LOCUS       DAOSID010000001        42627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_1049_length_42627_cov_15.026543, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000001 DAOSID010000000
VERSION     DAOSID010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 42627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42627
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1049_length_42627_cov_15.026543"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PK674_00005"
     CDS             complement(<1..448)
                     /locus_tag="PK674_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14961.1"
                     /translation="MKILDIGNKINKKNLKPPAQFNTETMMPISEIKNDIIILKDGGL
                     RSILKVEGINLDLKNFEDQEIVLEQYKKFLNGLGFPIQIIVRNNYLDLSGYLSYISKN
                     LTKIINPTLKQTGESYYKFLENIDNKQGLIYEKSFYIVVPYYIGEKD"
     gene            complement(489..1016)
                     /locus_tag="PK674_00010"
     CDS             complement(489..1016)
                     /locus_tag="PK674_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14962.1"
                     /translation="MKKIKLLILFTTIFTTIGFSVNANYDFPYESTDNPPVGSEYETD
                     IGKIIKQDNVEQEDSALNKLLALFKLSNQPRYEAGTSKAIYYAKMIVNLMLSLVSFIA
                     LIMVIYAFYMIFFLKDETGITKAKQILKGVAIALIIMGLSWFIVSMIFWIQSNSANNT
                     NNYNNPNNIINNTSS"
     gene            complement(1042..1569)
                     /locus_tag="PK674_00015"
     CDS             complement(1042..1569)
                     /locus_tag="PK674_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14963.1"
                     /translation="MKKNICYILSILFIANSVLLLDSTFGSFNLGNTNTILNTNIEAT
                     IDTNIGGSTIGNPIREGVRQIIHSDDGSYVLTGIADSDVEITEHDTAFNRILEITKRI
                     INRALGLLSLVALIYLIVHGFIILTATGDDTRYKKGMKGIRNATIALGGIGLSWFIVS
                     FIIRIIQNITSSSNL"
     gene            1765..2754
                     /locus_tag="PK674_00020"
     CDS             1765..2754
                     /locus_tag="PK674_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14964.1"
                     /translation="MKKNTFIFFSLYFFVLQPVFAGLLRGDYKFFCLAGINIFIVLII
                     GYFIKNQDNAQKDIRNREDALGFSEKIHYIQKPQNRKFVHHFWFFIIAVLCGFIIWFW
                     MPNMDILLQIFLVTLFSFFVFIIFGLLFKFRSFRVWESKLYFVVLFLSFLGYVLGYFN
                     IDIIYNLNKPINSSLSGDVFISGYDVNSFVPVDADLSESEISSGAIEVGESSNIDLAQ
                     NASFADAIKHLLDQNSVVLLTNQNVKFNYISYNHVDYPYYRTAYGLGMIGKNLNPSKS
                     LLCETYVVMQGLVEKWNIPSYSDIKQAYWNYAKNNNKLPSCSYGKFLTLADLK"
     gene            3046..5625
                     /locus_tag="PK674_00025"
     CDS             3046..5625
                     /locus_tag="PK674_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14965.1"
                     /translation="MKNTLKFIGNIFKKKMMKRLLGIIILLGVSGMFLANNKVVADPQ
                     IEASKTTNKTEEDKFKNMSEVNEVFTYISNAVYALLWPVLFITGTALDNRLVYGSFLH
                     LDASLWALWNIMKNFANFALGFLVLFAIVRNIFTGPFGGKTGEKRGPISIIKKTLIAG
                     VLIQASWFLVAAVVDLSTILTYSIGGLPITVSQNNPQQKDQPILGVKAVINTKGPGGN
                     NKMSLQYYNTYGDVNISPCYTTNKIYGLTGTYIVGRKQIRVNAEQKFISSYCTLGGWP
                     YHYKENEDYLNFSASNEEYTIGLQEYFEQSPHDPGHYLGLIDECKIIPVDSSKLSESC
                     TGYGFLTKDDAFFKNVSENNKIKYTLTNILEKSKGFVGPFITIYSSLLNFSDLAEPAS
                     GSSIVGDFLDLIIKLFFAFVLFIPLLVLAIVLVIRIGLLRLIIAASPVLVLLAVFKQE
                     LNIKGGGEGIMAYFDGAQIVKLVFAPVFIVFAISMSMIFLSALNNRVDKHSGLSDEQF
                     EKLLITKNENSFSFLGLMEIELDMERINKGMDMLSWFITMFFATGIMRFFLFFAIRMT
                     KIGDKIGKGFQESTQNLVKNLPIIPIGGSGVGIKAAKEGLDKLGSVVPDRLNRQQTDH
                     LKKRFPWLYPGVADASRGFDKIRKPELEQILADIRSIGINETFEKHKPTLSSAEIATK
                     DGLLRAYNDYVLKNMDKIETIVAKPEETISSDGIATYNVENLLIDTVEEYLNSDKGKD
                     LAEKAIGKNIITYDGVRVVVNIGNVLEPKYKLLTEQEYDKKYLFDVTDSNGNFVEEKL
                     RQIIEDQIKDDKQPDEMNESDWNKKRQDRINTDLEAHKKYAKERLESSPKKKEPNEPG
                     GQK"
     gene            5655..7499
                     /locus_tag="PK674_00030"
     CDS             5655..7499
                     /locus_tag="PK674_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14966.1"
                     /translation="MKLVKKLIAQMFTKKSIKIWSVFLIFVFLFISQTYASNETLDTT
                     VEYMKIIMDLLSRVWIILANLAGALMTNNILYGSFLHLDASLWALWNIMKNFANFALG
                     FFVLFAIVRNIVSGPFGKDAGERKPIKVIQKTLVAGVLIQASWFLMGAVVDVSTIMTS
                     AIGAFPSQFIAGNSEFQGATLNATKLISKNNKIVINPKEKDIVRFETNAGALQTEEDT
                     KSMLDTILPSHNSVSGPLMFLGISVFGFNDYEAFGTAGDPNSAITDWGDLFLSIGLSG
                     AILVFFTVMMFFIFLFNLFRLIILWIIIPLLPIIILLKVFKLADKLGGSGKGLDLGKL
                     MDIKTILKLIFKPVLLVGALSLILVVLVLIKNIIAGNKTHSITFEDQGNIQIISQKEG
                     SSELYTSTMKSDGMFDFSMTGVKDTFADIIVYLFGLFLIFLLVKFSLKLDTGISFIDN
                     ALNKTFESLENTLKTLPIVPVGGGAVGISAIQEAFSSGNVSGMMTRAVGIDIAKQTDA
                     INRILGLEGSFDSLHDGMSRDDFISRAAEIANQLGYNRNTLVNNQQFMSKFDSWNKYN
                     TKYNRQAIGIDDILKKMKGVDESSSEPTQSPTPSPTQSPTPTQPAPTK"
     gene            7661..8473
                     /locus_tag="PK674_00035"
     CDS             7661..8473
                     /locus_tag="PK674_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14967.1"
                     /translation="MKKVFLGLLAFLFVGSVFAYTPTANDVSNLNNLKFQLSGLIENN
                     NINLRDFYNQLRILQEDYSKDARLNYMLGNLKDHLYHNFFAQKTHAKITSQSGKQEFL
                     DQYLSGISDDIQNSLHNCTGWYNTLDDISFAYDFPTALTIATWYRESTCAYHLPSNKN
                     GPFQIISRDYGTGEITQNKFIETVVDFIEFTKSKFSRHSGLTGKLNYTDFDLAGINNF
                     AGLYNGGKKQGNIIVPNVPKYLYDGYGDAFSGAKKYGIFPQFLKVLERELSQ"
     gene            8585..11515
                     /locus_tag="PK674_00040"
     CDS             8585..11515
                     /locus_tag="PK674_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOG14968.1"
                     /translation="MSKQKKIDMTQYSNEMVFQGVSTHNLKKIDLILPKNKVIVVTGV
                     SGSGKSSFAFDTVYKEGQFRYIESLSSYLRQFFNLGTRPEIEHCSGLSPAIAIEQNKK
                     AGNSRSTVGTLTEIDDYMRLLFAKLGDSYCYSCGKEIKPSSVEAIMNEIKKDYTNKRI
                     FLLKEVGEIKAMDLLFKFVKKNRNKVEKGVGFTRYLLIGENKDPVEYFYLEDPNVPKD
                     YFPLKVYGIYDRITVESSKIDRLKEDVIKILGETEKFGLYIDDVGNKKSGNNKTLPLI
                     QRFTDKMYCPDCNVTYPEFTTQHFSPNRQEGACSECHGIGEILQVDLDKVIDQYSSYS
                     SAILPWRDSTFGQIVLGKLAEKYSIDTDKLRKDLPERFQHVVIYGDEEVIRVQTGGKY
                     SSVKYQGIESILKDQYVKGLLTVDFQAMLNMRSCPSCNGKKLRIESLNVFISLGKNKY
                     NIADLQKFTLDHLLEFFQEYISKSKKSNLLVDRITQPLIDRLQTIQDLGLGYIDLSRT
                     IDTLSGGEIQRLRLAKQLGNKLTGITYVLDEPTIGLSQREIDKTIKAIRNLQLMGNTV
                     IVVEHNESFIKAADQVVEIGPGAGDFGGNIMFNGTYEDFLKSDTLTAKYLTGKLKVKA
                     NFNHRKGDKFISIKKASKHNLKNIDVKIRLGGFTVITGASGAGKTTLMYSTLYRFLNE
                     KQKFIQSFIRLQLLKKGMSWEEIISAPIIQKELYDHYKNLALQEFYQDLAVDTIIGHQ
                     EIKNTIYVDQSNIGKTPRSCPATFIGTFDKIRTIFAGTTQAKYLGFSSSYFSFNSEKG
                     ACPACNGYGYKKVELQFLPDTYVPCDLCKGDRYKSEILEIKRKGKNIAEILNMYVSDA
                     LELFSEIDIIREELQLMCDIGLGYLRMGQAAHTLSGGESQRLKLVKHLLKSYTGHTVY
                     FLDEPTVGLHPQDIEKLLKVLEKFLEAGDTILMIEHDKNIVQFADDIIELDDGKVVES
                     PLSS"
     gene            complement(11779..13098)
                     /locus_tag="PK674_00045"
     CDS             complement(11779..13098)
                     /locus_tag="PK674_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14969.1"
                     /translation="MSKDFQKILILGLISIVISSLALYSFRDIKFKNTKKMLEENQKE
                     QEILQEYQNLLSDPNEITQPEIQKNLAKTGDLTIILPSFFGNAGFDKIVQELNNKEIK
                     INFEYIESISKYRKDILSGLKIDIVYLLPTNRIKGMELEIISINENPKVYFHSIFNNL
                     LGINDNTIIPYSIDPAITLRKNGINIGNNRESLLTFSAIWVQNKVHSMPILWGIGKND
                     IRFLERGEEPFVNYFDILFLNLEQIKENHNVAELKNMLDVEKLSLDYSYNFAKLKLLY
                     ESIGKKNENCKTFPAICLMSYNFGDIKFGFLSDLDILEKYFTNNKNSFEISNFNNTIT
                     SYPVKGRVFVVPKGSEKINLANEFFKEYLKEGVNGNENLWGNTLSAITNIYDIQKQED
                     KYKEILINENKFRLMYENINLQEEFVKDSKNINLLKGDYNPELYLKK"
     gene            13444..15582
                     /locus_tag="PK674_00050"
     CDS             13444..15582
                     /locus_tag="PK674_00050"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOG14970.1"
                     /translation="MAVTKKQFQTVDFTTELSGRQISFETGKLAMQADSSIRLQYGNN
                     VLLFTTCMDKDPKEGTDFLPLMIDYRESYSAAGRIAGALYKRREGRASEVATLYARMS
                     DRALRPMFPKGMINSIVISVTPLALDHTMDMDVPNIIGSSVSIMVAGIPFDGPVGAVQ
                     IGYKDGQFLVNPTREELDAGILNLLLAGKKGSINMIECGANEVPEDVLKQAFVLGQKE
                     IDKSCDIQLEFLNKLNIQKQEVTFNKPGEEVISFISDFMTQDKLDSLTGNTKVPFNTL
                     FSQYEKEVLDLAKEKIEDPENEEFTESKVKMGFFEVIKDFIRNRTIDTGIRLDDRDMY
                     TIRPLFCEVDNLPQVHGVGLFWRGDTQVLTTTTLGGPKDYLVYDDMENDNVQQRYFHH
                     YNFPPFSVGEANIIRFVGRREIGHGKLAEKALMPMLPSQEDFPYSIRTVSECMSSGGS
                     TSMGSVCGSTLSLMDAGVPLKKPVAGIAMGLMTKNTDGDNITKYIILNDLQGTEDFTG
                     DMDFKVAGTRDGVTAIQLDTKLKGLTMEIVHETISRAIQGYNEIMDVMLEAMPTHRAQ
                     VKESAPKIRVFQINPDKVKEVIGKGGDVINQIIEDCGGITIDFEDDGTCFLTHPDQAM
                     IEKAEKIIREIATDLEVGQSFEANISRIEDYGVFVDLPKGKKGLCHISDLGQRFEGGL
                     EKHFKIGQTMNVIIKEIDNMGRIKVKRKLG"
     gene            complement(15844..16380)
                     /locus_tag="PK674_00055"
     CDS             complement(15844..16380)
                     /locus_tag="PK674_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HOG14971.1"
                     /translation="MLVAIGSLRKPKLEAVKLAFQDCPYFQGKHIKYEARKTASNVSD
                     TPLSLEEIMLGAKNRVKSLRNEIKNADFYIGLEGGVSKIGEKYFLLGVTYIENTDGVG
                     HYGFSQMIEIPSKIQYELYENKRDLSELINELANKNEIRSNNGTFGELSDDMIARADS
                     FKNSTQTAIAPFFNKFFK"
     gene            complement(16534..16902)
                     /locus_tag="PK674_00060"
     CDS             complement(16534..16902)
                     /locus_tag="PK674_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14972.1"
                     /translation="MLKLEKELKKLGHEVIIPADTNSFLDGQINDWGINPELARKGML
                     NHYNNIKESDAILVCNLDKKGVKGYIGGATLIEMGVACHRNKKIFLLNDIPSENEIRY
                     VQEIKLMQPIIINNQLDLIT"
     gene            complement(17011..18027)
                     /gene="pheS"
                     /locus_tag="PK674_00065"
     CDS             complement(17011..18027)
                     /gene="pheS"
                     /locus_tag="PK674_00065"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOG14973.1"
                     /translation="MDLQQALSELNLVHDKSNLESFFQKYLGKKGLLNEEFSKMKDAS
                     IEEKKVLGAKLSEIKNKLSEEYSQKEAEISIMEINEQLKKDIVDISVNPKKYEGGHFN
                     LLAKTRRDLEKIASNMGFIIDYGHEVVTKYENFASVNIPLSHPATEMHDTIYLQEQSP
                     DGENFVFRTHNSAHQVQDIQKYGVPLKLVSPGRCYRFDDMDATHDVMFQYIEGIYIDK
                     NVSIAHFKNYINTLLSAIIGRTVETRIRPCHFPFVEPGFEIDARYEIIDPKTGETSKS
                     KRIELLGAGMIHPNVLKEGGVDPEQRSGFAFGIGISRLAAIRYGIKDIRYFTNGDLRF
                     IKSF"
     gene            18241..18411
                     /locus_tag="PK674_00070"
     CDS             18241..18411
                     /locus_tag="PK674_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14974.1"
                     /translation="MELILTPEEQQIQSYYDQIPYHNFEHAIHVTKNALRVANKLKEV
                     LLLTDDDIDKIY"
     gene            18501..18893
                     /locus_tag="PK674_00075"
     CDS             18501..18893
                     /locus_tag="PK674_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14975.1"
                     /translation="MKQFYRNMLQKEKIQKIANTIEATKLNFSLYTTEIQKILQAADI
                     LRLGGNYDEFENNTWKIKKEYDLLHDTQLDTTVFLKKQLQILESFLSKQGVINERYFQ
                     KEHFDFYKTFKQNIKTLEEKYLITKRFD"
     gene            19012..19494
                     /locus_tag="PK674_00080"
     CDS             19012..19494
                     /locus_tag="PK674_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14976.1"
                     /translation="MKRQKIFILVGWLHIEKDVVFEVFNEEFENCRCRFISNYHYLQK
                     KFDYWNFHNEKIAAIFVGPVPHKTAAAGDCNSMASELIIANSPTPVFVCKNKARILKF
                     TKTSLRKAISEYKKYSKLKKIAKPRRKVNLTESPGFIQDFFYLFHITYWLFLDFVKSN
                     "
     gene            19578..20249
                     /locus_tag="PK674_00085"
     CDS             19578..20249
                     /locus_tag="PK674_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG14977.1"
                     /translation="MDKQTHNLLINVDNLTRGFGDTPELLFDRFKFSLYKGDFTVLMG
                     RSGKGKTTFSKFLTGKLKAPRKSIYYKMSDLASFSDKEIQAYRRELGLIFQDSKLLKS
                     LSVRDNIIYPLKIYGIGEATIEAKYARVRRELDLINIEDREIARLSGGERQLVNMARA
                     IIHDPEFIIADEPTGNLDWENTQKVADILIKFHREGKTILLLTHDIHLLNYIKNSVNI
                     NLFKL"
     gene            20264..20725
                     /locus_tag="PK674_00090"
     CDS             20264..20725
                     /locus_tag="PK674_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14978.1"
                     /translation="MNKKDLLTEQIIDEISDMDIAGYLKEEIEKILGYFHYYKLKNIH
                     KNIDFLKDYLSFLDQKNYKQMGLLQCDGDMEIEVTGHDLYYYISQKGLDFVGEKKIKQ
                     KIWQNDYSLNSIMLGIKGFLLLTMDEFCNASPVRLSISKLVWDKETATNKT"
     gene            20859..21119
                     /locus_tag="PK674_00095"
     CDS             20859..21119
                     /locus_tag="PK674_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14979.1"
                     /translation="MAGRNSNRFGEQTFKNLVCLADNRIQHESVRRSLSLKQTVELIE
                     VLLDEEISPNASISELSQYAQRALDTLEYCKKDIAKVRGQYK"
     gene            complement(21214..22125)
                     /locus_tag="PK674_00100"
     CDS             complement(21214..22125)
                     /locus_tag="PK674_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOG14980.1"
                     /translation="MKLKEITKTLDKFFQVNALYEDPSMKNFVPMVYDSIGFDRKNFF
                     EKDFCTRFNGLMIRGGEEVKTIFLSVFPSKEVLENFIKKSQTGDLLFLHHPIFMSSGD
                     PKGNVGTFFQPIKPEILEKIKDKQLSIYTCHAPMDTNNTIGTGVAIIEALGGKMIKEF
                     FPFANGFAGKICEVAPISTQELIKKCEKIFRIPYCDIGGINKKEITKIGIVPGSGDNV
                     AYMKEAEELGVDCYISGEIFNHVDTDRGRIKLQEVKEFEKTTTLSLIGVSHAASEYLV
                     MPLQMKKYVENVLKKNVILLPMSKRRI"
     gene            22333..23661
                     /locus_tag="PK674_00105"
     CDS             22333..23661
                     /locus_tag="PK674_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="metallophosphoesterase"
                     /protein_id="HOG14981.1"
                     /translation="MYFILQKTWMLLIGDLHINSRIKDKIINELKSYISSSSEKNIIF
                     LGDYVYHFSYDRGALLSLYTLFLELYSQGKNVYILSGNHDWLGSTFVFEEAKMTYEMI
                     TKNIFSDQKGGKIEFVTSPKVENIEGEDVLFLPYYLNTQKLEPLFGNVGVEYTEIENT
                     IKSLLQSKKKNEQISAHINQVLLDYIKKYPKLTVIHHYYIEGVNFPGQRARFYFNDIA
                     LSHLFCDLENIKMISSHLHQGFVYKNYLCTGSVRNTSPLEVNHVKTLFQYSHNSIKGK
                     MIFISPYFLIDSSSLGVRNNELLFSVDGESGGEMKQLDEVSLDQFILDTIEQNKKNYF
                     GSEHWKVEFDSEFHLNKKDISVSLKVDDVDYDKIFEYMDENLYKKLKDFKLKKHVVVS
                     KDLSNKLDIEGKNLSQGFSDRKNLLKDYLKAKYPEDNEKYLDFLRKEKIL"
     gene            23916..24899
                     /locus_tag="PK674_00110"
     CDS             23916..24899
                     /locus_tag="PK674_00110"
                     /inference="COORDINATES: protein motif:HMM:NF020477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF1846 family protein"
                     /protein_id="HOG14982.1"
                     /translation="MVVNSEIISHKRHGFDGEKYIEMQKNQILDRLANFSGKLYLEVG
                     GKFLRDPHASRVLPGFLIDSKQTIFYDLRDKIEVLYCVNAEDIISNKKLADQNLDFSE
                     YVFHNLTVIERILGVKPIIVINKIDIANMFDLVLDFERRFQKKQYRVFERYKISSYPY
                     NVKEILSDNGFGSDDHIPTEKNLVLVTGAGHNSGKLSTCLGQIYLDNEIDIKSGYAKF
                     ELFPLKNKGLEHPVNLAYELAVKDSGNKLVLDTFHKEEYGQNSVVNQKDLDNFEATMD
                     IIRNIANHKNYMIKYKSPTDMVINTTSFCIIDEESVKKASLEEIERRQSAE"
     gene            complement(25036..25458)
                     /locus_tag="PK674_00115"
     CDS             complement(25036..25458)
                     /locus_tag="PK674_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14983.1"
                     /translation="MDILQLGGLENLYTAIVALFVFLRIISIIWVAKDISARTNSLLL
                     QISSIVLITFFTPIIGIPLYHIIKPIGYKKDKMPWREACVSNSTICINCGTINAKEHK
                     CCIYCGKKLTIKCKECNMEYPHNYRYCPKCGGPNIELK"
     gene            complement(25480..27687)
                     /locus_tag="PK674_00120"
     CDS             complement(25480..27687)
                     /locus_tag="PK674_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="RelA/SpoT family protein"
                     /protein_id="HOG14984.1"
                     /translation="MDINQLDSFLDYQIDSTENLDILVQEIITKAQKYLPENSKEGIL
                     KAYHLAKKAHEGQSRLSGEPYIVHPLKSTIILMQLKPDLESIQTCILHDVIEDTMITQ
                     EEIQKEFGSEVAQLCQGLVKVSKVRYKGEDRDLETIKKTFLAMGKDLRVIFVKLADRI
                     HNLQTLHYHPEANKREKIALETMKIYVPIAKRLGLYQYQVALENACFKILYPEEFERI
                     IGHLKKGYSGSDKYIKKGIFNITKLLLKEGISEFDVKGRTKSPYRIREKMEYRYQTTD
                     ISSVMDLLAFRIIAKDISDCYVILGIIHKHYTPLIKKIKDYIAVPKFNGYRSIHTTIL
                     GMFKFPIEIQIRTKDMDDVAEYGVAAHYAYSESKGPSRVSQKQAEWVRKLQELVNAYT
                     ESDEKDQFKNKLDIELLSKETLLYTPKGDIIELPKGSTVLDFAFYIHTEIGLKFKNAI
                     VNGEIVPITHIPNTGDIVKINTFRYQYTANKHRSSILKTPSAKTKLLKFIKNTQQEEI
                     LDKSIKILNSKLKELGLPNLVAKNSKINQVFEKKDLEKKLIDATENRLLYNEIIKKAY
                     PKEYKEFYQNKSSKTPPKTENISIKNIIIDDDKLINYYLCPECRPIFPQKIIAKTGKD
                     GIKVHNLNCRALKTISPNKLLEAHRNEENKNIYTVISTLKMDKNLDFIKLLEIFTKLQ
                     IEVNSFSIKDDKDDKTKIINLTRTLKTPGQIGMVREQIKKYGHKLSLIKREII"
     gene            28341..29168
                     /locus_tag="PK674_00125"
     CDS             28341..29168
                     /locus_tag="PK674_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14985.1"
                     /translation="MNNQNLIDLAKQKLLDKRISNGFTLFLNENQFPRRQTEIAEIIG
                     RSLIKQLIPYNSSYCLTRKQTQQLALVLGFDLQKDMRIFLQQHGLNDSVDILRSSRKG
                     KTGGQVIAEGLINQFHINLVLNNSRPGHKLTVSRILDFIDGLGFNLYEDIKNKVEHEK
                     STYTSVLMSMFPGGGSVLETFKKRYGESSEYSFLRKQIILELIKDIKFRGITLDIFIS
                     RSGEILQSTAEGDLGLSNMNLYKRLHWILGYPVGFGLSLKVKKEFAEIWKYHSYPYM"
     gene            29265..31004
                     /locus_tag="PK674_00130"
     CDS             29265..31004
                     /locus_tag="PK674_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14986.1"
                     /translation="MHYTSKEVYQSISQQTNDPIVEWKTCRISGAEFPIYKSDLDFYD
                     KISPVFNGVRYQIPTPTLCPEERQRRRLIFRNDRKLYRGRCDLTNKPIITIYKPESGY
                     KVYSQEARWGDNWNALNYGQDYDFSKTFTDQFANLYRSVPKISNLVMLSENCEYNVIM
                     GNSKNCYMCSTTFQSEACSYCYRCVESNYCVDCVNVLRCEGCYETVDSTSCNNVQFGE
                     LCNTCSHSKYLFDCQGCAFCIGCTGLRNKQYHIFNKAYSKEEYQRLSSQLSEDTIITR
                     FNELKKTVIREGMTQVNCTQSFGNNLTDCKNCSFDFNIADATNSKYSYDSNVLIKDSM
                     DVSMIGDKVNFMYECVASGYEGYHSAFSNLCWTNDSLYYCDHCMYCKHCFGCVGLRNQ
                     EYCILNKQYTKEEYEKLVPQIIEKMKIDKEWGEFFDPSISSFAYNETAAHMFLPLSQE
                     EAIEKGYIRLDKEYPVNIPNGIETIAAYDLPLSSDPTSEEILGKAILCEKTGKPFRII
                     KQELEFYRKHNLPLPRKHPDVRHEERIRIRPKRELHLRKCDHCNVQMLSVYDKVYQGK
                     VYCESCYQKEIYG"
     gene            31098..33344
                     /locus_tag="PK674_00135"
     CDS             31098..33344
                     /locus_tag="PK674_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HOG14987.1"
                     /translation="MTHLIRIYHSFDKDKQSFEDFLDTTYETLKGKTFTLGLHYLKGE
                     IFYSLDIDPVNYPAFESLFYSRFNDFQIVADDKKIWNYDKQKTVLGELYLENEGYYPF
                     KMDHESDVIATMFRTFENFDLINDKVSFFVEFTPVDTANFLFYLKTKLKHRRKRKKID
                     LLFFKYLFIHTPQKNWKAEGKTFFKKKFRENLFEIRTFIIAQSDSKASAEGKIQSLFN
                     NFSVFKNHLSNQFLLRKIHHPFPLISQLKRQKSNLSTTLLSSEEVATFFHFPNSPKNE
                     SSLFTVKSKKLALPVGVPSFDYTKDERGEVYAVNFPREANIVGISDYRSITVPVGIYD
                     EDRLRHMYVIGKTGTGKSKFLLNLMINDIQQGKGIGIIDPHGDSIEEVMTYIPSHRQK
                     DVIIFDPTDIDYPFCMNPLDVNPDESKQVLAKGFIDIFKKFFGANWNSKLEHVLRMIF
                     LALLDKKGSTLFDIIRALTDKDFRYDMIESIQDDVVRNFWTNEFAGRSQQFNTEAIMP
                     ILNKVGQLLSIDMLKNIFASSENKLDLRKIMDEQKILLVKLPKGQLQEDIMGFLGAMF
                     VTKLFQTAMGRQSLGKNERKPFFLYVDEFQNFTTDTFSEILSEARKYGLSLIVAHQFI
                     KQIPSNISDALFGNVGTLISFRVSSEDAEYIKQHFQPFLSGYDLANLNMREFYCKLLV
                     QGQVKDPFSLKACWTPDVDINRNLVDELYRWSRKQYARSLSEAKQVVSEQQQDVIKVI
                     EEFVEPIV"
     gene            complement(33624..34439)
                     /locus_tag="PK674_00140"
     CDS             complement(33624..34439)
                     /locus_tag="PK674_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOG14988.1"
                     /translation="MSKNYNIGIIILLGLILILNIANLFKKDARALETLKVGGINNMK
                     KVEQLYSSSKYISQQTMAIDQALSQINMNAENNNLNENILSPEDVIQKLEEITANGVD
                     NGEESARFTILEYSNFGCGYCRRQSVQGVIDNVIEKYPSEVNSIFRSFVSSSQAIKLG
                     EAIECVGELKRNVRHKFIKQLFATDSGLDTDTILSIAGTLGVNKDSLTKCIDSGKYTQ
                     EVNDQTNEGRNLFGISGTPGNVIIDRETGKFVVIPGAYPVEKFVEEIEKLKNN"
     gene            complement(34570..34893)
                     /locus_tag="PK674_00145"
     CDS             complement(34570..34893)
                     /locus_tag="PK674_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14989.1"
                     /translation="MERKNNYLILIILLGIMLILQILSLFKKDARALETLKVGGADNM
                     KKIEQLYNSDEYISQQTIAIDQALSQMDMLGNLSEEELYEMIAGEEGALEEDVEENIV
                     DIELE"
     gene            complement(34922..35347)
                     /locus_tag="PK674_00150"
     CDS             complement(34922..35347)
                     /locus_tag="PK674_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14990.1"
                     /translation="MPVKKKVSTKKSIAKKTVAKASATKAISIKKPIVKKAITKTSTK
                     KVVKPSVTVETKKTNPVHTCCCSTNCEKFTKIIVCILVILNLLFALFFFFRKDPALKL
                     EELKVGGADNMKKVEQLYNSNLYKDQQTSAIDQYLAGQQ"
     gene            complement(35557..36618)
                     /locus_tag="PK674_00155"
     CDS             complement(35557..36618)
                     /locus_tag="PK674_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="O-antigen ligase family protein"
                     /protein_id="HOG14991.1"
                     /translation="MIGIKYGFYYLFIFLTASFIGFSCVKNFKLKDMYWFQRFLLGIV
                     FFGFLRQSVKMGRPELFMSLGYGKFDDFYFGTNPPLYYLTGYEGTTRRQGIFSGPNNY
                     AYFLTAFFPLILLRRGSGIKKIKKVFQNPIGNLDILYVVLRVLAIAMTLSRSAIIGVA
                     LIIILLAKDRIKKNKKIAIGIFGVFILGLIGISFLKKESTINHVNAKVSYIKEIIDNP
                     LGYGLGSSGPAIHHNGSMLPENYFMQLMLDIGTVGFIFWCIVILQMLLVFKRIKNIKP
                     DPKEKTKPGEISNELVYLHRNRFYIGRSVLLVIGLFLHVFEDSMVNYLFFISFGILSG
                     YLSKFTKQENKSLKNLLSK"
     gene            36963..37898
                     /locus_tag="PK674_00160"
     CDS             36963..37898
                     /locus_tag="PK674_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14992.1"
                     /translation="MTNRLTVVIPTRPNIQNIHGIFGSLDVQTFQNFKVILMIDKVLT
                     KEEFEDLKIQSLKGFKNISNKIYFVSNINTDFIPQQGVSYVRNYGIKLVDTEFLNLFD
                     DDELFDSDYLQKTFDIRHEHKNYIQKDFVLTPTLMFRKTGNIQSLGFDYYNFRISRPH
                     AFQFKKDQKIGQIQMYSGNSLFGPTKIFQEILFDEKLDFVYEDLEFTYRIHKAGYPIF
                     VSSELLLYHMERDKNILEHAWVGSTYAAYRKAKHRMIFIKKNGTKLEKIKFYLLGFRA
                     QPLWLSLKVLLYARRDEKFKIIWSIIKGTLDGMKA"
     gene            complement(37895..38893)
                     /locus_tag="PK674_00165"
     CDS             complement(37895..38893)
                     /locus_tag="PK674_00165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOG14993.1"
                     /translation="MELNKQLKIEGKKVLIVGNRSHKNMGDELILLGTIKLLQEQGKE
                     IYISAYNISWLKSFFKQFINVDEIEFLREIPKGFRSFRKFIKDGGRKTIKKYKEMDSV
                     IVGGGEILTEELPNNYRYWLVSIFPCLKKHKNIYLMGGIQVPKKWYNKKLFSFLLKKT
                     NYIYARDLDSVYGLKKYGFENTEFFMDTSFFAYDWNIIKNANHKKQKYVIINVNKNGE
                     RFLENISNDIKDFLSNGYKILYVPVAKGKNSQYNDIQYYKQIKEKHKKHNSSFEVLDW
                     ENDFDNFIKKLAKAELVISTRLHLFLISSFVGTKTKVYPYQKKILKMQDVIKNLID"
     gene            complement(38972..41287)
                     /locus_tag="PK674_00170"
     CDS             complement(38972..41287)
                     /locus_tag="PK674_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14994.1"
                     /translation="MSGFENIKIEKLPGNSNETGINKDQLKDYFDNRFFDDLVNDRVK
                     IKQQGKNYIFEQRDINSSQISDNNEFIKKFYVDKTIFQDYLKEQQKKEEVSLESDLLL
                     TEFFNEMDTQNKLKKTQEGVINKQIQKLDKDVEQLTKIDDIRDNPELLKSEVDNLLNR
                     FEANKKRPRAPKSSENKHSRHRFEKVIKDDISSLKSIKRELNRYTYTTNMDYFADYVD
                     TMKGHKEQLEKVRQMVSTGKNTAEIPAMLDSIKDVKKEVLFQDIAASYNVEYNKMLKD
                     ATLAKLLNKDLEGFQEYLIGVWSGDIEHPEQDKFYEKHKKDFQEIQSINPGLYKEITT
                     KKQKIDNPKKESVSPEYRNAQITRNNRYCSKAGFGAFDQIGNMFSDLVYRGENPEKKE
                     ARAKVGKIGAIVGGVVLGFKFIQSLFAKKGTPGKWTNVALYGGGLLALFNIPKVIDWT
                     KTVFGKDPEKIEEGLGVKNNAEYQRYIKPPIATMETIGGIPIKTLLDLGIIEEKYNKL
                     KLNYNNYENYVKQTNLSDKEQERLIKSMDKIKKDESLLSDGLSLLGINSIKSLEMEAG
                     SDPTKTLLDSPKMAEHFKNTAESPVNTQLAIEGLEPVSAQARYEMTKDYDPNNFGNHK
                     TIEWMKNGLVKLKDDNRNYILEEMLKHPEINLTTKTINGLKNNAGNHIQFQSYEEMFK
                     TAQLTDFIKNNFKGKTAKSPDPDPFHITLLGDIEFDNAKRYEIWNPETKVINSNFYKN
                     TLEKISSTLGKNKESYVKYLNERWNNEGKVS"
     gene            complement(41426..42016)
                     /locus_tag="PK674_00175"
     CDS             complement(41426..42016)
                     /locus_tag="PK674_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14995.1"
                     /translation="MVNEIFSPGEGIDAGESVRQDISKVTEQDIKRVQGDSAKAKQVQ
                     DEIKKTKATNNNIAKFLSFLLKNIKNEELISAIYDTFFKVVDTRTQTIYLRKSINNIV
                     VIGFFAPFFLNEINKFGLNIYFTELLNDKSGNSMVEYIAYIKKLSKKYHDNVPINKDV
                     LLNLLALIMGEFGISKETLSGPGKDKVKKEIEKKLK"
     gene            42236..42325
                     /locus_tag="PK674_00180"
     tRNA            42236..42325
                     /locus_tag="PK674_00180"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42270..42272,aa:Ser,seq:gct)
     gene            42459..42555
                     /locus_tag="PK674_00185"
     tRNA            42459..42555
                     /locus_tag="PK674_00185"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42497..42499,aa:Gly,seq:tca)
BASE COUNT        14549 a         5469 c         6474 g        16135 t
ORIGIN      
        1 catctttttc tcatatataa taaggaacaa ctatatagaa cgatttttca taaattagtc
       61 attgtttatt atcaatattt tctaggaatt tataataaga ttctcaagtt tgttttaatg
      121 tagggtttat gatttttgtt aaatttttgc ttatataaga aagataaccc gataaatcta
      181 aataattatt acgaactata atctgtattg ggaatcatag tcagtttaga aactttttat
      241 actgctcaag tactatttct tggtcttcaa agttttttaa atctaaattt attcattcta
      301 cttttaatat agatctaagt catcagtctt ttaatattat aatatcattt ttgatttctg
      361 agattggcat catagtttca gtattaaatt gagctggggg tttcagattt tttttattta
      421 ttttgtttcc gatatctagt attttcattt gtttgattat ttgttaaaaa ataaaactta
      481 tgttgaattt atgagcttgt attgttaata atattattgg ggttattata gttatttgtg
      541 ttattggcac tatttgattg tatccaaaat atcatactta cgataaacca agacaatccc
      601 attataataa gggcgatagc aactcatttt aaaatttgtt tagcttttgt tataccagtt
      661 tcatctttca aaaagaatat catgtaaaaa gcgtagatta ccatgattaa tgcaatgaaa
      721 ctaacaagag ataacattaa gttcacaatc atttttgcat aatatatagc tttgcttgtt
      781 catgcttcat atcgtggttg gttgcttagt ttaaataagg caagtaattt attgagtgca
      841 ctatcttctt gttctacatt atcttgtttt ataatttttc atatatctgt ctcatactca
      901 cttcatactg gaggattatc cgtagattca taaggaaaat cataattagc attaacacta
      961 aatcatattg tggtaaatat tgttgtaaat aatattaata atttgatttt tttcattttt
     1021 ggtattgata tttaataaaa actaaaggtt tgatgaggat gttatgtttt gaataattcg
     1081 gattataaaa ctaactataa accagctcaa tcatatacct catagtgcaa tcgtggcatt
     1141 tctaattcat ttcattcatt ttttatatct tgtatcgtct catgtagctg ttaatataat
     1201 aaatccatgg acaattaaat atattaatgc aacaagagat aataatcaca atgctcggtt
     1261 gattattctt ttagttattt caagtatcct attgaaagca gtatcgtgtt ccgtgatttc
     1321 aacatcacta tcggctattc atgttaatac atagcttcaa tcatcagaat gaattatttg
     1381 acggactcat tctctaattg gattgccaat agtacttcac ccaatatttg tatctattgt
     1441 ggcttcaatg ttggtgttca aaatagtgtt tgtgtttcat aaattaaaag atcaaaatgt
     1501 gctatccaac aacaaaacac tatttgcaat aaacaggatg cttaatatat aacatatgtt
     1561 ttttttcatt tgtgtaagtt ttatttaaaa atacaatcag atattccaaa tttagtactt
     1621 tttgatgttc tatcttcctt atattttatt caaaaaacta ttttttgcaa attttgttct
     1681 tgaaaattgt aatatcttta ttataaactt aatatttata taaaaatatt tgactttttt
     1741 atcattttac attcaaatta aaaaatgaaa aaaaacacct ttattttctt tagtttgtat
     1801 ttctttgtac ttcagccagt ttttgcgtga cttttacgat gagattataa gtttttttgt
     1861 ttggctggga taaatatctt tattgtttta ataatttgat attttatcaa aaatcaagat
     1921 aatgcacaaa aagatattag aaacagagaa gatgctttag gtttttctga aaagattcat
     1981 tatatacaaa aaccgcaaaa caggaagttt gtacaccatt tttggttttt tattatagct
     2041 gttttatgtg gatttattat ttggttttgg atgccaaata tggatatttt attacaaata
     2101 tttcttgtaa cattattttc tttttttgtt tttattatat ttggcttatt atttaaattt
     2161 agatcattta gagtatggga atctaagttg tattttgtag tgttattttt gtcgttcttg
     2221 tgatatgttt taggatattt caatatagat attatttata atttaaataa accaataaac
     2281 tcaagcttat ctggagatgt gtttatatct tgatatgatg taaattcttt tgttcctgta
     2341 gatgctgatt tgagtgaaag tgaaataagt tctggtgcta ttgaagtagg ggagtcttca
     2401 aatatagatc tggcacaaaa tgcaagtttt gctgatgcta taaagcattt attggaccaa
     2461 aattctgtcg ttttattaac aaatcaaaat gtaaaattta attatatttc atataatcat
     2521 gtggattatc cttattatag gacagcgtat tgattgggga tgataggtaa aaatctaaat
     2581 ccatctaaat cattactttg tgaaacttat gttgtgatgc agtgactagt agaaaaatgg
     2641 aatatccctt cttattctga tattaaacaa gcatattgga attatgcaaa aaataataat
     2701 aagcttccat cttgttctta ttgaaaattt cttactttag cagacttaaa gtagtttata
     2761 aagttcataa agtttataaa gttcgtaaag ttcataaagt ttataaagtt cgtaaagttc
     2821 ataaagttcg taaagttcat aaagttataa agttcataat ttagtaataa ctttacaaac
     2881 ttgttacttt caactttaca aactttcaac tttcaactat attatatatg ttttttgctt
     2941 ttttggtttg ttttattgag ctaatattac caatattttg aatatatttt ttttaatatt
     3001 ataattatgt atgaattatt tagcaaacta atacataaat tataaatgaa aaatacactt
     3061 aaatttattg gtaatatatt caaaaaaaag atgatgaaaa ggcttttgtg aattattatt
     3121 ctacttggag tttcaggtat gtttttggca aataacaagg ttgttgctga tccacaaatt
     3181 gaagcgagta aaactactaa caaaacagaa gaagataaat ttaaaaatat gtctgaggta
     3241 aatgaggtat ttacatatat ttccaatgcc gtatatgcac ttttatggcc agttttattt
     3301 attacttgaa ctgccttaga taacagattg gtgtattgat cttttctaca tttagatgct
     3361 agtttatggg ctttatggaa tataatgaaa aattttgcaa attttgcttt atgattttta
     3421 gtactttttg caatagttag aaatattttt acttgacctt ttgggggaaa aacctgagaa
     3481 aaacgatgac caatttctat cataaaaaag accctaattg caggagtgtt aattcaagca
     3541 agttggtttc ttgttgctgc agttgttgat ttatcaacaa tactcactta ttctatctga
     3601 tgattaccta ttactgtgtc acaaaataat cctcaacaaa aggatcaacc aattctttga
     3661 gtaaaagcag ttataaacac aaaatgaccg ggatgaaaca ataaaatgag cttacaatac
     3721 tataatactt attgagatgt aaatatatct ccatgttaca ctactaataa aatttatggt
     3781 ttaacttgaa catatattgt ttgaaggaaa caaattcggg tgaatgctga acaaaaattt
     3841 atctctagtt attgtacttt atgatgatgg ccatatcatt ataaagaaaa tgaagattat
     3901 ttaaactttt ctgcgagtaa tgaggaatat actatcgggt tacaagaata ttttgaacaa
     3961 tcacctcatg atcctgggca ttatctttga ctgattgatg agtgtaaaat tatacctgta
     4021 gattctagca agctaagtga gtcttgtaca tgatattgat tcttgacaaa agatgatgct
     4081 ttttttaaaa atgtttctga aaataataag ataaaatata cattgacgaa tattttagaa
     4141 aaatctaaat gatttgtagg accttttatt acaatataca gttctttatt gaacttttca
     4201 gatttagcag agccagcttc gtgaagtagt attgtttgag attttttaga tttaattata
     4261 aaattatttt ttgcttttgt attgtttatt cctttgttgg ttttggctat tgttttagtg
     4321 attagaattt gattacttcg acttataatt gcggcttctc ctgtattggt actgctggct
     4381 gtattcaaac aggaattgaa tataaaatga ggtggagaag gaattatggc ttattttgat
     4441 tgagcacaga ttgtgaaact cgtttttgca ccagtattta tagtttttgc aattagtatg
     4501 tctatgatat ttttgtctgc actaaataat agagttgata aacactcttg attaagcgat
     4561 gaacaatttg aaaagctgtt aataacaaaa aatgaaaata gtttctcttt cttatgattg
     4621 atggaaattg aattagatat ggaaagaatt aataagtgaa tggatatgtt gtcgtggttt
     4681 atcaccatgt tttttgcaac atgaattatg cgatttttcc tattttttgc gattaggatg
     4741 acaaaaatat gagataaaat atgaaaatga tttcaagaga gtactcaaaa tttggtgaaa
     4801 aatcttccta ttattcctat tggttgatca ggtgttggaa tcaaggcggc taaagagtga
     4861 ttggataaat tatgatcagt ggttcctgat agactgaata gacaacaaac agatcatctt
     4921 aagaaaagat tcccttggtt gtatcctgga gttgcagatg cgagtagggg ctttgataaa
     4981 ataaggaaac ctgaattaga acaaattttg gctgatataa gaagtatatg aataaatgaa
     5041 acatttgaaa aacataaacc aacccttagt tcagcagaaa tcgctacaaa agactgattg
     5101 ttgagagctt acaatgatta tgttctaaag aatatggata agattgaaac cattgttgct
     5161 aaacctgagg agactataag cagtgatggt attgctacat ataatgttga aaatctcttg
     5221 attgataccg tagaagaata tttaaattca gataaatgaa aagacttggc cgaaaaagct
     5281 atttgaaaaa atattataac atatgacggt gttagagttg tggtaaatat atgaaatgtt
     5341 ttagagccta aatataaatt attaacggaa caagagtatg ataaaaaata tttatttgat
     5401 gttacagatt ccaacggtaa ttttgttgaa gaaaaattaa gacagataat agaagaccaa
     5461 attaaagacg acaaacaacc agatgagatg aatgaaagtg attggaataa aaaaagacaa
     5521 gatagaataa atacggatct tgaagcacat aaaaaatatg caaaagaaag attggaatca
     5581 agtcctaaaa agaaagagcc aaatgagcct gggggacaaa aataaaatga gtaatttaat
     5641 ttaatatatt tataatgaaa ttggtcaaaa aattgatagc acaaatgttt actaaaaaga
     5701 gcattaagat atggtcggtg tttttaattt ttgtattttt atttatttca caaacttatg
     5761 ctagcaatga aacgctagat actactgtag aatatatgaa gataattatg gatctattat
     5821 cgcgagtatg gataatatta gcaaatttag ctggtgcttt aatgacaaat aatattttat
     5881 attgatcgtt cttacatttg gatgctagtt tgtgggcatt atggaatata atgaagaact
     5941 ttgcaaattt tgctttatga ttttttgtat tgtttgcaat agttagaaat attgtttctg
     6001 gtccttttgg caaagatgcg ggagagcgga aaccaataaa ggtaatccaa aaaaccttag
     6061 tcgcaggtgt tttaattcaa gcaagttggt ttttgatgtg agctgtagtt gatgtatcta
     6121 ccattatgac ttccgctatt tgagcattcc cttctcagtt tatagcatga aattcagagt
     6181 ttcagtgagc aacactaaat gcaactaaat taatatctaa aaataataaa attgtaatta
     6241 atcctaagga aaaagatata gtgaggtttg aaacaaatgc aggtgcacta cagacggaag
     6301 aggatactaa atcaatgcta gacactattt tacctagtca taattctgtt tctggccctt
     6361 taatgttttt gtgaatttct gttttttgat ttaatgatta tgaagcattt tgaacagcag
     6421 gtgatcctaa tagtgctatt acggattggt gagatttatt cttatcaata tgactgtcgg
     6481 gagcgatatt agtttttttt acggttatga tgttttttat ctttttgttt aatctgttta
     6541 gattaattat attatggatt attattccac tcttacctat aataatttta ttaaaagtgt
     6601 ttaaattagc tgataaatta tgaggatcat gaaagggatt agacttatga aaacttatgg
     6661 atataaaaac aatattgaaa cttatattca aaccagttct tttagtatga gctttaagtt
     6721 tgattttagt ggttttggtg cttattaaaa atattatagc atgaaataaa actcatagta
     6781 taacatttga agatcagtga aatatacaaa ttataagtca aaaagaatga agctctgaat
     6841 tatacacttc tacgatgaag tctgattgaa tgtttgattt tagtatgact ggagtaaaag
     6901 atacatttgc tgatattatt gtttatttgt ttgggttgtt tttgatattt ttattggtga
     6961 aattttcttt aaaattggat acatgaatat catttataga taatgcttta aataaaactt
     7021 ttgaatctct tgaaaatact ttaaaaacat tgccgattgt tccggtatga tgatgagcag
     7081 tttgaatttc tgctattcaa gaggccttta gctctggtaa tgtgagtggc atgatgacaa
     7141 gagcggtatg aatagatatt gcaaaacaaa cagatgcaat taataggata ttgggattag
     7201 aaggttcatt tgacagttta catgatggta tgagtagaga tgattttatt agtagagcag
     7261 cagagatagc aaatcaactt ggttataata gaaatacttt agtaaacaac caacaattta
     7321 tgtcaaaatt tgatagttgg aataaatata atactaagta taatagacag gcaattggaa
     7381 tagatgatat tctaaagaaa atgaaaggag ttgatgaatc ttcaagtgag ccaacacagt
     7441 caccaacacc gtcaccaaca cagtcaccaa cgccaacaca gccagcacca acaaaatagc
     7501 tcttcaaata ataaaaaaca attaaggcat acttgagttg tttcaaaaaa cggtttataa
     7561 aaccgttttt tactataaaa tattttttct tgcatataaa aaaagtatct gtataataaa
     7621 taatgttaat tgttgttttt tatatataaa tatttttatt atgaaaaaag tatttttatg
     7681 actattggct ttcctttttg ttgggtctgt atttgcctat actcctactg ccaatgatgt
     7741 ttctaattta aataatctaa aatttcaact tagttgattg atagaaaata ataatattaa
     7801 tttacgagat ttttataatc aattaagaat attacaagaa gactattcca aagatgctag
     7861 actaaactat atgcttggaa atctaaaaga tcatctatat cacaattttt tcgcacaaaa
     7921 aacacatgca aaaattactt ctcaatctgg caaacaagaa tttttagatc aatatttgag
     7981 tggtatctct gatgatattc aaaactcatt acataactgt actggttggt acaacacttt
     8041 ggatgatatt agttttgcat acgattttcc tacggctttg actatagcta cttggtacag
     8101 agaatctact tgtgcttatc atttaccaag taataaaaat ggaccattcc aaattatatc
     8161 cagagattat tgaacatgag aaattactca aaataaattt atagaaactg ttgtagattt
     8221 tatcgaattt acaaaaagca aatttagtag acattcgtga ttgaccggta agttaaatta
     8281 tacggatttt gatcttgctt gaataaataa ttttgctggt ctttataatg gatgaaaaaa
     8341 acaatgaaat attattgtac caaatgttcc aaaatatctg tatgattgat atggagatgc
     8401 attttcttga gctaaaaagt atggtatttt cccacaattt ttaaaagtat tagagcgaga
     8461 gttaagtcag tagtaagttc ataagggaag aaagctttca acttttttgt ttttcaattg
     8521 taaattgatt ttgtatcaat aaactgatta attgataaat ttttttaatc aaaactagaa
     8581 aaatatgtca aaacaaaaaa aaatagatat gactcaatat tccaatgaaa tggtttttca
     8641 atgagtgagt acccataatc taaagaaaat tgatcttatt ttaccaaaaa ataaagttat
     8701 tgtagtcact ggagtctctg gaagtggaaa atcctctttt gcttttgaca cggtttacaa
     8761 agaatgacaa tttcgctata tagagtcttt gtctagttat ttaagacagt tttttaattt
     8821 atgaacaagg ccagaaatag aacattgtag tggactttct ccagccatag ctattgagca
     8881 aaataaaaaa gcttgaaata gtaggagtac ggttgggact ttgacagaaa ttgatgatta
     8941 tatgagattg ctttttgcta aattgggaga ttcgtattgt tactcttgtg gcaaagaaat
     9001 taaaccgagt tcagtagaag caattatgaa tgaaatcaaa aaagattata cgaataagag
     9061 aattttttta ttaaaagaag tttgagaaat caaagccatg gatcttttgt ttaaatttgt
     9121 caaaaaaaat agaaataaag tagagaagtg agtcggattt actaggtatt tactgatttg
     9181 agaaaataag gatccagtag aatattttta tttagaagat ccaaatgtac caaaagatta
     9241 tttccctttg aaggtatatg ggatatatga tcgtattaca gtagaatcaa gtaagattga
     9301 tagattaaaa gaagatgtga tcaagatttt atgagaaact gaaaagtttg gactgtatat
     9361 agatgatgtg ggaaataaaa aatcgggaaa taataaaaca ctccctctta tacaacgatt
     9421 tactgataaa atgtattgtc ctgattgtaa tgtaacatat ccagaattta cgactcagca
     9481 tttttctccc aatagacagg aatgagcttg ttcagagtgt catggaatag gagagatttt
     9541 gcaagttgat ttagataagg tgatagatca gtatagttcg tactccagtg ctattttgcc
     9601 ttggagagat agcacttttt gacaaattgt tttagggaag ttggccgaga aatattccat
     9661 agatacagat aaactacgaa aagatttgcc ggagcgattc cagcatgtag taatatattg
     9721 agatgaagaa gtgatcagag ttcaaacttg atgaaagtat tctagtgtta aatatcaatg
     9781 aattgaaagt attttaaaag atcagtatgt caaaggctta ctgaccgttg atttccaagc
     9841 aatgctaaat atgaggtctt gtcctagctg taattgaaaa aaacttagaa tagaaagttt
     9901 gaatgttttt ataagtttat gaaaaaataa atataatatt gcagatttac agaagtttac
     9961 tttagatcat ttactagaat tttttcaaga atatatcagt aaaagtaaaa aatccaattt
    10021 attagtagat agaatcacac aaccattgat agatagacta cagacaatcc aagatttatg
    10081 attgtgatat attgatttga gcaggacaat agatactttg tctggctgag aaatccaaag
    10141 acttagacta gctaaacaac ttgggaataa gcttacttga attacctatg ttttggatga
    10201 accaacgatt tgattaagtc aaagagaaat agacaagaca atcaaggcta tcaggaattt
    10261 acagcttatg gggaatactg tcattgtagt agaacacaat gaaagtttta taaaggcagc
    10321 cgatcaagta gtggaaatat gaccatgagc atgagatttt tgatgaaata ttatgtttaa
    10381 tggtacttat gaagattttt tgaaatcaga tactttgacg gcgaaatatc ttacttgaaa
    10441 actgaaagta aaagctaatt ttaatcatcg taaatgagat aagtttattt ctataaaaaa
    10501 agctagcaaa cataatttaa aaaatataga tgtaaaaatt agacttggtt gatttactgt
    10561 tattacatga gcaagttgag cttgaaaaac tacattaatg tattcaacac tttatagatt
    10621 tttaaatgaa aaacaaaaat ttatccaaag ttttattaga ctacaacttt taaaaaaatg
    10681 aatgagttgg gaagagataa tttcagcccc gattattcaa aaagagcttt atgatcatta
    10741 caaaaattta gctttacagg aattctatca agatttggct gtagacacaa ttattggtca
    10801 tcaggaaatt aaaaacacaa tatatgtaga tcaaagtaat atatgaaaaa ctcctcgttc
    10861 ttgtccagct acttttatag gtacttttga taaaattaga actatttttg cttgaactac
    10921 ccaagcaaag tatcttggtt tttcttcatc atattttagt tttaattctg aaaaatgagc
    10981 atgtccagct tgtaatggat atggctataa aaaagtggaa ttacaatttt taccagatac
    11041 ttatgtacct tgcgatctat gtaaatgaga tagatacaag tcagaaattt tagaaattaa
    11101 gcgaaagtga aaaaatattg cagaaatttt gaatatgtat gtttccgatg ctttagaatt
    11161 attttcggaa atagatatta ttcgtgaaga attacagctt atgtgtgata tatgattatg
    11221 atatttaaga atgtgacaag ctgctcatac tttatcggga tgagaaagtc aaagattaaa
    11281 attagtaaag catttactaa aaagctacac gggtcataca gtatattttt tagatgagcc
    11341 aacagttgga ttacatccac aagatataga aaaattatta aaagttttgg aaaagttttt
    11401 ggaagcttga gacactatac ttatgataga acacgataaa aacattgttc aatttgcaga
    11461 tgatatcatt gaattagatg atggaaaggt agttgagagc ccactaagtt cttaaagtta
    11521 gaaagttcat caagcctgcc ccaccccgcc ctgcagcccc gcaatactgc ggggcggggc
    11581 ggggcgggat tcatcaagtt tgtacatatt aaataagtgt aggagagctt attcttgttg
    11641 tatatccccg ctattgcggg gtaaacctcg ctattgcggg gtaaatccca cttgttcatt
    11701 caggcaaacc aggacataat aatcgctcgg tattacgata aaaatgttct tttgtaataa
    11761 tggctggggc gagctttttt actttttcaa ataaagctct gggttatagt ctcattttaa
    11821 tagatttata tttttactgt ctttaacaaa ttcttcttgt aagttaatgt tttcatacat
    11881 tagcctaaat ttattttcat ttattaatat ttctttatac ttatcttctt gtttttgtat
    11941 gtcataaata tttgtaattg cagacaaagt atttcaccat aaattttcgt ttccatttac
    12001 tcattctttt agatactctt tgaaaaattc attagctaaa tttatttttt cgcttcattt
    12061 gggaacgaca aaaactcgtc atttcacagg gtatgatgta atggtattat taaaattgga
    12121 tatttcaaat gagtttttat tatttgtaaa atatttttct aaaatatcta aatcggataa
    12181 aaatcaaaac tttatatctc aaaaattata agacattagg catatagcag gaaatgtttt
    12241 acaattttca ttttttttgc ctatgctttc atatagtagt ttaagtttag caaaattgta
    12301 actataatcc aaggataatt tttctacatc taacatattt ttcaattctg caacattgtg
    12361 gttttctttt atttgttcca ggtttaaaaa tagaatatca aaataattta caaaaggttc
    12421 ttctcatctt tctaaaaatc ttatatcatt ttttcctatt ccccaaagta ttggcataga
    12481 atgtactttg ttttgcaccc atatagcact aaatgtcagc aagctttctc gattgtttca
    12541 aatatttatt ccattttttc taagtgttat agcaggatct atagaatatg gaattattgt
    12601 attatcattg attcataaaa gattgttgaa aatgctatga aaatatactt ttggattttc
    12661 atttatacta atgatttcca attccattcc ctttatccga tttgttggca ataagtaaac
    12721 gatatctatt tttagacctg ataaaatgtc ttttctatat tttgaaatag attcaatata
    12781 ttcaaaattt attttaattt ctttattgtt caactcttgt acaattttat caaatccagc
    12841 atttcaaaaa aaactaggca atattatcgt taaatctcaa gtttttgcta aatttttttg
    12901 gatttcaggt tgtgttattt catttggatc gcttaataag ttttggtatt cttgtaaaat
    12961 ctcttgttct ttttgatttt cttccaacat tttttttgtg tttttaaact ttatatctct
    13021 aaaactgtat agggccaggg aacttattac gatagaaata agtcataaaa ttagaatttt
    13081 ttggaaatct ttactcatta taatacagga aaataaaagt tttatgtaca gcatgtatct
    13141 tattaacaaa ttttgcttgt atttcaagct gtatctattt agttaatata ataatatgag
    13201 tttacaaaat caagtttgga ttgtggttaa tttaagtagc tatagttttg ttaactgttg
    13261 aaacttttat tttgcaattg ggtttgaaat tactataatt gtagtagggt ttgaaaatat
    13321 agaaatttag tcacttagaa aaaggtttag aagtcaactt attctatatt ttgtttgatc
    13381 ttttttctaa gtttctagtt tctataatca aatttcacat ttattatttt aatactagaa
    13441 attatggcag ttacaaaaaa acaatttcaa acggtagatt ttactactga gctttctggt
    13501 agacaaattt cttttgaaac ttgaaagtta gctatgcaag ctgatagttc aataagactt
    13561 cagtattgaa acaatgtgtt gctgtttact acttgtatgg ataaagatcc aaaagaaggg
    13621 acagattttc ttcctttaat gattgattac agagaaagtt attctgcagc aggtagaata
    13681 gcatgagctt tatacaaaag aagggaatga agagcatcag aagtggctac actttatgct
    13741 aggatgtctg atagagcttt aagaccgatg tttcctaaag gtatgataaa cagcattgtt
    13801 atttcagtta ctcctttggc attagatcat acgatggata tggatgttcc aaacatcatt
    13861 tgatcctcag tatctattat ggtagcagga ataccatttg attgacctgt aggagcagta
    13921 caaatttgat acaaagatgg acagttttta gtgaatccaa ctagagagga gcttgacgca
    13981 gggatattaa atctattact agctggtaaa aaatgatcaa tcaatatgat agaatgtgga
    14041 gccaatgaag ttccagaaga tgtattgaaa caagcatttg ttttatgaca aaaagagata
    14101 gataagtctt gtgatattca attagagttc ttaaacaaac taaatatcca aaaacaagaa
    14161 gttacattca ataaaccatg agaagaagtg atatctttta tttcagattt tatgactcaa
    14221 gataaattag attcattaac atgaaataca aaagttccat tcaatacatt attctctcag
    14281 tatgaaaaag aagttttgga cttggctaaa gaaaaaatag aagatccaga aaacgaagaa
    14341 tttacggaaa gtaaggtgaa aatgtgattt tttgaagtga taaaagattt tattaggaat
    14401 cgtactatag atacatgaat taggcttgat gatagagata tgtacactat taggcctcta
    14461 ttttgtgaag tggataattt gccacaggtt cattgagttg gtcttttttg gaggtgagat
    14521 acacaggttt tgactactac tactttatga tgaccaaaag actatcttgt ttatgatgat
    14581 atggaaaatg ataatgttca acaaagatat ttccatcatt acaatttccc accattttct
    14641 gtgtgagaag ctaatattat tagatttgta tgaagaaggg aaataggaca cggtaaatta
    14701 gcagaaaaag cattgatgcc tatgcttcca tctcaagaag attttcccta tagtattagg
    14761 actgtttcag aatgtatgtc atctggatga tcaacatcta tgggttctgt ttgtggatca
    14821 actttatctt taatggatgc aggagttccc ttgaaaaagc ctgttgcagg aattgctatg
    14881 tgattgatga caaaaaatac tgatggagac aatattacaa aatatattat tttgaatgat
    14941 ttacaatgaa cagaagattt tactggagat atggatttca aagtagctgg tactagagat
    15001 tgagttacag caattcaatt agatacaaaa cttaaatgac ttaccatgga gattgttcat
    15061 gaaacaattt ctagagctat acaatgatac aatgaaatta tggatgttat gttggaagct
    15121 atgccgactc atagagctca agtaaaagaa tcagctccaa aaattagagt attccaaata
    15181 aatccagata aagtaaaaga ggtaattgga aaatgatgag atgtaatcaa tcaaattatt
    15241 gaagactgtt gaggtattac gattgatttt gaggatgatg ggacatgttt tttgacacat
    15301 ccagatcaag ctatgatcga aaaggctgaa aaaattattc gtgagatagc tacagactta
    15361 gaagtatgac aatcttttga agcaaatatt tctagaatag aggattatgg ggtatttgtt
    15421 gatttaccaa aaggtaaaaa atgactttgt catatttctg atttagggca aaggtttgaa
    15481 ggatgattgg aaaagcattt caaaatatga caaactatga atgtaataat caaggaaatt
    15541 gataatatgt gaaggataaa agttaaaaga aagttgggat aattataagt tatttattat
    15601 acgcaaaaag ggaggtcaaa ataccttcct tttttatttg ttttgtgttc tttttaacaa
    15661 gggttgtgac agtttgtcac aagtaggttt actatattcc ccccttgtct tgataatttc
    15721 gatcggtatc attttccttg ttccaagata ccttaatcct aatataggat cataggtaaa
    15781 aattaatatt tcttccataa tttttttaat ttttttgtta atactaggtt tatatatatt
    15841 tttttattta aaaaacttat taaagaatgg tgctatagca gtttgtgtac tatttttgaa
    15901 tgaatctgct ctagcaatca tatcatctga tagctctcca aaagtcccat tattacttct
    15961 aatttcattt ttattcgcta attcattaat taactcactt aaatctcttt tattctcata
    16021 taattcatac tgtattttgc tagggatttc tatcatttga gagaagccat aatgtcaaac
    16081 tcaatctgta ttttctatat atgtcactca aagtaaaaaa tatttttctc aaattttact
    16141 aactcatcat tccaatcata tatagaaatc agcatttttt atttcatttc tcaaactctt
    16201 tactctgttt tttgctcata acattatttc ttccaaactc agtggcgtat ctgaaacatt
    16261 tgatgctgtt tttctggctt catactttat atgttttcat tgaaaatatg gacagtcttg
    16321 aaatgctaat tttactgctt ctaattttgg ttttctaaga gatcaaatag ctacaagcat
    16381 aagttgaaaa gtttatcaat ttaaaagccc gctcattccg tagtcctacc cgtaacttcg
    16441 caacatttca tcataacgga acttgcgtgt gggatttttg aagttaatat tattgattat
    16501 actacacatc accatgaaac taccttaaag gtattatgtg attaaatcaa gttgattatt
    16561 tattatgata ggttgcatta gttttatttc ttgtacatat cttatttcat tctcacttgg
    16621 aatatcattt aataaaaata tttttttgtt cctatgacaa gcgactcaca tttctattaa
    16681 tgttgctcat ccaatatatc atttaactcc tttcttatca agattacaaa ctagtatagc
    16741 atcgctttct tttatattat tataatgatt taacattcat tttctagcta gttctggatt
    16801 tattcaccaa tcattaattt gtcagtccaa aaaagaattt gtatcagcag gtattattac
    16861 ttcatgtcat aattttttaa gttctttttc cagtttcaac atttcatttg caaaggctaa
    16921 actcccacat atagtaatct tcataccatt caatcaaaat ttaaaactat ttttttgttt
    16981 ttttggttaa tgctaagtat atattttttg ttaaaaactt ttaataaatc ttaaatctcc
    17041 gttggtaaaa tatctaatat ctttgattcc atatctaatt gctgctaatc tgcttatccc
    17101 aattccaaaa gcaaaacctg atcgttgttc tggatctact catcattctt ttaatacatt
    17161 tggatgaatc atacctgctc ataaaagctc tattcgttta gacttagaag tttctcaagt
    17221 ttttggatct ataatctcat atcttgcatc tatttcaaaa cctggttcta caaaagggaa
    17281 gtggcaaggt cttattctag tttccactgt tcttcaaatt atagcagaaa gtagagtatt
    17341 gatataattt ttgaaatggg caatacttac atttttgtct atatatattc attcaatata
    17401 ttgaaacata acatcatgag tagcatccat atcatcaaat ctataacatc taccaggaga
    17461 aacgagtttt aatggtactc aatatttttg aatgtcttgt acctgatgtg cagaattgtg
    17521 agttctaaat acaaaatttt ctcaatctgg actttgttct tgcaagtata tagtatcgtg
    17581 catttctgta gcaggatggg ataatggaat atttacagat gcaaagtttt catattttgt
    17641 gactacttca tgaccgtaat ctattataaa tcacatgttg ctcgctattt tttctaggtc
    17701 tctcctagtt tttgctagta agttgaaatg tcatcattca tatttttttg ggtttacaga
    17761 aatatcaact atatcttttt ttagttgttc atttatctcc ataatgctta tttctgcttc
    17821 tttttgggaa tattcttcac ttagtttgtt ttttatttca gataatttag ctcatagtac
    17881 ttttttttct tcaatactag catctttcat tttagaaaat tcttcgttta ataatcattt
    17941 tttacctaaa tatttttgaa aaaatgattc taaattagac ttgtcgtgta ctaaatttaa
    18001 ttctgatagt gcttgctgta gatccatgat ctgttgttaa acaaaaataa atattattat
    18061 atgtacataa aacatattca tacagtaaaa aaacataatc ttttccattt gtttttcaaa
    18121 tgttttttga tttgactttt tgatgtatat gtttatagtg tgttatgttg atagtatttt
    18181 ataatgcaaa ctaaacacaa tacaaataaa aatagtttat ctatataaat gtataaagtg
    18241 atggaactta ttttaactcc agaggaacag caaattcaat catattatga tcagataccg
    18301 tatcataact ttgaacatgc tatacatgtt acaaaaaatg ccttgagagt tgccaataaa
    18361 ttaaaagagg ttcttttgct taccgatgat gatatagata agatatacta agaaaggccg
    18421 cgttatggca tgatgcatga tatgcatatg ataataaagc agaatgattt gaaacaaaag
    18481 aggattactc aatccactta atgaaacagt tttatagaaa tatgcttcaa aaagaaaaaa
    18541 tacaaaaaat tgcaaataca atagaagcaa ccaagttaaa cttttcatta tatacaacgg
    18601 aaatacaaaa aatattacaa gcagctgata tattgcgatt atgaggtaat tatgatgagt
    18661 ttgaaaacaa tacatggaaa atcaaaaaag agtatgattt attgcatgat acacaattgg
    18721 atacaacggt atttctaaaa aagcagttgc agattcttga gtctttcctt tcaaaacagg
    18781 gtgttatcaa cgaaagatat tttcaaaaag aacattttga cttctacaaa acatttaaac
    18841 agaatatcaa aacactagag gaaaaatacc tgataacaaa aagattcgat taaaaaattc
    18901 cgtttgcctt ctttttaata atagtgctat tagaatctaa gataatcttt ttttctgata
    18961 cgaacaaaca agtataataa aacaaataca ataaaacaaa aaaatatgat tatgaaacga
    19021 cagaaaattt ttattttggt cggctggtta catatagaaa aagatgttgt tttcgaggtt
    19081 tttaatgagg agtttgaaaa ctgtagatgt agatttattt caaactatca ttatttacaa
    19141 aaaaaattcg attattggaa tttccataat gaaaaaattg ctgctatttt tgtaggtcct
    19201 gtaccacaca aaactgcagc agctggagat tgtaattcta tggcttctga gctaattatt
    19261 gcgaattctc caactccagt atttgtttgc aagaacaaag ctaggatatt aaaatttacg
    19321 aaaacatctt taagaaaagc tatatcagaa tataaaaaat attcaaaact aaaaaaaata
    19381 gccaaacccc gccgaaaagt taacctaaca gaaagtccgt gatttattca ggactttttt
    19441 tatttattcc atataactta ttggttattt ttagattttg tcaaatcaaa ctaaagtaca
    19501 aaatgatttt tttttgcaat atttcttttt tgctactata attgagtatg attatattta
    19561 ttcataataa actcattatg gacaaacaaa ctcataattt attgataaat gttgataatc
    19621 taactcgagg atttggagat acaccggaac ttttgtttga taggtttaag ttttctttgt
    19681 acaaatgaga ttttacggta ttaatgtgaa gatctggaaa agggaaaacg accttttcta
    19741 agtttttaac atgaaaacta aaagctccaa gaaaatcaat ttattataaa atgtcagatc
    19801 ttgcgtcctt ttcagataaa gaaattcagg cttatagaag agagcttgga ttaattttcc
    19861 aagattctaa attgttaaaa tctttaagtg tcagagataa tattatctat cctttgaaga
    19921 tatattgaat aggggaggct acaatagaag ctaaatatgc tagagttagg agagagttgg
    19981 atttgataaa tattgaggat agagaaatcg ctaggcttag ttgaggggaa agacaattag
    20041 taaatatggc aagagcaatt attcatgatc cggaatttat tatagcagat gagccgacgg
    20101 gaaatttaga ttgggagaac actcaaaaag tggcagatat cctgattaaa ttccatagag
    20161 aatgaaaaac aatacttttg cttacgcatg atatccattt attaaattat attaaaaatt
    20221 cggttaacat taatttattt aaactttaat aaaaaatata gacatgaaca aaaaggattt
    20281 attaacagag caaataattg atgaaatatc tgatatggat atcgctggat acttaaaaga
    20341 agaaattgaa aaaattttgt gatattttca ttattacaaa ttgaaaaata ttcataaaaa
    20401 tatagatttt ctaaaagatt atttatcttt tttggatcaa aaaaattata aacaaatggg
    20461 tttgttgcaa tgtgattgag atatggaaat agaagttaca gggcatgatt tatattatta
    20521 catatctcaa aaatgattag attttgtttg agaaaaaaag attaaacaaa aaatatggca
    20581 aaatgattat agtttaaatt ctataatgtt gggaattaag tgatttttac tgctaactat
    20641 ggatgagttt tgtaatgcta gtcctgtaag attatctatt agcaagctgg tatgggataa
    20701 ggaaacagcg acaaataaaa cctaatatac aataattaag gcaataatac agtgtttcta
    20761 tgaagatgat tttttattgt ctttttttat tgtttttttt gtgttgcttt attgtaaata
    20821 atatgtataa tattttttta ttttaaattt tagaaaccat ggcttgaaga aattctaata
    20881 gatttggaga acaaacattt aaaaatttgg tttgtttggc agacaatagg atccaacatg
    20941 aatcagtaag gcgttcttta tctttaaaac aaacagtaga actaatagag gttcttttag
    21001 atgaggaaat aagcccaaat gcttctattt cagagttatc acaatatgca caaagagcat
    21061 tagatacatt ggagtactgt aaaaaagata ttgcaaaagt tcgtggccaa tacaaataat
    21121 ttatttttga taaggttaat atttatattt tgatggattt tttttggtat tcaaaaattt
    21181 ttgtcgtttt tttgtttgtt caagtttttt attttatatt cgtcgtttgc tcataggaag
    21241 tagtataaca ttttttttta aaacattctc tacatatttt ttcatctgta aaggcattac
    21301 tagatattcg gatgcggcat gtgatactcc aattagtgag agagtggttg ttttttcaaa
    21361 ttcttttacc tcttgtaatt ttattcttca acgatctgtg tctacatgat tgaatatttc
    21421 tcatgatata taacaatcca cacccagttc ttctgcttct ttcatatatg ctacattgtc
    21481 tccacttcat ggtacaatgc cgattttggt tatttctttt ttgtttattc atcctatatc
    21541 gcaataaggt attctaaata ttttttcaca tttctttata agttcttgtg tggaaattgg
    21601 tgctacctca caaatttttc atgcaaatcc atttgcaaat gggaaaaatt cttttatcat
    21661 tttacctcat aatgcctcta ttatcgctac tcctgttcct attgtattgt ttgtgtccat
    21721 tggtgcatga caagtgtata tagaaagctg tttatctttt attttttcta atatttctgg
    21781 ttttattggt tgaaaaaatg ttcatacatt tcattttgga tctcatgatg acataaaaat
    21841 aggatgatga agaaaaagta ggtcaccagt ttgcgatttt tttataaaat tttcaagtac
    21901 ttcttttgaa gggaatactg acaaaaatat tgttttaact tcttctcatc cccttatcat
    21961 taatccattg aatcttgtgc aaaaatcttt ttcaaaaaag ttttttcgat cgaatcctat
    22021 ggaatcgtat accataggaa caaaattttt catggaaggg tcctcatata aagcatttac
    22081 ctgaaaaaat ttatcgagtg tttttgtgat ttcttttagt ttcatgattt tttggggtga
    22141 attgtataaa atatctttac actaagagat agggataatt tattttaaat caatctatta
    22201 ttatataaag cattactcct tcttttttgg aattaaaatt gttggcatat tctttctggg
    22261 cttattctaa tgaattgaaa ttgaggtctt ttttgtattg aaaacatgag ttataatatt
    22321 acatataaaa ttatgtattt tattttacaa aagacttgga tgcttttgat ttgagattta
    22381 catataaatt cgcgtatcaa agataaaatt ataaatgaat tgaaaagtta tatttctagt
    22441 agctcagaaa aaaacattat tttcttagga gattatgttt atcatttttc ttatgatagg
    22501 tgagcattac tgtctcttta tactttgttt ttggagctgt attctcaatg aaaaaatgtt
    22561 tatatacttt ctgggaatca tgattggctt ggaagcactt ttgtatttga agaggctaaa
    22621 atgacctatg agatgattac aaaaaatatt ttctcggatc aaaaatgatg aaaaatagag
    22681 tttgttacaa gtcctaaagt agaaaatata gagtgagaag atgttttgtt tttaccttat
    22741 tatttaaata cccaaaagct tgagccgtta tttggtaatg tttgagttga gtatactgaa
    22801 atagaaaata ccataaaatc tttgctacaa tctaagaaaa aaaacgaaca aattagtgca
    22861 catataaacc aagttttgtt ggattacata aaaaaatatc ccaaacttac tgttatacat
    22921 cattattata tagaatgagt taattttccg tgacaaagag ctaggtttta tttcaacgat
    22981 attgcattgt ctcatttgtt ttgcgattta gaaaatataa aaatgatttc ttcacatttg
    23041 catcaaggtt ttgtttataa aaattatttg tgtactggga gtgttcgaaa cacttctcca
    23101 ttggaagtaa atcatgttaa aacattattt caatattctc acaattctat caaatgaaaa
    23161 atgattttta tcagtcctta ttttttgata gatagctcaa gccttggggt aaggaataat
    23221 gaattattgt tttcagtaga ctgagagagt ggttgagaaa tgaaacaatt ggatgaagta
    23281 tctttagatc agtttattct tgacacgata gagcaaaata aaaaaaacta tttttgatca
    23341 gagcattgga aggtagaatt tgattcggaa tttcatttaa ataaaaaaga tatttccgtt
    23401 tctttgaaag tagacgatgt agattatgat aaaatttttg aatacatgga cgaaaattta
    23461 tacaaaaaat taaaagactt taaattaaaa aaacatgtag tagtttccaa ggacttatct
    23521 aacaaattgg atatagaatg aaaaaacttg tctcaatgat tttctgatcg aaaaaatttg
    23581 ttaaaagatt atttaaaagc aaagtatcca gaagataatg aaaaatattt agattttttg
    23641 agaaaagaga agattctgta aagtttgtaa agttcataaa gtttgtaaag ttcataaagt
    23701 ttgtaaagtt tgtaaagttc atcaagttca taaagtttat aaagttcatc aagtttgtaa
    23761 agtttataaa gttcttaaag tattaataac tttataactt ttaactttat aaacttgcaa
    23821 cttttaacta aacaaaggaa tgtcaagccg tattttttga caaaataatg tattttgggt
    23881 ataatttatt tgtaatttat tttgtttata aaaatatggt agtaaatagc gaaattattt
    23941 ctcataaaag acatgggttt gatggagaaa agtatataga aatgcaaaaa aatcaaatat
    24001 tagacagatt ggcaaatttt tctggaaaac tttatttgga ggtttgatga aaatttttaa
    24061 gagatccaca tgcatctaga gtattacctt gatttttaat agacagtaaa cagacaattt
    24121 tttatgattt aagagataaa atagaagtat tgtattgtgt aaatgcagaa gatattattt
    24181 ccaacaaaaa gttggctgat caaaatttag acttttcgga atatgtgttt cataatttga
    24241 cagttataga aaggatattg tgagtaaaac caattatagt tattaataaa atagacatag
    24301 caaatatgtt tgatttagtt ttagattttg agcgtagatt tcaaaaaaaa caatatagag
    24361 tttttgaaag atataaaata tcatcttatc cttacaatgt gaaagaaatt ttgagcgaca
    24421 acggatttgg ttcagatgat catattccta cagaaaaaaa cctagtctta gtgactggag
    24481 caggacataa tagttgaaaa ttgtccacat gtctatgaca aatttatttg gataatgaga
    24541 tagatattaa atctggttat gcaaaatttg aacttttccc gttaaaaaat aaaggattgg
    24601 aacatcctgt aaatttagct tacgaattgg cagtaaaaga ttcttgaaac aagcttgttt
    24661 tggatacttt tcataaagaa gagtactgac aaaattctgt agtaaatcaa aaagatttag
    24721 ataattttga agctacgatg gatattatca gaaacatagc aaatcataaa aattatatga
    24781 taaaatataa atcacctaca gacatggtaa tcaataccac atctttttgt ataatagatg
    24841 aagaaagtgt aaaaaaggct tctttggaag aaattgaaag aagacaatca gcagaataaa
    24901 acaataaata tagagtttgt acaaatgaaa actggtttat taaactagtt tttattgaaa
    24961 aaggcgaaat attttgtaat tcttttaaat ttgtacttta tagtgaaaga caacaattat
    25021 tttttggtag tatttttatt ttaactcaat attgggtcat ccacactttg ggcaataacg
    25081 ataattatgt ggatattcca tattacattc tttacatttt atggtaagtt ttttcccaca
    25141 ataaatacaa catttatgtt ctttagcatt tattgtacca caatttatac atatagtaga
    25201 attgctaaca caggcttctc tccaaggcat tttgtctttt ttatatcata tcggtttaat
    25261 aatatgatag agtggtattc aaattatagg agtaaaaaaa gttatcaata ctatagagct
    25321 aatttgtaat aaaaggctgt ttgtcctcgc ggaaatatct tttgctaccc aaataatact
    25381 tataattcgt aagaatacaa ataaggctac gatcgctgta taaaggtttt ctagtcatca
    25441 aagttgtaga atgtccattt tagtaattta tgatataaat tatattattt ctctttttat
    25501 taagcttagt ttatgtcaat acttttttat ttgttcacga accattcaaa tttgtcatgg
    25561 ggtttttagt gtccgagtca gatttattat tttggttttg tcgtccttat cgtcttttat
    25621 agaaaaacta tttacttcaa tttgtaattt tgtaaatatt tccaataact tgataaagtc
    25681 caaatttttg tccattttca aagtagatat aactgtataa atatttttat tttcttcatt
    25741 tcgatgtgct tccaataatt tgtttggaga aatggttttg agtgctctac aatttaaatt
    25801 gtgtactttg atcccatctt tcccagtttt cgctattatt ttttgaggga aaattggtct
    25861 acattcaggg cataaatagt aatttattag tttatcatca tctattatta tatttttgat
    25921 gctaatgttt tcggtttttg gtggagtttt actactttta ttttggtaaa actctttgta
    25981 ttcttttgga taagcctttt ttataatttc gttgtacagt agtctgtttt ctgtagcatc
    26041 gatcagcttt ttttctaaat cttttttctc aaaaacttgg tttattttgc tattttttgc
    26101 aacaagattt ggtagaccaa gttcttttaa tttactattt aatattttaa tcgatttatc
    26161 taaaatctct tcttgttggg tgttttttat aaatttaagt aatttggttt tggcactcgg
    26221 agtttttaaa atggaacttc gatgtttgtt tgcagtatat tgatacctaa atgtatttat
    26281 tttgacgata tctcatgtgt tgggaatatg tgtaatagga acaatttctc cgtttactat
    26341 tgcattttta aattttaaac caatttccgt atgtatataa aatgcaaaat ccaatactgt
    26401 gcttcattta ggtaattcaa ttatatctca ttttggtgtg tagagcaagg tttctttact
    26461 caaaagctca atatctagct tgtttttaaa ttgatctttt tcgtcacttt cggtgtatgc
    26521 atttactaat tcttgtaatt tccttaccca ttctgcttgt ttttgactaa ccctgcttgg
    26581 tcattttgat tcactgtatg cataatgagc agcaactcaa tattcagcta catcatccat
    26641 atcttttgtt cttatttgta tttcgatagg aaacttaaac attccaagta ttgtagtatg
    26701 aatgctccta tatccattaa attttgggac agcaatataa tcttttattt tttttattaa
    26761 aggggtatag tgtttgtgga ttattcacag tattacataa caatctgaaa tgtctttggc
    26821 aatgattcta aatgctaata agtccatgac agatgaaata tccgttgtct gatatctata
    26881 ttccattttt tctcggatcc tatatggact ttttgttctt cattttacat cgaattcact
    26941 aattcattct tttagtagga gttttgtgat attaaatatt ccttttttta tatatttatc
    27001 ggaaccagag tatcattttt ttaggtgtca aattatcctt tcaaattctt ctggatatag
    27061 tattttgaaa caggcatttt ccaatgctac ttgatattga tatagcccca atctttttgc
    27121 aattggaaca taaattttca tggtttccaa tgctattttt tctcttttat tagcttctgg
    27181 gtggtaatgt aaagtctgta agttgtggat acgatctgca agtttgacaa aaataactct
    27241 taaatcttta cccatagcta aaaatgtttt ttttatggtt tctaaatctc tgtcttctca
    27301 cttatatctt acttttgaaa cttttactaa tcattggcat aattgagcaa cttcagatca
    27361 aaactctttt tgtatttctt cttgagtaat cattgtatct tctatcacat cgtgcaagat
    27421 acatgtttgg atagattcca gatctggttt taattgcatc aatattattg ttgatttcaa
    27481 aggatgaaca atatatggtt cccctgataa tctagattgt cattcatgtg cttttttggc
    27541 taaatgatat gcttttaata ttcattcttt ggaattttcg ggtaaatatt tttgtgcttt
    27601 tgtaataatt tcttgtacca aaatatctag attttcagtt gaatctattt gataatctaa
    27661 aaaactatct aattgattta tatccattta taattgtaaa ctaaaatttg ccaacaaaat
    27721 gtgtccaaaa taagtttaat acaaaactta ttgcaatatt tgtataataa aaatactatt
    27781 attttcaaac caaagtttat acaatcgctg gcaggaaaaa taatttataa atgaaaaaat
    27841 agatagtatc ttggagtaga atttttttta ctctacttgt tttgtgtgtt tttatgatat
    27901 ggtgagattg aataatcaaa gtaaaactag tttattatat atattttttt acatatttta
    27961 tttttatatt ttggtttttt agttcaatcg tgttatgggt taaatgtagt tggtttacat
    28021 aaagagtaag agttttttgt aattaaaaaa catctatttt agcctaaaag gggcgatgta
    28081 tgtttatttt tttaaaaaaa atatagaaaa atatattatg aatcttgtta caataaataa
    28141 atttatggat agttttcact tataactgtt gtggcttgtg ttacaaaaat ttattcctaa
    28201 aaaatttata aaatagtaaa tgtttttttt aaaaaaagct agtagcatta atttgcttgc
    28261 aattgtcaat aaaaaaacta tacttttggt gctaattgct ttgttgtata ttttaatatt
    28321 atatttttat taaaagagat atgaacaatc aaaatcttat agatcttgca aaacaaaaat
    28381 tacttgacaa gaggatatcc aatggtttta ccttgttttt aaatgaaaac caattccctc
    28441 gtagacagac tgaaatagca gaaataatag gaagaagttt aattaaacaa ctaatacctt
    28501 ataattctag ctattgtttg actagaaaac aaacccaaca acttgcttta gtgctgtgat
    28561 ttgatttaca aaaagatatg agaatatttt tacaacaaca tgggttaaat gatagtgttg
    28621 atattttaag gtcttctaga aaaggaaaaa ctggttgaca agttatagca gaaggactaa
    28681 taaatcaatt tcatataaat cttgtgttaa ataatagtag gccaggacat aaattaaccg
    28741 tatctcgtat attggatttt atagacggat tgtgatttaa tctgtatgaa gatattaaaa
    28801 ataaagttga acacgaaaaa tctacatata cttctgtttt gatgagtatg tttcctggtg
    28861 gtggtagtgt tttagaaaca tttaaaaaac gctactgaga aagctcggaa tatagttttt
    28921 taagaaaaca gataattttg gaacttatca aagatataaa attcagatga ataactttag
    28981 atatatttat aagcagaagt ggagagatac ttcaatctac tgctgaagga gatttatgac
    29041 tttcaaatat gaatctttat aaaaggctcc attggatatt gggataccca gtatgatttg
    29101 gattgtcttt aaaggtgaaa aaagaatttg cagaaatttg gaaatatcat agttaccctt
    29161 atatgtaatt attgcatttt tagattgatt ttatcctttg aaataactat actatgtata
    29221 gttatttttt aaaaatctaa catttatttc cgattcttat tatcatgcac tacacatcaa
    29281 aagaggttta tcaatcaatc tctcagcaaa caaatgatcc gattgtggaa tggaaaactt
    29341 gtcgtatttc atgagctgag tttccaattt ataaatcaga tttggatttt tatgataaga
    29401 tttctcctgt ttttaacgga gtgagatatc aaattcctac tcctacactt tgtccagaag
    29461 aaagacaaag aagaaggctt atttttagaa atgatagaaa actttataga ggaaggtgcg
    29521 atcttactaa taaacctatt atcactattt ataagccaga gtctggctac aaagtatatt
    29581 ctcaagaagc gcgatggggt gataattgga atgcacttaa ttatggtcag gattatgatt
    29641 tttctaagac ctttaccgat cagtttgcta atttgtatag atctgttcct aaaatatcta
    29701 acttagtaat gctatctgaa aattgtgaat ataatgttat tatgggaaac tcaaaaaact
    29761 gttacatgtg ttccactact tttcagtctg aagcgtgtag ttattgttat cgatgtgtag
    29821 aatcaaatta ctgtgtggat tgtgttaatg tacttagatg tgagggatgt tatgaaacag
    29881 tagatagtac ttcttgtaat aatgtacaat ttggtgaatt atgtaatacc tgttctcatt
    29941 caaaatatct ttttgattgt cagggatgtg ctttttgtat aggatgtaca ggacttagaa
    30001 acaaacaata tcatattttt aataaagctt atagtaaaga agaatatcaa agactatcct
    30061 ctcaattatc agaagataca attattactc gttttaatga gttgaaaaaa acagtaatta
    30121 gagaaggtat gactcaagta aattgtactc aaagttttgg aaacaattta accgactgta
    30181 agaattgctc ttttgatttc aatattgcgg atgctacgaa tagtaaatat tcctatgatt
    30241 ccaatgttct gattaaagac tctatggatg taagcatgat aggagataag gtaaatttta
    30301 tgtatgaatg tgttgcttct ggatatgagg gttatcattc tgctttttct aatctttgtt
    30361 ggactaatga ttctctttat tattgtgatc attgtatgta ttgtaaacat tgttttggtt
    30421 gtgtctgact tcgtaatcaa gaatattgta ttttgaacaa acaatataca aaagaggaat
    30481 atgaaaaact tgtccctcaa attatagaaa aaatgaaaat tgataaagaa tggtgagagt
    30541 tttttgatcc gagtatatct tcttttgcat ataatgagac cgctgctcac atgttcttac
    30601 cactttctca ggaagaggct attgaaaaag gatatattcg actggataaa gaatacccag
    30661 taaacattcc aaatggaatt gagacgattg cagcttatga tctacctctt tcttctgatc
    30721 ctacttctga agaaattctt ggtaaagcaa tactctgtga aaaaacttga aaaccattcc
    30781 gtattatcaa acaggagttg gaattttatc gtaaacataa tcttcctctt cctcgtaaac
    30841 atcccgatgt gagacatgaa gaaagaataa gaattagacc taaaagagaa ctccatttga
    30901 gaaaatgcga tcattgcaat gttcaaatgc tgtccgtgta tgataaagtt tatcaatgaa
    30961 aagtatattg tgaatcgtgc tatcagaaag agatttatgg ataactaagt ttcacacaga
    31021 aatatttgac ttttttttga ttttgtttat aatgattagt gtataaaaga atatttttat
    31081 ttataaggtt tttttgaatg actcatctta taagaatata tcattccttt gataaagata
    31141 aacaaagttt tgaagatttt ttagatacaa cttatgaaac tctaaaagga aaaacattta
    31201 cacttggttt gcactatctt aagggagaga ttttttattc tcttgatatt gatccagtta
    31261 attatccagc ttttgagtcc ttattctatt ctcgtttcaa tgattttcaa attgttgcag
    31321 atgacaaaaa aatatggaat tatgataaac aaaaaactgt cttgggtgaa ctttatctag
    31381 aaaatgaagg ttattatccc tttaagatgg atcatgaatc agatgtgata gcaacaatgt
    31441 ttagaacttt tgaaaatttt gatctcatta atgataaggt tagttttttt gttgagttta
    31501 ctcctgttga tacagcgaac tttctctttt atttaaaaac taaacttaag catcgacgaa
    31561 aaagaaaaaa aatagatctt ttgtttttca aatacctctt tattcatact ccacaaaaaa
    31621 attggaaagc agaaggtaag acttttttta agaaaaaatt tagagaaaat ttgtttgaaa
    31681 taaggacatt tattattgca cagtcagata gtaaagcatc tgcagagggg aaaattcagt
    31741 ctttatttaa taatttttct gtatttaaaa accatctttc aaatcaattt cttttaagaa
    31801 aaatacacca tccttttcct cttatttcac aacttaaaag acaaaaatcc aatctttcaa
    31861 ccacattgct ttctagtgaa gaagtagcta cattcttcca ttttccaaat tccccaaaaa
    31921 atgaaagttc tctttttact gtgaaatcta aaaaacttgc actacctgtg ggtgttcctt
    31981 cttttgatta taccaaggat gaaagagggg aggtttatgc tgttaatttc cctcgtgaag
    32041 caaatattgt gggaatttct gattatagat ctattactgt gcctgttggt atttatgatg
    32101 aagacagact tagacacatg tatgttatat gaaaaacttg aacatgaaag tctaagtttt
    32161 tactcaattt aatgatcaat gatattcagc aaggaaaagg tattggaatt atagatcctc
    32221 atggggattc aatagaagaa gttatgacct atattccgtc acatcgtcag aaagatgtta
    32281 ttatatttga tccaactgat attgattatc cattttgtat gaatccgtta gatgttaatc
    32341 ctgatgaatc taaacaagta cttgcaaagt gatttattga tatttttaaa aagttttttt
    32401 gagctaattg gaactcaaaa ctagaacatg ttttgagaat gatttttctg gcattattag
    32461 ataaaaaatg atctacactt tttgatatta ttagagccct tacggataaa gactttagat
    32521 acgatatgat tgagagtatt caagatgatg ttgtgagaaa tttttggact aatgaattcg
    32581 cgtgacgatc tcagcaattt aatacagaag ccattatgcc aatcttaaat aaagtaggac
    32641 aacttttgtc tattgatatg ttaaaaaata tttttgcatc ttcagaaaat aaactcgatt
    32701 tgagaaagat tatggatgaa caaaaaattc tattggttaa actacctaaa tgacaactac
    32761 aagaagatat tatgggattc ctcggagcaa tgtttgtgac aaagttgttt caaacagcaa
    32821 tgggaagaca atctcttggt aaaaatgaaa gaaaaccatt ttttctttat gtagatgaat
    32881 tccagaattt tacaacagac acttttagtg aaatactttc agaagcaaga aaatattgat
    32941 tatctttaat tgttgctcat caatttatca aacaaattcc atctaacatt tcagatgctt
    33001 tattttgaaa tgttggaaca ctgattagtt ttagagtctc ttccgaggat gcggaatata
    33061 taaaacaaca ctttcaacct tttctttcag gatatgattt agccaatcta aatatgagag
    33121 agttttattg taaacttctt gttcaaggtc aagttaaaga tccatttagc cttaaagctt
    33181 gttggactcc agatgttgat attaatagaa atttggttga tgaactttat cgttggtcta
    33241 gaaaacaata tgcaagatct ctttctgaag caaaacaggt ggtttccgaa caacagcaag
    33301 atgttataaa ggttattgaa gagtttgtag aaccaatagt ataaattttt gcattttgta
    33361 gaaaaataaa aaaattacaa tattgtaatt tttttattac aataaattta gattatttgt
    33421 tgctattttg ttaagaatgt atatctctta gataagaatt ttatcgtaag cataatattc
    33481 ctcttccgag aatacatccc gatcaaaggc atttggaaag acttcagata agaatatagt
    33541 tattttaatc gtctacattc cctaaataaa aaaatgtacc cctagaattg aatctaggag
    33601 tacatttttg ttattgtgtt tttttagttg ttttttagtt tttcaatttc ttcaacaaat
    33661 ttttctacag gatatgctca tggtattaca acaaattttc aagtttctct atctattatc
    33721 acattacctg gagtaccact tattccaaat aagtttcttc attcattagt ttgatcatta
    33781 acttcttgag tatatttacc agaatcaata cattttgtaa gagagtcctt gttaacacca
    33841 agtgttcaag cgatacttaa tattgtatct gtatccaatc atgagtctgt tgcaaacaat
    33901 tgttttataa atttgtgcct aacatttctt tttaattctc caacacattc tatagcttct
    33961 cataatttta tggcttgact gctggacaca aaacttctaa aaattgaatt aacttcactt
    34021 ggatattttt caattacatt gtcaataact cattggacag attgtctcct acagtatcca
    34081 catccaaaat tagaatattc caaaatagta aatctagctg actcttctca attatcaaca
    34141 ccattggctg ttatctcttc taatttttgt attacatctt ccggagataa aatgttttca
    34201 tttaaattat tgttttcggc attcatgttt atttgagata atgcttggtc tatggccatg
    34261 gtttgttgag aaatatattt tgaagaagaa taaagttgtt caactttttt catattattt
    34321 attcaaccaa cctttagagt ttctagtgct cgagcatctt ttttgaataa gtttgctatg
    34381 tttaatatta aaattagtca taacaaaata attattcaaa tgttgtaatt ttttgacatg
    34441 tttgtctaat ttttaagaga gtaaatatta tatcagttta taaagttcat aaagtttgta
    34501 aagttattaa gagctttata acttgcaact ttataaactt ttaactttca acttttaact
    34561 tgtagctaac tattctagct caatatcaac tatgttttct tctacatctt cttccaatgc
    34621 tcattcttct catgctatca tttcgtataa ctcttcttcg ctaagatttc ataacatatc
    34681 catttgagat aatgcttggt ctatggctat ggtttgttga gagatgtact catcagaatt
    34741 ataaagttgt tcaatttttt tcatattatc ggctcaacca acctttagag tttctagtgc
    34801 tcgagcatct tttttgaata atgataatat ctgcaatatt aacattattc atagcaaaat
    34861 aataagaatt aaataattat tttttctttc cattctttaa ttttaaataa taaaatagtt
    34921 tttattgttg tcatgctaag tattgatcta tggcagaagt ttgttgatct ttgtataagt
    34981 tagaattgta aagttgttct acttttttca tattgtcagc tcaaccaact tttaattctt
    35041 ctagctttaa tgcaggatct tttctaaaga aaaagaatag tgcaaaaagc aagtttaaga
    35101 taactaatat acatacgatg atttttgtaa atttttcaca attggtggaa cagcaacaag
    35161 tatgaacagg atttgttttt ttggtttcta ctgttactga aggtttaact actttttttg
    35221 tagaggtctt tgttattgct tttttaacta taggtttttt gatagaaata gcttttgtgg
    35281 cagaggcctt tgctactgtc tttttagcta tagatttttt agtagaaact ttttttttaa
    35341 caggcattat tgaatataaa aaaatataaa aaagttaatg ataacaaatt acttgtatca
    35401 aattaaaact ttaaagcaat agtattttct tgtatttgca catttgaatt tgacaaataa
    35461 aatagacaat atgaaattag tttatttata aaaaaatatg tttgtgttaa aggataatta
    35521 aaaattttaa taaattttga tttttgagat tgaaaattat tttgaaagta gattttttaa
    35581 ggatttattt tcttgttttg tgaacttact taagtatcag cttagaattc cgaaacttat
    35641 aaaaaataaa taattgacca tgctgtcttc aaaaacatgt aagaataggc caattactaa
    35701 aaggacactt cgtcaaatat agaatctatt tcgatgtaaa taaactaact cattactaat
    35761 ctctcatggc tttgtttttt cttttggatc tggttttatg tttttaattc gtttgaatac
    35821 taaaagcatt tgtagtatta cgatacacca aaagataaat cagacagttc agatatctag
    35881 cattagttgc ataaaataat tttctggtag catgcttcca ttgtgatgta ttgctggtcc
    35941 agagcttcca agtcagtatc agagaggatt atctataatt tccttgatat aagaaacctt
    36001 tgcattgaca tgatttatgg tggattcctt ctttagaaaa cttatcccta tgagtcataa
    36061 aataaatact caaaaaattc aaatagcaat ttttttgttt ttcttgatcc gatcttttgc
    36121 aagtaaaatt attattaaag caactcatat tatcgctgat ctagaaagtg tcatagcaat
    36181 tgccagcact cgtaatacaa cataaagaat atctagattt caaattgggt tttgaaaaac
    36241 tttttttatt tttttgattc agcttcatcg tcgtagtaat attagtggga aaaatgctgt
    36301 gagaaaataa gcataattat ttggtccaga aaaaattcat tgtcgtctgg ttgttcattc
    36361 gtatccagtt aaataatata atggtggatt agttcaaaaa taaaaatcat caaattttca
    36421 atatcccaaa ctcataaata attcaggtct acccattttt accgactgtc gtaagaaccc
    36481 gaaaaataca attcacaata aaaatcgctg aaaccaatac atgtctttta gtttgaaatt
    36541 tttgacacat gaaaatcaga taaaacttgc tgtgagaaat atgaataaat aatagaaacc
    36601 atatttaatt caaatcataa tatttcataa agattttcag tgcaaaaatg atatgagtac
    36661 agaaaaaact attaaaacaa tgaatcgtat tcgaatgttt tttcgtttta gcaaatattc
    36721 ttttatttgc tttgtatttg ttaaaagtac aaataaaaaa aaccatattc gtagaatgtc
    36781 tctagaaatt gatattatgt tttgatacca tcatcacaaa atcccatatc ataaaattgt
    36841 aacaattatg tgatataatg ctataagcac aaaaaatgtt ttcacaaacc ttgacatcaa
    36901 atggcttgaa aaaagataaa gtttagatag tatataacat atatttttta aatgaaaaaa
    36961 caatgacaaa tagattaacg gtagttatac ccactagacc aaatattcaa aatattcatt
    37021 gaatattttg atctttggat gtccaaactt tccaaaattt caaagttatt ttgatgatag
    37081 ataaggtcct tacaaaagag gagtttgaag atttgaaaat tcaatcatta aaatgattta
    37141 aaaatatttc aaacaaaatt tactttgttt ccaatataaa tactgatttt atcccacaac
    37201 aatgagtaag ctatgtgaga aattattgaa taaagttagt tgatacggaa tttctaaatt
    37261 tgtttgatga tgatgagttg tttgactctg attaccttca aaaaactttt gatattcgac
    37321 atgaacataa aaattatata caaaaagatt ttgtactaac tcctactttg atgtttagaa
    37381 aaacttgaaa tattcaaagt ttgtgatttg actactataa ttttcgaatt tctagacctc
    37441 atgcttttca attcaaaaaa gatcaaaaaa tagggcaaat acaaatgtat tctggaaatt
    37501 ctctttttgg acccacaaaa atatttcaag aaattttgtt tgatgaaaag ttggattttg
    37561 tgtacgaaga tttagagttt acttatagaa ttcataaagc atgatatcct atttttgtat
    37621 caagtgaatt attattgtac catatggaaa gggataaaaa tattcttgaa catgcctggg
    37681 tttgaagcac atatgcggca tatagaaaag caaaacatag gatgattttt atcaaaaaaa
    37741 attgaacaaa attagaaaaa attaaatttt acttattgtg attccgagca caacctttat
    37801 ggttaagttt gaaagttttg ctttatgcaa gacgagatga aaaatttaaa ataatttggt
    37861 caattattaa atgaacttta gatggtatga aagcttaatc tattaaattt ttgattacat
    37921 cttgcatttt aagtatcttt ttttggtaag gataaacttt agtttttgtg cctacaaaac
    37981 tgcttatcaa aaacaaatgc agtcttgtgc ttattacaag ttctgctttt gctaactttt
    38041 ttataaaatt atcaaaatcg ttttcccaat ctaatacttc aaaactacta ttgtgctttt
    38101 tatgtttttc tttgatttgt ttataatatt gtatgtcatt gtattggctg ttttttcatt
    38161 tagcaactgg tacatacaat attttgtatc aatttgataa aaaatctttt atatcattac
    38221 taatattttc taaaaatctt tctcaatttt tatttacatt tatgataaca tatttttgtt
    38281 ttttatgatt tgcatttttg ataatattcc aatcataagc aaaaaaactt gtatccataa
    38341 aaaattcagt attttcaaat caatattttt ttaatccgta tacactatct agatctctag
    38401 cgtaaatata gtttgttttt tttagtaaaa aagaaaaaag ttttttattg taccattttt
    38461 ttggtacttg tatcccaccc attaaatata tgtttttgtg ttttttgagg caagggaata
    38521 tggataccaa ccaatatcga taattatttg gaagttcttc tgtgagaatt tcacctcctc
    38581 agactataac actatccatt tctttatatt tttttattgt ttttcttcct caatctttta
    38641 tgaattttcg aaaagatcta aatcattttg gtatttctct taaaaattca atttcatcta
    38701 catttataaa ttgtttgaaa aaagatttta gccaagagat attgtaggca gaaatgtaaa
    38761 tctcttttca ttgttcttgt aaaagtttta tagtaccgag gagtattagt tcatctcaca
    38821 tgtttttatg tgatcgattt cctactatca aaactttttt accttcaatt tttagttgtt
    38881 tatttaattc cattttttaa acgaatataa aaaatcgcta caacagtgta tagcgattta
    38941 tatcataaaa gcaaatttta tattttatta gttaggaaac ttttcattca ttattccatc
    39001 gttcatttaa gtattttaca tatgactctt tattctttca taatgttgaa gaaatttttt
    39061 caagtgtgtt tttataaaaa tttgagttta ttaccttagt ttctggattc catatctcat
    39121 atcgttttgc attatcaaac tctatatctc ataataaagt tatatggaaa gggtctgggt
    39181 ctggagactt tgctgttttt catttaaaat tgttttttat aaaatcagtt aactgagctg
    39241 ttttaaacat ttcttcataa ctttggaatt gtatatggtt tcaagcatta tttttcaatc
    39301 catttatggt ttttgttgtt agattaattt ctggatgttt taacatttcc tctaatatat
    39361 aatttctgtt atcatcttta agttttacta atcaattttt catccattct atcgttttgt
    39421 gatttccaaa gttgtttgga tcataatctt ttgtcatttc atatcgagcc tgagcactta
    39481 ccggttcaag tcactcaata gctaattggg tatttacagg agactccgcg gtattcttga
    39541 agtgttcagc catttttggg gaatctaaaa gtgtttttgt gggatcactt caagcttcca
    39601 tttctaatga ttttatggag ttgattcata ataaagataa tcagtcacta agtaaagatt
    39661 catctttttt tattttatcc atagatttta ttaatctttc ttgttctttg tcacttagat
    39721 tggtttgttt aacataattt tcataattat tataatttaa ttttaattta ttatattttt
    39781 cctctataat tcataaatct aataaagtct ttattggaat tcatcatatt gtttccattg
    39841 ttgcaatagg tggttttata tatctttgat attcagcatt attttttacg cccaatcact
    39901 cttcaatttt ttctgggtct ttgccaaaaa ctgtctttgt ccaatcaatt actttaggga
    39961 tattgaataa tgctagaagt catcctcagt ataatgcaac atttgtccat tttcatggag
    40021 ttcatttttt agcaaacaaa ctttgtataa acttaaatcc aagtacaact catcaaacta
    40081 tggcaccgat tttaccaact ttagctcgtg cctctttttt ttctggattt tctcatctat
    40141 acacaagatc tgaaaacata tttcatattt ggtcaaacgc tcaaaatcaa gctttggaac
    40201 aatatctatt gttccttgta atttgagcat ttcgatattc tggagatact gattcttttt
    40261 ttggattgtc aattttttgt tttttagtag ttatttcttt gtaaagtcat ggatttattg
    40321 attgtatttc ttggaaatct tttttatgtt tttcataaaa tttgtcttgt tctggatgtt
    40381 ctatatctcc agaccaaact caaattaaat attcttgaaa tcactctaaa tctttgttga
    40441 gaagctttgc caaagtggca tcttttaaca ttttgttata ttctacatta taagatgctg
    40501 ctatgtcctg gaataatact tcttttttaa catcttttat agaatctaac atagctggaa
    40561 tttctgcagt attttttcat gttgaaacca tttgacgaac tttttctaat tgttctttgt
    40621 gtcacttcat tgtatctaca taatctgcaa aataatccat attggtagta taagtatatc
    40681 tgtttaattc tctttttatg ctttttaacg aggatatatc atcttttatt actttttcga
    40741 atctgtgtct actatgttta ttctctgagg atttgggtgc tcttggtcgt tttttatttg
    40801 cttcaaatcg atttaataaa ttgtcaactt ctgattttaa caattctgga ttatctctta
    40861 tatcgtcaat ttttgttaat tgttctacat ctttatctaa tttttgtatt tgtttgttta
    40921 tcactcattc ttgagttttt tttagtttat tctgggtgtc catttcatta aaaaattcgg
    40981 ttaatagaag atctgactct aggcttactt cttccttttt ttgttgttct ttcaaataat
    41041 cttgaaaaat tgttttatct acataaaatt ttttaataaa ctcgttatta tctgatattt
    41101 gactagagtt tatgtctctt tgttcaaaaa tataattttt tcattgttgt tttattttta
    41161 ctctatcatt tactagatca tcaaaaaatc tgttatcaaa ataatctttt aactgatctt
    41221 tatttatccc tgtttcattg ctattaccag gaagcttttc tatctttatg ttttcaaaac
    41281 cactcatttt gcattttgta gaatagttaa aacttacaat tttttatact ctattataac
    41341 tatatttttt tttttgttaa aaaaatgcca aataattaat acaattggct tggcattttt
    41401 gtaatatagt atatagtatg ttgttttatt ttaatttttt ttcaatttcc ttttttacct
    41461 tatcttttca tggaccactt aaagtttcct tactaattca aaattctcac attatcagag
    41521 ctaataggtt tagtaaaaca tctttattaa ttggcacatt atcgtggtat tttttggata
    41581 atttttttat ataagctatg tattctacca tactgtttcc agatttatcg ttgagtaatt
    41641 ctgtaaaata tatatttaat caaaatttat ttatctcatt taaaaaaaat ggagcgaaaa
    41701 atcaaattac cacgatatta tttatagatt tccttagata gatagtttgt gtcctagtat
    41761 ctacaacctt gaaaaatgta tcgtatatag ctgaaattag ttcttcgttt tttatatttt
    41821 ttaatagaaa tgacaaaaat ttagcaatat tgttgtttgt tgcttttgtt tttttgatct
    41881 catcttgtac ttgttttgct tttgcagaat ctcattgtac gcgttttata tcctgttcag
    41941 tcactttact gatatcttgt ctgacagatt ctcaagcgtc tattcattct catggactga
    42001 atatttcatt taccatttcg gaagtttatg ggataaaggt agtattggtt gctagtcact
    42061 gtagtcaata gtcgttagac attagtcgct agtcattaga cattagctaa cgactattga
    42121 ctaacgacta gcgactataa actatctatc ttttatgttt ttatacttga atttcaatca
    42181 aatttattta tacaaatatc accacaaaaa tttgaggctt attttttttg tataaggaga
    42241 ggtggatgag tggtcgaagt caactcactg ctaacgagtc agccgagaaa ttggctcgcg
    42301 ggttcaaatc ccgccctctc cgccatcaaa attaaaatgg ctttgtagct cagctgcttg
    42361 tcccgcagag catagcgaat tgtgggagtt gaagcggagc gtcccgatca atcgggaagg
    42421 tcgacagttc aagtttgtaa aatcaattaa aaattcatgg ctttgtagct cagctgattg
    42481 aagcggagcg tcccgatcaa tcgggaaggt cggcggttca agttttaccc cgtcgaacgg
    42541 ggctgcccaa agccaaattg tttatctata tgggggttag agcgtcccga tcaatcggga
    42601 aggtcggcgg ttcaagtttt accccgt
//
LOCUS       DAOSID010000002        42213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_1076_length_42213_cov_14.576308, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000002 DAOSID010000000
VERSION     DAOSID010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 42213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42213
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1076_length_42213_cov_14.576308"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PK674_00190"
     CDS             <1..335
                     /locus_tag="PK674_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=25
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOG14996.1"
                     /translation="GENYDMIIFIGGGGAYAQYFGNKEYLRIAKEAKKLGAICITPMI
                     LSASGVFNGKTVTSRDQNGVQKKFIQNNGGFWVDKNVVVSGNIITANGPESAKEFAEK
                     CLEMIRNS"
     gene            553..2712
                     /locus_tag="PK674_00195"
     CDS             553..2712
                     /locus_tag="PK674_00195"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOG14997.1"
                     /translation="MLNKNFRYIALDFETTGLDPKKDEAIQIGLAEIDVNGKIIKEFK
                     SFLKPQKDIKELKDFVAYITGISLDDLQEAPSIFDLQKEIFDFLGENVILIGHNIQFD
                     IDFLKKYFQNIKYYDQIDTMYLAQNFVHFAPSYALDVLVEYLFPKKEFKKNFQNIHQI
                     PDFDSQSTHDALFDSKNSLCLFVYIVQDILFLIKTYPILSNFIQKNLGLYHKILNYKV
                     DRASKKNWKLELPKLEKQFPGEISLKSKDKINLRELKHKGKYFIGNVDIDKIIATIVG
                     SNKTIILSFASVAKLNIVKNILNDMGMKNIGFVRGNLIINKTKFNTFLNKESFSDNEL
                     LFIFKYMSHVRHKMSILDLNTKFDFKINYYIQDDKKNKKYPVILTTHLGLFTILNEKK
                     HIYYKYDICFFDPEMWYRSYNSFLSNNYDLYSSLNFLETLYYKYTLNNQESTKELLEN
                     FARFFEVFMGVLFAETKKHFVNIQDNYITLNPIIDHINFHETNALIKQFDKHKTLLEA
                     DLDSEDFKKLWGGIDKMFNILSGLVQINKIMYGQSDFYFTYGEATNFTNRDEFADIFA
                     SHTLFLSNFDKSCNKIMNSNDIEYTKYNLNFKKIGDIDNVISFLNKELSTNSDKTFFV
                     ISTIKLESKELFEKIYEAGLDQKALLLVENITGSLGKNIFKAKNNGPKIIVGGYNFFI
                     RLLSNKINIDICVDFNIKGKLSEYLLNDMQRYAKYKK"
     gene            2693..3661
                     /locus_tag="PK674_00200"
     CDS             2693..3661
                     /locus_tag="PK674_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14998.1"
                     /translation="MLNIKNNNLFILLKFIGAVIIFYLGIKFSVCFVGEKTSIVDEQL
                     YNTGTSMDNLDESRKDLLEKHEIAETEPTETIDDYKELKEEQNEYLLKFQTICSSNAG
                     LCAKIKFQGEFDAQDKYMYLASSLYVLKHIENNQQFGKAVKQQLKEIKINNEIASRRG
                     FAGWDTVTINLGMVKSYVEFLELITHEMGHIVDLGMIRGFSKQKDGNYTEFGNEVFEI
                     DDPSIMFYKLSRQSEKIRKADARKDDFCSSYGMTDPFEDFAECHNLYLNHNAVFVYRA
                     KNNEIMKQKYNFIANLYGGKYLFDSTKDLDRAKTNGSWRPWDTTKM"
     gene            3804..5501
                     /locus_tag="PK674_00205"
     CDS             3804..5501
                     /locus_tag="PK674_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG14999.1"
                     /translation="MKKSKKTIIYLIIIISVFVFQNVFFNIKLFADDNVKVLEAIAES
                     CIVHGIGYSFDKDKGCVCDNPAKDDKCENGFVKNKYGCCIGSCGVCSSNSVEFQKYIN
                     FQVELYGLLLEELNQDKENKQSGGFRQGGLFSSRVLSIPVNIRKSRTKIFKKVGQEYI
                     DTKSTIVVGSTIITSIVTESRKDMLGGLMILYKSKALVRERNTIQSLDLIVYDLMRDL
                     NTTGLWNKEISNDTKIKLEQLNKKYIKTEQNQKGMFEKFYLQGSIKYKDLINVSKKIN
                     SLMKNFILTGKEGDSFMKSFEKEFSKGNIVVQFSEHRKNNLINDYVCARRISACKEDF
                     SHLINVNGLKNSFSNSMSIIKQANKNLAEIFTDKEKHKDKESNPYGLTDKQIELLRTI
                     YGINTSKITQQQGVTLGYVFKKTAQVYNQIKNQVNLKISDYATKKQEQENKKNIKNTK
                     NKEQDEKYINKLSKEEQKKIEKALKEQTEKIEKGETTIAFTNIIETILNERDENKEII
                     LFYDTNSTNRYFTETIGLIQDIIKNDIGNKDTQGLVKYLGEACTYQCANKGTTNCFK"
     gene            5514..6668
                     /locus_tag="PK674_00210"
     CDS             5514..6668
                     /locus_tag="PK674_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15000.1"
                     /translation="MKKEKTIIKTILIILLANLGVYFSFASAGNLSCNSMGLLLSADL
                     TKLSELGSVKLISQNFCEHVHNSKCEDNIDAHDSDYFDANQSIFLTLLCDNVGKGQNF
                     TFINRTENDSILKKRAFSDFNIYNYNMEDINYCDSKTNMNGCDFPKHLPKMFNEIIND
                     YFNIGQARIYGIKGFQEKINPIISANEFSDENFLIKICDPSNKQYYTKSCKYLSNYMK
                     KANQLLIKTKVVDVKKLTEQNKNFDCDNKFSENILYCGLLGSDNNLSDNFLNLIYNEY
                     LWYRLFMEYYSTNLSTNPDLSNLISNSNAEKLKDNKEKIIGLQQNNTKIKKALSDSLR
                     TLSEIAQTFPLHIGFTMYQENANIFMKELSKIYPPIRTLADKLKNVQKTD"
     gene            6649..8772
                     /locus_tag="PK674_00215"
     CDS             6649..8772
                     /locus_tag="PK674_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15001.1"
                     /translation="MFKKQTKNYIHKIIKSKNIIIFVFLMVFFIDFSFSFEISLKDSI
                     EKIQNTIKRNSEISIGGKAIIDDIYKTSLNKIQIDNITPLLIATDKTAEKINKDYTCT
                     MNNQEIIEILYFTNASFKHELKNNLEDFKNPTKDRIGINCNKLNTCIYKDNLAPGQVF
                     KNTVSSNKSCQNIVENIFLTFYIDSYYNGNIGEQIRGTDTFWNNDLSDSNYDLLNDIF
                     KLGTILFQDIKSPAKILFYSMPKINYNNIYQEPKLNMKIDWFSPYNIFYGDEGGNNTN
                     SGNTGDTSQINSGDNLGNNLGNNLPIINEGMQNGGISGEIESSIKNFINSVNINLNNP
                     SSSFVGNQCVSGFESEKYMISFSTADGENTQTTPQEYLESVIEEIEDLNCNNDSICQD
                     RESDSCPDCQSSSYDPEDPSSFEEIQAILENIIEQGEEIPEDDPVLGCVQKCDLMPCG
                     ATECPKLVCYAKCFCQIYSFDTDDLDLTSDNTNITGLNKPIRSIGLSSKFAIKFCIIP
                     AIGGEVQTKTIYSITSIFSELNNILENLKNSGNMGINIKPKEFLEHTKKEYNFAKQIS
                     FNINSAKEPVSSNQSKDGMSKEQINLNTDLMEQILGFSKEYNTDKEQNKYILIDDPCE
                     HKVIVQASNRDEKEALLESCRQESNMNPTVNNANLNPAMINQNIMLINIEFDDFMEKN
                     RDFWYETKLMFDALKNTAQSLENKK"
     gene            8911..10413
                     /locus_tag="PK674_00220"
     CDS             8911..10413
                     /locus_tag="PK674_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15002.1"
                     /translation="MNDNYFSEKITVEPNHKRQKKSVSISTLIISIITTIVCVFIISS
                     KRENIISLFGFDSQIQEQNNDTQTNIGDEISCTGKMYDDGDILNYTHSLESLEFGKLA
                     LKSSKINLNNYKNEVYIEGIVEKIFQGTPVVSVSTIYSLDIEELNENEDENEEKLLEM
                     DYESKFLPNIGIYLDNKFFQKYSLLNEGEAGILKIKKSETNEIININYFICSNSDNNK
                     NCDRFNNMFSTSSTEKFVDTYNINYYKQAESQSRFFSNDSLFGYFINNTDDFLTKDLI
                     KHIQVINKKFIEKNILNKVDLLCQDSEKGIKKIEESSIVLKDKDLLVNLKGSNEEESF
                     ECKLKIDPTLKNMAALLELKSLGKLENKNTNEQTDGNKKEVTANYNRDPNIPQFPINL
                     EKSLKFTSRKGFSYTFPSSNIAYAAQNTQEDFGQIGVNCYSVMNVVQYSEKELVTQKG
                     EVKIYECNIKGDFEESEKLIHKKEGEKDFIIEIVNPARIAFANNITISIE"
     gene            complement(10430..11302)
                     /locus_tag="PK674_00225"
     CDS             complement(10430..11302)
                     /locus_tag="PK674_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15003.1"
                     /translation="MILSLLRESKDYIKNNNKFIKLVFYISFGKLLYTLVLISYNINN
                     LLIYRFDKGLPRMSTLKYFLEEIISNHLVWLVILIAFIILIGYVFLYPTGMSAIIHFL
                     KDKKNSIGKAIGKGVTDFFVMFELNALAFSFGFYTYAITVLRLFTLGILDNTIAIILV
                     SVWGLAALFSSIFWQYAKFLIVIENMGVFEAIKKSISITITNIGVTIRGRVMQLVTYI
                     LFYFKAFIIASAPMLLVYFLINAGLADRGFKRVIRLIGIITVILIIYMISIIQAFFRK
                     FWFKMYNKVVKIEE"
     gene            11477..12703
                     /gene="yidD"
                     /locus_tag="PK674_00230"
     CDS             11477..12703
                     /gene="yidD"
                     /locus_tag="PK674_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOG15004.1"
                     /translation="MIVLKVIDKILAEIIVFLIKIYQFTISPDKGLPSLRLKGKICSH
                     YPHCSQYSINCLKRYGFIKGIPKMTERVVSCTPSKEKIYDPEYYKIVFFSGAPIGVPF
                     LHELQKDKRFEIVGVVTMPDAPSGRGMEAKPNIIKTEAEKIIANHKENTANEFIKTPN
                     SLRLDPKKHSEEAHKFKLRLEAKNPDYLVVIAYGKIIPQYILDIAQIAPINVHGSLLP
                     KYRGASPLQSVFLNQERQTGITIMKMDANMDTGNMIDMLKFDIEFNWTVKDLIEKIMQ
                     KGPQFLNNTLQSFGKKQLGEVKQDENKATYCQKIEKEDGEINPYKDSIQEIYNKYRGY
                     YIRPKIFFQNLSTQKRVIIENLKLDQKLFESEKNKPLFIENELNKCILDISLKPEGKK
                     LMDRQSFKSGYLKSSK"
     gene            12843..13721
                     /locus_tag="PK674_00235"
     CDS             12843..13721
                     /locus_tag="PK674_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15005.1"
                     /translation="MKKRLLFLTLVLFIYGCSTPIQNTTTQDIVKQDTRLNEPNYSIK
                     TESMVKVEPEFIDKSQETPSFEEIDDIVSVCHNNTANCLIDCDDWNITKKSYIIPYKN
                     GYIGFDYGGNGGCRGYFLTYKSLDNPCDETISNTDEIFIRNVSYPNDLERLDSPEYPD
                     NKLYYAQGGWSGIPIEEIAKHLNCKPGKPFEQDDSCKKEVDRFMYNLIIGNEENEYFT
                     QWMEMFKKNLDNNIFTTRSFRSERWKICNKRFSFESLLQENGNDNRTLSNEVRKKYNK
                     IQNERIHKCLIEYQRK"
     gene            13747..14823
                     /locus_tag="PK674_00240"
     CDS             13747..14823
                     /locus_tag="PK674_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOG15006.1"
                     /translation="MSEFKNPLSRSISKSTVLHFCKKKYYFSTYSNYLNEVDKGLWIQ
                     ALLAKNLKSVYMRFGEKMHDLISDYLNLVKTGDDNLDKIKKTKEDLINQMDQEYSVSK
                     NRDYLKYDPNVKFGLTEHYYGYNIDNIYQNGKTKLLEDFDKFIKSEINKEVKEYFEDK
                     SNILFIEAKEKDFESMKIQIENIPELMGINVYAQPDFGIITTNKDYIIYDRKYGKVPN
                     KDKSLVSNQLKVYAYKILQKIGIENVDNFNIKGFEVYLKNLEKFGGKITKQDLEDIEK
                     TIISDTTIQKQFLINQDVEKNQPLPSENFPRTNDLQKCSNCTFYKVCEELKKFEKTHK
                     IEEINNIDNSVNNDDEYNEDGFPF"
     gene            complement(15128..15200)
                     /locus_tag="PK674_00245"
     tRNA            complement(15128..15200)
                     /locus_tag="PK674_00245"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15165..15167),aa:Lys,seq:ttt)
     gene            complement(15241..15429)
                     /gene="rpmB"
                     /locus_tag="PK674_00250"
     CDS             complement(15241..15429)
                     /gene="rpmB"
                     /locus_tag="PK674_00250"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L28"
                     /protein_id="HOG15007.1"
                     /translation="MARICDFTGKGTKSGNKRSHSMIASKRTFKPNLIYKWVVLDDGT
                     KIRVKVDSKLYKKMRGFI"
     gene            complement(15460..16146)
                     /locus_tag="PK674_00255"
     CDS             complement(15460..16146)
                     /locus_tag="PK674_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L25"
                     /protein_id="HOG15008.1"
                     /translation="MKLNVEFRNIVGKNVKKLRREDKVPAVVYSKHLEKPVSILCDRN
                     EFVKLYRQTGYSTPITLIGEGIDELVLIQDIQLDPVRDQVVHIDFLGIKKGVKVSAEV
                     AIVMTGEDLSPIVKSGDASFEVLKDYLLVNALPKDLVKEITVDVSNVTDLDTVIFVKD
                     LVLPEGIVSEEDGELPVITVVDLKKSAAEEEAKEAAAEAATEATEQVSAEGTEDSPSE
                     EETSSEETSV"
     gene            complement(16187..16840)
                     /locus_tag="PK674_00260"
     CDS             complement(16187..16840)
                     /locus_tag="PK674_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L19"
                     /protein_id="HOG15009.1"
                     /translation="MNYERIKNIQKKHSKFILPIKSGMFLEIHENVGEGDTKRIWKFR
                     GLVIRVKKPNHFDGTFTIRGEVARMTIEKIYPLSFPNFEKVILLDNYKIRRSKLYYIR
                     DKVGKDARFESLVKTETKGIDLLDGLRQVKEDTKVEKNNEQKVVEEKTKNEEDTVKVF
                     VENKEKVEESFNDSVEEVIEEKVEEIVEKKIGDNAQEKVENQEVNEADVESEELGEE"
     gene            complement(16887..17009)
                     /locus_tag="PK674_00265"
     CDS             complement(16887..17009)
                     /locus_tag="PK674_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15010.1"
                     /translation="MGKKKLNKALSRVEKVVIMDTRKMLEQQKKWKAKVVNSKK"
     gene            complement(17027..17296)
                     /locus_tag="PK674_00270"
     CDS             complement(17027..17296)
                     /locus_tag="PK674_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15011.1"
                     /translation="MGDTSGRKIKRFLVGLNWKKKKSRQKYKKAIERYILVLKKESIG
                     GKTKRTKDTKQRALARLKKYGITMNQVKEALDRKGLNDLSSKIVA"
     gene            complement(17342..17812)
                     /gene="rpsG"
                     /locus_tag="PK674_00275"
     CDS             complement(17342..17812)
                     /gene="rpsG"
                     /locus_tag="PK674_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="30S ribosomal protein S7"
                     /protein_id="HOG15012.1"
                     /translation="MVAKLNKKIKVVLPSSNEKLDKIINYLMKNGKKSVAKKIYNDML
                     KDIKSSGHMNPQIVVETAIENASPLMMVKSKRLGGSVYQVPVEVKQNKRLFYAMRWIL
                     DAVRSKSGKPMYKKLSEEMLAAYSNQGAAVKKKEETHKMAEANKAFAYMAKYVN"
     gene            complement(17928..18344)
                     /gene="rpsL"
                     /locus_tag="PK674_00280"
     CDS             complement(17928..18344)
                     /gene="rpsL"
                     /locus_tag="PK674_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="30S ribosomal protein S12"
                     /protein_id="HOG15013.1"
                     /translation="MPTINQLIKNGRKAKKKKSKSPVLQFSINKLKNNKKVEMPSPFK
                     RGVCLKVGVMGPKKPNSAQRKFAKVRLSNGAEVLAYIPGQSHTLQEHSVVLVKGGRVK
                     DLPGVKYHIMRGKYDANPVPERKQGRSLYGVKRSKS"
     gene            complement(18590..>21762)
                     /locus_tag="PK674_00285"
     CDS             complement(18590..>21762)
                     /locus_tag="PK674_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15014.1"
                     /translation="SGFNLQPGQSAIVYLTGQVRTEPSMFGGMVQTNWAFTSGAMASA
                     PFLLKPTSPGELGITKTLLNTGQFSSGDYVGFKIQLTNIGSGVFTNAYIRDLLPLSLK
                     LISHTIQGISSPYKSGQWLSGGNWVFEYSGFNLQPGQSAIVYLTGQVKGDASPYHTIN
                     WAFTSGAYDTAYFALKPQPAIQKYQRVGNIPFTTGNINIKAGDLITYKIDFANLGVGN
                     AMGVRIVDRMPLCISYLSSRIMGVNKAKFAQTKDSSGRYIIEYSGFNLSPGQAGYVII
                     TGQLVDSVACSAYTHFVNNSYIYFYNPMNVLVSSTTAHRTQKSIVVLTKDSDPDVHLL
                     GDDKLFEITVKNQGPNKISEIVLQDIWPNPDCISYVAWTGTAGLVKDSTSLVWSYPSL
                     DSGETLTLYISGHISDDLSCINPEYINKVHLKYTELGKNYTDQADYYFSVVGEEKALC
                     DRITTGGIIIINDNYQGSRRFTCYSQNGVLADEIVLNCGNGVVKTGYNASSFTESCNY
                     VYDPNAGVTGNTYNVRCYVDNHTRNECKTAVLLDPGYVGVCGNGTLDIGEECDLWTNV
                     NLISNRIKIGNYLDLDHNYLAGIYGNGNYYCKECRIRKYSTNEFVYQPPQCLGVNGSI
                     SLMENELMPFWWRLWKRQEQFVTNYNCSNIDDDEIITYIDKNSMKCTFSVYDGNKHKQ
                     INDNPLHTFITDCYEKNNSVIFNYFEKAYKIDFTKVAGTYVFNTIVMFDGAVDTYGEY
                     KLVLEKVDYNYCDPATKNWRAGRTYKGICEVDFALTRPYIMQISTFGISPVATSADFL
                     KDFYDMKGEKLIKSTDIEETMSVDSATYGFDSEVVNQMNSFKNKYKSLAIAVDGNFKV
                     RDNVRINDIFGNSTVKKVPNQMIFFVEGDGQLVLKKMTEYFPSVPFTVYIDGMDVLVE
                     GSITTNGMIITTDKISFEDDGDKDYCEEGGQVVNGIFIAQGGFDSRSRTRNTSKNQER
                     CPRGNLHVKGVLIGDDIDNLVNNRRSHLNDWFTVKSSSPSAIKRERKREIFEGAALLI
                     EYNPTLWTQLPPGADSFTEILDVYRK"
     assembly_gap    21763..21862
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21863..23660)
                     /locus_tag="PK674_00290"
     CDS             complement(<21863..23660)
                     /locus_tag="PK674_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15015.1"
                     /translation="MKKFIAIVLFVLFIIGFAKAGNISTNNMSSIYNSCNSNESKDVY
                     YYLDNGVNSLTRNPYDVFGNTLDSLISLNTFVELWRNGNVLTNCSDWNRVSNATVAND
                     PNGVLAVQNYDGCTFNKPNYLKYSNPNKIDAQIHFNIKYHKVFAGAPHSTKNKFFYKD
                     YGQNDWTCYPAGNTVSHYDSCPKVTVKYGVLNSHIGECLNYRIFWCGDGILNRPGGST
                     SYTNGTYVEECDPNDPTHAGRGNAGCSNTCKAVTQEAPTCSLSISSLQNSTYKVSWTV
                     NGSFNPTQINIQPLGFSPNYKSVHTNVGSFNTVAPSGYYGPFSATMSVANTAGSNTCI
                     IVYNIPQPELKVCGDGKVDRPNDNGVYEQCDNGPNFGNGCNNQCQLVTPNCNLTVNPV
                     LGILGYPTAFTMTTNPWAITDFLNLGDTLAGGTPNIIYPTGGYVEYVYPKIGNYTVVY
                     GAKNNYPGAVATGVTRPIVLCTGEVQIMSGCTITSTPHIIKLGEIAKISWQIWPDFNI
                     PAFMVVNPALLGPWPHNIYYNTGEVFVGPNISHTGEYTFSINSHTIYGQAFSCTGVLK
                     VLPSVSPGELGITKTLLNTGQFSSGDYVGFKIQ"
     gene            complement(23746..29979)
                     /locus_tag="PK674_00295"
     CDS             complement(23746..29979)
                     /locus_tag="PK674_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15016.1"
                     /translation="MANQKDYVRDIEKKDNLASNLKSIEGIDKFDFKHFDAEVEKSFQ
                     DFLEDKKNQFTTATTKEVLEPVFNGWLDKEFGTLDSFKEKEIAKQIIVPTTFELQNFK
                     TLVELEIFQTQVINAKMETEKQVNLDLIGVGDLTLKLYRGLDQRKNEVIEELNKNFKD
                     RDTVIGFIKEGNVESIRRLQCMLVGLNYEKGKLQTNDFMKNPGTDGLFGTETFDALKN
                     YINNNPVSAGAGDQNSSVGNATTTTASFGSADIVGTSIGKTPNVGTGNSGVNNIDATK
                     SGLDTGVEIDKKDMVFECKMDEKVDLASVPNGTTFEVIPPYSFDDAKNIPVTLLDGKL
                     QYEGKFTGQDNFTIVVKDGGGKEIYRKNMTVNVEEDEIWKVDINKYESLLAKAEKEVK
                     DIEKNGNKKEDIEKRIREIKTEYKDNLKKIEDLKEKNGEKEEVQKLSDRNNEIEAITK
                     ALVLKEAEIHMKEYLNNSWEILEIKLNENCDYYFGGEYISQKEFMKRLGSERKKIDLS
                     RTRFVNFDVELDNYDSIDKYLIDEKSDKRNFTIKDLILTDDKNFGKGIKVAKNQEIQY
                     KAERYFGSGSEKIKNVQREKRALKRILGIKGLFGRGGTHDDLSISTLDYNTNNPALLE
                     RRNLRKLKKILLNTYKNKDVSIQNMFYDVSRDYDLAIPPRPSQDKDKNKQELFGLYKM
                     YREDQNCGDYYKKVMGLLENYFLRGIDGFHVIDEENNKVLFKNLYEREAIGRADGLID
                     PDKRRLAVNHLFKKSTRKGSVELMNASFADSKELKELKRMVNKIDLSTYLTDPNASKK
                     HEKFTKLYDKYKKSKQGDVKDGNGNFVVMGEKDTSDSKECYYNAVFDFLAGVVEGRQD
                     IVSAINSTKIIDNLVDIDYDEDKEKHRYENETEKKFVMLLGDYNRDGRVDFGDRGYVM
                     GLTVKNVYKSVNVDRKFSSDKDKIKAPWENLMDFCEILMEQNGDLALLSDVKKLKEEG
                     GEDGGLKKILGEFENNPGLLGYFQTTLANSPMPIDYIFRYGKTAQEEYIDYVLPQVEN
                     GTFKIEGLDNAFADQYKKLLEQGLVDDPEIRAVLKPIFYAGVLQNGGISTSVGTGLTY
                     DAGKAGQFTMSLGVGNVAKDKDPVLGIVFSYNNSAKIGKNTRFNFGTGIGATNKSLFL
                     PMAYGTIGITSRLNPGVDLNSLKAKSAHYFSTGGNLTWIPEVGFGGGGYLGRSRNKME
                     LTDEQSVEIKNTLTGTNGVLYKSLAGVDLTKDKENIISGIKQNLAKQFYNKSLNDLKD
                     NEHKLISKVAENLYKGISYYGAGLSLKENDPKLTAIVHEVAEAYAIQWKNDTKLDATK
                     WHLSGAGISIQILARYIPVISLVEFSKYKNLYSKETKQSHANYYEKLVTGRGMNEMEG
                     ENFSLDGYITENGVNYLNAKLRIAHPDVLTPDLDIKLEPMTENADGRAVNVLYIPLDL
                     IKYANINVDSKVVNYTKIVDNKYLLVPANAKIGLMDYSRLNSARFHLFIGDNKAKLDD
                     IQVGYDMEDYQGDPTKYEGNVEAKIIVEKEYVNNQIIKLKNDVREQGYVEYPLPIKEC
                     FEVENGKAIFELEEGIGKQCVNVGGNNSLITIEGDSLIMPQTGTLTMIRQSNDKYYFY
                     YKSNPAEDLVLDYQLGAELTVGTPDGNATSIEMIDGMSWKRETKSFANIDIFLESNEI
                     DRLFDGIEKGLSRMDNNVPSIYGQFMAESFTTLEKTVIEEENYEKAFGSLQEILKNKK
                     NNFSDPIYDNLRTKIEGTVTSEEKIMIVDGFKSMFAYHTMLSEGGVKTHNGQQLTYFF
                     TKRGTHYKELYGYNKNIRFPLENTYRDSLFNDFRSQKTLERRKEKNLFGMTAFYHQNN
                     KEGRGYSMTQMGATNVLGIKNSPNSGIKRIEDKDLSATQEWFWGNLDKTIPHKEILKK
                     TLQKQINIDGLELTDDNLKNLLLGKSIDILDQKVRIKLDLEYVFYLLGECANESIGIN
                     LEGIEVSIKIEDGKLESGGVDGKTDLENINENGHRRVVVKGNKHYGSNLNTSGAIANT
                     EDAYATKQTFGVVTSYTKLGEPGDDPWSDPEDPVEPGDDPWSDPEDPVEPGDDPWSDP
                     NSP"
     gene            complement(30149..30901)
                     /locus_tag="PK674_00300"
     CDS             complement(30149..30901)
                     /locus_tag="PK674_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15017.1"
                     /translation="MSIEVLEKKDKNIEQYIDPEHKVNIIKQGKGTVGEFLSNFGIFE
                     ERHEEVKEEKRFEEIKERKKSVEAREEKELEEGKGEKKLEDKNEREKKEQKDGGFFKK
                     LFSRNKKNDEKDENKDGEQKESSFEKENLTKDKKEKPGFFARLRKIIKNVREIAKDGK
                     TEGKLRGKELKKSESNEGTLESKNPNQKLLDGFANGHSVKSVEYNKTIRPIELLKNAK
                     EINEESPDTGKNDVRIFGQKENPDEKFQEKKE"
     gene            31075..31147
                     /locus_tag="PK674_00305"
     tRNA            31075..31147
                     /locus_tag="PK674_00305"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31108..31110,aa:Thr,seq:ggt)
     gene            31305..32507
                     /locus_tag="PK674_00310"
     CDS             31305..32507
                     /locus_tag="PK674_00310"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="aminopeptidase"
                     /protein_id="HOG15018.1"
                     /translation="MFSPSIEILNKYADVLVNFALRNGKGIKKGDVVFVYIPESAKAF
                     YIPLQETILKAGGHPIMKYMAEGVAKNFYELASNQQLQFLPDAYKKGEIESKTHHIDI
                     LAEMDKHELKGIEPKKIMMRIKANYPYKELLNKKEREKNFSWTVGLFGTPAMAKEVGL
                     SLKAYRDQIIQACFLDKKNPVKKWKEINSWMIKEKDKLDKLQIEYLHIKGEDVDLNIK
                     IGKDRRWKAGNGINIPSFEIFTSPDKRETNGRIRFNQPLYRYGSLIKGIEIEFKDGKI
                     VKASAKENDKLFQDMIKIPGMEYVGEFSLTDARTSRITKFMGETLYDENVGGKYGNTH
                     IALGKAFDECYNGDISKLDNPKFKESIGLNTSAEHVDIISTTNRTVTATLQDGSKKVI
                     YKDGQFTI"
     gene            32647..32982
                     /locus_tag="PK674_00315"
     CDS             32647..32982
                     /locus_tag="PK674_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15019.1"
                     /translation="MASRTNKRGIAAKKAQQQKQAQRLKGLKRFEYPKRKLELEEAKE
                     LGVVGVFKNPKGSEYNRFIRGRKICRETKLRVDPDGYRYIRTPSGGSEMVQVPISGRL
                     RTRMRKNMN"
     gene            33254..33325
                     /locus_tag="PK674_00320"
     tRNA            33254..33325
                     /locus_tag="PK674_00320"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33286..33288,aa:Phe,seq:gaa)
     gene            33388..33464
                     /locus_tag="PK674_00325"
     tRNA            33388..33464
                     /locus_tag="PK674_00325"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33422..33424,aa:Ile,seq:gat)
     assembly_gap    33573..33672
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            33798..33871
                     /locus_tag="PK674_00330"
     tRNA            33798..33871
                     /locus_tag="PK674_00330"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33832..33834,aa:Pro,seq:tgg)
     gene            34143..35315
                     /gene="tuf"
                     /locus_tag="PK674_00335"
     CDS             34143..35315
                     /gene="tuf"
                     /locus_tag="PK674_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="elongation factor Tu"
                     /protein_id="HOG15020.1"
                     /translation="MADKVHVNVGTIGHVDHGKTTLTAAITAVSAAKGFAKAQNYAEI
                     DNAPEEKARGITINTRHVEYETETRHYAHIDCPGHADYVKNMIVGAAQMDGAILVVAG
                     TDGPMPQTREHVLLAKQVNVPKIVVFVNKADMVDDPDMLDLVEEEIRDILTQNGFDGA
                     NTPIIRGSALKATENPSDPVAAKPIEDLLAALDSYIPVPVREVDKDFLMPVEDVFSIK
                     GRGTVATGRIERGSVKVNDEVELLGLGKEPKITVVTGVEMFHKAFDKADAGMNVGLLL
                     RGIDKEEIERGQVLCKKGTVKAHNKFEAEVYVLKKEEGGRHTPFMSGYRPQFFLRTTD
                     VTGSINLKSGTEMIMPGDNAKIDVELIYPVAMDEGLRFAIREGGRTVGAGVITKIN"
     gene            35341..35673
                     /gene="rpsJ"
                     /locus_tag="PK674_00340"
     CDS             35341..35673
                     /gene="rpsJ"
                     /locus_tag="PK674_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="30S ribosomal protein S10"
                     /protein_id="HOG15021.1"
                     /translation="MAKKEKKELKPKLRIKLKSYDVKMLESSVGKVVGLLVRSGAEIK
                     GPIPLPKKRKLYTVLRSNFKFKDSREQFERMTYSRMLDVTEMGPKTMENLQNLSIPVG
                     VLVDIKVF"
     gene            35741..36343
                     /gene="rplC"
                     /locus_tag="PK674_00345"
     CDS             35741..36343
                     /gene="rplC"
                     /locus_tag="PK674_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L3"
                     /protein_id="HOG15022.1"
                     /translation="MNRAGLIVSKKEMTRVWKNGKMIPVTILKVLPQELLRYKTVEKD
                     GYSAAIVGVNKKETNKEKGQKVKYSITVEFNNIDDNFLETYKSGSILDINLLEGVKEV
                     NLVGTSKGKGFQGAMKRFHLRGGPKTRGSKFHRQVGALGNRKPRRVQKGRPHAGHMGY
                     EKVTIKSVEIIDRIIENNEQLLILKGSVPGSYNSFIQIFA"
     gene            36379..37008
                     /gene="rplD"
                     /locus_tag="PK674_00350"
     CDS             36379..37008
                     /gene="rplD"
                     /locus_tag="PK674_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L4"
                     /protein_id="HOG15023.1"
                     /translation="MGYTIDIYDKTGKKISTLKLNNEIFSEDKINYNLIHEYYLLQTS
                     NARNPIAHTKTRGEVAGSGKKLYRQKGTGYARVGDRRSPIRIGGGVVFGPRNERNYTK
                     SMNKKSRQLALSGILTLKAKDKNIIGLKDFEMKEPKTKEALNILTNIGLNKEKTLFVL
                     NEKNINLIKSFRNLNNVKYLLADYLNPKDLLEYNKILFLESALNKINTK"
     gene            37061..37402
                     /locus_tag="PK674_00355"
     CDS             37061..37402
                     /locus_tag="PK674_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:1990904 - ribonucleoprotein complex
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L23"
                     /protein_id="HOG15024.1"
                     /translation="MTYREKLQRRAKKNMETKALKKLSPYDIILGPNVTEKTYKLQET
                     NNQYFFKVHKSANKNDVKQAIKYIYNVTPIGINIINVAFKGRNQRKLVRRSHKKAIIT
                     LNKKDKIELGL"
     gene            37456..38304
                     /gene="rplB"
                     /locus_tag="PK674_00360"
     CDS             37456..38304
                     /gene="rplB"
                     /locus_tag="PK674_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L2"
                     /protein_id="HOG15025.1"
                     /translation="MAIKTYKPYTPSRRFMIGYDFSDLTTKKPEKSLTKFLGKTGGRN
                     NKGRITTRARGGGHKRLYRLIDFRAYDKIDIPAKVASIEYDPYRTCRIALLNYVDGEK
                     RYVLARKGISVGETVMSGENAEIKAGNRKRLKDIPEGVNIFNLEITPQSKGKLVKSAG
                     TYATISGKDDLQGLVFIKLPSGEVRKFNENCRATIGEVSNEQHKNIVIGKAGRQRRLG
                     KKPKVLGVNMNPVDHPHGGGEAHADIGLKKGPKSFTGKLVAPGIKTRKGQKWSDKFIV
                     SNRKKK"
     gene            38347..38604
                     /gene="rpsS"
                     /locus_tag="PK674_00365"
     CDS             38347..38604
                     /gene="rpsS"
                     /locus_tag="PK674_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="30S ribosomal protein S19"
                     /protein_id="HOG15026.1"
                     /translation="MARSLKKGFYVNEKLLIKIKKMSTTGDKKVLKVWDRACHIIPDM
                     IGLTFAVHNGKQFVSVYITEDMIGHRLGEFAPTRTFRGHPF"
     gene            38645..38986
                     /gene="rplV"
                     /locus_tag="PK674_00370"
     CDS             38645..38986
                     /gene="rplV"
                     /locus_tag="PK674_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L22"
                     /protein_id="HOG15027.1"
                     /translation="MSNENMTATLKYAIVSDKKMLLVSKLVKGKKVQEALNILGYIPK
                     KSAKILAKVIQSASSNAVVNGKKKLPDLFIEAIEIGKGPKIKRIRFTSRSRVSHYEKS
                     RCYVKVVLNTK"
     gene            38995..39660
                     /gene="rpsC"
                     /locus_tag="PK674_00375"
     CDS             38995..39660
                     /gene="rpsC"
                     /locus_tag="PK674_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="30S ribosomal protein S3"
                     /protein_id="HOG15028.1"
                     /translation="MGQKVNPIGMRVGIMKSRPSEWFAKTKLQGADFFVEDIKIRNFI
                     EKKYSQSGIAKIIIRKTSKECEVIIFANKVGVLMGKQGENIKDMENELNKKWGKKFIV
                     SVKEVRVPEFSAKIMAEFISNQLENRMPYRKVAKNVLQKVMQKGANGIKISIGGRLNG
                     ADIARTEKFIDGRVSLQTFRSDIDYHEMQAMTKYGVLGVKVWIEKGTLYNKKKTLKKT
                     INL"
     gene            39701..40120
                     /gene="rplP"
                     /locus_tag="PK674_00380"
     CDS             39701..40120
                     /gene="rplP"
                     /locus_tag="PK674_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L16"
                     /protein_id="HOG15029.1"
                     /translation="MLLTPKRRKHRKQMIPSLKGKSTRGTTIAFGEYGLKAITNGYVS
                     NKEIEAARKVVVRHTRKTGRLRIRVFPDIPFTKKGLEMPMGKGKGDVDVYKAPVRRGK
                     VILEVSGLEKNLAKKILISASKKLGIQTRVAIKGKIQ"
     gene            40148..40366
                     /gene="rpmC"
                     /locus_tag="PK674_00385"
     CDS             40148..40366
                     /gene="rpmC"
                     /locus_tag="PK674_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L29"
                     /protein_id="HOG15030.1"
                     /translation="MKGKGAYIKQLNEKTIRELISERKKLKKELYNLKMKNAIRGLKE
                     THKIGEIRVNIARVNTVLKSKISIKPGQ"
     gene            40357..40611
                     /gene="rpsQ"
                     /locus_tag="PK674_00390"
     CDS             40357..40611
                     /gene="rpsQ"
                     /locus_tag="PK674_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="30S ribosomal protein S17"
                     /protein_id="HOG15031.1"
                     /translation="MTVIGDKNIKELIGEVVSDKTNKTRTVLVKSVKMHPIYKKRFVV
                     SKKYYAHDENNSSKMGDLVKIRECKPLSKSKRWYFIEIIK"
     gene            40645..41025
                     /gene="rplN"
                     /locus_tag="PK674_00395"
     CDS             40645..41025
                     /gene="rplN"
                     /locus_tag="PK674_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L14"
                     /protein_id="HOG15032.1"
                     /translation="MLKNESMVVVADNTGAKKAQIIRILKGSMATTASVGDFVVVAIK
                     SVIPNSHVKKGQITKAIVVRVKKEVPRKDGTYIRFGDNAVVLMTKNEKGEISPIGKRI
                     FGPVARELRDLGYKNITNMAEEVL"
     gene            41054..41344
                     /gene="rplX"
                     /locus_tag="PK674_00400"
     CDS             41054..41344
                     /gene="rplX"
                     /locus_tag="PK674_00400"
                     /inference="COORDINATES: protein motif:HMM:NF028446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L24"
                     /protein_id="HOG15033.1"
                     /translation="MKKLKSGDPVIVISGKYKGKISTIEKIDGDYAIVKNINVVKKAM
                     KGKGFIKKTLPIHISNIMYYLEKEKKASKIRIEITSDGKKIRKTKKGDIKIK"
     gene            41374..41895
                     /gene="rplE"
                     /locus_tag="PK674_00405"
     CDS             41374..41895
                     /gene="rplE"
                     /locus_tag="PK674_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L5"
                     /protein_id="HOG15034.1"
                     /translation="MAKDYKKNLQKRLNLKNINAVPTLETVIVNMGIGSIVTRKGHKD
                     FEEFEKNLSKITGQKPRIVKAKKSISNFKLREGMPVMLQSTLRGNRALDFIDRLYKII
                     LPRIRDFSGLNKRSFDSQANLTIGIKNYSIFPELGLDDVTIPMGIGITIVTSTNNQEK
                     SIALLEELGFVFK"
     gene            41987..42136
                     /locus_tag="PK674_00410"
                     /pseudo
     CDS             41987..42136
                     /locus_tag="PK674_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=25
                     /product="uS14 family ribosomal protein"
BASE COUNT        16159 a         6516 c         5525 g        13813 t
ORIGIN      
        1 aatgagaaaa ttatgatatg atcatattca tatgatgatg aggagcttat gctcaatatt
       61 tttgaaataa agagtatctc agaattgcca aagaagcaaa aaaactatga gctatctgta
      121 ttacacccat gatactaagc gcttccggag tatttaacgg gaaaacagtc acctcacgag
      181 atcaaaattg agttcaaaag aaatttattc aaaataattg aggattttgg gtagacaaaa
      241 atgtagtggt ttcgggaaac attatcactg ccaatggtcc agaatcagca aaagaattcg
      301 ccgaaaaatg tttggaaatg ataaggaata gttaaaagtt ataaagttaa aagtttataa
      361 agttgcaagt tataaagctc ttaataactt taagaacttt acaaacttta tgaactttac
      421 aaacttagct tcgcgaacag cacccggtgg taggatatga actttacaaa ctttacgaac
      481 tttttactgt ctagcttgaa aaactttcca acttttttat aaacattcta atttaccaaa
      541 aaatagtttt ttatgttaaa taaaaatttc agatatattg cactggattt tgaaactaca
      601 ggtttagatc caaaaaaaga tgaggccatc caaataggac tagcagaaat tgatgtaaat
      661 tgaaaaataa taaaagaatt taaatctttt ttgaaaccac aaaaagatat caaagaactc
      721 aaagattttg tagcttacat tacttgaata tctctagatg acttacaaga agcacctagt
      781 atttttgact tacaaaaaga gatttttgat tttttgggag aaaatgtgat tttgatatga
      841 cacaatatcc aatttgatat agatttttta aaaaaatatt tccaaaatat aaaatattat
      901 gaccaaatag ataccatgta cttagctcaa aattttgtcc attttgcccc tagttatgct
      961 ttggatgtat tagtagagta tctatttccc aaaaaagaat ttaaaaaaaa ttttcaaaac
     1021 atacaccaga ttccagactt tgattctcaa agtacccatg atgcattatt tgactcaaaa
     1081 aattcactat gcttatttgt ttatatagta caagatatct tatttttgat aaaaacctac
     1141 cccattttgt ccaattttat tcaaaaaaat ctatgattat atcacaaaat cttaaattac
     1201 aaagtagacc gggccagtaa aaaaaactgg aaattagaac taccaaaatt agaaaagcag
     1261 tttccaggcg agatttcctt aaaatccaaa gataaaataa acttgagaga gttaaaacac
     1321 aaagggaaat attttatatg aaatgtagac atcgataaaa taattgccac tatagtatga
     1381 tcaaataaaa ccataatttt atcctttgct agtgtagcaa aattaaatat cgtaaaaaat
     1441 attttgaatg acatgtgaat gaaaaacata ggttttgtga gaggaaactt aattatcaac
     1501 aaaacaaaat tcaatacatt tttaaataaa gaaagctttt ccgataatga acttttgttt
     1561 attttcaaat acatgtctca tgttagacac aaaatgtcta tattagattt aaacacaaaa
     1621 tttgatttca aaataaacta ttatattcaa gatgataaaa agaataaaaa atatccagtc
     1681 atactaacta ctcatttagg tttatttaca attttaaacg aaaaaaaaca tatttattac
     1741 aaatacgata tctgtttttt tgatccagaa atgtggtata gatcctataa ttctttttta
     1801 tcaaataact atgatttata ttcaagttta aactttttag aaactttata ttacaaatat
     1861 actttaaata atcaggaaag cacaaaagag cttttagaaa attttgcaag attttttgag
     1921 gttttcatgg gggttttgtt tgctgaaaca aaaaaacatt tcgtaaatat acaagataat
     1981 tatatcacat taaatccaat cattgatcat ataaatttcc atgaaacaaa tgccttgata
     2041 aaacaatttg ataagcataa aacattgttg gaagctgatt tagatagcga agattttaaa
     2101 aaattatggt gaggaataga taaaatgttt aatattttat ctggattagt gcaaataaat
     2161 aaaataatgt atggacagtc tgacttttat tttacttatt gagaagctac aaattttaca
     2221 aaccgagatg agtttgcaga tatctttgca tctcatactt tatttttgtc taatttcgac
     2281 aaatcttgta ataaaattat gaattcaaat gatatagaat atacaaaata taatcttaat
     2341 ttcaaaaaaa tttgagatat agataatgta ataagctttt taaacaaaga actatccaca
     2401 aattcagaca aaaccttttt tgtaatatct accataaaat tggaatctaa agaactcttt
     2461 gaaaaaatat atgaagcagg attggatcaa aaggctttat tattagtcga gaacattact
     2521 tgatctttgg gaaaaaatat tttcaaagca aaaaacaatg gtcccaaaat tattgtaggt
     2581 ggatacaatt tctttatacg attactatcc aataaaatca atattgacat ttgtgttgat
     2641 tttaatataa aatgaaagtt gtcagaatat cttttaaatg atatgcaacg atatgctaaa
     2701 tataaaaaat aataatctat ttattctgct aaagtttata tgagctgtaa taatttttta
     2761 tttatgaatc aaatttagcg tttgttttgt atgagaaaaa acaagtatag tagatgaaca
     2821 gctatataac acatgaacta gcatggataa tttagacgaa tctcggaaag atttattgga
     2881 aaaacatgaa attgctgaaa cagagccaac agaaactata gatgattaca aagaattaaa
     2941 agaagaacaa aatgaatatt tattgaaatt ccaaacaatc tgctcttcca atgcttgatt
     3001 atgtgcaaaa atcaagtttc aatgagaatt tgatgcacaa gacaaatata tgtatttagc
     3061 tagttctttg tatgttttaa aacatataga aaataaccaa caatttggga aggctgtaaa
     3121 acaacaactc aaggaaatta aaataaataa tgaaatagcc agtagaagat gattcgctgg
     3181 ttgggatact gttacaataa atttatgaat ggtaaaatcc tatgttgagt ttttggaact
     3241 tattactcat gaaatgtgac atatcgtaga tttagggatg attagatgat tcagcaaaca
     3301 aaaagactga aattacacag aatttggaaa tgaggttttt gaaatagatg atccttctat
     3361 aatgttttat aaattatctc gacaaagtga aaaaattaga aaagctgatg caagaaaaga
     3421 tgatttttgt agtagctatg gtatgacaga cccttttgaa gatttcgctg aatgtcataa
     3481 tttatactta aatcacaatg ctgtatttgt ttatcgagcc aaaaacaatg aaatcatgaa
     3541 acaaaaatac aatttcattg caaatctata ttgatgaaaa tatctatttg attctactaa
     3601 agacttagat agagcaaaaa caaattgatc atggagacct tgggatacta ccaaaatgta
     3661 aaaactttca ttaaaaacat agaaataaaa catagaaata atgtgtttgt caagtcaaga
     3721 ctaacttaaa agttagtttt tttttctttt tctatgattt tgcttataat tcatgtagtt
     3781 ttatagactt ctttaatata aatatgaaaa aatcaaaaaa aactataata tatttaatta
     3841 taataatatc tgtttttgtt tttcaaaatg ttttttttaa cattaaatta tttgcagacg
     3901 acaatgtaaa agtcttggaa gcaatagcag aatcctgtat tgttcattga atatgataca
     3961 gctttgacaa agacaaatga tgtgtttgtg ataatccagc aaaagatgac aaatgcgaaa
     4021 atggttttgt aaaaaacaaa tattgatgtt gtatcggatc ttgcggagtt tgttcttcaa
     4081 attctgtaga atttcaaaaa tacataaatt ttcaagtaga actatatggc ttattattag
     4141 aagaactaaa ccaagataaa gaaaacaaac aaagtggtgg ttttagacaa ggcggtttat
     4201 ttagttccag agtgttatct attccagtaa acatacgaaa atctcgaact aaaatattta
     4261 aaaaagttgg acaggaatat attgatacaa aaagcacaat agtggtgtga agtaccataa
     4321 ttacctccat agtaacagaa tccagaaaag acatgcttgg tggtttgatg attttgtaca
     4381 aaagtaaagc tctcgtcaga gaacgaaata ctatacaaag tttagattta attgtttacg
     4441 atttaatgcg agaccttaat actacatgat tatggaataa ggaaatttct aatgatacaa
     4501 aaataaaact agaacaatta aataaaaaat acatcaaaac agaacaaaac caaaaatgaa
     4561 tgtttgaaaa attttatctt caatgaagca taaaatataa agatttaata aatgtttcaa
     4621 aaaagatcaa ttctttaatg aaaaatttca tattaacatg aaaagaggga gactctttta
     4681 tgaaaagttt tgaaaaagag ttttcaaaag gaaatattgt agtacaattc agcgaacatc
     4741 gaaaaaacaa tctaataaat gattatgttt gtgctagaag gattagtgct tgtaaagaag
     4801 actttagtca ccttatcaat gtaaattgat taaaaaactc tttttcaaat tctatgagca
     4861 ttatcaagca ggcgaataaa aatttagcag aaatttttac tgataaagaa aaacataaag
     4921 ataaagaatc aaatccttat tgattgacag acaaacaaat cgaactccta agaacaatat
     4981 atggaataaa tacatctaaa ataactcaac aacaaggagt aacattaggt tatgttttta
     5041 aaaaaacagc acaagtatat aaccaaataa agaatcaagt aaatcttaaa atttctgact
     5101 atgctacaaa aaaacaagaa caagaaaaca aaaaaaatat aaaaaataca aaaaataaag
     5161 aacaagatga aaaatatatt aacaaattat caaaagaaga acaaaaaaaa atcgaaaaag
     5221 ctttaaaaga acaaactgaa aaaatagaaa aaggagagac aaccatagct tttacaaaca
     5281 tcatagaaac tattttaaat gaaagagatg aaaataaaga aataattctg ttttatgata
     5341 ccaattctac caacagatat ttcacggaaa caatcggact aattcaagat atcattaaaa
     5401 atgatatatg aaacaaggat acacagggat tagtgaaata tctatgagaa gcctgcacat
     5461 atcaatgtgc aaacaaagga acgacaaatt gctttaaata atacttatac aaaatgaaaa
     5521 aagaaaaaac tataattaaa acaatattaa taatactact agcaaatctg tgagtttatt
     5581 tttcgtttgc ttcagcttga aatcttagtt gcaatagtat gggtttgcta ttaagtgcgg
     5641 atcttaccaa gctatccgag ttatgaagtg ttaaacttat aagtcaaaat ttttgtgagc
     5701 atgtacacaa ttctaaatgt gaagataata ttgatgctca tgattctgac tattttgatg
     5761 caaaccaatc tatcttttta actctactat gtgataatgt gggaaagggg caaaatttta
     5821 catttataaa cagaactgaa aatgactcta tcttaaagaa aagagccttt tctgatttca
     5881 acatttataa ttataatatg gaagacataa actattgcga ttcaaaaaca aatatgaatt
     5941 gatgcgactt tcccaaacat ttaccaaaaa tgtttaatga aattattaat gattatttta
     6001 acatatgaca agctagaata tatggtataa agtgatttca agaaaaaatt aatcctatta
     6061 tttctgcaaa cgaattttct gatgaaaatt ttcttataaa aatttgcgac ccaagcaaca
     6121 aacaatatta tacaaaatct tgtaagtatt taagcaacta catgaaaaaa gctaatcaat
     6181 tattgataaa aaccaaagta gttgatgtca aaaaactaac agaacaaaat aaaaattttg
     6241 attgtgataa taagttttca gaaaacattc tatattgtgg tttgttggga agtgacaaca
     6301 atcttagtga taattttcta aatcttattt ataatgaata tttatggtat cgcttattta
     6361 tggaatatta tagcacaaat ctatctacaa acccagacct atcaaatcta atatcaaact
     6421 ccaatgcaga aaaattaaaa gacaacaaag aaaaaataat ttgattacaa caaaataata
     6481 cgaaaatcaa aaaagctcta agtgattcct taagaacctt atcagaaata gctcaaacct
     6541 tcccactaca tatctgattt acaatgtatc aggaaaatgc aaatatattt atgaaagaat
     6601 tatctaaaat ataccctcct attaggactt tagccgataa acttaaaaat gttcaaaaaa
     6661 cagactaaaa attacataca caaaataata aaatcaaaaa atataattat atttgtattt
     6721 ttaatggtat tttttattga tttttcattt tcatttgaaa taagtttaaa agattccata
     6781 gaaaaaattc aaaatacgat aaaaagaaac tcagaaataa gtattggatg aaaagcaata
     6841 atagatgata tttacaaaac atctctaaac aaaatacaaa tcgacaatat taccccatta
     6901 ttaatagcta cagacaaaac agcagaaaaa ataaacaaag actatacttg tacaatgaat
     6961 aatcaagaaa ttatagaaat actatacttt acaaatgctt catttaaaca tgagctaaaa
     7021 aataatttag aagattttaa aaatccaaca aaggaccgta tttgaataaa ttgtaacaaa
     7081 ttaaacacct gcatctacaa agacaatctc gcaccatgac aagtttttaa aaacacggta
     7141 tcatccaata aatcatgcca aaatattgta gaaaacatat ttttgacatt ttatatagat
     7201 tcgtattata acgggaatat atgagaacag ataagatgaa cagacacttt ttggaacaat
     7261 gatttaagtg atagtaacta cgatctatta aacgatatct ttaaattggg aaccatactt
     7321 tttcaagata taaaaagccc tgctaaaata ctattttaca gtatgccaaa aataaactat
     7381 aacaacatat atcaagagcc aaaactaaat atgaaaattg attggttttc tccatataat
     7441 attttttatg gtgatgaagg gggaaacaac acaaacagcg gaaatacatg agatacatcc
     7501 caaataaatt catgagacaa tttatgaaac aacttatgaa ataatttacc aatcatcaac
     7561 gaatgaatgc aaaattgatg aatttcatga gagatagaaa gctccataaa aaacttcata
     7621 aattccgtaa atataaactt aaacaaccct tcttcatctt ttgttggcaa tcaatgtgtt
     7681 tctggttttg aatcagaaaa atatatgatt tcattttcta cagcagactg agaaaataca
     7741 caaacaactc cacaagaata tttagaatct gtgattgaag aaattgagga cttaaattgc
     7801 aataacgaca gtatttgtca agatcgagag tctgactctt gtcctgactg ccaaagttct
     7861 tcttatgacc cagaagatcc gtccagtttt gaagaaatcc aagctatttt agaaaatata
     7921 atagaacaat gagaagaaat accggaagat gatccagtac ttggatgtgt acaaaaatgt
     7981 gatctaatgc catgtggagc cacagaatgt cctaaacttg tatgttatgc caaatgtttc
     8041 tgtcaaatat atagttttga tacagatgat ctagatttaa cgagcgataa cacaaacata
     8101 acatgattaa ataaaccaat tagatctatc ggtttaagca gtaaatttgc aatcaaattt
     8161 tgtataatac ctgctatttg atgagaagta caaactaaaa caatttatag catcactagt
     8221 atattttctg aattaaacaa tatattagaa aatctaaaaa acagtgggaa tatgtgaata
     8281 aacataaaac caaaagaatt tttggagcac acaaaaaaag aatataattt tgcaaaacaa
     8341 atatctttta atataaatag cgccaaagaa cccgtatcct caaatcaatc taaagattga
     8401 atgagtaagg agcaaataaa tctaaataca gacttaatgg aacaaatttt atgattttcc
     8461 aaagaatata atacagataa agagcaaaac aaatatattt taatagatga tccatgtgaa
     8521 cataaagtca ttgtccaagc aagtaataga gatgaaaaag aagctctttt ggaatcttgt
     8581 aggcaagaat ccaatatgaa tccaacagta aacaacgcaa atctaaaccc agctatgata
     8641 aatcaaaaca taatgttgat aaacatagag tttgatgatt ttatggaaaa aaatcgtgat
     8701 ttttggtatg aaaccaaact catgtttgat gcattaaaaa acacggctca aagtttggaa
     8761 aataaaaagt aactcaacaa caatcaaaga caaagatatt ctaaaactta gatacaaagc
     8821 tagtgttttc aacaatactg tttgcttgta aattaaataa attttattaa aatgaaatga
     8881 agtttttgtt ttaattattt ttttataccc atgaatgata attatttttc agaaaaaata
     8941 acggtagaac caaatcacaa aagacaaaaa aaatccgttt caatttctac tcttataatt
     9001 tctataatta ccaccatagt ctgtgttttt attataagca gtaaacgaga aaacataatt
     9061 tctcttttct gattcgactc tcaaatacaa gaacaaaaca atgatacaca aacaaatatt
     9121 ggcgacgaaa tatcttgtac ttgaaaaatg tatgacgact gagatatttt aaattatact
     9181 cactctctcg aatctttgga attttgaaaa ttagctttaa aaagttccaa aataaacctt
     9241 aataattata aaaacgaagt ttacatagaa ggaatagtcg aaaaaatatt tcaatgaaca
     9301 ccagttgttt cggtatctac catatattct ttggatatag aagaattgaa tgaaaatgaa
     9361 gatgaaaacg aagaaaagct attagaaatg gactacgaat caaagttctt accaaacatt
     9421 ggaatttatt tagataacaa atttttccaa aaatattcac tattaaatga atgagaagct
     9481 tgaattttaa aaataaaaaa atctgaaacg aacgagataa tcaatattaa ttattttata
     9541 tgttccaatt ctgataataa taaaaattgt gacagattta acaatatgtt ttcaacatct
     9601 tcaactgaga aatttgtaga cacttacaat ataaattatt acaaacaagc agaaagccaa
     9661 tctcgatttt tttctaatga ttcactattt ggatatttca taaacaatac tgatgatttt
     9721 ttaacaaaag acttaataaa acatatacaa gtaataaata aaaaatttat agaaaaaaac
     9781 attttaaata aggtggactt attatgtcaa gattccgaaa aatgaattaa aaaaatcgag
     9841 gagagctcta ttgtactcaa agataaagat cttttggtta atttgaaatg aagtaatgaa
     9901 gaagaatctt ttgaatgtaa attaaaaata gatcctactc taaaaaacat ggcagcactc
     9961 cttgagttaa agtccctctg aaaattggaa aataaaaaca caaatgaaca aacagacggt
    10021 aataaaaaag aagtaacagc aaactacaat cgagatccca atatcccaca attccctata
    10081 aatctagaaa aatcattaaa atttacctct cgtaaaggat tttcttacac tttcccaagt
    10141 agtaatattg cctatgccgc acaaaacaca caagaagatt tcggacaaat atgagtaaat
    10201 tgttattcag taatgaatgt ggtccaatat tctgaaaaag aattagtaac ccaaaaatga
    10261 gaagtaaaaa tatatgaatg taatataaaa ggagattttg aagaatccga gaaactaatc
    10321 cacaaaaaag aatgagaaaa agatttcata atagaaatag taaaccctgc acgaatcgct
    10381 tttgcaaaca atattacaat tagtattgaa taataaaatt ataccccaat tattcttcta
    10441 ttttcacaac cttattatac attttaaacc aaaacttcct gaaaaaagct tgtattatag
    10501 aaatcatata tattatcaaa ataacagtaa ttatccctat aagtcgtatt acccgtttaa
    10561 aaccgcggtc ggctaatcat gcatttatca aaaagtacac caatagcata ggagcggagg
    10621 ctattataaa tgctttgaaa taaaataaaa tataagtcac caactgcatt actcgtcatc
    10681 ttattgtaac tcaaatattt gttatagtta ttgaaatact cttttttata gcctcaaaaa
    10741 ctcacatatt ctctataact atcaaaaatt ttgcatattg ccaaaaaata ctactaaata
    10801 aagctgccaa tcaccaaaca ctaacaagta ttatagctat ggtattatct aaaattcata
    10861 aagtaaacaa cctcaaaacc gtaatagcat aagtataaaa tcaaaacgaa aacgctaaag
    10921 catttaactc aaacataaca aaaaaatcag taactccttt tcatatagct tttcaaatac
    10981 tatttttttt atctttcaaa aaatgtatta ttgctgacat tccagtagga tataaaaaca
    11041 catatcatat taagatgata aaagcaatca atataactaa ccatactaaa tgattagata
    11101 ttatttcttc taaaaaatac tttaaagtac tcattcgagg caatcactta tcaaatctat
    11161 atattaataa attattaata ttataagaaa tcaaaactaa agtataaagc aattttcaaa
    11221 aagatatata aaaaaccagt tttataaatt tattgttatt ttttatgtaa tccttagatt
    11281 ctctaagtaa acttaatatc attttattta aactaaacta attaataatg ccccataata
    11341 gttcttttgt aaaaatattc aagcaattat taaagagcaa ttaattgaaa aaaaagtagg
    11401 tattatttgt aaataaggat attgaaaaaa atatttattt agatataaat taaacaattt
    11461 agaacaagaa cacataatga ttgtattaaa agttatagat aaaattttgg ccgaaattat
    11521 agtctttttg ataaaaatat atcaatttac aatatcacca gataaatgat taccatcttt
    11581 gcgactaaaa tgaaaaattt gttcacacta ccctcattgt agtcaataca gtataaattg
    11641 tctcaaaaga tactgattta ttaaatgaat tcccaaaatg acagaaagag ttgtttcttg
    11701 tactccatcc aaagaaaaaa tatacgaccc agaatactac aaaatagtgt ttttttcatg
    11761 agcaccaata tgagtgccat ttttgcatga attacaaaaa gacaaaaggt ttgaaattgt
    11821 atgagtagtg actatgccag atgctcctag tggaagatga atggaagcaa aaccaaatat
    11881 aataaaaact gaagctgaaa aaattattgc taatcacaaa gaaaacacag cgaacgaatt
    11941 catcaaaact cctaattctc ttcgtttaga tcccaaaaaa cactctgaag aagcacataa
    12001 gttcaaacta cgacttgaag ctaagaaccc agattattta gtagtaatag cctatggaaa
    12061 aatcatacca caatatattt tggacatagc tcaaatagca ccaatcaatg ttcacggatc
    12121 tttacttccc aaatatcgcg gagctagtcc actgcaatca gtatttttaa accaagaaag
    12181 acaaactgga attactataa tgaaaatgga tgcaaatatg gatactggaa atatgataga
    12241 tatgctcaag tttgatatcg aatttaattg gacagtaaaa gatttaatag aaaaaattat
    12301 gcaaaaatga cctcagtttt taaataatac tttacaaagt tttggaaaga aacagctttg
    12361 agaagtaaaa caagatgaga ataaagctac ttattgccag aaaattgaaa aagaagattg
    12421 agaaataaat ccctacaaag attctattca ggaaatttat aataaatata gatgatacta
    12481 tatacgacca aaaatatttt tccaaaattt aagcactcaa aaaagagtaa taattgaaaa
    12541 cctaaagcta gaccaaaaac tatttgaatc agaaaaaaat aaaccattat ttattgaaaa
    12601 cgagttaaac aaatgtattt tggacatatc tctaaaacca gaatgaaaaa aactaatgga
    12661 tcgacaatca tttaagagcg gatatttaaa aagcagtaaa taaaaattat atctaaatat
    12721 tatcaccaaa tgttttttca ctcactaaat tcacacctga atgaaatgaa aaatcacttt
    12781 gcaaagagat tatatgagaa caaattgctt gtacaaaata ttttaattac taaactaaat
    12841 taatgaaaaa aagattgttg tttcttacat tggttttatt catatactga tgtagtactc
    12901 caatacaaaa cactacaaca caagacatcg tgaagcaaga tactagattg aatgagccaa
    12961 actattctat aaagacagaa tctatggtta aagtagagcc tgaatttatt gataaatccc
    13021 aagaaactcc aagttttgaa gagatagatg atatagtttc agtatgccac aacaatacag
    13081 ctaattgttt gatagattgt gatgattgga atattacaaa aaaatcttat attatacctt
    13141 ataaaaattg atatataggt tttgattatt gatgaaactg atgatgtcgg tgatattttt
    13201 taacatataa gtctttggat aatccttgtg atgaaacaat atcaaacaca gatgaaatat
    13261 ttattcgaaa tgtaagttac ccaaacgatt tagagcgatt ggactctcct gaatatccag
    13321 acaataagtt atattatgct caatgatgat ggagttgaat tcctattgaa gaaattgcta
    13381 aacacttaaa ttgtaaacca tgaaaacctt ttgaacaaga cgattcctgt aaaaaagaag
    13441 ttgatagatt tatgtataat ttgataattt gaaatgaaga aaatgaatac ttcactcaat
    13501 ggatggaaat gtttaaaaaa aatttagata ataatatatt tacaacacgg tcttttcgat
    13561 ctgaaaggtg gaaaatttgc aataaaagat tctcattcga atctctactc caagaaaatg
    13621 gaaatgataa tagaacactt tccaacgaag taagaaaaaa atataataaa atacaaaacg
    13681 aaagaattca taaatgttta attgaatatc agcgaaaata aacctttata atctcaaaat
    13741 accaaaatgt ccgaattcaa aaacccgctt tctcgatcaa tttctaaatc tactgtttta
    13801 catttttgta aaaaaaaata ttacttttct acttattcaa actatttaaa tgaagtagat
    13861 aaatgactat ggatccaagc attattagca aaaaatttga aaagcgtata tatgcgattt
    13921 ggagaaaaaa tgcacgatct tatatctgat tatttgaatt tagtgaaaac ttgagatgat
    13981 aatttagata aaataaagaa aacaaaagaa gatttaatta atcaaatgga tcaagagtat
    14041 agtgtttcca aaaatagaga ttatctaaaa tacgatccaa atgtaaaatt tggtctaaca
    14101 gagcattatt acggctacaa cattgataac atttatcaaa actgaaaaac aaaattactg
    14161 gaagattttg ataaatttat aaaaagcgaa atcaataaag aagtaaaaga atactttgaa
    14221 gacaagtcca atattctctt tattgaggct aaagaaaaag attttgaatc tatgaaaatt
    14281 caaatagaaa acataccaga attaatgtga ataaatgttt atgctcaacc agatttttga
    14341 atcatcacaa ctaataagga ttatataata tatgatcgaa aatattgaaa agttccaaat
    14401 aaagataaaa gcctagtatc aaatcaacta aaagtttacg catacaaaat attgcaaaaa
    14461 attggaatag aaaatgtaga taattttaac atcaaagggt ttgaagttta tctaaaaaat
    14521 ctagaaaaat ttggttgaaa aataactaaa caagatttgg aagatataga aaaaacaatc
    14581 atatcagata ctacaataca aaaacagttt ttaataaatc aagatgtaga aaaaaatcag
    14641 ccactaccaa gcgaaaactt tcctagaacc aatgatttac aaaaatgtag taattgtact
    14701 ttctacaaag tatgtgaaga actaaaaaaa ttcgaaaaaa cacataaaat agaagaaatt
    14761 aataatatag acaattctgt aaataatgat gatgagtata atgaagatgg cttccctttc
    14821 taaaaaattt cttcaaaaac aaaaatacca acaatggtat ttaatttagc cgtcccgcag
    14881 aattgcggga aagagtggga actactccta ctataacagc aggattgctc taccgacaac
    14941 ccttatcata accaacaata aaaaggttta tattttcaaa tcttattgat cttaatttag
    15001 ccgtcccgca gaattgcggg agaacccggg accactcccg ctataacagc gggattgctc
    15061 taccgacaac ccttatcaca attaacaata aaaaggttta tattttcaaa tcttattgat
    15121 cttaatctag gccagccggg attcgaaccc gggaccaccg ccttaaaagg gcgttgctct
    15181 accgactgag ctactagcct atttctcctc acaaaaacga agatcaccat aaagattgat
    15241 ttaaataaat caacgcattt ttttatacaa tttcgaatca actttaaccc ttattttagt
    15301 cccatcatct aaaactaccc atttatatat taaatttggt ttaaatgttc tttttgacgc
    15361 tatcatagaa tgagatcttt tatttcaaga tttagttcat ttacctgtaa aatcacatat
    15421 tcttgccatt ttttaattga ttgatttatt taaaatatat taaacactag tttcttctga
    15481 tgaagtttcc tcttctgaag gggaatcctc tgttcattca gcacttactt gttctgtagc
    15541 ttctgtagca gcttctgcgg ctgcctcttt tgcttcttct tcagcagcag attttttaag
    15601 atctactaca gtaattactg gtaattctca atcttcttct gatacaattc cttctggaag
    15661 gactaaatct ttaacaaata taaccgtatc taaatcagta acattagaaa catctacagt
    15721 aatttctttt actaaatctt ttggtaaagc atttaccaac aaataatctt ttaaaacctc
    15781 aaaacttgca tctcaagatt ttactatagg agataagtct tctcaagtca taactatagc
    15841 aacctctgct gacactttca ctcatttttt gattcacaaa aaatcaatat gaacaacctg
    15901 atctctaaca ggatccagct gaatatcttg tatcaaaacc aattcgtcta ttcattctca
    15961 gatcaaagta attggagtac tatatccagt ttgtctgtat aattttacaa attcatttct
    16021 atcacacaat atagacacag gtttttctaa atgtttagaa taaactaccg caggtacttt
    16081 atcttccctt ctaagttttt ttacattttt tcatactata ttgcgaaatt ctacatttaa
    16141 tttcatgatt taacttgttc aaaaaactaa aaaaacatcc tttattttat tcctctcaaa
    16201 gttcttctga ctctacatct gcctcgttaa cttcttgatt ttcaactttt tcttgagcat
    16261 tatctccaat ttttttttca acaatctctt caaccttttc ctcaataact tcttccacag
    16321 aatcgttgaa actttcttct actttctcct tattttcaac aaaaacttta acagtatctt
    16381 cttcattttt agtcttctct tcaacaactt tttgttcatt atttttttct acttttgtat
    16441 cttcttttac ttgtctcaaa ccgtctaata aatctattcc ttttgtttca gttttgacca
    16501 aactttcaaa tcttgcatcc tttccaactt tatctctaat ataatataat ttggatctcc
    16561 taattttata attatccaat aatatcacct tttcaaaatt agggaaagac aatggataaa
    16621 tcttttctat agtcattcta gcgacttccc ctctaatagt aaaagttccg tcaaaatgat
    16681 taggcttttt taccctgatc actaatcccc taaatttcca aattctttta gtgtctcatt
    16741 ctcaaacatt ctcatgaatt tccaaaaaca taccagattt tatcggtaaa ataaacttag
    16801 aatgcttttt ttgaatattt tttattctct cataattcat tacctatttt ttatcaacta
    16861 ataaaaaaac tatatttatt acaaaactat tttttactat taacaacttt agctttccat
    16921 tttttttgtt gctccaacat ttttctagta tccataatca caaccttttc tactctactt
    16981 agagctttat ttaacttttt tttacccatc gtttttaatc ttcacattac gctactattt
    17041 tagaagataa atcatttaat cattttctat ccaaagcttc tttaacctga ttcatcgtaa
    17101 ttcaatattt ttttaatcta gccaaagctc tttgtttagt gtcttttgtt cgtttagttt
    17161 ttcatcaaat agattctttt tttaatacca aaatgtatcg ttcaatagcc tttttatatt
    17221 tttgtctaga ctttttcttt ttccaattta gtccaaccaa aaaacgtttt atttttcttc
    17281 cactcgtatc tcacatatta caataagttt aaattttaaa gtataatttt atatagaaca
    17341 attaattgac atattttgcc atataagcaa atgctttgtt ggcttctgcc attttatgag
    17401 tttcttcttt tttcttaaca gccgctcatt ggttagaata agctgccaac atttcttccg
    17461 ataatttttt gtacataggc tttcagctct ttgatctaac tgcatccaat atccatctca
    17521 tagcataaaa taatcttttg ttttgtttta cctctactgg aacttgataa acagaacccc
    17581 ccaatctttt tgatttaacc atcatcaaag gagaagcatt ttctattgcc gtttctacga
    17641 ctatttgagg attcatatgt cagctagatt taatgtcttt caacatatca ttataaattt
    17701 tcttggccac agatttttta ccatttttca taagataatt tataattttg tccaattttt
    17761 cattggaact cggtaatact actttaattt ttttatttaa ttttgctacc atttatcaca
    17821 aatataattt aattaaaaat atttatatac taacttaaac tttacaaatc tcgccccgct
    17881 atattattat cacaaaataa gcaaacttta tgaacttgat aaacttttta actctttgat
    17941 ctttttactc catacaagga tctaccttgt tttctttcgg gaacaggatt tgcatcatat
    18001 tttcctctca taatatgata tttcactcca ggaagatctt ttactcttca tccttttacc
    18061 aaaacaacac tatgttcttg taaagtatga gattgacctg gaatatatgc caaaacttct
    18121 gctcaattgg aaagcctaac ttttgcaaat tttctttgag ccgaatttgg cttttttggt
    18181 cccattactc aaacttttaa acaaactcat ctcttaaaag gagaaggcat ttccactttt
    18241 ttattatttt ttaatttgtt gatagaaaat tgcaaaactg gcgattttga tttctttttt
    18301 ttagcttttc ttcaattttt aattaattgg ttaattgtag gcatattcgt cattttctaa
    18361 aaaaattaaa atgacattta aatgtcatca tgtgaaatca gtataaaaaa ataaaaacaa
    18421 aaatcaagtc aaaatgtaaa aaaagttttc tcattaaatc aaagtccatt aactatttta
    18481 aacacaagta taacttttca tacttttaaa aaataaaaaa catagcatta aaaaacattt
    18541 caaaaaagga acagtttttc actgttcctt acttaatatt gtaataaatt tattttctat
    18601 acacatccaa aatctctgta aatgaatctg ctcatggagg caattgtgtc cataaagtag
    18661 gattatattc tattaacaac gcagctcatt cgaatatttc tctttttctt tcccttttaa
    18721 tagctgatgg tgaactactt tttacagtaa accaatcgtt taaatgagat cttctattat
    18781 ttaccaaatt atctatatca tcacctatca aaactcattt tacatgtaaa ttaccccgag
    18841 gacatcgttc ttgattttta cttgtattcc tagttcgact tcttgaatca aatcatcatt
    18901 gtgcaataaa tattccattt actacttgcc ctccttcttc acaatagtct ttgtcaccat
    18961 catcttcaaa agaaatttta tctgttgtga taatcattcc gtttgtagta atagatcact
    19021 ccaccaaaac atccattcaa tctatatata cagtaaatgg aacagatgga aagtattctg
    19081 tcattttttt caacactaac tgaccatctc attctacaaa aaagatcatt tgatttggaa
    19141 cttttttcac agttgaatta ccaaatatat cgttaattct aacattatct cttaccttaa
    19201 aatttccatc taccgcaata gccaaagatt tatatttatt tttaaaacta ttcatctgat
    19261 tcaccacctc actatcaaat ccatatgtcg cagaatctac tgacatagtc tcttcaatat
    19321 ctgtagattt tatcaacttc tctcacttca tatcataaaa atctttcaaa aagtctgcac
    19381 tagtagctac aggacttatt ccaaaagtgc ttatttgcat aatatatggc ctagttagtg
    19441 caaaatccac ttcacaaatt cacttataag ttcttcccgc tctccaattt ttggttgcag
    19501 gatcacaata attgtaatcc actttttcca aaactaattt atattctcaa taagtatcaa
    19561 ccgccccatc aaacattact atcgtattaa aaacataagt tccagcaacc ttggtaaaat
    19621 caattttata tgccttttca aaataattaa atattactga attatttttt tcataacaat
    19681 ctgtaataaa tgtatgcaac ggattatcat ttatttgttt atgcttattt caatcatata
    19741 cagagaaagt acatttcatc gagtttttat ctatataggt tataatctca tcatcatcta
    19801 tatttgaaca gttataattc gtaacaaact gttcttgcct cttccacaat ctccaccaaa
    19861 atggcatcaa ctcattttcc atcaaagaaa ttgatcagtt tacccccaaa cactgaggtg
    19921 gttgataaac aaattcatta gtagaatact tcctaattct acattcctta caataataat
    19981 ttcagttacc ataaattcaa gctaaataat tatgatcaag atctaaataa tttcatattt
    20041 ttattctatt agatatcaaa tttacatttg tccacaaatc acattcttct cctatatcca
    20101 aagttccatt tccacaaaca cctacatatc aaggatccaa aagaacagca gttttacatt
    20161 cgtttctagt atgattgtcc acataacacc ttacattata agtgtttcaa gttactcaag
    20221 catttggatc ataaacataa ttacaagatt ctgtaaacga tgatgcattg taaccagttt
    20281 taacaactcc atttccacaa tttaatacta tctcatccgc aagtactcca ttttgagagt
    20341 aacaagtaaa tctcctagat cattgataat tatcatttat tataataatt ccaccagtgg
    20401 taatacggtc acacaaagct ttttcttctc aaactactga aaaatagtaa tcagcctgat
    20461 ctgtataatt ttttcataat tcggtatatt ttaaatgaac cttattaata tattctggat
    20521 ttatacagct taaatcatct gaaatatgtc cagatatgta taaggttaaa gtttctcctg
    20581 aatccaagct aggataactc caaaccaaac ttgtagaatc tttaactaat ccagcagtac
    20641 ctgtccaagc tacatatgaa atacaatctg gatttggcca aatatcttgt aaaacaatct
    20701 ctgatatttt atttggtcat tgattcttta ctgtaatttc aaacaattta tcatctcata
    20761 acaaatgaac atcaggatca gaatcctttg tcaaaacaac aatagatttt tgtgtcctat
    20821 gtgcagtagt actggaaact aagacattca tcggattata aaaatatata taggaattat
    20881 ttacaaaatg tgtatatgca ctacaggcta cagaatccac taactgaccc gtaataatca
    20941 catatcatgc ctgtccagga ctaagattaa atcctgaata ttctataata taccttccgc
    21001 tactatcttt ggtttgagca aattttgctt tattaactcc cataatccta gatgacaaat
    21061 aagaaataca caaaggcata cgatctacaa tacgaactca catagcatta ccaactcaaa
    21121 gatttgcaaa atcaatttta tatgttatca aatccccagc tttaatattt atattccctg
    21181 tagtaaacgg aatatttcca acacgttgat atttttgtat tgctggttgc ggttttagtg
    21241 caaaataggc agtatcataa gctcacgaag taaatgccca gtttatagta tgataaggag
    21301 aagcatctcc tttaacctgt caggtcaaat acactatcgc actctgtcat ggctgtaaat
    21361 taaatcatga atattcaaac acccaatttc atccactcaa ccactgacca cttttatatg
    21421 gcgaagatat tcattgaatt gtatgagata tcaacttcaa tgataatggt aatagatccc
    21481 ttatatatgc attagtaaac actcacgaac caatatttgt taattgtatc ttaaacccaa
    21541 cataatcccc agaactaaac tgccctgtat tcaaaagagt ctttgtaatc cctagttcac
    21601 caggagaagt tggtttcagt agaaatgggg ctgaggccat tgctcatgat gtgaatgccc
    21661 agtttgtttg caccatacca ccaaacatag atggctcagt tctaacttgt caggtcaaat
    21721 acactatcgc actctgtcat ggctgtaaat taaatcatga atnnnnnnnn nnnnnnnnnn
    21781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21841 nnnnnnnnnn nnnnnnnnnn nnattgtatc ttaaacccaa cataatcccc agaactaaac
    21901 tgccctgtat tcaaaagggt ctttgtaatc cctagttcac caggagaaac tgatggtaaa
    21961 actttcaaaa ccccagtaca agaaaatgct tgaccatata tcgtatgaga atttattgaa
    22021 aaagtatatt ctcaagtatg acttatattt ggtcacacaa aaacttctca agtattatag
    22081 taaatattat gtggccatgg ccccagcaaa gcaggattaa ctaccataaa tgctggaata
    22141 ttaaagtctg gccaaatttg ccaagagatt tttgcaattt ctcataactt tataatgtgt
    22201 ggagtagaag ttatagtaca tccagacatt atttggactt caccagtaca caatactata
    22261 ggtctagtga ctcatgttgc aaccgcacca ggatagttgt ttttagctcc ataaacaaca
    22321 gtataatttc aaattttagg gtacacatat tccacatatc atcctgtagg ataaattata
    22381 ttaggtgttc atcaagctaa agtatctcaa agattcaaaa aatctgtaat cgcccaaggg
    22441 tttgtggtca tagtaaaagc tgttggatat caaagaattc aaagaacagg gtttacagtc
    22501 aaattacaat taggagtcac caactgacat tgattattac agccgtttcc aaaattaggc
    22561 ccattatcac attgttcata aactccatta tcatttggcc gatctacttt cccatctcaa
    22621 caaactttta gctcaggttg aggaatatta taaacaataa tacaagtatt tgagccagca
    22681 gtattagcaa cactcatagt agctgaaaaa ggtccatagt atccacttgg agctacggta
    22741 ttaaaactac cgacatttgt atgtacactt ttataattag gcgaaaatca aagaggttga
    22801 atatttattt gagttggatt aaaacttcaa tttactgtcc aactcacttt ataggtagaa
    22861 ttttgtagag aacttataga taaagaacaa gtaggagctt cttgtgtaac agctttacat
    22921 gtgttgctac atcatgcgtt tcatcgacca gcatgagttg gatcatttgg atcacactcc
    22981 tctacataag tcccattcgt ataactcgta cttccacccg gtctatttaa aattccatct
    23041 ccacaccaaa aaattctata attcaaacat tctcagatat gtgaattgag aactcaatac
    23101 tttactgtta cctttggaca agaatcataa tgagaaactg tatttcaggc aggataacat
    23161 gtccaatcat tttgtcaata atccttataa aaaaatttat tcttagtact gtgcggtgct
    23221 caagcaaaaa ctttatgata ttttatatta aaatgaattt gtgcatctat tttatttgga
    23281 ttggaatact taagataatt tggtttatta aatgtacagc catcataatt ttgcacagcc
    23341 aaaactcaat tcggatcatt tgcaactgta gcattactaa ccctattcca atcgctacaa
    23401 ttagtcaaaa cattaccatt acgccaaagt tctacaaagg tatttaaaga tatcaaagaa
    23461 tctaaagtgt ttcaaaaaac atcataagga tttctagtta aactatttac cccattgtcc
    23521 aaataataat acacatcttt tgattcattt gaattacacg aattataaat agatgacatg
    23581 ttatttgtag aaatattacc agccttggca aagccaataa taaacaacac aaataataca
    23641 attgcgataa attttttcat tttaataata tattattttc taaatatttt aattgggcat
    23701 caaagtttcc atatcccaaa tactaggacc atcttttaga ttcaattaag gtgaattagg
    23761 atctgaccaa ggatcatctc caggctctac tgggtcctca ggatccgacc aaggatcatc
    23821 tccaggctct actgggtcct ctggatccga ccaaggatca tctccaggct ctcataactt
    23881 cgtataagat gttactacac caaaagtttg ttttgtagca taggcatctt cagtattagc
    23941 tatagctcca cttgtattta aattagaacc ataatgtttg ttccctttta ctacaactct
    24001 tcgatgtcaa ttttcattaa tattttctaa atcagttttc ccatcaacac caccactttc
    24061 taatttacca tcttcaatct ttattgaaac ttcaattcat tctagattta taccaataga
    24121 ctcattagca cactctcata ataaataaaa cacatattct agatcaagct taattctaac
    24181 tttttgatcc aaaatgtcaa tagattttca taacaacaga ttctttaaat tatcatctgt
    24241 taattccaag ccatcaatat taatttgttt ttgtaatgtc tttttcaata tttctttatg
    24301 tggaattgtc ttatctaaat taccccaaaa ccattcttgt gtagcagata agtctttatc
    24361 ttctattctc tttattcacg agttaggact attctttatt cacaaaacat tggtagctca
    24421 catttgtgtc atagaatatc atcttcattc tttattgttc tgatgataaa aggccgtcat
    24481 cccaaaaaga tttttttctt tccttctttc aagtgtcttt tgtgacctaa aatcgttaaa
    24541 taaactatct ctgtaagtat tttccaacgg aaatctaata tttttattgt atcaatacag
    24601 ctctttataa tgagtccctc ttttagtaaa aaagtatgtc aattgttgtc agttgtgagt
    24661 tttaactcaa ccttcagaaa gcatggtatg ataagcaaac atagacttaa acccatcaac
    24721 tatcataatt ttttcttctg aagtaactgt tccttctatt tttgttctca aattatcata
    24781 aattggatca ctaaaattat tttttttatt tttaagtatt tcctgtaaac taccaaaagc
    24841 tttttcataa ttttcttctt caataacagt tttttccaaa gtagtaaatg attctgccat
    24901 aaattgtcca tatatagacg gaacattatt atccatcctt gaaaggcctt tttctatacc
    24961 atcaaacaat ctatctatct cattagattc taagaaaatg tctatgtttg caaagctttt
    25021 ggtttctctt ttccaactca ttccatcaat catttctata cttgtagcat taccgtctgg
    25081 agttcccaca gtcaattctg ctcctaattg ataatctaac actaaatctt ctgctggatt
    25141 agatttataa tagaaataat acttatcgtt actttgccta atcatggtaa gtgtcccagt
    25201 ttgtggcata attaaactat ctcattctat tgttatcaaa gaattattac caccaacatt
    25261 cacacattgt tttcatatac cttcttccaa ttcaaaaata gcttttcaat tttctacttc
    25321 aaaacattct ttaatcggta aaggatattc tacatatcat tgttctctaa catcattttt
    25381 aagttttatt atttgattat taacatattc tttttctaca ataatttttg cttctacatt
    25441 tcactcatac tttgttggat ctcattgata atcttccata tcatatccca cctgaatatc
    25501 atctaatttt gctttattat ctcatataaa taaatgaaat ctagcactat ttaatctact
    25561 ataatccatc aatcatattt ttgcattagc aggcacaagt aaatatttat tatctactat
    25621 tttagtataa ttaactactt tagaatctac atttatattt gcatacttta ttaaatccaa
    25681 cggaatatat aagacattta cagctcttcc atcagcattt tctgtcattg gctctagttt
    25741 tatatccaaa tcaggagtca aaacatcagg atgggctatt ctcaatttag catttaaata
    25801 atttactcca ttttctgtaa tatatcaatc caaagaaaag ttttctccct ccatctcatt
    25861 cattcatctt cctgtaacta atttttcata atagttagca tgactttgtt ttgtttcttt
    25921 agaatatagg tttttatatt ttgaaaactc tactagtgat ataacaggaa tatatctagc
    25981 caatatttga atagagattc cagcgccact caagtgccat ttagtagcat caagttttgt
    26041 atcatttttc cattgtattg cataggcttc agccacttca tgtacaattg ctgtaagctt
    26101 tggatcattt tctttcaaac ttaatcaagc accataatag gaaattcact tatacaaatt
    26161 ctctgcaact ttagaaatta atttatgctc attatctttt aaatcattca aggatttatt
    26221 ataaaattgc ttagccaagt tctgttttat accagaaata atattttctt tatcttttgt
    26281 taaatccact catgctaatg acttatataa aacaccattt gtaccagtta aagtattctt
    26341 tatttcaaca gattgttcat ctgttaattc cattttattt ctagatcgtc ataagtatca
    26401 tcctccacca aatcaaactt caggaatcca tgttaaattt catcaagtag aaaagtaatg
    26461 tgctgactta gctttcaaag aatttaaatc tacacctgga tttaatctag atgtaattca
    26521 aatggttcca taagccatag gtaaaaataa agacttattt gttgctcaaa ttcatgttcc
    26581 aaaattaaac ctagtatttt ttcaaatttt cgcagaatta ttatatgaaa aaactatacc
    26641 aagtacagga tctttatctt ttgccacatt tcaaacacct aaactcatag taaactgacc
    26701 agcctttcct gcatcataag ttagtcaggt tcaaacagaa gtacttattc ctcagttttg
    26761 caaaactcca gcataaaata tcggttttaa tactgctcta atttctggat catctaccaa
    26821 tcactgttct aataattttt tatattgatc agcaaaagcg ttgtccaatc cttcaatttt
    26881 aaaagttcca ttttctactt gtggtaaaac ataatctata tactcctctt gtgcggtttt
    26941 tccatatcta aatatgtagt ctataggcat aggactattt gccaaagtag tttggaaata
    27001 gccaagtagt catggattgt tttcaaactc ccctaatatc ttctttaatc agccatcctc
    27061 tcatccttct tcttttaact ttttcacatc tgacaaaagt gcaagatctc cattttgttc
    27121 catcaagatc tcacaaaaat ccataagatt ttcccatgga gcctttatct tgtccttatc
    27181 tgatgaaaat tttctatcaa catttacact tttataaaca ttttttactg taagtcacat
    27241 aacatatcac ctatctccaa agtccactct tccatctcta ttataatctc ccaataacat
    27301 cacaaatttc ttttcagttt cattctcata tcggtgtttt tctttatctt catcataatc
    27361 aatatcaact aaattgtcta ttattttcgt actatttata gcagaaacta tatcttgtct
    27421 tcactcaaca actcatgcca agaaatcaaa cacagcatta tagtaacact cttttgaatc
    27481 actagtatct ttttctcaca ttactacgaa attaccattt ccatctttaa catccccttg
    27541 tttacttttt ttatacttat catataattt tgtaaatttt tcatgttttt tactagcatt
    27601 tggatctgta agatatgtag acaaatctat tttatttacc attcttttta attcttttag
    27661 ttctttacta tcagcaaaac tagcgttcat caattcaacg cttcattttc tagtagattt
    27721 cttaaacaga tggtttacag caagtcttct cttatccgga tctataagcc catcagctct
    27781 tcaaattgct tctctttcgt ataaattttt aaataaaact ttgttgttct cttcatcaat
    27841 aacatgaaac ccatctattc acctaaggaa ataattttcc aacaatcaca taaccttttt
    27901 gtaataatct ccacagtttt gatcctctct gtacatctta tataacccaa aaagttcttg
    27961 tttattttta tctttatctt gtgaaggtct aggtgggata gcaagatcat aatctctgga
    28021 tacatcgtaa aacatatttt gaatagaaac atctttgttt ttatatgtat tcaagagaat
    28081 ctttttcaat tttctaagat ttcttctctc caagagagca ggattatttg tattataatc
    28141 aagagtagaa atactcaaat catcatgtgt tcatccccgc ccaaacaaac ctttaattca
    28201 caaaattctt tttaaagcac gcttctctct ctgaacattt tttatttttt cagatcaaga
    28261 tcaaaagtat ctttccgctt tatattgaat ttcttgattt tttgctacct ttattccttt
    28321 tccaaaattt ttatcatcgg ttaaaataag atcctttatt gtaaagtttc ttttatccga
    28381 tttttcatct atcaaatatt tatctattga atcataatta tccagctcta catcaaaatt
    28441 tacaaatctt gttcttgata aatcaatttt ttttctttct gatcccaatc ttttcataaa
    28501 ttctttttga gaaatatact ctccaccaaa ataataatca caattttcat ttaacttaat
    28561 ttctaaaatt tcccaagaat tatttaagta ctctttcata tgtatctctg cctccttcaa
    28621 aactaaggct tttgtaatag cctctatttc attatttcta tcagataatt tttgcacttc
    28681 ttctttctct ccattttttt cttttaagtc ctcaatcttt tttaaattat ctttatattc
    28741 agtctttatt tctctaattc ttttttcaat atcctctttt ttattaccat ttttttctat
    28801 atctttcacc tctttttctg cttttgctag caaactttca tatttattaa tatccacttt
    28861 ccaaatttca tcctcttcta catttactgt catatttttt ctatatatct cttttcagcc
    28921 tccatccttt accactatag taaaattatc ttgtccagta aattttcatt catattgtaa
    28981 tttcccatct aaaagcgtta ctggaatatt tttagcatca tcaaaactat atggaggaat
    29041 tacttcaaat gtagtgccat ttgggacact tgctaaatca accttttcat ccattttaca
    29101 ttcaaaaacc atatcttttt tatcaatttc tactcaagta tctagtcaag atttagtagc
    29161 atcaatatta ttaaccccag aattaccagt accaacattt ggagttttac caatactagt
    29221 accaacaatg tcagcacttc aaaatgaagc agtggtggtt gtcgcatttc ccacactaga
    29281 attttgatcc ccagcacctg ctgatacagg attattgttt atataatttt ttagtgcatc
    29341 aaaagtctct gttcaaaaca atccatcagt tcatggattt ttcatgaaat catttgtttg
    29401 caacttacct ttttcatagt tcaaaccaac aagcatacat tgtagccttc taatagattc
    29461 cacatttcac tcttttataa atcaaataac tgtatcgcga tctttaaaat ttttattcaa
    29521 ttcctcaatc acttcatttt ttctttgatc taatcaacga tatagtttta atgttaaatc
    29581 gccaacacca atcaaatcca aatttacttg tttctcagtt tccatttttg cattaataac
    29641 ttgagtttga aaaatttcta actcaactaa tgttttaaaa ttctgtaatt caaaagttgt
    29701 aggtacaata atctgtttag caatctcttt ttctttaaaa gaatcaagtg taccaaactc
    29761 tttatccaac cagccattaa ataccggctc caacacctct ttcgtagttg ctgtagtaaa
    29821 ttgatttttt ttatcttcca aaaaatcttg aaaagatttt tcaacttctg catcaaaatg
    29881 tttaaaatca aatttatcta ttcattcgat agattttaga ttagaggcca aattgtcttt
    29941 tttttctata tctctaacat aatccttttg gtttgccatg tttgcaataa aataataaaa
    30001 ctttaacaca ctatcaattt tattcaaaaa taaataaatg ccaaaatttt tactatatta
    30061 tatacactta gccaaccata aaacaaaatt tatgatatag tatgtattta taaaaaataa
    30121 ttaatcactc aaaaaatatt ataaaaattt attctttttt ttcttgaaat ttttcatcag
    30181 gattttcttt ttgtccaaaa atacgcacat catttttacc tgtatctgga gattcttcat
    30241 taatttcctt agcatttttc aataactcta taggtcttat tgttttatta tattctacac
    30301 tttttacaga atgtccattt gcaaatccat ccagcaattt ttggtttgga ttttttgatt
    30361 ctagagtacc ttcatttgac tcagattttt tcaattcttt tcatcttaat tttcattcag
    30421 tttttcaatc tttagcaatt tctctaacat tttttataat ttttctcaat cgagcaaaaa
    30481 atcacggttt ctctttttta tctttagtaa gattttcttt ttcaaaacta ctctcttttt
    30541 gttctcaatc tttattctca tctttttcat catttttctt attccttgaa aataattttt
    30601 taaaaaatca tcaatccttt tgttctttct tttccctttc atttttatct tccaacttct
    30661 tttctcactt tccttcttcc aactcttttt cttctcttgc ctccacagat ttctttcttt
    30721 cttttatttc ttcaaacctc ttttcttcct ttacttcttc atgcctttct tcaaatatac
    30781 caaaattact taaaaattct cacacagttc atttcccctg ttttataata tttactttat
    30841 gttcgggatc tatatattgc tctatatttt tatctttttt ttcaagtacc tctatactca
    30901 ttaaaataat atcaataaat aaaaaaatta ctatatatat agtataatca aaaaaaatat
    30961 cctgtcaaat tttaaattgc aatttattgg tcaaattaaa tgttcttgaa aaaacacaca
    31021 aaatcaatat cagaaaatca catatcaaaa aatgtttttt tgaaataaaa ccttgccgcg
    31081 atagttcagc tggtagaaca ttcccatggt aagggaaagg tcacgggttc gatccccgtt
    31141 cgtggcttat tacatcactt ataatgaata atcaacaact aaagactaac aattattgac
    31201 tattgactaa caattattga ctattgacta gcgactattg actaacgact agtgactatt
    31261 gactatcgac tattaccttt ttaactttaa aaactttcaa ctccatgttc tctcctagca
    31321 tagaaatatt aaataaatat gcagatgttt tggtaaattt tgctctacga aacggaaaat
    31381 gaatcaaaaa atgagatgta gtttttgtat atattccaga atcagcaaaa gcattttaca
    31441 ttccacttca agaaacaatt ttaaaggctg gatgacatcc aattatgaaa tatatggctg
    31501 aatgagtggc taaaaatttt tatgaattag catcaaacca gcaattacaa ttcttaccag
    31561 atgcttacaa aaaatgagaa atagaatcta agactcatca tatagatata ttagcagaaa
    31621 tggacaaaca cgaactaaaa tgaattgaac caaaaaaaat tatgatgaga atcaaagcaa
    31681 actatccgta caaagagctt ttaaataaaa aagaaagaga aaaaaacttt tcctggacag
    31741 taggactttt tggaactcca gcaatggcaa aagaagtttg actaagttta aaagcatatc
    31801 gagaccaaat aattcaagcc tgctttttgg ataaaaaaaa tcccgtaaaa aaatggaaag
    31861 aaataaatag ttggatgata aaagaaaaag acaaattaga taaattacaa atagaatact
    31921 tacacataaa aggagaagat gtagatttaa acatcaaaat tggaaaagat agacgctgga
    31981 aagcgggaaa tggaataaac ataccttcat ttgaaatatt tacatctcca gacaaaagag
    32041 aaacaaatgg acgaatcaga tttaatcaac cgttatatag gtatggtagc ttaataaaat
    32101 gaattgaaat agaatttaaa gattgaaaaa tagtaaaagc aagcgccaaa gaaaacgaca
    32161 aactatttca agatatgata aaaattccat gaatggagta tgttggagaa tttagtttaa
    32221 cagatgctag aacttctaga attaccaaat ttatgtgaga aactctttac gatgaaaatg
    32281 ttggatgaaa atattgaaac actcatatag cactttgaaa agcttttgat gaatgttata
    32341 acggtgatat ttctaaacta gacaacccaa aattcaaaga gtccatcggt ttaaacacct
    32401 ctgctgaaca tgtagatata atctccacta caaacagaac tgtaacagca accttacagg
    32461 actgaagcaa aaaagtaata tataaagact gacaatttac gatataagct tgaaatccaa
    32521 gcgacaaaat atatacttgc aacaagcttt attattgtaa aataacttta caagctttta
    32581 actttacaag cttttaactt tacaagcttt taactttata aacttttaac tttcaacttt
    32641 aatgtaatgg caagtaggac aaacaaaaga tgaattgcag ccaaaaaagc acaacaacaa
    32701 aaacaagcac aaagactaaa atgactcaaa agatttgagt atccaaaaag aaagctagaa
    32761 cttgaagaag caaaagaact ttgagtagtt tgagtattta aaaaccctaa atgatcagaa
    32821 tacaaccgat ttataagatg aagaaaaatt tgccgagaaa cgaaacttag agttgatcca
    32881 gatggataca gatacattag aacaccttct ggaggctccg aaatggttca agtaccaatt
    32941 tctggtcggc taagaaccag aatgagaaaa aacatgaact aaaactttct tatttctagg
    33001 aggattaatt ttatcttgaa attaaatctt atttttttat aaggtcgtcc agttgatgtt
    33061 tttgggtcaa gagaaattta taaaacaaat gaggctttct ctcgacctaa aatcacaaaa
    33121 atacaccaaa ttcctatgct gagataattc agaagcttgt cccacagagc atagcgaagt
    33181 acaggagtag agacagtcct gataaatcgg gatgtgtcgg tcacagtaaa attgaagtaa
    33241 agtatcctta catgccgggg tagctcagag gtagagcaga ggcctgaaaa gccttgtgtc
    33301 ggtggttcga ttctgcctcc cggcaaatta aactaaaaaa tattcaactt tagaaactta
    33361 taaacttgat aaacttacag gttttgaggg catatagctc agttggttag agcgcatctc
    33421 tgataaggat gaggtcggtg gttcaagtcc acctatgccc accattgtga caaattatcg
    33481 ttaatttaat tacttagagc gtcccgattt atcgggaagg tcggtggttc aagtccacct
    33541 atgcccacca ttgtgacaaa ttatcgttaa ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33661 nnnnnnnnnn nntaattact tagagcgtcc cgatttatcg ggaaagtcgg tggttcaagt
    33721 ctgtctgccg acaaaggcgg acctgtccac caataatatc tatgtatgcc caccaactaa
    33781 attaaaaaga cagtattcgg ggattagctc agatggctag agtacttggt ttgggaccaa
    33841 ggggtcgcga gttcgagtcc cgcatccccg aaattaaaac aaaaaagtaa tctgctggaa
    33901 agtaaacaaa aaattaaact gcacgataca gtgattccaa cctggaatta attatctgta
    33961 gtaataagtt tcttaccaaa atggtggtac acagccattg agtgttgtat gttcactatc
    34021 agatttggag aaattaggtt tttttttaat ttactcttga aattaaaaac aaaatcacta
    34081 caagtacgca acctaaaaac taaaaaacaa acaaattatt ttatttaatt atatttataa
    34141 ttatggctga taaagttcat gtaaatgttg gtaccattgg tcatgttgat catggtaaaa
    34201 caacactaac agcagctatt actgctgttt ctgctgctaa aggttttgct aaggcgcaaa
    34261 actatgcaga aattgataat gctccggaag aaaaagcaag aggtattact atcaacacaa
    34321 gacatgttga atacgaaact gaaacaagac actacgctca tattgactgt ccaggacatg
    34381 ccgactatgt aaaaaatatg attgttggtg ctgcacaaat ggattgagct attttagttg
    34441 tggctggcac agatggacca atgcctcaaa ctagagaaca cgttcttttg gctaaacaag
    34501 ttaatgttcc aaaaatcgta gtgtttgtaa acaaagctga tatggttgat gatccagata
    34561 tgttggatct agtagaagaa gaaattagag acattcttac tcaaaatggg tttgatggag
    34621 caaacacccc tatcattaga gggtctgctc ttaaagctac agaaaatcct tctgatcctg
    34681 ttgcagcaaa acctatagaa gatcttttgg ctgcactgga ttcttatatt cctgttcctg
    34741 ttagagaagt tgacaaagat ttcttgatgc cagttgaaga tgtgttttct atcaagggta
    34801 gaggaactgt tgctacaggt agaatagaaa gaggttccgt taaagttaac gatgaagtag
    34861 aattgttatg attaggtaag gagccaaaaa ttacagttgt aactggagta gaaatgttcc
    34921 ataaagcttt tgataaagct gatgctggta tgaatgttgg attattatta agatgaatag
    34981 acaaggaaga aatagaaaga ggtcaagtat tatgtaaaaa aggaacagta aaagcgcaca
    35041 acaaatttga agctgaagta tatgtgttga aaaaagaaga aggaggaaga catactccat
    35101 ttatgtcagg atacagacca caattcttct tgagaactac tgatgttaca ggatcaatta
    35161 atttaaagtc tggcacagaa atgattatgc ctggtgataa cgcaaaaata gatgtagagt
    35221 tgatatatcc agtagctatg gatgaaggtt tgagattcgc tatcagagaa ggtggaagaa
    35281 ctgttggtgc tggagttatt acaaaaatca actaatttaa aactttaaaa aattgttctc
    35341 atggcaaaaa aagaaaaaaa agaattaaaa ccaaaattaa gaataaaatt aaaaagttat
    35401 gatgtaaaaa tgctggaatc ttcggtttga aaagttgttg gacttttagt aagatcctga
    35461 gcagaaataa aatgaccaat tcctttacca aaaaaaagga aactttatac tgttttaaga
    35521 tcaaacttta aatttaaaga ttctagagaa caatttgaaa ggatgactta ttctaggatg
    35581 ttggatgtta cagaaatgtg acctaaaaca atggaaaatc tacaaaatct atctatacca
    35641 gttggtgttt tggtagatat aaaagtattt taaatcaaac tcaaaatatt tttaaaatct
    35701 ccaacgcctt ccttttaatt aatgaataaa atttctactt atgaatagag catgactaat
    35761 tgtttccaaa aaagaaatga ccagagtttg gaaaaactga aaaatgatac cagtaaccat
    35821 attaaaagta ttaccacagg aattattaag atacaaaaca gtagagaagg attgatattc
    35881 tgctgctatt gtttgagtaa acaaaaaaga aacaaacaaa gaaaagtgac aaaaagttaa
    35941 atattctata actgttgaat ttaacaatat agatgataat tttttggaaa cttacaaatc
    36001 atgatctatc ttggacatta atttattgga aggcgtaaag gaggttaact tggtttgaac
    36061 ttctaaaggg aaatgatttc aatgagcaat gaaaagattt cacctgaggg gatgaccaaa
    36121 aactcgttga tccaaatttc ataggcaagt atgagcctta tgaaacagaa agcctagaag
    36181 agttcaaaaa tgacgccccc atgcatgaca tatgtgatat gaaaaagtta caattaaatc
    36241 cgtagaaata atagatagaa taatagaaaa taatgaacaa ctattaatat taaagggttc
    36301 agttcccgga tcctacaatt cattcattca aatatttgct taaataagca aacatttatt
    36361 atgtagttat gccaaaaaat gtgatatact atagatatat acgacaaaac atgaaaaaaa
    36421 atctctactt tgaaattaaa taatgagata ttttcagaag ataaaataaa ttataatctg
    36481 atacacgagt attatttgtt acaaacaagt aatgcgcgta atcccatcgc tcacacaaaa
    36541 acaagatgag aagttgcagg ttctggtaaa aaactttaca ggcaaaaagg aactggttat
    36601 gctagagtat gagacagaag atcacctatt agaataggcg gtggtgtggt tttttgacct
    36661 agaaacgaaa gaaattatac aaaatctatg aacaaaaagt ctagacaact agctttaagt
    36721 gggattctga cactaaaagc aaaagataaa aacattatct gattgaaaga ctttgagatg
    36781 aaagagccaa aaacaaaaga agcactaaat attttaacca acatatgatt aaacaaagaa
    36841 aagaccttat ttgttttaaa tgaaaaaaac atcaacctaa taaagtcatt tagaaactta
    36901 aataatgtaa agtatttatt ggctgattac ttaaatccaa aagatttatt ggagtacaat
    36961 aaaatattat ttttagaatc agcattaaac aaaataaata caaaataaaa aaaccatctt
    37021 gcatataatg tacaacttta tactttaaaa actttgattt atgacatata gagaaaaatt
    37081 acaaaggaga gcaaaaaaaa acatggaaac caaggctcta aaaaaactat ctccttatga
    37141 tattatactt tgacctaatg ttacagaaaa gacttataaa ctgcaagaaa caaataatca
    37201 atattttttt aaagttcata aaagtgcaaa caaaaacgat gtaaaacaag ctataaaata
    37261 tatttacaat gtaactccca ttggaataaa cattattaat gtggctttta aatgaaggaa
    37321 tcaaagaaaa ttagttagaa gatctcataa aaaagctata ataacattaa ataaaaaaga
    37381 taaaatagaa ttgtgattat aaaaaactat tagataaaca ataaatattc aactttaaac
    37441 tctataaatt aggtaatggc catcaaaact tataaaccat atactccttc aagaagattc
    37501 atgatatgat acgattttag tgatcttact accaaaaaac cagaaaaatc tttgacgaaa
    37561 tttttaggta aaactggagg aagaaataat aaatgaagaa ttactacgag agctagatga
    37621 ggcggacaca aaagattgta cagattgata gacttcagag cttatgacaa aatagacatt
    37681 ccagcaaagg tagctagtat cgaatatgat ccatatagga cttgtagaat tgctttatta
    37741 aattatgttg atggagaaaa aagatatgtt ttagcacgaa aaggaatttc tgtttgagaa
    37801 actgttatga gttgagaaaa cgcagagata aaagcttgaa acagaaaaag actaaaagac
    37861 attcccgaat gagtaaacat attcaactta gaaataactc ctcaatcaaa aggaaaatta
    37921 gttaaatcag cttgaactta tgctactata tcttgaaaag atgatttaca aggattagtt
    37981 ttcataaaac taccatcagg agaagtcaga aaatttaatg aaaattgtcg agcaacaata
    38041 tgagaggtta gcaacgaaca acacaagaat atagttatat gaaaagctgg tagacaaaga
    38101 cgattaggaa aaaaaccaaa agtattatga gtaaatatga atcctgtaga tcatccacac
    38161 ggatgatgag aggcacatgc agatatctga ttaaaaaaat gaccaaaatc atttacaggg
    38221 aaattagtcg ctccttgaat taaaacaaga aaaggtcaaa aatggtcaga caaatttata
    38281 gtatcaaata gaaaaaagaa ataatcgatt tattcaaaaa atttaaaatt taaattaaaa
    38341 taaataatgg ctagatcact aaaaaaatga ttctatgtaa atgaaaaact tttgataaaa
    38401 atcaaaaaaa tgtcaacaac ttgagacaaa aaagttttaa aagtttggga tagggcttgt
    38461 catattattc ctgatatgat aggacttact tttgctgttc acaactgaaa acaatttgta
    38521 agtgtatata ttacggaaga tatgatatga cacagactat gagagtttgc cccaactaga
    38581 acatttaggg ggcacccttt ttaaagaatt taaataagtt ttaatacatt aatatcctaa
    38641 agttatgagt aatgaaaata tgacagcaac attaaaatat gctatagtat ccgataaaaa
    38701 aatgttacta gtatccaagc tggtaaaatg aaaaaaggta caagaagcct taaatattct
    38761 agggtatata cccaaaaaat cagcaaaaat acttgccaag gtaattcagt ctgcatcatc
    38821 aaatgctgtc gtaaattgaa aaaagaaact gccagattta tttatagaag caatagaaat
    38881 tggcaaatga ccaaaaataa aaagaataag gtttacatca agatctaggg ttagtcatta
    38941 tgaaaaatct agatgttatg ttaaagtcgt tttaaatact aagtaattat cttaatgtga
    39001 caaaaagtaa atcctatatg aatgagagtt gggataatga agtcccgacc atcagaatgg
    39061 tttgctaaga caaagcttca atgagcagat ttttttgttg aagatattaa aatcagaaac
    39121 tttatagaaa aaaaatactc acaaagcggt attgcaaaaa ttattataag aaaaacttca
    39181 aaggaatgtg aagtaataat atttgcaaac aaagtatgag ttttaatgtg aaaacaatga
    39241 gaaaatatca aagacatgga aaatgaatta aataaaaagt ggggtaaaaa attcattgta
    39301 agtgtaaaag aagttagagt tccagaattt tctgcaaaaa taatggccga atttatatca
    39361 aatcaattgg aaaatagaat gccatataga aaggttgcca aaaatgtgtt acaaaaagtc
    39421 atgcaaaaag gagcaaatgg tatcaagata tctattggtg gtagattaaa ttgagctgat
    39481 atagcaagga cagaaaaatt tattgatggt agggtatctt tgcaaacatt tagatctgat
    39541 atcgattacc atgagatgca ggctatgaca aaatattgag ttttaggggt aaaagtttgg
    39601 atagaaaaat gaaccttata caataagaaa aaaactctta aaaaaactat caatttataa
    39661 aatgtaagta cagtgtttat ttaacgaata atttataaaa atgttgttaa ctccaaaaag
    39721 acgaaaacat agaaaacaaa tgatcccttc tttaaaaggt aaaagtacaa gatgaactac
    39781 catcgctttt tgagaatatg gactaaaagc tattaccaac ggatatgttt caaataaaga
    39841 gatagaagcc gctagaaaag tggtagtaag gcacacaaga aaaacttgaa gactacgaat
    39901 tagggttttc cctgatattc cttttacgaa aaaatgatta gaaatgccca tgggtaaagg
    39961 aaaaggagat gtagatgttt ataaagcacc agtaaggaga ggaaaggtta ttttggaagt
    40021 aagttgatta gaaaaaaatt tagcaaaaaa aattcttatc agtgcatcca aaaaactagg
    40081 tatacaaaca agggttgcca tcaaatgaaa aattcaataa aatttattta gttttaatgt
    40141 atataaaatg aaaggtaaag gagcttacat caaacaatta aatgaaaaaa caataagaga
    40201 acttataagt gaaagaaaaa aattaaaaaa agagttatat aatttaaaaa tgaaaaatgc
    40261 tattagaggt ctaaaagaaa cccataaaat ttgagaaatt agagtaaata tagcaagagt
    40321 aaatactgtt ttaaaatcta aaataagtat aaagccatga cagtaatatg agataaaaat
    40381 attaaagaac ttatctgaga agttgttagt gataaaacca ataaaactcg tacagttttg
    40441 gtaaaaagcg ttaaaatgca ccctatttac aagaaaaggt tcgtagtttc aaaaaaatat
    40501 tatgctcacg atgaaaacaa ctcttctaaa atgggagatc ttgtaaagat tagagaatgt
    40561 aaacctctaa gtaaatcaaa aaggtggtat tttatcgaaa tcattaagta atattacatt
    40621 tttatagatt aaactttatt acaaatgtta aagaatgaat ctatggtggt agttgcagac
    40681 aatacatgag ccaaaaaagc acaaataata agaattctaa aatgatcaat ggcaactact
    40741 gcaagtgttt gagactttgt tgttgtagca ataaaaagcg tcattccaaa tagtcatgtt
    40801 aaaaaatgac aaattacaaa ggctatagtt gtaagggtaa aaaaagaagt tcctagaaaa
    40861 gatggtactt atattagatt ttgagacaat gctgtagtat taatgaccaa aaatgaaaaa
    40921 tgagaaataa gtcctatagg taaaagaata ttttgacctg tagcaaggga attaagagat
    40981 ttgtgatata aaaacatcac aaatatggct gaagaggtgc tataatattt ttaatatttt
    41041 aattatcttt gctatgaaaa aattaaaatc ttgagaccca gtaatagtaa tatcatgaaa
    41101 atataaatga aaaatatcta ctatagaaaa aattgactga gattacgcta tagtaaaaaa
    41161 cataaatgtt gttaaaaaag ccatgaaatg aaagggattt ataaaaaaaa cactaccaat
    41221 acatatatca aatattatgt attatttaga aaaagaaaaa aaagcttcta aaataagaat
    41281 agaaatcact agtgattgaa aaaaaattag aaaaactaaa aaatgagata taaaaattaa
    41341 ataagtagtt ttatttgtat tcaacagcaa taaatggcta aagattataa aaagaatctt
    41401 caaaaaagat taaacttaaa aaatattaat gcggtaccaa ccctagaaac agtcatagtt
    41461 aatatgggga tttgatctat tgtcactagg aaatgacata aagattttga agaatttgaa
    41521 aaaaatctgt ctaaaatcac atgacaaaaa cctcgtatag taaaagcaaa aaaatctata
    41581 tcaaacttta aattaaggga atgaatgcct gtaatgcttc aatcaacact tagaggcaat
    41641 agagctttag attttattga caggttatat aaaataatat taccaagaat cagagatttc
    41701 tcatgattga ataaaagaag ttttgattca caagccaacc taacaatagg aataaaaaat
    41761 tatagtatat tccctgagtt gggattagat gatgtaacaa tcccaatgtg aatcggaata
    41821 actatagtaa caagcacaaa taatcaagaa aaatctatag cattattgga agagctagga
    41881 ttcgtgttta aataaaattt atatattaaa accaatattt taaatggcaa gagcagcact
    41941 aaaatcaaaa cagtggaaac tttatgaaca aaagttcatc caaaaaaaag aaactaaatg
    42001 aacaaaatat tacaatagat gtagattatg tggaagatca catggatttt ttagagattt
    42061 tggagtttgt agagtttgtt tgagaaaata tgctagagaa tgaaaaataa tgtgacttaa
    42121 aaaagctagc tggtaaaaat tatcatttta ttttatgaaa aaattaatta tgagttatgt
    42181 aaatgctcct attcatgatc tgttgattag aat
//
LOCUS       DAOSID010000003        31498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_1631_length_31498_cov_15.054957, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000003 DAOSID010000000
VERSION     DAOSID010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 31498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31498
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1631_length_31498_cov_15.054957"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3332
                     /locus_tag="PK674_00415"
     CDS             <1..3332
                     /locus_tag="PK674_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15035.1"
                     /translation="IESKNLKFDLNNINKISHLYIFGKSDLESEVEGEQQSTGVMDKE
                     SLSGLLGLNEMRGTDTGESLARETFEDLENYAYEEDPYYNFDEIKNKYNSLDQDGKNK
                     FEEILEQLSDESDDEPTKNLTKELLDYLHGTEDDNENKEDEETPREHIEETEEEKFMK
                     ERESLAGYDFYGEEDKGFRVGTRLFINTGGSKLPPMDKNDSFFEFEIVSIGSSTFNVK
                     VVGNEIQSDSVGQIFTLPKTAEHLSKIKSSGEVFKVAKIDGNDWDTSIKNIIKSNFFK
                     NLNVFGDKEGQVKLKNGKLVNNKGEEIHSFGRSNAIWDGNKKGRPGQAREHTTYEIKN
                     VNKSKGTVKVACNFEGQDENDLSKFLNYKYENELSFEKFILLMESKGLRAYTKQEYED
                     RVVEYNIDCKKHEKVGYPQKGLLKFYSLAAIGGALKNGFKTIQDGIKKVREDQTEDFE
                     NFLYSQGGLNLYSKLGSLTSIFGIFPTMSEAFDRVQLEFYNERENKTWKKIEIWYKMY
                     ESDPHFSSLRNSQLKDLLTTPGYVGGDRNRHKVAAAFLILMKKDGPYGRGGLLQYLGK
                     GFWVEKFLGKEHRIRFKNMQEERKRQLEEYSGMESVHWRKKKQAELNRLEFDYIVGII
                     DGRQPFLSLSDEYLWAGIWSRKFANTLSEHSNAYFTSLKKRSDEIGEIGFRQAEQEYF
                     RMITRGRISKALPFLKRVMEDAQSKDEQSRAKMYFIGAILTGLIKNGQEDNTMHEFWA
                     IARTMGFLPGIWCRDTDQADKLLILLDGITSKPPFEKTFSRELEYKKSDFELGTFNPK
                     QSSMNFLAKDLPNYWKKYGNKILDILEFRDMTSENSIINLATKGGADSYVYKDIINLS
                     REGTKEDISQKVSYTYGYYTNSPWTANKGVVEKLVPRNGDYKGKENIEIEAAEEFWKS
                     VGRSLETGKQSNRGIVDFYLGKFWNRFDIDARDQGNVNFLMRGLSLVKKLKLEGKHKE
                     AKYNLWYLIKGNLHQKLGTFPSEFGVVIDKFYDFFYNNVELIDGKMARNTFGAEAEIG
                     FNNPYGMLNWNEFYNYVMAMEGISGGGKARSDYQRKHKRNEDNYINWNISGISKYLRQ
                     KNISGQPSVPKSNEEVEYKKA"
     gene            complement(3478..4029)
                     /locus_tag="PK674_00420"
     CDS             complement(3478..4029)
                     /locus_tag="PK674_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15036.1"
                     /translation="MMKKVFLFVSLSLFILFPFALAQIQKVAEVQLTILKYGLVIGTP
                     ANLNLGSVLPGNQVEKTFDDYFWVEDLRGSDIGYYTTIQCDGLYGPDNFVITGIQLLG
                     NSIEFLAGVENQTLLYSNLSSWTDITQPQLYFYKIDNTPLNYHINKYGNKPSIRAFIP
                     NDAPAGDYKGKITYTLYDMSFDL"
     gene            complement(4193..4804)
                     /locus_tag="PK674_00425"
     CDS             complement(4193..4804)
                     /locus_tag="PK674_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15037.1"
                     /translation="MKKIYKLGALIVALFALGFVAFANAQQTNKTVTLIISSGTGNCV
                     YSDYQAIEGQVSMNMSTAVGNPASYRCILGGGSSYVTVQAKSSIKYSSTGIAVPTTGI
                     LMRHIYTGGFNSSYTGELPPLQRDGPYTGGFIGQNPQSKCIVEDTGLNAQFTHINVAR
                     TLFKIHDTRECWYGNETALELRINPNTPVGTYSGDTITFILNN"
     gene            5191..6267
                     /locus_tag="PK674_00430"
     CDS             5191..6267
                     /locus_tag="PK674_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15038.1"
                     /translation="MSIKKPLIGLVIFGLLFTGFSLSEAIFTGGGIEVISESDEETVL
                     TTNVNIASASASLGGIDVRFCNNGLEKLTRKISYVLGQTEKGEICVIVKNNGPKDVKV
                     NLSFLPTSLIEGDGSLSCDHNMENVFGSFMDNSKVQVQVPAGDYIVQNFSIQFPLGIK
                     GKQIGCLFYDVEKEDASAGGIGIIVGKAMFLDYFVLEPGEMLNEETLNEEIINDLELQ
                     IINKEIIDRELIVTVAVRNLGNIDLKTEISGALTNILGIKRDFSLAGEIARANGSFVF
                     SLNFGQLPSYKGLFSIDISAKHTPYFDVSSLEIDPSILEEKETSVSSFYFGTPRIILG
                     MILVIIILLILASKKDENKPLKIK"
     gene            6640..8097
                     /locus_tag="PK674_00435"
     CDS             6640..8097
                     /locus_tag="PK674_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15039.1"
                     /translation="MDRVNHLFRYTNNIQNHFQKHGKKYLFGTLGIFAIMKMVLLFVG
                     FFGTLNLGKSFAQGGEGADPPPTSPVVLDQKNVVSMGCRTDATSCDLSNTGITSIEND
                     TFVHHTQLQILDLSQNQLEYISGVIFPSSLIDVNLSNNQLRNIDGAEFPSSLQRLSLW
                     SNQLKSLYGTHFPHSLQELNLDSNQLANLDGVEFPGSLGFLSLSNNQITNLSGAMFPN
                     SLITLRLDFNQITNLSGIVFSDSLKTLNLSNNEISIIGENIMNLTELYDNGGLNINNN
                     YIDYTQLSPGLLAFIDQKSSTGRRETQTFTPLSPPGGGESSTTLMVTLVVVGGDSACY
                     FSSYPAMEATTSIGGIELRSDPASYYCAINGGSGSYVTVQSSPLISLGHQIPASNVNM
                     RQVLTGGLSSNYTGALPPGAGEELPSEEQKCSLNTQLGTTYTNIASGATLLSTNDLRA
                     CWYGNETQLKIIVPPNTPAGTYSGDIVFIMYDAPQ"
     gene            8271..8531
                     /locus_tag="PK674_00440"
     CDS             8271..8531
                     /locus_tag="PK674_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15040.1"
                     /translation="MDLIVGDFDNLKQNEENEFLLENNIYEFCYALLRARHHFVKGGN
                     IFDGFASFLRKKQKLGDPFLSKIDLNNIYNVAYRGFYIGSGK"
     gene            complement(8598..9686)
                     /locus_tag="PK674_00445"
     CDS             complement(8598..9686)
                     /locus_tag="PK674_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOG15041.1"
                     /translation="MNFNVKMDDKKLKVDIESTNLKDILIYCKPKERLVLTKKFGLNG
                     EKGIPLQRIGKEYNLTRERIRQIETQALMRFRRLIVGNQKYMEVLQEAKRTLDSHGGF
                     LLEDILISKLVNKNMFKFTKQELKLILVSDFDVSFLKRNKYINRSFYLEPLYEDLLTK
                     MTLFIRDYFVTRNSSQDLYEFIGILKENFAKDFREVSYLKNDLFYINFFDAVRDISVF
                     DGKIGLPIFEDVNPKTIKLKILYTMRRINKPIHYQELPAKIVERFPQKPIKLNTVHNE
                     LVKNNDIFVNLGLGIYGLKERGYEGGQVKDILIRIFERNDRAMNVKELCKEMLKEKMV
                     SPNTVMLNLQKHKDLFTRVEKGVYKLKK"
     gene            complement(10320..11426)
                     /gene="ychF"
                     /locus_tag="PK674_00450"
     CDS             complement(10320..11426)
                     /gene="ychF"
                     /locus_tag="PK674_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOG15042.1"
                     /translation="MKIGIVGLPNVGKSTLFNALTNSYSAPAENFPFCTIEPNVGIVD
                     VKDPRIEKLAEISQPKKITHANIEFVDIAGLVRGAGKGEGLGNKFLSNIREVDAIVQV
                     LRYFKDADVVHVEGEVNLMRDIDIINSELIFSDLEKIERILPGIKKKMQTTGDKFLKR
                     EVEILELIQQTLEGNKLANTIVDQLDEQDMKLIRSYGFLTIKPFVYAINVGQDDIPNK
                     DQIQKELQEKLQSPVVIVCAKLESEMMGMEKDEKIQFVSELLEIENIENIPTLDDLIT
                     LAFDTVGLMYYFTTGEKETRAWTIKKGFTAPQAAGVIHTDFEKGFIKAEVVSYVDFVN
                     YGGWNKAKENGKLRLEGKDYVVKDGDIIVFKFNV"
     gene            complement(11474..12085)
                     /locus_tag="PK674_00455"
     CDS             complement(11474..12085)
                     /locus_tag="PK674_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HOG15043.1"
                     /translation="MIYNPYDYYFNKAKQEGYKARSAFKLEEMQNKFKLIDKNTQFVL
                     DIGCAPGSWMQYSYTLLHKFNVQNFKIVGFDILESTVNLPNVHTYVQDVTDLEKVDKI
                     LESNGILPGSVDFIQSDMAPNTVGNKSVDAIRSIGLLEETLWIYQKYLKKNGKFAIKI
                     FMGPGFDEFVATLKEEFGGKNIKIFKPKASRSISKETYILKVS"
     gene            complement(12262..12570)
                     /locus_tag="PK674_00460"
     CDS             complement(12262..12570)
                     /locus_tag="PK674_00460"
                     /inference="COORDINATES: protein motif:HMM:NF039821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF5663 domain-containing protein"
                     /protein_id="HOG15044.1"
                     /translation="MFEDDMFIETLLIKAYPDIEDSALDLLIEDVRPVLYDRVMTNLV
                     QKMPEDKLQEFLDITKKDYSDQELQSFLQKTIKDYDSFIDKVYKDFEDMYLEEQKYVD
                     "
     gene            complement(12636..16841)
                     /locus_tag="PK674_00465"
     CDS             complement(12636..16841)
                     /locus_tag="PK674_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15045.1"
                     /translation="MGTGAQFKSRDFYKSDMNRADELMSRLNKISSRNKVEKQTQQEL
                     LDVFLNSKNSEHIDKVTETIKESREKQGNVNRVNVIDLSSTISFDSSGKIKKVENKEI
                     YDKYNEIAQNSNLNDKEKNQEFEKLDRLLATKIVLLTEQKSILYSFKRRAYFTNEAMR
                     DEVFAELSNHEPFIGNIEKKDYTLDGTMHYFIKWNKKNEITKNPLSKSKKEANLENSK
                     INNININVDTIKLDFVINNFTEIQNKIQDFEIKQSLSILLGKLNSIQKEQGDILIKNI
                     FSESDLNVIAILLAYLDQNFYNDKNKKNVFLSCVQILNKNLGIVNNNSETNVNNKSNA
                     EVNLNLNIGSGGVVPSTIGNYVGGVMPYYQGGISVPLSTGNVNITNQNLVDIITQVLN
                     NINTNNINNNINNNVDAKEKAQDVETIIKTVLEKFVEQMEKSFSEKWSSHKDELVKMY
                     EGIKEHMSKEHREIIEKIINENTEIKEKIERLEKTQKDFSDKLERIEKNHGVIIDKLD
                     DIKNSFEKELNDLKKRQEELHKEIKVLLAKIDGNIGDIQLEIKNIQKEIEELHKKMDE
                     LKKKLDECCEENVVPPIIPVTPDTYEDIATVSNITESYREEASRQAEERLRGRYKRTA
                     WYKPWTRPDKLNLFLRRQHIKEKYERRFMTNLRGVQGDFESSAVADRHEIERQEDFNE
                     AIEVALTDIDENNYPNTRRELDRLILRATGTHPDWIRDNTLSRARFQSELKTILESSL
                     DPNRPATATDPNRRPLSDIYRMNDMDIIGTNIWEKVDNFRDHQILVRNIRDLVIRHRN
                     DLTDDPFINNCRPLISAHISKHRVTPEFLKVNNIKLDDSNSMRQLKILESNNQILTNI
                     AARTLKLKIQLLTRGEEAYNVEQEGGGATRIGEWLDRPYKEDGRLGRYFRRHPAMKEA
                     FSWLWGGTKIAAVAVPAMFLAPFGPLAVASYVGTSTFAQVFFKKHAHYNKEHRGYQTR
                     QATNLAMNTQERQRLNNVVGGMSRFRRNALYYFGLGKTARDVRQFRDYVQTTQNKLED
                     TNQVNASIENLINKNVLLNPEIINLEDRLAEGLARLDFHRETVRGGVSGQNFLGSNTQ
                     ATSESEYQKLHRNILAGASRLGISLDDIRSYTRYNLVRTQIKEGTGQREGTEEIGYDI
                     AVDRKKSRQREKAIRGALKAGGISFGVSYLASTLFSKTVENKNTTTVSGERDPAIHDE
                     SLFVVGDVDAPLHTTLSSNPNIARMDARITAGVDKLRYVDTTKVATEMAAKQASTETY
                     VRTYFTGANRTTLLDAINNTNLGSVQSFAATGGADAGNQYLLATRYIEGVKELAEEMV
                     ATGHTATPLGSISFDIAGSTVEPSVAGSLGNQVARKMGVTLDYFTKKKVALGIGAPVP
                     MNTFGEQLPRGGVNQNRRTRRTR"
     gene            complement(17159..20491)
                     /locus_tag="PK674_00470"
     CDS             complement(17159..20491)
                     /locus_tag="PK674_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15046.1"
                     /translation="MAFRDQNVLNRVNTLNTNLETGNIAEVQRNISNRLFGVLEGANN
                     ISMDSELYASINNVADASDITTSINTAKADLPSTAININYGTPHPVVNSVVTLAAGAP
                     ITDTQNAINAMNTALNGLNALPNQINTLRTRLEGNHRILNDILDIQNIGIPANLNTID
                     LNIRTLNKYKTKCRNLINILNIIDRWENTLGAPPANRNTAVNDFRNAFGQAPSFTVGD
                     LPDGTPFGVTNFDAILDTGSGKLAEYTDLENNIDRLKQQYEEERQYRINLDRLNRQLP
                     SLPSQGIPVPGEFGLSFNANGTIITPIPNTVITTNNAITNTVNRLAELNALEGAIETL
                     RARYQDNLNRLNRILELQNLEDRMNTVYPGEMTRRQTEFSTIRDISHNNLFLGNIGYQ
                     PHLPNQNVNIGGAATNVTLDFVRAPLVGPGRFDYTLCDETGNALRNVGGRFEYQLGGQ
                     TISIGGITFENNNMADQTMTIENLTINPIEGVTFPINLDLNVRVRIHDDTTGLDIDHH
                     KPIHLTIVSPSLGLGAREHAYDTLNPPMNERIEAEYSDYHRVNLENEVIWNILREGGN
                     ENEVNEIYNNETRRNIFIERVRRELAGHFPLLTLANLQAGFRADMTREDRDVPTQYLL
                     GEPEFQNYLRQNVSDNIRNYASTEIRRNVNPQRNLILQTFLTFQADLINNRIDNNDHL
                     DALAAIPNDNPQGHANSAWQRFFRRRSNKNNYTKFFQGREHAVENQSLETETGTINYG
                     VHVEILGVNKIVATINIEGKDEPEIIDAPNHNALIRGILSRAATKDGEPLNRKLRCHL
                     AIGALKALVMMSPQSLRRQIPVTQFQNAQGVNVDCDRIEAGVHGGNLVIRAGGVRDRT
                     RENVTLFNENSFKNLHDVEVLERGIENLSTQINSIMNATAREYETTVNSLRNRSLRGF
                     NTRQFLRFGPVKRLRGRMAYGRTNNDFDFTTTANAGKKSVNISYSRREFTLSGNFEGQ
                     EYEFKSRDIGTLLRRKINRKRVFDGIELEIMEQANEAYIARLRTNSLVATENFAVADL
                     NPDKTGRVYIFDDAGNLSYLEIEDRHLNPIPNGANAGRIPSNRLPVERIRCNEQERKE
                     FMQNPFLSGRLRRVMRRRLALF"
     gene            complement(20612..20896)
                     /locus_tag="PK674_00475"
     CDS             complement(20612..20896)
                     /locus_tag="PK674_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15047.1"
                     /translation="MKTLKEVVNYIHNYMTEYEKGGEVIEKDILYDMMEEELLDTVEG
                     VLTEEDMKNWVNSEDDTFLDKILDERLEDYPGLLESIKNDVLNEYVLGDE"
     gene            complement(20925..21944)
                     /locus_tag="PK674_00480"
     CDS             complement(20925..21944)
                     /locus_tag="PK674_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15048.1"
                     /translation="MSITKAGTSTTTANENKELSLDIFEGVENTTENRKNFLEISSDE
                     LKSLQAKAISDQNYEVAAIIRDVLKEKDSHEQESKNAEFKEELNKAKEEDREQNEKQI
                     ANLKQQLMSGNIDEETLKKTLNELIRSFEENEKRNREANSKLLEDIKNAKNSGNEQEL
                     KRLRGIIKNLALEKKALKDKFAGELPKYDSAEIAKLKTKRVGFKLAGKMKLDGQTVLT
                     GPQLLLRRNIRSRNRINKTVKNLNSIGNDPKKGVNYIMTKASLSEGGRIGSWYKNMKN
                     FFTIRDTNTFDKIFNEHKKKFIDDLESKMDLNSMSDADKKTIAAIKARLDYYQKAYKR
                     QFIVV"
     gene            22106..23446
                     /locus_tag="PK674_00485"
     CDS             22106..23446
                     /locus_tag="PK674_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15049.1"
                     /translation="MAGPNYAGKAVGGVTHVGLTPVQAGISVADAVGTSVKTVLDTGK
                     NLLKTLVYPFSNETWKPKWKEDYKKTWGKEGFSGRQKKHWDNYIAPFKGDGMKAPLLA
                     KATGGITHFATAIPTNAVMTALDATYSAIKTPFSILKNIGKTVIYPFTNETWKPTFGK
                     DLKAIWGKEKGFVARTRDSWINTFDVIPKKGNRGKKKIENDDLEDDFGEEENGAKTIE
                     QSKIIESKNEEGKKEESKNEKNKSHEKVEKEQKDGKQNEQKNEKESKPENKTEEKEKK
                     NENEGKKENEKENANEDGLKKDSIITRKIIKENPDTIFVFGDNVEAKKADAESIAQKG
                     DNEEILAKSGGQAKEMRPAIKDGKKDIENSIGIPTMNGFGKHMTDHEFNENKKVIDDA
                     ISEIKKAAKGKKIHIPFDTKKNQYNIGTGIADLKNKAPKTFKYLQEELKKLESK"
     gene            23492..23941
                     /locus_tag="PK674_00490"
     CDS             23492..23941
                     /locus_tag="PK674_00490"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG15050.1"
                     /translation="MKVSLLTPNLLIIPDINDSDFDFIQKVIASTYLSYYEIQKTQFI
                     VLKDNEEIIAFGRIFPIGENEEELGTLWVKESMRGQKLGITLMKELIKKKKNKPHLYL
                     ACQKELQTYYEYAGFQKINTIPEKLHFTQQWAKENGYSFVVMKYLSA"
     gene            24362..25336
                     /locus_tag="PK674_00495"
     CDS             24362..25336
                     /locus_tag="PK674_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15051.1"
                     /translation="MDIKNFLHQKKENKKKQVMLLSIVLFMVLLISALFFFMVQYYEN
                     ITYNVGDPITVEGKVISDNNFPIYTHSISNSKRSFGLISSTVNLNNFVNKTLIINGEI
                     NELKDNKFPVLQIKNIKIPESKVIIRDNRYFFTNELISFDFSKDLDLSAEKTGRNINI
                     YYQGEPILSMETFVCSKITSTQNCEEMVMNYTKNLNEMFTTYLGYVFYKNKEKTRVSF
                     NDSTMGYIFKTFDNNVLLNVSHLINIINSKFIMENKQDLILNNCINEEKNIRLESISN
                     LSKEIVDENLIKIEVEGLDTDGKLMSCGLIVAILEDRSVKTNKVSYLE"
     gene            complement(25399..25887)
                     /gene="greA"
                     /locus_tag="PK674_00500"
     CDS             complement(25399..25887)
                     /gene="greA"
                     /locus_tag="PK674_00500"
                     /inference="COORDINATES: protein motif:HMM:NF015414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="transcription elongation factor GreA"
                     /protein_id="HOG15052.1"
                     /translation="MATKKVNHITKEGFDKLVTELNHYKQVELPAVHERLSEAKALGD
                     LSENFEYKSAMEDKDFINSKIAELEKLLANVEIIKEEKTKASDIVDYGSKVTLKVEGD
                     DDTYNVTIVGTGEVGIDGDDTLSISLDSPIGHAIKGHKAGDEVKMRLGNDRKSVKIIK
                     VT"
     gene            complement(25912..26232)
                     /locus_tag="PK674_00505"
     CDS             complement(25912..26232)
                     /locus_tag="PK674_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15053.1"
                     /translation="MGKPNVIYKLDGLTEVMTNELKNLVEKNLLKEDSKMSSSLKKIY
                     TKGGDIEVVVKVLIEKVTNGYNGNFRFEFDGEVLEYDRKGFEILSDLVNHAFDNFKQR
                     ILAK"
     gene            complement(26301..26804)
                     /locus_tag="PK674_00510"
     CDS             complement(26301..26804)
                     /locus_tag="PK674_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOG15054.1"
                     /translation="MNKLVKSAGGIIYYYDENNDIRFLLIKRHALSGKIERVAPKGKI
                     QIGETDEKAALREVSEETGIPLNFLSIIQNIGSTTIEGLENKNGKFNKEVVYFLIKYD
                     GDPELVDIQPVEGYLGTHKRATIQEVLNLVYYTDIRELFRKGYVLLRDKQKINDVKKD
                     FLKKLEL"
     gene            complement(26834..27361)
                     /locus_tag="PK674_00515"
     CDS             complement(26834..27361)
                     /locus_tag="PK674_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15055.1"
                     /translation="MVENKDIEFNEGLDDEKNLDEHNEDSDFFDELSSLEDNIRELAG
                     DKIKQKEQVEKEQIVSKTKGNLESLHESMLSGAESRLEQKKISEKGKDKVSEFFDAGQ
                     KEFEKIDTKVEKRVVRRHSQMVDRFAGRPEAVKESIQSSADKILDEIYNWEEEKNPVA
                     RSLLRIVNWIMKTEK"
     gene            complement(27492..28160)
                     /locus_tag="PK674_00520"
     CDS             complement(27492..28160)
                     /locus_tag="PK674_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15056.1"
                     /translation="MKKDDKLLALLDLVIDQYISSGDPVGSKSLNSLGSTKYAPSTLR
                     KYLNLLEKAGFVYQPYKSSGRVPTLDGLSVYIDNYMSGELEQQELDLDNARTGLKYLV
                     ENLGAVADGVVVGFLKNDEYYYLGMNNILRDDLREEYDTTRYIVKLIETRGLVKQLDA
                     KILKKNKVYYTFIQDKNSIVSCIYAKIVVEDYEGMICIVGPIRVDYKKNVAILDKVVS
                     MIGC"
     gene            complement(28480..28956)
                     /locus_tag="PK674_00525"
     CDS             complement(28480..28956)
                     /locus_tag="PK674_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15057.1"
                     /translation="MWEYLSRIFAGLGFMVFIKFCINFLNMYLDALVMTETGIVVYLR
                     EGLLEYKTEVFDRDKIETILHTQKGLRDKLFFRGDITIKLNYGISFPFENIPNPKKQA
                     DKIQHLKAYFSYKKDENSSSVNEEKGVDILVEALGEVVKDYIDKGNKSLDEYGEEF"
     gene            complement(29168..29392)
                     /gene="rpmE"
                     /locus_tag="PK674_00530"
     CDS             complement(29168..29392)
                     /gene="rpmE"
                     /locus_tag="PK674_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L31"
                     /protein_id="HOG15058.1"
                     /translation="MKKGIHSQYYKDVAVQCICGAEFTVNTTVPGPIKIETCYQCHPV
                     FNKDRVVEKMAKGRMEKFLEKQKKIDAMKK"
     gene            complement(29425..29931)
                     /locus_tag="PK674_00535"
     CDS             complement(29425..29931)
                     /locus_tag="PK674_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOG15059.1"
                     /translation="MGTIQQTLVVLKPDAISRSLVGQIISRLERAGLQIVASKMLAPS
                     VEFLRGHYEGIGQLGTRKGEDLLNKVVEYMRKTPVLAMVVEGVEAVEYVRKIVGSTEP
                     KSATPGTIRGDFAHISYTYADSKSGMSVFNLIHASANLQEAELEIKHWFNKDEIFKYE
                     LSYSEFIR"
     gene            30107..30177
                     /locus_tag="PK674_00540"
     tRNA            30107..30177
                     /locus_tag="PK674_00540"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30139..30141,aa:Gln,seq:ttg)
     gene            30311..30385
                     /locus_tag="PK674_00545"
     tRNA            30311..30385
                     /locus_tag="PK674_00545"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30343..30345,aa:Met,seq:cat)
     gene            30430..30500
                     /locus_tag="PK674_00550"
     tRNA            30430..30500
                     /locus_tag="PK674_00550"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30462..30464,aa:Gln,seq:ctg)
     gene            30546..30626
                     /locus_tag="PK674_00555"
     tRNA            30546..30626
                     /locus_tag="PK674_00555"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30579..30581,aa:Leu,seq:tag)
     gene            30698..30770
                     /locus_tag="PK674_00560"
     tRNA            30698..30770
                     /locus_tag="PK674_00560"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30731..30733,aa:Asn,seq:gtt)
     gene            30810..31076
                     /gene="rpsT"
                     /locus_tag="PK674_00565"
     CDS             30810..31076
                     /gene="rpsT"
                     /locus_tag="PK674_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="30S ribosomal protein S20"
                     /protein_id="HOG15060.1"
                     /translation="MPITKAAKKASNRSIKLHQRNSEFKLRLKMAIKKFKKSLERGEK
                     VSPEDLNKIYKYVDKCVKVGVIKKKNGSRKKSNMAKLFSTTQNK"
     gene            31094..31168
                     /locus_tag="PK674_00570"
     tRNA            31094..31168
                     /locus_tag="PK674_00570"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31126..31128,aa:Thr,seq:tgt)
     gene            31232..31308
                     /locus_tag="PK674_00575"
     tRNA            31232..31308
                     /locus_tag="PK674_00575"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31266..31268,aa:Asp,seq:gtc)
     gene            31326..31400
                     /locus_tag="PK674_00580"
     tRNA            31326..31400
                     /locus_tag="PK674_00580"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31359..31361,aa:Glu,seq:ttc)
BASE COUNT        10732 a         4967 c         4467 g        11332 t
ORIGIN      
        1 agatagagtc aaaaaatcta aaatttgatt tgaataatat taataaaata agtcacttat
       61 atattttttg aaaaagtgat ttagagtcag aagttgaagg agaacagcaa tctacttgag
      121 tgatggataa agaaagtttg tcaggattgt tagggttaaa cgaaatgcgt tgaacagata
      181 ctggtgaaag tcttgctaga gagacttttg aagatttaga aaactacgct tatgaagagg
      241 acccttatta taattttgat gaaataaaaa ataaatataa ttcattggat caagattgaa
      301 aaaataaatt tgaagaaatt ttggaacagc tttctgatga atctgatgat gagcctacaa
      361 aaaacttaac aaaagaattg ttagattatt tacactgaac agaagatgac aatgaaaata
      421 aggaagatga ggagacacca agagaacata tagaagaaac agaagaagaa aagtttatga
      481 aagaacgaga gtctttagct tgatacgact tttattgaga agaagacaaa ggattccgtg
      541 ttggtactag actatttatt aatactggag gatcgaaatt accaccaatg gataaaaatg
      601 actccttttt tgagtttgaa atagtgagca tttgatcaag tacttttaat gtaaaagttg
      661 tatgaaatga aatacaatct gattcagtag gacagatttt tacattacca aaaacggcag
      721 aacatttaag taaaataaaa agttctggag aggtttttaa agtagctaaa attgatggaa
      781 atgattggga tacttcgatc aaaaatataa taaaatctaa ttttttcaaa aatcttaatg
      841 tttttggaga caaagaatga caggtaaaat taaaaaattg aaaacttgtt aataataaat
      901 gagaagaaat acattctttt ggtaggtcga atgctatatg ggattgaaat aaaaaaggta
      961 gaccatgaca agcacgggaa catacaacat atgaaattaa aaatgtaaat aaatctaaat
     1021 gaacggttaa agttgcttgt aattttgaat gacaagatga aaatgatttg tctaaatttc
     1081 taaattataa atatgaaaat gaattatcat ttgaaaaatt tatattattg atggaatcaa
     1141 aaggacttcg tgcttataca aaacaagaat atgaagatag ggtagtagag tataatatag
     1201 attgtaaaaa acatgaaaaa gttggttatc ctcaaaaggg tctgcttaag ttttactcct
     1261 tggctgccat aggaggagct ttaaaaaatg gttttaaaac tatacaagat tgaattaaaa
     1321 aagtacgcga agaccaaaca gaagattttg aaaatttttt atattcacaa tgatgactta
     1381 atttatatag taaactgtga tctcttacct ctatctttgg gatatttcca acaatgtcag
     1441 aggcatttga tcgtgtacag ttagaatttt ataatgaaag agaaaataaa acttggaaaa
     1501 aaattgagat ttggtacaaa atgtatgaat cagatcccca tttttctagt ttacgaaaca
     1561 gtcaattgaa agacttactt acaactccat gatatgttgg atgagataga aataggcaca
     1621 aggtggcggc ggcatttttg atactaatga aaaaagactg accttatggt aggggctgac
     1681 ttttgcaata tctgtgaaaa tgattttggg tagaaaaatt tttatgaaaa gaacaccgca
     1741 ttagattcaa aaatatgcaa gaggagagaa aaagacaatt ggaggaatac agcggtatgg
     1801 aaagtgtaca ttggagaaag aaaaaacagg cagaattaaa tagattagaa tttgattata
     1861 tagtatgaat aatagatggt agacaaccat ttttatcgtt gagtgatgag tatttatggg
     1921 ctggaatttg gtctaggaag tttgctaaca cattaagtga gcattctaat gcatatttta
     1981 cgagcctcaa aaaaaggtct gacgaaattt gagaaattgg atttagacaa gcagaacaag
     2041 aatattttag aatgattaca aggggtagaa taagtaaagc actaccattt ttaaaaaggg
     2101 ttatggaaga tgcacaaagt aaagatgaac aatcaagagc aaaaatgtat tttatatgag
     2161 ctatattaac atgattaatt aaaaatggtc aagaagataa tacgatgcac gaattttggg
     2221 ctattgctag aaccatgtga tttttaccat gaatatggtg tagagacacg gatcaggcag
     2281 ataagctact tatattatta gatgggatta catcaaagcc accatttgag aaaacattct
     2341 ctagagaatt agaatacaaa aagtctgatt tcgaattagg aacattcaat ccaaaacaaa
     2401 gcagtatgaa ttttcttgca aaagatttac caaattattg gaaaaaatat tgaaacaaaa
     2461 tattggatat attggagttt agagatatga catcagaaaa tagtataata aatttagcaa
     2521 caaaatgagg agcagactct tatgtttaca aagatataat aaatttaagt agggagggga
     2581 caaaagagga cataagtcaa aaagtttctt atacttattg atattacaca aactctccat
     2641 ggacagctaa taagtgagtt gtagaaaagt tggtacccag aaacggagat tacaaatgaa
     2701 aagaaaatat tgaaatagaa gccgcagaag agttttggaa atctgtttga aggagtttgg
     2761 aaacttgaaa acaaagtaat agatgaatag ttgattttta tttatgaaaa ttttggaatc
     2821 gatttgatat tgatgcacga gatcagtgaa atgtgaattt tttgatgaga tgactttctt
     2881 tggttaaaaa gcttaaatta gaagggaaac ataaagaggc taaatataat ttatggtatc
     2941 taataaaatg aaatttacac caaaaacttt gaaccttccc tagtgaattt ggggtagtaa
     3001 tagataagtt ttatgatttc ttttacaaca atgtggagtt aatagatggt aaaatggcaa
     3061 gaaatacttt cggagcagag gcggagatcg gatttaataa tccatattga atgcttaatt
     3121 ggaatgaatt ttataattat gttatggcta tggagtgaat tagtggaggt tgaaaagcaa
     3181 gaagtgatta tcaaaggaaa cataaaagaa atgaagacaa ctatataaat tggaacataa
     3241 gcggaataag taaatatttg agacaaaaaa atatttctgg tcaacctagt gtacctaaat
     3301 ctaatgagga ggtcgagtat aaaaaagctt aacaggcact ttaaagcaat tctccactct
     3361 gttctttgta ccatttcact attttaacac aaacaaaggt cctttatgaa caaaaaaaat
     3421 actatacaaa aaaatcccga aaaactttcg gaatttttta tttgttagtt taaatggcta
     3481 taaatcaaaa ctcatatcat agagtgtata tgtgattttt catttataat caccagctgg
     3541 tgcatcgttt ggaataaatg cacgaataga tggtttattt caatacttat taatatgata
     3601 gtttaatggt gtattatcta ttttataaaa atagagttgt ggctgagtga tatctgtcca
     3661 agaacttaga ttggaatata gtaatgtttg attttccact catgctagaa attctattga
     3721 gtttcacaac aattgtattc aagtaataac aaagttgtct ggcccataaa gtccatcgca
     3781 ttgaatggta gtataatatc ctatatcgga tcctcttaaa tcctctaccc aaaaataatc
     3841 atcaaaagtt ttttctactt gatttcatgg gagcacactt cctaagttta ggttggcagg
     3901 agttcaaatc actagaccat actttaaaat agttagctga acttctgcta ctttttgaat
     3961 ttgagccaag gcaaaaggga aaagaatgaa caaagacagg gatacaaata aaaaaacttt
     4021 tttcatcatt acaaaaaact ttctaaaatt aaaaaaacaa acaatgtaat aatagttaat
     4081 aatgattgtt gaccaaaaat caaatctttt gtgatattat gctatattat gtggcctagg
     4141 ataaattaat ctaaattatt agattgttat taacctagac ttttgtttgt ttttaattat
     4201 ttagaataaa tgttattgta tctccactat aagttcccac tggtgtattt ggattaattc
     4261 taagttccag tgcagtttca tttcaatacc aacactccct tgtatcatgt attttaaata
     4321 aagttcttgc aacattaata tgagtaaact gagcattcag tcaagtatcc tctacaatac
     4381 atttagattg agggttctga ccgataaagc ctcctgtata gggtccatct ctttgaagag
     4441 gtggcagttc tccagtataa cttgagttaa atcctcatgt ataaatgtgt ctcataagta
     4501 ttcatgtggt tggaacagct attccggtac tactatattt tatggaagat ttagcttgaa
     4561 cagttacata tgaacttcct cctcctaata tacatcggta actagcaggg ttacctaccg
     4621 ccgttgacat attcatggag acctggcctt ctatagcctg gtaatcactg taaacacaat
     4681 tacctgttca actagaaata atcaaagtaa ctgtcttatt tgtttgttga gcatttgcaa
     4741 atgccacaaa accaagtgca aaaagagcca ctataagggc tcataatttg tatatttttt
     4801 tcattttttt atttgtaaca atataaaata tgccctaggc cacataatat aacatagaat
     4861 atttgactat taaagaccgt cttcatctat tttcgccaat acaattataa ttgttttttt
     4921 ttattttgca aatttttggg cacttttttt ataaaaaaaa caaaaaaaag tatatatttg
     4981 catattcttt gataagtccg cttgcttact agaatctctt attgtaccaa aagatagaaa
     5041 aacaaaaaat gtaaatataa ttttttgttc caaaattaaa ttggttgttt ttttgtgtta
     5101 tttaattgat attttgtatc aaaagaatat caactattct agttgctctg atattatcaa
     5161 aaaataattt tatcgttttt aaaaaaagaa atgtctataa aaaaaccttt gatcggtctt
     5221 gtaatttttg gcttgttatt cacaggattt tctctatccg aagctatatt tacatgatga
     5281 gggatagagg ttatttcaga atctgacgaa gaaactgtac taactaccaa tgtaaacata
     5341 gcttcagctt cagcgagttt atgatgaata gatgtgaggt tttgtaataa ttgattagaa
     5401 aaattaacaa gaaaaataag ctatgttttg tgacaaacag aaaaatgaga aatttgtgtg
     5461 atagtaaaaa acaattgacc aaaagatgta aaagtaaatc tctcatttct gcctacaagt
     5521 ttgatcgaag gtgattgatc gctatcatgt gatcataata tggaaaatgt ttttggttct
     5581 tttatggata actcaaaggt tcaagttcaa gtgccagctt gagactatat tgtacaaaat
     5641 tttagtatac aattcccttt atgaataaaa ggaaagcaaa tatgatgtct gttttatgat
     5701 gtagaaaaag aggatgcatc agcggggggg ataggcatta ttgtatgaaa agctatgttt
     5761 ttggattatt ttgtgttgga gcctggagaa atgttaaatg aagaaacact aaatgaagaa
     5821 attataaatg atttggaatt acaaatcatc aacaaagaaa taatagatcg tgaactgata
     5881 gtaactgttg cagttcgtaa cctaggaaat attgatctaa aaacagaaat atctggagcc
     5941 ctgacaaata tccttggaat caaaagggat ttttccctag caggagaaat agcaagagca
     6001 aatggttctt ttgtgttttc cttaaatttt ggacaattac cgagctacaa ggggttgttt
     6061 agtattgata tctcagcaaa acatactccg tattttgatg taagtagttt ggagatagat
     6121 ccgtctatat tagaagaaaa agagacttcc gtttcctctt tttactttgg aactccacga
     6181 attattttgg gtatgatact ggtgattatt atattactta tattagcaag taaaaaagac
     6241 gaaaacaaac ctctcaaaat aaaatagtta aaaaattatt tctgcttggc ttttgtgtta
     6301 cttttttaaa caaaataagg ggctgatctc tgctagagaa ctaggaaagc cctatttttt
     6361 gactgaaaga aatccccgac aaaggagccc tgctggaata cggaagaagc atcgggatag
     6421 ggggtttggg atgattttag gaatcggaga gggagaccaa gagggtgatt caaaaacaaa
     6481 aaaaggattg aaaacttact ttttacaaaa atgcttgatt acataataaa tatatgtata
     6541 ttaagtcaag taaaaaaaat gctaaaatat gttaaattcg ctagccactt tattctaggt
     6601 cttaatttgg ggtgtttttg ttttagtttt tagtaaaaaa tggatagagt taatcattta
     6661 tttagataca caaataatat acaaaaccat ttccaaaaac atggaaagaa gtacttattt
     6721 ggaacactat gaatctttgc tataatgaaa atggtgttac tattcgtatg attttttggt
     6781 acattgaatt tgggtaagag ttttgcacag ggaggagagg gggcagatcc tccaccaaca
     6841 tcaccagtag tactagacca aaaaaatgtt gtatcaatgg gttgtagaac tgatgctact
     6901 tcttgtgatt tatctaatac atgaattact agcatagaaa acgatacctt tgtacatcat
     6961 acacaattac agattttaga tctttctcag aatcaactag aatacataag ctgagtaatt
     7021 ttccctagtt cgttgataga tgtaaatctt tctaataacc aactaagaaa tatagactga
     7081 gcagagtttc ctagttcttt gcagagattg agcctgtggt ctaaccaatt aaaaagtctt
     7141 tactgaacgc actttcccca ttcattacag gaattaaacc ttgattctaa ccaactagca
     7201 aatttagatt gagtggaatt ccctggttct ttgggttttt tatctctttc taataatcaa
     7261 ataacaaatc tatctggagc aatgtttcct aactcattga ttactttacg cctcgatttt
     7321 aatcaaataa caaatttatc tggaattgtg ttttctgatt ctttaaagac tttaaatctt
     7381 tctaataatg aaataagtat tatatgagaa aatattatga atttaacgga attatatgat
     7441 aatggatgat taaatattaa caataactat atagattata ctcaactatc tccagggctt
     7501 ttggccttta tagatcaaaa atcaagtaca ggtcggagag agactcaaac ttttactcca
     7561 ctttcacctc ctggatgatg agaatcatct actactctga tggtcacact agtagttgta
     7621 tgatgagatt ctgcttgtta tttttctagt tatcccgcaa tggaagccac taccagtata
     7681 ggtggtatag aattgcggtc tgatcctgct agttattatt gtgctataaa tggagggtct
     7741 gggtcatatg taactgttca atcatctcct ttaattagcc taggacacca aatccctgct
     7801 tccaatgtaa atatgaggca agttttgact ggtggattga gttctaatta tactggggca
     7861 cttcctccag gagcatgaga agagcttccc tcagaagaac agaaatgtag tctaaatact
     7921 caattgggga ctacttatac aaacattgca agtggagcaa ccttattgag tacaaatgat
     7981 ctgagagcat gttggtattg aaatgaaaca caattaaaaa tcatagtccc tccaaataca
     8041 ccagcaggaa cttatagtgg agatatagtg tttattatgt atgatgcgcc tcaataattt
     8101 aatagaaaaa taaagtaatt agtattacta aacggttaaa acaaaataac taaaaggttt
     8161 tttattgatc aaatttgtaa ctttaggcat atgacactat taaaaaacta actattaata
     8221 atcgagatgt cttttttgat gcatatatgt tcaaatataa ggacagtaaa atggatctta
     8281 ttgtttgaga tttcgataat ctgaaacaaa atgaggaaaa tgaattctta ttagaaaaca
     8341 atatatatga gttttgttat gcattgctta gagctagaca ccactttgtt aaatgaggca
     8401 atatttttga cggatttgct agttttttaa gaaaaaaaca aaagctttga gatccttttt
     8461 tgtctaaaat tgatttaaac aatatatata atgtagcata tcgcggtttt tatatatgat
     8521 caggtaagta aaaaacacag attatccaat ctgtgttttt tattatgtct agggcaatat
     8581 tttttataat atgtttatta ttttttcaat ttgtataccc ccttttctac tctagtaaac
     8641 aaatctttgt gcttttgtag atttaacatc actgtatttg ggcttaccat tttttctttt
     8701 aacatttctt tgcacaattc ttttacattc atagctctat catttctttc aaaaattcta
     8761 attagtatat cttttacttg acctcactca tatcatcgct cttttagtcc atatattcct
     8821 aatcataaat taacaaagat atcattattt ttgactaatt cattgtgaac tgtatttaat
     8881 tttattggtt tttgtgggaa tcgttcaact atttttgcag gcaattcttg gtagtgaatg
     8941 ggtttattga ttcttctcat agtataaaga attttcaatt ttatagtctt aggatttaca
     9001 tcttcaaata ttggtaatca tatttttcca tcaaatactg atatgtccct aacagcatca
     9061 aaaaaattta tataaaacaa atcgtttttt agataagaaa cttccctaaa atcttttgca
     9121 aaattttcct ttaaaattca tataaattca tataaatctt gactactatt tcttgtgaca
     9181 aaataatctc ttataaataa agtcatcttg gtcaaaagat cttcatacaa aggctctaaa
     9241 taaaacgatc tatttatata cttatttctc tttaaaaagg aaacatcaaa atctgatacc
     9301 aatataagtt ttaactcctg ttttgtaaac ttaaacatat ttttatttac aagcttggat
     9361 attagtatgt cttcaagcaa aaaacctccg tgagaatcta atgttctttt tgcttcttgc
     9421 aatacttcca tatatttttg gtttccaact atcaatctcc taaatctcat caaagcttga
     9481 gtttcaattt gtctaatcct ctccctagtt aaattgtact cttttcatat tctttgtaaa
     9541 ggtattcact tctctcaatt taaaccaaat tttttggtca gaactaatct ttcttttggt
     9601 ttacaataaa ttaagatgtc ctttaagttt gtagactcta tatctacctt taattttttg
     9661 tcgtccatct ttacattgaa gttcattttt tggaacatta tattataaaa aatagctgta
     9721 cagtaatata tatagtcaat tgaaattcaa tgtcaagtaa ttttacaaaa ataataaggt
     9781 tcatttatcc taccacccgg tgctgttcgt tgtgctagcc aataataaaa aaaatgtttt
     9841 ttactgtaat aacgggcggt tgataaccgc ccctacatcg ttttatcact ttgtcatttt
     9901 atcgttagtt cagacaaagt tattttttct accatttctt gcagccccgc aattctgcgg
     9961 ggctacatca atttgttact tcaatatttt atcattcact ccgctaagcg gggcaagcat
    10021 ttatcatctc aacactttat cactttatca ttttaacatt ttatcattca ccccgcttag
    10081 cggggcaagt tttatcactt tatcgcgaac tacggtgcgg ggtagaggga tttaacatcc
    10141 tccctaacat tgcaggcgcg accccgctcg gtcctgtaag tcccgcaccg tagtcctacg
    10201 gtgcgggata ggactacggg gcgcgcctac attgtttttg ttttatcatt ttatcatgag
    10261 agaagcgaat atttatcact ttaacatttt atcattttat cacgaacgaa gttcgcctct
    10321 tatacattaa acttaaaaac aataatatct caatccttta caacataatc ctttcattcc
    10381 aacctaagtt ttcaattttc ttttgcctta ttccatcatc cataattaac aaaatcaaca
    10441 taagaaacca cctcagcttt gataaatcat ttttcaaaat ctgtatgaat cactccggct
    10501 gcttgaggtg ctgtaaatca ctttttgata gtccatgctc tagtttcttt ttctccagta
    10561 gtaaaatagt acataagccc aaccgtatca aaagcgagtg tgatcaagtc atctaatgtt
    10621 ggtatgtttt caatattttc tatttccagc aactcggaaa caaattgtat cttttcatct
    10681 ttctccatgc ccatcatttc tgattctagt tttgcacata caatcactac tggagattga
    10741 agtttttcct gaagttcttt ttgaatttga tctttatttg ggatgtcatc ttgtcagaca
    10801 tttatagcat agacaaaagg tttgatagtt aaaaatcaat aagatctaat tagtttcata
    10861 tcttgttcat ctaattgatc tacaatagta tttgctaact tattcccctc taatgtttgt
    10921 tgtattaatt ctaaaatttc aacttctctt ttcaaaaatt tatctcatgt ggtctgcatc
    10981 tttttcttta ttcaaggcaa aattctctca attttttcca aatcagaaaa aattagttca
    11041 ctatttatga tatctatatc cctcattaaa tttacttctc attctacatg aacaacatca
    11101 gcatctttga aataacgaag cacctgaact atcgcatcca cttcacgaat attagataaa
    11161 aatttatttc ccaatcattc tcattttcat gctcaacgaa ctagacctgc aatatctaca
    11221 aactctatat tggcatgtgt tatttttttt ggttgagata tttcagctag tttttctatt
    11281 cttggatctt ttacatctac aatccctaca tttggctcta tcgtacaaaa agggaaattt
    11341 tctgcaggcg cagaataaga gtttgtcaaa gcattgaata atgtagactt tcacacatta
    11401 ggtaatcaaa caattcaaat tttcataatt aatattataa aatataaatt tgggtgaacg
    11461 aacggaaaag tatttaacta acttttagaa tatatgtttc tttggagata ctacgactag
    11521 cctttggttt gaatatttta atattttttc aaccaaactc ctctttcaaa gtagcaacaa
    11581 actcatcaaa tcacggtcac ataaaaattt ttatagcaaa ttttcaattt tttttgagat
    11641 atttttgata tatccacaaa gtttcttcta gcaatcaaat agaacgaatt gcgtctacag
    11701 atttattccc aacagtattg ggagccatat cagattgaat aaagtctaca cttcatggta
    11761 aaatcccatt cgattccaat attttatcta ccttttcaag atcagtaaca tcctgtacat
    11821 aagtatgaac attaggcaaa ttaactgtac tttccaatat atcaaatcaa acaatcttga
    11881 aattttgtac attaaatttg tgtagtaatg tatatgaata ttgcatccag cttcaaggcg
    11941 cacatccaat atccaaaaca aattgagtat ttttatcaat cagtttaaat ttattttgca
    12001 tttcttctag cttaaaagca ctacgagctt tgtatcactc ttgttttgct ttgttaaagt
    12061 aataatcgta tggattgtaa atcatagagt ttagttgaaa gtttgtaaag tttgaaagtt
    12121 cgtaaagtcc atccaaaata ttgcaaactt cttaaaagtt atcaaattaa cgacacaata
    12181 tacataacga tttgttagaa aaaatccact tggaatcaaa aaaaacccaa attaatttgg
    12241 ggttttatgg aaagtttgat attaatctac atacttctgt tcttccaagt acatatcttc
    12301 aaaatcctta taaactttat caataaaact atcataatct tttatggttt tttgcaaaaa
    12361 agattgtaat tcttgatcag aataatcttt ttttgtaata tccaaaaatt cctgtaactt
    12421 atcttctggc attttttgca ccaaattagt cattactcga tcatacaaaa caggtctaac
    12481 atcttctatg agcaaatcca aagcactatc ctctatgtca ggataagctt ttataagcaa
    12541 tgtttctata aacatatcat cttcaaacat tttattttta ttatgaatat aaataattca
    12601 cttatattat tttatctata gattacaata aaagtttatc ttgttcttct tgttcttcta
    12661 ttttgattta ctcaacctct aggtagttgt tcaccaaatg tattcatagg aacaggtgct
    12721 caaattcaaa gagctacttt cttctttgta aaataatcta aagttactcc catttttcta
    12781 gcgacttgat tacctaaact tcaagctaca gaaggctcta ctgtcgatca tgcaatatcg
    12841 aaacttatag atcacaatgg agttgcagta tgtcccgttg ctaccatttc ttctgctaat
    12901 tctttcactc attcaatata ccttgtagct aaaaggtatt gatttcatgc atctgctcat
    12961 ccagttgctg caaatgattg tacagaaccc aagttagtgt tgttgatcgc atccaataat
    13021 gttgttctat ttgctcaagt gaaatatgtg cgaacatatg tttccgtaga agcttgtttt
    13081 gccgccattt cagttgcaac ttttgttgta tctacataac gtaacttatc tactcaagca
    13141 gttattcttg catccatcct agcaatattt ggattggaag acaaggtagt gtgtagtggt
    13201 gcatctacat ctcaaaccac aaataaggat tcatcatgta tagctggatc tcgctctcaa
    13261 gatacagtgg ttgtgttttt gttttcaaca gttttgctga ataatgttga tgctaaataa
    13321 gaaactccaa acgatattca tccagcttta agggctcatc gtatagcttt ttctctttga
    13381 cgagattttt ttctatctac agctatatca taaccaattt cttctgttca ttctctctgt
