt
     3781 ttggaagcta aatcaaaaac cccttttctg acattctcga gtttaaaata tggttttaat
     3841 gcttcttctt ccaaatcgta tttggctttt cgaagttttt cggcataaaa ccaccaatcc
     3901 cacggttcca atttttctcc ttttccttcg gcatccatca ttttttgcaa ttctgccgct
     3961 tcttctcgtg ctttacgtac ggaaggttcc cacacggaag ccaaaaattc ataaactttt
     4021 tgaggtgttt tggccattgt atcgtccaat tcataatcgg cccaagtggg ataacccaac
     4081 aattgggctt tttgcacgcg gagtttcaca attttattga tcaccggttt gttgtcgttt
     4141 ttgttgccat tatcggcgag cttgttataa gcggtcaata attctcgacg caattcacga
     4201 ttttcgctat attgaagaac gggaatgaaa ctcgcctttt ttgtggtgaa aagccattgt
     4261 ccttctctcc cagcctcttt ggcagcctca gctgccgact gtttgacatt ttccggcaat
     4321 ccgaccaatt ctttttcgtc agtaatccat ttttgataag catttgtctc tgccaataca
     4381 ttttggccaa actgcaactc tgccaaacta agctgtttgt tgatttctct caatctttcc
     4441 ttttgctcat cgtttaatgc agcaccattg cgaacaaaat ttttgtaata cttctccagc
     4501 aacctgattt gttcggcatt gagcccaagt gtggaacgtt tatcgtaaag ggttttaatt
     4561 cgttgaaaca gcgccttatt gagataaaga ttatcactgt gttccgtcag aagcggcgtt
     4621 acctcgttgg caatggcttc cattttatcg ttggtatcgg tagaatagag attgaaaaat
     4681 acggaagaaa cttttttcag caattctcca ctgaaatcca acgcttcaac cgtattggca
     4741 aaagttggcg cttcgctatt ctcggcaatg gcgtcaattt ccttctgatg agtttttatt
     4801 ccttcacgaa aggcaggaag ataatgttca gtcttgattt tatcaaatgg aggaacgcta
     4861 aaaggagtat cataaacctg caagaaagga ttgtccttgt tggtagaaca accggttaat
     4921 aatgtaccaa tcataacggc aacaattaat tttttcataa ataaatgatt ttaacaaatt
     4981 tgtcgataaa tattttcggt taaataagat ttttgcttct caattgctat gttttataca
     5041 gcaaaacgaa aaattgtttg ctttcctttc aaaaatacaa cgaaagcatg aaaaatgaaa
     5101 tttcagaaat tcattgccaa aacttcctcc ctgtcccgat acaactgctc aatcagttct
     5161 tctaccgtat cgaatttaat ttcatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnaatcc aattttatga cctaccttaa agccatttac aacctttcct tcaaaagaat
     5341 attcatacat caacaatcga tttgccgttt ccacatctcc atcgagtagt gctcgacgaa
     5401 taacggaaga actgatatga ggcatatctt ctgtagcaaa ccgctgagct tctataactt
     5461 ttattccaaa ttcttctccg tagcggatat actccgacaa tccatcttcc cgattatgac
     5521 caaaacgatg gtcgtgcccc accagcagag tctgtacatt gagttgatcg tgaagaacct
     5581 cttttaaaaa atcccgcgcg gacaacaagg acatttcacg cgtaaaattc agcaccacac
     5641 aagcatctac acctgtcgaa ccgatttgga aaagtctttc gtccaacgtg gtgagcaatt
     5701 ttggctgaaa atcggcattt aaaactttac gcggatgctg gtcgaaagtt acaaccacgc
     5761 ttttcaaatg ttgcgtcgaa gctatctttt tcaattcttc cattaaaaat cgatgaccaa
     5821 tatgcacgcc atcaaaaaaa cctactgtgg caacacaagg ttctattttt attccgctgt
     5881 tcgcttctat cacttgcatg aaattcccac taaaatgaaa tacattgaaa atttatttgg
     5941 aaaaacctat tgatttttct acttgtttta atcaaaaaca aaaacatcgg gttgaagcag
     6001 gaaactcaaa tcttcctgtt gtttcaccca ataagtttgc aagccggttt tttgtgcctg
     6061 ttcaatattt tgcaacccat catccaggaa aaggcattgc tgcggtggta gcccaatatc
     6121 ctctatcacg gaattaaaca cacggatgtc gggcttggtt attcccaact cataagaaaa
     6181 ataacatttg tcgaaaaaat catccatcga ataaccgtta taatgaaaat gctgtgctct
     6241 gcaaaaagtc atatgaataa gattggtgtt gctcaacaaa taaattttaa accttttccg
     6301 aagttccaac aacaacttta atttttcttc cggtatatcc agcaaaaatg aacaaaaagc
     6361 tcggtctatc tgctcatcgg tcaaagtgtg gtcagataat tttctgactt cgttgcggaa
     6421 ttgctccggt gaaatttttc cctgttccaa ttgaagaaaa aaaccggact gattgaaatc
     6481 gtccagatat ttttccactt cttttactcc cagagaccga aaactatcaa tacactgttg
     6541 acggctcagg ttgatcaaca ccccgccaaa atcgaatatc agagccgata tatttttaaa
     6601 atgtatcatt taaaagaaaa ataacaaaga attttttcaa acacacaaaa aattattatt
     6661 tttgcattcg ctttgcaaag ataagcaatt attcgcaaat gcagggccca tagctcagtt
     6721 ggttagtagc acctgactca taatcagggg gtcgctggtt caagcccagc tgggcccac
//
LOCUS       DAOSIB010000095         6723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_10309_length_6723_cov_7.287642, whole genome shotgun sequence.
ACCESSION   DAOSIB010000095 DAOSIB010000000
VERSION     DAOSIB010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10309_length_6723_cov_7.287642"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..6401)
                     /locus_tag="PLD44_07770"
     CDS             complement(210..6401)
                     /locus_tag="PLD44_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOF99263.1"
                     /translation="MKMHCYKRESLSFPRISMLFLLISLSSFVFSLTYNVTVPAGTKF
                     CYIAGEMNGWSQQVMNKVDETHYTLNMPSATSNQLYKYCSGPSWAYVENIANNRSYTA
                     NDVVTSWAAVYDPSAIPVDVVYTVTVPAGTYTCYFAGGATNWVHKQMAKIDDTHFTTT
                     INTTLPNGYKYCSGPEWKYEELDANNQSISDRNYNKSDVVINWKEVYNPNPNGLIYNV
                     TVPAGTKACYIAGEMNNWTQQAMNKVDEAHYSISIDNATAAMKYKYYSGPDWNFGELN
                     ADNSAVANRNYSSSDVVAKWAVVWDPNVSILSISNSLILSSGKVDRYSFNNDLIGVRT
                     VDVWLPDGYAPSKKYAVVYMHDGQMLFDATSTWNNQEWKVDETLSQLMQNGTIKDVIV
                     VGIWNSSNRYAEYYPEKSMDYLTSSVKTAKLNEMNNDPKGDEYLSFIVNQVKPFIDLT
                     YSVYTNAANTFVAGSSMGGLISWYALCEYPNIFGGAVCMSTHWGVIGTDDPSIPDSFR
                     KYLLAKLPSASNHKIYFDHGTVGLDANYDANQQLVDTIMKYKGYSSSNWETLVFQGDD
                     HNEVSWAYRFNVPMKFMLAKNSEISGLNLNTENLNGKGFLCGSTVVLNWTSNGIQNIK
                     IEYSPDNGSNWTVIASSVDASLKTFSWTVPTFTSTTCKIRISDVTNAGTKSESDLFSI
                     APAITYVTFSVDASILINDGKFNATTDKMYIRGSFNGFDLTHELNLGNNNIYSLTIPL
                     PSNTYVGYKYFSTTVNAENGGWENNFPVTSSGNRSIYVGVKNLTLTTVFYNDGDMNLS
                     KSSDHFNVIYSAQDNNLITQFIERIELFHSIVSSSLGVNPTEKTNIFLYKDLDHLHLA
                     QGYPENDENSTGSAHGKILFIMLSPAKMGLNEGLGLFAHEYTHCMNAWKTKVTLPAWL
                     NEGVACYFGRQISTKDWIKSITDQRGKPVIQDIFNTDMGYAYSFIVAYYIIKTKEMPA
                     MAKFIENMNYSDIGYSGLPALQNDWNNFLDVYLNTQTNVKVKLSVNMSDMIAAGYFNP
                     ATDKVYVKGDWNWWNGQQMTLETGNIYSVSVPINTYSLNEYKFYTNSATAPNNGLELN
                     TDETISGNRLLDIFNEPKTLNIATFKSNAIPSIANVDMKKISDKVQVLIFHGRIWNAP
                     SFNTFKYPFKLITSTDYQSLKPSDAFAFDAGFIGTNDTIYISEPVTTEQIATFNNVTN
                     VALYYLCQAYLYHYYQTRNMPLIFKIGFPLFEAGLMPSDETVKTAVNAYGGSFASFDA
                     LNNRSTFVSNNGKAVAAAFGEFMNIFKNWGYGMISSMNANSFDVLSYWWNVNDLPGLL
                     GDFNRYVNYRFLQTDNNFRVQMVKETEHFKFYTRPVDYALNFPYFSDVLEPAFVEYTT
                     NFNLNITEKFSYFTLPTCIDGEIEGVPCNGLPPSRITGGTAWSSGMHSTCASTQDELP
                     LFYHMNRHELAHGMQGNMPQGIVTAWLNEGFPEFCARGPITDTKLIEMAQSGIDCMNA
                     AVNYFGHLPTYEETRIYPSPDYGYYTLGYFLIDYQYRRGGYPLVKAIQMNDLAAYQSL
                     GYSTSQAFLEDFYFDFDVRVKKLPVATLIKPVTNMDENNPTVQIAWTPLKAGVKLNVY
                     VSVDDAKTWTKIADHITENSCTWNAGNIISRFYLKIEAPDNMNLSTTYGPFVKGDLTK
                     LNIISPMIDNYAIAGDTMNIKWGRTNIQRIKLEYATNVVPASQVNASDWKTITGDIST
                     TASNYQWAIPATLEGTCKLKITDTSNANNYSESEIFNLVKPNETGGPYLYDKNTLLLL
                     HFDNDLKNRSLATGNAVGDVQNLSATDNVSPQFGKSYRTVSNLTVPHHVNLNLTGDWT
                     LEAWVKLNSFTGNSMYLFWKPGDTDSYFSNYALEISPWWGNIFFGYYFSQAKDRIGLT
                     TTFSPSLNEWYHVAYIRNTAKNQLQLIVHDKNRNLISSVEMNYSANETLLNSKDLIIA
                     ANLDGYMDEVRISNVVRNFINTGVNKTFENQVTNFYPNPAKEWITIKNTSGAYKIEIT
                     DLQGKILISRKLENLDERINISSLLDGIYLLKLVGDKGISISKLIVE"
BASE COUNT         1992 a         1284 c         1291 g         2156 t
ORIGIN      
        1 ttataacgta aatataatga atatcagcga attatacaaa tttttataac tattctctga
       61 aaaatatacc ataatttatc ccttttaatt taggaaagtt tgcggataat cataaaattt
      121 tttttgaact cgttactaac gataaagcgt ctgaaaaatt attgattttt tcaggcgctt
      181 tatcgtttat gcaattcatc ggttattttc tattccacaa tcaatttgct gatggagatt
      241 cctttatcgc caacgagttt taacagataa attccatcta gcaaagaaga gatattgatg
      301 cgttcatcca aattttctaa ttttctactt atcagaattt ttccttgaag gtcagtgatt
      361 tcaattttgt aagcgcctga ggtgttttta attgtgatcc attcttttgc agggtttgga
      421 tagaagttcg tgacttggtt ctcaaaagtt ttgtttacgc ccgtgttgat gaaattcctc
      481 accacattac tgatgcgtac ttcgtccatg tatccatcca aatttgctgc gataatcaag
      541 tctttggaat tcagcaacgt ctcattcgca gaataattca tttcgacgga agaaatcaag
      601 tttcgatttt tatcgtggac aatcaattgc aactggtttt ttgccgtatt tcggatataa
      661 gctacatgat accattcgtt caatgacgga ctgaaagtcg tcgtcaaacc gattcggtcc
      721 tttgcttgcg agaaatagta gccaaaaaag atatttcccc accacggact gatttctaat
      781 gcgtagttgg aaaagtagga atcagtatcg ccaggtttcc agaaaagata catgctgttg
      841 ccggtaaaag agttcagttt gacccatgct tcgagtgtcc aatcccccgt aagattcaaa
      901 ttgacatgat gcggaacggt caagttcgaa accgttcgat agcttttgcc aaattggggt
      961 gaaacattgt ctgtggcaga aagattttga acatcgccaa ccgcatttcc ggtagcaagg
     1021 cttctgttct tcaagtcgtt gtcgaaatga agcaacaaca atgtgttttt atcgtaaaga
     1081 taaggaccgc ctgtttcatt cggttttacc aaattgaaaa tttcgctttc ggaataattg
     1141 tttgcatttg aagtgtcggt aatcttcagt ttacaagttc cttcaagcgt cgcaggaata
     1201 gcccattgat aattggaagc cgtggtcgaa atatcgccgg ttatcgtttt ccaatcagaa
     1261 gcattgacct gtgaagcagg gacaacattc gttgcatatt ccagtttaat ccgttgaatg
     1321 ttagttcttc cccatttaat gttcattgta tctccagcaa tggcataatt atcaatcatt
     1381 ggagaaataa tattcaattt cgttaaatct ccctttacaa aaggcccgta tgtggtggag
     1441 agattcatgt tatcgggtgc ttcgattttc aaataaaatc ggctgataat atttccggca
     1501 ttccaagtgc aagaattttc ggtaatatga tcggcaatct ttgtccatgt ttttgcgtca
     1561 tccactgaaa cgtacacgtt cagtttaacg cctgctttga gaggagtcca tgctatttgt
     1621 acagtcggat tgttttcatc catattcgtt accggtttta tcaaggtagc aaccggaagt
     1681 ttttttactc tgacgtcaaa atcgaagtag aaatcttcca gaaatgcttg cgaggttgaa
     1741 tatccgagcg attgataggc tgctaaatca ttcatttgta ttgcttttac aagcggatat
     1801 ccacctcttc gatattgata atcaatcaga aaatatccca gcgtgtagta tccgtaatcc
     1861 ggcgaaggat agattcgtgt ctcttcgtac gtcgggaggt gtccgaaata gtttacggct
     1921 gcattcatgc aatcaattcc gctttgagcc atttctatga gtttcgtatc cgtaatcggt
     1981 cccctggcgc aaaactcggg aaatccttcg ttgagccatg cggttacaat tccttgcggc
     2041 atatttccct gcattccatg agccagttcg tgccgattca tatgatagaa taagggtagt
     2101 tcatcttgcg ttgaggcaca agttgaatgc attcccgatg accatgctgt accgccggta
     2161 attcttgagg gaggcaatcc attgcaggga acgccttcta tttctccatc aatgcatgtt
     2221 ggcaaagtaa agtaggaaaa tttttcagta atattcaaat tgaaatttgt cgtatattca
     2281 acgaaagcgg gttccaaaac atcgctgaaa tatggaaagt ttaaggcata atcgaccgga
     2341 cgcgtataaa atttaaaatg ttctgtttct ttcaccattt gaacccggaa attattatcg
     2401 gtctgtaaaa accgataatt tacgtaccga ttaaaatcgc ctaacaaacc cggtaaatca
     2461 tttacattcc accaatagga gagaacatca aaactgtttg cattcattga acttatcatc
     2521 ccatatcccc aatttttaaa tatgttcata aattccccaa atgcggcggc aactgccttt
     2581 ccgttgtttg aaacgaaagt agaacgatta ttcaatgcat caaaagaagc aaaactgcct
     2641 ccataagcat taacggctgt ttttaccgtt tcatcggaag gcattagtcc tgcctcaaaa
     2701 agaggaaatc cgattttgaa tatcaacggc atatttctcg tttggtaata atggtaaaga
     2761 tatgcttgac acaaataata taaagccaca tttgttacat tgttgaaagt tgcaatttgt
     2821 tctgttgtta ccggttcaga gatgtaaatc gtgtcgtttg tacctataaa tccggcatca
     2881 aaagcaaatg catccgaagg ttttagagat tggtagtcag tagaagtgat tagtttaaat
     2941 ggatatttga aagtattaaa tgaaggagca ttccaaatgc gaccgtgaaa tattaggact
     3001 tgaactttat ccgatatttt tttcatatct acatttgcta ttgacggaat agcattggat
     3061 ttgaaagtag caatattgag cgttttcggt tcgttgaaaa tatcgagcaa ccggttacca
     3121 ctgatagttt catccgtatt cagttccaat ccgttatttg gagctgtagc cgaattggtg
     3181 taaaacttgt attcattcaa actgtacgta tttataggaa cagaaacgga ataaatattt
     3241 ccggtttcga gtgtcatttg ttgtccgttc caccaattcc aatcaccttt gacgtaaact
     3301 ttgtcggtag cagggttaaa atatcctgca gcaatcatat cggacatgtt gacgcttaac
     3361 ttaaccttca cattcgtttg cgtatttaaa taaacatcca aaaaattgtt ccaatcgttt
     3421 tgcaatgcag gcaggccgct atatccaata tcggaataat tcatgttttc aatgaatttt
     3481 gccattgcag gcatttcttt cgtcttaata atatagtaag caacgataaa cgaataagca
     3541 taacccatat ctgtgttgaa aatatcctga ataaccggtt tacctctttg atcggttatt
     3601 gattttatcc agtcttttgt tgaaatttga cggccaaagt agcaggcaac tccttcgttc
     3661 agccatgcag gtaaagttac ttttgttttc cacgcattca tgcagtgagt atattcgtga
     3721 gcaaataatc ccagaccttc atttaaaccc atttttgcag gcgaaagcat gataaacaga
     3781 atttttccgt gagccgaacc cgttgagttt tcgtcatttt caggataacc ttgagctaaa
     3841 tgtaaatgat ccaaatcctt ataaagaaaa atattggttt tttcagtagg attcactccc
     3901 agagaagaac taacaatcga atgaaacagt tcgattcttt ctataaattg ggtgatcaaa
     3961 ttattgtcct gagccgaata aatgacgttg aaatgatcac tcgatttgga tagattcata
     4021 tcgccgtcgt tgtagaaaac ggtcgtcaat gtaagattct tcacgccgac ataaatgctt
     4081 ctgtttcccg aagaggtaac tgggaaattg ttctcccagc cgccattttc tgcattcacc
     4141 gttgtagaaa aatacttata tccaacgtaa gtattcgatg gaagcggaat agtcaacgaa
     4201 tagatattgt tgtttcctaa atttaattca tgcgtcaagt caaatccatt gaatgagcct
     4261 ctgatataca ttttatcagt tgttgcattg aatttaccgt cattgatcaa aatgcttgca
     4321 tcaactgaaa acgtaacata tgtaattgcc ggagctattg agaataaatc gctttccgat
     4381 tttgtgcctg cattggttac atccgaaatc ctgattttgc aagtggtgga agtgaatgtt
     4441 ggaactgtcc aactaaaggt tttcagcgaa gcgtctacgg atgaagcaat aactgtccag
     4501 ttcgaaccat tgtcgggaga atattcgatt ttaatgtttt gaattccgtt tgaagtccaa
     4561 ttcaaaacaa cagttgaacc acataaaaac cctttaccat tcaggttttc tgtattcaaa
     4621 ttaagcccag agatttcact gttttttgca agcatgaatt tcataggaac attgaaacgg
     4681 tatgcccaac tcacttcgtt gtgatcatct ccttggaaca ccagtgtttc ccaattcgac
     4741 gacgaatatc ctttgtattt cataattgta tcaaccaatt gctgatttgc atcgtagttg
     4801 gcatccaaac caaccgttcc gtggtcgaaa tagatcttgt gattggaagc ggagggaagt
     4861 tttgccaaca gatattttcg gaatgaatcg ggaatcgaag ggtcatccgt accaataact
     4921 ccccaatgcg tggacataca gacagcacca ccaaaaatgt ttggatattc gcaaagtgca
     4981 taccatgaaa tcaatccgcc catactcgat ccagcaacaa acgtattggc tgcattcgta
     5041 tacacggaat aggtgaggtc gataaacggc ttcacctgat tgacaatgaa cgaaaggtat
     5101 tcatcgcctt tcgggtcgtt gttcatttcg ttgagtttgg cagttttgac acttgaagtc
     5161 agataatcca tcgatttttc gggatagtat tctgcatatc ggttcgaact gttccaaatc
     5221 ccaacgacaa tcacatcttt aatcgttcca ttttgcatca attgagagag tgtttcgtct
     5281 actttccatt cctggttgtt ccaagtggac gttgcgtcaa atagcatttg tccgtcgtgc
     5341 atgtatacaa ctgcatattt tttcgaagga gcatatccgt caggaagcca aacatcaact
     5401 gttctcactc cgattaaatc gttgttgaac gaataccggt ctactttacc agatgaaagt
     5461 atcagtgagt ttgaaattga taatatgcta acatttgggt cccaaacgac agcccatttg
     5521 gcaactacgt ctgaagaaga ataatttcga ttagcaacag cggaattatc ggcattcaat
     5581 tctccaaaat tccaatccgg tcctgagtag tatttgtatt tcatagccgc agttgcattg
     5641 tcgatcgaaa tggaataatg cgcttcgtcc actttattca tcgcttgttg tgtccaatta
     5701 ttcatctcgc cggctatgta acaagctttt gtcccagcag gaacggttac attatagatc
     5761 agtccgtttg gatttggatt gtaaacttct ttccagttaa ttaccacatc cgatttgttg
     5821 taattcctgt cggatatcga ttggttgtta gcatccaatt cttcatattt ccattccggc
     5881 cccgaacaat atttatatcc gttcggtaag gttgtattta ttgtagttgt aaaatgagta
     5941 tcatctattt ttgccatttg cttgtgtacc caattggtgg ctcctccggc aaaataacac
     6001 gtgtaggtcc cggcaggaac cgttactgta tagacaacat cgaccgggat ggctgaagga
     6061 tcgtaaacag ccgcccatga agttacaaca tcattggctg tatagcttct gttattagct
     6121 atgttttcga cataagccca cgaaggtccc gaacagtatt tgtacaattg attagaagtg
     6181 gctgaaggca tattcaacgt gtaatgcgtt tcgtcaactt tattcatgac ttgctgcgac
     6241 cagccgttca tctcgccggc aatgtagcag aattttgtac cggctggaac ggtaacatta
     6301 tatgtaagac tgaaaacaaa acttgagagg gaaatcagta aaaacagcat ggaaattctt
     6361 gggaaagaga gagattctct tttgtagcaa tgcattttca tagttttaaa attaagggaa
     6421 ttgattaaaa aaattaaaag aagagaaaag agatataaaa tcggccaaag ataatcagat
     6481 ttatagaaaa aattatatgt tttgcataaa aattttaatt ttttacaaaa tattttttca
     6541 tcaaaatatt ttcatattaa aattcgtctc atattattga ttatcaagta aatttaaaac
     6601 tgaaaatcaa ggaattctga ttggctgcaa ttatttattt agataaaaaa atgcgtggaa
     6661 atttttggag attttgctgg aaaatattgc agaagtattg ttcagaagag aaagcagccg
     6721 gcc
//
LOCUS       DAOSIB010000096         6433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_10905_length_6433_cov_12.929288, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000096 DAOSIB010000000
VERSION     DAOSIB010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10905_length_6433_cov_12.929288"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PLD44_07775"
     CDS             complement(<1..305)
                     /locus_tag="PLD44_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007748570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOF99264.1"
                     /translation="MKKFLFVLMTVAFLGCRSEKPATPAMETFPYTVDRFADIEILRY
                     RVPDFETLTLQQKELIYYLSEAALSGRDILYDQNNKFNLCVRRTLEAVYENYRGDRQS
                     "
     gene            complement(395..1147)
                     /locus_tag="PLD44_07780"
     CDS             complement(395..1147)
                     /locus_tag="PLD44_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOF99265.1"
                     /translation="MRIGLIGFGKTGKAVASVILQSESFELKWILRRKKLLDHRSAAE
                     VLGIETSSTEGLIYSTSNTNLENLMDTLPVDVIIDFSSGQGIYTYGELAAKRKIKIIS
                     AISHYKPKEVAFLHKLAQQTTVFWSPNITLGVNYLMISAKFLKKIAPWVDIEIVEEHF
                     KGKDGISGTALKIAEVLDMDKAKINSIRAGGIVGRHEIIFGFPYQTVRLIHESISREA
                     FGNGVLFVAENLKDKEDGFYNFQDLLLPYFYD"
     gene            complement(1258..2805)
                     /locus_tag="PLD44_07785"
     CDS             complement(1258..2805)
                     /locus_tag="PLD44_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HOF99266.1"
                     /translation="MRKDRILWIDDEIEMLRPYLLFLDEKGYEVVTATNGNDAVEICQ
                     EENFDIIFLDENMPGLSGLETLALIKEIDPDVPMVMITKNEEENIMNMAIGNKIADYL
                     TKPVNPSQILMTLKKNIHRKEIVNEHATAAYRSEFGQIGMKINDSFTYDDWVEVYKKL
                     VFWELELSETESGMDELLRMQKMDANTTFSKFIKNNYERWFERPEERPLMSFEIFKNK
                     VFPLLDNGEKVFFVVVDNFRLDQWKMIRELLSDYYQFDEEDLYCSILPTATQYARNAI
                     FSGLMPLQIQQMFPQFWVEEEEEEGKNLMESELLQTQIQRYRKKYTFSYHKINDSVSC
                     QHLIDQLPHLENNNLNVCVLNFIDMLSHARTESKMVRELANDEKAYRSLTLSWFRHSA
                     TFDLFRAIAERGYKVVFTTDHGTIRVNNPIKVIGDKNTNVNLRYKVGKNLSYNKKEVF
                     EITQPAKIGLPSPNVSSAYIFACKDDFFAYPNNYNYYVSYYKNTFQHGGVSLEEMLVP
                     LIVMNPK"
     gene            complement(2837..6085)
                     /locus_tag="PLD44_07790"
     CDS             complement(2837..6085)
                     /locus_tag="PLD44_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOF99267.1"
                     /translation="MTAYFNIYFNGLESYNEGLKNILVAHQDDYSTILPMFPISKHSN
                     ASAATANMDRAIEKSRKAIKLHSIKQKPEKNYNKWNSAEYQAFYNQEEFNPVLKDAWM
                     LIGKAEFHKGDFLGSAGTFSYIARHFASDPDLVAQAKIWLARAYGEMGWLYEAQQVLG
                     DVNQDHLKRQHIGLFASVNADILLKNHQYKEAIPFLRVAIDKETDKKLKQRFLFILAQ
                     LYQKTGDEKAAYETYTKVLQSNPPFEMDFYARIQRSELKAANHAEVQKDLNKMLKSSK
                     YKDYQDQIYYVLGKNFLHRGDTAQAINAFRSSIEKSTRNGYDKANSLITLGDLFYHQK
                     NYKEAYPCYDAASKIIAEEHPDYRRISKLADILSEFVTQYDIVMLQDSLQRLAAMPEK
                     QRMEVIQKIISQVEKEEKAAAKKAEEELLGLNYPNNDDEETLIPIGAQMGNSNDWYFY
                     NQNLMKVGKSEFQKKWGNRKLEDNWRRANKSASLFAEEPNAENVSSEMMMDSTSSTLA
                     ITDNKNPAFYLKQIPLTPLQLKISNEQMAQAMFNVGKIYYEKIQDIPSAIRTFEEFVG
                     RFPDHKLVPDAYFQLYLMESKLGNLANAEKWRNRLVGEFLQTDYSKMLSKPDYIQRLQ
                     RMYQEQDSIYSATYRAYSSNDFATVFRNFQYMQENYPLSELMPKFQFLYALSVGKTQE
                     SQNFEKELDLLVSKYPESDVSTMAKDILALMRQGMVAKSGTTHGSLLAKREEAEKSEI
                     SPSAQQQFSTDTKTKHRLLLITDAPREELNKLMYQIASFNFSRFLIKDFDLGIQPLDS
                     VRNILYVDNFDSYDEVVWYENTMASDIYLTELLSRLKVQELIISEENFALLKNGLSLA
                     DYMAFHDKFLGKNAGSASKQISALPEKEKKSESPKTIAATANKPNSVTETKKTVSTST
                     EKTPLVAPTQPAEQTPNVASVAEQPVKNEPVEKVEVKKEEEVPLFKNLFAYKPNDPHF
                     IAVYVVSGTVDVAKTKAAFDEYNRQNYDVLQLNVRFENVGKQQFFIIGSFTDANTAKS
                     YLLRMVKESKLFEGLKGASYRNLVGTQQNLNVMVQQNALNVYFEFMKQYYLN"
BASE COUNT         1898 a         1336 c         1124 g         2075 t
ORIGIN      
        1 gactggcgat ctccgcgata attttcgtaa acagcttcca atgtacgacg aacacataaa
       61 ttgaacttgt tgttctggtc gtacaaaatg tcgcgccctg ataaagcagc ttctgaaaga
      121 taataaatga gttctttctg ttgcaaagtt agtgtttcga aatcgggaac ccgataacgc
      181 agaatttcaa tatcggcaaa tcggtcaacc gtgtaaggaa atgtttccat cgcgggcgtt
      241 gccggttttt cggaacgaca tcctaaaaaa gctactgtca taagcacaaa taaaaatttt
      301 ttcatacttt tcaaaatttc ttcgtatttc aagttaatag aaaaaatttg attttttcta
      361 atgcaacatt caagttgaaa atgcaacttt ttgattaatc ataaaaataa ggaagcaata
      421 aatcttgaaa attataaaaa ccatcttcct tgtcctttaa attttcggca acaaacaaaa
      481 ctccattacc aaaagcttcc cgcgaaatag actcatgaat cagtctgacg gtctgataag
      541 gaaatccaaa aatgatttcg tgtcggccaa caattccccc tgcacgaatg gaattgattt
      601 ttgctttatc catatccagc acttcagcaa ttttcaaggc agtacctgaa attccatctt
      661 tccccttaaa atgctcttcg actatttcaa tatcaaccca aggggcaatt tttttcaaaa
      721 atttggcaga aatcattaaa tagtttactc caagcgtaat atttggcgac cagaaaacgg
      781 tagtctgttg agccaactta tgcaaaaaag caacttcttt cggtttgtaa tgagaaatag
      841 ccgagataat tttaattttt ctctttgccg ccagctctcc ataagtataa ataccttgac
      901 ctgaagaaaa atcaatgatg acatctacgg gtaaagtatc cattaaattt tcgagattcg
      961 tatttgaagt cgaataaata agtccttccg tactgctggt ttctataccc aagacttcag
     1021 cagcagaacg gtgatcgagt agttttttac gcctgagaat ccatttcagt tcaaaactct
     1081 cgctttgtaa aataactgaa gcaacggctt ttcccgtttt tccaaatcct atcaatccta
     1141 ttctcatagt atttcgtttt ttcatcagag tcttcacctg atgtcgttta taaataaaaa
     1201 tattactgca aattactctt tttcaaccgc tgtcgaaaaa gatttaactt catccgctta
     1261 tttgggattc atcacaatta gaggcaccaa catttcttcc aaagaaacgc cgccatgttg
     1321 aaaggtattt ttataataac tcacgtaata attgtaatta ttcggataag caaaaaaatc
     1381 atccttacag gcaaatatgt aagccgaact gacattgggg cttggtaagc caatttttgc
     1441 aggttgggta atttcgaaaa cttccttttt gttgtagctc aaatttttcc ctaccttata
     1501 gcgcagattg acatttgtat ttttatctcc aatgactttt atgggattat ttactcgaat
     1561 ggtgccgtga tcggttgtaa aaaccacttt gtaaccgcgt tcggctatag ccctgaacaa
     1621 atcgaaagta gcagaatgac ggaaccatga aagggtgagc gagcgataag ctttttcgtc
     1681 gttggccaac tcgcgaacca tttttgattc cgtacgtgca tgcgaaagca tgtcgataaa
     1741 attcagtaca caaacgttca aattattgtt ttccaaatgt ggcaactggt cgatcagatg
     1801 ctgacacgaa acggaatcgt tgattttatg gtacgaaaaa gtatattttt tccgatagcg
     1861 ttgaatttgc gtctgaagca actcactctc cattaggttt ttaccttctt cctcttcttc
     1921 ttccacccaa aattgaggaa acatctgttg aatttgcagc ggcatcagac cggaaaagat
     1981 ggcatttcgc gcatattgcg tagcggtagg aagaatactg caataaagat cttcttcgtc
     2041 aaactgataa taatcgctca acaattcacg tatcattttc cactgatcga ggcggaaatt
     2101 atctaccacc acaaaaaaaa ccttttcgcc attatcaagc aagggaaaaa ctttgttctt
     2161 aaatatttca aaactcatca aaggacgttc ttcggggcgc tcgaaccatc gttcgtagtt
     2221 gttttttatg aattttgaaa aagttgtatt ggcgtccatt ttctgcattc gaagcaattc
     2281 atccatccca ctttcggttt cggaaagttc caattcccag aaaacaagtt ttttataaac
     2341 ttctacccaa tcgtcgtatg tgaacgaatc gttgattttc atgcctattt gtccaaattc
     2401 ggaacgatag gcagctgttg catgttcgtt tacgatttcc tttcgatgaa tatttttctt
     2461 gagcgtcatc agaatctgac ttggattgac aggttttgtc aaataatcgg caattttatt
     2521 gccaatagcc atattcatga tgttttcttc ttcatttttt gtaatcatca ccattggaac
     2581 atccgggtcg atttccttaa tcagtgccag cgtttccagc ccactcaatc ccggcatgtt
     2641 ttcatccaga aaaataatat cgaaattttc ctcctgacag atttctacgg catcattccc
     2701 attggtagcc gtaacaactt catatccctt ttcatccaaa aaaaggagat aaggtcgcag
     2761 catctcgatt tcgtcatcta tccaaagtat tctgtctttt ctcatttcct tcaatttcct
     2821 tttctcaaaa aacttatcag ttcagataat attgtttcat aaactcgaaa tatacgttca
     2881 gcgcattttg ctgtaccata acattcaggt tctgctgcgt tccgaccaga tttctgtaac
     2941 ttgcaccttt taatccttca aacagtttgc tttccttcac catacgcaac aaatacgatt
     3001 tggcagtatt ggcatcggtg aagctgccta taatgaaaaa ttgctgcttg cccacgtttt
     3061 caaatcgcac attcagttgc agcacatcgt aattttgtcg gttgtactcg tcaaaagctg
     3121 ctttggtttt tgccacatct accgttcccg atacaacata aacggcaatg aaatgtggat
     3181 cgttcggttt ataggcaaac agatttttga acagcggaac ttcttcttcc ttcttgactt
     3241 caactttttc aacaggctcg tttttcaccg gttgttcggc aacagaagca acgtttggcg
     3301 tttgttctgc cggttgagtt ggtgcaacca aaggagtttt ttcggtcgaa gtcgaaactg
     3361 ttttttttgt ttcggttacc gaattgggtt tgtttgccgt cgccgcaata gttttgggag
     3421 attcagattt cttttctttt tcaggtaacg cggatatttg tttggaagca gagccggcat
     3481 ttttgcccaa aaatttatca tgaaaagcca tataatccgc taacgataat ccatttttca
     3541 acaaagcaaa attttcttcg gaaattatca attcctgcac tttcaatcgt gaaagcaact
     3601 cagtcaaata aatatccgaa gccattgtat tttcatacca aaccacttcg tcgtaagaat
     3661 cgaagttgtc aacatataat atatttctga cactgtccaa cggttgaatt cccaaatcaa
     3721 aatccttgat gagaaaacgg gaaaagttga aggaagcaat ctgatacatt aacttgttca
     3781 gttcttctcg aggagcatcc gtaatcaaca aaagcctatg tttggttttg gtatctgtag
     3841 aaaattgctg ctgcgcagat ggcgaaattt ccgatttctc cgcctcttcc cttttggcaa
     3901 gcaatgaccc gtgtgtagta ccggattttg ctaccatccc ctgacgcatc agcgccaaaa
     3961 tatctttcgc cattgtactg acatcacttt caggatattt tgaaaccaac agatccagtt
     4021 ctttttcaaa gttttgcgat tcttgtgttt tgcccacact caacgcataa agaaactgaa
     4081 atttaggcat caattccgac aagggataat tttcttgcat atattggaaa tttcggaaaa
     4141 ccgtagcgaa atcattactc gaataagccc gatatgtagc actgtaaatt gaatcctgct
     4201 cttgatacat tcgttgcaaa cgttgaatat aatccggttt tgaaagcatt tttgaatagt
     4261 cggtttgtag aaattcaccc acaagtctgt tgcgccattt ttctgcatta gccagattgc
     4321 cgagtttaga ttccattaaa tacaactgaa aataagcatc cggaacgagc ttgtgatcgg
     4381 gaaatcttcc gacaaattcc tcaaaagtcc gaattgcaga aggaatatcc tgaatctttt
     4441 cataataaat ttttcccaca ttaaacattg cctgcgccat ctgctcattt gaaattttca
     4501 attgaagcgg cgtcagcggt atttgtttca gataaaatgc cggattttta ttgtcggtga
     4561 tggccaatgt tgatgacgtg ctgtccatca tcatttcact gctgacgttt tcggcgttag
     4621 gttcctctgc aaaaagcgaa gccgatttat tggcacgacg ccaattgtct tccaattttc
     4681 ggttgcccca ttttttctga aactcacttt tcccaacttt catcaaattt tgattataaa
     4741 aataccagtc gttggaatta cccatttgag caccaattgg gatgagggtt tcttcatcgt
     4801 cattattggg atagttcaat cccagcaact cttcttcagc ttttttagca gccgcctttt
     4861 cttccttctc aacctgactg ataatttttt gaatcacttc catacgctgt ttttcgggca
     4921 tggcagccaa gcgctgcaaa ctgtcctgca acattactat atcgtattgc gttacaaatt
     4981 cactcaaaat gtcggctaat tttgaaatac gccggtagtc ggggtgttct tcagcaataa
     5041 ttttggaagc agcatcatag caaggatatg cttccttgta attcttctga tgataaaaca
     5101 aatcaccgag cgtaatcaga gaattggctt tatcataacc atttcgggtg cttttttcaa
     5161 tagaggaacg gaaagcattt atggcctgag ccgtgtcgcc ccgatgcaag aaatttttgc
     5221 cgagaacata ataaatctga tcctgataat ctttatattt ggaactttta agcattttgt
     5281 tcaaatcttt ctgcacttct gcatggttgg ctgctttcag ctccgatcgc tgaatgcgtg
     5341 cgtagaaatc catttcgaaa ggcggattgg attgaagcac tttggtatag gtttcgtagg
     5401 ctgctttttc gtcgcccgtt ttctgataaa gctgagccaa aatgaaaagg aaccgttgtt
     5461 tcaatttctt gtcggtttcc ttgtcaattg caaccctcaa aaaagggatc gcttctttgt
     5521 actgatggtt tttcaacaga atatctgcat taacagaagc aaaaagaccg atatgctggc
     5581 gttttaaatg gtcttggttg acatctccca aaacttgctg agcttcgtaa agccaaccca
     5641 tttcaccata agcacgagcc aaccaaattt tggcttgtgc aaccaaatca ggatcagaag
     5701 caaaatggcg ggcgatgtag gaaaatgttc cggccgaacc caaaaaatcg cctttatgaa
     5761 actcggcttt cccaataagc atccatgcat cttttaaaac aggattgaat tcttcctgat
     5821 tataaaaagc ctggtattcg gcagaattcc atttattgta attcttctcg ggtttttgct
     5881 tgatggagtg aagctttatg gcttttcgcg atttttctat ggcacgatcc atatttgccg
     5941 tagcagccga agcattggaa tgtttactaa tgggaaacat cggaagaata gtggaataat
     6001 cgtcctggtg agctaccaaa atgtttttca atccctcgtt gtaactttcc agcccattga
     6061 aatagatatt aaaataagca gtcatcgcat gataattacg agaaaaccat gtgtttttct
     6121 gcattgaaca actgctcaca aaaattatga tcagcagaaa cagtatttta tttaaatttt
     6181 tccttttcaa caattatcgt gaatcaaatt aatcaataat ataagagatt gtttaataaa
     6241 aaatattctc cactaaaaca aaaacaaaaa aattgaaatt ctattatttt acaacttaca
     6301 aacttacata aaatttttaa tttttcacca ttcaacataa attgtgtagc cataaaatca
     6361 tactcatttc agaaaaaaac acacagataa tactttaata caagaaaaaa gctccagagg
     6421 atcaacattt ctg
//
LOCUS       DAOSIB010000097         6421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_10939_length_6421_cov_9.281181, whole genome shotgun sequence.
ACCESSION   DAOSIB010000097 DAOSIB010000000
VERSION     DAOSIB010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10939_length_6421_cov_9.281181"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..524
                     /locus_tag="PLD44_07795"
     CDS             279..524
                     /locus_tag="PLD44_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOF99268.1"
                     /translation="MDIKLKIGQRIKELREKAEMSQKDLAYTADLDRSYIASIENGQR
                     NVSIVNIEKIANALNVTLKEFFNNNEFEKHTKSNRKF"
     gene            484..1311
                     /locus_tag="PLD44_07800"
     CDS             484..1311
                     /locus_tag="PLD44_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99269.1"
                     /translation="MSLKNIQKAIENFKELIETSIIENGAKGKEAMIRSSRPILNIHE
                     AVKVELVEAGINEDLIFPPLNSRTPELKLAGSRKQKNQDVCVVSNLDKAEEILEEGLL
                     QDVVDEYGEKFTERTISINIRSQISSIQKNFDTLYERTTSEAINLHDRCPKMVLGEVY
                     MIAVPEYDDKEVKNNKIVFKKPNQATVLKYIKSFQAINNRKGTEKNFYKYEKVCLLIV
                     DFSQTPAKIYNTSDELKESGLIPDDSTIDISELSWEKFVPDLLEVYKERFSSTDIDN"
     gene            complement(1272..3149)
                     /locus_tag="PLD44_07805"
     CDS             complement(1272..3149)
                     /locus_tag="PLD44_07805"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOF99270.1"
                     /translation="MVKKAILNNLISNQDLTNLEKHLVCQYLTNKNVDFTKSPILTQY
                     LKGFEQDTDLFFGVSALEIETIKDLENHLELIIPKTDRKLNGAFFTPDYIIDFIINEV
                     KPQPTDKNLDPSCGCGAFLIGLTDYYKNTFGKSIKSIIKENIYGSDILAYNIHRTKLL
                     LTIYALQHNEILEETDFNLFNQDSLKAEWNLQFDTVVGNPPYVKFQDLSDENREYLVK
                     NWTTVEGGTFNLYFAFFELGYKLLKPTGRLGFITPNNYFTSLAGEAMRRFFQQKKCVS
                     RIIDFTHKKVFDAQTYTALTFLNKQENEAITFDRLKNGYTPQDFLPIAKGSPNYLKDL
                     NVKKWRLLKTDEQKNIKTIETIGTQISKLFDICVGIATLKDDVFFIDGSRERNGYFIK
                     TTNKGTFEIEKEITKPVYKISDFKTQEEAEQNTRRIICPYTIKGKTATPIPETEFKTR
                     FPKCYEYLLSEKETLLARDKGKVKFEPFFVWGRTQGLAKTGKKILNPTFSQTPRFLLI
                     NEEESYFTNGYGTYFREQETETLFAESINPISKIENIDVVQKIFNSIVMHYYVSKTSV
                     AIEGGYPCYQKNFIEKFTIPEFSESEIELLRSLNDKNEIDNFLIEKYQLSISVEENLS
                     L"
     gene            complement(3313..3801)
                     /locus_tag="PLD44_07810"
     CDS             complement(3313..3801)
                     /locus_tag="PLD44_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOF99271.1"
                     /translation="MLKKIRVTVSVILFTFITLYFLDFAGYLPGQFKVLTEIQFIPAL
                     LALNVVVLVALLVLTLLFGRVYCSSICPMGIFQDVVAWVSKKIRKKKKHTFSKAKNVL
                     RWSVLGITLVAFLFGFTFLLGLLDPYGAHGRIVTHVFRPAYLAGNNLLESIFTSFGNH
                     TL"
     gene            complement(3829..4770)
                     /locus_tag="PLD44_07815"
     CDS             complement(3829..4770)
                     /locus_tag="PLD44_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOF99272.1"
                     /translation="MDRRKFLKAIALTGAATTVIKDADAMSTLSQSFKAEDAAATYDL
                     VAVLGGEPEAMFRKAISELGGMKKFISKGDKVCVKPNIGWDKTPELAANTNPGLVAEI
                     IKQCFDAGASEVTVFDHTCDDWRRCYANSGIEEAAKAAGAKVVPAHQESYYRSVSLPK
                     GKNLKETKVHQAIIDADKWINVPILKNHGGAQLTISMKNYMGIVWDRGYFHANNLQQC
                     IADVCTYDKKPVLNVVDAYRLMKTSGPGGRSLADVVLSKGLFISQDIVAVDTAAASFF
                     NQVREMPLERVGHLKRGEELGIGTMDLEKLNIKRIRM"
     gene            complement(4822..5220)
                     /locus_tag="PLD44_07820"
     CDS             complement(4822..5220)
                     /locus_tag="PLD44_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HOF99273.1"
                     /translation="MNSKSFKEVMQRFREIEFHESFDMIVAIANGGIVPAAVINQRLN
                     IEIQLLKISLRDNHQQKKYDNPKLVAPIDFDFKNKTVLLVEDRVKTGATLQFAMELLD
                     EAKLIKTFAVNGTADYALYNEACFKFPWIL"
     gene            complement(5283..5795)
                     /locus_tag="PLD44_07825"
     CDS             complement(5283..5795)
                     /locus_tag="PLD44_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOF99274.1"
                     /translation="MTTTTTKLYSLSLSDVKTYLFALVFIAGNILFPQLAHLIPQGGL
                     IFLPIYFFTLIGAYKYGIFVGLLTAILSPLTNNLLFGMPPTAALPAIFVKSVVLAIAA
                     AVIAERTGKVSFWGILLAVLAYQVVGTGVEWIMTQSFVVAVQDFRIGFPGILIQLVGG
                     YFVLKALART"
     gene            6095..>6421
                     /locus_tag="PLD44_07830"
     CDS             6095..>6421
                     /locus_tag="PLD44_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOF99275.1"
                     /translation="MTNLFINRDLKDKIISQSRYFPVITITGPRQSGKTTLCKQMFPD
                     YHYVNLEDIAKTEIIKQNPKAFLEIHSKGLIVDDVQQYPDLFSYVQVVVDENPESHIV
                     LTGSSNF"
BASE COUNT         1971 a         1170 c         1196 g         2084 t
ORIGIN      
        1 acgctgtgat ggtggaaaaa acacttaaat tataaatact tacccgataa aacgtttcgg
       61 gtattgccga aggcggggaa ttttaacacc gagcttcaat agaaatacca atgttcaaat
      121 atagcaaaaa gttcaaaatg atttcttcag ccccgctttt ggcaatacct tgttataggc
      181 tgcccctagt tctgtcgttt attgttatta gtcaaaatta ttttcaattg ttacttatag
      241 tctttgattt atagtcaaga tattctcact tttgcaacgt ggatataaaa ctaaaaatag
      301 gtcagcgaat aaaagaattg agagaaaaag ccgaaatgtc tcaaaaagac ttggcttata
      361 ctgctgattt ggatagaagt tatatcgcaa gtattgaaaa tggacagcgt aatgtttcta
      421 ttgtcaatat tgaaaagatt gccaatgcgt taaatgtaac gctcaaagaa ttttttaata
      481 ataatgagtt tgaaaaacat acaaaaagca atagaaaatt ttaaagagtt aatagaaact
      541 tcaataattg agaacggtgc aaaaggaaaa gaagctatga ttaggtcttc tcgtccaatt
      601 ttaaatattc acgaagctgt aaaagttgag ttggttgaag ctgggattaa tgaagattta
      661 atttttccac ctttaaacag ccgaactccc gaattaaaac ttgcaggttc tcgtaaacag
      721 aaaaatcaag atgtttgtgt cgtttcaaat cttgataaag ctgaggaaat attagaagaa
      781 ggattgttgc aagatgttgt ggatgaatat ggcgaaaagt ttacagaaag aacaatttct
      841 ataaatatca gaagtcaaat aagcagtatt cagaaaaact ttgacacgct ttatgaaaga
      901 acaacttcgg aagctattaa tttgcacgac cgttgtccta aaatggtttt aggtgaagtt
      961 tatatgattg ccgtccctga atatgatgat aaagaagtca aaaacaataa aattgttttt
     1021 aaaaagccta atcaagcaac agtattgaaa tacattaaat catttcaggc aataaacaat
     1081 agaaaaggaa cagagaagaa tttttataaa tacgaaaaag tttgtttgct tattgttgat
     1141 ttcagtcaaa ctccagccaa aatttataat acaagtgatg aactgaaaga atcaggactt
     1201 attcccgatg attcaacaat tgatattagt gaactttctt gggaaaaatt tgtaccagat
     1261 ttgttggaag tttataaaga aagattttct tccacagata ttgataattg atatttttca
     1321 attaagaaat tgtcaatttc atttttatca tttagagagc gaagcaattc aatttcactt
     1381 tccgaaaatt caggaattgt aaatttttca atgaagtttt tttgataaca aggataacca
     1441 ccttcaatag caacacttgt tttgctaacg taatagtgca ttacgattga attgaaaatt
     1501 ttttgaacta catcaatatt ttcaattttt gaaatggggt ttatgctttc tgcaaataaa
     1561 gtttcggttt cttgttctct gaaatatgtg ccgtagccgt ttgtaaaata ggattcttct
     1621 tcatttatca ataaaaatct tggggtttgg ctgaaagttg ggtttaaaat tttctttcct
     1681 gtttttgcta aaccttgtgt tcttccccaa acaaaaaaag gctcaaattt cactttccct
     1741 ttatctcttg ccaaaagtgt ttctttttcg gaaagtaaat attcgtagca tttcggaaaa
     1801 cgcgttttaa attccgtttc gggaattggt gtcgctgttt ttccctttat ggtgtaagga
     1861 caaataattc ttcttgtatt ttgttcggct tcttcctgtg ttttgaaatc tgaaattttg
     1921 taaacaggtt tggttatttc tttttcgatt tcaaaagttc ctttattggt agttttgatg
     1981 aaataaccgt ttctttctct gcttccgtca ataaaaaaca catcatcctt taatgtagca
     2041 atgcctacgc aaatgtcaaa caatttgcta atttgcgttc cgattgtttc aattgtttta
     2101 atgttttttt gttcgtcagt tttcagcaaa cgccattttt ttacgttcaa gtctttcaga
     2161 taatttggcg aaccttttgc tattggcaaa aagtcttgtg gtgtgtagcc gtttttaagt
     2221 cggtcaaacg taatagcttc attttcctgt ttgttgagaa aggtcaatgc tgtgtaagtt
     2281 tgagcatcaa aaactttctt atgcgtaaag tcaataatac gtgaaacgca ttttttctgt
     2341 tgaaaaaatc tacgcattgc ttcgcctgca agcgaagtaa aataattgtt tggtgtaatg
     2401 aaacctaacc gtcctgtcgg tttcagtaat ttatagccta attcaaaaaa agcaaaataa
     2461 agattgaacg ttccgccttc aacagttgtc caattcttta ctaaatactc cctgttttca
     2521 tcagaaaggt cttgaaattt cacataaggt ggattgccaa caactgtgtc aaactgcaaa
     2581 ttccattcag cttttaaact gtcttgattg aataagttga agtctgtttc ttctaaaatc
     2641 tcattgtgct gtaaagcata aattgtcagc aaaagtttgg tgcgatgaat attgtaagca
     2701 agaatatccg aaccgtaaat attttctttt atgattgatt ttatagactt tccaaaagtg
     2761 tttttatagt aatcggtcaa gccaattaaa aatgctccac aaccgcaact tggatcaaga
     2821 tttttgtcgg ttggctgtgg tttaacttcg ttaatgataa agtcaataat atagtctggc
     2881 gtaaaaaatg caccatttag ttttctgtcc gtttttggaa taataagctc taaatgattt
     2941 tcaaggtctt ttattgtttc aatttccaaa gcagaaactc caaaaaacaa atcggtgtcc
     3001 tgttcaaaac ctttgaggta ctgtgtcaat attggacttt tggtaaagtc aacattttta
     3061 ttggtcagat attgacaaac caaatgcttt tcaagatttg taaggtcttg attagaaata
     3121 agattgttca atatcgcttt ctttaccatt tttcttgctg actataagca acaaagttag
     3181 tgaaaatttt actttgggca aatagttttt agaaattgtt ttgaacggtc aaaaaaaaga
     3241 gtgggtgggt ggacttggat tattagtttt gtttgtccgt tgattgttgc acggtcgctc
     3301 tgtaggggtt gcctataacg tgtggttccc gaacgatgtg aaaatgcttt ccagcaaatt
     3361 gttccctgcc agataagcag gacggaaaac atgcgtaaca attcgtccgt gcgctccgta
     3421 aggatcaagt agtccaagca ggaatgtaaa cccaaacaag aaagccacaa gcgttattcc
     3481 cagcacactc cagcggagta catttttagc cttactgaaa gtgtgttttt tctttttcct
     3541 gatttttttt gaaacccacg ccacaacatc ctgaaagata cccatgggac agatggatga
     3601 gcaataaaca cgtccgaaca gcagcgtgag cacaagcaac gcaaccagta caactacatt
     3661 gagagcaagc agggcaggaa taaattgtat ttcggtcaat accttaaact gcccgggaag
     3721 atagcccgcg aagtcgagaa aataaagcgt gatgaacgtg aaaagaataa cggagaccgt
     3781 tacgcgtatt tttttcagca tatggtatat ccccaattaa atttgaattt acatgcgtat
     3841 tcttttgatg ttcagttttt cgaggtccat tgttcctata cccaactcct ctcctctttt
     3901 cagatgccct accctttcaa gcggcatttc cctgacctgg ttaaaaaaac tggctgctgc
     3961 cgtatctacc gctacaatgt cttgagaaat aaaaagtcct ttcgagagta ctacgtcggc
     4021 aagtgaccta ccccccggtc cgcttgtttt catcaatcgg tatgcatcta ccacattgag
     4081 taccggtttt ttgtcatagg tacacacatc ggcgatgcat tgctgcaaat tgttggcatg
     4141 gaaataacct ctgtcccaca caatacccat ataattcttc atggaaatgg ttaattgagc
     4201 accaccgtga tttttaagga taggcacatt gatccatttg tcagcatcta taatggcttg
     4261 atgcaccttg gtctctttca gatttttgcc ctttggtaaa gaaacgctcc ggtaatacga
     4321 ttcctggtgt gcaggaacca ctttcgcacc cgcagccttg gcggcttctt caattccgct
     4381 gttggcgtag cacctgcgcc agtcgtcgca agtatggtca aatacggtta cttccgatgc
     4441 acctgcatca aaacattgct taataatttc cgctaccaac ccgggattgg tattggctgc
     4501 caattccggt gttttatccc agccaatatt aggttttaca catactttat cgcctttgct
     4561 gatgaatttt ttcattcctc ccagctcgga aatggctttt cggaacatgg cttcaggctc
     4621 gcctcccaat acggcaacca agtcgtatgt tgctgccgcg tcctctgctt taaacgattg
     4681 tgagagcgta ctcatcgcat cagcatcttt aatgactgta gtagccgctc cggttaatgc
     4741 tattgctttt aagaattttc gtctgtccat attgtttatt ttttagaaga tattgatatt
     4801 cagttgaatt tacgatttgc tttaaagtat ccagggaaat ttaaagcacg cttcgttgta
     4861 aagcgcataa tcagcagttc catttactgc aaaagttttt atcagtttgg cttcgtctaa
     4921 aagttccatg gcaaattgca gcgtagctcc ggtttttaca cgatcttcca ccaacagcac
     4981 agttttgttt ttaaaatcaa aatcaatggg cgctacaagt ttcggattgt cgtatttctt
     5041 ttgctgatgg ttatcgcgca agcttatttt aagaagttgg atttctatat tcaaccgttg
     5101 gttgatgacc gctgcaggaa caattccccc attggcaatg gctacaatca tatcaaagct
     5161 ctcgtgaaac tcaatctcac gaaaccgctg cattacctcc ttaaaagact tgctattcat
     5221 tgtcaacaga atacatctcg ccgcaactac tgcctatctt taccagttgc taattatcaa
     5281 tctcaggttc tcgccagggc tttcaatacg aaatatccgc caaccaattg gatcagtatg
     5341 ccggggaatc cgatgcggaa atcctgaaca gccactacaa agctttgtgt cattatccat
     5401 tcaacacctg ttcccactac ctgataagcc aggacagcca gcaatatacc ccagaaagaa
     5461 acttttccgg tacgttctgc tattactgcc gcggcaatgg ctaaaacaac cgatttaacg
     5521 aagattgcag gaagcgctgc cgtagggggc atcccgaaaa gcaggttgtt cgtcaggggc
     5581 gacaaaatag cggtaagcaa tcccacaaaa atgccgtatt tgtaggcgcc aataagcgta
     5641 aaaaaataga taggcagaaa gatcaatccg ccttgcggaa taagatgtgc taattgcgga
     5701 aacaatatat tacccgcaat aaacaccaaa gcgaacaaat aagtttttac atcgcttaaa
     5761 ctgagtgaat aaagttttgt tgttgtcgtt gtcataatat gtgataattt tgtttagctg
     5821 tttattttaa tattcaggtc gaagcacaaa actacataaa aatacgggta aaagaataaa
     5881 agtgggtgtt tttttgtgaa aaattacagg gaggatggat tcaagggtgt taaagtatag
     5941 tcctttcggg tattttggtg tatggaacct ttgatgatta tgagtagaga atataagcta
     6001 tcaatcatct tttcatttga taatttgttt ggaatttata ttctttttat atttgcggac
     6061 agatttaaag ttcaacttaa aatttgtccg aactatgact aacttattca taaacagaga
     6121 tttaaaagac aaaataataa gtcaatcgcg ctattttccg gtaataacca ttaccggtcc
     6181 cagacaatcg ggcaaaacaa ccttgtgtaa gcagatgttt cccgattatc attatgtgaa
     6241 tttggaagat attgcaaaaa cggaaatcat caagcaaaat cccaaagcat ttttagaaat
     6301 tcactcgaag ggtttgatcg tggatgatgt tcagcaatat cccgatctat tttcgtatgt
     6361 tcaggtggtg gtcgacgaaa accctgagtc gcatattgtt cttaccggaa gtagtaattt
     6421 t
//
LOCUS       DAOSIB010000098         6420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_10943_length_6420_cov_8.419324, whole genome shotgun sequence.