    13441 catgtacctt cttttatttg tgttctaacc aaattatatc ttgtataact ccttatatca
    13501 tccaaactta ttcacaacct agaggctcca gccaaaatat ttctatgaag tttttgatat
    13561 tcgctttcag aagttgcttg tgtgtttgat cacaaaaagt tttgtccaga tactcatcat
    13621 ctaactgttt ctcgatgaaa atctaaacgc gctagtcatt ctgccaatct atcctctaaa
    13681 ttgataattt ctggattcaa aagaacattt ttattaatca aattttctat actagcatta
    13741 acttgatttg tatcttcaag tttattttgt gttgtttgta cataatctct aaattgtcta
    13801 acatctctag ctgtttttcc taatcaaaaa tagtacagtg catttcttct aaatcgactc
    13861 attcatcaaa caacattatt taatctttga cgctcttggg tgttcatagc taaatttgtc
    13921 gcttgtctag tttgatatca cctatgttct ttgttataat gggcatgttt tttgaaaaat
    13981 acttgtgcaa aagttgaagt tcacacataa ctagctactg ctaatggtca aaatggtgcc
    14041 aaaaacattg caggtacagc tactgcagct atttttgttc atcaccacaa ccacgaaaat
    14101 gcctctttca ttgcaggatg tctcctaaaa tatcttcata gtcttccatc ttccttatat
    14161 ggtctatcca accactcacc tatccttgtt gctcatcctc attcttgttc tacattatag
    14221 gcctcttctc atcttgtaag aagctgtatt ttaagtttca aggttctagc agcaatgtta
    14281 gtcaatattt gattgtttga ctctaatatt tttagctgtc tcatagagtt gctatcatcc
    14341 agtttaatgt tatttacttt cagaaattct ggcgtaactc tatgcttaga tatatgtgca
    14401 ctaataagtg gtctacaatt gtttataaaa ggatcatctg taagatcatt acgatgtcta
    14461 ataactaaat cacgaatatt tcttactaaa atttgatgat ctctaaaatt atctaccttt
    14521 tcccaaatat tagttcatat gatgtccata tcattcatcc tatatatgtc acttaaaggt
    14581 cttcgattag gatctgttgc tgtagctggt ctatttggat ctaaactaga ttccaaaatt
    14641 gtttttaatt cactttgaaa tctcgctcta cttaaggtat tatctctaat ccaatctgga
    14701 tgagttcatg ttgctcttaa aattaatcta tctaattctc tacgcgtatt tggataattg
    14761 ttttcatcaa tatctgtcag ggcaacttca atagcttcat tgaaatcctc ttgtctttca
    14821 atctcatgac gatcagctac tgcagaagac tcaaaatctc attgtactca tctcaaattt
    14881 gtcataaacc ttctttcata cttttctttt atatgttgtc ttcttaaaaa gagatttaat
    14941 ttatctggtc gtgtccaagg cttataccac gctgttcttt tgtatcttca tctaagcctt
    15001 tcttctgctt gacgactggc ttcttcacga taagattctg taatgtttga aactgttgca
    15061 atatcttcat aagtatctgg tgttactgga attataggtg gcacaacatt ttcttcacaa
    15121 cactcatcta gtttcttttt tagctcgtcc atttttttat gcaactcctc aatttctttt
    15181 tgaatatttt taatttctaa ttgtatatca ccaatatttc aatctatctt tgccaacaaa
    15241 acctttattt ctttatgtaa ttcttcttgt ctttttttta gatcatttaa ttctttttca
    15301 aaagaattct ttatatcatc taatttatca ataatcactc aatgattttt ttcaattctt
    15361 tccaatttat cagaaaaatc tttttgagtc ttttctaatc tttctatttt ttcttttatt
    15421 tcagtatttt cattaattat tttttctatt atttccctgt gctctttact catatgttct
    15481 tttataccct catacatttt aaccaactcg tctttatgag aactccactt ttctgaaaaa
    15541 cttttttcca tttgttcaac aaatttctcc aaaactgttt ttattattgt ctccacatct
    15601 tgagcctttt ctttcgcatc tacattatta tttatattat tgtttatatt attagtattt
    15661 atattattca aaacctgtgt tataatatcc actaaatttt gattggtaat attaacatta
    15721 cctgtagata atggcacaga aatacctcat tgataataag gcataacacc accaacataa
    15781 tttcaaattg tagatggtac aactcatcct gatcaaatat ttaaattcaa attaacctct
    15841 gcatttgatt tattattaac attagtttca gaattattgt ttactatacc taaatttttg
    15901 ttcaatattt gaacacaact caaaaagaca ttctttttat ttttgtcatt atagaaattt
    15961 tggtctaaat atgctaataa aatagcaatc acattcaaat cagactcact aaatatgttt
    16021 tttataagaa tatctcattg ttctttttgt atactattca attttcaaag gagtattgac
    16081 aaagattgct ttatttcaaa gtcctgaatt ttattttgaa tttcagtaaa attatttata
    16141 acaaaatcca attttatcgt atcaacattt atattaatat tattaatttt tgaattttct
    16201 aaattagctt cctttttaga ttttgataaa ggattttttg taatttcatt ttttttgttc
    16261 cattttataa aataatgcat tgttccatcc aaagtataat ctttcttttc tatatttcag
    16321 atgaagggtt catgattact taattccgca aaaacctcat cacgcatagc ttcattggta
    16381 aaataagctc ttctcttaaa ggaatataaa attgattttt gttcggtcaa taatactatt
    16441 tttgtagcta acaaacgatc taatttctca aattcttgat ttttttcttt atcatttaaa
    16501 ttactatttt gtgcaatttc attatattta tcataaattt ctttattttc tactttttta
    16561 atttttcaac tagaatcaaa actaattgtg ctcgacaaat caataacatt aactctattt
    16621 acattacctt gtttctctct actttctttt attgtttctg ttactttatc tatgtgttct
    16681 gaattctttg aatttagaaa aacatctaag agttcttgtt gtgtttgttt ttcaacttta
    16741 tttctactag aaatcttgtt aagtctagac ataagttcgt cagcacgatt catgtcactc
    16801 ttataaaaat ctctagattt aaattgtgct caagtaccca tttttaataa ataaattata
    16861 aaaaagtttt ttgactacct aattataact aaaaaagtct tttctgtcaa atttttgagg
    16921 acttttttgc aaaatagttg ggaaaaacaa cgcaaaagta ggtcacaagc gcgattagaa
    16981 actcacacat tctatataaa aatagccaaa tactgaaaaa ttaatataaa aataactaca
    17041 atatttattt gaatataatg ccgaacaaaa aatactaaaa attatttttt tgaacttcct
    17101 acattataaa aaaatccgac cattaagtcg gatttgccaa tattgttaaa gttattttct
    17161 aaaataaagc taatcttctt ctcataactc ttctcaatct tcacgacaaa aatggatttt
    17221 gcataaattc ttttctttct tgttcattac atctaattct ttccactggt aatctattac
    17281 ttggtatgcg tccagcattt gctcaatttg gtattggatt taggtgtcta tcttcaatct
    17341 ctaaataaga caaatttcat gcatcatcaa aaatatatac ccttcatgtt ttatctggat
    17401 taagatccgc aaccgcaaaa ttctcagttg caaccaaact attagttctt aaccttgcga
    17461 tatatgcttc attagcctgc tccattattt ccaattcaat tcaatcaaac accctttttc
    17521 tatttatttt tcttctcaac aatgtaccaa tatctcttga tttaaattca tattcttgtc
    17581 attcaaaatt tcatgaaaga gtaaattctc tccttgaata agatatgttt actgattttt
    17641 tacctgcatt agcagttgtt gtaaaatcaa aatcattatt agttcttcaa tacgccattc
    17701 ttcatcgcaa tctctttact ggtcaaaatc tcaaaaactg tcttgtatta aagcctctca
    17761 aagatctatt tctgagacta tttactgtcg tttcatattc tctagcagta gcattcataa
    17821 tagaattgat ttgagtggat aaattttcta ttcctctctc caaaacctct acatcgtgta
    17881 agtttttaaa tgaattttca ttaaaaagag taacattttc tctagttcta tctcttactc
    17941 cgccagcacg aataaccaaa tttcatcaat gtactcacgc ttcaatccta tcacaatcaa
    18001 cattgactca ttgagcattt tgaaactgag ttaccggtat ttgtctacgc aaactttgtg
    18061 gggacatcat taccaaagcc ttcaaagcac ctatggcaag atgacacctc aattttctat
    18121 ttagcggttc tccatctttc gttgcagctc tagataatat tcatcttatc aaagcattat
    18181 gatttggtgc atcaataatc tctggttcat ctttaccttc aatatttatg gtagccacaa
    18241 ttttatttac tcataatatc tctacatgaa caccataatt tattgttcag gtttctgttt
    18301 ccaatgactg attctctact gcatgttctc ttcattgaaa aaattttgta taattatttt
    18361 tatttgatct acgtctaaaa aatctttgcc atgcactatt tgcatgtcac tgcggattat
    18421 cgtttggtat cgctgctaaa gcgtccaaat gatcattatt atcaatccta ttatttatta
    18481 aatccgcttg aaaagtcaaa aaagtttgta aaattaaatt acgctgagga ttaacatttc
    18541 tacgaatttc agttgaagca tagtttctta tgttatcaga tacgttttgt ctaagataat
    18601 tttggaattc tggttctccc aacaaatatt gagttggcac atctctatct tctcttgtca
    18661 tatccgctct aaatcatgcc tgtaagttag caagtgttaa aagaggaaaa tgtcatgcta
    18721 attcccttct cactctctca ataaatatat ttctccttgt ttcattatta tatatttcat
    18781 ttacttcatt ttcatttcat cattcacgta aaatattcca tattacttca ttttccaagt
    18841 taaccctatg ataatcacta tattcagctt ctattctttc attcattggc ggatttaaag
    18901 tatcataagc atgttcacgt gctcataatc atagacttgg actaacaatt gttaaatgaa
    18961 ttggcttatg atggtctata tcaagtcatg tagtatcatc atgtattcta acccttacat
    19021 tcaaatctaa atttatagga aaagtcactc attcaatcgg attgatagta agattttcaa
    19081 ttgtcatagt ttgatctgcc atattattat tttcaaaagt tattcctcca atagatattg
    19141 tttgtcatca caactgatat tcaaatcttc atcaaacatt tctcaaagca tttcaagttt
    19201 catcacataa agtataatca aaacgccccg gtcctactaa aggagcacga acaaaatcta
    19261 aggttacatt tgttgcagct catcatatat ttacattctg atttggaaga tgtggttgat
    19321 atcaaatatt tcataagaat aagttgttgt gacttatatc acgaattgta ctaaattcag
    19381 tctgtctcct agtcatttct ccaggataaa ccgtattcat tctatcttcg agattttgta
    19441 attctagaat tctatttagt ctatttaagt tatcttggta tcttgcacgg agagtttcta
    19501 ttgctccttc taaagcattt agctctgcta gtctattgac tgtattcgtt attgcattat
    19561 tagttgttat aactgtattt ggtattggcg tgattatagt accatttgca ttaaaagata
    19621 gtcaaaattc tcatggaaca gggatgcctt gtgatggtaa gcttggtaat tgtctgttta
    19681 gtctatctaa atttattcta tattgtcttt cttcctcata ttgttgttta agacggtcta
    19741 tattattctc taagtccgta tattcagcga gctttcaact tcaagtatct agaatagcat
    19801 caaaatttgt tactcaaaaa ggagttcaat ctggcagatc tcatacagta aaggatggag
    19861 cttgtcaaaa tgcatttcta aaatcattaa cagcagtatt tctgtttgct ggtggtgctc
    19921 aaagtgtatt ttcccatcta tctattatat ttagaatgtt tattaaattt ctacatttag
    19981 ttttatactt gtttaaagtt cttatattaa gatctatagt atttaagtta gcgggtattc
    20041 atatattttg tatatcaaga atatcattta atattctatg attcccttcc aatctggttc
    20101 ttaaagtatt gatttgattt ggcagtgcat tgagtcaatt aagagcggta ttcatagcat
    20161 tgatagcatt ttgtgtatct gtaattggag ctccagcagc caaggtaacg acactattta
    20221 caacaggatg tggggtacca taattaatat ttatagcagt ggaaggtaaa tcagcttttg
    20281 ctgtattaat agaagtcgta atatctgatg catctgccac attattgata ctggcatata
    20341 attctgagtc catagaaata ttgttggctc attcaagcac accaaaaagt ctattcgaaa
    20401 tattcctttg aacttccgca atattaccag tttctagatt agtatttaat gtattaactc
    20461 tattcaatac attctgatct ctaaatgcca tctcaattat aataaaataa aactactttc
    20521 tcaatatacc gattgcattt ctctgattaa tctgagtcaa atcatcggta ttttttcgac
    20581 gataataatt gttcatctta tttcataatt attattcgtc tcaaagaaca tactcattca
    20641 agacatcatt tttgatactt tccaaaagcc ctggataatc ttccagtctt tcatctaata
    20701 ttttatccaa aaaagtatca tcctcactat tcacccaatt tttcatgtcc tcttctgtca
    20761 aaactcattc tacagtatcc aacaactctt cttccatcat atcatataaa atatcctttt
    20821 ctattacctc tcatcatttt tcgtattctg tcatgtaatt atgaatataa tttactactt
    20881 cttttaatgt tttcattttt tattaatttt aataaataaa ttttttaaac tacaataaat
    20941 tgtctcttgt atgctttttg ataataatcc aatctagctt ttattgcagc tattgttttt
    21001 ttatctgcat cactcatgct attgagatcc attttagatt ctagatcatc tataaatttc
    21061 tttttgtgtt cattaaatat tttatcaaaa gtatttgtat ctctgattgt aaaaaaattt
    21121 ttcatatttt tataccaaga tcatatcctt catcattccg aaagtgatgc ttttgtcata
    21181 atataattca ctcatttctt tggatcgttt catattgaat ttaaattttt aacggttttg
    21241 tttattctgt tacgagaacg aatatttctt ctcagtaata gctgtggtca tgttaatacg
    21301 gtttgaccat ctaatttcat ttttcatgca agcttgaatc caactcgttt agtctttagt
    21361 ttagctattt ctgcggaatc atatttcggc aattctccag caaatttatc cttaagtgct
    21421 tttttttcta aagctaaatt ttttataatt catctaagtc tcttcaactc ttgttcattt
    21481 caagaatttt ttgcattttt aatatcttct aataatttag aatttgcctc tctatttctt
    21541 ttttcatttt cttcaaaaga tcttatcaat tcatttaaag ttttcttcaa agtctcttca
    21601 tctatatttc agctcataag ttgttgtttt aaattcgcaa tctgtttttc attttgttct
    21661 ctatcttctt cctttgcttt gtttaactcc tccttaaact cagcattttt agactcttgt
    21721 tcatgtgaat ccttctcttt caaaacatct ctaataatag ctgctacttc gtaattttga
    21781 tcagatatag cttttgcttg taacgacttc aattcatcac tcgagatctc aagaaaattt
    21841 tttctatttt cagtagtgtt ctctactcat tcaaaaatat ctaatgaaag ctctttgttc
    21901 tcattagctg ttgttgtaga tgttcatgct ttagttatac tcattttaat ttgtaaaaaa
    21961 taaaatttaa actaatacta gtataagaca aaatataaaa ataatcaaat tattgaaaat
    22021 ttaagtgcat ttagatttga caaaacataa aatttatata taatgatagt gtgtggatta
    22081 attttattcc aaaaaaaata caaaaatggc tggaccaaat tatgcatgaa aagctgtatg
    22141 atgagtaact catgttgggc ttactcctgt acaagcttga ataagtgtag ctgatgctgt
    22201 atgaactagc gtaaaaacag ttttggatac atgaaaaaat ttactaaaaa cacttgtgta
    22261 tccgttttca aatgaaacat ggaaaccaaa atggaaagaa gattacaaaa agacttggtg
    22321 aaaggagtga ttttctggta gacaaaaaaa acattgggac aattatattg ccccatttaa
    22381 atgagatgga atgaaagctc ctttgttagc caaagctact tgaggtatta ctcattttgc
    22441 tactgcaata cctacgaatg ctgttatgac tgctttggat gctacatatt cggctattaa
    22501 gacaccattt tctatactaa aaaacatatg aaaaaccgta atatatccat ttacaaatga
    22561 aacttggaag cctacatttg gaaaagatct caaagccatt tggtgaaagg aaaaatgatt
    22621 tgtggctaga actagagata gttggataaa cacatttgat gttattccca aaaaaggaaa
    22681 tagatgaaaa aagaaaatag aaaatgatga tttggaagat gatttttgag aagaggaaaa
    22741 tggagcgaaa actatagaac aaagcaagat tatagaaagc aaaaatgaag aaggcaaaaa
    22801 agaagaatca aaaaatgaaa aaaataaatc tcacgaaaaa gtggaaaaag aacaaaaaga
    22861 tggaaaacaa aatgaacaaa aaaacgaaaa agaaagtaag ccagaaaata aaactgaaga
    22921 aaaggaaaag aaaaatgaga atgaaggaaa aaaagaaaat gaaaaagaaa acgcaaatga
    22981 agatggattg aaaaaagatt caataataac taggaaaata ataaaggaaa atcctgatac
    23041 tatttttgtg ttttgagata atgtagaagc caaaaaggct gatgcagaaa gtattgcaca
    23101 aaaatgagat aacgaagaaa tattagcaaa atcttgatga caagcaaaag aaatgagacc
    23161 agcgatcaaa gattgaaaaa aagatataga aaatagcatt tgaataccaa ccatgaatgg
    23221 tttttgaaaa catatgactg atcatgaatt caatgaaaac aaaaaagtta ttgatgatgc
    23281 tataagtgag attaaaaaag ctgctaaatg aaaaaagata catatacctt ttgatacaaa
    23341 aaagaatcaa tataatattt gaactggaat agctgattta aaaaacaaag ctccaaaaac
    23401 atttaagtat ctacaagaag aattaaaaaa actagagagt aaataaaaaa attgttggct
    23461 ttaacagggt tttatatggt gaaaaacaaa aatgaaagtt tctctactta ctccaaatct
    23521 tcttataata cccgatatca acgattcaga ttttgatttc atccaaaaag taattgcatc
    23581 gacatatctt tcttattatg aaatacaaaa aacacaattt atagttctaa aagataacga
    23641 agaaattatt gcttttggaa gaatctttcc tataggggaa aacgaggaag aattagggac
    23701 attatgggtt aaagaaagca tgagaggtca aaagttaggg attactttga tgaaagaatt
    23761 aataaaaaag aaaaaaaata agccccacct ttatcttgca tgtcaaaaag aacttcaaac
    23821 ctattatgag tatgctggat ttcaaaaaat caatactatt cctgaaaagt tgcactttac
    23881 tcaacaatgg gcaaaagaaa atggctactc tttcgtagta atgaaatact taagtgcata
    23941 aaagatgaaa taaattttta gacctcaaac ttaatcaata tacaaaactt tcccattaca
    24001 aaggaggggg tttttgaaag atcattatct ggggaaaata gaaacaacat ttaaatatta
    24061 aattaaaaaa atagactgga gcttgcaaaa aaacgaccat ccaattatcc tatccttttg
    24121 ataaggtaga taacaaaata ctttataaag agaattttaa aaaatctaaa cgaattatca
    24181 gtataaaaaa atgtttcaat actctgtact attagatatt tgggctatag attgagatgt
    24241 ccgattataa ttatttggtg gtatgtctca cttgaaaatt aaacttcaaa aaagtagtac
    24301 ttgcttttat tttacattta aatatatttt tatagaaaaa tttcttttat aaattgcaaa
    24361 aatggatata aaaaattttc ttcatcaaaa aaaagaaaat aaaaaaaaac aagttatgtt
    24421 actatcgatt gtattattta tggtcctttt aatttcggct ctgttttttt ttatggtcca
    24481 gtattatgaa aacatcacct ataatgtctg agatccaatt acagtagaat gaaaagtgat
    24541 ttctgataac aatttcccta tttatactca tagtatttcc aattctaaaa gatctttttg
    24601 attaataagt agtacagtaa atttaaataa ttttgtgaat aaaactctta taatcaactg
    24661 agaaataaat gagttaaaag acaataagtt tcccgtattg cagataaaaa acataaaaat
    24721 tcccgaatca aaagtaatca taagagataa tagatatttt tttacaaacg aattaatctc
    24781 tttcgatttt tcaaaagatt tagatcttag tgctgaaaaa actggtagaa acataaatat
    24841 ttattatcaa ggagagccta ttctttctat ggaaactttt gtttgtagca aaattacctc
    24901 aactcaaaat tgtgaagaaa tggtcatgaa ttacacaaaa aacttaaacg aaatgttcac
    24961 aacatatttg tgatatgttt tttacaaaaa taaagaaaaa actcgagtaa gttttaatga
    25021 tagcacaatg gggtacatat ttaagacatt tgataacaat gtgttactca atgtttcaca
    25081 tttaataaat ataatcaact ccaaatttat tatggaaaac aagcaagatc tgatattaaa
    25141 taactgtatt aatgaagaaa aaaacattag actagaaagt ataagtaatt tatctaagga
    25201 aattgtagat gaaaatttga taaaaataga agttgaatga ttggacaccg actgaaaact
    25261 tatgagttgc ggattaatag tagcaatatt ggaagatcga agtgtgaaga caaataaagt
    25321 atcatatttg gaataatttc ataaaataaa aaaaagaaaa ctttgagttt tcttttttgt
    25381 tatataaatt tctttttttt atgtaacttt gatgattttt actgattttc tatcatttcc
    25441 taatctcatt tttacttcat ctcatgcttt atgtcacttg atagcgtgtc aaatcggtga
    25501 gtctagagaa atacttaagg tgtcatctca atctatgccc acctctcatg ttcaaactat
    25561 tgtaacattg tatgtatcgt catctcattc aacttttagg gtaacttttg agccataatc
    25621 tactatgtca ctagctttag ttttttcttc ttttatgatt tcgacatttg ctaataattt
    25681 ttctaattct gctatttttg aattgataaa atctttgtcc tccatagccg acttgtattc
    25741 aaagttttca gaaagatctc aaagtgcctt cgcttctgac aatctttcgt gtacagcggg
    25801 aagttctacc tgtttataat gatttaattc tgtaactaat ttatcaaaac cttccttagt
    25861 tatatggttt actttttttg ttgccattat ttataagatt tttatataaa attactttgc
    25921 taatattctt tgtttaaagt tatcaaatgc gtgattaacc aaatcagaca aaatttcaaa
    25981 tcatttacga tcatattcca aaacttctcc atcaaattca aatctgaaat taccattata
    26041 gccattggta accttttcta taagtacctt gactacaact tctatatctc ctccttttgt
    26101 ataaattttt tttagactag aagacatttt tgaatcttct tttaataggt ttttttctac
    26161 caaatttttt aactcatttg tcataacttc tgtcaatcaa tctaatttat aaataacatt
    26221 tggttttcac atagttaaat atttatttaa taaaaccgta caagtaggat aaacaaaatc
    26281 ctaaaaaatt caacctaaaa ttacaattcc agttttttta aaaaatcttt ttttacatcg
    26341 ttgatttttt gtttgtctct gagaagaaca tatcattttc taaaaagctc tctaatatct
    26401 gtataatata ctaaatttaa aacctcttga attgtcgctc gtttgtgggt tcacaaatat
    26461 cattctactg gttggatatc caccaattct ggatctccat catatttaat taaaaaatat
    26521 accacctctt tattaaattt tcaattttta ttttctagtc actcaatcgt cgtagatcaa
    26581 atattttgaa taatactcaa aaagtttaat ggaatacctg tttcttcact aacttctctc
    26641 aaagcagctt tttcatcagt ttctcatatt tgtattttac cttttggggc aactcgttct
    26701 attttccccg aaagagcatg tcttttgata agcaaaaatc ttatatcatt attttcatca
    26761 taataatata tgattcctcc agcacttttc actaacttgt tcataactaa aatgaatact
    26821 aagctaaaat tttttatttt tctgttttca taatccaatt tactattcta agtagagacc
    26881 tagcaacggg atttttttct tcttcccaat tatatatctc atccaaaatc ttatctgctg
    26941 agctttggat agattctttt actgcttctg gacgaccagc aaacctatcc accatttgac
    27001 tatgtcttct taccactctc ttttctactt tagtgtctat tttttcaaat tccttttgcc
    27061 cagcatcaaa gaactctgat accttgtctt ttcatttttc actaattttt ttctgctcca
    27121 atcgagactc agctcaagat aacatacttt catgcaaaga ctctaaatta ccttttgtct
    27181 ttgatactat ttgttctttt tccacttgtt ctttttgttt tattttatct caagcaagct
    27241 ctctgatatt atcttcaaga ctagaaagtt catcaaaaaa atctgaatct tcattatgtt
    27301 catcaagatt tttttcatca tccaaacctt cattaaattc tatatcttta ttttctacca
    27361 taagctgttt gttaagcttg taaagttcgt agagttaaaa agttaataaa gttcgtaaag
    27421 tattaataac ttgataaact tgtaacttga tagacttgta actttcaact tgtaactttg
    27481 cgatgtgtat tttagcaccc tatcatacta acgactttgt ccaatattgc tacatttttt
    27541 ttgtaatcta ctctaattgg tcaaactata caaatcattc actcgtagtc ttccacaacg
    27601 attttggcat aaatacaaga aacaatagag tttttatctt gtataaatgt gtagtaaact
    27661 ttattttttt tcaaaatttt agcatctagt tgttttacaa gtcatcttgt ttcaataagt
    27721 tttacgatgt atctagtagt atcgtactct tcccttaaat catctctcaa aatattattc
    27781 attcataaat aataatattc atcatttttt aagaatccga caacaactcc atctgctact
    27841 gctcataaat tttctactaa gtatttcaat catgttctag cattatctaa atctagctct
    27901 tgttgttcca attctcaact catatagtta tctatataaa cagacaatca gtctaatgtg
    27961 ggaaccctcc cagaactttt ataaggttgg tacacaaatc atgctttttc taataaattc
    28021 aaatattttc ttaaagtaga gggagcgtat ttggtagaac ccaaagagtt gagtgacttg
    28081 gaaccaacag ggtctcaact agaaatatat tggtctatta ccaagtccaa aagtgccaaa
    28141 agtttatcat cttttttcat gagaaaaaat ttttcaaaat aaaccaagtt ttaattaaat
    28201 taataaaatt atatagattc aaaacaaaaa aacaatataa ataaacaaaa gttaattata
    28261 attcacaatg tatgatcaaa tacttgtaat taaaattttt atttttgaac taaatcgtgc
    28321 aatagaatga gaatttacac aaactagcca attttttttg aaacgaatcc aatcacgaac
    28381 tttctcaatg ttttcggtaa aaattcacag ttgaaaaaga caaataaact ttttattata
    28441 taaacaaata cgagaaggtg gtgtttggag cttgatcaac tagaattctt ctcaatattc
    28501 atctaagctt ttatttcact tatctatata atctttgaca acttctcata gggcctcaac
    28561 taagatgtcc accccttttt cctcatttac agatgaagaa ttttcatctt ttttgtaaga
    28621 aaaataagct ttgagatgct gtattttgtc tgcctgtttt tttggattgg gaatattttc
    28681 aaaaggaaaa cttattcaat aatttaattt aattgtaata tctcatctaa aaaacagctt
    28741 atctcgtaat catttttggg tatgtaaaat tgtttctatt ttatctcggt caaaaacttc
    28801 agttttatat tccaatagtc attctcgtaa atatacaaca attcctgttt cggtcataac
    28861 taaagcatcc aagtacatgt tcaaaaaatt tatacaaaat tttataaaaa ccataaatca
    28921 taaaccagca aaaattcgag ataaatattc ccacattata tatctatcca ataacaaaaa
    28981 aactaacatt aaaaccagta ataccaaaaa acactttaaa aatagtttca aaaaaacact
    29041 tccatgatga ccaattacat gatgaatttt agaagtgttg gtatgtcttt gaataacaaa
    29101 ttctcttatc ataaacgaat ctagtaccca atataaagta ccacatattt atataataaa
    29161 attaagatta ttttttcatg gcatctattt tcttttgttt ttccaaaaat ttctccattc
    29221 taccctttgc cattttctct actactctat ctttattaaa tactggatga cattgataac
    29281 atgtctcaat tttgataggt ccaggaactg tcgtgtttac tgtaaattct gctcaacata
    29341 tacattgaac agctacatcc ttgtaatatt gagaatgtat tccttttttc atttttaaat
    29401 ttcataaact aaaacttttt tgtattatct aatgaactca ctatatgata gttcatattt
    29461 aaatatttca tctttattaa accaatgttt aatttctaat tcagcttctt gtaagttggc
    29521 agaagcatgt attaaattaa ataccgacat tcatgactta ctgtcagcat atgtatatga
    29581 tatatgtgca aaatctcatc taatagtgcc tggagttgca gattttggct ctgtagatcc
    29641 aacgattttt ctaacatatt ctactgcctc cactcattca acaaccatag caagtactgg
    29701 tgtttttctc atgtattcta caactttatt taaaagatct tctcattttc tagtgcccaa
    29761 ttgtccaata ccctcataat gtcaccttaa aaattccaca ctaggggcca acatttttga
    29821 agcaacaatt tgtagccccg ctctctcaag acgactaata atttgtcaaa cgagggagcg
    29881 actaatggca tctggtttta aaacgactag tgtttgttga attgttcaca taacaaagaa
    29941 gttatatcat taaaacatag gtaaattgag gttagctttt tacttaaatt tttcaaggga
    30001 aaataaaaag aaaaatttag cttgaaataa acatataaat tagtataaag tattcatcca
    30061 ttttaatatg gttctttttg ttttacaaag aaacattctc taatgatggg gaatcgtcta
    30121 acggtaggac agcggacttt gactccgtca gtctaggttc gaatcctagt tccccaggaa
    30181 aaaataaaat gtataaataa atttaatata gcagtttaag ttaaaacaca ttgtataaag
    30241 gtatgaagtt tagagtttag taaacaatcg attataaact caaaacaatt aataaattta
    30301 ttatttatat tgcggggtag agaaacggta tctcgtcggg ctcataaccc gaagaaagat
    30361 ggttcaactc catcccccgc aaccaaaaat ttaatttgta ctgtaattcc gactttcagt
    30421 tttgtaaact gggatgtcgt ctaatggtag gacaacggac tctgactccg tttatctagg
    30481 ttcgagtcct agcgtcccaa aaatataaat tttaacacat aaatttaaat ctctaaaatc
    30541 gtggtgccga ggtggtggaa tggtagacac gcctggctta gaaccaggtg ccgtgaggtg
    30601 tgcaggttca agtcctgtcc tcggtaagta atataaagat tgaaaatcta ttgaaaatta
    30661 gtaaagaatc cttataggtt ctttcactta aacacattcc ggggtagctc agtcggtaga
    30721 gcaagtggct gttaaccact gggtcgtggg ttcgagtccc tcccccggag ggattattta
    30781 aaacttttaa atatttgtta taaacctgaa tgcctatcac aaaagcagca aaaaaagcat
    30841 cgaatagatc cataaaatta caccaaagaa attctgaatt caaacttaga ctaaaaatgg
    30901 ctataaaaaa atttaaaaaa tctctggaaa gatgagaaaa agtttctcct gaagatttaa
    30961 acaaaatcta taagtatgta gacaagtgtg taaaagtttg agtaattaaa aagaaaaacg
    31021 gttcaagaaa aaaatcgaat atggctaaat tgtttagtac aacacaaaac aaataatctt
    31081 tttgggttgt tttgccggtg tggctcaatg gtagagcaac cgccttgtaa gcggtaggtt
    31141 accagttcaa gtctggtcac cggctccatt tgattgaaga aggaaattca aaaaaataaa
    31201 acctaactgc attttgtatg tattttacta tggggtggta gttcagctgg ttagaacgcc
    31261 tgcctgtcac gcaggaggtc gagggttcga gtcccttcca tcccgccatt aaaatccatt
    31321 tatttggtct catcgtctaa cggttaggac acgggacttt caatcctgca atcggggttc
    31381 gattccccgt gggaccgcca tttatattaa ttgattagcc cgattggcta gtcccgcaat
    31441 actgcgggat tacaagcagg ggacataggg gtaatttaat tgaaatgggt gattagct
//
LOCUS       DAOSID010000004        28523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_1847_length_28523_cov_14.823064, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000004 DAOSID010000000
VERSION     DAOSID010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 28523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28523
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1847_length_28523_cov_14.823064"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PK674_00585"
     CDS             <1..356
                     /locus_tag="PK674_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15061.1"
                     /translation="GTGIGGGIGGGTGIGGGINFDDEDGDGIPDHLDACPTIPENYNG
                     IEDSDGCPEIDVDKLNDEEANITAKECFSCPCNFSDFANDLNIKDKVKAILWDFGMTT
                     IYSETIPENIRQFLQ"
     gene            complement(392..1243)
                     /locus_tag="PK674_00590"
     CDS             complement(392..1243)
                     /locus_tag="PK674_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="HemK family protein methyltransferase"
                     /protein_id="HOG15062.1"
                     /translation="MKIQELLQNQEYTQKRVLENLICYFLDISRENLRISLDQDIEES
                     VINKIIQAYDDFVVQKKPLEYILGYVEFMRNKFKIDHRALIPRPETEYMIQAVNEHVS
                     TLKPGNNILFDIGTGCGVLGLSVLLHNPSFFTEAYLSEYVDYTLSLAQENFEIFKPRL
                     GKTKVNLVQSNLLNFVKDISLSDSNLILVANLPYIPENTFDQNVGENVKNREPKPAFV
                     GGEDGLDYYREMFQQIFDFGIKPTMFLEMMTRQVDVLRKEFGEKIKFQETKTFHFNIR
                     IVKARIK"
     gene            1429..1734
                     /locus_tag="PK674_00595"
     CDS             1429..1734
                     /locus_tag="PK674_00595"
                     /inference="COORDINATES: protein motif:HMM:NF021402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF2103 domain-containing protein"
                     /protein_id="HOG15063.1"
                     /translation="MRVKFQKVKTEHNILSEFTKFLLEVEKDPNIDRIIPGRISRQQK
                     GSSQLMFRITIPTNSGFKCIMSKGSTAQELFVICTPENIEKVKKNILLNVEKYLIKS"
     gene            1766..2443
                     /gene="trmD"
                     /locus_tag="PK674_00600"
     CDS             1766..2443
                     /gene="trmD"
                     /locus_tag="PK674_00600"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:NF013874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOG15064.1"
                     /translation="MKIYYVSIFPEIFDSFLETSLIKKAQEKDILDFEIINPRDFCTD
                     KHKQIDDEIYGGGTGMLMMAQPVIDSINSIIKKLKSKENFQIIFPNPSNIFRNQQSAH
                     SFSKTENLIFVCARYEGIDHRFVQYFQDKYPDNFHQISIGKFVTLGGEMPAMTMTESV
                     VRLIPGVIKEESSRQEESYSISQNMENIEHPQYTRPQEVEGYSVPEVLLNGNHKEIEK
                     WKQENSH"
     gene            2646..3242
                     /gene="rdgB"
                     /locus_tag="PK674_00605"
     CDS             2646..3242
                     /gene="rdgB"
                     /locus_tag="PK674_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOG15065.1"
                     /translation="MRKIILASNNQHKIKEFKEIFFDAEILSLNDIGYCDDIVEDGET
                     FLENALIKSNIVHEFLKEKGIEADVIADDSGLCVNALNGEPGVYSARYSGGHGNNADN
                     RAKLLKKLEGKNDRSAYFVCCLVKMFPSGEYIYSEGKTYGTILKEEIGDKSFGYDCIF
                     LSDDLHKSFGEASDEEKNKVSHRGRAIANLIEKMKTRE"
     gene            3318..3677
                     /locus_tag="PK674_00610"
     CDS             3318..3677
                     /locus_tag="PK674_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="four helix bundle protein"
                     /protein_id="HOG15066.1"
                     /translation="MGNVEDLKVWDGSIKLAKNIYFLIKDNPNIQRDFGLKEQLQKSA
                     ISVASNIAEGCNRGTDKEFIRFLYIARGSCSELKTQLIISKEIAYIEKTICDKLCADI
                     DNINKMINGLIQKLSKH"
     gene            3773..4942
                     /locus_tag="PK674_00615"
     CDS             3773..4942
                     /locus_tag="PK674_00615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="glycosyltransferase"
                     /protein_id="HOG15067.1"
                     /translation="MQDKKLGILFFGRLDDEKGFGLILDMLKAFIKKHGTIPFSFYVF
                     GKGKYVNDLIELASDHSSVHFFGWQTLSTIQRYQENCDFCLMPSTFLETFGLTAVNSL
                     SMGIPVIGFAKGGLTQFIQSKYDISKAEGQTDAEKLYNKMSEIIDQYNKGGINIEAER
                     KKAKTLANKYTVEKWLENITPIIGKPKRILMVSDFRNKLGGIETYIHDVKDILESKGY
                     EIKIYGAKIPSGKLGQLCKYFGLFFAIFNFVDAIRIKIVSKKFKPKIIWYHSTLRFLG
                     RLPIKKLSTHDSKKIMMYHDLGYFHPYPSLVTQENMVKTPLNLKNFVKSANTKNPFKI
                     LAICFKYCSVRLLSKNLKESIDFHLVPSNFLEKIVSDSYQINPKKIQTLSHFIQK"
     gene            5269..8457
                     /gene="secA"
                     /locus_tag="PK674_00620"
     CDS             5269..8457
                     /gene="secA"
                     /locus_tag="PK674_00620"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOG15068.1"
                     /translation="MLDRIKKLFGMNYTEKEINKLKLVVREINQYFQEYEKLSDEQIK
                     AKTEEFKKRVQEKGESLDSILPEAFAVVKQAAKRMCGQTVNVKGEKVLRNMIPYDVQL
                     VGGIVLHQGKIAEMKTGEGKTLVATLPVYLNALEGKGVYVVTVNDYLASRDTEWMGHL
                     YQRLGLSVGCVVKGVAIQDRRGEYEKDITYVENSELGFDYLRDNLVKSINQRVLTRRP
                     LNYAIIDEIDSILIDEARTPLIISEAREEATEKYQYYAKIVQTLTPCSGKKRVSKGLL
                     YELMNEDAKKESVEDGDYYIDEKTKTVSLSGEGIQKLEKMLGVENLYKDIGYEEIHHI
                     ENALRAKAVYERDKEYIVKGGEVLIVDEHTGRTMPGRRFSEGLHQAIEAKEMVEIKRE
                     SQTMATITYQNFFKQFGKLSGMTGTAVTEGEEFSKIYNLDVLEIPTNKETIRVDRHDK
                     VYFNQNAKRKFVKEDIKFYHEMGQPMLIGTANIATSEYLSKILEKEGINHYVLNAKFF
                     EQEAHIVSNGGKFKSVVVATNMAGRGTDIKLEKGLNEKLANNYVKRAEKNVKNWDIEY
                     NIYSSKELELTLEAIKQIFGIDDEAIRQMGNGGIEIKQTKISTKFNTSRKRKTEVFAK
                     IYFTLVGGPSLPNITKDLQYGLFVLGTEKHESRRIDNQLRGRSGRQGDPGKSVFYVAL
                     DDLIMRKMGGEKIMGIASMLMKKEELENLELTQKQFTSAIERAQKQIEAWHFSIRKHL
                     FDYDSVIDKQRQRIYKKRDEILASELDEELKKEFVKNTKKDLRDNIDLIINVKINEAK
                     NLKQTNIEFLETLIKEFNIKLDKKTADKWEAMGFNDLELETIETLGKYLEEKLKKLDD
                     DKLYDIFRDVLLHHLDKLWVDHIDEMQYLRDKVGFMGYAQQDPLIVYKKESFEKFQSL
                     IYTFKSTTTSYILNLDFDAIQKQDEIAKLILEKQKSGDKEFLSKLSKVSGNLQELIKL
                     AKQEEVKRAENIKREMIFEDEDGFEVFEVGDEKGETKNDSGKPIENTKIRPNDKVTVK
                     YKDGKMEYSIKYKKVKDDVLAGKCEVIKINEN"
     gene            8487..8852
                     /locus_tag="PK674_00625"
     CDS             8487..8852
                     /locus_tag="PK674_00625"
                     /inference="COORDINATES: protein motif:HMM:NF019401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HOG15069.1"
                     /translation="MGNFSNLPFGHYIGFLLEIIITIIYVSGIMVIIIGFIKSLFKFI
                     RIIFTKNTDSYNHKNEIRMFLGNYTLLGLDFIIIADIVHSVINTEIQQLYSLGIIVII
                     RTAIAYFLGKELKEIKNKK"
     gene            complement(9015..10952)
                     /locus_tag="PK674_00630"
     CDS             complement(9015..10952)
                     /locus_tag="PK674_00630"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="ribonuclease J"
                     /protein_id="HOG15070.1"
                     /translation="MMSENNLFGNLNIDFSKKNKQKRGRQNQQVNGALDKDLTSEAFL
                     RHLGKTELDDIKDPHKLIKKGEKPVKIGSLMGLEQVGQCMFVEYENDLIIIDAAMEFT
                     THANFGADYIIPDISYVKKNIKKLRGIIITHGHLDHVGSLRDILPELGYPMIYTTPLS
                     LGIIKKTFENPKDSEKIRYKIIDPDVDILKLGCFTLEFVPVNHNIPETLAVSIHTPKG
                     IIFNSADFKIDHTPAIDRPADLAKIARIGAEGVKLYIGDSLGSNKKGRSISEKEIGEN
                     LDELIQKTKTRILIATFASNVGRIIQIVNSAIKANKTIFLSGRSMVNNIEICQQLGYI
                     KVPKGYIRKLNEEVDTFPDDQVLILSTGAQGEEFAALTRMSRNEHNFLQLRKGDTILM
                     SATPIPGNEGSVNTMINNLIFKDIHLITNDDIDVHASGHGCMEDHKLMLSLLRPEFFL
                     PYFMPAKERYAHKNLAMDMGMPESRILMPMKNGEIIEMYDDVVLIGKEKLKLDTVMVD
                     GKGKGHMSGEYVIKARSIMAEDGVLALIFKVDAKSKELVGNIQIESRGFVYSSEVKDI
                     HTKVVEFARAKYVENSKRKMPVKDNLKILKEDLGEFIMQIIGRVPMIMPMFVYINKDS
                     KDDVSRDEAILGMTLDEQGGE"
     gene            11009..11551
                     /locus_tag="PK674_00635"
     CDS             11009..11551
                     /locus_tag="PK674_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15071.1"
                     /translation="MNLSLLRESCIDRNIPIISIETEDFLSKLLEQHKPKVCLEIGGA
                     VAYSSIFIANKIKERDGILYSFEISYNDYLEGIQNVGKYELKNLTVYPFDFLKIDIKK
                     LIPNTLDFVFVDGQKSQYANYLMNIFDMLSTKSILVCDDVIKYKTKMFGLYEFLEKKQ
                     IIYDEINTEEGDGVLLIKKE"
     gene            complement(11661..12122)
                     /locus_tag="PK674_00640"
     CDS             complement(11661..12122)
                     /locus_tag="PK674_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15072.1"
                     /translation="MVNIKLESEGYLIENKILFGASIPNKEYINLATEIGEDIVIEKL
                     PYSIVEYPGEYDIDGVFIRSFGSKDGKLNYIITINNKTIGLVQSAKILEEDDVRSLDA
                     WLYLDDAVEKKLEQLELEGKKFKLNLEDGNLRIEEKESEHAEVMIDESEDE"
     gene            complement(12155..13489)
                     /locus_tag="PK674_00645"
     CDS             complement(12155..13489)
                     /locus_tag="PK674_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15073.1"
                     /translation="MKKIAIRICLSIFVLGFFSLGLTIVYGEDYSSSDMCSSIPNYSN
                     NSNSGTSYATNNNIYAWYINSPSAISLATTHPGSPNVCSDIELATSRVERKQQLCTNN
                     LDELKTYQETLLGKYKIHRSYQFERGFDSAKKVWGLQNLMKEADCQDHRDCKLGVVTI
                     GDFLFCNICSAIDPDRKEPTSISPQVSCPVSYEPILDNLCCEKVEAGLECQIKKNWGQ
                     CTNSDTFGLEVGATDLSKLNEYTCEQSLEEESLPFCAGCDKIPESSKSCEESYGEGWY
                     DDNDGCCVNCGQNKQRSANENSCTCTLSEDDCINKGKKLNSNTCKCECDPSRECCGIL
                     LNTVVPFIGDCIEMTSQDDPGSSNAPNTTTINQLNAFPFLMMGLSKILVTIILIFSFL
                     IIIVAGLMMTTGVYSESNYKKGMERIQKVVVGLILLGASGLILKLINPSFFG"
     gene            complement(13525..13977)
                     /locus_tag="PK674_00650"
     CDS             complement(13525..13977)
                     /locus_tag="PK674_00650"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOG15074.1"
                     /translation="MSLQEKLFADYMQAMKDKDVSKKAILNYVIAQIKNKKIELQKDL
                     EDGEVIKILKKEIKALVEAIGFLGQANKLKELKEEQAKKQVLEQYLPQTKSKEETKEI
                     VLNLIKELGITELTKQRGQVMGAIKSQYGEVVEGSLANEVINEILKGS"
     gene            complement(14042..14123)
                     /locus_tag="PK674_00655"
     tRNA            complement(14042..14123)
                     /locus_tag="PK674_00655"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14087..14089),aa:Leu,seq:gag)
     gene            complement(14704..16104)
                     /locus_tag="PK674_00660"
     CDS             complement(14704..16104)
                     /locus_tag="PK674_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOG15075.1"
                     /translation="MDLQKFTQQLQGGEVSIPLIKEGNKIRGIILKKIENGILVECDN
                     GAFTGVILSKEVKELVRSGYDLAPGREIELEVVNTNIRHEDGYYIVSVTKLLQYDVRK
                     SILAKFEKDEIIKVVPTEANLGGLLIDMHGIKGFIPLSQLAPIHYPRVEDGDQEAIFD
                     KLLELIGKEISVRIINIDEEEKRIILSEREALKEERDKVLKELAVGKTFDGIVSGVSS
                     YGLFVTIGGTVEGLVHISEITYGHVTNIDRLGKIGDKLQVKVIGLENGKISLSSKKLK
                     EDPRSALPKKYKVGDIIEGEVVRFVPYGVFIRVFEDINGLVHLSELSQKSVQNPNEVV
                     KLGEVVKAKLILLDPKNRKIGLSMKDSTPGIDEDGKIAKKIIKKPSKGLEDVVKMLEE
                     EKAEEKIKKIKEEENSKQELKKEEKKEKKEKKEKEEKEGKEEKKKKEEKKEKEEKKEK
                     EEKKVKKEKKEQEEKK"
     gene            complement(16208..16462)
                     /gene="rpsO"
                     /locus_tag="PK674_00665"
     CDS             complement(16208..16462)
                     /gene="rpsO"
                     /locus_tag="PK674_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="30S ribosomal protein S15"
                     /protein_id="HOG15076.1"
                     /translation="MAKKKSENFFQRHENDTGSPEFQIGTLTEEINMLQGHIEINPKD
                     FDAKRSLLKKVAKRRRLLKYIKNQDLDSYSNVSKKIGVKV"
     gene            complement(16568..16640)
                     /locus_tag="PK674_00670"
     tRNA            complement(16568..16640)
                     /locus_tag="PK674_00670"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16604..16606),aa:Arg,seq:cct)
     gene            16835..18568
                     /locus_tag="PK674_00675"
     CDS             16835..18568
                     /locus_tag="PK674_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15077.1"
                     /translation="MNQNKSKDSMQFIYPDRYQEKFLDKKKKYPYSSGCIQRIISITL
                     DTLNLQNTYKNLPVIVTKLLEEPKNLTEEEKEMMRKVSENGDLVRAIQNIYNKKVIFS
                     KKENIIDFGDYLKKYLEITGIDFDILELCGQYNYMSKSKDDPIILMDGEAIRLQKIQT
                     GLSHGKGKAIWRDKNREIVKAKKRNQEFEISWVLIDSRVNLIDNGEIEKIYVLDKNGN
                     EVDVYWDTNREIVEVSKGEERFEICGNIDHNTVRLVNGEKVQKVTIRNNEGEKRDVFI
                     NKNKEIVEIKIGDEKFEATGFCTEDLIGNEILQHITVRGANISNVFLNKYMEPIKITK
                     GKEELTLSYVYYGTETLISGEEVQKIKASDMLGDERDVVINKKKKIVEVSKGKENFEI
                     CDFYREKEENIINGEAVQKVKIRSKKGNERDVVINKYKEIVEIKIGDKKFELCGIGQK
                     SMDLIGGISLQNVFIRNKLGKVENYCLNRNGEVIMLNKGKEEVVLHATQGIKHLGNQN
                     LVQEVTIRDNQRKTHKAVVNENNSTYTYIDLNGKEKYFDGDCDVLFTTDLSKPLFQKG
                     ITAEGVIIIDN"
     gene            complement(18612..20552)
                     /locus_tag="PK674_00680"
     CDS             complement(18612..20552)
                     /locus_tag="PK674_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG15078.1"
                     /translation="MSEYISNSVDFGCNINISKIENKKCPTNKIKIMRRIIERLKKED
                     MKNTNPKKDKIETKYNYKKALQYFFLTYKQFLRNGLFSFILRILCSGLSIVPPLFYKK
                     IFDIISQSGITSTELSAHAISILVVLFGIQMGSFVAYRIYDFFTITFQMDVQERLNNK
                     FLKKIQKQSFQFFTDNFAGSLVSKYRKGVSAFERLSDIVNWDIIPFIVNVLLILIIIG
                     FQSYWLSLVLLLLIILFSCVQYKLYVYIQPYQERANTLDSNLGGLYSDIITNNTTIKF
                     FASESREEKEFAKLNHEVFKSKKAQYFRSMIIRGTSGLFSIFLQVGIMYIALRMRGNG
                     IISIGMIVLLQAYVLRIVDFMRGIGQTLRRVFYSITEISEIVALVETIPDIQDKSDKE
                     LHLSSGEIKFEKVGFSYGDNQIFKNLSLHIKSGERVAFVGESGSGKTTITKLLFRLYD
                     IQSGKINIDGQDISQVTQNSLRSSMSMIPQDPILFHRSIKENIAYGRPDASEEEIIAA
                     SKMAKCHNFVSNLKYGYDTLVGERGVKLSGGERQRIAIARAILGNKDIIVMDEATSSL
                     DSESEALIQEAMEEVMRNKTTIVIAHRLSTVMKMDKIIVMDKGKVVEKGSHKQLLSQN
                     GIYKKLWNIQSGGFLGNILHKI"
     gene            20673..22097
                     /locus_tag="PK674_00685"
     CDS             20673..22097
                     /locus_tag="PK674_00685"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="glycine--tRNA ligase"
                     /protein_id="HOG15079.1"
                     /translation="MGFSLETIVSWAKRKGFVYPNSEIYGGLANAWDFGPYGSLLKKN
                     ISDLWIKHFVQERDDIVFMDTAIIAHPTTWQASGHLASFADALIDDKNTGQRFRADKI
                     IEDFFEKNREGLNDEDLAKKLGVTNLSPESWGNEGMRDFIVKNIPNNPETGKPANWTE
                     CRNFNLMLDTHLGPVANDSSKVYMRPETCQSLFTNFRSILDSTRMRIPFGLAQTGKAF
                     RNEITPGQFLYRTREFEQMEIEYFVENDENKAMETYEQRKKDSMEYRTKVIEVNPENL
                     RFKDHEKLAHYAAAAVDIEYRYPRGFGEIQGVHSRTDFDLKQHQDTSGKDMQYSDPFT
                     GKRYIPRCVEASWGLGRSVMVTMLEFYDEESLENGESRVVVRFPKQLAPVKFAVLPLV
                     KKDEKQVEIAEKIFKDLSKKFKCEYDDGGAIGKRYRRQDEIGTPFCITVDPQSVEDGT
                     VTIRDRDSMQQKRVKIEELEGFMG"
     gene            22231..22461
                     /locus_tag="PK674_00690"
     CDS             22231..22461
                     /locus_tag="PK674_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF6290 family protein"
                     /protein_id="HOG15080.1"
                     /translation="MTTQTISVRVDSNVLEAVKKIAKLNGLSLSSVINVKLREFANNK
                     ELRLIDDEEFEIDFGEKGAPIDDVIEYLKSID"
     gene            22463..22705
                     /locus_tag="PK674_00695"
     CDS             22463..22705
                     /locus_tag="PK674_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15081.1"
                     /translation="MVEQRKKDLEKISKAGRNEILKIACLIRDGRTVGLDIKKIQGKQ
                     NLYRCRYGKYRIIFSILCNKIQVIRVGNRGNIYKGL"
     gene            22750..23088
                     /locus_tag="PK674_00700"
     CDS             22750..23088
                     /locus_tag="PK674_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15082.1"
                     /translation="MKEIKLSEENYKALLKSLAIGNFIYGIMADIVNDFYKKDAHDSD
                     EVIHQVLKCCDDPKVKDIWDGKNVFTEEYEDNIWNDIQEYENYVVEDLTGIDVEELNQ
                     KIDEEMGKHI"
     gene            23153..23482
                     /locus_tag="PK674_00705"
     CDS             23153..23482
                     /locus_tag="PK674_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HOG15083.1"
                     /translation="MEVKELSDFIDRTLTKGRYPALNYSIRERVFAQTLKISEEVGEL
                     SEQISGKFGGQRLDKIDKFSDERLGDELADVIFSTIRLARLLDLDVDEILSKKMKILR
                     ERFEKEN"
     gene            23511..23732
                     /locus_tag="PK674_00710"
     CDS             23511..23732
                     /locus_tag="PK674_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15084.1"
                     /translation="MNKKLLFIGLGTILIVGTINTIIDIQAMLNCNGCSAPWQTALIS
                     TGFLYIIIGAIYWFIYLLLSHFKKIKKYG"
     gene            23849..25306
                     /locus_tag="PK674_00715"
     CDS             23849..25306
                     /locus_tag="PK674_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15085.1"
                     /translation="MNNNSINLNYGGERGKINTSTSEKSQQIYNIKQNLNVISKNISY
                     LTSLNKTNKDIFDRIKKQIFNLEVANDRLTVEINSIKNEICTILLNTNLDLKIDLQKK
                     KDLIKQKENEILINGDKHKILSQRLDAISKEIDGIENEINQEIKDKIFYLYMSQLVNF
                     REVSTDITIDDLTIKPEGLREFVNSYLNEDFPKDLLDKVIFLKGDKNTGKHSVVKAIA
                     NELNRPIFRIKYDDYFDEEGLYVIFGLITSYLRQQREEKNEQIKQYNKLLRQIKKIKY
                     GTLAKVNIYKIEDGEGNSYTFDIGTKKGKSGCLLKLKGFVDNIKHTIDSIEDSCVLYV
                     DDLDRIIQSSKYEKGELLGPVKMIINDIKSENYDVILVLAGNGLGINHGDFKLGIDRV
                     FQFNGIEENYQRLFEEMLQKKLKKLNIKHNIGNISLNGLDRQYRNVKFLDKLINRVLK
                     NNILNGKVITQEILELELNDLIEGEKSLYDGIGLK"
     gene            25384..25839
                     /gene="smpB"
                     /locus_tag="PK674_00720"
     CDS             25384..25839
                     /gene="smpB"
                     /locus_tag="PK674_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOG15086.1"
                     /translation="MKIISKNRIAYHDYSFEKKYEVGIVLLGHEVKSVKIGQINIKDA
                     IVRIQNRELWIKNMDIPLYKKTSPILAPGYQAKRDRKLLINKRELAKISALTDQAGNV
                     IIPLKIYINDKGLIKLEIGIGKLMRKIEKKQILKEKDIKRDMDREMKRR"
     gene            complement(25996..27090)
                     /locus_tag="PK674_00725"
     CDS             complement(25996..27090)
                     /locus_tag="PK674_00725"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF2304 family protein"
                     /protein_id="HOG15087.1"
                     /translation="MSLLQFFIIIVAIIFLFFGIDLYKRKRMNILHFFVFLLGSLAII
                     VFAINNELLNKFGTFFGLARGADLIVYASLVALAYFYVTLFNKYTKDKHELTRLITHL
                     SIDKTYELEKENIKNFKNKTEKDDFVFNIRVYNEGKVVGSVIDEIIEAGFKKMVFIND
                     GSKDNSLEILNEKKKQYPDCLFIILSHTINRGGGAANQTGYKFIQKYSDELKIKRFVG
                     FDSDGQMDIKDMETFISEIQKDRGKYEAYLGSRFIQGGKAENIPFFRKMILVISRLVT
                     MIFYNAKVSDPHNGYRVIHIDSFKKIKIMADGMHYANELNEQIKKHKIKYKEIPVHIR
                     YTEHSLRKGQKNSNSIKLAVEMIHKKIFFR"
     gene            complement(27274..28338)
                     /gene="galE"
                     /locus_tag="PK674_00730"
     CDS             complement(27274..28338)
                     /gene="galE"
                     /locus_tag="PK674_00730"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOG15088.1"
                     /translation="MEEQSRSILITGGLGYIGSHTSTVFLEEAYDIIIVDNLSNSDIS
                     VLDKIQTITGKKPKFYNIDIRNLEELEKVFKNHPNIDGVIHFAAKKAVGESCQDPFLY
                     YDNNVIGTNNLLKLMQKYDKKNIVFSSSATVYDSPKLLPPFSENDRVGTYNPYGTTKL
                     IMEYMLKDLATFKGFNVVNLRYFNPIGAHHSGLIGEDPKGIPSNLIPYILKVANGEIE
                     YVQIFGDDYDTPDGTGVRDYIHVMDVAEAHLSAFNQILKYHEYLLENKIDPDKGYFDV
                     FNIGTGDGKSVKEMIELVEKVTEKKIPYKIVSRRPGDIDTSIANVQKAKQVLGREYSR
                     SMYQAIEDAWRFTQKGNSNS"
BASE COUNT        10554 a         3817 c         4218 g         9934 t
ORIGIN      
        1 ggggaacttg aatctgagga ggaatttgag ggggaacttg aatctgagga ggaataaatt
       61 ttgatgatga agactgagac tgaataccag atcatttgga tgcctgccct actataccgg
      121 aaaattacaa tggaatagaa gactctgatt gatgtcctga aattgatgtt gataaactta
      181 atgatgaaga agctaacata acggcaaagg agtgcttttc ttgtccttgt aatttttcgg
      241 attttgctaa tgacctaaat ataaaagata aggtaaaagc tattctttgg gattttggta
      301 tgactacaat atatagtgaa actatcccag aaaatataag acaatttttg caataaaatt
      361 tatataaaaa atccccatat tacacaaaaa cctattttat ccgagccttg actattctaa
      421 tattgaaatg aaaggttttt gtttcttgga attttatttt ttctcaaaat tctttgcgta
      481 aaacatctac ctgccgtgtc atcatttcta aaaacattgt gggcttgatt caaaaatcaa
      541 aaatttgttg aaacatttca cgataataat ctagtcaatc ttctcatcac acaaacgctg
      601 gctttggctc tcgatttttt acattttctc aaacattttg atcaaaagta ttttcaggga
      661 tataaggaag atttgcaact agaattaaat ttgagtctga taacgaaatg tctttcacaa
      721 aatttaatag atttgattgt actaaattta cttttgtttt accaagtcta ggtttaaata
      781 tttcaaaatt ttcttgagct agagaaagcg tataatctac atattcactt aaatatgctt
      841 cagtaaaaaa acttggattg tgcaataaaa cagacaatca taaaacacca caacctgtcc
      901 caatatcgaa taatatatta tttcaaggtt ttaatgtgga aacatgttca tttactgctt
      961 gtatcatgta ctctgtttct gggcgaggaa ttaaggctct atggtctatt ttgaatttat
     1021 ttctcataaa ttctacatat cataaaatat actctaaagg ttttttttga acaacaaaat
     1081 catcataagc ttgtattatt ttgtttataa cactttcttc tatatcttga tctaaagaaa
     1141 tccgtaaatt ttctctacta atatccaaaa aatagcatat caaattttcc aaaactcttt
     1201 tctgtgtata ttcttgattt tgtaatagtt cttgaatttt catataattt agttgaatgt
     1261 tatagattta aaattataag tttattagtt tgtaaagttg ataaagtttg taaagttgat
     1321 aaagtttgta aagtttgtaa agtattaatt aacttgtaaa cattataaac ttgtaacatt
     1381 ataaacttgt aacttttaac ttgtacttta tttaatcata ttttcaatat gagagttaaa
     1441 tttcaaaaag taaaaacaga acataatatc ttatctgagt ttactaaatt tttgcttgaa
     1501 gttgaaaaag acccaaatat agatagaata attccaggta gaatttctag acagcaaaaa
     1561 ggaagcagtc agcttatgtt taggataaca atacctacaa atagtggctt caaatgtatt
     1621 atgagtaaag gatctactgc acaagaattg tttgtaatct gtacgccaga aaatatagaa
     1681 aaagttaaaa aaaacatctt attgaatgtt gaaaagtact tgataaaaag ttagttgtag
     1741 ggatttatat cacacaaagt ttattatgaa aatttattat gtatctatat tcccagagat
     1801 ttttgatagt tttttggaaa cttcattgat caaaaaagct caagaaaaag atattttgga
     1861 tttcgaaata atcaatccaa gagatttttg tacagataaa cacaagcaaa tagatgatga
     1921 aatttatggg tgagggactg ggatgttaat gatggcacaa cctgttatag attcaataaa
     1981 ttcaataatt aagaaattaa agagtaagga aaatttccaa attatttttc caaatcctag
     2041 caatatattt cgaaatcaac aatcagctca ttctttttca aaaacggaaa acttaatctt
     2101 tgtatgtgct aggtatgaag ggatagatca tcgttttgta caatattttc aagacaaata
     2161 tcctgacaat tttcatcaaa tatctatttg aaagtttgtg actcttgggt gagagatgcc
     2221 agcaatgaca atgacagagt ctgttgttcg cctaatccct ggagtaatca aagaagaaag
     2281 tagtcgacaa gaagaaagct attccatctc ccaaaatatg gaaaatatag aacatccaca
     2341 atacaccaga cctcaagaag tagaatgata ttctgtacct gaagtattgt taaattgaaa
     2401 tcataaggaa attgaaaaat ggaaacaaga aaatagtcac tagtcaatag tcgatagtcg
     2461 ctactcaata gtcgttagtc ttatagtcgt tagtcgttag tcgatagtca aaagtctata
     2521 gtcaatgatc gaaaatattt tacttgacac acagttgaaa tactaaatat tgaagattta
     2581 aaagtttgta atagcattta gatttaaaat tagaattatt taccgttttt tatcttataa
     2641 gcatcatgcg taaaattata cttgcttcca ataatcaaca taaaataaaa gaatttaaag
     2701 aaatattttt tgatgctgaa attttatctt tgaatgatat ttgatattgt gatgatatcg
     2761 ttgaagatgg agaaactttt ttagaaaatg cactaataaa gagcaatatt gttcatgaat
     2821 tcttgaaaga aaaatgaatt gaagcggatg ttattgctga tgatagtggc ttatgtgtaa
     2881 atgcattaaa ctgagaacct tgagtttata gtgctagata ttcttgatga cacggcaata
     2941 atgcagacaa tagagctaaa ttattaaaaa aattggaatg aaaaaatgat agatctgctt
     3001 attttgtttg ctgtctagta aaaatgtttc caagttgaga atatatctat agtgaatgaa
     3061 aaacatattg aactatattg aaggaagaaa tatgagataa atcttttggt tatgattgta
     3121 tttttttatc cgacgattta cataaatctt tctgagaagc aagtgatgaa gaaaaaaaca
     3181 aagtttcaca tagatgaaga gctattgcta atttaattga aaaaatgaaa acaagagaat
     3241 agtcgttagt cgctagtctt atagtcaata gtctatagtc aatgatcaaa aatactttat
     3301 ttggtacaat aactaaaatg tgaaatgttg aagatttaaa agtttgggat ggttctataa
     3361 aattagctaa aaatatatat tttttaataa aagataatcc taatatacaa agagactttt
     3421 gacttaaaga gcaattacaa aaaagtgcga tcagtgttgc gagcaatatt gcagaatgat
     3481 gtaataggtg aacagacaaa gagtttataa gatttttata cattgctaga tgaagctgtt
     3541 ctgagctaaa aacgcaactg atcatatcta aagaaatagc ttatattgaa aaaacaattt
     3601 gtgataagtt atgtgctgat atagataata taaacaaaat gattaacgga ctaattcaaa
     3661 aattaagtaa gcactaattg aactattaac aactaatgct ctattgatga gcgactagtg
     3721 actattgaca aacgactagt gactaatgac tttaaatact taaaaaacac ctatgcaaga
     3781 caaaaaatta tgaatattat tttttggtag actagatgat gaaaaatgat tttgactaat
     3841 tttggatatg ctaaaagctt ttataaaaaa acacggaact attccttttt ccttttatgt
     3901 ttttgggaaa tgaaagtatg tgaatgacct aatagaatta gcctctgatc atagttctgt
     3961 acactttttt tgatggcaaa ctttgtctac aattcaaaga tatcaagaaa actgcgattt
     4021 ttgtctgatg ccatctactt ttttggaaac ttttgggctg actgcggtaa attcactatc
     4081 tatgtgaata cctgttattg gatttgccaa atgatgattg actcaattta tccaaagtaa
     4141 atacgatatt tccaaagcag aatgacaaac agatgcagaa aaactttata ataaaatgtc
     4201 agagattata gatcagtaca ataaaggcgg aatcaatata gaagctgaaa gaaaaaaagc
     4261 taaaacatta gcgaacaaat atactgtaga aaaatggctt gaaaacataa cgccaatcat
     4321 ctgaaagcca aaaagaatac taatggtttc agattttaga aataaactat gatgaataga
     4381 aacatatata catgatgtaa aagatatttt ggaatcaaaa ggatatgaaa tcaaaatcta
     4441 ctgagccaag atcccaagct gaaagttggg acaattatgt aaatattttg gattattttt
     4501 tgcaatattc aactttgtag atgctattcg cataaaaatt gtatccaaaa aattcaaacc
     4561 aaaaataatt tggtatcata gcactttgag atttttagga cgattaccga taaaaaagtt
     4621 aagtactcat gattccaaaa aaattatgat gtaccatgat ttgtgatatt tccacccata
     4681 tccaagtcta gtaacacaag aaaacatggt aaaaactcct ttgaatttga aaaatttcgt
     4741 gaaatctgct aacacaaaaa atccattcaa aatcttagca atttgtttta aatattgttc
     4801 cgttagatta cttagcaaga atttgaaaga gtcgatagat tttcatttag tgccatccaa
     4861 ttttttagaa aaaatagtat ctgattctta tcaaataaat ccaaaaaaaa tacaaacact
     4921 ttctcatttt attcaaaaat aaacaaaata ctgtaattaa tatcatgatg aataaatgag
     4981 atagtcatag gtaaatttac actaatttaa tctttaataa attaattatt aaaatctaca
     5041 aacaaaaata ttttatttgt gaacttttaa atagattgtt ttgatggata agaactaaga
     5101 tgctgtaata cagtaatatt gtgatataac aagattaaaa aataaacata tctatttagt
     5161 ataaatttta aattatagta aactcaaaat ttttttgctt gttttattaa agaatattgt
     5221 tataaggaat gtttgaagga gattttttta ttttgataaa ttttaattat gttagatcgg
     5281 attaaaaaac tttttggaat gaactatact gaaaaagaaa taaacaaatt aaagctggtt
     5341 gttagagaaa taaatcagta ttttcaagaa tatgaaaaat taagtgatga acaaataaag
     5401 gcaaaaactg aagaattcaa aaaaagagtt caagaaaaat gagaaagttt ggatagtatt
     5461 ttgccagaag cttttgctgt ggtaaaacaa gctgctaaaa gaatgtgtgg acaaacagtt
     5521 aatgtaaaat gagaaaaagt tttgcgaaat atgatacctt acgatgttca acttgtttga
     5581 tgaatagttc ttcatcaagg taaaattgct gagatgaaaa cttgagaagg aaaaactttg
     5641 gtagctactt tacctgttta tctaaatgct ttagaatgaa aatgagtgta tgtagttaca
     5701 gtcaacgact atcttgcttc tcgtgatact gaatggatgt gacatttgta tcagcgattg
     5761 tgattaagtg tcggttgtgt ggtaaaatga gtagccattc aagatagaag atgagaatat
     5821 gaaaaagata ttacttatgt agaaaatagt gagcttgggt ttgattatct aagagacaat
     5881 ttagtaaaat ctataaatca aagagtttta actcgaagac ctctaaatta tgcaataata
     5941 gatgagatag attcaatttt aatcgatgag gctaggaccc ctttgattat ttctgaagct
     6001 agagaagaag caacggaaaa gtaccaatat tatgcaaaaa ttgtacaaac acttactcca
     6061 tgtagtggta aaaaaagggt aagtaaatga cttttgtacg aattaatgaa tgaggatgca
     6121 aaaaaagaat ctgtggaaga cggagattat tatatagatg aaaaaacaaa aactgtttct
     6181 ttgagtggag aatgaattca aaaattagaa aaaatgcttg gcgtagaaaa tctctataaa
     6241 gatatatgat acgaggaaat tcatcatata gaaaatgcgt tgagagcaaa agcggtatat
     6301 gaaagagata aagaatatat agtaaaaggt tgagaagttt tgatagtaga tgaacataca
     6361 tgaagaacta tgccaggtag aagatttagc gaatgattac accaggctat tgaggcaaaa
     6421 gaaatggttg aaattaaaag agaatctcaa actatggcaa ccattacata tcaaaatttt
     6481 ttcaaacaat ttggaaagtt gtctggcatg actggtactg ctgtaacaga atgagaagaa
     6541 tttagcaaga tatacaattt agatgttttg gaaatcccta caaataaaga aactataaga
     6601 gtagaccgac acgataaagt atacttcaat caaaatgcca aacgaaaatt tgtaaaggaa
     6661 gatataaaat tttatcatga aatggggcaa ccaatgttga ttggtactgc caacattgct
     6721 acctctgaat atttgagtaa aattttagaa aaggagtgaa tcaatcacta tgttttgaat
     6781 gctaaatttt ttgaacaaga ggctcatatt gtgagtaatg gatgaaaatt taaatctgta
     6841 gtcgtagcta ccaacatggc cggaagatga acagatatta agttggaaaa atgactaaat
     6901 gaaaaattag caaataatta tgtaaaacga gcagaaaaaa atgttaaaaa ctgggatata
     6961 gaatataata tttattcaag taaagaacta gaattaacat tggaggctat aaaacaaatt
     7021 tttggaatag atgatgaagc tattagacaa atgtgaaatg gttgaataga aataaagcaa
     7081 actaaaatta gtaccaaatt taatactagc agaaaaagaa agacagaagt ttttgcaaaa
     7141 atatatttta cactagtttg atgaccgagt ctacccaaca ttacaaaaga cctacaatat
     7201 ggtttgtttg tcttgggtac agaaaaacat gaatctagaa ggatagataa ccaattaaga
     7261 tgaaggtctt gaagacaatg agaccctgga aagtctgttt tttatgtagc attggacgac
     7321 ctaatcatga ggaaaatgtg atgagaaaaa attatgtgaa ttgcaagtat gttaatgaaa
     7381 aaggaagaat tggaaaatct tgagcttact caaaaacaat ttacaagtgc tatagaaaga
     7441 gcgcaaaaac agatagaagc ttggcatttt agtatcagaa aacatctttt tgattatgat
     7501 agtgttatag ataaacaaag gcaaagaatt tataaaaaaa gagatgaaat acttgccagc
     7561 gaattggatg aagaattaaa aaaagagttt gtaaaaaata ccaagaagga tcttagagac
     7621 aatattgatt tgataattaa tgtaaaaatc aatgaagcaa aaaatttaaa acaaactaat
     7681 atagaatttt tggaaacatt gataaaagag ttcaatataa aattagacaa gaaaactgct
     7741 gataaatggg aagcaatggg atttaatgat ttagagctag aaacaattga aactctcgga
     7801 aagtatttag aagaaaaatt aaaaaaactt gatgatgaca aactatatga tatttttaga
     7861 gatgttttgt tgcatcattt agataaacta tgggtagacc atattgatga aatgcaatat
     7921 cttagagata aggtagggtt tatgtgatat gctcaacagg atcctctgat agtctacaaa
     7981 aaagagtctt ttgaaaaatt tcaaagttta atatatacat tcaaaagtac taccactagt
     8041 tatatcttga atttggactt tgatgcaata caaaaacagg atgaaatagc aaaattaatt
     8101 ttggaaaaac aaaagtcagg agataaggag tttttatcta agctatcaaa agttagtgga
     8161 aacctacaag agttaattaa gctggcaaaa caagaagaag taaaaagggc tgaaaacata
     8221 aaaagagaaa tgatttttga agacgaagat ggttttgaag tttttgaagt aggtgatgaa
     8281 aagtgagaaa caaaaaatga tagtgggaaa cctattgaaa ataccaaaat tagaccaaac
     8341 gataaggtta ctgtaaagta taaagatggg aaaatggaat attcaataaa gtacaaaaaa
     8401 gtaaaagatg atgttttagc tggtaagtgt gaggtgatta aaattaatga aaattaataa
     8461 gtgttttatt tttttcaaca aaaaatatgt gaaacttttc aaatctccct ttctgacatt
     8521 atattgggtt tttattggag ataatcatta ctattatcta tgtttcttga attatggtaa
     8581 taataattgg ctttataaaa tcattattta aatttataag aataattttt acaaagaaca
     8641 ccgattcata caaccataaa aatgaaataa gaatgttttt ggggaattat acattacttt
     8701 gactagattt tataataatt gcggatatag tacattctgt aattaatact gaaatacagc
     8761 aactctactc tcttggtatt atagtaataa taagaactgc aatagcatat tttttatgaa
     8821 aagaattaaa agaaattaaa aataagaaat aataatatgt aagtgcaaac aaaaaaaaga
     8881 gagaatgtat aaaataatta taagtgacca aactttttta tctttgtggc attctctcat
     8941 aaaacattat gcacaatata tattttattg aaaagaaaga gtatttatct gttaactttt
     9001 tttaattcgt taatttattc acctcattgt tcatctaaag tcatgccgag aattgcttca
     9061 tctctactta catcatcttt ggaatcttta ttgatataga caaacattgg cataatcatt
     9121 gggactcttc agataatttg cataataaat tctcataaat cttcttttaa aatttttaga
     9181 ttatctttta ctggcatttt tctttttgaa ttttctacat atttagctct agcaaattct
     9241 actacttttg tatggatatc ttttacctca gaagaatata caaaacctct agattctatt
     9301 tgaatgtttc caaccaattc tttgcttttt gcatctacct tgaaaataag agcaagcact
     9361 ccgtcttctg ccataatact acgagccttt attacatatt ccccagacat atgtcatttt
     9421 cattttcaat ccaccatcac agtatctaat ttcaattttt cttttcaaat caaaactaca
     9481 tcatcataca tttcaattat ttctccgttt ttcattggca tcaaaatacg agattctggc
     9541 attcacatat ccattgctaa atttttgtga gcatatcttt cttttgctgg catgaaatac
     9601 ggtaagaaaa attctggtct caataaagat aacattaatt tatggtcttc catacaacca
     9661 tgaccactag catgaacatc aatatcatcg tttgttatta gatggatatc tttgaaaatt
     9721 aaattattta tcattgtgtt tacagatcat tcgttccctg ggattggagt ggcagacatc
     9781 aatattgtat ctcattttct aagttgtaag aaattatgct cgtttctgga cattctagta
     9841 agtgctgcga actcctctcc ctgtgctcca gtagaaagaa ttaaaacttg atcatctgga
     9901 aaagtatcta cctcctcatt tagtttacga atgtatcatt ttggtacttt aatatatcca
     9961 agttgctgac aaatctctat attatttacc atagacctac cagaaagaaa aatagtttta
    10021 tttgctttga tagcactatt tacaatttga ataattcttc aaacattaga tgcaaaagta
    10081 gcaatcaata tccttgtttt tgttttttgt attagctcat ccaaattttc tcatatttct
    10141 ttttctgaaa tagatcgtca ttttttgttt gatcccaagc tatctcaaat ataaagtttc
    10201 actcattccg ctccaatcct agctattttt gctaggtctg ctggtctgtc aatagcagga
    10261 gtatgatcaa ttttgaaatc cgcagaatta aaaattattc atttgggagt atgtatagat
    10321 acagctagtg tttcgggaat attgtggttt actggcacaa attccagagt aaaacatcat
    10381 aattttaaaa tatcaacatc tggatctata attttgtatc ttattttttc tgaatctttt
    10441 ggattttcaa atgttttttt gataatccca agactcaaag gtgtagtata aatcattgga
    10501 tatcaaagtt ctggtagtat atctctcaaa gatcaaacat gatctaagtg accgtgtgta
    10561 ataattattc acctaagttt tttgatgttt tttttaacat agctaatatc tggaataata
    10621 tagtctgctc agaaatttgc atgtgtggta aactccatag cagcatcaat aataatcaga
    10681 tcattttcat attctacaaa catacattgt catacttgtt ctaatcacat taaacttcaa
    10741 attttgactg gtttttctca tttttttatg agtttatgag gatccttaat gtcgtctagt
    10801 tcagtttttc ataaatggcg taaaaaagct tcggatgtca aatccttatc caatgctcaa
    10861 tttacttgtt gattttgtct tcatcttttt tgtttgtttt ttttgctgaa atcaatatta
    10921 agatttcaaa ataagttgtt ttcactcatc attgtttttg tttgtttaaa attaaaataa
    10981 aaattttata tcattgtttt actatcctat gaatctttct ttactcagag aatcttgcat
    11041 agatagaaac attccaataa ttagcataga aactgaagat tttttatcaa aacttttgga
    11101 acagcataag cccaaagttt gtttggaaat aggatgagca gtagcctata gttctatttt
    11161 tatagcaaat aaaatcaaag agcgagatgg aattttatat tcctttgaaa tatcgtataa
    11221 cgactacttg gaaggcatac aaaatgtttg aaagtatgag ttgaaaaatt taactgtata
    11281 tccttttgat tttttgaaaa tagatataaa aaaactaatt ccaaacactt tagattttgt
    11341 gtttgtagat tgacaaaaaa gtcagtatgc taattatcta atgaatatct ttgatatgtt
    11401 gagcaccaaa agtattttgg tgtgtgatga tgtaattaaa tacaaaacta agatgttttg
    11461 attatacgaa tttttagaaa aaaagcaaat aatttatgat gaaattaata cagaggagtg
    11521 agattgagtt ttgttaataa aaaaagaata gttttttctg ttttcgaata aatgttcact
    11581 tatttttatt caatcttgtt tgttcgtaac cgtttcctca tttataacta aaaaagaaat
    11641 tagtcttaaa ttaggggatt ttactcatct tcactttcat caatcattac ttctgcatgc
    11701 tcactttctt tttcttctat tcttaaattt cagtcctcaa gatttaattt aaattttttt
    11761 cattcaagtt ctagttgttc aagttttttt tctacagcat cgtctaagta tagccaagca
    11821 tccaaagatc ttacatcgtc ttcttctaaa attttggctg attgaacgag tcctattgtt
    11881 ttattattta ttgtaataat gtaatttaat ttcccatctt ttgatcaaaa agatctaata
    11941 aataccccat caatatcata ttcccctggg tactctacga tagaataagg caatttttct
    12001 atcactatat cttctcaaat ttctgttgct aaattaatat attctttatt tggaatactc
    12061 gctccaaaaa gaattttgtt ttcaattaaa tatcattcag attctagttt aatattcacc
    12121 attgtaataa ttttataaaa ttaaaaggtt attattatcc aaagaaactt ggatttatta
    12181 gttttaatat tagcccagat gctcaaagta gtatcaatca aaccacaact ttttgtattc
    12241 gttccattcc ttttttataa tttgattcac tatatactca ggtagtcatc atcaatcaag
    12301 ctacaattat gattaaaaat gaaaatatta aaataatagt aactaaaatc ttactcaggc
    12361 ccatcattaa aaaaggaaac gcatttagtt gattaatcgt agttgtgttg ggagcatttg
    12421 atgatcaagg gtcatcttga cttgtcatct ctatacaatc tccaataaat gggactactg
    12481 tatttaacag tattccacaa cactctctgc ttgggtcgca ctcacattta caagtattgc
    12541 tatttaattt ttttcattta tttatacagt catcttctga taaagtacat gtacagctat
    12601 tttcatttgc actccgttgt ttattttgac cacaattgac acagcatcca tcattatcat
    12661 cataccatca ttctcaataa gattcttcac aagattttga actttctggg atcttatcac
    12721 atcaagcaca aaaaggtaac gactcttctt ctaaactttg ttcgcatgta tattcattta
    12781 acttacttaa atctgttgct caaacctcta atcagaaagt gtctgaatta gtacattgtc
    12841 cccaattttt tttaatttga cattcaagtc cagcttctac tttttcacag cataagttgt
    12901 ctaaaattgg ctcatatgat acaggacatg atacctgtgg actaattgaa gttggttctt
    12961 ttcgatctgg gtcaatagct gaacagatat tacagaaaag aaaatctcat atagttacaa
    13021 ctcataattt acaatctcta tgatcttgac aatcggcctc tttcattaag ttttgtaacc
    13081 cccaaacttt ttttgcagaa tcaaatcatc gttcaaattg gtaacttcta tggatcttat
    13141 actttcataa aagggtttct tgataagttt ttaattcatc taaattgttt gtacatagtt
    13201 gttgttttcg ttcaactcta gaagttgcaa gttcaatgtc tgaacaaaca tttggacttc
    13261 atgggtgagt tgttgctaaa cttattgcac ttggactatt aatataccat gcatatatgt
    13321 tattgtttgt agcataactc gtaccactat tggaattatt tgagtagttt ggtatacttg
    13381 aacacatatc agaactcgaa taatcttccc cgtaaacaat agttaatcac aaagaaaaaa
    13441 atcatagaac aaaaatagaa aggcatattc tgatagcgat ttttttcata gattattttt
    13501 ataagtaaaa taataaacta tatattacga tcatttcaaa atttcgttga ttacttcatt
    13561 tgctagagat cattcaacca cttctcaata ttgagatttg atagctcaca tcacttgtca
    13621 tctttgtttt gtaagctccg taatacccaa ttctttgatc aagtttagaa ctatttcttt
    13681 ggtttcctct tttgatttcg tttgtggtaa atattgctct aatacttgtt ttttagcttg
    13741 ttcttctttt aattctttca gcttatttgc ttgtcccaga aatcctattg cttctactaa
    13801 agctttaatt tcttttttca aaattttaat tacttctccg tcttctaaat ctttttgtaa
    13861 ttcaattttt ttatttttta tctgtgcgat tacatagttt aatatcgctt ttttgctaac
    13921 atctttatct ttcatagctt gcatatagtc tgcgaatagt ttttcttgta agctcatttg
    13981 taatttcaaa atataaacga tagtttttat agtaatattc cgttaaaagt ctaatctttt
    14041 ttgctcaaga ggggactcga acccccaagc cttacggcat acgcacctca agcgtatgtg
    14101 tataccaatt ccaccacctg agcattattt taaagtgtgt actcccactg ttcagcggga
    14161 ttctcaatct taagtcaatt actctcactt cgttcgttta attcacttaa tcttggaaca
    14221 attctcccgc tatacagcgg gactttcaat cttgtttttg atttttactc gctttgctcg
    14281 caaaatcaac acaatcttgg agcaccacct gagcattatt ttaaagtgtg tactcccact
    14341 gttcagcggg attctcaatc ttaagtcaat tactctcact tcgttcgttt aattcactta
    14401 atcttggaac aattctcccg ctatacagcg ggactttcaa tcttgttttt gatttttact
    14461 cgctttgctc gcaaaatcaa cacaatcttg gagcaccacc tgagcattaa attcggttaa
    14521 aggtttataa agttacaagt ttataaactt tacgaacttg caactttata aacttaatga
    14581 acttaccatt tctaatatct ataattgctc aataaattca agacaaaaaa attcgcaaga
    14641 ccaaaaattt gtaaaattgg aaatctacat agctctacaa actttttgtt aaatcttatc
    14701 ttactatttt ttttcttctt gctctttttt ttcttttttt actttctttt cttctttttc
    14761 ctttttctcc tctttttcct ttttctcctc ttttttcttt ttttcctctt ttccctcctt
    14821 ttcttctttt tccttctttt cctttttttc cttcttttct tcttttttta attcttgctt
    14881 actattttcc tcttctttta tttttttgat tttttcttca gctttttctt cttccagcat
    14941 tttaactaca tcttccaaac cttttgatgg cttttttatg atttttttgg caatttttcc
    15001 atcctcatct atgcctggag tggaatcttt catactcaat cagatttttc tattttttgg
    15061 atctaataat atcaattttg cttttactac ttctcaaagt tttactactt catttggatt
    15121 ttgtacagat ttttgagata gctcactaag atgaactaat ccattgatat cttcaaatac
    15181 tctgataaat accccataag gaacaaatct cacaacctct cattctataa tatctcatac
    15241 tttgtatttt tttggaagag cagatcgtgg atcttctttt agctttttgc tggataaaga
    15301 gatttttcca ttctccaatc caattacttt tacttgtaat ttatctccaa tttttcaaag
    15361 tctatcaata tttgttacat gtcagtaggt aatttctgaa atatgaacta gtcattctac
    15421 ggtgccccca attgtaacaa aaagtccata agaggatacc cctgaaacaa ttcaatcaaa
    15481 tgtttttcca acggccaatt ccttcaaaac tttatctctt tcttctttta atgcttctct
    15541 ttctgataaa ataattctct tttcttcttc atcaatgttt ataattctta ccgatatttc
    15601 tttcccaatc agctctaata atttatcaaa aatcgcttct tgatctcaat cttctactct
    15661 tggataatga attggagcta attgtgatag aggtataaat catttaattc catgcatatc
    15721 tattaatagt caaccaagat ttgcctctgt aggaacaact ttaataattt catctttttc
    15781 aaattttgct aatatcgatt ttcgaacatc atattgtaat aactttgtta ccgaaacaat
    15841 gtagtatcca tcttcatgtc taatatttgt atttactact tccaattcaa tttctctacc
    15901 aggagccaaa tcataaccac ttctgactaa ctcttttact tcctttgata atattactca
    15961 tgtaaaggct ccattatcac attctaccaa tatgccgttt tctattttct ttaaaataat
    16021 tcatcttatt ttgttacctt ccttgattaa gggtatagaa acttctcaac cttgcagttg
    16081 ttgagtaaac ttttgtaggt ccattatgat ataaaagtta aaaaataaaa attttacttt
    16141 agcttagtta ttataaaaaa attaataaca agcatgaaaa aataatagtc gacaaaacta
    16201 ttatttatta tacctttact caaatttttt tagatacatt tgaataagag tccaagtctt
    16261 gatttttgat gtatttcaaa agtcttcttc tttttgctac cttttttaat aaagatcttt
    16321 tggcatcaaa atcttttgga ttgatttcta tatgtcattg caacatattg atttcttctg
    16381 tcaaagttca aatctgaaat tcaggagatc ctgtgtcatt ttcatgtctt tgaaaaaagt
    16441 tttcgctttt cttttttgcc ataaaattgg tgattaaaaa ataaaataat tgttcaacaa
    16501 actacacaga tgttagccgt tttagaatgt tgagaatata atacaaaaat gtattatggt
    16561 ataaaaatac tcccgacagg actcgaacct gtatctgccc cttaggagag ggctgttcta
    16621 tccagctgaa ctacaggagc gaatttaaca tattaaaaca accaaaaaac tgccgaaaag
    16681 cattctttat atgtctaata ttatgctttc aaaaatcaag aggaaaattt tttgcaagat
    16741 gagctttttg atacaatgtt tttatataaa aattagttga ctttgattaa agttttgtta
    16801 ttataaagct aattttatga ttttattgta taaaatgaat caaaataagt caaaagattc
    16861 aatgcaattt atttatccag ataggtatca agagaagttt ttggataaaa aaaagaaata
    16921 tccatacagt tcaggttgta ttcagagaat tataagtata acattggata cactcaatct
    16981 ccaaaatacc tataaaaatc ttccagttat agttacaaag ctattagaag agccgaaaaa
    17041 cttaactgaa gaggaaaaag aaatgatgag aaaggtaagc gaaaattgag atcttgttag
    17101 agctatacaa aatatctaca ataagaaagt aattttttcc aaaaaagaaa atattataga
    17161 ttttggtgat tacctaaaaa aatatctaga aatcacagga attgattttg atatcttgga
    17221 gctttgcgga caatataatt acatgtccaa atccaaggac gacccaatta ttcttatgga
    17281 tggtgaagca ataagactac aaaaaatcca gactggtcta tctcatggaa aaggaaaagc
    17341 aatttggcgt gataaaaatc gagaaatagt gaaagcaaaa aaaagaaatc aagaattcga
    17401 aattagttgg gttttaattg atagtagagt gaatctgata gataatggtg aaattgaaaa
    17461 aatatatgtt cttgataaga atggaaatga agtggatgtt tattgggata ctaacagaga
    17521 aattgtggaa gtaagtaaat gagaggagag atttgaaatt tgtggaaaca tagaccataa
    17581 tacagtgaga ttagttaatg gtgagaaagt acaaaaagta acaatcagaa ataacgaggg
    17641 ggaaaaaaga gatgttttca ttaataaaaa caaagaaatt gtggaaataa aaatctgaga
    17701 tgaaaaattt gaagctactt gattttgtac tgaagattta attggtaatg aaatattaca
    17761 gcacattact gttagaggtg ctaatatttc taatgttttc cttaataagt atatggaacc
    17821 catcaaaata accaaatgaa aggaagagct taccctctct tatgtttatt atgggactga
    17881 aactttaata agtggagagg aagtacaaaa aataaaagca tcagatatgc tatgagatga
    17941 gagagatgtt gtcattaata aaaagaaaaa aattgtggaa gtaagtaaat gaaaggaaaa
    18001 ttttgagatt tgtgattttt atagagaaaa ggaagaaaat ataataaacg gtgaggctgt
    18061 acaaaaagtg aaaattagaa gtaaaaaatg aaatgagaga gatgttgtca ttaataaata
    18121 taaagaaatt gttgaaataa aaatctgaga taaaaagttt gagctttgtg gtattggtca
    18181 aaaaagcatg gatttaatag gtggtataag tttacaaaat gtttttatca ggaataaatt
    18241 agggaaagta gaaaattatt gtctcaatag aaattgagaa gtaataatgt taaataaatg
    18301 aaaggaagaa gttgtgcttc atgcaacaca ggggataaaa catttaggaa atcagaattt
    18361 ggtccaagag gtaactatta gagataatca aagaaaaact cataaggcag ttgttaatga
    18421 aaataattca acttatactt atatagatct gaattgaaaa gaaaaatact ttgattgaga
    18481 ttgtgatgtt ctttttacta cagatctttc aaaaccattg tttcaaaaag gaataactgc
    18541 agaaggagta attattattg ataattaatt tcattattaa ttaacaactt tttttcaaaa
    18601 accaattttt tttaaatttt atgtaaaata ttacctaaaa atcatcacga ttgaatgttc
    18661 cataattttt tgtatattcc attttgtgat aaaagttgtt tatgacttca cttttctact
    18721 acttttcatt tatccataac aataatcttg tccattttca tcacagtaga taacctatgt
    18781 gcaatcacaa tcgtagtttt atttctcata acttcttcca tcgcctcctg aatcaaagcc
    18841 tcagactcgc tatctagtga agatgttgcc tcatccatca cgataatatc tttatttcca
    18901 agtatggctc tagcaattgc gattctctgt ctctctcatc ctgaaagttt cactcatctt
    18961 tctccaacaa gtgtatcata accatatttt aaattagaaa caaaattatg acatttagcc
    19021 atcttggatg ctgctataat ctcttcttca cttgcatctg gacgaccata agcaatattt
    19081 tctttgattg aacggtgaaa aagtattgga tcctgcggta tcatagacat agatgaccta
    19141 agtgaatttt gtgtaacttg agatatatct tgtcaatcaa tattaatttt accactttga
    19201 atatcataaa gtctaaagag taacttcgtg attgtggttt ttcaactacc agattctcaa
    19261 acaaaagcaa ctctctctca cgactttatg tgcaaactaa gatttttgaa aatttgatta
    19321 tctccatagc taaatccaac tttttcaaat ttaatttctc cggagctcaa gtgcaactct
    19381 ttgtcacttt tatcttggat atctggtata gtttcaacta atgctactat ttctgatatt
    19441 tcagtaatac tataaaaaac tcttctcaat gtttgaccaa ttcatcgcat aaaatctaca
    19501 attctaagaa cataagcttg taataagacg atcattcata ttgaaattat tcaatttcat
    19561 cgcatacgaa gtgcaatata catgattcag acctgcaaaa atatagagaa aagtcatgat
    19621 gttcatcgaa ttatcattga tctaaaatac tgagctttct tggatttaaa tacttcatga
    19681 tttaattttg caaactcttt ttcttctcta gattctgagg caaaaaattt aatagtagta
    19741 ttgtttgtaa tgatatctga atataaccct cacaaattac tatctaaagt atttgccctt
    19801 tcttgatatg gttggatata aacatataat ttatattgaa cacaagaaaa taaaataatc
    19861 aaaagtaaaa gtacaagtga aagccaataa ctttggaatc atataatgat taatatcagc
    19921 aaaacattta ctataaaagg tataatatcc caatttacta tatcgctcaa tctttcaaaa
    19981 gctgatactc attttctata tttactcacc aaagaaccag caaaattatc tgtaaaaaac
    20041 tgaaaagatt gtttttggat ttttttcaaa aacttattat ttagccgttc ttggacatcc
    20101 atttggaagg ttatcgtgaa aaaatcatat attctatatg caacaaatga tcacatttga
    20161 atcccaaaaa gaacaaccaa aatgctaata gcatgtgctg aaagttcagt tgaagtaata
    20221 ccactttgag aaataatatc aaatattttt ttataaaata atggtggaac aatagacaat
    20281 caggaacaaa ggatcctaag tataaaagaa aatagtcaat ttcttaaaaa ctgcttgtag
    20341 gtaagaaaaa aatactgtaa agctttttta tagttgtatt tagtttctat tttatctttt
    20401 ttgggattgg tgtttttcat atcttctttt tttaatcttt caataatcct tctcataatt
    20461 tttattttat tagtcggaca ttttttattt tcaatttttg atatatttat attacaacca
    20521 aagtcaacag aattacttat atattcgctc aaaacatcaa ttcgtaaaaa aatctgaaat
    20581 taaacttctt gtgaaacaaa ggaaattttt ggttgttttt taatgagaaa aacataactt
    20641 ataagagtta tttataattt aatataaaat ttatgtgatt ttcattagaa accattgttt
    20701 cttgggcgaa aagaaaatga tttgtatatc caaattctga aatttattga ggacttgcaa
    20761 atgcttggga ttttggtccc tactgatctc tactcaaaaa aaatatttct gatctatgga
    20821 tcaagcattt tgtgcaagaa agagatgata ttgtatttat ggatacagca attatagctc
    20881 atcccactac ttggcaagca agctgacatt tagcttcttt tgctgatgct ttgatagatg
    20941 acaaaaatac tggtcaaaga tttcgtgcag acaaaattat tgaagatttt tttgaaaaaa
    21001 atagagaatg actaaatgat gaagatttag ctaaaaaact atgagtaacc aacttatctc
    21061 ctgaatcttg gggaaatgag tgaatgagag attttattgt aaaaaatatt ccaaacaatc
    21121 ctgaaacttg aaaaccagcc aattggacag aatgtagaaa tttcaattta atgttggata
    21181 ctcatttatg accagtagca aacgacagtt ctaaagtata catgagacct gaaacctgtc
    21241 agtctttatt tacaaatttt aggtctattc tggatagtac tagaatgaga ataccgtttt
    21301 gattagctca gacatgaaaa gcatttagaa atgaaatcac tccaggtcag tttttgtata
    21361 gaacgagaga gtttgaacaa atggaaatag aatattttgt agaaaatgat gagaataaag
    21421 cgatggaaac ctatgaacaa cggaaaaaag actctatgga atatcgaact aaagtgattg
    21481 aagttaatcc tgaaaattta agatttaaag atcacgaaaa gttagctcac tatgctgctg
    21541 ctgcagtaga tatagaatat agatatccac gatgatttgg agaaattcaa ggagtacaca
    21601 gtagaactga ttttgatctc aaacaacatc aagatacaag tggaaaagat atgcaatatt
    21661 ctgatccatt tactgggaaa agatatatcc cacgatgtgt agaggcctct tggtgactag
    21721 gaagatcagt aatggtaact atgttagaat tttatgatga agaaagttta gaaaatggag
    21781 aaagtcgtgt agttgtcaga tttccaaaac agctcgctcc agtgaagttt gctgttttgc
    21841 cattagtaaa aaaagatgaa aaacaagtag aaattgcaga aaagatattc aaagatttaa
    21901 gtaagaaatt taaatgtgaa tatgatgatt gaggcgctat cgggaaaaga tatcgtagac
    21961 aagatgaaat agggactcct ttttgtatta cagtagatcc acaaagtgta gaagattgaa
    22021 ctgtgactat tagagataga gatagcatgc aacaaaaaag ggtaaaaatt gaagaattag
    22081 aatgatttat gggataatca acttgctaaa tattcgcttg tagactggta tctacgacat
    22141 ttgaatcaag aaataatgta agtttaaatc tattgaaaat gatttaatat ttattacaat
    22201 gtatgtattt atatataaat tgtattacaa atgactacac aaacaatttc agttagagtg
    22261 gattcaaatg ttttagaagc tgtaaaaaaa atagctaagc ttaactgact tagtttaagt
    22321 tctgttatta atgtaaaatt gagagaattc gctaacaata aggaattacg cttaatagat
    22381 gatgaagaat ttgaaatcga tttttgagaa aaatgagcac ctattgatga tgttatagaa
    22441 tatttaaaat ctatagatta aaatggttga acaacgaaaa aaggatctag aaaaaatatc
    22501 aaaggcttga agaaatgaaa ttttaaaaat agcatgtctt attagagatt gaagaactgt
    22561 aggtcttgat ataaaaaaaa tacagtgaaa acaaaatttg tatagatgta gatattgaaa
    22621 atatagaatt attttttcta tactatgcaa taaaatacaa gtaattagag tttgaaacag
    22681 atgaaatatt tataagtgac tataagtgga aaaataaatt gtaaaaatat ttaattctaa
    22741 tatgcaatca tgaaagaaat taagctcagt gaagaaaatt acaaagcact attaaagagt
    22801 ttagcaatag gaaactttat ttattgaatt atggctgata ttgtaaatga tttttataaa
    22861 aaagatgcgc atgattctga tgaggtaatc catcaagtct taaaatgttg tgatgatcct
    22921 aaagtaaagg atatttggga tggaaaaaat gtctttactg aagaatatga ggataacatt
    22981 tggaatgaca tacaagagta tgaaaactat gttgtagaag atttaactgg aattgatgtt
    23041 gaagaattaa accagaaaat cgatgaagaa atgggaaagc acatttaata caattggtat
    23101 ttagtataaa aaggagtgta aatggtaatt ttataagaaa taatatctta aaatggaagt
    23161 aaaagaacta tcagatttta ttgatcgaac tttgacaaaa ggaagatatc cagctctaaa
    23221 ttactcaatt agagaaagag tttttgctca aactctaaaa ataagtgaag aagtatgaga
    23281 attaagtgaa caaatatctt gaaagttttg atgacaaaga ttggacaaaa tagataaatt
    23341 ttctgatgaa agattgggag atgagttagc tgatgtgatt ttttctacta tcagattagc
    23401 tagattgtta gacctagatg ttgatgaaat tttatcaaaa aagatgaaaa tactgagaga
    23461 aagatttgaa aaagaaaatt aataagatac atttataatt tctcaaaaaa atgaacaaaa
    23521 agttactttt tattggatta ggaacaattt taattgtatg aacaattaat acaattatag
    23581 acattcaagc aatgctcaat tgcaattgat gttctgcacc atggcaaaca gctttgataa
    23641 gtacaggatt tttgtacatt ataatatgag ccatatactg gtttatctac ttattacttt
    23701 cacactttaa aaaaattaaa aaatactgat aaaaaacaat aattcccaca aaaaacaact
    23761 ttattgaaaa ctttattgaa aattaatatt gacatttctt gtcttaaatg tatatttaag
    23821 atcaatcttt tagattttaa ttttttttat gaataataat tcaattaact taaattatgg
    23881 ttgagaaagg ggcaaaataa atactagtac aagtgaaaaa tctcaacaaa tatacaatat
    23941 caaacaaaat ttgaatgtaa ttagtaaaaa tattagttat ttaactagcc taaacaaaac
    24001 aaataaagat atttttgata gaattaaaaa acaaattttt aatctcgagg tagcaaatga
    24061 tcgcctaact gtggaaataa attcaattaa aaatgaaatt tgcacaatat tattaaacac
    24121 aaaccttgat ttaaaaatag atttacagaa aaaaaaagat ttgattaaac aaaaagaaaa
    24181 tgaaatactg attaatggag acaagcataa gatattatct cagagattag atgctatatc
    24241 taaagaaata gatggtatag aaaatgaaat taatcaagag ataaaagata aaatatttta
    24301 tttatacatg agtcaattag ttaattttag agaggtttct actgatatca ctatcgatga
    24361 tttaacaata aaaccagaat gacttaggga gtttgtaaat tcatatctta atgaagattt
    24421 tccaaaagac cttttggata aagttatttt tttaaaggga gataaaaata cagggaaaca
    24481 ttcagtagtt aaagcgattg ctaatgaact aaatcgccct atatttagaa taaaatatga
    24541 tgactacttt gatgaagaat gactgtatgt tattttttga ttgatcacct cttatcttag
    24601 acaacagaga gaagaaaaaa acgaacaaat taaacagtat aataagttat taaggcaaat
    24661 aaaaaaaata aaatattgaa cacttgcaaa agtcaatatc tataaaattg aagattgaga
    24721 atgaaatagt tatacttttg atatagggac aaaaaagtga aaatcatgat gtttgttaaa
    24781 attaaaaggt tttgtcgata atataaaaca tactatagat tctattgaag attcttgtgt
    24841 actttatgta gacgatctag atcgtataat acagtcatca aaatatgaaa aatgagaatt
    24901 attgtgaccc gttaaaatga taataaatga tattaagagc gagaattatg atgtaatact
    24961 tgttttggct ggaaacggtt tgggaatcaa tcatggtgat tttaaattat gaattgatag
    25021 ggtgttccag tttaattgaa tcgaagaaaa ctatcaaaga ctatttgagg aaatgttaca
    25081 aaaaaaatta aaaaaactaa atataaaaca taatatctga aatatttcat tgaatggttt
    25141 agatagacag tataggaatg ttaaattttt ggataaatta ataaatagag ttttgaaaaa
    25201 caatatctta aattgaaaag tcattactca agaaatttta gaacttgaat taaacgattt
    25261 aattgaatga gaaaagtcct tatacgactg aatttgattg aaataatgtt gtaattttta
    25321 ttttctctgt tattattatt tagaataatt atatatttat tgtaatttaa tttttttgtg
    25381 aaaatgaaaa taatttcaaa aaatagaata gcatatcatg actatagttt cgaaaaaaaa
    25441 tatgaagtcg gaatagtttt gttgtgacat gaagtaaagt cagtgaaaat atgacagata
    25501 aatatcaaag atgcgatagt aagaatacaa aatagagaac tatggatcaa aaacatggac
    25561 attcctttat acaaaaaaac ttctccaatt ttggcacctg gatatcaagc aaaaagagat
    25621 agaaagttat tgataaataa aagagaatta gctaaaatct cagcattaac tgaccaagca
    25681 tgaaatgtaa taattccttt aaaaatctat ataaacgata aatgactaat taagttagaa
    25741 attggtattg ggaaacttat gagaaaaata gaaaaaaagc aaatactaaa ggaaaaagat
    25801 atcaaaagag atatggatag agaaatgaaa aggcggtaaa aaaatgaatt tataaagttg
    25861 aaagcctgct ccgcagtact acgggcagac aggtttgtaa agtaacaagt ttgtaaagtt
    25921 acaagttata aagttattaa tactttatga acttgatgaa ctttataaac tttacgaacc
    25981 ttttaactta agttcctatc taaaaaatat ttttttatga atcatttcta cagctaactt
    26041 tatactatta gaattttttt gtcattttct taggctatgt tctgtataac gaatatgtac
    26101 gggtatttct ttgtatttaa ttttatgttt tttgatctgt tcgtttaatt cattggcata
    26161 gtgcatccca tcggccataa ttttaatctt tttgaaagaa tctatatgta ttaccctata
    26221 tcaattatgg ggatcggata cttttgcatt ataaaaaatc atggtcacca acctagaaat
    26281 aactaatatc atttttctga aaaatggaat attttctgct tttcatcatt gtataaatct
    26341 ggaacccaaa tatgcttcgt actttcacct atccttttga atttcagaaa taaaagtttc
    26401 catgtctttt atgtccattt gaccatctga atcaaatcaa acaaatcgtt ttattttgag
    26461 ctcatcagaa tatttttgaa taaatttata tcaagtttga tttgctgctc accctcctct
    26521 atttattgta tgagataaaa taataaacaa acagtcagga tattgtttct ttttttcatt
    26581 taaaatttct aaactgttat ccttggatca atcatttata aaaaccattt ttttgaatca
    26641 cgcttctata atttcgtcaa ttacagagcc aaccaccttt cactcattat agactctaat
    26701 attaaaaaca aaatcatctt tttctgtttt atttttgaaa ttttttatat tttctttttc
    26761 caattcatat gttttatcta ttgaaagatg tgtaatcaat cttgtaagtt cgtgcttatc
    26821 tttagtatat ttattaaata atgtaacata aaaatatgcc aatgctacta aagaagcata
    26881 tactattaag tctgctcatc ttgctaatcc aaaaaatgtt ccaaatttat ttaataattc
    26941 gttgtttatt gcaaaaacaa ttattgctaa ggatcataat aaaaatacaa agaaatgtag
    27001 aatattcatt cttttgcgtt tgtacaagtc tatcccaaaa aataggaata taattgcaac
    27061 tataatgatg aaaaattgta acaaagacat gagatatggt ttgaaaagtt aaaaagtttg
    27121 taaagttgca agtttataag ctgttccccc ttattaaggg gtactcaaga actttatgaa
    27181 ctttataaag tcaatatact aattaaaaat acaaaatcca gttaaaaaat gtagactcaa
    27241 attgcctata ttttttgtat ttattataat atgttaagaa ttagaattac ctttttgtgt
    27301 aaatctccaa gcatcttcta tcgcttgata catggatcta ctatactctc gtcccaaaac
    27361 ctgttttgct ttttgaacat ttgcaataga tgtatctatg tctccaggtc ttctagaaac
    27421 tattttgtaa ggaatttttt tttcagtaac tttttctact aattcgatca tctcttttac
    27481 agattttcca tcaccagttc aaatgttgaa tacatcaaaa tatcatttat ctggatcaat
    27541 tttattttct aataaatatt catgatattt taaaatttgg ttgaatgcgc tcaaatgagc
    27601 ttctgctaca tccattacat gaatatagtc tctaactcct gtaccatcag gagtatcgta
    27661 atcatctcca aatatttgta catattctat ctctcagttt gctaccttta gaatataagg
    27721 gattaaatta cttggaattc atttaggatc ttctcatatt aatcaagaat gatgcgctca
    27781 tattggattg aaatatctca aatttacaac attaaatcac ttgaaagtag ctaaatcttt
    27841 taacatatat tccataataa gttttgtggt tccataagga ttgtaagtac ctacacggtc
    27901 attttcacta aaaggaggta ataatttagg ggaatcataa acggtagcac tactagagaa
    27961 aacaatattt tttttatcgt acttttgcat aagttttagc aaattgtttg ttcaaattac
    28021 attgttatca tagtataaaa atggatcctg acaactctcc cctactgctt ttttggcagc
    28081 aaaatgtatt actcaatcta tatttggatg atttttgaaa actttctcaa gttcttctaa
    28141 atttctaata tctatattat aaaatttagg tttttttcat gtgattgtct gaattttgtc
    28201 taaaacagaa atgtctgaat tagatagatt gtctacgata ataatatcat aagcttcttc
    28261 caaaaatacc gtagatgtat gagatcaaat atatcccaat catcaagtaa tgagtatcga
    28321 tctactctgc tcttccataa caaaaattaa cagataaaaa aataggtgag gaaaatataa
    28381 ctacttggag accaatttca agtcttaagt cttaaattac tttagaatag gtgttttgaa
    28441 tatcttttat atatctttta ttaagaaatt ttactaattt aactcttcaa ccgaaaaatc
    28501 ttcgtttaat ttaaactctt ttt
//
LOCUS       DAOSID010000005        26052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_2089_length_26052_cov_14.048775, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000005 DAOSID010000000
VERSION     DAOSID010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 26052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26052
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2089_length_26052_cov_14.048775"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..1179)
                     /locus_tag="PK674_00735"
     CDS             complement(343..1179)
                     /locus_tag="PK674_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="MoxR family ATPase"
                     /protein_id="HOG15089.1"
                     /translation="MMKTMDFLASLMLRDAGKIDGISGVLLSGEPGTGKTRWARLFAE
                     SIEAKFLKYQCHEGTGKEELLYDIDVAGVVERLSPHGDGGATNKKEFLSKGLLPLACE
                     YSHQGKVVFLLDELEKARPAVDNFLLDFLQSGEVFDPNLGHFTANASNLLFVATTNEQ
                     RMLSEPLYRRLRRKKFLFPDAEQLLAIVKAIVPEDAKHLGDTKIKFLINMALWYRDQE
                     VVKKITPPEIARLVSDFTVCATDDEKAEVIFSWFSPHEEDWATLLKYNKGGLKYILGM
                     LK"
     gene            complement(1448..1735)
                     /locus_tag="PK674_00740"
     CDS             complement(1448..1735)
                     /locus_tag="PK674_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15090.1"
                     /translation="MNTLDEYHEELNCRHCGKTIQGNYDPGSYYHQGSIIKSRDKLIL
                     HEKNCELNPNGPNKKLFKDIARCKTQTRISECLNCQHYQKKCNGTKNKNQK"
     gene            complement(1843..2349)
                     /locus_tag="PK674_00745"
     CDS             complement(1843..2349)
                     /locus_tag="PK674_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15091.1"
                     /translation="MEKENLNLQNGDFNDKNNSKIKPEANSEVEKTDYNSIESQIDNV
                     GIKTAEELNDSLENKKIEKIYYANIMRRNPNHGGTGYIKIIGATYDVAFPKEYAWDYD
                     YNVYLLITVEHLSDNRLKLTCKDKEIVVGLSYFERRPESIGENIINKILNIVGYEIDN
                     AREIQRYF"
     gene            complement(2682..3053)
                     /locus_tag="PK674_00750"
     CDS             complement(2682..3053)
                     /locus_tag="PK674_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15092.1"
                     /translation="MNKEQLLNTCRILINDMEKELQENDIGEALEDIRQIFSHFKDLE
                     AQIDILSPKEEEQLDEIIATLSDIDIDKIKSSKEIIEIIDTISGLLNDFKSKIIFGKS
                     DEMIDLIQNMKSLLEILAKKI"
     gene            complement(3204..6509)
                     /locus_tag="PK674_00755"
     CDS             complement(3204..6509)
                     /locus_tag="PK674_00755"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HOG15093.1"
                     /translation="MILLDKEIEQQSGIAKKPLANHSLENSLLKTLNQNKKAEHFVGL
                     LRHGLTIANMEKDMRKEADEPLIESEFGPKSSIKKQANNFLQLGIPIQELCIHFDPIV
                     KRDWQTAKIFCEEIGLTFPNEKEMFDTHIMKEKYPHLKLTRALATRKKGDKHNNEIPI
                     QGTKKNEAGAESILESRVRIFDYIEKIKKNIQNNSYKHHIMIGHRTGTNGFLDAKYNR
                     SKIDLIKDMKESEVSQLDYGFLNQTGEFINLTKQPYSKQGEIKVHNENKIFEFDISLY
                     PQQVEEFKSAVSTLNILLGNNIIADTNIMNAYLEEIKENISNDMSMENIFNYIGINSN
                     FIRTYILSFLVDIIISSKDKNIENIVVEYLDDEGVPEKEKCRWMLKLYNNKHEFWKYV
                     LYKLYMKNKNIIANPIISQFIEEDRKTLIPSPDGRLIPKTPIEQEIEKINQTQKYNNI
                     HIDTLLGNNIFLKDFYVTQNLSGEGENLSSNDLIDNILHENNQLFIIQATGGSGKTIL
                     SKYLLMNLSNFSHLQKNNTYSTRVKEVDLNDYSVDTLENILQGPENIFVIDALDEKNP
                     NTVKKLAEISNSDKFKHSNKKLILLGRYVDDDFVYKNDEKTEKKENVVLYQLQEEVAI
                     SDYIQSFTKIIKPEHIQEKFKKELTDIVDVVDSELKTPIIIEMLANIVKRKISVGRGG
                     KVFVDKNGNEKKDITRKDIYDKFFEYLHQREGQKSDNFGEENERLYLNNPFMGEEYDE
                     LRKDFLSFASNNIFLYNQKNLKNGIVISKGDLHAEMLRFLFTDLFDGGMLQTDAYIKY
                     KKHFDVHDMKLEDFLDFMTTKRETHKTYQMINKKIDIFLETLFAYGFFKNEGDNKISF
                     YHKSFLEYFNYINYSHTFTDTQKYIEYIVKIAHFFDDMPEKIAIFLSIPGFEDSNVMI
                     KVLDEFKKNKDKEIRYPHEKEYRANVYNKALLSVFGNRKVSYKDIQSIGKIHDFVKKV
                     FDGKEEDIISSIEKVKMALKDKELIEQFPFNKEGLEQTLIQLNAGLKIIQDIQREIQE
                     GDDNKDIQEHLNELWSLCKKKNFCFTREGEGNFINTMLSGLDTLYRHSLKDILLMLNG
                     DYLAHIKYQQEYK"
     gene            complement(6534..8252)
                     /locus_tag="PK674_00760"
     CDS             complement(6534..8252)
                     /locus_tag="PK674_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15094.1"
                     /translation="MGIENIKKGKEHLFSSCEFVISGHFGFNRFPAGIGHVDSFISIK
                     QKIDSKCYVGIRKTPQKGLSIEYISMYNGERFLEYEKELIKSHYGPGLDLLEKEMNLN
                     KKTHGYEIAVVSESTRGEGLGFTGTIFSLIVTGIGILTGKYKQNILDDYESFENSIHF
                     HEIKTLAHKCTREIKHGNSGPSYYTTLLQTAHPHVYLSDSHNIQKLDDIQFIKEYYQT
                     IPKMLNLKDISSTTLPIRWAMIYTGQRANTNMVESQKKFTRMKNKNYSERFNNQNRDS
                     IHSHLHKIFSTNGYYEIITNAISAMSIKTLYLFSHIYQYGPRREYIYELINHFNTIHS
                     LYNDIEEDSDIVDDFFSACAQKNVSPDMFGMFPIYTSKFGGNYIIIFEDDSDYDILRI
                     IIESMRTKYPHIRIASKYNFDTPPSQGIKIEQNLFASISSENSENVYVMIDNFGEQTI
                     CNYADIEPEKTSGLFLDAVKGKIYFNGEALTSKDIKSQSTTIEIFDLILQAPDFTIKN
                     NQLGPSTFSGQQNQMLGKIVYPLNKFIYNRTDQEFPFESTGSLREFWLKLGKTNIPVT
                     IIKKMT"
     gene            complement(8417..8803)
                     /locus_tag="PK674_00765"
     CDS             complement(8417..8803)
                     /locus_tag="PK674_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15095.1"
                     /translation="MIKFLYTNEFIHDYKKIKKSYPSLGNYNEDKGDFSDFKRALKTN
                     PTNIGGVERINQLGKSVFLPVYKAKKFACKSLKSTTKLRLIYTYDKCSETIQFIQFIE
                     MYAKADKKNENKDRIKTLLSGKKTLS"
     gene            complement(8796..9524)
                     /locus_tag="PK674_00770"
     CDS             complement(8796..9524)
                     /locus_tag="PK674_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF4065 domain-containing protein"
                     /protein_id="HOG15096.1"
                     /translation="MDYLSKKIKTLRNYANLSQAQMANLLKISRVTYANIESGKRDLK
                     KNEIEKIAHIFELSIENLLEKPVEKTKITKNHPLNKMIQTILYILSQCAGKPNVGKIV
                     VNKLLYFADFNHYEKYWTSITGDLYIKMPMGPMPKSIDAVVALMEKAGIIKTITSEFH
                     GYNQQRFIPNERPDLSVFSATELAELDSVITKYSDKTGKWLTEFSHGDMPRKATEKIG
                     DEISYGLAHYRESLYSVAEVTYND"
     gene            9822..10322
                     /locus_tag="PK674_00775"
     CDS             9822..10322
                     /locus_tag="PK674_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15097.1"
                     /translation="MYNGFNVFQLAFFFVVPVLLFLILFVLYQNLYGKDQKKPKLIVL
                     YSKTMIWALSFLFIIISAKIGDDYLDMRQSFQYDELNQQQEYVDLYGCSQKYGNYDYQ
                     GGSSNLTEEQIETCRVEQRERQQKYTEINHKGVMYKSIYRVGVIAILLIIHIILYYSV
                     RNRKEQ"
     gene            10417..12102
                     /gene="gltX"
                     /locus_tag="PK674_00780"
     CDS             10417..12102
                     /gene="gltX"
                     /locus_tag="PK674_00780"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOG15098.1"
                     /translation="MADWKDLADLMFPDVDQTIADLEKKYPPRTNATCSRFAPSPTGF
                     LHIGLVFSALVSWRFVQQYNGTFILRIEDTDQKRKIDGAVESLLNSMKTFGITIDEGN
                     LGANGEDVGNYGPYTQSERKELYHIFAKELVAKGLAYPCWMTNEEIDNIRDQQQKSKI
                     MPGIYGNYSVYRNIDVDGLIQKVKENPDYIVRFRSHGNTQKKIVFEDVLRGKVNMGDN
                     YNDIVLIKSDGLPTYHLAHIVDDYLMRVSHIIRAEEWLTSVPLHLQLFEAFNLPAPKY
                     CHLAQILKIDEETGKKRKISKRKDPEADVEYFFQNAYSTQGILDYLYTIMDSGFEEWQ
                     KANPDKSFLDRDFVLENMNKSGALFDLTKMESVNNEYLSRISTEQLFNETIEWAEKYD
                     TELANLMKEDVDYTKSALDIERHTPKDPKRFTTFKDVDTQLRFFFDSEYEKLIESVKL
                     KIKSGDEEFQKFTEFDMGIVSSFLDEYLGVLDLDLTVEEGFSQLKEIGKKHGFAGNNA
                     EFKEGGYIGKVGDLAMIIRVGLCASTKTPDLFSVMKVMGKSRIEKRLKKFFHL"
     gene            complement(13138..13626)
                     /locus_tag="PK674_00785"
     CDS             complement(13138..13626)
                     /locus_tag="PK674_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15099.1"
                     /translation="MVTIIISVILIWALYSVLFRSTNFVTLFASGLLIGALVGFVVAL
                     LIPPKFEEKVSSFPLEKVQYKTDTGKYTFLEMETIEDEEYYIFYYYGDNGILEQDKFL
                     TKNVSIKPGCDTAKVEFKKFSRDEDATINYFSIGRPADTEVTIYLPSCPVEGIKSKLS
                     LN"
     gene            13858..14511
                     /locus_tag="PK674_00790"
     CDS             13858..14511
                     /locus_tag="PK674_00790"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF009668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="peroxiredoxin"
                     /protein_id="HOG15100.1"
                     /translation="METQNTLPLIGDMAPAFQAQSTAGPINFPKDYKGKRVVLFSHPA
                     DFTPVCTTEFIGFQKAYEDFKAINTELLGYSVDGLQGHIEWLRNIERNFGVKVEFPLL
                     AGSKIAHLYGMLHPNADDSTTVRAVFVINPEGQIVAIMYYPLSNGRSVAEILRLVKAL
                     QTTAKHKRATPENRPNNPIFGDKVIVPPAGTMKDAVTNAQKYENKDWYICVEQNPNK"
     gene            14660..15256
                     /locus_tag="PK674_00795"
     CDS             14660..15256
                     /locus_tag="PK674_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="ribonuclease H family protein"
                     /protein_id="HOG15101.1"
                     /translation="MKYYVVREGLKRGIFTSRDECKNFVVGVKGARYKSFNSLTEAET
                     ALEKGFEPYYTKEKWFEKDIPFEKNSIAVDAACSRNPGITEYQGIDLVSGETIFHTKL
                     GEGTNNIGEFLALVHGLSYLKKNNSDKAIYSDSKRAIKRVSEGKCKTNLKKTPDNAKI
                     FDLIKRAEDWLAKNKFDTKILKRDTEERGEIPADFGRK"
     gene            15386..15796
                     /locus_tag="PK674_00800"
     CDS             15386..15796
                     /locus_tag="PK674_00800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="HIT family protein"
                     /protein_id="HOG15102.1"
                     /translation="MINPNCIFCKIIGGEIPSVKIRENDDFVAILDSFPNRRGMTLVL
                     SKDHYGSDIFQLDDDFLAKYMKATKEVVSILKKGLGVERVGMVIEGLEVNHAHIKLYP
                     FYDGMGFQIGVGSGPQESLENLNKVAEEICDKSL"
     gene            15933..16865
                     /locus_tag="PK674_00805"
     CDS             15933..16865
                     /locus_tag="PK674_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15103.1"
                     /translation="MNKDIKALVMFSGGLDSILAVKILEKQGIDCTAIIFTSPFFSDK
                     KAKIQAEKFGIKLMSVDITDPHFQMLQNPVYGYGKNLNPCIDCHGLMFKIAGEIADQG
                     GFQIIASGEVVGQRPMSQNKSALNSVSKITGRDILRPLSAKLLPETIYEKQGLVDRNQ
                     LLDISGRARKAQLQLAEEFGLQGYEFPGGGCLLTETGYTNKLKSLFQSFPKEILPIDA
                     ELLKYTRLQIFPRGFVMMGKDNETNLKILEIIQKEKKHSNRYLVLKLKNITGPIGLVR
                     NILGEQNYLHDCKALFKEKVGKLKNMENFDFELS"
     gene            complement(17384..17770)
                     /locus_tag="PK674_00810"
     CDS             complement(17384..17770)
                     /locus_tag="PK674_00810"
                     /inference="COORDINATES: protein motif:HMM:NF021612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HOG15104.1"
                     /translation="MKENLPIIVIGIIIGLIIGYLLSRIYFGIKIRGQRKNAVERSKN
                     VLIGQVNEKIAPILPGFPYNYKDLVFIGKGIDYLVFDGLSNGSLREIVFLEIKSGSSG
                     LNRNETMIKKCIENGKIAYDILRIKN"
     gene            17957..19195
                     /gene="serS"
                     /locus_tag="PK674_00815"
     CDS             17957..19195
                     /gene="serS"
                     /locus_tag="PK674_00815"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="serine--tRNA ligase"
                     /protein_id="HOG15105.1"
                     /translation="MIDLNKVRENLEEYKRVCKLKGKNIDVDYILELDASRKAMQAEI
                     DNLKFQQKELASKQDYEGAKALKSQIQEKEENYESNLEKLDSLLLVMPQFIRDDVVEG
                     KDESENSVLEKYGEIPKFFFEPKDHIELMEKHDMIDFARGAKVSGARSYYLKNDGLLL
                     ERALFQFAMDKMVQKYGFTPMGVPHIVDVRSFVGAGFFPGGEEDAYHLEKDDKWLIAT
                     AENSLISYHTDEILDVSELPKKYTALSPAYRREAGSYGKDTKGCYRVHQFNKIEQVIL
                     CKADIDESNSWHQKILSISLEILSDLGLPHQVVGICTGDLGSGKYVQHDIECRMPSRN
                     AYGETHSVSSLLDFQSRRLGIRYRDIDGTIKYVYTLNNTVLASPRILIPIIENYQTED
                     GKIKIPEVLRKYMNDREYIC"
     gene            19414..20730
                     /locus_tag="PK674_00820"
     CDS             19414..20730
                     /locus_tag="PK674_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="GspE/PulE family protein"
                     /protein_id="HOG15106.1"
                     /translation="MYEQTGQYREALESAIMQSDVIKIFDNLLTIAVEEGASDIHIEP
                     LENYCRIRIRIDGVLLELIQYPKTLHENIISKFKIESGQMRPDEKRLPQDARVSSVTL
                     TNKEIDMRANTLPTVWGEKLVMRIVDKSKKIPPLSELGLEGSNEKIIYKHLSFPNGII
                     LNTGPTGSGKTTTLYACLDYINKPEINIITYEDPVENKMEGLNQAQVRSDIGFTFANG
                     LRGGLRQDPDIMMVGEIRDKETLDMAMESAMTGHLVFSTVHTNSAIETISRILNLGAK
                     PYMMAGTFNLVIAQRLARKTCIHCAKEIDIKSDEKRQMAKKIFSNFDKDLLKKEIVSR
                     GITKEQWSRFMIEGKMLKGTGKLDGKQCPICGGTGYKGRIGLFELMDYTDDIKNMLIE
                     GKSNMEIEQYALKSGMIDLSRDGMFKTIKGLTDLDEIYRCVKMKHS"
     gene            20987..21652
                     /locus_tag="PK674_00825"
     CDS             20987..21652
                     /locus_tag="PK674_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15107.1"
                     /translation="MKKFSILIIVIVFCVFSSFSFTQEILSNVTQDVEKIEINDYYGY
                     LSLLVDLHSEIGRGEVSDKQMFFAVGLINDLKYYSNLDIIEYLGHSFDVQRTLDFLLG
                     NIFEVLTQSEFTMHYINNNLMLLKAKKTDCDSLKDISDKNFVLALKDLDIQNMEINLN
                     KSLDYANCSSYNRINYNAQDKILGELDFYRTVLKHKYDYFYRQKSAIIENYPQILYQL
                     KKH"
     gene            21806..22654
                     /gene="lgt"
                     /locus_tag="PK674_00830"
     CDS             21806..22654
                     /gene="lgt"
                     /locus_tag="PK674_00830"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOG15108.1"
                     /translation="MVAFSIGSINVYWYGIFYLIGFLIGYFFLKFIGKKKIFSKYKNL
                     QKLLSNDVESLLIYIILGVLLGGRLGEVFIYEWGYFSKNLLDIFAVWNGGMSFIGGVI
                     GVFVSLLILKKTKKLSNIEFFLLFDTFLVVVPLAIIFGRFGNYLNQEIYGLVVPANYW
                     GMNDFFVNLLTKLNVFHVYNLVDSNLRLNTNFISIFFEGFVLFLILSITFFRNIKKRE
                     IKPGIIVANFLIFYSIFRFFIEYLRVDSQSQYIWLFTKSQRIFLIFIFTGFVFMLNKK
                     IKTIKL"
     gene            complement(22649..23416)
                     /locus_tag="PK674_00835"
     CDS             complement(22649..23416)
                     /locus_tag="PK674_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15109.1"
                     /translation="MQKNNFDIVVKNLSKYKNKLIEIDKVKEIVKKILDNEYSENKAY
                     KMIYHLKNKGHLLNLKKGIFLVKNPEKEYQEQQLLEMFYRCIVKKHCKGFANNKRYIG
                     GIKALELNMTNFDVPEELLIINDNKQALETIMFEKQISFKTYESNGKSLFNFFKKHTK
                     NVYIGKNVFSIANIELAILESLYNPSIVNKGYTEELIKKVIRKNKKTLALNVFEEILK
                     KNKHHSSINRLYKLSLSIEPEFSENLKNIIKKYSYVL"
     gene            23591..23866
                     /locus_tag="PK674_00840"
     CDS             23591..23866
                     /locus_tag="PK674_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15110.1"
                     /translation="MNSQCLVKDTLSSVKSIDLEGVRAEFTELVEGSYDVVLEINSQK
                     FLIRLVNDGDSIQNFEIIASKGFVSDLTNSLIFALRNLVESLQLALY"
     gene            24001..24858
                     /locus_tag="PK674_00845"
     CDS             24001..24858
                     /locus_tag="PK674_00845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HOG15111.1"
                     /translation="MTDAKKIKSVSNLGQITENLEQVSAFKLQKIRKQTENYKQFMKD
                     FLEIFSSVGFDGEPVNDKGKDLVFVISSDNGFSGNSNQRLFKNIAEKYKNNKEALNIY
                     CLGKKSLDYFLKEGFNVVGYTNLPEEFTEQDFIEIYNYVESSISDKIYSSIKVCFNYF
                     KNTLIRTPVTFNLFPFNKRSLDAFIGNMNIDLNFFSLDSNNLMVGSNLQELKQEMNKQ
                     ISGYIIYGAVLQNKIGELTLDMLLMKNIKNNANETVRGLVASFNKIRESLLTQEVSRI
                     MTSKMFVES"
     gene            24883..25086
                     /gene="nrdD"
                     /locus_tag="PK674_00850"
     CDS             24883..25086
                     /gene="nrdD"
                     /locus_tag="PK674_00850"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOG15112.1"
                     /translation="MNSINDKVGQAINRQPVQCYTRVMGYLRPVSCYNLGKKSEFYSR
                     KYYDKIKAYNRHFVAEYTSDAIC"
     gene            25222..25638
                     /locus_tag="PK674_00855"
     CDS             25222..25638
                     /locus_tag="PK674_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOG15113.1"
                     /translation="MGLIGTEKNIIISEYSKKPLNNFEMEDFTVKYHEGNFICGDDIT
                     VFLKIENEKIKSFSYTGNLSIVSLASASFISEFVINSDINEILKRDYDFVSNKGLEVS
                     NRRKRAVVLPVLAIRNAIHQYLDDGISDDFDDLLDA"
     gene            25797..>26052
                     /locus_tag="PK674_00860"
     CDS             25797..>26052
                     /locus_tag="PK674_00860"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="phage holin family protein"
                     /protein_id="HOG15114.1"
                     /translation="MKVLKDLILNILLHGLALFLLNKYLPSWFWVESVQYTVVITFLI
                     LAILFWIINNIIKVVLKILTIPLKYMTFGLFSLVLNMFMLY"
BASE COUNT         8868 a         3264 c         3930 g         9990 t
ORIGIN      
        1 atttttatca attttacttt tttgttaccg aattactaaa cctatacact tcccccagac
       61 gaacacgtct agattttttg gtctcttcat ttttaatgtg caaccagcca ctttcatgac
      121 taatttttac attaaaacga gcacctttcc acaagtgatt gatttcactt atattctggt
      181 catcgcccaa ttttgccaac ctctcgaaga ggcggctata atttttgtcc gccataatct
      241 tgagatttag cgttacgacc gaacttcgtc gcacaagagt aacctgtttt ttcgtatcag
      301 ataccaaaat acgctcagtt ttgtgtcata agaggaccaa ctttacttca acattcctag
      361 gatgtatttg agtccaccct tattatattt tagtaaagtt gcccagtcct cttcgtgagg
      421 agagaaccag gaaaagatga cctctgcttt ctcatcatca gtcgcacaga cggtgaagtc
      481 ggagaccagt ctggcaattt caggaggagt aatttttttc accacctcct ggtctctgta
      541 ccagagagcc atgttgatga gaaatttaat ttttgtatcc cccagatgct ttgcatcttc
      601 tgggacaata gctttcacta ttgccagaag ttgttccgca tcagggaaca agaatttttt
      661 gcgacggagc cgacggtaca atggctcaga taacattctt tgctcgttcg tcgtcgccac
      721 gaagagtaag ttgcttgcat tggcggtgaa atgtcccaaa tttgggtcaa acacctctcc
      781 tgactgcaag aagtctagga gaaagttgtc cacggcagga cgtgcttttt caagctcatc
      841 cagcaaaaaa accactttac cctggtgcga atactcgcag gcaagtggaa gaagtccctt
      901 ggacaagaac tcctttttgt ttgtggcacc accatcgccg tgaggtgata gtctttctac
      961 gacacctgcc acatcaatgt cgtaaagaag ctcttctttc cccgtgccct catgacactg
     1021 gtatttgagg aacttcgctt ctatggactc agcaaataag cgagcccacc tggttttgcc
     1081 agtaccaggt tctccagaga gaagcactcc ggagattcca tcaattttgc ctgcatcccg
     1141 tagcattagg gaggccaaaa aatccattgt tttcatcata atagattttt attctccctg
     1201 acacgcggga cgcgttctaa cccacaatgg gtagagatat atgatgtaaa aattatagac
     1261 catatatttc tacctattat gtgttagcaa taatttatgt tatttttttg ttaaagacca
     1321 ctttttttta tctcagcaga tagtactgta tgccagacta gaaattttat ctaatggtaa
     1381 gaaaaacaaa ccacaggata ttcataacct gacacagagt actatccact gaggatgatg
     1441 atttttttta tttttgattt ttatttttag taccattaca ttttttttgg taatgttgac
     1501 aattcaaaca ttcagatatt cttgtttgag ttttacatct agcgatatcc ttaaataatt
     1561 ttttattagg accatttgga ttgagttcac agtttttttc atgcaaaatt agtttatctc
     1621 tagacttgat tatagatcat tgatggtaat aacttcacgg atcataattt cattgaattg
     1681 ttttcccgca atgtctgcaa ttgagttctt catgatattc atctaaagtg ttcattttat
     1741 aattttttta aaaagtaaaa ttttagtttt ttaacagttt ttttgttttt tgattttagt
     1801 aatgcttctt aaaaaaaagt atagtttaat aagtataaat agttaaaaat atctttgaat
     1861 ttcacgagca ttatctatct cataaccaac aatatttaat attttattaa ttatattttc
     1921 acctatactt tcaggccttc gttcaaaata actaagccca acgactattt ctttgtcttt
     1981 acaggttagt ttaagacgat tatcagaaag gtgttcaacc gtgataagta aataaacatt
     2041 gtaatcataa tcccatgcat attctttagg aaatgcaaca tcatatgtag ctccaattat
     2101 ttttatatat catgtaccac catgatttgg atttctcctc attatatttg cgtagtaaat
     2161 tttttcaatt tttttatttt ctagtgaatc attcaactct tccgcagttt ttatcccaac
     2221 attatcaatt tgactttcaa tggaattata atccgttttt tctacttcag aattagcttc
     2281 gggcttgatt ttagaattat ttttatcatt aaaatcccca ttttgtaaat ttaagttttc
     2341 tttttccatt tttttatttt taaaaagtaa aattttagag ttttaatagt ttttttgatt
     2401 ttttttttgt ttttgatttt tgttgtacat catatctaag aaatacatag atggtgaaat
     2461 aaaatattta agattttaat ttcatacaag actgttgata atactataca aacagtctta
     2521 tactatttat atatgtagtt aggagatatt actccatcaa ggcatctcgt tctcaagaaa
     2581 tacaagagaa gaggcaacta catctgtctt tttattttga cgactacagt ataataaaat
     2641 aatctgtgaa aaatattgac aaagttgtga aaaattttgt tctatatttt tttcgccaat
     2701 atttctagaa gagacttcat gttttgtatt agatctatca tctcatcaga ttttcaaaaa
     2761 atgatttttg atttaaaatc attcaataag cctgatatag tatcgataat ttctataatt
     2821 tcttttgatg atttaatctt atctatatct atatcagata aggtagcgat aatttcatct
     2881 aattgttcct cttctttggg agaaagaata tcgatttgag cttctagatc tttgaagtgg
     2941 gaaaatattt ggcgtatatc ttccaatgct tctcagatgt cgttttcttg aagctctttt
     3001 tccatgtcgt tgataagtat gcgacaagtg ttaagtaatt gttccttgtt cattgtaata
     3061 tcataaaaca aataaaaata gactttaatc ttcgtagata tataaaaaaa accttacgaa
     3121 tttttgatgt cgcagggaat atagtgtttt tatttgtgaa taagacggac aaagttggga
     3181 aaaattatga ttaagcttat tatttattta tactcttgtt gatattttat gtgagcaaga
     3241 taatctcagt ttaacatcaa tagtatatct tttaatgaat gtcggtataa tgtatctaat
     3301 ccagataaca tggtgtttat aaaatttcat tcaccctctc gtgtaaaaca aaaatttttc
     3361 tttttacata gagaccataa ttcatttaaa tgttcttgta tatctttatt atcatcacct
     3421 tcttgtattt ctctttgtat atcttgtatt atttttaatc atgcatttaa ttgtattaag
     3481 gtttgttcta aaccctcttt attgaaaggg aattgttcta ttaattcttt atcttttaat
     3541 gccatcttaa ctttttcaat ggatgaaata atatcttctt ctttaccatc aaaaactttt
     3601 tttacaaaat catgtatttt tcatatactc tgaatatctt tatatgaaac tttcctattt
     3661 ccaaacacgg ataacaacgc cttattgtat acatttgctc gatattcttt ttcgtgtgga
     3721 taacgaattt ctttatcttt attcttctta aattcatcta atactttaat cataacatta
     3781 ctatcttcaa atcatggtat gctaagaaat attgctattt tttctggcat atcatcaaaa
     3841 aaatgagcta tttttactat atattctata tatttttgtg tatctgtaaa tgtgtgtgaa
     3901 tagttaatat aattaaaata ttccaaaaaa cttttgtgat aaaaagatat tttgttgtct
     3961 cattcgtttt taaaaaatcc gtatgcaaat aaagtttcca aaaatatatc aattttctta
     4021 ttaatcattt gatatgtttt gtgtgtttct cgttttgttg tcataaaatc tagaaaatct
     4081 tctaacttca tatcatgtac atcaaaatgt ttcttatatt tgatgtaggc atctgtttgt
     4141 agcatcccac catcgaaaag atcagtaaat aaaaatctta acatctccgc atgtaaatct
     4201 cattttgata taactatacc attttttaga ttcttttgat tatataaaaa aatattgtta
     4261 cttgcaaaag aaagaaaatc tttccttagc tcatcatatt cttctcccat aaatggattg
     4321 ttaagataca atctttcgtt ttcttctcag aaattatcag atttttgacc ttctctttga
     4381 tgaagatact caaaaaattt atcataaata tctttacgag taatatcttt tttttcatta
     4441 ccatttttat ctacaaaaac tttaccacct cttcatacac ttatttttct tttaacgata
     4501 ttagcaagca tttctataat aatgggtgtc ttaagttcac tatctaccac atccactata
     4561 tcagtaagtt cttttttaaa tttttcttgt atatgctcag gtttgattat ttttgtaaaa
     4621 ctctggatat aatccgagat agctacttct tcttgaagtt gatataatac aacattttct
     4681 tttttttctg ttttttcatc atttttatac acaaaatcat catctacata tcttcacaaa
     4741 agtattagtt tcttattgga atgtttaaat ttatccgaat tacttatttc agcaagcttt
     4801 tttactgtat ttggattttt ttcatccaaa gcatctatca caaaaatgtt ttctggtcat
     4861 tgcaatatat tttcaagagt atctacagag taatcattga gatctacctc ttttactcgt
     4921 gtagaatagg tattattttt ttgtagatgt gagaaattag atagattcat gagtaaatat
     4981 ttggataata tagtttttcc tgatcatccc gtggcttgaa taataaataa ttggttattt
     5041 tcatggagaa tattatcaat taaatcattg gaagatagat tttccccttc tcaagaaaga
     5101 ttttgtgtta cataaaaatc tttgagaaaa atgttatttc acaataaggt atcaatatga
     5161 atgttgttgt atttttgtgt ttggtttatt ttttctatct cttgttctat tggggttttt
     5221 ggtatcaatc ttccatcagg ggagggtatt aatgtttttc tatcttcttc tataaattga
     5281 gatataatag gatttgcgat gatattttta tttttcatat ataatttata taatacatat
     5341 ttccaaaatt catgtttatt gttgtataat tttaacatcc atctacattt ttctttttca
     5401 gggactccct catcatcaag atattctaca actatgtttt caatattctt atctttggat
     5461 gatataataa tatcaactaa aaaagataaa atatatgttc tgataaaatt gctattaata
     5521 ccaatataat tgaaaatatt ttccatactc atgtcattgg atatgttttc ttttatttcc
     5581 tctagatatg cattcattat attagtgtct gctataatat tatttcccag taaaatattt
     5641 agtgtagaaa cagcactttt aaattcttct acttgttgtg gatataatga tatatcgaat
     5701 tcaaaaatct tattttcatt atgtacctta atttctcatt gtttactgta tggttgcttt
     5761 gtaagattaa taaactctca tgtttgattt aaaaatccat aatctagttg agatacttca
     5821 ctttctttca tatctttgat aagatcaatt tttgatctat tatattttgc atccaaaaaa
     5881 ccatttgttc ctgtacgatg acctatcatg atatgatgtt tataagaatt attttgtata
     5941 ttttttttga ttttttctat ataatcaaaa atccttaccc ttgactccag tattgattct
     6001 gctcatgctt cgtttttttt tgttcattgg ataggtatct cattattgtg tttatctcat
     6061 ttttttcttg tagctaaagc tcttgttaat tttaggtgtg gatatttttc tttcattata
     6121 tgagtatcaa acatctcttt ttcgttggga aatgttaatc atatttcttc acaaaatatt
     6181 ttagcagttt gccaatccct ttttactata ggatcaaagt ggatacaaag ttcttgtatt
     6241 ggaattcata attgtaaaaa gttatttgct tgttttttaa tgctagattt gggtcaaaat
     6301 tcagactcta ttaacggttc gtcagcttct tttctcatat ctttttccat attagctatg
     6361 gttaatccat ggcgtaataa tcaaacaaaa tgttctgctt ttttattttg atttaatgtt
     6421 tttagtaaag aattttccaa agaatgatta gcaagaggtt tttttgctat tcatgattgt
     6481 tgttctattt ctttgtctag tagtatcatc aaatatgttg taagataaag tatttatgtc
     6541 atttttttta ttatagtcac aggaatattt gtttttccta attttaacca aaattctctc
     6601 aaagatcatg ttgactcgaa agggaattct tggtcagtgc gattataaat aaatttgttt
     6661 agtggataga ctattttccc aagcatttga ttttgttgtc cagaaaatgt agaaggtcat
     6721 aactgattgt tttttatagt aaaatcagga gcttgtaata ttaaatcaaa tatttctatg
     6781 gtagtagatt gagatttaat atcttttgat gttagtgctt cgccgttgaa atatattttt
     6841 ccttttactg catccaaaaa tagtcatgaa gttttttctg gctctatatc agcataatta
     6901 caaattgttt gttctcaaaa attatctatc atcacatata cattttctga attttcagat
     6961 gaaatagatg caaatagatt ttgttctatt ttaattcctt gggaaggtgg tgtatcaaaa
     7021 ttgtatttac ttgctatacg aatatggggg tattttgttc tcatactttc tataataatt
     7081 cttagtatat cataatcgct atcgtcttcg aatattatta tataatttca tccaaattta
     7141 cttgtatata taggaaacat accaaacata tcaggggaga catttttttg agcacaagca
     7201 gaaaaaaaat catcaacaat atcactatct tcttctatat cgttatataa actatggata
     7261 gtattgaagt gatttataag ttcatatata tactctctac gcggtcaata ttgatagata
     7321 tgagaaaata aataaagtgt ttttatactc attgcactaa tagcatttgt tataatttca
     7381 taatatccat tggtagaaaa tattttatgg agatgtgagt gaatactatc ccgattttga
     7441 ttattgaatc gttcgctata atttttattt ttcattctgg taaacttttt ctgactttct
     7501 accatattgg tatttgctct ttgtcatgta tatatcatcg cccaacggat tggtaatgtg
     7561 gtagaagata tatctttaag atttaacatt ttgggtattg tttgataata ttcttttata
     7621 aattgtatat catctagttt ttgtatatta tgtgaatcgc ttagatatac atgaggatgt
     7681 gctgtttgca gtaaggttgt atagtaggaa ggaccagaat tcccgtgttt tatttcccgt
     7741 gtacacttat gagctaatgt ttttatttca tgaaaatgaa tactattttc aaaggactca
     7801 taatcatcta atatgttttg cttgtatttc ccagtcagta taccaatacc agtaacaatt
     7861 agactaaata ttgtacctgt gaaccctaat cattctcctc ttgtactttc tgaaactacc
     7921 gctatttcat atccatgagt ttttttgtta agattcattt ctttttcgag aagatcaagt
     7981 catgggccat agtgactctt tatcaattct ttttcatatt ctaggaaacg ttctcaatta
     8041 tacatagaaa tgtattctat actgagtcat ttttgtggag tttttctgat ccccacataa
     8101 cattttgaat ctattttttg tttaatactt ataaaactat caacatgccc tatcccggca
     8161 ggaaatcggt tgaatcaaaa atgtcagctt attacaaact cacagctaga aaataaatgt
     8221 tcttttcatt tttttatatt ttctattcac atgatattaa aataaaaata aaactactta
     8281 attatacaaa aaaaaatcat ttttgtcaaa tttttgagct tattttcact actgtccaaa
     8341 tagaaaaaat caataacagg aaaatccgtg tgattagcac ggattttttc tgtaaatact
     8401 gtttaatata aactcattaa cttagagttt tttttcaact tagaagtgtt tttattctat
     8461 ccttgttttc atttttctta tcagcttttg catacatttc tataaattgt ataaactgga
     8521 tagtttcact acatttatca tatgtgtaga tgagtctcaa tttagtagtg cttttaagtg
     8581 atttacatgc aaacttttta gctttataaa ctggcaaaaa aacagatttt cataattgat
     8641 ttattctttc tactcctcat atattagtag gatttgtttt tagtgctctt ttaaaatcag
     8701 agaaatctca cttatcttca ttataatttc atagtgaggg atatgatttt tttatttttt
     8761 tataatcatg tataaattcg ttggtataca aaaatttaat cattatatgt gacttctgct
     8821 acagaataaa gagattcacg atagtgagct aatccataac taatttcatc acctattttt
     8881 tctgtagctt ttcgaggcat atcaccatga gaaaattcag ttaaccattt accagttttg
     8941 tcactatatt ttgtaataac gctatctaat tctgcaagtt ctgttgctga gaaaacagat
     9001 aaatctggtc tttcgtttgg gataaatctt tgttgattat atcaatggaa ttcagaagtt
     9061 atggttttaa tgattcatgc cttttccatg agtgcgacaa cagcatcgat agattttggc
     9121 ataggtcaca ttggcatttt tatatagaga tctcccgtaa ttgaggtcca atacttttca
     9181 taatggttga agtcggcgaa atataaaagt ttgtttacca caatctttcc aacatttggt
     9241 tttcctgcgc attgagaaag tatgtacaga atggtctgta tcattttgtt taatggatga
     9301 ttttttgtga ttttggtttt ttcaacaggt ttttctagta aattttcaat agaaagctca
     9361 aaaatatgtg caattttttc tatttcattc tttttaagat ctcttttacc tgattcaata
     9421 ttggcataag ttactctact tatctttaat aaattagcca tttgtgcttg tgataaatta
     9481 gcatagtttc ttagagtttt tatttttttt gaaagataat ccattataat tttgaataga
     9541 taaaatcaaa taaactataa cctatttttt cttatgtaaa ctatattgag gtatgtggtc
     9601 aaacatgagt ataaagattt ttttacaaaa atcaagaaaa ttgtataatt atttatactt
     9661 ttatttaaca ttgttgtttt aaacaaaatg acaaattgaa aaactttaat atttattgaa
     9721 tttttttgaa cataaagaaa tatattttcc tttaattttg tattgtaaaa tcgttagtat
     9781 tgactatatt gttgtcgttt tattctttaa ccaaagatat catgtataat ggatttaatg
     9841 tgtttcaact tgcttttttc tttgttgttc cagtattgtt gtttttgatt ttgtttgtac
     9901 tttatcaaaa cctttattga aaagatcaaa aaaaacctaa acttattgtt ttgtattcta
     9961 agactatgat ttgggcatta agttttcttt ttattattat atcagcaaaa attggagatg
    10021 attatttgga tatgagacaa tcttttcaat atgatgaatt aaatcaacaa caagaatatg
    10081 tagatttgta tggatgtagc caaaagtatg gaaactatga ttatcaatga tgaagttcta
    10141 atttaacaga agaacaaatt gaaacttgtc gtgttgagca gagagagcgt caacaaaaat
    10201 atactgaaat aaaccacaaa ggtgtgatgt ataagtcaat ttaccgtgtt tgagttattg
    10261 ctatattact gattattcac atcatacttt attattctgt tagaaacaga aaagaacaat
    10321 aattgaaaaa tcaaccattt atcgttgaaa ttataactta taaagttatt tattgtattg
    10381 aatatttttt ttttattttt ttagtatatt ggaaatatgg ctgattggaa agatttagcg
    10441 gatttaatgt tcccagatgt tgatcaaact atagctgatt tagagaaaaa atatccacct
    10501 aggacaaatg ctacttgttc tcgtttcgcg cctagtccca ctggattttt gcatattggt
    10561 ttagtttttt cagcacttgt gtcttggaga tttgtacaac aatacaattg aacctttata
    10621 ttgagaattg aagatactga tcaaaaaaga aaaatagatg gagcagtaga gtctttacta
    10681 aattccatga aaactttttg aattactatt gatgaatgaa atctttgagc taattgagaa
    10741 gatgtttgaa attattgacc ttacactcaa tctgaaagaa aagaattata tcatattttt
    10801 gcaaaagaat tagttgctaa atgattggct tatccttgtt ggatgaccaa tgaagaaata
    10861 gataatatta gagatcaaca acagaaatca aaaataatgc cttgaattta tggtaattat
    10921 tctgtttata gaaatattga tgtagattga ttgattcaaa aagtaaaaga aaatccagat
    10981 tatattgtta gatttagatc tcattgaaat actcaaaaga aaatagtttt tgaagatgtt
    11041 ttgagatgaa aggtaaatat gtgagacaat tataatgata ttgttttgat taaatctgat
    11101 ggtttgccta catatcattt ggctcatatt gttgatgatt atttgatgag ggtttctcat
    11161 ataattaggg cagaggagtg gctaacttca gttccattac atttacagct ttttgaagca
    11221 ttcaatttac cagctccaaa atattgtcat ttggcacaaa ttttgaaaat tgatgaagag
    11281 actggtaaaa aaagaaaaat ttcaaaaaga aaagatccag aagctgatgt agaatatttt
    11341 tttcaaaatg cttattctac tcaatgaata ctagattatc tttatactat tatggattcg
    11401 ggttttgaag aatggcaaaa agctaatcca gacaaatctt ttttagatcg tgattttgtc
    11461 ttggaaaata tgaataaatc tggagcactt tttgatttaa caaaaatgga gagtgtaaat
    11521 aatgaatatt tatctagaat ttctacagaa caactattta atgaaacaat tgaatgggct
    11581 gaaaagtacg atacagaact agccaacctg atgaaagaag atgtagatta taccaaatca
    11641 gcacttgata ttgaaagaca tactccaaaa gatccaaaaa gattcacaac attcaaagat
    11701 gttgatactc aattaagatt cttttttgat tcagaatatg aaaaattaat tgaatcagta
    11761 aaattgaaaa taaaaagttg agatgaagaa tttcaaaaat ttacagaatt tgatatgtga
    11821 attgtttcta gtttcttgga tgaatattta tgagttttgg acttggattt gacggtagag
    11881 gagtgatttt ctcagttgaa agaaatttga aagaaacatt gattcgcttg aaataatgct
    11941 gaattcaaag aatgaggata tatttgaaaa gtttgagatt tggcaatgat tattagagtt
    12001 tgactttgtg cttctaccaa aactccggat ttattttcag ttatgaaagt tatgggtaaa
    12061 agtagaattg aaaaaagatt aaaaaaattt tttcatttat aaaagaaaaa tttgtaaaat
    12121 ttaatgaata actaaaataa atttatatta aatttttaaa aatagaattt taatttttta
    12181 tttatagtta atgtataaat tttagaatat tttttaaaaa agttttgatt ttttttcaaa
    12241 atagtatcaa aatagcattt ttttgatgaa aaaaaggaaa ttttgatgta aaatatatta
    12301 ggtaagaact ggtattaaaa aaatatgtaa aaaaagtcct caaaaatttg acaagaattt
    12361 ttttttgatt ataatagaaa tgtacaaatg agttgagtag aaattatttg atgatttggg
    12421 tacgaaatat tcaatgattc atttagtgac tattcaaagc atcttgtact attctaggaa
    12481 gaaggtcata cacatttaat aattaacttt tgaatcttca ataatatgtc gtttcaaaag
    12541 ttggttcata gttgattttc ttctacttga gtatcatgtt tggattttaa gcttttaatt
    12601 tccaaaagaa gttaaaacaa cttagaattt gaatatgact agattatcta tattattttg
    12661 aataaaaatt ttatagaaat ttagattaga atggcattgt tatggttttg ttcatttata
    12721 tctacatcac ttcaagaatc ttgaattttg ttccacaagg atagaataaa aagttttaga
    12781 agtcatagat atagagcgat taccctcaaa agtaattgat tatacagatt aatttaggcc
    12841 aatttagttt gtataacgaa atctagatgg cattctaact caagcagaag agtactaata
    12901 gaacctaatt ctataaatat agtttgtaca aaaataaaaa taaaaaattg gccagatgat
    12961 ttatttacta acttacaaac tatgtagaat gtctatctag tatttaggct ttggtctatt
    13021 tctagattag accaattatt atttgtttac tgactttctt cctttacctt taaaacttgc
    13081 attgagcagg ttttttttgt tattgaaaaa tctagttttg ttgaaaaaaa actgtctcta
    13141 atttagagac agtttgcttt taattcattc aactggacag ctcgggagat aaatagttac
    13201 ctctgtatct gcaggtcttc caatcgaaaa ataattgata gtagcatctt catcacgact
    13261 aaacttctta aattccactt tggctgtatc acagccaggt ttgattgaga cattttttgt
    13321 tagaaatttg tcttgctcca agatgccatt atctccataa taatagaaga tataatactc
    13381 ttcatcttct atggtttcca tttctagaaa agtatatttc ccagtatcag ttttgtactg
    13441 tactttttct aagggaaagg agcttacttt ttcctcaaat ttaggtggaa ttaaaagtgc
    13501 tacaacaaaa cctacaagag ctccaattaa taatcctgaa gcaaataagg ttacaaaatt
    13561 tgtgctacga aaaaggacac tatacaatgc ccatatgaga atgactgaaa taataattgt
    13621 taccataatt ttaatttttt ttggttagta ctatttttta tttacactta aattgtaatg
    13681 atatatatat atgtcaagtc gttttttttg ttgagtttat ttttctagtt gtaaattagt
    13741 gttgtagaga tattaaattt tcaattgaaa taagtcttca atctattata agaattttgt
    13801 tcaacacgag aacgattctc atttagagaa gttttattag ataaatacaa caatattatg
    13861 gaaacacaaa acacattacc tttaattgga gacatggctc cagcttttca agctcaatct
    13921 acagcttgac caatcaactt tccaaaagat tataaaggaa agcgagtagt tttattctca
    13981 catccagcag attttactcc tgtatgcaca acagaattta tagggttcca aaaagcttat
    14041 gaagatttca aagctatcaa tacagaatta ttatgatatt cagtagatgg actacaatga
    14101 cacatagaat ggcttagaaa tattgaaaga aactttggag taaaagtaga attcccatta
    14161 ttagctggat ctaaaattgc acacttatat ggaatgttac atccaaatgc agatgattcg
    14221 actactgtta gagcggtatt tgtaataaat ccagaaggac aaatcgtagc tattatgtat
    14281 tacccacttt caaatggaag aagtgtagca gagattttaa gactggtaaa agcgttacaa
    14341 actactgcta aacacaaaag agcgactcca gaaaatcgac caaacaatcc aatttttgga
    14401 gataaagtga tagttcctcc tgcaggtacc atgaaagatg ctgttacaaa tgctcaaaaa
    14461 tatgagaata aagattggta tatttgtgta gaacaaaatc caaacaaata agatcactat
    14521 tatcaaaaaa ctcctcgaat tttcagaaat ttaggagttt ttttttgttg taatattttt
    14581 ttgaaaaata tttacaaaaa cttattgaat aataaactta tatatttaga atcaaattac
    14641 atttaaatag tcaaaaaaaa tgaaatatta tgttgtccga gaatgattga aaagatgaat
    14701 atttacttca cgagatgaat gtaaaaattt tgttgtttga gtaaagtgag ctagatataa
    14761 atctttcaat agtttgacag aagctgaaac tgcattagaa aagtgatttg aaccctatta
    14821 taccaaagaa aagtggtttg aaaaagatat tccgtttgaa aaaaatagca ttgcagttga
    14881 tgccgcttgt agtcgcaatc cttgaatcac agaatatcag tgaatagacc ttgttagtgg
    14941 agaaacgatt tttcatacca agctttgaga atgaaccaac aatatttgag aatttcttgc
    15001 tcttgttcac tgattaagtt atttaaaaaa aaacaattca gataaggcga tttattcaga
    15061 ttccaaacga gctatcaagc gagtttcaga atgaaaatgt aaaacaaatc tcaaaaaaac
    15121 accagataat gcaaaaattt ttgatttaat aaaaagagca gaggattggt tggcaaaaaa
    15181 taaatttgat acaaaaatac ttaaacgaga tacggaagaa cggtgagaaa ttccagcaga
    15241 ttttggtaga aagtagaaag ttacaagtta aaagcctgcc ccagccccgt agtcctacgg
    15301 ggcgggtggt aggagagatt attggtttga attttttttt gataaccgta tacatttgaa
    15361 tgttttatat tatattcaaa atatcatgat aaatccaaac tgtatatttt gtaagataat
    15421 ttgatgagaa attcctagcg tgaaaatacg agaaaacgac gattttgtag ctatattaga
    15481 ttcttttcca aatcgtagag gtatgacttt agttttgagt aaagatcatt acggctctga
    15541 tatattccag ttagatgacg attttttggc taaatatatg aaagctacaa aagaagttgt
    15601 ttccatacta aaaaaatgat tgggagtgga gagagtttga atggttattg aatgattaga
    15661 agtaaatcat gctcatataa aattatatcc attttatgat tgaatgtgat ttcaaatagg
    15721 agtatgaagc tgaccacaag aatctctgga aaatttgaat aaagttgccg aagaaatatg
    15781 tgataaaagt ttataatttt aggttttgat aaaaaattgt gttaaaatat gcctgaaata
    15841 aagtatttat taatctaaac attagcttac tatcttaata tataggtacg agcttggatt
    15901 cgtgtctatg gttttataaa atttccgatt tgatgaacaa ggacataaaa gcattagtaa
    15961 tgttttcttg atgacttgat agtattttgg ctgttaaaat attagagaaa cagtgaatag
    16021 attgtacggc gattattttt acaagtccat ttttttctga taaaaaagcg aaaattcagg
    16081 cagaaaaatt tggtataaag ctgatgtcag tagatattac tgatccacac tttcaaatgc
    16141 tacaaaaccc agtttattga tatggaaaaa atttaaatcc ttgcattgat tgtcatggac
    16201 taatgttcaa aatcgcttga gaaatagcag accaagggtg atttcaaatt atagcatcgt
    16261 gagaggttgt gggacaaaga cctatgagcc aaaataaatc agctctaaac tctgtcagta
    16321 aaataacttg aagagatatt cttcgtcctt tgtcagctaa acttttacca gaaactattt
    16381 atgagaaaca aggtttggta gatagaaatc aactactaga tatttcaggt agagctagaa
    16441 aagcacaact tcagttagca gaggagtttg gtttgcaatg atatgaattt ccatgaggag
    16501 gatgtctact taccgaaact tgatatacaa ataaactaaa atctttgttt caatcgttcc
    16561 caaaagaaat attgccaatc gatgctgaac tgttaaagta tactagatta cagatattcc
    16621 cacggtgatt tgtaatgatg tgaaaagata acgaaacaaa cctaaaaatt ttagaaatta
    16681 ttcaaaaaga aaaaaaacat tccaatagat atttggtttt gaaactaaaa aacatcactt
    16741 gaccaatagg tcttgttaga aatattcttt gagagcaaaa ctatttacat gactgtaaag
    16801 ccttattcaa agaaaaagtt ggaaaattaa aaaatatgga aaattttgat tttgagctta
    16861 gttaaaaagt ttgaaaatat aggagtagtt tgttcagatt attaaattaa agtttagaat
    16921 agttctaaaa ttgttatttt taaaaaaact attggtatgc caatagtttt taaatagaga
    16981 gcctcggttt ggttttaaat aaccaattta aataaattac aattttagca attaatttag
    17041 attgataagt taaataggtt ccgaagctct ctataggatt ttatccgacc ttgtttggta
    17101 acagatctgg ataagtttta agaggaatac ctctaacacc ataacccaat tgctaggctt
    17161 aaggacctga tgcagaccct gtgtgataga gcctaagcat aagtcattgt gttaatacaa
    17221 ttatactcaa aaacctttta aatcaaaaaa aacgaagatt ttatttaata ttctcaacca
    17281 tttttttatt tttgtgatat aataaaaaat gcttactgaa agttaagcat tttaagatgt
    17341 ttgattaaga aacatagaag tacacttatc ttgtttaatt tagttaattt ttgattctca
    17401 atatatcata ggcaatctta ccgttttcta tgcatttctt tatcatggtt tcgtttctgt
    17461 ttagtccaga actaccgctt ttaatttcca aaaacactat ttctcttaaa cttcagttgg
    17521 atagtcaatc aaatactaga taatctattc atttacctat aaatactaga tccttgtagt
    17581 tgtatggaaa tcacggtaaa attggtgcga ttttttcatt tacttgtcat atcaaaacat
    17641 ttttagatct ttctactgcg ttttttcttt gtcatcttat ttttattcaa aaataaattc
    17701 ttgataacaa atatcaaatt atcaagccga taattattca aatgacgata attggcaaat
    17761 tttctttcat tttctattgt aaagtaaaaa taaattctta ataatataaa caagtgtttt
    17821 catgtataca ttataaaatg acagtttatt atttcaacac aaaattatgt tttacttgtt
    17881 cgtaggtaga gtctagtata agcataatgt ttttggtgtt tgttttgtaa atttatttta
    17941 ttttttattt aaactgatga ttgatttaaa caaagttcgt gaaaatttag aggagtacaa
    18001 aagagtttgt aaattgaaat gaaaaaatat agatgtggat tatattttag agcttgatgc
    18061 tagtagaaaa gctatgcaag cagagattga taacttaaag tttcaacaaa aagaattagc
    18121 atcaaaacaa gattatgaat gagcaaaagc actaaaatca caaatacaag aaaaagaaga
    18181 aaattatgaa tctaacttgg aaaaattaga ttctttgctt cttgttatgc cccaatttat
    18241 tcgtgatgat gtagtagaat gaaaagatga gtctgaaaat tcagttttgg aaaaatattg
    18301 agaaattcct aaattttttt ttgagccaaa agatcatata gaattgatgg aaaaacatga
    18361 tatgatagat tttgctagat gagcaaaggt ttctggagct agaagttatt atctaaaaaa
    18421 tgatggtttg ttattggaaa gagctttgtt ccaattcgcc atggataaaa tggttcaaaa
    18481 gtactgattt actccaatgg gggtgccaca tattgtggat gttagatctt ttgtttgagc
    18541 aggatttttt cctggaggag aagaagatgc ttatcactta gaaaaagatg ataaatggtt
    18601 gattgctaca gcagaaaact cattgatttc ttatcatact gatgaaatat tagatgtatc
    18661 tgaattgcca aaaaaatata ctgcactttc tccagcttat cgccgtgagg cttgaagcta
    18721 ttgaaaagat acaaaatgat gttatagggt tcatcagttt aataaaatag aacaggttat
    18781 tttatgtaaa gctgatattg atgaatcaaa cagttggcat caaaaaatat taagcatttc
    18841 tttggaaata ctttctgatt tatgactacc tcaccaggtt gtatgaattt gtacatgaga
    18901 tttgggtagt gggaaatatg ttcagcatga tatagaatgt cgaatgccat ctagaaatgc
    18961 gtattgagaa actcactctg ttagttcctt attagatttt caatctagaa gattagggat
    19021 tcgttatcgt gatatagatt gaacaattaa atatgtttat acgctaaata atactgtttt
    19081 agcttctcct aggattttaa ttccaattat tgaaaattat caaactgaag atgggaaaat
    19141 aaaaattcct gaagttttga gaaaatatat gaatgataga gaatatattt gctaataata
    19201 atcggaaaaa ggttataaaa aaggatccat tgcgattctt ttaaggattt tgtaatggtt
    19261 taattgaagt atttgttttg ttaaaattat aaataaattt taaaagtcaa gtcgtattag
    19321 tgttttaatt gttagtattt tagacttttt gttgagtttt gtttataatt gttatacaaa
    19381 aatgattatt ttaagattta ataaaaaatc cctatgtatg aacaaacttg acaatacaga
    19441 gaagcactgg aaagtgcaat tatgcaatct gatgtgataa aaatttttga taatctttta
    19501 actattgcag tagaggagtg agcttctgat atccatatag aaccattgga aaattattgt
    19561 aggattagga ttagaataga tggagttctt ttggagttaa ttcaatatcc aaaaacctta
    19621 catgaaaata ttatttcaaa atttaagata gagtcctgac aaatgaggcc agatgaaaaa
    19681 agacttccac aggatgctag agtttctagt gtaactttga ccaataagga gattgatatg
    19741 agagcaaata ctttgccgac agtatggtga gagaaacttg tgatgagaat tgttgataaa
    19801 tctaaaaaaa ttcctccttt gtcagagctt tgattggaat gaagtaatga aaagataatt
    19861 tataaacatt tgtcatttcc aaatggtatt attttgaata cttgaccaac ttgatctggt
    19921 aaaacaacga cactttatgc ttgtttggat tatataaata aaccagagat aaatattatt
    19981 acatacgaag accctgtaga aaataaaatg gaaggtctca atcaagcaca agtaaggtct
    20041 gatatttgat ttacttttgc taatggtttg agatgatgat taagacagga cccggatata
    20101 atgatggttt gagaaatccg tgataaggaa acattggata tggccatgga gtcagcaatg
    20161 acatgacatt tagtatttag tactgtacat actaattcgg ctatagaaac tattagtaga
    20221 atattgaatt tatgagctaa gccttatatg atggctggaa catttaattt ggttatagct
    20281 caaaggcttg cgagaaaaac ttgtattcat tgtgcaaaag aaatagatat caaatctgac
    20341 gaaaaacggc aaatggctaa aaaaattttc tctaattttg ataaagattt attgaaaaaa
    20401 gaaattgtat caagaggaat tacaaaagaa caatggtcta ggtttatgat agaatgaaaa
    20461 atgttaaaat gaacagggaa acttgatgga aagcagtgtc caatttgtgg aggaacttga
    20521 tataaatgaa gaattggtct atttgaatta atggattata ctgatgatat aaaaaatatg
    20581 ctaattgaat gaaaatcaaa tatggaaatt gaacaatatg ctttgaaaag ctgaatgatt
    20641 gatcttagta gagatgggat gtttaagaca ataaaatgat taacagattt ggatgaaatt
    20701 tatagatgtg tgaaaatgaa acatagttaa gaaaaatgaa attatttttt tatattttta
    20761 tttacttgtt aaagacatct ctttggctta cacggtttac tatattatat aaattatcgt
    20821 gttttagggt tttattcttt aatagttctt ataaaaatat ttattatacc aacttcagtg
    20881 gtatctgggc ttgtaaaaat attagatata tgtatacttt tagggctttt atgtcaaaca
    20941 ccattggcaa atatagtaaa tttgatttaa ttattagttt ttgtatatga agaagtttag
    21001 tattttaata atagttattg tgttttgtgt atttagcagt ttttctttta ctcaagaaat
    21061 tctaagtaat gttactcaag atgtagaaaa aatagaaatt aatgactatt attgatattt
    21121 atcattactt gttgatttac actctgaaat tggtaggtga gaggtttctg ataagcaaat
    21181 gttttttgct gttggtttaa ttaatgattt aaaatattat tctaatttgg atattattga
    21241 atatttgtga cattcttttg atgtacaaag aacactagat tttttactgt gaaatatttt
    21301 tgaagtttta actcaatctg aatttactat gcattatatt aataataatt taatgttact
    21361 caaagctaaa aaaactgact gtgactcctt aaaggatatt tcggataaaa actttgtttt
    21421 ggcactcaaa gatttagata ttcaaaatat ggaaattaat ttgaataaat ctttagatta
    21481 tgcgaattgt tcttcttata atagaattaa ttataatgct caagataaaa ttttatgaga
    21541 attagatttt tatcgtacag tattaaaaca taaatatgat tatttttata ggcaaaaatc
    21601 tgctattata gaaaattatc cgcagatact ttatcaatta aagaagcact aatagtaggt
    21661 ttgtaattct ttaattgttg gtcgttagtt gagggtttat taactagcga ctattaatta
    21721 acgattattt aaatattttt tttcttgaaa ctattgttta tataattatt ctttcttttg
    21781 tatattttaa tttatatttt ttttaatggt cgctttttct attggatcta taaatgttta
    21841 ttggtattga atattttatt taatttgatt tttgatttga tatttttttc ttaaatttat
    21901 tggtaaaaag aaaatatttt ccaagtataa aaatttacag aaactattat caaatgatgt
    21961 ggagagttta ttgatttata ttatactttg agttttattg tgatgaagat tgggagaggt
    22021 ttttatttat gaatggtgat atttttcaaa aaatttatta gatatttttg ctgtatggaa
    22081 tggggggatg tcttttattt gaggagtcat ttgagttttc gtgtcattat tgattcttaa
    22141 aaaaacaaaa aaactatcaa atattgaatt ttttttatta tttgacacct ttttggttgt
    22201 tgttccttta gctattatat ttggaaggtt ttgaaattat ttaaatcaag aaatttatgg
    22261 tcttgtagtt cctgctaact attggtgaat gaatgatttc tttgtgaatc ttcttactaa
    22321 attaaatgtt tttcatgttt ataatttggt tgactctaat ttaagactta atacaaattt
    22381 tatatcgata ttttttgaat gatttgtatt atttttgata ttatcaatta cattttttag
    22441 aaatattaaa aaaagagaga taaagccgtg aatcattgtt gcgaactttt taatatttta
    22501 cagtattttt agatttttta tagagtattt gagagtagat agtcaatcgc aatacatttg
    22561 gctatttaca aaaagccaac gaatattttt gatatttatt tttactggtt ttgtctttat
    22621 gttaaataaa aaaattaaaa caattaaact ataaaacata actatatttt ttgattatat
    22681 tttttaaatt ttctgagaat tctggttcta tactcaaaga tagtttgtat aatctattta
    22741 tactactatg atgtttgttt tttttcaaaa tctcctcgaa cacatttagg gctaatgttt
    22801 ttttattttt tctaattacc ttttttatga gttcttctgt atatcattta tttacgatag
    22861 atggattata taaactttct aatatcgcta attctatgtt ggcaattgaa aatacatttt
    22921 ttcaaatata cacattttta gtatgttttt taaaaaaatt aaataaactt tttcaattag
    22981 attcgtatgt tttgaatgaa atttgttttt caaacataat tgtttctagg gcctgtttat
    23041 tgtcatttat tattagtagt tcttctggaa catcaaagtt agtcatattt aattctaatg
    23101 cttttattca tcatatatat cgtttgttat tggcaaatca tttgcagtgc ttttttacaa
    23161 tacatcgata aaacatttct aaaagttgtt gttcttgata ttctttttct ggatttttta
    23221 ctaagaatat accctttttt aagtttaaaa gatgtcattt gttcttcaaa tgataaatca
    23281 ttttgtatgc tttattttct gagtactcat tatctaatat ttttttaaca atttctttaa
    23341 ctttatctat ttctattaat ttatttttat attttgacaa gtttttaaca acaatgtcga
    23401 aattgttttt ttgcatatcc tacgattaaa gagtaaaatg attaaaatta atataatgtt
    23461 tttacccaaa aaatcaagtg gatttttcca taaaaatttg aaggactttt ctattgtttt
    23521 ttatgtcatt aaagctatta cattcatcga cggatcatta cttaatttta ttttttatgg
    23581 atttgttatt atgaactcac aatgtttagt aaaagacact ctttcaagtg taaaatctat
    23641 tgatttggag tgagtgagag cagaatttac tgaacttgta gaatgaagtt atgatgttgt
    23701 tttggaaatt aattctcaaa agtttttgat tagattggta aatgattgag attctattca
    23761 aaactttgaa attattgcta gtaaatgatt cgttagtgat ttaacaaata gtcttatatt
    23821 tgctcttaga aatttggtag aaagtttgca attagcttta tattaagatc tatttgttag
    23881 tatttgatag tcgttaatag gcttatgata caattttact tttttctaaa aaagacttga
    23941 aaaaggaagt ttttttgtta caacaaaaat tgtaatattt attttctatt aaaaaagcct
    24001 atgactgatg caaaaaaaat caaatctgtt tccaacttag gacagattac tgaaaatttg
    24061 gaacaggttt cagcttttaa actacagaaa ataagaaaac agacagaaaa ttacaaacaa
    24121 tttatgaaag attttttaga aatatttagt tctgtcggtt ttgattgaga acctgtgaat
    24181 gataaatgaa aagatttggt ttttgtgata tctagtgata atggcttttc tggaaactct
    24241 aatcaaaggt tgtttaaaaa tatagcagaa aaatataaaa ataataaaga agcgttgaat
    24301 atttattgtc tttgaaaaaa atctttggat tattttttaa aggaatgatt taatgtagta
    24361 ggatatacaa atttacctga agaatttact gaacaagatt ttatagaaat atacaactat
    24421 gttgaaagtt caatatctga taagatatat tcaagcataa aagtttgttt caattatttt
    24481 aaaaacacac taattagaac gccagtaact tttaatttgt ttccatttaa taaaagaagt
    24541 ttggatgctt ttatttgaaa catgaatatt gatttaaatt tttttagtct cgattccaat
    24601 aatttgatgg tgtgatctaa tttacaagag ctcaagcagg aaatgaacaa acaaatttca
    24661 tgatatataa tctattgagc agttttacaa aataaaatct gagagctgac attagatatg
    24721 ttgcttatga agaatataaa aaataatgct aatgaaactg taagatgatt agtagcatct
    24781 tttaataaga ttcgtgagag tctactaact caagaagtta gtagaattat gacttcaaag
    24841 atgtttgtag aatcttaaat ttattttaat aataataaaa ttatgaactc tattaatgat
    24901 aaggtttgac aagcaattaa caggcaacca gttcagtgtt atacgagagt tatgtgatat
    24961 ttgagaccag ttagttgtta caatctttga aaaaaatctg agttttatag taggaaatat
    25021 tatgataaaa taaaagctta taacagacat tttgttgctg agtatacaag tgatgcgata
    25081 tgctaaaata tttaatctct attccttgtg gggaggatag agtagtttat ttacggttat
    25141 ttaaacataa aacataagaa tcttgaaatt cttatgtttt tttatacatt atatatgttt
    25201 tattttgtag aaagtttttt tatgtgatta atttgaactg aaaaaaatat aataatatct
    25261 gaatattcaa aaaagcctct aaataatttt gaaatggaag attttacagt aaaatatcat
    25321 gaatgaaatt ttatttgttg agatgatata actgtttttt tgaaaataga aaatgaaaaa
    25381 ataaaatcat tttcatatac atgaaacttg tctattgtgt ctttagcgtc tgcttcgttt
    25441 atttctgaat ttgttataaa ttcagatata aacgaaattt tgaaacgaga ttatgacttt
    25501 gtttctaata aagggttgga agtttcaaat agaagaaaga gggcagtagt gttgcctgtg
    25561 ttggctatta gaaatgcgat tcatcagtat ttagatgact gaataagtga tgattttgat
    25621 gacttacttg atgcctaaaa gttttccctc taattatgct gttcgtagag ctagccatca
    25681 acagcaccta ggtggttatg agagaaaggt aatgttgaag tagtagacaa gtttgacttg
    25741 aaatatgtag acaaatgtgt atatagtaaa ttactattta ttagtaattt tttgttttga
    25801 aagtactaaa agaccttata ttaaatatat tattacacgg tttggctttg tttttgttaa
    25861 acaaatattt gccttcttgg ttttgggtag aatctgtaca atatacggtt gtaattactt
    25921 ttttgatact agctattctg ttttggataa taaataatat tattaaggtt gtgttaaaaa
    25981 tattgactat accgctgaaa tatatgactt ttggtctttt ttcattggta ttaaatatgt
    26041 ttatgctata tt
//
LOCUS       DAOSID010000006        25674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_2142_length_25674_cov_16.238417, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000006 DAOSID010000000
VERSION     DAOSID010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 25674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25674
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2142_length_25674_cov_16.238417"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PK674_00865"
     CDS             <1..485
                     /locus_tag="PK674_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=25
                     /product="Hsp70 family protein"
                     /protein_id="HOG15115.1"
                     /translation="ILKVSAEEKSTGKKQDVTIQGATNLSDAEIEKAKEDAEKFAEDD
                     RKRREIVESKNKLEQLTYQMENLLEENKDKLPEEAKEEINKLINASKELKNKEDVTKE
                     EIDKEIEKNQKEFQELYQKYQAEIQNTGKEEVEATGKDKKNDDEKDEAPVDGEVIDAD
                     "
     gene            complement(871..3075)
                     /locus_tag="PK674_00870"
     CDS             complement(871..3075)
                     /locus_tag="PK674_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="SecD/SecF family protein translocase subunit"
                     /protein_id="HOG15116.1"
                     /translation="MKRKLNDKRTIFTALMVLGLIFIFFVGRYFNPETAKRNFIFTTN
                     GISHFRKGLDVSGGTKLVFKISYDKYEEIYKGAELASIKSTIEKIILKNIDNRISALG
                     VSDYKAYVQNMDNNPYIIVEIGGVADLDQAKEIIGKTVELEFRLPKEGEVTEAEKAQR
                     KELVQNLKKDIELSNGQINKFAESRGSQNIFYSNVTGVTLSQLPKLFKNNPRFLSLPK
                     GEISDIITDLFDVVEYQDFSGKIHSDELYGYTFYRINDVKELTRENISLEDIVDISNQ
                     LEYTYEQQIEKSNDLTKDTYDFVGNDLVYNIGPVAENQPAYNVKIIQIATNSPLIGVE
                     SSDLEQLNKERENTIKNVEANIKTKDKFDGGELVVDGRVSELELKSMIPNFDPSIVGD
                     IKTYSQIASDYVVYLIDTKEPNETLFSQLLIKNVNKNHFEEKMKSKLLFDMEIVFVQD
                     RETWKTAQTSKGDILNGAYFKFANTSQSQIGLPVVVINFDDKGKEIFCDITAENLSKQ
                     MAIFIGGEMLTSPTIQSKICGGTAQIDGNFTMDSAKELVDSLNDGALPAPLILMQEEK
                     IAPTLGARALQGALLATLVGILAMYIYMHINYGFKKAMITLGVLVYFLIITAFIIKFI
                     DYALSLSGIAAVILSIGMAIDANILIYERQKEEEARGKTQSSSIDVAYTRSRPAIRDG
                     NISTGIIALFLFMLGSNIFKGFGFMLMITVAITLVFNVPLIKMLLELVYRKK"
     gene            complement(3332..4666)
                     /locus_tag="PK674_00875"
     CDS             complement(3332..4666)
                     /locus_tag="PK674_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15117.1"
                     /translation="MPIEQLEIDHELTDVERNLISNLNLESFDKDGDIDDMTEMQTAL
                     QETKIKVAGEDGGFNEFSLSEIATTFDKYVEKGEGWKLKIKEGLSDTPSRIKDIIENP
                     YNNDLAYLVQKLAELIAYRTATMYTQNKVDLLAKEVVQDEIFGHQTKRTLSGLKNWIE
                     TAENTSNYTTIQNLNTLKDAGSLPEGIVYNEDTGDYEVADGYVRIDPYSSNYAVKKVD
                     TGGVEIYPEPVEGKYYAEFVKNRIETKNPKIQIYGERYDLKITYDRESSSAGGYVYID
                     EIYDGLVFNIGKASTKIPLNELLDKDGTFDDLLREKSLLPKITNTLKENLTALNIAKT
                     IRGKKFKKTDIFPEATDKVFREGKIAKYFDLFTNQEVLIDKGSQYTYADGGNIYIQLD
                     NKASDKHFHKPKKNKDLIIKATELVDENGGLKEEKLKEKMKNIIENIIENHF"
     gene            complement(4701..4904)
                     /locus_tag="PK674_00880"
     CDS             complement(4701..4904)
                     /locus_tag="PK674_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15118.1"
                     /translation="MSNPKKLIREFSKLSYITKLGKVKEILEILAKRSLFFSDLKNHY
                     KNRDDIQENALDAIYAMILNLTH"
     gene            complement(5044..5403)
                     /locus_tag="PK674_00885"
     CDS             complement(5044..5403)
                     /locus_tag="PK674_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15119.1"
                     /translation="MTKQNPDPKAKDQNLNENQASEKKGLGGLMKGFNKFTSKVQEKA
                     KNIGNPVDKIVGGVKNIGNPVDKIVGGVTGFLDKVEKKIEKVSGVDLDSLATMPKKEE
                     KKADEKTDVDVEVKEKK"
     gene            5776..6720
                     /locus_tag="PK674_00890"
     CDS             5776..6720
                     /locus_tag="PK674_00890"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOG15120.1"
                     /translation="MNLLKYNGGGERIDTRLSGQFPYSRNFFHHIISRGGIRVGGKVV
                     KKSYKLKNNDEIFIDDLKRYLSPIILEEAPKIQIPIILEKEDYMVINKPKGVLSHPNS
                     VWEANYPSVVGFLYHNFKKLPSIGNFIRAGLIHRLDKDTDGLMIIAKTEKGLAHFKSL
                     FQEKSEIASSFDENIWNDSEDFDEIDGIDENKIPIKKFYKAKSIITVLGDEFLKKIEK
                     QLPHYIQETVYAKVPNPNPKMGITKILDYKKEGDMVELNLEILTGRTHQIRYHLSNHG
                     LPIAGDYLYGTEENFPMQLTAYRLEFTDPEGEKVHISL"
     gene            6947..7507
                     /locus_tag="PK674_00895"
     CDS             6947..7507
                     /locus_tag="PK674_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15121.1"
                     /translation="MLFDFIAFINELRDNNEKKETIEKYEKLVGPIEGGVKDQVWFVE
                     YLTNFKPTKYLVPEELEKDFDRDLLQQLVLGSFSSDYELKVEKDKQSELYIAVKSGDQ
                     SVVKKVSELWSFQIQRLYEIYIEEQINLQVLKAEDENEKNAIEQERLMRQKRRQAVLD
                     TMGIKEEAEKAKVEQSKSLDELMGKL"
     gene            complement(7581..8207)
                     /locus_tag="PK674_00900"
     CDS             complement(7581..8207)
                     /locus_tag="PK674_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="S24 family peptidase"
                     /protein_id="HOG15122.1"
                     /translation="MARKRELLDDVQRALIAFFKKNTSEEGLTLREIASAIGVNHPQT
                     VLNKLNQLVLKGYFVKDNSGYRLVRESVQSKFDDIIQIPVFGFAQCGNKGKAVIDDYS
                     QEKIPVTLAFIGTSDVQNCFFVRAKGNSMEPKIQDGDLVLIRQQANYDPNDFVFIVHN
                     ELPKLKKIIKEENKFYLESVNRFFDKVELSKYDESKIIGVVKKIIKSV"
     gene            8641..9012
                     /locus_tag="PK674_00905"
     CDS             8641..9012
                     /locus_tag="PK674_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="four helix bundle protein"
                     /protein_id="HOG15123.1"
                     /translation="MNLKFEKLLVRQKAKEIVLNIQKKLKPDDDWGFKSQVQRASISV
                     MDYISQGYEKRYDDAKIKLYLIATSYNNKVLNMISLGKDLGYMAPEVANNILEECINL
                     NKMIYSLVSKIKKRNEVEQTK"
     gene            9217..10269
                     /gene="recA"
                     /locus_tag="PK674_00910"
     CDS             9217..10269
                     /gene="recA"
                     /locus_tag="PK674_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="recombinase RecA"
                     /protein_id="HOG15124.1"
                     /translation="MAKNNENLKEAFKNIEKQFGKGAIMRMGDNSNVGFVNTIHSGSY
                     VLDLILGGGYPEGRIVEIYGPEASGKTTVALHAIAEVQKRGEVAAFIDAEHALDPHYA
                     KKLGVDIDNLLLSQPDHGEQALQIAEELAKTGSVKMIVIDSVAALVPRAEVEGDMGDS
                     YMGLQARMMSQGLRKLTGVLAKTGTICIFINQIRMKIGIMFGNPETTSGGNALKFFAT
                     QRIEIRKGDKLEENKEQIGYFAKIKTAKNKIFAPFKTAEIPVKRGVGYDHIFDIIEAG
                     IILKLITRSGAFYSLGDKKVQGKEKLVELLNEDKKILENLEKDIQKNIKDMRTGKKVL
                     DDDVLDDITEIVENLE"
     gene            complement(10326..10967)
                     /locus_tag="PK674_00915"
     CDS             complement(10326..10967)
                     /locus_tag="PK674_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15125.1"
                     /translation="MNIISTETPISSSEYFEGQNKKSSLKNKLSKNLLHDIQNSEKHS
                     EAKKVAKKVKHIWDLDKIYEAYEDNFANMIARYAIKQIIDHEVKYGQYWYLHDVLLPH
                     PYLNEQEKHRVEENIDKAALNYIKQHTKPFYQTTERFNNQQSVREFQQLVKIIYTDYI
                     SQNVRKKAEKLVLKRVKNKKEHSEFLLFVKNNENLSENMKRTASQSLDEKDKI"
     gene            complement(11002..12468)
                     /locus_tag="PK674_00920"
     CDS             complement(11002..12468)
                     /locus_tag="PK674_00920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG15126.1"
                     /translation="MEKHIYKHKTSHRIYSRIRQINQEFIYLGELLLVCVLGFTGLMM
                     AGTSNLPNNTSFGYPLKQVSTLECRTGYRNDMPNSCKINLPIIHGANYSAYQDVKLYR
                     DIYTILRAASYSDSWNQKIGAHGGVDIATARGTPLYSIGDGEVFFAGRNSAYGNLVRI
                     KYIYKGEIVYAVYAHMDTINVKAGDKVTRGQKIGTVGNTGNASGELGGYHVHFELTKD
                     NFGRPAYAYTNCPDLNKGHYYIIQNGLCRNELMTYQYDPIRIFEAGSTYVPPIENKPV
                     EEQNSTENNEGNKNQNSGVSGTPSTGNESLGRNNTSNSSNTSPSNNTSSDNDGNHGSP
                     SINNEKLNNKPNPTKHNNSNESESIKLEQEPLLIDLDFTGLRDLAEHFSRLWDVEMKS
                     ELKKRSLNLGETVTLDIEVFKKGKSLDRVGNYFNGVLNIPFVFVMNNDNVSININSLQ
                     LITKGKAKVEITGNKVGKTTLIIELDNKKIGYLDIEVK"
     gene            12675..13778
                     /locus_tag="PK674_00925"
     CDS             12675..13778
                     /locus_tag="PK674_00925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG15127.1"
                     /translation="MKKILFVLDYYLPHKGGVENVFENIFLRLEKKGYKIVVLTSHYD
                     KKLKKYENVNGVEIYRTGKSRLSFMCSAIKLGKQILRNNNIDVIHASTYGGAIPASIL
                     GKTFNKRLVLTVHEVFGNLWFKYKGLIAGAIYRLFEWIIFRFKFDVFHCVSQNTANEV
                     KKYYGIKDGKIKVIYNGLDTEFWNPRNVSSKEIFEWRKKHQRNDNFIFLYFGHAGKSK
                     GIDYLIQALPEILKLENVMMVFNIIDSRRTKKILEELKKLDANNLQIFNGFSKKDLRK
                     LVASADCVIAPSISEGFGSVHSEAVAMGKVLITTKTSAIPEVVYGKVKFIKPADKEGI
                     ILACKDVIEGKFENIPEKKFSRDNSVEKIEKLY"
     gene            complement(13883..14674)
                     /locus_tag="PK674_00930"
     CDS             complement(13883..14674)
                     /locus_tag="PK674_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOG15128.1"
                     /translation="MGKIGFFDSGFGGLQTMKYFHKLYPQYDYMFLADNKNCPFGKKS
                     GEEIQKITFNALNRFFDNGAEIVIIACNTAAAHSIRKRQTLYPDKKTLSITVPGVEEI
                     LLSENIGLSIGILATQATITSDIYNDLYYRFGGKQKPDFHFVMAPKLVDMVELGAKEQ
                     EILTEIDRYLAMFPHNLQALVLGCTHFSVYKDYFSQLFSGKIIDPSQNSAIKFIDYLA
                     KHPEIESKLKKNGKVDFYTTGDTKIFDEIGSKIWGKDISSQNMSI"
     gene            14915..16168
                     /gene="murD"
                     /locus_tag="PK674_00935"
     CDS             14915..16168
                     /gene="murD"
                     /locus_tag="PK674_00935"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOG15129.1"
                     /translation="MFVVFGKGKVGSSLKKLLDKLSLENFIMDDQDRNDEILSKSEKI
                     IVSPGIKQNHKLYLKYKNKIFSELNFLGDVVKNLGLSDSLEIIGVTGTNGKSTSVHII
                     YNLFKGIFEKLHIKINIYLAGNFGTPLSETLYEIISGKNENKNLIILETSSFMLYNLN
                     NFNFDYSIITNLGVDHLDWHKDLQEYFDSKFNIVDFTKNYAIMDKDNINLYKKNRTTK
                     AEIEEFQQDFDLNKTNFLGKHNEGNWNASLKIIKKYFEKNNLKWNEEIFWEVAKNIEP
                     LDHRIKMVKEFGGIKIYDDGICTSSQALNVALNSFNEKITLIAGGYDKGDDYTWLANE
                     LEKKVGFACLIGQTAKKFEVILKEKNIDYQIFESLREAVHYAISKAKNLGIKNILFSP
                     GSASFDMFENVYDRCDQFLKIIDIL"
     gene            16520..17989
                     /gene="dnaA"
                     /locus_tag="PK674_00940"
     CDS             16520..17989
                     /gene="dnaA"
                     /locus_tag="PK674_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOG15130.1"
                     /translation="MLIDIVNSYDKEKKEFLLGIWEDVILHLAKQIDPKKIFTFLQKA
                     GIISIDEKEQKVYVGLANEFAMSQVKKFLGKQIKETIKEIYNSQFDTEFVIYTPFSND
                     NELTADLKKLLQLKENKKEHTIEPKIKNKLYEFFGILFDPIYNFENFVVGANNNFAFS
                     AAKAVSENPGTAYNPLFLYGNVGLGKTHLMQAIGNKIMQDDPEKNVIYLPTSKLIDEI
                     VESLKKNKLNTLLSKFNSIDVLLLDDVQFLSGKDKTQEIFHNIFNDFHMNKKQVIISG
                     DRPPKELINIEPRLKSRFGLGLVADIKAPDYETRVAILQSKLEQKGEEIDYNFLEKIA
                     KYVVGNVRELEGALNILITRKKLMGNSLSEQDVTIALETLGYGQQETIDMSNISEGNK
                     KSEKNFSNIVEMVAQYYNVSVADLKSDSRKQEITVARQMLMYLAKTHFGWTLERIGDY
                     FGGKNHTSVIYATNNIEKKIKFDKDLAHDYNVFVEWLEG"
     gene            18063..18989
                     /locus_tag="PK674_00945"
     CDS             18063..18989
                     /locus_tag="PK674_00945"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HOG15131.1"
                     /translation="MFLFKKNSLTLNEIKEKPFKLEKDLQEIAENNLEKVFGLEFVCS
                     EFAVGEFRIDTLAYDPENKSFVIIEYKRDRSFSIVDQGYSYLALLLNNKAEFVLKYNE
                     EFKSPIQKSDINWESSKVIFVANGFTSYQKNAINFKDLPIELWESRQFENEIILFNQV
                     KASKTSESIKTISKGDKTFSKITEEIKTYTIEDHFKEGWNESKEIYENFKNKVLELDS
                     RIEEDPKKWYIAYKVGNKVLIELYVYKSKLNIDFMKIEPKDLKDPEKKVFYKKNSIKH
                     FKRHISTFSINNEDDIDYGIMLIKQVLKKFFL"
     gene            19007..19267
                     /locus_tag="PK674_00950"
     CDS             19007..19267
                     /locus_tag="PK674_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15132.1"
                     /translation="MSQNLEERVLKIEQRNQKVELDKAWETSWTRRFLIAIMTYITIV
                     LFFLVAKLPKPFINSIVPTVGFVLSTLSLPYFKKLWIKKRKK"
     gene            19334..19882
                     /locus_tag="PK674_00955"
     CDS             19334..19882
                     /locus_tag="PK674_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15133.1"
                     /translation="MKKITLFILIGFSVVLLGCKNQGIVNEDWNLIDNNEEELVSDEL
                     EILEDYENIEENNDSTEIIEEKVEEVVEEQVEKQVEDVKVSNTQASKTNTVKEDNLAK
                     CLTSKGIKLYGMAGCPYCKAQIELFGDDFQYINYINCSEDPNVCVEKGISGVPAWEFI
                     DGSIKSGLHSLEDLADLVGCNL"
     gene            complement(19877..20857)
                     /gene="trpS"
                     /locus_tag="PK674_00960"
     CDS             complement(19877..20857)
                     /gene="trpS"
                     /locus_tag="PK674_00960"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOG15134.1"
                     /translation="MRFLTGLKPTGSQLHLGNYFGSIKQMIDLAKANPDSEFFLFLAN
                     MHGFTQIHNQEEMKNNSMMAIKLYLACGADTKQFVIYNPADIPGHAQLNRILTCITHM
                     GFMERMHSYKDALNKGKAKEIGVGVFCYPILMAADILLYDADLVPVGKDQKQHVEYAR
                     DIAQKFNNTFGETFVLPEAKINPELGLIIGTDGRSKMSKSYNNYIGLLDDEATILKKI
                     KQIPTGSQTVEESKNPDECNVYNLCKLFLTETEDKELRAKYLAGGLSYKYAKEYLFEK
                     MMDFLRPIQEKYNKISDQEVKDLLAKNAKYVNEISEKKIKDIYDKVGFNL"
     gene            complement(20884..22509)
                     /locus_tag="PK674_00965"
     CDS             complement(20884..22509)
                     /locus_tag="PK674_00965"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF018158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="CTP synthase"
                     /protein_id="HOG15135.1"
                     /translation="MKLIFVTGGVISGLGKGITAASIGRILKSAGYKVTMAKMDPYLQ
                     IDAGTMSPYEHGEVFVTTDGFETDLDLGHYERFINEELTRDSSMTTGQIYYSVMQKER
                     RGDYLGKTVQVIPHVIDEIKDRVYKLVEGNDVAIIEIGGTVGDIEGPHFIEAMRQLRH
                     DLGKENVLFVHVAPIITISTSGEMKTKAIQHSIIKLRELGIQANVLICRSPVPLENGI
                     KKKLSLFCDLEEDCIIETIDQNIYQVPLSFQEQGLDKYIIERMFGEKKKADMSSWEAT
                     VNKLLNPQREITIALVGKYTQLDDSYLSVLESLKHAGAFYDTKIKIERVDSENYESDF
                     WSDSFRNLINQKNILAVVIPCGFGDRGIEGMINIANFCREKNVPFLGLCLGLQIATIA
                     FARNICNIVDANSSEFKPDGKNNVIDIMPSQKDIENRGGTMRLGSYDAVLKPDTKVRN
                     LYKEFNQIDLSKNLVVERYRHRFAVNPKYHDILQQKGLVVSGTSPDALIVEFIEFPNH
                     KYYVATQAHPEFKSRINKPHPLFLGLIKSVISQ"
     gene            22719..24488
                     /locus_tag="PK674_00970"
     CDS             22719..24488
                     /locus_tag="PK674_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15136.1"
                     /translation="MLNKKRLKLLTLGIILLGSVLFVFAETGKQENIEEEKLAQIMID
                     TGTNTIYISGYVSTGTTSGVIDTGKIEEPPVLINNEPKDLETEFSEALAWMYANGLTK
                     YDNKEDYRMFDLVSREEAGKIIGQAYVALGYPDIEKNSSCDFGDASKFNPSLAPHIAN
                     VCKRGLFKGSNGNYMPLNNLTKAEAMAVLLRMFEGKMSYELQIPWREQYYTKGKLIGL
                     TNIEDINKFDKGLERYEIALMVHRLRSIVTNPQLKTMALNLLGQVLTPNTVGIMDGGT
                     VIANLDTLVGGIDPYKDPELLEAIYRMYDNALTIHKNPNDYRPFDTLNKVAAAKIFDK
                     FSTMLSLSGSEAFLPNQCEFTDIGDLSNEDQQYIVNVCKKGIMKGNNKKFVPDSKLNK
                     SHFVVSLVRMFQGKYLDENVSPWWQNYFNEAKELGIVVPSDAITFDTPISRYEVALFL
                     YKFNIKYKMLSNLNNDRIANEIISTVEGSISTGINGKLKSNVYLDSNLLKRGSFDLGY
                     IETLGTRYKIVKSSESTYFADNFVWYGNVFDLASDEELGTINFVVSNGGYVIEATIRF
                     DGGDTYKILPIQGTSAYYLLQVL"
     gene            24504..25481
                     /gene="ruvB"
                     /locus_tag="PK674_00975"
     CDS             24504..25481
                     /gene="ruvB"
                     /locus_tag="PK674_00975"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF017321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOG15137.1"
                     /translation="MEILDTNTVLVKVKTINIDSVVQDNQENLRPDSFDSFIGQEHIK
                     KILQTAINSAKKRKGSMGHILFCGPSGFGKTTMAHIIAKQSGVNMKVVTGYAINKPAE
                     IVSILNSLEEGDILFIDEIHRLKPNVEEVLYIAMEDFVIDMVMPEGGSVRIPLNKFTL
                     IGATTKGEGLSKPIKNRFVYDFHFMDYNAEEKKIIIDIYLKKYGVPTNKNVIEKIHNK
                     VDSVPREIHNLCIKIRDFFVTEGKNEKEFTQDLRKRFLIHSKIDDGGMSPIHQKYLNI
                     LKRNDRPMGIKSIAIQLGMNEKAVEEDIEPLLIKLGKIEKTIGGRILVS"
BASE COUNT         9323 a         3463 c         3920 g         8968 t
ORIGIN      
        1 gaatcttaaa agtaagtgct gaagagaaat ctactggtaa aaaacaagat gttacaattc
       61 aaggagccac aaatctatct gatgcagaaa tagaaaaagc aaaagaagat gcagaaaaat
      121 ttgctgaaga tgatagaaaa aggagagaaa tagtagaatc caaaaacaaa ctggaacagt
      181 tgacctatca aatggaaaat cttttggaag aaaataagga caaactgcca gaggaagcaa
      241 aagaagaaat aaataaactg ataaatgcta gtaaagaatt aaaaaataaa gaagatgtaa
      301 caaaagaaga gatagataaa gaaatagaaa aaaatcaaaa agaatttcaa gaactttatc
      361 aaaaatatca agctgaaatc caaaatacag ggaaagagga agtagaagct actggtaaag
      421 ataaaaaaaa tgatgatgaa aaagatgaag caccagtaga ttgagaagta atagatgctg
      481 actaaaaccc aacacttgaa tattcctaat attcaataaa tataaaatcc ctcctaactt
      541 aaggagggat ttttcatttc aagcgaaatc taaatccatt ggccttttaa tgcctcttct
      601 tttgcaggcg cgaattcgaa ttcgcgccta cattatttca tcatttcatc actttatcgc
      661 gaacaccgta agcattcatc actttatcat gaacaaagtc cgtatttaaa aactaggtgg
      721 taaccaccat aaattgcagc cccgcagaat tgcggggcta caacattatt atattgtttt
      781 acatttcatc atttcatcat ttcatcattt catcatttta acattttatc attttatcac
      841 tttatcacga acgaagttcg tcaagtagta ctacttcttt ctatatacaa gttccaacaa
      901 catcttaatt aatgggacat tgaaaactag cgtaatagcg actgttatca tcagcataaa
      961 cccaaatcat ttgaaaatat tcgatccaag cataaataga aataaagcta taattccagt
     1021 agaaatattc ccgtctctga tcgctggtcg acttctggta taagcaacat ctatagaaga
     1081 actttgagtt tttcatcttg cttcttcctc tttttgtctt tcatatatta gaatatttgc
     1141 atctatagcc attcaaatag atagtattac tgctgctatt caagataaag atagcgcata
     1201 atctataaat ttaattatga aggcagtaat tattaggaag tatacgagaa ctcaaagagt
     1261 tatcatggct tttttgaatc agtaatttat atgcatatat atatacattg ctaatattca
     1321 aactaaagta gccaacaaag ctccctgtaa tgctctagca cccaaagtag gagcgatttt
     1381 ttcttcttgc atcaatatta atggagctgg taatgctcaa tcatttagag aatctacaag
     1441 ttcttttgca ctatccatag tgaaatttca atctatttga gcggttcctc cacatatttt
     1501 ggattgaatt gtgggagaag ttaacatctc tcatccaata aatatagcca tttgtttact
     1561 taaattttct gcagtaatat cacaaaatat ttcttttcat ttatcatcaa aatttatcac
     1621 aacaactggt aatcaaattt gagattggct tgtatttgca aatttgaaat aagctccatt
     1681 taaaatatct cacttgcttg tttgagctgt tttccaagtt tctctatctt gtacaaatac
     1741 tatttccata tcgaaaagaa gtttggattt cattttttct tcaaaatgat tcttatttac
     1801 attttttata agtaattgtg aaaatagggt ttcattgggt tcttttgtgt caatcaaata
     1861 tacaacataa tctgatgcaa tttgtgagta tgtttttata tctcatacaa tactaggatc
     1921 aaagtttggt atcattgatt ttagttccaa ttccgaaact cgtccatcca ccaccaattc
     1981 tcatcaatca aatttatctt tggtttttat gtttgcttca acatttttta tagtattttc
     2041 tctttcttta tttaattgtt ctaaatcaga tgactctact ccaatcaagg gactgttagt
     2101 ggcaatttgg attatcttca cattgtatgc tggttgattt tctgctactg gtcaaatatt
     2161 gtatactaaa tcatttcata caaaatcata ggtatctttt gttagatcat ttgatttttc
     2221 gatttgttgt tcgtatgtat actcaagctg atttgaaata tctactatat cttccaatga
     2281 tatattttct ctagtgagct cttttacatc atttatacga taaaaggtgt aaccatataa
     2341 ctcatcacta tgtattttcc cactaaaatc ttgatattct acaacatcaa ataaatcggt
     2401 aataatatcc gaaatttctc atttgggtaa actcaaaaac cttggattgt ttttgaaaag
     2461 ttttggtaat tgacttagag taacacctgt cacattactg tagaatatgt tttgagatcc
     2521 tctggattct gcgaacttat taatctgtca attggataat tcaatatctt tttttagatt
     2581 ttgaactaat tcttttcttt gagctttttc tgcttcagtt acctctcatt cttttggtaa
     2641 cctaaactcc aattctacag tttttccaat aatttctttg gcctgatcca aatctgctac
     2701 tcatcaaatc tccactatga tgtatggatt attgtccata ttttgaacat aagctttgta
     2761 atcacttact cccaaagcgg aaattctatt gtcgatattt tttaatataa ttttttcgat
     2821 agtagatttg atggatgcca attctgctcc tttataaatt tcttcatatt tatcataaga
     2881 aattttgaaa acaagttttg ttcatcagct cacatccaat cattttctaa aatgagaaat
     2941 tcaatttgtg gtgaaaatga aatttctttt tgctgtttct ggattgaaat atctacccac
     3001 aaaaaaaata aatattaatc caagaaccat aagagctgtg aatatagtcc tcttgtcgtt
     3061 taattttcgc ttcatctgag aatttaaaaa aaattaaaaa aatatgtgaa atattgaaca
     3121 ataccatatt tttttagcat aaaattgcaa gattaaaaaa acagattcga gatccgtttc
     3181 taaataatgt tactgacttt tttgtacaac ttttctgtgc atcaaccgtc ttttttaagt
     3241 tgtagtttta gagctacacc atactatttt ttggaaaggt gatatagaga ttggttgagc
     3301 taaattacta atcaatgtat gtaaatctaa attaaaaatg attttctatg atattttcga
     3361 taatattttt cattttttct ttaagttttt cttctttaag tcatcaattt tcatccacta
     3421 attcggtcgc cttgattata agatctttat tttttttagg tttgtgaaaa tgtttatcag
     3481 aggctttgtt gtctaattgt atatagatat taccaccatc tgcatatgta tattgggagc
     3541 ccttatcaat aagaacctcc tggtttgtaa aaaggtcaaa gtattttgca atttttcctt
     3601 ctctgaacac cttgtctgtt gcttcaggga aaatatcagt ctttttaaat ttttttcctc
     3661 taattgtttt tgcaatattt aaagctgtta aattttcttt aagagtattt gtgattttag
     3721 gtaagagaga tttttctcga agtaaatcat caaatgtccc atccttatct aaaagttcat
     3781 taagtggaat ttttgttgat gcttttccta tgttgaaaac caaaccatca taaatctcat
     3841 caatatatac ataacctcct gctgaactac tttctcgatc ataagtaatt tttaaatcgt
     3901 atcgttcccc ataaatttga attttgggat ttttagtttc tatcctattt ttaacaaatt
     3961 cagcataata cttaccctca acaggctcag gatagatttc cacaccacct gtatctactt
     4021 ttttaactgc ataattgcta ctatatggat ctatacggac atatcaatca gccacttcat
     4081 aatcaccagt atcttcgtta tatacaattc attcaggtag agaacctgca tcttttagtg
     4141 tatttaaatt ttgtattgtc gtatagttac tagtgttctc agctgtttct atccaatttt
     4201 ttaatcaact taatgttctt tttgtttgat gtcaaaatat ttcatcctgg acaacctcct
     4261 tagctaataa atctacctta ttttgagtat acattgtcgc tgtcctgtat gctataagct
     4321 cagcaagttt ttgcactaaa tatgcaagat cattattgta gggattctct ataatgtctt
     4381 ttatccgact aggtgtatca gaaagtcatt ctttaatttt taatttccat cactctcact
     4441 tttctacata tttatcaaat gtagtagcaa tctcagataa agaaaattca ttaaaccctc
     4501 aatcttctca agctacttta atttttgttt cttgcaaagc tgtttgcatt tctgtcatat
     4561 catctatgtc tcagtcttta tcaaaacttt ctaagttcaa attactgatt aaattgcgtt
     4621 ccacatcggt taattcgtga tctatttcta attgttctat tggcattttt ttaatgttta
     4681 agtaataaaa aactatttta ttagtgagtt aaatttaaaa tcattgcata aatagcatcc
     4741 aaagcatttt cttggatatc atctctattt ttgtaatgat tttttaaatc gctgaaaaat
     4801 agtgatcttt tggcaaggat ttctaaaatt tctttgactt ttccaagctt tgtaatgtaa
     4861 cttaacttgg aaaattctct tataagtttc ttgggattag acattattta catataaata
     4921 tataaaaaaa tctttatcgt agcttaatta taaacaaaaa aagtcctttt gtcaaaaact
     4981 cgaggacttt ttctttattt aaactttaca ttttgtgaaa aaatgttgtg caacacaaga
     5041 tggttatttt ttttctttaa cttccacatc aacatccgtc ttttcatcag cttttttctc
     5101 ttcttttttt ggcatagtag ccagagaatc taaatccact ccagaaactt tttcaatttt
     5161 tttctctact ttatccaaaa aaccagtaac tcctcaaaca attttgtcca cgggatttca
     5221 gatgtttttc actcctcaaa caattttatc cacaggattt cagatgtttt ttgctttttc
     5281 ttgaactttt gaagtaaatt tattaaatca tttcatcaaa cctcacaatc attttttttc
     5341 agaagcttga ttttcattta aattttgatc ctttgctttt ggatctggat tttgttttgt
     5401 catttttttg ttttgtaaaa ggataaaaga tttgtataca atacaagtat aattgttttt
     5461 tgaaaaaatg caaattttac atcccccact atccaagtgc aagcttcgcc ctgcaatact
     5521 caggtctacg gacaggattt tggggattca gactccacta ggtaggaact tttccccaaa
     5581 aaaagttctt ccctttgccc gataaattgg gattcgggaa gttgaacacg aagctagttg
     5641 gaagtacaca aagagttctt cccttgtgaa gcgaagtccg cttccgatag atctcgaaca
     5701 aagtgagatc ccgatatatc gggtcacttt gttcgaaggg gagctataac ttttaaactt
     5761 tataaactta ctatcatgaa tttactgaaa tacaactgag gttgagaaag aatagataca
     5821 cgactaagct gacaattccc gtattcgaga aatttttttc atcacattat tagtagatga
     5881 gggattaggg tttgatgaaa agtagtaaaa aaatcctata aactaaaaaa taatgatgaa
     5941 atttttattg atgatctgaa aagatatctt tctcctatta ttttggaaga agcaccaaaa
     6001 atccaaattc caataatttt ggagaaagaa gattatatgg tcataaacaa gccaaaatga
     6061 gttttatctc atccaaatag tgtttgggaa gccaattatc catctgttgt atgatttctg
     6121 taccataatt tcaaaaagct accaagtatt ggaaatttta ttagggctgg acttattcat
     6181 agattggaca aggatactga cggactaatg attatcgcaa aaaccgaaaa atgacttgct
     6241 catttcaaat ctctttttca agaaaaaagt gaaattgcaa gctcttttga tgaaaatatt
     6301 tggaatgata gcgaagattt tgatgagatt gatggaattg atgaaaacaa aattccaatt
     6361 aaaaaatttt ataaagcaaa atctattatt actgttttgg gagatgagtt tctcaaaaaa
     6421 atagaaaaac aactgcccca ttatattcaa gaaactgttt atgcaaaagt accaaatcca
     6481 aatcccaaaa tgtgaattac aaaaatactg gattataaaa aagagtgaga tatggtagaa
     6541 ttaaatttag aaattttgac tgggagaact catcaaatcc gttaccattt atcaaatcat
     6601 ggattaccaa ttgcttgaga ctatttatac ggaactgaag aaaattttcc tatgcaactt
     6661 actgcttata gattagaatt cacagatcca gagggagaaa aggtacacat aagtttgtaa
     6721 agttatccta ccacctacca cccgggtgca aaataagcaa caccaaccgc agaaagttga
     6781 aagttgaaag ttgcaagttg caagttataa agttattaat actttataaa ctttctaact
     6841 ttatgaactt tctaacttta tgaactttca actttctatt gaaaagtgtg caaattttgg
     6901 tatattcaaa aaaagtatat gttttttata tattaaatta actataatgt tattcgattt
     6961 catagcattt atcaatgaac ttagagataa taacgaaaaa aaggaaacca tagaaaaata
     7021 tgaaaaatta gttggaccaa ttgagggtgg agtaaaagat caagtttggt ttgttgaata
     7081 tttaacgaat ttcaaaccta caaagtatct tgttccagaa gaattggaaa aagactttga
     7141 tcgagattta ttacagcaac tagtgttagg atctttttct agcgattatg aattaaaagt
     7201 agaaaaagat aaacaatctg aattatacat agccgtaaaa agctgagatc aaagtgtagt
     7261 aaaaaaggtt tctgaattat ggtctttcca aattcaaaga ttgtatgaaa tctatataga
     7321 agaacaaata aatttacagg ttttgaaagc tgaagatgaa aatgaaaaaa atgcaataga
     7381 acaagaaaga cttatgagac aaaaaaggcg acaagctgtt ttggacacta tgggtatcaa
     7441 ggaggaagcg gaaaaagcaa aagttgaaca atcaaagagc ttggatgaat taatgtgaaa
     7501 attataaaac caaatagttg aaatataata tataaaaaac ctcaagtaat tgagggtttt
     7561 ttatataaat aattatatta ttacacactt ttaataattt ttttaactac tcaaataatt
     7621 ttgctttcat catattttga taattctacc ttgtcaaaaa atctattcac agattctaga
     7681 taaaatttat tttcttcttt tattattttt tttaattttg gaagttcgtt gtgtactata
     7741 aacacgaagt catttggatc gtagtttgct tgttgacgaa ttaggactag atccccatct
     7801 tgaatttttg gctccatact atttcatttt gctctaacaa aaaagcaatt ttggacatca
     7861 ctagtaccaa taaaagcaag tgtcacaggg attttttctt gggaatagtc atctattaca
     7921 gcctttcatt tatttccaca ttgagcaaaa ccaaaaactg gaatttgtat aatatcgtca
     7981 aatttacttt gaacactttc tctgacaagt ctgtatcaac tattgtcttt gacaaaatat
     8041 cattttaaaa ctaattgatt taatttgttt agaactgttt gtggatgatt aactcaaata
     8101 gctgatgcta tttctctaag agtaagtcac tcttctgatg tgtttttttt gaaaaaagca
     8161 atcaaagctc tttgcacatc atctaataat tcacgctttc tagccattgt tgtttgatta
     8221 gaggataaaa aagtaatttg caaatttata aagttacaaa gttgcaaaac aaattcttcc
     8281 ctcgggggaa gttgggtccc cgcagtattg cggggtagat gggggttgta gttgggataa
     8341 aacccccacc tagctccacg aacaacctcc ccaaggggag gaatttaatc ctcgaacttt
     8401 ataaactcta taatcgtttt ccgatttcag tataaaaatt tctttgacaa agtcaaacct
     8461 tttttgacaa aatcaagtga ttttttgacg attttagatt taccagtctt tacctaatga
     8521 aaaaagtctt gattttttgt tttttttgat taaatattta gtcgaacaaa catctacgaa
     8581 gccactctgt caaacaaaca gacaatggaa tgctctttta catctatata ttatacgaaa
     8641 atgaatttga aatttgaaaa attattagta cgacaaaaag caaaagaaat tgttttaaac
     8701 atccagaaga aattaaaacc agatgatgac tggtgattta aatcacaagt ccaaagagct
     8761 tctatatcag tgatggatta tatatcacaa tgatatgaaa aacgatatga cgatgcaaag
     8821 attaaattat atctaatagc aacttcatat aacaataaag tattaaatat gataagttta
     8881 tgaaaagatt tgtgatatat ggctcctgaa gtggcaaaca atattttgga ggagtgtata
     8941 aatttaaata agatgattta tagtcttgtg tccaaaatca aaaaaagaaa tgaggttgaa
     9001 caaactaagt aagtcaaaag ttataaagct gaaagttgca agtttataaa gttacaaatt
     9061 ataaagttat taatacttta taaactttat gaactttacg aactttacaa actttataaa
     9121 cttaataaac taaattaaag cattcgtggc tttgatgttt gtgggaaata ataggttaaa
     9181 tgttgaaaaa aatgttttaa tcgtaacgaa tataaaatgg caaagaacaa tgaaaacttg
     9241 aaagaggctt tcaagaatat tgaaaagcaa tttggtaaag gagctattat gagaatgtga
     9301 gacaattcaa atgtttgatt tgtaaatact atccatagtg gtagttatgt tttggatcta
     9361 atattatgat gatgatatcc agaatgaaga attgtagaaa tttactgacc agaagcgagt
     9421 tgaaaaacta ctgttgcttt acatgctata gctgaagtac aaaaaagagg agaagtagct
     9481 gcatttatag atgctgaaca tgctttagat cctcattatg ccaaaaaact tggtgtagat
     9541 atagataatt tattattatc tcagcctgat cactgagaac aagcattaca aatagcagaa
     9601 gaattagcaa aaacttgatc tgtaaagatg attgttatag attctgtagc tgctttggtt
     9661 ccaagagcag aagtggaatg agatatgggg gatagttata tgtgattaca agctagaatg
     9721 atgtcgcagt gacttagaaa attgacagga gttttagcaa aaacagggac tatttgtata
     9781 tttataaacc aaataagaat gaaaataggg ataatgtttg gtaatcctga aaccacttct
     9841 gggtgaaatg ctttaaagtt ttttgctact caaagaatag aaattagaaa atgagataag
     9901 ttagaggaaa ataaagaaca aatatgatat tttgctaaaa tcaaaacagc caaaaataaa
     9961 atatttgcac ctttcaaaac cgcagaaatc ccagtaaaac gatgagttgg ttatgatcat
    10021 atctttgata taatagaggc tgggattata ctaaaattga taacaaggtc gtgagctttc
    10081 tattcccttg gagataaaaa agtacaatga aaagaaaaat tagtggaatt gttgaatgaa
    10141 gataaaaaaa tattggaaaa cttggaaaaa gatatacaaa aaaatattaa agatatgaga
    10201 acatgaaaaa aggttttgga cgatgatgtt ttagatgata ttactgaaat tgtagaaaat
    10261 ctagaataaa ataaaaatat ataataaaaa gtcttcgtcg taataatacg gagacttttt
    10321 tttaattata ttttatcttt ttcatccaaa gattgacttg cagttctttt catgttttca
    10381 gaaagatttt cgttattttt tacaaagagg agaaactcag aatgttcttt tttatttttt
    10441 actcttttta atactaattt ttcagctttt tttctaacat tctgtgaaat atagtcagta
    10501 taaattattt tcactaattg ctgaaactca cgaacacttt gttgattatt aaacctttct
    10561 gtggtttggt aaaaaggttt cgtatgttgt ttaatatagt tcaatgctgc cttatctata
    10621 ttctcctcaa ctctatgctt ttcttgttca ttaagatagg gatgaggtaa taatacatca
    10681 tgaagatacc aatactgacc gtactttact tcatgatcaa tgatttgttt aattgcatat
    10741 cttgcaatca tattagcaaa attatcttca tatgcttcat agatcttatc taagtcccaa
    10801 atgtgtttta ctttcttcgc tacttttttt gcttcactat gtttctcact attttgtata
    10861 tcatgtaaaa gattttttga taatttgttt tttaaagaac tttttttgtt ctggccctca
    10921 aaatattccg atgaagaaat cggagtttct gttgatataa tgttcattag tatatattta
    10981 atatataaaa atcagaattt tttattttac ttctatatcc aaatatcaaa tctttttatt
    11041 atccaactct attattaatg ttgtttttca tactttattt ccagttattt ctactttggc
    11101 ttttcatttc gtgattagtt gtaatgagtt gatatttata gatacattat cgttgttcat
    11161 aacaaaaaca aaaggaatgt ttaacactca attaaagtaa tttcaaactc tatccaagct
    11221 ttttcatttt ttgaatactt caatatctaa ggtaacagtt tcgcctaaat taagagatct
    11281 ctttttaagt tctgatttca tttccacatc ccataaacga gagaaatgtt ctgccaagtc
    11341 ccttaatcat gtaaaatcta ggtctattaa caaaggctcc tgttccaact taatactttc
    11401 tgattcgttt gaattattgt gttttgtagg attcggcttg ttgtttagct tttcattatt
    11461 tatggatgga gaaccatgat ttcaatcatt atctgaggaa gtattatttg aaggactggt
    11521 atttgatgaa ttgcttgtat tatttctccc taaagactca tttccagttg atggagttca
    11581 agatactcaa gaattttgat ttttatttca ctcattattt tctgtagaat tttgctcctc
    11641 taccggctta ttttctatag gaggtacata agtggaacct gcctcaaata tccttattgg
    11701 atcgtattga tatgtcatca attcgtttct acacaatcaa ttttgaataa tataataatg
    11761 tcatttattt agatcaggac aatttgtgta ggcataggct ggtctaccaa aattatcttt
    11821 tgttaattca aaatggacat gatatcctca caactctcct gaggcatttc aagtattacc
    11881 cactgttcat attttttgtc atctggtaac tttatctcaa gctttcacat taattgtatc
    11941 catatgagca taaacagcat acactatttc tcatttataa atatacttga ttctgactaa
    12001 atttccataa gctgaatttc gtcaagcaaa aaaaacttct ccatctcata tagaatataa
    12061 tggagtacca cgcgctgtag cgatatctac tcatccatga gctcaaattt tttggttcca
    12121 agaatcagaa taagaagcag ctcgtaaaat tgtataaata tctctataaa gttttacatc
    12181 ttgatatgct gaataatttg ctccatgaat tattggaaga ttgattttac acgaatttgg
    12241 catatcattt cgatatcatg ttctacattc tagtgtagaa acttgtttta agggatatcc
    12301 gaaggaagta ttatttggta ggtttgaagt tccagccatc atcaacccag taaatcacaa
    12361 aacacaaact aaaagtaatt ctcataaata aatgaattct tgatttattt gtcttattct
    12421 tgaatatatt ctatgtgatg ttttgtgctt gtatatatgt ttttccatta taatttatga
    12481 agattttaaa atattaacaa cactattata atggaaaaat caaatttgtc aaatttttga
    12541 taacttttta ttaaaatgac aaatgttttc aaaagtaaac tgggtttttt atgtataagt
    12601 atgtatataa ataaaaataa gttgaagttt tataaaatta aaatatacac attatgtttt
    12661 agaaaaaaat caaaatgaaa aaaattttat tcgttttaga ttattatcta ccacataaat
    12721 gaggtgtaga aaatgtattt gaaaatatat ttttgagatt agaaaaaaaa tgatataaaa
    12781 tagtcgtttt gactagccat tatgataaaa aattaaaaaa atatgaaaat gtaaattgag
    12841 tagaaattta tagaacatga aagtctagac tatcctttat gtgctctgct ataaaattat
    12901 gaaaacaaat tttaaggaat aataatattg atgtaattca cgcaagtaca tattgaggag
    12961 caattcctgc aagtatttta tgaaaaacat ttaataaaag gcttgttttg acagtgcatg
    13021 aagtgtttgg taatttgtgg tttaaatata aatgacttat tgctggagca atttatagat
    13081 tatttgaatg gataatattt agatttaaat ttgatgtttt tcattgtgtt tcccaaaaca
    13141 cagcaaatga agtaaaaaaa tattattgaa taaaagatgg taaaataaaa gtaatttata
    13201 atggactgga tacagaattt tggaatccaa gaaatgtttc gagtaaagaa atctttgaat
    13261 ggagaaaaaa acatcaacgg aatgataatt ttattttttt atatttttga catgctggga
    13321 aatcgaaagg aatagattat ttgatacaag cattgccaga aatattgaaa ttggaaaatg
    13381 taatgatggt ttttaacatt atcgactcca gaaggacaaa aaaaattttg gaagaattaa
    13441 aaaaacttga tgcaaacaat ttacagatat ttaatggatt tagtaaaaag gatttgagaa
    13501 aattggtagc ttccgctgat tgtgtaatag ctccatcaat ctctgaatga tttggtagtg
    13561 tccatagtga ggctgtagct atggggaaag tgcttattac tacaaaaact agtgcaattc
    13621 cggaggtagt ttatggtaaa gtaaaattta taaaaccggc agataaggaa tgaattattt
    13681 tggcttgtaa agatgtaatt gagtgaaaat ttgaaaacat tcctgaaaaa aaatttagtc
    13741 gagataattc tgtggaaaaa attgaaaagc tgtattaatc gtaagaataa aatttgtaaa
    13801 gttaaaattg ctgaaaactt atcaaattag tcaaataact tgatgaacct tataaacctt
    13861 taaacttttt actaagaagt atctaaatac tcatattttg cgaagaaata tcttttcacc
    13921 atattttgga tcaaatttca tcaaaaattt ttgtgtctca agttgtataa aaatctactt
    13981 ttcagttttt ttttaactta gactcaatct ctggatgttt tgcaagataa tcaataaatt
    14041 tgattgctga gttttgagaa ggatctatta tttttcatga aaaaagttgg gaaaaataat
    14101 ctttgtaaac ggagaaatga gtacatccta aaactaatgc ttgtaagttg tgggggaaca
    14161 ttgctaggta tctatctatt tctgtaagaa tttcttgttc ttttgctcaa agctctacca
    14221 tatctactaa ttttggagcc attacaaaat gaaaatctgg tttctgtttt catcaaaatc
    14281 tataatataa atcattgtat atgtctgatg tgattgtggc ttgagtagcc aatattcata
    14341 tagataatca aatattttcg gataacaata tttcttctac tcaaggaacg gtaattgata
    14401 atgttttttt gtctggatat aatgtctgtc gttttctaat agaatgagca gctgctgtat
    14461 tacaagctat aataactatt tctgctcaat tatcaaaaaa ccgattaagt gcattaaatg
    14521 taattttttg tatttcttct caagattttt ttcaaaaagg acaattttta ttatctgcca
    14581 aaaacatata atcatattgt ggatataact tatgaaagta tttcatagtt tgtaatcaac
    14641 caaatccact atcaaaaaat catattttcc ccattatttt tttaaaaaaa taaaaaaatc
    14701 agaaactaat atatagttaa ttgattcttt tgcaataaaa gtttttttta ttatagtata
    14761 tgtatgtcag ttcataaagt tgaaagttat aaagttgaaa gttaatgaat actttatatt
    14821 aagaacttta tgaactttgc aaacctgtct gccccgcagg actgcggggc aggctttatg
    14881 aactttataa actgtttaac ctcttacctt aaccatgttt gtagtttttg gaaaatgaaa
    14941 agttggaagt agtttaaaaa aattattgga caaattaagt ttggagaact ttatcatgga
    15001 tgatcaagat cgaaatgatg aaattttatc aaaatcagaa aaaataatcg tatctccttg
    15061 aataaaacaa aaccataaac tatatttgaa atataaaaac aaaatttttt ctgaattaaa
    15121 tttcttatga gatgtggtaa aaaatttggg attaagcgat agtttagaaa taatttgagt
    15181 aacatgaact aattgaaaat ccacatcagt acacataatt tacaacctat ttaaatgaat
    15241 ttttgaaaaa ttacatataa aaataaatat ttatttggct tgaaactttt gaactcccct
    15301 atccgaaacc ctatatgaaa ttatttcttg aaaaaatgaa aacaaaaatc taatcatttt
    15361 ggaaacatca tcttttatgt tgtacaattt aaataatttt aattttgatt acagtataat
    15421 cacaaattta tgagtagatc acttggattg gcataaggat ttgcaagaat attttgatag
    15481 taaatttaac attgtagact ttaccaaaaa ctatgctata atggataaag ataatattaa
    15541 tctttataaa aaaaatagga ctacaaaagc agagatagaa gaattccaac aagattttga
    15601 tttgaataaa accaactttt tatgaaaaca taacgaatga aattggaatg caagtttgaa
    15661 aataataaaa aaatattttg aaaaaaacaa tttgaaatgg aatgaagaaa ttttttggga
    15721 agtggctaaa aatattgaac cactagatca tagaattaaa atggtgaaag agttttgatg
    15781 aattaaaatt tacgatgact gaatatgtac atcttcacaa gcattaaatg ttgccctaaa
    15841 tagttttaac gaaaaaataa cattaatagc gggaggttat gataaatgag atgattacac
    15901 ttggctcgcg aatgaattgg aaaaaaaagt ttgatttgcg tgtttgatat gacaaactgc
    15961 taaaaaattt gaagtaattt tgaaagaaaa aaatattgac tatcaaatat ttgaaagctt
    16021 gagagaggct gttcactatg cgattagtaa agcaaaaaat ctttgaatta aaaatatttt
    16081 attttctcca tgatcggcaa gtttcgatat gtttgaaaat gtttatgata gatgtgatca
    16141 atttttaaaa ataattgata ttttgtaaaa atgctcttga aatttgtttt gtgttttgtg
    16201 tatattcaaa tgttaaactt aaaaaaaatg tgagtaattt ttaaaggaag aaagtaaata
    16261 aaattgtaaa atactgttta gcttttgtaa aagctaaaaa ggtcgggaca taagaaatat
    16321 attttaaaaa aattattaga aataagaata aaatttgttt gttattggat atgaaatttt
    16381 tatactgtga attaggagtt tgtaaagttc ttaaagtttg taaagtatta attaacttgc
    16441 aactttataa cttgcaactt tcaacttatc acttgtaact ttataaactc actattttta
    16501 acttttaacc gagatcaaag tgttaataga cattgtaaat tcatacgaca aagagaagaa
    16561 agaatttctt ttggggattt gggaggatgt gatattacat ctagcaaaac aaatagaccc
    16621 aaaaaagatt ttcacatttt tacaaaaagc ggggataatc agtattgatg aaaaagagca
    16681 aaaagtatat gtatgactag caaatgaatt tgctatgagt caggtgaaaa agtttttggg
    16741 gaaacaaata aaagagacaa taaaggaaat ttacaactct caatttgaca cggaattcgt
    16801 aatttatact ccgttttcaa acgataatga attgactgct gacttgaaaa aacttttaca
    16861 attaaaggaa aacaaaaagg aacacactat agaaccaaaa ataaaaaaca aattgtatga
    16921 atttttcggg atattatttg atccgattta taattttgaa aattttgtag tgtgagcaaa
    16981 taataatttt gctttttctg ctgcgaaagc agtttctgaa aatccttgaa cagcatacaa
    17041 tcctttattc ttatatggta atgtgggact aggaaaaact cacttaatgc aagcaatcgg
    17101 aaataaaatt atgcaagacg atccagaaaa aaatgtaata tatttaccaa caagtaagtt
    17161 gattgatgaa attgtggagt ctttgaaaaa aaataaattg aatacccttt tgtctaaatt
    17221 caatagtata gatgttttac ttttggacga tgttcaattc ttatctggga aagacaaaac
    17281 acaagaaata ttccacaata tattcaatga ttttcatatg aacaagaaac aagtaataat
    17341 tagtggagat cgccccccaa aagaactaat aaatattgaa ccaagactaa aaagtagatt
    17401 tggattggga cttgtagcag atataaaagc tcctgactac gaaactaggg ttgccatatt
    17461 acaatcaaaa ttagaacaaa aatgagaaga aatagattat aattttttgg aaaagatagc
    17521 aaaatatgta gtttgaaatg tgagagaatt agaatgagcc ttgaatattt tgattactag
    17581 aaaaaaactt atgtgaaact ctttgagcga acaagatgtc acaattgcat tggaaacttt
    17641 atgatatgga caacaagaaa ctatagatat gagcaatata agtgaatgaa ataaaaaaag
    17701 tgaaaagaat ttttccaaca ttgtagaaat ggtggctcaa tactacaatg tttcagtagc
    17761 agatctaaaa tctgacagta gaaaacaaga aatcacagta gcaagacaaa tgcttatgta
    17821 tttggctaaa acacattttg gatggacact agaaagaata ggtgattatt ttggtgggaa
    17881 aaaccatacg agcgtgattt atgctacaaa caatatagaa aaaaagatca aatttgataa
    17941 agatttagca catgactaca atgtttttgt agaatggttg gaaggataag ggagttggaa
    18001 agcctgcctg tagtaaagtt tgaaagtttg taaagttaaa atttactttt tatacaaata
    18061 ttatgtttct tttcaaaaaa aactctttaa cacttaatga gattaaagaa aaacccttta
    18121 agttagaaaa ggatttacaa gaaattgctg aaaataattt agaaaaagtt tttggattag
    18181 aatttgtttg tagtgaattt gctgtttgag agtttagaat agatacactt gcttatgatc
    18241 cagaaaataa gtcttttgta attatagaat acaaaagaga taggagtttt tcgatagtag
    18301 atcaatgata ttcttatctt gcactacttc tcaacaataa agcagaattt gttttaaaat
    18361 ataatgaaga atttaaatct cctattcaaa aaagtgatat aaattgggaa tcatcaaaag
    18421 ttatttttgt tgctaatggt tttacttctt atcaaaaaaa tgctatcaat tttaaagatt
    18481 tgcctataga attatgggaa agtaggcaat ttgaaaatga aataatttta ttcaatcaag
    18541 ttaaagctag taaaacaagc gaatctataa aaacaatcag taaatgagat aaaacctttt
    18601 ctaaaataac agaagaaata aaaacatata ctatagaaga tcattttaag gaatgatgga
    18661 atgaaagtaa ggaaatatac gaaaatttta aaaataaagt tttagaatta gactcaagaa
    18721 tagaagaaga tcctaagaaa tggtatattg cttataaggt aggcaacaaa gttcttattg
    18781 aattatatgt ttataagtca aaattaaaca tagattttat gaaaattgaa ccaaaagatt
    18841 taaaggatcc tgaaaaaaaa gtattttaca aaaaaaatag tataaaacat tttaaaagac
    18901 acatatctac tttttctatt aataatgaag acgacataga ttattgaata atgttaataa
    18961 aacaagtttt gaaaaaattc tttttataaa atataattaa atcaaaatgt cacaaaactt
    19021 agaggaaaga gtactaaaaa tagaacaaag aaatcaaaaa gtagagctag acaaagcatg
    19081 ggaaaccagc tggactagaa gatttttgat tgcaataatg acttacatta ccatagtttt
    19141 gttctttttg gtcgccaagt taccaaaacc ttttataaat tccatagtac caacagttgg
    19201 gtttgtatta tctactctat cactccccta ttttaagaaa ttgtggataa aaaaaagaaa
    19261 gaaataacta caatttatta aaggattaag aatagtttat atcaaaactt ttatactata
    19321 aaatttagaa aaaatgaaaa aaattacttt atttattttg attggattta gtgttgtttt
    19381 acttggatgt aagaatcaag ggattgttaa tgaagattgg aatttaatag acaataatga
    19441 ggaagaatta gtatcagatg aattagaaat tttggaagat tatgaaaata tagaagaaaa
    19501 taacgatagt acagaaatta ttgaagagaa agtagaggaa gtagtagaag aacaagtaga
    19561 aaaacaggtg gaagatgtaa aggtttctaa tactcaagct tcaaaaacca atacagtaaa
    19621 agaagataat ttagctaaat gtttgacttc taaatgaata aaattgtacg gtatggcagg
    19681 atgtccatat tgtaaagctc aaatagaact atttggagat gattttcaat atataaatta
    19741 tatcaattgt tctgaagatc ctaatgtgtg tgtagaaaaa tgaatttccg gagtacctgc
    19801 ttgggagttt attgatggaa gtataaaatc tggtctacat agcttagaag acctagctga
    19861 tttagtagga tgtaatttat aagttaaaac ctactttatc ataaatatct ttgatttttt
    19921 tctcagaaat ttcgtttaca tatttagcat tttttgctaa caaatctttt acttcttgat
    19981 cagaaatttt attgtatttt tcttgtattg gacgcaaaaa atccatcatt ttttcaaaca
    20041 aatattcttt tgcatattta taacttaatc atcatgctag gtatttcgcc ctaagttctt
    20101 tatcttctgt ttctgtgaga aatagtttac aaagattgta aacattacat tcatctggat
    20161 tttttgactc ctctactgtc tgtgagcctg tcggaatctg ttttattttt tttagaatag
    20221 tggcttcatc atctagcaat ccgatatagt tgttgtaaga ctttgacatt ttactgcgac
    20281 catctgttca aattataagt cacaattctg gattgatttt tgcttctggt aaaacaaaag
    20341 tttctccaaa tgtattatta aatttttgag caatatctct agcgtattct acatgttgtt
    20401 tttgatcttt tcacactggg accaaatctg catcatataa taaaatatct gctgccatca
    20461 atataggata acaaaaaact caaactcaaa tttcttttgc ttttcattta tttagagcat
    20521 ctttgtatga atgcattctt tccataaatc acatatgagt aatacaagtc aaaatccgat
    20581 ttaattgggc atgaccagga atatctgcag gattgtaaat tacaaattgt tttgtatcgg
    20641 ctccacaagc taaataaagc ttgattgcca tcattgaatt atttttcatt tcctcttgat
    20701 tatgaatttg tgtaaatcca tgcatgtttg caagaaataa aaaaaattct gaatctgggt
    20761 ttgccttggc taaatctatc atctgtttga tagagccaaa ataatttcaa agatgtaatt
    20821 gtgatcaagt cggctttaat caagtaagaa atctcatttg gagggtaatt gttgaaataa
    20881 aatttattga ctaataactg attttataag tcaaagaaat aatggatgtg gtttgttgat
    20941 cctagatttg aactctggat gagcttgtgt tgctacataa tatttgtgat ttgggaattc
    21001 aataaattct acaattagtg catcaggaga agttcaagat actacaagtc atttttgttg
    21061 taatatatca tgatactttg gattgactgc aaacctatgc ctatatcttt ctacaaccaa
    21121 attttttgat aaatcaattt gattaaactc tttgtataaa tttcggacct ttgtatctgg
    21181 ttttaaaact gcatcataag atcataagcg catagttcat catctatttt caatatcttt
    21241 ttgagacggc ataatatcta taacattatt ttttcaatct ggcttgaact cactagaatt
    21301 tgcatcaaca atattacaaa tatttcttgc aaatgctatt gtggctattt gaagtccaag
    21361 acataatcat aaaaatggaa catttttttc tctacaaaaa ttagctatat ttatcattca
    21421 ttctatccct ctatcgccaa atcaacacgg gatcacaact gccaaaatat tcttttgatt
    21481 tattagattt ctaaaactat cactccaaaa gtctgactca taattttctg aatctactcg
    21541 ttctattttt atttttgtat catagaaagc tccagcatgt tttaaagatt ctaacacact
    21601 aaggtaagaa tcatctaatt gagtatattt tccaacaagg gctatcgtaa tttctctttg
    21661 aggattgagt agtttattta cggtagcttc ccaagatgac atatctgctt tttttttctc
    21721 tccaaacatt ctttcaataa tatatttatc caatcattgc tcttgaaaag aaagtggtac
    21781 ttgataaata ttttgatcaa ttgtttctat gatacaatcc tcttctaaat cacaaaaaag
    21841 ggataatttt tttttgattc cattttctaa cggtactgga ctcctacaaa ttagaacatt
    21901 tgcttgaata ccgagctctc taagtttgat aatagaatgc tgaatagctt ttgtcttcat
    21961 ttctcatgaa gtggatattg taataatagg ggctacatgg acaaataaaa cattttcttt
    22021 tcataaatca tgcctaagtt gtctcatagc ttctataaaa tgtggtcatt ctatgtctca
    22081 aactgttcat catatttcaa taatagctac atcatttcat tctactaatt tataaactct
    22141 atcttttatt tcatcgataa catgtgggat cacctgtact gtttttcata aataatctca
    22201 tctcctttcc ttttgcatca cagagtaata tatctgtcag gtggtcatac tgctatctct
    22261 tgttaattct tcatttataa acctctcata gtgtcacaaa tctaagtctg tttcgaatca
    22321 gtcagtggtt acaaaaactt ctccgtgttc atatggagac atagtcccag catctatttg
    22381 taaatatgga tccatttttg ccatagtcac tttgtatcca gcagatttta aaattctacc
    22441 gatagaagct gctgtaattc attttcctaa tcctgaaata actcctcaag taacgaatat
    22501 tagtttcatt gtgagtaagt aaaatttata aactattaaa acgagagcgt gtaaggataa
    22561 taaataaatt tttaatttga ttacattttt ctttttactc ttttataatt tttttgcaag
    22621 tagtttaata aaaaacttta aaatttgtat tgaaatgaaa gctatcttaa atatacatta
    22681 tttagtgatt tttgatttat cttatttttt gttgtaaaat gttaaacaaa aaacggctaa
    22741 aattattaac attggggatt attctcttgt gatctgtact ttttgttttt gcagaaactg
    22801 gaaaacagga aaatattgaa gaagagaaat tggcacaaat tatgatagat actgggacca
    22861 atactattta cataagctga tatgtaagta cttgaaccac ttcttgagtt attgatactt
    22921 gaaaaataga agagcctcca gttttaataa ataatgaacc aaaggactta gaaacagaat
    22981 tttctgaagc tttggcttgg atgtatgcaa actgattaac aaagtatgat aataaagaag
    23041 actatagaat gtttgattta gtttctcgtg aagaagctgg taaaattata tgacaagcat
    23101 atgttgcttt gggatatcca gatatagaaa aaaattctag ttgtgatttc tgagatgcaa
    23161 gtaaatttaa tccaagctta gcaccacata ttgctaatgt ttgtaaacga tgattattta
    23221 aatgatccaa ttgaaattat atgcctctca ataatttaac aaaagcggag gctatggcgg
    23281 tacttttgag aatgtttgaa tgaaagatgt cttatgagtt acaaattcct tggcgagaac
    23341 aatattatac taaatgaaaa cttataggat taaccaatat tgaagatata aataagtttg
    23401 ataagggatt ggaaagatat gaaatagctt tgatggtaca tagactaaga agtatagtaa
    23461 caaatcctca gttgaaaact atggcactaa atttattatg acaagtactt actccaaata
    23521 ctgtaggaat aatggatggc tgaaccgtta tagcaaatct cgatactttg gtttgaggaa
    23581 tagatcctta caaagatcca gaacttttgg aagcaattta tcggatgtac gacaatgctc
    23641 taacaattca taaaaatcca aatgattaca ggccatttga tacattaaat aaagtagcag
    23701 cggcaaaaat ttttgataaa ttttcaacta tgcttagctt gtctggtagt gaggcttttt
    23761 tacctaatca atgtgaattt acagacatag gggatttaag taatgaagac cagcaatata
    23821 tagtaaatgt ttgtaaaaaa tgaattatga aatgaaataa taaaaagttt gtccctgata
    23881 gcaaattaaa taaatcacat tttgtggttt ctctagttag gatgtttcaa tgaaaatacc
    23941 tagatgaaaa tgtgagtcct tggtggcaaa actatttcaa tgaagcaaag gagcttggga
    24001 tagttgttcc tagtgatgct atcacttttg atactcctat tagtagatat gaggtggctt
    24061 tattcttgta caaattcaac ataaaatata agatgttgag taatttgaac aatgacagga
    24121 tagcaaatga aataattagt acagtagaag gatctattag tacgggaata aatggtaaat
    24181 taaaatcaaa tgtatatttg gactcaaatt tactaaaaag gtgatcattt gacctatgat
    24241 atatagaaac tttgggaact agatataaaa tagttaaatc ttctgaatcc acatattttg
    24301 ctgacaattt tgtgtggtat gggaatgtat ttgatttagc aagtgatgaa gagctgggaa
    24361 caattaattt tgtagtaagt aattgatgat atgtaataga agcaacgata agatttgatg
    24421 gatgagatac ctataaaata ctgccgattc aatgaactag tgcatattat ttattacaag
    24481 tattataatt taaataaaaa atattggaaa ttttagatac taacacggtc cttgtgaagg
    24541 taaaaacgat aaatatagat tctgtggtcc aagacaatca agaaaatctt agacctgata
    24601 gttttgatag ttttatatga caggaacata taaaaaaaat tttacaaact gctataaata
    24661 gtgcaaaaaa aagaaaagga agtatgggac atattttgtt ttgtggacct agtgggtttg
    24721 gaaaaactac tatggctcat atcattgcaa agcaaagtgg tgtaaatatg aaagtagtga
    24781 caggttatgc tatcaacaaa cctgctgaaa tagtaagtat tttaaacagt ttggaagaat
    24841 gagatattct atttatcgat gaaattcaca gactcaagcc aaatgtagaa gaagttttgt
    24901 atattgctat ggaagatttt gtgatagata tggtaatgcc agaatgagga agtgtaagaa
    24961 tacctttgaa taaatttact ttaatatgag ctaccaccaa atgagaatga ctttccaaac
    25021 cgatcaaaaa taggtttgtt tacgattttc attttatgga ctacaatgcg gaagaaaaaa
    25081 agataattat agatatatat ctaaaaaagt attgagtacc aacaaacaaa aatgtgattg
    25141 aaaaaattca caataaggtt gattctgttc ctagagaaat acacaattta tgtataaaaa
    25201 taagggattt ttttgtaacc gaatgaaaaa acgaaaaaga attcactcaa gatttacgaa
    25261 aaaggttttt gattcatagt aagatagatg atggctgaat gtctcctatt catcaaaaat
    25321 atctaaatat tttaaagaga aacgacagac ctatgggtat aaaatccatt gctatacaat
    25381 tgtgaatgaa tgaaaaagcc gtagaagagg acatagaacc cttattgata aaattatgaa
    25441 agatagagaa aacaattgga tgaagaatcc tcgtgagtta aaagttataa agttgcattc
    25501 tcctaccacg ggctgttcgc ggagctatcc aattgcaccc gggtggtagg ataagggggg
    25561 taggcaagaa ctttacaaac tctaagaact caattaagct ttagctttta attctttaaa
    25621 aaatgacttt agatgcatca ctaacatcca gtttagacac tcaaacacta ttag
//
LOCUS       DAOSID010000007        20951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_2793_length_20951_cov_14.687787, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000007 DAOSID010000000
VERSION     DAOSID010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 20951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20951
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2793_length_20951_cov_14.687787"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2051
                     /gene="topA"
                     /locus_tag="PK674_00980"
     CDS             <1..2051
                     /gene="topA"
                     /locus_tag="PK674_00980"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=25
                     /product="type I DNA topoisomerase"
                     /protein_id="HOG15138.1"
                     /translation="WIATDEDREGEAIGRHVANELGLDIKTTARIVFHEITKKAIEHA
                     IANPRTIDMNLVDAQQARRILDRLVGFELSPVLWKKISRGLSAGRVQSVAVRLIVERE
                     REIEKFKSANFFKTVGTFVGDSKQNFNATLNTDFKKIDDVKSFLELCKSSEFQISDVE
                     TKPGKKTPSAPFTTSTLQQEASRKLGFSVSRTMQIAQKLYEAGHITYMRTDSVNLSQD
                     AIFAAQTEITKNYGKEYSNPTNYKSSTKGAQEAHECIRPTHLENPIAGADAAQKKLYD
                     LIRKRTIASQMAPAKIEKTKATINISNHKNKFIAEGEVVKFDGFLKIYIESNDDENEE
                     NNSILPKLEKGEKLNMLEIISTEKFKKHPPRYTEASLVKELEKKGIGRPSTYAPTIST
                     IQKRGYVILDSRTGEKRDYFEVILKNNNISTNTKSQNTGVEKNKLFPTDIGRTVTDFL
                     MENFNNIMDYNFTASVEEQFDLIASGDMQREKMLHEFYGPFHEDVVSAEGTERFSGER
                     ILGEDPKSGKPVLVRIGRFGPLVQIGSAEDEDKKFANIPIGKTIETITLEEALDCFKL
                     PREVGKYEGESVMSAIGRFGPYVKYKDLFVSIPKSLETDPMSITLEQSIELIKAKQEA
                     NANKYIHEFTHDGKKVEVLNGPYGPYVKYNKKNYKIPKGGKDATDLTLEDCLEIISKK
                     "
     gene            2392..3423
                     /locus_tag="PK674_00985"
     CDS             2392..3423
                     /locus_tag="PK674_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15139.1"
                     /translation="MLKLRYEYFSESYPRFGVYKIQEGEKIQHHILHEKFSKIVDTAF
                     EEEQFQHIFLLDESRFSFFRFDIFLEIETNFSIQKLQEITKEKMEEIEFKQRIPGKFL
                     TTYIDSIFINGEEKKHIIGESGQVFFRLYIIYIDTKTLNTIDSSFGKVRENPNIKILP
                     QSFHTTLFLRNSLKRDNFLLLYITESSAKVIKITNSFYESVNVINLGLGALKQMYKDN
                     GVYQYWYKDYNEIQQNVLAENLVVETLEFYSMLFFSWLEGQNCIGNDIFVISPIVKNG
                     HFMEVFNRKYREYTNNYIVPFHYSDSLETFNKEREPDDMDTLIYLNREKIDILFCKKI
                     DKNRDDNDI"
     gene            3514..4113
                     /locus_tag="PK674_00990"
     CDS             3514..4113
                     /locus_tag="PK674_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG15140.1"
                     /translation="MKNKKGFTLIEMLIVIVIIGILATAIIPRLNSARSRANDVARKA
                     DLNQVANALITYQLDVGSFPVSAGTTIDTISEELTKGGLTAIPKDPNNNGRFSGSNAL
                     TNPSQPFNGYYVYQTVEKNGIPNGGFILMTQMESAGQANYLICGAEDIIGNPNSNSYS
                     NIELCSKITYSKNATSCDKSNLNNCISNNLLQLRYIYKH"
     gene            complement(4186..5217)
                     /gene="murB"
                     /locus_tag="PK674_00995"
     CDS             complement(4186..5217)
                     /gene="murB"
                     /locus_tag="PK674_00995"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF014872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOG15141.1"
                     /translation="MQIQNNVDLTSYNTFKVKSIAKHFVQIQNESEIIALLDEPIYLQ
                     NKKYFLGSGANTVFVDDFDGLIIKIDILGKEILSKNDEEILIKVGAGENRHNFVLRCA
                     ENNFAGVENLAYIPSSVGATVVQNVGAYGIEVKSVVQTVFGINLDTKKSQTLENKECN
                     FGYRDSVFKNELKNNFIVTHVVFRLKKLQPSYKFNCDYAGINEKISELGLDLSAMTIS
                     DFVEVITQIRKDKLPDRETTGTAGSFFKNPVITKEQWKILQSRFPEIKGFDSDNDIKL
                     SAGQLIDLCGFKGKSNGKVGTYKNHALILVNEGIATGQDIEYFAKEIQTSVQDRFNVL
                     LEPEVIFVK"
     gene            complement(5323..6711)
                     /locus_tag="PK674_01000"
     CDS             complement(5323..6711)
                     /locus_tag="PK674_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15142.1"
                     /translation="MTDQNPNPATINPNTNDESFDIFGGSDDIFENSTILEPINDALD
                     GEEDNKVETEDLFIDTQEPETKNETYEYQELAPEKVEQGPVIEQPTSEFSNTDNNFYD
                     LDQNWNKENIENKEIDPFKNMGNNEDEKEDISDFEDTTLESTQESDENVDLEQADIED
                     SPQIDVLDDIDLVDMSGGNETSFTDTQEGEMVNEDFEHAEVNPFESEEAQEEEVQEGN
                     VEEKGNVEEKGNVEEEGNVEEEEDVEEEEDIEEGDVQEEDVQEGDVEEEEDVEEEVDV
                     EEEEDIEEEVDVEEEEDIEEEEDVELQEARTKETRIEIDKDATDLQKKFYELVHEIKS
                     AHKLVDKDLSEGFDILGGNDDRKRTMYKVFAGENFASIDKTETFKTEEKEEVNTLAFV
                     LENKSLQILINDELLYDEVEDLVNDQPKKLQTLEKLNKFIFLITEEYKKVEKQKKEKE
                     QKNKIKGIFRNF"
     gene            6797..7363
                     /locus_tag="PK674_01005"
     CDS             6797..7363
                     /locus_tag="PK674_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="M48 family metallopeptidase"
                     /protein_id="HOG15143.1"
                     /translation="MSGYEIIYSNNKHGYARIKEGKLIISIPKFLKYNKKFKQTLIEN
                     GEKLLKKHKKNKYIKSKDEENVLLFGEFVEKKELKGNLDKNLKNILLDYVSPIVDEYS
                     KKLGNNFTSISIRKASSKRGSCSHDQKIMFSQNLVHLPTKYIKYVVIHEVCHLKHKNH
                     SKEFWKEVENFLPNYREIRKEMRKMIIE"
     gene            complement(7423..7698)
                     /locus_tag="PK674_01010"
     CDS             complement(7423..7698)
                     /locus_tag="PK674_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15144.1"
                     /translation="MGNFKEVARKSFAKKFMGDRMIGIIGLKVAQDFFDDPNLEGFIK
                     FSTLHIKTQNQEIKIKAFRHKKEILDKINDVLENNYGYTRKIKDIRF"
     gene            7821..8432
                     /locus_tag="PK674_01015"
     CDS             7821..8432
                     /locus_tag="PK674_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15145.1"
                     /translation="MKKVVLLVFVFIGFFGIGFTSPYYAPNCINGKVGENNLSCQACE
                     DEGQLYNPVENIGGVQFYAGCCPKGSILQDDPQNPNQKICISNTVGNMGINMDSDCLI
                     NGQCSLNIYKTLGIRKSNQDPTVSTFFQDIVLGATMFLGTVITIILVVSGILYISAAI
                     SGKSNSADMAKKGIFNSILGLLFVVGSYAIIRLIQFIATAGGG"
     gene            8464..9357
                     /locus_tag="PK674_01020"
     CDS             8464..9357
                     /locus_tag="PK674_01020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOG15146.1"
                     /translation="MQLFWGIIGIIVIVYVRIWIINNAFKNKNFKRHLSFDVFILSIF
                     LVTFLYFYKDLLIFFNVEHLYFLENNGWKNVASFVFYCFAFIILLTGSFGNFKKKSSF
                     KFISTALILFCGLGLGGTIMGINVLFMYHIISSYAEEILKFSVGQNIFLEKQEVNKHT
                     NMSKGNKKFVLVKTDLMFFAIMAGLGFSVIENMFYLIMSYFSGGRSILMGMGRSVFAT
                     LLHIVSTGLIAFFVIKKYDNKGKNLLKYFVGILAGFGLHGIYNLSLSYNIKIVTIAIL
                     IVCYFVLTYLLFNSDLIYQKK"
     gene            9515..10825
                     /locus_tag="PK674_01025"
     CDS             9515..10825
                     /locus_tag="PK674_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15147.1"
                     /translation="MKLKEYFKIQQEYKIDENNKFFIYEKIISQKNKKNLRNIKIFDF
                     LSVKSFAYGFVMIILLVGIYGIYFFNGDFSYEGFMVKNGSNLVTADYIAQIVEFNGDF
                     YVKHDNNYYKTNRISNGDNVILKRGSEIIFNTNSGTSAKIIGPARFTLHKELTSYKLI
                     ISEGDFIKMESLNPFTDSMEIILNDITISSEKNINLLVTKQNNEYKINNQGSKILVKK
                     DNKTQEVESKQLLAIKENDITLITDIQDFEQAIVKQNISQTFVMTDKNTKDSEQFTES
                     FINEISTGDNEPANLELAKDLGFLDKKEIPNQEQTKELYSILNTDSILFDLEGLYKNQ
                     LLGNQEQYDDYKGSLERKIQKLCKIFRLDYQDVSLDIGIIDIKEQLEVSYHIPSKHLL
                     NLEIMSNWIKYIQSVENQLDIDIEEVDQLWNNLKTNPPSNLVFK"
     gene            complement(10981..11604)
                     /locus_tag="PK674_01030"
     CDS             complement(10981..11604)
                     /locus_tag="PK674_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG15148.1"
                     /translation="MKKINTNNGMLKKILIFAVGIAIIILFCTLFCKKSVMICPSVKE
                     YLAVADFKGEGIQVKKGDEIVVDYIGRLKDGTVFDTSIEQVAKGCDKYNEARDYSQGL
                     GFIVGAGQMIAGFDRGVEGMKIGQTKTIEIAPSDAYGEKDESLFMTVKKSQLQSSDQY
                     KEGDVLYAPNGQAVKVHKVTKDEVILDANHELAGQTLIFDITIKEIK"
     gene            12188..16375
                     /locus_tag="PK674_01035"
     CDS             12188..16375
                     /locus_tag="PK674_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="PKD domain-containing protein"
                     /protein_id="HOG15149.1"
                     /translation="METKFIKKLLLSLSFIGMGFVFAAIPSDYVANYDIETDRSSISN
                     IFIQIQANNKIGAETPVSTFGTLHQSFVNIFPKFPQDYNFQVVYQQCIQLSNTLSKVY
                     NTNTFASFMDNCYKPLSQILTNINTNFTVKANAIARPTSGPAPLTVTFDARSSVDPSN
                     ETIPSDNFFRYYRDVNGQDKAIGIGPVINHTFGEAGNYIVHLTVRSSNKTAKGVFDGK
                     KTLSIDVKPKAAVISVFANGKKLKTNEKMKFGTQEGAKGMLLDGSATMAIGGRQVLSH
                     SREITSANGFRFSKPGDGLPSAIKVILPHEGEYKVLLKTTDNEGNNISEQYSLIISDP
                     IAVIKKYPETGTTSNSFKFDAGASYSIVSRLRSFTWEIFDGQGNKMQTFQGKSINYQF
                     KQPGSYIVKLTVEDDLGQTNIETIQVYVDSSTPIAQFNIRPSETRKFASKFIFDANPS
                     FDVDAENGFDSLNYSRSFSNPKSTNVISTKEGDKVIEVSFDSIGEHKIKLTVQDKFGK
                     LGEIEKTINIASTLRPEIFVVPQATTWGNPINLVAQSNENVLSYQWNFGDGEVISTQT
                     NKVSHIYKKVGQYKITLTVHDEDGNENTITDKVFIGSKNLPLVIYKVIGENQDILGQN
                     EECLEIINGNEIVNPAYKVERYQTFTIDPKESVNAKGDKTNLAFHFQAKNSEIYRKDT
                     FKTKFDEIGCQFVDLTVEDLSLGINQKNRVWFKVYNALPRLKNLVLFFPQYGNEMGIG
                     FNENQARDITNAGFDPLIVKVQALDAVDPDGFISYFKRYYYDKNDPTRPIETKITPGN
                     IPYAYFSLPKIPGEFMFGVTLYDSDDGKNSSEQIIGNGPIVFFPPDTKRPDIPLVTLK
                     ADKTTVDVGDEVVFDVISKIISDRSDFVQERTIYYDFNGDLERDLITKNDRVSYIYTE
                     PNDNGYIPRAAVTYRGYKGMGEGGSIVVKKGLKPRMLTETAGKFVLFRDISLGEIEKS
                     TICLNLLDCKKKEDGYLMNTKKTPVFSFEYPEYKKYVASIDIEDKYANQANARQIIDL
                     TGVRNSDGEVVNYTGNFKLLSIPQSSENEDGDLEILVGNSLDNSVAFYLLFDDQDGKK
                     ECYVDLDISDGEDKDFYCNHVFFQKFDPRYESIIGKIYYQEGEEMLSKNLKVSFIDFS
                     IKLDEKTQVIYDRITKLINNSTNEGFKALLINLQKGIIDPVETQANLVAVQNYLLDES
                     VSSLTDEERKEIQRIINELSDSTSISAMGGTEYDIAKKEILQILPSNLRIEIERLFVE
                     FENVEGDDEQQLSQNDKRKRVLNEMLSLISEKITKNVENQKPDEIIKEDMDSIIMPNI
                     CKIMNYYEIASEKCASDDVKIIDDTAVKVERGARSGLKILLIVLLSSVGLLVAVIAFF
                     AIKAKMNKQEYSEDENA"
     gene            16459..18618
                     /locus_tag="PK674_01040"
     CDS             16459..18618
                     /locus_tag="PK674_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15150.1"
                     /translation="MNTVNKVSNFFEKGSSNFGKKFLIPLSIVLAISVVAKTVDDSKT
                     RLSNVLLEQIDREGVAEEYLKNNLFIKDRPITNEEFGHSITKIDKDVYQIKYSHQFEG
                     IPDFSEASRKEVILNVRFSYKNNNIYIEFEEVKIGKHKLKDNGALIMNNTVYDYDISQ
                     GFNKSIEINSKVNQDKTLVAIRNKIKEYEFSFLGLMEIIEEFENTQLTKLNDLSLGKE
                     VMKEGEFYYREMFLRENGKYRPIAKVYFDKYGNWEQKKTKEEFENKKSTALGTTISFD
                     VQNHADTVIPATANKNWDDPSTSYVQEKDIAVSISDNSKEQLKKKVKDHRQQIINIID
                     KAKVGPNREFKGFNKINSERVWSDKKEWLFDTKLISLELIDDTYVFQRGDKTQAIYFG
                     IENKGGEKDNIFLKNTNNQKVENFFVTINNNYYQVSLKNDELIIYKIKKEGMNVSNNL
                     PVFAGNQKLANILNAKNINIYYNVDGKNLEYRTKDGSLVTYIPCEKTEQGEYKLGRQS
                     INIDIPDLYNYPEFKKIVNDIQKIQKELNVLDVDILNSFGTLLNRKGVKLDHQELVRI
                     VDQGGTTHYCTIKQTKDGMRVDLDAKLYKDVEKTLDKLKLRLEIVKKLNNTKIRWVNK
                     GKQEDFTKFVGGGWGIGTRFISQNNIIDFISQASNEVELELLRGEGQQTKIIYDISAK
                     SWKMKLRGKSVVYISGQDYKIKINENGEVFLDPVEKK"
     gene            complement(18697..19278)
                     /locus_tag="PK674_01045"
     CDS             complement(18697..19278)
                     /locus_tag="PK674_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOG15151.1"
                     /translation="MKKDLVFFGIQGSGKGTQADLFSKDFPNCKHLELGQIFRALSSN
                     KNLISEYIADRMFQGKMLDDSLAFDLFDMCGHLLSQTDYMLIDGFPRSLTQMYHFLSK
                     ANAEQRDFIGVHFYLSKEKAIERLLKRAQEQGRKDDTVESITRRLDLFTQETLPVIEY
                     FQKIDKLITIDADQPIDSIYKNLLEQLKKHGAI"
     gene            19689..20840
                     /locus_tag="PK674_01050"
     CDS             19689..20840
                     /locus_tag="PK674_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15152.1"
                     /translation="MKKLVLGFLILMGIATINLAFAKTAIGDIFETQSFVEKEIKLEE
                     LSIKDDIPFGIITNILEQECSPTIINESKMIVEHLGTLRGKNIPKWKIETEMRKRFNL
                     LKKVILSIAKKDKTTFCKQKYITYGILEITQEIFNREKYGSTTGKIEDGKKIKNPETQ
                     TKTEMNTQSSITGELEHSSAEQKKDYVQLQHNTTNLTGKEKMLAQITERSVQTVLETM
                     VAQRLLGQEDIKILDGAIHIQYAKTCEKTIGSFHAVRSKSTQQMRFKEIKLNINICSS
                     EGFYNNFDKYIKQILTHELGHYVYFFKDKQTEKFDKICRNGKEKLCQNNGFFSTYAQN
                     NKEEDYAESFAYWYLDTFNGKEKKFGSAPASEIQNQKERYFDELIQSLK"
BASE COUNT         7819 a         2818 c         3251 g         7063 t
ORIGIN      
        1 tatggattgc aactgatgaa gatcgcgaat gagaagccat tggacgacat gttgcaaacg
       61 aattgtgact agatatcaaa acaactgcta ggatagtttt tcacgaaatt accaaaaaag
      121 ctatagaaca tgctatagca aaccctagaa ccatagatat gaatcttgta gatgcacaac
      181 aagctagaag gattttggat cgtcttgttt gatttgagct ttctcctgta ctttggaaaa
      241 agatctctag atgactgtca gctggtagag tacagtctgt agctgtccgt ttgattgtgg
      301 aaagagaaag agaaatagag aaatttaaat cagctaattt tttcaagaca gtttgaactt
      361 ttgtatgaga ctcaaaacaa aattttaatg ccaccctaaa cactgatttc aaaaaaatag
      421 atgatgtaaa gtcttttttg gaattgtgta aaagttctga gttccagatt tctgatgtag
      481 aaactaaacc aggaaaaaaa actccttcgg ctccgtttac tacttctacg ctacaacaag
      541 aagctagtag aaaactatga ttttctgttt ctcgtacaat gcaaatagct caaaaattat
      601 atgaagcatg acatatcaca tatatgagaa cagattcggt aaacctatct caagatgcca
      661 tttttgcagc acaaacagaa atcacaaaga attattgaaa agaatattca aatccaacaa
      721 actataaatc aagcacaaaa tgagctcaag aagctcatga atgtattaga ccaacacatt
      781 tagaaaaccc tatagcatga gcagatgctg cacaaaaaaa attgtacgat ttaatacgaa
      841 aaagaactat agcctctcaa atggctccag ctaaaataga aaaaaccaaa gcaacaatca
      901 atatttcaaa ccataaaaac aaatttatag ccgagtgaga ggtagtgaaa tttgatggat
      961 ttttgaaaat ctatatagaa tcaaatgatg atgagaacga ggaaaataat tcaattttgc
     1021 caaaactaga aaaatgagaa aaattgaaca tgctagaaat tattagtaca gaaaagttca
     1081 aaaaacatcc accaagatac acagaagcta gtttagtaaa agagttagaa aaaaaatgaa
     1141 ttgggcgtcc atctacttat gcgccaacta tttctacaat ccaaaaaagg tgatatgtta
     1201 ttttggatag taggacatga gaaaaaaggg attatttcga agtgattttg aaaaacaata
     1261 acatttcaac taatacaaaa tctcaaaata cttgagtaga aaaaaataaa ttattcccta
     1321 ctgatattgg aagaactgtg actgattttt tgatggagaa tttcaacaat attatggatt
     1381 acaattttac tgcatcagtt gaggagcaat ttgatctgat agcatctgga gacatgcaac
     1441 gagaaaaaat gttacacgag ttttattgac cgtttcatga ggatgtggta agtgctgaat
     1501 gaacagaaag atttagctga gaaagaattt tatgagaaga tccaaaaagc tgaaaaccag
     1561 tgttggtgag aattggtaga ttttgacctc ttgttcagat atgatcagca gaagatgaag
     1621 acaaaaaatt tgcaaatatc cccataggaa aaactattga aactattact ttagaagaag
     1681 cactagactg tttcaaactt cctagagagg tttgaaaata tgaatgagag tcggtgatgt
     1741 cagctatttg aagatttggt ccttatgtga aatacaagga tttatttgtt tctattccaa
     1801 aaagtttgga aacagatcca atgtctatta ctctagagca atctatcgag ttaatcaaag
     1861 caaaacaaga agcgaatgca aataaatata ttcacgaatt tacgcatgac tgaaagaaag
     1921 tagaggtatt aaattgaccg tattgaccgt atgtcaagta taataagaaa aattataaaa
     1981 taccaaaatg atgaaaggat gctacagatt taaccttgga agattgttta gaaataatta
     2041 gcaagaaata aaatgcaacc attcctactt tgaacaggga ttatctcaac ccctcttact
     2101 ctatcttaaa tcaccccctt aataccccct cttcgattac ggagttctcc gtaacaagcg
     2161 aataggggga aagtgaacaa gaagtaggaa agcccctctt cttcgaagag gggtttgggg
     2221 tgattgaaaa aagaaagaag gatatgggtg attgaaaata aaaaaaattg gtctccaatg
     2281 ggaaaaatag aaattaaaat ttattttttc ttaacaccac ctggtgcttg atcgcgaagc
     2341 tagctaattg cgtcctgatg ataaaacatt cttttaactt tataaacttt aatgctaaaa
     2401 ctacgatatg aatacttttc tgaatcttat cctagatttg gagtatacaa aatacaagaa
     2461 tgagaaaaaa ttcagcatca tattttgcat gaaaagtttt ctaaaatagt tgatacagca
     2521 tttgaggaag agcaattcca acatatattt ttattggatg aaagtagatt ctcttttttt
     2581 agatttgata tttttttgga gatagaaacc aatttttcta tacagaaatt acaagaaatt
     2641 acaaaagaaa aaatggaaga aatagagttt aaacaaagaa taccgtgaaa atttcttaca
     2701 acttatatag atagtatttt tataaattga gaagaaaaaa aacatataat atgagaatca
     2761 ggacaagttt tttttagact atatattata tacattgata ctaaaacatt aaacacaatt
     2821 gattcatctt ttgggaaagt cagagaaaat cccaatatca aaattttgcc acaatctttt
     2881 catactacac tatttttgag aaattctttg aaaagagata attttttgct tttgtatatt
     2941 acggaatcta gtgcaaaagt gataaaaatt acaaatagct tttatgaatc tgtgaatgta
     3001 attaatttat gattatgagc attaaagcaa atgtataaag ataattgagt ttatcagtat
     3061 tggtataaag attacaatga aatacaacaa aatgtattag ccgaaaactt ggttgtggaa
     3121 acacttgaat tctattctat gttatttttt tcatggctgg aaggacaaaa ctgtatttga
     3181 aatgatattt tcgtaatatc tccaatagtt aaaaattgac actttatgga agtttttaac
     3241 agaaaatata gagaatatac aaataactat atcgtacctt ttcattattc tgacagttta
     3301 gaaactttca ataaggaacg agaaccagat gatatggata ctttaattta tttaaaccgt
     3361 gaaaaaatag atatattatt ctgtaaaaag atagacaaaa accgcgatga taatgatata
     3421 taatttgttg ataaattgat ttatttttac tattgcaata gttaaagaat tctatatatt
     3481 gtggttagta tttttatatt ttttgtattt ccaatgaaaa ataaaaaatg attcactttg
     3541 atagaaatgc taatagtgat tgtaattata ggaatcctag cgacagctat catacctagg
     3601 ctaaattctg caagaagtag agcaaatgat gttgctcgta aggcagatct aaatcaagtg
     3661 gctaatgcgt tgattactta tcagttggat gtatgaagct tccctgtgtc cgcgggaact
     3721 acaatagaca ctatatccga agaacttaca aaatgatgac taactgctat tccaaaagat
     3781 ccgaataaca acggtcgatt ttcatgatct aatgcgttaa caaatccaag ccaaccattt
     3841 aattgatatt atgtatacca aactgtagaa aagaactgaa tccctaatgg atgatttatc
     3901 cttatgactc agatggagtc cgcgggacaa gcaaattact taatttgttg agcagaggat
     3961 attatttgaa atccaaactc gaatagttat tccaatatag agctttgttc taaaataact
     4021 tatagtaaaa atgctacaag ttgtgacaaa agtaacctaa ataattgtat ttccaataat
     4081 cttttacaac ttaggtatat ctataaacat taaaatcata ttatagtaaa caaaaatccc
     4141 acaaattgtg ggattttttg tataatcatt ttttgaatct agctattatt ttacaaaaat
     4201 cacctctggc tccaaaagaa cattgaatct atcttggacg cttgtttgta tttcttttgc
     4261 aaaatattca atatcttgtc ctgtcgcgat tcattcgttt accaaaatga gagcatgatt
     4321 tttgtatgtt ccaactttcc cattactctt tcatttgaaa ccacatagat ctatcaactg
     4381 tcatgcagat aactttatat cgttatcaga atcaaatcat tttatttctg ggaaccttga
     4441 ctgtaaaatt ttccattgtt cttttgtaat tactggattt ttaaaaaaac tacctgctgt
     4501 tcaagtggtt tcccggtctg gtagtttatc ttttctaatt tgtgtgatta cttctacaaa
     4561 atcagaaatt gtcatggcag ataaatccaa tcataattca gatatctttt catttattcc
     4621 agcatagtca caattgaatt tgtagcttgg ttgaagtttt tttaatctaa aaactacatg
     4681 agtaacgata aaattatttt ttaattcatt tttgaagaca ctatcacgat atccaaaatt
     4741 acattcttta ttttctaagg tttgagattt ttttgtgtct aagtttattc caaatactgt
     4801 ctgaacgacg ctttttactt ctattcagta tgctcaaaca ttttgaacaa cagtagctca
     4861 aacactacta ggaatatatg ctaaattttc tactcatgca aaattgtttt ctgcacatcg
     4921 aagtacaaaa ttatgtcgat tttctcatgc tcagaccttg atcaaaatct cttcatcatt
     4981 ttttgataaa atttcttttc ataaaatatc tatttttata attaaaccat caaaatcatc
     5041 cacgaaaacg gtatttgcac ctgaacctaa aaaatatttt ttattttgta agtaaatcgg
     5101 ctcatctaat agggcaataa tttctgattc attttgtatc tgcacaaaat gttttgctat
     5161 agattttact ttgaaagtat tgtagctggt taaatcaaca ttattttgta tttgcatggt
     5221 atagtatttg agcatataaa aattaaattt tatactttat tgtataagaa aaaaactctc
     5281 ctattcaata ggaaagtttt gtatattagt taatattgga atctaaaaat ttctaaaaat
     5341 tcactttatt ttatttttct gttctttctc ttttttttgt ttttctacct ttttatattc
     5401 ttctgtgata agaaatataa atttatttaa cttctccaat gtttgtaatt ttttaggttg
     5461 gtcgtttact aaatcttcaa cttcatcgta taacaactca tcatttatta atatttgtaa
     5521 agatttattt tctagaacaa atgcaagtgt attaacttcc tctttttctt cggtcttaaa
     5581 tgtttctgtt ttatcgatac ttgcaaagtt ctctcatgca aaaactttat acattgttct
     5641 ctttctatca tcatttcatc acaaaatatc aaatcactct gataagtctt tatctactaa
     5701 cttatgggct gattttattt catgaactaa ttcataaaac tttttttgta aatccgtagc
     5761 atctttatct atttctatgc gagtttcttt tgtgcgagct tcttgtaatt caacatcctc
     5821 ttcttcttca atatcttctt cctcttcaac atctacttcc tcttcaatat cttcttcctc
     5881 ttcaacatct acttcctctt caacatcttc ttcctcttca acatctcctt cttgaacatc
     5941 ttcttcttga acatctcctt cttcaatatc ttcttcctct tcaacatctt cttcctcttc
     6001 aacatttcct tcctcttcaa catttccttt ctcttcaaca tttcctttct cttcaacatt
     6061 tccttcttga acttcttcct cttgagcttc ttcagattca aatgggttta cttctgcatg
     6121 ctcgaaatct tcattcacca tttctcattc ctgtgtgtcc gtaaacgaag tttcatttca
     6181 tcctgacata tctacaagat caatatcatc taaaacatca atttgtggtg aatcttcaat
     6241 atccgcttgc tctaaatcaa cattttcatc gctctcctgt gtcgactcca atgtagtatc
     6301 ctcaaagtct gaaatatctt ctttttcgtc ttcgttattt cccatatttt taaatggatc
     6361 tatttcttta ttctcaatat tttctttatt ccaattttga tctaaatcat aaaaattatt
     6421 atctgtgtta gaaaattcac tggttggttg ctcaattact ggtccttgtt ctaccttttc
     6481 aggggcaagt tcttgatact cataagtttc attttttgtt tctggctcct gagtatcaat
     6541 aaataagtct tcggtttcaa ccttattgtc ttcttctcaa tctaaagcat catttattgg
     6601 ttccaaaatc gtgctatttt caaaaatatc gtcactacca ccaaaaatat caaagctttc
     6661 atcatttgtg tttgggttga ttgtagctgg gtttggattt tgatctgtca tttttattgg
     6721 aataatttat aaaaatctgt agtaatatta tatttagaaa atttttaaat gcaaatttta
     6781 gcctataaaa ttttttatgt caggatacga aataatttat agcaataaca aacatggata
     6841 cgctaggatt aaagagggaa aattgataat aagtattcct aaatttttga aatataataa
     6901 aaaatttaaa cagactttga tagaaaatgg agaaaaatta ttgaaaaaac ataaaaaaaa
     6961 taagtatatt aaatctaaag atgaggaaaa tgttttactt tttggagaat ttgtagaaaa
     7021 aaaggaattg aaatgaaatt tagacaaaaa tctaaaaaat attttattgg actatgtttc
     7081 acccatagtt gatgaatata gtaaaaaact gtgaaataat tttactagca tttcaatcag
     7141 aaaagctagt agcaaacgat gatcctgttc ccatgatcaa aaaattatgt ttagccaaaa
     7201 tttagtacat ttaccaacaa aatacatcaa atatgtagtg atacatgaag tttgtcatct
     7261 aaagcacaaa aatcattcca aagaattttg gaaggaagta gaaaactttt taccaaacta
     7321 tagagaaata aggaaggaga tgagaaaaat gataatagag taaccccttg ccacccgggt
     7381 gcaattgtta gctctgcgaa cagcacccag gtggtaaggg gattaaaacc taatatcctt
     7441 tatttttctg gtataaccat aattattttc caaaacatca tttattttgt ccagaatttc
     7501 ttttttatgt ctaaatgctt ttatcttgat ttcttgattt tgagttttga tatgtaaagt
     7561 actaaattta ataaatcatt ctaaattggg atcatcaaaa aaatcttgag ctacttttag
     7621 cccaataatt catatcattc tatctcccat aaactttttg gcaaaagatt ttctggctac
     7681 ttctttgaaa ttacccatgt ttttgtttta tataaattga ctatagtgta aaaactataa
     7741 ataaaatttc aagcaaaata aatttgaaat gtaaaacaaa attgatataa gctaattgtt
     7801 ttatattgaa attttttttg atgaaaaaag tggttttgtt ggtttttgtt tttatatgat
     7861 tttttgggat atgatttact tctccctact atgcgcccaa ctgtataaat tgaaaagtgg
     7921 gggagaataa tcttagttgc caagcctgtg aagatgaagg acagctatac aatccagtag
     7981 agaatatttg atgagtacaa ttttatgctg gatgttgtcc aaaatgaagt atcttacaag
     8041 atgatccaca aaatccaaac caaaaaattt gtataagcaa tactgtatga aatatgtgaa
     8101 taaatatgga ttctgattgt ttaataaact gacaatgcag tctcaatatt tataaaactt
     8161 tatgaatcag aaaatctaat caagatccaa cagtatctac atttttccaa gatatagtcc
     8221 tttgagcaac tatgtttttg ggaacagtaa taactattat tttggtagtt agctgaatac
     8281 tttatatatc tgctgcaatc tcaggaaaaa gcaattcagc agatatggca aaaaaatgaa
     8341 tttttaattc tatattatga ctactttttg ttgtatgatc ttatgcgata attagactca
     8401 tacagtttat agctacagct ggtggatgat aaaaatttat agtattgtaa ataaaacaaa
     8461 ataatgcaat tattttggtg aataatatga attatagtaa ttgtttatgt acggatatgg
     8521 ataataaaca atgcctttaa aaacaaaaac ttcaaacgac atttgagttt tgatgttttt
     8581 atattatcaa tatttttggt tacttttctc tatttttaca aagatttgtt gatctttttt
     8641 aatgtagagc atttgtattt tttggaaaat aatggttgga aaaatgttgc ttcttttgtt
     8701 ttttattgct ttgctttcat tattttatta acatgatctt tttgaaattt taaaaaaaaa
     8761 tcttctttta aatttatttc aactgcatta attttgtttt gtgggctcgg gttatgatga
     8821 actataatgt gaattaatgt attatttatg tatcatatta tatctagcta tgcagaagag
     8881 attttgaaat tttctgtatg acaaaacata tttttggaaa aacaagaagt aaataaacat
     8941 acaaatatgt caaaatgaaa taaaaaattt gtcttggtaa aaacagattt gatgtttttt
     9001 gctattatgg ctggactgtg atttagcgtg atagaaaata tgttttattt aataatgtcg
     9061 tatttttctg gaggaagaag tatcttgatg ggtatgggta ggtccgtttt tgctacatta
     9121 ttacacatag tttcgactgg attgatagcg ttttttgtaa taaaaaaata tgataataaa
     9181 ggcaaaaatc ttttaaaata ttttgtgtga atactggctg ggtttgggtt gcacggaata
     9241 tataatttaa gtttatctta taatattaag attgtcacaa tagctatttt aatagtttgt
     9301 tattttgtgc taacttattt attgttcaat tctgatctaa tctatcaaaa gaaatagaaa