ACCESSION   DAOSIB010000098 DAOSIB010000000
VERSION     DAOSIB010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10943_length_6420_cov_8.419324"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /gene="csm5"
                     /locus_tag="PLD44_07835"
     CDS             complement(<1..656)
                     /gene="csm5"
                     /locus_tag="PLD44_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HOF99276.1"
                     /translation="MSNIQLTTLTPVHVGSGELLQYNADFVEDKDGSDSYIYVVEPAK
                     ILQLIGMENIDRWISIIENAGNTKEFVQKFSPIKSPEAFSSRKILNFAKKIQKGQILN
                     ETIHNGFGFPYIPGSSLKGAIRTAVLASLVGRVNDKEEKILSKDRKGKTIISDKKIQN
                     ELFGNDAKNDLFRFVEVGDAYFDKGSEAAFRMIHLNITNKNSLLDDSKSQIVETIDQE
                     "
     gene            complement(653..1636)
                     /gene="csm4"
                     /locus_tag="PLD44_07840"
     CDS             complement(653..1636)
                     /gene="csm4"
                     /locus_tag="PLD44_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm4"
                     /protein_id="HOF99277.1"
                     /translation="MSEYKIIKFTQFTPLHVGTGKEDYDSSAGMLQSDTLSAALAAIR
                     AQMGKTDDVLEFMQSFTISSAFPYWKELYFMPAMKGELQVAVKGKEEYEYRKWLKKIK
                     FIEIGLWNELVSGKSIVVEQQQLQNEFLIPKISFQKVIKNQVNQRVQVPRIDGTKNKL
                     FSFDWQFFDKEAGLYGLIDADEKVFAEVKELFKLLGETGIGTDKNVGGGKFVPECGAI
                     QIPEIPDANATLLLSLFLPEEAELSQLKLEDAVFELILRGGFLAGSTEEKFRHLRKKS
                     VYMFNAGSLFPTVQEIKGKIVDLSPAWNDEKMHPAYRSGKPFCINIKVPSL"
     gene            complement(1648..2283)
                     /gene="csm3"
                     /locus_tag="PLD44_07845"
     CDS             complement(1648..2283)
                     /gene="csm3"
                     /locus_tag="PLD44_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009012138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HOF99278.1"
                     /translation="MSTIKLNKKINYTGTIVLKTGLHIGGTNAALNIGGPDNFVVRNP
                     LTNVPYIPGSSLKGKMRALIEISTGDVSSKGEASENPNGVAGKLFGVGANKGGHPSRL
                     IVRDAELIIDDPEVDFSRTDLLYAESKTEVTINRVTAEANPRTFERVPAGAKFNLNMV
                     LNIFEGENEDELKRTLEKAMQLLENDYLGGQGSRGYGQVKFENIEITESKY"
     gene            complement(2340..2807)
                     /gene="csm2"
                     /locus_tag="PLD44_07850"
     CDS             complement(2340..2807)
                     /gene="csm2"
                     /locus_tag="PLD44_07850"
                     /inference="COORDINATES: protein motif:HMM:NF015695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HOF99279.1"
                     /translation="MNNYQKNPQQGGNWPQKNSQNYDQPKETPEEFLNHHAIDSKWIT
                     QNTDKSMVDFAEEAGKYMAKQKLTKSKIRSIYGEIKRIQINGFDKEKASFYLLRPKMA
                     YALGRDSSNYGLKLFKLIFDKSSELVQNSNQYENFCNLFEAILAYHKAFGGKD"
     gene            complement(2856..5387)
                     /gene="cas10"
                     /locus_tag="PLD44_07855"
     CDS             complement(2856..5387)
                     /gene="cas10"
                     /locus_tag="PLD44_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Cas10/Csm1"
                     /protein_id="HOF99280.1"
                     /translation="MDFEREQVYLAALLHDIGKFYQRADTGSVAGSKYLKEYSKIEST
                     FLPLSVNGKYSHKHCLWTAQFIEDFQEVFRKLVGADSANLTDKNNLMFLAAAHHLKNE
                     QLSPLGQIIKKADCLSSGMDRNSLEALEDDQDESKWNSFKTKRMVSIFDILNKNKEEL
                     KQQKNWHHLPIDALNLSKNYFPRSEFNENPDYEKLWENFLIEFKFIQANTYRAFSETL
                     LNLLFKYTSCISSSTIVFPDVSLYDHSKTTAALAVCLYDYQHGTAASKDPFLLIGGDM
                     SGIQMYIYQIVSKYAGKNLKGRSFYIQILSDAVVRFLLKKLHLFRANIIYDSGGSFYL
                     IAPNTTFVNQQLTDAILFIEQQMFEKHGIQLYMAIDAVTVSEDALMHKGEEHLGDIWN
                     KLFEKRNKKKNNKYLSLITNSYDKLFTPFMQGGLTKRDRISGEEFFPNEKPFYSPEIS
                     EEFNEENPIKEITGKQILMGKYLRETDFLVVSEQKIPYWDDKIFIAPIELGFYYYFLK
                     TAEIEKMKERLQASADKITLVTLNGKNGNCDFMGDIKIRGLNNIYELKFYGGNEFNGK
                     TFDKMCDSESLSRLGILRMDVDNLGNIFQTGILPEMSTLSRFSALSRSFDFYFSGYLN
                     MLQQEIAPDSSFIIYSGGDDLFIVGDWRATIELAKKIKADFKEFSCHNPSLHISGGIA
                     IVEPTFPIMKSAEESQKEEKAAKEHTCKEAVKNSISFMDMPLHWEKEFPTVELLKNTF
                     VEIIKKGSPKSLLGKIMQHTANADIRNHKVHRNKTYWMLTYDLSRMKERNKDHSLLND
                     LIDNCLKEVCGNVQTSLNGLSITTEYHPLELWAFAARWAELELRS"
     gene            complement(5392..6054)
                     /locus_tag="PLD44_07860"
     CDS             complement(5392..6054)
                     /locus_tag="PLD44_07860"
                     /inference="COORDINATES: protein motif:HMM:NF036560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas6"
                     /protein_id="HOF99281.1"
                     /translation="MLIRFENSIQQQEIEYFRGAVIHLIPSSEILFHNHLQDGFRFSY
                     PLIQYKRIANKAAIMCFNEGTESIGSLLANHNHDIQIGDRIEKLEIAKVQAFDYQIQI
                     WEPMFRYHIRKWLPFNQKNFEDYNRLESIAEKCLFLENILTGNLLSFAKGLHIFFDKQ
                     VVCKILQLENSTLTTYKGIKMNQFDAVFSCNVSIPDFTGLGKGVSIGYGMVTKYKNNT
                     KN"
BASE COUNT         1919 a         1247 c         1055 g         2199 t
ORIGIN      
        1 ttttcctgat caatggtttc aacgatttga gatttactgt cgtccagcag tgagtttttg
       61 ttggtaatat tcaaatgaat catccgaaag gcagcttccg aacctttatc gaaataagca
      121 tcacccactt ccacaaatcg gaacaaatcg tttttggcat cattgccaaa caattcattt
      181 tgaatttttt tatcggaaat aattgttttt ccttttctgt ccttcgaaag tattttttcc
      241 tctttatcat ttacccttcc taccagcgaa gcaagcactg ctgtacgtat ggcgcctttc
      301 aaagaactgc ccggaatata gggaaatcca aaaccgttgt gaatggtttc attcaaaatt
      361 tgtcctttct gaattttttt ggcaaaattc aaaatttttc gagaagaaaa tgcttccgga
      421 cttttgatgg gtgaaaattt ctgaacaaat tctttggtgt ttccggcatt ttcaattatc
      481 gaaatccagc ggtctatatt ttccatgcca atcaattgga gaattttagc aggttctact
      541 acataaatat aggaatccga tccatcctta tcttccacaa aatcggcatt gtattgcaat
      601 aactctcccg aacctacgtg gacaggtgtc aaagtcgtta attgaatatt actcataatg
      661 atggtacttt aatgttgata caaaatggtt ttccgcttcg ataagcagga tgcatttttt
      721 catcattcca tgctggcgat aaatccacaa tttttccttt tatttcttga acggttggga
      781 ataatgaacc tgcattaaac atataaaccg attttttcct caagtgacga aatttttctt
      841 cggtactacc agccaaaaat cctccccgta aaatcaattc aaagacggca tcttccagtt
      901 tcaactgaga gagttcggct tcttccggta aaaaaagcga caacaaaagt gttgcattcg
      961 catccggaat ttcaggaatt tgaattgctc cgcattccgg aacaaatttt cctccgccta
     1021 cgtttttatc ggtacctatt cctgtttcgc ccaacagttt gaaaagttct ttcacttcgg
     1081 caaacacctt ctcatcagca tctatcaagc cgtaaagtcc ggcttctttg tcaaaaaatt
     1141 gccaatcaaa agaaaaaagt ttgttcttcg ttccatctat tcgtggaact tgtacccttt
     1201 gattcacttg atttttgatg actttttgaa aagaaatttt tggaatcaaa aattcgtttt
     1261 gcaactgttg ctgctcaaca acaatgcttt ttccggaaac caattcgttc cacaagccta
     1321 tttcgataaa tttaattttt ttcagccatt ttcgatattc atattcttct tttcctttta
     1381 ctgctacttg aagttctcct ttcattgctg gcatgaaata gagctctttc caataaggaa
     1441 aagcagaact gatggtaaac gactgcataa attccagcac atcgtccgtt ttacccattt
     1501 gggcccgaat tgcagcaagg gcagccgaca aagtgtcgct ttgaagcatt cctgctgagg
     1561 aatcgtaatc ttctttcccc gttcctacat gcaaaggcgt aaattgggtg aattttatta
     1621 ttttatattc cgacatatta ctgaaattta gtacttcgat tctgtaattt caatattttc
     1681 aaatttaacc tgcccatagc cccgtgaccc ttgtccgccc aaatagtcat tttcgagtaa
     1741 ttgcatggct ttctccaaag ttcttttcaa ttcatcttcg ttttcacctt caaaaatatt
     1801 gagtaccatg ttcagattga atttggcccc tgccggcaca cgttcaaaag tacgaggatt
     1861 ggcttcagcg gttactcgat tgatagttac ttccgttttg ctttcggcat agagtaaatc
     1921 cgttctgctg aaatcgactt cagggtcatc aattatcagt tctgcatctc tcacgatcaa
     1981 acgggaagga tgaccacctt tatttgcacc aacaccaaac agctttccgg caactccatt
     2041 gggattttcc gacgcttccc ctttggaact tacatctcct gttgaaattt caatcaaagc
     2101 tctcattttt cccttcagcg agcttcccgg aatataggga acgttggtta atggatttct
     2161 tactacaaag ttatcgggtc cgcctatatt caaagctgca tttgtcccac caatatgcaa
     2221 tccggttttc aaaacgatag taccggtata attgattttt ttgtttaatt ttattgttga
     2281 catgattttt agatttttaa tgaaattgaa agtgtttttt aataaattct aatctacttt
     2341 taatctttac ctccaaaagc tttgtggtaa gccagaatgg cttcaaacag attgcaaaaa
     2401 ttctcgtatt gattgctatt ttgaaccaac tctgaagatt tgtcaaaaat caatttaaaa
     2461 agtttcagtc cgtagttact actgtcacga ccaagagcgt aggccatttt tggacgaagt
     2521 aaataaaaag atgctttttc tttatcgaaa ccattaatct gaatgcgttt gatttccccg
     2581 taaatacttc tgattttcga tttggttaat ttttgtttgg ccatatattt tcctgcttct
     2641 tctgcaaaat ccaccatcga cttatccgta ttttgggtta tccatttcga atcgatagca
     2701 tgatgattta aaaattcttc cggcgtctct tttggctggt catagttttg actattcttt
     2761 tgtggccagt ttcctccctg ttgaggattc ttttgataat tgttcataat ttattgattt
     2821 taagagtttt atattaatgt aaattacttt ttaattcagg aacgcagttc caattccgcc
     2881 caacgggcag caaaagccca gagctccaac ggatgatatt cagtagtgat agaaagtcca
     2941 ttgagtgaag tttgcacatt gccacacact tctttcaggc agttgtcaat caaatcgttc
     3001 aataacgaat gatccttgtt tcgttctttc attcgactca aatcgtatgt aagcatccaa
     3061 tatgtcttgt tcctatgcac tttatgattt ctgatatcgg cattagctgt atgctgcatg
     3121 attttcccca acaacgattt aggagaaccc ttttttataa tttccacaaa tgtatttttc
     3181 aacaattcaa cagtaggaaa ctctttttcc caatgtaagg gcatatccat aaaagaaata
     3241 gaatttttca ctgcctcttt gcacgtatgt tctttggctg ctttttcttc cttctgactt
     3301 tcttcagctg atttcatgat cgggaaggtt ggctctacaa tggcaattcc tcccgaaata
     3361 tgtaaggacg gattgtggca ggaaaattct ttaaaatcgg cttttatttt ctttgctaat
     3421 tcaatagttg cccgccagtc accaacaata aacaagtcgt ctccaccact gtaaatgata
     3481 aatgacgaat cgggagcaat ctcttgctga agcatattga gataacccga aaagtaaaaa
     3541 tcaaaggaac ggcttaaagc ggagaaacgc gacaaggtgg acatttcggg taaaataccg
     3601 gtttgaaata tattgcccag attgtccaca tccattcgca aaattccgag acgagacaat
     3661 gattcactat cacacatttt gtcaaatgtt tttccgttaa attcattacc tccataaaat
     3721 ttaagctcat aaatattatt taatcctcga atttttatat ctcccataaa atcgcaattg
     3781 ccatttttac cgttcaaagt aacgagggta attttatcgg cagaagcttg caatctttcc
     3841 ttcatttttt cgatttcagc tgtcttcaaa aaataataat aaaatccaag ttcaatcgga
     3901 gcaataaaaa ttttatcatc ccagtaagga attttctgtt cggaaacgac caaaaaatct
     3961 gtttcccgca agtattttcc cattaatatc tgtttaccgg taatttcctt aattgggttt
     4021 tcttcattaa attcctcact tatctctggc gaatagaaag gtttttcatt gggaaaaaat
     4081 tcttcccccg aaatacggtc gcgcttggtt aatccacctt gcataaaggg agtaaacaat
     4141 ttgtcgtaac tatttgtgat gagcgataag tatttattat ttttcttttt gttacgtttc
     4201 tcgaaaagtt tattccatat atcgcccaaa tgctcttctc ctttatgcat taaagcatct
     4261 tcagaaaccg ttactgcatc aattgccata taaagttgaa taccatgttt ttcgaacatt
     4321 tgctgttcaa taaacagaat ggcatctgtg agttgttggt ttacaaatgt agtatttgga
     4381 gcaatcagat aaaaacttcc acctgaatca taaatgatgt tagcacgaaa gagatgaagc
     4441 tttttcaata aaaaacgtac cactgcatcg gaaaggattt gaatgtaaaa agatctccct
     4501 tttagatttt tgccagcata tttcgaaaca atttgataaa tatacatttg aataccactc
     4561 atatcacctc cgatcaggag aaaaggatct ttgcttgcgg cggttccatg ttgataatca
     4621 taaagacaaa ctgctaatgc tgcagtggtt tttgaatggt cgtaaaggga gacatccgga
     4681 aagacaatcg tactcgaaga aatgcaagaa gtgtatttaa acaacagatt caacaatgtt
     4741 tcgctaaaag cacgataggt atttgcctga ataaatttga actctatcag aaaattttcc
     4801 cacaattttt cgtaatcggg attttcatta aattcgcttc ttggaaaata attttttgat
     4861 agattaagtg cgtcaatggg taaatgatgc cagttttttt gttgttttaa ttcttcctta
     4921 ttcttattca aaatatcaaa aatagaaacc attcgttttg ttttgaaaga attccacttt
     4981 gattcatcct gatcatcttc taacgcttcg aggctattcc tatccattcc agacgagaga
     5041 caatcggctt ttttaatgat ttgccctaac ggtgaaagct gttcattttt caaatgatga
     5101 gcagcagcca aaaacatcaa attatttttg tcggtgagat tggcggaatc ggcaccgaca
     5161 agttttctga aaacttcttg aaaatcttca ataaactgag ctgtccacaa acaatgcttg
     5221 tgggaatact tcccattcac tgaaagcggt aggaaagtac tttcaatctt actatactct
     5281 ttcaggtatt tgctgccagc aacgctgccc gtatcagcac gctggtaaaa tttaccaata
     5341 tcgtgcagta aagcagccag ataaacttgt tccctttcaa aatccataga cttaattttt
     5401 tgtgttgttt ttatattttg taaccatacc gtaaccaata ctaacaccct taccaagacc
     5461 agtaaaatcg ggaatagaaa cattacagga aaacacagcg tcaaactggt tcatctttat
     5521 tcctttgtaa gtggtaagtg tcgaattctc caattgcaaa attttacaaa cgacttgctt
     5581 gtcgaaaaaa atatgaagac cttttgcaaa agataaaaga ttgcctgtca aaatattctc
     5641 gagaaaaaga catttttcgg ctatactttc caatcgattg taatcctcaa aatttttttg
     5701 attgaacggc aaccatttac gaatgtgata ccgaaacatt ggttcccata tctgaatttg
     5761 ataatcgaaa gcttgtactt ttgctatttc aagcttttcg atacgatcgc caatttgaat
     5821 atcgtgatta tgatttgcca acagagagcc aatggattcc gtgccttcgt taaaacacat
     5881 gattgctgct ttgttggcaa ttctcttata ttgaatcagt ggataagaaa aacgaaagcc
     5941 atcttgaaga tgattgtgaa aaagaatttc ggacgatggg attaaatgaa tcacagcacc
     6001 tcgaaaatat tcaatttctt gttgctgaat gctgttttca aacctaatta acattaatcg
     6061 agtgtttttc atgttattag taattaaaaa aggttgaatt ctgaaagaat aattcagaaa
     6121 aaattgactt gtagaatttt aattggtaca aatttataaa atttaataag aattatcatg
     6181 tttaaagtca attaattttt tgttttttta atcaacaatt aaaacctgtt tgtcagcttg
     6241 ataatgagaa acttttttct gataagcata actttcacac catcattctt acaggcttgg
     6301 gattttgtga agggaatgaa tattttttat aattttgcca aagggatagt ttataaagtc
     6361 tgaacttttg attagtgata aagatgaata ggagcaatga atttaccgaa ttttcgcata
//
LOCUS       DAOSIB010000099         6272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_11275_length_6272_cov_10.805212, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000099 DAOSIB010000000
VERSION     DAOSIB010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11275_length_6272_cov_10.805212"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..1515)
                     /gene="ffh"
                     /locus_tag="PLD44_07865"
     CDS             complement(196..1515)
                     /gene="ffh"
                     /locus_tag="PLD44_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005652735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOF99282.1"
                     /translation="MFESLSERLERSFKILKGEGRITEINVAETLKDVRKALLDADVN
                     YKTAKTFTDTVKEKALGQNVLTSIKPSQLMIKIVHDELAQLMGGSSVDINLKGSPAVI
                     LMAGLQGSGKTTFSAKLANLLKTKRGKNPLLVAGDVYRPAAIEQLKVLGEQLKIPVYS
                     EENNKNPVSIAEAAIKYAKLHGYDVVIVDTAGRLAVDETMMNEIAAIKKAIQPQEILF
                     VVDAMTGQDAVNTAKEFNDRLNFDGVVLTKLDGDTRGGAALSIRSVVDKPIKFVGTGE
                     KMDALDVFHPERMADRILGMGDIVSLVERAQEQYNEEEARRLQKKIAKNQFDFNDFLS
                     QISQIKKMGNIKDLASMIPGMGKALKDVDIDDSAFTGIEAIIFSMTPEERENPKLLNG
                     SRKLRIAKGSGRSIVEVNRLLKQFDQVGKMMHMATVGQGKLKMKKRK"
     gene            complement(1560..3554)
                     /locus_tag="PLD44_07870"
     CDS             complement(1560..3554)
                     /locus_tag="PLD44_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOF99283.1"
                     /translation="MEEDKITGLPENAHRELKEGEEYKPILPAEKVFPEVNFRSVSTG
                     LLMAVLFSAAAAYLGLKVGQVFEAAIPIAIIAVGLSGAAKRKNALGENVIIQSIGASS
                     GVIVAGAIFTLPALYILQAKYPDISVNFMQVFLSSLLGGVLGILFLIPFRKYFVSDMH
                     GKYPFPEATATTQVLVSGEKGGDQAKPLIVAGIIGGLYDFIVATFGAWAEVFTSRVLP
                     FGAQIAEKAKLVFKVNVGAAVLGLGYIVGLKYAAIICAGSALIWFVVVPVLPLLNDQV
                     IHLLNPAFQGTLAVMSPEIIFRDFARHIGIGGIAMAGIIGVAKSWKIIMDAVGLAAKE
                     LKGGAKSLEQQKIKRTQRDISMKIIIFGIIGALLATFLFFYFGVVGNLTHAVVGILVI
                     GIIAFLFTTVAANAIAIVGTNPVSGMTLMTLILSSVIMVAVGLKGTAGMTASLIMGGV
                     VCTALSMAGGFITDLKIGYWIGTTPAKQESWKFLGTLVSAATVGGVIMILNKTYGFTG
                     ENALVAPQANAMAAVIEPLMSGVGAPWLLYGMGALIALVLNFFNVPALAFALGMFIPL
                     ELNVPLLVGGLVSWYVSGRSSNKELNRLRQEKGTLLASGFIAGGALMGVVSAALRFGG
                     IQLTNESWLQQPGSEWLALFMYVLLILYFVWDSMRIKDNK"
     gene            complement(3613..5589)
                     /gene="pulA"
                     /locus_tag="PLD44_07875"
     CDS             complement(3613..5589)
                     /gene="pulA"
                     /locus_tag="PLD44_07875"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HOF99284.1"
                     /translation="MMLVTGWFCSCQKTPKYASYDDYPVYDGSDLELTYTSEASTFRV
                     WAPTAAEVKLLLYDDGIDDGASRVVNMKRSENGTWKLRIKEDLKGKFYTFQVKVNDRW
                     LDETPGMWVKATGVNGKRAAIIDMIETNPAGWEMDVRPPLKDEADIILYEVHVRDFSM
                     SPNSGMKNKGKFLAFTEHGTKNTMGQATGIDHLKELGVTHVHLLPSFDFASIDETKLN
                     ENHYNWGYDPLNYNVPEGSYSTDPYDPAVRIKEFKQMVQALHQSGIRVVMDVVYNHTF
                     TGENSHLNLLVPGYFYRMNPDGTWSNASGCGNETASERAMMRKFIVESVVYWATEYHI
                     DGFRFDLMGVHDIETMNAVRLALDKVDPTICIYGEGWTAGPSVYPEEKRALKKNAKQL
                     DRIAVFSDDIRDALKGSWQHPEIPGFVSGVDSLEESVKFGVVGSIQHPQIDYSRLIYS
                     KEPYVNDPTQVISYVSCHDDLCLADKLRSSKPLGATNDEILRFDKLAQTIVFTSQGIP
                     FIYAGEELFRTKKGVHNSYQSPDSINQINWDNKTVFQDLYQYYKGLIALRKAHPAFRL
                     GTQENVQQYLHFLDWGIPNVVAFTLTDNAKGDTWRNILVIYNGNRKSVVVEIPEGEWN
                     LVCHDGRINLTGIAQVKTTAFVVAPSSASILYQQ"
     gene            complement(5634..>6272)
                     /locus_tag="PLD44_07880"
     CDS             complement(5634..>6272)
                     /locus_tag="PLD44_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOF99285.1"
                     /translation="EFGHSLHGMLSDCTYPSLSGTNVYRDFVELPSQIMENWASQKEF
                     LDQFAFHYQTGEKIPVELIQKIKDAENFNVGYSCLRQLGFGYLDMAWHTLSSPFEGDV
                     VDFEKQAVAKTQILPYVENTAISPTFNHIFSGGYAAGYYSYKWAEVLDADAFSVFEKN
                     GIFDQATAQSFMENILKKGGTEHPMILYKRFRGQEPSIDALLRRNGIISTNN"
BASE COUNT         1848 a         1462 c         1143 g         1819 t
ORIGIN      
        1 tcagaatata atttattttt taaaggtctg atggaaccca catgtctgaa tattcgtgtt
       61 tgtgtatgca atacatgtgg gttttttaaa aatttcccag atcagatgac aaataaaaaa
      121 attaaaacag acaacacggt ttaggtgcaa gtttgagttt aactgataaa atcaacaaaa
      181 tgtaaactat aatcgttatt ttcttttctt cattttcaat tttccctgcc ctactgttgc
      241 catgtgcatc attttaccta cttggtcgaa ttgtttcaac aatcggttta cttccacaat
      301 acttcttcca ctacctttgg caatgcgcaa ttttctgctt ccgttcagca atttcgggtt
      361 ttcgcgttct tcaggtgtca tggagaaaat tatggcttca ataccggtaa atgcgctatc
      421 gtcaatgtcc acatctttca aggcttttcc catgccggga atcatggaag ccaaatcctt
      481 tatgtttccc atctttttga tttgggaaat ttgggacaga aaatcgttaa aatcaaattg
      541 atttttggca atttttttct gtaatcttcg ggcttcttct tcattatact gttcctgagc
      601 tctttctacc aacgatacaa tatctcccat gcccaaaata cggtcagcca ttcgttcggg
      661 atgaaacaca tccaaagcat ccatcttttc acctgttcca acaaatttaa tgggcttgtc
      721 gacaaccgag cgaatggaaa gtgccgctcc gccacgtgta tcgccatcca atttggtcag
      781 caccacacca tcaaaattga gtcgatcgtt gaattccttt gccgtattca cagcatcctg
      841 acccgtcatg gcatccacaa caaataaaat ttcctgaggc tgaatggctt tttttatggc
      901 tgctatttcg ttcatcatcg tctcatcaac tgccaaacgc ccagcagtat ccacaatgac
      961 cacatcataa ccgtgaagtt tggcatattt tatggctgct tcggcaattg aaacaggatt
     1021 tttattgttt tcttcagaat acacaggaat tttaagctgc tcacccaaaa ctttcaattg
     1081 ctcgatagct gcaggtcgat atacgtcgcc ggcaaccaac aagggatttt ttcctcgttt
     1141 tgttttcaac agattggcaa gtttggccga aaaagtagtt ttacctgaac cttgcaaacc
     1201 ggccatcagg attacagccg gcgaaccttt cagattaatg tccacacttg agccgcccat
     1261 aagttgagcc aattcatcgt gaacaatttt tatcatcaac tggctgggct taatcgaagt
     1321 caatacattt tgacccaatg ctttttcctt taccgtatcg gtaaaggttt tggctgtctt
     1381 ataattgaca tcggcatcaa gcaaagcttt tctaacgtct ttcaatgttt cggccacatt
     1441 gatttcggtt atccgtcctt caccctttaa gattttaaaa gatctctcaa gtctttcaga
     1501 aagtgattca aacattttta attatcctcg tttatgaatt aattttattc cttaaatttt
     1561 tatttattgt cctttattcg catgctgtcc caaacaaaat acaaaatgag caatacatac
     1621 ataaagagtg ccaaccattc cgaaccgggc tgttgcagcc aactttcatt ggtgagttga
     1681 ataccgccaa aacgcaaggc agcgctgact acgcccatta atgcacctcc ggctataaat
     1741 cccgaagcaa gcaatgttcc tttttcctga cgcaaacggt ttaattcctt attgctgctt
     1801 cggcccgaaa cataccagct aacaagtcca ccaaccagca agggaacatt taattccagc
     1861 ggaataaaca ttccgagtgc aaaagccagc gcaggaacat tgaaaaaatt cagtaccaaa
     1921 gcaatcaggg ctcccatgcc atacaagagc cacggcgcac caactcccga catcaggggc
     1981 tcaattaccg ctgccatcgc attggcttga ggtgcaacca aagcattttc acctgtaaat
     2041 ccgtaagttt tgttcagaat cataataacg ccacccactg tagcagccga aacaagcgtt
     2101 cccaaaaatt tccagctttc ctgtttggcc ggcgttgttc caatccaata accaattttc
     2161 aaatcggtga taaagcctcc tgccatagac agggctgtgc aaaccacacc tcccataatc
     2221 aatgatgccg tcatacctgc cgttcctttt aagcccactg ctaccataat cacggaagaa
     2281 agaatcagcg tcatcaacgt catgcccgaa acgggattgg tacccacaat agcaatggca
     2341 ttggcagcta ccgtagtaaa cagaaaggca atgataccga tgaccagtat gccaaccaca
     2401 gcatgtgtca aattgccgac tacaccaaaa taaaaaaaca gaaaagtagc caacaatgct
     2461 ccgattattc caaagataat aattttcatt gaaatatcgc gctgggtacg tttgattttt
     2521 tgctgctcga gtgattttgc tccgcctttc agttcttttg cagccaaacc aaccgcatcc
     2581 ataataattt tccatgattt tgccacgccg ataatccctg ccatcgcaat tcctccaata
     2641 ccgatatgtc gagcaaaatc tctaaaaata atttcaggac tcatgacagc taaagttccc
     2701 tgaaacgccg gattcagcag gtgaattacc tgatcgttca aaagaggcaa tacaggaaca
     2761 actacaaacc aaatcagagc tgaacccgca caaataatgg cagcatattt taatccgacg
     2821 atatatccca atcccaatac ggcagctccg acattaactt tgaaaacgag tttggctttt
     2881 tcggcaattt gcgcgccaaa aggcaaaacc cgcgaagtaa aaacttcagc ccaagcgcca
     2941 aaagttgcga caataaaatc atacaagcca ccaattatgc cggctacaat caaaggtttg
     3001 gcctgatcgc cacccttttc gcccgaaaca agtacctgag tagtagctgt tgcttccgga
     3061 aaaggatact tgccgtgcat atccgaaaca aagtatttac gaaatggtat cagaaagagt
     3121 attcccaata cgccacccaa tagcgaactg agaaaaacct gcataaaatt caccgaaata
     3181 tcgggatatt tggcttgcag aatatataaa gcgggtaaag taaaaatagc gcctgccaca
     3241 ataacgcccg aacttgcccc aatggactgg ataatgacgt tttctcccaa agcatttttt
     3301 cgctttgcag ctcccgaaag tccaactgcg ataatggcta tgggaatagc tgcttcaaaa
     3361 acctgtccga cttttaaacc cagataggct gcagctgccg aaaaaagaac agccattagt
     3421 aatccggtcg aaacagagcg aaaatttact tccggaaaaa ccttttccgc agggagaatc
     3481 ggtttgtatt cttccccttc tttcaattcc cggtgtgcat tttccggtaa tcctgtaatt
     3541 ttgtcctctt ccattttatg atttcgttac gttcgttttt tcaactgaaa ttaattctca
     3601 attctgttat ttttactgct gatacaaaat acttgcagaa gaaggagcca caacaaaagc
     3661 ggttgttttc acctgagcaa tgccggtcaa attaatccgt ccgtcgtggc aaaccaaatt
     3721 ccattctcct tcgggaattt cgaccacaac agattttcga ttgccattgt aaataaccag
     3781 aatattgcgc caagtatctc ctttggcatt atcggtaagc gtaaacgcaa ctacattggg
     3841 aattccccaa tccaaaaaat gcaaatattg ttgaacattc tcttgtgtgc caagtctgaa
     3901 agcaggatga gctttgcgca gagcaattaa tcctttatag tattgataca aatcctgaaa
     3961 aacggtcttg ttgtcccaat tgatttggtt gatggaatcg ggcgactgat aagaattgtg
     4021 aacccccttt tttgttcgga acaattcttc tccggcataa ataaaaggaa tgccttgaga
     4081 agtaaaaaca atggtttgag ccagtttgtc gaatcgcaat atttcatcat tggttgctcc
     4141 taaaggtttg gaggaacgca acttgtcggc caagcacaaa tcgtcgtggc aggaaacata
     4201 gctgattacc tgggtaggat catttacata cggctcttta gaataaatca gtctggaata
     4261 atcaatttgg ggatgctgaa tggaaccgac tactccaaat tttacgcttt cctccaaact
     4321 gtccactccc gaaacaaagc cgggaatttc gggatgctgc cagctacctt tgagtgcatc
     4381 gcgtatgtcg tcgctaaaaa cggcaattct atccaattgc ttggcatttt ttttcagagc
     4441 tcttttttct tctggatata ccgatggacc agctgtccaa ccttctccat aaatacagat
     4501 agtaggatct actttgtcga gtgccaaccg tacggcattc atggtttcaa tatcgtgaac
     4561 gcccattaaa tcgaacctga aaccatcgat atggtattcg gtagcccaat aaacgactga
     4621 ttcgacgata aatttccgca tcatggcacg ttcggaagcc gtttcatttc cacatccgga
     4681 tgcatttgac catgttccgt cagggttcat gcgataaaaa tatcccggaa ccagcaaatt
     4741 gagatgcgaa ttttctccgg taaaagtatg gttgtaaacc acatccatta ccacgcgaat
     4801 tcctgattga tgcaaagctt gaaccatttg cttgaattct ttaattcgca cagccggatc
     4861 ataaggatcg gtggaataac ttccttcggg tacattgtaa tttaatggat cgtatcccca
     4921 attataatga ttttcattaa gttttgtctc atcaatggat gcaaaatcaa agctgggcaa
     4981 taaatgaaca tgagtaactc caagttcttt taaatggtct attccggtag cctgacccat
     5041 cgtatttttt gtcccatgtt cggtaaaagc aagaaattta cctttatttt tcattcccga
     5101 attgggggac atggaaaaat cacgaacatg tacctcatac agtatgatat cggcttcgtc
     5161 tttcaagggt ggtcgtacat ccatttccca gccggcagga ttagtttcta tcatatcgat
     5221 aatggcagct cttttgccgt taacaccggt tgcttttacc cacataccgg gagtttcatc
     5281 caaccatcga tcattaactt ttacctggaa agtgtaaaat ttacctttca aatcttcttt
     5341 tatccgcaat ttccatgttc cattttcact gcgcttcatg tttactactc gcgatgctcc
     5401 gtcatcaatg ccatcatcat aaagcaaaag cttgacttcc gcagccgtag gagcccatac
     5461 cctgaacgtg gaagcttcgg aagtataagt cagttcaagg tcagaaccat catagaccgg
     5521 ataatcgtcg taagaagcat attttggagt tttttggcaa gagcaaaacc atccggttac
     5581 caacatcata aatatcaaat gttttttcat tgttcgttat actttatttg aaatcaatta
     5641 tttgtactaa taattccgtt tcttctgagt aaagcatcga tagaaggttc ctgcccgcga
     5701 aaacgtttgt ataaaatcat gggatgctcg gtaccacctt tctttaagat attttccata
     5761 aaagattgag ctgtagcctg atcaaaaatg ccgttctttt caaaaacaga aaaagcatcg
     5821 gcatcgagta cttcagccca tttgtaactg taatatccgg cggcataccc gcccgaaaat
     5881 atgtgattga aagtaggact gatagccgta ttttccacgt aaggcaaaat ttgggttttc
     5941 gccacagcct gtttttcaaa gtctaccacg tcgccttcaa aaggtgaaga aagtgtatgc
     6001 caagccatat ccaaatatcc aaaaccaagt tgacgcaagc aggaatagcc gacattaaaa
     6061 ttttcggcat cctttatttt ttgaatcaac tcaaccggaa ttttttcacc ggtctggtag
     6121 tgaaaagcaa actgatccag aaattccttt tgggaagccc aattttccat gatttgtgag
     6181 ggcaattcca caaaatcgcg ataaacattt gttcccgaaa gcgatgggta agtacaatcg
     6241 gaaagcattc catgtagcga atgtccaaat tc
//
LOCUS       DAOSIB010000100         6206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_11424_length_6206_cov_11.016583, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000100 DAOSIB010000000
VERSION     DAOSIB010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11424_length_6206_cov_11.016583"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(421..777)
                     /locus_tag="PLD44_07885"
     CDS             complement(421..777)
                     /locus_tag="PLD44_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99286.1"
                     /translation="MYDLNRFFPEDNEMLTNFVEALFSANLQYSAMDLFRAGAESWKE
                     IDGAVKLSIQTLIKANLNPQMHFKFRFLTDIASGNIVQDWKMSKTGFVLTLLNIQGKN
                     PLLNKLKVEIIERYMS"
     gene            complement(795..1736)
                     /locus_tag="PLD44_07890"
     CDS             complement(795..1736)
                     /locus_tag="PLD44_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOF99287.1"
                     /translation="MEYKDYYKTLGVDKNATQEEIKKAYRKLAVKYHPDKNPGNKEAE
                     NQFKMINEAYEVLSDPEKRKKYDELGTNWNKFGEGNEYRGSNPFEGFGNRGGSQFYYQ
                     DNFNDLFGGKNTGFSDFFEAFFGKGATSGFGKSNRQTEFKGQDYETEINLTLEEAYHG
                     TSRIIQIDNEKIRIKIKPGVNDGQVLRIKGKGAKGSNPNYNGDLLIKVKVLPHPIFER
                     IGNDLKMIHQINLFDAILGGKTIIETLNGKIIIDIPEGTQNNKILRIKGKGMPVYGKS
                     GEYGDLLIKLQVIIPENLTPEQKNMFRRLKENLHSQK"
     gene            complement(1825..2259)
                     /locus_tag="PLD44_07895"
     CDS             complement(1825..2259)
                     /locus_tag="PLD44_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOF99288.1"
                     /translation="MALVKFSDQLPSLFDRLMNNDLFDWTFRNFSETNTTLPSVNIKE
                     TNDAYRLEMAAPGLKKEDFKIEVKDGVLSISSEKVGEELKEGEEFTHREYSYQSFVRS
                     FTLPDNVEEDKIEAKYENGILCVNIPKKEEAKAKPVKQIEIQ"
     gene            complement(2557..3672)
                     /gene="dprA"
                     /locus_tag="PLD44_07900"
     CDS             complement(2557..3672)
                     /gene="dprA"
                     /locus_tag="PLD44_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOF99289.1"
                     /translation="MDDSLKYKIALTMIHGIGNILAKNLIAYIGSVEGIFKEKRQNLA
                     KIPGIGEVLSKEIVSHSLIERAEKEIEFISKNQIKAIFFTEKDYPYRLKECPDSPILL
                     YCKGNTNFNEGKFVGIVGTRSATQTGKENCTKLVSDLAAMVPQLTIVSGLAYGIDIYS
                     HKAAVENHLPTIGVLGHGLDRIYPAAHRSTAIKMLAHGALLTEFISETNPDKPNFVKR
                     NRIIAGLCDALVVVESGIKGGALITAELANDYNRDVFAFPGRVDDIWSKGCNSLIKSN
                     KAFLMETAEDLLQIMNWEKLDSGSEIAPQVSMYFDLAEEEKEILFQLRQHADGLQVNE
                     LAIQMSKPFSKISSLLLQMEFKGLVKCLPGNIYRIIK"
     gene            complement(3665..4069)
                     /locus_tag="PLD44_07905"
     CDS             complement(3665..4069)
                     /locus_tag="PLD44_07905"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOF99290.1"
                     /translation="MFFVKYWKSLLVLFVILYLSFASPSTFKDIPTFQHADKVAHFLM
                     FALFTGVLINDFRHDKIVFTSKTEFLILSLAFPLFTGAIIEYLQGSVFQPRSAEWLDF
                     FSDTMGVVIGFLLMSALFHFLKKIKKIIRKDG"
     gene            4201..4992
                     /locus_tag="PLD44_07910"
     CDS             4201..4992
                     /locus_tag="PLD44_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOF99291.1"
                     /translation="MRKYIFLSCLTLLLVSCSSVLITGRKQLNFVPEREILSMSSQSY
                     KEFIASAPLSTDKTNTALVKKIGSNIASAVEIYLRNNGLASEIANLSWEFNLVKDTSK
                     NAFCMPGGKVVVFEGILPIAKNEDGLAVVMGHEIAHAIAKHSNERLSQQMLAQFGAEL
                     TNILLSNKSEATRIGINTLYGIGVEMGVMLPYSRKQEYEADRLGLIFLAMAGYNPQES
                     VAFWQRMAAASSGSNIEFLSTHPSDQNRIKKLQEFIPEAMKYYNK"
     gene            complement(5253..5777)
                     /locus_tag="PLD44_07915"
     CDS             complement(5253..5777)
                     /locus_tag="PLD44_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOF99292.1"
                     /translation="MAHIQSVRGIDPKIGKNCFLAETAVVIGDVTMGEGCSIWFNAVL
                     RGDVNSIKIGNHVNIQDGSVLHTLYQKSIIEIGDYVSIGHNVTIHGAKINDYALIGMG
                     AVVLDYAEIGEGAIVAAGALVLSNTVVPPYTLWAGVPAKFIKNVEPKQTNEINHRIAQ
                     NYSLYASWYQDKDK"
BASE COUNT         1898 a         1144 c          978 g         2186 t
ORIGIN      
        1 ttacctgaaa aaccgtttta aaacaggttt caaagttaac attttattgg taatgatgca
       61 ccaaaaagtt taattttcac ttgaatttac gattttttat ttaggggaga tatggaaatt
      121 aagacattgc catctgattt atgttcccaa cacttttttt cttggtaata atattcagtc
      181 aaaatgcatt gatagtatga acttttttaa taatctatag tgcaaattcc atccacactt
      241 aaaaaatata ttggcaatat ctgtcatgag ctaactttat ccgttttttc agaaatttcg
      301 aatttttcca caaatactta tcctcaattg atatgcgtta aatatgatgc agttttccaa
      361 tttttagata tcggtttttt attcgcaatg tcgaaaaaac atctgttttt ccttcttcat
      421 ttaactcata tagcgttcaa taatttctac ttttaacttg tttaaaagtg gatttttacc
      481 ctgaatattg agtaatgtca atacaaaacc cgttttgctc attttccagt cttgcactat
      541 gttgccactt gctatatcgg ttaaaaatcg aaatttaaaa tgcatctgcg gattcagatt
      601 ggcttttata agcgtttgta tggatagttt tactgcccca tcgatttcct tccatgattc
      661 agctcctgct cgaaacaaat ccattgcact atactgaaga tttgcagaaa aaagagcttc
      721 tacgaaattt gtcaacattt cattgtcttc ggggaaaaat ctgttcaaat catacataat
      781 ccatctatta attattattt ttgtgaatgc aaattttcct ttaatctacg aaacatattt
      841 ttttgttcgg gtgtaaggtt ctcgggaata atcacttgca atttaatcag caaatcgccg
      901 tattctccac tttttccata cacaggcata ccttttcctt tgatacgcag aattttattg
      961 ttctgtgtac cttcgggaat gtcaattatt atttttccat tcagtgtttc aataattgtt
     1021 tttccaccca gtattgcatc aaacaagtta atttgatgaa tcattttcaa gtcattgcct
     1081 atccgctcga aaattggatg gggtagtact ttgactttta tgagcaaatc gccattatag
     1141 tttggattgc ttccttttgc tccctttcct tttattctta atacttgtcc atcgttaact
     1201 cctggtttga tttttatacg tattttttcg ttatcgattt gaataattct gcttgtacca
     1261 tgatatgcct cttccagggt aagattgatt tctgtttcgt aatcctgccc tttgaattcg
     1321 gtttgacgat tacttttacc aaatcctgac gttgctcctt taccgaaaaa tgcttcgaaa
     1381 aaatccgaaa atccagtgtt tttaccacca aataaatcgt tgaaattatc ctgatagtaa
     1441 aattgacttc cgccccgatt gccaaaacct tcaaaaggat tacttcctct atattcgttc
     1501 ccttcaccaa atttgttcca gttggttccc aattcatcat attttttcct tttttccgga
     1561 tcggacagta cttcgtaagc ttcattaatc attttaaact gattttccgc ttccttgtta
     1621 ccgggatttt tatcaggatg gtattttacg gcaagtttcc ggtaagcttt tttaatttcc
     1681 tcctgagtgg catttttgtc tactccaagt gttttatagt aatctttata ttccatataa
     1741 aacaaatatt tcaagcaaca aattgttaaa ataaatttaa taaaggggaa cacaatagtt
     1801 ccccttcatt tgttaaagta aaatttattg aatttcgatt tgcttaaccg gcttagcttt
     1861 agcttcttcc tttttgggaa tatttacgca taatattcca ttctcgtact ttgcttcgat
     1921 tttgtcttct tctacattgt cgggcaaagt aaaagaacgt acaaatgatt gatagctgta
     1981 ttccctgtgg gtaaattctt ctccttcctt cagttcttca ccaacttttt ctgaagaaat
     2041 tgaaagcacc ccatctttaa cttcaatctt gaagtcttct ttttttagac ctggagctgc
     2101 catttccaac ctgtatgcat cgttggtttc tttaatgtta actgatggaa gagttgtgtt
     2161 tgtttcagaa aagttgcgaa aagtccagtc gaacaaatca ttattcatta aacgatcaaa
     2221 taatgatggc aattgatctg aaaattttac taatgccata gttaaaccct cctttcgttt
     2281 tataattcta aattttactc tacacctcaa gctattcaaa ttcaatacca gagcatttcc
     2341 aataatttaa tttgcccggt aaataactgt atttcagagt aattttttat cttatggttg
     2401 cagaagtaat cgaagaaggg aataaaatta ttgacagaat gacatatttt tacgctgtaa
     2461 tgccaaactg tcaatgtttg ttttttactt gactatatct gatagacaat tacttgtttc
     2521 accacaacca ctaatttctt ctctttttta tcagaattat ttgattattc gataaatatt
     2581 acccggtaaa cattttacca atcctttaaa ttccatttgc agcaacagcg aagaaatttt
     2641 actgaatggt ttggacattt ggatagccag ttcatttact tgaagtccat cggcatgttg
     2701 tctcaactga aacaaaattt ctttttcttc ttctgccaga tcgaaataca tggaaacctg
     2761 cggagctatt tccgaaccgg aatccagttt ttcccaattc attatttgaa gcaagtcttc
     2821 tgctgtttcc attaaaaatg ccttattcga ttttatcaga ctgttacatc ctttggacca
     2881 aatatcatcg acacggccgg gaaaagcaaa tacatccctg ttataatcgt ttgccaactc
     2941 ggccgttatc aatgccccgc ctttaatacc cgattctact accaccaaag catcgcagag
     3001 tccggctata atccggttac gctttacaaa atttggttta tctggatttg tttcactaat
     3061 aaattcggtc agcaaagctc catgagcaag cattttgata gccgtagatc tatgtgcagc
     3121 agggtatatc ctatccaatc cgtgacccag aacaccaatg gtaggcaaat gattttctac
     3181 agccgcttta tgagaataaa tgtcaatacc ataagccagt ccgctcacta tggtgagttg
     3241 aggtaccatt gcggccagat cggatacaag tttggtacaa ttttcttttc ctgtttgagt
     3301 ggcacttcgt gttccaacta ttccaacaaa ttttccttcg ttaaagttgg tgtttccttt
     3361 acagtacaac aaaattggag aatcgggaca ttccttcaat cgatagggat aatccttttc
     3421 agtgaagaaa atggctttga tttgattttt agaaataaat tcaatttctt tctctgctcg
     3481 ttcaataagg ctgtgtgata caatttcttt ggacaaaacc tctccaatac cgggaatttt
     3541 tgccaggttt tgtctttttt ccttaaaaat tccttccaca cttccaatgt atgcaatcag
     3601 atttttggca agaatgtttc ctatgccatg aatcatcgtt agggcaattt tatattttaa
     3661 ggaatcatcc atcttttcgg ataatctttt ttattttttt caaaaaatga aacaatgcag
     3721 acattaaaag aaatccgata actactccca ttgtatcaga aaaaaagtcg agccattctg
     3781 ctgagcgcgg ttgaaaaacc gaaccttgca ggtattcaat aatagctccg gtgaataatg
     3841 ggaatgccaa actcaatatt aaaaattcgg ttttggaagt gaaaacaatt ttatcgtgtc
     3901 gaaaatcatt tatcaaaacc cctgtaaata gggcaaacat aagaaaatga gcaaccttat
     3961 cggcgtgttg aaatgtagga atatccttaa aggtagaggg gctggcaaac gacagatata
     4021 aaattacaaa caataccaat aaagatttcc aatattttac gaaaaacatc ctcttttttt
     4081 ggaaatgttg catacaacta aaataatttt atcaagaaaa aataattaaa ttttgattat
     4141 ctttgcaaag ataaaacctg ttctcaaaat actaaaattt gtttttcaaa tatcattatt
     4201 atgagaaaat atattttttt aagttgtttg actcttttgc tggtttcctg ttcaagtgtt
     4261 ctcatcactg gcagaaagca attgaatttt gttccggaaa gagaaatttt atcaatgagt
     4321 agccaaagtt ataaagaatt tatagcttca gctcctttgt caaccgacaa aacaaatact
     4381 gcattagtaa aaaaaatagg cagcaacatt gcatctgctg ttgaaatata tttgagaaat
     4441 aacggtttgg cgtctgaaat tgcaaacctt tcttgggaat ttaatttggt aaaggatact
     4501 tcaaaaaatg ctttttgtat gccgggagga aaagtggtgg tttttgaagg cattttaccg
     4561 atagcaaaaa atgaagatgg gttggctgta gtaatgggac acgaaattgc acatgccata
     4621 gccaagcaca gcaacgaacg tctcagtcag caaatgctgg cacaattcgg agccgaatta
     4681 accaatattt tgttgtccaa taaatcggaa gctacacgga ttggcattaa taccctttat
     4741 ggaatcgggg tagaaatggg tgtgatgttg ccttattcaa gaaaacagga atatgaagct
     4801 gacaggcttg gtttgatttt tttggctatg gccggataca atcctcaaga atctgtggct
     4861 ttctggcaaa gaatggctgc tgcttcttct ggcagcaaca tagaattttt aagtacgcac
     4921 ccttccgatc aaaatcgaat aaaaaaactt caggaattta ttcctgaagc catgaaatat
     4981 tacaacaaat gattgtgata tcatgattca ccagtaaaat tatgtattta agcgcagata
     5041 cttggttaat ctgaaaagaa agaatttagt gtttgatgca cctttcgatg gatatctcaa
     5101 aaaatttttt tctttaaatt cttagatgtt tgctcatctt tcaaattcca aaaaaaatta
     5161 ttaactattt gaacattaat acggcatttt gatgagctta aaagttgcca agtgctgaaa
     5221 ttctttttgt agctttatgg caagagagat tattatttat ctttatcctg ataccagctg
     5281 gcatagagtg aataattttg ggcaatgcga tgattgattt cgttagtctg tttaggttcc
     5341 acatttttta taaacttagc aggaacgcca gcccacaagg tgtaaggagg aacaacagta
     5401 ttgcttaaaa ctaaagctcc ggcagcaact atggctccct ctcctatttc agcataatcc
     5461 aaaaccaccg ctcccattcc tattaatgca taatcgttga tttttgcccc atgaatggtt
     5521 acattatgtc caattgaaac ataatcccct atttcaataa tcgatttttg atagagcgtg
     5581 tgcaaaaccg atccatcttg gatattaaca tgatttccaa ttttaatgga attgacatcg
     5641 ccacgtaaaa ctgcattgaa ccaaatgcta caaccttctc ccattgtaac atcgccaatc
     5701 actactgccg tttctgccaa aaaacaattt tttccaattt ttgggtcaat ccccctgact
     5761 gattgaatat gtgccatgta tattttattt ttcaagtgaa atttcaaact gaattcaatt
     5821 tattaatgca actttagcgt tagacaaatt tcgtaataaa aattcatttg atgttaataa
     5881 ttttaatttt tttcttaatc tgttgttcaa cccagattac tcattagcca atttaaaaga
     5941 aaagtcaaac tcagaagagc tgttctatag tccggaaaac cagactcgcc tctttttaaa
     6001 gcgattttca gaagcagttt gttgttgatt ttttcaaaat atgcaccaat ggcaaaagtt
     6061 ttgtatctaa gtgtgtagat aaagtttttt gcgttttttc tgcaaaatgt acattctaaa
     6121 ccacgacata agattatggg ctatcattaa aaaaattaaa acggctttcc ttgcaaaaaa
     6181 gttttttaga ttaaaactgt caaagc
//
LOCUS       DAOSIB010000101         6044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_11815_length_6044_cov_12.897646, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000101 DAOSIB010000000
VERSION     DAOSIB010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11815_length_6044_cov_12.897646"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1467)
                     /gene="hisS"
                     /locus_tag="PLD44_07920"
     CDS             complement(103..1467)
                     /gene="hisS"
                     /locus_tag="PLD44_07920"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOF99293.1"
                     /translation="MNQKPSIPKGTRDFSPEEMTKRNYIFDTIKSVFRLYGFQQIETP
                     AMENLSTLLGKYGEEGDKLLFKVLNSGDFLSGISDAELLERNAARLSGKICEKGLRYD
                     LTVPFARFVVQNRDKISFPFKRYQIQPVWRADRPQKGRYREFYQCDADIIGSNSLFNE
                     LELIQIIEEVFCRLKINVCIKINNRKILSGIAEYIGEPEKLTDITTAIDKIDKIGIEN
                     VNEELLSKGINREALGKLQPLLQLKGSNQEKLAQLKALLFESAAGMKGITELETIFDL
                     CEKANVCVPIELDLTLARGLNYYTGAILEVKALDVEIGSISGGGRYDNLTGVFGWEGV
                     SGVGVSFGADRIYDVLYQLQLFPEILAEQTKILFVVFGEKELKFSLQWAQALRKENIN
                     TEIYAEPSKLKKQLGYADNKKIPFVAIVGENEMQEGKITFKNMATGEQQMISLEELIQ
                     RLKN"
     gene            complement(1564..2772)
                     /locus_tag="PLD44_07925"
     CDS             complement(1564..2772)
                     /locus_tag="PLD44_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013815623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOF99294.1"
                     /translation="MRYLYSIGIHIYRFLIFLASFFNPKARELYKGQKNALRYLKSNI
                     EENARYVWFHAASLGEFEQGRPIMEALKRQHPDLKILLTFYSPSGYNVRKDYRGADVV
                     AYLPPDTRRSARAFVKTVKPLKAIFIKYEFWPNFLLTLRAEGVPTYAVSAIFRPNQIF
                     FKWYGKWYLQLLTTFRRLFVQDQDSAVLLEKHGIHQVSVSGDTRFDRVFALFSEAKKL
                     PLVEAFVAGKPVIVAGSTWPEDEDLLVKYLKQHPKIKLILVPHEIHKNHLEQISKLLD
                     GKFIRYTEASENNIADYNCLVVDVVGILSSIYQYAKVAYVGGGFGVGIHNILEAAVWN
                     VPVVFGPNFQKFKEARDLIQLKGAFSINDYTSLEENLDKLLCDEKAGQMAGNYVRENI
                     GATELICNNL"
     gene            complement(2860..3129)
                     /gene="rpsO"
                     /locus_tag="PLD44_07930"
     CDS             complement(2860..3129)
                     /gene="rpsO"
                     /locus_tag="PLD44_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOF99295.1"
                     /translation="MYLTQEKKKEIFGQYGKSNTDTGSPEAQIALFSYRINHLTEHLK
                     SNKKDYSTERALIKLVGKRRRLLDYLKEKDINRYREIIKELGIRK"
     gene            complement(3235..3918)
                     /gene="mnmD"
                     /locus_tag="PLD44_07935"
     CDS             complement(3235..3918)
                     /gene="mnmD"
                     /locus_tag="PLD44_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HOF99296.1"
                     /translation="MDSELILTQDGSHTLFVPDIDECYHSTFGAIGESTLIFIQSGFN
                     CCEKDKINVLEVGFGTGLNAFLTLLEAENRNKKVFYTAFELFPLAVEKAEQLNYPEKV
                     SPEKRFFFEKLHTSIWGEEVPITPFFKLLKINADFIVSSLPSVYDLIYFDAFSPEKQP
                     EMWSQKIFRKIFDHCNENAILTTYCVKGVVRRALQNTGFVVEKLPGPPGKREFLRAVK
                     PSNADFTEK"
     gene            4052..5941
                     /locus_tag="PLD44_07940"
     CDS             4052..5941
                     /locus_tag="PLD44_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOF99297.1"
                     /translation="MANYLQVENLTKSFGDRLLFENISFTVTENQRVALIAKNGTGKT
                     TLLNILTGGEDYDSGNIVYRSDLRIGYLLQNPDFRKDLTVMDACLTSDSEIVRTIAQY
                     ELCLSSHNEQKMNEIVARMDALRAWDYELRVKQILTKLKITDFDQKIGELSGGQLKRV
                     ALAQVLITEPDLLLLDEPTNHLDLEMVEWLEDFLKRSSMSLLMVTHDRYFLDRICTDI
                     LEIDFQKLFHYKGNYTYYLEKRQERLDAFNAETDRTRQLYRTELEWMHRQPQARGTKA
                     KSRIDRFHEIEQRARQRFDDKVIKLNVKASYLGSKIFEAKNVSKSYGELKILENFNYV
                     FSRYEKLGITGNNGTGKTTFLKMLLGEVQPDSGTFDIGETVVFGYYSQEGLNFDEQMK
                     VIDVVQNIAEVIDLGNGKKMSASQFLQYFLFSPESQYDYVYKLSGGERRRLYLCTILM
                     KNPNFLILDEPTNDLDIVTLNVLEDYLQNFKGCLIVVSHDRYFMDKVVDHLLVFKGNG
                     ILKDFPGNYTDYREWNDLMEEQERENEKKLKQNLFGGLSQKNETNEKNRPKKLTYKEQ
                     KELETLEKEIPILENEKREIEIRLGSGEPNSEEMVQLSHRFSELMQLIDEKSFRWLEL
                     MENID"
BASE COUNT         1924 a         1169 c         1129 g         1822 t
ORIGIN      
        1 ggtgtgcaaa gttaatcatt tgttctgaaa aatcaaatat ttttttaatg ttgatatttt
       61 tggaagttta tttttccttc tgcgcgggag aaaattacat atttaatttt tcaaccgctg
      121 aatgagctct tccaacgaaa tcatttgttg ctcaccggta gccatatttt tgaatgtgat
      181 ttttccctct tgcatctcgt tttctccaac tattgccaca aaaggaattt ttttgttgtc
      241 ggcataaccg agctgttttt tcagtttgga gggttcggca taaatttcgg tattgatatt
      301 ttccttgcgt aatgcctgtg cccattgcaa gctgaacttc aattcctttt ctccaaaaac
      361 cacaaacaga attttggttt gttcggccaa aatttcaggg aaaagctgta actggtaaag
      421 tacatcataa atcctgtcgg caccaaagga aactcctaca cccgaaactc cttcccagcc
      481 gaaaacacct gtcaaattat cgtaacgacc tccacccgaa atgcttccaa tttccacatc
      541 gagagccttc acttccaaaa tagctcctgt ataataattt aaaccacgag ccaacgtcaa
      601 atcgagctct atcgggacac aaacatttgc tttttcacaa agatcgaaaa ttgtttccaa
      661 ctcggtaatt cctttcattc ctgcagccga ttcaaaaagt aaagctttca gctgagcaag
      721 tttttcctga ttagagcctt tcaattgcaa caaaggctgc aatttgccga gagcttcgcg
      781 attgattcct tttgaaagca attcttcatt cacattctcg ataccaatct tgtctatttt
      841 gtcgatagcc gtagttatat ccgtcagttt ttcaggttct ccaatatatt cggcaatacc
      901 cgaaagaatt tttctgttgt tgattttaat gcagacattg attttcaacc ggcagaaaac
      961 ttcttctatg atttgtatca gttccaattc attaaataga gaattgctac cgataatatc
     1021 tgcatcgcat tgataaaatt cacgataacg ccctttttgt ggacgatcgg cacgccaaac
     1081 gggttggatt tggtaccttt tgaacgggaa cgaaatttta tcccgatttt ggaccacaaa
     1141 acgggcaaaa ggaacagtca gatcatagcg aagccctttt tcgcaaattt tacccgacag
     1201 acgggcagca tttctttcca acagttctgc atcggaaatt cccgaaagaa aatctccaga
     1261 attgagaact ttgaacaaca atttatcgcc ttcttctcca tattttccta gtaatgtgga
     1321 taagttttcc atagcaggag tttctatttg ctgaaatcca tacaatcgaa aaacactttt
     1381 gatggtatca aaaatgtaat tgcgttttgt catttcttcg ggcgagaaat cgcgtgttcc
     1441 ttttgggatg gaaggtttct ggttcatcgt ttaaaaattt ttcttctgta aattgtgaaa
     1501 aataagttat aactgatgct gtgtgctttt cttcatcttt tctgttgaat gattccggaa
     1561 aaatcacaaa ttattacaaa tcaattctgt agcgccaata ttttctctaa cataattgcc
     1621 tgccatttgt cctgcctttt cgtcgcaaag caatttatcc aaattttctt ccagcgaggt
     1681 ataatcattg atggaaaaag cacctttcag ttgaatcaga tcacgggctt ccttgaattt
     1741 ttgaaaattt gggccaaata caaccggcac attccaaacc gctgcttcaa gaatattgtg
     1801 aatacctaca ccaaagcccc cacctacata agccactttt gcatactgat aaatggaaga
     1861 aagtattccc actacatcga ccaccaaaca gttataatca gcaatattat tctcggatgc
     1921 ttcggtatag cgtatgaatt ttccatccaa aagttttgag atttgttcca aatgattttt
     1981 atgaatttca tggggaacca atatcaattt gattttcgga tgttgtttaa gatatttcac
     2041 caacaaatct tcgtcttctg gccaagtact acctgccaca attaccggtt ttccagcaac
     2101 aaacgcttca actaacggaa gcttttttgc ttcggaaaaa agagcaaaaa ctctatcgaa
     2161 acgcgtatca ccactgacgc tgacttgatg aatgccgtgt ttttcaagta aaactgctga
     2221 atcttgatcc tgaacaaaca aacgtcggaa agtggtcaac aattgcaagt accattttcc
     2281 gtaccatttg aagaagatct gattggggcg aaaaattgcc gaaacagcat aagtgggaac
     2341 accttctgcc cgaagcgtca acaaaaaatt tggccaaaat tcatatttaa taaaaatggc
     2401 tttcagtggt ttcaccgttt ttacaaatgc acgtgccgaa cgacgcgtat caggtggcaa
     2461 gtatgctacc acatcggcac ctcgataatc ttttctgaca ttataacctg aaggagaata
     2521 aaaagtgagg agaattttta aatcgggatg ttgccgtttc aatgcttcca ttatcggtct
     2581 tccttgctca aattcgccca aagaggcagc atgaaaccac acataacggg cattttcttc
     2641 aatattgctt tttaaatatc ggagcgcatt tttctgccct ttgtaaagtt cacgagcttt
     2701 gggattgaaa aatgaagcaa gaaaaataag aaatctgtaa atatgtatac caatggaata
     2761 taaataacgc atcctttaat ttgcggaaag aggtttctaa attaaaaagg catttcaagt
     2821 agttttgaaa cgccttttaa aagttgacaa atgcctgaat tacttacgaa tgcccaactc
     2881 tttgataatt tcgcggtaac ggttgatgtc tttttccttc aggtagtcca acaagcgacg
     2941 acgtttacct accaatttaa tcaaagcgcg ttcagtacta taatcttttt tgtttgactt
     3001 caaatgctca gtcaaatggt tgatacggta agaaaacaat gctatctggg cttcaggcga
     3061 accagtatca gtattagact tcccgtattg accaaagatt tctttcttct tttcttgagt
     3121 taaatacata atgaaagttt tatgtttaat aatgaactgc aaaggtataa attttctttt
     3181 aattaacgaa cactcttttt tcaaattctt ttgaaaaaat tgagaattaa ttcgttactt
     3241 ttctgtaaag tcagcatttg aaggcttcac agcccgaaga aattcacgtt tccccggcgg
     3301 cccgggaagt ttttccacaa caaatcccgt gttttgtagt gctcttctga ccacaccttt
     3361 tacacaatag gtggtcaata tggcattttc gttgcagtgg tcaaaaattt tcctgaatat
     3421 tttctgcgac cacatttcag gctgtttctc cggcgaaaaa gcatcaaaat agataaggtc
     3481 gtaaactgac ggcaaagagg aaacgataaa atcagcgtta atcttcaaca acttaaaaaa
     3541 gggagtaatg ggtacttctt caccccaaat agaagtatgc agtttttcga aaaagaaacg
     3601 tttttccggt gaaacttttt cgggataatt cagttgttcg gctttctcaa ccgccaatgg
     3661 aaaaagttca aacgccgtat aaaaaacttt tttgtttcga ttttccgctt ccaacaatgt
     3721 cagaaaagca ttcagacccg tgccaaaacc aacttcaagc acattgattt tatccttctc
     3781 acaacagttg aaaccacttt gtataaaaat cagggtggac tcacctatcg caccgaaagt
     3841 agaatgataa cattcatcta tatcgggaac aaacaacgta tgtgaaccat cttgtgttaa
     3901 aatgagttcg ctatccattt attgaaaaca gaatttacaa tttattggca gtaaaattaa
     3961 taatttttca cgaggagtga aacacgcgaa atggcaaaca tttatcgacc caatgctcgg
     4021 aaagtgttat ctttgtatca aattattgaa tatggcgaac tatctgcaag tggaaaatct
     4081 caccaaatca tttggcgatc gactgctttt tgaaaatatt tcttttactg ttaccgagaa
     4141 tcagcgggtt gcactgatag ctaaaaatgg aacaggaaaa acaaccttat tgaatatcct
     4201 taccggtggc gaagattacg acagcggaaa tattgtttac cgaagcgact tgcgcatagg
     4261 atatttactt caaaatccgg attttaggaa agacttaacc gtgatggatg cctgcttaac
     4321 ttccgacagc gaaatagtga gaacgatagc acaatatgaa ctttgccttt cttcacacaa
     4381 tgagcagaag atgaatgaga ttgttgcgcg catggatgct ttgagagctt gggactacga
     4441 actgcgtgtc aaacaaattc ttacaaagtt gaaaataacc gattttgacc aaaaaatcgg
     4501 tgaactttcc ggtggacaac ttaaaagagt ggcactggct caagtattga ttacggagcc
     4561 ggacttgctt ttgctcgatg aaccgaccaa ccacttggat ttggaaatgg tagaatggct
     4621 cgaagatttt ttgaaacgat cttcaatgtc cctacttatg gttacccacg atcgttattt
     4681 tctcgaccga atttgcaccg acattttgga aatagatttt caaaaactct ttcattacaa
     4741 aggaaattat acttactatt tggaaaaacg acaggaaagg ctcgatgctt ttaatgccga
     4801 aaccgaccga acccgacaac tgtatcgaac agaattggaa tggatgcatc gccaacctca
     4861 agctcgcgga acaaaggcaa aatcgcgcat cgatcgtttt catgagattg aacagcgcgc
     4921 acgtcaacgt tttgatgaca aagtaataaa actcaatgtt aaagccagct atttgggttc
     4981 aaaaattttc gaagcaaaaa atgtttcaaa atcttatggc gaacttaaaa tactggagaa
     5041 tttcaattat gtgttttccc gttatgaaaa actgggaatt acaggaaaca acggaacggg
     5101 aaaaacaact tttttgaaaa tgctgctggg agaagttcag cccgacagcg gaactttcga
     5161 tatcggagaa actgtcgttt ttggttacta tagtcaggaa ggattgaatt ttgacgaaca
     5221 aatgaaagtc attgatgtgg ttcagaatat tgccgaagtg attgatttgg gtaacggtaa
     5281 aaaaatgtcg gcatctcagt tcttacagta cttcctcttt tctcccgaaa gtcagtacga
     5341 ttatgtttac aaacttagtg gaggtgaaag aaggcgcttg tatctctgta ccatcttgat
     5401 gaaaaatccc aattttttaa ttctggacga gcccacaaac gacctcgata ttgttacgct
     5461 gaatgtattg gaagattatt tgcagaattt taagggctgc ttgattgtgg taagccacga
     5521 ccgctatttt atggacaaag tggtagatca tttgctggta tttaaaggaa atggcattct
     5581 gaaggatttt ccgggaaatt ataccgatta ccgggaatgg aacgatttga tggaagagca
     5641 ggaaagagaa aatgagaaaa agttaaagca aaatttgttc ggtggcttgt ctcaaaaaaa
     5701 cgaaacaaat gaaaaaaatc ggcctaagaa attgacctac aaggaacaaa aagaattgga
     5761 gactttggaa aaagaaatcc cgattttaga aaatgagaaa agagaaatag aaatccggct
     5821 gggatcggga gaaccgaaca gtgaagaaat ggtacaatta tcccatcgat tttcggaact
     5881 aatgcaattg atcgatgaaa aatctttccg ctggctcgaa cttatggaaa atatcgactg
     5941 ataagattgc gagaaaaaga aaaattcaaa attgaattta atattgctaa agtttcgcac
     6001 ttcgttttaa tgatttgaaa gacagaaaat aaagaagtgc gatt
//
LOCUS       DAOSIB010000102         6012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_11903_length_6012_cov_8.309048, whole genome shotgun sequence.
ACCESSION   DAOSIB010000102 DAOSIB010000000
VERSION     DAOSIB010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11903_length_6012_cov_8.309048"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(659..1588)
                     /gene="nadA"
                     /locus_tag="PLD44_07945"
     CDS             complement(659..1588)
                     /gene="nadA"
                     /locus_tag="PLD44_07945"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOF99298.1"
                     /translation="MEKKELSEEINRLRKEKNAVILAHFYQEPDIQDIADHVGDSLAL
                     AQWAAQTDADIIVLCGVHFMGETAKILNPQKKVLIPDMNAGCSLADSCPADAFEEFVK
                     NHPDYTVISYVNTSAAVKALTDVVVTSTNARKIVESFPENEKIIFAPDRNLGNYINSV
                     TGRQMLLWNGACHVHEQFSVEKLVELKKEHPEALVLAHPECTKPLLMMADYIGSTQAL
                     LNFATASDKNEFLVATESGILHEMQKKNPNKHFIPVPPNDSTCACNECNFMRLNTLEK
                     VYQCLLNESPEILVEEKIRQKAVKPILRMLELS"
     gene            2467..3975
                     /locus_tag="PLD44_07950"
     CDS             2467..3975
                     /locus_tag="PLD44_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOF99299.1"
                     /translation="MKVFSTSVVRQLDEYTIKNEPIASIDLMERAAIGLCKKFLEIFS
                     SPLPVYVFAGPGNNGGDALALARMLLQKNYSVSVYLIHTGKLSADCEANKNRLIEKFT
                     GCLLDFSTDFYPVNFSENTVIIDGLFGSGLSGPLSGIYAEAVRWINNSGKTVVSIDIP
                     SGLAGEENKDLSQPIVKADYTFSLQFPKLSFFLVENESFVGKWEIIEIGIHPEAIRRE
                     PTDFYFLEKKDVIPFLPERKKFSHKGTYGHALIVAGSVGMAGAAVLSAQSAIRSGAGL
                     VSVHSAAANRIILQTKVPEALFENDKEENLISELKNLEKYTSLAIGPGIGTQEKTAEL
                     LLHILPNIIQPLVLDADALNILSKRKELLEKLSANTLLTPHPKEFERLFGADENSYDR
                     LLKMQLLAKNLHLVIILKGAYTRIALPDGSIYFNSTGNPGMATGGTGDVLSGILAGLL
                     AQNLPPEKAALLGVFLHGLAGDFALRHQSVESLTAGDVVENLGNAFQSLREQ"
     gene            4103..5389
                     /locus_tag="PLD44_07955"
     CDS             4103..5389
                     /locus_tag="PLD44_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOF99300.1"
                     /translation="MIKKILIFLFLSFSIVTFGQSLNLDTCQKRAKENYPLLKQYGLI
                     EKTADYNISNANKAYLPQVSLLAKATYQSDVTAIPETLGQVLTQLSGQPVSFPVLSKD
                     QYQAALEVDQLVWDGGMVNAQKKLIKASSEAEKQKLEVDLYALNDRINNLFFGIMLIK
                     EQLKVNQILQQELASNYQRMEAYLKNGLIQQADLDAVKVEQINAAQQETDLKSVLKSY
                     CQMLSVFTGMTVNEKTELEKPLLPVVNENIENKRPELSLFESQKNLLESQKKTILAGN
                     LPKIGLFIQGGYGRPGFDFFNNDFTSFYIGGVRLSWNIAGFYTQKNELKKIETSQKNV
                     DIMKETFLFNANLLTKQQMNEIDKLRVTLKNDEEIIRLRNNIKKATESKFQNGTITTT
                     DFLREINAENIARQTRTLHEIQLYMAVYQLKNTTNN"
     gene            5412..>6012
                     /locus_tag="PLD44_07960"
     CDS             5412..>6012
                     /locus_tag="PLD44_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99301.1"
                     /translation="MKALIFDKKNLVLMSLIVGLTSCHRLLENKYDASGTFEATEVVV
                     SAEANGKIMDFNLEEGQQLTENQEVGYIDSTQLYLTKLQLLASQKSVQSRVPDIKKQI
                     AVLQQQIETAKKEQKRIQNLFSANAASQKQLDDVNAQVSVLEKQLVAAKSNLESTSNS
                     ILNEKEALNIQIEQIQNQLEKCRITSPINGTVLVKYAEKG"
BASE COUNT         2082 a          982 c         1092 g         1856 t
ORIGIN      
        1 gaaacagaca atatttacat cgttagggtt tacaccaatt tattgaatgg ttcaaaaaac
       61 catctgagag cttttgtaaa aaatctttca tccagaaatg tggtttatca acctcagatt
      121 ttgtcacaaa gcgaatattc tgaaacaatt tactgaaatt aatataaata aaagctatat
      181 tcaactttag agggaaaaaa tttgttgatt cgttcaaatt tataacttct tcttaactac
      241 tcccgttttt atacaatctc tatactaact caagttagtt gtatggggct gtccaaaaaa
      301 tatgacagcc tcatttttat tggttattga acaaaaatat tcattagaga ttagaaatta
      361 tttaaattaa cgtgagctcg atataagaat atttatataa tcaattataa ataagctaat
      421 aaggttgatg aaaaggaagt attcacatgt tactaaagct ggttgggtca aataaggtct
      481 ttatattcag aaaacgaact tgttttttaa taaaatgttg ggtaattttt ccatcgaatt
      541 gatatttatc cgggtagaga cgtagcatgc tacgtctcta aattcagtat ttcaatccga
      601 taatttataa caataggttg ctttacggat aatcattaaa aattatacaa ttttttattc
      661 aacttaattc caacattcgc aaaataggtt tcacggcttt ttgcctgatt ttctcctcta
      721 ccaaaatctc aggactttca tttaaaagac attgatatac tttttcaagg gtatttaaac
      781 gcataaaatt acattcgttg catgcacagg tgctatcgtt gggaggaaca ggaataaaat
      841 gtttattggg atttttcttt tgcatttcgt gtaaaatacc cgattctgta gccaccaaaa
      901 actcattttt atccgaagcg gtagcaaaat tcaataaagc ctgcgtactg ccaatataat
      961 cggccatcat taaaagtggt tttgtacatt caggatgagc caaaaccaat gcttcgggat
     1021 gttccttttt taattccacg agtttttcta ctgaaaattg ttcgtgtaca tggcaagctc
     1081 cattccacaa aagcatttgt cggccggtta cgctgttgat ataatttccc aaatttctgt
     1141 cgggagcaaa aataattttc tcattttcag gaaaagattc cactatcttc ctggcgttgg
     1201 ttgaagttac taccacatca gttagagctt ttactgctgc agaagtattg acataagaaa
     1261 taaccgtata atcgggatga ttttttacaa attcttcaaa ggcgtctgcc ggacaactgt
     1321 cagcaagtga acaaccggca ttcatatcgg gtatcagcac ttttttttgc ggattaagta
     1381 tttttgctgt ttctcccata aaatgcacac cacaaagaac aattatatcg gcatccgttt
     1441 gagcagccca ttgagccaaa gccaaactgt cgccaacatg gtcggcaata tcctgaatat
     1501 ctggttcctg ataaaaatgt gccagaatca cagcattttt ttcttttctt agtctgttta
     1561 tttcttctga aagctctttt ttttccatag ctttgttgtt atcaaatgta tatcatatta
     1621 atattttata gatttaaata aattatatga taataatgat agggtgttaa tttggttgat
     1681 aatttattga ataaagactt gtttaattaa ttattaaatt ttatagtata tttgtgaacc
     1741 aaatgaaata ataaaagtat ttgaaaaata gaattttaca aatattatca acaagttgtt
     1801 tataagtgca aagttagtaa gtttttattt tttcaggtga ataaatatca tgaatttggt
     1861 tgaaaaaata cgcaaaagta tttcagttta aattttcaaa atccaaaaca aatgatatgg
     1921 aaaattgttt ttggtctgtc gataaaaaga aataaatgat tataaaatag ttttcaacag
     1981 cttttcgggg ctgaatgttg ataagtataa actctataaa aaaaggctta gtacatgaca
     2041 aaaaaattgt agaaacattt atattctata ttttcagata tttaataacg gggctttgcc
     2101 ccaaactcca cttacttttt tgtcttgaaa caaaaaagta agcaaaaaaa ttcaagactg
     2161 tgtccacttc actcgaaaaa cttacgctcg aaaagctgaa atcgcccaaa ctcattccga
     2221 cgaaatacgt cggaactcaa acaaggacga tttttaaacg cttttctcac ttgtttttcg
     2281 gctcaccgga caaggtcaaa cccaagagct tggcttatat gtcctgtttt aaagatatat
     2341 aaaacaaatg tctcaatttt tgtaatgtac taaaaaagga ataaaaaaac caattcagta
     2401 ataaagataa ttcaactata tgaatgtaaa tatattacca aagaaaataa tttaatatct
     2461 tgagcaatga aggttttttc aacttcagta gttcgtcaat tggacgaata taccataaaa
     2521 aatgagccaa tcgcatccat cgatttaatg gaaagagcag ctattgggct ttgtaaaaaa
     2581 tttttggaaa tattttcttc tcccctaccc gtctacgttt ttgccggacc gggaaacaac
     2641 ggtggcgatg cgctggcttt ggcaagaatg ctgcttcaaa aaaattatag tgttagtgta
     2701 tatttgattc ataccggaaa actttctgca gattgcgaag ccaataaaaa cagactgata
     2761 gaaaaattta caggttgttt gcttgatttt tctaccgact tttatcctgt aaatttttct
     2821 gaaaataccg tcataataga tggtcttttt ggttcgggtt tgtcgggacc attgagcgga
     2881 atttatgcgg aagcggttcg gtggataaat aattctggaa aaaccgttgt gtccatagat
     2941 attccttccg gattggcagg tgaagaaaac aaagatttat cgcaacccat agtgaaagcc
     3001 gattatacat tttcgttgca atttccaaag ttgtcgtttt tcttagtaga aaacgaatct
     3061 tttgtaggaa aatgggagat aatagaaatt ggtattcatc ctgaagccat tagacgagaa
     3121 ccaaccgatt tttatttttt agaaaaaaaa gatgtaattc cgtttttacc ggaaagaaaa
     3181 aagttttccc acaaaggtac ttacggacat gcgttgatag tggctggaag tgtgggaatg
     3241 gcaggagctg ccgttttatc ggctcaatcc gccataagaa gtggagcagg attggtaagc
     3301 gtgcattcgg ctgcagcaaa cagaattatt cttcagacaa aagtcccgga agccttgttt
     3361 gaaaacgata aggaagaaaa tttgatttct gaattaaaaa atttggaaaa atatacttct
     3421 ttagccatag gtcccggtat tggcactcaa gaaaaaaccg ctgaactttt actgcacatt
     3481 cttcctaaca tcatacaacc gcttgttttg gatgcggacg cattgaacat tttgagcaaa
     3541 agaaaagaac tattggaaaa actttcggct aatacccttc ttacgcccca tcccaaagaa
     3601 tttgaaaggc tttttggtgc ggatgaaaat tcttatgacc gtttattgaa aatgcagcta
     3661 ctggcaaaaa atttgcatct ggtcattatc ctgaaaggag catatacacg aattgccctt
     3721 ccggacggca gcatctattt taacagcacc ggaaatcccg gaatggctac aggaggaaca
     3781 ggtgacgttt tgtcaggaat attggctggt ttactcgctc aaaaccttcc tcccgaaaag
     3841 gcagcacttt taggcgtttt tttgcatgga ctggccggtg attttgcttt acgacatcaa
     3901 agtgtggaat cattaactgc aggcgatgta gtagaaaatt tgggaaatgc ttttcaatcg
     3961 ttgagagagc aataaagttt tgatataatt ttttgtattt caaaaaaatc aacttgaaaa
     4021 aatttagatt tttaaatatt accacaacga ataatttaca gaaaaaaact ttttacaaac
     4081 cttcataaaa agtgaaagta ttatgataaa gaaaatattg atttttctgt ttttatcttt
     4141 ttcgattgtt acgtttgggc aatcattgaa tcttgatact tgccagaaaa gagctaaaga
     4201 aaattatccc ttgttgaaac aatatggatt gatagaaaag acggccgatt acaatatttc
     4261 caatgccaat aaagcctatt taccacaagt cagcttgctg gcaaaagcta cttatcagtc
     4321 agatgtaacc gctattcccg aaacacttgg tcaagtttta acacagttga gcgggcaacc
     4381 ggttagtttt cccgtactga gcaaagatca atatcaggcc gcattggaag ttgatcagct
     4441 ggtttgggat ggcggaatgg taaatgctca gaaaaaattg ataaaagctt cttcagaggc
     4501 tgaaaaacag aagttggaag tggatttgta tgcgttaaat gaccgcatca ataatttgtt
     4561 tttcgggatt atgctcataa aagaacaatt gaaggtgaat cagattcttc agcaagaatt
     4621 ggcaagcaat tatcagcgaa tggaggctta tttgaaaaat ggattaatac aacaagccga
     4681 cctggatgct gtgaaagtgg aacaaatcaa tgctgcacaa caggaaaccg atttaaaaag
     4741 tgttctgaaa tcgtattgtc aaatgttgtc ggtgtttacc ggcatgactg tgaatgaaaa
     4801 aacagagctt gaaaaaccac ttttacctgt tgtaaatgag aatattgaaa ataaacgtcc
     4861 ggaactttcg ctttttgaat cacagaaaaa tttattggaa agtcaaaaga aaaccatttt
     4921 agctggaaac ttgcctaaaa taggattatt tattcaaggt ggctacgggc gaccgggatt
     4981 tgattttttc aacaatgatt ttacttcctt ttatatcgga ggtgtgagac tttcatggaa
     5041 tatagccgga ttttatacac agaaaaatga attgaaaaaa atcgaaacca gtcagaaaaa
     5101 tgtggatatt atgaaagaaa cttttctttt caatgccaac ctgcttacca agcaacaaat
     5161 gaacgaaata gacaagcttc gtgtgacatt gaaaaacgat gaagaaatta tacgtttgcg
     5221 gaacaatata aaaaaagcta cggaatcaaa atttcaaaat ggaacaatta ctacaaccga
     5281 ttttttacgt gaaataaatg cagaaaatat cgcccgtcag accagaacac ttcacgaaat
     5341 tcaactatat atggctgttt atcaattgaa aaatacaaca aataattaaa atttgaaacc
     5401 atctgaaaaa tatgaaagct ctaatttttg acaaaaagaa tttggtgtta atgagtttga
     5461 ttgttggatt aacatcgtgt catagacttt tggaaaacaa atacgatgct tccggaactt
     5521 ttgaagctac ggaagtggtc gtatcagctg aagctaacgg taaaataatg gatttcaacc
     5581 tcgaagaagg tcaacagttg accgaaaatc aggaggttgg ctatatagac agtacgcaac
     5641 tttatctgac aaaattacaa ttactggctt cgcaaaaatc tgttcagagt cgagttccgg
     5701 atattaaaaa gcaaattgcc gttcttcagc aacaaataga aacagcgaag aaagaacaaa
     5761 aacggattca gaatctattc agcgccaatg ctgccagcca aaagcaactc gacgatgtca
     5821 atgcgcaagt ttcggtgctc gaaaaacagc ttgtggctgc aaaatcgaat cttgaatcga
     5881 ccagtaatag tattttgaat gaaaaagaag ctttgaatat tcagatagag caaatacaaa
     5941 atcaattgga aaaatgcaga attaccagtc cgattaatgg aaccgtgcta gtgaaatatg
     6001 ccgaaaaagg gg
//
LOCUS       DAOSIB010000103         5930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_12110_length_5930_cov_11.017702, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000103 DAOSIB010000000
VERSION     DAOSIB010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12110_length_5930_cov_11.017702"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..2757)
                     /locus_tag="PLD44_07965"
     CDS             complement(244..2757)
                     /locus_tag="PLD44_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOF99302.1"
                     /translation="MTVNYSEQLHNILLYSLQEAERLGNNYIGPEHLLLGIIRNGKGK
                     AIDILNHTQVDLSKIKQSIESQIRTDNEPTGEEILSLKSTERIKKIMELETRSLSSET
                     ADSEHLLLAILKEKNNLAVEVLNEENLTYEIAKSYLEHSKDIQMSSNFQDDDDEDDEP
                     RQHQRNVSGSGKQSDTPALDTFGTDITKAALENRLDPVVGRQVEIERLAQILSRRKKN
                     NPVLIGDPGVGKSAIVEGLALRIVQHKVSRVLFDKRIVALDMASIVAGTKYRGQFEER
                     IKAILNELAKNPNIILFIDEIHTIVGAGGAPGSLDAANMLKPALARGEIQCIGATTLD