     9361 tagttacggg tttgtaaagt taaaagttat aaagttacag gtctacaaac tttacgaact
     9421 ttataaactt ttaactagtt tgtaactttt ttgtcacaaa atcatttttc attcgtatat
     9481 agatgtagag tggtatttaa attttttgtt tgaaatgaaa ttaaaagaat actttaaaat
     9541 acaacaagaa tataaaatag atgaaaacaa taaatttttt atttatgaaa aaattatttc
     9601 tcagaaaaac aaaaaaaact tacgaaacat caaaatattt gattttttga gcgtaaaatc
     9661 atttgcttat ggatttgtga tgataatctt attggtatga atttattgaa tttatttttt
     9721 caatggagat ttttcttatg aatgatttat ggtcaaaaat ggttcaaatt tagttactgc
     9781 tgactatatt gctcaaatcg tagaatttaa tggagatttt tatgtcaaac atgacaataa
     9841 ttattacaaa accaatagaa tttcaaacgg agataatgtt atcctaaaaa gatgatctga
     9901 aataattttt aatacaaatt ctggaactag tgccaagatt attggaccag ctaggtttac
     9961 tttacataaa gaattaacaa gttataagct cattatttct gaaggggatt ttataaagat
    10021 ggaatctctt aacccgttta ctgactccat ggagataatt ttaaatgata ttactatttc
    10081 tagtgaaaaa aatatcaatc tattagttac aaaacaaaat aatgaataca aaataaacaa
    10141 tcagtgaagt aaaatattgg ttaaaaaaga taataaaacc caagaagtgg aatcaaaaca
    10201 actactagct atcaaagaaa atgatattac cttgatcaca gatatccaag actttgaaca
    10261 agctatagta aaacaaaata ttagtcagac atttgttatg acagataaaa acacaaaaga
    10321 ttcagaacaa tttactgaaa gttttataaa tgaaatttct acaggagata acgaacctgc
    10381 caatttagaa ctagcaaagg atttaggatt tttggacaaa aaggaaatcc ctaatcaaga
    10441 acaaacaaaa gaactttatt ctatattaaa tactgattcc attttgtttg atttagaatg
    10501 attgtacaaa aaccaattac tatgaaatca agaacaatac gatgactata agtgatcttt
    10561 ggaacgcaaa atacaaaagt tatgtaaaat atttagacta gattatcaag atgtttcttt
    10621 ggatattggt ataatagaca tcaaagaaca actggaagtt tcataccata taccatcaaa
    10681 acatctatta aatttagaga ttatgtccaa ttggattaaa tatatacaat cagtagaaaa
    10741 tcaattggac atagatatag aagaagtaga tcaattatgg aacaatctca aaaccaatcc
    10801 gccaagtaat ttggtgttta aataaaattg gtttatttag tttaaaacaa attccacaat
    10861 cttattttta aactaaaaaa tcgtaggtta aaaaactcga caaacaacaa tgtcgagttt
    10921 tttatatata aataaagctc taatattaat aaagttttgg gggagattaa aaacaaaata
    10981 ttattttatt tctttaatag ttatatcaaa aattaaagtt tgtcatgcta actcgtgatt
    11041 tgcatccaaa ataacttcat cttttgttac tttgtgaact tttacagctt gtcaatttgg
    11101 agcatacaaa acatctcatt ctttgtattg atctgaggac tgaagttggg acttttttac
    11161 agtcataaat aaagactcgt ctttttctcc ataagcatct gaaggtgcta tttctatagt
    11221 tttggtttgt caaattttca ttcattctac gcccctatca aatcctgcta tcatctgacc
    11281 agctcatact ataaatcata atccctgaga gtaatctcta gcttcattat atttgtcaca
    11341 tcattttgca acttgctcaa tactggtatc aaacactgtc ccatctttta gtcttcaaat
    11401 ataatcaaca acaatttcat ctcatttttt tacttgaatt cactctcatt tgaaatcagc
    11461 tacagctaaa tactctttta cagaaggaca aatcattaca ctttttttac aaaataaagt
    11521 acaaaataat ataatgatag ctattcatac agcaaatata agtatttttt ttagcatacc
    11581 attatttgta ttaatctttt tcatttgaaa aaataaataa ataaaatcaa attccaggat
    11641 aagaaaaaac ataaaaattt caatattatt ttacgattta agaattaata attgataatg
    11701 aataattgag aatttattat tgtaagttta tcgagtttgt aaagtttata aagcctgccc
    11761 cgccccgcag tactgcgggg cggagtggcg ggtttgtaaa gttctagtat gaaattcctc
    11821 ccttggggga ggttgttttc ccccgcagta ctgcggggat aggtgggggt tttaccccca
    11881 actacactag agcaacttcc ccgcagggga agaatgtcta gtctgtgacc aaatccccct
    11941 taatccccct tgctaagggg gataatcatc aactttacaa acctgcctgc cggcaggcag
    12001 gcttgcaact tttaactttc aacttgtaac ttttaacttg caactttata atacatacaa
    12061 catattagga gagtatttga aataaagtca aattgtaatg taaaaactac atcaaaaatt
    12121 tgcaaaaaaa taactttatc aatataattg ggatacagta gaacttttaa aatatttttt
    12181 aatataaatg gaaacaaaat ttatcaaaaa actattatta tccctatctt ttattggtat
    12241 gtgatttgtg tttgctgcta taccaagcga ctatgtcgct aactatgaca tagaaactga
    12301 tcggagcagt atttccaata tttttataca aattcaagca aacaataaaa tttgagcaga
    12361 aactcctgtt tctacttttt gaacattaca tcaaagtttt gttaatattt tcccaaaatt
    12421 tccacaagat tataactttc aagtagtata ccaacagtgt atacaactaa gtaatacatt
    12481 atcaaaggtg tataatacta atacttttgc ttcttttatg gataattgtt ataagccatt
    12541 atctcaaata ttaacaaaca taaacacaaa ttttacagta aaagctaatg ctattgcaag
    12601 accaactagc tgaccagctc ctctaactgt gacatttgat gctagatcaa gtgtagatcc
    12661 gtcaaatgaa acaataccat ctgacaattt ttttcggtat tatagagatg taaacgggca
    12721 agataaggca atatgaattt gaccagtaat aaatcatact tttggagaag catgaaatta
    12781 tatcgttcat ttaacggtta gaagttcaaa caaaactgct aaatgagttt ttgattgaaa
    12841 aaagacattg tccatagatg taaaaccaaa ggcggcagtg attagtgtct ttgcaaacgg
    12901 aaaaaaatta aaaacaaacg aaaagatgaa attttgaacc caagaatgag ctaaagggat
    12961 gcttttggat ggatctgcta ctatggcaat atgaggaaga caagttcttt cgcattcacg
    13021 ggaaataaca tctgcaaatt gatttaggtt tagtaaacca tgagatggat taccaagcgc
    13081 aattaaggtt atcttacctc atgaatgaga gtacaaggtt ttgttgaaaa ctacagataa
    13141 tgaatgaaac aatatatcgg aacaatattc tctgattatt tcagatccta tcgctgtaat
    13201 aaaaaaatat cctgaaacat gaacaacaag caattctttt aaatttgatg ctggcgcatc
    13261 ctattctata gtttcaagat tgagatcctt tacttgggaa atttttgatt gacagtgaaa
    13321 taagatgcaa acttttcaat gaaaaagtat taattatcaa tttaaacaac cttgatctta
    13381 tatagtaaaa ttgactgtag aagatgattt atgacaaact aatattgaaa caattcaagt
    13441 ttatgttgat tcttctactc ctatcgctca atttaatatc aggccatcag aaactcgaaa
    13501 atttgcttca aaatttattt ttgatgctaa tccctctttt gatgtggatg cagaaaattg
    13561 atttgatagt ttaaattatt cacgaagttt ttctaatcca aaaagtacta atgtaattag
    13621 tacaaaagaa tgagataagg tgattgaggt tagctttgat agtatatgag aacataaaat
    13681 taaattaaca gtacaggata aatttgggaa acttggtgaa atagaaaaaa ctatcaatat
    13741 agcttctaca ttgagaccag aaatatttgt ggttccacaa gcaactactt ggtgaaatcc
    13801 gattaatctt gtagcacaaa gtaatgagaa tgttttaagc tatcaatgga actttgggga
    13861 ctgagaagta atatctacac agacaaacaa agtatcccat atatataaaa aagtgggaca
    13921 atataaaatt accttgactg tgcatgatga agatggtaat gaaaacacca ttacggacaa
    13981 ggtatttata tgaagcaaaa atctacctct ggtaatatat aaggtaattt gagaaaatca
    14041 ggatatactt ggacaaaatg aagaatgcct tgaaattata aatgggaatg agattgtgaa
    14101 tccagcatat aaggtggaaa gatatcagac atttacaata gatccaaaag agtcagtaaa
    14161 cgcaaaatga gataaaacta atttagcttt ccatttccaa gctaaaaatt ctgaaattta
    14221 tagaaaggat acttttaaaa ctaaatttga tgagattggt tgtcaatttg tagatttaac
    14281 tgtggaagat ctatctttat gaataaatca aaaaaatagg gtttggttta aagtatacaa
    14341 cgctctacca agattaaaaa atctggtttt atttttccct cagtatggaa atgaaatgtg
    14401 aatatgattt aatgaaaatc aagcaagaga tatcactaat gcgggatttg atcccttgat
    14461 agtaaaggtt caggcattgg atgctgtaga tcctgattga tttatatctt atttcaaacg
    14521 atattattat gataaaaatg atcctactag accgatagaa acaaaaataa caccaggaaa
    14581 tataccttat gcttattttt ctttacctaa aatacctgga gaatttatgt ttggagtaac
    14641 tttatatgat agtgatgatt gaaaaaattc tagtgaacaa ataatatgaa actgacctat
    14701 tgtgtttttc cctccagata caaaaagacc agatatcccg ttggttactt tgaaagcaga
    14761 taaaacaaca gtagatgtgt gagatgaggt tgtgtttgat gtgatatcaa aaataatttc
    14821 ggatagaagc gattttgttc aggaaaggac aatatattat gattttaatt gagatttgga
    14881 acgagattta attacaaaaa atgatcgtgt aagctatata tatacagagc ctaatgataa
    14941 ttgatatatc cctagagcgg ctgtaactta cagatgatac aaatgaatgt gagaatgagg
    15001 aagtattgtt gtaaaaaaat gactaaaacc taggatgtta acagagactg ctgggaagtt
    15061 tgtattattt agagatatta gtttgtgaga aatagaaaaa agcaccattt gtttgaacct
    15121 tttggactgt aagaaaaaag aagacggata tttgatgaat actaaaaaaa ctcctgtttt
    15181 ttcttttgaa tatccagaat ataaaaaata tgtagcatca atagatattg aagataaata
    15241 tgctaatcaa gcaaatgcta gacaaattat agatttaact tgagttagaa attccgatgg
    15301 agaagtggta aattatactt gaaactttaa acttttgtct atacctcaaa gttcagaaaa
    15361 tgaagatggg gacttggaga tattagtagg aaatagtttg gataatagtg ttgcttttta
    15421 tttgcttttt gatgatcaag atggtaaaaa ggagtgttat gttgatttgg atataagcga
    15481 ttgagaagat aaagattttt attgtaatca tgtgtttttt caaaaatttg atccaagata
    15541 tgaaagcata atttgaaaaa tatattacca agaaggagaa gaaatgttgt ccaagaattt
    15601 gaaagtatct tttatagatt tttctataaa attagatgaa aaaactcaag taatatatga
    15661 tagaattaca aaattgataa ataatagtac gaatgaatga tttaaggcgt tactaataaa
    15721 tcttcaaaag tgaataatag atcctgttga aacacaagct aatttggtcg cagtacaaaa
    15781 ttatttattg gatgagagtg taagctcatt gactgatgaa gaaaggaaag agattcaacg
    15841 aataataaat gaattgtcag attctacgag catatcagca atgtgaggga cagagtatga
    15901 tatagctaaa aaagagatat tgcaaatatt gcctagtaat ttgagaattg agatagaaag
    15961 attatttgtg gagtttgaaa atgtggagtg agatgatgaa caacagcttt ctcaaaatga
    16021 taaaagaaaa agagtactga atgagatgct gtctttgata tctgaaaaaa taacaaaaaa
    16081 tgttgaaaat caaaagccag atgaaattat taaagaagat atggattcta taatcatgcc
    16141 aaatatttgt aaaataatga attattatga aatagccagt gaaaagtgtg cttctgatga
    16201 tgttaaaatt attgatgata ctgcggtaaa ggttgaaaga tgagctagat ctggactaaa
    16261 aattttattg atagtgttgt tgtcttcagt ttgattgctt gtggccgtaa ttgcgttctt
    16321 tgctataaaa gcgaaaatga ataaacaaga atatagtgaa gatgaaaatg cttaaaaata
    16381 tataaaagca tttgactttt gtatgaaaat atataagctc aaaagagcta tatatttata
    16441 ttgtacaata ctgcaagaat gaatacggtc aataaagtat caaatttttt tgagaaaggt
    16501 tcttctaatt ttggaaagaa gtttttgatt cctttatcta ttgttttggc gataagtgtt
    16561 gtcgcaaaaa cagttgatga tagtaaaact agattaagta atgttttatt ggaacaaatt
    16621 gatagggaat gagtggctga agaatatttg aaaaataatc tgtttatcaa agataggcct
    16681 attacaaatg aggagtttgg tcatagtatt acaaaaatag ataaagatgt atatcaaata
    16741 aaatattctc atcaatttga gtgaatccct gatttttctg aagcgtctag gaaagaagtt
    16801 atactcaatg taagattttc ttataaaaat aataatatat atattgagtt tgaagaagta
    16861 aaaatttgaa aacataaatt gaaagacaat ggagccttga taatgaacaa tacggtctat
    16921 gattatgata ttagccaggg gtttaataag tctattgaaa ttaattctaa agtaaatcaa
    16981 gataaaactt tggtagcaat aagaaacaaa attaaagagt atgagttttc ttttttgtga
    17041 ctaatggaaa ttatagagga atttgaaaat actcaattaa cgaagttgaa tgatctttca
    17101 ttatgaaaag aggtgatgaa agaatgagaa ttttattaca gggaaatgtt tttaagagaa
    17161 aatggaaaat atcgtcccat agccaaagta tatttcgaca aatatgggaa ttgggagcaa
    17221 aaaaagacta aagaagaatt tgaaaacaag aaaagtactg cactatgaac tacaataagt
    17281 tttgatgttc aaaatcatgc ggataccgta attccagcta ctgctaataa aaattgggat
    17341 gaccctagca caagttatgt tcaagaaaag gatatagcag tttctatttc agataattcc
    17401 aaagaacagt tgaaaaaaaa ggtaaaagat cataggcaac agataataaa tataatagat
    17461 aaggcgaagg tcggccccaa tagagaattc aaatgattta ataaaataaa ttcagaaaga
    17521 gtttggtctg ataaaaaaga gtggttattt gatacaaaac ttatatcttt ggaactgata
    17581 gatgatactt atgtatttca gcgatgagat aaaactcaag ctatttattt tgggatagaa
    17641 aataaaggtt gagaaaaaga taatatattt ttgaaaaata ccaacaatca aaaggtggaa
    17701 aatttttttg tgaccataaa taataattat tatcaagtaa gtttgaaaaa tgatgaattg
    17761 ataatatata aaattaaaaa ggagggaatg aatgtaagca ataatttgcc ggtgtttgct
    17821 tgaaatcaaa aattggcgaa tatattgaat gcaaaaaata taaatatata ctataatgtt
    17881 gatggtaaga atttggagta tcggactaag gattgaagtt tggtaacata tattccttgt
    17941 gaaaaaacag aacaatgaga atataagttg ggaaggcagt ctataaatat agatattccg
    18001 gatctataca attatcctga gtttaaaaaa atcgtcaatg atatacaaaa aattcaaaaa
    18061 gaacttaatg tgttggatgt agatatatta aatagttttg ggaccttatt aaatagaaaa
    18121 tgagtaaaat tagatcatca agaattagtt agaattgtag accaaggatg aacaactcat
    18181 tattgtacaa tcaaacaaac caaagattga atgagggtag acttggatgc gaaattatac
    18241 aaagatgtag aaaaaacttt ggataaatta aaattaaggt tggaaatagt aaaaaaattg
    18301 aataatacaa aaatacgatg ggttaacaaa tgaaaacaag aagatttcac aaaatttgta
    18361 tgatgaggat ggtgaatatg aactagattt atatctcaaa acaatattat agactttatt
    18421 tcccaagcta gcaacgaagt agaattggag ttgttgcgat gagagggaca acaaacaaaa
    18481 attatctatg atatatccgc taaatcatgg aagatgaaat taagatgaaa atcggtagta
    18541 tatatttcat gacaggatta taaaattaaa ataaatgaaa actgagaggt gtttttggat
    18601 cctgtagaaa aaaagtaaga tagtttgatt ggaagagaat atacaaaaca acacaataat
    18661 attgtgtttt tttgtttaag ttttgctagt aaataattat attgctccgt gtttttttag
    18721 ttgttccaaa agatttttgt atatagaatc aataggttgg tcagcatcaa tggtaattag
    18781 tttgtctatt ttttggaaat actctattac tggcaatgtt tcttgtgtga aaaggtctaa
    18841 tctcctagtg atactttcta ccgtatcgtc tttgcgtcat tgttcttgtg ccctttttaa
    18901 tagtctttca atagcctttt ctttggataa ataaaaatgt actcagatga aatctctctg
    18961 ttctgcgttt gctttactca aaaaatgata catttgtgtt aaagatctag gaaatcagtc
    19021 tattaacata taatctgttt gggacaataa atgaccgcac atatcaaaca aatcaaaagc
    19081 taagctatca tccaacattt ttcattgaaa catacgatcc gctatgtact ctgatataag
    19141 gtttttgttg gaacttaatg ctctaaatat ttgtcatagc tctaaatgtt tgcaattggg
    19201 aaaatctttt gaaaatagat cggcctgtgt tcacttacct gatcactgaa tcccaaagaa
    19261 aactaaatct tttttcattt tttattttgt tgaatataaa attattattt ggtagtataa
    19321 ttattattgt tttaaaaaca aattttattt tgtttattcc aaaactatat ttatcttgtt
    19381 ataaataaag ataattactt tttttataag gagccttgag tgctactaga ttccatacat
    19441 tctcaatttc aagtattcaa cgaaaatgat ttggcataat caaataatga tataatatta
    19501 tatttttcat tttgtgagga ttttgtatta tttaggaaac aatatttaca aaaaaacctg
    19561 ttgattgcac aaaattttgg cttgttgtct agtttgtttg gacaatagta attttgttgc
    19621 aaaaaatttg acaaatttga ttttatagtt ataatattag tggttttaat cataaaaatt
    19681 ttaaaaacat gaaaaaatta gtactctgat tccttattct aatggggata gcaaccatta
    19741 atttggcttt tgcaaaaaca gcaataggag atatttttga aactcaaagc tttgtagaaa
    19801 aagagatcaa gcttgaagag cttagtatca aagacgatat tcctttttga attatcacta
    19861 atatccttga acaagagtgt tctccgacaa taatcaatga atcaaagatg atagtagaac
    19921 atttgggcac gctccgatga aaaaatattc caaaatggaa aattgaaact gagatgagga
    19981 aaaggttcaa tcttctcaaa aaggttattc tttctattgc aaaaaaagat aaaactactt
    20041 tttgtaaaca aaaatatatc acctatggta tactagaaat tactcaagaa atttttaaca
    20101 gagaaaaata cggttctaca actggaaaga tagaagactg aaaaaaaata aaaaatccag
    20161 aaactcagac aaaaacggaa atgaatactc agtcttcaat tacttgagag ctagaacata
    20221 gtagcgctga gcagaaaaaa gattatgtac aactccaaca taacactaca aatcttacag
    20281 ggaaagaaaa aatgcttgca caaatcacag aaagaagtgt tcaaactgtc ctggaaacta
    20341 tggtcgccca aagacttttg ggacaggagg atattaaaat acttgatgga gcaattcata
    20401 tccaatatgc taaaacttgt gaaaaaacca taggttcttt tcatgcagtg agaagtaaat
    20461 ctactcaaca aatgagattc aaagaaatca aacttaatat caatatttgt tcgagtgaag
    20521 gtttttataa taactttgat aaatatataa agcagattct tacccacgaa ctttgacatt
    20581 atgtctattt tttcaaagat aagcaaacag aaaagtttga taagatctgc cgaaatggaa
    20641 aagaaaaact ctgtcaaaac aatggattct ttagtactta tgcacaaaat aataaagagg
    20701 aagattatgc agaaagtttt gcttattggt atcttgatac tttcaatgga aaagaaaaaa
    20761 aattctgatc tgctccagca agtgaaattc aaaaccaaaa agagagatat ttcgatgaac
    20821 tgatacagag tttgaaataa aaatttttac taatatgttg aatatctctc cccaaacccc
    20881 cttgcttatc ttaaatcacc cctatcccct cttatatttt taatcatacc caagctcttc
    20941 cttttctaaa t
//
LOCUS       DAOSID010000008        20242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_2895_length_20242_cov_16.762124, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000008 DAOSID010000000
VERSION     DAOSID010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 20242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20242
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2895_length_20242_cov_16.762124"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..777)
                     /locus_tag="PK674_01055"
     CDS             complement(172..777)
                     /locus_tag="PK674_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15153.1"
                     /translation="MKKSEKTLKSTVKNCDNSIKRNFAKVNKYLGFSGWIIVIFVVFI
                     ISLVIYYQNRIANIESRGIIYYQGPYRIDRTLNVFDEFDRYFDRQDKYFKNLFREQEE
                     FIRRYGKNFDLDKARMVDMSDKSQQKYQKYYLQNGKVYSYEINYNNGVVNGNIVVDDI
                     EQRDVLIQRLQKLGLELSNSDLNIIFSGEFDNINSLLSILD"
     gene            complement(836..1321)
                     /locus_tag="PK674_01060"
     CDS             complement(836..1321)
                     /locus_tag="PK674_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15154.1"
                     /translation="MFKGKVYVYKNINGKEEKIEREFDNSQDFQSFVQNNEVSGLESF
                     SDTPRSPILGLGDWANLQNYFDNLIDRRLGLIYDGYDEEQDNYAGGNLIDLNKYESEL
                     KKIDYQKQHKDETLKHLKSTLQKLKDYKKKFKNEGREDMIESIDLDIKKIENEISKFD
                     K"
     gene            1642..1854
                     /locus_tag="PK674_01065"
     CDS             1642..1854
                     /locus_tag="PK674_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOG15155.1"
                     /translation="MKKQSFIKIEGSASVGTKGQIVIPKNVRDKFKLKFGNDLIVLSS
                     DNAILLVKSESLENMIGSFENIINDL"
     gene            complement(1859..2914)
                     /locus_tag="PK674_01070"
     CDS             complement(1859..2914)
                     /locus_tag="PK674_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOG15156.1"
                     /translation="MDFDPKKNYYEILGVSEDATEDEIKKAFRKAAVKYHPDKAGGNK
                     EKFQEVNEAHQILGDKQKRQQYDMFRKGGFGGFGGGNFGGFQTGGASFDFGDLGDIVG
                     NIFGGGFGSFGGGGGPRSGEDLKKKITITFDESYLGVTKKISYTRKRVVEGAEAKTCD
                     MCSGRGRVTKQVQSPFGVMQSQSTCPTCGGSGQQYFKDGKKLENGGLEEVKEILEVKI
                     PAGINHGAYLKYSNKGDDGQNGMPTGDLYIQINVSENPNYIRKGDHIYVNQEVTIYDL
                     VLGGEYEILHPSGKIRIKIPKGTQLDEQIKISGKGFGSGGIFAKKGDLFVVPKLKIPK
                     RLSKEQEVLWKKLQESK"
     gene            complement(3083..5971)
                     /locus_tag="PK674_01075"
     CDS             complement(3083..5971)
                     /locus_tag="PK674_01075"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG15157.1"
                     /translation="MFPKKYINKNKNIQRSSFPSSKFIYSRALSADQKVHPGALFLIF
                     MKDYISKMSLWGSDGLDFDINFALSSNWYSKLNRNNVNLKQLQLDLKKQVKQNIKFLE
                     KLGFSFFINENHFVVDEKFYIFLREVFVNLYKDGKIHLEKKVVSRSKELQTNVLKTNL
                     FIKKKKKKEFVIKYFIESKGVSISVPTKNLKTIFADVAVAVNPQDKRYKKLIGQNVII
                     PIINKNIPIIGDDSVVSFEGTGVVRITPGHDEYGLEIAQKHNLPTNIYAIDVDGNFTK
                     HVGDFVGKPVDVFIENVETYINDIGNLEQINETEEELMYCAKSGEELYKITMDQWGIK
                     YDYSLDFLLQNFETKDITIKGFDVENHILDYLKDLNFVNISNKSAKGTLIPVLYSEKG
                     QKYLINDDILVEQYKIAKSRKDLTLTAIILNLVLDNQLNDVFSIEELVDVLFSFDFSG
                     GVSKLSKYIDIYENTERSSLKNGLKGIKRLLGKIERDSEKIMILLELLGDSFAIKSDG
                     ERFSLDFSAIFGSQEPLVLQKQDSFNKDFLDTVWLLFKNNLGKSMEGYSGIKNLGNIF
                     IGTKDDKEFFVNLLLLGLDYSKTLFFSDSLFIPYLTDQESNIITNYNSKFLTKELSGI
                     LDLFNSDLLRLLLLLGQKENNKVILDTNNIEYIHFMLNKVWNAYRYIYNNYINGISKI
                     NIKELFNLIEQDISDYDNWILHGLKSLLEELKNKNKNILDFGSKILKFIINDLCENYL
                     ESTKIYEYESTPHVALFSFIVSLEILRPLMPLFMQEIEKLFFNKLKISNFDISTIGDF
                     NLKEKNYRMNIFMDIVDKLKKIKQNLGVKKHETVDVFVQANLEFINFLSEREDLLEAL
                     LNIRNIVYVKDYEKTPSDYEMDNVIDINIGAKSVSEQIGVTKNVLMEMEEDLKNKQEY
                     LQHMKSLISSILSTASMEIIEKKKQDIQNLQKEVEELEFKINKMKIKG"
     gene            complement(6035..7666)
                     /locus_tag="PK674_01080"
     CDS             complement(6035..7666)
                     /locus_tag="PK674_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOG15158.1"
                     /translation="MNKFYKKLAVGVFMFFSALVPLVSLAEEPDSFSVKVEPDTFGVG
                     QPVDITITALKNGEILKDYIGDVLFEINGLLPDDYVVPSMGFYSFLPQDQGTKTFSKG
                     LEIKKAGTYQIKVSDVTDLNYEFHGTATVVVGNASSDSSLKQINITSPIDSSIEKKST
                     INIIGSSFDLRNSPIDVYLNDMLVTSGQTDSMGNFNVYVGNLNSGPNTIQIKAVNINN
                     VLLGESNVVTVTYQPPFDGVFNSIEIIPSGVLKEGDKVTFTVSAVDEVSSAQMIFDDG
                     TSYPLDLLSPGIFKKDIIMTDILGNKNISVVLMYAGIPRNYDNVASIFVKENKGVGNV
                     RFVSTGVDGTSVIVSWDSRGHFPKYRVFYGTDQNNLQKTVDINATGVLIENLQTDTTY
                     YFQISPLDELSNISGDPSSIVEYNPQKVKASCVVQGIVVKSEKIGDKYFLVWDEVKNA
                     TKYEIYRSDWEDMSDKKLVGETTEASFEYFFDKYAEKDQYAYYQVQAICSDGTSVLID
                     QAQKVQVGPFEDTLLIIIVSLFLYAVYKLYRTQKY"
     gene            complement(7685..8122)
                     /locus_tag="PK674_01085"
     CDS             complement(7685..8122)
                     /locus_tag="PK674_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15159.1"
                     /translation="MENPVFFSASILSLQDAQTMTANHWDLAYKNEKNVLDVFVSDTI
                     KNINELSVSIIYDDQDVQIDLDNIKQQCDYEIVSQMGGSLILKFKNFNGKFDYGQSLF
                     ELPFGGKEPSILLSEGSVLLLNGNNKTLSIGSLNQNKDSHHGF"
     gene            8447..9526
                     /locus_tag="PK674_01090"
     CDS             8447..9526
                     /locus_tag="PK674_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOG15160.1"
                     /translation="MENPGIDIEKTLSLIAKNPNISDLHLSSGEYISYRLNGKIVKEE
                     SSGKISSEQMELFLKQLFYGNSQRFNKFLGDKESDFAYISKDKTIYRVNAYLKTGGIT
                     LVMRKVDKVAKKLEDIMLDSVSNSIKKNVLKKKKGLYLITGPTGNGKSTSLVAILEEI
                     NKNKSRILITIEDPIEYVFEPKKCIISQREIGKDTRSFANALKSAMRQDPDIVFVGEI
                     HDKETAEAVLNLAETGHLVFSTLHTNSASHTIKKFISFFPPNTQGSIAERLSESLIGI
                     QSQVLVKSMDDSARIGIFELMLNTQSIKTNIKKQDMNQINNIIKTSNTVGMITLKQYA
                     ETLIDKGIINPNDVEGILNKNSTKI"
     gene            9551..9961
                     /locus_tag="PK674_01095"
     CDS             9551..9961
                     /locus_tag="PK674_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15161.1"
                     /translation="MTNVELIKQKLPQELWEKASKFNIPDYFLENHPDAVVLVLNSRS
                     LSKDEEKQNWFNLVPMMNEEQVNKLKDILIRERDKIAEIEEKYEKKKEEIKEKYQTRF
                     DAVHYQKTMTSIQTKEDLIRDKEKEEAEDLLKNL"
     gene            9966..10865
                     /locus_tag="PK674_01100"
     CDS             9966..10865
                     /locus_tag="PK674_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15162.1"
                     /translation="MFLFKKNKKTLIVYILSFFFSISGFFLLDNAFGTDLMEQVFNPA
                     MSHDYIINIGKNKKTVGDTILREKTVIEVGGAPFQPACFINNVKQEGLNEQTCIQMGG
                     QRKTEIINVHSKAPLIVRITKFLLRMTIVLSISMIIFNSVKYMIEVLGGKDRKSAESK
                     KNLIYVVSGIMLALLSVSVINLIISIPKSSIKTSDDLSAFEIGCKTGSIILVGNDLKE
                     QICLKSTFGHPQGTMQFRERKYLPPFSMSADKMEAHTVGGYRCKICEGDGNSFDKNCM
                     RKRIYNSEMKTKCVEDLGGSIVN"
     gene            10890..11252
                     /locus_tag="PK674_01105"
     CDS             10890..11252
                     /locus_tag="PK674_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15163.1"
                     /translation="MMKLLLILFIFGFNLVNVFGLNINIPVPHGQEDIVVTGPTQIES
                     DESTIFDIIQIINDYLWFSIAGVAMVVLVYGGIKLITASGKEDSVSAANKMIFSALIA
                     IFVSILSYAIIKLVVNLF"
     gene            complement(11312..12292)
                     /locus_tag="PK674_01110"
     CDS             complement(11312..12292)
                     /locus_tag="PK674_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15164.1"
                     /translation="MLIEQRREEVAKEYIDNLKLQETDGVLKQIGDLLGLFKKEIEEK
                     SKLTKDVEKKLQDFLVSGGLFSEFKGFFSRLLLPSEIQKKLENAKEVLKNSTNKQEAR
                     EKLGLYVNKEPETTVLKTENLEGDAKGGVEQNLDNENLLTDSKEYLKNFSEAERYIIE
                     QAKKYGITDKNQIAYILATVKGESNFKNIKEFGGKNKRYGKDNYFGRGYVQLTHKSNY
                     RKFTNIIRKNKLQFKDNFGNNLDKEDLNLVKNPDSILKSNDLAAFILIHGMKEGLFTG
                     KKLSDFVNEHMVDFYNARNIVNGMSSSPGKFQNLAKKYLSQMEDKQKIIT"
     gene            complement(12328..12942)
                     /locus_tag="PK674_01115"
     CDS             complement(12328..12942)
                     /locus_tag="PK674_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15165.1"
                     /translation="MTGVESSGEKGFFDKISNGVKTVWEKAKELFGQIADWFKEMFGI
                     ESKTQKSLTELAKEIVENQTKDDLKKLIKDMEETTGLTEIEMKFIGRNKELSDYYKWL
                     KDNLSEKVAKKEMDIFLSNFIEKTKSDDKLTTDEEKQLKSKILTSDEKKELLGDKNLQ
                     KIIQEIVDSGEDYSVKEIIAVYDKNETKTKDEVLSKLSGEKDLD"
     gene            complement(13069..13476)
                     /locus_tag="PK674_01120"
     CDS             complement(13069..13476)
                     /locus_tag="PK674_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15166.1"
                     /translation="MEIFKRSIFGLVFLSLIFYLFYLFFNGIIVVNESFLDQNNIIIG
                     LYILIFLYYLVFYVIKPTYIKKFKLRNTLIGIFIIISSQSFFLNSGHEGLYFADIFTV
                     VGLFLTIIGPTNLLVSKKLKQEKLEKKMEIIEV"
     gene            13928..14554
                     /locus_tag="PK674_01125"
     CDS             13928..14554
                     /locus_tag="PK674_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15167.1"
                     /translation="MDKTFKKEWKEYLKNDEKQQYITNWEKLSTRFGSDILLSFFKNG
                     REHLGEIKNIRKEKSFSISFHEKIYRPLLEEKSLSYIKNKTKKPLMGLYSKYLHFLNI
                     YEIKKQEEIWKLMEEVEEMEQESEIIRQEGMLPIRKYEEDILSIDLECNILPPGHIPD
                     LKNLPPEEKIGNTIKTDKKIDKKTDEKTDTRGYKIIEGKGGQLKIDFD"
     gene            complement(14557..15678)
                     /locus_tag="PK674_01130"
     CDS             complement(14557..15678)
                     /locus_tag="PK674_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15168.1"
                     /translation="MNKILKKFLISFFITLFFYVLIYFIVFYYDYAGETILRFKKNIY
                     YLIFLFISIVGGLSLYGNENNKSKFIISIIFLVNFVYLIFIFSVGNIGLSRIQGLFLI
                     GFLLLGLVFSNLRNRVGNVVIIASVLGIFLTIFFTLIPLYGEGPDLEGFENNFETQLI
                     TYSKIGLHTNVAILEKDNKKYNLIKGIKNYDLAIGPSGSNITFKANNLYLNAFGYILF
                     KNKEILQIYPQTAIKINPNFEIEIITGMVKYYPQEIKSFSFTGKNIALLIVDQENIDI
                     ILGRYDDQLKNYIWEQNGKEIHQNKTILLISKNTLEFLNKIFPRQFKQNLENFDLFDK
                     YLEYNLEEKEDLKTFDQENIKKGIWQSVKDGLKFTEIIK"
     gene            16076..16579
                     /locus_tag="PK674_01135"
     CDS             16076..16579
                     /locus_tag="PK674_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15169.1"
                     /translation="MEEVIIVFLIEGLKETYPCLETVKIVPNIIKSATFQAKIHTNGK
                     KQIKIKTYTTNLNIFVCNGGHNVSSKEFEDTQNFNFSGFKIRIVDKNPVLMFGDEIIY
                     DFLNVPIQPNVIYWVSYKTKSHETLMNRPDVFCLLDGNLTNKLIFPRHKINKKYERKK
                     KNFNSIG"
     gene            complement(16621..17757)
                     /gene="hflX"
                     /locus_tag="PK674_01140"
     CDS             complement(16621..17757)
                     /gene="hflX"
                     /locus_tag="PK674_01140"
                     /inference="COORDINATES: protein motif:HMM:NF027678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="GTPase HflX"
                     /protein_id="HOG15170.1"
                     /translation="MLKVFLADIAPKNLSREILQDRMNELENLVDTFGGLVIVTKLQR
                     KEQPDFKTYVGKGKLEEIIQEMISVGANLLIVGNVLKPSQIFEINEQLRVASEQAGLK
                     EPIKARDRIDLILKIFEKHAQSTEARLQIELAAIKHMGPRIYGMGMELSRQGGSSGSG
                     GGATRGIGETNTERMKRHLKERQIKIKDKLKEFEKMRELHRQSRIKKGLPIIGIVGYT
                     NAGKSSLLNALTKKGVLAENKLFATLGTNVGKLFLFTDPEKGIGKEVLLNDTIGFIRD
                     LPPKLIDAFSSTLEDSIESVFLLHIIDASDPFIQERIKVVDEILDDIGAKQHRVLVFN
                     KIDLIDESRLKELKETYKNYDSVFISIIDEIGLEDLKDRIINLI"
     gene            17937..19013
                     /locus_tag="PK674_01145"
     CDS             17937..19013
                     /locus_tag="PK674_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15171.1"
                     /translation="MNYLFSVLLIIAVFFVGRYLFKKIKILDRPGADLKGVRGPVPTL
                     MGIFAYISFVLTMIWFFPGTSSNNMLRGLVLGVLPILIVELIEELGYMGKVKFRIPSI
                     VRLVSHIGGAIIAVYVGKIGVGQELILGSFVRHIPQYVFVVFFVVRAMLCINAINWID
                     GINAQASGVSAIGFLTIFLLIKFVVMRYYTEFNNRDILVLTSNMSLVLFFISLVSTVL
                     EYKPLGLLRDVGIMFFGFSLAYLSVLGGAKIGTLVVALSLVIFDAIWVGLYRILILKK
                     NPMKGDYTHIHHRLLGLKWTRPEVRAFVWIWSFVMMVLMLMQSANRLNKIIIFVMMAV
                     IFFGVNYYLFIVKKLKCGLNLKKD"
     gene            complement(19167..19958)
                     /locus_tag="PK674_01150"
     CDS             complement(19167..19958)
                     /locus_tag="PK674_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOG15172.1"
                     /translation="MINFRSLFFRKRRQKVLVISGGGFKGLYAVGVLKGLEELGIDQK
                     ITAIFGVSIGAVIGALWSHGISADRIFEILNSVSIDKFYTRNIFKKTGGILSNEKFRT
                     MMQEYLPNSFSSLQKKLYVGTVDANSAKFILFEKGKLQEIVLGSMAIPGVFPPVQYKK
                     YNLVDGGVLNNFPVDLAKKLYPRNKIIGIALHKFKKNQVIDTAVHNLMVNFEIILRSK
                     LLENTKYVDHLFHKDIPISILSLNKKKMKQAFDMGYNDCIKEFGR"
BASE COUNT         7389 a         2906 c         2302 g         7645 t
ORIGIN      
        1 atagatggaa tcaaaaaacc tcgttttgaa tatttatgag aaaaaattta agaaacacaa
       61 aaactaattt tttgaattcc ctatacaata aaaaatatct tactttattc atttccaatg
      121 aaatcaagtt atttatatga atactaataa tttatctagg caaaataacc cctaatccaa
      181 tatgcttaac aaagaattga tattatcaaa ttctcaagaa aatataatat ttaaatctga
      241 atttgacaat tcaagtcaca atttttgtaa tctttggatc aatacatctc tttgttcaat
      301 atcatctacc acaatatttc aatttaccac tcaattgttg taatttatct cataagaata
      361 aacttttcaa ttttgtaaat aatacttttg atatttttgt tgagatttat cagacatatc
      421 taccattctc gctttgtcta aatcaaaatt ttttccatat cttctaataa attcctcttg
      481 ttctctaaaa agatttttga agtatttgtc ctgtctatca aaatatctat caaactcatc
      541 aaaaacattt aaagtacgat cgattctata aggaccttga tagtaaataa ttccacgaga
      601 ttctatgttg gctatcctgt tttgataata aataacaaga gaaatgataa agacaacaaa
      661 tatcacaatt atccatcaac taaatcaaag atacttatta acttttgcaa agtttctctt
      721 aatagaatta tcacaattct tgaccgtgct tttcaaagtt ttttctgatt ttttcatttt
      781 ctctttttta ccatttaaaa aagaactaga ttaatctagc tcgcaaacaa aaacattatt
      841 tatcaaattt agatatctca ttttcaatct ttttaatatc caaatcaata ctttcgatca
      901 tatcttccct tcactcattt ttgaattttt tcttataatc tttgagcttt tgaagagttg
      961 atttgagatg ttttaatgtt tcatctttgt gttgcttttg atagtcaatt ttttttaatt
     1021 ctgattcata tttattgaga tcaatcagat ttcacccagc ataattgtct tgctcttcat
     1081 cgtatcaatc atagattagt cacagtcttc tatcaatgag attatcaaaa taattctgta
     1141 aatttgccca atctcacaat cataatatcg gagatcttgg cgtgtcagaa aaactttcta
     1201 atcatgaaac ctcattgttt tgaacaaatg attggaaatc ttgagaatta tcaaattctc
     1261 tttcaatttt ctcctctttt caattaatgt ttttgtaaac ataaactttc cctttgaaca
     1321 tgtttgatat tggttaaaaa ataaaaagta gcaccaaaac tatatcggtg ctataataat
     1381 aatttttttt tatttttcaa gtttttttac ctttttgcct gctaaatggt ttgacttatt
     1441 tatatttatc caagtttata tacataaatt aaaaattatc ctatactttt ttttataaaa
     1501 taacccattc aataaacttt taaaaacata gtttgcttaa aaagtaaaac tttttaaacg
     1561 aattttttat tattttaaaa aacattgcag tttttttcta aaaggttata catttcatac
     1621 tttatacaat ttcaatacgc tatgaaaaaa caaagtttca ttaaaattga atgatcagct
     1681 tctgttggta caaaatgaca aatagtaatt ccaaaaaatg ttagagacaa atttaaatta
     1741 aaattcggaa atgatttaat tgtactatct tctgataatg ctatattgtt ggtaaaatca
     1801 gaaagtctag aaaatatgat atgatctttt gaaaatataa taaatgattt ataaaaaact
     1861 acttactttc ctgtaacttt ttccataaaa cctcttgttc tttactcaat ctctttggga
     1921 ttttaagttt tggaactacg aataagtctc atttttttgc aaaaattccc ccactaccaa
     1981 aaccttttcc tgaaattttt atttgctcat ccaattgtgt tcatttagga atttttattc
     2041 taatttttcc acttggatgt agaatttcat actctcatca taaaaccaaa tcataaatcg
     2101 taacttcctg atttacataa atatgatctc attttctaat ataatttgga ttctcgctta
     2161 cattaatttg tatgtataaa tctcctgttg gcataccgtt ttgtccatca tctcatttat
     2221 tggaatattt caaataagct ccatggttta ttcatgctgg aatcttcact tccaaaattt
     2281 cctttacttc ttccaatcac ccgttttcta atttttttca atctttaaag tattgttgtc
     2341 agcttcatcc acaggtggga caagtacttt gactttgcat caccccaaaa ggagactgaa
     2401 cttgtttagt tactctccct ctaccagaac acatatcaca agtttttgct tctgctcatt
     2461 ctaccactct tttacgagta taagatattt tttttgtaac tcataaatac gattcatcaa
     2521 aagtgattgt aattttcttt ttgagatctt ctcaacttct gggtcatcca cctcatccaa
     2581 aactaccaaa tcatcatcaa aaaatatttc aaacaatatc tcataaatca ccaaaatcaa
     2641 aacttgctca tcaagtttgg aatcatcaaa aatttcatca tccaaaacca ccaaatcatc
     2701 actttctaaa catatcatat tgttgtcttt tttgtttatc tcaaagtatc tgatgagctt
     2761 catttacttc ttgaaatttt tctttgtttc atcatgcctt gtccggatga tattttacag
     2821 ccgcttttct aaaggccttt ttgatttcgt cttcagtagc gtcttctgac actcacaata
     2881 tttcataata atttttttta ggatcaaaat ccataactat aaattaaaaa tttaaatata
     2941 atttacagaa aagcacagat tattctaaaa aacagtagca ctaaaacaag cgttgtgcta
     3001 tactaataaa aaaaatgcaa aatgcaaact attttaaaaa cattacatca gtactttcta
     3061 attgatgatg aatttataaa cattatcctt ttatcttcat tttattaatt ttaaattcca
     3121 gttcctccac ttctttttgt aaattttgaa tatcttgttt tttcttttct ataatttcca
     3181 tagatgctgt tgaaagaatg gagcttatca aagatttcat atgttgtaaa tattcttgtt
     3241 tgttttttaa atcttcttcc atttccatca acacattttt agttactcca atctgttctg
     3301 agacactttt ggctcaaata tttatatcaa ttacattatc catttcataa tctgatggtg
     3361 ttttttcata atctttaaca taaactatat ttctaatgtt taacaaggct tccaatagat
     3421 cttctctttc tgataaaaaa tttataaact ccaaattagc ttgaacgaac acatctactg
     3481 tttcatgttt tttaactcat aaattttgtt ttattttttt gagcttatct acaatatcca
     3541 taaaaatatt cattctataa tttttttctt tcaaattaaa atctcatata gtgcttatat
     3601 caaaattcga tattttaagc ttgttaaaaa atagcttttc tatttcctgc ataaataatg
     3661 gcatcaatgg tcttaaaatc tccaaagaaa cgataaaaga aaacaaagct acatgcggag
     3721 tgctctcata ctcataaatt ttagtagact ccaaataatt ttcacaaaga tcattgataa
     3781 taaattttaa aattttagac ccaaaatcca atatgttttt atttttgttt tttaattctt
     3841 ctagcagaga tttgagtcaa tgcaaaatcc agttatcata atcacttatg tcctgttcta
     3901 tcaaattgaa aagctcttta atgtttattt ttgaaattca attaatgtag ttgttgtaaa
     3961 tatatctgta tgcattccaa actttattca acataaaatg tatatattca atattattag
     4021 tatccaatat gactttgttg ttttcttttt gtcataacaa caaaagtaat cttagcaagt
     4081 ctgaattgaa caaatcaagt attccagaaa gttcttttgt taaaaattta gagttgtaat
     4141 ttgtaataat atttgattct tggtcagtca aataaggaat aaatagcgaa tctgaaaaaa
     4201 atagtgtttt ggaataatcc aatcacagta gtaacaaatt tacaaaaaat tctttatcat
     4261 cttttgttca aataaatata tttcacaagt tttttatacc agaatatccc tccatacttt
     4321 ttcaaaggtt atttttaaac aacaaccata cagtatccaa aaaatcttta ttaaaactat
     4381 cttgtttttg caaaaccaat ggttcttgag atcaaaaaat agcagaaaaa tctaaactaa
     4441 agcgttctca atcagacttt attgcaaaac tgtctcctaa aagttccaaa agaatcatta
     4501 ttttttcaga atctctttct atttttcaca aaagcctttt aatacccttc agtcagtttt
     4561 ttaaactaga tctttcagta ttttcataaa tatctatata ttttgaaagc ttgcttacgc
     4621 caccagaaaa atcaaaagaa aacaaaacat caacaagctc ctctatgcta aaaacatcat
     4681 ttaattgatt atccaaaacc aaattgagta ttatagcggt tagtgtgaga tcttttctgg
     4741 actttgcaat tttatactgc tctaccaaaa tatcatcatt tatcaaatat ttttgtcatt
     4801 tttcagaata caaaaccggt attaaggttc cctttgcaga tttgttggaa atattaacaa
     4861 aatttaaatc tttgagataa tccaaaatgt ggttttctac atcaaatcac tttattgtaa
     4921 tatcttttgt ctcaaaattt tgaagcaaaa aatctaaaga gtaatcatat ttaatacccc
     4981 actgatccat agtaatttta tatagttctt ccccagattt agcacaatac ataagctctt
     5041 cttccgtttc atttatctgt tctaaatttc atatatcatt tatataagtt tcaacatttt
     5101 caataaaaac atccacaggt ttaccaacaa aatctccaac atgtttagta aaatttccat
     5161 caacatctat tgcatatata tttgttggta aattgtgttt ttgtgcaatt tcaagaccat
     5221 attcatcatg tcatggtgta attctaacca ctcaagttca ctcaaaagaa accacactat
     5281 catctcatat gataggtatg tttttattta ttattggaat aattacattt tgtcatatca
     5341 attttttata tcttttatct tgtggattta cagctacagc cacatctgca aaaatagttt
     5401 tgagattttt cgtaggaaca ctaatagaaa ctcatttaga ttctataaaa tatttaataa
     5461 caaattcctt ttttttcttt tttttaataa acagattggt cttcaataca tttgtctgta
     5521 attctttgct tcgtgaaact accttttttt ctaaatgaat ttttcaatct ttatataaat
     5581 taacaaaaac ttctctaaga aaaatataaa atttttcatc tacaacaaaa tgattttcgt
     5641 ttataaaaaa agaaaatcca agtttttcca aaaattttat gttctgcttc acttgttttt
     5701 taagatctaa ttgtaattgt tttaaattaa cattatttct atttaatttg gaataccaat
     5761 ttgatgacaa agcaaagttt atatcaaaat caagcccatc agaaccccat aagctcattt
     5821 tagaaatata gtctttcata aaaattaaaa acaaagctcc aggatgaacc ttttgatcag
     5881 cagacaaagc tcttgaataa ataaattttg atgagggaaa agatgatctt tgtatgtttt
     5941 tatttttatt tatatatttc tttggaaaca tagacataat tataaaaaat tgaaacaata
     6001 aagcacaatt taaaaaatgg acaaaaaata atcgttaata tttttgagtc ctatataatt
     6061 tatatactgc atacaaaaac aaactaacta taataatcag taaggtatct tcaaacggtc
     6121 aaacttgaac tttttgtgct tggtctatga gaacactagt tccatcagaa caaattgctt
     6181 gcacttgata atatgcatac tgatcttttt ctgcatattt atcaaaaaaa tactcaaaac
     6241 tagcttctgt cgtttctcaa accaactttt tatctgacat atcttcccaa tctgatctat
     6301 atatttcgta cttagtggcg ttttttacct catcccatac caaaaaatat ttatctcata
     6361 ttttttcaga ctttaccact attcactgaa caacacaaga agctttaact ttttgtggat
     6421 tatattctac aatacttgaa gggtctcatg atatattaga caattcatcc aaaggagaaa
     6481 tttggaaata ataagttgta tcagtctgca aattttctat caaaactcca gtagcgttta
     6541 tatctaccgt tttttgtaag ttattttggt cagtaccata aaaaactcta tatttaggaa
     6601 aatgtcatct agaatcccaa gaaactatta ctgatgtccc atccactcaa gtagaaacaa
     6661 atcttacatt cccaacaccc ttgttttctt tcacaaaaat acttgcaaca ttgtcgtagt
     6721 ttctcggtat tcatgcatac atcaaaacaa cagaaatatt tttattaccc aaaatatctg
     6781 tcataattat atctttttta aaaattcctg gagaaagaag atccaaagga tagctggtgc
     6841 catcatcaaa tatcatctga gctgagctta cttcatctac agcagaaaca gtaaatgtaa
     6901 ctttatctca ttcctttaag actcaagaag gtattatttc tatggaatta aacactcaat
     6961 caaatggggg ttgataggtt actgtcacca cattagattc tcacaataaa acattattta
     7021 tatttacagc ttttatttga atagtgtttg gtccagagtt taaatttcaa acatatacat
     7081 taaaatttca cattgaatct gtttgtcaac tggtaactaa catatcattt aaataaacat
     7141 ctatcggtga attccttaaa tcaaagctag atcaaattat atttatagta gatttttttt
     7201 ctatagaaga atctatagga gaagttatat ttatttgttt caagctcgaa tctgaactag
     7261 catttcatac aacaaccgtc gctgttcagt gaaattcgta atttaaatcc gtaacatcag
     7321 atacttttat ttgatatgta cctgcttttt taatttctaa tcattttgaa aaggtctttg
     7381 ttcactgatc ttggggtaaa aaagaataaa atcccatact aggaacaaca taatcatccg
     7441 gcaacaatca atttatttca aacaaaacat ctcaaatata atcttttaat atttcaccat
     7501 ttttgagtgc tgttattgta atatctactg gctgtcaaac tcaaaaagtg tctggttcta
     7561 cctttacaga aaaagaatct ggctcctctg ccaaagaaac caatggaact agggctgaaa
     7621 aaaacataaa cactcaaacg gctaattttt tataaaattt attcatctac atattggttt
     7681 tttattaaaa tccatgatgt gaatctttgt tttgattcag cgatcatatt gataatgttt
     7741 tattatttca atttagtaat aaaacacttc actcactcag caatattgat ggttctttac
     7801 ctcaaaaggg taattcaaat aggctctgtc cataatcaaa ttttcaatta aaatttttaa
     7861 attttaaaat caaacttcat cccatttgag atactatttc ataatcacac tgttgtttga
     7921 tattatctaa atctatctga acatcttgat catcataaat aattgaaaca gaaagctcgt
     7981 ttatattttt tatagtatcc gaaacaaaaa catccaaaac atttttctcg tttttgtatg
     8041 ctaaatccca atgatttgct gtcatagtct gagcgtcttg tagtgataaa atactggcag
     8101 aaaaaaaaac agggttttcc ataaaataaa acaaaataag cgaaaatagt attgccacca
     8161 aaaagattac tgtgtttgta cggagcttct ttgttctcat aaaaaaattg attacaagtc
     8221 aagtaaaagt ttctatgtat tttatataat aatgtaaagt aaaaaagcaa acaaaaaagc
     8281 aaaaaatata aattaagttc attgacctaa aatttacaaa aattattttt aaaaactatt
     8341 ttgatacaaa tgtcaaacta aacacaaaat taaagcccga tttttttgac aaaaataatg
     8401 ttttttatta taataatacc gtataattta taatataaat ttaaatatgg aaaatccttg
     8461 aatagatatt gaaaaaacat tatcattgat agccaaaaat ccaaatattt cagatttaca
     8521 tttatctagt ggagaatata tttcttatag attaaactga aagattgtta aggaagaatc
     8581 ttctggtaaa ataagctctg aacaaatgga attattttta aaacaattat tttattgaaa
     8641 ttctcaaaga tttaataagt ttttatgaga caaagaatcg gattttgcct acatatccaa
     8701 agataaaacg atttatagag taaatgctta tctaaaaact ggaggaatta ctcttgttat
     8761 gagaaaagtc gacaaagtag ccaaaaaatt ggaagatatt atgttggata gtgtttcaaa
     8821 tagtattaaa aaaaatgttt taaaaaagaa aaaagggtta tatttgatta cttgacctac
     8881 tggtaattga aaatctacta gtttagttgc tatactagaa gaaatcaata aaaataaatc
     8941 tagaatttta ataacaatag aggatcctat agaatatgtt tttgaaccaa aaaaatgtat
     9001 tatatcacaa agagaaatct gaaaagatac tcggtctttt gcaaatgcat taaaatcagc
     9061 aatgaggcaa gatcctgaca tagtttttgt ctgagaaatt catgacaaag aaactgcaga
     9121 agctgtgcta aatttagcag aaactggaca tctggttttt agtactttac acacaaattc
     9181 tgcctctcat acaataaaaa aatttatatc tttttttcca ccaaatactc aatgaagcat
     9241 tgctgaaaga ctttctgaat ctttaatcgg aattcagtct caagttttag ttaaaagtat
     9301 ggatgattca gccagaatat gaatatttga gttaatgttg aatactcaat ctataaaaac
     9361 aaatattaaa aagcaagata tgaaccaaat aaataacatt ataaaaacct ctaatacggt
     9421 ttgaatgata actttaaaac aatatgcaga aactttgata gataaaggta ttattaaccc
     9481 aaatgatgtt gaatgaattt tgaataaaaa ttcaacaaaa atataaattt ataaactaac
     9541 aatcaaaaaa atgactaatg tagaacttat aaaacaaaaa ctacctcaag aattatggga
     9601 aaaggcatca aaatttaata ttccagatta ttttttggaa aaccatcctg atgctgtagt
     9661 cttggtatta aactctaggt ctttatcgaa agacgaagaa aaacaaaatt ggtttaattt
     9721 agtccctatg atgaacgaag aacaggtaaa taagctaaaa gatattctta ttagagaaag
     9781 agacaaaata gctgaaattg aagaaaaata tgaaaagaaa aaagaagaaa tcaaagaaaa
     9841 atatcaaact agatttgatg ccgtacacta ccaaaaaacg atgactagta tacaaaccaa
     9901 agaagacctt attagagata aagaaaaaga agaggctgaa gatttactta aaaaccttta
     9961 ataaaatgtt tttatttaaa aaaaataaaa aaacattaat tgtttatatt ctaagtttct
    10021 ttttttctat aagtgggttt tttttattag ataacgcttt ctgaacagac ttaatggaac
    10081 aggtattcaa tcccgctatg agtcatgatt atataataaa tatatgaaaa aataaaaaaa
    10141 cagtttgaga tactattctt agagaaaaaa cagttataga agtatgaggt gctcctttcc
    10201 aaccagcttg ttttataaat aatgtgaaac aagaaggttt aaacgaacaa acttgtattc
    10261 aaatgggttg acaacgaaaa actgaaataa taaatgttca ttccaaagca ccattgatag
    10321 ttagaattac taagttttta cttagaatga ctattgtcct ttctatttct atgataatat
    10381 ttaattctgt aaaatatatg atagaagttt tgtgatgaaa agaccgaaaa tcagctgaat
    10441 ccaaaaaaaa tttaatttat gttgtttcct gaattatgtt agcattatta agtgtaagtg
    10501 taattaattt aataatatcc atcccaaaaa gtagcataaa aactagtgat gacctctctg
    10561 catttgaaat ttgatgtaaa acatgatcaa taatattggt tggtaatgat ttaaaagaac
    10621 aaatttgttt aaaatctact tttggacatc ctcaatgaac aatgcagttt cgtgaacgaa
    10681 aatatctacc acctttttct atgtccgcag ataaaatgga agctcatacg gtatgatgat
    10741 ataggtgtaa aatatgtgaa tgagattgaa atagttttga caagaattgt atgcgaaaaa
    10801 gaatatataa ttctgaaatg aaaactaaat gtgttgaaga tttaggatga tctatagtaa
    10861 attaatattt ataattataa aaaaagtcta tgatgaaatt attgttaata ttgtttatat
    10921 ttggttttaa tttggtaaat gttttttgac ttaatataaa catacccgtt ccacatggac
    10981 aagaagatat agtggtcacc tgacccacgc aaatagaaag tgatgaaagt acaatttttg
    11041 atataataca gataataaac gattatcttt ggttttctat agctggagta gctatggttg
    11101 ttttagttta ttgatgaata aaattaatta ctgctagttg aaaagaggat tctgtgtctg
    11161 cagcaaataa aatgatattt tcagctctta tagcaatttt cgtatctata ttatcatacg
    11221 caataataaa gctagttgta aacttatttt aattaaaaca aaaaaataaa catttagaca
    11281 agttactaaa ataacaaaac aataaaactt actatgttat aattttttgt ttatcttcca
    11341 tttgagataa atattttttt gctaaatttt gaaatttgcc tggagaacta ctcattcagt
    11401 taacaatatt tcttgcgtta taaaaatcta ccatatgttc gttaacaaaa tctgataatt
    11461 tttttcatgt aaacaatcat tctttcattc aatgaattaa aataaaagca gctaaatcat
    11521 tacttttaag aatagaatcc gggtttttca ccaaattcaa atcttcttta tcaagattgt
    11581 ttcaaaaatt atctttaaat tgtagtttat tttttcttat aatatttgta aatttcctat
    11641 aattactttt gtgtgtaagt tgaacataac ctctaccaaa ataattatct tttcaatatc
    11701 gtttattttt tcatcaaaat tctttgatat ttttgaaatt actttctcat tttactgttg
    11761 ctaaaatata ggctatctgg tttttatcag taattcaata ttttttagct tgttctataa
    11821 tatatctctc tgcttcagaa aaatttttta aatattcttt actatctgtt aataagtttt
    11881 cattatcaag attttgttca acacctcact tagcatctca ttcaagattt tctgttttca
    11941 atacagtagt ttctggttct ttgttaacat ataatcataa tttttctcta gcttcttgtt
    12001 tatttgtact atttttaagt acctcctttg cattttctaa ttttttctga atttctgagg
    12061 gtaataatag tcttgaaaaa aatcatttaa attcagaaaa taatcaacca gaaactaaaa
    12121 aatcttgcaa ttttttttct acatcttttg ttaacttact tttttcttct atttcttttt
    12181 taaataatca caataaatca cctatttgtt tcaaaactca atctgtttcc tgtaacttta
    12241 aattgtctat atactctttt gctacttctt ctcttcgttg ctctatcaac atcttttact
    12301 caaataaata aattattata ttaaatctta atccaaatcc ttttctccag atagtttact
    12361 caaaacctca tccttcgttt ttgtttcatt tttatcataa acagcaatta tttctttaac
    12421 actataatct tctcaactat caactatctc ttgaataatt ttttgaagat ttttatctcc
    12481 taataactcc ttcttttcat cgctagtcaa aatttttgat tttaattgtt tttcttcatc
    12541 tgtagttaat ttatcatcac tttttgtttt ttcaataaaa ttagataaaa atatatccat
    12601 ttcttttttc gcaaccttct ctgacaaatt gtctttcaac catttataat aatcagacaa
    12661 ctctttattt cttcaaataa atttcatctc aatttctgtt aatcaagtgg tctcttccat
    12721 atctttaatt aattttttaa gatcatcttt tgtttgattt tctacaattt ccttagctaa
    12781 ttctgttaaa cttttttgcg ttttgctttc aattcaaaac atctctttaa accaatcagc
    12841 aatctgtcaa aataattctt ttgctttctc ccatacagtt ttgactcaat tcgatatttt
    12901 atcaaaaaat cctttttcac cagaactttc aactcatgtc atttttatta ttttattaaa
    12961 taaatataat acaaattaat tataaatatt tttttaaaaa aagcaaaaaa atccagcact
    13021 tttttactga attttttgta catcttacca tattttcaca aaaatacatt aaacctctat
    13081 aatttccatc tttttttcta atttttcttg ttttagtttt ttggaaacaa gtaaatttgt
    13141 tggtcaaatt atagttaaaa ataatcaaac tactgtaaat atatcagcaa aatacaatca
    13201 ttcatgtcca gaatttaaaa agaaagattg actggatata ataataaata ttcaaattaa
    13261 agtatttcgt aacttaaatt ttttgatata agttggttta attacataaa aaactaaata
    13321 atataaaaat atcaaaatat aaagtcaaat tataatatta ttttgatcta aaaagctttc
    13381 atttaccaca attattcaat tgaaaaacaa ataaaacaaa taaaaaatta atgacaaaaa
    13441 aaccaaacca aaaatagatc ttttaaatat ttccattttc tttataaaaa aataaattat
    13501 tatttttaat ataagaattg tttttttctt ttcaacaaat aatttgttat ttatatatta
    13561 aatattaaat tttaaaatat aaatttaaaa aattaataac aataataata tatattttat
    13621 aaaaaattgt tgaaatttgt tttactacat tataactata ttttattaaa aagcttttgt
    13681 gtaaaaaaaa tgttgaattg taaaaaatat acaaaactaa ataaaaagta tttataatat
    13741 tttgttgaaa agttaaaaaa atgttagaaa ctttttaatt aaaaaacaat aatataaagt
    13801 agatttttaa ttaaaatgtg gattgtattt cttgtatttc aaaataaaaa tagcacttga
    13861 caaaataaaa gaaattatta taataaacac tgttagttaa ttttttttaa attattaata
    13921 aattgaaatg gataaaacct ttaaaaaaga atggaaagaa tatttaaaaa atgatgaaaa
    13981 acaacaatac attactaatt gggaaaaatt atcaacaaga ttttgaagtg atattttatt
    14041 aagttttttt aaaaattgaa gagaacattt aggagaaata aaaaacattc gaaaagagaa
    14101 aagtttttcc atctcttttc atgaaaaaat atatcgtcca ttattagaag aaaaatcttt
    14161 aagttatata aagaataaaa caaaaaaacc attaatgtga ttatatagta aatatcttca
    14221 ttttttaaat atatatgaaa tcaagaaaca agaagaaata tggaaactaa tggaagaagt
    14281 agaagagatg gaacaagaat cagaaattat tagacaagaa tgaatgctcc caattcgaaa
    14341 atatgaggaa gatatcttat ctattgattt ggagtgtaat attttacctc cttgacatat
    14401 tccagactta aaaaatttac ctccagaaga aaaaatttga aatacaataa aaacagacaa
    14461 aaaaatagat aaaaaaacag atgaaaaaac agatacaaga ggatataaaa ttattgaagg
    14521 aaagggttga cagttgaaaa tagactttga ctaagtttat ttgataattt ctgtaaattt
    14581 taatcaatct tttacacttt gccatattca tttttttata ttttcttgat caaaggtttt
    14641 tagatcttct ttttcttcta agttatattc caaatattta tcaaacaaat caaaattttc
    14701 taaattttgt ttaaattgtc ttgggaaaat tttattcaaa aattctaaag tattttttga
    14761 aattaataaa atcgttttat tctggtgaat ttcttttcca ttttgttccc aaatataatt
    14821 ttttaactga tcgtcgtatc gccccaaaat aatatcaata ttttcttgat ccacaatcaa
    14881 taaagctata ttttttccag taaaagaaaa acttttaatt tcttgtggat aatattttac
    14941 cattccagtt attatttcta tttcgaaatt agggtttatt ttaatagctg tttgaggata
    15001 tatttgtaat atttctttat ttttaaataa aatatatcaa aaagcattta aatataaatt
    15061 atttgcttta aatgttatat ttgatcatga tggtcaaata gccaaatcat aatttttaat
    15121 tcattttatt aaattatatt ttttgttatc tttttctaaa atagctacat tagtatgtaa
    15181 acctattttg gaatatgtaa taagttgtgt ttcaaaatta ttttcaaatc cttcaagatc
    15241 tggtcattct caatataaag gaatgagtgt aaaaaatatt gttaaaaaaa ttcataatac
    15301 agaggcaata attacaacat ttcaaactcg atttcttaaa ttactaaaaa ccaatcacaa
    15361 taataaaaat catatcaaaa acaatcattg aattcggctt aatcaaatat ttcatacact
    15421 aaaaataaaa attaaatata caaaattgac taaaaatatt atagaaataa taaattttga
    15481 tttattattt tcgtttccgt ataaacttaa acctcaaaca atggaaataa aaagaaaaat
    15541 taaataataa atattttttt tgaatcgtaa aatagtttct caagcatagt cgtaataaaa
    15601 tacaataaaa tatattaata catagaaaaa taaagttata aaaaaagaga ttaaaaattt
    15661 tttaagtatt ttattcatcc aataaaaatt ttaatataaa ttctaattct gataataaat
    15721 aaataaattt gattttcaat aaattcggtt ttaaatttaa taaggaaact tttttcacaa
    15781 ctcaaaattc tataatttaa tttaaagtgt cttgaaaaat caaatttatt acccattttg
    15841 tagataaaat atatttatat ttcgctcaat ttcgttaata tttcatacat tctccatatt
    15901 ttaatcaaca aaattttgct tatatttttg ttataattag atttataaga gaacatatct
    15961 cgcttcaact aaaatccaat attaaaacta aaattaaaaa acggttgatt tacgaaaaag
    16021 aatttatatt aaaaaatccg tactgtaata aaaaataaaa ctaaaattca aaaaaatgga
    16081 agaagtaatt attgtttttt taattgaagg gttaaaggag acataccctt gtttagaaac
    16141 agtgaaaatt gtccccaata ttattaaatc agccactttc caggctaaaa ttcatacaaa
    16201 tggaaagaaa caaataaaaa taaaaactta taccaccaat ttaaacattt ttgtttgtaa
    16261 tggtggacat aatgtatcat caaaggaatt tgaagatacc caaaatttta atttttcggg
    16321 ctttaaaatc aggatagtag ataaaaatcc tgttttaatg ttcggagacg aaattattta
    16381 tgattttttg aatgtcccta tacagcccaa tgttatttat tgggtttcat acaaaacaaa
    16441 aagtcatgaa acgctcatga atagacccga tgttttttgt ttattagatg ggaatttaac
    16501 aaacaaatta atattcccaa gacataaaat aaacaaaaaa tatgaaagaa agaaaaaaaa
    16561 ttttaattct atatgatagt tatggttgtt gaatatttca acaaccattt ttttaaacac
    16621 ttatattaga ttgataattc tatcttttag gtcttccagc cctatttcat caataattga
    16681 aataaacaca gaatcataat ttttgtaagt ttctttaagt tctttaagtc tactttcgtc
    16741 tattaaatca attttattga aaactagaac tctatgttgt ttagctccga tatcatctag
    16801 aatttcatct accaccttta ttctttcttg aataaatgga tcagaagcat ctatgatatg
    16861 cagtaaaaaa acactttcta tactatcttc caaagtactt gaaaaagcat caataagttt
    16921 tggaggtaga tcacgaataa atcaaattgt atcgtttaat agaacttctt ttcaaattca
    16981 cttttctgga tctgtaaaaa gaaacaattt tcatacattt gttcaaagtg tagcaaaaag
    17041 tttattttca gccaaaactc attttttagt tagagcattc aataaggatg acttaccagc
    17101 atttgtataa ccaactattc aaataattgg taatcacttt ttgattctgc tttgtctatg
    17161 taattctctc attttttcaa actcttttaa tttatctttg attttaattt gcctttcctt
    17221 caagtgtctt ttcattcttt ctgtattagt ttctcagatt catctagttg ctcatcatca
    17281 gcttcagcta cttcatcatt gacgagaaag ttccattccc attccgtaaa ttcttggccc
    17341 catatgctta attgctgcta gctctatttg taatctagct tctgtacttt gagcgtgctt
    17401 ttcaaaaatc ttcaaaatca aatctattct atctcgtgct tttataggct cttttaatca
    17461 ggcctgttca cttgcaacac gaagttgttc atttatttca aatatttgag aaggttttaa
    17521 aacatttcat acaatcaata aatttgctca aacactaatc atttcttgga taatttcttc
    17581 caattttcat tttcaaacat atgttttgaa atctggttgt tcctttcttt ggagttttgt
    17641 cacaatcacc aatcatcaaa aagtgtctac tagattttct agttcattca ttctgtcttg
    17701 taaaatctct cttgaaagat tttttggagc tatatcagct aaaaatactt ttaacataca
    17761 atgagttatt tatagtttaa aagtttgtaa gttgacaaag taagaccatt gttccaatta
    17821 gaaacatatc accaattatg ttgttttcta aattgttttc aagtacttta cattttgatt
    17881 tgaaaaaaac aatatattca atatgtttta tttagtttat tttttaaata aaatcaatga
    17941 actatttatt ttctgttcta ctcattatag cagtattttt tgtttgacga tatttgttca
    18001 aaaaaatcaa aattttagac agacctggtg cagatttgaa atgagttaga tgaccggttc
    18061 ctacacttat gtgaattttt gcatacatca gtttcgttct tacaatgatt tggttttttc
    18121 cagggacaag ttcaaataat atgcttcgag gtcttgtgct atgagtcctt ccaattttaa
    18181 tagtcgaatt aatagaagaa ttgtgatata tgtgaaaagt aaaatttaga attccttcga
    18241 ttgtcagatt agtatcccat atatgatgag caattattgc tgtctatgtt tgaaaaatcg
    18301 gagtttgaca agaactaatt ctttgatctt ttgttcgaca tatcccacaa tatgtttttg
    18361 tagtattttt tgtagtacga gcaatgcttt gtataaatgc tataaattgg attgattgaa
    18421 taaacgcaca agctagctga gttagtgcaa tatgattctt gaccattttc ttgttaataa
    18481 aattcgttgt catgagatat tataccgaat tcaataatcg agatatctta gttcttacat
    18541 ccaacatgtc tttggtttta tttttcataa gtttagtgtc tactgtatta gaatataaac
    18601 cattatgact tctcagagat gtatgaatta tgttttttgg atttagtttg gcttatttat
    18661 cggttctatg atgagcaaaa atatgaacat tggtagtagc tttgtctctt gtgatttttg
    18721 atgcaatatg ggtatgatta tataggattt tgattctcaa aaaaaatccc atgaaatgag
    18781 attatacaca catacaccac agacttttat gactaaaatg gactagacca gaagtaagag
    18841 cttttgtttg gatttggtcg tttgtaatga tggttttgat gctgatgcaa tcagcaaata
    18901 gattaaataa aataataata tttgtcatga tggcagtaat ttttttctga gttaactatt
    18961 accttttcat agtaaaaaag ctaaagtgtg gtttgaattt gaaaaaagat tagggaataa
    19021 tcgttggcaa gtttgtaaag ttcttaaagg cccccctttt ttaagggggg aaagggggga
    19081 ttttatgata aatgagggtt ttatttaatc ccccttaatc ccccttaaaa agggggacta
    19141 ccatcaactt tactaacttt gcttgtctat cttccgaact ctttaataca atcattatat
    19201 cacatatcaa aagcttgttt cattttcttt ttatttaaag atagtattga aattggaata
    19261 tctttgtgga ataaatgatc tacatatttt gtattttcta atagttttga cctcaaaata
    19321 atttcaaaat ttaccattaa gttgtgaaca gcagtatcta ttacttggtt ttttttgaat
    19381 ttatgtaagg ctattcatat tattttattc cttggataaa gctttttggc taaatctaca
    19441 ggaaaattgt ttaatactca tcagtcaact aaattatatt ttttgtactg tactggtggg
    19501 aaaacaccag gtattgccat gcttcataaa actatttctt gtagttttca tttttcaaat
    19561 agaataaatt tagcactatt tgcatctaca gttccgacat atagcttttt ttgtagagaa
    19621 gaaaagctgt ttggtaagta ttcttgcatc atagtcctaa atttttcgtt agacaaaatt
    19681 catcatgttt ttttgaaaat atttctagta taaaacttat caatagaaac actatttaat
    19741 atctcaaaaa tcctatcagc actgattcca tgcgaccata atgctcagat aacagctcct
    19801 atacttacac caaaaattgc ggtaattttt tgatctattc ataactcctc tagtcatttc
    19861 aaaactcaaa cagcatataa tcatttgaat cctcatccag atattacaag aactttttgg
    19921 cgtctttttc taaaaaatag cgatctaaaa tttatcataa ttgatattta tataaaaata
    19981 caacaaatat tttatttttt tatttatttt tttcaagtgt tcataaatgt gtttgtcgtt
    20041 cttgagtcct ctcgtagtac tatgggaggc agaaagaaca tgactagtag aaacggggat
    20101 tattcttttt cttgagtcaa gcaattctgt gtggtggaag acactaaatc cctctaaaaa
    20161 gagcagctcc ctttgaaaaa gggagataat aatgttgtga caaaagttcc tcccttgggg
    20221 gaggttgcaa tccccgattc at
//
LOCUS       DAOSID010000009        17477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_3466_length_17477_cov_11.149122, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000009 DAOSID010000000
VERSION     DAOSID010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 17477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17477
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3466_length_17477_cov_11.149122"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..844
                     /locus_tag="PK674_01155"
     CDS             71..844
                     /locus_tag="PK674_01155"
                     /inference="COORDINATES: protein motif:HMM:NF023762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="helicase-related protein"
                     /protein_id="HOG15173.1"
                     /translation="MTKHPNLKDFLPKNKDSKPLPFIDHLLSKLDEHISKNGKILILT
                     LTKKSSEEITNYLVSKGYKAYYLHSEIVTMDRWEILKKLKTGEIDIIVGVNLLREGID
                     LPEVSFLAILDADKEGFLRSSTSLIQIIGRASRNPNSEVVLYADQFTESMIKSLRETY
                     RRRKIQDEFNKKYNITPKKATSNIKDLESVRTDQDLGQHFSAITKGKVKRLKRMTKKE
                     KEFISKNLKKQMDLAIKEWRFEDATVIRDQIKELEEGIV"
     gene            complement(979..1464)
                     /locus_tag="PK674_01160"
     CDS             complement(979..1464)
                     /locus_tag="PK674_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15174.1"
                     /translation="MIKKNIALLSVIIAGFSTIAIAQVGFEVIPETESSASAVGAVVD
                     NIREGGSVWKKYKDTAYGPENIVDGKRVRVGGSLKIGEQFATGVMTWDTILDYAVYLV
                     KFVGQLALLVGALMIIYYGYKKATEHLKFNSTIGKVVTGILVISFAYVIVRIIRSMFI
                     S"
     gene            complement(1491..2381)
                     /locus_tag="PK674_01165"
     CDS             complement(1491..2381)
                     /locus_tag="PK674_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15175.1"
                     /translation="MMKKIVIIFLIMFAFVGISNANDCNAVLNGTDGIPMITKKRNFD
                     NILPKKAFEKAVENLQIFCCKINDEGKYNYCNNINQTTGVPSSLYLLDHILDVSLRRL
                     DAKEKNKNGEDLIYGLEPDPIGKERRDFITEQGNLTKGSIPLNIVNQYKKYWTVNNDS
                     ILPRLPASSNIPRSEKTFENYQDRTLKERYNGVCESSIFIYINKISGDDKLDYSKLYD
                     AYNKCKSLVDQRIKNEYEYTNIIISQKGGILLHTNINSYLSKYFLQNKILELQQLIFN
                     MKTTFAEINKAVVELTPNCS"
     gene            complement(2476..17175)
                     /locus_tag="PK674_01170"
     CDS             complement(2476..17175)
                     /locus_tag="PK674_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOG15176.1"
                     /translation="MRNRLFKKYSILFVMGIYIVSAFLQSFSFFDFNKVYAQESQLDY
                     TNLVAIFVDDKIYGSLETNIERYAKTYIQGANNNYRYNSISNSKAIVLPVNTDNITAL
                     EITKILENIYFDGISGEPSKLVGVVLIGDIPLPVVNQNGFIYPTIYPYVDFEKQKFIW
                     DDNIKYFVYNNKSKGQAEIRHGLINFDTIDQYRAYFNKLKDYAQNPGEFIDKSIWYED
                     MIANKKYFFADGLGSYINNFLFAEDIGQKRYSDLMVKIMQGTYNDVVVDITNGMGEVG
                     GDISNAIQSLNDSMNTPTKFIDAIVKEGYLRPYTSLMGIKHLDRIVKNVETANRRIEQ
                     ITGSDGKTISRTALDTSYSTIEQKDETLLRLKGGIDPLIVLFNNALEQIVDDKIETEK
                     YRLNEIIPLTYLNYEGARKGKTKLSSCTRKVYDAYENHFFGMPAKYMDNMKQISTYRG
                     TYRNFDNIFGLKIADIQSGAIPSLDLTTIDLNKKSIGGSYDIFATQVDANRGYNINNT
                     TKELEIYDENKIAKRKYRNATCPSKWLGICRKKRKWQSSKEDGEICDIKDKNKQGGCE
                     TLQEFAIRNRGGASPLNLSGTNEWLSGYNFQDAILPIFDIAGSTIVLQPKNTANSFLG
                     VNTYSRLIQQTFVLSQKPKYYFKNKAKEAPSLFGLGYNPNMGEDMKFTNYLPTKDLEE
                     SNVGWEYTEPIKAPSVDFFTWFNNSNYKAWEGDIFKLKKTTDGDCLGKGEILTYMTLD
                     SRVKNVSATNLELNGIIYKIFEDNTSPSKRFYERLSDKITFFYDSINNTIDMSNSTGS
                     ESAMSNLISLKEYISGINRGVANITNTDIKSNMSTGAISDLVNQWDVYVPENMYTNTE
                     NLVENIQIKLVDSYMLLVSFGFEDIEKYIENQISTFTLNKNILVFLYDWKSNLLQTIL
                     NTRNKFLEMHPFVINLENIYNSINDNNLNKNSLLSQLNAKKTKINNIGDGCKDGSKFK
                     PLCNTLDSLKLRVLNQASPVNDEIRKIKKFMVEDDDGNEIEFMPFVVMKLLTSGNEAN
                     INPVLQKLDQIPHSTNVNLTGYIPGMSNITSDRPIDSPKYITFKGVGGDKVTFIYPNL
                     YKAEVFSGNLNALKLKNPKEIETAIKQYLVETVKKYNEYLTIQNTKKTNFYQQNAGAY
                     NALKIKDTLASPIGNRPYNLMPNDYLIVELEKKLEDNIFFKGKMGGYEPIEFIAHLIY
                     YQNIGRQQRKIGEIIGDDINNIIEGFDVNEKIKYSLENYLIKDNNKGTYITPSYRDNG
                     YEVAFINSNGEDLITEKAVPPFAHNIKNSANNFSQPSLPEIDNSSEFLESLNNECGMM
                     DGGGVLIFDINGGVPYSPWLNSLKCRWEKTIALFKDGKKIINVEWPISLKNILNYGVS
                     LQNVGQDMYHNFGVEEMLQYAEQRLYLNNEESNQNVLNNASAIDYQKLDKILSYTVIE
                     TNKPNINADDANGNINIYSTVELNDIDFYIKNIGASKIKIKDGTTNISNNITEGTEGF
                     FTGNFVVDPFYTKELDFEIQDPIAGRNVVVFYMCLPGTQDIAQCVTKTLNLEIIPGKI
                     DKVEILTQGENALQGSKFPITVKGIDKFGNNVGQIFDGKFQISTSSGTLFYESSAGKQ
                     ITFSNFNKSKFVLNIDENATHNSQINIEVQGSIAGADLIEASKNIIVKKGEIKVYKGN
                     SALTNMVINLPSTNEYSYKDSFDLTQMNLNTVPNIKIKLQDIEGQDIDGETLVKISSK
                     NGLIKPGEIEIRKVDVTQNNTTFEVTQKRFSQNNNFMITTGEIEVYFMPSFKAGDDII
                     SISISGLPTLNIPVTVNPAPAKIVEINTEQDSISINSDLQANLKISDNRGNMITKNTM
                     LKIGTLGPLAISGTNGAITNINVTNGQLNFVIKSKEQGGYGFVFAQIMSGGVELEGQK
                     PGAKDIIVQDKILPKKNLNIMYLSLFGNDRGNQRGFMSNNNKYVQELINNSEKLITTT
                     TQLISPEKIKMFSTVMSKKIEFIDLDDKKINLELDNGLNFMIQDVGDINVNINNLIFQ
                     EVGVSEENLEYVIKTLVENKYLDKNALIYIPEATDSIIESNELNGNSIFINDDNVFNL
                     DDMQQNINLSIDLSNDKTAGYQSWELKLNNKKIGTFLFVINKINDLTVQVESNNLEYS
                     YGKIWIDGSTNKEGIGFFQNNSNFPKDSLGYKSIQDSDDPTLGIGFTSDFKNITNFGA
                     GQPVGEATLNFSSEFLINIGDPLLKRIDKNENAKLLDTDLNLELDSGFDKGIGETIFS
                     ETNKTILKVINIDFNNDGLEDVIVAFSDGSVKILKNYGGNAPFKDLGDLMILADGIKE
                     IIVGDVDGNGYKDIIIKNNSDVLRTYKNDRGVFDVDGLPVCINTNVKENIISENPQNT
                     SGVNQIFFEDMNNDGVLDIITSDSLGYIKIFYGGQDNGKDNYISTNKVMCDDERYDRQ
                     KNNTNMVYRFGIRINENVKVLDQSLIHWKGLDVAENIEINTADLGIDTNFFSQIDKDK
                     MQETIQAGTTFDTSSAEEQYKGIERFKLANFGIIPYYEDNIDHEGEIPYVEIGCLTGE
                     DPVKIYKKYEDINGDVLENGDKVQVTIVLKAKKNFTGTFIDKISGPWIIPLTGEMDMI
                     ENFWFEPGTISTGKVENELKFHWDMNNFRYMIDNLSMNAGEEIKIHYRVFYDGQTEIQ
                     TIDIKDVDGNDYEKFGGNEGEKLSNRPKDGLRDIKITPADDCNKSLIILFNNNQGNKK
                     TYSPEYVDLAKILARLNAKAQENFENAMSEISNTLTNGGSDGNGIDLESVPGAGSVFN
                     GNAVLDIWADNFSWDKIMSEGGINLDNILNFPAQVIDGLVGDVAKKVDKIMGGACNGF
                     KLSDFGIGDGKNCGLPVPFNQAFLGPGEYHLFGCFKEPMLPLTHTIGKGLPVLTIPGN
                     RPSPVGYLPIPGIFGLPFKGVKDGFLGVPGGPYNSLFRLYLVPTLTAEIGMAMCFGTY
                     NMLEFVLPDPFGNIAGNCLVTAFPLPCKEEGNGDYESQPNVTNLIPETFLDMDPCTNK
                     NIPCYIGTGESNTSFEFVSSSSTSYNMNSAIPDGSYAGGFITIEKTPVTEYGYRAESA
                     NIEVEGIKLFGGADAQNKILGGDGKGCLKEVLNKRMEQQTDYIMNNLTNFKINIILPD
                     FEGIKADASAIVSSGTYRLTNEQKCLNKGMKRNRETNTCEKQQSGNQNEDKSLTGKKA
                     KKLTKRAQDNWISRDQITDLSNSISNPFVALEQMFKDIDLINIQTENITVKVPMITSD
                     DIVAYISMATEWIKQQEKVLDKRFDLFKALVGRCGGIDKENIKNRSDLKKAIKETKGT
                     LEEQLKEKLKSVEEQMKDIQEQIDATTDPEKKKELEKQKKELESQKDSLAQDVETISK
                     QMGQIKNLNKKYNLNDLKNYEIFESCTAGEFFIKPENVDNLGQILPYDIYISYKPGHE
                     QELSMFTKGFKLNQVKEKTISKKIKISIEKDGESMSNDGLCLKSKQFSTPNQCVELFL
                     GGKLDALLNEFFNIQSNTQLLISSIRQNVETLELYKKFPLELYERMHVGERYMSDIGS
                     LLNGFLGTLSIWMRTNATRYAQYVDAIILLLTTLETYQAIINISKEWSEKCSTCTHDE
                     YDQFSCKIGTLCGMANFPILEIPPFKIPSIFLDFSKLNLGIDITLPKFNFVPVSVPLP
                     RLPGLPEPPSVDISLDMEEALLMGMDLIGKLMEDLGLGVSPSIPAIPIIPAPPKLPEI
                     PSFIPTVRLELPVLPPAPKIPKLPNSIHSILDTIETVGRILCMVKKPIGFVKESAIKA
                     KVEQITQRTYEVPYWDNFDKTMEQRKSESKSKIPEWILNAYPFLKADEFQNVQLKGFD
                     IGIESFVDIQFKFDGVYDFINSLVAMANEFSSVPGEWAQQKIDKLDEKSRELEKRMSA
                     CATNPASEKCLGSLYTGELKENKEELDQLKDEIKNIGNNVRNGFGETKKAILLIEENN
                     NNKEILESEVEKTSSNISYIEQKIEDLNGDLAMSTNEGRIRQLNKEIREYEKLLQSEK
                     RKLNKQQDDLENIKNKINLLDERFGPQIEIYEEYLETYGELEEKYQKLKNKFSEELSG
                     ALETINNMIQSGQQVLDYLHFEEVDERNEKIKENLKKMETKQKSLKEQRYEDVGNFYK
                     QINLGDEISYVDYNKNVYESNSEILKNGLSYILTKSNDLSLNKKIEEYLDIINSKKEN
                     TVLEASEKVQNLEKQYYSIVSEFQKNNIDLSNLIKNDYDKFLYSVANNEISLVNNQDI
                     DVTLSSNLFEANTNYLRKVDSLINLYNIKNNKVKTENIDTEKVKSNSIILDDRKANNK
                     VLLSQNGANSNAGINNNGGLPTIDISQYINTIKTPEGSINLANQDYVTNFQDKSILVD
                     LNGDNKKDLILWDNNNVYVKYGDGNRDYDNGTYYKKYYKYNINSYEKLFDDGEEGFIK
                     INNIYLKMYDENWEVKNFEYEGASFDDIKISWLNSQRLGDDVDGYLVKMIHRVDLFND
                     KEIIIINSNKKFFDKKYMLIVPSGINLSGLKIGLEEGIFDIESLLTGVIWDVLYYNES
                     QQSIGLDITEIPRNWQYSQIYSLNMKENLYYISSPSSNQIVGGPQIVSDETGPKVDIN
                     IFRPSIQEHIDEGSSFDLYVSTNYTIKSDRIDEGGIKKLWIADQDGNIIKEKDNINSI
                     TGYLELDNLYFTGVGVKNYHFVGMDINGNTSSTEITLNIKKPDIEITDIVKYGNENFG
                     ISSPVTISAELSHDVDEAYVQFLRNRNGIREILTGTMGGIEIDKYKLSPLQMIITGGY
                     YDFGNDIGLYISSGSLVAKLNPKNGKISIENGFENIIKVNLDYSKKIPIIKVLDDKNI
                     TIFSIKLTSKELVSIDTNLEMRELHGDNFGDFNNGKAIIYNNEVLLYVSPTGKLYTDE
                     LIYGDYGFDDDSESVVYSFRTNNWGQNLGTIKIKIQDLLSE"
BASE COUNT         5479 a         2839 c         2049 g         7110 t
ORIGIN      
        1 tctcttccct ttcatttagc ataaaaatat ataatataat aaaacatttt atcataatat
       61 aatgttgttt atgacaaaac atcccaatct aaaagacttt ttgccaaaaa ataaggattc
      121 gaaaccttta ccttttatag atcatctttt atcaaaactg gatgaacata tttccaaaaa
      181 ttgaaaaatt ttgattttaa ctcttaccaa aaagtctagt gaggaaatta caaactatct
      241 cgtttccaaa tgatacaaag cctactattt gcacagtgaa atagtaacta tggatcgttg
      301 ggagattcta aaaaagctca aaacttgaga aatagatatt atagtatgag tcaatttatt
      361 gagagaatga atagacttgc ccgaggtttc ttttcttgct atattagatg ctgacaaaga
      421 atgatttctt cgctcttcga cttctcttat ccaaatcatt ggtcgtgcct ctagaaatcc
      481 aaactctgag gttgtccttt atgctgatca attcactgaa tctatgatta aatctctgcg
      541 agaaacttat cgtcgtagaa aaattcaaga tgagtttaat aaaaaatata atattactcc
      601 caaaaaagct acatcaaata tcaaagatct agaatcagta aggacggatc aagacttagg
      661 tcaacatttt tctgctatta caaaatgaaa ggttaaaaga ctcaaaagaa tgacaaagaa
      721 agaaaaagaa tttatctcca aaaatctcaa gaaacaaatg gatttggcaa tcaaagaatg
      781 gaggtttgag gatgctaccg ttattcgtga tcagatcaaa gagctagagg aggggattgt
      841 ttagatacat aatttaacaa aaaagagttt atacataaat tttatatgaa aaattaatgg
      901 aataggttca tagaaataaa aaacagtaga tattgttatt gttttttatc acataaatat
      961 tttaatatca aaaatttatt aagaaataaa catacttcga attattctca ctataacata
     1021 ggcaaaactt attaccaata ttcatgtgac aacctttcat atagttgaat taaatttaag
     1081 atgttctgta gcttttttgt atccatagta aattatcata agggctcaca ccagcaaagc
     1141 caattgtcag acaaatttta caagatatac agcatagtcc aaaatagtat cccaagtcat
     1201 taccccagtc gcaaactgtt ctcctatctt taaacttcat catacacgaa ctctttttca
     1261 atctactata ttctctggtc aatatgcagt atctttgtat tttttccata cacttcatca
     1321 ctctctaata ttatcaacaa cagctcctac ggcagatgca gatgattccg tttctggtat
     1381 tacttcaaat caaacttgag caatagcaat agtgctaaat ccagctatta taactgacaa
     1441 tagtgcaata ttttttttta tcattttaaa tttgttatat tataaattta ttagctacaa
     1501 tttggtgtta gttctacaac agctttattt atttcagcaa aagtagtttt catattaaat
     1561 atcaattgtt gtaattctag tatcttattt tgtaaaaagt atttgcttaa ataagaatta
     1621 atatttgtat gtaataatat tcatcacttt tgtgatataa ttatattagt atattcgtat
     1681 tcgtttttaa ttctttgatc aactaaagat ttgcacttgt tataagcatc ataaagtttg
     1741 ctgtaatcta atttgtcatc gccacttatt ttatttatat agataaatat actactttcg
     1801 catactcaat tatatctttc ctttaaagtt cggtcttgat aattttcaaa tgttttttca
     1861 cttcgtggta tattagaact agctggtaat ctaggtaata tactatcgtt atttacagtc
     1921 cagtattttt tatattgatt tacaatattc aaaggtatac ttcatttagt tagatttcct
     1981 tgttccgtta taaaatctct tcgttcctta ccaataggat caggttccaa tccataaatt
     2041 aaatcttctc catttttatt tttttccttg gcatcaagcc ttcttagtga aacatctaat
     2101 atgtgatcaa gtaaatataa agatgatgga actcctgtag tttgatttat attattgcaa
     2161 taattgtatt ttcattcatc attaatttta cagcaaaaaa tctgcaaatt ttccacggct
     2221 ttttcaaaag cttttttggg caaaatattg tcaaaatttc gtttttttgt tatcattgga
     2281 attcaatcgg taccattaag tactgcatta cagtcgtttg cattacttat tccaacgaaa
     2341 gcaaacataa tcaagaatat aataactatt tttttcatca ttatttttaa aattaaaact
     2401 ttatatctac tattattata atatattttt tgttttttgc aaatttttca ccaaatcaaa
     2461 taattcattt ttttattatt cggataataa atcttgtatt tttattttta ttgttcacaa
     2521 attctgcccc caattattgg tcctaaaaga atatactaca ctttcgctat cgtcgtcaaa
     2581 tcaataatct cagtagatta gttcatctgt atatagcttt caagtcggac tcacatacaa
     2641 taatacttca ttattgtata ttatagcttt accattatta aaatcgccaa aattatctcc
     2701 gtgtagttct ctcatttcca aatttgtgtc aatacttacc aactctttgc tggtcaattt
     2761 tatactaaat atagttatat ttttgtcgtc caaaaccttt atgataggga ttttttttga
     2821 gtaatctaaa ttcactttaa ttatattctc aaatcaattc tctatactta tttttcaatt
     2881 tttaggattt agttttgcaa ccaaacttca gctcgatata tataatcata tgtcattgcc
     2941 aaaatcataa tatcacccag taattatcat ttgtaacgga cttagcttat atttatcgat
     3001 ctctattcca cccattgttc atgttaatat ttcccggatt ccgtttctgt ttctcaaaaa
     3061 ttggacataa gcttcatcta catcgtggct taattctgca cttattgtta ctggagaact
     3121 tattccaaaa ttttcatttc aatattttac tatgtccgtg atttcaatat caggtttttt
     3181 tatatttaat gttatttcag ttgaacttgt attgccgttt atatccattc aaacaaaatg
     3241 atagtttttt acacccactc atgtaaaata taggttatcc aactctaaat atcatgttat
     3301 gctgtttata ttatcttttt cttttataat attcccatct tgatcagcta tccataattt
     3361 ttttattcaa ccttcatcta ttcgatcaga tttaattgta taatttgtgc tcacatataa
     3421 atcgaaacta ctaccttcat ctatatgttc ttgaattgag ggtctaaata tatttatatc
     3481 aaccttaggt catgtttcat cagacactat ttgtggtcat catacaattt gattactaga
     3541 aggactagaa atataatata aattctcttt catgttcaag ctatatattt ggctatactg
     3601 ccaatttctt ggtatttctg taatatccag tcatattgat tgttggcttt cattataata
     3661 taatacatcc caaattaccc cagttaaaag agattctata tcaaaaattc actcctccaa
     3721 tcatattttt aatcagctta gatttattca gcttggaaca attagcatat attttttatc
     3781 aaaaaacttt ttattgctat ttattattat aatctcttta tcattaaata aatctactct
     3841 atgtatcatt ttaactaaat atccatctac atcatctcac aatctttggc tattcaacca
     3901 acttattttt atatcatcaa aacttgctcc ttcatattca aaatttttga cttcccaatt
     3961 ctcatcatac attttgagat aaatattatt tatttttata aatcattctt ctccgtcatc
     4021 aaataacttt tcataggaat ttatgttgta tttatagtat tttttataat atgttccatt
     4081 atcgtaatct ctatttcaat ccccatattt aacatagaca ttattgttat cccaaaggat
     4141 taaatctttt ttattatctc agttcaagtc tactaatata cttttatctt ggaagtttgt
     4201 tacataatct tgatttgcta aatttatgct tcattcagga gttttgattg tatttatata
     4261 ttgagaaata tctatagtag gaagtcatcc gttattattt attcaagcat tactatttgc
     4321 accgttttga ctcaataaaa ctttattatt tgctttccgg tcatctaata taatagaatt
     4381 tgattttact ttttcagtgt caatattttc tgtttttact ttattgtttt taatattata
     4441 taaatttatt aaactgtcta cttttctcaa ataattagta tttgcttcaa ataaattgct
     4501 agatagtgtt acatcaatat cttgattatt taccaaagat atctcattat tcgctacgga
     4561 atataaaaac ttatcataat cgttttttat taaattactc aaatcaatat tgtttttttg
     4621 gaattcactt acaatactat aatattgttt ttctaggttc tgcacctttt cacttgcttc
     4681 caatacagta ttttcttttt tactatttat tatatctaga tattcctcaa tttttttatt
     4741 taaagataga tcatttgatt ttgttaaaat ataacttaat caatttttta gaatttccga
     4801 attggactcg taaacattct tattataatc aacataagaa atttcatctc atagatttat
     4861 ttgtttatag aaatttcaaa catcttcata tctttgctct tttagagatt tctgttttgt
     4921 ctccatcttt tttaaatttt cttttatttt ttcatttcgt tcatccactt cttcaaaatg
     4981 cagatagtct aatacttgct gtcaactttg aatcatgtta tttatagttt ctaatgctcc
     5041 agacaattct tcagagaatt tatttttaag tttttggtac ttttcttcta attcaccata
     5101 agtttctaaa tactcttcat atatttctat ttgaggtcaa aatctttcat ccaacaaatt
     5161 aattttattt ttaatgtttt ctagatcatc ttgttgctta tttaatttcc ttttctctga
     5221 ttgcaatagt ttttcatatt ctctaatttc tttatttaac tgtctgattc taccttcgtt
     5281 tgtactcatc gctagatctc aattcaaatc ttcaattttt tgttctatat atgaaatatt
     5341 acttgatgtt ttttctactt cagattctaa aatctcttta ttattattat tttcttctat
     5401 caataaaata gctttctttg tttctccaaa accatttcta acattatttc aaatattttt
     5461 aatctcatct tttaattgat ccaattcttc tttattttct tttaattctc atgtatacaa
     5521 acttcacaaa cacttttcag aagcaggatt tgtcgcacaa gcactcattc ttttttctag
     5581 ttctcttgat ttctcatcca atttatcgat tttttgttgt gcccactctc aaggaacaga
     5641 gctaaattca ttagccattg cgaccaaaga atttatgaaa tcatacaccc catcaaattt
     5701 aaattgaata tccacaaaag attctattca tatatcaaat catttaagtt gaacattttg
     5761 gaattcatct gccttcaaaa acggatatgc atttaaaatc cattctggta ttttactttt
     5821 ggattcactc tttcgttgtt ccatagtttt atcaaaatta tcccaatatg gtacttcata
     5881 agttctttga gtaatttgtt ctacttttgc tttgatagca ctttccttta caaatcaaat
     5941 aggtttttta accatacaca aaattcttcc tactgtctct atagtatcca gtatagaatg
     6001 tatactgttt ggaagttttg gtattttggg tgctggtggt aatactggaa gttccaatct
     6061 tacagtgggt ataaaagatg gtatttccgg taattttggt ggtgcaggaa ttattggaat
     6121 tgcagggatg gatggagaca cccccaagcc aagatcttcc attagtttgc caattaaatc
     6181 cattcccatt agcaaggctt cctccatatc tagtgatata tcaacacttg gtggttctgg
     6241 taagcctggt aatcttggta aaggtacaga aactggtaca aaattaaatt ttggcagagt
     6301 tatatctatt cacaagttta acttagaaaa atctagaaat atgcttggta ttttaaatgg
     6361 tggaatttct aggataggga aattagccat accacacaaa gttcatattt tacaactaaa
     6421 ctgatcatat tcatcatggg tacatgtact acatttttcc gaccattctt ttgaaatatt
     6481 tattattgct tgataggttt ctagtgttgt taataataaa attatcgcat ccacatattg
     6541 ggcatatcta gttgcatttg tacgcatcca tatagataat gttcataaga acccatttaa
     6601 taaagatcaa atatctgaca tatatctttc acccacatgc attcgttcat ataattcaag
     6661 agggaatttt ttatataatt ctagagtttc aacattttgt cttatgctcg aaataagtaa
     6721 ttgagtattg ctttgtatat tgaaaaattc atttaataaa gcatctaatt tccctcataa
     6781 aaataattcc acacattgat ttggagtaga aaattgttta ctcttcaagc aaagtcagtc
     6841 gttgctcata ctttctcagt ctttttctat tgaaattttg attttctttg aaatagtttt
     6901 ttctttaact tggttcaatt taaatcactt ggtaaacata gacaactctt gctcatgacc
     6961 aggtttgtaa gatatatata tatcatatgg aagtatctgc cccaaattat ctacattttc
     7021 aggttttata aaaaattctc cagccgtaca agattcaaaa atttcataat tttttagatc
     7081 atttagatta tattttttat tcaaattttt aatttgtccc atttgttttg atatagtttc
     7141 cacatcctgt gccaatgaat ctttttgtga ctctagctct tttttttgtt tttctaattc
     7201 tttcttcttt tctggatcag tagttgcatc tatttgttcc tgaatatcct tcatttgctc
     7261 ttcaacagat tttaatttct ctttcaattg ttcttccaga gttccttttg tttcttttat
     7321 agcttttttt agatcagatc gattttttat gttctccttg tctattcatc cacatcgccc
     7381 aactagcgct ttaaatagat caaatcgttt atctagcacc ttttcttgtt gttttatcca
     7441 ttctgttgcc atactaatat atgcaacgat atcatcagaa gttatcattg gcacctttac
     7501 tgtaatattt tccgtttgta tgtttatcaa atcaatatct ttgaacattt gttctagtgc
     7561 aacaaaaggg tttgatatag agttagatag atctgttatt tgatctctac taatccaatt
     7621 gtcttgagct cgtttagtta gttttttagc tttctttcca gtcagacttt tatcctcatt
     7681 ttgattccca gattgttgtt tttcacaagt attcgtttct ctatttcgtt tcattcattt
     7741 atttaaacat ttttgctcat ttgttagtct atatgttccg gatgaaacaa tagctgaagc
     7801 atccgctttt attcattcaa aatcaggtaa aataatattt attttgaaat ttgttaaatt
     7861 attcattata taatctgtct gttgttccat tcgtttgtta agcacctctt taagacatca
     7921 ttttcaatct catcataata ttttattttg tgcatcagct catcaaaaca gtttaattcc
     7981 ctcaacttct atattagctg actctgctct atatcagtat tcagtaacag gagttttttc
     8041 aatagtaata aatcatcaag cataacttcc atcaggaata gctgaattca tattataact
     8101 agtgctacta ctagacacaa attcaaaact tgtatttgat tctcatgtcc ctatatagca
     8161 tggtatattc ttattagtac aagggtccat atccagaaat gtttctggaa taagattggt
     8221 tacatttggt tgagattcat aatctcaatt tcattcttct ttacaaggta aagggaatgc
     8281 cgtcactaaa caatttcagg caatatttcc aaaggggtct ggtaacacaa attccagcat
     8341 attatatgtt ccaaaacaca tagccatccc aatttcagca gttagtgttg gtaccaaata
     8401 tagtctaaac aaagaattat atgggcctca cggaactcat aaaaatcaat cttttacacc
     8461 tttaaacggc aatcaaaata ttcatggtat tggtaagtat ccaactggtg atggtcgatt
     8521 tccaggtatc gttaatactg gaagtccctt tccaattgta tgtgttaatg gtaacatagg
     8581 ttctttaaag catccaaata agtgatattc tccaggtcat aaaaacgctt gattaaatgg
     8641 gacaggtaat ccacagttct tcccatcacc aattccaaaa tcactaagtt taaacccatt
     8701 acaagctcat cccataattt tatctacctt ttttgcgaca tctcaaacta atcaatcaat
     8761 tacttgtgca gggaaattaa gtatattatc taaattaatt cctcattctg acataatttt
     8821 atcccagcta aaattgtctg cccaaatatc aagaacagcg tttcaattaa aaacacttcc
     8881 tgctccaggg acactttcca aatctatacc atttcagtca gaaccgccat tagtgagtgt
     8941 atttgagatt tcactcatcg cattttcaaa attctcttgt gcttttgcat ttaatctcgc
     9001 caatattttt gccaaatcta catattccgg agaataagtt tttttatttc actgattatt
     9061 attaaacaat ataatcaagg atttattaca atcatcagct ggagttattt taatatctct
     9121 taatcaatct ttcggacgat tacttaattt ttctcactca tttcatcaga atttttcata
     9181 atcatttcaa tctacatcct ttatgtctat tgtttgaatt tctgtttgcc catcataaaa
     9241 cactcgataa tgtattttta tttcctctcc agcattcata gataggttgt ctatcatata
     9301 tctaaaatta ttcatatccc agtgaaactt caattcattt tcaacttttc atgtagaaat
     9361 tgttcctggt tcaaaccaaa aattttcaat catatccatt tctccagtca atggaataat
     9421 ccaaggtcaa gaaattttat ctataaatgt tcaagtaaaa tttttctttg ctttcagtac
     9481 tattgttact tgcactttat ctcaattttc taaaacatct cagtttatat cttcatattt
     9541 tttgtatatt tttacagggt cttctcaggt taaacatcat atttccacat aaggtatctc
     9601 tcattcatga tctatattat cttcataata aggtattatt ccaaagtttg ctaacttaaa
     9661 tctttctatt catttatact gttcttcagc gctacttgta tcaaatgtag ttcatgcctg
     9721 aatagtttcc tgcattttat ctttatctat ttgtgaaaag aaattagtat ctattcataa
     9781 gtctgctgtg tttatttcaa tattttctgc aacatctagt catttccaat ggatcaagct
     9841 ttggtccaaa acttttacat tttcattaat tcttattcca aatctataca ccatgtttgt
     9901 attgtttttc tgtctatcat atcgttcatc atcacacatg actttattgg tagaaatata
     9961 gttatctttt cagttatctt gtccaccata aaaaatctta atataaccca aactatcgct
    10021 tgtaataata tccaaaactc catcattatt catatcttca aaaaatattt gatttactca
    10081 tgatgtgttt tgaggatttt ctgatataat attttctttt acatttgtat ttatacaaac
    10141 aggcaatcaa tccacatcaa atactcagcg atcattttta taggttctca aaacatcgga
    10201 attatttttt attattatat ctttatatcc gttcccatct acatccccca caataatttc
    10261 ttttattcag tcagctaaaa tcattaaatc ccccaaatct ttgaaaggag catttcatca
    10321 atagtttttt aatattttta cacttccatc cgaaaatgct acaataacat cctccaagcc
    10381 atcgttatta aaatctatgt ttatcacttt caatattgtt ttatttgttt cagaaaagat
    10441 tgtttctcat attcctttat caaagccact gtctaattcc aaatttaaat cagtatctaa
    10501 taattttgca ttttcatttt tatctattct ttttaataat gggtctccta tatttattag
    10561 aaactcagaa gaaaaattta gagttgcttc tcaaacaggt tgaccagctc caaagtttgt
    10621 tatatttttg aagtcacttg taaatcatat tcataaagta ggatcatcac tatcttgtat
    10681 agatttatat cccaatgaat cttttggaaa atttgagttg ttttgaaaaa atccgattca
    10741 ctctttattg gtagatcaat caatccaaat tttaccataa ctatattcta aattatttga
    10801 ttctacttgc acagtgagat cgtttatttt atttattaca aacaaaaagg ttcatatttt
    10861 tttattgttc aattttagtt cccaagattg atatcaagca gttttatcat tactcaaatc
    10921 tatacttaga tttatgtttt gttgcatatc atccagatta aacacattgt catcgtttat
    10981 aaatattgaa ttcccattaa gctcattgct ttcaataata ctatccgttg cctctgggat
    11041 ataaattagt gcatttttat ccaaatattt gttttccacc aaagttttta ttacatactc
    11101 cagattttct tcgctaacac caacttcttg aaaaattaaa ttatttatat ttacattaat
    11161 atcgccaaca tcctgtatca taaaatttaa tccgttatcc aattcaaggt ttattttttt
    11221 atcatctaga tctataaact ctattttttt agacataaca gtagaaaaca tttttatctt
    11281 ttctggactt ataagttgtg tggtagttgt tattagtttt tcagaattat taattaattc
    11341 ttggacatat ttgttattat tagacataaa acctcgttgg tttcatcgat cgttcccaaa
    11401 taaactcaaa tacattatat ttaaattttt tttgggtagg attttatctt gtactatgat
    11461 gtcttttgct catggttttt gtcattctag ctcaactcat caactcataa tttgtgcgaa
    11521 tacaaatccg tatcatcatt gttctttcga ctttattaca aaattaagct gtcagtttgt
    11581 aacatttata tttgtaatag ctccgtttgt tcaagaaata gcgagtggtc atagagtacc
    11641 aatttttagc attgtatttt ttgttatcat atttcatcga ttatcagaaa tttttaagtt
    11701 tgcctgtaaa tctgaattta tagagataga atcttgttct gtatttattt ccacaatttt
    11761 tgcaggtgca gggtttaccg ttactggtat gtttagagtt ggtaatccag agattgatat
    11821 tgaaattata tcatctcatg ctttaaagct tggcataaaa taaacttcta tctcaccagt
    11881 tgttatcatg aaattattat tttgagaaaa tcttttttgt gtaacttcaa atgtggtatt
    11941 attttgtgtc acatctactt ttcttatttc tatttcacct ggttttatca gcccattttt
    12001 gctagagatt tttacgagag tttcaccatc tatatcttgt cattcgatat cttgtaattt
    12061 tatttttata ttagggaccg tatttaggtt catctgcgtt aaatcaaatg aatctttata
    12121 ggaatattcg tttgtagaag gtagatttat taccatgttt gttaaagctg agtttcattt
    12181 atagacctta atttctcatt tttttactat gatattctta gatgcttcta tcaaatctgc
    12241 tccagcaata cttcattgta cctctatatt tatttggcta ttgtgagtag cattttcatc
    12301 aatattcaac acaaatttag atttgttgaa gttagaaaat gttatttgtt ttcatgcact
    12361 agattcgtag aaaagggttc cacttgatgt agatatttgg aatttaccat caaatatttg
    12421 tcatacatta tttcaaaatt tatctattca cttgacagtt atagggaatt tcgatcactg
    12481 taatgcattt tctcattgtg tcaaaatttc tactttatca atttttccag gtatgatttc
    12541 taggtttaga gtttttgtta cacattgtgc tatatcttga gttccaggta aacacatata
    12601 aaacaccaca acatttcttc aagcaatagg atcttgtatt tcaaagtcca actcttttgt
    12661 ataaaaagga tctacaacaa aatttcctgt aaaaaatcat tctgttcatt cagtaatatt
    12721 atttgagata tttgttgttc aatcttttat ttttatttta cttgctccaa tatttttaat
    12781 ataaaaatct atatcattta gttctactgt ggaatatata tttatatttc agtttgcatc
    12841 atccgcattt atattaggtt tgtttgtttc aattaccgta taagataaaa ttttgtctaa
    12901 tttttgataa tctatagcac tagcattgtt taatacattt tggttacttt cttcattgtt
    12961 caaatataat cgttgttccg catattgaag catttcttct acaccaaaat tatgatacat
    13021 atcttgtcaa acattctgta gagaaactca ataattcaag atgtttttca aagaaatcgg
    13081 ccattctaca tttataattt tttttcaatc tttaaaaagt gctatagttt tttcccatcg
    13141 acattttagt gaattcaacc acggagaata tggcaccccc ccatttatat caaatatcaa
    13201 cactcagcca ccatccatca ttcagcactc attgtttaaa ctttccaaaa attcactaga
    13261 attatctatc tctgggagac taggttgtga aaaattatta gcagaatttt ttatattatg
    13321 agcaaaagga ggtactgctt tttctgtaat taaatcctct ccattagaat taatgaaagc
    13381 aacttcatat ccattgtcac gataagatgg ggtaatataa gttcatttgt tattatcttt
    13441 tattaaataa ttttccaaac tatattttat tttttcattt acatcaaaac cttctataat
    13501 attgtttatg tcatctcata taatttctca aatctttctt tgttgtcgtc caatattttg
    13561 ataataaatt aggtgagcta taaattctat tggttcatat catcacattt ttcatttaaa
    13621 aaagatatta tcctccagtt ttttttctaa ctcaacaatt aaataatcgt ttggcatcaa
    13681 attatatgga cggtttcaaa tgggtgatgc tagtgtatct tttattttta atgcattata
    13741 agctcaggca ttttgctgat aaaaattagt ttttttggtg ttttgaattg ttagatattc
    13801 attatatttt tttacggttt ctaccaaata ttgtttgata gcagtttcta tttcttttgg
    13861 atttttcaat ttcaatgcat taagatttca actgaaaact tctgctttat aaagatttgg
    13921 ataaataaat gtaactttat ctcatcatac tcatttaaat gtaatatatt ttggagaatc
    13981 tataggtcta tcggaagtga tgttactcat tcaaggtata tatcaagtta gattgacatt
    14041 ggtagaatgg ggaatttgat ccagtttttg tagaacaggg tttatgttag cttcatttcc
    14101 tgaggtcaga agtttcatta caacaaatgg cataaattct atttcattac catcatcatc
    14161 ttcaaccata aattttttaa tttttctaat ttcatcatta actgggctag cttgattgag
    14221 aaccctcaac ttcaaactat ctaaagtgtt acataaaggt ttaaatttac taccatcttt
    14281 acatccatct cctatgttat ttattttagt ttttttagca tttaattgac ttagtaaact
    14341 attcttattt aaattattat catttatact attataaata ttttccaaat ttattacaaa
    14401 aggatgcatt tccaaaaatt tatttcttgt atttaaaata gtttgtagta aattactttt
    14461 ccaatcatac aaaaacacca gaatattttt atttaatgta aatgtagaaa tttggttttc
    14521 tatatatttt tcaatatctt caaatcaaaa actcacaaga agcatgtatg agtcaacgag
    14581 ttttatttgt atattttcaa ctaaattctc tgtattagta tacatatttt ctggaacata
    14641 aacatcccac tgatttacta aatcacttat ggctcaagta ctcatgtttg attttatatc
    14701 tgtattcgtt atgttagcta ctcctctatt tattcaagaa atatattctt ttaagctaat
    14761 caaattagac atcgcagatt ctgaaccagt agaattggac atgtctattg tattattaat
    14821 tgagtcataa aaaaatgtta tcttatcaga taaacgctca taaaatcttt tagatgggga
    14881 ggtgttgtct tcaaagattt tataaattat tccatttaat tctaaattgg tagcagaaac
    14941 attctttact ctggagtcta aagtcatgta tgttaatatt tcacccttac ccaaacaatc
    15001 tccatcagta gtttttttta atttaaatat atctcattcc caagctttat agttactgtt
    15061 attgaaccaa gtaaaaaaat ctacactagg tgctttgata ggttctgtat attcccatcc
    15121 cacattagat tcttctaaat cttttgttgg taaatagttg gtaaatttca tatcttctca
    15181 catattaggg ttgtatcata gcccaaataa actaggagct tcttttgctt tatttttaaa
    15241 ataatattta ggtttttggc ttagtacaaa agtttgttgt atcagtctag agtaggtatt
    15301 tactcataaa aagctattag ctgtattttt aggttgaaga actatagtgc ttcatgctat
    15361 atcaaagatt ggtaaaatag catcttggaa attatagcca gataaccatt cattagttcc
    15421 agatagattt aatggagatg ctcctcatcg atttctaata gcaaattctt gtaatgtttc
    15481 acatcatccc tgtttgttct tatcttttat gtcacaaatt tctcagtctt ccttactaga
    15541 ttgccatttt cgttttttcc gacatatccc aagccacttg cttggacaag tagcatttcg
    15601 atattttctt ttagctattt tgttttcatc atatatctcc aattcttttg ttgtattatt
    15661 tatattatat cctctatttg catctacttg agtagcaaat atatcgtaag atcctcatat
    15721 agactttttg ttcaaatcta ttgttgttaa atccaaagaa gggattgctc aagattgaat
    15781 atcagctatt tttaatcaaa atatattatc aaaatttcta taagttcatc tgtaagtaga
    15841 tatttgtttc atgttatcca tatatttggc tggcatacca aaaaagtggt tttcataagc
    15901 atcatagact ttccgtgtac aacttgataa ttttgttttt catttacgag ctcattcata
    15961 atttagataa gttaaaggga taatttcatt caatcgatat ttttcggttt ctattttatc
    16021 gtccactatt tgttctaagg cattattaaa taacacaatt aaaggatcta ttcatcattt
    16081 taatctcaat aaagtttcat ctttctgttc tatcgtagaa taagaagtat ccagtgctgt
    16141 acggctaatt gtttttccat cagatcaagt aatttgttct attcgcctat ttgcagtttc
    16201 tacatttttt acaattctat ccaaatgttt tattcacatc aaagatgtat aaggtctgag
    16261 gtatcattct ttgactattg catctataaa tttagtcgga gtattcatgc tatcatttaa
    16321 agactgaata gcatttgata tatctcatca tacttctccc attccatttg ttatatcaac
    16381 tacaacatca ttatatgttc attgcattat ttttaccatc aaatctgaat atcttttttg
    16441 tccaatatct tcagcaaata aaaaattatt aatataagac cccaatccat cagcaaaaaa
    16501 gtacttttta tttgctatca tgtcctcata ccaaatgctt ttatcaataa attctcaagg
    16561 attttgtgca tagtctttta gtttattaaa ataagctctg tattgatcaa ttgtgtcaaa
    16621 atttattagc ccatgccgaa tttctgcttg tcattttgat ttgttgttat acacaaaata
    16681 ttttatatta tcatcccaaa taaatttctg tttttcaaaa tcaacataag gataaatggt
    16741 gggataaata aatcaatttt gatttaccac aggtagaggt atatctcata ttagaactac
    16801 tcaaacaagt ttagatggtt ctccactaat tcaatcaaaa tatatgtttt ccaaaatttt
    16861 tgtaatttct aaggctgtga tattgtccgt atttaccggc aaaactatag ctttagagtt
    16921 tgatatagag ttgtatctat agttattatt tgctcattgt atataagttt tcgcatatcg
    16981 ttcaatattt gtttccaagc ttcaatatat tttatcatct acaaaaatag caactaaatt
    17041 agtataatct agttgacttt cttgagcata cactttatta aaatcaaaaa aagaaaacga
    17101 ttgtaaaaat gcagatacga tgtatattca cattacaaac aatattgaat attttttaaa
    17161 tagtctattt ctcatatttg tattttatat ttttataaat gtttttttca ttttccgcaa
    17221 tcaattgtac atataaaaat tttttagtaa aaaaatctga caaaatatca cacaattgac
    17281 tttgtattca taaaaaataa caagtaatat atttacatat atttatttat atcaaattta
    17341 tataagatta tttaaaatga tcacaaaatt gagagttgac ttttatttat aaacattcac
    17401 aaccaattat ctaaaattac agcagtactg tccgggaaaa ttttagatat aagaaaataa
    17461 tgaaaattga ggaggcg
//
LOCUS       DAOSID010000010        17225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_3518_length_17225_cov_15.075364, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000010 DAOSID010000000
VERSION     DAOSID010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 17225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17225
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3518_length_17225_cov_15.075364"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1271)
                     /locus_tag="PK674_01175"
     CDS             complement(78..1271)
                     /locus_tag="PK674_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOG15177.1"
                     /translation="MKKQDFPIFQNNPTLVFLDSAASTQKPQYVIDGVNDFASNDYAN
                     IHRGAYQLSEKSEELYDKSKQLLAEFLNSKASEIIYNYNATHGINVIAQSLVNSGFLK
                     KGDKILLGIREHHANIVPRQILSKIFGFQIDFVGVSKGCEIDRDDFSKKYDKSVKVVS
                     IGHVSNVTGKIYDVKKIKSLLRADTFFIIDGSQSFPNMKIDVQDIGCDCFVFTAHKVM
                     AYTGIGAMYLKRDWIKKLTPMMSGGGAIRDVDLFGHTVPNDYSKFEAGTPNIIGAISL
                     LKSLEYIKSIGGMEKIREHEQKLVEYSLERFTKLKEKVKLIGPKTNDRTAVFSFFIPG
                     NENFNHIGEIFAQHNIAIRCGGHCAYPMHKDLKIPGTCRMSAYLYNDISDLEKFFEVL
                     ESIVK"
     gene            complement(1324..2166)
                     /locus_tag="PK674_01180"
     CDS             complement(1324..2166)
                     /locus_tag="PK674_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15178.1"
                     /translation="MYTLVKNRPFQKRKTNNIKRKIKKIKLIYPEINFPIKRFWIFLL
                     SIIFIYGIFFIIQHTIFKDENYIKKVYYSQTSVDTYDDPYLYNTISKLIKDENYYVVS
                     KFKRKAILHTLKQDFPIVKNIRIIQPEKFSASVQIIFYDLDMVLQLGNRKFGVLGYYN
                     FEIFSGNTIGDGVFTVELPKYLSGTESINGLFVEISPEKLISDIDVISQGFNGYSRIV
                     YLPGSSMTVVFVGGKTIYLNNKNSLTGQIDNYNLLMKYYNEANSLKIIDLGSLEGDKI
                     IVKK"
     gene            complement(2174..2659)
                     /locus_tag="PK674_01185"
     CDS             complement(2174..2659)
                     /locus_tag="PK674_01185"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="divergent PAP2 family protein"
                     /protein_id="HOG15179.1"
                     /translation="MFSFIRESFKTYFQEGIKINPLLILIFVGFVTQLIKITIDSIKQ
                     KKFCISNFFSSGGFPSFHSSIVSSIAIMVLIEFGFDSIYFALACAFAGLVVYDAMNLR
                     FESGKQAEYINEIRLNITSVLTMTQKESLKERLGHTPFEVFAGIILGIALTFVLYYYF
                     I"
     gene            complement(2877..3257)
                     /locus_tag="PK674_01190"
     CDS             complement(2877..3257)
                     /locus_tag="PK674_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15180.1"
                     /translation="MLLIFDEIDEFKKDFKKLLKKFETLDEDFSIFKQFALSTYHVNG
                     INNGGFFPITGQGVHDYTFCPIKVKKFACKSLKGKGARSGIRIIYNFSKKNNKITFIQ
                     IYFKEKADTDCDYQRIKHFYKLMK"
     gene            complement(3270..4058)
                     /locus_tag="PK674_01195"
     CDS             complement(3270..4058)
                     /locus_tag="PK674_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF4065 domain-containing protein"
                     /protein_id="HOG15181.1"
                     /translation="MILKRLIAQKLADLRKTFGYSQKELSKVLGVDRVVYTNIESGLR
                     EIKVDELKKLADFFQIQIEYFFTNEKNTDHYKIIKDDKNEIRVSIPTCQEEKFKEVFL
                     YILEKLSAKPSFSETVLHKILYFCDFDYYEKYEEFFIGSRYIRNHQGPTSKELIKIIK
                     EMKEKKEIKQENIHFKNGHTGRKYIPLRKADLTKINGAEKEILDNVIQKLGDMGASRI
                     SEYVHGDVPRISTADQAEIPYEAVFYRSPQYSIRDYEENDDFSI"
     gene            4342..5721
                     /locus_tag="PK674_01200"
     CDS             4342..5721
                     /locus_tag="PK674_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG15182.1"
                     /translation="MKKLYKFIAVVLLVNVLFFKTYTSTSANSPKDIQNHKYMESIQY
                     LYDHGVVQGYPDGNFRPDREINRGEIMKIILKSSIGPDLGELNNCFEDVKDERFAKYV
                     CYAKSQNMVKGYDDGKFRPYQYVSFAEALKMGIEGFSKKTESVSNGEPWYQGYLNFVH
                     NNNIFSKYEIFPYKNMTRGEMAYFIHQFMLEKEGKISFGNQRKPGSSGCGKEPPATPI
                     KSSVVNGVVRNYITVIGNRYDKNKPIKLILAFHGRTNSNEMVRSYYGIEKATNGNAII
                     IYPSGLPENSSPRNRSNPGDKKSNLRDFALFDQILKDFEENYCIDKDQIFVVGHSLGA
                     WFTNSLSCARGDKIRAIGSIGGGTTINECSGPVAGIIMQHPDDNLSPYREGVVARDQM
                     LKQNFCGPETVPFGPAPLNCVKYTNCLSDAPVIWCPHSDSTDNRGKYYPHTWPDNAGS
                     EIIKFFESL"
     gene            5747..6553
                     /locus_tag="PK674_01205"
     CDS             5747..6553
                     /locus_tag="PK674_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="CAP domain-containing protein"
                     /protein_id="HOG15183.1"
                     /translation="MKKFLFVFIFGFVFLNFLGFTNAEYPVSEKVKTRIDGIMSIVEK
                     QRGEKSIYRQMDRYQNIVNSFSLLKNNLDGEQKEMIGYLVYLFENKLIKLKEMSINQS
                     DILKNVDRDRVQEEILRWHNEERNKLGLSDYIINDKLNYSALVWADNLAKNNRKSSTH
                     TRTPGDGYYNYDKILSWFNNLGIDFDYKRTAFTENVAYQVYSCNKSDCTQDMINALKK
                     GFDFWMSEKGRSYRPHYNAISSNVFTDMGFGVGISGKWYWIVTHYGINVK"
     gene            complement(6734..7486)
                     /locus_tag="PK674_01210"
     CDS             complement(6734..7486)
                     /locus_tag="PK674_01210"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HOG15184.1"
                     /translation="MNIMSYLKDSHEKALAQNWLNSKFKILSCLLYGEINPRFFVTFD
                     NKKIAYLVISKNACTSIKAALIDNLVTGVDVHYSEKFKNTFNKLPSEKEDYFKFTFVR
                     NPFERAVSCYVNKFKEYKNRDNFFIFKKYLRGYLKEKDSFTAFINKISTIPDRFSDMH
                     FKSQYSFIYEKGKSVVDYCGKVENLKEDFAPIQKKYNLGSLIIQNKTSNKDRRSFYNE
                     DLAEKMYKRYKKDFDIRYPEEYEKLIHYLEGV"
     gene            complement(7505..8044)
                     /gene="cysC"
                     /locus_tag="PK674_01215"
     CDS             complement(7505..8044)
                     /gene="cysC"
                     /locus_tag="PK674_01215"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOG15185.1"
                     /translation="MSNIQRQPFTMLLTGLSGSGKSTVSDFLCKKIIEEYGVYVDQLD
                     GDKIRSTLNSDLKFSKNDRHKNITRVSKICDKLNKNGRSVIASFICPYASMRKIMKNN
                     IKNLIEVFLNCPLEVCEKRDVKGLYKKARNGEIKNFTGISDVFEIPENPDIILNTHKE
                     TITESVNKLINYLSKKYKI"
     gene            complement(8037..8660)
                     /locus_tag="PK674_01220"
     CDS             complement(8037..8660)
                     /locus_tag="PK674_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15186.1"
                     /translation="MLRNIEIDYKKYLNGKLDFQKKIGKIKFSNLIIISGITCVGKST
                     FIEKNKLPSLMTYKTGNNIKPHKKQIIINQKKFHKKMINGVFFDIYSINGNYYGYSIE
                     DYYILSRKYGNVFIEVSIESIYLGLRYIPKKIFILDYSEEQINKNIVKRIQERKYSKK
                     RINLLKKTIEKEKRFLEKIKEGSDLICLKIPLESRQQMNFINSNTNE"
     gene            complement(8645..9352)
                     /locus_tag="PK674_01225"
     CDS             complement(8645..9352)
                     /locus_tag="PK674_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15187.1"
                     /translation="MKKILIVGANSKISNEFIDTYVNRYKFYNTFKNKADLIHYKKNN
                     SQNYILDLNSIGSIRMFLQKVKKEYFSGVLLFSSIYKKDNFDDIGGMLENLNVNIINI
                     IYLIDKLIKNGNIVKQSKIIFFKDAGTKQPKCGYISYSIAKNIIGDILPSLSVKYNNI
                     IFLGIDMGPVYTDKIGGEKDIFYSKSLIKLDNPLSGLINFLNFLINEANFFSTGSIID
                     FSGGTYLIRNNQKCGET"
     gene            complement(9337..10119)
                     /locus_tag="PK674_01230"
     CDS             complement(9337..10119)
                     /locus_tag="PK674_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15188.1"
                     /translation="MNKIIRELKDLYLGGNKIKSYSAETINVLAHKDRVGFFVLNQKN
                     DNKYILYIYGNIKKNDIISIKQLFSKKMGYISVTVVDISGKYVRIKSNKKLDIDDFLM
                     YIPNFYIKNGWLFGPYLDNLLSILILNKINYKFNLWQSIDEEAKLIYSSFPKKGTTIV
                     LDVLRQEYLKEKINLNKIYCIRSTSFTSKLSTVVGKDIENIDLNLKFKLEVDCFDCKA
                     KYFLLCPIVNGHSAIASVKLSTLDKFEKTLNLLFNKIKGKKF"
     gene            complement(10112..11416)
                     /locus_tag="PK674_01235"
     CDS             complement(10112..11416)
                     /locus_tag="PK674_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15189.1"
                     /translation="MKNIKYYDVIDPNSEMNEQEIFIDDLLEKRHININSFNNNLSKY
                     KRLSDLSSGFGKQIIQDLLCCLGLKYDINNIKTLSSISPPLRKIISDNNKELLNIIDS
                     PDFLIPYLYMLIGFEINQSGLCGRNGSNPYESLAASIIRILNNDVIEISGFLCAHREN
                     KNISKNTLVENLNLYEFKRILSLINGIIKNLGKKYSINMMHTEGNYRGINAVFSNLFL
                     EYFKKGELNRMSDLLDQHIFELRTFESDNIKINSIGSHFDETKNFLEEKFGTDRINFE
                     MKDLYRLSEKDKFTSIAYYMTYEEIKRFDSALKSFNMAYTEDEINDLIKLNIRKASVN
                     KKETIEKAIKEFFNKSSLSAKALYETAFYYLRGKYVKEAGGIGMGFERDHDLFQTEAF
                     SMGYGPNKNKNMIPLLYGKRTGKKSGDSIGNISFRQFRHYYE"
     gene            complement(11430..12032)
                     /gene="folE"
                     /locus_tag="PK674_01240"
     CDS             complement(11430..12032)
                     /gene="folE"
                     /locus_tag="PK674_01240"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOG15190.1"
                     /translation="MIVYPSKSNGLTNNEKISIIEKHFFVIMETLGLDLDNDSLKKTP
                     YRVGKMLVEELFFGLDKNNEPSISTFDNKFNYDEIIIVKNIKVFSFCEHHFLPFVGNA
                     YVGYIPNGKVIGLSKINRIVNYLSRKPQIQERLNEEISEYMKKILGTDNVIVIIKAIH
                     YCMIMRGIKDINSSTVTSKADGCFRKSNKTRTEFMKLLNL"
     gene            complement(12221..12688)
                     /locus_tag="PK674_01245"
     CDS             complement(12221..12688)
                     /locus_tag="PK674_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="nucleoside deaminase"
                     /protein_id="HOG15191.1"
                     /translation="MQKEFMQEAIDLSKENIEKQFGGPFGAVVVKDGKVVGRGFNHVV
                     KNNDPTAHAEIQAIRDACTNLKTFGLSGCEIYISCEPCPMCLGAIFRARINKIYYGNT
                     QKDADEIGFDDQNFYKEFSKPIDQRSIPEVQLMYDEAKKVFDEWKEKVGSVEY"
     gene            complement(12806..13972)
                     /locus_tag="PK674_01250"
     CDS             complement(12806..13972)
                     /locus_tag="PK674_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOG15192.1"
                     /translation="MFLYKGLLYTIISTLFFFSFSFAFDFQTDCEEKLFIVTAYYSPK
                     DGQVFYYKENVVEEKILNGSGTHGASGRGVFNGMLAAPATYDFGGKILFPSLGVGEIA
                     DRGGAIVSAGKQGNRYDRIDIWMGYGEEGLIRALTFGKRVLKGYYCSNIKLKSLQANP
                     RVGFNFDNIPIPKNFFDATLFIQELREGRTDVRVYTLQQYLTKFGYMKKQTGFFGPET
                     KKALCNYQLKRGITSRKYCGVFGSATREYMKREAKRKGFLPDFGITATFDELISFAQN
                     YNGTNSSTQSTISGTESSQLSIINYFNQAYKKNVQNSKVSDLQDMLRHYGFFTGDLYD
                     KYDAKTIKAVYDFQLALKILSPNDTKNPARGWMGPSTRKALNEKRTEFQKHKSS"
     gene            complement(14000..14674)
                     /locus_tag="PK674_01255"
     CDS             complement(14000..14674)
                     /locus_tag="PK674_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="PHP domain-containing protein"
                     /protein_id="HOG15193.1"
                     /translation="MKILNTMSEDYHIHSINYSDGLNTIDEIVQYAHKIGLKKIIITD
                     HSQVALDAEKIELKNYRISLKRRVNVHNDLDVKFGVEGDLIDDEGNCCFEIGRNHSIE
                     EYFCILSCHDNAYKGDLKNITQAYINAIEKYHDKIKFIGHICNKKTSQYLDIPKIAEV
                     LNKYNIPIEINCSYLKLDRTDTSKLDQLLKLLKSGVYVNTDAHCLTDLHLKELGFQYL
                     KDRGYL"
     gene            complement(14757..15422)
                     /locus_tag="PK674_01260"
     CDS             complement(14757..15422)
                     /locus_tag="PK674_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="PHP domain-containing protein"
                     /protein_id="HOG15194.1"
                     /translation="MKVLNTMGEDYHIHSINYSDGMNTIDEIVQYAHKIGMKRVAITD
                     HSQATLEAGTRNYRVNLKRWKNMHNDLDIIFGVEGDLLDEEGNCCFEIGTKHSIEEDF
                     CILSCHDGVYQGDLKNITQAYINAIEKYHDKIKFIGHICSKKTSQYLNIPKIAEVLNK
                     YNIPIEINCSYLKLDRTDTSKLDQLLKLLKSGVYVCTDAHCLHDLQLKELGFQYLKDR
                     GYI"
     gene            15601..15762
                     /locus_tag="PK674_01265"
     CDS             15601..15762
                     /locus_tag="PK674_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15195.1"
                     /translation="MSGWPFDDKKNKENNGNSQYDGDVFETKTSYISKVAEFLESGIK
                     EMKKDIKKL"
     gene            complement(16094..>17225)
                     /gene="alr"
                     /locus_tag="PK674_01270"
     CDS             complement(16094..>17225)
                     /gene="alr"
                     /locus_tag="PK674_01270"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=25
                     /product="alanine racemase"
                     /protein_id="HOG15196.1"
                     /translation="MKRFLRKIFNPRVEPINRIFIYSKNVLNNLSYLQELQPKSAIFP
                     VLKSNAYGHGLLQITKILRKTNVPYIAIDSFPEYQVVKKYSDKNVLVLGETKPRNYKK
                     FDFSRTTFCVYNIETIAYLARLKKKIKIHLFLDTGMHREGVNEEELKNILEFLKDYPK
                     LIVEGVMSHLHSASSLDHNSIQTQIDFFKKMYHIVIDYGHNPKRRHIGNSAGIFKIQD
                     DFFNAYRPGLATYGYSPLLESDPYHKLTEKLKPALQIYSQITSLHQLLEGDGVSYNHS
                     RLADRDEFIASVPFGYTEGLPKSASNKIYFRHNKSYYPQIGTICMNICSMKISPKSYV
                     FDDVELIGLDGKNSLHYLSKASDKTVYEILVGLDKNIRRETV"
BASE COUNT         5970 a         2408 c         2200 g         6647 t
ORIGIN      
        1 gtcgcttaca tcctcgccac taaagatgac gaggttttac gctcccaata taaaatatgg
       61 ttttgttatt aaaacattta cttaactata gattctagga cttcaaagaa tttttctaaa
      121 tctgaaatat cgttgtacaa ataagcactc atcctacatg ttcatggaat ttttagatct
      181 ttatgcatag gataagcaca atgtccacca caacgaatag ctatattatg ttgagcaaat
      241 atttctcaaa tatggttgaa gttttcattt cctggtatga aaaaagaaaa aacagcggtt
      301 ctgtcattag tttttggtca aatcaatttt actttttctt ttaatttagt aaatctttct
      361 aatgaatatt caactaactt ttgttcatgt tctcgaattt tttccattca tcaaattgat
      421 ttgatgtatt ccaaggattt taataaactt attgctcaaa taatatttgg ggtaccagct
      481 tcaaattttg aatagtcgtt tggaactgta tgtcaaaaca aatctacatc tctgatagct
      541 catcacccag acatcatagg agtaagtttt ttgatccaat ctctttttag atacattgct
      601 ccaattcatg tgtaagccat aactttatgt gctgtaaaaa cgaaacaatc acatcatata
      661 tcttggacat ctattttcat atttgggaaa gactgggatc aatcaataat gaaaaaagta
      721 tctgctctaa gtagagattt tatttttttt acatcatata tttttccagt aacattggaa
      781 acatgtcaaa tagacacaac tttcacagat ttatcatatt tttttgaaaa atcatctcgg
      841 tcaatttcac atcattttga aactccaaca aaatctatct gaaatccaaa tattttggat
      901 aaaatttgtc ggggaactat atttgcatga tgttctcgta ttcataacaa aatcttatct
      961 cattttttta agaatcaaga gttgaccaag gattgtgcta taacattaat tcaatgagtg
     1021 gcattgtaat tgtatataat ttctgatgct ttactgttta agaattctgc aagaagttgt
     1081 tttgatttat cgtacaattc ctcagatttt tctgaaagtt gataagctca tctatgaata
     1141 tttgcataat catttgaagc gaaatcatta actccatcta ttacatattg aggcttttga
     1201 gtactagcag cactatcaag aaacaccaag gttggattgt tctgaaatat tggaaagtcc
     1261 tgttttttca tatttgatac tgtaaaaatc taaaagcagt atatgattct ggaagatacg
     1321 aaattatttt ttaactatta ttttatctca ttcaagagat cccaaatcta taattttcaa
     1381 agaatttgct tcattgtaat atttcatcaa aagattataa ttatctattt gacctgtaag
     1441 tgaattttta ttatttaaat atattgtttt cccgccaaca aaaacaactg tcatagaaga
     1501 tcatggcaaa tatacaattc tactatatcc gttaaatcat tgagaaataa catctatatc
     1561 agagatgagt ttttctggtg atatttcaac gaataatcaa tttatagatt ctgtgccact
     1621 taagtatttt ggtagctcaa cggtaaacac accatctcaa attgtattgc cactaaaaat
     1681 ttcaaaattg taatatcaca atactcaaaa tttcctgttt catagttgta aaaccatatc
     1741 taaatcatag aaaattattt gaactgaagc agaaaatttt tctggctgaa tgattcttat
     1801 gtttttcacg attgggaaat cttgttttag agtatgaaga atagcttttc ttttgaattt
     1861 ggatactaca taatagtttt catcttttat gagcttggaa atagtattgt acaaataagg
     1921 atcatcataa gtatctacac tagtttgact ataatagact ttttttatat aattttcgtc
     1981 tttaaaaata gtgtgctgta ttataaaaaa gattcaataa ataaaaatta tagataataa
     2041 aaatatccaa aatcgcttga taggaaagtt tatttccgga tagattaatt ttattttttt
     2101 aatctttctt tttatattat ttgttttcct tttttgaaaa ggcctatttt tgactaaagt
     2161 atacatagtt taattaaata aaatagtaat ataacacaaa agtaagagca atacccaaaa
     2221 ttattcctgc aaaaacttca aatggagtat gtcataacct ctcttttaag gactcttttt
     2281 gggtcattgt aagaacactc gtaatattca atctaatttc attaatgtat tctgcttgtt
     2341 tacctgattc aaatcttaga ttcatagcat cataaactac taatcaagca aaagcacatg
     2401 ccaatgcaaa atatatgcta tcaaaaccga attctatcaa aaccatgatc gctatgctag
     2461 aaacaataga tgagtgaaaa gatgggaatc agcccgaaga gaaaaaattg gaaatacaaa
     2521 attttttttg cttgatagaa tcaattgtta tttttataag ttgtgtaaca aatcatacaa
     2581 aaatcaggat aagtaatgga ttaattttta ttccttcttg gaaataagtt ttaaaacttt
     2641 ctcgaataaa tgaaaacatt gtttttttat aaaaatataa aaatacatca gtttatagtt
     2701 attgaaaata gtaaaagttt caagtgaaaa atttttttaa gaaagaattt gataggagaa
     2761 aaatttattt gtattgaaat aataaaatat ttacaataaa gaaatcggat atttttaaaa
     2821 aattttctac aatcttaaat ctatttttta atatatcatt atctgtagtt ttaaagctat
     2881 ttcattaatt tataaaaatg tttaatcctt tgataatcgc aatctgtatc agctttttct
     2941 ttgaaatata tttgtataaa agttatttta ttattctttt tagaaaaatt ataaatgatt
     3001 ctaataccag atcttgctca ttttcatttg agagatttac aagcaaattt tttaacttta
     3061 ataggacaaa aagtgtaatc atggactcac tgtcatgtaa ttgggaaaaa tcatcaattg
     3121 tttattcaat taacatgata tgtagacaag gcaaattgct taaatatgga aaaatcttca
     3181 tctaaagttt caaatttttt taacagtttt ttgaaatctt ttttaaattc atctatttca
     3241 tcaaagatta gtagcatatc tagttcaatt taaatactaa aatcatcatt ttcttcgtaa
     3301 tctctgatag aatattgtgg agatctatag aacacagctt cataaggaat ttctgcttga
     3361 tctgctgtgg aaattcgtgg tacatctcaa tgaacatatt cactaattct agatgctcac
     3421 atatctccta atttttgaat cacattatct aaaatttctt tttctgctca attaatcttg
     3481 gtaagatcgg cttttctaag agggatatat tttcttcatg tatgtcaatt tttaaaatga
     3541 atattttctt gttttatttc tttcttttcc ttcatttctt ttattatttt tattaattct
     3601 tttgaggttg gtcattgatg atttctaata tacctacttc ctataaaaaa ttcttcatat
     3661 ttttcgtaat aatcaaaatc acaaaaatat aagattttgt gcaaaacagt ttcactaaag
     3721 cttggtttag ctgaaagttt ttctaaaata tataaaaaaa cttctttaaa tttttcttct
     3781 tggcatgtgg gaatagacac tctaatttca tttttatcat ctttaataat tttataatgg
     3841 tctgtgtttt tttcattagt aaaaaaatat tcaatttgaa tttggaaaaa atcagctaac
     3901 tttttgagct catctacttt gatttctctc agtcaagatt ctatgttggt gtaaacaact
     3961 ctatccactc aaagaacttt ggataattct ttttgagaat atcaaaaagt ttttcttaaa
     4021 tcagctaact tttgagcaat aagtcttttt aaaatcattt agatatctta gataataaaa
     4081 ttatttatta ataattatag ttaatataat aatgtaagaa taaatttcaa tagatgttaa
     4141 attttattac cataatgaag ataaagattg tttttgaccg gagatgtatt gaacttagga
     4201 gcaattaggt agctctgcaa gtagtacaga taacggtgaa ataatgactt cttgtttgtc
     4261 gttaattaac aaaataaaaa acttgtttta agtatggata aatttataaa tattttgtgc
     4321 atttatatgt tttttgagaa aatgaaaaaa ttatataaat ttattgctgt tgttttgttg
     4381 gtgaatgttc tatttttcaa aacttataca tcaacatcag ctaacagccc aaaagatata
     4441 caaaaccaca agtacatgga aagtatacaa tatctctacg accattgagt tgttcaatga
     4501 tatcctgatg gaaactttcg tcctgatcgt gagataaatc gttgagaaat tatgaaaata
     4561 atattgaaat ctagtatttg accagactta tgagagttga ataattgttt tgaagatgta
     4621 aaagatgaac gatttgcaaa atatgtatgt tatgcaaaat cccaaaatat ggtaaaatga
     4681 tatgatgatt gaaaatttag accttatcaa tatgtgagtt ttgctgaagc attaaaaatg
     4741 tgaatagaat gattttcaaa aaagactgaa tcagtttcca actgagaacc ttggtatcaa
     4801 ggatatttga attttgttca caacaacaac attttttcta aatatgaaat atttccgtat
     4861 aaaaacatga ctcgttgaga aatggcttat tttattcatc aatttatgct agaaaaagag
     4921 tgaaaaatta gttttgggaa tcagcgcaaa ccttgatcaa gttgatgtgg aaaagagcca
     4981 ccagctacac caattaaatc atctgtagtt aattgagttg ttcgtaacta tatcactgta
     5041 atttgaaaca gatatgataa gaataaacca ataaaattaa tattagcatt tcatggtcgt
     5101 acaaattcaa acgaaatggt tcgtagttat tattgaatag aaaaggcgac aaactgaaat
     5161 gcaatcataa tatatcctag cggattacca gaaaacagtt cacctcgtaa tcgaagcaat
     5221 cctggcgaca aaaaatcaaa cttaagagat tttgctttgt ttgaccaaat actaaaagat
     5281 tttgaagaaa attattgtat tgataaagat caaatatttg tggtatgaca ctcgctatga
     5341 gcatggttta caaatagtct gagttgtgct cgtggagaca aaattcgtgc aatatgaagt
     5401 atttgatgat gaactacgat aaatgaatgt tctggtcctg tggcatgaat tataatgcaa
     5461 catcccgacg ataatttaag tccatataga gaatgagtgg tcgctcgtga tcaaatgtta
     5521 aaacaaaact tttgttgacc ggaaaccgtt ccattttgac cagcaccttt aaattgtgtg
     5581 aaatatacaa attgtctgtc tgatgctcct gtaatttggt gtccacattc agattctact
     5641 gataataggt gaaaatatta tcctcatact tggccagaca atgcgtgaag tgaaattata
     5701 aaattctttg aatcattgta gtttgtttta tttaacaaaa atcaatatga aaaagttttt
     5761 atttgttttt atctttggat ttgttttttt gaatttttta tgatttacaa atgcagaata
     5821 tccagtaagt gaaaaagtaa agactaggat tgatggcatc atgtctattg tagaaaagca
     5881 acgaggagaa aaaagtattt atcggcaaat ggataggtat caaaacatag taaacagttt
     5941 ttctttgttg aagaataatc ttgatggaga acaaaaagag atgatatgat atttagttta
     6001 tttgtttgaa aataagctta tcaaactaaa agaaatgtcc ataaatcaaa gcgatatatt
     6061 gaaaaatgta gatcgagaca gagttcaaga agaaattttg agatggcata atgaagaaag
     6121 gaataaatta tgattatcag attacataat aaatgataaa ctaaactact ctgctttagt
     6181 ttgggcagat aatttagcaa agaacaatag aaaaagcagt acacatacca gaaccccttg
     6241 agattgatat tataattacg ataaaatttt atcttggttt aataatttat gaatagattt
     6301 tgattataag cgaactgcat ttacagaaaa tgtagcttat caagtgtaca gttgtaataa
     6361 atctgattgt acacaagata tgattaatgc tttgaaaaaa tgatttgatt tttggatgag
     6421 cgaaaaaggt agatcttaca gacctcacta taatgcaata agtagcaatg tttttactga
     6481 tatgtgattt ggtgtttgaa tttcaggaaa atggtattgg attgtgactc actatggaat
     6541 taatgtgaag tagattagtt ttctattttt aacatagttt atttattctc atctctatat
     6601 tccatcaaca actttcaaag tatatttgtc ttaatctgtg tacaaagctt tttcttaatt
     6661 tattgtaaat aaaattttaa agagatatgt taaaaatttt tagctattct actctagacc
     6721 ccttaaaaaa acattaaact cattctaaat aatgtatcaa cttttcatat tcttctggat
     6781 atcgaatatc aaaatctttt ttgtatcttt tatacatttt ttctgctaaa tcttcgttgt
     6841 aaaaacttct tcgatccttg ttggatgttt tattttgaat aatcaatgat cataaattat
     6901 attttttttg tataggagca aaatcctctt ttaaattttc tactttaccg caataatcaa
     6961 ccacactttt tcacttttca taaataaaag aatattgtga tttgaaatgc atatcactaa
     7021 acctatctgg tatggtagat attttgttta taaacgcagt aaatgaatct ttttctttta
     7081 aatatcaccg taaatatttt ttaaaaataa aaaaattatc tcggttttta tattctttaa
     7141 atttattaac ataacatgaa acagctcttt caaaagggtt tcttacaaag gtaaatttaa
     7201 aataatcttc tttttctgat ggtaatttat taaaagtatt tttaaatttt tcactataat
     7261 gtacatcaac tcaagttact aaattgtcta ttaaggcagc tttaatagag gtacaagcat
     7321 ttttagagat aactaaatat gctatttttt tattgtcaaa tgttacaaaa aacctaggat
     7381 ttatttctca atataataaa caagataaaa ttttaaattt agaattcagc cagttttgag
     7441 ctaatgcttt ttcatggctg tcttttaaat aactcataat attcatttgt tatgttgcta
     7501 ggatctaaat cttatatttt tttgataaat aattaattaa tttattaaca gattctgtaa
     7561 ttgtttcttt atgtgtgttt aatataatgt ctgggttctc tggtatttca aaaacatcag
     7621 aaattcatgt gaaatttttt atttctcaat ttctagcctt tttgtaaagt cattttacgt
     7681 ctcttttctc acaaacttct aagggacaat ttaaaaaaac ttcaattaaa ttttttatat
     7741 tatttttcat tatttttctc atgcttgcat agggacatat gaaagatgct attacagacc
     7801 ttccgttttt atttaactta tcacatattt tagatactct cgttatattt ttatgtctat
     7861 cattttttga aaattttaaa tcactgttta aggtcgatct tattttatct caatcaagtt
     7921 gatctacata tacaccatat tcttctataa tttttttaca caaaaaatcc gaaactgttg
     7981 attttcaaga tcaagataat catgttaata acatagtaaa gggttgtctt tgaatattac
     8041 tcatttgtat ttgaatttat aaaattcatt tgttgcctgc tttctaacgg tatttttaaa
     8101 caaataagat cacttccttc ttttattttt tctaaaaatc tcttttcttt ttctattgtt
     8161 ttttttagga gatttattct ttttttagaa tactttctct cttgtattct ttttactata
     8221 tttttattta tttgctcttc agaataatcc aaaataaata tttttttagg aatatatctt
     8281 aatcataaat atatactttc gattgaaact tctatgaaaa catttccata ttttctactg
     8341 agaatataat aatcttcaat tgaatatcaa taataatttc aatttattga ataaatatca
     8401 aaaaaaactc agtttatcat ttttttgtgg aactttttct gattaattat aatttgtttt
     8461 ttgtgaggtt taatattatt tcatgtttta tatgtcatta aggatggcaa tttatttttt
     8521 tctataaaag tactttttca tacgcaggtt attcaagaaa ttatgatcaa attagaaaac
     8581 tttattttcc ctattttttt ctgaaaatct aattttcagt ttaaatattt tttataatct
     8641 atttctatgt ttctcaacat ttttgattgt ttctaattaa ataagttcat caagaaaaat
     8701 ctattataga tcatgtggaa aaaaaattcg cttcatttat taaaaaattt aaaaaattta
     8761 tcaatcccga caaaggattg tctaatttta ttaaagattt actataaaaa atatcttttt
     8821 caccaccgat cttgtctgta taaacaggtc ccatatctat tcataagaaa atgatattgt
     8881 tatattttac acttagagaa ggtaaaatat ctcatattat atttttagct atagaatacg
     8941 agatatatca acactttggc tgtttagttc atgcatcctt aaaaaagatt attttagact
     9001 gcttaacaat atttcaattc ttgattaatt tatctataag ataaattatg ttaatgatat
     9061 taacattaag attttctaac attcaaccta tatcatcaaa attatctttt ttataaatag
     9121 aagaaaaaag taggactcat gaaaaatatt ctttcttaac tttttgtaaa aacatcctta
     9181 ttgatccaat agaatttaaa tccaaaatat aattttgact attgtttttt ttataatgaa
     9241 ttaaatctgc tttattttta aatgtattat aaaatttgta cctatttaca taagtgtcta
     9301 taaattcgtt agatatctta gaatttgctc atacaattaa aatttttttc attttatctt
     9361 attaaacaat aaatttaatg ttttttcaaa cttatccaat gtagataatt taacagaagc
     9421 aatagcagaa tgtcaattaa caattggaca taataaaaaa tattttgcct tacaatcaaa
     9481 acaatctact tccaatttaa actttaaatt taaatcgata ttctcaatgt cttttcaaac
     9541 taccgtagat aatttactag taaaagatgt agatctgata caatatattt tatttaaatt
     9601 tattttttct tttaaatatt cttgccgtaa aacatcaagc actattgttg ttcacttttt
     9661 cgggaaacta gaataaatta gtttagcttc ttcatcgata gattgccata aattaaattt
     9721 ataatttatt ttatttaata tcaagatcga taaaagattg tccaaataag gtcaaaacaa
     9781 ccatcaattt tttatataaa aattaggtat atacatcaaa aaatcatcta tatccaactt
     9841 tttattagat tttatcctta catatttacc gctaatgtcc acaactgtaa ctgagatata
     9901 tcacattttt ttcgaaaaaa gttgtttaat ggatattata tcgttttttt taatatttca
     9961 gtaaatatat aaaatatatt tattatcatt tttctgatta aggacaaaga atccaaccct
    10021 atctttatgt gccaaaacat taatagtttc cgcagaataa gattttattt tgttacctca
    10081 caagtataaa tcttttaatt ctctaattat tttattcata ataatgccga aattgtctaa
    10141 aagatatatt tcaaatggaa tctcaacttt ttttaccagt ccttttccca taaagtaatg
    10201 gtatcatatt tttattttta ttaggtccat atcacataga aaaggcctcc gtctgaaaca
    10261 aatcatgatc tctttcaaat cacattcaaa ttcatcaagc ctcttttaca tactttcatc
    10321 gtaaataata aaatgctgtt tcatacaatg cttttgcaga taaacttgat ttattaaaaa
    10381 attctttaat agccttttca attgtttctt ttttgtttac cgatgctttt ctaatattta
    10441 attttataag atcattaatt tcatcttcag tataagccat attaaaagat tttaaagctg
    10501 aatcaaacct tttaatttct tcgtatgtca tataatatgc aatacttgtg aatttatctt
    10561 tttcagataa tctatacaaa tccttcattt caaaatttat tcgatctgta ccaaattttt
    10621 cttctaaaaa gtttttagtt tcgtcaaaat gagatcaaat agaattaatt tttatgttat
    10681 cggactcaaa agttctcaac tcaaaaatgt gttgatctaa caaatcagac atcctattta
    10741 actctcattt tttaaaatat tcaagaaata aattggagaa aaccgcattt attcatctat
    10801 aatttcattc tgtatgcatc atattaatag aatatttttt tcataaattt tttattatac
    10861 catttattag acttaaaatt cttttaaatt catataaatt taaattttca accaaagtgt
    10921 ttttggatat atttttattt tctctatggg cacataaaaa tcatgatatt tctatcacat
    10981 cattattgag aattctaata atagatgctg caagggactc gtatgggtta gatcagtttc
    11041 taccacacaa tcaagattga ttaatctcaa atcatattaa catatacaaa tatggtatga
    11101 gaaaatcggg agagtcaata atatttaata gttctttatt attatcagat attatttttc
    11161 ttaatggtgg ggatatagaa gaaagtgttt taatattgtt aatatcatac tttaagccta
    11221 aacaacataa taaatcttgt attatttgtt taccaaatca tgatgataaa tctgataatc
    11281 ttttatattt tgataaatta ttattgaaag agttaatatt aatatgtctt ttttctagta
    11341 aatcatctat aaaaatttct tgctcgttca tttcagaatt tggatcaatt acatcataat
    11401 attttatatt tttcataatt taatttaaat tataaattca ataatttcat gaattctgtt
    11461 cttgttttat ttgattttct aaaacatcaa tctgctttag atgtaacagt agaagaatta
    11521 atgtctttta ttcatctcat aatcatacaa taatgaattg ccttgattat aacaataaca
    11581 ttatctgttc ataatatttt tttcatatat tcagatattt cttcatttaa tctttcttga
    11641 atctgtggct ttctagaaag atagtttact attctattta tttttgataa tccgataact
    11701 tttcaattag gtatataacc aacataagca tttcaaacaa aaggcaaaaa atgatgttca
    11761 caaaaactga acactttaat atttttaact attattattt catcataatt aaatttatta
    11821 tcaaatgtag aaatagatgg ttcgttattt ttatctaatc aaaaaaacaa ttcttcaacc
    11881 aacatttttc acactctata aggagttttt tttaagctat cattatctaa atctaatcaa
    11941 agagtttcca taattacaaa aaagtgtttt tcaataatcg aaattttttc attattagtt
    12001 aatccattgg atttagaagg atacactatc atataaatat aaatatataa aactattgtt
    12061 agtatataat attattataa taaatgtcaa gaaataattg tcaagaaatt attattaaaa
    12121 ttatttctat tcttacatat tactattcta aaaaatctaa tttgtaaaaa taatatttct
    12181 tatttaattc tggtgataaa tttttaaaat gtaaattctt ttaatactca acgcttccta
    12241 ctttttcttt ccattcatca aaaacctttt ttgcttcatc atacatcaat tgaacttctg
    12301 gaatagatct ttgatcgatt ggttttgaaa attctttata aaaattttgg tcatcaaaac
    12361 ctatttcatc tgcatctttt tgtgtatttc agtaataaat tttatttatt ctagcccgaa
    12421 aaatcgctca caagcacatt ggacatggct cacatgaaat ataaatttca catcatgata
    12481 aaccaaaagt ttttagattt gtacaagcat cacgaatagc ttgaatttca gcatgagcag
    12541 tagggtcgtt gtttttgaca acatgattga atcatcttcc gactactttt cagtctttta
    12601 cgactaccgc accaaaaggt cagccaaatt gtttttcaat attttctttt gaaagatcaa
    12661 tagcttcttg cataaattct ttttgcattt ggattaagct tatcaaataa aaggtgtcta
    12721 cttgtaaagt tacaagttag atccctacca cccgggtgca attggctagc ttcgcgaaca
    12781 gcgcccgggt ggtaggggtg taactttacg aacttttatg cttctgaaac tctgttcgtt
    12841 tttcgttcaa ggcttttcta gtggaaggtc acatccatca tctagcagga ttttttgtat
    12901 cgtttgggga taatattttt agtgctaact ggaaatcgta aacagctttg attgtttttg
    12961 catcatattt gtcatataaa tctcatgtaa aaaatccata atgtctcaac atatcttgta
    13021 aatcagaaac ttttgaattt tgtacatttt ttttgtaagc ttggttaaaa taattaatga
    13081 tcgataattg agaactctca gttcaagaaa ttgtgctttg tgtagaactg tttgttcagt
    13141 tataattttg agcaaaagaa attaattcgt caaatgtagc tgttattcaa aaatctggta
    13201 aaaatcattt tcttttggct tctcttttca tatactctct ggtcgccgat caaaaaactc
    13261 aacaatattt tctactggta attcaccttt tgagctgata attgcaaagt gctttttttg
    13321 tctctggacc aaaaaatcaa gtttgtttct tcatataacc aaacttagta agatattgtt
    13381 ggagagtata aactcgtaca tctgttcttc attcacgaag ttcttggata aacaatgtcg
    13441 catcaaaaaa attttttggt attggaatgt tatcaaaatt aaatcaaacc ctgggatttg
    13501 cctggaggga tttgagcttg atatttgaac aataatatca ttttaatact ctttttccaa
    13561 aagttagagc tctaataagt cattcctctc aatatcacat ccaaatatct atacgatcgt
    13621 atctatttca ttgttttcat gctgaaacta tcgcccctcc tctatctgct atttctcaaa
    13681 ctcaaagcga tggaaaaagt atttttcctc caaaatcata agtggctggt gccgctaaca
    13741 ttccattaaa aactccccta ccacttgctc catgagttcc acttcagttt aatatttttt
    13801 cttctactac attttctttg tagtaaaaaa cttgtcagtc ttttggagaa taatatgctg
    13861 taacaataaa aagcttttct tcacaatcag tttgaaagtc aaaagcaaaa gaaaatgaga
    13921 agaaaaagag tgttgaaatt attgtatata ataagccttt gtacaaaaac atttcgaaat
    13981 ttaaaaataa atattacagt taaagatatc ctctatcttt caagtattga aaaccaagtt
    14041 ctttgagatg taaatcagta agacaatgag catccgtgtt tacatacaca ccagatttta
    14101 gtaatttgag caattggtct agtttggaag tatctgtcct atctaatttt agataagaac
    14161 aatttatttc gattggtata ttgtatttat ttagaacttc tgctattttc ggtatatcga
    14221 gatattgaga tgtcttttta ttgcagatat gtcaaataaa ttttatttta tcatgatact
    14281 tctctatagc atttatgtat gcttgggtaa tatttttcaa atctcattta taagcattgt
    14341 catgacaaga taatatacaa aaatattctt ctattgaatg atttctccca atctcaaaac
    14401 aacaatttca ttcatcatcg atcaaatctc attccacacc aaacttaaca tctaaatcat
    14461 tatggacatt tactcgtctt tttaaagaaa ttctataatt tttaagctca attttttctg
    14521 catctaatgc tacctgacta tgatcagtaa ttattatttt tttcagtcat attttatggg
    14581 catattgtac tatttcatct attgtattta acccatcaga gtaatttata ctatgtatgt
    14641 gataatcctc actcattgtg tttaatattt tcattctatt ctagaaaaca atataaatct
    14701 aaaatgacat aaaaaaccca gtcagtaatt aaaatccaaa agaacaaaaa ttatgattaa
    14761 atatatcccc tatctttcaa atattggaaa ccaagttctt taagttgtaa atcatggaga
    14821 caatgtgcat ctgtacacac ataaacacca gattttagta atttgagcaa ttgatctaat
    14881 ttggaagtat ctgtcctatc taattttaga taagaacaat ttatttcaat gggtatatta
    14941 tatttattta gaacttctgc tattttaggt atattgagat attgagatgt ctttttgcta
    15001 cagatatgtc aaataaattt tattttatca tgatatttct ctatagcatt gatatatgct
    15061 tgagtaatat ttttcaaatc tcattgatac acaccatcat gacaagacaa tatacaaaaa
    15121 tcttcttcta ttgaatgttt tgttcaaatt tcaaaacaac aatttcactc ttcatcaagc
    15181 aaatctcatt ccacaccaaa tataatatct aaatcattat gcatattttt ccatcttttc
    15241 agattaactc tatagtttct agttcctgct tctaatgtcg cttgactatg atcggtgatc
    15301 gccactctct tcattcatat cttgtgggca tattgtacta tctcatctat agtattcata
    15361 ccatcagagt aatttatact atgtatgtga taatcttcac ccatcgtgtt taatactttc
    15421 atcttatttt ttagaaatat aaatctaaaa taatataaaa agatctaatc aaaaatcaag
    15481 acaccagagt ccaataaaaa attacttgaa aatcgagtga tatttattag agtgatgttg
    15541 tttatgaatt aataattcaa tatgttcttg tgactaacaa atcgcctaat aaattttatt
    15601 atgtcaggat ggccttttga tgataaaaaa aataaagaaa ataattgaaa tagtcaatat
    15661 gattgagatg tatttgaaac aaaaaccagt tacatatcca aggtcgcaga gtttttggaa
    15721 agctgaataa aagaaatgaa aaaagatatt aaaaagcttt agtattttaa gtttgtaagt
    15781 acaaagttgg tagttatccc cttttgtaac aactaaggga gcttcattat tctcaaatct
    15841 tcctagggag gaattttatc gtgaaattct tccctcgggg gaagctgggc ccccgcagta
    15901 ttgcggggta gatggggggt tgtagttggg ggtaaaaccc ccacctatcc cgatgaatcg
    15961 ggataacacc ctcccccaag ggaggaattt tatttaagga tttaagaact ttaagaactt
    16021 gatgaaccac gccccgcccc gcagtattgc ggggcggggc gtggcaggct ttacaaactt
    16081 gacgaatttt tcactacact gtctctctac ggatattttt gtccaatcat actaaaattt
    16141 cgtatactgt tttatctgac gctttactta aataatgaag cgagtttttt caatctaatc
    16201 aaatcaattc tacatcatca aacacatatg attttgggga aattttcata gaacaaatat
    16261 tcatacatat cgttcatatt tgaggataat aagatttatt gtgcctaaag tatattttat
    16321 tactggcaga tttgggtagt cattcggtat atccaaatgg tacagaggca ataaattcgt
    16381 ctctgtcagc taatcggctg tgattgtaac taactcaatc tcattccagc aattgatgta
    16441 atgatgtgat ttgggaataa atttgcagag ctggttttag tttttcagta agtttgtgat
    16501 acggatctga ttctaataat ggagaatatc cataagtggc tagtcaaggt ctataagcat
    16561 taaaaaaatc atcctgaatt ttaaatattc ctgcactatt tcatatatgt ctccgtttgg
    16621 gattatgtcc ataatctatg actatatggt acattttttt gaaaaaatct atttgggttt
    16681 gaatagaatt gtgatccaaa gaacttgcac tatgtaagtg ggacatcact cactctacta
    16741 ttaattttgg atagtctttc aaaaattcta agatattttt tagttcttct tcatttactc
    16801 attctctgtg cattcaggtg tccaaaaaaa gatgaatttt tatttttttc ttgagccttg
    16861 ctaaataagc aatagtctct atattgtaca cacaaaatgt tgtcctggaa aaatcaaatt
    16921 ttttataatt cctcggctta gtttctcata atactaaaac atttttatca gaatacttct
    16981 ttaccacttg atattctgga aaactatcaa tagctatata tgggacattc gtttttctca
    17041 aaatttttgt tatttggaga agtccgtgtc cgtaggcatt ggattttagt acagggaaaa
    17101 tagcagattt tggttgcagt tcctgtagat aagacagatt gttgagaaca ttttttgaat
    17161 aaataaaaat tctattgatc ggctctactc ttgggttaaa tatttttctt aagaatcttt
    17221 tcatg
//
LOCUS       DAOSID010000011        14860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_4164_length_14860_cov_17.335562, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000011 DAOSID010000000
VERSION     DAOSID010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 14860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14860
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4164_length_14860_cov_17.335562"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PK674_01275"
     CDS             complement(<1..906)
                     /locus_tag="PK674_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15197.1"
                     /translation="MFIISFAIFVFVLTVFENVFLALLLLILLLLGHSIVKKFRNTAR
                     TSLLITLAFLISILSIFVKNYKYDQDIKSLPNVEGRISRDMDGPVGKYFVGTGIVSDI
                     YSYQKYVFQDNAGREYFLISSRKFEIGDILWLNGRVDLAYTGAGNIFDFQKQWNDFVS
                     KPNLSGLFDYEFNYSKWLMMKGFYGTIYGQSYLVFKDECEYHNFADDVFETEDFLPPS
                     SFAHNFPQGKNVSLVAQIYPPFLAPLHSLSGTKGLKKSLYPPVAPQYCGAVRGEQLPL
                     IKGDNFSGKGNDENLHSLSEQNCKSK"
     gene            complement(1010..1939)
                     /locus_tag="PK674_01280"
     CDS             complement(1010..1939)
                     /locus_tag="PK674_01280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOG15198.1"
                     /translation="MKLYIKGFYGYRNFGDEMLFFGLLNYLQREYNPESFTVEVGDLQ
                     RIQTRIQHNKTWLEPGILDKLDFVENAVISRRFRQLQIFLGLDKYRKYFKVFGGGEVL
                     DESRKFPHDGWNLKILYRHDIRKKNFILVGGIGTDQKKYTKKLFKYMFKKTKRVICRE
                     KTSYERAVQYGAKSAVLYQDFSKDILEDFKNKNPVSLSSTVLINISPKHFTDDNIEKI
                     KKFVLKYPDHKKIFFPADINFDKEFYSVLRKVIPDLVVYDWTKHSLLDTLKLFQSCDA
                     GIGSRLHFLYPLKVFEKEFVSLSSSDKVSKFIK"
     gene            complement(2039..6370)
                     /locus_tag="PK674_01285"
     CDS             complement(2039..6370)
                     /locus_tag="PK674_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="thermonuclease family protein"
                     /protein_id="HOG15199.1"
                     /translation="MKKILSVLLSILILFQSVFSSSFVRGDKVENYKEPQQESSNLSD
                     DFFENKGSLENTEPDDDFWGPITKGGQTGQLLDNNLFVGVNLENQDLNGEYFVGEGQN
                     EQTGFWDDTTNGEIINESCMDDDFGNDEIFGEENFIPLVAQLTTEGNGNIVFDVSFAD
                     GRANLPTGRTAYGLGSDYTSTAPKLKFDSSGDRLKSPEFILTTGAQATVRTKANGSSV
                     PGGSVFSGKAIFTDQNDEVIFEFADFLPNKEKNIIFSVSNTVTQITLTYEKGIGNLGV
                     GSFKLEYLNEYSGTEPNIPQLKTTEIYFDGDENRFEITNIGNDTFSGELSLSGNLNFT
                     FSTNIPSGVSKVFANSLYSMFQTGKNIQTIPNSINFTNEEFNLDLIRSGQVLDNFFVH
                     SSYVDYYQDYKTSFEKIGTGDSWTTTVVGLNSDRYYNINQGIAANPTKYFTTGENLID
                     VTLDKNPYNPDPGNTGSDLNIPIDCDDFYADWILDISEVYYGSGIYPSYVELNITDDV
                     GDDYPQIKLGGSALDQEIVFSSTELQAGKRFLITDSDVRYNEGRDSLYDSNFSLNSSG
                     WLIVYGKDGADLEVLDVVYLNGGIQGASLYMGTDFIQCAGVFDYVDKFSPGMTIGQSQ
                     FIQISPEPVVQYIKVNNGGGFCPQAEGFQFNTNDSTNGDIQISSLKYYGNYQILKLKN
                     KTNSDINLRDYKIQGFDGEIKSIKGNTLFAKQTMSFVGNYGFPTKTDHCINLIKDGEI
                     VDRYCRNSMSKASETDEENIQNQLEFWIDIDEEEIDEELEEEVFTGNIASLKNIKIKH
                     IDYDPPGSDKDNETITLLLLTGTQVDLSKYTLQYIKEGKATNKSLKGILTFGAEQTFK
                     GDYTLPNSTQDKNPVIVHILNPNQQIVDTYIYNPNKIKEIPDGEYKVISIIDGDTIKI
                     SYLEQEFNIRLAGIDAPESSALRCGKVECFGPEAKEYLNSLLANKTISFQQAEKDDFD
                     RFVGYVFLNGENINEKLIRNGYAREYSYKGKNYKYQSLFQSAQKYAQQNSLGLRASPC
                     FGKRLCPVEETKILDDYEFTIDYVLYDPEGDDKDKEEITITMHKGFPIQFSEGFYLMV
                     NTTKKSLKNYGGISPNETKTLRGTFGFPNTKATTVSLMYEDTIFATYSYDPEKDKLLG
                     KEENELTGVDIKITGLIPNPKGKDSLGESVRLLYTIDPSVLHSSIDSLSTNDENESDP
                     SLLSQGYSKNINLADGFYLKIGSAKKKLNGMLVPDQEILVTGNFSLPNKAACVEIGYK
                     NIIFDKFCYPHPTEGQKFLVSNGVLESIVDDDFGILKTAKLQNFGNQVCLTYGGQKFY
                     CKNMPYSKLSTKRLNQNKLYKEYFDVFEEYLKSNWRVMYYNSEIKNYFDLLNQIEKAI
                     SNGDSTIEFDGNVYSTNEFKAMYESQYPSTVSSSLNQTINELVPSPIVQKYQQLKEEY
                     LEYLFNL"
     gene            6951..8105
                     /locus_tag="PK674_01290"
     CDS             6951..8105
                     /locus_tag="PK674_01290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="glycosyltransferase"
                     /protein_id="HOG15200.1"
                     /translation="MKKKHIAMAGGGTGGHVFPIKSLIEHIHKNPKYSQKISKIIRFG
                     QKKSLEKELFDELKQKYPLLYFQNIVSGKYRRETWFSSKLKNVRDIFKFMFGIFQSIF
                     YLVIHRVDVVFCKGGYVALPVVFAARFLRKKIVVHESDVRSGLVNKIAARYANRVFTG
                     FDGVFVKSTTVGQILSDEIISKQSIKNPPKVRDISQKNIELTIQEQLFYSDPSKTYLL
                     VMGGSQGSKRLYQDLQNTIVSNPEIFKNYEIFVVLGKENVQQKELFEKIKNVHTFDFL
                     SQSEMGLLLKHCDISLTRAGTTSLAEQKLYNLKIIMVPIPWTHDQYDNAKYYVKHYDD
                     ILLDSKDPDYKNNMLKIFDDYKNFKKSEQNVDILSVISKAKDVIVNELIS"
     gene            complement(8102..8554)
                     /locus_tag="PK674_01295"
     CDS             complement(8102..8554)
                     /locus_tag="PK674_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG15201.1"
                     /translation="MKHPGEFLIEEIRARGLTQKDFSLMLGKSGSELNELIKGKRNIT
                     IQWDILLSQVLGSPEKYWIHKQIDYDYFLAKKESENLKSKVEQDIILDRDLGKVDPVL
                     EGDLVEELRNDSKISVGFDKEITKKEPYLKKYDEEYSKSKIENIFRDF"
     gene            complement(8877..9527)
                     /locus_tag="PK674_01300"
     CDS             complement(8877..9527)
                     /locus_tag="PK674_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOG15202.1"
                     /translation="MKITVYEDSFFYYFWKPHSIPSTFGKEKCFLDFLLESKDQYIKS
                     ILFYLDDMFGKEQEYGLLNRLDNDTAGLLYFAKTPLVKEKYKQAQFKGKITKYYVADV
                     YGKFAPNSLKISYLIGHHKFSKDRMVVFLDRKQIGKIDKKRTHQVDTNVEKLYYNKNT
                     NTTTLLVKISKGVRHQIRAHLSSIGFPIVGEKIYIKKSGSEKLQLFCIGMNIKKTP"
     gene            complement(9662..10618)
                     /gene="secF"
                     /locus_tag="PK674_01305"
     CDS             complement(9662..10618)
                     /gene="secF"
                     /locus_tag="PK674_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="protein translocase subunit SecF"
                     /protein_id="HOG15203.1"
                     /translation="MKARFNIIKKAPIWIAISIILFLASIFFFFSNARYSEEFTGGIS
                     LGIDKILDNQKEQESLKQYLLGHQYKDLRVSLETIENTTTFKINTKVDTDEKVAELSD
                     EIKQYLLENNLISSTDEIIEQTITGPSVGSYMQKSAITALIVGVIFIVIYMLFSFSAI
                     RNYVSPSILGLVVAITMIFDVAIPAGAYGVWMWLNNTIQIDSIFIIAILTIMGYGIND
                     TIIIFDRVRENLQNKGTGKDVIYGRVFEDSIWQTMKRSVGTSFSTLLIVIAMFVFGTG
                     IMKHFAFTIGVGIISATFASIFIATSLVYILLGKYKKEKGKF"
     gene            complement(10936..11661)
                     /gene="rnc"
                     /locus_tag="PK674_01310"
     CDS             complement(10936..11661)
                     /gene="rnc"
                     /locus_tag="PK674_01310"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="ribonuclease III"
                     /protein_id="HOG15204.1"
                     /translation="MLNKKVLEQKEKIYSYIKELGLDPSKIKNEGLLLQAFVHKSFAA
                     DFKKILGHNERLEFVGDGVLGAIVCKLLFQKHPKMDESEMTLYKIALVREGNLAQVAR
                     NINLDKHILVSRGEEKTQGRKKDAILADALEALIGFVFLDFGYAQAEVFVEKNVYTMY
                     NKISKKPIQSYKTMVQEHLQKLYKELPSYKDIEHEIDSKGNVLTYKAEIYVLGVKKAE
                     GFGTNKKKAQEDAAQNFYESIKA"
     gene            complement(11686..13152)
                     /locus_tag="PK674_01315"
     CDS             complement(11686..13152)
                     /locus_tag="PK674_01315"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="LCP family protein"
                     /protein_id="HOG15205.1"
                     /translation="MGAKFKQSKVGEIHETDSKQNKIFSKILKYIVFLICLSLIIFFG
                     KNLIHGGHVLLGSLGKSTVKTVSSNLGQEMRRDEFGNVNIMLVGIGGDDHHGGYLADS
                     MIATSRNPELGAVTMISIPRDLYVNSSGYLGRINGLFARGYSRGGNNIGSGAENLIAK
                     VEEILGLEIPYYLVADFQGFKEIVDTLGGIDIYIENTIHDITYPDDNLGYQTFHISAG
                     QQTLDGDTALKYVRSRHTTSDFARSQRQQNVIKAVIDTALQRKNITNVGKLKEMYSTY
                     TRMVYTNISSKEMVGMFRYIYDFKHIFSFGLNTYCTYSSYKLTDAGCFLYNGNREAFG
                     GQAVMVPNGAKSNNISFYDYIQRFTFFVTHNNGYLVENPRILIKNAIDKNFASQHRLS
                     PTGRANKIAVKLKKYGFNLAGIENHSGTIEQTKVVTYGDQNYSKTIDTLQYFFPVNVV
                     EKGENQFITGEELNYDMELIIGNDFISYTANTPFSYER"
     gene            complement(13163..14332)
                     /locus_tag="PK674_01320"
     CDS             complement(13163..14332)
                     /locus_tag="PK674_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15206.1"
                     /translation="MKKYFIILLFPVIILSACQKNMYLEMDFNQAISVFTQQISYILN
                     SINDLGFSGQKETQTKLEIDGENADFLLNSNIELNAYTNYLANKQDLHNQISLHFLDK
                     KEKNILNLSGNIAYKNIENERYINLQDFFIDLGSGNYQNNLLLLISKKLENKRMFLDL
                     KEKSNTNFYKDFSFLLQTILASNIFYPINELKYNGFFAYKIKIEQDVVDYISDNTNFK
                     INFFEGLLIIKSDSDIELKIENLELYYNDKNYTIKGNIDDKNSDLVIKIPEKEGTTYA
                     VSLSFHKSQCKFQLKQLANYQDMISLNINIINKKSENFIKNIINGNIEVSNKFIYGTD
                     LEKNIKINIKAEQKIIEDFGFVFKKPDSYIMLKQILGDSFSLNHILSTGDIFINK"
BASE COUNT         4941 a         2414 c         2073 g         5432 t
ORIGIN      
        1 cttgcttttg caattctgct ctgaaagaga atgtaagttc tcatcattac cttttccaga
       61 aaaattgtct ccctttatca aagggagctg ctctccccgc accgccccgc agtattgcgg
      121 ggctacagga gggtagagag atttttttaa tcctttagtc cccgaaagac tgtggagcgg
      181 agctaaaaaa ggaggataaa tttgagccac aagcgagaca ttcttccctt gggggaagtt
      241 gtgtgcgaag ctagatgggg gtaagaaatc ttctgtttca aaaacatcat cagcaaaatt
      301 atgatactca cattcatcct taaaaactaa ataactttgc ccatatatcg taccataaaa
      361 ccctttcatc atcaaccatt tggagtaatt gaactcataa tcaaatagtc atgacaagtt
      421 cggtttgctg acaaaatcat tccattgctt ttgaaaatca aaaatatttc ccgctcccgt
      481 ataagctaga tccacccttc aatttaacca caaaatatct caaatttcaa attttcttga
      541 agaaattaaa aaatattcac gcccagcatt gtcttggaaa acatattttt gataagaata
      601 aatatcggaa acaatacccg ttcctacaaa atattttcaa acaggtcagt ccatatcacg
      661 cgaaattctt cattctacat tgggcaaaga tttgatgtcc tgatcgtact tataattttt
      721 gacaaaaata gacagaatag aaattagaaa tgctaatgta attaacaagg atgtacgagc
      781 agtatttcta aattttttaa caatagaatg tcaaagtaac aatagaatca acagcaacaa
      841 agctaaaaaa acattctcaa aaaccgttaa aacaaataca aatatcgcaa atgatattat
      901 gaacatggat tgtattattg attaattaaa tcatcttcta tcctaccacc cgggtgcaaa
      961 attgaccccg ggataactcc cggggtcaaa cagcacccgg gtgatcttct tacttaatga
     1021 acttactcac cttgtccgaa ctagagagag acacaaactc tttctcaaaa acctttaatg
     1081 ggtacaaaaa atgcaatcta gacccaatcc ctgcatcaca agactgaaac aatttcaagg
     1141 tgtccaataa ggaatgcttc gtccaatcat aaaccaccaa atctggaata acttttctta
     1201 agacagaata gaattcctta tcaaaattaa tgtctgcagg aaaaaatatt tttttatgat
     1261 ctggatattt caaaacaaat ttctttattt tttcaatatt atcatctgtg aaatgcttcg
     1321 gagaaatgtt gatcaaaacc gtggaggata aagaaactgg atttttgttt ttgaaatctt
     1381 ctaaaatatc tttagaaaaa tcctgataca aaaccgctga tttcgctcaa tactgaactg
     1441 ctctttcata agatgttttt tctctacaaa taaccctttt tgttttctta aacatatatt
     1501 tgaacaattt ttttgtatat tttttttgat ccgtaccaat tcatcacact aaaataaaat
     1561 tctttttcct aatgtcatgg cgatacaata ttttcaaatt ccatcaatcg tgtgggaact
     1621 ttctagactc atctaaaact tctcatcacc caaaaacctt gaaatatttt ctatatttat
     1681 ccaatcaaag aaatatctgt aattggcgaa acctcctgct aatcaccgca ttttctacga
     1741 aatctagttt atctaaaatt catggttcta gccaggtttt gttgtgctgt atccgggtct
     1801 gtatccgttg caaatctcaa acctctacgg taaaagattc tggattgtat tctctttgca
     1861 aataatttaa cagaccaaaa aatagcattt cgtctccaaa attcctatag ccataaaatc
     1921 atttgatata aagtttcata tgaatgaatt aaggttaaag attctacagt accacccggg
     1981 tgcaaaatag accccgggag ttatcccggg gtcaaagcag cacccgggtg gtaagatatt
     2041 ataaattaaa taaatactcc aaatactctt ccttcaactg ctgatatttt tgaactatcg
     2101 gtgacggaac aagctcatta attgtctgat tcaaagaact tgaaaccgtt gatggatact
     2161 gagattcata catcgctttg aattcgtttg tactataaac attaccatca aactctattg
     2221 tagaatctca attacttata gctttttcta tctgatttaa caagtcaaaa taatttttta
     2281 tctcgctatt gtaatacatc actctccaat tactctttag atattcctca aaaacatcaa
     2341 aatactcttt gtaaagtttg ttttgattca atctctttgt agacagttta gaataaggca
     2401 tatttttaca ataaaacttc tgtccaccat atgtcaaaca aacctgattt cagaaattct
     2461 gtaatttcgc tgtttttaat atgccaaaat catcatcaac gatagactcc aaaactcaat
     2521 tagataccaa aaacttttgt cattctgttg gatgaggata acaaaattta tcaaaaataa
     2581 tatttttata tcatatctct acacaagctg ccttgttggg taatgaaaaa tttcatgtga
     2641 ctaaaatttc ttgatctggg actagcattc aattaagttt tttcttggcg cttcagattt
     2701 tcaaataaaa tccatcagcc aaattgatat tttttgaata tcattgtgaa agtaaagaag
     2761 gatctgactc attttcatcg tttgtggaca aagaatctat ggatgaatga agtacagacg
     2821 gatcaattgt gtaaagtaat cgtaccgact ctcaaagcga atccttccct tttggatttg
     2881 ggattagtca agtaattttt atgtctactc ctgtcaactc attttcttct tttcccaaaa
     2941 gtttatcctt ctctggatca tagctatatg tggcaaaaat tgtatcttca tacatcaaag
     3001 aaaccgtagt agctttggtg tttgggaagc caaaagttca tctcaaagtt tttgtctcgt
     3061 ttggcgatat tcatcaataa tttttcaaag attttttagt agtatttacc atcaaataaa
     3121 atcattccga aaactgaatt gggaatcctt tgtgcatagt gattgtaatt tcctccttgt
     3181 ccttatcatc tcactccgga tcatacagaa catagtctat ggtaaactca taatcatcca
     3241 aaattttagt ttcttctaca ggacacaatc tttttcaaaa acatggagaa gcccgcaatc
     3301 acaagctatt ttgttgagca tatttttgag ctgattggaa taatgattga tatttatagt
     3361 tttttcattt atatgaatac tctcgtgcat atcaatttct gattaatttt tcgtttatat
     3421 tttctcagtt caaaaagaca tatcacacaa acctatcaaa atcatctttt tctgcttgct
     3481 gaaacgaaat cgttttattt gcgagtaggg aattaagata ttcttttgct tctggcccaa
     3541 aacattcaac tttcccacat ctcaaagcag aagattctgg tgcatctatt caagcaagtc
     3601 tgatattaaa ctcctgttcc agataagaaa ttttgatagt atctccatcg ataatagaaa
     3661 taaccttgta ttctccatct ggaatttctt tgattttgtt tggattgtaa atgtatgtgt
     3721 ccactatttg ctggtttgga ttgagaatgt gaacgattac aggatttttg tcttgggtac
     3781 tatttggtaa ggtataatct cacttgaaag tctgctcagc cccaaaagtt agaattcatt
     3841 ttagactttt atttgtagct ttcccctctt ttatatattg caaagtgtat tttgacagat
     3901 caacctgtgt tccagtaagt agtaataatg taatagtttc gttatcttta tcagatcaag
     3961 gtggatcata atcaatatgt tttatcttta tgtttttcaa agaagctata tttcaggtga
     4021 aaacttcttc ttccaactct tcatcaatct cttcttcatc tatatctatc cagaactcta
     4081 attggttttg gatattttct tcatctgttt cgctagcttt ggacatagag tttctacagt
     4141 atctgtctac aatctctcaa tcttttatca ggtttataca atggtcagtt tttgtaggga
     4201 atcagtaatt tcagacaaat gacatagtct gtttggcgaa caaagtattt cattttattg
     4261 attttatttc cccatcaaat cattgaatct tgtaatctct aagattaata tcggaattag
     4321 ttttattttt taatttcaaa atctgatagt ttcaataata tttcaagcta gatatttgta
     4381 tatctcaatt ggtggaatcg ttggtgttga attggaatca ttcagcttgc ggacagaatc
     4441 atcatcagtt atttacttta atatattgaa caactggctc tggagaaatc tgaataaatt
     4501 ggctttgtca tattgtcatt catggcgaaa acttgtctac ataatcaaaa actcctgcac
     4561 attgaatgaa atctgttcac atgtacaaag atgctcactg gattcatcaa tttagataaa
     4621 ccacatccaa cacctctaaa tcagccccat cttttcagta gacaatgagc catccagaac
     4681 tatttaaaga aaagtttgaa tcatataagg aatctcgtca ttcattatat cggacatcag
     4741 aatctgtgat gagaaacctc tttcctgctt gtaattcagt ggaactaaat actatttcct
     4801 gatccaaagc agatcctcaa agttttattt gagggtaatc atcccctaca tcatccgtaa
     4861 tattcagctc cacataagaa ggataaattc agcttcaata gtaaacttca gaaatatcca
     4921 aaatccaatc cgcataaaaa tcatcacaat ctatcggtat atttaaatca cttcccgtgt
     4981 tcccggggtc tggattgtaa ggatttttgt ctaatgtaac atcaatcaaa ttctctcaag
     5041 tggtgaaata cttagttgga tttgctgcaa ttcattgatt tatattgtag tatctgtcag
     5101 aatttaatcc aactactgtt gttgtccagc tatctcatgt tcaaattttt tcaaaagaag
     5161 ttttgtaatc ttgataataa tccacataac ttgaatggac aaaaaaatta tctagaacct
     5221 gtccagatcg gattaagtct aagttgaatt cttcatttgt gaaattgatg gaattaggta
     5281 ttgtctgaat gttttttcat gtctggaaca tcgaataaag agaatttgca aaaactttgg
     5341 atactcaaga tggaatattt gtagaaaatg tgaaattgag attcccagaa agactcaatt
     5401 ctccagagaa tgtatcattt caaatatttg tgatttcaaa ccggttttca tctcaatcaa
     5461 agtagatttc cgtagttttt aattgaggaa tattaggttc agttcaagaa tattcgttta
     5521 gatattctag tttaaaagaa ccaactcata agtttcatat tcatttttcg tatgtaagtg
     5581 ttatttgtgt gactgtgttt gaaacactaa atatgatatt tttttcttta tttggtaaga
     5641 aatccgcaaa ttcaaaaata acttcatcat tttgatctgt gaatattgct tttccggaaa
     5701 ataccgatcc tcaaggaaca gaactaccat ttgcttttgt tcgaacagtt gcttgtgcac
     5761 ctgttgtgag tatgaattct ggacttttta gccgatctca agaagaatca aattttaatt
     5821 taggggctgt tgatgtataa tcactaccta atcaataggc tgttcgtcat gttgggagat
     5881 tggctcttcc atccgcaaaa gaaacatcaa aaacaatatt accatttcat tctgtcgtca
     5941 attgagctac tagagggata aaattttctt cgccaaaaat ttcatcgttc ccaaaatcat
     6001 catccataca actctcattg ataatttcac catttgtcgt atcatcccaa aatcaagtct
     6061 gttcattttg tcattctcca acaaaatatt ctcaatttaa gtcttggttt tctaaattga
     6121 cacctacgaa taaattattg tctaaaagct gtccagtctg tcaacctttg gttatcggtc
     6181 accaaaaatc gtcgtctggc tctgtgtttt ctaagcttcc tttattttca aaaaaatcgt
     6241 cacttaagtt tgacgattct tgttgtggtt ccttatagtt ttctacctta tctcatcgaa
     6301 caaaactaga ggaaaaaaca gattgaaata aaattaaaat agacaataat acagacaaaa
     6361 tttttttcat ttcatcttat tagttgataa atttaatttt ttatattgtt ttgtctaaca
     6421 ttgtcaacat taaaaacatt gtgctgttaa ctgcgaacaa attataaacc accaactcaa
     6481 ataaacctaa tgtatttgta atatttgtaa atcaagtatt tggaaatcaa gctagatatc
     6541 tttataccaa aagaataact ctttttttta atcttctatt gactttttta tttctagatt
     6601 atccctcaat ctcctttctt ttactttaaa tcacccaact tattgtggat gtaaatacaa
     6661 attcaaatct acaaatacaa ggatttgtat tgaaacaatg aaataatgct tgatttaatg
     6721 aaaggatagg gttgttcttt atagttattt ctcaagttta tcagttcgtt tacccccata
     6781 gtattgtgaa gtggcacaga caatggagta gtgggaaaat gtaaaaatac taatccttat
     6841 aggactgaaa taacatatct tttatcttga aagtaataaa agttttatta ctatttgagt
     6901 agtttgattc tatatcaaaa cacatagatg gtttatttac tattgaaaat atgaaaaaaa
     6961 aacacatagc tatggcggga tgagggacat gatgacatgt ttttcccata aaaagtttaa
     7021 tagagcatat acacaaaaat ccaaaatatt ctcaaaaaat atcaaaaatc atccgatttg
     7081 ggcaaaaaaa atctctggaa aaagaacttt ttgatgaact aaaacaaaaa tatcctttac
     7141 tatattttca aaatatagtg tctggaaagt atcgcagaga aacttggttt agctctaaac
     7201 tgaaaaatgt tcgtgatatt ttcaaattta tgtttggaat atttcaatct atcttttatc
     7261 ttgttataca cagagttgat gtggtttttt gtaaatgatg atatgttgct ttaccagtag
     7321 tgtttgcagc aagattttta agaaaaaaaa ttgttgtcca tgaatcagat gttcgttctt
     7381 gactagttaa taaaatagca gcaagatatg caaacagagt ttttactggt tttgattgag
     7441 tttttgtaaa atcaacaaca gttggacaga tattgtcaga cgaaattatc agtaaacaaa
     7501 gtatcaaaaa tccaccaaaa gtaagagata tttcacaaaa aaatatagag ttgacgatac
     7561 aggaacagct tttttattct gatccatcca aaacctattt attagttatg tgatgatctc
     7621 aatgatccaa aagactttat caagacttac aaaatactat tgtctcaaat ccagaaattt
     7681 tcaaaaatta tgaaatattt gttgttttgt gaaaagaaaa tgttcaacaa aaagagctgt
     7741 ttgaaaaaat aaaaaatgtt catacttttg atttcctttc tcagtcggag atgtgacttt
     7801 tgctaaaaca ttgtgatatt tctctgactc gtgctggcac aacttctttg gctgaacaaa
     7861 aattatataa tctcaaaata atcatggttc caataccttg gactcacgac caatacgata
     7921 atgccaaata ttatgtaaaa cattacgatg atattttatt ggattcaaag gatccagact
     7981 acaaaaacaa tatgttgaaa atatttgacg attataagaa ttttaaaaaa agtgaacaaa
     8041 atgttgatat actttcggtg atttccaaag cgaaagatgt tattgtaaac gaacttataa
     8101 gttaaaaatc tctaaaaata ttttctattt ttgatttaga atattcttca tcatactttt
     8161 ttaaataagg ttcttttttt gttatttctt tatcaaatca tacggaaatt tttgaatcat
     8221 ttctcaattc ttccaccaaa tctcactcca atacaggatc gacttttcat aaatccctat
     8281 ctaaaataat gtcttgctca acttttgatt taagattttc actttccttt ttggctaaaa
     8341 aataatcata atctatttgc ttatgaatcc aatatttttc tggactaccc aaaacttgag
     8401 ataataaaat atcccactgt atagtgatgt ttctttttca ttttattaac tcgtttaact
     8461 cactaccact ttttcataac attaatgaaa aatctttttg agttaaacct cttgccctaa
     8521 tttcttctat taaaaattct ccaggatgtt tcatttttta tttgcttatt tcataaattc
     8581 tatgaactag tgaaattata ctcaaaaaga taaaatctgt caaatttttg aacacttttt
     8641 atacaattct atgaaacagt gaaatatata aaaaaaaata acaaggattt ggaataagaa
     8701 aatggatcaa taattatatt ttaaaaattt ttcactaatt ctacgaatta gtgaaaaaat
     8761 tatataaagt agtgaaaaaa tattttacat cgttttgaaa ttttaaaatc aaaaaaataa
     8821 ggtttgatta aacccaactt tttgtaagac cggtccaaaa aaagagtttt aaaaaattaa
     8881 ggagtttttt ttatattcat tcatatacaa aaaagttgta atttttctga tcaagatttt
     8941 ttgatataaa ttttttctcc aactattgga aatcatatac tagacaaatg tgcacgaatt
     9001 tggtgtctaa ctcattttga aattttaacc aacaatgttg tcgtattggt atttttgtta
     9061 taatacaatt tttctacatt tgtgtcaact tgatgggtcc tttttttgtc aattttccca
     9121 atttgcttcc tatctaaaaa tacaaccata cgatcctttg aaaatttgtg atgtcatatt
     9181 agatacgaaa tttttaggct attgggggca aattttcagt agacatctgc gacataatat
     9241 tttgttattt ttcatttaaa ctgtgcttgt ttatattttt cttttactag aggagttttt
     9301 gcgaagtata gcaatcatgc tgtatcatta tctaacctgt ttaataatca atattcttgt
     9361 tcttttccaa acatatcgtc taaataaaat aatattgatt tgatgtactg atcttttgat
     9421 tctaataaaa aatctaaaaa acatttttct tttcaaaaag tgcttggaat actgtgaggt
     9481 ttccaaaaat aataaaaaaa actatcttca taaactgtta ttttcatcaa ctctctccat
     9541 caggaagcgt aaagttgtaa gattaataaa gtgagaaagt ttgtaaagtt tataacttgt
     9601 aacttgataa acttgtaact ttcaacttgt aacttgataa acttattact ttatgaacta
     9661 actagaattt tcctttttct tttttatatt tacccaaaag tatataaacc aaggaggtag
     9721 ctataaatat tgaagcgaag gtagctgata taattccaac tcatatagta aacgcgaaat
     9781 gtttcattat ccctgttcca aaaacaaaca tcgctattac tattaataat gtagaaaaag
     9841 atgttcacac agatcttttc atagtttgcc aaatactgtc ttcaaacact cttcaataaa
     9901 tgacatcttt tcatgttcat ttattttgta aattttccct aaccctatca aaaattatta
     9961 tcgtatcgtt gattcaatat cccataatag tcaaaatagc gataataaat attgagtcta
    10021 tttgtattgt gttattaagc cacatccaca ccccataagc tcatgctggt atcgcaacat
    10081 caaaaatcat ggtaatagca acaaccagtc ataatattga tggagataca tagtttctga
    10141 tagctgaaaa agaaaacaac atataaatta caataaatat tactcatact ataagtgctg
    10201 tgatagctga cttttgcata taagagccaa cacttggtca tgtgattgtt tgttctatta
    10261 tctcatctgt agatgaaatt aaattatttt ccaataaata ttgttttatt tcatctgaca
    10321 attctgctac cttttcatct gtatctactt ttgtatttat tttgaatgtt gtagtatttt
    10381 ctattgtttc caaagaaacc cttaaatctt tatactgatg gcccaacaaa tactgtttta
    10441 gactttcctg ttctttttga ttgtctagta ttttgtctat tcataaactt attcatccag
    10501 taaactcttc tgaatatcta gcattagaaa agaagaaaaa aattgaagct aaaaataaaa
    10561 taatagaaat cgctatccaa atgggagctt ttttgataat attaaacctt gctttcatgt
    10621 taaaattata aaatttaaaa tttgttatcg taatatttaa gtatttatca aaaaaattca
    10681 atgggaaaaa gtattaagtg ggaagtccat caagttaaaa gtttgtaaag ttacaagtcg
    10741 atgactattc ctcttaaaaa agagggctaa aactttattt aaaaaattct tcccttgggg
    10801 gaagctgttc tgatgtagtt gggggtatta cccccaccta gtccctcata aattcgggac
    10861 accacaacct ccccaagggg agggatttta tcttaagtct tatgcacttt gaacttttaa
    10921 ctttaagaac ttattttaag cttttataga ttcataaaaa ttttgggcag catcttcttg
    10981 tgcttttttc ttattagtac caaatcattc tgcttttttg actcatagga catatatttc
    11041 cgccttgtaa gttaaaacat ttcatttaga gtcaatctca tgctcaatat ctttgtaaga
    11101 tggtaattct ttgtataatt tttgaaggtg ctcttgtacc atagttttgt aactttgtat
    11161 gggttttttg cttattttgt tatacattgt atatacattt ttttctacaa aaacttctgc
    11221 ttgagcgtaa ccaaagtcca aaaacacgaa tcatatcaaa gcttccaaag cgtctgccaa
    11281 aatagcgtct ttttttcttc attgcgtttt ttcttctcat ctactcacaa gaatatgttt
    11341 gtctaaatta atatttctag caacctgtgc caagtttcct tctctaacta atgctatctt
    11401 atataaagtc atctcagatt cgtccatctt aggatgtttt tggaataaca atttacatac
    11461 aatcgctcac aaaactcaat ctcacacaaa ttctaatctt tcattatgtc ataatatttt
    11521 tttgaaatca gcagcaaaag atttatgcac gaaagcttgt aataataaac cttcgttttt
    11581 tatttttgat ggatccaatc atagttcttt tatgtacgaa tatatttttt ctttttgttc
    11641 tagtactttt ttattaagca ttattgttgc aaaaaaatta aaaaattatc tttcgtaact
    11701 aaacggcgtg ttggctgtat aagaaataaa atcgtttcaa ataattaact ccatgtcata
    11761 attgagttct tctcatgtaa taaattgatt ttctcatttt tcaacgacat ttactgggaa
    11821 aaaatattgt aatgtgtcta tcgtctttga ataattttga tctcagtatg tgacaacttt
    11881 tgtttgttct atcgttcaag aatgattttc aattcatgca agattaaatc agtatttttt
    11941 taattttact gctattttat tggctcgtcc cgttggggaa agtctgtgtt gtgaagcaaa
    12001 gtttttgtct atagcatttt ttattaaaat tcttggattt tcgactagat atcaattatt
    12061 gtgggttaca aaaaaagtaa atctttgaat ataatcataa aaagaaatat tgttggattt
    12121 tgctccgttt ggtaccataa ctgcttgtcc accaaaagcc tctctgttcc cattgtaaag
    12181 aaaacatcca gcatctgtga gtttgtaaga agaataagta caatatgtgt ttaatcaaaa
    12241 actaaaaata tgtttaaaat cgtaaatata tctgaacatt cccaccattt cttttgaaga
    12301 tatatttgta taaaccatcc tagtataggt agaatacatt tcttttagtt ttcaaacatt
    12361 tgttatgttt tttctctgta aggctgtatc aatcaccgct tttatcacat tttgctgtct
    12421 ttgagatctg gcaaaatctg atgtagtgtg tctagatcta acatatttga gtgctgtatc
    12481 tcaatctaaa gtttgttgtc atgcagagat atggaatgtt tgatatcata aattatcatc
    12541 tgggtatgta atatcgtgta ttgtgttttc gatataaata tctattcatc ataaagtgtc
    12601 cactatttct ttgaatcatt gaaaatccgc tactagataa tatggaattt ctagtcacaa
    12661 aatttcttct acttttgcaa taagattttc tgctccagat catatattat ttcatccgcg
    12721 actatatcca cgagcaaaca aaccgtttat tcttcataaa tatcaagaag aattgacata
    12781 caagtctctt ggaatagata tcatcgtaac ggctcataat tccggatttc gtgaggttgc
    12841 tatcatagaa tctgccaaat atcatcaatg gtgatcatct catcaaattc aaaccaacat
    12901 aatatttaca ttaccaaact catctctacg catttcttgt caaagattgg aactaactgt
    12961 tttaacggta gactttcaca atgagcccaa aagcacatgt ccaccatgaa ttaagttttt
    13021 tcaaaaaaat attatcagag acaaacatat taaaaaaacg atgtatttta atatttttga
    13081 aaaaattttg ttttgtttgg aatctgtttc atgtatttct catacttttg attgtttaaa
    13141 ctttgctcac ataagttaac tattatttat ttataaaaat atcccccgta gataatatat
    13201 gattcaaaga aaagctatct cacaatattt gctttaacat aatatatgaa tctggttttt
    13261 tgaaaacaaa tcaaaaatct tctattattt tttgttcggc ctttatgttt atttttatgt
    13321 ttttttcaag atctgttcaa taaatgaatt tattggaaac ctctatgttt ccatttataa
    13381 tatttttaat aaaattttct gactttttat ttattatatt tatatttaaa cttatcatat
    13441 cttgataatt agctaattgt tttagctgaa acttacattg gcttttatga aaacttaaag
    13501 aaactgcata agttgttcat tccttttctg gaatttttat gactaaatcg ctatttttgt
    13561 cgtcaatgtt tcattttata gtgtagtttt tgtcattata ataaagttct aagttttcta
    13621 tttttaattc tatgtctgag tcagatttaa taatcaaaag tcattcaaaa aaattaattt
    13681 taaaatttgt gttatctgaa atgtaatcaa caacatcttg ctcaattttt attttatatg
    13741 caaaaaatca attatattta agctcattta ttggataaaa aatatttgat gctaaaatcg
    13801 tttgtaataa aaaactaaaa tctttgtaaa aatttgtatt acttttttcc tttaaatcta
    13861 agaacattcg tttgttttct agttttttag aaattagtaa caatagattg ttttgatagt
    13921 tccctgagcc taaatctata aaaaaatcct gcaaattaat atatctttca ttctctatgt
    13981 ttttgtaagc tatattccct gaaagattta atatgttttt ttctttttta tctaaaaagt
    14041 gtagtgagat ttggttgtgt aaatcttgtt tgttagctaa ataatttgta taggcattta
    14101 attctatgtt tgagttgagt agaaaatcag cattttctca gtctatttcc aacttagttt
    14161 gagtttcttt ttgtcagcta aatcataaat cattgatact gtttaagata taactaattt
    14221 gttgtgtaaa aacagagata gcttgattaa aatccatctc caagtacata tttttttgac
    14281 aagcagacaa aattatgact ggaaaaagaa gtattataaa atattttttc atattattta
    14341 tgaaattatt gttaaatgtg gtttgtttat atttaatttt tgattacttt caacagtaaa
    14401 gatttgaagg agcaaagaaa gtaaaattta ttgcctccct tcaaagaagg gagctgctct
    14461 aatgctatgc accgcttgcc ccgcttaaac agcggggcag aattgcgggg ctacaattta
    14521 tggggattta ggccacaaat agaaaaaaca aaaaattgtc tcccttttgc gaaacgaagt
    14581 ggagtcctct cccgacacat cgggatctca ctttgttcga gatctatcgg gacaaaggga
    14641 gctgttcttc cgcatctctt gcaggcgcga attcgaattc gcgcttataa gaggtggaag
    14701 ttaagccata agtaggagaa acagcaaact gcctcccttt cgcaaaggga gctggtctcc
    14761 ccgcaccgta gtactacggt gcggggtaga gggatttatt tacacccctg tacaagttac
    14821 ttaattcggt aacaaatttt ttgtaaaatt aaaaggagat
//
LOCUS       DAOSID010000012        13913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_4485_length_13913_cov_13.810579, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000012 DAOSID010000000
VERSION     DAOSID010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 13913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13913
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4485_length_13913_cov_13.810579"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1456)
                     /locus_tag="PK674_01325"
     CDS             complement(116..1456)
                     /locus_tag="PK674_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15207.1"
                     /translation="MTNYVKNIKESTLKNKFNLPNNFDIKNFNIAIERSFHEFLKSKD
                     FKKLDNNTKIKDIQNKFNERLDRNLNKLYKIKKLKMDNTRKFNFQLQNFNTKFCLKTL
                     TKKTKGNPSLEEGDLDIKTEEHLNKIFKKIENSKDFDPNSEKVLKDYIEKGIGFGVIK
                     EVFNILWRFIKGGNNVGFSEFFNLSTDIKKLDLDNKSINELDSMINKFETQIEKTLDI
                     KKDLKLTYILSEIKNKKLLKKNPSLNKKRLLQKNLKIGDVVLLNKKADKLNIGGKAFK
                     AHIGSKALEAYDKKYDTDFTHSIIIIGTDPIKIRHSTMLTQKDDKGGHVEEVDLYSYL
                     EKSGTKSFDVLALRSDEDTKQKILEFSEKKLGKKYDNNAALGGGLFGIDSMGAKTRGG
                     FIKNKTLKKDDVYNCVEIIAQALEHDKLDNITHPNEFLEYMNIFQPTYMTTIVL"
     gene            complement(1574..2251)
                     /gene="ung"
                     /locus_tag="PK674_01330"
     CDS             complement(1574..2251)
                     /gene="ung"
                     /locus_tag="PK674_01330"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOG15208.1"
                     /translation="MINPQIEESWKKELWDEFQKPYFSEIKNFLQKEKSEGKTIYPEG
                     KNIFNAYNSTPFDKVKVVLLGQDPYHQPGQAHGLCFSVKPGVRFPPSLQNIFKELETD
                     IGIKIPTNGYLQKRAEQGVFMLNASLTVRRGEPMSHSQIGREKFTDATIKTLSDKRNG
                     LIFVLRGSFAGSKKTLIDTNRHHVLEAPHPSPLSSHRGFFGCKHFSKINEILKNNGEK
                     EIDWQIE"
     gene            complement(2275..2346)
                     /locus_tag="PK674_01335"
     tRNA            complement(2275..2346)
                     /locus_tag="PK674_01335"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2312..2314),aa:Arg,seq:ccg)
     gene            complement(2456..5560)
                     /locus_tag="PK674_01340"
     CDS             complement(2456..5560)
                     /locus_tag="PK674_01340"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOG15209.1"
                     /translation="MNESTIENLLIQLLQEQGYTYQHGSSTIPQADPQLRTARDEVIL
                     SPIFKKQLKIINPSASESALKEVYQKLTHLDASDLLSKNEQFHQWLRDGIKIESFHDG
                     ETENEHIFLVDQEHLERNDFRVINQFTVKENNLEKRCDVVIFINGMPLVLIELKNPLD
                     ENADLYRAYTQIQNYQTAIPSLFQYNGLCVISDGIEARVAPFSAPFSRYLAWKEPVGS
                     QKGGIITELEVLTKNLLDPATLLDVVINYTVFENEEQKDEETGQIFIDKLKKTAAYHQ
                     YYAVEKAIASTLQAVNGDRKIGVVRHTQGSGKSLSMVFYAGKLITHPELKNPTLLVIT
                     DRNDLDDQLFGTFSNARGLLRQTPQQAESREHLKSLLKVAGGGIVFSTIQKFFPEEGN
                     VFDTLSKRENVIVIADEAHRSQYGFKAKAVLNEKTKESELRYGNAKYLRDALPNASFI
                     GFTGTPIERTDKSTRGVFGDDIDVYDIKQAVEDGATVPISYESRLLKIGIDQSVLEEI
                     DASIEEFNNVNPSDKKAIQSKLAKIENLIGNPEILELLAKDFVMHFEQRQEVFEGKAM
                     VVAMSRKILVRIYDQIVKLRPAWEKQGKIKVIMTSSSDDPAEFQPHFTTSKERKALAI
                     AFKNPEDPFQIALVCDMWLTGFDVPCLHTMYLSKRMQGAALMQAIARVNRVYKDKAGG
                     LIVDYIGIAQDLRDAMSVYTQSGGTGLAVHEKEQNIAELFTKYEVVEQMFHGYEWKKY
                     FSASPQEKLKILIGACDWIVQSKDLKQRFLKETLILNKLFTLAVPSPEADQLRDAVAF
                     FQAIKTRIMKISRSRVENIKLETTIKQILDSAVFHGEVIDIFEAIGMEKPNVDLLSDD
                     FLLEVKNMKHKNLALELLKRILNDEIFLRQRTNIAQAKKFSELINGVLKKYTNAQIDS
                     AQVIQELCDIAKALHLEDQSAQELGLTAEEFAFYTVLSQNESTKLLEDEKMKELIHVI
                     VARVRKSATGLWLKKSDIQAKLRVEVKKILQQYGYPPDLARMEADKVLEQSELLAEQM
                     ARQG"
     gene            complement(5590..6495)
                     /locus_tag="PK674_01345"
     CDS             complement(5590..6495)
                     /locus_tag="PK674_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF6430 domain-containing protein"
                     /protein_id="HOG15210.1"
                     /translation="MKRQFKSLLSKRKDIGKDFLSILGIIRLIFEILLYFFAELEKHK
                     SIKIFIVMIFMSIVISFYRNRPKKYCKRKLRDIDSWIEIKIGDIFKNEGAIIVPINDY
                     LDLKLGGNVKRSNSIQNRMIKDYFGKVEYLESELSEYSDKLPLDIGTIIDIGDMGFPN
                     KKHFYLLINSKKQKNNHVSCNTDDFMVSINKLWDYIADNGNKGEIISVPLISAQHNKD
                     SNLTRSVIIKQLIDSFIEAAKSKSIIENVIISIYPDDLTKGDINFQEICDYLTFQTKN
                     YKKIEYDTKSVGTPYTPSTIKKISY"
     gene            complement(6497..7000)
                     /locus_tag="PK674_01350"
     CDS             complement(6497..7000)
                     /locus_tag="PK674_01350"
                     /inference="COORDINATES: protein motif:HMM:NF020509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="TIR domain-containing protein"
                     /protein_id="HOG15211.1"
                     /translation="MVYANKTYIAFDADNDIHYYRLMCARKHNDNTSFNFYDAHDLNN
                     LRSYASEEQIKRKLSERMQNAKIFILLVGENTKNLFKFVRREIEQAIKKGLPIIVVNL
                     NGKRSMDTNLCPAILKDELAIHISYNQKIIEYAMNNWINSFYELKQEGNTGPYYYKQN
                     IYQNLGL"
     gene            complement(7121..7327)
                     /locus_tag="PK674_01355"
     CDS             complement(7121..7327)
                     /locus_tag="PK674_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15212.1"
                     /translation="MNLEKNGHLCSSYPKNFAVSVPNPIKMEEGAPAAKTPPKPVEKG
                     TSTAKTPPKPVEKGTSTTKTPPKK"
     gene            complement(7318..7791)
                     /locus_tag="PK674_01360"
     CDS             complement(7318..7791)
                     /locus_tag="PK674_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15213.1"
                     /translation="MSEKKQSEQMAISERQEFVQIRQHYIDSAHRSSNVFDSTMITLT
                     TAAIGVSLFMLDKKMSDVSFLYFLIRSRVLLGVTLLLILFSFIISEKAHRLSIDLHDM
                     QYIKNDSNKKQEEKIECKIKTMNQILSLMKHIQIITLVCGIVFLVLFLLKNIKGI"
     gene            complement(7811..9049)
                     /locus_tag="PK674_01365"
     CDS             complement(7811..9049)
                     /locus_tag="PK674_01365"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOG15214.1"
                     /translation="MIKNSKAMTKVPEGWKETTLGEVSEIKGGKRLPKGDILVSYKTQ
                     HPYIRITDFENHKIKKDQLQYVSDEVYKSISRYIVNTGDVILSIVGSIGLVAYIDEYL
                     NKANLTENCVKIIRLRSVNPLFLYYFLISNLGQNEINKNTVGAVQKKLPIYGVQNIKL
                     TLPPLPEQQAIAKVLSSFDDKIELLREENQTLEQMGQELFKEWFGKWKVDDDLPEGRR
                     VGKIEDIFHFLEGPGIRNWQYTDSGTKFINIKLIQNQDILTNNANFVSNEEAYGRYKH
                     FLLEERDFVLSTSGTLGRSAIIRRKHLPLMLNTSVIRFRPLDKKSYGFMYLYLSSRAF
                     IHQLESMASGSVQLNFGPMHLKQIDLIIPDNLIIDQFCKITNNLFEKITKNLDQIDEL
                     SATRDQLLPKLMSGEVRVEF"
     gene            complement(9199..10263)
                     /locus_tag="PK674_01370"
     CDS             complement(9199..10263)
                     /locus_tag="PK674_01370"
                     /inference="COORDINATES: protein motif:HMM:NF025157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="Fic family protein"
                     /protein_id="HOG15215.1"
                     /translation="MSNYVIPKLPLDIDVETKRILKKVSTARAALAELNGTTKLIPNP
                     TILINSLTLQEAKDSSAIENIITTHDELYKADIKIGNITLQTKEVQNYRQALLFGYEL
                     VKKHDCILINDIVKIQEKLEHNTVGIRKIEGTVLMNDKTNDIVYEPPQNYNDIVNYLT
                     NLENFINSDDDVDPLIKMAIIHHQFESIHPFHDGNGRTGRIINVLYLILKKLLDLPVL
                     YLSGYIIANKSEYYHQIQEVRDSGNWEDRIYFILDGVEETALMTTNTIKNIWDIMGET
                     KKLMKSQLSFYSKDFLELLFKHPYTKIDFVVKELGVVRQTASKYLQELENLGIVEMVQ
                     IQKEKYYINVKLYDLLRKGF"
     gene            complement(10274..11791)
                     /locus_tag="PK674_01375"
     CDS             complement(10274..11791)
                     /locus_tag="PK674_01375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOG15216.1"
                     /translation="MAKEQNGNFEAQLFKAADKLRKNIDAAEYKNVVLGLIFLKYISD
                     SFQELYDQLANKAFSPYDDPEDRDEYTAKNVFFVPQQARWNHLQARAKLPTIGIELDQ
                     AMDAIEKENPQLKNVLPKVFGKPNLDVMSLGGLIDLISNTELKEQSQSSKDLFGRVYE
                     YFLGEFANAEGKKGGQFYTPKAIVKLMVEMIEPYKGLVYDPACGSGGMFVMSEKFVKE
                     HQGNVQDITIYGQESNQTTWKLSKMNLAIRHINSEFVAWNTEGSFLKDAHPDLKADFV
                     LANPPFNQSDWGQELLQGDARWQYGLPPAGNANFAWMQHMLYHLKPTGVMATVLANGS
                     LSSNTGGEGEIRKNLIQNGLVECIVALPKQLFYNTGIPACIWFLRRGRKENSDKILFI
                     DASELGFMKTRVHRDLSDEDIARIANTYHNRRKGEHYEDQLGFCKSANLDEIEAQGFV
                     LTPGRYVGIPEEEDDGIPFEEKMKNLTSTLSEQMKQEQVLNEEIKNQLGKIGFSL"
     gene            complement(12156..12228)
                     /locus_tag="PK674_01380"
     tRNA            complement(12156..12228)
                     /locus_tag="PK674_01380"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12193..12195),aa:Trp,seq:cca)
     gene            complement(12247..12636)
                     /gene="rnpB"
                     /locus_tag="PK674_01385"
     ncRNA           complement(12247..12636)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK674_01385"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(12766..13218)
                     /locus_tag="PK674_01390"
     CDS             complement(12766..13218)
                     /locus_tag="PK674_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="NYN domain-containing protein"
                     /protein_id="HOG15217.1"
                     /translation="MGTDVILGKFNRVEKTFKNGKNVLEEIQPKDNDVPDLLKFITYE
                     EKETDVNIALKIFEDGVFDNYDKAIIVSGDSDIIPAIRRVRDLSKKGKIGNKEFVSLL
                     FPRSKGKMIVKTCSSRLNIDSKHIENSLLPNKIDIGNGKHIIKPKSWK"
BASE COUNT         4291 a         2176 c         1907 g         5539 t
ORIGIN      
        1 tagaaaaggt tagagaatga gcagaatggt tattggaaca ttatccatga cttgagtgac
       61 tggatgcatt aaaaaaatac ctagatgaaa aaataattaa ggttttggtt tagttttata
      121 gtacaatagt tgtcatatat gttggttgaa aaatattcat atattccaaa aactcatttg
      181 gatgtgttat gttatccaac ttatcatgct ctaaagcttg tgcaataatt tctacacaat
      241 tatacacatc atcttttttt aaagtcttat tttttataaa tcatcatcga gtttttgctc
      301 acattgaatc tattcaaaac aatcatcatc ataatgctgc attattatca tatttttttc
      361 ataatttttt ttcactaaat tctaatattt tttgttttgt gtcttcatca gatcttagag
      421 ccagtacatc gaaagatttg gttcaagatt tttctaaata actatataaa tcaacttctt
      481 ctacatgtca tcacttatca tctttttgag taagcatagt tgaatgtcta atttttattg
      541 gatcggttca tattattatt atgctatgcg tgaaatctgt gtcatatttt ttatcatatg
      601 cttctagtgc tttacttcat atgtgtgctt taaatgcctt gcctcatata tttaatttat
      661 ctgccttttt gtttaataaa acaacatctc ctatttttaa atttttttgc aataatcttt
      721 ttttgtttag acttggattt tttttaagta attttttatt tttgatttca cttaatatgt
      781 atgttaattt taaatctttt tttatgtcta atgttttttc tatttgtgtt tcaaatttat
      841 ttatcataga atctaattca ttaatacttt tattatctaa atctaatttt ttaatatctg
      901 ttgataaatt aaaaaattct gaaaatcata cattattacc gcctttaatg aatctccata
      961 agatattgaa tacttctttt attactcaaa atcatattca tttttctata taatctttta
     1021 aaaccttttc tgaatttgga tcaaaatctt tactattttc tattttttta aatattttat
     1081 ttaaatgttc ttcagttttg atatctaaat ccccttcttc taaactcgga tttcattttg
     1141 ttttttttgt taaagttttt aaacaaaatt ttgtattaaa attttgtaat tggaaattaa
     1201 attttcttgt attatccatt tttaattttt taattttata taatttgttt agatttctat
     1261 ctaatcgttc attaaatttg ttttgtatat cttttatttt tgtattatta tctaattttt
     1321 taaagtcctt agacttcaag aattcatgaa aagacctttc gattgctatg ttgaaatttt
     1381 taatatcaaa attatttggt aaattaaatt tattttttaa tgttgattct tttatgtttt
     1441 ttacgtaatt tgtcattttt tattaattat aatttaataa ctagaataag tataattaaa
     1501 aataaaaaat tgtcaaaata tgttattttt caactatttg ttgtttgaaa aagataatgt
     1561 gtttggtaat aaattattct atttgccaat ctatttcttt ctctcaatta ttcttgagta
     1621 tttcatttat tttagaaaaa tgtttacatc aaaaaaaacc cctatgagag cttaatggag
     1681 atggatgtgg tgcttctaaa acatggtgtc tatttgtgtc tattagtgtc ttttttgatc
     1741 aggcgaaaga tcatcgtaaa acaaatatta atccgtttct tttatctgat aatgttttta
     1801 ttgttgcatc tgtaaatttt tctcgtcata tttgtgaatg agacattggt tctccccttc
     1861 taacagttag tgaagcgttg agcataaaaa ctcactgctc tgctcgtttt tgtaaatatc
     1921 agtttgttgg tattttgatt catatatctg tttctaattc tttgaatatg ttctgtaatg
     1981 atgggggaaa tctcactcat ggtttaacgg aaaaacatag tccatgtgct tgtcatggtt
     2041 gatgataggg atcttgtcat agtaaaacta cttttacttt gtcaaacgga gtgctattgt
     2101 atgcattaaa tatatttttt cattctggat atatggtttt tcactctgat ttttcttttt
     2161 gtagaaaatt ttttatttct gaaaaatatg gtttttgaaa ttcatcccat agttcttttt
     2221 tccaagattc ttctatttga ggattaatca tattttgagt tttttataaa aaatcaaggt
     2281 cggtaggaat tgaacctact accaatggct tcggagacca ctgctctacc ggtgagctac
     2341 gaccttttct atttttataa taataaaatg attatagtaa tcaaacagat tttttcaaaa
     2401 tcactaaaaa ataagaatta aaattatttg aaaaatatga taaaaaatag acattttatc
     2461 cttgacgagc catctgctcc gctagaagtt cgctttgttc aagcacctta tctgcttcca
     2521 ttcttgcaag atcaggtgga tagccatatt gttgaagaat ttttttcact tctactctta
     2581 attttgcctg aatatcggat tttttgagcc aaagtcaggt tgcactttta cggacacgag
     2641 ctacaatgac atggatcagt tctttcattt tttcatcttc caatagtttt gtggactcgt
     2701 tttgggaaag aacggtataa aaagcaaatt cctctgctgt caatccaagc tcttgtgctg
     2761 attgatcttc caaatgtaag gctttggcga tatcacagag ctcttgaata acttgagcgc
     2821 tatcaatttg agcatttgta tattttttca aaactcagtt tatcagttct gaaaactttt
     2881 ttgcttgagc aatatttgtt ctttgtctta aaaaaatttc atcgttcaaa attcttttta
     2941 gaagttcaag agctaggttt ttatgtttca tattttttac ttccaataag aaatcatcag
     3001 aaagtaaatc aacattcggc ttttccattc ctattgcttc aaaaatatct atcacttctc
     3061 aatggaaaac tgcagaatct aaaatttgtt taatggttgt ttctagttta atattctcta
     3121 cacgagatct ggaaatcttc ataattctgg tttttattgc ttgaaaaaaa gcaacagcat
     3181 ctctgagttg atcggcttct ggagaaggga ctgctaaagt gaaaagttta tttaagatca
     3241 atgtttcttt caaaaatctt tgtttgagat ctttagattg tacaatccaa tcacaagctc
     3301 cgatcagaat ttttaatttt tcttgagggg aggctgaaaa atattttttc cattcgtaac
     3361 catgaaacat ctgctctacc acttcatatt ttgtgaaaag ttcagctata ttttgttctt
     3421 tttcatggac tgcaagtcct gttcctccac tttgagtata tactgacata gcatctctaa
     3481 gatcttgagc aattcctata taatcaacaa tcaaacctcc tgctttatcc ttataaacac
     3541 gatttactct agcaattgct tgcattaaag ctgctcattg catcctttta cttaaataca
     3601 ttgtatgtaa acaaggaaca tcaaatccag taagccacat atcacaaact aaagcaatct
     3661 gaaaaggatc ttcaggattc ttgaaagcta tagcaagagc ttttctctct ttggatgtgg
     3721 taaaatgtgg ttggaactct gcggggtcat cactagaaga agtcataatt actttgattt
     3781 tgccttgttt ttcccaagcg ggacgaagtt ttacaatttg atcatagata cgaactaata
     3841 tttttctact cattgccact accattgctt tcccttcaaa aacttcttgt ctttgttcaa
     3901 aatgcattac aaaatcttta gctaaaagtt ctaatatttc tggatttcca attagatttt
     3961 caattttggc aagtttagat tgtatagctt ttttatcaga tggatttaca ttgttaaatt
     4021 cttcaatact agcatcaatt tcttctaaaa ctgactgatc aattcagatt tttagtaatc
     4081 tactttcata actaatagga acggttgctc catcttctac agcctgttta atatcgtaga
     4141 catcaatatc atcaccaaat actcatctgg tagatttatc tgttctctca ataggtgtcc
     4201 ctgtaaagcc aatgaatgaa gcatttggca aagcatctct aagatattta gcattcccat
     4261 atctcaattc tgattctttt gttttttcat ttaatactgc ttttgctttg aaaccatact
     4321 gactacgatg cgcctcatca gcaataacaa taacattttc tctttttgat aaagtatcaa
     4381 aaacatttcc ctcttctggg aaaaattttt ggatagtaga gaaaactatt cctcatcaag
     4441 ctactttcaa cagacttttg agatgttcac gagattctgc ttgttgaggt gtttgtctaa
     4501 gtaatcaacg agcatttgaa aaagttccaa aaagttgatc atcaagatca tttctatcag
     4561 taattaccaa taaagttgga tttttgagtt cgggatgagt gataagtttc cctgcataaa
     4621 aaaccataga aagcgatttt cagcttcctt gagtatgtcg cacaactccg attttgcggt
     4681 ctccattgac tgcttggagt gtggaagcta ttgctttttc aacagcataa tattgatgat
     4741 aagcggcggt ttttttgagt ttatcaataa aaatctgtcc agtttcttca tctttttgct
     4801 cttcgttttc aaaaacagta taattaataa ctacatcaag taaagtagct ggatcaagta
     4861 agttttttgt aagaacctct aattcagtaa ttattcatca tttttgagaa ccaactggct
     4921 ctttccaagc aaggtagcga gaaaaaggag cagaaaaagg agcaactcta gcttctatcc
     4981 catcagaaat cacacatagt ccgttatatt gaaagagaga aggaattgcg gtttgataat
     5041 tttggatttg agtataagct ctatagagat cagcattttc atcaagtgga tttttgagtt
     5101 cgataaggac gagtggcatc ccattgataa atatcacgac atcacatctt ttttctagat
     5161 tattttcttt gacagtaaac tgattgatca cacggaaatc gtttctttct agatgttctt
     5221 gatctacaag gaagatgtgt tcgttttcag tttctccatc gtgaaaagat tctatcttga
     5281 ttccatctct gagccattga tggaattgtt cattcttgga aagcagatct gaagcgtcta
     5341 aatgagtgag tttttggtag acttctttca aggcagattc agaagcgctt ggattgatga
     5401 tttttagttg ttttttgaaa attggagaaa gaatgacttc atctcgggca gttctcaatt
     5461 gaggatcggc ttgaggaatg gtagatgagc cgtgttgata agtgtagcct tgctcttgga
     5521 gaagctggat aaggagattt tctattgtag attcgttcat taaggctcat aagcaggtaa
     5581 atatttattt taatatgaaa ttttttttat tgttgatgga gtgtatgggg ttcatactga
     5641 ctttgtatca tattcaattt ttttataatt tttagtttga aaagttaagt aatcacatat
     5701 ctcttgaaaa tttatatctc atttagttaa atcgtctgga tatatagata taataacatt
     5761 ttcgattata cttttacttt ttgctgcttc tataaatgag tcaataagtt gttttattat
     5821 aacacttctg gttaagttgg aatctttatt atgctgcgct gagattaatg gcacagaaat
     5881 aatctctcac ttatttcaat tatcagcaat gtaatcccat aatttattta tactgaccat
     5941 aaaatcatct gtattacaag aaacatgatt atttttttgt tttttagaat taattaacaa
     6001 gtaaaaatgt tttttattgg ggaatcacat atctcagata tcaattatag ttcaaatatc
     6061 tagtggtagc ttatctgaat attccgataa ttcagattct aagtattcta cttttcaaaa
     6121 gtaatctttt atcatcctat tttgtatact atttgatctt tttacgttac ctcataattt
     6181 taaatctaaa tagtcattta ttggaacaat tatggctcat tcattcttaa atatatctcc
     6241 aatttttatt tcaatccaag aatctatatc tcttaacttt cttttacaat attttttagg
     6301 ccgattacga taaaaagata taactatcga cataaaaatc attacgataa aaatttttat
     6361 acttttatgc ttttctaact ccgcaaaaaa atataaaaga atttcaaaaa taagtcggat
     6421 tataccaaga atagataaaa aatcttttca tatatctttc cgttttgata ataatgattt
     6481 aaattgtcgc ttcattttac aatcataaat tttgataaat attttgttta taataatatg
     6541 gtcaggtatt tcattcctgt tttaattcat aaaaagaatt tatccaatta ttcatagcat
     6601 attcaataat tttttgattg taactaatat gaatagctag ttcatctttt aatattgcag
     6661 gacataagtt agtgtccata cttcttttcc cattgagatt tacaacaata ataggtagtc
     6721 ccttctttat cgcttgttca atttctcggc gtacaaactt aaagaggttt tttgtgtttt
     6781 ctcagactaa gagtataaag attttggcat tttgcattct ttctgatagt tttcttttaa
     6841 tttgctcttc tgatgcataa cttctaagat tattcaaatc atgagcatca taaaaattga
     6901 atgatgtatt atcattgtgt tttcgagcac acattaaacg ataataatgt atgtcattat
     6961 ctgcatcaaa tgcaatatag gttttattgg catagaccat gataatatta tttaaaagat
     7021 aaaattgttt tttatttctg tttttgtttt tatattataa ttttttaaaa tcttataata
     7081 tactcttcta aatcaatcat atttaaaaaa acaaacctga ctatttcttt ggtggtgttt
     7141 tggtagtaga tgtacctttt tctacgggtt ttggtggtgt tttggcagta gatgtacctt
     7201 tttctacagg ttttggtggt gttttagcag caggcgcacc ttcttccatt tttattggat
     7261 taggtactga aactgcaaaa tttttaggat aagaactaca taagtgtcaa tttttttcta
     7321 aattcatttt atgtttttta ataaaaataa aactaaaaat actataccac atactaatgt
     7381 aattatttga atatgtttca ttaaactaag gatctggttc atagttttta ttttacattc
     7441 tatcttttct tcttgttttt tatttgaatc attctttata tattgcatat catgtaaatc
     7501 tatagacaat cgatgagctt tttcacttat tatgaaagaa aatagaatta ataacaatgt
     7561 tactcataat aatactcgac ttcttataag aaaatataaa aatgaaacat cgctcatttt
     7621 tttatctagc ataaatagag atactcatat tgcagcagtt gttaatgtaa tcatagtact
     7681 atcaaaaaca ttagaagatc tgtgtgctga atcaatataa tgttgtctaa tctgaacaaa
     7741 ttcttgcctt tcagatatag ccatttgttc agattgtttt ttttctgaca taacttttta
     7801 gtttatcaat ctaaaactcc accctcactt ctccactcat caactttggt aaaagctgat
     7861 cacgagtagc tgaaagttca tctatttgat ccaaattttt tgttattttt tcaaataaat
     7921 tgtttgtaat tttacaaaat tgatcaatta ttagattatc tggtataatt aaatctattt
     7981 gtttcaaatg catcggtcaa aagtttaatt gtacgcttca tgatgccatt gattctaact
     8041 gatgaataaa cgctcttgat gacaaatata gatacataaa tcaataagat ttcttatcta
     8101 gaggtctaaa tctaataaca ctcgtattta gcatcaaagg caaatgtttt cttctaatta
     8161 tagcacttct gcccaaagtt caagaagttg ataaaacaaa atctctttct tctaaaagaa
     8221 aatgtttata tcttcaataa gcttcttcat tagaaacaaa attagcatta ttggttaaaa
     8281 tatcttgatt ttgaattaat tttatattta taaactttgt tcatgaatca gtatattgcc
     8341 aatttctaat tcatggtcac tctaagaaat gaaaaatatc ttcaattttc ccaactctcc
     8401 gcccttcagg taaatcatca tctactttcc acttcccaaa ccactccttg aaaagctctt
     8461 gtcccatctg ctcaagagtc tgattttctt ctctgagaag ttcaatctta tcatcaaagg
     8521 aactaaggac ttttgcgatg gcttgctgct ctggaagtgg gggaagggtg agttttatat
     8581 tttgaacccc ataaattggt aatttttttt gcacagctca aaccgtattt ttatttatct
     8641 cattttgtcc taaatttgaa attaaaaaat aatataaaaa taatggattt acacttctta
     8701 aacgtataat tttaacacaa ttttctgtca aattagcttt attcaaatat tcatcaatat
     8761 aagcaacaag gccaatactt ccaacaattg aaagaattac atctcatgta tttacaatat
     8821 atctagatat gcttttgtat acctcatcac ttacgtattg taactgatct tttttaattt
     8881 tatgattctc aaagtctgta atacggatat aaggatgttg tgttttatat gaaacaagaa
     8941 tatctccttt aggcagcctt ttgcctcctt ttatttcaga aacttctccc aaagtcgttt
     9001 ctttccatca ttctggtact tttgtcattg ctttactatt tttaatcatt caaattgtta
     9061 taaattaaat ttgattttca attggtcaca atttaaagac acttcatgtt aatttttttt
     9121 gattttttta acatgacaca tctctcatgt taattttttt tgattttttt aacatgacag
     9181 atccctcatg ttaaatgttt agaatcactt tcttaaaaga tcgtacaatt taacattaat
     9241 gtaatatttt tctttttgta tttgtaccat ttcaacaatt cataggtttt ctaattcttg
     9301 caaatattta ctagcagttt gtctaacaac tcataattcc tttactacga aatctatttt
     9361 tgtataagga tgcttaaata acaattctaa aaaatcttta ctataaaagc ttaattgaga
     9421 tttcattaat ttttttgttt ctcacataat atcccaaata tttttgatag tatttgttgt
     9481 cattaatgct gtttcttcta ctcaatctaa tataaaatat atccgatctt cccaatttca
     9541 gctatctctt acttcttgaa tctgatgata atactctgat ttatttgcta taatatatcc
     9601 actgagatat aaaacaggta aatctaataa ttttttcaga attaaataaa gaacattgat
     9661 aattcttcaa gtacggccat tcccatcatg aaatggatga attgattcaa attgatggtg
     9721 aattattgcc atttttatca gtggatctac atcatcatct gaatttatga aattttctaa
     9781 attagttaag taatttacaa tatcattata attttggggt ggttcataca ctatatcatt
     9841 tgttttatca ttcattaaaa cagtaccttc tatttttcta attcaaactg tattatgttc
     9901 tagtttttct tgaattttga caatatcatt aatgagaata caatcatgtt tttttactaa
     9961 ttcataccca aataataaag cctgtctgta attttgtact tccttggttt gtaacgtaat
    10021 atttcatatt ttaatatctg ctttatagag ttcatcatga gtagttataa tattttcaat
    10081 agcagaacta tctttagctt cttgtaaagt taaggaattt atcaaaatcg tgggatttgg
    10141 aattaattta gttgtaccat ttaattctgc taaagccgct cttgctgtag aaactttttt
    10201 caaaattctt tttgtctcta catctatatc aagtggtaat tttggaatta cataattact
    10261 catcttaaat tttttataaa ctaaatcaaa tttttcataa ttgattcttt atttcttcat
    10321 tcaaaacttg ttcttgtttc atctgttcag atagagtaga agtcagattt ttcattttct
    10381 cttcaaaagg aataccatca tcttcctcct caggaatccc tacataacga ccaggagtca
    10441 aaacgaaacc ttgagcctca atctcatcaa gatttgcaga tttacagaat caaagctgat
    10501 cttcataatg ttctcccttt cttcgattat gataagtgtt tgcaatcctt gcaatatcct
    10561 catccgagag atctctatga actcttgttt tcataaaccc taattcagaa gcatcaataa
    10621 aaagtatttt gtcagaattt tcttttcttc ctctcctaag gaaccagata caagcaggga
    10681 ttccagtatt ataaaaaagc tgttttggta aggcgacaat acattctacc agtccgtttt
    10741 ggatcagatt ttttctaatt tctccttctc ctcccgtatt ggaacttaga cttccgtttg
    10801 caagcaccgt tgccatcact cctgtaggct tgagatgata aagcatatgt tgcatccaag
    10861 caaagtttgc atttcatgca ggaggaagtc aatattgcca tcttgcatct ccttggagca
    10921 attcttgccc ccaatcagac tgattaaaag gcggatttgc taaaacgaaa tcagctttga
    10981 gatctggatg cgcatctttg agaaaacttc cttctgtatt ccaagctaca aactctgaat
    11041 tgatatggcg gattgcaaga ttcatttttg aaagtttcca agtagtctga ttggattctt
    11101 gcccataaat cgtaatatct tgcacatttc cttgatgttc tttgacaaat ttttcactca
    11161 tcacaaacat tccaccactt ccacaagcag gatcatacac tagtcactta taaggctcta
    11221 tcatttctac cattagcttt acaatcgctt ttggtgtgta gaattgtcct cattttttcc
    11281 cttcagcgtt ggcaaattct ccaaggaaat attcataaac tctcccaaaa agatctttgg
    11341 aagactggct ttgttctttg agttcagtat tggaaattag atcaatcaaa ccaccaagag
    11401 acatcacatc taaatttggc tttcaaaata cttttggcaa aacatttttg agctgaggat
    11461 tttctttttc tatcgcatcc atagcttgat ctagttcaat tcctattgtt ggtaattttg
    11521 ctcttgcttg gagatgattc catctcgcct gttgtggcac gaaaaaaaca ttttttgcgg
    11581 tatactcatc tctatcttct ggatcatcat aaggactaaa agccttattt gcgagttgat
    11641 cataaagttc ttgaaagcta tcggaaatat acttgagaaa aatcaatcca agcacgacat
    11701 ttttgtattc tgcagcatca atattttttc ttagtttgtc tgctgcttta aaaagctgtg
    11761 cttcaaaatt gccattttgt tctttagcca taagtttatc atgaaaattt aaaattttat
    11821 attgatttga attttattta ttttgtattg aaaagcaagt aaagaagtat taaaataatc
    11881 aattattaat ttattatttg gtctttatct tagtaaaaat tcttgtttat ttttgatgga
    11941 attttgaata aacaaaaaaa aacaggaccg gcaggaattg aacccgcgac gcggagcttt
    12001 gccccgcaat tctgcgggga taccactgag ctactgaatt ttgaataaac aaaaaaaaac
    12061 aggaccggca ggaattgaac ccgcgacgcg gagctttgcc ccgcaatcct gcggggtacc
    12121 actgagctac ggagtttttt aaagtaaaca aaaaacaggg atagagggat tcgaaccccc
    12181 gacacgcggc tttggagacc actgctctac cgattgagct atatccctat tctattttcg
    12241 ttttttaaat ttgtctaaac tataagcctg attatgtgtt atggtataat atatctaaac
    12301 tatcttgtag atagtattgt gcactacttc agcactaaat ttgtacaatt ctaaatttat
    12361 attatacaaa tttagtgctg aataagctta catcgattac ggtttacccc tcttcaaaca
    12421 atctgtttga ctgagtagaa gtagccctaa aaggacacct tcacgttgca tctttcccct
    12481 agcactaaag tttggtgctg ggtagttttc gtttctgtgg cactttcgtt tgctcccgat
    12541 ggtcgggacg gtccagcatc cattagatgc taatcttttt ttgatgtcag gactttcctg
    12601 cctagttttt actagactaa taccttgttt agacagatta cttttaatta aattcattca
    12661 aacttcaagt agaaataaat tttagtttat ttgtttttat aattttatta ctttgaattg
    12721 gatatatatc tctagtaatg tgtacatata aaataaaaat gtaatttatt tccaagattt
    12781 tggtttgatt atgtgttttc aatttccaat atctatctta tttggtaata agctattttc
    12841 aatatgttta gaatcgatat ttaaacgaga actacaggtt tttactatca tttttcattt
    12901 agatcgtgga aaaagtagag aaacaaattc tttatttcag atttttcact tttttgatag
    12961 atcacggact cttcttatag ctggtatgat atcactatct catgaaacta taatagcttt
    13021 gtcataatta tcaaaaactc aatcttcaaa tatttttagg gcaatattta catctgtttc
    13081 tttttcttca taagttataa attttagcaa gtctggaaca tcattatctt ttggttggat
    13141 ttcttctaaa acattttttc agtttttgaa agttttttct actctattaa attttcataa
    13201 aattacatca gttcacattt ttctgagagc ttgaatgtaa gttttatgtc tttttctggc
    13261 tggtttatcc cattctgaaa gtgctgtaaa aaaataaacc ttatttaatt tctcatcatc
    13321 atttaaataa ctttctgcta actttcttaa attacaccat ttatatttat tagaccaact
    13381 tgatcattct tcttcaattt ttttgattaa agaatgatag tgattaaatc catctatgta
    13441 aacattaaca atttttttat ttcaaaaaaa accacacgtg aggtcgtcac atgtgggtgg
    13501 acagacttcc gaatggaatg ctgtctcgtg tgtattatca ttataagaaa atttttttat
    13561 tttgcaagtt ttttattact tttttttgat gaccaaaata tattgttgtt taacttcatt
    13621 ttcaaattaa tctgctaaaa tatttctttt ataattatct tattgttatt gtcaaacctt
    13681 ttttagacat ttatttatac ccctgtttca attgtgttgt tagcaaatgt ttttaattaa
    13741 aacatggaaa attttgtatt aataatgagt tttatcgtct gataatttac aaaattgtct
    13801 atattctaac gataaataga tgttttaaga gaaaagagaa tgcaaatttt attatatgtt
    13861 ttgtctttgt tttcaacttc tattgtatat aaaaaccttt ttcataaaaa taa
//
LOCUS       DAOSID010000013        10951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_5899_length_10951_cov_13.473660, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000013 DAOSID010000000
VERSION     DAOSID010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 10951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10951
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5899_length_10951_cov_13.473660"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2535)
                     /locus_tag="PK674_01395"
     CDS             complement(<1..2535)
                     /locus_tag="PK674_01395"
                     /inference="COORDINATES: protein motif:HMM:NF016858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15218.1"
                     /translation="MQKSKFDGILVTLASVEDISKRSNGAVDNPDTVNYRTGRPKQYG
                     LFCETIFGPVKNYECSCGKYKGVRYKGIVCERCGVEVTTSRVRRSRMGHIDLASPVVH
                     AWYKSSPSGGIHHLLELSSNEIDRILTFVKYAVPEKITDEQRETLTIKLQETLDQKLI
                     DLEDLYKSELEEAGIDKKKLREIDKLYNENKEKLSLEFNRLKSIVSDLDFGKTVLESD
                     YRNVFCKYSDIVKFVSGPEGILKMLQNIDVKKEIKNRLDQYLKLKSVEQKKKLMSLIK
                     LLINLYVSGVKPENMVLRKLPVIPPDLRPVVQLDGGKFASSDVNLYYRRVLMRNLRLR
                     KMIQVGMPDVVKKNEIRLLQESVNNLLVGERGGPARGGAGVKVFKSLSDMLSGKEGIF
                     RKNLLGKRVDYSGRSIITVGPNLRLDECGLPISIAVTMFTPFIIGKLIEKKIVYTPKQ
                     AEKLIKEESPIALKFLEEVIKDKYVLLNRAPTLHRLSIEAFKIKLMPGKTIRIHPLVC
                     SAFNADFDGDQMAVHLPLSDEAQKEAREFIAADKNILRPGSGEPTITHSQDMVLGVYY
                     LTDFFNKKYPDYNTEEEREHKTPVVGYFESMDSVLKSFKNGDIHEKDKIVVKFNGESL
                     KTTVGRVIFNSVLPEKVQFENRTHKKKELKNLLSRIFDTYDMATTVQVADDIKDLGFH
                     YATVAANSINIADMRVPKEKENIIKQGEENANNIYKYFFKGFFSESEKHRLIVEIWTK
                     VKIDVEQHLKNIIGPGNDLYSMVDSGARGSQIHMTQISGMKGLVVNPKGEIIELPIKG
                     SFVEGLKPTEYFISAHSGRKGKADTALRTAESGYLTRKLCDASQEVV"
     gene            complement(2655..6290)
                     /locus_tag="PK674_01400"
     CDS             complement(2655..6290)
                     /locus_tag="PK674_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15219.1"
                     /translation="MSTKKTIKKTSISAKVQDSKIPAVLQNLFQKDGRYYLNTPKNFE
                     DYPDLLALQHKGYQDFVEKYVHNLFEDINPIRDIAGEKMYISISDIKISEPQQDIEEC
                     KRKELTYGGVISGKVKLVENMEDEESGKITEKVLFSKRANIGTLPVITPYASYIVNGV
                     ERVIISQIIRSYGIFYNQKDFGFGFKLIPERGSWIETKIEKSGVIVVRINKSRKFPIT
                     TLLRVFGLETDESIRESFEAIDEDDTDFIDITLKKDSTTDALSAAEFIYNKLRPGELI
                     DSYSALDYIKGQFLNPDRINIGRIARRKINAKLGLRKDLNIPESNVFDGVDLVAAIQY
                     LLNLSNHKKGYYIDDSDHLSNKRVRTMGEILYAHLGPVMRKFVKSVKGKLSVLNMENP
                     LKITDLVNFKIIDNSIKSFFATSQLSQFLDQINPLSELEHKRRITALGPGGLKRETAK
                     FEVRDVHPSHYGRICPIETPEGQNIGLVTYQSLFSHINEEGFLETPAFRVYKDVPAKK
                     DKLINRIADRDIFELDVKGNPTKTIIIKEDKLIDEKSAEKIEKFYGKLNTSINVKPFI
                     SDEVEYISPELDEKYFVADATTSTDKYNNITSKRVAARHFDNMVTLHVKDITHVDINP
                     SQIFSPNTSLIPFLDHNDAVRASMGTNQNRQGLPLLKNDSPLVSTGLDDMIKQTHVVI
                     MAEADGEVIYVDGKRVKVKYKTGLKEYKLTNYKRSNAKTCIHQVPRVSLGQKIKKGDI
                     IAEGPCSEGGELAIGKNLKIAFMPWKGYNYEDAIVISQRLVKNDTLTSVQIEEREIEV
                     SDTKLGPEETTNDIPGVSMSKLKNLDEDGIIRIGSIVKGGDILVGKITPKSEGELTPE
                     EKLIQAIFGDKSKNVKDTSLYVPSGSEGKVIDVVILDAKKGDNLMAGVRKKIKVYVAS
                     TRKIEVGDKLAGKHGNKGIIAVVVPEEDMPYTKDGEPIDLILNPLGVISRMNLGQLFE
                     VQLGYIAKKYGIKFAVGAFSGFGLEEFTKLIQELGIEDEINTQLYDGETGEPYNNKVT
                     VGYMHLLKLVHMVEDKIHARSVGPYSLITQQPLGGKSRQGGQRFGEMEVRALEAYSAV
                     YTLQEILTVKSDDVIGRNKMYESIIKGQKLNIGGLPESFNLVTYLFKGLGQNIQPMTQ
                     DELNEITQERFNKIRKLGLGSLMKEGVVVADEEGIENEEEVVPEEKGDIMDKILEDME
                     ESGELDG"
     gene            complement(6402..6557)
                     /locus_tag="PK674_01405"
     CDS             complement(6402..6557)
                     /locus_tag="PK674_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="bL34 family ribosomal protein"
                     /protein_id="HOG15220.1"
                     /translation="MASKLRRVRKYKWLKRLRTHGFREKMKKRTGRRILSNRRKKGRK
                     QLTPQRP"
     gene            complement(6633..7196)
                     /gene="rpsR"
                     /locus_tag="PK674_01410"
     CDS             complement(6633..7196)
                     /gene="rpsR"
                     /locus_tag="PK674_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="30S ribosomal protein S18"
                     /protein_id="HOG15221.1"
                     /translation="MQKYELVFMLNSQVKDSERKDFLSKFEEEFKKNILQKDDIGLKE
                     TSYDVKSIRGNNRMYYVSYYMDLNNDGLDSVKKSLLYSNIIVRYQIFKMDSTQIFFEF
                     EKLQKELQKIVDSRDNKKFGNRVSFLSHPENEKYINWKSIVILKKYLTRFGSIKPRKY
                     TKNRVKTQKKLRKAIIRARGLGLLSFV"
     gene            complement(7229..8029)
                     /gene="tsf"
                     /locus_tag="PK674_01415"
     CDS             complement(7229..8029)
                     /gene="tsf"
                     /locus_tag="PK674_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="translation elongation factor Ts"
                     /protein_id="HOG15222.1"
                     /translation="MSIDINLLKQLRETTFAPLKDCKDALIEANGDLQQAQEILKEKG
                     VLKAGKRSDRETKEGIVKVVSQNGKIAGVKLLCETDFVAKNEGFIKLCDDILDKLLSE
                     NEFFLSKQDAPTNLSESLDVLVKEAVGAIGENMQLDDIILTNENGFVYNHPGNKVASI
                     VFFEGANSDIAKEIALQVAAMNPSYLDFDSVPSDIISKMESQFRQELLDSGKPENILD
                     QILKGKLTKALAEDVLLEQEYIRDGSKKIKNIIPEGFQLKSYIRLAVK"
     gene            complement(8054..8566)
                     /locus_tag="PK674_01420"
     CDS             complement(8054..8566)
                     /locus_tag="PK674_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15223.1"
                     /translation="MKIKNPFQSTATNVKAMDNNYMPSSLEKKRAIMMYLFFGIMVSI
                     AKKDISIFEHYHLKQASGRWIVFFLVLVFDMVLLFLPIIKYLGVIPLLILLTVWIISV
                     KQAWDGKYFTNSKESFVANFSALGSWFIELFEIPSPSKPHNLPTTSDIESDIQNQTNK
                     SEIEKDLEKK"
     gene            complement(8580..9239)
                     /gene="rpsB"
                     /locus_tag="PK674_01425"
     CDS             complement(8580..9239)
                     /gene="rpsB"
                     /locus_tag="PK674_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="30S ribosomal protein S2"
                     /protein_id="HOG15224.1"
                     /translation="MSVTVKQIVDSQVHIGTLKSEAHPKTSNYRLDIVNNIVVLNPEM
                     ILEQLNKAKKKVQDVKKSGQEVLVVCEKKMYATEMKTLGERYGVSYLNYKAPGGFLTN
                     FDTFKTRISSMNKMSAFVETEEFASLTKKEQLVYKRKLSRTKKIYEGVKNLTTKPGLV
                     IVVDGSMMRNFVRELPAQGAVDSIVISSTNYDQYLGDNDIIANMLSYKSIDFVMNYIL
                     S"
     gene            complement(9343..9729)
                     /gene="rplL"
                     /locus_tag="PK674_01430"
     CDS             complement(9343..9729)
                     /gene="rplL"
                     /locus_tag="PK674_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOG15225.1"
                     /translation="MELSKNAQKIMELVKEMSAIELNELVKAFEEEFGVTATATVVAT
                     GSPAGGDATVATGGDVAANIELTEIGQQKINVIKVVKELLGLGLKEAKDLVEKAPVIL
                     KENAKDDEVESFKIKLQEAGATVTIK"
     gene            complement(9863..10519)
                     /gene="rplJ"
                     /locus_tag="PK674_01435"
     CDS             complement(9863..10519)
                     /gene="rplJ"
                     /locus_tag="PK674_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L10"
                     /protein_id="HOG15226.1"
                     /translation="MAITKEKKINLIKEYVKDLQEAKNLVIIRQSGLAVSTDMEVRKN
                     VRNESGKFNVIRKRLFLRALKEAGYPEIDLSQIPGSAVAVYAKGEEYAPLKVINKYIK
                     TFQKDKDSTSSFEFLLGYFDGERKDLNYIDELANIPSREELLSKLVRLFNYPVQSLTS
                     VLNQIAKKKSEEIQEEKSSEETQEEKSFEETQEEAKEEAVSTTDTELAENSKAQTTSE
                     "
     misc_feature    complement(10616..10749)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
BASE COUNT         3362 a         1870 c         1377 g         4342 t
ORIGIN      
        1 aacaacttct tgagaagcat cacaaagttt tctagtaaga tatcaagatt ctgctgttct
       61 aagtgcagta tctgctttcc cctttcttca tgagtgagca gaaataaaat attctgtagg
      121 ttttaatcat tcaacaaagc ttcacttgat agggagttct ataatttctc attttggatt
      181 aaccacaagt catttcatac cagaaatttg tgtcatgtga atttgagatc atcttgctcc
      241 agaatcaacc atactataaa gatcatttcc aggtcaaata atatttttta aatgctgttc
      301 aacatcaatt ttaaccttag tccaaatttc cacaatcaat ctatgcttct ctgattcaga
      361 aaaaaatcac ttgaaaaaat atttatatat attatttgca ttttcttctc attgtttgat
      421 gatattttcc ttttcttttg gaactctcat atctgcaata tttatagaat ttgcagcaac
      481 tgtagcataa tgaaatcaca aatctttgat atcatctgcc acttgtacag tagtagccat
      541 atcataggta tcaaatatcc tactaagtaa gtttttaagt tcttttttct tgtgggtacg
      601 attttcaaac tgtacttttt ctggtaacac agaattaaaa ataactctac caacggtagt
      661 ttttagagat tctccattaa attttaccac tattttatct ttctcatgta tgtctccatt
      721 tttaaatgat ttcaaaacgc tatccataga ttcaaagtat caaactactg gtgttttatg
      781 ttctcgttct tcttcggtat tgtagtctgg atattttttg ttgaaaaaat ctgtcaaata
      841 atatactcac aataccatat cttgagaatg ggtaatggta ggttctcaag atcatggcct
      901 taatatattt ttatctgctg caataaattc tctagcctct ttttgagctt catctgataa
      961 tggaagatgt actgccattt gatctcagtc aaaatcagca ttaaatgctg aacaaactaa
     1021 tggatgaatt ctaatggttt ttcatggcat tagttttatt ttgaaagctt ctattgaaag
     1081 tctatgaagt gtaggagcac ggtttaaaag tacatactta tcttttatta cttcttccaa
     1141 aaatttaagt gctattgggg actcctcttt tattaatttt tccgcctgtt ttggtgtgta
     1201 aacgattttt ttctctatta attttcatat aataaaggga gtaaacattg taactgcaat
     1261 tgatataggt aaaccgcatt catccaatct caaatttggt caaactgtaa taatagacct
     1321 tccagaataa tccactctct ttcaaagtag gttttttcta aatattcatt cttttccaga
     1381 aagcatgtcc gaaagagatt taaaaacttt aactccagct cctcacctag ctggtcatca
     1441 tctttctcaa accaataaat tatttacaga ttcctgtaaa agccttattt catttttctt
     1501 tacaacatca ggcataccaa cttgtatcat ttttctaagt cttaaatttc tcatcaaaac
     1561 tcttctataa tacaagttca catctgaaga tgcgaatttt catcaatcta attgtacaac
     1621 cggtcttaaa tcaggtggga taactggtag ttttcttaaa accatatttt ctggtttaac
     1681 tccagaaaca tataagttga taaggagttt aattaaagac atcaactttt ttttctgctc
     1741 tacagatttt aatttcaaat attgatccaa tctatttttt atttcttttt ttacatcaat
     1801 attttgtaac atttttaaaa ttcattctgg tccagaaaca aattttacta tatcagaata
     1861 tttgcagaaa acatttctat aatcgctttc caatacggtt tttcaaaaat ccaaatcaga
     1921 aacaatggac tttaacctat tgaattccaa agacaatttt tctttatttt cattatataa
     1981 tttatcaatt tctcttagct tctttttgtc tataccagct tcttccaatt ccgatttgta
     2041 taaatcttct aaatctatca atttttgatc taaagtttct tgtaatttta tagtcaaagt
     2101 ttccctttgt tcatccgtaa tcttttcagg aacagcatat tttacaaatg tcaaaattct
     2161 atctatctcg tttgaagaaa gctctagtaa atgatgaatt catcaagatg gactagattt
     2221 gtaccaagca tgaactactg gagatgctaa atctatatgt cacattctgg atcttctaac
     2281 cctagaagta gtgacttcta ctcaacatct ttcgcatact attcatttat atctcactca
     2341 tttatatttc ccacaactac attcatagtt ttttactggt ccaaaaatag tttcacaaaa
     2401 taatcaatat tgttttggtc ttcaagttct ataatttact gtatctgggt tgtccaccgc
     2461 cccattggat cgtttcgaga tatcttccac agaagcaagt gtcacaagaa ttcaatcaaa
     2521 tttagatttt tgcattagaa atagtttaaa caaaaataaa tactatagtt catcaagttt
     2581 gtaaagtttg taaagttcat aaagtattaa ttaacttgca actttataaa cttttaactt
     2641 tcaacttgtt actcttaacc gtccaattct caactctcct ccatatcttc caatatttta
     2701 tccataatat ctcacttttc ttctggaacc acttcttctt cattttcgat tccttcttca
     2761 tcagctacta ctactcattc tttcattaaa cttcataatc caagttttct aattttatta
     2821 aatctttctt gtgtaatttc atttaattca tcttgagtca taggttgaat gttttgtcat
     2881 aatcacttga acaagtatgt aactaaatta aaagattctg gtaatcctca tatgttcaat
     2941 ttttgcccct ttataattga ttcatacatt ttatttcttc atattacatc atcagatttt
     3001 actgttaata tttcttgtaa agtgtataca gctgaatacg cctccaatgc tcgcacctcc
     3061 atttctccga atctttgtca tcattgtctt gattttcatc ataggggttg ttgagtaatt
     3121 aatgaatatg gtcaaacaga cctagcatga attttatcct caaccatatg aactaacttc
     3181 aataaatgca tatatcaaac tgtgacttta ttgttatatg gctctcaagt ttctcaatcg
     3241 tataactgag tatttatttc atcttctatc ccaagttctt ggataagctt ggtaaattcc
     3301 tctaatccaa accccgaaaa tgctcaaaca gcaaatttaa ttcaatactt tttggcaata
     3361 tatcaaagtt gaacttcaaa cagctgtcac aaattcatac gagagataac tcacaaggga
     3421 tttaaaataa gatctatcgg ttctcaatct tttgtatatg gcatatcttc ttctggcacg
     3481 acaactgcaa taataccctt gtttccatgt ttacctgcca atttatctca aacttcaatt
     3541 tttcttgtac tagccacata tacttttatt ttttttctaa ctcaagccat caaattatct
     3601 cacttctttg catccaaaat aaccacatct atcacctttc actcactgcc agaaggcaca
     3661 tacaatgatg tatctttcac atttttagac ttatctccaa aaatagcttg tatcaacttt
     3721 tcttctggag tcagctctca ttctgatttt ggggttatct ttcatactaa aatatctcat
     3781 cactttacta ttgatcatat tctaattata ccatcttcat ccaaattctt taactttgac
     3841 atagaaactc atgggatatc gtttgttgtt tcctcaggtc ataatttagt gtctgaaact
     3901 tctatttctc tttcttcaat ttgtactgat gtcagagtat cattctttac taatctttgt
     3961 gaaataacta tagcatcttc ataattataa cccttccatg gcataaatgc tatttttaaa
     4021 tttttaccta tggctaattc tcatcattca ctacaaggtc attctgctat aatatctcat
     4081 tttttaatct tttgtcatag agaaactctt gggacttgat gtatgcaagt ttttgcgttt
     4141 gatcttttat agtttgttaa tttatattct ttcaatcagg tcttatattt aactttgact
     4201 ctttttcaat ctacatatat cacctcccca tcagcctctg ccattatcac cacatgagtt
     4261 tgttttatca tatcatctag tcaagttgaa accaaaggac tatcgttttt tagtagtggt
     4321 aatcactgcc tattttggtt tgttcccatt gaagcacgca cagcgtcatt atgatccaaa
     4381 aatgggatta aagatgtgtt tggtgaaaat atttgcgaag gatttatgtc tacatgagtg
     4441 atatctttta catgtaaagt caccatatta tcaaaatgtc ttgctgcaac gcgcttactt
     4501 gtgatattat tgtatttatc tgttgatgtt gtagcatcag ctacgaagta tttttcatct
     4561 agttctgggg aaatatattc cacttcgtcg ctaataaatg gcttaacatt gatactagta
     4621 tttagttttc aataaaactt ttctattttt tctgcagatt tctcatctat taatttatcc
     4681 tcttttatta ttattgtttt tgttggattt cattttacat ctaattcaaa aatatcacga
     4741 tctgcaattc tgtttattaa tttatctttt ttggctggga catccttgta aactctgaat
     4801 gctggtgttt ccaaaaatca ttcttcattt atgtgagaaa ataaggattg atatgttact
     4861 aatcatatat tttgtcactc tggagtctca attgggcaaa ttcttccata atgagatgga
     4921 tgtacatcac gaacttcaaa cttagctgtt tctcttttta atcatccagg tcatagtgct
     4981 gtaattcttc ttttatgttc taattccgat aaaggattga tttggtccaa aaattgggat
     5041 aattgtgaag ttgcaaaaaa actctttata gaattatcta ttattttaaa gtttacgaga
     5101 tcggtaattt ttagaggatt ttccatgttt aatactgaaa gttttccttt tactgatttt
     5161 acaaattttc tcatcacagg tcataaatga gcatacaata tctctcacat tgttctgacc
     5221 cttttgtttg aaaggtgatc tgaatcatct atgtaatatc attttttatg gttagataga
     5281 tttaataggt attgtatagc tgctactaag tctactcaat caaatacatt tgattctggt
     5341 atgttaaggt cttttcttag tcataatttg gcattaattt ttcttcttgc tattcttcca
     5401 atatttattc tatctggatt taaaaattgt catttaatgt aatccaatgc actataacta
     5461 tctataagct ccccaggtct gagtttgttg tagataaatt ctgctgcaga taatgcatct
     5521 gtcgtagaat ctttttttaa tgtgatatca ataaaatctg tgtcgtcctc atctattgct
     5581 tcaaaagatt ccctaatact ttcatctgtt tccaatcaaa atactctaag tagagtggta
     5641 atcgggaatt ttctagattt atttatccta actacaatca ctcccgattt ttctatttta
     5701 gtttctatcc aagaacccct ctctggaatt aatttaaatc aaaatcaaaa gtctttttga
     5761 ttataaaaaa taccgtaaga tctaataatt tgtgaaatta ttactctttc tactcagttt
     5821 actatgtaag aagcatatgg ggttataacc ggtaatgttc aaatatttgc gcgttttgaa
     5881 aaaagcactt tttctgtaat tttacccgac tcctcatctt ccatattttc cactaattta
     5941 acttttcaag aaataactca tccataagtt aattcctttc tcttgcactc ttcgatatct
     6001 tgctgaggtt cagatatttt gatatctgaa atagatatat acattttttc tcatgcaata
     6061 tctcgtattg ggtttatatc ttcaaaaaga ttatgaacat acttttccac aaaatcttga
     6121 tatcatttgt gctgtaaagc cagtagatct ggataatctt cgaaattttt tggtgtattt
     6181 agataatatc taccatcctt ttggaataga ttttgtaaaa ctgcaggtat tttgctgtct
     6241 tgaacttttg cagaaataga ggtttttttt atagtttttt ttgttgacat attgtttgaa
     6301 atttttaggg gaagtaaata tttgaacaga tcgcttaata attgatggat atgattaaga
     6361 tattttgatt tcttcaaaaa aatcttagta tgaaaaatac attaaggtct ttgtggtgtt
     6421 agttgttttc tacctttttt tcttctatta gatagaattc ttcttcctgt ccgtttcttc
     6481 atcttctctc taaatcaatg tgtcctaagt ctttttagcc atttatattt tctaactctt
     6541 ctcaacttac ttgccatttt agcttatttc aaaataaaaa tattttatgt ttttgaatcc
     6601 tatttttttt gaagcttgtt tacaatgtgt ttttaaacaa aactcaataa tcacaaacct
     6661 ctggccctaa tgattgcttt tcttagtttt ttttgggttt ttacacgatt ttttgtatat
     6721 tttctaggtt ttatagaacc aaatctagtt aaatactttt ttagaataac tatggatttc
     6781 cagttgatat atttctcatt ttcaggatga gataagaatg atactctatt tccaaatttt
     6841 ttattatctc gagaatcaac aattttttgt aattcttttt gtaatttttc aaattcaaaa
     6901 aatatttgag tagaatccat tttaaaaatt tgatatctta cgataatatt ggaatatagt
     6961 aaagactttt taactgaatc caatcaatca ttatttaggt ccatataata ggaaacataa
     7021 tacattctat tgtttcacct aatagatttc acatcatagg atgtctcttt taggccaata
     7081 tcgtctttct gaagaatgtt ttttttgaat tcttcttcga atttagataa aaaatctttt
     7141 ctctctgaat ctttgacttg agaatttaac ataaaaacta attcgtactt ttgcatttta
     7201 atttaatcac aaatataaat ttgatatatt attttacagc caatctgata taagacttaa
     7261 gctggaatca ttctggaatt atgtttttta ttttttttga accatctcta atgtattctt
     7321 gttccaataa aacatcttca gccaaagctt tagttagttt tcattttaat atctgatcaa
     7381 gtatattttc tggtttccca gaatctaaca gttcttgacg gaattgagat tccattttag
     7441 aaataatatc gcttggaaca gaatcaaaat ctaaatatga gggattcata gcggcaactt
     7501 gtaaagctat ttcttttgct atatcagaat ttgctcattc aaaaaacact atagatgcaa
     7561 ctttattacc tggatgatta taaacaaaac cattctcatt tgtaagtata atatcatcta
     7621 attgcatatt ttctcatatt gctcacacag cctcttttac caacacatcc aaactctcag
     7681 ataagtttgt gggagcgtct tgttttgata agaaaaactc attttctgat aatagtttgt
     7741 ctaagatatc atcacataat ttgataaatc actcattttt ggcaacaaaa tcagtttcac
     7801 ataaaagttt aactcaagct attttgccgt tttggctcac aacttttaca attcattctt
     7861 ttgtttctct gtctgatctt ttaccagcct ttaaaactca tttttctttt aaaatttctt
     7921 gtgcttgttg caaatcccca tttgcttcta tcaaggcatc tttacaatct tttagaggag
     7981 caaaagtagt ttccctaagt tgttttaata agtttatatc tatactcatg gtatttgttt
     8041 ttataattaa aaattatttt ttctctaaat ccttttctat ttcggattta tttgtttgat
     8101 tttggatatc agactcaata tcgctagttg ttggtaagtt gtgtggcttt gaaggtgagg
     8161 gtatttcaaa caattctata aaccatgatc ataatgcaga aaagtttgca acaaatgatt
     8221 cttttgaatt tgtaaagtat ttcccatccc aagcctgttt tacactaatt atccaaacgg
     8281 ttaataatat caataaagga ataactcata aatactttat aataggcaaa aacaataaaa
     8341 ccatatcaaa tactaacacc aagaagaata ctatccaccg accagaagct tgcttcagat
     8401 gatagtgctc aaaaatactg atgtcttttt ttgctataga aaccataata ccaaaaaaca
     8461 agtacatcat gattgctctt tttttttcta agctactagg catatagttg ttgtccatag
     8521 ctttcacatt tgtagcagta gactgaaaag gatttttgat tttcatatat ttttttagat
     8581 taggataaga tataattcat aacaaaatct atacttttat aacttagcat atttgcgata
     8641 atgtcgttat ctcacaaata ttgatcatag tttgtggaag atatcactat actatcaaca
     8701 gctcattgag ctggtaattc tctaacaaaa tttctcatca tagagccatc tacaactatc
     8761 accaatcatg gtttagtggt aagatttttt actcattcat agatcttttt ggttctggat
     8821 aattttcttt tgtaaaccag ttgctccttt ttggtcaaag aagcaaattc ttcggtttcc
     8881 acaaaagcac tcatcttgtt cattgagctg atcctcgttt tgaaagtatc aaaattggtc
     8941 aaaaatcatc caggagcttt gtaatttaaa taagatactc agtacctttc tcctaaagtt
     9001 ttcatttctg tggcatacat ctttttttca catactacca aaacctcttg tcctgatttt
     9061 ttgacatctt gaaccttttt ttttgctttg tttagctgtt ccaaaatcat ttcaggattt
     9121 aaaacaacta tgttgtttac aatatccaat cgataattgc ttgttttggg atgagcttca
     9181 ctttttaagg taccaatatg cacttgtgaa tctactattt gttttacagt aacactcatt
     9241 attggttttc agaattaaat aaattttttc taaagctttt ggtactaaat acttttttag
     9301 ttttttaaaa acaaaaatca agtaagtgtg ccaaaattta gactacttta tagttactgt
     9361 tgcaccagct tcttgtaact tgattttgaa cgattctact tcatcatctt ttgcattttc
     9421 ttttagtata acaggagctt tttcaactaa atctttggcc tcttttaatc aaagtcacaa
     9481 taattctttt acaactttaa ttacattgat cttttgttga ccaatttctg taagttcgat
     9541 gtttgcagcg acatctcatc cagtggcaac ggtggcatct catccagctg gacttcatgt
     9601 agcaactaca gtagcagtag cagttacgcc aaactcctct tcaaaagctt taacaagctc
     9661 atttagttcg attgcactca tctcttttac caattccatg attttttgtg cgttctttga
     9721 taattccatt ctcaatacta atttaataaa taaatatttg taagtttaca atattagttg
     9781 ttccaatagc ttataaggac aaaatatcac aaacattttg acctaataag caattgaaaa
     9841 caaaaaacat tgaataattt ctctactctg aagttgtttg tgctttagaa ttttctgcaa
     9901 gttcagtatc tgttgtagaa acagcctctt cttttgcttc ttcttgagtt tcttcaaaac
     9961 ttttttcctc ttgagtttct tcagaacttt tttcctcttg aatttcttca gacttctttt
    10021 ttgcaatttg atttaaaaca ctggtaagcg attgaacagg ataattgaat aatcgaacca
    10081 atttagatag caattcttct ctacttggta tatttgccaa ttcatcgata taattcaaat
    10141 cttttcgctc accatcaaaa tatcataaca aaaactcaaa acttgatgta gaatctttat
    10201 ctttttggaa agttttaatg tatttattta ttactttcaa tggagcatat tcctctcatt
    10261 ttgcataaac tgcaacagca gatccaggaa tttggctaag atctatttct gggtatcctg
    10321 cttcttttaa ggccctcaaa aaaagtctct ttctgataac attaaattta ccactttcat
    10381 tcctaacatt ttttctaact tccatatctg tggaaacagc taatccagat tgtctgataa
    10441 ttactagatt ttttgcttct tgtaaatcct tgacatactc cttgattaaa tttattttct
    10501 tttctttggt aatagccatt ttagaattca aagtttaagt tttaaataag atttggacaa
    10561 gtcctaatag attaagattt ttattttaat acattttgaa caaaaatctt gttggttttt
    10621 gaattaaaaa aaaccacatc cgaagatgaa gttatataca aaaaaataat ttttgatata
    10681 acctaggtag gagcttactt ttctaaaatt agaatagcct actgtctccg gcgatcagcg
    10741 aaaatatata aatttataat caaaatgcaa tagaaaatga aagatttttg ataaaattag
    10801 tttaatataa atatatttag aaatggacaa agaaatttag attaatattt catgcttaaa
    10861 taaaaaaaaa tgattagcga ctttttttta ttatttctca gtactgtccg ggaaaatttt
    10921 agatataaga aaataatgaa aattgaggag g
//
LOCUS       DAOSID010000014        10630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_6110_length_10630_cov_14.118392, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000014 DAOSID010000000
VERSION     DAOSID010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 10630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10630
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6110_length_10630_cov_14.118392"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..550)
                     /gene="cas2"
                     /locus_tag="PK674_01440"
     CDS             complement(272..550)
                     /gene="cas2"
                     /locus_tag="PK674_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOG15227.1"
                     /translation="MLIVSYDISNNKLRTRFSKFLEKYGRRLQYSVFEIKNSDRILNI
                     ILLKIEKTFSKKFEMTDSILILNLCGPCEKKVYRYGYAVHEEEELIFA"
     gene            complement(560..1513)
                     /gene="cas1"
                     /locus_tag="PK674_01445"
     CDS             complement(560..1513)
                     /gene="cas1"
                     /locus_tag="PK674_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="type V CRISPR-associated endonuclease Cas1"
                     /protein_id="HOG15228.1"
                     /translation="MLSAPDFKEKQILVISSDERKGLSLRNNNVLIKEDEKIVNQISC
                     SKIFCIFIIGDFTISSKLIDKLMKYQIPIYCLGYNLKPKFMIGNTLQGNYILREKQYN
                     NPNELDIAKMIIKNKATNQLSLLQEIRGKTDSMKDSIKKIKDTIKKIDSADNDDSLRG
                     IEGSIAKLFFSNYFEEMKWYRRMPRTRNDIINFLLDIGYSFLYNFIEANLNLYGFDVY
                     KGVYHKLFYERKSLVCDLVEPFRCIIDKKIRKMYNLGQINEKDFKFSKGEYSISREKQ
                     KSYIGNFLQALLDEKMEIFAYIKGYYRYMMDPKREFPIFTL"
     gene            complement(1557..2102)
                     /gene="cas4"
                     /locus_tag="PK674_01450"
     CDS             complement(1557..2102)
                     /gene="cas4"
                     /locus_tag="PK674_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="type V CRISPR-associated protein Cas4"
                     /protein_id="HOG15229.1"
                     /translation="MEQNIRISSLNDFLFCPKSIYFHNLYEDYERQVYQDTPQVKGTI
                     SHENIDNKHYSTSKNILQGFGIYSEKYNLVGKVDIYQTDKKTIIERKTKISKLYQGQI
                     YQVYAQYFCLTEMGYEVKKLKIHSLSDNKIYDIPIPNKEEIEEFEKFLDEFRKFDPSK
                     PFEANPEKCKKCIYRELCDSY"
     gene            complement(2124..5513)
                     /locus_tag="PK674_01455"
     CDS             complement(2124..5513)
                     /locus_tag="PK674_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15230.1"
                     /translation="MMEKLTNLYEVIKTIKFELKPSEFTLKRCSFDSSFESDPHVLFE
                     KLKPIHKQQKEILNILSNLPPQKIVIHKNLIKLIDSDYFYRLKSKALSANNTLVVLSK
                     EFQEEFSNYIKEKFDRLHSTNYSLSYLLWADSLEKPSNTDKKPTKSEIAFAVKRYVHA
                     VQKVEEIISNIIYKTGNTDNELKTMLKSYYDNKDLGYIFSIANRYLQSYSPTSGFQVS
                     KFGFNAKAIRKKVDVEKMEKEIRILETKMLDLEDKKVIATQIFDDYTKKFVQEKIKTD
                     PKLVALRENQTASPNERKQAKEYLKTLKEGDFNYQKILKERSKAKKEQKKYEPKFTGD
                     KEYCELKLAKIDASVIYGRAKGDFFAKQQELYSQQALSHYARLVRNGEKYFVVMIDRD
                     NINELDNLGDNQIKNDDYFEILRYSSLTAKAVEKLIFEKNALNFNRYDKEYRDCKNIR
                     EKRTYKEKGSEDSRSITNDQKTKLRKLVSFLNEAITQLNKKVEGGVFNWVPFNISNEF
                     DDFAEFRKYVTKTCYITKWESISKQIIYDLDKKGILDFYQIYNKDFAVDPYFAKTERD
                     KNRIKKDISIMGKQNLFTIYWESLFGDNSSNIRLNPESNLYVRPAKVVEEISDHNPNY
                     ETSKLRYKENKIIGDFQLVFYPTKELPLDKLQKKILDEVDKSYAIGIDLGENSLATIC
                     LIDNEKRVVLDEEGKPIIKDLSMINNKGDFVELDDCYIDGQPYKRKQKLLVGGQGNKP
                     YTLGRYFPEKFQNQVISKMSKETLIEVLKILEDIAPEKLGIVDNHGNKVFDYNYAFEH
                     LKATNKIRYHLQAFDHTNEEMEILEKDLLASTKIRGGFVGKVVSYITQMSKKYNALIV
                     FENLDQEIGRIRGMTLFSEQEYEKVSAKEERMYKGISPYQLIQDKIIQKCNYLLSKQD
                     NTKGIQISPRLKRQEDIKQLTLLKDKNCAWGKVVFVNEENSSKECPECGFIPGKYSSN
                     INIQENGIEIMANKKDKKLLLFENNPLLNERRIAYQSIPDTNELKKKSLLIETRVGKT
                     ILTETDMKIVGDPMICVNCGYDTRKEEWVKKYNLKSCDEIAAYIIAKRGLEFVENKGY
                     ENQSIDKPSQTNTFEENKKSKNSDNENSSYTMSGNINFSHS"
     gene            complement(5531..9298)
                     /gene="cas12a"
                     /locus_tag="PK674_01460"
     CDS             complement(5531..9298)
                     /gene="cas12a"
                     /locus_tag="PK674_01460"
                     /inference="COORDINATES: protein motif:HMM:NF037260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="type V CRISPR-associated protein Cas12a/Cpf1"
                     /protein_id="HOG15231.1"
                     /translation="MSDKKDIFSDFTNLYPLSKTLRFELKPVGKTNELLEGENPFPKD
                     KKINEIYQNIIKPCLNDLHSQLIEESLEGASLELDESVYDLRKGSIERHKSESEKKKS
                     KDDFDKAKKELRKQIVNYLKHSRIAKKYPKISGYEILGQKEIIPILAEIYGDRIYTKD
                     TTDKSKKYKNPDLIGKPYKELINDYFVGFFTYLSNFKDNRDNLYKDDGKASRVATRVI
                     DENMIRFFQNKKSYEKFVGFIDLNSDEQNCFDALCFQNHLNQKGIEIYNNIIGGINTK
                     INAYNQTNKGGEKLPKLIKLHKQILAKPDKESIFSFVNAIIEDDEDLEKNLKTFLEDA
                     KQRIEQSINSVLNALLEEKFDYEKTYFKSSALKDISNKYLNNWDVLGSLLPEDENADK
                     KSKGKKSDDVISLQEIKDALNSTETEGFLKKHWFDCLKEYNTSIAGDNAFIQLLKTIV
                     LDANIQKENLENYTNILEKNLFSGNFSTNLRNSEKDIKIGDLKMTAKTLIKETIQIVL
                     QFEQILRYFSLEKGQGKDKELIDVEFDSDFYNEIDKFYSDDFKPYKFFNAVRNYITKK
                     QYSTDKIKLNFDNSTFLDGRDKNKESDNFGVILRRKGEYFLAIMKKGENKIFKDSNHE
                     LYSDQGEKYEKLEYKLLPGPNKMLPKVFFSNKNIDFFNPSDKVLNIRKKESFKQGKDF
                     SLQDLHTRIDFMKSSLSIHPDRSKFGFYFKDTSEYKQINEFYSDVENQGYKVSFVPVS
                     GNTLHKYVEEGNLYLFQIYNKDFSEHRKGKENLHTKYWKALFEKENFNNGVFFKLNGR
                     AEIFFRPATVTEKKEKDLKKKNTGAIENKRFTENKILFHCPITLNFTNKNEFKINDKV