                     EYRQSIEKDGALERRFQKIIVEPTSYEETLQILRNIQDKYEYHHNVRYTPAAIEACVR
                     LTERYITDRCFPDKAIDALDEAGSRVHIGSVSVPKEIEELEKRIEELRQQKMKVLSEQ
                     KYELAGPIRDQEKQCQYQLEDAIRRWEQESQQHPEIVDEEQVAEVVAMMSGIPVQRIA
                     QAEGLKLRQMKDVLQSKIIGQEEAVNKIVKAIQRNRIGLKDPNKPIGSFIFIGPTGVG
                     KTYLAKVLAEFMFDSPDALIRVDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLTEKVRR
                     KPYSIVLLDEIEKAHPDVFNLLLQVMDEGRLTDSLGRRIDFKNTILIMTSNVGTRQLK
                     DFGKGVGFNTIETPEMDAEFSQGVIQKALSKTFAPEFLNRIDEVIVFNPLSKDAIKSI
                     IDLELKGLFGRITSMGYRLELTPEAKDFIAEKGYDAQFGARPLKRAIQRYLEDELADI
                     VLSGDLETGDTILMDLDAENGKMKAKIVKPAPVLNQNNN"
     gene            3170..4366
                     /locus_tag="PLD44_07970"
                     /pseudo
     CDS             3170..4366
                     /locus_tag="PLD44_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924840.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4993..5604
                     /locus_tag="PLD44_07975"
     CDS             4993..5604
                     /locus_tag="PLD44_07975"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOF99303.1"
                     /translation="MRNLWRQLRTEGIEDERVLKAMANVPRELFVGNSCMEEAYENTP
                     LPIGHGQTISQPFIVAYMTECLHVEPDDFVLEIGTGSGYQAAVLAQLAKKVYSIEIIK
                     ELGISAKERLKKLHYDNVEIIIGDGYYGWLEHAPYDKIIVTAAANEIPPPLVEQLKNQ
                     GRMVIPVGFPYTTQHLMLITKSGGKIASTTLLPVSFVPFTGKH"
BASE COUNT         1622 a         1119 c         1171 g         2018 t
ORIGIN      
        1 agagggattc gaacccccgg aacctttcag ttcaacggtt ttcaagaccg ccgcaatcga
       61 ccactctgcc atctctccaa catttctcaa aatgcggtgc aaatatactt ctttttcttt
      121 tatcctacaa caatagttgc ggttttaaag agatatttgg cacgattttc attcaaaaca
      181 gacaaaaaaa atacctgcca ttgcatcgaa tagcaggtac atttttgatt tttgcttgat
      241 attttagttg ttgttttggt tcagtaccgg agcaggttta acaatttttg ctttcatttt
      301 gccattttcg gcatccaaat ccattaaaat tgtatcgccc gtttccaaat ctcccgacag
      361 cacaatgtct gccagttcat cttcaaggta tcgttgaata gcacgtttca gagggcgtgc
      421 accaaattga gcatcgtaac ctttttcggc aatgaaatct ttggcttcgg gtgtcaattc
      481 gagtcgataa cccatagaag taattcgtcc aaaaagtcct tttaattcaa ggtcgataat
      541 agatttaatg gcatccttgc tcaacggatt aaatacgatt acttcgtcta tacggttcag
      601 aaattcggga gcaaatgttt tgctcagcgc tttttgaatt acaccttgtg aaaattctgc
      661 atccatttca ggtgtttcaa tggtattgaa tcctacgcct ttaccaaaat ccttcagttg
      721 tcgcgtaccg acattggaag tcataatcag aatggtattt ttaaaatcta ttctgcgacc
      781 caagctgtca gtcaatcttc cttcatccat tacctgaagg agaaggttga aaacgtcggg
      841 atgcgctttt tcaatttcat ccagcaagac tattgaataa ggtttgcgtc gaactttctc
      901 ggtcagctga ccaccttctt cgtaacccac atatcccgga ggtgctccga taagtcgcga
      961 tacgctgaac ttttccatgt attcgctcat atcgacgcga atcagtgcat cgggactgtc
     1021 gaacataaat tcggcgagca ctttggccag ataagttttt cctacgccgg tagggccgat
     1081 aaatataaaa gaaccaatcg gtttatttgg atccttcaat ccaatacgat ttctttgaat
     1141 ggcttttaca attttgttca cagcttcttc ctgcccgata attttgctct gaagtacatc
     1201 cttcatttgt cggagtttca gtccttctgc ttgagcaata cgctgcacgg gaatgcccga
     1261 catcatggca accacttcgg caacttgttc ttcgtctaca atttcgggat gctgttgcga
     1321 ttcttgttcc catcgtctga tcgcatcttc cagctggtat tggcattgtt tttcctgatc
     1381 gcgtattgga ccggccaatt catatttctg ttccgaaaga actttcattt tttgctgacg
     1441 aagttcttcg atacgttttt ctaattcttc aatttctttc ggtacggaaa ctgagcctat
     1501 atgaacgcgc gaaccggctt cgtccaatgc atcaatagct ttgtcgggga agcatcgatc
     1561 tgttatgtaa cgttcggtaa gtcttacgca agcttcaatg gctgccggcg tataacgcac
     1621 gttgtgatgg tattcgtatt tatcctgaat gttgcgtaaa atctgcaatg tttcttcgta
     1681 agaagtcggt tctacaataa ttttctggaa tcgacgttcc aacgctccat ctttttcgat
     1741 gctttggcgg tattcgtcca aagtggtggc accgatacat tgaatttcac cacgtgccaa
     1801 tgcgggttta agcatgttgg cagcatccag tgaaccgggt gcgccaccgg caccaacaat
     1861 ggtgtgaatt tcatcaataa aaagaatgat gttcggattt tttgccaatt cgttgagaat
     1921 agctttaata cgttcttcaa actgtccgcg gtattttgtt cctgcaacaa tggaagccat
     1981 atccaatgca acaattcgtt tgtcgaagag tacacgcgaa actttatgtt gaacgatgcg
     2041 caaagccaaa ccttctacta tagccgattt accaactccc ggatcaccta tcaatacggg
     2101 attgtttttc tttctacggc taagaatctg agccaatctt tcaatttcga cttgtctgcc
     2161 tactacggga tccaacctgt tttcgagagc agctttggta atatctgttc cgaatgtgtc
     2221 caaagccgga gtatcactct gtttgccact tcccgaaaca tttcgctgat gttggcgagg
     2281 ttcatcatct tcgtcatcat catcctgaaa attggaactc atttgaatat ccttagaatg
     2341 ttcaagatac gatttggcaa tttcataggt cagattttct tcatttaaaa cttctacagc
     2401 caaattgttc ttttctttca gaatggccaa aagcaaatgt tcagaatcgg ctgtttcgga
     2461 tgaaagagaa cgagtttcaa gctccatgat ttttttaatg cgttcggtgc ttttcaaact
     2521 taaaatttct tccccggtag gttcgttatc tgtacgaatc tgactttcga tagactgttt
     2581 tatttttgac aagtccactt gcgtgtggtt gagaatgtcg atggctttcc ctttgccgtt
     2641 tctgattatc cccagcagca aatgttcagg ccctatataa ttgtttccca acctttcagc
     2701 ttcctgaagg ctgtaaagaa gtatgttgtg taactgttct gaatagttga cggtcatatt
     2761 tagatttctc cttttttcta caaaaataat taatttaatg tgatgttgga cactcaaagt
     2821 ttgactttag aaattttaat tttttataag aaaacgaatg gattaaaatt ttttcatgtc
     2881 aacattagta aaactttaat cttgaaatcg tttaatgtgc ttattattat caaacttttc
     2941 actccaaaat tttattttat ctatcaaaaa taataccgat ttgtttttat cgtgtcttct
     3001 gaaattttgt cagtataaag tgaaccgttg tcaggctgca aaaaaatatg aataggaatg
     3061 cctgcatgaa attgttttca aaaatttttt cagtagtttt gcatttcttt atttttaaat
     3121 accaagaaca taaattcaat aaaaaaacaa ccattaaaga aaattgttta tgtcgcagat
     3181 aaaatcagtt tcattacgtg aatcaccagt ggcacgttgg acggctttgg gaatagtttc
     3241 ctttacgatg ctttgcggat atttcattgc cgatgccatg agtcctctta aaccattgct
     3301 ggagagtcag ttgctatgga gtagcattga ttattcgctg tttaccagtg cttatggatg
     3361 gttcaatgta tttctgttga tgcttatttt tggtggtatc attttggaca aaatgggtgt
     3421 tcgttttacc ggattggcct ctacagctgt catggtgatt ggggcagcca tcaagtattt
     3481 tgccatatcg ccttttttcc ccgatgatgc cgggactatt ctgggcatca aggctcaggt
     3541 aatgatagcg gcattgggat atgccacatt tgccgtcgga gtagaaattg ccggcattac
     3601 ggtttctcgt gtcattgtca aatggtttaa aggaaaagag atggcgctgg ctatgggact
     3661 tcaaatggct acagctcgac ttggaacttt gctggccgtc gctattccgc tccctgttgc
     3721 caaacatttt aatgatgttt cggttcctat cctgtttggc gttttactgc tctgtattgg
     3781 acttatcgct tatattgttt acgttcagat ggacaaaaag ctggaagctt cagaagcaaa
     3841 tgatgaaaag gaagaggaag aaccgtttaa agtttcggaa gttttcacta ttattaaaaa
     3901 taaaggtttt tggcttattg ccattttgtg tgttctgttt tattccgcag tttttccgtg
     3961 gataaaattt gccaccgata tggttatcaa caagtatcag gttaatcccg acttggcagg
     4021 gcttatcccg agcgttcttc ctttgggaac attgtttctt actcctttat ttggaaatat
     4081 ttacgaccga aaaggcaaag gtgcttccat tatgattctt ggcgctttat tgcttatatt
     4141 tgttcatgca attttttcta ttccatttct caataattgg gtcttggcta ttggcgtaac
     4201 gatagttttg ggaattgcct tatcgcttgt accttcggcc atgtggccat cagttccgaa
     4261 aattattccg gaaaggcagt tgggaacagc ttatgcattg attttctgga tacaaaattg
     4321 gggattaatg ggtgttccct tgttgatcgg attggtgctg aacagtgcaa atcctacggt
     4381 ttacaaagcg aaagaaatgg tgaagtccgg agtaatagag acttatgaac agtttcaacc
     4441 ttcattggga ctgaataaaa ttgaatttga gaaagccgtt gataaaacta ctacggcttt
     4501 aatcaatgaa gtgttgttga aaagtgatta tgaaggcgtg caggataccg taggttttgc
     4561 agcctttaaa aatgaatgga tacataaaaa tattaccgct ttgccattgg aaaaactaaa
     4621 ttcttttcaa gacaaaaatc agaaacttcg agcggtagaa aatacttgct tagagattgg
     4681 ttattcggaa atcgtaaaaa ataaaattgc tttgaggtac gactatacac gtccaagtct
     4741 ggtatttatg atttttggag ttctggcctt gctgtttgct ttcttgttga aaaaggaaga
     4801 taaagcaaaa aactatggtt tggagttacc caatattcaa aaggaataat tttttcagga
     4861 ttttgtattt atagaaaagg cggtttgaaa attttcaggc tgcttttttc ttttatgagc
     4921 aaacaataat ttaaagaaat gtgtttttat aattgataat aagcaaatta tttttggagt
     4981 ttttattttg acatgagaaa tctttggcga caactcagaa cggaaggaat tgaagacgaa
     5041 cgtgtgctga aagcaatggc gaacgtacca cgcgaattgt ttgtaggtaa ttcttgtatg
     5101 gaagaagctt atgaaaatac gccgttaccc attggacacg ggcaaactat ttctcagcct
     5161 tttattgtag cttacatgac cgaatgtttg catgttgaac cggatgattt tgttcttgaa
     5221 atcggaacgg ggtccggtta tcaagcggca gtacttgctc aattggcaaa aaaagtttat
     5281 tcgatagaaa ttataaaaga attgggaatt tctgccaaag aacggttaaa aaaattacat
     5341 tacgacaatg tagaaataat aattggagat ggttattacg gttggctgga acatgctcct
     5401 tacgataaaa ttattgttac tgccgctgca aacgaaattc cgccaccact tgtagaacaa
     5461 cttaaaaatc agggacgaat ggtaattccg gttggttttc cctatactac acaacatttg
     5521 atgctgatta caaaaagtgg cggaaaaatt gcttccacca ctctgttgcc tgtgagtttt
     5581 gttcccttca ccggaaaaca ttgaagagaa gagtaatgtt ttacgttgcg ccaaatctac
     5641 agtccattgc ttgtttctat tttcgtaatt tcgctgccgt tcaatttaag caaccaattg
     5701 gcctctatca ttaactgatg ccatattttt ttcaatactt agttgcatca ttgaccggat
     5761 tttgtatctt tgttctaagg ttcaatgttt tttatgaaaa aattgtttga attgtttgaa
     5821 ttgttgaatt taggaaatat ttttctaaaa tctgcattaa ttcaccagtt attattttgt
     5881 tatttttaaa caaattctgt gatttataat ttttgcttta ttttatgaaa
//
LOCUS       DAOSIB010000104         5278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_13982_length_5278_cov_12.001340, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000104 DAOSIB010000000
VERSION     DAOSIB010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13982_length_5278_cov_12.001340"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            390..462
                     /locus_tag="PLD44_07980"
     tRNA            390..462
                     /locus_tag="PLD44_07980"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:423..425,aa:Phe,seq:gaa)
     gene            complement(671..2035)
                     /gene="miaB"
                     /locus_tag="PLD44_07985"
     CDS             complement(671..2035)
                     /gene="miaB"
                     /locus_tag="PLD44_07985"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOF99304.1"
                     /translation="MGLKENTHTSVTENPHKKLYIETYGCQMNVADSEVVASIMQMDG
                     FVLTEDFSEADAVLINTCSIRDNAEQRVFERLKYFHSLKRKNKQLILGVIGCMAERMK
                     EELIEKQGVDLVAGPDAYLDLPNLMGAVEKGEKAINIQLSRTETYQEVIPARLGGQIS
                     GFISITRGCDNFCSYCIVPYTRGRERSRDVESILNELKDLQQKNFKEVTLLGQNVNSY
                     RVEQDGKIIGFPELLEIVAQAAPEMRFRFTTSHPKDMSDRTLETIARYPNLCRFIHLP
                     VQSGSNKILRLMNRKYTREWYLERIAAIRRILPDAAIGTDIFCGFHDETEEDHKQTLS
                     LMREVAFDMAFTFKYSERPGTYAARHLPDNVSEEVKLRRLNEIIALQNELSLESNLRD
                     VGKTFQVLVEGYSKRSKEQFFGRTSQNKVVIFPRQGRRIGETVKVKINSASSATLFGE
                     VEHL"
     gene            2551..3585
                     /locus_tag="PLD44_07990"
     CDS             2551..3585
                     /locus_tag="PLD44_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99305.1"
                     /translation="MVKWIRGFFLLGLFIPFSLQSQVLKGELGLNYFFDNTEFSSSTL
                     TIDQTMKGVHLIPGVAVEWDKNNSLHGGTDLLNISGSQKTIEKIDLVAYYQFQSRKTL
                     FRAGSFPRQNILSNYSDFFFKDSIRYFRPNLQGLFWQVSNPTSFFNLWLDWTGHQTAT
                     QRESFFIGASANKRQHLFFADFQSYMFHYANTAPRNPEYHVCDNLLAHLSAGVDLTSL
                     TQLDTLMFAVGILEGFERERGMDNMTFTPSGLVVRNEIEYGKVGVSNTLYWGDARMKL
                     YEKHGWNLYWSNPFFRSGSYFESKWYWNMLKSRQVNGKLAMEFHVSEGKVFFEQLFTL
                     SASVDKLLKK"
     gene            3640..5178
                     /locus_tag="PLD44_07995"
     CDS             3640..5178
                     /locus_tag="PLD44_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOF99306.1"
                     /translation="MLVKTFGAAVQGINATIITIEVNVNQGIRFFLVGLPDNAVKESH
                     ERIASALDYIGYKMPHKQVVINMAPADIRKEGSAYDLPLAIGILAADGKINPDVLQNY
                     VIMGELSLDGSLQPIKGVLPISIKAREEKFKGFILPKQNAREAAVVNNLEVYGVENLK
                     EVIDFLNGKNQLEPTVVNTREEFFNSLNQSEVDFAEVKGQENVKRALEVAAAGGHNII
                     MIGPPGAGKSMLAKRIPTILPPLTLHEALETTKIHSVAGNIDSNTSLIAERPFRSPHH
                     TISDVALVGGGTFPQPGEISLAHNGVLFLDELPEFKRTVLEVLRQPLEDRKICISRAK
                     FTIEYPASFMLVAAMNPCPCGYYNHPEKECVCAPGVVQKYLNRISGPLLDRIDIHIEI
                     VPVPFEKLSEKRDAESSAQIRERVIKARQIQELRFRDIDGVYCNAQMTTKLQRMFAVP
                     DKEGLELLKNAMQRLNLSARAYDRILKVSRTIADLDDSEKILPYHIAEAINYRNLDRE
                     GWAG"
BASE COUNT         1620 a         1064 c         1001 g         1593 t
ORIGIN      
        1 cctgtgaatt ctttttacaa catttagaaa tcagaaatta aatgtagaat gtagtttttg
       61 aacaaatgat tgtcatgcaa aatcggtaga tgaatatgat agtcaggatg tagcaaattt
      121 tgggacaaag aaaaaagaag aaactatatg atggacaact tgtatttttc gacaaataaa
      181 atacttcgag gcttgctcta gatgattcat tacattgatt cttaatttta tttacataaa
      241 cacttgatac aggatacttt aaggataatc gttattttga aaaaagtcaa ggttaaaccg
      301 caattttttc aaaaatatga aattttttag ctgagaaact ttgagaataa atagaaaagt
      361 actatctttg cattctcaaa aataaaaatg gtaccatagc tcagttggta gagcaaagga
      421 ctgaaaatcc ttgtgtcccc ggttcgattc ctggtggtac cacaaaaaag cactttaaat
      481 tgtcagattt aagtgctttt tttggttcgc tatttaaaat taggtgtttt aaaaaaattg
      541 gtcaatttaa ttagaaagaa tttgatatcg aatattctta tttcatcctc taaatgactt
      601 catcatagat tcgaccgata aacgccaaac acaaataacc tgaaattaaa aaagtctttt
      661 tcttcagaaa ttacagatgc tctacttcgc caaataaagt tgccgacgaa gccgagttaa
      721 ttttcacttt caccgtttcg cctattcgcc ttccctgacg cggaaaaatc accactttat
      781 tttgtgaagt tcgtccgaaa aattgctctt tggaacgttt ggaatagcct tccaccagta
      841 cttgaaaagt ttttcctacg tcccgcaggt tgctttcgag cgaaagttca ttctgcaaag
      901 cgataatctc attcaaacga cgcaatttca cttcttcgct cacgttatcg ggcaaatgac
      961 gagcagcata agttcccggg cgttcggaat atttaaaagt aaaagccata tcgaaggcta
     1021 cttcccgcat caacgaaagt gtttgcttgt gatcttcttc cgtttcatca tgaaacccgc
     1081 aaaaaatatc tgttccaata gcagcatccg gtaaaattct tcggatagcg gcaatgcgtt
     1141 ccaaatacca ttccctggta tattttcggt tcatcagccg taaaattttg ttgcttccgg
     1201 attgcacggg gagatgaata aaccggcata aatttgggta tcgagcaatg gtttcaagtg
     1261 tgcggtcgct catgtctttg ggatgagaag tggtaaaacg aaaacgcatt tcgggtgcag
     1321 cctgagctac aatttccaac aattcaggaa atccgattat ttttccatcc tgttccacac
     1381 ggtaggaatt aacgttttgt cccaaaagtg taacttcttt aaaatttttc tgctgcaaat
     1441 ctttcaattc gttaagaata ctttccacat cacgacttct ctctctacct cgcgtataag
     1501 gcacaatgca ataggaacaa aaattatcgc atccacgcgt aatggaaata aaacctgaga
     1561 tttggccccc cagtcgggca ggaatcactt cctgataggt ttccgtacgc gaaagttgga
     1621 tattaatggc tttctcacct ttttcgacgg ctcccatcaa gttgggtaaa tcgagataag
     1681 catcaggccc tgccaccaag tcaacgccct gtttttctat caactcttct ttcatccgct
     1741 cggccataca gccaatcaca cccaaaatca actgtttgtt ttttcgtttc aacgaatgaa
     1801 aatatttaag tcgttcgaac actcgttgtt cagcattgtc gcgaatggag caggtattga
     1861 tcaatacggc atcggcttct gaaaaatctt ctgtcagtac aaagccatcc atttgcatga
     1921 tggaagccac cacttcactg tcagccacat tcatttgaca accgtaagtt tcgatataaa
     1981 gttttttatg tggattttcc gtaacggaag tatgagtatt ttcttttaat cccatgattc
     2041 aagctttaaa aaaatgctta caaaggtaca acattaaagg aaattttatg gttaaaaatg
     2101 ttttagcttt atttaactac atgttaaata gttcattatt taattgattt cttttaattt
     2161 acatcaacag ataaaaaaag aatcgagaaa aaaagaattt ttttttgcaa tcgtttgcaa
     2221 aattcaaaat taagtttcat ctttgcaacc gtaaacaaac agataaattt ttcatagtta
     2281 aggtttaggt taaaaggtac aatgggtggc tgggaagtta cccattgtta ttttgagccc
     2341 cccatcaatc aaggcttatc taaataaaaa tttttgcgtt tatattttga accttacttc
     2401 ttcccattat ctattttcct ataatttttt gtttttagtc ataatattcg ttattcatac
     2461 tcaattttta agaaaaaaca gctaaacccc accaaaatat gaagataatc tgtatttttg
     2521 cttaaaatta attttaagtt gaaaaatttt atggttaaat ggattcgggg tttctttctg
     2581 cttggcctat tcatcccttt ttcccttcaa agtcaagtgt taaaaggcga attgggatta
     2641 aactattttt ttgacaacac cgaattttct tcttctaccc ttaccatcga tcagaccatg
     2701 aaaggcgttc atttaattcc aggagtggct gtggaatggg acaaaaataa ttccttacac
     2761 ggaggcactg atttattgaa catttccggt tctcaaaaaa ccatagaaaa aattgatttg
     2821 gtggcttatt atcaattcca atcacgaaaa actcttttcc gtgccggttc ttttccgcgt
     2881 caaaacatac tttccaacta ttccgatttc ttttttaaag attccatacg atatttccgt
     2941 cccaatttac agggactttt ctggcaagtt agcaatccga cttcattttt taacctttgg
     3001 ctcgattgga caggacatca aacagccacc caacgggaat ctttttttat tggagcgtct
     3061 gccaacaaac gacaacatct gttttttgcc gattttcaat cttatatgtt tcattatgcc
     3121 aacacggcac cacgtaatcc tgaatatcat gtttgcgaca atttgctggc ccatctttcc
     3181 gcaggagtcg atctgacctc tcttacacaa ttggacacgc ttatgtttgc ggtcggaatt
     3241 ttagagggtt ttgagcgcga acgtgggatg gacaatatga cttttacgcc ttccggttta
     3301 gttgttcgaa atgaaattga atatggcaaa gttggcgttt ccaatacgct ttattggggc
     3361 gatgcgagaa tgaaattgta cgaaaaacat ggctggaatc tttactggag caatccattt
     3421 tttcgatccg gcagttactt tgaaagcaaa tggtattgga acatgctgaa aagcaggcaa
     3481 gtgaacggaa aattggctat ggaattccat gtatcggaag gaaaagtatt ttttgaacag
     3541 ttgttcaccc tttcggcttc ggtggataag ctgttaaaaa aataagctat actgcagaaa
     3601 taaaattaat tatctgaatt acacctaaaa atattcctca tgctggtaaa aacttttgga
     3661 gctgctgttc aaggcattaa tgccaccatt attaccattg aagttaatgt aaatcaggga
     3721 attcgtttct ttctggtagg acttcccgac aatgcggtaa aggaaagtca cgaacgcatt
     3781 gcttccgcat tggattatat aggctataaa atgcctcaca aacaagtggt tatcaacatg
     3841 gccccggccg atattcgtaa agaaggttca gcttacgact taccgctggc tatcggcatt
     3901 ttggctgccg atgggaaaat taatcccgat gtgcttcaaa attacgtcat tatgggcgaa
     3961 ttgtcgcttg atggaagttt gcaaccgata aaaggtgttc tgcctatttc catcaaggca
     4021 cgcgaagaaa aattcaaggg atttatcctg cccaagcaaa atgctcgcga agctgccgta
     4081 gtaaataatt tggaagttta cggagttgaa aacttaaagg aagtaatcga ttttctgaat
     4141 ggcaaaaatc aactcgaacc aacagtagta aatacgagag aagaattttt caacagcctg
     4201 aaccagtccg aagtagattt tgccgaagtg aaagggcagg aaaatgttaa aagagcgctc
     4261 gaagtggctg ctgctggcgg acacaatata atcatgatag gtccgccggg cgcaggaaaa
     4321 tcgatgctgg ccaaaagaat ccccactatt cttccacctc tgactcttca tgaagcattg
     4381 gaaacaacca aaattcattc cgttgccggc aatatcgaca gcaacacttc gctcattgct
     4441 gaacgcccgt tccgaagtcc acatcatacc atttcggatg tagcactggt gggcggaggt
     4501 acatttcctc aacccggaga aatttcgctt gcccacaacg gtgttctttt tctggacgaa
     4561 ttgcctgaat ttaaaagaac agtgctcgaa gtgttgcgcc aaccattgga agacaggaaa
     4621 atatgtattt cgagagccaa atttaccata gaatatcccg ccagctttat gctggtagca
     4681 gccatgaatc cctgtccttg cggctattac aaccatccgg aaaaagaatg tgtttgcgct
     4741 ccgggtgtgg tacaaaaata tctgaatcgc atttcagggc ctctgctcga cagaatagac
     4801 attcatatcg aaattgttcc tgtgccattt gaaaaacttt ccgaaaaaag agatgccgaa
     4861 agtagcgccc aaatcagaga acgagtgatt aaggctcgtc aaattcagga actgcggttc
     4921 agggatattg atggcgttta ttgcaatgcc caaatgacca caaaattgca acgcatgttt
     4981 gccgttcccg ataaggaagg gctggaatta ttgaaaaatg ccatgcaacg actcaatcta
     5041 tcggcacgcg cttacgacag aattctaaaa gtttcgcgaa caattgccga cttggacgat
     5101 tcggagaaaa ttctaccgta tcacattgcc gaagccatta attacagaaa cctcgaccgg
     5161 gaaggctggg caggataaaa actgcgcaaa caaaaaaacc acctttgcgg tggttttgct
     5221 cttcctcttg gacttgaacc aaggaccctc tgattaacag tcagatgctc taaccgac
//
LOCUS       DAOSIB010000105         5270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_14003_length_5270_cov_11.517354, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000105 DAOSIB010000000
VERSION     DAOSIB010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14003_length_5270_cov_11.517354"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLD44_08000"
     CDS             <1..409
                     /locus_tag="PLD44_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99307.1"
                     /translation="FSKGEKFYSDSLFSSSGKGINNSNGDICLFFNILTYDGTDYRFL
                     ENLPEVLSFLGEIDSKGDAVFWAYLNGYYFIFGYPASGIKQTSNGFLIYAYNMVKMCT
                     PVQIDKFWLKIDKTGNIEVLGEKLFSRIENGCI"
     gene            complement(462..536)
                     /locus_tag="PLD44_08005"
     tRNA            complement(462..536)
                     /locus_tag="PLD44_08005"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(499..501),aa:Asp,seq:gtc)
     gene            840..1706
                     /gene="purU"
                     /locus_tag="PLD44_08010"
     CDS             840..1706
                     /gene="purU"
                     /locus_tag="PLD44_08010"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOF99308.1"
                     /translation="MKGNDNTAILLIHCPDRPGILAAVTEFINRHQGNILYLDQYVNK
                     EEKVFFMRVEWDLKDFQIPKEKIEEYFNTLIAIRFEMIFRLYFADEVPRMAIFVSKLS
                     HCLYDLLARYAAGEWKVEIPLIISNHKDLEPVAKRFGIDFYTFSINEKNKAEQEALEL
                     QLLKEKKVTFCVLARYMQILSPEFIQHYPNKIINIHHSFLPAFAGSKPYHAAYERGVK
                     IIGATGHYVTEDLDAGPIISQDVTHISHKDNVEELIRKGRDLEKIVLSAAVEKHINRK
                     ILTYKNRTIVFQ"
     gene            1725..3488
                     /locus_tag="PLD44_08015"
     CDS             1725..3488
                     /locus_tag="PLD44_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOF99309.1"
                     /translation="MEISRLYDILDRYLEKFPNQDAALACKREGKWRKFSIQEYVDLT
                     NQISYGMLKLGIEPGDRIGIVSNSRPEWNMLDFAAMQIGAISIPIYATISQDDYRYIL
                     NHSEMKMIFIEGKELRLKVMPILPEIKSLKYVYTFVDQGTGLPFLEQLIQLGRENPAP
                     EKLQQLKAMQKPGDMATIVYTSGTTGNPKGVMLSHSNIVNQILSLHQIPAEWVKVALS
                     FLPLCHAYERMLVYLYQYLGISVYYAENLGTIGENIKEVNPNIMATVPRVLEKMYDKL
                     YMAGKKLPTISKFIYYRAVHFANKYQLEGMSWWSKKLHKFFDKMVYSKWREAIGGNFD
                     IVVSGGSALQPQMASFFSAIGMPVFEGYGLTETSPVIAVSQRVKNGRKFGTVGLPIPG
                     VEVKLGDQDEILCRGHNVMLGYYKDPELTASVIDPDGWFHTGDIGKFTPEGQLIITGR
                     IKSLFKTSNGKYVNPEAIETKFAESPFIENMIVVGENKKFPAALISPSFEYLKSWCEN
                     HKIPCGSPEEMIENEHVIKRFRDEVKHYNNFLGEFEQIKRFQLVAEEWTPQNGFLSPT
                     LKLKRNVIEEYYAEKIEKLFS"
     gene            3534..4292
                     /gene="truA"
                     /locus_tag="PLD44_08020"
     CDS             3534..4292
                     /gene="truA"
                     /locus_tag="PLD44_08020"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOF99310.1"
                     /translation="MRRRYFIYISYKGTAYHGWQCQPNGISVQEVLIKTLSAILRTDI
                     NLVGAGRTDAGVHAKCMVAHFDYENEISDFSRFLLHLNSFLPPDIAVYKIVEVKPTAH
                     ARFDAVFRRYEYHVVFHKDVFKRELATRLFAELDFETMNKASEMLRNYQDFGSFCKLH
                     TDVKTNICHIHFAHWNQVEDEWVFTIQADRFLRNMVRAIVGTLFEVGRGKMTIEEFCS
                     VIKAGNRSKAGMSAPAEGLYLVDIQYPEEIFKLS"
     gene            4300..5055
                     /gene="hisF"
                     /locus_tag="PLD44_08025"
     CDS             4300..5055
                     /gene="hisF"
                     /locus_tag="PLD44_08025"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOF99311.1"
                     /translation="MLAKRIIPCLDVKDGKTVKGINFENIKEVGDAVELGDWYAKRGA
                     DELVFLDITATNEKRKTLSELVTRIARKINIPFTVGGGISSVEDVSLLLSCGADKISI
                     NTAAVKNPRLISELARQFGSQCVVLAIDTKYIDGEWYVFLNGGRIPTDIKTLDWAKKA
                     VALGAGEILLTSMNHDGTKDGFALDITCSVSESVNVPVIASGGAGKMEHFVDVFQKGK
                     ADAALAASIFHYREIDIPDLKKFLHENHIPVRW"
BASE COUNT         1591 a          915 c         1147 g         1617 t
ORIGIN      
        1 tttttctaag ggagaaaaat tttattcgga ttcgttattt tcttcttcag gcaagggcat
       61 aaacaattca aacggagata tttgtttgtt tttcaacatt ttgacttatg atggaacaga
      121 ttatcgtttt ttggaaaatc ttccggaagt gttaagtttt ctgggcgaaa tagacagcaa
      181 aggagatgca gttttttggg catatttgaa tggttactat tttatttttg gatatccagc
      241 ttctggtatt aaacaaactt cgaatggttt tttaatttac gcgtataaca tggtaaaaat
      301 gtgtaccccg gtgcagattg ataaattttg gctgaagatt gataaaacgg gcaatattga
      361 agtgctgggc gaaaaattgt ttagcagaat agaaaacggc tgtatttaaa acatatattg
      421 atcatatcaa acaaaaaagc aacccttaaa ggttgctttt gcggtgcgta cgggactcga
      481 acccgtgacc ccatgcgtga caggcatgta ttctaaccag actgaactaa cgcaccaaaa
      541 atttattttt cacaaatttc ttttaaattc ttttctgctt tcctgttttt atttcggact
      601 caacaaccgt tgttgttcgg tgaaatttgc ggtgcaaagt tacgctaaat tttaatatct
      661 gcaatatctt tgtgaagttt tttgaatttt cttccattat tttctttgtt tggaagttac
      721 cgcacagaac gagaatacac atttcaatat ttcgcaggtc aatgtttgta cagaagtctc
      781 gaaaacattt ttcctcaaaa agtattatat ttgcagacta aaaaaattga agaatcttta
      841 tgaaggggaa tgataatacc gccatcttat tgatacattg tcccgacagg cccggtattt
      901 tagctgctgt aaccgaattt atcaaccgtc atcagggaaa tatactttat cttgatcagt
      961 acgtcaacaa agaagaaaag gtctttttta tgcgtgtgga atgggatttg aaagattttc
     1021 agattccaaa ggaaaagatc gaagaatatt tcaacacact tattgctatt cgatttgaaa
     1081 tgattttcag attgtatttt gccgatgaag tgcctcgcat ggctattttt gtttccaagt
     1141 tgtcccattg tctttacgat ttgctggctc gttatgccgc tggagaatgg aaagtggaaa
     1201 ttcctttgat aatcagcaac cacaaagatt tggagccggt tgccaaacgt tttggaatag
     1261 atttttatac tttttccatc aatgaaaaga ataaggcaga acaggaagca ctggaattgc
     1321 aactgttgaa ggagaaaaaa gtaacttttt gtgtacttgc ccgttatatg cagattttat
     1381 cgcccgaatt tattcaacac tatcccaata aaattattaa cattcatcat tcttttttgc
     1441 cggcttttgc aggttccaaa ccttatcatg ctgcttatga aagaggagtg aaaattattg
     1501 gtgcaacagg ccattatgtt accgaagact tggatgcggg gcctattatt tcgcaggatg
     1561 taacccatat ttcgcacaag gacaatgtgg aagagctgat tcgcaagggt cgcgatttgg
     1621 aaaaaatcgt gctgtcggct gctgtagaaa agcacattaa ccgtaaaatt cttacttata
     1681 aaaacagaac cattgttttt caataatttt taaaaatcaa tatcatggaa atcagtcgtt
     1741 tatatgatat attggatcgt tatttggaaa aatttccgaa tcaggatgct gcccttgcct
     1801 gcaaacgcga aggaaaatgg cgcaaattca gcattcagga atatgtggat ttgaccaatc
     1861 aaatcagtta tggaatgctg aaactcggta tcgagccggg cgaccgcata ggcattgtga
     1921 gcaatagccg tcccgagtgg aatatgctcg attttgcagc catgcaaatt ggagccatat
     1981 cgattcctat ttatgctacc attagtcagg atgattatcg atatattttg aatcattccg
     2041 aaatgaaaat gatttttatc gaaggcaagg aattgcgact gaaggtaatg cctattttgc
     2101 ccgaaataaa gtcgttgaaa tacgtttata cttttgtaga tcaaggtacg ggtcttcctt
     2161 ttttggaaca gcttattcag ttgggaagag aaaatcctgc tcccgaaaaa ttgcaacagc
     2221 ttaaagctat gcagaaaccg ggcgatatgg caaccattgt ttatacttca ggaaccactg
     2281 gaaatcccaa aggtgttatg ctttctcaca gcaatattgt caatcagatt ttaagtttgc
     2341 accagattcc ggccgaatgg gttaaagttg ctttgagttt tctcccgctt tgtcatgctt
     2401 acgagcgaat gctggtttat ttgtatcagt atctcggaat ttcggtgtat tatgccgaaa
     2461 atttgggaac catcggcgaa aatattaagg aagtaaatcc caacatcatg gccactgtgc
     2521 cgcgcgtgct ggaaaaaatg tacgataaac tctacatggc agggaaaaag ttaccgacta
     2581 tcagtaaatt tatttattac agagccgtac attttgccaa taaatatcag ttggaaggca
     2641 tgagctggtg gagcaaaaaa ttgcataaat ttttcgacaa aatggtttat tccaaatgga
     2701 gagaagccat tggaggaaat ttcgatattg ttgtctccgg cggttcggct ctgcaaccac
     2761 aaatggcttc gtttttttcg gctatcggca tgccggtgtt tgaaggttat ggcttaacgg
     2821 agacttctcc tgtgattgct gtcagtcagc gggtaaaaaa cggcagaaaa tttgggacgg
     2881 tgggactgcc aattcccgga gttgaagtta aacttggcga tcaggacgag attctttgtc
     2941 gggggcacaa tgtgatgctg ggatattaca aagatcccga gttgacggcc agcgttattg
     3001 accctgatgg ttggtttcat accggcgaca taggaaaatt tacacctgaa ggccaactga
     3061 ttattaccgg acgaataaaa agcctcttta aaacttcaaa cggtaaatat gtgaatcccg
     3121 aagccattga aacaaaattt gcagaatctc cgtttataga aaacatgatt gttgtgggtg
     3181 aaaacaaaaa attccctgct gcactcattt cgccaagttt cgagtattta aaatcgtggt
     3241 gcgaaaacca taaaattcct tgtggcagtc cggaagaaat gattgaaaac gagcacgtaa
     3301 taaaacgttt tcgggatgaa gtgaagcatt acaacaattt tcttggcgaa tttgaacaaa
     3361 taaaacgttt tcagttggtt gccgaagaat ggactccaca aaacggattc ctttcgccaa
     3421 ctttaaaatt gaaaagaaac gttattgagg aatactatgc cgaaaaaatt gaaaaattgt
     3481 tttcgtgata attgcaagtt tttcttattt tctgaactat tcagtttttt ggcgtgagaa
     3541 ggcggtattt tatttatatt tcttacaaag gaacggccta tcacggttgg cagtgccagc
     3601 ccaacggtat ttcggttcag gaagtattga taaaaacgct gagtgccatt ttgcgaacgg
     3661 atattaattt ggttggtgcc ggtagaaccg atgccggtgt tcatgccaaa tgcatggtgg
     3721 cacattttga ttacgaaaat gagatcagcg atttttcaag gttcttattg catctcaaca
     3781 gttttttgcc tccggatata gcggtttata aaattgttga agtaaaacct accgctcatg
     3841 cccgtttcga tgccgttttc aggcggtatg aatatcatgt ggtttttcat aaagatgttt
     3901 tcaaacgtga gctggccacc cgcctgtttg ccgaattgga ttttgaaacg atgaacaagg
     3961 catcagaaat gctacgcaac tatcaggatt ttgggagttt ttgcaagttg catacggatg
     4021 ttaaaaccaa tatttgccat attcattttg cacattggaa tcaagtggaa gatgaatggg
     4081 tgtttaccat tcaggccgac cgttttctgc gaaatatggt acgagccatt gtgggaacgc
     4141 tttttgaggt gggaagaggc aaaatgacca ttgaagaatt ttgttccgta attaaagccg
     4201 gaaatcgcag caaagcaggc atgtcggctc cggcagaagg attatatttg gtggatattc
     4261 aatatcctga agaaattttt aaactgtcat aaaaaattta tgttggcgaa acgcattatt
     4321 ccctgtctgg atgtaaaaga tgggaaaacc gtaaaaggaa ttaatttcga gaacatcaag
     4381 gaagttggtg atgctgtaga gttgggcgat tggtacgcta aaaggggagc ggatgagttg
     4441 gtctttttgg atattacggc cacgaacgaa aaacgcaaaa cgctttcgga attggtaaca
     4501 cgcattgccc gtaaaatcaa tattcccttt accgtcggtg gcggcatttc cagcgtggaa
     4561 gatgtgtcgc tgctgctttc atgcggagca gataagattt ctatcaacac ggcagccgta
     4621 aaaaatcccc ggttaatttc cgaattggca cgacagtttg gaagtcaatg tgtagtattg
     4681 gccatcgaca ccaaatatat cgacggcgaa tggtatgttt ttttgaatgg tggaagaatt
     4741 ccaactgata ttaaaaccct cgactgggca aaaaaagctg tggcgttggg agccggagaa
     4801 attttgctga cttccatgaa tcacgatggt acgaaagatg gttttgcttt ggatattact
     4861 tgttcagtat ccgaatcggt aaatgttccg gtaatagcca gtggtggggc agggaagatg
     4921 gaacattttg tcgatgtttt tcaaaaagga aaagccgatg ctgcgctggc tgccagtatt
     4981 tttcattatc gggaaattga tattcccgac ttgaaaaaat ttcttcacga aaatcacatt
     5041 ccggtaagat ggtaagaaat ttataatggg aaattttggg tcggaataag atcgtcctca
     5101 cgctgccgca caaattcttt cgtgtaacaa aaattgtaaa tcaacatcat tccttgtaaa
     5161 catatccatc cgttctgcaa atgcaaaact tacaaaatat acttcttcag gattgtgaaa
     5221 tttataattt ctactcaagt ttcaaagata gttgattttt taaaaataac
//
LOCUS       DAOSIB010000106         5234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_14122_length_5234_cov_12.346399, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000106 DAOSIB010000000
VERSION     DAOSIB010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14122_length_5234_cov_12.346399"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1381
                     /locus_tag="PLD44_08030"
     CDS             191..1381
                     /locus_tag="PLD44_08030"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF99312.1"
                     /translation="MNRKKNKRIFGYPRNIFFTGLTSFLTDTSLKMVYSVMPMFLLSI
                     GASKTSLSLIEGVAESTASLIKTITGFWSDKIGKNKPFLLIGYGLSAVIIPLYSLVVS
                     PIQVLILRFVERVGKGIRTAPRDSIVAGSVENGETGKSFGLQKAMDNSGAILGPLIAF
                     ILLSAFKDNYRLIFLLAGIPSILGILVIIFGITEAKKNKESLVKKFRFGDFSKKFYFF
                     LAIVFIFTLGNSTDALLLVKANEIGIKVAFIPLVYLLTNVVSVLLSIPVGSLSDRVGK
                     EKILIVGYIIYALVYFGFGASSHIVVIASLFALYGVYSAATDGIQKAFVSDIVDENKK
                     GTGLGLYNALLGITLLPASLIAGILYDKVNSHVPFYFGAVTALIAAVLMFWFQRSFKK
                     QVVE"
     gene            1528..2760
                     /locus_tag="PLD44_08035"
     CDS             1528..2760
                     /locus_tag="PLD44_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOF99313.1"
                     /translation="MVLNYIWIGFFLIAFVIACIQLLVFGNVQVFSDMLNSTFDSAKS
                     GFEISLGLTGVLSLWLGIMKVGERGGVIRLFSKAVAPFFEKIFPDLPKNHPAYGSMFM
                     NIAANMLGLDNAATPLGLKAMKEMQETNPVKDTASNPMIMFLVLNTSGLTLIPVTIMV
                     YRAQMGATNPSDIFLPILISTFISTVSGLFIVAAYQKINILDKTVLGTLAGLFIIVGS
                     ILYFFSKATQHQIALYSNFIASFVLISIIISFILAAMIHKVNVYDAFIEGAKDGFQVA
                     IGIIPYLIAILVAIGMFRAAGAMDMLIRGFTWFFGLIGWNTDFVGALPTALMKPLSGS
                     GARGMMVDAMKLYGADSFVGRLSCIIQGSTDTTFYILAVYFGSVGIKNTRYAVICGLL
                     ADFAGIVAAILIGYLFFH"
     gene            2822..4144
                     /locus_tag="PLD44_08040"
     CDS             2822..4144
                     /locus_tag="PLD44_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOF99314.1"
                     /translation="MNEIIEYIIKFLLGDNVSDDIVQKIGYTDNEEEFGRYRLVIYSS
                     GFFKPEMYGSIHSIPSLPLKIWDDTPLLYGEPEIEEVGNVRIIHADIIASTYFLISRY
                     EEMVRRGCRDVHGRFYGKESLSYKAGFIDRPLVEEYGKLLRQQLRELGIDIPEPEKKI
                     KKIFLTHDVDQLAHYRRFRGMMGAVARGIKSPKKRAEARRAIKTFFKGIRFDPWYTFP
                     WLFKIDQSLTEAIGQERCKTVVFIKAGGGNKKEDKPNSNLAHPDYKNFIRYCKRKNIV
                     IGLHASYEAGRYPNLIAEEKKKLEHAAKTKITYNRHHFLCSREPEDMEALIEAGLKDD
                     FTLSYADMAGFRLGTCRPVQWINPATRQLRPLILHPFTIMDSTLSDKRYMNMNVFDAY
                     EYCVQLINNVENYGGEFVILWHNTMVEKKSPSYHRKLYKNIIDFLKTK"
     gene            4146..>5234
                     /locus_tag="PLD44_08045"
     CDS             4146..>5234
                     /locus_tag="PLD44_08045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOF99315.1"
                     /translation="MQKIAIVCVTNDLVTDQRVHKTCLTLQKCGFFTIEVGRMLPESL
                     PLQRPYVTKRLKLCFRKGPQFYAEYNIRLFFYLLFAKVDLIYANDLDTLPAAFLAAKI
                     RRKKIIYDTHEYFTETPELVNRPKVQAVWEKIEHFIFPKLSDIITVNDSIAELYKKKY
                     NKNLQVVRNIPPTYLPPRLKTRKELGLPENKHILILQGTGINKDRGAEEVISAMQYLQ
                     NCILLIVGSGDVLRTLQKMTMELQLEDKVIFKPKMPFEELRQYTCNSDLGLAVDKDTN
                     LNYRFSLPNKLFDYIHAGIPVLASNLPEIKKIINRYDIGYFLENHDPKNIASTIERIF
                     ENDERYKILKKNTEKARKELCWENEENNL"
BASE COUNT         1625 a          963 c         1035 g         1611 t
ORIGIN      
        1 acaaagaaaa caaaattctt ataaaaaaac aaaaaatcag ataattttct ccaaagaaag
       61 taggacaaga agaaaaacta atccccaaaa aattgctatt gaatttaaat ctctactcga
      121 tttgattagt taaaatttat atctttgcat ctcattcctg aaattattag ttctattaaa
      181 gattaaaaaa atgaacagga aaaagaataa acgaattttt ggatatccac gaaatatttt
      241 ttttaccgga ttaaccagtt ttctcacgga cacttcatta aagatggtct attcggtcat
      301 gcccatgttt ttgctttcca ttggggcatc aaaaaccagc ttatcgctta ttgaaggagt
      361 tgctgaaagt actgcttcac tgatcaaaac aattacgggt ttttggagcg acaaaatagg
      421 caaaaacaaa ccatttttac tgattggcta tggactttcg gctgttatta tacctttgta
      481 ctcgctggtt gtttcaccca ttcaggtgct gattcttcgt ttcgtggaaa gagtggggaa
      541 aggcattcgc acagctccac gcgacagcat tgtagccggt tcggtagaaa atggcgaaac
      601 aggaaaaagt ttcggactgc aaaaagctat ggacaacagc ggagccattt taggcccact
      661 catagcattt atcttgcttt cggcattcaa agacaattac agattgattt ttttgctcgc
      721 cggcattcct tccatattgg gaattttggt gataattttc ggcataacgg aggctaagaa
      781 aaataaggaa agtttggtga aaaaatttcg ttttggcgat ttttccaaaa aattttattt
      841 ctttctggct atagttttta tttttacact tgggaattca accgacgcct tgctgctggt
      901 aaaagccaac gaaataggaa taaaagttgc cttcattccg cttgtttatt tactcaccaa
      961 cgtggtttcc gtattgctgt ctattccagt tggcagttta tccgacagag tggggaaaga
     1021 aaaaattttg attgtcgggt atataatata tgctttggtt tattttggtt ttggagcttc
     1081 cagtcatatc gtggtaattg cttcactctt tgccttatat ggagtctatt ctgctgccac
     1141 tgatggcata cagaaagcat ttgtttcgga cattgttgat gagaataaaa aaggaacggg
     1201 tttggggctc tacaatgcat tattgggaat aaccttgcta cctgccagcc ttatagccgg
     1261 catactgtac gacaaagtaa attcgcatgt tcctttttat ttcggggcgg taactgcttt
     1321 aattgctgct gtattaatgt tttggtttca gagaagcttt aaaaagcaag ttgttgaatg
     1381 aaaaatcaaa gatttaatct gattttgtat tttaatgctg cctccacagt caaaaagagt
     1441 gcaaatcgga aaaatcttgt aattttgcaa gttgtcggaa tatttaaaaa tccggttttc
     1501 cacattaagc ccgaaaaact ttaaaagatg gtattgaatt acatttggat aggttttttt
     1561 ctgattgcat tcgttattgc atgcattcaa ctgttggttt tcggaaatgt tcaggttttt
     1621 tccgacatgc tcaattcaac tttcgattcg gccaagtcag gttttgaaat ttcattagga
     1681 cttacaggcg tattgtcgct gtggctcggt ataatgaaag ttggcgaacg cggtggcgta
     1741 attcgattgt tttctaaggc tgtggcgcct ttttttgaaa aaatttttcc tgatcttccc
     1801 aaaaatcatc ccgcttatgg cagcatgttc atgaatattg ccgccaacat gttgggactc
     1861 gacaatgcgg ctactccatt agggctaaaa gccatgaaag aaatgcagga aaccaatccc
     1921 gtaaaagata ctgcttccaa tcccatgatt atgtttctgg tgctgaatac atcaggattg
     1981 acattaatac cggtaaccat catggtttac agggctcaaa tgggagcaac aaacccttcc
     2041 gatatttttc ttcccatcct aatttcaaca ttcatttcca ctgtaagtgg tttatttatc
     2101 gttgctgctt accaaaaaat caacattctg gacaaaaccg ttttaggaac tttggccggt
     2161 ttatttatta ttgtaggcag tattttgtat tttttcagca aagctactca acatcaaatt
     2221 gctttgtatt caaatttcat tgccagtttt gtactgattt ccataatcat ttcttttatt
     2281 ttggcagcca tgattcacaa ggtaaatgtg tatgacgctt ttatcgaagg tgcaaaagac
     2341 ggatttcagg tagcaatagg aatcatacct tatttaattg ccattttggt tgccattggt
     2401 atgtttcgtg ctgcgggagc tatggatatg ctgattcgtg gtttcacgtg gtttttcgga
     2461 ctgattggat ggaataccga ttttgttggt gccttgccaa ccgctttgat gaaaccgctg
     2521 agcggaagtg gagcacgcgg tatgatggtc gatgccatga aactatatgg ggccgactcg
     2581 ttcgttgggc ggttgtcgtg tatcattcaa ggctcgaccg atacaacttt ttacattttg
     2641 gctgtatatt ttggctcagt aggaataaaa aacactcgat atgccgttat ttgtggactt
     2701 ttagctgatt ttgcaggtat agtagctgca attttaatcg gttatctgtt ttttcattag
     2761 tttttctatt agcaacttga tatttgataa gtgaacagat ttttttgaaa ataatttttc
     2821 catgaacgag ataattgaat atattattaa attcctgttg ggcgacaacg tatcggacga
     2881 cattgttcag aaaatcggat ataccgacaa tgaagaggaa tttggtcgtt atagactggt
     2941 tatttattct tccggatttt tcaaaccgga aatgtacggc tcaatacatt ccattccatc
     3001 cttgccatta aaaatttggg acgatactcc tttgctttat ggagaaccgg aaatagaaga
     3061 agtaggaaac gtacgtatta ttcatgccga tataattgca agcacctatt ttcttatttc
     3121 gcgttacgaa gaaatggtac gtcgtggatg ccgcgatgta cacggacgat tttacggaaa
     3181 agaatcgctt tcctataaag ccggcttcat tgaccgaccg ttggtggaag aatacggaaa
     3241 acttttgcgt caacagttgc gtgaattggg tattgatatt ccggaacccg agaaaaaaat
     3301 caaaaaaatt tttctaactc acgatgtcga ccagctggct cattacagac gctttagggg
     3361 aatgatggga gccgtagctc gaggtataaa atcgccaaaa aaacgtgcag aagcacgaag
     3421 agccataaaa acatttttca agggcatccg attcgaccct tggtacactt tcccttggct
     3481 tttcaaaata gatcaatcac tgacagaagc tatcggccaa gaacgatgca aaacggttgt
     3541 atttatcaaa gcgggaggcg gaaataaaaa ggaagacaaa ccaaactcca acctggcaca
     3601 ccccgattac aaaaatttta ttcgctactg taaaagaaaa aatattgtta ttggccttca
     3661 tgcaagttac gaggcagggc gatacccaaa tctgattgca gaagaaaaaa agaaactcga
     3721 acatgcagca aaaaccaaaa ttacttacaa ccgacatcat tttctgtgtt cgcgcgaacc
     3781 ggaggatatg gaagcactta tagaggcagg cttaaaggat gactttacac tatcatatgc
     3841 cgatatggcg ggattcaggc tgggaacttg tcggcctgtt caatggatta atccggccac
     3901 ccgacaactc agaccgctga tcctccatcc ttttaccatt atggacagca cattgagtga
     3961 taagcgctac atgaacatga atgtatttga cgcttacgaa tattgcgttc aattgattaa
     4021 caacgtagaa aattacggcg gtgaatttgt cattctttgg cacaatacta tggtagaaaa
     4081 aaaatcgccc tcctatcata gaaaattata caaaaacatt atcgattttc tgaaaacaaa
     4141 gtaagatgca aaaaattgcc attgtatgcg taacaaacga tttggttacc gatcaacgcg
     4201 tacacaaaac ctgcctgact ttgcagaaat gcggtttttt taccatcgaa gttggcagaa
     4261 tgttacctga aagtttacca ttgcaacgtc cttatgttac aaaacgactt aaactttgct
     4321 ttcgcaaagg gccgcaattt tacgctgaat ataatatcag gctgtttttt tatttacttt
     4381 ttgccaaagt tgacttgatt tatgcgaatg atttggatac acttccggca gctttcttgg
     4441 cagccaaaat tcgcagaaaa aaaataattt acgatactca cgaatatttt acggaaacac
     4501 ccgaattggt gaatcggcca aaagttcaag ctgtatggga aaaaattgaa catttcatat
     4561 ttccgaaact ttcggatata atcacagtaa acgattcgat tgcagaactt tacaaaaaaa
     4621 aatacaataa aaatctgcaa gtggttcgca atatccctcc tacctatctt cctcctcgat
     4681 tgaaaaccag aaaagaattg ggtttacctg aaaacaaaca tattttgata cttcagggaa
     4741 ccggtatcaa taaagatcga ggagcagaag aagtgatttc tgcaatgcaa tatttacaaa
     4801 actgtatatt actgatagta ggaagtggcg atgtgctgcg gactctgcaa aaaatgacaa
     4861 tggaactgca attggaagat aaagttattt ttaagcccaa aatgccgttt gaagaattac
     4921 gtcaatatac ctgcaacagc gatttgggac tggctgtcga taaagatacc aacctgaatt
     4981 accgattttc tttaccaaat aaacttttcg actatattca tgccggcatt cccgtgctcg
     5041 cttccaactt accggaaata aaaaaaatta ttaaccgtta cgatatcggc tattttttgg
     5101 aaaatcacga tccaaaaaac attgcttcca ccattgaacg tatttttgaa aatgatgaac
     5161 gctacaaaat actgaaaaaa aatacggaaa aagctcgaaa agaattgtgc tgggaaaacg
     5221 aggaaaataa tttg
//
LOCUS       DAOSIB010000107         5193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_14261_length_5193_cov_12.327949, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000107 DAOSIB010000000
VERSION     DAOSIB010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14261_length_5193_cov_12.327949"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(541..1221)
                     /locus_tag="PLD44_08050"
     CDS             complement(541..1221)
                     /locus_tag="PLD44_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOF99316.1"
                     /translation="MKKQVSPTFMVAGLLFTACLIAANIVAQKTITIFGLSATAALLV
                     FPISYIVNDLIAEVWGYRKARFIIWFGFLMNFFVVLIFQLSILIPPNQFYPHQDAFSQ
                     VLGSSLRLTFASFISFLLGSFVNAYIMSKMKILQKGKGFSVRAIVSTFAGEGIDSLFF
                     FFIAFVGLMPLKNIFELTVHIALLKTAYEILVLPLTIYLVKKIKEKEGVDVFDEDVNY
                     NPFRIKEI"
     gene            complement(1322..1732)
                     /locus_tag="PLD44_08055"
     CDS             complement(1322..1732)
                     /locus_tag="PLD44_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99317.1"
                     /translation="MSNLSSIRDRVSEYLLLKGISKYRFYQETGLSNGFLDKAGSITS
                     DNCEKICYAYSDLDPEWLLTGRGNMLRTYDKIDEENDRKSASVEVLVQKIVELSSENT
                     LLKKENENLKKKIVGYKPLSEEDLKDNIAAEPGI"
     gene            1898..2083
                     /locus_tag="PLD44_08060"
     CDS             1898..2083
                     /locus_tag="PLD44_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99318.1"
                     /translation="MKNETDNPFSIPRHGYITKLAKMCNCSRKTVTRALFHGYTGPKA
                     NKVIETYKKHFQGKMKY"
     gene            complement(2121..2522)
                     /locus_tag="PLD44_08065"
     CDS             complement(2121..2522)
                     /locus_tag="PLD44_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOF99319.1"
                     /translation="MRTTALIEKGKDGTFGIFTPDIETTIIGEGTTVAEAKADFENSV
                     QEIISAYREAGDELPAELKNIKFVYKYDIASLFDYYSFINVSKFAQVAGINASLMRQY
                     RMGQYISENQMRKIETTLHNVGKELSSIKLV"
     gene            complement(2547..2729)
                     /locus_tag="PLD44_08070"
     CDS             complement(2547..2729)
                     /locus_tag="PLD44_08070"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOF99320.1"
                     /translation="MKWNELRRIAEKNGWYLYRNGSRHDIYRHLEKDYPIQIGRHGSE
                     EIKTGTFNKLKKQIGF"
     gene            complement(2944..3339)
                     /locus_tag="PLD44_08075"
     CDS             complement(2944..3339)
                     /locus_tag="PLD44_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOF99321.1"
                     /translation="MKKVKVFIERASDGYYSAYMPDSPLNFGATGEGSTATEAQEDFL
                     NVVNDYVKNGETLPEGAEFEFNYDVASFLEYYRTRLTLAGLSRITGVSQGQLSHYVTG
                     HRKPSARTVEKIQQSLHRFAEDLQQIHFI"
     gene            complement(3381..3566)
                     /locus_tag="PLD44_08080"
     CDS             complement(3381..3566)
                     /locus_tag="PLD44_08080"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOF99322.1"
                     /translation="MKYSELHRLLQKAGCYQTGEQRVGHPGRYSPITGKKFTTSNHLS
                     REVATGTLKSIKRDAGI"
     gene            complement(3734..4159)
                     /locus_tag="PLD44_08085"
     CDS             complement(3734..4159)
                     /locus_tag="PLD44_08085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOF99323.1"
                     /translation="MQKVILDTNVVVSALIQKNYPFLIIDELFIEDKFQLCISDDLLK
                     EYFDVLARPKFNKFPNFATKAKEVLTEIVLKARFYYPTVKLDILSDKDDNVILELAYE
                     SKADYIITGNTNDFTLSEFKGTKIISPKDYWEMYNPNKF"
     gene            complement(4140..4370)
                     /locus_tag="PLD44_08090"
     CDS             complement(4140..4370)
                     /locus_tag="PLD44_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99324.1"
                     /translation="MRTLNISISELEFNKFGLTDDTLSFSELLEIINKELIKQNLRKS
                     VYFAEKYKLSKMTMSEITDEVKAVRRDAKGNS"
     gene            complement(4524..4919)
                     /locus_tag="PLD44_08095"
     CDS             complement(4524..4919)
                     /locus_tag="PLD44_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005651848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5053 domain-containing protein"
                     /protein_id="HOF99325.1"
                     /translation="MTTQEKFEQLKNREMNAGTESEYREIQVAMEKLADADPEGFEQA
                     VLASMRRTRQKAQNLRIKEQMEAVSEIISMSYIAKTYFNKTKSWLSQRINELNVNGKP
                     ASFTDEEIETLNYAFKDISKKIGAFSVSR"
     gene            complement(4964..5119)
                     /locus_tag="PLD44_08100"
     CDS             complement(4964..5119)
                     /locus_tag="PLD44_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99326.1"
                     /translation="MKKYISKQRREVEKELLMYLRNYRFFRSRFTKTDELDKLINDIV
                     EELKSDI"
BASE COUNT         1512 a         1031 c          903 g         1747 t
ORIGIN      
        1 cattgattaa taaagttgac gagatcaaaa gtaaagataa aatgtatttc atacgtttaa
       61 atttcatacg tttaaattta ttgaacttga ggctttgccc caaaccctac ttcatttttt
      121 tgtcttgata caaaaaaatg aagcaaaaaa aaatcaagac tgtgcctgct tcgctcaatc
      181 tgcatcatcc gtgtgctact ctttcgaggt cgctatgtga acttcgtaga agttccacgt
      241 gcataacccc caattttaat tgggggcaac tgaacccacg accacctaaa cgctgtaggc
      301 gtttaacaaa ctaagtttag ggaaactctt acagagtttt atttaccatt ctcgaccttt
      361 ccaccccgga tgaatccggg ggttattcac gtgtttgtcc tacggacaat ttcgatattt
      421 ttaccctatt ctcacttatt tttcggctca ccggacgagg tcggtttgca tccaaccagt
      481 atatgtaaaa agtacaaatt tactttttga gcactatttt acaaattaca tatatctttt
      541 ttatatttct ttaattctga aaggattata atttacatcc tcatcaaaaa cgtccacacc
      601 ctccttttcc ttgattttct tgaccaaata aattgtcagc ggcaggacca aaatctcata
      661 agctgtttta agtaaagcaa tgtgtacagt cagttcgaaa atattcttca gcggcatcaa
      721 gcctacaaaa gcgatgaaga aaaagaataa agaatctatg ccttctccgg caaaagtgga
      781 aacgatggca cgaacagaga agcccttgcc tttttgtaag attttcattt tgctcatgat
      841 ataagcattc acgaacgacc ccaacaaaaa agaaataaaa cttgcaaagg tcaggcgtaa
      901 ggaagagcct aatacctgac tgaaagcatc ctgatgtggg taaaactgat tgggtggaat
      961 gaggatgctc agttgaaaga tcaatactac gaaaaagttc atcaaaaagc caaaccaaat
     1021 gataaagcgg gcttttcgat aaccccacac ctcagcaatc aggtcgttta cgatgtacga
     1081 gattgggaat accaacaacg cagccgtagc actgagtccg aagatggtaa tggtcttttg
     1141 agcgacaatg ttggcggcaa tcagacaagc tgtgaagagc agtcctgcca ccataaaggt
     1201 aggcgatact tgttttttca tttttcttgt tttttacgtg gtttatcaag cacacgttcc
     1261 ggtaatcaca caacagccac cggaatcggc tgcaaaggta cattaaaata ttgtcttgta
     1321 gctatattcc tggttctgct gcaatattat ctttcaaatc ttcctcacta aggggtttat
     1381 aaccaactat tttcttcttc aggttttcgt tttctttttt aagtaaggtg ttctcggagg
     1441 acaattccac tattttttgt accaacacct caacagaagc ggattttctg tcattctcct
     1501 catcgatctt gtcgtaagtt ctcaacatat tgcctcttcc tgtcagcaac cactccggat
     1561 ccaaatccga ataagcatag catattttct cacaattatc actggtgatt gaacccgcct
     1621 tatctaaaaa cccgtttgac aaacctgtct cctgataaaa gcgatattta gatatccctt
     1681 ttaaaagaag atattctgag actctatcac gaattgagga aagatttgac ataaacaact
     1741 taaaaaaaca tcgaaaaatt gcgcaataga aaataagcta ctatctttgt aagcggcaac
     1801 gaactttatg ccccaaatgg gacatttacc cgcaaatgta tgaaatattt atcgaatatt
     1861 tatcgtgttt acgtacaaac aacttaacca aattgagatg aaaaacgaaa ctgacaatcc
     1921 tttctctatt ccccgtcacg gatacattac caaactggcg aaaatgtgta attgcagtcg
     1981 caaaaccgtt acgcgtgcgc tatttcatgg atataccggt cccaaagcca ataaagtaat
     2041 agaaacttat aaaaagcatt ttcaggggaa aatgaagtac taactcttat gaaatggata
     2101 cattgcgaag ccgattgttt ttatacgagt ttaatagacg aaagctcttt acctacgtta
     2161 tgaagcgttg tttctatttt tctcatctga ttttcgctga tatattgacc catacgatat
     2221 tgacgcatta aagatgcgtt tataccggct acctgtgcaa atttagaaac attgataaaa
     2281 gaatagtaat caaataaaga tgctatatcg tacttatata cgaacttgat gtttttcaac
     2341 tctgcaggca actcatcgcc ggcttcacga tatgcactga ttatttcctg cacggagttt
     2401 tcaaagtctg cttttgcttc ggctacggtg gtaccttcac ctataatggt agtttctata
     2461 tccggcgtaa atataccgaa agtaccgtct tttccttttt ctattagtgc agttgttctc
     2521 atgttgatca ttatttttag aatggattaa aaccctatct gcttttttag tttgttaaat
     2581 gtgccggttt ttatttcctc ggaaccatgc cgcccgattt ggatggggta atctttttcc
     2641 agatgtcggt agatatcgtg cctgctaccg tttcggtaaa gataccagcc gttcttttcg
     2701 gctatccttc ttaattcatt ccatttcatt tcatagcttt tagattacgg cacaaatata
     2761 taacgttttt gttatatatg caaatatact gattaaaaag cctttttttc aggataatgg
     2821 ggtggcgtgt ctccgtgttc cgagttccgc ggtgcggggt gcgggatcac gacgaggttg
     2881 gtgactcgca gattaacgca aatcgcaatt gacagagtcc aattattatt cggtgtcaat
     2941 caatcaaata aaatggatct gctgtaaatc ttcagcaaat ctgtgtaatg attgctgtat
     3001 tttttctact gtacgtgcac ttggcttacg atgccctgtt acataatgac ttaattgtcc
     3061 ttgtgatacg cctgtgatgc gtgataatcc ggcaagtgtt aagcgggtgc ggtaatactc
     3121 taaaaacgag gctacatcgt agttgaattc aaactcagct ccttccggta atgtttcgcc
     3181 gtttttcacg taatcattta ccacgtttaa aaaatcttcc tgagcttcag ttgccgtact
     3241 accttcacct gtagcaccga aatttaacgg agaatcgggc atataagcac tataatagcc
     3301 atctgatgca cgttctataa atacttttac ttttttcatt gctatttcgt ttttttaatt
     3361 cttacttcaa aaagtgggga ttaaatcccg gcatcccgct taattgattt taatgttcct
     3421 gttgctacct ctcgcgagag atgattgctt gttgtaaatt ttttacccgt tatcggactg
     3481 taccgccccg gatgtccgac tcgctgctcg cctgtttgat agcaaccggc tttttgcaac
     3541 agcctgtgta gctcgctgta tttcatattt cttttattag attgattaac gacacaaagt
     3601 taatgatatt agttttaata tcaaaatctt tatttggggt gcaagttccg gggtggaaag
     3661 gtcgagaatg gttataaaac tctcagtaac cacgttactt tggatagcgg gggcaaaatc
     3721 cgaatgttcc tatttaaaat ttattcggat tatacatttc ccaataatct ttcggactaa
     3781 ttattttggt acctttaaat tccgacaaag taaaatcgtt tgtatttcct gtaataatgt
     3841 aatcggcttt actttcatag gcaagttcca aaattacgtt gtcgtctttg tctgaaagta
     3901 tgtctaattt aactgtggga taatagaaac gagcttttaa aactatttcg gttaacactt
     3961 cttttgcttt tgttgcaaaa ttggggaatt tattaaattt aggtcttgca agtacatcaa
     4021 aatactcttt caacaaatca tcagagatac aaagttgaaa tttatcctca ataaacaact
     4081 catcaatgat aagaaacgga taattctttt gaattaaagc cgatacgaca acatttgtat
     4141 caagaattac cttttgcatc tcgtctcacg gctttaactt cgtccgtaat ttcactcatt
     4201 gtcattttcg acaacttgta tttttccgcg aaataaacag attttctaag gttctgtttg
     4261 atcagctctt tattgattat ctccaaaagc tcactgaaac tcaatgtgtc atctgtcaat
     4321 ccaaacttgt taaattcgag ttccgatatg gatatattta atgttctcat tttatttaaa
     4381 acttagtgtt ccaacgcaaa gataaagatt ttatttattt cgtacaatga tatgaattct
     4441 cactccaata aatgctatct cctataaatg tacttgatta gttgcaggac gcaaaccgac
     4501 cttgcctggt gagccaaaaa aatttaacga gaaacactaa atgcaccaat tttttttgat
     4561 atgtctttaa aggcataatt aagtgtttcg atttcttcat ctgtaaatga agccggctta
     4621 ccattaacgt taagttcatt gatacgttga ctaagccacg atttggtttt attaaaatac
     4681 gttttggcta tatatgacat ggatatgatt tcggaaactg cttccatttg ctcctttatt
     4741 ctcaaatttt gagctttttg gcgggtgcgt cgcatgcttg ccaatacagc ttgctcaaat
     4801 ccttcgggat ccgcatcggc aagtttctcc atggcaactt gaatctcccg gtattcggat
     4861 tctgtaccag cattcatttc acgatttttt aactgttcaa atttttcttg cgttgtcatc
     4921 gttggtagtt ttttaacatg cccttccgcg ggcgcttgtt ttatcaaata tctgatttta
     4981 attcttctac gatgtcgttg atcaatttat caagttcatc tgttttagta aaacgtgaac
     5041 ggaaaaatcg gtagtttcgt aaatacatca acaattcttt ttccacttca cgtcgttgct
     5101 ttgaaatgta ctttttcatt aagttttagg tttagttgtt ttttctcttt gacgctacaa
     5161 acgtaataaa cttttgttta ttaaacaagt aaa
//
LOCUS       DAOSIB010000108         5099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_14617_length_5099_cov_10.863600, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000108 DAOSIB010000000
VERSION     DAOSIB010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14617_length_5099_cov_10.863600"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(418..491)
                     /locus_tag="PLD44_08105"
     tRNA            complement(418..491)
                     /locus_tag="PLD44_08105"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(455..457),aa:Ala,seq:ggc)
     gene            578..892
                     /locus_tag="PLD44_08110"
     CDS             578..892
                     /locus_tag="PLD44_08110"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HOF99327.1"
                     /translation="MLIFNTTYLVENEFYPQWINWVQESLIPFMMSTAGFSEPQIAKV
                     LSGESVKETSYSVQFKISDKNALNDWMRQYGEDFARLCSVNFGNHVLFFSTMLELIND
                     DK"
     gene            882..1466
                     /gene="ruvC"
                     /locus_tag="PLD44_08115"
     CDS             882..1466
                     /gene="ruvC"
                     /locus_tag="PLD44_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOF99328.1"
                     /translation="MINDQIILGIDPGTTIMGYGVLNIRAHQPELIAMGILDLKKYAN
                     HYVKLQRIFARTLSLIDEFHPACMAIEAPFFGKNVQSMLKLGRAQGVAMSAALYRDIP
                     ITEYAPLKIKMAITGNGNASKEQVADMLRRFLHIPQENMLPQLDATDGLAAAYCHFLQ
                     LGKPESQKVYSGWKDYISKNQEKVKNPIQKKREK"
     gene            1558..2658
                     /locus_tag="PLD44_08120"
     CDS             1558..2658
                     /locus_tag="PLD44_08120"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HOF99329.1"
                     /translation="MKKSFLLVLCLLFLFQSAEGKIYRFLIGTYTQNTVSEGIYSLSF
                     DAESGVCKIQLAATNVDNPSFLAFSPDKNFVYAVNESGTESKIASFRFDATSGKLSFI
                     NRVEAIGSDPCFITTSKRHIITANYSSGNACVFKRNSDGSIGETVQKIQFNGHSIDSM
                     RQTSSHVHQTIFSPNGHFLLINDLGTDLIHSFRYVPNRSENCLLPYDSLKVKPGSGPR
                     HLTFSKDGKFVYLLHELDGTISIIGFNKGKLLFYGENSVVTNKKLKTGAADIHISPDG
                     KFLYATNRGEANNITCFKILKNGLLEFVEQNSTQGKGPRNFAITKDGHFLLVGHQYSD
                     EIVVFKRDRENGKLMDTKIRIKIGAPVCLLEY"
     gene            complement(2668..4941)
                     /locus_tag="PLD44_08125"
     CDS             complement(2668..4941)
                     /locus_tag="PLD44_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOF99330.1"
                     /translation="MADEKSIQEQEREEERMIQQEFEGLLEDYRNSNHRQKVEIITKA
                     FNFAKTAHKGMKRRSGEPYIMHPLAVARIVVKEIGLGSTSICAALLHDVVEDTEYTVE
                     DIRHHFGDKIAQIVEGLTKISGGVFAEKASEQAENFRKLLLTMSDDIRVILIKIADRL
                     HNMRTLGSLPPAKQYKISGETQYIYAPLAHRLGLFRIKTELENLSFKYEHPDIYKSIE
                     NKLLMDEEARNAFYKEFSAPIREKLTEMGYEFTLKERVKSVYSIWHKMSTRNIPFEEV
                     YDILALRIIFKPKEGMPEKDQCWMIYSAITALYKPHPERIRDWLSTPKANGYEALHVT
                     VMGPNGRWVEVQIRTERMNEIAEKGLAAHWKYKLGENNESELDNWLKTIKEVLEHPDT
                     DAINFLETFKLTLFASEIFVFTPKGEIKTIAQGATALDFAFMIHSDLGMHCIGAKVNH
                     KLVPLNHRLQSGDQVEILTSQKQTPKEEWLNYVTTAKARSKILELLKKEEKNIAAEGQ
                     KMIENALAEINLPASNENIMKILQHYNLTQRNELYVQAGKGALNLDDIKKVIFKQKTQ
                     NVFSKYLKIPFIGSSSSDNKEIIIPTQGIDRKATLKLTEENTGKIFKLAECCHPIPGD
                     DVLGYLDENGIIYIHKRQCPVALKLKSNFGERILSAEWATHKILSFEETIEIKGIDRK
                     GILIEILKVISEAYGVNISKINIETESGIFIGKFQVLVHDTEELNNMCENLTKIPEVQ
                     SVQRIEQTNLQPQEQKT"
BASE COUNT         1479 a          885 c         1009 g         1726 t
ORIGIN      
        1 atacacaaat aaggagaata gttaactttt tcataagctg agttttaata gtaataatta
       61 tggtttaatt taattttaca aatttttttg ttcaataaaa gatattatat aatgctggga
      121 atcgtgtttt ttttaaaaat aaggagaata aaaactaaat tatatctatt gatattcaat
      181 tgtttaactt tgtgatattt taataataga tattgaaatc aagcaatttt ccagttttac
      241 accttttatt tttttaagga attccttttt atacaaatgt atttagtaat tgacgacatt
      301 ggtctgcaaa aggtaaaaaa aaataagcaa aaggtaaaaa agtgataaat attgcttttt
      361 tcaatggatg gaaaagaggg aaaataaaaa aagcttacca aagataagct tttcgagtgg
      421 agattaccgg agtcgaaccg gttacctcga cactgccagt gtcgcgctct agccaaatga
      481 gctaaacccc caaaatgtat attttttcac tgcaaagtta aagaactttt tcaaattatg
      541 cttacttttg ttcaaattat tccataaaaa attacaaatg ctgattttta atacaactta
      601 tttggttgaa aacgaatttt atcctcagtg gataaattgg gttcaagaat cattgattcc
      661 atttatgatg agcactgccg gtttctcgga gcctcaaatt gccaaagtgc tttccggtga
      721 aagcgtgaag gagacttcct attcggttca gtttaaaatt tccgataaaa atgctttaaa
      781 cgattggatg aggcaatacg gagaagattt tgcaagatta tgttcagtta actttggaaa
      841 ccatgttctt ttcttttcaa ccatgctcga attgattaat gatgataaat gaccagatta
      901 tattgggtat tgatccggga actaccatta tgggatatgg agtgctgaat atcagggctc
      961 atcaacccga attaatcgct atgggaattc tggatttaaa aaagtatgcc aatcattatg
     1021 taaaattgca acgtattttt gctcgaacat tgtcgctcat agatgaattt catccggcct
     1081 gtatggccat agaagctcct tttttcggga aaaatgtgca gtcgatgctg aaacttggaa
     1141 gggcgcaggg cgtagccatg tcggctgctc tgtatcgtga tattcccata acggaatatg
     1201 caccgttgaa aataaagatg gctataacgg gcaacggaaa tgcttcaaaa gaacaggtcg
     1261 ccgatatgtt gcgcaggttt ttgcatattc ctcaggaaaa catgcttcct cagctggatg
     1321 ctactgatgg cttggcagca gcctactgtc attttttaca gttgggaaaa cccgaatctc
     1381 agaaagttta ttccggctgg aaagattata tttcaaaaaa tcaggaaaaa gtgaaaaatc
     1441 ccatacagaa aaaaagagaa aaatagattt tttagtcaag aaaaaaaacg tttcgagctt
     1501 tgaaacatat ttatataaga tattgattaa caatatccta atttaaaaat attcattatg
     1561 aaaaagtcat ttttattggt tttgtgtttg ttgttccttt ttcagtcggc agaaggaaaa
     1621 atatatcgtt ttttgatcgg aacttacacg cagaacactg tgagcgaagg aatttattct
     1681 ttaagttttg atgctgaaag tggagtttgt aagattcaac ttgctgcaac taatgtggat
     1741 aatccaagtt ttttggcatt ttcgcccgac aaaaattttg tttatgccgt caatgagtcg
     1801 ggtactgaga gtaaaatcgc atcttttcgt tttgatgcca cttcaggcaa actgtctttt
     1861 attaaccgtg tggaagcaat tggctcggat ccctgtttta ttactacttc aaaacgacat
     1921 attattacag ccaattattc aagtggcaat gcttgcgttt ttaagagaaa ctcagatggt
     1981 tcgataggtg aaacggttca aaaaattcag ttcaatggac acagcattga ttccatgcgt
     2041 cagacaagtt ctcatgtaca tcaaacgata ttttcaccaa acggacattt tttattgatc
     2101 aacgatttgg gaaccgattt gatacattct tttcgttatg ttcccaatag atcagagaat
     2161 tgtcttctac cttatgacag tttaaaagtt aagccgggta gtggacctcg acatttgact
     2221 ttctccaaag acggaaaatt tgtttatttg cttcacgaat tggatggaac aatttccatt
     2281 atcggattta ataaaggaaa attgttgttt tatggcgaaa attctgtagt aacgaataaa
     2341 aaattgaaaa ccggagcggc cgacattcat atttctcccg atggtaagtt tctgtatgcc
     2401 accaatcgtg gagaagccaa caatattacc tgttttaaaa tactgaaaaa cggtttgttg
     2461 gaatttgttg agcaaaattc aactcagggc aaaggaccgc gtaattttgc aattaccaaa
     2521 gacggccatt ttttgttggt tggccatcag tattccgacg aaattgtggt ttttaaaagg
     2581 gacagggaaa acgggaaact gatggatacg aaaatacgga taaaaattgg agcgcctgtt
     2641 tgcctgctcg aatattgaga ggttttatta ggttttttgt tcctgaggtt gcaggttggt
     2701 ttgttcgatt cgttgaacag actgaacttc gggaattttg gtcagatttt cgcacatgtt
     2761 gttgagttct tcggtgtcgt gtaccaacac ttgaaatttg ccgataaaaa ttccactttc
     2821 cgtttcgata tttattttgc tgatgttgac gccgtaagct tcagaaatta cttttaaaat
     2881 ttcgatcaga attcctttgc ggtcaattcc ttttatttcg atagtttctt cgaaagatag
     2941 aattttatgt gtggcccatt cggcagaaag aattctttcg ccaaagttac tttttaattt
     3001 caatgccacc ggacattgtc gtttgtgaat gtagataatg ccgttttcgt cgagatagcc
     3061 caacacatca tcacccggaa tgggatgaca gcattctgcc agtttgaata tttttcccgt
     3121 gttttcttcg gtcagtttca gggtagcctt tctgtctatt ccctgagtgg ggataataat
     3181 ttccttgttg tcggaagaag agcttccgat aaagggaatt tttaaatatt tggagaaaac
     3241 attttgcgtt ttctgtttaa agataacttt tttaatatcg tcaaggttta gagctccttt
     3301 acctgcctga acataaagtt catttcgttg ggtaaggttg taatgctgaa gaattttcat
     3361 gatattttcg ttgctggcgg gcaggtttat ttcggccaac gcattttcta tcattttttg
     3421 accttccgcc gcaatgtttt tttcttcttt tttcaggagt tcgagaattt tgcttctcgc
     3481 tttggctgta gtaacgtaat tcaaccattc ttccttagga gtttgtttct gcgaagttag
     3541 gatttcaacc tgatcgccgc tctgcaaacg gtggttcaag ggcacaagtt tgtgattcac
     3601 tttggctcca atgcagtgca tgcccaaatc ggaatgaatc atgaaggcaa agtcgagagc
     3661 ggtggctccc tgagcaatgg ttttgatttc tccttttggt gtaaaaacaa aaatttcgga
     3721 tgcaaataag gttaatttga aagtttcgag aaaatttatg gcatccgtat ccgggtgttc
     3781 aagtacttct ttaatggttt tcagccaatt gtccaattca gattcattgt tttctcccag
     3841 tttgtatttc cagtgggcag ccaacccttt ttcggcaatt tcattcattc tttccgtacg
     3901 aatttgtact tccacccacc gaccgtttgg ccccattacc gttacgtgaa gtgcttcgta
     3961 tccgttggct ttgggtgtac tcagccaatc tcttatacgt tcgggatggg gtttgtaaag
     4021 tgcagtaatg gcggaataaa tcatccagca ctgatctttt tcaggcatac cttctttggg
     4081 cttgaaaata atgcgaagtg ccagaatatc atatacttct tcgaaaggga tattgcgggt
     4141 tgacatttta tgccaaatgg aatagactga ttttacacgt tctttcaggg taaattcata
     4201 tcccatttca gtcaattttt ccctgatagg agcagaaaat tccttataga aagcatttct
     4261 tgcttcttcg tccatcaaca gcttgttttc gatggatttg taaatatctg gatgttcgta
     4321 tttgaaactg agattttcga gttcggtttt aatgcgaaaa aggccaagtc tgtgggcgag
     4381 tggagcataa atgtattgag tttcgcccga aattttgtat tgtttggccg gaggaagtga
     4441 tcccaaagtg cgcatattgt gcagtctgtc ggcaatttta atcaggatga cgcgaatatc
     4501 gtccgacatg gtgagaagaa gttttctgaa attttcggct tgttcagaag ctttttcggc
     4561 aaaaacacct ccggaaattt ttgtaagtcc ttctacaatt tgtgcaattt tgtcaccaaa
     4621 atgatggcga atatcttcaa cagtatattc ggtatcttct accacatcgt gcaacaaggc
     4681 ggcacaaatg gaagtagagc ccaagcctat ttctttaacc acaatccgtg ccacagccag
     4741 tggatgcatg atgtatggct cgccggaacg tcgtttcatg cctttatgag cagttttagc
     4801 aaaattaaag gcttttgtaa tgatttcgac tttctgccga tgattggaat ttcggtagtc
     4861 ttcgagcaag ccctcaaatt cctgttgaat cattctttct tcttctcttt cttgttcttg
     4921 aatactcttt tcgtcggcca taaacttgca tcctatttta tgcaaatata aataattttt
     4981 tcaattctga tttggctttt ttacaacaaa aactcatttc aatccattat tgaaaacaat
     5041 tttcagattg aaagagttcg acaaagtcaa tcagaatgaa ttttattcta ataacctgc
//
LOCUS       DAOSIB010000109         5065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_14756_length_5065_cov_9.890419, whole genome shotgun sequence.