                     KEYAKKHTMNVIGIDRGEKHLLYYALIDQNGNILKQGDEKQLCSMNILTSKLPDGSKK
                     EVDYYDKLVKKEGDRAKERVDWNEIENIKEMKQGYLSHVVNKIVDLAIQNNAIIVLED
                     LNSGFKNSRKKVERQIYQNFELALAKKLNFVVDKNKADNELGGLYNAYQLTPKVDNFQ
                     DIYAQTGILFYTQAGYTSVTCPVCGFRKNVYKKYDSLASIQKWIKDIDLKIEKKGLDF
                     YFFYKLESLEDKKGNKREKLDRKITTKNQIRLFNERIEKGTAFKSTEIVLSEKFSDLF
                     KEYGIDENNIVKSMLEKSDVKLFKDFMFYRNLLLQIRNSKEGDDGGYLQCPACEFHSD
                     NGFQGTHFNGDANGAYNIARKGVMILNKINDNEKTLGITNVEWDNFTQKEF"
     gene            complement(9427..9621)
                     /locus_tag="PK674_01465"
     CDS             complement(9427..9621)
                     /locus_tag="PK674_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15232.1"
                     /translation="MSEIIEQLKNFEDRKVVLDYNGTITEGHIVWICDEEVVLRTDKG
                     RMITLEPSQIDLDSIKVLPN"
     gene            complement(9633..9842)
                     /locus_tag="PK674_01470"
     CDS             complement(9633..9842)
                     /locus_tag="PK674_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15233.1"
                     /translation="MEKLIKFEGKVVSLSYDGHLPDGIIPPVMLEGQLINVDRFECTF
                     KRKDTGEILYLLTPDIMIESIKIIE"
     gene            complement(10266..10487)
                     /locus_tag="PK674_01475"
     CDS             complement(10266..10487)
                     /locus_tag="PK674_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15234.1"
                     /translation="MKFLKTFERKKLILVYHGDINDDYIPEGIVAPFTMKGTLTVIGD
                     HKCVFKLENGKKFLFDTEKIDLESITVIE"
BASE COUNT         3313 a         1589 c         1219 g         4509 t
ORIGIN      
        1 atctacaaca gtagaaattt tttatgtttt ctaaatccca cttttggctt atttatgcta
       61 ttttctcggc gatcgtagaa gatcatcact ccgagaaaag tagcataaga ccatcttttg
      121 attttaggaa gatggtaggt ttacatattt atagaataca taacataatt tctagtaacc
      181 ttacggctac aattgtagtt ttttataaaa aatgttaatg atcacttaat tgcattcaat
      241 aatactgaat atttcagtaa attcaataga tttaggcgaa aattaattct tcctcttcat
      301 gaacggcata tccataccta taaactttct tttcacaagg tcagcatagg tttaatatta
      361 aaatgctgtc tgtcatttca aatttctttg aaaatgtttt ttcgattttc aaaagtatta
      421 tattcaaaat tctatcacta ttttttattt caaatacaga gtattgtagt cttcttcaat
      481 atttttctaa aaatttagaa aatctggtcc taagtttgtt gttactaatg tcataagaaa
      541 ctattaacat ctgtattttt tataatgtaa aaataggaaa ttctcttttt ggatccatca
      601 tatatctata atatcatttg atataggcaa atatttccat tttttcatct aataatgctt
      661 gtagaaaatt tcatatatat gatttttgtt tttctcgtga aatactatat tctcattttg
      721 aaaacttgaa atctttttcg tttatttgtc agagattgta cattttccta atttttttat
      781 caataataca cctaaacggc tccactaaat cacatactaa agactttctt tcataaaaaa
      841 gtttgtgata aactcattta taaacatcaa atcaataaag gtttaagttg gcttcaataa
      901 aattatacaa aaacgaataa ccgatatcca ataaaaaatt aattatatca ttcctagttc
      961 ttggcattct cctataccat ttcatttctt caaaataatt ggaaaaaaat aattttgcaa
     1021 tactaccttc aattcatctg agactatcat cattatctgc tgaatctatt tttttgattg
     1081 tatcttttat ttttttaata ctgtctttca tactatctgt ttttcatcta atttcctgta
     1141 ataaagacaa ttgatttgtt gctttatttt ttataatcat ttttgctata tccaattcat
     1201 ttggattatt gtattgtttt tctctgagaa tataatttca ttgaagagta ttaccaatca
     1261 taaatttggg ctttaagttg tatcatagac aataaattgg gatttggtat ttcataagtt
     1321 tgtctattag tttgctacta attgtaaaat ctcatataat aaaaatacaa aaaatttttg
     1381 aacatgaaat ttgattcaca attttttcat cctcttttat taaaacattg ttgttgcgta
     1441 atgataaacc tttccgttca tcagaactta ttactaaaat ctgtttttcc ttgaagtcag
     1501 gggctgaaag cattttctag aaactaaaaa agtaaaatat cgttttttaa taaaaactaa
     1561 tagctatcac aaagctctct atatatacat tttttacatt tttctggatt tgcttcaaat
     1621 ggtttacttg gatcaaactt gcgaaattca tctaaaaact tttcaaattc ttcaatttct
     1681 tctttgttgg ggatgggaat gtcataaatt ttattgtcgc tgagagagtg gattttgagt
     1741 tttttgactt catatcacat ttctgttaaa caaaaatatt gtgcgtaaac ttggtaaatt
     1801 tgtcactgat ataatttaga tattttagtt ttcctttcta taatcgtttt tttatctgtt
     1861 tgatagatat ctactttacc aacaagatta tatttttcag aataaatccc aaatcactgt
     1921 aatatgtttt ttgaagtaga gtagtgcttg ttatctatat tttcatgact tattgttcat
     1981 ttaacttgcg gtgtatcttg gtacacctgc ctttcataat cctcgtaaag attgtgaaaa
     2041 taaatggatt ttggacagaa aagaaagtcg ttgagggagg aaatacggat attttgttcc
     2101 attactctta tataaaaaat aaattaagag tgtgaaaaat taatattacc agacattgta
     2161 tatgaggaat tttcattatc agagtttttt gattttttat tctcttcaaa tgtgttagtt
     2221 tgactgggct tgtcgattga ttggttttca tatcacttgt tttcaacaaa ttctaatcac
     2281 ctttttgcaa taatatatgc tgcaatttca tcacaacttt ttaagttata ttttttgacc
     2341 cactcttctt ttctagtgtc atatccacaa tttacacaaa tcattggatc tcaaacaatt
     2401 ttcatatctg tttcagttaa aattgtcttt ccaaccctag tttctatcaa cagagatttc
     2461 ttttttaatt cgtttgtatc tggtatactc tgatacgcaa ttcgtctttc atttagcaga
     2521 ggattgtttt caaataataa aagtttttta tcttttttgt ttgccataat ttcaattcag
     2581 ttctcttgaa tatttatgtt acttgaatat tttcatggga taaatcaaca ttcaggacat
     2641 tctttagaag aattttcttc attaacaaaa acaacctttc accatgcgca atttttatct
     2701 tttaataaag ttagttgctt aatatcttct tgtcttttta atctaggtga aatttgaatt
     2761 cactttgtgt tatcttgttt agataaaaga taattacact tttgtataat tttgtcttgt
     2821 atcaattgat atggtgaaat acctttatac attctttctt cttttgcgga tactttttca
     2881 tattcctgtt ctgaaaataa tgtcattcat ctaatacgac caatttcttg atctaaattt
     2941 tcaaatacaa taagagcatt atattttttt gacatttgag taatataaga aactactttt
     3001 caaacaaatc atcctctgat tttagtactt gccaataaat ctttctctag aatctccatc
     3061 tcttcattag tgtgatcaaa tgcttgtaaa tgatatctaa ttttattagt tgcttttaag
     3121 tgttcaaatg cataattgta atcaaatact ttgtttcaat gattatcaac gattcaaagc
     3181 ttttctggtg ctatatcttc cagtattttt agcacttcaa ttaatgtttc ttttgacatt
     3241 tttgagataa cttggttttg gaatttttca gggaagtatc gccctaaagt gtatggttta
     3301 tttccttgtc ctcaaactaa taatttttgt tttcttttat aaggttgtca atcaatataa
     3361 caatcatcta attccacaaa gtctcatttg ttatttatca tagaaaggtc tttgataatc
     3421 ggcttacctt cttcatccaa aacaactctt ttttcattat caataagaca aatggtagct
     3481 aagctatttt ctcaaagatc aatacctatt gcatatgatt tatctacctc atccaagatt
     3541 ttcttttgca atttatcaag aggaagctct ttggttggat aaaatacaag ttgaaaatct
     3601 cagattattt tgttttcttt atatctcaat ttacttgttt cgtaattggg attatgatca
     3661 gaaatttctt caacaacttt agcaggacga acataaagat ttgattctgg atttaatcta
     3721 atattactag aattatctcc aaataaagat tcccagtata ttgtaaaaag attttgtttt
     3781 cccattatac ttatatcttt tttaatacga tttttatctc tttctgtttt agcaaaatat
     3841 ggatctacag caaaatcctt gttgtaaatc tgataaaagt ctaaaattca ttttttatca
     3901 agatcataaa ttatttgctt actaatactt tcccattttg ttatataaca ggttttagta
     3961 acatattttc taaactcagc aaagtcgtca aactcattgg atatattaaa tggtacccaa
     4021 ttaaaaactc atcattcaac ttttttatta agttgagtta ttgcttcatt caaaaaagaa
     4081 actagctttc taagttttgt tttttgatca ttggttatcg aacgagaatc ttcacttcct
     4141 ttctctttat aagttctttt ttctcggata tttttacaat ccctatattc tttatcatat
     4201 cgattaaaat ttagagcatt tttttcaaaa ataagtttct ctactgcctt agctgttagt
     4261 gaagagtatc tcaaaatctc aaaataatca tcgtttttta tctgattgtc tcataaatta
     4321 tctaattcat taatattatc cctatctatc attaccacaa aatatttttc tcagtttcta
     4381 acaagtcttg cgtaatgtga taatgcttgt tgagaataaa gttcttgctg ctttgcaaaa
     4441 aaatctcatt ttgctctacc atatatgaca ctagcatcaa tttttgctag tttaagttca
     4501 caatattctt tatctcaagt aaatttaggt tcgtactttt tttgttcttt ttttgctttt
     4561 gatctttctt ttagaatttt ttgataatta aaatctcatt cctttaatgt tttcaaatat
     4621 tcttttgctt gttttctttc attaggacta gctgtttgat tctctctaag tgctactaat
     4681 ttaggatcag tttttatttt ttcttgtaca aattttttgg tataatcatc aaaaatctgt
     4741 gtagctatta cttttttgtc ttctaaatca agcatttttg tttctagtat tctaatttct
     4801 ttttccattt tttctacatc tacttttttt ctaattgctt ttgcattaaa accaaatttt
     4861 gaaacctgga atcaacttgt tggactatat gattgtaaat atctatttgc gatgctaaaa
     4921 atatatcata aatctttatt atcataatat gattttaaca ttgtctttaa ctcattatct
     4981 gtatttcagg ttttataaat tatattagaa ataatttctt ctactttttg gacagcatga
     5041 acataccttt tgacagcaaa agctatttct gattttgtgg gttttttgtc tgtattgctt
     5101 ggtttttcta agctatctgc ccaaagtaag taagaaagac tatagttggt tgaatgtaat
     5161 ctatcaaatt tttctttgat ataatttgaa aattcttctt gaaattcttt actaagaact
     5221 actaatgtgt tatttgcact aagggcttta gattttaatc tgtaaaaata atctgagtct
     5281 attaacttta ttaaattttt atgtattact attttttgtg gaggaagatt tgataatatg
     5341 tttaatatct ctttttgttg tttgtgtatt ggtttcaatt tttcaaatag aacatgagga
     5401 tctgattcaa aactactatc aaaactacat ctttttaaag taaattcact aggtttgagt
     5461 tcaaatttaa tggttttaat aacctcatat aaatttgtta atttttccat cattgtattt
     5521 ttttaaaaaa ttaaaattct ttttgtgtaa aattatccca ttctacatta gtgattcata
     5581 aagttttttc attatcatta attttgttca aaatcattac tccttttcta gctatattgt
     5641 atgctccatt tgcatctcaa ttgaaatgtg ttccttgaaa accattatct gagtgaaatt
     5701 cacaagctgg acattgtaaa tatcctcaat catctcactc tttgctattt ctgatttgaa
     5761 gtaacaagtt tcgataaaac ataaagtctt taaatagttt aacatcagat ttttctagca
     5821 tcgattttac gatgttgttt tcatcaattc aatactcctt gaataagtca ctaaattttt
     5881 ctgaaagaac tatttctgta gatttaaatg ctgttcattt ttcaattctt tcattaaaca
     5941 atctaatttg attttttgta gtaatttttc gatcaagttt ttctctttta tttcctttct
     6001 tatcttctaa gctttctaat ttataaaaga aataaaaatc taatcatttt ttttcaattt
     6061 ttagatcaat gtcttttatc catttttgaa ttgatgccaa ggaatcatat tttttgtaaa
     6121 cattttttct aaatcagcac acaggacaag ttacagatgt atatcaagct tgagtataaa
     6181 ataatattca tgtttgtgca tagatgtctt ggaagttatc aactttagga gttaattgat
     6241 atgcattata aagtcatcac aattcattat cagctttatt tttatcaaca acaaaattta
     6301 attttttagc caaagctaac tcaaagtttt ggtaaatttg tctttctact ttttttctac
     6361 tatttttgaa acctgagttt agatcttcta atacaataat cgcgttgttt tgtatcgcta
     6421 gatcaacaat tttgtttact acatgcgaaa gatatcattg tttcatttct ttaatatttt
     6481 ctatttcgtt ccaatccacc ctttctttgg ctctatctca ttctttttta actaatttgt
     6541 cataataatc tacttctttt ttgcttcaat cagggagttt tgaagttaat atattcatac
     6601 tacataactg tttttcatca ccttgtttca aaatgtttca gttttgatca ataagtgcat
     6661 aatagagtaa atgtttttct cacctatcta ttccaataac attcattgta tgtttttttg
     6721 catattcttt aactttatca tttattttaa actcattctt attagtaaaa tttaatgtta
     6781 ttgggcaatg aaagagtatt ttattttctg taaatctttt attttcaatt gctccagtat
     6841 ttttcttttt caaatctttt tcttttttct ccgtaactgt cgctggacgg aagaatattt
     6901 ctgctcttca gttgagttta aaaaaaactc agttattaaa attttctttt tcaaataaag
     6961 ctttccagta ttttgtatgt agattttctt ttcatttacg atgttctgaa aaatctttat
     7021 tataaatttg gaatagatac aaatttcatt cttctacata tttgtgtaga gtgtttcagc
     7081 ttactgggac aaaagaaact ttatatcatt gattttctac atccgagtaa aattcattga
     7141 tttgtttata ttcagatgtg tctttaaaat aaaatcaaaa ttttgatcgg tctggatgaa
     7201 tacttaaaga tgatttcata aaatcaattc gtgtatgcaa atcttgcaac gaaaaatctt
     7261 ttcattgttt gaagctttcc ttctttctaa tattcaaaac cttgtcagat gggttgaaaa
     7321 aatcaatatt tttgtttgag aaaaaaactt ttggaagcat cttgttcggt catggcaaca
     7381 atttgtactc taatttttca tatttctctc attgatctga atataattcg tgatttgaat
     7441 ctttgaaaat tttattttct ccttttttca ttatagctaa aaaatactct ccctttcttc
     7501 tcaaaataac tcaaaagtta tcagattctt tatttttatc tcgtccatcg agaaaagtag
     7561 aattatcaaa atttaactta atcttatctg ttgaatattg tttttttgtg atatagtttc
     7621 ttacagcatt aaagaattta taaggtttga agtcatcaga ataaaattta tctatctcat
     7681 tgtaaaaatc agaatcaaat tccacatcaa ttagttcctt atcttttcat tgtcattttt
     7741 ccaatgaaaa atatcttaat atctgttcaa actgtaatac tatttggatt gtttctttta
     7801 taagtgtctt ggctgtcatc tttaagtctc agatttttat gtctttctct gaatttctaa
     7861 ggtttgtgga aaaattacca gagaataaat tcttttccaa aatatttgta taattttcta
     7921 agttttcttt ttgaatattg gcatccaata ctatagtttt taataattga ataaaagcat
     7981 tatctcaagc aatagaagta ttatactcct tcaaacaatc aaaccaatgt ttttttaaga
     8041 atcattcagt ctccgtagaa tttaatgcat ccttaatttc ttgtaacgaa atcacatcat
     8101 cacttttctt tcatttactt ttcttgtctg cattttcatc ttctggtaag agtgatcata
     8161 aaacatccca attatttaaa tacttattag aaatatcttt taaagctgaa cttttgaaat
     8221 aagttttttc gtaatcaaat ttttcttcca aaagtgcatt taagactgag tttattgatt
     8281 gctcaattct ttgtttagca tcctctagaa atgttttcaa attcttttcc aaatcttcat
     8341 catcttcgat aattgcattt acaaacgaaa aaatactttc tttgtcgggt tttgctaata
     8401 tttgtttatg taattttatt aacttaggaa gcttttctca tcacttatta gtttgattat
     8461 aagcattgat ttttgtgtta attcctcaaa ttatattatt atagatttca attcattttt
     8521 gatttagatg attttgaaaa cataaagcat caaagcaatt ttgttcatca gaatttagat
     8581 caataaatcc tacaaatttt tcataagatt ttttgttttg aaaaaatcta atcatatttt
     8641 catcaatgac tcttgttgca actctagatg ctttcccatc atctttataa agattatctc
     8701 tattatcttt gaaatttgaa agataagtaa aaaatcagac aaaataatca ttaataagct
     8761 ctttataggg ttttcaaatc aaatcaggat ttttgtattt tttactttta tctgttgtat
     8821 ctttagtata aatcctgtcc ccatagattt ctgccagaat tgggatgatt tctttttgtc
     8881 acaaaatctc gtatcatgaa atttttggat atttttttgc tattctagaa tgtttgagat
     8941 agtttacgat ctgttttctc aactcctttt ttgctttatc aaaatcatcc ttagatttct
     9001 ttttttcact ttcacttttg tgtctctcta ttgatcattt tcgcagatca taaacacttt
     9061 catctaattc caaagatgct cactccaaac tttcttcaat taattgagaa tgaagatcat
     9121 ttaaacacgg tttgatgatg ttttgataaa tttcgttaat tttcttatct tttggaaaag
     9181 gattctctca ttccaaaagt tcatttgttt ttcaaacagg tttcagttca aatctaagtg
     9241 tctttgaaag tggatagagg ttggtaaagt cagaaaagat atctttttta tcactcattt
     9301 tagaaaaatt acaaaataaa gattatctcc tatataccaa tttcaaaatt aaatacaaca
     9361 aagaaaaaat ctagcacaaa aaaatggcta atcaacatca gccattttat ctatattcaa
     9421 cataatttaa tttgggagga ctttgataga atccaaatca atttgacttg gttctaatgt
     9481 tatcatcctt cccttgtctg ttcttaagac aacttcttca tcacatatcc agacaatatg
     9541 tccttctgtg attgttccgt tgtaatccaa tacaactttt ctatcttcaa aattttttaa
     9601 ttgttcaata atttcactca taataattct ttttactcaa taattttaat ggactctatc
     9661 ataatgtcag gagtcaaaag ataaaggatc tctccagtat cttttcgttt gaaagtacat
     9721 tcaaaccgat caacattaat caattgtcct tccaacatta cgggtggaat aatcccgtca
     9781 ggtaggtggc catcataact aaggctaact accttccctt caaatttaat taatttttcc
     9841 ataattttag cttttaatta ctacttgatt ttacaacaaa cttactgatg tcacacataa
     9901 gttgtctagc acgacatttt gttcgcttgt ttctagtctt aatagacatc cataaaaacc
     9961 tacagaaaca agaacccctc ttagtttaag tggaggtttc atttctttcc atccacaatt
    10021 taagcatcca tcataagtta aggacacttt tttcccttca cattctttga gtttgttggt
    10081 taaatttccc ataaatttat atttttaatt aataattttg ttaacaaaac tccttatatg
    10141 tattttagat taaaagtaaa gtgatttttg aaatgttctt ttgaaaatgt ttttatatcg
    10201 catcaaaaac agtccaaact aaaaaatggc taactaaact tagccatttt tatctttttt
    10261 aaattttact caataacagt tattgattct agatcaattt tttctgtatc gaataaaaac
    10321 ttttttccat tttccagctt aaatacacac ttatggtcac caatgacagt aagtgttcct
    10381 ttcatggtaa atggtgctac gattccttct ggaatataat catcatttat atctccatga
    10441 taaactagaa ttaatttttt tctttcgaat gtttttaaaa atttcataat tacaattttt
    10501 ttgttaataa tattttgtta tcaaagtgat ttgtatatat ttaaaactaa aaataaagtc
    10561 ttctttataa tacgatatat cattttacaa aaaatggtgg tatttgttca catatattca
    10621 aatatttttg
//
LOCUS       DAOSID010000015        10059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_6511_length_10059_cov_16.457817, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000015 DAOSID010000000
VERSION     DAOSID010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 10059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10059
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6511_length_10059_cov_16.457817"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /gene="dnaB"
                     /locus_tag="PK674_01480"
     CDS             complement(<1..1301)
                     /gene="dnaB"
                     /locus_tag="PK674_01480"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="replicative DNA helicase"
                     /protein_id="HOG15235.1"
                     /translation="MVTINDIKLPPHNIEAEKGVLSGVLMDKNTLHIYNGDRLGPEDF
                     YQKEHQKIYEAILQLDDKTIDVVTLGNQLEKNGDLDFVGGLDYLYELSTFLLSTSGCS
                     EYSKIVKDKSLLRQILKTSQNIIGNVYKQENTTNILESIEKNIFDLTQSQTGNTIKHI
                     NEIMGGRTKEYMAIVDNPEKVNSTKVFSGYKKLDNMLTGFKPGELIILAARPSMGKTS
                     FALNIVTNAALQQNKSIAIFSLEMGSESIVDRIIAEVAQIPMKKITKGDLTNEDFSKI
                     GEAMTILGDKKIYIDDKSATNIPLLKSKLRRLKVEKGELDLVIIDYLQLMHGTNFLGN
                     RVQEISEISRGLKELARELEIPIIALSQLSRAVEQRIDKKPHLSDLRESGAIEQDADS
                     VIMLHREDYYDKDTDKKGLTDVCVRKNRNGEVGEIELYFKA"
     gene            complement(1648..2487)
                     /locus_tag="PK674_01485"
     CDS             complement(1648..2487)
                     /locus_tag="PK674_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOG15236.1"
                     /translation="MNKKISLVFMVAGLSSRFGGKIKQFAKVGPNDSTLIEYSMDQAL
                     KSNFSEIIFIVGKMTEQAFKEKFGDNYKGIPVKYAKQSFDENERDRPWGTVDAVCSAK
                     DLINNPFVVCNGDDIYGENSFKILYDHLQNSDEMATLGYILGNVIPEQGTTNRGIFGI
                     TEDNYVDNITEMIGISKDTLSDFGLKKDDLCSMNIFGLNKKTLNQLDNILTNFKLENK
                     GNRKIECYLPVELSNIIKIEKNKMKIYSTPDRRLGITNPEDEGIVKKQIEEIEKNKNV
                     ENL"
     gene            complement(2511..3821)
                     /locus_tag="PK674_01490"
     CDS             complement(2511..3821)
                     /locus_tag="PK674_01490"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG15237.1"
                     /translation="MIAEYYKLLKEYLGFKSVSTNNNFDQDIKNCSNRLKELFEQNNF
                     QVEVVNKYGNPIIIASYFVDNNLENGLIYGHYDVQNAKQIDGRKENPFNLYIGKDKVI
                     GRGVVDNKGQTLVHILSIIKLIKENKLGYNITFILEGEEEIGSPGIMKFIEENKQKFK
                     TDFILISDRSIVKNLPTIGSGFRGGFNAKLEIKTANTDLHTGIYGGVVLNPIEELINL
                     FSKLYDANNQVTIPYFYYEVEELSVNQKILNSKIPFNQKDLNTNLGISQTKLKDIDFY
                     TKSGWLPCVEITGFNSGNTEFFKNSIPNTATANINFRLVNNQKCDSIVNLFKERIKNN
                     LPKNIEYKLNFEQYSKPTKINLQNKFIDKAEKLLTDIYANKVNYLRSGGSLPIIDIFQ
                     TYISNNILSIPLANEDSNMHGVNENFDIDLIKKGLEFSYKFFQK"
     gene            complement(3846..4598)
                     /locus_tag="PK674_01495"
     CDS             complement(3846..4598)
                     /locus_tag="PK674_01495"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG15238.1"
                     /translation="MKNELLQQRAKETREENNINDEGIISSIFENKNPETESKEKLGT
                     IKENSEENTNKNILEKQKKILEKISYEANVYKKDGKLEEYEKKLIEGLAIEPDNLEFN
                     KQLADLYFTIGNHKKALSLLKKIIEEDPDDHNAIRQIGEIYLISGDYETAELLIEKAI
                     NTKPSNPKYYISMVEILYNTERKKDAIAVMEKIVKLRPTNSNYALTLAELYEETQDND
                     NAKKYYFKVLEQEPNNEKAKRKIQELSNSNIE"
     gene            complement(4797..>5243)
                     /locus_tag="PK674_01500"
     CDS             complement(4797..>5243)
                     /locus_tag="PK674_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15239.1"
                     /translation="DMVKMDPRTDILPCGADGIIPMHCKTTLPLPSIEMENLILQNLE
                     GRDIERLKGRCGKLKCSLIYELEIYREESKKYPPKGSEVKYLKGNIDGLVTSYNIMTG
                     DVTIKTQDGETFKIPTGELKITKLPDPKIKTEEEKELEKIKSEIEG"
     assembly_gap    5244..5343
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5344..5872)
                     /locus_tag="PK674_01505"
     CDS             complement(<5344..5872)
                     /locus_tag="PK674_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15240.1"
                     /translation="MNKAKNDQSKNSVYVLDRYTNKNIKVINIDETSLQELKTGDKLV
                     YNMEENNQKPSIGIYIGHDINTDRIGHFTKILKGEELDFFEEQSKFALQIFPLFKKRF
                     KAEFKNSIPVTARFHIYLDQLYFYFYSEERYIFTDFVKKLREKIGKNIFLFQVGARDM
                     VKMDPRTDILPCGADG"
     gene            complement(5899..7770)
                     /gene="ftsH"
                     /locus_tag="PK674_01510"
     CDS             complement(5899..7770)
                     /gene="ftsH"
                     /locus_tag="PK674_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOG15241.1"
                     /translation="MFKKTKNVRISILVLLLLVIIYQTYQSTNIDKNIIQTIKKQEEV
                     SLNLFIDKYTHKEFSKIDVINDSKLIGYVFIGTGEGKSFMTIQKTLTEEIYNVFESKK
                     PENTSLIDLGIELDGSTEINIKSEQESFLTSILKEVVPLLIFFVLILFLFRFSLPKGG
                     GLPFNTKAGKENIKTKENTKFSDVAGMEEVKEELSEIVDYLKNSSKYQKVGARHPKGV
                     LLYGQPGSGKTLLARAVAGEASVPFFSVSGSEFMEMLVGMGAAKVRNLFQKAKTAGKA
                     IIFIDEIDAIGRKRGLGHTGGHQEQEQTLNQILTEMDGFDNKTNIIVMAATNRPDILD
                     PALLRAGRFDRKILVSAPTYEERKEIFEYYLKGKKVEKNLNLDSLIKRTSGLVGADIE
                     NIVNEAALKLAKENKTVIGEEEFEYALEKVLMGPEKKVKSMQEKEKQIIAYHELGHAV
                     TSHLLENTDKVEKITIVRRGQALGATWKTPEEDKYLYSKNKIIDETISLLGGRAAEEV
                     FFGKDEITTGASNDLERATKIITDMVMKYGMDEELGPVFYFDGSREEYLPYKTYSEKV
                     AEKIDEKIKLYINNCYLESKKIITKNKKLIESMSKVLLEKEYLTREEFVELMEDSIK"
     gene            8051..>10059
                     /locus_tag="PK674_01515"
     CDS             8051..>10059
                     /locus_tag="PK674_01515"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOG15242.1"
                     /translation="MMSLKKQLKTNDRYLKILQKNGINNLKEFFQYFPRDYENRENLV
                     TLDQINILDKAICSVKGFITDKRFFQRGARKTFTIKFNDEKGNRGEIVIYNSAFLASK
                     IKVNSWYIIVGKPYFKYGKFSFSNPEILETEAIDDNVDQQNDPSFGRIYPIYSELNGI
                     KPSWFAKKMWDILPEIETTFSEYLPQDFLQEFNLIDIKTTIKNLHYPNNIDNVRQGKY
                     RIFFDKLLRLQLHSIINRNEYRQNDIDLNGSQEDRAIVKFIVDRLEFQLTGAQKKVIK
                     KVIEDIHSGKPMMRLLQGDVGSGKTVVAAIVAFYVYKKFGAQSVFLAPLEVLANQHYK
                     TLAKLLLPLGLRIEILKGSLTQGQKDKVKSDLKDGKIHILVGTHAVLQDDVEFKDLKF
                     AIIDEQHKFGVKQRAVFHKFANPHILQMSATPIPRSMALAFFGEFDVSVIDEMPKGRK
                     EIYTKIISESEFVKLKPWILTRIMQKQKIFIVTPLIEESEKMENLKAATVEFENIKSL
                     FFEIKDKIGLLHGKMKSTEKDEVMKGFQGDKYDILVSTTVIEVGVDVPQATVMLIKNA
                     ERFGLSQLHQLRGRIGRSDLQSYCFLEAKNKSGDSYKRLKAMEETNDGFKLAELDLES
                     RGAGEILGTMQSGQTDLPLEILMNMKFVEKVREGAEWLLEHYPGL"
BASE COUNT         3086 a         1360 c         1360 g         4153 t
ORIGIN      
        1 cttgctttga aatacaattc tatttctcaa acttctcagt ttctgttttt tcttacacaa
       61 acatctgtta atcatttttt atctgtgtct ttgtcataat aatcctctct atgtaacatt
      121 ataacactat ctgcgtcttg ttcaatagct catgattctc ttagatcgct tagatgtggt
      181 tttttatcta ttctttgttc tacagcacga gataattgtg aaagggctat aattggaatc
      241 tctagctctc tggctaattc ctttaatcat ctagaaattt cactgatttc ttggactcta
      301 tttcataaaa agtttgtacc gtgcatcaat tgtaggtaat caataattac caaatccaat
      361 tctcattttt ctacttttaa tctcctaagt tttgatttta ataaaggtat atttgttgca
      421 gatttgtcat ctatataaat ttttttgtct cacaaaattg tcatagcttc tcatatttta
      481 gaaaaatctt cattggttaa atctcctttt gtgatttttt tcattggtat ttgtgctact
      541 tctgctataa ttctatcaac aatggattct gaccccattt ctagtgaaaa tattgctatt
      601 gatttgtttt gttgtaatgc agcgttggtg actatattta atgcaaaaga tgttttaccc
      661 attgatggtc tggctgcaag tattatgagt tctcatggtt tgaaaccagt taacatatta
      721 tctaattttt tataaccaga aaaaactttt gtgctgttaa ctttctctgg attatctacg
      781 atggccatgt attcttttgt tcttcatcac ataatttcat ttatatgttt tatagtattc
      841 cctgtttgac tttgtgttag gtcaaatatg tttttttcta tagattctag aatgtttgta
      901 gtgttttctt gcttataaac atttcaaatt atgttttgtg aagtttttaa tatttgtctt
      961 aaaagagatt tgtcttttac tattttggaa tattcagaac atcatgaggt agataataaa
     1021 aaagttgata gttcataaag atagtctaat cctcccacaa aatctaaatc tcaatttttt
     1081 tctaattggt ttcaaagagt tactacatct atagttttat catctaattg taaaattgct
     1141 tcgtatattt tttgatgttc tttttggtag aaatcttcag gtcatagcct gtctcagttg
     1201 taaatatgta atgtattttt gtccattaaa actcatgaca aaactcattt ttctgcttca
     1261 atgttgtgtg gaggtagttt tatatcgttg atagtgacca ttttgtagaa aggttatgga
     1321 gttaaaagtt ataaagtagt ttaaaagttt ataaagtttg ttgagttttt acaatttggg
     1381 aaggtggggg tttgtggttt ttagatcccc cttagtcccc cttattaagg gggattccac
     1441 caccccaact accccaacta cactagaaca gcttccccct agggaagaac ttttcactag
     1501 taaagtttat aaaattataa agttcataaa gttacgagtt tgtaaagttt atcaagttat
     1561 aaatatgtga taaaaatctt atattaaaaa aaagataata ttcaagagaa aatgtagttt
     1621 tcaattagct ttttatcaac atagatttta caaattttcc acatttttgt ttttttctat
     1681 ttcttcgatt tgttttttta ctattccttc atcttcagga ttagttattc acaatcgtct
     1741 atctggagtt gaataaattt tcattttgtt tttttctatt tttattatat tagatagttc
     1801 tacaggtaaa tagcactcaa tctttctgtt ccctttattt tctagtttga aatttgttag
     1861 tatattatct aattgattta aagttttttt gtttaatcaa aagatattca tgctacaaag
     1921 atcatctttt tttaatcaaa aatcagacaa tgtgtctttt gatattcaaa tcatttctgt
     1981 tatattgtca acatagttgt cttctgtgat tccaaatatt catctattgg tggttcattg
     2041 ttctggaatt acatttcata gtatgtatca taatgttgcc atttcatctg aattttgtaa
     2101 gtgatcgtat aatatcttaa atgagttttc tcaatatata tcatctcaat tacaaacaac
     2161 aaacggatta tttatcaaat cttttgcact acaaacagca tctactgttc cccaaggtct
     2221 atctctttca ttttcatcaa aactttgttt agcatatttt actggaattc atttataatt
     2281 atctcaaaac ttttctttga atgcttgttc tgtcattttg cctacgataa atataatttc
     2341 tgaaaaattt gattttaaag cttgatccat tgagtattca attaatgtag aatcattggg
     2401 tcatactttt gcaaattgtt ttatttttca accaaaacga gatgataatc atgctaccat
     2461 aaatacaaga gaaatttttt tattcatagt cttatattta gaaattaaaa ttacttttgg
     2521 aaaaatttat agctaaattc tagtcatttt ttgatcaaat ctatatcaaa attttcatta
     2581 actcaatgca tattagagtc ttcatttgct aatggtatag atagtatgtt attactaata
     2641 tatgtttgaa agatatcaat aattggtaaa gatcatcatg atctaagata atttactttg
     2701 ttagcatata tgtctgttaa tagtttttct gctttatcta taaatttatt ttgtaaattt
     2761 atttttgttg gtttactata ttgctcaaaa ttcagcttat attctatgtt ttttggtaaa
     2821 ttatttttaa tccgttcctt aaataaatta actatagagt cacatttttg gttgtttacc
     2881 aatctaaagt taatatttgc tgttgcagta tttggtatag aatttttaaa aaattctgtg
     2941 tttcatgaat tgaaccccgt aatttcgacg catggtagcc atcaagattt ggtataaaaa
     3001 tcaatatctt ttaattttgt ttgagaaatt caaaggtttg tatttaagtc tttttgatta
     3061 aagggtattt tgctatttaa tattttttga tttacactca gctcttctac ttcataataa
     3121 aaatatggta tagtcacttg attgtttgca tcgtataatt tggaaaataa gtttattagt
     3181 tcttcaattg gatttaggac tactccacca taaattcagg tgtgtaaatc tgtgtttgct
     3241 gtttttattt ctaattttgc attaaatcat catctaaatc atgagccaat ggttggtaag
     3301 ttttttacaa tacttcgatc tgatattaat ataaaatcgg ttttaaattt ttgtttattt
     3361 tcttctataa atttcataat tcatggagag cctatttctt cttctcattc caagataaat
     3421 gtaatattgt atcacaattt attttctttt ataagtttta ttatgctcaa tatgtgtacc
     3481 aatgtttgtc atttattgtc tactactccc cttcagataa ctttatcttt cccaatataa
     3541 agattgaacg ggttttcttt tcgtcaatct atttgcttag cattttgaac atcataatgt
     3601 ccataaatta atcaattttc taaattatta tccacaaaat aagatgcgat aattatgggg
     3661 tttcaatatt tatttactac ttctacttga aaattatttt gttcaaatag ttcctttaat
     3721 cggtttgaac aattttttat atcttgatca aaattattgt ttgtagaaac acttttaaat
     3781 cataaatatt cttttaataa tttataatat tctgctatca tatcgttcgt tatttgagat
     3841 aaattttatt ctatattact atttgataac tcttggattt ttctttttgc tttttcgttg
     3901 tttggttctt gttccaatac tttaaaatag tattttttag cattatcatt atcctgtgtt
     3961 tcttcgtata attcagctag agttaaagca taatttgaat tagttggtct taattttaca
     4021 attttttcca ttactgctat tgcatccttt tttctttcag tattgtataa gatttcaacc
     4081 atactaatgt aatacttagg attacttggt tttgtattaa ttgctttttc tattaataat
     4141 tctgcagttt cataatctca actaattaaa taaatttctc atatttgtcg tatcgcgttg
     4201 tgatcatctg gatcttcttc tattattttc tttaataaag ataatgcttt tttatgattt
     4261 catattgtaa aatataagtc agctagttgt ttgttgaact ctaagttatc tggttctata
     4321 gccaaacctt ctattaattt tttttcatat tcttctaatt taccatcttt tttatataca
     4381 ttagcctcat aagatatctt ttctaatatc tttttttgtt tttctaaaat atttttattt
     4441 gtattctcct cactattttc ttttatagtt cacagttttt ctttggattc tgtttcagga
     4501 tttttatttt caaaaatact acttattatt ccctcatcat tgatgttgtt ttcttctctt
     4561 gtttcttttg ctctttgttg taataattca ttcttcattt tatctaaagt tttgaggtta
     4621 tgaatttttt gttttattaa agttatcatt ttatttcatc ttttagttaa aaaaattacc
     4681 actattctaa ttgggaaaat aagtaaaatt acggtaagta ctatccaaag agttgtccct
     4741 agtttagtca taaaaccaat tttaaaattt aaaaatgcga aatataaggt tgtttattat
     4801 cattctattt cactttttat tttttctaat tctttttctt cttctgtttt tattttagga
     4861 tcaggtaact ttgttatttt caactctcaa gtaggaattt taaatgtttc tccatcttga
     4921 gtttttattg ttacatcacc tgtcattatg ttgtatgatg ttactagtca atcaatgttt
     4981 cattttaaat attttacttc acttcatttt ggaggatatt ttttactttc ttctctatat
     5041 atttctaatt cataaattaa agaacatttt aatttaccac atcttcattt caacctttct
     5101 atgtctcttc attctagatt ttgaagtatt aaattttcca tttctatact aggaagaggt
     5161 agtgttgttt tacagtgcat gggtataatt caatcagctc cacaaggtag tatatctgtt
     5221 ctaggatcca tttttaccat atcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnttcaatc agctccacaa ggtagtatat ctgttctagg atccattttt accatatccc
     5401 tagcacctac ttggaataaa aaaatatttt ttcaaatttt ttctcttaat tttttcacga
     5461 aatcagtaaa tatatatctt tcttctgagt aaaaataaaa gtatagctga tctaagtata
     5521 tatgaaatct agctgtaaca ggtatggaat ttttgaattc tgccttaaat ctttttttaa
     5581 ataaaggaaa tatctgtaat gcaaatttac tttgctcttc aaaaaaatcc aactcctctc
     5641 attttagaat ttttgtaaaa tgtcatattc tatcagtatt tatatcatgt caaatataaa
     5701 ttcatataga gggtttttgg ttattttctt ccatgttgta gacaagcttg tctcatgttt
     5761 ttagttcttg tagtgaggtt tcatctatat ttataacttt tatatttttg tttgtgtatc
     5821 gatctaaaac atatacggaa tttttagatt gatcgttttt tgctttgttc ataataattt
     5881 ttagaattaa aataattttt attttatact atcttccatt agttcaacga attcttctct
     5941 ggttaagtat tctttttcca gcaaaacttt tgacatagat tctattagtt ttttgttttt
     6001 tgtgattatt ttttttgatt ctaagtaaca attgttgatg tacaatttaa ttttttcatc
     6061 tattttttca gcaacttttt cactgtatgt tttgtatggt aaatattcct ctcttgatcc
     6121 atcaaaataa aatacaggtc ataattcctc gtccattcaa tatttcataa ccatatcagt
     6181 tatgattttt gtcgctcttt caagatcatt tgaagctcat gtcgtgattt catcctttcc
     6241 aaagaatacc tcttctgctg ctcttcatca taataaagat attgtttcgt caataatttt
     6301 gttttttgag taaagatatt tgtcttcttc tggtgttttc caggttgctc ataatgcctg
     6361 tcatctacga acgatagtta ttttttctac tttatctgta ttttccaata aatgtgatgt
     6421 aacagcgtgt cataattcat gatatgctat tatttgtttt tctttttctt gcatgctttt
     6481 tacctttttt tctggtcaca ttaagacttt ttctaaagca tattcaaact cttcttcacc
     6541 tattactgtt ttgttttctt ttgctaactt taatgctgct tcatttacta tattttctat
     6601 gtcagctcaa acaagaccac tagttctttt tattagagaa tcaagattta gatttttttc
     6661 tactttttta ccttttaggt aatattcaaa tatttccttt ctttcttcat aagtaggagc
     6721 agaaaccaat atttttctat caaatctacc cgctctcaat aaggctggat ctagtatgtc
     6781 aggtctgttg gtagctgcca ttacgatgat atttgttttg ttatcaaatc aatccatttc
     6841 tgtgaggatt tgatttaagg tttgttcttg ttcttggtgt catccagtat gtcaaagtcc
     6901 tctttttctt caaatagcat ctatttcatc tataaatata attgcttttc atgctgtttt
     6961 tgctttttga aaaaggtttc taactttggc tgctcacatt caaactaaca tttccataaa
     7021 ctctgatcat gatactgaaa agaatggaac agaagcttct catgcaacag ctcttgccaa
     7081 caaggttttt caagatcaag gttgtcagta taataaaact cattttggat gtctggctca
     7141 cactttttga tattttgatg agttttttag ataatctact atttctgata attcttcttt
     7201 tacttcttcc attcaggcaa catctgaaaa ttttgtattt tctttggttt ttatgttttc
     7261 ctttcaagct tttgtattaa aaggtagtca tcatcatttt ggtagagaaa acctgaataa
     7321 aaacaatata agtacaaaga atatgagtag cggtactact tctttgagaa tcgaagtcaa
     7381 aaaactttct tgttctgatt ttatgtttat ttctgtggat cagtctagtt caattcataa
     7441 atctatcaaa gatgtgtttt ctggtttttt agattcaaaa acattgtaaa tttcctctgt
     7501 taatgttttt tgtattgtca taaaagattt tccctctcca gttcaaataa aaacatatcc
     7561 aattaactta ctgtcgttta ttacatctat tttagaaaac tctttgtgtg tgtatttatc
     7621 tatgaataaa tttaatgaaa cttcctcttg ttttttgatt gtttggatta tgtttttatc
     7681 aatgtttgta gattgatagg tttgatatat gattactaat agcagtagaa ctagaattga
     7741 tattcgtaca ttttttgttt ttttaaacat tttttatcta atatgacaaa aataaaaaaa
     7801 gttgaatttt tattcattta gaatatataa aaattggttg gaatttcaaa tagaaaacat
     7861 tattaaatta atagtgaaag tttataaagt aacatgtgag ttaaaagtta taaagttgaa
     7921 agttgcaagt tacaagttta taaaattgta ttctcctacc acccgggtgc aattggctag
     7981 cttcgcgaac agcacccggg tggtaggata tgaacttgac aaactttcaa acctttttac
     8041 aaaatgaatg atgatgagtc tgaagaaaca gcttaaaact aacgatagat atcttaaaat
     8101 acttcaaaaa aattgaataa ataatttgaa ggagtttttt cagtattttc ctagagatta
     8161 tgaaaatagg gaaaaccttg ttactttgga tcagattaat attttggata aagcaatttg
     8221 ttctgtaaaa tgatttataa ctgataaaag attttttcaa agatgagcaa gaaaaacttt
     8281 tactataaaa tttaatgatg aaaaatgaaa tagatgagaa atagtgattt ataattctgc
     8341 ttttctggct tccaaaataa aagtaaatag ttggtatata atcgttggta aaccatattt
     8401 taagtatggt aaatttagtt tttccaatcc tgaaatttta gaaacagagg ctattgatga
     8461 taatgttgac caacaaaacg atccttcttt tggaaggata tatcctatat attcagaatt
     8521 gaattgaatt aagccttctt ggtttgctaa aaaaatgtgg gatattttgc cggaaatcga
     8581 aactactttt agtgaatact tacctcaaga ttttttacaa gaattcaatt tgattgatat
     8641 aaagactaca ataaaaaacc tacattatcc aaacaatata gacaatgtta gacaaggaaa
     8701 atataggatc ttttttgata aattacttag attgcagtta cactctatta taaatagaaa
     8761 tgagtatagg caaaatgata tagatttgaa tggaagtcaa gaagaccggg ctatagtaaa
     8821 atttatagtg gatagattgg agtttcaact tactggtgct caaaaaaaag ttataaaaaa
     8881 agttatagaa gatattcatt cttgaaaacc aatgatgagg cttttgcaat gagatgttgg
     8941 tagctgaaaa accgttgttg cggctatagt tgctttttat gtatataaga aattttgagc
     9001 tcaatctgtt tttcttgctc cattagaggt tttagcaaat cagcattata aaactttagc
     9061 taaattattg cttccattat gacttcgtat tgagattttg aaatgatctt taacacaatg
     9121 acaaaaagat aaagttaaat cagatttgaa agattgaaaa attcatattt tggtatgaac
     9181 tcatgctgtc ttacaagatg atgtggagtt taaggatttg aagtttgcaa ttatagatga
     9241 acaacataag tttggagtta agcaaagggc tgttttccat aaatttgcta atcctcatat
     9301 tttacaaatg tctgctactc caattccaag atcaatggct ttagcatttt ttggagaatt
     9361 tgatgttagt gtgattgatg aaatgccaaa atgaagaaaa gaaatttata caaaaataat
     9421 ttctgaatca gaatttgtga aattaaaacc atggatattg actagaatta tgcaaaaaca
     9481 aaagattttt atagtgacac cattgataga agaatcagaa aaaatggaaa atttaaaagc
     9541 ggcaacggta gagtttgaaa atattaaatc attatttttt gaaataaaag ataagatatg
     9601 acttttacat ggtaaaatga aatccacaga aaaggatgaa gtgatgaagg gtttccaatg
     9661 agataaatat gatattttag tttcaacaac tgtgatagaa gtttgagtag atgttcctca
     9721 agcaacggtg atgcttataa aaaatgctga aaggttttga ctttctcagc ttcatcaact
     9781 aaggtgaaga atatgaagaa gtgatttaca atcatattgt tttttagaag ctaaaaacaa
     9841 atcttgagat tcttacaaga gattgaaagc tatggaagaa acaaatgact gattcaaact
     9901 agcagaacta gatttagaaa gtcgtggtgc ttgagaaata ttatgaacga tgcaatcgtg
     9961 acaaacagat ttgcctttgg aaatattgat gaatatgaaa ttcgtagaaa aggttagaga
    10021 atgagcagaa tggttattgg aacattatcc atgacttga
//
LOCUS       DAOSID010000016         9810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_6685_length_9810_cov_13.995284, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000016 DAOSID010000000
VERSION     DAOSID010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9810
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6685_length_9810_cov_13.995284"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PK674_01520"
     CDS             <1..287
                     /locus_tag="PK674_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15243.1"
                     /translation="VKNIFDSTFKGIVLLDYKFLQQEKIILFLNLYSPEEVDRDDIYD
                     TLLSDLKLEFSDEIELIIQWQINGNNKEETTELDSLSSGQVDLSGSLESL"
     gene            775..1695
                     /locus_tag="PK674_01525"
     CDS             775..1695
                     /locus_tag="PK674_01525"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOG15244.1"
                     /translation="MKKFIVFLKGILMGTCDVIPGVSGGTIAFITGIYDKLIDSLYGF
                     NLSTLKLVFKGKFKEAWNAVNGSFLLTLFAGILVAVLTFAKLISFLLINHPSYVWAFF
                     FGLVLSSVVILIKSLKSGFKVIYLLWLIIGVVIGFMLTSLPVVNLGTSNLIIFGSGAI
                     AIMAMILPGISGSYILLILGQYQNILQYVVNLTSRDWSAIIPLLIFILGCLIGLILFA
                     KLLYRVKKKWHNQMVLVLTGFIIGSLQKIWPWKETIQTYLDSHGIEQPLVQTNIIPSW
                     SMDVIWCVALAILGFLIVWIIDIISKKNER"
     gene            complement(1836..2489)
                     /locus_tag="PK674_01530"
     CDS             complement(1836..2489)
                     /locus_tag="PK674_01530"
                     /inference="COORDINATES: protein motif:HMM:NF020366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DcrB-related protein"
                     /protein_id="HOG15245.1"
                     /translation="MQKKLILPLLILFLILQGCMFKENNFLNNTDQQTDQTENINNIE
                     NSQNTDNIKNVDDAKNVENTSDSTETAQYENKIENFSLDFPKNWTVKENTFNVTISTP
                     KDDEINENVIIVTQQLQKFLSVAEYYEETIRQLKETLENFKETKSKDVTQGELKGKTI
                     IYEHIHKDTNLTLKSLQTFFMAPENRVYSINYTATKDSFDKYIEGVETIINSFKLNK"
     gene            complement(2573..3316)
                     /locus_tag="PK674_01535"
     CDS             complement(2573..3316)
                     /locus_tag="PK674_01535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG15246.1"
                     /translation="MKIKKDLENFYNQEAEKYYFTRKKYRSDGYIILEEIKKNPKQTI
                     SILEFGCGSGRFISFLNQNLQDKKINYIGIDLSQKLIEFAKKDNSNNKFIQDDISNYI
                     KQTKQESFDYIVGIASFQHIPSKKERLFLLKSFYRSLNYGGKLIMTNWSISKRFIKKY
                     YKVFINSLLKFLYTSGNYSRNDLYIPWKNKGITNKRYYHIFTKKEIKNLTKTAGFKTI
                     LLTYVNNKGQKIKNRKKSNNTIFIGEKNI"
     gene            complement(3319..4434)
                     /locus_tag="PK674_01540"
     CDS             complement(3319..4434)
                     /locus_tag="PK674_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOG15247.1"
                     /translation="MFFSFEKDYYINIKNIIYKIITNNYHNIHTFKTYKTIMYKKFSL
                     EEIEKIKNNFIPHLYSIVKKERCQGINDLVKHIFKEGNSVYFFERGEPFFKIAINNNI
                     LEFTDEIERIQLFLAMEEKGKLQSLLKTYIVQKEKQTGQKTIEQILYDEFKTGNYSYI
                     LDKPYMVFDIETTMGVGNNLDSYKFLLAYAMRPGDEKMSYHYIDINNLNDFAKTLTEF
                     EGYIIGFNNFAFDNPISIKQGGFGEKELGIINKKSLDIFQFIRNISGKRIGLNKLSET
                     LIGIQKTLESGTKGELLWKQYQETGDKKHLEELKKYCKNDVRMTAFVLLYLLHFKKIF
                     IEGEEITFNIEDFVKLAKPKGDIQNEDDNKFSNQSIF"
     gene            4511..4729
                     /locus_tag="PK674_01545"
     CDS             4511..4729
                     /locus_tag="PK674_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15248.1"
                     /translation="MKKKLLSVLVLFLATLLVAGCVNNPVIEDDLGGNDVIEDDVIID
                     EEIMPEPEEEIIEEEIVEEEIVEDVFVE"
     gene            4938..5630
                     /locus_tag="PK674_01550"
     CDS             4938..5630
                     /locus_tag="PK674_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="pseudouridine synthase"
                     /protein_id="HOG15249.1"
                     /translation="MDIRLNKYVQENLNLSRRAFVSLVQAGKVFLNDKIVESYSQTVK
                     IGDFLEIKGKGIKEKIKDLNKNLDVILFNKPVGFVCSKSDRHNKTIYEILPKKFHNYF
                     YIGRLDKDSRGLLILTNDSVLVDKLQHPKNNVEKEYLVQIDRAFSVNDYKKMKKGILD
                     EGDLLTVKTAKFFEDKKKFFIKIILGEGKKRHVRRMLTSLGYKILDLQRVREAQFKLG
                     NLKEGNWIRVNI"
     gene            5669..6067
                     /locus_tag="PK674_01555"
     CDS             5669..6067
                     /locus_tag="PK674_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15250.1"
                     /translation="MFKSKFRLKGKDINFLTRKKRYFSTTFFGFFYFEQYPNLKFNQI
                     SVNIPLKYNKKAVCRTSLKRAILRFLKENGFEKKDINGKFYKIFISVNKSNIGELKNK
                     IERFDKATTNNYIVEQFQKSFLFFLSKIGK"
     gene            6058..7413
                     /locus_tag="PK674_01560"
     CDS             6058..7413
                     /locus_tag="PK674_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="AI-2E family transporter"
                     /protein_id="HOG15251.1"
                     /translation="MKVKSNFWANLKRVFSFRTQNEKTSNQDVEEVKLLEKIKNKQTE
                     SKNHSCWDNRQMFRFWLFGLVVVILGYFAYNTLNLIFLFISAFILSMIVESLISRFQA
                     KKIKRSVSIGLSYFIFILVLLVLFLVFIPFLTVQTVELVSLGLNYLAELQKDITTLGV
                     DQIIMNFKFLPSSFKEYLLSSFNSNFIEQFQLVLQEHLSEIISFGRLYITKAGTIMIS
                     FVSGFTKFLINFGTFIILAIFFSIDKDLITKFLAKIGGKDNYKINCLKIKKMYKRLGV
                     WFKARLLMSIIVALVVWLALVVLGWFGIYVPNKLGISLFIGLLDIVPYVGAFISGLIL
                     FAVSTLYNPFIASLLVVGIYVVLNMILSNVFLPIFMNKALGVNASLIFIGMIFGGMIM
                     GFFGVLLAVPIVVIIILLFEDKEQLNKKEEDFNISKIVSKSAHKIIKKMPHKQKNSEK
                     K"
     gene            7441..8841
                     /locus_tag="PK674_01565"
     CDS             7441..8841
                     /locus_tag="PK674_01565"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="Mur ligase family protein"
                     /protein_id="HOG15252.1"
                     /translation="MKKKIFNFYCKLLAFLAKNYLKKHKVFVIGVNGSVGKTTARMII
                     YQTLSKFINNKKIYTSSKNFNGELGLSLSIFKIEEFEPSFFCSIKTFFQTIIKLLFSR
                     QPYDIIILEYGIDRPLEMEFLLEIIKPNIGVFTAIDSVHSLQFGSPNEIAKEEKKMIE
                     NTVEFAFLNVDDGYAMSLIKNLEIDYLTYQTEGYDSTGDISFENEKFSYEENKLLVNF
                     DLKIKEKNINVTTNLIGKAHYGYIGVSLAILDIINYREGKQSIFEKFEDIFLDYKLQA
                     GRLSVFDGVDGSIIFDSTYNSSPLSVKKILNTVHNIKKDLFSDRDLRIILGDMRELGD
                     LTESDHRKVAGYVYSLADKLFLVGESMNNYLKDELEKIGFDMSLIYSFDDSIALGNFV
                     KQELKRDKRKKLIIAKGSQNTIFLEEAVKILLKNPEDSDKLTRQSSWWKNKKKAWFHK
                     GVGGLGITHPGAVFGW"
     gene            8953..9582
                     /gene="rplI"
                     /locus_tag="PK674_01570"
     CDS             8953..9582
                     /gene="rplI"
                     /locus_tag="PK674_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L9"
                     /protein_id="HOG15253.1"
                     /translation="MPKRSLTNRTIEVILLESDKHLGEKFEIVRVKPIYARNVLLPKN
                     IAVLATADMVNKYSQKMESAKKEREKRAAGFQGLLQNIEKDGGLVFTGKVNKDANLYA
                     KIDASNIAELIKSVYSVDVEEHFFKMKKKITTIGDYVVPFIYKDIKGDISVKVLAENP
                     EDIEKKSKAKHTETVEAEEKVEDKKEDKKSEDIKEEESEEKKVEKTMAD"
BASE COUNT         3389 a          991 c         1492 g         3938 t
ORIGIN      
        1 aagtgaaaaa tatttttgat agtacattta aatgaattgt tttattggat tataagttct
       61 tacaacaaga aaaaattata ttattcttaa acttatattc tcctgaagaa gtagatagag
      121 atgatatata tgatacttta ttatctgatt tgaaattaga attttctgat gagatagaat
      181 tgataattca atggcaaatt aatgggaata acaaggagga aactactgag ttggatagct
      241 tgtctagttg acaagtagat ttatcagggt cattggagag tttataaagt tagaaagcct
      301 gcctgccatg tagccaatac tgcggggcgt ggcggagcgg ggtttgtaaa gttagaaagt
      361 tacaagctta tcccgcccat actcttgtag ttttatagag cttgcaggat ttggcggagc
      421 tggggaaacg aactccattc tatgaaagga gacagttttg ttgtttttcc tactggctta
      481 aatttccata gcggaaagca agcaacttcc tccaaagaag ggaggcagtt ttgttgttag
      541 taatttccat tttaattgta gttccgcgat tctgcggggc ggggctgcaa gaagtgggtt
      601 gtgaaatatt cgttaacaga taattggatt ctgcaaataa attcttcgta acaataatgc
      661 tcagaaagac aatgatgtag ctaaaattaa ggcaggatta tgaaaatcta taaacacttg
      721 aaaaaaaata acagcttctt attatttaaa agcttttatt ttttttatta aaatatgaaa
      781 aaatttatag tatttttaaa gtgaattttg atgtgaactt gtgatgtgat accatgagtt
      841 agttgatgaa caatcgcttt tattacttga atatatgata aacttattga ttctttatat
      901 tgattcaact tatccacatt aaaattagtt tttaaatgaa agtttaaaga agcttggaat
      961 gcagtaaatt gatctttttt gttaacatta tttgcatgaa tactagttgc tgtattaact
     1021 tttgcgaaac ttattagttt tttactgatc aaccatcctt cttatgtttg ggcgttcttt
     1081 ttcggattag tgctttcatc cgtagttatt ttaattaaat ccttaaaaag ctgattcaaa
     1141 gttatttatt tattgtggtt gattattggt gttgtaatat gatttatgct gacatcatta
     1201 ccagttgtaa atttatgaac atccaatttg attattttcg gttcatgagc aatagcaatt
     1261 atggcaatga ttttaccttg aataagtggt tcttatattt tgttgattct tggacaatat
     1321 caaaatatct tacaatatgt tgtaaatctt acttctcgtg attggtctgc gattatccct
     1381 ttgttgatat ttattttatg atgtttaatt tgattgattt tatttgctaa acttctttat
     1441 cgagtaaaga aaaaatggca taatcaaatg gttttggttt taacatgatt tattatctga
     1501 tctttacaaa aaatttggcc atggaaggaa acaatacaaa catatttgga ttctcatgga
     1561 atagaacaac cattagttca aactaatatt atcccttctt ggtcgatgga tgttatttgg
     1621 tgtgttgctt tagctattct tggtttccta attgtttgga taattgatat aatttcaaaa
     1681 aaaaatgaga ggtagtttta ttgatagatt atttggcaaa aaatacaaaa aaatataata
     1741 atatattata ataaaagttt ttttatcgtg ctttttttat ttttaattga tttgtcttaa
     1801 atttatctgt gtttttggtt ttttgcaata attttttatt tatttaattt gaaggagtta
     1861 attattgttt caactcattc tatatattta tcaaaactgt cttttgttgc tgtataattg
     1921 atggagtaaa cacgattttc tggtgccata aaaaaggttt gtagagattt tagtgttagg
     1981 tttgtgtctt tatggatatg ttcataaatt attgtttttc ctttcagttc tcattgagtt
     2041 acatctttac tttttgtttc tttgaaattt tccaaagttt cttttagttg tcgaatcgtt
     2101 tcttcgtagt attcggccac acttaaaaac ttttgtaatt gttgagtaac tataataaca
     2161 ttttcgttta tttcatcgtc ctttggggtt gatattgtta cattaaatgt attttctttt
     2221 accgtccaat tttttggaaa atctagtgaa aaattttcta ttttattttc atattgagca
     2281 gtttctgtgg aatcgctggt attctcgaca ttcttagcat catcaacatt tttaatatta
     2341 tcagtatttt gagaattttc gatgttgttg atattttctg tttggtctgt ttgttgatcg
     2401 gtattgttta aaaaattgtt ttctttaaac atacatcatt gtaaaattaa aaataagatt
     2461 agaagtggta atattagctt tttttgcatt ttagtttttt tatataaata aatatttgtt
     2521 tgttaccaag ataataaata tttaattttg tcctaatttc aacccattat tgttaaatat
     2581 ttttttctca tataaaaata gtgttgttag atttttttcg gttttttatt ttttgtcact
     2641 tgttgtttac atatgttaat agtatagttt taaatccagc tgtttttgtt agatttttta
     2701 tttctttttt tgtaaagatg tgataatatc ttttattagt tattccctta tttttccaag
     2761 ggatatataa gtcgtttcga ctgtaatttc aagatgtata tagaaattta agtaaagaat
     2821 ttataaaaac tttatagtat ttttttataa atcgttttga gatagaccaa tttgtcatta
     2881 tcagttttca tcaataattt aggcttctgt aaaagctttt taacaaaaac aatctttctt
     2941 ttttgcttgg gatgtgttga aaactcgcta ttcatacaat gtaatcaaag gattcttgtt
     3001 ttgtttgttt gatataatta gatatgtcat cttgtatgaa tttattatta gaattatctt
     3061 tttttgcaaa ttctattaac ttttgtgata aatctattca aatgtaattt atttttttat
     3121 cttgtaggtt ttgatttaaa aaagatataa atcttccaga tcaacatcaa aattctaaaa
     3181 ttgagattgt ctgtttaggg ttttttttaa tttcttccaa aattatgtat caatcagatc
     3241 gatatttttt tctggtgaaa taatattttt cggcttcttg attgtagaag ttttccaaat
     3301 ctttttttat tttcattatt aaaaaattga ttgattgcta aatttgttgt catcctcatt
     3361 ttggatatct cattttggtt tagctaattt cacaaaatct tcaatattaa aagttatctc
     3421 ttctcattct ataaatattt ttttgaaatg taacaaatat aataggacaa aagcagtcat
     3481 tctaacatca tttttacaat attttttgag ttcttctaga tgttttttat ctcaggtttc
     3541 ttggtattgt ttccataaaa gttctcattt agttcctgat tctagtgttt tttgtattca
     3601 tataagagtt tcagataact tgtttagtca tattcttttt ccggatatgt ttcgtataaa
     3661 ttggaatatg tccaaacttt ttttgtttat gattcccagt tctttttcac caaatcctca
     3721 ttgttttatg ctaattggat tgtcaaaagc aaaattgtta aatcatatta tatatcattc
     3781 aaattccgta agagttttag caaaatcgtt taaattgttt atatctatgt aatgataact
     3841 cattttttca tctcatggtc tcatagcata ggctaataaa aatttataag agtccaaatt
     3901 gttccccact cacattgtag tttcaatatc aaataccata taaggtttgt ctaatatata
     3961 actgtaattt catgttttaa attcgtcata caatatttgt tcgatagttt tttgtcatgt
     4021 ttgtttttct ttttgaacaa tgtatgtttt caataaactt tgtagttttc atttttcttc
     4081 cattgcaaga aataattgaa ttctttcaat ttcgtcagta aattctaaaa tattattatt
     4141 tatagcaatt ttgaaaaatg gttctcatct ttcaaaaaaa tatacgctat ttcattcttt
     4201 aaaaatatgt tttactaaat cgttgattca ttggcatctt tcttttttta ctatggaata
     4261 tagatgtggt ataaaattat tttttatttt ttctatctct tctaatgaaa attttttgta
     4321 catgatcgtt ttatatgttt taaaagtatg gatattgtgg taattatttg ttattatttt
     4381 ataaattata ttctttatat taatataata atctttttca aaagaaaaaa acattttttt
     4441 ctttttctct tgaaatattt gtttttttat ttatacacac cgtatgattt tattttataa
     4501 atttttgggt atgaaaaaaa aattattaag tgttctagtt ttgtttctag ctacattact
     4561 tgttgcttga tgtgttaaca atcctgttat tgaggatgat ttaggaggaa atgatgttat
     4621 tgaagatgat gttattattg acgaagaaat tatgcctgag cctgaagaag aaattataga
     4681 agaagaaatt gtagaagagg aaattgttga agatgttttc gttgaatagt gtggatagta
     4741 aaattagaga tgtttttgta agataacatc ttgaaataag ttattaaaaa gttaaaaaaa
     4801 agggtgattt tatcattact ctttttttgt tgtacctttt tttgatttta tatatagaat
     4861 ttataaaaag tactagaatt gattattatt accatgtatt tgcgtaaaag gtgactattt
     4921 tatttcattt tttgaatatg gatattcgtt tgaataaata tgtacaagaa aatttaaatt
     4981 tatctagaag agcttttgtt tctttagttc aagcatgaaa ggttttttta aatgacaaga
     5041 ttgttgagtc ttattctcag actgttaaaa tatgagactt tttagaaatt aaatgaaaat
     5101 gaataaaaga aaaaataaag gatttaaata aaaacttgga tgtaatattg ttcaataaac
     5161 cggttgggtt tgtttgttca aaatctgata gacataataa aactatatat gagattttac
     5221 caaaaaagtt tcacaattat ttttatattt gaagattgga taaggatagt agaggattgt
     5281 tgattctaac caacgattca gttttagtag acaagttgca acatcctaaa aataatgtag
     5341 agaaagaata tttagttcaa attgatagag ctttttctgt gaatgattat aaaaaaatga
     5401 aaaaatgaat tttagacgaa tgagatttac ttacagtcaa aacagccaag ttttttgagg
     5461 acaaaaagaa attttttatt aaaataatac tttgagagtg aaaaaaaaga catgttcgta
     5521 gaatgttgac atcattatga tataaaatat tggatttaca aagggtgaga gaagctcaat
     5581 tcaagttggg aaatctgaag gaatgaaatt ggataagggt taatatttag ttcgtatatt
     5641 attataatat aatcagtaaa taaacaaaat gtttaaaagt aaatttagat taaaatgaaa
     5701 ggatattaat tttttaacca gaaaaaaaag gtatttttct acaacttttt tttgattttt
     5761 ttattttgag caatatccaa atttaaaatt taatcaaatt tctgttaata ttccattgaa
     5821 atataataaa aaagctgtat gtagaacctc tctcaaaaga gctattttaa gatttttaaa
     5881 agaaaattga tttgagaaaa aagatattaa ttgaaaattt tataaaattt ttattagtgt
     5941 gaataaaagc aatatatgag aattaaaaaa taaaattgaa agatttgaca aagctaccac
     6001 aaataattat attgtagaac agtttcaaaa aagtttttta ttttttttga gtaaaatatg
     6061 aaagtaaaat ctaatttctg ggctaatttg aaaagagtgt tttcttttag aacacaaaat
     6121 gaaaaaacaa gcaatcaaga tgttgaggaa gttaaacttt tggaaaaaat taaaaataag
     6181 cagacagaat ccaaaaatca ttcttgttgg gacaataggc aaatgtttag gttttggtta
     6241 ttttgattag tagttgtgat actatgatac tttgcttaca atacattaaa ccttattttt
     6301 ttgttcatct ctgcttttat cttatcaatg atagtggagt cgcttatttc tcgatttcaa
     6361 gctaaaaaga taaagagatc agtttctatt tgattatctt attttatatt tattttggtt
     6421 ttattggtgt tatttttagt ttttatacca ttcctaaccg ttcaaacggt tgaattggtc
     6481 tctttgtgat taaattattt agcagaactt caaaaagata ttactacatt gtgagtagat
     6541 caaataatta tgaattttaa atttttgccc agctctttta aagaatattt actttcttct
     6601 tttaattcta actttattga acagtttcaa cttgtgttac aagagcattt gtctgaaatt
     6661 atttcatttt gaaggttata tattacaaaa gcgggtacta taatgatatc atttgtttct
     6721 ggatttacaa aatttttaat taatttttga acatttatta ttttggctat tttcttttca
     6781 attgataagg atttaataac caaatttcta gctaaaatat gatgaaaaga taactataag
     6841 ataaattgtt taaaaataaa aaaaatgtat aaaagattgt gagtttggtt taaggctaga
     6901 ttattaatga gcataattgt agctttagtt gtttggcttg cattagttgt tttatgatgg
     6961 tttgggatat atgtaccaaa taaattatga atatctttat ttatatgatt attggatata
     7021 gtcccttatg tttgagcatt tatttctgga ttaattttgt ttgctgtatc tacgctttat
     7081 aatccattta tagcatcttt attagtagta tgaatttatg tagttttaaa tatgatttta
     7141 tcgaatgttt ttttgccaat ttttatgaac aaggcattat gagttaatgc aagtttaata
     7201 tttattggta tgattttttg aggtatgatt atgtgatttt ttggtgtttt attagccgtt
     7261 cctatagtag ttattataat attgttgttt gaagacaaag aacagttaaa taaaaaagag
     7321 gaagatttta acatctcaaa aattgtttca aaatctgcac ataaaattat taaaaaaatg
     7381 cctcataagc aaaaaaacag cgaaaaaaaa taaattgtct tttaatatgt atttgaaata
     7441 atgaaaaaaa agattttcaa tttttactgt aaattactgg cttttttggc taaaaattat
     7501 cttaaaaaac ataaagtatt tgtgatatga gttaattgaa gtgtgggtaa gacaacagct
     7561 agaatgataa tttaccaaac tttatctaaa tttattaaca ataaaaaaat atatacttct
     7621 tcgaaaaatt tcaattgaga attgtgatta tctctttcta tatttaaaat agaagaattt
     7681 gaaccttctt tcttttgttc tataaaaacc tttttccaaa ctataattaa actcttgttt
     7741 tctaggcaac cgtatgatat aataattttg gaatatggaa tagatcgtcc tttggaaatg
     7801 gaatttttat tagaaattat aaagccaaat attggtgtat ttacagcaat agattccgtt
     7861 cattctttac agtttggtag tcctaatgaa attgctaaag aagaaaaaaa gatgatagaa
     7921 aataccgtag agtttgcatt tttgaatgta gatgactgat atgcaatgtc tttgataaaa
     7981 aatttagaaa tcgattattt aacatatcag acagaatgat atgacagtac tggagatata
     8041 agttttgaaa atgaaaagtt ttcctatgaa gaaaataaat tgttagttaa ttttgatcta
     8101 aaaattaagg aaaaaaatat aaatgttacg actaatttaa tatgaaaagc tcattatggc
     8161 tatatttgag tttctttggc gatattagat attataaact atagggaatg aaaacaatct
     8221 atttttgaga aatttgaaga tattttctta gattacaagt tgcaagcagg tagattatct
     8281 gtttttgatg gtgttgatgg ttctatcata tttgattcta catataattc ttctcctttg
     8341 agtgtaaaaa aaatattaaa tactgttcat aacataaaaa aagatttatt ttctgataga
     8401 gatttgcgaa ttattttgtg agatatgaga gagttatgag acttgacaga atcagatcac
     8461 agaaaagtag ctggatatgt gtattcatta gcagataaat tgtttttagt ttgagaaagt
     8521 atgaataatt atctaaaaga tgaattagaa aagatttgat ttgatatgtc actaatttat
     8581 tcttttgatg attcaattgc tctgtgaaat tttgttaaac aagaattaaa aagagataaa
     8641 aggaaaaaat taattattgc taaggggagt caaaatacaa tatttttgga ggaagctgtt
     8701 aaaattcttt tgaaaaatcc agaagattca gataaactta ccagacaatc aagttggtgg
     8761 aaaaacaaga aaaaagcttg gtttcataag ggagtaggag ggttgggtat tacccacccg
     8821 ggtgctgttt tcgggtggta gcatcttcca actttataaa ttttttaatt ataattgata
     8881 tattttgttg caatttgttt tcaaattctt atataattaa ctaattctat tttagatatt
     8941 aatatttttt tgatgccaaa aagaagttta acaaatagaa ctattgaagt aattttattg
     9001 gaatcagaca aacatctctg agaaaaattt gaaatcgtga gagtaaagcc tatttatgct
     9061 agaaatgttt tattgccaaa aaatatagct gttcttgcta cagctgatat ggtaaataaa
     9121 tattcacaaa aaatggagtc tgccaaaaag gaaagagaaa aaagggcggc atgatttcaa
     9181 tgacttttgc aaaatattga aaaagattga ggtttggtat ttactggaaa agtaaataaa
     9241 gatgctaatt tgtatgcaaa aattgatgca tcaaatattg ctgaattaat aaaatcagta
     9301 tattctgtgg atgtagaaga gcatttcttt aaaatgaaaa agaaaattac taccatatga
     9361 gattatgtag ttccatttat ttacaaagat atcaaatgag atatttctgt taaagtttta
     9421 gcagaaaatc cagaagatat agaaaagaaa tctaaagcta aacatactga aacagtagaa
     9481 gctgaagaaa aagttgaaga taaaaaagaa gataaaaagt ctgaagacat aaaagaagaa
     9541 gagtccgaag aaaaaaaggt tgagaaaaca atggcagatt aagaaatact attacaacta
     9601 atatatgaaa aaatcttcat ttactttggg atttttttta aaataaatat taattttttt
     9661 agagttaaga gtttttttct attgagaatt ttttttcaat ttttatacta tattttgttt
     9721 agttgattat taaaaatatg tttgatttaa gatcacagtt taaaccagct ggagatcagc
     9781 caaaagcaat aaaagaaatt attgatggtt
//
LOCUS       DAOSID010000017         9736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_6747_length_9736_cov_11.302758, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000017 DAOSID010000000
VERSION     DAOSID010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 9736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9736
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6747_length_9736_cov_11.302758"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PK674_01575"
     CDS             complement(<1..574)
                     /locus_tag="PK674_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15254.1"
                     /translation="MFKNKGNQVNNNENKGNIAFQNFGNNFKSSINWFRQNKDKVKFL
                     IPIAIITSVLMIYMIIKTFINISNLNKESKELYQIKNFNIKVLENNDYTKEEIKSKKT
                     LNDLINYEIELSKEIEKYNSYLSILQSPYSNFMKYIFLPELNIWKDPFTNEIDTDLIG
                     IKYLEKNPYNDIKLIQKRSDFIKNVGANNEF"
     gene            complement(600..1952)
                     /locus_tag="PK674_01580"
     CDS             complement(600..1952)
                     /locus_tag="PK674_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15255.1"
                     /translation="MTENKNINLGEILNNSNIENFSTNENIIPQINKNNNLDNNKDTI
                     NLENIKIEEAKSFSNEKIIFENIELENKEKKHYRINYRKIFIISFLTIIITVSVSIGL
                     KTYNNYINDQVTNIENKENKIISNINKIKDFLKTYLGLDTKHGAGNNYNLIGEDGINN
                     LNEIIKSKINYIQKKDILSTAVKNLNNNILSNHKTLENLKKSISKEGFLSKEIGEIIS
                     SEEQISSIYNSLLSLESIKFSSAINVFPYLDTFIEGLSNASKISKKEIEEKNKIIISR
                     GEKDINLYLKNCYLNPFESDYECSTIGDFEQYYNLNDDKTFDTSFFKSLIKYTDTKLE
                     QSELPSFSIDFKKFDKNSNKISFNIDINTFKQDELELAKKGILSPHVFILNNLINNLK
                     QSKFILGRGISVKTLKTEAKTIEIGSTEFTINNSNKSFTVEIQKESEREIDDFISTNN
                     "
     gene            complement(2110..3114)
                     /locus_tag="PK674_01585"
     CDS             complement(2110..3114)
                     /locus_tag="PK674_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOG15256.1"
                     /translation="MYNLYQSNLRKDIQTKIYNKPHFTINLFGKDYFGLVKEKNIGPF
                     KTKWLQIMGVEFPTDKDYIKSELKKLAQKYKKEKGVIFVQLCLLNEIIRFENVGIRSD
                     SFLNDMKEIRLQVRQQMKELYGLETAFRENMPQANIVYDITKSDEELMSEMNSGCKER
                     VKKAIKKGIEFGVASPDQYDLFYQKWIELSGMKGFNIIPKEQYHKLIRYMTQNNRGNL
                     FTSTINGDIIAGSICVYDQHRIIYLYGFSNRKYSNIGGHHYLKFKIFGHARKLGMTYV
                     DMLGGAPTGFPTHPLNGVSRFKESLGGTKIEQYGSYDLIIRPRLYKLFKFYFKLKNRE
                     "
     gene            complement(3182..4510)
                     /locus_tag="PK674_01590"
     CDS             complement(3182..4510)
                     /locus_tag="PK674_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15257.1"
                     /translation="MKKIYKLFLYILIGITGIMGISQANFDLIISKIYLSNGGDTVAL
                     YSEPSINIKIENIGVGTAVNDGPISEGFIKCIEVNSSLEVFLSSTMTKFIIEPDSGMI
                     ARNLQLSDNLTETQREVKIKCSINEDGARNNSFTSNETDTSNNTKEFSFRVDKLSRFD
                     PSMNRAIEPIKDNLDAAEPSSTIGGGDSIRSFIFNKIVNVVTPFIIIIGVMVGIIGAY
                     KLFFSDKEEDLKKGVMLVIYGIVGIIIILSARYIGSVIFEDILQSGDTVGLNGADIAQ
                     QLYGKIAYPFIKIVLYLALGILFLALAGKTLGFITQSDGSGQKKAITMIARSAISMLI
                     IIGAKQLVEAVYGKQDNVLNQSAQNLGDIGGGILANKNIPIIYTIINRIMGLTALVVL
                     IIIIFQTFQILVNPEKSENRQKLGKTILYIFIGILIIGTGYVITNFLIIN"
     gene            complement(4512..5456)
                     /locus_tag="PK674_01595"
     CDS             complement(4512..5456)
                     /locus_tag="PK674_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15258.1"
                     /translation="MNQKQNTYSNKTELALSALMFFSPLIQNLTKKNDINKSEQNFLK
                     GYIKLGYFNIILLGMTIFFQIIYYTSNNSLFQNLGFVISIVLGISLTVGSICAILNKE
                     IFGNNNKTNLTHSKDALLDNILCYIPIYNIYKRYDEHKFENPNILLKESLLLWTIFSI
                     LLLIFPGESLIIMIILIVVLRIISVSNGVYGGEKFENTINKIFKKNPEEIWGYFLGII
                     KTLFNGKKIKENISNIKQTFELIPKIDYKQILGQYLILLIILLFSFYLGIKNDNINVV
                     VALLLITGRYGVMIIKRKHVPNIPILKEFVNLFFKKKS"
     gene            complement(5458..7515)
                     /locus_tag="PK674_01600"
     CDS             complement(5458..7515)
                     /locus_tag="PK674_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF87 domain-containing protein"
                     /protein_id="HOG15259.1"
                     /translation="MKQKLKNIVFDTNHTRQVKEGGVIDFPISNNDNKTQEDKKENKK
                     VAKKGFLDRLIESIIPQTKGASNQKNGEEENPILKNLSLKEQVRQIIEMPDEEYSDKI
                     LTNQLRQYISAAEKQYYKTISDYKSHMAPSFWETNSTYFNVAGLLGRSYYVQSYPSYI
                     DALRTREIFSFDTKRDMSFILYPKDDAAIQSMLKQKATQLKAEISESIRKGITLDTEI
                     EQQYRDVEEIRQKLTTREERYFELGYYTNIYEEKEDKLKETGRKFEQKISGYGVKVKH
                     SIQRMDEGFNSILPLCNDELGITRSAVTSSLAGSFPFISNDLIDKTGILYGINSYTGS
                     LVIFDRFNGKLPNMNSCILATSGAGKSFTVKLEILRYLINGIDTIVIDPENEYKGLCD
                     RVGGTYVNIATNSQQHLNPFDIPPNIEDVEYGPGDLLRSQIMNLIGLIELLIGDLSTE
                     EEALLDKALQSTYNLKGFSFEDTKYEGKQPPIMEDLMNTLDGMDGGEKMALKLSKYVT
                     GTFGKVFNNYTNVDITNKMTVFSIRDLEEALKTPAMFNVLNFIRTKVRAQKKKRLLVC
                     DEARIMLKHKTSAEFLFGLIKRARKYGVGITTISQDIEDFTRSEYGKPIISNSALQIL
                     LKQSTTSIKLLNSLLGLSEVEQRRLVSCGIGEGLIFAGNQHVGVKILASAEEAAFIST
                     DVK"
     gene            complement(7699..8733)
                     /locus_tag="PK674_01605"
     CDS             complement(7699..8733)
                     /locus_tag="PK674_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15260.1"
                     /translation="MNYELFKHKNKGIFLVDMRRETDYIFSEITKASLEKQIKNNKKI
                     GIIVNKKGFSSGIFCQKCGYVPKCNRCDVNISYYKINENKIGLCNICKTQYNFPLECP
                     HCHSAEIKEFGIGTQRIAEYIQTEYKVKTVIIDADTTRSQKKIKEILEQKDIQIYIGT
                     SLLNTPIKGVELDLIIFLNADIGLNIPDYSAAKNNFHFLYDTFTSHTCKNYIVQTFNP
                     EVYSIRNACKMDKELFTIEDNQFRKKNLYPPFSDVCVISYKDEIEEKLFNKIDIMYKD
                     LLYLKDKYQMDNLEIYTTPPLIYKMFNKYRYNIILKGESLRNFMDIVYTKLRINQKHF
                     KVDRNTENII"
     gene            complement(8989..>9736)
                     /locus_tag="PK674_01610"
     CDS             complement(8989..>9736)
                     /locus_tag="PK674_01610"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=25
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOG15261.1"
                     /translation="GCPANNVMKSGGGSALLKNKESTLNIIKTLSKNLKVPFSIKSRT
                     GLNEEDKKKQMDFLTEASNFCHMITIHARTLKELYSGDGDREFIYELKKKANKNCKII
                     GNGAIKNFNEITNKIQNLDGVMIGQSAIGNPRIFTNHSPTIQEKFETILEHINLLAKF
                     EIFTSDLNPKNLGKNKIKPKLQEIESIDIQSNYEKIYYTPLLFRTYLHQYLKGIPGSK
                     ELKEICNQTKDFNQTIKNIEQFFKATSLSN"
BASE COUNT         3041 a         1271 c         1184 g         4240 t
ORIGIN      
        1 taaattcatt atttgctcaa acatttttaa taaaatcact tcgtttttga attaatttta
       61 tatcattgta aggatttttt tctaaatatt ttataccgat taaatcagta tctatttcat
      121 ttgtaaaagg atctttccaa atgtttaatt ctggtaaaaa aatgtatttc ataaaattag
      181 aataaggact ttgtaaaata gatagataag aattatattt ttctatttct tttgataatt
      241 ctatttcata atttattaaa tcatttaatg tttttttaga tttaatttct tcttttgtat
      301 aatcattatt ttctagaact tttatattaa agttttttat ttgataaagt tctttagatt
      361 ctttgtttag attactaatg tttataaaag tttttattat catataaatc attaaaacac
      421 tagtgatgat agcaataggt atcaaaaatt ttactttatc tttgttttgc ctaaaccaat
      481 tgatactaga tttgaaatta ttgccaaaat tttggaaagc tatgtttcat ttattttcgt
      541 tattatttac ttgatttcat ttgtttttaa acattatatt ttttattatg ataaataaac
      601 taattatttg tacttataaa atcatcaatt tctctttcac tttctttttg tatttctact
      661 gtaaatgatt tatttgaatt gttgattgta aattctgttg atcctatttc tattgttttt
      721 gcttcagttt ttaatgtttt tacagatatt cctcttccta aaataaattt actttgtttt
      781 aagttattta ttaaattgtt taaaataaac acatgaggag ataatattcc ttttttagct
      841 aactcaagtt catcttgttt gaatgtattt atgtctatat taaaagaaat tttattacta
      901 tttttatcaa attttttaaa atctatgcta aaacttggaa gttcagattg ttctaatttt
      961 gtgtctgtgt attttattaa acttttaaaa aaacttgtat caaaagtttt atcatcattt
     1021 agattataat attgttcaaa atctcaaata gtactacatt cataatcact ttcaaatgga
     1081 tttaaataac aatttttaag atataaattt atatcttttt ctcaccttga tataattatt
     1141 ttattttttt cttctatttc ttttttagat attttgctag cattagataa tcattcaata
     1201 aatgtatcta aatagggaaa aacatttatg gcactactaa actttataga ctccaaagag
     1261 agtagtgagt tgtaaattga agatatttgt tcttcacttg atataatctc tcaaatttct
     1321 tttgataaaa atcattcttt actaatagat ttttttagat tctctagtgt tttatgatta
     1381 gacaaaatgt tgttgttcaa gttttttacg gctgtactta atatatcttt tttttgtata
     1441 tagtttattt tagattttat tatttcattt agattgttga ttcaatcttc tcctataaga
     1501 ttataattgt tccccgctca atgtttagta tctaatccta aataagtttt taaaaaatct
     1561 ttaattttat ttatatttga tattattttg ttttccttgt tttctatatt agtcacttga
     1621 tcatttatat aattgttgta agttttcaat catatagaaa cacttactgt aataattatt
     1681 gttaaaaatg atataataaa aatttttcga taattaatgc gataatgttt tttttcttta
     1741 ttttctaatt caatattttc aaaaataatt ttttcattac taaagctttt ggcttcctct
     1801 atttttatat tttctaaatt aattgtatct ttattgttgt ctaaattgtt gtttttgttt
     1861 atttgaggta ttatgttttc attagtagaa aaattttcaa tattactatt gtttaagatt
     1921 tctcataaat ttatgttctt gttttcagtc atattatcat attagaataa atataatata
     1981 cactattatt atactcaaaa agagaaaaaa tatgaaaaaa aagagattta tttgctcttt
     2041 tctttttgac ataagtatct attggtgtgt ggtatgaggt gagctgtgtt tataataaaa
     2101 tatttgtttt tattctctgt tttttaattt aaaataaaat ttaaataact tatataatcg
     2161 tggtctaatt atgagatcat aactgccgta ttgttctatt tttgttcatc acaaggattc
     2221 tttaaatcta cttactccat tgagtgggtg agttggaaat cctgttgggg ctcatccaag
     2281 catatctaca taagtcattc atagttttct agcatgtcca aaaattttaa actttagata
     2341 atgatgacct cctatattag aatattttct atttgaaaat ccatataaat aaattatacg
     2401 atgttggtcg tatacacaga tactcccagc tataatatct cagtttattg ttgaagtaaa
     2461 taaattacca cggttgtttt gagtcatata tctaatcaat ttgtggtatt gttcttttgg
     2521 tataatatta aaacctttca ttcaagataa ttctatccat ttttgataaa ataagtcata
     2581 ttgatcagga gatgctacac caaattcaat tccttttttt atagcctttt ttactctttc
     2641 tttgcaacca gaattcatct cactcattag ttcttcatct gattttgtaa tgtcgtatac
     2701 aatattggct tgtggcatat tttctctaaa agctgtttct aatcagtata attctttcat
     2761 ctgttgacga acttgtagcc taatttcttt catatcattt aaaaagctgt cggatcttat
     2821 tcaaacattt tcaaatctta ttatttcatt gagtagacat agttgaacaa aaattactca
     2881 cttttctttt ttgtattttt gtgctaattt ttttagttct gattttatat aatctttatc
     2941 ggtagggaat tcaactcaca taatttgtaa ccattttgtt ttgaatggtc atatattttt
     3001 ttcttttact aatcaaaaat aatctttacc aaataaattt attgtaaaat gtggtttgtt
     3061 gtatattttt gtttgtatat cttttcgtaa attactttgg tataagttgt acactattgg
     3121 atatttgttg ataaataaat cgtagtgtag tatataattt tttgcggaaa aagcaagact
     3181 attagttgat tattaggaaa tttgtaatca cataacctgt tcagattatc aatattcata
     3241 tgaaaatata taatatagtt ttgccaagtt tttgtcgatt ttcagatttt tctggattta
     3301 ctagtatttg gaaagtttga aatattatta ttattaatac aaccaatgcg gtaagcccca
     3361 taattcggtt tattattgtg tatatgatgg ggatgttttt attagctaat attcatcatc
     3421 atatatctca tagattttga gcactttgat ttaaaacatt atcttgtttt ccatacacag
     3481 cttccactaa ttgtttagct catattatga ttaacataga tatagcactt cgtgctatca
     3541 ttgttattgc ttttttttga ccagatcaat cagattgtgt aataaatcat agtgttttac
     3601 ctgctaatgc taaaaacaga attcataaag ccagatataa tactattttt ataaatggat
     3661 atgcgatttt cccatataat tgttgtgcta tatctgctca gtttagtcat acagtatctc
     3721 aagattgtaa tatatcttca aatattacac ttcatatata tctagcactt aaaattataa
     3781 ttattcatac aattcaataa attaccagca taactcattt ttttaaatct tcttccttat
     3841 ctgaaaagaa taatttatat gctcatatta ttcataccat tacaccaata attattataa
     3901 aaggagttac tacatttact attttattaa atataaagct tcttatggag tctcaccctc
     3961 ctatggtaga actgggttca gctgcgtcta aattgtcttt tatgggttca attgctctgt
     4021 tcatagaagg atcaaatcta gatagtttat ctactcgaaa agagaattct tttgtattat
     4081 tacttgtatc agtttcatta ctagtaaagc tattatttcg agctcaatct tcatttatac
     4141 tacattttat tttcacttct ctttgtgttt ccgttaaatt atccgacaat tgtagatttc
     4201 ttgctatcat tcagctatct ggctctatga taaatttggt cattgtagat gatagaaaca
     4261 cctctaatga agagtttact tctatacact ttataaatcc ttctgatata ggtcaatcat
     4321 taaccgccgt tcctactcat atattttcta tttttatatt gattgagggt tctgagtata
     4381 aagctaccgt gtcacctcca ttacttaaat aaattttact tattattaaa tcaaaatttg
     4441 cttgtgaaat tcacataatt catgttattc atatgagtat gtacaaaaat aatttgtata
     4501 tttttttcat tttagctttt tttcttaaaa aataaattta caaattcttt taaaattggt
     4561 atatttggaa catgttttcg tttaattatc attacaccat atcttcatgt aattaataac
     4621 aaagcaacaa ccacattaat gttatcgttt tttattcaca aataaaatga aaatagtagt
     4681 attattaata atattaaata ttgtcacaat atttgtttgt aatctatttt tggtatcaat
     4741 tcaaaagttt gtttgatatt agaaatgttt tcttttattt tttttcaatt aaatagtgtt
     4801 ttaataattc ctaaaaaata tccccagatt tcttctggat tttttttgaa tattttattt
     4861 attgtatttt caaatttttc tcatcaataa actccgtttg atacggatat tatccttaaa
     4921 acaacaataa gaataatcat aattatcaaa ctttctcaag gaaaaattaa taaaagtata
     4981 ctaaatatcg tccataataa caaggattct tttaacaata tatttggatt ttcaaatttg
     5041 tgttcatcat atcgtttata gatgttatat attggtatat aacacaaaat attatctaat
     5101 agtgcatctt ttgagtgagt taagtttgtt ttattattat ttcagaaaat ttctttgttt
     5161 aatattgcac aaatacttca aactgtcaaa gatattcata aaactatact tataacaaat
     5221 cataaatttt gaaataaact gttgttgctt gtgtagtaaa tgatttgaaa gaatatagtc
     5281 atccccaaca gtattatatt aaaatatcct aatttaatat atcctttcaa aaaattttgt
     5341 tctgatttgt ttatatcgtt ttttttggtt aagttttgta ttaatggaga aaagaacatt
     5401 aatgcagata atgctaattc tgttttatta ctatatgtgt tttgtttttg attcatatta
     5461 ttttacatct gttgatataa atgccgcttc ttctgcagag gctaatattt ttactcaaac
     5521 atgttgattt caagcaaata ttaatcattc tcatattccg caggatacca accttctttg
     5581 ttccacttct gataatcata ataaactatt taataatttt atagaggtgg tagattgctt
     5641 taataatatt tgtaaagctg agttggatat gataggttta ccatattcgc ttcttgtaaa
     5701 atcttcgata tcttgagata ttgttgtaat ccctactcaa tactttctag ctcttttaat
     5761 taatcaaaac agaaactcgg ctgaggtttt atgttttaac ataatccgtg cttcatcaca
     5821 aactaatagt ctttttttct tttgtgctct tacttttgtc cgaataaaat ttaatacatt
     5881 aaacatggca ggagttttta atgcttcttc tagatctctt atactaaaaa cagtcatttt
     5941 attggtgata tctacatttg tatagttgtt gaatactttt ccaaaagtac cagtgacata
     6001 tttgcttaat ttcaatgcca ttttttctcc tcaatccata ccatccaaag tattcattaa
     6061 atcttccatg ataggaggtt gttttcattc gtattttgtg tcttcaaaag aaaatcattt
     6121 tagattgtaa gtactctgaa gagctttatc caataaggct tcttcttctg tgcttaaatc
     6181 tcaaatcaga agttctatta gtcatattag gttcattatt tgacttctaa gaagatctca
     6241 tggtcaatat tctacatctt ctatatttgg tggtatatca aaaggattca agtgttgttg
     6301 agaattggtc gctatattta cataggtacc accaactcta tcacaaagtc acttatattc
     6361 attttctgga tctattacaa ttgtgtcaat tcagtttatt aaatatctca atatttctaa
     6421 ttttactgta aatgattttc atgctccaga tgttgctaaa atacaactat tcatgtttgg
     6481 taacttccca ttaaatctat caaatattac taaggagcct gtataactgt taattcaata
     6541 taaaattccg gttttatcta taaggtcatt tgaaataaaa ggaaaacttc atgctagaga
     6601 tgatgtaaca gcacttcttg taattcacaa ctcatcatta cataagggaa gtatgctatt
     6661 aaatcattca tccattcttt gaatagagtg ttttacttta actcaatatc cagaaatttt
     6721 ttgttcaaac tttcttcaag tttcttttag tttatcttct ttttcttcgt aaatatttgt
     6781 ataatatcat aattcaaaat acctttcttc gcgagtagtt aatttttgtc taatttcttc
     6841 cacatctcta tattgttgtt caatttcagt atcaagcgtt atgccttttc ttatagattc
     6901 acttatttca gcttttaatt gggtagcttt ttgttttaac atagattgta tcgcagcatc
     6961 atcttttgga tataaaataa agctcatatc tcgttttgtg tcaaatgaaa atatttctct
     7021 tgttcgtaat gcatcgatat aagaaggata gctttggaca taataagatc tacccaaaag
     7081 tccagcaaca ttgaaatatg tgctatttgt ttcccaaaaa gatggtgcca tatgagattt
     7141 gtagtcacta attgttttat aatattgttt ttcagcagca gaaatgtatt gtcttaattg
     7201 gttggttaaa attttatcag aatactcttc atcaggcatt tctataattt gtcttacctg
     7261 ttcttttaaa gatagatttt taagtattgg gttttcttct tcgccatttt tttgattgga
     7321 agcacctttg gtctgtggta taatagattc aatcaatcta tctaggaatc acttttttgc
     7381 cactttttta ttctcttttt tgtcttcttg agttttgtta tcgttattac taataggaaa
     7441 atctataact catcattcct ttacttgtct agtgtggttt gtatcaaaga caatgttttt
     7501 taatttttgt ttcatgtttt tatttttttt aataaaataa taactcataa tcaattatat
     7561 tgaaaatctt tattttttac aaaaaaaaaa tgattttagg ctaacttggt ttgacttgtt
     7621 tgaaataagt tatctggtag cggtgagttc gtgaaagttt gtatgcaccc gggtggtata
     7681 caaactaaac tttataaact aaattatgtt ttctgtattt cgatctactt tgaaatgttt
     7741 ttggttaatt cttaatttag tataaacgat atccataaaa ttccttaaac tttctcattt
     7801 caaaataata ttgtatctgt atttattgaa cattttataa attagtgggg gagtagtata
     7861 gatttccaga ttatccattt ggtatttatc ctttaggtac aataaatctt tgtacattat
     7921 atctatttta ttaaacagtt tttcttctat ttcatctttg taagagatta cacaaacatc
     7981 agaaaatggt ggatatagat ttttttttct aaattgatta tcctctattg taaatagttc
     8041 tttatccatt ttacaagcgt ttctaatact ataaacttca ggattaaaag tttgtactat
     8101 ataattttta caagtatgac tagtaaaagt atcatataaa aaatgaaaat tattttttgc
     8161 agcagagtag tctgggatat ttagacctat atcagcattc aaaaatatga tcaaatccaa
     8221 ttcaactcac ttaatagggg tgtttagcaa tgaagttcaa atataaattt gaatatcttt
     8281 ttgttctaaa atttctttaa tttttttttg tgatctagta gtgtctgcat caattattac
     8341 tgtttttact ttgtattctg tttgaatata ttctgctatt ctttgagttc ctattcaaaa
     8401 ttccttaatt tccgcagaat gacaatgagg acattccaat gggaaattgt attgtgtttt
     8461 acatatatta caaagtccta ttttattttc attgatttta tagtagctaa tatttacgtc
     8521 acatctatta cactttggga catacccaca tttttgacag aaaataccgc ttgaaaatca
     8581 ttttttattt acgataattc atattttttt attatttttg atttgttttt ccaaagatgc
     8641 ttttgtgatt tctgaaaaaa tataatcggt ttctctcctc atatctacta aaaagattca
     8701 tttattttta tgtttgaata attcgtaatt cactttgtgg atatttttaa tataaaatta
     8761 tctgtattat tatgtatagg tataataatt tttttttcaa caaaaaaaga aaggaaggta
     8821 aaggcaaacc tttacctccc tcagaagcgt attgtttgga ctttcgtcct agaatttatc
     8881 gtattctagc ccgctcctaa attggcactt atattatata taaaaattct tgatttgtca
     8941 aatttttgat gacttttttt ctgaaagttt gtaagggtgg tagacagctt aatttgataa
     9001 gcttgtagct ttgaaaaatt gttcaatatt tttgatagtt tgattgaagt cttttgtttg
     9061 attacaaatt tcttttaatt cttttgatcc tggaatgcct tttaagtatt ggtgtaaata
     9121 tgttctaaag agtagaggag tatagtagat tttttcataa ttactttgga tatcaataga
     9181 ttcaatctct tgtaatttgg gttttatttt gttttttcat agattcttgg gatttaggtc
     9241 tgatgtaaat atttcaaatt tagctagtaa atttatgtgc tccagaattg tttcaaattt
     9301 ttcttgtatt gtcggactat gatttgtgaa tattcgtgga tttcatatag ctgattgacc
     9361 tatcattaca ccatctagat tctggatttt atttgtgatt tcattgaagt tttttattgc
     9421 tcagtttcat atgattttac agtttttatt agcttttttt ttcaattcat aaataaattc
     9481 tcggtctcaa tctccagaat aaagttcttt taatgttctt gcatgaattg tgatcatatg
     9541 acaaaaatta ctagcttctg tgagaaaatc catttgtttt tttttatctt cctcatttag
     9601 tcctgttcta cttttgatgc taaagggaac ttttagattt tttgaaagag ttttaattat
     9661 attcaaagta gattctttat tttttaataa agcagatcat catcaggatt tcataacatt
     9721 atttgctgga caacca
//
LOCUS       DAOSID010000018         9461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_6961_length_9461_cov_11.813098, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000018 DAOSID010000000
VERSION     DAOSID010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 9461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9461
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6961_length_9461_cov_11.813098"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1636)
                     /gene="argS"
                     /locus_tag="PK674_01615"
     CDS             complement(<1..1636)
                     /gene="argS"
                     /locus_tag="PK674_01615"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="arginine--tRNA ligase"
                     /protein_id="HOG15262.1"
                     /translation="MLNFYKKEFAEKIQKTSISLSFEEVLNNIEIVPSNIQGDLGFPC
                     FKLSKELKKAPNQIAQDFVTEIDDKNIIAVGPYINYLIPTKNFAEKLILEVKNQGNDF
                     GKLEKNNQEIVLEGWQPNTHKAFHVGHLRNALISESLSSCLRWAGYTVHPVAYPGDIG
                     AHVAKWIWYYINFYQGDMPTENFGEWAGKLYSQATNKLEENLDKYKPEVEDLQKKLED
                     GDIELNKIWKATREKCLLDFQDIFNELGCDIQKRYYESDVEKLGIKKVQELLDSKIAK
                     IGEGGAVIMDLEEYNLGIFLLLKSTGASLYSTKDIGLAFLKKEDFPEYVKSLCIVGSE
                     QEHHFNQLFKTLELMGFPHDQLKHISHGLVDLKDEKMSSRAGNVILYTELRDKLLLEA
                     QKVLGNRDFDQEKKDEIARQVAFAAMKFDILLPDASKKILFDPSQALSFEGETGPYIQ
                     YTHARASSLLKKGNLDLSQFDGSDLSEESEKGILIHLGQFSDYIFKSAQEYKPNYVAR
                     YLLDLSKLFNSYYNNTNKKITDSNSALALVFAVKQVL"
     assembly_gap    1659..1758
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1804..3087
                     /gene="tyrS"
                     /locus_tag="PK674_01620"
     CDS             1804..3087
                     /gene="tyrS"
                     /locus_tag="PK674_01620"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOG15263.1"
                     /translation="MDLRQERENRGLIYQFSDEKLFDLYNKGGQSFYIGFDPSADSLQ
                     IGNMFSVMAAVHLMKYGNKCYFLVGGATGMIGDPSGRSEERNFLSEEQLRHNQNCIYN
                     QLTSFLDNIKQNYNIDFNYEMVNNYDFFKGMDYLQFLREVGKYVTINYMINKESVKKR
                     IEDPDQSISYAEMSYMLIQGYDFFSLFSKYGVKLQLGGSDQRGNVTTGMDLISKKLGE
                     TSYCLTIPLITDATGAKYGKSAGNAVWVDKNKNSPYFVYQFFLNSDDSLVEKLLKVFS
                     LKSLKEIDEIVKKHEQDPASRYGQKELASWVVEVLFGKKAVQEVEKITQILFGSEDKI
                     NLIKGLDQEEILALKDATGGTKLEGNERKILDLCTQSGLTESNGEAKKMIQAGAIFCN
                     EEKVDDIQKILTKSDSINGIFLLRKGKKVFKVAQI"
     gene            complement(3175..6435)
                     /locus_tag="PK674_01625"
     CDS             complement(3175..6435)
                     /locus_tag="PK674_01625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOG15264.1"
                     /translation="MRNYIIPPKKTNFNSSNRYHSVYGYRDNRYRGQGFWKALFKVVL
                     ILGIIGFIVLFVLFQIRIKGRLPDITQVKNMVFSEATVITDRNDKVLYKLFDENREYV
                     DYSGINKNMINAIIAIEDQRYWEHKGLDTIGIFRAIVSKILNPKSKTQGASTIPQQLI
                     RNLLLTKDRKVERKLKEIVLTKKLDNVIEDMVKDNIGNLPSAEMNDKKKEFILELYLN
                     KIEFGNNAFGIEAAAQTYFNKSAKDLNILESSILASIPKGPTAYNPYINRVATIGVLK
                     ITDNLGNEYSLSDTGLKAEIFNKIESILSKADFSNKKDYSSFSKYYNGLIDFSIYYND
                     TKYNVKYSLGRKDLVLSRMFQDEYIEEEELKQAILQGMTLELKNGGFPIKAPHFVMWI
                     KKLLEEQYDNETLMNGGLVVKTTLDLDIQEIAEKSLLNNKKSLDVYGATNEAMVYLDA
                     EKGDILAYVGSFDYFNEDIGGQNDMVRVARQVGSAMKPMIYALGLSTLPIAIDTPIYD
                     IPFQIGPDRPRNSDGKFMGILPLKKALGYSRNIPAAKMITALGGQDVAVPFLRKMGLN
                     SLSVTGDYGYPLAFGAGEVPMLELASAYAHLAVSGPGEINPILEIRTNNGSLLYEKEE
                     KFQEAVVAPGVVYLMWNILSDTNNMPPERVGKYTVRGLQLGLKSGTSNMKTPKGDRAR
                     DGRLATYTPTRVAVFRGGNADGSPMYHNAYGGFLNAEAMREFWSTLLVNNYISDQGMS
                     ATEIAEVAISKISGKLATDSTPAEFVVRSTAYINSQPNQADEGAMPLEFDSSCGGLSS
                     PYTPASELVNGYIITPTTFMPNGMDLAEITSWWQWSTNPSLMWESSPEYSGKVIFNYD
                     NILLAMPQDYCENRSPQISEDIQLSIKNLSDGQKISTKPMIWFNVTAKNNIKRVSVSI
                     NDRVIGSKEYNGKSNDITDIISSNLGEETGSGELTLLAIDTQGYSNRTSIKLSIVSAD
                     TVPPFIMKDKTYVVKDGDRYRVVIILNDELSSVVGGTIKQGDKLLKKFDQNIGEFMTS
                     EPGVVSVVAKDSFGNELTDTLDIRDYIPGYKKEETNVSSDVSSGVESSSGSVLTGA"
     gene            complement(6854..8893)
                     /locus_tag="PK674_01630"
     CDS             complement(6854..8893)
                     /locus_tag="PK674_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG15265.1"
                     /translation="MKTFKKLFAYSVIFVLFAIVVPIFANAISYDTELTEAYAYAKSK
                     GITTMSNINSANMYGSLTRIAMAKMVANYVLDLKLQERDTSKQCTFSDVPASLDLAYG
                     NGVTKACQLGLMGVGITDFNPNGIVTRAEFGTVLSRALWGDENNGGEPYYKKHLQALK
                     DNRIMIQTNDPGRREVRGYVMLMMMRADEKYSSVVATGCTAEELLTCLLSTGDYQKCI
                     ANCSGDTTPVLSGKVVISRLDSAGLQTIPGNAQNVNVGTIKITAENNKVKVNSLTFKA
                     NNITGDIDSLDNIYVANDRILSSVQSFNPSTKEARLTFSPALILSAGKSETLNVYVNM
                     APRDDTPPDSRYSFSVSNLVADSPKSGIPLDLGTIKTSSDLVSEVEYSVVAGSTSNQI
                     AGKTNQSIVKVQLKLGNTGAIIKKVILTKSTAENQNFTGEAFYDIFANIKAYHSGNRV
                     ATVGIAENDLIISNLNISGDANEIIELELKGDIIYAGKKTSGAFVVDVNGTNIIDGPS
                     RYGAVVKAGYEYVSNPFDVDGLDLEIKNAMTGNLTVSPGDNDVVLYKATITSAVDLDV
                     LEYILDAGVNIDTYGGFKLNRIELYVNDSSEYINIAEFTGDTTPKFYQGNDKFFLYAD
                     QPVTIKVLGSFASTAKPQDFTFTFGITKVKDLLNNGTIVGSPTIKRATGKTISVK"
BASE COUNT         3189 a         1756 c         1231 g         3185 t
ORIGIN      
        1 ttaaaacttg ttttactgca aacactaaag ccaatgcaga gtttgaatca gtaatttttt
       61 tattagtatt attgtagtaa gaattaaaca gtttcgacaa atctagtaaa tatctagcaa
      121 cataatttgg tttatattct tgtgcagatt taaaaatata gtcactaaac tgtcctaagt
      181 gaatcaatat acctttttca ctttcttcac ttaaatccga tcaatcaaat tgagacaaat
      241 ctaaatttca tttcttaagc agactagaag cccttgcatg agtatattgt atataaggtc
      301 cagtttctca ctcaaaagac aaagcttgac taggatcaaa caagattttt ttagaagcat
      361 caggcaataa tatatcaaac ttcatcgcag caaaagccac ctgcctagct atttcatctt
      421 ttttttcttg atcaaaatct ctattcccca aaactttttg agcttctagt agtaatttgt
      481 cacgaagttc tgtatacaaa attacattcc cagcacgaga tgacattttt tcatctttta
      541 gatcaactaa tccatgagaa atatgtttta attgatcatg agggaatcac atcaactcca
      601 aagtcttaaa taattgatta aaatggtgtt cctgctcaga acccacgata caaagcgatt
      661 taacatattc agggaaatct tcctttttca aaaaagctaa cccaatatct ttggtggaat
      721 acagagatgc tccagtagac ttcaaaagca aaaatattca taagttatat tcttccaaat
      781 ccattatcac cgctcctcac tcccctattt ttgctatttt ggaatctaat aattcttgaa
      841 cttttttaat tcaaagcttt tccacatcac tttcgtaata tcgtttttgg atatcacatc
      901 aaagctcatt aaaaatatct tgaaaatcca ataaacattt ttcacgagtt gctttccaga
      961 ttttatttaa ttcaatatct caatcttcaa gttttttctg caaatcctcc acttcaggct
     1021 tatatttatc taaattttcc tcaagcttat ttgtagcttg tgaatacaat ttccccgccc
     1081 attctcaaaa gttttctgta ggcatatctc actgataaaa attgatataa taccaaatcc
     1141 atttagctac atgcgctcaa atatccccgg gataagctac tgggtggaca gtatatcccg
     1201 cccatctcaa gcaagacgaa agagattctg aaatcagagc atttcttaag tgaccaacat
     1261 gaaacgcttt atgagtattt ggttgccatc attccaaaac aatctcttga ttatttttct
     1321 ctaatttccc aaaatcatta ccttgatttt taacctctaa aatcaacttt tcagcgaaat
     1381 tttttgttgg gataagataa tttatataag gtcagacagc aataatattc ttatcatcaa
     1441 tctctgtaac aaaatcttgt gctatttgat ttggtgcttt ttttaattct ttggaaagtt
     1501 tgaaacaagg aaatcctaaa tctcattgga tgttggatgg aacaatttca atattgttca
     1561 aaacctcttc aaaagacaaa cttattgatg ttttttgaat tttttctgca aattcttttt
     1621 tataaaaatt aagcattatt ttttcattaa aaagtaagnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnga aaatattata caataactac acatttaatt tattctttaa
     1801 aaaatggatt taagacaaga acgagaaaac cgtggactca tctaccaatt tagtgatgaa
     1861 aaactttttg atctatacaa caaatgaggt caaagttttt atatttgatt tgatcctagt
     1921 gctgatagtc tacaaatagg aaacatgttt tccgtgatgg cagcagtaca tttaatgaaa
     1981 tacgggaaca aatgttattt cttagtatga tgagcaactg gtatgatctg agatcctagc
     2041 ggtagaagtg aagaaagaaa ttttttatct gaagaacaat taagacataa tcaaaattgt
     2101 atctataatc aattaacaag ctttttggac aatataaaac agaactacaa tatagatttc
     2161 aactacgaaa tggtaaataa ttatgacttt tttaaatgaa tggattattt acaatttctt
     2221 agagaggttg gaaaatatgt cactataaat tatatgatca acaaagaatc tgtaaaaaaa
     2281 agaatcgaag atcctgatca atctatctca tatgctgaaa tgagctatat gctaatccaa
     2341 ggatacgact tttttagtct atttagcaaa tatggagtaa aactacaatt atgatgatca
     2401 gaccaacgat gaaatgtaac tacatgaatg gatttgatat ccaaaaaact atgagaaacc
     2461 tcttattgtc ttacaatacc tttaattacc gatgctacat gagccaaata tggaaaatca
     2521 gctgggaatg ctgtttgggt agataaaaac aaaaatagtc cttatttcgt ttatcaattc
     2581 tttttgaact cagatgattc tttagttgaa aaattattga aagtattttc actaaaatct
     2641 ctaaaagaaa tagatgaaat tgtcaaaaaa catgaacaag atccagcatc tagatacgga
     2701 caaaaagaat tagcatcttg ggtggtagaa gttctattcg ggaaaaaagc tgtacaagaa
     2761 gtagaaaaaa tcactcaaat attatttgga agtgaagaca aaataaattt aattaaatga
     2821 ttagatcaag aagaaatttt ggctctaaaa gatgctactg gtggcaccaa attagaaggg
     2881 aacgaacgaa aaatattaga tctttgtact cagtcatgac ttactgaatc aaactgagaa
     2941 gctaaaaaaa tgattcaagc atgagcgata ttttgcaatg aagaaaaagt agatgatatc
     3001 caaaaaattc taacaaaatc tgatagtata aactgaattt tcttattaag aaaaggaaaa
     3061 aaagtattca aagtcgcaca aatatagttt tattgttttt ttacaacaca ccacccgggt
     3121 gctgctcgca aagctaatca attgcacccg ggtggtgtgt gtcgcaagta acttctaagc
     3181 ccctgttaaa actgatccgg aactagattc aactcatgaa gaaacatctg aagaaacatt
     3241 agtttcttct tttttatatc aaggaatata atctcttata tctaaggtat cggttaattc
     3301 attaccaaaa ctatctttgg ctactactga aactactcat ggctctgagg tcataaattc
     3361 acctatattt tgatcaaact tttttaacaa cttatctcat tgttttattg ttcaaccaac
     3421 aacactagaa agttcatcat ttaatataat taccactcta tacctatccc catcttttac
     3481 tacataagtt ttatctttca taataaatgg tggcactgta tctgcagaaa caatggacaa
     3541 cttaatcgaa gttctatttg aatatcactg agtatcaatt gccaataaag taagttctca
     3601 acttcaagtt tcctctcaaa gattagacga aataatatca gtaatatcat tagatttacc
     3661 gttatattct ttagagccaa ttactctatc attgatacta acagaaactc ttttaatgtt
     3721 atttttagct gttacattaa accaaatcat aggttttgta gatatttttt gtccatcact
     3781 taaattcttg atactcaatt gaatatcttc actaatttgt gggcttctat tttcacaata
     3841 atcttgtggc atagcaagca agatgttgtc gtaattaaat attactttac ctgaatactc
     3901 tggactagat tcccacatca aggaagggtt tgtagaccat tgccaccaag aagtaatttc
     3961 tgctaaatcc attccatttg gcataaatgt agtaggagta ataatatatc aatttaccaa
     4021 ttcactagct ggtgtatatg gactagataa tcatccacaa gaagagtcaa actccaatgg
     4081 catagctcac tcatcagctt gatttggttg agaattgata taagccgtag atcttacaac
     4141 aaattcagct ggcgtagaat ctgtagccaa ttttcaggaa attttggaaa tagctacttc
     4201 tgcaatttct gtagctgaca ttcattgatc tgaaatataa ttgttcacta ataaagtaga
     4261 ccaaaattct ctcatagctt ccgcattcaa aaatcatcca taagcattgt gatacattgg
     4321 cgaaccgtct gcatttcatc atcgaaatac agccactctt gtaggagtgt aagtagctaa
     4381 tcgtccatct ctagcacgat ctcatttggg agttttcata tttgaagtac cagattttaa
     4441 tcatagttga agaccacgaa ctgtgtattt tcctactcgt tctggtggca tattattggt
     4501 atcagataat atattccaca tcaaatatac aactcatgga gcaaccacag cttcctgaaa
     4561 cttttcctct ttttcataca ataaagatcc attatttgtc ctaatctcca aaatcggatt
     4621 tatttctcac ggcccagaaa cagctaaatg agcatacgca ctagctaatt ccaacatagg
     4681 aacttcccct gctccaaaag ctaaaggata tccataatct caagtaacac tcaacgaatt
     4741 caatcacatt tttctaagaa atggaacagc tacatcttgt caccccaaag ctgttatcat
     4801 tttagctgct ggaatatttc tactataacc aagtgctttt tttaaaggta aaatacccat
     4861 aaattttcaa tctgaatttc taggtctatc tggtcatatt tggaaaggaa tatcgtaaat
     4921 cggagtatct atagctatag gtaaagtact tagacccaaa gcataaatca taggtttcat
     4981 agccgatcca acctgtctag caactctaac catatcattt tgtcatccaa tatcttcatt
     5041 aaaataatca aaagatcaaa catatgccaa aatatctcat ttttctgcat ctaaatatac
     5101 catagcttca tttgttgctc catacacatc caaagatttt ttattattca acaatgattt
     5161 ttctgctatt tcctgaatat ctaaatctaa tgttgttttt acaactaatc atccattcat
     5221 caatgtttcg ttatcatatt gttcttccaa tagttttttg atccacatca caaaatgtgg
     5281 tgctttgata gggaatcatc aattttttaa ttccaatgtc attcattgta atatggcttg
     5341 ttttaactcc tcttcctcta tatattcatc ttgaaacata cgagataaaa ctaaatcttt
     5401 tcttcataaa gaatatttta cattatattt tgtatcgtta taatatattg aaaaatcaat
     5461 caatcaattg taatatttac taaaagatga ataatctttt ttgtttgaaa aatcagcttt
     5521 tgataatatt gattctattt tgttaaatat ttctgctttt aatcatgtat cactcaaaga
     5581 atactcgttt cataagttat ccgtaatttt taaaactcat attgtggcta ctctatttat
     5641 ataaggattg tacgctgtag gtccttttgg tatagaagcc aatatagaag attccaaaat
     5701 attaagatcc ttggctgact tgttgaaata agtttgagcc gccgcttcaa ttccaaaagc
     5761 attatttcca aactctatct tgtttaaata taattccaaa ataaattcct tttttttatc
     5821 attcatctca gcagacggca aatttccaat attatctttt accatatcct ctattacatt
     5881 atccaatttt tttgtaagaa caatttcctt taatttcctt tccacttttc tatccttagt
     5941 caaaagaaga tttctaatca attgttgagg aatggtggaa gctcattgtg ttttgctttt
     6001 gggatttaat attttactaa ctattgccct aaatattcat atagtatcaa gtcatttatg
     6061 ctcccaataa cgctgatctt caatagcgat aatagcattt atcatatttt tatttatacc
     6121 agaataatct acatactctc tattttcatc aaaaagcttg taaagaactt tatcgtttct
     6181 atcggtaata accgtagctt cagaaaaaac catattcttt acttgtgtaa tatctggtaa
     6241 tcttcatttt atccttattt gaaaaagtac aaacaaaaca ataaatcaaa taattcacaa
     6301 gataaggact actttaaata aagctttcca aaatcactgt catctatatc tattgtccct
     6361 atatcaatat actgaatgat acctgtttga actattaaaa tttgtttttt tgggtggaat
     6421 tatataattt ctcatttaac agaattatta ttctatataa aaactgtagg atatactagt
     6481 aataaaagct cttttcataa acacactagt tatcagcaag tagtttataa aaattatcaa
     6541 tcaaaaaaca aatgaagata cgaaaaaagt catcaaccaa aagaaacttc cattcatatt
     6601 cgtaatattg ctggtatcaa aacacatttt ttccccttta aatcattcca aacacctctt
     6661 tcccaagcac atcttgctca aggtagaata aagaggtccc gctgaacttt ggaaaatatg
     6721 tctggaaaga attgtttgtt cgagatttac cgagtaattg tgataactga ttgttctcat
     6781 ccggaaaaaa ttgtttgttc aagattcacc aaggaaaaaa aagaggatat cagccattta
     6841 aattcggcta ctattatttt accgatatag tttttcaggt agctctttta atcgttggac
     6901 ttccaacaat tgtcccatta tttagcaaat ctttaacttt agtaatacca aatgtgaatg
     6961 tgaaatcttg tggtttagcg gtagatgcaa aacttcccaa cactttgata gtaacaggct
     7021 ggtctgcata aagaaagaat ttatcattcc cttgatagaa tttaggagtt gtatctcccg
     7081 taaactcagc gatattaata tattccgaag aatcatttac atacaattca attctattca
     7141 gtttaaaacc accgtaagta tcaatattaa ctccagcatc tagaatatat tccagcacat
     7201 caagatctac agcggaagta atagtggctt tgtacaacac cacatcatta tcgccaggag
     7261 aaactgtcag atttccagtc atagcatttt ttatctcaag atcgagtcca tcaacatcaa
     7321 aaggattgga aacatactca tatccagctt tgactacagc accatatcta ctaggaccat
     7381 caattatgtt cgttccgttt acatccacaa caaatgcacc tgatgttttt ttcccagcat
     7441 agataatatc tcatttcagt tcaagttcta taatttcatt agcatctcca cttatattaa
     7501 gattggaaat aataaggtca ttttctgcaa tacctaccgt agcaaccctg ttaccactgt
     7561 gataggcttt tatattagca aaaatatcat aaaaagcttc tcaagtaaaa ttctgatttt
     7621 ctgctgtact ttttgtaaga attactttct tgattatagc accagtatta ccaagtttca
     7681 actgaacttt gacgattgat tggtttgttt ttcaagcaat ctgattggaa gtacttccag
     7741 caactacact atattctacc tcacttacaa gatcagaact ggttttgata gtacctaaat
     7801 ccagtgggat cccagatttt ggactatcag caacaagatt acttacacta aaactatatc
     7861 ttgaatcagg aggagtatca tctcttgggg ccatatttac atagacattc aatgtttccg
     7921 atttcccagc agataagatt aatgcaggag aaaatgtaag cctagcttct tttgtggatg
     7981 gattaaaact ctgaacagaa gatagtattc tatcattagc tacataaata ttatcaagac
     8041 tatcaatatc tcctgtaata ttatttgctt tgaatgtaag agaatttact ttaactttat
     8101 tgttttcagc agtaattttg atagttccta catttacatt ttgtgcattc ccaggaatag
     8161 tttgaagtcc agcagaatca agacgactta ttacaacttt accagaaaga acaggagtcg
     8221 tatcaccact acaattagct atacattttt ggtaatcccc agtagacaaa agacaagtaa
     8281 gcaattcttc agcagtacag cccgtagcaa caacagaact atatttttca tcagccctca
     8341 tcatcataag cattacatat cccctcactt cccttctccc aggatcattg gtttgtatca
     8401 taattctgtt atctttcaaa gcttgaagat gttttttgta atacggttct ccaccattat
     8461 tctcgtcccc ccacaaagct ctggacaaaa ccgttccaaa ttcagctctg gttacgattc
     8521 cgtttgggtt gaagtccgta atcccgactc ccataagccc tagttgacat gcttttgtca
     8581 caccattacc gtatgcaaga tccaaactag caggcacatc cgagaaagta cattgtttac
     8641 ttgtatctct ttcttgtaat tttaaatcaa gaacatagtt ggctaccatt ttggccatcg
     8701 ctattcttgt caaactaccg tacatgtttg cactattaat attactcatg gtggtaattc
     8761 atttgctttt tgcatacgca tatgcttcgg tcaattccgt atcgtatgaa atagcattag
     8821 caaaaatagg taccactatt gcaaacaaaa caaaaattac tgaataagca aaaagttttt
     8881 taaatgtttt catctgtaca ttaaaaaaaa taaaaaacaa taaatctaat taccaaattg
     8941 cgtgtcagtg atttcatatc atttccaatc catatcacac tccttcttcc aattctaaac
     9001 taaaagaaag tggtagattt agtaggattt tgtcccaaca tcaccaacaa aattagacat
     9061 atttcatcat cttcattact gaatttatct atatttcaaa ctttttcaag taattttgaa
     9121 atccagttca acaattaaca atgagcaatc cctaatactt cgttctaaat cagtccaaaa
     9181 ccactctact gtcctaaatc accctaaacc cacttgcttc tcttaaatca ccccccaacc
     9241 cccctacttt taattctaaa tcaccccaaa cccctctact tttaattcta aatcacccca
     9301 aacccctctt atattttaaa tcatccccaa acccacttgc ttctcttaaa tcaccccaaa
     9361 ccacttactt ctcttaaatc atcccccaac cccctacttt taattctaaa tcaccccctt
     9421 aatccccctc tactatagga aatatattga aaaatatata c
//
LOCUS       DAOSID010000019         9425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_6985_length_9425_cov_11.641622, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000019 DAOSID010000000
VERSION     DAOSID010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9425
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6985_length_9425_cov_11.641622"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..314)
                     /locus_tag="PK674_01635"
     CDS             complement(93..314)
                     /locus_tag="PK674_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOG15266.1"
                     /translation="MKKKYQIVIERDENNYFVGEVVGLLACYTQAKTVPELLSRLLEV
                     TEGSIDLVRDIQGKDSLQKFNLSMKIEYA"
     gene            complement(457..1023)
                     /locus_tag="PK674_01640"
     CDS             complement(457..1023)
                     /locus_tag="PK674_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15267.1"
                     /translation="MGLGMDEARIDSGLDNSEYSKEFGYLNQLRQSLDFSIFNKPGLC
                     KSNFFSTIAWYIELLKKENSNNREEKMQELIDKTKTQNFVEMFRDLDKFLSEKQIEWH
                     EAMGSINAKLLRIGQNRIDDMYKILDDEKFPAVFPGILPDAIPERYKLWLQENGIEEN
                     VDEYNRRTKVKDISNVLNQQKISDIPNQ"
     gene            complement(1451..1831)
                     /locus_tag="PK674_01645"
     CDS             complement(1451..1831)
                     /locus_tag="PK674_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15268.1"
                     /translation="MNTPKDNINTSRTQEQLREEIKSLTKDNDLYCVAEIPVFFNKKT
                     NQAYSYINGKRQTMGDKEFQDRASELDLNKHGDKQIINTIFTQDIVETFKEYLPKAYE
                     YHVFFLKRNEKKNKDQIKEILNML"
     gene            complement(1870..9081)
                     /locus_tag="PK674_01650"
     CDS             complement(1870..9081)
                     /locus_tag="PK674_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15269.1"
                     /translation="MVNISLKEIAKFDDNAQDISLKDLETSFDNLIKKEKDINKFVSK
                     TLVPKLEKNLEQYNFNSENIQTEILEKLKNEKFSDISKNINTIKVYQLCQALQNKNAN
                     ITIDGILGESMVNDLVNNRLQFYQEGGLGLLDGESISYERNKLLINTWFGDVEVSYDS
                     NKDTLFEILETKENLVKFINKEKEDLFDDYINKNIDNKSSIVEVGINFFAEDGLIIVN
                     FLDKTISYHISDFKNPNEILLHLSGQKIEVEKCEQERNHINSILDKDKLTDEEIKLVN
                     DWVKQIPKEYKSMLKETQDKMEAKIAKISSEVNKLIARNEDGNVNYYSKEDIKLIQLG
                     ANVCFGENITIDGNRGSLFYSSENNKNQKLTNFYIYRQELITRQGVIDARKDFKMQEF
                     NDLLNIPKLQELLETLKDVDKQNKVFSKFLDFFLEIRVSGTYFENQIGSETILPVLNY
                     MINAEKCKNVSVEEYFGLIEQNHEETIKLKKLVKDGGYKTMYEYYEAKAKEFETISID
                     QLKDPKTIEEFKRAGYKNVGEFYKDIQSAFSQEPQKSMDVDDYFKEQEDMFNNMLEMQ
                     NSIKYSTMGEYFENSSVSLMSTIVDANKINKITDQIEHLEQRLEKMEPVKGIYQETEE
                     ASGLSYLDSRGVSREVGNIISSERGSSLWSEWGQTRVKLLEQKLKIYEITRDEDYLLW
                     DIYLDMKNFFSEKDIKMIFELKKSKKNNELSNKGFTGDIEESSEEVLSKNSLTSIITD
                     DLDKFPKEFGWLGKSISEYGVSYADYTTKINNAEKIIEEDIKKRDSTNDIISQFESED
                     EFYLTRDVKREEYAKLFPHDAEANKNSRKGTIHDYTGTKSVYHRNFNDRHKAVPGELY
                     KLHVPGGGYIIVRITSEAHYAVNSLISKKQVGQKLIIPKHNYEYGKMNSITFNYYKVD
                     RSESKKNLLTVKDFCIAHKNKNGDIVLSDSKGNVVEIISDADQKSAKNTIQKAYAYMN
                     TYEMKEIDSSFDLFEKDFREMNKLFQKFQKATKLDEDGNVKKNANGEIDAYKGISETD
                     VEELRNLSTQFIKTKLDKYEASVGKRKKSLELLKSLQASGSLDNQLDTHIRSQIKTLT
                     DMINFFGSGNARKIFEMMASARSNEKWRQVARIWIQDNLIQFIFAIAGAVAAIVRAAP
                     SGGASVWAYIKGMTLYTAAVGALGGMVGARIGQLTNEGIQNIFFAKTVNGYTIKYDNP
                     TDVELLFRGEENGGISVRDFFVGLTTEFIMGTITTALFIKVGKIIGSYLTSAIKINAA
                     NLRPSALVGKQISKLKNGFNRIGGSINKLSPSNKVIANNSIKSFGKAFKLEFGEELFE
                     ESFENIMESIDPILGGIATVINCLSGPNPGKIFQQHNVAGGLTTIEGNKIVQGMTYNS
                     DNISELEEYFTKEQKYTKIKSDGRKIRFKKTMNQNDVMTRKDGTKVNEHVIELVYSNA
                     STFLNNSAEFFAKFGMTINHDSKNNEVIVHSKAQLEAFTLAVGLGGLGEVTINPDGSA
                     KFVNGKDVVNIKPSKSKTFALGGIETYFEGKVSTLGFSETQALQNSQKMDKVLSEITT
                     DNQTKVLKELKQNIEEQWNKLLEDGKVTGELDLSEAQLLSILDAHEQDGVLGQLNQPQ
                     LRRKVKTLSETITDPDVRRFLLEAGFSGKEVFDLNKIYKDLTTPYMSIYEGLKFRGIN
                     LDTHLMEAFLDKIKKSENIPQIIEAYSDFSEGCVKEILEISESSFFSHDVQEEINNII
                     KIRFMPIIESAINLMSEGKGKQKMEHLLQQIQEFTKKLFTPIHELADRFIKENANIIG
                     NKEKTFHLILTKIDPTVGYYLPQYNLLNSINTAIKQYGVEVLEKYFIQYKDSSFSLEN
                     IQNILENPLKASEVWEKKKIEKQVPLESSGEMAFDYEGFPIYTKTETNEDCIIFNKNT
                     GMLNINTGGKKTKELILTGSIIGSESIPPNMRWKERVIHSDIDIDVLAQSIVRKGGFT
                     EAFGSSDKVAVDEITGFNGEPLYIVTYNGNHRTAAAKQAGLSHIIAEVTKKPEGKQEV
                     IAHTPGIVGIREERIQKGYINGKIIKGNGTTYLELESGVFDGCHLSSFELKKYASFYE
                     KYFGSIPEHTKNIIDDMLGLSNLLETETNLNRTKELENLNVIEASEKDKLNQRQPEVE
                     GESTLEKAEALLTYLSKSKETFENIFVYLTDKIGGERKGAKFIFKDPNRIVQKADREY
                     GGDVTKIFDAMRGVQVFDTVDQLNQAYIHLEIYCKENNIEVIKVKNRLSIPQIGDVLM
                     NLKFPDGSIVELKFYITEQYIAQDGETIIDQQTPVYVNGEVKEWNDNFFTQKEQELVS
                     KLGAKPHNFAEKILHLELDSKKSINNHTFYEIERCLPNGNMEQRSLEKVKAFDKFAQG
                     IGLSPSDVELFRQKLIAIQVVNGNLAKDAYINRVTGINK"
     gene            complement(9088..>9425)
                     /locus_tag="PK674_01655"
     CDS             complement(9088..>9425)
                     /locus_tag="PK674_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15270.1"
                     /translation="GGGGYNHIVRYFYYLISNMERKKEFLIKVLNKIKTYRPLAEGFL
                     SLLESEYATEEIMDKLILALKESLDEIKEENEKTKIEKSLELIQKIKEEEMEEKIADG
                     DLDDLLDNI"
BASE COUNT         2798 a         1566 c         1104 g         3957 t
ORIGIN      
        1 aaaaaaactt atgactttat tttgtcctaa tatggacaaa tcataagttg ataattattt
       61 tttcaagtaa tttcggagaa ataggactga ttttaggcat attcaatttt catagaaaga
      121 ttaaattttt gcaaactatc ttttcactga atatctctaa caagatcaat acttcattct
      181 gtcacttcaa gtaatctaga aagcaattct ggaactgtct ttgcctgtgt ataacaagca
      241 agcaatcaaa ccacctctca gacaaagtag ttattttcat ctctttctat tacaatttga
      301 tatttctttt tcatagaact tatttatctt atataaatat atatgcttat attatattta
      361 tccacgaata aaaagcaaat tatttttatt tggagtctgt attaagtcta atttatcttg
      421 tatttttatt gcttgaattt ttatacagaa ttatagttat tggtttggga tatctgaaat
      481 tttttgttgg ttaagtacat tacttatatc tttaactttt gttctcctgt tatactcatc
      541 aacattctcc tctattcaat tttcttggag ccataattta taccgttcag gtattgcatc
      601 aggtaaaatt catggaaata ctgctgggaa tttttcatca tctaatattt tgtacatatc
      661 atctattcta ttttgtcata ttctcaataa ttttgcattg attgatcaca tagcctcatg
      721 ccattctatc tgtttctctg ataaaaattt atctaaatct ctaaacattt ctacaaaatt
      781 ttgagttttt gttttatcta taagttcttg catcttttct tctcgattat tagaattttc
      841 ttttttcaac aattcaatat accaagctat agtagaaaaa aagttacttt tacataaacc
      901 aggtttatta aatatagaaa aatcaagaga ttgccttaat tgatttaagt atcaaaactc
      961 ttttgagtac tcagaattat ctaatcctga atcaatcctt gcctcatcca ttcctagacc
     1021 catttttata aatcataaaa gattaaaacg caatgatatt ataaggttat tgttttattt
     1081 gtcaatatta aaactgaata ttattatgct atatacttct gagtttataa actctattct
     1141 caacaaagaa ttggaattgt gtattgtccc ctatcttatt taagagcttt tagtaattga
     1201 taagtgcttt ctatgaagaa tccgatgtgc aattgacttg aaatatgctg aaagcttgga
     1261 acttgaagtt attttgtatg gttctctttc taagaaatta tgagttttca aaggggatgt
     1321 aaagagtttt tagttttatt tcttgaagtg agtaaacagt atgactatga gaaatgattc
     1381 atatgtattt tattcagcat gatgaaaatc tcttaaaggg gattgttttt gggctttttt
     1441 tattaaagta ttaaagcata tttaatattt ctttaatttg atccttgttt tttttttcat
     1501 ttctcttaag aaagaataca tgatattcat aagcttttgg aagatattct ttaaaagttt
     1561 ctacaatatc ttgtgtgaat atagtattga tgatttgctt gtccccatgt ttattaagat
     1621 caagctcact cgctctatct tggaactctt tatctcacat cgtctgtcgt ttaccattaa
     1681 tataactata tgcctggttc gttttcttgt taaaaaagac tggtatttct gctacacaat
     1741 ataaatcatt gtcttttgtt aaagatttta tttcttctcg taattgctct tgagttcgag
     1801 aagtgtttat attgtctttt ggtgtattca ttgttattat tagtaaagaa taaatgtata
     1861 aagattatat tatttattga ttcatgtaac tctatttata tacgcatctt ttgcaagatt
     1921 accgtttact acttgtattg caataagttt ttgtctaaat aattcaacat cactaggaga
     1981 taatcatatg ccttgtgcaa acttatcaaa tgcttttact ttttcaagag atcgttgctc
     2041 catatttcaa tttggcaagc aacgctctat ttcatagaag gtatgattat taatagattt
     2101 tttactatcc agctctaagt gcaatatctt ttctgcaaaa ttatgtggtt ttgctcataa
     2161 tttactcacc agttcttgtt ctttctgagt aaagaagtta tcattccact cctttacttc
     2221 tcaattaaca tatactggtg tttgctggtc tataatagtt tctcaatctt gagcaatata
     2281 ctgttctgta atataaaact ttaattcaac gatagatcag tctgggaatt ttagattcat
     2341 aagcacatct catatttgtg gtatactcaa gcggtttttt actttaatta cttctatgtt
     2401 gttttcttta caatatattt ctagatgtat ataagcttga tttaactgat ctacagtatc
     2461 gaatacttgt actcacctca ttgcatcaaa tattttggtt acatctcaac catattccct
     2521 atctgctttt tgtactatcc tatttggatc tttaaatata aactttgctc attttctttc
     2581 ccctcctatc ttatcagtta agtacacaaa tatattttca aatgtttcct tacttttgct
     2641 taaataagtt agtaaagctt ctgctttttc aagtgtgctt tctcattcta cttcaggctg
     2701 tctttgatta agtttatctt tttctgatgc ttctataacg tttaaatttt ctagttcttt
     2761 ggtgcggttt aggtttgttt ctgtttcaag aagatttgaa agaccaagca tatcatcaat
     2821 aatatttttt gtgtgttctg ggattgagcc aaagtatttt tcataaaatg atgcatattt
     2881 ttttaactca aagcttgata aatgacatcc atcaaaaact caagattcca actctaaata
     2941 agttgttcaa tttcctttga taatttttcc attaatatat cacttttgaa tacgctcttc
     3001 tcgtattcaa actattcaag gagtgtgagc tataacttct tgttttcatt caggtttttt
     3061 tgttacttct gcgataatat gggaaagtcc tgcttgttta gctgctgcag ttctgtgatt
     3121 cccattataa gtaacaatat aaagaggttc tcaattaaaa cctgtaattt catctacagc
     3181 aactttatca gaactaccaa atgcttctgt aaatcagcct tttctaacaa tagattgagc
     3241 gaggacatca atatcaatat cggaatgaat aactctttct ttccatctca tattgggagg
     3301 gatgctttct gaaccaatga ttgacccagt taatattaat tctttcgttt tttttcatca
     3361 ggtattaata ttcaacatac ctgtattttt attaaaaatt atacaatctt catttgtttc
     3421 tgtttttgta taaataggga atccttcata atcaaacgcc atctctcaag aagattctaa
     3481 gggaacttgt ttttctattt ttttcttttc ccaaacttca cttgctttaa gtgggttttc
     3541 taaaatattt tgaatatttt ctaatgagaa agagctatct ttatactgta taaaatattt
     3601 ttctaacacc tctactcaat attgctttat ggcagtattt atactgttta atagattata
     3661 ttgaggaagg taataaccta ctgttggatc aattttagta agaattaaat gaaaagtttt
     3721 ttctttattt cagataatat tagcattttc ttttataaat ctgtcagcaa gttcatgaat
     3781 tggagtgaaa agtttttttg taaattcttg aatttgttgt aaaaggtgtt ccattttttg
     3841 ttttcccttt cattctgaca taagatttat agcactttca ataattggca taaagcgaat
     3901 tttaattata ttatttattt cttcttgtac atcatgagaa aagaaagagg attctgaaat
     3961 ttccaatatt tcttttacac acccctcaga aaaatcagaa taggcttcaa taatttgagg
     4021 gatattctca ctttttttta ttttgtctag gaatgcctcc attaaatgag tatctagatt
     4081 tattcatctg aattttagtc cttcatatat gctcatatat ggagttgtca aatctttata
     4141 aattttattt aaatcaaaaa cttctttccc actaaatcca gcctccaaca aaaatcttct
     4201 cacatctgga tctgtaattg tttctgaaag tgtttttact tttcttctca attgtggctg
     4261 gtttaattgc cctaatactc catcttgttc atgagcatca aggatagaaa ggagctgtgc
     4321 ttctgaaaga tctaattctc aagttacttt tcaatcttcg agaagcttat tccattgctc
     4381 ctcaatattt tgttttaatt ctttgagcac tttggtttga ttgtctgtcg taatttcgga
     4441 aagaacctta tccatctttt ggctattttg taatgcctga gtttccgaga atcaaagtgt
     4501 cgaaactttt cattcgaagt atgtttctat tcctcataga gcgaaagttt tacttttact
     4561 tggcttgata tttactacat ctttgccatt aacaaatttc gctgatccat caggattaat
     4621 agtaacttca cccaatcaac ccaatcctac tgctaaagta aatgcttcta gttgagcttt
     4681 actgtgaaca atcacttcat tatttttaga gtcatgatta atagtcatac cgaacttagc
     4741 aaaaaattca gcactattat ttaggaatgt tgaggcattt gaataaacaa gttctataac
     4801 atgctcattt acttttgttc catcctttct tgtcatcaca tcattctgat tcatcgtttt
     4861 tttgaacctt atttttctac catcagactt tatttttgta tatttttgtt cttttgtaaa
     4921 atattcttct aactcactta tattgtcaga attatatgtc attccttgaa caattttatt
     4981 tccttctata gttgtcaatc atcatgctac attatgttgt tgaaatattt ttcctggatt
     5041 tggaccagaa agacaattta taactgttgc tatacctcat aaaattggat caatactttc
     5101 cattatattt tcaaatgatt cttcaaataa ttcctctcca aactctaatt taaaggcttt
     5161 accaaaactt ttaatagagt tatttgctat aaccttatta cttggagata atttattaat
     5221 agacccccca atcctattga atcaattttt taatttgctg atttgtttac ctactagtgc
     5281 actaggtcta agattagctg cattaatttt tattgcagaa gttaaatagc ttcctataat
     5341 ttttcacact tttataaata aggctgttgt aattgttcac ataataaatt cagtagtcaa
     5401 tccaacaaaa aaatctctaa cacttattca tcaattttct tctcctctaa aaagaagttc
     5461 tacatctgtt ggattatcat attttatagt atagccattc actgtttttg caaaaaatat
     5521 attttgtatt cactcatttg taagttgacc tattcttgct ccgaccatac ctcctaaggc
     5581 tcatactgcg gctgtatata aagtcattca tttaatatag gcccaaacag atgcccctca
     5641 agatggtgct gcccgtacaa tcgctgctac tgctcccgct atagcgaaaa taaattgtat
     5701 taagttatct tgaatccaaa ttcttgcaac ttgtcgccat ttttcattag atctagctga
     5761 tgccatcatc tcaaaaattt ttcttgcatt tccagatcca aaaaaattta tcatgtctgt
     5821 caaggttttt atttgtgatc ttatatgagt atcaagctga ttatctaatg atcatgaagc
     5881 ttgtagtgat ttcaataatt ctaatgattt ttttcgttta cctacagacg cttcatattt
     5941 atctaatttt gtttttataa attgagtaga caaattcctt aactcttcta catcggtttc
     6001 tgatatacct ttatatgcgt caatttctca attagcattt tttttaacat taccatcttc
     6061 atctaactta gtagcttttt gaaatttttg aaacagtttg ttcatttctc taaaatcttt
     6121 ctcaaataag tcaaagctac tatctatctc tttcatctca taagtattca tataagcata
     6181 tgctttctgg atagtgtttt ttgctgattt ttgatcagca tccgatatga tttctacaac
     6241 attaccctta gaatcagaca aaacgatatc gccattttta tttttatgag ctatacaaaa
     6301 atctttaact gtgagtaaat tttttttact ttcacttcga tctactttat aataattaaa
     6361 tgttatactg ttcatcttac catattcgta attgtgtttt ggtataatga gtttttgtca
     6421 gacttgtttt ttagaaataa gactattaac tgcgtagtgt gcctcgcttg ttattctaac
     6481 aataatatat catcctcatg gtacatgaag tttatataat tctcctggga cagccttatg
     6541 acgatcatta aaatttctat gataaacact cttcgttcat gtataatcat gtatagttca
     6601 tttacgagaa tttttgttag cctctgcatc gtgtgggaat aacttagcat actcctctcg
     6661 tttcacatct ctggtaagat aaaactcatc ttcagattca aattgtgata ttatatcgtt
     6721 ggtactatct cgttttttta tatcttcttc tattattttt tcagcattat ttatttttgt
     6781 tgtataatcg gcatatgata ccccatactc gctgatactt tttcctaacc atcaaaattc
     6841 tttaggaaat ttatcaagat catccgttat aatgcttgtt agactgtttt ttgataaaac
     6901 ctcttcagaa gactcctcta tatcgcctgt aaatcattta tttgaaagtt cattattttt
     6961 tttacttttt tttaattcaa aaatcatttt aatatctttc tctgaaaaaa aatttttcat
     7021 atctagatat atatcccaca aaaggtaatc ctcatcccta gtaatttcgt atatttttaa
     7081 cttctgttct aataatttta cccttgtttg tcaccattcc gaccatagcg atgatcctcg
     7141 ttcagaagaa ataatgtttc atacttctct agaaactcca cgagaatcaa gataagataa
     7201 cccagaggct tcttctgttt cttgataaat tcacttaacg ggttccattt tctctaatct
     7261 ctgttctagg tgttctattt gatcagttat tttgtttatt ttgtttgcat caactatagt
     7321 agacatcaga gatacggagc tattttcaaa atattctccc atagtagaat atttaatact
     7381 attctgcatc tcaagcatat tattaaacat atcttcctgt tctttgaagt agtcatctac
     7441 atccatactt ttttgaggtt cctgactgaa agcagattga atatctttat aaaattctcc
     7501 aacatttttg tatcatgctc tcttaaattc ctctatagtt ttgggatctt ttaattgatc
     7561 tatactaata gtttcaaatt ctttagcctt tgcttcatag tattcataca tcgttttata
     7621 tcatcaatcc tttaccaatt tttttaattt tattgtttct tcatggtttt gttctattag
     7681 accaaaatat tcttctacag agacattttt acatttctct gcattgatca tataatttaa
     7741 tactggtaaa atagtttcac ttcctatctg attttcaaaa tatgtgccac ttactcttat
     7801 ctcaagaaaa aaatcaagaa acttactaaa tactttattt tgtttatcta catcttttaa
     7861 tgtttctaac aattcttgta atttaggaat atttaataaa tcattaaatt cttgcatttt
     7921 gaaatctttt ctagcatcta tcactccttg tcttgttata agttcctgtc tatatatata
     7981 aaagtttgtt agtttctgat ttttattatt ttctgatgaa tagaatagag atcctctatt
     8041 accatctata gtaatatttt ctccaaaaca tacattagct cataactgta ttaactttat
     8101 gtcttcttta gaatagtaat ttacattacc gtcttcattt ctagctatca gtttatttac
     8161 ttcacttgaa atttttgcta tttttgcttc cattttgtct tgagtttcct ttaacatcga
     8221 tttgtattct ttaggaattt gtttaaccca atcattaact agctttattt cttcatctgt
     8281 taacttatct ttatctaaaa tactatttat atgattcctt tcttgttcac atttctcaac
     8341 ttctattttc tgtccagaaa gatgtaataa aatttcattt ggatttttaa aatcacttat
     8401 atggtacgat atagttttat ctaaaaagtt tactatgatt aatccatctt ctgcgaaaaa
     8461 gtttatccca acttctacaa tacttgattt attatcaatg tttttattta tataatcatc
     8521 aaacaaatct tctttttcct tattaataaa ttttactaaa ttttcttttg tttccaaaat
     8581 ctcaaataaa gtatctttat tactatcata agaaacttca acatctcaaa accatgtatt
     8641 tataagtaac ttatttcttt cataagatat actttctcaa tccaacaatc ataatcaccc
     8701 ttcttgataa aattgtagtc tattattaac taaatcattc accatacttt ctcacaatat
     8761 tccatctatt gttatatttg cattcttgtt ttgtaatgcc tgacataact ggtatacttt
     8821 aattgtattt atatttttcg aaatatctga aaatttctca ttttttagct tttctaaaat
     8881 ttctgtctgt atattttcag agttgaaatt atattgctct aaatttttct ctagttttgg
     8941 cactaatgtt tttgatacaa atttattgat atctttttct tttttaatca aattatcaaa
     9001 agatgtctct aaatctttta aagaaatatc ttgggcatta tcatcaaatt ttgcgatttc
     9061 ttttagtgat atatttacca taatatatta aatattatct aaaaggtcat ccaaatctcc
     9121 atctgctatt ttttcttcca tttcttcttc ctttattttt tgaatcaatt ctaaactttt
     9181 ttctattttt gttttttcat tttcttcctt tatttcgtct aaagactcct ttaaagctaa
     9241 tattaattta tccataattt cttctgtagc atattcactt tccaataaag ataaaaaacc
     9301 ctctgcgaga ggccgatatg ttttaatttt atttaacact tttatcagaa actctttttt
     9361 tctttccata ttagaaatta aataataaaa atatctaaca atatgattat atcccccccc
     9421 ccccc
//
LOCUS       DAOSID010000020         9117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_7220_length_9117_cov_11.777643, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000020 DAOSID010000000
VERSION     DAOSID010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 9117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9117
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7220_length_9117_cov_11.777643"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PK674_01660"
     CDS             <1..278
                     /locus_tag="PK674_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15271.1"
                     /translation="IRCYGLCEYWYGNREKGISFLEDSFSLHNKDTEVIYNLIEIYIL
                     EHKYQRAKNMIKHYHKNYNELVTVDKDISYYDSKIDLFEEFVKLQTV"
     gene            298..1209
                     /locus_tag="PK674_01665"
     CDS             298..1209
                     /locus_tag="PK674_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15272.1"
                     /translation="MKTKSVVARDVQRILKKSLKRIILTSIFLSFLFYLLNIFVGFSL
                     KIENIGEFVTSKVGIYFYINEENKGSDDIFKRILSIKDELNRHGIKAEFSSKEDAFNY
                     LETRVPAITKNFDKFGIENPLTSTLYVMFRNQQEYNIMKDIIVKNKDLILNIKDVDKG
                     ATLVQQENRSLKIIKVMNIIKFSFYFIVVMLGIIILSFTQHLLRHFFNNFYEEIEIKK
                     LLGAKTKDVNMGFIITLLLSITLGFIIGFILTCITLGILNKNLLALNLDLSLCSIAPR
                     LLLLYILFSLIAIILGYRMLKQKAKKF"
     gene            1265..1693
                     /locus_tag="PK674_01670"
     CDS             1265..1693
                     /locus_tag="PK674_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15273.1"
                     /translation="MKKFLENITNIACNLFILIIIGFGFYFLLMLKLINIDPNNLLNY
                     FGGNEADVALWNIRNILTPKFVIIIAIILLIGIGLFFRNFFTEKKSISKISLGIIIKS
                     IIMIIIIKFSRFILGLITDYTTHLENKEGANIEYNTNLYK"
     gene            complement(1861..2550)
                     /gene="rplA"
                     /locus_tag="PK674_01675"
     CDS             complement(1861..2550)
                     /gene="rplA"
                     /locus_tag="PK674_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L1"
                     /protein_id="HOG15274.1"
                     /translation="MSKKGKKYLASTAMIIKGKAYPLNEAVELVKKTSNVRFDATVEL
                     SVKTNANPKFNDQILRGTAVLPHGTGKTKKIAVFVLDEKLDEAKKSGADIYGSTDLLN
                     NIKNEKIDFDILITTTNSIRELAPVAKQLGPKGLMPSPKAGTVTNDLIKTVEEFKKGK
                     IEFKLDKTGNVHVPVGKVSFTENKLAENIQAILDTMEENKPAGVKGKLIKKVVISSSM
                     GPGILLEYIGK"
     gene            complement(2727..3170)
                     /locus_tag="PK674_01680"
     CDS             complement(2727..3170)
                     /locus_tag="PK674_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L11"
                     /protein_id="HOG15275.1"
                     /translation="MAKVQRILNLEVVAGKAQPAPPLGPMLGANGVNIGQFTKEFNEK
                     TQELMQKFGGFDVKVKCKLIVYVDRTFDLEIGTPVTSNLILRKIKEKKGSGEPNKNRL
                     AGKLTRADLEEIYELKKNDLNANDVEAGIKIIAGTARNMGVEVEM"
     gene            complement(3176..3892)
                     /locus_tag="PK674_01685"
     CDS             complement(3176..3892)
                     /locus_tag="PK674_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15276.1"
                     /translation="MNDNYHFESQTVRWAVIVLSVSLLLVYVVLYYVKGDLWKKDLNT
                     SQNTGDIVIKEIENGYPPENYSTGIEMETNTGINNLVGNQNITGTTQSSYIGINTTGT
                     SVTGTVLQDDDIENMIILSGTKTYYGELDFVDKMGISYSYVLIDQKGIYYLNMGGHKY
                     DFSDIARKLKGNLYVMNTDQELIANSLFGDKITFINIPEYKNKKVLFLLDINNQSWLL
                     AIDYKIYHQVKPYLKSLFIY"
     gene            complement(3919..4500)
                     /gene="nusG"
                     /locus_tag="PK674_01690"
     CDS             complement(3919..4500)
                     /gene="nusG"
                     /locus_tag="PK674_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOG15277.1"
                     /translation="MVQEQNIGEIRKQIQDNTFRWYVLSVVSGQEQLVIDNMKERIQK
                     QGLSEDVVDYLSPIVNEYSMRKGEKITKQKKLYPGYVFIKSRMNDKIWYIVRNTPGVR
                     LIVGAETRPIPLTDQEYEDMINHIKKSEERSSLKIPYKEGDVVMLKSGDFKGMKGNIK
                     TIDHDKGTVVVNIEMLGRLTPVAVDADKIELMS"
     gene            complement(4549..4731)
                     /locus_tag="PK674_01695"
     CDS             complement(4549..4731)
                     /locus_tag="PK674_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOG15278.1"
                     /translation="MMKFIYDSLDTIKGLKHPNKKMYINLTISIFAMVIVAGLYFIFA
                     DTIFSSIYNLFYSMAS"
     gene            complement(4986..5519)
                     /locus_tag="PK674_01700"
     CDS             complement(4986..5519)
                     /locus_tag="PK674_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15279.1"
                     /translation="MNLEKLFGSKTKVDILKYLLFRRQGVSMRALESELEWTFPAIKK
                     QVESLESANIIEIDKNSTGRSITLKAEVSQLLKELLYFGLKSELINLFSTYEFMIEKY
                     FFGKCFGINLDMDLVVIYKNLEKPQIDKIKESISEIFRGYFIEIVNVVFMSLDEWNKR
                     YRLADRFVLDIMRTRQE"
     gene            5701..5925
                     /locus_tag="PK674_01705"
     CDS             5701..5925
                     /locus_tag="PK674_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15280.1"
                     /translation="MAKKTKKKLRAYIGASIIIIGFMVFTAGKPNPINPKIIDPTWQT
                     MLLGTIMMFLGYKIGQRAFPALLEDEEDLN"
     gene            6018..6725
                     /locus_tag="PK674_01710"
     CDS             6018..6725
                     /locus_tag="PK674_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HOG15281.1"
                     /translation="MKILDLIFPKQCINCGKIGEYLCKDCKKQLKPHREMCSSCHKYS
                     NNYETCLDCKTAKEFFLDGILIPFSYTGFLKKIILKLKYYHKKDLVDFLIDRVVIAIY
                     ANNKLNNKLVQGNVVVSGIPSHWHRRYFVKGYNQSYLLAKNLSKKLGLEYKQFLKKTK
                     KTKSQASLDRAGRLQNLKNAFAFKGDFSFLEKTKTLILVDDITTTGATMNEIAKLIKH
                     KYPKISIWGVVLGRKDS"
     gene            6928..8667
                     /locus_tag="PK674_01715"
     CDS             6928..8667
                     /locus_tag="PK674_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15282.1"
                     /translation="MTTEKNLTNTNPSSNNQAGTTPANTNSNNEEILDSLDIDLDLTG
                     LDIQNENLKANDDMGEMEDENSQSDDIQKGNTTDEETKNLELDTPDSGEINSPKVETN
                     SDSADNDRLSQENKKFEESMGQYNINENIQKQKENIDLSDSQDLDLPFVGAENNVFKD
                     KINENTEQGVQLDSLSDSFAKEKVENNTTTEDQETTKNTNTQNIDEPESQDSNISSNK
                     EEEFQTNTGYIPNEDEFLQVQNLLNSNQTGNIDLSTLGHTTSHNNLTSGNLDQKTDTT
                     NQDEGLNLDEIISNPTTNHETINIDQIIQSSQFEPIVIKENKQIEQSMNLTDTKQQIP
                     AGTNVIPPVQVVNKKKKKGSGIKFFILILLLIVGGVMVLSKMYPEEIRQIITAIRDGN
                     DVDIEDYEDENMENLDMEETDEVDENLENKEEIDPNSLAGLIETENEENNDEQGIDSV
                     HDSANDDPNAFNPFEGIDTMIQDEMDSNQAIIEQLKEYAQLGDGFNIRGRENNNTTAI
                     KYGLYIKNHSERLLNSIENGEEINMLIVEEYIGKFDNYIQKLHTLTEGENSDDLNPQP
                     IQLDNEQPAQTES"
     gene            8639..>9117
                     /locus_tag="PK674_01720"
     CDS             8639..>9117
                     /locus_tag="PK674_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HOG15283.1"
                     /translation="MNNQHKQNLKFSLSIESLVEKFLYYLEYEKNNSPKTLENYSLWL
                     NRFINYIGDIDVTKIKAMHILDYRMYLNSLDLSKKTINYHIVAIRSFLKFCLKNDIDC
                     MSPEKLELSKIPNREINYLLEDEVEKIMQAPFLLKQNDLKRKRDSAILWMLYGTGLRV
                     "
BASE COUNT         3545 a         1343 c         1142 g         3087 t
ORIGIN      
        1 ttattagatg ttatggatta tgcgaatatt ggtattgaaa tagagaaaaa tgaattagtt
       61 ttctggaaga ttccttttca ttacataata aagacacaga ggtaatttat aatttgatag
      121 aaatatatat tttggaacat aaataccaaa gagcaaaaaa tatgataaaa cattatcaca
      181 aaaactacaa cgagttagtt actgtggata aagatattag ttattacgat tctaaaatag
      241 atttatttga agaattcgta aaacttcaaa ctgtttaatt taatataata ataaaaaatg
      301 aaaacaaaat ctgtagttgc acgagatgtt caaagaatat taaaaaaaag tttaaaaagg
      361 ataattctta cctctatctt tttatctttt ttgttctact tattaaatat ctttgttgga
      421 tttagtctaa aaatcgaaaa tataggagaa tttgttacaa gcaaagtagg aatatatttt
      481 tatattaatg aagaaaataa agggtctgac gatattttca aaagaatact aagcataaaa
      541 gatgagctaa atagacattg aatcaaagca gaattttcat ccaaagaaga tgctttcaat
      601 tacctagaaa ctagagttcc agccattaca aaaaactttg ataaatttgg gatagaaaat
      661 ccgttaacct ccacattata tgtgatgttc cgcaatcaac aagaatacaa tatcatgaaa
      721 gatattattg taaaaaataa agacctaata ttaaacataa aagatgtaga taaatgagcg
      781 accttagttc aacaagaaaa cagatctttg aagattataa aagttatgaa tataatcaaa
      841 tttagttttt actttatcgt ggtaatgttg ggaatcataa tattgtcttt tactcaacat
      901 ttattaagac attttttcaa taacttttat gaagaaatcg aaataaaaaa actactatga
      961 gccaaaacca aggatgtaaa tatgtgattt ataataacct tattactttc aataacatta
     1021 tgatttatta tatgatttat tttaacttgt ataacacttg gcatactaaa caaaaatctt
     1081 ttagcactca atttagatct aagtttatgt agtatagctc caagattatt acttttgtac
     1141 atcctgtttt ctttaatagc aataatttta tgatatagaa tgttaaaaca aaaagcaaaa
     1201 aaattctaaa tcatacgaca aatctgatca caagcttcaa tttcatatat taaaagttac
     1261 aaaaatgaaa aaatttttag aaaacatcac aaacattgca tgtaatttat tcatcctaat
     1321 aattatctga ttttgatttt atttcttact gatgttgaaa ctcataaaca tcgatccaaa
     1381 taatctatta aattattttg gatgaaatga agcagatgta gctttatgga atatccgaaa
     1441 catattaacc ccaaaatttg taataataat tgccataata cttctaatat gaatctgact
     1501 atttttccga aattttttta cagaaaaaaa gagtatttca aagatttcat tgtgaataat
     1561 aataaaatct ataataatga tcataataat taagttttca cgatttatat tgtgactaat
     1621 aactgattat acaacacact tagaaaacaa agaatgagca aatatagagt acaatactaa
     1681 cttatataaa taataacaaa aaaatctcca ttaattaaat acggagattt tttttacaat
     1741 acaaaaaatt cggcataatt aaaattttaa aaaaaactca aaaaatcaat ttttagtatt
     1801 ttttgctcgt gatatggaaa aagtctaaag aatatagtat tttatttaaa aattttacta
     1861 ctattttcca atatattcaa gcaaaattcc aggtcacatt gaggaagaaa taacaacttt
     1921 ttttattaat ttccctttta ctcaagctgg tttattttct tccattgtat ccaaaattgc
     1981 ttgaatattt tcagccaatt tattttctgt aaacgaaact tttcctactg gaacatgaac
     2041 attcccagtt ttatctaatt taaactctat ctttcatttt ttaaattctt caacagtttt
     2101 tatcaaatca tttgtaacag ttccagcttt tggactaggc atcaaaccct ttggtccaag
     2161 ttgtttagca acaggtgcca attctctaat actatttgtt gtagtaatca aaatatcaaa
     2221 atcaattttt tcatttttaa tattgttcaa caaatctgta gatccataaa tatcagctcc
     2281 agatttttta gcttcatcca atttttcatc taaaacaaat acagcaattt ttttggtttt
     2341 tcaagtccca tgtggtaata cagctgttcc tctgaggatt tgatcattaa attttgggtt
     2401 agcattcgtt tttactgata gctccacagt agcatcaaat cttacattag aagttttttt
     2461 tactaactcc acagcttcat ttagaggata agcttttcct ttgataatca tagctgttga
     2521 agctaaatac ttttttcctt ttttagacat tttaaataat ttaatttata aaagtggtat
     2581 tagcggttca aaaacctccc acaaaaatgc taggcactag acattagtcg ttagtcaata
     2641 gtcactagtc gatagtcgat agcttctagc tactgactaa cgactaatga ctaaagacta
     2701 acgactaacg aatagcgact ataaaactac atttcaactt ccacccccat atttctagca
     2761 gtgcctgcaa ttattttaat ccctgcttct acatcattag catttaaatc attctttttt
     2821 aattcataga tttcctctaa atcagctcta gttaattttc aagctagtct atttttgttt
     2881 ggttctcatg agcctttttt ttctttaatc tttcgtagta ttaaattgga agtaacagga
     2941 gttcatatct ccaaatcaaa agttctgtct acataaacga ttaatttaca ttttactttc
     3001 acatcaaaac ctcaaaattt ttgcatcaac tcctgtgttt tttcgttaaa ttcttttgtg
     3061 aactgaccga tattaactca gtttgctcac aacataggtc atagtggagg tgcaggttga
     3121 gctttcccag ctacgacttc caagttcaaa attctttgta cttttgccat cgtttttaat
     3181 aaataaataa agattttaaa taaggtttta cctggtggta tatcttataa tcaatagcta
     3241 acaaccatga ttgattgttg atatccaaca aaaacaaaac ttttttattt ttgtactcag
     3301 gtatatttat aaatgtaatt ttatcaccga acaatgaatt tgctatcaat tcttgatcag
     3361 tattcattac atataaattt cactttaatt ttcttgcaat gtcagagaaa tcatatttat
     3421 gacctcacat attcaaataa taaattcatt tttgatctat caaaacataa ctataagata
     3481 ttcacatctt gtccacaaaa tccagttctc catagtatgt tttagtccca gataatataa
     3541 tcatgttttc tatatcatca tcttgtaata ctgttccagt aacacttgtt caagtagtat
     3601 ttattcaaat atagctactt tgtgttgttc aagtaatatt ctgatttcca accagattat
     3661 ttattcaggt gtttgtttcc atttctattc aagtagagta gttttctggc gggtatcaat
     3721 tttcaatctc tttaattaca atatctcaag tgttttgact tgtatttaaa tcctttttcc
     3781 acaaatctca ctttacataa tacaaaacta cataaaccaa aagcaatgaa acagataaga
     3841 caattaccgc ccatctaaca gtttgagact caaaatgata attatcgttc attttgtttt
     3901 atttaaattt aaattttatt aactcatcaa ttcaatttta tctgcatcta cagctaccgg
     3961 ggttagtctt ccaagcattt caatatttac tactacagtt catttatcgt gatctattgt
     4021 ttttatattt catttcattc acttgaaatc tcaagatttg agcatcacaa catctcattc
     4081 tttgtacggt attttaagag aagatctttc ttctgatttt ttgatatggt ttatcatatc
     4141 ttcgtattct tggtcagtaa gcgggatggg acgagtttct gctcaaacaa tcaatctaac
     4201 cccaggtgta tttctcacaa tataccatat tttatcattc attctagatt ttataaatac
     4261 ataacctgga tatagttttt tttgtttggt aattttttct cattttctca tagaatattc
     4321 gtttactatt ggagacaaat aatctacaac atcttcggag agtcactgtt tttgaattct
     4381 ttctttcata ttatctatta ccaattgttc ttgtccagaa acaacactca aaacatacca
     4441 cctaaaagta ttatcttgta tttgttttct aatctctcaa atattttgtt cttgaaccat
     4501 ttttttgttt attttaatgt ataaaacaac ataataagca attaactatt atgaagccat
     4561 agaataaaat aaattatata ttgaactaaa tatagtatca gcaaatataa aatacaatca
     4621 cgccactatt accatggcaa aaatagaaat agttaaattg atatacattt ttttgttagg
     4681 atgttttaat cctttgatag tatccaaact atcataaata aatttcatca ttttaaattt
     4741 attttgcata ataaaaaaaa cttccaaaga aatatttaat gattgctagc tatatttcaa
     4801 gtgaaaatta actagagaaa aaaacagccc ctaaaacttg cacaattcca gattgcgaac
     4861 aatttcatcg aatagttatg gaagctactg attagaccct cagggaaaat ccagtcccaa
     4921 agacagcatt ccaaagacag cattcgggtg gtagaacaca taactttatt aacttttaaa
     4981 ctaagttact cttgtctagt tctcataata tccaaaacaa atctatccgc taatctgtag
     5041 cgtttattcc attcatccaa agacataaat acaacattga ctatttcaat aaaatatcat
     5101 ctaaatatct cactgataga ttctttgatt ttatcgattt gaggcttttc taaatttttg
     5161 tatattacta ctaaatccat atctaaattt atcccaaaac attttccaaa aaaatatttt
     5221 tctatcataa attcataagt agaaaaaaga tttattaatt ctgatttaag accaaaatat
     5281 agtaattctt tcaacaattg agaaacttcc gcttttaaag tgatagatcg acctgtgcta
     5341 tttttatcaa tttctataat gttggcagat tccagagact cgacttgttt tttgatagca
     5401 gggaaagtcc attccaattc actttccaaa gctctcatag aaactcattg tctcctaaac
     5461 aacaagtatt taagtatatc tacttttgtt ttgctaccaa aaagcttttc aagattcata
     5521 aaaatatatt agctaaataa acattactta taattcaaaa taagaattta ttagacaaat
     5581 acaaggaaaa attatttgtt taattataaa ctgaaataca caagaagaaa taaatgataa
     5641 atcgcttgaa tattggaatc atttaattaa acttaacttt gtcaacaaaa atttaaaaac
     5701 atggctaaaa aaacaaaaaa aaagctccgt gcttatatag gagcaagcat aattattatt
     5761 gggtttatgg tgtttactgc cgggaaaccc aatccaataa atcctaaaat tatcgatcct
     5821 acttggcaaa ctatgttgct ggggacgatt atgatgtttt taggatacaa aataggacag
     5881 agagccttcc cagctctcct agaggacgag gaggatctca actaacctat aaatcagagt
     5941 ctttatggct ctttttttct aatttgaaat ttattgaaaa atctatattt attcattatt
     6001 tactatccca tatctccatg aaaatactag atctaatatt cccaaaacaa tgtataaatt
     6061 gctgaaaaat atgagaatat ttatgtaaag attgtaaaaa acagctaaaa cctcatagag
     6121 aaatgtgttc atcttgtcat aaatattcaa acaactatga aacttgtttg gactgtaaaa
     6181 cagcaaaaga gttttttttg gactgaattc ttataccttt ttcttacact tgatttctaa
     6241 aaaaaataat tttaaaatta aaatattatc acaaaaaaga tcttgtagat tttttgatag
     6301 atagagtagt aatcgccatt tacgcaaata ataaattaaa taacaaactt gtacaatgaa
     6361 atgttgttgt ttctgggatt ccgtcccatt ggcatagaag atattttgtt aaatgataca
     6421 accaatctta cttactagca aaaaacctat ccaaaaaatt atgattggaa tacaaacaat
     6481 ttctaaaaaa aactaaaaaa actaaatctc aagctagttt ggatagggca tgaagattac
     6541 aaaatctaaa aaatgctttt gcttttaaat gagatttttc ttttttggaa aaaacaaaaa
     6601 cacttatttt agtcgatgat attaccacaa caggagccac tatgaacgaa atagcaaagc
     6661 taataaaaca caaatatcca aaaatttcta tttggtgagt agtactagga agaaaagatt
     6721 cataaaacta cttacaagtt ataaagttac aagtaaatag tttataaagt taaaagttgc
     6781 aagttataaa gttattaata ctttacaaac tttataaact ttccaacttt acaaactttc
     6841 taaactcatt aaaaaagtgg tcaaaaattt gcattatttt catttgtgat tataattaag
     6901 ttggttttat aatttaattt ttttaatatg actactgaaa aaaacttaac aaacacaaat
     6961 cctagttcta acaatcaagc atgaactaca ccagcaaaca ctaattctaa taatgaggaa
     7021 atcttggatt ctttagatat agatttagat ttaacttgat tggacataca aaacgaaaat
     7081 ttaaaagcca acgatgatat gtgagaaatg gaagatgaaa actcacagtc cgatgatatt
     7141 cagaaatgaa atactacaga tgaagaaaca aaaaatttgg aattggatac accagattca
     7201 tgagaaataa acagtccaaa agtagaaact aatagcgatt cagctgataa tgatcgatta
     7261 agtcaagaaa ataaaaaatt cgaagaaagt atgggtcaat acaatatcaa tgaaaatata
     7321 caaaaacaaa aagaaaatat agatctttct gatagccaag acttagacct accctttgtt
     7381 tgagcagaaa acaatgtttt caaagataaa ataaatgaaa atactgaaca atgagttcaa
     7441 ctagatagct tatctgattc atttgctaaa gaaaaagttg aaaataacac tacaacagaa
     7501 gatcaagaaa ctactaaaaa cacaaatact caaaatatag atgaaccaga aagtcaagat
     7561 tctaatatct cctccaataa agaagaagaa ttccaaacaa atacaggcta tatcccaaat
     7621 gaagatgagt ttttacaagt tcaaaatctt ttaaattcaa atcaaacagg gaatatagac
     7681 ctgtctacat tagggcatac cactagtcat aacaatttaa cttcatgaaa tttagaccaa
     7741 aagacagata caacaaatca agatgagtga ttaaatttag atgaaataat atctaatcca
     7801 acaacaaacc atgaaactat aaatattgat caaattatac agtcaagtca gtttgaacca
     7861 atagttataa aagaaaataa acaaatagaa caaagcatga atttaacaga taccaaacaa
     7921 caaattccag catgaacaaa tgtaattcca cccgttcaag ttgtaaataa aaagaaaaaa
     7981 aagggatctg gtatcaagtt ttttatttta attttgctgt taatagtttg atgagttatg
     8041 gtccttagta aaatgtatcc tgaagaaatc agacaaataa ttacagctat aagagacggg
     8101 aatgatgtag atattgaaga ttatgaagat gagaatatgg aaaatttaga tatggaagaa
     8161 acagatgagg tagatgaaaa cttagaaaat aaggaggaaa ttgacccaaa tagtttggct
     8221 tgattgatag aaacagaaaa tgaagaaaat aatgatgaac aaggtataga ttcagtacat
     8281 gattcagcaa acgatgatcc caatgctttc aacccttttg agggaataga tactatgata
     8341 caagacgaaa tggattcaaa tcaagcaatt attgaacaat taaaagaata tgcccaatta
     8401 ggagacggat ttaatattcg atgaagagaa aacaacaata ctacagctat caaatattga
     8461 ttatatataa aaaatcatag tgaaagactt ctaaatagca ttgaaaatgg tgaagaaatt
     8521 aatatgctca tagttgaaga atatataggc aagtttgata attatattca aaaattacat
     8581 acacttacag aaggggaaaa ctcagatgat ttaaacccac aaccaataca attagataat
     8641 gaacaaccag cacaaacaga atcttaaatt tagcttatct atagagtccc tagtagaaaa
     8701 atttctttat tacttggaat atgaaaaaaa caatagtcca aaaacattgg aaaattatag
     8761 tctatggtta aatagattta taaattatat ttgagatatt gatgtaacaa aaataaaagc
     8821 aatgcatatt ttagattata gaatgtatct caatagttta gatttatcaa aaaaaacaat
     8881 aaattatcat atcgtagcga taagatcttt tttgaaattt tgcctaaaaa acgacataga
     8941 ctgtatgtct cctgaaaaac ttgaattatc caaaatacca aatagagaaa taaactatct
     9001 actagaagac gaagtagaaa aaattatgca agcacctttc ttattaaaac aaaacgattt
     9061 aaaaagaaaa agagattctg cgatactttg gatgctttat ggtacaggtc tgcgtgt
//
LOCUS       DAOSID010000021         8692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_7602_length_8692_cov_15.431284, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000021 DAOSID010000000
VERSION     DAOSID010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 8692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8692
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7602_length_8692_cov_15.431284"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..845)
                     /locus_tag="PK674_01725"
     CDS             complement(165..845)
                     /locus_tag="PK674_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15284.1"
                     /translation="MLKGKFIVIDGTDGSGKGTQTKLLVQRLRDSGYNVEIADFPQYG
                     KKSAMMIEDYLNGEFGTAQEVGPYRASILYAVDRYAASFEIKKRLEEGKIIISNRYVS
                     SNMGHQAGKIKNPKERDEFLNWLEDLEYGIFDIPRPDKQILLYMPTEIGQKLVDKKGH
                     RNYVGGNKRDIHESDLQHLKDAAEAYKYVAKKYNRMIIDSAPNGNLLSIEEINNLIWK
                     EVMNILQV"
     gene            1221..4091
                     /locus_tag="PK674_01730"
     CDS             1221..4091
                     /locus_tag="PK674_01730"
                     /inference="COORDINATES: protein motif:HMM:NF024108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HOG15285.1"
                     /translation="MIKETKNISNIASLNEKLKGNSKYFLLQLKPFKDNELGVNHRDT
                     VLRNCASLKRKNISFILYGNNHNIKIFVRLPREFETFFKNTFYASFNTSDLEDTEIKP
                     FVSDKGRILEKKYIIFKKDAELNDKTFFTKDGSYMDPMRDILALFGNIASTDNLTIEF
                     QYKFKEVKNFFGYIKDFAKLVWGNNKNSEENGNQKELFLSIAYALDSKDSFSHKNIDT
                     NIKAALTQFGAKIKIKTAPKFHGMKFDQAINFFHVPTKENFVKGLEYTVYRKLPYPTN
                     LPTAKNTPESELTILGKTHYRGEAIEFGMKREDKFRHVYIVGKTGTGKSTFISNMVKS
                     DIEAGNGVCLLDPHGDLVDTVLEHIPSSRINDVILFDVSDTDYPIGFNLLQADNEDEK
                     NRIASGVVSTFQKLFEHSRGPRLEYILRNVVLSVIDYPNATLMHITRILVDKDFRDEV
                     MSSLTDPVLKKFWEGEFNKWNDRQRDEAIAPIVNKIGQFLSSKLVRNIFGQPRTRLNM
                     RKAMDEGKIILVNLSKGKIGEDNATMIGSLLVTKIQIDAMSRADIAREDRRDFYLYID
                     EFQNFATKAFATILSEARKYRLSLIIANQFTAQLQEEVRDAIFGNVGTIMAFTLGKDD
                     AEIISGQFKGIVSTNDLISLPMFTAYLKLMANGIASDPFSMRTNPLPSPEGSLELIDK
                     IRKQSRQRYAMERGELESLMDAWSNKTFSKQEKIQEKARLEGLGVSEKEYENSQDLSV
                     QSKIGLFKENKMDNVEPDAIVFDIKNNRHKLCRYEKVKNFDESLELKFQKGQILKAPN
                     GMEVKMHIDIYDNKNPEALQVWIGEKEDIKEQLEEAYKNNPNMKFVPNIGKLEKENPS
                     AEQAKPTYVQNGIVDNENNGSFSIKDIKLGNWYEGYIKLSYNYGMFITVKGVEGLLHK
                     NFIVAPEGVEWKKYYNIGDKIKVKAKEFKIINDEKKVVWSQK"
     gene            4570..5445
                     /gene="rsmH"
                     /locus_tag="PK674_01735"
     CDS             4570..5445
                     /gene="rsmH"
                     /locus_tag="PK674_01735"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOG15286.1"
                     /translation="MINHFPVMLNEVLESIPMNCKIVVDGTLGHGGHSRAILEKFPSI
                     KKLFGFDLDPNILEETKNRLKDLKNKIEFIGSSYVDIPKFLGGQKADFVLLDLGVNME
                     HFKDGERGFSLNYNSKLDMRFDNKNGKSAYDIINDYSEQELIRIFVDYAEFSIPKAQE
                     IARIIVRERKNKKIETTFELKNLLNQVGLGQKASTIIFQAIRIETNKEIDNLKLMLDQ
                     LPNVLSDGGRCGIITFHSLEDRIVKNYFKDLSKSGLFELVYKKAIPANYQEVLKNRAS
                     RSAKYRVIEKIFIKD"
     gene            5455..5826
                     /locus_tag="PK674_01740"
     CDS             5455..5826
                     /locus_tag="PK674_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15287.1"
                     /translation="MNKFVFISTNSIKEYYNFLLNKQKSHKFIVDTVKLSGLFVFLSL
                     CGFIYLRFVSLASTEGYFLRKANNELAAMQFKQEIVKTEILEQTQRNREKMYGNNQTK
                     KIIEIRPEIVQIPNKTELSYK"
     gene            complement(5837..6397)
                     /locus_tag="PK674_01745"
     CDS             complement(5837..6397)
                     /locus_tag="PK674_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15288.1"
                     /translation="MGNYRDEFLENTYYLISNKGFNNSILFENSDDYKTFILYTVTNI
                     LDHGGISISAYSILPNSFYFVIKNQEQGLKLSDFMRKIQVSYAMYFKKKSENKSSSKG
                     LPVFDGRFKAYKIELEELKEIESCVAFEPLRIGLVEDIKNWPYTSAHQSLDTGYNYQS
                     QMYLKIYSEIPQIQKTFFTEEFLDKI"
     gene            6642..7583
                     /gene="trxB"
                     /locus_tag="PK674_01750"
     CDS             6642..7583
                     /gene="trxB"
                     /locus_tag="PK674_01750"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOG15289.1"
                     /translation="MENVIIIGSGPAGHTSAIYASKALLNPLMFEGFMAGGIAAGGQL
                     TTTTIVENFPGFPDGIDGSQLMHNMRKQSINSGARIETKTVDKVDLSASPFKIYVGGE
                     MFEAKTVIIATGATAKRMGLLGEARYRGRGVSACAICDGGLPIFRDQHIIVIGGGDTA
                     MEEAIHLTHFASKVTVLVRRDELRASKIMQERAKSNPKIEFLWNTEAKEILGVEGGNM
                     TGLRIVNNKTNEESVLEAKGLFYAIGHTPNTSFLDGQLELDEAGYIITKPGSTQTSVP
                     GVFAAGDVQDKKFRQAVTSAGTGCMAAIEAEKYLNTL"
     gene            7742..8077
                     /gene="trxA"
                     /locus_tag="PK674_01755"
     CDS             7742..8077
                     /gene="trxA"
                     /locus_tag="PK674_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="thioredoxin"
                     /protein_id="HOG15290.1"
                     /translation="MGVIHLEGVEQFKKEILEFKGVVLVDFWAERCGPCRMLGPILEE
                     LNTENEGKNVKIVKINVENPENGPLARSFQVSSIPAVFVLKNGEVVKPIIGVNPKNVY
                     QEAIDQYLT"
     gene            8409..>8692
                     /locus_tag="PK674_01760"
     CDS             8409..>8692
                     /locus_tag="PK674_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="toprim domain-containing protein"
                     /protein_id="HOG15291.1"
                     /translation="MAKNLVIVESPAKSETIKKILGSDFEVKASYGHVVDLPQKSLGV
                     DVENNFKPIYEVGPDKKRTIADLKKYAKQAEKVWIATDEDREGEAIGRHVA"
BASE COUNT         3269 a         1114 c         1408 g         2901 t
ORIGIN      
        1 tcgcccgctc catttaaaaa acaacagaaa tgttggtttt tttattccca aaatactgtt
       61 ctccatcgtt taaataatta attatcccga tgttttatta ttaagaaatt tgtttatatc
      121 aaaaaaatga tagcactcca taaggttata aatttataac tatgctaaac ttgtaatatg
      181 ttcataactt ctttccaaat taaattattt atttcttcta ttgaaagtaa atttcagttt
      241 ggagcactat ctataatcat tcgattatat tttttagcaa catatttgta agcttctgct
      301 gcatccttta gatgttgtaa gtctgactca tgaatatccc ttttatttca tcaaacatag
      361 tttctatgtc attttttgtc tactaatttt tgtcaaattt ctgttggcat ataaagaaga
      421 atttgcttat ctggtctagg aatatcaaag attccatact ccagatcttc taaccagttc
      481 aaaaactcat ctctctcttt tggatttttt attttaccag cttgatgtca catgtttgat
      541 gaaacatatc tgttggaaat gataattttt cattcctcca atcgcttttt aatttcaaaa
      601 gatgcagcat acctatctac agcatacaaa atggaagctc tataaggtca tacttcttgg
      661 gcagttcaaa actcgccatt taaataatct tcgatcatca tggcactttt tttgccatat
      721 tgtgggaaat ctgctatttc tacattgtat cacgaatctc ttagtctttg aactaaaagc
      781 tttgtttgag ttcattttca acttcagtca gtaccatcaa tgacgatgaa tttacctttt
      841 agcattttta tgacgataaa aaataaaata aattatcttt aaatctgctc aaacaaatta
      901 aataatttaa ctaaatttag caattctttt cgcatatata tacacattta tactttataa
      961 tcaacagtta aatctaaatt tttaataatt aacccaagga catagatgaa atattttaaa
     1021 aatagttaaa cattatattt ttttattaat attataataa acttcataag tattttgtat
     1081 cctaacattt tatcttaatt tttcaagaat tatcctatag tgttctgccg ttatttacaa
     1141 cctacaaaaa atactattgc tatttgctag aaaaagtata taataatgtt gggttaattt
     1201 aaaacaaaaa tttaataata atgataaaag aaactaaaaa catatccaac atagcatcgc
     1261 taaatgaaaa acttaaaggc aattccaaat attttttgct acaattaaaa ccttttaaag
     1321 acaatgaact ttgagtaaat caccgagata cagttttgag aaactgtgct agtttaaaaa
     1381 gaaaaaacat atcgtttata ttgtatggaa acaatcacaa tataaaaatc tttgtcagac
     1441 tgcctagaga gtttgagact tttttcaaaa atacatttta tgctagtttt aacacttcag
     1501 atcttgaaga tacagaaata aagccgtttg taagtgacaa aggtcgtatt ttggaaaaaa
     1561 agtatattat ttttaaaaaa gatgctgaat taaatgataa gacttttttt accaaagatg
     1621 gatcttatat ggatcctatg agagatattt tagctctttt tggaaacatt gcatccaccg
     1681 acaatttaac tatagaattt cagtataaat ttaaagaagt taaaaacttc ttttgatata
     1741 taaaagattt tgctaaattg gtttggtgaa acaataaaaa tagtgaagaa aatggaaatc
     1801 aaaaagaatt atttttgtct atagcttatg ctttggatag caaagatagt ttttctcata
     1861 agaatataga tacgaacata aaggctgcac taactcaatt tggtgcaaaa ataaaaataa
     1921 aaactgctcc aaaatttcat ggcatgaaat ttgatcaagc aataaatttt tttcatgtgc
     1981 ctacaaaaga aaattttgtg aaatgattgg aatatactgt ttatagaaaa ctaccctatc
     2041 cgacgaattt accaacagct aagaatactc ctgaaagtga gttaacaatt ttagggaaaa
     2101 cgcattatag atgagaggcg atagagtttg gaatgaaaag agaagataag tttagacatg
     2161 tttatattgt atgaaaaact tgaacctgaa aatcaacatt tatttccaat atggtaaaat
     2221 ctgacataga agctgggaat ggagtttgtt tattagatcc tcacggagat ttggtagata
     2281 cagttctaga acatattccc agcagtagaa taaatgatgt gatattattt gatgtatcag
     2341 atacagatta tccaatagga ttcaatttgc tacaagcaga taatgaagat gaaaaaaata
     2401 gaattgcttc tggagtggtt tcaactttcc aaaagctatt tgaacatagt cgatgaccta
     2461 gattagagta tatactgaga aatgttgttt tgtcggttat tgattatcca aatgctactt
     2521 tgatgcatat tactagaata cttgtggaca aggattttag agatgaagtg atgagtagtc
     2581 tgactgatcc tgttttaaag aaattctggg aaggcgagtt taataaatgg aacgacagac
     2641 aaagagacga agctattgct cctattgtaa ataaaatctg acaattcctt tcttctaaac
     2701 ttgtaagaaa catttttgga caaccaagaa ctaggttgaa tatgagaaaa gcgatggatg
     2761 aatgaaaaat aattttggta aacttgagta aatgaaaaat atgagaagat aacgcaacaa
     2821 tgattggttc tcttttggtt acaaagatcc aaattgatgc gatgtctcgt gctgatatag
     2881 ctagagaaga tagaagagat ttttatcttt atatagatga gtttcaaaac tttgctacaa
     2941 aagcttttgc tactattttg tcagaagcta gaaaatatag attatctctg atcattgcaa
     3001 atcagtttac agctcaatta caagaagagg tcagagatgc tatttttggg aatgtgggaa
     3061 caataatggc ttttactctt tgaaaagatg atgcagaaat cattagcgga caattcaaag
     3121 gaatagtgag tacaaacgat ttgatctctc tacctatgtt tacggcatac ttgaaactga
     3181 tggcaaactg aattgcttct gatcctttta gtatgagaac caatcctcta ccaagtccag
     3241 aatgatcttt ggaattgata gataaaatta ggaaacaatc tagacaaaga tatgcaatgg
     3301 aaagatgaga attggaaagt ttaatggatg cctggagtaa caaaactttt tcaaaacaag
     3361 aaaaaattca agaaaaggct agattggagt gattgggagt tagtgaaaag gaatatgaaa
     3421 attctcaaga cctgtctgtt caatctaaaa tatgactgtt taaagaaaat aagatggata
     3481 atgttgaacc tgatgcaatc gtttttgata taaaaaataa tagacataag ttgtgtcgat
     3541 atgagaaagt taagaatttt gatgaaagct tggaattgaa atttcaaaaa tgacaaatcc
     3601 ttaaagcacc aaatggaatg gaagtaaaaa tgcacataga tatttatgat aacaaaaatc
     3661 ctgaagcatt acaagtttgg ataggtgaaa aagaggatat aaaagaacaa ttggaagagg
     3721 cttataaaaa taatccaaat atgaaatttg tcccaaacat agggaaattg gaaaaagaaa
     3781 atccatctgc agaacaagca aaacccactt atgtacaaaa tggaatagta gataatgaaa
     3841 acaatggatc atttagcatc aaagatatta aattaggaaa ttggtatgaa tgatatatca
     3901 aattaagcta taactatggt atgtttatca ccgtaaaatg agttgaagga ctactacata
     3961 aaaattttat agtagctcca gaatgagtgg aatggaaaaa atattataat atttgagaca
     4021 aaataaaagt aaaagcaaaa gaattcaaaa taataaatga tgaaaaaaag gtggtttgga
     4081 gccagaagta aacaaacatt agacttatca aactaataaa atatttataa tcctcatttg
     4141 tagactaact ccctaaaagg gagttttttg tttctccact tgtaacctca atgaaattac
     4201 ctcccaaaaa gctagttgac aaagatggag agaaagatat tcctgagaat aaatgtaggg
     4261 aaataaagca ataatcaaaa gaataaaaca tactataata tattacacga tctaatttga
     4321 aaaaactcat tcttcatcgt actcatattt atttgtcaat cattttggta tgtctataat
     4381 tatatttata atactttttt tctttatcat gataagctgg aatgtttttg tacaaattca
     4441 tgtttttatt ttaataaaga ttaaaattta gatggcactt attataagtt ataagaaaaa
     4501 aaagtttctt ttgcaatttt attttaattt tatagactga gtttgattta atatgctatt
     4561 tataagctta tgataaatca ttttcctgta atgttgaacg aagtattgga gtctataccg
     4621 atgaattgta aaatagtcgt agattgaaca ttgggacact gatgacattc aagagcgatt
     4681 ttggaaaaat ttcctagtat taaaaaactt tttgggtttg atttagatcc aaatattttg
     4741 gaggaaacta aaaatagact taaagatttg aaaaataaaa tagaatttat ttgatcttct
     4801 tatgtagata ttcctaaatt tttgtgaggg caaaaggctg atttcgtttt gctggatttg
     4861 tgagtaaata tggaacattt caaagattga gagcgtggtt tttctttgaa ttataattca
     4921 aaacttgata tgagatttga taataaaaat ggaaaatcgg cttacgatat tatcaatgat
     4981 tattctgaac aggaattgat aaggattttt gtagattatg ctgaattctc tattcctaaa
     5041 gcacaagaaa ttgctaggat tatagttaga gagaggaaaa acaaaaagat agaaactact
     5101 tttgaattaa aaaacttact aaatcaagtc ggtctgggac aaaaagctag tactataatt
     5161 tttcaagcaa ttaggattga aactaataaa gaaatagata atctaaagtt gatgttggat
     5221 caacttccaa atgttttgtc tgattgatga agatgtggaa tcattacatt tcatagtttg
     5281 gaagacagaa tcgtaaaaaa ttattttaaa gatttatcca aatcaggctt gtttgaattg
     5341 gtttacaaaa aagctattcc agcaaactat caagaagttc taaaaaatag agctagtaga
     5401 agtgcaaaat acagagtaat agaaaaaata tttataaaag actaatctga ccctatgaat
     5461 aaatttgttt ttatatctac aaattcaata aaagagtatt ataatttttt gttaaacaaa
     5521 caaaaatcac ataaattcat cgtagatacg gttaagttat cttgattgtt tgttttttta
     5581 tcgctatgtt gatttattta tctgcgcttt gtttctttag catctactga atgatacttt
     5641 ttgagaaaag ccaataatga gctagcagct atgcaattta agcaagaaat agtaaaaaca
     5701 gaaattttgg agcaaaccca aagaaatcga gaaaaaatgt atgggaataa tcagacaaaa
     5761 aaaattatag aaatccgtcc agaaatagta caaattccta acaaaacaga actaagctac
     5821 aaatagtatt ttttcattaa attttgtcta aaaattcttc tgtgaagaag gttttttgta
     5881 tttggggaat ttcactatat atttttaaat acatttgaga ttgataatta tagcctgtat
     5941 ccaaagattg gtgtgcacta gtatatggcc aattttttat atcttcaact aatcctattc
     6001 taagaggttc aaaagctacg caactttcaa tttccttgag ttcttctaac tctattttgt
     6061 aagccttgaa tcttccatca aaaacaggaa gtcatttgga tgatgattta ttttcgcttt
     6121 ttttcttgaa gtacatagcg taacttacct gtatttttct catgaagtcg gataatttaa
     6181 gcccttgctc ttgatttttt attacaaaat aaaagctatt tggtaatata ctataagcag
     6241 agatacttat tccaccgtga tctaatatat tggtgacagt atataaaata aaagttttgt
     6301 aatcatcaga attttcgaat aatatactat tgttgaatcc cttatttgaa attaaataat
     6361 aagtattctc caaaaactca tcacgatagt ttcacatttt taatctaata attagataaa
     6421 aaacttaaac ttttaaatac tctatgttgg gaacagtata ttaaaaaaaa gaatctaatc
     6481 aagaatattt atttgacaaa cttaaatatt attgaaaaat aacaattaaa taatttttta
     6541 tttttttggt tatgaaatat atttcgtctt gttgattttc ttgcaaaaat atatattttt
     6601 aatagagata ctttataaat ttattttata attatttttt tatggaaaat gtaattatta
     6661 tatgatctgg ccctgcatga catacctctg cgatatatgc aagcaaagcc ttattaaatc
     6721 cacttatgtt tgagtgattt atggcctgag ggatagcggc atgatgacaa ctaacaacta
     6781 ccacaattgt tgaaaatttt ccctgatttc cagatggtat agactgatca caactgatgc
     6841 ataatatgag aaaacaatct ataaattctt gagcaagaat agaaaccaaa accgttgaca
     6901 aagtagattt aagtgcaagc ccgttcaaaa tatatgtgtg atgagaaatg tttgaagcta
     6961 aaactgtgat tatagctact ggagctactg caaaaaggat gtgacttttg tgagaagcac
     7021 gctatcgagg aagaggagtt tccgcgtgtg ctatttgtga ttgaggttta ccaatatttc
     7081 gtgatcaaca tataattgtt atttgaggag gagatactgc tatggaggaa gcgatccatt
     7141 taactcattt tgcatccaaa gttactgtat tggtcaggag agatgaattg agggcctcca
     7201 agataatgca agaaagggca aaatcaaatc caaaaataga atttttgtgg aatacagaag
     7261 caaaagaaat tctaggagtg gaaggttgaa atatgacttg attgaggatt gtgaataaca
     7321 aaacaaatga ggaatctgta ttggaagcaa aatgactatt ttatgctata tgacatactc
     7381 caaatactag ctttttggat ggtcaattgg aattagacga ggctggttat ataattacaa
     7441 aaccttgaag cactcaaact tctgttccat gagtatttgc ggctggagat gttcaagaca
     7501 aaaaattccg tcaagccgtt acctctgctg gtactggttg tatggctgca attgaagcag
     7561 aaaagtactt aaatacactt tagtcgctat ttgacagtcg ttagtcgaca gtcaatagtc
     7621 aatagtcaat agtcgatagt caacagctaa tagctaacga ctaatgacta gcgactatta
     7681 ggcgactagc gactattagg cgactagcga ctaatctttt aatttataac cttttaatat
     7741 catgtgagta atacatttag agtgagtaga acaatttaaa aaagaaattt tagaatttaa
     7801 gtgagttgtg ttagttgatt tttgggcaga acgatgcgga ccttgtagaa tgctttgacc
     7861 gattttggaa gaactaaata cagaaaatga atgaaaaaat gtaaaaattg taaaaattaa
     7921 tgtagaaaat cccgaaaatt gaccattagc tagaagtttt caggtaagtt ctattcctgc
     7981 tgtttttgtc ttaaaaaatg gagaagtggt aaaaccgata attggagtta acccaaaaaa
     8041 tgtttatcaa gaagctatcg accaatattt gacttagtta tttgaataat aagtttatat
     8101 tgaataaata agtttataaa acaaaaaatt ctgaataaat ttcagttttt ttgtttgata
     8161 atttttaaga agattagcat ataaaaccaa tctttatcta tattttctgt ctattttaac
     8221 aactcaaaat gtctattaag agtaattttt tagaaaaagt attaaaataa aggtgtaatt
     8281 gtatggtgtt gaaaggcatt tagctgtttt tgatttttat aaatattctt gtccttgaaa
     8341 ttaatgatgg catttgtaac atacaaggta tttatggaac gaaaatattt ataaacaaaa
     8401 ctaatctaat ggctaaaaat ctagttatag tggaatcccc cgcaaaatca gaaactatca
     8461 aaaaaatact tggtagtgat tttgaggtaa aagcatcata ctgacatgtg gtggatttac
     8521 ctcaaaaaag cttatgagtg gatgtagaaa ataattttaa accaatatac gaagttggtc
     8581 ccgataaaaa aagaactatt gctgatctaa aaaagtatgc aaaacaggct gaaaaagtat
     8641 ggattgcaac tgatgaagat cgcgaatgag aagccattgg acgacatgtt gc
//
LOCUS       DAOSID010000022         8259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_8085_length_8259_cov_14.301804, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000022 DAOSID010000000
VERSION     DAOSID010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 8259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8259
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8085_length_8259_cov_14.301804"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PK674_01765"
     CDS             <1..534
                     /locus_tag="PK674_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="GTP-binding protein"
                     /protein_id="HOG15292.1"
                     /translation="MKIRNFCIIAHIDHGKSTLADRMLEITSTIRKSNKNQVLDRMDL
                     EQERGITIKLTPVRMQRKGYELNMIDTPGHVDFQYEVSRSLAAVEGAILLVDSSQGIQ
                     AQTLSTLYQAIDQNLEIIPVLNKIDLPAANPERVAQEIENVIGIPKEEIFLVSGKTGE
                     GVEAVLDAIISKIPEPK"
     gene            711..1274
                     /locus_tag="PK674_01770"
     CDS             711..1274
                     /locus_tag="PK674_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15293.1"
                     /translation="MKYIDKILESGNTVFKANEIALLLDIENKNTLKSILQRFKKAGI
                     IIYHGLGLRSLKKYNPYELASKIKSKSYISLETVLQKEGVIFQDYENTVTLISDKSFE
                     QKIDTKIYSFYKIKDTILLNTIGIKDMGKYMMATKERAICDRIYLTPSYYFDNLSNLN
                     IKLLEKLSTIYNNSTNLRIKKLINELK"
     gene            1261..1980
                     /locus_tag="PK674_01775"
     CDS             1261..1980
                     /locus_tag="PK674_01775"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOG15294.1"
                     /translation="MNLNKKITSNNEEHKKIMFLIIKDLFSSDIKSQIAFKGGTLCMF
                     LYQLDRFSVDLDFDIIKPNVSLEKIKKTTKDILNQYGNIIEETKTKLLLKYDQKQIPL
                     KVEFNTRIYKNNTYEVKNFFGNSIIAMTLDCIFANKLVALTERKEQQNKIASRDLYDI
                     RFFFNNRWNINEAIIKERTGESLKTYLHSLLSFIPDNFNEENILRGIGELITEKQKYF
                     IKNKLIQETLNHIEFKISNLS"
     gene            2058..2564
                     /locus_tag="PK674_01780"
     CDS             2058..2564
                     /locus_tag="PK674_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="nitroreductase family protein"
                     /protein_id="HOG15295.1"
                     /translation="MQTLEAIKTRRSVRSYSDTQKLTPEQIAEVVEYGLYAPSAHNQQ
                     ARKFFIVTKQKDLDFLGELMQWGKMIPKSSGVVLACFDDSKLKSPDFIQQDMGASIQN
                     ILLAIHDKGYGAVWVGTYPRENLVDEIKKYFDFPTNIIPFAILAIGVPNEEFMPKNLK
                     VDGKIEIL"
     gene            2792..>3045
                     /locus_tag="PK674_01785"
     CDS             2792..>3045
                     /locus_tag="PK674_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15296.1"
                     /translation="MLENISKALIFDCVYDPYKGVVAYVKIIQGSFKTGDNLYLIHSE
                     ISLQATEVGYFSPEYTKDSQLTKGQIGYIVTGLKSVRDAQ"
     assembly_gap    3046..3145
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3146..4231
                     /locus_tag="PK674_01790"
     CDS             <3146..4231
                     /locus_tag="PK674_01790"
                     /inference="COORDINATES: protein motif:HMM:NF018161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15297.1"
                     /translation="KKSKPFVYAGVYPIETPDYDKLKDSLEKLSLNDSSIEYSMEDSK
                     ALGLGFRCGFLGMLHMDIIKERLSREFDLETIFTTPTVVYLVKSKTPALDKIKSGANV
                     TELAKTGYYRPILLINGISQEEVDQTKDFSIGEIIEKYGSILKLRMIVKAGSDIKEQG
                     IIDEIYEPIGEVEIVGPQEYTGEIMSLCQSYRGEMQNMEHIDQTRVVRKYLLPMGEII
                     VDFYDRLKSATKGYATMNYEFKRYQKSDLVKLDIYLNGEKIEALSMIIHKDNSAYQGR
                     DIVKKLKELIPRHMFVIPIQAGIGNKMIARENISALKKDVIAKCYGGDISRKRKLLQK
                     QKEGKKKMKAMGSVNVPSDVFIKMVTK"
     gene            complement(4258..5703)
                     /gene="amrB"
                     /locus_tag="PK674_01795"
     CDS             complement(4258..5703)
                     /gene="amrB"
                     /locus_tag="PK674_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOG15298.1"
                     /translation="MTKKKIIRFIVLCLVYLLIFVLFLWNKKKYHINFAVVPHFTIQE
                     QVVHRQYKNIQDTYQLSGKKINILIISPDHFYTLKNNNLKLNNIDKDMCYKNTCLNVK
                     GVYEGNGRKYKKHIKEHGLGEHFPYIKKYFPDSNLYLAKIKGRDFGRMNNVLEEFKKL
                     EKKGNLLVLASVDFSHYVSEDWANLHDIKSKYIMQNSLDKDDYKNIEVDCPTCLYLTN
                     LWASEKNQFPNFVFRDSSSVINNKDLGYDNTSRQWYLYGDEKENNNGITIGFFGSSLS
                     DLLSLNNEELVNHMRDFYQLGNIKNNPKYNVHRKGFGIDFLGFTLENSYSGLSSKLKT
                     LSELGFNIINLDLLKNIELNDNYGYFGNKGNANNIILEKNIRGIKIAFHGYDFRNREN
                     EDCKNLENYKSSGYINIVSVHGKEKINLLEDLIDCGTDVILIEQKKLSKNFDNNIQWY
                     KDKPIIFIAKNKYVLIDIDQSGTITIGEKNL"
     gene            complement(5705..>8259)
                     /locus_tag="PK674_01800"
     CDS             complement(5705..>8259)
                     /locus_tag="PK674_01800"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=25
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HOG15299.1"
                     /translation="MKKITLISLLLAGIFIFGCSQNGDNNQINTEVDTQITNSGFSSL
                     SDKLQKYKFSWKMGKPSTKEIKNLNYFWDGFHETWNKKVGKEVLEVEGWDFSECEEIT
                     LFDMNGTFEKQVSMDQIKKGNIELSGKYNGYGLGFQLDDNQLKFLDKHGWIVFNPGDR
                     RNLWSSEVGVLEQEERGLYYQMIGGNKLPYQRYPYHTVLITSDMLLHFYHKIFSNSLR
                     YYEESVARNTMQNLSEKMFSKFVDLYQTNKNKELTPYYAFSVAYWAIPYSILVPQNII
                     ETAIEEKMNNQDFYGGNMGKDIETEDLHNIILARLDTIQDKIPAEYQSAVKSALNEVL
                     KATSNRAEDPMLSALGLNVDKKIFIEQDYTQFTPRGHYTDSSLLKIYFIGMKRFMREK
                     LYFRDIEQAKISLIMINNIKDDELSNFNKLYDFIGKLIGQDDDVNVNDIQSYIKKNSW
                     NTDKDIIAGTNETHQEELQKLKPQKIISTHYKTETRGEVSESQAKDETAGFVFFGEKF
                     TIDSFIFDKMTAGSAEVEFAYKPNLQTTFIVPDALINTGIIRDVVDLWMERAKTKYNV
                     QQEQIDGYNQAKSEITNELNEFDFNISTYHQWLDSLSWLFALTDPNLPYFMQDPLYQY
                     KELNTFQGNYSELKHDTILYVKQAYAELGGGGEDECSIMITPPDLPVPKGYVEPNIDL
                     IDSILKLTNDTKDFFSNDEAYLEYAKFLLFIRDIAIKQSQNELISDEDFEKLRLYYDD
                     IINILYPKKVIDGDNDFISALIADIFTSENNGPLYIATGRPYLMIVNVKDANGARAVV
                     GPVYSTYEFYDSDEPIAREQGRYTDDDRQKGYDTLNHKAIYTIPMEKLLEHK"
BASE COUNT         2996 a         1123 c         1048 g         2992 t
ORIGIN      
        1 atgaaaatta gaaatttttg tattatagca catatagacc attgaaaatc tactttggcg
       61 gacagaatgc tggaaattac tagtacaatc agaaaatcaa ataaaaatca agtattggat
      121 cgcatggatt tagaacaaga aagatgaatt acaatcaaac taactccagt tagaatgcag
      181 cgaaaatgat atgaattaaa tatgattgat actccaggtc atgtagattt tcagtatgaa
      241 gtgtctcgtt ctctggctgc agtagaatga gcaattttgt tagtagattc aagtcaatga
      301 attcaggcac agacactttc tactctttat caagcgatag accaaaatct agaaatcatt
      361 ccagttctaa ataaaataga tttaccagca gcaaatcctg aaagagtagc acaagaaata
      421 gaaaatgtta tttgaattcc caaagaagaa atatttcttg tttcatgaaa aacaggggag
      481 tgagtagagg ctgttttaga tgctattatt tccaaaatcc cagaaccaaa ataaaatctt
      541 tcaagataat tttaaaataa caaataaatc tgatttttta gtttcagata catacgaata
      601 ctattaaaat tcgaatacta tcaaaattcc acaaaaaagt ttaattttct attgcaaaaa
      661 taaatattat gactatatag tatttatatt taaataccat agaggcataa atgaaatata
      721 tagataaaat attagaatca ggaaatacgg ttttcaaggc aaatgaaatt gctttattat
      781 tggatattga aaacaaaaat acattaaaat ctattctaca aagattcaaa aaagcgggta
      841 ttatcattta tcattgatta ggtttgcgaa gcttaaaaaa atacaatcct tatgaactag
      901 catccaaaat aaaaagcaaa tcatatattt ctttagaaac agttttacaa aaagaatgag
      961 taatatttca agattatgaa aatactgtta ctctcatatc agataaatca tttgaacaaa
     1021 aaattgacac caaaatatat tctttttata aaatcaaaga tacaatttta ttgaatacaa
     1081 taggcatcaa agacatgggt aaatatatga tggctaccaa agaaagagca atatgtgaca
     1141 gaatttatct tactccatcg tattattttg ataatctttc taacctcaat ataaaattat
     1201 tagaaaaatt aagtacaata tacaataata gcacaaattt acgtattaaa aaattaataa
     1261 atgaacttaa ataaaaaaat aacatcaaac aatgaagaac ataaaaaaat aatgtttttg
     1321 ataataaaag acttattttc ttcagacata aaatctcaaa tagctttcaa atgaggtact
     1381 ttatgtatgt ttctttatca actagacaga ttctccgtgg atttagattt tgatattatc
     1441 aaaccaaatg tttctctaga aaaaatcaaa aaaactacca aagacatctt aaatcaatac
     1501 tgaaacatca tcgaagaaac aaaaaccaaa cttttactga aatatgacca aaaacaaata
     1561 cccttaaaag tagaatttaa tacaagaatc tataaaaata atacttacga agttaaaaat
     1621 ttttttggga atagtatcat tgctatgact cttgattgta tttttgctaa caaattagtt
     1681 gctctcacag agagaaaaga acaacaaaat aagattgcct ctagagatct ttacgatata
     1741 cgattttttt tcaataatag atggaatata aatgaagcga tcattaaaga aagaacaggt
     1801 gaaagtctaa aaacctattt acacagtctc ttatcgttta ttccagataa tttcaatgaa
     1861 gaaaatattt taagatgaat tggagaactc atcacagaaa aacaaaaata cttcataaaa
     1921 aacaaattga tacaagaaac tttaaaccat atcgaattca aaatatcaaa tttgtcataa
     1981 ttttgacaag ccttatatct tttgaataca tatagaattc cgaatcaata ttttatatga
     2041 ttaaatattc aatcaaaatg caaacacttg aagcaataaa aactcgtaga tctgttagga
     2101 gttattctga tactcaaaaa cttacaccag aacagatagc tgaagtagtt gagtattgat
     2161 tatatgctcc atcagcccac aatcaacagg cacgaaagtt ttttatcgtc acaaaacaga
     2221 aagatttaga ttttttaggt gagttaatgc aatggtgaaa gatgattccc aagtcatcat
     2281 gagtcgtttt ggcttgtttt gatgatagta agctaaaaag tccagatttt attcaacaag
     2341 atatgtgagc tagtattcaa aatattttac tagcaataca tgataagtga tattgagctg
     2401 tttgggtatg aacatatcct cgtgaaaatt tagttgatga aataaaaaaa tattttgatt
     2461 tcccaacaaa tataattcct tttgcaatat tggctatatg agtaccaaac gaagaattta
     2521 tgcccaaaaa tctaaaagtt gattgaaaaa tagaaatttt atagattatt ttctgctcta
     2581 attcattttt atttggttcg taaagagtct tatatagctc cctatgattt atttaatttt
     2641 tcaaaataga agtccacgga tgatttcttc tttttaaaag gatttcttta ttttaataaa
     2701 ttaatatgtt tcacaaaaat attgaatatt cagaggattt cagtaataat cttatattga
     2761 tgaatttatt tttaataaga aaaatcgttc tatgttagaa aatatttcaa aagctttgat
     2821 ttttgattgt gtttatgatc catataaatg agttgtagcc tatgtaaaaa ttatccaatg
     2881 aagtttcaaa acatgagata atttatattt aattcactct gaaatctcat tacaagctac
     2941 tgaagttgga tatttttcac cagaatatac aaaagattct caacttacaa aatgacaaat
     3001 atgatatata gttacatgac taaaatcagt tcgtgatgct caaatnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnaaaaa atctaaacca tttgtttatg ctggagttta
     3181 tcctattgaa acaccagatt acgataagtt aaaagatagt ttagaaaagt tatcattgaa
     3241 tgatagttct attgaatatt ctatggaaga ttccaaagca ttatgattat gatttaggtg
     3301 tggttttcta tgaatgttgc atatggacat tatcaaagaa agattatcta gagagtttga
     3361 tttagaaact attttcacca ctcctacagt agtttatcta gtaaaatcaa aaactccagc
     3421 tttggataaa ataaaatctt gagcaaatgt gacagaatta gctaaaacat gatattatag
     3481 accgatattg ttaattaatt gaatatccca agaagaggtc gatcaaacaa aagatttttc
     3541 tatttgagaa attatagaaa agtattgaag tattttaaaa ttacgaatga ttgtaaaagc
     3601 ctgatcagat ataaaagaac aatgaataat agatgaaatt tatgagccaa tttgagaagt
     3661 agaaatagtt tgaccgcaag aatatacagg agagattatg agtttgtgtc aatcttatag
     3721 gtgagaaatg caaaacatgg agcatataga tcaaactaga gttgtacgaa aatatttact
     3781 cccaatgtga gaaattattg tagactttta tgataggtta aaatcagcta caaagtgata
     3841 tgcaacgatg aattacgagt tcaaaagata tcaaaaatca gatttagtaa aattagatat
     3901 ttacttaaat tgagaaaaaa tagaagcatt atctatgatt atacacaaag ataattcagc
     3961 atatcaatga agagatatag taaaaaaatt aaaagagtta attccaagac atatgtttgt
     4021 aatcccaatc caagctggga tttgaaacaa aatgatagct agagaaaata tttcagctct
     4081 taaaaaagat gtaatagcaa aatgttatgg ttgagatatc tctcgtaaaa gaaagttact
     4141 tcaaaaacag aaagaatgaa aaaagaaaat gaaagctatg tgaagcgtga atgttccatc
     4201 agatgtattt atcaaaatgg taacaaaata gttttgtcat aaagtaatta ttgaaagcta
     4261 cagatttttt tctcaaatag taatcgttca agactgatca atatcgatta atacatactt
     4321 attttttgct ataaatatta ttggtttatc tttgtaccat tgaatattat tatcaaaatt
     4381 tttgcttaat tttttttgtt caattaaaat tacatcagta ccacaatcta tcaagtcctc
     4441 taataaattt attttttctt ttcaatgaac actcacaata ttaatatatc agctagactt
     4501 gtaattttct aaatttttac aatcttcatt ctctctattt ctaaaatcat atcaatggaa
     4561 agcaatcttt attccacgaa tatttttttc taaaataata ttgtttgcat ttcacttgtt
     4621 tccaaagtaa ccgtaattat cattcaattc aatatttttc aacaaatcca aatttattat
     4681 attaaatcat agttctgata aggtttttaa tttacttgat aagccagaat aagaattttc
     4741 taatgtaaat ccgagaaaat caattcaaaa tcactttcta tgtacattat attttggatt
     4801 gttttttatg tttcataatt gataaaagtc cctcatatga ttcaccagtt cttcattatt
     4861 taatgataat agatcactta aagaacttca aaaaaatcat attgttattc aattgttatt
     4921 ttctttttca tctccatata aataccattg tctggaagta ttatcatatc ataggtcttt
     4981 attgtttatt acactactgg aatctctaaa tacaaaattt ggaaattggt ttttttcaga
     5041 agcccaaaga ttagttagat acaagcaagt agggcagtca acttctatgt ttttgtaatc
     5101 atctttatcc aaactatttt gcattatata cttgcttttt atatcatgta agtttgccca
     5161 atcttcactc acataatgag aaaaatctac agaagctagc accaataagt ttcatttttt
     5221 ttctaacttt ttgaattctt ccaaaacatt attcattctt ccaaaatctc ttcattttat
     5281 tttggctaaa tacaaattag aatctggaaa atattttttg atatacggga aatgttctca
     5341 taaaccatgt tcttttatat gttttttata ttttcgtcca tttcattcat aaactcattt
     5401 tacatttaag catgtgtttt tataacacat atctttgtca atattgttga gttttagatt
     5461 attatttttt aatgtgtaaa aatgatcagg acttattatt aatatgttaa tttttttccc
     5521 agaaagctga taagtatctt gaatattctt atactgtctg tgtaccactt gttcttgaat
     5581 agtaaaatgt ggaactacag caaaattgat atgatatttt tttttgttcc ataaaaacaa
     5641 tacaaaaatc aacaaataca ctaggcatag tacaataaat ctaataatct ttttttttgt
     5701 catattattt atgttcaaga agtttttcca taggaatagt atatatggct ttgtggttta
     5761 atgtatcata tcatttttgt cgatcatcat ctgtatatct tcattgttcc ctagctatag
     5821 gctcgtcgct atcataaaac tcataagtac tatatactgg tcatacaaca gctctagctc
     5881 agttagcatc ttttacattt acaatcatca aatatggtct tcatgtagca atgtataaag
     5941 gtccattatt ttctgaagta aatatatctg ctatcaaagc ggatataaaa tcattatctc
     6001 agtctataac ctttttagga tataatatgt ttataatatc atcataatat aatcttaatt
     6061 tttcaaaatc ttcatcactt attagctcat tttgactttg tttgatagcg atatctctta
     6121 taaataataa gaattttgca tactctaaat atgcttcatc attagagaaa aaatctttag
     6181 tatcatttgt taattttaat atagaatcta tcaagtctat gtttggttct acatatcatt
     6241 ttggtactgg taaatctgga ggagttatca ttatagaaca ctcatcttct catcctcatc
     6301 ataattcagc ataagcttgt tttacataca atatagtatc atgtttcaat tcagaataat
     6361 ttcattggaa agtatttaat tctttatatt ggtacaatgg gtcttgcatg aaataaggta
     6421 gatttggatc agtaagtgcg aataaccaag ataaggaatc caaccattga tggtaagtag
     6481 aaatattgaa atcaaattca tttagttcgt ttgtgatttc gcttttagct tgattatatc
     6541 aatcaatttg ttcttgttgt acattatatt ttgttttagc tctttccatc cataaatcta
     6601 caacatctct gataatacca gtatttatca atgcatcagg tactataaaa gttgtttgta
     6661 agtttggctt atatgcaaat tctacttctg cactaccagc agtcatctta tcaaaaataa
     6721 aactatctat agtgaacttt tcaccaaaga acacaaatca tgctgtttca tctttagctt
     6781 gagattcact aacttctcat cttgtttctg ttttgtaatg agttgatatt attttttgtg
     6841 gttttaattt ttgtaattct tcttgatgag tttcatttgt tcaagctatt atatctttgt
     6901 cagtattcca tgaattcttt tttatgtatg attgtatgtc gttaacattt acatcatcat
     6961 cctgtcatat taattttcct ataaaatcat ataatttatt gaagtttgat aattcatcat
     7021 cttttatgtt gtttatcatt ataagtgata tttttgcttg ttcaatatct ctaaaatata
     7081 atttttctct cataaatcgt ttcataccta taaagtatat tttcaaaaga ctgctatctg
     7141 tataatgtca tctaggagta aattgagtat aatcttgttc tataaatatc tttttatcta
     7201 cattaagtcc taaagcgcta agcattggat cttcagctct atttgatgta gcttttaata
     7261 cttcatttag agcagatttt actgctgatt gatattctgc tgggattttg tcttgtattg
     7321 tatccaatct agctaaaatt atattatgta aatcttctgt ttctatatct tttcacatat
     7381 ttcatcagta aaaatcttga ttattcattt tttcttcaat tgctgtttca attatatttt
     7441 gtggaaccaa tatagaatat ggtatagccc aatatgcaac agagaaagca taataaggag
     7501 ttagttcttt atttttattt gtttgatata aatctacaaa tttagagaac attttttctg
     7561 ataagttttg catagtattt ctagcaacac tttcttcata gtacctcaaa gaattagaaa
     7621 atattttatg ataaaaatgt aataacatat cacttgtaat taatactgtg tgataaggat
     7681 atctttggta aggaagtttg tttcatcata tcatttgata atacaatcat cgttcttctt
     7741 gctccaatac tcaaacttca gaagaccata aatttcgtct gtctcatgga ttaaacacaa
     7801 tccatcaatg tttgtctagg aatttcaatt gattgtcatc tagctgaaat cccaatccat
     7861 atcaattata ttttcatgat aattctatat tacctttttt tatttgatcc atagatactt
     7921 gtttttcaaa agttcagttc atatcaaata atgtaatttc ttcacattca gaaaaatccc
     7981 atcattctac ttccaacact tcttttcaaa cttttttgtt ccatgtttca tggaatcagt
     8041 cccagaaata gttgagattt tttatttctt tagttgaagg ttttcccatt ttccaactaa
     8101 acttgtattt ttgtagcttg tcagataagg aagaaaatcc agaatttgta atttgtgtat
     8161 ctacttccgt gtttatctgg ttgttgtccc cattttgact acatccaaaa ataaaaatac
     8221 ctgcaagtaa aagagaaatt aaagtaattt ttttcattt
//
LOCUS       DAOSID010000023         7706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_8734_length_7706_cov_14.723696, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000023 DAOSID010000000
VERSION     DAOSID010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 7706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7706
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8734_length_7706_cov_14.723696"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(366..1190)
                     /locus_tag="PK674_01805"
     CDS             complement(366..1190)
                     /locus_tag="PK674_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15300.1"
                     /translation="MGLCKYCGEDAGFLKKKHKECSILHDVNKNRIGKMIYFGFFGDE
                     DMTEIKEKVINLAKKSYITPKELDKLYISVYNKIVKQFLYDGIITHEEELRLTKFKKV
                     FNFDQEFLDQHGLFQKFIKALVVRDVANKELPNVDFESDFDLPFKLEKGEKIIWLFDN
                     VELYEKKKKKKSKHIRNTFDLEDELFFSSTFSNEFIKTKGMTYLNIGQFVITNKKNYF
                     VYDNDKYIEVRIKNLANLFPYQDGFGLKPKDKTGSLQVFKNLDGWFAYNVIFNLNR"
     gene            complement(1610..2827)
                     /locus_tag="PK674_01810"
     CDS             complement(1610..2827)
                     /locus_tag="PK674_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15301.1"
                     /translation="MNKINHFFRYTKSTNNYFQRHGKRYLFGTLGAFAIVTIALLFVG
                     FFSTLDLGKSSAQGEEGTTLTSGNIVSMGCATGATSCDLSNQGITSIASDAFVNHRDL
                     RGLYLSQNSLTSIFGVAFPNSLVNLDLAFNQITSLSGIAFPSSLQILNFSSNQITSIS
                     GVQFPDSLKILLLGYNKLTSVEQIVFNTESVLEELNLANNEISSIGEGIMGLTSLGDG
                     DGLNVHNNHIYYTQLSSGLLDFIDLKHILVQGDWRKTQTISGGENTCTFSDYTSMQTG
                     HSVAEQVLESNNLGYTCELGKGGADVQITVQAFPLIGPAHNQIPASGVKLKHISRPNI
                     TSGDCTLTGELPSLFTDISSPQTLFKKNYGMVCEISASTQLQVTIPADLPSGIYSGDI
                     VFTISEDGIVGST"
     gene            complement(3164..4465)
                     /locus_tag="PK674_01815"
     CDS             complement(3164..4465)
                     /locus_tag="PK674_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15302.1"
                     /translation="MNKINHFFRYTKSTNNYFQRHGKRYLFGTLGAFAIVTIALLFVG
                     LFSTLDLGKSSAQQSPPQVVLTSGNVVSMGCATDATSCDLSNKGIISIKNDAFVNHRD
                     LIELSLSQNALTGIFGVAFPNSLVDLNLAFNKITSLSGIAFPSSLQNLNIASNRLTSL
                     SGIAFPSFLQYLNLYSNGLTNLSGAVLPSSLLRLNLSFNLLKNIDQVVSLSSLQSLDL
                     SLNQLNSMPENTTQLINLQNDGGLNINNNHINYTQLSSGLLAFIDQKSKTGRRETQTF
                     TPPSSSGTYNKKITIEILGGGGSSCSFSGYSAMEATTSIASMELESETSAYYCTIGGK
                     SGSYVTVQSSPLISLEHNQIPASNVSMRQVLTGVSPVEGCSIVPQLGTTYCNIASGVT
                     LFSKQGAGACGYGNETQLKIIIPQSTPAGTYSGDVVFTINE"
     gene            4815..4888
                     /locus_tag="PK674_01820"
     tRNA            4815..4888
                     /locus_tag="PK674_01820"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4849..4851,aa:Gly,seq:gcc)
     assembly_gap    5044..5143
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5215..6600)
                     /locus_tag="PK674_01825"
     CDS             complement(5215..6600)
                     /locus_tag="PK674_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15303.1"
                     /translation="MKTTSSNPQTYRKDKINKINEFIDKMPTENENLKKKTRRGLFKE
                     LLQDPLIQEAQKERRLDRMTSLTPEKLERMNQLFLQEEFESTKEKTQAFVYETEEEYD
                     TRVNKTIAKMEEEFEKKKENLSESELKLLKNYAGIEDGKVMRYKEQVEEQKKYINDPN
                     LKKCFIEIGNPTQKWLYNKTTSEFISLPSGGRHKSLADTLETSKTDEVVGGYLQIDPV
                     EKIITIIGTSKDFGGTVEDFPSHACYILEKQFPGYLFSKIDIEVWSHGDCNGILDINK
                     YFGDLVCYGSIEDCELLFHYAKMADSEFVNDNYKYTIGNFLEAYLNLLDKDRKEMPGV
                     YPHSVALVKQDVNIKTEINSIEGNEFIEKVRNTGISMNGQVYGSVWAYYCNKLIKNIR
                     QHNTKKIYFVDIFNELKSAAYKTYSYRIKNNKSNNSAKKYVSDPIGGMNSISNSYNNI
                     IKDARHRPSKH"
     gene            complement(6853..7122)
                     /locus_tag="PK674_01830"
     CDS             complement(6853..7122)
                     /locus_tag="PK674_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15304.1"
                     /translation="MENFDLYHEPCEYYEPASMEDFYLYEDYDSALCPNSSFLKTEAG
                     KTEYGWRNKLLDLGQSTQKELSEIEKRREEMLKNGNEELRKALMC"
     gene            complement(7168..>7706)
                     /locus_tag="PK674_01835"
     CDS             complement(7168..>7706)
                     /locus_tag="PK674_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15305.1"
                     /translation="LYFFVPTILLLGVVTYYGKDQIIHRQYSNTGHIKEVFLAIEKIK
                     SSWFWGLGAASAGPASHHLPDGQAYNPENQYLQIWLEYGLIVFLGWMFLYIYLHLIGF
                     WAWKENWIEKKGTKDIKNMSLVLLAFSLGIFGLSIEGMVLHSFVDRMIVYPFVSLFAV
                     YYANYHKNTLSQKLYGLL"
BASE COUNT         2572 a         1194 c         1057 g         2783 t
ORIGIN      
        1 ttagtatgat actaacgttt ttcttttaaa tatttttttt cccggacact actgattatt
       61 tctattgcaa aataaaatat ttttcatatt ttatttcgtg gaaaattttt gatttatttt
      121 ctacaaaaaa ggagcaaaat gccttgcaaa aaaactacaa ctgcaaaaaa gcctaaagct
      181 aaaaaagcac ctgctaaaaa agcaaaaaag taatttattt aaaaaacgat taaaataaaa
      241 aacccgagta atcgggtttt tttgcatttt atagttaagt tacaagccaa aagcctgcct
      301 gccccgtaaa gttacaagtt gaaggtttgt aatacttgat gaactttata acttttctct
      361 acagtttatc tatttaaatt gaatattaca ttgtaagcaa accatccgtc aagatttttg
      421 aaaacctgta aagaacctgt tttgtcttta ggtttaagtc aaaatcaatc ttgatatggg
      481 aataaattgg ctaaattttt aatacgcact tctatatatt tgtcattatc atagacaaaa
      541 tagttctttt tatttgtgat gacaaactga ccaatattta aataagtcat cccttttgtc
      601 ttgataaatt catttgaaaa agttgatgaa aaaaatagtt catcttctaa atcaaatgta
      661 tttcgaatat gtttagattt tttctttttc tttttttcat ataattctac attatcaaat
      721 aaccaaataa ttttttctca cttttccaat ttaaaaggta gatcaaagtc agattcaaaa
      781 tctacattag gtaactcctt atttgccaca tctcttacta ccaatgcttt gataaatttt
      841 tgaaataatc aatgctggtc caaaaactct tgatcaaaat taaaaacctt tttaaattta
      901 gttagtctca attcttcttc gtgagtaatt attcagtcat acaagaattg ttttactatt
      961 ttattgtata cagaaatata cagtttatct agctcttttg gtgtgatata agattttttt
     1021 gctaaattta ttaccttttc tttgatttct gtcatatcct catctccaaa aaatccaaaa
     1081 tatatcattt ttcaaattct atttttgttt acatcgtgta aaatagaaca ttctttgtgt
     1141 ttttttttca aaaatcaggc atcttctcaa caatatttac ataatcccat tgcattaaaa
     1201 gttaatatat aaattttctg atcattgaac tttctgatca accatattat aattattttc
     1261 ttttatttgt caaatttttg agaacttttt ttgtgaattg ttaagcccaa tttcagctaa
     1321 caaaatataa agtaaacaaa aaaactaaaa ttataaaaag atttaatgtt tttggttagt
     1381 aaaagtttga aaaaaaacct attaaaacaa ataatcttga taagagatgg gtgaataaat
     1441 tagtgtaaaa aaaacttaaa agaaataaga acttatgacg gttatgcttg caagtaaacc
     1501 cctcaagagc caaatgaacg ctcgagatta ttttacagca aaatcatcaa gcaaaattct
     1561 cttgctcggt gattgataat aaaatatgta tatttgttta aaattaattc tatgtacttc
     1621 atactatacc atcctctgat atagtaaata ctatatctcc actatagatt ccgcttggta
     1681 aatctgcagg aattgtaacc tgtaattgag tagaggctga tatctcacat accataccgt
     1741 agtttttttt aaataaagtt tgtggagatg aaatatctgt aaataaactt ggtagttcac
     1801 cggttaaggt acagtctcaa ctagtaatat ttggtctact aatatgttta agttttactc
     1861 ctgaagcagg aatttgattg tgtgctggac caattaaagg gaatgcttga acagttattt
     1921 gtacatcagc acctcctttt cctaactcac atgtatatcc tagattattg gattccaaca
     1981 cttgttcagc aacagaatga ccagtttgca ttgaagtata atcagaaaag gtacaagtat
     2041 tttctcatcc actaatagtt tgagtttttc tccaatctcc ttggaccaaa atatgtttta
     2101 gatctataaa gtctaataaa cctgaagaca attgtgtata atatatatgg ttattatgaa
     2161 catttagtca gtctccatct cataaacttg ttaatcccat aattccttct catatgctac
     2221 ttatttcatt attagcaaga tttaactcct ccaagacact ctcagtatta aatactattt
     2281 gttccacact tgttaatttg ttataaccaa gcaataaaat ttttaaagaa tcaggaaact
     2341 gcactccgga tatgcttgtt atttggttac tagaaaaatt taatatttgc aaagagctag
     2401 ggaatgctat tcaggatagg cttgttatct gattgaaagc aaggtctaaa ttaactaggg
     2461 aattgggaaa tgctactcaa aatatgcttg ttagtgaatt ctgggaaaga tacaatcctc
     2521 ttaagtctct atgatttaca aaggcatcac ttgctatact agtaattcat tgattggata
     2581 aatcacaaga agtagcacca gtagcacagc ccatggatac aatatttcca ctggttagtg
     2641 ttgttccctc ctctcattgt gcagaacttt tacccaaatc caatgtgcta aagaatcata
     2701 caaatagtaa tgcaattgtt actattgcga atgctcataa tgtcccaaag agatacctct
     2761 ttccatgcct ttggaaataa ttattggtac tctttgtgta tctaaagaaa tgattaattt
     2821 tattcatttt ttattgaaat agaaagataa aaactttttc atattcaatt ataattaaaa
     2881 aaaaaatcct gtcaaatttt tgaggacttt ttttggtggc tataatcaag taacaagtgt
     2941 aaaaaaacaa gacaacaaaa aaaataaaat aagacaacca tatttactta aaaaattaaa
     3001 aaatatcaat ttttttcatt gttttttaga aaactacttt atttttgtta ttgtgtattt
     3061 tggtattaat atttattggt atagttattg ttccatttta accataaatg tagcaaaaaa
     3121 actctatgaa cttgttcaag cgtttattga tattaaattt ttattattca ttaatagtaa
     3181 ataccacatc tccactataa gttcctgctg gtgtactttg aggaattatg atttttaatt
     3241 gagtttcatt tccatatcaa catgctcccg ccccctgctt actaaacaag gttactccac
     3301 ttgcaatgtt gcaataagtg gttcctaatt gaggtactat actacacccc tctactggac
     3361 taactccagt caaaacttgt ctcatactta cattggaagc gggaatttgg ttatgttcta
     3421 agctaattaa aggagatgat tgaacagtta catatgatcc agattttcca cctatagtac
     3481 aataataagc agaagtttca gattccaatt ccatactagc tatactggta gtagcttcca
     3541 ttgcagaata gccagaaaaa gaacagctgg atcctcatca tcacaaaatt tctatcgtaa
     3601 tctttttatt atatgttcaa gaactagaag gtggggtaaa ggtttgagtt tctctccgac
     3661 ctgtttttga tttttgatct ataaaggcca aaagcccaga agataattga gtataattga
     3721 tatggttatt gttaatattt agtcatcaat cgttttgtaa atttattaac tgagtagtat
     3781 tttctggcat tgaatttaat tgattaagac tcagatctaa actctgcaaa gaactaagag
     3841 atactacttg atctatgttt tttagaaggt tgaaagacaa atttaatctc agcaaagaac
     3901 taggaagcac cgctcaagaa aggtttgtta atccattgct gtaaagattt aaatattgca
     3961 aaaagctagg gaatgctatt caggataggc ttgttaatct gttgctggca atatttaaat
     4021 tctgcaaaga gctagggaat gctattcagg ataggcttgt tatcttattg aaagcaaggt
     4081 ttaaatcaac tagggaattg ggaaacgcta ctcaaaatat gcctgttagt gcattctggg
     4141 aaagagacaa ttctattaag tctctatgat ttacaaaggc atcgtttttt atgctaataa
     4201 ttcatttatt ggataaatca caagaagtag catcagtagc acagcccatg gatacaacat
     4261 ttccactggt tagtaccact tgtggtggtg actgctgtgc agaactttta cccaaatcca
     4321 atgtgctaaa aagtcataca aatagtaatg caattgttac tattgcgaat gctcataatg
     4381 tcccaaagag atacctcttt ccatgccttt ggaaataatt attggtactc tttgtgtatc
     4441 taaagaaatg attaatttta ttcatttttt attgaaatag aaagataaaa actttttcat
     4501 attcaattat aattaaaaaa aaaatcctgt caaatttttg aggacttttt ttgtagacta
     4561 caatcaagga aaatacctaa caaacataaa aaatacaaat tgtatttttt ttacgactta
     4621 attactatct aaaaaacttt atcaaataat tatttactgt taaattcctt atctactaac
     4681 taaactaaac gatggtgtaa aatcaaaaat acaaaaaaat attagacatg tgtttttgtt
     4741 tgaaaatgaa atataaaatc ttcttgaaat taacaaataa atttacatac attaagttga
     4801 tggggaattt gtctgcgtgt gtagctcaat tggttagagc gtatccttgc caaggatgag
     4861 gttgcgggtt cgagtcccgt cacgcgctaa tcttgaaaaa aaacctgaaa tggttttttt
     4921 aattttaact gaaaaatatt gttgtgacta gttgtagggg ctagagcgtc ccgacctgtc
     4981 gggaaggctg cgggttcgag tcccgtcacg cgctaatctt gaaaaaaaac ctgaaatggt
     5041 tttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttttgat actaaaaaat
     5161 gaataaaaaa aacgccccaa aaggagcgtt aggggttgga aagggaaata aaaattagtg
     5221 tttagaaggc ctatggcgtg catctttaat aatattatta taagaattag atatagaatt
     5281 cataccacca ataggatcac ttacatattt cttagcagaa ttatttgatt tattattttt
     5341 tattcgatag ctataagttt tatacgctgc gctcttcagt tcgttgaata tatctacaaa
     5401 ataaattttt tttgtattat gctgtctaat attttttatg agcttattgc aataatatgc
     5461 ccaaacacta ccatatacct gtcaattcat tgatatgcca gtgttacgaa ccttttctat
     5521 aaattcattt ccctctattg aatttatttc cgtttttata ttaacatctt gctttacaag
     5581 agcaacagaa tgaggatata ctcaaggcat ttcttttcgg tctttatcaa gcagatttaa
     5641 ataggcttct aaaaaatttc caattgtata tttatagtta tcgtttacaa attcgctatc
     5701 tgccattttt gcataatgaa aaaggagttc acaatcttct atactaccat aacagactaa
     5761 atctcaaaaa tatttattta tatctaaaat accattacaa tctccatgcg accaaacttc
     5821 aatatctatt ttactaaaca gatatcaagg aaattgcttt tctaagatat agcaagcatg
     5881 acttggaaag tcttcaaccg ttcatcagaa atcttttgat gttcaaatta ttgtaatgat
     5941 cttttctact gggtctattt gtagatatcc cccaaccacc tcatctgttt tagatgtttc
     6001 taatgtatca gcaagagatt tatgtcttca tccagaaggt aatgaaataa actcagaagt
     6061 tgttttatta tataaccatt tttgtgtggg atttcctatt tcaataaagc attttttaag
     6121 attgggatca ttgatatatt ttttttgttc ttccacttgt tctttatatc tcataacttt
     6181 tcaatcttca atgcctgcat agttttttaa taattttaac tctgattcag ataaattttc
     6241 tttctttttt tcaaactctt cttccatttt agcaatagtt ttattcactc ttgtatcata
     6301 ctcctcttct gtctcatata cgaaagcttg tgttttttcc tttgtacttt caaattcctc
     6361 ttgcaaaaag agttgattca ttctttccaa cttttctggt gttagagaag tcattctgtc
     6421 taagcgcctc tctttttgag cttcttgaat aagtggatct tgaagaagct ctttgaatag
     6481 tcatctcctt gttttcttct tcaaattttc attctctgtt ggcattttgt caataaactc
     6541 gttaattttg ttaatcttgt cctttctgta agtttgtggg ttacttgaag ttgttttcat
     6601 tttttataaa ttaaataata aaaattgttt aatgcaagta taaccccccc cccaatttgt
     6661 caaatttttg atgacttttt tttgaatctg atttgacttt ttttgttttt ggtatgaaaa
     6721 aagggatgta attttaaaaa aaacctggaa cgaaggtttt ggtttgggat atctttaaac
     6781 ttacaaagaa aatatatgag tttgagttat tgaaaactaa gaaactaact ttaaattttg
     6841 tctgcaaaaa aattaacaca taagtgcttt tctcaattct tcatttcaat ttttgagcat
     6901 ttcttccctt cttttttcta tttcagataa ttctttttgt gttgactgcc ccaaatccaa
     6961 aagcttattt ctccacccat attctgtttt tcaagcttct gtttttagaa aagaagaatt
     7021 aggacaaagc gctgagtcgt aatcttcata taaatagaaa tcttccattg atgctggctc
     7081 ataatattca cacggttcat gatacaaatc aaaattttcc atttttatta aaagaaaata
     7141 aatacttatt gaacaataaa tatttcctta caatagtcaa tacaattttt gtgataatgt
     7201 gtttttgtga taatttgcat agtagacagc aaataggctt acaaaaggat aaactatcat
     7261 cctatctaca aaactatgta gaaccattca ttctatactt agtccaaaga ttcataaaga
     7321 aaaagctaaa agaaccaaag acatattttt gatatcttta gtcccttttt tttcaatcca
     7381 attttctttc caagcccaaa atcatattaa atgtaaataa atatacaaaa acatccatca
     7441 taagaaaaca attaatcaat attctagcca tatttgtaaa tactggtttt ctggattgta
     7501 ggcttgtcaa tcgggtaaat gatgagaggc tggaccagca ctagccgctc atagccccca
     7561 aaaccaagaa cttttgattt tttctatagc taaaaacacc tctttgatat gtcatgtatt
     7621 ggagtactgc ctatgaataa tttgatcttt cccataataa gtaactactc caagtagtaa
     7681 tattgtagga acaaaaaagt ataaaa
//
LOCUS       DAOSID010000024         7607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_8877_length_7607_cov_13.766022, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000024 DAOSID010000000
VERSION     DAOSID010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 7607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7607
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8877_length_7607_cov_13.766022"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1544)
                     /locus_tag="PK674_01840"
     CDS             complement(129..1544)
                     /locus_tag="PK674_01840"
                     /inference="COORDINATES: protein motif:HMM:NF010335.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOG15306.1"
                     /translation="MQIVKGCGSVLDQYFAENTMSIHIKENNPLLKKNGGPGGRKPEL
                     GSKVLDKKFVEKLIDDIFKEVETRHDSILEIDRNLSKIIQLGPYRIVIVYPPLSDGLE
                     ITVVKPIKKLSLDDYKIEQEVLDWFKTSAKGILVSGAPGSGKTTFAQALVEFYERDKK
                     IIKTIESPRDLMVPENVVQYSFTHGSHDEVRDILLLSRPDYTVYDEVRNKPDFELYKD
                     LRLTGIGLIGVIHATRPIDSIQRFLGTIEMGIIPQVLDTVMFIDKGEIKEILQLHLTV
                     KVPAGMQSEDLARPVIEVSSFFDKEVVFEIYTFGEQIVVIPLGEDIFSQERPSKMDEY
                     AKIAIDKQLQELMPCSFISKIKGGTVYLFVPKSEKGAIIGRAGKNIQALEEEIGLRIE
                     LETFDDLPLLDIDVKIEKHGNTIVIYFPKHYVERNVYIMIGKDLLHTQTNDHAQLIIK
                     NKNYVKMIGSKGFVLVDYESI"
     gene            2506..3291
                     /locus_tag="PK674_01845"
     CDS             2506..3291
                     /locus_tag="PK674_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15307.1"
                     /translation="MILFKQINNIYIFLSIFVFILSVLYIYIFRILSNKPPTEDQIKE
                     ISVRRILAGERVLKEINKNRDFSNKMYSFVAGFPSFVKYGLEFANTLIILILIYLYFQ
                     NALSLTGNIEQMFYRLVTAGFIANVYLLKRLNYTSILQRLLTFLVINFAIYISLFSAF
                     SGDIGSIVFLGIIRNILSTMMVFHVHRTKVGEYLRKMDYLFWIFTTMLALVVNIVLLF
                     HTDLAGGLLFPIILLYVGIQGISLYYSIRYINKIKEVTEEDEL"
     gene            3690..4193
                     /locus_tag="PK674_01850"
     CDS             3690..4193
                     /locus_tag="PK674_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15308.1"
                     /translation="MKTLIKILSITLFLLAIFGISFAQHGKWGNWGSDPMQIFNTFVD
                     SGSNEGNYNIHETALDGVTDQQGGYARSFKITNTLDYIRIYMDPYLQRTIYAGLVLAV
                     VALIYLGFLLVTGGLHKEGDRSKIKSKVMYIVVGVFLLSGFYFIIKVMVALVTSLFGG
                     STGDSGY"
     gene            4228..5193
                     /locus_tag="PK674_01855"
     CDS             4228..5193
                     /locus_tag="PK674_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15309.1"
                     /translation="MKLLKYFLFIIGVLGFLLVQNKVGAAVGIPPSFRNNFTDYLTSS
                     EPDKEGRVENVIDLGIDTNKSLKQNIKCLFYPNASPVVGCETTVAGGKIWDVARYIGF
                     AILVIFLVVVGINLLLQGGNPEKARSSLKSLIYILYGSVLFFGATWILGSSLGIENVK
                     GTEGLVDALQGGPDSLFFKIISTLKALAFFVAIVMVVVYGFKMMSVSDQATKTKTAIK
                     GIINVIIALVIIKVIDYVYYIAQLPNFTTQATEFIIEVAKILGFIIGAVLVLLVFYSG
                     FLFLTDQGKSENMKKAKNIIVGVILGAIVIFMLLLIMYQLFAEFA"
     gene            5233..6003
                     /locus_tag="PK674_01860"
     CDS             5233..6003
                     /locus_tag="PK674_01860"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HOG15310.1"
                     /translation="MKVICSIGGGEIGGFFNGKKRPIETKVIDHELITISGKKSPKVL
                     FIPTASGDNKSYISNFTNYYTSLGCDVNTLLLHNKLIDPITLKKKVFSADIIYVGGGN
                     TLKMLNLWKKVGLISLLQQAHKKGIIFAGISAGGICWFHGGMSDSRKFSDKDTGFIIV
                     KGLDFVPLYFCPHFDRDVKRKEILPEFIKRTNKKRIVLEDGTAFLVRGKRWRIVKSKP
                     EARACLGFHHKDEVIYQALEVTDFQSLSKLSRSYPLFI"
     gene            6343..>7607
                     /gene="aspS"
                     /locus_tag="PK674_01865"
     CDS             6343..>7607
                     /gene="aspS"
                     /locus_tag="PK674_01865"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HOG15311.1"
                     /translation="MLLSGELSKHVGEIVEIRGWLHTVRVVSANLAFLILRDRDGLAQ
                     VVFEDANEIKKLEGCLLGTILSAKGTVAEMPKGKFRFELQKASVNVIRKVVYPSPIDI
                     SKDEIHADGETLLENKVVALRHPKQMKIFKLAAIIEKNMRAFFEQNGFTQINSPKIIG
                     FPTEGGAEVFEIDYFGRKAYLAQSPQFYKQMMVPVFERVYEIGRAYRAEKSNTSRHLS
                     EILMLDMEMGFIDSFDDIIEMTEKFANFVVKQTWTEAEHLLTALGATKPLLVDQFPRI
                     SVDDLHELMLKETGEDYRKEKDLAPSEEKFICEYSAKHWNSDFVIVDGFPWSDAKFYH
                     HQNKDNPNIADRADLLFRGVEIVTLTQREVNYQKLVDQIKAQGQNPEHPGLKHYLDSF
                     KYGMPDEGGFGLGITRFIQKLIGLENIKEA"
BASE COUNT         2585 a         1104 c         1135 g         2783 t
ORIGIN      
        1 aatctaatta tactcatttc ttaaaaaacg aacttaattg cagcaattaa tacaagtttg
       61 taaagtatta ataactttca acttgtaact ttacaaactt tcaacttgta actttataaa
      121 ctcattattt atatgctttc ataatccact aaaacgaatc attttgatca aatcattttt
      181 acataatttt tattttttat tatcaactgt gcatgatcgt ttgtttgagt gtgcaataag
      241 tcttttcata tcattatata cacatttctt tctacataat gctttgggaa ataaattact
      301 atcgtattac catgtttttc tattttgaca tctatatcca acagcggtaa atcatcaaaa
      361 gtttctaatt caattcttag tcaaatttct tcttccaatg cttgaatatt tttccctgct
      421 cttcatataa tagctcattt ttctgatttt ggtacaaata aatatactgt acctcacttt
      481 attttggata taaaactaca aggcatcagt tcctgcaact gtttatcaat agctatttta
      541 gcatactcat ccatttttga gggcctttct tgagaaaaaa tatcctctca aagtgggatc
      601 actactattt gttctccaaa agtataaatt tcaaacacga cttctttgtc aaaaaaactg
      661 gaaacttcaa taactggcct agctaaatct tcagattgca tacctgctgg aacttttaca
      721 gtaagatgta attgtagaat ttctttgatt tcacctttat ctataaacat aactgtatcc
      781 aaaacctgtg gaataattca catttctatt gtccccaaaa atctctgaat actgtcaata
      841 ggcctagttg catgtatgac tccaatcaat ccaataccag taagtctgag atctttgtat
      901 aactcaaaat ctggtttatt tctcacttca tcatagacag tatagtctgg cctagaaagc
      961 aataaaatat ctctaacttc atcgtgtgat ccatgagtaa aactatattg aaccacattt
     1021 tccggaacca tcaaatcgcg cggagattcg atagttttaa ttattttttt atctctttcg
     1081 taaaattcca ccaaagcctg tgcaaatgtt gttttcccac ttccaggagc tccagaaacg
     1141 agaattcatt ttgcagatgt tttgaaccaa tctaaaactt cttgttctat tttatagtca
     1201 tccaaagata attttttaat tggtttgact accgtaattt ctagtccatc agaaagtggg
     1261 ggatatacga ttacaattct gtatggtcca agttgtatta tctttgaaag atttctatct
     1321 atctccaaaa tactatcatg cctagtttct acttctttaa aaatatcatc aattaatttt
     1381 tctacaaatt ttttgtccaa aactttgcta ccaagttctg gttttcgtcc accaggacct
     1441 ccattttttt tcaaaagagg gttgttttct ttgatatgaa tcgacatagt gttttcagca
     1501 aaatattggt ctaaaacact tccacaacct tttactattt gcatgtttta atcaaaaaaa
     1561 ataaaataat atttataaat aaataaactt ggatatttgt ttttgaaaat aaatcaccat
     1621 tacaaacaat acattataaa caatgtgtta tcgcaagtat cactataaac acaataattt
     1681 gtccaatttt ttgtttaaaa aaatcaacac ctctatatat tgatttttct ttatttttca
     1741 ataaataatg attattcaga aataaaagta cagtttttac ctacaaaaat tgatatgcat
     1801 tttcatataa ttggtataaa agaatacaaa aataataaat ataatatcaa cacatagttc
     1861 caaaataaac aaaaataaac attttattga ttattttata attaatctta tcctgttcta
     1921 tgctttaact aaataaaaga taatttatca ttttaaatta aaaaggtgac aatatttcta
     1981 cttatcttat caataatatc ttttgcttta tatttttata taatatgatt ttggtatctg
     2041 tgacttatac caattttact ttttcttctt ttttttatat acaacattag ttgactttcc
     2101 ctcaaataca aaaccaatag catattacaa aattatgctt tattttttgt acgaatcctt
     2161 atacagtttg gattattttt attatttaag ttttttgaca taaacaccat atgatccatt
     2221 ttcgccatca tgtctataaa tatcttgttt tggcttgggt cttatatctt taaatatgag
     2281 gattgaaaaa aaatagctca gtttgggtac tatattttca ttatattttt gttactttat
     2341 agtttatgaa attatgagat aacgataagc ctaaatttgt tgttttatgt acgaacattg
     2401 acccttgcta tagtaggttt tatagttttt gtgttgtcat tattttatca ggtggagaag
     2461 tatttagaat accaattttt tattttgatg ctatgaagtt tttgaatgat actctttaaa
     2521 caaataaaca atatttatat ttttctaagc atttttgttt ttattttgag tgtcctttat
     2581 atttatatct ttcgtatact ttccaataaa ccaccaacgg aagatcaaat caaagagatt
     2641 tccgttagac gcatattagc ttgagaaaga gtgttaaaag aaataaataa aaatagagat
     2701 ttttcaaata aaatgtattc ttttgttgca gggtttccaa gttttgtaaa atatggatta
     2761 gaattcgcaa atacattgat aattttaatt ttaatatatt tatattttca aaatgcttta
     2821 agtctaacat gaaatataga acaaatgttt tatcgattgg taacagcttg atttatagca
     2881 aatgtatatt tgttaaaaag attaaactac acctccatat tgcaaagact gcttactttt
     2941 ttggtaataa attttgctat atatattagt ttattttccg cattttcatg agatattgga
     3001 tctatagtat ttttaggaat aatacgaaat atactgtcaa caatgatggt ttttcatgtt
     3061 cacagaacaa aggtctgaga atatcttagg aaaatggatt atttgttttg gatatttacc
     3121 actatgttag ccttggtagt aaatatagtt ttgttattcc atactgattt ggctggaggt
     3181 ttattgttcc ctataatact tttatatgtt tgaatacagt gaatatcatt atactattcc
     3241 ataaggtata ttaacaaaat taaagaagtc acagaagagg atgagttgta aagttttagc
     3301 cccctttact tgttacggag aactccgtaa ataagggggg taggggggat tttgtgataa
     3361 atgagggttt ttatttagtc cccttgttta caaagaattc cgtaaatgag tgagttaagg
     3421 gagttaaaaa ccattcatta gtctaaaatc ccccttcgtc ccccttactc tccgatagat
     3481 ctcgaacaaa atgagatccc gatatatcgg gtcactttgt tcgaaggggg atttgaccat
     3541 caatcttcaa ctttcaactt tacaaacttt acaaactttc aacttgtaac cctcaacttg
     3601 caactttaaa ccaaaaaaag tatctcaaaa tttgcaaaaa atatttttat gaatataatt
     3661 tccaaggatt tagtttttta gaataaaaaa tgaaaacatt aataaaaatt ttgagcataa
     3721 ctttattttt actagcaatt ttttgaatat ctttcgcaca acacgggaaa tggggaaact
     3781 ggtgatcaga tccgatgcaa atttttaata cattcgtaga tagtggtagt aatgaatgaa
     3841 attataatat acatgaaact gctctagatt gagtgacaga tcaacaatga tgatatgcaa
     3901 ggtcttttaa aattacaaac acattagatt atattaggat ctacatggac ccatatcttc
     3961 aacgaacaat atatgctggt cttgttttag ctgttgttgc attgatatat ctttgatttt
     4021 tgttggtaac aggctgactt cacaaagaat gagatcgaag caaaataaaa tcaaaagtga
     4081 tgtatatagt tgttggggta tttttactta gttgatttta ttttatcatc aaagtaatgg
     4141 tggcgctcgt tacatcatta tttggatgat caacatgaga ttctggttat taaacgaaaa
     4201 acattttata taatataata tttaacaatg aaattattaa aatacttttt attcataata
     4261 tgagttcttt gatttttgtt ggttcaaaat aaggtgggtg cggctgtttg aattccccca
     4321 tcttttagga ataattttac agactatctt acgagttcgg aaccagacaa ggaatgaaga
     4381 gtagaaaatg ttattgattt atgaattgat actaataaat ctctgaaaca aaatataaaa
     4441 tgtctatttt atccaaatgc tagtcccgtt gtatgatgtg aaactacggt tgctggttga
     4501 aaaatatggg atgtggctag atatatttga tttgcaatat tagtaatctt tttggttgta
     4561 gttgggatca atttacttct acaatgatga aacccagaaa aagcaagatc atcattaaaa
     4621 tctttgatat acatactata tggttctgtt ttgttttttg gagctacatg gatactttga
     4681 agctctcttt gaattgaaaa tgttaagtga actgaatgat tagtggacgc tctacagtga
     4741 tgaccagatt ctttgttttt caaaattatt tcaaccttaa aggctttagc attttttgtt
     4801 gctatagtta tggttgttgt ttatggtttt aaaatgatgt cagtttccga tcaagcaacc
     4861 aaaacaaaaa cggctataaa atgaatcata aatgtaatta tagcattagt aattataaaa
     4921 gtaatagatt atgtttatta tattgcacaa ctaccaaatt ttacaacaca agcaacagag
     4981 tttattatag aggttgcaaa aatacttgga tttataatat gagctgtttt ggtgcttttg
     5041 gtattttact ccggtttttt gtttcttaca gatcaatgaa aatctgaaaa tatgaaaaaa
     5101 gcaaaaaata ttattgtttg agttatttta ggtgctatag taatatttat gttgttactg
     5161 ataatgtatc aactttttgc agagtttgca tagaatttta taaaaatagt tttttattct
     5221 caataaaacc caatgaaagt tatttgttca atctgatgag gagaaatatg aggatttttc
     5281 aatggcaaaa aaagaccaat agaaacaaaa gtaattgatc acgagttaat aactattagt
     5341 tgaaaaaaat ctcccaaagt gcttttcatc cctacagcaa gttgagacaa caagagttat
     5401 atttcaaatt ttactaatta ctatacctcg cttggatgtg atgtaaatac acttttgctt
     5461 cacaataaac ttattgatcc aataacattg aagaaaaaag ttttttcggc agacattatt
     5521 tatgtgtgag gtggaaatac actcaaaatg cttaatttgt ggaagaaagt ctgactcatt
     5581 tctcttttac aacaagcaca taaaaagtga atcatttttg ctggaattag tgcaggagga
     5641 atctgttggt ttcacggggg gatgtccgat tccagaaaat ttagtgacaa agatactgga
     5701 tttattattg taaaaggttt ggattttgtt cctctgtatt tttgccccca ttttgataga
     5761 gatgtcaaaa gaaaggaaat tttacccgaa tttatcaaaa ggacgaacaa aaaacgaatt
     5821 gttttagagg acggaactgc attccttgtc aggtgaaaaa ggtggagaat cgttaagtca
     5881 aaaccagaag cccgcgcttg tcttggtttt catcataaag atgaggtcat ctatcaagca
     5941 ttagaagtta cagactttca atctctttcc aaactttctc gtagctatcc cttgtttata
     6001 tagcctataa atttttattc tttcctctgg atttaaatat cagtttcccc cctttgcatt
     6061 actaatattg aataagattt aaattagcat ttctatctta tccattagtg gtgctttttg
     6121 aattataatt aacttcctct tgatttagat ggcatctttt cttattatcc atttgcatca
     6181 ctaatattct ttaagattta aattcccatt tattatctta tctattagtg gtgctttttg
     6241 aattataatt aatatcacaa tattccctct tgaaaaaaga tgtgttttgt atattttcaa
     6301 tatgttttat attatctatt aattaatttt atgtacaaaa atatgctcct ttcttgagaa
     6361 ttatcaaaac atgtttgaga aattgtagaa attcgtggat ggcttcatac tgttcgtgtg
     6421 gtatcagcaa atttagcttt tttaatacta agagatagag acggacttgc tcaagtggtt
     6481 tttgaagatg caaatgaaat taaaaaactt gagtgatgtc ttttatgaac tatactttct
     6541 gcaaaaggaa ctgttgcaga aatgccaaaa gggaagttcc gttttgaact tcaaaaggcc
     6601 tcagtaaatg ttatcagaaa agtggtttac ccgagtccaa tagatatttc caaagatgaa
     6661 attcatgctg actgagagac cttattagaa aataaagtag tagctttgag acatcctaaa
     6721 caaatgaaaa tattcaaact tgcagccatc atagaaaaaa atatgagagc attttttgag
     6781 cagaattgat ttacacaaat aaattcacca aaaattatcg gattcccaac tgaatgatga
     6841 gcagaggttt ttgaaattga ttattttggt cgtaaggctt atttagctca atctcctcaa
     6901 ttttacaaac aaatgatggt accagttttt gaaagagttt acgaaattgg aagagcttat
     6961 agagctgaaa aaagtaatac gagcaggcat ctttctgaaa ttttgatgct tgatatggag
     7021 atggggttta ttgatagttt tgacgacatt attgaaatga cagaaaaatt cgcaaatttt
     7081 gtggtaaaac aaacatggac tgaagcagaa catttgctca cagcacttgg agctacaaaa
     7141 ccacttcttg tagatcaatt tcctcgtatt agtgttgatg acttacacga actaatgctc
     7201 aaagaaactg gtgaagatta tcgtaaagaa aaagatctag caccatcaga agaaaaattt
     7261 atttgtgaat attcggcaaa acactggaat tcggattttg taattgtaga ttgattccct
     7321 tggtctgatg ctaagttcta tcatcaccaa aataaagaca atcctaatat agcagatcgt
     7381 gcagaccttc tctttagagg ggtagaaata gtaacattaa ctcagcgcga agtaaattat
     7441 caaaaactag tagatcaaat taaagcacaa gggcaaaatc cagaacatcc tggcttaaag
     7501 cattacctag attcctttaa gtatgggatg cctgatgaat gaggttttgg acttggaatt
     7561 actagattta ttcaaaaact tattggatta gaaaacatca aagaagc
//
LOCUS       DAOSID010000025         7515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_9010_length_7515_cov_12.357507, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000025 DAOSID010000000
VERSION     DAOSID010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 7515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7515
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9010_length_7515_cov_12.357507"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..475
                     /locus_tag="PK674_01870"
     CDS             131..475
                     /locus_tag="PK674_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15312.1"
                     /translation="MAELIGSGINLSSINTWKDFEKQEVSKVLSKGVKKTSKGTTLAL
                     LGLMSLGVGFSSCCGSKTVEQEINDLVADKQEQVDKLTREELKLQKKLDKVKADKKFA
                     EEELKDAKSRQK"
     gene            560..2299
                     /locus_tag="PK674_01875"
     CDS             560..2299
                     /locus_tag="PK674_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="HD domain-containing protein"
                     /protein_id="HOG15313.1"
                     /translation="MLNFDGFEKLDIHQRYLQNKSKTENLFLVGGTIRDLILGIEKNP
                     KDIDFTMAGFPMDIYDKIDKRKVSHFITEKFGTITLIPKNQEVKYELTPFRLENQYSD
                     NRHPEKIQRQDDLILDSNRRDFTVNSLYYFSTNIKNKILLSSKLGKKNISNIINFQDS
                     LEKHGFLFYQDQNLLIVQNHNYIQNLFPKGKFSLDFAVYLLDTLKEIFVKKQKSEENI
                     IRILIDPHGAIQDLINRKIKCVGEADNRFREDALRTIRALRFVSVLNQKLRNKKGSGK
                     MLLFDIETNTWNSLKKHVHLVHNVAKERIKDEIKKVFSVGDPFVFISLLDEIKLLEHL
                     FPGLYKTKHVEQPVRYHPFDIYVHTMLSLYELQKINSDYLVRLAMLYHDVGKVGQYEA
                     YQTGLDRDEIRKILSGPLNHRSSGPEIAKEDFSKLGFSKNEINDIMRYIANHHVPGEI
                     LDAKNYNRVKKIRKLYSEAGFQKVDNLLDITIADRIGQYNPMQNNSDITDIEYLRKIL
                     KKLQKNEGQFTARDLKIDGEIIMDFFQIQPGQKVGELLRYALDWVIGDVKNRNNKDEI
                     LKYLKGVYKVKGL"
     gene            complement(2381..3508)
                     /locus_tag="PK674_01880"
     CDS             complement(2381..3508)
                     /locus_tag="PK674_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15314.1"
                     /translation="MKKSKILLSLLGLLAFLAIPVQASSIFSGSEQIVNIMEPVHSNM
                     YVATSNLIVSNPIGGDLIAFGESISIHSNISKDFMGFGSNIHINGKVADDVRIAGQSI
                     FINQDIGGDFIAFGASVTVAPGVKIGGDFIVYGAAVNMDGIVNGNAYIGSAGLSMNGQ
                     IKGDAKLTFDEIKLGDNAKINGSLNYKAPQTNEQLEGISKGEVIYKSSVKTDSKKFLW
                     GFIKWIVFYKWAYISLFALILVFVFKRILKEVSDTLKKKPRQSLFTGFLCYVVPPFAI
                     LTLIITFLGIPLAGILALLYIALLIMGVIVSSAIFSSWFIDNFRGGIENTTWWKILLT
                     VIVVSFFFTIINSIDIIAVFFAIGALALYKIKSTKKTLKSL"
     gene            3842..6361
                     /locus_tag="PK674_01885"
     CDS             3842..6361
                     /locus_tag="PK674_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HOG15315.1"
                     /translation="MPTVLARIIGIILFVLIGVSGYVLLFLQLEKRHRFKQTKHIRFL
                     QVRIQKKTAAKSVDIEATDHIQQMKNNIEIMNQVYKNFYSVYRHLRSLFETDFSKSFF
                     GDLKYRLFGGDYISMELFVEKEQIKYIVGVPDGQLSTVRKMIAAFYPESFIEDIEQPK
                     LLEAGKYMAGGEFNFVENSVYPIKTYEAFEADPMESILSSYSNVGKDEKMVLQILVEP
                     LPSKRLKKMRKKADKIKTGKDFGFFKKIFLKLRKGFSNDDTKHEKNQEEKSKHNFSQQ
                     QLGDFDKKTDDEIFRTKIRTFATSTEKNRPAKMIDEMERLFNQYNYIGLNRLKFWKIK
                     NLRSFAKDFVLRNFYTNSSFYYQLKSFFRPNILNIKELSSIVHFPHARFNLNPRIAWQ
                     KAKIVPAPENMPSDGMHLGRNEYGGVKRDVILSDKDRFRHVYIVGQTGTGKSTMILTQ
                     AKEDMLRGNGFCVIDPHGDLVDTLMKHFPKERIDDLIYFDLSNTEYPIAFNPLDGAHT
                     DDERDVVTNDMVEMFVDMYGPEIFGPRIQDYFRHACFLLMEQPDGGTLVDIMRLFTDD
                     AYAESKIRNLTNPVIASWWNKTYKKMGDREKAEIIPFFQAKFGPFTTGTYIRNVVGQP
                     KSAFNFFDAMQEGKVIMCKLAKGLTGEVNSQLIGRMVAMQIKLSALKRAALPEEERKP
                     YFLYVDEFQNYVSQSFESILSEARKYRLGLTVAHQYIDQLKKEGLGGSLDLSKTIFGN
                     IGTIFALKVGAPDAEFLENEFSPEFTKMDLQSSEMFKGIMKMSINGSQSRPFSLTTRT
                     IYGDPPLNTPEKIEIMKQISSLKRGTKRELVDKEIYFRVGV"
     gene            6863..>7515
                     /locus_tag="PK674_01890"
     CDS             6863..>7515
                     /locus_tag="PK674_01890"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="TPM domain-containing protein"
                     /protein_id="HOG15316.1"
                     /translation="MKKIGFFILSIFCLFGISFANTIDPMNLPKFEYFINDYSQVLNQ
                     EQTQELNQYAENIESNLGYQVVSVLFPHRQGNELFDIALKAFNENGIGDKQRNDGLLL
                     AIATEEKKIRIMVGYGLEGKIPDLLASQIIEEKIRPEVNQGNIYQGIKNFYEVIDGKQ
                     SLPEISQEEGSDWIYTVIMWIIIFALIVLYIIFDGGLGFGGGSSGGGGGFSGGGGSSG
                     "
BASE COUNT         2767 a          916 c         1323 g         2509 t
ORIGIN      
        1 gaaaaatatc aagcaaataa gtataaaaaa cgaacttaac tctgcaatag cacaattatt
       61 tgacaaattt agaaaaataa ttataattgt tgtgtcaaaa atttaatttt atatcttaat
      121 aaaaacaaaa atggctgaat taataggtag tgggatcaat ctttcttcaa ttaatacttg
      181 gaaagacttt gaaaaacaag aagtttctaa agttctatct aaaggagtaa aaaaaactag
      241 taagggaaca actcttgcat tgcttggttt gatgagttta tgagtttgat tttcgtcttg
      301 ttgtggatca aaaactgttg agcaggaaat taatgatctg gttgcggata agcaagaaca
      361 ggttgataaa ttaactagag aagaacttaa attacaaaaa aaactggata aggttaaggc
      421 agataagaag tttgctgagg aggaacttaa agatgcaaag agtagacaaa aatagaaaaa
      481 tatagtttta tttcttgata aaaaataacg attctctatt atgagtttgt tatttttaaa
      541 aaaattaaaa aaaaatgcta tgttaaattt tgattgattt gagaaactag atatacatca
      601 gagatatctt cagaacaaat ctaaaactga aaatcttttt ttagttggag ggacgattag
      661 ggatttgatt ttgggaattg agaaaaatcc taaggatatt gattttacga tggctggctt
      721 tccgatggat atatatgata aaattgataa aagaaaagtt tctcatttta ttacagaaaa
      781 gtttggaact attacattga ttccaaaaaa tcaggaagta aaatatgaat tgaccccatt
      841 tcgtttggaa aatcaatatt ctgacaatcg tcatccagaa aagattcaac gacaagatga
      901 tttgatttta gattctaata ggagggattt tactgtcaat tctctatatt atttttcgac
      961 aaatataaag aataaaattt tattatcttc aaagttgggg aaaaaaaata tatcaaatat
     1021 tatcaatttt caagacagtt tggaaaaaca ttgattttta ttttatcaag atcaaaatct
     1081 tttaattgtt caaaatcata attatattca aaatttattt ccaaaatgaa aatttagttt
     1141 ggattttgct gtttatttgt tggatactct caaggaaatt tttgttaaaa aacaaaaatc
     1201 tgaggaaaat attattcgta ttttaataga tccacatgga gctatccagg acttaataaa
     1261 caggaaaatt aaatgcgttg gagaagcaga caatagattt agggaagatg ctttgagaac
     1321 tatccgtgct ttgagatttg tttctgtgtt aaaccaaaaa ttaaggaata aaaagtgaag
     1381 ttgaaaaatg ctactttttg atatagaaac taatacttgg aattctttaa aaaaacatgt
     1441 acatttggtg cataatgttg caaaagaaag aataaaagat gaaataaaaa aggtcttttc
     1501 tgtttgagat cctttcgttt ttatttcgct tttggatgaa ataaaattgc tagaacatct
     1561 tttcccatga ttatataaaa caaaacatgt ggaacagccc gtgagatacc atccttttga
     1621 tatttatgtt catactatgt tgagtttgta tgaattgcaa aaaataaatt ctgattattt
     1681 ggttcgtttg gctatgttgt atcatgatgt tggtaaggtt ggacaatatg aagcttatca
     1741 aacaggtttg gatcgtgatg aaattagaaa aatattgtct tgaccattaa atcatcgtag
     1801 ttcttgacca gaaatagcaa aggaagattt ttctaaatta tgattttcaa aaaatgaaat
     1861 aaatgatatt atgcgatata ttgccaacca tcatgttcca tgagaaattt tggatgcaaa
     1921 aaattataat agggtcaaaa aaattagaaa actttatagt gaagcatgat ttcaaaaagt
     1981 agacaatttg ttggatatta cgatagctga tagaattgga cagtataatc ccatgcaaaa
     2041 taatagtgat attacagata tagaatattt gagaaaaata ttgaagaaac tacaaaaaaa
     2101 tgaatgacaa tttacggcaa gggatttgaa aatagattga gaaattatta tggatttttt
     2161 tcaaatccaa ccatgacaaa aggtttgaga gttattgagg tatgctttgg attgggtaat
     2221 aggtgatgta aaaaacagaa acaataagga tgaaattttg aagtacttga agtgagttta
     2281 taaagttaaa ggcttataaa aacctaccac ccgggtgctg tttttgaccc cggggtcaat
     2341 tttttcaccc gggtggtagg ggaaaaggag ttgatttact ctaaagggat tttagagttt
     2401 tttttgtgga ttttattttg tacaaagcca atgctcctat tgcaaagaat accgcaataa
     2461 tatcaatact attgataatg gtaaaaaaga atgaaactac gatgactgtc aaaagtattt
     2521 tccaccaagt agtattctcg ataccacccc ggaagttatc tataaaccaa gatgagaaga
     2581 tagcactgct aacaataact cacattatca atagagcgat gtataaaaga gccaaaattc
     2641 atgcgagtgg aatacctaaa aaggttataa taagtgtaag aattgcaaat ggaggcacaa
     2701 catagcatag aaatccagta aataaacttt gccgtggttt cttttttaaa gtatcagaaa
     2761 cttctttaag aatcctttta aatacaaata ctaaaataag tgcaaataaa gatatgtatg
     2821 cccatttgta aaatacgatc cattttataa atccccaaag aaactttttg gaatcagttt
     2881 ttacggatga tttataaata acctctccct ttgatattcc ttctagttgt tcattagtct
     2941 gtggggcctt ataatttaaa gaaccgttta tttttgcgtt atctccaagt tttatctcat
     3001 caaaagtgag ttttgcatct cctttaattt gtcaattcat tgagagtcat gcagagccaa
     3061 tgtatgcgtt accgttaacg ataccatcca tatttacagc tgctcagtaa acaataaaat
     3121 ctcatcaaat tttcacccca ggagcaacag taacagaagc tccaaatgca ataaagtctc
     3181 atcaaatatc ttgatttata aaaatacttt gtcatgctat tctaacatca tctgcaactt
     3241 taccattaat atggatattt gatccaaatc acataaaatc tttgctaata ttggaatgta
     3301 ttgaaataga ttcaccaaaa gcgattaaat caccaccaat aggattggaa actatcaaat
     3361 tacttgtggc tacatacatg tttgagtgta cgggttccat tatattaact atttgctctg
     3421 atcaggaaaa aatggaacta gcttgaacag ggatggctaa aaaagccaat agtcctaaaa
     3481 gactgagaag aattttgctt tttttcatgt tgaatagaaa aatataaaaa attttaccgt
     3541 ataataagaa aaaaaaagtt tttttcaata gtagggcagg gtaataagtt agaaagttta
     3601 taaagttgca ttctcctacc acctgccccg cagtactgcg gggcgggtgg taggaatgta
     3661 acttttaact tgtaactttc aactttataa aatttcaact atgttatata tcttttttgt
     3721 tattttggtt gttaatttga tgtttaaact caaaatcttt tcctatttta tgtgaaaatc
     3781 attataattt tgtataagga taatgtttat cttttataag ttttattttt gtaatatgta
     3841 tatgccaact gtcttagcac gaattatagg aataatatta tttgtattga tatgagtaag
     3901 ttgatatgtc ttattatttt tgcaattaga aaaaaggcat agatttaagc aaactaaaca
     3961 tattagattt ttacaagtta gaattcagaa aaaaacggca gcaaaaagtg ttgatataga
     4021 agctacagac catattcaac aaatgaaaaa caatatcgaa ataatgaatc aagtttacaa
     4081 aaatttttat tctgtttata ggcatttacg ctctttattt gagacagatt tttcaaaaag
     4141 tttttttgga gatcttaaat atagattgtt tggaggagat tatattagta tggaattgtt
     4201 tgtagagaaa gaacaaataa aatatattgt ttgagtgcct gatggtcagt tatctaccgt
     4261 aagaaaaatg atagcagctt tttatccgga atcgtttata gaagatatag aacaacctaa
     4321 attgctggaa gctggtaaat atatggctgg tggtgagttt aattttgttg aaaatagtgt
     4381 atatcctata aaaacttatg aggcttttga agctgatccg atggagagta ttctttcttc
     4441 atatagtaat gtttgaaaag atgaaaaaat ggttttacaa atattagtag aaccacttcc
     4501 atccaagcga ttgaaaaaaa tgagaaaaaa agctgataaa atcaaaacat gaaaggattt
     4561 tggttttttc aaaaagatat ttttgaagtt acgaaaatga ttttcaaatg atgatacaaa
     4621 acatgaaaaa aatcaagaag aaaaaagcaa gcataatttt tctcaacaac aactttgaga
     4681 ttttgacaaa aaaactgatg atgagatatt taggactaag atcagaactt ttgctacttc
     4741 tacagaaaaa aacagaccag ctaaaatgat tgatgaaatg gaaagattgt ttaatcaata
     4801 taattatatt ggtttaaata gattgaaatt ttggaaaata aaaaatttga gatcttttgc
     4861 caaagatttt gtgttgagaa atttttatac aaattcatcg ttttattatc aactaaaatc
     4921 cttttttaga ccaaatatat taaacataaa agaactttct tctatcgttc actttcctca
     4981 tgcgagattt aatcttaatc ctcgtattgc ttggcaaaaa gcaaaaattg tacctgcacc
     5041 agaaaatatg ccatctgatg gaatgcattt gtgacgaaat gaatatgggg gagtaaaaag
     5101 agatgtgata ctttcagata aagacagatt taggcatgtt tatatagtat gacagacagg
     5161 tacaggtaaa tctactatga ttttgactca agcaaaagaa gatatgttga gatgaaattg
     5221 attttgtgtg attgatccgc acggagattt ggtagatact ttgatgaaac atttcccgaa
     5281 agaaagaatc gatgatttga tatattttga tttatctaat actgaatatc caatagcatt
     5341 caatcctttg gattgagctc atacagatga tgaaagagat gttgtgacaa atgatatggt
     5401 agaaatgttc gttgatatgt attgacctga aatatttggt cctcgtattc aagactattt
     5461 ccgtcatgct tgttttttac ttatggaaca acctgatggg ggtactttgg ttgatataat
     5521 gaggttgttt acggatgatg cttatgcaga atcaaaaatt agaaatttaa caaatcctgt
     5581 aattgcatct tggtggaata aaacttacaa aaaaatgtga gatagagaaa aggcagaaat
     5641 tattccattt tttcaggcaa aattttgacc ttttacaact tgaacatata tcagaaatgt
     5701 tgtttgacag ccgaaatctg catttaattt ttttgatgct atgcaagagt gaaaggtgat
     5761 aatgtgtaaa ttggcaaaag gtttaacttg agaagttaat agtcaactta ttggtagaat
     5821 ggtggctatg cagattaagc tttctgcttt aaaaagagcg gctctaccag aagaagaaag
     5881 aaaaccatat tttctatatg tggacgaatt tcaaaattat gtttcacaat cttttgaaag
     5941 tattttatca gaagccagaa aatatagatt atgattaact gtagcacacc aatatataga
     6001 tcaattgaaa aaagagtgat tgtgatgatc attagatctt tcaaaaacaa tatttggaaa
     6061 catttgaaca atttttgcat tgaaggtctg agctccagat gcagaatttt tggaaaatga
     6121 gtttagccca gagtttacaa aaatggattt acaaagttcg gaaatgttta aatgaattat
     6181 gaaaatgagt ataaattgat cacaatctag gcctttttcg ttaacaacta gaacaattta
     6241 tggagatcct ccattgaata ctccagaaaa aatagaaatt atgaaacaaa tttcttcttt
     6301 gaaacgatga acaaaaagag aattggtaga taaggaaatc tactttagag tgggagtgta
     6361 gagaggtgtt agtccttagt cgctagtcga tagtcgttag tcattagtcg atagtcaata
     6421 gtcgttagtc attagtcgat agtcgttagt caatagtcga tagtcaatag tcgttagtca
     6481 atagtcgata gtcgttagtc aatagtcgat agtcaatagt cattagtcga tagtcaatag
     6541 tcaatagtcg atagtcgtta gtcaatagtc gatagtcgtt agtcaatagt cgatagtcaa
     6601 tagtcattag tcgatagtcg ttagtcgcta gtcgatagtc agtagtcgat agttttttcc
     6661 tacttgtggc ttaaatttct gtaaaatata gccactctct tcaaattcgc acctacaaaa
     6721 tagatttgaa gagaagctca aaatttgtta tagcgaataa gttgtcattg ttagttggta
     6781 aatattactg ttttttttag tttttattga tattatttta tctattcgta taaaaatatt
     6841 gctttacaat attcaataaa tcatgaaaaa aatctgattt tttattttga gcatattctg
     6901 tctttttgga atttcttttg caaatacgat tgatccaatg aatttgccaa aatttgagta