ACCESSION   DAOSIB010000109 DAOSIB010000000
VERSION     DAOSIB010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14756_length_5065_cov_9.890419"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLD44_08130"
     CDS             <1..327
                     /locus_tag="PLD44_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HOF99331.1"
                     /translation="YCQTLKLKDDATLIEEYKFWHAPENRWPEISEGIKAVGILDMEL
                     YIHGNLLFMIVETPVDFDWDKAFGKLANLPRQAEWEAFMAKFQMADPNAGSAEKWTLM
                     ERIFSL"
     gene            462..1850
                     /gene="fucP"
                     /locus_tag="PLD44_08135"
     CDS             462..1850
                     /gene="fucP"
                     /locus_tag="PLD44_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HOF99332.1"
                     /translation="MKSDTKIPLLKGSDGTNYLIPFILVTSLFFFWGVANNMTDTLLS
                     AFKNIMEMSDFQTSFIQMAFYGSYACFAIPAALLIRKYTFKHGIVVGLSLYALGTFLF
                     YPASQLASYGFYLFALYVLAGGCSILETPANPYILTMGRKENMTRRLNFAQSFNPIGS
                     ISGIIISQIFILSQLSQNKSTMNDAELLAVQHKELSAVTLTYMGLGVVLVALLLIIIF
                     YKKMPATKDTTGLSFGATVGRLLKRKHYTLGVVAQFFYVGAQIGVWSYTIRYVMLALG
                     LVDPVTSEIFPAKVQGFIAEHNLWSSIWQLDENSTAENIGNSFYLFSLILFTVSRFIF
                     TALMRYFRPGMLLAVAATGAIISAAIAIFATGMTGVIGLVAISFFMSLMFPTIYGIAL
                     EGISEAEAKVGGSFLVMAILGGAILTAIQGYVSTTLNSINFAYWIPVVCFIVVGVYGI
                     IANKEEKEFGYE"
     gene            1898..2881
                     /locus_tag="PLD44_08140"
     CDS             1898..2881
                     /locus_tag="PLD44_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOF99333.1"
                     /translation="MKVNFSHSKSKINTLIDAISNDIASGVLKLGDNLPSINETSLKF
                     KISRDTVFKAYKELKRRNLIDSNPMKGYFVKGEVNHVLLLLDTYSPFKQNLYNRFAQN
                     LPKSWKVDLIFHQYNKNLFDTIIRESIGRYSMYVVMNFSNTEFSESLKSIPEGKLLLL
                     DFGDFDKKQYPYLCQNFDQALYDCLSEHKPIISKYKKIVMIFPTENNHPKSSIKFVKE
                     FCSDSNIAFELIEDIYLLNNIEENVLYFIMSHTDLVSIILASKNKNLKIGQDIGILVY
                     NDEPLLEVIQEGIAAISIDFGLLGEKAAQFIKDKMFVQEFLPTRFIFRPSI"
     gene            3006..4844
                     /locus_tag="PLD44_08145"
     CDS             3006..4844
                     /locus_tag="PLD44_08145"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HOF99334.1"
                     /translation="MKATITGQAQQIVQTRKEKSGRLQGDNPKIGIRPTIDGRQGGVR
                     ESLEDQTMRMALNLAKFLSSNLKYSDGTPVECVIADTTIGRVDEAAACAEKFKKEGVS
                     ITITVTPCWCYGSETIDMDPHTIKAVWGFNGTERPGAVYLAAALAGYNQKGLPAFGIY
                     GRDVQDAGDTSIPADVQEKLLRFSKAAIAAASMKGKSYLSIGSVSMGIAGSIVDPNFF
                     QEYLGMRNEYVDMSEINRRVQLGIFDQEEFARAMEWTSKYCKVNEGEDKNDEDKKKTR
                     EQLDKEWEFVVKMTIIIRDLMIGNPKLREAGFLEEALGHNAIASGFQGQRQWTDFMPN
                     GDFSEAILNTSFDWNGIREPFVVATENDSLNGVAMLFGKLLTNTAQVFADVRTYWSPE
                     AVERVTGYKLEGIASNGLIHLINSGSAALDGSGQQTKDGKPAMKPFWEITDEEVQKML
                     DATTWYPADCGYFRGGGFSSKFVTKGGMPVTMARVNLVKGLGPVLQLAEGWTVEIPAE
                     VHKILDDRTDKTWPTTWFVPRLTGKGNFKDVYSVMNNWGANHGAFSYGHIGADLITLA
                     SILRIPVCMHNVEEDKIFRPSAWAAFGMDAEGSDYRACATYGAMYK"
BASE COUNT         1613 a          857 c         1027 g         1568 t
ORIGIN      
        1 tattgtcaga ctttaaaatt aaaggatgat gcaactttaa ttgaagaata taaattctgg
       61 catgcgcctg aaaatcgctg gcctgaaata tcggaaggaa taaaagcggt tgggatcctc
      121 gatatggaat tatatataca tggcaatctg ctttttatga ttgtggagac tcctgtagat
      181 tttgattggg ataaagcttt cggaaaactt gccaacttac cacggcaagc agaatgggag
      241 gcatttatgg caaaatttca gatggcagac ccgaatgcag gttcagcaga aaaatggact
      301 ttgatggaac ggatttttag cttgtgaatt accatttacc atttaccatt taccatttaa
      361 tcatttacta tttacaaatt atagacaaaa atgtaaaact gaaatatgca cagatgcaat
      421 gcattgtgtc tgtatcaaaa aatacaaaca aataaaatat catgaaatca gacactaaaa
      481 ttcctttatt gaaaggcagc gatggtacaa attatttaat tccttttatt ttagttactt
      541 ctcttttctt tttttggggg gttgctaaca atatgactga cacacttctg tctgcgttta
      601 aaaacattat ggagatgtcc gattttcaga catcgttcat acaaatggca ttttatggct
      661 cttatgcatg ttttgcgata ccggcagctt tactaatcag aaaatataca tttaaacatg
      721 gaatagttgt agggctctcg ctttatgcgt taggaacttt cctgttttat ccggcttcgc
      781 aacttgcatc ctacggcttt tatttgtttg cattgtatgt tttagccgga ggttgctcaa
      841 tcctcgaaac tcctgccaat ccttatattt taaccatggg tcggaaagaa aacatgaccc
      901 gcaggttaaa ttttgcccaa tcttttaatc cgattggttc aatttcagga attatcatca
      961 gtcagatatt tattttatca cagttaagcc agaataaaag cacaatgaac gatgccgaat
     1021 tgcttgccgt tcagcacaaa gaattatcag cagttacact tacgtacatg ggcttgggtg
     1081 tagttcttgt agcattactt ctaattataa ttttttacaa aaaaatgcct gccaccaaag
     1141 atactacagg attaagtttt ggcgctacag ttggcagatt gctaaaaaga aaacattata
     1201 cacttggtgt ggttgctcag tttttttatg tcggagctca aatcggcgtt tggtcttaca
     1261 ccattcgtta tgtgatgctt gcactcgggc ttgtcgatcc tgtaacttct gaaatttttc
     1321 cggcaaaagt gcagggattt attgctgaac ataatttatg gagcagtatc tggcaactcg
     1381 atgaaaactc tactgccgaa aatattggca attcctttta tcttttctcg ctgattttat
     1441 tcactgtttc aagatttatt ttcactgctt taatgcgtta tttccgtccc ggaatgttgc
     1501 tcgcagtagc tgctacaggt gcaataattt ctgctgcaat cgcaattttt gcaaccggaa
     1561 tgacaggtgt cattggttta gttgccattt catttttcat gtcgcttatg ttcccgacaa
     1621 tttacggtat tgcgcttgaa ggcataagcg aagcagaagc aaaagtaggc gggtcgtttt
     1681 tggtaatggc tattttgggt ggcgctatac tcactgcgat tcagggttat gtttcgacta
     1741 ctctaaacag tataaatttt gcttattgga tacctgtagt atgttttata gttgttggtg
     1801 tttatggaat tattgccaat aaggaagaaa aggaattcgg gtatgaataa aattagaatt
     1861 aaaaacaaag agcaaagaca gcataaagag aataataatg aaagtaaatt tctctcactc
     1921 taaatcgaaa atcaacacgc tgattgatgc aatatcaaat gatattgcat cgggtgtgtt
     1981 aaagttggga gataatttac catcgatcaa tgaaacgagc ttaaagttta aaatttctcg
     2041 agacacggtt ttcaaggcgt ataaagaatt aaaaagaaga aatctgattg attcgaaccc
     2101 gatgaaaggt tattttgtaa agggtgaagt aaatcatgtt ttattgctgc tcgatacata
     2161 ttcacctttt aaacaaaatc tatacaatcg ttttgcacag aatttaccca aatcgtggaa
     2221 agttgattta atttttcatc agtacaacaa aaatttattt gatacaatta tcagggaaag
     2281 catcggacgt tacagcatgt atgtggtgat gaatttttcg aatactgaat tttctgaatc
     2341 acttaaatcc atacccgaag gaaaattgct attgcttgat tttggagatt tcgataaaaa
     2401 acagtatccg tatttatgtc agaattttga tcaagcttta tatgattgtt tatctgaaca
     2461 caagccaata atttcaaagt acaagaaaat tgtaatgatt tttccaactg aaaacaatca
     2521 tcctaaaagt tcaatcaaat ttgtgaaaga attttgttca gactcaaata tagcatttga
     2581 attaatcgag gatatttatc ttttaaacaa tatagaagaa aatgtattgt actttataat
     2641 gtcgcatacc gatttggtga gcataatttt agcaagtaaa aataaaaatc tgaaaatagg
     2701 acaggatatt ggtattttgg tgtataacga tgaacctctt ttggaggtaa ttcaggaagg
     2761 aattgccgct atttcgattg attttggatt gctgggcgaa aaagcagctc agtttataaa
     2821 agacaaaatg ttcgttcagg aatttttacc tacaagattt attttcagac catcaatata
     2881 atttttttaa taaattgtca gcacagaata ggacagtaaa attttcaatt taacgatgta
     2941 caatttacaa ttttatgcaa atacaataca ttaatattca aacaaattaa ataaaacatt
     3001 tcagaatgaa agcaacaatt acagggcaag cgcaacagat tgtgcaaacc agaaaagaga
     3061 aatcaggacg tctacagggc gataatccta aaataggaat tcgtccaaca attgatggtc
     3121 ggcagggcgg agtgcgtgaa tcgctcgaag atcagactat gagaatggca ttgaatcttg
     3181 ctaaattttt atcgtcaaac ttaaaataca gcgatggcac tccggttgaa tgtgttattg
     3241 ccgacactac aatcggaaga gtggacgaag ccgccgcttg tgccgaaaaa tttaaaaaag
     3301 aaggtgtttc aattaccata actgtaacgc cctgttggtg ttatggcagc gaaaccatcg
     3361 atatggatcc gcacactatt aaagccgtct ggggtttcaa cggaacagaa cgtccgggag
     3421 ctgtatatct ggcagctgct ttagccggtt ataaccaaaa aggactacct gcatttggta
     3481 tttacggacg tgatgtgcag gatgctggtg acacttcaat tcctgcagat gttcaggaaa
     3541 aattactacg tttttcaaaa gccgccattg ctgcagcttc gatgaaagga aaatcttatt
     3601 tgtctatcgg ttcggtttcg atgggaattg caggttcaat tgtcgatccc aatttctttc
     3661 aagaatatct tggcatgcgc aatgaatatg tggatatgag cgaaattaac cgtcgggttc
     3721 aactgggaat tttcgatcag gaagaatttg caagagccat ggaatggaca tcaaaatact
     3781 gcaaagtaaa tgaaggagaa gataaaaacg atgaggataa aaagaaaaca cgcgagcaat
     3841 tggataaaga atgggaattt gttgtaaaaa tgaccattat tatccgcgat ttgatgattg
     3901 gcaatccgaa actgcgtgaa gcaggatttc tcgaagaagc tttaggacac aatgccattg
     3961 cttcaggttt tcagggtcaa cgtcaatgga cagattttat gcccaacgga gatttctcag
     4021 aagcgatact gaatacttca ttcgactgga atggtattcg cgaacctttt gtagtagcta
     4081 ctgaaaacga ctcactgaac ggcgtagcca tgctttttgg aaaattactt accaatacag
     4141 cacaggtatt tgccgatgtt cgtacatact ggagccctga agctgttgaa cgggtaaccg
     4201 gatataagct ggaaggtatc gcatcgaacg gtttaattca cctgattaat tcaggctcgg
     4261 cggctttaga tggcagcgga cagcaaacca aagatggaaa acctgccatg aaacctttct
     4321 gggaaataac cgatgaagaa gttcagaaaa tgttagatgc tacaacctgg tatcctgcag
     4381 attgtggtta tttccgtggc ggaggatttt cttcaaaatt tgtaacaaaa ggcggaatgc
     4441 cggtaactat ggctcgtgta aatcttgtga aaggtcttgg tcctgtactt caattggctg
     4501 aaggttggac ggtggaaatt cctgccgaag ttcacaaaat cttagatgac cgtaccgata
     4561 aaacatggcc tacaacttgg tttgtacctc gattgaccgg taaaggaaat ttcaaggatg
     4621 tttattcggt aatgaacaac tggggtgcaa atcacggcgc tttcagttac ggacatatcg
     4681 gcgctgattt aattacactg gcttcgatat tacgtattcc tgtttgcatg cacaatgtgg
     4741 aagaagataa aattttccga ccaagcgcat gggctgcatt cgggatggat gccgaaggtt
     4801 cagactatcg ggcatgtgca acttacggag caatgtataa gtaaatgacc ccaaccctaa
     4861 agggagtctc agataattaa tcaatcaatt tctgagaaaa aattaatatc tgatttgaag
     4921 gcatactatt aattattgtt gccttcaaat ctttttatat tagttggata attattctta
     4981 agttagtcaa accttaaccc acatttttta tcactgccgt acgaaagttt ttaaaaatgt
     5041 tgttcttgaa aattcatata tttgt
//
LOCUS       DAOSIB010000110         4925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_15280_length_4925_cov_12.114579, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000110 DAOSIB010000000
VERSION     DAOSIB010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15280_length_4925_cov_12.114579"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..1025
                     /locus_tag="PLD44_08150"
     CDS             45..1025
                     /locus_tag="PLD44_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOF99335.1"
                     /translation="MSYLSFSRAINFIRFIGSYISAFFGINRSTKLSPFFISVEPADF
                     CNLQCPECPVGVKNSLLKERGNFFDLELYRNLIEEMKGTLLHVQFYFQGEPFLNKQLT
                     EMIRYAHDRKLFTSVSTNGQFLTDETAQKIVRSGLDKLIVSVDGTTQEVYEMYRKGGN
                     LQKVFDGINHVIAWKKLFKSLFPMIEMQFLVLKSNEHQMQEMKQIAKNLNVDKLSFKS
                     AQFYDFENGNSQIPANSRYVRYRLNKSGKYELKFSQPNRCSRLLGGAVINAKGEVLPC
                     CYDKQSQYSFGNIRSASFYACWHSDKANSFREQLLLDRHQFDICRNCTGR"
     gene            complement(1106..3955)
                     /locus_tag="PLD44_08155"
     CDS             complement(1106..3955)
                     /locus_tag="PLD44_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOF99336.1"
                     /translation="MNLKTILENRILILDGAMGTMIQRYRLDESAYRGERFSHWNKQL
                     KGNNDLLSLTQPDIISSIHKQYLQAGADIIETNTFNAQRISMEDYGMGEFVREMNLSA
                     ARIARQAADEFTLKNPEKPRFVAGSVGPTNKTASLPSDVNRPASRAVTFDQLVTAYKE
                     QMLALIEGGVDALLIETIFDTLNAKAAIFAAQEAMSELNKKVELMISATIADASGRTL
                     SGQTLRAFMASVAHAPLLSIGLNCSFGAKDLKPYLQEMSAHAPWFVSAYPNAGLPNQF
                     GEYDETPDTMAKQVKEYIDEGLVNIIGGCCGTTPEHIAKYRDLITNAAVRKPKEKTVN
                     LELSGLELLEIISPKIDEWQQKESIEATNSTKEKVSRNNSQEIIDISADPAPKSLFVN
                     IGERCNVAGSRKFLRLINEKKYEEALHIARKQVEDGAQILDINMDDAMLNAAEEMVVF
                     LNHITAEPEIARVPIMIDSSKWEVIEAGLKCIQGKSIVNSISLKEGEEDFLKKAKKVR
                     AYGAAAVVMAFDEKGQADSMERRIEICSRAYRLLTEKAGFPPQDIIFDPNVLAIGTGI
                     EEHNHYAVDFLESVQWIKQRLPYAKVSGGISNLSFSFRGNNTVREAMHSAFLYHAIKA
                     GLDMGIVNAGALPIYEEIEPELLEKVENVIFNRKATATEELIEYAEKIKNTVSEKKSD
                     KKDEWRSLPVAKRLEHALVKGIDEFLDTDLAEALTIYDTALEIIEKPLMDGMNIVGKL
                     FGEGKMFLPQVVKSARTMKKAVTILQPEIEKQKVAGTASSAGKFLIATVKGDVHDIGK
                     NIVSVVLACNNYEVIDLGVMVPSDKIIQTAINENVDLVGLSGLITPSLEEMSNVAAEM
                     EKAGLNVPLLIGGATTSKVHTAVKIAPHYHGPVVYVKDASVNTFIVSQLMNEKTHSHF
                     VSEIAAEYQEIREKQVQKTEVLSLEEARKRKPNFF"
     gene            4161..4907
                     /gene="gpmA"
                     /locus_tag="PLD44_08160"
     CDS             4161..4907
                     /gene="gpmA"
                     /locus_tag="PLD44_08160"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOF99337.1"
                     /translation="MKKLVLVRHGESVWNKENRFTGWTDVDLSEKGMQEAAKAGRLLK
                     EKGFFFDKAYTSYLKRAIKTLNIILDVMDLDWIPVEKSWRLNEKHYGMLQGLNKAETA
                     EKYGEEQVQIWRRSYDVPPAPLDMNDPRSPFLDPRYKNVDKKDLPLTEALKDTVARIL
                     PYWENNIFPSLKENNEILVVAHGNSLRGIVKVLKNISDEDIVGLNLPTGVPYVFEFDD
                     NYQVVNDYFLGDPEEIKKLMEEVANQAKKK"
BASE COUNT         1392 a          962 c          997 g         1574 t
ORIGIN      
        1 gtaattaaaa ttaaaatcaa aaaatgtgcc atttgtttcg ttggatgtct tatttaagct
       61 tttctcgtgc aatcaatttc attcgattta taggttccta tatttcggct ttttttggta
      121 taaaccggtc tactaaactg agcccttttt tcatatcggt tgagccggcc gatttttgta
      181 atttgcaatg tcctgaatgt cctgtgggtg tgaaaaattc cttattgaaa gaaagaggaa
      241 atttttttga tttggagctt tacagaaatt tgatagaaga aatgaaagga actttgctgc
      301 atgtacaatt ttattttcag ggagagcctt ttttgaacaa acaactcact gaaatgattc
      361 gatatgccca tgatagaaaa ttatttactt ccgtgtcgac caacggacaa tttttgactg
      421 acgaaacagc gcaaaaaata gtaaggtcag gattggataa actcatcgtt tcagtcgatg
      481 gcactaccca ggaagtttat gaaatgtaca gaaaaggggg aaatttgcag aaagtattcg
      541 atggtatcaa tcatgttata gcttggaaaa agctgtttaa atctttattt ccaatgattg
      601 aaatgcagtt tttggttcta aagtccaacg aacatcaaat gcaggaaatg aaacaaatag
      661 caaaaaactt gaatgtggac aaacttagtt ttaagtccgc acagttttac gattttgaaa
      721 atggaaattc gcaaattcct gccaacagtc gttatgtgag gtatcgactc aacaagtcag
      781 gaaaatacga acttaaattt tcgcagccca atcgatgcag tcgtttgctg ggtggagcag
      841 taattaatgc aaaaggagag gtgttgccgt gttgctacga caagcagtcg caatactcat
      901 ttggaaatat ccgttctgct tccttttatg cctgttggca ttcggataag gcaaattctt
      961 ttcgggaaca gttgctctta gaccgtcatc agtttgatat ttgcagaaat tgtacgggaa
     1021 gatgaagaat tgcggctgaa aagggttacg agttgtgagt tctaagtcgt tcatgcctga
     1081 aatacgttga ccattttggt tattttcaga aaaaatttgg tttacgtttt cgagcttctt
     1141 caagtgagag cacttcagtt ttctgaactt gcttttcgcg aatttcctga tattcggcag
     1201 ctatttcgga aacaaaatgg ctatgtgttt tttcgttcat cagctgggaa acgatgaaag
     1261 tattgacgga tgcatcttta acatatacca ccggcccatg ataatgaggc gcaattttta
     1321 ctgccgtatg aactttggat gtcgttgctc ccccaataag taagggaacg ttgagtccgg
     1381 ctttttccat ttcggcagca acgttgctca tctcttccag cgaaggagtt atcagtccac
     1441 ttaatccaac caaatcgaca ttttcgttga ttgctgtctg aataatttta tctgagggca
     1501 ccataacacc caagtcgata acttcataat tgttgcaagc caataccaca gatacaatat
     1561 ttttcccaat atcgtgcaca tcacctttta cggttgcaat aaggaatttg cctgccgatg
     1621 aggctgtccc ggcaactttt tgtttttcaa tttcgggctg aagaatggtt acggcttttt
     1681 tcatggttcg agccgacttc accacttggg gtaaaaacat tttaccttct ccgaaaagtt
     1741 ttccaacaat gttcatcccg tccatcaagg gtttttcaat aatttccagt gcggtatcgt
     1801 aaatagttag cgcttctgcc aaatcggtat cgagaaattc atcaattccc tttacaagtg
     1861 catgttcaag gcgtttagca accggaagcg aacgccattc atcttttttg tccgattttt
     1921 tttcggacac cgtattcttt attttttctg catattctat cagttcttcc gtagctgttg
     1981 ctttccggtt gaagataaca ttttccactt tttccaaaag ttcaggctct atttcttcat
     2041 aaattggcag tgctcccgcg ttaacaattc ccatatccaa tccggctttt atggcgtgat
     2101 aaagaaaagc ggaatgcatg gcctcacgaa ccgtattgtt tcctctgaag gaaaaagata
     2161 aattgctgat tcccccgcta acttttgcat aaggcaaacg ttgtttgatc cattgtaccg
     2221 actccagaaa atctacagcg taatgattgt gttcttctat tccggtacca atagccaaaa
     2281 cattgggatc aaaaattata tcctgtggtg gaaatcctgc tttttcggtt agcagacgat
     2341 atgctcggga gcaaatttcg attcttcgct ccatgctgtc ggcttggcct ttttcgtcga
     2401 aagccatcac aacggcagcc gcaccataag cccgcacctt ttttgctttt ttcagaaaat
     2461 cttcttctcc ttcctttaaa ctgatggaat tgacaatgct ttttccctga atacatttca
     2521 aaccggcttc aatgacttcc cattttgagg aatcaatcat tatgggaaca cgtgcaattt
     2581 cgggctcggc agtaatgtga ttcaaaaaaa caaccatttc ttctgctgca ttcagcatgg
     2641 cgtcatccat gttgatgtcc agtatttgcg caccatcttc aacttgtttt cgggcaatgt
     2701 ggagcgcttc ttcgtatttt ttctcgttga ttagccttaa aaacttgcgc gaaccggcca
     2761 cgttgcatcg ttcaccaatg ttcacaaata aagattttgg agccggatcg gctgaaatat
     2821 caataatttc ctgactgtta tttcttgaaa ctttttcttt tgtcgaattt gtggcttcaa
     2881 tgctctcctt ttgttgccat tcgtcaattt taggagaaat gatttccaat agttccaaac
     2941 cgctcagttc caggtttacg gttttttctt tgggttttct gaccgctgca ttggtaatca
     3001 gatcccgata tttggcaata tgttccggag ttgtaccaca gcaacccccg ataatgttga
     3061 ctaaaccttc gtcaatatat tctttcactt gttttgccat tgtgtcgggc gtttcgtcgt
     3121 attcgccaaa ttgattcggg agtccggcat tggggtatgc actgacaaac caaggtgcat
     3181 gagcgctcat ttcctgaagg taaggtttta aatctttggc tccaaaagaa cagtttaatc
     3241 cgatagaaag tagcggagca tgtgcgacgg aagccataaa agcccgaagt gtttgaccgg
     3301 aaagtgtacg tccgctggca tcggcaatgg tagccgaaat cattaattcc acttttttat
     3361 tcaattccga catagcttct tgagccgcga aaatagcggc ttttgcattt aaagtgtcga
     3421 aaatggtttc tatcaacagc gcatctactc caccttctat caaagccagc atttgttcct
     3481 tgtaagctgt taccagttga tcgaaggtta cggcgcgcga agcgggacga tttacgtcgg
     3541 aaggtaagga agccgtttta ttggttgggc ctaccgaacc ggcaacaaat cttggttttt
     3601 ccggattttt gagagtgaat tcatctgctg cctgtctggc tatgcgcgct gcactcaaat
     3661 tcatctccct tacaaattct cccattccat aatcttccat cgaaattcgt tgtgcattga
     3721 aagtgttggt ttcgataata tcggcacctg cttgcaaata ttgcttatgg atggaactaa
     3781 taatatccgg ttgagttaaa gaaagaaggt cgttgtttcc tttcaattgc ttgttccaat
     3841 gagaaaaacg ttcacctctg tatgcggatt cgtcgagcct gtagcgctga atcatggtac
     3901 ccattgctcc atccagaatc aatatgcgat tttcgagaat tgtttttaaa ttcatagtgt
     3961 tgtaaaatag gaaggaaatt ttcaaatata tctgttgttt tatttctgca aaattaatga
     4021 atatgggtca attctcaaaa acagcaactt atccttataa atttccattc tttgattttt
     4081 aaactttcat tattatcgga ttttcaatat ctttgtcatc agtaaaaaaa gtgaatcact
     4141 aacctcttaa taagaagaaa atgaaaaaat tagttttagt aagacatggc gaaagcgttt
     4201 ggaataagga aaatcgcttt actggttgga cagatgttga tttgtctgaa aaaggaatgc
     4261 aagaagcagc caaagcaggc agattgctga aggaaaaggg tttctttttc gataaagcct
     4321 acacttccta tttgaagaga gctatcaaaa ctttgaatat cattcttgat gtaatggatt
     4381 tggattggat tccggtagaa aaaagctggc gtttgaacga aaaacattat ggaatgcttc
     4441 agggtttaaa caaagcagaa acagccgaaa aatacggcga agaacaagtt cagatttgga
     4501 gacgcagcta tgatgttccg cctgcaccgc tggacatgaa tgatcctcgt tcaccgtttt
     4561 tggatccacg ctacaaaaat gtggataaaa aagatttgcc tctgacagaa gctttgaaag
     4621 ataccgtagc tcggattctt ccttattggg aaaacaacat cttcccgagt ttgaaagaaa
     4681 acaacgaaat tctggttgtt gctcacggta acagcttgag aggtattgta aaagttttga
     4741 aaaatatttc cgacgaagat attgtgggtt taaatctacc cacaggtgtg ccctatgttt
     4801 ttgaatttga tgataattat caggttgtaa acgattattt cctgggcgat ccggaagaaa
     4861 tcaaaaaatt gatggaagaa gtggccaatc aggcaaaaaa gaaataagaa gaattcttct
     4921 cttct
//
LOCUS       DAOSIB010000111         4894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_15410_length_4894_cov_9.127919, whole genome shotgun sequence.
ACCESSION   DAOSIB010000111 DAOSIB010000000
VERSION     DAOSIB010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15410_length_4894_cov_9.127919"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1879
                     /gene="typA"
                     /locus_tag="PLD44_08165"
     CDS             62..1879
                     /gene="typA"
                     /locus_tag="PLD44_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOF99338.1"
                     /translation="MQKIRNVAIIAHVDHGKTTLVDKMLLAGHLFRNNEKVGELIMDN
                     NELERERGITILAKNVSINYNGYKINIIDTPGHADFGGEVERVLNMADGVLLLVDAFE
                     GPMPQTRFVLQKAIQMGLIPIVVVNKVDKPNCRPDEVQEAVFDLMYHLDANEEQLNFH
                     TIFGSAKQNWMSDDWKKPTDNILPLLDAIIKYIPAPLYLEGSPQMLITSMDYSPYVGR
                     IAVGRLHRGTLREGMEVSLVSRDGKVTKSRIKELHTFTGLGRTRTEEVKSGDICALVG
                     IEGFEIGDSIADALQPEPLKPIAVDEPTMSMVFTINNSPFYGKEGKFVTSRHIHERLM
                     RELEKDVALRVEKNPDSDIWHVYGRGVLHLSILIETMRREGYELQVGQPQVIFKEING
                     VRCEPVEQLSINLPEEYAGKIIEMVAARKGEMLSMEIVNDRVQLEFQIPSRGIIGLRN
                     NVLTASTGEAVMSHRFLEYQPYKGEIEKRNNGSLIAMESGTAFAYAIDKLQDRGRFFI
                     FPQEEVYAGQVVGESAKEGDIVVNVTKSKKLTNMRASGSDDKIKLTPPVVFSLEEALE
                     YIKEDEYVEVTPTKIRLRKIYLDENERKRMSKKSNVLSE"
     gene            1935..2561
                     /locus_tag="PLD44_08170"
     CDS             1935..2561
                     /locus_tag="PLD44_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOF99339.1"
                     /translation="MKKRIALSGCLLFFLAMNFFSQFSLQGGLNRASEIYVGSSASDA
                     NLPALKNLTGYNIGMMYQTPQKGFGFATGIALSQKGSIVNSDSLTDVYRQFNFLEIPL
                     HLRYCLDFGNMGLYGFGGVYGAYALSGQKISETPETQNNENFTYSDFNCRVDYGYTFG
                     AGIQFFKKIQIQATWNQGWKNLTNYLNGEKEPFSTKNKIFAVNLSYLF"
     gene            2706..3131
                     /gene="ssb"
                     /locus_tag="PLD44_08175"
     CDS             2706..3131
                     /gene="ssb"
                     /locus_tag="PLD44_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005851334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOF99340.1"
                     /translation="MSVNKVILVGNVGKDPEVRYLEKNVAVARFPLATTERGYTMQNG
                     TQVPDRTEWHNIVAWRGLAEIVEKYVKKGSQLYIEGKIQSRSWEKDGIKRYTTEIYAE
                     NIQLLGKRSDTSEEPAAASTPPAETFQEGADIVEDDLPF"
     gene            complement(3333..4520)
                     /locus_tag="PLD44_08180"
     CDS             complement(3333..4520)
                     /locus_tag="PLD44_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOF99341.1"
                     /translation="MSLLSTVMGQYDEPQKVKSLGIYPYFRPIQSEQDTVVTINGKPV
                     LMFGSNSYLGLTNHPKLKEAAIRAIEKYGTGCAGSRFLNGTLDIHIELENRLAKFVHK
                     DAALVYSTGFSVNSGVIPCLTGRNDYLIFDERDHASIIEGKRLSFSKQFKFRHNDMDS
                     LEDVLKRCDYDKIKVIVVDGVFSMEGDIAKLPEIVELSKKYNASIYVDEAHTLGVFGK
                     TGAGICEHYGHTNDVDLIMGTFSKSLGTIGGFIASDNNIINYLKHNSRTLIFSASITP
                     ASAGCVLAALDVLEEETWRKEALWKNTARAKDGFMKAGFEIGHTESPIIPLYVRDNLK
                     TFQLTSLLMEEGVFVNPVVSPAVPSEDTLIRYSLMATHTFEQIDESIEKITKVARKLG
                     IIE"
BASE COUNT         1552 a          953 c          971 g         1418 t
ORIGIN      
        1 ggcaataaaa attcaatttt tttggctatc tttgcaggct aaaaaattag ctctttaact
       61 tatgcaaaaa attagaaatg tagccatcat tgcccatgtc gaccacggga aaactacatt
      121 ggtagacaag atgttactgg caggacacct tttccgcaac aacgaaaaag ttggtgaact
      181 cattatggac aataatgaac tcgaacgcga aagaggaatc accatattgg ctaaaaatgt
      241 ttccatcaat tacaacggat ataaaatcaa cattatagac acaccgggac acgccgattt
      301 cggaggagaa gtagaaaggg ttttaaatat ggctgatgga gtgttacttt tggtagatgc
      361 ttttgaaggt cccatgccac aaacccgttt tgttctccaa aaagccattc aaatgggact
      421 gattcccatt gtggtcgtca ataaggtcga caagcccaat tgccgtcccg atgaagttca
      481 ggaagccgtc ttcgacttaa tgtatcattt ggatgccaat gaagaacagt tgaattttca
      541 cactattttt ggatcagcca aacagaactg gatgtcggac gattggaaaa aaccaaccga
      601 caatatttta cccctgctgg atgccattat caagtatatt cctgctccgc tctatttgga
      661 aggctctccc caaatgctta tcacttcgat ggattactct ccgtatgtgg gaagaattgc
      721 cgttggcaga cttcatcgtg gaacacttcg cgaaggaatg gaagtaagcc tggtaagtcg
      781 tgatggcaag gttacaaaat cccgcattaa ggaattgcat acttttaccg gacttggaag
      841 aacgcgcacg gaagaagtga aatcgggtga tatttgtgcg ctggtgggaa tagagggttt
      901 tgaaattggc gattccattg ccgacgcact ccagccggaa ccgttgaaac ccattgcggt
      961 ggatgagcct accatgagta tggttttcac catcaataat tcgccttttt acggaaagga
     1021 aggaaaattc gttacttcac gacacattca tgaacgattg atgcgagaac tcgaaaaaga
     1081 cgtcgcttta cgcgtggaga aaaatccaga ctcggatatt tggcacgtct atggaagagg
     1141 tgttttacac ctttccattc tgatagaaac catgcgcaga gaaggttacg aacttcaggt
     1201 aggacagcca caggtaattt tcaaagaaat aaacggcgtt cgttgcgaac cggtcgaaca
     1261 gttgagcatc aatctccctg aagaatatgc cggaaaaatc attgagatgg ttgcagcccg
     1321 caaaggcgaa atgctgagca tggaaatagt aaacgaccgt gtacagctgg agtttcaaat
     1381 tccttcacga ggtattatcg gattaaggaa taatgtacta acagcttcaa caggagaagc
     1441 cgttatgtcg caccgatttt tggaatacca accttacaag ggtgaaatcg aaaaaagaaa
     1501 caatggatcg cttattgcaa tggaaagtgg cacggcattt gcttatgcca tcgacaaatt
     1561 acaggacaga ggcagatttt tcatttttcc ccaggaggaa gtttatgccg gtcaggttgt
     1621 gggtgaaagc gctaaggaag gcgacattgt ggtgaatgtt accaagtcga aaaaattaac
     1681 caacatgcgg gcatcaggtt ctgatgacaa aattaaactt acaccaccgg tagttttcag
     1741 tctcgaagaa gctttggaat atatcaaaga agacgaatat gtggaagtta ctccaaccaa
     1801 aattcgctta aggaaaatct atcttgatga aaatgaacgt aaacgcatgt cgaaaaagtc
     1861 gaatgtcctc agtgaataag aatttatttc ggaacaaaac gattccatta aaaaagagcg
     1921 gtacacaaga atttatgaaa aaaagaatag ccttgtcggg atgtttgtta tttttccttg
     1981 caatgaattt tttttcgcaa ttctccctac aaggcggcct caacagagct tcagaaattt
     2041 acgtcggcag tagtgcttct gatgccaacc tgccggcttt aaaaaacctg acgggataca
     2101 acattgggat gatgtaccaa actccacaaa aaggctttgg ttttgccaca ggtattgccc
     2161 tttctcagaa aggcagtatt gtaaattccg acagcttgac agatgtttac cgccaattca
     2221 attttctgga aatccctctg catttgcgct attgtctgga ttttggaaat atggggctgt
     2281 atggatttgg aggagtttac ggcgcctatg ccttgagtgg acaaaaaatt tctgaaacac
     2341 ctgaaaccca aaacaacgaa aattttactt attccgattt taattgtcga gtcgactatg
     2401 gctacacatt tggagccgga attcaattct tcaaaaaaat tcagattcag gctacttgga
     2461 atcagggatg gaaaaatcta accaattatt tgaatgggga gaaggaacct ttttctacta
     2521 aaaacaaaat atttgccgtc aatttgtcat atttgtttta aacaaccatt ctattttaca
     2581 atcatcgacg acaaaaatca agctcatttt tttcaaaatt tttgaaaaaa atttgatacg
     2641 tataaaaaaa aatgtatttt tacgggcagt tcaactcaat tcaaaccatt ttaatcattg
     2701 aaattatgtc tgttaataaa gttattttag tagggaatgt aggaaaagat cccgaagtgc
     2761 gttacttgga aaaaaatgtt gcagtagccc gttttccttt ggctaccact gaacggggtt
     2821 ataccatgca aaacggaacg caagttcccg atcgaactga atggcacaat attgtagctt
     2881 ggagggggct ggcagaaatt gtagaaaaat acgtaaaaaa aggaagtcag ctttacatcg
     2941 agggaaaaat tcaaagtcgc tcatgggaga aagatggaat taaacgttac actacggaaa
     3001 tttatgctga aaatatccaa ctgttaggaa aacgttcgga cacatctgaa gaacctgctg
     3061 cagcttctac tccccctgca gaaacctttc aggagggtgc tgatatagta gaagacgatt
     3121 tgcctttctg aaaaaattct tattttctcc aaagcacatg acgctattgt tgtgtgcttt
     3181 ttttgttcta cctgttttta tcctaccata ttttaaaact tgcacaaaat atataaatgg
     3241 ttatcgtcag tctatttctt taaaagcaaa acagaatgta tcaataattt gacacattct
     3301 gttgacaatt gaatgaaaaa actttcgtgt aattattcta taattcccaa cttgcgggca
     3361 acttttgtaa ttttttctat tgattcgtca atctgttcaa acgtatgggt agccatcaac
     3421 gaatatctga tcagtgtatc ttcggaggga acagccggcg aaacaacagg attaacaaaa
     3481 actccttctt ccatcagcaa actggtaagt tgaaaagtct taagattatc ccgcacatat
     3541 aaaggaatga tgggtgattc agtgtgccca atttcaaaac cggccttcat aaatccatct
     3601 ttcgcgcggg ctgtgttttt ccacaacgct tcttttctcc acgtttcttc ttccaataca
     3661 tccaaagctg ccagcacgca acctgcagaa gcaggtgtaa tggaagcgct gaatatcaat
     3721 gttctcgaat tgtgtttcaa ataattgata atattgttgt cggaagcgat aaaaccgcca
     3781 atggttccca acgatttgct gaaagttccc ataatcagat caacatcatt ggtatgcccg
     3841 taatgctcgc aaattcctgc tcctgttttt ccgaaaacgc ccagagtatg agcttcatca
     3901 acataaatgg aagcattgta ttttttggat aattccacaa tctcaggcaa tttggcaatg
     3961 tcgccttcca tactgaaaac gccatccact acaatcacct ttattttgtc ataatcgcaa
     4021 cgttttagca catcttccag cgaatccatg tcgttgtgac ggaatttaaa ctgttttgaa
     4081 aacgaaagac gctttccctc aataatagaa gcgtgatctc tttcgtcaaa aatcagataa
     4141 tcgtttcgtc ccgtcaaaca tggaattaca cccgaattta ccgaaaaacc agtggaatat
     4201 accaaagcag catccttatg aacaaatttt gccaaacggt tttccaattc gatatgaata
     4261 tcaagtgttc cattcagaaa ccgcgaacct gcacaacctg tgccgtattt ttctattgct
     4321 ctgatagcgg cttctttcag tttcggatga ttggtaagac ccagataact attggatcca
     4381 aacattaata caggtttccc gtttatggta acaactgtat cctgttccga ctgaatagga
     4441 cgaaaataag gataaatgcc caatgatttt actttctgag gttcatcata ctgccccatt
     4501 actgtactta acaaactcat aagttatagt ttatattttt attaaaaacg gcacaaagat
     4561 acatcttttt gattaataat taatgctttt taaaaacaga aaattttaga ttcatatttc
     4621 atcagcatat aatttatttt tttaaggtct gatggaaccc acatgtctgt atattacatt
     4681 cattcgtgtt tgtgtatgca atacttgtgg gtttcatagt ttagtacatg acataaattg
     4741 agacatttgt tttatatatc tgtaaaacaa aacatataag ccaagcactt gggttgacct
     4801 tgtccgatga gccgaaaaac aagcgagaaa agcctttata aatcgtcctt gtttgagttc
     4861 cgacgtattt cgtcggaatg agtttgggcg attt
//
LOCUS       DAOSIB010000112         4823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_15670_length_4823_cov_11.211619, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000112 DAOSIB010000000
VERSION     DAOSIB010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15670_length_4823_cov_11.211619"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..673)
                     /locus_tag="PLD44_08185"
     CDS             complement(26..673)
                     /locus_tag="PLD44_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOF99342.1"
                     /translation="MRCGNCGCLITAETQKGHNYYRCTKKKQKCEEKYLREENLVEQM
                     KSWIQKISLPDNWAENMFAEIDKEKDQAKKDGKSFVQNLQKQKLEVEQKIEKLLDPYI
                     ASGTLTLEEYPAKKQKLLDEKLEIEQKLRNFEQSGSNWLEPMREMIFEAKQAKILLSQ
                     GDNQQILTFLKNVDSNFILKDKKLNFCFKSGLRALTEGEPTKTFSNWRRRRDSNP"
     gene            complement(737..1402)
                     /locus_tag="PLD44_08190"
                     /pseudo
     CDS             complement(737..1402)
                     /locus_tag="PLD44_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(1419..3428)
                     /locus_tag="PLD44_08195"
     CDS             complement(1419..3428)
                     /locus_tag="PLD44_08195"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOF99343.1"
                     /translation="MQALNLFNLAEKTKVRKMIKIADLKPINNLKSIFREIRDYFAGN
                     VTGITRDEKIAQNIMRLLFCKIYDEKNSREITEFSNRPDDNHETFKKRINNLFEKVKK
                     NYPDIFEQSEKIEIKPEELSFIVSKLEDFSIINADRDVIGDAFEELIGTSFRGGEGQF
                     FTPRNVVQMMIDVLSPREGERIIDPACGSGGFLAYTLRHFISSNKKNFYLTGIDKDAF
                     LSKIAKIYLSLLGNDEFQIFCENSLEVPEKWGSDTKKKIELGTFDVILTNPPFGAKIP
                     VIGKELLSQYQLGHKWENGNKWIQTNKLLDKQAPQVLFIERCLQLLRDGGRLGIILPE
                     GIFGNPSDRYIWEYISSVASITGVVSLSQETFQPSTHTKTSVLFLKKTTERPKSIFMA
                     IANSVGHNKNGKPVYKIDKYSNGKMLDDDLPIIAKKFNSYKNNNKSLSDHLGFNISYD
                     ELNDHIFIPEYYNPEIRKELETLKKGGQYLLLSVGDLIDKRILQVRRGNEIGSQFYGT
                     GEIPFVRTSDIVNWEIKFDPVKAVSEDVYNQYKKAQDIQENDILFVNDGTFLIGRTAM
                     VTKLDLKIIIQSHIRKIRVLDNEFINPFYLFYLLNSKIVRKQIDSKTFVQATISTIGN
                     RLEEIILPINKDQNEVKKIIREIKDIIEKKADLRDKTVRIVEASI"
     gene            complement(3434..4441)
                     /locus_tag="PLD44_08200"
     CDS             complement(3434..4441)
                     /locus_tag="PLD44_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99344.1"
                     /translation="MATGRTISASFLTDLDHKIIELCDSISKTIYGSIPDEEAKKEFK
                     KEYGKISYTRDGGLGLGGGKIQRDAICTRGRQGKAPFSNRNLRWHPLIVAENKPDFAR
                     KIERIEIGGQDDGQILIFVIKDAKGREFKFPSDKVHEMVERFVALPEHWYPHINILKH
                     WSDTLWTQNSCVIPALEACNWWDAVETYAVLGLALAVDLYGADFDSLYKKIRQILEKQ
                     GIDKRISLPTSIFPTKKEDIVKCPVCLLNISKDLEGFRTSERGKTWQPAWRSSKKEEG
                     DDSSIQIMHVNPLIENHIKHNASNVRYGHRWCNVSMTDHSLDETLDFMEFVVRAHKRC
                     K"
     gene            complement(4446..4652)
                     /locus_tag="PLD44_08205"
     CDS             complement(4446..4652)
                     /locus_tag="PLD44_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOF99345.1"
                     /translation="MATEINIGENVKKHRTKQGLSQEDFAKESGVKYTTLTKIESNVI
                     KKPSVLIMAKLAKALGVNIEDLIK"
BASE COUNT         1330 a          964 c          739 g         1790 t
ORIGIN      
        1 cgaaaagcgc tatgcctata tggcatcaag ggttcgaatc ccttctcctc cgccaattgg
       61 aaaatgtctt tgttggctcg ccctctgtga gggctcgcaa gccgcttttg aagcagaaat
      121 ttaatttttt gtctttcaaa ataaagttcg agtcgacatt ttttagaaat gttaggattt
      181 gctgattgtc gccctgcgac aagagaattt tagcttgttt cgcctcaaaa atcatttctc
      241 tcatcggttc gagccaatta ctccctgatt gttcaaaatt tcttaatttt tgctcaatct
      301 ctaatttctc gtcgagtagt ttttgtttct tggctgggta ttcctcaagc gtcaaagtcc
      361 cactggcaat gtaagggtct aacaattttt caattttctg ctcaacttct aatttttgct
      421 tttggagatt ttgaacaaaa ctttttccgt cttttttggc ttggtctttt tctttgtcta
      481 tctcggcaaa catattttcc gcccagttat caggcaaaga aattttttga atccaggatt
      541 tcatctgttc aacaagattt tcttctctta gatatttttc ctcgcacttt tgctttttct
      601 tggtgcaacg ataataattg tgtccctttt gggtttcagc agtaatgagg caaccacaat
      661 ttccgcaacg cattaaacct gaaaaagcaa actcatgctt tctttttctc tttgtcttgc
      721 cccgattatt cattacctgt tgaacagaat caaaaagttt ctttgaaata attggctcat
      781 ggcttccctg ataaacttcg ttgttaaacc taaatactcc gtagtaaaaa tgatttttga
      841 gcattcgctg gacacaggaa gcagaaagcg gtttgttgcg gtagcttttt attccgctat
      901 ctgccaaaaa ctgccttatg gcttttgggg tatagtcgcc ggtggcgcaa agttcaaacg
      961 cttttctaat aaatggcgct ttgtctttgt ctaaatctat ttcttttgtt cggtggttgt
     1021 ttagatagcc aagcggtgca aagccgggcc aaattccttt tcgcagtttt tggcggtggc
     1081 cttgcttgat attctcgctt gagttgtcca catagtattt gctttgcccg aaagcgatcg
     1141 aaagcataaa ttttccctgc ggcgtatttt caaaccaaaa agtcgggaat ttcaaatcct
     1201 taatttttcc tgggtccagc atataaatta ttttgcccct gtctattgaa tttcttgcca
     1261 atctgtccgg atgccaagcc aaaatttcac tggcttcgcc agcgcaaatc cggtctaaca
     1321 tttctccgaa aactttttgg ccaggttctt tggcggtttg cgcctcgcat agcgaggcgg
     1381 cgatttcaag tttttctttg gcggcaaatt cttttttatc aaatactcgc ctctacaatc
     1441 ctaactgttt tatctcttaa atcagctttt ttctcaataa tatctttaat ttctcttata
     1501 atttttttaa cttcgttctg atccttattt attggtaata taatttcttc taatctattg
     1561 ccaatagttg aaatggttgc ttgtacaaat gttttagaat caatttgttt tctaacgatt
     1621 ttgctattca aaagataaaa caaataaaat ggattgataa actcattatc taacacccga
     1681 attttcctta tatgactttg aatgattatt ttcaaatcca gttttgtgac catggcagtt
     1741 cttccgatca gaaatgttcc atcattcaca aataaaatat cgttttcttg aatatcctgt
     1801 gcttttttat attgattata aacatcttca gacacagcct ttactggatc aaattttatt
     1861 tcccaattaa caatatcgct tgttcttaca aatggtattt ccccagtacc ataaaattga
     1921 gaacctatct catttcctct cctgacttgt aaaatccttt tgtctattaa atcgccaacg
     1981 gatagcaata aatattgtcc acccttttta agcgtctcta actctttgcg aatttcagga
     2041 ttgtagtatt cgggaataaa aatatggtca tttaattcgt cgtaagaaat attgaatcct
     2101 aaatgatcag atagggattt attattgttt ttgtatgaat taaacttttt agcaattata
     2161 ggaagatcgt catctaacat ctttccatta ctatatttat ctattttgta tactggtttt
     2221 ccatttttat tatgacctac gctatttgcg atagccataa aaattgattt tggcctttca
     2281 gttgtctttt ttaagaacaa aacacttgtt tttgtatgcg tgcttggttg aaaagtttct
     2341 tgagataaac ttacaacacc agtaattgac gcgacacttg aaatatactc ccaaatatac
     2401 ctatcagatg gattgccaaa aattccttca ggcaaaataa ttccaagcct tccgccatct
     2461 cgaagcaact gtaagcacct ctcaatgaat aagacttgcg gtgcttgttt atctagtaat
     2521 ttatttgttt gtatccattt attgccgttt tcccatttat gaccaagttg atattgactt
     2581 agtagttctt ttccgatgac tggtattttt gctccgaatg gtggatttgt gagtattaca
     2641 tcaaaagtac caagttcaat cttcttcttg gtatcacttc cccatttttc aggcacttct
     2701 aaactatttt cacaaaagat ctgaaactca tcattaccaa gtaatgagag atatattttt
     2761 gcgatttttg acaaaaaagc atctttatca ataccagtga gataaaaatt ttttttatta
     2821 ctcgaaataa aatgtcttag cgtataagca agaaaaccac cactaccaca agctggatca
     2881 ataattcgtt ctccttctcg aggagacaag acatcaatca tcatttgcac aacatttctc
     2941 ggcgtaaaaa attgtccctc gccaccccta aaagaagtac ctattaattc ttcaaaagca
     3001 tcaccaataa catccctgtc agcatttatg atcgaaaaat cttctaattt tgagactata
     3061 aatgaaagtt cctccggctt tatttctatt ttttcactct gttcaaaaat gtctggataa
     3121 ttttttttta ccttttcaaa taaattattt attctttttt taaaagtttc gtgattatcg
     3181 tctggtcggt tagaaaactc ggttatttct cggctatttt tttcgtcata aatcttacaa
     3241 aacaaaagtc gcattatatt ttgcgcgatt ttttcatctc ttgtaatacc agtaacatta
     3301 ccagcaaaat aatcccggat ttcccgaaat atcgacttaa ggttgtttat tggctttaaa
     3361 tcggcaattt ttatcatttt tctaactttt gttttttcag ccagattaaa taaatttagt
     3421 gcttgcataa gatttattta caccttttat gtgcgcgtac tacaaattcc ataaagtcta
     3481 aagtttcatc taacgagtga tcggtcatag aaacattaca ccatctgtgg ccatatctaa
     3541 cattgcttgc gttatgttta atatgatttt caataagagg atttacgtgc attatttgaa
     3601 tacttgaatc gtcgccctct tccttcttag aagaacgcca ggctggttgc catgtcttac
     3661 ctctttcaga tgttctaaat ccttctaaat cctttgagat gtttagaaga caaacggggc
     3721 acttaacaat atcttctttc ttcgttggga aaatacttgt tggtaatgaa atccttttat
     3781 caataccttg tttttctaat atctgcctaa tttttttata aagagaatca aaatcagctc
     3841 cgtacaaatc aacagctaaa gcaagcccca gaacagcata ggtttcaaca gcgtcccacc
     3901 agttacacgc ctcaagagca ggaattacac aagagttttg tgtccataat gtatcagacc
     3961 aatgttttaa aatatttata tgtgggtacc aatgttctgg aagtgcaaca aatctttcca
     4021 ccatttcatg cactttatct gaaggaaatt taaactctct accttttgcg tccttaatga
     4081 caaaaattaa aatttgtcca tcatcttgcc cgccaatttc tattctttct atttttcttg
     4141 caaaatcagg tttattttcc gcgacaatta aagggtgcca ccttaaattt ctatttgaaa
     4201 atggggcttt accctggcgt ccccttgtgc atatagcgtc tctttgtatt tttccaccgc
     4261 caagccccaa cccgccatct ctagtgtagc tgattttacc atattctttt ttgaattcct
     4321 ttttagcttc ctcgtctgga atagagccat aaatcgtctt tgaaattgaa tcgcataatt
     4381 caataatttt atgatctaaa tctgtaagaa aactcgcgct tattgttctt cctgtagcca
     4441 tatgtttatt ttattaaatc ttcaatatta acgcccaatg ctttagctaa ttttgccata
     4501 ataagcacgg acggcttttt aattacattg ctttcaattt ttgtcagggt agtgtatttt
     4561 acgccggatt ctttggcaaa atcctcttgc gataaacctt gctttgttcg gtgttttttt
     4621 acattttcac ctatgttgat ttctgttgcc atatttttat ttgatagtat gtaaatgata
     4681 tgctattctt tcaatataat gataccaaat aaaattaaat caatcaaaca aaaaatgaag
     4741 aaaagcccgc ttctggccaa aataaagttc gagccgacat tttttcaggt tctttggcgg
     4801 tttgcctttt cgcttccgct tca
//
LOCUS       DAOSIB010000113         4811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_15721_length_4811_cov_12.579899, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000113 DAOSIB010000000
VERSION     DAOSIB010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15721_length_4811_cov_12.579899"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..1799
                     /locus_tag="PLD44_08210"
     CDS             18..1799
                     /locus_tag="PLD44_08210"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOF99346.1"
                     /translation="MMQNIKERISALRNEMKINDIAACIIPSSDPHASEYIAEHWKER
                     EWISGFNGSAGTVVITLTRAALWTDSRYFLQAENQLQGSGMELMKEGLPETLSWTDWL
                     AEELKNNEKVGLNAQMFSYNAFTHLKNELNKKGISLIAVDLIGKIWKDRPALPSQPFY
                     VYDIRYAGETASNKLASLRAQMKKNQADIFVLSALDEIAWLFNIRGSDVDFNPVVIAY
                     AIIKIDKATLFVDSKKVTEKTAAFLQEEGITIEPYNDIYSQLKKITEGKRVLIDGSKL
                     NAALYEAIPQHCSIINTMSPVLKMKSVKNKIELEGTRWAMVKDGVALVKFFKWLEENI
                     LSSEISEISAAEKLFECRQQQENFVSESFDTICGYAGHGAIVHYHAEPETDIVLKPDN
                     LLLVDSGGQYLDGTTDITRTIALGIPTQQQKIDYTLVLKGHIALAKAKFPTNTRGSQL
                     DILAHKWLWEKGLNYGHGTGHGVGHFLCVHEGPQNIRMDENPAVLLPGMIISDEPGLY
                     RTNAYGIRIENLVLVVEDEKTEFGDFLCFETLTLFPYDRSLIELSMLTEDEINWINSY
                     QKKVLEKLSLYLNEEEKIWLQKKCASL"
     gene            complement(1911..4349)
                     /locus_tag="PLD44_08215"
     CDS             complement(1911..4349)
                     /locus_tag="PLD44_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOF99347.1"
                     /translation="MRTKLLLSLGLLGTMSFLYGQQYRPGKQIDHDNTAFVRKVELKK
                     AGEVMMKVDQISVTDTTSELSASLYSPDFTLFKNFHAPVPADGAQFFFGHGGVTVAEN
                     LVNNQPRYYFLYQTEGYSEDESGFRFFQDIHIIDENQQQVGKVPTSWMGYQLYMQDGK
                     NMLISDYQDWDTIFWSNPETGWSGWETFQVHTSAVTDVATGQTIFTFPRGTTFSNFNG
                     MQSGIYYATNEQNLFFNIDGEERILTTNNNFEKYYLGHSSGNHENALDMWIYNKDFSL
                     YKKISNVGYDIPIENLYMANTYQPLFGKHAAYFYHTVTYQTDGGLNYNSKTYLVNLNG
                     ELVKTIDGNFSIMSVVYLPQQDKDVALSMNWTNGAIEVLSIPDFEVIGEFNDLIKDKN
                     GNVKFVKYTDDALIIMNEDFSTYKSIPLPDKNTWSFQMVDYYSTCADEKIEFVFTGKE
                     TETGTGKGLLVINEDNETIIDMPDFSALHSSYYADNLPIILRKSTDKYGRPVSDEFIS
                     YYRTGKLPTQIKLGGNPLPGADMQILKQTDNELIALDKITMNADGKGEIELGEGVYYL
                     KALQTQNGLNTYYPNVLLWENATAIPYNTDPLEEGSMYCIEMQSIPVPVNPTAQGVIV
                     GKIVISQPEAVQQLMRIKLVTDNGSVPIFDVYLQNTADNSIIDHTQTDENGNYRFENV
                     PAGSYRVLVNVDGKPMESVNTVTLTTDADIVENVNYEITETGVVAKMPSGLKYTSTTL
                     IIYPNPAKDFIYLPQSAQNAKVQVLDVQGRILLNIDSYKGEKLDVSRLSNGWYMIQIN
                     NGNRIKTGKFIKIN"
     gene            4680..4761
                     /locus_tag="PLD44_08220"
     tRNA            4680..4761
                     /locus_tag="PLD44_08220"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4714..4716,aa:Leu,seq:tag)
BASE COUNT         1442 a          888 c          933 g         1548 t
ORIGIN      
        1 aaaaattaaa atgaatactg atgcaaaata taaaagaaag aatctcggca ttgcgcaacg
       61 agatgaaaat aaatgatata gctgcctgta ttattccgag tagcgatcct catgccagcg
      121 aatatatcgc cgagcattgg aaagagcgcg aatggatttc gggatttaac ggttcggctg
      181 gtacagtggt aattactttg acaagagctg cgttgtggac cgattcgcga tattttttac
      241 aggcagaaaa tcagttgcaa ggctcaggta tggagctgat gaaggaagga cttccggaaa
      301 ctttgagctg gactgactgg ttggcagaag aactgaaaaa taatgaaaaa gtgggactaa
      361 atgcccaaat gttttcttac aatgctttta cccatctgaa aaacgaattg aacaagaagg
      421 gaatttcact gattgctgtc gatttgattg gtaaaatttg gaaagaccga cctgctttgc
      481 cttctcagcc tttttatgtt tacgatattc gttatgcggg tgaaacggca agcaataaac
      541 tggcctccct gcgcgcacag atgaaaaaaa atcaggcaga tatttttgtg ctttcggctt
      601 tggatgaaat tgcttggttg ttcaacatta gagggagcga tgtggatttt aatccggtgg
      661 taatagctta tgcaataata aaaatcgata aggctaccct ttttgtcgat tcgaaaaaag
      721 ttacggaaaa aaccgctgcc tttcttcaag aggagggcat aaccatagag ccgtacaacg
      781 acatctattc ccaattgaaa aaaattactg aaggaaaacg agttttgatt gatggctcca
      841 agttgaatgc tgcgttgtat gaagctattc cgcaacattg cagcattatc aatactatgt
      901 cgccggtttt gaaaatgaag agtgtaaaaa ataaaataga attggaaggt actcgatggg
      961 caatggtaaa agatggtgtt gcgctggtaa aatttttcaa gtggctggaa gaaaatattt
     1021 tatcttcaga aatcagcgaa atttccgctg cagaaaagtt gttcgaatgc aggcaacaac
     1081 aggaaaattt tgtttcggaa agtttcgata ccatttgcgg ttatgctggt catggggcta
     1141 ttgttcatta tcatgccgaa cctgaaaccg atatcgtttt aaagcctgat aatctcttac
     1201 ttgtcgattc gggtggtcaa tatttggatg gaactaccga tattacccga accatagcgt
     1261 tgggaattcc cactcagcaa caaaaaatag attatacgct tgtattgaaa ggtcatattg
     1321 cacttgcaaa agctaaattc cctaccaaca cgcgtggatc ccaactcgac attcttgcgc
     1381 acaaatggct ctgggaaaag ggtttgaatt acgggcacgg aacaggacat ggcgtaggac
     1441 attttctttg tgttcacgaa gggccacaaa atattcggat ggatgaaaat cctgccgtgc
     1501 ttttgccggg aatgattatt tccgacgagc cgggtcttta tcgaaccaat gcttatggca
     1561 tacgcattga aaatctcgtg ctggtcgtgg aagacgaaaa aactgagttt ggtgattttc
     1621 tgtgtttcga aacactcaca ctttttcctt acgatcggtc gctgattgaa ctttccatgc
     1681 tcactgagga cgaaataaat tggataaatt cttatcagaa aaaagttttg gaaaagcttt
     1741 ctctttattt gaatgaagaa gagaagattt ggctgcagaa aaaatgtgct tctttgtaat
     1801 ttctttaaaa aaagagtatt gtttaatctg tttgcaaatt gaattttata aaaatatctg
     1861 acttatatta ctgtaagcca gatatttttt cagtatagtt gttttgagcg ctagttgatt
     1921 ttaatgaatt tacctgtttt tattctgttc ccattattta tttgaatcat ataccaacca
     1981 tttgaaagtc tcgacacatc aagcttttct cccttataac tgtcgatgtt caataaaatt
     2041 cttccttgca catccaaaac ttgaactttg gcattttgtg ccgactgtgg aagatatatg
     2101 aaatctttgg ccggattcgg atagattatc agcgtcgttg aagtatattt caatccggaa
     2161 ggcattttgg caacaacacc ggtttcggta atttcataat ttacattttc aacaatgtcg
     2221 gcatcggttg tcaatgttac tgtattgacg gattccattg gtttcccgtc aacattcacc
     2281 aaaactcgat acgaacctgc cggcacattt tcgaaacgat aatttccatt ttcatcggtt
     2341 tgtgtatggt caataatgga attgtcggct gtgttttgca aataaacatc gaaaattgga
     2401 acagaaccat tgtcggtaac cagttttatt cgcatcagct gctggactgc ttccggttga
     2461 ctgataacaa ttttaccaac aataactcct tgtgctgttg gatttacagg aaccggaata
     2521 gattgcatct caatgcaata catacttcct tcttcaagcg gatcggtatt gtaaggtatg
     2581 gcagttgcat tttcccataa cagcacgttg ggatagtaag tattcaatcc gttttgggtt
     2641 tgcagtgcct tcaagtaata aacaccttca cccaattcaa tttctccttt tccgtcagca
     2701 ttcatcgtaa ttttatcgag tgcaataagc tcattatcgg tttgtttcag tatttgcata
     2761 tcggctcctg gcaatggatt gccaccaagt ttgatttggg ttggcaactt gcctgtgcga
     2821 tagtaggaga taaattcatc cgataccgga cgaccatatt tatcagttga ttttcttaaa
     2881 ataataggca agttatcagc ataataagaa gaatggagtg cgctaaaatc tggcatatca
     2941 ataatggttt cgttatcttc attgataacc agcaatcctt ttccagttcc agtttcagtt
     3001 tcttttccgg taaatacaaa ttctattttt tcgtctgcac acgttgaata ataatctacc
     3061 atttggaaac tccatgtgtt tttgtcaggc aagggaatag atttgtaagt actaaaatct
     3121 tcgttcatga ttatcaatgc atcgtcggta tattttacaa attttacatt tccattcttg
     3181 tctttaatca aatcgttaaa ttctccaatt acttcaaagt cgggaatact taaaacttct
     3241 attgccccat ttgtccaatt catgctcaat gccacgtctt tgtcctgttg tggtaaatat
     3301 accacgctca tgatactgaa atttccgtcg atagttttaa ccaactcacc attcagattt
     3361 accaggtatg ttttactgtt gtaatttaag cctccatctg tttgataggt aaccgtgtga
     3421 tagaagtaag cagcatgttt tccgaaaagt ggttgatagg tattggccat atataagttt
     3481 tcgatgggaa tatcatatcc cacatttgaa attttcttat acaatgaaaa atctttgttg
     3541 taaatccaca tgtcaagggc attttcatga tttcctgagg aatgtcccaa ataatatttt
     3601 tcaaaattat tgttggtagt gagaattctt tcttctccat caatattgaa aaacagattt
     3661 tgctcatttg ttgcatagta tatccccgat tgcattccgt tgaagttcga gaaagtagtg
     3721 cctcgtggga aagtaaaaat ggtttgccct gtagctacat cagtaactgc agaagtatgc
     3781 acctgaaaag tctcccaacc cgaccatccg gtttcaggat tactccaaaa tatcgtatcc
     3841 cagtcctgat agtcggatat cagcatgttt ttgccatctt gcatatatag ttgataaccc
     3901 atccacgaag ttggcacttt ccccacttgc tgctgatttt cgtctatgat atgaatgtct
     3961 tgaaaaaatc taaaaccgga ctcgtcttca ctatatcctt ctgtttgata aagaaaataa
     4021 taacgaggct gattgtttac caagttttca gccacagtta cgcctccgtg tccgaagaaa
     4081 aattgtgctc cgtcagccgg aacgggagca tgaaaatttt taaaaagcgt aaaatcgggt
     4141 gaatacaatg aagctgacaa ttcagaagtt gtatcggtaa cagaaatttg atcgactttc
     4201 atcatgactt ctcccgcttt ttttaattcg acttttcgta caaaagcagt gttatcatgg
     4261 tctatttgtt ttccgggtct gtattgctgc ccatacaaaa aggacattgt tcccaacaaa
     4321 cccaatgata gaagtagttt agttctcata acattattaa tttaagttag tgtatctaaa
     4381 attctcggaa acaaaaataa tcaataccca ttatgatagg taagcatttg gtaaaaattt
     4441 gcaggcaaat cgtaaaaatc ttgtttacgc tcgattttaa ttcaaatttt tcatatcgat
     4501 ttttttctcc cgtcaatcga acagacaata catcttggtt gtaaattaaa ttaatacttt
     4561 gattatcaga taaatgaata aaatttatta attaattctc aggaaatcat ttctttgaca
     4621 aacagtaaaa cttgtaatat cctgaaaatt tccttatctt cgcagccaat aatccttatg
     4681 cggttgtggc gtaattggta gccgcgccag acttaggatc tggtgccgaa aggcgtgggg
     4741 gttcgagtcc cttcagccgc acaaaaaaaa ccgattatca aattttattg ataaccggtt
     4801 ttttagtatc c
//
LOCUS       DAOSIB010000114         4808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_15735_length_4808_cov_10.092573, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000114 DAOSIB010000000
VERSION     DAOSIB010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15735_length_4808_cov_10.092573"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..1154)
                     /locus_tag="PLD44_08225"
     CDS             complement(219..1154)
                     /locus_tag="PLD44_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99348.1"
                     /translation="MKKVWLFFFVISFLIASCNKISDKPVSEKLSTDELSKAIKSDTL
                     FADFYEEIRKEVDDMSDIKKATYNDVTYRRLFQYVKFLQDTTYWNPLYEKWNEEWERE
                     YGVYLPKADSVINYWKKYLDENSLDKYVKVELAKIDKEYYSYSYDIRDVDLGFRLTPL
                     QGAIEQIVFTYGYQAKIHEDSKYYEKHRCRSTTPFFTPTIRYWEVDYSDRKLFSGHTV
                     ETFLRDYNLYIDVTHIRKDGINISTNDYNIPEEVSDYLKFGENDDIMQDYYKEKIIKE
                     LINKDYLRCWEYYEKQADVLKEKKDKLCFDFLKEL"
     gene            complement(1166..1471)
                     /locus_tag="PLD44_08230"
     CDS             complement(1166..1471)
                     /locus_tag="PLD44_08230"
                     /inference="COORDINATES: protein motif:HMM:NF025156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HOF99349.1"
                     /translation="MKKLLLIAVIGLLSYSCTSKWEYKTVSVKGEEQETMGKFKTNTF
                     DVSDESLNLFGNEGWELVGIYEKTETAHPNFGNEEYVSGLQPNVRTAEINFVFKRKK"
     gene            complement(1482..2045)
                     /locus_tag="PLD44_08235"
     CDS             complement(1482..2045)
                     /locus_tag="PLD44_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99350.1"
                     /translation="MKRVIFLPSLIVMSMCFQLQAQDTQKEAENAAKTKMELFSSKTG
                     VITKFIDTQLPNLSALYTSAETRIRKVKSGNTETYFYQIEKEGKYGSSTASIEYSDLL
                     EVIKAVKTLKSEVETDIASNPDYMENKFTTVDGFQVGYYISKGKATWYIRLEKYGSDK
                     TLFVTDGEIIETAFTNAKNKIDELKTK"
     gene            complement(2750..2926)
                     /locus_tag="PLD44_08240"
     CDS             complement(2750..2926)
                     /locus_tag="PLD44_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99351.1"
                     /translation="MNAVKATDISSQVEESVKTLVRKIQGTRSTAKKTETQKADMTAE
                     GKEVKEISTLQDVL"
     gene            complement(2937..3530)
                     /locus_tag="PLD44_08245"
     CDS             complement(2937..3530)
                     /locus_tag="PLD44_08245"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HOF99352.1"
                     /translation="MKKELSRIKKNLTPYNGVIYFVVILLICHFVWKLLVRGDDSNRQ
                     VTFLGNDISAPFQFMANHVAQTTASVLNFLGFHIQLFANNVLKQENGFGIQIVWSCTG
                     LKQAYIFFCIIAFYCGPMRKKLWFIPLGLAVVYVFNIFRIAAITALVQQHSSWFTFLH
                     EYFFKYLFYAVIFALWVLWEEKFVTKKEKNETHMTQT"
     gene            complement(3538..4569)
                     /gene="tsaD"
                     /locus_tag="PLD44_08250"
     CDS             complement(3538..4569)
                     /gene="tsaD"
                     /locus_tag="PLD44_08250"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOF99353.1"
                     /translation="MNPIIILGIESSCDDTSAAVLRDGVMLSNVIANQSVHAKFGGVV
                     PELASRAHQQNIIPTVQEALRQADIDKKQLSAVAFTRGPGLLGSLLVGTSFAKGFAQA
                     LGIPIIDVNHLQAHVLAHFIRKDENDNSPQPQFPFLCLLVSGGNSQIVLVKAYNDMTV
                     VGQTIDDAAGEAFDKCAKVMGLPYPGGPHIDRLAKEGNPNAFRLNKPQIEGYSYSFSG
                     LKTSFLYLLRDKMKENSNFIEENLNDLCASLQATVVDILMNKLKKAAKDYNIHQIALA
                     GGVSANSALRQAFNDLATFDGYEVFIPPFAFTTDNAAMIAMTGYFKYLDHDFCSFDDV
                     PFARVSNTF"
BASE COUNT         1498 a          908 c          757 g         1645 t
ORIGIN      
        1 ttttcaaatt tactaatatt tcttgtgtag tgtcctctta gcttgcaggt aacggctggc
       61 aatatgacca gtggcggaat gcgggcgact tccctgtctg ccgttacaaa acttgatgcg
      121 ggcacgaaac tttcaagtta caactgtgcc cgacattggt tatattgcgt gttaactgct
      181 ggtgtttctt tatttcctgt gtttttcaaa aactgtttct aaagttcttt caagaaatcg
      241 aaacaaagtt tatccttttt ctcttttagc acatcagctt gtttctcata atattcccaa
      301 catcttaaat agtctttgtt tatcaactct ttgataattt tttctttata ataatcctgc
      361 ataatatcat cattttcgcc gaacttcaga taatctgaaa cttcttcggg tatattataa
      421 tcattagtgc taatatttat tccgtcttta cgaatatgag taacatcaat gtataaattg
      481 taatctctta aaaatgtttc aactgtatga cctgaaaata attttctatc agaatagtca
      541 acttcccaat atcttattgt tggagtaaaa aatggagtag tagaacgaca acggtgtttc
      601 tcataatact tgctatcttc atgtatttta gcttgataac cgtaagtgaa cactatttgc
      661 tctattgctc cttgtagtgg agttagtcta aaacctaaat ctacatctcg aatatcataa
      721 gaataagaat aatattcttt gtcaatcttt gccaactcaa cttttacata tttatccaaa
      781 gaattttcat ctaaatattt tttccaatag tttatgacag aatctgcttt tggtaaataa
      841 actccatact ctctttccca ttcttcgttc catttttcat ataaaggatt ccaataagtc
      901 gtatcctgca aaaactttac atattgaaac aatcttcggt aagtaacatc attgtatgtt
      961 gcttttttta tgtcgctcat atcatcaact tcttttctga tttcttcata aaaatcggca
     1021 aatagggtgt cgctttttat tgctttggat agttcgtccg tacttaattt ttcagaaaca
     1081 ggtttgtctg aaattttgtt acaacttgct atcaaaaaag aaattacaaa aaagaataac
     1141 catactttct tcataaagcc tccttttatt tcttacgttt aaaaacaaag ttgatttcag
     1201 ccgttcttac atttggttga agacctgaaa catattcttc gttgccaaaa tttgggtgtg
     1261 ctgtttcagt tttttcgtaa atgccgacta actcccaacc ttcatttcca aacaagttta
     1321 acgattcatc agaaacatca aatgtattgg tcttaaattt ccccattgtt tcctgttctt
     1381 ctcctttaac ggagactgtc ttatactccc atttagaagt acacgaatag gacaagagtc
     1441 ctattactgc aattaaaagt aactttttca tatacaactg attatttggt ttttaattcg
     1501 tcaattttat tttttgcatt tgtaaatgct gtttctatta tttcaccatc ggtaacaaac
     1561 aaagttttat cagagccata tttttcaagt cggatatacc aagttgcttt ccctttggaa
     1621 atatagtaac ctacttgaaa accatcgacc gtagtaaatt tgttttccat ataatcagga
     1681 ttgcttgcaa tatctgtctc gacttcactt tttaaagttt ttactgcttt aattacttca
     1741 agcaaatccg aatattcaat agaagcagtt gaagaaccat atttgccctc tttttcaatt
     1801 tggtaaaaat aagtttcggt gtttccactt tttacttttc taattcgagt ttcggcactt
     1861 gtgtacaaag cacttaaatt aggaagttga gtatcaataa attttgttat gacacctgtt
     1921 ttggaagaaa aaagttccat ttttgttttg gcggcgttct ctgcttcttt ctgtgtgtcc
     1981 tgcgcctgca actgaaaaca cattgacatc acaattaaac ttggtaaaaa aattactctc
     2041 ttcatttttt aatcccttat tcgtgtcggg cgcaactttt attttcctac tctaacggct
     2101 ttgcaggtct tgcctcttgt tattgcaaat gctttttatc cgttttctgc tgacgatagc
     2161 tcactctaca cttgcagtta actcatttat acacgctact tcatagcacc ttatttccat
     2221 atttggatgg atagacgcta ttatgttgcg ttttatcttt cgattcaaaa ttacaaaaac
     2281 ttttcattta ataaacaaaa tttatgaagt ttttttcaca atccccgtcc taccggatat
     2341 cctcattcaa tatacaaagc tttgcgggcg tctgaggtga accaacggtc atgacagaag
     2401 agaataattg caatcacctc gtaatgtatt ataaaggttt gtaatccgaa acactttcca
     2461 cggcaaaata tatttattga agcaaagata actttttaat ctaaatattt aacttagcgg
     2521 attataaatc cttataatac aagaagaggg tgtctcaaaa ggaattctca aaatgcaaaa
     2581 acttacaatt taaaacctac tgacaaagaa aactcccaat acagcaaaat aattttgttg
     2641 aatttattct gttttgggga tttttcatac aattactaac agattgaaag tcaattttct
     2701 aattttggtc attatgagac aacctgcgac gaacgccaac tttagtcatt taaagaacat
     2761 cctgtagggt ggatatttct ttgacttctt ttccttcggc tgtcatgtcc gctttttgcg
     2821 tttctgtttt ctttgcagtg gatcttgttc cctgaatttt tcgaaccaac gtttttacgc
     2881 tttcttctac ttgggaagag atatctgtgg ctttaacggc gttcattact cgggtgtcaa
     2941 gtttgtgtca tgtgggtttc attcttttct tttttagtta caaatttttc ttcccacaaa
     3001 acccataaag caaaaattac agcataaaat aagtatttga aaaaatattc gtgcaaaaaa
     3061 gtaaaccaag aagagtgttg ctgaacaaga gccgttattg cggcgattct gaaaatatta
     3121 aacacataaa ccacagccag tccgagcggg atgaaccaca atttttttct catcggccca
     3181 caataaaaag ctatgataca gaaaaaaata taagcctgtt tcaggccggt acacgaccat
     3241 acaatttgga taccgaagcc attttcctgt ttcagaacgt tattggcaaa aagctgaata
     3301 tgaaatccta aaaaatttaa aacgcttgca gtagtttgag caacatggtt ggccataaat
     3361 tgaaatggcg ccgaaatatc attccccaaa aacgtaacct gacggtttga atcatcaccc
     3421 cgtaccagta atttccatac aaaatgacaa attagcaaaa taacaacaaa ataaattaca
     3481 ccgttgtaag gagtcaaatt tttttttatt ctggaaagct cctttttcat aatattctta
     3541 aaaagtattg ctaactcttg caaacggtac atcgtcaaag gaacagaaat cgtgatcgag
     3601 atacttgaaa taaccggtca tggcaatcat ggcagcattg tcggtagtaa aagcaaaagg
     3661 aggaataaaa acttcataac catcaaaagt tgccaaatcg ttaaaagcct gacgcaaggc
     3721 tgaattggct gaaacacctc cagccagtgc aatttgatga atgttgtaat cctttgccgc
     3781 ctttttcagt ttattcatca aaatatcgac aacggttgcc tgcagagaag cacacaaatc
     3841 attgagattt tcctcaataa aatttgaatt ttccttcatt ttgtcccgaa gcaagtacaa
     3901 aaaagaagtt tttaaaccac taaaactgta gctgtagcct tcaatttgcg gtttattcag
     3961 tctgaaagca tttggatttc cttcttttgc cagacggtcg atatgagggc caccgggata
     4021 aggcaaaccc ataacttttg cacatttgtc aaaagcttca cctgcggcgt catcaatggt
     4081 ctgaccgaca acggtcatat cgttataagc ttttaccaga acaatttgag aatttcctcc
     4141 cgaaacaagt agacacaaaa aagggaattg aggttgcgga gagttatcgt tttcatcttt
     4201 tctgataaaa tgagccaaaa cgtgtgcctg caaatgattt acgtcaataa tgggaatgcc
     4261 gagtgcctga gcaaaacctt ttgcaaaaga agttcccaca agcagtgagc ccaataaacc
     4321 cggtcctcgg gtgaaagcta cagcacttaa ttgttttttg tcgatgtcgg cttgtcttaa
     4381 tgcttcctga acggtaggaa taatattttg ctgatgcgca cgagatgcca attccggaac
     4441 tactcctcca aatttggcgt gaaccgactg attggcaata acatttgaaa gcatgactcc
     4501 atcgcgcaaa acggctgccg aagtgtcgtc acaggaagat tcgattccta atataataat
     4561 ggggttcata aaaattcatt ttttaataaa aaacaacatt ctcaatcaga aatagtacgc
     4621 ttgaatttta taaaaaaata agggttgttc tacttttttg attcagaaaa tcataaataa
     4681 tttttttatt tttgtaaaat aaaattcaaa ggtataaaaa aatcagttaa aatagcaggt
     4741 tatacaattg gaggaattat tttattcctt gcaattttac ttattttgtt gcaaaacagc
     4801 agaataca
//
LOCUS       DAOSIB010000115         4769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_15903_length_4769_cov_13.168434, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000115 DAOSIB010000000
VERSION     DAOSIB010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15903_length_4769_cov_13.168434"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1334)
                     /gene="metK"
                     /locus_tag="PLD44_08255"
     CDS             complement(54..1334)
                     /gene="metK"
                     /locus_tag="PLD44_08255"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOF99354.1"
                     /translation="MAYLFSSESVSEGHPDKIADQISDALLDNFLALDRDSKVACETL
                     VTTGQVIIAGEVNTTAYVDVQSVARRVINRIGYTKSDYMFDGNACGILSALHDQSPDI
                     QRGVEREDPIEQGAGDQGMMFGYACNETSNYMPVALDLAHALVGELAKIRKEGKQMTY
                     LRPDAKSQVTIEYDDSNRPLRVHTIVISTQHDEFIQPSLTKSQDEADREMLLIIRQDV
                     QNILIPRVKLLDKQFAELFKDNFTLLVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKG
                     AHGGGAFSGKDPSKVDRSAAYAARHIAKNMVAAGVADEVLVQVAYAIGVADPINLYID
                     TFGTSKVKFSDSEIAEKINNIFDMRPKAIEERLKLRYPIYEETASYGHMGRTPMEVRK
                     VFKDGNGNQITKTVELFTWEKLDKVKEIKSIFQL"
     gene            1989..2270
                     /locus_tag="PLD44_08260"
     CDS             1989..2270
                     /locus_tag="PLD44_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HOF99355.1"
                     /translation="MNSLQIVGSLASLLSILNQFPQAIKVFKTKDTHSISLTMYCIVV
                     VCITLWLVYGIMLKDGPLIWANGLSLFPITYIFIVKLTNTIKGKDKLSI"
     gene            complement(2971..3390)
                     /locus_tag="PLD44_08265"
     CDS             complement(2971..3390)
                     /locus_tag="PLD44_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOF99356.1"
                     /translation="MYYSIAYSQQYLIQHSIKPSVQRTAVMDYLLNNRTHPTVDEIFS
                     VLSPSMPTLSKTTVYNTLKIFIERGAARMLTIDEKNARYDVDTTPHAHFICQQCGKVS
                     DVFDVRPEVFQLPLNDTFKVNSVEIFYYGVCNNCSSK"
     gene            complement(3563..4504)
                     /locus_tag="PLD44_08270"
     CDS             complement(3563..4504)
                     /locus_tag="PLD44_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOF99357.1"
                     /translation="MSKVSVIGAGNVGATCVNVLSFYQIADEVVMLDVKQGIAEGKAL
                     DINQTATLLHFNTRISGCTNDYEKTANSDVVVITSGVPRKPGMTREELVDVNAGIIKS
                     VVENVLKYSPNAILVIVSNPMDTMTYLTYKIAGLPRNRVIGMGGTLDTARFKCYLGKA
                     LNANQNEIEGFVIGAHGDTSMIPLVRYAAYKCRPVTDLLDKETLDKVVSDTMLGGATI
                     TSLLGTSAWYAPGAAAAFLVEAIIHDEKKIIPCSVYLEGEYGQNDICIGVPVLIGRNG
                     VEEILDYHLNSEEKELFIKSSESLRATNAMLKDLGVI"
BASE COUNT         1594 a          924 c          743 g         1508 t
ORIGIN      
        1 attttgaatc ttaaaaattt acacagttta tgaaatattc tccattatac tttttaaagc
       61 tgaaaaatcg actttatttc tttcaccttg tcaagttttt cccatgtaaa taattcaact
      121 gtttttgtaa tttggtttcc atttccatct ttaaaaacct ttctcacttc cattggtgtt
      181 ctgcccatgt gtccatacga agctgtttct tcataaattg gatatctcaa ttttaatcgt
      241 tcctcgatgg ctttaggtcg catatcaaaa atattattta ttttttcggc aatttcacta
      301 tcagaaaatt ttacttttga agtgccaaaa gtatcaatgt aaagatttat cggatcagca
      361 acaccaatgg cataagctac ctgtaccaga acttcgtcgg cgacgccggc agctaccatg
      421 tttttagcta tgtgtcgagc ggcataagct gctgagcgat ccactttaga tggatcttta
      481 cccgaaaaag cgccaccacc atgtgctccc ttacctccat aggtatctac aattattttt
      541 cttcctgtca atccagtgtc gccatgagga cctccgataa caaattttcc cgtcggattg
      601 accagtaatg taaaattatc cttaaataat tcggcaaact gtttatcaag cagtttaacg
      661 cgaggaataa gaatgttttg cacatcctga cggataatca acaacatttc cctgtcggct
      721 tcgtcttgac ttttggtcag cgaaggttgt ataaactcat cgtgttgagt agaaataaca
      781 attgtatgaa ccctcaaagg gcggttgctg tcgtcgtact caatagttac ttgtgacttg
      841 gcatcgggac gaagataagt catttgctta ccttccttgc gtattttagc cagctctccc
      901 accaaagcat gtgctaagtc aagtgcaaca ggcatgtaat tggaagtttc attgcatgca
      961 tatccaaaca tcataccctg atcaccagct ccttgttcta tggggtcttc tcgttctact
     1021 cctctctgaa tatcaggcga ttgatcgtgt agtgcagata aaatgccaca agcatttcca
     1081 tcaaacatat aatcgctctt tgtataacca atccgattaa ttacacgacg cgcaaccgat
     1141 tgtacatcaa cataagctgt ggtatttact tctccagcta taattacctg accggtagta
     1201 accaatgttt cacaagccac tttggaatca cgatctaaag ccaaaaaatt atccaacaaa
     1261 gcatctgaaa tctgatcggc tattttatcg ggatgacctt ctgacaccga ttcagacgaa
     1321 aataaataag ccatatcatt aaattattaa attacttaaa aatcaaatgt ctgaatttac
     1381 tttgtaagtt gaagagaagg cagctaaaaa aggagaaaga aaattgaatc attttagcat
     1441 ttttttacgt ggttgcaagc agccaaatct ttccacttat aaataaattc tgtgcaaaat
     1501 taatcaaaat tctttgtttt tactaaattt gtacttatgt atatgacaaa aaaattgtat
     1561 aaacatttat gttttttatt ttcagatatt taataacggg gctttgcccc aaaacccact
     1621 tacttttttg tcttgaaaca aaaaagcaag caaaaaaatt aatttagttg aagcagctgc
     1681 gttcaacata aatcaaaata aatttgattt tgttttcgct taaacgctgc tttcaagact
     1741 gtgtccactt cactcgaaaa acttacgctc gaaaagctga aatcgcccaa actcattccg
     1801 acgaaatacg tcggaactca aacaaggacg atttataaag gcttttctca cttttttcgg
     1861 ctcaccggac aaggtcaacc cgaagtgttt ggcttatatg tgctgtttta cagatatata
     1921 aaacaaatgt ttcaattttt gtcatgtgct aaaaaatttg tatctaaata tttattaaac
     1981 ttattcgtat gaattctctt caaattgtag gttctcttgc ttctcttttg tcgatattaa
     2041 accaatttcc gcaagccata aaagttttta agacaaagga cacccacagt atttcactaa
     2101 ccatgtattg cattgtggta gtatgtatca cattatggct ggtttatggg atcatgttaa
     2161 aagacggccc attaatttgg gcaaatggac tttctctttt tcccattact tacattttta
     2221 tcgttaaact taccaacacc atcaaaggaa aagacaaact ctccatttga ttattgttgt
     2281 tccgcgtaaa ttcaagtaat taatgcaact ttttaagatg gaataaacgg taagaaaaaa
     2341 tattttttca agtcaaagga ggtaaaacat tccattcctt tgtcctgata aaataaatgc
     2401 tttacttcgg gctcttccca ttaaaaagct acaaagatga aacatttaac tgtagaggaa
     2461 aggtacgaaa tttgaacagt attacaacaa ggattgaaac aaaaagatac tctattgact
     2521 accggaaaag aaaagaaaaa aaatatcgtt gccttccgat gatgcagttg taaaatctct
     2581 atcttttttc cctcaggaaa gccactaaaa aatggacaat ccccattcaa aaacagggca
     2641 tttctttgtc gaaagagtaa aattttaatt tttaaaccaa atttttaatt tacacgcttc
     2701 gaggaaaagt attacaaatt cgcagcgtta tcaactagtc caacactgat tatttgctcg
     2761 aaaagttaaa cagatttaga aaaaggatgt gattcatatt gcaggattgc aagcaacaac
     2821 ctttggtgtt gagttaagga aaaaatagca taaaacataa aaaatctgct ttcaaaaaat
     2881 caaaacatat caaaagcaga tttaaaattt aaccgaatgg tcttatccgt aaaatttctt
     2941 atccaaattc atccaaagca gataaaattc ttatttgctt gaacaattat tgcatacacc
     3001 ataataaaat atttcaactg aattgacttt gaaagtatca ttcagtggca attgaaaaac
     3061 ttccggacga acatcaaaaa catcagaaac cttcccacat tgctggcaaa taaaatgagc
     3121 atgcggggta gtatcgacat cataacgtgc attcttctcg tcgatggtca acattcgcgc
     3181 agctcccctt tcaatgaaaa ttttgagcgt attgtaaact gtagttttcg aaagtgtcgg
     3241 catagaaggg cttaaaaccg aaaaaatttc atcaactgta ggatgcgttc tgttattcaa
     3301 cagataatcc ataactgctg tccgttgaac cgatggtttt atggaatgct gaataagata
     3361 ttgttgtgaa taggctatag aataatacat aatggtttct tatttttgct gggcaaagat
     3421 acaatatact attgacactg acaagatttc aaacttgaat taaatttaaa tttatacttc
     3481 ccattcggcc tactgtttaa aaaatttaaa aggttatgag catgtcgcat cataacctta
     3541 aaaagcaaag aaaagaaaaa aattatatta cacccaaatc cttgagcata gcattggttg
     3601 cgcgcaaact ttcagaactt tttatgaata attccttttc ttcagaattt aggtgataat
     3661 ctaaaatttc ttcaacccca tttctaccta tcagcacagg aacgccgata caaatatcgt
     3721 tttgtccata ctctccttcc aagtaaacag agcagggtat aattttcttt tcatcatgaa
     3781 taatagcttc taccagaaac gcagcagctg ctccaggcgc ataccaagcc gaagtaccca
     3841 acaaacttgt aatagtagcc ccacccagca tagtatccga aactactttg tccaatgttt
     3901 ctttatcgag caaatcggta accggacgac atttataagc tgcataacgt accagtggaa
     3961 tcattgaggt gtcgccatga gctccaatga caaaaccttc aatctcattt tgattagcat
     4021 tcagagcttt acctaaataa catttaaaac gagctgtgtc gagtgttcct cccattccga
     4081 ttactctatt gcgagggaga cctgcaattt tataagtaag gtaagtcatg gtgtccatcg
     4141 gattacttac tattaccaaa atagcattgg gagaatattt taaaacattc tcaactacac
     4201 ttttaataat gccggcatta acatctacca attcttcacg tgtcataccc ggtttgcgag
     4261 gaactcccga agtgatgact actacatccg aattggcggt tttttcataa tcgttcgtac
     4321 aaccggatat cctggtgtta aaatgaagca aagtagctgt ttgatttata tctaaagctt
     4381 taccctcagc tattccttgt ttaacatcaa gcattacaac ctcatcggct atttgataga
     4441 aagaaagaac atttacacat gttgcgccaa cattcccggc gcctatcaca cttacttttg
     4501 acatactatt ttaatttaaa ttattaatcc gagaaaagca ttgcaaaatt acaaattaaa
     4561 atgattagtg aaaaatttct ttaattattt tagcataaat atatttatct cgagaaacga
     4621 cattgttatg gcattccaaa cagaaaaatt gaacaaatct catttgaaaa taaacaaaat
     4681 aaacgtaatt ttgtagaatg taaattatat gtgattatga aacccacatg tgttgcatac
     4741 acaaacaaga atgaatataa tattcagac
//
LOCUS       DAOSIB010000116         4617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_16528_length_4617_cov_8.436212, whole genome shotgun sequence.
ACCESSION   DAOSIB010000116 DAOSIB010000000
VERSION     DAOSIB010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16528_length_4617_cov_8.436212"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..1560
                     /locus_tag="PLD44_08275"
     CDS             247..1560
                     /locus_tag="PLD44_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009436988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOF99358.1"
                     /translation="MIEKPPVLDKKKLVDVWMILNSPGHDTLFNKINNEYLYWDKVKY
                     KTPSGVAPSVFWHAVKTKRNLTAKTLTFGKNTFKFSVTEKMQELLHTLDLNLGGSLGT
                     QGIIPEKDKKFYLINSIMEEAIASSQMEGASTTRKVAKDMLRKQLKPKNKGQQMIYNN
                     YVTIQYLVENQSKDFSVEQLKEIQRLITHNTLTNKEYEGSFRTSDDVMVIDGISGEIA
                     HTPPSHQKVEQWITELCDFANNDNEEVFIHPIIKGIIIHFMLAYIHPFVDGNGRTARS
                     LVYWYMIKKGYWLTEYLSISRIIYRNKKKYEMAFLYTENDENDLSYFIQYNLEVMHMA
                     FEELKIYLQRKISEQNDLLYFKEISEINERQAQILKTLTEKPKSIFTAKELTSLFNVS
                     IKTTRSDLQQLVAMEFMQEININQRQFGYIRADNFEEKIEEARGN"
     gene            complement(1656..3080)
                     /locus_tag="PLD44_08280"
     CDS             complement(1656..3080)
                     /locus_tag="PLD44_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99359.1"
                     /translation="MRPKLSIFTDFANTLFPHELEYLMAVQNFSKPLNLKILKQIYSN
                     NTSSGKQRKPFDESIDKRTYSYLKKWITETLLKIDVDYFFDWLIETEKKILTDVIVPS
                     DETVILSNMNKMHPTNYNYIRFYELLQYYRDYLVVRSRTRHNKVVTDFLEVHHEQYHK
                     LKNLNIKLDDVTARIVKKTEFHPEEKEYFENFLWEIFFDETLDGYTRYRAVVRLTIHY
                     YNNREFEKQAVVYQHLDGYFKTPVFYSKRILSNYYANRTMMHSKLNELEEAEKYGYLS
                     IQNKNSDYLFYLINLCGVLLKQEKNSEALKLMRKSIPELKNTNNNYYKIGFASFYILT
                     FIANKQIEKAVHYASQYFQTYRKEIFEHRWHLYFTTYLFALINAEKYDKVVSICKRYN
                     LLVKEKQRIDLPDYLPIIENYYYLSEYMEGKIDQDKLISSIVKSVKPLMKNNYRSRRM
                     LDMIDELGEFLYDEMRKVKTQLLE"
     gene            complement(3315..4274)
                     /locus_tag="PLD44_08285"
     CDS             complement(3315..4274)
                     /locus_tag="PLD44_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF99360.1"
                     /translation="MKIKLLSILIMAIGFGACSTPYSALKDISSFEELDYKYPVKKVY
                     LPESEFTIAYTDEGKGDNTIILIHGLGSYLRAWEKNIGELSKTNRVIAIDLPGYGKSS
                     KEPHSGMMTFYAGIVNELVKELKLDKVVIGGHSMGGQIGMTTYLQYPQIVKGLVLVAP
                     AGFERFTKGQKQWFRDVMTVDGVRLTTPEAIQNNLATNFYRVPKDADFMISERMQMRS
                     ADEFIPYCYAVVQSVYGMVGEPVIDYLQDIKVPTLIFFGEKDNLIPNRYLNPGRTADI
                     ANYGASKIPNNKLVMIPKTGHFLMFEKAEIFNRETIEFLKGLK"
     gene            complement(4298..>4617)
                     /locus_tag="PLD44_08290"
     CDS             complement(4298..>4617)
                     /locus_tag="PLD44_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99361.1"
                     /translation="GLTYANLSASKGFIPNRLNAKVGVSAGISNVQPAGGGAIIGTEA
                     NGNIQYNFGPYMSLELHGAYLLLGDFYDSKQSAYGSPVNGGIDGRPANPWTAFLVFKW
                     LMF"
BASE COUNT         1426 a          859 c          858 g         1474 t
ORIGIN      
        1 gcctatttgc ctaacaaacc gctccgaagt taaaaaaaca ggagaaaacg caaaagcttc
       61 ttagcgtttt ttacgaattt ataaaatatt gtaaaacgtg actacgatca tggccatttt
      121 gacggcggta ctatgggaat cggcaaataa gccaatcggg attttaagat aattagggga
      181 aattagagga aaatttcccc taatttaaaa taatgtacta tctttgtgct ctaaaagaat
      241 aaagatatga ttgaaaaacc acctgtttta gataaaaaaa agcttgtgga tgtttggatg
      301 atattaaaca gtcctggcca tgatacatta tttaataaga ttaataatga atatctctat
      361 tgggataaag tgaagtacaa aacaccatcc ggagttgctc cgtctgtatt ctggcatgct
      421 gttaaaacaa aacgaaatct gacagcgaaa acacttacat ttggtaaaaa cacctttaaa
      481 ttttcagtaa ctgaaaaaat gcaggaactg ctgcatactt tagatttaaa tcttggtggt
      541 agtctcggaa cacaaggaat tattccggaa aaagacaaga aattttactt aataaattcc
      601 ataatggaag aagcgattgc ttcaagtcaa atggaagggg cttctactac gcggaaagtt
      661 gcaaaggata tgttgcggaa acagcttaaa cctaaaaaca aggggcagca aatgatttat
      721 aacaactatg ttactattca atatttagtt gaaaaccagt cgaaagattt ttcagtggag
      781 caattaaaag aaattcagcg ccttattaca cacaacacgc ttaccaataa agagtatgag
      841 ggcagttttc gcacttcgga tgatgtgatg gtaatagatg gtataagcgg agaaatagct
      901 catacgccac cgtcacatca aaaagtagaa cagtggataa ctgagttatg tgactttgcc
      961 aataacgaca atgaggaggt ttttattcat ccaatcataa agggtattat tatacatttt
     1021 atgctggcat acatacaccc gtttgtggat ggaaacggaa gaacagcacg ttcattggtt
     1081 tattggtata tgataaaaaa agggtattgg ctcactgaat acctttctat ttcaaggatt
     1141 atttatcgca ataagaaaaa atacgaaatg gcttttctct atactgaaaa tgatgaaaat
     1201 gacctatcct attttattca gtacaattta gaagttatgc atatggcttt tgaagagctg
     1261 aaaatttatt tacagcggaa aatcagtgag caaaatgatt tgctttattt caaagagata
     1321 tcggaaataa acgaacgtca agcacaaatt ctgaaaaccc ttactgaaaa accaaaatca
     1381 attttcacag caaaagagct gacatcacta tttaatgtgt cgattaaaac cacccgaagc
     1441 gacttgcaac agcttgtagc aatggagttt atgcaagaga taaatataaa tcaacgacaa
     1501 tttggctaca tcagagccga caactttgaa gagaaaatag aggaagccag aggaaattag
     1561 aggaaaattt cccctaattt tccattttac tgctcttgtg cttgatagaa atagattgtt
     1621 gggatgtgtt gaatattcga taactgctcc cttatttatt ccaataattg tgtttttacc
     1681 ttcctcattt catcgtatag aaattctccc agctcatcaa tcatgtccag cattcggcga
     1741 gagcgatagt tgtttttcat tagtggtttt acggacttaa caatggatga aattaatttg
     1801 tcttgatcta tttttccctc catatactcg gaaaggtagt aatagttttc tataataggc
     1861 aaataatcag gtaggtcaat acgttgtttt tctttaacca agagattgta gcgcttgcag
     1921 attgaaacta ctttgtcgta tttttcggca ttaatcagtg cgaacagata ggttgtaaag
     1981 tacaaatgcc aacgatgctc aaatatttct ttccggtaag tttgaaaata ttgagaagca
     2041 taatgtaccg ctttttcaat ttgtttgttg gcaatgaaag taagtatgta aaacgatgca
     2101 aaaccgattt tataataatt attgtttgtg tttttcagct caggaataga tttgcgcatt
     2161 aacttcagag cttcactgtt tttttcctgt ttcaagagta ccccacacaa attaatgagg
     2221 taaaagaggt aatcactgtt cttgttttgt atggaaaggt aaccgtattt ttccgcttct
     2281 tccagttcat taagtttgga atgcatcatg gtacgattag cataatagtt tgacagaata
     2341 cgtttcgagt agaatacagg tgttttaaaa tacccgtcaa gatgttgata tacaacagcc
     2401 tgcttttcaa actccctgtt gttgtagtaa tgaatggtga gcctaaccac cgcccggtat
     2461 cgcgtgtatc catccaaggt ctcatcaaaa aatatttccc aaagaaaatt ttcgaaatat
     2521 tctttttctt ccggatggaa ttctgttttt tttacaattc ttgccgtgac atcgtcgagc
     2581 ttaatattca gatttttaag tttgtgatat tgttcatgat gaacttcaag aaaatcagtc
     2641 accaccttat tgtgtcttgt acggcttcgt accactaagt aatcacggta atattgtaaa
     2701 agttcgtaaa accggatgta attatagttt gtgggatgca ttttattcat gttgctcaaa
     2761 atcaccgttt cgtctgaagg cacgatcacg tcagtcaaga tctttttctc agtctcaatt
     2821 agccaatcga aaaaataatc gacatcaatt ttaagcaagg tttctgttat ccattttttc
     2881 aggtaggaat aggtgcgttt atcgatactc tcatcgaagg gtttccgttg tttcccactc
     2941 gatgtattat tagagtatat ttgtttcaat atctttaaat tcaatggttt ggaaaaattc
     3001 tgcacagcca tcaaatactc taattcatgc gggaaaagtg tattggcaaa atcggtaaat
     3061 attgaaagct tgggacgcat tttaagttgt ttttagggct ctaaaggtac aaaaaaagat
     3121 ggtgtctcag aagtgcccaa ctgtttcgtt acttcgatat tcggactcag tcacgtattt
     3181 tatgtacgct ccttcgccct caatctcagt gccttgcatt tagacacttt tgagacacct
     3241 taccagtttt ttgttaaatg tgacttttgc ttttgagaaa acctctttac caaatataat
     3301 tattaaccat cctattattt caagcctttt aaaaactcaa tcgtctctct gttaaatatt
     3361 tcagcttttt caaacatcag gaaatgtccc gtttttggaa tcattaccag tttgttattt
     3421 ggaattttgg atgccccata attggcaata tccgccgtac gtcccggatt caaataacga
     3481 ttcgggataa ggttgtcttt ttctccgaag aatataagtg tcggtacttt gatatcttgc
     3541 agatagtcaa tcacaggctc acccaccatt ccgtaaacag actgtaccac tgcataacaa
     3601 taggggataa attcatctgc agagcgcatt tgcatgcgtt cggaaatcat aaagtcagca
     3661 tctttcggta cgcggtaaaa gttggttgcc aggttgtttt gaatggcttc gggagtggtg
     3721 agtcgtaccc cgtcaacagt catcacgtcg cggaaccatt gtttttgccc ttttgtgaaa
     3781 cgctcaaaac cggcgggtgc caccaacaca agtcctttta caatttgagg atattgcaga
     3841 taagttgtca taccgatttg tccacccatg gagtgaccac cgataaccac tttatccagt
     3901 ttcagttctt tgacaagttc attcacaatt cctgcataaa aagtcatcat gccgctgtga
     3961 ggctccttgc ttgattttcc gtagcccgga aggtctatgg ctattacacg gttggtttta
     4021 cttagttcac ctatgttttt ctcccaggcg cgaagatagc tacccaaacc gtgtataaga
     4081 ataatggtgt tatcaccttt tccctcgtcg gtatatgcaa tggtaaattc actttcgggc
     4141 agataaactt tttttaccgg atatttgtaa tccagctctt cgaaagaact aatgtctttg
     4201 agagcagaat aaggtgtgct gcacgctcca aaaccaatag ccataattag tatacttaat
     4261 aatttaattt tcatgttttg tttctccttt ccttgtttta gaacatcaac catttgaaaa
     4321 ctaaaaacgc tgtccatgga tttgccggac ggccatctat tccgccgttc accggacttc
     4381 cgtaggccga ttgcttgctg tcgtagaaat ctcccagcaa aaggtatgca ccatgcagtt
     4441 ccaggctcat gtacggtccg aaattatact gaatatttcc attggcttcc gtaccgataa
     4501 ttgctcctcc accggccggt tgaacattac ttattcctgc agaaactcct acttttgcat
     4561 tcaatctatt gggaataaag ccttttgatg cgctcaaatt agcataagtc aaaccgt
//
LOCUS       DAOSIB010000117         4402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_17536_length_4402_cov_7.682080, whole genome shotgun sequence.
ACCESSION   DAOSIB010000117 DAOSIB010000000
VERSION     DAOSIB010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17536_length_4402_cov_7.682080"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1159)
                     /locus_tag="PLD44_08295"
     CDS             complement(<1..>1159)
                     /locus_tag="PLD44_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOF99362.1"
                     /translation="NMVQHYTTRYFYEFAEPVTGMARERSNDRNGEIVTTGGTGFGIM
                     VLIAGAERNYFSRQDARRSITKIVSFLEKAERFHGAWAHWYDARTGKPFSFSKYDDGG
                     DLVETAFLVQGLLAAREYFNTENVEEHLLSNRISQLWETVEWNWYTQGTDSLYWHWSK
                     NYGWKMNHRIKGFDETLITYVLAASSPTYPIEKKVYDACFKNSPYYFNGKSYYGIKLD
                     LGMEYGGPLFFTHYSFLGLNPNGLSDDHTDYFERNKAHTLIHRAYAIDNPKKHTDYGA
                     KLWGFTSSDDPMAGYSSHHPGTDADNGTVSPTAALSSIVYTPEESLEVLRYLYFEKGK
                     MLFGKYGFYDAYNPGMVEGQQTVKSYLAIDQGPIAVMIENYRSGLLWKLFM"
     assembly_gap    1160..1259
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1434..3662)
                     /locus_tag="PLD44_08300"
     CDS             complement(1434..3662)
                     /locus_tag="PLD44_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOF99363.1"
                     /translation="MKNYLSSLVIISFLLMGCTTATQTSTADKKIDAKVDSVLKLMTL
                     DEKIGQMVLYTSDWDVTGPTLKSDYIDDIRSGKCGNIFNAHTVEYVRKLQKIAVEESR
                     LGIPLLFGYDVIHGHKTIFPISLGESASWDLQAIEKSARIAATEAGASGLNWTFAPMV
                     DISVEPRWGRVSEGAGEDPFLGSQIAAARVRGFQGNDLADTTTVLACVKHFAAYGAPL
                     AGRDYNTVDMSQRVFLDVYLPPYKAAIDAGALSVMSSFNEFDGVPATGNKYLLTDILR
                     NQLGFKGFVVSDYTSVNEMVHHGIVANEEEAALLAIKAGLDMDMQGEVYAKYLKGLLE
                     KGLVDEKTIDQSVRRILKVKFKLGLFDDPYRYCNEAREKRTIYAPEHLEAAFDVAKRS
                     MVLLKNDNNVLPLKKGEKIAVIGELAASKRDLLGSWKAAGDWNFMKSVLDELKEYNGE
                     KNVLYAEGCKKMGDDRTGFAQAIATARRADKIVMVIGEDAEWTGEAASRSNINVPGVQ
                     TELLEQLKLLNKPLVVVLMNGRPLVLTKENKLADAILEAWFPGTMGGKAITYVLYGEY
                     NPSGKLTMTFPQNLGQVPIFYYEKNTGRPIYLSNDKYKSKYLDCPNDPLFPFGFGLSY
                     TTFEYSDITLSTKELQQNGSIKATVTVTNTGKTDGEEVVQCYIRDMVGSVTRPVKELK
                     GFEKIFLKAGESKEVSFTITPDLLAFHRLDMTYGTEPGDFKLFIGGNSRDVKETSFKL
                     ID"
     gene            4098..4289
                     /locus_tag="PLD44_08305"
                     /pseudo
     CDS             4098..4289
                     /locus_tag="PLD44_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
BASE COUNT         1278 a          914 c          781 g         1329 t
ORIGIN      
        1 ttcataaaaa gtttccacaa aagaccactt ctgtaatttt caatcattac ggcaattggt
       61 ccctgatcga tagccagata acttttgacg gtttgctgtc cttcaaccat tccaggattg
      121 taggcgtcat aaaatccata ttttccaaac agcatttttc ctttctcgaa atataaatat
      181 ctcaatactt ccaacgactc ttcgggagta taaactattg aagacaaggc agccgtagga
      241 gaaaccgttc cgttgtcggc atcagtaccg ggatgatggg aagaatagcc tgccattgga
      301 tcatcagaag atgtaaaacc ccatagcttt gccccataat cggtatgttt ttttggatta
      361 tcaatggcat aagctctgtg aatcagggta tgagctttgt ttcgttcaaa ataatcggta
      421 tggtcatcgc ttaaaccatt gggattcagt cccaaaaagc tgtaatgggt aaaaaacaat
      481 gggcctccat attccattcc caaatctaac tttattccgt aataactttt tccgttgaaa
      541 tagtaaggag agtttttaaa acaggcatca tacacttttt tttcgattgg ataagtgggt
      601 gaggaagctg ccagtacata agttatcagc gtttcgtcaa agcctttgat gcgatgattc
      661 attttccaac cgtagttttt cgaccagtgc cagtaaagcg aatcggtacc ttgtgtatac
      721 caattccatt ctactgtttc ccaaagttgt gagatgcgat tcgacaacaa gtgttcttca
      781 acattttcgg tgttgaaata ttcccgtgca gccagcagac cttgtaccag aaatgcggtt
      841 tctaccaaat cgcccccatc atcatatttt gaaaagctga agggttttcc cgttcgtgcg
      901 tcataccaat gcgcccatgc tccgtgaaat ctctcggctt tttccaaaaa ggaaacaatt
      961 tttgtgatgg aacgacgggc atcctgccga ctgaaataat ttcgttctgc gccggcaatc
     1021 aataccataa taccaaaacc agttccgccg gtagttacaa tttctccatt tcggtcattg
     1081 ctcctttcgc gcgccatgcc ggtaacaggt tcggcaaatt cataaaaata tcttgttgtg
     1141 tagtgttgta ccatgttcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     1261 cgtgtgcggg ccacgcttct gctttcattg tagaaggtac tgtaaaattt tgttgagtgc
     1321 caccccacac tgttatttca acgataaata ataacaataa tgaaagtagc gcttttctac
     1381 ttttcctttt caacgtgcaa tatttctgct tttctatcat gaaaaaagat tttctaatca
     1441 attagtttga atgaagtttc ttttacgtct ctcgaatttc caccaataaa cagtttaaaa
     1501 tctcctggtt cggttccgta agtcatatcc agtctgtgaa aagccagcaa atcgggcgta
     1561 atggtaaaag aaacctcttt actttcgccg gctttcagaa aaattttctc aaaacctttc
     1621 aactctttta ctgggcgagt tacactaccc accatatcgc gaatatagca ttgcactact
     1681 tcttcgccgt ctgttttgcc ggtattggtt acggtaacag ttgccttgat acttccgttt
     1741 tgttgcaatt ctttggttga caaagtaatg tctgaatatt caaaagtggt atagctcaat
     1801 ccgaaaccaa aaggaaacag tggatcatta gggcaatcga gatattttga tttgtattta
     1861 tcgttactca aatatatggg acgtccggta tttttttcgt aataaaatat gggaacttga
     1921 cctaaatttt gtggaaaggt catggtcagt ttgccggaag gattatattc tccgtaaaga
     1981 acataagtta tggcttttcc acccatcgtt ccgggaaacc aagcttccag aatagcatct
     2041 gccaatttgt tttcttttgt gagaacaaga ggacgaccgt tcatcaatac tacaacgaga
     2101 ggtttattta acaatttcag ttgctcgagt aattctgttt gcacacccgg tacgttgata
     2161 ttcgatcggc tggcagcttc tccggtccat tcggcatctt cgccaatcac catcacaatt
     2221 ttatcggctc tgcgggctgt agctattgct tgagcaaatc cggtacggtc gtcccccatt
     2281 tttttgcagc cttctgcgta gagtacattt ttttctccat tgtattcttt caattcatcc
     2341 agaacagatt tcatgaaatt ccaatctccg gcagctttcc acgaaccaag caaatcgcgt
     2401 tttgaagccg ccaattcgcc gattactgca attttttctc cttttttaag aggaagaacg
     2461 ttgttgtcat ttttcagtaa aaccattgaa cgtttagcaa cgtcaaaagc agcttccaga
     2521 tgttcggggg cataaatcgt tcgtttttcg cgcgcttcgt tgcaataacg ataagggtca
     2581 tcaaaaagac ccaatttgaa tttcaccttt aaaattcgac gtactgactg atcaattgtt
     2641 ttttcatcta ccaagccttt ttcgagcagt ccttttaaat atttggcata cacttctccc
     2701 tgcatatcca tatcgagacc agccttgatg gccagcaacg cagcttcttc ctcattggca
     2761 acaatcccgt gatgaaccat ttcgtttacc gaagtataat cacttactac aaagcctttg
     2821 aatccgagct gatttctcaa tatgtcggtt agcaaatatt tgtttcccgt agcaggaaca
     2881 ccgtcaaatt cattgaaaga actcataacg ctcagagcac cagcatcaat ggcagctttg
     2941 taaggaggca gataaacatc aagaaatacg cgttgcgaca tgtccactgt gttataatcg
     3001 cgtcctgcca acggtgctcc ataagcagca aaatgtttta cacaggctaa aactgttgtc
     3061 gtatcggcta aatcatttcc ctgaaaacct ctgacacggg ccgcagcgat ttgagaaccc
     3121 agaaaaggat cttcgcccgc cccctcagaa actcttcccc agcgcggctc tacagaaatg
     3181 tcaaccatgg gcgcaaaagt ccagtttaat ccggatgcac cagcttcggt ggccgcaata
     3241 cgtgctgatt tttcaatagc ttgcaaatcc cacgaagccg attcacccaa agaaatagga
     3301 aaaatagttt tgtgcccatg aatgacatcg taaccaaaca acaacggaat acctaaccgt
     3361 gattcttcta cggcaatttt ttgaagtttg cgtacatatt caaccgtatg tgcgttgaag
     3421 atgttgccac atttgccgct gcgtatatcg tctatgtaat cgctttttaa tgttggaccg
     3481 gtaacatccc aatcactggt ataaagcacc atttgtccga ttttctcatc caaagtcatc
     3541 aattttaata cagaatcaac tttggcatcg atttttttgt cagctgtgga agtttgagtt
     3601 gcagtagtac aacccataag caggaaagaa ataattacca atgatgaaag gtagtttttc
     3661 atggttcaac gttttaaaat ttcgatttca aataacgttg caaagaagga gataattaaa
     3721 agcgtttgca aatttttata ccctataaca accccactgg tgaaaatatt tttaatttat
     3781 acccctattt ttcccctata atgagaaaat attattattt aaacctttaa aaaacaaata
     3841 aatatatttt attttaattt taaaaaatat tttgtagaat tgcttgaatt aaggcaaact
     3901 ataacctaaa aatttgttaa attatggaaa gggagaagaa taaatgagtg aaacaatatc
     3961 tgttgagtgt aatttgacaa tttctaaact gatattagct tataaaaaaa accgagcagt
     4021 aaaaaataat agttattttt acaactgcga tggaaaagag aaaaaatttg acatcaaaaa
     4081 tcaaggctga aattgtttta tccttgttgc cacagggaaa agtcgaagct gcaagacgcg
     4141 taaaagcaat ggttgcaaaa atggacgaac tcggtttcgg caattgcacc aataccggag
     4201 cttgcgaggc cgaatgccca aaaaatattt caatctccaa cattgcccgc cttaaccggc
     4261 aatttctttc agccaaattc aaggattgac attctcattt attactcgat agatttcaaa
     4321 aaagaccgta agaaaaactt gcggtctttt ttgttgaaaa aatgaaaatt acatttgtaa
     4381 tttccttaaa attttctata tc
//
LOCUS       DAOSIB010000118         4370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_17725_length_4370_cov_13.514253, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000118 DAOSIB010000000
VERSION     DAOSIB010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17725_length_4370_cov_13.514253"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            362..1888
                     /gene="gltX"
                     /locus_tag="PLD44_08310"
     CDS             362..1888
                     /gene="gltX"
                     /locus_tag="PLD44_08310"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOF99364.1"
                     /translation="MRRVRVRFAPSPTGPLHIGGVRTALYNFLFARKNNGDMLLRIED
                     TDSSRFVPGAEDYIVESLKWLGITIDEGIGAEKEGAFGPYRQSERKHIYRKYVDQLLN
                     DGKAYIAFDSPIELESKRAEIPNFQYDAQTRLSMNNSLTLPEEEVRKRLENDEQYVVR
                     IKIEPGEDIVVNDLIRGEVSINSSVIDDKVIFKSVDELPTYHLANVVDDYLMQITHVI
                     RGEEWLPSSPLHVLLYRYLGWENEMPQFAHLPLLLKPDGKGKLSKRDGDRLGFPVFPL
                     NWKDPRTGEIYNGFREAGYFPEAVVNFLALLGWNPGTDQEIFSMDELIEQFSLERVSK
                     SGAKFDYEKAKWFNHKYLQMKPDEEVADIFQRILIEKGFIEDTDKVIKIISLIKDRAD
                     FITDLWEQSHYFFEAPEHYDAKAVQKRWKPEIPEQLEELGKMLLSLEAASADEMEKSI
                     KEWISGKNYHLGNLMNALRLALVGELKGPSIFEIVEVLGKEEAARRIKKAIEVLSGES
                     "
     gene            1948..3249
                     /locus_tag="PLD44_08315"
     CDS             1948..3249
                     /locus_tag="PLD44_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOF99365.1"
                     /translation="MKKVLIITYYWVPSGGAGVQRWVKFTKYLRSFGWEPVVYTPQNP
                     EYPALDHSLEKDVPPDMEVIKTPIWEPYNIYRNLMGKKNEPINAGFISENKKSGWKEQ
                     FSIWIRGNFFIPDPRRFWVKPSVKFLKNYISENQIDAVVTTGPPQSMHLIGLGLKKNF
                     PTLTWLADFRDPWTHIDFYDDLHLTKIADRIHHRLEKKVVRNADAVVVVTKGMLEEFL
                     KLQPKRIEMIPNGFDEEDFLISRPELDQKFTISHIGTLNTARNPTIFWKVLNELCAEN
                     EQFKSDLLIQLIGKVDFSILEEINENNLKDNFLKIDYLPHNEAIAKQMSSQVLLLLIN
                     QTNNAKGIVTGKLYEYLAARRPILGIGPTNGEVGEILKSSQAGIMIDYIDAKKMKETI
                     LDYYQQYKNHSLTVPSNLVENFSRKNLTRDLANLLDSLCEK"
     gene            3237..4307
                     /gene="wecB"
                     /locus_tag="PLD44_08320"
     CDS             3237..4307
                     /gene="wecB"
                     /locus_tag="PLD44_08320"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOF99366.1"
                     /translation="MRKIVTVVGARPQFIKAATLSRQFPTVGVEEIIIHTGQHFDNNM
                     SDVFFEEMEIPRPAYHLDIHNLTHGAMTGRMLEGIEKILLKEKPDGMLVYGDTNSTLA
                     GALAAVKLNIPVIHVEAGLRSFNMEMPEEINRTLTDRISSLLFCPTDVAVQNLKREGF
                     EHFPARIIKNGDVMQDAALYYASKAEEKSDILQKIGGEKFVLATIHRQENTDHPEKLR
                     NIVEGLNQIHRQVRVIVPLHPRTRKILQQLSIIPNFTIIEPVGYFDMIVLEKSCELII
                     TDSGGVQKEAFFFGKHCITLREQTEWIELVENGFNILVGSDVEKLLQAFATFQNKKSD
                     FSIDLYGNGHAAEKAAKDIAAF"
BASE COUNT         1372 a          759 c          914 g         1325 t
ORIGIN      
        1 actccgattt caagtcaaga atttacaata ggaatcattg cggataatca ttaaaaattt
       61 tgtaaaaatt aaaattttta tgcaaaacat ataatttttt tctataagat tttctttagc
      121 cggttttata tctattttgc tcttctttta ttttttaatt aattccctca tcaattaatt
      181 ttagatgtaa agtttctcaa ggttgttaat gaaaacttag attttgagtt ggatttaaaa
      241 ataattattt tccttaaact cctaatgaac attttaagtt tatatctcag ttaaatttct
      301 tatttttgca aaccgataac atatatcagt tttaaagctt caaacattaa atttcctttt
      361 catgagaaga gtgagagttc gttttgcgcc aagtcctacg gggcctttgc acattggtgg
      421 tgttcgtacg gctttgtaca attttctttt tgcccgaaaa aacaatggcg acatgctttt
      481 gcgcattgaa gataccgatt cttcacgctt tgttcccgga gctgaagatt atattgtaga
      541 gtcgctgaaa tggttgggaa taactattga tgagggtatt ggggcagaga aggaaggcgc
      601 atttggccct tatcggcaaa gcgaaagaaa acatatttac cgaaaatacg tagatcagct
      661 tttaaatgac ggaaaagcct acattgcttt tgattcgccc attgaactgg aaagtaaaag
      721 ggctgaaata cccaattttc agtacgatgc acagacacgt ctttcgatga acaattctct
      781 gactttgccc gaagaggaag tcagaaaaag attggagaac gatgaacaat atgtggtgcg
      841 catcaaaatt gaacccggag aagatattgt agtgaatgat ttgattcgtg gtgaagtcag
      901 cattaattct tcggtgattg acgacaaagt tatttttaaa tcggttgatg aattgccaac
      961 ctatcatttg gccaatgttg tggacgatta cctgatgcag atcactcacg taattcgtgg
     1021 agaagaatgg ttaccgtctt cgcctttgca tgttttgctg tatcgatatc ttgggtggga
     1081 aaacgaaatg ccgcaatttg ctcaccttcc tttgctgctt aaacccgatg gaaagggaaa
     1141 actcagtaaa cgcgacggcg acaggcttgg gttcccggtt tttcctctca attggaaaga
     1201 tccccgcacc ggcgaaatct acaatggttt tcgcgaagcc ggatattttc ctgaagcggt
     1261 agtaaatttt ttggctttgc tgggctggaa tcccggtacc gatcaggaaa ttttttcaat
     1321 ggatgaactc attgaacagt tttcgctaga gcgtgtcagc aaaagtggtg caaaattcga
     1381 ttatgaaaaa gccaaatggt tcaatcataa atatctgcaa atgaaaccgg atgaagaggt
     1441 ggcggatatt tttcaacgta ttctcattga aaaaggtttt atagaggata ccgacaaagt
     1501 gattaaaatt atctctctga tcaaggatcg agccgatttt attaccgatt tgtgggaaca
     1561 gtctcattac ttttttgaag caccggaaca ttatgatgcc aaagccgtgc aaaagcgctg
     1621 gaaacccgaa attcctgaac aactcgaaga actgggtaaa atgttgctat cgctggaagc
     1681 tgcttcggct gatgaaatgg aaaaaagcat taaggaatgg atttcgggaa aaaattacca
     1741 tctcggcaat ttaatgaatg ctcttcgtct ggctttggta ggggaattga agggcccgtc
     1801 catttttgaa attgttgaag tgctgggaaa agaagaggcg gccagacgaa ttaagaaagc
     1861 catcgaagtg ttgagcggtg aaagttaata aacggaagtt ttttagctaa aaaagctctc
     1921 atagtaaaaa ggttcttaaa ataacgaatg aaaaaagtac tgatcattac ttattattgg
     1981 gtaccgagtg gaggtgccgg cgtgcagcga tgggtaaagt ttacaaagta cctgcgaagt
     2041 tttggttggg aaccggttgt atatacgcct caaaatccgg aatatcctgc gcttgaccat
     2101 tcgctcgaaa aagatgttcc gcccgatatg gaagtgatta aaacgcctat ttgggaacct
     2161 tacaatattt atcgaaactt gatgggcaag aaaaatgagc cgatcaatgc cggatttatt
     2221 tccgaaaaca agaaatcggg ttggaaggaa caattttcca tttggattcg aggcaatttt
     2281 tttattcccg acccacgtcg cttttgggta aagccttcgg tgaaattttt aaaaaactat
     2341 atttccgaaa accagattga tgcggttgtt actaccggac ctcctcaatc catgcatttg
     2401 attggactgg gattgaaaaa aaattttcca actttaacgt ggctggccga ttttcgtgat
     2461 ccctggaccc atatcgattt ttatgacgat ttgcatttaa cgaaaattgc cgaccgcatc
     2521 catcatcgat tggaaaaaaa agttgtgaga aatgctgatg ctgtggtggt agtgacaaaa
     2581 ggaatgttag aagaattctt aaaacttcag cccaaaagaa ttgaaatgat tcccaatggt
     2641 tttgatgagg aagattttct tatttcccga ccggaattgg accaaaaatt taccatttcg
     2701 catattggca cgctgaatac tgccagaaat ccaacaatat tctggaaagt tttgaacgaa
     2761 ctttgtgctg aaaatgaaca atttaagtcc gacttactca ttcaactgat tggcaaagta
     2821 gatttttcta ttttagaaga aatcaatgaa aataatttaa aagataattt tttaaagatt
     2881 gattatttac ctcacaatga agcaattgca aaacaaatgt catctcaggt gcttttgttg
     2941 ttgattaatc aaacaaacaa tgcaaaggga attgttacgg gcaaattata cgaatatctg
     3001 gctgctcgtc gccccatttt gggaattgga ccaacaaatg gagaagttgg tgaaattttg
     3061 aagagctctc aagctggcat catgattgat tatatcgatg ctaaaaaaat gaaggaaaca
     3121 attcttgact attatcagca atataaaaat cattcattaa cagttccaag caatttagtg
     3181 gaaaatttct ctcgaaaaaa tttaactcgt gatttagcaa acttattgga ttcattatgc
     3241 gaaaaatagt tacagttgtt ggagctcgtc cacaatttat caaggcagcc acgttgagtc
     3301 gccaatttcc aacagttggt gtcgaagaaa taattatcca taccggacag catttcgaca
     3361 acaacatgtc cgatgttttt tttgaggaga tggaaattcc gcgtccggcg tatcatttgg
     3421 atatccacaa cctgacacat ggagccatga ccggccgaat gctggaagga attgaaaaaa
     3481 ttctgttgaa agaaaaacct gatggaatgc tggtttacgg tgataccaat tcaacgcttg
     3541 ccggcgcatt ggctgctgtt aagttgaaca ttcccgtgat tcatgtagaa gccggtttgc
     3601 gttcgttcaa tatggaaatg ccggaagaaa tcaaccgtac ccttactgat cgcatttcaa
     3661 gcctgctttt ttgcccaacg gatgtagctg tacagaattt aaaaagagaa ggttttgagc
     3721 attttcctgc acgaattata aaaaacggtg acgtcatgca ggatgcagct ttgtattatg
     3781 cttcaaaagc agaagaaaaa tctgatattt tacagaaaat cggtggggaa aaatttgtgc
     3841 tggctaccat tcaccgtcag gaaaataccg atcatcccga aaaattgaga aatattgtcg
     3901 agggactaaa tcaaatccat cgtcaagtac gtgtaattgt tcctttgcat ccacgtaccc
     3961 gcaaaatttt acaacaattg agcattattc ctaactttac gataatagag ccggtcggtt
     4021 attttgacat gattgtgctc gaaaaatcgt gcgaactgat tatcactgat agcggtggcg
     4081 tgcaaaaaga agccttcttt tttggtaaac attgtatcac gcttcgcgag caaaccgaat
     4141 ggattgaact ggtagaaaat ggatttaata tcttggtagg tagcgatgta gaaaaacttt
     4201 tgcaagcttt tgcgactttt caaaataaaa aatctgattt ttccatagat ttgtatggca
     4261 atggtcatgc agccgaaaag gcagcaaaag atattgctgc tttttgaatg ttatattgtt
     4321 ttgcaatatc atcttttttt gattttatct gcttcatctt ttaaaaaagc
//
LOCUS       DAOSIB010000119         4306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_18060_length_4306_cov_8.434486, whole genome shotgun sequence.
ACCESSION   DAOSIB010000119 DAOSIB010000000
VERSION     DAOSIB010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18060_length_4306_cov_8.434486"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(383..2026)
                     /locus_tag="PLD44_08325"
     CDS             complement(383..2026)
                     /locus_tag="PLD44_08325"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOF99367.1"
                     /translation="MRKLLLLIVSVLAYFNSMACTNFLVGKAATVDGSTLISYSADSY
                     FMFGSLYYSPAATYPDNAMLDIYEWDTGEYLGKIKQAERTYSVVGNMNEFQVSIGETT
                     FGGRPELVDTTAILDYGSLIYIALQRSRSAREAIKVMTDLVEQYGYRSEGESFSIADP
                     NEVWIMEMVGKGNRNKGAVWVAQRIPDDCVSAHANQARITTFPFDDKNNCLYAEDVIA
                     FAREKGYFKGKNKDFSFSDTYAPLDYLGLRICEARVWTFFRKVDPSMDSYISYIKGET
                     AQRMPLWIKPNRKISAQDVKEFMRDQYEGTELDITKGIGAGPFHSKLRCSPLTFYVDS
                     VEYVHERPVATQQTGFSFVAQMRSWLPDYIGGILWFGVDDAASSIYVPMYCGINSVPE
                     CYRAGNGSLVEYSPTSAFWIYNQVANFAYSKYSFMMQDIKKVQSKWEQDFNSLVPTID
                     KVALGMSEESAKSFLTNFSNYQAEASTAAWKKLGEYLLVKYMDGNVKMEKDGKFLQND
                     KGIPPGIIRPGYPEDFLRMMVKENPGLRVKTAEELKNRK"
     assembly_gap    2145..2244
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2637..>4306)
                     /gene="dnaK"
                     /locus_tag="PLD44_08330"
     CDS             complement(2637..>4306)
                     /gene="dnaK"
                     /locus_tag="PLD44_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOF99368.1"
                     /translation="NEIARVPFKVVRGENNIPKVDIDGHTYSPQEISAIILQKMKQTA
                     EDYLGTQVTDAIITVPAYFNDAQRKATKEAGEIAGLNVRRIINEPTASALAYGLDKTN
                     REMKIVVFDCGGGTHDVSVLELGDGVFEVKATDGDTHLGGDDFDQRIIEWLVQEFKNE
                     NSGVDLTKDPMALQRLKEAAEKAKIELSTATSTEINLPYIMPVDGVPRHLVRTLTRAK
                     FEQLVDDLVQRTIEPCRTALQKAGLTVNDINEVILVGGSTRIPAIQQAVEKFFGKAPS
                     KGVNPDEVVAIGAAIQGGVLTGEVKDVLLLDVTPLSLGIETMGGVMTKLIEANTTIPT
                     RKSEVFTTAADNQPSVEIHVLQGERPLASQNKSLGRFHLDGIMPAPRGVPQIEVTFDI
                     DANGILHVSAKDKATGKEQSIRIEASSGLTDAEIKRMKEEAAANAEADAREKERIDKL
                     NQADSLIFQTEKQLNDFGDKIPADKRATIDAALNRLKEAHRTQNLSEIDAATHELNTA
                     FQAASQEMYNAQQQAGQGAGYNGSGQQGQGGNNQEGDVTDVDFEEVK"
BASE COUNT         1231 a          903 c          754 g         1318 t
ORIGIN      
        1 ttaaaggtct ttttgttccc agatcaattc ataaacatcc cacaagctga tcaaatctat
       61 ccgtactaat gttgccaaat ggaaaaggtc tttttcgtat cggctctttt ggggggttcg
      121 tccaactcct ttgagaatat tgctaaacag ccttattgtt ttagaatcaa tcaaaagaac
      181 ttcataaaat ttaatctgaa agtcccgccg taagaccaaa tttatttccg ctttttgcct
      241 ctatattttt tatgtttgtg gtaaaacaaa aaaattatgt gccggtgaag tcatcaaatt
      301 tgggtaactt tcctttttat tataattctg tttttttttc ggacagcagc gattttcaag
      361 ataagaaaaa gtaagaggag gtttattttc ggttttttaa ttcttctgcc gttttcaccc
      421 tcaaccctgg attctccttc accatcatac gcaaaaagtc ttcaggataa ccggggcgaa
      481 taattccggg aggaatacct ttgtcatttt gaagaaattt tccgtccttt tccattttaa
      541 cgttaccatc catgtatttt acaagcaaat attcacccag ctttttccat gctgccgttg
      601 aagcttctgc ctgataattg ctgaaattgg tcaaaaacga tttagccgat tcttcagaca
      661 tccccaaagc caccttgtca atagtgggaa caagtgaatt aaaatcctgt tcccatttcg
      721 actgaacttt tttaatatcc tgcatcataa aagaatactt gctgtaagca aaattggcaa
      781 cctgattgta aatccagaat gccgaagttg gggaatattc taccagactt ccatttcctg
      841 cacgataaca ttccggtaca ctgttgatac cgcaatacat gggcacatat atggaactgg
      901 cagcatcgtc aacgccaaac cacaatattc cgccaatata atcgggcagc caactacgca
      961 tttgagccac aaacgaaaaa cctgtttgtt gagtagctac aggacgttcg tgcacatatt
     1021 ccacactgtc cacataaaaa gtaagaggac tgcatcgcaa cttgctgtgg aaaggtccgg
     1081 caccaattcc tttggtaatg tccaattcgg ttccttcgta ctgatcacgc ataaattcct
     1141 ttacatcctg agcactgatt ttacggttgg gttttatcca cagaggcatc cgctgagcag
     1201 tctctccctt gatgtaagaa atataactgt ccattgacgg atcaactttt cggaaaaaag
     1261 tccaaacccg cgcttcacaa attctcagtc ccaaataatc caaaggagca taagtatccg
     1321 aaaaactgaa atccttgttt tttcccttaa aataaccttt ttcacgagca aaagctatga
     1381 catcttccgc ataaagacaa ttgtttttat catcgaaagg aaaagtagtg attctggcct
     1441 ggttggcatg agccgaaaca caatcgtccg gaatacgctg tgcaacccat actgcaccct
     1501 tattcctatt tccttttccc accatttcca taatccaaac ttcattcggg tcggcaatgg
     1561 aaaaagattc tccttcactt cgataaccat attgctcaac caaatcggtc attaccttta
     1621 tggcttcacg ggcagatcgg gaacgttgca acgcaatata aatcaaactt ccataatcga
     1681 gaattgcagt tgtatcgacc agttcggggc gcccgccaaa agtagtttcg ccaattgaaa
     1741 cctgaaattc attcatattg ccaacaacgg aataggttct ttcagcctgc ttgatttttc
     1801 ccaaatactc acctgtgtcc cactcataaa tatcgagcat ggcattgtcg ggataagttg
     1861 ctgcggggga atagtacaga gaaccaaaca tgaaataaga atcggcagag taactgataa
     1921 gggttgaacc atcaaccgtt gcagctttcc caaccagaaa attggtacaa gccattgaat
     1981 tgaaataagc taatacggaa acaattagaa gaaggagttt tctcattttt gtgttaaatt
     2041 ttaagaatta caaatttagt ttatataaat gtaaattcaa aatcatcagg gagagaggac
     2101 acaagaaact tatagatcaa aaagaaggct aaaatctact gagannnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnggagag aggacacaag aaacttatag atcaaaaaga
     2281 aggctaaaat ctactgagag gtcaattcta atatttttcg catcagcatt atatggaagt
     2341 aaaatatttt gttaaacaat ttgttaatag tcaaagtatt aattctataa taacattttt
     2401 aagatagtga tacctcggta aataattaat aaaataggta atctgtaaag aaaatgatag
     2461 aaaaattcaa ggaaaagttt tttccttttt tttgatcgtt tttcaacaaa aggtttatca
     2521 atttctgctt aaaaaataga tgctctcttt tataaggtgc aacatactaa aaaaaccact
     2581 gtaaactaaa ttgttacagt ggtttataga tagaaattat tggttgttta taagctttat
     2641 tttacttctt caaaatccac atcagttaca tcaccttcct gattgttgcc tccttgccct
     2701 tgttgtccgg agccgttata acctgcgcct tgacctgcct gctgctgagc attgtacatt
     2761 tcctgactcg ctgcttggaa agccgtattt aactcgtgag tggcagcatc gatttccgaa
     2821 agattttgag tacgatgggc ttctttgagt cggttaagtg cagcatcaat cgtagcacgc
     2881 ttatcagccg gaattttatc gccaaaatca tttaattgtt tttcggtttg gaagataagg
     2941 ctatctgctt ggttcaactt gtctatacgt tccttttctc ttgcatcagc ttcggcgttg
     3001 gctgcagctt cttctttcat tcgtttaatt tcggcatctg tcaatcctga agaagcttct
     3061 atgcgtatac tttgttcttt tcctgtagct ttatcttttg ccgacacatg aagtattcca
     3121 ttagcatcaa tatcgaatgt tacttcgatt tgcggaactc cacgaggagc aggcataatt
     3181 ccatccaaat ggaaacgtcc taacgatttg ttttgcgatg ccaatggacg ttctccctga
     3241 agcacatgaa tttctactga aggttgattg tcggctgccg tagtaaagac ttccgattta
     3301 cgggtaggta tggttgtatt ggcttctatc aatttggtca tcacaccgcc catagtttcg
     3361 atacctaacg acaatggagt tacatccagt aacaacacat cttttacttc tcccgtcaat
     3421 acacctcctt gaatggcagc gccaattgct acaacttcat cgggatttac accttttgac
     3481 ggagcttttc caaagaattt ttcaacagcc tgctgaatag ccggaatacg ggttgaacca
     3541 ccaaccaaaa tcacttcatt gatgtcgttc acagtcaaac ccgctttttg aagtgcagta
     3601 cgacaaggtt cgattgttct ttgaaccaaa tcatcaacca gttgttcaaa tttggcgcgg
     3661 gtcaatgtac gaaccaaatg gcgaggaaca ccatcgacag gcatgatata tggcaaattg
     3721 atttcggtag aagtagctgt agataattca atttttgctt tttctgcagc ttctttcaaa
     3781 cgttgcaatg ccattggatc tttggttaaa tctacgccac tgttttcgtt tttgaattcc
     3841 tgaaccagcc attcgatgat tctttggtca aaatcgtcac ctccgaggtg agtatctcca
     3901 tcggtggctt tcacttcaaa tacaccatct cccaattcca ataccgaaac atcatgcgtt
     3961 ccaccaccgc agtcaaacac cacaattttc atttcccggt tggttttgtc taatccgtaa
     4021 gccaaagcag aagccgtagg ttcattaata attcgacgaa catttaaccc ggcaatttct
     4081 cctgcttctt ttgttgcttt acgctgtgca tcattaaaat aagccggaac ggtaataatg
     4141 gcatcagtaa cctgagttcc caaataatct tcggcagttt gtttcatttt ttgaagaatg
     4201 atagccgaaa tttcttgcgg agagtaggta tgaccgtcaa tatctacttt cggtatgttg
     4261 ttttctcctc tcactacttt gaaaggtact ctcgctattt cattgg
//
LOCUS       DAOSIB010000120         4169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_18827_length_4169_cov_15.468887, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000120 DAOSIB010000000
VERSION     DAOSIB010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18827_length_4169_cov_15.468887"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..3964)
                     /gene="purL"
                     /locus_tag="PLD44_08335"
                     /gene_synonym="purI"
     CDS             complement(248..3964)
                     /gene="purL"
                     /locus_tag="PLD44_08335"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOF99369.1"
                     /translation="MIYFFQSSEKKIFVVQSAQLLETDISKLKWLFSGAELLDALWVE
                     GNFIGPRREMITPWSTNAVEITQNMGINGIVRIEEFREIPANIPLADAEFDPMLERSY
                     NGLNQEIFNIDKVPDPIVYIDDIAAYNEKEGLALSKEEIDYLNEVSRKIGRQLTDSEV
                     FGFSQVNSEHCRHKIFNGKFIIDGEEKQLSLFQLIKKTSEENPNKLVSAYKDNVAFIK
                     GPDIEQFAPVKANVPDFFELKKISSVLSLKAETHNFPTTVEPFNGAATGTGGEIRDRL
                     AGGKGSLPLAGTAVYMTSYPRYHEENEKERQWENAVKPRKWLYQSPEQILIKASNGAS
                     DFGNKFGQPLICGSLLTFEHEENGKKYGYDKVIMLAGGVGFANERDALKEAAEPGEKV
                     VLMGGDNYRIGMGGGAVSSVETGQYDNAIELNAVQRANPEMQKRVANVIRALVEAPEN
                     PVVSIHDHGAGGHLNCFSELVENTGGKINIASLPIGDPTLSAKEIIGNESQERMGLLM
                     KEKDIEETKKIALRERAPFYVAGETTGDMRLVFEQADGQKPLDLKLEYLFGKPPRTVI
                     TDKTLKDEFEPVKFDEEKLQEYLELVLQLESVACKDWLTNKVDRSITGKVARQQCVGE
                     IQLPLSDTGVVALDYRGTSGMATSIGHAPLAALIDPAAGSVLSVAEALTNIVWANLVN
                     GLDSVSLSANWMWPGKNEGEDARLYKAVEACSDFVCSLGINIPTGKDSLSMTQNYGKE
                     KVFAPGTVIISAAGEVADVKKTVSPVLVNDVDSAIYYIDFSFDSFKLGGSALTQVLNK
                     IGTETPTVKDAEYFKTAFDTIQELIEKNLILAGHDISAGGMIVALLEMCFPNTSGGLK
                     IQLDTLVEKSLITILFSENPGVLIQVQNAKTKVVEKILIDNGIGFARIAFPIPENRIE
                     ICKKKLSYCFSIAQLRDVWYRTSYLLDRKQSGKNCALQRYENFKNQPLTFSFPQTFNG
                     KLSQFGLSMHHAKTGAKAAIIREKGTNGDREMAFALYLAGFDVKDVHMTDLVSGRETL
                     EDVNMIVFCGGFSNSDVLGSAKGWAGGFLYNEKAKLALDNFYRRDDTLSLGICNGCQL
                     MIHLGLITPEHEQKPQMLHNDSHKFESGFVSVEIPENHSVMLETLTGSKLGVWIAHGE
                     GKFSLPKAKDDYHIAAQYVYPEYPGNPNGSDYNVAALCSSDGRHLAMMPHPERAIFPW
                     QWAYYPANRKNRDEISPWLEAFVNARKWIEKRKK"
BASE COUNT         1125 a          923 c          786 g         1335 t
ORIGIN      
        1 tcatataaaa atttgataat taataaggta ataaggttta tttttgtttt tgtgattatc
       61 gttactaagg ttttatttaa aaatatggtt cgtttcttga atataaagac tttatttaga
      121 gataactaac cttagcaaca tgtgaatatc atcttttcat caacataata agcttatttt
      181 atgaactcac gttaaaataa taattttttc gatgggggtc aaatttttat accgaacgag
      241 ttcaaattta tttctttctt ttttcaatcc attttctggc attgacaaaa gcttccagcc
      301 agggcgaaat ttcgtctctg ttttttctgt tggcgggata ataagcccat tgccagggaa
      361 agattgcccg ttccgggtgt ggcatcatgg ctaaatgtcg tccatccgaa gaacaaagag
      421 cggccacgtt ataatctgaa ccattcggat taccgggata ttcgggataa acatattgtg
      481 ctgcaatgtg gtaatcatct tttgcttttg gcaatgaaaa tttgccttct ccgtgtgcga
      541 tccaaactcc cagcttgctt ccggtcaatg tttccaacat gactgaatga ttttctggaa
      601 tttctacact tacaaaaccc gactcaaatt tatgggaatc gttgtgcagc atttgtggct
      661 tttgttcatg ttcaggcgtt atcagtccca aatgtatcat cagctggcaa ccattgcaaa
      721 taccgagact caatgtatcg tctcttcgat aaaaattatc cagcgccaat ttggctttct
      781 cgttatacag aaatccgcct gcccaacctt ttgcagagcc caacacatcg gaattggaaa
      841 atcctccgca aaatacaatc atattgacgt cttccaatgt ttcgcgcccg gaaaccaaat
      901 cggtcatgtg cacatctttg acatcaaatc cggccagata tagcgcaaaa gccatctcac
      961 gatcgccatt cgtgcctttt tcccgaatta tggcagcttt tgcaccggtt tttgcatgat
     1021 gcatggacaa gccgaactgc gaaagttttc cgttgaaagt ttggggaaaa gaaaaagtca
     1081 atggctgatt tttaaaattt tcataccgtt gcaaagcaca gttttttcca ctttgtttgc
     1141 ggtcgagcaa gtaggaagta cgataccaaa catctcgcaa ttgggcaata gaaaagcagt
     1201 aggataattt tttcttgcaa atttctattc ggttttccgg tatgggaaat gctatccgtg
     1261 caaaaccgat tccattgtcg atgagaattt tttcaactac tttggttttg gcattttgaa
     1321 cctgaatcag cacacctgga ttttccgaaa aaagaatggt tatcaacgat ttttcaacca
     1381 gcgtatccaa ctgaattttc aagcctccgc ttgtattggg gaaacacatt tccaataaag
     1441 caactatcat tccgccggca gaaatatcgt gtccggccaa aattaaattt ttttcgatca
     1501 attcctgtat ggtatcgaat gccgttttaa aatactctgc atctttgacg gtgggcgttt
     1561 cggtgccaat tttgtttaaa acctgtgtca gtgccgagcc acccaattta aagctgtcga
     1621 aagaaaaatc gatgtaataa atagccgaat cgacgtcgtt taccaataca ggcgaaacgg
     1681 tttttttaac atcggcaact tcgccggcag ccgaaataat gactgttccg ggtgcaaaaa
     1741 ctttttcttt tccgtaattt tgagtcatgg aaaggctatc cttacctgtc gggatattga
     1801 tacccaacga acagacaaaa tcactgcaag cttctacagc tttataaagt cgtgcatctt
     1861 ctccctcgtt ttttcccggc cacatccagt ttgcactcaa cgacacactg tccaatccat
     1921 tcaccaaatt tgcccaaacg atattggtca gggcttctgc cacagacaac accgaacctg
     1981 ctgccggatc aatcagcgca gccaaaggcg catgtcctat ggaagttgcc atgcccgaag
     2041 tgcctcgata atccaatgcc acaacacccg tatcgcttaa aggaagttga atttctccaa
     2101 cgcattgttg acgggcaact ttgccggtga tggaacgatc cactttgttg gtgagccaat
     2161 ctttgcaggc taccgattcc aattgtaaaa caagttccag atattcctgt aatttttctt
     2221 catcaaattt taccggttca aattcatctt tcaaagtttt gtcggtgata acggttcgcg
     2281 gtggtttacc gaaaagatat tccaatttta aatccagcgg tttttgcccg tctgcttgct
     2341 caaaaaccag acgcatatcg ccggttgttt cgccggctac ataaaacgga gcacgttcgc
     2401 gcagggctat tttttttgtt tcttcaatat ctttttcttt catcaataaa cccattcgtt
     2461 cctgactttc gttgccgata atttcttttg cacttagtgt cggatcgcct atgggaagcg
     2521 acgcaatatt gatttttcct ccggtatttt caaccaattc ggaaaagcag ttgagatgac
     2581 cgccggcgcc atgatcgtga atcgaaacga caggattttc cggtgcttcc accagagcac
     2641 gaataacatt ggcgactctt ttctgcattt cgggattggc acgctgaaca gcattcaatt
     2701 ctatagcatt gtcatattgt ccggtttcaa cagatgaaac ggctcctcca cccattccga
     2761 tgcgataatt gtctccaccc atcaaaacaa ctttttctcc cggttcggcc gcttctttca
     2821 gtgcatcccg ctcattggcg aaacctacgc ctccggccag catgattact ttgtcgtaac
     2881 cgtatttttt cccgttttct tcgtgttcaa aagtcagcaa agagccacaa ataagcggct
     2941 gaccaaattt atttccgaaa tcggaagctc cgtttgaagc tttaattaat atttgttccg
     3001 gagactgata caaccatttt ctgggtttta ccgcgttttc ccactgacgc tccttttcat
     3061 tttcttcgtg gtatctgggg taacttgtca tatataccgc cgtacctgcc aatggcaaac
     3121 ttccttttcc tcctgccagt cggtcgcgaa tttctccgcc tgttccggta gcagccccat
     3181 taaatggttc aacggtggtg ggaaaattat gtgtttcagc ttttagcgaa agaacagatg
     3241 aaattttttt caattcaaaa aaatccggaa catttgcttt gacaggtgca aactgctcga
     3301 tgtcgggccc tttgatgaaa gctacgttat ccttgtaggc cgaaaccagt ttgttgggat
     3361 tttcttctga agttttcttt attaactgaa ataaagatag ttgcttttct tctccatcaa
     3421 taataaattt tccgttgaaa atcttgtggc gacaatgttc ggaatttacc tgcgagaatc
     3481 caaaaacttc actgtccgta agttgtctgc caattttccg acttacttca ttcaaataat
     3541 caatttcttc cttgctcaat gccaaacctt ccttttcgtt gtaggctgca atatcgtcga
     3601 tgtaaacgat tggatcagga actttgtcga tgttgaaaat ttcctgattc aaaccattgt
     3661 agctccgttc gagcatggga tcaaactcag catcggcaag tggaatatta gccggaattt
     3721 ccctgaactc ttctatacga acaatgccgt tgattcccat attttgagtt atttcaacgg
     3781 catttgtact ccaaggggtt atcatttcac ggcgtggccc aataaaattt ccttctaccc
     3841 aaagggcatc cagcagctca gctccggaaa acagccattt taacttggag atatcggttt
     3901 ccagcaattg agcagactga actacaaaga tttttttttc tgaagattga aagaagtaaa
     3961 tcatattttt tgaggattag ttgttttcct tgttaagcgt aaaatattta attgtcggta
     4021 attttgagag aatatttcta aaaactcaaa tcacgatgtc taacaaagta agtcgttttt
     4081 cggcaataaa gttttttcat cagaatataa tttgtttttt aaaggtctga tggaacccac
     4141 atgtctgaat attatattca ttcgtgttt
//
LOCUS       DAOSIB010000121         4152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_18938_length_4152_cov_10.446668, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000121 DAOSIB010000000
VERSION     DAOSIB010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18938_length_4152_cov_10.446668"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..1191
                     /locus_tag="PLD44_08340"
     CDS             268..1191
                     /locus_tag="PLD44_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOF99370.1"
                     /translation="MKISNLLVILGLLFCFSFVQAKSIQKITRTYAVKDSLELKMDIY
                     TVDSTSSSLQPCLVFVFGGGFKEGKRDEPTYQPYFDYFAKKGFMVVSIDYRLGMKDQR
                     SPGVFNTKPLQNAISLAVLDLYSATKFLIEKADSLKIDKNKIIISGSSAGAITVLQAE
                     YELKNHHAITSELPHDFRFAGVISFAGGIFSTKGMPSYAESPAPTLFFHGSADKLVPY
                     KETRLFNKGMFGSRSLTNNFHKKKYSYLFYSMEGLGHEVASFPMKEFLPEIEQFIQNF
                     VFEQKPWMVEVYFTDKERKTEKSVTPADYYK"
     gene            complement(1253..2980)
                     /locus_tag="PLD44_08345"
     CDS             complement(1253..2980)
                     /locus_tag="PLD44_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99371.1"
                     /translation="MKKHQKKILIALLLFSNILFVVAQDVPEHISYSRIYDFLDELAN
                     EKLIEINSVVKPYPRTFIAQLLQQAKSKEAKLNRRQQKELYFFLENYALEQNRLPQYD
                     YPLFEQTNWQLDVFPPSVHYRDSLLRGRVMPILGMNILYNNNDKITQRWFGIDFQSMI
                     GKHFAVYASLRDVSQQGELLAESTYLTPYQGGAYKRTANGGDYSEMRGGISYAWEWGS
                     VALTKDHVQWGDNYHGSNIIAGRTPSFPMLKLSLKPAKWLEMNYFHGWLTSNVIDSTR
                     YYLSNQNEKMYRYTNKFLAANMITFIPLPQLHLSAGNAIVYAESNVQPAYLIPLAFYK
                     SMDHTLTMGTENQNSQLFLNISSRNIQHLHLFTSIFVDEFSISRIKPSSKEKNPISYK
                     LGFRLSNFPLENLSLTGEYTKNAILTYKHSIPALTWATNGFNLGNYLGDNSQEIYAAL
                     QYKPLRGLDINLSWTQAEHGNEYAYIRHGTWQGSKASVLDIISQPFMKDKIWSNKTLA
                     LHVKYELFPNTYATLNIENSYIQTFNAVSEVTFGERRMTAEEALNHYTPKFLQGKQTT
                     FTAGFSFGF"
     gene            complement(3162..>4152)
                     /locus_tag="PLD44_08350"
     CDS             complement(3162..>4152)
                     /locus_tag="PLD44_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOF99372.1"
                     /translation="LQSRFDLRKDDVRVFLNTSIENLELIPHYHYSTFIYLRKLNDTE
                     KINTFFSGINEKLPDDGNFVCCFEKKSTRKKKFLQKYPPGINYILYSLDYFFKRFIPK
                     LFFSRRIYHSLKGGKNRILSKAEVLGRFYCHGFEVIEEKKIDGLLYVWAQRKRNPTPV
                     YNRAYGPLIKLKRYGKNGKEIYVYKFRTMHPYSEYLQEYVYQRHSLQDGGKFNKDFRV
                     TTIGKFMRKYFLDELPMLINIFRGEMKLIGIRPLSQQYLNLYNDEIQQLRMKHRPGLL
                     PPFYADMPRTLDEIQESERKYLLACEKNGVLWTDIKYFFLILKNILFHHARSA"
BASE COUNT         1244 a          725 c          818 g         1365 t
ORIGIN      
        1 ataaaagtat gttgcccaca aagatattaa tttataagct aaatttaaca taaaggaaat
       61 ttttagtgag acaaatttcg tacttttgtt ctaaagtttt atgctttatt ttggtaattt
      121 tttaggggaa aagtggcaag gtcaaaaata tgctttacaa agcagcaagg attttattgc
      181 ttgttttgtg ctaatttttc tttacctttt cttttcctaa aaagttgcat tattcagttg
      241 actttacgac aaataaaatt tatacatatg aaaatcagta acttattagt aattttaggt
      301 ttattatttt gtttttcttt tgtacaagcc aaaagcattc aaaaaataac acgtacttat
      361 gctgttaagg attctttgga actgaaaatg gatatttata ctgtcgattc cacatcttct
      421 tctttgcaac catgtttggt atttgtattt ggtggagggt ttaaagaagg gaaaagagac
      481 gaaccaactt accaacctta ttttgattat tttgcaaaaa aaggttttat ggtggtttca
      541 attgactatc gattggggat gaaagaccag cgctcaccgg gggtttttaa taccaaacct
      601 ttgcaaaatg ccatttcttt agctgtttta gacttatatt ctgccacaaa atttttaata
      661 gaaaaggctg attcattgaa aattgacaaa aataagatca ttattagcgg ttcaagtgcc
      721 ggtgccatta cagttcttca ggctgagtac gaattaaaga atcatcatgc tataacatcc
      781 gaattacctc acgattttcg ttttgcaggc gtaatttcct ttgcaggggg cattttcagc
      841 acgaaaggca tgccgtctta cgctgaatca cctgcaccaa ccctgttttt tcatggtagt
      901 gccgacaaac tggtgccata taaggaaact cgacttttta acaagggcat gtttggttcc
      961 agatcgttga ccaacaattt tcataaaaag aaatattctt atctttttta cagcatggaa
     1021 ggtttaggac atgaagttgc aagttttcca atgaaagaat ttttacctga aattgagcaa
     1081 ttcattcaaa attttgtgtt tgaacaaaaa ccctggatgg ttgaagttta ttttaccgac
     1141 aaggagcgaa aaacagaaaa atcagttact ccggccgatt attacaagta atcagtttgc
     1201 cgaaaaacgt ttttcgctat ttatactttc atcattttaa gaaaactttt gttcaaaatc
     1261 caaagctaaa gccggcagta aaagtagttt gtttaccctg aagaaatttt ggcgtatagt
     1321 gattcaatgc ttcttcggca gtcattctcc tttcaccaaa agtaacttcc gaaacagcat
     1381 taaaagtttg tatatagctg ttttctatgt tcaatgttgc ataagtattg gggaaaagct
     1441 catattttac atggagtgcc agagttttat tgctccaaat tttgtctttc ataaacggtt
     1501 gactgataat gtcgagaaca cttgcttttg agccttgcca ggttccgtgt cgaatatagg
     1561 catattcatt tccgtgttcg gcttgtgtcc acgacaggtt tatgtccagc ccgcgcaaag
     1621 gtttgtattg caaggcggca taaatttcct gcgaattgtc gcccaagtaa tttcccagat
     1681 tgaaaccatt ggttgcccat gtcagagcag gaatagaatg tttgtaagta aggatggcat
     1741 tttttgtata ttcacccgtc aacgaaagat tttccagcgg gaaattgcta aggcgaaatc
     1801 caagtttata ggaaatagga tttttttcct tgctggaggg tttaatgcgg gaaatggaga
     1861 attcgtccac aaaaatggaa gtaaacaagt gcaaatgttg tatgtttctt gaactgatat
     1921 taagaaacag ttgcgaattc tgattttcgg tacccatagt cagcgtatga tccatcgatt
     1981 tgtaaaaagc taaaggaatc aaataagcgg gctgtacatt gctttcggca tacacaatgg
     2041 catttcccgc cgacagatgc aattggggca ggggaataaa tgttatcatg ttggcagcca
     2101 gaaatttatt ggtgtaacga tacatttttt cattttgatt ggaaagataa taacgtgtgc
     2161 tgtcgattac attggaagtc agccacccat gaaaataatt catttccagc cattttgccg
     2221 gtttcaggct cagcttcagc atgggaaacg aaggcgtgcg acccgcaata atgttggaac
     2281 cgtgatagtt gtcgccccat tgcacatggt cttttgtcag tgcaacactt ccccattccc
     2341 aagcatacga aataccgcca cgcatttcgc tgtaatcgcc accattggca gtacgtttgt
     2401 aggctccgcc ctgatacggt gtcaggtaag ttgattcggc aagcaattct ccctgttgcg
     2461 aaacatcccg gagggaagca tagacggcaa aatgttttcc tatcatcgat tgaaaatcaa
     2521 ttccaaacca gcgttgcgta attttgtcgt tgttgttgta gagaatattc attcccaaaa
     2581 tgggcatcac acgtccacgt aacagtgaat cgcggtagtg aacggaagga gggaaaacat
     2641 ccaactgcca gttggtttgt tcgaaaagcg gataatcgta ttgcgggagg cgattttgtt
     2701 ccaaagcata attttccaga aaaaaataca attctttctg ttgacggcgg ttgagttttg
     2761 cttcctttga ttttgcctgt tgcaacaatt gggctataaa agttcttgga tagggcttaa
     2821 caaccgagtt gatttcaata agtttttcgt tggcgagctc atccagaaaa tcgtaaatgc
     2881 gggagtagga aatatgttcg ggaacatctt gagcaacaac aaaaaggata ttggaaaaga
     2941 gcagcagtgc gataagtatt tttttttgat gttttttcat gttgtagcca aatttttaag
     3001 atttttcaaa attagttgaa aataaaggca atatgttgtt attaaaaaat tttttcgcta
     3061 aagaagtctt gaaatttttt tgaaaaaagt ttgatttaac ctgttgctta ttttggttcc
     3121 ttatactttt gctttttata agttcttgga ttcttgaatt ttcaggcact tcgtgcatga
     3181 tgaaacagaa tattcttcaa aatgagaaag aaatatttta tatcggtcca taaaacgcca
     3241 tttttttcgc aggccagtaa atattttcgt tccgattctt gaatttcatc caacgtgcga
     3301 ggcatatcgg catagaaagg gggtagcaat ccgggtcggt gtttcatgcg aagttgttga
     3361 atttcgtcat tgtacaggtt aaggtattgc tggctaagtg ggcgaatgcc aatcaatttc
     3421 atttcacccc tgaaaatatt gatcagcatg ggcaattcgt ccagaaagta tttgcgcatg
     3481 aattttccga tggtggttac ccgaaaatct ttgttgaatt ttcctccatc ttgtaaagaa
     3541 tgccgctgat aaacatattc ctgaagatat tccgaatagg gatgcatggt gcgaaattta
     3601 taaacataga tttcctttcc gtttttgcca tagcgtttca atttaatcaa tgggccatag
     3661 gcacgattgt aaacgggtgt cggatttcgt ttcctttgcg cccatacgta cagaagtcca
     3721 tctatttttt tctcttcaat cacctcaaaa ccgtgacaat aaaatctccc caatacttct
     3781 gctttggaaa ggatacggtt ttttccacct ttcagcgaat gataaatgcg cctcgaaaag
     3841 aaaagttttg gaataaaccg cttgaaaaaa taatctaaag aatataaaat ataattgata
     3901 cccggaggat atttttgaag gaatttcttt tttctggtgc ttttcttttc aaaacaacaa
     3961 acaaaatttc catcgtctgg caatttttcg ttgatacccg aaaaaaaagt gttgattttt
     4021 tccgtatcgt tcaacttacg caggtagata aaagttgaat aatgataatg aggaatcagt
     4081 tcaagatttt caatgcttgt atttaagaaa accctcacat catcttttct caagtcgaaa
     4141 cggctttgca aa
//
LOCUS       DAOSIB010000122         4038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_19617_length_4038_cov_10.882501, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000122 DAOSIB010000000
VERSION     DAOSIB010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19617_length_4038_cov_10.882501"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /locus_tag="PLD44_08355"
     CDS             complement(<1..1270)
                     /locus_tag="PLD44_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOF99373.1"
                     /translation="MNKEWIELSKTLNKVIDYRGKTPKKLGFEWSETGYRALSALNVK
                     TNGLENLDSIKYASEELYKKWMKEEIQRGDILLTSEAPAGQVMVWDSDEKIVLSQRLF
                     ALRLNSEYDNYFIKYFLQSDIGQKEINKNTTGSTVFGISAKMFDLIKVPKIKKERQVL
                     IGKILKSLDAKIKLNNRINRELEAMAKTLYDYWFVQFDFPNADGKPYKSSGGKMVYNA
                     ELKREIPEGWEVKKLGELVYQVTDSISPDEYPELPYLPIDKLPMKQLYYYEYESRSEA
                     NSSLIRFKENDILLGAMRVYFHRVCHAISDGISRSTIIVLRPFKENTKNFALFALNKN
                     EAIEFATNNSTGTSIPYAKWNDSLENYLIGVPKTDYILILFNKIVNPILEKFKVQNKQ
                     TQQLTELRDWLLPMLMNGQVTVRQAHGNAVK"
     gene            complement(1263..2888)
                     /locus_tag="PLD44_08360"
     CDS             complement(1263..2888)
                     /locus_tag="PLD44_08360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009436644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOF99374.1"
                     /translation="MTDIDFKEKTKTLIDSLKSISANYGLGNDGNEFKIITQVFLYKF
                     MNDKFAFELKKLDPKLKNADNWEETVKSYSDDDYEMLLLQLGADTAKLKPQHFISELF
                     GRQNESDFAKLFDDTLMDIAITNNDIFSVKTDGGAKVVLFDRLTEYISDGSKRDAFAK
                     AIINKLVGVSFEHIFHQKFDFYATIFEYLIKDYNTNSGGKYAEYFTPHAVAKIMAAIL
                     VNEPVQDVTVYDPSAGSGTLLMNIAHAIGEDKCTIYSQDISQKSSSLLRLNLILNNLV
                     HSIQNIIQGNTILHPYHKDKEGNLQKFDYIVSNPPFKLDFSDYRDDLDSKENNERFFA
                     GIPNVPKQAKDKMAIYLLFLQHIMHTLSKKGKAAIVVPTGFITAQSGIDKKIREKMVE
                     SKMLAGVVSMPSNIFATTGTNVSIVFLDKTNKNEVVLIDASNLGETVKEGKNQKTVLT
                     PAEEQQIIDTFNQRKAVDDFSVVVSYDEIKQKNYSLSAGQYFEVKIEYVDITAEEFAA
                     KMKGFEHNLNQLFAESRELEMEIQNNLKGFGYE"
     gene            complement(2885..>4038)
                     /locus_tag="PLD44_08365"
     CDS             complement(2885..>4038)
                     /locus_tag="PLD44_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99375.1"
                     /translation="MLLTGFDAKRLKKLYIGRVIKRHNLLQTLTRVNRTYKNFKYGYV
                     VDFADIRAEFDATNKAYFDELQAELGDEMEFYSDLFKSKEEIEKEIAEIKDILFYFDT
                     TNAELFSQQITQIQDREKVLLIKKALGNAKSLYNLIRLFGHFDLLEKIDFKKLAQLYR
                     ETDNHLALLNTQEALKNNIDITNLLNVALEDVLFHFTKISEEELILADELKDTLRKTR
                     ETLASNFDKKDPEFVSLYEELKRLFDKKNLDEITQDEMKQNIGALTKIYEKIKELNRQ
                     NNLLKDKYDNDPKYARIHKRLIEKNTLSQKESVIFEALKSVKQDADLQVLQNTRLLDN
                     ESYFTTQMMRLVIDQFKNKNNINLNAESSKYINNLVVNEYINEFYGRAV"
BASE COUNT         1075 a          785 c          717 g         1461 t
ORIGIN      
        1 cttttactgc gttgccgtga gcttgtcgaa cggttacctg cccattcatt aacataggca
       61 acaaccaatc tcgaagttct gttagttgtt gggtttgttt attttgaact ttgaattttt
      121 ccaaaatagg attaactatt ttattgaata atatcaaaat ataatctgtt ttcggaactc
      181 ctattaaata attttcgagg ctatcattcc attttgcata tggtattgat gtacctgttg
      241 aattattagt tgcaaattca attgcttcat ttttatttaa tgcaaaaaga gcaaaattct
      301 tagtgttttc tttaaatggt cttaaaacga ttattgtact cctagaaatc ccatcagata
      361 ttgcatggca aacacgatga aaataaacac gcatagcccc taataaaata tcattttctt
      421 taaaccttat taaactagaa ttggcttcag aacggctctc gtattcataa taatatagct
      481 gtttcatcgg tagcttatct attggtaaat atggtaattc aggatactca tctggtgaaa
      541 tactgtctgt aacttgataa actaactctc ccagcttttt cacctcccac ccctccggaa
      601 tctccctttt caactcggca ttgtacacca tttttccacc tgaggatttg tagggcttgc
      661 catctgcatt gggaaaatcg aactgcacaa accaataatc gtacagcgtt tttgccatgg
      721 cttccaactc tcggttgatg cggttgttga gtttgatttt ggcgtctagg gattttagaa
      781 ttttacctat taaaacttgt ctttcttttt taatttttgg cacttttatc aggtcaaaca
      841 ttttagctga aatgccaaaa acagtgctcc cggtagtatt cttgtttatt tctttttgac
      901 caatgtctga ttgcaggaag tattttatga agtagttgtc gtattcgcta tttaaacgaa
      961 gtgcaaaaag tcgttgactt aaaacaattt tttcatcact atcccaaacc ataacctgac
     1021 cagctggagc ttctgaagtt agaaggatat cacctctttg tatttcttcc ttcatccatt
     1081 ttttgtaaag ctcctcacta gcatatttta ttgaatcgag gttttccaaa ccattagttt
     1141 ttacattaag tgctgagagt gctctatacc cagtttctga ccattcaaag ccgagttttt
     1201 ttggtgtttt acccctgtag tcaattactt tatttagggt tttagatagt tcaatccatt
     1261 ctttattcat acccaaaccc ctttaaatta ttctgaattt ccatttccaa ttccctgctt
     1321 tcggcaaaga gttggtttag gttgtgctca aagcctttca tcttggcggc aaattcttct
     1381 gcggtaatgt ctacatattc aatctttact tcaaaatatt gcccggcact cagggagtag
     1441 tttttttgct taatctcgtc atagcttacc actaccgaga aatcatccac cgctttccgt
     1501 tggttaaacg tgtcaatgat ttgctgttct tcggctgggg tgagaacggt tttttggttt
     1561 ttgccttctt ttacggtttc gcccaggttg gaggcatcta tcagcaccac ttcgtttttg
     1621 ttggtcttgt ctaaaaatac aatgcttaca ttcgtacccg tggtggcgaa aatattgctc
     1681 ggcatactca ccacacctgc cagcattttg ctctctacca ttttttcacg aatcttttta
     1741 tcaatgccgc tctgtgcggt tataaaaccc gtaggtacaa caatggcagc ttttcctttt
     1801 ttgctgagcg tgtgcataat gtgctgcaaa aagagaaggt aaattgccat tttgtctttg
     1861 gcttgcttgg gtacattggg tatgcctgca aaaaagcgtt cgttgttttc cttgctgtcc
     1921 aaatcgtctc ggtaatcaga aaaatcgagc ttaaaaggtg gattggaaac gatataatcg
     1981 aatttctgca agttgccttc tttgtccttg tggtaagggt gcaagatggt attaccctga
     2041 atgatgtttt gaatggaatg taccagatta ttcaaaatca aattcaaacg caaaaggctc
     2101 gatgattttt ggctgatatc ctgcgaataa atggtgcatt tgtcctcgcc aatagcgtgg
     2161 gcaatgttca tcaataatgt acccgaacca gccgaagggt cgtaaacagt tacatcttgt
     2221 acaggctcgt ttaccaaaat ggcagccata atttttgcta cggcatgagg tgtaaaatat
     2281 tcggcatact tgccaccgct attggtgttg tagtccttta tcaggtattc aaaaatggta
     2341 gcgtaaaaat cgaatttctg gtggaaaata tgctcaaagc ttaccccaac caatttgttt
     2401 atgatggctt ttgcaaatgc atcacgctta ctaccatcgc taatgtattc agtaaggcgg
     2461 tcgaacaaca ccacttttgc cccgccatct gttttcaccg agaaaatgtc gttattggta
     2521 atagcaatgt ccatcaacgt atcgtcaaac aatttggcaa aatcgctttc gttttgtctg
     2581 ccaaaaagtt ctgaaataaa gtgctgtggt tttagtttgg cggtatcggc acccaattgc
     2641 agcagcaaca tttcgtaatc atcatccgaa taggatttta cggtttcttc ccaattgtcg
     2701 gcattcttca attttgggtc aagtttcttg agttcaaagg cgaacttgtc gttcataaat
     2761 ttatacagga atacctgcgt aatgattttg aactcgttgc catcattgcc caagccataa
     2821 tttgcactaa tgcttttaag gctgtcaata agtgttttgg tcttttcttt aaaatctata
     2881 tctgtcatac tgctcttccg tagaattcgt taatgtattc gtttaccacc aagttgttaa
     2941 tgtacttcga tgattcagcg ttcaaattta tattgttttt gtttttgaat tggtcaataa
     3001 ccaatcgcat catttgtgtt gtgaaatagc tttcattatc taacaaccga gtgttttgta
     3061 gcacttgcaa atcagcatct tgctttacac tttttaacgc ttcaaaaatc acactttcct
     3121 tttgcgataa agtgtttttt tcaatcaagc gtttatggat tcgggcatat ttcgggtcgt
     3181 tgtcgtattt gtctttcagt aaattgtttt ggcggttgag ttcttttatt ttctcgtaaa
     3241 tctttgtcaa tgcaccaatg ttttgtttca tttcgtcctg tgtaatttcg tcaaggtttt
     3301 tcttgtcgaa taagcgtttc agttcttcat ataacgaaac aaattcaggg tcttttttat
     3361 caaaattact tgccaaagtt tcacgagttt tccgcaaagt gtcttttaat tcatcggcta
     3421 aaatcagttc ttcttccgaa attttggtaa agtgaaacag cacatcttcc aaagcaacat
     3481 taagtaaatt ggtaatgtca atgttgtttt ttaaagcctc ctgcgtattg agtagagcta
     3541 aatgattatc ggtttctcgg taaagttgag ccaacttttt gaaatcaatt ttttcgagca
     3601 aatcaaaatg accgaataaa cggattaggt tgtataaact cttggcattg cccaaagcct
     3661 ttttaatgag caatactttt tcacggtctt gaatttgggt tatttgttga gaaaatagtt
     3721 ctgcatttgt cgtatcgaaa taaaataaaa tatcctttat ttctgcaatt tccttttcaa
     3781 tctcttcttt cgatttaaac aagtcggagt aaaactccat ttcgtcgccc aattcagctt
     3841 gcaattcatc aaaataggct ttattggtgg cgtcaaattc tgcccgtatg tcggcaaaat
     3901 caaccacata gccgtatttg aaattcttgt aagttcggtt aactcgtgta agtgtttgta
     3961 aaagattgtg cctttttata actcgcccga tgtataattt ctttaatcgt ttggcatcaa
     4021 aaccagttaa aagcatgt
//
LOCUS       DAOSIB010000123         3968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_20072_length_3968_cov_9.896754, whole genome shotgun sequence.