     6961 ttttatcaac gattattctc aagttctcaa tcaagagcaa actcaagagc ttaatcagta
     7021 tgcagaaaat attgaatcta atttgtgata tcaggtagta agtgttcttt tcccacatag
     7081 acaaggaaat gaactttttg atattgcttt aaaagctttt aatgaaaatg gaattggcga
     7141 taaacaaaga aatgatggac ttttgcttgc cattgctaca gaagaaaaga aaattagaat
     7201 catggtatga tatggattag agggaaagat acctgatctg ttggcaagtc aaattattga
     7261 agaaaagatt agaccagaag ttaaccaatg aaatatttac caaggaataa agaattttta
     7321 tgaagttata gatgggaaac agagtcttcc agaaattagt caagaagaat gatctgactg
     7381 gatatatact gtaattatgt ggattataat ttttgctttg attgtcttat atataatttt
     7441 tgattgaggg cttggtttct gaggatgaag ttcgggcgga ggatgatgat tctctggtgg
     7501 aggatgaagt tcggg
//
LOCUS       DAOSID010000026         7484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_9052_length_7484_cov_9.406380, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000026 DAOSID010000000
VERSION     DAOSID010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9052_length_7484_cov_9.406380"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..1320)
                     /locus_tag="PK674_01895"
     CDS             complement(265..1320)
                     /locus_tag="PK674_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15317.1"
                     /translation="MEYYKLTLDDFKRVFEFGTNYYIDPSKNTTGRTTGEPRGLGAIL
                     DAFTLGKLTEIGIEKIFTEFNKNKAYILDFDIKSNSQVKDEPDIVKIKEQNKFREPNV
                     FVEIKNTSESDRWIGLTEEQFSTIKRSAGNKEIYMIYASLRSETRENNPKTTDLTGMF
                     LKEIEDKNKSEIFQKFANLNAECKIEFIISSKDLDKFSYPFERGMNMYETNLFNEKKS
                     TSFYSKNSIRKDVLKIDEYKNFDNTMELSIETNLYPEKDEISKFKVNGSFNLIQKKKK
                     TYIECISDVYIENDIFGYFNLKKDKFYSFNLATVGRDPKLKRNNLFIAKNKVYQLLES
                     GKIRKPEIIVKEIVKKI"
     gene            complement(1342..2298)
                     /locus_tag="PK674_01900"
     CDS             complement(1342..2298)
                     /locus_tag="PK674_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOG15318.1"
                     /translation="MKFIDLFAGIGGIRLAFENAGFQCVFSNDFDNNCKITFDHNFAD
                     FFDIKNQMYLGDITKIPIKKIPDCDILTGGFPCQPFSIAGYRQGFDDEKGRGNVFFDI
                     IRILKDKKPKAFLLENVKNLKTHDNHNTIKIIYQELQKLGYKVADKVLNTMEYGNLPQ
                     NRERIYIVGFLDEESFNKFQFPTKIPLTKTIQDCLETSEVDIKYYYNNKPLYERIKND
                     IVKKDTIYQWRRKYVRENKNNVCPTLTANMGTGGHNVPIILDDIGIRKLTPRECANFQ
                     GFPKNYKLPNIADSELYKQFGNSVSIPVVQRIAEKMKLALSK"
     gene            complement(2469..2744)
                     /locus_tag="PK674_01905"
     CDS             complement(2469..2744)
                     /locus_tag="PK674_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15319.1"
                     /translation="MQRGISLVGGTAGPLIHSSNTNNRGALASLSNINGIKYNQKKSE
                     VKGNVKAEGLAEISKKEPKRNDLLVFVLYHISRSFDNTKKNDTNTRR"
     gene            complement(3101..3295)
                     /locus_tag="PK674_01910"
     CDS             complement(3101..3295)
                     /locus_tag="PK674_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15320.1"
                     /translation="MGKRINLAQISPKFSALQQALIEAKSPEEKIEIMLTLIPKEERA
                     TKREELLKSLEELRKKNKNI"
     gene            complement(3549..3788)
                     /locus_tag="PK674_01915"
     CDS             complement(3549..3788)
                     /locus_tag="PK674_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15321.1"
                     /translation="MLSENLSNRKRLATDSRALGAPSLQPTLSRYSVKISSENANLFK
                     RIDNKDNLYLDMVIVIPKNLKDKAIELGKNIIKKQ"
     gene            complement(4132..5049)
                     /locus_tag="PK674_01920"
     CDS             complement(4132..5049)
                     /locus_tag="PK674_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="phosphotransferase"
                     /protein_id="HOG15322.1"
                     /translation="MGKQIEKQILIKILLKKGHIKGNSNFIEVHESCGNQVFCTDEIF
                     IKVSPNVNNLKTEYNILFLLDNKHFPKPLFYEKISDNSGTFYVLGISKIKGKTLEHYR
                     EKMNGEEKNQLILNLISCMKYINSFQLSNGNQSLYDNRTNIVNQGKYRTNPNIDPKSL
                     QHLIDEVNVFLKEIKNEKQFLIHNDIWYKNILLDNNIFSGLIDFETSYFAPIRFEYYS
                     LLLTNYYVLTTECGDSGNCEKEFCKMLSDSIESHYSDLLKNSSKKEFICFCIISYLKK
                     LGQYEEERYKHEEVIGFMNDIEKFSLMIF"
     gene            complement(5072..5824)
                     /locus_tag="PK674_01925"
     CDS             complement(5072..5824)
                     /locus_tag="PK674_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOG15323.1"
                     /translation="MKQIILCDNLNIEAVSPKAKNNGFGIEVQLFSKPTKCEDEAQIN
                     FHKEKIKGINPISLHAPFGDLCPGSSDPMVREVARNRFELGYKTANQLGAKSIIHHIG
                     RVPKAGTAQKRAEHCVDFWKDFLKGKSENINFYIENLFDHNPEVLSLVIDGINKNNVK
                     VCLDIGHAHCYSKIPVLDWIKILKDKIGYVHIHDNNGAEDEHLGIGKGNIPMFEVFSA
                     LNKYAPNAIRALECCIDHMDESITWLKNNDFL"
     gene            complement(5930..6220)
                     /locus_tag="PK674_01930"
     CDS             complement(5930..6220)
                     /locus_tag="PK674_01930"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOG15324.1"
                     /translation="MKFSDGIGYKKRPVVILNNNRGDFLICAISTQLQQGGQFDIYLN
                     CDSENNLRKDSVILLFKISTISSESITTKMGKLSKNNWILLKNNMQKFVNSW"
     gene            complement(6246..7043)
                     /locus_tag="PK674_01935"
     CDS             complement(6246..7043)
                     /locus_tag="PK674_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF4065 domain-containing protein"
                     /protein_id="HOG15325.1"
                     /translation="MDYSIIKKLRKKNNWTQDQVAEMLGISRVTYNLLENGKTPRTPY
                     IKSLQDIFDISFSALQNNKKIKHKTLNDRQYKKIKNLILYILNKTSSLPNVGKTVIYK
                     ILYFCEFDRYELTGERLTGLDFIKLPKGPAPENFDFIINRMEKEESIISITAKYMGYI
                     QQRYIINEKVADNLLEGEQKTHVNKVIEKFKNMNASEISEYSHGDIPWKATPDMEIID
                     INLANHRLYPYSAKIREKKIEQDTNNFTNNPAFQFLLDEEDLYEDIV"
     gene            complement(7261..>7484)
                     /locus_tag="PK674_01940"
     CDS             complement(7261..>7484)
                     /locus_tag="PK674_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15326.1"
                     /translation="KISLSNNQKEIGNIRTSLINDFLAQDDKGNYQYEGYTDNMKQII
                     ISSKKPLQIGDFTETKITNGVVFKLFGEC"
BASE COUNT         2329 a         1043 c          946 g         3166 t
ORIGIN      
        1 tttcactatc cttaccacat tcttcatact gataaattgc atcagagatt atctggtctg
       61 tattttggtt tgttattatt tgagtttcat ttgtgttttc aattttattg aaacagccag
      121 tgattagaat actggctaat aatgatggaa ctataaatcg tgtttttttc atgatgattt
      181 atttaagagt taaatgtttt tttattttaa ttttgttcaa aaaggggttc ttttgtaatg
      241 gaatgtaata ttgaaaattg tttattatat ttttttaact atttctttta ctattatttc
      301 aggttttctt attttaccag actctaatag ttgataaact ttattttttg caataaaaag
      361 attgtttctt tttaattttg gatctctacc aacagtagct agattaaaac tgtaaaactt
      421 atctttttta agattaaaat atcagaagat gtcattttca atatatacat cagaaataca
      481 ttcaatatat gttttctttt tcttttgtat caagttaaaa cttccattta ctttgaattt
      541 tgaaatttca tctttctctg gatataaatt ggtttcaatt gataactcca ttgtgttgtc
      601 aaaattttta tattcatcaa tctttagaac atcttttctt atactgtttt ttgagtaaaa
      661 tgaagtgctc ttcttttcat taaacaaatt agtttcatac atattcatac ctctctcaaa
      721 tggatatgag aacttatcta aatcttttga tgatatgata aattctattt tgcattctgc
      781 attgagattt gcaaattttt gaaatatctc acttttgttt ttgtcttcaa tttctttcaa
      841 aaacattcct gttaaatcag ttgtttttgg attattttct cttgtttctg agcgaagtga
      901 agcatagatc atataaattt ctttgtttcc agcacttctt tttattgtgc taaattgctc
      961 ttctgtaagc ccaatccaac gatcactttc agaagtgttt ttaatttcaa caaaaacatt
     1021 cggttctcta aatttatttt gctctttgat ttttactata tctggttcat ctttcacttg
     1081 agaattactt tttatatcaa aatctaaaat ataggctttg tttttgttaa attcagtaaa
     1141 gattttttct atacctatct ctgtaagttt tcctaatgta aaagcatcaa gaatggcacc
     1201 gagtcatcta ggttctccag ttgttcttcc tgtggtgttt ttagaggggt caatataata
     1261 gtttgttccg aattcaaaga ctcttttgaa gtcatctaat gtgagtttat agtattccat
     1321 tcgaaaaaat taaaagtaaa attattttga aagtgctagt ttcattttct cagctattct
     1381 ttgaacgaca ggtatggaaa cagagttacc aaactgttta tatagttcag aatcagcgat
     1441 atttggtaat ttatagtttt ttggaaaacc ttgaaaattt gcacattctc ttggtgttag
     1501 ttttcttata cctatgtcat caagaattat tgggacatta tgtccccccg ttcccatatt
     1561 tgcggttaat gtaggacaaa cattattttt attctctctt acatattttc ttctccattg
     1621 gtaaatggta tcttttttaa caatgtcatt ttttattctt tcataaagag gtttattatt
     1681 ataataatat ttaatgtcga cctcacttgt ttccaaacag tcctgtatgg tttttgttaa
     1741 tggtattttt gtagggaatt gaaatttatt aaaactttct tcatctaaaa atcctacaat
     1801 ataaattctt tctctatttt gtggtaagtt tccatattcc atagtattta atactttatc
     1861 tgctacttta tatcctaatt tttggagttc ttgataaatt attttaatcg tattgtgatt
     1921 atcatgagtt tttaaatttt ttacattttc aagtaaaaat gcttttggtt ttttatcttt
     1981 tagaattcta ataatatcaa aaaaaacatt tcctctacct ttttcatcat caaaaccctg
     2041 tctatatccg gcaatagaaa atggttgaca aggaaaccct cctgtaagaa tgtcacaatc
     2101 tggaattttt tttataggaa tttttgtaat atcacctaaa tacatttgat tttttatatc
     2161 aaaaaaatct gcaaaattat ggtcaaaggt aattttacaa ttattatcaa aatcgttaga
     2221 aaaaacacat tgaaatcatg cattttcaaa tgctaatctt atgcctccaa tacctgcgaa
     2281 taaatctata aatttcataa gtttaaaaaa ctagataaag tcctcttata taaagtatac
     2341 taaaataatt gtgtttttca aataataaaa ctaacttaaa ctttgtttcg tttgttattt
     2401 gtttttttgc tgtaaaatac taacaaaaat tgaattacta aatttctcta tattatctat
     2461 aaatcaaatt acctccttgt gtttgtatcg ttcttcttcg tattgtcaaa gcttcttgag
     2521 atatgatata atacaaaaac aagtaaatca tttcttttgg gttctttttt acttatctca
     2581 gccaatcatt ctgctttcac attccctttg acttctgatt ttttttgatt atacttgatt
     2641 cagttgatgt tggaaaggct tgctaacgca cctctattat tagtattaga ggaatgaatg
     2701 aggggtcaag ccgtccctcc aactaatgag atacctcttt gcaatatgga tttgaggtat
     2761 ttttttgtat ttgaattatt ggggaacttg tcaaaaaaag taacagtact atatcaatta
     2821 aacccactcc cagccaacag acttagaaaa cataaaaaat ttttactgtt gtagatagat
     2881 ttagagtaaa aaacttaaat aattacacat atctaaacaa tacttgattt tgaattttat
     2941 cctgcatttt atgtgtttct gactataatt ttagtgtttg atggatagat aacgttttgg
     3001 atgtaatatc tagagcataa gtattagata ctatcttcac aaaaaaaaca cacaaagtgt
     3061 gtctttttta ttatggttta ttgatatatt aatgcaaatt ttagatgttt ttattttttt
     3121 tcctaagctc ttctagactc tttaataatt cttctctttt tgttgctctt tcttctttag
     3181 ggataagtgt aagcattatc tctatttttt cttctggaga ttttgcttca ataagtgctt
     3241 gttgtaaagc agaaaactta ggtgatatct gtgctagatt tattcttttc cccattgttt
     3301 ttaagaatta aaaaataaaa attaagcatt attttcagtt ttttttcctt attctattac
     3361 cttcaaaaat tcttataaga tttttcccct tttcctttca ggtcatatct ttgatgaaat
     3421 caaggttttt tacattacta ttcctttaaa aataaaatac aatatctttc agaacttttc
     3481 tctcatttac tagcacataa caccatctca gatggtattg atttcttcaa atatttcaag
     3541 attatatatt attgtttttt gattatattt ttacctaatt ctattgcttt atcttttaag
     3601 tttttaggta taacaattac catatctaaa tataaattat ctttattatc tattctctta
     3661 aaaagatttg cattttcaga agaaatcttt acactatatc ttgaaagggt tggttgcaac
     3721 gacggtgcac ctagtgcacg gctgtcagta gccaaccttt ttctattact caaattttca
     3781 ctcaacatat ttctatcaat ctcaccatcc agaatatcat caaagagctg ttttactttc
     3841 tttttattct tatttactcc tatgatccag tcttttactt ttttgaagaa atcttttatt
     3901 gattgtataa gtttagcact taatgatttc ctaaccttcc caaccgttgt ttctctgttt
     3961 gttaattggt ctaaatttct ttaaaatatc ttgcattatt tttatttatt tgtatacttt
     4021 tgttcagtgg tgtaccaatg ctccctcctg ctttatgcag ttggttgagc ggtacatcac
     4081 tttttttatt actcaaattt tcactcagca tattcctatc aatctcacca tctagaatat
     4141 catcaaacta aatttctcta tatcattcat aaatcaaatt acttcttcat gtttatatcg
     4201 ttcttcttcg tattgtcaaa gcttcttgag atatgatata atacaaaaac aaataaattc
     4261 tttctttgag ctgtttttta gtaaatctga ataatgactt tcaatagaat cggaaagcat
     4321 tttacaaaac tctttttcac aatttcaact atctccacat tcagtagtta aaacataata
     4381 atttgtcagt agtaaggaat aatattcaaa tctaatggga gcaaaatagc ttgtttcaaa
     4441 atcaattagt ccagaaaaaa tattattatc caaaagaata tttttatacc aaatatcatt
     4501 gtgaataaga aattgttttt cattttttat ttctttcaaa aaaacattaa cttcatcaat
     4561 taaatgttgt aatgatttcg gatcaatatt tggattagtt ctatattttc attgattgac
     4621 aatatttgtt cgattatcat aaaggctttg atttcaattt gaaagttgga aactgtttat
     4681 gtatttcatg catgaaatta gatttaatat aagttgattt ttttcttctc aattcatttt
     4741 ttctcgatag tgttccaatg ttttaccttt gatttttgaa attcataaaa cataaaatgt
     4801 accagaatta tcagaaattt tttcataaaa taatggtttt gggaaatgtt tattgtctaa
     4861 taaaaataaa atattatatt ctgtttttaa gttgttaaca tttgggctta ctttaatgaa
     4921 aatttcatca gtacaaaaaa cttgatttcc acaactttca tggacttcaa taaaatttga
     4981 attcccttta atatgtcatt ttttcaaaag aatttttatt aaaatttgtt tttcaatttg
     5041 ttttcacatt aattttttga ttaagatatg tttacaaaaa atcattattt ttgagccaag
     5101 ttattgattc atccatatga tcaatacaac actctaaagc tcgtattgca ttaggtgcat
     5161 atttgttcaa agctgaaaaa acttcaaaca ttggaatatt tcatttccca attcataaat
     5221 gttcatcctc tgctccattg ttatcatgaa tatgtacata accaattttg tcttttagta
     5281 ttttaatcca gtctagtact ggtatttttg aataacaatg agcatgtcat atatctaagc
     5341 aaacttttac attatttttg ttgataccat ctatcactaa tgagagaacc tctggattgt
     5401 gatcaaatag attttcaata tagaaattta tattttcaga ttttcctttg agaaaatctt
     5461 tccaaaaatc gacacagtgc tctgcccgtt tttgagctgt tccagctttc ggaactcgtc
     5521 aaatgtgatg aattatactt ttagctcata gttgatttgc tgttttatag ccaagttcaa
     5581 agcggtttct tgcgacttct cttaccatag gatctgagct tcatggacat aaatctccaa
     5641 atggtgcatg cagagaaatt ggatttattc atttaatttt ttctttatga aaattgattt
     5701 gtgcctcatc ttcacacttt gtgggtttag aaaatagttg tacttctatt caaaatcaat
     5761 tgtttttagc ttttggggaa acagcttcta tgttcaaatt atcacaaaga atgatttgtt
     5821 tcatattttc agattatata ataaaaaata tttcttacct atgaaatagg tatattaaaa
     5881 ctttatttat tatcaagata aaaagataga tggtatatag acagtttcat taccatgaat
     5941 ttacaaattt ttgcatatta ttttttaaga gaatccaatt gtttttggat aattttcaca
     6001 tttttgtagt gatggattct gagcttatgg tggaaatttt gaacaataaa attactgaat
     6061 ctttgcggag attgttttcg ctgtcacaat tgagatagat atcaaattgt catcattgtt
     6121 gtaattgggt agatatagca caaattaaaa aatctcatct attattattt aatattacaa
     6181 caggtctttt tttgtatcat attcaatctg aaaatttcat aggacatagt actatatctc
     6241 atgggctata cgatgtcttc atataaatct tcttcatcaa gtaaaaattg gaaagctgga
     6301 ttgttcgtaa aattatttgt atcttgttct attttctttt ctctaatttt ggctgaatat
     6361 gggtataaac gatgatttgc aagatttata tcaataattt ccatatctgg agtagctttc
     6421 caagggatgt ctccatgact atattctgat atttctgaag cattcatgtt tttgaacttt
     6481 tcaattactt tgtttacatg agttttttgc tctcattcca ataaattgtc cgctactttt
     6541 tcattgatta tgtatctttg ctgtatatat cacatatatt ttgctgtaat agagataata
     6601 gactcttcct tttccattct attgataata aaatcaaaat tttctggagc tggtcatttt
     6661 ggtagtttaa taaaatctaa tcaggtaagt ctttctcatg tgagttcata tcggtcaaat
     6721 tcacaaaaat ataatatttt gtaaataaca gtttttcata cattggggag tgaagatgtt
     6781 ttatttagaa tatagagaat tagatttttt atttttttat attgtctatc gtttaatgtt
     6841 ttatgtttaa tttttttgtt attttgtaat gcagaaaagg atatatcaaa aatatcttgt
     6901 aaagatttga tatagggagt ccgtggagtt tttcagtttt caagtaaatt gtatgtcact
     6961 ctgcttattc aaagcatttc tgcaacttga tcttgtgtcc aattgttttt ttttctaagt
     7021 tttttgatga tgctatagtc cataatgttt gattatagtc aataaaaaat tatagatggt
     7081 tttattttag taatataaaa actattttca agttttttta aactgttttt attaaatatc
     7141 ttaacaaaaa aatgtattga aaagtttttg tttgtataaa atgatatatc aaaaatagca
     7201 ttaatattaa aattgtaatg aaatttttca attgttaaga aaatgagttg tagtgttaaa
     7261 ttagcattct caaaatagct tgaatactac tccgttggta attttagttt cagtaaaatc
     7321 tccaatttgt aatggttttt tggaagaaat gatgatttgt ttcatattat ctgtatatca
     7381 ttcatattga taatttcact tgtcatcctg tgctaaaaaa tcattgatca aagatgttct
     7441 aatatttcaa atctcttttt ggttatttga aagtgaaatt ttgt
//
LOCUS       DAOSID010000027         7062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_9681_length_7062_cov_13.717711, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000027 DAOSID010000000
VERSION     DAOSID010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7062
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9681_length_7062_cov_13.717711"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="PK674_01945"
     CDS             complement(<1..1082)
                     /locus_tag="PK674_01945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOG15327.1"
                     /translation="MLTSEIKRKIDNARDILVGKVPVPSQQIEQITIALIYKFMSDID
                     QEDKKLGGEGFFVGEYNKYSRFSIMDRKIGGQERLNLYTEGLEKMGINPHLPQMFRDI
                     FRNAYLPFKDPSTLDMFLKQIDEFSYDHSEDLGDAFEYLLSTAGSQGDAGQFRTPRHI
                     IDFIVSVVEPQLSDTVLDPACGTAGFLISAYKYIMAQHGDKKPGDKLSISQKKALTEN
                     FVGYDISYEMVRLSLVNMYLHNFGNPKIYEYDTLSSDDRRDDRFDCILANPPFMTPKG
                     GIQPHNRFSIKANKSEVLFVDYIIDHLNPRGKAGVIVPEGIIFQGANAYKSLREKMVK
                     ENYLWAVVSLPAGVFQPYSGVKTSILL"
     gene            complement(1102..3657)
                     /locus_tag="PK674_01950"
     CDS             complement(1102..3657)
                     /locus_tag="PK674_01950"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOG15328.1"
                     /translation="MQKEARARLKINDLLIKSGRRFFDEVKGKANVQVETNIKIFDEG
                     SELGDNFEKVKGGFVDYLLLDDKGFPICVLEAKSEDKDPLVGKEQARAYAHSQNVRFI
                     ILSNGNIHYFWDEKKDNPKRISYFPTLETLKSLETLESLDDFKPEPKKITDEVVDFDY
                     IARSQNPRFDEDPRRKDESKRGDYLYERGYRILRQYQLEAVRSVQNAISEGKNRFLWE
                     MATGTGKTLTAVALIKLFLRSGQAQRVLFLVDRLELEEQARKDLVKYLGNDYQTVVYK
                     ENKDDRRKAEIVVTTIQSLSYDNKYLKLFSPSDFDLVISDEAHRSISGSHRAIFEYFS
                     CYKLGLTATPKDYLKNLDKKKVAEEDPRQIERRQLLSTYVTFGCESGEPTFRYSLLDG
                     VKDGYLVNPISLDCRTEITTQLLSDEGYAVQQETDEGEEEIIYKGRNFERTFFSEETN
                     RSFCKIFMENAEKDPISGEIGKTIFFCVSRKHATKITQILNEMAHEMFPGKYNSDFAI
                     QITSDIPTAQQGTINFANDNLNGHSRFLEGYLSSKARVCVTVGMMTTGYDCQNILNLC
                     LARPIFSPSDFVQIKGRGTRTYTFKYAFKKGTETLQKEEKKTHFKLFDFFANCEYFEE
                     KYPYDEVLKLPKPGKKKTNTGGEITTIAGKFESEKFDPIKEIRKFVIGESGMRIDREL
                     YTSGFEKTVKEHFQTSKEFEEAVNSGDYEQMESYVRANIFDRPKEYYNIINLREGYKG
                     KTDRRLGLWEILDLIFGKIDKFKTKDDVAQEEFDKFIVSNHMIDQSKFYAAKEFFKSY
                     LINEMFRANVNNKNFREYADQPAIYDIFKVLGKDTMAELVDYIQDNVNEKNFY"
     gene            complement(3782..4351)
                     /gene="pth"
                     /locus_tag="PK674_01955"
     CDS             complement(3782..4351)
                     /gene="pth"
                     /locus_tag="PK674_01955"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOG15329.1"
                     /translation="MANDMKLIVGLGNPGEKYVKTRHNIGFFFLDQFVESNSFGDFVY
                     NNKYGGEVCVLTSPHPLLKGEGIDKVYFLKPMEYMNRSGQAVQKLMNFYKIDAKDLLV
                     IHDDIDLMVGKVQFKFGGGLAGHNGLKDIAEKLGTKDFARIRIGVDRPAIRSQVSDYV
                     LSSFKKEEIEKIQDRYLDFEKIIFEFINS"
     gene            4717..5718
                     /locus_tag="PK674_01960"
     CDS             4717..5718
                     /locus_tag="PK674_01960"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="CHAP domain-containing protein"
                     /protein_id="HOG15330.1"
                     /translation="MNTKLNFFALFTVLMIAGMFFVSCEKESMVLAEQPELNEPLFNM
                     KATSSVTYYSSPTVGNYTSNSSSSVTIGGNCGIHSGGILRAKLQSKSGNQFTVRISKQ
                     DGSNFGIAGTAYVKAASLCGGIAGQASYSAGVSYVDVTFYATFSRGIVNFYPMTIDGS
                     YGYRYYAEPILVYTSPMYTSNWVYGANLGSVNGANVYCNGTSNLSSGYYQCTEFCNRY
                     YSQVYGLNIINTGSQGGNANTWYGNASAKGLIAYANGGTIEPRPGDILCMSGGSQGYG
                     HVAIIIQVTSSFIKIAHQNSGTSWAPIGAQISRSGTTVSNPTGYTIQGWLRAPSYQY"
     gene            complement(5796..6956)
                     /locus_tag="PK674_01965"
     CDS             complement(5796..6956)
                     /locus_tag="PK674_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG15331.1"
                     /translation="MGKKLFLLALVVALSSLSITFGQNYSMEERMAYDYAYKNGITTM
                     NSIQSADMGGGLTRIAMAKMLSNYAINTLGLSPDTSKDCSFADVSTTLDTKYGNGVTK
                     ACQLGLMGVGIANFYPNGLVTRAEFGTVLSRALNAKDRTKLDKMNNASPYYAQHLKYL
                     KEQGIMNNISNSNSLERRGRVMTMLMRSDENSNNDSGRDLIEEGNGFTYRSKIVQRWN
                     GNNYRNEEYGFHLTLPETWNDCIVAHQQTVSKKTNKTEHEEFQLICPWEYAGDSNVNS
                     YIPNGYARLVDIVVVGPDTNDWTLSYLADQTLLENNKYKFGFGISDASKDIFDGKINA
                     SDLECGKYPNCTCHEKDSYLKGEYWDKVPDNYQEIGCFITTIVKDHIEIFDV"
BASE COUNT         2290 a         1267 c          938 g         2567 t
ORIGIN      
        1 agcaaaatag aagttttcac tccactataa ggttgaaata ctcctgctgg caaacttacc
       61 acagcccaaa gatagttttc ttttaccatc ttttctctaa gtgatttata ggcatttgct
      121 cactgaaaaa tgatcccttc tggaacaatc actcatgctt ttccacgagg atttaagtgg
      181 tctatgatgt aatccacaaa taaaacttca gacttatttg ctttgatgct aaatctattg
      241 tgaggctgaa ttcatcattt tggagtcata aagggaggat tggctagtat gcagtcaaat
      301 ctgtcatccc gcctgtcatc actactcaga gtatcgtatt cataaatttt tggatttcag
      361 aagttgtgca gatacatatt tactagagac aatctcacca tttcataact aatatcatat
      421 cagacaaaat tttcagtgag tgcttttttc tgagaaatac ttaatttatc tcatggtttt
      481 ttgtctccat gctgagccat aatgtattta tacgcactaa tcaaaaatca tgcagttcca
      541 caagctggat ccagaaccgt atctgaaagc tgaggttcaa ccacagaaac aataaaatca
      601 ataatatgcc ttggagtacg gaattgtcct gcatctcact gacttcatgc tgtagaaagt
      661 aagtattcaa aagcatctca taaatcctca ctatgatcat aagaaaattc atctatttgc
      721 ttcaaaaaca tatccaaagt agagggatct ttgaaaggca gatacgcatt cctaaaaata
      781 tcacgaaaca tttgaggaag atgtggattt attcccattt tctccagtca ttctgtatag
      841 agattcaatc tttcttgtcc tcaaattttc ctatccataa tactaaatcg tgaatacttg
      901 ttgtattctc aaacaaaaaa tcattctcct cctagtttct tatcctcttg gtcaatatca
      961 gacataaatt tataaatcag agctatcgta atttgctcta tttgttgaga aggaacaggg
     1021 actttcccta cgagaatatc tctcgcatta tcaatttttc ttttgatctc tgaagtaagc
     1081 atagtttagt agtgttttat attaataaaa atttttttca tttacattat cttgaatata
     1141 atctaccaat tccgccatag tgtctttacc taaaacttta aaaatatcat aaatagcagg
     1201 ttgatctgca tattctctaa agtttttatt gtttacattt gctctaaaca tttcatttat
     1261 tagataactc ttgaaaaact cttttgccgc atagaatttg gattggtcta tcatgtggtt
     1321 actaactata aatttatcaa actcttcttg tgccacatcg tctttagttt tgaatttatc
     1381 tattttacca aatatgagat ccagtatctc ccatagtcat aatctacgat cagtttttcc
     1441 tttgtatcat tctctaagat tgataatatt gtaatattct tttggtctat caaaaatgtt
     1501 agctcttaca taactttcca tttgctcata atctcaacta tttactgcct cttcaaattc
     1561 tttagaagtc tgaaagtgtt ctttaacagt tttttcaaat caagaagtat agagttctct
     1621 atcaatcctc attcagcttt caccaattac aaactttctg atttccttaa taggatcaaa
     1681 tttttctgat tcaaattttc atgctattgt ggtaatttct catcaagtat ttgttttttt
     1741 ctttcatggt ttcgggagtt tgagtacctc atcataagga tatttttctt cgaaatattc
     1801 acagttagca aaaaaatcaa acaacttgaa atgagtcttt ttttcttctt tttgtaaagt
     1861 ttctgttcat tttttaaaag catatttaaa ggtatatgtc cttgttcatc ttcattttat
     1921 ttgaacaaaa tctgaaggcg aaaatatcgg cctagcaaga catagattca aaatgttttg
     1981 gcaatcatat cctgttgtca tcattcatac tgttacacaa actcttgctt tactagataa
     2041 atatccctcc aaaaacctac tatgtccatt gagattatca ttagcaaaat ttatcgttca
     2101 ttgttgtgct gtaggaatat ctgaggtgat ttgtatcgca aaatctgaat tgtattttca
     2161 tggaaacatt tcatgagcca tttcattaag tatttgagta atcttagtag catgttttct
     2221 agatacacaa aagaaaattg tttttcaaat ctctccagaa attggatctt tttcagcatt
     2281 ctccataaaa attttacaaa aacttctatt agtctcttcg gagaaaaagg ttctttcaaa
     2341 atttcttcct ttataaatta tttcctcttc tcattcatcg gtttcttgct gaacagcata
     2401 tcattcatca gacaaaagtt gggtagtaat ttcagttcta caatctagag aaatcgggtt
     2461 tactaaatat caatctttga ctccatctaa taaagaatat ctaaaagttg gttctccact
     2521 ttcacatcaa aatgttacat aagtagagag taattgtctt ctttctattt gtctaggatc
     2581 ttcttcagca acttttttct tatctaaatt cttgagataa tcttttggtg tagcagtcaa
     2641 tcataattta taacaagaga aatattcaaa tatagctcta tgtgatcaac taatacttct
     2701 gtgggcttca tcagaaataa ccaaatcaaa gtcagatgga gaaaataatt ttaagtattt
     2761 attatcatag gatagtgatt gaatggtggt aacaactatt tcagcttttc ttcgatcatc
     2821 tttattttct ttgtagacaa cggtttgata atcatttcat aaatacttaa ctaagtcttt
     2881 tcttgcttgt tcttctaatt ccaatctatc aactaaaaat aatactcttt gagcttgtca
     2941 tgatcttaaa aaaagcttta ttaatgcaac agcagtgagt gttttccctg ttcaagttgc
     3001 catttcccat agaaatctat tttttcattc actaatggcg ttttgaacag atcttacggc
     3061 ttctagctga tattgtctta gtattctata tcatctttca tataaataat ctcatctttt
     3121 actttcatct ttccgtctag gatcttcatc aaaacgagga ttttgactcc ttgcaatata
     3181 atcgaaatca actacttcat cagtgatttt ctttggttct ggtttgaagt catccaaaga
     3241 ttctaatgtt tctaaagatt ttaatgtttc taatgtaggg aaatatgata ttctttttgg
     3301 attatctttt ttttcatccc aaaaataatg aatattccca tttgataaga taataaatct
     3361 aacattttga gaatgagcat aggctcttgc ttgttctttt cataccaaag ggtctttgtc
     3421 ttcgcttttt gcttctagta cacaaatagg aaatcactta tcatccaaga gcaaataatc
     3481 tacaaaacca cccttcactt tttcaaaatt atctcataac tcacttcatt catcaaaaat
     3541 tttaatattt gtttctactt gaacatttgc ttttcatttc acctcatcaa aaaatcttcg
     3601 tcatgattta ataagtaaat cattgatttt caatcttgct ctagcttctt tttgcatttt
     3661 tagaataatc ttgaataaat ttctgagagt atatattttt ccacctcctt ttcaaaggaa
     3721 gattttttat ttacataaaa aacatttctt ccgataatta aattccccca aaaaaaacaa
     3781 attaactatt tataaattca aaaataattt tttcaaaatc taaataccta tcttgaatct
     3841 tttcaatttc ttcttttttg aaactactca acacataatc tgaaacttga gatcgaatag
     3901 ctggtctatc tactcaaatt ctaatcctag caaaatcttt tgttcacaac ttctcagcaa
     3961 tatcttttag tcaattgtga ccagccaatc ctcatcaaaa cttgaactga acttttcaaa
     4021 ccatcaaatc tatatcatca tgtattacca acaaatcctt agcgtctatt ttataaaaat
     4081 tcatcaactt ttgcacagct tgaccagacc tattcatgta ctccataggt ttcaaaaaat
     4141 acaccttgtc aattccctct ccttttagga gagggtgggg tgaggttaat acacaaactt
     4201 ctcatccata cttattatta taaacaaaat ctcaaaaact atttgattct acaaattgat
     4261 ccaagaaaaa aaatcaaata ttatgtctag tttttacata tttttcacca ggatttccca
     4321 gtcatacaat caatttcata tcatttgcca tttttaatat acaaaaaaac taaaaatccc
     4381 tttcgtttac tactaaaata aatattttga aaaaaaaatc aatgaaatgt tatgttatga
     4441 ttaaattttt atctcactag aatcttaatc tcactagatt tctctcttga atttcatttt
     4501 caaataaata tagttcaaaa attcgagatc tgaatataga ttttcaaaaa aaagtaaatt
     4561 ttctccaaga gaagacgaaa tccgaagtgt gatgactttg ttactcaaca tccaaagaaa
     4621 gttactttgt aaaaaaccgt agtaaatagg actactaata aagaaagctc tgattctttc
     4681 ctattgagag ttagaaaaaa caaaaaaaat tacaaaatga atacaaaatt gaattttttt
     4741 gcactgttta cagtgctaat gatagcagga atgtttttcg tttcctgcga aaaggaaagt
     4801 atggtgctag ccgagcaacc agaactaaac gaaccactat tcaatatgaa ggctaccagt
     4861 tctgtaacct actattcatc tccgactgta ggcaactata catcaaatag tagctcttct
     4921 gtcacaattg gtggcaattg tggcatacac agtggtggta tccttcgagc gaagcttcaa
     4981 agtaaaagcg gaaaccaatt tactgtcaga atttcgaaac aagatggtag taattttggc
     5041 atagctggta cagcatatgt aaaagctgct tcattgtgtg gtggtatcgc tggacaagca
     5101 tcatactctg ctggagttag ttacgtagat gttacatttt atgcaacatt ttctagagga
     5161 attgtcaact tctatccaat gactattgat ggctcttatg gctatcgata ctacgctgaa
     5221 ccaattcttg tctatacaag tccaatgtat accagtaact gggtttatgg tgcgaaccta
     5281 ggaagtgtca atggtgctaa tgtttattgt aatggaacat ccaatctttc cagtggttat
     5341 taccaatgca cggaattttg caacagatat tatagccaag tgtacggatt aaacattatt
     5401 aacactggct ctcaaggtgg taatgcaaat acatggtatg gcaatgcatc agcaaaagga
     5461 ctaatagcat atgcaaatgg tggaacaatt gagccgagac ctggcgacat attatgtatg
     5521 tcgggtggat cacaaggcta cggacatgta gcaattatta ttcaagtgac aagctcattt
     5581 ataaaaattg ctcaccagaa ttctggaaca tcgtgggctc ccattggtgc tcaaatatct
     5641 aggagtggaa caaccgtttc taatccgact ggttatacta ttcaaggctg gctacgagcg
     5701 ccatcatatc aatattaatt gtagttttct tcaaaaaaag gtctttttat gagacctttt
     5761 tcttaaataa caatatatat taatgatagt ataaattaaa catcaaatat ttcaatatga
     5821 tcctttacta tagttgtaat gaaacatcaa atttcttgat aattatctgg tactttatcc
     5881 caatattctc atttcaaata actatctttt tcgtgacaag tacaatttgg atattttcag
     5941 cactctaaat cactcgcatt aatttttcaa tcaaagatat ctttacttgc atcagatatt
     6001 ccaaatcaaa atttatattt attgttctcc aataatgttt gatctgctag atagctcaaa
     6061 gtccaatcat tagtatcagg tcatacaact acgatatcta ctaaccttgc atatcagttt
     6121 ggaatatatg aatttacatt gctatctcat gcatactccc atggacaaat taactgaaat
     6181 tcttcgtgtt cagttttatt tgttttttta ctaactgttt gttgatgagc tactatacaa
     6241 tcattccaag tttctggaag agttaaatgg aatcaatatt cttcatttct ataattattt
     6301 caattccacc gttgcacaat ttttgaacga taagtgaatc cattaccttc ttcaattaaa
     6361 tctcgtcacg aatcattatt gctattttca tcacttctca tcagcatggt cataactcgt
     6421 catctgcgct ctaaactatt tgaatttgaa atattattca taattcattg ctctttcaag
     6481 tatttcaaat gctgtgcata gtaaggactt gcattattca ttttgtctaa ttttgtccta
     6541 tcttttgcat ttaatgctct tgagagtaca gtcccaaatt ctgctctcgt aaccagacca
     6601 tttggataaa aattagcaat ccctactcac atcaaaccta attgacatgc ttttgtcact
     6661 caattaccat attttgtatc taaagtagta ctgacatcag caaaagaaca atctttacta
     6721 gtatctggac tcaatcacaa tgtatttata gcataatttg aaagcatctt tgccatagca
     6781 atcctagtaa gtcatcatca catatcagca ctttggattg aattcatagt agtaattcca
     6841 tttttatagg catagtcata agccattctt tcttccatag aataattttg tcaaaaagtt
     6901 atagatagag aggacaaagc cacaactaaa gccaaaagaa ataatttttt tcacattatt
     6961 ttatatttta taaataaata tgataacaca tgaaacatgc atgaaacatg tatatataaa
     7021 attacttaat tttcaaacaa tttttgtttt ccttggttaa tt
//
LOCUS       DAOSID010000028         6840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_10089_length_6840_cov_14.991452, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000028 DAOSID010000000
VERSION     DAOSID010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10089_length_6840_cov_14.991452"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1523
                     /locus_tag="PK674_01970"
     CDS             69..1523
                     /locus_tag="PK674_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15332.1"
                     /translation="MFKKIFSYIISLTLFVTTSFSFTSAYLFGIKSFDNTIDNIQKIE
                     SKHKFNFPIVAFIFDPRDEGQMLDILNNLSDKFGANKIYHITLSPNNYSAKQVADGKF
                     DNQYTIFFQLIKRNNLKVIFRTMHEMNGGWYPRGSNPDTFKEARIHVRNLSRKLGLDQ
                     NNILFDMSVNHRDMPTKSTPSQTAFLITCDQKSKFTEIKHQIFVSTGRKTEIIEKKVP
                     IAQSQLDKLLNRPIKYNIIKDTVQIEYPIYKVETEKQQNCYTFEDYYPGNKYVDILGV
                     TFYNRGKASYNRHRLLPEQILNDKDWDTLKRLKSFKKPIFIDEVATTAVRYPGAYNQE
                     LSRQSYRNDYDLKNQRLVSLKDFMLKTPEILGMIYFNIDYTYGLTHWMIGEADRAIIN
                     LNSNKFYSATCDLYNHQSNNKKLLNLFNSKKDLDPSVDQNARLLADLLIEKFGVEESI
                     KRIEIISLKSKSVALNNLLGDVLHLLKIAGKGQK"
     gene            complement(1742..1826)
                     /locus_tag="PK674_01975"
     tRNA            complement(1742..1826)
                     /locus_tag="PK674_01975"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1790..1792),aa:Ser,seq:gga)
     gene            complement(1837..2541)
                     /locus_tag="PK674_01980"
     CDS             complement(1837..2541)
                     /locus_tag="PK674_01980"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOG15333.1"
                     /translation="MNKYLAGLALLGGLTYFGCCKGQFFDKTFSKIKSQKELTIDTSK
                     KLIRNNVLFTIDDGPSIYANEIAKTLDSLGYQGIFFVVTRGINQKTKGDLIKILKMGH
                     HIGNHSYSHPNFQNLNLEEIKYQILQSDSIISSIYKEAGIKWEKKYIRYPYGNPPIKK
                     DIDEINNLLDSLNYQKPMFRHMNTDLLDRKSSPSDEKVNQMKDGDTILLHERKYTPQT
                     ISRIVNSIDNKKENNK"
     gene            complement(2664..3029)
                     /locus_tag="PK674_01985"
     CDS             complement(2664..3029)
                     /locus_tag="PK674_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOG15334.1"
                     /translation="MFGKVGEMKKMYDKYKALQKALQNLVIRAKEGKYMLDGEEQEGA
                     LVVDISGEMKLRDVSINDQSLLSPEKKTELEELIVKTFQKAQNKAQEIVAEKTKEILG
                     FDPNDMASMMAGGGIPGLS"
     assembly_gap    3152..3251
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3404..4240)
                     /locus_tag="PK674_01990"
     CDS             complement(3404..4240)
                     /locus_tag="PK674_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15335.1"
                     /translation="METINLIEFIKNGEIFEKMDKGFEQIVSDFDFGKLENFVHIKSF
                     TDFKIINQKKKTLFILDHLLLDNVIDYLKDITEYSILDLNFGSIGFSKKIGISQVGIL
                     QLVALGIEIYEPFDLSSFLSNLDSSRKKYFRINNFDLPENLRDIYDNTDMVSLEEYGF
                     EGGNFAIITTGSMLIEVLRLGELLQADGLEFQIFILNRLDKLESSTFGNIKNLIFIFD
                     GLNFKYEYLIKKVFGDKNLKFITPKYNKLSTILDDYIMENLEFDAQSLKNRLKKSGIE
                     GL"
     gene            complement(4318..6534)
                     /locus_tag="PK674_01995"
     CDS             complement(4318..6534)
                     /locus_tag="PK674_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="penicillin-binding protein 2"
                     /protein_id="HOG15336.1"
                     /translation="MSQSFLKAERGDILAYDKSHNEVKLTENISIYNVFIDPKYIGDK
                     ERFIELFWPIAYTHLCEINGMEKVEKIDCIKNLEIFAKKDLLPKTPEFFYMGSGIMSD
                     GYDSYDWTGFYEQTEAILSGFNTGTAETLIRGALDKRIQVGIKKENYIGFFSNKNFLA
                     ELEGLGLKYINIKYNNYVYIVPTKIANVSREGAPLKKLLAKYDYLNNYPDFDKNFYQQ
                     EYRYIKLLSDVNPVVAQMVKDLKIKHYNETTRDRIPILHGLGLEPFVKRYYPYGSFLS
                     NVLGYVDKNGVSFYGIEQYFDDMLRGKDGKIIGRASAWIGGVGANEFEIEDVINGDDV
                     YLTIDIGIQKEVELMAKKRQESLQADSVSILIYDPNNGQIKASINYPSFDPNNYNDVY
                     MLKPLGIDESFIVDNDTYMDVPIYIISGGETKLATTKERVNIELKKYVTKNIYGPQVF
                     VDKNIAMAYEPGSIFKAFTVAIGLDTDEIRLYDFYHDPGEVKVGPYTIKNADNKNCMG
                     DKSFMNAFTYSCNIGMVRIVQAVGKNNFYNYLNKLNFGQMTNIELAGEDPGSMDLVTV
                     VSMARFLNNSFGQGLLSTPIQLAAAYSAIVNGGHYIKPTIIAGTYDKQTGKYHENKTQ
                     ILRQIFKPETAEEIKEALFMIMETNPDYVKMMRVEGFTLGGKSGTSQISFRGKYMQGL
                     GWTNTSFIGLVTKENPEYVVIVQVRRPRATIWGAQTAGKVFSEVGKFLIGYSFIEK"
BASE COUNT         2343 a         1044 c          831 g         2522 t
ORIGIN      
        1 taaacttata atttttatac aaaatatttt atataagttt ttagataaaa taattttctt
       61 gattacacat gtttaaaaaa atattttcct atatcatatc tctaacttta tttgttacta
      121 ctagcttttc gttcacaagc gcttatcttt tttgaattaa atcatttgat aacacgatcg
      181 acaatatcca gaaaatagag tcaaaacata aatttaattt ccctatagtt gcttttattt
      241 ttgaccctcg agatgagtga caaatgttag atatactaaa caatttaagt gataaatttg
      301 gggcaaataa aatatatcat atcacactat ctccaaacaa ttattctgcc aaacaagttg
      361 cagattgaaa atttgataat caatatacaa ttttttttca gctgataaaa aggaacaact
      421 taaaggtaat ttttagaaca atgcatgaga tgaatgggtg atggtatcct cgatgatcca
      481 atccagatac tttcaaagaa gcacgaattc atgtccgaaa tttatctaga aaattatgat
      541 tggatcaaaa taatattctt tttgatatgt ccgtaaatca tcgagatatg cccacaaaat
      601 ccacaccatc gcaaacagct tttttgatta catgtgatca aaaaagtaag tttacagaaa
      661 taaaacatca gatttttgtt tcaacaggta gaaaaactga gattatagaa aaaaaagttc
      721 caatagctca aagtcaactt gataagttat taaatagacc aatcaaatat aatattataa
      781 aagatactgt tcaaatagaa tatcccattt ataaagtaga aacagaaaaa caacaaaatt
      841 gttatacttt tgaagattat tatccgggaa ataaatatgt agatattctt tgagtgactt
      901 tttataaccg atgaaaagct tcgtacaata gacatcgatt gttaccagaa caaatactaa
      961 atgataagga ttgggatact ctaaaaagat taaaatcttt caaaaaacct atttttatag
     1021 atgaggttgc tactacagct gttcgatatc cttgagcata caatcaggag ctttccagac
     1081 aatcgtatag aaatgattat gatctaaaaa atcaacgact agtttcatta aaagacttca
     1141 tgttgaaaac accagaaata ctgggtatga tttacttcaa tatcgattat acatactgat
     1201 tgactcattg gatgatatga gaagcagatc gagcaattat caatttaaat agcaataaat
     1261 tttattcagc tacctgtgat ctttacaatc accagtctaa taataaaaaa ttgttaaatt
     1321 tatttaatag taaaaaagat ttagacccct ctgttgatca aaatgctagg ttattggccg
     1381 atttgttgat agaaaagttt ggagtagagg agtctataaa aagaatagaa ataatatcac
     1441 ttaaaagcaa gagtgttgct ttaaataatt tattaggtga tgttttacat ttattaaaaa
     1501 tagcatgaaa aggacaaaaa taatctctac aataattgag aattatcaag tatgataata
     1561 attttttaag caaaaagcta taagaaatta tcttgcggtt ttttgaagtt taaaataatt
     1621 taagaattct ttacggaata caaggtttga ttatgtttaa tgagaagtta atgcttgata
     1681 agcatgttct ctacacttta ttagaatata ttttggataa aaattttagt atcaaaaaaa
     1741 tcggaagatg ggggattcga acccccgtta ccttgtggta aacaccgttt ccaacggtgc
     1801 gcattcaacc gctctgccaa ccttccaatt tgtttgttat ttattgtttt cttttttgtt
     1861 atcaatagaa tttacgattc ttgatatagt ttgtggagtg tattttcttt catgtaataa
     1921 tatcgtatca ccatccttca tttgattaac tttttcatca ctaggacttg attttcgatc
     1981 aagcaagtcg gtattcatat gtcgaaacat aggtttttgg taatttaatg agtctaatag
     2041 attgtttatc tcatctatat ctttttttat cgggggatta ccataaggat atctaatata
     2101 cttcttctcc catttgattc atgcctcttt gtaaatagag cttattattg aatcagattg
     2161 tagtatttga tattttattt cttctaaatt taaattttgg aagtttggat gagaataact
     2221 atgatttcat atatgatgtc acattttcaa tatttttata agatctcatt ttgttttttg
     2281 atttattcat cttgttacaa caaaaaatat tcattgatat catagtgaat ctaatgtttt
     2341 tgctatttcg tttgcataaa tgctaggtca atcatctata gtaaaaagca cattatttct
     2401 aataagtttt ttgcttgtat ctattgtaag ttctttttgt gattttattt ttgaaaaagt
     2461 cttatcaaaa aattggcctt tacagcatca aaagtatgtg agacctcata aaagagctaa
     2521 tcctgccaaa tatttattca taactttttt aaaaaataaa tattgaattt attcatttaa
     2581 tgagagtgac ttgatgaatt acttatcaac aaaaaatcac tattttgggt taataatgtt
     2641 ttgtttttat tgattgtttg taattagctt agtcctggaa tacctccacc agccatcata
     2701 ctagccatat catttggatc aaatccaagg atttcttttg ttttttctgc tactatttct
     2761 tgagctttgt tttgtgcttt ttgaaaagtt tttactatca attcttctaa ttctgttttt
     2821 ttctcaggag ataatagaga ctgatcattg atactaacat ctcttaattt catttctcaa
     2881 cttatatcta ctactagagc tcattcttgt tcttctcaat ctaacatata ttttcattcc
     2941 ttagctctaa ttactaaatt ttgtaaagct ttttgaagag ctttgtactt gtcatacatt
     3001 tttttcattt ctccaacttt tccaaacatt tttgcttatt tataatctaa aaaacaacaa
     3061 aaccatagtt tcatcatgaa acacacttaa aaatattatt aatgattaaa agtttttttt
     3121 caagaagatt agagtctttt tattcgtata annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn ntattaatga ttaaaagttt tttttcaaga agattagagt ctttttattc
     3301 gtataacaaa gaatagttgg ttcaagcata agttagtata gggaatggtc gtggtcattt
     3361 ttatattaac aattataaac tattaccata ttccaaaaaa tttttacaat ccctctatcc
     3421 cacttttttt caacctattt ttgagacttt gtgcatcaaa ctctaaattt tccattatat
     3481 agtcatccaa gatcgtagaa agtttattgt atttcggtgt gataaatttc agatttttat
     3541 ctcaaaaaac tttttttata agatattcat acttaaaatt tagcccatca aaaataaaaa
     3601 ttaaattttt tatgtttcaa aaagtactag actccaattt atctaatcta ttcaaaataa
     3661 aaatctggaa ttctaatcca tctgcttgta ataactcacc aagtctaaga acttcaatca
     3721 acatcgatca agtcgtaatg attgcaaaat ttcaaccttc aaaaccgtat tcctccaacg
     3781 aaaccatatc tgtattatca taaatatccc gtaaattttc tggtaaatca aaattattta
     3841 ttctgaaata ctttttccta gatgaatcta agtttgacaa aaatgatgat aaatcaaatg
     3901 gctcatatat ttctattcat aaagctacta attgtaatat gcctacttga gatattcata
     3961 tttttttact aaatcctata ctaccaaaat tcaaatctaa aatagagtat tctgttatat
     4021 cttttaaata atctatcaca ttatctagta gtaaatgatc taagataaat aatgtttttt
     4081 tcttttgatt tattatttta aaatctgtaa aagatttaat atgcacaaaa ttttctaatt
     4141 ttcaaaaatc aaaatctgaa actatctgtt caaatccttt atccattttt tcaaaaattt
     4201 ctcaattttt aataaattct atcaagttaa tcgtttccat cacacaagaa agttgaaagt
     4261 taaaagttaa gtaccacccg ccccatagga ctacggggca ggtagtaggg gttgtggcta
     4321 tttctctata aatgaatatc caatcaaaaa ttttccaacc tcagagaaaa cttttcaagc
     4381 tgtttgtgct caccaaattg tggctctagg cctacgaact tggacaatta caacatattc
     4441 tggattttct tttgttacca atcctataaa tgaggtgttt gtccagccta aaccttgcat
     4501 atattttcat ctaaatgata tttgagaagt tcaacttttc cctcaaagag taaatcactc
     4561 tactctcatc atttttacat agtcaggatt ggtttccata atcataaata gggcttcttt
     4621 gatttcctca gcagtttctg gtttaaaaat ctgtctcaat atttgagttt tattttcatg
     4681 atattttcaa gtttgtttgt cataggttca tgcgattata gtcggtttaa tatagtgacc
     4741 accatttaca atagcactat aagctgctgc tagttgaatt ggagtactta ataatccctg
     4801 tcaaaatgag ttattcaaaa atcttgccat tgaaaccaca gtaaccaagt ccatagaacc
     4861 aggatcttct catgccaact ctatgtttgt catttgacca aaatttaatt tatttagata
     4921 attataaaaa ttatttttcc ccactgcttg tacaattctc accattcata tattacaaga
     4981 ataggtaaat gcattcataa aagacttgtc tcacatacag tttttattat cagcattttt
     5041 tatagtataa ggtcaaactt tcacctcccc aggatcgtga taaaagtcat acaaacgaat
     5101 ttcatccgta tccaaaccga ttgctacagt aaatgcttta aaaatacttc caggttcgta
     5161 agccatggct atgtttttat caacaaaaac ctgtgggcca taaatatttt ttgttacata
     5221 ttttttaagc tcaatattaa ccctttcttt tgtggtagct aatttagtct ctcatcagct
     5281 tataatataa atcggaacat ccatataagt atcattatcc acaataaacg attcatcaat
     5341 tcccaaaggt ttgagcatat acacatcgtt gtaattattt ggatcaaatg aggggtagtt
     5401 gatagaggct tttatttgac cattatttgg atcataaata agaatactca cagagtcagc
     5461 ttgcaaactt tcctgtcgtt tttttgccat caactctact tctttttgaa ttcatatatc
     5521 tatagttaaa tagacatcat ccccattgat tacatcttct atttcaaatt catttgctcc
     5581 aactcatcca atccaagctg aggcacgacc aattattttt caatcttttc aacgcaacat
     5641 atcatcaaaa tattgttcta ttccataaaa ggaaactcaa tttttatcaa cataccccaa
     5701 cacattggac aaaaaagacc cataagggta atatcttttt acaaaaggtt ctaatcctag
     5761 tccatgcaat attggaattc tatccctcgt tgtttcatta taatgcttta ttttcaaatc
     5821 tttaaccatt tgtgctacta ctggatttac atcagacagt agtttaatat atctatattc
     5881 ttgttgataa aaatttttat caaaatcagg ataattgttc aaataatcat atttagctaa
     5941 aagttttttt aatggagctc attctctaga aacatttgca attttggtgg gcactatata
     6001 aacatagtta ttatacttta tatttatata tttcaacccc aatcattcca attcagccag
     6061 aaaatttttg ttagaaaaaa atcaaatata gttttctttt tttatcccta cttgaatcct
     6121 tttgtccaaa gctcatctta tcaaagtttc ggctgttcaa gtattaaatc cagataatat
     6181 agcttctgtt tgttcataaa atcctgtcca atcatagcta tcatatccat cgctcattat
     6241 tccgcttccc atataaaaga attcaggagt ttttggcaaa agatcttttt ttgcaaaaat
     6301 ttcaagattt ttgatacaat ctattttttc tactttttcc ataccattta tttcacatag
     6361 atgtgtgtaa gctatcggcc aaaatagctc aataaagcgt tctttatctc aaatatattt
     6421 tggatctata aaaacattgt atatagagat gttttcagtc aactttactt cattatgaga
     6481 tttgtcgtaa gctaaaatat cccccctttc tgcttttaaa aaagattgac tcatgtgttg
     6541 tctatttaaa atatcatcat attttttgtg atgaataatt tgaacaaaaa aaagtctaat
     6601 aagaagtaca agaaataaaa acagaaatat caaaaaaata taaaattccg cagttcaata
     6661 gaaatctttt aattttttat tgtatttaaa tttatttgtt ataaaaattt ttaacataaa
     6721 aaaatattga ataaacacaa aaaatttaac aaaatgattt aagttttcaa tacttaatca
     6781 ggggttatta attataattc aaaaagcacc actaatagat aagataataa atgggaattt
//
LOCUS       DAOSID010000029         6740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_10271_length_6740_cov_13.099925, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000029 DAOSID010000000
VERSION     DAOSID010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10271_length_6740_cov_13.099925"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..>1666)
                     /locus_tag="PK674_02000"
     CDS             complement(24..>1666)
                     /locus_tag="PK674_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15337.1"
                     /translation="KKIEEEKKKIEEENKQEEIIKLKKQKLDDLYKNNIIDKFKGSSE
                     ELVVFQKKIIKEYSIKGVDIIGKLNKNKFDVTSYFKYLFTAQKLLEDNEAPIENKYEF
                     IKSFNDLNKSLDIDIQLEMEAYFADQKKKFKIDIFAKKQSISLVALAEGERILDNKEF
                     QKFKNSQKHNIENIDLGKNKKSILELYSNDKKNLTQIIDYINDDGSINEQKIDEIIKN
                     PDEKSKIIKNLNSQIKESLERYQQDLQVITEQKIKERIISNCVKSVSNIFNQTTENME
                     NFAKDFKIDTDSGVNFNSENQEVYIKGNINGKHVGLYYNISTGIVEMDDFMGYDSNEN
                     GGTYIMGKENGKREKLKFNLPTYNQLLNETKNVDFSQIIDYSRNILDYKENLGSKLDE
                     KISSQFKNKSILQYYVEKFNEKNIAEQTVLEDVFSSWGNYDKSIVDFDKKTLISKDLN
                     QNGYSLMEIIFNTLEGNTRTMENKMDADNLRRFRNCINKFNMIINTNQVRNGESEEPL
                     LSLFDEKNMKDSAKNFKGGGDLNYLDFFNNIGNSKIGF"
     assembly_gap    1667..1766
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1767..2220)
                     /locus_tag="PK674_02005"
     CDS             complement(<1767..2220)
                     /locus_tag="PK674_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15338.1"
                     /translation="MGNGLFIDKSYTTEKRDTPDFFKSSKKGPILHSDESIQIDPNQD
                     FIQEVGEKLQKEGLYNFDIDTYNYIAQNVLLKLNKKYGDNKGAIIFNKYLEYIIIKVI
                     KEFNNAFVDGDYTVFCNGIEDILLTEEEKKKTEEEKKKTEEEKKKTEEE"
     assembly_gap    2385..2484
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2485..3069)
                     /locus_tag="PK674_02010"
     CDS             complement(<2485..3069)
                     /locus_tag="PK674_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15339.1"
                     /translation="MNNKLRQGLIISTIVILGLLGQVKEAIAQTLNDKNIDKTEELAR
                     ITDNTASAINMNFDLDRSSPDVSAVKQFFMTPGFLSEIGLRDFNTIWDKAAKSLIWPY
                     REEAILDIFSRERNDFEKVVLILAILDPTGETDLGERVIKEISNNKLYQYKMKQIISI
                     MEKKQSILEIDERIQKTDERIQKTDERIQKTDERI"
     gene            complement(3160..4953)
                     /locus_tag="PK674_02015"
     CDS             complement(3160..4953)
                     /locus_tag="PK674_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15340.1"
                     /translation="MFEINDNYIAHTDFIDNNIDEVDSVKGKKIEDNDQKFLKYYKIT
                     NDIIKTVSNQTRFSGLGIDISHIGNDVFHNLRNNERDIFEYDEELVVAMVENEITNRL
                     LIEERNRAKQIKTGEVSINKDKEQGNKDKEQGNKDKLEKINTLVGGLKTLYSGLLPEL
                     KRTDTQELPDLGNITLNTESITKTEFAQYLFSYNKIQEKRNQGEILTENEEKFMETFK
                     NFTKELGIEIAIAIPINSERPKTVEKKLVVRPTSLENIANSDAILENKRTKGFVAKRL
                     ENSSSGKVSLSLDNEKDLSRLPKYAVKKFKEVFLRNKDLFDKYKDFVNDDGTLKNSDD
                     TLLTAIKEYIAQYEEEKKKDILRKIHERAMIGCFRGLTTYFDINSINQENFIKDIKLN
                     INDDITVDEKNKLYIRGSIKGKNIGIYYNMDDGTVEMDDFLTKDENGEFFIGANHGKR
                     EKLAFTLPTYDSLVDKGGQIDFSGILKKNDREKDIEEQIDKSLDRTMDSSFKNENINK
                     FFVERHMEEYIAEQEVLDSIFGNRFEGVNYRTATDFRMDDFNIKTNENNRYKLVSMIY
                     NTIEEQKTPSDIRRLRDGINKLNYFIEKAKD"
     gene            complement(5053..5718)
                     /locus_tag="PK674_02020"
     CDS             complement(5053..5718)
                     /locus_tag="PK674_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15341.1"
                     /translation="MNNKLRQGLIISTIVILGLLGQVKEAIAQTLNDKNIDKTEELAR
                     ITDNTASAINMNFDLDRSSPDASAVKQFFMGGDILTEEEKVEFDMLWNKAMQSNVRDF
                     YEKQILKFFNGKGYTDEQKKIFVSAFLDQTQTTKLGQYAFKEISQNRVYQFRLSQIIK
                     QVELEVDIAKLDREIIEERIKGKELDQKGKELDQKGKELDVLLDALKNMEEAFKQYQT
                     NNK"
     gene            complement(5802..6404)
                     /locus_tag="PK674_02025"
     CDS             complement(5802..6404)
                     /locus_tag="PK674_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG15342.1"
                     /translation="MRTGLTKFQRCPGCGNFIIHLAIKQALAELKIPKHKTVVVTGIG
                     CSGKMSQYVDGYGAETLHGRGIPFATGVKLANPDLTVISVSGDGDTYGIGLGHLLHSA
                     RKDINIVHIVADNENYALTTGQASPTTPENIKTKSTPDGNTISPFDPLKLTESAGCKL
                     NISANDRDLPGLKKAIMDGIQHKGFSHINVKQICPSFKKW"
BASE COUNT         1966 a         1051 c          635 g         2888 t
ORIGIN      
        1 tccatatcac gaacaaaaaa tacctaaaat cagatttttg aatttcctat attgttaaaa
       61 aaatctaaat aatttagatc tcatcaacct ttaaagtttt tagcactatc cttcatgttc
      121 ttttcatcaa acaatgataa caatggttct tctgattctc aatttctaac ttgatttgta
      181 tttataatca tattaaattt atttatacaa tttctaaatc ttctcaaatt gtctgcatcc
      241 attttatttt ccatagttct tgtgtttcat tctaatgtat taaaaataat ttccattaaa
      301 ctatatcaat tctgatttag atctttggat ataagtgttt ttttatcaaa atcgactatt
      361 gatttatcat aattccccca actagaaaaa acatcttcta atacggtttg ttcagctata
      421 tttttttcat taaatttttc tacataatat tgtaatattg atttgttttt gaactgactt
      481 gagatttttt catctaactt agatcataaa ttttctttat aatctagtat gtttctagaa
      541 taatctatta tttgtgagaa atctacattt tttgtttcat ttaaaagctg gttatatgtt
      601 ggcaaattaa acttcaattt ttctctctta ccattttctt ttcacataat ataagttcat
      661 caattttcat tactatcata tcacataaaa tcatccattt caacaattca agtacttata
      721 ttataatata atcaaacatg ttttcagttt atgtttcatt ttatatatac ttcttggttt
      781 tctgaattaa aattaactca gctatctgta tctatcttaa aatcttttgc aaaattttcc
      841 atattttcag ttgtctgatt gaaaatatta gaaacagatt taacacaatt tgatattatt
      901 ctttctttta ttttttgttc tgttattacc tgtaaatctt gctgatatct ttccagtgat
      961 tcttttattt gactattcaa attttttatt atcttagatt tttcatctgg attttttatt
     1021 atctcatcta ttttttgttc atttatagat caatcatcat ttatataatc aataatttga
     1081 gttagatttt ttttatcatt ggaatacaat tctaatattg attttttatt tttacccaaa
     1141 tctatatttt ctatattgtg tttttgtgaa tttttaaatt tttgaaactc tttgttatct
     1201 aatattcttt ctcattcggc aagagctact aacgatatag attgcttttt tgcaaaaata
     1261 tcaattttaa atttcttttt ttgatcagca aagtaagctt ccatctctag ctgaatatct
     1321 atgtccaaag acttattgag atcattaaag gattttataa actcatattt gttttctatt
     1381 ggagcctcat tgtcttctaa taatttctga gctgtaaaaa gatacttgaa ataagaagtg
     1441 acatcaaatt tatttttatt taactttcat attatatcta ctcacttaat agagtattct
     1501 ttgataattt ttttttgaaa aacgactaat tcctcactac ttcatttaaa tttatcaata
     1561 atattgtttt tatataaatc atctaatttt tgctttttta acttaataat ttcttcttgt
     1621 ttattttctt cttctatttt ctttttttct tcttctattt tcttttnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnntttc ttcttctgtt ttcttttttt cttcttctgt
     1801 tttctttttt tcttcttctg ttttcttttt ttcttcttct gtcaaaagta tgtcttctat
     1861 accattacaa aaaacagtat aatctcaatc tacaaatgca ttattaaatt cttttattac
     1921 ttttataata atatattcta aatatttatt aaaaataata gctcatttat tatctcaata
     1981 ttttttattt aattttaata aaacattttg tgctatataa ttatatgtat caatatcaaa
     2041 attatataat cattcttttt gaagtttctc tcaaacctct tgtataaaat cttgattagg
     2101 atctatttgt attgattcat cactatgcaa aataggtcat ttttttgatg atttaaaaaa
     2161 atcaggtgta tctctctttt ctgttgtata acttttatct ataaataatc aattacccat
     2221 aaaaagttgt atattaatta aaactacaac tattatacat aaaattatgt gttttgtcaa
     2281 aaaaaagaca tattaattta tgtccccttt tcaaatatgt gttcccaaga ctctaattct
     2341 ttaataaatt gttttaattc ttgaattctt tcatctcttt tttgnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnaattct ttcgtctgtt ttttgaattc tttcgtctgt
     2521 tttttgaatt ctttcgtctg ttttttgaat tctttcgtct atttctaata tagattgttt
     2581 tttctccatg atacttatga tttgtttcat tttatactga tataatttat tgtttgatat
     2641 ttcttttata actcgttctc ctaaatctgt ttctcatgtt ggatctaaaa tagctaatat
     2701 taatacaact ttttcaaaat cgtttctttc tcgtgaaaat atatctaaaa ttgcttcttc
     2761 acgatatggc caaataaggc ttttagctgc tttatcccat attgtattaa aatcccttaa
     2821 tcaaatctca cttaaaaatc atggtgtcat aaaaaactgt tttacagctg atacatccgg
     2881 tgaacttcta tctaaatcaa aattcatatt aattgcacta gcagtattat cagttatcct
     2941 cgccaattct tctgttttat caatattctt atcgttcaat gtctgagcta ttgcttcctt
     3001 gacttgacca agtagaccta gtataactat tgtagaaatt ataagtcctt gtcttagttt
     3061 gttgttcatt cttttttttg gattaaagaa taaaaacttc ctactaatct agttaatatt
     3121 tattttttac taaaaatcaa attaaatttt gattttcttt taatctttag ctttttctat
     3181 aaaatagttt aatttattta ttcaatctct aagtcttctg atatcacttg gtgttttttg
     3241 ttcttcaatt gtattataaa tcatagaaac taacttatac ctattatttt catttgtttt
     3301 aatattaaaa tcatccattc taaaatcagt agctgttcta tagtttactc attcaaatcg
     3361 gttaccaaaa atactgtcta aaacttcttg ttctgcaata tactcttcca tgtgtctttc
     3421 tacaaaaaat ttatttatat tctcattttt gaaactacta tccattgttc tatcaagaga
     3481 tttatcaatc tgttcttcaa tatctttttc tctatcattc tttttcaata ttcctgaaaa
     3541 atctatttgt catcacttat caactaaact atcgtatgtc ggtagtgtaa aagctaattt
     3601 ttctcttttt caatgatttg ctcaaataaa aaattctcca ttttcatctt ttgttaaaaa
     3661 atcgtccatc tcaactgttc aatcatccat attataataa attcatatat tttttcattt
     3721 aatgcttcat ctaatataca atttattctt ttcatcaaca gtaatatcat cgtttatatt
     3781 taattttata tcttttataa aattttcttg attgatacta ttgatatcaa aatatgtcgt
     3841 caatccccta aaacaaccta tcatcgccct ttcatggatt tttcttaata tatctttttt
     3901 cttttcttct tcatattgtg caatatattc tttaattgca gtaagaagag tatcatctga
     3961 attttttagt gttcaatcat cattaacaaa atctttatat ttatcaaaaa gatctttatt
     4021 tctaagaaaa acttctttga attttttaac tgcatactta ggtaaccttg ataaatcttt
     4081 ttcattgtcc aaacttagac tgacttttcc actacttgag ttttctaatc ttttagctac
     4141 aaaccctttt gttcttttat tctctaaaat agcatcagaa ttagcaatat tttccaaaga
     4201 tgttggacga actaccaatt ttttttcaac agttttaggt ctttctgaat ttatgggaat
     4261 agcaatcgcg atttctatgc ctaactcttt agtaaaattt ttaaatgttt ccataaactt
     4321 ttcttcattt tcagttaata tttctcattg atttcttttt tcttgaattt tattataact
     4381 gaataaatat tgagcaaact cagtcttagt tatgctttct gtgtttaatg ttatattacc
     4441 aagatcaggc aattcttgag tgtctgttcg ttttaattct ggcaacaatc aagagtataa
     4501 agttttaagt cacccaacta aagtattaat tttttctaat ttatctttat taccttgttc
     4561 tttatcttta ttaccttgtt ctttatcttt atttatactt acctcaccag tttttatctg
     4621 ttttgctcta tttcgttctt caattaataa tctgttagtt atttcattct ctaccatggc
     4681 aactacaagc tcttcatcat attcgaaaat atctctctca ttatttcgca aattatggaa
     4741 aacatcattt caaatatgtg atatatctat tcataatcca gaaaatctag tttgatttga
     4801 aacagttttt attatatcgt ttgtaatctt gtaatatttc aaaaattttt gatcgttgtc
     4861 ttcaattttt tttcatttta cagaatcaac ctcatcaata ttattatcta taaaatctgt
     4921 gtgtgctata taattatcat ttatttcaaa catttatatt cataaaatat taaaacattt
     4981 atattttata ttttaattat aatcaaaaaa aaatgtttgt caaaaaacag ataagatatt
     5041 ttttataaca aactatttat tatttgtttg atactgttta aacgcttcct ccatattttt
     5101 taacgcatct aataatacat ctaattcttt tcatttttga tctaattctt ttcatttttg
     5161 atctaattct tttcatttta ttctttcttc aataatttct ctatctaatt ttgcaatatc
     5221 tacctccaac tccacttgtt ttattatttg agataaccta aactgatata ctctattttg
     5281 gcttatttct ttaaaagcat attgtcatag ttttgttgtt tgagtctgat ctaaaaatgc
     5341 agaaacaaat atcttttttt gttcatctgt atatcatttt caattaaaaa acttcaatat
     5401 ttgtttttca taaaaatctc gtacattgct ctgcatagct ttattccata acatatcaaa
     5461 ctctactttc tcctcttctg ttagaatatc tcatcccata aaaaactgtt ttacagccga
     5521 tgcatccggt gaacttctat ctaaatcaaa attcatatta attgcactag cagtattatc
     5581 agttatcctc gccaattctt ctgttttatc aatattctta tcgttcaatg tctgagctat
     5641 tgcttccttg acttgaccaa gtagacctag tataactatt gtagaaatta taagtccttg
     5701 tcttagtttg ttgttcattc tttttttgga ttaaagaata aaaacctttc atcatctaga
     5761 tgatactcat ttccttttaa aggtcaaatc aattttaagt tctaccattt tttgaaactt
     5821 ggacaaattt gtttaacatt tatatgagag aaacctttat gttggattca gtccattatc
     5881 gcttttttca atcaaggcaa atctctatca ttagcagaaa tatttaattt acaaccggca
     5941 gactctgtta atttcaatgg atcaaatgga gatatagtat ttcagtctgg ggtagatttt
     6001 gtttttatat tttctggtgt tgttggagaa gcttgacctg tagtcaaagc ataattttca
     6061 ttatcagcaa ctatatgcac aatattgata tcctttctag cagaatgaag cagatgccca
     6121 agtccaattc aataagtatc tccatctcat gaaactgaaa tcacagtcaa atcagggttg
     6181 gctagcttga ctccagtagc aaaagggatc cctcttccgt gaagagtttc cgctcagtat
     6241 caatccacat actgactcat tttcccacta catcaaattc atgtaactac tacagttttg
     6301 tgttttggta tttttaattc cgctaaagct tgtttgatcg caagatggat aatgaaatta
     6361 ccacatcatg gacatcgttg gaattttgta agtcctgttc tcataataac aattaataat
     6421 taaaaatttc atacatattt tctttatttt tgaataacta tctgtcatgt gttacaactt
     6481 ttccatactc tcctgttttc ttaaatcacc catcccctct tatattttaa atcatcccca
     6541 accccctact ttctgtttta aatcacccct atcccctctt tgaaaaagag gggtttccct
     6601 agcttcctcc tacgatttcc ccctcttttt caaagagggg gattaagggg gtgatttttg
     6661 ttcaaagagt gggtttaagg gggttattta tattaaaaag gagagaacaa agaggtggtt
     6721 aataaccatc tagtccgtta
//
LOCUS       DAOSID010000030         6685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_10390_length_6685_cov_12.271644, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000030 DAOSID010000000
VERSION     DAOSID010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6685
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10390_length_6685_cov_12.271644"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1730
                     /locus_tag="PK674_02030"
     CDS             <1..1730
                     /locus_tag="PK674_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15343.1"
                     /translation="EDVGRYVGYGWKEFTDKRTLSYGLLRLIYPKNNTCYGANSSARF
                     LCKNKDYINAGNIEKLEEILKSGKIKVGSQAEELIIKTLEEYQENSGSLRTNIVNLKQ
                     YYENHSIYTQKGKIYVPYRYIVPGNITFNRSLQNLSSYYTDIGFIWMFIFVFTAFGLI
                     YSIIRKNQKLFALNFATTTGWAIWRVIGGGILWYGIGLVMWTILVLSMFIQDLIINNK
                     NSENDENHGLLYTLTLGFLGLWMMVQMFFNLIRISSQGASGPFLWFQGSVGKGMEITD
                     NLRIEEKIKIGYSQKDVFDLQFPQYNKFIEYTKDRDDKDGVLIAGTYLQYFLNNPKNI
                     RFDGGLSWFWEQGSDENTCKMYQRLRSENLKYLVIDPNIATVVMGEGNESLFHRFFAK
                     IDSINGKIMEKGSILMLTQLVDEGYGKLFYSNNLGAKYGLNLSDEELSSFVGARSKDE
                     LIYLRAQLIAARFLANSDDLVNIIAQIFNTRISNGQAIGDIADVYGKIVNETKIINAM
                     NVFLSNPENVSAMVASLTQDERFVLVQYINLYSTLKQNNIQQYQEMINSIMITSLAGG
                     SQLIIVELR"
     gene            2145..2705
                     /locus_tag="PK674_02035"
     CDS             2145..2705
                     /locus_tag="PK674_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15344.1"
                     /translation="MSHIGKLLQSKKTVFTYQNLQSILLIQNRETLKSFLDRQIKAGI
                     LIRPYMGIYAFKNFDIFEFASKIKTQSYISFETVLKKEGVIFQDYGKTIFLASNNTIE
                     KKAFGYTFKCLKLKDSILYNPIGLINKGGYMIASKERAICDRLYLSQNYYFDNLEGID
                     KQKLIDISQIYNQRVILQVKKLINHA"
     gene            2698..3363
                     /locus_tag="PK674_02040"
     CDS             2698..3363
                     /locus_tag="PK674_02040"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOG15345.1"
                     /translation="MLNRDKHRVLLMKIIKDIFTSKYASQLAFKGGTASYFLYGLDRF
                     STDLDFDFFGKTSIDTGIAELLKPYGKVKIGPKMVLSYGDQETNIKIDISRKIWKANK
                     YDIVDFYGTDIKVQSKATLFTNKLVALSERFTNRDIYDVYFFFTNLFDIDEDIVYERT
                     GQSLKTLLQKIIEQLEGLPKNYKILDGLGELLDEKQKTFMKNKLISELIGIIKMKLDF
                     CEE"
     gene            complement(3433..3726)
                     /locus_tag="PK674_02045"
     CDS             complement(3433..3726)
                     /locus_tag="PK674_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15346.1"
                     /translation="MNFFQNSHPKSLIMPVFLSEKNESRNYVHLRTRKAYRSLNHNLP
                     YLYTFEDYQGEIQIPKTTNSLESVFGHMKQKIGLHRGLKKERKLKLIDDFLSK"
     gene            complement(4200..5090)
                     /locus_tag="PK674_02050"
     CDS             complement(4200..5090)
                     /locus_tag="PK674_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HOG15347.1"
                     /translation="MKKLYLIDGSGYLYRAYYAFPEMPDRDGHNINVVYGFFRMILKL
                     FHDKPDYFVIARDDPTKTHRHEIYPEYKANRVKAPDDFKAQIPIVQELVNKLNIPNLI
                     IPGYEADDIIASIVKKHNEDSEILFNIFSADKDLKQLLNDNVIFTDPSKGTPYKTKDF
                     IQEFLFQPENILDYLALIGDSADNIKGVPGIGPKGAGTLIQKYQNIENIYQHIDEISG
                     GIKEKLLDGKEEAFKSKDLISLLDVPEVNTIGIEDYRFDIDFNKYNEILVKEQNFVSM
                     EKAIKELKNKFQMPQQTSLF"
     gene            5590..5991
                     /gene="tsaE"
                     /locus_tag="PK674_02055"
     CDS             5590..5991
                     /gene="tsaE"
                     /locus_tag="PK674_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOG15348.1"
                     /translation="MELIINTPKEMFELGEKMAKDHKIILLKGDLGAGKTLFTKGFAK
                     GLEIDENSVQSPTYTYLNSYDNKLLHIDMYRLEDENEVWEKGINDQISQYDYITIERP
                     KFIELLDIYEYTVVNIEKKGETRIVRIENQQ"
     gene            complement(6000..>6685)
                     /locus_tag="PK674_02060"
     CDS             complement(6000..>6685)
                     /locus_tag="PK674_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=25
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOG15349.1"
                     /translation="DIVIGKDYIITTTGLFSEKLDMVFNQISHESKDIKNSYKSSPYF
                     ILYKIIDVFYDKTLNSLDKSAKKLLNMELNIGKNERDFVNELINEDLNKIFVKHNFIS
                     QEDVIDELVEHLNVIHEKRLIIYFNNLKQKLTKIIRTIESLEEKNDSLISAYNTYLSI
                     KSNKSITRLTLINSTFLPLTILVGMLGMSERTMMTGQTNRKIAYPLFIFLCLIIAYIT
                     YIILKKIFS"
BASE COUNT         2398 a          886 c         1060 g         2341 t
ORIGIN      
        1 atgaggatgt cggtagatat gtatgatatg gttggaaaga atttactgat aaaaggacat
       61 tatcctactg actattaaga ctcatttacc caaagaacaa cacttgttat ggagcaaact
      121 cctctgctag atttttatgt aaaaacaaag actatataaa tgctggtaat atagaaaaac
      181 tggaagagat tttaaaatct ggaaaaataa aagtttgatc acaagcagaa gaattaataa
      241 taaaaacttt ggaagaatac caagaaaact cgggttcttt gagaacaaat atagtaaatt
      301 taaaacaata ttatgaaaat catagtatct atacgcaaaa gtgaaaaata tatgttccat
      361 atagatacat tgttccgtga aatataacat tcaaccgatc attacaaaat ttaagtagtt
      421 attatacgga tattggattt atttggatgt ttatatttgt ttttactgca ttcggattga
      481 tctattctat tataaggaaa aatcaaaaac tatttgctct aaattttgct actactactg
      541 gatgggccat ttggcgagta attggatgag gaattctttg gtatggaatt ggtttagtaa
      601 tgtggacaat attagttctt agtatgttta tccaagattt gataataaat aataaaaata
      661 gtgaaaatga tgaaaatcat ggattactgt atactttaac ccttggattt ttaggacttt
      721 ggatgatggt tcaaatgttt tttaacctaa tcagaatatc tagtcaatga gcaagttgac
      781 catttttatg gttccaggga agtgtaggta aagggatgga aataacggat aatttaagaa
      841 tagaagaaaa aataaaaata tgatattcac aaaaagatgt atttgattta caatttcctc
      901 agtacaataa atttatagag tatacaaaag acagagacga caaagatggt gttttgattg
      961 ctggtaccta tttgcaatac tttttgaata acccaaaaaa tataagattt gatggtggat
     1021 tatcttggtt ttgggaacaa tgatcagatg aaaatacttg taaaatgtat caaagactca
     1081 gatctgaaaa cctgaagtat ttagtaatag atccgaatat agcaacagta gttatgtgag
     1141 aaggaaatga gtccttgttc cacagattct ttgcaaaaat tgattctatc aatggaaaaa
     1201 ttatggaaaa aggttctata ctaatgttga ctcaattagt agatgaatga tacggaaaac
     1261 tattttattc taacaaccta tgagcaaaat atggacttaa tttgagcgat gaagagttaa
     1321 gttcctttgt gtgagcaaga tcaaaagatg aattgatata tcttagagcc caattaatag
     1381 cggcaagatt tttagcaaat tctgatgatt tggtaaatat aattgcacaa atatttaata
     1441 ctaggatttc aaatgggcaa gcaatatgag atatagctga tgtatatggg aaaatagtaa
     1501 atgaaacaaa aattatcaat gctatgaatg tgtttttatc aaatccagaa aatgtgtcag
     1561 ctatggtagc aagtctaact caagatgaaa gatttgtttt ggtacaatat ataaatcttt
     1621 attcaacatt aaaacaaaac aatattcaac aatatcaaga aatgatcaat agtattatga
     1681 taactagttt ggctggtggt agtcaattga ttatagttga actgaggtag gacttaggaa
     1741 ttgaattccg ttcgcgatga gattataaat acctgccccg caaaccccgc aatactgaaa
     1801 agataaggga gaacaaaaag tttaaagtaa aatccccgcg tggctataat caatactgtt
     1861 ctggtcgaaa aaacgatagc cgaaatggga taacagacag gaacgagacg aaccagaata
     1921 gttagaaagc cgataccatc gtttacatcc tgtgcataag gaccaaaatg caatcttttt
     1981 atcttcatct catgagtttc cactttgggt atttattttc tcaaatgatg ttttcacact
     2041 agtttttaaa taaatccttg gtcagtattt tttcaaaaaa cgcattgatt tttcaaaaca
     2101 tatcattata ataggggtgt ttataaacct attccacaga caaaatgagt cacatatgaa
     2161 agctcctgca atccaaaaag acagtcttta cctatcaaaa cctacagtct attcttttaa
     2221 ttcaaaatag agagactttg aaatcttttt tggatagaca aatcaaagct tgaattctta
     2281 tcagacctta tatgtgaatt tatgctttca aaaattttga tatttttgaa tttgcatcaa
     2341 aaataaagac acaaagctac atctcatttg agacagtttt gaaaaaagag ggagtaattt
     2401 ttcaagacta tgggaaaact atttttttgg caagcaataa tacgattgaa aagaaagctt
     2461 tttgatatac ttttaaatgt ttgaaattga aagacagtat tttgtacaat cctatttgat
     2521 tgataaataa atgatgatac atgatcgcta gcaaagaaag agctatttgc gatagattgt
     2581 atttgtcgca aaattattat tttgacaatc tagaatgaat agacaagcaa aaattaatag
     2641 atatttctca aatatacaat caaagagtta ttttacaagt taaaaaatta atcaatcatg
     2701 cttaatagag acaaacacag agttttactc atgaaaatca tcaaagacat ttttactagt
     2761 aagtacgcct cacaattagc gtttaagtga tgaacagctt cttatttttt gtatggactg
     2821 gataggtttt ctaccgattt ggattttgat tttttttgaa aaacgagtat tgatacttga
     2881 attgcagagc ttttgaaacc ctatggaaaa gtaaaaatct gaccaaaaat ggttttgtcc
     2941 tatggagacc aagaaacaaa cattaagata gatattagta gaaaaatctg gaaagctaac
     3001 aagtacgata tcgtcgattt ttatgggaca gacataaaag tacaaagcaa agcgacttta
     3061 tttaccaaca aattggtggc attatctgaa agatttacaa acagggatat ctatgatgtg
     3121 tacttttttt tcacaaactt atttgatatt gatgaagata ttgtctatga aaggacaggg
     3181 caaagtttga aaacattatt acaaaagata atagaacaac tggaatgatt gccaaaaaat
     3241 tataaaatac tggacggatt gggagagtta ctggatgaga aacaaaaaac ttttatgaaa
     3301 aacaaactca tatctgagct gatagggatt atcaaaatga aacttgattt ctgtgaggag
     3361 taagaaaagt tttggaaatt ggtataagga gaacattttg aaattaatgg cataattaaa
     3421 gattttgggt ggctatttag agagaaaatc atctattaat ttaagttttc tttctttttt
     3481 taatcatcta tgtaatcata ttttctgttt catatgtcaa aacactgatt ctaatgaatt
     3541 tgttgtctta ggaatttgta tttctcattg gtaatcttca aatgtataca aatatggtaa
     3601 attatgattt aagcttctat atgcttttct tgtcctgaga tgaacataat ttctactctc
     3661 atttttctct gataaaaata ctggcataat taaagatttt gggtggctat tttgaaagaa
     3721 attcatctat taacttaagt tttctatgtt tttttaatca tctatgtaat catacttttt
     3781 gtttcatatg tgaaaacacc gattctaggg aatttgttat tttaggaata tctatttctc
     3841 attcatattt ttcaaatgta tataaatata ataaattatt ttttaaactt ttgtaagctt
     3901 ttctggtcct tatacctttg ttttcctctt ttaaatcaat cttttttgat cctatttgat
     3961 caacattttt tacaaatttt ataatacatt tataaaaaaa cagtagataa agtaattaac
     4021 ttctactgtt tcctatcatt ttagccaccc gtttttttta attatgccat tattatctac
     4081 ctttttgaac aaagtgaaat tctctctcgg tatatttcaa gcaaagagag attcccaatg
     4141 aagaggttca aatctcatgt cgaagcaggc tttagaaaac ttaaagactt tccaacttac
     4201 tagaacaaac tagtttgttg tggcatttga aatttatttt tcaactcctt aattgctttt
     4261 tccatagata cgaaattttg ctcctttacc aaaatttcat tatatttatt aaaatctata
     4321 tcaaatctat aatcttctat accaatagta ttcacctcag gaacatctag tagagaaatt
     4381 aagtcttttg acttgaatgc ctcctctttt caatctagta acttttcttt gattcaacca
     4441 cttatttcgt ctatatgttg gtatatattt tcaatgtttt gatacttttg tatcaatgta
     4501 ccagctcact ttggtcatat cccgggaact catttaatat tatcggcact atctccaatt
     4561 aaagccaaat aatccaaaat attttctggt tgaaataaaa actcttggat aaaatccttt
     4621 gttttgtacg gtgttcattt gcttggatct gtaaaaatca cattatcatt tagtaattgt
     4681 ttcaagtctt tatcagcaga aaaaatatta aaaagaattt ctgaatcttc attatgtttt
     4741 ttaactattg aagcaataat atcatcagcc tcatatcaag gaattattaa attgggaata
     4801 tttagtttgt ttactaattc ttgaacaatc ggaatttgtg ctttgaagtc atctggtgct
     4861 tttactctat tagctttata ttcaggataa atttcatgtc tatgtgtttt tgtaggatca
     4921 tctcgtgcta ttacaaaata atctggttta tcgtgaaata atttcaaaat catacgaaaa
     4981 aatccataaa ctacatttat attgtgacca tcacgatcag gcatttcagg aaaagcataa
     5041 tatgctctat ataaatatca tgatccgtct attaagtaca attttttcat tatttgagat
     5101 tttattcata aaaattattt gtttttttcc aggtttgaag ggttggtagt ccctcccttg
     5161 ggggagggtg ttcccccgca gtattgcggg gtagttgggg gttgtggctt tagatccccc
     5221 ttaatccccc ctctttgatt ctaaatcacc cccttaatcc ccctctttcc cgtagctagt
     5281 acgggacact gcgaagaaga ggggggaatc tcgtattgag cccccttctt ttttcgactg
     5341 aaaggagtcc tctcccgata gatcgggatc tcactttgtt cgagatttat cggggaaaga
     5401 agggggttgg gggatgattt aaagtataag agggtttgga gtgatttaag aagggtgtga
     5461 gggaacttag aatataaaat ggtgataaca aatatgtcac taacttaaat ctatctttta
     5521 ttgaattaaa agcaataaaa agtatattat tcaagcaaac aatcattttt ttaactatac
     5581 taaaaaaaaa tggaattgat aataaatact ccaaaagaga tgtttgaatt atgagaaaag
     5641 atggcaaaag accataaaat aattttacta aaatgagatt tgtgagctgg taaaactcta
     5701 tttacaaaag gttttgctaa atgattggag atagacgaaa atagcgttca aagccccact
     5761 tatacttatc tgaattcata tgataacaaa ttactacata tagatatgta cagattagaa
     5821 gatgaaaatg aagtttggga aaagggaata aatgaccaaa tttctcaata tgattatatt
     5881 acaatagaac gaccaaaatt tattgaatta ttggatattt acgagtatac agtagtaaat
     5941 attgaaaaaa aatgagaaac aagaatagtg cgtattgaaa atcaacaata agttaaattt
     6001 tatgaaaata tttttttaag tataatatat gtaatatatg ctataattaa acacaagaaa
     6061 ataaataaag gataagctat tttccgattt gtttgacctg tcatcatggt tcgttctgac
     6121 atccccaaca ttcctacaag aatggtaaga ggtaaaaatg ttgaattaat aagtgttagc
     6181 cttgtaatac ttttattgct ttttattgat aaatatgtgt tatatgcaga tataagagag
     6241 tcattttttt cttctaaact ctctattgtt ctaataattt ttgtaagttt ttgttttagg
     6301 ttattaaaat atataataag gcgtttttca tgaattacat tcaaatgttc taccaattca
     6361 tcaataacat cttcttgcga aataaaatta tgttttacaa aaattttgtt taaatcctcg
     6421 tttattaatt cgtttacaaa atctcgttca ttttttcaaa tatttaactc catattcaga
     6481 agtttttttg ctgatttatc taaggaatta agtgttttat cataaaaaac atcaataatt
     6541 ttataaagaa taaaatatgg agatgatttg tatgagtttt taatgtcttt tgattcatga
     6601 gatatttgat tgaataccat atcaagtttt tctgaaaaca atcatgtagt ggtaataata
     6661 taatcttttc aaataactat atcca
//
LOCUS       DAOSID010000031         6630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_10508_length_6630_cov_14.258707, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000031 DAOSID010000000
VERSION     DAOSID010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10508_length_6630_cov_14.258707"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..616)
                     /gene="rpsI"
                     /locus_tag="PK674_02065"
     CDS             complement(194..616)
                     /gene="rpsI"
                     /locus_tag="PK674_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="30S ribosomal protein S9"
                     /protein_id="HOG15350.1"
                     /translation="MVANEYNYAVGRRKETSAVIKLFPKGKGDFIIRLSSGKTLPLKE
                     YFGGNAHLYENAIMPLLILGKDYIRQFDAEIVIRGGGVAGQSDAIKLAFARSLVDRNS
                     DFRTQLKPYGLLKRDSRIKERKKPGLKKARKAPTWSKR"
     gene            complement(616..1077)
                     /gene="rplM"
                     /locus_tag="PK674_02070"
     CDS             complement(616..1077)
                     /gene="rplM"
                     /locus_tag="PK674_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L13"
                     /protein_id="HOG15351.1"
                     /translation="MEFTKKDLNKTLWVKRENQEKNRKRYKVDATGLILGRLAVDIAN
                     KLAGKDKSYLSGFWDAGSYVIVENAGKFIVKGNNKLKQKKYYTYSGYKGNLRSISLDE
                     LIQKNPTKALWYAVRGMLPKNKLRDSRMKRLKLVVDTTTKYDNFKPVNLYK"
     gene            complement(1136..1525)
                     /locus_tag="PK674_02075"
     CDS             complement(1136..1525)
                     /locus_tag="PK674_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="L17 family ribosomal protein"
                     /protein_id="HOG15352.1"
                     /translation="MRHRINKLKQLNTGAKKKNVFVRNLLTSFVKTGRITTTPKRAKV
                     LKAETDAFFSKLVKTYSRYNTEAESKRECIKIVKATIFGEAEGKKVILEWLPKYLGGD
                     KRTSYVMSYKLGVRKGDSAEKIMLKLV"
     gene            complement(1552..2547)
                     /locus_tag="PK674_02080"
     CDS             complement(1552..2547)
                     /locus_tag="PK674_02080"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HOG15353.1"
                     /translation="MLDIQKFIGTPKIIVSEEENNVTKFELNYLPRGFGNTLGNALRR
                     IILSYNIGGAITGLKIKGVPHEYHVIDGVKEDVIAIMLNFKKLRFKFDPSVESIQWVS
                     QRFKGIGQYSSDFLKLPTGIELLNTNEYLFEITDPSLELNIDLRIEKGYKYYSVEFLR
                     SRDSEQENVDVNTLLIDNEFKLVDYIKYDVDEVIDDFTGGTKDSLKLEIRSLFAGVSA
                     KEIVMFAGEVLASYAKLFIFDDVYIDRSVLLEYNELNFEKELLPEEMNIKTMPIDALP
                     LSERTRNALIKNSVLYVEDLEKKKKGELLLMKGVGRKAIDEITQSLANIGKSLAG"
     gene            complement(2623..3243)
                     /gene="rpsD"
                     /locus_tag="PK674_02085"
     CDS             complement(2623..3243)
                     /gene="rpsD"
                     /locus_tag="PK674_02085"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="30S ribosomal protein S4"
                     /protein_id="HOG15354.1"
                     /translation="MKYTGPKFKLCRREQANLFGSDKYDIKKRRSLPGQHGSSMKRHS
                     EYGKLLRNKQILKRSYLLSEKQFRNLVQKQSSKYSKNNGVSHDLALYIFLESRIDSII
                     YRSGLAKTIIQARQAVNHGHFLLNGRKHNIPSTFIKIGDKITLKTKLKDSPLYTGITV
                     SKTQKIPSWIKVDRNKYEVEMLSLPKLGELGVLADILKVIEFYARA"
     gene            complement(3284..3673)
                     /gene="rpsK"
                     /locus_tag="PK674_02090"
     CDS             complement(3284..3673)
                     /gene="rpsK"
                     /locus_tag="PK674_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="30S ribosomal protein S11"
                     /protein_id="HOG15355.1"
                     /translation="MASIKKTNKKKDIKITSGVLHVKTTPNNTLIILTDNEGNKILGG
                     GTGLLGYKGAKKDTPYAAEVLTKNILKEAQSFGLKEIGLVIKGIGMARDGVFKAINEV
                     ALVDLLYIQEATPIQFGGVKGVRPKRN"
     gene            complement(3704..4078)
                     /gene="rpsM"
                     /locus_tag="PK674_02095"
     CDS             complement(3704..4078)
                     /gene="rpsM"
                     /locus_tag="PK674_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="30S ribosomal protein S13"
                     /protein_id="HOG15356.1"
                     /translation="MFRLMGHVLPEDKSIWIAMTSIYGIGRSRSKKILDKLGIPFMKK
                     VKDISEEEQKKISDELQNYVLESDLKREIASAIKRLKEIKCYRGMRHSIGLPVRGQVT
                     RKNARTAKKLLGRSKVRPILKK"
     gene            complement(4126..4242)
                     /gene="rpmJ"
                     /locus_tag="PK674_02100"
     CDS             complement(4126..4242)
                     /gene="rpmJ"
                     /locus_tag="PK674_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L36"
                     /protein_id="HOG15357.1"
                     /translation="MKVKSSIQKRCMFCQIVKRKGKLYVICKKNPRHKTRQG"
     gene            complement(4276..4557)
                     /gene="infA"
                     /locus_tag="PK674_02105"
     CDS             complement(4276..4557)
                     /gene="infA"
                     /locus_tag="PK674_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="translation initiation factor IF-1"
                     /protein_id="HOG15358.1"
                     /translation="MVKEVALERDGEVLEKLPAGILKVKLKDVGAEIRCKLSGKMKQK
                     HISVIPGDWVKVEVNQYDMTQGRIVYRYNSYDSTQNDVETTELGDMETE"
     gene            complement(4550..4873)
                     /gene="rplU"
                     /locus_tag="PK674_02110"
     CDS             complement(4550..4873)
                     /gene="rplU"
                     /locus_tag="PK674_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L21"
                     /protein_id="HOG15359.1"
                     /translation="MYAVIEVKGHQYIVKKGDTIVVDKIEGNKGDKINIEEVLLAFDD
                     KGEKVIVGSPYIQKAKVSAELLETKKGKKIGVLKFKRKNRYQRRIGFRPCETVLEIKK
                     IEIDG"
     gene            complement(4907..5170)
                     /locus_tag="PK674_02115"
     CDS             complement(4907..5170)
                     /locus_tag="PK674_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15360.1"
                     /translation="MGFTVRRTGDLTTNERMISFYKKELRSSHILEKAKKQKHYGTKR
                     YRKFVVYDFPSSRRYKRVKAIVSNAYRTNKKKYNTLAQLGKIA"
     gene            complement(5212..5297)
                     /locus_tag="PK674_02120"
     tRNA            complement(5212..5297)
                     /locus_tag="PK674_02120"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5261..5263),aa:Leu,seq:taa)
     gene            complement(5339..5686)
                     /gene="rplT"
                     /locus_tag="PK674_02125"
     CDS             complement(5339..5686)
                     /gene="rplT"
                     /locus_tag="PK674_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L20"
                     /protein_id="HOG15361.1"
                     /translation="MVRVSSGLQRQRSHKKYIKAAKGFRLGRKNLYTQVKDALVKQGQ
                     HAYVSRKLKKRDFRRLWTGRLSASLREKGSKYSAFIGQMSKKNILLNRKVLSNIAVAF
                     PEVFDKIYKEVVK"
     gene            complement(5695..5892)
                     /locus_tag="PK674_02130"
     CDS             complement(5695..5892)
                     /locus_tag="PK674_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L35"
                     /protein_id="HOG15362.1"
                     /translation="MANKAKSHSGASKRFKITKTGKVLTSKSCNNHLLTNKGKTNKKS
                     PYGKKLSKANSRMVKILLPYK"
     gene            complement(5901..6446)
                     /gene="infC"
                     /locus_tag="PK674_02135"
     CDS             complement(5901..6446)
                     /gene="infC"
                     /locus_tag="PK674_02135"
                     /inference="COORDINATES: protein motif:HMM:NF017049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="translation initiation factor IF-3"
                     /protein_id="HOG15363.1"
                     /translation="MQERPFEHKGERIVYVNSSIKAGTIVIVDEKKNNLGSFPRRVAL
                     EMAEQKGLDLVQIHYDPVKQVCTALLTDYGKYMYRKQKDEREKKKTQKQKVLKELKIN
                     YSIGNNDLELKIKKGREFLLEGNNVKFSIRLKGRERMYESKAVDMLNQIVEKLSDAGK
                     TQYSTPKKEAQGYSIILFSKF"
     gene            complement(6483..6557)
                     /locus_tag="PK674_02140"
     tRNA            complement(6483..6557)
                     /locus_tag="PK674_02140"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6520..6522),aa:Glu,seq:ctc)
BASE COUNT         1959 a         1202 c          816 g         2653 t
ORIGIN      
        1 agaaaaaaat aaggcaaaaa aaaaccctcg attgaaaaat cgagggctat tttatataaa
       61 gaattgcttg gataaggaga gattgcttag atagggaaga attgcttaga tgaaggtatt
      121 aaaatttatt ttattacaaa aaaaaactca aaataaattg agtctttttt tggtatatca
      181 atatcgtatt tttttatctt tttgaccaag ttggagcttt tctcgctttt ttcaatcatg
      241 gcttttttct ttctttgatc ctagaatctc ttttgagtaa gccataaggt tttagttggg
      301 tcctaaaatc actatttcgg tctaccaaag acctagcaaa tgctaatttg atagcatcag
      361 attgtccagc cacacctcat catctaataa cgatttctgc atcaaattgc ctaatgtaat
      421 cttttcataa tatcaacaac ggcataatag cattttcgta taaatgtgcg ttacctccaa
      481 aatattcttt tagaggaagt gtctttcaac tagataatct gataataaag tctcatttcc
      541 cttttggaaa taatttaatt acggctgaag tctcttttct tctacctaca gcataattgt
      601 attcgtttgc aaccattatt tatataaatt tacaggttta aaattatcat attttgtagt
      661 tgtgtctact actaatttaa gtcttttcat cctagaatct ctaagcttgt ttttgggtaa
      721 catccccctc acagcatacc ataaagcttt tgttggattt ttttggatta gctcatccaa
      781 agaaatactt ctgagatttc atttgtaacc actataagtg taatactttt tttgtttgag
      841 cttgttgttt cattttacta taaattttca agcattttcc actattacat aagatcatgc
      901 atcccaaaaa cccgacaaat acgatttgtc tttgccagca agtttgtttg ctatatctac
      961 ggctagtctt cataaaatta gaccagtagc atctactttg tatcgttttc tgtttttctc
     1021 ctgattttct ctctttaccc aaagagtttt attcagatct ttttttgtaa attccatctt
     1081 taaaccgatt tctatatata aaacactaaa atactatttt ggtatttaaa ctatattaaa
     1141 ccaatttcag cattattttt tcagctgaat ctcattttct aactccgagc ttgtaagaca
     1201 tcacataaga ggtcctttta tctcctcaaa gatatttggg caaccattcc aaaataactt
     1261 ttttgccttc agcttcccca aaaatagtag ccttcactat ttttatgcac tctctttttg
     1321 attcagcctc agtattgtat ctggaatatg tttttacaag tttagaaaaa aatgcgtcag
     1381 tttccgcttt tagaactttc gctcttttgg gagttgttgt aattctacca gtttttacaa
     1441 aactggtcag taagtttctt acaaaaacat tttttttctt agcacccgta ttgagttgct
     1501 ttaatttatt gattctatgt ctcattttga tcaaacaaat aatttaaatt tttatccagc
     1561 tagtgatttc ccaatatttg ccaaagactg ggttatttca tcaatagctt ttcttccaac
     1621 acctttcata agcaacaatt ctcatttttt ctttttttcc aaatcttcta catacaaaac
     1681 agaatttttt attaaggcat ttcttgtcct ctcactcaaa ggtaatgcat caattggcat
     1741 tgtttttata ttcatttctt ctggtaacag ctccttttca aaattaagtt cgttgtattc
     1801 taataacaca gacctatcta tatatacatc atcaaagata aataatttcg cataagaagc
     1861 tagcacttct ccagcaaaca ttactatttc ttttgctgat actccagcaa ataatgatct
     1921 gatctccaat ttcaaactgt cttttgttca tccagtaaaa tcatctatta cttcatctac
     1981 atcatatttt atgtaatcaa ctagtttaaa ctcgttatct ataagcaagg tatttacatc
     2041 cacattttct tgttcactat cacgacttct taggaattca acactataat atttatatca
     2101 cttttctatt ctgagatcta tattcaactc caaagacgga tctgtaattt caaacaaata
     2161 ctcgtttgta tttaatagtt caattcaagt aggtaatttc aggaagtcag aactatattg
     2221 tcagataccc ttgaatcttt gtgaaaccca ttgtattgat tccacactag gatcaaattt
     2281 gaacctaagc tttttgaaat ttaacataat cgcaatcaca tcttctttta ctcagtctat
     2341 tacatgatat tcgtgtggaa ctcatttgat tttcaaacca gtaatcgctc atcaaatatt
     2401 ataagataaa ataattctcc tcagagcgtt tcacagagtg tttccaaatc atctaggtaa
     2461 ataatttaac tcaaattttg ttacattgtt ttcttcttcg gaaacaatga tttttggcgt
     2521 acctataaac ttttgaatat ctaacatagt gtattaaaaa gaatataaat ttttatttta
     2581 gaatatattg taatattgta ataaaggggg gttggggaat aactatgctc tcgcatagaa
     2641 ttcaattact tttagtatat cagccaaaac tcacaactct cacagtttag gcaaggagag
     2701 catctccact tcatacttgt ttctatctac cttgatccat gaagggattt tttgagtctt
     2761 actgactgta atcccagtat ataacggaga atcttttagt ttggtcttta aggtaatttt
     2821 atctccaatt tttataaaag tagaaggtat attatgtttt ctaccgttta acaaaaagtg
     2881 tccatgattt actgcctgcc tagcttgaat aattgtcttt gctaatccag atctataaat
     2941 tatagaatct attcttgatt ccaaaaaaat atataaagcc aaatcgtgac taactcagtt
     3001 attcttggaa tattttgatg attgcttttg aactaaattt ctaaattgtt tttcggacaa
     3061 aagataagat cttttcaaaa tctgtttgtt tctcaaaagc tttccatatt ctgaatgtct
     3121 tttcatagaa gaaccatgtt gtcatggaag tgatcttctt tttttgatat catatttatc
     3181 acttcaaaac aaatttgctt gttcccttct acataattta aactttggtc atgtatactt
     3241 cattgataat aattaattat aaatttttga atattttgat tttttagttt ctttttggtc
     3301 taactccttt tactccacca aactggatcg gtgtagcttc ttgtatatac aacaaatcta
     3361 ccaaagctac ttcgttgata gctttgaaaa ccccatccct tgccatacct attcacttta
     3421 ttacaagtca tatctctttt aatcaaaaac tttgagcttc tttcaaaata ttttttgtga
     3481 gtacttctgc cgcataggga gtatcttttt ttgctcattt atatcacaaa agtcatgtcc
     3541 ctcctcctag aatcttgttt cattcattat ctgtcaaaat gataagagta ttgtttgggg
     3601 tggtttttac atgtaaaact cagctcgtaa tttttatatc ttttttcttg tttgtctttt
     3661 ttatagaagc cataattaaa ataatttaat taactaaata aaactatttt ttcaaaatag
     3721 gtctaacttt tgatctaccg agaagttttt ttgctgtcct agcatttttt ctagtaactt
     3781 gtcatctaac gggcagacca atactgtgtc tcatccctct gtaacacttg atttctttta
     3841 accttttgat agcagaagct atctctcttt tgagatcgct ctccaaaaca tagttttgta
     3901 gctcgtcact aatttttttt tgttcttctt cagaaatatc ttttactttt ttcataaaag
     3961 ggattcccag tttatctaat attttttttg atctacttct gccaataccg tatatgctag
     4021 tcatagctat ccatatagat ttgtcctcag gtagaacatg tcacatcaat ctaaacattc
     4081 tttcaaattt ttaattaaaa taaattattt atataaaaca aaaaactatc cttgtctagt
     4141 tttgtgtctt ggattttttt tgcaaattac ataaagtttt ccttttcttt ttactatctg
     4201 acagaacata catctttttt gtatagaact ctttactttc atttcgatca aataaacaat
     4261 ataaattata gcatattatt cagtctccat atctcaaagc tctgtagttt caacatcatt
     4321 ttgagttgaa tcgtagctat tgtacctgta tacaatacga ccttgcgtca tatcatattg
     4381 atttacctcc acttttaccc aatcacctgg tattacagaa atatgctttt gcttcatctt
     4441 accagatagt ttgcaacgga tttccgctca aacatctttt aacttaacct ttaggattca
     4501 tgctggaagt ttttcaagca cttctccatc tcgctctaaa gcaacttctt taaccatcaa
     4561 tctctatttt tttaatttct aaaaccgttt cacagggcct aaatcaaatc cttctttggt
     4621 atctgttttt tcttttgaac ttcaaaacac ctattttttt tcatttttta gtttccaaaa
     4681 gttctgcaga tacttttgcc ttttgaatat aaggagatca aactattacc ttttctcatt
     4741 tgtcatcaaa agctaataaa acttcctcga tatttatttt atctcactta tttcattcta
     4801 ttttatctac aactatagta tctcattttt taactatata ttgatgtcat tttacttcta
     4861 ttacagcata cataatgtcg ttggtttata tatttaaaat ttgaagctaa gcaatttttc
     4921 ataattgtgc caaagtatta tattttttct tattagtcct atatgcgttt gaaacgatag
     4981 ctttcactct tttgtatctc ctagaactag gaaaatcata aacaacaaat tttctatatc
     5041 gctttgtgcc atagtgcttt tgttttttag ctttttccaa aatatgagaa gatcgtaact
     5101 cctttttata aaaagaaatc attctctcat ttgtagttaa gtctcaagtt cttcgaacag
     5161 taaatcacat gattttatta tttaataata aataaatttt tgattttaat ttgccgagaa
     5221 ccggaattga accggtactc catattactg gaaatggatt ttaagtccat cgcgtctacc
     5281 aattccgcca ccccggcttg gtttcaacac ttaatattgt aagtgtctaa acgctaatct
     5341 atttaacaac ttctttgtat atcttatcaa aaacctctgg aaaagctact gcaatatttg
     5401 acaaaacttt tctattcaaa aggatgtttt tcttggacat ttgtccaata aaagcactat
     5461 acttggatca cttttccctt aaactagccg acaaccttcc tgtccataat ctcctaaaat
     5521 ctcttttttt tagttttcta gaaacatagg catgttgtca ttgttttact aaagcatctt
     5581 ttacttgggt atacaagttt tttctaccca gtctaaaacc tttggcagct ttaatatatt
     5641 ttttgtgaga tctttgtctt tgtaacccac tagaaaccct aaccattttt tatgttattt
     5701 ataaggaagt aaaattttga ccattctgct attagctttg gataattttt ttcaataagg
     5761 acttttttta ttcgtcttac ctttgttggt aagtaggtga ttattacaag attttgaagt
     5821 taaaactttt caagtttttg taattttaaa tctttttgat gcaccactat gtgattttgc
     5881 tttatttgcc atctttttaa ttaaaattta ctgaataaaa taatactata tcattgtgcc
     5941 tctttttttg gtgtagaata ttgtgttttt caagcatcag ataacttttc tacgatttgg
     6001 tttaacatat ctacggcttt tgattcatac attctttccc ttcacttcag tcttattgag
     6061 aattttacat tatttcattc taacaaaaat tctcttcatt tttttatttt taattctaag
     6121 tcgttatttc atatagagta gttgattttc aattctttca aaactttctg tttttgagtt
     6181 ttcttttttt ctctctcatc tttttgtttt ctatacatat atttaccata atccgtcaat
     6241 aatgcggtac aaacctgttt tacggggtca taatgtattt gaactaaatc aagacctttt
     6301 tgttctgcca tttccaaagc tacccttctt gggaaagatc acaagttatt ttttttttca
     6361 tccacaataa ctattgttca agcttttata gaactgttta catatactat tctttctcat
     6421 ttatgctcaa aaggtctttc ttgcatacaa tattttataa actaaatagc caaggtctaa
     6481 ttcgctcccg gcgggactcg aacccgcgat cttcgccgtg agagggcgat gtcctaaaac
     6541 cgctagacta cgagagccca caaagtttta ttaaatttat ccccaggtgg atcccgcaat
     6601 gtgcgggaca agcccgaacc accaacttat
//
LOCUS       DAOSID010000032         6600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_10574_length_6600_cov_11.530940, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000032 DAOSID010000000
VERSION     DAOSID010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10574_length_6600_cov_11.530940"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /gene="asnS"
                     /locus_tag="PK674_02145"
     CDS             complement(<1..1373)
                     /gene="asnS"
                     /locus_tag="PK674_02145"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004244664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOG15364.1"
                     /translation="MKTTSIKTIYNDYETFIGQELLVKGRIRSLRDSKTFGFIELNDG
                     SYLKNLQIVFDSALKNFDEICKYTTGSSIEVHGTLVKSEGAKQAFELKATEVILVGKA
                     DESFPLQKKRHSFEYLRTIAHLRPRTNTFSAVFRVRSLLSFAIHDFFNKNGFIRAHTP
                     IITSSDCEGAGEMFQVTTLDLANPPKKEDGSIDFSKDFFGKHASLTVSGQLEGETFAT
                     AFGKIYTFGPTFRAENSNTTRHLSEFRMIEPEIAFADLNDNMQLAEDLLKYSFKYVLE
                     QAPAEMEFFDKFIMPGVKARLEALVNSSFGRVTYTEAIELLKKANHKFEYQPEWGIDL
                     QTEHERYLSEQIFKGPVFVTDYPKEIKAFYMKQNDDGKTVRAMDLLVPGVGELIGGSQ
                     REDDFDKLKTRMEEMQLDTEEYSWFLDLRKYGTVPHAGFGVGFERLVMYVSGMENIRD
                     VIPFPRAP"
     assembly_gap    1424..1523
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1949..3484
                     /locus_tag="PK674_02150"
     CDS             1949..3484
                     /locus_tag="PK674_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOG15365.1"
                     /translation="MKKSGYKELLPDQTLGEKLIKKGFWLYFFSFLIAPTGYIIKVII
                     SNTVSIADVGVLYSIIGLITIINVYNDLGLTESLQYFLPRYWIKKQYNYIKTSIFLSL
                     GVQMFTGLIIAGILWFGAPWLATNYFQSPNAVVILRYFCFYFLGINVFDILISIFYAF
                     QNTLAQKSIEFIRMVSVVGFTLFFFFSGRTSIERYSLNWILGLAIGIIAGLIIFFVKY
                     KKNLFQGKIVFEKNMLKEYIKYAFWCFLGLNALYLFGQIIQQLVILILGPEAAGFYTN
                     FLSLYGISAVIIGPIIGLVFPMVSELITKKDTKKLQTLFNFFYTYFSVFAFFLAIFLM
                     ALGPEVALVLFGKKFILSGKMVILGALFTIANVFVGFNFSVLAGLGKIKERVEILFTT
                     VLITVVLSVIGMYSLGVYGAVLTLGIGETILFILSFKLVYKDIKFGVNWRFIIKNICI
                     GLLLGIILWMIKSKIFVNDDSMRLFNLWRLILIGLGFFVVFGLFNFQKALVLKGEIKK
                     LRT"
     gene            3581..4147
                     /locus_tag="PK674_02155"
     CDS             3581..4147
                     /locus_tag="PK674_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15366.1"
                     /translation="MKITSLLKTKKTVFKIDDFYQILGLENKFSLRNLITRRNKKGLL
                     KNVFYGVWCLPNYNKYELANILQRPSYISLETVLYQKGVIFQNYSSTISSVGLKTRNI
                     NIGNITYSYKTIKKETFYDQLGIVYKDNYAIATVERALCDMLYLNPSYYFDNLRAINR
                     EKVFEIAKIYNNKRLILDLEKIKNGIEY"
     gene            4131..4829
                     /locus_tag="PK674_02160"
     CDS             4131..4829
                     /locus_tag="PK674_02160"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOG15367.1"
                     /translation="MALNIKKHRIIMINILKDIVGSVYGKHLGFKGGTLLYLLYNLDR
                     FSTDLDFDLLNKGSINEEKMLLDLAKICSKHGKVKDMYNKEKTLFLLVDYEPYEMKIK
                     IEINKKRSIHSKYEYKSILGYQVLCMEAESLFANKLVALTQRKNIASRDLFDVHFFLK
                     KGFEINENIIKERTGKSLKSYLKYVIDFITKNFTEKTLLAGLGELVDQKQKYFIKNLL
                     VQETCEYLKLIISD"
     gene            complement(5069..5386)
                     /locus_tag="PK674_02165"
     CDS             complement(5069..5386)
                     /locus_tag="PK674_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HOG15368.1"
                     /translation="MKTAILIHPGEILADELEARGWTQKQLAELIQKSPQEVSFIITG
                     KRNLNADWALRLESAMGISAQSRLNMQNFYDLQLQEQEKASILSGIRILAEKKTTNFA
                     IAS"
     gene            5551..6360
                     /locus_tag="PK674_02170"
     CDS             5551..6360
                     /locus_tag="PK674_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="BRO family protein"
                     /protein_id="HOG15369.1"
                     /translation="MSNLALFQEKEIRRHWDAKQEKRYFSVVDIVGVLSESKDPRNYW
                     KVLKNRLTKEGSELVTNCNQLKLVASDGKKYLTDVADVETILRLVQSIPSPNAEPFKV
                     WLAKVGYERMQETVDPALSLERARENRKKHGRSEKWIQQRMMGQETRNKLTDYRHEHG
                     IKKGDEYAILTNIIHQEWTGMTVQEHKKMKNLKTQNLRDHMSEAELIFTALAELSTRQ
                     IAETTQATGMKENRVASKKGGTIAKQARKKLEDQTGKSVISDQNFLPPKKK"
     gene            6385..>6600
                     /locus_tag="PK674_02175"
     CDS             6385..>6600
                     /locus_tag="PK674_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOG15370.1"
                     /translation="MKTVVITSGYYNPIHPGHIECIELCKELGDELWVIVNNDEQARA
                     KTGKQEVFQDENFRMRVVGAIKGVDQVF"
BASE COUNT         2316 a          936 c         1049 g         2199 t
ORIGIN      
        1 ggagctctag ggaaaggaat tacatcacgg atattttcca ttcaactgac atacatcacc
       61 aatctctcaa atcccactcc aaatccagca tgtggaacag tcccatattt tctcagatca
      121 agaaaccagc tgtattcctc agtatcaagc tgcatctctt ccattctcgt tttaagttta
      181 tcaaagtcat cttccctttg gcttccacct attaattctc atactcctgg aacaagtaaa
      241 tccattgctc ttacggtctt cccatcatca ttctgcttca tataaaaggc tttgatttct
      301 tttggataat cagttacgaa aacaggtcct ttgaatattt gttctgataa atatctttcg
      361 tgttcagtct gcaaatcaat tccccattct ggttggtatt caaatttatg attagctttt
      421 ttcaaaagtt ctattgcttc ggtataagtc actctaccaa atgatgagtt tacaagtgct
      481 tcaagtctgg cttttactca tggcatgatg aatttatcaa agaattccat ctcagctggt
      541 gcttgttcca aaacatattt gaaagaatat ttcaacaaat cttcagcaag ttgcatatta
      601 tcgtttagat ctgcgaaagc gatttctggc tcaatcattc ggaattctga aaggtgtctg
      661 gtggtgttgg aattttctgc acgaaaagtt ggcccgaatg tataaatctt tccaaaagca
      721 gtagcaaaag tctctccctc aagctgtcca cttactgtaa gtgaagcatg ctttcaaaaa
      781 aagtctttgc taaaatcaat agatcagtct tctttttttg gaggatttgc taaatcaagc
      841 gtagttactt ggaacatctc ccctgctcct tcacaatcac ttgaagtaat aataggggta
      901 tgagctcgaa tgaaaccatt tttattgaag aaatcatgaa ttgcgaaaga aagcaaactt
      961 cttaccctga aaaccgctga aaaagtattg gtccttggcc tgagatgagc aattgttcta
     1021 agatattcaa aagaatgtct ttttttctgg agtggaaagc tctcatcagc ctttcctacg
     1081 agtattactt ctgtcgcttt taattcaaaa gcctgttttg ctcactctga ttttacaagt
     1141 gttcaatgaa cttctatact acttcctgtc gtatatttgc agatctcatc aaagtttttt
     1201 aatgctgaat caaaaacaat ttgcagattt tttaggtaag aaccatcatt tagttcaata
     1261 aaaccgaaag ttttggaatc tctaaggctt ctaattcgcc cttttactaa aagctcctgt
     1321 ccaataaaag tttcgtaatc attataaata gttttaatac ttgttgtttt cataatagaa
     1381 tcaataaaaa ttaaaatcaa tttgaattaa tatatttttt ttannnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnttgtgga caaatccccc aacccccttg cttatcttaa
     1561 atcatccccc taccccccct taagaaaggg ggctgtatag gtggatttgg gaaatgacgg
     1621 ggataaaatt cctccccttg gggaggatga atgtcccgaa tttatgaggg actaggtggg
     1681 ggttttaccc ccaactacga tagaacaact tccccgttgg ggaaggattt cctacttatt
     1741 cccaacaact ttttcccacc tatggcctaa atcccccctt gcttacggag ttctccgtaa
     1801 caagtaaagg ggtactcaaa aactttacaa accccacaat actgtgaggc aaactttcca
     1861 aacttttagg tttgattttg gattaatgat tagtatataa tatatgctca caaaatagca
     1921 ttattatttt tttgaaagct aaaaacgcat gaaaaaaagc tgatataaag aactattacc
     1981 agaccaaaca ttaggagaaa aactaatcaa aaaatgattt tggttatatt ttttttcgtt
     2041 cttgatcgca cctacaggat atattatcaa agtaataatc tcaaacacgg tatcgatagc
     2101 tgatgttgga gtactatata gtatcatttg actgataaca atcataaatg tgtataatga
     2161 tctaggtctc acagaaagtt tacaatattt cctacctaga tactggataa aaaaacaata
     2221 taattatatc aaaacatcaa tatttttgag tctaggtgtt caaatgttta cctgactaat
     2281 tattgctgga atactgtggt tttgagcacc atggctggct actaattatt ttcaaagtcc
     2341 caatgctgta gtaattttaa ggtatttttg tttctatttc ttatgaataa atgtatttga
     2401 tattttaata agtatttttt atgcatttca aaatacttta gcgcaaaaat ctattgaatt
     2461 tattagaatg gtaagtgttg tttgatttac tttatttttc tttttttcag gtaggactag
     2521 catagaacgg tacagtctaa actggatatt aggcttagct atatgaataa tagctggact
     2581 aataatcttt tttgtaaaat acaaaaaaaa cctttttcaa tgaaaaatag tctttgaaaa
     2641 aaacatgctc aaagagtata tcaaatatgc tttttggtgt tttttgggat taaatgctct
     2701 atacttattt tgacaaatta tacagcaact ggtgattcta attctgtgac cagaagctgc
     2761 ttgattttat accaactttt tgagcttata ttgaatatca gctgtaataa tctgaccaat
     2821 aatatgactc gtgtttccta tggtttcaga actaatcacc aaaaaagata caaaaaaatt
     2881 acaaacatta tttaactttt tttataccta tttttcagta tttgcatttt ttttagctat
     2941 atttttaatg gcattatgac cagaagtagc tttagttttg tttgggaaaa aattcattct
     3001 atcatgaaaa atggtgatct tgtgagcact ttttacaatc gcaaatgtgt ttgtgggttt
     3061 caatttttct gttttagcat gattgtgaaa gatcaaagaa agagtagaaa tacttttcac
     3121 aacggtatta ataacggtcg tattatcggt tatatgaatg tatagtttat gagtttatgg
     3181 ggccgttctc acattatgaa taggagaaac gatactcttt attttaagtt ttaaattggt
     3241 ttataaagac ataaaatttg gagtaaactg gagatttata attaaaaata tatgtatagg
     3301 attattgttg tgaataattt tatggatgat aaagtcaaag atattcgtta atgatgattc
     3361 aatgagattg tttaatttat ggaggctgat tttaatagga ctaggatttt ttgttgtatt
     3421 tggattgttt aatttccaaa aagcacttgt attaaagtga gaaataaaaa agttgagaac
     3481 ttagcaaatg agttggaata caaaaacagt aaaattattt gctttttttt gaaatatgtt
     3541 taatatataa tcatatatga ttatattcta aacacaaagc atgaaaatca catcattatt
     3601 gaaaacaaaa aaaacagtat ttaaaattga tgacttttat caaattttat gattggaaaa
     3661 taaattttct ctaagaaatt taattactag acgaaataaa aaatgattac taaaaaatgt
     3721 tttttattga gtttggtgtt tgccaaacta taataagtac gaactagcta acatccttca
     3781 aaggccatct tatattagtt tggaaacagt tttgtaccag aaatgagtaa tttttcaaaa
     3841 ctattctagc acaatttcct ctgtatgatt gaaaactcgc aatataaata tatgaaatat
     3901 aacatattct tacaaaacaa taaaaaaaga aacattctac gaccaattgt gaattgttta
     3961 taaagataat tatgctattg ctacggtaga aagagcttta tgtgatatgt tatacttaaa
     4021 tccatcttac tattttgata atcttagagc tattaatcgg gagaaagttt ttgaaatagc
     4081 taaaatttat aataataaga gattaatttt agatttagaa aaaataaaaa atggcattga
     4141 atattaaaaa acacagaata ataatgatca atattttaaa agatattgtt ggttctgtct
     4201 actgaaaaca cttaggattt aaatgaggta cattattgta tcttttgtac aatttagata
     4261 gattttctac tgacttagat tttgacttat tgaacaaatg atcaataaat gaagaaaaaa
     4321 tgcttttaga tctagcaaaa atttgtagta aacatggtaa agtaaaagat atgtacaata
     4381 aagaaaaaac tttattttta ttagtagatt atgagcctta tgaaatgaaa ataaagattg
     4441 aaataaataa aaaaagatct attcatagca aatatgaata taaatctata ttgtgatatc
     4501 aggttttgtg tatggaggcg gaatctctat tcgcaaacaa gcttgtagca ttaactcaaa
     4561 gaaaaaatat agcaagtaga gatttgtttg atgtgcattt ttttctaaaa aaatgatttg
     4621 aaataaacga aaatataata aaagaaagaa ctggcaaatc tttaaagtct tatctaaaat
     4681 atgtaataga ttttataaca aaaaatttta cagaaaaaac acttttagct ggcttaggtg
     4741 aactagttga tcaaaaacaa aaatatttta tcaaaaattt attagtacaa gaaacttgtg
     4801 agtacttgaa attgattatt tcagactagt aaaaactaac aaaccaaatt tttgcttgta
     4861 cattaagaat gaaaaagtat tatgagataa atttagaaga aactgatctg gttacaaatt
     4921 gtagccagtt aaaattgatg gctcaagaca acagaaaatc taattgtgac gatttatcac
     4981 ggttctagag ataagtattt agatttatat tatattcaaa tataatataa atggtttata
     5041 tgctgtttga atataataat cacttattct atgatgcaat agcaaaatta gttgtctttt
     5101 tttcagccaa aatccttata ccagaaagta tgctagcttt ttcttgctct tgcagttgta
     5161 aatcataaaa attttgcata ttaagtcgag attgtgcaga tatacccatt gcagattcta
     5221 atcttaatgc ccaatctgcg ttcaagtttc tttttcaagt aatgataaaa ctcacctctt
     5281 gtgggctttt ttgtattaat tctgctagtt gtttttgagt ccatcctctc gcttctaatt
     5341 catcagctag aatttcacca ggatggataa gaatggctgt tttcatttga ctatcattaa
     5401 cgattaatat tgtagcaata tatatttgtt gataaattgc aatagaaaaa aattaactcc
     5461 ctctgaaaat ttgtagaaat atcttgcata agtttttcta aatagtatac ttcaagtaat
     5521 atttattttg ccataacatg ataagtaaag atgagcaatt tagcattatt tcaagaaaaa
     5581 gaaatccgtc gtcattggga cgcaaaacaa gaaaaacgat atttttcagt agtagatatt
     5641 gtaggagttt tatcagaaag taaagatcca agaaattatt ggaaagtatt gaaaaacagg
     5701 cttactaaag agtgaagtga gttggttaca aattgtaacc aactgaaatt agtagcttct
     5761 gatgggaaga aatatttgac cgatgtagca gatgtggaga ctattcttcg tcttgttcag
     5821 tctattccta gtcctaatgc agaacctttt aaagtctggc ttgcaaaggt cggttatgaa
     5881 agaatgcaag aaacagttga tccagcactt agtttggaaa gggcaagaga gaatcggaaa
     5941 aaacatggaa gaagtgaaaa gtggatccag caaagaatga tgggacaaga aacgaggaac
     6001 aaattgactg attatcgaca cgaacatgga attaaaaaat gagatgaata tgctattttg
     6061 acaaatatca tccatcaaga atggacttga atgacagttc aggagcataa aaaaatgaaa
     6121 aatctcaaaa cgcaaaattt gagagatcat atgtccgaag ctgaactgat ttttactgcg
     6181 ttggctgagc tttctacacg tcaaattgca gaaacgacac aagctacagg catgaaagaa
     6241 aatagagtag caagtaaaaa atgaggtact atcgccaaac aagcaagaaa aaaacttgaa
     6301 gaccaaacag ggaaatctgt gataagtgat cagaattttt taccacctaa aaagaagtaa
     6361 gtttttgtct cttaaataaa atcaatgaaa acagtcgtaa tcacttcttg atattataat
     6421 cctattcatc cctgacatat tgagtgcata gaactttgta aagaattggg agatgaactt
     6481 tgggtaatag taaataatga tgaacaagcc agggctaaaa cagggaaaca agaagttttt
     6541 caagatgaaa attttagaat gagagtcgtt tgagctatta aaggagtaga tcaagtcttc
//
LOCUS       DAOSID010000033         6441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_10886_length_6441_cov_15.180551, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000033 DAOSID010000000
VERSION     DAOSID010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10886_length_6441_cov_15.180551"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..2839)
                     /gene="mutS"
                     /locus_tag="PK674_02180"
     CDS             complement(197..2839)
                     /gene="mutS"
                     /locus_tag="PK674_02180"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOG15371.1"
                     /translation="MKYTPAIQQYIDIKKENPDCLLFFRLGDFYELFFDDAKLVSKTL
                     DLVLTSKNKNSKNPVPMAGIPYHSAEKYISKLISLGYKVAVAEQITEPKPGQIVEREV
                     VEIITPATYIKDSGSKNFSYILAITYKFFKNGENYHIAWGDFSLGEYNTKSFEKIEDA
                     KKFIVSINPVEIILDVDLPEKSALWDSLKKDLKSLISVYEIPFDLENYVLEQCKIQNL
                     GSYGQALESGRIDAFGLLLNYLKYTQKNNLNNIVRISFHSSKNHVLIDDITIKNLEIF
                     YNNYENLEKYSLVGVLDNTKTSAGARLLRKLFLNPINDLSKLKKRTENVQYYYDNLEK
                     TKKIHNIFGSVLDIQKIVSTILYKKLSPTIFTKLRYVLSIFFENNELMEELKRLGLSG
                     YHIESIENLYKILQKVLKNDEFIKNDMDFIADGYSAEIDRLRKIAYHSDEMLMDYQNE
                     LAKASKVSNVKLKYIINQGYFIEITNKDIQLFESNLASIVNTNNEKFNIVRRNTLKGA
                     QRYNSEFLEKIESKIIEAKNALIEQEFLILQELKDSIALIVNDLHDFSNYVSWLDLYT
                     AHAIFAKENNLIKADYLEKGSLEILEGKHLVVEKFLDKDQQFIPNNLLMSEGSFHNEL
                     GNKEDFGFVHIVTGPNMGGKSTYLRQNALILLMAHCGFFVPAKSAKIPLIDGIFARIG
                     SGDNLAKNQSTFMTEMIEVANILNNATKNSFIIFDELGRGTSTYDGLALTKAILEYVV
                     TEIKAKTLIATHYHELIALEEIYQGIKNYSVSVYETDKDVVFMKKIVKGGADKSYGLD
                     VAKLAGIPNVILQRAEENLSNLHSSTDKKNIKDFNQNLFDINRFAQVENPKYEKIKSI
                     LDSFDINNITPLQALQLLNKLKSL"
     gene            3152..4732
                     /locus_tag="PK674_02185"
     CDS             3152..4732
                     /locus_tag="PK674_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="GspE/PulE family protein"
                     /protein_id="HOG15372.1"
                     /translation="MKQITKSILDIPDILAIKPDFNSIKILKRDICEKTQTIVFATDG
                     NIFKVLSTNNFPEELNQIILKLEETGLSTEVFYTSTEGFQKAMKWYDDYEKMLQIQKE
                     EYDAQKQASGEGAIGMIKKIYKIRDSMEPGEFIMEVVRLAFQTGASDLHLQPQDDGVL
                     LRIRIDGVMQEILKFTHADFKKYLQKLKFIAGTKMNVDYVPQDGRFSFESVNRDGENK
                     KVDARVSFMPGMLSESTVIRFLDPTKGISTFEKIGFTGSTYEVLKRNLEKNTGIIIFT
                     GPTGSGKTTTLYTILNYLNDGKNKIITLEDPVEYELRGIQQSQINYNKGYTYAKGLKA
                     ILRHDPDIILVGETRDIETADIAINAALTGHKVFTTLHTNSAIESVPRLVNMGLQGYM
                     LAPSLNLIVAQRLVRKVCPHCSTKRDANFAEKSEIEDAIKKINDANPKINLKFNGKIP
                     QAIGCDKCNGSGYKGRLAVLETLEITEDIRNLIIKGKVGIDLYSKARESGYLTLREDG
                     IRKVLEGKTTLDEIRRVI"
     gene            5078..5800
                     /locus_tag="PK674_02190"
     CDS             5078..5800
                     /locus_tag="PK674_02190"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="ribonuclease HII"
                     /protein_id="HOG15373.1"
                     /translation="MEIYVDEAGRGPLAGPLHVGLILVLKQIHKKKFKDSKQLSSSQR
                     EKLFEKIKELEQKGSLLYSIGTVNNQEIDNLGLTKAISLAIKRGLFELLKKYYQNFLQ
                     YSLSKGLCSCDLINKFSIENLLHNKFSITNLKNLLQIISQTNPIFGMIIDGKQDFGVS
                     KDLEIKTKTVVKGDIKVHEISMASIVAKVSRDNRMIDCADKKYPQYNFKKHKGYGTKE
                     HISLIKKFGICKLHRKSFLKKI"
     gene            5876..6100
                     /locus_tag="PK674_02195"
     CDS             5876..6100
                     /locus_tag="PK674_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15374.1"
                     /translation="MNTFETEQFLEECGLDCSEQLGLDEESIKEFKSEALQTLYYDGN
                     SGTTCGKYFVGVKTFDALDELFRKPQNPEA"
     gene            complement(6179..>6441)
                     /locus_tag="PK674_02200"
     CDS             complement(6179..>6441)
                     /locus_tag="PK674_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15375.1"
                     /translation="IVWRDLCFVIDQEKDFGEVLNIAKNVEGVADLEIFDLYKGNNLP
                     EGKKSVAFKIKIKGENMQTENINEIMNKVIKQVESSGAKLRE"
BASE COUNT         2355 a          932 c          891 g         2263 t
ORIGIN      
        1 accctcctaa tcaaaccaaa atcagtaatt gcttttcact aaatctccat tttcatttta
       61 tagcttttct cttatcaatt catcgaataa caaaagtaat caaatttatc aaagccaaat
      121 atcatcaaac aacatagtac aacaccatat ttaaataata attaaacaat aaatctgaac
      181 tttataattt gtaatcttac aaacttttca acttatttag taactgtaat gcctgtagag
      241 gagttatatt atttatatca aaactatcta gaatactttt tattttttca tattttgggt
      301 tttctacttg ggcgaatcta ttaatatcaa acaaattttg attaaagtct tttatatttt
      361 ttttatcagt agaggagtgt aaattgctta aattctcctc tgccctttgt aaaattacat
      421 ttggtatccc agctaatttt gctacatcca aaccataact tttatcagct catcatttta
      481 caattttttt cataaacact acatctttat ctgtttcata aacacttaca gaatagtttt
      541 ttattcattg ataaatttct tccaaagcaa ttaattcatg ataatgagta gcaatcaagg
      601 tttttgcttt tatttctgtc accacatatt ctaaaatagc ctttgttaga gctaatcagt
      661 cataagtaga ggttcctctc cccaactcat caaaaattat gaaactattt ttagtcgcat
      721 tattcaaaat atttgctact tcaatcattt cagtcataaa agtagattga ttttttgcca
      781 aattatcacc actaccaatt ctagcaaata ttcaatcaat caacgggatt ttagcacttt
      841 ttgcaggcac aaaaaagcca caatgagcca tgagtaatat taaagcattt tggcgtaaat
      901 aagtagattt tcaccccata tttggtcctg taactatatg tacaaaacca aagtcttctt
      961 tatttccaag ctcattgtga aaacttcatt cgctcataag taaattgttt gggataaatt
     1021 gttgatcttt atccaaaaac ttttccacca ccaaatgttt tcattccaaa atttctaaac
     1081 ttcatttttc taaataatct gcttttatca aattgttttc ttttgcaaaa atagcatgag
     1141 cagtatacaa atccagccaa gacacatagt ttgaaaaatc gtgtaaatca tttacaatta
     1201 aagcaataga atctttcagt tcttgtaaaa ttaaaaattc ctgctcaatc aaagcatttt
     1261 ttgcctcaat tattttactt tcaatttttt ccaaaaattc tgaattgtac ctttgtgctc
     1321 attttaaagt atttcttctg acaatattaa atttttcatt gtttgtattg acgattgatg
     1381 ctaaatttga ctcaaatagt tgaatatctt tatttgttat ctctataaaa tatcattgat
     1441 ttattatata ttttaatttt acatttgata cttttgaagc ttttgctagt tcattttgat
     1501 aatccatcag catttcgtcg ctatggtagg ctatctttct caatctatca atttctgcac
     1561 tatatccatc agctataaaa tccatatcat ttttgataaa ttcatcattt ttcaatactt
     1621 tttgtaagat tttatataag ttttctatac tttcaatatg ataaccactc aatcataatc
     1681 tttttagttc ttccatcagc tcattatttt caaaaaaaat agacaataca tatctaagtt
     1741 ttgtaaaaat tgttggagat agttttttgt acaatatcgt gctaactatt ttttgaatat
     1801 ccagtacact tcaaaaaata ttgtgaattt tctttgtttt ttccaaattg tcgtaataat
     1861 actgcacatt ttccgttctt ttttttagtt ttgataaatc atttatcgga ttcaaaaaaa
     1921 gtttgcgtaa aagtctagca cctgcactag tttttgtatt atccaagact caaactaaac
     1981 tatatttttc aagattttca tagttgttat aaaatatttc taaatttttt atagtaatat
     2041 catctatcag tacatgattt ttggaactgt gaaaggaaat acgaacaatg ttgtttaaat
     2101 tatttttttg tgtatatttc aaataattta atagcagtca aaaagcatca atcctaccag
     2161 attccaatgc ttgcccataa ctgcccaagt tttggatttt acattgctcc aatacataat
     2221 tttctagatc aaaaggtatc tcgtaaactg aaatcaaaga ttttaaatct ttttttaaac
     2281 tatcccacaa agcagatttt tccggcaaat ctacatccaa tataatttca actggattta
     2341 ttgaaacaat gaactttttt gcgtcctcta ttttttcgaa actttttgta ttgtattctc
     2401 acaaactaaa atctcaccac gcaatatggt agttttctca atttttgaaa aatttgtaag
     2461 ttatagctag gatataagaa aaattttttg atccagaatc cttgatataa gtagcagggg
     2521 taataatttc tacaacttct ctttctacga tttgtccagg ttttggttct gtaatttgtt
     2581 cggcaacagc tactttgtat cataaagaaa taagtttgct tatatatttt tcagcactat
     2641 gatatggaat cccagccata gggacagggt ttttactatt tttatttttg gatgtcaaaa
     2701 ctaagtccaa agtcttgctg actaatttag catcatcaaa aaatagttca taaaaatctc
     2761 ataaacggaa aaacagcaaa caatcagggt tttctttttt aatatctatg tattgttgta
     2821 tagcaggagt gtatttcata aaagaactta taagctttaa gagacgaata tcaatgaata
     2881 aaataaaaaa caaaataaat agaaaattca gtacggtaaa tagctggatt tctaaaatat
     2941 tgttttattg atttgaatga tttttcaata tatttcaaga tatttttcag aatcaagagc
     3001 gacctttagc tacataatat atattatgtt ttcaaaacaa taaaagtcaa gttattttcg
     3061 tattagttta tcgatagttt tgcatctttt ttgcaatttt tttttttttg aatatactta
     3121 tcggtgattt atacattatt ttttacaaag catgaaacaa ataactaaat ctatattgga
     3181 tatcccagat atactagcta taaagccaga tttcaattca ataaaaatat taaaaagaga
     3241 tatttgcgaa aaaacacaaa ccatagtttt tgctacagat tgaaatatat ttaaagtact
     3301 ttcaactaat aattttccag aagaattaaa tcaaataata ttaaaattgg aagaaacatg
     3361 attgtctacg gaagtttttt atacttctac cgaatgattt caaaaagcga tgaagtggta
     3421 tgatgattat gaaaaaatgt tacaaataca aaaagaagaa tatgatgcac aaaaacaagc
     3481 ttcttgagaa ggagcgatct gaatgataaa aaaaatatac aaaataagag attctatgga
     3541 accatgagaa tttattatgg aagtggtaag attagctttt caaacatgag catcagattt
     3601 acacttacaa ccacaagatg attgagtttt gcttagaatt agaatagatg gagtaatgca
     3661 agaaatttta aaatttactc atgccgattt caaaaaatat ctacagaaat taaaatttat
     3721 agctgggaca aaaatgaatg ttgattatgt acctcaagac ggaagatttt cttttgaatc
     3781 ggtaaataga gattgagaaa ataaaaaagt agatgcgaga gttagtttta tgcctggtat
     3841 gctctcggaa agtacagtaa ttagattttt agatcctact aaatgaatat ccacattcga
     3901 aaaaatctga tttactggca gtacttatga agtgttaaag cgtaatttag aaaaaaatac
     3961 aggaattatt atcttcacat gaccgacttg atccggtaaa acaacaacat tatatacaat
     4021 tttgaattat ttaaatgatt gaaaaaataa aataataact ttggaagacc ccgtggagta
     4081 cgaacttcgt ggaatccaac aatcacagat aaattataat aaatgatata cttatgcaaa
     4141 atgattaaaa gcaatactta ggcatgatcc agacataata ttagtatgag aaacgagaga
     4201 tattgaaact gcggatattg ctatcaatgc tgctttgaca ggacataaag tttttacaac
     4261 tttgcataca aattctgcta tagaaagtgt tcctagatta gtaaatatgt gattgcagtg
     4321 atatatgctt gctccttcat taaatctaat tgtggctcaa agacttgtca gaaaagtttg
     4381 tcctcattgt agtacaaaaa gagatgccaa ttttgcggaa aagagtgaaa ttgaggatgc
     4441 tatcaaaaaa attaatgatg caaatccaaa aataaattta aaatttaatt gaaaaattcc
     4501 tcaagcaatt tgatgtgata aatgtaatgg tagcggttat aaatgaaggc tagctgtttt
     4561 ggaaactctt gaaattacgg aggatatcag aaatttaatt attaaatgaa aagtaggaat
     4621 agatctgtat tccaaagcta gagaaagtgg ttatttgact ctaagagaag atggaattag
     4681 aaaagtgttg gaatgaaaga ctactttgga tgaaattagg agagtgattt aagggcggga
     4741 tgaacttcgt tcgggataaa gtgataaaaa gttcgcatcg cttaagcggt gtgaactttg
     4801 ctcacgataa aatgataaag tgatgaaacc ccgcttagcg gggtgaatga taaaatgatg
     4861 aaatgtttaa atgataaaat aaatattctt gatttctggc aggcgcgaat tcgaattcgc
     4921 gcctacattt tttttattat tggctagcac aacgaacagc acccgggtgg taggataata
     4981 ttaaaatgaa acaaatgtaa attgaaacaa attgccattg aattatagaa tctaaatcat
     5041 agagtgtaat gcatgaaatt tacataatac attaactatg gaaatatatg ttgatgaagc
     5101 gggtagggga cctttagctt gacctttgca tgtttgatta atcttggtat taaaacaaat
     5161 tcataaaaaa aaatttaaag atagtaaaca gttatcctca tcgcaaagag aaaaactttt
     5221 tgagaaaatc aaagaattgg aacaaaagtg atctttactt tacagtatat gaacagtaaa
     5281 taatcaggaa attgataatt tatgactaac aaaagctatt tctttggcca taaaaagatg
     5341 attgtttgaa ctattaaaaa aatattatca aaactttcta caatactcat tgtcaaaatg
     5401 actttgtagc tgtgatttaa taaacaaatt ttctattgaa aatcttttac acaacaaatt
     5461 ttctatcaca aacttaaaaa atttgctaca aataatatcc caaacaaatc caatatttgg
     5521 gatgataatc gactgaaaac aagatttttg agtatcaaaa gatttagaaa taaaaacaaa
     5581 aactgttgta aaaggggaca ttaaagtcca tgaaatatct atggcttcca tagtagcaaa
     5641 agtttctaga gataatcgaa tgatagattg tgcagataaa aaatatccac aatacaattt
     5701 caaaaaacat aaatgatatg ggacaaagga acacatcagt ttaatcaaaa aattttgaat
     5761 ttgtaagtta catagaaagt cctttttaaa gaaaatataa aaaacagttg acatctgttt
     5821 gcaatttcat ataatataga tggtgaaaat atttttatat attaatacta catagatgaa
     5881 tacatttgaa acagagcagt ttttggaaga atgctgatta gattgttcgg aacaattagg
     5941 tctagatgag gagtctatca aggaatttaa aagcgaagca cttcaaacat tgtattatga
     6001 tggtaatagt ggtactactt gttgaaaata ttttgtttga gtaaagacct ttgatgcatt
     6061 agatgaacta tttagaaaac ctcaaaatcc agaagcatag gtataaatga taacctacca
     6121 cccgggtgcg attggttagc actgcgaaca gcaacccggg cggtaggaaa caaacttcct
     6181 actccctcaa ttttgctcaa ctactttcta cttgttttat gactttattc ataatttcat
     6241 ttatattttc agtttgcata ttctctcact taatttttat cttgaatgcg actgatttct
     6301 ttcattctgg taaattattt cacttataca aatcaaaaat ctctaaatca gctactcatt
     6361 ctacattttt tgctatattc aagacctctc aaaaatcctt ttcttgatct ataacaaaac
     6421 aaagatctct ccaaacaatt t
//
LOCUS       DAOSID010000034         6129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_11599_length_6129_cov_10.921962, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000034 DAOSID010000000
VERSION     DAOSID010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11599_length_6129_cov_10.921962"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..862
                     /locus_tag="PK674_02205"
     CDS             218..862
                     /locus_tag="PK674_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15376.1"
                     /translation="MIETKVLGSDNLSDTKELLDKNIDKNNFYKKDILNLENNIVNQE
                     KAEKLLSSLKGNESFKKFSNIWDSFSDKEKLDVYNDGAYMFKITGGLRRNVNFINPIK
                     SPFRSPLIRAFQKGSIKEQMTKDSFYVMSAATRSFVHFGLLSKPNDLTQEDLIKNIKK
                     DGKNVNMTMNVLEKTAMVVPQLRPVLPVIKTIKPIVKSSSNMTTDLMLKANQTA"
     gene            1075..2940
                     /locus_tag="PK674_02210"
     CDS             1075..2940
                     /locus_tag="PK674_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15377.1"
                     /translation="MVDGQTNILDSTADLQNKQPELEGLQDTISDANNDLAELKNSLG
                     TLDDSAKLVKGLELKSTIENIRQELDTLKKDSSNNADKIKQLESQYKKLNAEFDRTIG
                     NQLSSLEASIKKSNVSPDSKDVDDKEKAEKRKKRRWIFGSIGALSIGGILLATRKRRK
                     ERREARRAERAKNKATKETKDRKPFWDRWYGKVLKGVGIGTGIYYLAHGIYTGNWHLR
                     DIFDRERGKKISFEDALAVAEGNIANQTDHESMSYDIDLKYLEDTSEIQAYGEKIKID
                     KKRRKIDGLNIKFKKYEHLIATAIVVAFLKQNYSGMCARPKPFNLGSSWRGNINVNTG
                     DGSETAVDGTGISGKITGIGLAGILAIVSGIYGGLKLGMTVGIVGGIVSVVGGHLYDS
                     DNILNKYMPELDNEYGKKALSAYLNRMDCWQQKNQTKDDITESPIKEEVIECMNEIQK
                     TAEDKEYSKGERRKLDAIPDPNDKTKYTIKAYNRDFKAEVLGTEGNRTIKILGISGGY
                     PSINVDPSIAGFSDMQLPLKEGIHMAIFIGSMLDQYPHKGTKSPRFKYRTPVGVLNYG
                     VFFNDGTNGGYGTRVLKKDKFKQRMPTLFEQKDSLIRFLNEGVTCENNISFRKKT"
     gene            3070..3939
                     /locus_tag="PK674_02215"
     CDS             3070..3939
                     /locus_tag="PK674_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15378.1"
                     /translation="MVKNIHIKVEKYIEDREKLSSLSGIKESLKNLGLDLKIGEWLKK
                     DKDMLEYAKLFFKEETENTKRTTKEELKFLKLEIKLRFKCSYFADFKEFLKNLKESEN
                     STEKSAEGNKHTEQLEQEEIANKTFLTTPISQIKSHPYYKSSKSGVTRCSATAQFNGD
                     SFGLELPKGDAYKAGKNPGAGCLATIPSNKKTETPKNSWKNLSLSDFTGQTNENINFA
                     DIYTNSKSGYGHRVVGFKDSDGQWYVLDPYTNVNGLDDKPKKLEDYMGKRKIKKIHFY
                     QSDGYKFENAQYV"
     gene            complement(4308..4985)
                     /gene="aqpZ"
                     /locus_tag="PK674_02220"
     CDS             complement(4308..4985)
                     /gene="aqpZ"
                     /locus_tag="PK674_02220"
                     /inference="COORDINATES: protein motif:HMM:NF003838.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="aquaporin Z"
                     /protein_id="HOG15379.1"
                     /translation="MKKNLAEFLGTFWLVLGGCGSAVLAASFPDLGIGLLGVSLAFGL
                     TVLTIAYSMGHISGAHLNPAVTIGLWMGGRIKAKEILPYVIAQILGAIVAAGVLYLIV
                     TGNGSEIGSFAANGYGIHSPGGYGVVAAIITELVMTFMFLLVILGATDEKAPKEFAGL
                     AIGLALTLIHLISIPVTNTSVNPARSISQALFVGDWALSQLWLFILIPIVGAILAGLV
                     YKFLKSK"
     gene            complement(5152..>6129)
                     /gene="miaA"
                     /locus_tag="PK674_02225"
     CDS             complement(5152..>6129)
                     /gene="miaA"
                     /locus_tag="PK674_02225"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOG15380.1"
                     /translation="LYKLSNSMNFQDIVLGSKTEIEKFLEKNPKGIVVIRGATATGKS
                     KLSIELSKFFDIEIISSDSRQIFRKMDIGTDKVSDEILNQIPHHQVNIIDPDQVYTSG
                     EWQKDVKKQIKEIQSRGKIPFIVGGTGLYIDTIYKNFDMPEIGPDLDFRKEMFDLENK
                     EAGILHKRLSEIDPEEAKKHHPNSTRYIVRALEIFHKSGKTKTDTFKEQNVEQPILMI
                     GIRRDKESTNELIAKRIKEMINGGLIEEVKGLLEEGYSPTLQSMQGIGYKEVVGYLEG
                     EYDIEEMENLLNKNTQYLAKKQRTWFRRYIEDGEKHPKKDVSYKVWYLD"
BASE COUNT         2365 a          861 c          923 g         1980 t
ORIGIN      
        1 atagacactc gttgaagtag gaagctcaac catctttagt ggttgagtag ttcacataaa
       61 aaaacagtaa ataaagtagt tagctttcac tattccctat cattttagcc cccgtttttt
      121 ttaattatgc tgaatgaagt aaaaagtagt caaaagtttg acaaatcaaa cacttatatt
      181 tataatacaa tagtttttta tttattaaaa ataaataatg attgaaacta aagttttatg
      241 atcagataat ttatctgaca caaaggagtt gttggataaa aatattgata aaaataactt
      301 ttacaagaaa gatatattga atcttgagaa taatatagta aatcaagaaa aggctgaaaa
      361 attactatcc tcacttaaat gaaatgagag ttttaaaaag ttttctaata tctgggattc
      421 tttttcggat aaagagaaac tcgatgttta caatgattga gcttatatgt ttaaaataac
      481 atgaggtttg agaagaaatg taaactttat aaatccgatt aaatctccat ttagaagccc
      541 tttaattaga gcttttcaaa aaggttctat aaaagaacag atgacaaaag attcttttta
      601 tgttatgtca gctgcaacaa gaagtttcgt ccatttttga cttttaagta aacccaatga
      661 tttaacacaa gaagatttaa taaaaaacat taaaaaggat tgaaaaaatg taaatatgac
      721 aatgaatgtt ctagaaaaaa cagccatggt tgtcccacag ttgagaccag tacttccagt
      781 aataaaaaca ataaaaccta ttgtgaaatc ttcatcaaat atgactacag atttaatgtt
      841 aaaagctaat caaactgcat aaattttctt taatttttca aaaatttgta aagttattat
      901 acatctggga tatctatctg agagaatatt actaagatat catagataat atacaagcta
      961 tacattatac aacaaaaatg tcaattctta atgtgcaaaa tatcggttat ttttgtttaa
     1021 tatttttttt tatttataat aaattgattt atatttatta cataaacaac aaaaatggta
     1081 gattgacaga caaacatcct tgatagtaca gcagatttgc aaaataagca accagaatta
     1141 gagtgattgc aagatactat tagcgatgct aataatgatt tagcagaatt aaaaaattca
     1201 cttggtactt tagatgatag cgcgaaatta gtaaaatgat tagaattaaa atcaactata
     1261 gaaaatataa ggcaagaact agatacatta aaaaaagatt cctctaataa tgctgataaa
     1321 attaaacaac tagaatctca atataaaaaa ttaaatgcag aatttgatcg tactatttga
     1381 aatcaacttt catctttaga agcttctatc aagaaatcaa atgtttcacc agattcaaag
     1441 gatgtagatg ataaagaaaa agcagaaaag agaaaaaaaa gacgatggat attcggttca
     1501 attggagcat tatctatttg atgaatatta cttgccacca gaaaaagaag aaaggaaaga
     1561 agagaagcta gaagagcaga aagagcaaaa aataaagcaa caaaagaaac aaaagataga
     1621 aaaccatttt gggatagatg gtatgggaaa gttttgaaat gagttggaat tggaacagga
     1681 atttattatt tagcacatgg tatatatacg gggaattggc atctaagaga tatcttcgat
     1741 cgagaaagag gaaagaaaat aagttttgag gatgccttag ctgtagcaga gtgaaatata
     1801 gcaaatcaga cagatcatga aagtatgtct tacgatattg atttaaagta tttggaggac
     1861 acatcagaaa tccaagcata ttgagaaaag attaagattg ataaaaaacg taggaagata
     1921 gactgattga atataaagtt taaaaaatat gaacatttaa tagctacagc gatagtagta
     1981 gcatttctga aacaaaatta ttctggaatg tgtgcgagac ctaaaccgtt taatcttggt
     2041 agtagttggc gtggaaatat aaatgttaat acatgagatg ggagtgagac agcagtagat
     2101 tgaactggta tttcatgaaa aataactgga ataggattag catgaatatt agctatagtt
     2161 tcatgaatat attgatgact aaaactttga atgactgtat gaattgttgg tggaatagta
     2221 tcagtagtgg gtggtcatct atatgattct gacaatattt taaataagta tatgccggag
     2281 ctagacaatg aatattgaaa aaaagctctt tctgcctatt taaataggat ggactgttgg
     2341 caacaaaaga atcaaactaa agacgatatt acagagagtc cgataaaaga agaagtgatt
     2401 gaatgcatga atgaaattca aaagacagca gaagataagg aatatagtaa aggagagcgc
     2461 agaaagctag atgcgatacc agatccaaat gataaaacaa aatataccat aaaagcatat
     2521 aatagagatt ttaaagcaga ggtgttgtga acagagtgaa ataggacaat caaaatacta
     2581 tgaatctcat gatgataccc ctcaataaat gtagatccgt caattgctgg attttctgat
     2641 atgcaactcc cattaaaaga gtgaatacac atggcgatat ttatttgatc tatgctagat
     2701 caatatcctc acaagtgaac aaagtctcct cgatttaagt atagaacacc agtatgagtt
     2761 cttaattatg gggtgttctt taatgatgga actaattgat gatattgaac aagagtttta
     2821 aaaaaagata aatttaaaca aaggatgccc actttatttg aacaaaaaga ctcattgatc
     2881 aggtttttaa atgaaggggt aacttgtgaa aacaatataa gttttcgaaa aaaaacataa
     2941 aaattagctg ccaaatgaga gataggtaaa aaatatttta aataaagagt tactcaatcg
     3001 ttatgtgaaa ataattggtg aagaattaaa tagaaaataa aaatttatct tacttaaatt
     3061 cattataaaa tggttaaaaa tattcatatt aaagtagaga aatatattga agacagagaa
     3121 aaactatcgt ctttatcagg aataaaagag agtttaaaaa atctttgatt ggatctaaag
     3181 atttgagagt ggctaaaaaa ggataaagat atgttggaat atgcaaaact tttttttaaa
     3241 gaagaaacag aaaatacaaa aagaacaacc aaagaggaac taaaattttt aaaattagag
     3301 attaaactga gattcaagtg ttcttatttt gctgatttta aagaattctt aaaaaactta
     3361 aaggagtcag aaaactctac cgaaaaatca gcagagggaa ataagcatac agaacagtta
     3421 gaacaagaag aaatagcaaa taagacattt ctcactacac ctatatctca aataaaatct
     3481 catccatatt acaaaagttc aaaatcaggt gttacacgat gttcagcaac agcacaattt
     3541 aactgagata gttttggatt agaactccct aaatgagatg cttataaagc tggtaaaaat
     3601 ccatgagcag gctgtcttgc aacaatacca agcaataaaa aaactgaaac acccaaaaat
     3661 agttggaaaa acttatcttt gtcagatttt acaggtcaga caaatgaaaa tataaatttt
     3721 gcagatattt atactaactc aaaatcttga tactgacata gagtcgtgtg attcaaagat
     3781 agtgatggac aatggtatgt attagatccc tatacaaatg taaactgatt agatgataaa
     3841 cctaaaaaat tagaggacta tatgtgaaaa agaaaaatca aaaaaattca cttttatcaa
     3901 tctgattgat acaaatttga aaatgcacag tatgtttaag gtttatgtat taaacattag
     3961 tcatactaga tacaaaacaa atgaatataa aaaaaacaaa agaatcagaa tctaattttt
     4021 tattttcttt gaatttactc tttcccgaac aaggaggatc tattaatttt acttgaagtt
     4081 cctttaatta ttcacatccc cttttttgct aaaaaatctt ttttaaattg ccacaaattc
     4141 atagtttatt ttttataatt cccctccaat aattgagaga gataacatat cggaacaaac
     4201 attatttttg ataacatgtt ttataaatct tatttttacc aaaaacaaag caaaattaag
     4261 tttataatag ttattgttca cggtgagcat aaaaaaatag tactacttta ttttgatttt
     4321 aggaacttat atacaagacc agctaaaatt gcacctacga ttgggattag aataaatagc
     4381 cataattgag aaagagccca atcaccaaca aacaaagcct gacttatact tcttgctgga
     4441 tttactgatg tgtttgttac tggtatgcta atcaagtgaa taagagttaa tgcaagtcca
     4501 attgctaaac cagcaaattc ttttggtgct ttttcatctg ttgctcccaa aataaccaat
     4561 aagaacataa atgtcataac taattctgtt ataatagcag caactacacc atatcctcct
     4621 ggagaatgta ttccatatcc atttgcggca aaagaaccaa tttcacttcc attccctgta
     4681 acaataagat aaagaactcc tgcggctaca attgctccta aaatctgagc tattacataa
     4741 ggcaaaatct ctttagcctt aattcttcca cccatccata aaccaattgt tacggctgga
     4801 tttaagtgtg cgcctgaaat gtgacccatt gaatatgcaa tagttaggac agtaagccca
     4861 aatgctaatg acactcctaa aagtccaatt cccaaatctg ggaaacttgc agcaagtact
     4921 gcacttccac aaccacctaa tactaaccaa aaggttccta aaaattctgc taggttcttt
     4981 ttcatttttt aataaattaa aaaagtaaat ttttaccttt tatgcctttt tttactagtt
     5041 ttgcaaggaa aaatgtatag gtacggaacc ttcttagtcg aattaaaaga atccattaaa
     5101 agttctgagg tcccattatt ttgaaattat agttgtaagt agttaaaaca cctaatccaa
     5161 gtaccaaacc ttatatgaaa catccttttt aggatgtttt tctcaatctt caatatatct
     5221 tctaaaccaa gttctttgtt ttttagctaa atattgtgta tttttgttta atagattttc
     5281 catttcttct atatcatatt ctcattccaa atatcccaca acctctttgt atcatattca
     5341 ttgcatagat tgtaaggtag gggagtatca ctcttctaaa agtcacttaa cttcttctat
     5401 caatcatcaa tttatcattt cttttatcct ttttgcaata agttcgttag tactttcttt
     5461 atctcttcgt ataccaatca ttaaaattgg ctgttctaca ttttgttcct taaaggtatc
     5521 tgtttttgtt tttccagact tatgaaaaat ttctaaagct ctaactatat atcttgtact
     5581 gtttggatga tgcttttttg cttcttctgg atctatttca gataatcttt tatgtaaaat
     5641 tcatgcctct ttattttcca aatcaaacat ttctttccta aaatcaagat ctggtcatat
     5701 ttctggcata tcaaaatttt tgtaaattgt atcaatatat aatccagttc ctcaaactat
     5761 aaacggtatt tttcaccttg attgaatttc ttttatttgt ttttttacat ccttttgcca
     5821 ttctcctgaa gtatacactt gatctggatc tattatattt acttggtgat gaggtatttg
     5881 attcaatatc tcatcagata ccttgtctgt acctatatcc atttttctaa aaatctgtct
     5941 gctgtcacta ctaattatct caatatcaaa aaattttgat aactcaattg atagcttact
     6001 tttaccagta gctgtagctc atcgaattac cacaattcat ttaggatttt tttctaaaaa
     6061 tttttctatt tcagttttac ttcatagtac tatatcctga aagttcatgg agtttgaaag
     6121 tttataaag
//
LOCUS       DAOSID010000035         6094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_11684_length_6094_cov_10.869515, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000035 DAOSID010000000
VERSION     DAOSID010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11684_length_6094_cov_10.869515"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PK674_02230"
     CDS             <1..413
                     /locus_tag="PK674_02230"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=25
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG15381.1"
                     /translation="DVFGFNTAISQLMIFVNELNTKEYISKDTFEKLTILLAPFAPHL
                     AEELWSELGNQSSIFHQGVWPKCDEKYLVDDVLTIAVQVNGKVRGTIEISPDSTQEQV
                     FALVKENEKISKYLDGEILKIIYIPGKICNIVVS"
     gene            694..1752
                     /locus_tag="PK674_02235"
     CDS             694..1752
                     /locus_tag="PK674_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15382.1"
                     /translation="MTILLLFTFFYIVFSDAVNTRYTEFHFLIGDKFYDHITVNLTGA
                     ETGNALIYLENKSETQLDGVFSFVDGDIIEYGSTGVRVCKSDNEVDVFGQYVTMNSNT
                     FSIPPYEAITGFLDLCFPAGYSGTYYGCIVYHPNISESDGNSLNTVPRKSIFLDVNVG
                     LVDSLFRFKIYPSDRSNPKKKGLSGTILFYEKSNTEQAVLSRQIQTNSNGTGEFFASL
                     PNGEYFIVFKGLAHLASYLSGFAIVGNAGIELDFTTGDNLFYTKPYGDDGYRYQIAGD
                     LRNINGLYDGQINTNDIATLFSTGLCLFNTQIPVDQYHPCNLNGDSLVLMSDLGVIVA
                     NIMENGPFFQQAPLFGGF"
     gene            1777..3486
                     /locus_tag="PK674_02240"
     CDS             1777..3486
                     /locus_tag="PK674_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15383.1"
                     /translation="MNVKLNLKLKVFVYISLLFSVFFAYSFSNEFHLIPISGTTIRHY
                     CNYPIQIKIKKGDYSFTTFSSIIKFSTGEIIINPATIDINPFFGLGSKWSITGNLFSA
                     EGGSLNPFNDSMEDVVNFDIKTKEGFTGDLTSLRFVNFEGNDPEFSPENTDDGITITS
                     NDIVGDTLSGVTNATYNIIARPCKEDINPPIVFSGSWSPNISGGKLEGIHTIRLLVLD
                     WIGKHGHYWYQGSGANNMNNYVLAPASVDNQNGVNNLTIRVRINNGGNIEYPEFDMDF
                     YTGSNPSEWTWNSKRRGYWVEFQNTIPFEIEKQVTITITGYDNYNVSGNRYLMNQSFV
                     FNSSDNPTINIITPPHASSGQNYILSSIDFVVSDSWAGVDTDKVFITIPSIYSGTILL
                     LTGYTYSGSDLQFTLITGSSGFGEAGSYDVKLVPKGKFPSNTRINITGMVVDLAGNTG
                     FGNWHFNTKPSCEDLGCFSILNINFLSGPSFGYNPRLFTGQYLIVTGLSYNTAYPYFT
                     GTNGDTLVCGFDYEGTVLTGNVDIYSNTGIIINGHFYTGMNLYVTGLDFVYNNGVITV
                     LDD"
     gene            3515..4234
                     /locus_tag="PK674_02245"
     CDS             3515..4234
                     /locus_tag="PK674_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15384.1"
                     /translation="MKKLITFVLFTLFFGASSLGASISISPESGHFGKNCVMEFDIIV
                     ESLSDNILTVDVYLESSMEYVDFVPSNIFKYYLPPIIEGNKIYLGAFSESGVKFVGKE
                     KFGTLYLKSDDVSLDGYVNFDFRGQGNTTDTNLNLIGGIDVLDEVKNGTYYFDLDPCE
                     QNIVDDVVSDIEGGFSSIDEEQYVQDMISEFNKRGVAQKVSLFFSKYWRSFALFVLVL
                     ALIYVFIKNKKSLSKGKKLKK"
     gene            4213..>6094
                     /locus_tag="PK674_02250"
     CDS             4213..>6094
                     /locus_tag="PK674_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15385.1"
                     /translation="MKEIKKIIYAVFLLSVLWVYVDSQYYYFSGPINSDGYQQGCSKE
                     LEIRFDTKTGKASAGIFWLKLDDTKINYVTGGSLDSDLFVGNPLGFSDPVYMGRHHDS
                     GYDPYTVLNIQRRNDFGVESTGDRPFGVVKFVPKYHVTGYTVDFGMVYISGSNWTTET
                     TLSFEGADIINPSQQALRLTGTIEVEQKPCTLDTVAPIVSDKNYVNGSTRNINSGLNV
                     ELTDSNGTEVPYVWRNGAWTGNIWGINNQYGVDTSTINVNVSGNGYSVTLYSNNFHIN
                     PLGTGKTWQDLDRDYKITLSSGELFDFGIEKLITIKYIVEDRAGNKANAIHLFNQPKN
                     PWVSEVSPSNNQSDVASNSVIRFNVHDDRAGVDSGTINLVLSGRGNTFYANYSYLDFE
                     LTPIGGSANFDDYNVVLQSHAPFPSSGIIAMSVSGYDLAGNGGLLYSGQFFTKASCED
                     FGCSSTNMVKISTGNLLNFINYYHNVLYISGGVNPSLIRDGGNTGYINCNLQNNFLSI
                     YGGGVGGTGWEFRGKSAFDELQIIGDDVIATLSGDVLTLERINVYTPPPITTDNIDGE
                     RIKTDFLVELIAEATSGQGVANTYYCTGIDTSCTPYIIGTPPVTTFTVTCPNNTTCPS
                     QYV"
BASE COUNT         1986 a          728 c         1090 g         2290 t
ORIGIN      
        1 tagatgtttt tggttttaat acagcaatat ctcagttgat gatttttgta aatgaattaa
       61 atacaaaaga atatatttct aaagatactt ttgaaaagct gactatactt ttggcacctt
      121 ttgcacctca cttagcagag gaattatggt cagaactatg aaatcaatct agcatttttc
      181 atcagggagt ttggcctaag tgcgatgaaa agtacttggt agatgatgtt cttactatag
      241 ctgtgcaagt aaactgaaag gtaagatgaa ccattgaaat tagtccagac tctactcaag
      301 agcaggtgtt tgcactggtt aaagaaaatg aaaaaataag taaatactta gactgagaaa
      361 tattgaaaat aatttatatt ccttgaaaaa tttgtaatat tgttgtaagc taatttgttt
      421 gttttaacta tgatcttgta acaaaaacat gttttgtaaa gtagattttt tgtttttgac
      481 aaattagaca ttagttattt tgcttgtttt tttaaccgtt tgggaactaa attattactt
      541 gaataaattt gtctacttta atcggttgtc atttgctgtc tatatctaaa aggcttgaaa
      601 tatttctgtg atatgttaaa tatgtttttg taaaatttac aattttttat tttactatag
      661 tattgtatga gtattttcaa aaaggtattt ttgatgacaa ttctgttatt atttacattt
      721 ttttatatag ttttttctga tgcagtaaat actaggtata cagaatttca ttttttgatt
      781 ggagataagt tttatgacca cataacagta aacttgactt gagcagaaac aggtaatgct
      841 ttgatttatt tggagaataa gtctgagaca caacttgatt gagtgttttc ttttgtagat
      901 ggcgatataa ttgaatattg atctacttga gtaagagttt gtaaaagtga caatgaagta
      961 gatgtttttt gacagtatgt aacaatgaat tctaacactt tttcgatacc accatatgaa
     1021 gctattacag ggtttttgga cttgtgtttt cctgcctgat atagtggaac ttattattga
     1081 tgtatagttt atcatcctaa tattagtgaa agtgatggta acagcttaaa tactgttcct
     1141 cgtaaatcta tatttttgga tgtaaatgta ggattggtag atagtttgtt tagatttaag
     1201 atttatccgt cggatcgttc aaatcccaag aaaaagtgac ttagttgaac tatacttttt
     1261 tacgaaaaaa gcaatactga acaggctgta cttagtaggc aaattcagac aaattccaat
     1321 tgaacttgag aattttttgc tagtctacca aattgagaat attttattgt attcaaagga
     1381 ttggctcatt tagcatcgta ccttagttga tttgctattg tttgaaatgc tggtatagag
     1441 cttgatttta ctacatgaga taatttattt tatactaaac catatggaga tgattgatat
     1501 cgttatcaaa tagctggaga tttgagaaat attaattgac tctatgattg acagataaat
     1561 acaaatgata tagcaactct ttttagtact ggcctttgtt tatttaatac tcaaattcca
     1621 gttgatcaat atcatccttg taatttaaat ggtgattctt tagtgcttat gtcagatttg
     1681 tgagttattg ttgctaatat aatggaaaat tgaccattct ttcaacaagc tccgttgttt
     1741 tgatgatttt agattttata ttttgttagt attcttatga atgttaaatt gaatcttaaa
     1801 ttaaaagttt ttgtttatat ttctttattg tttagtgttt tttttgctta ttctttttct
     1861 aatgaatttc accttattcc aatttcatga actaccatta gacattattg taattatcca
     1921 atacagataa aaataaaaaa atgagattat tcatttacta ctttttcttc aataataaaa
     1981 tttagcactt gagaaattat tataaatcct gctacaattg acattaatcc atttttttga
     2041 ctgggttcaa aatggagtat tactggtaat ttgtttagtg cagaatgagg aagtttgaat
     2101 ccgttcaacg attcaatgga agatgtggtt aattttgata taaagactaa agagtgattt
     2161 actggagatc ttacatcgtt gagatttgtt aactttgaat gaaatgaccc tgaattttca
     2221 ccagaaaaca cagatgatgg aattactata acttctaatg atattgtttg agatactcta
     2281 agttgagtta caaatgcgac atataatatt attgcgcgtc cttgtaagga ggatataaat
     2341 cctcctattg tatttagtgg ctcttggtca ccaaatataa gttgatgaaa actggaatga
     2401 atacatacta ttagactttt ggttcttgat tggatatgaa aacatggaca ttattggtat
     2461 caaggatcat gagcaaataa tatgaataat tatgtgttag ccccggctag tgtagataat
     2521 caaaatggag taaataattt aactataagg gtacgcataa ataattgatg aaatattgaa
     2581 tatccggaat ttgacatgga tttttatact ggttcaaatc caagtgaatg gacttggaat
     2641 agtaaaagga ggggatattg ggttgaattc caaaatacaa taccatttga aattgaaaaa
     2701 caggttacaa ttacaattac atgatacgat aattataatg tttctggtaa tcgttattta
     2761 atgaaccagt ctttcgtttt taactcttct gacaatccga ccatcaatat tataacacca
     2821 cctcatgctt ctagtggtca aaattatatt ttgtctagta ttgattttgt tgttagtgat
     2881 tcttgggcag gagtagatac ggataaggtg tttattacta taccaagtat atattcgggc
     2941 acaatactgt tgttaactgg atatacctat tcatgatccg acttgcaatt tacattgatt
     3001 acttgaagtt cagggtttgg tgaagctggc tcttatgatg taaaattggt ccctaaatga
     3061 aaattcccat ctaatacaag aataaacata acaggaatgg ttgtagatct agcttgaaac
     3121 acagggtttg gtaattggca ttttaatacc aagcctagtt gtgaagattt gtgatgtttt
     3181 agtatattaa atataaattt tttgtctgga ccgtcctttt gatataatcc acgattgttt
     3241 acctgacagt acttaatagt tacctgatta agttataaca ctgcttatcc atattttaca
     3301 tgaactaatt gagacacttt ggtttgtgga tttgattatg aggggactgt tttgacatga
     3361 aatgttgata tttattcaaa tacttgaatt attataaatg gtcattttta tacttgaatg
     3421 aatctgtatg ttacttgatt agattttgta tacaataatt gagtgattac ggtacttgat
     3481 gactagtttt tttacttctt gttaaaaaat ttatatgaaa aaacttataa cctttgtatt
     3541 gtttacatta ttttttggag ctagttcttt gggagctagt atttctattt ctcctgaaag
     3601 tggtcatttt ggtaaaaatt gtgttatgga atttgatatt attgtggaat ctttatctga
     3661 caatattctt acagtggatg tttatttgga aagttctatg gaatatgtag attttgttcc
     3721 ttcaaatata tttaagtatt atttaccgcc aattattgaa ggtaacaaaa tttatttatg
     3781 agctttttca gagtcatgag tgaagtttgt ttgaaaggaa aagtttggta cattgtattt
     3841 aaaaagtgat gatgtgagtt tggattgata tgttaatttt gattttagat gacagggaaa
     3901 tacaactgat acaaatttaa atttgatttg atgaatagat gtgttggatg aagttaaaaa
     3961 ttgaacttat tactttgact tggatccttg tgaacagaac attgtagatg atgttgtaag
     4021 tgatatagaa tgatgatttt cttctattga tgaagaacaa tatgtacaag atatgatatc
     4081 tgagtttaat aaaagatgag ttgctcaaaa agtttcatta tttttttcta aatattggcg
     4141 aagttttgca ttgtttgtgt tagttttagc gcttatatat gtttttatta aaaataaaaa
     4201 atctttaagt aaatgaaaga aattaaaaaa ataatatatg ctgtattttt gttgtctgtt
     4261 ttgtgggtat atgttgactc acaatattat tatttttctg gaccaataaa ttctgattga
     4321 tatcaacagg gatgttcaaa agaattagaa attaggtttg atacaaaaac gggaaaagca
     4381 agtgcaggaa tattttggtt gaaattggat gatacaaaaa ttaattatgt tacttgatga
     4441 tctttagatt ctgatttgtt tgtttgaaat cccttgtgat tttcagatcc tgtgtatatg
     4501 ggccgacatc atgattctgg gtatgatcca tatacggttt tgaatattca gcgtcgtaat
     4561 gatttttgag ttgagtctac ttgagataga ccttttggtg ttgtaaaatt tgttccaaaa
     4621 tatcatgtta catgatatac ggtagatttt ggaatggtgt atatatcttg aagtaattgg
     4681 acaacggaaa caacattatc ttttgagtga gcagatatta tcaatccaag ccagcaagcc
     4741 ttgcgtttaa cagggactat tgaggttgaa cagaaacctt gtactcttga tacagttgcc
     4801 cctattgtgt cagataaaaa ttatgtaaat tgatccacta gaaatataaa ttccggacta
     4861 aatgtagagt taactgattc aaatggaact gaggttcctt atgtgtggag gaatggagcg
     4921 tggacatgaa acatttgggg catcaataat caatactgag tggatacctc aactataaat
     4981 gtaaatgttt cttgaaatgg ttattctgtt actttatata gcaataattt tcatattaac
     5041 ccattgggta cagggaaaac ttggcaagat ttagatagag attataaaat aactttgagc
     5101 tcttgagagc tttttgattt ttgaatagag aaattaatta ctattaaata tatagtagaa
     5161 gatcgtgcat gaaataaagc aaacgctata cacttattta atcaacctaa aaacccgtgg
     5221 gtaagtgaag tttctccaag taataatcag tctgatgttg catcaaattc tgtaattaga
     5281 ttcaatgtac atgatgatcg agcttgagtg gattcgggaa caattaatct tgtacttagt
     5341 tgaagatgaa atacatttta tgctaactac tcttatcttg attttgagtt gacacctatt
     5401 ggttgaagtg cgaattttga tgattacaat gttgtgcttc aatctcatgc gccattccct
     5461 tcatcatgaa taatagctat gagcgtatct tgatatgatt tagcttgaaa tggaggcttg
     5521 ctttatagct gacaattttt taccaaagct agttgtgagg attttggctg tagttcaaca
     5581 aatatggtta aaattagtac ttgaaattta ttgaatttca taaattatta tcataatgta
     5641 ctatatattt ctggaggtgt aaacccatct cttattcgcg atggtgggaa tacgggatat
     5701 ataaattgta acttacaaaa caatttctta tccatatatg gttgatgagt gggttgaaca
     5761 tgatgggaat ttcgctgaaa gtctgctttt gatgaattac aaataatatg agatgatgta
     5821 atagcaacat tgagtggaga tgttttgact ttggaaagaa taaatgtata tactcctccg
     5881 ccaataacaa cagataatat tgatggtgaa cggataaaaa ctgatttttt ggtagaatta
     5941 atcgcagaag ctacatcatg acaatgagtg gctaatactt attattgtac ttgaattgac
     6001 acaagttgta caccatatat catttgaacg ccacctgtta caacatttac agtaacttgt
     6061 ccaaacaata caacttgtcc atcacaatat gtga
//
LOCUS       DAOSID010000036         5980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_11966_length_5980_cov_15.039325, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000036 DAOSID010000000
VERSION     DAOSID010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11966_length_5980_cov_15.039325"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            511..2520
                     /locus_tag="PK674_02255"
     CDS             511..2520
                     /locus_tag="PK674_02255"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOG15386.1"
                     /translation="MKKQFKLIFFLLSIFFVFGFSFIYAGARNLPGIKIITRDQRGAN
                     EALRYSDMSKTERSKVNAEKNEQKMNELRETDAEKYWSIKEKEYQEQMANDYLKSNYY
                     SEIDLDWTKYESNGNYLFWPEYIKNNKTKIIVHHTASDNTILKNKADVLEYLSGVYRY
                     HTVTNGRGDIGYNFLIDPFGNIYEGRAGGEGVVGAHAKRNNTPSIGISLIGNFENIKP
                     TKEALESLIRLSAALAKKYNINPFSRTDYHKDSKEVPYIVSAENYVIAGHKDAGITAC
                     PGKYLYELLPFVRNGVKDLLEGKTLTSASGLKSVKETITSKQTPKTKRTRLTYDYFES
                     MQSKIAPAVRAIKNDYINKNNITFSKGNISRLVGKIDKNQAKTYLNQDILVLLYELTQ
                     DYSEYNLSCEQGCIFYFNQNKLEANNATIKVSDKLQLNLGGNTYNVDRLSVVPKNSLI
                     TIDNYTRKSYAGIAWNTFRGSLIFKKDYMKDLNGVEQNKYVVINKLPFGDYMKGIAET
                     NDTESATKNEVMSLISKSYALFYMNPKNIHPNIPSQATYNAVDDSRIFQKYVGAGLEK
                     TLTKWYTALEKTKDKIILYNNNVPLLPYFSCSAGFTYSANEKRGRNDTPYLKSKFDLG
                     ICQDKKFSGHGVGLSGLGAERWTQFGWSYTDILHYYYPGIVIETL"
     gene            2607..3434
                     /locus_tag="PK674_02260"
     CDS             2607..3434
                     /locus_tag="PK674_02260"
                     /inference="COORDINATES: protein motif:HMM:NF015748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOG15387.1"
                     /translation="MEEILQKIFPNSAEKAVDSVLNLYEKQGFVISNFIYFAIIKEQK
                     LFESTKKTTLQKDYYKAITKGDFLLPDGIALQTFYRLANRRFSLPTKNLSNLNGTDFV
                     PYFLDEIKKRYGSQRISISLYGAKKEIVQEVKNTFSYKGFEVSYIQDGYSEFDRESFK
                     KSKNNNEEVLNILLVARSTPTNPIQELRTMKNYYKIKESNLIVFTVGGLFDFLAGESP
                     NSSVKKGTQKRAPKFVRKIKLEWLWRFITDPKRNYKKVKNSFSLFPYIFRYLLLKKD"
     gene            3504..5576
                     /locus_tag="PK674_02265"
     CDS             3504..5576
                     /locus_tag="PK674_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15388.1"
                     /translation="MQIKEKKHNYLADNQSFSFKEKLANGVIINAYVVFDSDKFLEYQ
                     KDLLQDFLAEFIDKSNTDIYDETDLKNNLEDGLQSLNTNLRKFSDKVTDVDRFTIKGY
                     IQIIAGTTIMASMIGDISFIMFRNKRLNYQLHNGFNKKSKIDIFSDFIEGDIQTGDEL
                     LYIGTKLSDVLDQSDINEMENILSAPETSFIEFIEKLVTARVEKENIALMSNYHIYGN
                     VIEKQSTSRLPKIKNPIKINTAWTKRILANKYYITILVLSIVILVMLYSVLSQLLNTS
                     KDSVFKTSDGVIVDITIDDIKKDILLFKQMDPSSDQKGVKYSQIIEKLNLLESKGRWL
                     EDVENLKGIIEDDYYKGFNIIRLESLSKLDDPATGIKTRVLSLNNTEKTKVGDVLFID
                     FQRGLNIGGTKGAMIGVVNDGSRGSLVEFNIDSDIQGCSSNLLRDGLYCYTKDGRIFS
                     VNKAGVEPLITSDPAGFPDTIGGVGVYGKANLYVFQSNLNNSRDGAFVTRYRNIVGSQ
                     VSYQGGENYSLMAGYESGVSFSGDFGDVAIDSTFLTWNAGKLYQLRRPGYGVSLDVRE
                     IKLLGGDTITNKYSDDIKVIAYLNSKYVYLFDRINKTFTIYTSNPLKTNDQYNTQFKL
                     YYLFSFKFDLSNEKVLDISISENSGNKPEMYILTNEGVNKVNLYEYIDSLVNDDTLKA
                     IGGGEAPL"
     gene            complement(5728..>5980)
                     /locus_tag="PK674_02270"
     CDS             complement(5728..>5980)
                     /locus_tag="PK674_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15389.1"
                     /translation="KSAVEDDLSAISETSGKGTCCFEVQNPLRFFLNVWESIKSGFSA
                     EINKVYEKEGLVKFGNKIPPLGEVAIPAPPRSTAGLRAG"
BASE COUNT         2249 a          878 c          952 g         1901 t
ORIGIN      
        1 ttttttcaaa aaaagattca atgaaaaata aaaatcattt aatttattta taatagtcca
       61 tttattgcgg ggctacgggt ctacttacat tttgtaataa atatgtaata aagaactccg
      121 cataattctg cggtggaccg ttctctacag aatatcattt cttctatcac ccaggttgta
      181 aataaaaaag ttcttccctc gggggaagtt gggcacccgc cccgcccgca gtattgcggg
      241 gctacggggc ggggtagatg ggggtttgta gttgggggtt agattccctt agtccccctt
      301 gtctacggag tcctccgtaa cgagtaaggg ggattttacc tctaactagc tatgcaaacc
      361 gccccaaggg agaagctacc aactttacaa acttgtaact ttacaatttt tcaagcttta
      421 caaacttctt ttactattag ttgaaaataa aaacttattc aatataaaaa agaggataga
      481 ttttttacaa atataaaaaa taccattatt atgaaaaaac aatttaaatt aatttttttc
      541 ttgctatcaa ttttttttgt attttgattt tcatttattt acgcaggagc acgaaactta
      601 ccatgaatca aaatcatcac tcgtgatcaa cgatgagcaa acgaagcttt gagatattct
      661 gatatgtcaa agacagaaag aagtaaagta aatgccgaaa aaaatgagca aaaaatgaat
      721 gaactcagag aaacagatgc agaaaagtat tggtcaatca aagaaaaaga gtaccaagaa
      781 caaatggcaa atgattattt gaaatcaaat tattattccg aaatagattt ggattggacc
      841 aaatacgaaa gcaatgggaa ttatcttttt tggccagaat atatcaaaaa caataaaact
      901 aaaattattg tacaccatac agcatcagac aatacaattc taaaaaataa agctgatgtt
      961 ttggaatatc ttagtggagt ttatcgatat catactgtta ctaattgacg aggggatatc
     1021 ggttacaatt ttttgattga tccatttggg aatatttatg agggtagagc aggatgagag
     1081 tgagtagttt gagcacatgc aaaacgaaac aatactccta gtatcggaat ttcattaatt
     1141 tgaaattttg aaaatataaa accaacaaaa gaggcattgg agtctcttat tagattgtca
     1201 gcagctttgg caaaaaagta caatataaat cctttttcta gaacagatta tcataaagat
     1261 tccaaagaag ttccgtacat agtttccgct gaaaattatg tgatagcatg acacaaagat
     1321 gctggtatta ctgcatgtcc tggtaagtat ttgtatgaat tgcttccttt tgttagaaat
     1381 ggagtaaaag atcttttgga atgaaaaact ctaacaagtg ctagtggttt gaaatcagta
     1441 aaagagacta ttacaagcaa acaaaccccc aaaactaaaa gaactagact tacctacgac
     1501 tattttgaat ctatgcaaag taaaattgcg cctgcagtta gagccattaa aaacgattat
     1561 atcaacaaaa ataatattac attttccaaa gggaatatta gtagattagt atgaaaaata
     1621 gacaaaaacc aagctaaaac ctacttaaat caggacattc ttgttctgct ttacgaacta
     1681 actcaagatt attctgaata taatttgtct tgtgagcaat gatgtatttt ttattttaat
     1741 caaaataaac ttgaagcaaa taatgctact ataaaagttt ctgacaaact acagttgaat
     1801 ttgtgatgaa atacttacaa tgtagataga ttgtctgttg taccaaaaaa cagtcttatt
     1861 actatagaca attacactag aaaatcttat gctggtatag cttggaatac ttttcgtggt
     1921 tctttgattt tcaaaaaaga ttatatgaaa gatctaaatt gagtagaaca aaataaatat
     1981 gtagttataa acaaacttcc tttttgagat tatatgaagt gaatagcaga aaccaatgat
     2041 acagaatcag ctacaaaaaa tgaagttatg tcgttgatat ccaaatctta tgctttattt
     2101 tatatgaatc caaaaaatat tcatccaaat ataccttcac aagctaccta caatgctgtg
     2161 gatgattcta ggattttcca aaaatatgtg tgagcatgat tagaaaaaac tttgacaaaa
     2221 tggtatacag ccctagaaaa aacaaaagat aaaattattc tttacaacaa taatgttcca
     2281 ttgttaccat acttttcatg ttcagctgga tttacttatt cagcaaacga aaaacgatga
     2341 cgaaatgata ctccttatct aaaatcaaaa tttgatttgt gaatatgcca agataaaaaa
     2401 ttctctggac attgagtcgg actaagctga cttggagctg aaagatggac tcaattcgga
     2461 tggagttata cagatatact tcactactac tatcctggaa tagtgattga aaccctttag
     2521 ttgccgtctt caacaatgtt ttttacaaga atatttaaaa tataatcttg tcaaaaataa
     2581 aaacatttta ttactaaata taacacatgg aagaaatttt acaaaaaata tttcctaaca
     2641 gtgctgaaaa ggctgttgat agtgtcttga atttatacga aaaacaatga tttgtaatat
     2701 caaattttat ttattttgct attatcaaag aacaaaaact ttttgaatcc acaaaaaaaa
     2761 ctactcttca aaaagattat tacaaagcca tcacaaaatg agattttctt ttaccagatg
     2821 gtatagcttt acaaacattt tatcgtttag caaaccgtag attttctcta ccaacaaaaa
     2881 atctttcaaa cttaaatggt acagattttg tcccttattt tttagatgaa atcaaaaaaa
     2941 gatacggtag tcaaagaatt tctatatcat tatattgagc aaaaaaagaa atagttcaag
     3001 aagtaaaaaa cactttttct tacaaatgat ttgaagtttc ttatattcaa gattgatatt
     3061 cagaatttga tcgagagagt ttcaaaaagt ctaaaaataa taatgaagaa gttctaaaca
     3121 ttttgttagt agctagatct actccaacaa acccaattca agaattgcga actatgaaaa
     3181 attattataa aatcaaagaa agtaatttga tagttttcac agtatgatga ctattcgatt
     3241 tcttagcatg agaaagccca aatagttcag taaaaaaatg aacacaaaaa agagctccaa
     3301 aatttgtcag aaagataaaa ttagagtggc tttggaggtt tattacagat cccaagagaa
     3361 actacaaaaa agtaaaaaat agtttttcat tatttcctta tatttttaga tatttactat
     3421 tgaaaaagga ttaaattttg ataccttaaa aaagtaaaac atttttgtga atacaatttt
     3481 ttatatgatt tttccccaaa gatatgcaaa ttaaagaaaa aaaacataat tatttagcag
     3541 acaatcagtc tttttctttc aaagaaaaat tagcaaattg agtaatcata aatgcttatg
     3601 ttgttttcga ttcagataaa tttttggaat accaaaaaga tctattacaa gattttttgg
     3661 cagaatttat agacaagtca aatactgata tttatgatga aacagatctc aaaaacaatt
     3721 tagaagatgg ccttcaatcc ctaaatacta atcttagaaa gttttctgat aaagttacag
     3781 atgtagatcg ttttaccatc aaatgataca ttcaaattat agcaggcacc actattatgg
     3841 cttctatgat atgagatatc tctttcatta tgtttagaaa caaaagatta aattaccagc
     3901 tacacaattg attcaataaa aaatctaaaa tagatatatt ttcagatttt atagaatgag
     3961 acatccaaac ttgagatgag ttattgtaca ttgggaccaa attatcagat gttttggatc
     4021 agtcagatat caatgaaatg gaaaatatcc tttcagctcc agaaacttca tttatagaat
     4081 ttatagaaaa acttgttact gctagagtag aaaaagagaa tatagctctg atgtccaatt
     4141 atcatatcta tggaaatgtt atagaaaaac aaagcaccag taggttacca aaaatcaaaa
     4201 atcctataaa aattaataca gcttggacca aaagaatact agcaaataaa tattatatta
     4261 cgattttagt tttgagtatc gttattttgg tcatgctata tagtgtttta tctcagctat
     4321 taaatacttc caaagattct gtatttaaga catcagattg agtgattgtg gatattacaa
     4381 tagatgatat caaaaaagat attttacttt tcaagcaaat ggatccaagt tcagatcaaa
     4441 aatgagtaaa atatagtcaa ataatagaga aattaaactt attagaaagt aaaggaagat
     4501 ggctcgaaga tgttgaaaat ttgaagggaa ttatagaaga tgattattac aaatgattca
     4561 atattattag attggaaagt ttatcaaaac tggacgatcc agccactggc ataaaaacta
     4621 gagtattatc attaaataat acagaaaaaa ctaaagtcgg tgatgttttg ttcattgatt
     4681 ttcaaagatg attaaatatt tgagggacta aatgagctat gatatgagtg gtaaatgacg
     4741 gttctagatg atctttagta gaatttaata ttgattctga tattcaatga tgttcaagca
     4801 acctattaag agatggtttg tattgttata caaaagatgg tcgtatcttt tctgtaaata
     4861 aagctggagt ggaaccactt attacttcag atccagcggg attcccagac acaatttgat
     4921 gagtaggagt atattgaaaa gcaaacttat atgtatttca atccaactta aacaattccc
     4981 gtgattgagc atttgttact agatatagaa atattgtatg atcccaagtt tcttaccaat
     5041 gatgagaaaa ctattctctt atggctggct acgaaagttg agttagtttt tcttgagatt
     5101 ttggagatgt cgcaatagat tctactttcc ttacttggaa tgcttgaaaa ttatatcagc
     5161 tacgaagacc tggttatgga gtttctttgg atgttcgtga aattaaatta ttatgatgag
     5221 acacaattac aaacaaatat tccgatgaca tcaaagttat tgcataccta aactccaagt
     5281 atgtctattt atttgatagg ataaataaaa catttacaat atacacatcc aatcctctaa
     5341 aaacaaacga tcagtacaat actcaattca agctatatta cttatttagt ttcaagtttg
     5401 atttatctaa tgagaaggtt ctggatatat caatatctga aaattcttga aataagcctg
     5461 aaatgtatat tttgactaat gaatgagtaa ataaagttaa cttatacgaa tatatagata
     5521 gtttagtaaa cgatgatact ctaaaagcta tctgatgatg agaggcccca ctttagttat
     5581 aagttacaag ttaaaagcct gccccgccac gtagccccgc aatactgcgg ggcggggttt
     5641 gtaaagttac aagttgaaaa tccttccctt gggggaagtt gagcgcgaag ctagatgggg
     5701 gttgtagttt aggggtaata cccccaccta ccccgcccgt agccccgcag tactgcgggg
     5761 cggggcgggg attgcaacct cccccagggg aggaatctta tttccgaatt ttaccaaacc
     5821 ctccttttca taaactttat ttatttcagc actaaatcct gattttatgg attcccaaac
     5881 attaagaaaa aaacgaagcg gattttggac ttcaaaacag catgttcatt tgccagaagt
     5941 ttcacttatt gcagataaat catcttctac tgcacttttt
//
LOCUS       DAOSID010000037         5874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_12270_length_5874_cov_13.919918, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000037 DAOSID010000000
VERSION     DAOSID010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12270_length_5874_cov_13.919918"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..609)
                     /locus_tag="PK674_02275"
     CDS             complement(175..609)
                     /locus_tag="PK674_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOG15390.1"
                     /translation="MKRGVDYIGVGCGCLIVNNDHQVLLIKRTSKSQGGGGGFWAQPG
                     GAIEFGDTLEDTIKREIKEELDVDIELIGPQIFAEDIREENGIKKHRFTGGCFAKIIG
                     GNIKIMEPEKCSEAKWFDLDNLPEPIIGYTKPFIEKYKERIK"
     gene            complement(817..1605)
                     /locus_tag="PK674_02280"
     CDS             complement(817..1605)
                     /locus_tag="PK674_02280"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="TatD family hydrolase"
                     /protein_id="HOG15391.1"
                     /translation="MDFEKIGGQALVNIGADEEYNKNGITISKEYSGDSFIKCTVGLH
                     PYEVVVGNINENNIEQKLNELEKLYLENKDHIVAIGEAGIDTHYEGDINLELQKELFK
                     KQCDLAEKIQAPIIIHSRNDFDSTIEILQNYKKLKICFHCRGYGSEEIEKLKNYNLEN
                     YWIGFCGNVTYPKAQNIRDSLKICNLENILIETDAPYLSPQELRGQTNYPQNIQYIYD
                     FIAKELNIDTENLKEQIKKNFYSLYKKYHKVNHGENEFTKRGNT"
     gene            complement(1713..2195)
                     /locus_tag="PK674_02285"
     CDS             complement(1713..2195)
                     /locus_tag="PK674_02285"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOG15392.1"
                     /translation="MKKIKSKKLKKSLIVLIILLIIYIILRFMFKFIIGVNKVCYAKG
                     GFYNKCFIVEVAKTDKERQKGLMDRENLKKNRGMIFVFEEEKNYSFWMKDTLISLDII
                     RIDSQGKIVDIQTANPCDATLCPNYVPQGNAKYVLEINAGIAEKKGIKIGDTLTFKLK
                     "
     gene            2262..2870
                     /locus_tag="PK674_02290"
     CDS             2262..2870
                     /locus_tag="PK674_02290"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HOG15393.1"
                     /translation="MKFLKILLRILILIVIIFVLFFTWNWLQREAVIVTSSTKSVVAK
                     LQSVNKLESAEMTITKIMRAEKEMIDIIPTMSFDNTIQDFLFQDKMIFEVEGKVIAGI
                     DLSKVQTGDVFTKIDGSVKINLPEAEVLHVILHENSKPFDRKIGVFTKGNLETETKIR
                     NKAKEEMEVEAIESGILELAQKNAKENLEKIFEDLDIRVDLD"
     gene            3073..3762
                     /locus_tag="PK674_02295"
     CDS             3073..3762
                     /locus_tag="PK674_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="CBS domain-containing protein"
                     /protein_id="HOG15394.1"
                     /translation="MKNQKLNRECDKNSNLIMEFLALFNKIDKHFDKLLGIDKFIPFN
                     EKIKRIIKGDYPISWFVKLNQYQIKHFGEIRNELTHGIKLDGYSYLYPSDYAISQLKK
                     YVDVIKAPFRCTDLFKKPVFTCKIHDKLTKVLKVMHKNNHSHVPVYDENKNYVGLLAE
                     SEILAWLIEGESAKDITTAKVGDVSLISDLEYIVFVDKKCNVYEIDKLFALKKQKKKK
                     TWSDFNHRKLR"
     gene            3925..4491
                     /gene="rsmG"
                     /locus_tag="PK674_02300"
     CDS             3925..4491
                     /gene="rsmG"
                     /locus_tag="PK674_02300"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOG15395.1"
                     /translation="MWDNLIKIFIEKNNQLNLSAIRDVEGIKIKHINDSIELNKIFQI
                     EKGKTVCDIGTGGGFPLLPLAIINPNVNFIGIDARRKKVDAVNDMIKNLGLKNVVCKR
                     SRIEEYGEKFDFVTARAVAYIDKLIPRSYNLLKKGGYFVFYKQYQQAEHEELKKICKK
                     YKLNIKEKHKYFLFAGDIERVIYIVEKI"
     gene            complement(4492..5607)
                     /locus_tag="PK674_02305"
     CDS             complement(4492..5607)
                     /locus_tag="PK674_02305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="glycosyltransferase"
                     /protein_id="HOG15396.1"
                     /translation="MRIAFVNCRIMPGGVLNVFEDLIESEDFSETKIFTLFSDRKFLE
                     TKKGNIEIVTALPKRINKLFLKFSKKKGFLASILDYRNLMFFYIPLMKILGRKIKKYN
                     PRKVVISSFAVAKNLDFCKSDYNGNFKPQTTLYLHSPMQYIWSHYQEYSQKIRGYKIK
                     IFKAIVPKLRKWDKKFTKYDKVFTNSEYTANLAKEIYKIDSKIKYPKIDNSFFNITAN
                     NNPDNYYIYVGRLVKFVKELDKIIHLFNETQETLLIMGSGPDEEYLKSIANGNIIFIG
                     RIQDPIEKSKIIQNSKGLINITKESFGICTVEALLSGIPVFAYNDGGSLELIDKDSGI
                     LVPNKKHETLLKYFSQFTKKDRNRKKIQDNIKNKLKQ"
BASE COUNT         2090 a          715 c          801 g         2268 t
ORIGIN      
        1 atgaaactgt aaaaaactat aatacaaaat taagaacttt ccctactaat attatcgctg
       61 gattgtttgg ttttgaaaaa gcaaatcttt ttgaagcagc agaaggaagt gaagtagctc
      121 ctgtagtaga gttttaattt ttttaattaa gtttttaact attgtttgac tatattattt
      181 tattcgttct ttatattttt ctataaatgg ttttgtgtat caaataattg gttcaggtaa
      241 attatctaaa tcaaaccatt tagcttcact gcacttttct ggttccataa tttttatatt
      301 tcctcaaatt attttagcaa aacaaccacc agtaaaccga tgttttttta ttccgttttc
      361 ttctctaata tcttctgcaa atatttgggg tcatattaat tctatatcta catccaattc
      421 ttcttttatt tctcttttta ttgtgtcttc caaggtgtca ccaaactcga tagccccgcc
      481 aggttgcgcc caaaatcaac caccacctca ttgagatttg ctggtccttt taattagtag
      541 cacttgatga tcattgttta caattaagca accacaacct actcaaatat agtctactca
      601 tcttttcatg atgttttata aaatataaat gttataaaat atgaatttag aatggaataa
      661 ctcaaatttt ttaacatatt cccttaagtt ttcttaagtt tagtatagat attttcttta
      721 cttgatcaat aattaaattt aaacttacat tcaggtgtat atcattgaat ttagttaatc
      781 ttttttcata atagcttcga aaactttcta ttcaatttat gtgttacccc tctttgtaaa
      841 ctcattttct caatgattaa ctttatgata ttttttgtat aaagaataaa aatttttttt
      901 aatttgttct tttaggtttt cagtatcaat atttaattct tttgcaataa aatcataaat
      961 atattgaata ttttggggat aattggtttg tcagcgaagt tcttgtggcg acaaataagg
     1021 ggcatcagtc tctattagaa tgttttctaa attacaaatc ttcaaactat ctctgatgtt
     1081 ttgcgctttg ggataagtaa catttccaca aaatcaaatc cagtaatttt ctaaattata
     1141 atttttcaat ttttcaattt cttcacttcc atatcatcgg caatgaaaac atatttttaa
     1201 ttttttatag ttttgtaaaa tttctatagt agaatcaaaa tcatttctag aatgtataat
     1261 tattggagct tggatttttt ctgctaaatc acattgtttt ttgaaaagtt ctttttgtaa
     1321 ttctaaattt atatctcact catagtgagt gtctattcca gcttctcata ttgctacaat
     1381 atgatctttg ttttccaaat ataatttctc taattcattt aatttttgtt caatattgtt
     1441 ttcatttata ttcccgacaa ctacttcata cggatgtagt caaactgtac attttataaa
     1501 gctatctcat gaatattctt tgcttattgt tatcccgttt ttattgtatt cctcatcagc
     1561 tcaaatattt accaaagctt gtcatcatat tttttcaaaa tccaaaatat gcttttttcg
     1621 attttcaaaa agggtggggg agtttagatg agtatgagaa tcaatgaaca tatagaaaac
     1681 taagttcgta aatttgtgga atagcttgga gattatttta atttaaaagt tagagtatct
     1741 cagattttga ttcatttttt ttctgctatt caagcattaa tttccaaaac atatttagca
     1801 tttccctgtg gcacataatt tggacataaa gtagcgtcgc aaggattggc tgtttggatg
     1861 tctactattt taccttgaga gtctattcgt ataatatcaa gtgaaatcag tgtgtctttc
     1921 atccagaaag aatagttttt ttcttcttca aaaacaaaaa tcattcatct gtttttttta
     1981 agattttcac gatccattaa tcatttttgt ctttctttgt ccgtttttgc tacttctaca
     2041 ataaagcatt tgttgtaaaa gcctcattta gcataacaaa ctttgtttac tcaaataata
     2101 aatttaaaca taaaccgtaa aataatgtag ataatcaaaa gaattatcaa tacgataaga
     2161 ctttttttga gctttttaga tttgattttt ttcatgattt tagtagaaaa taaatattaa
     2221 ttgttatttg tatatttatt taaataaaaa aatcaactaa tatgaaattt ctaaaaatct
     2281 tgttgcgtat attgattctt atagtaatta tttttgttct attttttact tggaactggc
     2341 ttcaaagaga agcagttata gttacaagtt cgacaaaatc agttgttgct aaactacagt
     2401 cagtaaataa attagaatca gcagaaatga ctattaccaa aattatgaga gcggaaaaag
     2461 agatgattga cataattcct actatgtctt ttgataatac aatacaagat tttttatttc
     2521 aagataaaat gatatttgag gtagaatgaa aagtaatagc ttgaattgat ttgagtaaag
     2581 tacaaacatg agatgtattc accaaaatag actgaagtgt aaaaataaat ttaccagaag
     2641 ctgaagtgtt acatgtgata ttgcatgaaa atagcaagcc ttttgatagg aaaatagggg
     2701 tatttacgaa atgaaattta gaaacagaaa caaaaattag aaacaaggca aaagaggaga
     2761 tggaagtaga agctatagag tctggtattt tggagctagc acaaaagaat gcaaaagaaa
     2821 atttagaaaa aatatttgaa gatttagata ttagagtaga tcttgattaa accaatatct
     2881 aactgttgtt aatgagtgtt tttgtagaat agtttatcta ttttttgaag attttttttt
     2941 gttatggaaa aaatatttgc gttttcccaa gttgtaaaat taaaatcaac aaataagatg
     3001 tcaaaaattt gcattaaaat acatatttat gtataattgg atatagtttt ataattaaca
     3061 ataaatgttt ttatgaagaa tcaaaaattg aatagagaat gtgataaaaa ttctaattta
     3121 ataatggagt ttttggcctt gttcaacaaa attgataaac attttgataa gttactatga
     3181 atagataaat ttataccttt taatgaaaaa ataaaaagaa taataaaagg agattatcct
     3241 atttcttggt ttgtgaaact aaatcaatat caaataaagc attttggaga gattaggaat
     3301 gaacttacac atggaataaa gttggatgga tattcttatt tgtatcctag tgattatgct
     3361 atttcccagc tgaaaaaata tgtagatgtg ataaaagctc cttttagatg tacagatctt
     3421 tttaaaaaac cagtatttac atgtaaaatt cacgacaaac taacaaaagt tttaaaggtt
     3481 atgcataaaa ataatcattc tcatgtgcct gtctatgatg aaaataaaaa ttatgtctga
     3541 cttttggctg agagtgaaat attggcttgg ttgatagaag gggaatctgc aaaggatatt
     3601 actacagcaa aagttggaga tgtgtccttg atttctgatt tagaatatat agtttttgtg
     3661 gataaaaaat gtaatgtgta tgagatagat aaactttttg ctcttaaaaa acaaaaaaag
     3721 aaaaaaactt ggagtgattt taatcacaga aaattgagat aaaaatgaaa aaattttagg
     3781 gataattaca gctggtgata cagcattgat agatgcttat gtggttcgtt aaagcgattt
     3841 ataccaaata atatttaaaa cataaatagc aattagtaat agctaagtgc taatgagttc
     3901 tttagcctat aactttttaa ttgtatgtgg gataatctga taaaaatatt tatagaaaaa
     3961 aacaatcaac ttaatttatc tgcaattaga gatgtagaat gaattaaaat aaaacatatt
     4021 aatgacagta ttgaattgaa taaaattttt caaatagaaa agtgaaaaac agtctgtgat
     4081 attggaactg gatgaggttt cccacttttg cctttagcta ttataaatcc taatgttaat
     4141 tttatttgaa tagatgctag aagaaaaaag gtagacgcag tcaatgatat gatcaaaaat
     4201 ttgtgattga aaaatgttgt ctgtaagcga tctagaatag aagaatatgg tgaaaagttt
     4261 gattttgtta cagctagagc tgttgcatat atagataaat tgattccacg atcttataat
     4321 ttattgaaaa aatgatgata ttttgttttt tataaacagt atcaacaagc tgaacatgaa
     4381 gaactaaaaa aaatatgtaa aaaatataaa ttgaatataa aagaaaagca caaatacttt
     4441 ttatttgctt gagatataga aagagtgatt tatatagttg aaaaaatata attattgttt
     4501 taatttattt ttaatattat cttgaatctt ttttctattt cgatcttttt ttgtaaattg
     4561 tgaaaaatac tttaacaaag tttcatgttt tttatttgga acaagaattc agctgtcttt
     4621 gtctatcaac tctagtgatc atcaatcatt gtaggcaaat actggaatcc cagacaatag
     4681 agcctctacc gtacaaattc aaaaactttc ttttgtgata tttataagtc atttgctatt
     4741 ttgtattatt tttgattttt ctattggatc ttgaattcgt cctataaaaa taatgtttcc
     4801 atttgctata gatttcagat attcttcatc tggtcaactt cccataatta ggagcgtttc
     4861 ttgagtttca ttaaataagt gaatgatttt atctaattct tttacaaact ttaccaatct
     4921 tccaacataa atataataat tgtctgggtt gttgtttgct gttatattaa aaaaactatt
     4981 gtctattttt ggatacttta ttttggaatc aattttatat atttcttttg ctaagtttgc
     5041 tgtatattca ctatttgtaa acaccttgtc atacttggta aatttcttat cccactttct
     5101 aagttttgga acaattgctt tgaatatttt aattttatat catctaatct tttggctata
     5161 ttcttggtaa tgactccata tatattgcat aggagaatgt agataaagtg tagtttgggg
     5221 cttaaaattc ccattgtaat ctgatttaca aaagtctaaa ttttttgcga cagcaaaaga
     5281 ggaaattaca acctttctag gattatattt tttaattttc ctacctaaaa tcttcatcaa
     5341 agggatataa aaaaacatta aattacgata atctagtatg gatgctagaa atcacttttt
     5401 tttagaaaat tttaaaaata gtttatttat tcgttttggt aatgctgtta caatctctat
     5461 atttcatttt tttgtttcca aaaattttct atctgaaaat aatgtaaaaa ttttagtttc
     5521 actgaaatct tcagattcta ttagatcttc aaaaacattt aaaacacctc atggcataat
     5581 tcgacaattt acaaaagcta ttctcattta taaataacta aatttataaa aattataaga
     5641 cctttaggtt aaagtttgta gattttttta ttttaaattt taaaaactta ctacatttaa
     5701 aactttaatt atatcaactc ttttctattg aaaaaaatat taaaaacaat acaaaaggcc
     5761 tgttgatgtt ttttattttg aaatatattt ttattatgga agaaaaaaat ataaaaattg
     5821 aacaagatga gtatgcagta catacacctc aatctacaaa aattattaca aaaa
//
LOCUS       DAOSID010000038         5845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_12357_length_5845_cov_15.741105, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000038 DAOSID010000000
VERSION     DAOSID010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12357_length_5845_cov_15.741105"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /gene="ruvX"
                     /locus_tag="PK674_02310"
     CDS             complement(<1..341)
                     /gene="ruvX"
                     /locus_tag="PK674_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOG15397.1"
                     /translation="MTVKTQNVLGIDRGSKYIGLAYGIIGQSVFFPIGYLLNDQMIYF
                     NISEIIQRQYIKKIVIGRPSKQKDIQEKIENFIKSLGYIIDKRNITIEKVEEDYTSVQ
                     SGEIVSNFKKNV"
     gene            complement(332..931)
                     /locus_tag="PK674_02315"
     CDS             complement(332..931)
                     /locus_tag="PK674_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15398.1"
                     /translation="MLFLNISSDQVNIEIQNEKLKLDRDKIENKIGPTLVKLYRKYSF
                     DKIFLLNGPGGFTNLRVGTLAINLLNTLEKGGIDIYSISKIDFFKYFINQNILPNKGI
                     IYIGQRKNVRLYDFEKQKYETIKIDNIDKKEDIFFDLVYEKDYFDTETIGIFSKNNNI
                     ILKYKGKEFEISLENLDIKAQKMVEANYFIQPIMGKQGQ"
     gene            complement(977..1666)
                     /locus_tag="PK674_02320"
     CDS             complement(977..1666)
                     /locus_tag="PK674_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15399.1"
                     /translation="MLIFIGIIVFFVCPFNVNAPVNSPADDAVITYNEESRKTMIPSD
                     CTFFFDGCNSCARQENGEIVCHELYCETYKKPRCTDNEIVQYNADSRKEIIAEDCQSF
                     FDGCNNCNKTKNGEIICTLMYCETYAEPVCTDTEGIDNEQVQPNGIGRIWIEQPDIRD
                     QEPKEISREEAKNLILAGKVKSIFQTHSLIVGLTTDEGWFTTIEPIIDEVFEIVEQCG
                     DVCKNIRLATE"
     gene            complement(1730..2032)
                     /locus_tag="PK674_02325"
     CDS             complement(1730..2032)
                     /locus_tag="PK674_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOG15400.1"
                     /translation="MKNKIIDCLKTVYDPEFPMIDLYTLGLIYDIKINKKDKIIDLLM
                     TYTTPSCPMGELLQQLCKNAINEKFPDFVVEIEITFDPMRKVSFIRDKDLQKLFQI"
     gene            complement(2022..2420)
                     /locus_tag="PK674_02330"
     CDS             complement(2022..2420)
                     /locus_tag="PK674_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15401.1"
                     /translation="MSKLNLKFYYWASIAKIVLGLFIAFITYTSINIYEDPAIAVSLG
                     FIGIFIGIRGVSFYLFLWGQKIFRKVENSRILKDSYKLSLLFGIYIIINILLLILGGW
                     TKLRGVLLLVGFILIQTLLLESNHEINNEK"
     gene            complement(2444..2974)
                     /locus_tag="PK674_02335"
     CDS             complement(2444..2974)
                     /locus_tag="PK674_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15402.1"
                     /translation="MKKKLFIAIIFIGVLISGCSDKGNFQFEFDNFYGYFFTEKTFVS
                     DTKLSGLGYNLLKNEILKIYKSDSSSGYTDSIIITKKQSNNSLDDFVKEVIKQTKIKG
                     YKIESTRQIKINCLGENIDGKTINSKFIGNLNTTYFSHSFLKHNNNIYIISYSTQDNK
                     ERKTFSSDVKNIKCKK"
     gene            complement(3384..3644)
                     /locus_tag="PK674_02340"
     CDS             complement(3384..3644)
                     /locus_tag="PK674_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF333 domain-containing protein"
                     /protein_id="HOG15403.1"
                     /translation="MKRKLALMFGILIISILIPACTTSKGKHNNVGLANPASVYCEEN
                     GGTLELVSKPEGIVGICNFPDGSYCEERAYYHGECTVKTNKD"
     gene            3851..4456
                     /locus_tag="PK674_02345"
     CDS             3851..4456
                     /locus_tag="PK674_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15404.1"
                     /translation="MKSTETINKPIIFIDFSRVVSSIGISRHLATCLAQYLTIPKEKI
                     RNMYKKHITLLAKGQFFILDFIKELVPFLKQEYSEKNLIECVDIMPPVSSELLNILKI
                     LKNTYLVYLVSDIYFELGIVVQKNLSPYFDGFIFSFQEGYKKSEPQFWEQIKNKINFE
                     NVEYFIDDKQKNLDLASKYGISGVLCTPDFSLQEILNKIIA"
     gene            complement(4529..5509)
                     /locus_tag="PK674_02350"
     CDS             complement(4529..5509)
                     /locus_tag="PK674_02350"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOG15405.1"
                     /translation="MTKIGLFFGGISSETEISIKSAKNIIKHFDHKKYTLVLIYRHKN
                     GLFYTIKNINKLNEKKILHIHDFKKTFDIALPITHGKYGEDGTLQSIFTIQNIKYCGC
                     HVLSSALCMDKSLIKLFFEGHNIPQTKFINIDFQLMSNTEIENQIQKVKQTLSLPLYI
                     KPSNSGSSIGITKVTKYEQLNKAIQEAKKYDQKVLIEEGLVSPKEIEVGIIGNKKLIV
                     SEPGELILSKDFYDYDDKYKNNQTLIRIPAKIEETQQKKIQNLAKKVYKLCGCSGFAR
                     VDFFISNKKIFLNEINTLPGFTDISMFPMLMNHCGLSYTQLINRIISLAY"
BASE COUNT         1891 a          765 c          792 g         2397 t
ORIGIN      
        1 acattttttt tgaaattgga gactatctcg ccggattgga cacttgtata atcttcttct
       61 actttttcta ttgtaatgtt tcttttgtcg atgatatacc ccaaactttt gataaagttt
      121 tctattttct cttgtatatc tttttgttta ctaggtcgtc aaattacaat ttttttgata
      181 tattgccttt gaattatttc agaaatattg aagtaaatca tttgatcatt caataaatat
      241 caaattggga aaaaaacact ttgtcatatt atgccatagg ctaatcaaat atacttactt
      301 catcgatcta ttcccaaaac attttgagtt tttactgtca ttgttttcac ataattggtt
      361 gaataaaata gtttgcttct accatttttt gagctttgat atctaagttt tctaagctaa
      421 tttcaaattc ttttcattta tatttcaaga ttatattatt gtttttagaa aaaataccga
      481 tagtttctgt atcgaaataa tctttttcat acaccaaatc aaagaatata tcttcttttt
      541 tatctatatt atctattttg attgtttcat atttctgttt ttcaaaatca tacaaccgaa
      601 cattttttct ttgtcagata tatattattc atttgtttgg aagaatgttt tgatttatga
      661 aatacttaaa aaaatctatt ttacttattg aataaatatc aatacctcat ttctccagag
      721 tattcaacaa attgatggcc aatgttcaca ctcttagatt agtaaatcag ccaggtccgt
      781 ttaataaaaa aattttatca aaactatatt ttctgtataa ttttactaga gttggtcaaa
      841 ttttattttc tattttatca cgatctaatt tgagtttttc attttggatt tctatgttta
      901 cttgatcgct agatatgttc aaaaaaagca taaaagattt taggggctaa atacagtttg
      961 tgattattat acaattttac tccgttgcga gacgaatatt tttgcataca tctcagcatt
     1021 gttcaacaat ttcaaaaact tcatcaatta ttggctcaat tgttgtaaac catccttcat
     1081 cggtagttaa tcctactatt agactgtgtg tttgaaaaat agactttact tttcatgcta
     1141 agattagatt ttttgcttct tctcgtgaaa tttcttttgg ttcttgatct ctgatatcgg
     1201 gttgttcaat ccaaattctt catattcagt taggttgtac ttgttcgtta tcgatacctt
     1261 ctgtatctgt acaaactggc tctgcataag tttcacaata cattaaagta cagataattt
     1321 ctcagttttt agtcttattg caattattac atccatcaaa gaaagactga cagtcttcag
     1381 caattatctc ctttcgagag tctgcattat actgtactat ttcattatca gtacatcttg
     1441 gttttttata ggtttcacag tatagttcat gacaaacaat ttctcaattt tcctgtctag
     1501 cacaactatt gcaaccatca aaaaagaatg tacaatctga tggaatcatg gtttttcggc
     1561 tttcttcgtt gtaggttatt acagcatcat ctgctggaga attgactgga gcgtttacat
     1621 tgaaaggaca aacaaagaag acaattattc aaataaaaat taacattagg atagtgactc
     1681 ggatattgct tttgtgtttc ataaatatgg ttgttatata ataaaatttc tatatttgga
     1741 atagtttttg taaatcttta tctcttataa aagacacctt ccgcatagga tcaaaagtga
     1801 tttctatttc taccacaaaa tcagggaatt tttcattgat agcattttta cataattgtt
     1861 gtagtagttc tcacattgga caagatggag tagtataggt catcagcaaa tcaatgattt
     1921 tatctttttt atttattttg atgtcataaa ttagacctaa ggtatataag tctatcatcg
     1981 gaaattctgg gtcataaaca gtttttagac aatctataat tttatttttc attgtttatt
     2041 tcgtgattac tttctagtaa taaagtttgt attaaaataa atcctacgag caataaaact
     2101 catcgaagtt tggtccatca tcagagtatt aataagagaa tgttgataat aatatatatc
     2161 ccaaataata atgagagttt gtagctatct tttagtatgc gagagttttc tactttacga
     2221 aaaatctttt gtccccacaa aaataaataa aaactcactc atcgtattca aataaatatt
     2281 catataaatc ccaagctaac agcgattgct ggatcttcat aaatatttat tgatgtgtaa
     2341 gtgataaaag caataaatag tcctagcact atttttgcta ttgatgccca ataataaaac
     2401 ttgagattta atttgctcat ggagtattat tttttataaa atattatttt ttacatttta
     2461 tgtttttcac atcggatgaa aatgtttttc tttccttgtt atcttgtgtg gaatatgata
     2521 tgatgtatat gttgttattg tgtttcagaa aagaatgaga aaagtaagtt gtattgagat
     2581 ttcatataaa ttttgagttt attgtttttc agtctatatt ttctcacaaa cagtttattt
     2641 ttatttgtct tgttgattct attttatatc atttgatttt tgtttgttta attacttctt
     2701 ttacaaaatc atctagactg ttgttagatt gtttttttgt aataattata ctatcagtat
     2761 atcatgaaga tgagtcgctt ttgtatattt tcaaaatttc attttttagt agattatatc
     2821 ataatcaact taattttgta tcggatacga aagtcttttc ggtaaaaaaa tatcaataaa
     2881 agttatcaaa ctcaaattgg aaattacctt tgtcggaaca tcagcttatt aagactcaga
     2941 taaatatgat ggcaataaat agtttttttt tcatttttta aataaacata taagtgattg
     3001 ataaaacaat tcccacaata attcaaataa taaaagattt tgtataaatt ttaattggat
     3061 tttcgttttg agaccaaata tgagcaacaa gatatcctac cattatcccg caaaatatta
     3121 aatatgctgg catgatgaaa aattttataa tttcatgaag attgggagat aatattgcct
     3181 gtcctctaag aaaaaatagt caaaccatga tagttagaaa aaaaaagaat catttcagta
     3241 tttcaaaaaa ggttttcatt tttatttttt tattattgtt aagtaaatag tggctgagat
     3301 atcgtttgag caatcagcgc ctgttttgtt agttgtattg actttggttt gtatctgaca
     3361 tttatagtat cattgttttc aatctaatct ttattagttt ttactgtgca ttctccgtga
     3421 taataagctc gttcttcaca ataacttcag tctggaaaat tacaaattca aactattcac
     3481 tcgggtttgg aaactaattc caaagttcat ccattttcct cacagtatac agatgcagga
     3541 ttggctaatc agacattgtt gtgttttcct ttgcttgtgg tacaggcggg tataagtata
     3601 cttatgatta aaattcaaaa cataagtgca agctttcttt tcatattata aaaattattt
     3661 aataaataat cttgtttctt accataagac ttttggagaa aaaatcaaac ttattttcct
     3721 taataagtgt ttttttatat attctttttt cttaataagc aatctaaatt ttatataaaa
     3781 cttacaaaaa cattgttttc attagtgtta tttatattac tttgaatgtt tattgttatt
     3841 tttatttgtt atgaaatcta cagaaacaat taataagcca attattttta tagatttttc
     3901 aagagtagtt tcttctattg gtatttcaag acatttggca acttgtttag cccaatatct
     3961 tactattcca aaggagaaaa ttagaaatat gtataaaaaa cacattactt tattggcaaa
     4021 gtgacaattt tttattctag attttataaa agagttagtc ccttttctga aacaagaata
     4081 tagcgagaaa aatttgatag aatgtgtaga tattatgcct ccagtatctt ctgaactttt
     4141 aaatatatta aaaattctaa aaaacacgta tttagtttat ttggtttcag atatttattt
     4201 tgagcttgga atagtggttc aaaaaaatct ttcaccatat tttgatggat ttattttttc
     4261 ttttcaagaa ggatataaaa aatctgaacc tcagttttgg gaacagatta aaaataaaat
     4321 taattttgag aatgtggagt attttattga tgataaacaa aaaaacttag atttagcatc
     4381 taaatattga attagtggtg ttttatgtac tccagatttt tctttacaag aaatactgaa
     4441 caaaattatt gcataaatat caattaatat tgaatataat ttatgattag atcattattt
     4501 tttctcaata aaaaactaac tgataatttt agtaggcaag agaaatgatt ctgttgataa
     4561 gctgagtata agatagccca caatggttca tcaacatagg aaacatggat atgtccgtaa
     4621 atccaggtag cgtatttatt tcgtttaaaa atatcttttt attggatata aaaaaatcta
     4681 ctctagcaaa cccactacag ccacagagtt tgtaaacctt tttagctaaa ttttgtattt
     4741 ttttttgttg tgtttcttct attttagcag gaatgcgaat taaagtttga ttgtttttat
     4801 atttgtcatc ataatcgtag aaatcttttg ataaaattaa ttctcatggt tcagaaacaa
     4861 ttagtttctt atttcctatt atccctacct caatttcttt tggtgagact aatcattcct
     4921 ctataagaac tttttggtca tattttttcg cttcttggat agccttattt aactgttcgt
     4981 attttgtaac tttggtgata cctatagaag atcatgaatt agatggtttg atgtagagag
     5041 gcaaggataa tgtttgtttt actttttgga tttggttttc aatctctgta ttactcataa
     5101 gttggaagtc tatatttata aacttggttt gaggaatatt gtgtcactca aagaaaagtt
     5161 taataagaga cttatccata caaagagctg aacttaagac atgacatcca caatatttaa
     5221 tgttttgtat agtgaaaata ctttgaagtg ttcaatcttc tcaatatttg ccgtgtgtta
     5281 taggtagagc tatgtcaaat gtctttttga aatcatggat atgtaatatt tttttttcat
     5341 ttaatttgtt gatatttttg atggtataaa ataatcagtt tttgtgccga tagataagca
     5401 caagagtata ctttttgtga tcaaaatgtt tgataatatt ttttgcagac tttatagaaa
     5461 tttctgtttc actgcttatt catccaaaaa acaatccgat ttttgtcata ttattttttt
     5521 atcttaataa aattggttta tgttaatttt ttgtgttgtt catttactgt attatgtgag
     5581 ccattatcac catcctattt atttatatgc ttgaggtttg tcttctctat atttatgtta
     5641 attttataga ttcaaatctg tgagatacag ttttagttta ataattcaat ggggtttata
     5701 aatggttttt agaaaaattt ctcaacaaaa aatcgatatc tcatgcggaa agaactataa
     5761 aaatatcgtt tttattaccg ttatttattg ttttttttat ttcttcaata ttttctatgt
     5821 attttcaacc acagttttta gcaaa
//
LOCUS       DAOSID010000039         5809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_12442_length_5809_cov_13.751651, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000039 DAOSID010000000
VERSION     DAOSID010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12442_length_5809_cov_13.751651"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1931)
                     /gene="ligA"
                     /locus_tag="PK674_02355"
     CDS             complement(<1..1931)
                     /gene="ligA"
                     /locus_tag="PK674_02355"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF013790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOG15406.1"
                     /translation="MTLSEQTKILQAYIKENKNISQISQTEISKIYQDLIDCITDHNH
                     IYYIESSPIISDFEYDELFDYLKRIEEYFPFLISGNSPTQKLINQIQEGFQKMEHTNP
                     MLSLENSYNAQNLFEREERLLKILEKAEVSEKPTYYIEPKFDGLGIELIYQDGIFQSA
                     ITRGDGLIGEDVTENVKTIKNLKKILNGNIKGKISFRGEIVIAKSELNKINEERSAKG
                     LQIYANTRNLASGSLKQLDPSITASRNLQCFVYEILYSEQNEILDINNLGLATYPAIL
                     ESKNIHDIVKVCENSETKNKLENEDIDFDGLVIKIKEEELRKLVGSTNHHPRRAVAYK
                     FPAQQVSTQIKSVEFQVGRTGIITPVANVEIVNLSGANISRVSLHNFDFIRERDIHYN
                     DFVRIQRSGEVIPYIVSTIIDRREGNSKKIEAPTNCPSCNTKLTQIDMHYYCQNPNCP
                     AQIKEKIIRFVSKDCMDIEGIGESIVDVLVENNIIGNIADLYKLLDFETERVVRRFPG
                     FADKKVGEIKKQLEESKNKEFRRLLNGLGITGIGKKTAKDIYDELAKQFTHKTRLSDI
                     TKHLTNQEFLIGIYGVGEKIIQGIIEYWHNNQDLLKKLEELGLSFNCYEGNNKTESKG
                     HFCITGSFKVSRPEIIKAM"
     gene            complement(2016..3380)
                     /locus_tag="PK674_02360"
     CDS             complement(2016..3380)
                     /locus_tag="PK674_02360"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HOG15407.1"
                     /translation="MRKKSKDYNIPLRLTRPIIHIGIILVAFYAIYKLRFITDLIPFT
                     QLPIPAINYKETMLFAIIAGLVFVGIGIIKNLYELNKPIQKYFQTYTKVWIYWIITIT
                     FIGYFGTDFIFIYGLSRFIILRGAILSFFLLFLFDQIWNKLESMEHKNGEHKILIIGD
                     NTPKSYDAVSQIKEGFSFKSEFTQLKDLKDINLEGYFIIIAVGIFPKEIIQSIFEKIR
                     FSTTRFYHISEGYFLEDVVYKPENISNLIAMEYKHSKLDGRSIVLKRTFDIICSGIGI
                     ILLFPIFIIIGIIIKLESKGPVFFKQKRVGQHGELFTFIKFRSMVKDADSMKAKLLDK
                     NEREGPLFKITNDPRVTKFGKFLRKTSIDELPQLFCVFIGTMSLIGPRPHLPEEVENY
                     ENRQKRLLSIKPGITGYAQVFGRHNLDFTEEAKLDLYYIQNRSIFMDLYVLLGTFKVV
                     FKGK"
     gene            complement(3395..4351)
                     /locus_tag="PK674_02365"
     CDS             complement(3395..4351)
                     /locus_tag="PK674_02365"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOG15408.1"
                     /translation="MSELKKIIDKTQKVAIFCHENPDGDAIGSMLGLGNLLEKLGKEV
                     FYFSPDKPSKTFSFLKGFEKIKNTFDYGYYNLFIFVDFSEYARIKTIYRGKEKYFSEN
                     QILVFDHHQMLNPPQNRTIISDTESTSTCEIILENTLNIWEEYYDKEIATCLYLGLTT
                     DSGNFRYDKNHERILKNALILIQLGADKKLVIDNFINNKTLGTINFLKTLLNRVNIEG
                     EILYSYYDVDELKDYDIDNEQASYGLTILQEISGPKITMTIRKEDDLVKVSLRSKDTN
                     VEKIAKNFGGGGHIHASGFTQTIKKNFNITKKEIIKKVQTLL"
     gene            complement(4367..>5809)
                     /locus_tag="PK674_02370"
     CDS             complement(4367..>5809)
                     /locus_tag="PK674_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15409.1"
                     /translation="MFLVQNMAGFSLAEADILRRGVGKKKIEIIEQLKIEFTERSAKF
                     RNYKPETAKYIYEKMIEPAASYSFNKSHSVCYAWIAYQTAYLKAYYPVEFYAALIRSV
                     EEDPEEQSKYIYETQNHGIEVLAPNVNESFNHVAAIGGKIRLGFLGIKGIGFDIGEYI
                     QQERQKNGEYKSLEDFLKRCDSVINKKSLEGLIKGGALDQFYNRNTLLQNITSLLERS
                     KSSQTMSMGLFGGDESTQKITFNKIFETSFEENLMMEQEVFNAFVSGHLLDGLYPYIK
                     KGTFLSRLNDEKNTGPYKIVGYISNIQRARKKGYFIEIEDISGKREFFFKDILDFKKF
                     DIVIISGFKSEGKYPRISKLIKTDRDTLIKNASSSFDPEITVTKAKGMRIGESKIELL
                     KEDTMEKDKEIMEQESDLITATVSESQEKIPKETTNIQSIPTFEIPNNLGSIEQIKAI
                     LEQNPGDIVVKVGNITKTISPEGLTQLKSL"
BASE COUNT         1755 a          836 c          772 g         2446 t
ORIGIN      
        1 tccatagctt ttatgatttc tggtcttgag actttgaatg atcatgtaat gcaaaaatgt
       61 cattttgatt ctgttttatt gtttcattcg tagcagttaa agcttaatca aagttcttct
      121 agttttttta gtaaatcttg gttgttgtgc caatattcga ttattcattg aattattttt
      181 tctcaaactc aataaattca gatcaaaaat tcttgatttg tgagatgttt tgttatgtct
      241 gataatcttg ttttgtgtgt aaattgttta gctagttcat cataaatatc ttttgctgtt
      301 ttttttcata ttcaagttat tcctagtcaa ttcaataatc ttcggaattc tttatttttg
      361 gattcttcta attgtttttt tatttcacct acttttttat cagcaaatca agggaatctg
      421 cgcactaccc tttcggtttc aaagtctagt agtttgtaca aatcagcaat atttccaata
      481 atgttatttt ctactagaac atctacaata ctttctcaaa ttcattcaat atccatacaa
      541 tctttggata caaatcggat aattttttct tttatttgag ctggacaatt gggattttga
      601 caataatagt gcatatctat ttgagttagt tttgtgttac aacttggaca atttgtaggt
      661 gcttcaattt ttttagagtt tcactctctt ctatcaatta ttgtagaaac aatataagga
      721 atcacttctc cacttctttg tattcgtaca aaatcattgt aatggatatc tctttctctt
      781 ataaaatcaa aattatgtaa agaaactctg gagatattag ctccacttaa atttactatt
      841 tctacatttg ctactggagt gataattcag gttcttcata cttgaaattc aactgatttt
      901 atctgtgtac tgacttgttg ggctggaaac ttataagcta ctgcccgcct tggatggtga
      961 tttgtagatc aaacaagctt tcttaactct tcttctttta tttttattac aagtcaatca
     1021 aaatcaatat cttcattttc caatttgttt tttgtttctg aattttcaca aactttgact
     1081 atgtcatgaa tgtttttgga ttctaatatt gctggataag tagctaatca taaattattt
     1141 atatccaata tttcattttg ttctgagtat aagatttcat aaacaaagca ttgtaaattt
     1201 cttgatgctg tgatacttgg atccaactgt ttgagacttc cagaagctaa atttcttgtg
     1261 tttgcgtaaa tttgcaatca tttagcactc ctctcttcgt ttattttatt aagttcgctt
     1321 ttagcaatta ctatttctca cctaaaagaa atttttcatt taatatttca atttaatatt
     1381 tttttcaaat ttttaatagt tttgacattt tctgttacat cttctcaaat taatccatct
     1441 cctctggtaa tagctgattg aaaaattcaa tcttgataga tcaattctat accaagtcca
     1501 tcaaattttg gttctatata ataagtaggt ttttctgaaa cttctgcttt ctctaaaatt
     1561 tttagtagcc tttcttctcg ttcaaaaaga ttttgagcat tgtagctatt ttctaaagat
     1621 aacattggat ttgtatgttc cattttttgg aatcattctt gaatttgatt gatcaatttt
     1681 tgtgttggtg agtttccaga aatcaaaaaa ggaaaatatt cttctattct ttttagataa
     1741 tcaaaaagtt catcatattc aaaatccgaa attataggag acgattcgat ataatagatg
     1801 tgattgtgat ctgtgataca gtctattaga tcttgatata tttttgaaat ttctgtttgt
     1861 gaaatctgag aaatgttttt attttctttt atataggctt gtaatatttt tgtttgttct
     1921 gaaagagtca ttatataagt tagaaagttt gtaaagtttc actaccaccc gggtgtaaat
     1981 tagcaattac caataacacc cggtggtagt gtggattact tccctttgaa tacaaccttg
     2041 aatgtcccaa gtagaacata taaatccata aatatacttc gattttgtat gtaatacagg
     2101 tctagttttg cttcttctgt aaaatctaaa ttgtgtcttc caaacacttg ggcatatcca
     2161 gtaattcaag gtttgataga tagtaatctc ttttgtcgat tttcataatt ttctacttct
     2221 tcaggtaagt gaggtctagg tcaaatcaga gacattgtcc ctataaatac acaaaataac
     2281 tgtggtaatt catctatgga agttttgcgt aaaaattttc caaattttgt gactctagga
     2341 tcgtttgtta ttttaaataa tggtcattcc ctttcgttct tgtcaagtaa tttagctttc
     2401 atactatcag catctttgac catagaacgg aactttatga aagtaaaaag ttctccgtgt
     2461 tgtcaaactc ttttttgttt aaaaaatact ggtcatttac tttccagttt tattattatt
     2521 cagattataa taaatattgg aaacaataaa attattcaaa ttcctgaaca aataatatcg
     2581 aaagttcttt ttagaacaat cgatcgtcaa tctagcttgg aatgtttata ttccatggct
     2641 attaggtttg atatattttc tggtttatat actacatctt ccaaaaaata tcattctgat
     2701 atatgataaa atctagttgt agaaaatcta attttttcaa agatactttg tattatttcc
     2761 tttggaaaaa ttcatactgc tattattata aaatatcctt ctaagtttat atcttttaaa
     2821 tctttcaatt gagtaaattc gcttttaaat gaaaatcctt ctttaatttg acttacagca
     2881 tcataacttt ttggggtatt atctcatata attaatattt tatgttctcc atttttgtgc
     2941 tccatgcttt ctaatttatt ccaaatttga tcaaataaaa atagtagaaa aaaagatagt
     3001 atagctcatc gaagtattat aaacctagat aatcaataaa taaaaataaa atcagttcca
     3061 aaatatcata taaatgtgat tgttattatc caataaatcc aaactttagt atatgtttga
     3121 aaatattttt ggatgggctt atttaattcg tatagatttt ttattattca tattcataca
     3181 aaaactaatc cagcgatgat ggcaaaaagc atggtttctt tataatttat tgccggaatt
     3241 ggtaactgtg taaatgggat taaatcggta ataaaacgga gtttgtatat agcataaaat
     3301 gcgaccaaaa ttattcaaat atgaattatt ggcctagtaa gacgcaaagg gatattgtaa
     3361 tctttacttt tttttctcat tttgataaga tttattataa taaagtttga acttttttta
     3421 ttatttcttt ttttgttata ttaaaatttt ttttaattgt ttgtgtaaat catgaagcgt
     3481 gaatatgtca tcctcatcaa aaatttttag ctatcttttc tacatttgtg tcttttgatc
     3541 ttagtgaaac tttgactaaa tcatcttctt tcctaattgt catcgtgatt ttgggtcagc
     3601 ttatttcttg taatattgtc aatcagtatg atgcttgttc attatctatg tcataatctt
     3661 ttaattcatc aacatcataa taactataca aaatttctca ttcaatattt actctattta
     3721 atagggtttt taaaaaatta attgttcaca gggttttgtt attgataaaa ttatctatta
     3781 ctagcttttt gtcagctcat aattgaatga ggatgagtgc atttttcaat atcctttcat
     3841 gatttttgtc ataacggaag ttcccggaat cagtagtaag tcataggtat aggcatgttg
     3901 ctatttcttt atcataatat tcttcccaaa tatttaatgt gttttctaat attatttcac
     3961 aagtgctggt ggattctgta tcacttatta tggtccgatt ttgtggagga ttcagcattt
     4021 ggtggtgatc aaatactaat atttgatttt ctgaaaaata tttttctttt catctataaa
     4081 ttgtttttat gcgagcatat tcagaaaaat ctacaaatat aaacaaattg taataaccgt
     4141 aatcaaaagt gttttttatc ttttcaaatc attttaagaa agaaaatgtt ttgcttggtt
     4201 tgtcaggaga aaaataaaaa acctcttttc atagtttttc tagtaaattc ccaagaccca
     4261 acatagatcc tattgcatct caatctggat tttcatgaca aaaaatagct actttttgag
     4321 ttttatctat aatttttttt aactcactca ttttgccgat gttttattat aaagatttta
     4381 gttgtgttaa tcattcagga gatattgttt tggtaatgtt acctactttt actactatat
     4441 ctcatggatt ttgttccaaa atggctttta tctgttctat acttcaaaga ttatttggta
     4501 tttcaaatgt tgggatagat tggatatttg ttgtttcttt tggtattttt tcttgggatt
     4561 cgcttactgt tgcggttatc aaatcagact cttgctccat tatttcttta tctttttcca
     4621 ttgtatcttc tttcaaaagt tctattttac tttctcatat cctcattcat tttgcttttg
     4681 taactgttat ttctggatca aaactagagc ttgcgttttt gatgagagta tcgcgatctg
     4741 ttttgataag tttgctaatt cttggatatt ttccttctga tttaaatcag gaaataatta
     4801 ctatatcaaa ctttttaaaa tctaaaatat ctttgaaaaa gaactccctt ttgccagaaa
     4861 tatcttctat ctcaataaaa tatcattttt ttcttgctct ttggatattt gatatatatc
     4921 aaactatttt gtatggtcaa gtgttttttt catcgtttaa tcgtgataaa aaagttcatt
     4981 tttttatata tggatacaat cagtctaata aatgtcatga aacaaatgcg ttgaaaacct
     5041 cctgttccat cattaaattc tcttcaaaag atgtttcaaa aatcttgttg aaagtgattt
     5101 tttgtgtact ttcatctcaa ccaaaaagtc acatgctcat tgtttgtgaa gattttgatc
     5161 gttccaataa tgatgtaatg ttttgtaaaa gtgtattacg attgtaaaac tgatctaaag
     5221 ctcatcattt aatcagtcat tctagtgatt ttttattgat tacactatcg catcttttta
     5281 gaaaatcttc taaagatttg tattctcaat ttttttgcct ttcttgttgt atatattctc
     5341 atatatcaaa acctattcat ttaattcata aaaatcataa tctaattttt caacctatgg
     5401 ctgctacatg attgaaagat tcatttacat ttggtgctaa tacttctatt cagtgatttt
     5461 gggtttcata aatatattta ctttgttctt ctggatcttc ttctactgaa cgaattaacg
     5521 cagcataaaa ttctaccgga taataagctt ttagatatgc ggtctggtaa gcaatccagg
     5581 cataacaaac agagtgagat ttattaaatg aataagatgc tgctggttct atcatttttt
     5641 cataaatata ttttgctgtt tctggtttgt agtttctaaa ttttgctgac ctttctgtaa
     5701 actctatttt tagttgttct ataatttcta ttttcttttt tcatactcat cttctcaaaa
     5761 tatccgcttc tgctaaagaa aatcatgcca tattttgaac aagaaacat
//
LOCUS       DAOSID010000040         5805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_12452_length_5805_cov_15.400174, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000040 DAOSID010000000
VERSION     DAOSID010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12452_length_5805_cov_15.400174"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1487)
                     /gene="obgE"
                     /locus_tag="PK674_02375"
     CDS             complement(15..1487)
                     /gene="obgE"
                     /locus_tag="PK674_02375"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="GTPase ObgE"
                     /protein_id="HOG15410.1"
                     /translation="MQFYDEVNICIESGKGGNGLASGRRDAKMAFGGPNGGDGGDGGN
                     IVFVASKDENTLLPYRYKKTFKAQNGEDGKTKDQYGANGESLELVVPVGTLVRDMAGN
                     ILLHLLEDQQREVLLYGGEGGKGNIHFKDSVNQFPNFALLGEPGQKKDIKLELQLLAD
                     VALIGSPSVGKSSLINSVSETKAKVANYPFTTLVPNLGSVQVKSFNFNMIDIPGLIKG
                     AAEGKGLGNAFLRHVLKARIFCLVADLSRFDVGIREIIDIFDEIIEYIKSKIDQKVKI
                     RFFDENEEIIMEVKQNSKIILNKKIVFAINKYDLVSDPEIIEEYKKQFLKNLNIYLRK
                     NFFYEIETESFKKNTFVVSAASHFGLNEWLVGLVKILQKGSLEENTFVINKQNKAKTL
                     KVKMIKDITKIEKNKLLSEGYIEEIDSKFIKIWEINDPEVCKLVFITHWGNEEAEMWF
                     WKEMGLKGFLTEFERNGIRKGDVLKIISYYEGQQDRYILY"
     gene            2136..>2746
                     /locus_tag="PK674_02380"
     CDS             2136..>2746
                     /locus_tag="PK674_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15411.1"
                     /translation="MLKLGDEYFLVLGKEISFSEQYKYEIMEDASSFEIIEKNFLNKK
                     TIELLHWMVYWRYAPYYNVVSKLFLSNEVEKLLDKKQAPKKNKISYKVQINAQTNILQ
                     DLQFSPDGQTLVIFPDLRTLTNLINDEFVSQAGVDVLLSSNSQGKKNKSWWDIKNGTT
                     NIILSTHSEIFQNYKKLKKIIIIRPHKRYYANQQDPRYKTFTVV"
     assembly_gap    2747..2846
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2847..5444
                     /locus_tag="PK674_02385"
     CDS             <2847..5444
                     /locus_tag="PK674_02385"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="AAA family ATPase"
                     /protein_id="HOG15412.1"
                     /translation="ASQLALGQQNSQIEILHLFLSLLDQSDGYIPAILNKIDVPSQTL
                     RSLVVAEISKLPQIQGDYKISVSQDLSKVLLQAESIMKEMGDQYLTTEHLFLAILKGD
                     YPINEILRTQNISYDLVFNAIIEMRNGENIQSQNPENTMDALSKYGKDITQLAEEGKL
                     DPVIGRDDETRRTIQILSRRTKNNPVLIGDPGVGKTAIIELLAQQIVKGDVPDMLKNK
                     KIIEIDMGSLIAGAKYQGEFEERLKAVLKEVEKSDGEIILFIDELHLVVGTGRTQGAM
                     DMGNLLKPALARGQIRVIGATTINEYRQYIEKDAALERRFQPVLVDEPSKDDALAILR
                     GIKKAYETHHGVKISDSAVVASVELSMRYIPDRRLPDKAIDLLDEASASVKMGMTSMP
                     PSIIKLEKKISQLEIEKQAISLETAHLAKGEKIKTESRIQEIEKELSAVKEEYNIKKS
                     ERELDRKLLLEIKEINEQIKQLHHDAEIAEKQTDYNKVAEIKYSQIPSLEKKLEEIEE
                     KMHNAKKEGKIIIKDIVEEDDVATIVSKRTGVPVSRLVQTEIEKLANLEEYLGSRVVG
                     QESAVSSVSNAVRRARAGLKDPNRPIGSFLFLGPTGVGKTELAKQLALFLFNDEKSMV
                     RLDMSEYQEKHTVSRLIGSPPGYVGHEEGGQLTEAVRRKPYSVVLFDEVEKAHPEVFN
                     TLLQLLDDGRLTDSKGRTVDFKNTVVIMTSNIGSDIIMNKLSDKVSSKSNDLKSSPLD
                     LEKDIMPILQSYFRPEFLNRLDDIILFNPVNSEMLSKILEIQLNNVKNLIKSEKNIDL
                     NISQDTKDHIAKVGLDPVFGARPLKRAIQNILLDELALQIIEGKIKNGSRVLVRKEKE
                     KDKLIFECK"
BASE COUNT         2039 a          896 c          878 g         1892 t
ORIGIN      
        1 catttcatcg cttgctaata cagtatgtat ctatcttgtt gtcactcgta atagctgata
       61 attttcaaaa catctcactt tctgattcaa tttctttcaa actctgtgag aaatcatttc
      121 aatcacatct ctttccaaaa ccacatttcg gcttcttcgt ttcaccaatg agttataaaa
      181 accaatttac aaacctcggg atcatttatc tcccaaattt taataaattt agaatctatt
      241 tcttctatat atcattcgga caataatttg tttttttcta tttttgtgat gtcttttatc
      301 atttttactt tcaaggtctt agctttattt tgtttattta tcacaaatgt gttttcttcc
      361 aaagaacctt tttgcaatat ttttacaagt caaaccaacc attcattcaa accaaaatga
      421 gatgcggcag aaaccacaaa agtatttttc ttaaaagatt ctgtttctat ttcataaaaa
      481 aaattctttc tcaaatatat gtttagattt ttcaaaaact gttttttata ttcttctata
      541 atttctggat cagacactaa atcatattta tttatcgcaa aaacaatttt tttgttcaaa
      601 ataattttac tgttttgttt gacttccatg atgatctctt cattttcatc aaaaaatctt
      661 atttttactt tttggtcaat tttacttttt atatattcta taatttcatc aaaaatatct
      721 ataatttccc taattcatac atcaaatctt gataaatccg ctacaagaca aaagattcta
      781 gcttttaata catgcctcaa aaacgcattt caaagtcatt ttcattcagc agctcattta
      841 atcaatcacg gaatatctat catattgaaa ttgaaacttt tgacttgcac acttcccaaa
      901 tttggcacta aagtagtaaa tggataattt gctacctttg ctttggtttc agaaacagag
      961 tttatcaagc tagattttcc aacactagga cttccaatca aagcaacatc agctaaaagc
     1021 tgtaattcta atttgatatc tttcttttgt cacggctctc caagtaaagc aaaatttggg
     1081 aattgattta cagaatcttt gaaatgtata tttcatttcc ctcattctcc tcaatacaaa
     1141 agcacctctc tttgttgatc ttcaagcaaa tgtaacaaaa tattcccggc catatctcta
     1201 acaagagttc agacaggaac gaccaattcc aaactttctc aatttgctca atattgatct
     1261 tttgttttac catcttctcc gttttgtgct ttgaaggttt ttttgtatct gtatggtaaa
     1321 agtgtgtttt catcttttga agcgacaaaa actatatttc aaccatctca accatctcaa
     1381 ccatttggtc atcaaaaagc cattttagcg tctcttctac cagaagccaa accatttcct
     1441 cattttccag attctatgca tatatttact tcatcataaa attgcattca agattttata
     1501 ttgtaaagag tagtaagttc gtaaagcctg tccgaagtac tgcggggata ggtgggggta
     1561 aagtccccct tacttgttac ggagaactcc gtaattaagg gggatctaac ccctaactag
     1621 ctctgcgaac aacttccctc tctcgacaag cctcgaacaa agtgagcttt ctcccgacct
     1681 gtcgggatct caccttgttc gagatctatc gggagaggac ttcgcttcgc aagggaagaa
     1741 tttagtggta aagttcctcc ccttggggag gcaggggttt tgtgcttaac agcttccgac
     1801 aatttcaaac aataacgaac ttgtgcaatc atattaaaaa atgcaaattt tgcaagatag
     1861 aataaaaatg agatttttta gtgcgaaaat aatttttata ataatattct taacccacat
     1921 atattacttg aaagtttgtt gtacaatatt gttgggtgaa gccttttttt agcaaactta
     1981 aaagtattga aaagaaaaaa acttttctta caaaaacaaa agagaattaa ttttattatt
     2041 tatatttttt gattttgact tatttatact gattcactaa atactgttga tctgataagg
     2101 tttggaaagt taatcataaa ttcaatactt gagatttgtt gaaattatga gatgagtatt
     2161 ttttagtttt atgaaaagag attagttttt cagagcagta taaatatgaa atcatggaag
     2221 atgcttcaag ctttgagata atagaaaaaa actttttgaa taaaaaaact atagaacttt
     2281 tacactggat ggtttattgg cgatatgctc cttactacaa tgtcgtttcc aaactatttc
     2341 tttcaaatga agtagaaaaa ttattagaca aaaaacaggc tcccaaaaaa aataaaattt
     2401 cttataaagt tcaaatcaat gctcaaacaa acatattaca agatttacaa ttttcaccag
     2461 actgacaaac tttggtaatt tttccagatt tacgaacctt gactaactta atcaatgatg
     2521 aatttgtttc gcaagcagga gtggatgtac tactttcgtc gaattctcaa ggtaaaaaaa
     2581 acaaatcttg gtgggatata aaaaatggaa ctacaaatat tattctatct acacattcag
     2641 aaatatttca aaattataaa aaactaaaaa aaataatcat tatccgacct cacaaacgat
     2701 attatgctaa tcagcaagat cctagataca aaactttcac agtagtnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnngcta gtcagttagc acttggacag caaaactctc
     2881 agattgagat tttgcatctt tttttatcac tgcttgatca gtctgattga tatatcccgg
     2941 ctattttgaa taaaatagat gttccaagcc aaacattaag atctttagtt gttgcagaaa
     3001 tatcaaaatt accccaaatt caatgagatt acaaaatttc tgtatcacaa gatttaagta
     3061 aagtattatt acaggcagaa agtataatga aagaaatggg ggatcaatat ttgactacag
     3121 aacatttatt cctagccatt ctaaaatgag attatcctat caatgaaata ttaagaaccc
     3181 aaaatataag ttatgattta gtttttaacg caattataga aatgagaaat tgagagaata
     3241 ttcaatcaca aaatccagaa aacactatgg atgctttatc aaagtatggt aaagatatca
     3301 ctcaattagc agaggagtga aagttggacc cagttatttg aagagatgat gaaacaagga
     3361 gaacaatcca gattttatct cgtagaacaa aaaacaatcc cgttttgata tgagatcctg
     3421 gggtttgaaa aacagcaatt atagaacttt tagcacaaca gattgttaaa tgagatgtac
     3481 cagatatgtt gaaaaataaa aaaattattg agattgatat gtgatctttg attgctggtg
     3541 cgaaatatca atgagagttt gaagaacgct taaaagctgt tttgaaagaa gtagaaaaat
     3601 cagactgaga aattattctt tttatagatg aacttcattt ggttgtttga acttgaagaa
     3661 ctcaatgagc tatggatatg ggaaatcttc tcaaacctgc tttagccaga ggacaaatta
     3721 gagttattgg agctactaca atcaacgaat atcgtcaata tatagaaaaa gatgcagctt
     3781 tggaaaggag atttcagcca gtattggttg atgagccaag caaagatgat gcattggcta
     3841 tattgagatg aatcaaaaaa gcttatgaaa ctcatcacgg agtaaaaatt tctgattcag
     3901 cagtagttgc ttcagttgag ctttctatga gatatatccc agatcgtaga ttgccggata
     3961 aggcgattga tttgcttgat gaagcatcag cttctgtaaa aatgtgaatg acaagtatgc
     4021 ctccttctat tattaaacta gaaaagaaga tttcccaatt ggaaattgaa aaacaagcta
     4081 tttctctaga aacagctcat cttgctaagg gcgaaaaaat taaaacagaa tctagaatcc
     4141 aagaaataga aaaagaactt tctgcagtta aagaagaata caatattaaa aaatccgaac
     4201 gagagttgga tagaaagctt ttattggaaa taaaagagat taatgaacaa ataaaacaac
     4261 ttcatcacga tgcagaaata gcagaaaagc agactgatta caacaaagtt gctgaaataa
     4321 aatattctca aatcccttct ttagaaaaaa agttagagga aattgaagaa aagatgcaca
     4381 atgcaaaaaa ggaatgaaaa ataatcataa aagatattgt agaagaggat gatgtagcaa
     4441 ctatagtttc caaacgaaca ggtgttccag tatctagatt agttcaaaca gagatagaaa
     4501 aattagctaa tttggaagaa tatcttggat caagagttgt tggacaggaa tcagcagtat
     4561 catccgtttc caacgcggtt cgtagggcta gagctggtct caaagatccc aatagaccta
     4621 tttgatcttt cctttttctt ggaccaacat gagtaggtaa aacagaatta gcgaaacaat
     4681 tagctctctt cctattcaat gatgaaaaat ctatggtcag actagatatg tcagaatatc
     4741 aggagaaaca tactgtttct agacttatag gttcgcctcc atgatatgtg tgacatgagg
     4801 aaggtggaca acttaccgaa gcagttagga gaaaacctta ttctgtagtt ctttttgatg
     4861 aagttgaaaa agctcatcca gaagttttca acactttatt acagcttttg gatgatggta
     4921 gacttactga ttccaagtga aggactgtag attttaaaaa tacagtagtc attatgactt
     4981 caaatattgg ctctgatatt attatgaaca aactttcaga taaagtatct tcaaagagta
     5041 atgatctaaa aagttctcca cttgatttgg aaaaagatat tatgccaatt ctacaatctt
     5101 actttagacc ggagtttttg aataggttgg atgacatcat tttgttcaat ccagtaaatt
     5161 ctgaaatgtt gtcaaaaata ttagaaatac agttaaataa tgttaaaaat ctaataaaat
     5221 cagagaaaaa catagattta aatatatctc aagatacaaa agatcatatt gcaaaagtct
     5281 gattggatcc tgtatttgga gcaagaccac taaaaagagc aatccaaaac attctcttgg
     5341 atgagttagc attacagatt attgagggga aaataaaaaa tggatctcgt gttcttgttc
     5401 gcaaagaaaa ggagaaagat aaactgattt ttgaatgtaa ataaaaattt gatataaaaa
     5461 aatgaaggga acgaaatttt cgttcccctc ggtcttcaat cttttttcgc aaattaccag
     5521 attacgtttt tgtttttctc attttgcctc ctttcttttt tattttagtt acatctcagt
     5581 ttttttaata aactgaatga ttcatttata gtaaataagg gagtaatttc aagtcttttt
     5641 ttatcccttt tctctcctgt ttaacaagaa aggaatagat tttgataaaa ataaactaat
     5701 tttctcaatg tgaaagtagc tttttaatat ttttatacaa aagctgtatt aaaaggttaa
     5761 ttttcaatca aaaagcacca ttttatagtt aattataggt agtaa
//
LOCUS       DAOSID010000041         5743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_12631_length_5743_cov_12.397152, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000041 DAOSID010000000
VERSION     DAOSID010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12631_length_5743_cov_12.397152"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PK674_02390"
     CDS             <1..401
                     /locus_tag="PK674_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15413.1"
                     /translation="IGKETQELKDFMKTACELNLMGLHADGQTPKNNFDPNDIVTRAE
                     FGTVFSRLLFGDMYNIKDESSVYKNEGYWYKDHLNALKSHGVMTQVDGDRPKYREKRG
                     WVMLMMMRADKFGLFAGKVPALMGIKSLFN"
     gene            1077..1325
                     /locus_tag="PK674_02395"
     CDS             1077..1325
                     /locus_tag="PK674_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15414.1"
                     /translation="MSIVTLGFSDSMETKYENTDMFSLFMKEQGLDDSPKEKRKLIHK
                     CLGFIGTRKDVQQFVSVLGQNKDIDIEDIYGCTFKAID"
     gene            1297..1593
                     /locus_tag="PK674_02400"
     CDS             1297..1593
                     /locus_tag="PK674_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15415.1"
                     /translation="MVALLKLSTNLTTVHTRLIRLTFGKHFFNFQNKQQKQFVDVAKA
                     GFESYRDCGFDFVNFVSRIGGFNGKNSKTQKRRADFIDALFQDFEGRFLILFQK"
     gene            complement(1752..3107)
                     /gene="secY"
                     /locus_tag="PK674_02405"
     CDS             complement(1752..3107)
                     /gene="secY"
                     /locus_tag="PK674_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOG15416.1"
                     /translation="MNKFKKKFKNIIENKSLMNKIFFTLGILAIYRFLVHVPVPFVNI
                     HELMGRIGAVESGGLGYFVMMLGGSLENFALIAIGLAPFINSSIIMQLLGSVVPQLEQ
                     LREQGESGQQKIQQYTRYLTVPLAFLQGIGMVFFINYLLGGNVIDTSIGNLLLAAFIM
                     TVGAILLMRLGELITEKGISNGISILIFSSIVAGITKNVYGNFAGTGNVLGMFIFMIV
                     LILGLIILSIFILKSTKEVPIIYARKGKIQESSILPLPMNPVGMIPIIFSMAFVSFPY
                     LVGKMIVQFQPMNTKLVSMANRVEANLNIYSQQPSMLSIIFYFILIIIFTFFYTLITF
                     SPDRMADDIQKKGGFVPGIRPGQETSKYLNRILMHLCLWGGAGLAFIGIYSYILNYIP
                     FIQNLVQSLGSLPIVVTGSGVIIMVGVVTEIINKVESELIMQKYERYDTDTVNKSLRD
                     L"
     gene            complement(3134..3586)
                     /gene="rplO"
                     /locus_tag="PK674_02410"
     CDS             complement(3134..3586)
                     /gene="rplO"
                     /locus_tag="PK674_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L15"
                     /protein_id="HOG15417.1"
                     /translation="MKLHEIKRSSGLKDKANRIGRGNASKGNYSGKGHKGQKARSGRS
                     VSLFFEGGQTPIIQQMPKARGFKRYYKLIDKYSVINLGELQKDERITNKLELNKFKLK
                     ELGYIRKESDLVKILGNGDFSKEIIFVGLDKFSKSAIEKIEKSNSKIN"
     gene            complement(3611..4327)
                     /locus_tag="PK674_02415"
     CDS             complement(3611..4327)
                     /locus_tag="PK674_02415"
                     /inference="COORDINATES: protein motif:HMM:NF015664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15418.1"
                     /translation="MAERKDKTKKEFEEILLEVRKVTRVTTGGRRMSFRAIILVGDKK
                     GKIGLGLSKGADVTSAVKKASNEAYKNMFNVPITKEKSVPYAVTNKYKACIVRLLPAT
                     QGTGLKAGSSVRSVLDLAGYENILSKIIGSNNKLNNAIATVNALTQYKYQEHFMADNK
                     DEQKENIENDKKEKKDDVEKKENIKNDKKTKVADVKDKKNTKDKAIAKKIVKKEETTK
                     KSTTNKKATTTKTIAKTTKK"
     gene            complement(4363..4722)
                     /locus_tag="PK674_02420"
     CDS             complement(4363..4722)
                     /locus_tag="PK674_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L18"
                     /protein_id="HOG15419.1"
                     /translation="MSHLTNKLKDNIKKYLKRKTRVNTKIKSHKPKYRLIINKSNLYI
                     SAQLVDQSGDIVASINDKKSAGKTKVERAFSAGEELGKKIMSKKLEHIAFDRNGYLYH
                     GRVKAFAEGVRKAGVKL"
     gene            complement(4751..5296)
                     /gene="rplF"
                     /locus_tag="PK674_02425"
     CDS             complement(4751..5296)
                     /gene="rplF"
                     /locus_tag="PK674_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L6"
                     /protein_id="HOG15420.1"
                     /translation="MSKIGKKPINIPSGVEVTIDNNIVTVKGPKGELSEKILDCVSVK
                     KEENNVILSINNIDDGKFRGLSRTLIANMIEGVINGYEKKLLVMGVGYGAQLQGNKLV
                     LSLGFSHKVNYELPNNVKVKVELDPKGNSIITLNSIDKQLLGEIASKIRGFKKPEPYK
                     GKGVRYFDEFVKLKAGKSAGK"
     gene            complement(5328..>5743)
                     /locus_tag="PK674_02430"
     CDS             complement(5328..>5743)
                     /locus_tag="PK674_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=25
                     /product="30S ribosomal protein S8"
                     /protein_id="HOG15421.1"
                     /translation="KLIMSYVNAPIHDLLIRIKNAYMARKTDLDDITFSKFKYNILDL
                     LKRYNFVKNFEVVEKNKKRNIKITLKEVENPVNDIPEIKFFSKPSRPRYVSYKDIKPV
                     AGGKGIGIISTNQGLMATHEAKQKKLGGELIAEIY"
BASE COUNT         1828 a          764 c          797 g         2354 t
ORIGIN      
        1 atataggaaa agaaactcag gaactaaaag actttatgaa aacggcttgt gagttgaatt
       61 tgatgtgatt acatgctgat tgacagactc ctaaaaataa ctttgatcct aatgatatag
      121 tgactagagc ggaatttggt acagtatttt ctaggttgtt atttggtgat atgtacaata
      181 ttaaagatga aagttcggtt tacaaaaatg aatgatattg gtataaagac cacttgaatg
      241 cacttaaatc acattgagta atgactcaag tagattgaga tcgacctaaa tatagggaga
      301 aaagaggatg ggtaatgctt atgatgatga gagcagataa attttgattg tttgcaggaa
      361 aagtgcccgc attaatgtga attaaatcac tatttaacta aaatatataa atcaaacaca
      421 ggaaatgtca acacttgttc tttgtttgat tagttttatg gcttaattac ctatgttttt
      481 gagagtggct tatatttgtt ttcttgaaaa aagcttgaaa attactttga aaatactata
      541 tattcgtatg taaaaagatt gaatgatctt tttaggctca agttagtgag tttaaatacc
      601 acaatcaaac gaactaacaa atcgtagggt gaaaaataac tggaaacagt tatttttctt
      661 ttaataaaaa tattgaaaaa agacataatt aaaaaaaacg ggtggctaaa atgataggaa
      721 acagtagaaa ttaactgctt tatttactgt ttttttataa atgtattata aaatattcct
      781 ttttttaggt gtttggaaga agtatatact actttaagta gtaaggagct tgttttttgt
      841 tttctttttg tgcaaggggt ctttaaaaat ttttctttta tttttaaagg gagattctaa
      901 gatgaggacc tattcttact attgtgtaca ctaataagat gatccaaatg tttaattaaa
      961 ttgatgttca aataaaataa agattttgat tgaatgtatt taacaattag atatatttaa
     1021 caaagaaaaa aaatttttat acttgatagc gtttttttgt tttttattaa aagataatga
     1081 gcattgttac actaggtttc tcagattcta tggaaactaa atatgaaaat acagatatgt
     1141 ttagtttgtt tatgaaagag cagggtttag atgattctcc aaaagaaaaa agaaaactta
     1201 ttcataaatg tttatgattt ataggaactc gaaaagatgt tcaacaattt gtttcggttt
     1261 taggacaaaa taaggatatt gatattgaag atatttatgg ttgcactttt aaagctatcg
     1321 actaatctta ctactgttca tacaaggttg atacggctaa ctttttgaaa acactttttt
     1381 aattttcaga acaagcaaca gaagcaattt gttgatgttg ctaaagcatg atttgaatct
     1441 tatcgtgatt gtggttttga ttttgtcaat tttgtaagta ggattggatg atttaatggg
     1501 aaaaattcaa aaacacaaaa aagaagagct gattttattg atgctctttt tcaggacttt
     1561 gaatgaagat tccttatatt atttcaaaaa taagaaaatt gggaggctca gatgatttaa
     1621 aattttacga gtttgaagat tattgaactt atttagactt cgtttttaga cttcgttata
     1681 gataagtatt ttaaatagag aaaaataaaa aaagactttt aattaagtct ttttttaagt
     1741 aggtatattt tttataaatc tcttagtgat ttatttacag tatctgtatc atatctttca
     1801 tatttctgca ttattaactc tgattctact ttattaatta tttctgtaac aactcatacc
     1861 ataataatta ctccggatcc tgtaacgaca attggtagcg atcatagtga ttgaaccaag
     1921 ttttgtatga aaggtatgta atttaatata taactatata ttcagataaa tgctagtcat
     1981 gctcatcacc acaaacatag gtgcatcaat attctgttta gatatttgga agtttcttgt
     2041 ccaggtctaa ttcacggtac aaatcctcat tttttttgta tatcatcagc cattctgtct
     2101 ggactaaatg tgatgagggt gtaaaaaaat gtgaatatta taattaatat aaaatagaat
     2161 atgatggata acatacttgg ctgttgagaa tatatgttta aatttgcttc tactcggttt
     2221 gccatactta ctaactttgt attcattggt tgaaattgaa caatcatctt accaactaaa
     2281 tatggaaaag atacaaatgc cattgaaaat atgataggta tcattccaac tggattcatt
     2341 ggtagaggca gtatcgaact ttcttggatt ttaccctttc tagcataaat aataggtact
     2401 tcttttgtag attttaatat gaaaattgat aagattatca gccccaatat tagtacaatc
     2461 atgaatataa acattcaaag gacatttcca gttcaagcaa aatttcagta aacatttttt
     2521 gtgattcatg caactattga tgaaaatatt aatattgaga taccatttga tattcatttt
     2581 tctgttatta attctcataa tcgcattaat aaaatagctc aaacggtcat aataaatgca
     2641 gctaacaata aattaccaat tgaggtatca ataacatttc ctcataataa ataatttatg
     2701 aaaaacacca taccaattca ttgtaaaaat gccaatggaa cagttagata tctagtatat
     2761 tgttgtattt tttgttgtcc tgattctcat tgttctctta actgttctag ttgaggaact
     2821 acggatccca ataactgcat tataattgat gagttaataa atggtgctaa tccgattgct
     2881 attaatgcaa agttttctag ggatcatcac agcatcataa caaagtaacc aagtccacca
     2941 gactcaacag ctcagattct tcacattagt tcgtgaatat taacaaaagg tactggtaca
     3001 tggaccaaaa atctatatat tgctaagatt cctaaagtaa aaaatatttt attcatcaat
     3061 gatttgtttt ctataatatt tttaaacttt tttttaaatt tattcataat tttaggtttt
     3121 tactcataaa agattagttt atttttgagt ttgatttctc tattttttct atagcacttt
     3181 ttgaaaactt atccaatcat acaaaaataa tttccttgga aaaatctcaa tttcaaagta
     3241 tctttactaa atcagactct tttctgatat atcataattc ttttaattta aatttattta
     3301 gctctaattt gttggttatt ctttcgtctt tttgtaattc tcataagttt attacagaat
     3361 atttatctat tagcttataa tatcttttga atcctcttgc tttgggcatt tgttgaatta
     3421 taggagtttg tcaaccctca aaaaaaagag aaacggatct gccagatcta gctttttgtc
     3481 ctttatgtcc tttgccagaa taatttcatt tactagcatt tcatcttcaa attctgtttg
     3541 ctttatcttt taggccggag cttcttttta tttcgtgtaa tttcattttt tgtaaaattt
     3601 atataaaata ttattttttt gttgttttag caatagtttt tgttgttgta gcttttttat
     3661 ttgtagttga tttttttgta gtttcctctt ttttaacaat ttttttagca atagctttgt
     3721 cttttgtatt ttttttgtct ttaacatcag ccacttttgt ttttttatcg tttttaatgt
     3781 tttctttttt ttcaacatca tctttttttt cttttttgtc gttttcaatg ttttcttttt
     3841 gttcatcttt attatctgcc ataaaatgtt cttgatattt gtattgtgtt agggcattga
     3901 cagtagctat ggcattattt agcttattat ttgatcatat tattttagat aaaatatttt
     3961 catatccagc caaatccaaa acagacctca cagaagagcc agcctttaat catgttccct