ACCESSION   DAOSIB010000123 DAOSIB010000000
VERSION     DAOSIB010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20072_length_3968_cov_9.896754"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1117
                     /locus_tag="PLD44_08370"
     CDS             <1..>1117
                     /locus_tag="PLD44_08370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOF99376.1"
                     /translation="LPEAATIFTTHATTVGRSIASNNKPLYDYLQEYNGDQMAAELNV
                     VSKHSAEKTAAHLSDCFTTVSDITAAECRQLLEIKPAVVTPNGFEDDFVPKADTFIQK
                     RQEARTCLRKVAETLLGYSLQENALFVGTAGRYEYKNKGIDVFIESMRRLIECPHLNR
                     EIVVFIMVPAHVMGPRTDLLAALNHPNLKLHSWNLFTTHELYDYQNDHIMSAIRWFHF
                     TNQSSQKVKMIFVPSYLNGSDGIFNKKYYDLLIGLDVTIFPSYYEPWGYTPLESIAFS
                     VPTITSDLSGFGRWVAKQPQDITEGVGVVHRSDYNQYDATVKIAQMICQFSSLEQSQV
                     ENIRSIAASISLKALWENFIQYYYEAYNIALTQKNLRL"
     assembly_gap    1118..1217
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1402..3966
                     /gene="glgP"
                     /locus_tag="PLD44_08375"
     CDS             1402..3966
                     /gene="glgP"
                     /locus_tag="PLD44_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOF99377.1"
                     /translation="MSLKLNQVNEPRWKEINVKSFLPDGLKRLEEIAQNIFWVWRSDA
                     RHLFRELDKDAWAESGYNPVQLLNMLSYERLVEVEKDAHFMSNLDRIYNEFQAYLHQP
                     FAPDKPSIAYFSMEYGLTEVLKIYSGGLGILAGDYLKEASDCGVDLTAVGLLYRYGYF
                     TQSLSVDGDQIEIYDKQNFNTLPIVQVRDENGQAVVIEVPYPGRTVYANLWKVSVGRI
                     NLFLLDTDNEMNSEADRSITFELYGGDWENRLKQEIMLGIGGILTLKKLGIEKDVYHC
                     NEGHAALINLQRLADLVTEKKLSFHEALEIVRSSSLYTVHTPVPAGHDRFDEALFGKY
                     MKDYAALLNISWDDLMNLGRENPDDPNDKFSMSVFACNTCQEVNGVSWLHGKVSQEMF
                     QPIWKGYYPEELHVSYVTNGVHLPTWTTSEWKGVYEKYLGKDFYDDQSNMKLWQAIYE
                     VPDQVIWETRVKMKNKLIDYIRLQFQENWLKSQGDPSRIMSVLESMNPNALIIGFGRR
                     FATYKRAYLLFTDLKRLSKIVNDPSKPVQFIFSGKAHPADQGGKALIKRIVEVSRMPE
                     FLGKIIFLENYDMHLAKRLISGVDIWLNTPTRPLEASGTSGQKAQMNGVLNFSVLDGW
                     WLEGYVKGAGWAITEKRTYDNDAYQDQLDAATIYSMLENEIIPLYFAKNSKGISPEWI
                     QYIKDSIAQITPRFTTKRMIDNYVEQFYTKLSNRHALLKDNNFARAKEIAAWKQKIAK
                     GWDKIEVVSMDIPEGLLKNPQVSEVYHMNIVIDIKDINDKGIGIELVITKPDKDNKFY
                     LHDVEEFKLVKANGSLLYFSLEYQLNRAGLFQFGFRMFPKNEDLPHRQDFCYVKWI"
BASE COUNT         1151 a          720 c          836 g         1161 t
ORIGIN      
        1 atttgcctga agctgcaacc atttttacta ctcatgccac tactgtggga cgttccatag
       61 ccagcaataa caaaccgttg tatgattatc tgcaagagta caatggggat caaatggctg
      121 ccgaattgaa tgttgtttcc aaacattcgg ccgaaaaaac tgccgctcat ctttccgatt
      181 gcttcacaac tgtaagcgac attactgccg ctgaatgccg acaattgctg gaaattaaac
      241 ccgctgttgt tacaccaaac ggttttgaag atgattttgt gcccaaagcc gatactttta
      301 ttcaaaagcg acaggaagca cgaacttgtt tacgcaaagt cgctgaaact ttgttgggtt
      361 attctttaca ggaaaatgct ctttttgtgg gtactgccgg acgttacgaa tacaagaaca
      421 aaggtattga tgtgtttatc gaatcgatgc ggaggttgat tgaatgtcct catttgaaca
      481 gagaaattgt ggttttcatt atggtacccg cccatgtgat gggccccaga accgatttac
      541 ttgcagcttt aaatcatcca aatctcaagt tgcattcatg gaatttgttt accacgcacg
      601 aactttacga ttatcagaat gatcatatca tgtcggctat tcggtggttc cattttacca
      661 atcaatcgtc gcaaaaggtt aagatgattt ttgtaccctc ttatttgaat ggttccgacg
      721 gcatattcaa taaaaaatat tacgatctgc tcattgggct cgatgttacc atatttccgt
      781 cgtattacga accgtggggc tatacacctc tcgaaagcat tgctttttcc gtaccaacta
      841 ttaccagcga tctgtcgggt tttggccgct gggtggcaaa acaacctcag gatattaccg
      901 aaggtgtggg agtagtacat cgttccgact acaatcagta tgatgcaact gtgaaaattg
      961 cacaaatgat ttgtcagttt tcgagtttgg agcaatctca agtagaaaat atacgctcaa
     1021 tagcggcttc aatttccctg aaagccttat gggaaaactt catacaatat tattatgaag
     1081 catacaatat agcccttact caaaaaaatc tccgactnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnntaa ttaattaatt gaaaaagaat atattaacaa aaatttttta
     1261 ataaattgca atcgtttgca tttgttttta ctccttttat gatttgtttt atcttagctt
     1321 tttgaaactt tgcttaactt tgcctacaga gttttaggta taaaattttt tttgaatttc
     1381 actaacttaa agtaaggaaa catgtctttg aaattaaatc aggtaaatga accgcgctgg
     1441 aaagaaataa atgtcaagtc ttttttgccc gatggcttga aaagattgga agaaatagcg
     1501 caaaatatat tttgggtatg gagaagtgat gcccgacacc tttttcgtga attggacaaa
     1561 gatgcttggg cggaatcagg ctataatccc gtgcaattgc tgaacatgct cagttatgag
     1621 cgacttgtag aagtggagaa ggatgcccat ttcatgtcca acttagacag gatttacaac
     1681 gaatttcagg cttatctgca tcaacctttt gcacccgata aaccttcgat tgcctacttc
     1741 agtatggaat acgggctgac ggaagtttta aaaatctatt ccggagggct tggaattttg
     1801 gcaggcgatt atttgaagga agccagtgat tgtggcgttg atttgacggc tgtcggatta
     1861 ctttatcgtt acggatattt tactcagtcg ctttcggtcg acggtgatca aattgagatt
     1921 tatgacaaac aaaacttcaa tactttgcct attgtgcagg ttcgtgatga aaatggacaa
     1981 gcggttgtta tagaagttcc ctacccggga cgcaccgtgt atgccaattt gtggaaagta
     2041 agcgttggac gtattaatct tttccttttg gataccgaca atgaaatgaa cagtgaagct
     2101 gacagaagta ttactttcga gttgtatggc ggcgattggg aaaatcgtct gaaacaggaa
     2161 atcatgctgg gaattggtgg aattctgaca ctgaaaaaac taggcatcga aaaggatgtc
     2221 tatcattgca atgaaggcca tgcagccctt atcaatcttc agcgtctggc ggatttagta
     2281 accgagaaaa aactttcttt ccacgaggct ttggaaattg tgcggagcag ttccctttat
     2341 actgtacata cacctgttcc tgcgggacac gaccgctttg atgaagcttt gtttggaaaa
     2401 tacatgaaag attatgctgc cttgctgaat atttcgtggg acgacttgat gaacttgggg
     2461 cgtgaaaatc cagatgatcc caatgacaaa ttcagtatga gtgtttttgc ctgcaatacc
     2521 tgtcaggaag ttaatggtgt aagttggttg catggcaagg tttcacaaga aatgtttcag
     2581 ccaatatgga agggttatta tcctgaagag ctgcatgtga gctatgttac caatggtgtt
     2641 catttaccca catggaccac ttcagagtgg aaaggagttt acgaaaaata tctcggaaaa
     2701 gatttttatg acgatcagtc gaacatgaaa ctctggcagg ctatttacga agtgcccgat
     2761 caagttatct gggagacgcg cgtcaaaatg aaaaacaaat tgatcgatta tattcgtttg
     2821 caatttcagg agaactggct gaagtctcag ggcgaccctt cccgtatcat gtcggttctg
     2881 gaatcgatga atcccaatgc tttgatcatc ggttttggtc gaagatttgc aacttacaag
     2941 cgcgcttacc ttttatttac cgatttgaag cgtttatcga aaattgtaaa tgacccgtca
     3001 aaacctgtgc agtttatttt ttccggtaag gcacatcctg ccgatcaggg aggaaaagcg
     3061 cttattaaac gcatagtgga ggtttctaga atgcccgaat ttttaggcaa aatcattttc
     3121 ctcgaaaatt atgatatgca tttggccaaa cgattgattt ccggcgtcga tatttggttg
     3181 aataccccta cgcgtccttt ggaagcttcg ggaacttctg gacaaaaggc acaaatgaat
     3241 ggcgtattga atttttcggt tctcgacgga tggtggttgg aaggttacgt gaaaggagcc
     3301 ggttgggcta ttaccgaaaa acgaacttac gataacgatg cgtatcagga tcagcttgat
     3361 gctgcaacca tctacagcat gctcgaaaat gaaattattc cattgtattt tgccaaaaat
     3421 tccaaaggaa tttcgcccga atggattcaa tacatcaaag attccattgc acaaattaca
     3481 ccacgtttta caaccaagcg catgattgac aattatgtag agcagtttta caccaaacta
     3541 agcaatcgtc atgcattatt aaaagataat aattttgcgc gtgccaaaga aattgctgca
     3601 tggaaacaga aaattgccaa agggtgggac aaaattgaag ttgtgagcat ggatattcct
     3661 gaaggtttgt tgaaaaatcc tcaagttagt gaggtttatc acatgaatat tgtcattgat
     3721 atcaaagaca ttaatgacaa gggtatcgga atagaactgg tcattaccaa acccgataag
     3781 gacaataaat tctatcttca tgatgtggaa gagtttaagc tggtgaaagc gaatggttca
     3841 ctattgtatt tcagtctcga atatcagctc aatagagcag gattgttcca attcggattc
     3901 cggatgttcc ccaaaaatga agatttgcct catcgtcagg atttctgtta tgtaaaatgg
     3961 atataacc
//
LOCUS       DAOSIB010000124         3934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_20288_length_3934_cov_11.911060, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000124 DAOSIB010000000
VERSION     DAOSIB010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20288_length_3934_cov_11.911060"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..697)
                     /locus_tag="PLD44_08380"
     CDS             complement(131..697)
                     /locus_tag="PLD44_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99378.1"
                     /translation="MSEENLNNTRSDEQSPVSSQPTNKKGFFRTFISRHTGVFVLLCL
                     LIAVVIFAFIRIKLLERSFNKQQEALRVRYENKVDSLLQADRLTTAKVFSWAVRGELI
                     RDNRDQINQYFMNFVKESGVKKIKLIDPVSAKAIISTDKKDEGLVFADPTFFTEEIAV
                     RHLDSVTQIISPVMGLNKKIGILVIEYE"
     gene            complement(773..1930)
                     /locus_tag="PLD44_08385"
     CDS             complement(773..1930)
                     /locus_tag="PLD44_08385"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOF99379.1"
                     /translation="MNTNNEKWHGSPKKGGNSYDSREGRHFEKGGNNSPFSKPGRNQK
                     VYSSRNTSNSNYDNRSTGGKPYYKNENKKPYRRPFTSDENSEGENRKLKLVKPGKFVE
                     TRFDDRQPNVTFRKRKNKTEGEFNPNDKYSRKKQLEYQKNNVDYSKPIRLNKFLANAG
                     ICSRREADEFIQSGVISVNGEVVTQLGAKIMMTDTVMFKNQPVQSERKVYLLLNKPKD
                     CLTTSDDPNNRKTVLDLVKNACSERIYPVGRLDRNTTGVLLLTNDGDLAAKLTHPKYN
                     KKKIYHAVLDKNLETVDYESIIQGVDLGDEHVSVDALEFVKAGNRREIGIEIHSGQNR
                     VVRRIFEKFGYKVVYLDRVYFAGLTKKNVPRGKYRFLTQREINMLKMGAYE"
     gene            complement(2145..>3934)
                     /locus_tag="PLD44_08390"
     CDS             complement(2145..>3934)
                     /locus_tag="PLD44_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOF99380.1"
                     /translation="VRRTLEAVYENYRGDRQSEEFRNFITYLKQVWMGNGIHHHYSQD
                     KFVPKFSAAFFAQAVKSIDAEVLPLEKGETPDDLIRKLTPVIFDPTVFPKRTNQADGV
                     DLLLTSAVNFYEGVTQAEAEEFYDRMRNPNDSTPISYGLNSKLMKENGVLVEKTYKLG
                     GMYGKAIEQIVYWLNKALSVAENDKQKDVIHSLIKFYETGDLKTYDEYCIKWVQDTAS
                     LVDFVNGFTETYADPLGLKGSWESIVNFKNLASTKRTETISENAQWFENNSPIDNRFK
                     KENVKGITAKVVTAAILAGDCYPSTPIGINLPNSDWIRHDYGSKSVTIENITEAYDRA
                     SLGNGFNEEFMWSDVERERAKKYGSLTNNLHTDLHECLGHGSGKLLPGVDPNGLKAYA
                     STIEEARADLFGLYYVADSKMLELGLLPDHEAYKAEYYKYIMNGLLTQLTRVQTGKNI
                     EEAHMRNRQLIARWVYEKGLPENVIELKKRDGKTYVVVNDYEKLRILFGKLLAEIQRI
                     KSTGDYNGAKTLVENYGVKVDPVIHKEILERYEKLNLAPYRGFVNPVYKAIKNAGGEI
                     RDVKISYDENYVEQQLRYSRQYSNLPTFN"
BASE COUNT         1142 a          837 c          672 g         1283 t
ORIGIN      
        1 ttttaaaaag acgaaatgat ttccccgctt ctttttaaaa ttgaaacaaa aaaaattcag
       61 aacaaaaata aaaaggctga tcaaaaaaaa ttttaaacag tcttttgtac atgtcaaaaa
      121 aaaaagaaat ttattcgtat tctattacca aaatgccaat ttttttattc agtcccataa
      181 ccggagaaat aatttgagtt acggaatcaa gatggcgaac tgcaatttct tcagtgaaga
      241 aagtgggatc ggcaaaaacc aaaccttcat cttttttatc ggtagagata attgcttttg
      301 cagaaacagg gtcaatgagt ttaattttct ttacgccgga ttccttgaca aaattcataa
      361 aatactgatt tatctgatct cggttatcac gtatcagctc acccctcact gcccacgaaa
      421 aaactttggc cgttgtcagc ctgtccgctt gaagaaggct gtctacttta ttttcgtatc
      481 gaacccgcaa agcctcctgt tgtttgttga aactgcgttc caataattta atacggataa
      541 aggcaaaaat aactacagct atcagcaaac acaacaatac gaaaactccc gtatgacgtg
      601 aaataaatgt tcggaaaaaa ccttttttat ttgtaggctg cgacgaaacg ggactttgct
      661 cgtcagaacg agtattgttc aaattttctt cactcatatc acatttggtt ttaaatgatt
      721 gtagtataaa attttttaaa aaaattcata agaaaaaccc ataagaactt ttttattcgt
      781 aagctcccat tttcagcata ttgatttcgc gctgagtcag aaagcgatat tttcctctgg
      841 gtacattctt tttggtcaac ccggcgaaat aaacacggtc aagatatacg actttataac
      901 caaatttttc aaaaattcgc cttactaccc tgttttgtcc tgaatgaatt tctattccta
      961 tctctcttcg attgccggct tttacaaatt cgagtgcatc gaccgacaca tgttcatctc
     1021 ccaaatctac accttgtata atagattcgt aatcgactgt ctcaagattt ttgtcgagaa
     1081 cggcatggta aattttcttt ttgttgtatt tcgggtgggt caatttggct gccaaatcac
     1141 catcgttagt gagcagtaaa acaccagtgg tatttctgtc gagccttccg acgggataaa
     1201 tcctttcact gcaagcattt tttaccaaat cgagcactgt tttgcggtta ttgggatcat
     1261 cagaagttgt caggcaatct ttcggcttat tgagcaatag gtacactttt ctctccgact
     1321 gcacgggctg gtttttaaac atcaccgtgt ctgtcatcat aatttttgca ccgagctgag
     1381 taaccacttc accattaacg gaaatgacac ccgactgaat aaattcatcg gcttcgcgtc
     1441 gggaacaaat accggcattg gccaaaaatt tgttcaaccg aataggtttt gaataatcga
     1501 cattattttt ttgatactcg agttgttttt tacgactgta tttatcattg ggattaaact
     1561 cgccttcggt tttatttttt cgtttacgaa aagttacatt gggttgtcgg tcatcaaaac
     1621 gtgtttcgac aaacttaccg ggttttacca gcttcaattt gcgattttca ccttccgaat
     1681 tttcatcact tgtaaacggt cgtctgtatg gtttcttgtt ttcgttctta taatagggtt
     1741 ttcctccggt agaacgattg tcataattgg aattggaggt atttcgagaa gaataaacct
     1801 tttggttcct tcccggttta ctgaaaggcg aattattgcc gcctttttca aaatgtctgc
     1861 cctcacggct gtcataagag ttcccccctt tttttggact cccatgccat ttttcgttgt
     1921 tcgtattcat ttcacaatgt tattcatttt taggctgcaa agttcggaaa aattccttaa
     1981 atatcaacag ttttttagcc aaatttaggc attatccaca acaattgtta cacttacttc
     2041 ctgtcatggc gacaataacc aacaaagtaa gttcctttac gaaaaaaatt cctttgaaat
     2101 tttattaaac tattattcaa caaacaatta caaaaaaatg cttttcaatt gaatgtcggt
     2161 aaattggaat attgtcttga atacctcaat tgttgctcca cataattttc gtcataagaa
     2221 attttcacat ccctgatttc cccaccggca ttttttatag ccttataaac aggattgaca
     2281 aaaccacgat aaggtgcaag atttaatttt tcgtagcgtt ccaaaatttc cttgtgaata
     2341 acaggatcaa cttttactcc gtaattttct accaaagttt ttgcaccatt gtaatcgccg
     2401 gtggacttaa tgcgctgaat ttcggccaga agttttccaa aaagaattct cagtttttca
     2461 taatcattga ccacaacgta agtttttcca tctctttttt taagttcaat cacattttcg
     2521 ggcaaccctt tttcgtacac ccagcgagca ataagttgac gattgcgcat gtgagcctct
     2581 tcaatatttt tccctgtctg aacgcgcgtc agctgagtga gaaggccatt catgatatac
     2641 ttataatact cggctttata ggcttcatgg tcgggcaaca gtccgagttc gagcattttt
     2701 gaatcagcca cataatacaa tccgaataaa tcggcacgtg cctcttcaat ggtcgatgca
     2761 taagctttca acccattggg gtcgacgccc ggaagcaatt tcccggaacc atgccccaga
     2821 cattcatgca aatcggtgtg caaattattg gtcaacgaac cgtacttttt agctctttcg
     2881 cgttccacgt cgctccacat aaactcttcg ttgaatccgt ttcccaaaga agcccggtcg
     2941 taagcttccg taatgttttc aatagtaacc gattttgaac cataatcgtg cctgatccaa
     3001 tcggaattgg gaagattaat gccaatcgga gtggacggat agcagtcgcc tgccaaaatg
     3061 gctgcagtaa ctacttttgc tgtgattccc ttcacatttt cctttttaaa acgattatcg
     3121 atgggcgaat tattttcaaa ccactgcgca ttttcgctga tggtttcagt tcttttggtg
     3181 gaagccaaat ttttaaaatt cacaatagat tcccaactgc ctttcaatcc aagcggatcg
     3241 gcataggttt cggtgaaacc gtttacaaaa tctaccaatg acgcagtatc ctgcacccat
     3301 tttatacaat attcgtcata ggttttcaaa tctccggttt cataaaattt tatcaatgaa
     3361 tgaatgacgt ccttttgttt atcattttcg gccacagaaa gagctttatt gagccagtaa
     3421 acaatttgct caatggcttt accatacatt cctcccaact tatatgtttt ttctaccaaa
     3481 acaccgtttt ctttcatcag tttactgttt agtccgtaag agataggagt tgaatcattc
     3541 ggatttctca ttcggtcgta aaattcttca gcttcagctt gtgtaacgcc ttcataaaaa
     3601 ttaactgccg aagtaagcaa taaatccact ccgtcagcct gattggtacg tttaggaaaa
     3661 actgtcggat cgaaaatcac aggagtcagt ttacgaatca aatcatcggg agtttcgccc
     3721 ttttcgagcg gcaaaacttc agcatcaata cttttcactg cctgagcaaa aaaagccgca
     3781 gaaaactttg gaacaaattt atcctgcgaa tagtgatgat gaataccgtt gcccatccaa
     3841 acctgcttca gataagtaat aaaatttctg aattcttcag actggcgatc tccgcgataa
     3901 ttttcgtaaa cagcttccaa tgtacgacga acac
//
LOCUS       DAOSIB010000125         3769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_21376_length_3769_cov_11.386645, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000125 DAOSIB010000000
VERSION     DAOSIB010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21376_length_3769_cov_11.386645"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..1409)
                     /gene="mtaB"
                     /locus_tag="PLD44_08395"
     CDS             complement(87..1409)
                     /gene="mtaB"
                     /locus_tag="PLD44_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOF99381.1"
                     /translation="MIDTNIYKTKKAAYYTLGCKLNFAETSQLGKQLSAAGYRKVHAG
                     EEADVCIVNTCSVTDTADHKCRQAIKRLKRQHPNAIMVVTGCYAQLNPEEIAAIDGVN
                     LVLGAGEKFDVVNFIEQWNAENSVEIHKTPIQKVKEFHPSCSGTDRTRYFLKVQDGCD
                     YYCTYCTIPFARGRSRNASIRETVELATKAVAEGAKEIVLSGVNIGDFGKSTGENFFD
                     LVRALDTLPEEVRFRISSIEPDLLTDELIEYIASSHHFMPHFHIPLQAGSNEALLLMK
                     RRYSTELFAHKVEVIKKWLPDAFIGVDVIVGTRGETEELFEKSKNFIASLDISQLHVF
                     TYSEREGTKMILMEQNVPLAERKRRSDELHLLSEQKTQLFYESQMGKEAEVLWEGRHN
                     GSKMVGFTANYIRVERPYEKKKVNTIEKIKLGGWNDQHSALIALGYHD"
     gene            1840..2181
                     /locus_tag="PLD44_08400"
     CDS             1840..2181
                     /locus_tag="PLD44_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOF99382.1"
                     /translation="MAYPKNKTYDIDIEELARFAKAMGHPARISILRYLASMDSCFFG
                     DICRELPIAKATVSQHLTELKNAGLIRGEIEPPKVRYCIDQENWEKAHEAFRQFFANM
                     QPKDICCNPED"
     gene            2192..2611
                     /locus_tag="PLD44_08405"
     CDS             2192..2611
                     /locus_tag="PLD44_08405"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOF99383.1"
                     /translation="MKILILCTGNSCRSQMAEAFLRSFDNRLTVCSAGTEPAEKVNEK
                     AIQVMREAGIDISRNRTKSVSQYLQETWDYVITVCADADRNCPVFSGKVKHRLHIGFD
                     DPAKVTGSEEFVLNEFRRIRDAIKEKFHQFYQENLQL"
     gene            2608..2964
                     /locus_tag="PLD44_08410"
     CDS             2608..2964
                     /locus_tag="PLD44_08410"
                     /inference="COORDINATES: protein motif:HMM:NF006749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HOF99384.1"
                     /translation="MNVIYTIVKVLLSSVVLVAVSEISKRSTFVGSLLASIPLVSLLA
                     IVWLYIDTKDTAQIASLSTGIFWLVIPSLVFFIVLPLLLKRSLNFWLSLIISSVLTII
                     AYFIMLYLLKKSGIKL"
     gene            2976..>3769
                     /gene="arsM"
                     /locus_tag="PLD44_08415"
     CDS             2976..>3769
                     /gene="arsM"
                     /locus_tag="PLD44_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOF99385.1"
                     /translation="MEKNIKSVVKDTYTVIAQQSKEQNETSCCGVGGCCSTVDYAVFS
                     ENYRQLKGYHEEADLGLGCGIPTQFASIKEGDSVLDLGSGAGNDCFVARAIVGETGKV
                     VGIDFTDAMLEKARKNNAKLGFQNVEFVKGDIEDMPLEDNQFDVVISNCVLNLVPDKR
                     KAFAEIKRVLKPGGHFCVSDVVVKGNLPEKLKNDAEMYAGCVSGAIEMQEYLDIIRET
                     GFQSVTVHKQKEVQLPQEILQKYLTEKEIRQFNQKEIGIFSITVAA"
BASE COUNT         1083 a          786 c          786 g         1114 t
ORIGIN      
        1 ctgaatatta tattcattcg tgtttgtgta tgcaatacat gtgggtttca taattgcgca
       61 aacggtattg atatgataag cttaaattaa tcatggtatc ctaaggctat taaggccgaa
      121 tgctgatcat tccagccgcc cagcttgatt ttttcgatgg tattgacttt ctttttttca
      181 taaggtcgtt ctacccgaat ataattggcc gtgaaaccta ccatctttga gccattatga
      241 cgaccttccc ataagacttc ggcttcctta cccatctgac tttcgtaaaa taactgggtt
      301 ttttgttcgg acaggagatg aagttcatca ctgcgccttt ttctttctgc caatggaaca
      361 ttttgctcca ttagaatcat ttttgttcct tctctttcgg aataggtaaa cacgtgcagt
      421 tgagagatat ctagcgaggc gataaaattt ttgctttttt caaaaagttc ttcagtttct
      481 ccacgtgttc ctacaattac gtcaactccg ataaaggcat caggcaacca ttttttaata
      541 acttcaactt tgtgagcaaa aagttcggtc gaatatcgtc ttttcatcaa tagcagggct
      601 tcattgctgc cggcctgcaa aggaatatga aaatgaggca tgaaatgatg actggaagca
      661 atatattcta tcaattcatc ggtcaacaaa tcgggttcaa tggacgaaat tctgaaacga
      721 acttcttcag gaagtgtatc caatgctctt accagatcaa agaaattttc tcctgtcgat
      781 tttccgaaat caccaatgtt gacaccggaa agcacaattt cctttgcacc ttcagcaacg
      841 gcttttgttg ccaattccac cgtttcccta atggatgcat ttctactcct gcctcgcgca
      901 aaaggaatag tgcaataggt acaataataa tcgcatccat cctgtacttt taaaaaatat
      961 cgtgtacgat cggttccgga gcaggaagga tgaaattctt tcactttttg aataggtgtt
     1021 ttatgaattt caacagaatt ttcggcattc cattgctcaa taaaattcac cacatcaaat
     1081 ttttcaccgg cacccagcac caaatttacg ccatcaattg cagcaatctc ttccggatta
     1141 agttgagcat aacaccctgt taccaccatg atggcattcg gatgctgacg tttgagtcgt
     1201 ttgatggctt gcctgcattt gtgatcggcc gtatcggtaa ccgaacaggt attgacaata
     1261 catacatctg cttcctcgcc cgcatgtact tttcggtatc ctgcagccga caattgtttg
     1321 cccaactgag atgtttcggc aaagttcagt ttgcaaccca atgtataata agccgctttt
     1381 ttggttttat atatatttgt gtcaatcatt tgttgcttac tcgaattctt ctgcaaaatt
     1441 acaaaaaaac agccaatttc tttttatcat atctttgttg aatcctctga aggaaaatat
     1501 tttctgatga gaatcaataa aaagttggtt gtaatacata aataaaaaat aatcgacaaa
     1561 aaggaaagaa gatgtggcaa tgattaaatt gttttggttt gatttcaatt cattactatg
     1621 taaaaaactt cgatttgttg cttttgcagt ctgggcgaag ttaaataagc gctacaggtg
     1681 ttgcttctcg ggcaaaaact ttgatgtgaa aatggacaat ctcaaacatt cagtttttaa
     1741 cttcccatta attgacttgc aaaaaaatat cattgatttt tttattttga atattattcg
     1801 taattttgcg aacaacaaac taaaattatc catagactaa tggcttaccc taaaaataaa
     1861 acctacgaca tcgacatcga agagttggcg cgttttgcca aagcaatggg acatcctgcc
     1921 cgcatcagca ttcttcggta cttggcttcg atggacagtt gtttcttcgg cgatatttgc
     1981 cgggaacttc ccattgccaa ggctaccgtt tcgcagcacc tgacagaact gaaaaacgcc
     2041 ggattaatca gaggcgaaat cgaaccgccc aaagtgagat actgcatcga tcaggaaaat
     2101 tgggaaaaag cccatgaagc ttttcggcaa ttctttgcaa acatgcaacc caaagacatt
     2161 tgctgcaatc ccgaagatta attttaccta tatgaaaata cttatccttt gcaccggtaa
     2221 cagttgccga agccaaatgg cagaagcatt tctgcgttcg tttgataatc gtttgacggt
     2281 ttgctctgcc ggtaccgaac cggcggagaa agtgaacgag aaggccattc aggtaatgcg
     2341 tgaagccggt atcgatatta gccggaacag aacgaaatcg gtcagtcagt atctgcagga
     2401 aacgtgggat tacgtgatta ccgtttgcgc cgatgccgac cgcaattgtc cggtattttc
     2461 gggaaaagta aaacaccggc tgcatattgg ttttgatgat ccggccaaag ttacaggttc
     2521 tgaagaattt gtcttaaacg agttcagaag aataagggac gcaataaaag aaaaattcca
     2581 ccaattctat caagaaaatt tacagctatg aatgtcattt ataccatcgt taaagtactg
     2641 ctttcgtcgg ttgtattggt agccgtttcc gaaatatcca aacggagcac ttttgtgggc
     2701 agcctgttgg cttccatacc gctggtttcg ctgctggcca tcgtttggct gtacatcgac
     2761 accaaagaca cggcacaaat tgcctcgttg tccaccggca ttttctggct cgtgattcct
     2821 tcgcttgtct tttttattgt gctgcccctg ttgctcaaaa ggagtctcaa cttttggctg
     2881 tcgctgatta tctcttcggt gctgacgatc atcgcttatt tcatcatgct ctatttactc
     2941 aaaaaatcag gaataaaact ctaacattca attctatgga aaaaaatatc aaatccgttg
     3001 ttaaggacac ttacacggtg attgctcagc aatcgaaaga acagaacgaa acttcctgct
     3061 gcggcgtggg cggatgttgt tctacggtcg attacgccgt attcagtgaa aattaccgcc
     3121 aactgaaagg ttatcacgaa gaagccgatt tgggattggg atgcggcata cctacccagt
     3181 ttgcgtccat caaagagggc gacagcgtgc tcgatttggg aagcggtgca ggcaacgatt
     3241 gttttgttgc ccgagccatc gttggagaaa ccggtaaggt ggtcggcatt gattttaccg
     3301 atgccatgct ggagaaagcg cgtaaaaata atgccaaact cggctttcaa aatgtagaat
     3361 ttgtcaaagg cgacatcgaa gatatgccat tggaagataa tcagtttgac gtggtgattt
     3421 ccaattgcgt attgaatctg gtgcccgaca agcggaaagc gtttgccgaa attaaacggg
     3481 tattgaaacc tggtggtcat ttttgcgtgt cggatgtggt tgtcaaaggc aatctgccgg
     3541 aaaagttgaa aaacgatgcc gaaatgtatg ccggttgcgt atcgggagcc atcgagatgc
     3601 aggagtatct ggatatcatt cgggaaacgg gatttcaatc ggttaccgtt cacaaacaaa
     3661 aggaagtgca actgccgcag gaaattctgc agaaatacct caccgaaaaa gaaatccgac
     3721 agtttaacca aaaggaaatc ggaattttca gcatcaccgt tgcggcaga
//
LOCUS       DAOSIB010000126         3697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_21939_length_3697_cov_9.188633, whole genome shotgun sequence.
ACCESSION   DAOSIB010000126 DAOSIB010000000
VERSION     DAOSIB010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21939_length_3697_cov_9.188633"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLD44_08420"
     CDS             complement(<1..485)
                     /locus_tag="PLD44_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HOF99386.1"
                     /translation="MNIKVTLLMMTLLSTAAAQTTENPFFQNYNTPHQTIPFHLIKNE
                     HYLPAFQEGIRQQEQEVEAIINNPQSPTFKNTIEALEYSGMLLEKVGAPFFNLLSAET
                     NDELQEIAQQVSPLLTEHSNSIFLNDKLFKKVKIVYDKREKLKLNTEQKRLLEKTYES
                     FV"
     gene            complement(535..1890)
                     /locus_tag="PLD44_08425"
     CDS             complement(535..1890)
                     /locus_tag="PLD44_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOF99387.1"
                     /translation="MKFFSSLLINVVSLTILLSFSQLMTAQEKNQLITELNVFDNDST
                     FKYSFIYNHDGKKSVENKYKLEKSHWIRSSQKEWNYEGENCISQTERKWDGSQWKPTY
                     TIEYRYENNLMTSESHYEYVNQFPRQISKIECFYENGRLISKKTYQLQDGTLKLQLEL
                     DYKYTTDQKIDSLFITSFSTATPLQYRSKFNYNLLGNVETQLLEKKNENGIWTKDEAT
                     RWYYNADSGQLTEQRKKRWNADFNFWENVQKTVYEYDNHYLKSEYSQYWNLMFWENAF
                     RYEYNYDTSGNLLNKILYLPIYNVWRKVITINYNNYEKGVSNVMESKYNFWGGSTDEL
                     VASDIPFLFNDEIDIRKAQRIEVGYSTFVDTITNSSNPDENHKNLVYPNPSEGIFYLD
                     TRHYKIQSFEVSNLSGKLLVVQNQPQETGIVDLTDLPRGIYIIKIQTIDNQIVQKIIK
                     K"
     gene            complement(2114..2185)
                     /locus_tag="PLD44_08430"
     tRNA            complement(2114..2185)
                     /locus_tag="PLD44_08430"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2151..2153),aa:Met,seq:cat)
     gene            2448..>3697
                     /locus_tag="PLD44_08435"
     CDS             2448..>3697
                     /locus_tag="PLD44_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOF99388.1"
                     /translation="MQNKGLIKIILGVLVIVSLYYLSFSFVSNRYSSKAKAYAGGDKE
                     KEYRYLDSLANEKVWLGYTLKECRERELGLGLDLKGGMNVILEVSVPDILRALSNYNT
                     TPNFTKALQIASERQKSSSQATYLDLFVQAYKELDPNAKLASIFSTYELKDKISLTST
                     NEEVVKVLRQEIDDAISNSFNVLRTRIDRFGVVQPNIQKIGSSGRILIELPGIKEPER
                     VRKLLQGSANLEFWETYDLSEIFNNLVEANRILASMSNIDSLNVAQKKDSLTAQTQTQ
                     PSTPAQKSAQKVSSVDSLKAALEKKNESAGDSLKLMEKQKKENPLFSLIQLNVMDGKI
                     GRGPLIGLVHSKDTSLVNSYFKMKQVREVLPRDLGLRWSVKSRDENGEWFELIALKVN
                     TRDGRAPLGGDVITDARADFDQTSA"
BASE COUNT         1118 a          646 c          693 g         1240 t
ORIGIN      
        1 acaaaacttt cgtacgtttt ctccaataac cttttttgtt cggtgtttaa tttaagtttt
       61 tctcttttat cgtaaactat tttgaccttt ttgaataact tgtcgtttaa gaaaatggag
      121 ttggaatgtt cggtcagtaa aggagaaacc tgttgtgcaa tttcctgaag ttcgtcattg
      181 gtttctgcac tcaaaaggtt aaaaaaaggt gcgccgactt tttccagtaa cataccggaa
      241 tattcgaggg cttctatggt gtttttgaat gttggcgact gaggattatt gataatggcc
      301 tccacttctt gctcttgttg acggattcct tcctgaaaag cgggcaaata atgttcgttc
      361 tttatcagat gaaaaggaat ggtttggtgt ggagtgttgt aattctgaaa aaaaggattt
      421 tctgtagttt gtgctgctgc ggtagaaaga agtgtcatca ttagtaatgt aacttttatg
      481 ttcatgctca attaacaatt aaactgttaa atatatattt ttgtaaatgg taatctattt
      541 ttttataatt ttttgaacaa tctgattgtc aatagtttgt atttttataa tatatattcc
      601 cctgggtaaa tcggtcaaat cgacaatacc tgtctcttgt ggctgatttt gaaccaccaa
      661 cagcttccca cttaaattgc tgacttcaaa ggattgaatt ttataatgcc gggtgtcaag
      721 ataaaaaatg ccttccgatg gattgggata aacgagattt ttatggtttt cgtcaggatt
      781 tgaactattc gtaatggtat cgacaaaggt tgaatatcct acttctatcc gttgtgcttt
      841 tctgatgtca atttcgtcgt tgaacaagaa tggaatatca gaagcaacca gttcatctgt
      901 agatcccccc caaaaattat atttcgattc cataacgttg gaaacgcctt tttcgtaatt
      961 gttataattg atggtaataa cttttcgcca cacattgtaa atgggtaaat acagtatttt
     1021 gttaagtaga ttgccggaag tatcataatt gtattcgtag cgaaaggcat tttcccaaaa
     1081 cattaaattc caatactgac tgtactccga tttcaaataa tgattatcgt attcgtaaac
     1141 ggttttttgt acgttttccc aaaagttgaa atctgcgttc catcgttttt ttcgctgctc
     1201 tgtcaattgt cccgaatctg cattgtaata ccatctggtt gcctcgtcct ttgtccaaat
     1261 tccgttttca ttttttttct ccaacaactg ggtttctaca tttccaagta agttataatt
     1321 gaactttgaa cggtactgca gtggggttgc agtggaaaat gaagtaatga acaaagaatc
     1381 gattttctgg tcagtagtgt atttgtaatc caactccaat tgcagtttca acgtcccatc
     1441 ttggagttga taagtttttt ttgaaatcag ccgaccgttt tcataaaagc attctatctt
     1501 gctgatttgc cggggaaact gattgacata ctcgtaatga ctttcggaag tcatcaaatt
     1561 gttttcatac ctgtattcga tagtataagt cggtttccac tgacttccgt cccattttct
     1621 ctcggtttga gagatgcagt tttctccttc gtagttccat tctttttgag aagagcgtat
     1681 ccaatggctt ttttcaagtt tatacttatt ttcgaccgat ttttttccat catgattata
     1741 aatgaaactg tatttaaaag tggaatcgtt atcgaacaca ttgagttcgg taattagctg
     1801 attcttttcc tgagctgtca taagctgtga gaaagagagt aaaattgtca gcgatacaac
     1861 atttataagt aaggaagaaa aaaatttcat gaatacacgc aaattggaga aaaatttttc
     1921 aaaaatacgc ataaatattt aatggaacaa attcatttct ttacctaaat taaagtgctt
     1981 atctactatt tctcaataat aaaaaaatca aatctctttt taaattgatc catgatttcg
     2041 atgctgagca acaaaaaaaa ggaatgcatc ttatagaaac attccttaaa tttttgaatc
     2101 gtaataatct ttgttgcgga ggcccgattc gaacgaacga cctttgggtt atgagcccaa
     2161 cgagctacca ctgctccact ccgcgatttt gtggtgcaaa agtacatatt tttttaaaaa
     2221 cttccaaatt ttttctgaac tcttttgtct ttaatttgaa attttttgaa gaatttcttt
     2281 taagcatttc tttatttgaa gtttatattt ttttcagccg gcataaaaat gatttttttc
     2341 acacaaactt cactgggcaa ttccaaattt tatgtattta ttaaaaaaaa tactatattt
     2401 gcacctcatt aaaaaaatgt caacattaat tttataaatc ttctcaaatg caaaacaaag
     2461 gacttatcaa gattatactt ggtgtgctgg ttattgtgtc tttatattac ctgtctttct
     2521 catttgtttc caacaggtat agctcaaaag ctaaagcata cgccggcggg gataaagaaa
     2581 aagaatatcg ttatctggat tcattggcaa acgaaaaagt ttggctggga tatacgctga
     2641 aagaatgcag agaacgtgag ctgggtttgg gactcgactt gaaaggcgga atgaatgtta
     2701 ttctcgaagt gtctgtgccc gatattcttc gtgcactttc caattacaac acaactccca
     2761 atttcacaaa ggccctgcaa attgcctccg agcgtcaaaa gtcaagcagc caggctactt
     2821 atctggatct ttttgttcag gcttataagg aactcgatcc aaatgctaaa cttgcctcca
     2881 ttttcagcac atacgaatta aaggataaaa tttctttgac atctacaaac gaagaagtcg
     2941 taaaagtact tcgtcaggaa atcgatgatg ccatcagcaa ttcattcaac gtattgcgta
     3001 cacgtatcga ccgttttggc gttgtgcagc caaatattca gaaaattggt agttcgggaa
     3061 gaattttaat tgagttaccg ggtataaaag aaccggaacg cgtacgaaaa cttttacagg
     3121 gttcggccaa tcttgaattt tgggaaactt acgatttaag cgaaatattt aataatttgg
     3181 ttgaagccaa ccgcattttg gcttcgatga gcaacattga cagtctgaat gtggctcaga
     3241 aaaaagattc attaactgct cagacacaaa cacaaccttc aactcctgca caaaaaagtg
     3301 ctcaaaaagt ttcttcggtt gacagcctga aagcagcttt ggaaaaaaag aacgagtctg
     3361 caggcgattc actgaaactt atggaaaaac aaaaaaagga aaatcctttg ttttcgctta
     3421 ttcagttgaa tgtaatggat ggaaagattg gacgcggccc acttatcgga ctggttcaca
     3481 gcaaagacac gtctttggtc aattcgtact ttaagatgaa acaagtgaga gaagttctgc
     3541 cacgcgattt gggactgaga tggagtgtaa aatcccgtga tgaaaatggt gaatggtttg
     3601 aactgatagc tctgaaagtg aatacgcgag atggacgagc accgcttggt ggtgatgtaa
     3661 ttacagatgc acgcgccgat tttgaccaaa cttctgc
//
LOCUS       DAOSIB010000127         3377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_24547_length_3377_cov_12.767911, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000127 DAOSIB010000000
VERSION     DAOSIB010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24547_length_3377_cov_12.767911"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..1710)
                     /gene="groL"
                     /locus_tag="PLD44_08440"
     CDS             complement(82..1710)
                     /gene="groL"
                     /locus_tag="PLD44_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941147.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOF99389.1"
                     /translation="MAKEILYNHDAREQLKKGVDELANAVKVTLGPKGRNVIIEKKFG
                     APHITKDGVTVAKEIELEDPFKNLGAQLLKEVASKTSDDAGDGTTTATVLAQAIVSVG
                     LKNVTAGANPMDLKRGIDKAVSQVVKHIASQSKKVDDDYNKIEQVATVSANNDSEIGK
                     LIADAMRKVSKDGVITIEEAKGTETTIELVEGMQFDRGYISAYFVTNTEKMLVEMEKP
                     YILLYDKKISNFKELLPILEPVAQSGRSLLIIAEDVDSEALTTLVVNRLRGSLKVCAV
                     KAPGFGDRRKEMLEDIAILTNGVVISEEKGLQLEKATMDMLGTADKVTVDKDNTTIVN
                     GAGSKEAIAARVSQIKSQIANTTSDYDREKLQERLAKLAGGVAVLYVGAPSEVEMKEK
                     KDRVDDALHATRAAIEEGIIPGGGVAYIRAIEALENLKGENEDEETGIEIVKRAIEEP
                     LRQIAFNAGKEGAVIVNKVKEGKGDFGYNARNEKFENFFETGVLDPAKVARIALENAA
                     SVAGMFLTTECVITEKKEENPVPQVPGGGMGDMM"
     gene            complement(1784..2056)
                     /locus_tag="PLD44_08445"
     CDS             complement(1784..2056)
                     /locus_tag="PLD44_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOF99390.1"
                     /translation="MNIKPLADRVLVKPAPAEEKTSSGIIIPDSAKEKPLKGEVVAVG
                     KGTKDEEMIVAVGDTVLYGKYAGTELEWDGEKYLIMRQSDIMGIIG"
     gene            complement(2229..3137)
                     /locus_tag="PLD44_08450"
     CDS             complement(2229..3137)
                     /locus_tag="PLD44_08450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOF99391.1"
                     /translation="MLSILIPVFNYSVSRLVGELHRQALQLFIDFEIIVMEDGSNEYL
                     EENRQIDKLSCCRYVVLEKNIGRSAIRNRLADMAKYGHLLFLDCDAEVCSAYFVEKYV
                     SFCREESVVVGGTAYDENFNDPAYSLRLKYGRLREARPATLRNKLELSGKSNFSAFNF
                     LIAKSIFNRIRFDETISGYGHEDTLFGHRIHELGFNVFHIDNPLIHKGLDDNPTFLFK
                     TEEGIRNLYYLYKTGRYPFLAKESSLLNSFLTLKKLSLIEFFSFLYRIFRKSIYANLC
                     SSNPSLILFDFYKLLYFCNYSQKMTR"
BASE COUNT         1019 a          711 c          562 g         1085 t
ORIGIN      
        1 taagaaaaat ttcagacaca aaaaaatctc cgaaaactat acagtctccg gagattttta
       61 acttatcgag aatttttaaa attacatcat atcacccatt cccccacctg gcacttgagg
      121 aaccggattc tcttctttct tttcggtgat cacacattcg gttgtcagga acataccggc
      181 cacagaggct gcattttcca aagcaatacg ggcaactttg gcaggatcga gtactcctgt
      241 ttcaaagaaa ttttcaaatt tttcgttgcg agcattgtac ccaaaatcgc ctttgccttc
      301 tttaactttg tttacaatta cggcgccttc ttttccggca ttgaatgcaa tctgacgaag
      361 cggttcttcg atagcccgtt taacaatttc tatacctgtt tcttcgtctt cgttctcacc
      421 tttcaggttt tccagcgctt cgatggcgcg aatataagca acaccaccac ccgggataat
      481 accttcttcg atggctgcac gagtagcatg taatgcatca tcaacgcggt ctttcttttc
      541 cttcatttca acttccgatg gagctcccac atacaataca gccactccgc ctgccaattt
      601 ggccaaacgt tcctgcaatt tttcgcgatc gtaatcggaa gtagtgttgg caatctgcga
      661 ttttatctga ctcacgcgtg cagcaatagc ttcttttgaa ccggctccgt ttacaatagt
      721 agtattgtcc ttgtcaaccg taactttatc ggctgttccg agcatgtcca tagtagcttt
      781 ttcaagctgc aaacctttct cttcgctaat tacgacacca ttggtaagaa ttgcgatatc
      841 ttcgagcatt tctttccggc gatcaccaaa tcctggagct tttacggcac aaactttcaa
      901 agaaccacgc aaacggttta caaccaacgt tgtaagtgct tcactgtcga catcttcggc
      961 aataatcaat aacgaacgac ctgattgtgc cacaggctcg agaataggaa gcaattcttt
     1021 gaaattggat atttttttgt cgtaaagcaa aatatatggt ttttccattt caaccaacat
     1081 tttttcggta ttggtaacaa agtaagccga aatataaccc cgatcgaatt gcattccttc
     1141 caccagttct atggtagttt cggttccttt ggcttcttca atggtaataa caccatcttt
     1201 ggagacttta cgcatggcat cggcaatcag tttaccgatt tcggagtcgt tgttggccga
     1261 aacggtagca acttgttcaa ttttgttgta atcgtcgtct acttttttcg attgagaagc
     1321 aatatgtttt acaacttgag acacggcttt atcgattccg cgtttcaaat ccattggatt
     1381 ggccccggct gtaacgtttt tcagtccaac acttacaatg gcttgagcca acactgtagc
     1441 tgttgtagtt ccatcaccgg catcatcgct tgttttggaa gctacttcct ttaaaagctg
     1501 agcgcccagg tttttgaatg gatcttccag ctcaatttct tttgctaccg taacaccatc
     1561 tttggttata tgaggtgcac caaatttttt ttcgattatg acatttcttc ctttaggtcc
     1621 caatgttact tttactgcat tagccaattc atcaacacct ttcttgagtt gctcacgagc
     1681 atcgtggtta tataaaattt ctttagccat attctttatg ttttaattta ataaacgttt
     1741 ttttaaattt atatttaagt tattaattat caagctttct tgcttaacct attattccca
     1801 taatatccga ctggcgcata ataagatatt tttctccatc ccattccagt tcggtacctg
     1861 catatttgcc atataacaca gtatctccca cagcaacaat catttcctca tcttttgttc
     1921 ctttcccaac agctaccact tcacctttca gtggtttttc tttagctgaa tcaggaataa
     1981 taattccact tgaagttttt tcttctgccg gagcaggttt taccaacaca cggtctgcta
     2041 aaggtttaat attcatatca cactaatttt taaatggtta ataaataaat ttatattttt
     2101 tgtcgtattt ttcgatacaa ataaataaat tcgctactgc ctgaatcaaa atgtgtgcct
     2161 gataaaaaaa tagatgatat tgacattttt gacagaaaga tggtggaaga aaaaaattac
     2221 taatactgtc atcttgtcat tttctgagaa tagttacaaa aataaagaag cttgtaaaaa
     2281 tcgaataaaa tcagggaagg atttgaagaa caaagatttg cataaataga ttttcgaaaa
     2341 attcgatata gaaaagaaaa gaattcgatg agcgacaatt ttttcaatgt cagaaacgaa
     2401 ttcagcaaac tggactcttt ggccagaaaa ggatagcgac cggttttata caaataataa
     2461 agatttcgaa ttccttcttc agttttaaac aaaaaggttg gattgtcatc caatccttta
     2521 tgaatcaacg gattgtcaat atgaaaaaca ttaaaaccca attcatgaat ccgatgtccg
     2581 aaaagcgtgt cttcgtgccc gtagccggaa atggtttcat caaagcgaat tctgttgaaa
     2641 attgattttg caattaaaaa attaaatgcc gaaaaatttg atttcccgct caattcgagt
     2701 ttgttgcgta aagttgccgg gcgggcttcg cgcaatctgc catactttaa tcgcaaacta
     2761 taggcaggat cattaaaatt ttcgtcatag gctgtgccac ccaccaccac agactcctct
     2821 cgacaaaagg aaacgtattt ttcgacaaaa taggcggaac aaacttcggc atcacaatcg
     2881 agaaaaagca aatgcccata ttttgccatg tcagccaagc gatttctgat agctgaacgg
     2941 ccgatatttt tttctaaaac tacgtatctg caacaactca atttatcgat ttgcctgttc
     3001 tcttccaagt attcattaga accgtcttcc attacaatga tctcaaaatc aataaaaagt
     3061 tgcagagcct gccggtgtaa ttcaccaaca agacgggaaa ctgaataatt gaataccgga
     3121 atcaggattg aaagcattat aaaagaaatt gaaaacttat cagaaatttt taacgctcac
     3181 aaaattaatt aaaaatcaac tgtattcaaa aaatttgatt cgataacaat ttttttgact
     3241 tcgcctctga taaaatggaa tctgaatatt taacaaaaag caaaattgaa gttgaggaag
     3301 gtaaatttta attgttacga tacctttcta taatttacac aaaagccaat ttaacagaaa
     3361 aagaaactgt ttgggca
//
LOCUS       DAOSIB010000128         3311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_25169_length_3311_cov_10.235872, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000128 DAOSIB010000000
VERSION     DAOSIB010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25169_length_3311_cov_10.235872"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLD44_08455"
     CDS             <1..282
                     /locus_tag="PLD44_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99392.1"
                     /translation="DMAFIAKSMNQDIFHAVSNDGRSITKAIEFLLPYVGKSLSEFPY
                     QQIEDWDKVQTDFCWVLKRADNLTETDNFKNIYSGFLPDSGKDLNYLIY"
     gene            322..1356
                     /locus_tag="PLD44_08460"
     CDS             322..1356
                     /locus_tag="PLD44_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99393.1"
                     /translation="MKKLILLLSFCVAVFTANAQIFTEYFETAPIGGDLEGYNDWYVC
                     LKSSDANGVSPKIEEGALFYTDYAGSNIGNVAVLDSAVGATSATQRISTKIVKFGSDT
                     LKAVVGEKIYTAFLANISNHSYRSYRDFFTYEGSKTSSMTRGRVFAKISTDGTELLIA
                     VTKNSSTSTDYVESTSIPGLTLYPDVNHLFVLVYETIDGSNNDKITLYIDPDLTKTEA
                     EQTNKLVAVDSQSDYSASAGLGINLRQRGISAQIGGIRVGKSWDEVLVEGTTAVNTPN
                     NSRAAIWTSGKNILTNTRGTIKIFNLAGAELISAQTDGCLTTNLSKGIYIVRFNGIDG
                     HTAVSKISLN"
     gene            1514..3202
                     /locus_tag="PLD44_08465"
     CDS             1514..3202
                     /locus_tag="PLD44_08465"
                     /inference="COORDINATES: protein motif:HMM:NF025943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chondroitinase-B domain-containing protein"
                     /protein_id="HOF99394.1"
                     /translation="MSFTIVNARQTTVTSASEINNGSWSAGDTIVMKNGTWTNQSISF
                     RGTGNESQPIVLMAETPGNVILEGTSKLQFSGKYLVVSGLYFKNGTLSGSDVISFRTS
                     SSQLAENCRLTNTAIENYNPLDSNVDSKWVSLYGKNNKVDHCSFVNKTNSGTLMVVWL
                     ISGVTVNHEISDNYFGYRNANLDANGDELNGQEIIRIGDSSTSMTTAAVTVKNNFFEH
                     CNGEIETISNKSCGNTYYNNLFYECKGMLTLRHGNGCTVKGNYFFGNGISNTGGVRII
                     GENHNVYNNYFENLRGTNYRAALCIVRGKENSALNEYFQVKNATVTFNTFVNCSQAFR
                     INYNSSSSLTMPPIGTVIANNHVYNTSSSYYNVSIDLTNATGMDVTWSNNLMNIGRYT
                     NFTYDENQVITGKDAKMIPVSTPIPMYEPSIGSELVNYVTADYPEIINDIRGRERNST
                     AKLPGASEINGIVSSSMPIKTLVGASFYNDPNTGIKNTNTSFKPFTWLIQRHQIIFNS
                     FETGNLYLVDMSGKVLLNWVVPLNTQLITKTLEKGIYLMVFKTPNKQYTEKIIF"
BASE COUNT         1118 a          563 c          631 g          999 t
ORIGIN      
        1 gatatggctt tcatagcaaa gtccatgaat caagatattt ttcatgctgt ttctaacgat
       61 ggcagaagta tcacaaaggc aatagagttt ttgctccctt acgtaggaaa gtctctttca
      121 gaatttccgt atcagcaaat tgaagattgg gataaagttc aaacagactt ttgttgggta
      181 ttgaaaagag cggataatct gaccgaaacg gataatttta aaaacatata ttccggattt
      241 ttaccggata gcggaaaaga cttgaattat ctgatttact aaaataaatt tttaatgttt
      301 cacttttaaa ttttttacat tatgaaaaag ttaattttac tactttcatt ctgcgttgct
      361 gtttttacgg caaatgcgca aatttttact gagtactttg aaactgcacc tatcggaggc
      421 gacttggaag gttataacga ttggtatgtt tgtctaaaat caagcgacgc taacggcgta
      481 agtcctaaaa ttgaagaagg ggctttgttt tacactgatt acgcaggctc taacattgga
      541 aatgttgcgg ttcttgattc tgctgtagga gcaacaagcg ccacacaacg gatctctact
      601 aaaattgtaa aatttggtag cgatacttta aaagcggtag ttggagaaaa aatttacaca
      661 gcatttctgg ctaatatttc caaccacagc tatcgttctt atcgcgattt ctttacttac
      721 gaaggttcta aaacttccag catgacacgt ggtagagtgt ttgcaaaaat aagtactgat
      781 gggactgaac tgttaattgc tgtcacgaaa aactcatcga catctacaga ttatgttgag
      841 tctacttcta ttccggggtt aactttatat ccggatgtta atcatttatt tgtattggtt
      901 tatgaaacaa tcgatggcag caacaatgat aaaatcactc tgtatatcga tcctgacctg
      961 acaaaaactg aagctgaaca aaccaataaa ttggtagcag ttgatagtca aagtgattat
     1021 agtgcgagtg ccggtttggg aattaatctg cgtcagcgag gaattagtgc tcaaattggt
     1081 ggaataaggg ttggaaaatc ttgggatgag gttcttgtgg aaggcaccac tgcagtgaat
     1141 acacccaata atagtagagc tgccatttgg accagcggta aaaatattct gaccaacaca
     1201 agaggaacaa taaaaatatt caatcttgcc ggagcagaat taatttcagc ccaaacggat
     1261 ggctgcttga cgaccaacct gtcaaaaggt atatatatcg ttcggtttaa cggtattgat
     1321 ggacatacgg ctgtatcaaa aatttctttg aattgagttt aattcaatga tatttaattt
     1381 atttttcatt gcttaagcat tctatcaggg aaaacagaaa aaaaatcaat ctgttttccc
     1441 tacttaaaaa aattttatga ttttaacttc atcgaacgca agcggcagac tattcttact
     1501 aataactttc tttatgagtt ttaccatcgt aaatgctcgg caaacgacgg ttacttcagc
     1561 ttcggaaatc aacaatggca gttggtcggc gggagacacg attgttatga aaaacggcac
     1621 atggaccaat caatccatat ctttcagagg taccgggaat gaatcgcaac caattgtact
     1681 aatggcagaa actccgggta atgtgatatt ggaaggaact tcaaagctcc aattttccgg
     1741 taaatacttg gttgtcagtg gactttattt caaaaatgga acattgagtg gttcggatgt
     1801 catttcattc agaacttctt cttctcaact tgccgaaaat tgcagactaa cgaatacggc
     1861 catagaaaat tataatcctt tggacagtaa cgttgatagc aaatgggttt ctctttatgg
     1921 aaaaaataat aaggttgatc attgttcttt tgtaaataaa accaattccg gaactctaat
     1981 ggtagtttgg cttataagtg gagtaacagt gaatcatgaa atttcagata attattttgg
     2041 ttatcggaat gccaatctcg atgcaaatgg cgacgaactg aacggacaag aaatcattcg
     2101 tataggagat agctctacct caatgacaac agccgcagta acagtcaaga ataatttttt
     2161 tgagcattgt aatggcgaaa ttgaaacgat ttccaataaa tcgtgtggaa atacgtatta
     2221 taacaatctt ttttacgaat gcaaaggtat gcttacatta cgtcatggga atggatgtac
     2281 cgtgaaagga aattactttt ttggcaatgg aatttctaat accggagggg taagaattat
     2341 tggagaaaat cacaacgttt ataataatta ttttgaaaac ctgcgtggta caaattatcg
     2401 agcagcactt tgcattgttc gcggtaaaga aaattctgct ctaaatgaat attttcaggt
     2461 gaaaaatgca actgtaactt tcaatacgtt cgtcaactgc tcccaagctt tcagaatcaa
     2521 ttacaacagc agttcttcac tgactatgcc accaatcggg acagtcattg ccaacaacca
     2581 cgtttataat acctcttcat cgtattacaa cgtaagcatt gatttgacaa atgcaactgg
     2641 aatggatgta acttggtcaa acaatttgat gaatattgga cgatacacaa attttactta
     2701 tgacgaaaat caagtaataa caggtaaaga tgcaaaaatg attcctgtaa gtactcctat
     2761 tcccatgtat gagccatcaa taggttcgga attggtaaat tatgtaacgg ccgattaccc
     2821 agaaatcatc aacgatataa gaggcagaga aagaaacagc accgcaaaat tgccgggagc
     2881 aagcgaaata aatggtatag tttcttcatc catgccgatt aaaactttag taggtgcttc
     2941 tttttataat gacccaaaca ccggtataaa aaacaccaat acatcattca agccatttac
     3001 atggcttatc caacgtcatc agataatttt caatagtttt gagactggaa atctatattt
     3061 ggtcgatatg tcgggaaaag tactgttaaa ttgggtggtt ccacttaaca cacaacttat
     3121 taccaaaacc ttagaaaaag ggatttactt gatggtgttt aaaactccga acaaacaata
     3181 caccgaaaaa attatttttt gaagaagtaa aacacaagag gaattaaaat atttaaaaaa
     3241 atttggatac tgcaattaaa acagtaattt tgtcgacact aattggtcga ccaatctatt
     3301 tcgttatgaa a
//
LOCUS       DAOSIB010000129         3223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_26087_length_3223_cov_9.431503, whole genome shotgun sequence.
ACCESSION   DAOSIB010000129 DAOSIB010000000
VERSION     DAOSIB010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26087_length_3223_cov_9.431503"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..1742
                     /locus_tag="PLD44_08470"
     CDS             333..1742
                     /locus_tag="PLD44_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99395.1"
                     /translation="MLNYFPKYFTQKAMLLYVISLAAVTVVFFSHAMSWIWIFFGLVE
                     VIGFFYFSNELTRQWGKTSPKTFTKRLFTNSLIIRIVWVIFSYFFYQSMTGQPFEFAA
                     ADAQGYHNEAVWLADMIHKGNIQPYFAYINGRFSDMGYPFYLGCLYAVTGKSILFARL
                     LKAVYGAITCVLIYKLTTRNFEESTGRMAGIFAMLMPNLIYYCGVHLKEAEMVLLTVA
                     FIERTDALLRNRKFNFINIFVPTLLGASLFFFRTALGITALFAVLTTLVFAHNKMVRS
                     WKRFIYGLWIGITIIYFAGTSISSEVESLWQSRKENQSTSIQWRAEREGGNRFARYAS
                     GTVFAPLILIIPFPTMVDIPGQENQQLLHGGNYVKNILALFVIFALIWVIKNKVWRNY
                     LLLGSFTIGYLLVMAFSAFAQSERFHQPALPFILILAAVGVSKSTNKTKKYFLWWLGF
                     IFVAIVGWNWFKLAGRGLT"
     gene            1894..2826
                     /locus_tag="PLD44_08475"
     CDS             1894..2826
                     /locus_tag="PLD44_08475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOF99396.1"
                     /translation="MRILFVCSKNSGKIAPFIQEQAEAIQQMGTEVDFFTIEGKGWKG
                     YLKNRPFLTKKIKEFKPNVIHAHYGLSGLLANAQRKVPVVTTYHGSDINDPKVFRFSK
                     WAMRLSAWNIFVSEKNIRIAKVKKKYTLIPCGINTELFKPIEKQVARKKFGFSTNEKL
                     VLFSGSFDNPVKNSALALQAINLLPEVQLLELKNYSREEVALLINAVDVCLMTSLSEG
                     SPQFIKEAMACNCPIVSVDVGDVKEQIEDVENCFIVEAQCEKIEDKLNGIFADGAKRT
                     NGREKLLRKGLEQSSVSEKLIKIYRRYGGTATSK"
     gene            2801..>3223
                     /locus_tag="PLD44_08480"
     CDS             2801..>3223
                     /locus_tag="PLD44_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF99397.1"
                     /translation="MVELLQANEIETDQWEQLVETSPVASFFQTPECYQFYASLTFLK
                     PFLYGVAEDGKLKGIACGYVQAEGGKLKQFFSRRAIIPGGILLDKDIEPGSITALLEF
                     LKKELHKRAIYIECRNFNDYSEYRPYFEKVGFIYQAHLN"
BASE COUNT         1063 a          524 c          663 g          973 t
ORIGIN      
        1 cgacagataa aaaacgcaga taaacacaga aacttatcag cgtaaatcag cgaataaaaa
       61 tcagcgaaaa tcagcgagaa aaaaatcggc ggaaatcagc gagaaaaaaa aataatctct
      121 cgcagataac cgcaaataaa aaaaagcaga tgaatgcaga aaacttatca gcggaaatca
      181 gcgagaaaga tatatctgtc agttaaaaca agagtatgaa acaatggata gtgttgaatg
      241 ataagtgata aatgaaaatt actcataact cgtaacttgt aactcaaaac ttggaattta
      301 aaactttcca gattagttat aaccaaaaaa gaatgctcaa ttattttccg aaatatttta
      361 cccaaaaggc catgctgctg tatgtcatat cattggcagc agtaacagtg gttttctttt
      421 cccatgccat gtcgtggata tggatatttt tcggattggt ggaagtaatc ggcttttttt
      481 atttttccaa cgaactaaca cgtcagtggg gtaaaacctc accgaaaact tttaccaaac
      541 gacttttcac caattctttg attatcagga tagtttgggt aatattcagt tattttttct
      601 atcaatccat gaccggtcaa ccatttgaat ttgcagctgc cgatgcacaa ggatatcaca
      661 acgaagcggt ctggttggct gacatgattc ataaaggcaa tattcaacct tattttgcct
      721 atattaatgg ccgtttttcc gacatgggat atcccttcta tctgggttgc ctatatgcag
      781 ttacgggtaa atcgattctc tttgcaagat tattaaaagc ggtttatgga gcgataactt
      841 gtgttctaat ttataaactt actaccagaa atttcgaaga atcaaccggc agaatggcag
      901 gtatttttgc aatgctaatg cccaatctga tttattattg cggtgtacat ttaaaagaag
      961 ccgaaatggt tttgctgacc gttgctttta ttgaaaggac agacgctctg cttcggaacc
     1021 gcaaatttaa ttttatcaat atatttgttc ctacactctt aggtgcttct cttttctttt
     1081 ttcgtactgc tttagggatt acagctttat ttgcggtatt aaccacgctg gtttttgccc
     1141 acaataaaat ggttcgcagt tggaaaagat ttatctatgg actttggatt ggaataacca
     1201 ttatttattt tgccggcacc agcatatctt ctgaagtgga aagtctatgg caaagcagaa
     1261 aagaaaatca atctacatct attcagtggc gagctgagcg ggaaggtggc aatcgttttg
     1321 cccgatatgc ttcaggaaca gtatttgccc cattgatact aataattcct ttcccaacaa
     1381 tggtggatat tccgggtcag gaaaatcaac agctattgca tggtggaaat tatgtaaaaa
     1441 atattttagc tctttttgtt atctttgctt taatatgggt aataaaaaat aaagtttggc
     1501 gcaactattt gttgttaggt tcgtttacca tcggttattt gttggtcatg gcttttagcg
     1561 cttttgccca gtcggagcga tttcatcagc cagctttgcc ttttattctg atattggcag
     1621 ccgttggagt gagtaagtcg acgaacaaaa caaaaaaata tttcctttgg tggttgggat
     1681 ttatttttgt agcaattgtt ggctggaatt ggtttaaact ggcagggaga ggattgacgt
     1741 gaaaaaatga ggagtattaa gtttagagta tgaagtgtag agtattgagt gtagaattgg
     1801 gagtagaaaa ttccgaattc tcataactca taacttaaaa ctcatgactc ggaacccagg
     1861 acttaaaaac gaaattatga atgacgaatt aacatgagaa tattatttgt ttgcagcaaa
     1921 aattcaggaa aaatcgcgcc atttattcag gaacaagccg aagccattca acaaatgggt
     1981 acggaagtgg attttttcac catcgaagga aagggctgga aaggctattt aaaaaaccga
     2041 cctttcttga cgaaaaaaat caaggaattt aaaccgaatg tgattcatgc tcactacggt
     2101 ttgtcagggt tactggctaa tgcacaacga aaagttccag ttgttactac ttatcacgga
     2161 agcgacatca acgatccaaa agtatttcgt ttttcaaaat gggcaatgcg attatcggca
     2221 tggaatatct ttgtgtcaga aaaaaatata cggatagcaa aagtaaaaaa aaaatatacc
     2281 ttgatacctt gtggtataaa cacagaactt tttaaaccga ttgagaaaca agtagcgcgt
     2341 aaaaaatttg gattttcaac taatgaaaag ttagtattgt tttcgggaag tttcgacaat
     2401 ccggtaaaaa attcggcttt ggctttacaa gctatcaacc ttttacctga agttcagctg
     2461 ctggaattga aaaattacag ccgtgaagaa gtggctttgt taataaatgc tgtagatgtt
     2521 tgtctaatga ccagcctcag tgaaggttca ccacaattta tcaaggaagc aatggcttgc
     2581 aattgcccga ttgtaagtgt agatgtaggt gatgtgaaag aacaaatcga agatgttgaa
     2641 aactgtttta ttgttgaagc tcaatgtgaa aaaattgaag acaaattaaa tggaatcttt
     2701 gcagatggcg ctaaaagaac aaacggacga gaaaaactgc taagaaaagg attggaacaa
     2761 tcttcggtat cagaaaagtt aataaaaatt tatcgtcgat atggtggaac tgctacaagc
     2821 aaatgaaata gagactgatc aatgggaaca actggttgaa acttctccgg tcgcttcctt
     2881 tttccagacg ccggagtgtt atcagtttta cgcatctctg acttttctga agcctttttt
     2941 gtatggagta gcagaagatg gaaagttaaa aggaattgct tgtggatatg tgcaggctga
     3001 agggggaaaa ttgaagcagt tttttagcag acgggctatt attcccggcg gaattttatt
     3061 ggataaggat attgaacctg gctcgataac agcattactc gaattcctaa aaaaagaact
     3121 tcataaaaga gccatttata ttgaatgcag aaatttcaac gattatagcg aatatcgccc
     3181 ttattttgaa aaagttggat ttatttatca ggctcatttg aat
//
LOCUS       DAOSIB010000130         3139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_26943_length_3139_cov_11.483463, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000130 DAOSIB010000000
VERSION     DAOSIB010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26943_length_3139_cov_11.483463"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PLD44_08485"
     CDS             <1..691
                     /locus_tag="PLD44_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOF99398.1"
                     /translation="INAIWKNKVAILSGDYYLTKVIEIAALTGRLDIITEIGKLGASL
                     ADGELVQLHVSQQQNPTEAIYFDIINKKTAQLFAFCAKAGAMASSAKPEAINRLALFG
                     EYLGIIFQLKDDSFDYILPGQSGEIPGKPVANDLKEGKVTLPLIYALEHSDEKKSRPY
                     REIMAKRHFTKYNVRRMYRFAIESGGLDYLSKKMEEYKQKAEKILAEFPPSDAKDSLN
                     LCLEYAANRCV"
     gene            complement(746..1075)
                     /locus_tag="PLD44_08490"
     CDS             complement(746..1075)
                     /locus_tag="PLD44_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOF99399.1"
                     /translation="MTLKDAQIQVDAWIKQYGVRYFNELTNMALLCEEVGEVARIIAR
                     EFGEQSKKADENRGKLSDELADVLWVLICLANQTGIDLEQAFAENIKKKTLRDKSRHQ
                     ENEKLHV"
     gene            complement(1078..1527)
                     /gene="dtd"
                     /locus_tag="PLD44_08495"
     CDS             complement(1078..1527)
                     /gene="dtd"
                     /locus_tag="PLD44_08495"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOF99400.1"
                     /translation="MKTVVQRVRRASVSVNKQLCGSIDKGLLVLIGIEDNDGPDDLIW
                     MAEKICHLRIFNDEKGVMNLSIMQDVGRLLVVSQFTLCANTQKGHRPSYIKAAKPEKA
                     KAMYEDFCSFCEKILNKKTEQGVFGAHMLVELINDGPVTILLDSKNR"
     gene            complement(1524..>3139)
                     /gene="uvrC"
                     /locus_tag="PLD44_08500"
     CDS             complement(1524..>3139)
                     /gene="uvrC"
                     /locus_tag="PLD44_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOF99401.1"
                     /translation="ALLLENNLIKEHQPRYNVLLKDDKTYPSICIKNECFPRVFQTRR
                     ILKDGSEYFGPYSSVPMVKAILDLIGKLHPLRSCSLDLSEKKISSGKYRVCLDYHIKK
                     CQGPCVGLQSEADYNKNIDEIRHILKGNLETVEEILKKEIKEHAEALRFEEAQNCKNK
                     MELLDSYRSKSYIVNSNIRELDVFSWDETENAAFINYLHVHNGCIVQTYTFEYRKKIE
                     EDKESLLASGIYEMRSRFKSESKEVVVPFIPSMELDNVKFHVPKRGDRKKLLDLSKNN
                     VRQYKIDLLKQEEKLNPEQRSTRILQTLKKDLQLSELPVHIECFDNSNIQGSSPVASC
                     VVFKKGKASKKDYRHFNIKTVPGIDDFASMKEIITRRYMRQAREEQGIPQLVIVDGGK
                     GQLSSAVEAHRHIIELCKKENIDEKILSKIQSIRLIGIAKRLEEIYFPNDPTPLYLNK
                     NSESLKLIQQIRDEAHRFGITFHRKLRSKVQIKSEMDEIPGIGKVTKNLLLEHFKSLK
                     KIREADSNELEKIVGKSKANNIINYFKSK"
BASE COUNT          854 a          640 c          573 g         1072 t
ORIGIN      
        1 tataaatgca atttggaaaa ataaggtggc cattttatcg ggagattatt atttaaccaa
       61 agtaattgaa attgccgcac taacgggtcg actggatatt ataacagaaa ttggaaagtt
      121 gggagccagt ctggccgatg gcgagttagt acaacttcat gttagtcagc agcaaaatcc
      181 tacagaagca atttactttg atataattaa taaaaaaacg gctcagcttt ttgccttttg
      241 cgcaaaagca ggagccatgg cttcttcggc aaaaccggaa gcgatcaacc ggttggcctt
      301 gtttggagag tatctgggca ttatttttca attaaaagac gattcattcg actatatttt
      361 acccggtcaa tccggagaaa tacccggaaa accggttgcg aacgatttaa aagagggaaa
      421 agtaacgctg cccctaatct atgctttgga acatagcgat gaaaagaaat cccgtccata
      481 tcgcgaaatt atggctaaac gtcattttac aaagtacaat gttcgtcgta tgtaccgctt
      541 tgctatcgag tcaggaggtt tggattactt atcaaaaaaa atggaagaat ataaacagaa
      601 agccgaaaaa atactcgctg aatttcctcc atcagatgct aaagactcct tgaatttatg
      661 cttggaatat gcggcgaatc gctgtgttta aactcataac cagtaacatt atctgctcaa
      721 aatcagcagg ttataaggct tccttctaca catgtaattt ttcgttttcc tgatgtctgc
      781 ttttgtctcg tagggttttt ttctttatgt tttcagcaaa ggcctgttct aaatcaattc
      841 cggtttgatt ggccagacaa atcaaaaccc acaagacatc ggccaattca tcgctaagtt
      901 ttcctctgtt ttcgtcggct tttttcgatt gttcgccaaa ttcacgggcg ataattctcg
      961 ccacttcgcc cacttcttca cacaataagg ccatattggt caattcgttg aagtaacgga
     1021 ctccgtattg tttaatccaa gcgtctacct gaatttgtgc atccttcaaa gtcattatta
     1081 tctgttttta gaatctagca aaatagttac agggccgtcg ttgataagtt caaccaacat
     1141 atgggcgcca aacactccct gttcggtttt tttgtttaat attttttcgc aaaaggaaca
     1201 gaagtcttca tacatagctt ttgctttctc gggcttagcc gctttgatat acgaaggccg
     1261 atgtcccttt tgtgtgtttg cgcacaaagt aaactggctt acaaccaata accgacctac
     1321 atcttgcatg atagaaagat tcataactcc cttttcatcg ttgaagatcc taagatgaca
     1381 aattttttct gccatccaga tcaggtcatc tggcccatcg ttatcttcaa ttccgatcaa
     1441 tacaagcaag cccttgtcga tactaccaca tagttgttta tttacagaaa cagaggctct
     1501 tcttactctt tgtacaactg ttttcatttc gatttaaagt aatttattat gttattggct
     1561 ttagactttc ccactatttt ttccaattca ttgctatcgg cttcccgtat ttttttcaaa
     1621 cttttgaaat gctccagtag cagatttttt gtgaccttac caatgcctgg aatttcatcc
     1681 atttccgatt ttatctgaac tttgcttctg agtttacggt gaaaagttat tccaaaacga
     1741 tgtgcttcat cccttatttg ttgtattaat ttcaaacttt ctgaattttt gttcaaatat
     1801 aagggagtag gatcgtttgg aaagtaaatt tcttcaagtc gttttgcaat gccaattagt
     1861 ctgattgatt gtattttact aagaattttt tcgtcgatgt tttctttttt acaaagctca
     1921 atgatgtgtc tatgagcctc cacagctgaa ctcaattgcc cctttccgcc gtccacaatg
     1981 accaattgtg gtatcccttg ttcttctctg gcctggcgca tatacctgcg agtaatgatt
     2041 tctttcattg atgcaaaatc atctattccg ggaacagttt taatattaaa atgcctgtaa
     2101 tccttttttg atgctttccc ctttttaaaa acaacacaag aagccacagg gctgctgccc
     2161 tgaatatttg aattatcaaa acattcgatg tgaacgggaa gttctgaaag ctgcaggtct
     2221 ttttttagcg tttgtaatat tcgggtgctt cgttgttccg gatttaattt ttcttcttgt
     2281 ttgagcagat cgatcttata ttgacgaacg ttgttttttg ataaatcaag cagctttttc
     2341 cgatcccctc gcttggggac atgaaattta acattatcca attccatcga aggaataaag
     2401 ggcaccacaa cttccttact ttcgcttttg aagcgactcc gcatctcata aataccactg
     2461 gcaagcagag attctttatc ttcttctatt tttttcctgt attcaaaggt gtaagtctgt
     2521 acaatacagc cattatgaac gtgaaggtaa tttataaagg cagcattctc tgtttcatcc
     2581 cagctaaaga catctaattc gcgtatgttg gaatttacta tataagattt actgcggtat
     2641 gaatccaaca actccatttt gtttttacag ttctgtgcct cttcaaatcg taaagcttct
     2701 gcatgttctt tgatttcctt ttttaatatc tcctccactg tttcaaggtt gcctttgaga
     2761 atatgccgaa tctcatcaat gtttttgttg taatcggctt cggactgtag cccaacacag
     2821 ggaccctggc attttttgat atggtaatcg aggcaaactc gatatttccc cgacgagatt
     2881 tttttctcag ataaatccag tgaacagctt cgtaatggat gtagtttgcc aattaaatcc
     2941 agaatggctt tgaccatggg tacagaactg tacggaccaa aatactctga cccgtctttt
     3001 aggattcttc gagtctggaa aactctgggg aaacattcgt ttttgatgca aatactgggg
     3061 taagttttgt cgtctttcag caaaacattg tacctgggtt ggtgttcttt tatcaggttg
     3121 ttttcgagca aaagagcgt
//
LOCUS       DAOSIB010000131         3075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_27656_length_3075_cov_12.529801, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000131 DAOSIB010000000
VERSION     DAOSIB010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27656_length_3075_cov_12.529801"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1293)
                     /locus_tag="PLD44_08505"
     CDS             complement(31..1293)
                     /locus_tag="PLD44_08505"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOF99402.1"
                     /translation="MQTMDSFNFAGKKAFVRVDFNVPLDENFNITDDTRIRAAIPTLK
                     KILNDGGCVIIASHMGRPKKNPDPKYSLKYILPHVSKLLGIEVKFAPDCIGPETAAMV
                     SALKPGEALMLENLRFYEEEEGKPRLPETATEEEKAAAKKELKERQKKFTATLASYAD
                     CYVNDAFGTAHRAHASTALIADYFDADHKMFGYLMEKEVAAVEKIMNNTEHPFTAIMG
                     GSKVSSKIDIIENLLNKVDNLIIGGGMTYTFVKALGGKIGSSICEEDKLDLALSLLEK
                     AKSKGVNMVLAVDAKIADAFSNDANTKFVPVNEIPDNWEGLDIGPETEKLFADVIKSS
                     KTILWNGPVGVFEFDNFTHGSLAVAQAIVEATKNGAFSLIGGGDSVACINKFGLADGV
                     SYVSTGGGALLEAIEGKVLPGIKAIRGY"
     gene            complement(1415..1834)
                     /locus_tag="PLD44_08510"
     CDS             complement(1415..1834)
                     /locus_tag="PLD44_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOF99403.1"
                     /translation="MATHITKTIWRKDNIFDTDVDGHIITIDLGKEAGGEDAGPRPKK
                     LLLVAAAGCSGLDVVSLLKKMRVELKGFDIQIEAQMSEEHPKQYTSMKVIYEFEGDNL
                     PMEKLEKAVTLSYETYCGVMAMYKKAIPISWEIRIKS"
     gene            complement(2570..>3075)
                     /locus_tag="PLD44_08515"
     CDS             complement(2570..>3075)
                     /locus_tag="PLD44_08515"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOF99404.1"
                     /translation="LIKQVIMKLSKFILAFLVLSALVFALPACKSKQKVVEIPGANAK
                     AGTSTSENQQKTTSPVIVTQPTTSTENVPEITRSESFSLAEGETNQSAMNYKYHVVVG
                     SFRNQSNAKGLQRTLSNEGYNPVIVINEQGMFRVIMNSFNTYNEAHVFISKIQSRFPD
                     AWVLIQK"
BASE COUNT          953 a          577 c          541 g         1004 t
ORIGIN      
        1 tatttaaaat atgaaaatca aagttgaaaa ttaatagcct ctaatggctt tgataccggg
       61 aagaactttt ccttcaatgg cttccagcaa agctccgcca cctgtagaaa cataagaaac
      121 gccatctgcc aaaccgaatt tgttgatgca ggcaacagaa tcgcctccac caatgagcga
      181 aaatgcacca tttttggtgg cttcaacaat agcttgagct acagccaaag atccgtgtgt
      241 gaaattatcg aattcaaaaa caccaacggg gccattccat aaaatggttt ttgaggattt
      301 tattacatcg gcaaaaagtt tctctgtttc ggggccaatg tccaaacctt cccaattgtc
      361 aggaatttcg tttacgggaa caaatttggt attcgcatcg ttactgaaag cgtcagcaat
      421 ttttgcatca acggccaata ccatatttac ccctttgctt tttgcttttt cgagcaatga
      481 aagtgccaaa tccaatttgt cttcttcgca aattgaactg cctatttttc cacccagtgc
      541 ttttacaaaa gtgtaagtca taccaccgcc aataatcaga ttgtcaactt tattgagcag
      601 attttcaata atatcaattt ttgaagaaac ttttgagcct cccatgatgg ctgtaaaagg
      661 atgttcggtg ttgttcatta ttttttcaac agctgccact tccttttcca ttaaatatcc
      721 gaacatttta tgatcggcat caaaataatc agcaatgagt gcggtagaag catgggcacg
      781 gtgagcggtt ccaaaggcat cattcacgta gcaatcggca taagaagcta gcgtagccgt
      841 aaattttttc tgacgttcct tcaattcttt ttttgcagca gctttttctt cttcggtggc
      901 cgtttcgggc aaacgcggtt ttccttcttc ttcttcataa aagcgcaaat tttccagcat
      961 tagagcttct cccggtttta aagctgaaac catagctgca gtttccggtc ctatgcaatc
     1021 gggtgcaaat ttaacctcta tacccaaaag ctttgataca tgtggaagaa tgtattttaa
     1081 ggaatatttg ggatccggat ttttcttggg ccttcccatg tgtgaagcaa taattacaca
     1141 accaccatca ttcaaaattt ttttaagtgt tgggatggct gcacgaatgc gcgtatcgtc
     1201 ggtaatattg aaattttcat ccagtggcac gttaaaatcc acacgaacaa atgccttttt
     1261 gccggcaaaa ttaaaagaat ccattgtttg catagtcttc aaatatttaa agtggtgatt
     1321 attttgtaaa ttcaatgaaa aagaaaactg ttgcaaattt agcaaaattt cgtggtttat
     1381 ttaacaaatg ggaaaagaaa agttttaagc ttgctcagga tttgattctt atttcccatg
     1441 aaataggaat agcttttttg tacatggcca tgacaccaca atatgtttcg taggaaaggg
     1501 taacagcttt ttccagtttt tccatcggta aattgtctcc ttcaaattcg tagattactt
     1561 tcatggatgt gtattgtttg ggatgctctt cactcatttg cgcttctatc tgaatatcaa
     1621 accctttcag ttccacacgc atttttttca aaagcgaaac aacatccagt ccactacaac
     1681 ctgccgctgc caccaacagc agttttttgg gacgtggccc agcatcttca cctccggctt
     1741 ctttgcctaa atctatggtt atgatgtgcc cgtcaacatc ggtatcgaaa atattatctt
     1801 ttctccaaat ggttttagtt atgtgtgttg ccataatgat tggtgttttt ttaagttttt
     1861 tctttatcca acaattttaa acgactttgg ttcgattctc tatttttctt tttatcaaat
     1921 taatattgaa ttattgagcc tgtaccgaat taattttgtt aatttttata caacagtata
     1981 gaataagtca gaaaaaagta gtgcttgcgc cgaagaaaaa ataagttctc actccattta
     2041 atcatcccat aaaaagttga ctattttggt ttgaaaaaac gggaaggaag taaattgatg
     2101 tatatgtaga aatagcttct accgcaaaca ataagttcct caaggaaagc atttttcaaa
     2161 gagaaaaaat atttgaccat tctgtagaaa aatgttttgc caatggtgct tatgctaggg
     2221 aagagaatca ggaatttgga atgtcaatca acaagcatct tattttttaa gtggcttgca
     2281 cgaagcaaaa gggaaatagg aactttcaaa aaattcacaa aataaaaata aaaaccaata
     2341 cctaacaaaa aaagtaaaac caagaaaaaa gagcagtatt aaaaatgata tattaaagac
     2401 aaaaatagcc agtttaaggt tgagaatgca caaacttctc ttttgtctgt tgagccaaaa
     2461 gccatgaaac attccaaaag aagaacttat cccagattaa gcattagcca aattaatagg
     2521 aaagtcaaac caagtcgtta tttcatggct ttgaaacaca catccctttt tatttttgaa
     2581 ttaaaaccca agcatcaggg aatcggcttt gtattttact gatgaaaacg tgtgcttcat
     2641 tgtaagtatt aaaagaattc ataattacac gaaacattcc ttgttcgttg attacaataa
     2701 ctggattata gccttcattg ctaagcgtac gttgtagtcc tttggcattt gattgatttc
     2761 tgaaacttcc tactaccaca tgatatttat aattcatagc gctttgattg gtttcaccct
     2821 cagccaaact gaaactttcg cttcttgtaa tttcggggac attttcagta cttgttgttg
     2881 gctgagttac tattacaggc gaggtggttt tttgttgatt ttctgaagtc gaagtacctg
     2941 cttttgcatt ggcaccggga atttcaacta ctttctgttt ggatttgcaa gcaggcaagg
     3001 caaatactaa tgctgaaaga acaagaaatg cgagaataaa tttgcttaat ttcatgatta
     3061 cttgttttat taaat
//
LOCUS       DAOSIB010000132         2962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_29051_length_2962_cov_13.229790, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000132 DAOSIB010000000
VERSION     DAOSIB010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29051_length_2962_cov_13.229790"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..886)
                     /locus_tag="PLD44_08520"
     CDS             complement(14..886)
                     /locus_tag="PLD44_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HOF99405.1"
                     /translation="MKVALIQQSNRQEISENLSKLQENIRKCAAAGAELVVLQELHNS
                     LYFCQTENPDYFDLAETIPGPSTDYFGKLAGELGVVLVLSLFEKRSAGLYHNTAVVLE
                     KDGTIAGIYRKMHIPDDPAYYEKFYFTPGDLGFVPIQTSLGKLGVMVCWDQWFPEAAR
                     LMALAGAELLIYPTAIGWETTDSEEEKQRQLEAWITIQRSHAIANGLPVVSCNRVGHE
                     DDPSGETGGIQFWGNSFVAGPQGEILVQASNQQEENLLVDVDLARSEQVRRIWPFFRD
                     RRIDSYQDLTKRWR"
     assembly_gap    1134..1233
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1456..2469)
                     /locus_tag="PLD44_08525"
     CDS             complement(1456..2469)
                     /locus_tag="PLD44_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOF99406.1"
                     /translation="MKKILPAEWEKQQFVQLTWPHSGTDFQPMLEDANRCFVNIAQAI
                     LQYEELLIVCAQAEEVKQMLAGEDFSRIRLVELPSNDVWARDHAGITVFENGRPVICD
                     FAFNGWGLKYPACHDNLITKRLFQAGVFKNYRYKNCLHFVLEGGSIDSDGAGTILTTA
                     GCLLSDNRNNATKKVIENRLKNYFGLERVLWLHHGHLAGDDTDGHVDTLARFCNEQTI
                     AYIKCEEEEDEHFEELKKMEMELQNFRTLQNEPYQLIPLPMADPVYENHERLPATYAN
                     FLIINGAVLMPTYQSPKDEIAKQRLQKAFPDRKIIGIDCRTLIKQHGSLHCVTMQFYP
                     AKG"
BASE COUNT          848 a          646 c          488 g          880 t
ORIGIN      
        1 cttgcaacaa actttatctc caccttttcg ttaaatcctg ataactatca atgcgcctgt
       61 cgcgaaagaa tggccagatt cgacgaactt gttcacttct tgccaaatca acatccacca
      121 gcaaattttc ttcctgctga ttggaagctt gaaccagaat ttctccctgt ggaccggcca
      181 caaaactgtt tccccaaaac tgaatgccac ctgtttcacc ggaaggatca tcttcgtgac
      241 caactcgatt gcacgaaaca acgggcaaac cgttagcaat ggcatgactt cgctgaatgg
      301 tgatccacgc ttcaagttga cgctgttttt cttcttcgga atcggtagtt tcccagccaa
      361 tagccgtagg ataaatcagc aattcagctc cggccaatgc catcaatcgg gctgcttcgg
      421 gaaaccattg atcccaacaa accatgactc ccaattttcc caaagaagtc tgaatgggta
      481 caaaacccaa atcgccgggc gtgaaataaa atttttcgta ataagccgga tcgtccggta
      541 tgtgcatttt tcgataaatt cccgctattg tgccatcctt ttcgagcacc actgccgtat
      601 tgtgatacaa gcctgcagat cgcttttcga ataacgataa aaccaaaacc acacccaatt
      661 ctccggcaag tttcccaaaa taatcggttg aaggtccggg aattgtttcg gcaaggtcaa
      721 aataatcggg attttcggtc tgacaaaaat aaagactgtt gtgcaattcc tgcaaaacga
      781 ccaattctgc acctgcggcg gcacactttc ggatattttc ctgcaatttg gacaaatttt
      841 cagaaatttc ttgcctgttc gattgctgaa tcaatgcaac tttcattttt ataatatttt
      901 tttgtgggtt atttgtgatt gaatttcaac ttaaagtcgt tggaaataaa taatttgctt
      961 gctaaaaagc aataacaatc ttttattcag acgttgcatg caacttctct acccggtatc
     1021 aaacccaatt caatgaaaaa accgtaattt ccaattcgtt tcatgcgttt tatagcattt
     1081 ttccaacaac atttttcacg catttttgta gagacgtagc atgctacgtc tcannnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcatttt tgtagagacg tagcatgcta
     1261 cgtctcaaac ctgatatatc gcaatccatt aaaaaaccgt aatttccaat tcgtttcatg
     1321 cgtttcattg catttttcca acaacatttt tcacgcattt tttagagacg tagcatgcta
     1381 cgtctctaaa aaactataaa ttattgagta tcaactatat gctaaaaatc caaactcttt
     1441 tctcgaaact tgtatctacc ctttcgccgg ataaaactgc atcgttacgc aatgcagcga
     1501 accgtgctgt ttgatcaacg ttctgcaatc gataccaata atttttctgt cgggaaatgc
     1561 tttttgtaac cgttgttttg ctatttcatc cttaggcgat tgataagtcg gcatcaaaac
     1621 agctccattg ataatcagaa aattggcata agttgccgga agtcgttcgt ggttttcata
     1681 aaccggatcg gccattggca atggaatcag ttgataaggc tcattttgta gggttctgaa
     1741 attttgcaat tccatttcca tttttttcaa ttcttcaaaa tgttcatctt cttcctcttc
     1801 acattttatg taagcaatgg tttgttcgtt gcaaaaacgt gccagcgtgt ccacatgtcc
     1861 gtccgtatcg tcgccggcca aatgtccatg atgcaaccaa agcacccgtt ccaggccgaa
     1921 ataatttttc aggcgatttt cgatcacttt tttcgtggca ttgtttcgat tgtcggacag
     1981 aagacaaccg gctgttgtca aaattgttcc tgctccatct gaatcaatac tgccaccttc
     2041 cagcacaaaa tgaagacaat ttttatagcg gtaatttttg aaaacgccgg cctgaaaaag
     2101 tcttttcgtt atcaaattat catggcaagc cggatatttg agtccccaac cattgaaagc
     2161 aaaatcgcaa atcaccggac gaccattttc aaaaaccgtg ataccggcat ggtcgcgtgc
     2221 ccatacgtca ttggaaggca gttcgaccaa ccgtattctg gaaaaatcct cgccggcgag
     2281 catttgtttc acttcttcag cttgagcaca aacaataagt aattcttcgt attgaagaat
     2341 cgcctgcgca atattgacaa aacagcgatt cgcgtcttcc agcataggct gaaaatctgt
     2401 accactgtgc ggccatgtta attgtacaaa ttgttgtttt tcccactcgg caggtaaaat
     2461 ctttttcatc agaatataat ttatttttta aaggtctgat ggaacccaca tgtctgaata
     2521 ttatattcat tcgtgtttga gtatgttata catgtgggtt tcataaaatt ttcatcaaaa
     2581 cattttgcac aaatatacag gataattctg aattgaacca gcagagaaag ataaattgaa
     2641 cttagaacaa aagtaaaatc ttcacctgca gcaaatcaat aaatttttcc ttttggatta
     2701 aaaaaatacc ataaaaaatt ttttaaaccg gttttcagca tttccatact tttataaata
     2761 attcaatatc atcattaatc gtataatttt tggtgcttaa atgcaaaaaa aacgaggcac
     2821 tgtcccgacg acaacgcccc gcttttaaaa aactgtcaaa caaaatgcaa cagctgcgaa
     2881 tttacaaaac aaccttaccc atccgattgc ccacttttac tatcatgata cctcgttcag
     2941 caatgcttac ggaattgata cc
//
LOCUS       DAOSIB010000133         2942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_29315_length_2942_cov_14.281261, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000133 DAOSIB010000000
VERSION     DAOSIB010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29315_length_2942_cov_14.281261"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..716)
                     /locus_tag="PLD44_08530"
     CDS             complement(228..716)
                     /locus_tag="PLD44_08530"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HOF99407.1"
                     /translation="MKKNGVLILTILLLASFRAGAQTIDKFFDRYANDDRFTYVTVGK
                     GMMNLASTFGANKNMDKNQKEVLDRLTGIKILTLENSRDEKLMNSVLDELDKVIRAGK
                     YETAVRVKDKDETVNIFFRTTSKDQSDMIIVSKEKNEMSIIWMNGKMTKEEMSDIFSQ
                     NS"
     gene            complement(754..1239)
                     /locus_tag="PLD44_08535"
     CDS             complement(754..1239)
                     /locus_tag="PLD44_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99408.1"
                     /translation="MKEIDDLLNKYFEGKTTLEEENYLKSYFRNEEIQPEHEVYKTLF
                     HVFDEKAKEKIPQPVDEKNAQPNVKRFWKLTAVITGIAAAFLMIIWVMPIEKNQDFAI
                     VNGEKIENTQFAQNYALEKLNHVSYKLETNLKPLEAIATVKSRMEPVKKIPEIKKEWK
                     K"
     gene            complement(1289..1798)
                     /locus_tag="PLD44_08540"
     CDS             complement(1289..1798)
                     /locus_tag="PLD44_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOF99409.1"
                     /translation="MDAAQFNSVILPLADQLFRVAKSIVCNDEAAKDAVQELNAKLWE
                     NRSQLDKVQNLTAFAMKSMRNLCLDWLRTRKSDYDVPLDENLIASAPNPHQLMEQKDL
                     TNYVKQLIDKLPELQRTIIHLRDVEGLEISEIAAITSISENAISVNLSRARQKLRTEL
                     MEKSKKEKI"
     gene            complement(1947..2723)
                     /locus_tag="PLD44_08545"
     CDS             complement(1947..2723)
                     /locus_tag="PLD44_08545"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOF99410.1"
                     /translation="MNGIVNLSLVPLRAEDNECSEMISQLLFGETVEIMEWKERWLHI
                     RNLSDNCCGWADRKMITLLSEDEYENLKNNENFRILKAVSFCRNLTLGTTFLLPGGSI
                     LRKFQNGNFTDGTYQYAIHPSDAGMPKPLTGGNIVSLARQYHFTPYLWGGKSALGIDC
                     SGLVQVVFSLCGVLLPRNASQQAEIGEKINSLTQAEAGDLAFFANSAHHIVHVGILMN
                     SHQIIHASGWVKIENIDEHGIISEQTGNYSHTLYSIKRMI"
BASE COUNT          845 a          572 c          503 g         1022 t
ORIGIN      
        1 taatgtaatg cttgcagcag ctgcatttaa cttcaaaagg atgatgaata aatggaaacc
       61 atctttttag ctcaaactta aaatacaaat tgtttctcaa aatcgcttta aataaaaaaa
      121 caagcctggt tttcgagcct atggaacata tcttcagagt ttgactttcc ttataaattg
      181 gctaatgagt aatctgggtt aataaacaaa aatataaaat aagtttttca ggaattttgc
      241 gaaaaaatat cggacatttc ttcttttgtc atttttccgt tcatccatat tatcgacatt
      301 tcgtttttct cttttgacac aataatcata tcggactgat ccttgctggt tgtcctgaag
      361 aagatgttca ccgtttcatc cttatctttc actctgacag ccgtttcgta tttcccggcc
      421 cgaattacct tgtccaattc atcaagaacc gagttcatta atttttcatc gcgtgaattt
      481 tccaaagtta aaattttgat tcccgtcaaa cggtctaaaa cttccttctg gttcttgtcc
      541 atatttttat ttgctccgaa tgttgaagca aggttcatca ttcctttacc gacggtaacg
      601 taagtgaatc gatcgtcatt ggcatatcga tcaaaaaatt tatcgatggt ttgtgcacct
      661 gctcggaaag atgcaagcag caaaattgtc aaaattaata ctccattctt tttcatattc
      721 gtgattgtta attgtttaaa tgaattgttt cttctatttt ttccattctt tttttatttc
      781 cggaattttt ttcaccggtt ccattctcga tttaacggtc gcaattgctt ccaaaggttt
      841 taaatttgtt tccaacttgt agctgacatg gttcaatttt tccaaagcat aattttgagc
      901 gaattgtgta ttttcaattt tttccccgtt cacaatggca aaatcctgat ttttctctat
      961 cggcataacc caaataatca tcagaaaagc tgcagcaatt cccgtaatga cggcagttaa
     1021 tttccaaaac cgtttcacat tgggttgtgc atttttttcg tcaaccggtt gaggtatttt
     1081 ttctttggct ttttcatcaa acacatgaaa tagagttttg tagacttcat gttcgggctg
     1141 tatttcctca ttcctgaaat aagatttcag gtaattttcc tcttcaagag tggtttttcc
     1201 ttcaaaatat ttatttaata aatcgtcaat ctctttcatt gcaagtcgtt ttttaataaa
     1261 ttgataaaac gattattcaa aatttccttc atattttttc ctttttcgat ttctccatca
     1321 attctgtcct taatttttgc cgtgctctcg agagattaac acttatggcg ttttccgaaa
     1381 ttgaagtaat tgctgctatt tccgaaattt ccaaaccttc cacatcgcga aggtgaataa
     1441 tggttctttg caattcgggt aatttgtcta tcaattgttt cacatagttt gtcagatctt
     1501 tttgttccat caactgatga gggttgggcg cagatgctat cagattttca tccagaggaa
     1561 catcataatc tgattttctt gttcgcaacc agtcgagaca taaattgcgc atggacttca
     1621 tggcaaaagc cgtaaggttt tgcactttat ccagttggct gcgattttcc cacaattttg
     1681 cgttcaactc ctgtacggca tctttggcag cttcatcatt gcagacaatg gattttgcca
     1741 cccgaaacag ttggtcagca agtggcaaaa taaccgaatt aaattgtgct gcatccatac
     1801 attactgaat tctcactaag ttaccaaatt tgattcatac acctataatg acgaaaaacc
     1861 ggatgatttc ttacatcaaa tactcgattt tttttaaagg taaggagaat tttttttgaa
     1921 gagagtctga cgattttgaa aatgatttaa atcattcttt tgatggagta aagggtatgg
     1981 gaatagtttc ctgtttgttc ggaaataatt ccatgttcat caatattttc gatttttacc
     2041 cagcctgatg catgaataat ttggtgagaa ttcattagaa tacccacatg aacaatgtga
     2101 tgagcagaat tggcaaagaa agccaaatct ccggcctcag cctgagtaag agaatttatt
     2161 ttttcaccga tttcggcctg ctgagaagca ttgcgcggta acaagacgcc gcaaagcgaa
     2221 aaaacaacct gaaccaaacc tgaacaatca attcccaatg cactttttcc gccccacaaa
     2281 tagggtgtaa aatgatattg tcgagctaaa gaaacgatat tgcctccggt taaaggtttt
     2341 ggcattccag catcggaagg atgaatggca tattgatatg tcccatctgt aaaattcccg
     2401 ttctgaaatt ttctcagaat gcttcctccg ggcaggagaa aagtagttcc caatgtcaga
     2461 ttgcgacaaa atgaaaccgc tttcagaatg cggaaatttt cgttattctt gaggttttcg
     2521 tattcatctt ccgaaagcaa agtaatcatt tttctgtcag cccagccgca acaattgtcc
     2581 gaaagattgc gaatgtgcag ccatcgttcc ttccattcca ttatttcaac tgtttctcca
     2641 aacagaagct gagaaatcat ttcgctgcat tcgttgtctt ctgcccttaa aggaacaagg
     2701 ctcaagttca caattccatt cataaataca ataatttata cgtttgatat tagtacatgg
     2761 caaaaattga gacttttatg ttatatatct gtaaaacaga acaattatgc taagttttgg
     2821 gtttgacttt gtccggtgag ccaaaaaaca agtgagaaaa gcgtttaaaa atcgtcctta
     2881 ttcgagctcc gacgtatttc gtcggaatga gtttgggcga tttcagcttt tcgagcgtaa
     2941 gt
//
LOCUS       DAOSIB010000134         2868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_30268_length_2868_cov_10.303235, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000134 DAOSIB010000000
VERSION     DAOSIB010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30268_length_2868_cov_10.303235"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLD44_08550"
     CDS             complement(<1..572)
                     /locus_tag="PLD44_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOF99411.1"
                     /translation="MLKPEYIFETSWEVCNMVGGIYTVLSTRAATLKEVYGDKLIFIG
                     PDIWIQKNNPLFVEDSRLFPGWKDAVQQQFQLNIRIGRWQIHGNPIAVLVDFMPLMLR
                     KDEIYGHVWLQTGVNSLPAYGDYHESSMFGYAAGMVIESFYRFFGLSLKNDVIAHFHE
                     WQTTFGIFYLKEHLPEAATIFTTHATTVGRS"
     gene            complement(808..1416)
                     /locus_tag="PLD44_08555"
     CDS             complement(808..1416)
                     /locus_tag="PLD44_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOF99412.1"
                     /translation="MKNFLFVSNIVLFIAVVVLFVLFFQIKPAKNAVQLSLKGDTLSS
                     QVLPIAYINVDSLLLKYRFAKDANESLIKKQEDSRLTINSRARELQNEMNEFQRKLEN
                     NAFLSRERAEQEHTRLLKKQQELQNLDGQLTQQLLQEQQKMSEELRDTINAFLKEYNK
                     DGRYEIIFSNTTGDNILFASKSYDITDEVVKLLNERFSNKKK"
     gene            complement(1500..1745)
                     /locus_tag="PLD44_08560"
     CDS             complement(1500..1745)
                     /locus_tag="PLD44_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99413.1"
                     /translation="MFYTLLITLLIVLLSVLLLGFRIFFIKSGKFPNIHIGGSKALQQ
                     RGIGCATTQDKEAQRNFHKGIDVSNLLTEISEQLKNE"
     gene            complement(1933..2508)
                     /locus_tag="PLD44_08565"
     CDS             complement(1933..2508)
                     /locus_tag="PLD44_08565"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008778337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOF99414.1"
                     /translation="MKFQLPELPYAQNALEPVISERTISFHYGKHHLTYVNNTNNLVV
                     GTEFENQDLETIVKKATGPLFNNAAQIWNHNFYFLSLTPDKGTKPSEKLLKAIEAEWK
                     SLDNFKIEFGKAATGLFGSGWAWLVKDASGKLSIVNESNAGNPMTKNLSPILTFDVWE
                     HAYYLDYQNRRADYVAALWDLVNWSIVSERF"
BASE COUNT          980 a          507 c          458 g          923 t
ORIGIN      
        1 gaacgtccca cagtagtggc atgagtagta aaaatggttg cagcttcagg caaatgttct
       61 tttaagtaaa aaataccgaa agttgtttgc cactcatgaa aatgggcaat cacatcattt
      121 ttcaaagaca aaccaaaaaa tcgataaaaa ctttctatca ccatacctgc agcatagcca
      181 aacatcgaag attcgtgata atcgccatag gcaggcaagg aattgactcc ggtttgcagc
      241 caaacatggc cataaatttc atcttttcgt agcatcagcg gcatgaaatc gaccaatact
      301 gcaatcggat ttccatgaat ttgccaacgc cctatacgaa tattcaactg aaactgttgc
      361 tgaacagcat ctttccaacc ggggaaaagc ctactgtctt caacaaaaag cgggttgttt
      421 ttttgtatcc atatatcagg tccaataaaa atcagtttgt ctccataaac ttctttcaat
      481 gttgcagcac gagtagataa aacggtatat attccaccaa ccatattgca tacttcccag
      541 cttgtttcaa atatgtattc cggcttcaac atacttaatt tggtataatc gaaattaaac
      601 tttctgttgt tttttgaaaa cggggaaaaa ttgaaaataa aactgataaa aattaattat
      661 cttatcaatg attttaaaat caaacaaaag tgataaaaaa gtatttttga aaaaactaaa
      721 tatttgtttt atgaatatac tataccttgc ttgtcaaaaa aaatcctact tacgtaggat
      781 atactttttg aacagccaaa ttagaaatta ttttttcttg tttgaaaatc gttcgttcaa
      841 cagtttcacc acttcatcgg taatatcgta acttttggaa gcaaatagga tattgtcgcc
      901 tgtggtatta ctgaaaataa tttcataacg gccatcctta ttatattctt tgagaaaggc
      961 attaatggta tcgcgcaatt cttcactcat tttttgttgt tcctgtaaaa gctgttgagt
     1021 aagttgtcca tcaagatttt gtaattcttg ttgttttttt aataaacgcg tatgttcctg
     1081 ctctgcccgc tcccggctga gaaatgcatt gttttcgagt tttctctgaa attcgttcat
     1141 ttcattttgc agttcacgag cacgagagtt tatggtcaac cgtgaatctt cctgtttttt
     1201 aatgagggat tcattggcat cctttgcaaa ccggtatttt aataacaagg aatctacatt
     1261 tatgtaagca ataggtaaaa cctgacttga cagggtatct ccttttaatg acaactgaac
     1321 cgcattctta gcgggtttga tctgaaaaaa caaaacaaaa agtacaacaa cagcaatgaa
     1381 aagaacgatg ttggaaacaa ataaaaaatt tttcatattt aagctttaaa atattatctt
     1441 taattcatac catgcaaaga caaaaataaa attcaaaaag acaatctgca tgaaacaacc
     1501 tattcgtttt tcaattgttc agaaatttca gttaacaaat tggaaacatc aattccttta
     1561 tgaaaattcc gttgggcttc tttgtcctgt gtggtagcac agccaatacc tctttgttgg
     1621 agggctttgc ttccaccaat atgaatattg ggaaattttc cacttttaat gaagaagata
     1681 cgaaatccca ataataaaac cgaaagtaaa acaatcagta aggttataag taaggtgtaa
     1741 aacataaact taaaaatata acggcttgca aagataagat atttaataga aaatgaaatt
     1801 atttctatga ttttttagca aaacaaataa tttatttgct ttattagtaa ttcttaaata
     1861 tttatgcaat aaattaaaaa ccggcctatg ttataagccg gtaaaaaaac aaaaaggtaa
     1921 aaaacaaaat aatcagaaac gttccgacac tatgctccaa tttaccagat cccataaagc
     1981 agctacataa tccgctctcc ggttttggta atcgagataa taagcatgtt cccaaacatc
     2041 gaaagtaaga atgggagaca gattttttgt catcggattc cccgcattac tttcgttgac
     2101 aatggaaagc tttcccgaag catcctttac gagccaagcc cagcctgaac caaacaatcc
     2161 ggtcgcagct ttgccaaatt caattttgaa attatccaac gatttccatt cggcttcgat
     2221 ggctttcagt agtttttcgc tgggtttagt acctttatca ggggtcaagg aaaggaaata
     2281 gaaattgtga ttccatattt gtgcggcatt gttgaaaagc ggacctgtcg cttttttaac
     2341 aatggtttcc aaatcctgat tttcaaattc tgtacctact accaaattgt tggtgttgtt
     2401 tacgtaagtt agatgatgct tgccgtagtg aaaactgatt gttctttcac tgataaccgg
     2461 ttccaaagca ttctgtgcat aaggtaattc aggcaattga aatttcataa caataaaatt
     2521 ttatggttaa tactaaattg attttataga aaatttattg ttataacaat caattttctg
     2581 ttttgtttta atatttaccc aaaaaaattc aacggatatt atctcttact tttcaaaatt
     2641 tctatagtcc tgttaaaaaa atccaaataa ttatctttca aataaataac tataccaatt
     2701 tttttaagta aagaattttt ttgaccaatg gaatttgaag tcgataaagg gaaacgattc
     2761 aatatgttta tatacaaaga aagccctgaa atacaggact tttcagcgga gagagaggga
     2821 ttcgaacccc cggaaccttt cagttcaacg gttttcaaga ccgccgca
//
LOCUS       DAOSIB010000135         2767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_31722_length_2767_cov_12.472345, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000135 DAOSIB010000000
VERSION     DAOSIB010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31722_length_2767_cov_12.472345"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..909
                     /locus_tag="PLD44_08570"
     CDS             37..909
                     /locus_tag="PLD44_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOF99415.1"
                     /translation="MMKYFAPSELLINDDGSIFHLHLKPEHLADKIILVGDPGRVEKV
                     AAHFDSKECSVSNREFNCITGTYQNKRITVVSTGIGTDNIDIVMNELDALANIDLKNR
                     TEKTDFRQLTIVRIGTSGGMQPEIPLGSFLISEKSIGIDGLLSFYARRDEVCDLDFEK
                     AFKKHTKWNPQLASPYVVDNDMELIERIGKDDMLRGVTISANGFYGPQGRVLRLDLHD
                     SHLNDKIESFRYKDYKITNFEMESSAIAGLSKLMGHKAMTVCCIIANRRVESANTNYQ
                     PHIEKLIQTVLDRI"
     gene            928..2325
                     /gene="mnmE"
                     /locus_tag="PLD44_08575"
     CDS             928..2325
                     /gene="mnmE"
                     /locus_tag="PLD44_08575"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOF99416.1"
                     /translation="MNTASTICAISTPPGTGGIAVIRVSGQNAFSCVNKFFVPRKKDT
                     DIFQQSPYTLNLGSFMNARKETIDDVLIGIFRAPHSYTGEDVVEISCHGSVYIQQQIL
                     QTLIDAGCSLAKPGEFTQRAFLNKKMDLSQAEAVADLIAADSAASHRMALNQMRGGIS
                     EELKNLRTRLLDFVSLTELELDFSEEDVEFADRSQLMALANEIEELILKLSNSFKVGN
                     ALKNGIPVAIVGETNVGKSTLLNVLLNEDKAIVSDIHGTTRDAIEDSVNIDGITFRFI
                     DTAGIRETKDEIETLGIARTYQKIEQASIILWLIDCTQLSERIETLTEKIAKKIQDKK
                     FILIFNKIDKITDEEREVLNQFFEHYSGERIYLSAKKRINIHQLQKLLVKAAQLPEVQ
                     PGDVVVSNVRHYEALQNALKAIQRVKEGIQNGISGDFLSQDLRECMFFLGEITGEISS
                     DEILGNIFSKFCIGK"
BASE COUNT          911 a          484 c          528 g          844 t
ORIGIN      
        1 caacaataaa acgaattaat tttttaaaac agaaatatta tgaaatactt tgctccttct
       61 gaacttctta tcaatgatga tggaagtatt tttcatctgc atctgaaacc cgaacatttg
      121 gccgataaaa ttattttggt tggagacccg ggacgtgtag aaaaagttgc tgcacatttt
      181 gactcgaaag aatgttcagt ttcaaacaga gaatttaact gtatcacagg tacttatcaa
      241 aataagcgta ttacggttgt ttctaccggc attggaacgg ataacattga cattgtcatg
      301 aatgagctgg atgctttagc caacattgat ttgaaaaata gaaccgaaaa aacagatttc
      361 agacaactca ccattgttcg gattggaact tccggcggaa tgcaacccga aattcctttg
      421 ggaagttttc tcatttcaga aaaatctatc ggaatagatg gtttattgag tttttatgcc
      481 cgcagagacg aagtttgtga tttggatttt gaaaaggctt ttaaaaaaca tacgaaatgg
      541 aatccgcaat tggcttctcc ctacgttgta gataatgata tggaattaat cgaacgaata
      601 gggaaagatg acatgttaag aggtgtaacc atttctgcca atggatttta tgggcctcag
      661 ggaagagtac tccgtctcga tttgcacgac agtcatctga atgataaaat tgaaagtttc
      721 cgatacaaag attataaaat taccaatttc gaaatggaaa gttctgcaat tgccggtctc
      781 tccaaactca tgggacataa ggccatgacg gtttgctgca ttattgccaa tcgcagggta
      841 gaatcagcca atacaaatta tcagccacat attgaaaaac tcattcagac tgttttagac
      901 agaatttaac gaaattttta tttcttaatg aacacagcat ctacaatttg tgcaatttct
      961 actccacccg gcacaggagg catcgctgtc atccgtgtgt cgggtcaaaa tgctttttct
     1021 tgtgtcaata aatttttcgt tccaagaaaa aaagatactg atatttttca acaatcgccc
     1081 tatacgctca atctgggcag ttttatgaat gctcgaaaag agacaattga cgatgtgttg
     1141 attggaattt ttcgagcacc tcactcctac accggtgaag atgttgtgga aatatcgtgt
     1201 catggttctg tgtacataca acagcaaatt ttacagacat taatcgacgc aggctgttca
     1261 ttagccaagc cgggagaatt tacgcaaaga gcttttttga ataaaaaaat ggatttgtcg
     1321 caagctgaag cggttgccga tttgattgct gccgattcgg cggcctcgca ccgaatggca
     1381 ttgaaccaaa tgagaggtgg catctcggag gaattgaaaa atttacgtac acggttgctc
     1441 gattttgttt cattgactga gcttgagctc gatttttcgg aagaagatgt ggaatttgcc
     1501 gatcgttccc aattaatggc acttgccaat gaaatagaag aattaattct aaaactatct
     1561 aattcgttca aagttggaaa tgctttaaaa aacggcattc cggtagccat tgttggcgaa
     1621 acaaatgtgg gcaaatctac gctgctaaat gttttgctca atgaagacaa ggctattgtt
     1681 tccgatattc acggaacaac acgagatgcc atcgaagatt cggtgaatat agacggaatt
     1741 acttttcgct ttattgatac agccgggata cgtgaaacca aagatgaaat tgagacactc
     1801 ggcattgcac gaacgtatca aaaaattgaa caagcttcga ttattctatg gttaatagat
     1861 tgtacccaac ttagtgaacg cattgaaacg cttacagaaa aaattgcaaa aaaaattcaa
     1921 gataaaaaat ttatactgat tttcaataaa atagacaaaa ttacagacga agaacgtgaa
     1981 gttttaaatc aattttttga acattattcg ggcgaacgca tttatctttc cgcaaaaaaa
     2041 agaatcaaca ttcatcaatt acaaaaatta ctggtaaaag ctgcccagtt gcccgaggta
     2101 caacccggcg atgtggtggt cagtaacgtc agacattacg aagcactgca aaatgcttta
     2161 aaagccattc agcgagtgaa agaaggcatt caaaacggaa tttccggcga ttttctttca
     2221 caagatttac gcgaatgtat gttttttctt ggagaaatta ccggcgaaat ctccagtgac
     2281 gaaattttgg gcaatatttt cagtaagttt tgtatcggaa agtaaccccc ctaaacaaac
     2341 aaataacaaa acaaacaaaa aacatattaa aatgcatata attagcattt tagtaaaagt
     2401 aaatttattt ttgttgattg ttttttattt gaatttttat catatacttg taccttattt
     2461 gtacctcgaa ctataaaagg tatttttttc actgattttg tacctcaaat ggtttacaaa
     2521 gtttacactt tggcacaatt ttcatcagaa tataatttat tttttaaagg tctgatggaa
     2581 cccacatgtc tgaatattat atttattcgt gtttgtgtat gtaatacatg tgggtttcat
     2641 attaaataac ctctctccta cttttcaagc cgaaaaaatt tacgatacga gcatttgata
     2701 acggctgttt ccagagacgt agcatgctac gtctctactg aacaggttga atgacagatg
     2761 gttattt
//
LOCUS       DAOSIB010000136         2765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_31753_length_2765_cov_10.875277, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000136 DAOSIB010000000
VERSION     DAOSIB010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31753_length_2765_cov_10.875277"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="PLD44_08580"
     CDS             complement(<1..891)
                     /locus_tag="PLD44_08580"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HOF99417.1"
                     /translation="MNFNFKSRFFGFLLLGIPFLILMMLMMNGCNSSKKTSGIDLTNL
                     DTTARPGDDFYQYACGGWMKKNPLKGEYARYGTFDKLAEENTEQLRQLIEEIASQSHA
                     DGTVEQKIGDLYQLAMDSDRLNRDGYLPVLGELKKLSELKTTNSLIQILPELMLSGTD
                     VYFSVYVDADPMNSSVYLTQTYQSGISLGEREYYLDQDAHSKEILEKYKQHVAKMFEL
                     FGFNSAEAQKNRDAVLSIETRLAKASYDRVKLRDPYANYHKMSLQDLGKEYSAISWKE
                     FLTALGIPAVDSVNVSQKEFL"
     gene            complement(930..1952)
                     /gene="dusB"
                     /locus_tag="PLD44_08585"
     CDS             complement(930..1952)
                     /gene="dusB"
                     /locus_tag="PLD44_08585"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOF99418.1"
                     /translation="MKISNIEFPSRPLFLAPMEDVTDADFRVLCRHFGADMVYSEFVS
                     SDALNRNIPRTLQKIKVLEEERPVAIQIYGTDPEVMAESARKVEIIHPDIIDINFGCP
                     VKKVAGKGAGAGMLKDIPKMLKITEAVVKAVHLPVTVKTRLGWDDSSKIIVELAEMLQ
                     DCGIQALTIHGRTRQQMYKGDADWTLIGEVKNNPRIKIPVIGNGDITTPEQAKEYFNR
                     YGVDAIMIGRAAIGQPWIFSEMKKVLDDTFEYSPFSFEDKKAILKNYILSAVRRLDLD
                     EKSSIENMYDMHLKGIVHSRRHLAATPIFKNIPNFKNTRIAMLRADTLNELFEIIDSV
                     SEPSGY"
     gene            2162..>2765
                     /locus_tag="PLD44_08590"
     CDS             2162..>2765
                     /locus_tag="PLD44_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOF99419.1"
                     /translation="MERTINKTNTYPIERNEIEIMAPAGSWESLAAAIKAGADSVYFG
                     MGKLNMRSKSSSNFTTDDLKKIVEICSENKVKSYLTINTILYDNDLTLMRQIIETAKE
                     AGISAIIASDVAALTYANSLNVEVHLSTQLNISNIEALKFYAQFADVVVLARELNLTQ
                     VSEIFRSIREENICGRNRKPVRIEMFCHGALCMAVSGKCYL"
BASE COUNT          943 a          533 c          473 g          816 t
ORIGIN      
        1 caaaaattct ttttgagaaa cattaacaga atcgacagca ggaattccta aagctgttag
       61 aaattctttc caagagatgg cagaatattc tttacctaaa tcctgaagag acattttatg
      121 ataattagca taaggatcac gaagtttaac ccgatcatag gaagctttgg ccaatcgagt
      181 ttcaatactc aaaacagcat cgcgattctt ttgtgcctca gcagaattaa aaccaaacaa
      241 ttcaaacatt ttggctacat gctgcttgta tttttcgaga atttctttag aatgagcgtc
      301 ctgatcaagg taatattctc tttctcctaa actgatacca ctttgatagg tttgcgttag
      361 atacactgaa ctgttcatcg gatcggcatc aacgtaaacc gaaaaataaa catcagtacc
      421 agacaacata agttccggga gaatttgaat cagtgagttt gtagttttta attccgacaa
      481 ctttttcaat tcaccaagca caggcagata accgtcacga tttaatcggt cgctatccat
      541 agccaattgg tacaaatcac cgattttttg ctctaccgta ccgtcagcat gcgattgtga
      601 agcaatttct tcaatcaatt gccgaagttg ttccgtattt tcttcggcta acttgtcgaa
      661 ggttccataa cgtgcatatt caccttttaa agggtttttt ttcatccagc cgccacaggc
      721 atattgataa aaatcatcac cgggccgagc tgtagtgtcc aaattggtca ggtcgatacc
      781 tgaagttttt tttgaagaat tacatccatt catcataagc atcattaaaa ttaaaaatgg
      841 aattcccagc aagagaaaac caaaaaaacg tgatttaaaa ttgaaattca taaactatag
      901 aattttagaa aattaaaata tatgaaaaac taatagccag agggttcact cactgaatca
      961 ataatttcaa aaagttcatt taaagtatcg gcacgtagca tagcaatgcg tgtgttttta
     1021 aaattgggaa tatttttaaa aataggagta gctgccaaat gtcgacggga atgaacaatc
     1081 cctttcaaat gcatgtcata catattttct atggaagatt tttcatcaag atccaatcta
     1141 cgaacggcag ataaaatata gttttttaaa atagccttct tatcttcaaa agaaaatgga
     1201 gaatattcaa aagtatcatc gagaactttt ttcatttcgg agaaaatcca cggttgacca
     1261 atggcagctc tgccaatcat aatggcatcc acaccatacc gattaaaata ttcttttgct
     1321 tgctccggcg tagtaatatc tccattgcca atcaccggaa ttttaattct tggattgttt
     1381 ttaacttcgc caatcaaagt ccagtcagca tctcctttgt acatttgttg acgagtcctt
     1441 ccatgaattg ttagagcctg aataccacaa tcctgcaaca tttctgccaa ctcaacaatt
     1501 attttactgc tatcgtccca gcccaaacgc gttttaacgg taacaggaag atgcacggct
     1561 ttgacaacag cttcagtaat tttcaacatt tttggaatat ctttcaacat ccccgctcct
     1621 gctccttttc ctgcaacttt cttcaccgga caaccaaaat ttatgtcgat gatatcggga
     1681 tgaattattt caacttttcg agccgattca gccataactt ccggatcagt tccataaatc
     1741 tgaatggcta caggcctttc ttcttccaac acttttattt tttgcaaagt acgcggaata
     1801 ttacgattca atgcatccga agagacaaat tcggaataaa ccatgtctgc accgaaatga
     1861 cgacacaaca cacgaaaatc ggcatcggta acatcttcca tcggtgctaa aaacaaaggt
     1921 cgggatggaa attcaatatt tgaaattttc atttttatgc aaaaaagagt acaaatttag
     1981 aaaaaaattt ttaatggcaa tttcaatgaa aatttatacg ttatacttcg ggaaaaacaa
     2041 aaactcgaat catttgttca aaataaaaca agatgaaaaa aaaagtagga tttgattgaa
     2101 taaaataaat aaaaataaaa gggttaattt tgcaaaataa aattgctcag aaaatcgaat
     2161 aatggaaaga actataaaca aaaccaatac ctatcctatc gaacggaacg aaatagaaat
     2221 tatggctcct gcaggctcgt gggaaagctt ggcagcagcc ataaaagccg gagccgactc
     2281 tgtatatttc ggtatgggaa aattaaatat gcgcagtaaa tcgagcagta attttacaac
     2341 tgacgatcta aaaaaaattg tggaaatatg ctccgaaaac aaagtaaaaa gttatttgac
     2401 cattaataca attttatacg acaacgattt gactttgatg cgccaaataa tcgaaactgc
     2461 aaaagaagcc ggcatctctg ccataattgc ttcggatgtg gcagcactga cttatgccaa
     2521 cagcctgaat gttgaagtac atttgtcgac tcagctaaat atttccaaca ttgaagcatt
     2581 gaaattttat gcacaatttg ctgatgttgt ggtgttggcc agagaattaa acttgacaca
     2641 ggtttcagaa atttttcgct ccattcgaga ggaaaatatt tgtgggcgaa acagaaaacc
     2701 ggttcgtatc gaaatgtttt gtcatggagc gctttgcatg gccgtatcag gaaaatgcta
     2761 tctca
//
LOCUS       DAOSIB010000137         2764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_31769_length_2764_cov_10.354743, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000137 DAOSIB010000000
VERSION     DAOSIB010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31769_length_2764_cov_10.354743"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PLD44_08595"
     CDS             complement(<1..586)
                     /locus_tag="PLD44_08595"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOF99420.1"
                     /translation="MENNLPKGWVECSIGEILTAKKGKKPASTIDEPREGYVPYILID
                     EMEGKQIRTYTNDPKVSIIDETEVLLVWDGSIGKCGSGLKGAIGSTLVGLKALGGIQT
                     KFLEYTIRQQNNFIKETSTGTGLQHINKDFFEICKIPLPPLAEQHRIVAKLDALFEKI
                     ESNKQRLEKIPKLLKRFRQSVLAAAVSGKLTEEWR"
     gene            complement(576..1037)
                     /locus_tag="PLD44_08600"
     CDS             complement(576..1037)
                     /locus_tag="PLD44_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOF99421.1"
                     /translation="MYILECSNGMYYTGSTNNLELRLQQHQNGEGANFTKKHLPVKLV
                     YFEEFARVDEAFYREKQIQGWSRKKKEALINNQFEKLHDFAECKNETHFLNVPFDSAQ
                     GTENNSAQGTGNDSAQGTGNNSAQGTAPTRLLSGAETTDSLTLLKSNGNGE"
     gene            complement(1049..2449)
                     /locus_tag="PLD44_08605"
     CDS             complement(1049..2449)
                     /locus_tag="PLD44_08605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOF99422.1"
                     /translation="MSDVVNKLWGFCHTLRHEGIDYNDYIEQLSFLLFLKMADEKEVH
                     LNDKYNWKALKELSGGDLMDHYTDSLKELAKEPGLLGEIFTQAQNRFNNPVSLKKLLN
                     LIDEDEWTSMDIDVKGAAFEGLLEKAASEGKKGAGQYFTPRPLIQSMVRLMKPDPRGK
                     NTFTIYDPACGTGGFLVCAYEWLLQECKGAFPREDVKRIRENTYYGQELVARPRRLAL
                     MNMYLHGVTPKIKLGDTIYNNPSSDRFDVILANPPFGTKGANQIPERDDFTVETSNKQ
                     LNFVQHIMSTLKIGGRAAIVLPDNVLFEDKAGEVFEILMQECNLHTILRLPRGTFTPY
                     AQGVKANVIFFQKGLPTEKVWIYDNRSNIESITKKDRPLTAKHFEDFEKCYGSDPNGN
                     SPRTDQGETGRFRCFTIDEIKKRNYKLDITWLKDESIEDADSLPEPNDLATEAIAELE
                     AVVDDLKEIISLLEKE"
     gene            complement(2442..>2764)
                     /locus_tag="PLD44_08610"
     CDS             complement(2442..>2764)
                     /locus_tag="PLD44_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004351667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HOF99423.1"
                     /translation="MGTIQLKKKLGVPDSRPVADFLPTISIKAKDLAAEMTGLNVQNK
                     DLKGQSLIEKEHVDNNLAVREMLTKRGIFPENLPPAEDVKKLQRKLEGEEKKILTKKR
                     RNNE"
BASE COUNT          693 a          597 c          488 g          986 t
ORIGIN      
        1 ccctccactc ctccgtcaac ttaccactta ccgcagccgc taaaactgat tgtcggaagc
       61 gttttaatag ttttggtatt ttttctaatc gttgtttgtt gctttctatt ttttcaaata
      121 aagcatcaag tttggctaca atgcgatgtt gctcagcgag aggggggagt gggattttgc
      181 atatttcaaa aaagtcttta ttgatatgtt gcaacccagt tcctgtgctt gtttctttaa
      241 tgaaattatt ttgttgtctg attgtatatt caagaaattt tgtttgaata cctcctaaag
      301 cctttaagcc aactaaagta gaacctattg cccctttcaa cccactacca catttaccaa
      361 tgctgccatc ccaaactaat aaaacttctg tttcgtcaat tattgatact ttagggtcat
      421 ttgtatatgt tctaatttgt tttccttcca tttcatcaat aagaatgtaa ggaacgtaac
      481 cttctctcgg ttcgtcaatt gttgaagccg gtttcttgcc ttttttggca gtcagaattt
      541 caccaatact gcattcaacc caaccttttg gcaaattatt ctccatttcc attgcttttc
      601 aaaagggtta atgaatcggt tgtttcggct ccgctcaaca accgggtagg tgccgtaccc
      661 tgagcggagt tatttcccgt accctgagcg gagtcatttc cagtaccctg agcggagtta
      721 ttttccgtac cctgagcgga gtcgaagggt acattcaaaa aatgtgtctc atttttacat
      781 tcagcaaaat catgaagttt ctcaaattga ttattgatca atgcttcctt tttcttcctg
      841 ctccagcctt gaatttgttt ctctctataa aaagcttcat caactcttgc aaattcttca
      901 aaataaacta acttaacagg taaatgcttt ttagtaaaat tagcaccttc tccattttga
      961 tgttgttgca atcgtaattc taaattatta gtgctgccgg tataatacat gccgttgctg
     1021 cattctaaaa tatacatcca ccctttcatt attctttttc taataaacta attatttctt
     1081 ttaagtcatc cactacagct tccagttcgg caatggcttc ggtagcaagg tcgttgggtt
     1141 cgggcaggct gtcggcatct tcaatgcttt cgtcttttag ccatgtgata tcaagtttgt
     1201 aattacgttt tttaatttca tcaatggtaa agcaacggaa acgccctgtt tctccttgat
     1261 cagttcgtgg gctgttgcca ttggggtcag agccatagca tttttcaaaa tcttcgaaat
     1321 gtttggctgt tagcgggcgg tcttttttgg taatgctttc tatattgctg cggttgtcgt
     1381 aaatccagac tttttcggta ggcaatcctt tctgaaagaa aataacattt gcttttacgc
     1441 cttgtgcata aggtgtaaat gtacctcttg gcaagcgaag aatggtgtgc aagttgcatt
     1501 cctgcatcag tatttcaaaa acttcccctg ctttatcttc aaacaataca ttgtcgggta
     1561 aaactatggc ggcacgtccg cctattttga gcgtactcat gatgtgttgc acaaaattga
     1621 gctgtttgtt gctggtttct accgtaaaat cgtcacgctc cggtatttga tttgctccct
     1681 ttgtgccaaa tgggggattt gccaaaatca catcaaaacg gtcgctactt gggttattat
     1741 aaatggtatc gcctaatttt attttaggag taacgccatg caaatacata ttcatcaaag
     1801 ccaatcgtct tggacgggca accaattctt gtccgtaata agtgttttct ctgattcgtt
     1861 tcacatcttc acgaggaaaa gcacctttac attcctgtaa aagccattca taagcacaaa
     1921 ccaaaaaacc acctgtaccg caagcggggt cataaatggt aaatgtattc tttcctcttg
     1981 gatcgggctt catcaagcga accatgcttt gtattaaagg acgcggtgta aaatattgtc
     2041 cagcaccttt tttcccttcg ctggctgcct tttccagcag tccttcaaag gcagcacctt
     2101 tcacgtctat gtccatcgaa gtccattcat cttcatcaat caggttgagc agttttttga
     2161 gacttaccgg attgttaaaa cggttttgtg cctgcgtaaa gatttcgcca agtagtcccg
     2221 gttctttagc cagttctttt aacgagtcgg tatagtggtc cattaaatcg ccaccgctta
     2281 attctttcag tgctttccaa ttgtatttgt cgttcagatg gacttctttt tcatcagcca
     2341 tttttaagaa aagaagaaaa gaaagttgtt cgatataatc gttgtaatca attccttcgt
     2401 gtcttaatgt atggcaaaag ccccacaatt tatttaccac atcactcatt atttcttctt
     2461 ttttttgtca gtattttttt ctcttcgcct tccaatttac gttgcaactt tttaacatct
     2521 tcagcaggtg gaagattctc gggaaatatt cctcttttcg ttaacatttc acggacagca
     2581 aggttattgt caacatgttc tttttctatt aaactttgtc cttttaaatc tttattttgc
     2641 acattcaacc ctgtcatttc agcggccaaa tcttttgcct ttatgcttat ggtcggtaaa
     2701 aaatctgcaa cgggacgact atcaggtacg cctaatttct ttttcagttg tattgttccc
     2761 atcc
//
LOCUS       DAOSIB010000138         2681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_33070_length_2681_cov_12.979817, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000138 DAOSIB010000000
VERSION     DAOSIB010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33070_length_2681_cov_12.979817"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..>2681)
                     /locus_tag="PLD44_08615"
     CDS             complement(6..>2681)
                     /locus_tag="PLD44_08615"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOF99424.1"
                     /translation="MKIHFKIHFITQWGQSIVLLVKNDNHDTPQRIEMNCGKNFEWTA
                     VIDIHSDTKELFYRYALENDSSTLEFEYGDFRQIKIDTEFSDVYISDNWRARYGDSPF
                     ESSAFTDCLFKRNSLYAAPQVKDANLVLRLRCAQLEPNRHFAVIGNQDALGNWDVAKK
                     IRLDESDFPVCSVALNATKFKFPLEYKYLIVDTATDEVLAWGGGPNRVVESVMKKSLN
                     IVNDEHFIRTIPSWKGAGVAIPVFSLRSEESCGIGEFYDLKKMVDWAKKTGQRIIQTL
                     PINDTILYHTNYDSYPYNAVSVYALHPIYLHLESMGTLNDELQKKYFEDKKKEFNAKT
                     FSDYQNVMNLKWEYFEAIYQQEKATVFASESYLNFFVSNKSWLIPYAAFSFLRDFYGT
                     PDFRRWEKYSRYEEQEILEFSDPKQPHYDQLAIYYYLQYHLHCQLSEVRQWAHENGVV
                     IKGDIPIGVSPFSVDVWINPELFNTQVQAGAPPDDFSLTGQNWGFPTYNWEEMEKDDF
                     LWWKKRFQKLSEYFDAYRIDHILGFFRIWEIPADDVWGLTGSFHPALPFTRQELEAKG
                     LRWDESRYLEPYLKEHVLYATFGKYADDVIREFFVPHGWKSYKFKPEYDTQKKVEAYF
                     NSLGYNFSNKDVMIRDGLYSLHCEVLFVRDLRQPEKFHPRIAMHSTASFRDLPDETRR
                     TLDRIYVDFFYHRHNEFWKQQAMKKLPALITATSMLPCGEDLGMVPDSVPEVMQILEI
                     LSLEVQRMPKKPNMEFGMPSEAPYLSVCTTSTHDMNPLRAWWEEDSAVTQRFYNHALG
                     LQGAAPKTCETWIVERIVQQHLQSKAMWVILPWQDWMALDEKLRYEHPFAERINVPSN
                     PRNFWCYRMHLTLEKLLSEDDFNQYLFQLISQSGR"
BASE COUNT          827 a          585 c          483 g          786 t
ORIGIN      
        1 atttcttatc ttcccgattg gctaatcagt tgaaataaat attgattgaa atcatcttca
       61 ctcagcaatt tttcgagtgt caaatgcatt cggtaacacc aaaaatttcg tggattgctg
      121 ggtacgttga tgcgttcagc aaaaggatgt tcgtagcgaa gtttttcgtc caaagccatc
      181 caatcctgcc aaggcaaaat tacccacata gctttcgatt gaagatgctg ttgaacaatg
      241 cgttcaacaa tccatgtttc acaagttttt ggagctgctc cctgcaggcc cagcgcatga
      301 ttgtaaaaac gttgagttac ggcggaatct tcctcccacc atgctctgag tggattcata
      361 tcatgagtag aagtggtaca caccgataaa tagggagctt ccgatggcat tccaaattcc
      421 atattgggtt tttttggcat gcgttgaact tcaagactca aaatttcgag tatttgcatt
      481 acttcgggca cagaatcggg caccataccc aaatcctctc cacacggtaa catactggta
      541 gcagtaataa gggctggtaa tttcttcatt gcctgctgtt tccaaaattc gttatgacga
      601 tgatagaaaa aatcgacgta aattctatcc agcgttcgac gcgtttcatc cggcaaatcg
      661 cgaaaggaag cagtactgtg catggcaata cgcggatgaa atttttccgg ttgacgtaaa
      721 tcgcgcacaa aaagaacttc gcaatgcaaa gagtataaac cgtcgcgaat catcacatct
      781 ttattgctaa aattatatcc caaagaattg aaataagctt caactttttt ctgcgtatca
      841 tattcaggtt tgaacttata acttttccag ccatgaggta caaaaaattc gcgaatgaca
      901 tcatcggcat attttccaaa agtggcatac aacacatgtt ctttcaaata aggttccaag
      961 taacgacttt cgtcccacct aagtccctta gcttcaagtt cctgacgcgt aaaaggcaaa
     1021 gcaggatgaa aactgccggt aagtccccag acgtcatcag ccggaatttc ccagatacga
     1081 aaaaatccga gaatatgatc gatacgataa gcatcgaaat actccgaaag tttttggaaa
     1141 cgctttttcc accaaagaaa atcatctttt tccatttctt cccaattgta agtgggaaaa
     1201 ccccaatttt gacccgtaag tgaaaaatca tcaggtggag caccggcctg tacctgcgta
     1261 ttaaacaatt ccggattaat ccacacatcc acactgaaag ggctcactcc aataggaata
     1321 tcacctttga taactacacc attttcatgt gcccattgcc ttacttcgga caactgacaa
     1381 tgcaaatgat attgcaaata ataatatatg gcaagttgat cataatgtgg ctgttttgga
     1441 tcggaaaact ccaatatttc ctgttcttcg taccggctgt atttctccca tcgtctgaaa
     1501 tcgggcgttc cataaaaatc acgtaaaaaa gaaaatgcag cataaggaat caaccaagat
     1561 ttattggaga caaaaaaatt caaataggat tcggaagcaa agactgtggc tttttcctgt
     1621 tgataaatag cttcaaaata ctcccattta agattcatca cattttgata atcggaaaaa
     1681 gttttggcat taaattcttt tttcttatcc tcaaaatatt ttttctgcaa ttcatcattt
     1741 aaggttccca tactttccag atgaagatat atgggatgga gcgcataaac cgaaacagca
     1801 ttgtaaggat acgaatcgta attggtatga taaagtatgg tatcgttaat tggcaaagtt
     1861 tgtatgatac gctgaccggt tttttttgcc caatctacca ttttttttaa atcgtaaaat
     1921 tcacctattc cgcaactttc ctcactgcgc agcgaaaaaa caggtatggc cacgccggca
     1981 cctttccatg aaggtatcgt acgaataaaa tgttcatcat taacgatatt tagcgatttc
     2041 ttcatcaccg attccaccac cctgtttggt ccgccacccc atgccagcac ttcatccgta
     2101 gccgtatcaa ctattaaata tttatattcg agcggaaatt tgaattttgt ggcattcaac
     2161 gcaacgctgc acaccggaaa atcagactcg tccagacgaa ttttctttgc aacatcccag
     2221 ttccccaatg catcctgatt gccaataaca gcaaaatgcc gatttggttc aagctgagca
     2281 catcgcagac gaagaaccag atttgcatct ttgacctgtg gggctgcata caatgaattt
     2341 cttttgaaaa gacaatcggt aaaagccgaa gattcaaacg gactatcgcc gtaacgtgct
     2401 cgccaattat cgctgatgta tacgtcggaa aattccgtgt cgatttttat ttgacgaaaa
     2461 tcgccatatt caaattccaa tgtagatgaa tcgttttcca aagcatagcg gtaaaaaagt
     2521 tcctttgtat ccgaatgaat atcaataacg gctgtccatt caaaattttt accgcaattc
     2581 atttctattc tttgcggtgt atcatgattg tcgttcttta ccaaaagaac aatcgattgc
     2641 ccccactgcg taatgaaatg tattttaaaa tgaattttca t
//
LOCUS       DAOSIB010000139         2594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_34528_length_2594_cov_10.629672, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000139 DAOSIB010000000
VERSION     DAOSIB010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34528_length_2594_cov_10.629672"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..834)
                     /locus_tag="PLD44_08620"
     CDS             complement(25..834)
                     /locus_tag="PLD44_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99425.1"
                     /translation="MSIFQTIVNKYKDAKQKSLNKKDFKKALLQAVNDGKITKEEIDE
                     LDKKKSEFGLTDEDVKGMRAEIFAAAFSATKEDQQVTKDEEQELEKIQKFLGLADDEI
                     QHNKKELARLRLLNEIQQGNMPTVQVTNLIMQKGEKAYWIEPATLAEEKVLRRKYEGG
                     SQGMSFRVMKGVSYRVGGHRGHITSETGVVAVSSGELIITSKRAVFRGDNKSFAVKLD
                     KILDIQLFTNGLQFSENNRSKPRLIKFAQQGNHDIIGAVLSYAINHYGDKE"
     gene            complement(871..1551)
                     /locus_tag="PLD44_08625"
     CDS             complement(871..1551)
                     /locus_tag="PLD44_08625"
                     /inference="COORDINATES: protein motif:HMM:NF019191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HOF99426.1"
                     /translation="MDNLFLLLFLASFVCLIIGLIKPTIFSRFIKGEITRKKIGLIFG
                     IATVAFFVMFGMTTDTSKQTQSNTNEKVAEQKTESKQLSPEEQAKKDADDALAKKQAD
                     EEKAKQEAEAKAQKEAEEAAKNVPAEYKSALNKAASYANTMHMSKRGVYDQLVSEYGE
                     KFSAAAAQYAIDNVKADWNANALAKAKNYQNTMNMSPAAIRDQLTSEYGEKFTKAEAD
                     YAIQHLND"
     gene            complement(1582..2220)
                     /locus_tag="PLD44_08630"
     CDS             complement(1582..2220)
                     /locus_tag="PLD44_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99427.1"
                     /translation="MIFGKIFNSNNDPEGYIGYFNLKDWWLNTFTEEERNHMVAIFQP
                     MGASKDTLIKGKFEMLSNNSPLSFLTSLTGWFDNPRDRNLAHKIISKAEDFVGTEKDI
                     LNLHFFYPTKMKLFYKNRDSDPKSLQISIDSALKQIEIAEKAAAEFKKEYPDSSLPAH
                     EGYSQFCIILEKQGKFNEAVKYAKQAKDQGWNGDWDNRIAKCQKKIEKMQQK"
     gene            complement(2224..2421)
                     /locus_tag="PLD44_08635"
     CDS             complement(2224..2421)
                     /locus_tag="PLD44_08635"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOF99428.1"
                     /translation="MSNITKTLRKLREAKGLSQEKLARLADVANNTIIKIEAGKNQNP
                     TLDTLKKISKALDVSVDELIK"
BASE COUNT          676 a          503 c          431 g          984 t
ORIGIN      
        1 gccagttgaa aactggcgcg ccgcctattc tttgtctccg taatgattga ttgcgtaaga
       61 aagaacggct ccaattatat cgtgatttcc ttgttgtgca aactttatca atcttggttt
      121 tgaacgattg ttttccgaaa attgtaatcc atttgtaaaa agctgaatat ctaaaatttt
      181 atccaatttg acggcaaaag atttattatc gcctcggaaa acagctcgct tgctggtaat
      241 gattagttcg ccactactta cagcaacaac tcctgtttcg ctagttatat gcccacggtg
      301 tccgcctact ctgtagctga cacctttcat aactcggaaa ctcattcctt gtgaaccgcc
      361 ttcatatttt ctgcgtaaaa ctttttcttc ggctaatgta gctggttcaa tccaatatgc
      421 tttttcgcct ttttgcatta tcaaattcgt tacttgtacc gtgggcatat tgccttgctg
      481 gatttcgttc aaaagacgca agcgagcaag ttcttttttg ttgtgctgaa tttcgtcatc
      541 cgccaaacca agaaattttt gaattttttc aagttcctgc tcttcatctt tcgtaacttg
      601 ttgatcttct ttggtagctg aaaaagccgc cgcaaaaatc tcagctctca ttccttttac
      661 atcttcatca gttaagccaa actcgctttt ctttttatca agttcatcga tttcttcttt
      721 ggtaattttt ccgtcattga cagcttgaag caacgccttc ttaaaatctt ttttattaag
      781 agatttttgt ttggcgtctt tatacttatt tacgattgtt tgaaaaatgc tcataacttt
      841 tatatttaca tcttgaaggt ttgaaaatta ctaatcatta aggtgttgaa tagcatagtc
      901 tgcttctgct ttagtaaatt tttcaccata ttcagaagtt aattgatcac gaatagcagc
      961 tggcgacata ttcatagtgt tctgatagtt ttttgctttg gccagcgcat ttgcgttcca
     1021 gtcggctttt acattgtcta ttgcatactg tgcggcggct gctgaaaatt tctcgccata
     1081 ctctgaaact aactggtcgt aaacacccct cttggacatg tgcatcgtgt tggcgtaaga
     1141 agcggctttg ttaagggcgg acttatactc ggctggcaca tttttcgctg cttcctcggc
     1201 ctctttttgt gcttttgcct cagcttcttg ctttgctttt tcttcatctg cctgtttctt
     1261 ggcgagagca tcgtctgcgt cttttttggc ttgttcctct gggcttaatt gttttgattc
     1321 tgttttttgt tctgccactt tttcgttagt gtttgattgt gtttgtttgc tagtgtcagt
     1381 tgtcattcca aacatgacaa aaaaggcaac ggtggcaata ccaaaaatta aacctatttt
     1441 ctttcttgtg atttcgcctt taataaagcg agaaaaaatc gttggtttga ttaacccgat
     1501 tattaaacag acaaacgacg cgagaaataa aagtaaaaat aagttatcca tagttttgtt
     1561 cctttaattt attaaattat attatttttg ttgcattttt tcaatttttt tctggcattt
     1621 tgcaatacga ttatcccaat ctccattcca accttgatcc ttagcttgtt tggcatattt
     1681 tacagcttca ttaaattttc cctgtttctc taaaattatg caaaattgag aatatccctc
     1741 gtgagctggc agtgagctat caggatattc tttcttaaat tccgccgctg ccttttctgc
     1801 aatttctatt tgttttaagg cagagtcaat tgaaatttgt aatgactttg gatcgctatc
     1861 tctattctta taaaataatt tcattttagt tggatagaaa aaatgcaagt taagtatatc
     1921 tttttccgtt ccaacaaaat cttccgcttt tgaaattatt ttatgtgcga gatttctgtc
     1981 tctcggatta tcaaaccaac cagtcaaaga agtaagaaat gacaatgggc tattattgct
     2041 caacatttca aactttcctt ttatcagagt atcttttgat gcacccatcg gctgaaaaat
     2101 agcaaccata tgatttctct cttcttctgt aaaggtattc aaccaccaat ctttcagatt
     2161 aaaatatcca atatatcctt cggggtcatt atttgaatta aatattttcc caaaaatcat
     2221 aatctacttt attaattcat caacactaac atctaatgct ttcgatatct tcttaagtgt
     2281 gtctagtgtt ggattttggt ttttgccagc ttcaattttg ataattgtat tattagcaac
     2341 atcagccaat ctcgctaatt tctcttgaga taatcctttt gcttctctaa gttttctaag
     2401 tgtttttgta atgtttgaca tactaatatc gtagtagtag aattaaagta tcagtaatat
     2461 taactttcaa ctactttaat gctacaccaa aaattaaaaa tagtaaagaa tgagggagta
     2521 aagctgttgt gtgcgcacgg gtgggcgggg caaatacaac tttcttattg gcggcgcatt
     2581 tcgcaaagcg aaat
//
LOCUS       DAOSIB010000140         2580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_34755_length_2580_cov_9.497426, whole genome shotgun sequence.