     4021 gtgtagcagg taataatcta acaatacatg ctttatattt attagtaaca gcatatggaa
     4081 cagatttttc ttttgtaata gggacattga acatattttt gtatgcttca tttgatgctt
     4141 tttttactgc agaggtaaca tctgctcatt tggataaacc taatcagatt tttcattttt
     4201 tatctcatac caaaataatt gctctaaacg acattcttct tcatccagtg gttactcttg
     4261 ttacttttct aacttccaaa agaatttctt caaactcttt ttttgtttta tcttttcttt
     4321 cagccattaa atattattac acaaataaat atgttttatt gtttataatt ttactcaagc
     4381 ttttctaact cattctgcaa aagcttttac tctaccatga tacaaatatc catttctatc
     4441 aaaagcaata tgttcaagtt tttttgacat aatttttttt cctagttctt ctccggcaga
     4501 aaaggctctt tctacttttg tttttcaagc tgatttttta tcatttatag aagctacaat
     4561 atccccactt tggtctacta attgtgcaga aatatataag ttagatttgt ttataatgag
     4621 tctgtatttt ggtttgtgag actttatttt tgtgtttact ctagtttttc tttttagata
     4681 ttttttgata ttatctttta gtttgtttgt taagtgagac attttgtaat gttttttaaa
     4741 aataaaatta ctatttacca gcagatttac cggcttttaa tttaacaaat tcatcaaaat
     4801 atctgacccc ttttcattta taaggttctg gttttttgaa tcctctaatt tttgatgcaa
     4861 tttctcctaa taattgtttg tctattgaat taagtgtgat aatagaattt cattttggat
     4921 ctagttcaac ttttactttt acattgtttg gtaactcata atttacttta tgagagaacc
     4981 ccaaagatag cactaattta tttcattgta attgtgctcc atatcaaaca cccattacta
     5041 aaagtttttt ttcgtatcaa tttatcactc cttcaatcat atttgctatc agtgttctag
     5101 aaagtcatcg gaactttcca tcatctatgt tgttgatgga taatataaca ttattttctt
     5161 cttttttaac agaaacacaa tctaaaattt tttcagatag ttctcatttt ggtcatttta
     5221 cagtaactat gttgttgtct atcgtcacct caactcaact tggtatattg atgggttttt
     5281 ttcaaatttt tgacatttta aactgcttat aaataaagta taaagtttta gtatatttct
     5341 gcgattagtt ctcaacctaa ttttttttgt ttggcttcat gtgtagccat tagtcattga
     5401 tttgtagata taattcctat tcctttacct catgctacgg gttttatgtc tttgtaacta
     5461 acatatcggg gtcttgatgg ttttgaaaag aactttattt caggaatatc gtttacaggg
     5521 ttttctactt cttttaaagt tatttttatg tttctttttt tatttttttc gaccacttcg
     5581 aagtttttta caaaattata tctttttagt aaatccaaaa tattatattt aaattttgaa
     5641 aaagttatat catctaaatc ggtttttctt gccatatatg cattttttat tctaatcaac
     5701 agatcatgaa taggagcatt tacataactc ataattaatt ttt
//
LOCUS       DAOSID010000042         5740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_12640_length_5740_cov_9.682322, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000042 DAOSID010000000
VERSION     DAOSID010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12640_length_5740_cov_9.682322"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..2141)
                     /locus_tag="PK674_02435"
     CDS             complement(252..2141)
                     /locus_tag="PK674_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15422.1"
                     /translation="MIYQYLRASSKQVVSKGGTFIEGIFSTTSFLPYLSNDRQSKFYQ
                     GLMFDKCLDFTIDQNGKPEYKNKLCEVTTRDYKTYHVSLTTGSIWSNGVPLSIEDLYF
                     TYNEIIKKNSFNLSYLEKYSNIYVEMEGDKVKVVFENSSEDNSLFFTNYILPQHALLA
                     PNIDMYQQSFAIEPVYNNCAKIKSQSTDQYSLIFDLSDCKDTNLGFYQIKNTISFDNF
                     KEDINQLNGSIIDAYIGDENLKGYQTINLESNRLVTLFFNTKSEKMTVRLRRALGGLI
                     ENKFFDSEKDEYMKKYDRDIFVKYTSTGANISEFLNRINIKGGLGKQELIESGISEFT
                     GNLKFSNKEKAYAFYTEDSKQNFMLNLKFENSYDKIAIQHNSGDLYYPNTYSKKNQSA
                     NYGITPKNKNLNPGLNSYTIFGFNKDKKEQVGSVNLYNLHNDNTNNGVGMKEDLKILY
                     FDNNISNYIIAKLKQIFKEMDIEDRFTYKKSSDINELEGILTAGEYDVFLNIIDMGLT
                     TDMSKLFSVENPKNNPSQYSNARLVSLLKQYNESKNKSKIIEEINNIHSTDMPIVVLG
                     RQYLKLNFKESMLNKLDMEKLNMYEYNRREEIYKNLSLTENIYIDKEKAWNRKNFWEF
                     IKNPN"
     gene            2761..2837
                     /locus_tag="PK674_02440"
     tRNA            2761..2837
                     /locus_tag="PK674_02440"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2795..2797,aa:Cys,seq:gca)
     gene            complement(2939..3637)
                     /locus_tag="PK674_02445"
     CDS             complement(2939..3637)
                     /locus_tag="PK674_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG15423.1"
                     /translation="MRRCGGGGGGGGGGGAGGTLPTPPINTGIVESGNQQLDLLANKL
                     EEESIESQIKKFIEENKNSSPVKSLLNTDFSIELQKAYIFAYMNQITTMPTIDKADLH
                     GNLTRIAMAKMVANYVLSLNLQKPDLDKKCEFSDISSDLNKDYDNGVTKACQLGLMGV
                     GIKEFNPNGIVSRAEFGTVLSRALRGAKYNNDNIYYEKHLEALKKDGIMTKIENPKAQ
                     ELRGRVMLMLMRAK"
     gene            complement(3550..>5740)
                     /locus_tag="PK674_02450"
     CDS             complement(3550..>5740)
                     /locus_tag="PK674_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=25
                     /product="S8 family serine peptidase"
                     /protein_id="HOG15424.1"
                     /translation="IAQNTQHTVSPKYVPGEVLVKYKTSQADISTKRGQNIIKIFAQN
                     KNMEITDNIENYNIVVMKIQGEETVEQMVDNLNNDPNVEYAQPNYIYHTYSTPNDPRF
                     EELRGLSAISWLSGMNIFSGGSDGTGTIIAIIDNGISYNHPDLSGNMRDGSACISGEG
                     TALGDCIHGYDYIENDRDPAPKTGDTHGTHVAGTIGAIMNNNTGIVGVSPNVSLMAIR
                     AGSGIELYDTTIIKGIDFAQQNGATIINASFGFRYYKTGYNDYGILDAIKGFSGLFIA
                     AAGNNGRNNDGNSDSTTYPCSYDLDNIICVAATNSGEELASYSNYGAISVDVGAPGSN
                     ILSTISNDAYGYDGGTSMATPHVVGLASLARSFRPDLSYQEIKNIIINSGDFLDNLSG
                     KTVAGTRINVYQTLYALDNFGPTAPTLIFPLSGEIIFGTDMQFLWSEATDKGVGLSGY
                     YFELSTGIDFQNIITGTSLDTTGITISNLEQNTYYWRVKAFDLKNNTGEYSETGRFFI
                     GFNSILNAEPNTLYTSNIVTLFATGEITISIDTGEYAINSSGWETASGTINSGDTIQL
                     RLTSPNEFNVSNSMTLSYGTNQATFTITTRNKKSDPEPFSFTGITNAELNKLYTSNII
                     IISGLETSHVFTASITAGELIKNNILVSGLSTGVENGDNLSIRLNSSPNYSTTLESTL
                     TVGSGSATFQITTRSKPSSGGGVDEGDEVDEVEVLDELCPLLQLILE"
BASE COUNT         1780 a          888 c          846 g         2226 t
ORIGIN      
        1 tcaacaatct cctcaacccc caactacgat agagcaactt cccccaaggg aagaattttt
       61 ttcacattaa agttcataaa ggtcgtaaag tttataaagt ttataaagtt cgtaaagttt
      121 ataaagtttg taaagcctgc cacgcagtac tgcgtggttc gtcaagtttg taaagttctt
      181 tataacttgt aactttataa actttcaact ttttaacttt caattcgtaa ctttataaac
      241 tagtagttta cttaattagg gttttttata aattcccaaa aattctttcg attccaagct
      301 ttctctttgt ctatatatat gttttctgtc agacttaaat ttttgtaaat ttcttctctt
      361 cggttgtact cgtacatatt caatttttcc atatctagtt tatttaacat ggattcttta
      421 aaatttaatt taagatattg tcttcataaa acaacaattg gcatatcagt agaatgaata
      481 ttgtttattt cttcaataat ttttgactta ttctttgact cattatattg ttttagtaat
      541 gaaactagtc ttgcattgct gtattgagat ggattgtttt ttgggttttc tacggaaaat
      601 aatttagaca tatctgtagt aagtcacatg tctattatat tcaaaaatac atcatattct
      661 ccagcagtca aaattcattc taattcattt atgtctgatg attttttgta tgtaaatcta
      721 tcctcgatat ccatttcttt gaatatttgt ttgagttttg cgataatgta atttgatata
      781 ttgttgtcaa aatataagat tttcaagtct tctttcattc aaactcagtt gtttgtgttg
      841 tcgttgtgta agttgtaaag attaacgctt ccgacttgct cttttttatc tttgttaaat
      901 ccaaaaatgg tgtaagaatt taatccagga tttaaatttt tgttttttgg tgttatccca
      961 tagtttgcag actgattttt tttactgtat gtgtttgggt agtataagtc tcaactattg
     1021 tgttgtatag ctattttgtc gtagctgttt tcaaacttta aatttagcat aaagttttgt
     1081 ttgctatctt ctgtataaaa agcgtaagct ttttctttat tagaaaattt tagatttcat
     1141 gtaaattcac taattcaact ttctataagt tcttgtttcc ccaaacctca ttttatgttt
     1201 atacggttta aaaactccga tatgtttgct catgtagatg tatattttac aaaaatatct
     1261 ctatcatact ttttcatata ttcatctttt tctgaatcaa aaaacttgtt ttcaattagt
     1321 catcataagg ctcttctcaa tcttactgtc attttttctg atttggtgtt gaaaaacaat
     1381 gtgaccaatc tattgctctc aaggtttatg gtttggtatc attttagatt ttcatcacca
     1441 atatatgcat ctatgataga tcaattcaat tgatttatat cttccttgaa attatcaaag
     1501 gaaattgtgt ttttgatttg gtaaaatcat aaatttgtgt ctttgcaatc agataaatca
     1561 aatattaaac tgtattgatc agtggattga gattttattt ttgcacagtt gttgtaaact
     1621 ggttctattg caaaactttg ttgatacatg tctatgtttg gtgccaaaag agcatgttgt
     1681 ggtaaaatgt aatttgtgaa aaataaagaa ttgtcttccg aactattttc aaacactact
     1741 tttactttat ctcattccat ttctacataa atattggaat atttttctaa atatgataag
     1801 ttaaaagagt tttttttaat tatttcatta tatgtaaaat aaaggtcttc tattgataaa
     1861 ggaactcaat ttgaccaaat actcccagta gttaaactaa catgataagt tttgtaatct
     1921 cttgtggtga cttcacaaag tttgtttttg tattctggtt tcccattttg atcaattgta
     1981 aaatccagac atttgtcaaa cataagtcat tgatagaatt tactttgccg gtcattactc
     2041 aggtatggta aaaatgatgt tgtggaaaaa attcattcaa taaaagtacc acccttactt
     2101 accacttgtt tggaagaagc tcgtagatat tggtatatca catataagct ggttaatatc
     2161 cgcaatatta gggctacaaa aaaagtgtac cttaagtatc tttttaaagt atttttttct
     2221 ctaaaggaaa tcattttatt ttacataggg taaaattaaa gaaattataa caaatattat
     2281 acttgttatc agtgctattt ttttaagttt tcattctaat gattttttgc ttccgtattc
     2341 acttcaagct caaatccctc caagtgcgga tcctaaacct ccctttgggg acataagtaa
     2401 gacggctcaa ataaaaatag ctcaactaag caccaacaat atggttaata gagttttcat
     2461 aacataaaga taagacaaaa taaagtatta tactttttaa ctattagcca aaagatttca
     2521 atactatttt aaatccgatc ttattgtgtt ttttgaagtt ttaaatagat tttacaaagc
     2581 aatttgttat ttataaattc cttcgcttga taaaatgttt aaatgatgat atttgtttat
     2641 agtatttact gatagtagtg ctttttgaat tgtaattaat gagataagtt ttgtacttga
     2701 aaacagatag aaaatatgta aaagacaaag agtgggtaat gatgtttttg ataactaagt
     2761 ggcaccctgc tagagtggtc taatagacgg gtctgcaaaa ccttggttca ccggttcgaa
     2821 tccggtgggt gcctccattg ttgttctaat aagaatcaag agaaattact cagttccgtc
     2881 actatcaaaa aaagtctcca atcaaggagc tttttttgtt ttgttaaatt atatggtgtt
     2941 attttgctct catcaacatc aacattactc gacctctaag ttcttgggct ttgggattct
     3001 ctatttttgt cataattcaa tcttttttta gtgcttccaa gtgcttttcg taatatatat
     3061 tatcgttatt atatttagct catcgtaaag ctctggataa tactgttcca aattctgctc
     3121 tgctcactat tccatttgga ttgaactctt tgattcaaac tcccattaag cccaattgac
     3181 aagcctttgt tactccgttg tcataatctt tatttaagtc actagatata tctgaaaatt
     3241 cacatttttt atcaaggtct ggtttttgta agttaaggct taatacataa ttggctacca
     3301 ttttagccat tgctattcta gttaaattac cgtgtaaatc tgctttatct atggttggca
     3361 tagtggttat ctgattcata taagcaaaaa tatatgcttt ttgtagttca atagaaaagt
     3421 ctgtgtttaa aagtgatttt actggactac tatttttatt ttcttctata aattttttta
     3481 tttgagattc tatactttct tcttctagtt tattcgctaa caggtctagt tgttggttcc
     3541 cagattcaac tattccagta ttaattggag gagtgggcaa agttcatcaa gcacctcaac
     3601 ctcatcaacc tcatcaccct catccacacc tcctcatgaa gaaggttttg acctagtagt
     3661 gatttgaaat gtggcacttc cacttcatac tgtcagagta gattcaagtg tagtagaata
     3721 attgggactt gaatttagtc taatggataa gttatctcca ttttctactc cagtactcaa
     3781 tcatgaaact aaaatattat tttttatcaa ttctcacgca gttatactag cggtaaaaac
     3841 atgtgatgtt tccaatcatg atataataat tatgttggat gtataaagtt tatttagttc
     3901 agcatttgta attcctgtaa atgaaaacgg ttctgggtcg gattttttat ttcttgtagt
     3961 gatagtaaaa gtagcttgat ttgttcaata gcttaaagtc atactattgg aaacattaaa
     4021 ttcatttgga ctagtcagtc taagttgaat agtatctcaa gaattaattg ttccgcttgc
     4081 tgtttcccat catgatgaat taattgcata ttctccagta tcaatagaaa ttgttatttc
     4141 accagtagca aagagggtaa ctatattaga agtatagaga gtgtttggtt ctgcatttag
     4201 tatagaatta aaacctataa aaaatcgtca tgtttcacta tattctcaag tattgttttt
     4261 tagatcaaaa gctttaactc tccagtaata agtattttgt tctaaattag atattgtaat
     4321 cccagtagta tccaaacttg ttcaagtgat tatattttgg aaatcaattc atgtagataa
     4381 ttcaaaatag tatcaactta gtcatacacc tttatcggta gcttctgacc aaagaaattg
     4441 catatcagtt caaaaaataa tttctccact taaagggaat atcagtgtag gagcagtagg
     4501 tcaaaaatta tcgagagcat agagtgtttg gtatacatta attcttgtcc ccgcaacagt
     4561 ctttcagcta agattgtcca aaaagtctca actatttata attatatttt ttatctcttg
     4621 ataagacaaa tctggtctaa aacttcgtgc tagtgatgct aatccaacta catgaggtgt
     4681 cgccatagaa gttcagccgt catatcaata tgcgtcatta cttatagtac tcaatatatt
     4741 tgaaccagga gctcaaacat ctaccgaaat tgcaccataa tttgaatacg aagccaactc
     4801 ctcaccacta ttggtagctg ctacacaaat aatattatct aaatcgtaac tacaaggata
     4861 tgtggttgaa tctgaatttc catcattatt gcgtccatta tttcaagctg ctgcaataaa
     4921 taatcatgaa aatcctttaa tagcatcaag aattccataa tcattataac cagttttgta
     4981 atatcggaat caaaaactag cattgataat agtagctcaa ttttgctgag caaaatctat
     5041 tcatttaatg atagtggtgt catataactc tattccagat catgctctaa ttgccataag
     5101 tgaaacatta ggactaactc atacaattca agtattatta ttcataatag ctcatatcgt
     5161 gccagctaca tgggttccat gtgtatctcc tgttttggga gctgggtctc tatcattctc
     5221 tatataatca tatcaatgta tacaatctca taaagcagtt cattctccac taatacatgc
     5281 gctaccatct cgcatattac cactaagatc aggatgatta taagaaattc cattatcaat
     5341 aatagcaatt atggttcatg ttcaatcaga tcatcagcta aatatattca taccagatag
     5401 ccatgatatt gcggataatc atcgtaattc ttcgaaacga ggatcatttg gagtagaata
     5461 agtatgatag atatagttgg gttgtgcata ttctacattt ggatcattat tgagattatc
     5521 caccatttgt tctactgttt cttctcattg gattttcata actacaatat tataattttc
     5581 aatattatct gtaatttcca tatttttatt ttgagcgaaa attttaataa tattttgtca
     5641 tcttttggtg gaaatatcag cttgagaagt tttgtatttt acaaggactt ctcctgggac
     5701 atatttgggt gatacggtat gttgtgtgtt ttgtgctatt
//
LOCUS       DAOSID010000043         5613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_12964_length_5613_cov_15.077726, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000043 DAOSID010000000
VERSION     DAOSID010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12964_length_5613_cov_15.077726"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="PK674_02455"
     CDS             <1..893
                     /locus_tag="PK674_02455"
                     /inference="COORDINATES: protein motif:HMM:NF023746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=25
                     /product="cell division protein FtsZ"
                     /protein_id="HOG15425.1"
                     /translation="QDTDMVFITLGMGGGTGTGAGPVIANIAKEMGILTIGVVTKPFS
                     FEGKKREIQAEEGLAKIKNAVDTLIVIPNDKIFNVIDKKTTFKQAFLMIDKILYSGIK
                     GISDLITKPGDINIDFADIRVVMQNSGNALLGIGYGAGEKRAVDAARKAIENPLLETN
                     IDKAKNVIFAVAGGLDLTPTEVQEAASVIEDIIDPEVNMIWGMSLDESMEDEVQVTII
                     ATGFEEQSKDPIIKSPNRDMLGRPTAPKKVTESFIERIVRTNNEDDNDEIDTEEDNNQ
                     WEGEDLETPAFMRKRLNPEN"
     gene            993..1433
                     /locus_tag="PK674_02460"
     CDS             993..1433
                     /locus_tag="PK674_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOG15426.1"
                     /translation="MQLKISSPAKTIFEGEIKEVILPTEIGEVKILPGHTPMVTVLKP
                     GIINLIPVGSISSNDFIVGKNNLVSLSIGKGMAFVDGKVVKVVISSATTIPTESIKEL
                     ESKKLKIEEKIKSLRKSGSLEEIERSLIQLDKINADIRLKNLKN"
     assembly_gap    1617..1716
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1717..2706)
                     /locus_tag="PK674_02465"
     CDS             complement(<1717..2706)
                     /locus_tag="PK674_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="VanW family protein"
                     /protein_id="HOG15427.1"
                     /translation="MKKVLKTIVYFFSILAFGAVFTNANNFSVDFSLFVDDIVLVANS
                     NKKISFIKFLSDFNKTKDNKCFISYSKSFVELSPICFLDYNTFKVDVNKSLDYLLEGL
                     NGFSQYYGEHLDHFVYKPADVLYSLSDIDRYRQYKSTMHEKIVVTYMENKGQTIRIDK
                     ESFAKLLFDRFSFYVVPTYLGNRGNCSITNYRLAISKLDGMELKAGEELNLNSLISYD
                     PCACKGTLGNNYMFFGGSCGASTQLFRLSLIMPNLDVIERYSHSKWRAYYYGDKIMGD
                     DAAMFENSMKFILKNNFSSSIYFRVYEQGDWSYLVGVLPYKENNYSEINKSSKG"
     gene            2855..>3376
                     /locus_tag="PK674_02470"
     CDS             2855..>3376
                     /locus_tag="PK674_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15428.1"
                     /translation="MFFAIIGKNKQISLAELELIRPTKLKEITNILFTFDTDFPKLLN
                     KLGGIIKRGEVIEHEQLAQELEGKKIIGTKDKDFGIKLKRQFNIKRFKLVDRIKTDLD
                     VKNKGIEVIKFQDKIGVVRGYQNIKLYEKIDFDKPSRSMKMGMMPAKLTHIMLNIGLQ
                     NYLFNGSNQLYKLD"
     assembly_gap    3377..3476
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3477..3992
                     /locus_tag="PK674_02475"
     CDS             <3477..3992
                     /locus_tag="PK674_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15429.1"
                     /translation="IFDPFAGSGTSGFLANYLGYDFIGSDIDITHLEKNKIRRDKQSE
                     KNNKSFEIFQHDISESLPKEKINGNILIVSEGRLGPMVTDKTPKNDIIKYQKKVLELY
                     KQFISTISEIKEKNNVKAIFTIPYYINQNNFLEEEISDFSQKKGRNFGSIKDIYSREK
                     QKVARKIIILK"
     gene            4016..4252
                     /locus_tag="PK674_02480"
     CDS             4016..4252
                     /locus_tag="PK674_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15430.1"
                     /translation="MIEKNTSKAEIGKSIRMNILIASHTHQLNNILKELELRKITKEE
                     FLEKQNAEFKRHQNLVEALKQDIEAENKYNNSST"
     gene            complement(4242..4886)
                     /locus_tag="PK674_02485"
     CDS             complement(4242..4886)
                     /locus_tag="PK674_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOG15431.1"
                     /translation="MQEPVWGYNLLLFLNEYMKYHNFLLISIPITADVFVFFYPVLLV
                     ILYLVGLFKNKDYYKESALWIFWSGVLSMLFNIVVQFFCDKVRPNILLGLEYEKIETI
                     LHKFLPQSSFPSDHAAMSMGIAVGIILWGTRNKDKKFIYIGIVFVLFSLITGVSRVMV
                     GVHRPTDIIAGSIIGILIPVILFQKNIYKFFKKILIIPLINLQKTIWKLFGVKY"
     gene            complement(4904..5527)
                     /gene="nth"
                     /locus_tag="PK674_02490"
     CDS             complement(4904..5527)
                     /gene="nth"
                     /locus_tag="PK674_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="endonuclease III"
                     /protein_id="HOG15432.1"
                     /translation="MAKKNIDFIFQTLDKLYPNADTELKYQNGFQLLLAVIMSAQATD
                     KQVNKVTDKLFKIVFEPRDVLEMGLDKFTKAISSINYYKTKAKNIYATCEILDKNPEI
                     LSSDLKSLTTLPGVGIKTAKVVSHVLFGTKVIAVDTHVHRVSNRLGLVNTKVPEKTSE
                     SLEKIVPDKYKSIAHHGLILFGRYHCTARNPKCQNCPFKDFCKFFNS"
BASE COUNT         2299 a          744 c          693 g         1677 t
ORIGIN      
        1 tacaagatac agatatggtt ttcataacat tatgaatgtg atgatgaaca tgaacatgag
       61 catgacctgt aatagcaaat atagcaaaag aaatgggtat tttgactata tgagtagtta
      121 caaaaccatt tagttttgaa ggtaaaaaaa gagaaataca agcagaggaa tgattagcaa
      181 agataaaaaa tgctgtagat accctaattg ttataccaaa tgacaaaatt ttcaatgtaa
      241 ttgataaaaa aacaaccttt aaacaagcat ttttgatgat agataaaata ctatattcag
      301 gtataaaatg aatctctgat ttaattacaa aaccatgaga tatcaatata gactttgcag
      361 atataagagt tgtaatgcaa aattcttgaa atgctttatt gggtattggt tatggtgcat
      421 gagaaaagag agcagtagat gcagctagaa aggcgataga aaatccatta ctagaaacaa
      481 atatagacaa agccaaaaat gttatatttg cagttgcatg agggcttgac ctaacaccaa
      541 cagaagtaca ggaagcagct agtgttattg aagacataat agatcctgaa gtaaatatga
      601 tttgggggat gagtttagat gaatctatgg aagatgaggt tcaagtaaca ataatagcta
      661 caggttttga agaacaaagc aaagatccta taataaaatc tccaaataga gatatgcttt
      721 gaagacctac agcaccaaaa aaggttacag aaagctttat tgaaagaatt gtaagaacaa
      781 ataatgaaga tgataatgat gaaatagata ctgaagaaga caataatcaa tgggaagggg
      841 aagatctaga aacacctgca tttatgagaa aaagattaaa tccagaaaat taagaaatgt
      901 caatcaataa atcattacaa agatagtgtt ttttgacttt ttagaataaa gactgtaata
      961 taatattgta ttatatattt aacaaaaaaa caatgcaatt aaaaatctct agtccagcaa
     1021 aaactatatt tgaatgagaa ataaaggagg taattttacc aactgaaatt ggtgaagtta
     1081 aaatattgcc atgacacaca ccaatggtaa ctgttttaaa accctgaata ataaatttaa
     1141 ttccagttgg aagcatatct agcaacgatt ttattgtttg aaaaaacaat ttagtatcat
     1201 tgagtatttg aaaaggcatg gcttttgtgg atggcaaagt agtaaaagtt gttatttcta
     1261 gtgctacaac aatcccaact gaatcaataa aagaattaga atctaaaaaa cttaaaatag
     1321 aagaaaaaat aaagagctta agaaaatctg gaagtttgga agaaatagaa agatcgttaa
     1381 tccaattgga taaaataaat gcagatataa gattgaaaaa tcttaaaaat taaaaacagt
     1441 tttagtaaaa aaaataagca aaaaacttcg tgaaacaatc tacgaagttt ttttatttat
     1501 tctcttcatc acacaacccc tagatatgca agtaaaaatt aacaaagaac cattaaaaat
     1561 ttcaaatata atctttcttg caagtgctag ttctcaccct cctgttgcag gcgcgannnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacct ttggaagatt tatttatttc
     1741 actataatta ttttctttat atggcaacac tcaaactaaa taactccaat ctcattgctc
     1801 ataaactcta aaatatatag aactactaaa attatttttt aaaataaact tcatcgaatt
     1861 ttcaaacata gctgcatcat ctcacataat cttatctcag taataataag ctcgccattt
     1921 actatgagaa tatctttcaa taacatctaa atttggcatt atcaaagata atctaaaaag
     1981 ctgtgtgcta gctccacaag atcatcaaaa aaacatataa ttatttcata aagttcattt
     2041 acaagcacaa ggatcatatg atataagtga atttaaattt aattcttctc atgctttcag
     2101 ctccattcaa tctaattttg atatagctaa tctataattt gtaatcgaac aatttcatct
     2161 attccccaaa taagtaggca caacataaaa cgaaaaccta tcaaaaagta atttagcaaa
     2221 actctctttg tctattctaa tggtttgtca cttattttcc atgtaggtaa caacaatttt
     2281 ttcatgcata gtagatttat attgtctata tctgtctata tctgacaaac tataaagaac
     2341 atcagctggt ttataaacga aatgatctaa atgttctcca taatattgtg aaaatcaatt
     2401 taatcattcc aacaaataat ctaaagattt attaacatca actttaaaag tattataatc
     2461 taaaaaacaa ataggagaaa gctcaacaaa agatttagag taactaataa aacatttatt
     2521 atcttttgtt ttattaaaat cacttaaaaa cttaataaat gatatttttt tattagaatt
     2581 agcaactaaa acaatatcat caacaaaaag gctaaaatca acagaaaaat tatttgcatt
     2641 tgtaaataca gctccaaaag caagtataga aaagaaataa acaatagttt ttaaaacttt
     2701 tttcataatt tatatccaaa ataaactatt tcctttatat tataattaaa acaaagtttt
     2761 ttgtcaaaaa atataccatg aaatattgct tttatatact gttttcatat actgtaaata
     2821 cattaaaaac aacatttatt atcaaaaaca aatcatgttc tttgctataa tatgaaaaaa
     2881 taaacaaata agtttagctg aactagagct tattagacca actaaactaa aagaaataac
     2941 aaatattctt tttacctttg atacagattt tccaaaacta ttaaataaat tatgagggat
     3001 tattaaacga tgagaagtta ttgaacacga gcaattagca caagaattag agtgaaaaaa
     3061 aataatttga acaaaagata aagacttttg aattaaatta aaaagacaat ttaacatcaa
     3121 aagattcaag ctagtagacc ggataaaaac cgaccttgat gtaaaaaaca aatgaataga
     3181 agtaataaag tttcaagata aaatctgagt agtaagatga tatcaaaata taaaactata
     3241 tgaaaaaatt gattttgaca aaccttctcg tagtatgaaa atgtgaatga tgccagcaaa
     3301 acttacccac attatgctca acatctgact acaaaactac ttattcaacg gatccaatca
     3361 actttataaa cttgatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatct
     3481 ttgacccctt cgcatgatct ggaacaagcg gatttctagc aaattattta tgatacgatt
     3541 ttatttgatc agatattgat attacacatt tggaaaaaaa caaaatacga cgagacaagc
     3601 aatcagagaa aaacaataaa tcctttgaaa tatttcaaca cgatatttct gaaagtttgc
     3661 caaaagaaaa aataaattga aatattttga tagtatcaga atgacgacta tgaccaatgg
     3721 ttacagacaa aacaccaaaa aacgatataa taaaatatca aaaaaaagtt ttagaattat
     3781 ataaacaatt tatatcaact atctcagaaa taaaagaaaa aaataatgta aaagctattt
     3841 ttacaatacc atattacatc aatcaaaata attttttgga agaagaaatc agcgatttca
     3901 gtcaaaaaaa atgacgaaat ttctgatcaa taaaagacat ttatagtaga gaaaagcaaa
     3961 aagtagctag gaaaataatt attttaaaat aatttttatt ccttaaaaat tgctcatgat
     4021 agagaaaaat acatctaaag cagaaatttg aaaaagtatt agaatgaata ttctaattgc
     4081 ttcgcataca catcaactaa ataacatttt aaaagaattg gagcttagaa aaatcacaaa
     4141 agaggaattt ttagaaaaac aaaatgcaga gtttaaaaga catcaaaatt tagttgaagc
     4201 attaaaacaa gacatagaag ccgagaataa atataacaat tctagtactt aactccaaac
     4261 aatttccaaa tagttttctg taaattaatt aacggaatta ttaaaatttt cttgaaaaat
     4321 ttataaatat ttttttgaaa tagaatcaca ggtatcaata ttcaaattat agatcatgca
     4381 ataatatctg ttggtcgatg aacacctacc attactctag aaactcacgt aatcaatgag
     4441 aacaaaacaa acactattca gatataaatg aattttttat ctttatttct agttcaccac
     4501 aatataattc atactgcaat tcccatagac atagcagcat gatcagaagg aaaagaactt
     4561 tgaggcaaaa acttatgtaa aatcgtttct attttttcgt attctaatca taataaaata
     4621 tttggtctaa ctttatcaca aaaaaattgt acaacaatat tgaacaacat cgataaaact
     4681 caagaccaaa atatccataa agcagattct ttgtaataat ctttattttt gaaaagacca
     4741 acaagatata atataactaa caaaacagga taaaaaaaca caaaaacatc cgcagtaata
     4801 ggaatagata tcaataaaaa attatgatac ttcatatatt cgttcaaaaa caataacaaa
     4861 ttatatcacc aaacaggctc ttgcatagta aacatattac aaattatgaa ttaaaaaatt
     4921 tacaaaaatc tttgaaaggg cagttttgac atttaggatt tctagctgtg caatgatagc
     4981 gtcaaaataa tatcaatcca tgatgtgcta tagacttgta tttatctggt actatttttt
     5041 caagtgattc tgaagttttt tctggtactt ttgtatttac caatcacaac ctatttgaca
     5101 ccctatgtac atgtgtatct actgcaatta cttttgttca aaataacaca tgggaaacta
     5161 cctttgcagt cttgattcca actcaaggta gagtagtcaa ggactttaaa tctgaagata
     5221 atatttcagg atttttatct aaaatctcac aagtagcata aatatttttt gcttttgttt
     5281 tgtaatagtt aatagaggat atagcttttg taaacttatc taaacccatt tccaaaacat
     5341 ctcttggctc aaaaacaatt ttgaataatt tgtctgtaac tttatttacc tgtttatctg
     5401 tagcttgtgc agacataatt accgccaaaa gtaattgaaa accattttga tatttcagtt
     5461 ctgtatcagc atttggatat aatttatcca aggtctgaaa tataaaatct atattttttt
     5521 tagccataaa aaaacatcta aactaaaatt caacgagctt atttttatga aaaaggtttt
     5581 tatatacaat agaagttgaa aacaaagaca aaa
//
LOCUS       DAOSID010000044         5588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_13032_length_5588_cov_15.583770, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000044 DAOSID010000000
VERSION     DAOSID010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13032_length_5588_cov_15.583770"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1148
                     /locus_tag="PK674_02495"
     CDS             111..1148
                     /locus_tag="PK674_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15433.1"
                     /translation="MSIKKPLIGLVIFGLLFTGFSLSETTPTGGEGETISQSDTEMQN
                     SVGSKLGGVYVRFCNNGLEDLTTKVSGTIGQMEKGKICIVAQNDTSNDVTINFSFFST
                     SVTDAGHTACNQTMENVFGSFIDTPQIQLDVPAGEYIVHNFNIQFPLGIEGKQEGCLF
                     YTNADAGNTDRSINIIVGKAMFLEYFVGDPGEVVNDLQLKILKKEIVNNELIIEVGAE
                     NQGNIDLKTELQGSLTNIFKIKKDFSTTGEVVKVNETFIFPLNFGQLPNYKGLFNIDI
                     TAKHAPHFNLDISNFKIDESVLAEKETSISSFYFAIPWVSLIIILVIIILIILATRKG
                     KQKVLYVEKEK"
     gene            1183..2139
                     /locus_tag="PK674_02500"
     CDS             1183..2139
                     /locus_tag="PK674_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15434.1"
                     /translation="MKHKIKIAKNIFFTISIATGFLYFSVGQNIGDIHTRFCQEIPNN
                     EMNIVTQADKETEICMIFENKSESDVKLNINFVDGAITPNGNRACLDPDKAKPNFGQY
                     ILPGDFSLEVPAKSEKQQSYKIKFPVGYSGISHGCVLYSIQDENKSLGAMKVVFSKSH
                     SIDILVGGVKVKSKIKAKRLSLSGDQIHNRIVLDLENQGNTNQSVNITGTISNFFGYS
                     EYFEISDFLLEAGKRESLSTQNLNLPDYKGFFSIKIDINYQPHYNFHITNIDQTTEYT
                     GPGTISFSKTLILRNWFYVAGGGIIVILLIILTKKIFSSKDK"
     gene            complement(2345..3124)
                     /locus_tag="PK674_02505"
     CDS             complement(2345..3124)
                     /locus_tag="PK674_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="M48 family metallopeptidase"
                     /protein_id="HOG15435.1"
                     /translation="MALYETNSIFLLLGPIIVIWGVVSFFFHRQIIFKFAGAKPITRK
                     ENPEIYNIVENLCISKGLATPKIGIIEDDSLNAFATGWNVKNGWIVFSRGILNKLNKS
                     EIEAVAGHELTHILNKDSLMMIIIVVFVGIIGTLGQVLIRYSGRSRNEKGGNILPLIG
                     LGLLLLGYLIYPLIRLALSRKREYLADAGSVELTKDKYAMISALQKISENPVIESIQK
                     DTVAAMCIENPFSKAKSTSFFSKLLSTHPSIEDRIKMLSSY"
     gene            complement(3662..4273)
                     /locus_tag="PK674_02510"
     CDS             complement(3662..4273)
                     /locus_tag="PK674_02510"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="LemA family protein"
                     /protein_id="HOG15436.1"
                     /translation="MTGEEITTNVVEAGEVVRAGMSVSTMVLILVGFLILYFILRYNS
                     LIRLRNNIKNSLADIDVQMKMRFDLVDNLVNTVKGYAGHEKETLENLTKARTNFLNAS
                     SMDQKLSADQALSGALKSLFAVSENYPDLKANQNFLQLQSELSDIENKIAASRRFFNS
                     TVNEYNSSIETFPTNIVAKLFGFKREEFFATAEVEKAVPKVQF"
     assembly_gap    4310..4409
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4410..4738)
                     /locus_tag="PK674_02515"
     CDS             complement(<4410..4738)
                     /locus_tag="PK674_02515"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="cytidine deaminase"
                     /protein_id="HOG15437.1"
                     /translation="MPINNPLAEKEIKELIKVAKKTRKNAFAHRSMHKIGASVLTMDG
                     KIFGGCNVESAISGLGTCAERCAADNAVSHGHYELRAVCTVDTQLVPICGACLQYILL
                     FSQVSDQ"
     gene            complement(4766..4966)
                     /locus_tag="PK674_02520"
     CDS             complement(4766..4966)
                     /locus_tag="PK674_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005829 - cytosol [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HOG15438.1"
                     /translation="METKDCNGNILQTGDTVQPIKDLQVKGSSQVFKKGEAIKKIRLT
                     DDPDFVEVKGVYLRTEFLKKRK"
     gene            complement(5077..>5588)
                     /locus_tag="PK674_02525"
     CDS             complement(5077..>5588)
                     /locus_tag="PK674_02525"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=25
                     /product="IMP dehydrogenase"
                     /protein_id="HOG15439.1"
                     /translation="VGRPQFTAVYKCAKEAKKHSGFVRADGGIKSPRDMNLALAAGAN
                     HVMFGSLFAGTLESVGDVKYDNDGNMYKDNYGMASKKAVNLRNQNESKFGLLKKEMFR
                     EGISNSKIYIKKGRESVGDIVDDFVAGLRSAMTYVGASNLKEFNEKAIIGVQTNAGFA
                     EGEPVGEMR"
BASE COUNT         1877 a          956 c          785 g         1870 t
ORIGIN      
        1 gttgtttttt tgtgttattt aattgatatt ttgtatcaaa agaatatcaa ctattttagt
       61 tgtattgatc ttatcaaaaa ttttttttat ttaattttaa aaaaaaagaa atgtctataa
      121 aaaaaccttt gattggtctt gtaatttttg gcttattatt cacaggattc tctttatctg
      181 aaactactcc tacatgatga gaaggggaaa ccatttctca atctgataca gaaatgcaaa
      241 actcagtttg atcaaaatta gggtgagtat atgtgaggtt ttgcaataat tgattagaag
      301 atttgacaac caaagtaagc tgaactatat gacaaatgga aaaatgaaaa atttgtatag
      361 tagcccaaaa cgatacttcc aacgatgtaa caataaattt ttcatttttt agtacaagcg
      421 taacagatgc ttgacacaca gcatgtaatc aaactatgga aaatgttttt ggctctttta
      481 tagacactcc acaaatacag cttgatgtgc cagcttgaga gtatattgta cacaacttca
      541 acatacaatt tcctttgtga atagaatgaa aacaggaatg atgcctattt tacaccaatg
      601 cagatgcagg gaacacagat aggtctataa atattattgt gtgaaaggct atgtttttgg
      661 agtattttgt atgagatccc ggagaagtag taaacgattt acaattaaaa atactcaaaa
      721 aagagatagt aaataatgaa ttaataatag aagtttgagc tgaaaaccaa ggaaacattg
      781 atctaaaaac agaattacag ggatctttga ccaatatatt caaaatcaaa aaagattttt
      841 ctacgacagg agaagtggtc aaagtaaacg aaacttttat attccctcta aattttggtc
      901 aattaccaaa ttacaaatga ttgtttaata ttgatattac agcaaaacat gcaccgcatt
      961 ttaatttgga tataagtaac tttaagatag acgaatctgt gttggcagaa aaagaaactt
     1021 ctatttcttc tttttatttt gcaattccat gggttagttt gattataata ttagtgatta
     1081 ttatattgat aatattagca actagaaaag gtaaacaaaa agtactttat gtagaaaaag
     1141 agaaataaga aaacaactta tcatatacaa aaccaatatt gaatgaaaca caaaataaaa
     1201 attgctaaaa acattttttt tacaatcagc atcgctactg gttttctgta tttttctgtt
     1261 ggacaaaata ttggagatat tcataccaga ttttgccaag aaatcccaaa taacgagatg
     1321 aatatcgtca ctcaagcaga taaagaaacc gaaatttgta tgatttttga aaacaaatca
     1381 gagtctgatg ttaaattgaa tataaatttt gtggatggag ctattactcc aaacggaaat
     1441 agagcttgct tggacccaga taaagctaaa ccaaatttcg gtcaatatat cctcccatga
     1501 gattttagcc tagaagtacc agccaaaagc gaaaaacaac aaagctataa aatcaagttt
     1561 cctgtatgat attcaggcat aagtcatggt tgtgtcttgt attctattca agacgaaaat
     1621 aaatcgttgg gagctatgaa agtagttttt agtaagtcgc atagtattga tattttggta
     1681 tgatgagtca aagtaaaatc caaaatcaaa gctaaaaggc ttagcttatc ctgagatcaa
     1741 atccataacc gaatagtttt agatctagaa aaccaatgaa acacaaacca atccgtcaat
     1801 attacgggaa ctatttcaaa tttttttggc tattcagaat attttgaaat aagtgatttt
     1861 ttattagaag ctggcaaaag agagtctttg tctactcaaa acttgaattt accagactac
     1921 aaaggatttt tttctataaa aatagatata aattatcaac cacattataa ttttcatatc
     1981 acaaatatcg accaaacaac cgaatatacc ggccccggga caatatcttt ttccaaaact
     2041 ttaattttac ggaactggtt ttatgtagct ggaggtggga taatagtaat tttgctaatt
     2101 attcttacta aaaaaatttt cagtagtaaa gacaaataaa gcatcttctt aaaacatgct
     2161 tattgacaag catttaccac ctcatcattc accccgctaa gcgggacaag catttaacac
     2221 tttaacattt tatcattcac cccgctaagc ggggttttat cattttatca ttttaacatt
     2281 cactccgcta agcggggcaa gtttcatcat cttatcacga acgaagttcg tattcatcct
     2341 tattttaata actactcagc attttgatcc tatcttctat actaggatga gtagataata
     2401 gtttgctaaa aaaactagta gactttgctt ttgaaaatgg gttttctata cacatagccg
     2461 ctacggtatc tttttggata gactctatca cagggttttc gcttattttt tgtaaagcac
     2521 taatcatagc atacttatct tttgttaact caacactacc agcatcagcc aaatattctc
     2581 ttttacgaga aagtgccagc ctaatcaaag ggtagattaa atatcacaat aacaacaatc
     2641 acaatcatat caaaggcagt atatttcctc atttttcatt tctagatctt ccagaatatc
     2701 taatcaaaac ctgtcccaat gtcccaataa ttcacacaaa aacaactata attatcatca
     2761 tcaaagaatc tttgttcaaa atgtgcgtta gttcatgtca tgcaactgct tctatttcag
     2821 atttgtttaa tttatttaaa attcatctag aaaaaactat ccatcaattt ttgacattcc
     2881 atcaagtcgc aaaagcattt aaactatcat cttctattat tcatattttt ggtgtggcta
     2941 gtcatttgga gatacataaa ttttctacaa tattgtaaat ttctggattt tctttccttg
     3001 taataggttt tgctcaagca aatttaaata tgatttgtct atgaaaaaag aaagaaacaa
     3061 ctcaccaaat tactatgatt ggtcatagta ataaaaaaat actgtttgtt tcatacaaag
     3121 ccatttttcg tgattgaaga aaatatattc attcagagct atccataaat gaaaaagtta
     3181 aaattatgtt tgtaatcaaa aacaataata cagggaatag tattaataaa caaagactcc
     3241 taaatctgtt ttgatgaata gaactataaa ttcattttgg catcccagtt gtttattaag
     3301 ttaaaaagtt gcatccccta ccacccgtcc cgtagtccta cggagcgagt ggtaggaaag
     3361 aaaaaaattc taatattgta tagtcctgca attctgtgga caaacagatt agtaaagttt
     3421 ggagataaaa ttcctcccct tggggaggtt gttctccccc gccccgcccc gcagtattgc
     3481 ggggctacgg gcgggtaggt gggggtttta ccccaacaac ccccaactac gatagagcaa
     3541 cttccctctc ccgatacatc gggactccgc ttcgcttcgc aagggaagaa cttttttgtc
     3601 gtaaagttta taaagattaa taatttgtaa ctttacaaac ttgccgtaac tttctactca
     3661 cttagaactg aacttttgga actgcttttt ctacttctgc tgtagcgaaa aactcttcac
     3721 gcttaaaccc aaacaattta gcaacaatgt ttgtggggaa agtttctata gacgaattgt
     3781 attcatttac agtagaattg aagaatcttc tacttgcggc gattttgttc tctatatcag
     3841 atagttctga ttgtaattgc aaaaagttct gattagcttt taggtctgga taattttctg
     3901 atacagcaaa caaacttttc aaagctccac ttaacgcttg atcagcagaa agtttttggt
     3961 ccatagaact ggcattcaaa aaatttgtcc tagctttagt tagattttca agagtttctt
     4021 tttcatgtcc agcataacct tttacagtat ttacaagatt atcaaccaaa tcaaatctca
     4081 ttttcatttg tacatcaata tcagctagag aatttttgat gttgtttcta agtcttatca
     4141 acgaattata ccttaaaata aaatacaaaa tcaaaaatcc aactaaaata agtaccattg
     4201 tgctaacact cattcctgcg cgaacaactt ctcacgcttc taccacattt gttgtaattt
     4261 cttctcaagt catttttttg tagatttaaa taataaaaga ttattttttn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cttgatcaga aacttgagaa aacaataaaa
     4441 tatattgcaa acaagctcag caaatgggta caagttgggt atctacagtg catacagccc
     4501 taagctcgta atgtccatga gaaacagcat tgtctgcagc acagcgttct gcacaagtac
     4561 caagcccact aatagcgctt tctacattgc atcatcaaaa aatttttcaa tccatagtca
     4621 aaacactagc tcatattttg tgcatggaac gatgagcaaa agcattttta cgagtttttt
     4681 tggctacttt gatcaattct ttgatttctt tttctgcgag aggattgttt attggcatag
     4741 aaactaattt tatattttaa aacaactatt ttcttttttt caaaaactct gttctcaaat
     4801 aaactcattt cacttccaca aaatcgggat catctgtaag acgaattttc ttgatagctt
     4861 ctcatttttt gaatacttgt gaacttcatt ttacctgaag atctttgata ggttgaactg
     4921 tatctcccgt ttgtaaaatg tttccattac aatcttttgt ttccataatt ttttcttagt
     4981 aaataaattc ttacttttag cataagattt cctttctcct tttcaaatga ttttgataaa
     5041 acgagaactt attctataaa ccttatctta tagatcttat ctcatttctc ccacaggttc
     5101 tcattcagca aaacctgcat tagtctgtac tcaaataata gctttttcat taaattcttt
     5161 taaattacta gctcatacat aagtcatagc agaacgaagt catgcaacaa aatcatctac
     5221 aatatctcat actgattctc ttcacttttt tatataaatt tttgagttag aaattcattc
     5281 acgaaacatc tcttttttca acaatcaaaa ctttgattcg ttctgatttc tcaaatttac
     5341 tgctttttta cttgccattc cataattgtc cttgtacata ttcccatcat tatcatattt
     5401 tacatctcat actgattcta aagtcccagc aaataaggag ccaaacatca catgatttgc
     5461 tcaagcagct aaagctaagt tcatatctct tggtgacttt attccaccat ccgcccgtac
     5521 aaaaccacta tgtttttttg cttcttttgc acatttgtat actgctgtaa attgaggtct
     5581 tcatactc
//
LOCUS       DAOSID010000045         5499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_13293_length_5499_cov_13.654849, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000045 DAOSID010000000
VERSION     DAOSID010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13293_length_5499_cov_13.654849"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PK674_02530"
     CDS             <1..817
                     /locus_tag="PK674_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15440.1"
                     /translation="ASSIFIIFLYLKFSRTVLDLSSFFEINRGEKTGCDSFFYLQDSS
                     VVDPEYVIKTLKTTRGLTRYTIIPNTNTFICSDSIDNLTDSGKTLTIKWIDRHQGCKD
                     SINKKFIEWYDFPRNIEVDFVINVNPNKVIAVYELNKRCYVGQRGIGLQIHEGLNKNL
                     LFALMNSIISLFFIEAAGFGRGLGALDLNSDKIKNLKILDPSLLNAEQMTGIIEKFNA
                     LKQREIKELDEELVSSDRVEFDKYVLECYGVLDYYENVKSALLQMYNTRISVE"
     gene            1240..2046
                     /locus_tag="PK674_02535"
     CDS             1240..2046
                     /locus_tag="PK674_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15441.1"
                     /translation="MKIYFKGDVYDKKFENFRGDVEYLLAHFSYPTLEEIEKEDKFIF
                     MCYNMIVYIIGGKDIIIKDEIKVNPKKYYPKNWKIGDNIPSDTVNKMVAAKLGIDWEL
                     FRNILHSTNGSEKKDGSEKKESYKKKVFYQLNQIMTNKSDNYLNEIIKSLQPGELLIL
                     TRTDDGRIFKGKGNGGIPNGIGRWTNNDGDVFWGKFIDGIASGPGFVKLNDGQRFEGI
                     RENGAMKAKGLEKGGITFHIEALNYDNIVDKMIIQRNEDGGLSVFKNRDY"
     gene            2263..2568
                     /locus_tag="PK674_02540"
     CDS             2263..2568
                     /locus_tag="PK674_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15442.1"
                     /translation="MNEKNLSKEFEEMDAFEFHSFLRKLNRSTQNKLLVIIRTGDISK
                     AKRLKAIKENPAFYDKLEFQLEIEATVQEYETELNVFASGINGLKKNNILTLKIFST"
     gene            2767..4764
                     /locus_tag="PK674_02545"
     CDS             2767..4764
                     /locus_tag="PK674_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="AAA family ATPase"
                     /protein_id="HOG15443.1"
                     /translation="MKISRIKISNLFTFPYISGFHGKKGVIFDARERNNFNIIIGPNG
                     SGKSNFLHIISQTFRFGLVNHYFYDKSILENNEKNKFKQVIQVKKKKIKNMPKHYLSG
                     DKPSKIGITLKLNQNDFENIAFMSKHTILLNNILKKYSKLDISFSDYHSYDLIFEHEI
                     YLEFLVNSKNGSFKIANYKFLSNKMRFILFYIQNIELIQICINIFNDFEKLPEQKKLY
                     SLKNTFALLESERNILYDNTGEYQPFLVDSVAARSEDIELHKEHKFASVTVGYDLFII
                     KLIKLAKEKLLFEGKNYTLESPKVQLKALTSSYYFRETAYFIKKILKLDLDIKISDKG
                     LEIVFVNKYGNLIYFDQLSSGEKSILMILIALFGYDLSSGLFIIDEIELHLHPYLLKK
                     MVDLLKEIGAKLNIQFICSTHSPILIDEQSINNVYKFSFVDGQTNIHYPMNSIRDKEA
                     TLIHILKFEHIAKIFFMNKILMVEGETDEYFWRFFLSYLSKKPEFKGKLDNFEILNIN
                     GKGSYSRWSNFLKKFGIESYFIGDWDNVVGNGIVDYQEMDTYINLTKQCLDSDHIKKE
                     KFYVRIIETIKKFFPEKYEYIKNKIQLFYDQGIFILQEGNLEVYLGLKRKGLEETIRF
                     CNNNFYSWLNNKNYKKHFQEFKEIVAQIFKLKKRDKLVKSL"
BASE COUNT         2161 a          582 c          755 g         2001 t
ORIGIN      
        1 cgcctcctca attttcatta ttttcttata tctaaaattt tcccggacag tactggatct
       61 ttctagtttt tttgaaataa atagatgaga aaaaacatga tgtgattctt tcttttactt
      121 acaagattca tctgttgttg atccagaata tgtcataaaa acattaaaaa caacaagatg
      181 attgacgaga tatacaatta tacctaatac aaatactttt atttgtagtg atagtattga
      241 taatttaact gattcctgaa aaacattaac tattaaatgg attgatagac atcaatgatg
      301 taaagattca ataaacaaaa aatttattga atggtatgat tttccacgta atattgaagt
      361 agattttgtc attaatgtga atccaaataa agttatagcc gtatatgaat taaacaaaag
      421 atgttatgtt tgacagagat gaatatgatt acaaatacat gagtgattaa ataaaaattt
      481 attatttgcc ttaatgaata gtataatttc cctgtttttt attgaagctg cttgattttg
      541 aagaggtcta tgagcacttg atcttaactc agataaaata aagaacttaa aaattttaga
      601 tcccagtttg ttaaatgcag aacaaatgac atgaattata gaaaagttta atgcattaaa
      661 acaaagagaa ataaaagaac ttgatgagga gctcgttagt tctgatagag ttgaatttga
      721 taaatatgta cttgagtgtt attgagtttt agattattat gaaaatgtta aatctgcatt
      781 acttcaaatg tataatacca gaatatctgt agaataagag attttactat ataaagatta
      841 tgtatttaag taaaattttt ataaactata taagtataaa tgtttcaatt gattgaatta
      901 ataaaatttg gaaaaataaa attaatttgg attatatagt ttatataaga aataaattat
      961 taaaaatctc ttgcaatcgt aacaggaata aaatataatt aaatcggatt caagaatgtg
     1021 gatggattta tacctacttg caaaccacta taaaaaaatg ctaaagggcg atcggaatgt
     1081 tgttatattc accgttctta catttcaaat tataggcaaa gactataaat actgaataca
     1141 tttaagaatc tcatacagat ttggccaaat gtattttttt gttttatcga taacatacag
     1201 atctttctgt gcttatttat ttagtaacta aaatttacca tgaaaatcta ttttaaatga
     1261 gacgtatatg ataaaaaatt tgaaaatttt agatgagatg tcgaatattt actagctcat
     1321 ttttcatatc caacactgga agaaattgaa aaggaagata aatttatctt tatgtgttat
     1381 aacatgatag tgtatattat ttgatgaaaa gatataatta taaaggatga aatcaaagtt
     1441 aatcctaaaa aatattatcc taaaaattgg aaaatcggag acaacatacc ttcagatact
     1501 gtcaataaaa tggtagcagc caaattatga atcgattggg aattatttag aaatattctt
     1561 cattctacaa attgatctga gaaaaaagat tgatctgaga aaaaagaatc gtataaaaag
     1621 aaggtatttt atcagcttaa tcagatcatg actaataaaa gtgataatta tttaaatgaa
     1681 ataattaaat cattacagcc ttgagaactc ttaattttga caagaacaga tgattgacga
     1741 atctttaaat gaaagtgaaa cggttgaatc cctaattgaa tatgaagatg gacaaataat
     1801 gactgagatg ttttttggtg aaaatttatt gattgaattg cctcatgacc atgatttgta
     1861 aaattgaatg actgacaaag atttgaatga atacgagaaa attgagctat gaaagctaaa
     1921 tgattggaaa aatgaggaat aactttccat atagaagcct taaattatga caatattgta
     1981 gataaaatga ttattcaaag aaatgaagat tgatgattaa gtgtattcaa gaatagggat
     2041 tactaattat tcttttataa cgtataacga ttatcaaaaa ggctgaataa attaaaaatc
     2101 attttacccc ctgctataaa aaagtttgta gcataaaata aatcataaga atttgaaaaa
     2161 aacaaaaatt ctgactatac taatagggag tctgttttcc ttattttaac tcctccactc
     2221 aaccatttag taatcattat ttataatata tatttatatt aaatgaatga aaagaatctt
     2281 agtaaagagt ttgaggaaat ggatgctttt gaatttcata gcttccttcg taagctaaat
     2341 agatcaactc aaaataaatt attggtgatt attcgtacgg gtgatatttc aaaagcaaaa
     2401 agattaaagg caattaaaga aaatcctgca ttttatgata aattggaatt tcaacttgaa
     2461 attgaagcta cggttcaaga atatgaaaca gaactaaatg tgtttgcttc tgggataaat
     2521 ggattgaaaa aaaataatat tctaacttta aaaatatttt ctacataatt tactttgcac
     2581 aatttttttg aagacttgaa ttttttataa taaatttagt ttttactgga ggattttaac
     2641 acaatattta atataatttt ggtttttgta aacctccact actctgtttg tgtaataaaa
     2701 atttgataat tgtttatatt tgcttataat gaatatatgt taattttatt ttagattata
     2761 ttttttatga aaattagcag aataaaaatt tcaaatctat ttactttccc ttatataagt
     2821 tgatttcatt gaaaaaaagg tgtgattttt gatgcaagag aaagaaataa ctttaatatt
     2881 attatttgac caaatggatc tgggaaaagt aattttttgc atataataag ccaaactttt
     2941 agattttgat tggtaaatca ttacttttat gataaaagta ttttggaaaa caatgaaaag
     3001 aataaattta aacaggttat acaggtaaaa aagaaaaaaa taaaaaatat gccaaaacat
     3061 tatttatctg gtgataaacc ttcaaaaatt ggtattactc taaaactcaa tcaaaatgat
     3121 tttgagaata ttgcttttat gtcaaagcac accatcttac taaacaatat attaaaaaaa
     3181 tattcaaaat tggacataag ttttagtgat tatcattcct atgatcttat ttttgaacat
     3241 gagatttatt tggaattttt agtcaattca aaaaactgat cttttaaaat cgccaactat
     3301 aaatttttgt ctaataagat gagatttatt ttgttttata tacaaaatat agaattgatt
     3361 caaatatgta ttaatatctt taatgatttt gaaaaattac cagaacagaa aaaattatat
     3421 tctttgaaaa atactttcgc attattagaa tcagaaagaa atattctata tgataataca
     3481 tgagagtatc agccatttct tgttgattct gttgcagcta gaagtgagga tattgaatta
     3541 cataaggagc acaaatttgc ttctgttact gtatgatatg atctatttat tataaaactt
     3601 atcaaacttg caaaagaaaa gttgttgttt gaatgaaaaa actatacatt ggaaagtcca
     3661 aaggttcaat taaaggcctt gacatcgtct tattattttc gtgaaacggc ctattttata
     3721 aagaaaatat taaaactaga tctggatatt aaaatctctg acaaatgatt agagatagtt
     3781 tttgtgaata aatatggaaa tttaatttat tttgatcaac ttagttcttg agaaaagtct
     3841 attttgatga ttttgattgc attatttggt tatgatttat caagtggatt gtttataata
     3901 gatgaaatag aattacatct acatccatat ttattgaaaa aaatggtaga tcttttaaaa
     3961 gaaatatgag caaaacttaa tatacaattc atttgttcta ctcactcccc tattttaatt
     4021 gatgagcaaa gtataaataa tgtttataaa tttagttttg ttgattgaca aacaaacata
     4081 cattacccta tgaattctat tcgtgataaa gaagctacac ttattcatat tttaaaattt
     4141 gaacatattg caaaaatatt ttttatgaat aaaatactta tggttgaatg agaaactgac
     4201 gaatacttct ggagattttt tttaagttat ttgtctaaaa aacctgaatt taaatgaaaa
     4261 ttagataatt ttgaaatttt gaatataaat tgaaaatgaa gttattctag atggagtaat
     4321 tttttaaaaa aatttggtat agagtcttat ttcatttgag attgggataa tgttgtatga
     4381 aatggtattg ttgattatca agagatggat acttacatta atttaactaa acaatgtctt
     4441 gattccgatc atataaaaaa ggaaaaattt tatgtaagaa ttattgaaac aataaaaaaa
     4501 tttttccctg aaaaatatga atatattaaa aacaaaattc aactttttta tgatcaatga
     4561 atttttattt tacaggaatg aaatttagaa gtttacttat gattaaaaag aaagggattg
     4621 gaagaaacca ttagattttg taataataat ttttactctt ggttgaataa taaaaattat
     4681 aaaaaacatt ttcaagaatt taaagaaatt gttgctcaaa tttttaaact taagaaaaga
     4741 gacaaattag ttaaaagttt ataaagttgc aaattgaaag ttacacaagt tataaagttg
     4801 aaaggttcgt aggggtgaat attcttggtg ttcttaattt ccgctattca tacggggtaa
     4861 attgggtata cggtaatcgt ctgctgttta gaccgtagta aaccagagta ttatggtaaa
     4921 aaaattactc gctttatata taattaactt tttcaaaaac aaaaaagacc aactgaaaca
     4981 gtcggtccgt ccttacatag gttgtacaac ctagagacgg aacaactctt tatcataata
     5041 tagagtttat taatagactt cgttttttta gtaccaaatc tatttataaa cactatatat
     5101 tttttggcat tacaattagg ctacattaaa accaaaaaaa ccgtctctag gattttttgc
     5161 ataaattttc gttttggtaa actactcttt tatgcattta aatctaattg aatacaattt
     5221 agatttaaat taaaatcttt gcttttttgt taaataattt tatttacgat tccaaaatat
     5281 ctatatataa aaataaaaca acaaaaaaaa caaaaaatca tctaatcttt tgttagcaca
     5341 tttttaatta tttattatat agtatatcta atatagtata tttctgttgg tactactctg
     5401 ttcaatctgt tgtattatct ttgaaatcga tgaacttcat aatacttgct ttgaaataca
     5461 attctatttc tcaaacttct cagtttctgt tttttctta
//
LOCUS       DAOSID010000046         5466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_13389_length_5466_cov_15.810571, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000046 DAOSID010000000
VERSION     DAOSID010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13389_length_5466_cov_15.810571"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..3629
                     /gene="aspS"
                     /locus_tag="PK674_02550"
     CDS             279..3629
                     /gene="aspS"
                     /locus_tag="PK674_02550"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOG15444.1"
                     /translation="MKTKDLSKRKFSSGTKRSALDVKVNQVNGIYSQQNANINNFSNI
                     LNLNQELQELGLELVIGIETHITVTNTKTKLFCSCLNDTESAPNTNICPTCTGKDTSS
                     PQVNLDVVKSATFLSNALGGELANTLSFDRKHYSYPDLPKGYQITQYRDPISKGGSVP
                     FMRPDGTEGKVDIDHFHIEEDPAKLTYLKNGKIDLDFNRSGKPLFEVVTTPSIHSIED
                     AILYMEQLQKIVRYTGVSDADMEKGHFKSDVSLSLRKAGTNDLNPRTEIKNLNSFKFA
                     LQALHLEVEEQLNIRKNTKAPQKQQQTKGWREDKLALEVMREKETEADYNYIPEPNIP
                     ERDITEIVSEKIDFEFLPYQVEKQLMDSGLSYQDAIFFSADLDKIIALYTLSDEKGLY
                     DTAKLLINLPQSVRYDNTVSLSIIKKLFALFSQSKYFDQTLLKIVLDYLSKDPDFDFE
                     QFIDEKTHLTDDLKEAIRKVVAKNPNKSVGSIIGQMIQKNILPQYFSRSLVLEFIGSL
                     GKTNDQIKTLEKVKGSKNTAEKTYEYDFDTFLKPYRTHSISEISNSLENQKVTTSGWI
                     QSIRDHGDLVFIDLRCKGEILQIQIDKKSDINLDEITRWADESVIKVEGTLIKRSPDD
                     INERLRLGIIELKADKIELLSGSQNTPFEVPQSHKVNENLRMTYRYLDLRNPHIMENV
                     IKRHEVTQYIRSFFDQRGFLHVDTPILGKPSDEGSREFSVPSRLHPSSFYTLPQAPQQ
                     LKQLLMGSGVDKYYQIARCFRDEDPKGDRQPEFTQVDMEMAYIQEEDIVALISELVVG
                     ILEKNYPHKKLITKTIPHYDYNYIMEHYSTDKPDLRYGLKMYTLTDMVKDCGFDVFAN
                     VVAKGGIVKAMLVEGGKKMFSKKYLDVTLKEFTQQLGGGGLANFHVGGEANFVEEKLG
                     KELVAKIIEKTGAKEGDTLLFVADQESTAHQILSQTRQKIAKDYKLYKEDDISLLRVR
                     NFPMFEKTDEGKRKFTHNPFSMPKIECLKDFMEGKNIPNIMAQQYDIVMNGNEVGGGS
                     IRAHLPELLQQTYKIMGYTPEETQKSVGHMLEAFKFGFPPHGGLALGLDRILMILQGH
                     DTIREVIPFPKTGDGKDLLMGAPAPRSS"
     gene            3797..5422
                     /locus_tag="PK674_02555"
     CDS             3797..5422
                     /locus_tag="PK674_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15445.1"
                     /translation="MNISIGFSGPAGSGVNTAGLLLGELLSPKGFNVLADKEYVSIIK
                     GGNNCFFLYITDNDTPYISKTIDLFFAFDDYAITKNEKIYDLKNILNIKTFAKKHKNI
                     FAFGVSLKALGIPKEEGIVLLEKTFSQAVLDENKEELLAGYEYFDTVQLDFKIDLDFS
                     KNIGPQKLFMFGNELIAKGAIDSGLEFYSAYPMTPASSIIDIIVKYPEKVTFFQGEDE
                     IAVGMSMLGAKFAGKRAMCGTSGGGFALMSEGISFSNQAEIGGVYVLSQRDGPSTGTP
                     TFTAQGDLFYAMNASFGDTQPIVYAPSKFEELYTDIGKALNWSDIYQHPVIFLLDKQL
                     SESYKSIATDLQAEPIDRGIIAESKQNDEYLRYKLTDTGISPYAVPGQENTTFIATSY
                     EHTESGETNEDTVIKGQHMEKRMKKLQTFVQKEFNESFYGYEIINPNAKKFYVTRGMN
                     RYSIEYAIQNKADWGLIVIKSFQPFDKRLKTFFQEKKDNIEKLVFVEMNYLGQMQQVV
                     MRECNLLDDSWQGKISHIRKNGLYPFFYEEITD"
BASE COUNT         2039 a          781 c          872 g         1774 t
ORIGIN      
        1 ttctgataaa agtgactttt catcaaaaaa acatatacta ataaataatt tgcaattgga
       61 tataaaatac ttataatgat attgtaatca tttaaattca atcacaaaat gatggtaaaa
      121 cttagatcat atcaattaaa taataataat ctacggatac cgtagatgat ttgttattga
      181 ttaaaataat aaaaaatctc cacggtatct aaaaaaaccg ctggagattt ttttaatctt
      241 gtattgttaa ttaatcttta tatattaccc aatatatcat gaaaacaaaa gacttatcta
      301 aacgaaagtt tagtagtgga actaaaagat ctgctttaga tgttaaagta aatcaagtaa
      361 attgaattta ctcacaacaa aatgccaata taaataattt ttccaatata ttaaatttga
      421 atcaagaatt acaagaactc tgactagaac ttgtgatatg aattgagacc cacatcacag
      481 ttaccaatac aaaaactaaa ctattttgtt cttgtctaaa tgataccgaa tcggcaccta
      541 acacaaatat ttgtcctact tgtacaggaa aggatacttc ttcgcctcag gtaaatttag
      601 atgttgtcaa aagtgccacc tttttatcaa atgctttgtg atgagaactt gcaaacacct
      661 tatcatttga tagaaaacat tattcttatc cagatttacc aaaatgatac caaatcactc
      721 aataccgtga tccgatttct aagtgatgat cagttccttt tatgagacct gatggaacag
      781 aatgaaaagt ggatattgat cattttcata tagaagaaga tccagctaaa ttaacttatt
      841 taaaaaattg aaaaattgat ctggatttta atagatctgg aaaaccactt tttgaagtag
      901 taaccacacc ttcaattcat tccattgaag atgctatctt atatatggaa caacttcaaa
      961 aaattgtaag atatacttga gtttcagatg ctgatatgga aaaatgacat ttcaaatcag
     1021 atgtgagttt atcgcttaga aaagctggga caaacgatct taacccaagg acagaaatta
     1081 aaaacctaaa ctctttcaag ttcgctttac aagcattaca cttggaagta gaagagcagt
     1141 taaatattcg aaaaaacacc aaagctccac aaaaacaaca gcaaacaaaa tgatggagag
     1201 aagataaact agctttggag gtaatgagag aaaaagaaac tgaagcagat tataattata
     1261 ttccagaacc gaatattcca gaaagagata ttacagaaat tgtttctgaa aaaatagatt
     1321 ttgaattttt accttatcag gtagaaaaac agctgatgga ttctggattg agctatcaag
     1381 atgcaatctt tttctcagca gatcttgata aaattattgc tctttacaca ttgtctgatg
     1441 agaaaggact ctatgataca gcaaaacttt taatcaatct gcctcaatcc gttcgatacg
     1501 ataatacagt ttctttgtcc ataattaaaa agctttttgc tctcttttct caaagcaaat
     1561 attttgatca aacacttttg aaaattgttc ttgattatct aagcaaggat ccagattttg
     1621 attttgagca gtttattgat gaaaaaactc acttaactga tgatcttaaa gaggctattc
     1681 gtaaagtggt ggctaaaaat cctaataaat cagttggttc tattattgga caaatgatac
     1741 aaaaaaatat tttgccacaa tatttttctc gttcacttgt tttggaattt ataggaagtc
     1801 tttgaaagac taatgatcag attaaaacct tagaaaaagt aaaaggttct aaaaatacag
     1861 cagaaaaaac ttatgaatat gattttgata cttttttgaa gccctataga actcattcta
     1921 tatcagaaat atcaaatagt ttagaaaatc agaaagttac tacttcttga tggattcaat
     1981 ctattcgtga tcatggagat ttggttttta tagatcttcg ctgtaaagga gaaattttgc
     2041 aaattcaaat tgataaaaaa tctgacatca atttagatga aattactcgt tgggcggatg
     2101 aaagcgtgat taaggtagaa tgaacactta tcaaaagatc tccagatgat atcaatgagc
     2161 gtttgaggtt aggaatcatt gaactaaaag cagataaaat agaacttctt tcaggatctc
     2221 aaaacactcc ttttgaagtt ccacaatctc acaaagtaaa tgaaaattta aggatgacct
     2281 ataggtattt ggatctcaga aatcctcata ttatggaaaa tgttatcaaa agacatgaag
     2341 ttactcagta tatcaggagt ttttttgatc aaagatgatt tctacatgta gatactccta
     2401 tcttgggaaa accaagcgat gaatgatcta gagaattttc tgtccctagt agactccatc
     2461 cttccagttt ttatacttta cctcaagctc ctcaacaact taaacaatta cttatgtgat
     2521 ctggagtaga taagtactat caaattgcga gatgttttcg tgatgaagat ccaaaatgag
     2581 atagacagcc agaatttaca caagtagata tggagatggc ctatatccaa gaagaggata
     2641 ttgttgctct cattagtgag ttggtagtat gaattttaga aaaaaactat cctcataaaa
     2701 aacttattac taaaactatt cctcactatg attataatta tattatggag cattatagca
     2761 ctgataaacc agatttgaga tactgactca aaatgtatac acttacggat atggttaagg
     2821 attgtggatt tgatgttttt gcaaatgttg tcgcaaaatg atgaattgta aaagctatgc
     2881 ttgtagaatg atgaaagaaa atgtttagta aaaaatatct tgatgttact ctcaaagaat
     2941 ttactcaaca acttggatga tgaggtttag caaactttca tgtatgatga gaagctaact
     3001 ttgtagaaga aaaacttgga aaagaacttg ttgcaaaaat tattgaaaaa actggagcaa
     3061 aagaatgaga tactttactt tttgtagcag accaagaatc aacagctcat caaatccttt
     3121 cacaaaccag acaaaaaata gctaaagatt acaaattata caaggaagat gacatttcac
     3181 ttttacgagt aagaaacttt ccaatgtttg aaaaaacaga tgaaggaaaa cgaaaattta
     3241 ctcataatcc tttctcaatg cctaaaatag agtgtttaaa agattttatg gaaggaaaaa
     3301 atatccctaa tattatggca caacaatacg atattgtaat gaactgaaat gaagtatgag
     3361 gctgatctat tagagctcac ttaccagagt tgttacaaca aacatataaa attatgggct
     3421 atactccaga agagactcaa aaaagtgttg gacatatgct agaagctttc aaattctgat
     3481 ttccacctca tggatgattg gctttaggat tggatagaat tctgatgatc ctccaatgac
     3541 atgatacgat tcgtgaagtt attcctttcc caaaaacagg agatggaaaa gatttactta
     3601 tgggagctcc tgcaccaaga agtagttaaa ttttaaataa ataactatga tatgtttttt
     3661 taaaaatact gtttttgtaa gaaatatatt tttacatcac attattttgt cattcaataa
     3721 tttttctttt cttgcttttt cgtactaatt tcttaaacct aaaagtgcaa tatttttgtt
     3781 ttttgtaaaa actactatga atatatctat atgattttcg tgaccagctg gatcctgagt
     3841 aaatacagct ggattattgc tttgagagct actttcccca aagggcttta atgttcttgc
     3901 tgataaagaa tatgtttcaa ttattaaagg tggtaataat tgtttttttt tatatatcac
     3961 tgataatgat acaccttata tctccaaaac aatagactta ttttttgctt ttgatgatta
     4021 tgcgattaca aaaaacgaaa aaatctatga tctaaaaaat attctaaata taaaaacttt
     4081 cgcaaaaaaa cacaaaaata tttttgcctt ttgagtttcg ttaaaagctt tgtgaattcc
     4141 aaaagaagag gggattgttc ttttagaaaa aactttttca caagcggttt tagatgaaaa
     4201 taaggaagaa ttgttagctt gatatgagta ttttgatact gttcaacttg attttaaaat
     4261 agatttagat ttttctaaaa atatttgacc tcaaaaattg tttatgtttg gaaatgaatt
     4321 gattgcaaaa tgagcgatag atagttgatt ggaattttat tctgcatatc caatgactcc
     4381 tgcatcaagt attattgata ttatcgttaa atatccagaa aaggtaacat tttttcaatg
     4441 agaagatgaa atcgcagttg gtatgtctat gttgtgagca aagtttgctg gtaagagagc
     4501 tatgtgctga acttctggat gatgatttgc tttgatgtca gagggtattt cattttcaaa
     4561 tcaagcagaa atatgatgag tctatgttct ttcacaaaga gatggtccaa gtacaggaac
     4621 tccaactttt acagcacaag gagatttatt ttatgcaatg aatgcttcat ttggagatac
     4681 tcaaccaata gtttatgcac catcgaaatt tgaggaatta tatacagata tatgaaaagc
     4741 gctaaattgg tctgatattt atcagcatcc tgttatattt ttgttggata aacaactttc
     4801 cgagtcatat aaatctattg ctacagactt gcaagctgag ccgatagatc gtggaataat
     4861 cgcagaatcc aaacaaaacg atgagtactt gagatataag cttactgata ctggtatttc
     4921 tccttatgct gttccaggtc aagaaaatac aacatttatt gcaacatcat atgaacatac
     4981 agaatcatga gaaacaaatg aagatacagt tataaaatga cagcatatgg aaaagagaat
     5041 gaaaaaatta caaacattcg ttcaaaaaga atttaatgaa tcgttttatg gatatgaaat
     5101 aatcaatcca aatgcaaaaa agttttatgt aacacgaggt atgaatcgtt attcaataga
     5161 gtatgcaatt caaaacaaag cggactggtg acttattgtc ataaaatcat tccagccatt
     5221 tgataaaaga ttgaaaacat ttttccaaga aaaaaaagat aatatagaaa aactcgtttt
     5281 tgtagagatg aattatttgg gacaaatgca acaagttgtg atgcgcgaat gtaatttact
     5341 agatgattct tggcagtgaa agatttctca tataagaaag aattgacttt accctttctt
     5401 ttatgaggaa ataacggact agatggttat taaccacctc tttgttctct cctttttaat
     5461 ataaat
//
LOCUS       DAOSID010000047         5443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_13458_length_5443_cov_15.860431, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000047 DAOSID010000000
VERSION     DAOSID010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13458_length_5443_cov_15.860431"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..2544
                     /locus_tag="PK674_02560"
     CDS             112..2544
                     /locus_tag="PK674_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="3'-5' exonuclease"
                     /protein_id="HOG15446.1"
                     /translation="MQKVKEYLAGKLNEEQLKAALHTDTSSLILAGAGSGKTRTLTYK
                     IGYLMFGLKINPNNILAVTFTNKAANEMKERLMEISDDFSQMMGGTMSDDCHQKIGTM
                     SDDFSQKISDDKSSDIDDNRQTLPPDDNRQTLSPEDSIDDFLNFIEQTKPSIGGNNVK
                     YTEKDFKWIGTFHSIFLKILKEDIDKLEMKYDKNFTIIDSNDSNKVVKDLLKKLNLDE
                     VFKPNEVKGFISNQKNNGYDAKMYQSKITTDYEQKMGKVYVEYQKELERSNMLDFDDL
                     LLLPYLLFRKRADILEKWKKKFLYIMVDEAQDTNWIQFELMMMLSGKDGNITLIGDDY
                     QSIYGRRGALMENFLNVKKYWPDIQMFKLQINYRSRPHIVNAGNYIIKNNLKQYNKDV
                     HSHRKEDGKITVFCHNSDIDEAANIIDFIMKMKDKGRIQKLGDVSILYRTNAQSSTFE
                     QILVQEKIPYKIRGAYKFFEREEVKDILAYIKYLANSGDSVSLKRIINLPTRGIGKDS
                     LSKLEDYATINGLTLHDVLENIDSVPINITSNAKNGVLEFKRIIHKITDNWDKYGPAD
                     VISKIIKSIKYKEHLEKIHGNNEVAEERFENVGQLINMASSHMGKGKEAIKQFVEEVT
                     LMTDVVNDENANADVIKLMTIHSSKGLEFPYVFIAGVEENVFPLTNATMEAHLLEEER
                     RLMYVGVTRAKDYLFLSYANSRMTWGQVRNNPPSRFIGEIPEELTRHYDLGSGGNRNS
                     RADSYSSQDNFVSYYNTSRQEININEGDNVNHKLFGNGYVLEVRDHLAVVRFNDPKIG
                     TRKMEIRFLTVI"
     gene            2551..3108
                     /locus_tag="PK674_02565"
     CDS             2551..3108
                     /locus_tag="PK674_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15447.1"
                     /translation="MEKNNLKTRKKNRLDAFHYNDGYFFITVNVQNHEKVFGEVVDDK
                     MILNKYGEIVEKYRININKVHSNVSIGNFVIMPNHLHGILIMDNFDVDVREDNIFPKL
                     KQMSVINSAIQAGRSNRSYNKLSNIIKGFKQTTNKEIKKLGLKSFKRQKSFYDHIIRN
                     ENDMNRIIEYIELNPYAWENDEYYR"
     gene            3177..3542
                     /locus_tag="PK674_02570"
     CDS             3177..3542
                     /locus_tag="PK674_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="CoA-binding protein"
                     /protein_id="HOG15448.1"
                     /translation="MIDSKLTYAVIGASNNIEKYGYKVFKDFIDKGFNTIAINPNETE
                     ILGEICYSKLSDYDGKIDVAIFVLSPKFSLPVLEEIKELGIKKVWFQPGAESDEAIEF
                     CIRNGIEYVANACIMIQNR"
     gene            3773..4354
                     /locus_tag="PK674_02575"
     CDS             3773..4354
                     /locus_tag="PK674_02575"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="superoxide dismutase"
                     /protein_id="HOG15449.1"
                     /translation="MITLKKLPYEFNALEPFIDARTVEIHYTKHHQGYADKLNSLIEG
                     TEFESMDLEEIIMKSGGPIFNNAAQVWNHTFYWEGFIPNSKGIDGNTEISSAINHKRG
                     SFENFKTEFTNSAIGNFGSGWTWLVQNSNGELSIVNTSNAANPMTDGLIPLLTVDVWE
                     HAYYLKYQNRRTEYLENWWNLVNWNKVEELYGR"
     gene            complement(4498..5184)
                     /locus_tag="PK674_02580"
     CDS             complement(4498..5184)
                     /locus_tag="PK674_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15450.1"
                     /translation="MVPPLKRSFEFEITTAQQADSIVEALNEQERQKLLDALNKKMRT
                     NKEKKESQKKESKETLTKSLEFEEDTFKIDSKGWKEEKRKIYGEGKIKVKVNATGDVV
                     EYLEGLAKGEQIFITYDAFIREVMKAKNCSKEEVEQKYLMTDDELEEKMKDKPSNSEE
                     YKKFFTNEVKDHLAGFWGPRHEKFLAVGDRFNVWLAGGHSAVFMQNKWGLGSNNRFYG
                     FSGRLLKNKN"
BASE COUNT         2035 a          730 c          936 g         1742 t
ORIGIN      
        1 attttgtcaa atatggtttt gacaaaaaag caagtaaaaa aaatttttgt gattgaaaaa
       61 taattgggta tctataagtt ggtaaataat ttaattaatt ttgaaaaatg aatgcagaaa
      121 gtaaaagaat atttggcttg aaaattgaac gaagagcagt tgaaagcagc cttacatacc
      181 gatacttcta gtttaatttt agctggagca gggtctggta aaacaaggac tttgacatat
      241 aaaatatgat atttgatgtt tgggctaaaa atcaatccaa acaatatttt ggctgtaact
      301 tttacaaata aagcggccaa tgaaatgaaa gaaagactga tggagatctc tgacgatttt
      361 tctcagatga tgggggggac aatgtccgac gattgccatc aaaagatagg gacaatgtct
      421 gacgattttt ctcaaaagat atcagacgat aaatcgtctg atattgatga taatcgtcag
      481 acattacccc cagatgataa tcgtcagaca ttgtctccag aagacagtat tgatgatttt
      541 ttgaatttta tagaacaaac taaaccaagt ataggtggga ataatgtgaa atataccgaa
      601 aaagacttta aatggattgg gacatttcat agtatttttt tgaaaatatt aaaagaagat
      661 attgataaac tggagatgaa atatgataaa aattttacaa taatagactc aaatgatagc
      721 aacaaagtag taaaagatct tctgaaaaag cttaatttag atgaagtatt caaaccaaat
      781 gaggtaaaat gatttatcag taatcaaaaa aacaattgat atgatgccaa aatgtatcag
      841 tcaaaaatca ctacagatta tgagcaaaaa atggggaaag tttatgtgga ataccaaaaa
      901 gaattggaaa gatcaaatat gttggatttt gatgatttgt tgttgctacc ctatttgctt
      961 tttaggaaaa gagctgatat actagaaaaa tggaaaaaaa agtttttgta tattatggta
     1021 gatgaagctc aagacacaaa ctggatacaa tttgagctga tgatgatgct cagcggaaag
     1081 gatggaaata ttaccttgat atgagatgac taccaaagta tttatggccg gagatgagct
     1141 ttgatggaaa attttttgaa tgtaaaaaaa tattggccag atatccaaat gttcaaactt
     1201 caaatcaact atcgttctcg cccacatatt gtaaatgctg gaaactatat tataaaaaat
     1261 aatttgaaac aatacaataa agatgtgcat tctcatagaa aagaagatgg gaaaattact
     1321 gttttttgcc acaattctga tatagatgaa gctgctaaca taatagattt tattatgaaa
     1381 atgaaagata agggcagaat tcaaaaacta tgagatgttt cgatattata taggaccaat
     1441 gcccaatctt ctacttttga acaaatcttg gtgcaagaaa aaattcctta taaaatacga
     1501 tgagcatata aattttttga aagagaagaa gtaaaagata ttttggcata cattaaatat
     1561 ttagcaaata gtggagattc tgtttcctta aaaagaatta tcaacctacc tacaagatga
     1621 attggaaaag atagtttatc aaaattggaa gattatgcga ctatcaatgg attgacacta
     1681 catgatgttt tggaaaatat agatagtgtg cctataaata ttaccagtaa tgcaaaaaat
     1741 ggggttttgg aattcaaaag aataatacac aaaatcactg ataattggga caaatactga
     1801 cctgctgatg ttatttctaa aataataaaa tccataaaat ataaagaaca tttagaaaaa
     1861 attcattgaa ataatgaagt ggctgaagaa agatttgaaa atgtatgaca actaataaat
     1921 atggcatcaa gtcatatggg gaaatgaaaa gaagcaataa aacaatttgt agaagaagtg
     1981 actttgatga ctgatgtagt aaatgatgaa aatgcaaatg ctgatgtgat aaaacttatg
     2041 accatccata gtagtaaagg attagaattc ccttatgtat ttatagctgg tgtagaagaa
     2101 aatgtattcc ctctgacaaa tgctactatg gaagcacatt tattggagga agaaagaaga
     2161 ttaatgtatg ttggagttac tagagcaaaa gattatttgt ttttatctta cgcaaatagt
     2221 agaatgactt ggtgacaagt cagaaacaac cctcctagta gattcatttg agaaatccca
     2281 gaagaactta caagacatta tgatctatga tctggaggga atagaaattc aagagccgac
     2341 tcttattcca gtcaagataa ttttgtttct tattacaata caagtagaca agaaatcaat
     2401 ataaatgaat gagataatgt aaatcacaaa ttgtttggta atggttatgt tttggaagtg
     2461 cgggatcatt tggctgtagt gagattcaac gatccaaaaa taggaacaag aaagatggaa
     2521 ataagatttt tgacagtaat ataataaaaa atggagaaaa acaacttgaa aactcgcaaa
     2581 aaaaatagat tggacgcttt tcattacaat gattgatact tttttataac tgtaaatgtc
     2641 caaaatcatg aaaaagtttt tggagaagtc gttgatgata aaatgatttt aaataaatat
     2701 ggtgaaattg tagaaaaata tcgaataaac ataaacaaag tacattcaaa tgtgagtatc
     2761 tgaaattttg tgataatgcc taatcattta cactgaatat taataatgga taattttgat
     2821 gtggatgtaa gggaagataa tatcttcccg aagttaaaac aaatgagcgt gattaattcc
     2881 gctattcagg cgggacgcag taatcgctct tacaacaaat tatcaaatat tataaagtga
     2941 tttaaacaaa caacaaataa agaaattaaa aaattatgat taaaatcatt caaacgacaa
     3001 aaatcatttt atgatcacat aatccgtaat gaaaacgata tgaatagaat aattgaatac
     3061 attgaactga atccatatgc ttgggaaaat gatgaatatt atagataaaa ataactacta
     3121 accgctacta ttcggatagc acaacaatcg cacctacttt atttttaata acaacaatga
     3181 ttgattcaaa attaacttat gctgtgatat gagcatcaaa taatattgaa aaatatggat
     3241 acaaggtttt caaagatttt attgataaat gatttaatac aattgctatc aatccaaatg
     3301 aaactgaaat cttgggagaa atatgctata gcaagctatc agattatgat tgaaaaatag
     3361 atgtagcaat atttgtttta tctccaaaat tctctttacc agttttggaa gaaataaaag
     3421 aattaggaat caaaaaagta tggttccaac ctggtgctga aagtgatgaa gctattgaat
     3481 tttgtattag aaactgaatt gagtatgtgg caaatgcttg tattatgatt caaaataggt
     3541 agcctttgaa gttgcaagtt aaaagtagca ggtcaatggt tatctccctt actaagggct
     3601 aacactttat aaactttatg ataaaaaagt tcttcccttg ggggaagctg ctctatcgta
     3661 gttgggggta attgagagta aaaaaccccc acctagctac gcaaacatcc tcccccaagg
     3721 gaggaatttc gttctagaac cttataaacc tttacattat aaactataac aaatgatcac
     3781 attaaaaaaa ctaccttatg aatttaatgc tttggagcct tttattgatg caagaactgt
     3841 agaaatacat tatacaaaac accatcaatg atatgcagac aaattaaatt cattaataga
     3901 atgaacagag tttgaaagta tggatttgga agaaataata atgaaatccg gcggtccaat
     3961 attcaacaat gcagcccaag tatggaatca tactttttat tgggagtgat ttattccaaa
     4021 ctcaaaatga atagatggaa atacagaaat tagtagtgct ataaaccata aacgatgaag
     4081 ttttgaaaat ttcaaaacag agtttacaaa tagtgctata tgaaattttg gatctggatg
     4141 gacttggctt gtacaaaaca gtaatggaga attaagtatt gtaaacacta gcaatgctgc
     4201 aaatcctatg actgatggac ttattccctt attgaccgta gatgtttggg aacatgctta
     4261 ttatttgaaa tatcaaaata gaagaacaga atatttagaa aattggtgga atttagtgaa
     4321 ttggaataag gttgaggaat tgtatggtag gtaagctagt agtttatata aaaaatctct
     4381 cttgaggaga gatttttttg ttggtaattg ttgaaataat cacaccccgc tgaagcgggg
     4441 taaataatca aatagttgga tatttcggct attcgtgcct gcccgtctcc ggcgggtcta
     4501 gttcttgttc ttgagcagac gaccgctgaa accataaaat ctgttattac tgcccagacc
     4561 ccatttattc tgcatgaaga ctgcactgtg tccaccagct aaccacacat tgaatcgatc
     4621 tccgacagcg agaaattttt catgtctcgg accccagaag cccgcaagat gatccttgac
     4681 ctcattagta aagaattttt tatattcttc tgaattactt ggcttatctt tcattttctc
     4741 ttcaagttca tcgtctgtca tcaagtattt ctgttcaact tcctccttgc tacaattctt
     4801 agccttcatc acttctctga taaacgcatc ataggtaata aatatctgct ctcattttgc
     4861 tagtcattca agatattcta caacatctca tgtagcattt acttttactt ttatctttca
     4921 ttcgccatat atctttcttt tttcttcttt ccatcctttg ctatcgattt tgaatgtatc
     4981 ttcctcgaat tctaatgatt tagtaagtgt ttctttggat tccttctttt gggattcttt
     5041 tttctcttta ttagttctca tttttttatt caaagcatca agaagttttt gtctttcttg
     5101 ttcattaaga gcttcaacaa tactatcagc ctgttgagca gtagtaatct cgaactcaaa
     5161 agatcttttt agtggtggta ccatgatata ataattataa gataaaataa gttataacta
     5221 agtataatca gaatttttta ttttgcaagt cctgaagact ttttttgctg aatgcagaac
     5281 ttagtggttt ataagtacct ttttggttga ttgtttttat tgaatttagg gaattttttc
     5341 gtatttttca ttattttttc ataaaaaaaa tattttcaga taaaaatctg aaaaaaaact
     5401 tttcatttaa tattgaaata ttgtcgtgtt ttctttaata tga
//
LOCUS       DAOSID010000048         5417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_13530_length_5417_cov_11.182768, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000048 DAOSID010000000
VERSION     DAOSID010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13530_length_5417_cov_11.182768"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..489)
                     /locus_tag="PK674_02585"
     CDS             complement(202..489)
                     /locus_tag="PK674_02585"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF523 domain-containing protein"
                     /protein_id="HOG15451.1"
                     /translation="MSLTTPRSPAELKNGKVITKDGVDLTSQFEKGSQEVLKIAKAFN
                     CNEAILKARSPSCGVGKVYDGTFSGKLIDGDGVTAKLLKTNNIKVKTEDEI"
     gene            complement(480..614)
                     /locus_tag="PK674_02590"
     CDS             complement(480..614)
                     /locus_tag="PK674_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF523 domain-containing protein"
                     /protein_id="HOG15452.1"
                     /translation="MKIVSACLAGINCQWNGKSKPCEKVIELIKKGQAIPVCPEQLGV
                     "
     gene            complement(657..1160)
                     /locus_tag="PK674_02595"
     CDS             complement(657..1160)
                     /locus_tag="PK674_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15453.1"
                     /translation="MTQVKNQKKGLAVTSFVLSLVGLLLSITIVGAIIGIPIIILGLI
                     FGIIALAKKQKKGLAIAGTIISGLALLITSIFAFFFIRHYDEIVGPITEIAQMMKEDP
                     ELAVLMTNEQFAEEFQSKFQEVLIQKFGTEQLTNDGESVENLAVPTIMEEMKNVLYQL
                     KDEYYQK"
     gene            1331..1633
                     /locus_tag="PK674_02600"
     CDS             1331..1633
                     /locus_tag="PK674_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15454.1"
                     /translation="MSEHIISQTKLFVSGLPWKMRGMQLREEFEQFGEVVFARVVLDN
                     ETGRSRGFGFVEFVNPEDALKAKEAMNEKELGERLIRVDFAKENPDRIQNKEESEE"
     gene            2517..4970
                     /locus_tag="PK674_02605"
     CDS             2517..4970
                     /locus_tag="PK674_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="PKD domain-containing protein"
                     /protein_id="HOG15455.1"
                     /translation="MIKQNVQTIDNQDISDISNDPKILQAQQIQKILEQQEQIMAKYQ
                     ELKNLYENHQLDLSQKELVHQQMQKLNALYTQNKETLLKFGIGQDKANNESTKTSDKK
                     KKKKISFKLVLGLIGAGLVLMIGGVAFFLYSLLQNLDKVQKFGLTVDQAMLFLQIFVA
                     VFLGILIVISLLILSINLFRVIKKTYKKKIFSILGLIGGSILFLLILVFGFFTINVIK
                     NIDLDPSTQLVHGHLILKDGPKEIGLDPDLYIIAPVNIEFTLNYKVFNREITSNLGSP
                     KILGLSLDCGNGQILPINGDKFQGSCFYQTKGDYPIKLNVSYSDSQGQNNKDVSIRDV
                     VVRSQINISSNLGEIQTNNNELLVGKNPVNVIYDASDIFKEFQLDDYQIEWDFDGDGT
                     FDKTGLAYYMYIYTGVGVFYPGFKIPSLNNYIYSFPIRIEQSEAPVAYVNFDELEKGK
                     YNISAQFYGGSPVISEYKFNIIDKQTNSLVDSIFSKNSTIQYVFPGDGLYSVQIVFVT
                     ADGKQGSAESQNIDVGGSYFQIYYDVFTKTPTKSDFEKQDDLENIRITEVPTVIKVDI
                     KNIIPSVAGTNIKAFVNDSAVLIENNSFQTVIDSNEGAVIKIAVGDPNTDDFSEKEIN
                     VIVDRDDIIGSFLVTPDLVGISPFKVKFDASATTLTDPEDKIVYFSWDFGDGNKNENL
                     SHSIVEHTYTYDFATENGVFYPTVTLTTKKGRELTIGGGTRISVTKPIVNVEIVLDSH
                     PAQSANVGDRIEMSLNMDGSPTKIVWKFGDGRELECPGRSCTDVSQVYEVPGEYVIIA
                     SIFYENMPMVEGKINLVVK"
     gene            4997..5275
                     /locus_tag="PK674_02610"
     CDS             4997..5275
                     /locus_tag="PK674_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="aspartyl/glutamyl-tRNA amidotransferase subunit
                     C"
                     /protein_id="HOG15456.1"
                     /translation="MLDLEQLKHLQKLSNINLAQEQQEIFLGNLKGIIQMLDNLGKLD
                     LSNVNTSLNISNTLRTIKESKYFPYKKQILDNVEHELINNSVKIKGAI"
BASE COUNT         1924 a          624 c          877 g         1992 t
ORIGIN      
        1 caataagtat atatttttca atatatttcc tatagtagag gggtttgggg tgattgaagc
       61 cataagagag gttttttggg gagatttaga agaagaggga ttatttagaa taaaatagaa
      121 gttggagatg ttttttataa aaaaatagac acgaattaat tcgtgtctat aaaggtgttg
      181 tgattatgtt ataaaaagta actatatctc atcttctgtt tttactttta tgttgtttgt
      241 ttttagaagt tttgcagtta ctccgtctca atcaattagt tttcctgaaa aagttcaatc
      301 ataaactttt ccaacaccac aggatggaga tcttgccttt aaaatagctt cattgcaatt
      361 gaaagcttta gctattttta agacttcctg agatcatttt tcgaattgac tagttaaatc
      421 tactcaatct ttggttatta ctttaccatt ttttagttca gctggagatc taggagttgt
      481 taaactcata attgttcagg acatacagga attgcttgac ccttctttat taattcaatt
      541 actttttcac aaggttttga ttttcaattc cattgacaat ttattccagc caaacaggca
      601 cttacgattt tcatattcaa agatattcgt gtataaaagc atcatttctg atttctttat
      661 ttttgataat attcatcttt tagttggtat agtacatttt tcatttcttc cataattgta
      721 ggtactgcca agttttcaac actttctcaa tcatttgtca attgttcggt tcagaatttt
      781 tggattaata cttcttgaaa tttagattgg aattcttctg caaattgttc atttgtcatt
      841 aaaactgcta attctggatc ttctttcatc atttgagcta tttctgtgat tggtcataca
      901 atttcatcat agtgtcttat aaagaaaaat gcaaaaatgc tagttatcaa taatgctaat
      961 caagagatga ttgttcaagc aatagctaat catttttttt gttttttagc taatgcgata
     1021 attccaaaaa ttagtcataa aattataata gggattcaga tgattgctca aactattgta
     1081 atagaaagaa gtaatcaaac taacgaaagt acaaaacttg taacggcaag tcattttttt
     1141 tggtttttta cttgtgtcat tttagaaaat taaaatataa actgtttatt ttatatgaat
     1201 attcacttta aggtcaatag tagtttttga gttttttata acattaagat ttgttttttt
     1261 tattttctct tgtaatatga agagtaaaac ttatacattt cttgtatttt attttttttt
     1321 gtttaaatta atgtcagaac acattattag ccagacaaaa ttatttgtta gtggcttacc
     1381 atggaaaatg agatgaatgc aacttagaga agaatttgaa caattcggag aagttgtatt
     1441 tgcaagagtt gtattagaca acgaaactgg tagaagtaga ggattcggtt ttgtagaatt
     1501 tgtaaatcca gaagatgctt taaaagcaaa agaagctatg aacgaaaaag agctttgaga
     1561 aagattgatt agggtagatt ttgctaaaga aaatccggac agaatccaaa ataaagaaga
     1621 atctgaagaa taatatacta ttatatacca gaaataaaaa acttggacaa taattaggct
     1681 gtccaagttt tttgtatagc ggggagattg aatttactaa tatttggggt gttgaataat
     1741 ttgatatttt attattagta ttgatatgag taagttgata tatcttatta tttttgaaaa
     1801 ttgggtaaag tgtttagtat gttttggttt caatcggagg tttcgatggt aaaataagat
     1861 attttactta gaattgtttg gtcctacatt tcaagtatta ataaaatgta ttgaaaagta
     1921 ttttttaaaa tgtatcttgg gtgtaagact gagtggtgcg taggtgttac aataatattt
     1981 acatctcact aatatcatgg aagccgatta ttcttgatct aatcaagatg agctatccac
     2041 ctcctatttg ggagataaat aagtatcgtt tacggccact tggttttttt attttttgaa
     2101 atttttgttg gaaaagaaat ttactaatat ctgttatctg gaattttgag cattgttgat
     2161 atttaggata cgaaaaacta ttttttgggt taaaagcaaa tataaagatt caaacttagt
     2221 ttttatttcc ttatctgcat ttatagtaaa taatctggtg aatgtgtata taactatgtg
     2281 cgtttctctt attttctctt gaaaaacttg ggtaaattgt ttaaagtgtt gtaatattta
     2341 ctagaaggtt ggcttggaat aaaatatctt atttgtaatt atctggtaat gtattgtaaa
     2401 tcacaagtaa gtacttaact aaaataaact tgaattattg ttataaaacg atagaaggta
     2461 tgtggtagga ggcagaaaaa aaagaataaa ttctttagta atttaataaa aatataatga
     2521 taaaacaaaa tgttcaaact atagacaatc aagatatttc tgatatctca aatgatccaa
     2581 agattttgca ggctcagcaa atccaaaaaa ttttggaaca acaggaacaa ataatggcaa
     2641 aatatcaaga attgaaaaac ttatatgaaa atcatcagct tgatttatca caaaaagagc
     2701 ttgtgcatca gcaaatgcaa aaattgaatg cattgtatac tcaaaacaaa gaaactttac
     2761 ttaaattctg aatttgacaa gataaggcaa ataatgaaag cacaaaaaca tcagacaaaa
     2821 aaaagaaaaa aaagatttct tttaaattag tcttgggttt aatttgagca ggtttggttt
     2881 tgatgatatg atgagtggca ttttttcttt attctttatt acaaaattta gataaggttc
     2941 aaaaatttgg tctaacagta gatcaagcaa tgctgttttt gcaaatattt gtagctgtat
     3001 ttttatgaat acttatagtt atttcgctac ttattctatc tattaattta tttagagtaa
     3061 taaaaaaaac atacaaaaag aaaatatttt ctattttgtg attgatatga tgaagtattt
     3121 tgtttttgct tattttggtg ttttgatttt ttactattaa tgttattaaa aatattgatt
     3181 tagatccatc tacacaattg gtgcatggtc atttaatctt aaaagattga ccaaaagaaa
     3241 tttgacttga tcctgatttg tacattattg ccccagttaa cattgaattc actttaaatt
     3301 ataaagtatt caatagagaa attacttcaa atttatgaag tccaaaaatt ttatgacttt
     3361 ctctggattg ttgaaattga caaattttgc caataaatgg agataaattc caatgatctt
     3421 gtttttatca aacaaaatga gattatccaa taaaattgaa tgtaagttac agcgattccc
     3481 aatgacaaaa taataaagat gtttcgattc gagatgttgt tgttagatcg caaataaata
     3541 ttagttcaaa tttatgagaa attcaaacaa ataataatga attgcttgtt tgaaaaaatc
     3601 ccgtaaatgt tatatatgat gcatcagata tttttaaaga atttcaattg gatgattatc
     3661 aaatagagtg ggactttgat tgagattgaa cttttgataa aacatgactt gcctattata
     3721 tgtacatata tacgggtgtt ggtgttttct atcctggttt taaaatacct tctttgaata
     3781 attatattta ttcttttccc attagaatag agcaatctga agcccctgtc gcttatgtga
     3841 attttgatga attagaaaaa gggaaataca atatttcggc tcaattttat tgaggttctc
     3901 cagttatttc tgagtataaa tttaatatca tcgataaaca aacaaatagt ttggtagatt
     3961 caattttttc taaaaactca actattcaat atgttttccc tggtgatggc ttgtattctg
     4021 tccaaattgt ttttgttact gctgatggaa aacaagggtc agctgagagt caaaatatag
     4081 atgttggctg atcttatttt cagatatatt acgatgtttt tacaaaaact cccacaaaat
     4141 ctgattttga gaaacaggat gatttggaaa atataagaat tacagaagtc cctacagtta
     4201 taaaagtaga tatcaaaaac ataattccaa gtgtggctgg tacaaacata aaagcatttg
     4261 taaatgattc ggctgttttg atagaaaata actcttttca aactgttata gattcaaatg
     4321 agtgagctgt aattaaaatt gcggtttgag atccaaatac tgacgatttc tcagaaaaag
     4381 agataaatgt tattgtagat agagatgata ttatttgaag ttttttggtt actccggatc
     4441 ttgttggtat ttctccgttc aaggtaaaat tcgatgcttc tgctactaca ctcaccgatc
     4501 cagaagataa aatagtttat ttttcttggg atttttgaga ctgaaataaa aatgaaaatt
     4561 tatctcattc tattgtagaa catacatata catacgattt cgcaacagaa aattgagttt
     4621 tttatcctac ggttacttta acaacaaaaa aaggtcgtga attgactata tgatgatgaa
     4681 ctagaatttc tgttacaaaa ccgattgtaa atgtggaaat agtattagat tctcatcctg
     4741 cacaaagtgc taatgtttga gatagaatag aaatgtcttt aaacatggat ggttctccta
     4801 caaaaattgt atggaaattt ggagattgaa gggaattgga atgtccagga aggtcatgta
     4861 ctgatgtttc tcaggtgtat gaagtcccat gagaatatgt gattatagct tcgatatttt
     4921 atgaaaacat gcctatggtg gagtgaaaaa ttaatttagt agtgaaataa aaatcttagt
     4981 attttattaa aaaaacatgt tggatttaga acaattaaaa cacttacaaa aattatcaaa
     5041 tataaatctt gctcaagagc aacaagaaat ttttttggga aatttgaaat gaataattca
     5101 aatgttggat aatttgtgaa aattagatct atcgaatgtg aatacttcgt taaacatctc
     5161 aaacactctt agaactataa aagaatcaaa atattttcct tataaaaaac agattttgga
     5221 taatgtagag cacgagttaa taaataattc agtgaaaata aaaggtgcga tatagaagag
     5281 tttaaagttc aaagtttata aagttacaag ttataaagtt acaagtcaaa agttacttat
     5341 acgatagttt tacaaaaatt gttaggttaa ttttcaatca aaaagcacca ttttatagtt
     5401 aattataggt agtaatt
//
LOCUS       DAOSID010000049         5381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_13629_length_5381_cov_15.789335, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000049 DAOSID010000000
VERSION     DAOSID010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13629_length_5381_cov_15.789335"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..854
                     /locus_tag="PK674_02615"
     CDS             228..854
                     /locus_tag="PK674_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15457.1"
                     /translation="MKKLFFLAGFMLLVFFSLSACTKDVQDNLDDIAGTAKTNVQNTK
                     DYIAEKIALEEKLALLEVQKQKFSLPSRAKQLNITTPEGMTLDQNSSYQTSEEVEGFN
                     SMRFVYTGDYNLAMKQAEIIAQKAGVQISEEFKLAQDTLKKMGKNTLDQLKDMVGDLK
                     GVVYTNYSIIKNPETDQIISISVEEDGTLEIVVVDRKNMQNVVLDKIK"
     gene            884..2062
                     /locus_tag="PK674_02620"
     CDS             884..2062
                     /locus_tag="PK674_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HOG15458.1"
                     /translation="MKRFLFVIVFSLGFFLSFSFAQDYVYFYGNGCTHCAKVEKFFDE
                     NDVVSKYNVKELEIYFNRNNLKTFSDYIEKLEIDKNKAGVPFLVIDSENECDYVAGDK
                     KIIGFFQEKLTQDSSKVCAEGEDCSNFVCNDENCPHLNCGEIDDKDISGNVGGDGVDL
                     TSKWGRRKFFAIMLPAAFADSINPCAFAVMLLLLSSILTKTKSKRKAIISGFVFALAV
                     FLSYFAMGLGLFSALATATNTFVIKIIVGILGILVGLANLKDFFWYGKGFIMEVPLAW
                     RPKMQKVIKWAVSPLGVFIVGFLVSLFLLPCTSGPYFTILGYLASESKNLHTRGYIYL
                     IVYNIIFILPMVLIALLVGLGFKSAEELAELKSKNARLIHLIVGLLMLGLGMYVLLTM
                     "
     gene            complement(2154..2294)
                     /locus_tag="PK674_02625"
     CDS             complement(2154..2294)
                     /locus_tag="PK674_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15459.1"
                     /translation="MILYKKILYLPCFYILKIIKMADDDIILEEEDYDEEREDDDEDE
                     NY"
     gene            complement(2369..3133)
                     /locus_tag="PK674_02630"
     CDS             complement(2369..3133)
                     /locus_tag="PK674_02630"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOG15460.1"
                     /translation="MQDKWKVALEKFLKKWENKKEVTGILVCGSYVTGNPSKHSDIDI
                     HILLDSKTTRRERGNEIIDGFLIEYFANSTKRHHQYLEEDLKSRHKINAHMFCTGEIL
                     LDKTGELKRLVQYSQKYLKKKNPKLNKMQIEIAKYHIWDMCDNLEEVFDSNTEEFYLV
                     FYNFLNKLFETYAQFLQFDTVPIDKLRRFLVNEQDKKKYLVSDFPDKKFTKMIVSAIN
                     IKDKTKMMEEFKTITKYTLKKMGGFNIDGWKIKSPA"
     gene            complement(3757..5190)
                     /gene="gatB"
                     /locus_tag="PK674_02635"
     CDS             complement(3757..5190)
                     /gene="gatB"
                     /locus_tag="PK674_02635"
                     /inference="COORDINATES: protein motif:HMM:NF014930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOG15461.1"
                     /translation="MLLTIGLEIHIKLNSENKMFCQCKNEQNFDTLEPNTNICPVCTG
                     QPGALPTLSEDVLLLALKLGKALNCKINKESQFDRKSYFYPDLPMGYQITQLYKPTNT
                     NGEVSFFVNNYEEEKKIRILDAHMESDTAKMIHEGGQSLIDFNRAGTPLIEIVTGPDF
                     SNDDEVVEFLKELQRIVRYNNISNADMEKGQMRVDVNISIRKNESDPLGTRVELKNIN
                     SFGMIKRAILHEYNRQKTLLEKGEKVGQETRMRNDTEGISKMMRSKEDALDYRYFPEP
                     DMPILQLNDEILKNLDNEKLIIPHNIIKKFKQEYGFHKEYINALIGDFDTLLVFSELK
                     EQGLDPKNIAKWISGPINAYMKENFVGINDLPFNLKQLSEFIKISDEGKIMDNQMKIV
                     MDEMLKTGKSASEIIKEKGFDAPEISENDLENIAKKVLENNPTIVEQYKSGKTSTLGF
                     FVGQVMKETAGKANPKIVGEILEKLLK"
BASE COUNT         1717 a          733 c          799 g         2132 t
ORIGIN      
        1 tggaactttc agcaagatct gcttgttgta caacttggtt gtatcgttca ctgtggtttt
       61 taagtctact tgttaatttt ctcatctgtc tatttaagga taaaacttaa ttcacttata
      121 ataaaataaa aacaaatttc actacaaagt tgttcttttg ctcttgaaaa attttatgat
      181 attaatatac aattattagc acatttattt gtaattattt ttttgttatg aaaaaacttt
      241 tttttcttgc ttgatttatg ttgttagtat ttttttcttt gtccgcttgt acaaaagatg
      301 ttcaagataa tttagacgat attgcatgaa cagcaaaaac taatgtacaa aacacaaagg
      361 attacatagc agaaaaaata gctttggaag agaagctagc tttattagaa gttcaaaaac
      421 agaaattttc gcttccgtct cgagcaaaac aattgaatat tactacacca gagggtatga
      481 ctctagatca aaattcctct tatcagacta gtgaagaagt agagtgattt aactctatgc
      541 gttttgtgta tacttgagat tacaacttag ctatgaaaca agctgaaata attgcacaaa
      601 aagcatgagt acagattagt gaggagttta aattagctca agatacacta aaaaagatgt
      661 gaaagaatac attagatcaa ttaaaagata tggtttgaga tttaaaatga gtggtctata
      721 caaattattc tattataaaa aatccggaaa cagatcagat aatatctatt tctgttgaag
      781 aagactgaac ccttgaaata gttgttgttg atcgaaaaaa tatgcaaaat gttgttctgg
      841 ataaaataaa gtaaaaattt tatattttag atttgataaa aaaatgaaaa gatttttgtt
      901 tgtgattgtg tttagtttag gtttttttct ttcgttttct tttgctcaag attatgttta
      961 tttttatggg aattgatgta cccattgtgc caaggtagaa aaatttttcg atgaaaatga
     1021 tgttgtttca aagtataatg taaaagaatt ggaaatttat tttaatagaa ataatttgaa
     1081 aacattttca gattatattg aaaaacttga aatagataaa aataaagctt gagttccttt
     1141 tctagtgata gattcggaaa atgagtgtga ctatgtcgct ggagataaaa aaattatttg
     1201 attttttcag gaaaaattaa cacaagattc ctccaaggtt tgtgcagaat gagaagattg
     1261 ttccaatttt gtatgtaatg atgaaaattg tcctcattta aactgttgag aaatagatga
     1321 taaggatatt tcttgaaatg tttgatgaga tggtgtagat ttgacttcta aatggggtag
     1381 gcgaaaattt tttgcaataa tgctaccagc ggctttcgct gatagtatca atccttgtgc
     1441 ttttgcagta atgttattac tgctctcaag tattttaaca aaaactaaga gcaaaagaaa
     1501 agctattatt tctggatttg tatttgcact agctgttttt ttaagttatt ttgcaatggg
     1561 attaggacta ttttcagctc ttgctacagc tacaaatact tttgttataa aaataatagt
     1621 atgaatttta tgaatactag tatgattagc caatttaaaa gatttctttt ggtatggaaa
     1681 atgatttatt atggaagtac cgttagcttg gagacctaaa atgcaaaaag ttattaaatg
     1741 ggcagtatct ccattatgag tatttattgt ttgattttta gtgagtttgt tcttgcttcc
     1801 atgtacatct ggaccgtact ttactatact ttgatatttg gcgtcagaga gtaaaaatct
     1861 tcatactcga tgatatatat atttgattgt ctacaatata atatttattc tgcctatggt
     1921 attgattgct ttattagttt gattgtgatt taagtctgct gaggaactag cagaattaaa
     1981 aagtaaaaac gctagattga tacacttgat agtctgattg ttgatgcttg ggttgtgaat
     2041 gtatgtgtta ttgaccatgt aaaaaaattt gaaatattac aaaaatccag caaatgctgg
     2101 attttttgtt tactttgcat ttttaagata ctatgattgg tcgccattca ttactaataa
     2161 ttttcatcct catcatcatc ttctcgttct tcatcataat cttcttcttc caaaatgata
     2221 tcatcatcag ccatttttat tatttttaat atataaaaac aaggaagata taatattttt
     2281 ttatataaaa tcaagtaatt ttagttgttt tgaaaaacct tttcttaact gctatatcat
     2341 ttgaaaatat atttgagtgg tgtttaattt aggcagggct tttaattttc caaccatcaa
     2401 tattaaatcc acccattttt ttaagagtat attttgttat tgttttaaat tcttccatca
     2461 tctttgtttt atccttgata ttgatagcgg aaacaatcat tttagtaaat tttttatctg
     2521 gaaagtcgct aacgagatat tttttcttat cttgttcatt tacaagaaaa cgccttaatt
     2581 tatcaatagg tactgtatca aattgtaaaa actgtgcata agtttcaaat aacttgttca
     2641 gaaaattgta gaaaactaaa taaaactctt ctgtatttga atcaaagact tcttctaaat
     2701 tgtcgcacat atcccaaata tgatatttgg ctatttcaat ttgcatttta tttagttttg
     2761 gatttttctt ttttaaatat ttttgtgaat attgaacaag tctttttaat tcacctgttt
     2821 tatcaagaag tatttctcca gtacaaaaca tatgggcatt tattttatgt cttgatttta
     2881 aatcttcttc taagtattga tggtgtcttt tggttgaatt agcaaaatat tcaattaaaa
     2941 aaccgtcaat aatttcattt cccctttctc tccgagtagt ttttgaatct aaaagaatat
     3001 gaatatcaat atcagaatgc ttgctgggat tccctgtaac ataacttcca caaacaagaa
     3061 ttcatgttac ttctttttta ttttcccatt ttttgagaaa cttttctagt gcaactttcc
     3121 atttatcttg catttttata tgcattactt atataattca ttatcgtgtc tctatctgac
     3181 atatttcgca taatatataa atttttgtat atttcaatca aaacacttcg catagcgacg
     3241 agttagagga catacagatt tatcttactt ttttcgacaa aaaagtattg atttcttata
     3301 aaaaatattt attctacaaa tagttttcta ggaagaccta tcagggatag gtttaaggga
     3361 ctacaactaa tgtaacaagg tttccttgct tgcaagctcc cagaccaaca gctaaggcta
     3421 ctaaatgtag aagtgttgta attgtactgc cgaagctttg gagtcatgca aacattccag
     3481 aaacatatga tctgtaatga atgttaattc cttggaatat gactgttaat tccttggaat
     3541 atgactgtta atagaaaact atttttgaaa tagccttgat caatttgatc aggcttttct
     3601 tttttttaga gaaaacttgt aaaaaaaatt ataaaatata atctaaggaa agataaaact
     3661 atatgtaaat tctatggata ctctaacata cactatccta catattaaca tacgtcagat
     3721 tggtgtgaaa cattatgtgt catgtcccca attaacttat tttaaaagtt tttccaaaat
     3781 ttctccaact atttttggat tggcttttca agctgtttct ttcatgactt gtcatacaaa
     3841 aaatcataag gtgctagttt ttccggattt gtactgttct actattgttg gattgttttc
     3901 taggaccttt tttgctatat tttccaagtc attttcactt atttcaggtg catcaaatca
     3961 tttttctttg attatttcac tagcagattt tcaggttttt agcatttcgt ccattactat
     4021 tttcatctga ttgtccataa tttttcattc atcagaaatc tttataaatt cagataactg
     4081 ttttagatta aatggtaaat cgtttattca gacaaagttt tctttcatat aggcgtttat
     4141 tggaccagag atccatttag ctatgttttt gggatcaagt cattgttctt ttagttccga
     4201 aaatacaagc aaggtatcaa aatctccgat caaagcattt atatattctt tatggaatca
     4261 atattcttgt ttgaactttt tgataatatt gtgtgggata atcagcttct cattgtctaa
     4321 gttttttaaa atttcatcgt ttagttgtaa tatcggcata tctggctctg ggaaatatct
     4381 gtaatccaat gcgtcttctt ttgatctcat catcttgcta attcattctg tatcgtttcg
     4441 cattctggtt tcttgtcaga ccttttctca tttttctaat aaggtttttt gtctattgta
     4501 ttcgtgtaag atggctcttt ttatcattca aaatgaattt atatttttca attcaactct
     4561 tgttcacaaa ggatcgcttt cattttttct gatagaaata tttacatcta ctctcatttg
     4621 tcatttttcc atatcagcgt tggaaatgtt gttgtatcta acaattcttt gtagttcttt
     4681 taaaaattcc actacctcat catcatttga aaagtctggt caagtaacaa tttctattaa
     4741 aggagttcct gcacgattga aatctatcaa actttgtcat cattcgtgaa tcatttttgc
     4801 ggtatcactt tccatatgag catccaagat tcttatcttt ttttcttcct cataattgtt
     4861 tacaaagaaa ctgacttcac cattagtgtt tgttggtttg tatagttgag taatttgata
     4921 acccataggt aagtcaggat aaaaataaga ttttctatca aattgagatt ctttgtttat
     4981 tttgcaattt aatgcttttc caagttttaa tgctaatagt aaaacatctt cacttaatgt
     5041 gggtaatgcg ccaggttgac cagtacatac aggacagata tttgtgtttg gctctaatgt
     5101 atcaaagttt tgttcgtttt tacattgaca aaacattttg ttttcgctgt tgagtttgat
     5161 gtgaatttct aatcagattg ttagtagcat agtatatttg taagacataa attattaata
     5221 tttacttcaa gtaagatatt taattaagaa aaaaaaacaa gacatatctt ttgaaaatat
     5281 ttggaaattg tgtttggttt tattgttaaa ttaggcttat aaattgattt caaatccatc
     5341 atatgcaaat tgaaaatcaa catctgaata tttttctttc a
//
LOCUS       DAOSID010000050         5198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_14245_length_5198_cov_14.304686, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000050 DAOSID010000000
VERSION     DAOSID010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14245_length_5198_cov_14.304686"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PK674_02640"
     CDS             complement(<1..273)
                     /locus_tag="PK674_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15462.1"
                     /translation="MKTKKTLKIWKWILLILIIIFVRKVFCGPIKIDSTNPNSNIVIK
                     RGDNFYSFINGLPKTKQFALKIYIHFNKSSIDLSKIQEGTYVFSGKY"
     gene            589..2091
                     /locus_tag="PK674_02645"
     CDS             589..2091
                     /locus_tag="PK674_02645"
                     /inference="COORDINATES: protein motif:HMM:NF023495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HOG15463.1"
                     /translation="MNLITIVIIVVALGLGGLGGFFGYKKTLTEKAIKFKERMAKAKE
                     LEEEILEEARKKAEQILEKAEERADKLEEQRLKKVEEIQNKLLDREERMDEKLEKLEE
                     ERAKIIDKQKEAGKLVKEQTEKLAEIAKLNKEEAKKQLFANMEEEYQKEIKQFVEKMK
                     TIKTEEADKEAGQILAKVLPRIASESVNEFTVKSVDLPNEDYKGKLIGREGRNISFFE
                     KLTGVELIIDDTPMVVRISSFDNEKRFIAATVLKKLVKDGRINPHYIEKFYNQVVEEF
                     PNVLIEKGKEALTQLNLPMMNPEIVKYIGQLFLRYSYGQNIWLHSIEVAKIAEAIATE
                     IGLDPVMAKKAGLFHDIGKIVATTGQSHTVLGAELLKKLGMDDIIVNAAESHHYDVPM
                     THPISWVIAAADAISASREGARYDTSEMFIEKMSELEKLIKDISGIEKVHIMQAGREI
                     ITYVDPEQVSDKDLEKTLKTIGEKIEEQLDYPGIIRVTAIRENKLVEFLR"
     gene            2159..2947
                     /locus_tag="PK674_02650"
     CDS             2159..2947
                     /locus_tag="PK674_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15464.1"
                     /translation="MFPFFEVLGLKMYMTGIGIISFLLSFIIVAKYLCTKRHQDFYKI
                     FYWLPLAIAITYLFGSYSHFFLNHGAFPNSLEQLKLLFSPYGYHFHFTGLLIGFFISL
                     LIFFLKIKRYENKKIWIDIMFFSTVLSMIPLGIFLVFGDNFIGKASTSWIAIKPLTTQ
                     TELNKFGSVYPIGLFVSFLAIIDVIIILINKKRKKSFGQGMLGFIYFIIGLNIIFFFQ
                     QYPKYGVISLGGITFDIKTYVSFFAIMICLQLYYKRNQKSNTVV"
     gene            2970..4505
                     /locus_tag="PK674_02655"
     CDS             2970..4505
                     /locus_tag="PK674_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15465.1"
                     /translation="MKFFFVAEDSLYKIFKTLEKIPKGKNIEISIDSQHSLFDNERWG
                     KQIKEILDKKNIKAVFVTKTETTRRFFEKIGFKTKHIEKNKFMKYLRIIYLFFFDIKK
                     FHLNTFQNRKSYTFILVIGFEVLFVLGIMYLIYSLIIPGARIEINPSQQIETIIYNFR
                     YYPFEDQDYPRYSRFLSVPYYTGSLDYRYNMSMNASNVKFLQNPSVGEIKIFNKTEKQ
                     YALVPNTRFITEDGRLFQTTNRIEIPAGYQGVPGELIIRVKAMDKDDNDILMGDRGNI
                     AKGTTLYIKNLKQSLFLKEIYAVALEDFTGGTITSDGKVTQKDIDILSEKMVSYISQQ
                     KKNIVSQNFNIKDSLLLAFNDTISTTIKKIEVPHEVGENTSLLNGYVDISFSFFYIKW
                     SDLIRAFSEYIAQRPSNKTQLVSINKNSLVFFEEDGLTEKNSTFIIPTKIEITQGYDF
                     KKDVVGIVDDIKTYIVGGDKETARNYILGFPEVASVKIKITPAWYNSIPKLRSRIRVN
                     VQN"
     gene            complement(4502..4933)
                     /locus_tag="PK674_02660"
     CDS             complement(4502..4933)
                     /locus_tag="PK674_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15466.1"
                     /translation="MIETIKTSIFKKYKKDDIKGLFFSVFNEQGELLMSNGVVYTDKV
                     LEELIDLLYQGLIKKHTNIGSIVADVVLETVELKTPQEIQTISPKDFGIVLSDGSKSG
                     VLLPNTKGVNNIQEGLKLIKEKNDLGNNVQVVKFKTDRFII"
BASE COUNT         2010 a          648 c          775 g         1765 t
ORIGIN      
        1 atatttacca gaaaaaacat aagttcactc ttgtatttta gataaatcaa tagaactttt
       61 attaaagtgt atatatatct tcaaggcaaa ttgttttgtt tttggtaaac cgtttataaa
      121 ggaataaaag ttatctcatc ttttgataac tatatttgag ttcggatttg tagagtctat
      181 ttttatagga ccacaaaata cttttcgaac aaaaataatt atcaaaatta gtaagatcca
      241 tttccaaatt ttcaaagttt ttttggtttt cataagaaat tattaattgt aaaacgaaac
      301 ttatagtata aaagcaattt tattttgcaa taaaacatat gataaaatga taaagagatg
      361 aattgatgta ggggcgggtt atcaaccgcc cgttattaca ataaaaaaca ttcgtgataa
      421 agtgatgaaa tgataaaatg attttcttct tcctatccta ccacccgggt gcaatcggaa
      481 ttgttccact atttaatcac aaaaaatttc ccattgaaat taaaaaaata aatcatataa
      541 aaaagtgata agttttttgt ttttttaaaa tttaaaatta tagagataat gaatcttatt
      601 acaattgtga taatagttgt cgccctgtga ctttgagggc tttgaggctt ttttggatac
      661 aaaaaaaccc tcactgaaaa agcaataaag tttaaagaga gaatggcaaa agccaaagaa
      721 ctagaggagg aaattttaga agaagctagg aaaaaggccg aacaaattct tgaaaaagca
      781 gaagaaagag cagacaaact tgaagaacaa agattaaaaa aagtggagga aattcaaaac
      841 aaactgttgg atagagaaga aagaatggat gaaaaactag aaaaattaga agaagaaaga
      901 gcaaaaatta ttgataagca aaaagaggca ggaaagctcg taaaagaaca aacagagaag
      961 ttggcagaaa ttgcaaaact aaacaaagaa gaagcaaaaa aacaattatt tgcaaacatg
     1021 gaagaagaat accaaaaaga aatcaaacaa tttgtagaaa aaatgaaaac gatcaaaaca
     1081 gaagaagcag acaaagaagc tggtcaaatt ttagccaaag tattacccag aatagcctca
     1141 gaatcagtaa acgaatttac tgtaaaatcg gtagatctac caaatgaaga ttacaaatga
     1201 aaattaattg gtagagaatg aagaaatatt tcattttttg agaaattaac gggcgttgag
     1261 ttaattattg atgatactcc aatggttgtt agaatatcat cttttgacaa tgaaaagaga
     1321 tttattgctg ctactgttct aaaaaaatta gtaaaagatg ggagaatcaa tccccactac
     1381 atagaaaaat tttataatca agtagtagaa gaattcccaa atgttttgat agaaaaatga
     1441 aaagaagcat taactcagct aaatctccct atgatgaatc ctgaaatagt aaagtatatt
     1501 ggtcagttat ttttaagata cagttatggt caaaacatct ggctacatag catagaggtt
     1561 gcaaaaattg ctgaagcaat tgctacagaa ataggattgg atcctgttat ggcaaaaaaa
     1621 gcatgacttt tccatgatat tggaaaaatt gttgctacaa catgacaatc tcatacagtt
     1681 ttatgagcag aattactaaa aaaacttggt atggacgata tcattgtaaa tgctgctgaa
     1741 tctcatcact atgatgttcc tatgacacat ccaatatcat gggttattgc tgcagcagat
     1801 gcaatttctg catctagaga atgagctaga tatgatacaa gcgaaatgtt catagagaaa
     1861 atgtcagaat tggaaaaact tatcaaagat atttctggta ttgaaaaagt tcatattatg
     1921 caagcaggta gagaaattat aacttatgta gatccagaac aagtatcaga taaggatttg
     1981 gaaaaaacat taaaaactat ttgagaaaaa atagaagaac agttagatta tcctggtatt
     2041 attagagtta ccgctattag agaaaataaa ttggtagagt ttttacgcta gtagcccaaa
     2101 aataaaaatt tattaaaact tcttcttggt caacttttta ttttcaacaa gctattccat
     2161 gtttccgttt tttgaggttt tgtgattaaa aatgtacatg acttgaatat gaatcatctc
     2221 ctttttgtta tcatttatta tagttgcaaa atatctttgt accaagcgac accaagattt
     2281 ttacaaaata ttttattggt tacccttagc tatagctatt acatatttat tttgatctta
     2341 ttctcatttt tttctcaatc attgagcatt cccaaatagt ttagaacaat taaaattatt
     2401 atttagtcct tattgatatc atttccattt tacctgatta ttaatcggtt tttttatttc
     2461 attacttatc tttttcttaa aaataaaaag atatgaaaac aaaaaaatat ggattgatat
     2521 aatgtttttt tctaccgttt tatctatgat accacttggt atattccttg tatttggaga
     2581 taactttata ggaaaagctt ccacatcttg gatagcaatc aaacctttga ctacacaaac
     2641 agaattaaac aaatttggat ctgtttatcc tattggacta tttgtatcgt tcttagctat
     2701 tattgatgtc ataatcatat taattaataa aaaaagaaaa aaaagttttt gacaatgaat
     2761 gttgtgattt atatatttta taataggact caatattatt ttcttttttc aacagtaccc
     2821 taagtatggt gtaatatcct tgtgatgaat cacttttgat ataaaaactt atgtttcatt
     2881 ttttgcaata atgatatgtt tgcaattata ttataaacga aatcaaaaat caaatactgt
     2941 agtttaacaa ataacctaaa atataaaata tgaagttttt ttttgtggct gaagatagtt
     3001 tatacaaaat ttttaaaacc ttagaaaaaa ttccaaagtg aaaaaatata gaaatatcta
     3061 tagattcaca acattctttg tttgataatg aacgatggtg aaaacaaata aaagaaattt
     3121 tggataaaaa aaacattaag gctgtttttg ttacaaaaac agaaacaact agaagatttt
     3181 ttgaaaaaat ttgatttaaa acaaaacata tagaaaaaaa caaatttatg aaatatttaa
     3241 gaataattta tttgtttttt tttgatataa aaaagtttca cttaaatact tttcaaaaca
     3301 ggaaaagtta tactttcata ttggtgattt gatttgaagt tttgtttgtg ctttgaataa
     3361 tgtatttaat ttattcatta attattccag gtgctagaat agaaataaat ccgtctcaac
     3421 agatagaaac tataatatat aattttcgtt attatccatt tgaggatcaa gactatcctc
     3481 gttattctag atttctcagt gttccatatt atacatgatc actagattat aggtataaca
     3541 tgtctatgaa tgctagtaat gtgaaatttt tacaaaatcc atcggtttga gaaataaaaa
     3601 tttttaataa aacagagaaa caatatgcat tagttccaaa tactaggttt attactgaag
     3661 atggaagact atttcaaaca acaaaccgaa ttgaaatacc agctggatat caatgagtcc
     3721 cttgagagct tattattagg gtaaaagcaa tggataaaga tgataatgat atattgatgt
     3781 gagataggtg aaatatagct aaatgaacaa ctttgtatat aaaaaaccta aaacaaagcc
     3841 tatttttaaa agagatttat gctgtagctt tggaagattt tacatgatga actataacaa
     3901 gtgattgaaa agtaactcaa aaagatatag atatattatc tgaaaagatg gtttcttata
     3961 tttctcaaca gaaaaaaaat attgtatcac aaaactttaa tataaaggat tctttattat
     4021 tagcttttaa tgacactatt agtactacta taaaaaagat tgaagttcca catgaggttt
     4081 gagaaaatac ctcactatta aatggatatg tagacattag ttttagtttt ttttacataa
     4141 agtggtcaga cttaattaga gcgttttccg aatatattgc tcaaagacca tccaataaaa
     4201 cacaattggt tagtataaac aaaaactcat tggtgttttt tgaggaagat tgattgactg
     4261 aaaagaacag tacttttatt attccaacta aaatagaaat tactcaatga tatgacttta
     4321 aaaaagatgt tgttggaata gtagatgata ttaaaactta tatagtttga tgagataaag
     4381 agactgctag aaactatatt ttatgattcc cagaagttgc tagtgtaaag ataaaaatca
     4441 ctcctgcttg gtacaatagt ataccaaaac taagatctag aataagggta aatgttcaaa
     4501 attaaattat aaacctatct gttttgaatt taacaacttg aacattattt cataaatcat
     4561 ttttttcttt tattaacttt aatcattctt gtatattgtt tactcacttt gtatttggta
     4621 acaatactcc agatttgctt caatcactta atacgattca aaaatctttt ggagatatgg
     4681 tttgtatttc ttgtggagtt ttaagctcta ctgtttctaa aactacatct gctactatgc
     4741 ttccaatatt tgtgtgtttt tttatcaaac cttgatataa taagtctatt agttcttcca
     4801 aaactttatc agtataaact actcaattgg acatcagcaa ttctcattgc tcattaaaca
     4861 cagaaaaaaa tagtcatttg atgtcatctt ttttatactt tttgaaaatg gaagttttta
     4921 ttgtttcgat cattaacagt agttaaaagt taaaggttgg gatcaacttc ccgaaccccg
     4981 atttatcggg gcaaagggaa gaacttcgtg gtatatagtt tctcttgata gatatgtaga
     5041 tatataggcg cgaattcgaa ttcgcgcctg caataactta cgggcgaact tttcactata
     5101 tagttcctct tgatagtttt tgtggtttat atagtatata tactataatt tttaacattt
     5161 tactatcaac ttattttatg tctaaactga aatttaaa
//
LOCUS       DAOSID010000051         5158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_14407_length_5158_cov_16.171272, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000051 DAOSID010000000
VERSION     DAOSID010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14407_length_5158_cov_16.171272"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2714)
                     /locus_tag="PK674_02665"
     CDS             complement(<1..2714)
                     /locus_tag="PK674_02665"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF559 domain-containing protein"
                     /protein_id="HOG15467.1"
                     /translation="MNEYQNLVLEIIELNSQNKVTLDEFKNIKRMFSKKHKLSDIPTN
                     IKLIRAYHQLLKAKKISKNIDIENLFKKRSIRSDSGIVAVQVLTKPYPCPGQCIFCPN
                     EKGMPKSYISTEPGAMRAALNQFDPIRQVYNRLLSLNMTGHQTDKIEMIVLGGTWDFY
                     PKDYKIEFIKSLYDACNTFCQLDINIPDEEGSLGNSAVSQILPNPPLILNPPSTPFSD
                     SKSSPNPLLGKRRGLYTSFSPLFQRGDRGDLEKIEGDRGDLELKRGIEGVIENKKDEN
                     FSTISSSTQGGSKIEEEENGLVSKYRYLSVKGEVGLFDFHGNESYIREQGFITNGTLP
                     YHKDAREKAKILRKNMTKAEEKIRKHFLKDLDINVYKQKPIDHFIADFYIPGSKLIIE
                     VDGLIHSKPDNFEYDVRRDDILSLYGIVVARIKNEDIENNFDGVKEELIDLIEQTKSI
                     KEQIDKENSSFSFNGKDFGLIGEKENENDLDQQAIKTKRYGYNITNLDTIKYSSSIQE
                     AITINETANNRIIGLTVETRPEFITDENCRFWRELGVTRLEIGVQSLYDDVLEANKRG
                     HSVQQIRQGIHKLRQYGFKFSIHIMPGLYQSDYEKDLGTFEKTYSDPFIKPDEIKFYP
                     TSVIPNTELYDLYISGHYKPLLIEHIQKLIKETFKKIIPPYTRIKRLIRDIPSHEIAA
                     GSNITNLSQLTHNELLEEFRNNSESAKKLYSRLYGNYQLFDSFEEFITFCLKQKNLNL
                     ENGNINTYIIGQDPDLEIYRKFVCLDTRSREIRHKPKCKNSQEKDSVNLIIRQYESSA
                     GIEYFLSFEDLQGYIYGFTRLLLPKESETIDWEGLGKQTALIRELHIYGQLASLKENI
                     KSSYKKQHKGFGSQIMFIAEQISKSFGYQRLSVISGVGVRGFFKKIGY"
     gene            2832..>3760
                     /locus_tag="PK674_02670"
     CDS             2832..>3760
                     /locus_tag="PK674_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOG15468.1"
                     /translation="MKLLITCFQGTAVALANELKILGYQIFDNFSSGMYVNGKMEDVY
                     KINLNSRIANKVYLEVGEKIVKNFDQLFEFVQNIDRKKFIRKGHKVSISTNIFQSQIN
                     SNKSTQSIVNKAILKKLTGGDGHWLSDPQKEPISILVQIINDKCSIFVNTSGESLHNR
                     GYRKETGEAPLKENLAASLIRMCGRHFKTPLLDPMCGSGTICIEAAMIAKNIAPGLQR
                     NFAFETFEDFDQNKFDEIKKELKLKIFEGKYQITGYDIDPKVLELAKNNAKRAGVLDT
                     ITFEQRNIKDLERQNDYIVCNPPYGKRLKDDDI"
     assembly_gap    3761..3860
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3870..4418)
                     /locus_tag="PK674_02675"
     CDS             complement(3870..4418)
                     /locus_tag="PK674_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="AAA family ATPase"
                     /protein_id="HOG15469.1"
                     /translation="MIIGITGTLGAGKGTISDYLIEKKGFKHYSVSGYITEEIKKRGM
                     EVNRDTMYQVGRGLKRENGADFIVKELYKKAKTEGGKAIIESIRSPGEVDGLKELDDF
                     KLISVDADIKTRYERIKLRGSEKDNVDYETFVANNAREWDSDSTDPTKINLSKCVLQA
                     DFKIDNNGSFQDLYDQVDEIVS"
     gene            complement(4592..5134)
                     /locus_tag="PK674_02680"
     CDS             complement(4592..5134)
                     /locus_tag="PK674_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOG15470.1"
                     /translation="MDILRITLSIAVLILGFIGIFLPVLPGPPLAYLSLIFIQIANKP
                     FSTQFLIVMAVVCVFLMIMDYAIPIIGTKKMGGTKRGVNGSTIGLVLGVVILPIMGVV
                     IGPFGLIGLLGGPFLGAYVGEIVYQKYKKQKLDNKKALKSAFGSFLGFISGVLIKIIY
                     TIIVAIYLFPKIFGLIKGML"
BASE COUNT         1753 a          801 c          686 g         1818 t
ORIGIN      
        1 ctatatcaaa tttttttgaa aaatcatcta actccaactc atgaaataac agacaacctt
       61 tgatatcaga aacttttcga aatttgttct gcaatgaaca ttatttgaga accaaatcac
      121 ttatgttgtt ttttgtatga tgatttaata ttttctttca aacttgcaag ttgtcagtaa
      181 atatgtaact ctctaataag agccgtttgt tttcataatc attcccaatc aatagtttca
      241 gattcttttg gcaatagtag tctagtaaaa ccataaatat atcactgtaa atcttcaaaa
      301 gaaagaaagt attcaatacc agcagaactt tcgtactgtc taattataag atttacggaa
      361 tctttttctt gtgaattttt gcattttggt ttatgtctga tctctctact acgagtatcc
      421 aaacaaacaa attttctata aatttccaag tcaggatcct gtcaaatgat gtatgtatta
      481 atatttcaat tttctagatt aagatttttc tgtttcaaac aaaatgtaat aaattcttca
      541 aatgaatcaa ataattgata atttcaatac aacctagaat atagtttctt tgcgctttca
      601 gagttatttc taaattcttc caacaactca ttatgcgtaa gttgagaaag atttgtaatg
      661 tttgatcatg cagcaatttc atgagatgga atatcacgaa tcaacctctt aatcctcgta
      721 taaggaggaa taattttttt gaaagtttct ttaattaatt tttgaatatg ctctataagc
      781 aaaggtttgt aatgtcagga gatatataag tcataaagtt cagtatttgg aatcactgat
      841 gttggataaa atttaatttc atctggcttt ataaagggat ctgaataagt cttttcaaaa
      901 gttcataaat ctttttcata atcagattga tatagtcaag gcataatatg gatagagaac
      961 ttgaatccat attgtctcaa tttatgaatt cattgacgaa tttgttgaac agaatgtcat
     1021 cttttattag cttccaaaac atcatcatac aaagattgaa ctcaaatttc cagcctagta
     1081 actcacaatt cacgccaaaa tctacaattt tcatcagtaa taaactctgg cctagtttct
     1141 acggtaagac ctataattct attgtttgca gtttcgttta ttgtgatcgc ttcttgaatt
     1201 gaggaagaat attttatcgt atccaagttt gtgatattat atcagtatct cttggttttt
     1261 attgcttgtt gatctagatc attctcgttc tctttctccc caattaatca gaagtctttt
     1321 caattgaaag aaaaggaaga gttttcttta tcaatttgtt cttttatact ttttgtctgt
     1381 tctattaaat caataagctc ctctttaact caatcaaaat tattttctat atcttcattc
     1441 tttattcttg caacaacaat tcaatacagg ctcaatatat catctcttcg aacatcgtat
     1501 tcaaaattat caggtttaga atggattaat caatcaacct ctattataag ttttgatcaa
     1561 ggaatataaa aatctgctat aaaatgatct ataggtttct gtttatacac atttatatcc
     1621 aaatctttca aaaaatgttt tcgtattttt tcttcagctt ttgtcatatt ttttctaaga
     1681 atcttagctt tttctcttgc atccttatga taaggcaaag tcccatttgt aatgaaaccc
     1741 tgttcacgaa tatatgattc atttcaatga aaatcaaaca gtcaaacttc tcattttaca
     1801 gataaatatc tatatttgct tactaaacca ttctcctctt cttctatttt agatcacccc
     1861 tgagtggaag aagagattgt agaaaagttt tcatcctttt tattttcaat caccccctca
     1921 atccccctct ttaattctaa atcaccccta tccccctcta ttttttctaa atcaccccta
     1981 tcccctcttt gaaaaagagg ggaaaagctt gtgtagagcc cccttctttt tcaaagaagg
     2041 gggttggggg atgatttaga atcagaaaat ggggttgagg gaggatttaa gatcaaagga
     2101 gggttaggga ggatttgaga aacagcggag tttccaaggg atcattcctc atctggtata
     2161 tttatatcca actgacaaaa agtattacaa gcatcataca aagattttat aaattctatc
     2221 ttataatcct ttggataaaa gtcccaagtt catcataata ctatcatctc aattttatct
     2281 gtttgatgac ctgtcatgtt taacgaaagc aatctgttgt aaacttgtct tataggatca
     2341 aattgattga gtgcagctct cattgctcca ggttctgtac ttatataact tttgggcatt
     2401 catttttcgt ttggacaaaa aatacactgc ccagggcatg gatatggttt tgtaagaact
     2461 tgaacagcaa cgataccaga atctgaacga attgatcttt ttttgaaaag attttctata
     2521 tcgatatttt ttgaaatttt ttttgctttt agaagttgat gatatgcacg aattagtttt
     2581 atatttgtag gaatatctga taatttatgt ttcttagaaa acatcctctt gatgtttttg
     2641 aattcatcta gagtaacctt gttttgggag tttaattcta ttatttccaa tactagattt
     2701 tgatattcgt tcatttattt ttagtgtaaa ataagtcgta tcaataacga atattacttt
     2761 aattaagaaa gctttgtttt caagaagaaa aaaactaaaa tgattttgat tttttagaaa
     2821 aaatagaaaa aatgaaatta ctaatcacat gttttcaatg aacagctgta gctttagcca
     2881 atgaactcaa aattttatga tatcaaattt ttgataattt tagtagtgga atgtatgtaa
     2941 attgaaaaat ggaagatgtt tataaaatca atcttaattc tagaatcgca aataaagttt
     3001 atctagaggt aggagaaaaa atagtaaaaa attttgatca attgtttgag tttgtacaga
     3061 atatagaccg aaagaaattt ataagaaaat gacataaagt cagcatctct acaaacattt
     3121 ttcaatcaca aataaatagt aataagtcca cacaaagtat tgtaaataag gctattttga
     3181 aaaaattaac ttgatgagac tgacattggc tatccgatcc ccaaaaagaa ccaatcagta
     3241 ttcttgttca aataataaac gacaaatgta gtatttttgt taatacaagt tgagaatctt
     3301 tacacaacag atgatataga aaagaaactg gcgaagctcc attaaaagaa aatttagctg
     3361 ctagtttaat tagaatgtgt ggtcggcatt tcaagactcc cctactggat ccaatgtgct
     3421 gatctggtac aatatgtatt gaagcagcaa tgatagcaaa aaacatcgct cctggacttc
     3481 agagaaactt tgcttttgaa acttttgaag attttgatca aaataaattt gatgaaataa
     3541 aaaaagaatt aaaattaaaa atctttgaat gaaaatatca aataacttga tacgacattg
     3601 atccaaaagt tttagaatta gcaaaaaaca atgctaaaag agctggagtt ttagatacaa
     3661 tcacatttga acaaagaaac ataaaagatt tagagagaca aaacgactat atagtttgca
     3721 atcctcctta tgggaaaaga ctaaaagacg atgatatcaa nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn aactactcac taactcacta tctcatcaac ttgatcatac
     3901 aagtcctgaa aacttcaatt gttatcaatc ttgaaatctg cctgcaaaac acattttgat
     3961 aaatttattt ttgtaggatc tgtactatca ctatcccatt ctcttgcatt gttagcaaca
     4021 aaagtttcat aatctacatt atctttttca cttcaacgta acttaattct ttcatatcta
     4081 gtttttatat ctgcatctac agagattaac ttaaaatcat ctaattcctt gagtcaatct
     4141 acttctcatg gagatctaat actttctata attgcttttc atcattctgt tttagctttt
     4201 ttgtataatt cttttactat aaaatctgct caattttctc ttttgagtcc tcgtcatact
     4261 tggtacatag tatctctatt aacttccatt catctttttt taatttcctc agtaatatag
     4321 ccactaacag aataatgctt aaatcatttt ttttctatca aatagtctga aattgttcat
     4381 tttcctgcac caagtgttca tgtaattcaa ataatcatga ctcattagtt aaaagttaaa
     4441 agtaacaatt tagttataaa gttacaagtt tataaagtta ttaatacttg atgaacttta
     4501 agactgaaga cattcttccc ttgggggaag ctgagcccga tgaatcggga agaacaacct
     4561 cccccaaggg aggaactacc aactttacaa actacagcat gcctttgatt agtccaaata
     4621 ttttgggaaa caaatatatt gctacaatga ttgtataaat aattttaatt aaaactccag
     4681 atataaatca caaaaaagat caaaatgccg attttaatgc ttttttattg tctagttttt
     4741 gctttttata cttttgataa actatttctc atacataagc tcacaaaaaa ggtcatcata
     4801 acaatcaaat taacccaaat ggtcaaatga ctactcacat aatcggcaat attacaactc
     4861 ataggactaa tcatattgta cttcaattta ctcatcgctt tgttcatcac atttttttag
     4921 ttcaaattat tggtatagca taatccatta tcataagaaa aacacaaaca acagccataa
     4981 caattaggaa ttgtgtagaa aatggtttgt ttgctatctg tataaaaatc aaagacaaat
     5041 atgccaaagg tggtcatggc aaaactggca aaaaaatccc tataaatcat agtattaaaa
     5101 cagctattga aagtgttatt cgtagtatat ccattctaag aagttgtaat gattaaat
//
LOCUS       DAOSID010000052         5082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_14681_length_5082_cov_11.523374, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000052 DAOSID010000000
VERSION     DAOSID010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14681_length_5082_cov_11.523374"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PK674_02685"
     CDS             complement(<1..284)
                     /locus_tag="PK674_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOG15471.1"
                     /translation="MKKTLTIDKTKRICWQTPEQDDIEKLSKQYNFHEMIKENMLDIN
                     AESKFSTIDDNFFMALAFTKYLKSKSKYVFNELDIVIGKDYIITTTGLFS"
     gene            complement(345..2105)
                     /locus_tag="PK674_02690"
     CDS             complement(345..2105)
                     /locus_tag="PK674_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15472.1"
                     /translation="MTGIKHHLNLTSRNIGHHFKKHHKKYVFGLISGALLYKTLSLIL
                     ANVFLNNFGTHQSFADNTEIPTNSEVEYVEENNSNDNSSGENLYYQNNEEENNLSGDS
                     GTENSTPEQEDPEPTNEIDPEVCSLGDIELLSPLSGDILKGEIEISWIYKNKICEDIP
                     LIIKLRDANTQYIKIGEANEGETGLNFDSSLLYSGYYNITGLNLSGEVIITHTGEYTG
                     LNTKYFSGHKIVIYSSDMEVLCEGEKFTIDNEAPSLSNVEVNTDTISGNYIGLGKKVS
                     ISFGASEELSGVSVTILGVNADLEEKENNNYEYISYLSEQHTSGNIIYNIEFEDLAGN
                     TGYHEGYSNIIFDKDYPEVTKLEFSKTGDNIKLILNTDKSTSLEFVYVLSGNNTGYSI
                     NSSFGTSHNMLLENIRTGSYYNYSVAIKSLANNVLYIGGYFRISGDNVSYDFQNISGS
                     NMLVGVGFMPKTINSGSVLDSLLIEIDKFNSCKQGIPKFTNINIPIGLYHTQIKMPEL
                     EKSYVKKLVSAFSIVLFERIEDAGLNKEIVEKITEEFNDFLVILKLVQDDDNQCEQSL
                     SNYYMSRFKNNLIKYNLADE"
     gene            2356..2973
                     /locus_tag="PK674_02695"
     CDS             2356..2973
                     /locus_tag="PK674_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15473.1"
                     /translation="MKKIKNSILVLLLAGLGSTFGQTTTLFIENRDGYVETIVANKFL
                     LEIQQDLSKIYGTTSQQWEACQNPGKRIFIGKKPFERKIKWENPIFFGVSVEGLNKDL
                     SIRYDGKNLTNEELLSPYSSRRSSIKEDGIGILVPILIALFYAISRAVSDLKRRKKKE
                     KIYLSLDVVVLTALCFLVYCLGLGIIYGLIVGFGLIMIPTLVYRL"
     gene            complement(3197..4567)
                     /locus_tag="PK674_02700"
     CDS             complement(3197..4567)
                     /locus_tag="PK674_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG15474.1"
                     /translation="MEGNKNKRKIKRLCFALIWGMIHMVSLGIAVEGNKTFAANMPFI
                     TFQETQKENSTKIKESISDVLFKFVDFIKTKVSKNNPKHTSSSNITIDNSYVEEKFLP
                     KEYFLDVLYNKDKFYINFLAKEKIVSTSQEKFYPDNFIRLNELLKILVNSYRYKIGYN
                     LDSNIGLSNKNYFDKLMPKYYNTAYEMGLLNGLENIDDYERFVSYDDLNQILQNFKRQ
                     YPKLINLYYLDIDQSNITIKRGDICRIVFKTLMLDNDKYIDIAYQDIYYHTNFDAIQN
                     LASLGITNIENSRFYPDDNISRGDFIVMFVRSYLKKTNKTLEVSKIDFDIEDLDYNSI
                     YAPYVLYAKEHGLVDYLLEIVRAKKYINLEKFITKHEAYYIVSNIANVSFDYDILKAD
                     KEFISRGESAQLMVDAFEFSSKSGASKNNLDRDNTPNSQLEKFVINIKGLIKSPKSSW
                     ILDQAS"
BASE COUNT         1751 a          687 c          669 g         1975 t
ORIGIN      
        1 tctgaaaaca atcatgtagt ggtaataata taatcttttc aaataactat atccagttca
       61 ttaaaaacat atttggattt agattttaaa tattttgtaa aagctaatgc cataaaaaaa
      121 ttatcatcta tagtgctaaa tttagattca gcattaatgt ctagcatatt ttcctttatc
      181 atctcatgaa aattatattg ctttgataat ttttcaatat cgtcttgttc tggagtttgc
      241 caacaaatcc gttttgtttt atctattgtt aatgtttttt tcatattaaa taattaaatt
      301 atactaaaat tgttataaat cccgtttttt gtggacaata tctgttattc atctgctaaa
      361 ttatacttta ttaaattatt tttaaatctt gacatatagt aattcgataa actttgttca
      421 cattggttat catcatcctg aactaatttt aatataacaa ggaaatcatt aaactcttct
      481 gtgatttttt ctacaatttc tttatttagt catgcatctt caattctttc aaaaagcact
      541 atggaaaaag cactaactag tttttttaca taagatttct ccaattctgg catttttatt
      601 tgtgtatgat ataatccgat tggaatattt atgttagtaa attttggtat tcattgttta
      661 catgaattaa atttatctat ttctattaat agactatcta acacagatca agagtttatt
      721 gttttaggca taaatcaaac acccacgagc atatttgatc aactaatatt ttgaaagtca
      781 tacgacacat tatctcagct aattctaaaa tatcctcaaa tatataaaac attgttggcc
      841 agacttttaa ttgccactga gtaattataa taggatcagg tccttatatt ttctaataac
      901 atattgtgag aagttcaaaa ggacgaattt atactatatc aagtattatt tcacgataac
      961 acataaacaa attccaaact ggtgctttta tctgtattta aaatcaattt tatattgtct
     1021 caagttttac taaactcaag ttttgttact tctggataat ccttatcaaa aataatattt
     1081 gaatatcatt catgatatca tgtattacca gctagatctt caaactcaat attatatata
     1141 atatttcaag aagtatgttg ttctgacaga tagcttatgt attcataatt attgttttct
     1201 ttttcttcta aatctgcatt tactcacaat atggtaacac ttactcatga taattcttca
     1261 ctagcaccaa aagatattga tacttttttg cctaatcaaa tgtaattccc acttatagta
     1321 tccgtgttta cttctacatt agataaactt ggtgcttcat tatctattgt aaatttttct
     1381 cattcacata atacttccat atcagaggaa tatatcacta ttttatgtca tgaaaagtat
     1441 tttgtattca atcatgtata ttctcaagtg tgagttataa ttacttcacc acttaaattt
     1501 agtcatgtta tattataata tcacgaatat aatagtgaac tatcaaaatt taatcaagtt
     1561 tctcattcat tagcttctca aatttttata tattgagtat ttgcatctcg gagttttata
     1621 ataagcggta tatcctcgca aatcttgttt ttatagatcc aagaaatctc aatttctcat
     1681 tttaaaatat ctcacgataa aggggacaat aactctatat cccctaaact acaaacttct
     1741 gggtctattt cattggtagg ctctgggtct tcttgttctg gagttgaatt ttctgttcca
     1801 gagtctcctg ataaattatt ttcttcttca ttattttgat aatataaatt ttcacctgaa
     1861 gaattatcat ttgaattatt ttcttcaaca tattccactt ctgaatttgt tggtatttcc
     1921 gtattatctg cgaaagattg atgtgtacca aaattgttta aaaaaacatt agctaaaatt
     1981 aatgataatg ttttatataa caaagcacca gaaattagtc caaaaacata ctttttgtga
     2041 tgttttttga aatggtgtca gatgtttcta gaagttaaat ttagatgatg tttgattcat
     2101 gtcattttgt ttactataat ataataaata tattttttat attataagta taaccgaaaa
     2161 atgttttatt gtcaaatttt tgagtacttt ttttaaaaga gatgtttgac ttttatgaaa
     2221 aaaatctaat aataggctag gaaagtataa aaaaatataa ttaagtaaat acaatccata
     2281 ttttttacaa ttgactttgt taacaaaata gttattgtta ttaacataac aaaaaacaat
     2341 ataaaattaa aaattatgaa aaaaataaaa aattcaattt tagttctgct tttagcagga
     2401 cttggtagca catttggaca aactaccact ctatttattg aaaatagaga tggttatgta
     2461 gaaacaattg tggctaataa attcttacta gaaatccagc aggatttgag taagatttat
     2521 ggaacaactt cccaacaatg ggaagcatgc caaaatcctg ggaaaaggat ttttataggg
     2581 aaaaaaccat ttgaaagaaa aattaaatgg gaaaatccca tttttttcgg tgtctcagta
     2641 gagggactaa ataaagatct ctctattagg tacgatggga aaaatcttac caatgaagaa
     2701 ttgctatctc cgtactcatc gcggagatct tcaataaaag aggatggtat aggaattttg
     2761 gtaccgatac ttattgcttt attttatgca ataagtaggg ctgtttcaga tttaaaaagg
     2821 agaaagaaaa aagaaaaaat ttatctttct ctagatgtgg ttgttctgac agctctgtgc
     2881 tttctagtct attgtctagg attaggtatt atttatgggc ttattgttgg atttgggtta
     2941 ataatgatac ctacattggt atataggctg tagcaaaaaa ttaaaaatca ataaaaatca
     3001 ataataaata gacgaaatag cactttgttg ttttaacggg tgctattttt tttctaaaaa
     3061 acttatcaac aaaggataat ttaaaattta aaatagcttt tagggatgat ttaaatttca
     3121 tgaaaatgga gatttgaaca aaaaaagatg gaaattttgt tgttgaaata tcgtataaaa
     3181 tagtataata aagtttttaa gatgcttggt ccaaaatcca agatgatttt ggagatttta
     3241 ttagtcattt aatgtttata acaaattttt ctaactgact atttggagtg ttatctctat
     3301 ctaaattatt ttttgatgct catgatttgg aagagaactc gaaagcatcc accataagtt
     3361 gtgcagattc tcatctagaa ataaattctt tatctgcttt caaaatgtcg taatcaaaac
     3421 taacattagc gatatttgat actatataat atgcttcgtg ttttgtaata aacttttcaa
     3481 gattaatata ttttttagcc ctaacaattt ccaaaaggta gtctacaaga ccatgttctt
     3541 ttgcataaag aacatagggt gcgtaaattg aattgtaatc caaatcttct atatcaaaat
     3601 caatctttga tacttccaag gtcttgtttg tcttttttaa gtaagacctg acaaacatta
     3661 ctataaaatc tcaacgagat atattatcat ctgggtaaaa tctagaattt tcaatattag
     3721 taattcacaa agatgccaaa ttttgaattg catcaaaatt tgtatgataa taaatatctt
     3781 ggtaagctat atctatatat ttatcgttat ccaacattaa agtcttgaat actattctac
     3841 aaatatctcc tcttttgata gtaatgttcg attgatcaat atccagataa tataaattta
     3901 tcaattttgg atattgtctt ttgaagtttt gtaggatttg gtttaagtca tcataagaaa
     3961 caaatctttc ataatcatct atattttcca atcaatttaa taatcacatt tcgtatgcag
     4021 tattatagta ttttggcata agtttatcaa aatagttttt attagataaa ccaatgtttg
     4081 aatctaggtt atatcctatt ttgtaacgat aggagttcac taaaattttt aacaattcat
     4141 ttaagcggat aaagttatct ggataaaact tttcttggct tgtggaaaca attttttctt
     4201 ttgctaaaaa atttatgtaa aatttatctt tattatataa aacatccaaa aagtattctt
     4261 tgggaagaaa tttttcctct acataactat tatctatagt aatgtttgat gaagatgtgt
     4321 gtttagggtt attcttcgat acttttgttt ttataaagtc tacaaattta aaaagaacat
     4381 ctgatattga ttctttgatt ttggtagaat tttctttttg ggtttcctga aatgtgataa
     4441 atggcatatt tgctgcaaaa gttttattac cttctacagc tatacctaaa cttaccatgt
     4501 gaatcattcc ccaaattaaa gcgaaacaca gtcgttttat ttttcttttg tttttatttc
     4561 cttccattta ttttaaatta taatttaaac ggacgctgta attatatatc ttttttttga
     4621 tttgtcaagt caatttgtta atttttaaaa acacttaatt atagtccaga atttagtcag
     4681 ataaagactg gtcgtttaag taaaaaagtt aataaagtta aaagttcata aagttaaaag
     4741 ttgttaataa aacattctta ccttgcgaag caaaatcgag aactctcttt ttccccattg
     4801 cttgtaggcg cgaattcgaa ttcgcgcctg ccaaaaaacc aggaatgttt gtttttaatc
     4861 actttaacac tttatcgcga acgaagttca ccccgcttaa tggagcgagc atttatcatt
     4921 ttaacatttt gtcgcgaact ccgtgagcat ttatcatttt atcgcgagcg gagcgagctt
     4981 ggtatattat ctttattttc aaaattattt tgttagtatg ataagacatt taatttttaa
     5041 ttattctttc ttatgaaata cgaaataaaa aaaatcaaat ga
//
LOCUS       DAOSID010000053         4996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_15009_length_4996_cov_9.638737, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000053 DAOSID010000000
VERSION     DAOSID010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15009_length_4996_cov_9.638737"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PK674_02705"
     CDS             <1..330
                     /locus_tag="PK674_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15475.1"
                     /translation="VILSGNLVLSIRQKSSPNVTWEGTGTLLDANGDELAANFAKAIP
                     GILKFNFTQITDVADRTISSKGEITYRVEINANRFSIDELSIILKSIQFVYDDDGTPS
                     AQITKKW"
     gene            518..1354
                     /locus_tag="PK674_02710"
     CDS             518..1354
                     /locus_tag="PK674_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15476.1"
                     /translation="MIGNEVILFDSKSIRKEFVNGERRFSVVDIVSVLTNSQGKDKGA
                     YWRKLKQRLIAEGSEVVTNCHELKMLASDGKKYKTDASNTKGILRIIQSIPSPNAEPL
                     KQRLASLGNERMEELSNPELGINRAKARAVMIRKKQGKDDRWITSRLQSIQTRNSFTD
                     ILKERGIKDGFEYALLTNKVYSVGLGVSGGATEYRSIKGIDKKDNLRDHMDNLEIALV
                     DLAEAGSQKIIETKGSHGFDEVQDAVVTGSGIAGEAREKIENQIGKPIVSPKNYKLKD
                     NT"
     gene            1432..1935
                     /locus_tag="PK674_02715"
     CDS             1432..1935
                     /locus_tag="PK674_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="transposase"
                     /protein_id="HOG15477.1"
                     /translation="MPRRKPFLKNQYYHVYNRGLQKSTLFFEFRDFQRFLFYIGKWME
                     EFRDSIGIVAYSILPNHFHFVILNKEEGLQLSNFMSRLTSSYAKYMGTKYGSQTGLRF
                     FESRFKAKLLDNQEYLDQCIHYVEFNPVKHALTDNIENRLFTSYDKNSKKASPNQEIL
                     ALDWEFV"
     gene            1972..2475
                     /locus_tag="PK674_02720"
     CDS             1972..2475
                     /locus_tag="PK674_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="NYN domain-containing protein"
                     /protein_id="HOG15478.1"
                     /translation="MEKNFVFIDTQNINLSIQDQGWKLDWKKFNKYLQKKYKAVKIYL
                     FIGYVPENQSMYTFFQNCGYTLIFKPVLELNNGQTKGNIDAELVLQAMIDYKKYDKAV
                     IITGDGDFACLVKYFYKKNKLLMLIVPNERKYSIFLRKTAKEKIDSLTNLRTKLEYKK
                     KVAPTKQ"
     gene            2869..3108
                     /locus_tag="PK674_02725"
     CDS             2869..3108
                     /locus_tag="PK674_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOG15479.1"
                     /translation="MFTNYIHSLLDQTVYYKDEDGVVIAKIPNKPGYFTQGDSFEDAR
                     GNMINLIETLMIDDIKNGKIDLKKDFTMNKEFGYA"
     gene            3101..3319
                     /locus_tag="PK674_02730"
     CDS             3101..3319
                     /locus_tag="PK674_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOG15480.1"
                     /translation="MPKVTPINARELVKKLRKLGFEGPYVGGKHMFMMKDGYRVPFPN
                     HGGKDISRGVVNCIIEYIGISVDERNSL"
     gene            complement(3618..4172)
                     /locus_tag="PK674_02735"
     CDS             complement(3618..4172)
                     /locus_tag="PK674_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15481.1"
                     /translation="MKKLALFVLLYFFVFFAIVLAQAQTTKEVQLTVLKYGLVIGTPA
                     NLNLGNVTAGSQIEKTFDGHFWVEDFRKGYGTGYYTTIQCDGLYGPNDFVITGMLLKG
                     NSIEILEGDEGEEELIHSYLDSWKDITQPQLYFYKNSSTPQSSNGRKYGSKPSINVSI
                     PSDAPAGDYKGRITYTLYDMSFDS"
     gene            4181..4309
                     /locus_tag="PK674_02740"
     CDS             4181..4309
                     /locus_tag="PK674_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15482.1"
                     /translation="MNFLKSKKNNQYNGYGLFLKKQMFLCKKSKFGIDKEQEMAYT"
     gene            complement(4441..>4996)
                     /locus_tag="PK674_02745"
     CDS             complement(4441..>4996)
                     /locus_tag="PK674_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15483.1"
                     /translation="KKIYKLGALIVALFALGFITLSVQDAQAADKEVSLSITAGSNTC
                     TFGNYNPMTSPASYTTGVVVSNQPSYECTLLAGQQDGGQANTVTVQSTNLTGSWTGAT
                     HCIPAANVKLKHVNAPIVRSGACQITTYLSTGYTNVASTQTLIKKDELTICTIGSSTQ
                     IQITIPASLPVGTYSGYMIFTEPA"
BASE COUNT         1836 a          670 c          880 g         1610 t
ORIGIN      
        1 gttattttgt ctggaaatct tgttctttct atacgacaaa agtcaagtcc gaacgttaca
       61 tgggaatgaa catgaacatt attagatgct aattgagatg aattagctgc taattttgca
      121 aaagctattc catgaatatt aaaatttaac tttacacaaa ttacagatgt tgctgataga
      181 acaataagtt ctaaatgaga aataacatat cgtgtagaaa tcaatgctaa tagattctct
      241 atagatgaat tatctatcat attaaaatca atacaatttg tttatgatga cgatggaaca
      301 ccatctgctc aaataacaaa gaaatggtag aataaattct caaaaaaacc ttccttcggg
      361 gaggtttttt ttgttgctag aaaattgaca ccggggttcc agtggagcgg agaataaccc
      421 cggggtcagt aatgttttta atttgtcaaa aataatattg attttataaa gaatattatt
      481 atcatgaaga tatatttata aattaattat tcgaaaaatg atatgaaatg aagtaatatt
      541 atttgatagt aaatcaatta gaaaagaatt tgttaatggt gaacgacggt tttcggttgt
      601 agatattgtt tcagtactga caaatagtca atgaaaagat aagtgagctt attggagaaa
      661 acttaaacaa agactgattg ctgaatgaag tgaggtcgtg acaaattgtc acgaactgaa
      721 aatgttggca tctgatggga aaaaatacaa aacagatgct tcaaatacaa agtgaatttt
      781 gcgtattatc caatcgattc cttctccaaa tgcagaacca cttaaacaac gactagcttc
      841 acttggaaat gaaaggatgg aagaattaag caatccagaa ctttgaatta atagagcaaa
      901 agctagagct gttatgatac ggaaaaaaca atgaaaagat gatagatgga taacttctag
      961 attgcagagt attcaaacta gaaattcatt tacagacatt ttaaaagaaa gatgaattaa
     1021 agattgattt gagtatgcac tattaaccaa caaagtgtac agcgtttgat tgtgagttag
     1081 tggttgagca acagagtata gaagtataaa atgaatagat aaaaaagata atcttagaga
     1141 tcatatggat aatttagaaa tagcgttagt tgatttagcg gaagcttgat ctcaaaaaat
     1201 cattgaaaca aaaggaagtc attgatttga tgaagttcaa gatgctgtgg taacatgatc
     1261 gtgaattgct tgagaggcta gagaaaaaat tgagaatcaa atttgaaagc caatagtgtc
     1321 accaaaaaac tataaattaa aagataatac ttagagaata agaataaagc tgaccccggg
     1381 acactttggg ggtcagtaat aagtagatat ttatatttta accatagaag aatgccaaga
     1441 agaaagccat ttttgaagaa tcagtattat catgtttaca acagatgatt acagaaaagt
     1501 acattatttt ttgaatttag agactttcaa agatttttgt tttatatttg aaagtggatg
     1561 gaagaattta gagacagtat atgaattgta gcctattcca ttctgccaaa ccatttccat
     1621 tttgtaatct tgaacaaaga agaatgattg caactatcaa attttatgtc cagactcaca
     1681 tctagctatg cgaaatatat gtgaacaaaa tatggatcac aaacatgatt gagatttttt
     1741 gaatctagat tcaaggcgaa gttgctagat aatcaggagt acttagatca atgtattcat
     1801 tatgttgagt tcaatcctgt aaaacatgct ttgaccgata atattgaaaa tcgacttttt
     1861 actagctacg ataaaaattc caaaaaagct tctcctaatc aggagatttt ggcgttggat
     1921 tgggagtttg tgtagagata aagaagtatt tatttgataa tagaataaaa gatggaaaag
     1981 aactttgtgt ttattgatac tcaaaatata aatttatcaa tacaagatca gtgatggaaa
     2041 ttagattgga agaagtttaa taaatatctt cagaaaaaat ataaagcagt aaaaatttat
     2101 ctttttatat gatatgttcc agaaaaccaa agtatgtata cattcttcca aaattgttga
     2161 tatactctaa tattcaagcc tgttcttgaa ctaaataatt gacaaacaaa atgaaatata
     2221 gatgcggagc tggttcttca agccatgata gattataaaa aatatgataa ggcggtcatt
     2281 attacatgag atggtgattt cgcttgtttg gtgaaatatt tttataaaaa aaataagtta
     2341 ttaatgttaa tagttccaaa tgaaagaaag tattctattt tccttagaaa aacagctaaa
     2401 gagaaaatag attctttaac taatttaaga acaaaattgg aatacaaaaa aaaggttgca
     2461 ccaaccaagc aataattact tgatccatac aaccccatcg tgataacaac atcattatag
     2521 ctaaaaaaaa ttaaattgca aataaaaatg gagatttttc tgtcatctgt tgaaaagtgt
     2581 tattttggtg gtggtatgtg gggaaaggaa aaagagtggg ggaaagaaaa gttcctccct
     2641 tgggggaggt tgtttgtgta gctaggtggg ggttttaccc ccaactacat tagaacagct
     2701 tcccccgagg gaaggatttc tttgtctccg tgcgttcact tcgttcactt agtcgaaatg
     2761 acaaatacaa ctgaaatttg ttcattgatt caaagattta ttttcagctt gaaaaaatag
     2821 cttttatgtt tataattgaa ctagttttat ccagtaacta atgggaaaat gttcacaaac
     2881 tatattcatt ccttattaga ccaaacagta tattacaaag atgaagattg agtggttatt
     2941 gcaaaaatcc caaataaacc atgatatttt actcaatgag atagttttga ggatgcaaga
     3001 tgaaatatga tcaatttaat agaaacattg atgatagatg atattaaaaa tgggaaaata
     3061 gacttaaaaa aggattttac tatgaataaa gagtttggtt atgcctaaag ttacacctat
     3121 taatgctaga gaattggtga agaaattaag aaaactttga tttgaatgac cttatgtgtg
     3181 atgaaaacat atgtttatga tgaaagatgg atatagagtt ccattcccaa atcattgatg
     3241 aaaagatatt tcaagatgag ttgtaaattg tataattgaa tatatctgaa tttcagtaga
     3301 tgaacgaaat agcctataag agtcttcctt tggggagatt tttttgtttt ttataagtct
     3361 atagatttat ttatattgca agaaatggga ggatgttaaa tccctctatc cccgctattc
     3421 agcgggggag accagctccc tttgataaag ggagacattt tgctgtttct cctacctgta
     3481 gcttaaaaat ttccacaagc ggggttgcaa gaacagcacc ttagaataaa aaaatttgat
     3541 taaagcatac aaaaaatgct atataaaaaa aatcccgaaa tttttttcgg atttttttat
     3601 gatttatgtc tagtcaatta ggaatcaaaa ctcatatcat agagtgtgta tgtgattctt
     3661 catttataat caccagctgg cgcatcactt ggtatggaaa cattgatgga tggcttgctt
     3721 caatatttcc gaccattaga gctttgtgga gtactgctat ttttgtagaa atagagttgt
     3781 ggctgggtaa tatctttcca agaatccaaa taggagtgta tcaactcctc ttctcattca
     3841 tctcattcta gaatctctat agaatttcat ttcaatagca ttcaggtaat cacaaaatca
     3901 ttgggtcaat atagtccatc acattggatt gtggtgtaat acccagtccc atatcatttt
     3961 ctaaaatctt ctacccaaaa atgaccatca aaagtctttt ctatctggga tcatgcggta
     4021 acatttccta aatttagatt ggcaggagtt caaatcacta agccgtattt caaaacagtt
     4081 agctgaacct cttttgttgt ttgggcttga gccaagacga tagcaaaaaa aacaaagaag
     4141 tataagagta caaataaagc cagttttttc atcacaaaaa atgaattttt taaaatcaaa
     4201 aaaaaataat caatacaatt gatattgatt atttctcaaa aagcaaatgt ttttatgtaa
     4261 aaagtctaag tttgggatag ataaagaaca agaaatggca tacacataga gttttatatt
     4321 ttcaaataac gaacgatgcc ataatttaga ttggctatat tatattatgt ggcctaggat
     4381 aaaataatct aaactattag atttttatta acctagactt ttgtttgttt ttttattaaa
     4441 ttaagcaggc tctgtaaata tcatatatcc actataagtt cctactggta agcttgcagg
     4501 aattgtaatt tgtatttgag ttgaagagcc aatagtacat attgtaagct catctttttt
     4561 tatcaaagtc tgtgtacttg caacatttgt atatccagtt gataagtaag tagttatttg
     4621 gcaagctcat gacctaacta ttggagcgtt aacatgctta agttttacat ttgcagcagg
     4681 aatacaatgt gttgctccag tccaagaacc tgttagatta gttgattgaa cagttactgt
     4741 attagcttgt catcaatctt gttgacctgc tagtaaagta cattcgtaac ttggttgatt
     4801 agacaccaca actccagtag tataagaagc tggagatgtc attggattat aattaccaaa
     4861 agtacaagta ttacttcctg ctgtaatact taagctaacc tctttatcag cagcttgagc
     4921 atcttgaaca cttaatgtta taaaaccaag tgcaaaaaga gccactataa gggctcataa
     4981 tttgtatatt tttttc
//
LOCUS       DAOSID010000054         4965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_15128_length_4965_cov_12.663544, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000054 DAOSID010000000
VERSION     DAOSID010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15128_length_4965_cov_12.663544"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..623)
                     /locus_tag="PK674_02750"
     CDS             complement(39..623)
                     /locus_tag="PK674_02750"
                     /inference="COORDINATES: protein motif:HMM:NF015347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HOG15484.1"
                     /translation="MMKKSFLIIFFLLLTFIPISAFAGWQDPIHSGNFVTTWQTDIAG
                     ATEPTKITLRFLKSETDICSVSWNCDGNFETFSGNTIIHDYGTTGTYDVCIKSDYPLA
                     FYGFGLQNNEKAKLLEIKQRGDIQWSSFNSAFKGMINMQLTAEDLPDLSAVTDMSSAF
                     EGATNFIGHASMNSWNTSGVTNMASMFRGAVQVT"
     gene            complement(735..1958)
                     /locus_tag="PK674_02755"
     CDS             complement(735..1958)
                     /locus_tag="PK674_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15485.1"
                     /translation="MKTILKWISVIVVAVAFFCIGIIFIPMEKQIVGKMELTYIFVDQ
                     YGNEYDIFAPVHGAATDWSFLFDDNKSVEQNPIVNVNANNMDDESPTKTDYLMDVVDH
                     EVDEEENLEEKKWLEERKLEEKRLEEERLEETLDGTNTGLDNTELDIDVGGAEIKEGN
                     VDGLVKGLDEIRKDVEKDQKTCVTPWKTVLKHGESVIAYEQRKDVPTICNAQRRTCNN
                     GVLNGTYLQGACDEGVEYKYTKVKVISYNTKTPGELIQNPKYAKYDGAEFDTRGKINP
                     DTKIPYTDWDNYVKDGPIKDPNINLGGKNKMHYNCVSPWGDSIQHGQFIRAYKSPIGF
                     TDELCQVELRLCLDGNLNGSFVNKSCEYVGVSSQDYREGNVDVTKPSQELLDEVSQKD
                     PNRKGFRGRLKGRFG"
     gene            complement(2196..>4965)
                     /locus_tag="PK674_02760"
     CDS             complement(2196..>4965)
                     /locus_tag="PK674_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=25
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HOG15486.1"
                     /translation="NNHRMVYMKVLVPRGDSKIDREQAKELAKDMKEKIGRMSQVYLN
                     FHKIGRLSMKDKIMYRIFRKPRIVLMYHYEGGILNFIVGCFPEYQKILEGAIGAQFAD
                     SALEVVKRPNIFRKKYHKVMPLQSEKSSIYTIRLYKRMMDDPINNIIDSMNNISKSDT
                     ATVIMTIKPLSDKWNEKAKRQVDRLYKNLDLRPSGFFPAIWYGIKTFFKLLFHGEIPV
                     KQKEENISMVRMVKAKEETINDMAEEIGTVAFESGLILVTSSDHEDKLKNNIQNLVSS
                     YNVYTDQYGNELEQLGNRATIFGFLARPLWRIAASYNLTNLFYNSNTFTLNELTSLYH
                     FPDYTYNRSNIIERMQYKILPAPSNLPSFRDGKESGFIMSGIVAEKYKGGKLSEILKE
                     YKDHRAVGSRKVIEEKLVPIENFKQNELAGKEIEEKDGKKFVKVEEEKEIYGYKIYKD
                     GVLLGTNIYRNTYSPVYMKRNDRTRHHYCIGKSGTGKSVFLQTLARQDIWNGDGVCVI
                     DPHGDLVEDMLEYIPKERAKDVIYFDAGDEERPMGLNLYEIDNIDEADRIVNDATEIF
                     IKMFGPEIFGPRIQEYFKYGSLTIMEDFEDRPTILDVVRLYTDEAFREYKVAKVKNAV
                     VRNFWEKTYNAMGDREKSEIIPYFSSKFVSFNTNRLIRNIIGQTKSAFRLNDVMDEGK
                     ILLINLSKGKIGDMNAQLLGMILVSKIYTAAMARGKIPEKDRKDFYLYVDEFQNFVSG
                     TFADILSEARKYRLCLIMAHQYIAQLEAGQGLNRSEGGKGDVKAAVFGNVGTMMSFKV
                     GAPDAEFLEKEYQPVLGMGDITGIANYKAYIKLNIDNSTTRPFSMNSIYTGDYKNKKI
                     VPILKEYSAKKYGRKRKFVDAEISARLGLNLEEVDKKQSEGLEIDIQEMKKNQKNKEN
                     QGPVDVSS"
BASE COUNT         1613 a          958 c          532 g         1862 t
ORIGIN      
        1 caatctcctg ttaattttac aaaaaatttg ttaccgaatt aagtaacttg tacagccccc
       61 ctaaacatac tagccatatt agttacccca ctcgtattcc aactattcat agatgcatgg
      121 ccaataaaat ttgtagcacc ctcaaaagca gaagacatat cggtaaccgc cgataaatca
      181 ggaagatcct cagcagttaa ttgcatgttt atcataccct taaaagcact gttgaaactt
      241 gaccactgga tatctccccg ttgtttaatt tctaaaagct tagctttttc attattctgt
      301 aatccaaaac cataaaaagc taaaggatag tctgatttga tacaaacatc ataggtgccc
      361 gtggtcccat aatcatgaat aattgtgttc ccagaaaatg tttcaaaatt accatcacaa
      421 ttccaagaaa cagaacaaat gtctgtttct gatttcaaaa atctcaaagt aattttagtc
      481 ggctcagttg ctcaagcaat atcagtttgc caagtagtta caaaattgcc tgaatggatg
      541 ggatcttgcc accctgcaaa tgctgaaatg gggataaagg ttaatagtaa aaagaaaata
      601 attaaaaaac ttttcttcat cattttacaa aattaactaa aaaattcccg aatcgtaaaa
      661 tattaccctc tttttataaa ttacagtttt aattacaaca gaaaatcttt caattataat
      721 tatactaaaa atttttatcc aaaccgtccc ttcaatcgcc ctcgaaatca ttttctattt
      781 ggatcttttt gtgaaacctc atccaacagt tcttgactag gtttagttac atccacattt
      841 cattctctat aatcttgaga agacactcat acatattcac aacttttgtt tacaaaactt
      901 cagtttaaat ttcaatccaa acaaagcctt agctctactt gacacaattc gtctgtaaat
      961 caaatagggc ttttgtatgc tcttataaac tgtcaatgtt gtatggagtc tccccatgga
     1021 ctcacgcaat tatagtgcat tttattttta cctcataaat ttatattagg atcttttata
     1081 ggtcaatctt ttacataatt atcccaatca gtgtaaggaa tttttgtatc cggatttatc
     1141 tttccacgag tatcaaattc ggctcaatca tattttgcat atttaggatt ttgaattaac
     1201 tcccccggag ttttggtatt ataagaaatt acttttactt tggtgtattt atattcaact
     1261 ccttcatcac aagctcattg caaatatgtt caattcaaaa ctccgttgtt acaagttctt
     1321 ctttgtgcat tacatatagt tgggacatcc tttctttgtt cataagcaat tacggactct
     1381 ccatgtttca aaacagtttt ccaaggggta acgcaagttt tctgatcttt ttcaacatct
     1441 tttctaattt catctagccc ctttactaaa ccatcaacat ttcactcctt aatttctgcc
     1501 cctcaaacat caatatccaa ttcagtatta tccaatccag tatttgtacc atccaaagtt
     1561 tcttccaacc tttcctcttc caaccttttt tcttctaatt ttctttcttc caaccacttt
     1621 ttttcttcta aattttcttc ttcatccacc tcatgatcta ctacatccat taaataatct
     1681 gtttttgtag gtgactcatc atccatatta ttagcattta cattcacaat cggattttgt
     1741 tcaacacttt tattatcatc aaataaaaac gaccaatctg ttgccgctcc atgtactgga
     1801 gcaaaaatat cgtattcatt tcagtattga tccacaaaaa tataagttaa ttccattttt
     1861 ccaacaattt gtttttccat aggaataaat ataattccga tacaaaaaaa agcaacagca
     1921 acaactatga cagaaatcca ttttagtata gttttcattc tacttgaaat aatattataa
     1981 aacacatagt gattataatc agatttttta tttttgcaaa tcttttgaaa gaaaatctat
     2041 aaaaaacata aaatacattt accacacaca caaacaagaa atttataatt cattataaaa
     2101 tttaacaatt acaaaaaaac aaaggataat agcacttgtc ccattcgcgc ctataaaatt
     2161 acaggactgc gaaactgttt tataagttac gaactttatg aacttacatc aactggtcat
     2221 tgattttctt tatttttttg gttttttttc atttcttgaa tatctatttc caatcattct
     2281 gattgttttt tatccacctc ctccaaattt aatcacaatc tagcagaaat ttcagcatct
     2341 acaaactttc tttttctacc atactttttt gcagaatatt ctttaagaat tggtacaatt
     2401 tttttatttt tataatctca ggtatatata gaattcatac taaatggacg agtagttgag
     2461 ttatcaatat tcaatttaat ataagcttta tagtttgcta tacctgtaat atctcacatt
     2521 cacaaaacag gctgatattc tttttccaaa aactctgcat caggtgcacc tactttaaat
     2581 gacatcatag ttcccacatt accaaataca gccgctttta catctcattt tcatcattca
     2641 gatctattta atcactgtca tgcttccaat tgagctatat attgatgagc cataattaga
     2701 cataatctat actttctagc ttctgataat atgtcagcaa atgttccaga aacaaagttt
     2761 tggaattcat caacatacaa ataaaaatct tttctatctt tttccgggat tttcccacga
     2821 gccatagctg ctgtataaat tttagaaacc aaaatcatac ccaaaagctg agcattcatg
     2881 tctcatattt tacctttaga taaattaata agtaaaattt ttcattcatc cattacatca
     2941 tttaatctaa aagcggattt tgtctgtcaa attatatttc taataagtct gttcgtatta
     3001 aaagacacaa attttgaact aaaatatgga ataatttcgg atttttctct atctcacata
     3061 gcattataag ttttctccca gaagtttcta acaactgcat tttttacttt agctacttta
     3121 tattccctaa atgcctcatc agtataaagt ctaacaacat ccagtattgt tggtctatct
     3181 tcaaaatctt ccataattgt caaactacca tatttgaaat attcctggat acgaggacca
     3241 aaaatttctg gaccaaacat ttttataaaa atttcagtag catcatttac tatcctatct
     3301 gcttcatcaa tattatcaat ttcatataaa ttcaatcaca ttggtctttc ttcatctcaa
     3361 gcatcaaaat atatcacatc ttttgctctt tcttttggta tatactccaa catatcctct
     3421 accaaatctc catgtggatc aataacacaa accccatccc cattccaaat atcttgtctt
     3481 gctaaagttt gtaaaaaaac agacttacca gtacccgact tcccaataca atagtgatgt
     3541 ctagttctat cgtttctttt catatatact ggtgaatatg tgttcctata aatatttgtt
     3601 cataacaaaa ctcaatcttt ataaatttta tatcaataaa tttccttctc ttcttcaact
     3661 ttgacaaact tttttcaatc tttttcctct atttcttttc atgctaattc attttgttta
     3721 aaattttcga ttgggactag tttttcttct attacttttc ttgatcaaac agctcgatga
     3781 tctttatatt ctttcaatat ttcagacaat tttcatcatt tatatttttc agcaacaata
     3841 ccactcatta taaaaccact ttcttttcaa tcacgaaaag atggtaaatt agatggagca
     3901 ggtaaaattt tatattgcat tcgttctata atatttgaac gattatatgt ataatccggg
     3961 aaatgatata aggaagttaa ctcatttaat gtaaaagtat tagaattata aaacaaattt
     4021 gtcaaattat aagatgccgc tattctccat aaaggtcttg ctaaaaaacc aaatatagtt
     4081 gccctatttc ccaattgctc cagttcgttt caatactgat ccgtataaac attataggaa
     4141 ctaactaaat tctgtatatt gttttttaat ttatcttcat gatctgaaga ggtaactaat
     4201 attagtccag actcaaatgc aaccgttcat atttcttctg ccatgtcatt gattgtttct
     4261 tctttggctt ttaccatgcg caccattgaa atattttcct ctttctgttt aacaggaatt
     4321 tctcaatgaa acaataattt aaaaaatgtt tttattcaat accatattgc tgggaaaaaa
     4381 ccacttggcc tcaaatcaag atttttatac aatctatcca cctgtctttt tgctttttcg
     4441 ttccatttat cggaaagtgg cttaattgtc attataactg ttgctgtatc cgattttgat
     4501 atattattca tcgaatcaat aatattattt ataggatcat ccatcatcct tttataaagc
     4561 ctgatagtat aaatagaact tttttctgat tgtaaaggca taactttatg atatttcttc
     4621 ctaaaaatat taggcctttt tacgacttcc aaggcactat ctgcaaattg tgctcatatt
     4681 gctcattcta aaattttttg atattcaggg aaacatcata caataaaatt taaaattcat
     4741 cactcatagt gatacatcaa aactattcta ggttttctaa aaattctata cattatttta
     4801 tctttcatac taagtcttca aattttatga aaatttaaat aaacttgaga cattcttcat
     4861 atcttttctt tcatatcttt tgctaattct tttgcttgtt cacgatcaat tttactatct
     4921 catcttggga caagtacttt catgtaaacc attctatgat tgttc
//
LOCUS       DAOSID010000055         4953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_15174_length_4953_cov_9.768273, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000055 DAOSID010000000
VERSION     DAOSID010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15174_length_4953_cov_9.768273"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..540
                     /locus_tag="PK674_02765"
     CDS             139..540
                     /locus_tag="PK674_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15487.1"
                     /translation="MRKEKYIKIIGKHGNTIKIISTLLTILAMIVAARTYITYNSIIE
                     AINNVNHNIRAVEDEIAYSNNFLKYYLDSEYSDYFLAHKNNILFNGEFIIKFQAPLEN
                     EKEEKNIENKNIIQTPQESRQHFIKSKIHKK"
     gene            585..1070
                     /locus_tag="PK674_02770"
     CDS             585..1070
                     /locus_tag="PK674_02770"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOG15488.1"
                     /translation="MKKLLGLILILGATVLILSACGKRLEITNGDRVTLMYDSFSKDG
                     NIIEKNQKTILIVGLGQSFPAFEIELLGMKKGETKQFEATSENGYAIRHDNSKIQWIG
                     PEIFHSLNIEPKEGEAVSLGTMDGIVIEVDTNNIIVDFNPNYTREDVSFKVKVLEVER
                     E"
     gene            complement(1140..2168)
                     /locus_tag="PK674_02775"
     CDS             complement(1140..2168)
                     /locus_tag="PK674_02775"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="LD-carboxypeptidase"
                     /protein_id="HOG15489.1"
                     /translation="MILKIPKKLQRGSKVAIVSSSWGGPGTIPNRYNNGIKQLKEQFG
                     VEIIEMENAKKDPEFVYNNPKKRAEDLMNAFLDPNIDAIFSSIGGDDSIRILPYIDFD
                     IIKNNPKIYMGYSDSTITNFICYKAGIRSYYGPSIMAGFGENGGMFEYMIESVEKTLF
                     SNEIIGEIKPNTSGWTNEFLDWGNPKNLNIKRKLNPNTGWRFIQGGGIVEGELLGGCV
                     DVFYFMWGTEIWPDKDEWKGKILFIETSEENISEDNFERILRTMGIQGILENLNGIIV
                     GRAQNEKNYDNSLLKIINGEFGLYNLPIITNMDFGHTDPVFVLPIGAKMTLDFDNKKV
                     FINESGVL"
     gene            complement(2361..4178)
                     /locus_tag="PK674_02780"
     CDS             complement(2361..4178)
                     /locus_tag="PK674_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="restriction endonuclease"
                     /protein_id="HOG15490.1"
                     /translation="MQVTINYDGYRYRTELDWPFFGETKYLEGYSEEYIRNEIEKQVE
                     YRGKREKFVKDIYNQITRNFEFDDLFYRTFFEINKKKYYFCDYKITEMFNKIKDFVNE
                     EEKYFRIKLRPNESLAKELTDLSIKRLEEVDLILESNSEGFPNFKSQNDKGDINIIEY
                     FCLRNQLDEPKKGEISREPMFTDEKYNIGFMLFGKEKKKNEMKELYEQDYSERKKAKD
                     SVEKKYQTDLREYKKLKTEQEKSNEKIKELEIKIKDHRKNAEIGISEILSGYKNGDKL
                     QTERYMRGVLLNSSYPAFFPKQFELEYNQKNQILILDYFLPEIESFPTLNEIRYIKSK
                     EEIKEFHIKESKISQLFDFSMYATTLRVIKEVFDNDNNDKISAVSFNGWVNCINKTTG
                     KREDNCILSIQIKKDSFVDIELENVDPKSCFKSFKGIGSAKLSGITPVVPILQMNKTD
                     KRFSKAYEVVNKIDTATNLASMDREDFEHLVREIFEKEFSGNGGEVKITQASKDGGVD
                     AVAFDPDPIRGGKIVIQAKRYTNTVGVSAVRDLYGTVMNEGATKGILVTTSDYGADSY
                     EFAKGKPITLLNGANLLYLLEKHGHNAKIDIKEAKKLLE"
     gene            complement(4506..>4953)
                     /locus_tag="PK674_02785"
     CDS             complement(4506..>4953)
                     /locus_tag="PK674_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15491.1"
                     /translation="SAIDHRPLNFGELEMMLNRKKIDDKENYKDVLDDMSDTYREVIA
                     NSEKIAKARGENQLSLILQKQKKGSKTIFLSATPAQYELDLSDQVVEQIIRPTGLLDP
                     ITYIYPKSVSFEDLETSLDSLIKKKPHLEEFLDGYSIKNSAKEVFE"
BASE COUNT         1728 a          765 c          545 g         1915 t
ORIGIN      
        1 aataaaacat acaactatct tctagttttt ttacttgaat tttcaaccaa gaattataat
       61 aataattcaa cgagataagt taaattcttt ttaatgatca ttataaatta ataaacaatt
      121 attatataac attattcaat gcgaaaggaa aaatatataa agataatatg aaaacattga
      181 aatacaataa agataatttc aacactttta acaatattag ctatgatagt tgcagccaga
      241 acatacataa cctacaactc aataattgaa gcaatcaaca atgtaaatca taatatccgt
      301 gcagtagaag atgaaattgc atattcaaac aattttctca aatactacct agattctgag
      361 tattcagact atttcttagc acataaaaac aatatattat ttaattgaga attcattata
      421 aaatttcaag cccctttaga aaatgaaaaa gaagaaaaaa atattgaaaa taaaaatata
      481 attcaaaccc ctcaagagtc ccgacaacat tttataaaat ccaaaataca caaaaaataa
      541 actattctga agataagttt tttaaccttt aaataaaacc taaaatgaaa aaattactgg
      601 gtctgatttt aatactagga gcaacagttt tgattttaag tgcttgtggt aaaaggctag
      661 aaatcaccaa ctgagacaga gtaaccttga tgtacgactc tttttcaaaa gatggtaaca
      721 taatagaaaa aaaccaaaaa accatcttaa tagtatgact gggacaaagt tttccggctt
      781 ttgaaattga attattatga atgaaaaaat gagagacaaa acaatttgaa gcaacaagtg
      841 aaaattgata tgccattaga catgataata gtaagattca atggataggt ccagaaatat
      901 ttcatagtct aaatatagaa cccaaagagt gagaagctgt atctttgtga acaatggact
      961 gaatagtaat agaagtggat acaaacaaca taatcgtaga tttcaatcct aattatactc
     1021 gtgaagatgt atctttcaaa gtgaaagtcc tagaagtaga aagagaataa tatcaactta
     1081 tttttttgtg cattattaaa tattcaggac atgcaaaaaa attaaaatca attaaaaatt
     1141 tataagactc aagattcatt gataaatacc tttttattat caaaatctaa agtcattttt
     1201 gctcaaatcg gaagtacaaa aacaggatct gtgtgtccaa aatccatatt tgtaattatt
     1261 ggtaaattat ataatcagaa ctctcaattt attatcttta ataaactatt atcataattt
     1321 ttttcgtttt gagctcttcc tactattatt caatttaaat tttctaaaat tcattgaatt
     1381 cacatagtcc ttaatattct ttcaaaatta tcttcagata tattttcctc agaagtttca
     1441 ataaatagaa tttttcattt ccattcatct ttatctggcc aaatttcagt tcaccacata
     1501 aaataaaata catctacaca tcatcataat aattctcact ccactattca acctcattga
     1561 ataaatctcc atcatgtatt tggatttaat tttcttttta tattaagatt ttttggattt
     1621 caccagtcta aaaattcatt tgtccatcaa gaagtatttg gtttaatttc tcaaattatt
     1681 tcattactaa acaatgtttt ttctacactt tctatcatat actcaaacat tcatcaattt
     1741 tctccaaatc aagccattat agaaggtcaa taataacttc ttattcatgc tttataacaa
     1801 ataaaattag taattgtact atctgaatat cccatataaa tttttggatt atttttaata
     1861 atatcaaaat ctatataagg taatattcta atactatcgt ctcatcaaat acttgaaaat
     1921 attgcatcaa tattcggatc taaaaatgca ttcattaaat cttctgctct tttttttgga
     1981 ttattataaa caaattcagg atcttttttt gcattttcca tttcaattat ttcgactcaa
     2041 aattgttctt ttaattgttt aattcaatta ttatatctat taggaatagt tcaaggtcat
     2101 caccaagaac tactaactat tgcaactttt gatcaccttt gtaatttttt agggattttc
     2161 aaaatcataa ttaagtaatt attaataaaa ttattctaac gtttcttgcc ggtccgaagt
     2221 gcaaagtgaa atgtagtatt ttggacagca aatgttgtcg gagccgaatt gcatatgatt
     2281 ttatctctcg ttcactatat ttcaaacatc catttttata caatttttag aagggcataa
     2341 ctcccgaaat aaggaaaaaa ttactctaga agttttttcg cttccttaat atctattttt
     2401 gcattatgtc agtgtttttc tagcaagtaa agtagattag ctcaatttaa aagtgttata
     2461 ggtttccctt tcgcaaattc atatgaatcg gctcaataat cagatgttgt tacaagtatt
     2521 cattttgtag ctcactcgtt cattactgtt caatataaat ctctcacagc agatactcaa
     2581 acagtatttg tatatctttt tgcttgaata actatttttc atcatcttat tggatcagga
     2641 tcaaatgcaa ccgcatctac tcatcaatct ttacttgctt gtgttatttt aacttctcat
     2701 caatttccgc taaattcctt ttcaaatatt tcacgtacta aatgttcaaa gtcttctcga
     2761 tccatagatg ccaagtttgt tgctgtatca atcttattta caacctcata agcttttgag
     2821 aatcttttat ctgttttatt catttgaagt atgggaacaa ctggagttat tcaacttaat
     2881 tttgcacttc aaattcattt aaagctctta aaacatgatt tggggtcaac attttcaagc
     2941 tcaatgtcta caaatgagtc cttctttatt tgtatagaca aaatacaatt atcctccctt
     3001 tttcatgttg ttttattaat acagtttacc catcaattga aagaaacagc acttatttta
     3061 tcattattat cattgtcaaa cacttcttta attactctaa gtgttgttgc atacatagaa
     3121 aaatcaaata attgagatat tttactttct tttatatgaa attcttttat ctcttctttt
     3181 gactttatat atctaatttc atttaaagtt gggaaagatt ctatttctgg taaaaaataa
     3241 tccaatatta aaatttgatt cttttgatta tactcaagtt caaattgttt agggaaaaat
     3301 gctggatatg aagaatttaa taaaacacct ctcatatacc tttctgtttg taatttatct
     3361 caatttttat atcagcttaa tatttcggag attccaattt cagcattttt tctatgatct
     3421 ttaattttta tttctaattc ttttattttt tcatttgatt tttcttgctc tgtttttaat
     3481 tttttatatt ctcttaaatc agtttggtat tttttttcta cagaatcttt tgcttttttt
     3541 cgttcagaat aatcttgttc atataattct ttcatttcat tttttttctt ttcctttcaa
     3601 aataacataa atcctatgtt gtatttttca tctgtgaaca ttggttccct agatatctct
     3661 catttttttg gttcatctaa ctgatttctt aaacagaaat attcaattat attaatatct
     3721 catttgtcat tttgagactt aaaattagga aaaccttcac tgttgctttc aagaatcaaa
     3781 tcaacttctt ctaacctttt gattgataag tcagttaatt cttttgccaa tgattcattt
     3841 ggtctaagtt taatacgaaa atatttttct tcttcgttta caaaatcctt aattttatta
     3901 aacatttctg ttattttata atcgcagaaa taatatttct tcttatttat ttcaaaaaat
     3961 gttctataaa aaagatcatc aaattcaaaa tttctggtta tttgattgta aatatctttt
     4021 acaaactttt ctctttttcc tcgatattca acttgttttt ctatctcatt ccttatatat
     4081 tcttcggaat atcactctaa atattttgtt tctcaaaaga aaggccaatc taattctgtt
     4141 ctataacgat atcaatcata attgatagta acctgcatta tttagtatgt aagaaataaa
     4201 attgctcctt agaacaaaac tctctcccaa aaatcgtcca ctgtattccc aaactttgat
     4261 tttcgagata agagacaaaa tcatatgtca tctaactcgt cgctatgcaa agtgttttgc
     4321 ttgatacaaa aatttataga ttatgcaaaa tatgtcggat aaagccacaa taatgaattt
     4381 tataagtaat acatatgaaa attcaagaaa ttatttgtct cttttgtttc tagaaaaaaa
     4441 gtgatagatt ttatttttac aatccaaaga taggaatcca aagactatta atcttcataa
     4501 agaaattact caaacacctc tttagctgaa tttttgattg aatatcaatc taagaactct
     4561 tctaaatgag gttttttctt tattaatgaa tctaaactag tttccaaatc ttcaaaagaa
     4621 actgacttag gataaatata tgtgatggga tccaacaatc ctgttggcct aataatttgt
     4681 tctacaactt gatctgataa atctaattca tactgagctg gtgtagcaga aagaaaaata
     4741 gtttttgagc ctttcttttg tttttgcaaa atcaaggaaa gctgattttc tcaacgagct
     4801 tttgctatct tttccgaatt agctattact tctctgtagg tatcagacat atcatctaaa
     4861 acatctttat agttttcttt atcatctatt ttttttcgat ttaacatcat ctcaagctct
     4921 caaaaattta acggacgatg atctatggca ctt
//
LOCUS       DAOSID010000056         4874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_15481_length_4874_cov_13.288442, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000056 DAOSID010000000
VERSION     DAOSID010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15481_length_4874_cov_13.288442"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..>1186)
                     /gene="sufB"
                     /locus_tag="PK674_02790"
     CDS             complement(3..>1186)
                     /gene="sufB"
                     /locus_tag="PK674_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=25
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOG15492.1"
                     /translation="DGLVWYARPSSDNTGYATNRDDVPQEIKDKFDKLGIPEAEKKYL
                     AGAGGQMDSVNFYHKIKEKWAKVGVIFEDMPTAIQTHPELVKKYFMKLVPPTDHKFAA
                     LHGAVRSGGTFIYVPKGVKVDEPLQAYFRMNTYAGGQFEHTLIIIDDDAECNYIEGCS
                     APKYDKSSIHTGLVEIFVGKNSKMKYASVENWSTNTYNLNTKRALVEENSYMERVSGN
                     LGSQTTMLYPCSILKGDNSKTEMFGIVLASKGQNQDIGSKVIHIGKNTSSNIVSKSIS
                     KDGGISTYRGLVSISKSADNAVSNTQCDAFILDKDSVSITIPTIKDNNGNASISHEAS
                     TGKIDESKLFYLSSRGIEISKATSMIVNGFFSQVSKKLPLEYAGELNTLIDIEMENSV
                     G"
     assembly_gap    1187..1286
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1458..2177)
                     /gene="sufC"
                     /locus_tag="PK674_02795"
     CDS             complement(1458..2177)
                     /gene="sufC"
                     /locus_tag="PK674_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOG15493.1"
                     /translation="MMFEINDLSVSVGEKKILKNINLKFELGKNYCILGKNGSGKSTL
                     ALTIMGHPNYKVDTGSLNIEGLDVLEMSPDKRAKLGIFLAFQQIPEIEGVKLFEFLRN
                     IYNTHTGENTTFLKFKDIIIPLIQEVGLDKEFLRRDLNVGFSGGEKRKIEILQIKLLK
                     PKYIFLDEIDSGLDVDAFKSITKMIGELNNQENCFVFITHHFEIIQNIDIDEVIILNN
                     GEIKDRGDKKLIEKIKKEGFK"
     gene            2722..3414
                     /locus_tag="PK674_02800"
     CDS             2722..3414
                     /locus_tag="PK674_02800"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DNA alkylation repair protein"
                     /protein_id="HOG15494.1"
                     /translation="MYKHIENELLKLKNPDKAKILQGFFKTGKGEYGEGDVFLGITVP
                     QQRNLVKKYWANIDLEDIQKLLENKYHEFRMTGLLFLVQKYEKSNITRKDEIFRFYID
                     NIKHINNRDLVDLSCHKIIGNFLLTRDRGILYKFAESSNMRIQRIAIVSTYTFIKNGD
                     FADTIKISKLLLDHKHDLIHKAVGRMLREIGKKDEDILIEFIEENASKMQRTTLRYAI
                     EKFDTNKRKHFL"
     gene            complement(3473..4063)
                     /locus_tag="PK674_02805"
     CDS             complement(3473..4063)
                     /locus_tag="PK674_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15495.1"
                     /translation="MAKDLISSKKLMKLLHKFNRYNKHEKVFVGYLFILLFLLLFFPI
                     LKVTSLRGTGGYGIWIRKPEMFKTMLIVLVSMLILLGWNLSFKFKNLFLSYFGAREND
                     SLINFLFLFIITTSFFAITDTIHVSSGVTSRVIVSGAGKFIQIRLLIGLIFSLISVVK
                     MAQETGKKTKIINMVDDEHKKTELIKDDIKKGLFDR"
     gene            complement(4134..4559)
                     /locus_tag="PK674_02810"
     CDS             complement(4134..4559)
                     /locus_tag="PK674_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15496.1"
                     /translation="MNVGANSNSVQDLILIILAVVIIIIAVGCIYAFIRAIFLFIFSQ
                     GKEDKIKKAWNSIRYMIMGIFLSVFLLFMFPLIFKRVGLDNHEEYTAKNIFNQAGTII
                     NKTFQLKDIVRESQKKNQYDGNLYYEDNFSERYVEDYSL"
     gene            complement(4591..>4874)
                     /locus_tag="PK674_02815"
     CDS             complement(4591..>4874)
                     /locus_tag="PK674_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15497.1"
                     /translation="EEKKSEEVQEEEKSEEVQEEEKSEEIQEEEKSEEVQEEEKKSEE
                     VQEEEKSEEVQEEKKSEEVQEEKKSEEVKDGEGVEEKVGETLSKSELSE"
BASE COUNT         1705 a          805 c          503 g         1761 t
ORIGIN      
        1 aattatcaaa cactattttc catttcaata tcaattaaag tattcaattc tcacgcatat
       61 tccaatggaa gttttttact tacttgagaa aaaaatcagt ttactatcat gctagtcgct
      121 ttacttattt caataccacg agagctaaga taaaataatt ttgattcatc aatttttcat
      181 gtacttgctt catgtgaaat acttgcattc ccattattat ctttaattgt tggtatagta
      241 atgctaacag aatccttatc caaaataaaa gcatcacatt gagtgttact aacggcattg
      301 tcagcacttt tggatataga aacaagacct ctatatgtag aaattcctca atctttagaa
      361 atagatttag aaacaatgtt ggaagaggta tttttcccaa tatgaatgac tttcgatcaa
      421 atgtcttgat tttgtccctt ggaagcaaga actattccaa acatctctgt tttactatta
      481 tctcatttca aaatactaca tggataaagc attgttgtct gagatcacaa gtttcagcta
      541 actcgttcca tatatgaatt ttcttctact aaagctcttt tcgtgttcaa gttatatgta
      601 ttagttgacc agttttctac cgaagcatac ttcatttttg aattttttcc cacaaaaatt
      661 tcaaccaaac ctgtatgtat cgaagactta tcatattttg gtgctgaaca tcattctata
      721 taattacatt cagcatcatc atctataata atcaatgtgt gttcaaactg tcatcacgca
      781 taagtattca tcctaaaata tgcctgcaaa ggttcgtcta cttttactca tttgggcaca
      841 tatataaaag ttcatcaact tcgtacagct caatgcaatg ctgcgaattt atgatctgta
      901 ggtggaacta atttcataaa atattttttt actaattctg gatgagtttg aattgcagtt
      961 ggcatatctt caaagataac tcaaactttt gcccattttt ctttgatttt atgataaaag
     1021 tttacactat ccatctgtca tcatgctcca gccagatatt ttttttctgc ttcaggaatt
     1081 cataacttat caaacttgtc tttgatttct tgtggcacat catctcgatt tgtggcatat
     1141 ccagtattat cagaacttgg tcttgcatac caaactaatc agtcatnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnntcca atgattttaa tctgagttcc aacatccatt
     1321 caggttcttt ttgatcagca gaaatttttc tcacagtatc ttctgtcagt catgttagat
     1381 aaacatcata ttttatatta tttggtgttt ccaaagcttt ttttaaactc atgatcaaaa
     1441 ttattgtaaa gccacattta cttaaatcat tcttttttta tcttttctat tagtttctta
     1501 tctcatctat ctttaatctc tcaattattc aatattatca cctcatctat atctatgttt
     1561 tgaattatct caaaatgatg agtaataaat acaaaacaat tttcttgatt attcaactcc
     1621 cctatcatct tggtaataga tttgaaagca tcaacatcca atcaactatc tatttcatcc
     1681 aaaaaaatat attttggttt gagtaattta atttgtaata tttcaatttt tctcttttct
     1741 catcaactaa aaccaacatt caaatccctt cgtaaaaatt ctttatccaa tcatacctct
     1801 tgaattaacg gaatgataat gtctttaaat ttcaaaaacg tggtattctc tcaagtatga
     1861 gtattgtaaa tatttcttaa aaactcaaaa agctttactc cttctatttc tggaatttgt
     1921 tgaaaagcta ggaatattca aagttttgcc cttttatcag gcgacatttc caaaacatct
     1981 aaaccttcaa tattcaaact tcaagtatct actttatagt ttggatgtca cataatcgtc
     2041 agagccaaag tagattttcc acttcaattt ttacccagaa tacagtagtt ctttcacagc
     2101 tcaaatttaa gatttatatt ttttaatatc tttttttctc caacacttac agataaatca
     2161 tttatttcaa acatcatttt ttcttatata aaatacaata ttttattata tacaaaagct
     2221 agtaaaaaaa tcaaactaaa tcccaaaaat atttatcaaa acaaacccat tacacaaata
     2281 aatgtaactt cacaacataa caaaagactt gatattaagt aaggtttcct tataccgctt
     2341 aaaacaaaaa taaaataaca attaaaaatt ataattatgg aagaagaagt ttacaccctt
     2401 attattgcga cgatattgta cattttccta ttcttattga gtattaaatt caactataat
     2461 aggaaaatag aaattgtttt atttacaata gcttacgttt ttctaatttt ttcaataatc
     2521 tatcctcttt ttctttctgg atggtataaa tgtttatgtt tagtaggatt catattttct
     2581 tggttctact gtataaatga aaaaacaata gaaaacattt taagttaaaa ataatgtaaa
     2641 atcggattaa aatccagctt tatttaattg taaaaaacaa atatttctgt ataaaaaaat
     2701 tatttaacta tacataatac tatgtacaaa catattgaaa acgaacttct aaaactaaag
     2761 aatccagata aggctaaaat cctacaatga tttttcaaaa cttgaaaatg agaatatgga
     2821 gagtgagatg tttttttatg aattactgtt ccacaacaaa gaaatttagt aaaaaaatat
     2881 tgggcaaata tagatttaga agatattcaa aaactactag aaaacaaata tcacgaattt
     2941 cgtatgactt gactattatt tctagtccaa aaatatgaaa aatcaaatat tacaaggaaa
     3001 gatgaaattt ttcgcttcta tatagataat atcaaacaca taaacaatcg agatttagta
     3061 gatttatcat gtcacaaaat tatatgaaat tttttactaa cgagagatag aggtatttta
     3121 tataaatttg cagaaagtag caatatgcga atacaaagga ttgctatcgt tagtacttat
     3181 acttttataa aaaactgaga cttcgcagat actataaaaa tttctaaact acttttagat
     3241 cacaaacacg atttaattca taaggcggtt tgacggatgc ttagagagat cggcaaaaaa
     3301 gatgaagata ttttgataga atttatagaa gaaaacgcat ccaagatgca aagaactact
     3361 cttagatatg ctattgaaaa atttgataca aataaaagaa aacatttttt ataaaaaaag
     3421 ctatctaaac acttgcccag atagcttttt tatgataaaa atattttgaa cattatctat
     3481 caaacaatca ttttttgata tcatctttga tgagttcagt ctttttgtgt tcatcatcta
     3541 ccatattgat aattttggtt tttttacctg tctcttgcgc catttttact acagatataa
     3601 gactaaatat caatcatatc aataatcgta tttgtataaa ttttccagca ccagatacta
     3661 ttactctgga agtaactcaa gaagaaacat ggatagtatc agttatggca aaaaaagatg
     3721 ttgtaattat aaataagaat aagaaattaa ttaaggaatc attttctcta gctccgaaat
     3781 atgataaaaa caaattttta aacttaaaac ttaaattcca tcataataaa ataagcattg
     3841 acaccaatac tatcaacatc gttttaaaca tttctggttt tcgtatccat ataccatatc
     3901 caccagttca tcttaaagaa gtaactttta aaatagggaa aaataataac aaaaacaaca
     3961 atataaataa gtatcataca aaaacctttt catgtttatt atacctatta aatttatgaa
     4021 gtaatttcat caacttttta gatgatatca agtcttttgc cataatattt taataaaaat
     4081 ataaatttac attcttactt taactttcat agaaagtttt tcaatgaaaa aaattataag
     4141 ctataatcct ctacatatct ctctgaaaaa ttatcttcgt aataaaggtt accatcatat
     4201 tgattctttt tttgactttc tcttacaata tctttcaatt gaaaagtttt atttattatt
     4261 gttcatgctt ggttaaaaat atttttcgca gtatattctt catgattatc caatcctact
     4321 cgtttaaata ttaatgggaa cataaataac aaaaatactg ataaaaatat tcccataatc
     4381 atatatctaa tactattcca agccttttta attttatctt cttttcattg agaaaaaata
     4441 aataaaaaaa tagcacgaat aaaagcatat atacatcata ccgcaattat aataataaca
     4501 acagccaaaa tgatcaatat taaatcttga acagaatttg aattagcacc tacattcatc
     4561 aaaactaaaa ttaaaaatta aaaaactcac ttattctgac aattccgatt ttgaaagtgt
     4621 ttctccaact ttttcttcaa caccctctca atcttttacc tcttcagatt tcttttcttc
     4681 ctgaacttct tcagatttct tttcttcctg aacctcttca gacttctcct cctcttgaac
     4741 ctcttcagac ttcttttctt cctcctgaac ctcttcagac ttctcctcct cttgaatctc
     4801 ttcagacttt tcctcctcct gaacctcttc agacttctcc tcctcttgaa cctcttcaga
     4861 cttcttttct tcct
//
LOCUS       DAOSID010000057         4840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_15610_length_4840_cov_15.112017, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000057 DAOSID010000000
VERSION     DAOSID010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15610_length_4840_cov_15.112017"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PK674_02820"
     CDS             <1..395
                     /locus_tag="PK674_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15498.1"
                     /translation="LIDKINATCDALISALEINSEKVSSYNMTIVDRMNVNSEFIDLV
                     VKNIISIKTHLGELKDGLNNQKLLSDTLDLENLISGLQNTSSKLNCLLISDNNFNNIY
                     SMAILEIQDLIQKVLDEIKIGEIVLKGV"
     gene            complement(532..2955)
                     /locus_tag="PK674_02825"
     CDS             complement(532..2955)
                     /locus_tag="PK674_02825"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="valine--tRNA ligase"
                     /protein_id="HOG15499.1"
                     /translation="MSKYNPKESEKKRQDFREENKIYTFDLNSDKEVFSIDTPPPTVS
                     GKLHIGHISSYTQAEIIARYKRMKGYNVYYPMGFDNNGIPTELLVEKDLKINIKEMER
                     KDFIQKCLEVNQKYVKIYESLRKTMGLSIDWTKIYSTIDPKTQQIVQKEFVKLYKQGH
                     IVAKEFPALRCTKLQATIAQAETEDQEFEEYFNDIEFTLEDGTKLTIATTRPELLPAC
                     KAVFAHPDDTRYQKYFNKKITTPLGDTVPLLPDDKAKIDKGTGLVMCCSYGDETDVYW
                     FQKHGLEAKICIDRYGKMQNTGLTEINGLKVNEARDKIMDILEQKGVVKNRTKIVQSK
                     SISERGKVPVEIIPVHQRFVNILDKKDILLEQNDKMRWFPEFMKKRSNDWIENLHRDW
                     NISRNRKFGIPIPVWYDINSGDVILPSMEQLEKGPVDPSVDLPDGYTKEQVRGETLVL
                     DTWFTSGLSPLINKNLLDKNGFDTKDFFPMSMRPQSHDIIRTWLLYTTLHSYLRDGKI
                     PFQNIMISGFVMAEKGQKFSKSKGNAFDPEDLINQRGADAIRYWTAGSQLGKDILFEE
                     NEIKNGQKLITKLWNASNFVQMLTQGFDPKQKFDTNKLLATDQRIIARTNETIEKMTK
                     YLDNYEYGLAKIAFEEFFWADFCDNYLELVKLRLYKPELFENGEEKKLAGQWTLYHIL
                     FDTLKLIAPYLPHITEEIYQNYFKQYENIISIHKTEFPTQILEINNQAEQIKEFEKIK
                     EIIESVRKFKTENQISMGAELKSLDIHGSKEFLEIARKYLDDIIGVTKAQKVDLIESH
                     EENFICSKL"
     gene            complement(3185..4606)
                     /gene="dnaX"
                     /locus_tag="PK674_02830"
     CDS             complement(3185..4606)
                     /gene="dnaX"
                     /locus_tag="PK674_02830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOG15500.1"
                     /translation="MNFILSKNIMSLANKYRPNTFDKIIGQDHITHILKAQMKNEQRN
                     NHNYLLFGPRGTGKTTAARILSKAINCLDLQDGNPCNQCANCNTINKGQTFDYVEIDA
                     ASYTGVDNIREEILDKVPYPPTQLKKKIYVIDEVHMLSKSAFNALLKTIEEPNSAVGF
                     IFATTEIHKVPETIVSRCQVFNFKKILKETMTPHLQNICNLEGLSFEDEALNLISDIS
                     EGCVRDAVKYVDQISVFGSISMENVSNFLGIAGEAMIKDFLELIKSKDREAIFKKVDE
                     IHDQGIDLLQFAKQNIMYIDKNIMSDIDFFISVSEIFSEIIATAKYYPYPTILYKIAI
                     NKFLGQNNQVVNYQEPVKQENKIKENKTEESVVTETPISEEKNQNEAPGVESKEKEIL
                     NQLIEKVESKTLKDSLRDHIFIKNISDGKAYFVVINKIAEIALNRDDTKSNLEEMISE
                     IVGENITIDIQFQSKEDYFSSIL"
BASE COUNT         1362 a          716 c          726 g         2036 t
ORIGIN      
        1 ttttgattga taaaattaat gctacttgtg atgctttgat ttctgctttg gaaattaatt
       61 ctgaaaaagt ttctagctac aatatgacta ttgtggatag gatgaatgtt aattcagaat
      121 ttattgattt ggttgtaaaa aatattattt ctataaaaac tcatcttgga gaattgaaag
      181 atggcttaaa caaccaaaaa ttgctttctg ataccttaga tttagaaaat ctaatttctt
      241 gattacagaa tacatctagt aaattaaatt gtttattgat tagtgataat aattttaata
      301 atatctatag tatggctatc cttgaaatac aagatttaat ccaaaaggtt ttagatgaga
      361 ttaaaatctg agaaattgtg ttgaagtgag tttaatgagt tgaaagttcg tcaagttaga
      421 aagttcatca agttagaaag ttgtattctc ctaccacctg ggtgctgttc gcggagctaa
      481 ccaatcgcac ccggatggta ggaatgtagc tttcaacttg taactttcaa cttataactt
      541 actgcatata aaattttctt catgactttc tattaaatct actttttgag cttttgttac
      601 tcaaataata tcgtctaagt attttcttgc tatttccaaa aattcttttg atcaatgtat
      661 atctaaactt ttcaattctg ctcacattga aatttgattt tctgttttga attttcttac
      721 agactctatt atttctttga ttttttcaaa ctcttttatt tgttctgcct gattatttat
      781 ttccaaaatt tgtgtaggga attcggtttt atggattgat ataatgtttt catactgttt
      841 gaaatagttt tggtatattt cttctgtaat atgtggtaaa tatggagcta ttagtttcaa
      901 agtatcaaat agaatgtgat ataaggtcca ttgtcatgct aacttttttt cttcaccgtt
      961 ttcaaataac tctggtttgt acagcctaag ttttactaat tccaagtagt tatcacaaaa
     1021 atctgcccag aaaaattctt caaaagctat ttttgccaga ccatattcat agttgtctaa
     1081 atattttgtc attttttcta tagtttcgtt tgttctagct ataattcgtt gatctgtagc
     1141 tagtagttta ttggtatcaa atttttgttt tggatcaaat cattgagtaa gcatttgtac
     1201 aaaatttgaa gcattccaaa gctttgtgat gagtttttgt ccgtttttga tttcgttttc
     1261 ttcaaataat atgtcttttc atagttgact tcatgctgtc cagtatctaa ttgcatcagc
     1321 tcatcgttga ttgattaaat cttctggatc aaaagcattt catttggatt tactaaattt
     1381 ttgtcatttt tctgccataa caaatccaga aatcataata ttttgaaatg gtatttttca
     1441 gtctcttaga taggagtgta gtgtggtata caaaagccaa gttctgataa tatcgtgaga
     1501 ctgtggtctc atgctcattg gaaaaaaatc ttttgtatca aatcaatttt tgtctaaaag
     1561 gtttttgttt atcaaaggag aaagtcctga tgtaaaccaa gtatctaata ctagagtttc
     1621 tcatctaact tgttcttttg tatatccatc tggtaaatct acagacggat ctactggtca
     1681 cttttcaagc tgttccatcg atggtaatat tacatctcaa ctatttatat cataccatac
     1741 aggaatagga ataccaaatt ttctgtttct agaaatgttc caatctcgat gtaggttttc
     1801 tatccaatcg ttggatcttt ttttcataaa ttctggaaac catctcattt tatcgttttg
     1861 ttccaataat atatcttttt tgtctagaat gtttacaaat cgctgatgaa ctggaattat
     1921 ttctactggt acttttcatc tttctgaaat tgatttggat tgaactattt ttgtcctgtt
     1981 ttttactact cacttttgtt ccaaaatatc cattattttg tctctagctt catttacttt
     2041 tagtccattt atttctgtga gtccagtatt ttgcatttta ccatatctat ctatgcagat
     2101 ttttgcctct aatcagtgtt tttgaaacca atatacatct gtttcgtctc cgtaactaca
     2161 acacattaca agtcatgttc ctttgtcgat ttttgcctta tcgtctggta gtaaaggtac
     2221 agtatctcac aatggagtag taattttttt attaaaatat ttttgatatc tagtatcatc
     2281 tggatgtgca aaaacagcct tacatgctgg tagtaattct ggcctagtag tagcaatagt
     2341 caattttgtc ccatcttcta gagtaaactc tatatcattg aagtattctt caaattcttg
     2401 atcttctgtc tctgcttgag ctattgtagc ttgtagtttt gtacatcgta aagcagggaa
     2461 ctctttggca actatatgtc attgtttgta taattttaca aattcttttt gtactatttg
     2521 ttgtgttttt gggtctatgg tactataaat ttttgtccaa tcgatagata atcccattgt
     2581 tttccgtaaa ctttcatata ttttaacata tttttgattt acttccaaac acttttgaat
     2641 gaaatctttt ctttccattt ctttgatgtt tatttttagg tctttttcta ctaataattc
     2701 ggtaggaatc ccattgttgt caaaacccat agggtaataa acattgtatc atttcatcct
     2761 tttgtatcta gcaataattt ctgcttgagt gtatgatgaa atatgtcaga tatgtagttt
     2821 cccgctgact gtaggtggag gagtatctat actaaaaact tccttgtctg aatttagatc
     2881 gaaagtgtaa attttatttt cctctcgaaa atcttgtcgt tttttttctg attcttttgg
     2941 attgtacttg ctcataattt tttgaatagt taaatagtgg acttattagt aaatattgtt
     3001 tgattttttt caagatcaag cttatagagt tctcttccct tgttaaaggg agtctctttt
     3061 ccctccattg cttgtagctc tgcagaattg cggggctaca ttgtttttgt ttcatcattt
     3121 taacatttca acattttatt gcatacaaag acttcgcttg tggtggagac aggtattcat
     3181 cattttatag tatacttgaa aagtaatctt ctttggattg aaattggatg tctatggtga
     3241 tgttttctca tactatttcg gaaatcattt cttctaaatt actttttgta tcatctctat
     3301 tcaaagcaat ttctgctatt ttatttatta ctacaaagta agcttttcag tctgaaatgt
     3361 tttttataaa tatgtggtct ctcaaactat cttttaaggt tttgctttct actttttcaa
     3421 ttagctgatt caaaatttct ttctctttgc tttctacgcc tggtgcttcg ttttgatttt
     3481 tttcttctga aattggagtt tctgtgacga cgctttcttc tgttttgttt tctttgattt
     3541 tgttttcttg ttttactggt tcctgatagt tgacgacctg attgttttgt cataaaaatt
     3601 tatttatcgc aattttgtac aagatagtag ggtatggata atattttgct gttgctatta
     3661 tttctgaaaa aatttctgat acagagataa aaaagtcgat atctgacatt atatttttgt
     3721 ctatgtacat aatgttttgt tttgcaaatt gcaataagtc tattcattgg tcgtgaattt
     3781 catctacttt tttgaaaata gcttctctat cttttgactt tattaattcc aaaaaatctt
     3841 tgatcatggc ttcaccagct attcccaaaa aattgctaac attttccata ctaatgcttc
     3901 caaacacgct tatttggtct acatacttta cggcatctct tacacatcat tcagatatgt
     3961 ctgaaattaa atttaatgcc tcatcttcaa aagatagtca ctctaaatta caaatgtttt
     4021 gtaaatgagg agtcattgtt tcttttaaaa tttttttgaa attaaacact tggcaacgag
     4081 aaactattgt ttctgggact ttgtgtattt ctgtagtggc aaaaataaat caaacagcac
     4141 tatttggctc ttctatggtt tttaaaagag cattgaatgc agacttggag agcatatgta
     4201 cctcatctat tacatatatt ttctttttta gttgagtagg aggatatggt actttgtcta
     4261 aaatctcttc tctaatattg tctactcctg tgtagctggc cgcatctatt tctacataat
     4321 caaaagtttg tcatttgttt attgtgttac aatttgcaca ttgattacaa ggatttccat
     4381 cttgtagatc caaacagttt atggctttgg acaaaattct agctgctgta gtttttccag
     4441 tacctctagg tccaaaaagc aaatagttgt ggttgttcct ttgctcattt ttcatttgag
     4501 ctttgagtat gtgtgtaata tgatcttgtc atataatttt atcaaatgta ttcggacgat
     4561 atttgttggc taaagacata atattttttg acaatataaa attcattcat tttttataat
     4621 ataagattat attatttgtt ttcaattgga tttttgaaat ttttgtttat gaaaaatttc
     4681 caattcacta ttgtgctttg ttgtcaaata ttttttgaca ccagttaaaa ttacaataaa
     4741 gtattgaatt tgtgaaaaat tttgttataa gaaattttga aatgaatgag ggataattaa
     4801 caactaataa ttttgttcct tattaatggg aattgggaga
//
LOCUS       DAOSID010000058         4717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_16109_length_4717_cov_14.379665, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000058 DAOSID010000000
VERSION     DAOSID010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16109_length_4717_cov_14.379665"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..1136)
                     /locus_tag="PK674_02835"
     CDS             complement(222..1136)
                     /locus_tag="PK674_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15501.1"
                     /translation="MATKTKKSNRGRPRKDFSNMKIKKEESNIKRPRGRPRKEKKLEE
                     TVNTKISKHSTDINKLSKANEKIQLEISNANFSSRKVGENKKSNNFALGLLIFSMLLF
                     IFSLYKTFYSSSIQSNIISDGIVYKQINENTKSDEQELETKIVEYEVEKKDEAEKKDL
                     ENKKPADETEEDAHEFEIINTFYNTLNNKEFSELSKLVDSYLKSSNVYRTYYSNNWLN
                     KFTERLANKKVYLLNIKEINLENKKEGVKYYSYILRYKLNDSNELFTEEREASVITRG
                     NKKLIGSLHCITVGCSKMPFFNLPRYEK"
     gene            complement(1161..2180)
                     /locus_tag="PK674_02840"
     CDS             complement(1161..2180)
                     /locus_tag="PK674_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15502.1"
                     /translation="MKKLLTLILSLLGVIFFVGFSNVFATDCHTINYGGGNTACLSIN
                     KDGNVYTMSNGVNCPGGGCSLACTILLPNSELKEIGACNGSFTWNGASQGLVKIYTQF
                     NGNRKIIERNYNFSAGTWGSSTVTSPTLNKFQLTTNKSNPSTNQWVDLVVKARDSNNN
                     ILTNYTNKIKFDVYYRSKTSASSSWTKTTSSTYFTINSSYAKGYQFKTYDYGQKSFYD
                     FIKFHKNNLDYKIRVYDEDNPSIYKEIIFYISIFSSDIAGFSVKEVEMIQRIYTLRPQ
                     LIRQLKVEHPRFNTNTVWKNLGNDLYSNMKDVLDNKVGRKFTNYNKFFSDFMVWYKRT
                     LDVIH"
     gene            2304..2828
                     /locus_tag="PK674_02845"
     CDS             2304..2828
                     /locus_tag="PK674_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15503.1"
                     /translation="MKIVSKIITWILSIIFLFIIINLAIGVVKYEGLANYSDFLNQKE
                     RNTVVSQISIKDPISIFSLFYGEYTPREKNIDVRQENLEDKIIDQELQKDSQVDEEAK
                     LNVYDPDFEDDFNAFFGKTHISKKKSDLINEEGGAFVVPETSEQDNLNQTNSKQDNAN
                     TKTVGQQLLEKFSK"
     gene            2837..3481
                     /locus_tag="PK674_02850"
     CDS             2837..3481
                     /locus_tag="PK674_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOG15504.1"
                     /translation="MLQTINNQEDTVLSGSKNLIQKSFSENTKNTIRLVANTDFHYLL
                     LAENSESKIIFETKGENIKGNIYAIYFGSKPIKSDIQVNISSSNVDINVFMLSLLVSD
                     QEFNTNGNIYLLKDIHNSQGHLLQKNLILGEKIKVSSIPRLDVYSNDIKASHGFSIDR
                     LNQDKMFYLNSKGLSESTSKELIVHGNIKDILNKFTNLSEEQKNAIEDLILSKT"
     gene            3566..3913
                     /locus_tag="PK674_02855"
     CDS             3566..3913
                     /locus_tag="PK674_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15505.1"
                     /translation="MEKYFNLLLDLLEIKQNQNYSKILKIIQKNKDKKIFFYHQYSFI
                     FEGYQKLLNQELALEEFLQIGDINSKLKIDSKKIDSISFLQNKLKIDPNSIYIFDSIA
                     QGFLSEQANIFYC"
     gene            complement(3986..4453)
                     /locus_tag="PK674_02860"
     CDS             complement(3986..4453)
                     /locus_tag="PK674_02860"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOG15506.1"
                     /translation="MGYIFSAQTVLGKISIVEQDKKITHLFWDPKNIVNTYEYKETPL
                     LRDAFLQLEKYLEGKIKEFSLPLNPQGTDFMKSVWKALQNIPYGKTKSYKEIAETIGI
                     PKAMRAVGMANNKNPIAIFIPCHRVIGHNGDLVGYGGGLENKKKLLKLEGVEI"
     gene            complement(4457..>4717)
                     /locus_tag="PK674_02865"
     CDS             complement(4457..>4717)
                     /locus_tag="PK674_02865"
                     /inference="COORDINATES: protein motif:HMM:NF015442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="ATP cone domain-containing protein"
                     /protein_id="HOG15507.1"
                     /translation="MYDRQKLKKSLMLSFAKREFNNEKIENLLNNLENKRISEGTEIS
                     SKQIGDDILEMLKEIDPVVYIRFASVYKSFDSLEDFRNFIDD"
BASE COUNT         1661 a          707 c          583 g         1766 t
ORIGIN      
        1 ttttacttca tagtactata tcctgaaagt tcatggagtt tgaaagttta taaagttcgt
       61 aaagttcatg gagtttgaaa gttcataaag ttcataaagt ttgaaagttt ataaagtttt
      121 taaagtatta ataactttat aacttgcaac tttgctgttc gcggagctaa ccaattgcac
      181 ccgggtggta ggaatgtagc ttgtaacttg atagactagg tctatttctc gtatcttggt
      241 aggttaaaaa atggcatttt actacatcaa acagtaatac aatgcaaact tcaaattaat
      301 tttttatttc atctggttat tacactagct tctcgctcct ctgtaaatag ttcattacta
      361 tcgttcaact tataccttaa tatataagaa taatacttta ctccttcttt tttgttctcc
      421 aagttaattt ctttaatatt tagcaaataa acctttttat tggccaatct ttcagtaaat
      481 ttgtttagcc agttattgct gtagtaagtt ctgtaaacat tagaagattt caaatagctg
      541 tctacaagtt tgctcaactc agaaaactcc ttattgttta gtgtgttata aaatgtattt
      601 ataatttcaa attcatgagc gtcttcctct gtttcatcag ctggtttttt attttccaaa
      661 tccttttttt ctgcttcgtc ctttttttct acttcatatt caacaatttt cgtttccaat
      721 tcttgctcat ctgattttgt gttttcatta atttgtttat acactattcc atctgagata
      781 atatttgatt gtatactact gctataaaag gttttgtata aagaaaaaat aaacaataac
      841 attgaaaaaa taagtagtcc caaagcaaaa ttatttgatt ttttgttttc tcatactttt
      901 ctactagaaa aatttgcatt acttatttcc aattgtattt tttcgtttgc tttggaaagt
      961 ttattgatat ctgtcgaatg tttagaaatt tttgtattca cagtttcttc aagttttttt
     1021 tcttttcttg gtctaccacg aggccttttt atattacttt cttctttctt aattttcata
     1081 ttagaaaaat cttttcttgg tctaccacga ttggatttct ttgtttttgt agccattttt
     1141 gataaattaa ttatttaaaa ttaatgtata acatccaaag tacgcttata ccaaaccata
     1201 aaatcactaa aaaatttatt ataattggta aattttcttc aaaccttgtt gtccaataca
     1261 tctttcatat tggagtaaag atcgtttcat agatttttcc atacagtatt ggtgttgaat
     1321 cttggatgtt ccacttttag ttgcctaatg agttgtggtc gcaaagtgta aatcctttgt
     1381 atcatttcta cttcttttac actaaatcaa gcaatatctg agctaaaaat acttatataa
     1441 aaaataattt ccttgtaaat gctaggattg tcttcatcat aaactctaat tttgtaatcc
     1501 aagttatttt tatgaaattt aataaaatca taaaaacttt tttgcccata atcataagtt
     1561 ttaaattgat atcacttagc atagcttgaa tttatagtaa agtatgtaga agaagtagtt
     1621 ttggtccagg aagagctagc agatgtttta cttctgtaat aaacatcaaa cttaatttta
     1681 ttggtataat ttgtcaaaat attattatta ctatctctag cttttaccac taaatcaacc
     1741 cattgatttg tgcttggatt agatttgttt gtagtaagtt ggaacttgtt caaagtaggg
     1801 cttgtaacag ttgaagatca ccaagttcat gcagaaaagt tataatttct ttctattatt
     1861 ttccgatttc agttaaattg tgtatagatt tttaccaatc attgactagc tcagttccaa
     1921 gtaaagcttc cattacaagc tcaaatttct tttaactcac tatttggtaa caatattgta
     1981 caagctagag agcatcatcc accaggacag tttacaccgt ttgacatagt ataaacattt
     2041 ccatctttgt ttatactcaa acaagcagta tttcagcctc cataatttat tgtatggcaa
     2101 tctgtagcaa aaacatttga aaatcaaaca aagaatatta ctcacagcaa agatagtatc
     2161 aaagttaata attttttcat cttttttgat ttagtttata aaacaaacag aataaagccc
     2221 ctcactaaat atagagattt ttagttgata tgcaaatgag aatttatata agatttatag
     2281 tttttacttt ttatcaaaat tcaatgaaaa tagtatcaaa aatcataact tggattttat
     2341 ctatcatttt tctttttata ataataaatt tggcgattgg agttgtgaaa tatgagtgat
     2401 tagcaaatta ttccgatttt ttaaatcaaa aagagcgaaa tactgtggtt tctcagatct
     2461 ctataaaaga tccaatatct atatttagtt tgttttattg agaatacact ccaagagaaa
     2521 aaaatattga tgttcggcaa gaaaatttag aagacaaaat tatagaccaa gaacttcaaa
     2581 aggattcaca agtagacgaa gaggcaaaac tgaatgttta cgatccagat tttgaagatg
     2641 attttaatgc tttttttggt aaaacacata tttcaaaaaa aaagtcagac ttaattaatg
     2701 aagagggatg agcatttgta gtaccagaaa cttcagaaca agacaactta aatcaaacta
     2761 attcaaaaca ggataatgcg aacacaaaaa cagtttgaca gcaattgtta gaaaaattta
     2821 gtaaataaag aaatcaatgt tacaaactat aaacaatcaa gaagatacag ttttgtcttg
     2881 atcaaaaaat ctaattcaaa aatctttctc tgaaaataca aaaaacacta ttcgcttggt
     2941 agcaaacaca gatttccact atttgttatt agctgaaaat agtgaatcaa agattatttt
     3001 tgaaacaaaa tgagaaaata taaaatgaaa catatatgct atatattttg gctccaagcc
     3061 tataaaatca gacatacaag taaacatttc gtcttctaat gttgatataa atgtttttat
     3121 gttatctttg ttagtgtcag atcaagaatt taatacaaac tgaaatattt atttattaaa
     3181 agatattcat aattcacaag ggcatttact acaaaaaaat ctgattttgt gagaaaaaat
     3241 aaaagttagc tctatcccca gattagatgt ttattccaat gatatcaaag catcacacgg
     3301 atttagtatt gatagactta atcaggataa gatgttttat ttgaatagta aaggcttgtc
     3361 agaatccact tccaaagagc ttattgttca ttgaaacatc aaagatattt taaataaatt
     3421 cacaaatcta tcagaagaac aaaaaaatgc aatagaagac ctgattctat caaaaaccta
     3481 gtattctttt tgaaaaatat tatttattac aagtcttcaa tccaaagata tagaacgaca
     3541 aaacatttta cctacaaaac ccactatgga aaagtatttc aacctattat tagatttact
     3601 agagataaaa caaaatcaaa actactcaaa aattctaaaa attatccaaa aaaataaaga
     3661 taaaaaaata tttttttatc atcaatatag ttttattttt gagtgatatc aaaaactatt
     3721 aaatcaagaa ttagcactag aggagttttt acaaatctga gatataaatt ccaaactcaa
     3781 aattgattcc aaaaaaatag actctatttc ttttttgcaa aacaagctaa aaatagatcc
     3841 aaattctatc tacattttcg actccattgc tcaatgattt ttgtcagaac aagcaaatat
     3901 attttattgt taaaatagtt tggttaaggt cataatatac caagaaacat cactctcccc
     3961 ttctaagtaa tgtttattta gctaattaaa tttctactca ttccaatttc agcaattttt
     4021 ttttattttc caatcatcat caataaccaa ctaaatctca attatgtcca attactctat
     4081 gacaaggaat aaaaatagct atcggatttt tgttgtttgc cataccaaca gcacgcatag
     4141 cttttggaat tccaatagtt tctgcaattt ctttgtagct tttggtcttt ccataaggaa
     4201 tattttgtaa agccttccag acacttttca taaaatctgt tccttgtgga tttaatggta
     4261 atgaaaattc ttttattttt cattcaagat atttttctaa ttgcaaaaaa gcatctctaa
     4321 gtagaggagt ttctttgtat tcataagtat ttacaatatt ttttgggtcc caaaaaagat
     4381 gagttatttt tttatcttgt tcaacaatac ttattttacc aagcacagtc tgagccgaaa
     4441 aaatatatcc cattttctaa tcatctataa aatttctaaa atcttccaaa ctatcaaaag
     4501 acttgtacac agaagcgaat ctaatatata ccacaggatc tatctctttt agcatttcca
     4561 aaatatcatc tccaatttgt tttgaagaaa tttctgtccc ttcgcttatt cgcttatttt
     4621 ccaaattatt caaaagattt tctatttttt cgttattaaa ttctcttttg gcaaaagaca
     4681 gcatcaaaga ttttttcagt ttttgtctat catacat
//
LOCUS       DAOSID010000059         4655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_16379_length_4655_cov_13.594783, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000059 DAOSID010000000
VERSION     DAOSID010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16379_length_4655_cov_13.594783"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..918)
                     /locus_tag="PK674_02870"
     CDS             complement(154..918)
                     /locus_tag="PK674_02870"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HOG15508.1"
                     /translation="MNKKDNSDFSSKMKALNDLIQNSRNIVFFGGAGVSTESGVPDFR
                     SKDGLYNQHDIQFDHYQPEYLLSHSCLYNESQVFFEYYRQKLDCRRIKPNITHYKLAE
                     MERTGKLLAVITQNIDGLHQSAGSKNVIELHGTTQRVYCDTCNKNYNSDFLFTDKRAI
                     PFCTCGGIVRPDVTLYEESLPSDAWNSAMQAVAKADLFIIGGTSLTVYPANTLVQFFR
                     GKHLVIINKDPTPQDQFAELVFHENLGKVFEKVEIR"
     gene            1165..2709
                     /locus_tag="PK674_02875"
     CDS             1165..2709
                     /locus_tag="PK674_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15509.1"
                     /translation="MNINRENYIIFLTILLFRTVFLFTEAVFSHKEKNIEIKSSPIVV
                     INENKDIEEKQHYSPDIKEVQKSIIPKVQDLEELYKKIEKENKEEKLDFKEISLKDIG
                     GSLDGIEILEKLYVKTKTSDVLKVLVDKLLLDYQFEKAKFYLADLDMLKNSIIGARSY
                     FYTQINTLSITDPQSMNKFVNLVDQMKYRSMISVDDHLFYQGLAKIWKKEYDSVVEVF
                     KQIQSPIYKNIISQIEDTIVKFNNQKGAPAYYKDSLIALVMMKNGYFSIANKLAVESL
                     LQNKDYILPYQILAYSNFLTNNREKAIENFYTLISLDIGNKNKYNFYIGISQYWLGEY
                     TKSILTLSQITSDPTYKMDSYRYLLLNYQKLDDDEKMVQIWQKLLGQDALKESDFQTF
                     YDFVFFKDLGNKRKSSIYKKYKQLAYDYVFACYEQFGQSNDTCLYGEVGLNIVNNNLE
                     VVENGLIYLSEKYPQFHIFQALGDFYKNNKQSDKAKTYYLKAISLSKNTFQKNMIEQN
                     LIETLN"
     gene            2815..4068
                     /locus_tag="PK674_02880"
     CDS             2815..4068
                     /locus_tag="PK674_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15510.1"
                     /translation="MKNSLNRLGNTVAIPIRLGGTIVTTSADLFTTLTTSLKEGCEIV
                     ANTSQEIKNVLLGAWNHGKWYHKALNVPLSPIVAIGTAFEGIVRSAVQPVISGIVNTW
                     NTGINTIKNARKGSFGRLFSKKPLSDFSYNHLKTRPLTLNNWFAKLQFARGKSEGGES
                     KGAVAVLPDKKKSEKKQEGESSEEKVEKKLEKTKEEKKFEKVEEKKSKEKNKEEVREK
                     NKEELDKEAGYKQAEEILKDSKHGSKIYKKIRYNYPGLAFIFDKNLSSGEIKGDENKI
                     IVGTKLPSDEKQIAPLNDKKDNKEQIKHVLLHEMSHMIIDNNLGKVERVLDISKNVFE
                     RKQTTLTPLAQLGIYKTPISKAKEDLSEMMALYANGKLDEYLGKLTSDKKEDEEYREK
                     FKLAKISNDDAKVIKNTCEKLVSEL"
     gene            4367..>4655
                     /locus_tag="PK674_02885"
     CDS             4367..>4655
                     /locus_tag="PK674_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15511.1"
                     /translation="MILLEGKSLSQKIQEELVIKTKKFFPNNDKYIGIIYLGNNESSK
                     IYVGLKQKYGEKIGIKGYVFGQYKKGDKLPENTKEDYDVQLYMKQEYDNIGK"
BASE COUNT         1836 a          525 c          736 g         1558 t
ORIGIN      
        1 tcgtgatgaa gcacatagat ttgccaactc ttatagaaaa gagcagatga aaaaaggctt
       61 ataatttata gagtatgaga taattatgat aaaataagta gaaatttata tttgacatat
      121 tatatgagat tgatcagtag attattgatt tttttatctt atttcaactt tttcaaaaac
      181 cttccccaag ttctcatgaa aaactaattc agcgaattgg tcttgaggag ttggatcttt
      241 gttgattatt actaaatgtt ttcagcggaa aaattggact aaggtattag caggataaac
      301 tgtcaatgaa gttcacccaa tgataaaaag gtcggcttta gcgacagctt gcattgcact
      361 attccaagca tcacttggta aactttcctc ataaagtgta acatcaggtc tgactatacc
      421 tccacatgta caaaaaggaa ttgctctttt atccgtaaat aaaaaatctg aattatagtt
      481 tttattgcaa gtatcacaat aaactctttg tgtagtcccg tggagttcta tcacattttt
      541 acttcaagca ctttggtgta atccatcaat gttttgagta attactgcca aaagtttacc
      601 agttctttcc atttctgcaa gtttgtaatg cgtgatatta ggtttaatcc ttctacaatc
      661 cagtttttgc ctataatatt caaaaaaaac ctgagactca ttgtaaagac aagaatgact
      721 caataagtat tctggttgat aatgatcaaa ttggatatca tgttgattat aaagtcagtc
      781 tttacttcta aaatcaggaa ctcaactttc agtagaaact catgcacctc agaaaaaaac
      841 aatattacga gaattttgga ttaaatcgtt caatgccttc atctttgaag aaaaatcaga
      901 attatctttt ttattcattt ttgttggttg atataaatca atactcatta taagtaaaat
      961 ttgtctttat tccatagttt tcttttattt gcaataataa aaatagccaa gtttttgaaa
     1021 tgtatcaaag tatatctagt tttaaatata aattattctg tttgtcaatg tagactactg
     1081 tgcattttgg tagttttttt gataaattta cttttctgat tataattagt tatgtaaatt
     1141 taaaaataat actatcatat tgaaatgaac attaataggg aaaattatat tatatttttg
     1201 accatcttat tgtttcggac agtattttta tttacagaag ctgttttttc tcataaagaa
     1261 aaaaatatag aaataaaatc aagtcccatt gtggtaatta atgaaaataa agatattgaa
     1321 gaaaaacaac actattctcc tgatattaaa gaagttcaaa aatcaattat tccaaaagtt
     1381 caggatctag aagagctgta taaaaagata gaaaaagaga acaaagaaga aaaattggat
     1441 tttaaggaaa tctctttgaa agatatttga ggaagtttgg atggtataga aattttggaa
     1501 aaattatatg tgaaaaccaa aacaagtgat gtcttgaagg ttttagtgga taaactttta
     1561 ttggattatc aatttgaaaa ggcaaaattt tatcttgctg atttggatat gctaaagaat
     1621 tcaattatat gagcaagatc gtatttttat actcagataa atacattgtc catcacagat
     1681 cctcagagca tgaataaatt tgttaatttg gtggatcaaa tgaaatatag atcaatgatt
     1741 tctgtggatg accatttgtt ttatcaatga ttggccaaga tttggaaaaa agaatatgat
     1801 agtgtggttg aagtattcaa gcaaattcaa tctcctatat ataaaaatat tatttctcaa
     1861 atagaggata ctattgtgaa atttaataat caaaaaggag ctccggctta ttacaaggat
     1921 tcattgatag ctttggttat gatgaaaaac tgatattttt ctattgcaaa taagcttgct
     1981 gtagagtctt tgttgcaaaa taaagattat attttacctt atcaaatttt ggcttattct
     2041 aattttttga ccaataatcg agaaaaagct atagaaaact tttatacttt gatatctttg
     2101 gatataggaa ataaaaataa atataatttt tatatttgaa tttctcaata ttggcttggt
     2161 gagtatacaa aatctatttt gacattatcg caaattacga gcgacccaac ttataaaatg
     2221 gactcctata gatacttatt gttgaactat caaaagcttg atgatgatga aaaaatggtg
     2281 caaatatggc agaaattgtt atgacaagat gctctaaaag aatctgattt tcagacattt
     2341 tatgattttg tctttttcaa agatttgggg aataaacgaa aaagtagtat ttacaaaaaa
     2401 tataagcagc tagcttatga ttatgtgttt gcttgttatg aacagtttgg gcaatcaaat
     2461 gatacttgtt tatactgaga agttggtttg aatattgtaa acaataattt ggaagtagtg
     2521 gagaacggtt tgatttattt atcagaaaaa tatcctcagt ttcacatatt tcaggcactt
     2581 tgagattttt ataaaaacaa taaacagtca gataaagcga aaacttatta tttgaaggcg
     2641 attagtcttt caaaaaatac ttttcaaaaa aatatgatag aacaaaattt aatagaaaca
     2701 ttaaattaat ctcttttttt gttaccttga tttttttatt acattttctg ttccattttt
     2761 tgacaaataa aaaaaaatta ttaaaatagt aatactttat attatttcaa taatatgaaa
     2821 aacagtttaa atagattagg gaatacggta gcaataccga ttaggttgtg atgaaccatt
     2881 gttaccacat cggctgactt atttactact ttaactacat ctttaaaaga gggatgtgaa
     2941 attgtagcaa atacaagcca ggaaattaaa aatgtattat tgtgagcttg gaatcacggg
     3001 aaatggtatc ataaggcatt aaatgttcct ttaagtccaa tagttgctat atgaactgcc
     3061 tttgaatgaa tagtcagaag tgctgttcaa cccgtaataa gttgaattgt aaacacttgg
     3121 aatactggta tcaatactat caaaaatgca cgaaaaggat cttttggaag acttttttct
     3181 aaaaaacctc tttcagattt ttcttataat catctaaaaa ctagaccact cacattaaat
     3241 aattggtttg ctaaattaca atttgctaga tgaaaatctg aatgatgaga atcaaaatga
     3301 gctgttgctg ttcttccaga taaaaaaaag tcagaaaaaa aacaagaagg ggaaagttct
     3361 gaagagaaag ttgaaaaaaa gcttgaaaaa acaaaagagg aaaagaagtt tgagaaggtg
     3421 gaagaaaaaa agtctaaaga gaaaaataag gaagaagtta gggaaaaaaa taaagaagaa
     3481 ttggataaag aagcctgata caaacaggca gaagaaattt taaaggattc taaacattga
     3541 agtaaaattt ataaaaaaat acgctataat tatcctggat tagcttttat ttttgataaa
     3601 aatttaagta gttgagaaat aaaaggtgat gaaaataaaa ttattgtttg aactaagtta
     3661 ccttctgatg aaaaacaaat tgcaccttta aatgataaaa aagataataa agaacaaatt
     3721 aagcatgtat tactacatga aatgtcacat atgataatcg ataataattt atgaaaggta
     3781 gaaagggttt tggatatcag taagaatgtt tttgaaagaa aacaaactac cctaactcct
     3841 ttagctcaat tgtgaatata taaaactcca ataagtaaag caaaagaaga tttgtctgag
     3901 atgatggctc tttatgcaaa ttgaaaattg gatgaatatt taggaaaact aacatccgat
     3961 aaaaaagaag atgaagaata tagagaaaaa tttaaattag ctaaaatttc aaacgatgat
     4021 gctaaagtga taaaaaacac ctgtgaaaaa cttgtttctg aattgtagtg aggtaaaaat
     4081 aatcaaaatg gatgcaaaac catctggcga aaaaagtaag gctacataga gattattatt
     4141 ggattattaa aatcaatgtt gtaggggtga ttaataagta acctttacaa aagatgtaat
     4201 agtttgtttt aaataataat gtaggggaca gtgactgttc tttataaaag atatgtaaat
     4261 ttctattaaa attaaagaaa aacaattgat tcttgctttt tatagagaaa aaaatataag
     4321 atgattccgt tttaatagtg attttttata aataaagata ttgtttatga ttttgttaga
     4381 atgaaaaagt ctatcacaaa aaattcaaga agaattagtg ataaaaacca aaaagttttt
     4441 tcctaataat gataaatata tttgaattat atatttatga aacaacgaat cttcaaaaat
     4501 ctatgtgtga ctcaaacaga agtattgaga aaaaatatga ataaaatgat atgtttttgg
     4561 acaatataaa aaatgagata aattgccaga aaatacaaag gaagactatg atgttcaatt
     4621 atatatgaaa caagagtatg acaatatttg aaaaa
//
LOCUS       DAOSID010000060         4575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_16691_length_4575_cov_18.332743, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000060 DAOSID010000000
VERSION     DAOSID010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16691_length_4575_cov_18.332743"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1024)
                     /locus_tag="PK674_02890"
     CDS             complement(104..1024)
                     /locus_tag="PK674_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="translation initiation factor eIF-2B"
                     /protein_id="HOG15512.1"
                     /translation="MSKFDNEILQVADDIRTITIQGATNIAREACKVMEKQLRLKKFS
                     NKKELKDFFDRSTKLLIEARETEPMLRNGMKYARHKLKAGANAIELADAFAEYLTWIN
                     EEEKIRAEIGVNLIDDGDNIMTHCHSGSVTKILKTARNEGKKIHVYNTETRPLYQGRK
                     TSTKLVEAGVPNTMVTDSSAPFFVDNIYEKDIEINKVFLGADSIQSNGDTINKVGSFA
                     IALSAWHSGIPLYVVGSLLKLDISGEIGIETRDGKELRSDAPEGLKILNYAFDMIPAK
                     CITGIITEFGIIRPENLLKEVKKRYPWMLE"
     gene            complement(1378..4074)
                     /locus_tag="PK674_02895"
     CDS             complement(1378..4074)
                     /locus_tag="PK674_02895"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HOG15513.1"
                     /translation="MYYKKSIEDIAQEFQVDTKIGLTDKQIKENQKKYGLNILTNEKK
                     VKAWKVFLNQFKSFLILVLIAAAVVSIFIGEMVDSIVIISIVILNAILGFVQEYNAEK
                     SIESLKKMSGQMSRVIRNGREILIDSKQLTVGDILVFDAGDKIGADARIIQSISLELA
                     EAVLTGESLPVKKHVNPINQDSALGDQKNMIFAGTDVTKGHGRAIVVSVGMQTEIGKI
                     AYMIQEAPEPQTNLQKDLDKLGKRLVWIILLICVIVFISDVFIVKDSRQNALLMAIAL
                     SVAAIPEGLPAVVTVSLGLGVKRLVKKNALMRKLPSVETLGSVNVICTDKTGTLTKNA
                     MTVKHIFVDNKVFDLDGVGYQDFANKDFLIKNKNNENSLREILKIGVLCNNSSINGVD
                     IVGDPTEIALLVSAMKGNIERMILEKEYELIDEIPFDSERKIMTSIYKKISFSEFIEK
                     KDNKIYLYSKGAPEVLLDKCTHFLTNGEVRKLTEKDKKNILSQNKKFAESALRVLGFA
                     YRIVENYEFNQVYKTESGNVSALEENFVFVGLQAMIDPPRVEVKDSIQICKNAGIRVV
                     MITGDNVTTAKAIAEQLGIDGMTMDGVELDKISDRNLAKIIDKIGVFARVSPAHKQRI
                     VTLLKEKGNIVAMTGDGVNDAPALKVADIGLAMGITGTDVSKEASDMILLDDNFATIV
                     NAIYEGRGIYDNIKKFVNYLLSTNFAEVLIIFVSVLLGMPLPLIAIQILMINLVTDGL
                     PALALGIDPVSPDVMHRKPRKLGSKIIDRKMLTSIIYLSILMTIVILFLFFRHYHVDL
                     EQARTGVFVLLVLLEMIRIWMIRSEYKLKFFSNKWLLGAIVGSIALVLLIVYIPSLSK
                     IFHTKALSLWIWIEILLILFVLFIGVKISKFLKLKFYKLKNG"
BASE COUNT         1580 a          846 c          513 g         1636 t
ORIGIN      
        1 catttcaaca ttttatcatt catcccgcta agcgggacaa gtttcaacat tttatcattt
       61 tatcatttca acattttatc attttatcgt gagcagagcg aacttactcc aacatccaag
      121 gatatctttt tttcacttct ttaagtaaat tttctggtct aattattcaa aactcggtaa
      181 taatccctgt aatacattta gcaggaatca tatcaaaagc atagttcaaa atcttcaaac
      241 cttctggagc atctgatcga agttccttac catctctagt ctcaattcaa atttcccctg
      301 aaatatcaag ttttaataaa cttcaaacaa catataatgg gatacctgaa tgccaagccg
      361 ataaagcaat agcaaaactt caaactttat ttatagtgtc tccattagat tggatagaat
      421 ctgctcacaa aaagaccttg tttatttcaa tatctttttc ataaatatta tccacaaaaa
      481 atggtgctga actatctgtt accattgtat ttggaacacc agcttcaact aatttcgtag
      541 aagtctttct tcattgatat aatggtctag tttctgtatt gtaaacatga attttttttc
      601 attcatttcg tgctgttttc aaaatcttag tcacagatcc tgaatgacaa tgagtcataa
      661 tattatctca atcatcaatc aaatttactc ctatttctgc tctaattttt tcttcctcat
      721 ttatccaagt aagatattct gcaaaagcat ctgccaattc tatagcattc gctcaagctt
      781 ttaacttatg tctagcatat ttcattcagt tacgaagcat cggctctgtt tctctagcct
      841 ctatcaaaag ttttgtagat ctatcaaaaa aatcttttaa ttctttttta ttgctaaatt
      901 tttttaaacg caattgtttt tccattacct tacatgcttc tctagctata ttggtagctc
      961 attgtattgt aattgtacgg atatcatctg ctacctgtaa aatttcatta tcaaatttag
     1021 acatttttta tttttatgat ttaaaactac attcaattat atatgatttt attttttttg
     1081 caaattttgg gactcttatt tttttaaact attctattgt ttataaataa attattgtaa
     1141 aagcttcttt gtgaataata tataaagcat aataaagcat aatttttcta tttttatttt
     1201 caaaaaccag catatctctt ccttacaaaa ataaaacgaa gttcagaatt tatcaaagat
     1261 aaagaaaagt aagtgttaaa gcttttttta ataagaaagt tttagataat ggattcatgt
     1321 tagagagttt taaaaaataa aaataaggtt ctgaaacaat aaattactca ataaacttta
     1381 tcaatttttt aatttataaa actttagttt caaaaacttc gaaattttca ctccaataaa
     1441 taacacaaat aatatcaata aaatctctat ccaaatccat aaagacaaag cttttgtgtg
     1501 aaatatcttt gataaacttg gaatatagac tatcaataaa actaatgcta ttgatcaaac
     1561 gattgctcac aacaaccatt tatttgaaaa aaactttagt ttatattcac ttctaatcat
     1621 ccagattctg atcatttcca acaaaaccaa taaaacaaaa actcatgttc tagcttgttc
     1681 caaatctaca tgataatgtc taaaaaacag aaataaaata actattgtca taagaatgga
     1741 tagataaata atacttgtta acatctttct atctataatt tttgaaccaa gttttctagg
     1801 tttacgatgc ataacatcag ggctcacagg atctattcac aaagccaaag ctggtaaacc
     1861 atcagtaact aaatttatca taagtatttg tattgctatc aagggcaatg gcatacccaa
     1921 taaaacactc acaaaaataa tcaacacctc agcaaaatta gtagacagca aatagtttac
     1981 aaatttctta atattatcgt aaattcatct tcattcatat attgcattta caatcgtagc
     2041 aaaattatca tctaaaagaa tcatatcgct tgcttctttg ctcacatcag tacccgtaat
     2101 tcacatagcc aatcctatat ctgccacttt caaagctgga gcatcattta ccccatctca
     2161 agtcattgca acaatatttc atttttcttt taatagtgtc acaattcttt gtttatgagc
     2221 tggactaact ctagcaaaaa ctcaaatttt atctattatt ttagctaagt ttctatcact
     2281 aattttatct agttctactc catccatagt cattcaatct attcccaatt gttctgctat
     2341 tgctttagct gttgtcacat tatctcctgt aatcattact actctaattc ctgcattttt
     2401 acaaatttga atagaatctt ttacctctac tctcggtgga tctatcatag cttgtaatca
     2461 tacaaacaca aaattctctt ccaaagcaga aacatttcaa ctctcagtct tgtaaacttg
     2521 gttaaactca tagttttcta ctattctata agcaaatcat aaaaccctta aagcagattc
     2581 agcaaatttt ttattctgtg aaagtatatt tttcttatct ttttctgtta gttttcttac
     2641 ttctcaattg gttaaaaaat gtgtacactt gtctagtaaa acttctggag ctcatttaga
     2701 atacaaataa attttattat cttttttttc tataaattct gaaaaggaga tttttttata
     2761 aatacttgtc ataatttttc tctcggaatc aaaagggatt tcgtctatta attcatattc
     2821 tttttctaaa atcattcttt ctatatttca tttcattgca ctaacaagca aagcaatttc
     2881 agttggatca cctacaatat ctactcaatt gattgaagaa ttattacaca aaactccaat
     2941 tttcaagatt tctctcaaag aattttcatt gtttttattt tttatcaaaa aatctttatt
     3001 agcaaaatct tgataaccca ctcaatccaa atcaaaaact ttattatcca caaaaatatg
     3061 tttaactgtc attgcatttt tggtcaatgt tccagtttta tccgtacaaa taacattcac
     3121 ggaaccaaga gtttccacac tcggaagttt tctcatcaaa gcattctttt tgactaacct
     3181 ctttactcca agtcacaaag agacagtaac tacagcaggc aatcattcag ggatagctgc
     3241 aacagacaaa gcaattgcca tcaaaagagc attttgtcga gaatctttta ctataaaaac
     3301 atcactaata aaaactataa cacaaatcaa caaaattatc catacaagcc gtttccccaa
     3361 tttatctaaa tctttttgta aattagtttg tggctctgga gcttcttgta tcatataagc
     3421 tatttttcat atttctgttt gcattcaaac tgacacaaca attgctctcc catgtcattt
     3481 tgtaacatca gttcatgcaa aaatcatgtt tttttgatct cacaaagctg aatcttggtt
     3541 tataggattc acatgttttt ttacaggcaa ggactcccct gtcaatactg cttccgctaa
     3601 ttccaaacta attgattgga tgatacgagc atcagctcaa attttatctc ctgcatcaaa
     3661 aactaaaata tctcacacgg tcaattgttt ggaatcaatc aaaatttctc tcccatttct
     3721 aatcactcta gacatttgac cagacatttt tttcaaagat tcgatagatt tttctgcatt
     3781 atattcttga acaaaaccaa gtatagcatt gagaataaca atagagataa tcacgatact
     3841 atcaaccatt tctcatataa aaatggaaac aaccgccgct gctatcaaca ccaaaatcaa
     3901 aaaacttttg aattgattta aaaatacttt ccaagcttta actttttttt cattagtcaa
     3961 aatattcaat ccatatttct tttgattttc tttaatttgt ttatcagtca atcaaatctt
     4021 tgtatcaact tgaaactctt gtgcgatatc ttctatagat tttttgtagt acatattttt
     4081 tgtttttgaa acttctaaaa aagtcttacg aaaacaatta tattcccaaa acaaaaaata
     4141 gcaaaaattt tataaacaaa caaacaattt aacttgacaa accaatcaat tataaattga
     4201 taaataagta ccacccacca cccggccccg taggactacg gggcgggtgg atgggtaaaa
     4261 aaagttaaaa aacaattgaa aaacaaagga aaatagctat aatcaaagtg tatggggatg
     4321 tacaggtttc gataaaagat tctcatatct aatagttgcc actactcgat gagcgtaccg
     4381 agtctaaaat aatcgcttag catctaaaaa tgcaaactca aaaaatttgt tagcaagagc
     4441 taacgctttc gtattaaact acgcctagtt ttttgttcga ccaccatcgc aagatggatg
     4501 taagaacaat gccggttata acatcggcat acgatcgtat caaccggttc taccagacac
     4561 ctactaggta gacgg
//
LOCUS       DAOSID010000061         4556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_16794_length_4556_cov_14.234837, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000061 DAOSID010000000
VERSION     DAOSID010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16794_length_4556_cov_14.234837"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            509..2245
                     /gene="dnaG"
                     /locus_tag="PK674_02900"
     CDS             509..2245
                     /gene="dnaG"
                     /locus_tag="PK674_02900"
                     /inference="COORDINATES: protein motif:HMM:NF019880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="DNA primase"
                     /protein_id="HOG15514.1"
                     /translation="MANVAEFILDNIDIVDVVSRRVNLKRAGSNFAGLSPFQHEKTPS
                     FMVSPQKQIFKDFSSGIGGNAISFVMEYEKIDFRDAVKLIAEEHRLDISKYTKAYNYS
                     KDFVDEKEKIKRIHKLAQNFFTDNLNKSPNALDYLQNQRKLSPQIIKNFGIGYAPNSN
                     YDLITKLKEKGFNEQDLIQASLIKKGQSDFYSFFRNRITFPIFDTRNNIVGFSARVLN
                     PEDQPKYLNSSEHIAFEKSKILYGINFAKDSIKVHNMLILVEGQMDVIALARLGLPIG
                     VATCGTSVTAHHFKLMKRYTENIYILFDNDDAGKNATFNALKIAYRENMYPKMISLPK
                     EFKDIDDLANIETGLDVFNTCIKDSKDAFLQIYELLRIKYDMSSPIDKQKLFNDMFEL
                     IANVDNYTIQEHYKQLLAEKVGLPYEVVSLQFRKYTQTDGKFELVRKERKEEKQTWKP
                     DRESLSAALFYENIFEKYIEDKENWTNLILSVKSIGKYRENSILQKVLEKTNSLSQEE
                     IKELNEIQLRWDKELTDLNGDEKKFISIKNVVLPVLNTGLKIILKNPNITHEIKENLR
                     NSMKYAGKYGSS"
     gene            complement(2421..2849)
                     /locus_tag="PK674_02905"
     CDS             complement(2421..2849)
                     /locus_tag="PK674_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15515.1"
                     /translation="MFKKLLLGLGIAGLLGVGSIALVNAQNFEPGQFGDVGGTAGVGV
                     SGAGVDKGGSLIQVIKNFINRILGLLALITLVIVLRGGFKMVTAAGDEGKFKEGFKIL
                     KQAGIGLAIIGLSWFVVSIIFRVISGSSANVDGGGGTTGG"
     gene            complement(2960..4087)
                     /locus_tag="PK674_02910"
     CDS             complement(2960..4087)
                     /locus_tag="PK674_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15516.1"
                     /translation="MKKFYLFLFILFSAFLFSFTKAQVELSPVYSSDRFQPSDKFHAG
                     CENQLDVIFSLNYSNVVGVNAILEYNSDEIEILKILPEGEKENNLSYVVESNKIIFNK
                     LKTQDSGLQRAVFKMFFRAKPTLFFTDFNFSTGSYVLDTKGNMVELNKSYNFKFQRVP
                     ECEPDIIAPTIELLFPILQSGEYIALDSHYQFDISDLGKGINKDKINFTIDDIKYDLS
                     NTEHERNGDILTIYPSIWLPLGSTVKLNIEVSDKQVYGKANTTRKEYIFQTSNELNLL
                     NEIDPVQFRKLVNKEKYYQGSQAECKWLEEQFLISEDSIKDTILSINKRLSCADLDGL
                     SGLLNLQEIDDKVGFSVFSTLGRLLFGLLLFLLIFKRLSKK"
     gene            complement(4121..4483)
                     /locus_tag="PK674_02915"
     CDS             complement(4121..4483)
                     /locus_tag="PK674_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15517.1"
                     /translation="MKNQVNPFQVKVLSFGSDGKVNVQLPSTIEVDGEIITLKTREIS
                     VDTGKNPDHNIRRDIRKVLREKHGICVPFREQDNTIIINGIFTHSGPYNVSYFFIKKF
                     LLMIISFSLDNLSVNNKQ"
BASE COUNT         1788 a          708 c          580 g         1480 t
ORIGIN      
        1 aattttagag ctagagagta caatggtaga tggtacaatg ggatctctgc atggagaata
       61 gatggtattt gaactgcgac tgcatctaca ccaaaccctt ctgcaccagc agatgatgat
      121 ttgccattct aaaattaaaa actataaaaa ttatacaaaa aagccttaca aaattaattg
      181 taaggctttt tatcaaacaa tttagagata attaaattta tatacgaaca gaaaaatacg
      241 gataaaaaaa ctgtatccta tcaagcagag tggaaattgt gccatattct cctacaatac
      301 tataaaatag caaaatatga aacgaagaaa aatctgacat atcaagaacg aataaggtaa
      361 agtcaaaatc ctaccctttt cacaacttat taaaattggc gctcatccta ttttctatta
      421 atgaattgaa aaatatgata gaataaatat atattctata caaaaatata taaaagtatt
      481 agtttttaaa aaacaagttt aaattcttat ggcaaatgta gcggaattta tattagacaa
      541 catagatatc gtagatgtag tttccagaag ggtaaatctc aaaagagctg gaagtaattt
      601 tgctggttta tcgccttttc aacatgaaaa aactccttct tttatggtat ctccacaaaa
      661 acaaattttt aaagatttta gctctgggat aggtggaaat gctattagtt ttgttatgga
      721 atacgaaaaa atagatttcc gagatgctgt aaaacttata gctgaagaac atagattaga
      781 tatttcaaaa tataccaaag cttacaatta ttcaaaagat tttgtagatg aaaaagaaaa
      841 aataaaaaga atacacaaac ttgctcaaaa tttttttaca gacaatctaa acaaatcgcc
      901 gaatgcccta gattacctac aaaatcaaag aaagctttca ccacaaataa taaaaaattt
      961 tggtattggt tatgcaccaa attccaacta tgatcttatc acaaaactaa aagaaaaatg
     1021 atttaacgag caagacttaa tacaggcttc tttgataaaa aaatgacaga gtgattttta
     1081 tagttttttt aggaatagaa ttactttccc aatatttgat actagaaaca atatagttgg
     1141 atttagtgct agagttttga atccagaaga tcagccaaaa tacctaaata gtagtgagca
     1201 tattgcattt gaaaagtcaa aaattctata tggtatcaat tttgcaaaag atagtataaa
     1261 agtccataat atgttgattt tagtagaatg acaaatggat gtaatagctt tggctagact
     1321 atgactacca atatgagttg ctacttgttg aacttcagtt acagcacacc atttcaaact
     1381 gatgaaaaga tacacagaaa atatatatat tttgtttgat aatgatgatg caggaaaaaa
     1441 tgcgacattt aatgcattaa aaatagcata tagagaaaat atgtatccaa aaatgatttc
     1501 cttgccaaaa gaattcaaag atattgatga tttagcaaat atagaaacat gattagatgt
     1561 tttcaatacc tgtatcaaag actccaaaga tgctttctta caaatctacg aattattgag
     1621 aataaaatat gatatgagtt ctccaataga caaacaaaaa ttatttaacg atatgtttga
     1681 attaatagca aatgttgaca attacaccat ccaagagcat tacaaacaat tattagcaga
     1741 aaaagtttga ttaccttatg aagtcgtttc tctacaattt agaaaatata ctcaaacaga
     1801 ttgaaaattc gaacttgtta gaaaagaacg caaagaagaa aaacaaactt ggaagccgga
     1861 tagagagtct ctttctgctg cattgtttta tgaaaacata tttgaaaaat atatagaaga
     1921 caaagaaaat tggacaaatc taattttatc agtcaaaagt atttgaaaat accgagaaaa
     1981 ttccatatta caaaaagtac ttgaaaaaac aaattcctta tctcaagaag aaataaaaga
     2041 actaaatgaa atacagctac gatgggataa agaacttaca gacttaaatg gtgacgaaaa
     2101 aaaatttata agtataaaaa atgttgtttt accagtctta aatacttgac taaaaataat
     2161 actaaaaaac ccaaacataa cacatgaaat aaaagaaaat ctaagaaata gtatgaagta
     2221 tgcaggtaaa tattgatcta gttaatctca aaaaaacaac taaccatgaa ataatataca
     2281 ttttaaaaaa cattatcttt caaatttaaa aataagatca agcaacaact atcctacttt
     2341 cacaaaaaat tcaattgaaa catataaata aaaaaatccc tgttgtgcaa acagggattt
     2401 tgtattataa cataatgttt ttatcatcat gtagtacctc atcatccatc aacatttgct
     2461 gatgaacctg aaataacccg gaatataata ctaaccacaa accaagacaa accaataatt
     2521 gctaatccaa taccagcttg tttcaaaatt ttaaatcatt ctttgaattt tccttcatct
     2581 ccagcagctg taaccatttt gaatcctcct cgtaacacaa taaccaaagt aataagagct
     2641 aataatccta agattcggtt aataaaattt ttaattactt gtatcaaact tccaccttta
     2701 tcaactcccg cacctgaaac tcccacaccc gcagtaccac ctacatcacc aaactgtcca
     2761 ggctcaaaat tttgtgcatt aactaatgca atacttcata ctcccaatag tcctgctata
     2821 ccaagaccta gtaataattt tttaaacatt ttttaaaact ttaaagataa aaattatatt
     2881 atatttaata tatgtgctga agataataat ttttgtacaa attgcaaatc tattttgatt
     2941 cccactttga tccccattct tatttttttg atagtcgctt aaatattaat aaaaacaaaa