ACCESSION   DAOSIB010000140 DAOSIB010000000
VERSION     DAOSIB010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34755_length_2580_cov_9.497426"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLD44_08640"
     CDS             <1..215
                     /locus_tag="PLD44_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99429.1"
                     /translation="GDSEPSHRPACIVWCSEKFEKKYPDKVEALRMNKNKYYRSNFVF
                     HSALDAANIQSKVMDKKLSIFNLPAQ"
     gene            215..1372
                     /locus_tag="PLD44_08645"
     CDS             215..1372
                     /locus_tag="PLD44_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99430.1"
                     /translation="MEKELIHKQSSLSFLLLNKYVWAACIVLGLIVKAYFLPVKTGDY
                     VVYLKPWIDFIKTHGYFSALKYDFSNYTPAYLYFLTLVAKIPIEPVFAIKIFSIAFEF
                     LAAYFLGKIVCLKYHNSLIILISLAIIPWLPTVMLNSSYLSQCDAIYASFVMGSIFYA
                     LKKRPFLSVLFLGIAFAFKMQAVFVLPFFFVFLLRKEIKWYYFFIVPAVYLVSILPAW
                     FNGRSFVELISLYFSQADYYRLLTMNFPNTYIWFSGADFDTFSLAGILFTVLLTLVAG
                     FWLRTPKITFTLETWIKFLFLSFIVVPFVLPGMHERYMYLGDIFGLAYLMFFPKKWYL
                     PFGILLVSTYSYIRCSRFSEVLPMQPAFFLYLLVILLTFYDFVESLKFSRK"
     gene            1369..>2580
                     /locus_tag="PLD44_08650"
     CDS             1369..>2580
                     /locus_tag="PLD44_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99431.1"
                     /translation="MNAVHKVNFTFLGVIIFVYVLLTAGCLNNCYFWDVVQQVSKEAH
                     WYYQTNFKYLIIPSQNSLSDIVATGYHPPLMGMLTAALWKIFGVHLWISHLLTLICFF
                     VLLYQVIKLVEKFFPAHQAGWVMLVVFMEASLLAQFSIASPDFILLTSFIASLRGLLE
                     RNTWLSSIALIFLFGINMRGIFAGIALWMSFQLFLYLNSEKKFSFTLLAASIRPFLPA
                     IILSLIYFIFYFRMNGWFSGVSEEGGHYSLPENISMIGKHFFEFLLRSVENGRIIVWV
                     FSLFAFYWIAKKKVPISNELKMLICCFLMLFSIYFLFVFITRMPFSTRYFMPHFFLLT
                     LITLSVFSFRLPETKWKATVLVFLLFELTGHLWIYPEKMAKSWDCTLAHLPFYELRKQ
                     CFDYIDRQNMDY"
BASE COUNT          622 a          440 c          498 g         1020 t
ORIGIN      
        1 caggtgatag tgaaccttct catcgtccgg cctgtattgt ttggtgttcc gaaaaatttg
       61 aaaaaaaata tcccgataag gtggaagcgt tgagaatgaa caaaaacaaa tattatcgca
      121 gtaattttgt ttttcacagt gctttggatg cagcgaatat tcaaagtaaa gtgatggata
      181 aaaagcttag tattttcaat ttgccggctc aataatggaa aaggaattaa ttcataaaca
      241 atcatcgttg tctttcttat tgctgaacaa atatgtttgg gcagcatgta ttgtgctggg
      301 gttaattgtg aaggcgtatt tcttacccgt aaaaaccggc gattacgtgg tgtatctcaa
      361 accctggatt gatttcataa aaactcatgg ttatttttca gctttaaaat atgatttttc
      421 caattatacg ccggcttatc tttattttct gacgcttgtt gccaaaatcc caattgaacc
      481 tgtttttgcc attaaaattt tttccatagc ttttgaattt ctggctgctt attttttggg
      541 aaaaattgtt tgtttgaaat atcacaattc tttgattatc ttgatttcat tggctattat
      601 cccctggtta ccaaccgtga tgctcaacag tagttatttg tctcaatgtg atgccattta
      661 tgcttctttt gtgatgggca gcatctttta tgctttaaag aagcgaccat ttttatcggt
      721 gctttttctg ggaatagctt ttgcatttaa aatgcaggct gtttttgtat tgcctttctt
      781 ctttgttttt cttttgagaa aagaaattaa atggtattat ttttttattg ttcctgccgt
      841 ttatttggta agcattttac cggcatggtt caatggccga agttttgtcg aattaatttc
      901 tctgtatttt tctcaagccg attactatcg gttgttgacc atgaattttc ctaatactta
      961 tatctggttt tcaggagcag attttgatac tttttcatta gccggcatat tatttaccgt
     1021 tttgctgact ttggtggcgg gcttttggct gcgcactcca aaaataactt tcaccctcga
     1081 aacatggata aaatttctat ttctaagttt cattgtggtt ccgtttgttt tgcccggtat
     1141 gcacgagcgc tacatgtatc tgggagatat tttcgggttg gcgtatttga tgtttttccc
     1201 caaaaaatgg tacttgccgt tcggaatttt attggtttcc acttattcgt atatccgatg
     1261 ttcgcgcttt agtgaagttt tacccatgca accggctttt ttcttatatt tgttggtgat
     1321 tttacttact ttttacgact ttgttgaaag tttaaaattt tcccgaaaat gaatgctgtt
     1381 cataaagtta actttacttt tttaggcgtc atcatttttg tttatgtctt attgacagcc
     1441 ggatgtttga ataattgtta tttttgggat gttgtccagc aggtctcaaa ggaagcccat
     1501 tggtattatc aaacgaattt caaatactta atcattcctt cccaaaattc tttgtccgat
     1561 attgtagcaa caggttatca tccgcctttg atgggaatgt tgacggcagc gttgtggaaa
     1621 atttttggtg tgcatctttg gatttctcat ttgctgacat tgatttgttt ttttgtgttg
     1681 ctttatcagg taataaagct ggtcgaaaag tttttccctg ctcatcaggc tggatgggtc
     1741 atgctggtcg tttttatgga agcttcactt ttggcacaat tttccatagc ttcacccgat
     1801 tttattttgt taacttcttt catagcttcg ctcaggggat tattagagag aaatacatgg
     1861 ctttcgtcca ttgccttgat ttttcttttc ggaatcaaca tgcgcggaat ttttgcaggt
     1921 attgctttat ggatgagttt tcagcttttt ctttatctca attcggagaa aaaattcagt
     1981 ttcacattgt tggctgcatc gattcggcca tttttgccgg ctattatttt atcactgatc
     2041 tattttattt tttactttcg gatgaatggc tggttttccg gagtttctga agagggagga
     2101 cattattcgt tgcccgaaaa tatttccatg atagggaaac atttttttga atttttgctt
     2161 cgttcggttg aaaacgggcg catcatcgtg tgggtgtttt cgctattcgc tttttattgg
     2221 attgccaaaa agaaagttcc aatttccaac gaattgaaaa tgttgatttg ctgttttctg
     2281 atgctcttca gtatctactt tctgttcgtt tttatcacca gaatgccttt ttcgacaaga
     2341 tatttcatgc ctcatttttt cctgttgacg ctgattaccc tttcggtatt ttctttcagg
     2401 ttgccagaaa ccaaatggaa agcaacagta cttgtttttc ttttgtttga attgaccggc
     2461 catttatgga tttatcccga aaaaatggca aaatcgtggg attgtacgtt ggcacattta
     2521 cccttttacg aattaagaaa gcagtgtttt gattacatcg accggcaaaa catggattat
//
LOCUS       DAOSIB010000141         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_39222_length_2350_cov_9.320697, whole genome shotgun sequence.
ACCESSION   DAOSIB010000141 DAOSIB010000000
VERSION     DAOSIB010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39222_length_2350_cov_9.320697"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..873)
                     /locus_tag="PLD44_08655"
     CDS             complement(91..873)
                     /locus_tag="PLD44_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF99432.1"
                     /translation="MQFIQTIYTIWQREIIRYWRDKTRIVSTFFMPLMFLVIFGAGLN
                     ETLATGNFGVDFTQFMYPGIIAMSIMNIAFFSTISTVWDREFGFLKEILVAPVSRVAV
                     AFGKTLGATTIASIQAIILLILAPLIGITIHITIIPQLILFMLLLAFAISGMGLLISS
                     LMKTTESFGMVTQILIFPMFFISGAFFPLTSVPSWMSVLSYINPLTYGVDAIRQIILG
                     NQVASNILNNLSLHPISVDALFLVAFSAVMVSAAVFAFNKRA"
     gene            complement(885..1859)
                     /locus_tag="PLD44_08660"
     CDS             complement(885..1859)
                     /locus_tag="PLD44_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF99433.1"
                     /translation="MNIIRVENLTKQFNGFTVVDHINFEVKKGEIFGFLGPNGAGKST
                     TINMLSTILPVTDGEAFINDFSILSQRDNVRKSIGLVFQDPSLDDRLTAEENLRFHAK
                     LYGVPSDEFQKRKQEVLELVDLWDRQNDIVKNFSGGMKRRLEIARGLLHYPAVLFLDE
                     PTIGLDPQTRAHLWDYILRLKREKEMTIFMTTHYMNEAEYCDHIAIIDHGKIVALGTP
                     RELKKQIGGDIISMTSNRKAELKKEIEKRYKKDVKEKDGFLQIEVEDGEKFLPRLFNE
                     LTVEIDSIELRKPTLDDVFLSLTGRAIREEEPSDKERMRQRMQMRRRN"
     gene            complement(1972..2223)
                     /locus_tag="PLD44_08665"
     CDS             complement(1972..2223)
                     /locus_tag="PLD44_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOF99434.1"
                     /translation="MKKSENQRQFYGTVNIGEKGQMVIPAEARKSMGIKKGDKLLVFG
                     MGCDMLAITKLSKVEQFASHLSNQLDIIRKITEKINKKY"
BASE COUNT          718 a          446 c          447 g          739 t
ORIGIN      
        1 cttttttggt aagcaggccc aatgtttgga atattctcaa ccggagtttt catattacac
       61 aattttagca aaaaatactg attggtgcta ctatgctctt ttgttaaaag cgaatacagc
      121 ggcgcttacc attactgctg aaaatgctac aagaaagaga gcgtctacag aaataggatg
      181 tagagaaaga ttgtttaaaa tattacttgc tacttgattg cctaaaataa tttgtcgtat
      241 ggcatctacg ccgtaggtta atggatttat atacgaaagc acactcatcc atgatggcac
      301 agaagttaaa gggaaaaagg cacctgaaat aaaaaacatg gggaaaatta aaatttgcgt
      361 aaccattcca aagctttcgg ttgttttcat aagagaagaa atcagtagtc ccatgccgga
      421 aatggcaaaa gcaagcagaa gcataaaaag aatgagttga ggaataatgg taatatgaat
      481 tgttataccg ataagtggag caagaatcaa taagataatc gcctgaattg aagcaatggt
      541 agttgctcca agtgttttgc cgaaagcaac cgctacccgc gaaacgggcg ctaccaagat
      601 ttctttcaag aaaccaaact ctctatccca gacagtagag atggtggaga aaaaagcaat
      661 gttcataatg ctcatcgcga taataccggg atacataaat tgggtgaaat caacaccgaa
      721 atttcctgtt gcaagagttt cattcaagcc ggcaccgaag ataacaagaa acattaacgg
      781 cataaaaaac gtggaaacaa ttcgcgtttt atctcgccaa tagcgaatta tttctctttg
      841 ccaaattgta tatattgttt ggataaattg cataaatata gattttaatt tcttcgtctc
      901 atttgcatac gctgacgcat gcgctcttta tcggatggtt cttcttccct aatggcgcgg
      961 ccagtgagcg agagaaatac atcgtcgagc gttggcttac gaagttcaat ggaatcaatc
     1021 tcaactgtaa gttcattgaa tagacgtggc aaaaattttt ctccgtcttc aacttctatt
     1081 tgtaagaatc cgtctttttc tttcacgtcc tttttatatc gtttctcaat ttcctttttg
     1141 agttccgcct ttcgattgct cgtcatactg ataatgtctc ctccaatttg ttttttgagt
     1201 tcgcgcggtg tgcccaacgc tacaatcttt ccatggtcaa taatggcaat gtggtcgcaa
     1261 tattctgctt cattcatata atgtgtggtc ataaaaatgg tcatttcttt ttcgcgctta
     1321 agtcgtagaa tataatccca caaatgggcg cgtgtttgcg ggtcaagccc aattgtcggt
     1381 tcgtctaaaa aaagaactgc cggataatgt aggagtccgc gtgcgatttc taatcgccgt
     1441 ttcataccac cagaaaaatt tttaacgatg tcgttttgtc tatcccacaa atcaactaac
     1501 tccaaaactt cctgtttccg tttttgaaat tcatcacttg gcacgccata gagcttggca
     1561 tgaaagcgga gattttcctc tgcggtgagc cggtcgtcca atgacgggtc ttggaaaacg
     1621 aggccaatgg attttcgcac attgtctctt tgagataaaa tactgaagtc attgataaag
     1681 gcctcgccgt cggttacggg taagattgta gaaagcatat tgatagttgt ggatttgcct
     1741 gcgccgttag gccctaaaaa accaaatatt tctccctttt ttacctcgaa atttatatgg
     1801 tcaaccactg taaaaccatt aaattgtttt gttaaatttt caacgcgtat aatattcata
     1861 aaaatatgta taaagtttta acaaggaata cgattataat aagaacagaa caaacaacaa
     1921 tctgccataa acaggaatgt aatggaattt ctgtagatgt cgcttgaaac gttagtattt
     1981 tttatttatt ttttcagtaa ttttgcgtat tatatctaac tggttagaaa gatgcgatgc
     2041 gaactgttct actttagata attttgtaat cgcaagcatg tcgcatccca taccaaacac
     2101 cagaagttta tcaccttttt taattcccat tgatttacgg gcttctgccg ggatgaccat
     2161 ctgtcctttt tctccaatat ttactgtgcc gtaaaattgc ctttggtttt ctgatttttt
     2221 catattccta caagtgagaa aagtatgatt atgatatgag tatagcaaat cgctaaaaat
     2281 aatacaagtt ttttagtgcc cttaagagga gtcgaacctc tatttcaagc tccgcaagct
     2341 tgtattctat
//
LOCUS       DAOSIB010000142         2330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_39680_length_2330_cov_10.510330, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000142 DAOSIB010000000
VERSION     DAOSIB010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39680_length_2330_cov_10.510330"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..410
                     /locus_tag="PLD44_08670"
     CDS             216..410
                     /locus_tag="PLD44_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOF99435.1"
                     /translation="MVKSNKIKQAREKLGLSQEKLARLADVSNNTIINIEAGKQQNPT
                     IETLKKIAKALNVPIEDLIK"
     gene            415..1431
                     /locus_tag="PLD44_08675"
     CDS             415..1431
                     /locus_tag="PLD44_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOF99436.1"
                     /translation="MKDKTPKQRAPRNRTVILSDSDISKFKKRLIKITKPVSLEEIEN
                     KTIHQDIFEVLDYLPDSFVDLVFVDPPYNLNKTFNLTSFKEMESEKYEQWLDSWVAKI
                     IRILKPHASVYICGDWKSSGAIFNVAKKYFKIQNRITFEREKGRGAKSNWKNCSEDIW
                     FCTLSNKYYFNVDAVKMKRKVIAPYKDENGKPKDWEENTNGKYRITHPSNIWTDITIP
                     FWSMPENTDHPTQKPEKLVAKIILASSKPNDIVFDPFLGSGTTSVVAKKLGRRYVGIE
                     IDEYYCCLAEKRLDMAESEKNIQGYLDGVFWERNTLNEQMNGNGKKNKVGQSEDLFSN
                     KLLL"
     gene            1428..2093
                     /locus_tag="PLD44_08680"
     CDS             1428..2093
                     /locus_tag="PLD44_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99437.1"
                     /translation="MIKKSSIFYTDKYKEIAERIKIFLNTQKDFLSERTLNSPRAVGD
                     AIEDIIADNFQNILGDLVGEYSSSFARRAMADLAFTDKDGFYYVVDVKTHRLDTKFNM
                     PNLTSVERLSRFYEEDVNYFLLLKIDYQIEGTKAVIKHVTFAPIEFFDWNCLTIGALG
                     WGQIQIANANVVNLIPKNSRKKWMLELCDTLLEFYPKEIGKIAERIDHFKKIRKFWEK
                     KKE"
     gene            2095..>2330
                     /locus_tag="PLD44_08685"
     CDS             2095..>2330
                     /locus_tag="PLD44_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99438.1"
                     /translation="MENKQKHLEFIQGTINRMAGNLFFLRGWTITLITGLFALSTKET
                     NQDYILIAYFLILIFWILDGYFLSQERLFRDLYN"
BASE COUNT          818 a          390 c          434 g          688 t
ORIGIN      
        1 ctttttcttt ttgcccgctt gctggaaacc cccaacaatt ttttcggcgg gcttatgatt
       61 ctttcccttg tcccaccctc ccgacgcgga aaagattaag aaactccctt gatgattttg
      121 ctttactttt tcaaaatatt ttggtaatat tatattgtgg atacaagatt taagcatatt
      181 gcaattatac cacaatttaa tattactatt caaatatggt aaagtcaaat aaaataaaac
      241 aagcgagaga aaaactggga ttgtctcaag agaaactggc ccggcttgcc gatgtttcca
      301 ataatacgat tatcaacatc gaagcgggca agcaacaaaa cccaacaatt gaaacgctta
      361 aaaaaattgc taaagcgtta aatgttccaa ttgaggattt aataaaataa aaccatgaaa
      421 gacaagacac caaaacaacg agcaccaaga aacaggactg tcatattgtc tgatagcgat
      481 atttccaaat ttaaaaaacg actgataaaa ataaccaagc cggtttccct tgaagaaatt
      541 gagaacaaaa ccatacatca agatattttt gaagtattag attatctgcc ggatagtttt
      601 gttgatttag tttttgttga tccgccctat aatctcaata aaaccttcaa tcttacttcg
      661 ttcaaggaaa tggaatcaga aaaatatgag caatggcttg attcttgggt cgccaaaatt
      721 attcggatac tcaaaccgca cgcttctgtt tatatttgcg gtgattggaa gtcttcagga
      781 gcaattttta atgtagcaaa aaaatacttt aagatacaaa atcgcataac ctttgaacga
      841 gaaaaagggc gtggagcaaa aagtaattgg aagaattgct ccgaggatat ttggttttgt
      901 acgctttcta ataaatatta tttcaatgtt gacgccgtga agatgaagcg taaagttata
      961 gccccatata aagatgaaaa tggaaagccg aaagattggg aagaaaacac caacgggaaa
     1021 tatagaatta cccacccctc aaatatctgg accgatatta caattccatt ttggtcaatg
     1081 ccggaaaata ccgaccaccc aacacaaaaa cccgaaaaat tggttgccaa gattattttg
     1141 gccagttcaa aaccaaatga tattgttttt gatccgtttc ttggttcagg cacgacttca
     1201 gttgttgcaa aaaaacttgg tcgccgatat gttggtatag aaattgatga atattattgt
     1261 tgtctggcgg aaaagcgttt agatatggca gaaagtgaaa aaaacataca aggatacttg
     1321 gacggcgtat tctgggagcg caacacactt aacgaacaaa tgaatggaaa tggaaagaaa
     1381 aataaagtcg ggcaatcaga agatttattt agtaacaaat tactattatg ataaaaaaga
     1441 gctcaatttt ttatacagat aaatataagg aaatcgcaga aagaattaaa atctttttga
     1501 acacccaaaa agattttctt tcggagcgaa cgctgaacag ccctcgtgct gttggtgacg
     1561 caattgaaga tattattgct gacaattttc aaaatattct tggtgattta gtcggcgagt
     1621 attcttcatc atttgcacgg cgcgcgatgg ccgaccttgc ctttaccgat aaagacggct
     1681 tttattatgt tgttgatgtt aaaacacaca ggttggatac caagtttaat atgccaaatc
     1741 ttacctctgt tgagcgatta tcaagatttt acgaggaaga tgtaaattac tttttgcttc
     1801 taaaaattga ttatcaaatt gaaggaacaa aagctgttat taaacatgtt actttcgcgc
     1861 caatagagtt ttttgactgg aattgcttga cgataggtgc tcttggctgg gggcaaattc
     1921 aaattgctaa cgccaatgtc gtaaatctca tcccgaaaaa tagtcgcaaa aaatggatgt
     1981 tggaattatg cgatacgctt ttagaatttt atccaaaaga aattgggaaa atcgcagagc
     2041 gtattgacca cttcaaaaaa attagaaagt tctgggaaaa gaaaaaagaa taatatggaa
     2101 aataaacaaa aacatttaga gtttattcag ggcactatta accgaatggc gggaaattta
     2161 ttttttctca gaggttggac gattacttta ataactggac tttttgcgtt gtctaccaaa
     2221 gagacaaacc aagattacat cttgattgct tattttctca tcttgatatt ttggattttg
     2281 gacggatatt ttctctcgca agaaagatta tttagagatt tatataacca
//
LOCUS       DAOSIB010000143         2246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_41654_length_2246_cov_11.661798, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000143 DAOSIB010000000
VERSION     DAOSIB010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41654_length_2246_cov_11.661798"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1970)
                     /locus_tag="PLD44_08690"
     CDS             complement(198..1970)
                     /locus_tag="PLD44_08690"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOF99439.1"
                     /translation="MKNIKMTFKELNLKDELLSAIEELGFEQPMPIQERTIPFMQEKS
                     ADLVALAQTGTGKTAAFGLPILNMLDAAQKQTQALILAPTRELCIQISNDLKNYSKYL
                     KGIKVVAVYGGEDIRTQLRQLDTPPQIIVATPGRLIDLTERGKVSLQNIHYLVLDEAD
                     EMLNMGFKEDIETILKQTPENRRTMLFSATMPKEISNIVHQFMKDYEEIIVGQKNSGA
                     SNVEHIYYLTPAKYRYLTLKRIVDLNPDIYGIVFCRTRQETKEVAEKLMQDGYNADAL
                     HGDLSQAQRDNVMQKFRIRNVQLLVATDVAARGLDVSDLTHVINYNLPDDPEVYTHRS
                     GRTGRINKKGISISIIHSKEKHKIKELEKMLKKDFIQQQIPNGLEVCKKQLYHLINRM
                     EHVDVNEEQIQSYLPQIMKQLEYLSKEEIIKRFVSLEFNRFLDYYKDAEDLNEIHFYE
                     DRNNKSKNEKFSKKSGKRIRLKLNVGGREGIDTRRFLGIFNEVVGDKSINVGSIEITN
                     KFTFFDVYADQIDKVMNAFALEPQMDVCEVKGNKQFFEEKPSKFSNHSKKRETYPNIN
                     EGSGRKERTANSEKPWRQSQRTRN"
BASE COUNT          625 a          406 c          418 g          797 t
ORIGIN      
        1 aagtaattaa tgcaactttt tcagggggga ggcaaaaggt aaacaaaaaa ttattttgca
       61 agacaaagga ggaaaaacat tccctcctta tgtcttgatg gaataaaggc tttactttgg
      121 tctctttctt ttaaaaaagt tacgaaaata aaacaaattt tgtaggtgat caggaattgt
      181 ggaaaaaaat caaaaattta attacgtgtc ctttgggatt gtcgccacgg tttttcggaa
      241 tttgcggttc tttctttcct tccggatcct tcatttatat tgggataagt ttcccttttc
      301 ttggaatgat tggagaattt cgaaggcttt tcttcaaaaa attgtttgtt tcctttcact
      361 tcacaaacgt ccatttgagg ctctaaagca aacgcattca ttactttatc tatctgatcg
      421 gcatagacat cgaaaaatgt aaacttattc gtaatttcta ttgacccaac attgatggat
      481 ttgtctccga ctacttcatt gaaaattccc aaaaaacggc gggtatcaat tccttcgcgt
      541 cctcccacat tcaatttcag acgaatacgc tttccgcttt tctttgaaaa tttttcgttt
      601 ttcgatttgt tatttctatc ttcgtaaaaa tgaatttcat tcagatcttc cgcatctttg
      661 taataatcga gaaaacggtt gaattccaat gaaacaaagc gtttgataat ttcctcctta
      721 ctcagatatt ccaattgctt cattatctga ggaaggtaag attgaatttg ttcttcgttt
      781 acatccacgt gttccattcg attaatcaaa tgataaagtt gttttttgca aacttccaaa
      841 ccattgggta tttgctgctg aataaaatct tttttcagca ttttttccag ttccttaatt
      901 ttatgttttt ctttcgaatg gatgatggaa atggaaatac cttttttgtt gattctgcct
      961 gttcttccac ttcggtgtgt gtacacttcg ggatcgtccg gcaaattgta gttgatgaca
     1021 tgcgtgagat cgctcacgtc caatcctctg gcagcaacgt cggtagccac cagcagctga
     1081 acattgcgaa ttctgaattt ttgcatcaca ttgtcgcgtt gtgcttgaga aagatctcca
     1141 tgcaaagcat cggcattata accatcctgc ataagttttt cggcaacttc ttttgtttcc
     1201 tgacgggttc tgcaaaacac aatgccataa atgtcgggat tgaggtctac aatccgtttt
     1261 aaagtcaaat aacgatattt ggccggagtg aggtagtaaa tatgttctac attggaagct
     1321 ccggaatttt tttgtcccac tattatttct tcataatctt tcatgaattg gtgaacgata
     1381 ttcgaaattt ctttgggcat ggtggctgaa aaaagcatgg tacgtctgtt ttcaggcgtt
     1441 tgtttcagaa tggtttcaat atcttcttta aaacccatgt tcagcatttc atcagcttca
     1501 tcgagcacca aataatggat gttttgaaga gaaacttttc cgcgttcggt gaggtcgatt
     1561 aatcggcccg gagtagctac aataatttga ggtggtgtat caagctgtct taattgggta
     1621 cgtatatctt ctccaccata aacagctaca actttgattc ctttcaaata tttggagtaa
     1681 tttttcagat cgttgctgat ttgaatacat aattctctgg tcggagccaa aatcagtgcc
     1741 tgagtttgtt tttgagcagc atccagcatg ttgagaatag gaaggccaaa agcagctgtt
     1801 tttcctgtcc ctgtctgcgc caaagccacc aaatcggcag atttttcctg cataaaaggg
     1861 atggtacgtt cctgaatggg catgggttgc tcgaatccga gctcttcgat agccgaaaga
     1921 agctcgtctt ttaaattcaa ttctttaaat gtcattttaa tgtttttaat acacgttttt
     1981 gagcattcat ccgatgtaaa aattttattg aaagataaaa cgtgtacttt gagatgcaga
     2041 tttcaaaaat caaaaatata atgtcttgaa tttcggatat ttactcgaaa cgtatagttg
     2101 ttttttaatt cgggtgcaaa gatagttatt tattttcata tttgtatggt attgtcgatt
     2161 ttgtttttgt tctatagttt gtcatgaaac gcacatgtat tgcatacaca aacacgaatg
     2221 aatataatat tcagacatgt gggttc
//
LOCUS       DAOSIB010000144         2162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_43868_length_2162_cov_9.704794, whole genome shotgun sequence.
ACCESSION   DAOSIB010000144 DAOSIB010000000
VERSION     DAOSIB010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43868_length_2162_cov_9.704794"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /gene="nadA"
                     /locus_tag="PLD44_08695"
     CDS             <1..842
                     /gene="nadA"
                     /locus_tag="PLD44_08695"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOF99440.1"
                     /translation="IQDIADHVGDSLALAQWAAQTDADIIVLCGVHFMGETAKILNPQ
                     KKVLIPDMNAGCSLADSCPADAFEEFVKNHPDYTVISYVNTSAAVKALTDVVVTSTNA
                     KKIVESFPKEEKIIFAPDKNLGNYINSITGRQMLLWNGACHVHEQFSVQKLVELKKKY
                     PEALVLAHPECTKPLLMLADFIGSTQALLNFATSSDKKFFLVATESGILHEMQKKNPD
                     KQFIPVPPNDSTCACNECNFMRLNTLEKVYQCLLNESPEILVEEEIRQKAVKPILRML
                     ELS"
     gene            871..1683
                     /locus_tag="PLD44_08700"
     CDS             871..1683
                     /locus_tag="PLD44_08700"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOF99441.1"
                     /translation="MILPIYFLPLAGLLIGILISLMGGGGGIFYIAILTGLFSIPMNQ
                     AVSVSLATIIPTTFAASISHFKAGNVNINVGLILSAGGIIGVIAGSFLLGLLSLNVLK
                     IVFGLFMIFMAVQMIFKNFRSKKSFNKGNIILVEEDKTVNIAKKKKIFSFKNFKGLSF
                     GFFGGIMSGLLGVSGTPPILAGLYNMKLSSVQVVGTSVMVLFFIALSGVITHTAFGHL
                     NWYLVGLLAAGTITGAILGPILGKKIDEKKLEKFYTPFFIVFIFLMGISMFF"
     gene            1820..>2162
                     /locus_tag="PLD44_08705"
     CDS             1820..>2162
                     /locus_tag="PLD44_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOF99442.1"
                     /translation="MTQVTDKVNTSGSKNTQSNVVVDWFIRLIKGIIVGIGFILPGLS
                     GGVLAVILGIYDRIIKFLSDLRKDFVQNVFYFIPVVIGGAIGIVLFSILVEKAFGNYA
                     AQFICLFVGFVG"
BASE COUNT          637 a          318 c          437 g          770 t
ORIGIN      
        1 atattcagga tattgccgac catgttggag acagtttggc attggcacag tgggcagcgc
       61 aaacggatgc cgatattatt gttctttgtg gggttcattt tatgggagaa acagcaaaaa
      121 ttcttaatcc gcaaaaaaaa gtgctgatac ctgatatgaa tgccggttgt tcgcttgctg
      181 acagttgtcc ggcagatgcc tttgaagaat ttgtaaaaaa tcatcccgat tatacggtta
      241 tttcttatgt caatacatcg gctgctgtta aagctttaac tgatgttgta gtaacttcaa
      301 ccaacgccaa aaaaattgtg gaatcttttc caaaagaaga gaaaattatc tttgcaccag
      361 ataaaaattt gggaaattac atcaacagca taaccggccg acaaatgctt ttgtggaacg
      421 gagcttgtca tgtacacgag cagttttcgg tacaaaaact tgtggaattg aaaaagaaat
      481 atcccgaagc attggttttg gctcatcccg aatgtacgaa accactttta atgctggccg
      541 attttattgg cagcacgcag gctttattga attttgctac atcatccgat aaaaaatttt
      601 ttttggttgc aacagaatcg ggtattttgc acgaaatgca aaagaaaaat cccgacaagc
      661 aatttattcc agttccacca aacgacagca cgtgtgcgtg caacgaatgc aatttcatgc
      721 gtttaaatac gcttgaaaag gtatatcaat gtcttttaaa tgaaagtcca gagattttgg
      781 ttgaggagga aatccgacaa aaagctgtaa aacctatttt gcgaatgttg gaattaagtt
      841 gaaaaaaaat tgtataacta atttttaata atgattttac ctatttattt tttgccatta
      901 gccggtttat tgattggaat actaatttct ttgatgggag gcggaggagg aattttttat
      961 attgccattt tgactggatt attttccatt cctatgaatc aggcagtttc tgtttcactt
     1021 gccaccatca taccaactac ttttgcagcc tctataagcc attttaaggc tggaaatgta
     1081 aatataaacg taggattgat tctttcagcc ggtggaatta ttggagttat agcgggctct
     1141 tttttattgg gacttttatc cttgaatgtt ttaaaaattg tatttggttt gttcatgata
     1201 tttatggctg tgcaaatgat ttttaaaaat tttcgttcaa aaaaatcttt caataaggga
     1261 aatattattt tagtagaaga agataaaact gtaaacatag caaaaaagaa aaaaattttt
     1321 agtttcaaaa attttaaggg tttaagtttt ggtttttttg gaggaattat gtccggtttg
     1381 ctgggagtaa gtggaactcc acccatttta gccggacttt acaatatgaa gttgagttcc
     1441 gtgcaggtgg tgggaacgtc agttatggta ctttttttta ttgctctttc aggagtcata
     1501 acacataccg cctttggtca tctcaattgg tatttggtag gattattggc tgcgggaaca
     1561 attaccggag ctattttggg cccaattttg ggaaagaaaa ttgacgaaaa aaaattggaa
     1621 aagttttaca cgccattttt tattgttttt atttttttga tgggtatttc catgtttttt
     1681 tgataaagca tttgttaaag tatattttat atctttacat ctaacatttc atttctgttt
     1741 ttttctatcc aaaattttct tttctccaat tgaacgaaaa aagtgtaact ttgcaagcga
     1801 aatttttaaa aattttttta tgactcaagt aactgacaaa gtgaatactt ccggctcgaa
     1861 aaatactcaa tcaaatgttg ttgttgactg gtttattaga ttgataaagg gtataattgt
     1921 gggtattggt tttattttac ccggtttgtc gggcggagtg cttgcagtaa ttttgggaat
     1981 ttatgacaga attatcaaat ttttgtccga tttgcgaaag gatttcgttc agaatgtttt
     2041 ttattttatt ccggtagtaa ttggcggggc aatcggaatc gtgctttttt ctattttggt
     2101 ggagaaggct tttggtaatt atgccgctca atttatttgc ctttttgtgg gatttgtagg
     2161 cg
//
LOCUS       DAOSIB010000145         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_48675_length_2004_cov_11.970754, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000145 DAOSIB010000000
VERSION     DAOSIB010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48675_length_2004_cov_11.970754"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1836)
                     /locus_tag="PLD44_08710"
     CDS             complement(130..1836)
                     /locus_tag="PLD44_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOF99443.1"
                     /translation="MKKHLFFAISVVMAVLLSSCSKNMGALDPSNFKCTPNPLELKGG
                     KVDAAINGTFPVKYFNKNATVTVTPVLRFAGKEVKSTPVTFQGEKVVGNNKTISYKAG
                     GSYTINASFNYVPEMAKSELYLDFNVTVKKKTYQIPSVKVADGVIATAELVAVNASEM
                     NAAVIPDKFQRIIKETQNADIKFLIERWDLRPNELKKDEVVSLVEKIKEVKDAQNKEV
                     SSFEILGYASPDGPIDLNTNLAEKRQDVTSDYINKQMKKLKTSVKIDSKFTPEDWAGF
                     QALMEKSNIQDKDVILRVLSMYTDPEERERQIKNLSVAYTKIAEEILPELRRSQLKLT
                     VDVIGKSDAEIMQLVSNDPSKLNLEELLYAATLTNDANAKASIYKKVTELYPNCVRGF
                     NNLGAVYFQQGKLDEASAMLTKAMQLNPNSPDVNYNCALIALAKKDLSKAEELLGKAA
                     GTSGDLSKALGTLYIMKGDYAKAKTSFKTNDVNNNVALLQILSGDYNSARKTLSAIQS
                     PNATTAYLSAIIGARTNDRETVYSNLKLAISKDKSMAAKALNDIEFAKFATDQTFLSI
                     VQ"
BASE COUNT          577 a          371 c          370 g          686 t
ORIGIN      
        1 taggggtaaa tgtttaaaat tcatttactc gctttttgag ataaaccgta agtaaaacca
       61 tgtctatatt cgtaaaaaaa gggaatgcct tttgtgaagc atcccctttt taataaacta
      121 taaattcgat tattgaacaa tcgaaaggaa agtttgatcg gttgcaaact tggcaaattc
      181 gatatcgttc aaagcttttg cagccattga tttatctttg ctgatagcca gtttaagatt
      241 cgaataaact gtttctctgt cattggtacg ggctccaata attgcgctca aataagcggt
      301 ggtagcattc ggagactgaa tagccgacag ggttttacga gccgaattgt aatcgccact
      361 caaaatttga agcaaagcca cattattgtt cacatcattg gttttgaaag aagttttagc
      421 ttttgcataa tcacctttca taatataaag cgttcccaaa gctttgctca agtcgccgga
      481 agtgcctgcg gcttttccca ataattcttc agctttggaa aggtcttttt tagctagtgc
      541 aatgagtgcg caattataat ttacgtcggg agaatttgga ttaagctgca tggcttttgt
      601 caacatggca gatgcctcat ccagttttcc ttgttgaaag taaacggctc ccaaattgtt
      661 gaaaccacga acgcaatttg gataaagttc ggtaactttt ttgtagatgg aagcttttgc
      721 attggcatca ttggtcagcg tagctgcata aagcaattct tccaaattca gcttggaagg
      781 atcatttgaa accaattgca taatttctgc atccgatttg ccaataacgt caaccgtgag
      841 tttcaactgt gaacgacgaa gttccggtaa aatttcttcg gcaatttttg tataggcaac
      901 cgaaaggttt ttaatctgac gttcacgttc ttccggatcg gtgtacatgg acaaaacgcg
      961 aagaatcaca tctttatcct gaatattgga tttttccatc aaagcttgga accctgccca
     1021 gtcttcagga gtaaatttgg agtctatttt tacagaagtt ttgagttttt tcatttgttt
     1081 gttaatgtaa tcggaagtta catcctgacg tttttctgcc aaattggtat tcaaatcaat
     1141 cgggccatca ggagaagcat aacccagaat ttcaaatgaa gaaacttctt tgttctgtgc
     1201 atctttcact tcttttattt tctctaccaa agaaactact tcatcctttt ttaattcatt
     1261 gggacgaaga tcccatcttt caatcaagaa tttaatgtcg gcattctgtg tttccttaat
     1321 gatacgttga aacttgtcgg gaattacagc tgcattcatt tctgaagcat tcactgcaac
     1381 caattctgca gtggcaatca ctccatcggc aactttaaca cttggaattt gatacgtttt
     1441 cttttttacg gtaacattga agtcgagata gagttccgat ttggccattt caggcacata
     1501 gttgaaggaa gcgttgatag tgtaggaacc gccggctttg taggaaatgg ttttgttgtt
     1561 acctacaact ttttctccct gaaaagttac cggtgtactt ttaacttctt tccctgcaaa
     1621 acgtaaaacg ggagttacgg ttacagtagc attcttattg aaatacttta ccggaaaagt
     1681 accattgata gcggcatcca cttttccacc ttttaattcc agcggattgg gagtgcactt
     1741 aaaattgctg ggatcaagtg ctcccatgtt tttactgcaa gagcttaata acactgccat
     1801 taccacagaa atggcgaaaa ataaatgttt cttcatactt taattttttt tattgttaac
     1861 cttaaaattt cgatataaat tttattcagc agccaaagtt attatttttt gtccaattat
     1921 tctttatttt gcaaaaaatc ttttcagaat tttttaaaag ataacatctc agggttttga
     1981 aaagtttttg gttttagtaa aaaa
//
LOCUS       DAOSIB010000146         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_51118_length_1935_cov_8.626064, whole genome shotgun sequence.
ACCESSION   DAOSIB010000146 DAOSIB010000000
VERSION     DAOSIB010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51118_length_1935_cov_8.626064"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(350..496)
                     /locus_tag="PLD44_08715"
     CDS             complement(350..496)
                     /locus_tag="PLD44_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99444.1"
                     /translation="MSTYEQLEALAPYILPKEILDDFDIVGIEPKLLISQFKGKVKLR
                     RAVP"
     gene            complement(673..1713)
                     /locus_tag="PLD44_08720"
     CDS             complement(673..1713)
                     /locus_tag="PLD44_08720"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOF99445.1"
                     /translation="MKSEESSQITVHGKTVKLFTLINKNGMSCSITNFGAKVVRLFAA
                     DKEGKFEDVVLGFDTLTECMEREPYFGAVCGRFANRIKDGKFVLDGVEYHLPINNGTN
                     SLHGGIHGFNEKIWEVKSVDNQEIIMEYLSVDGEEGYPGNLTVKVTYFLSDNNELKIH
                     YEAVTDKATVINLTNHSYFNLKGAGNGTIRNHILQLNADFYTVLDDSFAPTGEIRPVE
                     NSAFDFRQPTVIEQRIDDEAFAPGRGLDNNWAIRKHHTGEMVVAGFLYEPESGRKMEV
                     MTTQPGVQVYSGNWIDKQTGKNGKVYESQHAICLETQGFPNSANVPFFPTPVLRPGKK
                     YDEWCIYRFSAE"
BASE COUNT          571 a          374 c          336 g          654 t
ORIGIN      
        1 gagtagcaat agaaaaaaac gtgtgtgaac tttggtatat ccttacaaac aaagaaataa
       61 caccatgtgg tggaatgatt ttttttgtaa caaatgtttg aaaaaccacg tagaatcgtt
      121 attgttcgtc aaaaattaaa ggaaagcact ttcgtcttta attttgtgat gatagcctat
      181 aatcttatgt cgctgtttag aatgttcatt ttgcagagaa aaacgcagaa aactttatct
      241 acacacttag atataaaact tttgccattg gtgcatattt tgaaaaaatc aacaacaaac
      301 tgcttctgta aatcgcttta aataaaaaag gcgaacatga ttttccggcc tatggaacag
      361 ctcttctaag tttgactttc cctttaaatt ggctaatgag taatttgggt tcaattccaa
      421 caatatcaaa atcgtctaat atctctttcg gaagaatgta aggggcaagt gcctcaagct
      481 gttcgtaagt ggacatataa aaagtatttt ttcacaaaga tacttctctt tttctacaaa
      541 ctcatatttt tccatgtgag ccataaacga cagaaattag aaaaaattct ctctaatttc
      601 tgtcgttaaa atgtttccct atgatttgaa aagcatcgac ttacaggaaa aatataattt
      661 attttccctt ccttattccg ccgaaaatct gtaaatacac cactcatcgt atttttttcc
      721 gggacgtaat acgggagtgg ggaagaaggg tacattggct gaattgggaa atccttgcgt
      781 ttcgagacaa atggcatgtt gactttcata gactttcccg tttttccccg tttgtttatc
      841 tatccagttg cccgaataaa cctgcactcc cggttgtgtt gtcatgactt ccatttttct
      901 gcccgattca ggttcgtaca gaaatccggc tacaaccatt tctcctgtat gatgtttccg
      961 aatagcccaa ttgttgtcta atcctcttcc cggagcaaaa gcctcatcat caatccgctg
     1021 ttcaattaca gtgggttgac gaaaatcgaa agccgagttt tctacgggac gaatttctcc
     1081 tgtcggcgca aaagaatcgt ccagtacggt gtaaaaatcg gcattcaact gtaatatgtg
     1141 gttgcgaata gttccgttgc cggctccttt gagattgaaa taggaatgat tggtcagatt
     1201 gatgaccgtt gctttatcgg ttacggcttc gtaatgaatt tttaactcgt tgttgtccga
     1261 aagaaaataa gttaccttta ccgtgagatt gcctggataa ccttcttcgc catctaccga
     1321 cagatattcc ataataattt cttgattgtc aaccgattta acttcccaga ttttttcatt
     1381 gaagccatga atgccgccat gaagagaatt tgttccgttg ttgatgggca aatgatattc
     1441 aacgccatca agaacgaatt tgccatcttt aattcggttg gcaaagcgtc cacatacagc
     1501 tccgaagtat ggttcacgtt ccatacattc ggttaatgtg tcaaaaccca acaccacatc
     1561 ttcaaatttt ccttctttat cggcagcaaa tagcctcacc acttttgctc caaagttggt
     1621 gatgctgcat gacatcccgt ttttattgat taaagtaaat agttttactg ttttaccgtg
     1681 aactgttatt tgtgaagatt cctcgctttt catgtctaac tcctgtttaa attgctttta
     1741 ttgtaatatc ataaatcatt tcaataaata ttaggttttt gttttgattg tatctaatta
     1801 tttcggataa acttcaaaaa tattgaagtt tatccgaaat aaaattttca ttgaaaatga
     1861 attagctcaa tcggcaagca ccatcaccta tttcggcaat ataaaattcg ggtttgattc
     1921 cgatttttgc ttcat
//
LOCUS       DAOSIB010000147         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_55770_length_1817_cov_16.874574, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000147 DAOSIB010000000
VERSION     DAOSIB010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55770_length_1817_cov_16.874574"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..866
                     /locus_tag="PLD44_08725"
     CDS             351..866
                     /locus_tag="PLD44_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOF99446.1"
                     /translation="MSWEIIVPHEIPEKSVICFAPHTSNCDFVIGKLFSWSVGMQFSF
                     LVKKSWFVFPLGYFFKAVGGIPIDRSKSNATVEQMIAEFEKHDRFHLVIAPEGTRSYV
                     PAWKKGFYYIASGANVPIELAFIDYKKKQIGIGKLLYPSGNFSSDFSEIQQFYQNISP
                     RYPEKYNPTIF"
     gene            905..1678
                     /locus_tag="PLD44_08730"
     CDS             905..1678
                     /locus_tag="PLD44_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOF99447.1"
                     /translation="MKITLIQDVIAWSDKQANFNRINRHLETLQGETDLVVLPEMFST
                     GFCTNRLDLAEEGKGETVQTLSNWAKNFGIALTGSFIACENNRYFNRSFFVFPDGNIQ
                     TADKRHLFRHGGEHQYFSAGNRHLLVHYKKWNICVLVCYDIRFPVWSRNVDNRYDLLI
                     YTANFPSKRIRDWDILLQARAIENQAYVCGLNRVGTDGQGIEYNGHSTLVDFKGRFLL
                     TFPENIFDIKTAEISLDSLQNYRQHFPVWMDADRFQIIE"
BASE COUNT          574 a          297 c          336 g          610 t
ORIGIN      
        1 aaaatctaat tgtacatcac ttttttcatt tcatcagaat ataatttatt ttttaagggt
       61 ttgatgaaac ccacatgtct gaatattata ttcattcgtg tttgtgtatg caatacatgt
      121 gggtttcata aaaattttat gctttttgat ctccatctct attaaaaata attaactttg
      181 aaaaactttt tcggaagaaa cttaaacata gaaaaataat ttactgtttt gggaaataaa
      241 aacaacattg aactgaatct ggtcaactga aatattgttt ttatttgctt tttaaaattt
      301 tcctcgagac taaaatgatg aaaaagttaa tttatttgtt ttttaaaagc gtgagttggg
      361 aaataattgt tccccatgaa attcctgaaa aaagcgtaat ctgttttgct ccacacacca
      421 gcaattgtga tttcgttatc ggcaaacttt tctcgtggtc agttgggatg caattttcgt
      481 ttttggtgaa gaaatcatgg tttgtgtttc cgttgggtta tttttttaag gctgtcggag
      541 gtattccgat cgatcgctcc aaaagcaatg ccacagttga gcaaatgata gcagagtttg
      601 aaaaacatga tcgttttcat ttggttatag cgcctgaagg aaccagaagt tacgtgccag
      661 cttggaaaaa aggtttttat tatattgctt ccggtgccaa tgtgcctatt gaattggcct
      721 tcattgatta caaaaaaaaa caaatcggga tagggaaatt gctttatcca tcgggaaatt
      781 tttcatctga cttttctgaa attcagcagt tttatcaaaa tatttctccc cgatatcccg
      841 aaaagtataa tccgactatt ttttagaaaa ttgaatcgga ttgattttta aaatttataa
      901 aaatatgaaa atcacgctta ttcaagatgt tatagcttgg tcagacaaac aagccaattt
      961 taacagaata aaccgacatt tggaaacctt gcaaggggaa accgatttgg tggtattgcc
     1021 cgaaatgttt tcgaccggat tttgtaccaa tcgcctcgat ttggctgaag aagggaaggg
     1081 ggaaacggtt caaacacttt caaattgggc taagaatttt ggtattgccc taacaggaag
     1141 ttttatagcc tgcgaaaata atcgttattt caaccggagt ttttttgttt ttcccgatgg
     1201 caacattcaa actgccgata aacggcattt gtttcgacat ggtggagaac accaatattt
     1261 ttctgccggc aaccgacacc tgcttgttca ttataaaaaa tggaatatct gtgtgttggt
     1321 ttgctacgat atacgttttc ctgtttggtc gagaaatgtg gacaatcgtt acgacctgct
     1381 gatttatact gccaattttc cttccaagcg aattcgcgat tgggatattc tgttgcaagc
     1441 tagagctatt gaaaatcaag cgtatgtgtg tggcttaaac cgagttggaa ccgatggaca
     1501 gggtatagaa tataacggac attctacatt ggtcgatttc aaagggcgtt ttttgctcac
     1561 gtttccagaa aatatttttg atataaaaac tgcagaaata tcactcgatt ctctacaaaa
     1621 ttaccgtcaa cattttccgg tgtggatgga tgccgaccga tttcaaataa ttgaataata
     1681 actttttatg aaacccacat gtattgcata cacaaacacg aatgaatata atattcagac
     1741 atgtgggttc catcagaccc ttaaaaataa attatattct gatgaaaaac aaaatattga
     1801 gtccaattca aaaagtt
//
LOCUS       DAOSIB010000148         1753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_58617_length_1753_cov_12.210836, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000148 DAOSIB010000000
VERSION     DAOSIB010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58617_length_1753_cov_12.210836"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..690)
                     /locus_tag="PLD44_08735"
     CDS             complement(148..690)
                     /locus_tag="PLD44_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF99448.1"
                     /translation="MLENENIRLRAVEPEDLELLYRWENNPELWMVGNTRNPYSRYML
                     KRYILDSEKDIYENRQLRLMITIKSSNETIGTVDLFDFDIHNSRIALGLFIEQKFQRN
                     GFAREALHLVEEYVFEFLKINQLYVQIAEENIPSMRMFEEENFEKNGMLKNWIKTTND
                     FQNIIVFQQFKNVYNKTKMK"
     gene            complement(696..1718)
                     /gene="recA"
                     /locus_tag="PLD44_08740"
     CDS             complement(696..1718)
                     /gene="recA"
                     /locus_tag="PLD44_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOF99449.1"
                     /translation="MAEKTTEEQKKPSSEKIKALQLAMEKIEKDYGKGTIMRMGDNQI
                     VDIDVIPTGSIGLNIALGVGGYPRGRVIEIYGPESSGKTTLAIHAIAEAQKAGGIAAI
                     IDAEHAFDRAYAEKLGVNTDELLISQPDSGEQALEIADQLIRSSAVDIVVIDSVAALT
                     PKAELEGDMGDSKMGLQARLMSQALRKLTANISKTNTTCIFINQLREKIGVMFGNPET
                     TTGGNALKFYASVRLDIRKIGQLKDGEEVIGHQTRVRVVKNKVAPPFRKAEFDIMFGE
                     GISKSGEILDLGVEYGIIKKSGSWFSYGDTKLAQGRDAAKTVIKDNPELAAELEAKIV
                     EAIKEK"
BASE COUNT          449 a          366 c          332 g          606 t
ORIGIN      
        1 attccgcaag gagaggtaaa attgctggaa gtatatattc agtaaggatt atttcaaact
       61 taatattaaa aagggaatgg ctgcaaacga gagttgaagt gccattccct ttttcctgtt
      121 ttattctgtc aaaacaaatc tatcacatca cttcattttt gttttattgt aaacattttt
      181 gaattgttgg aaaacgataa tattttgaaa atcgttcgtg gtttttatcc aatttttcaa
      241 catgccgttt ttttcaaaat tttcttcttc aaacatgcgc atgcttggga tattttcttc
      301 agcaatttgt acgtaaagtt ggttaatttt caaaaattca aatacatatt cttcaacgag
      361 atgaagtgct tctctggcaa aaccgtttct ttgaaatttt tgctcgataa aaagtcccaa
      421 tgctatgcgg ctattgtgaa tgtcgaagtc aaacaagtca accgttccaa tggtttcatt
      481 gctgcttttg atagttatca tcaaccgcaa ttgcctgttt tcgtatatgt ctttttccga
      541 atccagtatg taccgtttca gcatataacg tgaataggga tttcgggtat tgcccaccat
      601 ccacaattcg ggattgtttt cccatcgata cagaagttcc aaatcttcgg gttcgacagc
      661 acgtagccgt atgttttcgt tttccagcaa caattttatt tttctttgat agcttcaaca
      721 atttttgctt cgagttcggc agccaattct ggattgtcct taattacggt ttttgcggca
      781 tctcttccct gagcaagttt ggtatcgcca tagctgaacc acgaaccact ttttttgata
      841 attccgtatt ctacgcccaa atcgaggatt tcaccgcttt tggaaattcc ttcgccaaac
      901 ataatatcaa attctgcttt acggaaagga ggtgcaactt tatttttcac tactctcaca
      961 cgcgtttgat gtcctataac ttcttctcca tctttcagtt gcccgatttt tcgaatatcc
     1021 aaacgaacgg aagcatagaa tttcagggcg tttcctccgg tagtagtttc aggattgcca
     1081 aacataacac caattttttc ccgaagttga ttgatgaaaa tacaggttgt gttggttttg
     1141 ctaatgttgg cagtaagttt gcgaagtgcc tgcgacatta atcgggcttg aagtcccatt
     1201 tttgaatcgc ccatgtcgcc ttccagttcg gcttttggag ttaaggcggc aacggaatcg
     1261 ataacaacaa tatcaacggc tgaggaacga atcagttgat cggcaatttc caacgcctgt
     1321 tcgccactgt cgggctgaga aatcagcaat tcgtcggtat ttacacccaa cttttcggca
     1381 taagcacgat cgaaagcatg ttctgcatca ataatggcag caatgccgcc ggctttctga
     1441 gcttcagcaa tggcatgaat ggccaatgtg gtttttccgg acgattcggg tccgtaaatt
     1501 tcaatcactc tgcctctggg atagccgcca acgcctaaag caatgtttaa acctatggag
     1561 ccggttggaa tcacatcgat gtcaactatt tggttgtcgc ccatacgcat aatggtccct
     1621 tttccgtaat ctttttctat tttttccatt gccagctgca aggcttttat tttttcggaa
     1681 gacggtttct tttgttcttc ggttgttttt tcggccataa ttaagttttt aataattgat
     1741 tatttttctg aac
//
LOCUS       DAOSIB010000149         1712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_60717_length_1712_cov_11.022330, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000149 DAOSIB010000000
VERSION     DAOSIB010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60717_length_1712_cov_11.022330"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PLD44_08745"
     CDS             complement(<1..605)
                     /locus_tag="PLD44_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99450.1"
                     /translation="MKNTIRITAILVVFLTISPPFFSQNSIKSVEFWTDGNGENRNRQ
                     PISSSNTFIWQDEINLAALVDGLHTFNMRIQDSKGVWSQVETLFFLKQTNAAGQGVLK
                     KIAALEYWMNGNSANRTQRVLNGQTSFTWEELIDVAGIPDGANTFNARFKDEYGVWSP
                     IETRFFLKQTNAAGQGVLKKIVALEYWMNGNSANRTQRTLNG"
     gene            complement(696..>1712)
                     /locus_tag="PLD44_08750"
     CDS             complement(696..>1712)
                     /locus_tag="PLD44_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99451.1"
                     /translation="ITIKTQLMKKLTILLICVWAMMLAFTFSSSAQTVFALQNGSRTQ
                     FYTTLSTAISNIQDGDTLYLPGKPLDGDYTFAKKVTIIGAGYDPDSAAATGITKITGR
                     VYFAKNSKGSSITGVRTEGRVYVRDSLITITRSNLESLEIQNGSNPIGFVYVGDCIIR
                     NNIEGANDVVTNVLIERCILNGNLGGGNKTNNLVIKNCVLTYNGNYFLYSISNIIYQN
                     SIILNTNNYMFTAGSSSLTFTNNLFVRPAFSNNTYVMTGNIFGVPESDIFVDSANGNY
                     HIKPTCTQALTLATDGKEVGIYGTDNPFLVPTFAPRFISINNAESTVNGKLSVKMTVE
                     ARNR"
BASE COUNT          504 a          341 c          330 g          537 t
ORIGIN      
        1 ccgttcaagg ttcgctgtgt tctgttggca gaatttccat tcatccaata ttcaagagcg
       61 acaatttttt tcaaaacgcc ctgtccggca gcattggttt gcttgagaaa aaatcgggtt
      121 tcaatgggcg accaaacacc atattcgtcc ttgaaacggg cattgaacgt gttggcacca
      181 tcgggaatac cggcaacatc aatcaactct tcccaagtga aagaagtttg tccgttcaaa
      241 actcgctgtg ttctgttggc agaatttcca ttcatccaat attcaagagc ggcaattttt
      301 ttcaatacgc cctgtccggc agcattggtt tgcttgagga aaaatagggt ttcaacttgc
      361 gaccaaactc ctttgctgtc ctgaatgcgc atgttgaaag tatgcaatcc atccacaagt
      421 gcagccaaat tgatttcatc ttgccaaata aaagtatttg aagaggaaat tggctgacga
      481 ttgcgatttt caccgttgcc atccgtccaa aattcgaccg atttgattga attttgtgaa
      541 aaaaaaggag gtgaaatggt aagaaacacg acaagtattg ctgtaattct tattgtattt
      601 ttcattttgt gatttgtttt gaaaaatcat tttaatagat tgattttcaa acgaataata
      661 atgtcggata atttctgaac tttgcattgc tgtccttatc tgtttcgtgc ctctacggtc
      721 atttttaccg atagctttcc attgacggta ctttcggcat tgttaatgga aataaatcgt
      781 ggcgcaaacg ttggaaccag gaatggattg tccgtgccat aaattcccac ttcctttcca
      841 tcggtagcca gtgtcagtgc ctgtgtgcag gtcggtttga tatgatagtt cccatttgct
      901 gaatctacaa atatatcact ttcaggcacc ccaaatatat ttcccgtcat aacataagta
      961 ttattagaaa aggcaggacg tacaaataaa ttgttagtaa acgtaagaga agaactacca
     1021 gctgtaaaca tatagttatt ggtgtttaaa attatcgaat tttgatatat aatgttgctg
     1081 atactatata aaaaataatt accattatac gttaaaacac aattttttat cactaaatta
     1141 ttggttttgt ttccacctcc caaatttccg ttgagaatgc agcgttctat caatacattg
     1201 gtaaccacat catttgctcc ttcaatgtta tttcgaataa tacaatcgcc cacatataca
     1261 aatccaatcg gattactgcc gttttgaatt tcaagcgatt ccagattact tcgcgtaatg
     1321 gtaatcaagg aatcacgcac gtaaacgcgc ccttccgtac gaacaccggt aatggaagag
     1381 cctttgctgt ttttcgcaaa ataaactctg ccggtaattt ttgttattcc ggtggccgca
     1441 gcactgtcgg ggtcatagcc ggcaccgata atggtaacct tttttgcaaa agtataatct
     1501 ccatctaacg gtttccccgg caaatagagt gtatctccat cctgaatgtt gctgatggca
     1561 gtggacaaag tagtataaaa ctgggttctg cttccatttt gaagcgcaaa aacggtttgg
     1621 gcactggaag agaatgtgaa agcgagcatc atagcccata cacaaataag gagaatagtt
     1681 aactttttca taagctgagt tttaatagta at
//
LOCUS       DAOSIB010000150         1697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_61506_length_1697_cov_8.959196, whole genome shotgun sequence.
ACCESSION   DAOSIB010000150 DAOSIB010000000
VERSION     DAOSIB010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61506_length_1697_cov_8.959196"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PLD44_08755"
     CDS             complement(<1..342)
                     /locus_tag="PLD44_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99452.1"
                     /translation="MDQNRISQLQSVFDEISHFITSDDDREQAGVWFARELMVALGYA
                     RWENFQVAIGRAVDSCKIQNINVDDHFREVTKMVEIGKTSKNLNVVWLPKKNKLKRQV
                     PNCPKATTNNKS"
     gene            complement(373..1515)
                     /locus_tag="PLD44_08760"
     CDS             complement(373..1515)
                     /locus_tag="PLD44_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOF99453.1"
                     /translation="MIELEKYQAGHFEKNLGYKYFVPSTINDEWTWKTPALNLLLEKA
                     AIKLGELNSYARLVPNIDLFIQLHVTKEAVVSSHIEGTKTNMDEALLPVEEIHPERRD
                     DWQEVNNYIKALNNAIEELKSLPISSRLLKETHKILLQSVRGEHKLPGEFRTSQNWIG
                     GTSLHDAVFIPPSHHLVNELMSDLEKFLNNDEINVPLLIRIAIAHYQFETIHPFLDGN
                     GRIGRLMITLFLVSEGILDKPLLYPSLYFEKNKSLYYDNLSNVRTKNDMLQWIKYFLV
                     GIEQSAAKAVETLSNILKLKESIEKEINSNFGRRSNSAHTLVHFLFKDPVISIDKAAK
                     ECNLSFKAANDLVALMQKFNYLKELTGQSRNRIFIFEPYLKIFDND"
BASE COUNT          487 a          323 c          285 g          602 t
ORIGIN      
        1 ggatttattg tttgttgtcg cttttgggca atttgggact tgccttttca atttgttttt
       61 cttcggcagc cacacgacgt tcaagttttt tgatgttttc ccaatttcaa ccattttcgt
      121 gacctcacga aaatgatcat cgacattgat attctgtatt ttacatgagt caacagctct
      181 gccgattgcg acctgaaaat tctcccaacg ggcatatccc aatgcgacca tcaattcccg
      241 ggcaaaccaa actcccgctt gttctctatc gtcatcactt gtaataaagt gactaatttc
      301 atcgaaaact gattgtaatt gtgaaatgcg attttgatcc atatttatag aatgatttga
      361 tatatccggt cgtcagtcat tgtcaaaaat ctttaagtag ggctcaaaaa taaaaatacg
      421 gttgcggctt tgcccggtta attccttaag gtaattaaat ttttgcataa gtgctactaa
      481 atcattagct gctttgaaac tcagattaca ctctttcgct gccttatcaa tagagataac
      541 cggatcctta aatagaaaat gaacaagcgt atgagcagaa ttgcttcgcc gaccgaaatt
      601 gctatttatc tctttttcaa tgctttcttt aagttttaat atattagaaa gtgtttctac
      661 cgcttttgca gcactttgtt caatgcctac taaaaaatat tttatccatt gcagcatatc
      721 gtttttggtt cttacattac ttaaattatc gtaatacagg cttttatttt tttcgaaata
      781 caatgatgga tagagtaaag gtttatctaa aataccttct gaaacaagaa ataaagttat
      841 cataagacgt ccgatacggc cattcccgtc taaaaacgga tgaatagttt cgaattgata
      901 atgtgcaatg gcaatacgta ttagcagagg aacgttaatt tcatcattat taagaaactt
      961 ttccaaatcg ctcataagtt cattcaccaa gtggtgagat ggcggaataa atactgcatc
     1021 atgcagtgaa gtacctccaa tccagttttg gctagttctg aattctccgg gtaatttatg
     1081 ttcacccctt acgctttgca gcagtatttt atgcgtttct tttagtaagc gagaagaaat
     1141 gggcagtgat tttagttctt caatagcatt atttaatgcc ttgatgtaat tatttacttc
     1201 ctgccagtcg tctctacgtt cgggatgaat ttcttcaacc ggcaaaaggg cttcatccat
     1261 attggttttg gtgccttcaa tatggcttga aaccaccgct tctttggtta catgcaattg
     1321 aataaacaaa tcaatattgg gaacgagccg tgcataagaa tttaattcac ccaacttaat
     1381 agctgctttt tccaataaca gattcaaggc cggcgttttc caagtccatt cgtcgttaat
     1441 tgtactagga acaaaatatt tgtaccctaa atttttctca aaatgtccgg cttgatattt
     1501 ttcaagttca atcatatttt tatcgataag gaaaatataa atatcgttat tttactttta
     1561 gcgataaaag taaaatatag ctgcctttat tttacttttc atccatcaat ttgatttttg
     1621 ataaattttc cagaatgatt tcgttcaatc gtgcttcttc tttcagctgc tcttccagtt
     1681 cggctttcag ggcggtg
//
LOCUS       DAOSIB010000151         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_61730_length_1693_cov_12.561661, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000151 DAOSIB010000000
VERSION     DAOSIB010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61730_length_1693_cov_12.561661"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PLD44_08765"
     CDS             complement(<1..470)
                     /locus_tag="PLD44_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOF99454.1"
                     /translation="MSFQEEIRRRRTFAIISHPDAGKTTLTEKLLLFGGAIHVAGAVK
                     SNKIRKTATSDWMEIEKQRGISVATSVMGFDYQDYKINILDTPGHQDFAEDTYRTLTA
                     VNSVIIVIDNAKGVEEQTRKLMNVCRMRKTPVMIFVNKMDREGKDPFDLLDELES"
     gene            complement(844..1260)
                     /locus_tag="PLD44_08770"
     CDS             complement(844..1260)
                     /locus_tag="PLD44_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99455.1"
                     /translation="MKKILILMVSALFLISVAGHAQNQKNVSDKEGKKSEMRQNASQR
                     MSAESRAEYWAKELNLTNEEKAKIAELFKKQDETRMQHQAEMQKSRDEHRAFMESQRK
                     ANDAEMEKIIGKEKFQKYDSIRMARMQTMRNRMRNR"
BASE COUNT          499 a          331 c          244 g          619 t
ORIGIN      
        1 gattccagct catcgagcaa atcgaatggg tctttgcctt cacgatccat tttattgaca
       61 aaaatcatga ccggcgtttt gcgcatccgg cacacattca ttaatttacg cgtttgctcc
      121 tctacacctt tggcgttgtc aatcacaata atcacgctgt tgacggccgt aagcgttcgg
      181 taagtatctt cggcaaaatc ctgatgaccg ggtgtatcca aaatattaat tttgtaatcc
      241 tgataatcga atcccattac ggaggtagct actgaaattc cacgctgttt ttctatttcc
      301 atccagtctg aagttgccgt ttttctgatt ttattcgatt tcacggcacc ggcaacatga
      361 atagctcctc caaaaagcaa aagtttttct gttaaggtgg tttttccggc atcgggatga
      421 ctgatgatgg caaatgttct tctgcgtcga atttcttctt gaaaactcat tttgaatacg
      481 atagataaat tctttattac ttgtttttag cctgcaaagg tacaactttt tgaaaaattt
      541 tcaatctgct ttaaaaggaa taaattttct attggttgaa aaaatttccc caaaaaattg
      601 tgttgtatat tatgaaaccc acatgtatta gatacacaaa cacgtattca gacatgtggg
      661 ttccatcaga ccattaaaaa ataaattata ttctgatgaa ttaaaaaaaa gaggctatcc
      721 tattctttag acagcctcgt taccttaaaa attacctata tacttcaaca caatcttttt
      781 tttcaatgtc taacaaattt tgagccaaaa ttcaagattc ttgttaaaaa cttttagaaa
      841 aaattatcga ttcctcattc tgtttctcat tgtctgcatt cttgccatac gaatagaatc
      901 gtatttctga aatttttctt ttccaatgat tttttccatt tcggcatcat ttgcttttcg
      961 ttgactttcc atgaatgccc gatgttcatc tcttgatttt tgcatttctg cctgatgttg
     1021 cattcttgtt tcatcctgtt ttttaaacaa ttctgcaatt ttggcttttt cttcattggt
     1081 aagattcagc tctttagccc aatattctgc acgactttca gctgacattc tttgtgatgc
     1141 attttgtctc atttcacttt ttttcccttc tttgtcagaa acattttttt gattttgagc
     1201 atgacctgcc acacttatca aaaataaagc actaaccatc aaaattaaaa tctttttcat
     1261 aaacttttgt ttttaaaatt cttattttct ttaaatttta taatttgact tatgaatcaa
     1321 gtcaaggttt aaaatcaaaa tattttttat aaaaaattaa caatttatgt ttgctgtaaa
     1381 atttgaaacc tcaatagaga aaaaataatg gttttgtcat acagcgaaca cagtacagtt
     1441 tcctgttcca atccatcatt ctaacaacac atgttgcaac gcaattcaat agggaaacaa
     1501 acgcgcattc ccccaattca gatcatgcgt tccattctat cattgcaata acacttttgg
     1561 atgttgtata tgagacgtag catgctacgt ctttacccgg attaaacgca attcaatggg
     1621 aaaaatatct gtacattttg ctgccgctca attgaatcgt gtggtagaaa tttccaaagt
     1681 aaagcattta ccc
//
LOCUS       DAOSIB010000152         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_65999_length_1615_cov_9.661538, whole genome shotgun sequence.
ACCESSION   DAOSIB010000152 DAOSIB010000000
VERSION     DAOSIB010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65999_length_1615_cov_9.661538"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PLD44_08775"
     CDS             <1..563
                     /locus_tag="PLD44_08775"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOF99456.1"
                     /translation="ENKKPTLLLHVCCAPCSTYVLEYLSDYFTIALYYYNPNIFPKEE
                     YDKRLWTLVKFVRELKSEIRPIIEEANYDPSEFYRNIEGLENEKEGGGRCQKCYALRL
                     EEAARKATEKSLDYFTTTLSISPLKNAQLINDLGAVYENRYGVRYLYADFKKKEGYKR
                     SIELSKQYGLYRQDYCGCEFSMKIKR"
     gene            565..>1615
                     /locus_tag="PLD44_08780"
     CDS             565..>1615
                     /locus_tag="PLD44_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF99457.1"
                     /translation="MKQINKIAFILLVAIGTVACGNRKDKKFEKDLSDYSGIISAISS
                     GAIRRADPIVVVFTKDIVEKELIGKTLEKNPFVFKPEIKGKCSWKSPNTLVFKPEKPL
                     NWNTNYEASLNLEYLLKLPEKLEKKLNFSFFTAKKQLKLSYDELRLDEGGNYFLEVYI
                     NTSDRFDSHEIEQSLIVEYPHEQLSLKWEHNPETFSHTLSIRNIVREKENSELVLNIK
                     PIDNSYKPSISQYSFTIPGTDVFMLSSVKVKESPDQVLEIVFSDLLNMQQDLRGLVSL
                     DEQKSVKYTIDNNVLKIYPSNTLSGEFTLNISRSVENKQGKTLQKNYSQKINFGRIKP
                     AIRLLGKGVIMPQSQG"
BASE COUNT          564 a          285 c          320 g          446 t
ORIGIN      
        1 ctgaaaataa aaaaccaact cttttgcttc atgtatgctg cgcaccttgt agtacctatg
       61 tgttggaata tttaagcgat tattttacga ttgcacttta ttattacaat cccaatatat
      121 ttcctaaaga agaatacgat aaacggcttt ggacattagt aaaatttgtt cgggaattaa
      181 aatctgaaat tcgtccaatc attgaagaag caaattacga tccttccgaa ttttaccgga
      241 atatagaagg tttagaaaac gaaaaagagg gaggaggtcg atgccaaaaa tgttatgctt
      301 tacgcttgga ggaagctgcc cgtaaagcga ccgaaaaatc cctggactat tttaccacaa
      361 cattaagtat cagtcctttg aaaaatgcac aacttattaa tgacttagga gcagtgtacg
      421 aaaatagata tggtgttcgt tatttatatg ccgatttcaa aaagaaagaa ggctacaaac
      481 gatctataga attatccaaa caatacggtt tatacagaca agattattgt ggttgtgagt
      541 tctccatgaa aattaaaaga taaaatgaaa cagattaaca aaattgcttt cattctcctc
      601 gttgcaatcg gaactgtagc ctgcgggaat agaaaagaca agaaatttga aaaagacctg
      661 agtgattatt ccggcattat ttctgccatc agctccgggg cgataagacg ggcagatcct
      721 atcgttgtgg tatttaccaa agacatagtc gaaaaggaat tgataggaaa gacccttgaa
      781 aaaaatcctt tcgtctttaa gcctgaaatc aaaggaaagt gtagttggaa atcacctaat
      841 actttggttt ttaaacccga aaaaccctta aattggaata caaattatga ggcaagcttg
      901 aatttggaat acttgctgaa gttacctgaa aagctggaaa aaaaattgaa tttcagtttt
      961 tttaccgcaa aaaagcaact taagttatct tacgatgagt tacgactgga cgagggaggt
     1021 aattattttc tggaggtata tatcaacaca tcagaccgtt ttgattccca cgagatagag
     1081 cagagcctga ttgttgagta tccgcatgaa cagttaagct tgaaatggga acataatccc
     1141 gaaacattca gccacacctt aagtatacga aacatagtaa gggagaaaga aaacagcgaa
     1201 ttagtgctga atatcaagcc tattgataat agttataaac cttccatcag tcaatattct
     1261 tttaccatac cggggacaga cgtttttatg cttagctcgg tgaaggtaaa agaaagcccc
     1321 gatcaggtgc ttgaaatcgt tttttctgat ctgcttaaca tgcagcagga tttaagagga
     1381 ttggtttccc tggacgaaca aaagagtgtt aagtatacca tcgataacaa cgtccttaaa
     1441 atctatcctt ccaatacctt gtcgggggag tttactctga atatatcccg ttcggtcgaa
     1501 aataaacagg gaaaaacact tcaaaaaaat tacagccaaa aaatcaattt cgggcgtata
     1561 aagccggcca tacgcttgtt gggcaaaggg gtcatcatgc ctcagagtca aggcc
//
LOCUS       DAOSIB010000153         1538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1712
            NODE_70782_length_1538_cov_14.438301, whole genome shotgun
            sequence.
ACCESSION   DAOSIB010000153 DAOSIB010000000
VERSION     DAOSIB010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863241
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,758
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,706
            CDSs (with protein)               :: 1,706
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 13 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70782_length_1538_cov_14.438301"
                     /isolate="AMR_MDS_1712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..1146)
                     /locus_tag="PLD44_08785"
     CDS             complement(73..1146)
                     /locus_tag="PLD44_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HOF99458.1"
                     /translation="MKERKAIIGKFIAKGMKAEKASMMAGMSKSSYYYRPRGGKPGRP
                     SSTHCLKDNVLLVSNEEVVEDIRTFLSGEFMENGYLKVCHELKDMGYEIGKNKTYRLM
                     KENRLLLPPPQKAKREYVQYTQPLPSEPFEKIEMDIKFIYIRGQHKNALLLTFLDTFT
                     RLPLGWELQYSIRHQTVAALFDRVIENWLQPYHPCFEEKVKVCLRNDNDSRFVAKDLQ
                     NYLKSNFVDQEFILPATPQQNAHIESFHSVVEELVCKKYEFEDIHQAREILDRFFYTY
                     TFRRTINSILYLPPAVFFHEWLEGNIGVSNKKKGNRTKQTFFFIGQRPKWLSVPIEEF
                     YNRLNDKVTKKDDIFVEPSLIES"
     gene            complement(1143..1436)
                     /locus_tag="PLD44_08790"
     CDS             complement(1143..1436)
                     /locus_tag="PLD44_08790"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOF99459.1"
                     /translation="MKRTWNLEEKVSILKEAETNGVVETCRKHGIYATTYYEWKKKYS
                     EGGESALLPGYAKRERKDIKKLEKENERLKKLLAEKELEVEIKTELLKKKMEQ"
BASE COUNT          397 a          310 c          274 g          557 t
ORIGIN      
        1 ttttgtttta gctcattatt aattcccttt ttattgcttc ttgaataata aaagtggctt
       61 aaccccctat tgttaggact caattagtga tggttcaaca aatatatcat ctttctttgt
      121 aactttgtcg ttcaggcgat tataaaattc ttcaatcggg acagagagcc attttggcct
      181 ctgtccgatg aagaaaaaag tttgttttgt tctgttccct tttttcttgt ttgatactcc
      241 gatattccct tccaaccact cgtggaaaaa cactgccgga ggtaagtaaa ggattgaatt
      301 gatagttctt ctgaaagtat aggtataaaa gaatctatcc agaatctctc ttgcttgatg
      361 aatatcttca aattcatatt ttttgcatac cagttcttcc acaacactgt gaaaactctc
      421 gatgtgggca ttctgttgcg gggttgcggg cagaataaat tcctgatcca caaaattact
      481 tttcaaataa ttttggagat cctttgccac aaaccttgaa tcgttgtcgt tacgtaaaca
      541 tacttttacc ttttcttcga aacagggatg gtagggttga agccaatttt cgattactct
      601 gtcaaaaagg gcggcaactg tttgatggcg gatggaatat tgaagctccc atcccaaagg
      661 taaacgggta aaggtatcca ggaaggtaag caagagggca tttttatgtt gtcctcgtat
      721 gtaaataaat ttgatatcca tttctatttt ttcgaatggt tctgaaggca agggctgagt
      781 atattgtaca tattcgcgtt tggctttttg aggcggagga agcaacagac gattttcttt
      841 cattaatcgg taggtcttgt ttttaccgat ctcatagccc atgtctttaa gctcatgaca
      901 aacctttaaa taaccatttt ccataaattc tccacttaga aaagtgcgaa tatcttccac
      961 cacttcttca ttagaaacca acaatacatt gtccttcaaa caatgggtag aagaaggtct
     1021 gcctggtttc cctccacggg gtcgatagta atagctgctt ttactcattc ccgccatcat
     1081 cgatgccttt tctgctttca ttcctttagc aataaacttc ccgattatcg ctttgcgttc
     1141 tttcattgtt ccatcttttt ttttaaaagt tccgtcttga tttccacctc aagttctttc
     1201 tcagcaagca gctttttgag ccgttcgttc tctttttcaa gctttttgat gtcctttctt
     1261 tcccgtttgg cataacccgg caaaagagcc gactctccgc cttcgctata tttctttttc
     1321 cattcataat aggttgttgc atagataccg tgtttgcgac aggtttccac aacaccgttt
     1381 gtttctgctt ccttaagaat cgaaactttt tcttctaaat tccatgttct tttcatctcg
     1441 taaaattagt gttttttatc actaatgaaa tggtctaatg agactggaca atatttttat
     1501 caaagttaat ttattatgtt gaaaagaggg ggtctggg
//