     3001 gtaaaccaaa caaaagtcgc cctaatgtag agaatactga aaatcacact ttgtcatcta
     3061 tttcttgtaa atttaataag ccagaaagtc aatctaaatc cgcacaactt aatctcttgt
     3121 tgatagacaa tattgtatct tttatgctat cttctgagat taaaaactgt tcttccaacc
     3181 acttacattc tgcctgagat cattgataat atttttcttt attcacaagt tttctaaact
     3241 gaacaggatc tatctcgttt aataaattta gctcatttga agtttggaat atatattctt
     3301 ttcttgtcgt atttgctttt caatatactt gtttatcaga tacctcaata tttaatttta
     3361 ctgtagaacc caaaggtaac caaatacttg gatatatagt aaggatatca ccatttcgtt
     3421 cgtgttcagt attagataaa tcatacttaa tatcatctat tgtgaaatta attttgtcct
     3481 tatttattca ttttcataaa tcagagatat caaattgata atgagaatct aaggctatat
     3541 actctcaaga ttgtaagata ggaaacagta actctatagt aggagcaata atatctggtt
     3601 cacactccgg caccctttga aatttaaaat tataactttt atttaattcc accatatttc
     3661 atttggtatc caaaacataa cttccagtag aaaaattaaa atcggtaaaa aataaagtag
     3721 gctttgccct aaaaaacatt ttgaaaactg ctctttgtaa tcaagaatct tgagtcttca
     3781 acttattaaa aataatttta tttgattcta caacataact tagattgttt tctttctctc
     3841 attcaggtaa aatttttaat atctctattt catcgctatt gtactctaaa attgcattta
     3901 ctccaacaac attagaataa tttagagaaa aaatgacatc cagctgattt tcacatcaag
     3961 catgaaactt atctgatggt tgaaatctat cagaactata aactggactc aattctactt
     4021 gtgcttttgt aaaggaaaat agaaaagcag aaaatagaat aaataaaaac aaatagaact
     4081 ttttcatctt tttttgaaaa attaaagata aaacactcaa ttattgttta ttatttactg
     4141 ataaattatc aagagaaaat gaaattatca taagtaaaaa tttttttata aaaaaatagc
     4201 tgacattgta tggtcagcta tgtgtgaata ttcaattaat tattatagta ttatcttgct
     4261 ccctgaatgg gacacaaatc ccatgttttt ccctaagaac cttcctaata tcccttcgga
     4321 tattgtgatc agggtttttc ccagtgtcaa ccgaaatctc ccgagttttt agagttataa
     4381 tttccccatc aacctctatt gtggaaggca gttgaacatt gactttccca tcgctcccaa
     4441 acgataaaac ctttacttga aatggattca cctggttttt catagtttta ttattttttt
     4501 attacaatat aggaaattca aaaatctgat tttaggtatt ttttgttcgt gatatg
//
LOCUS       DAOSID010000062         4418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_17451_length_4418_cov_14.474215, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000062 DAOSID010000000
VERSION     DAOSID010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17451_length_4418_cov_14.474215"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PK674_02920"
     CDS             <1..809
                     /locus_tag="PK674_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=25
                     /product="type II secretion system F family protein"
                     /protein_id="HOG15518.1"
                     /translation="EKSGNLSIVLESLATEYEYIKEIKNKYIGALIYPIILIIIAVIA
                     VFALFGFVLPSVFDVANSFQGLELPAMTKLLKNMSDFFVENWKYIMYVFIVFLLILWL
                     FFSTDFGKKTQFKILFSIPVVGRMTKYYYLVRRCRYMKLMLVSGMNYVETFKTLRDIL
                     HISPYQTMIERVLAGLQRGEKIYDSLKHETNLIPGNVSVLIKVGEETANLDNSVDNIL
                     KMYQEELNMIINSLSKVIEPIMLVLIGIIVVIVASGVFGLILQIMEGAGM"
     gene            973..1500
                     /locus_tag="PK674_02925"
     CDS             973..1500
                     /locus_tag="PK674_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="type II secretion system protein"
                     /protein_id="HOG15519.1"
                     /translation="MKKLKAFTIIELLIVIAIIGILMATTMKFSGDRISFLNNKNIQE
                     QFVANFSSLQAQNNMTNYHFGKIYQDLKINFVLEEDHFTYSYHNHDSAIDTRATTTEG
                     GHYQINEIILDGDTTTEANIFMKPYTLGCTITNKDNNDGKNLKIGILVNDAKKYCFSI
                     ESNNCKIKTISCSEI"
     gene            1962..2615
                     /locus_tag="PK674_02930"
     CDS             1962..2615
                     /locus_tag="PK674_02930"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG15520.1"
                     /translation="MKKKYAFTFIEVMIAIVIFSVGILAVLSLVTNNLKSMDRNNLRL
                     QATLLAKEGIELVYNLRDANIEKKLSWNCLMNDEMYRRTAEELSNKIRKATQSDFEDI
                     ICEGYFGTGNALKIGFDSKNYLNYDNFQISNSFVDNFDKSRLFLLHNNNGLLRYGHST
                     GNTDKETYFARYLSFKTIKENNMVLPPDKILKVESHVLYMKGAYTGEVVFESFIGNY"
     gene            2616..3233
                     /locus_tag="PK674_02935"
     CDS             2616..3233
                     /locus_tag="PK674_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG15521.1"
                     /translation="MKKAFTLTEILIVTIIIGILVGVLFRTYITISQISFRVEQQKFL
                     NQEVLFVSETLQNFANKNKIDYHKYSQQGIDLHPTKGITDVLYLSGEDGKFSVYSTGA
                     CIALETAPTPSDLQKPCYLVVEKDGKFTRITESTVYISKVIFKIIPYADSQAYINNTA
                     LCESNYLACLHDHGFWFFSNFYSKGYSPANRSSNVTLFIQQFFNI"
     gene            3242..4144
                     /locus_tag="PK674_02940"
     CDS             3242..4144
                     /locus_tag="PK674_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15522.1"
                     /translation="MKNLFQIQKKIKGHISILIILVLLASSMIGLLTMNFLKQMVSYT
                     NETYSYHKSYYFAKAGLELSLTEIDNSGIGFSNTLSGSSPIFLNNFDCEYCGFDVNIQ
                     GKTKYLSDKFWLSKECDDDTAFLLKSRESFVLPMFSQKQVSSNLDTFSPPEYNFDLLK
                     YRKGLEFIQNQDYKGKINLGLIILSGDSVEKDYLLIKSHETSTDVLKIYFDEFDEFYG
                     SELLDKGKFPSFLLISNADDTDDLSFCIHSDNVIFGGVSQNVYLPTMNFYISSIGHYL
                     NRSVGLQAIYGQAIPGFLANTYLE"
BASE COUNT         1623 a          599 c          672 g         1524 t
ORIGIN      
        1 gagaaaaatc agggaattta tccatagttt tggagtcttt ggcgacagaa tatgaatata
       61 taaaagaaat aaaaaataag tatatatgag cattaattta tccaataata ctaattataa
      121 tagctgttat cgcggtattt gctctttttg gttttgtcct tcctagtgta tttgatgttg
      181 ctaatagctt tcaatgacta gaattaccag caatgaccaa acttttaaaa aacatgtcag
      241 atttttttgt agaaaattgg aaatatatca tgtatgtgtt tattgtgttt ttactcatat
      301 tatggttgtt tttttccaca gatttttgaa aaaaaaccca attcaaaatc cttttttcta
      361 ttcctgttgt gggtagaatg accaagtatt actatttagt tagacgatgt agatatatga
      421 aactaatgct agtttcaggg atgaattatg tagaaacatt caaaacttta agagacattt
      481 tacatatctc tccgtatcaa actatgatag aaagagtttt ggcatgatta caaagatgag
      541 aaaaaatata tgattctttg aagcatgaaa ctaatttaat tccatgaaat gtttcagtat
      601 tgataaaagt ctgagaagaa acagcaaact tagataattc ggtagataat atactaaaaa
      661 tgtatcaaga agaattaaat atgataataa atagcttgtc aaaagtaatt gagccgatta
      721 tgcttgtctt aatcggaatt atcgtagtaa tagtggcttc atgagtgttt ggtttgattt
      781 tacaaattat ggaatgagca ggaatgtaaa aagctcgctt tgctcgcgat aaagtgataa
      841 aaagttcgct tcgctcacga taaagtgata aaaagttcgc ttcgctcacg ataaagtgat
      901 aaaatgataa agtgataaag tgataagttg ttcattgtca attataagct ttacaaatta
      961 ataatccaga atatgaaaaa attgaaagcg tttaccataa tagaacttct tatagttatc
     1021 gcaattattt gaattttgat ggctactact atgaagttta gctgagatcg tatcagtttt
     1081 ttaaacaata aaaatattca agaacaattt gtggctaatt tttcttcatt acaagcacaa
     1141 aataatatga caaactacca tttttgaaaa atttaccaag atctaaaaat aaatttcgta
     1201 ttggaagaag atcattttac ttattcttat cacaatcatg attctgcgat agataccaga
     1261 gcaactacta cagaatgatg acactaccag attaacgaaa ttattttgga ttgagatact
     1321 accacagaag caaatatttt tatgaagcct tacacattgt gatgtacaat taccaacaaa
     1381 gataataatg attgaaaaaa tttaaaaatt tgaattttag taaatgatgc taaaaaatat
     1441 tgtttttcaa tagaatcaaa taactgcaaa ataaaaacaa tttcctgttc agagatttaa
     1501 aaacgaactt cgttcgtgat aaaatgataa aatgttaaag tgataaatat tcgccccatt
     1561 aagcggggtg aacttcgctc atgataaaat gataaaacaa tacacttgaa cctgtaggcg
     1621 cgaattcgaa ttcgcgcctg caatgttagg gaggatgtta aatccctcta ccccgcaccg
     1681 tagttcgcga taaagtgata aaacttgccc cgctaagcgg ggtgaatgtt aaagtgataa
     1741 aatgataaat gcctgcccag ccacatagta ctatcccgta gttttacggg acttacagga
     1801 cttggtggga tgaatgatga aatgattttt tatcaatcca ttattttaac aattattgga
     1861 tggggaataa tttttatatc ccaaataata attaacaatt ttttacttga taagtttgta
     1921 aagtttttta taagtactat gttttaatta aaaaattttt tatgaaaaaa aaatatgctt
     1981 tcacttttat agaggttatg atcgctattg tcatattttc cgttggaatt ttggcagttt
     2041 taagcttggt tacaaataat ttaaaatcca tggatcgcaa taatttaaga cttcaagcaa
     2101 ccttattagc caaagagtga atagagttgg tttacaatct tagggatgct aatatagaaa
     2161 aaaaactttc ttggaattgc ttgatgaatg acgaaatgta tcgtcggaca gcagaagaat
     2221 tatcaaataa aattcgtaaa gccacccagt cagactttga agatattatt tgtgaatgat
     2281 atttttgaac agggaatgct ctcaaaattt gatttgattc caaaaattat ttaaattatg
     2341 ataatttcca aatatccaat agttttgttg ataattttga caaaagtaga ttgtttttgc
     2401 ttcacaacaa taattgactg cttcggtatg gtcattctac tggcaatacg gataaagaaa
     2461 catattttgc cagatattta tcatttaaaa ccataaaaga gaataatatg gtattgcctc
     2521 ccgataaaat tttgaaagta gaatctcatg ttttatatat gaaatgagca tatactggtg
     2581 aagttgtttt tgaaagtttt atttgaaatt actaaatgaa aaaagcattt accctaacag
     2641 aaatccttat agttaccata attattggta ttttagtatg agttttgttc agaacatata
     2701 ttaccatttc tcaaatttct ttcagagtcg aacagcaaaa atttcttaat caagaggtgc
     2761 ttttcgtctc agaaacatta caaaactttg ccaataaaaa caaaatagat taccacaagt
     2821 attcccaaca aggtatagat ttacatccaa caaaatgaat cacggatgtt ttatatctaa
     2881 gctgagagga cggaaagttt tctgtttact ccacgggtgc ttgtatagca ttagagacag
     2941 ctccaacacc atctgattta caaaaaccat gttatctcgt tgtagaaaag gattgaaagt
     3001 ttacaagaat aacagaatct actgtttata tatcaaaagt aattttcaaa attattcctt
     3061 atgcagatag tcaggcttat atcaataata cagctttatg tgaaagtaat tatttagcat
     3121 gtttgcatga ccatggtttt tggtttttta gtaactttta ttccaaatga tatagtccag
     3181 caaaccgatc ctcaaatgtt acattattta tccaacagtt ttttaatatc taactttttg
     3241 tatgaaaaac ttattccaaa tacagaaaaa aataaaatga catatcagta tattgataat
     3301 tttggtttta ctagcctcat caatgatatg attattaacg atgaatttct tgaagcaaat
     3361 ggtttcatat acaaatgaaa cctactctta ccataaatca tactattttg caaaagcatg
     3421 attggaactg tctttaactg aaatagataa ttcttgaatt tgattctcca ataccttatc
     3481 tggatcaagt cctatatttt taaataattt tgattgcgaa tattgttgat ttgatgtaaa
     3541 tatacaatga aaaactaagt acttatctga caagttttgg ctatctaagg aatgtgacga
     3601 tgatacagct ttcttgctca aatcaagaga atcttttgtc cttcctatgt tttcacaaaa
     3661 acaagtttca tccaatttag atactttttc accaccagaa tataattttg atttgttgaa
     3721 atacagaaaa ggtctggagt ttatccaaaa tcaagattat aaatgaaaaa taaatttatg
     3781 attaattatt ttatccggtg atagtgttga aaaagattat ttactaataa aatcccacga
     3841 aacaagtaca gatgttttga aaatatattt tgatgaattt gatgaatttt attgatcaga
     3901 attattagat aaaggcaaat tcccatcatt tttacttatc tcaaatgcag atgatacaga
     3961 tgatttatct ttctgtattc attcagacaa tgtaatattt tgatgagttt ctcaaaatgt
     4021 ttatcttcct acaatgaatt tctatatttc cagtattgga cactacttaa atagatctgt
     4081 cggattacaa gcaatttacg gtcaagcgat tccgtgattt ttggcaaata catatttaga
     4141 ataattcctc taaaaaaata taaatttgga agcttaggtt ttttctgtaa taatacagaa
     4201 aaatcttttt ttgtaaaaat taaataaatc tttaaagatt atagtgtgtt tgctgaaaat
     4261 ataaaagata caagcactta acaaccaata ttaaaccaca aaacagtcaa tagtcaactt
     4321 gaattcaaaa aaagtcttca aaaacttgac aaaatggggg gggtgtacaa gttacttaat
     4381 tcggtaacaa attttttgta aaattaacag gagattgt
//
LOCUS       DAOSID010000063         4302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_18078_length_4302_cov_12.356958, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000063 DAOSID010000000
VERSION     DAOSID010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18078_length_4302_cov_12.356958"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            576..1856
                     /locus_tag="PK674_02945"
     CDS             576..1856
                     /locus_tag="PK674_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG15523.1"
                     /translation="MSKIKQQLREKLEDDITELTKPLQLLLNKGFTSGTVNEEDIISE
                     IDDMDMSSKLLEKFYELAEKLSIKVLTIEEILEKEAQALVEKSKLGKIELYETTHRTN
                     DKQFKDFIKLYFNDISKIPLLTGEEERDIARKIKKGDEEAKKVLIESNLRLVISIAKK
                     YFGSRLSFSDLIQEGNIGLIKAIEKFDPDKEFKFSTYATWWIKQSITKAIADMTKNVR
                     IPVHLIDEINSYNKTYQLLFQKFGREPTSKEIGKELDFPMKKIKKLEEVIFGNVSLDR
                     EIGDEGRDTLADIIEDGNTLRPDQFAEKNALRHNLDTILSMLDDRESKIIKMRYGIDG
                     PRYTLEQVGEEFDVTRERVRQIEQKVIQKLKDHEGLLKILGIEDDIAKIEEMEKMGKR
                     KKGKRVKKIEEDEEEEIDYSYQEYLSDFDDDDDI"
     gene            1946..3841
                     /locus_tag="PK674_02950"
     CDS             1946..3841
                     /locus_tag="PK674_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="DNA gyrase subunit B"
                     /protein_id="HOG15524.1"
                     /translation="MVDNKQYDASNIKVLEGLEPVRQRPGMYIGSTDVVGLHHMIQEI
                     VDNAVDEALAGFCTHITTILHADNSVTVYDNGRGIPVDKHPKTGKSALETVFTMLHAG
                     GKFDKSVYKISGGLHGVGASVVNALSERLEVEVHKDGKIHKIRFERGIPVGEMEIIGE
                     TDKTGTIVKFLPDKKIFDTIEFSGSTEVARMKQSAYLTPGVTFTIINEKESVRQRFYF
                     EGGIKTWLNNLVGEQTRFSSAHYINQEGIDCLAEIAFQFVNTSNDHTLSFVNNIPTKD
                     GGSHVLGFRSALLQAINEVGKEKDKIDKKIGEFQYSDVTDGLYAIVNVKIPEPQFEGQ
                     TKGRLGNAYVRVEVEKIMYEYLINTFRTNEEEFDKIFDKINLAARARLAAKFAKETVL
                     RKNILVGGVLPGKLADCAKKGKEGTELYIVEGDSAGGSAKQGRDSYFQAILPLRGKIL
                     NTEQASIQKTMANAEVKNLITAIGAGIKESFDEENLRYEKVIIMTDADVDGAHIRTLL
                     LTFFFRFMRPLIENGHLYAACPPIYKFKQGKEEKYIYKEEETPEDHGFESDKTEIQRY
                     KGLGEMNSEQLWSTTMDPNNRRLNQITVEDAEEADRLFRILMGEDVMARKHFILTHAK
                     TVKEIDI"
     gene            complement(4061..>4302)
                     /locus_tag="PK674_02955"
     CDS             complement(4061..>4302)
                     /locus_tag="PK674_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15525.1"
                     /translation="NTEYTLTTLDLGVFDFENGVILDVDLDFRHPDMSIENYQKTINI
                     TKKLIKKSRVVTIATSPYFLDQNLALKFLKDILIS"
BASE COUNT         1651 a          543 c          807 g         1301 t
ORIGIN      
        1 acttctattc aaaaggttga attttgaata aaaatttgtt ttcaatattt ttttacttct
       61 caattaatta tgtgtaacat tgtaggaata tgtgtgtaaa gtgtgtaaag tttatccctt
      121 accacccgcc ccgtagtcct acaaggctta caagatgagt ggaacggggt ttcgtaagga
      181 tttcaattag aatccctccc tcgggggagg atgtggtgtc ccgaatttat gagggactag
      241 gtgggggttt tacccccaac taccccgccc cgtaggacta cggggcgggg cgcagcacta
      301 gaacagcttc ccccaaggga agaatttttt cattcttaaa gttttcaact ttacaatagc
      361 taatttttat attttttcaa gtatttttgt gttgatatat gggtaaaaat ttgtataaag
      421 tcttatagat taattaaaaa acattctaat atttctatta gaatgctttt gtaaacttaa
      481 caactgttgc tagagctcca tcacacagtt tatattatat ataaaaaaat gtatttgtca
      541 aatattttgt tttattatta ataatcggct actgtatgtc aaaaattaaa caacaattgc
      601 gagaaaaact tgaagatgat attacagaat taacaaaacc attacaatta ctgttaaata
      661 aatgatttac ctcttgaact gtaaatgaag aggatattat ttccgagatt gatgacatgg
      721 atatgagttc taaattattg gagaaatttt atgaattagc agaaaaatta agtataaaag
      781 ttttgactat cgaagaaatc ttggaaaagg aggcacaagc cttggtagaa aaaagtaagt
      841 taggaaagat agaattgtat gaaactacac atagaactaa cgacaaacaa tttaaagatt
      901 ttataaaact atatttcaac gatatatcca aaatccccct actaacttga gaagaagaaa
      961 gagacatagc tagaaaaatt aaaaaatgag atgaagaagc aaaaaaggta ttgatagaat
     1021 ccaatttaag attagttatt agtattgcta aaaaatattt tggtagtaga ttgagttttt
     1081 ctgatctaat acaggagggg aatatttgat tgataaaggc tattgaaaaa tttgatcctg
     1141 ataaagaatt taaatttagc acttatgcta cttggtggat aaaacaaagt attacaaaag
     1201 ctattgctga tatgacaaaa aatgtaagaa tacctgttca tctaatagat gaaataaaca
     1261 gctacaataa aacatatcaa ttgttatttc aaaaatttgg tagggaacca accagtaaag
     1321 aaatttgaaa agaactggat tttcctatga aaaaaattaa aaagttagag gaagttatat
     1381 tctgaaatgt atcattggat agagagatag gagatgaatg aagggacact ttggctgata
     1441 ttatagaaga tggcaacaca ctcagacctg atcaatttgc agaaaaaaat gcattaagac
     1501 ataatttgga tacgattctt tctatgctgg atgatagaga gtcaaaaatt attaaaatga
     1561 gatatggaat agattgacca agatataccc tagaacaagt atgagaggaa tttgatgtga
     1621 ctagagaaag agtaagacaa atagaacaaa aagttatcca aaaactaaaa gaccatgaat
     1681 gattgctgaa aatactggga attgaagatg atattgccaa aatagaagaa atggaaaaaa
     1741 tgtgaaaaag gaagaaatga aaaagggtca aaaaaataga agaagatgag gaggaagaaa
     1801 tagattattc ttatcaagaa tatttgtccg actttgatga cgatgatgat atttagacca
     1861 aataaggata ataatcacaa aacatatcaa tttacaactt tatatatgat taaaaaatat
     1921 tttttagatt ttaatttatg tgttgatggt agacaataaa caatatgacg cttcaaatat
     1981 taaagttttg gagtgattag aacctgttag acaaagacct ggtatgtata tcggttctac
     2041 tgatgtagta tgattgcatc atatgataca agaaattgtg gataatgctg tagatgaagc
     2101 tttggcatga ttttgtacac atataactac tatattacat gcagacaatt ctgttaccgt
     2161 ttacgataat ggtagatgaa tccctgtaga taaacatcca aaaactggta aatctgccct
     2221 agaaacggtt tttacgatgt tacatgctgg ttgaaaattt gataaatcag tttataaaat
     2281 atctggaggt ttgcatggag ttggagcttc tgttgtaaat gcattatcgg agcgactgga
     2341 agtagaagta cacaaagatg ggaaaatcca taaaataagg tttgaaagat gaataccagt
     2401 atgagaaatg gaaattattt gagaaaccga caaaacttga actattgtaa aatttcttcc
     2461 tgataaaaaa atatttgata cgatagagtt tagtggaagt acagaagttg caaggatgaa
     2521 acaatctgct taccttactc ctggagtaac ttttacaatt atcaatgaaa aagaatctgt
     2581 tagacaaaga ttttattttg aatgaggtat aaaaacttgg ttaaataatt tagtatgaga
     2641 acaaacaaga ttttcctcag ctcattatat aaatcaagaa tgaatagatt gtcttgcaga
     2701 aatagcgttt caatttgtaa atacctctaa tgatcacact ttgtcttttg ttaataatat
     2761 tcctacgaaa gatggaggta gccatgtttt gtgatttaga tctgctcttt tgcaggcaat
     2821 caatgaagta tgaaaagaaa aagataagat tgataaaaaa ataggagagt ttcaatacag
     2881 tgatgtaact gacgggcttt atgctatcgt aaatgtgaaa attccagaac ctcaatttga
     2941 aggtcaaact aaatgaagac ttggaaatgc ttatgtaaga gtagaagtag aaaaaatcat
     3001 gtatgagtat ttgataaata catttagaac aaatgaagag gagtttgaca aaatttttga
     3061 taaaataaat ttggccgcta gagctagatt ggcggctaag tttgcaaaag aaacagtatt
     3121 aagaaagaat atattagtat gaggtgtttt gcctgggaaa ttagcagatt gtgccaaaaa
     3181 atgaaaagaa tgaactgaat tatatatagt ggagtgagat tcagcatgag gaagtgcaaa
     3241 acaaggtcgt gatagctatt tccaagctat tctgccactg agaggtaaaa tattaaatac
     3301 agaacaagct agcatacaaa aaacaatggc caatgctgag gttaaaaatc tgattacagc
     3361 aatttgagcc tgaataaaag aaagttttga tgaagaaaat ttaagatatg aaaaagtaat
     3421 aataatgact gatgccgatg tagattgagc ccatattagg actttattgt taacattttt
     3481 ctttagattt atgaggccgt tgatagaaaa tggtcacttg tatgcggctt gtcctcctat
     3541 ttataaattt aaacaatgaa aagaagaaaa atacatttat aaggaagaag aaactccgga
     3601 ggatcattga tttgaatctg ataaaacaga aatccagaga tataaaggtc tttgagaaat
     3661 gaattcagaa caactttgga gtactacaat ggatccaaac aatagaagat taaatcaaat
     3721 tactgtagaa gatgcggaag aagcggatag attatttaga attttgatgt gagaagatgt
     3781 tatggctaga aaacacttta tactaaccca tgcaaaaaca gtaaaagaaa tagacatata
     3841 atgaagttgg aaaacctact ccccgttcct gtaagttccc cttagaacta cggggctacg
     3901 gaataggatt acggaaagga gttcgtaaag tattaataac tttataaact ttttaacttg
     3961 caactttata aacccccgcc ccgcagcccc gcaatactgc ggggcggggt ggggcggggc
     4021 tacatggcgg ggcaggcttg taacttgcaa cttgtaactt ttaactaatt agtatatctt
     4081 ttaagaattt tagagctaaa ttttggtcta gaaaataggg agaagtagct atcgtcacta
     4141 ctctactctt ttttattaat ttttttgtaa tatttatagt tttttgataa ttttctatac
     4201 tcatatctgg atgtcgaaaa tctagatcta catcaagaat aactcaattt tcaaaatcaa
     4261 aaactcataa atctaatgtc gttaaagtat actcagtatt ca
//
LOCUS       DAOSID010000064         4261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_18306_length_4261_cov_18.666191, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000064 DAOSID010000000
VERSION     DAOSID010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18306_length_4261_cov_18.666191"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..238)
                     /locus_tag="PK674_02960"
     CDS             complement(5..238)
                     /locus_tag="PK674_02960"
                     /inference="COORDINATES: protein motif:HMM:NF019481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HOG15526.1"
                     /translation="MNSIKKGIYKHYKGNLYELIDIVIHSESGEELVLYKALYDDTKG
                     KSQLRVRPKKMFFEDVIIDGIKKPRFEYLGEKI"
     gene            complement(255..971)
                     /locus_tag="PK674_02965"
     CDS             complement(255..971)
                     /locus_tag="PK674_02965"
                     /inference="COORDINATES: protein motif:HMM:NF020733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="N-glycosylase/DNA lyase"
                     /protein_id="HOG15527.1"
                     /translation="MKIESFISKYNIQKIKKLEQKDPQFLALKESWEKIQNKDENLFL
                     YLTLQCALVSYQIAGTGELRRQEFGDKIQKDRKILQELRQKKQDNRVWRYDFLTNSKY
                     NKRIYNIKTNRIKKFNKILQKNIIFQNFGNKLDELNDLISKVMNTKKDSKTNVFAVKM
                     FGYAHEILSGKETIYPMNIQIPVDSRIKRIYTNSLDIKNKVSNTEIQNYFQTLSKKYN
                     IPPLHLDSILWLDYWTKFFI"
     gene            complement(962..1387)
                     /gene="mscL"
                     /locus_tag="PK674_02970"
     CDS             complement(962..1387)
                     /gene="mscL"
                     /locus_tag="PK674_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOG15528.1"
                     /translation="MIKEFKEFAIKGNMIDMAIGIMIGAAFSTIVKSLVDDIIMPVIS
                     GIFKIPDFSNLFVVLSNPSGEVFSSVALAREAGASVLAYGLFINAILAFLMVSVTLFF
                     VVKGINKLKKSEKKVEEPKISELDVLIDIRDQLKKTKGK"
     gene            1680..>4261
                     /gene="ppdK"
                     /locus_tag="PK674_02975"
     CDS             1680..>4261
                     /gene="ppdK"
                     /locus_tag="PK674_02975"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOG15529.1"
                     /translation="MGLFGTKTKKFVYSFNESKGVDKYIIGGKGANLAEMMSLGLPIP
                     DGFTVSTEACEFYYKNNKTNSQEVLEQIEAKLQKLEKTMGKKLGDSQDPLLVSVRSGA
                     AVSMPGMMDTVLNLGLNDESVIGLANRTGNERFAWDSYRRFIQMFGDVVMGVEHHHFE
                     EQLHKVKTEKGIKDDTDLTVEDLQKVVSLYKDMIQRDTGKMFPNDGREQLKMAINAVF
                     DSWNNDRAIVYRKLNNIKGLIGTAVNVQSMVFGNMGETSGTGVCFTRNPSTGENKFYG
                     EFLMNAQGEDVVAGIRTPEHIEELEKVMPECYKELVDIYHLLEKHYKDMQDLEFTIQE
                     GKLYILQTRSGKRTATAAVKMAVDMVDEGLIDEKTAILRINPEQLDALLHKQIEASAK
                     HSAELLTKGLPASPGAGVGQIVFTAEDAHEWAQEGKKLILVRNETSPEDIQGMHASQG
                     VLTARGGMTSHAAVVARGMGKCCVAGASEIVIDEKSKTLTIGSKTFREGDVLTLDGST
                     GEVFAGELKTVDPELSGDFATLMQWADKYRRLGIRTNADTPADALIAKKFGAEGIGLC
                     RTEHMFFADDRIKAVREMILSDNLDDRKKALAKLLPYQKEDFVGLFNAMEGLPVTIRF
                     LDPPLHEFLPTEEHDIVELAKEMNVELSVLKDRIASLHEINPMLGHRGCRLVITFPEI
                     AEMQTEAVISAACELAKAGKQVMPEIMIPLVGFKQELDFNLEIVHRVAKSTMEKYGVE
                     VNYTVGTMIEVPRGALTADKVAESAEFFSFGTNDLTQMGLGFSRDDAGKFIKEYVNQG
                     IFDKDPFQTLDQEGVGQLVEIAVEKGKSTRPNIKLGICGEHGGDPASIEFCHRVGLTY
                     VSCSP"
BASE COUNT         1432 a          564 c          761 g         1504 t
ORIGIN      
        1 tttcttaaat tttttctcat aaatattcaa aacgaggttt tttgattcca tctataatta
       61 catcttcaaa aaacattttt tttggtctga ctcgtaattg agattttcat tttgtatcat
      121 cgtaaagagc tttatacaat accaactctt ctcaactttc actatgtatt acaatatcaa
      181 ttaattcgta caaattgcct ttatagtgtt tgtatattca ttttttaata gagttcatct
      241 tttggaacta attattaaat aaaaaacttt gtccaataat ctaaccaaag aatggaatct
      301 aaatgtagtg gtggaatatt gtattttttt gataaagttt gaaaataatt ttgaatttct
      361 gtattgctaa ccttattttt tatgtctaaa gaattcgtat aaattctttt aatcctgcta
      421 tcaactggta tttgtatatt catgggatat atcgtttctt ttcaagaaag gatttcatgg
      481 gcatatccaa acattttaac ggcaaaaaca ttcgttttgc tgtctttttt tgtgttcatt
      541 accttggaaa taagatcatt taattcatct aatttgtttc aaaaattttg gaaaattata
      601 tttttttgta aaattttgtt gaatttttta attctattag ttttgatatt atagattctt
      661 ttgttgtatt tagaatttgt cagaaaatca tatcgccaaa cacgattatc ttgttttttt
      721 tgtcgtaatt cttgtaatat ttttcggtct ttttgaattt tatctccaaa ttcctgtcgt
      781 cgcaattctc atgttcaggc gatttggtag cttactagag cacattgtag agttaaatat
      841 aaaaacaaat tttcatcttt attttgtatt ttctcccagc tttcttttaa tgctaaaaac
      901 tgtggatctt tttgttccag ttttttaatt ttttggatat tgtattttga tatgaagctt
      961 tctattttca tttagttttt ttcaattgat ctctgatgtc tattagcaca tctagttctg
     1021 atatttttgg ctcttctact tttttttctg attttttgag tttgttgata ccttttacta
     1081 caaaaaataa tgtaacgctt accataagaa acgctaatat tgcgttgata aatagtccgt
     1141 aagctaatac agaagctcaa gcttcacgag ctaatgctac actagaaaaa acttctccac
     1201 tgggatttga taaaactaca aatagatttg aaaaatctgg tattttgaat atcccagata
     1261 taactggcat aattatatca tctactagag attttactat agtagaaaat gctgctcaaa
     1321 tcataattca tatagccatg tctatcatgt ttcactttat ggcaaattct ttgaattctt
     1381 taatcatttt ttattgtatt taaataaaac agttattttt ttagttaaaa atctaaagtt
     1441 ttcaaggata tttttcatta gtttgtaaag ttcataaagt tgcaagtttt tcctaccacc
     1501 cgggtgaaac agaccccggg ataactcccg gggtcaaaac agcacccggg tggtaggaag
     1561 cttttcctca ttgtggactg tgtcagtttt tttgtctata tttgtctact tgctattgaa
     1621 aaacaaaaag aatattttat tattcgtttt gaagttttat tttttttatg taaaaaatta
     1681 tgggattatt ttgaactaaa accaaaaaat ttgtttattc tttcaatgaa tctaaaggag
     1741 tagacaaata cattattgga gggaagtgag ctaatttagc ggaaatgatg tctttggggc
     1801 tacctattcc tgatgggttt actgtaagta cagaggcttg tgagttttat tataagaata
     1861 ataaaaccaa tagtcaggaa gtattggagc agatagaagc taaattacaa aaacttgaaa
     1921 aaactatggg taaaaaatta tgagattcac aagacccatt gttggtttct gttagatctg
     1981 gggcagctgt ttctatgcct ggtatgatgg acacagtatt aaatctatga ctaaatgatg
     2041 aatctgtgat ttgattagcc aacaggactt gaaatgaaag attcgcttgg gatagttata
     2101 gaagatttat tcagatgttt ggagatgttg taatgtgagt agaacaccat cattttgaag
     2161 aacaactaca caaggttaag acagaaaaat gaataaaaga tgacacagat ttaaccgttg
     2221 aagatttaca aaaggtagtt tctttgtaca aagatatgat acaaagagat acttgaaaaa
     2281 tgtttccaaa tgatggtaga gaacagttaa aaatggctat aaatgctgta tttgattctt
     2341 ggaacaacga tagagctatt gtttatagga aattaaacaa tattaaatga cttatatgaa
     2401 ctgctgtgaa tgttcaatca atggtttttg ggaatatggg tgaaacttca tgaacaggag
     2461 tatgttttac aagaaatcct tctactggag aaaataaatt ttatggagaa tttttgatga
     2521 atgctcaggg agaagatgtg gtagcatgaa ttagaactcc tgaacatatt gaggaactag
     2581 aaaaagttat gccagaatgt tataaagagc ttgtagatat ttatcatttg cttgaaaaac
     2641 actacaaaga tatgcaagat ttggaattta ctatccaaga atgaaaactt tatattcttc
     2701 aaacaagaag tggaaagagg acagctactg ctgcagtaaa aatggctgta gatatggttg
     2761 atgaatgatt gatagatgaa aaaacagcta ttcttagaat caatccagaa cagcttgatg
     2821 ctttacttca caaacaaata gaagcttctg caaagcattc agcggagcta ttgacaaaag
     2881 gtcttcctgc ttctcctgga gctggtgtat gacaaattgt gtttacagca gaagatgctc
     2941 atgaatgggc tcaagaaggt aaaaaattaa ttttagttag aaatgagaca tctccagaag
     3001 atattcaatg aatgcatgct tctcaatgag ttttgactgc tagatgaggt atgacttcac
     3061 atgctgcagt agtagctaga tgaatgtgaa aatgttgtgt tgctggagct agtgagattg
     3121 taatagatga aaaatctaaa acactcacaa taggttcaaa aacatttaga gaatgagatg
     3181 ttttgacatt agatggttca actggagaag tttttgcatg agaactaaaa acagtagatc
     3241 cagaactaag ttgagatttt gctacattaa tgcaatgggc agataaatat agaagacttg
     3301 gaattagaac aaatgctgat actccagcag atgctttgat agctaaaaaa tttggagcag
     3361 aaggtatatg actttgtagg acagaacata tgttctttgc tgatgataga attaaagctg
     3421 ttagagagat gattttatct gacaatttgg atgatagaaa aaaggctctt gctaaacttc
     3481 ttccatatca aaaagaagac tttgttggct tattcaatgc tatggaatga cttcctgtta
     3541 cgattagatt tttggatcct ccattacatg agttcttacc aacagaagaa catgacattg
     3601 tagaattagc taaagaaatg aatgtagaac tttctgtact aaaagataga atagcttcac
     3661 ttcatgaaat taatcctatg ttgtgacata gatgatgtag attggttatt actttccctg
     3721 agatagcaga aatgcagact gaggccgtga tttcagcggc ttgtgaatta gctaaagcag
     3781 gaaagcaagt aatgcctgaa attatgattc cattagtatg attcaaacaa gagttagatt
     3841 tcaatctaga aattgtacac agagttgcaa aaagtactat ggaaaaatat ggagttgaag
     3901 taaattatac agttggaaca atgatagaag ttcctagatg agctttgact gctgataaag
     3961 ttgctgaatc tgctgaattc ttttcttttg gaacaaatga tcttactcag atgggattag
     4021 gattttctag agatgatgca ggtaaattca tcaaagaata tgtaaatcaa ggtatttttg
     4081 ataaagatcc tttccaaacc ttagatcagg aatgagtagg acaattagta gaaatagcag
     4141 tagaaaaatg aaaatctact agaccaaata tcaagttagg aatttgtggt gaacatggat
     4201 gagatcctgc ttctattgaa ttctgtcata gagttggact tacttatgtt tcttgttctc
     4261 c
//
LOCUS       DAOSID010000065         4216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_18559_length_4216_cov_10.103100, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000065 DAOSID010000000
VERSION     DAOSID010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18559_length_4216_cov_10.103100"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..3305
                     /locus_tag="PK674_02980"
     CDS             87..3305
                     /locus_tag="PK674_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOG15530.1"
                     /translation="MEKITHLFVHRKDTKHYFHRHGKKYLFGTLGAFAVVKMVLLFVW
                     FFSTLDFGMSYAQTEGLLSKETIISMGCPTDAITCELSNRSITTIESDAFVNHTQLQV
                     LNLSFNQLTGLDGVEFPSLLGNLSLISNQIASLDGVEFPDGLQVLNLSFNSLTSISGV
                     EFPDSLVYVKFSNNQLTNLDGIALPSSVSYLDFSFNQITGISGLVLPSSLKELYLNEN
                     QLTSLDGLEFSDPLSILYLAGNQITNISGIEFPDSLLVLNLAENQLTSLDGVEFPDSL
                     RELFFYDNQITSLSGVVLPSSLDVFYFFNNQLTSIDGIEFPSSLKILLIHRNKLTSVP
                     ESIMQLANLSDNGGLNVDYNYIEFDQLSPSLLAFIDQKSEGNWRSTQQTEAGQVAVGG
                     CTDVNANNYNPDATYDNGSCLYGEVGLPSNIGDWSIDGMFLMTQILPDAKIYSGGLLP
                     FLIAGEYSEANGVAGGEVHVGVPLNFEYQAIEFGSNRTGGNIVYGKPGDRCYADFIQG
                     TGKYVEYIDTLLEETILPQVLECDNSPELSCQLNIPELSPDPIPLSHSAEIYSTTGMK
                     AWVWAIVDVFNLLGIMLENDGFIGISNMAEQALLDGVPPSTIISQVSIFVSNSYDINT
                     YPVFEIILPSLGGNLGLETVETCGNKVYSTNISKKPDSSASNELYFFGKHGYVPEGFD
                     IYLFVSSEGIIDTDISNNMSTIHIDLAEGEGMNCANRSETAIVADGNIGEIFINSGGC
                     QSELAWCSDGDLYLIGDGLLPIGDQEFYANNICMHGPRVSLVSIVGETLEGSVLTGEY
                     IYSGEIKEGLSEYQWYRNNIPIDGAKQITYIVSGEDLGKTLYFEVTPLDEDGKIRGKT
                     VMSAGLYIPDPNNPIGENRPNIGGFSSAAILVEPEQVQNLAMNLNINNQSNELVRAYA
                     YSYLKGITTMPTMAEADMHGPLSRIALAKMISKYVLSEGLQEIDTNKKCDFPDVPDDL
                     NKDYDDGVTKACQLGLMGVGIEEFNPEGIVVRAEFGTVLSRALWGDKYNSDGVYYEKH
                     LEALKKEGIMTKIDKPFDTEQRGWTMLTFLRAGKMLLKNKSH"
     gene            complement(3726..4187)
                     /locus_tag="PK674_02985"
     CDS             complement(3726..4187)
                     /locus_tag="PK674_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOG15531.1"
                     /translation="MLYGTGLRVSELIQIKRSQINPDNKQFSVVGKGSKIRASFLTKK
                     AKEALVDYLQARTDDSDHLFISLSGNSRGEALSRNSIEEIVRNYANLVGIEKKVTPHT
                     LRHSFATTLLKRGADIRAVQTLLGHSSITTTQIYTHVDDKYLKEVHGLLDS"
BASE COUNT         1426 a          567 c          797 g         1426 t
ORIGIN      
        1 ttagtatgat actaacgttt ttcttttaaa tatttttttt cccggacact actgaaaaac
       61 tttttatatt cttacattaa taaaaaatgg aaaagattac tcacttattt gtgcacagaa
      121 aagatactaa gcattatttc catagacatg gaaaaaagta tttgttcgga acattatgag
      181 catttgctgt agtaaagatg gtgttattat ttgtttggtt ttttagcaca ctagattttg
      241 gtatgagtta tgcccaaaca gaaggactgt tgagcaaaga aactataata tcaatggggt
      301 gtcctactga tgctattacc tgtgaactgt ctaataggtc gattaccact atagaaagtg
      361 atgcctttgt aaatcataca cagttgcagg ttttaaatct ttcgtttaat caactaacgg
      421 gcctagactg agtagaattc cctagtttgt taggaaattt aagtcttata agtaaccaaa
      481 tagcaagcct agactgagta gaattccctg atggcttgca ggttttaaat ctttcgttta
      541 attcactgac aagtatatct tgagtagaat tccctgattc tttggtttat gtaaaatttt
      601 ccaataacca attaacaaat ctagattgaa tagcgttacc tagttctgta tcgtatttgg
      661 atttttcttt taaccaaata acaggtatat cttgactagt acttcctagt tcgttaaaag
      721 agttgtatct taatgagaac caactaacaa gtttggattg actagaattt tctgaccctt
      781 tatcgatttt atatcttgct ggaaatcaaa taacaaatat atcttgaata gaattccctg
      841 actctttatt ggttttaaat cttgctgaaa atcaactaac aagtttagat tgagtggaat
      901 tccctgattc tttgagagaa ttattttttt atgacaacca aataacaagt ctatcttgag
      961 ttgtattacc tagttcattg gatgttttct atttttttaa taatcaacta acaagcatag
     1021 actgaataga atttcctagt tctttgaaaa ttttacttat tcataggaac aagctaactt
     1081 cagtaccaga aagtattatg caattggcaa acttatcaga caattgatga ttaaatgttg
     1141 attataatta tatagaattt gatcaattat ctccaagtct tttggctttc atagatcaaa
     1201 aatctgaggg gaattggaga tctactcaac aaactgaagc aggacaagta gctgttgggt
     1261 gatgtacaga tgttaatgcg aacaactata atccagatgc gacttatgat aactgatctt
     1321 gtttgtatgg agaagtaggt ttaccaagta atatatgaga ttggtcaatt gacggaatgt
     1381 ttttgatgac tcagatatta cctgatgcaa aaatatatag tggaggttta ttaccatttc
     1441 ttatagcatg agaatattct gaagctaatg gagtagcatg aggggaagta catgtgtgag
     1501 tgcctttaaa ttttgagtac caagcaatag agtttggttc aaatcgaact ggttgaaata
     1561 tagtttatgg aaaacctgga gatagatgtt atgctgattt tatacaatga acaggaaaat
     1621 atgttgaata catagacact ttattagaag aaacaattct tccacaagta ttagagtgtg
     1681 ataattctcc tgagctaagt tgtcaattaa atattcctga attatctcct gatccaatac
     1741 ccttgtctca ttcggcagaa atatattcca ctactggtat gaaagcttgg gtgtgggcga
     1801 tagtagatgt ctttaattta ttatgaatta tgcttgagaa cgatggtttt atagggattt
     1861 caaatatggc agaacaggct ttattggatg gagtaccacc ttctactatt atatcacaag
     1921 tatctatatt tgtatccaat agttatgata taaatactta tccagtattc gagattatat
     1981 taccttcgct tggttgaaat ttatgattgg aaacggtaga aacttgtggg aataaggtat
     2041 atagtacaaa tatttcgaaa aaacctgatt catctgcatc taatgaactt tatttttttg
     2101 gtaagcatgg ctatgtacca gaatgatttg atatatattt atttgtaagt tctgagggaa
     2161 taatagatac tgatatatct aataatatga gcacaataca tatagatcta gcagagtgag
     2221 aatgaatgaa ttgtgctaac agatcagaaa ccgctattgt agctgatgga aatataggag
     2281 agatttttat caatagctga tgatgtcaga gtgaattagc ttggtgttcg gatggggatc
     2341 tgtatttaat atgagactga ttattaccta tatgagatca agagttttat gcaaacaata
     2401 tttgtatgca tggtccgaga gtaagcttag tttcaatagt atgagaaaca ttggagtgaa
     2461 gtgttttgac atgagaatat atatattctt gagaaataaa agaatgatta tcggaatatc
     2521 aatggtatag aaacaatatt cctattgatt gagcgaaaca aataacttac atagtaagtg
     2581 gagaagattt atgaaaaacc ttatattttg aagtaactcc attagatgag gatggaaaaa
     2641 taagatgaaa aacagtaatg agtgctgggc tttatattcc agatccaaac aatcctattt
     2701 gagaaaatag accaaacatt tgaggttttt caagtgctgc tatactagta gaaccagaac
     2761 aagtccaaaa tttagctatg aatttgaata tcaataacca atctaatgaa ttagtaagag
     2821 cttatgctta ttcatattta aaatgaatta ccacaatgcc aacaatggcg gaagctgata
     2881 tgcacggtcc attgagcaga atagctttag ctaagatgat atccaagtat gtgttaagtg
     2941 aaggtttgca agagatagat acaaataaaa aatgtgattt cccagatgtg cctgatgact
     3001 taaataagga ttatgatgat ggagtgacaa aggcatgtca actaggactt atgggagttt
     3061 gaattgaaga atttaatcca gaaggaatag tagttagggc ggaatttggt acagtattgt
     3121 ccagagcttt atggtgagat aagtataata gtgatggagt atattatgaa aagcacttgg
     3181 aagcattaaa gaaagagtga ataatgacaa aaatagataa gccgtttgat acagaacaaa
     3241 gatgatggac gatgttgact tttttgagag cgtgaaaaat gttgttgaaa aataaaagtc
     3301 attaatctaa aagaaatagg ggcgaatttt cgttcgcgac ggagtgataa attgttcgca
     3361 tcgcttaagc ggtgtgaact ttgctcacga taaaatgata aagtgatgaa accccgctta
     3421 gcggggtgaa tgataaaatg atgaaatgtt taaatgataa aataaatatt cttgatttct
     3481 ggcaggcgcg aattcgaatt cgcgcctaca aagatttggg tgttagcact tcctgaatac
     3541 aaatttcgtt cgcaataaaa tgatgaatgc tcacggtgtt cgcgataaaa tgatgaatgc
     3601 tcacagtgtt cgcgataaaa tgttaaaatg ataaagtgat aaagtgatga aaccccgctt
     3661 agcggggtga atgataaagt gataaagtaa tgaaatattg aactatattt ttaatctctt
     3721 tttttttaag aatctaataa accatggact tcttttaaat atttgtcatc tacatgagta
     3781 tatatttggg tagttgtaat actggaatgt cctaataaag tttgaacagc tctaatatct
     3841 gctcatcttt tgagtagtgt ggtagcaaaa ctatgacgaa gtgtatgggg agttaccttc
     3901 ttttctattc atactaaatt ggcataattt ctaacaattt cttctatcga atttctgctt
     3961 aaagcttctc atctactatt tccggataaa cttataaata aatgatctga atcatctgtt
     4021 ctagcttgta gataatctac taatgcttct tttgcttttt ttgtaagaaa gcttgctcta
     4081 attttggatc attttcatac tactgaaaac tgtttattgt ctggatttat ttgggatctt
     4141 ttgatttgaa ttagttctga aacacgcaga cctgtaccat aaagcatcca aagtatcgca
     4201 gaatctcttt ttcttt
//
LOCUS       DAOSID010000066         4205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_18623_length_4205_cov_14.946265, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000066 DAOSID010000000
VERSION     DAOSID010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18623_length_4205_cov_14.946265"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..>1178
                     /locus_tag="PK674_02990"
     CDS             271..>1178
                     /locus_tag="PK674_02990"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="DNA-processing protein DprA"
                     /protein_id="HOG15532.1"
                     /translation="MSIHPEIIKYSAHPDITFGDLKSGDFDKYDIPASELKNIQSIFQ
                     EHNIGAFMMGQDDYHYKFHSIPSKPYIVHYIGDLSLLDKNILAIVGPRKPSVYSHKVL
                     KKLFEHAENYDLVTISGMAAGVDQLAHALSIKHKIPTIAVLGGGLNYFLKGAERHIVQ
                     SIVDNGGLVLSEYKINFVPTKYSFPQRNRIIAGLSDMVVLPEAGENSGSLITVDFANH
                     SSRPVYVVPNDIFSLTSFGVHQLISQQKANVLIDFKDFFDQNFKKFGVKHTVSTKILD
                     LPAPQSDIIGLLSDNGEMSLGAIMQAT"
     assembly_gap    1179..1278
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1279..3428
                     /locus_tag="PK674_02995"
     CDS             <1279..3428
                     /locus_tag="PK674_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=25
                     /product="DNA translocase FtsK"
                     /protein_id="HOG15533.1"
                     /translation="KNILARRKYKRRKKTVILSKYKLGAGMIILGGLYFFSLYLAEGS
                     PIFELLTNYASVAFGEIGLNVFFGLCILVGILILYKGYLMNLLIKQFLIMMMLVSGVL
                     NFPIIDGEISKYQSFGGYLSWPLIELLNLMFGSQATAVKSFIVVLLIATLVWILYTFN
                     FSLPKINFKLEKYAKPKAAGATRELKIERQTTDDIAKKINETRSGINRPISDSFIKTI
                     IKQKVEEKIEEKRPRPVINFSGDKPTFNTSILKSNTGEVTTIDEKFLMEKAKSLQDKL
                     MEFNVAVSIEGFDIGPTIVQIRIKPEAGIAISKIESLVNDIKLSLKSKSLRIIAPIPG
                     TDSVGIQIPNQKPTIVRVGDMLSSKEFQSQMAKSNTNLTLGRQIDGSIITKTLEDMPH
                     LLIAGATGSGKSVGVNDFIISLMYQNSPSELKFLMVDPKQVELELYSGLPYMLAPIVS
                     EPEQALKLLKRAVDEMEKRYGMLKDRRVKNIGEYNQKVMGDKMYRIVFVIDELADMMM
                     HKNKKEVETYITRIAQKARAIGIHLILATQRPSVNVITGLIKANMPTRIAFGVVSEID
                     SRTILGRKGAEDLVGRGDMLYMDPSTKRPIRIQAPFVETSEIELVVNTLKDKYMRGLS
                     EDDVYNPDIIKALESKLETAGGSFSGGVGGSDEELVEQAIQVILETRKASATLLQRKL
                     NIGFARAARIMDELEERGVIGPQEGAKARDIFI"
     gene            3445..>4205
                     /locus_tag="PK674_03000"
     CDS             3445..>4205
                     /locus_tag="PK674_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="M15 family metallopeptidase"
                     /protein_id="HOG15534.1"
                     /translation="MSFIKNKITSFGNIFIFVCVFVFLFIVRQNSAKADIIRKGGFER
                     VDSVSEFVDNKVVSIFGEALAFSQNDYKQNFDLNSDCSLQKYISDDLVLENIKYVPAD
                     LSQIDSEYIVNRAGRPYLRLSAQTAFEAMAKDFYSANNQQLYLMSAYRSYNDQATLFE
                     GGCSLNRCARLGASEHQLGLAVDIHLATKNGYNILGGEYLERLNQNAHKYGFINTYRK
                     GAKIDGKMAEVWHRRYVGKYLATELFEKDLSFAEY"
BASE COUNT         1416 a          560 c          752 g         1377 t
ORIGIN      
        1 atgagggata attaacaact aataattttg ttccttatta atgggaattg ggagagttgg
       61 aggttgtagt tgggggtaaa acccccacct agtccctcat aaattcggga cattcaacct
      121 ccccctaggg aggaattttc ccctcatcac ttcaacattt tgtcacgaat gttttttact
      181 gtgataacgg gcggttgata actgccccta catcaattca tctctttatc attttaacac
      241 tttatcattt tatcacttta acattttctc atgtctattc atcctgaaat tattaagtat
      301 tctgctcatc ctgatattac tttttgagat ttgaaatctt gagattttga taagtatgat
      361 attccagcat ctgaactaaa aaatatacaa agtatttttc aagagcacaa tatcggagct
      421 tttatgatgt gacaagatga ttatcattac aagtttcatt caataccatc caaaccatat
      481 attgtccatt acatatgaga tttatcttta ttggacaaaa atattttggc gatagttggg
      541 cctagaaagc cgagtgttta ttctcataaa gttttaaaga agctttttga acatgctgaa
      601 aactatgatt tggtaactat ttccggcatg gcagcttgag tagatcagct ggctcatgcg
      661 ctttctatca agcacaaaat acccaccatt gctgttttgt gatgatgact aaattatttt
      721 ttgaaatgag cagagcgtca tattgtccaa tctatagtgg acaatggatg actggtattg
      781 tccgaataca agataaattt cgtacctaca aaatatagtt ttccgcaaag aaatcgtatt
      841 atagcaggtt tgtctgatat ggttgttttg ccggaagctt gagaaaattc ttgaagttta
      901 attacagttg attttgccaa ccattcaagt agaccggtat atgttgttcc aaacgatatt
      961 ttttctctta ctagttttgg agtgcatcag ctaatttctc aacaaaaagc gaatgtctta
     1021 attgatttca aggatttctt tgatcaaaat ttcaaaaaat ttggggtaaa acataccgtt
     1081 agtacaaaaa tattggattt accagctcct caaagtgata ttatttgact tttgtctgac
     1141 aatggcgaaa tgtctttgtg agctattatg caagctacnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnaa aaaaatattt tggctagaag aaaatataaa agacgcaaaa
     1321 aaacagtaat tttgagtaaa tataagctat gagctggtat gattattctt gggggtttgt
     1381 attttttttc tttgtattta gctgaatgat caccaatatt tgaacttttg accaattatg
     1441 ctagtgtggc tttttgagaa atatgattaa atgtgttttt tggattgtgt attttagtat
     1501 gaattttgat cttgtacaaa ggttatctga tgaacttact catcaaacaa tttttgatta
     1561 tgatgatgtt agtttctgga gtattaaact ttccaataat agattgagaa atttctaaat
     1621 atcaatcttt tggtggatac ttatcttggc ctcttattga gcttttaaac ttaatgtttg
     1681 gttctcaagc tacggcagta aaatctttta tagtagtttt gcttatagct actcttgtgt
     1741 ggattttata tacattcaat ttttctttac caaaaattaa ttttaaattg gaaaaatatg
     1801 ctaaaccaaa agcagcttga gctactagag agttaaaaat agaaagacaa acaacggatg
     1861 atattgctaa aaaaataaat gagaccagaa gcggaataaa tagaccaatt tctgattctt
     1921 ttatcaaaac tattataaaa caaaaagtag aagaaaaaat cgaagaaaaa agacctagac
     1981 cagtcataaa tttttcttga gataagccga catttaatac atctatttta aaatctaata
     2041 catgagaagt aactacgatt gatgaaaaat ttttgatgga gaaggcaaaa tcattgcaag
     2101 ataagttgat ggaattcaat gttgctgtga gtattgaatg atttgatata gggccaacta
     2161 tcgttcaaat cagaattaaa ccggaagctg ggatagctat atcaaaaatt gaatcattgg
     2221 taaacgatat taaactgtca ttaaaatcaa aatctttgag aattattgca cctatcccgt
     2281 gaacagattc tgtctgaatt caaattccaa atcaaaaacc gacaatagtt agggtatgag
     2341 acatgttgag ttcaaaagaa ttccagtctc aaatggcaaa atccaataca aacttaacct
     2401 tggggagaca aatagatgga tcaattatta caaaaacttt ggaagatatg cctcatttac
     2461 ttattgcttg agctacttga tctggtaaat ctgtatgagt aaatgacttt attatatctt
     2521 tgatgtatca aaattctcca tcagaattaa agtttttgat ggtagatcca aaacaagttg
     2581 agcttgaact atattcttga ctgccttaca tgttagctcc aatagttagt gagccagaac
     2641 aggcattaaa attattaaaa cgagctgtag atgagatgga aaagagatat ggtatgctca
     2701 aagatagaag agtaaaaaat ataggagaat acaatcaaaa agtaatgtga gataaaatgt
     2761 atcgtatagt atttgtgata gatgagttgg cagatatgat gatgcacaaa aacaaaaaag
     2821 aagtagagac ctacattact cgtatagctc aaaaggctcg tgctattgga attcatttaa
     2881 ttttggcgac acaaagacca tctgtaaatg tgatcacctg acttatcaaa gcaaatatgc
     2941 ctactagaat agcatttgga gttgtttcag aaattgattc taggactatt ttgggtagaa
     3001 aatgagctga ggatttggta tgaagatgag atatgcttta tatggatcca agtacaaaac
     3061 gacctattag aatacaagct ccatttgtag aaacatcaga gatagaattg gtagtaaata
     3121 ctttaaaaga caaatatatg agatgattat cagaggatga tgtatacaat ccagatatta
     3181 tcaaagcttt ggaaagtaaa ttggagaccg cttgatgatc gttttctgga tgagtgtgat
     3241 gatctgatga agagcttgta gaacaagcta tccaagttat tctagaaact agaaaagctt
     3301 ctgctacttt attgcaaaga aagcttaata taggttttgc tagagcggct agaattatgg
     3361 atgaacttga agaaagaggt gttatttgac cacaagaatg agcaaaagct agggatatat
     3421 ttatataaaa tttagatcta aaaaatgagt tttataaaaa ataagatcac ttcgttttga
     3481 aatatattta tttttgtttg tgtttttgtt tttcttttta ttgttcggca aaattcagca
     3541 aaagcagaca tcattagaaa aggtggtttt gaaagagtag atagtgtgtc agaatttgta
     3601 gataataaag tggtttctat ttttggtgaa gccttggcct tttcacaaaa tgactacaaa
     3661 caaaactttg atcttaattc agattgttct ttgcaaaaat atatttcaga tgatttggtt
     3721 ttagagaata taaagtatgt cccagctgat ttatcgcaaa tagattctga atatattgta
     3781 aatagagcgg gtagacctta tctgagattg tctgctcaaa ctgcttttga agctatggca
     3841 aaagattttt attcagccaa taatcagcaa ctttatttga tgagtgctta tagatcctac
     3901 aatgatcaag ctactttgtt tgaatgaggt tgttctttga atagatgtgc tagattgtga
     3961 gctagtgagc atcagctttg attggcggta gatatccatt tggcgaccaa aaactgatac
     4021 aatattttat gaggagaata tttggagcga ttaaatcaaa atgcacataa atattgattt
     4081 atcaacacat atcgtaaatg agctaaaata gatgggaaaa tggcggaagt ttggcatcga
     4141 agatatgttt gaaaatactt ggctacagaa ttgtttgaaa aggatttaag ttttgcggag
     4201 tatta
//
LOCUS       DAOSID010000067         4025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_19707_length_4025_cov_16.428212, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000067 DAOSID010000000
VERSION     DAOSID010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19707_length_4025_cov_16.428212"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PK674_03005"
     CDS             complement(<1..875)
                     /locus_tag="PK674_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOG15535.1"
                     /translation="MSTHRLVIKDAIRQLIGRVISGLAGFLVVKFMSPYLGPLRYGDY
                     STILKYFAIWSAMADFGLYVIAVRQLGQIKSKEKNSIEKPELKSMYGKFVGARFSIMF
                     VVYFVAIIVAYFLPAYTSNPYIVRGLPLGMIFSASFMAAGILQLPLQLFWRMKDLSVG
                     LILARVSQIILLIITIFVLLPSVDFTSGDPKSIIAFCLILLSVVVSGLTQIFYVLYKS
                     NKKLKLKVNFDKKFTLGIIKSNWKYGLSFYLSSFHTLIVLIFLSNFFPTAKGYVFTGI
                     WGLGLTLIEIFLIIPS"
     gene            complement(972..1427)
                     /locus_tag="PK674_03010"
     CDS             complement(972..1427)
                     /locus_tag="PK674_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15536.1"
                     /translation="MELKVFNGEKFERDKVRYIVFASIFILIFVVSAFYKNIVGIILM
                     FFLLGAYIYYGIINVNEITITISENGLLVGTKVIPWTNLVGYCIELELKKQQIKNIVF
                     VSQRGHTIYTINDSEENIKTFLENLNQMLPMLTDFPQNFWEKIVRKMKL"
     gene            1770..>2603
                     /locus_tag="PK674_03015"
     CDS             1770..>2603
                     /locus_tag="PK674_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG15537.1"
                     /translation="MDFVEIGKKWQKFWKENQTYKVVEKKDKKSFYILDMFPYPSGAG
                     LHVGHPKGYIATDVIARKHMLQGEMVLHPMGFDTFGLGTEQFAIANKIKPQIAAEQNI
                     ATYKKQLEMFGCTYDWDREVNTADPHYFKWTQKVFLDLYNSYFDEKTQSAKPITDLES
                     KIENGQLKIPVGMTKEQFLDTQRLAYIDYKPINRCPSCKTGLANEDLNSDGTCERCSS
                     KVEQKPMKQRVIRITKYAERLLDGLESLPEWTDSAKEQQRNWIGKSEGTQFKMEVITP
                     SQ"
     assembly_gap    2604..2703
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2704..>4025
                     /locus_tag="PK674_03020"
     CDS             <2704..>4025
                     /locus_tag="PK674_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG15538.1"
                     /translation="TEKGILVNSGQFSGLTSDEAITKMQERLEEKQIGGKKINYRLQD
                     RVFSRQRYRGEPFPIVFCNHCDSKLTINFRNKDTRNGILSGNKTIETRALNPEEPERF
                     FGNIKAGDIVVAINKETNEELRLKIIKTYQRKDLRDMRENEDHETIKKIYRNKEMFEN
                     TKTFDDFESKRALTEGYIEKIKKNGLVGREFELIGPAIVTMKESDLPLLLPDVENYEP
                     TGTEEGPLANIEERINVECPKCGGKAKRESNTMPGWAGSSRYWIRYMDPHNDKELVSS
                     EKEKFWHPVDIYVGGAEHITRHMIYARFWHKFLQDLGIVSVDEPFKKYQHVGLIMAED
                     GRKMSKRRGNVINPDDIINEFGADVLRMYEMFMGPFDQSVNRNTNGVKGIKKFLDKVI
                     SLRSKVGSGNLDDKDIINLLHKTIKKVSEDIDVFGFNTAISQLMIFVN"
BASE COUNT         1489 a          497 c          636 g         1303 t
ORIGIN      
        1 gatggtataa tcaaaaagat ttcaattaaa gttagtccca atcaccatat tcaagtgaaa
       61 acataacctt ttgcagtagg gaaaaagttt gataagaata ttaaaactat gagagtatga
      121 aaactactaa ggtaaaaaga taatcagtat ttccaattcg attttataat tcatagggta
      181 aattttttat caaaattcac ttttagtttt agttttttgt ttgatttgta tagtacatag
      241 aaaatttggg ttaatcagct cactacgaca gataataaga ttaagcagaa agctataatg
      301 gatttgggat ctcagcttgt aaaatctaca ctaggtaata aaacaaaaat agtaattatt
      361 aataaaatta tttgactaac cctagctaga attaacccga cacttaagtc tttcatcctc
      421 caaaacaact gtagaggtaa ttgtagtatt cctgcagcca taaaacttgc tgaaaaaatc
      481 attcatagcg gtagtcatcg aactatgtat gggttactag tatatgctgg tagaaagtat
      541 gctactatga tagccacaaa ataaacaaca aacattattg aaaatcttgc tcacacaaac
      601 ttaccgtaca tagattttaa ttcaggtttt tctatgctat ttttttcttt gcttttaatt
      661 tgtccgagtt gtctaactgc tattacatat aatccaaaat ctgccattgc agaccagatt
      721 gcaaaatatt ttagtattgt actataatct ccatatctca aaggtcccag ataaggagac
      781 ataaatttta ctactaaaaa accagcaagt cagctaatga ctcttcatat caattgtcgt
      841 atagcatctt ttattactaa tctatgtgta gacaaagcgt ttaaatgtta aaatattaaa
      901 gttttgaaat ggtgtaatga aaaaatggag agttgttgaa catctataat aatacattat
      961 ttgtttattc tttataattt catttttcta actatttttt cccaaaaatt ttgcgggaag
     1021 tctgttaaca ttggtagcat ttgattcaaa ttttctagaa aagtttttat attttcttca
     1081 ctatcgttga ttgtgtatat agtgtgtcat ctttggctaa cgaagacaat gtttttgatt
     1141 tgttgttttt ttaattctag ttctatacaa tatcagacta gattagtcca aggaataact
     1201 tttgttcaaa ccaataatca attttctgaa atggtaatag ttatttcgtt tacgtttata
     1261 ataccgtaat atatgtatgc tcccaacaaa aaaaacatta aaataattca aacaatattt
     1321 ttataaaaag cggacactac aaatattagt ataaatatgg aagcaaacac aatgtatcga
     1381 accttatctc tttcaaactt ttctcaatta aatactttta gttccatttt ttggtatatt
     1441 aagtaaagct tatttgaata atacttaagt gaatcgttta ttcaagaaaa aagtcgactt
     1501 tatttagccg acttttcctg aacccatcat cataggtctt tttgttatag aaacttgatt
     1561 tttttttgtt tttttgtttt tgtttatagt tctattatat ttaaaaatac tttttttgtc
     1621 aaaaatttga tgttaaagtt aatatatttg tttttaatga ataaaaagac cctttataac
     1681 attttttctt gtttttaatt aagaaaacac taacttatta atattgtata aattaaggga
     1741 ttatttttta agttttatta taaaaaatca tggattttgt agagattgga aaaaaatggc
     1801 agaaattttg gaaagaaaat caaacttata aagtagtgga aaaaaaagac aaaaaaagtt
     1861 tttatatttt ggatatgttt ccttatccga gtggagctgg attacatgta tgacatccaa
     1921 aatgatatat tgccactgat gtaatagctc gtaagcatat gttgcaatga gagatggtct
     1981 tacatcctat gggttttgat acttttggat taggtacaga acaatttgca attgcaaata
     2041 aaataaaacc acaaatagct gcagaacaaa atattgcaac ttacaaaaaa cagcttgaga
     2101 tgtttggttg tacttatgat tgggatagag aggtcaatac ggcagatcct cattatttta
     2161 agtggacaca aaaagtgttt ttggatttgt ataattctta ttttgatgaa aaaactcaat
     2221 ctgcaaaacc tattacagac ttggaatcaa aaatcgaaaa tggacaattg aagatccctg
     2281 tgtgaatgac taaagaacaa tttttggata ctcaaagatt agcatatatt gactataaac
     2341 caatcaatcg atgtccgagt tgtaaaacat gattggcaaa cgaagattta aattcagatt
     2401 gaacttgtga aagatgtagc tcaaaagttg aacaaaaacc gatgaaacag cgagttatta
     2461 gaattacaaa atatgcagaa agacttttgg attgattgga aagtttacca gaatggactg
     2521 attcagctaa agaacagcaa agaaattgga tatgaaaaag tgaatgaaca caattcaaaa
     2581 tggaggtaat aaccccatcc caannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnacagaaa aaggaattct tgtaaattca gggcaatttt caggacttac ttctgatgaa
     2761 gcaattacta aaatgcaaga acgacttgaa gaaaaacaaa tatgatgaaa aaaaataaat
     2821 tatagacttc aagatcgagt tttttctaga caaagatatc gatgagaacc atttcctata
     2881 gtgttttgta atcattgtga tagtaaatta actattaatt ttagaaataa agatactcga
     2941 aatggtattc tctctggaaa caaaaccatt gaaacaagag ctctcaatcc tgaagaacca
     3001 gaaagatttt ttggtaatat aaaagcatga gatattgttg tagcaataaa caaagaaacc
     3061 aatgaagaat taagactaaa aattataaaa acttatcaac gaaaagatct tagggatatg
     3121 cgagaaaacg aggatcatga aacaatcaaa aaaatatacc gcaataaaga aatgtttgaa
     3181 aatactaaaa catttgatga ttttgaatca aaacgagctc ttactgaatg atatattgaa
     3241 aaaattaaaa aaaatggtct agtatgacga gaatttgaac ttataggacc agctattgtg
     3301 actatgaagg aatcagattt accacttctg ttaccagatg tagaaaatta tgagcctact
     3361 ggtacagaag aaggaccgtt agcaaatatt gaggagcgaa ttaatgtaga atgtccaaaa
     3421 tgttgatgaa aggcaaaaag agaatcaaac actatgcctg gttgggctgg atcatctcga
     3481 tactggatta gatatatgga tcctcataac gataaagaat tggtgtctag tgaaaaagaa
     3541 aagttttggc atcctgtgga tatatatgtc tgaggagctg aacatattac gagacatatg
     3601 atatatgcta gattttggca taagttttta caagatttat gaattgtttc tgtagatgag
     3661 cctttcaaaa aatatcagca tgtttgactt ataatggctg aagatgggcg taaaatgtcc
     3721 aaaagacgat gaaatgtgat caatccagat gatattataa atgaatttgg ggcggatgtt
     3781 ctcagaatgt atgaaatgtt tatgtgacct tttgatcaat ctgtaaatcg aaatacaaat
     3841 ggggtaaagt gaataaaaaa gtttttagat aaagtaatta gtttaagatc aaaagttgga
     3901 agttgaaatt tagatgacaa ggatattata aacttattac ataaaactat taaaaaagtt
     3961 tctgaagata tagatgtttt tggttttaat acagcaatat ctcagttgat gatttttgta
     4021 aatga
//
LOCUS       DAOSID010000068         3966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_20087_length_3966_cov_10.936845, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000068 DAOSID010000000
VERSION     DAOSID010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20087_length_3966_cov_10.936845"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2639
                     /locus_tag="PK674_03025"
     CDS             <1..2639
                     /locus_tag="PK674_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=25
                     /product="DNA gyrase subunit A"
                     /protein_id="HOG15539.1"
                     /translation="PYPLEKDLTQSYIDYAMSVIISRALPDTRDGCKPVIRRILYGMY
                     DMKMFYNTKHKKSARIVGDVMGKYHPHGDSSIYEAMVRLAQPWSMRYPLVDGQGNFGS
                     MDGDGAAAMRYTEARLTKIAEEMINDIEQDTVDRRNNFDGSLQEPVMLPTKFPNHLCN
                     GTMGIAVGMATNLAPHNLNEVIDACLLLIQKEGKEVEVPIDFIDNNENLSQDEGDLQS
                     SDENITENTQNEELPNIDSTTSPDSTNSNGTKTIKYSVSIDEIMEIVKGPDFPTGGYI
                     FDPNRIKEVYSKGRGGIVCRGRHEIEEIKSGKNIVITEIPYMVNKSTLVSKIGELVVD
                     KKIDGIVDIRDESNKNKIRVVLQLRKGINPDQILIQLYKATDLQSNFNVNNVSLVDNG
                     LQPRTLNIKELLMEFVTFRRQVVYRRSNYQLDKARDRLHILEGLKKAIDIIDEVIETI
                     KKSATKQEAKENLMAKFDFSDQQAEYILLMRLQSLVGLEIQKISDEIDEKIKLIEYLE
                     SIINNSEKLDEVVVEELNYIKEKYGDERKTEVSNDLGVYSLGSSLKAYRDAADKIKED
                     VILRIGNDYHVRVLYQSRILAIPEETLELIYTHNQDNLIVITDLGELVVERLKDLGQF
                     TMVKNALDLKEQYGLKGNIIFAKTLHYHYEHLLFLTKDNNIKKINKDLILKFKKFPTI
                     VMKLEAKEKIAAVLAVSDGDNVAIVTEQGRGLLFPASDIRAMGKTAGGVKAIDLQEGD
                     RVSNMFLHKEQPFLLVHNKKEAKLLSLEDLRVRKRARKGDVWATGDCKLEGGISIEEG
                     AIRIRFTDGSLKTLHSNDVKLDMPDTPLKEIVKKDIELIYRPWEEKEENFKGKEERKA
                     REKAEREAEEKRNALFSDGDIE"
     gene            2691..3785
                     /locus_tag="PK674_03030"
     CDS             2691..3785
                     /locus_tag="PK674_03030"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="S1C family serine protease"
                     /protein_id="HOG15540.1"
                     /translation="MGKNKIGSIILTMLMSVITLLVLLLMIKISKLETKISIITGEDI
                     VDITQHQKDFDFKLFEESILKNLGELDKNIVAIYAKKNIEIVEDGENGPQSIIETSNK
                     LEGNGIVLTNDGYILTNKHVVQDKNAEYKVILQNQEFPVDKIRYDDGLDLAIIKIKVK
                     DYLTASKIKKIQDKNTIGQTVFALKKDPDIGETITKIGIINSLNQKFKMDNNNVYVGL
                     IKTSTAIEPGFSGGPLINLNGEIIGINTAIDNLEYGASYSLPINQEFINQTLSSIKQS
                     GQIIRPEIGIKYESNPVGIRIISIEEESNAQKAGLQINDIIVGINNVKVDYNNFLYEL
                     FTYNPEKQVILNIQRGSNKHEIQVSLGVKR"
BASE COUNT         1686 a          520 c          677 g         1083 t
ORIGIN      
        1 ttccatatcc attagaaaaa gaccttacac aatcttatat agattatgct atgtctgtaa
       61 ttatttctcg tgcattacca gatactagag atggttgtaa acccgtaatt agaagaatat
      121 tgtatggtat gtacgatatg aaaatgtttt acaatacaaa acacaaaaaa tcagctagaa
      181 tcgtatgaga tgtgatgtga aaataccatc cacacggaga tagttcgata tatgaagcca
      241 tggttcgtct tgctcaacct tggtctatga gatatccttt ggtagattga caaggtaact
      301 ttggtagtat ggacggagat ggggcagcag cgatgaggta tacagaagct agattgacaa
      361 aaatagctga agaaatgata aacgatatcg aacaagatac cgtagatcga agaaacaact
      421 ttgacggttc tttgcaagaa cccgttatgc tacccaccaa attcccaaac cacttatgta
      481 atgggacaat gggtattgct gtaggtatgg ctacaaactt agctccacac aacctaaacg
      541 aagtaatcga tgcttgtttg ctactgattc aaaaagaatg aaaagaagta gaggttccta
      601 tcgattttat agacaacaat gaaaatcttt cccaagatga atgagattta cagagcagtg
      661 acgaaaatat tacagaaaat acacaaaatg aagagcttcc aaatatcgat tccacaacga
      721 gtccagattc aacaaattca aatgggacta aaacaataaa atattctgta tctatagacg
      781 aaattatgga aattgtaaaa tgaccagatt ttcctacatg atgatatatt tttgatccaa
      841 atagaatcaa agaagtttat tcaaagggta gaggaggtat agtttgtaga ggcagacatg
      901 agatagaaga aataaaatca tgaaaaaata tagtaatcac agaaatacct tatatggtaa
      961 ataaatctac attagtatcc aaaatcggtg aattagttgt agataaaaaa attgattgaa
     1021 tagtagatat tagggatgaa tcaaataaaa ataaaattag agtcgtatta caacttagaa
     1081 aatgaataaa tccagatcaa attttgatcc aattatacaa agcaaccgat ttacaatcaa
     1141 attttaatgt aaacaatgtt tctttagttg acaatggact acaacctagg acactcaata
     1201 tcaaagagct tttgatggaa tttgttacat ttagaagaca agtagtttat cgtagatcaa
     1261 attatcaatt agataaagca agagataggt tacatatttt ggaatgacta aaaaaagcta
     1321 tcgatattat cgatgaagta atagaaacca tcaaaaagtc cgcaacaaaa caagaagcaa
     1381 aagaaaatct gatggcaaag tttgattttt cagaccaaca ggcagaatac attctactta
     1441 tgagattaca atctttagtt ggattggaaa tacaaaaaat atccgacgaa attgatgaaa
     1501 aaattaaact aattgaatat ttagaatcaa tcataaacaa ttcagaaaag ttggatgaag
     1561 tagtggtaga ggagctaaat tacataaaag aaaaatatgg agatgagaga aaaacagaag
     1621 tttccaacga tcttggcgtt tatagtcttt gaagcagtct aaaagcatac agagatgctg
     1681 cggataaaat aaaagaagat gtaattttgc gaataggaaa cgattatcat gtcagagttt
     1741 tgtatcaatc cagaattctt gctattccag aggaaacatt agagctgatc tatactcaca
     1801 atcaagataa tctaatagtg atcacagatt tgggggaact agtagtagaa agactaaaag
     1861 atttatgaca atttactatg gtaaaaaatg ctttggacct caaagagcaa tatggcctaa
     1921 aatgaaacat aatttttgcc aaaaccttgc attatcacta cgaacaccta ttatttctta
     1981 caaaagataa taatattaaa aaaataaaca aagatttaat cttaaaattc aaaaagtttc
     2041 caacaatcgt gatgaaattg gaagcaaaag aaaaaatcgc agcagttttg gcagtttctg
     2101 atggagacaa tgtagctata gtgacagaac aatgacgagg attgctgttc ccagcctcag
     2161 atatcagagc aatgtgaaaa actgctggat gagtcaaagc cattgattta caagaatgag
     2221 atagagtatc aaacatgttt ttacataaag aacaaccatt tttattagta cataacaaaa
     2281 aagaagctaa attactaagt ttagaagatt tgagagtacg aaaaagggct agaaaatgag
     2341 atgtttgggc tactggggat tgtaaattgg agtgaggtat tagtatagag gaatgagcga
     2401 ttagaatcag atttacggat tgaagcctca aaacattaca ttcaaatgat gtaaaattgg
     2461 atatgccaga tactcctcta aaagagattg taaaaaaaga tatagaattg atatacagac
     2521 cttgggaaga aaaagaagaa aatttcaaat gaaaagaaga aagaaaagca agagaaaaag
     2581 cagaaagaga agcagaagaa aaaagaaatg ccttattttc agatggagat attgagtagt
     2641 tttggggaca atactatatt ttaactagtt ttatactcat aagatataaa atgtgaaaaa
     2701 acaagatagg ttcaataatt ttaacaatgc taatgagtgt aataactttg ttggtattgc
     2761 tgttaatgat aaaaattagc aaactagaaa ctaaaatatc aattattacc tgagaagata
     2821 tcgtggatat cacccaacat caaaaagatt ttgatttcaa attattcgaa gaatcgatat
     2881 taaaaaattt atgagaacta gacaaaaata ttgtagcgat atatgccaaa aaaaacatag
     2941 aaatagtaga ggacggtgaa aattgacctc aaagcatcat agaaacaagc aataagctgg
     3001 aatgaaatgg tatagtatta accaatgatt gatatatttt gacaaataaa catgttgtcc
     3061 aagataaaaa cgctgagtat aaagtaatct tacaaaatca agaattccca gtggataaaa
     3121 tacgatacga tgatggattg gatttagcaa ttataaaaat caaagtaaag gattatctca
     3181 cagcatccaa aatcaaaaaa atacaagaca aaaacaccat ctgacaaaca gtttttgctt
     3241 taaaaaaaga tcctgatatc tgagaaacaa tcacaaaaat atgaattata aactcattga
     3301 atcaaaaatt taaaatggac aataacaatg tttatgtttg acttataaaa acaagcacag
     3361 ctatagaacc atgatttagt ggatgaccat taataaacct aaattgagaa ataataggga
     3421 taaatacagc aatagataat ttagaatatg gagcctctta ttccttgccc atcaatcaag
     3481 agtttataaa ccaaacttta tctagcatca aacaaagtgg acaaataata agaccagaaa
     3541 tcggtatcaa atatgaatca aatccagttg gaataagaat aatttcaata gaagaagagt
     3601 ccaacgctca aaaggcatga ttacaaataa atgatatcat tgtatgaata aataatgtaa
     3661 aggtagatta taataatttc ttgtatgaac tatttactta taatccagaa aaacaagtta
     3721 tattaaatat tcaaagatga agtaacaaac acgaaatcca agtaagctta tgagtaaaaa
     3781 gatagaaagt tacaagttat aaagttgaaa gtttataaag ttaaaagtta caaacttaac
     3841 acattttcca tagaaagtaa agtactaaat tcaaaagatg atggaattat ttgtttttta
     3901 tgctaaaaaa atgtatcatt gtattaacaa aatgatttat ttctaaaccc aaaacaaaca
     3961 tggtag
//
LOCUS       DAOSID010000069         3909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_20437_length_3909_cov_14.722107, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000069 DAOSID010000000
VERSION     DAOSID010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20437_length_3909_cov_14.722107"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PK674_03035"
     CDS             <1..270
                     /locus_tag="PK674_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="HU family DNA-binding protein"
                     /protein_id="HOG15541.1"
                     /translation="KTALIAQLAEELGVSKKLAAELVNTFIDTVIAGVKKEGEVRIQG
                     FGTFKKSHRKAREGVNPQNPSQKIKIPAMNVVTFKAGSDFKGAVK"
     gene            590..985
                     /locus_tag="PK674_03040"
     CDS             590..985
                     /locus_tag="PK674_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15542.1"
                     /translation="MNGYTFLAFVAGGLLGFFLFYFRYKYRDVVNELRGNLKSANKEL
                     EYLNHELDEFTHQNAILKEKVTELLDKNDELSEVVAELSKYYVHIKRASEKTSELTKF
                     LADPNPEIEEKMKNILKNSANSSNEKTFF"
     gene            1002..1640
                     /locus_tag="PK674_03045"
     CDS             1002..1640
                     /locus_tag="PK674_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG15543.1"
                     /translation="MAYNSKHVQILDPEKGYNLISDQYKQYHSHLDSFFNLEIFKFLP
                     REYENLDIIDLGAGDGRVFKYFENVSFRKYVACDIAEKLLRKHPGKGIKVQKIVCDLN
                     KKLPFDDEIFSIATSFFVLEHIENIEGLFGEVYRILKYGGKWIIGHFIQRREFEREHG
                     KGKDRMRFKIQQYHYRLEELKKIAEYNFFEFNYQEIVENGVLIGYLIVCKKK"
     gene            complement(1714..2136)
                     /locus_tag="PK674_03050"
     CDS             complement(1714..2136)
                     /locus_tag="PK674_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HOG15544.1"
                     /translation="MDYIWETSDVLLTGSISNELGLAALAGSVAVLGIFFVIFGLISI
                     FMIISMRKVLEKANLPGWGIFIPIYNIYLMFKLGGRPGGWTRWILFPPVLAILMIILN
                     FDIAKKFQKHPLFGVGLWFFPIVFFPILAFDKKSKWSK"
     gene            complement(2368..2949)
                     /locus_tag="PK674_03055"
     CDS             complement(2368..2949)
                     /locus_tag="PK674_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15545.1"
                     /translation="MEKSAIQERHDQLDQEILSFAKNENYALMKQKVDLQKKLSQILE
                     KGKQEEMVFDEKNLLDDQLYVKAKEVGDQKLLDKFHNRSLTRKEYVKACKDLNINLGI
                     DAYYSFLDKYDKIFDCNKYGLAVVQLDNKYGVINQESQEEIVKCSDCIYIGEFDEYGI
                     ASVTLRFFGLVQKRYINTQGEKFQKHPFTKKKG"
     gene            complement(2981..3769)
                     /locus_tag="PK674_03060"
     CDS             complement(2981..3769)
                     /locus_tag="PK674_03060"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="WG repeat-containing protein"
                     /protein_id="HOG15546.1"
                     /translation="MEKSTIQERHDQLDQEILSFAKNENYALMKQKVDLQKKLSQILE
                     KGKQEEMVFDEKNLLDDQLYVKAKEVGDQKLLDKFHNRSLTRKEYVKTCKDLDIDLGV
                     DYYYDSLVRFDKVGMFNEYGLADVKIGNKYGLINKEGEEITEIKYNMIFNFNKYGLTV
                     VQIGNKYGVINTEGKEICERMYDDIFDFDEYGLAKVKLGDNYGLINTEGKEIIKCDDC
                     TYIGEFDKCGLVEVEIDGERCYVNREGIIFRNFIDSFLYIYFDI"
BASE COUNT         1436 a          567 c          567 g         1339 t
ORIGIN      
        1 aaaactgctc ttatcgcaca attagctgaa gaattaggag tttctaaaaa acttgcagct
       61 gaactagtta acacttttat tgatacagtt attgctggtg ttaaaaaaga aggtgaagtt
      121 agaatccaag gattcggaac tttcaaaaaa tctcacagaa aagctagaga aggagttaac
      181 ccacaaaatc catctcaaaa aatcaaaatc cctgctatga atgttgttac tttcaaagct
      241 ggatctgact tcaaaggagc tgttaaataa tagcgactta aaaagttttt atacaaagaa
      301 tctcctactc gttgggaggt tttttggaaa taaaaaaaat actttttttc tcataatacc
      361 ttttcatacc acccggcccc gtagccccgc aatactgcgg ggcggggcgg gaagagcatc
      421 ctcccgaacc ccgatctatc ggggccaaaa gggaggaatt tcaccctaaa actttacaaa
      481 ccacgcagta ctgcgtggca ggctttacaa actcaacaaa ctttcctgtt gattttggag
      541 aactaaaatg taatttatta ttgtatttat ttatcaaaaa ataacgcaaa tgaattgata
      601 tacttttttg gcttttgtag cttgatgatt actgggattt tttttgtttt attttagata
      661 caaatataga gatgtggtga atgagttaag atgaaatcta aaatcagcaa ataaagaact
      721 agaatattta aatcacgagt tagatgaatt tacacatcaa aatgcgatac taaaagaaaa
      781 agttacagaa ttattggaca aaaatgatga attatctgag gttgttgctg aattgagtaa
      841 atattatgtt catatcaaga gagctagcga aaaaactagc gaacttacaa aatttctagc
      901 agatccaaat ccagaaatag aagaaaaaat gaaaaatatt ttgaaaaact ctgcaaattc
      961 aagtaacgaa aaaacattct tttaatagga ttttgataaa tatggcatat aattccaaac
     1021 atgtacaaat attggatcca gagaaatgat ataatttgat ttcagatcaa tacaaacaat
     1081 atcattcaca tctggattct ttttttaatt tagaaatatt caagtttttg cccagagaat
     1141 atgaaaatct ggatataata gatttggggg catgagatgg tagagttttc aaatattttg
     1201 aaaatgtgag ttttagaaaa tatgtagctt gtgatattgc tgaaaaactt ttgagaaaac
     1261 atccagggaa atgaattaag gtacaaaaaa tagtatgtga tttaaacaag aaactaccat
     1321 ttgatgatga gatttttagt attgcaacaa gtttttttgt actggaacat atagaaaata
     1381 tagaatgatt atttggagaa gtgtatagga ttttaaaata ttgatgaaaa tggataattg
     1441 gtcattttat acaaagaaga gaatttgaac gagaacattg aaagtgaaaa gatcgtatga
     1501 ggtttaaaat acagcaatat cactatagat tagaagaatt gaaaaaaata gctgaatata
     1561 atttttttga atttaattat caagaaattg tggaaaattg agttttgata tgatatttaa
     1621 tcgtatgcaa aaagaaatag aaaaaacata acatgtttta aagaaaaaat gaaattacgc
     1681 gaaataaaaa gagaaacatt aaatttagaa cgattattta ctccatttag attttttgtc
     1741 aaaagccaat attgggaaaa agactattgg gaaaaaccaa agtcatactc caaataatgg
     1801 gtgcttttga aactttttgg cgatatcaaa atttaaaatt atcatcaata ttgctaaaac
     1861 tggtggaaac aatatccatc gtgtccatca gccaggtctt catcataatt taaacattag
     1921 ataaatgttg taaattggta taaaaatacc ccatcctggc aaattagctt tttctaaaac
     1981 ctttcgcatt gaaataatca taaaaataga aatcaatccg aaaataacaa aaaatattcc
     2041 gagtacagca actgatcatg cgagggcggc caagcctagt tcgttggata tagaaccagt
     2101 aagtagtaca tcagaagttt cccaaatgta atccatgatt gttgaaaaat gaaataaaaa
     2161 ttacacttaa tgtatttagt atataataaa aagatggaaa tttacaagtg gaaaaaatat
     2221 aattttgatt ataagcaaaa tatgtgactt actagtacta acaaggggat ttttgtgttg
     2281 gtggataatt atagtgatat ggaaacaaga aaacaaaaaa gacggaagcg attaacttct
     2341 gtctttaggg gattatttat ggatttgtta tcattttttc tttgtgaatg gatgcttttg
     2401 aaacttttct cattgggtat tgatatatcg tttttgaact aaaccaaaga aacgcaaagt
     2461 tactgatgca ataccatatt catcaaattc tcaaatataa atacaatcac tacatttgac
     2521 aatctcttct tgggattctt gattgattac tcaatattta ttgtcaagtt gtactacagc
     2581 aagaccatat ttattacaat caaaaatttt gtcatattta tctaaaaaac tgtaataagc
     2641 atcaattcat aagttaatat taagatcttt acaagctttt acatattctt ttcttgttaa
     2701 gctacgattg tgaaatttgt ccaaaagttt ttgatctcct acttcttttg cttttacata
     2761 aagttggtca tccaaaagat ttttttcatc aaaaaccatt tcttcttgct ttcctttttc
     2821 aaggatttga gagagttttt tttgtaaatc aactttctgt ttcatcagag catagttttc
     2881 gttttttgca aaactaagaa tctcttgatc aagttggtca tgtctctctt gtatcgcact
     2941 tttttccatt gttgtaatga taaaatttaa agttattaat ttagatatca aaatatatat
     3001 ataaaaagga gtcaataaag ttcctaaata ttattcattc tctgttgaca tagcatcttt
     3061 ccccatctat ttctacttca acaagaccac atttatcaaa ctctcaaatg taagtacaat
     3121 catcacattt gatgatctct tttcactctg tattgatcaa tccataatta tcaccaagct
     3181 tcactttagc aagaccatac tcatcaaaat caaagatatc atcgtacatt ctttcacaaa
     3241 tctcttttca ttctgtattg attactcaat atttattccc aatttgtact acagtaagac
     3301 catatttgtt aaaattaaaa atcatattat actttatttc agtaatttct tctcattcct
     3361 tgttgattaa tccatattta tttccaattt ttacatcagc gagaccatat tcattaaaca
     3421 taccaacctt atcaaatcta actaaagaat cgtaatagta atcaactcat agatcaatat
     3481 ctaaatcctt acaagttttt acatattcct ttcttgttaa gctacgattg tgaaatttgt
     3541 ccaaaagttt ttgatctcct acttcttttg cttttacata aagttggtca tccaaaagat
     3601 ttttttcatc aaaaaccatt tcttcttgct ttcctttttc aaggatttga gagagttttt
     3661 tttgtaaatc aactttctgt ttcatcagag catagttttc gttttttgca aaactaagaa
     3721 tctcttgatc aagttggtca tgtctctctt gtatcgtact cttttccatg cttgtaagtt
     3781 gtaataagta aaagttaaaa aatctaaact gcaattataa taacaattta caacataaag
     3841 tcaagcgtat tttatttttt attaattata attcaaaaag caccactaat agataagata
     3901 ataaatggg
//
LOCUS       DAOSID010000070         3806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_21121_length_3806_cov_13.126100, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000070 DAOSID010000000
VERSION     DAOSID010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21121_length_3806_cov_13.126100"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..1845
                     /locus_tag="PK674_03065"
     CDS             304..1845
                     /locus_tag="PK674_03065"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HOG15547.1"
                     /translation="MKQVIITKSLFKEFCENPKLARWHKNDYVIYNKINQELYGAMNG
                     ALMGQEVENMVKKLRKTKDISVVAGYNPKGNFHKEYSDATQKTLSFDPKIVYQAGFVW
                     DNLFIKCDFLKLNEHGKYDLIEVKAKNSIHKSSKNQSLLDDLITDCSFQKHVLQKTLG
                     DKFSGNVWIYHLNKEYLRQGDIAPNEIIAKENVTEELFTNDWVEKQIDLINNSICLSK
                     QDFEQKFPYQGENPLIYFGKSPKNGTIWNIPYFKTAKKKLLSLYKIGKTNIMDLGKNE
                     LKLISSKDELENSFQKYITMFQKGVQFDKEKISSTLNNLKFPLFFYDYETIVWPIPIL
                     QGTHPRQQVVVQYSIHKMDLNGKISHHEGIISPFSQTNKNVIDKFVNDIGNPKGTFVV
                     RNKGFENSRNQEIGEMYPEYKKFFEQINSNTFDLMDVFRNLDYFDPAFDGSCSIKKVL
                     PVLSDISYDDMTVSNGSEASDYLYQLIQNKLPIQVSTQDLLDYCKQDTRAMVEIYNFL
                     KRKVS"
     gene            complement(1951..3012)
                     /gene="mnmA"
                     /locus_tag="PK674_03070"
     CDS             complement(1951..3012)
                     /gene="mnmA"
                     /locus_tag="PK674_03070"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOG15548.1"
                     /translation="MKKILIGLSGGVDSAIATYLLKKQGYHITAGFMKNYIDEENPNC
                     STYQDAKEAIKVAEFLGIEIISFDLRQEYEEKIIKYIYDGYQKGITPNPDILCNNLIK
                     FDVFLEKALKLGFDGIATGHYARIVPILDNGGMGYKLLRGVDYNKDQSYFLSGLNQYQ
                     LSKTLFPIGDMTKNEVRELAKKIGLPNFDRPDSQGLCFVGNVPMREFLKKRLAVKKGD
                     IVLLDGTKVGEHDGAYFFTIGQSRGLNINKKAYVVKTDVEKNLVYVSYKKLEKELISK
                     EIFISDRHWIRKKYNFPLECTTKIRYRQETQKATIFEINEKEKKLKVIYKNDQWGIAP
                     GQTLTAYIDDECVGSGLIV"
     gene            3238..>3806
                     /locus_tag="PK674_03075"
     CDS             3238..>3806
                     /locus_tag="PK674_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15549.1"
                     /translation="MNEVYHLFKHIKSAKNHFQKHGKKYLFGTLGTFAIVKMVLLFMG
                     FFSTLDLGKSFAQTPVVLSKDNIVSMGCATDNISCNLSNKWITNIENDAFVNHIQLQT
                     LDLSFNKLNSLPENIVQLINLQDNGGLNINFNHIYYEGLSSGLLSFIDQKSSAGWRST
                     QTIVRGCTDPTAYNYDPDATNNDWTCKYPP"
BASE COUNT         1386 a          554 c          495 g         1371 t
ORIGIN      
        1 gttagtatca tactaacgtt tttcttttaa atattttttt tcccggacac tactgaaatt
       61 acagtaaaaa acgaaaattg tgacaaaaaa aattgtgatt ttaaaaaagg attttgttgg
      121 cacaaaaata tacaaaatct agtcctaatt ttgttaaaca gtataatctt acaaattatt
      181 acccaaagga gcatttttta aatttacaaa aaaagtattt caattctttt tgaattatgt
      241 acacataata atgttgtttt tttttggcct attttttaat taatttacaa ttaaatagta
      301 ttcatgaaac aagttattat aacgaaatca cttttcaaag agttttgcga aaatccaaaa
      361 cttgcacgat ggcacaaaaa tgattatgtt atttataata aaattaatca agaattatat
      421 ggtgccatga atggagcttt gatgggtcaa gaagtagaaa atatggtaaa aaaacttcga
      481 aagacaaagg acattagcgt ggttgccggt tataatccaa aatgaaattt tcacaaagaa
      541 tatagtgacg ccacacaaaa gacactttct tttgatccca aaattgttta ccaagctggt
      601 tttgtctggg ataatctttt tatcaaatgc gattttctaa aattaaatga gcactgaaaa
      661 tacgatttga tagaagtaaa agcaaaaaat agtattcata aatcaagcaa aaaccaatca
      721 ttattagatg atttaattac agattgtagt tttcaaaaac atgttcttca aaaaacactt
      781 tgagacaaat tttctggtaa tgtttggata tatcatttaa ataaggaata tcttaggcaa
      841 tgagatatcg cccccaatga aattatagca aaggaaaatg tgacagagga attgttcaca
      901 aatgattggg ttgagaaaca gatagatttg ataaacaaca gtatttgctt gtccaagcag
      961 gattttgaac aaaaattccc atatcaatga gaaaatcctt tgatatattt ttgaaaatct
     1021 cccaaaaatt gaacaatttg gaatatccca tattttaaaa cagcaaagaa aaaattatta
     1081 tcattatata agatatgaaa aacaaatatt atggatttag gaaaaaatga gttgaaacta
     1141 atttcttcaa aagatgaatt agaaaacagt tttcaaaagt atattacaat gtttcagaag
     1201 tgagtccaat ttgataaaga aaaaatttca tccactttaa ataaccttaa attccctttg
     1261 tttttttatg attatgaaac tattgtgtgg ccaattccaa tacttcaatg aactcatcca
     1321 cgacaacaag ttgttgtaca atattctata cacaaaatgg atttgaatgg gaaaatttca
     1381 catcatgaat gaattatttc tcctttctct cagacaaata aaaatgtgat agataaattt
     1441 gttaacgata tttgaaaccc caaagggaca tttgttgttc gaaataagtg atttgaaaat
     1501 tctagaaatc aagaaatatg agagatgtat ccagaatata aaaagttttt tgaacaaata
     1561 aactcgaata catttgattt gatggatgta tttaggaatt tagattattt tgatccagca
     1621 tttgatggtt cttgtagtat aaaaaaagtc ctgccagtct tgtcggatat tagctatgat
     1681 gacatgacag tttcaaatgg aagtgaggct tcagactatt tgtatcagct tatccaaaac
     1741 aaactcccca tccaagtttc tacacaagac ctactggatt actgtaaaca agatacacga
     1801 gctatggtag aaatttataa ttttttgaaa aggaaagtat cctaagtaaa aaagctcata
     1861 aagtttataa attatttata acttcataac ttgcaacttg taattttata acttgcaact
     1921 tgataaactt ttagctttat gaacttttta ctatactata agcccactgc caacacactc
     1981 atcatctata tatgcagtta aagtttgtca gggagcgatt caccattgat catttttata
     2041 tatcactttt agtttttttt ctttctcatt tatttcaaaa attgtagctt tttgagtttc
     2101 ttgtctatat ctaatttttg tcgtgcattc cagaggaaaa ttatactttt ttcttatcca
     2161 atgccggtca cttataaaaa tttctttact aattaattct ttttcaagct ttttataact
     2221 cacataaact aaatttttct ctacatctgt ttttactaca taagcttttt tgtttatatt
     2281 caaacctcta ctttgtcaaa tagtaaaaaa atatgctcaa tcatgttctc agaccttagt
     2341 tccatctaac aaaactatat cacctttttt tactgctaat ctttttttca aaaattctct
     2401 cattggcaca tttcaaacaa aacataatca ttgactgtct ggacgatcaa aatttggtaa
     2461 tcaaattttt ttagctaatt ctctaacttc gttttttgtc atatcaccga taggaaacaa
     2521 agttttactc agttgatatt ggtttaatca tgataaaaaa taactttgat ccttattata
     2581 atccactccg cggagaagtt tataacccat tcctccatta tctaatattg gaactattct
     2641 agcataatgt cctgtagcaa ttcaatcaaa tccgagtttc aatgcctttt ctaaaaaaac
     2701 atcaaactta attaaattat tacataaaat gtctggattt ggtgtaattc atttttgata
     2761 tcaatcataa atatatttta ttattttttc ttcatattct tgtctgagat caaaactaat
     2821 tatttctatt cataaaaatt ctgctacttt gattgcctcc ttagcatctt ggtaagtaga
     2881 acaattggga ttttcttcat ctatatagtt tttcataaat cccgccgtga tgtgatatca
     2941 ttgttttttc agtaaataag tagctatagc actatctact caaccactta atcatatcag
     3001 tatttttttc atgtaataac tcttttaaat aaagcataat tatgcactaa atatatatta
     3061 tttttaataa tttttgcaat tacattttag ttattcgttg tttatgttga tatgttttat
     3121 cagcattaca caagataact taatttctat aaaaaagtcc ctcaaaattt gacaggattt
     3181 tttttttgaa tataatcgac tctagaatct tttttattca tatttgtttg acaaaaaatg
     3241 aacgaagtat accacttatt taagcacata aaaagtgcta aaaatcattt ccaaaaacat
     3301 ggaaagaagt atttatttgg gacactatga acattcgcta tagtcaaaat ggtgttgttg
     3361 tttatgtgat ttttcagtac actagatttg ggtaaaagtt ttgcacaaac accagtagta
     3421 ctaagtaaag ataatattgt atcaatgggc tgtgctactg acaatatttc ttgtaattta
     3481 tctaataaat ggattaccaa tatagaaaac gatgcttttg taaatcatat acaattacaa
     3541 actttagatc tgagtttcaa taaattaaat tctctaccag aaaatattgt tcagttaata
     3601 aatttacaag acaattgatg attaaatatt aattttaatc atatatatta cgaatgattg
     3661 tcatcaggac ttctaagttt tatagatcaa aagtcaagtg caggatggag gagtacacaa
     3721 actatcgtac gatgatgtac agatccaaca gcatacaatt acgacccaga tgcaacaaat
     3781 aatgattgga cttgtaaata tcctcc
//
LOCUS       DAOSID010000071         3737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_21629_length_3737_cov_14.389191, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000071 DAOSID010000000
VERSION     DAOSID010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21629_length_3737_cov_14.389191"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="PK674_03080"
     CDS             <1..1330
                     /locus_tag="PK674_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15550.1"
                     /translation="AESGYLTRKLCDASQEVVIRSEDCKTDKKILITKEESELKGEDF
                     FENIYGRVLAQDITDLKGTIILKKGDLLDKENISLLKKLEIAEIYYKSPLICMEPSGV
                     CQKCYGMDLATRKLVDIGVPVGIIAAQSIGEPATQLTMKTFHHGGVAGGADMSQGIDR
                     VTQLFEVRPPKKSAVVAPFDGIVSFSEKGADNYINIASDYQKKSYILKDNYKFTVKKG
                     DIIKKGGEYASKGSSKLKVQEEGTVLEIKKDSMSFGIKESFKKSYKGLVLLCKEGDYV
                     YKGQLLTNGPLDVNEYKDIVGDLEAQKYIIKEVQKVYADQGQGLNDKHTEVVVKQLFS
                     KVFIERPGDSGFIPGTHVKYEDFVKMNNELESQGKLPAEGRRLALGLTSIAKGTDSWL
                     SAASFQETIRVMVGASLRGAIDNLSDLKSNVIIGRLLPVGEIYKKEHGYE"
     gene            1446..1658
                     /locus_tag="PK674_03085"
                     /pseudo
     CDS             1446..1658
                     /locus_tag="PK674_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002778821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=25
                     /product="50S ribosomal protein L27"
     gene            2350..2892
                     /locus_tag="PK674_03090"
     CDS             2350..2892
                     /locus_tag="PK674_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOG15551.1"
                     /translation="MKVGNRINARKIVLSYFYQRCFFINLEKQINLISSDILFVENIF
                     MSQGELFDKAKKELEQKIRSKMQIDEDEDLEYFINIFFNKRDVENIDMDYVFKVGSVF
                     EKYYQKVVSQVNSHTQSFSFPEMNTMNQALFVLGYIERKVLETPKEILLNELIELSKR
                     YDDGGSPKLINGIMHNIVNG"
     gene            2993..3658
                     /locus_tag="PK674_03095"
     CDS             2993..3658
                     /locus_tag="PK674_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15552.1"
                     /translation="MKNFLILLLLLVVAGFVFLFLEPEHDLSQKVFGYLNITVSKNVE
                     VEVDLTDCISYFDGCNTCMVEDGEIKGCTRMFCDTTEEPRCLEYKWTGMDLTNCLSYF
                     DGCNTCEVKNGEISLCTEISCEEYQEAKCTQYVSDVISSEDEMDLTNCLSYFDGCNTC
                     EVKNGEISLCTEISCEEYQEAKCTQYVSDVISSEDEIDTSSVEAEIDLPEDVDPLESE
                     TVE"
BASE COUNT         1525 a          431 c          637 g         1144 t
ORIGIN      
        1 agcagaatct tgatatctta ctagaaaact ttgtgatgct tctcaagaag ttgttataag
       61 atcagaagac tgtaaaacag acaaaaaaat tcttattaca aaagaagaat cggaactaaa
      121 gtgagaagat ttttttgaaa atatatattg aagagtttta gcacaagata ttacagacct
      181 aaaatgaacg ataatattaa aaaaatgaga tttattggat aaagagaata tatctttact
      241 aaaaaagtta gaaattgctg aaatatatta caaatctcct ctgatttgta tggagccaag
      301 tggtgtctgt caaaaatgtt atggtatgga tcttgctact agaaagttgg ttgatatatg
      361 agtcccagtt tgaataattg cagctcagtc tatatgagaa cctgctactc aattaactat
      421 gaaaacattt caccatggtt gagtagcatg aggagcagat atgtcacagg gtattgatag
      481 agttacacag ctttttgagg taagaccacc aaaaaaatcg gcagtagttg ctccatttga
      541 ttgaattgta tctttttctg aaaaaggagc ggacaactat attaatatag catctgatta
      601 tcaaaaaaaa tcttacatat taaaggacaa ttataaattc actgttaaaa aatgagatat
      661 tataaaaaaa tgatgagaat atgctagcaa gggatccagt aagttaaagg ttcaagaaga
      721 gtgaacagta ttagaaatca aaaaagattc aatgagtttt ggtatcaaag agtcatttaa
      781 gaagtcctat aaatgattgg tccttctttg taaagaatga gattatgttt ataagtgaca
      841 actattaaca aattgaccac tagatgttaa cgaatacaaa gatatcgtat gagatttgga
      901 agctcaaaaa tacataataa aagaagtaca gaaagtttat gctgatcaat gacaatgact
      961 taacgataag catacagaag tagtcgtgaa acaattgttt tcaaaagtgt ttatagaaag
     1021 accaggtgat tctggattta taccgtgaac tcatgtaaaa tatgaggatt ttgttaaaat
     1081 gaataatgaa ttggaatctc aatgaaaatt accagctgaa tgaaggagat tagccttggg
     1141 tctcacaagc atcgcaaaat gaacagattc ttggttatct gctgcatctt tccaagaaac
     1201 aattagggta atggtcggtg catctttgag atgagcaata gataacttat cagatctaaa
     1261 gtctaatgtg ataatttgaa ggctattgcc tgtaggtgaa atatataaaa aagaacattg
     1321 atatgaataa atgggaattt tagtttctat ttaaatatta taagctcaag tatttaatct
     1381 tgacaacaaa acttgaatta gtataagata actcattgtt ttgatttata aattaatgaa
     1441 taattatggc tcataaaaaa gcagcgtgat ccgcaaaaaa ccttagagac tcaaaaccaa
     1501 agtatagatg agtaaaggta ttttgatgac aaaatgttct tgcatgaaac attattttaa
     1561 gacaaaaatg aactaaatat caagttggta aaaatacata tatgtgaaaa gatttttcta
     1621 ttcatgcagc tattgatgga gtggttgagt tttcaaaagc taatatttct aaatttgatg
     1681 gaagaaaata tctcaaaaca tttgtaaatg ttgtttcatc aagttttatt gaaaaaaata
     1741 aatgattaga aaaaagagaa atcaaaaaag atatcaaaaa agaaactgaa aaagagacaa
     1801 aggatcaagc tgttatcaaa aaagaaacca caaagaggcc aactattaca aaaaaaccat
     1861 cgatcaccac tccaaaagta gtcaaaaaaa cagatgttgc taaaaagaca gttagtacca
     1921 aaaaagtaac taccaccaaa aaagcagata ctaaaaaagt agctagtacc caaaaaacaa
     1981 atactgctaa aaaaacaact agtaccaaga aagcaactac cacaaaaaaa ccaactacca
     2041 aaaagctaag caaatagata tatttatttt tttagttaaa tttaaatggc aggaattagt
     2101 cctaaaaaaa agattacaaa gagcgttaga aatacaagac attcaacacg ggaaagattg
     2161 cagttaaaaa agttatcaaa caaatattct gtgtcaaaat gtaataattg tggtaaaccc
     2221 aaactatcac atagagtgtg tcctcattgt tgatactaca aaggtaaaca agttattaca
     2281 atcaaaacca aaagtaaaga tacagtagtt gatgcttaaa agtttaacaa ttaaagcaaa
     2341 ctttaaaaca tgaaggtttg aaatagaata aatgcaagaa aaatagtgtt gagttatttt
     2401 tatcaacgct gtttttttat taatttagaa aaacaaataa atttaatatc tagtgatatt
     2461 ttatttgtgg agaatatttt tatgtctcaa tgagaactat ttgataaagc aaaaaaagaa
     2521 ttagagcaaa agattagatc aaaaatgcag atagatgagg acgaggactt agaatatttt
     2581 ataaatatat tttttaataa acgagatgtg gaaaatatag atatggatta tgtttttaag
     2641 gtttgatcag tatttgaaaa gtattatcaa aaagtagtat ctcaggttaa ttcccacaca
     2701 caaagttttt cttttccgga aatgaataca atgaatcaag ctctctttgt cctttgatat
     2761 atagagcgaa aagttttgga aactcccaaa gaaatattat tgaacgagtt aatagaattg
     2821 tccaaaagat atgatgatgg atgatccccg aagttaataa attgaattat gcataatata
     2881 gttaatggtt agtttataaa caacaatcct accacctgga tgtaaatagt tagttacaaa
     2941 taacactcga gtggtaggat aaactgtttt ttatcctttt aaaatataaa aaatgaaaaa
     3001 tttccttatt ttattattgc tattagttgt agcctgattt gtgtttttat ttttggagcc
     3061 agaacacgat ttatctcaaa aagttttcgg gtacttgaat attacagtaa gtaaaaatgt
     3121 tgaagtagaa gttgatttaa cagactgtat aagttacttt gattgatgta atacttgcat
     3181 ggtagaggac tgagaaatta agtgatgtac aagaatgttt tgtgatacta cagaagaacc
     3241 aagatgcttg gaatacaaat ggacatgaat ggatttaaca aattgtttat cttattttga
     3301 tggctgtaat acttgtgaag taaaaaatgg agaaatatca ttatgtacgg agatttcttg
     3361 tgaagaatat caagaagcaa aatgtacaca atatgtttca gatgttatta gttcagaaga
     3421 tgaaatggat ttaacaaatt gtttatctta ttttgatggc tgtaatactt gtgaagtaaa
     3481 aaatggagaa atatcattat gtacggagat ttcttgtgaa gaatatcaag aagcaaaatg
     3541 tacacaatat gtttcagatg ttattagttc agaagatgaa atagatacta gttctgtaga
     3601 agcagaaata gacttaccag aagatgtaga tccattagag tctgaaactg tggaatagtt
     3661 gattgaataa taggaatata gctaaactta taatttttat acaaaatatt ttatataagt
     3721 ttttagataa aataatt
//
LOCUS       DAOSID010000072         3667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_22175_length_3667_cov_10.922757, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000072 DAOSID010000000
VERSION     DAOSID010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22175_length_3667_cov_10.922757"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..231)
                     /locus_tag="PK674_03100"
     tRNA            complement(142..231)
                     /locus_tag="PK674_03100"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(195..197),aa:Ser,seq:tga)
     gene            394..879
                     /gene="def"
                     /locus_tag="PK674_03105"
     CDS             394..879
                     /gene="def"
                     /locus_tag="PK674_03105"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="peptide deformylase"
                     /protein_id="HOG15553.1"
                     /translation="MINLKKFYPIQTGLDNKILRKKSKTIDSIDQDILEFAEILMELM
                     YEYDGVGLAAPQIGRNVRMIAITYWKETKKGHERIDEDIMINPEIIEKSNEMLVSEEA
                     CLSVPDVIGKVKRHQNIVVEYLDIFGRKKKKKLRNYNACIVQHEIDHLDAILFVDKLI
                     K"
     gene            complement(980..1056)
                     /locus_tag="PK674_03110"
     tRNA            complement(980..1056)
                     /locus_tag="PK674_03110"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1020..1022),aa:Cys,seq:gca)
     gene            complement(1208..2263)
                     /locus_tag="PK674_03115"
     CDS             complement(1208..2263)
                     /locus_tag="PK674_03115"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOG15554.1"
                     /translation="MGNIKQANIETYEYAKKLLFASEVVVFPTETVYGLGANALDTKA
                     VEKIFILKNRPSNNPLIVHVGEKSEIQKYAIISNSIEEKIIEKLMPGSITVLLKKKEN
                     IPSVISQNPYVGIRIPSNRVALEFLRHVNLPIAAPSANISTKPSPTSAQMVKEYFDEK
                     VQLIIDGGDCSVGIESTVVKVDELFVHPITGDSNGEVFYKIVIHRPGFVTKEDFETLF
                     SDNQNVFVEYSSDISNFSPGNMYKHYSPNASIKIIENLEEIGNISNNIENKKIALILT
                     KETVEKYQNIISGLSKNIKVLFRGSQKNLIVCAQNLFKLYHQCDQEGIDLVFIEGLKE
                     EGLGFSIMNRVKKSAGS"
     gene            complement(2312..>3667)
                     /gene="der"
                     /locus_tag="PK674_03120"
     CDS             complement(2312..>3667)
                     /gene="der"
                     /locus_tag="PK674_03120"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOG15555.1"
                     /translation="LYKLTTSTMFQIGLVGATNVGKSTIFNRLIGQFRAIVTDIPGTT
                     TDIIFHDIHVDSIGDVRFADSPGLIEFEEERSYIKKIIDESDLILFVIDDSVGVTAKE
                     QKIYQYILDSNRRKQTVLLINKLDINYKPNELSLGIADYYDLGVEKIIGISAKNNLNI
                     TEIEDFIIDYFKDKEGRTDNVKIDNPIGIAIIGKPNVGKSTLLNTLVGTELAKVEDYL
                     GTTRDYITGEFTMGGKKYKIYDTAGIRRKGSIHGIEKIAYDKIKGMLKYVRPAVIFML
                     DAEQGLTHRDMTLLSEINDLALPVIFAINKMDLLDDVQQKMIKKQVQATLDFAKYIPI
                     APIISTDGTGVREMISMVSKIKKEAEKRIDTNELNRAINAELLQRPPRFPKNKICKIY
                     YITQVDINAPTFVVFVNKKERANFALKKWIENSIRKNFGFVGTPIVIRFKESGDDRDK
                     K"
BASE COUNT         1285 a          607 c          490 g         1285 t
ORIGIN      
        1 tacaaagttt ctggtcattt ctggaactta tctgcatata ctataactag tacaaatgca
       61 aacaataaaa ataaagtgat tgaaaatttt aaataattta atcctttgtt tctaaatgtc
      121 atagaataag ttgatagtaa atggcggaga gggtgggatt tgaacccacg gtccgcgtga
      181 acggacaaca gttttcaaga ctgccgcatt caaccactct gccacctctc cacaaatttg
      241 tgtattacac agagtatcta tatgttttgt cgaacacggt tgctaataat aatacatatt
      301 ttgaattgat tttcaagtat taaaaaataa aatgcatttt agtgctttaa aagtaattat
      361 ttttaatatg attagtttat ttttttcaaa aaaatgatta atttaaaaaa attctaccca
      421 atacagacat gattggacaa taaaatccta agaaaaaaga gtaaaacaat agattctata
      481 gatcaagata ttttagagtt tgcagaaatt ttgatggaat tgatgtacga atacgattga
      541 gtatgactag cagccccaca aatatgaaga aatgttagaa tgatcgctat tacctattgg
      601 aaagaaacta aaaaaggaca cgaacgaata gatgaagata ttatgataaa cccagaaatc
      661 atagaaaaat ccaacgaaat gttggtttca gaagaggcct gcctatcagt ccctgatgta
      721 atttgaaaag tgaaaagaca tcaaaatata gtagtagaat atttggatat ttttggtaga
      781 aagaaaaaga aaaaactcag aaattacaat gcatgtatag ttcaacatga aattgaccac
      841 ttagatgcga tactatttgt ggataaacta attaaataag caaattatta caataatata
      901 acaaaaaatc tcctttctat aaggagtttt ttttgagaga cggaactgag ttttttctct
      961 tgattctcgc tagagtaaat ggaggcaccc accggattcg aaccggtgaa ccaaggtttt
     1021 gcagacccgt ctattagacc actctagcag ggtgccatac agtctaaata aaccaacaaa
     1081 gctattgtta aatattcata aatctttttc aagacaaagt ttagtatgaa gtaccacccg
     1141 ggtgtgaaaa ctgaccccgg gataactccc ggggtcaaaa acagcaccca ggtggtaggt
     1201 agagtaacta gcttcctgca gactttttta ctctattcat aatagaaaat cccaatcact
     1261 cctcttttaa tcattctata aatactaaat caattcattc ttgatcacat tgatgataaa
     1321 gtttgaataa attttgcgca caaacaatta aatttttttg cgatcatcga aataaaactt
     1381 taatattttt ggacaatcag gaaataatat tttgatattt ttcaacagtt tctttggtca
     1441 gaattaatgc aattttttta ttttctatat tatttgaaat gtttcatatt tcttccaaat
     1501 tttcaataat tttgatactg gcattgggag aataatgctt atacatattt catggagaaa
     1561 agttggagat atcagagcta tattctacaa aaacattttg attatctgaa aacaaagttt
     1621 caaaatcctc ttttgtcaca aaacctggac gatgtattac aatcttataa aaaacttctc
     1681 aattactatc tcaagtgata ggatgaacaa aaagttcatc aacttttacc acagttgatt
     1741 ctataccaac actacaatct catcaatcaa taattaattg aactttctca tcaaaatatt
     1801 ctttaaccat ttgtgcagaa gttggactag gttttgtcga aatattagca cttggagctg
     1861 ctattggtaa attaacatgc ctcaaaaatt ctaaagcaac tcgatttgat ggaattctaa
     1921 ttcagacata aggattttga gaaataacac tcggaatatt ttcttttttt ttcaaaagaa
     1981 ctgtaattga accaggcata agtttttcta tgattttttc ttctatagaa ttggaaatta
     2041 tagcatattt ttgaatttca cttttttctc aaacatggac aatcaaagga ttatttgaag
     2101 gtctattttt aagtataaaa attttttcta cagcttttgt atccaaagca tttgcaccaa
     2161 gtccatagac agtttctgta ggaaaaacta caacttctga tgcaaacaaa agttttttag
     2221 catattcata tgtttctata tttgcttgtt taatatttcc cattaaaaaa acatttatct
     2281 actaaatatt tcacaaataa aatatcaaaa attatttttt atctctatcg tcaccactct
     2341 ctttgaaacg aataactatc ggtgttcata caaaaccaaa attttttcta attgagtttt
     2401 ctatccattt tttcaaagca aaattagctc tttccttttt attcacaaaa acaacaaatg
     2461 ttggagcatt aatatctacc tgcgtaatat agtaaatttt acaaatttta ttttttggaa
     2521 accttggtgg tctttgcaac aattctgcgt ttatagccct atttaattca tttgtatcaa
     2581 ttcttttttc agcctctttt ttaatttttg agaccatact tatcatttct ctaactcctg
     2641 taccatcagt agaaataata ggagctatcg gtatatattt agcaaaatcc aatgtcgctt
     2701 gaacctgctt tttaatcatt ttttgttgga catcatccaa aagatccatc ttattgatcg
     2761 caaatataac cggtaaagct aaatcattga tctcagaaag caatgtcata tccctatgag
     2821 taagtcattg ttcggcatcc agcataaaaa tcacagcagg tctaacatat ttcaacattc
     2881 atttgatttt atcgtatgca attttttcta taccatgtat acttcatttt cttctaattc
     2941 cagcagtatc ataaatttta tatttttttc ctcacatagt aaattctcca gtgatatagt
     3001 cacgagtagt ccccaaataa tcctccactt tggcaagctc agttcaaact aaagtattca
     3061 aaagtgtaga tttcccaaca ttaggctttc aaataatagc tattcatata ggattgtcta
     3121 ttttgacatt gtccgtcctt cattctttat ctttgaaata atctattata aagtcctcta
     3181 tctcagttat attcaaatta ttttttgcac taattcatat aattttttct actcacaaat
     3241 cataataatc cgcgataccc aaacttaatt catttggttt gtaattgata tctagcttgt
     3301 ttataagcaa tactgtctgc tttctcctat tggaatcaag aatatattga tatatttttt
     3361 gctcctttgc cgtaacacca actgaatcat ctatcacaaa aaggatcaaa tctgattcat
     3421 ctataatttt tttgatataa gatcgctcct cctcaaattc aattaatcaa ggactatctg
     3481 caaacctaac atctcaaata ctatctacat gaatatcatg aaaaataata tctgtagtag
     3541 taccagggat atcagttact atagctctaa attgtcctat caatctatta aaaattgtag
     3601 attttcaaac atttgtagct caaacaagtc atatttgaaa catagtggaa gtagtaagtt
     3661 tataaag
//
LOCUS       DAOSID010000073         3623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_22509_length_3623_cov_16.056054, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000073 DAOSID010000000
VERSION     DAOSID010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22509_length_3623_cov_16.056054"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..798)
                     /locus_tag="PK674_03125"
     CDS             complement(85..798)
                     /locus_tag="PK674_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15556.1"
                     /translation="MLIAVDKPQGLSSFDIIRALKRELGEKKIGHSGTLDPMATGLLL
                     IGTDKDTKKLHELTGLDKKYITTIDFSKESDTWDLDYREYFEKYKIKDNGIIIQNNFI
                     SGPKIEDIKLKLGSIIPHGNLPLTPFSAKKKNGKKLYELAREGIQEIEYKTMKINGYE
                     ILEYNFPELILKLDVGSGTYIRSIGYRLGKEFGTGGILTKLRRISMGKYNLDEIELDK
                     KGTFFIKDGGEIDFKYKVV"
     gene            928..1095
                     /locus_tag="PK674_03130"
     CDS             928..1095
                     /locus_tag="PK674_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15557.1"
                     /translation="MLKPRLAGGIIKGCRGGYQPPVITVKNVRNEVLNIKIGVGVMGE
                     LEIVNHLEKLI"
     gene            1150..2457
                     /locus_tag="PK674_03135"
     CDS             1150..2457
                     /locus_tag="PK674_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="hemolysin family protein"
                     /protein_id="HOG15558.1"
                     /translation="MDPVLIIVFLVLFFLSAFFSGSETAFTSIPEYRVDAFIRQKKFG
                     AVELKKLKSNKDRLLITLLVGNNLVNIFTASLATSIAINIANSLGVAQSAAVGASTGV
                     VTLLLLLFGEIFPKTLANRYADKIALSVSKVYVVLQIILYPIIISIELLMKKLQKTKK
                     ERSMTDEELEAFIDMGKDMGVLEKGEHERIKNMLNFYEITCEEVMTPRVKIDAISCDL
                     SVDEAIEKLLQFSHTRILVHSGDVDNSEWVVTLKELFKVQRKGMGEKKLSELNLSTLI
                     KVPLTKPIHLLLDIFKRTRKHIAIVIDEYGGVAGLVSLEDVVEEVFGEIWDETDDEVD
                     SIRAYENGSYLFHSEVRVEEVLGRFDLEFDDIEMEESVFSGETIGYFIMSYLERFPKR
                     GDMISFVIQKYGDEEQDQFSKLNLKVMGVKKNIIGDVKAWVSD"
     gene            2656..3210
                     /locus_tag="PK674_03140"
     CDS             2656..3210
                     /locus_tag="PK674_03140"
                     /inference="COORDINATES: protein motif:HMM:NF020845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="elongation factor P"
                     /protein_id="HOG15559.1"
                     /translation="MVKIETSKVKKGMILQIDGKLFKVIDTSHTHMGRGGATDTFKVK
                     DIVSGKTNVFTYNAGTTLEQAEVQTNMAIFLYSAGDFYTFMENDTGEMYDLPKDKIDD
                     VIDYLKENLDVYLMIFEGEVIGVILPNAITYTIVSTVPGVKGNRAQSGTKPAIIETGL
                     EIQVPLHKNEGETVIVNTVTGEVN"
BASE COUNT         1195 a          468 c          672 g         1288 t
ORIGIN      
        1 taaaattttt atcaatttta cttttttgtt accgaattac taaacctata caggataacc
       61 atcgacttgc aaccttacaa acttttaaac tactttgtat ttgaaatcta tttctcatca
      121 gtcttttata aagaaagttc attttttatc cagttcaatt tcatctaaat tatattttca
      181 catacttatt cttctaagct ttgtaagaat tcatccagtt caaaactctt ttcaaagtcg
      241 gtatcatatt gatctaatgt aggttccgga tcatacatct agttttagaa tcaattcggg
      301 gaaattatac tccaatattt catatcaatt tattttcatg gttttgtatt ctatttcttg
      361 aattcattct ctggctaatt catataattt tttaccattt tttttctttg cgctaaatgg
      421 ggtaagtggt agatttcaat gaggaataat tgagcctaat ttaagtttta tgtcttcaat
      481 tttgggacct gaaataaaat tattttgtat gataattcaa ttatctttta ttttatattt
      541 ttcaaaatat tctcgataat ccaagtccca tgtgtctgat tcttttgaga agtctattgt
      601 ggtaatatat tttttgtcaa gcccagttag ttcatgtaat ttcttggtat ctttgtctgt
      661 tcatatcaaa agtagtccag tagccatggg atccaatgtc ccactgtgtc aaattttctt
      721 ttctccaagt tctcttttta gggcacggat tatatcgaag ctacttagtc actgtggttt
      781 gtctactgcg ataagcatgt gtactgagag gttataaggt taaaaagttc gcttcgctca
      841 cgataaaatg ataaaatgat aaaatgataa agtgataaag tgataaaatg ataaagtgat
      901 aaagtgataa aatgataaag tgataaagtg ttgaaacccc gcttagcggg gtgaattata
      961 aaatgatgta ggggcggtta tcaaccgccc gttattacag taaaaaatgt tcgtaatgaa
     1021 gtattaaata tcaaaatagg tgtaggggtg atgggagaat tggaaattgt aaatcatctt
     1081 gaaaaactta tatagaacat tataatactt tcaatacaaa attttaattc gtcatttgga
     1141 actacccata tggaccccgt cttaattatc gttttcttag tcttattctt tttgtcagca
     1201 tttttttctg gtagtgaaac cgcgtttaca tctattcctg agtatagagt agatgctttt
     1261 ataagacaaa aaaaattttg agctgttgaa ttaaaaaaac tcaaatccaa caaggatagg
     1321 ttgcttatta ccttattggt ttgaaataat ttagtaaata tttttacagc atctttggct
     1381 actagtattg caataaatat tgcaaattct ttatgagttg cccaaagtgc tgctgtatga
     1441 gcctctactt gagttgttac attattgcta cttttgtttt gagaaatatt cccaaaaaca
     1501 ttagccaatc gttacgcgga caaaatagca ttatctgttt ctaaagttta tgttgtttta
     1561 caaataattc tctatcctat cattatttca atagagcttt tgatgaaaaa acttcaaaaa
     1621 acaaaaaaag aacgctccat gactgatgaa gaattggagg catttataga tatgtgaaaa
     1681 gatatgtgag ttttggaaaa atgagaacat gaaaggataa aaaatatgtt aaatttttat
     1741 gagattactt gtgaagaagt catgactcct agagtcaaaa tagatgctat atcttgtgat
     1801 ttgtctgtag atgaagcaat agaaaaattg ttacaatttt cacatactcg tatattagtt
     1861 cacagttgag atgttgataa ttctgaatgg gtagttactc tcaaagaatt gtttaaagtg
     1921 caaagaaaag gaatgtgaga aaaaaagcta tctgaattaa atctgagtac cttgataaaa
     1981 gttcctctga caaaaccaat acatttgtta ttagatattt tcaaaagaac tcgcaagcat
     2041 atagctatag ttatagatga gtatggttga gtagcttgac tagtctcttt ggaagatgtt
     2101 gtagaagagg tgtttggtga aatttgggat gaaactgacg atgaagtcga ttctataaga
     2161 gcttatgaaa atggttcata tttgtttcat tctgaggtaa gagtggagga agttttggga
     2221 agatttgatt tggagtttga tgatatagaa atggaagaat ctgttttttc tggggaaact
     2281 atctgatact ttattatgag ttatttagaa agattcccga aaaggtgaga tatgataagt
     2341 tttgtgatac aaaaatattg agatgaggaa caagatcagt tttcgaaatt aaatttaaag
     2401 gtgatgtgag ttaaaaaaaa tataatctga gatgtaaaag cttgggtttc tgattaaaat
     2461 aacttgatat aaacttaaat aaatttgttg gaaccacttg ttttgattgt ttttttaaaa
     2521 aattctgttc tgcttgctat ttttggtaga atcattagta agtggatatg ttatttatca
     2581 taatgtgaaa attttttaag ttttttattc cgaaaagtcg gagtcgatga ctgttttatt
     2641 tctttatttt ttgtgatggt aaaaatagaa acatcaaagg tgaaaaaatg aatgattttg
     2701 caaatagatg gcaaactttt taaagtaata gatacttctc atactcatat gggtagagga
     2761 ggagctactg atacatttaa agtaaaagat attgtgagtt gaaagacaaa tgtttttact
     2821 tacaatgcag gaactacttt ggaacaagca gaagtccaga caaatatggc aatatttttg
     2881 tactctgcat gagattttta tacttttatg gaaaatgata catgagaaat gtatgatttg
     2941 ccaaaggata aaattgatga tgtaatagat tatttaaaag aaaatttaga tgtttatctt
     3001 atgatatttg agtgagaggt tatcggtgta attttaccta atgctattac ttatactatt
     3061 gtatctactg ttcctggggt aaaatgaaat agagctcagt ctggtacaaa accagcaatt
     3121 attgagacag gattggaaat acaagtccct ttacataaaa atgagtgaga gactgtgatt
     3181 gtaaatactg ttactggaga ggtgaattag tgagtagttc atatcaagtt agaaagcctg
     3241 ccccgccaca tagcccgccc cgccccgcag tattgcgggg ctgcggggcg gggcggggtt
     3301 cgtaaagttc ataaagttta taaggttttt aatggtggga gtaaagttcc ccttaataag
     3361 ggggattaag ggggatctaa cccccaacta ccccgcaata ctgcgggggc ccagcttccc
     3421 caagggaaga atttttttaa taaagttcgt aaagttaata gatgaaataa tagtggtggt
     3481 atatgtgaaa acgacaaaat aaaattattt ttgattttgt ttgtgtcgtc tttctagtat
     3541 aagcatagcg ctaactgtat cttcagccac attttttttg aaattggaga ctatctcgcc
     3601 ggattggaca cttgtataat ctt
//
LOCUS       DAOSID010000074         3576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_22874_length_3576_cov_15.019597, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000074 DAOSID010000000
VERSION     DAOSID010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22874_length_3576_cov_15.019597"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..448)
                     /locus_tag="PK674_03145"
     CDS             complement(107..448)
                     /locus_tag="PK674_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOG15560.1"
                     /translation="MREIIKIPYDIYESPNEMVVVMPLGGVEKETLDISIKDYRLIVK
                     GLRKKVLLKDDLIPLREDCYWGKIEQVLDLPPQAYFDKIHSKLSSDNVLQIIIPKSIV
                     PEKIQLEVERH"
     gene            complement(461..1042)
                     /locus_tag="PK674_03150"
     CDS             complement(461..1042)
                     /locus_tag="PK674_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15561.1"
                     /translation="MGILSTFEFGFQKRIANESIERYGKENVIVIRKSKFFLWTKVLI
                     PFLFWTTILVVVLVLLIKHISTSWLLWSLIVLSCFLWIAPNIKTLQYFLDYEMDFIVV
                     NPRSFIRYDQDGLFQRVSKTIDLKKIRSISVRKRGFWNSIFNNGSLLVVSEGGEAEQD
                     EKLRAGEINFRNLYNPEAYSKQINEFLSKIFNE"
     gene            complement(1068..1667)
                     /gene="lepB"
                     /locus_tag="PK674_03155"
     CDS             complement(1068..1667)
                     /gene="lepB"
                     /locus_tag="PK674_03155"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="signal peptidase I"
                     /protein_id="HOG15562.1"
                     /translation="MGFSDNVRVKFKNILLDIFDMFSFFVFVLGAVLFVRFFIANPYT
                     VIGASMAPTFHENDFIIVDKISKRFDVLSRGDVIVFVPEGKTTPYIKRIIGLPGEMVK
                     IQDGLIFICDISGNNCEQLMEQYLPEDLQTKTRCGKTEFQIENGYFVLGDNRGFSTDS
                     LCCFGLGCYDGANYEVGNQDIIGRVLFRVFPSFATSFNE"
     gene            complement(1812..2204)
                     /gene="ssb"
                     /locus_tag="PK674_03160"
     CDS             complement(1812..2204)
                     /gene="ssb"
                     /locus_tag="PK674_03160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOG15563.1"
                     /translation="MNKVQLIGRSTSDLEVKTIESSGTAVVNFTIVTNRKFKNRDENM
                     VEEAEYHRCVAYGKGAEILGQYVTKGKRIYVEGRLRTRRWQDTEGKDRYMTEIIVDNF
                     IFLDSKAPETAGEIEEPSADIVDEEVPF"
     gene            2846..3097
                     /locus_tag="PK674_03165"
     CDS             2846..3097
                     /locus_tag="PK674_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOG15564.1"
                     /translation="MKYTVYIIKSINHNYTYTGMTNNLERRLNEHNLGNTPSNKKYAP
                     FKLIYHEIVENSKIARQREKYLKGGNGRNRLKKQLETTQ"
     gene            3373..3456
                     /locus_tag="PK674_03170"
     tRNA            3373..3456
                     /locus_tag="PK674_03170"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3407..3409,aa:Leu,seq:caa)
BASE COUNT         1255 a          668 c          458 g         1195 t
ORIGIN      
        1 tctaaatcaa tctttttttt agtcgttttt ttaacagcca tttttatttt aatataataa
       61 taaagagaat atttaaaaac aattagttaa catcaacaaa taagctttaa tgtctctcta
      121 cttccaattg aattttttct ggaacaatag attttggtat tattatttgc aaaacattat
      181 cagatgaaag cttactatgt attttatcaa aatatgcctg tgggggcaga tccaaaacct
      241 gttcaatttt tccccaataa cagtcctctc tcaatgggat taagtcatcc tttaataaaa
      301 ccttttttct aagtcatttt acgatcagtc tataatcttt aatactaata tcaagagttt
      361 ccttttctac tcatcacaaa ggcataacca ccaccatttc atttggagat tcatatatat
      421 cataaggaat ttttattatt tctctcatta ttttgtatag ttattcatta aatattttgg
      481 ataaaaactc attaatctgt ttactataag cttctggatt ataaagattg cgaaagttga
      541 tttctcaagc tcttagtttt tcatcttgtt ctgcttctca tcactctgaa acaactaata
      601 gtgatcaatt attaaatata ctattccaaa agcctctttt tctaacagat attgacctaa
      661 ttttttttag atctatagtt ttggaaaccc tttgaaacaa accatcttga tcgtatctta
      721 taaatgatct gggattcacc actataaagt ccatttcata gtccaaaaaa tattgtaatg
      781 tctttatgtt tggggcaatc cacaaaaaac atgataatac gataagtgac cataacaacc
      841 aagaagtgga aatgtgtttg ataagtagta ccagcaccac aaccaaaatt gttgtccaaa
      901 acaaaaatgg aatcaatact tttgtccaca aaaaaaactt acttttccta attacaatca
      961 cattttcttt tcaatatctt tctattgatt catttgctat tcttttttga aatccaaact
     1021 caaaagtaga caatatcccc attttatatg ccagttatta cataaaacta ctcattaaaa
     1081 ctagtagcga aactgggaaa taccctaaac aaaaccctac ctattatatc ttgattccca
     1141 acctcatagt ttgctccatc ataacacccc aaaccaaagc aacacaaaga gtctgtacta
     1201 aatcacctat tatctcacaa aacaaaatat ccattttcta tttgaaattc tgttttccca
     1261 catctagttt tagtttgtaa atcttctggt aaatactgtt ccattaattg ttcacagtta
     1321 tttcatgaaa tatcacaaat aaagataagt caatcttgta ttttaaccat ctcccctggc
     1381 aatcaaatta ttcttttgat atacggtgtt gtttttcatt ctggaacaaa aactattaca
     1441 tctcacctac ttaatacatc aaatcttttg gatattttat ctactataat aaaatcattt
     1501 tcatgaaaag tgggagccat acttgctcaa atcacagtat acggatttgc tataaaaaac
     1561 ctcacaaata aaacagcacc caatacaaat acaaaaaatg aaaacatatc aaaaatatcc
     1621 aacaagatat tcttaaactt tactctaaca ttatccgaaa atcacatcgt ccagtaaatt
     1681 tatttaatta aagtctaaat tcatcaacaa aaacaagata caacaaaaac catcacagtt
     1741 tttatataca taaacaaaaa atccgtacat aaaaacaaat aaccttcact tttattttta
     1801 tttcattcaa attagaatgg cacttcttca tcaacgatat ctgctgaagg ttcttctatt
     1861 tcaccagcag tttcaggagc cttagaatcc aaaaagataa agttgtccac aataatctct
     1921 gtcatgtacc tgtcttttcc ttctgtgtct tgccatcttc tagttctcaa ccttcactca
     1981 acataaattc tctttcattt tgtaacatac tgtccaagaa tctccgctca tttcccataa
     2041 gcaacacatc tgtgatactc ggcttcttca accatgtttt cgtccctgtt tttgaacttt
     2101 ctgtttgtaa caatggtaaa gtttacaaca gcagtaccag aactttcgat ggtctttact
     2161 tctaaatcac tagtagatct tccaattaac tgtactttat tcaaatccat tttctaattt
     2221 tacattctaa aaatttttgg tgaatagttc acctcaacat aactaataat taaaaattat
     2281 taaaaatcaa ttgattttta actctatatt tttaaaagtt ctttcgctac atcaaagtgg
     2341 tttataaagc tagcctacgg cgagtttatc aagttacaag aagttatcga gttacaagtt
     2401 cataaagtta aaagttataa agtttgtaaa attacaattt gaaagtttat aaactttatt
     2461 aactttacga acttgataaa cttttaactt aattatgccc agatgatgct ccaagattgg
     2521 gttgatttta cgagcgaagc gagtaaaaat caaggacaag attgaaagtc ccgctgaata
     2581 gcgggagaat tgttccaaga ttgttgataa accacgcata gcgtggtaaa gcacgcgtag
     2641 cgtggtaaaa ctcaaaatct gatacacaca attaaaaaac attatgccca gatgatggaa
     2701 ttggtagatt tatccagcta agctgggcat gctatccacg cgtagcgtgg taaaactcaa
     2761 aatctggtgg cttcacggcc atgtgggttc aactcaatct ttgaaaaaga taaataagaa
     2821 acattgatat attaacaata actagatgaa atacacagta tacataataa aaagcataaa
     2881 tcataattac acttatacat gaatgacaaa taatcttgaa agaagattga acgagcataa
     2941 tttatgaaac accccatcaa acaaaaaata tgcaccattt aaattaatat accacgaaat
     3001 tgttgaaaat tcaaaaattg ctagacaaag agagaaatat ttgaaatgat gaaattgaag
     3061 aaatcggttg aaaaaacaac tagaaacaac acaataaaag aaatgatgga attggtagat
     3121 ttacccagct atgctgggtt tatccagctc tgctgggttt atccagctct gctgggttta
     3181 tcccgctctg ctgggtttat ccagctctgc tgggtttatc ccgctctgct gggtttatcc
     3241 cgctctgctg ggtttaccca gctgtgctgg gtttacccag ctaagctggg catgctatcc
     3301 acgcgtagcg cggtaaacca ctcgtagcgt ggtaaaactc aaaatctgat acacacaatt
     3361 aaaaaacatt atgcccagat gatggaattg gtagacatgc tagactcaaa atctggtggc
     3421 ttcacggcca tgtgggttca actcccactc tgggcaaatt attaaaaaat caatctgtac
     3481 ttaaatgttc agattttttt tatttttttt aatcaaaaaa tttcaatttt aatcaatcca
     3541 attttcaaaa aaacttgttt tttactgaat attgaa
//
LOCUS       DAOSID010000075         3570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_22922_length_3570_cov_11.832432, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000075 DAOSID010000000
VERSION     DAOSID010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22922_length_3570_cov_11.832432"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PK674_03175"
     CDS             <1..453
                     /locus_tag="PK674_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15565.1"
                     /translation="EQDEYAVHTPQSTKIITKSDKRQIKKNFIKFTIGVVLLLMSWSY
                     IQKHPAEKVSVFSGFEVLIQKVEVFFQNTFGHNGELLERKYSMEKYYKELVKMAENNK
                     CIDTNILQEIEDVYKNLKKEKLDGLEQILPEYTKKAYEYDNIVKNNDC"
     gene            complement(893..2098)
                     /locus_tag="PK674_03180"
     CDS             complement(893..2098)
                     /locus_tag="PK674_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="site-2 protease family protein"
                     /protein_id="HOG15566.1"
                     /translation="MIRSILLGILMFMVLVILHELGHFITARRSGVKVVEFGIGIPPK
                     ICKLATDKKGTEYTLNLIPLGGFCRLKGDDPKDAKDFFAKDGFISAKFWNKLLILAGG
                     VLMNLIIAWIIFTTVFTIGTKPLSVLPENAIKGESHSLLMPTYSYLEDQGFIVGEKSD
                     IPLLIDMIAEGSIAESLGLQTGDIITNINNISVNARNIGIVLKENIGNKIDISYKREE
                     QENTVNGKCEEDSCILGIAFITSGNLQVNDIKYPIQQAMGMGLKEIGEQVKLTFSALG
                     KLGKNLFSFDRSKIKGSLSGLTGPVGVIKFGGDLLEVGGRISYLAFAGLISLALAIFN
                     ILPIPALDGGRIIGVLLQKLGRIKPDKYFIIEGYINTVFFVLLMALGVYIIFKDLSVF
                     RGLKIPFIG"
     gene            complement(2408..3103)
                     /locus_tag="PK674_03185"
     CDS             complement(2408..3103)
                     /locus_tag="PK674_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15567.1"
                     /translation="MTGKGNQLKFSFIDETPIKKGAQKGDKPLTPKERGIFNEFVRGN
                     FKHLHALTNRSEEEIYALLLKDNRTLPELKNELTKKIEYYITHISDKELINHLLKKEN
                     NGKYIVDIEIKDEEGFEKLFHSLPLIKKVAICLRFDNKPPFELKKILTEESLQDLDLA
                     DDAILFFDPLLINNEKYFEYLLKNKKINSLLYEKIGFRKMLRLIYKNGYKAPIINTYL
                     KLLFNSKDTEHTN"
BASE COUNT         1245 a          489 c          520 g         1316 t
ORIGIN      
        1 gaacaagatg agtatgcagt acatacacct caatctacaa aaattattac aaaaagtgat
       61 aaaagacaaa taaagaaaaa ctttataaaa tttactattt gagtagtttt gcttttgatg
      121 tcttggtctt atattcaaaa acatccagca gaaaaagtta gtgttttttc tggatttgag
      181 gttttaatcc aaaaagtaga agtctttttt caaaatacat ttggtcataa ttgagaatta
      241 ttagaaagaa aatatagcat ggaaaaatat tataaggaac ttgtaaaaat ggctgaaaat
      301 aataaatgca ttgatactaa tattttacaa gaaatagaag atgtctataa aaatttaaaa
      361 aaagaaaaac ttgattgatt agagcaaatt ttgcctgaat atactaaaaa agcttatgaa
      421 tatgataata ttgtcaaaaa taatgattgt taaaatttgt tcaaaacaat aaaaataact
      481 ttctcgcaag gtagaacaat tgaaatgaca gtaaatggat tcttcgctac gctcagaatg
      541 acggtgctca gtcatattga gcccataggg cgaaatatcc attaacagaa agaggattat
      601 gagcaatgga ttcttcgctt tgctcagaat gacgaggtgt tcgctcagaa tgacaacagt
      661 attatgtcat ctcgagtgaa tattgttgcg gatttgtcat ctcgagtcgt tattgttgcg
      721 agagatctaa aaaaacagat tcctcacaag ctagaacatt tcagaattac agaaaaaaga
      781 tttcttgcat ttagaacacc ttgaaacgac acaggggaaa aatgtttcga aataataatg
      841 acgaaaacac tcaacattaa gtttatgttt tttagtttat tattttttta atttatccta
      901 tgaatgggat ttttaatcat cgaaaaactg ataaatcttt gaatataatg tagactcata
      961 gtgccatcaa tagtacgaaa aaaacagtat ttatgtatca ttctataata aaatatttgt
     1021 ctggttttat ccttcctaat ttttgtagca aaacaccaat tattcttcct caatctagtg
     1081 caggaattgg caatatgttg aatatggcta aggctaaaga tattaatcct gcaaatgcaa
     1141 gataagaaat tcgtcatcaa acctctagta aatctcatcc aaattttatt actcatactg
     1201 gtcaagttaa gccactcaaa gatcatttta tttttgatct atcaaatgaa aataagtttt
     1261 ttcataattt tcataatgct gaaaaagtga gttttacttg ttctcaaatt tctttgagtc
     1321 ccattcacat tgcttgttgt attggatatt ttatatcatt tacttgcaaa ttaccagatg
     1381 taataaatgc tatccccaat atacaactat cttcttcgca tttcccgttt actgtatttt
     1441 cttgttcttc tcttttgtag gaaatatcta ttttgtttca aatattttct tttaatacta
     1501 ttccaatatt tcgagcattg acacttatgt tatttatatt tgtgataata tctcccgttt
     1561 gaagtcatag tgattctgca atcgatcatt ctgctatcat atctattagt aaaggaatat
     1621 ctgatttttc tcaaacaata aatcattggt cttctaaata tgaataagtt ggcatcaata
     1681 aactatgtga ttctcatttt atggcatttt caggcaaaac agaaagtggt tttgttcata
     1741 ttgtaaacac ggtagtaaag attatccatg ctataattag gttcatcaaa actcagcctg
     1801 ctaaaattaa taatttattc caaaatttag cagagataaa tcagtctttt gcaaaaaaat
     1861 cttttgcatc ttttgggtca tctcatttta atctacaaaa tcatcatagg ggaattaaat
     1921 ttagagtata ttctgttcat tttttatcag tagcaagttt gcatattttt ggagggattc
     1981 atattccaaa ttccacaacc ttcactccag atcttctagc tgtaataaaa tgtcagagtt
     2041 catgtaaaat tactaaaacc ataaacataa gtattcataa taaaattgat cgaatcatgt
     2101 ttttcttgtt gtaaaatagt aaataataat ttttagagga tactaaattg ttgataaaaa
     2161 acaagatgaa agtcaagttt tttggaaaaa agtttaaaaa taaaagacaa tgctaactaa
     2221 gaaggggtaa ttaaagttta tctaattctt tgtcagggat tctactcggt attttttcca
     2281 aaggagaaaa agtatgtgat aaaaattctt ataagttttt cattttttgg gatatttttc
     2341 tatttctttt aatggaacct tttacaacaa aattaaataa accctagata ttctaataaa
     2401 gtttctttta atttgtgtgt tctgtgtctt ttgagttaaa aagtaattta agataggtgt
     2461 tgataatagg agctttatat ccatttttgt atattaatct taacattttt ctaaacccta
     2521 ttttttcata aagaagcgaa ttaattttct tgttttttaa aagatattcg aaatactttt
     2581 cgttatttat taataatgga tcgaaaaaaa gaatagcatc atcggcaaga tctaaatctt
     2641 gtaaagattc ttcagttaaa atctttttca gttcaaaagg aggcttatta tcaaatctaa
     2701 gacaaatagc tacttttttg ataagaggaa gagaatggaa aagtttttca aatcattctt
     2761 catcttttat ttcaatatca acaatatatt ttccattgtt ttcttttttt aaaagatggt
     2821 ttattaattc cttatctgaa atatgagtta tataatattc tattttcttt gtgagttcat
     2881 tcttgagttc gggtagtgtt ctgttatctt taagtaaaag tgcataaatt tcttcctcag
     2941 acctgtttgt aagggcatgg agatgtttga agtttcacct aacgaactca ttaaaaattc
     3001 cccgttcttt tggtgttaaa ggtttgtctc atttttgagc tcacttttta ataggggttt
     3061 catctataaa agagaattta agttgatttc cttttcaagt cattttcttt tttttagcta
     3121 ataaaaaata tataacatat attgtatata tatatttgaa gtaaaaagtc aaactaagtt
     3181 aacaagttca tcaaattcgt aaagcctgcc ccgccccgcc ctgcagcccc gcaatactgc
     3241 ggggcggggc tgcggggcgt ggcgtggttc ataataaagt tcatcaagta ttaataactt
     3301 tataaacttt taacttgtaa ctttataact tgtaacttat tttgtgataa acaatttgaa
     3361 attttgtcgg gaaactgtat tgtgtttatg tttatataat attttttctt atgaaattaa
     3421 aaatctacaa cactatgtct cgtcaaaaag aggaatttgt accacttatg gaagatccaa
     3481 attacaattg accaaaaaaa gattttgttt gaatttattc gtgttgacct actgtttatc
     3541 gagatccaag tatatgaaat tatagagcaa
//
LOCUS       DAOSID010000076         3558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_23004_length_3558_cov_11.605481, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000076 DAOSID010000000
VERSION     DAOSID010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23004_length_3558_cov_11.605481"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            508..1983
                     /locus_tag="PK674_03190"
     CDS             508..1983
                     /locus_tag="PK674_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="MFS transporter"
                     /protein_id="HOG15568.1"
                     /translation="MTKKNKKDFKKTTAQKAIKLPIKKTDKIKTTSKKKKDHTNVTKF
                     LSSTRDLLFQTKEQELKNGLFSVMKRRFFSIPVSVRFISLSLFLFVFGWGLGGDIFFS
                     IYVERIVTAVMLVSLIGMLGPIIKLLLVLQVGSLNDNTNRKYVVFLGKFFYIFTSIFY
                     FLAGILKSPGLLIIAVLLNGVGSVLVYVTYEGYIRATTESDKSESSWGLYFSSWHIAR
                     VIGAIIASFLVKFLALPYLFLFIGIFSILSLITDQKIPILDKEKVKKLFGKQSFLRHT
                     LKKIFSKKPVKNAIIFIKESPRRFIYTLGYEGLYNVLEYIGFLFIPLIAIRNSFSLSQ
                     IALIFALMKLPFVTNFFVVSFSEKFNKKLFIAVVLIFLSFLYGMLGFEMSFGSIMIIT
                     FGISIGLSLIRPLVSALISESSVKENAGTITGIQQFVGQVGKIVGSLGFGILGALFGM
                     YSAFFLVGLSLFILATWGLAKRFNRQKAVQKRLLAKQNMKK"
     gene            complement(2347..>3558)
                     /gene="glyA"
                     /locus_tag="PK674_03195"
     CDS             complement(2347..>3558)
                     /gene="glyA"
                     /locus_tag="PK674_03195"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOG15569.1"
                     /translation="ENYQSEAVLQAQSSVFANKYSEGFPGKRYYGGQANTDIVEQIAI
                     DRAKKVFHADHANVQALSGATANICVYAALMEPGDTILGMDLSHGGHLTHGAPVTFLS
                     KVFNFIRYKTLPNGEIDFDQVKKLAHQHKPKIILAGFSAYPRELDYDKFAEIAKEVGA
                     VAFADVSHIGGFIATGVLKNPLDHGFHVMMTTTHKSLRGPRGALILSKGIVSNPLKKP
                     ENTVENIPTRIDRAVFPGIQGGPHINTISAIAVALLEAQTDKFKEYAIQALKNSKVMA
                     EEFINRGYKLVTGGTDNHMIVLDFSNTPVGNGKNAEHILDLVGISTSKSTIPDDPNPP
                     FRPSGLRIGTPAMTTRGVKEDDVRKIVDFIDQALKLIPLMEEVDFHSKIEQLRKQVID
                     FSKQFPVPGIS"
BASE COUNT         1130 a          519 c          596 g         1313 t
ORIGIN      
        1 tcttatattt taaatcatcc cccaaccccc tctactttta attctaaatc acccctatcc
       61 cctcttatat tttaaatcat cccccaaccc cctctacttt taattctaaa tcacccctat
      121 cccctcttat attttaaatc atcccccaac cccctctact tttaattcta aatcaccccc
      181 ttaatccccc tctttgaaaa agagggggaa atagtaggtg aaaactggga aagcccctct
      241 ttttcaaaga ggggtttggg gtgatttaga ggaagaagag gggttttagg gtgattgaag
      301 accagaggga gtcaggggtg atttatgttc aaaaaaggga gtcaggggtg atttagagga
      361 agaagagggg ttttggaagg aatttaaaag agaaaatgga gatcgaaatg ctacattttt
      421 tatatttaaa aataaatatg ctccattttt tgacaaattt aaaaaaatta ttataatagt
      481 gatacattta ttattaaaaa tattttcatg actaaaaaaa acaaaaaaga ttttaaaaaa
      541 acaacagctc aaaaagctat taaactcccg atcaaaaaga ctgataaaat taaaacgaca
      601 agtaagaaaa agaaagatca cactaatgtt actaagtttt tgagcagtac tagggattta
      661 ttgttccaga caaaagagca agaacttaaa aactgacttt tttccgttat gaaaagaagg
      721 tttttttcta tcccagtatc tgtaagattt atttctttat cactattctt atttgttttt
      781 ggttggtgat tgtgatgaga tatttttttc tcaatatatg tagaaagaat tgtaacggct
      841 gttatgcttg tttccttaat atgaatgttg ggaccaatta ttaaattatt attagtatta
      901 caagtatgaa gtttgaacga caatacaaac agaaaatatg ttgtgttttt gggaaaattc
      961 ttctatattt tcactagtat attttatttt ttggctggaa tactaaaaag tccaggttta
     1021 ttgataatcg ccgtattatt gaattgagtt ggtagtgttt tggtttatgt aacttatgaa
     1081 tgatatatcc gtgctacaac agaatcggac aaatcggaaa gttcttggtg attatatttc
     1141 tctagttggc acatagctcg ggtaattgga gcgattattg catcattttt ggtgaaattt
     1201 ttagccttac cttatctgtt tttatttata ggtatatttt cgattctatc tttgattaca
     1261 gatcaaaaaa taccgatatt ggataaagaa aaagtaaaga aattgttttg aaaacagagt
     1321 tttttgaggc atacactcaa aaaaatcttt tcaaaaaaac cggtaaaaaa tgctattatt
     1381 tttataaaag aaagtccaag gagatttatt tacactttat gatatgaatg attgtataat
     1441 gtattggaat atataggatt cttgtttata cctttgattg cgattaggaa ctccttttca
     1501 cttagtcaaa tagcgttaat ctttgctttg atgaaattac cttttgttac taactttttt
     1561 gtagtttctt ttagcgaaaa atttaataaa aaattattta ttgccgtagt attgatattt
     1621 ttatcgttct tatattgaat gttatgattt gaaatgtcct ttggatcaat aatgatcatt
     1681 acattcggta tatcaatatg attatcactt attagaccgc tggtatcagc tttgattagt
     1741 gagtcttctg taaaagaaaa tgcttgaact ataactggaa ttcagcagtt tgtatgacaa
     1801 gtaggaaaaa tagttggaag tttgtgattt ggtattttag gtgccctttt tggtatgtat
     1861 agtgccttct ttctagttgg attgagtttg tttatactag caacttgggg cttggcaaaa
     1921 agatttaaca gacaaaaagc tgttcaaaaa agattattag caaaacaaaa tatgaaaaaa
     1981 taaagaaata cagtcaaata aaccgagtaa atttactcgg ttttttatgg tttaacaata
     2041 aattttgttt attatttttg gggcaagtgt ttgtattttt gagttaatcg gttttacata
     2101 atttagttta taaaatttga tatttttttt attttcaaca aaacattgta ttttttagga
     2161 gaaagtaaaa ctaatttgaa aaaaaaatta taagttgaaa gttgatagtt gtcccccttg
     2221 ctcgttacgg agaactccgt agataagggg gattaagggg gattaaaccc acttgtggcc
     2281 aaatcccccc tacccccctt aaaaaggggg gcatttgaga actttcaaac ttgacaaact
     2341 ttctaactaa cttatgccag gtactggaaa ttgttttgaa aagtctatta cttgttttct
     2401 cagttgttct atttttgagt gaaaatctac ttcttccatc aaaggtatca actttaatgc
     2461 ttgatctata aaatctacta ttttacgaac atcatcttct tttactcatc tggtagtcat
     2521 agctggtgtt catattctaa gtcctgatgg tctgaatggt ggatttgggt catctggtat
     2581 tgtggattta gaagtagaga tccctactaa atccaaaata tgctcagcat tttttcagtt
     2641 tcccacggga gtatttgaaa aatctagcac aatcatgtgg ttgtctgttc atcatgttac
     2701 aagtttatat catctattga taaactcctc tgccattact tttgaatttt tgagggcttg
     2761 tatcgcatat tctttgaact tatctgtttg tgcttctaat agtgcaacag caatcgctga
     2821 aatagtattt atatgcggtc atcattgtat tcacgggaat actgctctat ctatcctagt
     2881 tggtatattt tctacagtat tttctggttt ttttaatggg ttacttacta ttcatttact
     2941 aagaatcaaa gctcaccttg gtcatctcaa agatttatgg gttgtggtca tcattacatg
     3001 aaagccatga tctaatgggt tttttaatac tcatgttgct ataaatcatc atatatgaga
     3061 tacatcagca aaagctacag cacctacctc tttagcaatt tcagcaaatt tgtcgtaatc
     3121 taattctcta ggataggctg aaaatcaagc taatataatt tttggtttat gttgatgtgc
     3181 tagctttttg acttgatcaa aatctatttc tcaatttgga agagttttgt atctaataaa
     3241 attgaatact ttggataaaa aagtgactgg agctcaatga gttagatgtc atcaatgtga
     3301 taaatccatt caaagtattg tatctcatgg ctccataagt gctgcatata cacatatatt
     3361 tgcggtagca ccagaaagtg cttgtacatt ggcatgatct gcatgaaaaa ctttttttgc
     3421 tctatctata gctatttgtt ctacaatatc cgtatttgct tgtcatcagt aatatctttt
     3481 tcatggaaat cattcagaat acttatttgc aaaaacactt gattgtgctt gtagtactgc
     3541 ttctgattga tagttttc
//
LOCUS       DAOSID010000077         3496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_23474_length_3496_cov_16.586748, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000077 DAOSID010000000
VERSION     DAOSID010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23474_length_3496_cov_16.586748"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="PK674_03200"
     CDS             complement(<1..1134)
                     /locus_tag="PK674_03200"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="ribulose-bisphosphate carboxylase"
                     /protein_id="HOG15570.1"
                     /translation="MTIKEMFKTLNSHQQAYVNLELPDPKNGEYMLACFHMLPGKNLN
                     MLQAACEVASESSTGTNFAVMTETPYSRSMNALVYQVDLKKELVWIAYPWRLFDRKGN
                     VQNILTYIAGNVFGMKEVKALKLLDVRFPPAMMEQYDGPSYTLQDMRDYLGVYDRPIL
                     GTIVKPKMGLTSAEYAEVCYDFWVGGGDFVKNDEPQADQDFSPYDKMVRNVKEAMDKA
                     VKETGKKKVHSFNVSAADFDTMIERCEMIVNSGMEPGSYAFLIDGTTAGWTAVQTLRR
                     RYPNVFIHFHRAGHGAFTRPENPIGFSVLILSKFARLAGASGIHTGTAGVGKMAGTPG
                     EDITVAENIYFYLADGHIFDQNWGLIPEHDEDLIEKIQMDNQFV"
     gene            complement(1667..3223)
                     /locus_tag="PK674_03205"
     CDS             complement(1667..3223)
                     /locus_tag="PK674_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15571.1"
                     /translation="MKNKTFSLKAQLINVTTGNDFQIWLNESQAFKFGITAVDNVILS
                     YEKDGKHKRLSVNVDLTNDFLESGEVGITQDIFEKHDIKTGDIVSISTITKNPLSLQA
                     IRKKLFGKKLNKNEIKALMRDMTDGKISDLLVAYYAACSFAYSSDKEELYWTAKYSAE
                     MGDMIKFKYDTAVKYSIGGVPGNETTMIIVPILGSLGMSSPKTFSRAITSPAATGECV
                     EVLMDTEFSVSEMKKLVTKNLSCLANGEFLKFAPANDKIIKVSYPLYMEAYSKVAISI
                     MAKIYAGGTKFCLIDLPVGKNGKIKDQKTADRIAGHFKYIGEKLGMKVYVEISDANHP
                     VGKGIGAVLQTREVLRILQNKKCISKDIKEKSIKLSADLIRLVGLAKGKKAIELATKQ
                     LESGAAWEYMKKLITAQNRIRNRKPDYKKVSYLGIVDSEDLPLAKFSEKIIAKKAGKI
                     KSLNVQLIKDIARLFGAPFDKQAGIYFYKQHGNLVKKGDVLCELYSNNQEDLQRGLEA
                     IKKEKLFFYC"
BASE COUNT         1142 a          704 c          440 g         1210 t
ORIGIN      
        1 tacaaattga ttatccattt gtattttttc tatcaaatct tcatcatgtt ccggtataag
       61 tccccaattt tggtcaaaaa tatgtccatc cgccaaataa aaatagatat tttctgcaac
      121 tgttatatct tctccaggag ttcatgccat tttaccaact catgcagtac cagtatgaat
      181 cccagatgct ccagccaatc tagcaaattt agacaaaatt aatacggaaa aaccaatagg
      241 attttctggc ctagtaaaag caccatgtcc agctctatga aaatgaataa atacatttgg
      301 atatcttctt ctaagagttt gaactgctgt ccatcctgct gtagttccat caatcaaaaa
      361 tgcataagaa cctggctcca taccagaatt tacaatcatt tcacatcttt cgatcattgt
      421 atcaaaatcc gcagcagaaa cattaaatga gtgtaccttt ttctttcccg tttcttttac
      481 agctttatcc atagcttcct tcacatttct aaccatttta tcataagggg agaaatcttg
      541 atcagcttgt ggttcatcat tttttacaaa atctcatcat catacccaaa aatcataaca
      601 tacttctgca tactcagcag atgttaatca cattttaggc tttacaatag taccaagaat
      661 cggtctatca taaactcata aataatctct catatcttgt aaagtataac taggtccatc
      721 gtattgctcc atcattgcag gtggaaatcg cacatccaga agttttagtg cttttacttc
      781 tttcatacca aaaacattac ctgcgatata agtaagaatg ttttgaacat ttcactttct
      841 gtcaaacaat ctccaaggat aagcaatcca aactaattct ttttttagat caacttgata
      901 aacaagagca ttcatacttc ttgagtacgg tgtttctgtc atcacagcaa agtttgttca
      961 tgtacttgat tctgaagcta cttcacatgc tgcttgcaac atattcaaat ttttaccagg
     1021 gagcatatgg aagcatgcca acatatattc cccattcttt ggatccggta actctaaatt
     1081 cacataagct tgttgatgcg aatttaaagt cttaaacatt tctttaatag tcattttgtt
     1141 tatatataaa taaattaaaa actaattttc atgctaagaa gtaattctta tcactactca
     1201 cattctatca caagaaaaca aagttttcaa atttcttgct tagtaatata attatactca
     1261 aaaaaacaaa aaaaatcaaa aaaaccgtag ttaaaatacg attttgatat acatttttta
     1321 tttttaatat ataatatcat taattatcat cacccgagtg aaacataaac aatcaacact
     1381 aataaagaac agttattttt cacaaaccat catcgcaaaa cctatcattt cgaacatagt
     1441 caaaaaatct cctttattat cttctgtcgt ggcaagctac tgaacaaacc agaacaattt
     1501 ccatagggtt ctcactcaaa caaaattcct ccctaggggg agggtgttct cccccgcagt
     1561 actgcggggg taggtagggg ttaaatagcc ctaacaaact ccaattaccc ccaactattg
     1621 aatttacaaa tccccctcaa tcccccttaa taagagggat gcccgcctag caataaaaga
     1681 aaagtttctc tttttttata gcttccaatc atctttgaag atcctcttga ttgtttgaat
     1741 acaattcaca caaaacatct cactttttca ccaaatttca atgttgttta taaaaataaa
     1801 ttcaagcttg tttatcaaac ggtgctcaaa acaatctagc tatatctttt attaactgaa
     1861 cattgagtga ttttattttt caagcttttt ttgctattat tttttctgaa aattttgcca
     1921 aaggcaaatc ttctgaatct actattccca gataagaaac ttttttataa tcaggtttcc
     1981 tattgcgaat acgattttgt gctgtaatta attttttcat atattcccaa gcagctccag
     2041 attccaattg ttttgtagcc aattcaatag ctttttttca tttagccaat caaacaagac
     2101 gaatcaaatc agcggataac ttaattgatt tctctttgat atcttttgaa atacattttt
     2161 tattttgaag gattctgaga acttctcttg tttgcaaaac tgctcatatc cccttaccca
     2221 caggatgatt cgcatcagat atttccacat aaactttcat cccaagtttt tctcaaatat
     2281 atttgaaatg accagcaatt ctatctgcag ttttttgatc ttttattttt caatttttac
     2341 caacaggcaa atctattaaa caaaacttcg ttcatcaggc ataaattttt gccataatac
     2401 taattgctac cttggaatat gcttccatat acaatgggta tgaaaccttt attattttat
     2461 catttgctgg agcaaatttc aaaaattctc cattagccaa acaagataaa tttttagtaa
     2521 ccaatttttt catttcactg accgaaaatt cggtatccat cagcacttct acacactctc
     2581 aagttgcagc aggagaagtt atagctctag aaaaagtttt tggactagac ataccaaggg
     2641 aacccaaaat aggcacaata atcatagttg tttcattccc aggtactcaa ccaatagaat
     2701 attttacagc agtatcatac ttaaatttaa tcatatctca catttcagca gaatactttg
     2761 ctgtccaata aagttcctct ttgtcactag aataagcaaa actacaagcc gcataataag
     2821 ccacaagtaa atcactaatt ttcccatctg tcatatctct cattaatgct ttaatttcat
     2881 ttttatttag tttttttcca aaaagttttt tccttatagc ttgcaatgat agtggatttt
     2941 tagtaattgt agaaatagaa actatgtctc aagtttttat atcatgtttt tcaaaaatat
     3001 cttgggtgat tcaaacctct caagactcca aaaaatcatt agtcaaatca acatttacag
     3061 ataatctctt atgtttgcca tctttttcat aagataaaat tacattatct actgctgtaa
     3121 taccaaactt aaaagcctgc gattcattta accaaatctg aaaatcattt ccagttgtaa
     3181 catttattag ttgtgctttc aaagaaaagg ttttattttt cattcttata ataataagtt
     3241 tgtaaagttc atagccccct tcaactttcc aaactccgat tttcggggtg aaaggaagaa
     3301 ttttgtgggg aaagttcctc cccttgggga ggttgttctc ccccgcagta ctgcggggat
     3361 aggtgggggt tttacttcca accaccccca actacattag aacagcttcc cccaagggaa
     3421 gaacttttta atttaattta tcatttcaac attttatcat tcatcccgct aagcgggaca
     3481 agtttcaaca ttttat
//
LOCUS       DAOSID010000078         3448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_23898_length_3448_cov_17.100501, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000078 DAOSID010000000
VERSION     DAOSID010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23898_length_3448_cov_17.100501"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PK674_03210"
     CDS             complement(<1..250)
                     /locus_tag="PK674_03210"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HOG15572.1"
                     /translation="MEFEGIITMMGTEEEVGANNLRKITFVLEEESDREYKSSIAIDA
                     YGEKVDLVKNFKEGDKVKVGLNFRAREYNGRWYNGISAW"
     gene            complement(291..740)
                     /locus_tag="PK674_03215"
     CDS             complement(291..740)
                     /locus_tag="PK674_03215"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="signal peptidase II"
                     /protein_id="HOG15573.1"
                     /translation="MSIKNILKISFLSIILIVIDQVSKHFFYDKLFLNDLSIIQPVFN
                     AGISRSIPIPQVVIIVLTFLILGLLVYLYYKKQISKWSTIFLIGGAVSNLIDRVFLGG
                     VRDFILLFKRFPIFNLADVFINTGVVLIVLKELFGIGQKYSGKRKNY"
     gene            complement(778..1293)
                     /locus_tag="PK674_03220"
     CDS             complement(778..1293)
                     /locus_tag="PK674_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15574.1"
                     /translation="MQKFCITFAGCVGSSKTPISNYISTKLNLPIFNNDAIRSEVIEN
                     IGFLDIEKHRKIRNERLQEILESGISFIADISVDRERENFKEKLSEHGYIWFIISTDL
                     SKDLLIRLYKAKGYDESLQRLDTIFQDHEKFLEKFSDDIGLQIGDEQFLNRLALSFNA
                     VKQFIDNIVTK"
     gene            complement(1341..1922)
                     /locus_tag="PK674_03225"
     CDS             complement(1341..1922)
                     /locus_tag="PK674_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15575.1"
                     /translation="MGKYLVILGAIVSFTGVLLYIREVFRGKAKPNKVSWLLWSVAPM
                     IATVAALSQGVRRSVLPVFMSGFAPFLVFLVSLFVKQSYRKLSYFDYICGALSLLALL
                     LWGITKNPNIAIIFAILSDFAAATPTLKKSWQHPDSESPIGFIGGLFNATTAFFVLQS
                     FSFAELAFPIYLVVIDITLISFIILGRKSKKQK"
     gene            complement(1956..>3448)
                     /gene="rlmD"
                     /locus_tag="PK674_03230"
     CDS             complement(1956..>3448)
                     /gene="rlmD"
                     /locus_tag="PK674_03230"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=25
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOG15576.1"
                     /translation="ERIGYGGIGITKSDDGKKILIKGGALPGSIVDCVIKKNKKDYLE
                     GHITKVHEYDSKYIDGEIFCPHFFSNIDPNLGDNSSIEKHKIGCGGCKWQMISYPKQI
                     ELKQEIVEDCFRKVKEKPSFLPIVASPKEKGYRNKIEFSFGVYMAAREGIESKWNLGF
                     HKQGEFSKIIDVDSCGLISDKANSVFEYIKKLCFESGLSTYDQKFNRGFFRHLVIREG
                     VNTKQLMINLAVYPLDKKDKININNRENLKKTFKEDEFIKENITSFVITYNDGLADIV
                     KGPNISTENIIGDGYIYEKLILDGIGQEKVESTFRISPFSFFQTNTHGAELLFQTAMN
                     NVLEVGEIKGKILDLYCGTGSIGISFLKAGVGNSLIGIEIVEEAIQDANYNAEINGIK
                     DNCKFIASPSEKVLSKNPEIENELTNIGLVIVDPPREGLHKNVVERISQLKEDNNFKL
                     LYISCNPSTMARDIELFIEKGFKVKSLQAVDMFPHTHHIETVGVLV"
BASE COUNT         1157 a          578 c          410 g         1303 t
ORIGIN      
        1 tccatgcaga gatcccattg taccatctac cattgtactc tctagctcta aaatttaaac
       61 caacttttac tttgtctcat tctttaaaat ttttcactag atctactttt tctccataag
      121 catctatagc gatagaagat ttgtactctc tatcagattc ttcttccaaa acaaaagtaa
      181 ttttgcgtaa attgtttgct caaacttctt cttcagttca catcatagtg ataattcctt
      241 caaattccat tttctaattg gtaattaaat aaaattgtaa ttgttgtata ctaataattt
      301 tttctttttc aagagtattt ttgtcatatt ccaaaaagct cctttaaaac tatcaaaacc
      361 acccctgtat ttataaacac atctgctaaa ttgaatatgg gaaatcgttt aaatagtaaa
      421 ataaaatctc ttactcatca taaaaaaact ctatcaataa ggtttgaaac agctcatcat
      481 atcaagaata ttgtagacca ttttgaaatt tgttttttat aatatagata aactaatagt
      541 cataaaatca aaaaagttaa aactattatt accacctgag gaataggtat tgatcgtgat
      601 atccctgcat taaagacagg ttgaatgatt gatagatcat tcaaaaataa tttatcataa
      661 aagaaatgtt ttgatacttg atcaattaca atgagaataa tagacaagaa acttattttt
      721 aatatgtttt tgatagacat gatattttat ggtgttttgt aaataaatag aagattatta
      781 ttttgtaact atattatcta taaattgttt tacagcattg aatgatagtg ccaatctgtt
      841 caaaaattgt tcatctcaaa tttgaagtcc tatatcatcg gagaattttt ccaaaaattt
      901 ttcatgatct tggaaaatgg tatccaatct ttgtagtgat tcatcatatc ctttcgcttt
      961 atataatctt atcaataagt ctttacttaa atctgtacta ataataaacc aaatataacc
     1021 gtgttcagag agtttttcct taaaattctc ccgttctcta tcaacgctta tatctgcaat
     1081 aaatgaaatt caggattcta aaatttcttg aagtctttcg tttctgattt ttctatgttt
     1141 ttctatatcc aaaaatccta tattttcgat cacttcagaa cgaatagcat cattattaaa
     1201 tattggtaaa tttaacttag tgcttatgta gtttgaaatc ggtgttttag aacttcaaac
     1261 acatccagca aaggtaatac aaaatttttg catgccatgg tataataaat aaaaattatt
     1321 gtttttatag tcaaatgtaa ttatttttgc ttcttacttt ttcttcctaa tataataaat
     1381 gaaatcaggg ttatatctat aacgaccaaa taaataggaa aagccaattc agcaaagcta
     1441 aatgattgca atacaaaaaa tgctgttgta gcattaaata atcctccaat aaaccctata
     1501 ggtgattcac tatctggatg ctgccaagat tttttcaagg taggagtggc tgctgcaaag
     1561 tcacttaaaa tagcgaatat tatagctata ttgggatttt tggtgattcc ccataacaag
     1621 agagctagta aagataatgc tcaacaaata tagtcgaagt aggaaagttt tcgataagat
     1681 tgtttcacaa aaagtgaaac taaaaaaact aaaaatggag caaacccaga cataaaaaca
     1741 ggtaaaacag accgccttac tcactgagaa agggcagcta ctgtcgcaat cattggagca
     1801 actgaccaaa gtagccaaga aactttattt ggttttgctt ttcatctaaa aacttctctt
     1861 atatacaaca atactcctgt aaagctgact attgccccta aaattactaa atactttcac
     1921 atctgtgtta taaattacaa aattaaaagc cttttctaaa ctaaaactcc aacagtttcg
     1981 atatgatgag tgtgaggaaa catatctaca gcttgtaaag atttaacttt gaatcatttt
     2041 tcaataaaca attcaatatc tctggccata gtgctaggat tacacgatat gtacaataac
     2101 ttgaaattgt tatcttcttt taactgactg atccgttcaa ccacattttt gtgtaatcat
     2161 tctctaggag gatcgacaat taccaatcat atattggtca attcattttc tatttctggg
     2221 tttttgctta acactttttc agaaggggag gctataaatt tacaattgtc ttttatccca
     2281 tttatttctg cattgtaatt agcatcttga atcgcttctt ctactatttc tattccgatt
     2341 aaactatttc atacacctgc tttcaaaaaa ctgattccta tactaccagt tccgcaatat
     2401 agatctaata tttttcattt aatttctcac acttccaata cattgttcat cgccgtttga
     2461 aataataatt ctgctcaatg agtgttggtc tgaaaaaatg aaaacggaga aattctaaaa
     2521 gtggattcta ctttttcttg tcctattcaa tcaagaatta atttttcata aatatatcaa
     2581 tctcagatta tattttcagt gcttatgttt ggtcatttta caatatcagc tagtccatcg
     2641 ttataagtta tcacgaaact tgtaatattt tctttgataa attcatcttc tttaaatgtt
     2701 ttttttaagt tttcccgatt attaatattt attttatctt ttttatccaa aggatataca
     2761 gccaaattta tcattaactg ttttgtattt actcattctc taattaccaa atgacgaaaa
     2821 aatcatctat taaatttttg gtcataagta gacaatcctg attcaaaaca aagttttttt
     2881 atatattcaa aaacactatt tgctttgtct gatattaatc aacagctatc aacatctata
     2941 attttactaa attctcattg tttgtgaaat cataggttcc atttgctttc tattcattct
     3001 ctggctgcca tataaactca aaagctaaac tctatcttgt ttctatatca tttttctttt
     3061 ggagaagcaa ctattggtaa aaaacttggt ttttctttta cttttctaaa acaatcttcc
     3121 actatttctt gtttcaattc tatttgtttt ggatagctga tcatttgcca tttacatcat
     3181 ccacatcaaa ttttgtgttt ttcaattgag ctattgtcgc ctaaatttgg atcaatattg
     3241 ctaaaaaaat gcggacaaaa aatctctcca tcgatgtatt tagagtcata ttcatgaact
     3301 tttgtgatat gtcattccag atagtctttt ttattttttt tgatcacaca atctacaata
     3361 ctaccaggta atgctcctca ttttattaaa atctttttac catcatcact cttagttatc
     3421 ccaatccctc aatatccaat tctttcta
//
LOCUS       DAOSID010000079         3444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_23929_length_3444_cov_13.679256, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000079 DAOSID010000000
VERSION     DAOSID010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23929_length_3444_cov_13.679256"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="PK674_03235"
     CDS             complement(<1..934)
                     /locus_tag="PK674_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="ATP-binding protein"
                     /protein_id="HOG15577.1"
                     /translation="MTKTFTNLGLEGYEILVEADANKSLPGIEIIGLPDAAIKESKER
                     LRATFRNVGIILPNKRFVLNLSPSDIRKVGTSFDLSMAVAILVLLQEGRVHHQKDLEK
                     YLFFGELGLDGTIKRVNGLLPSVISAIKKGYKDFFVPSENLYELEYISGITIYPLDNF
                     LQLVGYFVDGKDLQCVSQSKDVESLYNVQYDFEIDFEHIKGHILAKRALSIAAAGLHN
                     VFMIGAPGSGKTMLSKALKSVLPPLGFQEILEVSQIYSVVGKLNKDHPLIVQRPFRQV
                     HHTASKISIVGGGSNLTPGEVSLAHKGVLFFDEIT"
     gene            complement(1123..1503)
                     /locus_tag="PK674_03240"
     CDS             complement(1123..1503)
                     /locus_tag="PK674_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15578.1"
                     /translation="MSLIQFPLIVMFICFMFHLFHIRRWKRRYLKRKEPILLFVKSLK
                     VLFNNDAKAYISSPPHMLGVLRFFAWLSTNNEEGQYNRIVHKLPRNRCEVWREFNRNI
                     VSFEDEQKKQYQKILFELERFYSL"
     gene            complement(1864..3051)
                     /gene="kbl"
                     /locus_tag="PK674_03245"
     CDS             complement(1864..3051)
                     /gene="kbl"
                     /locus_tag="PK674_03245"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOG15579.1"
                     /translation="MYSNIKHYLQKELDSIKEAGLYKNERIIVSPQEAEIKLENGQTV
                     LNFCANNYLGLSNHPELKKAAEEALKSHGYGMSSVRFICGTTDLHKKLEQKISEFFGT
                     EDTILYAACFDANGGVFEPLLSAEDAIISDSLNHASIIDGVRLCKAQRYRYENANMED
                     LEAKLIEAKDARFKLIVTDGVFSMDGNIAPMDKIVELANKYDAMVMVDECHSAGVVGA
                     TGRGVTEYFNIRGQVEIITGTLGKAFGGAIGGFTTGKKEIIELLRQRSRPYLFSNSIP
                     PVVVVAGIKVFDMMASTAELQDKLHQNTEYFVEKMKGLGFDIKPTASAICALMLYDAK
                     LSQDFARELLDEGIYVIGFYYPVVPKEQARIRIQLSVAHEIHHLDKAISAFEKIGKKL
                     GVI"
     gene            complement(3137..>3444)
                     /locus_tag="PK674_03250"
     CDS             complement(3137..>3444)
                     /locus_tag="PK674_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15580.1"
                     /translation="IKIKMAVKKTTKKKIDLEKKVDEVKGVAKETAKKVEVHSKNFFS
                     KIGDWWIKSTWEERIYMILGIILLIIGIYVLRTIVGGLILIAIGYLFVTGYFVNKKN"
BASE COUNT         1180 a          593 c          433 g         1238 t
ORIGIN      
        1 ctgtaatttc atcaaaaaac aaaactcatt tatgtgctaa gctcacttct catggagtta
       61 aattggatca tcctcaaact atagaaattt tggatgccgt atgatgaact tgtctaaaag
      121 gtctttggac tatcaaaggg tgatctttgt ttaattttca tactacagaa taaatctgtg
      181 atacttccaa aatttcttga aatcaaaggg gcggtaaaac actttttaga gctttactca
      241 acatagtttt cccacttcat ggagctcaaa tcataaaaac attgtgtaat ccagcagcag
      301 cgatagataa tgctcttttg gctaaaatat gtcatttgat atgttcaaaa tctatctcaa
      361 aatcatattg aacattgtac aaactttcta catcttttga ttgagaaaca cactgtaaat
      421 cttttcaatc tacgaaatat caaactaatt gtaaaaagtt atccaaagga taaatagtaa
      481 ttcacgaaat atactctaac tcatataaat tttcacttgg aacaaaaaaa tctttgtatc
      541 attttttgat agcagaaatt acggatggaa gtagtcaatt tactcttttg atagtaccgt
      601 ccaatcccaa ctctccaaaa aacaaatatt tttcaagatc tttttggtga tgaactcttc
      661 attcttgtaa aagaaccaat atagctactg ccatagaaag atcaaaggaa gtaccaactt
      721 ttctaatatc cgaaggagat aaatttaaaa caaatctttt atttggtaaa attataccca
      781 cattgcgaaa agtggctctg agtctttctt tggactcttt gatagccgca tctggaagtc
      841 aaataatctc tatccctggt aaagacttgt ttgcatctgc ttctactaaa atctcgtaac
      901 cttctagtcc aaggtttgtg aatgtttttg tcattcgtac cattttcttg ttcaaataat
      961 aattaaatat caactttgaa tttcccttag attgaaataa tgtatatttt ttataataca
     1021 aaaatcaaga gattttttga caaacaagaa atgggccatc aaaagcaaat attgtaaaaa
     1081 taaaaaacgg ggagtagtta ctcttcccgc ttttttgttt gtctagagag aataaaatct
     1141 ctctaattca aacaaaattt tttgatattg tttcttttgt tcatcctcaa aagaaacaat
     1201 atttctatta aattctctcc aaacctcaca cctattgcgt ggaagtttgt gcacaattct
     1261 attatattga ccctcttcat tatttgttga gagccaagca aagaatcgca acaccccaag
     1321 catgtgaggt ggagaagaaa tatatgcttt agcatcattg ttgaaaagaa ctttcaaact
     1381 ctttacaaag agcaatattg gttcttttct ttttaaatag cgtcttttcc accttcgtat
     1441 gtggaaaaga tgaaacatga aacaaataaa catcactatt agagggaact gtataagact
     1501 cattcccaaa tagtgaagtc tcataataat caatattatt accatagtaa ataatactga
     1561 aattatggga gcagaattct taatcgatag atctcgattt tttatcgaga caatatcatc
     1621 cacgatttgc tccaaaagcg tggatttggg tttgaagtaa cattttgtat tcataatttt
     1681 tttattttta ttattttttt acacttatga atatatattt tttccaaaag aagtcaagct
     1741 tagttttttc acaaaatcat taatctaaat ctttctttac aattgcaaaa ttttttaatc
     1801 aaaaaatagc agacacaaca attaatatcc gctatttttc tataaataag ataaatctct
     1861 atattagatc actcataatt tctttcaaat cttttcaaaa gctgatatag ctttatccaa
     1921 atgatgaatc tcatgtgcta cagaaagttg aattctaatt ctagcttgct ctttaggaac
     1981 aactggataa taaaatccta tcacataaat tcattcatcc aaaagctctc tagcaaaatc
     2041 ttgagaaagt tttgcatcat aaagcatcaa agcacatata gccgaagctg taggtttgat
     2101 atcaaaaccg agtcatttca ttttttctac aaaatactca gtattttgat gcaatttatc
     2161 ttgtaattct gctgtactag ccatcatatc aaacactttt attcctgcta ctactactac
     2221 tggtggtata gagtttgaga ataaatatgg tctagagcgc tgtctgagta attctatgat
     2281 ttcttttttt caggtagtaa atcctcaaat ggcaccacca aacgcttttc ataaagttca
     2341 agtaataatt tctacttgtc atcttatatt gaaatactca gtaactcccc tacctgtagc
     2401 tcaaacaact caagcagaat gacactcatc taccatcacc atagcatcat atttgtttgc
     2461 aagttctact attttatcca ttggagcaat atttcaatcc attgaaaaaa ctccatctgt
     2521 tactataagt ttgaatctag catcctttgc ttctataagt ttagcttcca aatcttccat
     2581 atttgcattt tcatatctgt atctttgtgc tttacataat ctaactccat ctataataga
     2641 agcatgattt aatgaatcag aaataatagc atcttcagct gaaagcaaag gctcaaaaac
     2701 tcaaccatta gcatcaaaac aagccgcata aagtatcgta tcttctgtac caaaaaattc
     2761 agatattttt tgttcaagtt ttttgtgtag gtctgttgtt ccacaaataa aacgaacaga
     2821 tgacattcca tatcagtgag atttgagagc ttcttcagct gcttttttta attctggatg
     2881 gttggataat cataaataat tgtttgcaca aaaatttaat actgtttgac cattttcaag
     2941 tttgatttct gcttcttgtg gtgaaacaat aatgcgttca tttttgtaaa gtcatgcttc
     3001 tttgatagaa tccaactctt tttgtaaata atgcttaatg tttgagtaca tttttagcca
     3061 acctaaaaaa taaaaatatt atttaacata aaaataaata attttttaca tttcattttt
     3121 tattcgtaaa attaatctaa ttttttttgt tcacaaaata tcaggtaaca aatagatatc
     3181 caatagcaat taaaattaat ccaccaacaa ttgtacgcaa aacatatatt caaataatca
     3241 aaaggatgat tcataaaatc atataaattc tttcttccca tgtagattta atccaccaat
     3301 ctccgatttt agaaaaaaag ttttttgaat gaacttcaac ttttttagca gtttcttttg
     3361 ctacaccttt tacctcatct accttttttt ctaaatcaat ctttttttta gtcgtttttt
     3421 taacagccat ttttatttta atat
//
LOCUS       DAOSID010000080         3425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_24101_length_3425_cov_13.986053, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000080 DAOSID010000000
VERSION     DAOSID010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24101_length_3425_cov_13.986053"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2015
                     /locus_tag="PK674_03255"
     CDS             <1..>2015
                     /locus_tag="PK674_03255"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOG15581.1"
                     /translation="ITPYHGDASFLVGPTKKTQELREKCKIALKEERDNNGCRSIDTE
                     TISTITSHKPGYIDQKNEVIVGLQTDEVLKRAMKPFGGIRIVERAIKERGLEMSDRVK
                     NIFHYAKNHNDAVFSAYDEEIKQFRSKHIITGLPDNYARGRIIGDYRRLALCGIDALL
                     ENKEWEKSNITGDMTDEKVRLREEISQQIQALKDIKTMADSYGFDISKPATNAKEAVQ
                     WLYFAYLAAIKEQDGAAMSLGNVSSFLDIYIEKDLKEGKITEDEAQELIDQFVMKLRM
                     VRHLRPASYDEIFGGDPTWVTESIGGQFMDGRTKVTKTSFRFLHTLYNLGASPEPNLT
                     VLWSKDLPENFKKFCAKVSIDTSSIQYENDDLMVCTRGSDDYGIACCVSYQELGKRIQ
                     HFGARANLVKGLLLAINEGKEEEGGEKVLDIKKLSNGELKYEEVWENFKIAMDEIARV
                     YAKSMYIIHYMHDKYYYERAQMAFIDADYGVDIAYGIAGISIIADSLSAIKYAKVTPI
                     RNKQGVATDYKIEGDFPKFGNDNDKVDSIAQEVVEYFNEQLKKHKAYKDANPTLSLLT
                     ITSNVMYGKNTGATPDGRKAGEAFAPGANPMHGRDTSGAIASLNSVSKFKYECSQDGI
                     SNTFTIIPKSLGVDEKEQIENLVDMLNGYFTKKAHHINVNVLNKEKL"
     assembly_gap    2016..2115
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2141..2866
                     /gene="pflA"
                     /locus_tag="PK674_03260"
     CDS             2141..2866
                     /gene="pflA"
                     /locus_tag="PK674_03260"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HOG15582.1"
                     /translation="MLKVHSIESFGTQEGPGIRFVLFLQGCFFKCIYCHNPDTIPING
                     GKEMEIDKIVEMIKQTVPYFGTRGGCTISGGEPLVQAKNLIDLFKKLKKENIHTCLDT
                     NGCLWNKDVEELLEYTDMILLDIKHIDNDWHKKITGQENKNVFNFLDYLEKNNKRTWI
                     RYVLVPGYSDQEEYINMLGEKYRKYKCIERLEILPYHTFGEHKWKELGRKYELHGVAA
                     PTEEAVQKAENLFKKYFKEVYVR"
     gene            2890..>3425
                     /locus_tag="PK674_03265"
     CDS             2890..>3425
                     /locus_tag="PK674_03265"
                     /inference="COORDINATES: protein motif:HMM:NF017900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOG15583.1"
                     /translation="MKITVGISNRHVHLSRKDADLLFGEGYELTKIKDLSQPGQFATE
                     ECVVLKGPKGEISKVRILGPYRKDTQIEVLLGDNYKLGINAPVRLSGDLENTPGLEII
                     GPNGSITTKKGAIVAQRHIHMNLEEAKEFGLENGQIVSVKTEGERGLIFENVIIRADS
                     KGALDFHIDMEEANAAGV"
BASE COUNT         1366 a          456 c          581 g          922 t
ORIGIN      
        1 attactccat atcatggaga tgcctctttt ttagtatgac caacaaaaaa aactcaagaa
       61 ttacgagaaa aatgtaaaat tgcactaaaa gaggaaagag acaacaattg atgcagaagt
      121 atcgatactg aaactatatc aacgatcact agccataaac catgatacat agaccaaaaa
      181 aacgaagtga tagtttgact tcaaacagat gaagttttga aaagggcgat gaaaccattt
      241 gggggtatca gaatcgtaga aagagcgatc aaagaaagat gattggaaat gtccgacaga
      301 gtaaaaaata tttttcatta tgcaaaaaac cacaacgatg cagtatttag tgcttatgat
      361 gaagaaataa agcaatttag aagcaaacat attatcacct gattgcctga caactacgca
      421 agatgaagaa ttatttgaga ttatagaaga ttagctctgt gtggtattga tgctttgtta
      481 gaaaataaag aatgggaaaa atccaacatt acatgagata tgactgacga aaaagtaaga
      541 cttagagaag aaatatcaca acaaatccaa gctttgaaag atataaaaac aatggctgat
      601 agctacggtt ttgatatatc aaaaccagca acaaatgcaa aggaagcggt acaatggcta
      661 tattttgcat atctagcagc tatcaaagaa caagatggag ccgctatgtc acttggaaat
      721 gtatcaagtt tcttagatat ttatatagaa aaagacttga aagaatgaaa aatcacagaa
      781 gatgaagcac aagaacttat agaccaattt gtaatgaaac ttagaatggt tagacatctt
      841 agaccagcat cttatgacga aatttttggt ggagatccta cttgggtaac agaatctata
      901 tgatgacaat ttatggactg aagaacaaaa gttaccaaaa cttcattcag attcttgcac
      961 acattgtata atttatgagc atctcccgaa ccaaatctaa cagtactttg gtcaaaagat
     1021 ttgccagaaa atttcaaaaa attctgtgca aaagtatcaa tagatacctc ttctatacaa
     1081 tatgaaaacg atgatttgat ggtttgtact cgtggttctg atgattatgg tattgcttgc
     1141 tgtgtatcat atcaagaact atgaaaaaga attcaacatt ttggtgctag agcaaattta
     1201 gtaaaaggat tacttttagc catcaatgaa tgaaaagagg aagagtgatg agaaaaagtt
     1261 ttggacataa aaaaacttag caactgagaa ctaaaatacg aagaagtttg ggaaaacttc
     1321 aaaatagcaa tggatgaaat tgctagagtt tatgcaaagt ctatgtacat catacactat
     1381 atgcatgata aatactatta cgaaagagcc caaatggcat ttatagatgc tgattatgga
     1441 gtggatattg cttatgggat tgcaggaata tctattatag cagattcgct ttccgctata
     1501 aaatatgcaa aagtaactcc aatcagaaat aaacaatgag tagctacaga ttacaaaata
     1561 gaatgagatt tcccaaaatt tggaaatgat aatgataaag tagattcaat agcacaagaa
     1621 gtagttgaat atttcaacga acaactcaaa aaacataaag catacaaaga tgcaaatcct
     1681 actctttcat tactaacaat tacatcgaat gtaatgtacg gaaaaaatac atgagcaact
     1741 ccagattgaa gaaaagcttg agaagcattt gctccaggag caaacccaat gcatggtcgt
     1801 gatactagct gagctattgc atcattaaat tctgtgagta aattcaagta tgaatgctct
     1861 caagactgaa tctcaaacac atttactatt ataccaaaat cattaggtgt agatgaaaaa
     1921 gaacaaatag aaaatttagt agatatgcta aattgatatt tcacaaaaaa agctcatcac
     1981 atcaatgtaa atgttttgaa taaagaaaaa cttatnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnntttat aaactttatt aacctaatcc atgcttaaag tacactctat
     2161 agaaagtttt tgaactcaag aatgaccttg aattagattt gttttatttc tacaatgatg
     2221 ttttttcaaa tgtatatatt gccacaatcc tgacactatt ccaataaatt gatgaaaaga
     2281 aatggaaata gacaaaatag tagagatgat aaaacaaaca gttccttatt tttgaactag
     2341 aggtggttgt acgatttcag gtggagaacc actagtacaa gcaaaaaacc taattgacct
     2401 attcaaaaaa ttaaaaaaag aaaacataca tacttgtcta gatacaaact gatgtctttg
     2461 gaataaagat gtggaggaat tgttggaata taccgacatg attttactgg acattaagca
     2521 tatagacaac gattggcaca aaaaaattac atgacaagaa aataaaaatg ttttcaattt
     2581 tttggattat ttggaaaaga acaacaaaag aacatggatt agatatgttt tagtgccttg
     2641 atattctgat caagaagaat atataaatat gctatgagaa aaatatagga agtacaaatg
     2701 tatagaaagg ctagaaattc tcccttatca tacttttgga gagcataaat ggaaagagtt
     2761 atgacgaaaa tatgaattac actgagtagc agctccaaca gaagaagcag tgcaaaaagc
     2821 tgaaaatttg ttcaaaaaat acttcaaaga agtctatgtt agatagtttt atattttttt
     2881 aacaaaaaaa tgaaaataac agtttgaatt tcaaatagac atgtccatct ttcaagaaaa
     2941 gatgctgatt tgttgttttg agaatgatat gaattaacaa aaatcaaaga tttatcacaa
     3001 ccatgacaat ttgccactga agaatgtgtg gtcttgaaat gacctaaatg agagatatca
     3061 aaagttagaa ttttatgacc atacagaaag gatactcaaa tagaagtact actctgagac
     3121 aattataaat tgggaataaa cgccccagtt agactttctg gagatttgga aaacacacca
     3181 tgattggaaa ttatatgacc aaatggaagt attacaacaa aaaaatgagc gattgtagcc
     3241 caaagacata tccatatgaa tttagaagaa gctaaagaat tttgactcga aaacggtcaa
     3301 atcgtaagcg ttaaaacaga atgagaaaga tgattaattt ttgaaaatgt aataattaga
     3361 gcagattcaa aatgagcttt ggattttcat atagatatgg aagaagctaa tgcagctgga
     3421 gtaaa
//
LOCUS       DAOSID010000081         3391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_24399_length_3391_cov_14.643285, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000081 DAOSID010000000
VERSION     DAOSID010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24399_length_3391_cov_14.643285"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1543
                     /locus_tag="PK674_03270"
     CDS             134..1543
                     /locus_tag="PK674_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="pyruvate kinase"
                     /protein_id="HOG15584.1"
                     /translation="MANFNFSKIIAGVGPVLAKETVLSKVINMVDVFRLSLSGGFDDN
                     NKKYIETIMKLDNSKTIMMETKGCDIRVKNLVDIKLKKGATINIDYSEYAQEGDKQVF
                     IDYQKLGELGVGAKIRFEQSDILVEVSEIVADDNINCKVLEGGTLLQFDRVRFLNNPI
                     DFGVLTERDKKDILRGLEYGIHMIALSGVKSPDDILELKSFLSEQNKGNMKIVAKIET
                     LEGLKNLDAINAISDSIIFVFDKITEEMKALKLTKEGLIKKIQSTGKPVAVSFISSVG
                     NKDYPFRTKEGIKEFCDLSIDSFVLEHIIEEEETLPIITETYELLEKCESEVKDVEAK
                     RFDNDEDFAVRDYIIYSAYRITRELDIKAIVCFTDNGYSSARLSSLAPKVPVITFTKS
                     DETYRFLNMIRGVRGYKISESFNYENLKRIGKEMIRIIFKGNISLDDKIVIVQSNEYQ
                     KDERSDMINGVELYKFKNI"
     gene            1779..>3391
                     /locus_tag="PK674_03275"
     CDS             1779..>3391
                     /locus_tag="PK674_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15585.1"
                     /translation="MGNTRQKLNNMNSINKVDSNLQEGLQNLFELQKGLKSQDFQNLL
                     ETFGDLNTDSINLISKILSENKIKVLDLTSLSIDVNNILEYDERDELNDNLQNIIGHI
                     FIKKLYDIVAPDNELLNLFILTFGEFEKIKNKKTSFKSIKINIKDFLEKYPNLLDEIK
                     ISNQEVFDGLKRNRYNVNERNSFGDQDLSNQHIIATLLSWQNKETIRKLDGHQNNVYS
                     QIQEVGKPVDGEFPLCESNDEILRLKEEIYDIEEDGTTDRQNEINKINKSIRKEYLKK
                     MGGSKENNDVVEVLEKLVENNFNFGELDGKEQTIFANKFIEKTLGSNNEFLSSLNVDN
                     EAYKDFLRELFDFEKLEHELKITLADGNEIILDIKKGFHSGEHNQFLDVENFKETKNF
                     PIFFDINLEKNPPEIIELLEHKENGLLSCAQNHDGVFGSYFTKNGEICLGNNYKIKLE
                     GVDLTVDELDSMFNKDKDDLEILLKKFGLWEKTKTHFYEITKNKELLKSFADNPHSGS
                     IFEEMLKKLDLEIESKNLKFDLNNINKIS"
BASE COUNT         1385 a          331 c          557 g         1118 t
ORIGIN      
        1 aaatatcaag caaataagta taaaaaacga acttaactct gcaatagcac aaatacttta
       61 caaactttat aaactttatg aactttataa actttataaa ctttacgaac atttaacttt
      121 taactaactt agtatggcta atttcaattt ttcaaaaatc atcgcatgag tatgacccgt
      181 tttagcaaag gaaacagttc tatcaaaagt tatcaacatg gtagatgttt ttagacttag
      241 tttgtctggt gggtttgacg ataataataa aaaatatatt gaaacaatta tgaagttgga
      301 caatagtaaa actattatga tggaaactaa gtgatgcgac ataagagtaa aaaatttagt
      361 ggatataaaa ctaaaaaaat gagctactat aaatatagac tattcagaat atgcacaaga
      421 gtgagataaa caagttttta tagattatca gaaattatga gaattgtgag ttggtgcaaa
      481 aattagattt gaacaaagcg atattttagt agaagttagt gaaattgtag ctgacgataa
      541 cataaattgt aaagtgttgg aatgatgaac acttttacaa tttgatagag ttagattttt
      601 gaataatcca atagattttg gtgttttgac agaaagagat aaaaaagata ttttacgatg
      661 attggaatat gggatacata tgattgcttt atctggtgtc aaaagcccag atgatatctt
      721 agaattaaaa tcatttttaa gtgaacaaaa taaatgaaat atgaaaattg tagcaaaaat
      781 agaaaccttg gaatgattga aaaatttaga tgcaattaat gctatttcag acagtattat
      841 ctttgttttt gacaagataa cagaagaaat gaaagcatta aaattaacaa aggagggctt
      901 gataaaaaaa atacaaagta ctggtaagcc tgttgctgtt agttttattt ctagcgtatg
      961 aaacaaggac tacccgttta gaacaaagga gtgaatcaaa gaattctgtg atttatccat
     1021 agattctttc gttttagaac atattataga agaggaagaa actctaccaa ttattactga
     1081 aacttatgag ttattggaaa aatgtgaatc agaagtaaaa gatgtagaag ctaaaagatt
     1141 tgataatgat gaggattttg ctgttagaga ttatatcatc tatagtgctt acagaataac
     1201 tagagagttg gatataaaag ctatagtttg ttttactgat aacggatatt cttctgcgag
     1261 actttcatct ttggctccaa aggtgcctgt aattactttt actaagtctg atgaaaccta
     1321 tagatttttg aatatgatac gaggagttag gtgatataaa atttcagaat cttttaatta
     1381 cgaaaatcta aaaagaatag gtaaagaaat gattagaata attttcaagt gaaatatttc
     1441 tttggatgat aagattgtga ttgttcagtc aaatgaatac caaaaagatg aaagaagcga
     1501 tatgattaac ggagtagaat tatataaatt caaaaatatc taattttttg tccttatttt
     1561 tttgtatttg tgttctaatc tatttataat atcattagtt gtaaacaaga gttttaatat
     1621 ctaatgaagt ttttttaata ataagattta gcgaggaaat tttcgatttg acatttgttt
     1681 gttattgttt ataatatgct tgtatcgttg taataatttg caaattttat attttggtat
     1741 atacttattt tagtatttta tgtactgtaa ataagacaat gggaaataca agacaaaaac
     1801 taaacaatat gaattcaata aacaaggtag attccaatct tcaagaagga ttgcaaaact
     1861 tgttcgaatt gcaaaagtga ttaaaatcgc aagattttca aaacctttta gaaacttttg
     1921 gtgatttaaa tacagactct ataaacttga tttctaaaat tctttcagaa aacaagatca
     1981 aagttttaga tttaacgagt ctaagcatag atgtaaataa tatattagaa tatgatgaaa
     2041 gggatgagtt aaatgataat ctacaaaata ttatcggaca catattcata aaaaagttat
     2101 acgatatagt tgctcctgat aatgaacttt tgaatctttt catactaact tttggtgaat
     2161 ttgagaagat aaaaaataaa aaaacatcat ttaagtctat aaaaattaat ataaaagatt
     2221 ttttagaaaa atatccaaat ttgttagatg aaataaaaat ttcaaatcaa gaagtttttg
     2281 atggtttaaa gaggaataga tacaatgtaa atgaaagaaa tagtttttga gatcaagacc
     2341 tttctaatca gcacattatt gctactctat tatcttggca aaacaaagaa actattcgta
     2401 agttggatgg acatcaaaac aatgtttact ctcaaataca agaagtagga aaaccagttg
     2461 acggtgaatt tccattgtgt gaatctaatg acgaaatatt gagattaaag gaggagatat
     2521 atgatattga ggaagattga acaacagata ggcaaaatga aattaataaa attaataaat
     2581 caatccgaaa agaatactta aagaagatgg gagggagtaa ggaaaataat gatgtggtag
     2641 aagttttgga gaaattagta gaaaataatt ttaacttttg agagctagac tgaaaagaac
     2701 aaactatatt tgcaaataag tttattgaaa aaacactttg aagtaacaat gagtttttat
     2761 cgagtttgaa tgttgataat gaagcttaca aagatttttt aagagaactt tttgactttg
     2821 aaaaactaga gcatgaatta aaaattactc ttgcagatgg taacgaaata atcttagata
     2881 ttaaaaaatg atttcattca tgagaacata atcaattttt ggatgtagaa aactttaaag
     2941 aaacaaaaaa ctttccgata ttttttgata taaatttaga aaaaaatcct ccagagataa
     3001 tagaattgtt ggaacataaa gaaaatggac ttttgagttg tgctcaaaat catgatggag
     3061 tatttggctc ttatttcaca aaaaattgag agatttgttt aggtaacaac tataaaataa
     3121 aattggaatg agtagatcta actgtagatg aacttgattc tatgtttaat aaagataaag
     3181 atgatttaga aattttattg aaaaagtttt gactttggga aaaaactaaa acacattttt
     3241 atgaaataac aaaaaataaa gaactattaa aaagctttgc tgacaatcct catagtggtt
     3301 ccatttttga agaaatgtta aaaaaattag atttggagat agagtcaaaa aatctaaaat
     3361 ttgatttgaa taatattaat aaaataagtc a
//
LOCUS       DAOSID010000082         3193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_26384_length_3193_cov_16.958572, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000082 DAOSID010000000
VERSION     DAOSID010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26384_length_3193_cov_16.958572"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..>1620
                     /gene="gpmI"
                     /locus_tag="PK674_03280"
     CDS             128..>1620
                     /gene="gpmI"
                     /locus_tag="PK674_03280"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOG15586.1"
                     /translation="MKKVMLIIRDGRGYRKETENNAIAQVDTPFTDNLMKNYPNILLN
                     TSGEDVGLPAGYQGNSEVGHMTIGSGRIIFQSLERINHSIKDGSFFEIDALKGAIENC
                     KKHSSTLHLMGILQGEGVHAHIDHLFALLDLCDKESFHNVNIHVFTDGRDAPVTKSLE
                     YLGSLEKKLKTLGFGKICTIMGRFYAMDRDKRWDRTQIAYDAICNAKTDKVFDNMIEG
                     VKESHNNGETDEFIKPIVLKGYEGIKEHDSIIFWNFRTDRTRQLTKAIVEPVFEGWNR
                     SPLNVYYVGMTQFYDSMNALVAFPELEIKNGLGEVVSNAGLRQLRISETEKYAHVTFF
                     FNGQVEAPFRNEDRILIPSPKVQTYDLKPEMSAYEITDAICEKIENDNYNLIITNLVN
                     GDMIGHTGVIDAIHKAVKTVDDCVGKLVEYGLKNGYEILVFADHGNVEDQSEERKTSH
                     TTNPVPFILVSDKKYKLESSGGLVDIAPTVLELLGIEKPNEMTGKSL"
     assembly_gap    1621..1720
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1757..3004
                     /gene="eno"
                     /locus_tag="PK674_03285"
     CDS             1757..3004
                     /gene="eno"
                     /locus_tag="PK674_03285"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOG15587.1"
                     /translation="MVKISKIHGREVLDSRGNPTVEVELTLENGVMGRAIVPSGASTG
                     VHEALEMRDGDKSRYLGKGVLNAVNNVNTTLKSNIENKEFSDFVELDNFMIELDGTKN
                     KSKLGANAILGVSMAFVNACAKNEGKWIYEFLDNGNTLPYPMMNILNGGSHADNNVDI
                     QEFMIVPVSAPNFKEGLRRGTEIFHNLKAILKAKGLATSVGDEGGFAPNLGSNEEAIQ
                     VIMEAIEKAGYSGKIKLALDVASSEFFKDGKYELSGEGKTLSASELVNLYQDRCAKYP
                     IMSIEDGMAEDDFEGWKELNTKLGDKIMTVGDDLLVTNIERLKMGIENKLSNSILIKL
                     NQIGTVSETISAVKMAHDNGMRAVISHRSGETEDTFIADLAVALNTGFIKTGSASRTD
                     RIAKYNQLLRIEERLGGNAKYGR"
BASE COUNT         1241 a          389 c          551 g          912 t
ORIGIN      
        1 tttttatttg aattttacaa atatattcat agagtttaca gagcaaaaaa acttacttta
       61 ttcacaacga aatatacaat ttccaaaaca ataaagtaat aaaattttat cctaaaaaaa
      121 taaaactatg aaaaaagtca tgttaataat tcgcgattga cgatgatatc gcaaagaaac
      181 agaaaacaat gcaatcgcac aagtagacac gccattcaca gataatttga tgaaaaatta
      241 tccaaatatt ttactcaaca caagcggaga agatgtttga cttccagctt gataccaatg
      301 aaattctgaa gtctgacaca tgacaatcgg tagtggtaga ataatatttc aatcattaga
      361 aagaataaac cacagtatca aagattgatc attttttgaa atagatgctc taaaatgagc
      421 aattgaaaac tgcaaaaaac actcctctac tctacatttg atgtgaattt tgcaatgaga
      481 atgagtacat gctcacatag atcacttatt tgctcttttg gatttgtgtg ataaagaaag
      541 ctttcataat gtaaatatcc atgtatttac tgattgaaga gatgctcctg tgacaaaaag
      601 tttggaatat ttatgaagtt tagaaaaaaa actaaaaaca ttatgatttg gtaaaatatg
      661 cacaatcatg tgaagatttt atgctatgga tagagacaaa agatgggaca gaacccaaat
      721 cgcttacgac gccatttgta atgcaaaaac tgataaagtg tttgataata tgattgaatg
      781 agtaaaagaa tctcataata actgagaaac agatgaattt ataaaaccta tagtactcaa
      841 agggtacgaa tgaatcaaag agcatgattc aataatattt tggaatttta gaacagatag
      901 aacaagacaa ttaacaaaag ccatcgttga gccagttttt gagggttgga atagaagtcc
      961 tttgaatgtt tactatgtgt gaatgactca attttacgat agtatgaatg cactagtagc
     1021 tttccctgag ctagaaatca aaaactgact tggagaagtg gtaagtaatg cttgattgag
     1081 acaattaaga atttcagaaa ctgaaaaata tgctcatgtg acattctttt ttaactgaca
     1141 agtagaagca ccatttagga atgaagacag aattttaatc ccttctccaa aagttcaaac
     1201 ttatgacttg aaaccagaaa tgtcagctta tgaaattact gatgcaatat gcgaaaaaat
     1261 agaaaacgac aattataatt tgataataac aaatctagta aactgagata tgatttgaca
     1321 tactggagta attgatgcta ttcataaagc tgtaaaaact gtagacgatt gtgtttgaaa
     1381 attagttgaa tattgactaa aaaactgata cgaaatattg gtttttgcag atcatggtaa
     1441 tgtagaagac caaagtgaag aacgaaaaac ttctcatacc acaaatccag ttccatttat
     1501 acttgtaagt gataaaaaat ataaattgga atctagtggt ggtttggtag atatcgcacc
     1561 gactgttttg gaattattgt gaattgaaaa accaaacgaa atgacaggta aaagtttaat
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaaagcaaat tttaaattat
     1741 taaataaata ttcaaaatgg taaaaattag taaaattcac ggaagagagg tattggatag
     1801 tagagggaat ccaactgtag aagtagaatt aactttagaa aattgagtaa tgtgaagagc
     1861 tatagttcca tctggagcta gtacttgagt tcacgaagct ctagaaatga gagatggcga
     1921 caaaagtaga tatttaggaa aatgagtttt gaatgctgta aataatgtaa acactacatt
     1981 aaaatcaaac atagaaaaca aagaattttc tgactttgtg gaattggaca attttatgat
     2041 agagcttgat ggaacaaaaa ataagtcaaa actatgagca aatgctattt taggagtaag
     2101 tatggctttt gtaaatgctt gtgcaaaaaa cgaatgaaaa tggatttatg agttcttgga
     2161 taatggaaat acattacctt atcctatgat gaatattttg aactgaggaa gtcacgcaga
     2221 caataatgta gacattcaag aatttatgat tgttcctgtt tctgctccaa acttcaaaga
     2281 atgattgaga cgatgaactg aaattttcca caatttgaaa gcgattttga aagcaaaagg
     2341 tctagcaact tctgtaggag atgaatgagg tttcgcccca aatctatgaa gtaatgaaga
     2401 agcgattcaa gtgattatgg aagctataga aaaagctggt tactctggta aaataaaact
     2461 agctcttgat gttgcatcta gtgaattttt caaagatggg aaatatgaat tatctggaga
     2521 aggaaaaact ttatcagcaa gtgaattagt aaacttatac caagaccgat gtgcaaaata
     2581 tccaataatg tctatagaag atggtatggc tgaagatgat tttgaaggat ggaaagaatt
     2641 gaatacaaaa cttggagaca aaataatgac agtatgagat gatcttttgg tgacaaatat
     2701 tgaaagactt aaaatgggaa tagaaaacaa attatccaac agtattttga taaaattaaa
     2761 ccaaatatga actgttagcg aaactatttc tgctgtaaaa atggctcacg acaatggtat
     2821 gagagcggta atttctcaca gatctggaga aactgaagat actttcatcg ctgatttggc
     2881 tgttgcatta aatactggat ttatcaaaac tggttcagct agtagaactg atagaatagc
     2941 aaaatataac caattattga gaatcgaaga aagactagga tgaaatgcta agtatggaag
     3001 ataaaaaaat ccctaaaata aacaaaagtc tgctatcata tagtagactt ttttgttttc
     3061 acttttggat attgtggaat aataatacaa aacatataaa tcaaatcttg aaaaacaaaa
     3121 gtattctagt atgctaaaaa tatttatatt caaaaaataa ttttggattt attggcaata
     3181 tcatatcaaa ata
//
LOCUS       DAOSID010000083         3167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_26661_length_3167_cov_12.371787, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000083 DAOSID010000000
VERSION     DAOSID010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26661_length_3167_cov_12.371787"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="PK674_03290"
     CDS             complement(<1..145)
                     /locus_tag="PK674_03290"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOG15588.1"
                     /translation="MNIQQSDPEIFQAIQDEQKRQLEGMELIASENYQSEAVLQAQSS
                     VFAN"
     gene            297..557
                     /locus_tag="PK674_03295"
     CDS             297..557
                     /locus_tag="PK674_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15589.1"
                     /translation="MAGRNSNRFGEQTFKNLVCLADNRIQHESVRRSLSLKQTVELIE
                     VLLDEEISPNASISELSQYAQRALDTLEYCKKDIAKVRGQYK"
     gene            771..1352
                     /locus_tag="PK674_03300"
     CDS             771..1352
                     /locus_tag="PK674_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15590.1"
                     /translation="MRYVDGIPKPVIGCDIDDVVFHLLPNLIKYYNLKYGTNKALKDV
                     WDLRYESVNICDIVEKHKLYKNMMPTRILHFLKQKRKDIYLIMITARKDIYVDDTMSE
                     LYNHGLEFDDIFMGEKKSKVCRLENVDHFFDDALHNVIDVYENAPKTKPYLVKRAWND
                     REEVNKLEAKGIKVPNGIIKLTEDEVYEVLKSI"
     gene            complement(1373..3064)
                     /locus_tag="PK674_03305"
     CDS             complement(1373..3064)
                     /locus_tag="PK674_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOG15591.1"
                     /translation="MNKFFRKIIFKFRIHRRNIVINSFLKKKKKIRISEISKRKDYSD
                     PFYMTLLDDLLDGIGKKYLRKYIYPKILEEEKNTIKNQKTILSDLLKNIKNTKIGKKY
                     GIPSILNESNIYQKFTEKLPIFDYDTYKTYIEEAKEKENIVRPDKILQFSASSGTTSN
                     KKHIPVSSESLLSASKAGICMFAEYSFKNPNTKIFKGEFFPLTGTIQYHDNNITIADV
                     SALLLLDRGKFAKNKYALDLFQLLHSEREIKREIALKYINPNKPITMMGITSRAYEIL
                     NYIQQRDAKMFDTMIKNMEVIFGGGVDVAPYMQYFKKLNLNYMGVYNASEGYFGYQDI
                     INYDNSNGDAPYKLLTNHGIFYEFLEFNSNNFDENGNCKKASEVKAIRDITKKDINKK
                     FALIITTNSGLLRYMIGDVIQFVDENLRYKITGRTRQSLNLKGEELMETHIGAVIQEL
                     SNQDKINIIYYTIGPDQEENPTKHQRVVETDTEINVSEQELAQKIDKILQKINADYKT
                     KRNNDILLKLPQITLVPQGTFYQRLKKSNRLGAQIKVPKLSSKRNYIKEVLRIVK"
BASE COUNT         1028 a          387 c          431 g         1321 t
ORIGIN      
        1 tatttgcaaa aacacttgat tgtgcttgta gtactgcttc tgattgatag ttttcgctag
       61 caattaattc cataccctct aattgccttt tttgttcatc ttgaattgct tgaaatattt
      121 ctggatcact ttgttgaata ttcataatat tgttttatat tgaataaaaa aaattttctt
      181 tagtttttta taatattttt tgttctttat tcaataaaaa ataacttttc tatattttgt
      241 gttgctttat tgtaaataat atgtataata tttttttatt ttaaatttta gaaaccatgg
      301 cttgaagaaa ttctaataga tttggagaac aaacatttaa aaatttggtt tgtttggcag
      361 acaataggat ccaacatgaa tcagtaaggc gttctttatc tttaaaacaa acagtagaac
      421 taatagaggt tcttttagat gaggaaataa gcccaaatgc ttctatttca gagttatcac
      481 aatatgcaca aagagcatta gatacattgg agtactgtaa aaaagatatt gcaaaagttc
      541 gtggccaata caaataattt atttttgata aggttaatat ttatattttg atggattttt
      601 ttttgtattc aaaaattttt gcgttttaat ttcggtgttt agcatatctt ttttgatctg
      661 taattactac aatagtaata acatttttaa atatatcttg acatttttct tgacattttg
      721 acattaatat ttattattat gtaggattta ttaataattc aaaatcaaat atgagatatg
      781 tggatggtat accaaaacct gtaatttgat gtgatataga tgatgtggtt tttcacctat
      841 tacccaattt aatcaagtat tacaatttga agtattgaac aaataaagca ctaaaagatg
      901 tttgggattt gagatacgaa tctgttaata tatgtgatat agttgaaaaa cataaattat
      961 ataaaaatat gatgccaacg cgtattttac attttttgaa acaaaaaaga aaagatattt
     1021 atctgatcat gattacagcc agaaaagata tttatgtgga tgacacaatg tcagaactat
     1081 ataatcattg attggagttt gacgatatat ttatgtgaga aaaaaaatct aaagtttgta
     1141 ggttggaaaa tgtagaccat ttttttgatg atgctttgca taatgtgatt gatgtatatg
     1201 aaaatgctcc caaaacaaaa ccttatttgg tcaaaagagc ttggaatgat cgagaggaag
     1261 tgaacaaatt ggaagcaaaa tgaattaaag ttcccaattg aataataaaa ttaactgagg
     1321 atgaagttta tgaagttttg aaaagcattt aaataattct ttgtagattc tcttatttaa
     1381 caattctcaa tacttctttt atataatttc tttttgatga aagttttggt acttttattt
     1441 gtgctcagag tctattagat tttttcaatc gttgataaaa agttcactgt gggaccaatg
     1501 taatttgagg taattttagt aaaatatcat tgttgcgctt tgttttgtaa tcagcattta
     1561 ttttttgtaa aattttatct attttttgtg ctaattcttg ttcagaaaca tttatctctg
     1621 tatctgtttc tacaactcgt tgatgttttg ttggattttc ttcttgatca ggtcatattg
     1681 tataataaat tatgttaatc ttatcttgat ttgaaagctc ttgtattact gctcctatat
     1741 gagtttccat caactcttct cattttaaat tcaaggattg tctggtccta ccagttattt
     1801 tgtatcttaa attctcatct acaaactgta ttacatctca tatcatataa cgcaacaaac
     1861 cgctatttgt ggtaataatt agtgcgaatt ttttgtttat atcttttttt gttatatctc
     1921 gtattgcttt gacttcactt gcttttttac aatttcaatt ttcatcaaaa ttattactat
     1981 taaattcaag aaattcgtaa aatattcaat gatttgttaa taatttgtat ggagcatctc
     2041 aatttgaatt atcataattg ataatatctt gatatcaaaa atatcattcg gaagcattgt
     2101 aaactcacat ataatttaaa ttcaattttt tgaaatattg catatatggt gcgacatcta
     2161 ctcctccacc aaaaataact tccatatttt ttatcatggt atcaaacatt ttggcatctc
     2221 tttgttgtat gtaatttagt atttcataag ctcggctggt tattcacatc attgtaattg
     2281 gtttgtttgg gtttatatat tttagtgcga tttctctttt gatttctcgt tctgaatgta
     2341 ataattgaaa aagatctaaa gcatatttat ttttagcaaa ctttcatcta tccaaaagca
     2401 ataaagctga cacatctgct attgttatgt tattgtcatg gtattgtatt gttcaagtta
     2461 aaggaaaaaa ctctcattta aaaatttttg tattagggtt tttgaaagaa tactcggcaa
     2521 acatacatat cccagcttta cttgcactta acaatgattc actgctaacg ggaatatgtt
     2581 ttttattgct agtagtacca gatgatgctg aaaattgcaa gattttatct ggtcgtacaa
     2641 tgttttcttt ttctttggct tcttctatat aagttttgta agtatcgtaa tcaaatatag
     2701 gtaatttttc tgtgaatttt tggtaaatat tgctttcatt taaaatgctt ggaattcagt
     2761 acttttttca tatttttgta ttttttatat ttttcaacaa atcactcaaa atggtttttt
     2821 gattttttat tgtatttttt tcttcttcta aaatttttgg atatatatat tttctcaaat
     2881 attttttacc tataccatct aataaatcat caagtaatgt catataaaat ggatcagaat
     2941 aatcttttct ttttgagatt tcactgattc taattttctt ttttttcttt aaaaatgagt
     3001 taattacaat atttcttctg tgtattctaa atttaaatat gatttttcta aaaaatttat
     3061 tcatatcaaa aataggcgat gtaaattgtt atatcaattc aattactgaa cttcaatctt
     3121 tcaaaagatt gaacatggtc aaggtgcaca tcaatctgta aatacta
//
LOCUS       DAOSID010000084         3093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_27452_length_3093_cov_12.592166, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000084 DAOSID010000000
VERSION     DAOSID010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27452_length_3093_cov_12.592166"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PK674_03310"
     CDS             <1..747
                     /locus_tag="PK674_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15592.1"
                     /translation="QIISDAIYQYEECGKDSENEKTFISYALLGTGWNSQGHTEYYLV
                     VNGQGYFLDQRGNIASSCGFGGIPTTISISQDENGPVLQEYKIARDGSEYDSSTREMF
                     SKQAYNIRRNGKYTFNNTEKTFLEQAEEYFGTTVIPESENNFDCPFCDKLRYYNWNPE
                     DDEKLQDSNDLIFNYVSNDNGKNTIYFGSDGSFEAKGSWDEGTGTRVFGKDENTVIVS
                     SNKANHIYDRYIITNQDENNLNTILEVIQR"
     gene            862..2007
                     /gene="dnaN"
                     /locus_tag="PK674_03315"
     CDS             862..2007
                     /gene="dnaN"
                     /locus_tag="PK674_03315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOG15593.1"
                     /translation="MKITINTAKLNDIVDISSRFVAKNATLPILQNIYLKASIDNLIV
                     RATDMEKYIEIETPCDIKLEGAITINAKMFSDIIKTIEDEQIEISTDPQTNTMIIKTR
                     KDNFEINGISANEYVALPDVPQENTITLDTQSLSDGIEKVEYSVTEKNFSPVLTGVLM
                     KSKEENGSKKLVFVGTDSFRLTEYKTNSSIQGDDFSLIIPKMSIIDIKKISDFAKEKE
                     SENVIVKYSNNLVAFEFEINDMKILATSLLIQGNFPDYEKEEIMPTNFNTKLIINKDD
                     CEKAIKKIGILTRDINNYIELAIDNGEAIVSSGKTDKGMGKTNIPILMEGESVSFGVN
                     GRYITDFIRTMESDEIVFNIVDNQRPIILQNKNNTNYNCVIRPLLNG"
     gene            2193..2765
                     /locus_tag="PK674_03320"
     CDS             2193..2765
                     /locus_tag="PK674_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="type II secretion system protein"
                     /protein_id="HOG15594.1"
                     /translation="MKKAFTLIEIIVIVGILAILLGTSSFLRNRNEDEKIKYGKECSN
                     YIFQEVLNQDNNIKKNKTINSGDEILLITGIQIKKENKDSYLVSMNTFNEKSELNSQN
                     TLINDKGSCTADHISNKNNYIINTDDDFMIRIQKNNNFAYKTTISVCDFGGNNCIEIS
                     KIEYNQASNKFDLKSCSLINNEGLCEEWKK"
     gene            2753..>3093
                     /locus_tag="PK674_03325"
     CDS             2753..>3093
                     /locus_tag="PK674_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15595.1"
                     /translation="MEKIEKELIFYKIMDYPIPKDKELFYFEKKIEQKKNPSSGFSRD
                     EKNKYMDTYIKRINKFGFLYKSLPFVKEIFLCNSISFNSLKRDSDIDLFIVTKSNSIR
                     RARFFSALFFK"
BASE COUNT         1342 a          407 c          435 g          909 t
ORIGIN      
        1 cagataatct ctgatgcaat ttatcagtat gaagaatgtg gtaaggatag tgaaaatgaa
       61 aagaccttta tctcctatgc gctactttga acttgatgga attcccaatg acatacagaa
      121 tattatttgg tggtaaatgg gcaatgatat ttcttggacc aaagatgaaa cattgctagt
      181 tcatgtggat ttggtggaat ccctaccaca atttcaatat cacaagatga aaattgacca
      241 gtattgcaag aatataaaat agcaagagat ggaagtgaat acgacagctc cacaagagaa
      301 atgttttcta aacaagctta caatatacga agaaattgaa aatatacttt caacaatact
      361 gaaaaaactt ttttggaaca agctgaagaa tattttggca caacagtcat tccagaatca
      421 gaaaataatt ttgattgtcc attctgtgat aagctacgat attataactg gaatccagaa
      481 gatgatgaaa aactacaaga cagcaatgat ttaatattca attatgtgtc aaacgacaac
      541 gggaaaaata caatatattt tggcagtgat tgaagttttg aagcaaagtg aagttgggat
      601 gaatgaactt gaactcgagt ttttggaaaa gatgaaaaca ctgtcatagt atcatcaaac
      661 aaggccaacc atatttatga tagatatatt atcaccaatc aagatgaaaa taatctgaac
      721 acgatattag aagtcataca aagataaaac aaataatttc aaatttccta ttgaaatatt
      781 ttatcattta agtattaact taataagtat agtttgtcat acataaacga caaatttgtt
      841 tagttcctaa tttttttcca aatgaaaatc acaataaaca cagctaaatt aaatgatatc
      901 gtggatatca gtagtagatt tgtagccaaa aacgcgacat taccaatttt acaaaacatc
      961 tatctaaaag catctataga caatctgatt gtaagagcta ctgatatgga aaaatatatt
     1021 gaaatagaaa caccttgtga cataaagttg gaatgagcta ttacgataaa tgctaaaatg
     1081 ttttctgata ttatcaaaac tatagaagat gagcaaatag aaattagcac tgatccacaa
     1141 acaaatacta tgatcatcaa aacaagaaaa gataattttg aaataaatgg aatatcagca
     1201 aacgaatatg ttgctttacc agatgttcca caagaaaaca caattacatt agatactcaa
     1261 tcactttctg atggtataga aaaagtagag tattctgtaa cagaaaaaaa cttcagccca
     1321 gtacttactt gagtcctaat gaaatcaaaa gaagaaaact gaagcaaaaa actagttttt
     1381 gtaggtacag attcatttag actaactgaa tacaaaacca acagctctat ccaatgagat
     1441 gacttttctt tgatcattcc aaagatgtcc atcattgata tcaaaaaaat atctgatttt
     1501 gcaaaagaaa aagaatcaga aaatgtaatc gtaaaatatt caaacaactt agttgctttt
     1561 gaatttgaaa taaacgatat gaaaatatta gcaacatcat tactaattca atgaaatttc
     1621 ccagactacg aaaaagagga aattatgcca acaaatttca acacaaaact aattatcaat
     1681 aaagacgact gtgaaaaagc tatcaaaaag atatgaattt tgaccagaga cataaacaac
     1741 tacattgaat tagcaataga caacggagaa gctatagtat cttcaggtaa aactgataaa
     1801 tgaatgtgaa aaaccaatat tccaatactt atggaatgag aatctgttag ctttggagta
     1861 aatggtagat atatcacaga tttcatcaga acaatggaaa gcgatgaaat cgtgttcaat
     1921 atagtggaca accaaagacc aataatatta caaaataaaa acaatacaaa ttacaattgt
     1981 gtaattagac cattattaaa cggataaaaa tctaaaatta aattatgatt acaatattaa
     2041 ttattttatt cgtactcgct atgatcaatt gtaggcaaag gcaatgaaca aaaaatgagc
     2101 caaaggagat aatcttacct tggctttttt tgattgaatt aaatatactt ttgtatatat
     2161 tcaagatgga atttatgata aaataataaa aaatgaaaaa agcatttacc ctaatagaaa
     2221 ttatagtaat agtagggata ctagcgatat tattatgaac ttcttctttt ttaagaaata
     2281 gaaatgaaga tgaaaaaata aaatattgaa aagaatgttc aaattatatt tttcaagaag
     2341 tcctaaacca agataacaat attaaaaaaa ataaaacaat taactcctga gacgaaatct
     2401 tactaataac atgaatacaa ataaaaaaag aaaataaaga tagttactta gtaagtatga
     2461 atacattcaa tgaaaaatca gaacttaatt ctcaaaacac actaataaat gataagtgat
     2521 catgtacagc tgaccacata tcaaataaaa ataattatat tataaataca gatgatgact
     2581 ttatgataag aatacaaaaa aataataact ttgcctacaa aactacaata tcagtttgcg
     2641 attttggatg aaataattgt attgaaattt caaaaataga atacaatcaa gccagtaata
     2701 aatttgattt aaaatcttgt tctttaataa ataatgaatg actttgtgaa gaatggaaaa
     2761 aatagaaaaa gaattaattt tttataaaat catggattat ccaatcccaa aagataaaga
     2821 acttttttat tttgagaaaa aaatagagca aaagaaaaat ccaagtagtt gattttcacg
     2881 ggatgagaaa aataaatata tggatacata tataaaaaga ataaacaaat tttgattttt
     2941 gtataaatcc ttaccatttg taaaagaaat ttttttatgt aattcaatat ctttcaactc
     3001 actcaaacga gattcagata tagatctatt tatagtaaca aaatccaata gtatacgaag
     3061 agccagattt ttctcagccc tattttttaa gat
//
LOCUS       DAOSID010000085         3007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_28499_length_3007_cov_17.712060, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000085 DAOSID010000000
VERSION     DAOSID010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28499_length_3007_cov_17.712060"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..>1400
                     /gene="truD"
                     /locus_tag="PK674_03330"
     CDS             224..>1400
                     /gene="truD"
                     /locus_tag="PK674_03330"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HOG15596.1"
                     /translation="MLFQFKSKPEDFIVKEELGFTPSGKGDVFFVNFQKTEKNTMDII
                     EHLCYELKLKRNDLGIAGLKDKNGITEQRISIYKKILNQIGGEEKFLEVLGQKTIILD
                     TTWHHEPLAIGKNAGNYFKIKLQAKSVISPEIKEKIENNIKKIQQNGFPNCFGKQRFG
                     KGYRNFFRAKEVFESNSTNNPSKLNKKNPQSEFEIRFKLQAYASMYFNEYTMNRRKRG
                     QIFLGGDIMVDNNSAFGTRVGIYGEGKVMLFNYAKCKKEFEGQNLIQPYYISGETIDL
                     KNNKERIPTGPMLGFNMLTPPLESKSYIKDSELLKNTNYENLGMNVAKKYKIYGFRRP
                     LRTIPKNLKYSREENKKDKFKKDLILSFSLPTGSYATIFLGTIFDGIDEKTIIENGLE
                     "
     assembly_gap    1401..1500
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1614..>3007)
                     /gene="gatA"
                     /locus_tag="PK674_03335"
     CDS             complement(1614..>3007)
                     /gene="gatA"
                     /locus_tag="PK674_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=25
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOG15597.1"
                     /translation="NLFMSKQNLVEEYKNKIENSIKNKENIFLRLENNIECKNPDGIL
                     GGMIVGIKDNIMTKGNISSCGSKMLANYVAPYTATCIENLQKNGGIVIGKTNMDEFAM
                     GSTNETSFFGTPINPFGTNRVAGGSSGGSAVAVAKDLCMVALGTDTGGSVRQPAAFCG
                     LVGLKPTYGRISRYGVQSMASSLDQVGTFTKTIDDAETLLKAIMGFDLKDSQSDKRAD
                     ELLKSTKKINEYKIALPKQAFGEGLDPKIKARILEVVEKLRSKGVQIDEIDLPVLEYA
                     LPIYYTLMPAEVSTNLSRFDGIRFGLQKNTMDYDNIQDYYSAIRSEGFGEEVKRRILL
                     GSYVLSSSNYEGYYLKAQKARQKLTQDIDNIFQKYDLILTPTTPEVAWKIGEKINDPL
                     KMYLADIYTVPANLAGLPAISIPVGTVEDELADKSGLNFVMEQMPVGLHLMANKWKED
                     DLFVVGREIERLY"
BASE COUNT         1087 a          511 c          406 g          903 t
ORIGIN      
        1 attcttccct tgggggaaga gttgctctat cagtacaaac acagaatcct tccccaacgg
       61 ggaagttgct tcccgtcccg tagtcctacg ggacgggata gttgggggtt agatccccct
      121 taatccccct tattaagggg gattttaccc ccacctagct ccacaaacag cctccccaag
      181 gggaggaatt taatcctcga actttaagaa ctttacaaac ttgatgctct tccaattcaa
      241 atccaaacca gaagatttta tagtaaaaga agaattatga ttcacaccaa gttgaaaagg
      301 tgatgttttt tttgtaaact ttcaaaaaac tgagaaaaac acaatggaca taattgaaca
      361 tttatgctat gagctaaaat tgaaaagaaa cgacttagga atagcggggt tgaaagacaa
      421 aaacggaatc acagaacaac gaataagcat atataaaaaa atattaaatc aaattggatg
      481 agaggaaaag tttttggaag tattatgaca aaaaacaatt atattagata caacatggca
      541 ccatgagcca ctagcaatag gtaaaaatgc atgaaattac ttcaaaataa agctacaagc
      601 aaaatcagta attagtcctg aaatcaaaga aaaaatagaa aacaacatca aaaaaataca
      661 acaaaattga ttcccaaact gctttggtaa acaaagattt ggtaaatgat atagaaattt
      721 ttttcgtgca aaagaagttt ttgaaagcaa ttctacaaat aacccgtcaa aactaaacaa
      781 aaaaaatcca caaagtgagt ttgagattcg tttcaaacta caagcatacg ccagcatgta
      841 ctttaacgaa tatacaatga ataggcgaaa aagatgacaa atatttttat gatgagatat
      901 tatggtggac aataatagcg ctttttgaac aagagtagga atttatggtg aatgaaaagt
      961 gatgttattc aactatgcaa aatgtaaaaa agaatttgaa tgacaaaatt taatacaacc
     1021 atattatatc agcggagaaa ctatagactt gaaaaacaac aaagaacgaa ttcctacatg
     1081 accaatgctt ggctttaata tgctaactcc tccattagag tcaaaatcat atatcaaaga
     1141 ttctgaactt ttgaaaaata ctaactacga aaatctggga atgaatgtgg caaaaaaata
     1201 taaaatttat ggttttagaa gacctttgcg aacaataccc aaaaacctga aatattcgcg
     1261 agaagaaaac aaaaaagaca aattcaaaaa agatctaatc ttatcatttt cattacctac
     1321 aggaagctat gctacaatat ttctatgaac aatttttgat tgaatagatg aaaaaacaat
     1381 catagaaaac tgattagaaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 aaaaaatttt tgataaatga attttagaat atctaaaatc tcccaaagca aaagaaacaa
     1561 ccacacaaat caacaaacat ttggaacaaa taatagatga agaagacaag tacttaatat
     1621 aatctctcta tttctctacc tacaacaaac aaatcatctt ctttccattt atttgccatt
     1681 aaatgaagtc ctactggcat ttgttccata acaaaattaa gtcccgattt atcggcaagc
     1741 tcatcttcta cagtaccaac aggaatagaa attgctggta atcaagctaa attagctgga
     1801 actgtatata tatctgctaa atacattttc aaaggatcgt taattttttc tcaaattttc
     1861 caagcaactt ctggtgttgt aggagtcaaa attaaatcat atttttgaaa aatattatca
     1921 atatcctgag ttaatttttg tctagctttt tgggctttca aatagtatca ctcataattt
     1981 gagctactaa gaacataaga accaagcaaa atcctccttt ttacttcctc tccaaatcac
     2041 tctgatctga tagcagaata gtaatcttga atgttatcat aatccattgt atttttctgt
     2101 aaaccaaatc taatcccatc aaatctagat aaattagtac taacctctgc tggcattaaa
     2161 gtataataaa taggcagagc atactctaaa actggcaaat ctatttcatc tatttgtact
     2221 catttagaac gaagtttctc taccacttct agaattctag ctttaatttt gggatctaat
     2281 cattctcaaa aagcctgttt tggtaaagct atcttgtatt catttatttt tttagtagat
     2341 tttagcagtt catcagctct tttatctgat tgagaatcct ttagatcaaa tcacataata
     2401 gcttttaata aagtttctgc atcatctatg gttttggtga aggttcaaac ctgatccaaa
     2461 ctacttgcca tagattgaac cccataccta ctgattctac cataagttgg cttgagtcaa
     2521 acaagtccac aaaatgctgc tggttgacga acacttcctc ctgtatcagt tcatagtgct
     2581 accatacaaa gatcttttgc cacagctaca gctgatcatc aactagagcc tcctgccact
     2641 ctatttgttc aaaatggatt tatcggcgtt ccaaaaaaag aggtttcatt agtagatcac
     2701 atagcaaatt catccatgtt tgtctttcat atcacaattc atcaattttt ttgtaaattt
     2761 tctatacaag tcgctgtata tggagctaca taatttgcca acatctttga tccacaacta
     2821 gaaatatttc attttgtcat aatattatct ttaattccaa ctatcattca tcataaaatt
     2881 cagtcaggat ttttgcactc aatattattc tccaatctca aaaaaatatt ttctttgttt
     2941 ttgatactgt tttctatttt atttttatat tcttccacaa gattttgttt agacataaaa
     3001 agatttt
//
LOCUS       DAOSID010000086         2989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_28739_length_2989_cov_18.753920, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000086 DAOSID010000000
VERSION     DAOSID010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28739_length_2989_cov_18.753920"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PK674_03340"
     CDS             <1..183
                     /locus_tag="PK674_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HOG15598.1"
                     /translation="GKWRFSEKQLLILVGLGGFVGSILGMQIFRHKTIKSKFLYWFRG
                     IVFIQLALLVVLMIKG"
     gene            303..653
                     /locus_tag="PK674_03345"
     CDS             303..653
                     /locus_tag="PK674_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="four helix bundle protein"
                     /protein_id="HOG15599.1"
                     /translation="MSNTFENLEVWSLGRNLVNKLGKIFYDSSFKNYGFQDQIMRAAI
                     SITNNIAEGRERPSNKDFIKFLYYSKGSAGEVRSMLYLALDFGFIDQEMFEELKNDCT
                     EITIKLYYLIKSLI"
     gene            729..1394
                     /gene="rsmI"
                     /locus_tag="PK674_03350"
     CDS             729..1394
                     /gene="rsmI"
                     /locus_tag="PK674_03350"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOG15600.1"
                     /translation="MLYIIPTPIGNREDITLRALRLFKELEIFICEDTRTFKKLLSMY
                     EIEYSNKEFYSLTSFTDKGKLNFYLNLLKSKDVGLVSEAGTPGLSDPGKSLIQLCNEN
                     NIIYTVLPGANALVPSVVSASFDTSSFIFLGFLPQKKGRQTALKKAIQSDVPSFFYES
                     VHRFEKLLCELRDLGFNGKIFVAREISKIYEQIFNGNLDEIIDLVKSKKLPIKGEFVI
                     GLY"
     gene            complement(1401..2876)
                     /locus_tag="PK674_03355"
     CDS             complement(1401..2876)
                     /locus_tag="PK674_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15601.1"
                     /translation="MSKSKTNNLTPKEQAKKSGEAFLKAGKNILQTTKGAVKTVWNTG
                     EGLYHLVEAGDLAIHNKLGKSSNKIVNFTKRNIIKILLATSILTCSGAKVYQHQKDKD
                     PNKIELYEDLDKNIIDLKNIGYKIKTPNSLRRKNLDLLKGRDIDSLKGEKILDSLKLR
                     KVGDLYSYYLGYGGEPVEIGDTIIFKPNEIIALLYQRKFNRKNRKGFTVAKEFYDKTV
                     SNINFNNVVPSSLDLMTKNINDAIKEVNENFDREKFGKERLKNNKSKINLFKKICDKI
                     DQKCLIAYGMTEIMPSRDGELNKEVLDFLLKNGGERFVMNLPAVNDDYASFGFYQLTS
                     KAVFDTGDEKKGGVSKMNLYMPKEHKVPGSVIMLQGQDHHKAAYLFAMYNLYTLVFKA
                     DSEMVKALELLANQQDNNDLTQLIAIMHNLPKNGQPFLYEWYKLNTDKNYLHQKTHNK
                     KGKPYNYDLNGNKKIDLYESFVSPKTSHNYGKKTYYNRKSL"
BASE COUNT          997 a          365 c          391 g         1236 t
ORIGIN      
        1 tgaaaatgga gatttagtga aaagcaatta ctgattttgg tttgattagg agggtttgtg
       61 ggttcaattc tatgaatgca aatatttcgt cataagacta taaagtctaa gttcttatat
      121 tggtttcgat gaattgtatt catacaactc gctctactcg ttgtactcat gataaagtga
      181 taaatacgaa cttcgttcgt gataaaatgt taaaatgatg aaaacagatt tcgttcgtga
      241 taaagtgata aaatgttgaa gcaataaaat aataagcttt tttatttaca taaatagttt
      301 ggatgtccaa tacatttgaa aatttagaag tttggagttt gtgaagaaat ttagttaata
      361 aactttgaaa aatattctac gatagtagtt tcaaaaatta ttgatttcaa gatcaaataa
      421 tgagagcagc aatttccatt acaaataata tagcagaatg acgtgaaaga ccttcaaata
      481 aagattttat taaatttctt tattattcca aatgatcggc gggagaagta agaagcatgc
      541 tatatttagc attagatttt tgatttattg atcaggaaat gtttgaagaa cttaaaaatg
      601 attgtacaga aataactatt aaattatatt atcttataaa atctttgata taaattttat
      661 tttagtacaa tttatcactt tatcatttta tcgcgagcaa agcgagcatt tatcatttta
      721 ccatttctat gctgtatata atcccaacac cgatatgaaa tagagaagat attactttga
      781 gagctttgag attattcaaa gaattagaga tttttatatg cgaagatact aggactttca
      841 aaaaactttt atcaatgtat gaaattgaat attcaaataa agaattctat tcactgacca
      901 gttttacaga taaaggaaaa ttaaattttt atttgaattt attgaaaagc aaggatgttt
      961 gattggtttc tgaggctggt acaccttgat tatctgatcc agggaaaagt ttgatacaac
     1021 tatgcaatga aaataatata atttatactg tattaccttg agcaaatgct ttggtaccaa
     1081 gtgtagtttc tgcttctttt gatactagta gctttatttt tttaggattt ttaccacaaa
     1141 aaaaaggaag acaaacagca ttaaaaaaag ctattcaaag tgatgttcca agtttttttt
     1201 atgaatcagt tcatagattt gaaaaacttt tgtgtgaatt aagggatttg tgatttaatg
     1261 gaaaaatctt tgtagctaga gaaattagca aaatatatga gcagattttt aattgaaatt
     1321 tagatgaaat tatagatctt gtaaaaagta aaaaactccc aattaaatga gagtttgtta
     1381 taggactgta ttaaaaatta ttataaactt tttctgttgt aataagtttt ttttcaataa
     1441 ttgtgggaag tttttgggct tacaaaagat tcatacaaat caattttttt gtttcaattt
     1501 aaatcataat tatatggttt tcatttttta ttatgtgttt tttgatgaag ataatttttg
     1561 tctgtattta atttatacca ttcataaaga aaaggttgac cgtttttcgg taaattatgc
     1621 attatggcta tcagttgtgt taaatcatta ttatcttgtt ggtttgctaa caattctaaa
     1681 gcctttacca tctccgaatc agccttaaaa actaaagtat ataaattata catagcaaat
     1741 aaataagctg ctttgtggtg atcttgtcat tgcaacatta tcacacttca aggtactttg
     1801 tgttcttttg gcatatataa attcatttta gatactcatc cttttttctc atctcatgta
     1861 tcaaatactg cttttgatgt aagttgataa aatccaaatg atgcataatc atcattcaca
     1921 gcaggtaaat tcatcacaaa tctttctcat caatttttga gtaaaaaatc taatacttct
     1981 ttatttaatt ctccatctct acttggcatt atttccgtca taccatatgc tattaaacat
     2041 ttttgatcaa ttttatcaca aattttttta aataaattga ttttactttt attattttta
     2101 agtctttctt ttcaaaattt ttctcgatca aaattttcat ttacttcttt tatagcatca
     2161 ttaatatttt ttgtcattaa gtctaagcta gatgggacca cattattaaa atttatatta
     2221 cttactgttt tatcataaaa ttcttttgct acagtaaatc actttctatt tttacgatta
     2281 aattttctct gatataatag agctattatt tcattgggtt taaatattat agtatctcat
     2341 atttcaacag gttctcaacc atatcataaa tagtatgaat ataaatctcc aacttttctt
     2401 aattttaatg aatccagtat tttttctcat tttaatgaat ctatatccct tcattttaat
     2461 aaatctaaat tttttcgtct taatgaattt ggtgttttta ttttatatcc aatatttttt
     2521 aaatcaatta tatttttatc taaatcttca tataattcta ttttgtttgg atctttatct
     2581 ttttgatgct gataaacttt tgctcatgaa catgtaagaa tgcttgtagc aagcaatatt
     2641 tttattatat ttctttttgt aaaatttaca attttattag aagattttca taatttatta
     2701 tggatggcta aatctcatgc ctctacaaga tgatataatc attctcctgt gttccaaact
     2761 gtttttactg ctcactttgt tgtctgaagt atattttttc aagcttttaa aaaagcttct
     2821 catgattttt tagcttgttc tttaggcgtt aaattgttag tttttgattt gctcatctgg
     2881 aatattttat ttaaaataaa aacattttct ttaattttta tgctcttctg tgttttgttt
     2941 atatttattg tttttcaaaa tttcttttgc caatttttct aaatcaagt
//
LOCUS       DAOSID010000087         2956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_29132_length_2956_cov_13.332299, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000087 DAOSID010000000
VERSION     DAOSID010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29132_length_2956_cov_13.332299"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PK674_03360"
     CDS             complement(<1..459)
                     /locus_tag="PK674_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15602.1"
                     /translation="MITSKECKDLLQKQFQKDFSQKQNVSQLMTKSRGENRSDCFSYF
                     GNIGNINSYLENIQNNEYMISRGITESDANRAKTIEKQAHASAKVNDNNPSSFGGIKV
                     AGMFLLYLFLSVIIIRIFSRLMRYVLRRGYAFLNNHRMVYMKVLVPRGDSK"
     gene            complement(689..1033)
                     /locus_tag="PK674_03365"
     CDS             complement(689..1033)
                     /locus_tag="PK674_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15603.1"
                     /translation="MATDQKVKEFSSRSYDERLEISMNIITNLKDRGNTQAQELYDKI
                     SQMEKVPDAVINAIFQDFYDSVERIQREKIQSELHQFTKAGAYMEQLREKERQERKRE
                     NADGILDMIDDL"
     gene            complement(1049..2359)
                     /locus_tag="PK674_03370"
     CDS             complement(1049..2359)
                     /locus_tag="PK674_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15604.1"
                     /translation="MTLETKQQDQLDLKMKFEGDLRRVDSEKVEFPKTEQMVNPVKVG
                     VLNVEMLDLEDLQVWGRRDKIAPIDKQEIVSGVNLLTEPYKSEVSDLIKANNVKGLQA
                     YLNEKIKNNLSISQNLKEALAQKGISFRNNQILEDGKFGPQTLEALKCFIRECDGDRP
                     VKEPVDEPVDKPVESSEKEKGWDFSWILQEMADKIEDVEDTGAINNKDRSDRNTPSSV
                     LSEAFKVDPDKSKVILSTRGGDVNTPCCDKFDSQNYCEYDRKTGQITVKCGYYSYPLT
                     GFALKIPPLNLGKDGLPMEGDLTNRKTIKNFADIANTLNMLKKTCVFTNKGAIEFTTA
                     LRNFRANIGGKWLYNYQLELNNAVLPIADTKEITTQALEKKWLECGAKFTVEEGKKLA
                     GLLNAMKLDMGKITRYDDIATPIWKDGVDKETQEYIKTYLKKPI"
     gene            complement(2655..>2956)
                     /locus_tag="PK674_03375"
     CDS             complement(2655..>2956)
                     /locus_tag="PK674_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=25
                     /product="3'-5' exonuclease"
                     /protein_id="HOG15605.1"
                     /translation="DLGFLSECVYSCQGFWLDNDVICTRKLANRLLPDLDRKNLGSIC
                     DYMGLVNDSAHRAMGDTEVTVKVFKNFLKLLKNNGIDDKLEIIKFQDKSIRDCFF"
BASE COUNT          961 a          517 c          313 g         1165 t
ORIGIN      
        1 tttactatct catcttggga caagtacttt catgtaaacc attctatgat tgttcaaaaa
       61 agcatatcat cgccttagca catatctcat aagtcttgaa aaaatacgaa ttattataac
      121 tgacaaaaat aaatataata gaaacattca agctacttta atcccaccaa aagatgaagg
      181 attattgtca tttaccttgg cagaagcatg tgcttgcttt tctattgttt ttgccctatt
      241 tgcatcgctt tcagtaatcc ccctgcttat catatactca ttattttgta tgttttctaa
      301 ataagaatta atatttcata tattaccaaa atatgaaaaa cagtcacttc tattttctca
      361 tcgagattta gtcatcaatt gagatacatt ttgtttctga gaaaaatctt tttgaaattg
      421 cttttgtaat agatccttac attctttgga agtaatcatg ctttatgatt ttttgaacaa
      481 ataaaatttc gtaccttctt tattatattc aaaaaaaaat atatagctaa aatttggact
      541 aaaaatgtct tcaaagactt gtctttttga aaattagtta taagatatat ttaattcata
      601 cataaatttt aaatataaaa aaagacccct taaaaaatac aggaagtctt ttttagtata
      661 taatattaat gttacaattt attataactt atagatcatc tatcatatct aaaattcaat
      721 ctgcattctc tctttttctc tcctgccttt ctttttccct aagttgctcc atgtatgctc
      781 atgctttagt aaattgatgt aattcagatt gaattttctc tctctgaata cgctccactg
      841 aatcataaaa atcttgaaat attgcattta tcactgcatc tggaactttt tccatctgtg
      901 atattttatc ataaagctct tgtgcttgtg tatttcaacg atctttcaaa ttcgtaataa
      961 tgttcataga gatttccaat ctctcatcat acgatcttga actaaactct tttacttttt
     1021 ggtctgttgc cattgttagt atcaaaattt aaatcggttt ttttaaataa gtttttatat
     1081 attcctgtgt ttctttatcc acaccatcct tccatattgg agtagctata tcgtcatacc
     1141 gtgtaatctt tcacatatct aatttcatag catttaataa tcatgctaat ttctttcatt
     1201 cctcgactgt aaatttagca ccacattcta gccacttttt ttctaaggct tgtgttgtaa
     1261 tttcttttgt atccgctatt ggcagcactg cattattcaa ttctaattga tagttatata
     1321 accattttca tcatatattt gccctaaaat ttcttagagc cgttgtaaat tcaatagctc
     1381 atttattagt aaaaacacat gtctttttta acatattaag tgtattagct atatcagcaa
     1441 aatttttgat tgttttacga tttgtcaaat ctcattccat aggcaatcca tcttttcata
     1501 aatttagtgg aggaattttt aatgcaaatc atgttaaagg atatgaataa tatccacact
     1561 ttacagttat ctgtcatgtt tttcgatcat attcacaata attttgtgaa tcaaatttat
     1621 cacaacatgg cgtattaaca tctcatcacc gtgttgataa tatgactttt gacttatctg
     1681 gatccacctt aaaagcttct gaaagaacag acgatggggt atttctatct gatctgtcct
     1741 tattatttat agctcaagta tcttctacat cttctatttt atccgccatc tcttgtaata
     1801 tccatgaaaa atcccatcat ttttcttttt ctgaactctc aacaggttta tctactggtt
     1861 cgtctactgg ttctttcact ggtctatcac catcacattc tcttataaaa cattttaatg
     1921 cctctaatgt ttgagggcca aattttccat cttcaaggat ttgattattc ctaaaagaaa
     1981 ttcccttctg tgccaaagcc tctttcaaat tttgagaaat acttaaattg tttttaattt
     2041 tttcattcaa ataagcttgc aatcatttta cattattagc ttttattaaa tcagagacct
     2101 cagatttata tggctctgtc aagagattaa caccagatac aatctcttgt ttatcaatag
     2161 gggcaatttt atctctccgt ccccatactt gtaaatcctc taaatcaagc atttctacat
     2221 ttaaaacacc aacttttact ggatttacca tttgttctgt ttttggaaac tctacctttt
     2281 ctgaatcaac tcttcttaag tctccttcaa atttcatttt tagatccaac tgatcttgtt
     2341 gttttgtttc taaagtcatt tttgtttaat taaagaataa aaaagttttt ttgacaattt
     2401 aattatacac acaaaaaaaa acttgtcaaa tttttgacca ctttttttgc tagctcaaaa
     2461 gtgcagtttc aacaaagata tgaaaaaagt cgaaaaaaaa caaaaaaaat attttttttt
     2521 agaaaatttt ttaatctaaa ccaaaaatat ttttttattt caaaaatatg acaaaatatg
     2581 tttttatttc aaaaatgtgc caatatacaa aaatcatcat ttttcttaaa gaaatatata
     2641 ttagagccct tactttagaa aaaacaatct ctgatacttt tatcttggaa ttttattatt
     2701 tccaacttat catcaattca attatttttt aacaatttta agaaattttt gaaaactttt
     2761 acagtaactt cagtatctca catcgctcta tgagcagaat catttaccaa tcacatataa
     2821 tcacaaatac ttcataaatt ttttctatca aggtctggaa gcaatctatt tgccaatttt
     2881 ctcgtacata taacatcatt gtccaaccaa aatcattggc aagaataaac acattcagaa
     2941 agaaatcaca aatcaa
//
LOCUS       DAOSID010000088         2943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_29296_length_2943_cov_10.726108, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000088 DAOSID010000000
VERSION     DAOSID010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29296_length_2943_cov_10.726108"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="PK674_03380"
     CDS             complement(<1..1072)
                     /locus_tag="PK674_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOG15606.1"
                     /translation="MTFRSKGLDYLKFSITLFLLFVFVLVIVYADKFQKGPDTLYVNT
                     VAFAQTNSDEQHLINLEYVTAEIGEDNKGVINYIVQPGDTLSKIASTFGTTVSHIKKT
                     NNINGPIQPNQKLIITDESEGLIYTIDGTQNILVFANRYGLNVEDLMSLNYIQDETEM
                     LQDGQEIFVPITLEKAYDIGMMERPKPVYKPKTTVTYKPTITKPTVSSYVVSSRTPSV
                     ASIGDYSSSSILSSWVFNKNINNKFYAGHCTWYIAAITPQIFPYLDDTSQARPFGGNA
                     NQWYANAKSAGFSVGSKPVAGSIVVYRRGGGGYAGAGHVAKVVSYNAGAGTMVVEEMN
                     GSKKFVVQRRTDRVDNPNIIGYI"
     gene            complement(1092..1181)
                     /locus_tag="PK674_03385"
     tRNA            complement(1092..1181)
                     /locus_tag="PK674_03385"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1145..1147),aa:Ser,seq:tga)
     gene            1338..1823
                     /gene="def"
                     /locus_tag="PK674_03390"
     CDS             1338..1823
                     /gene="def"
                     /locus_tag="PK674_03390"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="peptide deformylase"
                     /protein_id="HOG15607.1"
                     /translation="MINLKKFYPVQTGSDNKILRTKSKKIDSINQDILEFAETLMELM
                     YEYDGVGLAAPQLGRNIRMIAITFWKENKKGSERIDEDIMINPEITEKSKEMSISKEA
                     CLSVPNVIGKVKRHQNIVVEYLDIFGRKKKKKLRNYNAFIVQHEIDHLDAILFVDKLI
                     K"
     gene            2221..2532
                     /locus_tag="PK674_03395"
     CDS             2221..2532
                     /locus_tag="PK674_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="RNHCP domain-containing protein"
                     /protein_id="HOG15608.1"
                     /translation="MKKINESFICINCKKEIPLASKTCRNHCPYCFVSLHVDGDIPGD
                     RNTTCHGKMYPREYFIANGNMKILFECEKCHKQHRNKRAKDDEVENLDKFISLYKKSL
                     L"
     gene            complement(2573..2643)
                     /locus_tag="PK674_03400"
     tRNA            complement(2573..2643)
                     /locus_tag="PK674_03400"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2609..2611),aa:Arg,seq:tcg)
BASE COUNT         1052 a          490 c          430 g          971 t
ORIGIN      
        1 aaatatatca aattatattt ggattatcta ctctatctgt acgcctttgt actacaaatt
       61 ttttacttcc attcatttcc tctacaacca tagttcaagc tcatgcatta taactaacaa
      121 cttttgctac atgtcctgca cctgcatatc atcatcctca tctacgataa acaactatgg
      181 atcatgcaac aggcttacta ccaacactaa atcaagcaga ctttgcattt gcataccatt
      241 gatttgcatt tcaaccaaaa ggtctagctt gagaagtatc atctaaatat gggaagattt
      301 gaggcgttat ggctgctata taccaagtac agtgtcatgc ataaaactta ttattaatat
      361 ttttattaaa cacccaacta gataatatag aagagctaga ataatctcaa atagaagcaa
      421 ctgagggagt cctactactt acaacataag aagaaactgt tggctttgta attgtaggtt
      481 tgtatgtcac cgtagttttt ggtttgtaga caggtttagg tctttccatc attcaaatat
      541 cataagcttt ttccaatgta ataggaacaa atatttcctg tcaatcttgt agcatctccg
      601 tttcatcttg aatatagttt aacgacatta aatcctccac attcaatcca tacctgtttg
      661 caaacaccaa aatattttga gttcaatcta tagtataaat caatccttct gactcatcag
      721 taataatcag tttttgattt ggttgtatcg gtcaattaat attatttgtt tttttgatat
      781 gagaaacagt agtaccaaaa gtactagcaa tttttgataa agtatctcca ggctgaacta
      841 tataattgat tactcatttg ttgtcttctc atatttctgc agtaacatac tctagattga
      901 tcaaatgctg ttcatcagag tttgtttgtg caaacgcaac cgtatttaca tacaatgtat
      961 cgggtcattt ttggaatttg tccgcatata ctataaccaa tacaaacaca aacaataaaa
     1021 ataaagtgat tgaaaatttt aaataatcta atcctttgct tctaaatgtc atatagtaat
     1081 ttggtgataa atggcggaga gggtgggatt tgaacccacg gtccgtgtga acgaacaaca
     1141 gttttcaaga ctgccgcatt cgaccactct gccacctctc cacaggttta cttattacac
     1201 agagtatctg cacatttgtc gaacacgatc acagtaataa tatttatttt gaattgattt
     1261 tcaagtatta aaaaataaac tacaactcag tactccaagt gtaattattt ttactataaa
     1321 gtttgttttt ctaaaaaatg attaatttaa aaaaattcta tcctgtacaa acatgatcag
     1381 acaacaaaat tttaagaaca aaaagtaaga aaatagattc tataaatcaa gatattttgg
     1441 aattcgcaga aactttaatg gaactaatgt acgaatatga ttgagtatga ctagcagccc
     1501 cacaattatg aagaaatatt aggatgattg ctattacttt ttggaaagaa aacaaaaaat
     1561 gatcagaacg aatagacgaa gatatcatga taaatccaga aattaccgaa aaatccaagg
     1621 aaatgtcaat ctcaaaagaa gcttgtttat cagtccctaa tgtaatttga aaagtaaaaa
     1681 gacaccaaaa tatagtggta gaatatttag acatcttcgg tagaaaaaag aaaaaaaagc
     1741 taagaaacta caatgcattt atagttcagc atgaaatcga ccatttggat gctatattat
     1801 ttgtagataa attgattaaa taagaagtca aagcacacaa accccaaaga agcatatcgc
     1861 caataaacca tccggtgcaa aaattctata gtttttttat tcaaaaagtt attttctact
     1921 acttcaaagc tcaaagcatc ttctattatt tcatatttat attattctga gaaactaagc
     1981 tcttttcata aaaccaaaaa atattcatct cacaatttca acaactctca tcataatttt
     2041 atttccaaaa aaaacatctt tcaaaaaatt cttaaatcgg tataatatag ttaatcagtt
     2101 taatacgacc aaactgtgaa tgaacaagca ctataatatt atatcattgc taaaatgact
     2161 aaaaatatat tccttgtata aacaaggctt tggaatacaa catttatccc caaattagat
     2221 atgaaaaaaa taaacgaaag ttttatttgt ataaattgca aaaaagaaat tcccctagct
     2281 tccaaaacat gcagaaatca ctgtccgtat tgttttgttt ctttgcatgt agattgagat
     2341 attccttgag atagaaatac tacttgtcat gggaaaatgt atcctagaga gtattttata
     2401 gccaattgaa atatgaaaat tttgtttgaa tgtgagaaat gccacaaaca acatcgaaac
     2461 aaaagagcta aagatgatga agtagaaaat ttggataaat ttataagctt atataaaaaa
     2521 tccctacttt aatcagggat ttttctattt tgatgttttt agtttttcaa atcagggaca
     2581 gggggaatcg aacccacagt gacggttccg aagaccgttg tgttaccatt acaccatgcc
     2641 cctacttagt aaatgagttt gtaaagttta taaagtttat aaagcctgcc acgcagtact
     2701 gcgtggcggg ctacgtggcg gagttcgtaa agttatagcc tcttaataag ggggaagggg
     2761 ggattttgtg ataaattaat gttttcattt aatccccctt aatccccctt gaaaaggggg
     2821 ataaccatcg acctgtaact ttataaactt atttgttcac taggattttg attattcaat
     2881 tttccaactc ttttaatact tcttcttttc agttgcttgg aattttatcg tttgttttta
     2941 tga
//
LOCUS       DAOSID010000089         2921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_29566_length_2921_cov_15.819609, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000089 DAOSID010000000
VERSION     DAOSID010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29566_length_2921_cov_15.819609"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(526..1221)
                     /locus_tag="PK674_03405"
     CDS             complement(526..1221)
                     /locus_tag="PK674_03405"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HOG15609.1"
                     /translation="MKKTLILFSVLGLVLLFAGCTSLEQVNRNRLELTSQSRGSKITA
                     NQQQGQGGGNAKRGQTVRGGGQQGIGRNSSATGINHMNLVVDTGLDLTPEQISELQFI
                     IEEEKLAQDIYQVMYDQRGDKRFNNIIKGEINHQSVIATILEAYGLENPTEGMSAGEF
                     KNTELQQLYDDLIVKGKTSSAEAIQVGILVEEKDIVDLENMMPLFKNHPSIIQGLQIL
                     LNGSKNHLQAFKR"
     gene            complement(1397..1636)
                     /locus_tag="PK674_03410"
     CDS             complement(1397..1636)
                     /locus_tag="PK674_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15610.1"
                     /translation="MRKQIILGSLFMLIGAVLIYYSAQLVDLLGKNARAEKYLGGTRN
                     AIVLFGFGIIVLGVLYMFGVLDMGSPLKSDSVGTF"
     gene            complement(1674..1747)
                     /locus_tag="PK674_03415"
     tRNA            complement(1674..1747)
                     /locus_tag="PK674_03415"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1710..1712),aa:Gly,seq:tca)
     gene            1939..>2921
                     /locus_tag="PK674_03420"
     CDS             1939..>2921
                     /locus_tag="PK674_03420"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="IMP dehydrogenase"
                     /protein_id="HOG15611.1"
                     /translation="MRFNNPIYENEELTYQDVFLFQDYFEGKSRFEVEIKPTNSLGTE
                     IPIVVANMNAVAGKRMAETIARYGGLAVLPQDMSIETLERVIKHVKSANPKYDTPITV
                     KAKNTIRDAMGIIHKRDHNCVVMVDDNFRAIGIFTPKDFADLDQFSLLGDIKKGQLIT
                     GEVGISNEDAFNIMDEKNISSLPIVDKNGILKGILTKKNTIRNSIYKPTLDKNGKLNV
                     AVAIGINGFEEKVHRLFELGVDIFVLDTAHGYQKSMIENIKKFRKLFGNGATLIAGNV
                     ITEKATRELIKAGASGVKVGIGPGAMCTTRMKTGVGRPQFTAVYKCAKEAK"
BASE COUNT         1034 a          434 c          462 g          991 t
ORIGIN      
        1 tgaggcgtca gatttacagt ctgatgcaat tgaccgctct gccatctccc ctttttatac
       61 tttatatagt gaatacattc taccatttcc cccaataata ttagccgtga aacgccccga
      121 tacatcaggg tgcaattgac agctcttcta ataaataatt ttctactatt ctcttcaaaa
      181 taaaattaac tatgaggtgt ccaggtggat cagggaacta ttgaccactt cttcgtatat
      241 tttaaaacaa caaatacagc ataatattgt tgcttataag tatgacaact atgtatatga
      301 aaataaaaag caaaatcaag tgtttttata ttattttatc attttttcga cagaatggtt
      361 gtttttaatc tcgctttggt tctatatgct tgtttaaaaa tgagaaaaaa agatggactt
      421 tcctagcttt ataagcaaaa gaaaaagtga ggcgaggttt ttatttggat ttgattgata
      481 aaaaaagtta ctttaaaatt attttatgat atcaaagtag tataattatc ttttgaatgc
      541 ttgaagatgg tttttacttc cgttaagaag gatttggagt cattgtatta tagaagggtg
      601 atttttgaac aatggcatca tattttctaa atcaacaata tctttttctt caaccaaaat
      661 accaacctgt attgcttctg cgctacttgt ttttcatttt actataagat catcgtataa
      721 ttgttgtagt tctgtatttt tgaattctcc tgctgacatt cactcagttg gattttccaa
      781 tcagtatgct tccaaaattg ttgcgattac ggattgatgg tttatctctc cttttattat
      841 attattaaat cttttgtcgc ctcgctgatc atacattact tgatatatat cttgtgccaa
      901 cttttcttct tctataataa attgcaattc agagatttgt tcaggagtta gatcaagtca
      961 tgtatccaca acaagattca tatgattaat tcatgttgct gacgaattgc gtcagattcc
     1021 ttgttgtcat caaccacgaa ctgtttgtcc tctttttgcg tttcatcatc attgtcattg
     1081 ttgttgattt gcagttattt tagagcctct ggattgagat gttaattcta gtctgtttct
     1141 attgacttgt tcaagtgatg tacatcaagc aaataaaaga actaatccca aaactgaaaa
     1201 tagtattaat gtttttttca tgacatgttt atagtatata aaaatgagta attttttgta
     1261 ttttttataa agattttttt caacagattt tcaaaacaaa aaaaatgttt tttagcttgt
     1321 gtaaaaaaca aaacaaaccc tgtctcaata taaattgcaa attaactttt tgataataat
     1381 ctacaaataa atatgcttaa aaagtaccaa cagaatctga tttgagtggt gatcacatat
     1441 ctaatacacc aaacatatat aaaactccta atactataat accgaatcaa aacaaaacta
     1501 tagcatttct cgttcatcca agatattttt cagctcgtgc atttttacct aaaagatcaa
     1561 ctagttgcgc tgaataataa atcaaaacag cccctatcaa cataaacaat gaacccaaaa
     1621 ttatttgttt tcgcattgtg tgtttttgat atagtttgta aatgatacaa aatagcgggt
     1681 aacgggactc gaacccgtac gaacagagct gaagtctgct atgctacacc aactacatca
     1741 tacccgcaca ataacaaaaa aacattggcg tgacgcactt tatacaatat atgaatgtag
     1801 aatgtaaaat caagtatttt tgacgccaaa atgcttgtct aaaaacaaca taaggactag
     1861 cttaaatttt ttgttgcaat ttgtatatat ctttcgtata ttcaatcttg ttttatttat
     1921 ataaaatacg catatattat gagattcaac aatccaattt atgaaaatga agaattaact
     1981 tatcaagatg tatttttgtt tcaagattat tttgagtgaa aatctaggtt tgaagttgaa
     2041 ataaaaccga caaactcact ttgaacagaa atccctatag ttgtagcaaa catgaatgct
     2101 gtagcaggga aaagaatggc agaaactatc gctcgctatg gtggattagc ggttttacca
     2161 caagatatgt ccatagagac attggaaaga gttataaagc atgtaaaaag tgctaatcca
     2221 aaatatgaca ctcccatcac ggttaaagcg aaaaacacaa ttagggatgc tatgtgaata
     2281 atccacaaaa gagatcataa ttgtgtagta atggtagatg ataattttag agctatttga
     2341 atttttaccc caaaagattt tgcggatttg gatcaatttt ccttgctttg agatataaaa
     2401 aaatgacaac ttattacatg agaagtgtga atttcaaacg aagatgcttt taatataatg
     2461 gatgaaaaaa atatatcatc gttgccaatt gtagacaaaa attgaatatt gaaatgaatc
     2521 ttaaccaaaa aaaataccat tagaaatagt atttacaaac caactttgga caaaaatgga
     2581 aaactgaatg tagcagtggc tataggaata aatggttttg aagaaaaggt tcataggtta
     2641 tttgaattgt gagtagatat ttttgttttg gatacagcac atgggtatca aaagtctatg
     2701 atagaaaata tcaaaaaatt tagaaagttg tttggaaact gagctacttt aattgctggg
     2761 aatgtaatta cagaaaaagc gacccgtgaa ttaattaaag catgagcaag tggtgtaaaa
     2821 gtatgaatat gacctggagc tatgtgtacc actagaatga aaactggagt atgaagacct
     2881 caatttacag cagtatacaa atgtgcaaaa gaagcaaaaa a
//
LOCUS       DAOSID010000090         2857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_30434_length_2857_cov_10.685939, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000090 DAOSID010000000
VERSION     DAOSID010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30434_length_2857_cov_10.685939"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="PK674_03425"
     CDS             complement(<1..1178)
                     /locus_tag="PK674_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15612.1"
                     /translation="MLSLYIHIPFCKTKCPYCSFQVCPSDQMKSESFHNEINNYTQSL
                     ISEIHHYSKILKDKEIKSIYFGGGTPQLLGLRNIEKIIDTIIENFNIENLGEFSIEMN
                     PYPQNEVLTFVKTLSSKYVFFPRIRFSFGIQSFDNSVLSMAGRDIMFPGLVELFRELQ
                     KIKKGNNVFNLDFIAFGYFHKSKKGNLQLRNQSALEFFQNLATTQFIDSYSIYTLELF
                     PGSLRYYQKDDLEKNKNISQKNFQSPTPAHFSLLDSSDDFVYEEFAILKDIVLDSGYK
                     RYELSNFSLGGKSSIHNRVYREMGDYVGLGTSASSFIKNPNLALKKYLGISKNSLALR
                     WTNTLKLSNYNKGDFWDKDSIQNLTQKDLLIEEFFLTLRTDVGINDLQKFSSILVKNI
                     "
     gene            1469..2074
                     /locus_tag="PK674_03430"
     CDS             1469..2074
                     /locus_tag="PK674_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15613.1"
                     /translation="MINSINVSDETNKKLTEQFEISEQADQIESTTKHHNNEIDNMKI
                     QANEKKQSEIKDLMGKLNEDSEIQDQNDILYDCEKRVNDGKNYFNALNGDQEAIQRKI
                     KELGYVNKSTWITTSQYFLGLDDLNEEDLQKEYIKYFEPQTSVYYLGGVINAVLARAF
                     DMGFFSAMNGKEGKVDEKFSKTIRITQKLVNISRKRKAAQI"
     gene            2101..2406
                     /locus_tag="PK674_03435"
     CDS             2101..2406
                     /locus_tag="PK674_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15614.1"
                     /translation="MNDVGIQAHIDENLGENKELPDLKQGFTDNFNKLRIKIGTLYLS
                     KIKKYRNFNNQDPPGKLNQIFIEFNKVKTVEKLYLLGKDFFIGMFLSDPGSYKALTT"
BASE COUNT         1115 a          298 c          402 g         1042 t
ORIGIN      
        1 tctatatttt ttactaatat ggatgaaaat ttttgtaagt catttattca aacatcagtc
       61 ctaagagtta agaaaaactc ttctatcaac aaatcttttt gtgttaggtt ttggatagaa
      121 tctttgtccc aaaaatctca tttattataa ttacttaatt taagtgtgtt tgtccaacga
      181 agtgctaatg aattttttga aattcataaa tattttttta aggctaagtt tggattttta
      241 ataaatgaag atgctgaagt tcacaaacca acataatctc acatttctcg gtatacccta
      301 ttgtgaatgc tactttttca cccaagagag aagttggata attcgtatct tttatatcaa
      361 gaatctaaaa caatatcttt tagtatagca aactcttcat aaacaaaatc atcagaagaa
      421 tcaagaagtg aaaaatgagc aggagttggt gattgaaagt ttttttgtga aatgttcttg
      481 tttttttcta agtcatcttt ttgatagtat cgcaaactac caggaaacaa ttctaaagtg
      541 tatatggaat aagaatctat aaattgtgta gttgctaaat tttgaaagaa ctctaatgca
      601 ctttggtttc gtaattgtaa atttcatttt ttacttttat ggaaataacc aaatgctata
      661 aaatctaaat tgaatacatt attccctttt tttatttttt gaagttctct aaataattct
      721 actaagccag ggaacattat atctcttcat gccatagaca atactgagtt atcaaaagac
      781 tgtatcccaa aactaaacct aattctagga aaaaaaacat attttgagct taatgttttt
      841 acaaaagtta gaacttcatt ttgtggataa gggttcattt ctatgctaaa ttctcaaaga
      901 ttttctatat taaaattttc tattatagta tctataattt tttctatatt tcttagtcat
      961 aacaattgtg gagttcatca gccaaaataa atagatttaa tttctttatc cttaagaatt
     1021 ttggaataat ggtgtatttc tgaaattaga gattgtgtgt agttgtttat ttcattatga
     1081 aaactttctg atttcatttg atcagatgga caaacttgga aactacaata aggacatttt
     1141 gttttacaaa atgggatatg gatatataaa gaaagcatag tttagtcaaa agttaaaagt
     1201 ctgataataa gagtaagttt aatctagatt aactattttt ctcttatttt caaaggaatt
     1261 agaataataa tgtttggaaa ataatttatt gtaaatctaa taaaccgtat gtttttttat
     1321 aaggtttttg tgtagatatg aactagttat aataattatt taaactactc taaactctaa
     1381 aagcaaaaaa aatcaagtgt aaaatttgca tttttttgaa ttctgattat aatttgtata
     1441 tgtaatttta tttactaaaa aaactattat gattaactca attaatgttt cagatgaaac
     1501 aaataaaaaa ttaacagagc aatttgagat ttctgaacaa gcagatcaaa tagaatctac
     1561 tacaaaacac cacaataatg agatagataa tatgaaaata caagctaatg aaaaaaaaca
     1621 aagtgaaatt aaggatttga tgtgaaaatt aaatgaagat tctgaaattc aggatcaaaa
     1681 tgatatttta tatgattgtg aaaagagggt taatgattga aaaaattatt ttaatgcttt
     1741 aaatggagat caggaagcta tccaaagaaa aataaaggag ctgggttatg ttaataagtc
     1801 cacatggatt actacatcac agtattttct tggattagat gatttgaatg aagaagatct
     1861 gcaaaaagaa tatatcaaat actttgaacc tcaaacaagt gtatattatc tatgatgagt
     1921 tataaatgct gtacttgcta gagcatttga tatgtgattt ttttcagcta tgaatggtaa
     1981 agaatgaaag gtagatgaaa aattttcaaa aacaattcgt attacacaaa aacttgtcaa
     2041 tatttctaga aaaagaaaag cagctcaaat ataatctact acagaatata caagaattag
     2101 atgaatgatg tggggataca agctcacatt gatgaaaatt tgggtgaaaa taaagaatta
     2161 cctgatttga agcaatgatt tacagataat tttaataagt taagaataaa aatttgaact
     2221 ttatatttat caaaaataaa aaaatataga aattttaata atcaagatcc tccttgaaaa
     2281 cttaatcaga tttttattga atttaataaa gtaaaaactg tggaaaaact ttatctatta
     2341 ggtaaagatt tttttatatg aatgttttta tcagatccag gaagctataa agcgttgaca
     2401 acatagaatg aatatgaaga aattaaacga aatgaaaaga ttaaataaat atttgtttga
     2461 taatactaaa gatcaaaaat taaaatttga attaagagaa atagataaat atattgaaaa
     2521 ttttagtcat atattaaaaa tgcatctttc cagaagatta ttataataaa aaatctaaat
     2581 taaattttgc ctgccttata ttttttggtt gaaatgttgt tttgtttata taaaagtttg
     2641 ttaacaacaa agtggaagtt tatttgttgt aaattatatt gattgtttta aaaagttttt
     2701 gataatgttt ttatgctata atgcacaaag tttttgtaag gcgaattaaa ttatattact
     2761 tgataatttt aaaaattaaa ctaacttatg caacttttta aaagagtttt gttatctttg
     2821 ttccctgatg aaatgatggc aaacaatgta aatcctg
//
LOCUS       DAOSID010000091         2804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_31194_length_2804_cov_10.340487, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000091 DAOSID010000000
VERSION     DAOSID010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31194_length_2804_cov_10.340487"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1255)
                     /locus_tag="PK674_03440"
     CDS             complement(161..1255)
                     /locus_tag="PK674_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15615.1"
                     /translation="MIEGSYKITQFLPIKIDDQYVAQYVSYHTKKLNESIEHNIETGI
                     FLHTHILYMTFIYIQLLRISQNKKEEFKYSRIGLASNEKDFIKANKSPFAFSKINEKT
                     VFRFFRLIDFDDGTIGDISSCVNERNDLLHATGTNIEKLDQKLNKYLKNMEKVVKKSQ
                     GFLNGLFTNFLEAKIFGNGYEITHDDLELNLYIPYQFSEYELELLVRDDYSPTSKAII
                     DDLGFEFEQGSRECTPHRLEGETSFDYSNNNGVISLGKDKQSFEIKFSKASNRTIHVL
                     YNDPKSIENIALAIGIKNISDIADCTNFDTSSRTRTPQKGEIVILKNIYGNYCAIKIL
                     DIKDRTRNDEKDEVTFEYVINDAGSTDFSI"
     gene            complement(1259..2020)
                     /locus_tag="PK674_03445"
     CDS             complement(1259..2020)
                     /locus_tag="PK674_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15616.1"
                     /translation="MKVKKEIVVQTVIIIFLFFVGRFLFTVVKNAFFNKQNYRNLGTS
                     QQDYMATEEEYNVKKYKNITEVINMGNCDIIDIEGLTSLGYKYIDIFSGEVYNPKNDY
                     KKYTNQFYVQPGFEEAYLCVVANVIPSYQDEKGKFYLGILFNDPEYQGLVNVAYNKSG
                     TLYDYTSKGADSKLTGRMYGLELKEHDKIYRLDLNGVIVGNKDGGYKIIKPIDRLNLG
                     NKKTLRIGGFISAGEKGVIKKFRIFYKGGFIDNLE"
     gene            complement(2376..>2804)
                     /locus_tag="PK674_03450"
     CDS             complement(2376..>2804)
                     /locus_tag="PK674_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HOG15617.1"
                     /translation="EFGDMLEYKYACAKCGLTTTLNKSIEEKGEDFLNVLYSKNYKDA
                     EIINLDGDLIRVQREGIKDDDYRIDAKISKNKKGELQLMVLAGSKKEEDKTQLFLDPT
                     HERLAFDQNDSHPREVFSKVVAIFKNSTSAIGELPNDLDV"
BASE COUNT          975 a          394 c          286 g         1149 t
ORIGIN      
        1 ttggtaaata aatttctgga acttttattc caatgttttc aaaaaaagtt ttcattttca
       61 aaaaattaaa aaaaataaaa gaaacaatgg ttctatcttc ttataacatt cacaaaacat
      121 atttcaagaa aatatttcta taataatata ataactaatt ttatatacta aaatctgtac
      181 ttcaagcatc attaatcaca tattcaaaag ttacttcatc tttttcatca tttctcgttc
      241 tatcttttat atctaagatt ttaattgcac aataatttca ataaatattt tttagaataa
      301 ctatttctca cttttgaggt gttctagttc tactactagt atcaaaattt gtacaatcag
      361 caatatcaga aatattctta atgccaatcg ctaaagcaat gttttcaatg ctcttagggt
      421 cgttatataa tacatggata gttctgtttg aagctttgct aaattttatt tcaaaggact
      481 gtttatcttt tcataaactt ataactcaat tattgtttga ataatcaaaa gatgtttctc
      541 attctaatct atgtggagta cattctcttg aaccttgttc aaattcgaat cataaatcat
      601 ctattattgc ttttgatgtt ggactataat catctctaac aaggagttct aattcatatt
      661 ctgaaaactg ataaggaata tataaattta attcaagatc atcatgggtt atttcatatc
      721 aatttccaaa aatcttagct tctaaaaagt tagtaaatag tcaatttaga aatcattgtg
      781 atttttttac caccttttcc atatttttta aatatttgtt tagtttttga tctaattttt
      841 ctatgttagt tcatgttgca tgcaataagt catttctttc atttacacat gatgatatat
      901 ctccaatcgt tcaatcatca aaatcaatta aacgaaaaaa tctgaaaaca gttttttcat
      961 ttatttttga aaaggcaaaa ggggacttgt ttgcttttat aaaatctttc tcattacttg
     1021 ccagtcaaat tcgagaatat ttaaattctt cttttttatt ttgagatatt cttaaaagct
     1081 gaatataaat aaatgtcata tataaaatat gagtatgaag gaaaattcaa gtttctatat
     1141 tatgttctat tgattcatta agttttttcg tatgataaga aacatattga gcaacatact
     1201 gatcatcaat ttttattgga aggaattgtg ttattttata agatcattca atcatgtatt
     1261 attctaaatt atcaataaat catcacttat aaaatattct aaattttttt attactcatt
     1321 tttctccagc agaaataaag cctcatattc tcagtgtttt tttgtttcat aaatttaatc
     1381 tatctattgg tttaataatt ttatatcatc aatctttatt tcatacaatt actcaattca
     1441 aatctaatcg ataaatttta tcatgttctt ttagctctaa tcagtacatt cttcatgtga
     1501 gctttgaatc tgctcattta gatgtataat catataatgt tcaagattta ttgtaagcaa
     1561 catttactaa tcactgatat tctggatcat taaataatat tcataaataa aattttcatt
     1621 tttcatcttg ataagatgga atcacattag caacaacaca taaatatgct tcttcgaatc
     1681 atggttgtac ataaaactga ttagtatatt ttttataatc attttttgga ttatatactt
     1741 ctcaagaaaa aatatctatg tatttatatc atagggatgt tagtccttca atatcaataa
     1801 tatcacaatt tcccatattg attacttcag ttatgttttt gtattttttt acattatatt
     1861 cttcttcggt agccatgtaa tcttgttgag atgttcctaa atttctataa ttttgcttgt
     1921 taaaaaaagc atttttaact actgtaaata aaaatcgtcc tacaaaaaat aggaatatga
     1981 ttatcactgt ttgaactacg atttcttttt ttactttcat cagcaagaca ttataggaat
     2041 aaatacatta tataagctta aaccaagcat tacaatagta aatattaaca atataatcaa
     2101 taacagtatg ttaaatgctt ttttgttttt tatagattct aatcgtttta aaatttcatc
     2161 aataaacacc aatgcaaaaa taggtaaaaa gatatatcaa attattataa taaacacttg
     2221 tgttaatttg ttgcaaaaaa acatcaaaac attaggtaat aatgctatta ccaaggagca
     2281 taacaatatt ttatttatgt gaaatacttt ttcttttgtg tccatgcttt caaactaaat
     2341 ctttaaattt tttatgcttt ggtatatatt tcttattaaa catctaaatc gtttggtaac
     2401 tctcaaattg cagaagtaga atttttaaag atagcaacaa cctttgaaaa aacttctcta
     2461 ggatgtgaat cattttgatc aaaagcaagt ctttcatgtg taggatcaag gaataattgt
     2521 gttttgtcct cctctttttt acttcaagcc aaaaccataa gttgtaattc tcatttttta
     2581 tttttagata ttttagcatc tattcggtaa tcatcatctt taattcattc tctttgtact
     2641 cttattaaat ctcagtcaag gtttattatt tcagcatctt tataattttt cgaataaaga
     2701 acattcaaaa aatcctctca cttttcttca atacttttat ttaatgtcgt tgttaaacca
     2761 catttggcac aagcatattt atattctaac atatctcaaa attc
//
LOCUS       DAOSID010000092         2794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_31340_length_2794_cov_14.926616, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000092 DAOSID010000000
VERSION     DAOSID010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31340_length_2794_cov_14.926616"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PK674_03455"
     CDS             complement(<1..833)
                     /locus_tag="PK674_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15618.1"
                     /translation="MKIVIDNHNILSIQYPEINETKEYSYNLDGFEFCDFNKGIIVYH
                     KKQFKIIHLRDLGDKMGVFMVGQKDEELENIELNLESVEHALKLFSGFDENTGHKYYF
                     LAKYSENKVDILNDNLKKLGSKVVVDKTEKGFCIDNKDTNEDEIIYDPINFLFGLRLV
                     YGDLNIKNNELKSIKIQIPLFSSFQENEELIDECVNSLTDNNIFFKKNIQKVKDGIVY
                     QLTSSDFELLEIFAKLYQSIEKGLKISKVDELETVKLQLIEFIKTNDKIPSNGKEEVL
                     K"
     gene            complement(983..2074)
                     /locus_tag="PK674_03460"
     CDS             complement(983..2074)
                     /locus_tag="PK674_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOG15619.1"
                     /translation="MIYGILAVFSVSIHKSFTLTLALGGDPTNYFYFVRHLKSIIIAI
                     VTALIVYRIPISFFQKQKNIIIITIIVFVLQILVFVPGIGIEINGARGWLDIPFLPSI
                     QPSEFFKLGYVLFIAGWLIRKKKSIDNDNQLTLSFIVINIFSLLIFIFIPDFGSALVM
                     GLTGLIMAFYTGMGRKKVIGILVIGVSGAIIFGTIASRFSTRFAYLQKRMSYFISAPN
                     DEDSKGVGWQNEQALAAIGGGGFAGKGYGKGLQKFGYLPEAQSDFVFAAFSEEVGFLG
                     AIALLGLYFYLMYYVITKLVYVKDEYSKMIAIGLLSLIIIQAFINIGVNLKILPNTGL
                     TLPFISHGGTAIMTNIISLMILYKIIKNR"
BASE COUNT          934 a          448 c          351 g         1061 t
ORIGIN      
        1 tcttttaata cttcttcttt tcagttgctt ggaattttat cgtttgtttt tatgaactcg
       61 atgagttgta gttttactgt ttcaagctcg tctactttac ttatttttag tcatttttca
      121 atactttgat ataattttgc aaatatttct agtaattcaa aatctgaact agttagttga
      181 taaactattc catctttaac tttttgaata ttttttttga aaaaaatatt attatctgtg
      241 aggctattta cgcattcatc tataagttct tcgttttctt gaaatgatga aaataatggt
      301 atttgtattt ttatagattt taactcattg tttttgatat tcaaatcacc ataaaccaac
      361 cgtaatccaa acaaaaaatt tattggatca tatattattt catcttcgtt tgtgtcttta
      421 ttgtcgatac aaaatcattt ttctgtctta tctacaacaa ctttagatca taattttttt
      481 agattatcat ttagtatatc taccttattt tcactgtatt ttgccaaaaa atagtattta
      541 tgtccggtgt tttcatcaaa tcaactaaat aattttagag catgttcaac actttctaga
      601 tttaattcaa tattttccaa ttcttcatct ttttgtcata ccataaaaac tcacatttta
      661 tctcacaaat ctctcaaatg gatgattttg aattgttttt tgtggtaaac gattattcat
      721 ttattgaaat cacaaaattc aaatcaatct aaattataag aatattcttt tgtttcattt
      781 atttctggat actgtatact tagtatgttg tgattgtcta taactatttt catgaatcgt
      841 attctaagaa gataaagctg atagtcgcta gttgtcacta gtcgatagtc attagtcgct
      901 agtctacagt cgctagttgt tagccaatga ctaatgtcta atgactagcg actatagact
      961 attgactatc gactaacagc gactatctat tttttattat tttatataag atcatcaagg
     1021 atataatatt ggtcattata gcggttccac cgtgactaat aaatggtaat gtcagtcatg
     1081 tattcggtaa tattttcaga tttaccccaa tatttataaa tgcctgtatt ataatcaatg
     1141 acaaaagtcc gatagctatc attttactgt actcatcttt tacatatacc aattttgtga
     1201 tcacataata catcaggtaa aagtataatc ataaaagggc tatagctccc aaaaatcata
     1261 cttcttcgct aaatgccgca aacacaaagt ctgattgtgc ttctggaaga tagccaaatt
     1321 tctgtaatca ttttcaatat cactttcacg caaaccctca tcatcatatt gctgccaaag
     1381 cttgttcatt ttgccatcct actcatttgc tatcctcatc atttggtgca ctaataaaat
     1441 agctcattct tttttgtaaa taagcaaatc tagtgctaaa tctagatgct atcgtcccaa
     1501 aaataatagc accacttact catattacaa gaattccaat tacttttttt cggcccattc
     1561 atgtataaaa agccattatt agtccagtta gtcccatcac caaagcactt ccaaaatctg
     1621 gtataaaaat aaatattaat aatgaaaata tatttatcac aataaacgat aatgttagtt
     1681 gattatcatt atcaatagac ttttttttcc taatcaacca tcaggcaata aataaaacat
     1741 atcataattt aaaaaattct gaaggctgaa tactcggcaa aaacggtata tccaaccatc
     1801 atcttgctca atttatttca attcatattc atggcacaaa aactaatatt tgtaatacaa
     1861 acacaataat tgtaattatt atgatatttt tttgtttttg aaaaaagctt attggtatcc
     1921 tataaacaat cagagcggtt actatggcta taataatgct ctttaaatgt cttacaaaat
     1981 aaaaataatt tgtaggatct cctcccaaag ctaatgtcaa agtaaaagat ttatgtatgc
     2041 tcacactaaa aactgctaag attcagtata tcatgagcaa aattcaaaca actaatatcg
     2101 gcatattttt tttgatttta aaatagtact gtgactctaa ttttgtggaa agttttttca
     2161 atgctaagtt ttatgtcttt gatttttttt gttagaacat taaatgcttg ctatgcttag
     2221 cggggtgaac ttcgttcggg ataaagtgat aaattgttcg cttcgctcac gataaattga
     2281 taaaacgatg taggggcggt tatcaaccgc ccgttattac agtaaaaaac attcgtgata
     2341 aagtgatgaa ttgataaaat gttaaaatga taaaacaaca caataatgtt gtagccccgc
     2401 agaattgcgg ggctgcctta ataaataata atttgttcaa ataaaaatct gtgctaaaac
     2461 acagattttt gttttatgaa aaaataaaat catttcttac attattttgc atttacttta
     2521 catactaaaa aagtatagtt cattctagta aattggtttt atacgatttt tggaaagtta
     2581 attattctga ttgactttat aatattaaga aacgaacctt attaaaaaaa tcttcccttt
     2641 gtctcgacaa atcggggttc gactcagctt cttcgcttta caagggagga attctaatta
     2701 aaaactttat aatcgcaata ctacaggcag gttttttaac tttataaact tactacttcc
     2761 actatgtttc aaatatgact tgtttgagct acaa
//
LOCUS       DAOSID010000093         2775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_31584_length_2775_cov_16.705147, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000093 DAOSID010000000
VERSION     DAOSID010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31584_length_2775_cov_16.705147"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..2511
                     /gene="infB"
                     /locus_tag="PK674_03465"
     CDS             76..2511
                     /gene="infB"
                     /locus_tag="PK674_03465"
                     /inference="COORDINATES: protein motif:HMM:NF023413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="translation initiation factor IF-2"
                     /protein_id="HOG15620.1"
                     /translation="MPRIEDVIKDLGVAKGTFSRYYEKVTGKEITAKTVIVSDANLLK
                     IKKLLEKNGKLGAKVVKKEETKPTALKSDELLGGGGFLAGLGFTRQEETPVYEDDDED
                     EIIEDLTEEEEAEIQKVIQDEFASKPSKQDLVKKEKKSSVSVTVEDIISHSGRPAPKT
                     ITYEKPRYDKKSGGDYPRGQKVKGVITTSKGKYKETENTQIEEQKVVKKPKIATTSAN
                     LVKKEEIVIDGNISVKEFSEKMGVPLNELMRVLMQNQLMLGITANLDFDTASLIAEEF
                     GVTVKKEENTQMDLESFITGDLQSLLDMDKEASHLEERAPIVTIMGHVDHGKTTLLDY
                     LRKTAFASKEAGGITQSIGASIAECNGKKITFIDTPGHELFTDLRARGAKLTNVAVIV
                     VAADDSVMPQTIESINHAKSAGVPIIIAITKIDKEGKKNIEQIKTDLSANGITPEDWG
                     GETPIIPVSGKTGAGIPELLEAILLQAEMLELKYNPDRSAIGVVLDAHKDPKQGVVTS
                     MIVMTGTLKVGDVIVAYNTYGKVRRMQDWTGKIIRVARGGEPIQILGITDLPEAGRMV
                     EVVKNEKEASTKITLIQEQEIKQGSKSAVQDFLSKLGAGDSDEKTELRLILKSDGSSS
                     LSALEQAVLGVSTPDNVIIKIVHQDVGHFNDSDLSLAQASGALLLGFNISINSVLKKK
                     AEQMKVEMKAYDVIYELTTYLEDLTMGMIKYEEIEVVTGKLEILGIFYTKGKEMVVGG
                     KVIEGIAQNKSKFRVIRGEDIIAGGEIISLHKNKDEIKKANEGEECGMKVKVSKKIEE
                     GDIIEFWEMQEVK"
BASE COUNT         1114 a          309 c          516 g          836 t
ORIGIN      
        1 ttaaatttct tcttgaaaaa gaaaacaata taattacgat gtgtttagca gattttttag
       61 tatttacaaa agtttatgcc acgtattgaa gatgtaataa aagatttatg agttgcaaag
      121 ggtacttttt ctaggtatta tgaaaaagtt actggaaaag aaataacagc aaaaactgtt
      181 atagtttctg atgcaaattt attaaaaatt aaaaagcttt tagaaaaaaa ttgaaaacta
      241 ggtgcaaagg ttgtcaaaaa agaagaaaca aagccaacag cattaaaatc agatgaatta
      301 ttgtgaggtg gtggtttttt ggcatgactt tgatttacta ggcaagaaga aactcctgtt
      361 tatgaagacg atgatgaaga tgaaatcatt gaagatttga cagaagaaga agaggcggag
      421 atacaaaaag ttatacaaga cgagtttgct tcaaaacctt ccaaacaaga tttggttaaa
      481 aaagaaaaaa aatccagtgt ttctgttaca gtagaagata taatttctca tagtggtagg
      541 cctgctccaa agaccattac ttatgaaaaa cctagatatg acaaaaaatc ttgatgagat
      601 tatcctagat gacaaaaagt aaaatgagtt attactacaa gtaaaggtaa gtataaagaa
      661 actgaaaata cacaaattga agaacaaaaa gttgttaaaa agccaaaaat agctactact
      721 tcagcaaact tggtaaaaaa agaagaaata gttattgatt gaaatatttc tgtaaaagag
      781 ttttctgaaa agatgggagt cccactaaat gaactgatga gagttttgat gcaaaatcaa
      841 ttaatgttat gaattactgc taatttggat tttgatacag catctttgat agcagaagaa
      901 ttttgagtaa cagtaaaaaa agaagaaaat acacaaatgg atttagagag ttttattacg
      961 ggagatttac agtccttatt agatatggac aaagaagctt ctcatttaga agaaagagct
     1021 cctatcgtga ctattatggg acatgtagat catggtaaaa ctactctact tgattatctt
     1081 agaaaaactg cttttgcttc caaagaagct tgatgaatta ctcaatctat ttgagcatcc
     1141 atagcagaat gtaatggtaa aaaaattact tttatagata ctccatgaca tgaattattt
     1201 actgatttga gagccagatg agcaaaactt acaaatgtag cagtgatagt tgttgctgca
     1261 gatgatagtg tgatgccaca aactatagaa tctataaatc atgcaaaatc tgctggtgtt
     1321 ccgattatta ttgctattac aaaaatagac aaagaaggta aaaagaatat agaacaaata
     1381 aaaacagatt tatctgcaaa tggtattact ccagaagatt ggggatgaga gactccaatt
     1441 attccagttt ctggtaaaac tggagcatga attccagaac ttttggaagc gatactttta
     1501 caagcagaaa tgttagaatt gaaatataat ccagatagat ctgctatatg agtagttttg
     1561 gatgcccaca aagacccaaa acaatgagtt gttacaagta tgatagtaat gacaggaact
     1621 ctaaaagttt gagatgttat tgtggcatac aatacttatg gtaaagttag aaggatgcaa
     1681 gattggactt gaaaaataat tagagttgct agatgaggag aaccaattca aatactttga
     1741 attactgatt tgccagaagc aggtaggatg gtagaggttg tgaaaaatga aaaagaagct
     1801 agtacaaaaa taacattgat tcaagaacaa gaaataaaac aaggttctaa atcagcagtt
     1861 caagatttct tatctaaatt gtgagcttga gatagtgacg aaaaaacaga attaagatta
     1921 atcttaaaat ctgatggatc tagtagtttg agtgcattgg aacaggctgt tctttgagtt
     1981 tctacaccag acaatgttat tattaaaata gtgcatcaag atgtatgaca ttttaatgat
     2041 tcagatcttt ctttagcgca agcatctgga gcattgttgt tatgatttaa tatttctata
     2101 aattctgtat taaagaaaaa agcagaacaa atgaaagtag aaatgaaagc ttatgatgtg
     2161 atatatgaat tgactacata cttagaagat ttgactatgt gaatgataaa gtacgaagag
     2221 atagaagtag ttacttgaaa attagaaata ttatgaattt tctatacaaa gtgaaaagaa
     2281 atggttgtgg gtgggaaagt aattgagtga atcgctcaaa acaaatctaa atttagagtt
     2341 attagatgag aagatattat tgcttgatga gaaataatct ctctccacaa aaataaagat
     2401 gaaattaaaa aagcaaatga atgagaagaa tgtggtatga aagtaaaagt atccaaaaaa
     2461 atagaagaat gagatataat agagttctgg gaaatgcaag aagtgaagta gtttatcaag
     2521 ttgaaagttg gagtgttttt taatataaat atttcaaaaa caatattgat atttggacat
     2581 gaaattcaaa agaagaaagt ataaaaaaac ttttattcta ggtttattct aggtatgtta
     2641 tattatataa atagatgaaa tttattttat aagttctatg tcgctaaaat acccaaaaca
     2701 gattggtcca gtatttcatt catcggattg ttccaatatt ttcattcaag catatatttt
     2761 gataggacta ttttt
//
LOCUS       DAOSID010000094         2711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_32593_length_2711_cov_14.774849, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000094 DAOSID010000000
VERSION     DAOSID010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32593_length_2711_cov_14.774849"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PK674_03470"
     CDS             <1..1137
                     /locus_tag="PK674_03470"
                     /inference="COORDINATES: protein motif:HMM:NF025035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="adenine-specific methyltransferase EcoRI family
                     protein"
                     /protein_id="HOG15621.1"
                     /translation="CFNFSAVFFMTEILQGGNANLHQAKKDKNDEFYTQLPDIENELR
                     NYKHHFKDKVVFCNCDDPYESNFFKYFALNFNHLGLKKLIAMGYVTSPIAGSQVPLFE
                     IEGYEHIENKAYKIEVNQVKDIDNNGAFDLEDIKVLIRQNKEFIKITPLKGNDEWLAG
                     DFRSNASIKALKEADIVVTNPPFSLFREYVAQLMNYQKHFLIIGNKNAITYKEIFPLI
                     KENKLWLGYSAANEFALPDGTITKKMNGLTRWYTNLDISKRHEELILYKKYNSSEYPK
                     YDNYDAIEVGKVAEIPCDYSGAMGVPITFLDKYNPEQFEIIGLGIANLGLEIGVQPYK
                     AHHREYRKNVQKRGVVNGDLYMVDKNNNPIVPYARILIKKIKHN"
     gene            1172..2269
                     /locus_tag="PK674_03475"
     CDS             1172..2269
                     /locus_tag="PK674_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOG15622.1"
                     /translation="MASYSMEIKLHEIPIREVVAGYADNAEEGVVGYGGRLNIRPPYQ
                     REFVYQGKQRDEVINTVRKKFPLNVMYWAKNPDGNFEVLDGQQRTLSICQYVNGDYSI
                     NGNYFHSLTEDQQKDILDYKLMIYICEGEESEKLDWFRIVNIAGEKLTDQELRNSVYT
                     GPRLADAKRHFSKTACPAYQIGSKYLKGSSIRQEYLETALKRISQGNIEEYMSKNQFN
                     ENASELWQHFQSVINWINVIFPTYRKEMSGLDWGTWYNEYKNHSFDSQKLEQQVKDLM
                     EDDEVEKKSGIYPYLLTGDEKYLNLRAFSDNVKRQIYEKQNGICPHCKEHFEINQMEA
                     DHITPRSQGGKTEVSNCQMLCRECNRRKSNN"
     gene            complement(2275..>2711)
                     /locus_tag="PK674_03480"
     CDS             complement(2275..>2711)
                     /locus_tag="PK674_03480"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=25
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOG15623.1"
                     /translation="TDGIAIGGVSVGESKEKVREVVEFTTPLLPNDKPRYLMGVGTPE
                     DLLHAIEHGVDMFDCVLATRLGRHGVAFSGKGNINLNNAKFKEDFNPLDDTAEGLKNY
                     SKSYIHHLLKEKEMLGGIILSLHNILYLHKILENWKRNFLED"
BASE COUNT         1025 a          379 c          441 g          866 t
ORIGIN      
        1 tgctttaatt tttcagccgt tttttttatg acagaaattt tacaaggatg aaatgcaaat
       61 cttcatcaag ccaaaaaaga taaaaatgat gaattttata cacaacttcc tgatatagaa
      121 aacgaactta gaaattacaa acatcacttc aaagataagg ttgttttctg taattgtgat
      181 gatccttatg aaagtaattt tttcaaatat tttgcattaa attttaatca tttaggatta
      241 aaaaaattga ttgctatggg atacgttact tctcctattg ctggttctca ggtaccactt
      301 tttgagattg aatgatatga acacattgaa aacaaagcat ataaaataga agtaaatcaa
      361 gtgaaagaca tagacaataa tggagccttt gatttggaag atataaaagt tctgattaga
      421 caaaacaaag aatttattaa aatcactcca ctaaaatgaa atgatgaatg gttggctgga
      481 gattttcgtt caaatgcaag catcaaagca ttaaaagagg cagatattgt agttaccaat
      541 ccaccatttt cgctttttag agaatatgtt gcacaactga tgaattatca aaaacatttt
      601 ttgattatct gaaacaaaaa tgcaattaca tacaaagaga ttttcccatt gataaaagaa
      661 aataagcttt ggcttggata tagtgcagct aatgagtttg ctttaccaga ctgaacgatt
      721 acgaaaaaaa tgaattgact aactcgttgg tatactaatt tagatatttc taaaagacac
      781 gaagaattga tcctttataa aaaatataat tcatcagaat atcccaaata tgacaactat
      841 gatgcaatag aagttggtaa agtagcagaa attccttgtg attatagtgg tgctatgtga
      901 gttcctatta cttttctcga taaatataat ccagagcagt ttgaaattat aggtttaggt
      961 attgctaact taggattaga aattggtgtt caaccataca aagcacacca tagagaatat
     1021 agaaaaaatg ttcaaaaaag aggtgttgtt aatggtgatt tatacatggt tgacaaaaat
     1081 aataatccca tagtccctta tgcaagaata ttaattaaaa aaattaagca taattaaaaa
     1141 caataatttt tatcttatta ttatataagt tatggcatct tatagtatgg aaattaaact
     1201 acacgaaata cctattcgtg aagttgttgc tggttatgca gacaatgcag aagaatgagt
     1261 tgttggctat ggtgggagat taaacattcg tccgccttac caaagggaat ttgtatatca
     1321 gtgaaaacaa agagatgaag taatcaacac tgttcgtaaa aaattcccac taaatgttat
     1381 gtattgggct aaaaatcccg attgaaattt tgaagtttta gatggacaac aaaggacact
     1441 ctctatttgt cagtatgtga atggtgatta tagtattaat ggaaactatt ttcactcttt
     1501 aaccgaagat cagcaaaaag acattttaga ttataagttg atgatttata tttgtgagtg
     1561 agaagaaagt gaaaaattgg attggtttag aattgtaaat atagcttgag aaaaattgac
     1621 cgatcaagaa ttgagaaatt cagtctatac ttgaccacga cttgcagatg ctaaaaggca
     1681 ttttagcaaa acagcttgtc cagcttatca aataggtagt aaatatctta aaggaagctc
     1741 tattcgtcag gaatatttag aaacagcatt aaaacggata tcacaaggca atatagaaga
     1801 atacatgtca aaaaatcaat ttaatgaaaa tgcgagtgag ctttggcagc atttccaatc
     1861 cgtaatcaat tggattaatg ttattttccc aacatataga aaggaaatga gcggtttaga
     1921 ttggggaact tggtacaatg aatataaaaa ccatagtttt gatagtcaaa aactagaaca
     1981 acaagtaaaa gatttaatgg aagatgatga agtagagaaa aaatcaggaa tatatcctta
     2041 tttacttaca ggtgatgaaa aatatcttaa tctgcgcgct ttctctgata acgtaaaaag
     2101 acaaatatat gaaaaacaga actgaatatg tccacactgt aaagaacatt tcgagattaa
     2161 tcaaatggaa gccgatcata ttactccacg atcacaaggt gggaaaacag aagtttcaaa
     2221 ttgtcaaatg ctatgtcgtg agtgtaatag aagaaaatca aataattagt atttctaatc
     2281 ttccaaaaaa tttcttttcc agttttctaa aattttgtgt aaatacaaaa tattatggag
     2341 agacaaaatg attcatcaca acatttcttt ttcttttaaa agatgatgta tataagattt
     2401 gctataattt ttcaatcatt cagctgtatc atctagagga ttaaaatctt ctttaaattt
     2461 tgcattattt aaatttatat ttcatttgcc agaaaatgct actccatgcc ttccaagtct
     2521 agttgccaaa acacagtcaa acatatctac tcaatgttct atagcatgta ataaatcttc
     2581 tggtgtgcct actcacatta agtatcttgg cttatcgttt ggcaataaag gagttgtgaa
     2641 ttccacaact tctctcactt tttcttttga ttctcaaaca ctcactcatc atatagctat
     2701 cccatcagtc g
//
LOCUS       DAOSID010000095         2582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_34721_length_2582_cov_13.818757, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000095 DAOSID010000000
VERSION     DAOSID010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34721_length_2582_cov_13.818757"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..1802
                     /locus_tag="PK674_03485"
     CDS             267..1802
                     /locus_tag="PK674_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15624.1"
                     /translation="MKFFFVAEDSLYKIFKTLEKIPKGKSIEISIDSQHSLFDNERWG
                     KQIKEILDKKSIKATFVTKTETTRRFFEKIGLKTKHIEKNKFMKYLRIIYLFFFDIKK
                     FHLNTFQNRKSYTFILVIGFEVLFVLGIMYLIYSLIIPGARIEINPSQQIETIIYNFR
                     YYPFEDQDYPRYSRFLSVPYYTGSLDYRYNMSMNASNVKFLQNPSVGEIKIFNKTEKQ
                     YALVPNTRFITEDGRLFQTTNRIEIPAGYQGVPGELIIRVKAMDKDDNDILMGDRGNI
                     AKGTTLYIKNLKQSLFLKEIYAVALEDFTGGTITSDGKVTQKDIDILSEKMVSYISQQ
                     KKNIVSQNFNIKDSLLLAFNDTISTTIKKIEVPHEVGENTSLLNGYVDISFSFFYIKW
                     SDLIRAFSEYIAQRPSNKTQLVSINKNSLVFFEEDGLTEKNSTFIIPTKIEITQGYDF
                     KKDVVGIVDDIKTYIVGGDKETARNYILGFPEVASVKIKITPAWYNSIPKLRSRIRVN
                     VQN"
     gene            complement(1799..2230)
                     /locus_tag="PK674_03490"
     CDS             complement(1799..2230)
                     /locus_tag="PK674_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15625.1"
                     /translation="MIETIKTSIFKKYKKDDIKGLFFSVFNEQGELLMSNGVVYTDKV
                     LEELIDLLYQGLIKKHTNIGSIVADVVLETVELKTPQEIQTISPKDFGIVLSDGSKSG
                     VLLPNTKGVNNIQEGLKLIKEKNDLGNNVQVVKFKTDRFII"
BASE COUNT          946 a          326 c          385 g          925 t
ORIGIN      
        1 tgatgtcata atcatattaa ttaataaaaa aagaaaaaaa agtttttgac aatgattgtt
       61 gtgatttatt tattttataa taggacttaa tattattttc ttgtttcaac agtatcctaa
      121 atatggtgtt atatctttgt gatgaattac ttttgatata aaaagttata tttcattttt
      181 tgcgataatg ttttgtttgc agttgtatta caagcggaat caaaaaccaa ataatatagt
      241 ttaataaata acttaaaaca taaaatatga agtttttttt tgttgctgaa gatagtttat
      301 ataagatttt taaaacctta gaaaaaattc caaagtgaaa aagtatagag atatctatag
      361 attcacaaca ttctttgttt gataatgaac gatggtgaaa gcagataaaa gaaattttgg
      421 ataaaaaaag cattaaggca acttttgtta caaaaactga aaccactaga agattctttg
      481 aaaaaatttg attaaaaaca aagcatatag aaaaaaataa atttatgaaa tatttgagaa
      541 taatttattt gttttttttt gatataaaaa agtttcatct aaatactttt caaaacagga
      601 aaagttatac tttcatattg gtgatttgat ttgaagtttt gtttgtgctt tgaataatgt
      661 atttaattta ttcattaatt attccaggtg ctagaataga aataaatccg tctcaacaga
      721 tagaaactat aatatataat tttcgttatt atccatttga ggatcaagac tatcctcgtt
      781 attctagatt tctcagtgtt ccatattata catgatcact agattatagg tataacatgt
      841 ctatgaatgc tagtaatgtg aaatttttac aaaatccatc ggtttgagaa ataaaaattt
      901 ttaataaaac agagaaacaa tatgcattag ttccaaatac taggtttatt actgaagatg
      961 gaagactatt tcaaacaaca aaccgaattg aaataccagc tggatatcaa tgagtccctt
     1021 gagagcttat tattagggta aaagcaatgg ataaagatga taatgatata ttgatgtgag
     1081 ataggtgaaa tatagctaaa tgaacaactt tgtatataaa aaacctaaaa caaagcctat
     1141 ttttaaaaga gatttatgct gtagctttgg aagattttac atgatgaact ataacaagtg
     1201 attgaaaagt aactcaaaaa gatatagata tattatctga aaagatggtt tcttatattt
     1261 ctcaacagaa aaaaaatatt gtatcacaaa actttaatat aaaggattct ttattattag
     1321 cttttaatga cactattagt actactataa aaaagattga agttccacat gaggtttgag
     1381 aaaatacctc actattaaat ggatatgtag acattagttt tagttttttt tacataaagt
     1441 ggtcagactt aattagagcg ttttccgaat atattgctca aagaccatcc aataaaacac
     1501 aattggttag tataaacaaa aactcattgg tgttttttga ggaagattga ttgactgaaa
     1561 agaacagtac ttttattatt ccaactaaaa tagaaattac tcaatgatat gactttaaaa
     1621 aagatgttgt tggaatagta gatgatatta aaacttatat agtttgatga gataaagaga
     1681 ctgctagaaa ctatatttta tgattcccag aagttgctag tgtaaagata aaaatcactc
     1741 ctgcttggta caatagtata ccaaaactaa gatctagaat aagggtaaat gttcaaaatt
     1801 aaattataaa cctatctgtt ttgaatttaa caacttgaac attatttcat aaatcatttt
     1861 tttcttttat taactttaat cattcttgta tattgtttac tcactttgta tttggtaaca
     1921 atactccaga tttgcttcaa tcacttaata cgattcaaaa atcttttgga gatatggttt
     1981 gtatttcttg tggagtttta agctctactg tttctaaaac tacatctgct actatgcttc
     2041 caatatttgt gtgttttttt atcaaacctt gatataataa gtctattagt tcttccaaaa
     2101 ctttatcagt ataaactact caattggaca tcagcaattc tcattgctca ttaaacacag
     2161 aaaaaaatag tcatttgatg tcatcttttt tatacttttt gaaaatggaa gtttttattg
     2221 tttcgatcat taacgactgt taaaagttaa aggttaggta gatgtggtgt agatccccct
     2281 tactcattac ggagaactcc gtagacaagg gggactaatg gagatttaat gccccaactt
     2341 cgtggtaaaa ttcctcccta gggggaggat gcttccccgc agtattgcgg ggtaggtggg
     2401 ggtttcaccc ccaaccacaa acccccaact accccgataa atcgggggct acagcttccc
     2461 ccaagggaag gatttcatgg tatatagttc ctcttgatag tttttgtggt ttatatagta
     2521 tatatattat aatttttaac attttactat caacttattt tatgtctaaa ctgaaattta
     2581 aa
//
LOCUS       DAOSID010000096         2557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_35170_length_2557_cov_12.341727, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000096 DAOSID010000000
VERSION     DAOSID010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35170_length_2557_cov_12.341727"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(380..901)
                     /locus_tag="PK674_03495"
     CDS             complement(380..901)
                     /locus_tag="PK674_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15626.1"
                     /translation="MFQHFPFTCEGLKSATNKLIEFIEKPIIAPIKKIQKTFSGNKKY
                     LEESNMNIQVEEMLLGVQGIQISSQPNTAFDSMVKKINNWKENNIDQLLIDQKSYSQD
                     ICNILLQDINEKYMLKEVGLSAVMLIYFLLFGFIRLTIFVMSFIGFLLFKLLYRCGVY
                     KIKKTKIEIFEIK"
     gene            1644..1865
                     /locus_tag="PK674_03500"
     CDS             1644..1865
                     /locus_tag="PK674_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15627.1"
                     /translation="MANKYINVKVKTNPNIEQSIIEISNFNSANIKETTVRNIRIGKG
                     SSCFGISNIIKKRKDQLIAIQNTSSSVQL"
BASE COUNT         1013 a          322 c          319 g          903 t
ORIGIN      
        1 ttactttatt ttgtctcatt ctcaaagagg taaaaggtac tcaaatataa acttcttctg
       61 ctcaatattt actggctatc atagcttttt cgtaagatca tcaaggggcg actatgacgg
      121 ggtttttcat tttcaataaa ttataattaa aacttcattg atgtaatagc gattatagta
      181 taatttttct atgatgattc tcaagaaaaa aacgcgatta tttctatagt aatcatccta
      241 aattatagca taataaacaa aatgggttgc tatttcttta agtatttttt taactatata
      301 tgtaaaataa tctcatttta ccgtttttcc aatcaattca tacggtttca atattttaaa
      361 gaatagagct ttaaatattt tattttattt caaaaatctc tattttggtt ttttttattt
      421 tgtaaactca gcatcgatac agtaatttga atagtaaaaa tcaaatgaaa ctcataacaa
      481 aaatagtcaa tctaataaat ccaaataata aaaaatatat tagcatcaca gctgaaagtc
      541 aaacctcttt taacatatat ttttcattta tgtcttgtaa taaaatgtta caaatatctt
      601 gagaataaga tttttgatcg attaacaatt ggtctatatt gttttctttc caattgttta
      661 tttttttaac cattgaatca aaagctgtat tgggctgaga agatatttgt attcattgga
      721 ctcacaaaag catttcttca acctgaatgt tcatattact ttcctccaaa tattttttat
      781 ttcatgaaaa tgttttttgt atttttttaa ttggtgctat tattggtttc tctatgaatt
      841 ctataagttt gttagttgca ctttttagtc actcacaagt aaatggaaag tgttgaaaca
      901 ttcatataag ggtaaaacta tatgcaatag ttatgactaa tgtgaacaaa taacctcatc
      961 tcgagaaata ggaaatactg ttgaatccaa tggagtttaa tgaataaaaa aggattatca
     1021 aagatgatgt atatatcaat aaagataaaa ttaaccaaat atttatatct cgggaaaaaa
     1081 atagaaaaag tgctgtatca attaaaacta aaattaaaac aaaaaatatt acatatttta
     1141 tttttaggtc tttataatct atagccaaaa agtgtgaaaa aatgtagaga aaaaacatag
     1201 ctataattga agcaaatata tcatgatttc aaaacaaata aaaaatagca aaaaaatatg
     1261 agatacagac caaaaagaaa aatatatatg ttctaatttt gtatttatat ttggtcaaaa
     1321 aatttatcat ttattttgga agtttgataa aaaaatcagc aaaaaatatt agtcaccaaa
     1381 aagcaaaaat catagctggc acaaatggga ttatttgttt ttgttttgtt tttgtaaaca
     1441 tattaaatat ggaatatatt aatcaaatta ctgaactcag tataatgtat attataataa
     1501 aatcaaatag atttataata gaaaaacttg tattgttgta ataaaatgtt aatcaaaaca
     1561 agatccctat agttaaagat aggtaaacat ctcattgtca aactcattct tcatttttac
     1621 caaatctaat tttaacatat tgtttggcaa ataaatatat taatgtaaaa gtaaaaacaa
     1681 accctaatat agaacaaagt attatcgaaa tttctaattt taatagtgca aatatcaaag
     1741 aaactactgt tagaaatatt cgtattggta aatgaagttc gtgtttttga atatcaaata
     1801 ttattaaaaa aaggaaggac caattgattg caatccaaaa tacaagttca agtgttcaat
     1861 tgtaagggaa aaataaataa gttaacacaa agattaaacc agatagcaac tcaactatcc
     1921 aataaaaatt agatagtttt tttcaacaac tagaacattt tcatttttga aataaaaaac
     1981 tcaatattgg tactaattct aaggatgaaa gttttttttt acaattatca cactttgagc
     2041 gaccaaaaaa aataaatttt attcattttt tatcccattt tctagaaatt agaagcccac
     2101 caaatgaacc aaaaatagtt cccaagataa aaagtaatat gattacaaga atgttcatgt
     2161 tgaaaatgtt tgaaactaaa ttgttgataa atcttttact gaaatatatg atattgttta
     2221 ctaatttcta ctgaaactaa cagtttataa attttattgt aataaaatca agaatcctta
     2281 tcatccaagt tagagagttt ataaagttgc aagtcaatgg ttatccccct taacaagggg
     2341 gactaagggg gattttagac taatgagtgg gttttaatcc tctttagctc gcttatttac
     2401 agaattcccc gtaaataagg ggattaaata aaaacattaa tttatcataa aatcccccct
     2461 ttccccccct taaaaaaggg gggctagaac tttacaaact gccttaactt tacacacttc
     2521 aagtagaatg gaatttggga tttcaaagaa agtagaa
//
LOCUS       DAOSID010000097         2541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_35439_length_2541_cov_16.610619, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000097 DAOSID010000000
VERSION     DAOSID010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35439_length_2541_cov_16.610619"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PK674_03505"
     CDS             complement(<1..1115)
                     /locus_tag="PK674_03505"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOG15628.1"
                     /translation="MTKFIKNLIKKLIGYDKLYKFIKTSFVYQGWKKINGKIANTING
                     NPSKDFFVIGITGTNGKTTVANMLHKMLNELLAPTVMISTASIKIGNETMVNDKKMTS
                     LDVYQLQSLLADAKMQGCKIAILETSSHGLDQYRFEGITFDYAILTNITREHLDYHKD
                     MDEYIKVKKKLFQYVLENKKETKYATFPADDKIGKGWFDEMAFDKKITFSIHGSSMLR
                     AEKIVEKATGTSFDIKYLGNTYPTTCQILGEYNVYNYLTALSVGVQIGLDFNKCVESL
                     SEFTGVDGRMQKIVHNDITYFVDFAHTPDALEKTLSFLHSLKGDNRLILVFGAPGNRD
                     KLKRPEMGQIGRKYSDIVIATDDDPDTENRLAIIRQL"
     gene            complement(1430..2125)
                     /locus_tag="PK674_03510"
     CDS             complement(1430..2125)
                     /locus_tag="PK674_03510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOG15629.1"
                     /translation="MQLFVTDFKQNGDKIIISNPEILEQTRKVLRMKIGDKFFVQKEN
                     KRYQLEISSWDKFSITGKILETILQTKDVSNKNTGIVVAMPNKWSKAELIVQKLSEIG
                     VDEIYFRPSERSVLKDWNNKKGERLQKISQEAVEQSRGWKLPKIEFVKNIFPVLQNKK
                     IIVFDMVDQKVIPSYDSQANIIGIVGPEGGFTQKDYENFGKNFDLISLGDTVLRMETA
                     SIVGAWLLRNSLR"
     gene            2309..>2541
                     /locus_tag="PK674_03515"
     CDS             2309..>2541
                     /locus_tag="PK674_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOG15630.1"
                     /translation="MKKVLIVIANNGFQDYEFGVPFDMLTHAGISVSIAAGKTGECIG
                     VFGSKTVADIELEKVKGENYDMIIFIGGGGAYA"
BASE COUNT          853 a          419 c          301 g          968 t
ORIGIN      
        1 attaattgcc ttataatagc taatctattt tctgtatctg gatcatcatc agtagctatc
       61 acaatatcag aatatttccg tcatatttgt cccatttcag gtcttttcaa tttatctcta
      121 ttacctggtg ctccaaaaac taatatcaac ctattatctc attttagaga atgtaaaaaa
      181 cttaatgtct tttctaatgc atcaggggta tgtgcaaaat ctacaaaata agttatatca
      241 ttgtgtacaa tcttttgcat tctaccatcc actcctgtaa attcagataa agactcaaca
      301 cacttattaa aatctaatca tatttgcact catacactca aagccgtcaa ataattataa
      361 acattatatt ctcataagat ttgacaagta gtagggtaag tatttcataa atacttgata
      421 tcaaaagatg ttcaagtagc cttttcaaca atcttttcag ctctcagcat ggatgaacca
      481 tggatagaaa atgtgatttt tttatcaaaa gccatttcat caaaccaacc tttaccaatc
      541 ttgtcatcag ctgggaatgt agcatatttt gtttcttttt tattttccaa aacatactga
      601 aatagtttct ttttaacttt gatatattca tccatatctt tgtgataatc caaatgctct
      661 cttgttatgt ttgtaagtat cgcataatca aaagttattc attcaaatct atactgatct
      721 agtccatgag atgatgtttc caatatagct attttacatc attgcatctt tgcatctgcc
      781 aacaatgatt gtagctgata aacatccaag gatgtcattt ttttatcatt taccattgtt
      841 tcatttcaga tctttataga cgctgtacta atcatcacag taggggctaa taattcattt
      901 agcatcttgt gcaacatatt tgcaacagta gtttttcagt ttgtacctgt tattcaaata
      961 acaaaaaaat cttttgatgg atttccattg atagtatttg ctatttttca attaattttt
     1021 ttccatcatt gataaacaaa actggtctta ataaatttat acaacttatc gtatcatatc
     1081 aattttttta tcaaattttt tataaacttt gtcattccta aaaattgcct aaataaattt
     1141 accttatcag tataaaaata tcattatttt ttacaacata aattcaccat ttcatttttt
     1201 aagaaagaaa ttacaataat aaaataaaat ctatttttac agatatgtcc cgtagcacca
     1261 cgaagttttc cgtagcaagc tacattggta acaaccccct ttgtattatt ttatcatttt
     1321 ttttatttta tcatttaata ttatcctacc accgggtgca gttgattagc tctgcgagca
     1381 acacccgggt ggtaggagac cactttatca tgaacgaagt tcaccccgct tagcggagcg
     1441 aatttctaag taaccaagct caaacaatac tagctgtttc catcctaaga acagtgtctc
     1501 caagtgaaat taaatcaaaa ttttttcaaa aattttcata atctttttga gtaaatcatc
     1561 actctggtca aacaattcat ataatatttg cttgtgagtc atacgaagga ataacttttt
     1621 gatccaccat atcaaaaaca ataatttttt tattttggag tactggaaaa atatttttta
     1681 caaattctat tttgggaagt ttccatcatc gtgactgctc tactgcttct tgactaattt
     1741 tttgtaatct ttctcacttt ttattgttcc aatcttttaa cacagatctt tcactaggtc
     1801 ggaaatatat ttcatctact caaatttcag aaagtttttg aacaattagt tctgcctttg
     1861 accatttatt tggcatagct acaacaattc atgtattttt atttgaaaca tcttttgtct
     1921 gtaaaatagt ttccaaaatt tttccagtaa tagaaaactt atcccaacta gagatctcta
     1981 actgatatct cttgttttct ttttgaacaa aaaatttatc tcatattttc attctcaaaa
     2041 cttttctagt ttgctccaaa atttctggat ttgatattat tattttatct caattttgct
     2101 tgaaatctgt gacaaaaagt tgcattattt acatggataa aactataaaa gaaagattta
     2161 atttattatt ccaacgaata atctttaatc gttttaaatt taaacaatcc ttttcaaaaa
     2221 tctagtaaaa tacaaaaagt ctttaaaatt tgaaattgtt tttgaaataa gtataaactt
     2281 ataaaattta tcattaaaaa tttaaattat gaaaaaggtc cttatagtaa tagcgaacaa
     2341 cggatttcaa gattatgaat tttgagtacc ttttgatatg ttaacacacg cttgaatctc
     2401 tgtaagtata gctgcttgaa aaacttgaga atgtatctga gtttttggct caaaaactgt
     2461 agcagatata gaattagaaa aagtaaaatg agaaaattat gatatgatca tattcatatg
     2521 atgatgagga gcttatgctc a
//
LOCUS       DAOSID010000098         2531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_35621_length_2531_cov_17.518578, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000098 DAOSID010000000
VERSION     DAOSID010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35621_length_2531_cov_17.518578"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PK674_03520"
     CDS             <1..608
                     /locus_tag="PK674_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15631.1"
                     /translation="CLYFLFLMKIIAVDLDEILAETARALLKRKKNRINGKRINWNEL
                     SSYNLWEIEKLEISKQKSVEIFLWFLVGAGLWNKIDPVVGAKTKLKEFKKRGYKLHVV
                     TARHFLLRFATGIRLYLNYKHIFDSVEFANFFTRFSTKKSEICKKLGADIIIEDNLEN
                     AIECANEGIKVYLIDKPWNQAYDKKKHKGIIKVNSWSEIEI"
     gene            790..1212
                     /gene="dut"
                     /locus_tag="PK674_03525"
     CDS             790..1212
                     /gene="dut"
                     /locus_tag="PK674_03525"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="dUTP diphosphatase"
                     /protein_id="HOG15632.1"
                     /translation="MEIKFKRISDDAIVPTQNTATDAGYDLFSTEDYVLKKRERKLFK
                     TNIVCAVPEGFYARIAPRSGLAYKHGIDVLAGVIDSGYRGDVGVILINFGEDDFVVNK
                     GDKIAQLIIEKCHYVKWVEVENLDETERNEGGFGSSGK"
     gene            1290..2063
                     /gene="map"
                     /locus_tag="PK674_03530"
     CDS             1290..2063
                     /gene="map"
                     /locus_tag="PK674_03530"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOG15633.1"
                     /translation="MAKGEIIIKTKKQIENIRKSGQYLNELLYLIKDNCKAGITLMDL
                     ENIAQNFIDKNNLKGAFKGYNGFPANLCLSVNDCVVHGIPDDYVLKNGDLLKVDCGII
                     YKGGVSDSAFAVVIGGEMANPLAHELTIVTKNALDLALEKVGPGKFSYDYSREVYNIV
                     KNKGFNIIKSLTGHGVGVKVHEKPNIYNRPHPESKNIKFQPGMVVCFEPITAIESSEV
                     VLKGDNNWNLYCKKGDIGSHWEYMVLITENGYEILSGIV"
     gene            complement(2060..>2531)
                     /locus_tag="PK674_03535"
     CDS             complement(2060..>2531)
                     /locus_tag="PK674_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=25
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOG15634.1"
                     /translation="MILGIDPGIKKLGYALIQPDLKIIDAGILELEQQKFDRIQQYQR
                     ILEITNFFEDLFKKHKIQKVVMEKYFITSFNLKNAEFIYGMRGSILSLVLKNNIKIEE
                     YTPIELKKRVTGNAKADKQLVQKFITKYFKLKDIPEFHDTADALGLAFIGTKTN"
BASE COUNT          935 a          276 c          449 g          871 t
ORIGIN      
        1 tttgtttata ttttttgttt ttgatgaaaa ttatagccgt agatcttgat gaaattttag
       61 ctgaaacagc acgcgctctt ttgaagcgta aaaaaaatcg tataaattga aaacgaataa
      121 attggaatga actttcaagt tataatttgt gggaaattga aaaattggaa ataagtaaac
      181 aaaaatcagt tgagattttt ttgtggtttt tagttggagc tggactttgg aacaaaatag
      241 atcctgttgt tggagcaaaa accaaattga aagagttcaa aaaaaggtga tataaattgc
      301 atgttgtaac tgctagacac tttctattga gatttgctac ttgaatacgg ctttatctaa
      361 attataaaca catttttgat agtgtggagt ttgctaattt ttttaccaga ttttctacca
      421 agaagtctga aatttgtaaa aaactatgag ccgatataat tattgaagat aatttagaaa
      481 atgctattga atgtgctaac gaatgaatta aagtttattt aatagataaa ccttggaatc
      541 aagcttatga caaaaagaaa cataaatgaa taatcaaagt aaattcttgg tctgaaatag
      601 agatatagtg agttgaaagt ttgtagtagg tggagcaaag tcccccttaa caagggggct
      661 taagggggat taaataaaaa ctctcattta tcacaaaatc cccccttccc cccttattaa
      721 ggggggtgta acttgtaact tttaacttgt aactttacaa actatttatt atctaaatta
      781 tattcaaaga tggaaataaa gtttaaaaga ataagtgatg atgcaatagt tcctactcaa
      841 aataccgcta ctgatgctgg atatgatctt ttttctactg aagattatgt tttgaaaaaa
      901 cgagaaagaa aattatttaa aacaaatatt gtttgcgctg ttccagaagg attttatgct
      961 agaatagctc ctcgtagtgg tttagcttac aaacatggaa ttgatgtttt ggcatgagta
     1021 atagatagtg gatatagagg agatgtttga gtgattttga taaactttgg agaagatgat
     1081 tttgttgtaa acaaatgaga taaaatagct caactgataa tagaaaaatg tcattatgta
     1141 aaatgggtag aagtggaaaa tttagatgag acagaaagaa atgagtgagg atttggctca
     1201 agtgggaagt aaaaaaataa aaaaataaag ctacttattg ataggcaaga gatagaaatg
     1261 tgtatttata ttaattaaat ttgtcaaata tggcgaaggg ggaaattatt ataaaaacta
     1321 aaaaacagat agaaaatatt cgtaaatctg ggcagtattt aaatgaactt ttgtatttaa
     1381 tcaaagataa ctgtaaggct gggataactt tgatggactt ggaaaatata gctcaaaatt
     1441 ttatagataa aaataattta aaatgagctt ttaaatgata taatggtttt cctgcaaatt
     1501 tatgtttgtc ggtaaatgat tgtgttgttc atggaatacc agatgattat gttttgaaaa
     1561 atggagatct tttgaaagta gattgtggaa taatatataa atgatgagta tctgatagtg
     1621 cttttgctgt agtaatatga tgagaaatgg ccaatcctct tgcccatgaa ttaactatag
     1681 taacaaaaaa tgctttggat ctagctttag aaaaagtttg accatgaaaa tttagttatg
     1741 attattcaag agaagtttat aatatcgtga aaaataagtg atttaacata attaaatctt
     1801 taactggaca tggagtggga gtaaaggtac atgaaaaacc aaatatttac aatcgaccac
     1861 atccggaaag taaaaatata aaatttcaac catgaatggt agtttgtttt gagcccatta
     1921 ctgcgataga aagtagcgag gtagttttaa aatgagataa taattggaat ttgtattgta
     1981 aaaaatgaga tataggatct cattgggaat atatggtctt aattacagaa aatggatatg
     2041 aaatactttc aggaatagtt taatttgttt ttgtgccaat aaaagctaat cccaacgcat
     2101 ctgctgtatc atgaaattct gggatatcct ttaatttgaa atattttgta ataaattttt
     2161 gtactaactg tttgtctgct tttgcatttc cagtaactct ttttttcaat tcaattggag
     2221 tatactcctc tattttgatg ttatttttta aaaccaaact aagaattgat catctcatac
     2281 cataaataaa ttcagcattt tttaagttaa acgaggtaat aaagtatttt tccattacga
     2341 ctttttgaat tttatgtttt ttgaataaat cttcaaaaaa attagtgatt tccaaaatcc
     2401 tttgatactg ttgaattcta tcaaattttt gttgctctaa ttctaaaata cctgcatcta
     2461 taattttcaa atcaggttgg ataagagcat atcaaagctt tttaattcag ggatctattc
     2521 aaagtatcat t
//
LOCUS       DAOSID010000099         2511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_35978_length_2511_cov_14.445847, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000099 DAOSID010000000
VERSION     DAOSID010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35978_length_2511_cov_14.445847"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /gene="cysS"
                     /locus_tag="PK674_03540"
     CDS             <1..1320
                     /gene="cysS"
                     /locus_tag="PK674_03540"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOG15635.1"
                     /translation="YSCGPTVYRDPSIGNYRATFTADLIRNTLKLFGYKVISVMNITD
                     VGHLTSDADDGEDKLEKGAKREGISVWEVAKKYENIFMKGMKALNIDTFDVMPRATDH
                     LAEQISLVQKLEEKAYTYEVPGDGIYMDTSKVENYGKLMGPNYKKRLEDLNAGIRVDM
                     GGKRNPTDFALWKFNVTGQKRDMERDSPRGIGFPGWHIECSAMSSKYLGEQFDIHHGG
                     ADHITVHHSNEIAQSECGFGVHPWVKYWVHNEFLQVDGGKMSKSLGNVYTLEDVQKRG
                     YSPLDLKYFYFMAQYSNFQNFTWEQLDVAKNTRQALIRKLQGREELKDRKEFENDPFF
                     KEAMEAVSDNINTPKLLAIIQSSLNNVNENTYAIITFFENNFLKLGLFEEQEEVEIPS
                     EVQELAQKRREAKKNKDWNTADSLRDQLTSLGWKILDRPDGFDLEKI"
     gene            1406..>2511
                     /locus_tag="PK674_03545"
     CDS             1406..>2511
                     /locus_tag="PK674_03545"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="CapA family protein"
                     /protein_id="HOG15636.1"
                     /translation="MIFLFSSIFVLVVFSENRKLVNYTSNFKNALNLKFTVLNPFPEK
                     ERIKSVYLIAGGDIMLSRNIGRLGKLEGYDRIFGSGNYNPINEFNGCNDENCILFFNL
                     ESLFYTPDNDIREAGFMFRANPNNVQTLSQLKGNKDLVLSLANNHTINGGFKGIKMTK
                     KILDDYGIHYIGAGFDLKESREIKTIKKNDIKVCSQAYSYDGQYIKVGEGKISWNKIN
                     EKDIIEDIHYMKFLDCDVKIIMLHWGAEYYTKPNASQRNLAKNLVQAGADLIFGGHSH
                     VPGIFTKIDDKFVFYSLGNFIFDQRWGISSNIKNANYIYDYGIKRYTIPTYISLLGGI
                     EITKSSGGVDIKLDKIVMSEVENGIHSPLNEETY"
BASE COUNT          960 a          283 c          432 g          836 t
ORIGIN      
        1 tattcgtgtt gacctactgt ttatcgagat ccaagtatat gaaattatag agcaacattt
       61 actgcagatc tgattagaaa tactctaaaa cttttttgat ataaagtaat ttctgtgatg
      121 aatatcactg atgttggaca tttgacttca gatgctgatg atggagagga taagcttgaa
      181 aagtgagcaa aaagagaatg aatatctgtt tgggaggtgg caaaaaaata tgaaaacata
      241 tttatgaaag gtatgaaagc tcttaatatt gatacttttg atgtgatgcc tagagcgaca
      301 gaccatcttg ccgaacaaat ttctcttgtt caaaaattag aagaaaaagc ttatacttat
      361 gaagtccctg gtgattgaat ttatatggat actagtaagg ttgaaaatta tggaaagctt
      421 atgtgaccaa actataaaaa gagattggaa gatctaaatg cttgaataag agtagatatg
      481 tgaggaaaaa gaaatcctac tgattttgca ttatggaaat ttaatgttac tggtcaaaaa
      541 agagatatgg agcgggattc tccacgatga atatgatttc caggttggca tatagaatgt
      601 agtgctatgt ctagtaaata ccttggtgaa caatttgata ttcatcattg atgagcagat
      661 catatcacgg ttcatcattc aaatgaaata gcacaatctg aatgcggatt tggtgtccat
      721 ccttgggtaa agtattgggt acacaatgaa ttccttcaag tagattgagg taaaatgagt
      781 aaatcattat gaaatgtata tactttggaa gatgtacaaa aaagatgata ttctccgttg
      841 gatttgaaat atttttattt tatggctcaa tattctaact ttcaaaactt tacttgggaa
      901 caattggatg ttgcaaaaaa tacgagacaa gctctaatca gaaaactaca atgaagagaa
      961 gaattaaaag atagaaaaga atttgaaaat gatccatttt tcaaagaagc tatggaagct
     1021 gtttctgata atattaatac accaaaatta ttggcaataa tccaatcgtc gttaaataat
     1081 gttaatgaaa atacatatgc aattattact ttttttgaaa ataatttttt aaaattatga
     1141 ttatttgagg aacaagaaga agtagaaatt ccatcagaag ttcaagaatt agcacaaaaa
     1201 agacgagaag caaaaaagaa taaagattgg aatacggcag attctttgag ggatcagctc
     1261 acttctcttt gatggaaaat attggatcgt ccagatggat ttgatttaga aaaaatttaa
     1321 ttaaaatata attatatttt cttttatgag taacgacaaa acaacaaaaa agcttttgat
     1381 taaaaaaata atgttaatat ttgctatgat ttttttattt tcatctattt ttgtcttagt
     1441 tgttttttct gaaaatagga aattagttaa ttacaccagt aatttcaaaa atgccttaaa
     1501 tttaaaattt actgtactaa atccgtttcc agaaaaggaa agaataaaat ctgtatattt
     1561 aatagctgga tgagatatta tgctttctag aaatatttga cgcttaggta agttggaagg
     1621 atatgatcgt attttttgat catgaaatta caatcctata aacgagttta attgatgtaa
     1681 tgatgaaaat tgtattttat ttttcaattt agaatcctta ttctatactc cagacaacga
     1741 tattagagaa gcatgattta tgtttagagc aaatcctaac aatgttcaaa cattatctca
     1801 attgaaatga aacaaagatt tggttttatc tttggcaaat aatcacacta tcaatggatg
     1861 atttaaatga ataaaaatga ccaaaaaaat tttggatgat tattgaattc attacatttg
     1921 agcatgattt gatttaaagg aaagtagaga aataaaaaca atcaaaaaaa atgatataaa
     1981 agtctgttct caagcttatt cttatgactg acagtacatt aaagtttgag aatgaaaaat
     2041 ttcatggaat aaaataaatg aaaaagatat tatagaagat attcattata tgaagtttct
     2101 agactgtgat gtaaaaatca ttatgttgca ctggtgagca gaatattata ctaaaccaaa
     2161 tgcttctcaa agaaatctgg ctaaaaattt ggttcaagca tgagctgatt tgattttttg
     2221 atgacattct catgtcccct gaatttttac aaaaatagat gataagtttg ttttttactc
     2281 attgggaaat tttatttttg atcagaggtg gggtataagt agtaacataa aaaatgctaa
     2341 ctatatttat gattatggta taaaaagata caccataccg acttatattt cattgctctg
     2401 aggtattgaa attacaaaaa gttctggtgg cgtggatatt aaattggaca aaatagttat
     2461 gagtgaagtt gaaaattgaa ttcacagtcc attaaatgaa gaaacttatc a
//
LOCUS       DAOSID010000100         2500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_36205_length_2500_cov_16.271984, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000100 DAOSID010000000
VERSION     DAOSID010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36205_length_2500_cov_16.271984"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2172
                     /locus_tag="PK674_03550"
     CDS             <1..2172
                     /locus_tag="PK674_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15637.1"
                     /translation="RVLLSLFPDEMMANNVNPASMFISFISTKDFFSVINRKIIENYK
                     LNLFDIAIDPFEFKIGFGENRGIKKKDVDLYYGYCSTANMNGYRFLNPANIGNGLALI
                     SSISLEKIFRKYPNEYFLNFLFAIYLLNFVFLARIKVFPFDDTQKDYNWFIEILFNFY
                     ELIFEQSKHKMDYAFLGKIKREVLGEINFLFLLFEHSKLFNGVFKNSDESDEYFYKWI
                     FYDELKEAGLKNMIANFIDQREKFIYKSSFSIVERKILQYVLPADILLKYLFIDADIS
                     LVVSDIVSKVYDTDILNNKLEKFRKNKGDLLDVYMYITDYRNFKKSFFKGVQKYIMDV
                     FQNEEKISGKSLGDEIDDMMSDIGDDPENLKNLKIPERIKKESKIMEKILNFYITLVG
                     GLRIARGDNFYLRLFKKDIIKDSLSIENWSENKKNTIHYYGSLLYNYGKNVFYYKNIA
                     DNVRAGKQKFFLPYKNLSKNIYSNMHMVRMFDENFLATFLQWINTKDIKLYVNNKHIL
                     EQFKWMFSKNIGEIVKKNKNEMIDHLYKDKLKNIFEKLSLNKVLKKSLNDEDFFHIKD
                     SMYSLDFWIYDRLLDEFADLELKKGYSDTNILGILSSLMDTLFGFMLYINYLKKNNHN
                     KLDELYEIYCMDVLNISKHHSEQLIQKIKKMEKNFEKILSLWIELDDNDSFFKIASTN
                     WSNFVQGKENVYKLVSGEDVLWFRGFLKNITYYNKRYIIPK"
BASE COUNT          956 a          256 c          441 g          847 t
ORIGIN      
        1 agagttttgt tatctttgtt ccctgatgaa atgatggcaa acaatgtaaa tcctgccagc
       61 atgtttattt cttttatttc tacaaaggat tttttttctg taataaatag aaaaataatc
      121 gaaaactata agttaaatct ttttgacata gcgattgatc cgtttgagtt caaaattggt
      181 tttggagaaa atagaggaat caaaaagaaa gatgtcgatt tgtattacgg ttattgtagt
      241 acagccaaca tgaatggtta taggtttcta aatcctgcga atattggtaa tggtctggct
      301 ttgataagta gtatatcttt agaaaaaatt tttagaaaat atcctaacga gtactttttg
      361 aactttttat ttgctattta tttattaaat tttgtatttt tagctagaat caaagttttc
      421 ccctttgacg atacacaaaa agattacaac tggtttatag aaattttgtt caatttttat
      481 gagcttattt ttgaacaatc aaaacacaaa atggactatg cttttttggg gaagattaag
      541 agggaagttt tgtgagagat taacttttta tttttattat ttgagcattc caaactattc
      601 aactgagttt tcaaaaactc ggatgaaagt gatgaatact tttataaatg gattttttat
      661 gacgagctga aagaagctgg tttgaaaaat atgatagcaa attttataga tcaaagagaa
      721 aaatttatct acaaatccag cttttctatc gttgaaagaa aaatattgca atatgtttta
      781 cctgctgata ttttgttaaa gtatttgttt atagatgcag acatatcttt ggtagttagc
      841 gatattgttt caaaggttta tgataccgat attttaaaca ataagttaga aaaattccgc
      901 aaaaacaagt gagatttgct agatgtttat atgtatatca ccgattatag aaatttcaaa
      961 aaatcttttt tcaagggagt tcaaaaatac attatggatg tttttcaaaa tgaagaaaaa
     1021 attagttgaa aaagtctatg agatgaaata gacgatatga tgtcagatat ttgagatgat
     1081 ccagaaaatt tgaaaaattt aaaaattccg gaaaggatca aaaaagagtc caagattatg
     1141 gaaaaaatat tgaattttta tattactttg gtttgatgac ttcggattgc aagatgagat
     1201 aatttttatt tgagattgtt caaaaaagat ataataaaag atagcctgag tattgaaaac
     1261 tggtctgaaa acaagaaaaa tacaatacat tattatggct ctttgttgta taattatggg
     1321 aaaaatgtct tttattacaa aaatattgct gataatgtaa gagctggtaa acaaaagttt
     1381 tttttgccat acaaaaactt atccaaaaac atttattcca acatgcatat ggttagaatg
     1441 tttgatgaaa atttcttggc tacttttttg caatggatca acaccaaaga cataaaactc
     1501 tatgtaaaca acaaacatat actggagcag tttaaatgga tgttttcaaa aaatatagga
     1561 gaaatagtca aaaaaaataa aaatgaaatg atagatcatc tgtacaaaga taaattaaaa
     1621 aatatttttg aaaaactaag tttgaataaa gttctcaaaa aatccttgaa tgatgaagat
     1681 ttttttcata tcaaggactc tatgtattct ttagactttt ggatttatga tagattgttg
     1741 gatgagtttg ctgatttaga gttgaaaaaa ggctactctg atacaaatat tctatgaatt
     1801 ttatcatctt tgatggatac tttatttgga tttatgcttt atataaatta tttgaaaaaa
     1861 aataatcata acaagctgga tgaattatat gaaatctact gtatggatgt attgaatatt
     1921 agcaaacatc attcggaaca actaatacaa aaaattaaaa aaatggaaaa aaactttgaa
     1981 aaaattttga gtttatggat tgaattggac gataacgata gtttctttaa aatagcttct
     2041 acaaattggt ctaattttgt acaaggaaaa gaaaatgttt ataaactagt ttcttgagaa
     2101 gatgttttgt ggttcagggg atttttgaaa aacatcacct attataataa aagatatatt
     2161 attccgaaat aataaggaga ataattcaaa agaaataaga tagaatagga attcaagggg
     2221 gaacagggat tcaaggggga acagggattc aagggggaac aggaattcga gattgagcgg
     2281 gaattcaaga ttgaacagga attcgaggaa gaatggggat ttaaggaaga acgggaattc
     2341 aaggaagaat ggaaattcga caaagagtgg gaatctgaga aaaaaaaaga acagtttata
     2401 aaaactgttc tttggaaaga gataaaaata ggtctattta ttttgttgag gattttcgtg
     2461 ttctcatcag catcagcatt gtccaagctc taagttctgg
//
LOCUS       DAOSID010000101         2485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_36486_length_2485_cov_19.951029, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000101 DAOSID010000000
VERSION     DAOSID010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36486_length_2485_cov_19.951029"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..>2485)
                     /locus_tag="PK674_03555"
     CDS             complement(2..>2485)
                     /locus_tag="PK674_03555"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG15638.1"
                     /translation="QQSYTHSYPHCWRCHAPLISKAMDSWFIKEPELKKETIKNAKNI
                     GFVPESVKKRFIDTLESAPDWNLSRKRYWGSPIPVWQNVENPEDHFSIGTLDELYYLT
                     KTGSKNLTKNIFVRHGRTDFNDQTRYDGRGDSVLTEEGQKQAQEIANQLQYLKEEKSD
                     IVFIISPLQRAWQSIRPTFLDFFGKEKTEEMEKKYFELYKKYIVHFEDGSLQKHLQGS
                     DEQNIFQLDEQVFIDWRFTEHYGKAAQSEYEPCDYNNRSDYDKPISKDGESVNESCKR
                     VKNSVQYRNNKFANQTLIYVSHNDTIAMARKAFRNFDYASKRKAFLTKNGGIQVHYRD
                     NDTQTEVDLHRPYVDNYRGMRDGKTYKRIPEVMDCWFESGSMPFGQVGYIRRKDGTTR
                     SHRPLIYPADWIMEGLDQTRGRFRVMHVLGNAMMGKNSFDNVVINGLILAEDGLKMSK
                     SLKNYPDPEYLFFRYGTDAYRLYMLSSPSVRAEPMKFSEKGVDQIFKDFTTALSNAYK
                     FFETYAKVDKFKANSTNLYFMRHGSASGFEFNDPLTDEGIEKLTTEDFATKILRLELD
                     KIYSSTGKRATQTAEIIQKIYKDRLDKDIEIIKEENLRQDNKDKIETTYQEILKKEYG
                     NNVLIVSHEVNFEPLRQSLYGEKVSLSKYEIVKLPNCQITNDLDKWILASLHELGLEV
                     EEEMNKYYLDSATKLVLGFVDKLTNWYIRRSRRRFWASGMGQDKISGYNTFFEVLSTF
                     VKIAAPFAPFISERIYLQLQEFTNKGRVDGESVHLKHFPVSSQIYIDKKLLSEIELVR
                     KIISLGLFVRSKNNIKIKQPLSKMEIKID"
BASE COUNT          725 a          446 c          347 g          967 t
ORIGIN      
        1 tttaatcaat cttaatttcc atttttgata gtggttgttt gatctttatg ttgtttttgg
       61 atctaacaaa caatcacaat gatatgattt ttctaactaa ttctatttct gataataatt
      121 ttttatcaat atatatttga gaagatactg ggaagtgctt taaatgaaca gattctccat
      181 ctactcttca tttattagta aattcttgta gttgtaaata aatcctctca cttatgaatg
      241 gtgcaaatgg agcggctatt tttacaaaag tacttaaaac ttcaaaaaag gtgttgtaac
      301 cagatatttt gtcttgtccc attccagatg cccaaaatct tcttctagat cttctgatat
      361 accagtttgt tagcttatct acaaatccaa gtaccaattt tgttgcagaa tctaaatagt
      421 atttattcat ttcttcttct acttccaaac ctagctcgtg taaactagcc aaaatccatt
      481 tatccaagtc gttagtgatt tggcagtttg gtaatttaac aatttcgtac ttacttaaag
      541 atactttttc tccataaaga ctttgtcgta aaggttcaaa atttacttcg tgagatacaa
      601 tcaaaacatt gtttcaatat tcttttttca agatttcttg gtatgttgtt tcgatcttat
      661 ctttattgtc ttgtcgtaaa ttttcttctt ttatgatttc aatatctttg tccaatctgt
      721 ctttgtaaat tttttgtatg atttcagcag tttgtgtagc tctttttcaa gtagaggagt
      781 agattttatc taattccaat ctaagtattt ttgtagcaaa atcttctgta gtaagttttt
      841 ctattcattc gtctgtcaaa ggatcattga attcaaatcc actagcacta ccatgcctca
      901 taaaatacaa attagtacta ttagccttaa atttatctac ttttgcataa gtttcaaaaa
      961 acttataagc attgctcaat gctgttgtaa agtctttgaa tatctgatct actcattttt
     1021 ctgaaaactt cattggttca gctcttactg aaggggagga tagcatataa agtctataag
     1081 catcagttcc atatctaaaa aacaagtatt caggatctgg atagtttttt agacttttgg
     1141 acatttttag accatcctca gccaagataa gtcagtttat taccacatta tcaaaagaat
     1201 tttttcacat catagcattt cctaagacat gcattactct aaatcgtcat cttgtttgat
     1261 ctagtcattc cataatccaa tctgcaggat atatcaaagg tctatgtgat ctagtagttc
     1321 catcttttct tctaatgtat cacacctgtc caaacggcat agatccagac tcaaaccaac
     1381 aatccatcac ttccggaatt cttttgtaag tttttccatc tctcattcct cgataattat
     1441 ctacataagg tctgtgtaga tcaacctcag tttgtgtatc attatctcga tagtgaactt
     1501 gaattcctca gttttttgtt aaaaaagctt ttcttttact cgcatagtca aagttacgga
     1561 aagcttttct tgccattgca attgtatcgt tgtgagatac atatatcaaa gtctgattcg
     1621 caaacttatt gtttcggtat tgaacagaat ttttaactct tttacagctt tcattcacac
     1681 tttctccatc ttttgaaatt ggtttatcat aatcagatcg gttgttgtag tcgcaaggct
     1741 catattcgct ttgagctgct tttccatagt gttctgtaaa tctccaatca ataaacactt
     1801 gttcatccaa ttggaaaata ttctgttcat cgcttccttg taaatgtttt tgcagagagc
     1861 catcctcaaa atgaactata tattttttat ataattcaaa atatttcttt tccatttcct
     1921 cagttttttc ttttcagaaa aaatctaaga aagtgggtct gatgctttgc caagctcttt
     1981 gaagcggaga aattataaaa acaatatcag acttttcctc ttttagatat tgaagctgat
     2041 ttgcaatttc ttgtgcttgt ttttgtcatt cttcagttaa tacagaatct cagcgtcagt
     2101 cgtatctagt ttgatcatta aaatccgttc ttccatgtct aacaaaaata tttttggtca
     2161 gatttttaga tccagtttta gtcagataat acaactcatc aagcgttcat atagaaaaat
     2221 gatcttcagg attttcaaca ttttgccata cagggatcgg agacccccaa tatctttttc
     2281 tgctcaagtt ccaatcaggc gcagattcta gcgtatcaat aaatctcttt ttcacagatt
     2341 ctggcacaaa tcaaatattt ttggcatttt ttattgtttc ttttttcaat tctggctctt
     2401 tgataaacca agaatccata gcttttgaaa tcagtggagc atgacatctc caacaatgcg
     2461 gatatgagtg agtataagac tgttg
//
LOCUS       DAOSID010000102         2476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_36655_length_2476_cov_12.717059, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000102 DAOSID010000000
VERSION     DAOSID010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36655_length_2476_cov_12.717059"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..382)
                     /locus_tag="PK674_03560"
     CDS             complement(2..382)
                     /locus_tag="PK674_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG15639.1"
                     /translation="MHLTIEERELIMIYLAQNKSKREIARLIGRSHTTVNNEIKNNSV
                     RGKYNPSKAKHKAYVRRLHAKKNLKKIRCNNKLEEHIHEKIQDDGKVVLTILYIIFGG
                     MVVFVHFYSNIFANILFIFILNIV"
     gene            775..>2476
                     /locus_tag="PK674_03565"
     CDS             775..>2476
                     /locus_tag="PK674_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG15640.1"
                     /translation="MSQKELIDNLTRYWKEHKIFQKSIDQRSDTLKAITYDGPPFASG
                     EPHFGHGLVGVMKDLVGRYKTMKGYKVIRDRGRDCHGLPVEKAVEKKLGLDGKKDIEE
                     KLGIEKFTQECRAYVSNVSDEWSKFVDQTGRRADMENAYMTMNLDFMESVINVFDNLY
                     SKNLVYQGFNIQWYCPSCATALSNSEVNEGYEDRQDPAITIKFPIYNKNQEQLDKYEH
                     SSDGAINVVCCVIKRDDKFLIGYHKKGQKYVFPGGKIEKGDSIEETVIREVKEETGVE
                     VKSQKTIGEFKEILKGNIWNLIYVETEIGGEPQNMEPDKTTHFQRAEIIDSENELGMA
                     IKIENTIIDDAEEIINQFYDLYTYKNNFAGQITPDKDINILAWTTTPWTLPSNSFLAV
                     GPGIKYVLVFDKSSQEYYVVAENMLSKYYKNKDEYLLINKFSGEDMKGIFYKPLFEYI
                     NQSDISDEYKEQYFQIILGDFVSTEDGTGIVHIAPSFGQEDFEVVANIFPRENAKDWL
                     FLPVDDYGEFTDEVGDYEGIVVYDANKDIVQRLKNERKLIFQQSYTHSYPHCWRCHAP
                     L"
BASE COUNT          931 a          320 c          413 g          812 t
ORIGIN      
        1 attaaactat atttagtata aatataaata aaatatttgc aaaaatattt gaataaaagt
       61 gaacaaacac caccattccc ccaaaaatga tgtataatat agtaagaact acttttcaat
      121 catcttgaat tttttcgtgt atatgctctt ctaatttgtt gttacatcta atctttttga
      181 gatttttctt ggcatgtaac ctcctaacat aagccttatg tttagcttta ctaggattat
      241 atttacctcg tacagaattg tttttaatct cattattaac tgtcgtatgt gatcttcaaa
      301 ttaatctagc aatttcacgc ttagatttat tctgtgcgag gtaaatcatt atcaactctc
      361 tttcttcaat tgttaagtgc attaatttct cttgtaagtt tataaaggta agaactatta
      421 taactataca agtggaaact ttcaaatttt aatttaggat tttatctgat ttaaggtaaa
      481 atactgttaa aatttttaca taaaaatatt taataataat ttaatatcta ttattttgcg
      541 gtttttgaaa ctacttatga tatttttcaa taaaaggatt tctctgtatg ctcacttcgt
      601 tcacttaatt gaaatgacaa gtttctcttg aaatgataat ttatgcttta tttaccaaca
      661 taaacacaac aaaaaaactc actttcaatt gaaaaactaa gaaagattaa tacacattga
      721 gttgatgagt tttttttgtt gttatttttt atattaatat tcatttcaca aaaaatgagt
      781 cagaaagaat taatcgataa tttaaccaga tattggaaag aacataaaat ttttcaaaaa
      841 tctattgatc aaagatccga tactctaaaa gctatcactt atgatgggcc tccttttgct
      901 agtggagagc cacacttttg acatggttta gtaggagtga tgaaagatct tgtatgaaga
      961 tacaaaacca tgaagtgata caaggtaatc agagatcgag gacgagattg tcatggttta
     1021 ccagtagaaa aagccgtaga aaaaaaacta tgactagatg ggaaaaaaga tattgaagaa
     1081 aaattgggaa tcgagaaatt tacccaagaa tgtagagctt atgttagtaa tgtgagcgat
     1141 gagtggagta agtttgtaga ccagacatga agacgagcag acatggaaaa cgcttacatg
     1201 accatgaatc tagattttat ggagtctgta ataaatgttt ttgataatct atattcaaaa
     1261 aatctagtat accaatgatt caacatccaa tggtactgtc cttcttgtgc tactgctttg
     1321 tccaattctg aggtaaatga atgatatgag gatagacaag atcctgctat tactataaaa
     1381 tttcctatat acaacaaaaa tcaagaacaa ttggataaat atgaacattc atcagatgga
     1441 gctataaatg tagtttgttg tgtaatcaaa agagatgata aatttttgat atgatatcac
     1501 aaaaaatgac aaaaatatgt gttccctggt gggaaaatag aaaaatgaga tagtatcgaa
     1561 gaaaccgtaa tcagagaggt taaagaagaa acttgagtag aggtgaaatc tcaaaaaacg
     1621 atctgagaat tcaaagaaat attaaaatga aatatctgga atttgattta tgtagaaact
     1681 gaaattggat gagagccaca aaacatggaa cctgacaaaa ctactcattt ccaacgagca
     1741 gaaatcatag attctgaaaa cgagttgggg atggctataa aaatagaaaa tacaataata
     1801 gatgatgcag aagaaataat aaatcagttt tatgatttgt atacctacaa aaacaatttt
     1861 gctggacaaa taacaccaga caaagacata aatattttgg cttggacgac taccccttgg
     1921 accttgcctt caaacagttt tttggctgta tgaccaggta ttaaatatgt tttggtattt
     1981 gataaatcta gccaagaata ttatgtggta gctgaaaata tgttgagcaa atattataaa
     2041 aataaggatg aatatctttt gataaataaa ttttcatgag aagatatgaa atgaattttt
     2101 tacaaaccat tatttgaata catcaatcaa agcgatattt ctgatgagta taaggaacaa
     2161 tatttccaaa taattttatg agatttcgta agtactgagg acggaactgg gattgttcat
     2221 attgctccat catttggaca agaggatttt gaagtggtag caaatatttt tcctagagaa
     2281 aatgcaaaag attggttgtt tttaccagtt gatgactatg gagaatttac agatgaggtt
     2341 ggtgattatg aatgaatagt agtgtatgat gcaaataaag atattgtcca aagattaaag
     2401 aatgagagaa agctaatatt ccaacagtct tatactcact catatccgca ttgttggaga
     2461 tgtcatgctc cactga
//
LOCUS       DAOSID010000103         2468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_36792_length_2468_cov_17.079155, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000103 DAOSID010000000
VERSION     DAOSID010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36792_length_2468_cov_17.079155"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1067
                     /locus_tag="PK674_03570"
     CDS             228..1067
                     /locus_tag="PK674_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15641.1"
                     /translation="MTNNHEENGANLDKLLLIFRRLFFVLFVLFLLWILLFKIFKINR
                     NGIENENVEVSNKDIEVNNEDIEAENEKLEVKSGLTTNRNKNGTNLGRNYSIDGNNVL
                     YKGEIIKFADAKSFKVIDKKDLEGKDAINSDNLLGIFADYDTRVILMKNIDNIIKNKE
                     NVTLELKNSSENGMYKMKNEYEQLAQFDRGDRYEMTNEQRAKFAIMFLYIKIIKKYSN
                     NQATMEKALEELKIFLENFDKKDLVKERSLRKARGDLGVDNDCLYVDGELYACFLDDL
                     FVK"
     gene            complement(1168..2370)
                     /locus_tag="PK674_03575"
     CDS             complement(1168..2370)
                     /locus_tag="PK674_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HOG15642.1"
                     /translation="MMSIEFKQDLTEIKEIDWVTDQVKTELQGLQKEAFYKKDGEKVI
                     YDLGTVKNYLSTLEGKSWQELSSKNSSAFMMAVQIALESFKDSSGNQLYDVGKVDGLY
                     KAGGSTYQAVKKFQEDCKFKSQGKAEDGIPGKNTIQALISKLEGVSVDPVIPPASADQ
                     APVNTPPKGSKLETAPTPVASVRLDASLKNNPRVPGVLKIGDYYVPVTQELIKQGTGE
                     GYVTYDEAQKILEERQKIERQKLIKNPDQITTLTDDLIQDLFVEYKGRLSLNGLTSLT
                     DKQAESLAEHQGWLLSLDGLTSLTDKQAESLAKHGGRLYLNGLTSLTDKQAESLAEHQ
                     GWLLSLDGLTSLTDKQAESLAKHGGRLYLNGLTSLTDKQAESLAKFQGSLLLPDHLKA
                     KVDAYRTS"
BASE COUNT          844 a          399 c          412 g          813 t
ORIGIN      
        1 aaatttatga tccacaatat ggagcaaggc cagtagaaag atatatccac gataccattg
       61 agccagaaat tattcaaaaa attatggaaa aataaaaaat tttacatata ccaactgaaa
      121 aatataagag aagtactaca cttctctttt ttataataaa tctattgaaa aaaatattag
      181 aatgaatata atgaatttgt aaatatttat atattatatc attgaaaatg acaaacaatc
      241 atgaagaaaa tggggcaaat ttggataaac tcttgttgat ctttaggaga ttattttttg
      301 ttttatttgt tttgttcctt ttatggatac ttttatttaa gattttcaaa attaacagga
      361 atgggataga aaatgaaaat gtggaggtaa gcaataagga tattgaggta aacaatgagg
      421 atattgaagc agaaaatgaa aaattggaag taaaatctgg tctgacaaca aacaggaata
      481 aaaactgaac gaatttatga agaaactact ctattgatgg aaacaatgtg ctgtataaag
      541 gagaaataat aaagtttgct gatgcaaaaa gtttcaaggt gatagataaa aaagatttgg
      601 aatgaaaaga tgctatcaat tctgataatt tactaggaat ttttgcagat tatgatacta
      661 gagtgatatt gatgaaaaac atagataaca taataaaaaa caaggaaaat gttactttgg
      721 agttaaaaaa ttctagtgaa aactgaatgt acaagatgaa gaacgaatat gaacaattag
      781 ctcagtttga tcgatgagac agatatgaga tgaccaacga acaaagagcg aaatttgcta
      841 taatgttttt gtacatcaaa attattaaaa aatatagcaa taatcaagct acaatggaaa
      901 aagctttgga agaattaaag atatttttgg aaaactttga taaaaaagat ttggtaaaag
      961 aaagaagctt gagaaaagct agatgagatt tgtgagtaga taatgattgt ttgtatgtag
     1021 atggagaatt gtatgcttgt ttcttggatg acttgtttgt gaagtaaaat cttacgctaa
     1081 gcttagtttt tctcagtttt ttttcatttt caagagaggg aaaagagact tcaacgcctc
     1141 tttttttcta caaataagat aaattcttta agaagtacga tacgcatcta ccttagcctt
     1201 taagtgatct ggaagaagca aactaccctg aaattttgct agcgattccg cctgtttatc
     1261 agtcaagctt gtaagaccat tgagatacaa ccttccccca tgttttgcta acgattccgc
     1321 ctgtttatca gtcaagcttg taagaccatc aagagacaac aaccatccct gatgttctgc
     1381 tagcgattcc gcctgtttat cagtcaagct tgtaagacca ttgagataca accttccccc
     1441 atgttttgct aacgattccg cctgtttatc agtcaagctt gtaagaccat caagagacaa
     1501 caaccatccc tgatgttctg ctagcgattc cgcctgttta tcagtcaagc ttgtaagacc
     1561 attaagagac aaccttccct tatactccac aaacaagtct tgaatcaaat catcagtaag
     1621 tgtagtgatt tgatcaggat tttttatcag tttttgtctt tctatctttt gtctttcttc
     1681 caatattttt tgtgcttcat cataggtcac atacccttct cctgttcatt gtttgatcaa
     1741 ctcttgagtc actggaacat agtaatctcc tatcttcagc actcctggta cacgaggatt
     1801 attttttaga cttgcatcta aacgaaccga agccactgga gttggagcag tttctagttt
     1861 tgatccttta ggaggagtgt ttactggagc ttgatcagcc gaagctggag gaataactgg
     1921 atcaactgaa actccctcta gttttgatat aagtgcttga atcgtatttt ttcatgggat
     1981 tccatcttct gctttacctt gacttttaaa cttacaatct tcttgaaact ttttcacagc
     2041 ctgataggtt gaaccacctg ctttataaag tccatctact ttccctacat cataaagctg
     2101 attccctgaa ctatctttaa aagactccaa tgcaatttgc accgccatca taaaagcaga
     2161 actattcttg gaactcagtt cttgccaact ctttccttct aaagtggaaa gatagttttt
     2221 taccgttcag agatcgtaga taactttttc tccgtctttc ttgtaaaaag cttctttttg
     2281 aagtcattga agttcagttt tcacttgatc ggtaacccaa tcaatttctt taatttcagt
     2341 taaatcctgt ttaaattcga tactcatcat attgtataag aagataaaat tttttataat
     2401 tatactcccc ccccccctgt ataggtttag taattcggta acaaaaaagt aaaattgata
     2461 aaaatttt
//
LOCUS       DAOSID010000104         2464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_36882_length_2464_cov_18.413865, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000104 DAOSID010000000
VERSION     DAOSID010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36882_length_2464_cov_18.413865"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..469)
                     /locus_tag="PK674_03580"
     CDS             complement(2..469)
                     /locus_tag="PK674_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15643.1"
                     /translation="MNEQERQQVCSVINNFLEAKGFFPYLIDLETHPMFGPLFKNLEK
                     EQKEDIEKIIDECIVEKLNSYKTKGGELFRRFFEVNEDDFWRFRDMNCDDNFAQTKQF
                     HELGRKIEAELFKYEGILTEKMLEQEKGLDKVIGSFYNIVYTYFPRLNLVESE"
     gene            complement(794..>2464)
                     /gene="recJ"
                     /locus_tag="PK674_03585"
     CDS             complement(794..>2464)
                     /gene="recJ"
                     /locus_tag="PK674_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOG15644.1"
                     /translation="KIKVKYKILYENKDLNLIERLLKIRGIDENADIFLNPKIKDYRL
                     DPMKLNDMPKAVNRIIQALKNKEKIMIFGDYDVDGITSSFILFKFFTKFLKYRNISIM
                     YPDRIEEGYGIKNSHLDEMKEKGVSLIITVDNGITNIQEVEYAKEIGLDMIITDHHKN
                     LEKLPDAIAVINPLISKDYEFKHLAGVGVAFKLLCALLDSTKTWSEKKKNNIFNYFLP
                     IVAIGTVADIVSLTGENRLLVKKGLDLINNHRDQIPESLQGFLEYLNLKEVDTFHIGF
                     IIGPRINAGGRIDSPYNSLNTLLFTGDKQLEALQKIDEINTDRKKLQEDDFKIAEEML
                     DLDQKILIAEHEDFHEGVVGIVSGKITEKYNKPSIVFKINRDKGVAVASLRGPEYFNI
                     IEMLQKHSDLLERSGGHKQAGGLTVSLDKLDEFKKSVETYCNEIINDDDLEKILYIDT
                     KIYDDEWDYELIQGLEGLAPFGEGNKEPQLLFENVNIEKIEKVGKNGNGHMKIHGVFG
                     DKKINFLFRGKGDMIKNINTNSKINVVGKIKKDDFNGGYYVNGLELIE"
BASE COUNT          735 a          451 c          275 g         1003 t
ORIGIN      
        1 attactcact ttctactaag tttagtctag ggaaatatgt gtatacaata ttgtaaaaag
       61 atcatattac cttatccaat cccttttctt gttctagcat tttttcagtt aaaattcatt
      121 catatttaaa taactctgct tctatttttc tccctaattc atgaaattgt ttagtttgtg
      181 caaaattatc atcacaattc atatctctaa atctccaaaa atcatcttca tttacttcaa
      241 aaaatctcct aaacaactct catcattttg ttttgtatga gtttaatttt tctacaatac
      301 attcatctat gattttttcg atatcttctt tttgttcttt ttcaagattt ttaaataatg
      361 gtcaaaacat aggatgtgtt tccaaatcta ttagatatgg gaaaaatcct tttgcttcca
      421 aaaagttgtt gattacacta caaacttgtt gtctttcctg ttcgttcatt cataaagttt
      481 attttataaa agttttgatt gtttccttgt tcacatttag aacctaattg gtaacaccac
      541 cacttttgca gccccgcaat tctgcggggc tacatcaatt tgttacttca acattttatc
      601 attcaccccg ctaagcgggg caagttttat cactttatcg cgaactacgg tgcggggtag
      661 agggatttaa catcctccct aacattgcag gcgcgacccc gtaggactac ggggcgcgcc
      721 tacaggttca agtgtattgt tttatcattt tatcatgaga gaagcgaata tttatcgtga
      781 gtagagcgaa ctattattca attagctcca atcaattcac ataatatcct caattaaaat
      841 catctttttt tatttttcat acaacattaa ttttactatt ggtatttata ttttttatca
      901 tatctccctt tcatcggaat aaaaagttaa tctttttatc tccaaatact caatgtattt
      961 tcatatgtcc attcccattt tttcatactt tttcaatttt ttctatattt acattttcaa
     1021 acaaaagttg tggttcttta tttcattctc caaatggagc aagaccctct aatccttgta
     1081 ttaattcata atcccactca tcatcatata tttttgtatc aatatacaat attttttcca
     1141 aatcatcatc attgattatt tcattgcaat aagtttctac actttttttg aattcatcta
     1201 atttatctaa tgaaactgtt agtcctcaag cttgtttgtg ccctccggat ctttctaaca
     1261 aatcggagtg cttttgtagc atttctatta tattaaaata ttctggtcct cttaagctag
     1321 ctacagcaac tcatttgtct ctattaattt tgaaaacaat actcggctta ttatattttt
     1381 cagtaatttt tcacgaaact ataccaacta ctcactcatg aaagtcctca tgttccgcaa
     1441 ttaaaatttt ttgatccaaa tccaacatct cttcagcgat tttaaaatca tcttcttgta
     1501 atttttttct gtcagtattt atttcatcta ttttttgtaa agcttccaac tgcttgtctc
     1561 atgtaaataa aagtgtattc aaactgttat aaggagagtc aatccttcat catgcattga
     1621 ttcttggtca aatgataaat cctatatgaa aagtatccac ttcttttaaa ttgagatatt
     1681 ctagaaatca ctgtagtgat tctggtattt ggtcacgatg attattaatt aaatctagtc
     1741 actttttcac taacaatcta ttttctcaag ttaaggaaac aatatcagca actgttcaaa
     1801 ttgctacaat tggtaaaaaa taattaaaaa tattattttt ctttttttca ctccaagttt
     1861 ttgtactatc caataatgca cacaataatt taaatgcgac accaactcct gctaaatgtt
     1921 taaattcata atcctttgag atcaatgggt ttattacagc aatagcatca ggaagttttt
     1981 ctaaattttt gtgatggtct gtgataatca tgtccaatca aatttctttg gcatattcta
     2041 cttcctgaat gtttgtaatt cagttatcta cggtaattat cagacttact catttttctt
     2101 tcatctcatc caaatgagaa tttttgattc catatcattc ttctattcta tctggataca
     2161 taatactaat atttctgtat ttcaggaatt ttgtaaaaaa cttaaacaag ataaaactgg
     2221 aggttatccc atctacatca taatctccaa atatcataat tttttcctta tttttcaagg
     2281 cctgtattat tctatttact gcttttggca tatcatttag tttcatgggg tccaaccgat
     2341 agtcctttat ttttggattt aaaaaaatat cagcattttc atctatgcct ctaattttca
     2401 ataatctttc gattaaattt agatctttat tttcatacaa tattttatac ttcaccttaa
     2461 tttt
//
LOCUS       DAOSID010000105         2452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_37111_length_2452_cov_15.601168, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000105 DAOSID010000000
VERSION     DAOSID010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37111_length_2452_cov_15.601168"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PK674_03590"
     CDS             complement(<1..420)
                     /locus_tag="PK674_03590"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF014621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006399 - tRNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOG15645.1"
                     /translation="MKLVVQNVESASVKVVNDDGSIKKEEKINKGVLIYFGVSKKITN
                     LEFNIVKNSLDKFVDKFPRMRRLKNKDGRLDATLDDINGEILVVSNFTLYASNKNGTK
                     MDFSASGEYGKAEKIYEYFVDKLKEKFVVKTGEFGAMM"
     gene            complement(609..2381)
                     /gene="mutL"
                     /locus_tag="PK674_03595"
     CDS             complement(609..2381)
                     /gene="mutL"
                     /locus_tag="PK674_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOG15646.1"
                     /translation="MAIAKLADYVINRLKAGEVVNRPSNVLKELVENSIDAGATSLEI
                     NIVDGGKNLISVQDNGEGIQLSDMDLLLERYATSKLKTDEDLLSLSSYGFRGEALASI
                     AEVSKITVLSKTAYSEIGTKLTKRGTEKIVNHQPVPFENGTVITVEDLFYNVPARLKF
                     LKSTQTEFYYCYNYFVDVALWHHDKDFIFKKNDKVVFDLKATDDLIDRINEIFKKDWK
                     NNLKTLDYSDDDLTITGVVSDPTIRFGSSENIKIYVNSRPIQDKIIYKAIMDAYNRQI
                     SPGEFPFVVLKLDIKPELIDVNVHPSKLQVKFVDSQKIYQLVYNSVYKCLSDNKITSV
                     VHDFIGSENSGNTQNYYNKTPGQNVFLGSRDFNKKSEDNIFGFQEQKIKGFPLSNNQT
                     FDFYDDSKTYSNPDIGDYQIVGQLWNTYIVLQSNDALYYIDQHALAERIAFENMKKAT
                     SLKKELLLQPVKFSVTDIPDLQEKITQLNELGFDCSLLSENVMVVYAVPNIFITYPVD
                     LEILFNYVLYLEDINFNHVLDGVFATKACKTSIKAGHKLSYEQMANLVKEGFDSIEGM
                     FVCQHGRPFFVKIEKGSVDKMFDR"
BASE COUNT          836 a          439 c          266 g          911 t
ORIGIN      
        1 catcatcgca ccaaattctc aagttttcac aacaaatttt tcttttaatt tatcaacaaa
       61 atactcataa attttctcgg ctttcccata ttctcaacta gctgaaaaat ccatttttgt
      121 tcaattttta ttactagcat aaagtgtaaa atttgatacc accaatattt ctcaattaat
      181 atcatctaaa gtggcatcaa gtcttccatc tttatttttt aatcgcctca ttctagggaa
      241 tttatctaca aacttatcta aactattttt aacaatatta aactctaaat tcgtaatttt
      301 tttagaaact caaaaatata ttaaaacacc tttgtttatt ttctcttctt tttttatact
      361 tcaatcatca ttaacaactt taactgaagc actctctaca ttttgtacta ctaacttcat
      421 tttctacaga attaatagtt aaaaagtcct gaccacctaa ccataatact gcgggaacaa
      481 gtagaggcag aattttttta tatgaccccc ctttttttaa ggggggaagg ggggattttg
      541 tgacaaatga gagtttttat ctaatccccc ttatcaaggg ggactatcac caactttata
      601 aactaaccct atctatcaaa catcttatct acactcccct tttctatttt tacaaaaaat
      661 ggtcttcaat gctgacatac aaacatacct tcaatactat caaatcattc ttttactaaa
      721 tttgccattt gttcatatga taatttatgt cacgccttta tagatgtttt acaagctttt
      781 gtagcaaaaa ccccatccaa cacatgatta aagtttatgt cttccaaata caaaacataa
      841 ttaaatagga tttctaaatc tactggataa gtaataaata tatttggtac agcatacact
      901 accatcacat tttctgacaa caaagaacaa tcaaatcaaa gttcattaag ttgagttatt
      961 ttttcctgta aatcaggaat atctgttaca ctaaatttaa ctggttgcaa caaaagttcc
     1021 ttttttaagc ttgttgcttt tttcatattt tcaaaagcta ttctttccgc taaagcatgt
     1081 tgatcaatat agtaaagagc atcatttgat tgtaacacaa tataagtatt ccaaagttgt
     1141 catactattt gataatctca gatatcaggg ttggaatagg ttttgctatc atcataaaaa
     1201 tcaaaagttt gattgtttga taaaggaaaa cctttaattt tctgttcttg aaatcaaaaa
     1261 atattatcct cacttttttt attaaaatct ctactaccca aaaacacatt ctgtcacggc
     1321 gttttattgt aataattttg agtattccct gagttctctg atcaaataaa atcatgaaca
     1381 acactagtaa ttttattatc acttagacac ttatagacag aattatagac aagttggtag
     1441 attttttgag aatcaacaaa tttaacctgc aatttagatg gatgaacatt cacatcaatc
     1501 aattctggtt taatatccaa tttcaaaaca acaaacggaa actctcaagg agaaatctgt
     1561 ctattataag catccattat ggctttataa attattttat cctgtattgg tctagagtta
     1621 acatatattt tgatgttttc tgaagatcaa aaacgaatgg taggatcgga aacaactcaa
     1681 gttattgtta aatcatcatc tgagtaatca agtgttttca aattattttt ccagtctttt
     1741 ttgaatattt catttatcct atcaatcaaa tcatcagtag cttttagatc aaaaacaact
     1801 ttatcatttt ttttgaaaat aaaatctttg tcatgatgcc ataatgctac atcaacaaaa
     1861 taattgtagc aataataaaa ttctgtttgt gtagatttca aaaactttaa tctagctgga
     1921 acattataaa acaaatcttc aacagtaatt actgtcccat tttcaaacgg aaccggttga
     1981 tgatttacaa ttttttctgt tcatcttttg gtaagtttag tacctatttc actgtatgcc
     2041 gttttggata aaacagtaat tttggaaact tctgctatac tagccaaagc ctctcaccta
     2101 aatcaataac tagataacga taacaaatct tcatctgttt tgagctttga agtagcatat
     2161 ctttctaata acaaatccat atctgaaagc tgtattcatt ctcaattatc ctgtacactt
     2221 atcaaatttt ttcctcaatc gacaatattt atttctaaac tagtcgctca agcatcaata
     2281 ctattttcta ctaactcttt taagacattg gacggtctat ttactacttc tcatgcttta
     2341 agcctattta ttacataatc tgccaattta gctatagcca tattcaatca tattctaaat
     2401 aaaacataca actatcttct agttttttta cttgaatttt caaccaagaa tt
//
LOCUS       DAOSID010000106         2448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_37179_length_2448_cov_15.209779, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000106 DAOSID010000000
VERSION     DAOSID010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37179_length_2448_cov_15.209779"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PK674_03600"
     CDS             <1..690
                     /locus_tag="PK674_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOG15647.1"
                     /translation="EDYDVQLYMKQEYDNIGKIIDLIHYLNHDKDCVGIIVQLPLPEE
                     LKKYKSQILSTISPNKDIDGMGGVLNGLSSIDLIDFVPATPKAVVKILKEYDLYNIRG
                     KIITVIGQSNLVGKPLVLEFIKQGATVFSTNSSTPVEKTKELCKMSDIIVSCTGVVHL
                     VNDEFIRDDKSQILIDVGYGHKAGKPVGDVDFESVKDKLKYITPVPGGVGPLTVACLF
                     ENIFDIQENLK"
     gene            complement(736..>2448)
                     /locus_tag="PK674_03605"
     CDS             complement(736..>2448)
                     /locus_tag="PK674_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="GxxExxY protein"
                     /protein_id="HOG15648.1"
                     /translation="EVYIGVPFTSLRMRQNKVKDFEVLKKTIQAIHNNGAKALLTMNI
                     FPRNTDIKVFESVVEKISDTNPDAIIFSDPGTFNILRKHFPNVPLHLSTQASTLNYES
                     IKFWRDLGVKRIILARELHIDEIKEIKKHVPDIELEIFVHGAMCMSYSGRCLLGDYMS
                     GRQANKGECSHACRFKYKVWLEEERRPGKLFQLDNDKDGSYILSSKDLCTINRLAEIL
                     PYVDALKIEGRSKSEFYVGAMVKAYKEARDAILEGREPNLQAKELVEKIPHRPYWDGF
                     LFNKLTNFPEGEEVGAISDDGTMADNKGIISDDRGKDESSYLNREYDKLTFDIIGIAM
                     NVHNQLGPGLLEKVYSKGLISELKKKGYDVKKEQRVEYKINDDVVGTGYVDLIINDVV
                     ALELKSTKVTKGDYYKQLRSYTKNNENIKVGFIFNFYNKKLDFKRLDKSHPGPSNGLS
                     DGISSGLSGGLSGGLSDIVHNHQTSTSLTTPGPVFCRNYFGIFKENFIEKNGKKYFEF
                     NPKEVLERGMKLNFLSSDGMGELKIIDIIKEGGQELEKATCNTPKVFILTDKELKGRE
                     ILYE"
BASE COUNT          803 a          415 c          327 g          903 t
ORIGIN      
        1 gaagactatg atgttcaatt atatatgaaa caagagtatg acaatatttg aaaaataata
       61 gatctgattc attacttaaa tcatgataaa gattgtgttg gtattattgt tcaattacct
      121 ttaccagaag aactcaaaaa gtacaaatca cagatcttat ctacaatttc tccaaataag
      181 gatattgatg ggatgggagg agtgttaaac ggcttatcat caatagattt aatagatttt
      241 gtaccagcga caccaaaagc tgtagtaaaa atactcaaag aatatgactt atacaatatc
      301 agatgaaaaa ttattacagt tatatgacaa agtaacttgg tatgaaagcc ccttgttttg
      361 gagtttatca aacaatgagc aactgtattc tctacaaaca gtagcactcc agtagaaaaa
      421 acaaaagagc tttgcaaaat gtctgacatt atagtatctt gtacatgagt ggttcatttg
      481 gttaatgatg agtttattcg tgacgataaa agtcaaatat tgatagatgt atgatatggc
      541 cataaagcat gaaaaccagt atgagatgta gattttgagt ctgtaaaaga caaactaaag
      601 tatattacac ctgttcctgg tggagtttga cctttgactg tagcttgttt atttgaaaat
      661 atttttgata tacaagaaaa cctaaaataa ttacaagctt acttaaatat gaaaataata
      721 ttcgttcagc aatttttact catataaaat ttctcgtccc ttaagctcct tatcagtcag
      781 aataaaaact tttggagtat tacaagttgc tttttctaat tcttgtcatc attctttgat
      841 tatatctata attttcaact ctcacattcc atcagaacta agaaaattta atttcattca
      901 cctttccaaa acttcttttg gattaaattc aaaatatttt tttcaatttt tttctataaa
      961 attttctttg aatataccga aataatttct acaaaatact ggcccaggag tggtaagtga
     1021 agttgatgtc tgatgattgt ggacaatgtc agacaatcag ccagacaatc aaccagacaa
     1081 tcaactagat attcaatcag ataatcagtt agatggtcaa ggatgtgatt tatctagtct
     1141 cttaaaatct aattttttat tatagaaatt aaatataaat catactttta tattttcatt
     1201 gtttttcgta taggacctaa gttgtttata ataatctcat ttggttactt tagttgattt
     1261 taattctaaa gcaacgacat cattgatgat taaatcaaca tatccagttc ctacaacatc
     1321 atcattaatt ttatactcaa ctctttgttc ttttttaaca tcatatcatt ttttctttaa
     1381 ttcacttatt agtcacttgc tataaacttt ttctagcaat ccaggtcata actgattatg
     1441 aacattcata gctattcata ttatatcaaa tgtaagttta tcgtattctc tattcaaata
     1501 actactctca tccttccccc tatcgtcaga aattatccct ttattgtcag ccattgtccc
     1561 atcgtcagaa attgccccca cctcttctcc ttcagggaaa tttgttagtt tgttgaataa
     1621 aaatccgtcc caataaggtc tatgtggaat tttttctact aattctttag cttgtaggtt
     1681 tggttctctt cattctaaaa tggcatctct agcttctttg taagctttta ccattgctca
     1741 tacataaaat tctgatttac ttcttcattc aatttttaat gcatccacat aaggcaaaat
     1801 ctccgccaat ctatttatag tacataaatc ttttgaagac aaaatatagc tcccatcctt
     1861 gtcattatcc aattgaaata gtttccctgg tctgcgttct tcttccaacc atactttgta
     1921 cttaaatcta caagcatgac tacactctca cttgtttgct tgtctacctg acatataatc
     1981 tcacaataaa catcttcatg aataagacat acacattgct caatgtacaa agatttccaa
     2041 ctctatatct gggacatgtt ttttgatttc ttttatttca tcgatatgta actctctagc
     2101 aagtattatt cttttcactc acaaatctct ccaaaatttt atagattcat agtttagggt
     2161 agaggcttgg gtactcaaat gtaatggaac atttgggaag tgttttctaa gaatattgaa
     2221 tgtcccaggg tcagaaaaaa ttatcgcatc aggatttgta tctgaaattt tttctaccac
     2281 tgattcaaat accttaatgt cagtatttct ggggaaaatg ttcatagtta gcagtgcttt
     2341 agctcaattg ttgtgaattg cttgaattgt cttttttaat acctcaaaat cttttacttt
     2401 attttgtctc attctcaaag aggtaaaagg tactcaaata taaacttc
//
LOCUS       DAOSID010000107         2423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_37692_length_2423_cov_13.604730, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000107 DAOSID010000000
VERSION     DAOSID010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37692_length_2423_cov_13.604730"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1114
                     /gene="prfA"
                     /locus_tag="PK674_03610"
     CDS             23..1114
                     /gene="prfA"
                     /locus_tag="PK674_03610"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="peptide chain release factor 1"
                     /protein_id="HOG15649.1"
                     /translation="MIEKLKSIVQEYLRLSELAIDPETLGNQQESIKINKQISDMQEK
                     YELAKEYLNYHGQLEEAKEILNAESDEDMLEIAKEQKKESEAKLEELEQQIKIALLPS
                     DPNDDKNIFLEIRPAAGGDESALFGAELLRMYLAFAQKKGRRTEIVEEQLTDIGGVKF
                     VMVKIFGDKVYSLMKFESGVHRVQRIPDTESQGRVHTSTVTVAIMPEVDEVSIHIDPK
                     DVVMDTYAGSSCGGQNANKNQTGVRLNHKPSGLIVDIGDSKSQLKNKEKAREVLRSRL
                     YQIELDKQQKEQKDIRGNQIGSGDRSEKIRTYNFPQDRITDHRIKQSWSNIPSVLAGN
                     LDEILNTIIIENQNILLTKIEGQDSTVNN"
     gene            1271..2188
                     /locus_tag="PK674_03615"
     CDS             1271..2188
                     /locus_tag="PK674_03615"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOG15650.1"
                     /translation="MKKNIRYFIIAFLFMVFSFFLLSIGNNHTFNSKNLKNQISMVKN
                     RIEYYIEEKDGTNESHINQEIEKFNSIISRNHNIIMDIPILMFHYIQDIPSNTKDQLG
                     YKLSYSPQNLEKLLIFFQTNNIETLTFWDIIKIINGTKEIPSKAVILTFDDGHKDHYT
                     NAFPVLKKYNAKAVFFIISSKPNKDPKYANWEQIKEISNAGFEIGSHSINHTNISSLS
                     HKNIIHELEQSKIEIEEKTNSPVISFCYPSGKYSKAVLGLIGDYYIFARTTNHGKQIK
                     INERFKLPTIRINPTTKADDLKYLWEKNN"
BASE COUNT         1020 a          355 c          342 g          706 t
ORIGIN      
        1 tttattttca actccctaat aaatgattga gaaattaaaa tctatagtac aagaatactt
       61 aagactaagt gaacttgcca tagacccaga aactcttgga aatcagcaag aatctataaa
      121 aataaacaaa caaatttctg atatgcaaga aaaatatgag cttgcaaaag aatatctaaa
      181 ttaccatggt caactagaag aagctaaaga aatattaaat gctgaaagcg atgaggatat
      241 gctagaaata gcaaaagaac aaaaaaaaga atctgaagcc aaattagaag aattggaaca
      301 acaaatcaaa atagcattat tacctagtga tccaaatgat gataaaaaca ttttcttaga
      361 aatcagacct gcagcatgat gagacgaatc tgctttattt tgagcagaac tattgagaat
      421 gtatttagct tttgctcaaa aaaagtgacg aagaactgaa atagttgaag agcaactcac
      481 agatattggg tgagtaaaat tcgttatggt aaaaatattt tgagacaaag tatactcttt
      541 gatgaaattt gaaagctgag tgcatagagt acaaagaatc ccagacacag aatcccaatg
      601 aagagttcat acttctacag taaccgttgc aattatgcct gaagtagatg aggttagcat
      661 acatattgat cctaaagatg tggtaatgga tacttatgct tgatcttctt gcggaggtca
      721 aaatgctaac aaaaaccaaa catgagtgag attaaatcac aaaccaagtt gattgattgt
      781 agatatatga gatagtaaat cccaattaaa aaataaagaa aaagcacgag aggtacttag
      841 atctagattg tatcaaatag agttagataa acagcaaaaa gaacaaaaag atattagatg
      901 aaatcaaatt ggtagctgag atagatcaga aaaaataagg acatataatt ttccacaaga
      961 cagaattaca gaccatagaa tcaaacaatc ttggtcaaat attccttcag tattagcagg
     1021 gaatctagat gaaattttga atacaataat aatagaaaat caaaatattc ttttgacaaa
     1081 aatcgagtga caagattcta cagttaataa ttaataatag aaaattaaac cataaataga
     1141 gagaagccag tcatttcaat tttgaacata attagtgtaa tttagaataa aatctagaaa
     1201 tctattcgcg acacgcaaca acagcttttt attttattga aataaatttt tatgattagt
     1261 acgaaataaa atgaaaaaaa atatacgata ttttataata gcctttttgt tcatggtgtt
     1321 ttctttcttt ttattgtcta tatgaaacaa tcacactttt aacagtaaaa acttaaaaaa
     1381 tcaaatatct atggtcaaaa acagaattga atactacata gaagaaaaag acgggacaaa
     1441 tgagtcacat ataaatcaag aaatagaaaa attcaattct attatttcaa gaaatcataa
     1501 tattattatg gatatcccga ttttaatgtt tcattatatt caagatatac catctaacac
     1561 aaaagaccaa ctatgataca agttgtctta ttctccacaa aatttagaaa agttattaat
     1621 atttttccaa acaaacaaca ttgagaccct aactttttgg gacataatta aaattataaa
     1681 tgggacaaaa gaaataccat ccaaagctgt aattttaact tttgatgatg gacacaaaga
     1741 tcattataca aacgcatttc cagtattaaa aaaatataat gcaaaagctg tatttttcat
     1801 aatatccagt aaaccaaaca aagatcccaa atatgcgaac tgggaacaaa taaaagaaat
     1861 ttctaatgct ggatttgaaa tatgatcaca ttcaataaat cacacaaaca tttcatcatt
     1921 atcccataaa aatataattc acgaattaga gcaatctaaa atagaaattg aagaaaaaac
     1981 aaactctcca gtaattagtt tttgctatcc ttctgggaaa tactcaaagg cagttttagg
     2041 tttgattgga gattattata tttttgctcg tacaactaat catggtaaac aaattaaaat
     2101 aaatgaaaga tttaaactac ccacgatacg cataaatccc acaacaaaag cagatgattt
     2161 gaaatattta tgggaaaaaa ataattagct accaatcttt ccatattagc tagccatttt
     2221 taaaaaatat gcccataagt tttaacatcc aaaaatcaac agcataaaaa aggaacgatt
     2281 acaaaaccgt tccttatatt attttattta ttactttatt attttatcat ttattatatc
     2341 ctaccacccg ccccgtagga ctacggggcc gggtggttga gatgaatttc tactcctcat
     2401 ccattgattt aacttttcca gat
//
LOCUS       DAOSID010000108         2386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_38442_length_2386_cov_16.035607, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000108 DAOSID010000000
VERSION     DAOSID010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38442_length_2386_cov_16.035607"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2282)
                     /gene="pheT"
                     /locus_tag="PK674_03620"
     CDS             complement(<1..2282)
                     /gene="pheT"
                     /locus_tag="PK674_03620"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOG15651.1"
                     /translation="MKYSLNLLKKFISINDSIENLADKLTLKTVEVEEVFQRNIDQKI
                     VIGKINAVKKHPDADKLNICIVDCGSKGEYQIICGGTNVAEGIYVPTALSGAYFPKLD
                     ITIEPRKMRGEESNGMICSKAELGIDEDLDTHWIWDMQKDLDDLSDEDLGKPVVERYP
                     WLESEIIDIDNKGLTNRPDLTGHFGMSVELNSMYENEKISFNKINEYFETFKNTNIFE
                     ILDNKEKTKIGLKSETDNLNTYILMELNNISVKKSSFFTRIQMMDLGSHPISNWVDFS
                     NLFMNISGQPVHFFDAEKVVGNVIIRQAKKGEKFVDLFEKEHELLETDMVIADDQKIL
                     ALAGVVGGLDSGVTENTKNILVEIANFDPVCVRKTGVRLGLRTDAELRYEKNINPTFS
                     LFVLILFLDLLKFYKKDLGDYDLGGLSYYSKTKNANYTQNTIAIDYSKMENFIFGENK
                     GGFENDAKNILQGLGFTVRDNEIGVPFWRSPDDINIVEDIYEELARIYGYEKIDSKNL
                     YSDISLSKTSSLINLINNIEEIFVKKYNFNQVESYPWVGEKILNLFDVDLGNLYTIKN
                     PIDLDKPYLRDDLDYILINYVSKNSKFFEDFKIFDIGRVWSKLWSDGKVGKFADELVG
                     EKQQLSAMYYQKSIKNREEDLLLSAKSELEDLLKSLELKDEIIYKKTGKKAYHPNKQG
                     NIVYNGELIGFVGTIHPVILKENKLSETSDLVYLSLDLEKIDHLMSKIEFVAEGYETL
                     QDQIVWRDLCFVIDQEKDFG"
BASE COUNT          799 a          405 c          235 g          947 t
ORIGIN      
        1 tctcaaaaat ccttttcttg atctataaca aaacaaagat ctctccaaac aatttgatct
       61 tgtaaagttt catatccttc tgctacaaat tctattttac tcattaaatg atcaattttt
      121 tctaaatcca aagataaata aaccaaatca gatgtttcag ataatttgtt ttcttttaaa
      181 ataactgggt gtattgtccc aacaaaccct attagttctc aattgtaaac tatatttcat
      241 tgtttatttg gatgatatgc tttttttcca gtttttttat aaattatttc atcttttagc
      301 tccaaagact taagcaaatc ttcaagttct gatttagctg ataataataa atcttcttcc
      361 cgatttttga tacttttttg gtaatacata gctgatagct gttgtttttc tccaaccaat
      421 tcatcagcaa attttcaaac tttaccatca ctccataatt ttgaccagac tctaccaata
      481 tcaaatattt tgaagtcttc aaaaaattta ctatttttac tcacataatt tatcaaaata
      541 taatccaagt catctctcaa ataaggtttg tctaagtcta ttgggttttt gatagtgtat
      601 aagtttccca aatctacatc aaataaattt agaatcttct ctcataccca aggataactt
      661 tcaacttgat taaaattata tttttttaca aaaatttcct caatattgtt gattaagtta
      721 attagacttg atgttttact taaactaatg tcagagtata agtttttaga atctattttc
      781 tcatatcagt atatcctagc aagctcctca taaatatctt ctacaatatt tatatcatct
      841 ggtgacctcc aaaaaggaac tcaaatttca ttgtctctta ctgtaaatca aagtcattgt
      901 aaaatatttt tagcatcatt ttcaaatcat cctttatttt ctccaaaaat aaagttttcc
      961 attttagagt aatctatagc aattgtattt tgagtgtaat tagcattttt tgttttacta
     1021 taatatgaaa gtcatcataa atcgtaatct cataaatctt ttttgtaaaa tttcaacaaa
     1081 tctaaaaata atattaaaac aaaaagacta aaagtaggat ttatattttt ttcgtatcta
     1141 agttctgcat cagttctaag tcacaatctt actcaagttt ttctcacaca aacaggatca
     1201 aaatttgcta tttctactaa aatatttttt gtattttcag tcactccaga atccagtcat
     1261 caaaccacac ccgccaatgc caatattttt tgatcatcag ctattaccat atcagtttct
     1321 agtaactcat gctctttttc aaacagatcc acaaactttt ctcatttttt tgcttgccta
     1381 attatcacat ttcatacaac tttttcagca tcaaaaaaat gaacaggctg accagaaata
     1441 ttcataaata aattactaaa atccacccaa ttggaaattg gatgacttca taaatccatc
     1501 atttgtattc tagtaaaaaa actagatttt ttgacagaaa tattgttcaa ttccattaaa
     1561 atatatgtat tcaaattatc tgtttcagat tttaggccaa ttttagtttt ttctttgttg
     1621 tctaaaattt caaatatgtt ggtgtttttg aatgtttcaa aatattcatt aattttattg
     1681 aaagaaatct tttcattttc atacatggaa tttaattcta cagacatacc aaaatgacca
     1741 gtaagatcag gtctatttgt aagtcatttg ttatctatat ctattatttc agattctaac
     1801 caaggatacc tttctactac aggttttcat aaatcttcat cagacaaatc atctaaatct
     1861 ttttgcatat cccaaatcca atgagtatcc aaatcttcat ctattcacaa ttctgcttta
     1921 ctacaaatca taccgtttga ttcttctcac ctcatttttc taggttctat agtgatatct
     1981 aattttggaa aatatgcacc tgatagagca gtagggacat aaattcattc tgcaacattt
     2041 gttcctccac aaattatttg gtattctcat ttgcttcaac aatccactat acagatattc
     2101 aatttatctg catctggatg cttttttaca gcgtttattt ttcatattac aattttttga
     2161 tctatgtttc tttggaatac ttcttctact tctactgttt ttagggtgag tttgtctgcg
     2221 agattttcta tgctatcatt gatagatatg aattttttta atagattaag tgaatacttc
     2281 ataatttttt atttatacta taaaacatat attatcatat ctaataatcg caaatcaaaa
     2341 ataaagagaa aaacaaaaaa acatctatca aaatttgcaa aatttg
//
LOCUS       DAOSID010000109         2373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_38702_length_2373_cov_15.170406, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000109 DAOSID010000000
VERSION     DAOSID010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38702_length_2373_cov_15.170406"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PK674_03625"
     CDS             complement(<1..265)
                     /locus_tag="PK674_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG15652.1"
                     /translation="MSQKKFYITTAIAYPNGKPHMGHALEIIIADTLARMHRMSGKEV
                     EFQTGTDEHGIKNRRTAQKEGKDILEFLDENVSAFQDMYKKLKV"
     gene            526..1788
                     /locus_tag="PK674_03630"
     CDS             526..1788
                     /locus_tag="PK674_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15653.1"
                     /translation="MKKIAIFCVLIVVLFIISGCDLFGSDPCKGDPDSKHCYQAEAVN
                     AGDPSICAKIDGKEFKQYGSNPPKDKCYMMVAAELGNYDICNKIKGGMMSYTKEDCIM
                     EVINKNDDPGGCDHIKDKGSKQTCMENFQSANALYMKDQQIDDITQRLKDNPDDAELQ
                     AELEKVLESKKKLFPYLSSEEQSEYVKSYRENLMDDISDDDVKLAIAKEYNAYKKDNP
                     DVDVISLLDKMKKITDKQQLIKRIDEDANYLVDAVKGQMMDIAEQGKDMAVDFVKDKA
                     VDWLKTNGGDNLKWGIERLEWMKDKYDTASEEYEAINKKYQQLKETYDKIVAVYDKMD
                     SFDTLVAQGKITADKAKVLKGAVLLHNGLTAVTEYVPVFGSTVSKVTDATFETVIEVA
                     EERAARTTALDKCIDDPLNCDLDNITAY"
BASE COUNT          775 a          262 c          466 g          870 t
ORIGIN      
        1 aaacttttag ttttttgtac atatcttgga atgcagatac attttcatct aaaaattcta
       61 aaatatcttt tcattctttt tgagcagttc ttcggttttt gattccgtgt tcatctgttc
      121 ctgtttgaaa ttccacttct tttcagctca ttctgtgcat cctggctaaa gtatcagcaa
      181 taattatttc taaagcatgt cccatatgtg gttttcaatt tggataagca attgcagtag
      241 taatgtagaa ttttttttgt gacataaata ttttaaatat aaattaaaat gtattgtttt
      301 tttatgaaaa aattgttttt tttcaagttg atgtgctaaa aattttactt tgaagcttag
      361 aacttgaaat atgatagggg aaattttaaa taaattgctt gcataatgta attatatttg
      421 tagaaaatag aagctaaaaa ttttttaatt gattttttta tattttttag tagtatcagc
      481 gaaaattatc gtcttggtaa tgattttatc ctttatctta ttattatgaa aaaaattgct
      541 attttctgtg ttttgattgt tgttttgttt attatttcgt gatgtgattt atttggaagt
      601 gatccttgta aatgagatcc agatagtaag cactgttatc aggcagaggc tgtaaatgca
      661 tgagatccgt ccatctgtgc aaagatagat gggaaagaat ttaaacaata tggttctaat
      721 ccgcccaaag ataaatgtta tatgatggta gcggcagagt tgtgaaatta tgatatttgt
      781 aataaaataa agtgatgaat gatgtcttat acaaaggagg attgtataat ggaagttatc
      841 aataaaaacg atgatcctgg gtgatgtgat catattaaag ataaaggatc aaagcaaact
      901 tgtatggaaa atttccaatc tgcaaatgca ttatatatga aagatcagca gatagatgat
      961 ataacacaaa gattaaaaga taatccggat gatgctgagt tgcaagcaga attggaaaag
     1021 gtgcttgagt caaaaaagaa attattccct tatttatcat ctgaagaaca atcagaatat
     1081 gttaaaagct atagagagaa tttgatggat gatatatctg atgatgatgt aaaattggct
     1141 atagcaaaag aatataatgc ttataaaaag gacaatcctg atgtggatgt tatttcactt
     1201 ttggataaaa tgaaaaaaat aacagataaa caacagctta taaaacgcat tgatgaggat
     1261 gctaattatt tggtagatgc tgtaaaatga caaatgatgg atatagcaga gcagtgaaaa
     1321 gatatggctg ttgattttgt aaaggataaa gcagtagatt ggttgaaaac taatgggtga
     1381 gataatttaa aatgggggat tgagagattg gagtggatga aagataaata tgatactgct
     1441 tctgaggaat atgaggcaat taacaaaaaa tatcaacaac taaaggaaac ttatgataaa
     1501 atagttgctg tatacgataa aatggatagt tttgatactt tagtagctca atgaaaaata
     1561 acagcagata aagcaaaagt attgaagtga gctgtattgt tgcataatgg tttaacagct
     1621 gttactgaat atgtgcctgt gtttggaagt acggtttcta aggtaactga tgctactttt
     1681 gaaacagtta ttgaagtagc agaagaaaga gcagcaagaa caacagcatt agataagtgt
     1741 atagatgatc ctttaaactg tgatttggat aatattacgg cttattaaaa aatttatggc
     1801 ttgttttttg aagcttatct tatgtttttt tggtctaaaa atttgttttt gtgaaatttt
     1861 tggtagtggg cgggaagtcc atggttttgt agactttttt gaaaattatt ttttcattgt
     1921 atttttgtat aattcttttt tttcatcagc ttattttcat ggtaggcgag aagcctcgca
     1981 gtcttgcggg gcttgaccat gtccttaaat ttaaatttta tttattataa ttttagtatt
     2041 gttttcatgg taggcgagaa gccccgcagt cttgcggggc tcgatcatgt ccttaaattt
     2101 aaattttatt tattatgatt ttagtattgt tttcatggta ggcgagaagc cccgcagttt
     2161 tgcggggctt gaccatgtcc ttaaatttat atttatttat tatgatttta gtattgtttt
     2221 catggtaggt gagaagcccc gcagtcttgc ggggcttgac catgtcttta aatttaaatt
     2281 ttatttatta tgattttagt attgttttca tggtaggtga gaagccccgc agtcttgcgg
     2341 ggctcgatca tgtccttaaa tttaaatttt att
//
LOCUS       DAOSID010000110         2371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_38760_length_2371_cov_12.553541, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000110 DAOSID010000000
VERSION     DAOSID010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38760_length_2371_cov_12.553541"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1608
                     /locus_tag="PK674_03635"
     CDS             <1..1608
                     /locus_tag="PK674_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15654.1"
                     /translation="HPRTRPIIMDLYENPKRFLGMNDSISSGHLHQAKKPSNMVENFE
                     YLDFEAEDLVDCLPLGIYDQVGYFKPFEKQYLIDSSRCYPLDKVKNEIQAFIDSADQK
                     KISVIINSFNKMYPDNILSFKQWRNDKQNFIDDLLSKYDVDVFIGLFTSLGYQEFNKY
                     KNTKLMTAKISPKSDPNNRFNGFNCDSLAEGHGKKVVAMFNPYCTDFCIYEGEQDQNS
                     DNLKVTSWVTLNRKIPQNFSSLLEKAVSNTTHDIAQLLGKDFDDYKDPNEYIMTMDNI
                     EANPNFGNSYASAVRQMYEDFFSEYIKAHPTSPNGIPINTKHFQSGMSYNKIDFLHKT
                     IENNTIPVFVNAYTDNASSQSLKGDLHIEKQVEKQKKTGIHPLSIEDVIPISYLEGKI
                     YPEDLKSHIGNMQHEITASILNNTLKGRENLSFTRYDEEGKLRGYFLAYQGKMEDGRQ
                     GIYISDFAIDEKARGRSGVEMINNWIKQVKDHYPEMPVFARARESTSYRMVQVLAKKS
                     GYKITLDKKVQDAGEAFHRVVMEPVDS"
     gene            1967..>2371
                     /locus_tag="PK674_03640"
     CDS             1967..>2371
                     /locus_tag="PK674_03640"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOG15655.1"
                     /translation="MVPKRGVKRIPSLVVNRLARNLFKIRQFGKRELLHHQETFLFDL
                     IRKCRNTVFGQRYNFKNIKSIQDFQNNVPIFTYKEFKPRVEYMLRGEKNITYPGKIPL
                     FAVSSGTTGKEGKYIPLTNEHLKTSQMRGGEKL"
BASE COUNT          873 a          350 c          401 g          747 t
ORIGIN      
        1 catcccagaa caagaccaat cattatggat ttatatgaaa atcctaaaag atttctttga
       61 atgaatgata gtatttctag tgggcatctt catcaagcta aaaaaccttc aaatatggtt
      121 gaaaactttg aatatcttga ttttgaagca gaagatttgg tagattgcct tcctctatga
      181 atttatgatc aagttggata cttcaaacct tttgaaaagc agtatctgat cgatagctct
      241 cgttgttatc ctttggataa ggtcaaaaat gagatacagg catttattga ttctgctgat
      301 caaaaaaaga tttcagtgat tattaactca ttcaataaaa tgtatccaga caacatattg
      361 agttttaagc aatggagaaa tgataaacaa aattttattg atgatctctt gtcaaaatat
      421 gatgtagatg tttttatatg attatttact agtctaggat atcaagaatt caataaatat
      481 aaaaatacca agttgatgac tgccaaaatc agtccaaaat ctgatccgaa taaccgattt
      541 aatggtttca actgtgatag tttagctgaa tgacacggaa aaaaggtcgt tgcaatgttt
      601 aatccttact gtactgattt ttgtatttat gaaggagagc aagatcaaaa ttcagataat
      661 ctgaaagtga caagctgggt tacgctgaat agaaaaatac ctcaaaattt tagttcttta
      721 ttagaaaaag cagtatctaa taccactcat gatattgctc aattgcttgg aaaagatttt
      781 gatgactata aggatcctaa tgaatatatt atgaccatgg ataatattga agcaaatcct
      841 aacttttgaa atagctatgc ttcagctgtt cgtcaaatgt atgaggattt cttttctgaa
      901 tatatcaaag ctcatcctac ttctccaaac ggtattccaa tcaatacaaa gcattttcaa
      961 tctggaatga gttataataa gattgatttt ttacataaaa caatagaaaa taatactatt
     1021 cctgtcttcg taaatgctta tactgataat gcatcatctc aatcattaaa atgagatctc
     1081 catatagaaa aacaagtaga aaaacagaaa aaaactggaa ttcatccact ttctatagaa
     1141 gatgtgatcc ctatttctta tttagaatga aaaatttatc cagaagatct aaaaagtcat
     1201 atatgaaata tgcaacatga aattactgct tcaatattga ataacacttt gaaatgaagg
     1261 gagaatcttt cttttacccg atatgatgaa gaagggaagt tgagagggta ttttcttgct
     1321 tatcaatgaa aaatggaaga tggaagacaa tgaatttata tttcagactt tgcaatcgat
     1381 gaaaaagcaa gaggtcgttc gggtgttgaa atgataaata attggatcaa acaagtaaaa
     1441 gatcattatc cagagatgcc agtatttgct agagcgagag agtctacaag ctaccgtatg
     1501 gttcaagttt tagcaaaaaa aagtggatat aaaattactt tggataaaaa ggttcaagat
     1561 gctggtgaag catttcatcg agtagttatg gaacctgttg atagttaaaa ctaattacag
     1621 agccagagtt tgtgaatatt tctgcaatag aagataatag aaaatttaag ttatagattg
     1681 tcatttcgac taaatgaacg aagtgaacat acaggaacaa ggaattcttc cctcggggga
     1741 agttgctcta tcttagttgg gggttgttgg gggtatttgg attatttacc cccacctacc
     1801 ccgccccgta ggactacggg gcgggggaga acatcctccc cctagggagg gacttttctc
     1861 cccgctatcc ctcaaataca atttttcaaa catttttcca atacacttga tttttgggat
     1921 acaaaactta aaaattaatc tagttttttt atacattttt taaaaaatgg tcccaaaaag
     1981 aggagttaaa aggattccaa gtttagtagt aaatagacta gcaagaaatc ttttcaaaat
     2041 cagacagttt tgaaaacgag aacttttaca tcatcaagaa acttttcttt ttgatttaat
     2101 tcgtaaatgt agaaatacag tatttgggca aagatataat ttcaaaaaca taaaatctat
     2161 ccaagatttt cagaataatg tacctatatt tacctacaaa gaattcaagc ctcgagtaga
     2221 atacatgctt agatgagaaa aaaacattac ttatcctgga aaaattccat tatttgctgt
     2281 atcgtctggt acaacttgaa aagaatgaaa atatattcca cttacaaacg agcatttgaa
     2341 aacttctcaa atgagatgat gagaaaaatt a
//
LOCUS       DAOSID010000111         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_41177_length_2265_cov_16.402715, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000111 DAOSID010000000
VERSION     DAOSID010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41177_length_2265_cov_16.402715"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            564..>2265
                     /locus_tag="PK674_03645"
     CDS             564..>2265
                     /locus_tag="PK674_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG15656.1"
                     /translation="MSQKELINDLTRYWKEHKIFQKSIDQRSDTLKAITYDGPPFASG
                     EPHFGHGLVGVMKDLVGRYKTMKGYKVIRDRGRDCHGLPVEKAVEKKLGLDGKKDIEE
                     KLGIEKFTQECRAYVSNVSDEWSKFVDQTGRRADMENAYMTMNLDFMESVINVFDNLY
                     SKNLVYQGFNIQWYCPSCATALSNSEVNEGYEDRQDPAITIKFPIYNKNQEQLDKYEH
                     SSDGAINVVCCVIKRDDKFLIGYHKKGQKYVFPGGKIEKGDSIEETVIREVKEETGVE
                     VKSQKTIGEFKEILKGNIWNLIYVETEIGGEPQNMEPDKTTHFQRAEIIDSENELGMA
                     IKIENTIIDDAEEIINQFYDLYTYKNNFAGQITPDKDINILAWTTTPWTLPSNSFLAV
                     GPSIKYVLVFDKSSHEYYVIAEKLLNKYYKNKEEYLLINKFSGEDMKGIFYKPLFEYI
                     NQSDISDEYKKQYFQIIGGDYVSTEDGTGIVHIAPSFGQEDFEVVANIFPRENAKDWL
                     FLPVDDYGEFTDEVGDYKGIVVYDTNKDIVKRLKDEGKLIFQQSYTHSYPHCWRCHAP
                     L"
BASE COUNT          836 a          349 c          419 g          661 t
ORIGIN      
        1 aaatttttat caattttact tttttgttac cgaattacta aacctataca gggaccccgc
       61 tattcagccc cgctatttaa gcggggcgag cgggtggagg atattaaatc cctctacccc
      121 cgcaattcag cgggggagag caactccctt tacgaaaggg agacaatttt ttgtgacaaa
      181 agttcctccc ttgggggagg ctgaatgtcc cgaatttatg agggactagg tgggggtttt
      241 tacccccaac tacccccaac tacactagaa cagcttcccc cgagggaaga atttttttgt
      301 ctccatgcgt tcacttcgtt cacttagtcg aaatgacagg ctgtaaataa atccctctat
      361 ccccgcccgt agtactacgg ggagaacaac tccctttgaa aaagggagac aatttttctt
      421 ttccctcggc tttgctttat ttaccaacat aaacacgata aaaaaactca ctttcaattg
      481 aaaaactaag aaagattaat acatattaaa ttgatgtgtt ttttttattg ttatttttta
      541 cctcaatatt cattcaacaa aaaatgagtc agaaagaatt aatcaacgat ttaaccagat
      601 attggaaaga acataaaatt tttcaaaaat ctattgatca aagatccgat actctaaaag
      661 ctatcactta tgatgggcct ccttttgcta gtggagagcc acacttttga catggtttag
      721 taggagtgat gaaagatctt gtatgaagat acaaaaccat gaagtgatac aaggtaatca
      781 gagatcgagg acgagattgt catggtttac cagtagaaaa agccgtagaa aaaaaactat
      841 gactagatgg gaaaaaagat attgaagaaa aattgggaat cgagaaattt acccaagaat
      901 gtagagctta tgttagtaat gtgagcgatg agtggagtaa gtttgtagac cagacatgaa
      961 gacgagcaga catggaaaac gcttacatga ccatgaatct agattttatg gagtctgtaa
     1021 taaatgtttt tgataatcta tattcaaaaa atctagtata ccaatgattc aacatccaat
     1081 ggtactgtcc ttcttgtgct actgctttgt ccaattctga ggtaaatgaa tgatatgagg
     1141 atagacaaga tcctgctatt actataaaat ttcctatata caacaaaaat caagaacaat
     1201 tggataaata tgaacattca tcagatggag ctataaatgt agtttgttgt gtaatcaaaa
     1261 gagatgataa atttttgata tgatatcaca aaaaatgaca aaaatatgtg ttccctggtg
     1321 ggaaaataga aaaatgagat agtatcgaag aaaccgtaat cagagaggtt aaagaagaaa
     1381 cttgagtaga ggtgaaatct caaaaaacga tctgagaatt caaagaaata ttaaaatgaa
     1441 atatctggaa tttgatttat gtagaaactg aaattggatg agagccacaa aacatggaac
     1501 ctgacaaaac tactcatttc caacgagcag aaatcataga ttctgaaaac gagttgggga
     1561 tggctataaa aatagaaaat acaataatag atgatgcaga agaaataata aatcagtttt
     1621 atgatttgta tacctacaaa aacaattttg ctggacaaat aacaccagac aaagacataa
     1681 atattttggc ttggacgact accccttgga ccttgccttc aaacagtttt ttggctgtct
     1741 gaccaagcat taaatatgtt ttagtgtttg ataaatctag ccacgaatat tatgtaatag
     1801 cagaaaaatt gttgaataaa tattacaaaa acaaagaaga atatcttctg ataaacaaat
     1861 tttcatgaga agatatgaaa tgaatttttt acaaaccatt atttgaatat atcaatcaaa
     1921 gcgatatttc tgatgaatat aaaaaacaat atttccaaat aatttgatga gattatgtta
     1981 gtactgaaga tggaactgga attgttcata ttgctccatc atttggacaa gaggattttg
     2041 aggtagtggc aaatattttt cctagagaaa atgcaaaaga ttggttgttc ttaccggttg
     2101 atgactatgg agaatttaca gatgaagttg gtgattacaa atgaatagta gtttatgaca
     2161 caaataaaga tatcgtaaaa agattaaaag atgagtgaaa acttatattt caacagtctt
     2221 atactcactc atatccgcat tgttggagat gtcatgctcc actga
//
LOCUS       DAOSID010000112         2252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_41483_length_2252_cov_14.584888, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000112 DAOSID010000000
VERSION     DAOSID010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41483_length_2252_cov_14.584888"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1030)
                     /locus_tag="PK674_03650"
     CDS             complement(125..1030)
                     /locus_tag="PK674_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15657.1"
                     /translation="MNNKTKKTIETIKEEAKNYYKGKKMWPRYAIIGFLLLIIVLQFI
                     LPKQKKILVDEQVAEITGDITEENQENVFNFLGKDMDLDMNFEKSLVKILDKRNELLF
                     IYKYKTDILNKLGDELKEKDIPEEFRYFILLNQDENTIFPFEGDLNDNLIVDEEINER
                     LNYYKSLDNFVEYVKYLYNNFDDDRLVVIGYFMGLDNLKILMMEQSQTDFEKLYFPLD
                     VLEKYYNLMAYAYIYNNILGYIDIEDVSVVEYPETGKVRAGEIKDLVRRAKKAKYDFK
                     LIKELNPWILQNSLPKGKREITVPN"
     gene            1359..1850
                     /locus_tag="PK674_03655"
     CDS             1359..1850
                     /locus_tag="PK674_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="dCMP deaminase family protein"
                     /protein_id="HOG15658.1"
                     /translation="MLKKREDYISWDEYFMGIAMLSAHRSKDPNTQVGACIVNEKNRI
                     VGIGYNGFPRGCDDDTFPWDREGKLPDTKYAYVVHAEANAILNSGGRNLEGTKIYVAL
                     FPCNECAKLIIQAGIKEIVYLSDKYDGTDINIASKKMLNSAGIKLTKLDVKDKEILIK
                     LEA"
BASE COUNT          790 a          368 c          272 g          822 t
ORIGIN      
        1 ttttttcatt ttataagctt gttagttcaa acaaaaagtc tttactttta taaaagtatt
       61 gcaattagct aacactacga acagcacccg ggtggtagga tataattaac tttacgaact
      121 catcttagtt tggaacagta atttctcgtt ttcattttgg aagagagttt tgcaatatcc
      181 aaggattcaa ttcctttata agcttgaaat catattttgc tttttttgct cgtcttacta
      241 aatcttttat ttctcaagct cttacttttc atgtttctgg atattcaacc actgaaacat
      301 cctctatatc tatataccct aaaatattat tataaatata agcgtaagcc attaaattat
      361 aatatttttc caaaacatcc agtggaaaat atagtttttc gaaatcggtt tgactttgtt
      421 ccatcattaa tatttttaga ttatctaatc acataaaata tcaaattact accaaacgat
      481 catcgtcaaa attattatat aaatatttta catactctac aaaattatcc aaagatttgt
      541 aataattgag tctttcattg atttcttcat caactattag attatcattt aaatctcatt
      601 caaaagggaa aatagtgttt tcgtcttgat tgagtaaaat aaaatatcta aactcctctg
      661 gaatatcttt ttcttttaac tcatctcata atttgtttaa aatatcggtt ttatacttat
      721 agataaacaa aagttcgttc cttttatcca atatttttac caaagatttt tcaaaattca
      781 tatccaaatc catatctttt cacaaaaaat taaaaacatt ttcttgattt tcttccgtaa
      841 tatctcatgt aatttctgct acctgttcat ctactaggat ctttttttgt tttggtaaaa
      901 taaattgtaa cacaattata agcaataaaa atcaaataat agcatatctt ggccacatct
      961 tttttcattt ataataattt tttgcttctt ccttaattgt ttcaattgtt ttttttgttt
     1021 tgttattcat cagtaaaata taaataaata aagcattatt tttgtactta tatctcaaac
     1081 ttttgcaagc aattttattt ttcaatatta aaataacata ttaaaaaaca taagtataaa
     1141 tttaacttgt acttaacaaa ccacaaaaaa gacaaattct tcataattat ttccaccata
     1201 tcaaaaccac aactaaaaca tttatttaat aaagtaaatg cttaatttct tggctacata
     1261 aacacaataa taccaaataa gcgctaagtt tttactattg attttaatcc tatcttgttt
     1321 agaaaaaaag aaggaacttt tattttttca taaaaattat gctaaaaaaa agagaggact
     1381 atatttcttg ggatgaatat ttcatgtgaa ttgctatgct ttccgcacat cgtagtaaag
     1441 acccaaatac tcaagtagga gcttgtattg taaatgagaa aaatagaatt gttggtattg
     1501 gttacaattg attccctcgt ggatgcgatg acgatacttt tccttgggat agggaatgaa
     1561 aacttcctga taccaaatat gcttatgtgg ttcatgccga agcaaatgca attttaaata
     1621 gctgatgaag gaatcttgaa tgaacaaaaa tttatgtagc attatttcca tgtaacgaat
     1681 gtgcaaaact aattatacaa gccgggataa aagaaattgt atacctttca gataaatatg
     1741 actgaacaga tataaatata gcatcaaaaa aaatgctgaa ttctgcttga ataaaattaa
     1801 caaaactaga tgtaaaagac aaagaaattt tgattaagtt agaagcataa caacccgtaa
     1861 tcctgtatag ataagagaat ttacaaacct atcatttcaa gtgaagcttg tcatttttcg
     1921 actaagtgaa cgcacgaaga aatccacttt caataaattg tctcccttta tcaaagggag
     1981 ttgctctccc ctatttcttg cagccccgca attctgcggg gctacattat ttcatcattt
     2041 tatcatttta acatttcaac actttatcat ttattatcct accacccggg tgctgttcgc
     2101 acccgggtgg taggaaaatt tatcactccg cctagcggag cgaacttttc ttacatttcc
     2161 tctggagctt caataccaag tatcgacaat caatttttta aaacttgttt tactgcaaac
     2221 actaaagcca atgcagagtt tgaatcagta at
//
LOCUS       DAOSID010000113         2164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_43814_length_2164_cov_18.440019, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000113 DAOSID010000000
VERSION     DAOSID010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43814_length_2164_cov_18.440019"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..383
                     /locus_tag="PK674_03660"
     CDS             90..383
                     /locus_tag="PK674_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15659.1"
                     /translation="MNAEAILKPIGKGQITIPLSRRLHLGIDQKEVRAFLRGNQIVIE
                     PLNQEKIDRDVKQISIGELNDETQKAIEQSRKDYKEGKKEKFMSSKQVRNDIL"
     gene            370..657
                     /locus_tag="PK674_03665"
     CDS             370..657
                     /locus_tag="PK674_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOG15660.1"
                     /translation="MTSYKIIFDVNLLLKRDLNILKKNKKQIFHIKDKIEKYAQDPNK
                     FISQTQKLAPKQEGLYRMRVGKYRIIFSKDDGNKILIIHRIGTRGDIYKKL"
     gene            849..2159
                     /gene="hisS"
                     /locus_tag="PK674_03670"
     CDS             849..2159
                     /gene="hisS"
                     /locus_tag="PK674_03670"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="histidine--tRNA ligase"
                     /protein_id="HOG15661.1"
                     /translation="MLSTQPAKGTQDRYPQEFQIRKYIFDSWRKVCLKFGYQEYLGPL
                     VENAEIRKAKSGEDVGGSELTMITNREGKISELALRPEMTPTVTRMVAKNYAQLAKPI
                     RYFSIANFYRNERPQRGRNREFRQLNVDMFGSDSIYADVEILTLAISLMLEFNPPKGS
                     WKLNINNRFIIDSILSEIGLSGDIKQETVRLMDKREKLPQEAISEILLEKGLNQNQVN
                     MLVEYMNSSTIQDLIKKFPSIQDSKGIQQTNFIIQSLNNLGYSEYLEFKPSLIRGFDY
                     YDGVVFEIFDTHPDNKRALFGGGRYNGLSKIFIKDDIPAIGFAPGDETTKLFLESRGL
                     IDPILNKTQIEKIYIPVLEDSLQDEVFKLAMNFRNYGKNVITGLETQKMIKAIQYADK
                     QDYKFIVIFGEEEKEKGIYKVKNLKSGKEKEFKLNEDFSVEELN"
BASE COUNT          916 a          258 c          323 g          667 t
ORIGIN      
        1 ttttaaaatt gataatttat ttgcatttta ataaaaaata actaaacttt aactagttta
       61 attattttaa ctagtttaat taaaataaca tgaatgcaga agctatatta aaaccaatag
      121 gtaaatgaca aattactatt cctctatctc gaagacttca tttatgaata gaccaaaaag
      181 aagttagagc atttttgaga tgaaatcaaa ttgttataga accacttaat caagagaaaa
      241 tagatcgaga tgtaaagcaa atttcaattg gtgaattaaa tgatgaaaca caaaaagcaa
      301 tagagcaatc tcgtaaagat tataaagaat gaaaaaaaga aaaatttatg tcgtcaaaac
      361 aagtgcgaaa tgacatctta taaaataatt tttgatgtaa atctattatt aaaaagagat
      421 ttaaatattt tgaaaaaaaa taaaaaacaa atttttcata taaaagataa gatagaaaaa
      481 tatgcacaag atccaaataa atttatttct caaacacaaa aactagcacc aaaacaagag
      541 tgattatata ggatgagagt tggaaaatat agaataattt tttcaaaaga tgattgaaat
      601 aaaatactta ttattcatag aattgggaca agatgagata tttataaaaa gttataaagt
      661 aagaaatagt atttattcat gactattaat taaaaaccct ttttataaca aaattattgt
      721 tgattatagt tatgttttcc atatacataa ttctatagga taggggactt attacggcga
      781 ctgcctctat aagcacaatc acaaatatat aaagcttata cgccatttta gttattattt
      841 tcaaaattat gctttcaaca caaccagcaa aatgaacaca ggatcgatat ccacaggaat
      901 ttcaaatccg taaatacatt tttgattctt ggagaaaagt ttgtctgaaa ttttgatatc
      961 aagaatactt atgacctttg gtagaaaatg ctgaaattcg aaaagcaaaa tctggcgaag
     1021 atgtttgatg atctgagctt acgatgatta caaataggga atgaaaaata tcagaacttg
     1081 ctcttagacc agaaatgaca cccacagtga ctcgtatggt ggccaaaaat tatgcacaat
     1141 tggcaaaacc tatcagatat ttcagtatag ctaattttta tcgtaatgaa agacctcaga
     1201 gaggtagaaa tagagaattt cgacaactaa atgttgatat gtttggatca gacagtattt
     1261 atgctgatgt tgaaatcctt actttagcta tatctttgat gttggaattt aatccaccaa
     1321 aatgatcttg gaaactaaat ataaacaatc gtttcatcat agattcaata ctatcagaaa
     1381 tttgattatc tggagatata aaacaagaaa cggttaggtt aatggataag cgagaaaagt
     1441 tgccacaaga agcgataagt gaaatattat tggaaaaatg attaaaccaa aatcaagtaa
     1501 atatgttagt ggaatatatg aattcaagca caatccaaga cctaataaaa aaattcccat
     1561 ccattcaaga ctctaaatga attcaacaaa caaactttat tattcaatca ttaaataatt
     1621 tatgatattc tgaatattta gagtttaaac caagcctaat aaggtgattt gactattatg
     1681 atggagttgt ttttgaaata tttgataccc atccagataa caaaagagct ttgtttggag
     1741 gatgaagata caattgatta tcaaaaatat ttataaaaga cgatattcca gccatttgat
     1801 ttgcacctgg agatgaaact acaaaattat ttttagaatc tcgatgacta attgatccta
     1861 ttttaaataa aacacaaatc gaaaaaatat atatccctgt gttggaggac tccttgcaag
     1921 atgaggtttt caagctagca atgaatttta ggaattattg aaaaaatgtt attacaggat
     1981 tagaaacaca aaagatgatc aaagctattc aatatgcaga taagcaagat tataaattta
     2041 ttgtaatttt tggagaggaa gaaaaagaaa aatgaattta caaagtaaaa aatctaaaaa
     2101 gttgaaaaga aaaagagttt aaattaaacg aagatttttc ggttgaagag ttaaattagt
     2161 aaaa
//
LOCUS       DAOSID010000114         2137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_44570_length_2137_cov_12.376561, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000114 DAOSID010000000
VERSION     DAOSID010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44570_length_2137_cov_12.376561"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            380..1372
                     /gene="holA"
                     /locus_tag="PK674_03675"
     CDS             380..1372
                     /gene="holA"
                     /locus_tag="PK674_03675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOG15662.1"
                     /translation="MIKNLYFFTGDEEYLLDQELYRRVSNFTQKFGNDSVSFFHVENW
                     DEGKAKQSIYGGGLFSTKTLTVIDGLPTGTDRFGFTISQVENFIDEFLKKKGEIPAEN
                     LLVFANSKPDKRSRLYKFLKDNANIKEFKTMDNAGLKVYIKQELGNLKISDSVLQKFI
                     QKVGVELYRINSEIDKLKYYCEYNSLSEITENLVDEVVFGLVDSQVFELLDYLLKDKT
                     KAIKFLQKMQDQGLNRNAISGVLYWSLRLYITVYHFAKNGITDLKTIANKTGINSYSL
                     HQNVKNIDLIQSNGTRLENMYKELIDLDFKIKSGKAEEESFWLETKKLINSFKL"
     gene            1379..1975
                     /locus_tag="PK674_03680"
     CDS             1379..1975
                     /locus_tag="PK674_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15663.1"
                     /translation="MKKKFFVLNIIVVGFFLGYSFANNMKDNFFCVVSPNAITVTIEK
                     ENNYKCSTYLDVLSQAISKEYKDFLEIQDIIEQGYDVDFWTTIRNDKIERIKKILLVR
                     EQIEEAVISFNNNMFDKIKEYLIFSVSPYHLKYKRFAKSFKQFENSRTLPLNVRNMIT
                     YLKEQVQVIENILTAEDYDVLIKNFNRYVYLKKQIEGK"
BASE COUNT          810 a          210 c          369 g          748 t
ORIGIN      
        1 atttgaatta tcttcacgat agacttatta ttacaaaacc agatccaaca ataactgaat
       61 tgagagcttg gattttgttg atgctgtata gaagttagtt ggaaagttca taaagttgga
      121 aagtttataa agttaaagta acaagtttat caaattctat gttgaggaca taattggcaa
      181 caatggcctc ttgttaaaaa agtgcgaatt tgagaaacag agaggaaata gtaggggatt
      241 tctttgtgtg ttcacttagt agaaatgaca tgcttcgctt gaaataatac aagggtgaaa
      301 aaaaattttt taattagttt gaatttgata ggtaaaaaag tattattcac tttgtaaact
      361 tttatttgga cattaattta tgataaaaaa tctatatttt tttacttgag atgaagaata
      421 tcttttggat caggaacttt atagacgagt ttccaatttt actcagaaat ttgggaacga
      481 ttctgtttct ttttttcatg tagaaaattg ggatgagtga aaagcaaaac aatcgattta
      541 tggttgaggt ttattttcta ctaaaactct taccgtcata gatggtcttc ctacttgaac
      601 ggataggttt ggatttacga tatctcaggt ggaaaatttt atagatgagt ttttgaaaaa
      661 aaaatgagaa attcctgctg aaaatttact tgtttttgca aactccaaac ctgataaaag
      721 atctagactg tataaattcc taaaagataa tgccaatata aaagaattta agaccatgga
      781 taatgcttga ttaaaagttt atatcaagca agaattgtga aatcttaaga tttctgatag
      841 tgttttacaa aagtttattc aaaaggtgtg agtagagttg tatcgtatca attcggaaat
      901 agacaaatta aaatactatt gtgaatataa ttccttatca gaaataactg agaatttggt
      961 tgatgaggtg gttttttgac ttgttgattc tcaggttttt gagttattgg actatttgtt
     1021 aaaagataaa acaaaagcaa taaagttttt acaaaaaatg caagatcagt gattaaaccg
     1081 aaatgcgata tcaggggtgt tgtattggtc gttgagattg tatattactg tttaccattt
     1141 tgcgaaaaat ggtattacag atttaaaaac tattgcaaac aaaacatgaa taaactctta
     1201 ttctttgcat cagaatgtga aaaatattga tttgatacaa agtaattgaa ctaggttgga
     1261 aaatatgtat aaagagttga tagatttgga ttttaagata aaaagtggta aagcagagga
     1321 agaatctttt tggttggaga caaaaaagct aattaattca tttaaacttt aatatcatat
     1381 gaaaaaaaaa ttctttgttt tgaatattat agttgtgtga ttctttttgg gctactcttt
     1441 tgctaataat atgaaagata attttttttg tgtagtttct ccaaacgcta ttactgttac
     1501 tatcgaaaaa gaaaataatt ataaatgttc cacatatttg gatgttttat cccaagcgat
     1561 tagtaaagaa tacaaggatt ttttggaaat acaagatatt attgaacaag ggtacgatgt
     1621 agatttttgg acaacaataa ggaatgataa aatagaaaga ataaaaaaaa tattattggt
     1681 aagagaacaa attgaagaag ctgtaataag ctttaacaat aatatgtttg ataaaataaa
     1741 agaatattta atattttcag tatctccata tcatttaaaa tataaaagat ttgcaaaatc
     1801 cttcaaacaa tttgaaaaca gtaggacctt gcctttgaat gttagaaata tgataacata
     1861 tttgaaagaa caggttcaag taatagagaa tattctaaca gcagaggatt atgatgtgtt
     1921 gataaaaaac tttaatagat atgtttattt aaaaaaacaa attgaatgaa aataggtgtt
     1981 atttcttcag gaaacgaagt tttgacttta tttaagtttt tggcgaaatt tgataatcaa
     2041 tatcttattt attatgatca agtaaatcga ccttatggcg acaatacttt tgaatattca
     2101 ttggattgtg tacaaaaatg aatagatttt ttgaaaa
//
LOCUS       DAOSID010000115         2121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_45062_length_2121_cov_17.097773, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000115 DAOSID010000000
VERSION     DAOSID010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45062_length_2121_cov_17.097773"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PK674_03685"
     CDS             complement(<1..720)
                     /locus_tag="PK674_03685"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HOG15664.1"
                     /translation="MKFKNKVAIVTGSSRGIGKATALELAKNGASVVISSTKLENCTK
                     IVKEIEDFGGKAIAVKCDVSNSKDVKNLVDTTIKNFGKIDILVNNAGIGPTKAFEELT
                     EEERDFVININLKGVFLCTNNVIKYMLKQKSGKIISISSILGKRGFAHASSYCTTKAA
                     VINLTRSLAIEYASRNININAVAPGFIRTDMTKESLENDEVSKMLLNHTPAGRFGEAE
                     EIAKAVAFLASDDASYIHGETL"
     gene            1147..1914
                     /locus_tag="PK674_03690"
     CDS             1147..1914
                     /locus_tag="PK674_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOG15665.1"
                     /translation="MFGLLFFLYSKDYTNFYNGDGFDQQEGTGINVLYSNILYKNHNY
                     TGILDWINKYDADVVLMVEFADEHYQNMKENLYDKYPYSARTSWSQKYFGNVVFSKYP
                     IDNLTDNVDQGTWRYSHFNLKQDDKNYYFYLVHTSSPAAYKFFTMRNKQFNILMNDFE
                     KHDLEQSQNNHVMVLGDFNVSPWSIYYKKLEAVMSGFKNMTKNFRILFTWRIKSLPFI
                     ISHIDHIFIDDDSVVSNITKLNTPGSDHRGFFIENFR"
BASE COUNT          662 a          270 c          286 g          903 t
ORIGIN      
        1 taatgtttct caatgaatgt aactagcatc atctgaagct aaaaatgcta ctgcttttgc
       61 aatttcttct gcttctccaa accttcatgc tggagtatgg tttagcaaca ttttacttac
      121 ttcatcattt tccaagcttt cttttgtcat atctgtcctg ataaatcatg gtgctacagc
      181 atttatatta atattgcgtg atgcgtactc tattgccaaa gatcttgtta gattgattac
      241 agctgctttg gttgtacaat atgatgatgc atgagcaaat catctttttc ctaaaataga
      301 agatattgaa ataatttttc aacttttttg ctttaacata tattttatta cattatttgt
      361 acataaaaat actcatttta gatttatatt tatgacaaaa tctcgttcct cttctgttag
      421 ttcctcaaag gcttttgtag gaccaattca tgcattgttg accaaaatat ctatttttca
      481 aaaattttta atagttgtgt cgactaggtt ttttacatct tttgaatttg aaacatcaca
      541 ttttactgct atagcttttc aaccaaaatc ttcaatttct tttactattt ttgtgcaatt
      601 ttctagtttt gtacttgaaa ttactacact tgctcaattt tttgcaagct ctaaagcggt
      661 tgcttttcag attcctctac tggaaccagt aactatggct accttgtttt taaatttcat
      721 gttgttattt tttaaggaat aaagtttttt attttatcag ttttgaatac tttattttaa
      781 taaatatttg tttctacgaa tgatagtaat atataaaaga acattattaa atcaaatgga
      841 aaagaatact tattattttg gtactttttt gtttttaact tgcaaaaaaa tcttgctttt
      901 ttataagtat tttcgttatg attttagttt ttattttgaa caatgtctta tgttatttta
      961 acattttttg tttttgtatt attgtttttt acatatttat ttcctggttt ttattttagt
     1021 gaattggttt taagtttttt gccatatatt gttcttttta caatccttac ttttgcaact
     1081 caatttgtct tattaaaaaa taaaaaggtt tgaatttatg ctaaattctc acccttgttt
     1141 attttaatgt ttggtctttt attttttctt tattccaaag actacacaaa tttttataat
     1201 tgagattgat ttgatcaaca ggaatgaact gggataaatg ttttgtattc aaatattttg
     1261 tataaaaatc acaactatac tgggattttg gattggataa ataaatatga tgctgatgtg
     1321 gttttgatgg tagaatttgc tgatgagcat tatcaaaata tgaaagaaaa tttgtatgat
     1381 aaatatcctt actctgctcg tactagttgg tcacaaaaat atttctgaaa tgttgtcttt
     1441 agtaaatatc caattgataa cttaactgat aatgtagatc aatgaacatg gagatattca
     1501 catttcaact tgaagcagga tgataaaaat tattattttt atttagtaca tacgagttct
     1561 cctgcagctt ataaattttt tactatgaga aataaacaat tcaatatatt gatgaatgat
     1621 tttgaaaagc atgatttgga acaatcacaa aacaatcatg taatggtttt gtgagatttc
     1681 aatgtttctc cgtggtctat ttattataaa aaattagaag ctgtaatgtc tggattcaaa
     1741 aatatgacaa aaaattttag gatactattt acttggagaa taaaatcatt accttttatt
     1801 atatctcaca ttgatcatat atttatagac gatgattctg ttgtgtcaaa tattaccaaa
     1861 ttaaatactc caggttcaga tcatagatga ttctttattg aaaattttag ataatttaat
     1921 tctttgaaat gtttcaattt atttgggatt ttgttcgtta tccgtttgat caagataaag
     1981 aaaaaaataa attttctaag tttttgaatt tgttgttgaa ggactaccct gcttttttgt
     2041 ggttcaaacc tacttggcaa aggattgtag tttctccact tttgtttatt gcaaaaattt
     2101 ttcagaaaaa aaagtagttt t
//
LOCUS       DAOSID010000116         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_47725_length_2034_cov_13.948964, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000116 DAOSID010000000
VERSION     DAOSID010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47725_length_2034_cov_13.948964"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="PK674_03695"
     CDS             complement(<1..1098)
                     /locus_tag="PK674_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15666.1"
                     /translation="MANKKNTLDVKVKKINIHDGEKLIVVLNEIDAVEYGLNAFDKVK
                     IIFGKEIIVANLDITKKLVKPGEIGVFEDTFAKANFNEGDIVRVEYTKRSNKTVEAIR
                     KKMQGKSISENEINSIIKDISQNKLVDSLMTYYVASSFFYPTSDKETYLTAKAMAENG
                     VMFKYPKGTIVADKHCIGGVPGNETTMILIPLIASLGIKIPKNFSKAITSPAATGECV
                     NVLMDIEFNEQEIKKLVKELNCCLVWGGGLDLAPADDKLIQIQHPLSMQNISKVVSSI
                     MAKKYAMGITHLLIDVPMGPTAKVKNMKEAKEWEEKFNTLGKLLGIKMCVAITEAKEP
                     IGAGIGAVLQVREVLRVLQQHPKRPMDLEKKT"
     gene            complement(1126..1593)
                     /locus_tag="PK674_03700"
     CDS             complement(1126..1593)
                     /locus_tag="PK674_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15667.1"
                     /translation="MNNKTKINLDEITYEEYLNDDIYNIDPGGDISDGGEYVDFLIEN
                     LTKEPIPREVPEEYIDQIVKDEDYMEFRAYLERIADDTELIKKVMEYVEFNYPEFFED
                     SQVANNEKIPKQNIRADKPLNKKIPSDLGSEEGKLTHRQLTQTGTEIQDVLTW"
     gene            complement(1689..>2034)
                     /locus_tag="PK674_03705"
     CDS             complement(1689..>2034)
                     /locus_tag="PK674_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=25
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HOG15668.1"
                     /translation="PEHDEDLIEKIQMDNQFVLTNEDDSWRTIKKCCPIISGGLNPTL
                     LKPFIDLMGNVDFITTMGAGCNAHPKGTKVGAKALVQACEAFQKGIDIHDYAKEHEEL
                     AEAIEFFEKKKK"
BASE COUNT          620 a          371 c          230 g          813 t
ORIGIN      
        1 tgttttcttt tctaagtcca taggtctttt tggatgttgt tgcaaaactc taagaacctc
       61 tcttacttgc aatactgctc aaattcatgc tcaaattggc tctttagctt ctgtaattgc
      121 aacacacatc tttattccaa gtagttttca aagtgtattg aatttttcct cccactcttt
      181 agcttctttc atgtttttaa cctttgctgt tggtcacata ggcacatcta taagcaaatg
      241 agtaattcac attgcatatt tttttgccat tatagaactt acaacttttg aaatattttg
      301 catactcaaa ggatgttgta tttgaattag cttatcatca gctggtgcta aatctaaacc
      361 tcatcaccat accaaacaac aattcaattc tttaactaat ttttttattt cttgttcatt
      421 aaattcaata tccatcaaaa catttacaca ctcacctgtc gcagcaggag aagtaatagc
      481 ttttgaaaaa tttttcggta ttttaattca taaagatgca attagtggaa ttaagatcat
      541 tgtggtttca tttcacggta cacctccaat acaatgttta tcagcaacta tggttcattt
      601 tggatattta aacataactc aattctcagc catagctttt gcggttaagt atgtttcttt
      661 atcactagta ggataaaaaa agctactagc aacatagtaa gtcatcaagg aatcaactaa
      721 tttattttga gaaatatctt taataataga attgatttca ttttcactaa tagactttca
      781 ttgcattttc tttctaatgg cttcaactgt tttatttgac ctttttgtat actccactct
      841 cacaatatct cactcattaa aatttgcctt agcaaaagta tcttcaaaaa ctcagatttc
      901 tcaaggtttt actagttttt tagtaatatc caaattcgcc acaataattt cttttccaaa
      961 aattattttt actttatcaa aagcattcaa cccgtattct acagcatcta tttcatttaa
     1021 aacaacaatt aatttttcac cgtcatgaat gtttattttt tttaccttta catccaatgt
     1081 gtttttttta ttagccatgt tttttttcta ttaagttaaa taaaactacc aagtaagcac
     1141 atcttgtatt tctgtacccg tttgagttaa ctgtctatgg gttaattttc attcctcaga
     1201 tcataaatca gaaggaattt ttttattcaa aggtttatct gcccttatat tttgtttagg
     1261 aattttttca ttgttagcta cttgactatc ttcaaaaaat tcaggatagt taaattctac
     1321 atattccatt acttttttta tcaattcggt atcatccgct atcctttcca aataagcccg
     1381 aaattccata taatcctcat cttttacaat ctgatcaata tattcttcag gaacctctct
     1441 aggaattggc tcctttgtta aattttctat taaaaaatct acatactctc atccatcaga
     1501 gatatctcaa ccaggatcaa tgttataaat atcatcgttt aaatattctt cataagttat
     1561 ttcatcaaga ttaatttttg ttttattgtt cattttttta taataattta tattttaaaa
     1621 gagagagtgg aaagtaggat gtaagaaatg ataaataatt atcaaacacc actaccccac
     1681 ctatttttct attttttctt tttttcaaag aattctattg cttctgctaa ttcttcatgc
     1741 tctttggcat aatcatgaat atctattcac ttttggaaag cttcacaggc ttgcaccaaa
     1801 gctttcgcac caaccttggt cccttttgga tgtgcattac aaccagctcc catagtggta
     1861 ataaaatcaa catttcacat aagatcaata aatggtttta ataatgttgg attaagtcat
     1921 ccagatataa ttggacaaca tttttttata gttctccaac tatcatcttc atttgttagt
     1981 acaaattgat tatccatttg tattttttct atcaaatctt catcatgttc cggt
//
LOCUS       DAOSID010000117         2006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_48610_length_2006_cov_13.334700, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000117 DAOSID010000000
VERSION     DAOSID010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48610_length_2006_cov_13.334700"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            513..1856
                     /locus_tag="PK674_03710"
     CDS             513..1856
                     /locus_tag="PK674_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15669.1"
                     /translation="MQKISIQDSYFNILEMLDQQPGSEQILNALIQLESDIESAVFDI
                     LKNYQAQNKKINNTKIYQAMHSRNFELRNVLESRIMQIATGDKMSISDLLDQCIAHLN
                     KILDNQENMEDILKKMKICYKKVEDTILPPDEKPIQTGSGNGWIEKREGYNKFGLLLN
                     LLREKGIYTDDLYVAIGVNQKNQMREQSYLYIYIPRINKTILINNSYGEKTFIYNGYI
                     QLNYFLGNTKIGIKNNENIVGVNFDYENITNWKSKMNYYLFEKQETDKPLIGEKINVE
                     KFVKRQDFVDFYECLSDEEKNMAIKGAHEYEKLAENYPNIPKTYGGLIPLLGGTVKCW
                     RGGYVEALLKGDEEGKKYYEERKKINSKKIRTREGFLDFYERLSEAERKMVVGSFAKY
                     KELAKTYLNIPKSYEGIISLLGGNNKCLGGGYVEALLKGEEEGKRYYEERKKMKK"
BASE COUNT          783 a          243 c          310 g          670 t
ORIGIN      
        1 gtagtttctc cacttttgtt tattgcaaaa atttttcaga aaaaaaagta gttttttaac
       61 atttatgaat tttatttttg aattttttat tcatcagctt gtttgaaatt cttaatattt
      121 tttctcactt cgtcagaata cccagcttca attaatcttt tcgccatcct cagatctagt
      181 cattcaaatt tatcaagatt gtcagctaga taccatgact ccccaatttt aatcgctact
      241 ttcgcgatct cattatggtc aaatctttta aatttatcaa gggtttatca actttgtaaa
      301 gttgaaaata ttttaccatc taggtgatta aataccatct gttgagtgct aaagaatagt
      361 atgtgtgatg gatgatctgg gtggtaaaat tatattttat tttattattt ttcgcttgat
      421 tttttgtgta aaatatatat tatatgtgat gtgtgtctac atgaatttat taaaagcaaa
      481 actatttctt ttatgttttt agaccttata ttatgcaaaa aatctctatt caagactcat
      541 attttaatat tttggaaatg cttgatcagc aaccatgatc cgagcaaata ctcaatgcat
      601 tgattcaatt ggaatcagat attgaatctg cagtttttga tatattaaaa aattatcaag
      661 cacaaaacaa aaaaatcaat aataccaaaa tatatcaagc gatgcatagt agaaatttcg
      721 agttaagaaa tgttttggaa tccagaatta tgcaaatagc aacgggcgac aaaatgagta
      781 ttagtgattt actggatcag tgcattgctc atcttaataa aatactagat aatcaagaaa
      841 atatggaaga tatactaaaa aagatgaaaa tttgttacaa aaaagtcgaa gatacaattc
      901 ttccgccaga tgaaaagcca atccaaacag gttcttgaaa ttgatggatt gaaaagagag
      961 aatgatacaa caagttttga ttgttgctta atttacttag agaaaaatga atttataccg
     1021 atgatttgta tgtggctatc ggagtaaatc aaaaaaatca gatgagagag caaagttatc
     1081 tttatattta tattcctaga atcaataaaa caattcttat caataattct tacggagaaa
     1141 aaacctttat ttataattga tatatccaat tgaattattt tttgggaaac acaaaaatct
     1201 gaatcaaaaa taacgaaaat atcgttggtg tcaattttga ctatgaaaat attacaaact
     1261 ggaaatccaa gatgaattat tatttatttg aaaaacaaga aacagataaa cccctaatct
     1321 gagaaaaaat taatgtagaa aaatttgtaa aaagacaaga ttttgtagat ttttatgaat
     1381 gcttatcaga tgaagaaaaa aatatggcta tcaaaggtgc acatgaatat gaaaaattag
     1441 cagagaatta tccaaacata cccaagactt atggatgact aattccctta ttatgatgaa
     1501 cagttaaatg ttggaggtga tgatatgtag aagcattatt gaaatgagat gaagagtgaa
     1561 agaaatatta tgaagaacga aaaaaaataa attctaaaaa aataagaaca agagaggggt
     1621 ttttggattt ttatgaacgc ttatcagaag cagaaaggaa aatggttgtt ggcagttttg
     1681 ctaagtataa agaactagca aagacttatt taaacatacc caagagttat gaatgaataa
     1741 tctccttatt atgatgaaat aataaatgtt taggatgatg atatgtagaa gcattattga
     1801 aatgagagga agaatgaaag agatattatg aagaacgaaa aaagatgaaa aaataaaaaa
     1861 ataaatcgca aattacggaa actctcccaa agctttcata catacaacag cccctaacca
     1921 gaggttgttt tataaattta ttcaaaaaac ttttttcatt ttaatttatc tggtaagtat
     1981 tgttgttcta cttttgagtc tttgta
//
LOCUS       DAOSID010000118         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_50013_length_1965_cov_16.871204, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000118 DAOSID010000000
VERSION     DAOSID010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50013_length_1965_cov_16.871204"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PK674_03715"
     CDS             complement(<1..1171)
                     /locus_tag="PK674_03715"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="replication-associated recombination protein A"
                     /protein_id="HOG15670.1"
                     /translation="MLEPLSSLLRPQKIEDFAGQKDILLPISSFLDSKYIPSMLFWGP
                     AGTGKTTLARIIAKKMDAQFRELSGVISKKEDLVEIIKQAKINFSFAKASIVFLDEIH
                     RRNKAQQDTLLPFVEKGIITLIGASTENPGFYINNALLSRCRTFVFKQLEPEEIFDFL
                     KKNLKKIYQKYPEIQINDEVLTLISKLANGDLRTGINILEASILYKGKGKLNLDDVQK
                     NTQKNIYYDRDGDEHYNVISAFIKSMRGSDVDATAYRLQRMIKAGEDPLFIARRMIIL
                     ASEDIGLANSNALLIANQAFDAVQKVGFPEAEFALFHAAIYLAKSAKSNAVYKSILDT
                     RKAVEEFGNLEVPIHLRNSTSKLTEELGYGAEYKYPHDYKDSKVEQQYLPDKLKGK"
     gene            complement(1314..>1965)
                     /locus_tag="PK674_03720"
     CDS             complement(1314..>1965)
                     /locus_tag="PK674_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15671.1"
                     /translation="GTVYVGEPEFTESKFYDIFIGKEKNMNFELDYKENDLPPTFHKR
                     KSEQTPAKINVIIKDIKQIILPEFTEENIKKFFPDQKEVENLQQLKEYIKNEIEKQKH
                     DSELIKNIEEYINNIRDKSMKITIPQTLIRQEFKSRIDNLEQRFGGQEKLTEYFKQLG
                     DEKTKQFVEDISKASQDSLEKFFILQKITEELKIDIDRQKAGKLEVEQKLYDKVSK"
BASE COUNT          525 a          292 c          277 g          871 t
ORIGIN      
        1 tttttcattt taatttatct ggtaagtatt gttgttctac ttttgagtct ttgtagtcat
       61 gtggatactt atattctgct caatatcata attcttctgt aagtttgcta gtagagtttc
      121 ttaggtggat gggtacttct aaatttcaaa actcctctac tgcttttctt gtatctagta
      181 tacttttata tactgcattt gactttgctg acttagctaa gtatattgct gcgtggaata
      241 atgcaaactc tgcttcaggg aatcaaactt tttgtactgc atcaaaagct tgatttgcta
      301 tcaataatgc gtttgaattt gccaaaccaa tatcttcact agcaaggatt atcattcttc
      361 tagctataaa tagtgggtct tctcatgctt ttatcattct ttgtaaccgg tatgctgtcg
      421 catcgacatc actaccacgc attgatttga taaaagctga tattacatta taatgttcat
      481 ctccatctct atcataataa atattttttt gtgtgttttt ttggacatca tctaaattta
      541 attttcattt tcatttatat aaaatacttg cttccaatat gtttattcaa gttctgaggt
      601 ctccgttagc tagtttggaa attagagtca gtacttcgtc atttatttgg atttctggat
      661 atttttggta aatttttttt agattctttt ttagaaaatc aaatatttct tctggctcta
      721 gctgtttgaa tacaaacgtt ctacatcttg ataacaaagc attgtttata taaaatcatg
      781 gattttcggt gcttgctcat attaatgtaa taattcattt ttctacgaat ggtaataatg
      841 tatcttgttg tgctttattt cgcctgtgta tttcgtccaa aaatactatt gatgcttttg
      901 caaaactgaa atttattttt gcttgtttta tgatttctac taaatcttct tttttactta
      961 taactcaaga taattctcga aattgtgcat ccatcttttt tgctataatt ctagctaatg
     1021 ttgtttttca ggttcatgct ggtccccaaa aaagcataga tggaatatat ttactatcca
     1081 aaaaactaga tataggtagt aatatatctt tttgtcctgc aaaatcttct attttttgtg
     1141 gtcttaatag acttgataat ggttctagca tttgtggttt gtaaagttat agccccttaa
     1201 ctacggagtt ctccgtaacg agtaagggga tacttatcag cttttaataa atccccctta
     1261 atccccctta ctaaggggga cattcaaaaa ctttataaac tatttatttg taactactta
     1321 ctaaccttgt cataaagttt ttgttccact tctaattttc atgctttttg tcgatctata
     1381 tctattttta gttcttctgt gattttttgt aagataaaga atttttctaa actatcttga
     1441 gaagctttgg atatgtcttc tacaaattgt tttgtttttt catctcataa ttgcttgaaa
     1501 tattctgtta gtttttcttg acctccaaat ctttgttcta aattatctat tctagattta
     1561 aactcttgac gaattaatgt ttgagggata gtaattttca tgcttttgtc tctgatatta
     1621 tttatatact cttctatatt tttaatgagt tcggaatcgt gtttttgttt ttctatttca
     1681 tttttgatat attcttttaa ttgttgtaga ttttctactt ctttttgatc tgggaaaaac
     1741 tttttaatat tttcttctgt aaattctggt aatatgattt gtttgatgtc tttgatgata
     1801 acattaattt ttgcaggggt ttgttctgat tttcttttat ggaaggtggg aggtaaatca
     1861 ttttctttgt agtctaattc aaaattcata tttttttctt ttcatataaa aatatcataa
     1921 aatttagatt ctgtaaattc tggttctcat acataaactg tacca
//
LOCUS       DAOSID010000119         1953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_50441_length_1953_cov_18.955743, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000119 DAOSID010000000
VERSION     DAOSID010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50441_length_1953_cov_18.955743"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1953
                     /locus_tag="PK674_03725"
     CDS             <1..>1953
                     /locus_tag="PK674_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="AAA family ATPase"
                     /protein_id="HOG15672.1"
                     /translation="NIYIQKIILDLLAISYQNIGPFKDKIINIFFDKGKFLIKAPIGS
                     GKSFLFFDGPVYALYKNTGRNVLNVDSKNGFIKLLFEINGQKYLIVRNLTKGPVKDSC
                     TSQLFLVHNLIGGVSKESDQDIHEILKKTDGVNLEEIVFKNERDLEGNLSTILPPREA
                     FLSTMFLLQDSENIFELQAAERLTVMKNIFNLIGIDEIKDQIANKRRDIQTTLKVKSD
                     TSNYDLKIKNILQNLIQNFKKIDIPETDENLKTALSNNQELIQERELIADNINIDGFS
                     VQSFDKNLNTELNSHIGKQKSSYQEFFVKLQHTQEQTKNKQENIKILNNDLIDFQKQI
                     ENFDKQIENIDPEIIDNKKKEKINLLKESDKILDGVNLQHDIPNEIHEFISKKPNDIE
                     DWNNLVIELTTKGQDLANQKKNTELMVQNKKLKIKSENEKKEIEIKNLNETQKTLKNN
                     LNNIVDRINNLNKSINEESQYKCSQINDFCPFIKEINKQTFQELEKQKESFEKEKQEL
                     ESKIAEIQERIQNNNKINPDQELEQNSIENLENEIKKIEESINNLKTFFTKIEFKKIQ
                     ETYNQRKNIQTEISKLDKEILQLEESQKGLENIKEEKIKLEQKIENNKQESKKLELEL
                     ENLNKLEQEQKQKLESFDIQKIQELEN"
BASE COUNT          955 a          235 c          249 g          514 t
ORIGIN      
        1 aatatttata ttcaaaaaat aattttggat ttattggcaa tatcatatca aaatatctga
       61 cctttcaaag ataaaattat aaatattttt tttgataaag gaaaattttt gatcaaagct
      121 cctattggta gtggaaaaag ttttctgttt tttgatggac cagtttatgc tctttacaaa
      181 aatactggta gaaatgtttt gaatgtagac agcaaaaact gattcattaa attgttgttt
      241 gaaataaatg gacaaaaata tttgatagtc agaaatctta caaaatgacc ggtaaaagat
      301 tcatgcactt ctcaactgtt tttggtgcac aacctaatat gaggagtttc aaaagaatct
      361 gatcaagata tacacgaaat tttgaaaaaa accgattgag taaacttgga agaaattgtt
      421 tttaaaaatg aaagagattt agaatgaaat ttatctacta ttttgccacc tagagaggct
      481 tttttgagta ctatgttttt attacaagat agtgaaaata tttttgaact ccaagctgct
      541 gaaagactga ctgtaatgaa aaatattttc aatctaattt gaatagatga aatcaaggac
      601 caaatcgcaa acaaaaggag agatattcaa accacattaa aagtaaaatc tgacacaagt
      661 aattatgatt taaaaatcaa aaatatatta caaaacctta tccaaaattt caaaaaaata
      721 gacattccag aaactgatga aaatctgaag actgcccttt caaacaacca agaactaatc
      781 caagaacgag agttaatagc agataacata aatatagatt gatttagtgt tcaatctttt
      841 gataaaaact taaatacaga actaaacagc catatctgaa aacaaaaaag ctcttaccaa
      901 gaattttttg taaaacttca acacacacaa gaacaaacaa aaaacaaaca agaaaacatc
      961 aaaatcctaa acaatgattt aatagatttc caaaaacaaa tagaaaattt tgataaacaa
     1021 atagaaaata tagatccaga aattatagac aataagaaaa aagaaaaaat aaatttatta
     1081 aaagagagtg ataaaatatt ggattgagtt aatttacaac acgatatccc aaatgagatt
     1141 cacgaattca tttcaaaaaa accaaatgat atagaagatt ggaacaatct agtgattgaa
     1201 ttgactacta aatgacagga tttagccaat caaaaaaaga atacagaatt aatggttcaa
     1261 aataaaaaac taaaaattaa atctgaaaat gagaaaaaag aaattgaaat aaaaaatcta
     1321 aatgaaactc aaaaaactct aaaaaataat ttgaataata ttgttgatag aatcaacaat
     1381 ttgaacaaat cgatcaacga agaatctcaa tacaaatgtt cacaaataaa tgatttttgt
     1441 ccttttatta aagaaataaa caaacaaaca tttcaagaat tagaaaaaca aaaagaaagt
     1501 tttgaaaaag aaaaacagga attagaaagt aaaatagctg aaattcaaga aagaatccaa
     1561 aataacaata agataaaccc agatcaagaa ttagaacaaa attcaataga aaatctggaa
     1621 aatgaaatta aaaaaataga agaatcaata aacaatttga agacattttt tacaaaaatt
     1681 gagttcaaaa aaattcaaga aacctacaat caacgaaaaa atatccaaac agaaattagc
     1741 aagttagata aagaaatact acaattggaa gaatcacaaa aatgattgga aaatataaaa
     1801 gaagaaaaaa ttaaactaga acaaaaaata gaaaacaaca aacaagagag caaaaaacta
     1861 gaattagagc tagaaaactt gaataaattg gaacaagaac aaaagcaaaa actagaatct
     1921 tttgatatac aaaaaattca agaattggaa aat
//
LOCUS       DAOSID010000120         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_51730_length_1919_cov_17.228004, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000120 DAOSID010000000
VERSION     DAOSID010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51730_length_1919_cov_17.228004"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            335..907
                     /gene="grpE"
                     /locus_tag="PK674_03730"
     CDS             335..907
                     /gene="grpE"
                     /locus_tag="PK674_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOG15673.1"
                     /translation="MTDKDLQEKLKQAQKEHKQKADSKKEHALQIEIEKLQKNYQEQT
                     EIAAKAQRDYINLKLDFDRYQNQVSEREKEQDTNSLISSVKKFLPFIEDLRKSLDNIP
                     QDKKSDPLAQGVQLVYDNFLKVLESMHIFQIKALGLVPDSLVHEPVSLMPVEDKKMKG
                     KIIQEFERGFIYKKGDKQIVINPSKVIVGN"
     gene            complement(939..1703)
                     /locus_tag="PK674_03735"
     CDS             complement(939..1703)
                     /locus_tag="PK674_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG15674.1"
                     /translation="MSQKDFYNNNVKKFSIYKSTYNQTNEHTQQFIFNLIGTHKHKII
                     LDYGCGNGGISYLHAKAGAKLVLGVDISKKLIANAIKTKQNINSLHFLTIDDKNLYFD
                     KVIKDGIFDIITSYLVFCCIKDEKLLNSIIKSFYYKLKKGGKVYIQLPNIEDFNGKES
                     YSFYIIKENNLQEGKETFAYLRTNGKAFPKGLNNSSEHLKIINYFWSKKKLKEMFINQ
                     GFKNVEIYNIKVENSEYDGIVDEKNYSPLFVLTATK"
BASE COUNT          699 a          237 c          276 g          707 t
ORIGIN      
        1 gaaaatactt ggctacagaa ttgtttgaaa aggatttaag ttttgcggag tattatgcgt
       61 taagtttgta aaattacaag ttgaaagttt ataaagttga caactatccc ccttactcgt
      121 tacggagaac tccgtagata agggggatta agggggattt tagactaata gtggggttta
      181 gttttttttg gctcgcttac ttacagtttc ccgtaaataa ggggaattaa ataaacacct
      241 tcatttttct caaatccccc cttcccccct tattaagggg ggtcaaaaat ttaataaact
      301 gtaagaattt tttaactaat attataaata caaaatgaca gacaaagacc tacaagaaaa
      361 attaaagcaa gcacaaaaag aacacaaaca aaaagcggat tcaaaaaagg agcatgcttt
      421 acaaatagaa atagaaaaat tacaaaaaaa ctatcaagaa cagacagaaa tagctgctaa
      481 agcacaacgc gattatataa atctcaagct agattttgat agatatcaaa atcaggtttc
      541 agaaagagaa aaagaacagg atacaaattc tcttatttct agtgtgaaaa agtttttgcc
      601 ttttattgag gatttgagaa aatctttgga caatattcct caagataaaa aatcagatcc
      661 attagcacaa tgagttcaat tggtttatga taactttttg aaagttttgg aatctatgca
      721 tattttccaa attaaagcgc ttggactggt gccagattct ttagtacatg agccagtttc
      781 tttgatgcct gtagaagata aaaaaatgaa atgaaaaata attcaagaat ttgaaagagg
      841 ttttatttac aaaaagggtg ataagcaaat agtaataaat ccttcaaaag taattgttgg
      901 gaattaagtg ttaagtgttt ttttgaagta taaaaatatt attttgtggc tgttaacaca
      961 aataatggac tgtaattttt ttcatcgaca attcagtcat attcactatt ttctactttt
     1021 atattataaa tttcaacatt tttaaatcat tgattaataa acatttcttt taactttttt
     1081 ttggaccaga aatagtttat gatttttaaa tgttctgagc tattgtttaa tcacttgggg
     1141 aatgcttttc catttgtcct taaataagca aatgtttcct ttcactcttg taaattattt
     1201 tcttttataa tataaaaaga ataagactct ttcccattaa aatcttctat attgggtaat
     1261 tgaatataaa cctttcatca ttttttaagt ttataataaa aagattttat tatagaattt
     1321 agcaattttt catcttttat gcaacaaaaa actagatatg aagttataat atcaaaaatt
     1381 caatctttga taactttatc aaaatagaga tttttgtcat ctatggtaag aaaatgtaag
     1441 gaattaatat tttgttttgt ttttatagcg ttagcaataa gttttttaga aatatcgact
     1501 cataatacta attttgctca ggctttagca tgcagatagc ttattcctcc gtttcagcat
     1561 caataatcta gtattatttt gtgtttatgt gtaccaataa ggttaaatat aaattgttgg
     1621 gtatgttcat ttgtttggtt ataggtactt ttatatattg aaaatttttt aacattgttg
     1681 ttataaaaat ctttttgact catttgatgt gtttagaaat aaaaaaagaa tattcattta
     1741 gataaaacac tatgaaaaat ataaatattg cttatttctt ttcaaatcta ttttttagac
     1801 tattccaatt tttctgttga aatataaaaa tatatggata atttctgttt gattgtttat
     1861 tacactaaat tttcatgaaa ccagattttg atatagaaca tatcccacat aagccttga
//
LOCUS       DAOSID010000121         1913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_51968_length_1913_cov_14.282562, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000121 DAOSID010000000
VERSION     DAOSID010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51968_length_1913_cov_14.282562"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1763)
                     /gene="fusA"
                     /locus_tag="PK674_03740"
     CDS             complement(<1..1763)
                     /gene="fusA"
                     /locus_tag="PK674_03740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="elongation factor G"
                     /protein_id="HOG15675.1"
                     /translation="MAQVIPLDRVRNIGIMAHIDAGKTTFSERVLYYTGKKHKVGETH
                     DGAADMDWMDQEKERGITITSAATTCFWKDYHINVIDTPGHVDFTVEVERSLRVLDGA
                     VAVFDASQGVEPQSETVRRQADKYKVPRMAFINKMDKMGADFFMAVDSIKSKLTDKGI
                     VCQIPYGAADDFKGIIDLLDMKLYTFEGEMGVNVVEHEIPAELQAKAEEYREKMIDAA
                     TMFDDELADKFLEGGDIPKELIIKAIRKGTVSNELYPILCGTALRNTGVQLALDYVTR
                     YLPSPLDRGTIYGIDPDNEEKKLERHASPTEPVSALAFKIASDPFVGTLTFVRVYSGT
                     IKSGDSLLNPITGQKERVGRLLLMHANKREEISEIGAGHICAFLGLKDTRTGNTLCDI
                     KNPIILEKIEFPEPVINISIEPKTKDDQERLGLALAKLMYEDPTFRAHSDEESAQTII
                     SGMGELHLDIIVDRLKREHKVEVNTGKPQVAYRETIMADSVEGEGVFKRQTGGRGQYG
                     HVLLRLEKLQDKNYEFVSEIKGGVIPNEFIPAIDKGAKETMAQGILAGYPIINIRVVP
                     YFGSYHDVDSSEIAFKIATFRA"
BASE COUNT          590 a          384 c          241 g          698 t
ORIGIN      
        1 gctctaaatg tcgcaatttt aaacgctatt tctgaagaat ccacatcatg ataagatcca
       61 aaataaggaa ctactctaat atttatgata ggatatcagg ctaaaatacc ctgagccata
      121 gtttcttttg ctcccttatc aatagctggt ataaattcgt ttggaatcac tcatcattta
      181 atttctgata cgaattcgta gtttttatct tgaagtttct caagtctaag taatacatgt
      241 ccatattgtc ctctacctcc agtttgtctt ttgaatactc attctcattc tacactatca
      301 gccataattg tctctctata tgctacttgt ggttttcctg tatttacttc tactttatgt
      361 tctcttttta gtctatctac tataatatcc aaatgtaatt ctcacatacc agaaataatt
      421 gtttgagcac tttcctcatc agaatgagct ctaaatgtag gatcttcata cataagtttt
      481 gccaaagcta atcataatct ttcttgatca tctttggttt ttggttcaat agaaatgttg
      541 ataacaggtt ctgggaattc tattttttca agtattatag ggtttttgat atcacataaa
      601 gtatttcctg ttcttgtatc ttttaatcct aagaacgcac aaatatgtca tgctcatatt
      661 tctgaaattt cttctctttt gtttgcgtgc atcaaaagca atctccccac tctttccttt
      721 tgacctgtaa taggatttag caaagaatcc ccagatttaa tagttccgga ataaactcta
      781 acaaaagtca aagtaccaac aaatggatct gatgcaattt tgaacgctaa cgcagatact
      841 ggctctgtag gagaagcgtg tctttcaagt tttttctctt cattgtctgg atctattcca
      901 taaatagttc atctatccaa aggactaggc aagtatctag taacataatc taatgctaat
      961 tgaactcatg tatttcttaa tgctgtccca cataatattg gatataattc gttgcttact
     1021 gtaccttttc taatagcttt tatgattaat tcttttggga tatctcatca ttccaaaaac
     1081 ttatctgcca attcatcatc aaacatagta gcagcatcta tcattttttc tctatattct
     1141 tcagctttag cttgtaactc tgcagggatt tcatgttcta caacatttac tcccatctct
     1201 cattcaaatg tatacaattt catatctaac aaatctatta ttcatttgaa atcatccgca
     1261 gcaccataag gaatttgaca tacaattcat ttatctgtaa gttttgattt gatactgtca
     1321 acagccataa aaaaatctgc tcccatttta tccatcttat ttatgaaagc cattctagga
     1381 accttatatt tatccgcctg tcttcgaact gtttctgatt gaggctctac tccttgagaa
     1441 gcatcaaaaa ctgccacagc tcaatctaaa accctcagag atctttctac ttctacagta
     1501 aaatctacat gacctggagt atctataaca tttatgtgat agtctttcca aaaacaggta
     1561 gtcgccgctg atgtgatcgt aattcatctc tctttttctt gatccatcca atccatatca
     1621 gctgctccat catgagtttc tcaaacttta tgttttttac ctgtataata aaggactctt
     1681 tccgaaaaag tggttttacc agcatctata tgtgccataa taccaatgtt tctcacccta
     1741 tccaaaggaa tcacttgtgc catcttcaca aaaaataaaa taataaaaaa tgcaaaaaaa
     1801 attgcaatat cgaacaccct tataataaat tacttagctt tttcaagaca attttttata
     1861 atattaatta taattcaaaa agcaccacta atagataaga taataaatgg gaa
//
LOCUS       DAOSID010000122         1813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_55945_length_1813_cov_13.314562, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000122 DAOSID010000000
VERSION     DAOSID010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55945_length_1813_cov_13.314562"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..427
                     /locus_tag="PK674_03745"
     CDS             86..427
                     /locus_tag="PK674_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="YraN family protein"
                     /protein_id="HOG15676.1"
                     /translation="MSNKRKLGQIGEQLVIGYYEERGYEILDTNYVIPGGELDVIAQK
                     DGTIVFVEAKVVDYVDDLMGYIKPQKLKFLERTIEDYLYKKNLDFEVRLDVAFVKNNS
                     ILEVFENVTNS"
     gene            449..1660
                     /locus_tag="PK674_03750"
     CDS             449..1660
                     /locus_tag="PK674_03750"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="S41 family peptidase"
                     /protein_id="HOG15677.1"
                     /translation="MKKLKIVLIFLLGILIGGSIMRFGPNDFFDKIFVGTEEQSELDY
                     YKNKYNRFEALDEVLRLGYYDYEKINQDDMIKGAIKAYIDSIKDPYTVYMDAEQNSGF
                     LGSLEGEENFEGIGAAVSKKEYYIQIEEVLKESPAMKAGLRPLDRIIAINTGYVKNES
                     LDESIARIKGPAGTDVSLIIERYEKDDKREVFEVTITRENINLPSVESSVFEINGKKI
                     GYIEMFLIGEETENIFKKEVSKLKSEGVEGIILDLRGNGGGLMPVAVEIVSHFVPKGQ
                     LIVSAKYEGYTDEKYYSRGFGEFEGIKTVVLIDGMTASAGEIIAVALQEQAGAKLLGT
                     NTFGKGTIQTLDEFSDGDSLKYTIGKRFPASDKNIDNIGIKPDITVEFDMETYLENNV
                     DNQLEEAKSLF"
BASE COUNT          717 a          172 c          355 g          569 t
ORIGIN      
        1 tgaaaaaaaa cttttcattt aatattgaaa tattgtcgtg ttttctttaa tatgaaagat
       61 gattttttat ttttaataaa gaaaaatgag taataaaaga aagctgggac aaattggtga
      121 acaattggtt atttgatatt atgaagaaag aggttatgaa attttggata caaattatgt
      181 gatcccaggt ggagaactgg atgtaatagc acaaaaagac ggaacaatcg tttttgtaga
      241 agcaaaagta gtagattatg tagatgattt gatgtgatat ataaaaccac aaaaattaaa
      301 gtttttggaa aggactattg aggattactt gtataaaaaa aatctagatt ttgaagtaag
      361 acttgatgtt gcttttgtca aaaataacag tattttggaa gtgtttgaaa atgtaacaaa
      421 ctcatagttt ttataatata aacaaaaaat gaaaaaactt aagatagtat taatattttt
      481 actaggaatt ttgattggtg gtagtataat gagattttga ccaaatgatt tttttgataa
      541 aatctttgtt tgaactgaag aacaaagtga gttagattat tataaaaaca aatataatcg
      601 ttttgaagca ttagatgaag tgttgaggtt atgatattac gattacgaaa aaatcaacca
      661 agacgatatg atcaaatgag ctatcaaagc ttatattgat tctatcaaag atccttatac
      721 tgtatatatg gatgcagaac aaaatagtgg attcttggga agtttggaat gagaggaaaa
      781 ttttgaaggg atatgagctg ctgttagcaa aaaagaatac tatatacaaa ttgaagaagt
      841 tttgaaagaa tcccccgcta tgaaagcggg acttaggccg ttagatcgta ttatcgctat
      901 aaacacttga tatgtaaaaa atgaaagttt ggacgaatct attgctagga ttaaatgacc
      961 agctggaact gatgtaagtt tgataattga aagatatgaa aaagatgata aaagagaggt
     1021 ttttgaggtt acaattacta gagaaaatat taatttacca tcagtagaaa gtagtgtatt
     1081 tgagatcaat ggtaaaaaaa tatgatatat agaaatgttt ttgatatgag aagagacaga
     1141 aaatatattc aaaaaagaag taagtaaact aaaatctgag tgagtagagg gaattatatt
     1201 agatttgaga ggaaacggtt gatgactaat gccagtcgct gttgaaatag tatcgcactt
     1261 tgtaccaaag tgacaattaa ttgtttcagc aaaatacgaa tgatatacag atgaaaaata
     1321 ttattcaaga tgatttggag aatttgagtg aataaaaaca gttgttttaa ttgattgaat
     1381 gactgctagt gctggagaga ttatagctgt cgctttacaa gaacaagctg gagccaaact
     1441 tttgggtact aatacttttg gaaaaggtac aatccaaact ttggatgaat tcagtgattg
     1501 agatagtttg aaatacacaa tatgaaaacg attcccagct agtgataaaa acatagataa
     1561 tatttgaata aaaccggata ttacagtaga atttgatatg gaaacttatc ttgagaacaa
     1621 tgtggataac cagctagaag aagcaaaaag tttattttag tcgctgtagt cgttagacat
     1681 tagtcgttag tcgttagtct atagtcgtta gctaatgtct aatgactagc gactaaagac
     1741 tagtgactaa agactagtga ctaaaaaata tttactcttt aatatagtga taaaatgata
     1801 aaagtaatag tac
//
LOCUS       DAOSID010000123         1806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_56230_length_1806_cov_12.043975, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000123 DAOSID010000000
VERSION     DAOSID010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56230_length_1806_cov_12.043975"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..633
                     /locus_tag="PK674_03755"
     CDS             70..633
                     /locus_tag="PK674_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15678.1"
                     /translation="MTKSQKKIKGGGEQTFRLLVILLILSVLGLVFLKVSVFLLQKQI
                     NENRESLQQQEQQLTAYESATSYDKFLLVNDLEERITEMPWFEHIPKILQILQDIKNL
                     DPNSDDVITLSDFNVSLDEITLKGRVSTLKALYYNSPTSGGFKALLERFQELDFIKDI
                     TIRSYNRASDRNFEFILNAKVIDNDGK"
     gene            620..1423
                     /locus_tag="PK674_03760"
     CDS             620..1423
                     /locus_tag="PK674_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15679.1"
                     /translation="MTENKEILNKLIGSQENEALIPTSESDGAGQKSTITTSRIIGVR
                     YKFYTIILLLLIFIVGYNYILPSYERYESTKIELSDVELELSNFQTKKTKYESNIGLV
                     DTIKNVESQIVNCVNMLEGCQELPDIVKNNFSVVRSYLLLTEMSNNKMSLDEKKILAN
                     IDGFLLKRDSLSSDSAISNGVLNKIIIGEKEEFNDNLYFVPIELSIKFSDKDGLMSFI
                     DNVEKKIPVQEELRILYKIDKINYDIVNYDEPQESSIFMYGYFYDDKTK"
     gene            1424..>1806
                     /locus_tag="PK674_03765"
     CDS             1424..>1806
                     /locus_tag="PK674_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="type II secretion system F family protein"
                     /protein_id="HOG15680.1"
                     /translation="MINLFTPKNIEYKKHSVDNTIVHFLKSRDKSSFGTKDKIFFFKE
                     LAYMLKGGVGLVQAVDTIYNSTHNYAVKEISIGVKKYLELGKSLSYALNRLPDYFDEG
                     DYSVVRAGEKSGNLSIVLESLATEY"
BASE COUNT          716 a          215 c          276 g          599 t
ORIGIN      
        1 tattcaaata tttttgcaaa tattttattt atatttatac taaatatagt ttaattttta
       61 ataaaacaaa tgacaaaatc tcaaaagaaa attaaaggcg gtggagaaca aacattcaga
      121 ctattagtaa ttttactaat tctttctgta ctttgattag tttttctcaa agtatcagtt
      181 ttcttgcttc aaaaacagat aaatgaaaac agagaatctt tacaacaaca agagcaacaa
      241 cttacagcat acgaaagtgc tactagttat gataaatttt tattggtaaa tgatttagaa
      301 gaaagaatta cagaaatgcc ttggtttgag catatcccaa aaatattaca aattttacaa
      361 gacataaaaa atttagatcc aaattctgat gatgttatta ctttgtccga ttttaatgta
      421 agtttagatg aaattacttt aaaaggtagg gtttctacat tgaaggcctt atattacaac
      481 tctcctacat catgaggctt caaggcacta ttggaaagat ttcaagagct agattttatc
      541 aaagatatca caattagaag ctataacagg gcttctgata gaaactttga atttatttta
      601 aatgctaaag taatagacaa tgactgaaaa taaagaaatt ctaaacaaat tgataggaag
      661 tcaagaaaat gaagctttga ttcccacatc agagtctgat tgagcaggcc aaaaaagcac
      721 tatcacaact tccagaataa ttggagtgag atataagttt tatacaatta ttttattgtt
      781 attaattttt atagtttgat acaattatat tttacctagt tatgaaagat atgaaagtac
      841 aaaaattgag ctgtcagatg tagagctaga actctctaat ttccaaacta aaaaaacaaa
      901 gtacgaatct aatatatgat tggttgatac catcaaaaat gtagaatctc agatagtaaa
      961 ctgcgtcaat atgctagaat gatgccaaga gttacctgat atagtcaaaa acaatttttc
     1021 agtagttagg tcatacttac tactaacaga gatgtccaat aacaaaatga gtttggatga
     1081 aaaaaagatt ttagcaaata ttgattgatt tttattaaaa agagattcat taagctctga
     1141 ttctgcgata agcaattgag ttttaaataa aataattatt tgagaaaaag aagaatttaa
     1201 tgataattta tattttgttc cgatagaatt aagtattaag tttagcgata aagatggttt
     1261 aatgtctttt atagataatg ttgagaaaaa aatcccagtt caagaagaac ttcgtatttt
     1321 atataagatc gacaaaatta attatgatat cgtaaattat gatgaaccac aagaatcaag
     1381 catatttatg tattgatact tttatgacga taaaacaaaa taaatgataa atctttttac
     1441 cccaaaaaat attgaatata aaaaacattc tgttgataat acaatagttc attttctaaa
     1501 atctagagat aaatcgagct ttggtaccaa ggataagatt ttctttttta aagaactggc
     1561 atatatgttg aaatgatgag ttggtttagt acaggcagtt gatacaatct acaatagtac
     1621 acataactat gccgtaaaag agatttctat ctgagtaaaa aaatatttag aattatgaaa
     1681 aagtttgtct tatgcactaa acagattgcc agattatttt gatgagtgag actatagtgt
     1741 agtcagagct tgagaaaaat cagggaattt atccatagtt ttggagtctt tggcgacaga
     1801 atatga
//
LOCUS       DAOSID010000124         1746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_58955_length_1746_cov_15.128326, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000124 DAOSID010000000
VERSION     DAOSID010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58955_length_1746_cov_15.128326"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PK674_03770"
     CDS             <1..773
                     /locus_tag="PK674_03770"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=25
                     /product="pitrilysin family protein"
                     /protein_id="HOG15681.1"
                     /translation="SYGRSTLGPVENIKKFTREDLIRHKNGLYTKDNLILVIAGKIEN
                     QKKLENIIADTFQNLPEKSSIEKPEFKNILPSKQKDFYSKKTEQNHLIISAKGFDGHD
                     TKRYVANVLSTILGGNMSSRLFQNIREKQGLCYYINARHMSDSDSGVFIIRAGLEKGR
                     FDFGLEKIYEELESIAKGDISKEDFEKAIGYNIGQVQMGIESSDNMADFMGSQYLLYG
                     EIKTLDEILEIYRNMKLEDVLEITNKFKKENLYQYWIE"
     gene            complement(777..1586)
                     /locus_tag="PK674_03775"
     CDS             complement(777..1586)
                     /locus_tag="PK674_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOG15682.1"
                     /translation="MKITSRNVNGIRAVINKGFDERFKKDNPDILCIQEPKAFEHQIP
                     AELKFITRDYNYIRHSGTKSGYAGTAIFYKKDLKVISTKNHFGELEQFHEDGRVVEMK
                     FHGKDNKDSVFTLLNIYFPNGGTKADGTEMLTYKLEFYENFIQYINNLRANGENIITT
                     GDFNICHHPIDIARPEENKDSIGFLPIERKKLDKLQANGYVDVFRHFNPDLKDKYTRR
                     SYRAGARPRNVGRRLDYFRVSENILPLITHIDHQDLVMGSDHCPISLELDI"
BASE COUNT          622 a          241 c          283 g          600 t
ORIGIN      
        1 atagttatgg acgaagtact ttatgaccag tagaaaatat aaaaaaattt actagggaag
       61 atttgataag gcacaaaaat ggtctttata ccaaagataa tttaattttg gtaatagctg
      121 ggaaaattga aaatcagaaa aaacttgaaa acattattgc tgatactttt caaaacttac
      181 ctgaaaaaag tagcatagaa aagcctgaat tcaaaaatat tttaccaagc aaacaaaaag
      241 atttttattc caaaaaaaca gaacaaaatc accttataat ttctgctaaa tgatttgatg
      301 gtcatgatac aaaaagatat gttgctaatg ttttgtctac gatacttgga tgaaacatgt
      361 catctagatt attccaaaat ataagagaaa aacaatgact ttgttattat atcaatgcta
      421 ggcatatgag tgattcagat agctgagttt ttataattag agcatgattg gaaaaaggaa
      481 gatttgattt tggtttggaa aaaatctatg aagagctaga aagtatagca aaatgagata
      541 taagtaaaga agattttgaa aaagcgattg gatacaatat ttgacaagtc caaatgtgaa
      601 tagagtcgag cgacaatatg gcagatttta tgtgaagtca gtaccttttg tattgagaaa
      661 taaaaacttt agatgagata ttagaaatat acagaaatat gaaactggaa gatgttttgg
      721 aaattactaa taagttcaaa aaagaaaatt tatatcagta ctggattgag taaaatttat
      781 atatccaatt ccaaactaat tggacaatga tcagatcaca tcacaagatc ctgatgatca
      841 atatgagtaa tcaatggtaa aatattttct gatactcgga aataatccaa tctccgtcaa
      901 acatttcttg gtctagctca tgcacgataa gatcgccgag tatatttatc tttcaaatct
      961 gggttaaaat gacgaaaaac atccacatat cagtttgctt gtagtttgtc cagctttttt
     1021 ctttctatag gaaggaatca tatagaatcc ttattttcct ctggtcttgc tatatctata
     1081 ggatggtgac agatattgaa atctcatgtc gtaattatat tttctcaatt tgctctaaga
     1141 ttgttgatgt actgaataaa attttcataa aactctagct tgtaggttag catttccgta
     1201 ccatctgcct tagttcatca attggggaaa taaatattca aaagagtgaa aacggaatct
     1261 ttattatctt tcccgtgaaa tttcatctct actactctac catcttcatg aaattgttcc
     1321 aattctcaaa aatgattttt tgtggaaatt acttttaaat cttttttgta aaaaatagct
     1381 gtaccagcat atcatgattt tgtcccagaa tgtcggatat aattgtaatc tcttgtaata
     1441 aatttcaatt ctgctggaat ttgatgttca aatgcttttg gttcttgaat acataaaata
     1501 tctggattgt cttttttgaa tcgctcatca aatcatttat ttattactgc tctgattcag
     1561 tttacatttc gtgaagttat tttcatagtg agttggtaag tttataaagt atttatatta
     1621 aagggaatga ttatagaagt acggtctgtt ctcctttgct ttgctgataa gttgtttact
     1681 acttatttct taaatcaccc caaacccctc ttcgattacg gagttctccg taacaagcga
     1741 agaggg
//
LOCUS       DAOSID010000125         1729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_59800_length_1729_cov_18.927718, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000125 DAOSID010000000
VERSION     DAOSID010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59800_length_1729_cov_18.927718"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="PK674_03780"
     CDS             complement(<1..1244)
                     /locus_tag="PK674_03780"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="TIGR00341 family protein"
                     /protein_id="HOG15683.1"
                     /translation="MKIGKHIRIWWLEGDTNSDLDKKEIEVKDNVPECDGVEVGADSN
                     KKCNVAKSTRFFDKIALLKLDDSKKSEIAQQIINDSYSTKLYWIQLFLACVIAALGLF
                     MNSIPVTIGAMLISPLLDPIKSLSFAITNGHKKMYFRSIKILLLSILVAIISSVFVSI
                     IVPFSHITPEITSRISPTILDLFVALFSGAFAFLSLGFKKLEETVAGVAIAVALLPPL
                     SVVGIGMYFLDFSIIQGSFLLFLTNLVAIIIVGVIILYLFGFFPTNRAGQKRSLIVFL
                     MVFFFVLLIIFPLKKSMLQISDNIKINNQINETIKSYFKSIDTKIYLDKVSFQNLGDD
                     LIRINSVLNVPSDVLITNENKTDLTKILSLNLQKSIELDLSIVGISSVYISKEPTKEQ
                     LMMSKVKNIFDSTFKGIVLLDY"
     gene            1373..>1729
                     /locus_tag="PK674_03785"
     CDS             1373..>1729
                     /locus_tag="PK674_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HOG15684.1"
                     /translation="MILALAPMDGITDLACRVITKKIFEKYNKNPEYQFTLRTEFMTS
                     DGYVANPDKVIKHIIHTDFEDPIVLQIFGGNKKKLLETALDIEKKYGNKIFGIELNTG
                     CPANNVMKSGGGSALLK"
BASE COUNT          696 a          250 c          214 g          569 t
ORIGIN      
        1 ttataatcca ataaaacaat tcatttaaat gtactatcaa aaatattttt cactttggac
       61 atcatcaatt gttcttttgt tggttctttt gaaatatata cactagaaat tccaactata
      121 ctcagatcca actcaataga tttttgtaaa tttaatgata atatttttgt caaatcggtc
      181 ttattttcat tagtaattaa aacatcactt gggacattca aaacagaatt gatacgaatt
      241 aaatcatctc ataaattttg gaaagaaacc ttatctaaat atattttcgt atctatagac
      301 ttaaaataag attttatagt ttcatttatt tgattgttta ttttaatatt atcagaaatt
      361 tgcaacatag attttttaag agggaaaatg ataagcagaa caaagaagaa caccatcagg
      421 aatacaatca aacttctctt ttgtcaagcc ctattggtcg ggaagaatca aaatagatat
      481 aatataatca ctcaaacaat aattatcgcc actaagttag ttaaaaacaa taagaaacta
      541 ccttgaatta tagaaaaatc taaaaaatac attcatattc aaacaacaga aagtggtggt
      601 aaaagtgcaa cggcaattgc aacaccagca acggtttctt ctaatttttt aaatcataaa
      661 gataaaaaag caaatgctca tgaaaataaa gccacaaaaa gatcaagaat agtgggagaa
      721 atcctagatg taatctctgg tgtaatatga gaaaatggaa caataattga aacaaataca
      781 ctagatataa tagcaaccag aatactcagt aacaatattt ttatacttct aaaatacatt
      841 tttttatgac catttgtaat agcaaatgac aatgatttga taggatctaa aagcggtgaa
      901 ataagcatag ctcagattgt aacagggata gagttcataa acaatcataa ggcagcaata
      961 acacaagcca aaaataactg tatccaatat agcttagtag agtatgaatc atttattatt
     1021 tgttgtgcta tttcactttt tttggaatca tctaatttca ataatgctat tttatcaaaa
     1081 aatctagtag acttagctac attacatttt ttattggaat cagctcctac ttctacacca
     1141 tcacactctg gtacattgtc cttgacttca atttcttttt tatctagatc agagttagta
     1201 tctcattcca accaccaaat tcttatatgt tttcaaattt tcataaaaac attcacaaaa
     1261 tataaatcaa ttcaattata tacaaaaaca taaaaaaaag caaatttcaa attgttttaa
     1321 tttttcaaaa aactaaacta aagttgtttt tagacttata aattagaatt taatgatttt
     1381 agcactagct cctatggatg ggattactga cctagcctgt agggttataa caaaaaaaat
     1441 atttgaaaaa tataataaaa accctgaata tcaatttact ctacggacag aattcatgac
     1501 atcagatggc tatgtcgcga atccagataa agtaataaaa cacataattc atacggattt
     1561 tgaagaccca atagtattac aaattttttg atgaaataaa aaaaaacttt tggaaactgc
     1621 tttggacatt gaaaaaaaat actgaaataa aattttttga attgaattga acactggttg
     1681 tccagcaaat aatgttatga aatcctgatg atgatctgct ttattaaaa
//
LOCUS       DAOSID010000126         1708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_60922_length_1708_cov_21.392619, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000126 DAOSID010000000
VERSION     DAOSID010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60922_length_1708_cov_21.392619"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..587)
                     /locus_tag="PK674_03790"
     CDS             complement(102..587)
                     /locus_tag="PK674_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=25
                     /product="hypothetical protein"
                     /protein_id="HOG15685.1"
                     /translation="MKKSKFQINVSDLLISGGKKDEITFENESLEELENLSKDGISGT
                     VIIQSFDNGSLLVNLKNLTCTIVEPCDKCTESYSREVFVPKYQAKFQIQIDPEDDEDD
                     ETYLIENENIDIQEMILNAVRLEEPFSKLCTNCTKDSLDMDEDDEDEYESFGLGNVQF
                     R"
     gene            705..>1708
                     /gene="tilS"
                     /locus_tag="PK674_03795"
     CDS             705..>1708
                     /gene="tilS"
                     /locus_tag="PK674_03795"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOG15686.1"
                     /translation="MKSIKNIKDLSITDRAEFYGVFEQLSNYICPETKILIAVSGGAD
                     SMFLSFLIYSFFAKNNLDFNNLFFIHCNHKTRKETEQEQKFIEDFFDGLNLNIVIFYS
                     GNEKKTENTLRNWRYSEFQKVIDKNKIDYIFTGHNLTDRIESSFMNIFRGSGLNGFLS
                     MKFIDKSPLLQNVQTVRPLLSFTKIQIEEFCSKFNVPFFVDQTNLDSSTSLRNKIRLE
                     LFPQFEKLSNTKESFYQSMLNIYSELENLENLDLGNLLEIKKSYYWNSDYAFLRDIPI
                     PKISQSTILQLLKNLNLSSNITEKTLNDLYDFFQNSKQGYKYINGTYFFLSHSKIYII
                     "
BASE COUNT          617 a          235 c          207 g          649 t
ORIGIN      
        1 cgaaaattat aaaatttgta actttacaaa ccccgccatg tagtactata agtggcgggg
       61 caggcttgta actttataaa cttgtaactt gtgactcatt cctatctaaa ttgaacattt
      121 cataatcaaa aactttcata ttcatcttca tcatcttcat ccatatccaa actatctttt
      181 gtacaattag tacataattt actaaaaggt tcttccaatc tgactgcatt caaaatcatt
      241 tcttgaatat ctatgttttc attttcaatc aagtaagttt catcatcttc atcatcttct
      301 ggatctattt gaatttggaa ttttgcctga tatttcggta caaaaacttc tctactgtaa
      361 ctttcggtac acttatcaca aggttctacg attgtgcaag ttaaattttt tagattaacc
      421 aataaggatc agttatcaaa agattgaata atcactgtac cgcttatgcc gtcttttgat
      481 aaattttcta attcttctaa actttcattt tcaaaagtaa tttcatcttt tttgccacca
      541 gatattagta aatctgatac attaatctga aattttgatt ttttcattgg atttagtttt
      601 gaaattaata taaatagatg cgataaaatt atttcaatac tatattagca aaaaatatag
      661 tatattttat caagtactat atatcatttt acgaattatt ttcaatgaaa agtattaaaa
      721 atattaaaga tttaagcatc actgatcgag ctgaatttta tggtgttttt gaacaactat
      781 caaattatat ttgtccagaa acaaaaatat taattgctgt tagtggatga gcagatagta
      841 tgtttttgag ttttttgatt tatagttttt ttgccaagaa taatttagat ttcaataatt
      901 tattttttat tcactgtaac cacaaaacaa gaaaagagac tgaacaagaa caaaaattta
      961 ttgaagattt ttttgattga ttaaatttga atattgttat tttttatagc gggaatgaaa
     1021 aaaaaacaga aaatactttg agaaattgga gatatagcga gtttcaaaag gtgattgata
     1081 aaaataaaat tgattatatt tttacctgac acaatttaac tgaccgtata gagtctagtt
     1141 ttatgaatat ttttcgtgga tctggcttaa atggattttt atcaatgaaa tttatagata
     1201 aatctccatt acttcaaaat gtgcaaactg tccgtccatt gctatctttt acaaaaattc
     1261 aaatagaaga gttttgctct aaatttaatg ttccattttt tgttgatcaa actaacttag
     1321 attctagcac aagcttgaga aacaagatca gattagagct gttccctcaa ttcgagaaat
     1381 tatcaaatac aaaagaatct ttttatcaaa gtatgctaaa tatttattca gaacttgaaa
     1441 atctagaaaa tttggattta tgaaacttat tggaaattaa aaaatcttat tactggaata
     1501 gtgattatgc ttttttgcga gatattccaa ttcccaaaat atctcaaagt acaattttac
     1561 aacttctaaa aaatctgaac ttatcatcaa atattactga aaaaacatta aatgatttat
     1621 atgatttttt tcaaaactca aaacaaggat acaaatatat taatgggact tatttttttc
     1681 tttctcactc aaaaatctat attatcaa
//
LOCUS       DAOSID010000127         1696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_61569_length_1696_cov_16.823278, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000127 DAOSID010000000
VERSION     DAOSID010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61569_length_1696_cov_16.823278"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /gene="thyA"
                     /locus_tag="PK674_03800"
     CDS             <1..549
                     /gene="thyA"
                     /locus_tag="PK674_03800"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="thymidylate synthase"
                     /protein_id="HOG15687.1"
                     /translation="LGPIYGYQRRNFGGEYISRDQESQTKGVDQLKKMIETLKTDPND
                     RRMIVMARNPQFLHRMALPPCHYGFQVLVSDGKLHLLRNQRSVDTMLGLPFNIASYAL
                     LLHLLAKESGLKEGKLIGFLADTHIYTNHIEGAKEQINRTPLKLPTIKTEKFTSIFDR
                     EYTDTVVENYEHQGKIKFDIAV"
     gene            complement(695..979)
                     /locus_tag="PK674_03805"
     CDS             complement(695..979)
                     /locus_tag="PK674_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOG15688.1"
                     /translation="MKEYYVYMLASDSGTLYIGMTNNLKRRIYEHKEGLLDGFTKKYG
                     CKKLVYYEIGNDVSHIIMREKQLKKWSRKKKEFLINLLNPKWRDLYNEIF"
     gene            complement(1037..>1696)
                     /locus_tag="PK674_03810"
     CDS             complement(1037..>1696)
                     /locus_tag="PK674_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="ATP-binding protein"
                     /protein_id="HOG15689.1"
                     /translation="PGEVSLAHKGVLFFDEITEFPRETLEVLRQPIEDKNISISRVSG
                     SVNYPANFMFVASMNPCKCGYYKDPERACTCSINEVKNYQNKISGPLMDRIDMILEIP
                     RENIDKLLDNVKSESSQSIREKIIKARQIQKKRFAGTNIVSNSDMSSNDIEKYIPLSD
                     EIKEFLTTASERLVLSPRVVHRIIKLARTIADMEGVESLEIKHLAEAMQYRSKSMFVE
                     R"
BASE COUNT          592 a          321 c          215 g          568 t
ORIGIN      
        1 ctttgaccaa tatattgata tcaacgaaga aatttttgat gagagtacat tagccgagac
       61 caagaatctc aaaccaaatg agtagatcaa ttaaaaaaaa tgatagaaac attaaaaaca
      121 gacccaaacg atagaagaat gattgttatg gctcgaaatc ctcaattttt acatagaatg
      181 gcattacctc cttgtcatta tggatttcaa gttttagtat cagatggtaa attacaccta
      241 ttacgaaatc aaaggtctgt agatacaatg ctttgattgc cgttcaatat agctagctac
      301 gcattactac tacatctact agccaaagaa tcttgattaa aagaaggaaa actaattgga
      361 tttttagctg atacccacat atacacaaac cacattgaat gagctaaaga acaaatcaac
      421 agaactccat taaagttacc aacaataaaa actgaaaagt tcacttcaat atttgaccga
      481 gaatacacag atacagtagt agaaaactat gaacatcaat gaaaaattaa atttgatata
      541 gccgtataag cgagattata ggtttataaa gttacaaatt ctataccacc tgtcccgtag
      601 tactacgggg cggagctaca ttttatagag attaaaacca taaactgtca tttcgactaa
      661 gtgaacaaag tgaacgcacg gagaaatcct tttattaaaa aatttcatta tataaatccc
      721 tccatttagg atttaataag ttaatcaaaa actccttttt tttcctactc cattttttca
      781 attgcttttc tctcattata atatgactaa catcgttacc tatttcataa tataccaatt
      841 tcttacatca gtatttttta gtaaatcaat ctaataatca ttctttatgc tcgtatattc
      901 ttctttttaa attatttgtc ataccaatat ataaagtgcc agaatcactc gccagcatat
      961 aaacataata ttctttcatt gttatggtgg gaatataaag gatttctcga cttcgctcga
     1021 aatgaccgga atggccctac ctctctacga acatactctt actcctatat tgcatagctt
     1081 ccgctaaatg cttaatttct aagctttcta ctcactccat atcggcaata gtcctagcaa
     1141 gtttgataat cctatgaaca actctaggag ataaaactaa tctttctgaa gctgtagtca
     1201 aaaattcctt aatctcatca gacaatggga tatatttttc aatatcatta ctactcatat
     1261 cagaattgga aactatattt gtgccagcaa atcttttttt ctgtatttgc cgagccttta
     1321 taattttttc tctaatagac tgactacttt ctgattttac attatccagt agtttatcta
     1381 tattttctct aggaatttct aaaatcatat ctatcctatc catcaaagga ccagaaattt
     1441 tgttttgata atttttgact tcattgatac tacaagtaca agccctttct ggatctttgt
     1501 aatacccaca tttacaaggg ttcatacttg ctacaaacat aaaatttgca ggataattga
     1561 cactaccaga tactctagaa atactaatat ttttatcctc aattggctgt ctaagcactt
     1621 ccaatgtctc tctaggaaac tctgtaattt catcaaaaaa caaaactcat ttatgtgcta
     1681 agctcacttc tcatgg
//
LOCUS       DAOSID010000128         1647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_64210_length_1647_cov_16.808417, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000128 DAOSID010000000
VERSION     DAOSID010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64210_length_1647_cov_16.808417"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PK674_03815"
     CDS             complement(<1..460)
                     /locus_tag="PK674_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="regulatory protein RecX"
                     /protein_id="HOG15690.1"
                     /translation="MATQEEKTLYSDCCQYALKYISVYPKTEKELRIKLQQKGYNSDE
                     IRYTMDYLKSKKFADDRVFAESYIRSELVNKGKPVIAVTNKLYQKGVDKKLINEVIKD
                     YQEDISVGVFQKIRKEIDSYKKRGVDGFDIIQKLMRKGYRLEDIKTVIKLK"
     gene            complement(463..1083)
                     /locus_tag="PK674_03820"
     CDS             complement(463..1083)
                     /locus_tag="PK674_03820"
                     /inference="COORDINATES: protein motif:HMM:NF039585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HOG15691.1"
                     /translation="MGDFDVNYIVDKYGDTIKEEINVKDIQSFGDDIKITKVFKPLGS
                     ALSAKFAKDTGNIIKYGKMGNITELGEGRIKVFDDSGNEWILEKEDYEISYQGLEGDD
                     VAIDGGVIAKLDLQLTPELEMEGMAREVSRFLNQMRKDVDYNVDDKVKMFYHTNDEYM
                     VSVITKFSEFLSKEALLESIESSKQEGDIISIFNIDEKTVTFSLKK"
     gene            complement(1318..>1647)
                     /locus_tag="PK674_03825"
     CDS             complement(1318..>1647)
                     /locus_tag="PK674_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="pyruvate kinase"
                     /protein_id="HOG15692.1"
                     /translation="SEIIRAADAVMVARGDLGAEVPIETIPSHQLNIVGKVKRKGKKV
                     IVATQMLETMMNNCIPTRAEVTDVFYAVLQGADYLMLSGETSAGKYPIETVEVMNRII
                     AEGKKFI"
BASE COUNT          535 a          307 c          169 g          636 t
ORIGIN      
        1 cttttaactt gataactgtc tttatatctt ccaacctata accttttctc ataagttttt
       61 gaataatatc aaatcaatca actcatcttt ttttgtaaga atctatttct tttctaattt
      121 tctgaaaaac accaacagat atatcttctt gatagtcttt tatcacttca tttatcaatt
      181 ttttgtctac tcatttttga tataatttgt tagtcacggc aattactggc tttcatttat
      241 ttaccaattc gctcctaata taactttctg caaaaactct atcatcagca aatttttttg
      301 atttgagata atccatcgta tatcttattt catcactatt atatcatttt tgttgcaatt
      361 taattctcaa ttctttttca gtttttggat aaacagaaat gtattttaaa gcatactgac
      421 aacaatcaga atataatgtt ttctcttctt gtgttgccat aattattttt ttaaactaaa
      481 agtaacagtt ttttcatcaa tattgaatat agaaataata tctcattctt gttttgagct
      541 ttcaatagac tccaaaagcg cttctttaga caaaaattct gaaaactttg tgataacaga
      601 aaccatgtat tcatcatttg tatggtaaaa catctttact ttgtcatcta cattgtaatc
      661 cacatctttt ctcatttgat ttaaaaatct agaaacttct ctagccattc attccatttc
      721 aagctctgga gtgagctgta aatctaattt tgctataact ccaccatcga tagccacatc
      781 gtctcattcc aaaccttgat aagatatttc atagtcttct ttttccaaaa tccattcatt
      841 gccactatca tcaaaaactt ttatccttca ttctcaaagt tctgtgatat ttcacatttt
      901 tccatattta ataatattac cagtatcttt tgcaaattta gcagacaaag ctgatcataa
      961 aggtttaaat acttttgtaa tttttatatc atcaccaaaa gattgaatat cttttacatt
     1021 tatttcttct ttgattgtat ctcaatactt atctacaata taattcacat caaaatctcc
     1081 cataattaat aattttaaat aataaaaaaa cagttattat aacataatac tgtttatata
     1141 tttgtaaaaa attttcaaca gataataacg aacttcgttc gtgttaaaaa gataaaagga
     1201 taaaatgtta aagtaaatta tcgtgagcaa agttcacacc gcttaagcga tgcgaacaat
     1261 ttatcatttt atcgcaagca aagcgagcat ttatcatttc atcatgagcg aagcgactta
     1321 tataaacttc ttcccttctg cgataattct attcattacc tctactgtct ctatcggata
     1381 tttaccagct gaagtttctc cagacaacat caaataatcc gctcattgca gaaccgcata
     1441 aaaaacatct gtaacttctg ctctagtcgg gatacaatta ttcatcatgg tttctagcat
     1501 ttgagtggca acaataacct tttttcattt tctttttact ttcccaacaa tatttaattg
     1561 atgagaaggg attgtttcaa tagggacttc tgctcataaa tctcacctag cgaccatcac
     1621 agcatcagca gctctaataa tttcaga
//
LOCUS       DAOSID010000129         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_65119_length_1630_cov_13.692063, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000129 DAOSID010000000
VERSION     DAOSID010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65119_length_1630_cov_13.692063"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..1550
                     /locus_tag="PK674_03830"
     CDS             324..1550
                     /locus_tag="PK674_03830"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOG15693.1"
                     /translation="MFQVKYSSGLRGEVTIGASKNASLPIIAANYLLDNKVKLTNKPG
                     IVDVQVLEEIFEEAKSKSTDFFDLTMEKATKIRTSILLIPYGLCRHKTVKFIGSGGCN
                     IGKRPLDTFDDGLVQAGIKITNDDFKTYEIFTKPKKNIVMQEFSVTAIEALLTYLAFA
                     DNFDYDINIYQVAIEPHVRNLIKFLNNAGANITLNVDHSIVVKPSKIQIKNHEFTIIP
                     DYIAAGTYFAIGAGADDSEITIKNVDVDDLSSMYSIANRIGIDFKIIDKHTIRVNSFN
                     KKNYKAIKLETRIYPGFPTDLQSIFGTLLTQANGVSKIFETLFEGRFGYLSELENLGA
                     KVEILNPHQAIVIGPIKLKGGYVTSTDLRGGGAMVLAGIMAKGTTNIMNENIILRGYD
                     NIVENLQNIGVDIVHI"
BASE COUNT          591 a          261 c          278 g          500 t
ORIGIN      
        1 aagtttgtaa aaaatttctt cccttaactc cgataaatca ggcttcggga agtttcttta
       61 tcagtacaaa cacagaatcc tcccccattg gggaagttgt tctagtactg cggggattac
      121 aacctccccc tagggaggaa ctttatcatg aaattcttcc ctcgggggaa gttgttctag
      181 tgtagttggg ggtagttgga gcaaaacccc cacctatccc acaccgtaag actacggtgt
      241 gggaaagcat cctcccccaa gggagggatt ctaattgaga actttacaaa ctttatagtt
      301 ttttatcttt taatcaattt tttatgtttc aagtaaaata ctctagtgga cttagatgag
      361 aagtgaccat ttgagctagc aaaaatgcat cgttaccaat aattgcagca aattacttac
      421 tggataataa ggtaaaacta acaaacaaac catgaattgt agatgttcaa gtattagaag
      481 aaatctttga agaagcaaaa tctaaatcaa cagatttttt tgatttgacc atggaaaaag
      541 ctaccaaaat tagaacatca attttactta tcccgtacgg attatgtaga cacaaaacag
      601 taaaatttat tggaagtggc ggatgtaata tttgaaaaag acctttggac acatttgatg
      661 attgactggt acaagctggt atcaaaatta ccaatgatga tttcaaaact tatgagattt
      721 ttacaaaacc aaagaaaaac attgtgatgc aagaattttc tgtaactgct atagaagcct
      781 tgctgactta tttagctttt gcagacaatt tcgattatga tatcaatatt taccaagtag
      841 cgattgagcc acatgttaga aatcttatca aatttttgaa caatgctggg gcaaatatca
      901 cattaaatgt agatcatagt attgtagtta aaccatccaa aatccaaatc aaaaaccatg
      961 aattcactat tatcccagat tatatcgcag catgaacata tttcgctatt tgagcatgag
     1021 cagatgatag tgaaattact atcaaaaatg tggatgtgga tgatttatct tctatgtata
     1081 gcatagcaaa cagaatttga atagatttta aaataataga taaacatact attagagtaa
     1141 attcatttaa taaaaaaaac tataaagcta ttaaattgga aactaggatt tatccaggat
     1201 tccctactga cctacaatca atatttggta cattgcttac gcaggccaat tgagtgtcca
     1261 agatatttga aactcttttt gaatgaagat ttggttattt aagcgaacta gaaaatttat
     1321 gagcaaaagt agaaatatta aaccctcatc aagcaatagt tatttgacca attaaactaa
     1381 aatgatgata tgtgacttcc acagacctta gatgatgatg agctatggta ctagcatgaa
     1441 ttatggccaa atgaactaca aatatcatga atgaaaacat tattttaaga tgatatgata
     1501 atattgtaga aaatctccaa aatatttgag tagatattgt acatatttag gaaataagct
     1561 cgccccagct ccaccacgta gtaactacgt agtttagcgg ggttaacttt gttcgcgata
     1621 aaatgataaa
//
LOCUS       DAOSID010000130         1621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_65653_length_1621_cov_17.136015, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000130 DAOSID010000000
VERSION     DAOSID010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65653_length_1621_cov_17.136015"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1611)
                     /locus_tag="PK674_03835"
     CDS             complement(<1..1611)
                     /locus_tag="PK674_03835"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=25
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOG15694.1"
                     /translation="MKPDFDIEHIPHKPGVYQFKDKKGSILYIGKAKNLKNRISQYFN
                     PNSVWKQEMLNLAETVDFFQLKTESEALYLEDNLIKKHQPPFNNMLKGSNSYAYIKIT
                     NEDFPQIIVTRNKIKDGSKYIGPKHNTRELKNFLQYLRYILKWRGCKKTEFKKGKLCS
                     DYHFGLCKGRCSAQQFPNPEKAKKEYSDIVFLIEQFFRGNTKPVEKEIKNQIKIATKQ
                     QYFERAAKLRDIYLQIENLVEKQTVVLPKNIVGYICQIREISGWLVYVVLNFYEGKLI
                     DVIVNKKNLEDFDENSVLSSLESELGNLTKQEFHGIDFYYTEKIKLNKTQISDINTLI
                     DGFFDSYLVGESFKDENMLNGLLSQIKSKYSLKNFPYQIECIDISHFSGSNTSGGLSC
                     FVGGVPNYKLYRKYKIDAKIDTKSGDDYSALMELITRRFKDTQNLPDLFIIDGGKGQL
                     GILKELLGQERFQSIYAKVDFVSLGKGVARKTGGKLKGEKEKIFRFDDSFKIQQMDLD
                     YDQADKILVKVRDEAHRFANSYRKEQMKK"
BASE COUNT          512 a          257 c          212 g          640 t
ORIGIN      
        1 ttttttcatc tgctcttttc tataagagtt ggcaaatcta tgtgcttcat cacgaacttt
       61 tacaagaatt ttatctgcct gatcataatc taaatccatt tgttgaattt tgaatgaatc
      121 atcaaaacgg aatatttttt ctttttctca tttgagtttt catcaagttt ttctagccac
      181 tcattttcct aatgatacaa aatctacctt ggcataaata gactgaaatc gttcctgtcc
      241 cagcaattct ttcagtatcc caagctgtca ttttcatcaa tcaattataa ataaatctgg
      301 taaattttga gtatccttga accttctagt aataagctcc atcaaagctg aataatcatc
      361 accagacttt gtatcaattt ttgcatcaat tttatattta cggtacaatt tgtaatttgg
      421 cactcatcaa acaaaacacg aaagtcatca acttgtattt gaaccagaaa aatgagaaat
      481 gtcaatacat tctatttgat aagggaaatt tttcagagaa tatttggatt tgatctgtga
      541 aagtaatcaa ttcaacatat tttcatcttt gaaagactct cagaccaaat aagaatcaaa
      601 aaacccatca ataagtgtat ttatatcaga aatctgagtt ttatttaatt ttattttttc
      661 tgtataataa aaatcaattc aatgaaactc ttgtttagtc agatttcata gctcagattc
      721 cagtgatgat aacactgaat tttcatcaaa atcttccagg ttttttttgt tgacaattac
      781 atcgataagt tttcattcat aaaaatttaa tacaacataa acaagccaac ctgaaatttc
      841 tctgatctga caaatatatc aaacaatgtt tttgggaagg actactgttt gcttttctac
      901 taagttttct atttgtaaat aaatatctct aagttttgct gctcgttcaa aatattgttg
      961 ctttgtggcg atttttattt ggttttttat ttctttttct actggttttg tatttcatct
     1021 aaaaaactgt tcaatcaaaa aaactatatc tgaatactct ttttttgctt tttctggatt
     1081 tgggaattgt tgagccgaac atcgtcattt acataatcaa aaatgataat ctgaacaaag
     1141 ctttcatttt ttgaactctg tttttttgca tcacctccat ttcaaaatat atctcagata
     1201 ctgcaaaaaa tttttgagct ctctggtatt atgttttggt cagatatact tagatcaatc
     1261 tttgatttta tttctagtca caataatttg aggaaaatct tcatttgtga tttttatata
     1321 agcataacta ttggatcatt tcagcatatt attgaaaggt ggctgatgtt ttttgatcaa
     1381 attatcctcc aaataaagtg cttctgattc cgttttcaat tgaaaaaaat ccactgtttc
     1441 agcgagatta agcatttctt gtttccaaac tgagttggga ttgaagtact gagaaatcct
     1501 atttttgaga ttttttgctt ttcagatata gagaatactc ccctttttat ctttgaattg
     1561 ataaactcaa ggcttatgtg ggatatgttc tatatcaaaa tctggtttca tgaaaattta
     1621 g
//
LOCUS       DAOSID010000131         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_67714_length_1586_cov_19.080340, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000131 DAOSID010000000
VERSION     DAOSID010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67714_length_1586_cov_19.080340"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..>1586
                     /gene="lysS"
                     /locus_tag="PK674_03840"
     CDS             48..>1586
                     /gene="lysS"
                     /locus_tag="PK674_03840"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="lysine--tRNA ligase"
                     /protein_id="HOG15695.1"
                     /translation="MGVVPYAQSFEKTHLISQVISEFGDKKHRDINDIIPNPDIQIKT
                     AGRVMLHRSHGKLAFLRLLDSTGQIQLMFHRDNCKIDVSISDDQPKLLETLETEGEEM
                     SAYKFMEKMIDVGDFIGVRGEVFTTHKGELTIFVSEFKFLAKAIRPLPEKFHGLTDQE
                     ELYRKRYLDMIMNPESYERFLFKSKFYHTLREFYYKEGFTEIQTSILCNAASGAAAKP
                     FITHHNDFDTEFYLRIAFETALKKATVGRFEKVFEIGQDFRNEGSDPSHLQEFTQVEH
                     YAAYRNYEDNMKFTEKMFDYIFDNMGLNRVIPVKDKDGNIKEVDFTTPWERIDYTKGI
                     LEASGIDITQYGQGDADKLRADIKAKGIEFERMDTMGTTTLIDYLYKKVLRPKILGPA
                     FIYNYPVIMQPLARISDKDKGIVEQFQLLVNGREICKAYSELVDPVLQQANFDAQKEA
                     AAQGDEEATAEDKDFVTAMEYGMPPQSGFGMGLERILAILTQQDNLRDVVMFPIMKPE
                     TDEEL"
BASE COUNT          617 a          219 c          277 g          473 t
ORIGIN      
        1 tatccgagag agatgttaga atccaaaaaa ccaaaacaat gtctaaaatg tgagtagtgc
       61 catatgcaca aagttttgaa aaaactcacc ttatttctca agtaatttca gaattttgag
      121 ataaaaaaca tagagacata aatgacatta ttcccaatcc tgatatccag ataaagacag
      181 caggtagagt tatgcttcat cgtagtcatt gaaaattagc atttttgaga ttacttgatt
      241 ctacatgaca aatacaactt atgttccata gagacaattg taaaatagat gtttcaatat
      301 cagatgatca accaaaatta ttagaaacat tggagacaga gggtgaagaa atgtcagcat
      361 acaaatttat ggaaaaaatg atagatgttt gagattttat ttgagttaga ggagaggtct
      421 ttactactca taaatgagaa ctgaccattt tcgtttctga atttaagttt ttggcaaaag
      481 caattagacc attgccagaa aaattccatg gactaacaga tcaagaagag ctatatagaa
      541 aaagatattt ggatatgatt atgaatccag aatcttatga aagattttta ttcaaatcca
      601 aattctatca tactttgaga gagttttact ataaagaatg atttactgaa atccaaacat
      661 caattctttg taatgcagct tctggagccg cagcaaaacc atttatcaca caccacaacg
      721 attttgatac tgaattttac cttagaatag cgtttgaaac cgctcttaaa aaagcaacag
      781 tttgaagatt tgaaaaagtt tttgaaatat gacaggactt tagaaatgaa tgatcagatc
      841 cttctcattt acaagaattt actcaagtag aacactatgc agcataccga aattatgaag
      901 acaatatgaa gtttactgaa aaaatgtttg attatatatt tgataatatg tgattaaata
      961 gagtaatccc tgtgaaagat aaagattgaa acataaaaga agtggatttt acaacacctt
     1021 gggaaagaat agactataca aaaggtattt tagaagctag ctgaatagat atcactcaat
     1081 attgacaagg agatgctgac aaacttagag cagatatcaa agcaaaagga atagagtttg
     1141 aaagaatgga tacaatggga acaacaacat tgatagacta tttatataaa aaagttctta
     1201 gaccaaaaat actttgacct gcctttatct acaattatcc agtaattatg caacctttag
     1261 ctaggatttc agataaagat aaatgaattg ttgagcagtt tcagcttttg gtaaatggac
     1321 gagaaatttg taaagcttat tcagaattag tagatccagt attacagcaa gcaaattttg
     1381 atgctcaaaa agaagctgct gctcagtgag atgaggaagc aactgcagaa gataaggatt
     1441 ttgtaactgc tatggaatat tgaatgcctc ctcaatcttg atttggtatg tgattggaaa
     1501 ggattttagc aatattaact caacaagata atttaagaga tgttgttatg ttcccaatta
     1561 tgaagccaga aactgatgaa gaactt
//
LOCUS       DAOSID010000132         1553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_69764_length_1553_cov_17.353138, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000132 DAOSID010000000
VERSION     DAOSID010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69764_length_1553_cov_17.353138"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1553
                     /locus_tag="PK674_03845"
     CDS             <1..>1553
                     /locus_tag="PK674_03845"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOG15696.1"
                     /translation="LDEAMDPTNHIILFIDELHTIIGAGGQDNNDAAQMLKPLLARGK
                     IKLIGATTFDEYQKHIEKDAALKRRFQEVIVNEPDGQTAIQIMEGLKQTYEDFHGVSI
                     SNSAIKASVKLTKRYMLNKYLPDKALDIIDEACARKSTMQYKLENDEEYKKIEKKIDK
                     IKDEIEVAIENQDYFKAAELKEKEEELKNDILKIRNNKNIPSHLRPTIEKEDIGNVLA
                     DKTGIPANVVNQSEIEKLKMLADSLKGQVFGQDDAVQAVVNTLTRNRLSVIEKHKPIG
                     SFLFLGPSGVGKTFLAKMIAKSYFGDESAIVRLDMSEYMEKFSVSKLIGSPAGYVGYD
                     EGGQLTEAIRRKPYSVLLLDEIEKASLDVLNILLQILDEGKLKDSKGRWVDFKSTIIV
                     MTSNLGSEQFSKKGGTIGFSTGETKKGEIDEKTFEKAQERVLEELKDFMSPELINRID
                     HKIVFKPLSKEVLTDIFKKNLKEFLDSWKANSKAVLPEYTEKEIKEIIDKIYDPQYGA
                     RPVERYIHDT"
BASE COUNT          662 a          177 c          293 g          421 t
ORIGIN      
        1 ttggatgaag ctatggatcc tacaaaccat ataattttgt ttattgatga attacataca
       61 attatatgag ctggatgaca agacaacaat gatgctgctc aaatgttgaa accactatta
      121 gctagatgaa aaatcaagtt aatttgagca acaacttttg atgagtacca aaagcatata
      181 gaaaaagatg cggcactaaa aagaagattc caagaagtga ttgtaaatga gcctgatggt
      241 caaactgcta tacaaattat ggaagggctg aaacaaactt acgaagattt tcatggagta
      301 agtatcagta attctgctat caaagctagt gtaaaattga caaaaagata tatgttaaac
      361 aaatatttac cggataaggc cttggatatt attgatgaag cttgtgctag aaaatctaca
      421 atgcaatata agttggaaaa tgatgaagaa tacaaaaaaa ttgagaaaaa aatagataaa
      481 atcaaagatg aaatagaagt agctattgaa aatcaggatt atttcaaagc agctgaatta
      541 aaagaaaaag aagaggagtt gaaaaatgat attttgaaaa ttagaaacaa caaaaacata
      601 cctagtcatc taagacctac tattgaaaaa gaagatattg gaaatgtttt agctgacaaa
      661 actggaatcc cagcaaatgt agtaaaccaa tcagaaatag aaaaactaaa aatgttggct
      721 gattccttga aatgacaggt gtttggacaa gatgatgcag tacaagctgt tgtaaacact
      781 cttactagaa atagactgtc agtaatagaa aagcataaac caattggttc atttttattt
      841 ttgtgaccaa gtggtgttgg aaaaacattt ttagctaaaa tgatagccaa aagttatttc
      901 ggagatgaaa gtgcaatagt taggctagat atgtcagaat atatggaaaa attctctgta
      961 agtaaattga taggtagtcc agctggatat gtttgatatg atgaatgagg acaattaaca
     1021 gaagcaataa gaagaaaacc atattctgta ttacttttgg atgaaataga aaaagcatca
     1081 ttggatgttt tgaatatttt attacaaata ttggatgaat gaaaattgaa agatagcaaa
     1141 ggtagatggg ttgatttcaa aagtacaata atagtaatga cttcaaatct tgggagtgaa
     1201 caattcagta aaaaatgatg aacaattgga ttcagcacag gagaaactaa aaaatgagaa
     1261 atagatgaaa aaacttttga aaaagcacaa gaaagagtat tggaggagtt gaaagatttt
     1321 atgtcgccag aattgataaa tagaattgat cataaaattg tattcaagcc gttatcaaaa
     1381 gaagttttaa ctgacatctt caaaaagaat ttgaaagaat tcttggattc ttggaaagca
     1441 aactcaaaag ctgttttacc agaatataca gaaaaagaaa taaaagaaat aattgacaaa
     1501 atttatgatc cacaatatgg agcaaggcca gtagaaagat atatccacga tac
//
LOCUS       DAOSID010000133         1510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Absconditabacterales bacterium isolate
            AMR_MDS_1710 NODE_72741_length_1510_cov_18.658419, whole genome
            shotgun sequence.
ACCESSION   DAOSID010000133 DAOSID010000000
VERSION     DAOSID010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863239
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Absconditabacterales bacterium (wastewater metagenome)
  ORGANISM  Candidatus Absconditabacterales bacterium
            Bacteria; Candidatus Absconditibacteriota; Candidatus
            Absconditabacterales.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:06:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 770
            CDSs (total)                      :: 739
            Genes (coding)                    :: 737
            CDSs (with protein)               :: 737
            Genes (RNA)                       :: 31
            tRNAs                             :: 30
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Candidatus Absconditabacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72741_length_1510_cov_18.658419"
                     /isolate="AMR_MDS_1710"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2927939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..>1510
                     /gene="dnaK"
                     /locus_tag="PK674_03850"
     CDS             30..>1510
                     /gene="dnaK"
                     /locus_tag="PK674_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=25
                     /product="molecular chaperone DnaK"
                     /protein_id="HOG15697.1"
                     /translation="MGKVIGIDLGTTNSCVAYMLGDKSEVIANSEGARTTSSIVYIKG
                     DELLVGDLAKRKAILEPKNVIFETKRFIGRKFKEVKDEVKDMPYDMREGSDGGIIITV
                     DKKDYKPEQLAGFILKKIKEDAEKFLGEPVTQAVITVPAYFNDSQRNATKAAGEIAGL
                     KVERIINEPTAASLAYGEGKNKNEKIVVFDLGGGTFDVTILEIGSEGTFQVLSTSGDT
                     QLGGKDFDQKIINYLIDAFKTKEGIDLRGDAMAMQRLKDEAEKAKMQLSQVERVDIMI
                     PFICTDDKGQPKNLAESLTRAQFENLSKDLLDRCKQPVIQALKDSKLDKSDINEIILV
                     GGSTRMPSIKKIVKDIFDKEAKSTINPDESVAQGAAIQGGIIQGDVTDILLLDVTPLS
                     LAVEVEGGLAHVLIERNTTIPAKKSNIYTTATDNQSAVTVHVTQGERQFAKDNKSLGQ
                     FNLEGIPPMRRGQPQIEVTFDIDANGILKVSAEEKSTGKKQDVT"
BASE COUNT          589 a          222 c          284 g          415 t
ORIGIN      
        1 aaaaatttta attattaatt ttataaaaaa tgggaaaagt aatttgaatc gatctaggaa
       61 caaccaactc ttgtgttgcc tatatgcttg gagataagtc agaagtaata gcaaatagcg
      121 aatgagctag aactacatcc tctattgttt acatcaaggg agatgaattg ttggttggag
      181 acctagcaaa aagaaaggct atattggagc caaaaaatgt aatttttgag accaaaagat
      241 ttattggtag aaaatttaag gaagtaaaag atgaggtaaa agacatgccc tatgatatga
      301 gagaatgatc tgacggtgga ataattatca ccgttgataa aaaagattac aaaccagaac
      361 aactagctgg ttttatattg aaaaaaatca aagaagatgc agaaaaattt ttgtgagaac
      421 ctgtaactca agctgttatc actgtccctg catattttaa tgattctcag agaaatgcta
      481 ccaaagctgc tggagaaatc gctggtctaa aagttgaaag aatcatcaac gagccaactg
      541 cagcatcatt agcttatgga gaatgaaaaa ataaaaacga aaaaattgtg gtttttgatc
      601 tttgagggtg aacttttgat gtgacgattt tggaaattgg atcagaaggt actttccaag
      661 ttttatctac atccggagat actcaacttg gaggaaagga ttttgatcaa aaaataatca
      721 attatttgat agatgctttc aaaaccaaag aaggaattga cttaagatga gatgccatgg
      781 ccatgcaaag gttaaaagat gaagcagaaa aagctaaaat gcaactttcc caagtagaaa
      841 gagtagatat tatgattcca tttatctgta ctgatgataa atgacaacct aaaaacttag
      901 cagaatctct tactagagcc caattcgaaa acttatcaaa agatctattg gatagatgta
      961 agcaacctgt aattcaagca ctaaaagata gtaaactaga taagtctgat atcaacgaaa
     1021 ttattttagt tggaggatct actagaatgc cttcaattaa aaaaatcgta aaagatatct
     1081 ttgataaaga agcaaaatct actatcaatc cagacgaatc tgtagctcaa ggagctgcta
     1141 ttcaatgagg aattatccaa ggagatgtga ctgacattct tcttttggat gtgactcctc
     1201 tatcacttgc tgtagaagtt gaatgatgat tggcgcatgt tttgatagaa agaaatacta
     1261 ccatacctgc aaaaaaatca aacatttaca ctaccgcaac agataaccaa tcagcggtta
     1321 ctgtccatgt tacacaatga gaaagacaat ttgcaaaaga taacaaatct ctctgacaat
     1381 ttaatttgga agggattcca ccaatgagaa gaggtcaacc tcaaatagaa gttacatttg
     1441 atattgatgc aaacggaatc ttaaaagtaa gtgctgaaga gaaatctact ggtaaaaaac
     1501 aagatgttac
//
